LOCUS       DAOOLO010000001        40048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_749_length_40048_cov_6.922436, whole genome shotgun sequence.
ACCESSION   DAOOLO010000001 DAOOLO010000000
VERSION     DAOOLO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 40048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40048
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_40048_cov_6.922436"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..2127
                     /gene="rnr"
                     /locus_tag="PLH96_00005"
     CDS             100..2127
                     /gene="rnr"
                     /locus_tag="PLH96_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPB60194.1"
                     /translation="MSSRSPKTVKKHSQKPKKPLTGVIRVTAGAFGFIDQGRRPTPTK
                     PSSAKSAKGFSKTQRKSGPKKNETVFIPPGALNTALDGDEVEYVMTRGDNGEVLKVLK
                     RARTEFVSQVTGRENGFLIVKPDNPKFYTDLVIEETAETAALSIGAKIVARLKLWPKS
                     TLRPGGDFIKNLGQSGEHETEMQAIIQDRGLIEGFSVELETEAQKIAQEHNLDAEIKK
                     RKDFREVTTFTIDPLAAKDFDDALSIRQTADGLWEVGIHIADVSHYLTPGSLLDLEAQ
                     KRATSIYLVDRTIPMLPKILSDDLCSLVPHENRLTFSAVFEMDDKANIKSEWFGRTVI
                     KSDRRFTYEEAHKIIETNQGEYAAELRKLDSLAKELDRQKISAGALAFEEDEVSFEID
                     ALGKPLNVFKRERLGTHKLVEDFMLLANKKVAEYVSRLAKNQNGVFVYRVHDAPDPDK
                     LDGVRRFINLFGHKLTHKGSRPGPQEINNFLKKIAGQPEESVITREIIRSMSRAVYST
                     KNIGHFGLAFAHYTHFTSPIRRYPDVMVHRLLDTYLKGQSPAKDLLLRYDKLCDHSSS
                     MEKLATDAERESVKYKQAEYLENHLGEVFDGVISSLTKWGMYVEELSTGAEGLIRYRE
                     MAGDRFIFEEKKFQVRGEKTNQIYRLGGLVKIKVAKTDRVTKTIDFTLDTD"
     gene            complement(2260..4518)
                     /locus_tag="PLH96_00010"
     CDS             complement(2260..4518)
                     /locus_tag="PLH96_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB60195.1"
                     /translation="MTLKRLEINGFKSFGRKAVLLFDAPITAVVGPNGSGKSNVAEAF
                     RWVLGEQSLKSLRGKKGEDLIFNGSSSLGRLSRASVSVTFDNSKRAFAGIDFDEVNIT
                     REVFRDGTNEYSINNSKVRLKDILALLAGISLGSSGHHIISQGEADRILNANLVERRA
                     MIEDALGLKIYQWKLEESEKKLTKTRENIKEAEILRREIAPHLKFLKKQMEKVEKAKE
                     LRAELATFYSEYLKREGFYLAYTRDKLKKELAIPKEILLATEEKLSALAAKEVSAPDE
                     AVEEKNKLREIEATLREGQAKKDDLSRRLGRLEGMIELKQRESRSATPTVDKLTVSRD
                     DLKRLEAELEGEWQKAKTENNPAVLKRILQNIRQILGEFWGGFKLHQLAPTDNLEMIK
                     ELLVEKQEKEKALAELERLEQSRQEEYQLAKQRLEQRENQEREEERESYELRARRGEL
                     AAVVRTLEGKLETLAVEEENFKRELGEAGVLIGREALNYEDYPLDEQEALSEKRATQE
                     ERHRRLERLKIRLEDIGGEGVDVEKEYDEVLARDGYLEKELLDLATSADSLEQVMSEL
                     RQRISDEFKAGVKKINSEFQKFFEILFGGGSASLSLVIPEKRRSRLDLASSDDLAPLG
                     ETMEDSDEPTEEGIEIIVSLPRKKIKGLAMLSGGERALTSIALLFAMSQVNPPPFMIL
                     DETDAALDEANSRKYGDMIENLSQFSQLILITHNRETMSRAGVLYGVTMGSDSVSRLI
                     SLKLTEAEQYAK"
     gene            complement(4590..5282)
                     /gene="rnc"
                     /locus_tag="PLH96_00015"
     CDS             complement(4590..5282)
                     /gene="rnc"
                     /locus_tag="PLH96_00015"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB60196.1"
                     /translation="MNKSLSEFEKNIGLDFADKKLLKQAFVHRSYLNEHRGFVFDHNE
                     RLEFLGDAVLELATTDFLFKKYPNKPEGELTSYRAALVNANTCSALALELGVNELLFL
                     SKGEAKDTGRARQIILANTFEAIIGAIYLDRGYEAAFNFIQKYLLDRAEEIIKKTIVE
                     DYKSYFQKKAQEVYGITPSYQLLKEIGPDHNKVFTIALFIGEEQVAVGEGLSKQEAEQ
                     EAAKSGLAKKGW"
     gene            complement(5263..6186)
                     /gene="nusB"
                     /locus_tag="PLH96_00020"
     CDS             complement(5263..6186)
                     /gene="nusB"
                     /locus_tag="PLH96_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB60197.1"
                     /translation="MANRHLSRSLAMQALFEWDFGDGKIKDLDETIDRIVAEFAPGLE
                     EKNFIKEIASGVIKHRAQIDKIIEKAAPDWPLEQIATVDRNVLRLGLYELIFGDRDDV
                     PAKVAINEAIELAKTFGGENSGRFVNGVLGTVYKEMGEPGKDDTPAKKKKIKDIPYEE
                     MPIEKLGGAVVFCEDKGEIKLAFVHDVFGFWTLTKGHIEEGESVKEGIKREAKEELGL
                     DVVIGQELGSNEYIASDPEKGKTRRQVKYYLAKCEPGEIKLAVKGGLDGASWFTFAKV
                     PELKIYDDIVPIITKAIKILAEKVKNEQEFV"
     gene            complement(6240..6449)
                     /gene="rpmF"
                     /locus_tag="PLH96_00025"
     CDS             complement(6240..6449)
                     /gene="rpmF"
                     /locus_tag="PLH96_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB60198.1"
                     /translation="MRHTRAHTGNRRSHHALKAPRLSKCANCGEAKASHRVCPNCGSY
                     RGREVVNVLAKTEKKEAKKKKAAAK"
     gene            complement(6619..7026)
                     /locus_tag="PLH96_00030"
     CDS             complement(6619..7026)
                     /locus_tag="PLH96_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60199.1"
                     /translation="MKLQKGRLEGTTKSQEINLALAKRVVRLFQQGEIARPTFDILVE
                     RVGLKSAIVLLAQEAKGKTEGVVELLLSDMELWVMVLMSPQLSYVGFSVLSRKNLSPE
                     SARRLLGQIQYVDLKYRDAVRRNCVRAIRTQVA"
     gene            complement(7098..7706)
                     /locus_tag="PLH96_00035"
     CDS             complement(7098..7706)
                     /locus_tag="PLH96_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPB60200.1"
                     /translation="MEIPKGVKYLVGIDEAGRGALAGPVAVGMFVWPLEVGDLLSATP
                     DYPVGRDSKKLTPRMREKWFAFLTNLTKQTGALCQVSLVAETYIDRAGIVPAVARGLE
                     ACLEKLPGEPIEYFLKLDGSLKAPEKFVYQETIIKGDETEPIISFASILAKVSRDKKM
                     TKMATKFAGYGLEKHKGYGTKEHFAKVAELGLSACHRRSYFA"
     gene            7841..11077
                     /locus_tag="PLH96_00040"
     CDS             7841..11077
                     /locus_tag="PLH96_00040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB60201.1"
                     /translation="MFVHLHNHTHYSLLDGLIKIPDLVKKAKEYNMPALAITDHGNMY
                     GAVEFYQECQKQDIKPIIGVEVYVAARSRFDKEPRIDSRRYHLTLLAKNETGYRNLIK
                     LVTKANLEGFYYKPRVDWELLQDHTKGVICLSGCMGGQLSRLLLNKDHAGAEKLARDY
                     AEAFGPDNYYIEIMHHPKIEGQIELAKNLIALARKLNLPLVATHDTHYLNKDDSRAHH
                     TLLAIQTGNDVDDVKGVTNMEEDFSFVSAEEMASFFPDTPDALENTLKISEACNLELV
                     LGKFVFPNFELDDDKTADEVLRQLAFSGLAKRGLDKDEAAIARLNHELEVIKIKGYAS
                     YFLVVEDLIRFARENNIYYNIRGSVAGSLTTYSLDITKVNPLAYHIPFERFLNPERPS
                     APDIDMDFADNKRDKIIDYAKQKYGEEKVAQIGTFGTMMARGSVRDVARALGFPYQAG
                     DQIAKLIPMGSQGFPMTIDRALEMVPELKEMYNERADTKEIIDMAKKIEGCARHISVH
                     AAGVVIAPAPLDQYVPLQYDPKGVSVITQYDMYSVGEDGVGLTKFDFLGLRNLAILSS
                     AVEIIETVYNRRVDMDGIPMDDKKTFEMLARGETEGLFQLNGSGMTKWLKELRPTTIH
                     DINAMVALYRPGPMQFIPDYIARKHNPRLIKYLDPSLESILEQSYGVLVYQDDLLIMA
                     NKLAGYSWGEVDKFRKAVGKKIPELMAKQREKFIKGCIEHSGWKKEKAEEVWAWLEPF
                     AAYGFNKAHSASYGRVAYQTAYLKANYPSEYMTAVLSAESGDVEKVAVIIGECKRMNI
                     PVLPPDINESFGDFTVIKNAQDPNLSEKAKKLANKNPDPVDKIRFGLFTIKNLGAEIA
                     QFIVDERKKNGPYRDLGDFLTRVKHKNLNKKFLEALAKAGALDSLGESRGKFLANLEE
                     ILAFNREEARNNANQTSLFGLMSDDAPKPTLKLRDADPIEPRLKLAWEKELLGLYVSG
                     HPLDAHREKFNKLENTIGHQKNKSEGFPIVTGGIIGEVRAIFTKNNKQMAFFKLSDFN
                     DMVECVAFSDVYEKYKALLVEDKCIALKGKLNYRNGEPSVLVEAIKEL"
     gene            11481..13223
                     /gene="thrS"
                     /locus_tag="PLH96_00045"
     CDS             11481..13223
                     /gene="thrS"
                     /locus_tag="PLH96_00045"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPB60202.1"
                     /translation="MENIEQIRHSLAHLLAMVVKEKYPAAKLGLGPAIENGFYYDFDL
                     GEKSLTDTDLDELEKKMRSLISQNLDFATETITADKACEIFAAEPYKLELIDELEKNS
                     ESISIYRSGDFVDLCAGPHVANSSDIPRDSFRLDRLAGAYWRGNENNKMLTRVYGLAF
                     TSKPELNDFLDKRAEAEKRDHRKLGKELDLFCFSDLVGPGLPLFTPKGTIIKEELQKE
                     VESICRHYGFEKVSAPSLAKQELFEISGHAKKFGEELFHVTSEKKHDLVLKPVQCPHH
                     TQIYASKSRSYKDLPIRYMESDKQYRAEKTGEVSGLSRVYAITVEDGHSFCRVDQVKE
                     EIKNMVSIIRDFYSRLGLWDEHWVSLSVRDYEHPEKYIGEPKDWDTCEQILQEVSNEM
                     GLEAKRCEGEAALYGPKLDFMFKDATGREIQIPTVQIDFATPKRFNLEFTNEQGEKVP
                     PVMVHRAILGSYERFLVLLIEHYAGAFPLWLSPVQAKILPISEKQMVYAKEILAELKA
                     RNIRVELDENNETLGKKIRNAKMEKIPYLLVVGDKEMESKTVTVEARGANKGESVSLA
                     VFCAKIEEGLKSRK"
     gene            13255..13782
                     /locus_tag="PLH96_00050"
     CDS             13255..13782
                     /locus_tag="PLH96_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60203.1"
                     /translation="MLPYKKILVLALILTAAWSAGSVLAEEATSTEDTASSTEQTVET
                     TDLDELINEFQVLRAKLLKALIAEFNANNLNEKQVQLLQVLLASDFSIYPEGVITGKV
                     GDFTKAAVRRYMESQGWNVPARLLQAGAGKSGNIPTGLKTAPGIQKKLQIASGTPTTT
                     PVQRNFLYRLKNIFR"
     gene            complement(13845..15914)
                     /locus_tag="PLH96_00055"
     CDS             complement(13845..15914)
                     /locus_tag="PLH96_00055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HPB60204.1"
                     /translation="MVKKELKNRKYDAEEIVVLEGLEPVRKRPGMYIGSTGVDGLHHL
                     LVEIFDNSRDEAMNGSCDEIEVALLPGDVVRIVDNGAGIPVDIHKKTKVSALETVLTI
                     LHAGGKFGDGGYKFSGGLHGVGASVVNALSEWLRAEIHRNDGKYVQEYERGKKKYNVK
                     RVSASKLHGTIITFKPDEQIFSETKYSWDRLVNHLRGQAYLVKSLRIRLIDARGYEGK
                     IDLDKIFWLRDLGLPVPSKTFFFEGGLLSLIKFYNQKEKPLHREIFYADKEQNGVFVE
                     IALQYVDDIDVKEISYTNNTVTPEGGMHVTGFRTALTRLLNDYAKKNNVLKDSDGGLT
                     GDDVREGLVAVISVKLLNPQFEGQTKTKLGSVEARSAVDAVFSEMFSAFLEEHPDDAK
                     AIINKGILALKARKAAKAAKDSIIRKGALEGMTLPGKLADCQSRDPEESEIFIVEGDS
                     AGGSGKQGRDRRIQAVLPLKGKILNVEKARLDRMMAFAEIRSLIIAMGVGIGDMMDLT
                     KLRYHKIIIATDADVDGAHIATLLLTFFFRYYRELIEQGKIFIAQPPLYKIKKGREFF
                     YAYNDEEKNKIIKELGGLTGEVEEGEAEEVEPEMAVEGEEDKEESSAKTRTAKISIQR
                     YKGLGEMNPEELWETTLNPETRVLKQVAIHDFDDADKTFDTLMGNEVGPRKLFIQTHA
                     KTANLDI"
     gene            complement(16045..17973)
                     /locus_tag="PLH96_00060"
     CDS             complement(16045..17973)
                     /locus_tag="PLH96_00060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB60205.1"
                     /translation="MLFSYNWLKKHVPDLPEPEKLEDGIIFHAFEVEGLAKAEGDWTL
                     DIKVLPDRAHDCLCHLGIAREAAAIFDLPLTKPIWEEVKPGDLEPLLIRIEDEKACRR
                     YVGRRVNNVAVFESADWLKKSLAVIGQRSINSVVDIANYVMFDLGQPLHAFDADKVEG
                     GIKARFAKPGEKIITLDGDEVALDESIFIIADDVGPLAIAGIKGGQKAGVTKETKNLI
                     LEAANFDPVLIRKIASKIKLKTDASKRFENELARETASEAMKMFSAMLKELVGGEYGE
                     VVDVYPAERQLSPIKIKPEFISQRLGVTIPEGEIKKIFSRLGLDVKEEDDGWLVAPPF
                     WRLDLTEEVNLVEEIGRIFGYEGIKPEILPPLSAKAYRAEHFEQQFLVANLVRRALFK
                     SGFTEVYGYAFTDHGEVKVENPLAADKAFLRANLIDWLGTKLKENLIHVIFDDEPVKI
                     FEIGTVFPQDGWEEMRLAIGLGYRKKIKGTEPPKELEMAVASVAEAIGWSGNLPRVTE
                     SASADGLHQLVEFNFEKMVDMAGPVSALPEKEMLNQDASYRPVSPYPRIIRDVAVWVP
                     ADMGAEQVGEIIIEAAGELLLDKPILFDEFKKEGRKSLAFRLVLQSYEKTLSDGEANK
                     VAEGVIGALEKADFEVRK"
     gene            complement(17977..18780)
                     /locus_tag="PLH96_00065"
     CDS             complement(17977..18780)
                     /locus_tag="PLH96_00065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB60206.1"
                     /translation="MVSPSKKGLIQYSKLNTQGHIHPLTQIINEVVGIFRELGFTVAD
                     GPEIEDEYHNFDALNVPAGHPARDMQDTFWLPDGKHLLRTQTSAVQVRYLAEMLKQGG
                     EPPLRILAPGKVFRQEATDARHESQFHQIEGLLVDENVSVAHLKGYLEKFFSGLYSQK
                     TPVRLRPSFFPFVEPGFEFDLVCIKCRDEKDEPIRGNACSLCSGTGWIEMGGAGLVHP
                     HVFRSVGLADKKWSGFAFGIGVERLAMLKWGVEDIRIFFNGDIRFLQQF"
     gene            18925..18997
                     /locus_tag="PLH96_00070"
     tRNA            18925..18997
                     /locus_tag="PLH96_00070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18958..18960,aa:Thr,seq:tgt)
     gene            complement(19001..19426)
                     /locus_tag="PLH96_00075"
     CDS             complement(19001..19426)
                     /locus_tag="PLH96_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60207.1"
                     /translation="MDNIILITICLLSLTGLVILLAWRVSCNRRNHLDFNQVADYSIA
                     HLVVDYLAFRLVYFCKQTISKGYLFLLYFLRNSISVIRYLIIRLEKRFYRFTESIKEK
                     EKVIHQGKVSSFLKEIREHKENVMAGNREGFLAEGEEVK"
     gene            complement(19437..19919)
                     /gene="tsf"
                     /locus_tag="PLH96_00080"
     CDS             complement(19437..19919)
                     /gene="tsf"
                     /locus_tag="PLH96_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPB60208.1"
                     /translation="MISTEQIKELRERTGISISQCKKALEEAGGDMTKALELLRAKGT
                     EVADKKAARALRAGVVACYSHNTGTIGAMVQLNAETDFVSNNSEFKVLADDLAMHIAA
                     MNPASVSELLEQAFVKEPSLTVDALVKQYIQKFGERIDVARFSRFDSAEAVAESGSDS
                     "
     gene            complement(19992..20753)
                     /gene="rpsB"
                     /locus_tag="PLH96_00085"
     CDS             complement(19992..20753)
                     /gene="rpsB"
                     /locus_tag="PLH96_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPB60209.1"
                     /translation="MQTEVKDAQKEGLIQELFKIGAHFGYSRSSRHASTKPFIFGFKN
                     KTAIIDLEKTIEMLEAAKEFAREVGRTKKILLLVGSKNEAKKIIKSIAESAGLPYVTE
                     RWIGGTLTNFKEIRKRVERMEDLRAKSEKGELSVYTKKERGMIAKELRDLEHYFDGIV
                     GLNRLPGAVFVIDADREEISVAEAKKVKTPVISLCNSDCNVSQIEYPIVANDSAITSI
                     SFFTKQIVEAYKEGLAAAPEEPKKEEDKIADKKEA"
     gene            complement(20798..21784)
                     /locus_tag="PLH96_00090"
     CDS             complement(20798..21784)
                     /locus_tag="PLH96_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPB60210.1"
                     /translation="MDDEIQIYRNNSKTTYLASLCDELTKQIEENKTLATDSEMAVLA
                     REEIKKLEKQRQELIDKMAEILSPKGEMGPGPKSLIMEIRAGAGGDEAALFALDLAEM
                     YGSYVKSRNWSFSKIDDSLSESGGYKEAVFEIAGAGAYDELKSEMGVHRVQRVPATEK
                     QGRVHTSTVSVAIMPVREKIDIKIDPNELEIEFTRSGGAGGQNVNKVETAVRMTHKPT
                     GIVVRCQNERTQLRNKEKALEILSAKFQSLHEEEEAKKLSVERKNQIGTADRSEKIRT
                     YNYLQDRITDHRIKKSWHGIDKILAGDLEGLLSDLKVSLGERLTREGDEPEK"
     gene            complement(21821..22069)
                     /gene="rpmE"
                     /locus_tag="PLH96_00095"
     CDS             complement(21821..22069)
                     /gene="rpmE"
                     /locus_tag="PLH96_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPB60211.1"
                     /translation="MKKDIHPKYFAKAEVTCACGNTFTIGSTVDKLRVEICSACHPFY
                     TGNEKVMDTAGMVEKFKGRMAKSKAAKAKKIAKADKEA"
     gene            complement(22200..23555)
                     /locus_tag="PLH96_00100"
     CDS             complement(22200..23555)
                     /locus_tag="PLH96_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HPB60212.1"
                     /translation="MSSNENLGRCPRAVSLIFICLLALVASQSAFYPTQAETEAEIAA
                     KEAKLKAELAKVEKEISEQKGLLSTKQKETASIQRDIDILNYKINTAQLNIKKKKLEI
                     ERLGGDISDKVEYIGELDEKVGKNKVSLAELLRATREIDENSFIELVMDKNNVSDLFV
                     EADRYYFIQQSMHKTLSSTRSTKNLTEQEKKQLEVRRAAETNAKKAIEVEKANIERLN
                     AEKQVYLNMSKTQEGAYRTIIAEKEKQRAAIRSALFRLRNVQAITFGEALELAKQVSA
                     ATGVRPAFILAIITQESNLGQNVGTCNRPGDPPEKSWRVVMKPERDHAPFLRITKSLG
                     LDTDTQPISCPYMGGWGGAMGPAQFIPSTWAAYESRIAKATGNNPPNPWNPKDAFAAS
                     ATYLSDLGASAGTFTAERTAALKYYAGGNWANPKNAFYGDQVMKLVATYEQQIKILQD
                     D"
     gene            complement(23624..24289)
                     /locus_tag="PLH96_00105"
     CDS             complement(23624..24289)
                     /locus_tag="PLH96_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPB60213.1"
                     /translation="MTIKLKAQKRDVFGKQLKKVREAGQVPVVVYGAKQEATPLVVDT
                     KEFVKVLKEAGESSIVSLDMEGKGHDVLIHEVQYHPIKGQPIHVDFYVVQKDKKIEVD
                     VPLVFEGVAPAVKELGGNLVKVMHELPIEVLPKDLPHDIKVDVSGLATFESQILVKDI
                     KLPSGVTVLAELEEVVVSISKAGEEVVVEEAPIDLSQIEVEKKGKQEEEGSEKASAEE
                     KSE"
     gene            complement(24394..24768)
                     /locus_tag="PLH96_00110"
     CDS             complement(24394..24768)
                     /locus_tag="PLH96_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60214.1"
                     /translation="MKVCLDCLDRRSLEIICCAIVRFEISTKTRIELVLDPDTANVFI
                     VSTSSKFLQPGNGKSGCLVVFPAGDRYLVTNKAGEYIFSLDHEEGDPNHLVRFFEWSE
                     FSVAVLDHTLERIKQMMKTTNV"
     gene            complement(24868..25734)
                     /locus_tag="PLH96_00115"
     CDS             complement(24868..25734)
                     /locus_tag="PLH96_00115"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPB60215.1"
                     /translation="MKTLRQIISEANETKTALGHFNISNLEGFWGVVRAAQNLDLPVI
                     IGLSEGERDFIGLRQARALVDSARTEFNQSIFLSADHTSSLKRAQEAIGAGLDLIVFD
                     RSELSPEDNIAETKKCVELARSVNPEILVEGELGYIGKSSKMLDELPVGVALDESSIT
                     KVEEAERFVKETGVDLFSPAVGNIHGMLKHAANPRLFIQRTKEIRDVTGVPLVLHGGS
                     GLTDEDFVAAIEAGVAIVHINTELRVAYKQALIESLQENPEEIAPYKYLKGPIIAVKQ
                     VVEKRLNLFSRR"
     gene            25771..26769
                     /locus_tag="PLH96_00120"
     CDS             25771..26769
                     /locus_tag="PLH96_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPB60216.1"
                     /translation="MDKFNLLTIGDTAIDAFVRLNEAELYCDINREECKICLKFASKV
                     PYESISEIPAGGNSANVAISGAKLDLTTAYFTNIGDDDNGQKILAKLKNANVDTSFIK
                     IHANLATNYNFVLWYADDRTILVRHENYPYELPTLPQSDWVYLTSLGEKSLTLHRELI
                     DHLDDNPETKLAFSPGTMQIRAGRETLAEIYQRTEIFFANKQEVMEILNTKEEEASQL
                     AKEIRNLGPKIVVITDGNQGAFALTDSGFWHSPIFPTENKLLERTGAGDAFASAFLAA
                     FNKGLTVPEALAWGAVNSASVVQKIGPHEGLLSAQEIEKRLAASVFEVKKIEEIKF"
     gene            complement(26824..27390)
                     /locus_tag="PLH96_00125"
     CDS             complement(26824..27390)
                     /locus_tag="PLH96_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPB60217.1"
                     /translation="MNKDNNKEIEVKLLVADFSGLAEKIKVAGGVLLQPEFFQRTVRF
                     DTPEQSLEANKTFLRVRTGDKNVVTLKRRIDGSELEARGLKEREEVETEVGDTEKVRQ
                     ILNILGFTREFIMEKYRTDYHLDETVISLDRLPFGNFVEVEGEPEEIETTIQKIGLTE
                     AERSARSYWGLFDEYKKKHNLSGENIVF"
     gene            complement(27377..28366)
                     /locus_tag="PLH96_00130"
     CDS             complement(27377..28366)
                     /locus_tag="PLH96_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPB60218.1"
                     /translation="MKILADKVFDLSVAQKAIRDGFGEGIMAAAEKDEQVVALSADLS
                     DSTRLDGFRRKFPSRFFQVGVAEQNLATVAAGLAVMGKVPFMTSFAVFSPGRNWEIIR
                     TAICLNEVGVKIVGSHAGLSVGPDGASHQALEDIALMRVLPKMTVVVPADAEEAKKAT
                     MATAEYRGPVYLRLSRVEAPIITSPEAPFEIGKALVVRESENPKVAIVACGLLVYRAL
                     IAAQKLAKEGIECEVLNNHTIKPLDKETILAMAKKAGAVVVAEEHQKTGGLGGAVAEL
                     LSENYPLPMEFVGVDDRFGQSGSSAELLEHYGLAIDDIIEAVRRVIKRKNEQG"
     gene            28457..29815
                     /locus_tag="PLH96_00135"
     CDS             28457..29815
                     /locus_tag="PLH96_00135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB60219.1"
                     /translation="MPITSILTGEIFKRKATVNQSRAFSLRKSSALILLGILITYGIF
                     ILKVQIFHSPPVLFLLLYSAAMTAFQFGRLLTVLFYEESLERIRGKYSANSLIGQDPY
                     EPSVSFVIPCMNEEKAIRHTINKCFEVDYPTHKVEVLVIDDGSTDKTPEILDQLNQEN
                     DRLTVIKFAQNRGKRYAMVEGFKRARGEIIVQLDSDSHLAQEGLRPLIEPFQNPSVGA
                     ACAHSDPANVDKNFLTKMQAAYYYMSFRILKAVESTFYTVFCCSGCASAYRQSAVLAV
                     LDEWSRENFLGLPVMYGDDRSLTTRVIKEGYKTIYTAEAQAYTIVPETFRQLIIQQVR
                     WKKSWIINAIKTGKFIITQKPFIAILYFYPLVILSLINPFVALYALVYVPIVYGIWPI
                     FYLAGIFLITALIAVYYKVYGPQNRYWPYLFAWGAFSVTVLAPLLFYAAARIQNRSWG
                     TR"
     gene            complement(30194..31882)
                     /locus_tag="PLH96_00140"
     CDS             complement(30194..31882)
                     /locus_tag="PLH96_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB60220.1"
                     /translation="MKSGQKGVSVNSGKTSLSVHMGGTLKNLARFYPDMASVVDEMIQ
                     NALDAGATLVQLRVNQKTRFIAIRDNGRGMSQQAFEKRVSQSIGHTLKSEEDLGQFGI
                     GAISAWGKCQEFSVTSCPAPRKEAYVTWTFPVDSEASGVSVDWRERKDLRFAPDQVNV
                     RSGQNVWWRTAIEIKEYVKDRALSEMTMKGILESATMKYSSKMERLGTRLRVDFVDYA
                     GNESSAEVAAAKFTGTRLPERRYYRESSGEVIVRMYLAKRHDSRGRVNIKFGEAGNDF
                     RFPFSHFRTSSAKELLDSRVMEALGSGIFEGEVLGELVQLTPSRTCFERNDAMIDLCV
                     SLETWFREEGEHYLDEQRRVRQSERYQEIGHDALSRLDRLFRESPRGEDLKAALSRFR
                     YGSIGLGHSERETTREQEEKSKAITGSQKKVGGEGSKRSQEEAIREHDEPHLTSRGPN
                     GSKRRLVQGGSHGLQFAYDEMPDSPKLWELDLDEGVIVFNTMHPDWVECDRKGESNWR
                     LCSLLVICAIQALSLLAMPDQAMWPHQRKYADTVTGHLASWLVMGGGMPLGRRA"
     gene            complement(31914..32837)
                     /locus_tag="PLH96_00145"
     CDS             complement(31914..32837)
                     /locus_tag="PLH96_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60221.1"
                     /translation="MVATLFLWKKASLSAWKVGRNMIVRPRLGRSCFWVMFESLREAG
                     EPSWLVLWFSSNKQKKELNMNLMECLKEFGVRASAKDIKLLAEFVGVSSLTVNGWLRD
                     VSRGRPMGEKALKVVHFLNLLGYHAEELDGLKGPIYDFGKAIAFGAISFEEAVQRLNY
                     KKPSHLLVVLRGESGILRGRQETMRKFCKSCETELERNLATWREKLQLPSREEGNSWS
                     SDQEGKSSTLVGGLDNQKRRDVIEGLARLLQACLPLAQLVGSDVFSANDREKLRELAG
                     RETVHQTSVALTKLAGERARERTLEREHRVL"
     gene            complement(33101..33934)
                     /locus_tag="PLH96_00150"
     CDS             complement(33101..33934)
                     /locus_tag="PLH96_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB60222.1"
                     /translation="MLLTDQKLEYLSDQARAVRRSVVEMLVTSGSGHTGGPLGLAEIF
                     AALYFGLLNHKPDKPDWPERDRLIVSNGHACPALYAAMARAGYFPSEELKSLRRYGSR
                     LQGHPERLKLPGLETSSGPLGEGLGQALGMVLADRLDKGRGSQRFFYCLLSDGELQEG
                     STWEAVMLAGKEAPHNLIAIVDRNHIQIDGVTEEVMPLEPLADKWRAFGWQVQEIDGH
                     SFPEIFNAVGQAKAVFGRPSVIIAHTIPGKGVSGWERKYEWHGKTPNAKEAERALKEI
                     E"
     gene            complement(34022..34648)
                     /locus_tag="PLH96_00155"
     CDS             complement(34022..34648)
                     /locus_tag="PLH96_00155"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB60223.1"
                     /translation="MNIIPSIIASDFAEVKEKLAKLEGLVEWVELDVMDGVFVPNVSW
                     QAEEDLREIDGQIKLSAHLMMGNPETVVEDWQTLVDRIIIHVESTDQLDSLLSRFDNG
                     LNPCEIGLALELETSLEKVKPYLNKVKIFQLMSIANIGYQGEKFDARVLPKIKELREL
                     ASDAKIIVDGGIKPEMVAELSEAGVDALVVGSYIWRSKNIEEALRTLS"
     gene            complement(34751..35221)
                     /locus_tag="PLH96_00160"
     CDS             complement(34751..35221)
                     /locus_tag="PLH96_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPB60224.1"
                     /translation="MKIYLAADHAGFALKEQIKNHLKERGLVVEDQGAYALEPEDDYT
                     DYVKIVAKLVQGKSGSRGIILGGSGQGEAICANRFAGVRAAVYYGGSPDIVELSRAHN
                     DANILSLGARFVSPGEAIQAVDLWLSTDFSGEERHLRRINGIDDMDNVGGALKY"
     gene            complement(35365..36381)
                     /gene="gap"
                     /locus_tag="PLH96_00165"
     CDS             complement(35365..36381)
                     /gene="gap"
                     /locus_tag="PLH96_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB60225.1"
                     /translation="MMVKVAINGLGRIGRAFFKLAVKNPEINLVAVNDLGDPANLAYL
                     LKYDSAYGQFDGEIKFEDGKLMTAGKSARFFQEKEPTNLPWAELGVDVVLESTGVFEA
                     YGKSKAHLDAGARKVVISAPVKDEPAPGIPGGTFLIGINDDKIADQVITSNGSCTTNC
                     AAPVIKILDEAIGVEKALLNTTHGYTATQKLVDVADAKDWRRGRAGAINLIPSTTGAA
                     IAVGQVIPEMAGKFDGVAVRVPVITGSMIDVTFVAKKNTTVAEVNAAFEKAANNPRWQ
                     KTFTVTKEPIVSSDILGSKYASIADLSWTKVVGGNLVKVMAWYDNEMGYAHTLVEHVL
                     KAGR"
     gene            complement(36495..36956)
                     /locus_tag="PLH96_00170"
     CDS             complement(36495..36956)
                     /locus_tag="PLH96_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60226.1"
                     /translation="MDKKFSWLGLAYFLFIVGFPFVTWGGWGFVFFLVFILPILTVLT
                     IITLIKYHKGDQKTGRKWLLLLGGVYAAACLLIIDGADFGDAQGIERLFGHLFRGPVN
                     FIPESIGENLAGVSMFLYLLFFALNIAFITSKPRAKEINKNSTSTSAVVNR"
     gene            complement(37056..38486)
                     /gene="gatB"
                     /locus_tag="PLH96_00175"
     CDS             complement(37056..38486)
                     /gene="gatB"
                     /locus_tag="PLH96_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB60227.1"
                     /translation="MSEIKYKPTIGLEVHVELNTKTKMFCRCLNNPDERQPNVNICPV
                     CTAQPGALPVINKDAIWHILRVGVALDGRLADFTEFDRKNYFYPDIPKGYQISQDKYP
                     LVSGGSLRGVAITRVHLEEDTAKSFHDVKTGESLVDFNRSSLPLMELVTEPVINDGPT
                     ASAFAKELQLLLRYLGASEANMEKGQMRIEANVSVAPEGDKLGTKVEIKNLNSFRTVE
                     KAIDEEIKRQTGVLEAGGRVIQETRGWDENKQTTFSQRLKESSHDYRYFPDPDLPKLF
                     ISEIPEFRNVVESLPELPWQRRRRYAELFGLKTEDVEVFVQDRSLAGFFDELTEGLDH
                     ALDETKEYLKVAANYITSDILGLAALAGELRLPPVGDYAELLKMIMGGEVSSRGAKDI
                     LAIIIKEGGEPRQIAKERGLFQVNDEDAMSVIVDKVIVDNKNVFEEYKAGKEKALQFL
                     VGQAMKESKGSANPGLLKKLFEQKKG"
     gene            complement(38591..39505)
                     /locus_tag="PLH96_00180"
     CDS             complement(38591..39505)
                     /locus_tag="PLH96_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPB60228.1"
                     /translation="MKKIIFSILAVLGALVLVAVVGCLVVITSLSGARVAVTSFQNSE
                     AVLSPDSADYYQEADTKSATTEVLPQRKVSRNGSMSVIVEKAEEAVETITNIAEQAGG
                     FVSVARIYEIASDIKSGHITIKVPSDKFDETIDKIKDATVKVEREEVSTQDVTDQYVD
                     LETRLRNYNATEDKYLEVLKKAQTVNEILNVQRELSQIRNNIERIEGQIKYMDRQVEM
                     STISVSLTSEADIEVFGVRWRPLTQIKQSLRTLVKGSVKYVNLMIAFIALLPLIVLWL
                     ITIAIVLLIIWKVGRWAKNRFFPRKIGQ"
     gene            complement(39575..>40048)
                     /locus_tag="PLH96_00185"
     CDS             complement(39575..>40048)
                     /locus_tag="PLH96_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60229.1"
                     /translation="RRLGKSDCRFGYRDSVFKKRNWLVLRVCLELKKGDKLALHKVAR
                     NLIIVRDNKYNGLLCAGSFFKNVPLSRISKTSLKKIDPTKIFNKKVSVGYLIEASGGK
                     GLSAGKVAVADFHANFLINRGGGATRDLKKLAEKIKRLVKQKFGIALEEEVRLLS"
BASE COUNT        10933 a        10369 c         7808 g        10938 t
ORIGIN      
        1 ctacgagacg tggggttttt agtccttgca ttaaaatatt tatctctcta catcaagact
       61 tataaataaa cttataaaat aatctgttat acttctaaaa tgtcttctcg ttccccaaaa
      121 acagttaaaa aacattctca aaaacccaaa aaacctctga ctggtgtcat ccgcgtgaca
      181 gcgggtgctt ttggcttcat cgaccaaggg cgccggccaa caccgacaaa accctcctct
      241 gccaaatctg ccaaaggttt tagcaaaacc caacgcaaat ctgggcccaa aaaaaacgag
      301 acggttttta tcccaccggg agctttaaac accgcccttg acggcgacga ggtagaatac
      361 gttatgaccc gcggcgacaa cggcgaagta ttaaaggtcc ttaaacgcgc ccgaacagaa
      421 ttcgtcagcc aagtcaccgg ccgagaaaac ggttttttaa ttgttaaacc tgacaacccc
      481 aaattttata ccgacctggt catagaagaa acagccgaaa ctgccgcgct ttccatcggc
      541 gccaaaattg tggctaggct caaactttgg cccaagagca ccctccgccc tggtggcgat
      601 tttatcaaaa atttgggtca aagcggcgaa catgaaaccg agatgcaagc gatcatccaa
      661 gaccgcggtt taattgaagg tttttccgtt gagctggaaa cagaggcgca aaaaatcgcc
      721 caagaacaca atttggacgc ggaaataaaa aaacgaaaag atttccgcga ggttacaact
      781 tttaccattg accccttggc ggccaaagat tttgatgatg ctctgtccat ccgccaaaca
      841 gcagacggct tatgggaagt cggaattcac attgccgatg tgtcgcatta cctaacgcct
      901 ggttctttgc ttgatttgga agcgcaaaaa cgcgccacct caatttattt ggtggaccgc
      961 acaattccga tgttgcccaa aattttatca gacgaccttt gcagtcttgt cccccatgaa
     1021 aatcgtttaa ctttttcagc agtttttgaa atggacgaca aagccaacat caaaagcgag
     1081 tggtttggtc gcactgttat caagtctgac cgtcgtttca cctacgagga agctcacaaa
     1141 atcattgaga ccaaccaagg ggaatacgca gccgagttgc gcaaactaga tagcctagcc
     1201 aaagaactgg accgtcaaaa aattagcgcc ggcgcgttgg cctttgaaga agacgaagtc
     1261 agttttgaga tagatgctct cggtaagccg ctcaatgttt ttaaacgaga acgcttaggc
     1321 acgcacaagt tagttgaaga ttttatgctc ttggccaaca aaaaagtggc cgagtatgtt
     1381 tctcggctag ccaaaaatca aaacggcgtt tttgtttatc gtgttcacga cgcgccagac
     1441 ccggacaaat tggatggagt cagacgcttt atcaaccttt ttggccacaa attgacccac
     1501 aaaggcagtc gtcccggccc gcaagagata aacaatttct tgaaaaaaat tgccggccag
     1561 cctgaagaaa gtgtaattac gcgcgagatt atccgctcta tgagccgcgc tgtttactct
     1621 accaaaaata tcgggcactt tggcctggcc ttcgcgcatt acactcattt tacttcgcca
     1681 atcagacgct atcctgatgt catggttcat cgtttgcttg atacctatct caagggacag
     1741 tcgccagcca aagatttgct tttgcgttac gacaaattgt gcgaccactc ttcaagtatg
     1801 gaaaaactgg ccaccgacgc cgaacgcgag tccgtcaaat acaaacaagc ggaatatttg
     1861 gaaaatcatc tcggcgaggt ctttgatggc gttatctcca gcctaaccaa gtggggcatg
     1921 tatgtggaag aactctcgac tggcgccgaa ggtttgattc gctatcgcga gatggccggt
     1981 gatcgattca tctttgaaga gaaaaaattc caggttcgcg gcgaaaagac aaaccaaatt
     2041 tatcgtttgg gcggcttagt caaaatcaaa gtagccaaga ccgaccgcgt gaccaaaacg
     2101 attgatttca ccctagacac agattgacac aaataaaacg ccgattttta aattaaataa
     2161 ttattaaagc aagacaaaaa agaagccggc attttggttt taaccaaaat gccggcttct
     2221 taaaattttg aattttaatc tttacatttt acacttctgt cattttgcat attgttccgc
     2281 ttccgtcagt ttgagcgaaa tcagacgcga gactgaatca gaacccatcg tcacgccata
     2341 aagcactccc gcgcgggaca tcgtctcgcg gttatgcgta atcaaaatta gttgcgagaa
     2401 ttgtgacaag ttttcaatca tatcgccata cttgcgcgag ttagcttcgt caagcgcggc
     2461 atccgtttca tctaaaatca taaagggtgg cgggttaacc tgcgacatcg caaacagaag
     2521 cgcgatagag gttaaggccc gttcgccgcc agataacatc gccaagcctt tgattttttt
     2581 gcggggcaaa ctgacaatga tttcaattcc ctcctcggtc ggttcatctg aatcctccat
     2641 tgtttctccc agcggagcca agtcgtcact gcttgccaaa tcaaggcgcg aacgtctttt
     2701 ttccgggata accaaggaaa gcgaagcgga accgccgcca aataaaattt caaaaaactt
     2761 ttgaaattcg ctgttaattt ttttaacacc ggccttgaat tcgtcactta tcctctggcg
     2821 taattcggac atcacttgtt ccaacgagtc ggcgcttgtg gccaaatcca acaattcctt
     2881 ttccaggtaa ccatcacgcg ccagcacctc gtcatattcc ttttccacat cgacgccctc
     2941 gccgccaatg tcctctaagc gaatcttcaa gcgttccaac cggcgatgac gctcttcttg
     3001 ggttgctcgt ttttccgaca acgcctcttg ttcatccaaa ggatagtcct cataattgag
     3061 ggcttcgcga ccaatcaaga cacctgcctc acccaattcg cgtttgaaat tttcttcttc
     3121 cacggccaaa gtctctagct tgccttccag cgttctaaca acagccgcta attccccccg
     3181 tctagcccgc aattcataag attcgcgctc ttcttcccgt tcttgatttt ctctttgttc
     3241 caaccgctgt ttggccaatt ggtattcttc ttgacggctt tgttccaacc tttccagctc
     3301 agccaaagcc ttttccttct cctgtttttc aaccaacaat tctttgatca tttccaaatt
     3361 atcagtcggc gccaattgat gaagtttaaa cccaccccaa aattcaccca aaatttgccg
     3421 gatgttttgt agaatccttt tcaaaactgc cggattattt tccgtcttag ctttttgcca
     3481 ttctccttcc aactcagctt ccaacctttt taagtcatca cgcgaaaccg ttaatttgtc
     3541 tactgttggc gtggccgaac ggctttctcg ttgtttcagt tcaatcatcc cttccaagcg
     3601 gcccaaacgg cgagacaaat cgtctttttt ggcttgccct tctcgcaagg tcgcttctat
     3661 ttctcgcaat ttatttttct cctccaccgc ctcgtcagga gcagaaacct ccttcgcggc
     3721 caaggctgat aatttttctt ccgtggctaa caaaatttcc ttgggaattg ccaattcttt
     3781 tttcaatttg tcacgagtat aggcaagata aaatccttct cgcttcaagt attccgaata
     3841 gaatgtagcg agttcggcgc gcagttcttt agctttttcc actttttcca tttgtttctt
     3901 caaaaatttc aaatggggcg caatttcccg acgaagaatc tccgcctctt tgatattctc
     3961 ccgcgtcttg gtcaatttct tttcgctttc ttccaatttc cattggtaaa ttttcaaacc
     4021 aagagcgtct tctatcattg cccgcctttc aaccagattg gcatttaaga tacgatcagc
     4081 ctcgccctgg ctaatgatgt gatgaccgga agaccccaat gaaatccccg ctaaaagggc
     4141 caagatatct tttaatctga ctttgctgtt gttaatggag tattcgttcg tgccatcacg
     4201 aaaaacttca cgcgtgatat tgacctcgtc aaaatcaatg ccagcaaaag cccgctttga
     4261 gttatcaaaa gtgaccgaga cactagctcg actcagacgg cccagcgatg atgagccatt
     4321 aaaaataagg tcctcgcctt ttttaccccg caaagacttg agcgactgtt cacccaaaac
     4381 ccaacgaaaa gcctccgcga cattggattt gccggaaccg ttcggcccaa ccaccgccgt
     4441 aatcggcgcg tcaaagagca agaccgcttt acggccaaaa gatttgaaac cattgatctc
     4501 cagacgtttt aaagtcatca cctaaaatta aaagtcaaaa ataaattaaa aaacaaaatc
     4561 taaaactcaa caccgatggt catattcgtt caccagccct ttttcgccaa gcctgatttg
     4621 gcggcttctt gttcggcctc ttgtttggag agaccttcgc caactgccac ttgctcttca
     4681 ccgataaaaa gcgcgattgt aaaaactttg ttgtggtcag ggccaatttc tttcagcaac
     4741 tgataggacg gcgtaatccc atacacttcc tgcgcctttt tttgaaaata acttttatag
     4801 tcttcaacaa tcgtcttctt gataatttct tcagcccggt ctaagagata cttttgaata
     4861 aaattaaaag ccgcctcgta gcctcggtcc aaataaatcg cgccgataat cgcctcaaag
     4921 gtattggcca aaataatctg acgcgcccga ccggtatctt tggcctcgcc ctttgacaaa
     4981 aacaaaagct cgttcacccc aagttcaagc gctaatgccg aacaggtgtt ggcgttaacc
     5041 aaggccgccc ggtaagaagt aagttctcct tccggcttgt tgggatattt tttaaaaaga
     5101 aaatcagttg ttgccagttc caaaaccgcg tcacccaaaa actccaaacg ctcattatga
     5161 tcaaaaacaa agccccgatg ttcattcaag taagaacggt gaacaaaagc ctgtttcaat
     5221 agttttttgt cagcaaaatc caaaccgata tttttttcaa attcagacaa actcttgttc
     5281 attttttact ttttcagcta aaattttaat cgctttggtg ataatcggca caatatcatc
     5341 atatatcttt aattccggca ctttggcgaa agtaaaccaa ctggcgccat ctaaaccgcc
     5401 tttgaccgcc aactttattt cccccggttc acatttggct aaataatatt taacttgacg
     5461 tctagttttc cccttctcgg ggtcagaggc gatgtattca ttggagccaa gttcttgacc
     5521 aatgacgaca tccaacccaa gttcttcttt ggcctctctt tttatgccct ctttgacact
     5581 ttcaccttct tcaatatgcc ccttggtcaa agtccaaaaa ccaaaaacat catgaacaaa
     5641 agccagttta atctcgcctt tatcctcgca aaaaaccacc gcgccaccaa gtttttctat
     5701 cggcatttcc tcataaggaa tgtctttgat ttttttcttt ttggctggcg tgtcatcttt
     5761 ccccggttcg cccatctcct tgtagaccgt ccctagaacg ccatttacaa agcggccact
     5821 attttcaccg ccaaaagttt tggctaattc aatggcttca ttgatggcca ccttggccgg
     5881 cacatcatca cggtcgccaa aaatcaactc gtaaagaccg agacgcaaaa cgttgcggtc
     5941 aactgtcgcg atttgttcaa ggggccaatc cggcgccgct ttttcaatga ttttatctat
     6001 ttgcgcgcgg tgtttgatta ctccgctggc aatttctttt ataaaatttt tttcttccag
     6061 accgggcgcg aattcagcca caattctgtc tattgtttcg tccaagtctt ttattttgcc
     6121 gtcaccaaag tcccactcaa aaagcgcttg cattgccaga cttcttgaaa gatgacgatt
     6181 agccatagat gattttcaaa tattcaaact aagcctaccc acttgggcag ttcgtaaatt
     6241 tatttggcgg ccgccttttt ctttttggcc tcctttttct ctgtcttagc caacacgttg
     6301 accacttcgc gcccccgata agagccgcaa ttagggcaaa cacgatggct agccttggct
     6361 tcgccgcaat tggcgcactt tgaaagacgc ggcgctttga gcgcgtgatg agaacgtcta
     6421 tttccggtat gagcccgggt atgtcgcatt ctaactacca tgaattaaac tatagcttaa
     6481 atgaattaaa aaagcaatat gcgacgccaa taagctaatg tttcgtttta atttccaata
     6541 ttaaatatta aaagggccga acgcttcgcg attgcgtcgg cccctctggc tgtgcccctg
     6601 gtgaaagtct ggtgaaagtt atgcgacctg cgtccggata gcccggacac agtttcgtct
     6661 gaccgcgtcc cgatacttca ggtccacgta ctggatctgc ccgagcagac ggcgagcgga
     6721 ttcaggcgag aggttcttgc ggctcaacac cgagaacccc acgtaagaga gctggggtga
     6781 cataaggacc atcacccaca actccatatc gctcagcaag agttcaacga caccttcggt
     6841 cttccccttg gcctcttgag ccaagaggac gatggccgac ttcaggccga ccctctcaac
     6901 caagatgtca aaggttgggc gggcgatctc cccttgttgg aaaagccgca cgaccctctt
     6961 ggcaagagcc aaattgattt cttggctctt tgttgtccct tccagtctcc ccttttgcaa
     7021 cttcattctt ttcccctttt gttttacaca gttttacaca gccagttcca tcttcattta
     7081 taccacaatt cttaaaacta agcaaagtaa gagcgacgat ggcaggcaga caagcccagc
     7141 tccgccactt tggcaaaatg ctctttggta ccatagcctt tgtgtttttc taaaccatac
     7201 ccggcaaatt tggtggccat ttttgtcatt tttttatcgc gcgagacttt ggctaagata
     7261 gaagcaaaag aaataatcgg ctccgtttca tcgcccttga taattgtctc ttggtaaacg
     7321 aatttttcgg gcgccttcaa gcttccatcc aattttaaaa agtattctat cggctcgccc
     7381 ggcagttttt ccaaacaagc ctccagccct ctcgcgaccg ccggaacaat ccccgcccgg
     7441 tcaatataag tttccgcgac tagagaaact tggcagaggg cgccggtttg tttagttagg
     7501 ttagtcaaaa aagcaaacca tttttcgcgc atcctcggtg ttagcttttt ggagtcgcgt
     7561 ccgaccgggt aatccggcgt agcggacagc aagtccccaa cctcaagcgg ccaaacaaac
     7621 atccccacag cgaccggtcc agccaacgct ccgcgaccag cctcatcaat gccaaccagg
     7681 tattttaccc ctttcggaat ttccattctc ccattctaac tcaatctcaa ggctattaaa
     7741 aatatttaaa aacaacttat gttttatcca aaaaatcgca cgacatcctt cctttttccg
     7801 caagtggcaa gaaccgacta ggcttgttaa aattaactca atgtttgttc atctccacaa
     7861 ccacactcat tacagccttt tagatgggtt aatcaaaatt cccgacttgg tcaaaaaagc
     7921 taaagaatac aatatgccgg ctttagccat caccgaccac ggtaatatgt atggcgctgt
     7981 tgaattttat caagagtgcc aaaaacaaga catcaaaccg attatcgggg tggaggttta
     8041 tgtcgccgcc agaagtcgtt ttgacaaaga accccgaata gactctcgcc gttatcatct
     8101 gactcttttg gccaaaaacg aaaccggcta tcgcaattta atcaagctgg tcactaaggc
     8161 taacctggaa ggtttctact ataaaccgcg agtggactgg gaactactgc aagatcacac
     8221 caaaggcgtc atttgcctgt caggttgtat gggcggtcaa ttgtctcgac tgttgttaaa
     8281 caaagaccac gccggcgccg agaaactggc cagggattat gccgaagcat tcggtccaga
     8341 taattattac attgaaataa tgcaccaccc caaaattgag gggcaaattg aattggccaa
     8401 aaacttgatt gctctagcgc gcaaactaaa cctaccgtta gtggccaccc acgacaccca
     8461 ttacttgaac aaagacgact cgcgcgccca ccacactctg ctggccattc aaaccggcaa
     8521 cgatgttgat gatgtcaaag gggtaaccaa tatggaagaa gatttttcct ttgtttcagc
     8581 ggaagaaatg gctagcttct ttcccgatac gccggacgcc ctggagaaca ctttaaaaat
     8641 ttctgaggcc tgcaatctgg aactcgtttt gggcaaattt gtttttccca actttgaatt
     8701 agacgacgac aaaacagctg atgaggtttt gcgacaactg gctttttccg gcttagccaa
     8761 acgcggcttg gataaagacg aagcggccat cgctcgcctc aaccacgagc tggaagttat
     8821 caagataaaa ggttacgctt catacttttt ggtggtagaa gacctgattc gtttcgcgcg
     8881 cgaaaataat atctattaca acatcagggg gtcggtcgcc ggttctctta ccacttattc
     8941 tttggatatc accaaagtta acccgctcgc ctatcacatt ccttttgaac gtttcttgaa
     9001 tccagaacgg ccttcggcgc ctgatattga tatggacttt gccgacaata agcgcgacaa
     9061 aattattgac tacgccaaac aaaaatacgg cgaagaaaaa gtcgcccaaa ttggtacctt
     9121 tggcacgatg atggcgcgtg gttccgttcg cgacgtggcg cgcgcgcttg gtttccccta
     9181 ccaagccggt gaccaaatcg ccaaattgat tcccatgggt tcacagggtt tcccaatgac
     9241 tattgaccgg gcgcttgaga tggtaccgga gttaaaggaa atgtataacg aacgcgccga
     9301 caccaaagag ataattgata tggccaaaaa aattgagggt tgcgctcgtc acatctccgt
     9361 tcacgctgcc ggtgtagtaa tagcgccggc tcctttggac caatatgtcc cgctccaata
     9421 tgaccccaag ggcgtaagcg tcatcaccca atatgatatg tactcagtcg gcgaggacgg
     9481 tgtcgggttg actaaatttg attttctggg tttgcggaac ctggcgattc tttccagcgc
     9541 ggtggaaatt attgaaaccg tttacaaccg acgagtggat atggatggta tcccgatgga
     9601 tgacaagaaa acttttgaga tgctggcgcg cggcgagaca gaaggtcttt ttcagctaaa
     9661 tggtagtggt atgaccaagt ggttaaagga actgcgccca actaccatcc acgatatcaa
     9721 cgccatggtt gccctttatc gtcccggtcc gatgcaattc attcctgact atatcgcccg
     9781 caaacataac ccacggctga ttaaatattt ggacccttcc ctggaaagta tcctggagca
     9841 gtcttatggt gttttggttt atcaagacga cctccttatt atggcaaata aactagccgg
     9901 ttattcttgg ggcgaagtag acaaattccg caaagcagtt ggcaaaaaaa ttcccgagct
     9961 aatggccaaa caacgcgaaa agtttatcaa gggttgcatt gaacattccg gttggaaaaa
    10021 agaaaaagcg gaagaagttt gggcttggtt ggaaccgttt gccgcttacg gctttaataa
    10081 agcgcactcc gccagttatg gccgagtcgc ttatcaaacc gcttatctca aggcaaatta
    10141 tccatccgaa tatatgacag cggttctgtc ggccgaatcg ggcgacgtag aaaaagtcgc
    10201 cgtcatcatt ggcgagtgca aacggatgaa catccccgtc ttaccgccgg atatcaacga
    10261 aagctttggc gacttcactg ttatcaaaaa cgcccaagac ccaaacttaa gcgaaaaagc
    10321 caaaaaatta gccaacaaaa accctgaccc ggttgataaa atccggttcg gtctttttac
    10381 aattaaaaat ttgggcgcgg aaatcgcgca attcattgtt gacgaacgta aaaaaaatgg
    10441 cccctatcga gacttgggcg acttcttaac gcgcgttaaa cacaaaaatt taaataaaaa
    10501 attccttgaa gccctagcca aagccggcgc cctagacagc ctgggcgaaa gccgcgggaa
    10561 gtttcttgcc aacttggaag agatcctcgc cttcaaccgc gaagaagcca gaaacaatgc
    10621 caaccaaact tcactctttg gcctgatgtc tgacgacgcg ccgaaaccaa ccttaaaact
    10681 acgagatgcc gacccgattg agccacgtct aaaattagcc tgggaaaaag aattgctggg
    10741 gctttacgta tcaggccacc cgcttgacgc gcaccgtgaa aaatttaaca aactggaaaa
    10801 tacgattggc caccaaaaaa ataaaagcga aggttttccg atcgtgactg gcggtattat
    10861 cggtgaagtg cgcgctattt ttactaaaaa taacaagcag atggctttct tcaaactgtc
    10921 agatttcaac gatatggttg agtgcgtcgc cttttccgat gtttacgaaa aatataaagc
    10981 tttgctagtt gaagataagt gcatagcgct aaaaggcaaa ctaaattatc gcaacggcga
    11041 accgagcgta ctggtagagg ccatcaaaga actatgagac gcagatttaa tgcagataga
    11101 ccgcaaatat ttcgcccacc ttcttgaggt agactgcaaa ctatcttggt gctatactcg
    11161 gatcattgaa ggttgcggtg actaccaacc accgacccgc cttgggcggg ttcctgagct
    11221 tgtcaaagga ttacaatcct gagcgaagcc gaaggattac aatcctgagc gaagccgaag
    11281 gactaattca ataaaaaatt cgacaaaatg agattgtccc ttggtaatcc ttcttcgctg
    11341 aaactacgaa ggattgtcct ccggataaaa atcgggtgga accgcgtgag tttaaataaa
    11401 aaattcccgc cccgacaaaa acgccaaagc gttttcgtcg gggcggtttt ggttaaacat
    11461 taacaactaa ttaataaaaa atggaaaaca ttgaacaaat cagacattcc ttggctcacc
    11521 tcttggccat ggtcgtcaaa gaaaaatacc cggcagccaa attgggttta gggccggcca
    11581 ttgaaaatgg tttttattac gattttgacc ttggtgaaaa aagtttaact gatactgatt
    11641 tggacgaact tgaaaaaaag atgcgttctt taatcagtca aaacttagat tttgcgactg
    11701 aaacaatcac tgccgacaag gcgtgcgaaa tttttgccgc tgaaccatac aaactggaat
    11761 tgattgacga actagagaaa aacagtgagt caatttctat ctaccgctcg ggagattttg
    11821 ttgatctgtg cgccgggcct catgtcgcca actcgtctga cattccccgc gattctttcc
    11881 gcctggaccg cttagctggc gcttactggc gaggcaacga aaacaataaa atgctcaccc
    11941 gcgtttatgg tctagccttt accagcaaac cggaactaaa tgattttcta gacaaacgag
    12001 ccgaggctga aaaaagagac caccgcaagt tgggcaagga acttgatttg ttttgcttct
    12061 ctgatttagt cggtccaggc ctacctctct ttactcctaa aggcaccatc attaaagaag
    12121 agttacaaaa agaagttgag tcaatttgtc gacactacgg ctttgaaaaa gtttccgctc
    12181 cctcattagc caagcaagaa ttatttgaaa tttccggcca tgctaaaaag tttggcgaag
    12241 aattatttca cgttacttcc gagaaaaaac atgatcttgt cttaaaaccg gttcaatgcc
    12301 ctcaccatac ccaaatatat gcctccaaga gtcgatctta taaggacttg ccgattcgct
    12361 acatggaatc agacaagcaa taccgtgccg aaaagactgg cgaagtaagt ggtctgtccc
    12421 gcgtttatgc tatcaccgtt gaagatggac attctttctg tcgtgttgat caagtcaaag
    12481 aggaaatcaa aaatatggtt tccattattc gagattttta cagtcgtctt ggtttatggg
    12541 atgagcactg ggtttctttg tcggttagag attatgaaca cccagaaaaa tatattggcg
    12601 aaccaaaaga ttgggatact tgtgaacaaa tcctccaaga agtatccaat gaaatgggac
    12661 tggaggccaa acgttgcgaa ggtgaagccg ctctttatgg acctaaacta gactttatgt
    12721 tcaaagacgc gactggtcgc gaaattcaga ttcccactgt ccaaatcgac tttgctacac
    12781 ctaaacgctt taacttggaa ttcactaacg agcaaggcga aaaagtcccc ccggtaatgg
    12841 tccatcgcgc tatcctaggt tcttatgaaa gattcttggt tctcttgatt gaacattacg
    12901 ccggcgcctt cccactttgg ctctcaccgg tccaagccaa aattttacca atctcagaaa
    12961 aacaaatggt ctacgccaaa gaaatcttgg cagaattaaa agccagaaat atccgcgttg
    13021 aactagatga aaataatgaa accttaggca aaaaaattcg taatgccaaa atggaaaaaa
    13081 ttccctactt attggtggtg ggcgacaagg aaatggagag taaaactgtg acagtggagg
    13141 cccgcggcgc caataagggc gagtctgtca gtttagccgt gttttgtgct aaaatagaag
    13201 aagggcttaa aagtcgaaaa taagctttaa aattaagaat tttttattaa aaatatgtta
    13261 ccctacaaga agattttggt cttggcttta atcttgaccg ctgcctggtc agctggttct
    13321 gttttggccg aagaagccac ctccaccgag gacaccgctt cctcaaccga acagacggtt
    13381 gaaacaaccg acttggatga gttaatcaat gagttccaag tcctgcgcgc taaattactc
    13441 aaagccttga ttgccgaatt taacgccaac aatctgaatg aaaaacaagt tcagttgtta
    13501 caagttttac tcgcctccga tttctccatc tatccagaag gagtgatcac cggcaaagtt
    13561 ggggacttca ccaaggccgc cgtcaggcgc tacatggaaa gtcagggttg gaacgttccg
    13621 gctcgcctct tgcaagctgg ggccggcaag tcaggcaata ttccgacggg cttgaaaacc
    13681 gcgccaggca ttcaaaagaa actgcaaatc gccagcggca ctcccaccac cacgccagtc
    13741 caacgtaatt tcctctacag actaaaaaac atcttccgtt aagaacaaat tccaaaacaa
    13801 aaccggccag aatggccggt tttgttttgg aatttgttct tcctttagat atctaggtta
    13861 gcggtcttag cgtgagtttg gataaagagc ttgcgaggcc cgacttcgtt gcccatcaaa
    13921 gtatcaaaag tcttatcagc atcatcaaaa tcgtgaattg cgacttgttt cagaacgcgg
    13981 gtctccggat tcagagttgt ttcccaaagc tcttccggat tcatttcgcc caaaccctta
    14041 taacgttgaa tggaaatctt ggcagttcgg gttttagcgc ttgattcttc tttatcctcc
    14101 tcgccctcta ctgccatctc gggctctacc tcttccgctt ccccttcctc aacttcccct
    14161 gttaagcccc ccagttcttt gattattttg tttttttctt cgtcattgta ggcatagaaa
    14221 aattctctgc cttttttaat cttatacagc ggcggttgcg cgataaaaat tttaccttgt
    14281 tcaatcagct cgcggtaata acggaaaaag aaagtcaaca gcaaagtcgc gatgtgcgcg
    14341 ccatcaacgt cggcatcggt cgcgataata attttatggt aacgcaattt ggttaaatcc
    14401 atcatgtcgc caatgcccac gcccatcgcg ataatcaaag aacggatttc ggcaaaagcc
    14461 atcattctgt ccaaacgcgc cttctccacg ttcaaaattt tacccttaag cggcagtacc
    14521 gcctgaattc gccgatcgcg accctgtttg ccggaaccgc cggccgagtc tccctctaca
    14581 atgaaaattt ccgattcttc cgggtcacga gactgacaat cagcgagctt gccaggcaaa
    14641 gtcattccct ccaaagcgcc tttacggatt attgaatcct tggcagcctt ggccgccttg
    14701 cgcgccttaa gcgccaaaat acccttattg ataatcgcct tggcatcgtc aggatgttcc
    14761 tccaagaaag ctgaaaacat ttcgctaaaa acggcatcca ccgcgctcct ggcttctacg
    14821 ctgcccaatt ttgttttagt ttgaccttca aattgcgggt tgagtaattt aaccgaaatc
    14881 acagccacca agccttctcg cacgtcgtca ccggtcaaac cgccatcgct atccttaagg
    14941 acattattct tcttggcgta atcattcaaa agacgcgtta aagcggtacg aaaaccggtc
    15001 acgtgcattc ctccctcagg ggtaacggta ttgttggtgt aactaatttc tttgacatct
    15061 atatcatcaa catattgcaa ggcaatttcc acaaaaacac cattttgttc tttgtcggca
    15121 taaaaaatct cccgatgcaa aggtttttct ttttgattat aaaatttgat taatgacaaa
    15181 agcccgcctt caaagaaaaa agtttttgac ggcaccggca aacctaagtc gcgtaaccag
    15241 aaaattttgt ccaaatcaat tttgccctca tacccacgcg cgtcaatcaa acgaattcgc
    15301 aaacttttga ccaaataagc ctgaccacgc agatggttga ccaaccggtc ccaagagtac
    15361 ttagtttcgg aaaaaatttg ttcatccggc ttgaacgtga taatcgtacc atgtaacttg
    15421 gaggcagaga ctcttttaac attatacttc tttttgccgc gctcatattc ttggacgtat
    15481 ttaccgtcgt tgcggtgaat ttccgccctg agccattctg ataaagcgtt gacaactgaa
    15541 gcaccgacgc catgcaagcc accagaaaac ttgtaaccac catcgccgaa ttttccacca
    15601 gcatgcaaaa tagtcaaaac tgtttccaga gccgagacct tggtcttctt gtgaatatca
    15661 accggaatcc ccgcgccgtt gtcaacgatt cgcaccacgt cgccgggtaa aagcgcaact
    15721 tcaatctcgt cgcacgagcc attcatcgct tcatcacgag aattgtcaaa aatttcaacc
    15781 aacaaatggt gaagcccatc aacaccagtt gaaccaatat acatacccgg tcgcttgcgc
    15841 accggctcca gcccctccaa aacgacgatc tcttcagcgt cgtatttacg attttttagc
    15901 tcttttttga ccataattaa aaattaaagc agcccgctta gggtccccgc cttgtatcca
    15961 ttatagcaca cccccacggc aaataacagc ccaaacgacg ctccaaaact gtttataatt
    16021 aagcccaatc cttgtttttg gtcattactt tctaacctca aaatcggcct tttccagagc
    16081 tccaatcacc ccttctgcca ctttattcgc ctctccatcg gaaagagttt tttcgtaaga
    16141 ttgtaaaacc aaacgaaagg ccaacgattt gcgtccttcc tttttaaact catcaaacaa
    16201 aatcggctta tccaacagaa gctcccctgc cgcctcaata attatttcac ccacctgctc
    16261 cgctcccatg tccgccggca cccaaacggc cacatcacga ataattcgcg gataaggcga
    16321 aactgggcga taagaggcat cttgattcaa catttctttt tcaggcaagg cggaaactgg
    16381 accagccatg tccaccattt tctcaaagtt aaattctact aactggtgta atccatcagc
    16441 cgaggcagac tcagtcaccc gcggcagatt accagaccaa ccaatcgcct ccgccacgct
    16501 tgccaccgcc atttctaatt cttttggcgg ctcagttcct tttattttct tgcgataacc
    16561 aaggccaatt gccaacctca tctcttccca accatcctgg ggaaagactg ttccaatctc
    16621 aaaaattttg acaggttcat cgtcaaaaat cacatggatc aaattttctt taagcttggt
    16681 tcctagccaa tctattaagt tcgcgcgcaa aaaagctttg tcggccgcga ggggattttc
    16741 caccttaacc tctccatggt cagtaaaagc ataaccgtaa acttctgtaa aaccggattt
    16801 aaacaacgcc cggcggacca aattggctac cagaaattgt tgttcaaaat gctcggcccg
    16861 ataagctttt gctgacagag gcggcaagat ttcaggttta atcccctcat aaccgaaaat
    16921 ccgcccaatc tcctctacca aattgacttc ttccgttaaa tccagacgcc agaaaggcgg
    16981 cgcaaccaac caaccatcat cttcctcttt gacgtccaaa cccaagcgtg aaaaaatttt
    17041 ttttatttct ccctccggaa ttgtgacccc caagcgctga ctgataaact caggtttgat
    17101 ttttatcgga cttaactggc gttcggcggg ataaacatca accacttcac catattcacc
    17161 gccaaccagt tctttcaaca tcgcagaaaa catcttcatt gcttccgaag ccgtttcgcg
    17221 cgccaattca ttttcaaagc gcttggaggc atcggtctta agttttattt tggaagcgat
    17281 tttacgaatc aaaaccgggt caaagttagc cgcttccaaa atcagatttt tggtctcttt
    17341 tgtcacgccg gccttttggc cgcccttgat accggcaatc gcgagaggac ccacatcgtc
    17401 agcaataatg aaaattgatt catcaagggc cacttcatca ccatctaaag tgattatttt
    17461 ttcaccaggt ttggcaaagc gcgcctttat gccgccttcc actttatcgg catcaaaagc
    17521 atgcaatggt tgtcccaaat caaacataac ataattggcg atgtccacca cgctattgat
    17581 tgaccgttgg ccgattaccg ccagagattt cttcaaccaa tcagccgatt caaaaacggc
    17641 aacattgttc actctcctgc caacgtaccg acgacaagct ttttcgtcct caattctgat
    17701 taacaagggt tctaaatccc ccggcttcac ctcttcccaa atcggtttgg tcaacggcaa
    17761 atcaaaaatc gccgccgctt cacgcgctat ccccaagtga cacaagcagt cgtgggcccg
    17821 atcaggcaag acttttatat caagcgtcca gtcgccttct gctttagcca aaccctccac
    17881 ctcaaaggcg tggaagatta tgccatcttc caatttttcc ggctcgggca aatccggcac
    17941 atgttttttt aaccaattat aagaaaacag catattctag aattgttgca aaaatctaat
    18001 atcgccatta aaaaaaatcc tgatatcttc caccccccat ttgagcatcg ctaaacgttc
    18061 caccccaata ccaaaagcaa agcccgacca ttttttgtca gccagaccaa ctgaacgaaa
    18121 aacgtgcgga tggactaaac cggcgccgcc catttcaatc cagccagtcc ctgaacaaag
    18181 cgagcacgca ttaccgcgaa tcggttcatc tttttcgtcg cgacatttaa tacaaactaa
    18241 atcaaattca aaacccggct ccacaaacgg aaaaaacgac ggccgcaaac gcaccggtgt
    18301 tttttgacta tagagaccgc taaaaaattt ctccaaataa cccttgagat gagcaacact
    18361 cacgttctca tcaaccaaca gcccttcaat ttgatggaat tgcgattcat ggcgcgcgtc
    18421 agtcgcctct tggcgaaaaa ccttacccgg cgccaagatt cgcagtggcg gttcaccacc
    18481 ctgtttcagc atttctgcca aatacctgac ttgaacagcg ctagtctgag ttcgcaaaag
    18541 atgtttgcca tccggcaacc agaaagtatc ttgcatatcc cgcgccggat gaccagccgg
    18601 gacgtttaag gcgtcaaaat tatgatattc atcttctatt tccggcccat ccgcaacagt
    18661 aaaaccaagc tcgcggaaaa tccccaccac ctcattgatt atttgagtca aagggtgaat
    18721 atgcccctga gtgtttaatt tggaatactg aatcagtccc tttttacttg ggcttaccat
    18781 ttaacgatta tagcccggaa atctcctaaa ttcaaatgtt ccctcttcct ccgtttatac
    18841 cttgtccctc atcaaaaacg caggcacgcg acaaacccac ctaagtggca aagttctcaa
    18901 aatcgtgtta aattatgttc acaagccagc atagctcagt tggtagagct accgctttgt
    18961 aagcggtggg tcgggggttc gagtcctcct gctggctcac ttatttgacc tcttcgcctt
    19021 cagccaaaaa tccttctcga tttcctgcca tgacgttttc tttgtgttct ctgatttctt
    19081 ttaaaaaaga tgagacctta ccctgatgga taactttttc tttttctttg attgattctg
    19141 taaagcgata aaatcttttt tccagtctga taattaggta acgaatgacc gagatactat
    19201 ttcgcaaaaa ataaagcaag aaaagataac ccttgctgat tgtttgtttg caaaaataaa
    19261 ccaaacgaaa agccagataa tccaccacca gatgagcaat ggaataatca gccacctgat
    19321 taaaatctag atgattgcgg cgattgcaag agacacgcca agctaagagg ataactagac
    19381 ccgtcaaaga caagagacaa atcgttatta gtattatgtt atccataaat tatggactag
    19441 ctatcacttc cgctttccgc taccgcttca gctgagtcaa aacgcgaaaa acgcgccacg
    19501 tcaatacgct cgccaaattt ctgaatgtac tgtttgacca aggcatccac tgtcaaagat
    19561 ggttctttga caaaggcttg ctccaagagt tcagacacgc tagccggatt catcgcggca
    19621 atatgcatcg ccaaatcatc agccagaact ttaaactcgc tgttattgga cacaaaatcg
    19681 gtttccgcgt ttaattggac catggcgcct atggtccctg tgttatgcga gtagcaagca
    19741 accacgccgg ctcgcagagc gcgcgcggct tttttatcgg ccacttcagt ccctttagcg
    19801 cgcaataatt ctagggcctt ggtcatatcg ccaccagcct cttctaacgc tttcttgcat
    19861 tgagagatag agataccagt tcgttctcgc aactctttga tttgttcagt ggaaatcata
    19921 gctatgatta taacaaaaaa taataaacaa gactaaaaat ataagtcgct ctccaacaag
    19981 gaaaccacta tctatgcttc ttttttgtcg gcaattttat cttcttcttt ttttggctct
    20041 tccggagcgg ccgccaaacc ttctttgtag gcttccacga tttgcttggt gaaaaaggaa
    20101 atgctggtaa tcgccgagtc gttggcaact attggatact caatctgaga aacattgcaa
    20161 tctgaattgc aaagtgaaat taccggcgtt ttgacttttt tggcttcggc cacggaaatt
    20221 tcttcacgat cggcgtctat cacaaaaaca gcgcctggca acctgttcaa gccaacgata
    20281 ccatcaaaat aatgctccag gtcacgcaat tctttggcaa tcatgcctcg ttcttttttg
    20341 gtgtaaacgg acaactcacc tttttcgctt ttcgcgcgca aatcttccat ccgctctacg
    20401 cgctttctga tttctttaaa gttagtcagg gtaccaccaa tccaacgttc ggtcacgtat
    20461 ggcaaaccgg ctgactcagc gatgcttttg ataatcttct tcgcctcatt tttggaacca
    20521 accaaaagca aaattttctt ggttcggcca acctcacgag caaactcttt cgccgcctca
    20581 agcatttcaa tcgttttttc caagtcaatg attgccgttt tgtttttaaa accaaaaata
    20641 aaaggcttgg ttgaagcatg gcggctggat ctggagtagc caaaatgagc cccgattttg
    20701 aagagctcct ggatcaatcc ttctttttgg gcatctttta cttctgtttg cataggactt
    20761 tagcttagca gaaaattttg gtaatggcaa tatctgttta tttttcaggc tcgtctccct
    20821 ctcttgttaa tctttcaccc aaagaaactt tcaggtcgga tagcaaacct tctaagtcgc
    20881 ctgccaaaat cttgtctatt ccatgccagg actttttgat tcggtggtcg gtaattcggt
    20941 cttgaagata attgtaggtc ctgatttttt ccgaccgatc agcggtccca atctgatttt
    21001 tgcgttcaac tgacaacttt ttggcttctt cttcttcgtg gagcgattga aatttagctg
    21061 acaaaatttc taaagctttc tctttattgc gcagttgagt tcgctcattt tggcagcgga
    21121 caacgatgcc ggtcggtttg tgagtcatcc gtaccgccgt ttccaccttg ttaacatttt
    21181 gaccccctgc tccgccggaa cgagtaaact caatttccag ttcattcgga tcaattttaa
    21241 tgtcaatctt ttcacgcacc ggcattatcg ccaccgacac ggttgaggtg tgaacccgac
    21301 cctgcttctc ggttgccgga actcgctgaa cacgatggac ccccatctcg ctttttagtt
    21361 catcataggc cccggcgccg gcgatttcaa agactgcttc tttgtaaccg ccagactccg
    21421 acaacgaatc gtctattttg gaaaaagacc aatttcggct tttgacatag ctcccataca
    21481 tttcggctag atccaacgcg aataaggccg cttcatcacc gccagccccg gcgcgaattt
    21541 ccataatcaa gctttttggt cccggcccca tttcaccttt gggcgataaa atctcagcca
    21601 tcttgtcaat caattcttgc cgctgttttt ccaatttttt tatttcttcg cgtgccaaaa
    21661 cagccatctc tgaatcagtg gctaaggttt tgttttcttc aatttgtttt gtcagctcat
    21721 cacaaagact cgccaaataa gtggttttgg aattattgcg gtaaatttgg atttcatcat
    21781 ccatagtaaa agaagtcggc caaaatagat gacaaaaagt ctaggcctcc ttgtccgctt
    21841 tggcaatttt tttagctttg gctgctttag atttagccat tcggcccttg aatttttcca
    21901 ccattccagc cgtgtccatg actttttcgt tgcccgtgta aaaagggtga caagcgctac
    21961 aaatttccac tcgcaactta tctacggtag aaccgatcgt aaaagtgttc ccgcaggcgc
    22021 aggttacttc cgccttagca aaatattttg ggtgaatatc ttttttcata gtatctggta
    22081 ttttgtatta tagcgtattg cataaactat ggttttctcc agatcttggg tttgtcttcc
    22141 cattccagat tctacagcct agatacaata ttctatattt cacacactat acaccatttt
    22201 tagtcatcct gcaagatttt aatttgttgc tcgtaagtgg ccactaattt catcacctgg
    22261 tcaccataaa aagcgttttt cgggttggcc caattgccac cggcgtaata tttgagcgcc
    22321 gctgttcgtt cagccgtaaa agtgccagcg ctcgccccca aatcgctcaa ataagtggcc
    22381 gaggccgcaa acgcgtcttt ggggttccaa ggatttggcg ggttgttgcc cgtcgcctta
    22441 gcaatccgcg attcataagc agcccaagtg gaaggaataa actgcgccgg ccccatcgcg
    22501 ccaccccaac cgcccatata aggacaagaa atcggttgtg tgtcagtatc caaaccaaga
    22561 ctcttggtaa tccgcaaaaa cggcgcatgg tctcgttccg gcttcatcac cactcgccaa
    22621 cttttttctg gcggatcacc cggtcgatta cacgtaccaa cattttgacc taagttagac
    22681 tcctgagtga taattgctaa aatgaaagcc ggtcgaacgc cagtcgccgc cgaaacttgt
    22741 ttggccaact ccaaagcctc accaaaggta atcgcttgga cgttacgcaa acggaaaagc
    22801 gcgctcctaa tcgccgctcg ctgtttttct ttttcagcaa taattgtcct ataagcgccc
    22861 tcttgggttt tgctcatatt gagataaacc tgcttttcgg cattaagcct ttcaatgttg
    22921 gccttttcaa cctcaattgc tttcttagcg ttggtttcag ctgctcgcct cacttctagt
    22981 tgtttttttt cttgctcggt caaatttttg gttgacctcg tgctagaaag agttttgtgc
    23041 attgattgct gaataaagta ataacggtca gcttccacaa acaaatcaga aacattattt
    23101 ttatccatca ctaattcaat aaaggagttc tcgtcaatct cgcgggttgc tcgcaaaagt
    23161 tccgccaacg acactttgtt ttttccgact ttttcgtcta attctccaat atattctact
    23221 ttatcgctga tgtcaccgcc taaacgttca atttccagct ttttcttttt gatattcagt
    23281 tgcgcggtat tgattttata attcaaaata tcaatatctc gctgaataga ggccgtttct
    23341 ttttgcttag tgcttaacaa gcctttttgc tcgctaatct ctttctcaac cttggccaat
    23401 tcagctttca gtttggcctc tttggcggcg atctccgctt cagtctcggc ctgagttgga
    23461 taaaacgcac tctgcgaagc aacaagcgcc aaaagacaaa taaaaattaa agaaaccgcc
    23521 ctcggacatc gtcctagatt ttcatttgat gacatatgaa aattataaca gaaaacgctc
    23581 agtttaaaaa ctgagcgttt catttaagtt ttattctgag attttattca gatttttctt
    23641 cagcggaggc tttctcactg ccctcttctt cttgtttgcc cttcttctcc acttcaattt
    23701 gagacagatc aatcggcgct tcttcaacca ctacttcttc acccgccttg ctgatggaaa
    23761 caacgacctc ttccagctcg gccaagactg ttaccccaga aggcaactta atgtctttaa
    23821 ccaaaatctg gctttcaaat gtcgccaaac cggaaacatc aaccttaatg tcgtgcggca
    23881 agtcttttgg caaaacctca atcggtaatt catgcataac tttcaccagg ttaccaccaa
    23941 gttctttgac ggccggggct acgccctcga agactagcgg cacatcaacc tcaatttttt
    24001 tatctttttg gacaacataa aaatcaacgt gaatcggttg gcccttaatt gggtgatact
    24061 ggacctcgtg aatcaacaca tcatggccct tgccctccat gtccaagctc acaatggaag
    24121 attcgccggc ttcttttaaa actttaacaa actctttggt atcaaccacc aacggggtgg
    24181 cttcttgttt ggcgccataa acaaccaccg gcacctgccc tgcttctctg actttcttta
    24241 attgtttgcc aaaaacatca cgtttctggg cttttaactt aattgtcata cttgaggcat
    24301 tatatctaaa aacagaagaa agtaaaggcc caaaagcgaa acgagcccaa attcaaaaaa
    24361 gaggccgacg tttccatccg cctcactaaa cggttagaca tttgtggttt tcatcatttg
    24421 ttttatccgt tccaaggtat ggtccaagac agcgaccgaa aattcggacc attcaaaaaa
    24481 tcggacaaga tggttagggt ctccctcctc gtgatccaga ctgaaaatat attctcccgc
    24541 cttgtttgtc accaaatacc tgtcaccagc cggaaacacc accagacacc cagatttgcc
    24601 attccctggc tgcaaaaact tggaactggt gctgacaata aaaacattcg ccgtgtcagg
    24661 gtcgagaacc agttctattc ttgtttttgt ggaaatctca aacctgacta tcgcgcaaca
    24721 aataatctcc aaactgcggc gatccaaaca atcaaggcaa accttcatcg atctcccctc
    24781 cccctaagtg aaaaatcatc cctgtctcca aagttccact ccagcgtcat tttattgctc
    24841 gaatttaatg atttgatgaa gaaagtttta gcgtcggcta aaaagattca atcttttttc
    24901 cacaacttgt ttgacagcga tgattggccc tttgagatat ttatagggag caatttcctc
    24961 cgggttttct tgtaacgatt caattagggc ctgcttatag gcaactcgca attcggtatt
    25021 gatatgaaca atcgccaccc ccgcctcaat cgccgccaca aagtcttcat cggtcaaacc
    25081 agacccccca tgaagaacca agggcactcc cgtcacgtcg cgtatttctt tggttcgctg
    25141 aataaacagt cttgggttag cggcatgttt caacatgcca tgaatgttgc cgacagctgg
    25201 agaaaaaaga tcaacgccgg tttctttgac aaacctctca gcctcttcaa cttttgtgat
    25261 agacgattcg tcaagcgcga cgccgactgg caactcatcc agcatttttg acgacttacc
    25321 aatatagcca agctctccct ccaccaatat ttcaggattg accgaccgtg ccaactcaac
    25381 gcatttttta gtttcggcaa tgttgtcttc tggtgagagt tcgctccggt caaaaacaat
    25441 caagtcaagg ccggcgccaa tcgcctcttg cgcgcgcttc aacgaagaag tgtggtcggc
    25501 tgacaaaaaa atcgactgat taaattcagt gcgcgcgcta tccaccaagg cccgcgcctg
    25561 acgcaaacca ataaaatccc tttctccttc tgataaacca ataataactg gcaagtctaa
    25621 attttgcgcc gcccgaacta ccccccagaa tccttccaaa ttagagatgt tgaagtggcc
    25681 aagagctgtt tttgtttcat tggcctcgct gattatttgc ctaagagttt tcatgctata
    25741 attataccaa tcaaatgaat aaattaaaat atggacaagt ttaatcttct aaccattggc
    25801 gacacggcga ttgatgcttt tgtcagattg aacgaagctg aattatactg cgatattaat
    25861 cgcgaggagt gcaaaatttg tctgaaattt gcctccaagg ttccttatga atcaatctcc
    25921 gaaattcccg ccggcggcaa cagcgccaat gttgcgatta gcggcgccaa attggatttg
    25981 accaccgcct atttcaccaa catcggcgat gatgacaatg gccaaaaaat tttagccaaa
    26041 ttgaaaaacg ccaatgttga tacaagtttt attaaaattc acgccaactt agccaccaac
    26101 tataactttg ttctgtggta tgctgatgac cgaaccatcc ttgttcgtca cgaaaattac
    26161 ccttacgaat tacctaccct cccccaaagc gactgggtct atctaacttc attaggcgaa
    26221 aaatcactaa ctctgcaccg tgagttgatt gatcatttgg acgataatcc ggaaaccaaa
    26281 ttggccttta gtccgggcac aatgcaaatt cgcgccggac gcgaaacgct agctgaaatt
    26341 taccaaagaa cagaaatctt ttttgccaac aaacaagagg taatggaaat tttaaacacc
    26401 aaggaagagg aggcaagcca actggccaaa gaaataagga atttaggtcc caaaatcgta
    26461 gtcattactg acggcaacca aggcgctttc gcgttgaccg attccggttt ttggcatagc
    26521 ccgattttcc cgactgaaaa taagcttctg gagcgcaccg gcgctggcga tgcttttgcc
    26581 tcagcctttt tggccgcctt caataaaggt ctgacggtac cggaagcact ggcctggggt
    26641 gccgttaatt cggcctccgt cgtccaaaaa atcggcccgc atgaaggatt attgtcagcc
    26701 caagaaatag aaaaacggtt ggcggccagc gtttttgaag taaaaaagat tgaagaaata
    26761 aaattctaaa aaactttcca caataaaaac cgccggcgtt gccggtgtgg ttttcattat
    26821 ttcttaaaat acaatgtttt ctccactcag attatgcttc tttttatact catcaaataa
    26881 gccccaatat gagcgagctg agcgctcagc ctccgtcaaa ccaatttttt gaattgttgt
    26941 ttctatctct tctggttcac cttccacttc cacaaaattg ccaaaaggta accgatcaag
    27001 cgaaatcact gtctcgtcca aatgataatc agtccgatat ttttccataa taaattcacg
    27061 cgtaaagccg agaatgttca atatttgtcg aactttttct gtatcaccaa cttctgtttc
    27121 cacctcttct ctttctttaa gacctctcgc ctccaattca ctaccatcaa ttcttctttt
    27181 tagagtcaca acatttttgt cgccagtccg cactcgcaaa aatgttttat ttgcctctaa
    27241 actttgttcg ggtgtatcaa aacgcactgt tcgctgaaaa aactccggct ggagtaaaac
    27301 accaccagcc acttttattt tttcggccaa accggaaaaa tcagccacca acaactttac
    27361 ttctatttct ttgttattat ccttgttcat tctttctttt gattactctt ctgaccgctt
    27421 caataatatc gtctatcgcc aagccataat gttccaaaag ttcggccgag ctacccgact
    27481 ggccaaaccg gtcatcaaca ccgacaaatt ccattgggag agggtaattc tcgctaagaa
    27541 gttcggcgac cgcccctccc aagccgccag ttttttgatg ttcttcggcc accaccaccg
    27601 cgccggcttt tttcgccatc gctaaaatag tttctttatc caaaggtttg attgtatggt
    27661 tatttaatac ctcgcactca attccttctt ttgctaattt ttgagcggca ataagcgcgc
    27721 gataaaccaa aagaccacag gccacaattg ccacttttgg gttttccgac tcccgcacca
    27781 ccaaggcttt gccaatttca aatggcgctt cagggctggt aatgattgga gcttcaacgc
    27841 gcgataaacg caagtaaacc ggaccccggt attcggccgt cgccatggtt gcttttttcg
    27901 cctcttccgc gtccgccggc actaccaccg tcattttggg caaaacacgc atcaaagcga
    27961 tatcttcaag agcttggtgc gaggcgccat ccggcccgac cgacaaaccg gcgtgcgaac
    28021 cgacaatttt gacacccacc tcgttcagac aaatggccgt cctgattatt tcccaattgc
    28081 gtcccggcga aaaaacggca aacgaagtca taaatgggac tttgcccatt accgccaaac
    28141 cggctgcgac tgtcgctaaa ttttgttcgg ccactccaac ttggaaaaat cggcttggaa
    28201 actttcgccg aaaaccgtcg agccgcgtgg aatcagaaag atcagctgaa agcgctacta
    28261 cttgctcatc tttttccgcc gccgccatta tcccttcgcc aaaaccatcg cgaatcgctt
    28321 tttgcgcgac actcaaatca aagactttgt ctgctaaaat tttcattcac tttaattata
    28381 acaaaagcca acgacgaaga acaacaggca accaatttta ataatctttt cactgaaata
    28441 tgacaaaaat ttaaatatgc ctattacctc cattctgact ggagaaattt ttaagaggaa
    28501 agccacggtc aatcaaagcc gagctttttc tctccgaaaa agctcggctt tgattttgct
    28561 cggcatcctg atcacttatg gcatatttat tcttaaggtg caaatttttc actcaccacc
    28621 ggtattattc ttgctccttt attccgccgc aatgaccgct ttccaatttg gccgcctact
    28681 gacagtcctg ttttacgaag aatctctaga aagaatccgc ggtaaatata gcgccaactc
    28741 tctgatcggc caggacccat atgaaccgtc tgtcagtttc gttatcccct gcatgaacga
    28801 agaaaaagca attcgccata caataaacaa gtgttttgag gttgattatc caacccataa
    28861 ggttgaagtt ttggtgattg acgacggtag caccgataaa acaccagaaa tcttagatca
    28921 gctaaaccaa gagaacgatc gtctgacagt gataaaattc gctcaaaacc gcggcaaaag
    28981 atacgccatg gtggaaggtt tcaagcgcgc tcgtggcgaa ataattgttc aattggatag
    29041 tgacagccac cttgcccaag aaggtttgcg gcccttaata gaaccgtttc agaatccctc
    29101 tgtcggcgct gcctgcgccc actccgatcc ggccaatgtt gataagaact ttctgaccaa
    29161 aatgcaagcc gcttattatt atatgtcttt tcgtatttta aaggcggtgg aatcaacctt
    29221 ttatactgtc ttttgctgca gcggctgcgc ttctgcatat cgtcaatcgg ccgtcttagc
    29281 tgtcttggac gaatggtcac gtgaaaattt tttaggtttg ccggtgatgt atggcgacga
    29341 ccgctctctc acgacccgtg tcatcaaaga gggctacaaa acaatctaca ccgccgaggc
    29401 tcaagcttac accatcgtgc ccgaaacttt ccgacaacta attattcagc aagttcgctg
    29461 gaaaaaatct tggattataa acgcaatcaa aaccggtaaa tttattatta cccaaaaacc
    29521 cttcattgcc attctttatt tttatccgct ggttattctt tctctgataa acccgtttgt
    29581 cgctctttac gctctagttt atgtgccgat tgtttatggc atttggccaa ttttttatct
    29641 tgccggcatt tttttgatta ccgccctcat tgccgtctat tacaaagttt acggaccaca
    29701 aaaccgttac tggccgtacc tctttgcctg gggcgcgttt agtgttaccg tcctggcccc
    29761 ccttctcttt tacgccgccg cccgcatcca aaaccggagt tggggtacgc gctaatattt
    29821 tattaaccaa actttaattt tcataaagaa caagcctcat cgtcagcatt gaagactaaa
    29881 tcagctggcc attgacaaac tacctggtta aaaaaagtac gtggcaacgg acatcggtaa
    29941 ttaccccaga tgtaagctta tttgccaaag aactagtccg gaatcgctgt gccgaattga
    30001 cttactgaaa aacagaacca gaaaacacaa aagaatcttg agtagaatta aatggagagg
    30061 tgtgaatttt agaagtaaaa caaataaaat aacagccgaa acgtttgata agaaaaaaac
    30121 aaagaaccta gaacatggtt tagcgttaaa aaagccccaa tttctcaatt ggggccatgg
    30181 aaccatgaat agactacgcc cggcgaccca gaggcatgcc gccacccatc actagccaac
    30241 tggccagatg cccagtaacc gtatcggcgt acttgcgctg atggggccac atggcttgat
    30301 ctggcattgc caggagcgac agagcttgga tggcgcagat gaccaacaga gaacaaaggc
    30361 gccaattgct ttcgcctttt ctgtcacact ccacccagtc cgggtgcatg gtgttgaaca
    30421 cgatgacgcc ctcatccaag tccagttccc agagcttggg cgagtctggc atctcgtcat
    30481 aggcgaattg caacccatga ctaccccctt gaaccaaccg acgttttgag ccgtttggac
    30541 cacgagaggt gagatgcggt tcgtcatgct cacggattgc ctcttcttgt gagcgtttag
    30601 atccctcacc accaactttc ttttggcttc cggtgatggc cttcgacttt tcttcctgtt
    30661 cccgagtggt ttccctctcg gaatggccaa gaccgatact gccatagcga aaccttgaca
    30721 gcgccgcctt caagtcttcg ccacggggag actcgcgaaa cagacggtca agtcttgaaa
    30781 gagcgtcgtg cccaatttct tggtaccgtt cactctggcg aactcgacgc tgttcgtcca
    30841 agtagtgctc accctcttcc ctaaaccatg tttcaagaga aacacaaagg tctatcatgg
    30901 cgtcgtttct ctcaaaacaa gttcgactgg gagtcaattg caccagctct cccaacactt
    30961 ctccttcaaa tatcccactg cctagagcct ccataacccg cgagtccaga agctccttgg
    31021 ccgaagatgt ccgaaagtga ctgaaaggaa agcggaaatc attcccggct tccccaaact
    31081 taatgttcac ccgaccacgt gaatcgtggc gctttgctag gtacatccga acaatcacct
    31141 caccggaaga ctcccggtaa taacgccgtt cgggcaacct tgtgccggtg aacttggctg
    31201 ctgccacttc cgccgacgac tcgttgccgg catagtcgac gaagtcaact cgaagccgag
    31261 tgccgagcct ttccatcttg ctggaatact tcatcgtcgc actctccaag atacctttca
    31321 tggtcatctc ggataaggcg cgatctttga cgtattcctt tatctcgatg gcagtgcgcc
    31381 accacacatt ttggccagaa cgaacgttta cctgatccgg cgcgaaacga agatcttttc
    31441 gctcccgcca atcaacagac acgccagaag cttccgaatc aaccggaaag gtccaggtga
    31501 cgtaggcctc ttttcttgga gccggacaag aagttaccga gaactcttga cacttgcccc
    31561 aagctgaaat ggccccgata ccaaactgtc cgaggtcttc ttccgattta agagtgtgcc
    31621 caatggattg gctcaccctt ttctcgaaag cctgctggct catcccgcga ccgttgtcgc
    31681 ggatagcaat gaatctcgtc ttctgattca ctctgagctg aaccagggtt gcccccgcgt
    31741 ccaacgcatt ctgaatcatc tcgtcaacca ccgacgccat gtcggggtag aaacgagcaa
    31801 gattcttcaa agtgccgccc atgtgtacag acaaagaagt cttgcccgag ttcactgaca
    31861 ctcccttttg tccacttttc atctgacttt cctttcttgt tcttttgcta ttgctagagg
    31921 acccggtgct cacgctcaag tgttctttcc cgagcccgtt ctcccgccag tttggtcaag
    31981 gccacggacg tttgatggac agtttcccgc ccagccaatt ctcgcaactt ctcccggtca
    32041 ttggcactaa aaacatccga gcctaccagt tgtgccaaag gaagacacgc ctgaagaaga
    32101 cgcgccaaac cttcaatcac gtcgcgacgt ttttggttat ccaaacctcc aaccaatgtc
    32161 gatgattttc cctcttgatc agacgaccaa gaattgccct cctctctcga tggaagttgg
    32221 agtttttcgc gccaagtcgc gaggtttctt tcgagctctg tctcacagga tttacaaaac
    32281 tttctcatgg tttcctgtct gcccctcaaa ataccacttt cacctcgcaa aaccacgaga
    32341 agatgtgatg gtttcttgta gttcaacctt tggaccgcct cttcaaaaga gatcgcccca
    32401 aaggctatgg ccttgccgaa gtcatagata gggcctttca gaccatccaa ttcttcagca
    32461 tggtaaccca gaaggttcaa aaagtggacg accttgagag ctttctctcc catcggacga
    32521 ccacgagaaa catccctgag ccagccattg actgtcaaag aagaaacccc gacgaactcg
    32581 gccagaagtt taatgtcctt agccgaagcc cggacaccaa attccttcag acattccatt
    32641 aggttcatgt tcagctcctt cttttgctta ttgctagaga accacaggac gagccaactt
    32701 ggctcgccag cctcgcgcaa actttcgaac atcacccaaa agcacgacct gcccaacctc
    32761 gggcggacaa tcatgttgcg accaactttc caagctgaaa gtgatgcttt cttccaaagg
    32821 aagagggtag ctaccacctt cgcctgttcc gcactggtga caaacagcga aagatccttt
    32881 ttcagaagag caaatcttgc cgactattgc ttctatctga ccagtcattc caaacctcct
    32941 ttctgatgct gttgcttgtt ctattccttg gttatcaagg aactaattcg gtcattataa
    33001 taatgtaaaa actcgtatat gtcaagatat gtcggcaatt ttccccaaca atttgtttta
    33061 aaaaatattt aaactgatgg ctgaaaaagt taccccttct ttattcaatt tcttttaacg
    33121 ctcgttcagc ttctttggcg tttggggttt tgccgtgcca ttcatatttc ctctcccacc
    33181 ccgacacgcc cttgccaggg attgtatgag caatgataac cgacggtcgg ccaaaaaccg
    33241 ccttggcctg accaaccgcg ttaaaaattt ccgggaaaga gtggccatca atctcctgaa
    33301 cttgccaacc gaacgcgcgc catttgtccg ccaaaggctc gagcggcatc acttcttcag
    33361 tcacgccgtc aatttggatg tggttacggt caacaatcgc aatcaaatta tgaggcgctt
    33421 ccttgccggc aagcataacc gcctcccacg tagaaccttc ttgtaactct ccatcagata
    33481 acaaacaata aaaaaatctc tgcgaccctc gccccttatc taaacggtca gccaaaacca
    33541 tccccagcgc ctgccccaaa ccttcaccaa gtgggcccga gctcgtttcc aaacctggta
    33601 actttaaccg ttcaggatgc ccctggagac gggacccata gcgacgcaag gattttagtt
    33661 cttctgaagg aaaataaccg gcgcgcgcca tcgccgcata aagcgccggg caagcatgac
    33721 cgttggagac aattaaacga tcgcgttccg gccaatccgg tttgtcaggc ttatggttca
    33781 aaagaccaaa ataaagcgcc gcgaaaatct ccgccaaacc aagcggccca ccggtgtggc
    33841 ctgaaccgga agtgaccaac atctcaacca ccgagcgcct aaccgctcgc gcctggtccg
    33901 ataaatattc aagtttttgg tcagtcagta acataaattt aaatcaaagg ttaaaagaca
    33961 aaagttttta tatcatatcc cataagttat ttcttttctt aggcttatcc tcttttcact
    34021 tttaacttaa tgttcttagc gcttcttcaa tgtttttgct ccgccaaata tatgaaccaa
    34081 ctaccaaggc atcaaccccc gcttcagaca attctgccac catctccggc ttaatgcctc
    34141 catcaacaat tattttagca tcagatgcca attcccgcag ctcttttatt ttaggcaaaa
    34201 cccgagcgtc aaacttttca ccctgataac caatattggc aatactcatc aactgaaaaa
    34261 tcttaacttt gtttagatat ggtttgactt tttccaaaga tgtttctaac tccaaagcca
    34321 ggccaatttc gcacgggtta agtccgttgt caaaacggct caatagagaa tccagctggt
    34381 cggttgattc aacgtggata attattctgt ccaccaaggt ttgccaatct tccaccaccg
    34441 tctccgggtt tcccatcatt aagtgtgctg ataattttat ttgcccgtca atttcgcgca
    34501 aatcttcttc agcttgccag ctaacattcg gcacaaaaac accgtccatc acgtcaagtt
    34561 caacccattc tactaagcct tccaacttgg ctaatttttc tttgacctcg gcaaaatctg
    34621 aagcaatgat tgagggaata atattcatag gcgatgccaa tttgcaaatt caggcaaata
    34681 atactgatag gataactcaa tttcttttaa attagtttca tttatagaca ttcgcatgtt
    34741 taagccacgc ttaatatttt aacgccccac cgacattgtc catatcatca attccattta
    34801 ttcgtctgag gtgccgttct tcgcctgaaa agtctgtgct caaccacaaa tcaaccgctt
    34861 ggattgcctc gccgggtgaa acaaaacgag cgccaagcga caaaatattg gcatcattgt
    34921 gagcgcgaga cagttccacg atatccgggc tgcccccata ataaacggcc gcgcgcacgc
    34981 cagcaaaacg attggcgcaa atggcttccc cttgaccgga cccacccaaa ataattcctc
    35041 ggctacccga tttgccctgc actaacttgg cgacaatttt gacgtaatcg gtgtaatcat
    35101 cttcgggctc taaagcataa gcaccctggt cttccactac caacccacgt tccttcagat
    35161 gattttttat ctgctctttt aaggcaaaac cggcgtgatc cgccgcgaga tatattttca
    35221 taaattaatg ataacctata ccaaagtcaa aagtcaaaag ttaaaaagtc caccgccaaa
    35281 aaccatttag tagatgtatt atcttttatc caacttatct actttttact taaatttctg
    35341 acttttaaca tttatcctga ttctctatct tccggctttc aacacatgtt ccaccagcgt
    35401 atgggcatat cccatttcat tgtcatacca agccatcact ttgaccaagt ttccaccaac
    35461 caccttcgtc caactcaagt cggcaatgga agcatactta ctacccaaaa tatcagagga
    35521 cacaatcggt tctttggtca ccgtaaaagt tttttgccaa cgcgggttgt tggccgcttt
    35581 ttcaaacgcc gcgtttactt ccgccacggt tgtgttcttt ttagcaacaa atgttacatc
    35641 aatcattgaa ccagtaataa ctggcactcg cactgccacc ccatcaaact tgccggccat
    35701 ttcaggaatt acttggccaa ccgcaatcgc tgcgccggtt gtagacggga ttaggttaat
    35761 cgcgcccgcc cgaccacgcc gccagtcttt agcatcggcc acatctacca atttttgagt
    35821 cgcggtatat ccatgggttg tgttaagcag ggctttttct accccaatcg cctcatcaag
    35881 aattttaatc accggtgccg cgcaattagt tgtgcaggag ccattagagg taattacctg
    35941 gtcggcaatt ttgtcatcgt tgataccaat cagaaaagtc cctccgggaa taccaggtgc
    36001 cggttcatct ttaacgggcg ccgaaatcac caccttgcgt gcgccggcgt ccaaatgtgc
    36061 ctttgatttg ccataggcct caaacacgcc ggttgattcc aaaacgacat ccacccctaa
    36121 ttcagcccag ggcaagttag ttggttcttt ttcttgaaag aaacgcgccg atttgccggc
    36181 agtcatcagt ttgccgtctt caaacttgat ttcaccatca aactgaccat aagcgctatc
    36241 atactttaaa agataggcca gattggccgg atcgcccaag tcgttaaccg cgaccaaatt
    36301 tatttccgga ttcttgaccg ctagcttaaa aaacgcccgg ccaatgcggc ccaagccatt
    36361 gattgccact tttaccataa ttttaaaaat tattttttgt taatagttta attttaacaa
    36421 aaaaaatcaa gaaaaacatc caccttgaaa aaatatccga ccaacgcttc aggccaaagt
    36481 ttattttact gattctaacg attaacaaca gccgaggtgg aggttgagtt tttgtttatt
    36541 tctttggctc taggcttgct tgttataaag gcaatgttta aagcaaaaaa cagcaagtac
    36601 aaaaacatag acactccagc caaattttcc cctattgatt cagggatgaa gtttacggga
    36661 ccacggaaaa gatgaccaaa aagcctctcg attccctggg catcaccaaa atcagcccca
    36721 tcaataataa gcaaacaagc cgccgcgtag acacccccaa gcaataatag ccacttacgt
    36781 cctgttttct ggtctccttt atgatattta atcagggtaa taatcgtcaa aacagtcaaa
    36841 attggaagaa taaaaacaag aaaaaagaca aagccccaac cgccccaggt cacaaagggg
    36901 aaaccgacaa taaataaaaa gtaagccaac cccagccaag aaaatttttt atccataata
    36961 acaatagaat ttagctgtta atctttcttt tgtttttctc agatttgaat taggattaat
    37021 tttatcagca atctctcgcc ttcgttctat gttatctatc ctttcttttg ttcaaacaat
    37081 tttttgagta agcctgggtt agccgaaccc ttgctttcct tcattgcttg gccaaccaga
    37141 aactgcaacg ctttttcttt gccggccttg tattcttcaa aaacattttt gttatcaaca
    37201 atcactttat ccacaatcac gctcatcgca tcttcgtcat tcacctgaaa cagacctcgt
    37261 tctttggcga tttggcgagg ctcgccgcct tctttaatta ttatcgccaa aatatctttg
    37321 gcgcctcgtg atgacacttc accccccata atcattttga gcagttccgc gtaatctccc
    37381 acaggcggca aacgcaattc accggccaat gcggccaagc ccaaaatatc agaggtaata
    37441 tagttggcag ccaccttgag atattctttg gtttcgtcca aagcatgatc caaaccttca
    37501 gtcagctcgt caaaaaagcc ggccaaactg cggtcttgca caaaaacctc tacatcttca
    37561 gttttcagac caaaaagttc cgcataacgg cgacggcgtt gccaaggcaa ttcgggcaaa
    37621 gattcaacaa cattacggaa ctccggaatc tcgctgataa ataatttcgg caaatccggg
    37681 tcgggaaaat agcgatagtc atgcgaggac tccttcaagc gctgggaaaa agtggtttgt
    37741 ttgttttcgt cccaaccacg ggtttcctga attacccgtc ctccagcttc caacacgcca
    37801 gtctgacgct taatttcttc atctatcgcc ttctctaccg tccgaaaaga attgagattt
    37861 ttaatctcca ctttggtccc caacttatcg ccttccggcg cgaccgaaac gttggcttca
    37921 atccgcatct gacccttttc catattggcc tccgaagcgc ccaaataacg caataaaagc
    37981 tgaagttctt tggcaaaagc cgaggcagtt ggaccgtcat taatcaccgg ctcggtcacc
    38041 aactccatta gaggcaagct ggaacgattg aagtccacca aactctcgcc agttttgaca
    38101 tcgtgaaaag atttggcagt atcttcttcc aagtgaactc gcgtgatagc gacgcctctc
    38161 aaactgcccc ctgacactaa tggatatttg tcctggctaa tctggtagcc tttggggata
    38221 tcaggataaa aatagttctt acggtcaaac tcggtaaagt cggccaaacg tccatccaag
    38281 gccacgccga ctcgcaaaat atgccaaatc gcgtccttat taatgaccgg caaagcaccc
    38341 ggttgggcgg tacaaaccgg acaaatatta acgttcggct gtcgctcatc cgggttgtta
    38401 aggcaccgac aaaacatctt ggtcttggtg tttaattcga catgaacctc caagccaatg
    38461 gttggtttat attttatttc actcatagat tagtcaatta tacgcaaaaa cggcaaaaac
    38521 tcaaaatcgg caagctaagg caagcaaaaa ccggcccaac gacgtcaggc cggtttttta
    38581 ttcaactttt ttattgccca atttttcgtg ggaaaaagcg attctttgcc cacctgccta
    38641 ctttccaaat tatcaacaaa acaatagcaa tcgttatcaa ccatagaaca atcaatggca
    38701 acaaagcaat aaacgcaatc ataagattga catattttac cgaacctttc accaaagtgc
    38761 gcaaagattg ttttatttgc gttaatggcc gccaacgaac gccaaacact tcaatgtccg
    38821 cctccgacgt caatgaaacg gagattgtgg acatctcaac ctgtctgtcc atgtatttta
    38881 tttgtccctc aattctttct atattgtttc gaatttgcga aagctctcgt tgaacattaa
    38941 ggatttcatt taccgtttgc gctttcttta aaacttcaag gtatttatct tcagtagcat
    39001 tgtagttcct taaccttgtt tccaaatcaa cgtattggtc ggttacgtct tgggtgctga
    39061 cctcttctct ctccacttta accgttgcat cttttatttt atcaatcgtc tcatcaaatt
    39121 tgtcagatgg gaccttgatg gtgatatggc cagattttat gtcgctggca atttcataga
    39181 tcctagccac cgaaacaaat ccacccgctt gttccgcgat attggttata gtttcaaccg
    39241 cctcctctgc cttttccaca attaccgaca ttgaaccgtt gcgagaaact ttcctctgag
    39301 gcaagacttc tgtcgtggcc gattttgtgt ctgcctcttg gtagtaatca gctgaatcag
    39361 gcgataagac agcctcacta ttctgaaaag aagttaccgc cactctagca ccggacagtg
    39421 aggttatgac cactaaacaa ccgacgacgg ccaccagaac caaggctcct aaaacagcca
    39481 ggattgaaaa gattattttt ttcataaatc accaattaaa ttaataaaaa tcaactttgt
    39541 ttattataaa acaaaaaaga tttcataaaa agccttagct caaaagccgg acttcttctt
    39601 ctaaagctat gccaaatttt tgtttgacca agcgcttgat tttttcggcc aattttttta
    39661 agtcacgcgt cgcgccgccg ccgcgattaa tcaaaaagtt agcatgaaaa tcagccaccg
    39721 ccaccttgcc agccgaaagc cctttgccgc cggaagcttc tatcaaatac ccgactgaaa
    39781 cttttttatt aaaaatttta gttgggtcaa tctttttcag gctagttttg gagatacgcg
    39841 acaggggcac atttttaaaa aaactgccgg cgcacaagag gccattatat ttattgtcac
    39901 gaacgataat taaattgcgc gctactttat gtagggccag tttgtcgccc ttttttaatt
    39961 caaggcaaac ccgtaaaact aaccaatttc tttttttgaa aacactgtcg cggtagccaa
    40021 aacggcaatc agattttccg agtcggcg
//
LOCUS       DAOOLO010000002        30311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_1315_length_30311_cov_6.972402, whole genome shotgun sequence.
ACCESSION   DAOOLO010000002 DAOOLO010000000
VERSION     DAOOLO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 30311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30311
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_30311_cov_6.972402"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLH96_00190"
     CDS             <1..224
                     /locus_tag="PLH96_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60230.1"
                     /translation="FPGIVLIGKSYEETLDRNRKGPRWGTTQRQQEMEAKNFFFEEGP
                     LFKKEAIKYGYPVFETADEAFNYCLEKLS"
     gene            complement(634..705)
                     /locus_tag="PLH96_00195"
     tRNA            complement(634..705)
                     /locus_tag="PLH96_00195"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(670..672),aa:Glu,seq:ctc)
     gene            complement(854..2161)
                     /gene="murF"
                     /locus_tag="PLH96_00200"
     CDS             complement(854..2161)
                     /gene="murF"
                     /locus_tag="PLH96_00200"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPB60231.1"
                     /translation="MKNTLKMLKKGFRPVVVVILRWEAKLVLKKYKPKIIGVTGSVGK
                     TSTKDAIYEVTKNYLSVSKSEKSYNSADIGVALTILGCQSAWYSILGWLDIFFHGLFL
                     IIFKKDYPKWLVLEIGVEYPGEIAQIVKWVKFDHAVVTFLPDMPVHVEFFSSPAEVIK
                     EKMLLAKAVPAQGKVLINADDKNVAAHLGGIKGEILTFGFNDLADYRAEAQGVLYDET
                     GLPSGLTFKFEHNGKSLPVRVGGVVGEHQIYPVLAALALGDILGVNVIEMIEVLKDYK
                     PAAGRLRVLPGLKNSVVLDDTYNSSPAAVAAGLKTLSLLQVKGRRIAVLGDMLQLGTY
                     APEAHREIGYLAAEVCDLVYTVGLRSKFIDEVFIEKGLGEKVRHFDDSLSAGQTLQNE
                     IEAGDVILVKGSQSMRMEKTVVEIMAEPDKKEKLLVRQEAEWL"
     gene            complement(2191..>2985)
                     /locus_tag="PLH96_00205"
     CDS             complement(2191..>2985)
                     /locus_tag="PLH96_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB60232.1"
                     /translation="LDAGAVTTSTTFNDTGCITVSGKKICNYDGRARGVVPMQEVLNQ
                     SLNVGMAFVARQLGQEKFRAYYEAFGFGEKTGIDLPGELNNRIQTIYEPYEINYVTAA
                     YGQGLSFTPIATARALAALGNGGKLIEPHIVKEINYQNKLSRKIEPKIIRQVIKKETS
                     EEISRMLVEVVDEKLAGGKAKLEHYSVAAKTGTAQMVNPETRKYYPDRYLHTFFGYFP
                     AFNPRFIIFMYIKDPKGVRYSSETLTEPFSNLTKFLLNYYQVPPDR"
     assembly_gap    2986..3085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3086..3673)
                     /locus_tag="PLH96_00210"
     CDS             complement(<3086..3673)
                     /locus_tag="PLH96_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60233.1"
                     /translation="MKYNSGLRVKFLAFGIFALAALFIVRLFFIQVVKADHYRAEADK
                     QHFSPGSARIYDRGSIFFTDKNGKKISAATLETSYLLIMNPTLIKSVDEAWQKVSPLV
                     VMDKEKFYQSAAKKGDSYEVLADGLTKETADRILALRLNAIYPIKQRERFYPAGQSAA
                     HVIGLMGYKGDEYAGRYGLEKFYNDTLSREKDLSFA"
     gene            complement(3663..3989)
                     /locus_tag="PLH96_00215"
     CDS             complement(3663..3989)
                     /locus_tag="PLH96_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60234.1"
                     /translation="MKARAIQLDNWGRKITWTAIVGITVCAGLYVYFLSDSVFNTAKR
                     REQIEQAAFLEATVATLESDYLAKLSLINWGYAKTLGFVDSSNSTTYAALDRSAVSMV
                     SLGDEI"
     gene            complement(4028..4918)
                     /gene="rsmH"
                     /locus_tag="PLH96_00220"
     CDS             complement(4028..4918)
                     /gene="rsmH"
                     /locus_tag="PLH96_00220"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPB60235.1"
                     /translation="MHVPVLLQPTIEGLRLQSGETVLDATLGLGGHAKAICEQIGSTG
                     VLIGLDQDPETLELAKRNLEACPGQKMFALGNFRRLSEILQKVGVAQIDAALFDLGFS
                     SFQLDRSGRGFTFQKDEPLLMTLSGDVQSSVLTAAEIVNTWSEASLADIIYGYGEERA
                     ARKIAAAIVTAREENEIRTTFQLAEIIKKALGPRGRFGRIHPATKTFQALRITVNDEL
                     GAFKEGLGAVWEALSVGGRVAVITFHGLEARLVKEFVRTKKEAGELELLVKATKPDRA
                     EVLANPRSRSAQLRVFKKIN"
     gene            complement(4920..5354)
                     /gene="mraZ"
                     /locus_tag="PLH96_00225"
     CDS             complement(4920..5354)
                     /gene="mraZ"
                     /locus_tag="PLH96_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPB60236.1"
                     /translation="MLIGEFKHTIDDKKRISVPANFRKEIGRKVVVTRGLDNCLFLYT
                     LEGWKKVVEQLNTLPMTQGASRGFNRFIFGGAFEADIDTMGRILIPDHLRDFADLKSK
                     VVIVGLNDRVEIWDEKKWKDHIDKVEKQADVLAENLTNIGIL"
     gene            complement(5791..6867)
                     /locus_tag="PLH96_00230"
     CDS             complement(5791..6867)
                     /locus_tag="PLH96_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPB60237.1"
                     /translation="MKKIFYIFLVTAILTPAVSWAQTQKLEFSVWVPYWKKTAATAEV
                     KNHLAQLSTISPFSFEMTADGQIRDAMKIKEAPWTEMITAARKQGVKVIPSIMWSDGA
                     MMDKIFRNRNLREAHIKDIVDLVVAGKFDGIDIDYENKQASTYYGFAAFLRSLSLKLQ
                     ARNKTLVCTIEPRFAPTSKYREVPTDIQYANNYPAINKYCDEIRVMTYEQTTADWKLT
                     NQKIKLGYYQPLADVAWVSKVAVFTSQEINREKMVLGVVNYGYEYEVTDNGSIYNYKR
                     LRSLSYQAAMELAKAEGVTPKRNSAGELSFAYKKDGATRLVWFSDAQAIADKVALAKK
                     LGVKGVALFRLDGESDPKLWRALK"
     gene            complement(7076..8533)
                     /gene="lysS"
                     /locus_tag="PLH96_00235"
     CDS             complement(7076..8533)
                     /gene="lysS"
                     /locus_tag="PLH96_00235"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPB60238.1"
                     /translation="MASLEEIRKERLRKLDLLKQAGFEPYPAKTEITHLLSEGLNRFD
                     ELAAAQVKLTVGGRVMALRVQGALAFFDLFDGTGKLQAFLKKDDTPEKFKLLEEAVDI
                     GDFIQVSGILFTTKRGEKTIAVEDWKMLAKSLRPLPEKWAGLQDEEEKLRKRYLDILT
                     DPETREVIEKRSVFWQTIRQFLLERGFKEVDTPILENMPGGAEARPFVTHHNALDLDV
                     YLRISPELWLKRLLVAGLPKVFEIGRVFRNEGIDAEHLQDYQVMEFYWAYVDYEQGMK
                     LVEELYKEIAQKTFGTLQFKIRDFAVDFGKPWARYDYAEIIKEKTGVDILTADLAMIE
                     KRLQELAVDYDKDGFNLNRAIDNLWKYCRKQIAGPGFLVNVPVMMEPLAKRRADNPNI
                     VERFQVILAGSENGKGFSELNDPQDQLGRFEEQDKLRIAGDEEAQRKDDDFIEALEYG
                     MPPACGFGLSERLFSFLLDKPVREGQVFPLLRPKK"
     gene            complement(8653..9114)
                     /gene="greA"
                     /locus_tag="PLH96_00240"
     CDS             complement(8653..9114)
                     /gene="greA"
                     /locus_tag="PLH96_00240"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPB60239.1"
                     /translation="MAKKTQYLSEEKYHELEAELAHLKIVERKKNAEDLEYAKSLGDL
                     SENAEYHEARERQADVEDRIAQIEYSLKNSQIVKNHHSGQVEVGTTVTVDKNGQAETY
                     QIVGAEEADMAAGKISYESPLGSALLGYKKGEEVAVVTPKGETKYKIVDIK"
     gene            complement(9189..10904)
                     /locus_tag="PLH96_00245"
     CDS             complement(9189..10904)
                     /locus_tag="PLH96_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB60240.1"
                     /translation="MFYRSRLKSRELNPDEVFLDSSNLPEFDNQQFEGRLEKAISRKT
                     ILFFLLICLGIFIIFVLRTGYLQLAKGEYYANRSLNNTLNLLTIFPERGNIYDRNGEE
                     LAWNDQGRKYSDNPAFSHILGYLGYPSPEDMAQELYESKELSGKSGVEKQYNKELAGV
                     KGVKIIEVNARGEVASDHVINQPQSGKDLTLAIDHKVQAKLYEIIGNVVADRGFTAGS
                     GVIVDTQTGEILAMASYPGYDPNAISLGNDPVKINSYFGDSRNPLLNRLVLGLYTPGS
                     VFKPFMAAAALAEGVIEPQKTIYSAGQLEVVNPYNPKLKTIFKDWKAHGAVDMRKAIA
                     VSSNVYFYVIGGGFGDQKGLGIKNIKKYAEMFGLARLTDIDLPSEAVGSIPSVEWKAK
                     NFDGEPWRVGDTYHTVIGQYGVLVTPLQMARAYAAIANGGKLIKPTVRKLASPKEIQI
                     VASLPIEAKDLKIVREGMRQGVLEGTAAGLNTPSVKIAAKTGTAELGVSKEKVNSWVT
                     GFFPYDTPRYSFVVVMEKGPRSNTIGGVFVMRQLFDWMKIYTPEYLEDTDLSLRGEAA
                     NLPHD"
     gene            complement(10922..11179)
                     /locus_tag="PLH96_00250"
     CDS             complement(10922..11179)
                     /locus_tag="PLH96_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60241.1"
                     /translation="MLFRFGAAFILMVSALFFPWWLNLVLLLVFIFSFPNFYEATIGA
                     FLFDVLRQQPETIWPAMSVTAGVILLVYLSELVKRRVVFYH"
     gene            complement(11169..11963)
                     /gene="mreC"
                     /locus_tag="PLH96_00255"
     CDS             complement(11169..11963)
                     /gene="mreC"
                     /locus_tag="PLH96_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPB60242.1"
                     /translation="MTFYSYRKKSRNWQKPVIVLVVILGLLLIGNFLAQKFFPAAASV
                     ASPFGKVADEAGSGWLNFFAFFRTKEDLLRENEKLARENFDLKLALVNRRGLEKENRE
                     LKRLLDLSNQESKKSIVAKVLLTPNVIASGVVTLDVGRKNSEIKPGDLVVYNDKVLLG
                     RVLEVRDYHTKVGLVSTNKAISVLIGKENLPAEATGLGGGNFSITLPKGAEVVKGDAV
                     TAPEYGGYLLGLVDVIKKTNADPFQTILFNAPINIFQIKWVEVYAV"
     gene            complement(11960..13015)
                     /locus_tag="PLH96_00260"
     CDS             complement(11960..13015)
                     /locus_tag="PLH96_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB60243.1"
                     /translation="MWYDRFFKNLTTDVGIDLGTSTTLMYIKGHGIVLTEPSIVAINQ
                     KTGRVVAVGDDASEMIGRTPAHIQAIKPLENGVISNFEVGEEMIACFLRKALSSVGKA
                     YFRPRVVVSVPSDITNVERRAVRDAVMNAGAREVHIVESPMAAAIGINIPVYDPVGNM
                     IVDIGGGTTDIAVISLGGVVRSKNLHIAGEKYNQDIVNYIRDEFKILLGEKTAEEVKV
                     SIGAVLKQPVPGEALIRGRDLVTGLPREVAVTDSDIREAVFNSVVLFVDAVKEVLEST
                     PPEVVSDIMRRGVILVGGGSQVRGLAELLEKEIEIPIHLAEDPGTAVVRGTGVILDDF
                     ESHRDILIDNEDDLPPR"
     gene            complement(13113..14390)
                     /locus_tag="PLH96_00265"
     CDS             complement(13113..14390)
                     /locus_tag="PLH96_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPB60244.1"
                     /translation="MFQSQLFSKTRREDPKDEVSRNAKLLIRAGFIHKEMAGVYSYLP
                     LGLRVLNKINGIIRKEMDALGAVELELTALQNPLLYEKTDRWSGGKVDIWFKTELQTG
                     GELGLGFTHEEAITELLKNHVSSYKDLPRAIYQIQTKFRNETRAKSGILRGREFLMKD
                     LYSFHVSEEDLDNFYDKVEGAYERIFERIGLGDSTYITFASGGVFSKYSHEFQTLSEA
                     GEDLIYLSEGKRLAVNKEVLNDETLSELGLSRDELIEKKSIEVGNIFKLGTRFSQPLG
                     LYFKDEKGESRPVVMGCYGLGPARIMGVIAEIKSDNKGLVWPRAVAPFDIHLVILGKE
                     VELSGDREALTETAKTALEIYEELTASGKEVLCDDRDLSAGEKLADADLLGIPFRLVI
                     SDSAIKKGKFELVDRATGEIEYLSEAELLNKVR"
     gene            complement(14500..15609)
                     /locus_tag="PLH96_00270"
     CDS             complement(14500..15609)
                     /locus_tag="PLH96_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB60245.1"
                     /translation="MTAIIFIIILAILIFTHELGHFSAAKKFGIRVDEFGFGFPPKLF
                     GFKKGETEYSINLIPIGGFVKIFGENPDEESESGPDNTRSMIYKPRYIQAVVLAAGVF
                     LNLVLAWGLFSVSLFIGMPVALADLPAGAQIKEERLLITSVVKDSPADLSGLLAGDTI
                     LSLSNSDAKVVSPTTEEVQDFLQARENRPVEVTYSRGGGEELEEGILTVVPKTGVVGE
                     KAGIGVSMERVGIARLSFFQSIITGLEFTYRMTVVTVVGFVDFVTSLFTGGSQALAEV
                     AGPIGLFGMVADIRQLGLVYLINFTAVISINLAVLNLLPFPVLDGGRLLFLVIEAVKG
                     SRLNPKIANTLNAIGFSLLLLLMAVVAVSDILKII"
     gene            complement(15686..16234)
                     /gene="frr"
                     /locus_tag="PLH96_00275"
     CDS             complement(15686..16234)
                     /gene="frr"
                     /locus_tag="PLH96_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPB60246.1"
                     /translation="MYNFLPFKNHLQEIEEWLGREYLSVRTGKATPQVLDSVIVESYG
                     SKMPIKHIANISIEDAKTLRVTPWDKNQIKDIQTAIEKSNLGLSAAPNSDGLRLSFPA
                     LTEERRKMLMKLVNEKMEEARVSVRKEREKVWGDIQEQEKAGQISEDDKFKAKDELQK
                     IIDGVNNKIAELAERKEKEIFG"
     gene            complement(16352..17032)
                     /locus_tag="PLH96_00280"
     CDS             complement(16352..17032)
                     /locus_tag="PLH96_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60247.1"
                     /translation="MDFLNNSNLHHAYLLVGRPEDFLPKLRLLLSERLGLANLLTSPD
                     YIEESYSLFGIKDSHSLRRRQVLSAFGPNGRFFVLTIDSATVEAQNALLKTFEEPTKG
                     THFFVILRSEDVFLPTLKSRFIVIRSGESRGSQGDDAIVASFLKLSPVARLEYVKKNF
                     IKNKAIGKGEVFRFLDDLERELYGGSRSIGRGNGLKSLAVVSQAKKHLASPRSSLRLI
                     LENLALTI"
     gene            complement(17077..18084)
                     /locus_tag="PLH96_00285"
     CDS             complement(17077..18084)
                     /locus_tag="PLH96_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPB60248.1"
                     /translation="MFIKKLGIDLGTANTLVYAPNKGIVLNEPSVVAVSEPDNKVVAV
                     GTEAKEMVGKAPDNIHVYRPMKDGVIADYRVTEAMLRYYISKALGKLNFFKPDVMVSV
                     PAGVTSTERRAVIEATIRAGAKNAYVVKEPILAAIGAGIPIYEARGHMIVDIGGGTTD
                     VAVISLGGIVASTSVKHAGNKIDQAIADYIKKTFNLAIGDKTAEEVKASIGSAIPVEE
                     ELVYLIKGRDFMTGLPRSVEVRTNEIVRAIDNELKEILRAIKVVFQETPPELAADIID
                     QGITMTGGSSQLRDLSELVYRKTGVKAYLADEALFCVAKGTGIALEHLGTYKKSIISK
                     R"
     gene            complement(18196..19488)
                     /gene="murA"
                     /locus_tag="PLH96_00290"
     CDS             complement(18196..19488)
                     /gene="murA"
                     /locus_tag="PLH96_00290"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB60249.1"
                     /translation="MPEVTDKFIIDGLGGQQTLSGEIVVGGAKNAALKILASAVLFQD
                     GFMTSNVPEIEDVFRMLDLLVNLGVKVNKKDRGVYQLDASAISNKPLWSEIAKRMRAS
                     IVLTGPLLARFGEISFPYPGGCVIGKRPVDLFLSGFEKMGATVTETEGVFWVKAPREG
                     LWGAEIFFKNQSVTATETFLMTAVLAKGKTVLKNCALEPEVASLGEFLNECGAKISGL
                     GTSTITIEGNGLLTGGGHEYKTIPDRIEAGSFIILGALAASDLKVVGCEPKHLDALIN
                     LLRESGVLLEVGENFVRVGSSKKKYQAIDVKTHEYPGFPTDLQAPLAIFLSQANGESF
                     IFETIFEGRLNYLEALSRMGLEAKNLDTHRAIIYGPRKLEGKEIESPDLRAGLAYVLA
                     AIVASGRSVVHNVKYIDRGYEKIEERFQKIGIKIERIK"
     gene            complement(19671..21746)
                     /locus_tag="PLH96_00295"
     CDS             complement(19671..21746)
                     /locus_tag="PLH96_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60250.1"
                     /translation="MFNFKKNFILFLLLFLFVTPFVLGAETDSVQVGLEVISGSEVSG
                     CTDPSASNYSSSATIDDGSCTYGGGPTPPKDVYGCTDEEALNYDSTATVDDGYCQYPS
                     VPNVTNFSVIYRENKNEAFLSWRNPKKDFSLVRIVRLKGEIPNNPSEGILVFQGKGQS
                     AKDSGVATGSLYYYAAFVKNKKGEFSSGAIASLDLRGEEAEEEKEEVEEKEEEKEEEK
                     EQQEQPEQGEPEDKGGRFDGEDGGRSGRETRDPFVGLSLATSTPLSEASWFLRLLQPG
                     EPIKLFNRGETIYIKGGKTLTLEMNYKQVPRVLKTIGVTFVDTENPQDVTAVILRVSA
                     DKKVYRATVPAFKYGGVYEMYIYLIDYNNQTIVPTRGKISVSGTKRPGAELLSLAVEA
                     AAPTVVASGLAVGFFPSLADIFNYLVRLFGYLFGRRKKAKPWGTVYDSVTKRPLDPAY
                     VSIEPVGGGKEITSAITDIDGRFGFLLPAGNYLVKANKTHYQFPSQKLAGQRADELYD
                     NLYFGQTLTTEGEEVINLNIPMDPIGFDWNEFAKTKTDFFRFYSKKEIWRNRIFNMIF
                     SLGFLLSVYTLVFRPSVLNIILTLLYVAVYIFNKLWRSKYKPRRLYKKETGEPLPFSI
                     IRLFLPEINQQVKNVVTDQVGNFYILVRPGSYYYTVEEKLSDGSYQKIYQSEPLELKK
                     GVFATDLRV"
     gene            complement(21748..22500)
                     /locus_tag="PLH96_00300"
     CDS             complement(21748..22500)
                     /locus_tag="PLH96_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60251.1"
                     /translation="MFNLKKIVAAFTIFFASSGLVFGYVSESSNYRMEKDSFNFGGTE
                     NSTSSNYSLSDTLGEFASGDSSGILYAVKAGYRAAAGDYTITISAPENISLAGLTTTG
                     GQSDGSGSWTVTTNDPSGYLLYISAGAAPALQSSGDSFADYTPASVNVPDFVWSVDST
                     DSEFGFTVSGSDVAAAFKNNGSACNVGSEITADRCWYNLSTSDYLIASNNDANNPAGT
                     VTTVKFKAEIGSGKTQTAGSYSADITLTALSQ"
     gene            complement(22493..23359)
                     /locus_tag="PLH96_00305"
     CDS             complement(22493..23359)
                     /locus_tag="PLH96_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60252.1"
                     /translation="MMKLGIKNFCRPVLLVAVVLFPCWALAESFSLRPAKQEVVLNPG
                     ASAQESFTITNNLGRNSYFELKLKNIELNRLGVYPLTDFVNLEDERVFVADGQTKTVY
                     FGINIPTETAPGGAYGFLQVVASPNQGTKGEAGVSAALGSTIFVRVGGPVKEEGKLVS
                     FGILGRQLKFAAPLEWHFSFENSGNIYLNPYGKMILVNRLTGSQTLVDIPPRSVLPGQ
                     TRLFETKTSALGFPGLYSAFLLLNRGYNDEVDMRETSFWFLPWPILLIFSAVFLFFVW
                     VIIFKIIRKKNV"
     gene            complement(23356..24636)
                     /locus_tag="PLH96_00310"
     CDS             complement(23356..24636)
                     /locus_tag="PLH96_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60253.1"
                     /translation="MTEPENQSSLDKGSLTRSLVSDKQTTLLSKTKKNWLGYQLFWRR
                     RALSPAVKADILPAQRPNPWPAVLLGALFVLSLLSTDLLYWDSAVRAGTKIVAMTTNT
                     IGVTYAYITKPLDYTLDKVAYLTKGVEQKNLAAVTANWGELVKRGYSQVTKTLAIGEN
                     QLVGKLEQASRDSSLVLIQKSNSWAMNINGVKEKVAIRRQLLGARALVVLDKVFGVDS
                     GFFASVKRGASQVSKEVKTLPPQVAATWNSASMINLGRMENTLRQIYERWSIRFIKWA
                     ITLDNGLLWFENLPVRLNQKISAIWTATLLKADEVALNWRNFINPSEVSPEAMAEIKK
                     VVAEEISKNLQLQSQSGQVADDTMRASYGAVVVPSTGDPSADEKIKKDLEKQFSDEVS
                     ISFDKSGQAGVITPIFEDGEKGDDYVFLLTPINQ"
     gene            24735..26630
                     /locus_tag="PLH96_00315"
     CDS             24735..26630
                     /locus_tag="PLH96_00315"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60254.1"
                     /translation="MKIVETIPIAKGVFKERLAYFSNQEIKPGMLVAAPLKNKTITAL
                     VASVKEAGDLKAQIKNSDFAMKKLGPIKNKGFFLPEFLASATWSAEYFACSLGQIIKE
                     LTPKIILDNSENIKVTLPKEDAEDVVSEIQASQASEEERWSFYKSLIREAFAKKQSVF
                     WCLPNVNEIKSAQNFIARGIEQYVIILHNKLSKKELLTAWKKAIENDHSVLIIATPMF
                     LSLPRENIKTFIIDKENSSAYKSRVRPFLDFRRFAEDYARRRKVRLIFGDIVLRSETI
                     YRLGQNEITAINPVKYRLYAEIKQVIAKLNKNSEDRVTVGSLSQELGWLIENNKERSE
                     NLFIFSGRRGLAPVVICRDCQTMVTCDVCQTPLVIHKKTPERQTGRMFICHRCNQAVE
                     IDDACEVCGGSRLAVLGYGIEKIEEDIRTLLPEINIFRLDSDSVKNNKKAIEVVQKFM
                     SSPGSVLLGTEMALPYLPNEIENVAIGGIDAFFALPDFRIAEKLFSLLFRLRAKATRR
                     FLIQTGNPEEKVFDYVLGGNLLDFFREEIAERKKLGYPPFKVLLKVSLTGRPETAKRE
                     MKKLADKLEKYKPLVYPTLTGAIKEYEQNLLLKLEPKDWPNPKLSEIIKNLPPSFVVE
                     VDPEKIL"
     gene            26857..27378
                     /gene="def"
                     /locus_tag="PLH96_00320"
     CDS             26857..27378
                     /gene="def"
                     /locus_tag="PLH96_00320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB60255.1"
                     /translation="MKIVQKDDPVLRQKAKEIPLEKIGSPEIKKIIKQMSEALVKEND
                     GVALAAPQIGKSLRLFIISGKLFQKENDKRKPADLVFINPKIRKLSQRKQKLEEGCLS
                     VRWLYGLVSRADKATVEAYNETGQKFSRSGSGLMAQIFQHEMDHLDGTLFVDKAKNLQ
                     RIEPEKMRHGNRI"
     gene            27456..28307
                     /gene="fmt"
                     /locus_tag="PLH96_00325"
     CDS             27456..28307
                     /gene="fmt"
                     /locus_tag="PLH96_00325"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB60256.1"
                     /translation="MDKTFIFWGTDEFSVKVLETLAKRELEPALIVTVPDKPQGRKNI
                     IVPSPAKVWAQTKGIDVLQPSSLRNPDFVEQLKVKRVVFFLVASYGKIIPTDILAIPP
                     KGTLNIHPSLLPKYRGPSPLETAILAGETETGVTIMLVDEEMDHGPILANQKISLRDQ
                     YSFERLRDETAVIGANLFADILPGYLAGSLEPKAQEHSAATYTKKFTRKDGRLDLSAT
                     PTENYRKILALNPWPGAYFIDQHGNRQIKVKVKKAKLENGELIFERVIPEGRKEASWA
                     DYLRGKR"
     gene            complement(28346..28735)
                     /gene="raiA"
                     /locus_tag="PLH96_00330"
     CDS             complement(28346..28735)
                     /gene="raiA"
                     /locus_tag="PLH96_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPB60257.1"
                     /translation="MKTNIKATSLEMTPAIADYIESKISYLDKLMVGYENSLAEVEVG
                     KITNHHKNGEIMFAEINLTIDGKFFRQVVNDNDLYAAIDQAKDKIERDISSYLKKKNH
                     LLRRGGRAVKNFIKGFYPGRKEKGLPS"
     gene            complement(28811..29038)
                     /locus_tag="PLH96_00335"
     CDS             complement(28811..29038)
                     /locus_tag="PLH96_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPB60258.1"
                     /translation="MHIKVRVSVETKKDEVAELAPDTLIVSVRAKPERNQANRRVIEL
                     VAEHLGVAVGQIRIIHGHHNPAKILEIRSQT"
     assembly_gap    29134..29233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29234..29541)
                     /locus_tag="PLH96_00340"
     CDS             complement(<29234..29541)
                     /locus_tag="PLH96_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPB60259.1"
                     /translation="MEQKLKNALAIMALVAMAAVSYSAISYAWSYAKAVEPGSYKSFS
                     VTGEGKVTAIPDVATFTATVISEGGKGLSEIQAENTKKVNKVIDYLKTLAIEKKDIET
                     T"
     gene            29674..>30311
                     /gene="eno"
                     /locus_tag="PLH96_00345"
     CDS             29674..>30311
                     /gene="eno"
                     /locus_tag="PLH96_00345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012517443.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB60260.1"
                     /translation="MFVIKKILAREILDSRGNPTVEADLWLADGSFGRAAVPAGASTG
                     QYEAKELRDGDHGRFGGLGVLRAVKNINEEIMSKLGKAELTQKSLDEQMIELDATTDK
                     SRLGANAILAVSLAFAKAAAQSKRQELYEYFAELSDTKQITMPVPMMNILNGGRHAEG
                     AIDFQEFMIVPIGASDFNEALRFGAETFHALKKILSARGLSTTVGDEGGFAP"
BASE COUNT         8653 a         7545 c         5852 g         8061 t
ORIGIN      
        1 atttcccagg catagttttg ataggaaaat cttacgaaga aaccttggac cgaaacagaa
       61 aaggtcctcg ttggggcacc actcaaaggc aacaagaaat ggaagcaaaa aatttctttt
      121 ttgaagaggg cccccttttt aaaaaagaag caataaagta tggctatccc gtttttgaga
      181 ccgccgacga agcatttaat tattgcttgg agaagttaag ttagcagttg tcttgcttag
      241 ggtcattaag tgcgcttcgc gctcaaaatt gttgtggtcc acaataattc actcgtcagt
      301 cactcatgaa ttttccagac ccggactcag ctgttttttc tactgaaaaa acatgcttcc
      361 gtcttcacac ttcgcccgca agcaaagcag tttgcttgct tttcgctcgt gtgaccctac
      421 ggggagtctc ccgcggctct cgtcgcgcca gtcgcgctcc tcggcctggg caccagctcg
      481 ctagattttt ctgctgaaaa atcatcgctc cgctgttcta ctccccgacg cgagtgaagc
      541 ccgtccgaac ggattcatcc gggcgggcag ttcactcact tagggtcgct taagaaaaat
      601 ggctcaccaa aagtgggctc atttttctgc aagtgaccct acggggagtc gaaccccgat
      661 taccaccgtg agagggtgat gtcctagcca ttagacgata gggccggaaa aggatttatg
      721 gattatatcc gcaagtccgg ataaatccag gtactggcat cgcattttac tataccacgc
      781 aacgtttttt gaagcaatca gcccaaaagc cagggcgagc gcctaaagct tggatttttg
      841 tctcaatgtt gcgttagagc cactccgctt cttgtctcac cagcaatttt tcttttttat
      901 ccggttccgc cataatttct accactgttt tttccattcg cattgattga gagcctttga
      961 ccaaaatcac gtcccctgcc tctatttcgt tctgcaaagt ctgaccagcc gataacgaat
     1021 catcaaaatg cctcactttt tcacccaagc ctttttcaat gaaaacttcg tcaataaatt
     1081 tagaccgcaa gccgaccgtg taaaccaggt cacaaacttc cgctgccaga taaccaattt
     1141 cccggtgcgc ttccggcgcg taggtaccaa gctgaagcat atcgcccaaa accgcaatcc
     1201 gccggccctt gacctgcaaa aggcttagcg ttttcagccc cgccgccacc gccgccggcg
     1261 acgaattgta agtgtcgtcc aagactaccg aattttttaa accaggcaaa actcgcaaac
     1321 gtccggccgc cggtttgtaa tcttttaaaa cttcaatcat ttcaattacg ttcaccccta
     1381 aaatatctcc caacgccaaa gctgccaaga ctggataaat ttgatgctcg ccgacaaccc
     1441 cgccaactcg cactggcaaa ctcttgccgt tgtgttcaaa tttgaaagtc aacccgctcg
     1501 gcaaaccggt ttcgtcatac aaaacaccct gggcttcagc gcgataatca gccaaatcat
     1561 taaaaccaaa agtcaggatt tcgcccttga tgcctccaag atgagcggca acatttttgt
     1621 catcggcatt aattaaaact ttgccctggg ctgggactgc cttggccaaa agcatttttt
     1681 ctttgatcac ttcggccggt gaagaaaaga actctacgtg caccggcata tccggcaaaa
     1741 aagtaacaac cgcgtggtca aatttcaccc atttgacaat ttgcgcgatt tcacccggat
     1801 attccacgcc aatctcaagc accaaccatt ttgggtaatc ttttttaaaa ataattaaaa
     1861 acaaaccgtg gaaaaaaata tccaaccaac ccaagatgct ataccaagcc gattgacaac
     1921 ccagaatcgt cagggccacg ccgatatcgg cgctgttgta actcttctcg cttttagaca
     1981 ccgacaaata attttttgtt acttcataaa tcgcgtcctt ggtggaggtt ttgccaacac
     2041 tgccagtcac tccaataatc tttggtttat actttttgag caccaatttg gcctcccagc
     2101 gcaagataac gacaacgact ggccgaaagc cctttttgag catttttaag gtgtttttca
     2161 tcaattttga attattgggg gtctctgttg ttagcggtct ggtggcactt ggtaatagtt
     2221 taacaaaaat ttggtcaaat tgctaaacgg ttcagtcaaa gtttccgatg agtaacgtac
     2281 gcctttgggg tctttaatat acataaaaat gataaaacgc gggttgaaag ctgggaaata
     2341 accaaagaaa gtgtgcaggt aacggtcagg ataatatttt ctggtttccg ggttcaccat
     2401 ttgcgccgtg ccagttttgg ccgcaaccga ataatgttcc agcttagctt tgccgccggc
     2461 caatttctcg tctacgactt ccaccaacat ccggctaatc tcctcgcttg tctctttttt
     2521 tatcacttgc cttataatct tgggttcaat cttgcgcgat aatttatttt ggtaattgat
     2581 ttctttgaca atatgaggct caatcagctt accaccgtta ccaagcgcgg ccaacgcgcg
     2641 cgcggtcgca atcggcgtaa aagacaagcc ctgaccataa gccgctgtga cgtaattaat
     2701 ttcataaggt tcatagattg tttgaattcg attgttcaat tcccccggca agtcaatgcc
     2761 ggttttttcg ccaaagccaa aggcttcata gtaggcgcgg aatttttctt ggcccagttg
     2821 acgagccaca aaagccatcc ccacgtttaa agattggttc aaaacttctt gcattggcac
     2881 tactcctctg gctctgccgt cataattgca gatttttttg ccactgaccg taatgcagcc
     2941 agtatcgtta aaagtagttg aggttgtgac tgcgccggcg tccaannnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnggcaa acgataaatc tttttctcgc gacaaagtat
     3121 cattataaaa cttctccaag ccgtaacgtc ccgcatattc gtcccccttg taacccatta
     3181 ggccaatcac gtgcgcggcg ctctggccgg ccgggtaaaa cctctccctc tgtttgatgg
     3241 gataaatcgc attcaaacgt aaggctaaaa tccggtcagc ggtttctttg gtcaagccat
     3301 cggccaaaac ttcataagaa tccccttttt tggccgctga ctggtaaaat ttttctttat
     3361 ccataaccac caagggcgaa actttttgcc aagcttcatc cacggatttg attaaggtcg
     3421 gattcataat taacaaatat gacgtttcca aagtcgccgc ggaaattttt ttgccatttt
     3481 tgtctgtaaa aaaaatagag cctcggtcgt atattctggc cgaacccgga ctaaaatgct
     3541 gtttatccgc ctccgcgcga taatggtcgg ccttgaccac ttgaataaaa aataagcgta
     3601 caataaataa agccgccaag gcaaaaatac caaatgccaa aaatttaacc ctgagaccgg
     3661 agttatattt catcacccaa tgaaaccatg ctgaccgctg agcgatcaag cgcggcgtaa
     3721 gtcgtgctgt tagacgaatc aacaaaacca agtgttttag cgtaccccca gtttatcaaa
     3781 cttagtttgg ccaaataatc cgattccaac gtggcgacgg tcgcttccaa aaaggctgct
     3841 tgttcaattt gttctcgacg cttagctgta ttaaaaaccg agtcactcaa aaaatagaca
     3901 taaaggccgg cgcaaacggt gataccaaca attgccgtcc aagttatttt tcgaccccaa
     3961 ttatcaagtt gtatggctcg cgctttcatt tgtttataaa aattttttga taatgtataa
     4021 ctgatgttta attaattttt ttgaaaaccc tcaattgagc gctgcgtgaa cgcgggttgg
     4081 ccaaaacctc cgcgcgatcc ggttttgtgg ctttgactaa aagttccaac tcccccgctt
     4141 ctttttttgt cctgacaaac tccttgacca atctggcctc tagaccatga aaagtaatga
     4201 ccgcgacccg accgccaacc gacaaggcct cccaaaccgc tcctagacct tctttgaacg
     4261 cgcccaattc gtcattaacc gtaatccgca aagcttgaaa agtcttagtc gccggatgaa
     4321 ttcggccaaa ccgaccgcgc gggccgagcg cctttttgat gatttcggcc aactggaaag
     4381 tggttctgat ttcattttct tctctggcgg tcacaatggc ggccgcaatt ttccgcgcgg
     4441 cgcgttcttc accatagcca taaatgatat cggccaaact cgcttccgac caagtgttga
     4501 caatttccgc agctgtcaaa acactggact gaacgtcacc actcaaggtc atcagcaacg
     4561 gttcatcttt ctgaaaagta aaaccgcggc ccgaccggtc cagttgaaac gaactgaaac
     4621 cgaggtcaaa caatgctgca tcaatttggg cgaccccaac tttttgtaaa atttcagaca
     4681 aacgacggaa attgcctaag gcgaacattt tctgacccgg gcaagcctcc aagtttcgtt
     4741 tggctagctc aagcgtctca gggtcttggt ccagcccaat caaaacacca gttgatccga
     4801 tttgctcaca aatagcttta gcatgaccac ctaaaccaag cgtggcgtcc agaacggtct
     4861 cgccgctttg caaacgcaat ccctcaattg ttggttgtaa aagaacagga acgtgcatac
     4921 tacaagattc cgatatttgt cagattctca gccaaaacat cagcttgctt ttccacctta
     4981 tcaatatggt ctttccactt cttttcgtcc cagatttcta ctcggtcgtt taagccaacg
     5041 ataaccacct tgctctttaa atcggcaaag tctcgcaagt gatcaggaat taaaattcgc
     5101 cccatagtgt ctatgtcggc ctcaaaagcg ccgccaaaaa taaaacgatt aaaaccgcgc
     5161 gaagcgcctt gagtcattgg caaagtgtta agttgttcca ccactttttt ccagccttca
     5221 agtgtgtaga ggaaaagaca attgtccaat cctctcgtga ctacaacctt acggccaatt
     5281 tctttgcgaa agttggccgg aacggaaatc cgttttttgt cgtctatggt gtgtttgaat
     5341 tcgccgataa gcataatctt tgagcgtgaa acgaagcaaa ctcccctttt tttctaaatc
     5401 aaatttttaa gaaaaaattt gatttgataa gcatttttac ctgaaaatgc attgacccac
     5461 ttcatcccac cacatagaca ttttatacca ccggacacca cttgggcaaa taaaatgttg
     5521 tggataactg ctaaaattac ctctttgaac ccaaaaggcg tgaaacacaa ggctaaaaac
     5581 agcaacaaga attcactgta acttaataat caaccgggga tgtttgcaat tttttaaaga
     5641 aaaaaccgaa acagtgactc tgctcaaaaa ttccgtttgg gaaaatttaa aagctctgac
     5701 tctaggagtc agagctttta aattttgatt ctcccccatt cattcatttc atactcatct
     5761 ctgcccattg cgctggctgg aaattttaac ttattttaac gcgcgccata gttttgggtc
     5821 gctttcgcca tcaaggcgga aaagagccac tcctttcact ccgagctttt tggccaaagc
     5881 cactttgtca gcaatcgctt gcgcatccga aaaccacact agcctcgttg cgccgtcttt
     5941 cttataagca aaggataatt caccggcact gtttctcttg ggagttaccc cttcggcctt
     6001 ggccaactcc atggcggcct gataacttaa gctccgcaaa cgtttgtaat tataaattga
     6061 accattatcc gtcacttcat attcatatcc gtaattgacc actcctaaca ccatcttttc
     6121 tcggttgatt tcctggctgg taaaaacggc caccttgctc acccaggcca cgtccgccaa
     6181 gggctggtag taacccaatt ttattttttg gttggtcaac ttccagtcag cggtagtttg
     6241 ttcataagtc attacccgaa tttcatcaca atacttattg atcgccgggt aattgttggc
     6301 gtattgaata tcagtcggca cctcacgata cttggaagtc ggcgcgaagc gcggttctat
     6361 ggtgcaaact aaagttttgt ttcgggcctg caatttgagc gacaacgaac gcaaaaaggc
     6421 ggcaaaccca taataagtgg aggcctgctt gttctcataa tctatgtcaa taccgtcaaa
     6481 cttgcccgct accactaaat caacaatatc tttgatatga gcctcgcgca aattgcgatt
     6541 acgaaaaatt ttgtccatca tcgcgccatc agaccacata attgagggaa taactttcac
     6601 tccctgcttt cgcgcggccg taatcatttc cgtccaaggc gcctctttga ttttcatcgc
     6661 gtcacgaatc tggccgtcgg ctgtcatttc aaacgaaaaa gggctaattg ttgaaagttg
     6721 cgctaaatga tttttaacct ccgcggtcgc cgccgtcttt ttccaataag gcacccaaac
     6781 cgaaaattcc aatttttggg tctgagccca agaaacagcg ggggttaaaa ttgccgttac
     6841 taaaaaaatg taaaatattt ttttcataat taaatggtca acaataaatg ttcgcttatt
     6901 gtttagaacg aataagaaca acaggcgtga ccataaattt acaggtccac ggtggagacc
     6961 agcgcctaaa ttaaagatga atggaaattg cctcaaagtt cccttccctc actccctcac
     7021 gttagcgcca atgaaaagaa ttgtgcggac actctctcag gttgattttt gaaatttact
     7081 tcttcggacg aagcaaggga aaaacctggc cttctctgac cggtttatcc aaaagaaaac
     7141 taaataatct ttcgcttagg ccaaaaccac aagctggcgg cataccatat tccagcgctt
     7201 caatgaaatc atcatcttta cgttgggctt cttcgtcacc ggcaatgcgt agtttgtcct
     7261 gttcttcaaa gcgaccaagc tggtcttgcg gatcattcaa ttcgctgaac cctttgccgt
     7321 tttccgaacc ggctagaata acttgaaagc gctccacaat atttgggttg tcagcgcgac
     7381 gtttggccaa cggctccatc ataaccggca cgttaaccag aaagcccggt ccagcaattt
     7441 gtttgcgaca gtatttccaa agattatcga ttgcccgatt caggttgaaa ccatctttgt
     7501 cataatcaac cgcaagttct tgcagtcttt tctcaatcat cgccagatca gctgtcagaa
     7561 tatcaacccc cgttttttct ttgatgattt cagcgtagtc ataacgcgcc caaggtttgc
     7621 caaagtctac cgcaaaatct ctgattttaa attggagtgt gccaaaagtt ttttgggcaa
     7681 tctctttata aagttcttcc actaatttca ttccctgttc ataatcaacg taagcccaat
     7741 aaaattccat tacttggtaa tcttgcaggt gctcggcatc aatcccctca ttacggaaaa
     7801 ctcgtccgat ttcaaacact ttgggcagac cagcgaccaa aagacgttta agccaaagtt
     7861 ccggcgaaat tcgcaaataa acatccaaat ccaaagcatt gtgatgggtc acaaaaggcc
     7921 tcgcttcggc gccaccgggc atattttcca aaattggcgt gtcaacttcc ttgaaacctc
     7981 gctcaagcaa aaactgacgg atggtctgcc aaaaaacaga acgcttttca ataacctcgc
     8041 gtgtttcagg gtcagtcaaa atatctaaat agcgtttgcg taatttttcc tcttcatctt
     8101 gcaaaccggc ccacttttca ggcaaaggac gcagactttt agcgagcatt ttccaatcct
     8161 ccaccgcgat cgtcttttca cctcgtttgg tcgtgaataa gatacctgag acttggatga
     8221 aatcgccaat atcaaccgcc tcttccaaca atttaaattt ttctggcgtg tcgtcttttt
     8281 tcaaaaacgc ttggagcttg ccagtgccgt caaataaatc aaaaaaggcc aatgcgccct
     8341 gaacacgcaa cgccatgacc cgtccaccaa cggtcagttt gacctgtgcc gcggctaatt
     8401 cgtcaaaacg atttaagcct tctgacaaca aatgcgtaat ttccgtcttg gccggataag
     8461 gttcaaaacc ggcttgcttg agcaagtcca acttgcgcag gcgttctttg cgtatttctt
     8521 caagagaagc cataaattaa gttaaaaatt agaaattata agttaaaaac caagaaagct
     8581 gacataaaaa tagtatagca aaaagcggca aaaattgaag tccaaaatta agagcggaaa
     8641 tttttccgac ttttatttta tgtctactat tttgtacttg gtttcacctt ttggagttac
     8701 gaccgcaacc tcctcgcctt ttttatagcc aagcaaagcc gagcccaagg gcgattcata
     8761 agaaattttg ccggctgcca tgtcagcttc ttccgcgccc actatttggt aggtttccgc
     8821 ctgaccattt ttgtcaactg ttaccgttgt gccaacctcc acctgaccgc tatgatgatt
     8881 tttgacaatt tgcgaatttt tgagagagta ttcaatctgc gcaatccgat cttccacatc
     8941 agcctgtctt tcgcgcgctt cgtgatattc ggcattttcc gacaaatcac caagcgactt
     9001 ggcatattcc aaatcctcag catttttttt gcgttccact attttcagat gagccaattc
     9061 cgcctcaagt tcatgatatt tttcttcact caaatattgc gtttttttag ccatattatg
     9121 acaataatag cccaagttca tggaaaaatc aatgggcaat acaatgcgaa tatcacgaat
     9181 gagaggcatt aatcgtgggg aagatttgcc gcttcgcccc ttaaggaaag gtcggtatct
     9241 tccaaatatt ccggagtata aattttcatc caatcaaata attgtcgcat gacaaagacc
     9301 ccgccgatcg tattactgcg cggacctttt tccatcacga ccacgaacga ataacgcggg
     9361 gtgtcataag ggaaaaaacc agtcacccaa gaattgacct tttctttgct tacccccaat
     9421 tcagcggtac cagtcttggc ggcaattttg acagacggtg tgttcaagcc ggccgccgtc
     9481 ccctccaaca cgccctgcct catcccctct ctgactatct ttaaatcttt ggcttcaatc
     9541 ggcagggaag ctactatttg aatctctttt ggcgaagcca gtttccgtac ggtcggtttt
     9601 atcagtttgc caccgttggc aatcgccgcg taagccctgg ccatttgcaa aggcgttacc
     9661 aaaacgccat actggccaat tactgtgtga taggtatcgc cgactcgcca cggctcgccg
     9721 tcaaaattct tggccttcca ttcaacactc ggaatagacc ccaccgcttc actgggcaag
     9781 tcaatgtcgg tcagacgagc caagccgaac atctcagcat attttttaat gttttttata
     9841 cccaaaccct tctgatcgcc aaagccgcca ccaattacat agaagtaaac gtttgatgaa
     9901 acggcaatgg cttttcgcat atccacggcg ccgtgcgctt tccaatcttt gaaaatcgtt
     9961 tttaattttg gattataagg attgaccact tcaagttggc cggcgctata aatggttttt
    10021 tgcggttcaa tgaccccttc cgccaaagcg gccgcggcca taaacggctt aaaaaccgac
    10081 cccggcgtat acaatccgag aaccaaacgg tttaagagcg ggttacggct gtcaccgaaa
    10141 taagaattta tttttaccgg gtcgttgcca agcgaaatgg catttgggtc ataacccggg
    10201 tagctggcca tcgccaaaat ttcaccagtt tgagtatcaa caataacccc gctaccggcc
    10261 gtaaaaccac gatcggccac cacattgcca attatttcat aaagtttagc ttgcactttg
    10321 tggtcaatgg ccagggtcaa atccttgccg ctttgcggct ggttaatcac atggtcagaa
    10381 gccacttccc ctcgtgcatt cacctcaata atttttaccc ccttaacacc cgccaattct
    10441 ttgttgtatt gcttctccac gccacttttg ccggacaatt ccttagactc ataaagttct
    10501 tgggccatat cctccggcga aggatagccc aaataaccaa ggatatgact gaaagctgga
    10561 ttgtcgctat attttcgacc ttggtcgttc caagccaact cttcgccgtt gcggtcgtaa
    10621 atgtttccgc gttccggaaa aatcgttaac aagttcaggg tattattcaa gcttcggttg
    10681 gcgtagtatt cacccttggc caactgaaga tagccggttc gcaaaacaaa aataataaaa
    10741 atccccaaac aaatcaaaag aaaaaaaaga atggtctttc ggctaatcgc cttctccaaa
    10801 cggccttcaa actgctggtt atcaaactcc ggcaaattgc ttgaatcaag gaaaacctca
    10861 tcagggttaa gctctcgtga tttgagacgt gaacgataaa acatatttaa ataataaccg
    10921 attaatggta gaaaacaact cgccgcttca caagctcgct caaataaact aacaaaatca
    10981 ctccggcggt aacggacatc gccggccaaa tagtttctgg ttgttggcgc aagacatcaa
    11041 acaaaaaagc gccgatcgtt gcttcgtaaa aatttggaaa gctaaaaata aaaaccaaaa
    11101 gcaaaaccaa gtttaaccac cacgggaaaa aaagagctga aaccatcaag ataaaggcgg
    11161 cgccaaatct aaacagcata aacctcaacc cacttaatct gaaaaatatt tatcggcgcg
    11221 ttgaaaagaa ttgtttggaa aggatcagca ttggtttttt ttatcacatc caccaacccc
    11281 aacaagtaac ctccatactc aggcgcggtc accgcgtcgc ccttgaccac ctcagcgccc
    11341 ttaggcaaag tgatagaaaa gtttccgccg cccaaaccag tggcttccgc cggcaaattt
    11401 tctttgccaa tcaaaaccga aatggcttta ttggtagaaa ctagtccaac tttcgtgtgg
    11461 taatcccgca cttccaaaac tcggcccagt aaaaccttat cgttgtaaac caccagatcg
    11521 ccgggtttaa tctcagagtt ttttcgtcca acatccaatg tcacaacccc gctcgcaatc
    11581 acgtttgggg tcaacaagac tttggccaca attgattttt tactttcctg attagaaaga
    11641 tcgagcaatc tttttaattc acggttttct ttttccaatc cccgacgatt gaccaaggct
    11701 aattttaaat caaaattttc gcgcgccagt ttttcgttct cgcgaagaag gtcctctttg
    11761 gtccgaaaga aagcaaaaaa gttgagccag ccagacccgg cttcatcagc caccttgcca
    11821 aatggcgaag caacgctggc cgccgctgga aaaaactttt gcgccaaaaa attacctata
    11881 agtagcaagc ccaaaattac cactaaaaca atcaccggtt tttgccaatt tcgactcttt
    11941 ttgcggtaac tataaaaagt catcttgggg gtaaatcatc ttcattatca ataagaatgt
    12001 cgcgatgtga ttcaaagtcg tccaaaataa caccagtacc ccttaccacc gccgttcccg
    12061 ggtcttccgc caaatggatt ggtatctcaa tctctttttc caagagctcg gccaagcctc
    12121 tcacctggga accgcccccg accaaaataa ccccccgacg cataatatct gaaactactt
    12181 ccggcggggt agattccaaa acttccttta ccgcgtccac aaacaaaaca actgaattga
    12241 atactgcctc gcgaatatca gagtcggtca ccgccacttc acgtggtaaa ccggttacca
    12301 ggtctcgtcc gcgaatcaaa gcttcacccg gcactggttg cttaaggacc gcgccaatgg
    12361 aaacttttac ttcctcggca gttttttcac caagcaaaat tttgaattcg tcgcgaatat
    12421 aattcacaat atcttggtta tatttctcac cggcgatatg taaatttttg gaacgcacaa
    12481 cgccacccag cgaaatgaca gcaatgtcgg tggtgccacc gccaatatcc acaatcatgt
    12541 taccaaccgg gtcatagacc ggaatattga tgccaatggc cgcggccatc ggcgattcca
    12601 caatatggac ttcgcgcgcg ccggcgttca tcaccgcgtc tcgcactgcc cgccgttcca
    12661 cgttggtgat gtctgaaggc acactcacca ccacccgtgg tcgaaaatat gccttcccaa
    12721 ctgaactcag ggctttgcgc aaaaagcaag caatcatttc ctcacccacc tcaaaatttg
    12781 aaataacacc attttccagc ggcttaatcg cttgaatgtg cgccggggtg cgaccaatca
    12841 tttcactggc atcatcccca accgccacca ctcggccagt cttttggttg atcgccacga
    12901 ttgatggttc cgtcaaaaca atcccatgac ccttgatgta catcaaggtc gtagacgtgc
    12961 caaggtctat ccccacatca gttgtcaaat ttttaaaaaa tctatcgtac cacatcttgc
    13021 aataggaatt agaagttgag aactaaaata aattataagt ttgtttgtaa tttaacagaa
    13081 cctgactttt taatttttaa tctagactta acttaccgaa ctttatttag caattccgct
    13141 tccgataagt attcaatttc ccccgttgct cggtcaacta actcaaactt ccctttcttg
    13201 atcgctgaat cgctaatcac caaacgaaaa ggaatgccga gcaaatcggc gtcggccaac
    13261 ttttcaccag ccgacaaatc gcggtcatca cacaaaacct ctttcccgct tgccgttaac
    13321 tcctcataga tttccaaggc tgtcttggcg gtctccgtca aagcctcgcg gtcacccgac
    13381 aattcaactt ctttccccaa gattaccaga tgaatatcaa acggcgccac ggcgcgtggc
    13441 cagaccaaac ctttattatc agatttgatt tcggcaatca cccccattat gcgcgctggg
    13501 ccaagaccgt aacagcccat cactaccggc cgtgactctc ctttttcatc tttgaagtag
    13561 agaccaaggg gctgggaaaa gcgagtccct agtttaaaaa tgttgccaac ttcaatggat
    13621 tttttctcga ttagttcatc gcgagaaagg cctaattcgg acaaggtttc gtcattcaaa
    13681 acttctttat taaccgccag ccttttcccc tccgacaaat aaatcagatc ctcgcccgct
    13741 tcgctcaaag tctgaaactc gtgactatat ttactaaaga caccgccgga cgcaaaagtg
    13801 atataggttg agtcacctaa accaattcgt tcaaaaattc tttcataagc gccttcgacc
    13861 ttgtcataaa aattgtccag atcctcttcg gaaacatgaa aggaatagag atctttcatc
    13921 aaaaattcgc gcccgcgcaa aataccgctc ttggcgcgag tctcattgcg aaacttagtc
    13981 tgaatttgat aaatggcacg cggtaaatct ttatatgaac taacgtgatt ctttagcaat
    14041 tcggtaattg cctcttcgtg tgtaaaacca agacccagct cgccaccggt ctgcagctca
    14101 gtcttaaacc aaatatccac cttgccgccg ctccaacggt cagtcttttc ataaagcaac
    14161 gggttctgca gagcggtcaa ttcaagctcc acggctccca aggcgtccat ctctttacga
    14221 atgatgccgt ttattttatt cagaaccctc aagccaagcg gcaaataaga ataaacgccc
    14281 gccatctcct tatgaataaa accggcccga atcaacaatt tagcatttcg ggaaacttcg
    14341 tccttagggt cttcccgcct agtttttgag aataattggg actgaaacat atgctcatct
    14401 taaaccaaaa aaagaggttc gtaaagaaac cgcctcaaat attctggaac agtcaagtcg
    14461 cttgacacaa tcccaggatg ttggtttata acgttgacct taaattattt tcaaaatgtc
    14521 gctcaccgcc accacggcca tcaagagcag caacaaagag aaaccgatag cattcaaggt
    14581 gttggcgatc ttagggttga gacgtgagcc cttaaccgct tcaataacca aaaaaagcaa
    14641 gcgaccgcca tccagaaccg gaaacggcaa caagtttaga accgccaagt tgatagaaat
    14701 aacggcggtg aaattaatca gataaactaa accgagttgt ctgatatcag cgaccatccc
    14761 aaacaaacca atcggaccgg ccacctccgc caaagcctga ctgccacccg taaacaaact
    14821 ggtgacaaaa tcgacaaaac caacaactgt cacaaccgtc atgcggtaag taaattcaag
    14881 accagtgata attgattgga aaaaagacaa gcgcgcgata ccgactcgtt ccattgagac
    14941 accgatgccg gccttttcgc caaccactcc cgtcttaggc accaccgtta aaatcccctc
    15001 ttccaattct tcgccgcctc cccgagagta agtaacctct accggacgat tttccctggc
    15061 ttgcaaaaaa tcctggacct cttcggtggt cggcgaaacg actttcgcgt cactattaga
    15121 aagcgataaa atagtgtccc ccgccaatag gcccgacaag tctgctggtg aatcttttac
    15181 cacgctggta atcaaaagcc tttcttcttt tatttgcgcc ccagccggca aatcggccaa
    15241 agcgaccggc ataccaataa aaagactgac cgaaaaaagc ccccacgcca agaccagatt
    15301 caaaaagaca ccggcggcca agacaacagc ctgaatataa cgcggcttgt aaatcatact
    15361 ccgcgtgttg tccgggccac tctctgattc ttcatccggg ttctcgccaa aaatcttgac
    15421 gaagccgcca atcggaatca ggttgattga atactccgtc tcccccttct taaagccaaa
    15481 cagttttggc ggaaaaccaa aaccaaactc gtccaccctg atgccaaatt ttttggcagc
    15541 cgaaaaatga cccagttcat gagtaaaaat caaaatcgcc aaaataatga tgaagataat
    15601 agcggtcata ttgttaagtt aaaaatcaga aattaaagct aaaactttct aaaacaaagc
    15661 gaggaaaata caaataatac ctcaactaac caaaaatttc tttttctttg cgttcggcca
    15721 gttcggcaat tttattgttc acgccatcaa taattttttg caattcatct ttggccttaa
    15781 atttatcgtc ctcagaaatt tgaccggcct tttcttgctc ttgaatgtcg ccccaaacct
    15841 tctcccgttc tttgcggacc gacactcgcg cttcttccat tttctcattt accaacttca
    15901 tcaacatttt ccgcctttct tcggtcaaag cgggaaatga caaacgcaaa ccatcggaat
    15961 ttggcgccgc cgataatccc aaattggact tttctatcgc cgtttggata tcttttattt
    16021 ggttcttgtc ccacggcgta actctgaggg ttttggcatc ctcaatggaa atgttggcaa
    16081 tatgtttaat cggcattttt gaaccataag actcaacaat gacgctgtct aaaacctgtg
    16141 gcgtggcctt gccagtccgc acgctcaaat attcgcgacc aagccactct tctatctctt
    16201 gtaaatgatt tttaaatggc aaaaaattat acattgatac aaagttagct cttaattaag
    16261 ttaataatag accaaaaaat aacaagcgta aagccgaaat caaaaaagat tcagggaaac
    16321 ttcaaagtta gcacttgagt tattaaaatc cttaaatcgt caacgccagg ttttccaaaa
    16381 tcaaacgaag tgaagatcgc ggactggcaa gatgtttttt ggcttggcta acgactgcca
    16441 aactttttag accatttcct cgtccaatag accgggaacc tccatacaac tctctctcca
    16501 aatcatccaa aaaacgaaaa acttctcctt tgccgattgc cttgtttttg ataaaatttt
    16561 ttttgacata ttccaggcgc gcaaccggag aaagtttaag aaaactggca actatcgcgt
    16621 catccccttg gcttccgcgc gactcgccgg aacgaatgac aataaaacga gatttcaatg
    16681 tcggcaaaaa aacatcttct gagcgtaaga taacaaaaaa atgtgtcccc ttggttggct
    16741 cttcaaaagt cttgagaagc gcattctgcg cctcaaccgt ggccgaatct atcgtcaaaa
    16801 caaagaaacg tccgtttggg ccaaaggcgc tcaaaacctg gcgccgtctg agggaatggc
    16861 tatccttaat tccaaataaa gaataacttt cttcaatgta atctggacta gtcaagagat
    16921 tggccagccc taatcgttcc gaaagtaaca aacgaagctt gggcaaaaag tcctctggtc
    16981 gtcccaccaa aagataagcg tgatgtaaat tggaattatt taaaaaatcc atttagcatt
    17041 ttaaaaacga gaacataaaa atcaagaact gaaagattaa cgtttactga tgatactctt
    17101 cttataggtg ccaagatgtt ccaaggcgat gccggtccct ttggccacac aaaaaagcgc
    17161 ttcgtctgcc aaataagctt tgaccccggt cttgcgataa accaattcag acaaatcgcg
    17221 aagttgtgat gaaccgccag tcatcgtaat tccctggtca atgatgtcag cggccagttc
    17281 gggcggagtt tcttgaaaaa ccaccttgat cgcccgaaga atctccttta attcgttgtc
    17341 tatcgcccgc acaatttcat tggtccgcac ttccaccgaa cgcggcaacc cggtcataaa
    17401 atcacggcct ttgattaagt agaccaattc ctcttccacc gggatagccg agccaataga
    17461 agcttttact tcttcagcgg tcttgtcgcc aattgccaaa ttgaaagtct tttttatgta
    17521 gtcggcaatt gcctggtcaa ttttattgcc agcgtgtttg accgaagtgg aagccactat
    17581 cccgcccaac gaaataaccg ccacatcagt ggtcccgccg ccaatgtcta caatcatatg
    17641 accacgagct tcataaatcg gaataccggc gccgatggcc gccaatatag gctctttgac
    17701 cacataagcg tttttagcgc cggcgcgtat agtcgcctct atcaccgctc gccgttcggt
    17761 ggaagtaacc ccagccggca cggacaccat cacatccggc ttgaaaaaat ttagtttccc
    17821 caacgccttg ctgatgtaat aacgaagcat cgcctcggta acacgataat cggcgatcac
    17881 tccatccttc atcggcctgt aaacatgaat gttgtcaggc gctttgccaa ccatttcttt
    17941 ggcttcagtc ccaacagcaa caactttatt atcgggttca gacaccgcca ccactgaagg
    18001 ttcattcaaa acaatcccct tatttggcgc ataaactaag gtgttggccg tccctaaatc
    18061 tatccctaat tttttgataa acatcgccac gacaataaat gtaaaaacta aagctaattg
    18121 tcctcatttt agcccaaaaa atgagaaata accacttgac gtaaagtggt aaatataata
    18181 taaaaataac caattttatt ttattctttc tattttgatg ccaatttttt ggaaacgttc
    18241 ttcaattttt tcataaccgc ggtcaatata tttgacgtta tggacgacgg accgaccaga
    18301 agcgacaatc gccgctaaaa cataagccaa gccggcgcgc aggtcaggac tttctatttc
    18361 cttgccctct agtttacgcg gtccataaat aatcgcccgg tgagtgtcta aatttttagc
    18421 ttctaaaccc attcgcgaca atgcttctaa ataatttaaa cgcccttcaa aaatagtttc
    18481 aaaaataaaa ctctcgccgt tcgcttgaga caagaaaatt gcgagtgggg cctgcaaatc
    18541 agttggaaaa ccagggtact catgagtctt cacatctatc gcttggtatt ttttcttgct
    18601 acttccgacc ctgacaaagt tctccccaac ttcaagcaac accccgcttt cacgcaataa
    18661 gttgatgaga gcgtccaagt gctttggttc acaaccgacc actttcagat cagaggcggc
    18721 caaagcccct aaaataataa aactgccggc ttcaatccga tcagggattg ttttgtattc
    18781 atgtccgccg ccagtcaaaa gcccattgcc ttcaattgta attgttgacg tgcccaaacc
    18841 cgaaattttg gcgccgcact cgtttaaaaa ttcacccaaa ctggcaacct ccggttccaa
    18901 agcgcaattt tttaacactg tcttgccttt ggccaaaacc gcggtcatta gaaaagtttc
    18961 cgtggccgtg acactttgat ttttgaaaaa tatttcagct ccccaaagac cctctcttgg
    19021 cgctttgacc caaaaaacac cttccgtttc cgttacggtc gcgcccatct tttcaaagcc
    19081 agacaaaaat aaatcaaccg gccgtttgcc gatcacgcaa ccacccggat aaggaaacga
    19141 tatttcgcca aaacgcgcca acaagggtcc agtcaaaaca attgaagcgc gcattcgctt
    19201 ggcaatctct gaccaaagcg gcttattgga aatcgcgctg gcgtcaagct ggtaaacacc
    19261 cctgtctttt ttgtttactt taacacccag gttgactaac aaatcaagca tccgaaacac
    19321 atcctcaatc tccggcacat tgctggtcat aaaaccatcc tggaaaagca cggcagaagc
    19381 caaaatcttg agagcggcat ttttagcgcc gccaaccaca atctcaccgg acaatgtttg
    19441 ctgaccgcct aaaccgtcaa taataaattt gtcagttacc tcaggcacgg tagaaataat
    19501 actagattag agggcaaaaa gcaaaaactt ctaacgcaga cgaaacgctg atttgcgctg
    19561 ataaaaacac agatagatag gttatggggg ttttatctta tcttctgaaa cctgatagtt
    19621 ggaaactatt catccgagtt taacctctga aaatttgcgt cttatctgcc ctagacacgc
    19681 agatccgttg caaacacgcc tttcttcagt tccagcggtt cggattgata aattttttga
    19741 taagaaccgt cagacaattt ttcttccacc gtatagtaat aactccccgg tctgaccaaa
    19801 atataaaaat ttcccacctg gtcagtcacc acattcttaa cctgctgatt aatttccggc
    19861 aaaaacagtc tgatgataga aaaaggtaac ggctcgccgg tttctttttt gtaaagacgg
    19921 cgcggtttat atttagaacg ccacaacttg ttaaaaatat aaaccgccac gtagagcaaa
    19981 gttagaatta tgtttaaaac tgaaggccga aaaaccagag tatagacaga caaaagaaag
    20041 cccaaagaaa aaatcatgtt aaaaatccga tttcgccaga tttctttttt tgaatagaaa
    20101 cggaaaaaat cagttttggt tttagcaaac tcgttccagt caaaaccaat ggggtccatt
    20161 ggaatgttaa ggtttataac ttcctcgcct tcagttgtca aggtttgacc aaaatacaaa
    20221 ttatcataaa gctcatccgc cctttgcccg gccaattttt gcgatggaaa ttgataatga
    20281 gttttgttcg ccttgaccag ataattgccg gcgggcaata aaaagccaaa ccggccatca
    20341 atatccgtaa tagcgctggt tatttctttg ccaccgccga ctggctctat tgaaacgtaa
    20401 gccggatcaa ggggacgttt ggtgaccgaa tcatagaccg tgccccaagg cttagctttt
    20461 ttccgccggc caaataagta gccaaacaaa cgaaccaaat aattaaagat gtcggccaat
    20521 gaggggaaaa aacctaccgc caagccggaa gcaaccaccg ttggcgccgc cgcttcaacc
    20581 gccaaagaca aaagctcggc gcccggacgc tttgttcccg aaaccgagat cttacctctg
    20641 gtcggaacaa ttgtctgatt attataatca atcaaataaa tatacatttc gtaaacgcct
    20701 ccatatttaa aagccggcac ggtcgcgcgg taaacttttt tgtcagccga aacgcgcaag
    20761 ataactgccg ttacgtcttg cggattttcc gtgtccacaa aagttacgcc gattgttttt
    20821 aaaactctgg gtacctgctt gtaattcatt tccaaagtca gagttttacc ccctttgatg
    20881 taaattgttt ccccgcgatt aaagagctta attggctcgc ccggttggag caaccgcaaa
    20941 aaccaagaag cttcactcaa aggagtagag gtagccagag acaaaccaac aaaagggtct
    21001 cttgtttctc tcccgcttct ccccccgtct tccccgtcaa atctccctcc cttgtcttca
    21061 ggctctcctt gttccggctg ttcttgctgt tccttttctt cctctttttc ctcttctttt
    21121 tcttcaactt cttctttctc ctcctctgct tcttctcctc ttaggtcaag agaggcaatc
    21181 gcgccagaag aaaattctcc cttcttgttt ttaacaaaag cggcataata atataagctg
    21241 ccagtcgcaa ccccgctgtc tttggccgat tgacctttgc cttggaaaac aagaatcccc
    21301 tcacttggat tattgggaat ttcccccttc aaacgaacta tcctaacaag cgaaaaatct
    21361 tttttggggt tacgccaaga caaaaaagct tcgtttttgt tttctctgta aattaccgaa
    21421 aaatttgtaa catttggcac gcttggatat tggcagtaac catcatcaac cgtggcagtt
    21481 gagtcataat ttaaagcttc ttcgtcagtg caaccataaa catctttggg cggcgtcggt
    21541 ccgccaccat aagtacagct accgtcgtca attgtggcgc ttgaactata attgctagca
    21601 gaaggatcgg tacaaccgct gacctcagaa ccggagataa cttccaaacc aacttgaact
    21661 gagtccgtct ctgccccaag cacaaaagga gttacaaaca aaaataaaag caaaaacaga
    21721 ataaaatttt ttttgaaatt aaacacttta ttggcttaaa gcagttaagg taatgtccgc
    21781 cgaataagag cccgctgttt gggtttttcc gctgccgatt tcggccttga attttacagt
    21841 tgtaaccgtc ccggctggat tgttggcatc attattagag gcaattagat aatcagaggt
    21901 tgataagtta taccaacaac gatcagcggt aatttcagaa ccaacattgc aagctgaacc
    21961 attattttta aaagcggccg ccacatcact gccagaaaca gtgaagccaa actcagaatc
    22021 agttgagtca accgaccaga caaaatcagg cacattaact gaagctggcg tataatcagc
    22081 gaaactgtca cccgagcttt gcaacgctgg cgcagcgcca gcgctaatat acaacaagta
    22141 gccactcgga tcgttcgttg ttacagtcca gcttccactg ccatctgatt gcccgccggt
    22201 cgtcgtcaat ccagccaaag agatattctc tggcgccgaa attgtaattg tgtaatcccc
    22261 ggccgccgcg cgataaccgg ctttcaccgc gtaaagaatc ccagatgaat ctcccgacgc
    22321 aaactctcca agagtgtcac tcaagctata atttgaagaa gtcgaattct ccgtcccacc
    22381 aaaattaaag ctgtcctttt ccatccgata gttggaactc tcagacacat aaccgaaaac
    22441 caagcccgag ctggcaaaaa atatcgtaaa cgcggccaca atttttttaa ggttaaacat
    22501 tttttttgcg gattatttta aagattatca cccaaacgaa gaacaaaaaa acagcggaaa
    22561 aaattaacaa aatcggccaa ggtaaaaacc aaaaactggt ttccctcata tcaacttcgt
    22621 cattataccc tcggttaagc aacaagaagg cggaatagag gccggggaaa cccaacgcgc
    22681 ttgttttggt ttcaaataag cgagtttgac cgggcaaaac tgagcgtgga ggaatatcca
    22741 ctaaagtttg agaacccgtt aatctgttga ccaaaatcat tttaccataa ggattcaaat
    22801 aaatattgcc agaattttca aaagaaaagt gccactccaa aggagctgca aattttaatt
    22861 gtcgccccaa aattccaaaa gaaaccagtt tcccctcttc tttaactggt cccccaacac
    22921 gaacaaaaat agttgagccc agggcggctg aaacgcccgc ttctcctttg gtcccctgat
    22981 tcggactggc gaccacttgc aggaaaccat aagctcctcc cggggccgtt tccgtcggaa
    23041 tattaattcc aaaataaact gtttttgttt gaccgtcagc cacaaaaact ctttcatctt
    23101 ccaaatttac aaaatcagtt aacggataaa ctcccagtcg atttaattca atatttttta
    23161 acttaagctc aaaataagaa ttgcgcccaa ggttgttagt aattgtaaaa gattcttgcg
    23221 ctgaggcacc ggggttaagc acaacttctt gtttggctgg cctgagcgaa aaagattcag
    23281 ccagcgccca acaagggaaa agaacaacgg ccaccaaaag aaccggccga caaaaatttt
    23341 tgattcctaa cttcatcatt gattaattgg cgtcaataaa aagacataat cgtcgccttt
    23401 ttcgccatct tcaaatatcg gagtgatgac cccggcttga ccacttttat caaaactgat
    23461 agaaacctca tcggaaaact gtttctccag gtcttttttt atcttttcgt cggcggacgg
    23521 atcgccggta gatggcacga ctaccgctcc gtaggaagcc ctcattgtgt catcagcgac
    23581 ttgaccactt tgactttgta attgtaagtt tttggaaatt tcttcggcga ccactttttt
    23641 aatttccgcc atggcctccg gactaacttc gcttgggttt ataaaattgc gccaattcaa
    23701 cgcaacttcg tcggccttaa gcaaagttgc tgtccaaata gcggaaattt tttgatttag
    23761 gcgaaccggt aaattttcaa accaaagtaa accgttatca agggtgatag cccatttgat
    23821 aaatctgatt gaccatctct catagatttg gcgaagagtg ttttccattc tgcccaaatt
    23881 aatcatagaa gccgagttcc aggttgccgc cacttgcggg ggcagagtct tgacttcttt
    23941 gcttacctga gacgcccccc gcttaacgga agcaaagaac ccagagtcaa caccgaagac
    24001 tttatccaaa acaactagag cgcgcgcgcc aagtaactga cgacgaatag cgactttttc
    24061 tttaactcca tttatgttca ttgcccaact gttgctcttt tgaatcaaga ctaagctgga
    24121 gtcccgactg gcttgttcta atttgccgac taattgattt tcgccaatag ccaaagtttt
    24181 ggtaacctgg ctataccccc tcttgaccaa ttcaccccaa ttggctgtca cagctgccaa
    24241 atttttttgt tcaactcctt ttgttaaata agcgaccttg tccaaagtat aatccagagg
    24301 cttggttatg taagcataag ttaccccaat agtgtttgta gtcattgcca ctatttttgt
    24361 cccggctctg acagccgaat cccaatagag caagtccgtg ctcaacaagg ataaaacaaa
    24421 cagggcgcca agcaacactg ccggccacgg gttaggtctt tgagccggta aaatgtccgc
    24481 tttaacagct ggcgacaaag cgcgacgacg ccaaaacagc tgataaccca accagttctt
    24541 tttggttttc gacaaaagag ttgtctgttt atcagagaca aggcttctcg tcaaactacc
    24601 tttgtcaagg cttgattgat tttcaggttc agtcatcttt tattgcctaa attataacac
    24661 ttttgacaaa ccggcgacaa ctggcaccgg ttgtgaataa ccccaaaaga ctatatactc
    24721 taaaatgaag gcctatgaaa atagttgaga ccattcctat tgcaaaaggc gtttttaaag
    24781 agcgtctagc ctatttttcc aaccaagaaa tcaagcccgg tatgctggtg gcagcgccct
    24841 taaaaaacaa gactataacg gccctagtgg cttcagttaa agaagcagga gaccttaagg
    24901 cgcaaataaa aaattcggat ttcgcgatga aaaaactggg ccctatcaaa aacaagggtt
    24961 tctttctgcc cgagtttttg gcaagcgcca cttggtcggc agagtatttt gcctgctcgc
    25021 tcggtcaaat cataaaagag ttgacaccga aaattatttt agataattcg gaaaacatca
    25081 aagttacgct gcctaaagaa gacgccgagg atgttgtttc agaaatccaa gcttcacagg
    25141 cttcggagga agaacgttgg tctttttaca aaagtcttat tcgagaagct tttgccaaaa
    25201 agcaatctgt cttctggtgt ttgcctaatg tcaatgaaat aaaaagcgcg caaaatttta
    25261 tcgctcgtgg catagaacaa tatgtgataa ttttgcacaa caaactatcc aagaaagagt
    25321 tgttgaccgc ctggaaaaaa gcgattgaaa atgaccattc tgttttgata attgccacgc
    25381 caatgttctt gtccttgccg cgcgaaaaca taaaaacttt tatcatagat aaagaaaatt
    25441 cttccgctta taaaagccgc gtccgaccct tcttagattt tcgtcgcttt gccgaggatt
    25501 atgcccgacg ccgaaaagtc aggttaattt ttggtgatat tgttttgcga agcgaaacga
    25561 tttatcgtct tggccaaaac gaaatcacgg caatcaaccc ggttaaatat cgtctttacg
    25621 ctgaaatcaa gcaggtcatt gccaaactga acaaaaatag tgaagaccga gtcaccgttg
    25681 gcagcctaag tcaagaactt ggttggttaa tagaaaataa caaagaaagg agcgaaaact
    25741 tgtttatttt ttccggccga cgcggtctgg cgcctgttgt tatctgtcgc gactgtcaaa
    25801 ccatggttac ttgtgatgtc tgccaaacac cattggtgat acataaaaaa acaccagagc
    25861 gccagactgg ccgaatgttt atctgtcacc gctgtaacca ggccgttgag attgatgacg
    25921 cttgtgaggt ttgcggcggg tcacggctgg cggttcttgg ttatgggata gaaaaaatag
    25981 aagaagatat cagaactctg ctacctgaga taaatatttt tcgtctggat agcgattctg
    26041 ttaaaaacaa caaaaaagcg attgaggttg tccaaaaatt tatgtcttcg ccagggtccg
    26101 ttcttttggg tacggaaatg gccctgcctt acttgcctaa cgagattgag aatgtcgcga
    26161 taggtggaat agatgctttt tttgccttgc cggatttccg aattgctgaa aaactttttt
    26221 ctctgctttt tagactccgc gccaaagcga ccaggcgttt cttaatccaa accggcaacc
    26281 ccgaggaaaa agtttttgat tatgttttgg gaggcaactt gttggacttc ttccgtgaag
    26341 aaatcgctga gcgtaaaaag cttggttatc cgccgttcaa ggttttgctt aaagttagtc
    26401 tgaccggccg acccgaaacg gcgaagcgcg agatgaaaaa actggctgac aaactggaaa
    26461 aatacaaacc actcgtttac cctaccttaa caggcgccat taaggagtat gagcaaaacc
    26521 ttttgcttaa gctggaacca aaagactggc caaacccaaa gttatcagaa attataaaaa
    26581 acctccctcc ttcttttgtt gtggaggttg accctgaaaa aatactctga tatgggatgc
    26641 ggatagcaca tagatctcgc gcagcccaga cacaaaatta atgcggctaa aaacgcgctt
    26701 gccgccttgg gcagagattt atgacaattt ttcattttac cttctaaccc taaatcccct
    26761 aacctcttcc actaaaagat tggcttgctt cattctatat tccgtataat atatactaca
    26821 tacaatacac aattcgacga tatacaacac acgaatatga aaattgtcca aaaagacgac
    26881 ccggtcttgc gccaaaaagc caaagagatt cccttggaaa aaatcggttc accggaaatc
    26941 aagaaaatca taaaacaaat gagtgaggcc ctcgtcaaag aaaatgacgg ggttgctttg
    27001 gccgcgccac aaatcggcaa aagtctccgc ctttttatta tttccgggaa gctttttcaa
    27061 aaagaaaatg acaaaagaaa accggccgat ttggttttta tcaatcccaa aatcagaaaa
    27121 ttatcccagc gaaaacaaaa attggaagaa ggttgtctct ccgtgcgttg gctctatggt
    27181 ttggtcagtc gggccgacaa agcaaccgtc gaagcctaca atgagaccgg ccaaaaattc
    27241 agccgctccg gttcaggctt gatggctcaa atttttcaac atgagatgga tcatttggac
    27301 ggcaccctct ttgtggacaa ggccaaaaac ctacagcgca tagagccgga gaaaatgaga
    27361 cacggaaaca gaatataaat taaagtgtaa aaattaaaaa ctaaaatctc tttcctttca
    27421 ttttaatttt tacattttgt acttacctaa taactatgga caaaactttt attttttggg
    27481 gtacagatga atttagtgtc aaagttctgg aaactctagc gaagcgtgaa ttagaaccag
    27541 ctctaattgt cacggttccc gacaaaccgc aaggacgcaa aaatatcatc gttccctcgc
    27601 ctgccaaagt ttgggctcaa acaaaaggaa ttgatgtcct tcaaccatct tcgctacgta
    27661 acccagattt tgtcgagcag ttgaaggtca agagagtggt attttttctg gttgcttctt
    27721 acggcaaaat aattccgacc gacattttgg ccatcccgcc caagggaacc ttgaatattc
    27781 atccgtcact tttgcctaag tatcgcggtc cctctccgct ggaaaccgcc atcctggctg
    27841 gcgaaaccga aacaggcgta acgattatgc ttgtggatga agaaatggac catggtccaa
    27901 ttctcgcgaa tcaaaaaatc agcctgcgag atcaatacag ttttgagcga ctaagagacg
    27961 aaacggccgt gattggcgcc aacttgttcg ccgatatttt gcctggttat ttagccggtt
    28021 ccttggaacc caaagcgcaa gagcactcgg ccgccaccta caccaaaaaa tttaccagaa
    28081 aagacggtcg gcttgacttg tccgccaccc ccacggaaaa ttatcgaaag attctcgcct
    28141 taaatccctg gccaggcgcc tatttcattg accaacatgg taaccgtcaa attaaagtta
    28201 aagtcaaaaa agctaaacta gaaaacggag aactgatctt tgagcgtgtc atccccgaag
    28261 ggcgcaaaga agctagctgg gctgattatc tccgaggcaa acgttaaagc gcgcttgcca
    28321 attataacca gcgttattgc caatcttaac tcggcaaacc tttttctttg cgacccgggt
    28381 aaaaaccttt gataaagttt tttaccgctc gcccgccacg acgcaagaga tgatttttct
    28441 ttttcagata agaagagatg tcgcgttcaa ttttgtcttt ggcttggtcg atcgccgcgt
    28501 aaaggtcgtt gtcattgaca acttggcgaa aaaacttgcc gtcaatcgtc aagtttatct
    28561 cagcaaacat tatttcgccg tttttgtgat gattggtaat tttaccaact tccacttccg
    28621 ctaacgaatt ctcgtaacca accattagct tgtccaaata agaaattttg gactcaatat
    28681 aatcggcaat cgccggagtc atttccaaag aggtagcctt gatgttagtc ttcatatttt
    28741 tatgaattaa acttttaatc ttggccaata attattttta ttatagcaaa attcaacccg
    28801 aatttaaagc tcaagtctga cttctgattt ccagaatttt ggccggatta tgatggccgt
    28861 gaatgattct gatttgtccc acggccaccc ccaaatgttc cgccaccagt tcaatcactc
    28921 gccgattagc ctggttgcgt tccggtttgg cgcgcaccga aacaatcaag gtgtccggcg
    28981 ccaattccgc cacttcatct tttttagttt caactgaaac acgaactttg atgtgcacaa
    29041 gagattttat ttaatttcgt aacgcaaagt aacaatctca acaatatctt gcgagccggg
    29101 ttcaatagta ggaattgcgt ccgacgccgc tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29221 nnnnnnnnnn nnngtcgttt caatgtcttt tttctcaatc gccaaagttt tcaggtaatc
    29281 aatcactttg ttgaccttct tggtattctc ggcctgaatt tcgcttaacc ctttgccgcc
    29341 ttcggaaatt actgttgccg taaaagttgc cacatccgga atagctgtca ccttgccttc
    29401 cccagtcacc gaaaaactct tataagaacc cggctccacc gctttggcgt aagaccaagc
    29461 atagctgatt gctgaataag aaacggctgc catcgccacc aaagccatta tcgctaacgc
    29521 attttttaat ttttgttcca tatttttaat ggttaaattc ttataataaa actaattata
    29581 ccactgaatc acactaacga aaagacggct aaactctgtt atttcacgag gaattattat
    29641 aaaatgaaaa tattaccctt caacaaaaaa ataatgtttg tcataaaaaa aatcctggct
    29701 agagaaattt tagactcccg cggcaaccca acggtggaag ccgacttgtg gttggctgac
    29761 ggctcatttg gtcgcgccgc tgtccctgcc ggcgcctcta ctggacaata tgaagccaaa
    29821 gaattaagag atggtgatca tggccgcttt ggcggcttgg gcgtcttacg ggctgtcaaa
    29881 aatatcaatg aagaaattat gtccaagttg ggcaaagcgg aattaaccca aaaaagttta
    29941 gatgagcaaa tgattgagct agacgccacc accgacaaaa gccggctggg cgccaacgcc
    30001 atcttggcgg tgtcgctggc ctttgccaag gccgcggccc aaagcaagcg tcaggaactt
    30061 tatgaatact ttgccgaact gtccgacacc aaacaaataa cgatgccggt gccaatgatg
    30121 aacatcctaa acggcggtcg acacgccgaa ggggcaattg attttcagga atttatgatt
    30181 gtgccaatag gggcgtctga ttttaatgaa gctttacgtt ttggcgcgga aacttttcac
    30241 gccttgaaaa aaattctctc ggcccgaggt ttatccacca cggttggcga cgaaggcggc
    30301 ttcgcgccca a
//
LOCUS       DAOOLO010000003        27060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_1602_length_27060_cov_6.878578, whole genome shotgun sequence.
ACCESSION   DAOOLO010000003 DAOOLO010000000
VERSION     DAOOLO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 27060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27060
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_27060_cov_6.878578"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLH96_00350"
     CDS             complement(<1..539)
                     /locus_tag="PLH96_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPB60261.1"
                     /translation="MSKRILTLVVAAAFIFALVGVSTAKAATVEELTALIAQLQAQIA
                     ALTGGSTTAAGEITKDLTIGSRGDQVEILQKYLEDEGYLVMPVGVAYGYFGNLTKAAV
                     AKWQADNGVAPAAGYFGSISRAKYASLNPVTPVTADDDDDADDADLEGGAGAISDADY
                     IAKLSNEEVGEGESDVEVAG"
     gene            complement(1190..1996)
                     /locus_tag="PLH96_00355"
     CDS             complement(1190..1996)
                     /locus_tag="PLH96_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60262.1"
                     /translation="MGQGIDSRFLKFAFLKSKKLVQATYLVTSFISDSEPLKWRIRES
                     VLELMSDTSLLVPTSSLSDGSGEGQISPLFKISVLEAVLNRLDQVANLLDIASAAGFA
                     SEMNLAILKNEFRGLGKNISSKLGRGLKEIVGPEEASQLLLEAREKYPVGGVATTMEH
                     NISETNYNANVRLSEMLSERLKGTEAGKEKDLYQKEGGKKNRRLVNKDARRLEIISFL
                     KGKSWTSIKDISDAISDCSTKTIQRELADLVQKGVLKKKGDRRWSRYLLA"
     gene            2308..4557
                     /locus_tag="PLH96_00360"
     CDS             2308..4557
                     /locus_tag="PLH96_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPB60263.1"
                     /translation="MGRHKKNRNGKEKKSRRDDEGLPSLHLKNETKKAVLAIILFALS
                     LFFVLSCFNIAGLAGVVTYNLFYKLFGHGAFLLPVLLVLLGIYLLRSLEFKLSPSRVI
                     GGLIFFLSSLSIIQVIAGDTSGGVVGGFLASPLLRYFDFYASLIFLSALVLISLIITF
                     DSHQVLGFSLWQKLFKKKEEEEEQEEKFETIKLSDASKEALREEYPTAETDKADKEPN
                     KEKELKVNDGGFLSGAPLFGKKAKTTGAFIPPPISLLEQDSGKPNAGDIKANANIIKR
                     TLSNFGISVEMDEVSIGPTVTRYALKPAEGMKLSRIVALQNDLALSLAAHPLRIEAPI
                     PGKSLVGIEIPNKAKATIRLASLLSSNDFAESHHPLYVALGRDISGQPRFANLAKAPH
                     LLIAGATGSGKSVTIHAVISSLLYRNSPEKMRFVMIDPKRVELTLYNKIPHLLTPVIT
                     DSKKAIAALRWLAKEMDRRYDILEAEAVRDIQSYHKNVLEPTLAKAATKKTTAEEGEA
                     DEADEVEQMPYIVVIIDELADIMTAYPRELEAAIVRLAQMSRAVGIHLVLSTQRPSVE
                     VITGLIKANIPSRIALQVASQIDSRTILDMAGAEKLLGAGDMLYLSGEMSKPVRLQSA
                     FISEDEVKRISAHLAKQYADIPPSDLNLGAEGNSGGSPLFNATFGDSDADDDLYEDAR
                     ESVIQAGKASASYLQRKLKVGYARAARLLDMLEERGVIGPGEGAKPREVYGAPSAPEI
                     LEGDDENMI"
     gene            4572..4940
                     /locus_tag="PLH96_00365"
     CDS             4572..4940
                     /locus_tag="PLH96_00365"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60264.1"
                     /translation="MTIETKGLKERISAKAIITILVVAVILTYSGFKFKDLISGPDIV
                     LYSPSDGSSLNNELVNIKGRADRITQIYLNGRKIFTDQDGNFEEPLLLSSGYNAFELK
                     AEDKFGREIVRKLQVVYNKS"
     gene            5038..6111
                     /gene="recA"
                     /locus_tag="PLH96_00370"
     CDS             5038..6111
                     /gene="recA"
                     /locus_tag="PLH96_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPB60265.1"
                     /translation="MAKKTKQTKTTGNNIADTIKAIQSKFGDEAIMKLGDKPKVGIDA
                     VPTGSLALDAALGVGGVPRGRIIEIFGPESSGKTTLALHIVAQAQKKGGVCAFIDAEH
                     ALDPEYSKRLGVQTEELLISQPDYGEQALDIAESLVRSGKIDVVVIDSVAALTPKDEI
                     EGDVGDYHVGKQARLMSQALRKMTSIVDRSKTVVIFINQIRMQIGVMFGNPETTPGGK
                     ALKFYSSVRLDVRRIAQIKKGDEVIGGRIRVKVVKNKVAAPFRQAEFDLLYREGISPE
                     GELLAIGEKYNLIKKSGAAYSYTPDGKTTHEVKLGRGYDAARQFLRENSDLKQEILQK
                     VKEKLLNNDEDSSNEETDTNDEE"
     gene            6180..6740
                     /locus_tag="PLH96_00375"
     CDS             6180..6740
                     /locus_tag="PLH96_00375"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB60266.1"
                     /translation="MLEYNEIKPRKFIVFEGEPFEVLTSHVFRKQQRKPVNAVKMRNL
                     ITGKVIENSFHQSEKVEEAELERRPIKYLYENRGQYWFCEVNDPSKRFEMPAETIADK
                     IKFVRQNDTIDVLSFGDKVLGIEVPIKVELKVTDAPPAIKGNTVQGGLKQITLETGFT
                     TNAPMFINEGDTVRINTETGEYVERV"
     gene            complement(6864..7067)
                     /gene="rpsR"
                     /locus_tag="PLH96_00380"
     CDS             complement(6864..7067)
                     /gene="rpsR"
                     /locus_tag="PLH96_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPB60267.1"
                     /translation="MNKQCHFCTGKSKVVDYKDVEILKSFLDTHGRINKHRRTGTCAK
                     HQRQVATAIKQARFLALLPFIEG"
     gene            complement(7188..7649)
                     /gene="ssb"
                     /locus_tag="PLH96_00385"
     CDS             complement(7188..7649)
                     /gene="ssb"
                     /locus_tag="PLH96_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPB60268.1"
                     /translation="MYLNKAFVIGNLTRDPERRALPSGTTVVNFAVATTLYWKDKNGV
                     RQEDTQFHNCVAFGAQAETIAQYMRKGSSIMIEGRMQTRSWDAQDGSKKYRTEIIVDS
                     FQFGPKREGGSSFDTGSPAPAGKPESASVPPSELETIQYPEEEINPDDIPF"
     gene            complement(7688..8224)
                     /locus_tag="PLH96_00390"
     CDS             complement(7688..8224)
                     /locus_tag="PLH96_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB60269.1"
                     /translation="MDEIEKAENLDNGEEQEPKIYEIGYLLTPIIPQDALAENVVQTL
                     KAPIDKLGGVITSEMDPSMIRLAYTIKKDIGNKKERFNDAYFGALRFKLLPEKAAVLK
                     DVLDKSDKIIRCLTIAMPEGEETIVLPKRTFQRREVQTEPFEIERREKEVEPEEVKEM
                     TKEDIDKEIENLLEEQAS"
     gene            complement(8358..9176)
                     /locus_tag="PLH96_00395"
     CDS             complement(8358..9176)
                     /locus_tag="PLH96_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPB60270.1"
                     /translation="MPYSTPNTYNLKPVLYFDAHTHLNLQFEKDWQAVGERAFEEGVS
                     FVNIGADLASSQKALEQAEFFGGGGFCSVGLHPTDGRLADFGEITALAENPKVVAIGE
                     CGLDYFHCAEESERQAQVELFKKHLDLAFVLNKPLMIHCRPSVGTFDAYEDLFQILRE
                     YQDFGKLPRFNMHFFVGDWPLAQKFLALGGYLSFPGVITFSEQYGEIIKKMPLNRLMS
                     ETDAPFATPVPFRGQRNEPAYVKYVAEKIAELRPEMSAVVLAALVQNAQEFFSL"
     gene            complement(9348..10817)
                     /gene="metG"
                     /locus_tag="PLH96_00400"
     CDS             complement(9348..10817)
                     /gene="metG"
                     /locus_tag="PLH96_00400"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPB60271.1"
                     /translation="MSSKFYITTTLPYVNAAPHIGFATEIVRADIFARWRRLLGDEVF
                     FNTGTDEHGQKIYQKALAEGKTPQAYVDEWAKKFKGLQSLLNLSPELNFIRTTDEHHI
                     SAVQEFWRRCRANDDIYKKFYKIKYCVGCELEKQESELEDGKCPLHPTLELQIVEEEN
                     YFFRFSKYQKSLLELYEQKPAFVLPKSRLNEIKNFVSAGLQDFSVSRLVGKMPWGIPV
                     PDDPAHVMYVWFDALVNYLSAIGWPDDEARFSRWWLESGGAVQFCGKDNLRQQSAMWQ
                     AMLLSVGLPPSKVILVEGFIISGGQKMSKSLGNVVSPLEIVAEYGVDAFRYQVAREFS
                     IFEDSDFTWERFKEAYNANLANGLGNLTARIMKMSENYFAEPIATEDTFESVITTVGN
                     ISSVWEQYNDALGAYDLNRAANVIWREIGEMDREIQERAPFKLFKTAPEEARKIVRTL
                     VFRLARLAVMLSPFLPETAEKIKQAILVNKMPEPLFVRK"
     gene            complement(10807..11856)
                     /locus_tag="PLH96_00405"
     CDS             complement(10807..11856)
                     /locus_tag="PLH96_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB60272.1"
                     /translation="MLNKEINFNINISVVLKIAVVVTVIILSYFLRDIFLALLTAVVI
                     ASAVDPAATRLSRFGLPRVVGVLLVYLLSFLILFSVLYLFVPPVFQDITGLAYTLPKQ
                     INTFIGNNPAWNSIVSFSDSLDTEFSIQEIISRGLLDSPIPGNVYDLVKSLFNGVVSF
                     FLIIVISFYFAVQKNGVENFLRVITPLKQEEYVINLWQRTEVKIGRWIQGQLLLGLIV
                     GPMVYIGLTLFGIKYALVLAITAGLFELIPVFGPVLAAVPAVFLGFSENFTLGLMVIG
                     FFIIVQQFENHLLYPLVMKKVIGLNPIIVIISLVVGYELAGFLGMILAVPATTLLMEY
                     VNDVGKRKKGLSNVV"
     gene            complement(11864..13132)
                     /locus_tag="PLH96_00410"
     CDS             complement(11864..13132)
                     /locus_tag="PLH96_00410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB60273.1"
                     /translation="MTPKKTILTNGIRVILLPRPDALSVSFLILTNAGAKNELDQEAG
                     LAHFIEHMGFKGTPKRPNAKIIASEFDSLGATYNAFTGYDFTSYYVTAVPDKTVQVID
                     LLADIYLNPIYDEAEMEKEKGVVMEEFKSYEDKPASFVWDVFLGATYGDTPQGRTTLG
                     KKETVAGFKRSDLMNFRRRHYLTDSTVLVVSGRFEEEKVKAQLEKLFVPLPSGRDVRP
                     LFKELAQSQPKVAIATRPIEQSHIVMGFRGFDIYDPRYFRLSVLSNILGGSTSSRLWQ
                     RVREELGAAYYVGTSGSNRTNFGYFALYAGTDSRKDKQIVGVMAEECRRLKNSLVTDE
                     ELIRAKNHLIGNLYLGLETSFDLGYFFGGQEIYRREILSPGEFAAKINAVSADDVRSL
                     AQDLFKPTSLNLAAVGPNQSKDDYLRELVI"
     gene            complement(13210..14604)
                     /locus_tag="PLH96_00415"
     CDS             complement(13210..14604)
                     /locus_tag="PLH96_00415"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPB60274.1"
                     /translation="MGEKENLLDKVVSLAKRRGFVYPSSEIYGGFAGVYDFGPMGVEL
                     ENNLKTHWWKWMVHKRQDIVGLDSSIFMNPKIWEASGHTKGFSDPLAECKECHTRIRV
                     DKVLGDIGVFADEKMSEEEINKLFNEHRQEIKCPNCGKQNFTDARRFNLLVKSNLGDF
                     TESGLDNPSYLRGETCQGIYVNFKNVLDSNHKKLPFGIAQIGKAFRNEIAPRQFLFRT
                     REFEQMEMQYFTHPNQEMAEYEKLRAYRWQAVLDLGLREENLRWKRHENLVFYAKEAY
                     DIEYNYPWGFDEMEGVHARGDYDLTQHAKGSGKDLFYQDTESGEKFIPHIIESSGGVG
                     RLLLAVLCDAYTEDEMGGESRVFLRLKANLAPVKVAVFPLLKNKPELVAKAKEIFEKL
                     NEKIQPIVFDDNGNIGKRYRRQDEIGTPYCLTVDFDTLTDAAVTVRDRDTGVQERVKE
                     TELLSYLSTKGLAF"
     gene            complement(14619..16553)
                     /locus_tag="PLH96_00420"
     CDS             complement(14619..16553)
                     /locus_tag="PLH96_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60275.1"
                     /translation="MEQAPKSSGFTSRLEAMQKKIYRDKPDEEMLESESTLLPKKKFG
                     VKTDWDIPAEETKSEKKPLTSSFLFRTFLFSVVFFILALGVAAYLFFYGFNTISSHNI
                     EMKIQGPSIVKAGDEINLQATITNNNKAPLDSVSVIFSYPVGTLNALDRASAFPVDEQ
                     EIGGISEKQVVNVSSRAIIFGERNSEQEVVVTMEYRLPDSNAIYTKEKTFTLVMGASP
                     IDLSLDLPSELNAGQEFPLVIKLVSNSETVLKNINLTLEYPATGFMLKNAEPEPLAGG
                     SVWFLGDIPAGSERVIKINGILEGQNEDVKSFRVRVSSSPLGSPDEDSFDYGEAFAKA
                     VIKRSFINFGLSINGSTRSEVVIRPRDEVSVSLNWGNNLPDRLFNNRMVVKLDENLID
                     KRSVVSNEGFYNSIDNTITWDRTNTPGLSELDTNEIGNSFFKFKLLDFSQEAVFSQPS
                     FGISAFFEGQRVGADQLSEKVTSDSQRLVKIETEADFSARSIHSVGPFKNSGPVPPRV
                     GQDTSYTIVWSITNTTNTLNQTKVKTTLPAYVNWTGSISPPEEKIIYDPNTREVSWQV
                     GEVSAGTGETAPVREAYFQVSITPSLSQVGQLVRLTNQSHFQATDSFTNTIMTKTGEA
                     VTTDLTNDPKFGRNDQLVTE"
     gene            complement(16583..17185)
                     /locus_tag="PLH96_00425"
     CDS             complement(16583..17185)
                     /locus_tag="PLH96_00425"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HPB60276.1"
                     /translation="MSYKIYHSEGFILSGRPYGEANKVFRILTPDLGLVNCLAQGIRL
                     EKSKLRFGASVRRFVKVSLVRGREYWRLIHVELDNNFNNLVTDCAPDGLEMFLAKTAT
                     LLSRLIYGESRDPDLYEILKGAVTAWVKNSNDGKELQQSLEILTVVRLLTVLGYRSDD
                     KGIGLFAEAGDWQKNTLVDFKTHLPRAVRSINDVLQNSHL"
     gene            complement(17189..20752)
                     /locus_tag="PLH96_00430"
     CDS             complement(17189..20752)
                     /locus_tag="PLH96_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB60277.1"
                     /translation="MLTENEESQKSDVAKREEEVLAFWRENQIFEKSLTKDSPKGEFV
                     FYDGPPFATGLPHYGHLLAGTMKDVIPRFKTMKGFHVRRRWGWDCHGLPVENLVEKEL
                     GLKNKKDIEDFGIDGFNEKARESVLRYADEWKRIVPRMGRFVDMEDDYRTMDSTYTET
                     VWWVFKTLYDRGLIYEGYKSMHICPRCETTLSNFEVTQGYKDVTDISATVKFELVDEP
                     GIYLLAWTTTPWTLPGNVALAVSKEIVYCQVKSQRPKAKDEAQNRGEGEGVLIVAKDR
                     LAEVFKDAEYKLEKEFLGADLIGKKYKPVFDNYKDLPLKDVKGKDIAGVAKVWQIVEA
                     DFVTTESGTGIVHIAPAFGEDDYQLALKEGLPFIQHVNMSGEFKSEMGELAGQKVKPI
                     ENSQATDIEIIKLLATRNLLFSKEKITHAYPHCWRCATPLLNYAASSWFVKVTDLKEK
                     LLEANQGIGWVPAHFREGRFGKWLSGARDWAISRSRFWGAPLPVWRCEVCNQTEVVGS
                     LADLIKLQQDRNNTFTFMRHGQAESNLRDAISIDPNGPNRLTEQGKEEVRLSAQKLKE
                     KKMDMIVSSDFVRTKETAELLAEELGLSEDEVVFDERLRELNTGNFLGKTWAEYDSQV
                     GPLRKRFERQPEKGGENYNMVRRRMMALVYELNEKYQNKNFLIVSHGLPLFLATATMR
                     HLSLAEMEKEKGFATGEVREVPFLSVPHNKNFELDFHRPYIDDFALSCACGGNLRRIP
                     DVFDCWFESGAMPYGQDHYPFETSRIEPKTGKGFPADFIAEGQDQTRGWFYSLLVLGI
                     GLFGESPYRQVIANGMVLAEDGQKMSKKLKNYPDPMVVVDRYGADAVRFYLVSSPAVR
                     AEDLNFSEKGIAEIYRKNIARLLNVLSFFETYTEELPTKEFNSRQLVNVLDRWIMSRL
                     GGTIVEVERVLDNYEIDRASRPIEEFIEDLSVWYLRRSRDRFKSDDKGDREEAIATTY
                     HVLKETAKLLAPICPFVAEEIYLKLKKASESESVHLAKWPNEENNLVYIETPIGEEMA
                     AVRQIVSLALEFRQAAGLKVRQPLRELRVKSQYLALRPEYHNLILEELNVKKLVFDPK
                     LSDEVWLDTEMDDGLKQEGESREFIRNLQEYRKSLGLTPNDKIVLCVEAPDEGREMLR
                     RFEKEVIKAAGLKRLDYGIVKEGWEILVNGKTFKVKIGEDD"
     gene            complement(20913..22601)
                     /gene="argS"
                     /locus_tag="PLH96_00435"
     CDS             complement(20913..22601)
                     /gene="argS"
                     /locus_tag="PLH96_00435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPB60278.1"
                     /translation="MIRKELKREIEKALMALEVGFPGLDTNLEQPADFKFGDYSTNVA
                     MVVAKTAGLNPKDLAEKIAEQIRLNNNPNIKDVQVAGGGFVNFYLSEKFFAESLRAIL
                     KTGDNYGRNQTLSGRKVVVEYTDPNPFKEFHIGHLMSNSIGEALSRIIEANGAETKRA
                     CYQGDVGLHVAKTIWGYFQAEKKENLDIHDWGKFYVAGSKAYETDEETKKEINDINRQ
                     VYGRTNDKTNQVYDLGRQASLEYFAEIYKRLGTSFDFYFFESETEKIGKKMVEVGLSK
                     GIFEKSDGAIVFRGEKFDESMHTRVFVNSLGLSTYEAKDLGLAKLKFERYPYDLSVLV
                     TGNEVLDYFRVMLKALDLLEPELAAKARHVAHGMLRLPTGKMSSRTGEVITAVSLIEE
                     VKKRVLEKTSERELPVEKKEEIAEKVAIGAIKFSILKQSPGRDIVFDFDRSLSLEGDS
                     GPYLQYAYTRAKSVLAKADKEIVDFTQGEEPMVIEKLLIKLPAVVERSFQELAPQHIV
                     TYLVNLASSFNSYYAKNKIIGSENEAYRLVLTRAVAQTLKSGLTLLGIPVLEEM"
     gene            complement(22653..23075)
                     /gene="dut"
                     /locus_tag="PLH96_00440"
     CDS             complement(22653..23075)
                     /gene="dut"
                     /locus_tag="PLH96_00440"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018023885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPB60279.1"
                     /translation="MIIKIKKLNPEAKLPIYAHPGDAGMDFFALAETEIKPGEVVRVK
                     TGIALELPVGYVGLIWDKSGLAANHGLKTMAGVLDAGYRGELQIVVTNLSKEIYSIKK
                     HDKVAQMLIQRIESPELIEVDELSDTSRGEGGFGSTGK"
     gene            complement(23141..24319)
                     /gene="glyA"
                     /locus_tag="PLH96_00445"
     CDS             complement(23141..24319)
                     /gene="glyA"
                     /locus_tag="PLH96_00445"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB60280.1"
                     /translation="MKDKQIEKLLKAEAGRQKKVVNLIASENYVSKDVLTALGSVLVN
                     KYAEGYPGKRYYGGNQITDQVEELCRSRALKLFKLSSKKWAVNVQPLSGSPANLAVYL
                     ALVPQGGKIMGMALDQGGHLTHGHKVSVTGKVWQQVPYGVDSKTEQLDYEAIKKLAQK
                     EKPNIIVAGFTAYPRIIDFKKFREIADGCGALLMVDMAHIAGLVAAGVHPSPFLYADI
                     VTTTTHKTLRGPRAGLIFSRIDERELDKKIDRAAFPGLQGGPHLNQIAATAVALAEAM
                     KPEFKKYAKQVVKNAKVLAKELQKKGWRIVSGGTDNHLLLVDVVASTGGRLAGKEASL
                     LLEKEGIIVNKNTIPFDARSPFDPSGLRLGTAPETTRGKTEKDMVKIAEKIDKILSRH
                     "
     gene            complement(24339..24911)
                     /locus_tag="PLH96_00450"
     CDS             complement(24339..24911)
                     /locus_tag="PLH96_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB60281.1"
                     /translation="MRLAVIGPQNTGKSTFIKDFIKAFPNYKTPQKTYRDVISKRKLS
                     INQKSTEKNQKIIRDFLYKQISENKQSNILFDRSVIDNYIYSLALYDKGEASLEFLNE
                     TFDLVMRHLDFLDGVILIPTAASIKLVSDNLRDVDVNYIDKINRYFIKALLLINKTRP
                     LEVFVISGSREERIKLAGDIHRYMNYKMRL"
     gene            complement(25067..25489)
                     /gene="rplK"
                     /locus_tag="PLH96_00455"
     CDS             complement(25067..25489)
                     /gene="rplK"
                     /locus_tag="PLH96_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPB60282.1"
                     /translation="MAKKVEKKIKLQISAGKATPAPPVGPVLGQAGINIGDFVTKFNA
                     ATAQMGGMVIPVEISVYNDKSYDFILKTPPAANLLMKAAGVSKGSGKNLVSRAGTVTK
                     AQVKEIAEQKMSDLKASNLDAAARMIEGTAYSMGIEVK"
     gene            complement(25569..26114)
                     /gene="nusG"
                     /locus_tag="PLH96_00460"
     CDS             complement(25569..26114)
                     /gene="nusG"
                     /locus_tag="PLH96_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPB60283.1"
                     /translation="MARQELEQGRNWYAIHTYSGYENAVARNLKQRIESLGMEDKIFS
                     VIVPTEKKVKIKGGKRVEVEEKIYPGYVLVDMVVTDDSWYVVRNTPRVTGFIGSGVIP
                     VPLDEGEIKELFSRMESDSIKHKIELVKGEAVQIVDGPFKEFEGKVSDIDEERGKVKV
                     LVSMFGRETPVELDFLQVKRI"
     gene            complement(26149..26331)
                     /gene="secE"
                     /locus_tag="PLH96_00465"
     CDS             complement(26149..26331)
                     /gene="secE"
                     /locus_tag="PLH96_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPB60284.1"
                     /translation="MSKLTNYIKDTKSEMRHVSWPTKKQTINFTAIVIGVAVFTSALL
                     GVFDLIYQYILRFLLS"
     gene            complement(26474..27040)
                     /locus_tag="PLH96_00470"
     CDS             complement(26474..27040)
                     /locus_tag="PLH96_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPB60285.1"
                     /translation="MSRSIFAAGGAGQLEFLDEVIQTDAAINLGNSGGPLLNLRGEVV
                     GVNVATAIDSENIAFSLPINIVKPIIESVKKTGQIERPFLGVQYVQLMPETAEDLGIP
                     FDYGVVVSDNSGESAVVAGSPADKAGIKDGDIILELNERKIDESNTLARLIRDKKVGE
                     TVHLKIWRDDREIILPVTLGSVPADSGQ"
BASE COUNT         7702 a         6718 c         5213 g         7427 t
ORIGIN      
        1 ccggcaactt caacatctga ttcaccttcg ccaacttctt cattgctcaa tttggcaatg
       61 taatcggcgt cagagatggc accagcgcca ccttccaagt cggcatcatc ggcatcatcg
      121 tcatcatccg cggtaaccgg agttaccgga ttcaaagacg catacttagc gcggctgatg
      181 gaaccgaagt agccggcagc cggagcaaca ccgttgtcag cttgccactt agcaacagcg
      241 gccttggtca agttaccgaa gtaaccgtaa gcaacgccaa ctggcataac gaggtaaccc
      301 tcgtcttcca aatatttctg caggatttca acttgatcac cgcgagaacc aatggttagg
      361 tccttggtaa tctctccggc tgcagttgtt gaaccaccag tcaaagcagc aatctgcgct
      421 tgcaactggg cgatcaaagc tgtcaactct tcaactgtcg cggctttggc ggtagaaaca
      481 ccaaccaagg cgaagatgaa agcagcagct acaaccagag taagaattct tttactcata
      541 atataaattt gtaacttata aacttactta taatactaat aattgataat tttttatgtt
      601 ggttgttcgt acacctcgac accaacaggg aaactagttt aaaagttaga agttaaaagt
      661 caaaagttgg taagaataaa atccaaaacc aaaactaata aacttatcaa ctaacaaact
      721 actctccctg ttggagtcca agcaaacgaa ggcaaccacg aggagaatgt tcgaccaaat
      781 cccctcaacg cttaacctat ttttgtgaat ggaaaacaga aaattcagac aatgttcaga
      841 ttattaaatt ttcaaaggac gatacccaaa ttatagcaac tctaccgttg ttttcgcaaa
      901 agctctaatg tggataacta agcaagcggt tcaactagaa aacgcttgtt ttgctccatt
      961 agaacgtttt tttaatccgg ttgtgacaaa caactcaggt aatgctcatt atactaaaaa
     1021 tgaagcgaga gtcaataaac gttgaggtta gtggttaata gtcaaaaatt aggccgtaaa
     1081 ccaaagaaaa caaagatata ttaagggagc gggcagccag aagtggggct gaaaaagcca
     1141 agaattagat tggaatggat atagccaacg ttggccttaa tttgggccat taggccaaaa
     1201 gataacggct ccaacggcgg tccccttttt tcttaaggac ccctttttgg accaagtcgg
     1261 ccagctctcg ctgaatcgtt tttgtgctgc aatcagaaat agcgtcagag atgtccttaa
     1321 tggaagtcca gctcttgcct ttcaaaaaac taattatttc aagacgccga gcatctttgt
     1381 taactaagcg ccgatttttt ttacccccct ctttttggta taggtctttt tctttgccgg
     1441 cttctgtccc tttcagtctt tcagacaaca tttcgctcaa tcggacattg gcattatagt
     1501 ttgtctctga aatattatgt tccatagttg tcgcgactcc gccaaccgga tacttctctc
     1561 tcgcttccag cagcagttgt gaagcttctt ccggtccgac aatttccttt agacctcttc
     1621 ccagtttgga agagatgttt ttgcctaagc cccggaactc attttttaaa atcgccaaat
     1681 tcatctccga ggcaaagccg gcagctgaag caatgtccaa caaattcgcc acttggtcca
     1741 gacgattaag gacagcttcc aagactgaga ttttgaacaa gggagaaatt tgtccctctc
     1801 ccgaaccatc ggacaaactg gaggtcggta ccaaaagact ggtgtccgac atcagttcaa
     1861 ggacagattc ccgaattcgc cactttagag gttcgctatc agaaataaac gaagtaacca
     1921 aatacgtggc ctgaaccaac ttttttgatt taaggaaggc aaacttcaaa aagcgcgagt
     1981 ctattccttg gcccatatta ttgtccctta tattgttgtc tttttctata tcggccattg
     2041 taaattaatg tctttttggc attagtcctt tagtttttaa ggccattttg attctactcc
     2101 aaaaagaaag aaatgtccaa tagtcaattg tttggcctcg cttgtcgacc gagggaagaa
     2161 cggcggggac tcgccgaccc taattgccgt ttgcgcgcaa aaaagagacc accccgatct
     2221 taacaatcgg attctcaagt tatccacaag ttaaataatc cctgaatcac tcctgaaatt
     2281 tttgctcgcc tctgctataa ttgagatatg ggaagacaca aaaaaaaccg taatggcaaa
     2341 gaaaaaaaat cacggcgtga tgacgaaggt ctgccaagcc ttcacttaaa aaatgaaaca
     2401 aaaaaggcag tcctggcgat tatcttattc gccctgtcat tgttctttgt tttatcctgt
     2461 tttaacattg ccggcctggc cggtgtggtt acctataatc tcttttataa gttgttcggc
     2521 catggcgctt ttcttttgcc tgttctcttg gtcctcttgg gaatttacct gctccggtcg
     2581 ttagagttca agctttcgcc cagtcgcgtt attggtggtc taattttttt cctgtccagc
     2641 ttaagcatta tccaggtcat tgccggcgat accagtggcg gcgtggttgg cgggtttttg
     2701 gcctctcctc ttttacgcta ctttgacttt tatgccagct taatcttttt atccgcgctt
     2761 gttctcatct ctcttattat cacttttgat tctcatcaag tccttggctt tagtctctgg
     2821 caaaaattgt ttaagaaaaa ggaagaagaa gaggaacaag aagaaaaatt tgagacaata
     2881 aaattaagcg acgcttcaaa agaggccttg cgcgaagaat acccgacagc agaaacagat
     2941 aaagcagaca aagagccgaa caaagaaaaa gagctcaaag taaacgacgg cggattcctt
     3001 agcggggccc ctctctttgg caaaaaagcc aagacaacgg gcgcctttat tcccccgcca
     3061 atctcactct tagaacaaga cagcggcaaa ccaaacgccg gcgacatcaa ggccaatgcc
     3121 aacatcataa aacgaacttt gtccaacttt ggcattagtg tggaaatgga cgaagtttct
     3181 atcggtccaa ccgttacccg ttacgcctta aagccggccg agggaatgaa actctcccgc
     3241 attgtcgccc ttcagaacga cctcgcgcta tccctggccg cccatcctct ccggatagaa
     3301 gctcccatcc cgggcaagtc cctggtggga attgaaatcc ccaacaaagc taaggcgacc
     3361 attcgcttgg cctcacttct ctcgtcaaac gatttcgcgg aatcacatca tccgctttat
     3421 gtggctttgg gtcgcgatat ctcaggccaa ccgcgttttg ccaatctcgc caaggcccct
     3481 cacctcttga ttgccggcgc caccggctca ggcaaatcag ttaccattca cgccgtcatc
     3541 tcctctctgc tttatcgcaa ttcgccagaa aaaatgcgtt ttgtgatgat tgacccaaag
     3601 cgcgttgaac ttacgcttta caacaaaatt cctcacctcc tgaccccggt catcaccgac
     3661 tccaaaaaag cgattgccgc tctccgttgg ttagccaaag aaatggaccg ccgttacgat
     3721 attttggaag ccgaagccgt ccgcgacatc cagtcttacc acaagaacgt tctggaaccg
     3781 accttggcca aggcggctac caaaaaaaca accgccgaag agggtgaggc agatgaggcg
     3841 gacgaggttg aacaaatgcc ctacattgtc gttattattg atgaactagc cgatattatg
     3901 accgcctacc cacgcgagtt ggaagcggca attgttcgct tggcccaaat gtctcgcgcg
     3961 gtcggtattc acttggttct ttccacgcaa cgaccatcag tggaggtcat cactggtttg
     4021 attaaagcca acattccttc gcgaattgct ctacaagttg cttcccagat tgactcccgt
     4081 accattttgg atatggccgg cgccgaaaaa ctgctgggcg cgggcgacat gttgtatctg
     4141 tctggcgaaa tgtctaaacc ggttcgcctt cagtcggcct ttatttctga ggacgaagtc
     4201 aaacgaattt ctgcccactt ggccaagcaa tacgctgaca tcccgccatc cgaccttaac
     4261 ctgggagccg aaggaaactc gggtggttct cccctcttta acgccacctt tggcgattct
     4321 gatgctgacg atgacctata tgaagacgcg cgcgagtcgg taatccaagc cggcaaggct
     4381 tcagcctcgt atcttcaacg aaaattaaaa gtcggctacg cccgcgccgc tcgcctgctt
     4441 gatatgttgg aagaacgagg agttatcggt cccggcgagg gcgccaaacc gcgcgaagtt
     4501 tacggcgcgc caagcgcccc agaaatttta gagggagatg atgagaatat gatataatta
     4561 aattaattag aatgaccata gaaaccaaag gcttgaaaga aagaatttcc gccaaagcga
     4621 ttatcaccat tttggttgtg gcggttattc taacttactc cggcttcaag tttaaagatt
     4681 tgatttcggg accggatatt gtcctctact ctcccagcga tggctccagt ctgaataatg
     4741 agctggtcaa tatcaaaggt cgcgccgacc gaattactca aatctattta aatgggcgta
     4801 aaatttttac cgaccaagat ggcaattttg aagaaccgct cctcttgtcc tccggctata
     4861 atgcctttga actaaaggcc gaggataaat ttggccgaga aatagttcgg aagttgcagg
     4921 tcgtttataa caaaagttag caacccaaga cgaaaacagg gcaagacgca agatagggtt
     4981 ctgtagaaaa aagatccata gacattcaca aattagtatc gcgcttacat taggattatg
     5041 gctaaaaaaa caaaacaaac taaaactacc ggcaacaaca tcgccgacac catcaaggca
     5101 attcaaagca agtttggcga tgaagcaatt atgaaattgg gcgacaaacc caaggttggg
     5161 attgatgctg ttccaactgg ctcgttggcg ctagatgccg ccctgggtgt cggcggagtc
     5221 ccccgcggtc ggattataga aattttcggt ccagaatctt ccggcaaaac taccttggcc
     5281 ttgcatattg tggcccaagc acaaaaaaag ggcggggttt gcgcttttat tgacgcggaa
     5341 cacgcgcttg accctgaata ttccaaacgt cttggtgtcc aaaccgaaga attattaatt
     5401 tcgcaacccg attacggcga acaggccctg gacatcgccg agagcctggt acgttccggc
     5461 aagattgacg tggtcgtcat agactcggtg gccgccctga cccccaaaga tgaaatagag
     5521 ggtgacgttg gcgattacca cgttggcaag caggcgcgcc taatgtcgca agccttgcgc
     5581 aaaatgacct ccatcgtgga ccgcagcaaa acggtggtca tttttattaa ccaaatcaga
     5641 atgcaaattg gcgtgatgtt tggcaatccg gaaaccaccc ccggcggcaa ggcgctcaag
     5701 ttctactctt ctgttcggct agatgtccgt cgtattgctc aaattaaaaa aggtgatgaa
     5761 gttatcggcg gacgaatcag ggtcaaagtg gtcaaaaaca aagttgccgc gcctttccgc
     5821 caagccgaat ttgacctcct ctaccgtgaa ggcatctccc ccgagggcga gcttttggcc
     5881 attggcgaaa aatacaattt aattaaaaaa tccggcgcgg cttattctta cactccagat
     5941 ggcaaaacca ctcacgaagt gaagttgggg cgaggctatg acgccgccag acaattttta
     6001 cgcgaaaatt ccgaccttaa acaagaaatc ttgcaaaagg taaaggagaa actcttaaat
     6061 aacgacgagg attcttcaaa cgaagaaacc gacacaaacg acgaggaata gaagtctttt
     6121 ccggtctaaa ctttgtttgg ctggacaatt attcgcaatt cagttaatat ctgaaagtta
     6181 tgttggaata taatgaaata aaaccaagga agtttatcgt gtttgaagga gagccatttg
     6241 aagttttgac ttctcacgtt tttcgcaaac aacaacgcaa accggtcaat gccgtcaaaa
     6301 tgcgcaattt gattaccggc aaagtcatag aaaactcttt tcatcaaagc gaaaaggtag
     6361 aagaggccga acttgaacgc cggccaatca aataccttta tgaaaatcgt ggccaatact
     6421 ggttttgcga ggtcaatgac ccaagcaagc gttttgagat gccggctgaa actatcgccg
     6481 acaaaataaa gtttgtcaga caaaatgaca caattgatgt cttgtcgttt ggcgacaaag
     6541 tcctaggaat tgaagtacca atcaaagttg aactaaaagt gactgacgcc ccgccagcca
     6601 tcaagggcaa cacggtccag gggggtttga aacaaatcac cttagagacc ggcttcacca
     6661 ccaacgcgcc gatgtttatc aacgaaggag acactgtgcg aatcaatact gagactggcg
     6721 aatacgtgga acgagtctaa aaattaaaaa gcgttcagag gctgggacag ttttgacccc
     6781 aaattctgat ttaaataact taatcgataa gaacaaagac cccgcagagg ggtctttggg
     6841 tatgttttag aaaaggttac agactatcct tcaataaacg gtaacaacgc caaaaatctg
     6901 gcttgtttaa tcgccgtcgc cacttggcgc tggtgcttgg cacaagtgcc ggtgcggcgg
     6961 tgtttattga tacgtccatg agtatccaaa aaactcttta ggatttcaac atccttgtaa
     7021 tcaacaactt tggacttacc ggtgcaaaaa tgacattgtt tgttcatagg gccgtctttt
     7081 tattaactta actcttactt aactttttta tttttaatta tttgacctgt cgtgcttaga
     7141 taaacataat gctaacctac aaattcatgc gaatttacga ataaaaacta aaacgggata
     7201 tcatcgggat taatctcttc ttccggatat tgaatcgttt ccagctcaga tggggggacg
     7261 gacgccgatt caggcttgcc agccggcgcg ggactaccgg tgtcaaagga cgaaccgcct
     7321 tcgcgctttg gtccaaattg aaaactgtca acgattatct ctgttcgata tttcttgctg
     7381 ccgtcttgag cgtcccaact tctagtttgc atccggccct caatcattat gctgctcccc
     7441 ttcctcatat attgagcaat tgtctcagcc tgagctccga aggcaacaca gttatggaat
     7501 tgggtatctt cttgtctaac gccattctta tccttccaat aaagagtggt cgccaccgca
     7561 aagtttacca cggtcgtgcc cgatggcaga gctctcctct ccgggtcgcg tgtcaagttg
     7621 ccaatgacga aagctttgtt taaatacatg ataaaaaatt taaaattgaa aagttaaaat
     7681 gtaaaaacta agaagcctgt tcttcaagca agttttctat ttctttatct atatcttcct
     7741 tggtcatctc ttttacctct tccggttcaa cctctttttc tcgtctttca atctcaaagg
     7801 gttcggtttg aacctctcgt cgctgaaaag ttcgcttcgg caggacaatt gtctcctcgc
     7861 cttcaggcat cgcaatcgtc aaacaacgaa taattttgtc gcttttatcc aaaacgtcct
     7921 tcagaactgc cgccttttcc ggcaagagct taaaacgcaa agcgccaaaa taggcgtcat
     7981 taaaacgctc tttcttgttg ccaatatcct tttttatagt gtaagccaaa cgaatcattg
     8041 agggatccat ctcgctggtg attacgccgc caagcttgtc aattggggcc ttgagagtct
     8101 gaacgacatt ttcggccaag gcgtcttgag ggataatcgg ggtcaaaaga tagccgatct
     8161 catagatttt cggctcctgc tcttctccat tatccaaatt ttcagctttt tctatttcat
     8221 ccatacagag gccatattat cagcatctcg gacaaaagtc aacgacaaga acacgggcca
     8281 acacaagaaa agacgagcca taaatgaccg ggggaaggac aactctcccc tcaatttgaa
     8341 agagttaagc cctattttta caagctaaaa aattcttgag cattttgaac caaagctgcc
     8401 aaaaccaccg cgctcatctc tggccttagc tcggcgattt tttcagccac atatttgaca
     8461 taggccggct cgtttcgctg gccccgaaaa ggaactggcg tcgcaaaagg cgcatccgtc
     8521 tcggacatta agcgatttag tggcattttt ttgatgattt ctccatattg ttcagaaaaa
     8581 gttattactc caggaaacga caagtaaccg ccaagggcta agaatttttg cgccagcggc
     8641 caatcaccga caaaaaaatg catattgaaa cgaggcaatt tgccaaaatc ttgatactcg
     8701 cgcaagattt ggaacaagtc ttcatatgcg tcaaaagtcc cgactgatgg ccgacagtga
     8761 atcatcagcg gcttattcag aacgaacgcc aaatcaagat gttttttaaa caactcaacc
     8821 tgcgcctgac gttcagactc ttcggcgcaa tgaaaataat ccagaccaca ctcgccaatg
     8881 gcgaccacct tagggttttc ggccaaagct gtaatctctc caaaatcagc gagccggcca
     8941 tcggtcgggt gcagaccaac agagcaaaaa ccaccgccac caaaaaattc cgcttgttca
     9001 agcgcttttt gtgaagaagc caaatcagcg ccaatattca caaagctaac accttcttca
     9061 aacgctcgct cgcccacggc ttgccagtcc ttttcaaatt gcaagtttaa atgggtgtga
     9121 gcgtcaaaat ataagacagg ttttaggtta taggtgttag gtgttgagta aggcatagag
     9181 atgaaagggg gttttaggtg ttaggttata ggttgtagtt aagacttaag tttagaaatc
     9241 aaagaagata tcattgtaac caaactctca agtctctaaa ggatttaatg cgtttatctt
     9301 ccattactct ttaaactaaa acctgcaacc taacacctaa aacctgttta ttttctaaca
     9361 aacaacggtt cgggcatttt attgaccaaa attgcttgtt ttattttttc ggccgtttct
     9421 ggcaaaaacg gcgacaacat cactgccaaa cgcgccaaac gaaaaaccaa agttctcaca
     9481 atttttcgcg cctcttccgg ggcagtttta aaaagcttaa atggtgctcg ttcctggatt
     9541 tctcggtcca tttcccctat ttcgcgccat atcacattag ccgcccggtt taaatcataa
     9601 gctcccaagg catcattgta ttgttcccaa acactggaga tattccccac ggttgtgata
     9661 accgactcaa atgtatcttc ggtcgcgatc ggttcggcaa agtagttttc agacattttc
     9721 ataatccgcg ccgttaggtt gcccaaaccg ttggccaaat tggcgttgta agcttcttta
     9781 aacctctccc aagtaaaatc actgtcttca aaaatggaga actcgcgcgc cacctgataa
     9841 cgaaaagcgt ccacgccata ttccgccaca atctcaagag ggctaacaac attgccgaga
     9901 cttttactca tcttctgccc tccacttata atgaagccct ccaccaaaat aaccttagac
     9961 ggtggcaagc caacagacaa aagcattgct tgccacatcg ctgactgttg acgcaaatta
    10021 tctttaccgc aaaattgaac agctcctccc gattccaacc accagcggct aaaccgcgcc
    10081 tcatcatcag gccaaccgat agcggacaaa tagttgacca acgcgtcaaa ccacacatac
    10141 attacatgag ccgggtcgtc tggcaccgga atcccccagg gcattttgcc aaccaggcgc
    10201 gaaacgctga aatcctgcag tcccgccgaa acaaagtttt tgatttcatt taaccggctt
    10261 tttggcaaca caaaagccgg cttttgttca tacaattcaa gcagcgattt ctgatatttg
    10321 gaaaaacgaa aaaaataatt ttcttcctca acaatttgta attcaagggt tgggtgcaga
    10381 gggcatttgc cgtcttccaa ttccgattct tgcttttcca actcacaccc gacgcaatac
    10441 ttaattttat aaaatttttt ataaatatca tcattagccc gacaacgacg ccaaaactct
    10501 tgaaccgcgc tgatgtgatg ttcgtcagtt gtcctaataa aattcagctc aggagataga
    10561 ttgagtaaac tttgcaaacc tttaaacttt ttcgcccatt catcaacgta agcttgcggg
    10621 gttttgcctt cggcgagcgc tttttggtaa atcttttggc catgctcatc tgtgccggtg
    10681 ttaaaaaaaa cctcgtcccc caaaagacga cgccaccgcg caaaaatatc cgctcgcaca
    10741 atttcggttg caaaaccaat gtgaggcgcc gcgttaacat acggcaaagt cgtggtaatg
    10801 taaaatttag acgacattac ttaagccttt tttacgcttg ccaacatcgt taacatattc
    10861 catcaacaaa gttgttgccg gcaccgccaa aatcattccc aaaaagccgg ccaactcata
    10921 cccgacgacc aacgaaataa tgacaataat cggatttagg ccgataacct ttttcattac
    10981 caacggatat agcagatggt tttcaaactg ttggacaata ataaaaaaac caatgaccat
    11041 caaaccgaga gtaaaatttt ctgaaaaacc caaaaagacg gctgggacag ccgcgagaac
    11101 cggaccaaaa accggaatca attcaaacaa accggctgta atcgccaaaa ccaaggcata
    11161 cttgatgcca aaaagagtca ggccaatgta aaccatcgga ccaacaataa gaccaagcag
    11221 aagttgccct tgaatccagc ggccaatttt cacttccgtc ctttgccaca aattgataac
    11281 atactcttct tgcttgagcg gagttatcac gcgcaaaaaa ttctctacgc catttttctg
    11341 aactgcaaaa taaaaagaaa taacaataat caaaaagaag ctgacaaccc cattgaacaa
    11401 agatttgact aaatcataaa cattgccagg gatcggcgaa tccaaaagcc cgcgagaaat
    11461 tatctcctga attgaaaatt ccgtgtccaa actgtccgaa aaagagacaa tagaattcca
    11521 agccggatta ttgccgataa aggtgtttat ttgtttaggc aaagtatagg ccaagccggt
    11581 aatatcttga aaaactggcg gaacaaataa atacagaacc gagaacaaaa ttaggaaaga
    11641 caagaggtaa acaagcagga ccccaacaac tcgcggcaga ccaaaacgcg aaagccgagt
    11701 agccgctgga tctaccgccg aggcaatgac caccgccgtc agcaaagcca agaaaatatc
    11761 ccgcaaaaaa tatgatagga taatcaccgt taccaccacg gcgattttaa gaaccaccga
    11821 aatattaata ttaaaattta tttccttatt tagcataaac ttattatatc acgagttcgc
    11881 gcaaataatc atccttagac tgattggggc caactgccgc caaattaaga ctggttggtt
    11941 taaaaagatc ttgggccaaa ctccgaacat catccgccga taccgcgtta attttggcgg
    12001 caaattcccc cggagataaa atctcgcggc gatagatttc ttgcccacca aaaaagtaac
    12061 ccaaatcaaa agaagtttcc aaaccaagat aaagattgcc aattaaatgg tttttggccc
    12121 taattagctc ttcgtcagta accaaggagt tcttcaaacg gcggcattct tcagccatca
    12181 ctccaacaat ctgcttatct ttccgactgt ccgtaccagc ataaagcgca aagtaaccaa
    12241 aattggtgcg attgcttccg ctcgtgccaa cataataagc cgcccccaat tcttctctca
    12301 cccgttgcca taaacgagag ctagtgctgc ctcccaaaat gtttgaaaga accgacaacc
    12361 taaaatatcg cggatcataa atatcaaagc cgcgaaaacc catgacaatg tgagattgtt
    12421 cgatcggccg agtggcaatg gccaccttgg gttgagactg ggctaattct ttaaaaagcg
    12481 gtctgacatc acgcccagac ggcaagggaa caaatagttt ttccagctga gccttgactt
    12541 tttcttcttc aaaccggcca gagaccacta agacagttga atcggtcaga taatgacgac
    12601 ggcgaaaatt cattaagtca gaacgcttga agccggccac cgtttccttt tttcccaatg
    12661 tcgttcgccc ttgcggcgta tcaccatagg tcgctcctaa aaaaacatcc cacacaaaac
    12721 tggccggttt gtcttcataa ctcttgaatt cctccatcac tacccccttc tctttttcca
    12781 tctctgcttc atcataaatc ggatttaaat agatgtcggc caataaatct atcacctgaa
    12841 cagtcttatc cggcaccgcc gtgacataat aagaagtaaa gtcatatccg gtaaaggcgt
    12901 tataggtcgc gcctagagaa tcaaattctg aagcaatgat tttggcgttc ggcctcttgg
    12961 gagtgccctt aaaacccatg tgttctataa agtgcgccaa gcccgcctct tggtcaagtt
    13021 cgtttttagc gccagcattg gttaaaatca gaaagctaac tgacaaagcg tcggggcgcg
    13081 gcaagagaat gacccttatc ccgttggtta aaattgtttt ctttggtgtc attatttaaa
    13141 attaccactc aaattcctga ctgtcaaaat caggaacaac aataaaattt agctgaattt
    13201 ttagggccct tagaaagcca ggcctttggt agacaaataa gaaagaagct cggtttcttt
    13261 tactcgttct tgaacaccgg tatcgcgatc ccgcaccgtc accgcagcat cggtcaaggt
    13321 gtcaaaatca acggttaggc aataaggcgt gccaatttca tcttgtcggc gataacgctt
    13381 gccaatgttg ccgttatcat caaaaacaat tggctgaatt ttttcattta atttttcaaa
    13441 tatttccttg gccttagcca ccagttccgg tttatttttt aaaagcggga aaacagccac
    13501 tttgaccggc gcgagatttg ccttaagccg aagaaaaact cttgattctc cacccatctc
    13561 atcttccgta taagcgtcac ataacaccgc caaaagcaaa cgacctaccc cgcctgacga
    13621 ttcaataatg tgcggaataa atttttctcc actttcagtg tcctgataaa acaaatcttt
    13681 accggaaccc ttggcgtgtt gggtcaaatc ataatcacca cgcgcgtgga caccttccat
    13741 ttcatcaaag ccccagggat agttatattc aatatcataa gcctccttgg cataaaaaac
    13801 taagttctca tgcctcttcc aacgcagatt ttcttctctt aacccaaggt caagaaccgc
    13861 ctgccaacga tatgcccgaa gtttttcata ctccgccatt tcttgattgg gatgagtaaa
    13921 atactgcatt tccatctgtt caaattcacg agtccggaaa agaaactgtc gaggcgcaat
    13981 ttcgttgcga aaagccttgc caatctgagc aatgccgaag ggtaattttt tatgatttga
    14041 atctaaaaca tttttgaagt taacgtaaat gccctgacaa gtctcccccc gcaaataaga
    14101 cgggttgtcc agacctgact cggtaaaatc ccctaggttg gattttacca ataagttaaa
    14161 tctacgagca tctgtaaaat tttgtttgcc gcaattggga cattttatct cttgacgatg
    14221 ctcattgaaa agtttattga tttcttcttc agacattttt tcgtcagcaa aaactccgat
    14281 gtcgccaaga accttgtcca ctctgattcg agtgtgacat tctttgcatt ccgctaaagg
    14341 atccgaaaaa cctttggtat gacccgaagc ttcccaaatt ttaggattca taaaaatact
    14401 ggagtccaaa ccgacaatat cttgccgctt gtggaccatc cacttccacc aatgggtctt
    14461 tagattattt tctaactcca cccccatcgg cccaaaatca taaacaccag caaaaccacc
    14521 ataaatctcc gagctgggat aaacaaaacc gcgccgcttg gccagtgaca ccaccttatc
    14581 caaaagattt tctttttcac ccataataat tattataatt actctgttac cagttgatcg
    14641 ttacggccaa atttcgggtc attcgtcaag tcggtcgtaa cagcctctcc tgttttggtc
    14701 ataatggtat tggtaaatga atcagtcgct tgaaaatgag attgattggt cagtctaaca
    14761 agttggccga cttgactcaa gctcggagtg attgaaactt gaaaataagc ttccctaacc
    14821 ggcgcggtct cacccgtgcc cgccgacact tcaccaacct gccaactaac ctcccgggta
    14881 tttgggtcat aaattatttt ctcttctggt ggtgaaattg aaccagtcca attgacataa
    14941 gcgggcaatg tggttttcac ttttgtttgg tttagagtat tggttgtatt agtaatagac
    15001 caaacaatag tgtaggaagt atcctgcccg accctgggcg gcactggacc ggaattttta
    15061 aacggaccga cgctgtggat tgacctcgcg ctaaagtcgg cttctgtttc tatttttacc
    15121 aaccgctggc tgtcactggt tactttttcc gatagttggt cggcaccaac tctctggcct
    15181 tcaaaaaaag cggagattcc aaaactgggc tgactaaaaa cagcctcttg cgaaaaatca
    15241 agtaatttaa atttgaaaaa agaatttccg atttcatttg tatcaagctc cgacaagcca
    15301 ggcgtatttg tcctgtccca agtgatagta ttatcaattg agttataaaa accttcatta
    15361 gaaacaaccg accgcttatc tattaagttt tcatccaact taacaaccat tcgattatta
    15421 aataaacgat ccggcaggtt attcccccaa tttaagctga cagaaacttc atctctgggc
    15481 ctaatcacga cttctgacct agtacttcca tttatagaca agccaaaatt aataaacgaa
    15541 cgcttgataa ccgctttggc gaaagcttcg ccataatcaa aactatcttc atcgggactc
    15601 cctaggggcg agctgctaac acgcacgcga aaagacttta cgtcttcgtt ttggccttct
    15661 agaatcccat tgatttttat aacacgttcg ctaccggctg gaatgtcacc caaaaaccag
    15721 acactgcccc ccgccaaagg ctctggctcg gcatttttta acataaaacc ggtcgcggga
    15781 tattcaaggg tcaaatttat atttttcaac acagtttccg aattggaaac taattttata
    15841 accaaaggaa attcttggcc agcgttaagc tccgacggca aatcaagcga gaggtcaatc
    15901 ggtgaagcgc ccatcaccaa agtaaaggtt ttctccttag tgtaaatggc gttggaatca
    15961 ggtaaacgat attccatagt tacaacgact tcttgttcgc tgttacgttc accaaaaata
    16021 atcgcccgcg aagaaacatt aaccacttgt ttttcgctaa tccccccgat ttcttgttcg
    16081 tcaaccggaa aagcgctggc ccggtcaaga gcatttaagg ttccgaccgg ataagaaaaa
    16141 attacagaaa cggaatcaag aggggccttg ttgttgtttg taattgttgc ttgaaggttt
    16201 atctcatccc ccgcctttac gattgatgga ccttgaattt tcatttcaat attatgactg
    16261 gaaatagtgt tgaacccata aaaaaacagg taagcggcga ctccaagcgc caaaataaaa
    16321 aaaacaaccg aaaacaaaaa agtcctaaaa agaaaagagg aggtcaaggg cttcttttca
    16381 gatttagttt cttcggccgg aatgtcccaa tcggttttaa cgccaaactt ctttttgggc
    16441 aatagagttg attccgattc taacatttcc tcatcaggtt tatctctgta gatttttttc
    16501 tgcattgcct caagtcggct ggtaaaaccc gagctttttg gtgcttgttc catatttaaa
    16561 taatatcata aaataaccat aattacaggt gtgaattctg aagaacatca ttaatcgaac
    16621 gcaccgctcg aggcagatgt gtcttaaaat caaccaacgt atttttttgc caatctccgg
    16681 cttccgcgaa caagccaatc cctttatcgt cggaacgata accgaggacc gtcaacaacc
    16741 taacaactgt caggatttcc aagctttgtt gcaattcttt gccatcattg ctgttcttca
    16801 cccaggccgt caccgcgcct ttcaatattt catagaggtc cgggtctcgg ctctcgccgt
    16861 agataagacg cgataacaat gttgccgttt tcgccaagaa catctccagg ccatctggcg
    16921 cgcaatcggt gaccaagtta ttgaagttat tatccaattc cacatgaatc aagcgccaat
    16981 attcacgacc acgcaccaac gaaaccttta caaaacgtct aactgaggca ccgaaacgca
    17041 attttgactt ttccaaccta atcccctgcg ccaaacaatt caccaggcca aggtccggag
    17101 tcaaaatcct aaaaactttg ttggcttcgc cgtaaggccg gccactcaag ataaaacctt
    17161 cgctgtgata aattttgtag gacatcttct aatcatcttc accaattttt accttaaaag
    17221 ttttcccatt taccaatatc tcccagccct cttttacgat tccataatca agacgcttca
    17281 acccggcggc cttgattact tctttttcaa atcgacgtaa catttcccgt ccttcgtccg
    17341 gcgcctcaac acataaaaca attttgtcgt ttggcgtcag gcccaaagac ttcctgtatt
    17401 cttgcaggtt tctgataaat tcgcgggatt cgccttcttg cttcaaccca tcatccattt
    17461 ctgtgtccaa ccaaacctca tctgacaatt tcgggtcaaa aaccaatttt ttgacattca
    17521 actcttccaa aattaagttg tgatattcgg gtcgtaaagc caaatattga ctctttaccc
    17581 taagttctct aaggggttga cgcactttaa gaccggccgc ttgtctaaac tccaaagcca
    17641 gcgacacaat ttgacgaacg gcggccatct cttcgccaat cggcgtttcg atgtagacaa
    17701 gattattttc ctcattcggc cacttcgcca aatggacact ctctgactcg ctggcttttt
    17761 ttaatttgag ataaatctcc tcggcgacaa aaggacaaat cggagcaagc agtttggccg
    17821 tttctttaag cacgtggtag gttgtcgcga ttgcctcctc acggtccccc ttgtcgtctg
    17881 acttaaatct gtcacgcgag cgacgaagat accaaaccga taaatcttca ataaattctt
    17941 caatcggccg tgaggcacgg tcaatttcgt aattgtccaa tactcgctcc acctcaacga
    18001 ttgtcccccc caaacgtgac attatccacc tatcaagcac attcacaagt tggcgagaat
    18061 taaactcttt agtcggcaat tcctcggtgt aagtctcaaa gaaagatagg acgtttaaca
    18121 aacgagcaat gtttttgcgg tagatttccg ctatcccttt ttcagaaaaa ttaagatctt
    18181 ctgccctcac ggccggtgaa gacaccaagt aaaatctaac ggcatcagcg ccataacgat
    18241 ccaccaccac cattgggtcg ggataattct ttaatttctt agacattttt tgcccatctt
    18301 cggccaaaac catgccatta gcaatcacct gacgatatgg actctcccca aataaaccaa
    18361 tccccaaaac caacaagcta taaaaccagc cacgcgtttg gtcttgacct tcagcgataa
    18421 aatcagccgg aaaacctttg ccggttttag gctcgatccg cgaagtttca aaaggatagt
    18481 ggtcttgccc gtaaggcatt gcgcctgatt caaaccagca gtcaaagaca tcagggattc
    18541 ttctgagatt tccgccacaa gcgcaagaca acgcaaagtc atcaatataa ggccgatgaa
    18601 aatcaagttc aaaattttta ttatgtggca ccgacaagaa aggcacttcg cgaacttctc
    18661 cggtcgcaaa acctttttct ttctccatct cagctaacga caaatgacgc atagtagcag
    18721 ttgccaagaa aagaggcagt ccatggctaa caatcaggaa attcttgttt tgatatttct
    18781 cgtttaattc atagaccagc gccatcatcc gccggcggac catgttgtag ttttcaccgc
    18841 ccttttccgg ctgacgttca aacctttttc ttaaaggccc gacttgagaa tcgtattccg
    18901 cccaagtttt gcccaaaaaa ttgccggtat ttagctcgcg caacctttcg tcaaagacga
    18961 cttcatcctc acttaaccca agctcttcag ctaaaagctc cgctgtttcc ttggttcgca
    19021 caaaatcgga tgagacaatc atatccattt ttttctcttt cagtttttgg gcggacaacc
    19081 tcacctcttc tttcccttgt tcagtcaaac gatttgggcc attagggtca atactgatgg
    19141 catctctcaa gtttgattca gcctgaccat gcctcataaa tgtaaaggta ttgtttcggt
    19201 cttgttgcaa cttgattaaa tcggccaacg aaccaaccac ctccgtttgg ttgcaaacct
    19261 cacaacgcca aacgggcaat ggcgcgcccc agaaacgaga acgcgaaatt gcccaatcgc
    19321 gcgcgcccga gagccattta ccaaaacgac cctcgcgaaa atgcgccggc acccagccaa
    19381 ttccttgatt agcttcaagc aatttttctt ttaagtcagt aactttaacg aaccaggatg
    19441 aagcggcata attaagaaga ggtgtggcac aacgccaaca atgcggataa gcatgggtaa
    19501 ttttttcttt gctaaataaa aggttgcgag ttgccaataa tttaatgatt tcaatatcag
    19561 tcgcttgcga gttctctatt ggcttaactt tttgcccggc caattccccc atctccgact
    19621 taaactcacc agacatatta acgtgttgga taaacggcaa accttcctta agcgccaact
    19681 ggtaatcgtc ttcaccaaaa gccggcgcga tatggacaat gcctgtcccg ctttctgttg
    19741 tcacaaaatc agcttcaact atttgccaaa ccttggccac gccagcgatg tccttgcctt
    19801 tgacgtcttt taacggcaga tctttgtaat tatcaaatac cggtttatat tttttgccga
    19861 ttaaatctgc ccctaaaaat tctttttcaa gtttatattc cgcatcttta aaaacttccg
    19921 ccagcctgtc tttggcaaca atcaaaaccc cttccccttc tcctctgttt tgggcttcgt
    19981 ctttggcttt aggtctttgg cttttaactt gacaataaac aatctcttta cttaccgcca
    20041 aagccacatt tccgggcaaa gtccaaggag ttgttgtcca ggctaaaaga taaattcccg
    20101 gttcgtccac caactcaaat ttaaccgtgg ccgaaatatc ggtcacgtcc ttgtaaccct
    20161 gagtcacttc aaaatttgaa agggttgtct cgcaacgcgg acaaatgtgc atcgacttat
    20221 aaccttcata gattaaaccg cgatcgtaaa gcgttttaaa aacccaccaa accgtttcag
    20281 tatatgttga gtccattgtg cgatagtcat cctccatatc aacgaaacgg cccattcgtg
    20341 gcacgattct cttccactca tcggcataac ggagtacgct ttcgcgcgcc ttttcattaa
    20401 agccgtctat gccaaaatct tcaatgtctt ttttattttt caaaccaagc tctttttcca
    20461 ccaaattctc aacgggcaaa ccgtggcaat cccaacccca tcgacgacga acatgaaaac
    20521 ctttcattgt tttaaagcgc ggaatgacat ccttcatggt cccggccaac aaatgtccat
    20581 agtgaggcaa gccagtcgca aaaggtggcc catcgtaaaa tacaaattca ccctttggtg
    20641 aatctttggt caaagatttt tcaaaaattt gattctctcg ccaaaaagcc aagacttcct
    20701 cctcccgttt ggcaacgtca gatttttgag actcttcatt ttcagttaac atttccttaa
    20761 tttaccgcaa aaatggcttt atatcaacca accataaggc agaatgacgt tctgcccaaa
    20821 ccccaccctc ataattcaca aactctgaac aattcgccca gtcgcaacca ggttagccgt
    20881 tcaagccttt ctcaagacaa atttggaacc ttttacattt cctccaagac cgggatgcca
    20941 agcaaagtca gcccggactt gagcgtttgt gccaccgccc tggtcagaac caggcgataa
    21001 gcttcgtttt cactgccaat gattttgttc ttggcataat aagagttaaa acttgacgcc
    21061 aaattcacca agtaagtaac aatgtgttga ggcgccaatt cctggaaaga cctctccacc
    21121 accgccggca atttaatgag taatttttct atcaccatcg gttcttcgcc ttgagtaaaa
    21181 tcaacgattt ctttatccgc tttagccaaa accgatttgg ctcgagtgta agcatattgt
    21241 aaatatggcc cagagtctcc ttctaaggat agcgatctgt caaaatcaaa aacgatgtcc
    21301 cgccccggcg attgtttaag gatggaaaac ttaatcgcgc caattgccac cttttccgca
    21361 atctcttcct ttttttctac cggcagttca cgttcacttg ttttttccaa aaccctcttc
    21421 ttaacttctt caatcaaaga gacagccgtg atgacttcac cagtgcgcga agacatcttg
    21481 ccagttggca aacgcaacat cccatgagct acgtgccttg ccttggccgc caattccggc
    21541 tctaacaaat caagagcttt cagcataact ctgaaataat caagcacttc attgccagtt
    21601 accaaaaccg aaaggtcata cggatatctt tcaaatttaa gtttggccaa accaaggtct
    21661 tttgcttcat aagtcgacaa cccaagcgaa ttgacaaaaa cccgagtgtg catggactca
    21721 tcaaatttct cgcctcgaaa aacaatcgcc ccatcacttt tttcaaaaat acctttagac
    21781 aagccaacct ccaccatctt tttgccaatt ttttctgtct cgctctcaaa aaagtaaaag
    21841 tcaaaactgg tccccaaacg tttgtaaatt tccgcaaaat attcaagcga agcctgccgg
    21901 cccaaatcat aaacctggtt cgttttatca ttcgtccgtc catagacctg gcgattaata
    21961 tcattaattt ccttttttgt ttcctcatca gtctcgtaag ccttggatcc cgctacgtaa
    22021 aacttgcccc aatcgtgaat gtccaaattt tcttttttct ccgcttgaaa ataaccccaa
    22081 attgttttgg caacgtgtaa accaacatca ccctggtagc aagctcgttt tgtttcagct
    22141 ccgtttgcct cgattattct tgataaggct tcgccgattg agttactcat caagtggcca
    22201 atgtgaaact ctttaaacgg attggggtcg gtatattcca ccaccacttt tcgcccgctc
    22261 aatgtttggt tgcgaccata gttgtctccg gtctttaaaa tcgctcgcag actttctgca
    22321 aaaaatttct ctgataaata aaaatttaca aagccccctc ccgccacttg gacatctttg
    22381 atgttcggat tgttattaag acgaatttgt tcggcaattt tttcagccag gtcttttggg
    22441 tttaaccccg ctgtctttgc caccaccatt gccacgttgg tggaataatc gccaaattta
    22501 aagtcagccg gctgttccaa gttggtatcc aaaccgggga atcccacttc cagcgccatc
    22561 aacgcttttt ctatctccct ttttaactcc ttcctaatca tttttatatt atatagcatt
    22621 agccctctat ccccccaacg cgctcgttac tgttatttgc ccgtgctacc aaagccccct
    22681 tcgccgcggc ttgtgtctga cagttcatca acctcaatta attccggcga ttcaattctt
    22741 tgaattaaca tctgtgccac tttatcatgt ttttttatgc tataaatttc ctttgataaa
    22801 ttagtaacga caatctgaag ttcaccccga tagccggcgt ccaaaacacc ggccattgtc
    22861 ttgaggccat gattggccgc cagaccgctc ttgtcccaaa ttaaaccaac ataaccaact
    22921 ggcagttcta aagcaatgcc agttttgact cgtaccacct ctcccggttt aatctctgtt
    22981 tcggccaaag caaaaaaatc cattcccgcg tctcccggat gggcatagat tggcaatttg
    23041 gcttcagggt ttaatttttt aatcttgata atcataatgt tttggtcgcc gacaggataa
    23101 attttttcgt ttaattctag cttgtccgca tatccgtata ttaatgtcgt gataaaattt
    23161 tgtctatttt ttcagcaatc ttcaccatat ccttttccgt tttgccacga gtggtttccg
    23221 gcgcggtgcc caaacgcaaa ccagacgggt caaatggcga ccgagcatca aacggaatag
    23281 tatttttgtt cacaatgatt ccttcttttt ctaacagaag actcgcctct ttgccggcca
    23341 gtcgcccgcc agtggaagca acaacgtcca ctaacaacaa atgattgtct gtcccgccgg
    23401 aaacaattcg ccaacctttt ttttgcaatt ctttggccaa aactttggca tttttaacca
    23461 cttgcttagc atatttttta aactccggtt tcattgcctc cgccaaggcc accgcggtag
    23521 ccgcgatttg attgaggtga ggcccacctt gtaaaccagg gaaggccgct ctgtctattt
    23581 ttttatccag ttcacgttcg tcaattcgcg aaaaaatcaa accggccctc ggtccgcgca
    23641 aagttttgtg ggtagtcgtt gtgacaatat cagcataaag aaacggtgac ggatgaacac
    23701 cggccgccac caacccggca atgtgcgcca tatccaccat cagtaaggcg ccgcaaccat
    23761 ccgcaatttc gcgaaatttt ttaaaatcta taatccgtgg ataagctgta aaaccagcca
    23821 cgataatgtt tggtttttct ttttgggcta gtttttttat cgcctcataa tcaagctgtt
    23881 ccgtttttga gtccacacca taaggcacct gttgccaaac tttaccggtg accgaaactt
    23941 tgtggccatg agttaagtgc cctccttggt caagcgccat tcccataatt ttcccaccct
    24001 gaggcaccaa agccaggtac accgccagat tcgccggcga accggataag ggctgaacat
    24061 tgactgccca cttttttgaa cttaatttaa aaagttttaa ggcccttgac cgacacaatt
    24121 cttcaacttg gtctgtaatt tgatttccgc catagtaacg cttgcccgga tagccttcgg
    24181 cgtatttatt gactaacact gaaccaagcg ccgtcaaaac atctttggac acatagtttt
    24241 cggaagcaat tagattaact accttttttt gtcgtccagc ttcagccttg agcagttttt
    24301 caatttgttt gtctttcata atatgttacg aattgcaatt ataaccgcat cttataattc
    24361 atatagcgat gaatatcgcc ggctaattta attctttcct ctctgcttcc agaaatgaca
    24421 aagacttcca gaggacgagt tttgttaata agcaaaagag cctttataaa gtagcggttt
    24481 attttatcta tataattaac atcgacatcc cgcaaattgt cgcttaccag ttttatggaa
    24541 gccgcggttg gaatcaagat gaccccgtcc aaaaaatcaa gatgtcgcat aaccagatca
    24601 aaggtttcat ttagaaattc caaactagcc tcccctttgt catacagagc caaagaataa
    24661 atatagttat caataactga tcgatcaaat aaaatatttg actgtttatt ttcagaaatt
    24721 tgtttatata aaaaatctct aataatcttt tgattttttt cagtactttt ctgattaata
    24781 gagagcttcc tcttagaaat tacatcacgg taagtttttt gaggtgtttt gtaattggga
    24841 aaggctttta tgaagtcttt tataaaagtt gacttccctg tgttttgtgg accgataacg
    24901 gctaagcgca tatgttttat tttctcttat ttttaaaaaa cctcttatat cttagcaaat
    24961 ttacaatagt gaccaaaacc ctcaacaaaa aatgggggct ttttacaaac ccaggcacag
    25021 aaaaaccccc tttttgtggc aagttggggg tttccgggca agctatttat tttacttcaa
    25081 ttcccataga ataggccgtg ccctcaatca tacgggcagc ggcatctagg ttggaggcct
    25141 tcaaatcaga cattttttgc tcagcaatct ccttaacctg cgccttggta accgtgccgg
    25201 cgcggctaac caaattcttg ccagaacctt tagaaactcc ggccgctttc attaaaaggt
    25261 tggcagctgg tggcgttttt agaataaaat cataactttt atcgttataa acgctaattt
    25321 ccaccggaat taccatacct cccatttgcg ccgtggccgc gttaaacttt gtgacaaaat
    25381 caccaatatt aatacccgct tgacccaaaa ccgggccaac tggcggcgcc ggggttgctt
    25441 tgccggccga gatttgcaac tttattttct tttctacttt ttttgccatg atacaaataa
    25501 tcctaaccta taaatagcca tttgtaaata agagcaagtt aaaagaattt atagacgcct
    25561 tcgatgcgtt atattctttt aacttgcaaa aaatcaagtt caacaggggt ctcgcgccca
    25621 aacatagaga ccaagacttt gaccttgcct cgttcttcgt ctatgtcgga gactttgcct
    25681 tcaaactcct taaagggacc gtccacgatt tgaactgcct cgcctttcac tagctcaatc
    25741 ttatgtttga ttgagtcgct ttccattcgc gagaagagtt ctttgatttc cccttcatcc
    25801 aacggcaccg gaataacacc cgaaccaata aaaccggtca cccgcggcgt gttacgcaca
    25861 acataccaag aatcatcagt taccaccatg tctaccaaga catagccagg atagattttc
    25921 tcttccacct caactcgttt gccccctttg attttgacct ttttctcagt cggcacgata
    25981 acactaaaaa tcttgtcctc catgccgagc gactcaatgc gttgtttcag attacgcgct
    26041 acggcgtttt cgtaacctga ataagtatga atggcatacc aatttctgcc ttgttcaagt
    26101 tcttgtctag ccataattaa aaattaaaga ttaaaatgta aaatattatt aagaaagaag
    26161 aaagcgaaga atgtattgat aaattaagtc aaagacaccc aatagagccg acgtaaagac
    26221 cgcgacgccg ataacaatcg ctgtaaagtt gattgtctgt tttttggtcg gccaactgac
    26281 gtgtctcatt tcgctttttg tatccttgat gtaattggtt agtttagaca tattttttaa
    26341 attaggttat ctatatttta gcttattttc ttgtatttaa aaatgccccg cagggcttta
    26401 ttagataata gcccaaaagg tgtcgggagt caaccctaac gcagaaagat aggacaaagc
    26461 aagatacgcg accctactgc ccagaatccg ccgggaccga gccaagcgtt accggcaaaa
    26521 ttatttcccg gtcatctcgc cagattttga ggtgaacagt ctcccccact tttttgtcgc
    26581 gaatcaagcg cgccagagtg ttggactcgt caattttccg ctcattaagt tccaaaataa
    26641 tatcgccgtc ttttataccg gccttgtccg ctggcgagcc ggcgaccacc gctgactcgc
    26701 cgctgttatc ggagaccact accccatagt caaaaggaat acccaagtcc tcagctgtct
    26761 ccggcatcaa ttgcacgtat tgcacgccta aaaatggtct ttcaatttga cctgtttttt
    26821 tgaccgactc aataattggc ttcacgatat taattggcaa agagaatgcg atgttttctg
    26881 aatcaatagc ggtcgccaca ttcactccaa ccacttcccc acgcaaatta aggagtggtc
    26941 cacccgagtt gcccaagttg atggccgcgt ccgtttggat tacctcgtct aaaaattcta
    27001 gttggccggc gccgccagca gcaaaaatag agcgcgacaa accggacaac tagaattttt
//
LOCUS       DAOOLO010000004        22607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_2197_length_22607_cov_6.447277, whole genome shotgun sequence.
ACCESSION   DAOOLO010000004 DAOOLO010000000
VERSION     DAOOLO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 22607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22607
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2197_length_22607_cov_6.447277"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..918
                     /locus_tag="PLH96_00475"
     CDS             202..918
                     /locus_tag="PLH96_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB60286.1"
                     /translation="MQRRDSLIVGEYYHIYNRGVNKNLIFNDQNDYYRFIKSIFLFNS
                     DKSLEIGAVSRGNTYRNVFFVDRGNQLVDIGAYCLMPNHFHFLIKQKVDNGISIFFKK
                     VLTGHSMFYNKKYKRTGPLFEGRFKSKLISNDEYLRYLFAYIHLNPVKIQNPSWKDEG
                     IDDPSAAEAFLRDYRFSSYNEYANNKPRPEERIINKKEFPEYFDSCKEFRNFMNNWLN
                     YKEEFEILESEGVRRRTVLL"
     gene            1015..1500
                     /locus_tag="PLH96_00480"
     CDS             1015..1500
                     /locus_tag="PLH96_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60287.1"
                     /translation="MKKKIWLLVGLVILLLAIVWGVVWGPQTFSFYWYEYLNRKHLAE
                     FEALEEAYRMDTNGGETPEETLDLFVEALEAGDAGLAASYFVPEKRGEMEKQFKAGFE
                     SGGVELLLGEVKKEEEKHVLGYDIEGNSVRFEFSDGDSVGLTIDLIKNPNSQKWLIES
                     L"
     gene            1479..4031
                     /locus_tag="PLH96_00485"
     CDS             1479..4031
                     /locus_tag="PLH96_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60288.1"
                     /translation="MVDRKLVILGLFIFIGGLGNALPVWAYNDSTTHRAITQEAIKLF
                     EFYNPNQTFLEFEENLIIQGSEDEDEAPRWLNHFYDPVHNRGFKGGRSSKDWAQNPNA
                     QASRPGNGVINGYFSDPNDHSWPRAIYEYTWGDKERALLSLGHVLHLVQDATVPDHTR
                     DDAHAFKKTYEKYAERFTTKNFRLADDLAGRGQEPIRLDSLDEYFDNLAIFSNGNFFS
                     DHTILDDYYNRPIVRNQEFLELSDKKSHTFGIGDLEGVKLMEIDKEIDFLEGKFKETY
                     ILDDIDNLVASDYWQHLSEKAVLGSAGVIELFFKEVAAEKESRRLFDLNKSYLERFLG
                     RLAQGAKNTLAILSFWRTSPNNPQPTLVEDSSDGAEKLENSEQSVPEEKSQANKGTEI
                     NQVELARLMAEVQALQRQLAEYQTKLAIETGTNQENVLGEQEVKEIKEEDRRVHTSVS
                     GFLGGSSGGSSADENLISDEDEVVIAAPNILSPVDFSAPFSTATIIFSGTTTPGLTVF
                     CDFGHNNTTTSELDTGEWALEIIDLPQGTTTLNFFARDIENHISAPVAVEISVDSLPA
                     VISFGIEECKNSFFDDHCVISPVEKLNISWQASKGEYISYKLQEVWEFWEEAGEEVDI
                     KLATTASTSLMLDCDYPPYYPVGWRLVLTAENYDGEKIQSEPFEIWFDPWPLIINEIG
                     WTGTAASSTDEWLELYNPLPFEVNLEDFWIVGLDDKPRIELSGLIGGQSFYLIERNDD
                     LVISDLPADLVAGFGESPEAEGLPDDNLQLKLVKKVGETEVVFDQTPLFIPSYYWPKS
                     SIERRLWRVSGELQSNWIEAVTSKQQNGLDRNGEKIGGTPKLENSSRIFSLI"
     gene            4049..5473
                     /locus_tag="PLH96_00490"
     CDS             4049..5473
                     /locus_tag="PLH96_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPB60289.1"
                     /translation="MFLKQKILLGLFLTVFAFPFLVEADVIINEVGWLGTVESSYNEW
                     IELYNSGNEEIDLANWALYERDESGADVQIIKLTQKIAPQDYYLVVRVTASAPNPFPE
                     AEEAGTFKGGGILNTGENLVLKDLNGQEVDSVLALGQWRSSDRDGRTMQKNDANWIMA
                     MGTPGASNATEAISEGDGENDDNDNSGSGSSNNSNLSAHSGQVEISDYDPDDVEISAG
                     RARLTNVMTPIEFKVESKSTLAGIFEWSFGDGESKRGAKVSHKYDFPGQYNVVLNYYA
                     GNDEAVSRTTVTVTEPNFDLAVSPAEKFLAIKNLGDREENINDWTVKNGSSTVFTFAK
                     DTIIGAGSELKISLSKEITTKSWSLNCADGSVVAEVKTEISSGKLDKPILSASTTAKV
                     AELRQELVKAEMTLSRIQKERALSLAAVGSAGLTKPKPSDQQKIESLTSANLSPNSTD
                     SDIIIIEREAGVVTKILKAFKEFF"
     gene            5506..5631
                     /locus_tag="PLH96_00495"
     CDS             5506..5631
                     /locus_tag="PLH96_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60290.1"
                     /translation="MLIGFLAVLAVAVGVLFCLGFIDNTEEPSGPTNFAGLGEEE"
     gene            6376..6882
                     /gene="rplJ"
                     /locus_tag="PLH96_00500"
     CDS             6376..6882
                     /gene="rplJ"
                     /locus_tag="PLH96_00500"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPB60291.1"
                     /translation="MAITKDKKLEIVDKLKTIADKGKTIVFVNFHGLSVGGVNDLRQV
                     LRQKEVGYYVAKKTLIKRVLDSAGFSGEMPELEGEIALAYLTNEAGDTLLPIKEIYDF
                     QKKNAEVMKIVGGIYEGAFVGSDFVIQMAQIPNRETLYAQFVNLINSPIQGLVMALDQ
                     IAQKRETV"
     gene            7039..7389
                     /gene="rplL"
                     /locus_tag="PLH96_00505"
     CDS             7039..7389
                     /gene="rplL"
                     /locus_tag="PLH96_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPB60292.1"
                     /translation="MVDQVESMTVLELHELVKLLEEKFGVSAQAVMVAGGAAAGGEAA
                     EVKDSFTVTLTAAGDQKVQVIKAIKDKLGLGLKEAKDLVDAAPSVLKDGAKKDEADEI
                     KKAVEEAGGKVEVK"
     gene            7526..8167
                     /locus_tag="PLH96_00510"
     CDS             7526..8167
                     /locus_tag="PLH96_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60293.1"
                     /translation="MEILPKLFGGSNQVKLMRLFLFNPDNFFLLKELGQRTKIKPGQI
                     KKEIALLESIGLVKTRKVAGATPIKKTKKTKKGQKKKPARESLRSRLWGLNSEFFFVD
                     HLRSLFNADFFVSRDELAQKFKNCGKIKLLILSGIFVQEGGERVDMLIVGDGLKKKLI
                     EKIITGIESEIGRELTYAIMDSADFAYRFHSSDKFLRDILDYTHKKIIDKIGV"
     gene            8343..9029
                     /locus_tag="PLH96_00515"
     CDS             8343..9029
                     /locus_tag="PLH96_00515"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60294.1"
                     /translation="MYLQNWSDALAGSFQELITGIVTYVPNLVFAIILFIIGWVFGDF
                     IGLWVARLVRALKVDRILEKLGLHRLVEQAGYRLDAGKFLGFLIKWFIILGFLVASLN
                     VLKLDEVSNFLGEILAYIPSVIIATIILIIAAVVADVLQKVVVASARAARVSSAELFG
                     GITKWLIWIFAIVMALEQLGMMGPFLQIFMTGIVATVSIALGLSFGLGGKEAASNFIN
                     KLRGEISKRD"
     gene            9446..13683
                     /locus_tag="PLH96_00520"
     rRNA            9446..13683
                     /locus_tag="PLH96_00520"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            13723..13828
                     /gene="rrf"
                     /locus_tag="PLH96_00525"
     rRNA            13723..13828
                     /gene="rrf"
                     /locus_tag="PLH96_00525"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            13933..14697
                     /locus_tag="PLH96_00530"
     CDS             13933..14697
                     /locus_tag="PLH96_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60295.1"
                     /translation="MKWSSRRQLTYLTSVILFMALLVGAFYLIYKPAPTCFDQKQNQK
                     EEGVDCGGPCARVCQSVAVPLKTYWTRVFALGDGTYDVAALVENENQGLGIKRLAYTF
                     KLFDSNSVEVAKHEGSTFVNPGEKFVIFESRIKSSQSYPAVKAFVELEEYPVWEKISP
                     VPRIISIERVGFANEPYPVLRLKVKNDSFDNYRDIKINTVLSDVNQNAFAASGTFVED
                     LLAGESQEVFLTWPNSFVAEPSYTDSYWRINAFDLN"
     gene            14702..15832
                     /gene="rodA"
                     /locus_tag="PLH96_00535"
     CDS             14702..15832
                     /gene="rodA"
                     /locus_tag="PLH96_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPB60296.1"
                     /translation="MRWLLRQDSRIDWILFFSLLPLLIFGLVTMSSFTSESYFFYRQV
                     TWILLSLAIFFGFSLVDWRFLRRSGILVGGYLFLVGLLLLLFVIGTVTKGAQSWLSLG
                     GFSLQPADFMKLVLVLMLAKYFSKRHIEIANIRHIIVSGVYAFLPFVAIMLQPDFGSA
                     LIIFMIWLGMILVSGVSRKHLAWLGLVAIIFSVGAWMFVLQDYQKARIMTFIHPLADV
                     RGSGYNALQSTIAVGSGQLTGKGVGYGTQSRLKFLPEYQTDFIFAAFAEEWGFFGVLI
                     FFFLFGLIIWRILAIAMKGATNFEILFGLGIAVMFMAHLIVHVGMNIGIMPVTGLPLP
                     FLSYGGSHLLTEFMGLGILMGMRSYSLAYHRDDVKNEFIGPQ"
     gene            complement(15914..17155)
                     /locus_tag="PLH96_00540"
     CDS             complement(15914..17155)
                     /locus_tag="PLH96_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB60297.1"
                     /translation="MYNKYWSSTQYLNDFFLVRNIKKTKIFYLITKGNFGGAQRYVFE
                     LTQNLPLSHFEISVVMGEGNELRQKLERTGVKVRQLDTLGRDINLSADWQTFKTLFRI
                     LRQEKPEIIHLNSSKSGLLGALAGRLTGVPQIVFTAHGWSFNEKRSWLAKKVLLLLHW
                     LTIILCHRVIAVSQKTKQDMEKLPFVKNKINVIHNGLDNTKFFNRFVARRELTNYFPK
                     EIRRADESRFDLQTKFWLGTVSELHQNKGLDILIEAFANVVRKMLNSSLGWSLVLVII
                     GEGEERSKLQKLIRDKRLEGRIFLLGQVPEARNYLKAFDIFTLTSRTEALPYAAIEAG
                     AAGIPVIASEIGGLSEIIPGPEFGILTPPENIKEVEKSLLYMLKKPEYRKLAASNLKK
                     HVRENFSLKKMAAETIELYQR"
     gene            17258..18568
                     /locus_tag="PLH96_00545"
     CDS             17258..18568
                     /locus_tag="PLH96_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB60298.1"
                     /translation="MKLQTKKDAVVLLIGDLFLFGLALWLALVLRNFAWPEEGLFYRH
                     LQPFSIIFLIWVIVFYIAGLYGKHTAIFRRRLPRLIFNSLLANSAIAVVFFYFIPYFE
                     ITPKTILFLDLILSFIFIFLWRRYFAPNINLTQKEKILFVCRGAETSEIIDEIKNNPR
                     YSIQIAEPNDLRNIEESGITAVVVDLYDENIQEALKNFYNMIFSGIRFISVHNLYEEL
                     FDREPVNVIKEQWFLENISNRPKPVYDVLKRFTDLVFALGMGLVSLLIYPLVYLAIKL
                     DDGGVIFSFQERVGKDSQPIRLVKFRTMKIANDGAKWGEGKNEVTRVGKFLRKTRIDE
                     LPQLWNVLAGDLSLVGPRPEFADPVALYSQDIPYFKIRHIIKPGLSGWAQIHQDGEPH
                     HGISVSDTKTKFAYDLYYIKNRSFWLDVGIALKTIKILLMRKGI"
     gene            18570..19457
                     /locus_tag="PLH96_00550"
     CDS             18570..19457
                     /locus_tag="PLH96_00550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB60299.1"
                     /translation="MSKIIVTGGAGFIGSNLVDELVARGDEVHIIDNLSGGKQENLNP
                     AAIFHKEDITNFSVIRPLFDEVEYVFHLAALPLVQYSIEHPIETNEANVGGTLNVLTA
                     AKEAGVKKVVYSASSAAYGDQAVMPLVEEMPAKPKSPYGLQKYIGEMYCRIFSEIYNL
                     PTVCLRYFNVYGPRQNEEGAYALVIGKFLKQKREGLPLTITGDGTQTRDFVSVHDVAR
                     ANILAMEKEVGGGEVFNIGAGQNYSVNEVAKMIGGEVEYIPARLEPYVSQADNRKARE
                     VLGWTPTVSLAEGLKELTS"
     gene            19469..20251
                     /locus_tag="PLH96_00555"
     CDS             19469..20251
                     /locus_tag="PLH96_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPB60300.1"
                     /translation="MIIDGRQMAEEIKKELKEKLANKNLRLVIVSVGDNAVSAKYLAR
                     KQKFGEAVGVETIVHEFAGDVSEDELARHLAKLADDRSVNGVVVQLPLPEQLDTEKLL
                     TLIPSEKDIDALSPEAKVLPPVVAAIKEILERNSILVEGKKAVVIGRGRLVGRPVALW
                     LAQNGAEVEVVTLETKNPMAVIAQADLLVSGAGQPGLLKPEMLKEGVVLIDAATSEVN
                     NKLAGDAEAACADKCSLFTPVPGGVGPLTVACLFKNLAALSQ"
     gene            20400..21827
                     /gene="secD"
                     /locus_tag="PLH96_00560"
     CDS             20400..21827
                     /gene="secD"
                     /locus_tag="PLH96_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPB60301.1"
                     /translation="MNKTRLTAILLILAGLGLGFFVYYSEIPNTSFYRPFKLGLDLSG
                     GSRLTYVADVSQIDPSNISDAMSSLREVIDRRINVFGVSEPVVQVERSAIGGIDQNRL
                     TVELPGVTEVEEAVKMINETPTLEFRTERATTSPQRQAIIAAREEIQNYILDLPTTTP
                     AALIEEMLEEKFGQEKLNLVQEDPDFEPTELTGRYLEKAQVVFNQNSLGPSILVEFDR
                     EGSKIFADLTKNNVGKAVGIFLDGALISAPIVREEISDGQAEISGQFTVPEAKTLTRN
                     LNLGALPVPITLASTQVVGATLGEEAIQKGVKAGVLGLLIVAVFMILWYRLPGLVSVL
                     ALIFYIILMLSLFKLMPVTLTAAGMAGLILSIGIAIDANILIFERMKEEIAAGKGLLE
                     SIKEGFSRAWLSIRDSNLSSLISAVILFWFGSSLIKGFALTLMIGILVSMFTAITVTR
                     TLLLALGFRNRVGLAKFLFDSGFSK"
     gene            21918..>22607
                     /gene="secF"
                     /locus_tag="PLH96_00565"
     CDS             21918..>22607
                     /gene="secF"
                     /locus_tag="PLH96_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB60302.1"
                     /translation="MLIVKYRKIFYTLSLVLLALSVFAIARYGLNLGTDFIGGTLLEV
                     KYEEGRPELEELNSAVLDLELGSILIQPTGEQNYTLKLRNISETEKEALFEALTLGGK
                     KAFTQERLSEVGPTLGKELRNKGVVAITLVVLLIILYIAFVFRQVSKHHVKSWKYGVA
                     AIVALTHDIIISAGAMALLGHFSGAEADALFLTALLTILGLSVNDTIVVFDRIRENLH
                     RHPTVKFEEVVG"
BASE COUNT         6265 a         4165 c         5639 g         6538 t
ORIGIN      
        1 aacaaagacg aaacaatcta tctttacgac cccaccggca aactagttga tcaactagac
       61 tactaaacaa actattaaac aaattaacca catcctccaa ccctctagca gtctaaagga
      121 ggacggtcct cccttaggtt atccacaggg aggaccgtcc tccttgtcag tttttcttat
      181 taagtttaaa attaagacaa catgcaaaga agagattcgt taattgttgg cgaatattat
      241 catatttaca atcgaggagt aaacaaaaat ctaatcttta atgatcaaaa tgactattat
      301 cgttttataa aatcaatctt tttatttaac agcgataaat ctctagaaat aggtgcagta
      361 tcaaggggaa atacttatag aaatgtattt tttgtagacc ggggtaatca actggttgat
      421 ataggggcat attgtttaat gccgaaccat tttcattttt taataaagca aaaagtagat
      481 aatggcatat ccatattttt taaaaaggtt cttacggggc actctatgtt ctataacaaa
      541 aagtataaaa gaacaggacc tttgtttgag ggcaggttta aatctaagtt aatctctaat
      601 gacgaatatt taaggtattt gtttgcatat attcacttaa atccggttaa aattcaaaat
      661 cccagctgga aagatgaagg aattgatgat ccatccgcag cagaagcttt tttaagagac
      721 tatcgttttt caagctacaa cgagtatgcc aataacaagc ccaggccgga ggaaagaata
      781 attaacaaaa aagagttccc tgaatacttt gactcatgta aagaattcag aaattttatg
      841 aacaattggc taaattataa agaagagttt gaaatcttag agtcagaagg agtcagaagg
      901 aggaccgtcc tcctttaagt tatccacggg tttagaaagg ggaggggttt catgaagatt
      961 tttaggtggt aacattatta ataattttaa ttcataattt cataagttaa aaacatgaaa
     1021 aagaaaattt ggcttttggt gggtttggtg attttgttgt tggcaatagt ttggggtgtg
     1081 gtctgggggc cgcaaacctt ttctttttat tggtatgaat atttgaatcg caaacacttg
     1141 gcggaatttg aggcgctaga agaagcttat cggatggata ccaatggggg cgagactccg
     1201 gaagagactt tggatttgtt tgtcgaggca ctagaggctg gtgatgctgg gctcgcggcg
     1261 agctattttg tgccggagaa gaggggggag atggagaagc agttcaaggc tgggtttgag
     1321 agtgggggag ttgagctgct tttgggtgaa gttaaaaaag aagaggaaaa acacgtttta
     1381 ggatatgata tagaaggaaa ttctgttcga tttgaatttt cggatggaga cagtgttgga
     1441 ttaactatag acttgattaa aaacccgaat tcacagaaat ggttgataga aagcttgtaa
     1501 ttttaggcct atttattttt attggcgggc taggtaatgc tctgccggtt tgggcttaca
     1561 atgacagcac tactcatcgg gcgattaccc aagaagcaat caaattgttt gagttttaca
     1621 accccaatca aactttcctt gaatttgaag aaaatttaat tattcagggg agtgaagacg
     1681 aagacgaagc gcctcgttgg ctgaatcatt tttatgatcc agtccataat cgcggtttca
     1741 agggtgggcg atcttcaaaa gactgggcgc aaaatccaaa cgcgcaagct tctcggcccg
     1801 ggaatggagt gattaatggc tatttttctg atccaaatga tcattcttgg ccgcgggcca
     1861 tttatgaata tacttggggt gacaaagagc gagccctgct ttcactgggg catgttttgc
     1921 acttggtgca agatgcgacc gtaccggatc atacccgtga cgacgcgcat gcctttaaaa
     1981 aaacttatga aaaatacgct gaacgtttta cgacaaaaaa ctttcggctg gccgacgatt
     2041 tggctggcag aggtcaagag ccgattcgtt tggacagcct tgatgagtat tttgataact
     2101 tggcaatttt ttctaacggc aactttttta gtgaccacac aattttagat gactactaca
     2161 acaggccaat agttagaaat caagaatttt tggaactgag tgataaaaaa agtcatacat
     2221 ttggaattgg ggatttggag ggggtaaaat taatggaaat agacaaagag attgattttt
     2281 tggagggtaa atttaaggaa acctatattc tagatgatat tgacaacctg gttgcttctg
     2341 actattggca acatctttcc gaaaaggcgg ttcttggtag cgctggtgta attgaattgt
     2401 tttttaaaga agtagcggcg gaaaaagaat cccgccgttt atttgatttg aacaaaagtt
     2461 atctagaacg gttcttgggt cgcttggctc aaggcgctaa aaataccctg gccattttaa
     2521 gtttttggcg aacaagtcca aataatccac aaccaacatt ggtggaagac agttccgatg
     2581 gcgctgagaa attggaaaac tccgaacagt ctgttccaga ggagaaaagt caagctaaca
     2641 aaggaacaga aattaatcag gttgagcttg ctcgtctgat ggcggaagtc caggctctcc
     2701 aaaggcaact cgcagaatac cagaccaaac tggcgataga aaccggcacc aaccaggaga
     2761 acgttcttgg tgaacaagaa gttaaggaaa tcaaagaaga ggaccgccgg gtccatacca
     2821 gtgtttccgg ttttcttggc ggcagttccg gcggcagttc ggcggacgaa aatttaatca
     2881 gtgatgagga tgaggtggta attgccgccc caaacatctt gtcgcctgtg gatttttccg
     2941 cgccgttctc taccgcgaca attatttttt ccggtaccac tacgccaggc ctgaccgtct
     3001 tttgcgattt tggccataac aacaccacga cgagcgaact tgataccggc gagtgggctt
     3061 tggaaattat tgatttgccg caaggcacca ccaccctaaa ctttttcgcg cgggatatag
     3121 aaaaccacat ctctgcgccg gtggcggtag aaatctcggt tgattctctg ccggccgtta
     3181 tcagttttgg aattgaagag tgtaaaaata gtttttttga cgaccattgt gtgatttcac
     3241 ctgttgaaaa acttaatatt tcttggcaag caagcaaggg tgaatacatt tcttacaaac
     3301 ttcaagaagt ctgggaattt tgggaggagg cgggcgaaga ggttgatata aaattggcca
     3361 cgacggcgag cactagtttg atgcttgatt gcgactatcc gccttattat cctgtcggat
     3421 ggcgtttggt tttgacagct gaaaattatg atggagaaaa gattcaatca gaaccttttg
     3481 aaatttggtt tgacccctgg ccgttaatta taaatgaaat tggttggact ggcacagccg
     3541 cttcttctac tgatgaatgg ttggaacttt ataacccctt gccgtttgag gttaaccttg
     3601 aagacttttg gattgttggt cttgacgata agccacgaat tgaattgtcg ggtttaattg
     3661 gtggccaaag tttttatttg atagaaagga atgatgactt ggttatttct gacttaccgg
     3721 ccgacttggt cgccggcttt ggggaaagcc ccgaagcaga aggcctgccg gatgataatt
     3781 tgcagttaaa gctggtaaaa aaagtggggg agactgaagt tgtttttgac caaacgccct
     3841 tatttattcc atcttattat tggcccaaaa gttcaattga gcggaggttg tggcgcgtga
     3901 gcggagaact tcagtcaaac tggatcgagg ctgtgacttc taaacagcaa aatgggcttg
     3961 accgtaatgg cgaaaagata ggcggaacgc ccaaactgga aaatagcagt cgaatttttt
     4021 cactaatata aaaattacaa aattttttat gtttttaaaa caaaagattt tacttggatt
     4081 atttttaacc gtttttgcct ttccattttt agttgaggcc gatgttatta tcaacgaggt
     4141 cggttggttg ggaacggttg agagctctta taatgaatgg atagaacttt ataacagcgg
     4201 aaatgaagag atagacttgg ctaactgggc gttgtatgaa agagatgaat ctggcgcgga
     4261 tgttcaaata ataaaactta cccaaaaaat cgctccccag gattactatt tggtggtgag
     4321 agtcaccgct agcgcgccta acccatttcc cgaggctgaa gaagcgggca cttttaaagg
     4381 tggcggtatt ctaaacactg gcgagaattt ggttctcaag gatttaaatg gccaagaggt
     4441 tgattcggtt ttagctttgg gccaatggag gtcaagcgac agagacggga gaacaatgca
     4501 gaaaaatgac gccaattgga taatggcaat ggggacgcca ggggcgtcaa acgccacaga
     4561 ggcgatatcg gaaggtgatg gtgaaaacga tgacaacgat aattccggtt ccggatcaag
     4621 caataattcc aacctgtctg cccattctgg ccaagttgaa atcagcgact atgatcccga
     4681 tgacgttgaa atcagcgccg gtcgggccag gttaacaaat gtaatgacgc caatagagtt
     4741 caaggttgaa agcaagagca ctcttgccgg catttttgag tggtcgttcg gcgatggtga
     4801 gtcaaagcgc ggcgcgaaag tcagccacaa atatgacttt cccggtcagt ataatgtggt
     4861 tttgaattat tacgctggta atgatgaggc ggtctcgcgg acgacggtga cggtaactga
     4921 accaaatttt gatttggcgg tatctccggc cgagaaattt ttagctatca aaaacttggg
     4981 tgatcgggaa gagaatatta atgattggac cgtaaaaaat ggcagttcca ccgtttttac
     5041 ttttgccaag gatactatca ttggcgccgg gagcgaactc aagatttctt tgtcaaaaga
     5101 aataactacc aagtcctgga gtcttaattg tgccgacggc tctgtggtgg ccgaagtcaa
     5161 aacggaaatc agctcgggta aattggacaa gccgatttta tctgcttcaa ccactgccaa
     5221 ggtggccgaa cttagacaag agttggtcaa ggcggaaatg acgttgtcgc gaattcaaaa
     5281 agaacgagct ctctctttgg cggctgtcgg ttccgctggt ttgaccaagc cgaagccatc
     5341 agaccaacaa aaaatcgaat ccttaacctc ggctaattta tctccaaatt ctactgattc
     5401 tgatataata atcattgaga gggaggcggg ggtggtgaca aaaattttaa aagcatttaa
     5461 agaatttttt taatgtctaa atatctgaat tgtaattttt ggcggatgtt aatcggtttt
     5521 ttggcagttt tggcggtggc ggttggcgtt ttgttttgtc ttgggtttat cgacaatacg
     5581 gaggagccgt ccggtcctac aaattttgcc ggtctgggag aagaagagtg aaaatttata
     5641 aacgcaataa tcaagatgaa tcttgtggtg atgagagatt cgccttgctt ctatttactt
     5701 aaatttaatt tgtgatataa taaaacctgg atagaataat tacggaatag aggcaagcaa
     5761 aaaaaggagg agaagctatg ggcaatgaat atagtccggt ttctcatgcc tcaacttggt
     5821 cttttgtcgt gtgggcgaac ggggagattg attttgaagc ccgaagcgcg cacgactatt
     5881 gccttctcag aaaagggaag gcaaaaaagt gccggcagtg ccggcgacca attttggttt
     5941 ccaatctgga agcaggtctt tgccctgact gtgacggccg gggcgatgtc ctgggtttaa
     6001 atcgggggta aaaacacccc gaccaagctt tgcttggtcg gggtcaattt tttatccata
     6061 aataaaaaaa gattttcctt gagttttttg cggcgcaaac cagaatcttg tttgtattgg
     6121 gctgtttgac ttttctctaa aaatggtcaa taatctaaac agccgtagac agcaggtatc
     6181 gcaagagtaa aacctgccga ggggtcgccg ggaatactcg gttgacgtgt cgatccaagc
     6241 ggctattagc cgttttttta ttgtagaaaa ttaaggagcg aagcgactat aattttcaca
     6301 accccgcctt tcttaagggt ggggtggcag aaaagagtat ggcgaagcga ctataatttt
     6361 cacaaaaaat ctgttatggc aataaccaaa gataaaaaat tagaaattgt tgataagctc
     6421 aaaactatcg ccgataaagg caagaccatt gtttttgtaa actttcatgg cttgtcggtc
     6481 ggtggcgtta acgatttgcg tcaagttttg cgccaaaaag aagttggtta ctatgtggcc
     6541 aaaaagactt taatcaagcg cgttctggat agtgccggtt tttccggtga aatgccggaa
     6601 ttggaaggtg aaatcgcctt agcttatctg accaatgagg cgggggacac ccttttgccg
     6661 attaaagaaa tttatgattt ccaaaagaaa aacgcggaag tcatgaaaat tgtggggggt
     6721 atttacgagg gagcgtttgt gggctcggac tttgtcattc aaatggccca gattccgaac
     6781 cgcgaaaccc tgtacgcgca atttgtcaat cttattaatt cgccaatcca aggtttggtc
     6841 atggcgcttg atcagattgc gcaaaagaga gagaccgttt aaagcaaatt tagaaattat
     6901 taaataattt aaattactat ggacgaagaa aagaaagacg cgaccgcaga agtcgagtcg
     6961 caaacgccgg aagctccggc cgaaaaacca aaagaagaag taaaagccga tgttgttgtt
     7021 ccggccaaat tcaaggcctt ggtagaccaa gtggaatcaa tgactgtttt ggaacttcat
     7081 gaattggtta aattattaga agagaaattc ggggtttccg cccaggccgt aatggtggct
     7141 ggcggtgccg cggctggcgg cgaagcggcc gaagtgaaag acagcttcac agtcactttg
     7201 accgcggctg gcgaccaaaa ggttcaggtc atcaaggcga ttaaagacaa gttgggtctt
     7261 ggtttgaaag aagccaaaga cttagttgat gctgctccgt ctgttctgaa agatggcgcc
     7321 aagaaagacg aagccgacga aatcaagaag gcagttgaag aggccggcgg caaagtggag
     7381 gtcaaataat gtttttgaaa caaaaacggc ccatctcaag atgggttgtt tttgctttat
     7441 ttagaaagct ctaaataaga gttcccattt tttatcggtc aattttttga ttgcctaagc
     7501 ttcttctttg cggtatatta aaaatatgga aatattgcca aaattatttg gtggctctaa
     7561 tcaagtcaag ttgatgcgtc ttttcttgtt taatcctgac aatttctttt tgctgaaaga
     7621 gcttggacaa cgaacaaaga taaaacctgg tcaaatcaaa aaagaaatcg cattgttgga
     7681 atcaattggc ttggtaaaaa ccagaaaagt ggcgggggcg acgccaatca aaaaaaccaa
     7741 gaaaaccaaa aaaggtcaga agaaaaaacc ggcgcgagaa tctctgcgaa gccgtttgtg
     7801 gggtcttaac tcggaattct tttttgtcga tcatttgcgt tcgttgttta acgccgattt
     7861 ctttgttagc cgtgatgaat tggcgcaaaa gtttaagaat tgcggcaaga taaaattatt
     7921 gattttgtcc gggatttttg ttcaggaggg aggggagagg gttgatatgc ttattgtggg
     7981 cgatggccta aaaaagaagt taatcgaaaa aatcataacg ggcatagagt cggaaatcgg
     8041 acgcgaattg acctatgcca ttatggattc ggctgatttt gcctatcgct tccattctag
     8101 tgataaattt ttgcgagaca ttttggacta tacgcacaag aagataattg acaaaatagg
     8161 cgtttaattg cccgttgggt ccttttttga tatccacaaa attgctaaat atttcattaa
     8221 ttttgtgtta agataatgat aaggttttac cggtttatta gtcgaaccag cctcgtgata
     8281 tttgccaata acgaggtcaa taaaccaaaa attataagca aatttattaa taatacttag
     8341 atatgtatct acaaaattgg agtgatgctt tggcgggctc tttccaagaa ttgatcacgg
     8401 ggattgttac ctacgtgccc aatcttgtgt ttgccattat tctcttcatc attggttggg
     8461 tctttggtga tttcattggt ctgtgggtgg cgaggctcgt gcgagctctc aaggttgacc
     8521 gtattttgga aaaacttggc ttgcatcgct tggtagaaca agccggctat cgtttggatg
     8581 ccggaaaatt tctcggcttt ttgatcaagt ggtttatcat tctcggtttc ttggtcgcgt
     8641 cattgaacgt tcttaaattg gatgaagtca gcaatttctt gggtgaaatc ttggcttata
     8701 tcccaagtgt catcattgcg actattattt tgattattgc cgcggtggtg gctgacgtct
     8761 tgcagaaggt ggttgtcgct tctgcccgcg ccgctcgtgt cagttcggcc gaattgtttg
     8821 gcggtatcac caaatggctc atttggattt ttgccatcgt gatggccctg gagcaactcg
     8881 gtatgatggg tccgttcttg caaatcttta tgacggggat tgtggcgacc gtctctatcg
     8941 cgctcggtct ctcgtttggt ttgggaggca aagaagcagc ttccaatttc atcaacaaac
     9001 tgcgtggtga aatttctaaa agagattaag cctaaaagtt caaccaaaac attaaaaaat
     9061 gccctaattt agggcatttt ttaatgtttt ggttgggggt aacagaagct atcctttgag
     9121 ctaaattctt gtttgacata tattttttat tcgtatatac tattaactgt cagtatggat
     9181 attaagaaaa attccaaaat taagcggaaa ctgtagcccg attgttttgg tttggctcca
     9241 gaccgcttga ggcaagcggt ttttttgttc aagtattact ttgaacaatc ccgaatgact
     9301 gaactttgaa aattgaatgc caccatcaga caagcgaggg gtcttgcggt ttgagaggtg
     9361 gaaaaaaggt agatctgcgc tggtgcaaag agcgcagaac acaaaatgta agtataaagt
     9421 aaataaaatg gtttttaaat ttcctaatgg gaaattttaa gagcataggg tggatgccta
     9481 gactctaaaa ggcgatgaag gacgtggctc ggctgcgata agcttcgggg aggtgcctag
     9541 caacctttga tccggaggtg tccgaatggg gaaacccgat atggcaaaac catatcattt
     9601 gataacttga ttatcagatg cgcacccggg gaattgaaac atcttagtac ccggaggaaa
     9661 agaaaaaaag tcccgctttg tgggaaacat tctcttagta gcggcgagcg aaaagagaag
     9721 agcccaaatc tctcacccag aatgactttg ttccacgttg tttattaaag tttatataaa
     9781 attttaataa tggttgataa tttaaaatta ttgctcaacg tgaagtggaa caaaaaacaa
     9841 cgaagttatt ctgggtgagg gaggttgtga gacagcgatg tagtttacta gaagagttac
     9901 aaaatattta tttaattgaa tttgctggga agcaaagcca tagaaggtaa cagccctgta
     9961 agtgaaaaat agatatctct tttatcgctg atctcgagta cttcgaggaa cgacagcctt
    10021 gagggaatct ggcagaacta actgctaagg ctaaatactt ttagagatca atagtgaaca
    10081 agtaccgtga gggaaaggtg aaaagtagcc cgggagggcg ttgaaataga acctgaaacc
    10141 ctatgcttac aaggagtcgg agtctcttac ccagaatgtc tttgttccac gttgtttatt
    10201 gaaagttaga aattaatttt caatattggt gataattaaa aattaccgcc caacgtgcag
    10261 tggaacaaaa aataacgaag acattctggg tgagggatga cggcgtgcct attgaagaat
    10321 gagccaacga gtttatgcat attgcttagc taagcctcaa aagggtggag ctgtagggaa
    10381 accgagtatg aaagtgcgaa ctttagaagt tggaggttgg aagttagaag ttgggtttcc
    10441 caacgtctaa tatctaactt ctaatttcta ttttctgtaa tatgcataag acccgaaact
    10501 gggtgagctt gccatgggca ggatgaacct cgcagaaatg cgagaggagg tccgaaccgg
    10561 ttagccgtgc aatactatcg gatgacctgt ggtaaggagt gaaaagctaa tcgaactcag
    10621 taatagctgg ttctctccga aatagcttta gggctagcgt ctagcatacc ctctgggggt
    10681 agagcactgg aagagttgaa tagggagaaa tctcgtcaac tctatcaaac tccgaatacc
    10741 agaggtcaag tactaggcag ttagacggcg ggggcgaagc tccgttcgtc aaaagggaaa
    10801 cagcccagat ctcaatctaa ggtccctaaa tcaatactaa gtgcacttaa ggaggtgaga
    10861 tttctctgac aatgaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
    10921 ctcactcatc aagagatttt gcgccgcaaa ttatcggggc tcaagtattg taccgaaggt
    10981 gaggatgcta gtgcttggca ctagcgtggt aggagagtgt ttttcgctgc tgtgaaggtt
    11041 tgccgcgagg ctgactggag cgcgaagaag taagaatgtt ggcacaagta accgcaaccc
    11101 cggtgagagc ccggggcgcc gaaaaaccaa ggtttccttg gcaatgttga tcagccaagg
    11161 gttagtcggg cctaagccga tggcgaaagc cgcaggcgat ggacacatgg ttaatattcc
    11221 atgacctctt tatttttccg atggagggac gaaggaaaag agtgaatgcg ccttatagga
    11281 ttggcgttgc tactcctagg aatgctcttt aggtaaatcc ggagagctga tttaattcaa
    11341 attccgagag cggcaaaatt cctttctacg gattgggaaa ttttcacgca gagccttccg
    11401 agaaaatctt ctagggttaa gaataaagag tccgtaccgt aaaccgacac aggtggttag
    11461 gtcgagaaga ccaaggcgaa cgggtgagag atcctcaagg aactcggcaa aaaagcggcc
    11521 gtaagtttgc aataaggcct tcccacaccc agaatgactt tgttccacgt tgtttattaa
    11581 agtttgtata aaattttaat aagtattggt aatttaaaat tattactcaa cgtgaagtgg
    11641 aacaaaaaac aacgaagtca ttctgggtgc gggacgcagc taaagtatcc ctggcgactg
    11701 tttatcaaaa acacagctcc ctgcgaactc gtaagagtat gtataggggg tgacgcctga
    11761 ccaatgccag aaggtcaaat aaggctggtg agattatatc aagctggttt tataagccct
    11821 ggtgaatgtc ggcgataact ataatcgtcc taaggtagcg taattccttg tctggtaagt
    11881 tcagacgtgc acgaatggcg taacgactgg ggaactgtct cgaggatttg cccggtgaaa
    11941 atacagtggg ggtgaagatg ccctctacct gcagctagac gaaaagaccc cagaagcttt
    12001 actacaactt gatattgggt atacgtttta tctgcgtagc ataggtggga gaggttgaag
    12061 cgacggtttt ggctgtcgtg gactcgtcag tgaaatacca ctctggttga gtgtatattc
    12121 taacctgacc ggcaaaaacg gtcggagaca gtatctggtg ggtagtttta ttggggcgat
    12181 atcctcctaa aaagtaacgg aggagtctat taaggtcggc taggcgcgaa tggaaatcgt
    12241 gccgatagtg taatggcaca agccggctta actgcaagac gtacgtgtca agcaggtgcg
    12301 aaagcagagc atagtgaacc gacggtccgt attagacgcg gccggagatt aacggataaa
    12361 agctactctg gggataacag gctggttccg cccaagcgtc catagcgacg gcggagttcg
    12421 gcacctcgat gtcggctcat cttatcctgg gggtgaagaa gctcccaagg gtatggctgt
    12481 tcgccattta aaaagatacg cgagctgggt tcaaaccgtc gtgagacagg ttggtctcct
    12541 atctgctgca ggcgttgata attgagaaga tctgcttcta gtaagaaatt gctacttggc
    12601 tcctagttcg gttaaagccg gatggacccg atccgcctat ggtggattta gggaaagaaa
    12661 tttgccatga caatttggct aataacggtg aagccttcat aaatgatttg gcaactggat
    12721 aatattggcg ttggtccgta caatattgta taattacttg aggaccaaat tatttttaag
    12781 gtaataccgt gggaagtcga tctaaaattg attttgctta tgtcgccggg tttctggatg
    12841 gcgacggaag cttgatgttg caactcaaaa agagaagcga cagtccaaag aagtaccggt
    12901 ttatggccac tgtttgtttt tatcaagaca gtcgccacaa gaagacactt ttttggattc
    12961 gtgatctttt tggggttggt tatgtttcgg atagaaaaga tggaatgagt gaacttcgaa
    13021 tcaatggatt cgtctctgtt caacttgttc tggttaaact cttgccgttc ataaagttca
    13081 aaataacaca ggctagggct ttggctcagg cttgcgagat tttgaacaaa acaacattaa
    13141 gaacaataag cgaaaaacag ttaagaaaac ttgttggatt gattttggtg attcaaaatg
    13201 aaaattatgt taccaaaaga aaaaagacag gaactgaact acttaagatt ttaggtttga
    13261 ccccgtaacg acttgtcctg aaagatggac agacttgtat gatggcgaat cgtttctttg
    13321 aaaacgacgg ctcatatggg taatatgcca acccttcgtc cgctttacag aaagcggaaa
    13381 aggtgaagat atagtctgcg ctcctagaaa taggggaata attcttaacc aaaatttttt
    13441 ggttgaatga agcgacgaga ggaccgaagt ggactgacct ctggtctgag agctgtcctg
    13501 ccaagggcac tgctctgtag ctaagtcggg aatggataac ctctgaaagc atctaagagg
    13561 gaagccaact tcaagattaa ttatcgttat caggctcctg gaagattacc aggttgatag
    13621 gccttaggtg taagcgtagc aatacgttga gccgagaggt actaattcgc cgattcctat
    13681 taggaaattt gaaaaccatt tattttattt cgaatatttt gttctggtgg tttgacgccg
    13741 aggtcacacc tcttaccatt ccgaacagag aagttaagct cggttgtagc gatggtactc
    13801 gcaaggggag agtagctaac cgccggaaca aagtatttga attctaatct accaaccttc
    13861 cttttcccaa aacggtctaa gccgtttttt gggaaactgg caaaacttga tgagtggtgt
    13921 tattattttg atatgaaatg gtctagccgc cgtcaattga cttacttaac gagcgtgatt
    13981 ctctttatgg ctttgttggt gggcgctttt tatttgattt acaagccggc cccaacttgc
    14041 tttgaccaaa aacaaaatca aaaagaagag ggggttgatt gtggcgggcc ttgcgccagg
    14101 gtttgccaga gtgtcgctgt tccgctaaag acttactgga ctagggtttt tgccttgggc
    14161 gacggaactt atgacgtggc ggctttggtg gagaatgaaa atcagggctt gggcataaaa
    14221 aggttagcct acacttttaa attgtttgat tcaaacagtg tggaggtggc caaacatgag
    14281 ggctcgactt ttgttaatcc gggtgaaaaa ttcgttattt ttgaaagtcg cataaaatcc
    14341 agccaatcat acccggccgt caaggctttc gtagaattgg aagaatatcc ggtttgggaa
    14401 aaaatatcgc cggtgccgag gattatttcc atagaaaggg tgggttttgc caacgagcct
    14461 tatccggttt tgcgcttgaa ggtcaaaaat gattcttttg ataattatcg ggatataaaa
    14521 ataaatactg ttttgtctga tgtcaaccaa aatgcctttg ccgccagcgg aacttttgtg
    14581 gaagatttat tggccggcga gagccaagag gtatttttga cttggcccaa ttcttttgtg
    14641 gccgaaccat cttatacgga cagttattgg cgaataaacg cctttgattt aaattaaatt
    14701 tatgcgttgg cttttacgac aagacagccg gattgactgg attttgtttt ttagtctttt
    14761 gccactcttg atttttggct tggtcacgat gagttctttt accagtgaga gttatttttt
    14821 ttatcgtcaa gtgacttgga ttttgttgtc cctggctatt ttttttggtt ttagtttggt
    14881 tgattggcgt tttttgcggc gaagtgggat tttggttggg ggctatcttt ttttggttgg
    14941 tcttttgctc ttgctttttg ttattgggac ggtaaccaag ggggcgcaaa gttggctttc
    15001 gctcggcggt ttttcattgc agccagcaga tttcatgaag ttggtgttgg tcttaatgtt
    15061 agccaagtat ttttccaaac gccacattga aatcgccaat attagacaca taattgtttc
    15121 cggtgtttac gcttttttgc cattcgtggc aattatgctt cagccggatt ttggttcggc
    15181 tctgataatt tttatgattt ggttagggat gattttggtt tctggagttt ccaggaaaca
    15241 tttggcctgg cttgggctgg tggcaatcat cttctcagtt ggggcgtgga tgtttgtttt
    15301 gcaggattat caaaaagcgc ggataatgac ttttattcac cctttggccg atgttcgtgg
    15361 gtccggctac aacgccctcc agtcaacgat cgcggtcggt tccggtcagt taaccggcaa
    15421 gggggtgggt tacggtaccc aatcgcgact taagtttttg cctgaatacc agaccgactt
    15481 tatctttgcc gcttttgccg aagaatgggg cttctttggt gtgctcatct tcttcttttt
    15541 atttggtttg attatttggc ggattttggc tattgccatg aagggggcga ccaactttga
    15601 aattttgttt ggtttgggaa ttgctgtgat gtttatggct cacttgattg ttcatgttgg
    15661 aatgaatatc ggcattatgc cggtgaccgg cttgccattg ccatttttaa gttatggtgg
    15721 ctcgcattta ttgactgaat ttatgggctt gggtattttg atggggatga ggagttactc
    15781 gttggcctat catcgcgatg atgtgaagaa tgagtttatc ggaccgcaat aacgcagact
    15841 taagcagata aaacgcagat ttagcaaaaa ggagaggcaa gacctctcct tttacagtag
    15901 acgcgttgtg attttatcgt tggtaaagtt caatagtttc agcggccatc tttttgagag
    15961 aaaaattttc tcggacgtgc tttttgaggt tgctggctgc cagtttgcga tactccggtt
    16021 ttttgagcat gtagaggagg gatttttcca cttctttgat attttccggc ggagtcagga
    16081 tgccaaattc cgggccggga attatttcgg ataggccgcc aatctctgaa gcaattaccg
    16141 gaatgccggc tgcgccggct tcaatggccg cgtaaggcag ggcttcggtc cgtgaggtga
    16201 gagtaaaaat atcaaacgct ttcaaataat ttctggcttc tggcacttgt cccaataaaa
    16261 agatacggcc ttctagtctc ttgtcgcgaa ttagtttttg caattttgac cgctcttcgc
    16321 cctcgccgat aataaccagg acgagcgacc agccgagaga actgttcagc atttttctaa
    16381 caacgttcgc gaaagcttca atcaaaatat ctaaaccttt gttttggtgg agttcggaaa
    16441 ccgtgccaag ccaaaacttg gtttgtaaat caaatcgcga ctcatccgcc cgtctgattt
    16501 ctttggggaa ataattggtt agttcgcgcc gcgcgacaaa gcggttaaaa aacttggtgt
    16561 tatccaggcc gttatgaatt acgttgattt tgttttttac gaagggcagt ttttccatat
    16621 cctgcttagt tttttgcgaa acagcgataa ctcggtggca aaggatgatg gttaaccaat
    16681 gcaacaagag aagaactttt ttagccagcc aggatctctt ttcgttaaaa gaccagccgt
    16741 gggccgtaaa aacaatttgt ggcacgccag tcaagcggcc ggccagagcg cccaacaggc
    16801 cgctctttga gctgttcagg tggattatct ctggtttttc ttgacgcaga attctgaaca
    16861 gagttttgaa ggtttgccaa tcggcagaga gattgatatc acgtccaaga gtgtccagtt
    16921 gccggacctt gactccggtc ctttccagtt tttgccgaag ctcatttcct tcgcccatta
    16981 ccaccgagat ttcaaaatga cttaagggga gattttgggt tagctcaaaa acataacgct
    17041 gggcgccgcc aaaattgcct ttcgtaatta gataaaaaat ctttgttttt ttgatgtttc
    17101 gcacaaggaa aaaatcgttc agatattggg tggatgacca atatttatta tacatttttt
    17161 tcgcttattt tctacttttt gtttgcctct ttttaaaccg cttaaacagc ggtcagggtg
    17221 gcaaaaaaac gatttttgct gtattatcaa ggcaaagatg aaactgcaaa caaaaaaaga
    17281 tgccgtggtt ttgctaattg gcgacttgtt tcttttcggg ctggctttgt ggttggcgct
    17341 ggttttgcgt aattttgcct ggccggaaga gggacttttt tatcgccact tacaaccatt
    17401 ttcaatcatc tttttaattt gggtcatagt tttttatatt gccggacttt acggcaagca
    17461 tacggctatt ttccggcgca ggttgccgcg cttaattttt aactcgcttt tggcaaatag
    17521 cgcgattgcc gttgttttct tctacttcat tccatacttt gaaattacac cgaagacaat
    17581 cttgtttttg gatttaattt tgtcattcat ctttattttc ttgtggcgtc gttatttcgc
    17641 gcccaacatc aatttgacac aaaaagaaaa aattctattt gtttgtcgcg gcgcggagac
    17701 ctcggagata attgatgaaa taaagaacaa tcctcgttat agtatccaga tagccgagcc
    17761 aaacgaccta agaaacatag aagagtccgg tatcacggca gtagtcgttg atttgtatga
    17821 cgagaatatc caggaggcgt taaaaaattt ttataatatg attttttcgg gcattagatt
    17881 tatttctgtt cacaatcttt acgaggaact ttttgaccga gaaccggtaa atgtgatcaa
    17941 agaacaatgg tttctggaaa atatttccaa tcggcccaag ccagtttatg atgttctaaa
    18001 aagatttacg gatttggtct ttgcgctggg gatggggctg gtttctttgt tgatttaccc
    18061 ccttgtttat ttggcgataa aacttgatga tggtggtgtc attttttctt ttcaagaaag
    18121 agtgggtaag gatagccagc caattcgttt ggttaaattt agaacgatga aaattgccaa
    18181 tgatggcgcc aagtggggcg aaggcaaaaa cgaggtgacc agagttggaa agtttttgcg
    18241 taaaaccaga atagacgaat tgccacaact ctggaatgtt ttggcgggtg atttgtcgct
    18301 ggtgggaccg cgtccggaat tcgccgaccc ggtggctctc tatagccaag atattcctta
    18361 tttcaaaatc agacatatta ttaagcccgg tctttccggt tgggcgcaaa ttcaccaaga
    18421 tggcgaaccg catcacggga ttagtgtcag tgataccaaa actaaatttg cttatgacct
    18481 gtactacatc aaaaatcgca gtttttggtt ggatgtcggc attgccttaa agacaattaa
    18541 aattttatta atgcgcaagg gtatttaata tgtcaaaaat tattgttacc ggtggagccg
    18601 gttttattgg ttcaaacttg gtggatgagc tagtggcaag aggagacgag gttcatatca
    18661 tagataatct gtcaggaggc aaacaagaaa atcttaaccc ggccgccatt ttccacaaag
    18721 aggatatcac caatttttcg gtcatcagac cattgtttga cgaggtggaa tatgtctttc
    18781 atttggcggc cttgcctctc gtgcagtatt ccattgaaca tccgattgaa actaatgaag
    18841 ccaatgtcgg aggcactcta aatgttttga ccgcggcgaa agaggcgggg gttaaaaaag
    18901 tggtttattc agcttccagc gccgcttacg gcgaccaggc agtaatgcct ctggttgaag
    18961 agatgccggc taaacccaag agcccatatg gtttgcaaaa atatatcggc gaaatgtatt
    19021 gtcggatttt ctcagaaatt tataatctgc cgactgtctg tcttcgctac ttcaatgttt
    19081 acggtccacg tcagaatgaa gaaggcgctt acgcgctggt tattggcaag ttcttgaaac
    19141 aaaaacgcga aggtctccct ttaacaatca ctggtgacgg cacgcagact agagattttg
    19201 tcagtgttca tgacgtggcg cgggccaaca ttttggcgat ggaaaaagag gttggcgggg
    19261 gtgaagtgtt caatattggc gcgggccaaa attacagcgt gaacgaggtt gcgaaaatga
    19321 ttggcggtga agtggagtat atcccagctc gcctagagcc ctatgtttcg caggcggaca
    19381 atcgcaaggc gcgcgaggtg ttaggttgga cgccaaccgt ctcgctcgcc gagggtttga
    19441 aagagttaac aagctaacaa aattcaagat gattattgat gggcggcaaa tggccgaaga
    19501 aataaaaaaa gagttaaaag aaaaactggc aaataaaaat cttcgtttgg tgatagtctc
    19561 agtgggggac aacgccgtat cagccaaata cttggcgcgt aaacaaaaat ttggtgaggc
    19621 ggtgggggta gaaacaattg ttcacgaatt tgccggtgat gtttctgaag atgaacttgc
    19681 tcggcacttg gcaaaattag ctgatgaccg ttcggttaac ggagttgttg ttcaattgcc
    19741 cttgccggaa cagttggaca cggaaaaatt attgactctt attccgtccg aaaaagatat
    19801 tgacgctcta tcgccggagg cgaaagtctt gccgccagtg gtcgcggcca taaaagaaat
    19861 tttagagaga aacagcattt tggttgaagg caaaaaggcg gtcgtgattg gtcgcgggcg
    19921 gttggtgggg cgaccggtgg cgttgtggct cgcgcagaat ggagctgagg ttgaggttgt
    19981 aactttagaa accaaaaatc caatggcggt tattgctcaa gctgacttgc tggtcagcgg
    20041 cgccggtcaa ccgggtttgc ttaaaccgga aatgctaaag gagggagtag ttttaattga
    20101 tgccgcgacg agcgaggtta ataataaatt ggcaggagac gccgaggcag cttgcgccga
    20161 taaatgctcg ctttttaccc cggtgcccgg cggagtgggg ccattgacag tcgcgtgttt
    20221 gttcaaaaac ctagcggcct taagtcaata aataataaag accgtttttg ttttcgtgtt
    20281 agagttttgt ttttgagtgg tttgtttgtt taacccagcc tcttttctaa aattgggcaa
    20341 aattaacaga gcttttcccg gttggacttt tttgaaattc tgcttataat ggattaaata
    20401 tgaataaaac acgtttaacc gctatactcc ttattttggc gggcctaggt ttaggttttt
    20461 ttgtttacta ttcggaaatc cccaatacgt ctttttaccg gccatttaag cttggtctag
    20521 acctttcagg tggaagtcgc ctgacttacg tggcggatgt ttcgcaaatt gacccatcca
    20581 atatttccga cgcgatgtct tctttgcgtg aagtaattga ccgtcggatt aacgtgtttg
    20641 gtgtttctga acccgtggtt caggtggagc gttcggcgat tggtggaata gaccaaaacc
    20701 gtttgaccgt ggaattgcct ggcgtgactg aggtggagga ggccgttaag atgatcaatg
    20761 agactccaac tttggaattt cgcactgaac gggcaacaac cagtccacaa agacaggcta
    20821 taattgcagc tcgcgaagaa attcaaaatt atattcttga tttgcccacg acaacgccgg
    20881 cagctttgat tgaagagatg ttggaagaga agtttgggca agagaagcta aatttggtgc
    20941 aagaggaccc ggattttgaa ccaaccgaat tgacgggccg ttatttggaa aaagcgcaag
    21001 tggtttttaa ccaaaattct ttggggccgt ctattttggt tgaatttgac cgagagggga
    21061 gtaaaatttt tgcagatttg accaaaaaca atgtcggcaa agccgtgggt attttcttgg
    21121 atggggcctt gatttcagct ccgattgtgc gtgaagaaat cagcgatggt caggccgaaa
    21181 tcagcggcca gttcactgtt ccagaagcta aaactttaac tcgcaatttg aatttaggcg
    21241 ccttgccggt gccgataacc ctggcctcca cacaggtggt gggcgcgacc ttgggcgagg
    21301 aagcgattca aaaaggggtg aaagccggag tgctcgggct tttgattgtt gccgtcttta
    21361 tgattctctg gtatcgcttg ccggggctgg tttcggtttt ggcattaatt ttttatatca
    21421 ttctgatgct ctcgctgttc aaacttatgc cggtgacttt gacggcggcc ggtatggccg
    21481 gtcttattct gtccattggt atcgcaattg atgctaacat tttgattttt gaacgaatga
    21541 aagaagaaat cgcggccggc aagggtttgc ttgaatcaat caaagaaggc ttctcgcgcg
    21601 cctggttgtc catccgtgat tccaacttgt ccagtttgat ttcagccgtc attttgtttt
    21661 ggtttggcag cagcttgatt aagggttttg ctttaacctt gatgattggc attttggtta
    21721 gtatgtttac cgcgattact gtcaccagaa ccttactttt ggcgcttggc tttcgtaacc
    21781 gggtcggctt ggccaagttt ttgtttgatt caggtttttc caaatagggt cgcgacccta
    21841 aaagaaataa atctaggcca ataaaaaaga aaaaatgttt atccaatctc tatcatctaa
    21901 tatctaacct atcatttatg ttaatcgtta aatacagaaa aatattttac actctatcac
    21961 ttgttttgtt ggcattgtcg gtttttgcga ttgctcgtta cggtttaaat ttgggcaccg
    22021 attttatcgg cggcactttg ctggaggtta aatatgaaga ggggagaccg gaattggaag
    22081 aattaaattc tgctgtctta gaccttgaat tgggtagtat tttaattcaa ccaacaggcg
    22141 aacaaaacta taccttaaaa ctaaggaata tttctgagac agaaaaagaa gccctttttg
    22201 aggccctcac cttgggtggt aaaaaggcat ttacccaaga gaggttaagc gaggttggcc
    22261 cgactttggg caaggagctc cgcaacaaag gcgtggtggc tatcaccttg gttgtgcttt
    22321 tgattatcct ctatattgct tttgttttcc gtcaagtttc caaacatcac gtaaaatctt
    22381 ggaagtatgg ggtggcggct atcgttgcct tgactcacga tatcattatt tcggcggggg
    22441 cgatggcctt gctcgggcat ttttccgggg ctgaagctga cgcgcttttt ttgacggcat
    22501 tattgacaat tttgggccta tcggttaacg acacgattgt tgtttttgac cggattcgag
    22561 aaaatcttca tcgccaccca acggttaaat ttgaggaagt ggtgggt
//
LOCUS       DAOOLO010000005        22046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_2292_length_22046_cov_7.320222, whole genome shotgun sequence.
ACCESSION   DAOOLO010000005 DAOOLO010000000
VERSION     DAOOLO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 22046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22046
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2292_length_22046_cov_7.320222"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1952
                     /gene="rny"
                     /locus_tag="PLH96_00570"
     CDS             423..1952
                     /gene="rny"
                     /locus_tag="PLH96_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPB60303.1"
                     /translation="MNPVTYILIASAIGVGGIVVGYFLKVLLTLAQKGSMENKIKQSF
                     LDAKDQAQKIVSDAETTADKIITEAKSKLEEKESQLKKTEDRLVKKDELLDKRQMDID
                     KEGDDLKKQAEEIKRTKEKLDEAEKEKIKELQKIAGLSLEEAKEELMTNIEKQYEEDL
                     LVRIKKLENEAGEKYEQKSKEILSTVIQRLSTSTVPEVMSTTVDIPSDDIKGKIIGKE
                     GRNIRAFEKATGVEVIIDDTPGSIVLSSFDPVRRQIAKTALENLIADGRIQPAKIEEI
                     VEKAKEEVSKVIKEKGEQAAYEVGVLNLDPRILLILGRLHFRTSYGQNVLQHAIEVAH
                     LAGMLAEELKSDPQVAKAGGLLHDIGKAVDHEIPGTHVEIGRRILEKFGADHKVIQAM
                     QSHHEEYPFETLESIIVKVADAISSSRPGARRDSLENYLKRLKDLEDIANSFQGIEKT
                     YAIQAGREIRVFVQPEDVNDLESKRLARDIALRIEQELKYPGEIKITVIRETRVIDYA
                     R"
     gene            2067..2339
                     /locus_tag="PLH96_00575"
     CDS             2067..2339
                     /locus_tag="PLH96_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB60304.1"
                     /translation="MNKQALVDVVHEVIGGTKAGAEKVVDVLIDSIISTLKKGEDVSI
                     AGLGIFTVKERAARTARNPRTGETVQVPAMSVPKFKAAKALKDAVK"
     gene            complement(2410..3237)
                     /locus_tag="PLH96_00580"
     CDS             complement(2410..3237)
                     /locus_tag="PLH96_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPB60305.1"
                     /translation="MKKYIYLNDRIVSLDKAAIFANDLGLLRGFGVMDFLRTFNGKLF
                     HLPDHYKRFCSSAKMLGLRVPVKEEELAKILSHLLAKNKVRDASFRLILTPGRTDNGL
                     DHNGLQTFFILTEDPYNLPDKMFIAGASLITSEYERILPESKHLNYLWAVKMYPVRKK
                     KEAVDLLYLKDGKVTECATSNIFIVKKDRLITPKKGILPGITRKVVLDLSKKLLQVKE
                     SEVTERELWLADEVFITATSKSVLPITKIDGKKIGGGQPGPWTKKIMTVFDEYKKNY"
     gene            3314..3384
                     /locus_tag="PLH96_00585"
     tRNA            3314..3384
                     /locus_tag="PLH96_00585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3346..3348,aa:Gly,seq:tcc)
     gene            complement(3841..4107)
                     /locus_tag="PLH96_00590"
     CDS             complement(3841..4107)
                     /locus_tag="PLH96_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPB60306.1"
                     /translation="MASKLYVGNLSYKTNDGELQQLFSQAGTVASARVIMDKMTGRSR
                     GFGFVEMETKEDADKAIDMFNGQEIEGRKLVVNEARPMEERPRQ"
     gene            complement(4291..5250)
                     /locus_tag="PLH96_00595"
     CDS             complement(4291..5250)
                     /locus_tag="PLH96_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60307.1"
                     /translation="MDNMETAAQAVPVPEGPKLLMGVWALLEKTWSIYKDKLWLAIGI
                     LVTPVVLMTLASYVGQTGLLLIASVISLVAALIMFWAQGALVLLFLNRESDLSVKSAY
                     KNAWPKYWSLLWVSILTTFITLGGFGLFVIPGLIFTVWFMFSMIVLMAEGERGIKALV
                     KSREYVRNNFWPVVGRYLVIMILVLLAYIILGIITAGLTDSNIWSVILANLLMLFVIP
                     ALVIYQVLLYEDLKAAKGVVEVKEQAKTTYLLIGAAGWAFVIILALLATSLVMSVLGF
                     ILGGGLSNDEDLSGGLETPEIQINLPDGVQERLEQLISEEVVS"
     gene            5446..5754
                     /locus_tag="PLH96_00600"
     CDS             5446..5754
                     /locus_tag="PLH96_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60308.1"
                     /translation="MATTDNKEGRLRIAIEAQVEELETHRAKLEEWAGRKLVTKGELM
                     AAWVGSGMAAAFREDFEKSVSDEVNRWHAGIRVQRPPSEADAWSQYRDHGGHLCHHDQ
                     "
     gene            complement(5880..6389)
                     /locus_tag="PLH96_00605"
     CDS             complement(5880..6389)
                     /locus_tag="PLH96_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60309.1"
                     /translation="MEKIVIVNDKMQRRYRYRLVEPAGKNFASDFKPELSPKEMLRLG
                     VFGGKYVTDCQKEFPADWFAKAKLSPEKHDARLNYFKVRASQPLAVWRARGWINNEHD
                     PRGWFQWYCRYWQGRRLPEEDARQISRWKKMRRHVTQVKKNCRAGDIFCRPRQRQALL
                     HWAYDSRSI"
     gene            complement(6392..6790)
                     /locus_tag="PLH96_00610"
     CDS             complement(6392..6790)
                     /locus_tag="PLH96_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB60310.1"
                     /translation="MRGFFNYNIERETIGNSDFRRVLYTGKHSQLVLMSLKPEEDIGL
                     EVHGNTDQFFRFEEGNGVVTIDGKNYDVKDGSVVIIPAGAEHNVKNTGRGDLKLYTIY
                     SPAEHRDKVVRKTKEEALANEEHFDGQTTE"
     assembly_gap    7295..7394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7395..7839)
                     /locus_tag="PLH96_00615"
     CDS             complement(<7395..7839)
                     /locus_tag="PLH96_00615"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPB60311.1"
                     /translation="MDLILSWFNFLPAEVLTFAVAATPISELRGAIPLALGVFGFSPV
                     KSFVIAWVGNLLPAVFLLWWLGPVSDFLSRHFVLAKKFFDWLFARTRRKFSGRYETLG
                     EVALVIFVAVPFPLTGVWTGAVAAFLFGLPKKRSLGLVGLGAAFAG"
     gene            complement(7941..8693)
                     /locus_tag="PLH96_00620"
     CDS             complement(7941..8693)
                     /locus_tag="PLH96_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPB60312.1"
                     /translation="MPTFLIYLVIFVAVIIISSIRQVNQYERGVKFTLGKFSSVMEPG
                     WRLVIPVFQFYRKVDMRVKAVDVPDQNAITRDNVSVAVNAVIYYKVASAEKAVIEVED
                     FYYAISQYAQTTMRNIVGEVTLDELLAGRETIANRIKEIVDKETDEWGLKVNNVELKD
                     IRLPENMERTIAKQAEAEREKRAVIITSEGELAAAENMAQAAKILSSADGALHLRTLQ
                     SINDLSSDQSNTVVFTIPLDILKAISGFKKEN"
     gene            8888..9169
                     /locus_tag="PLH96_00625"
     CDS             8888..9169
                     /locus_tag="PLH96_00625"
                     /inference="COORDINATES: protein motif:HMM:NF039180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HPB60313.1"
                     /translation="METTFITESFGSWLGTLPAQVWGIASITAFFGLTILMVVLIVWS
                     ITWKGLALWKAAKLNQKVWFIILLVVNTAGILEIIFYFLVARPKEKLGQ"
     gene            complement(9217..9765)
                     /locus_tag="PLH96_00630"
     CDS             complement(9217..9765)
                     /locus_tag="PLH96_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60314.1"
                     /translation="MNKEMTGDEVISRGAHRQAIKTGLPDELIITSHDVGCCAFQDAL
                     FCSMLLEALDRGGVPTPYIEFSDRGGVRVFVAKKLGQVEVSAEMGGLTGDEICVIAKN
                     CLKSEFGSEIDNLAKSAFASLRDFFKTKGLMLVAMKFSLGVGQTVLSDGERLLVFGLI
                     GPEDIVVLDDSSREVKNPQFLF"
     gene            complement(9897..10610)
                     /locus_tag="PLH96_00635"
     CDS             complement(9897..10610)
                     /locus_tag="PLH96_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60315.1"
                     /translation="MKKTVLMLTLALVLTLGLTVSVKAVVSEEFGGNKSNQNESKPEQ
                     ETNQNENKQKSETTVTTMPELERKGENNNNDQDKPLAPELWTDTMEIAVDGGSLPPVK
                     VSAENVSIQGNKLLFFSSSTGSVNEYQMKTALPAFVNRLVKAWRKEGATTTIENFEIR
                     IEEDKPIYEVSVMETGKLLGFIPLKLERKAVISATTEEIEEVKQPWYSFLVRKLNLFK
                     IQNEGKGENATTTDQNPVE"
     gene            10861..10933
                     /locus_tag="PLH96_00640"
     tRNA            10861..10933
                     /locus_tag="PLH96_00640"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10894..10896,aa:Pro,seq:cgg)
     gene            11005..11220
                     /locus_tag="PLH96_00645"
     CDS             11005..11220
                     /locus_tag="PLH96_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60316.1"
                     /translation="MNKKISLLYLVKNLSIVVGLVLVWRGVWYVLDEIDLLFFGDNHF
                     WTALTGIILGLIILYLPDKNLKEIEKL"
     gene            11273..11346
                     /locus_tag="PLH96_00650"
     tRNA            11273..11346
                     /locus_tag="PLH96_00650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11305..11307,aa:Pro,seq:ggg)
     gene            complement(11460..11532)
                     /locus_tag="PLH96_00655"
     tRNA            complement(11460..11532)
                     /locus_tag="PLH96_00655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11497..11499),aa:Pro,seq:tgg)
     gene            11689..11997
                     /locus_tag="PLH96_00660"
     CDS             11689..11997
                     /locus_tag="PLH96_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60317.1"
                     /translation="MDIQLFLCPKGGAMLEYNVVFECDDGIRTWRSYSSREEFENQQA
                     QTTQKVVAKGISSQKCVSLCRDVSPSFILAHKKDRAVEPFGHLALQVELMNIIFIQCH
                     "
     gene            complement(12090..12521)
                     /locus_tag="PLH96_00665"
     CDS             complement(12090..12521)
                     /locus_tag="PLH96_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPB60318.1"
                     /translation="MFDFLKFVKRRGETEKERIGRLGEDEATRWLKKEGFKIIERNYW
                     KPWGEIDIIAQKGQALHFVEVKTVCLERGELARFDRYEPEDNIHPWKLKRLSKAVESY
                     LLEKYGDKDEEDPDWQMDALCLYLEKGSEKVLKIEYLEEIG"
     gene            13092..13163
                     /locus_tag="PLH96_00670"
     tRNA            13092..13163
                     /locus_tag="PLH96_00670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13125..13127,aa:Arg,seq:tct)
     gene            complement(13304..13612)
                     /locus_tag="PLH96_00675"
     CDS             complement(13304..13612)
                     /locus_tag="PLH96_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60319.1"
                     /translation="MKKLWLPKIKINFSEGAFEKVFNPFAFWKMIISLFFVGFILVTV
                     LHLLLFYWFESKVEEEESAQMTESLVDRDRLSRAYRQIKLKEENFNKYFDEKPAENAD
                     "
     gene            complement(13614..14114)
                     /locus_tag="PLH96_00680"
     CDS             complement(13614..14114)
                     /locus_tag="PLH96_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60320.1"
                     /translation="MKKEKNIFYIMLAVCLVCLLLWSFLFWLIWQAQKTPEKVEASAE
                     TLFSESDLESLRRFWGSLETQLAAVEGQFIDKDMTVDFIEEIESVARQTDVALKVTGA
                     QDSPDIVVSFTGEGNFRNLLQFQLLLENLPHKLFFNKFSIYKKSATDGGENWLMDVSV
                     KLESIK"
     gene            complement(14116..14670)
                     /locus_tag="PLH96_00685"
     CDS             complement(14116..14670)
                     /locus_tag="PLH96_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60321.1"
                     /translation="MFNLLPEKNKKENRRRFIYRRLVVVTTMISGLIFVACVFLAAVY
                     LHLSFGGIGEKEKEKSLAELSPADQSLVKEIQTLNKKLEIILPVEDAILPSEVVLAAV
                     NDKGEGISLHHLALECQAQTKICNVAALGRAGDRQSLLDYVAKLKQNKMFKSVESPIN
                     NLINDKDSQFSLELETNLVKDLLD"
     gene            complement(14678..15772)
                     /gene="pilM"
                     /locus_tag="PLH96_00690"
     CDS             complement(14678..15772)
                     /gene="pilM"
                     /locus_tag="PLH96_00690"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPB60322.1"
                     /translation="MKSKIFFEFFPVPDYLLVPAAGLDISDRSVKYVELSHNGQIKVK
                     RYGEKPMPAGVIEEGRIKNKTEAIKVLSELKKEMGLNYIFASLPEDEAFIVRMKMPPM
                     KKSELRGSIELQLEEYVPLPPDQCVFDFEMISSAREVEKNGAEVSVSVLPKAIVDDYL
                     EIFTAAGLEPLLFEIEAQAIARALVKEGDPGTSMMVDIGKIHTGFSVVQADQVVFTSI
                     IGLGGSNITFCLQKALGLDAVAANKLKEEKGLLRSQENQEVFSALMPPVSALKDEIKQ
                     RFSYWQNHYDKDEGKRSSIDRVILVGGQSTLPGLREYLETHLEVPVVLGNPWVNFNLK
                     QDELPVIDYNNAMRYVTALGLALKNSHLKR"
     gene            complement(15854..16111)
                     /locus_tag="PLH96_00695"
     CDS             complement(15854..16111)
                     /locus_tag="PLH96_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60323.1"
                     /translation="MNKGLQEKIAKTVDELFSLMGAAFMALLWSLGEIGLLVKGAPFR
                     AVSLLPIAILVLLVFVLARRARVLSQLKLEARKDFERRDLG"
     gene            complement(16201..16605)
                     /locus_tag="PLH96_00700"
     CDS             complement(16201..16605)
                     /locus_tag="PLH96_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HPB60324.1"
                     /translation="MRLLGIDYGHKKVGLALSDEGGRFAFPHNTWPNDKFLVGKIKEL
                     CAREKVGLIVVGESLDLSGRPNPLMREIEKFEDLLKQKIGLPLAREREFFSSWEAKRI
                     IDEEQTDPLTDARAAALILKSYIDRQGNAEVV"
     gene            complement(16608..17189)
                     /locus_tag="PLH96_00705"
     CDS             complement(16608..17189)
                     /locus_tag="PLH96_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60325.1"
                     /translation="MRKKILISLTAAILLLVVGLALRFCPCLEPSSKDLVWAKQISKI
                     KTILKETEDISAEGQLRPVQVVREADLTGDGVPEALVAIGSGGAYTDDLALFIWQGDK
                     PVLAKFIDAAGEEGPIILSEGASVRNGASVNWKPEQKVIYAGSWNVDFDGALADCEVN
                     AYQYNRDSLAFVYNQNLSADLGSQFCADLRQSL"
     gene            complement(17300..17734)
                     /locus_tag="PLH96_00710"
     CDS             complement(17300..17734)
                     /locus_tag="PLH96_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60326.1"
                     /translation="MGKKDKEKEKESPHCLNCSASLVPAEETACVVALKHLFEKWGLV
                     ARLEDLHLAVVIDTLFEEELRYRCYVFLAKQWQYADDAKRKINQDCWNRLVSLPEEVW
                     SSDHSWFLEVPRSCLKAQVIYDADGSQLRSFGLQQLGSALAG"
     gene            complement(18002..18931)
                     /locus_tag="PLH96_00715"
     CDS             complement(18002..18931)
                     /locus_tag="PLH96_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HPB60327.1"
                     /translation="MLETLLIFAFSLFLVVKGATLATKYSAKLAEHFSLSRYVIGFVV
                     VAFISILPETIISINSALQGIPEFGLGTLFGSNIADLSLIFALLILLSGRSIKIESKI
                     LKNVDLYPLFLMFPIILGLNGHFSRIDGAILVITGLIFYYFVFRNGTEASKVFSNRNG
                     KYKNILFLLSAMAMLLIGAHFTVRSATALAEMLNITPILVGMLVVSLGTTMPEFLYSF
                     KAVRQKKDSLAIGDILGSVLADATIVIGVLALISPFYFPARIVYVTGTFMVVASIFLL
                     RFMRSGRTVTKKEGAMLLLFWLAYVVVEFFVSK"
     gene            complement(19284..19457)
                     /locus_tag="PLH96_00720"
     CDS             complement(19284..19457)
                     /locus_tag="PLH96_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60328.1"
                     /translation="MAKKIDINTRFYIDLDIKTKKIIGWDYGQRQGLEQKLSNPNHQR
                     IFILPGMRQKIRV"
     gene            complement(19441..20235)
                     /locus_tag="PLH96_00725"
     CDS             complement(19441..20235)
                     /locus_tag="PLH96_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPB60329.1"
                     /translation="MNTKLLKISTLGAYIVMVVVNALAVWLPIAGRETGEISDSYPNL
                     FAPAGYAFSIWGLIYTLLAIYVIYQLGQGKNELVARINRLFIVNALLNAAWIFAWHYD
                     VIWLSVLLMAGLLYTLIRLANILRVGNLGQKEKSLARLPFSVYFGWITVATIANITVF
                     LVSIGWNRFGLSEVFWTIVILLVGALIGSWRMLYDRAIAYGLVLIWAYAAILYKHLAE
                     SGFAGEYPTVIGAAILCLAVFAGMIVFIGIKKKTNNPTTTNYGEEN"
     gene            complement(20273..21214)
                     /locus_tag="PLH96_00730"
     CDS             complement(20273..21214)
                     /locus_tag="PLH96_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60330.1"
                     /translation="MSTIQKILTGVVAVAILGVSAYLLLNKFGDSQPQNPLDNPEKIV
                     SAYESLLIEAEQAKTANADCGDKKGLAKQIKSLEDGLADLAERKKDWLDNVPQLPEGG
                     PTSVTPEGRPGSEVPELTSDVPPLPELGPDVIVVPGSKVPELTSDAPSLPDINIQVVD
                     DLGRPVEYVPELPELNTGRPGSEVPTLDSNVPPLPEINIADSNEPIIKMAELEQKIKN
                     LLQELKTLCPEEENKKVISDKCSDACQRHKDCAAYTEDVTAADLTDAYNTCMEECPTW
                     PKEMIKCINAVDIKTPNDCVSFVQCQVPQLYEERYLE"
     gene            complement(21251..21697)
                     /locus_tag="PLH96_00735"
     CDS             complement(21251..21697)
                     /locus_tag="PLH96_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPB60331.1"
                     /translation="MKHIRLENSWLVKAPLGEVFNIITDFENLPKNFPKVAKSLVITK
                     REGNHLEIDAQVKTFGQTFPVKMKTSIIPERGFISDNESPKFGTSGHEELLLEKVEGG
                     TKINYVYEVDIHKKWLRVIAKPLIGWFAMKAWEKAVIEELRKILGR"
     gene            complement(21809..21982)
                     /locus_tag="PLH96_00740"
     CDS             complement(21809..21982)
                     /locus_tag="PLH96_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPB60332.1"
                     /translation="MRKLADKNTRKLQKLGKTSLAVTIPKEIITELKWREKQRVKIIR
                     IRGGFVVRDYRSK"
BASE COUNT         6530 a         5326 c         4166 g         5924 t
ORIGIN      
        1 aagtagttct gatacagaac gcacgcagat tctcgcaaat gaaatgtcga gaaaaaagca
       61 attaacgatt gatagttaat ctgaaataag aaagttcaaa caccaaaggg cccgaccttg
      121 ttagttgcta acaaggtcgg gcccttttta ttatttccct gaaaactgaa ctttatctgc
      181 ataaatccgc attttgtctg caagaatctg cgtctaatcc gcatcagttg gacaaacttc
      241 attctaggtg atatcatcaa agtaccaatt atttaatggt tgtaaattta tcaaaaataa
      301 catttaccgc taaaatcttt ttgtccgtaa cgtaaaaata tcttcattat gttgattttg
      361 ccaagaaaaa taactccaaa aatcacgcat aacggctaaa gaccaggcgg ttcgtattga
      421 ttatgaaccc agtaacatac attctcatcg cttcggccat cggagtcggc ggaattgttg
      481 ttggctattt tttgaaggtc ttgttgaccc tggcccaaaa aggctccatg gaaaacaaga
      541 ttaagcagtc cttcctggat gccaaggacc aggcccagaa aatagtcagc gacgcggaaa
      601 caacggccga caaaataatc accgaggcca aaagcaagct agaagaaaaa gaaagtcagt
      661 tgaaaaaaac cgaagaccgt ctcgttaaaa aagacgagct tttggataaa cgccagatgg
      721 atatagacaa agaaggcgat gacttaaaaa aacaagcgga agaaattaaa cgaaccaaag
      781 aaaaactaga tgaggccgaa aaagaaaaaa tcaaagaact acaaaaaatt gccggccttt
      841 ccctggaaga agcaaaggaa gaactaatga ctaacattga aaaacaatac gaggaagatt
      901 tgttggttcg tatcaaaaaa ctagaaaacg aagctggtga aaaatacgaa caaaagtcca
      961 aagagattct ttccacagtg attcaacgtt tatcgacttc caccgtgcca gaagttatgt
     1021 ccaccacagt agatatcccc tcagacgata taaagggcaa gattatcggc aaggaaggtc
     1081 gcaacattcg cgcctttgaa aaagcgactg gggttgaagt tattatagac gacacacccg
     1141 gctctatcgt tttgtcttca tttgacccag ttcgtcggca aatcgccaaa actgccttgg
     1201 aaaatttaat tgccgacggc cggattcagc cagctaaaat agaagaaata gtggaaaaag
     1261 cgaaggagga ggtttccaag gttatcaaag aaaaaggcga gcaagccgct tatgaagtcg
     1321 gggttctaaa cctggacccg cgtattcttc tcatccttgg tcgtctgcat tttcgcacca
     1381 gctatggcca aaatgtgctc cagcacgcga ttgaagtagc tcatctagcc ggtatgctcg
     1441 ctgaagaatt gaagagtgac ccacaagtgg ctaaggcggg cggtctcttg catgatattg
     1501 gcaaggccgt tgaccacgaa atccccggga cgcacgtgga aatcggtcgc cgcattctgg
     1561 aaaaattcgg cgccgaccat aaagttatcc aagcgatgca atcccaccat gaggaatatc
     1621 cgtttgagac cctggagtcc atcattgtca aagttgccga cgctatttcc agctccagac
     1681 cgggcgccag gcgtgacagt ttggaaaatt acctaaaacg cttgaaagat ttggaagata
     1741 ttgccaactc tttccaaggc attgaaaaaa cctatgccat ccaagcaggc cgagaaataa
     1801 gggtttttgt tcaaccagag gatgttaacg acctagaatc aaagcgcctg gcgcgagaca
     1861 ttgctctccg tatagaacaa gaactgaaat acccgggcga aattaaaatc accgtcatcc
     1921 gcgagacaag agtcatcgac tacgccagat aatttgactg gtgtcaaaag gggtttcaac
     1981 tattgcttta aaatctcctt gtattataat gtttaatagc atttattaag ctaaggtcgt
     2041 ctaattagca ataagaaata actattatga ataagcaagc tctcgtggat gtagtacatg
     2101 aggtaatcgg cggaaccaaa gccggtgccg aaaaagtcgt agatgtgttg attgattcca
     2161 tcatctccac cctaaagaag ggggaagatg tctctatcgc cggtttgggt attttcaccg
     2221 tcaaggaacg cgcggctaga accgcccgca atcctcgcac cggcgaaacc gttcaggtcc
     2281 cggctatgag cgtaccgaaa ttcaaagccg ccaaagcttt gaaagacgcg gtcaaataaa
     2341 aaactaggga taacatcatt tctgaactaa atataaaacc ccgagcagaa ctcggggttt
     2401 tatatttagt taatagtttt tcttgtattc gtcaaaaact gtcattattt tttttgtcca
     2461 aggaccaggt tgaccacctc caattttttt gccgtcaatc ttagttattg gcaaaacact
     2521 cttacttgtc gccgtaataa atacttcatc ggccaaccac aattctctct cggttacttc
     2581 agactcttta acttgtaaaa gtttcttaga taaatcaaga acaacttttc tggttatgcc
     2641 aggcaaaatg ccttttttag gagttatcaa tctgtctttt ttaacaatga agatattgct
     2701 agttgcacat tcagtaactt taccatcttt taaataaagc aggtcgacgg cctctttctt
     2761 tttgcgaact gggtacattt ttaccgccca aagataattc agatgtttag attctggtaa
     2821 aattctttcg tattctgagg taatcaaaga agcgccggca ataaacatct tatcgggtaa
     2881 attatatgga tcttcggtca aaataaagaa agtttgtaaa ccattatgat ctaaaccatt
     2941 gtcagttcga ccaggtgtta aaattaaccg aaaactagca tctctaactt tattcttagc
     3001 caataaatga ctgagaattt ttgctagttc ttcttcttta accggtactc ttaaacctag
     3061 cattttggct gaactacaaa aacgtttgta atgatctggc aaatggaata gcttcccatt
     3121 aaatgttcgc aaaaaatcca ttaccccaaa accacgcaaa agaccgaggt cattcgcaaa
     3181 gatagccgcc ttgtcaagcg agacaatacg atcattaaga taaatatatt ttttcatctt
     3241 tacattttac tttacgttta ttagcgccac aacattgaac ctaacccaat tttctgttag
     3301 tattttaacg tttgcgggca tggtttagtg gtagaatgac tgccttccaa gcagtagacg
     3361 ggagttcgat tctccctgcc cgcacccaca caataaaagt taatcacctg gttaagccgg
     3421 tattaatgtc ggtgcttgcg caaaagaatg agaattaata ccaaagacaa aattgctggt
     3481 attgaacgcc aaatcttcgc cttgtcaaaa gtttcaaaaa ctaaaaccaa aaaaccaagc
     3541 gccaaaaaac gcaacgtcca agattgaaga ctcctctgtc ctcggcgcgc aaaccaccac
     3601 tgaagaagcc aggcgccggc gataatactc aaaccataaa acatacctta aaagcaaata
     3661 ttccctgatg ctcagccttt attataaatt atttttgctc aaaaacgaaa ttttggaaaa
     3721 tattttatgg ggcttccaaa tcgtactcaa aataaaacca gcaaaaaagt tgaacaaaaa
     3781 tcgccccact cctctgacga gggtggggcg attttatcac ggcctaaagc cgctttttgt
     3841 ttattgtctc gggcgctctt ccatcggtcg agcttcgttg accaccagct tacgtccttc
     3901 gatctcctga ccgttgaaca tatcaatcgc tttatcggcg tcctccttgg tttccatttc
     3961 tacgaaacca aaaccacggg atcgtccagt cattttgtcc ataataacac gagcggaagc
     4021 gaccgttccc gcctgtgaga agagctgttg gagctcgccg tcatttgttt tgtacgacaa
     4081 gttgccaacg tataacttgc tagccatcta acttaaatta actacttact aatttggagt
     4141 aaggtgacct tcctaagcta gacccaagat ctaattaaga aacttacttc gtcttcatta
     4201 aaacaaaaag acttctataa gtcaaaccga gcaagccttc accacctcac caaaatggcc
     4261 cgggaactcc gggccatcgg agggcgactt ttacgaaacc acttcttcgg agatcaattg
     4321 ctctaaccgt tcttgaactc cgtccgggag gtttatttga atttccggcg tttccaaccc
     4381 tccagacaaa tcttcatcat ttgagagacc gcctccgagg ataaagccca ggaccgacat
     4441 caccaaggaa gtcgccagca gggccaaaat aatgacaaaa gcccaaccgg cagcgccaat
     4501 caaaagatag gtggttttgg cttgttcttt tacttccact actcccttcg ccgctttaag
     4561 atcctcataa agcaagacct gataaataac gagggccgga ataacaaaca acatcagcaa
     4621 attggctagg atcacggacc agatgttgct gtctgtcaac cccgccgtga tgatacccag
     4681 gatgatatag gccaacaaca ccaagatcat gatgactaaa taacgaccaa ccaccggcca
     4741 aaaattgttc cggacatatt cacggctttt aaccaaagct tttattcctc tttccccttc
     4801 cgccatcaaa acaatcatcg aaaacataaa ccaaacagta aagatgagac caggaataac
     4861 aaagagccca aaaccgccaa gcgtgataaa ggtcgttaga atagataccc aaagcaaaga
     4921 ccaatactta ggccaggcat ttttataagc gctttttacg ctcaaatctg actcgcgatt
     4981 aagaaagagg aggaccaaag cgccttgggc ccaaaacata atcagcgcgg caaccaaact
     5041 gataaccgac gcgatcagca acaaacctgt ctggccaaca taagaagcca gggtcatcaa
     5101 aacaaccggc gtcaccaaaa tacctatcgc caaccaaagt ttgtctttat aaatcgacca
     5161 ggtcttttcc aacaaagccc agacccccat tagcaatttg gggccttccg gcaccggcac
     5221 ggcttgagcc gctgtttcca tattatccat aagcttgtat tctcccaaag gaggttagct
     5281 gttaatttac ttttattctt ttattgtaac aaaactataa ttttactaaa gagcaagctt
     5341 gaatgattcc attgagaaac ataccacagt taagattata ttatttaaat gatataataa
     5401 taaacagaga ttttatgtct attgcactta aaaggaggtt gtcccatggc gacgactgac
     5461 aataaagaag gccggttgcg aattgcaatt gaggctcagg tagaagaact ggaaacgcat
     5521 cgcgctaagc ttgaagagtg ggccgggcgc aaactggtca ccaagggaga gctgatggcc
     5581 gcttgggtcg gaagcgggat ggccgcggct ttccgcgaag actttgaaaa aagcgtgtcg
     5641 gatgaagtga acagatggca tgccggcatc agagtccaaa ggccgccgag tgaggccgat
     5701 gcttggtcgc agtatcgaga ccatggtgga cacctttgtc accatgacca ataacaacgc
     5761 caggccgacc accaattaag ccggagtttc ctccggcttt tgttaatata aaaaattaaa
     5821 atttgaattg aaaccttttt gtccaatcgg gctaatttta ttaatgatac ttttttgaat
     5881 taaatcgacc gactgtcata ggcccaatgc aaaagcgctt ggcgttggcg cggccgacaa
     5941 aaaatatcac ccgctcggca attttttttg acttgcgtca catgtcgacg catctttttc
     6001 caacgactaa tttgccgagc atcttcctct ggcaaacgcc gaccttgcca ataccgacaa
     6061 taccactgaa accaaccgcg cgggtcatgt tcgttgttaa tccaacctct ggcgcgccaa
     6121 accgcaagcg gctggctagc gcgcacttta aaataattta accgagcatc gtgcttttca
     6181 ggactcagct tggctttagc aaaccaatcg gctggaaatt ctttctgaca atcggtcaca
     6241 tacttgcccc caaaaacgcc gagccggagc atctccttgg gcgagagctc cggtttgaaa
     6301 tcggacgcga aattttttcc agctggttcc actaagcgat accggtagcg acgttgcatt
     6361 ttgtcattaa cgatgacaat tttctccatg tttattcagt cgtctgacca tcaaaatgtt
     6421 cttcgtttgc caacgcttct tcttttgtct tccggaccac tttgtcccga tgttcggccg
     6481 gtgagtaaat agtatagagc ttcaaatcgc cgcgaccggt gttcttaaca ttgtgttcgg
     6541 cgccagctgg gataatcacc accgaaccat ccttgacgtc ataatttttt ccgtcaatgg
     6601 taacgacgcc attgccctct tcaaaacgaa aaaactggtc agtatttccg tgcacttcta
     6661 acccgatgtc ttcctctggt tttaaactca tcaaaaccaa ttgcgaatgt ttgccggtgt
     6721 aaagcacccg gcgaaaatca ctattgccga ttgtttctct ttcaatatta taattaaaaa
     6781 atcctctcat atttttattt aattaaccac taatagattt attataactc tttttctgaa
     6841 ttttctcagt tttttaaaat tccttgtttt aatttctgcc aaaataaaaa tccaaaaaac
     6901 agagagttcg ccgtcaacgc aaaccccaaa attgttgaag cgatttatga aactgaagct
     6961 gattatttta aaatttttta agtaggtaac cgtgtaacaa aatggctccc aaaaataacc
     7021 tgattttaca agcttttttg gcgaccccac aaatcaaatt gcccactgcg cccgcctgtg
     7081 tttcatcgcg aacacgaaaa tttttggcga aaaatatgat tctagatttt tcccgacaac
     7141 aagcagacgc gccacaacta aaatatttta agttttagga cgttgtatct gtattttaaa
     7201 ataactcaat aacccccagc gaaagagcgg tcactatcaa accggcaaac gctgcgccca
     7261 aaccgacaag tccaagcgaa cgtttcttgg gcaannnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnaaccgg caaacgctgc gcccaaaccg acaagtccaa gcgaacgttt
     7441 cttgggcaaa ccaaacagaa atgccgccac tgcgccggtc caaacgccgg tcaagggaaa
     7501 cgggaccgcg acaaaaataa caagcgcgac ttcccccaac gtttcataac gaccggaaaa
     7561 ttttcgcctt gtccgcgcga acaaccaatc aaaaaatttt ttcgccaaaa caaaatgacg
     7621 gctcaaaaaa tcagaaaccg gcccgagcca ccagagcaaa aacaccgccg ggagcaagtt
     7681 gccaacccaa gcaataacaa aacttttaac cggcgaaaaa ccaaacacgc caagcgccaa
     7741 aggaatggcg ccgcgcaatt ccgagatcgg cgtagccgcc acggcgaacg ttaaaacttc
     7801 agccggcaaa aaattaaacc aacttaaaat cagatccatt accaaccatt aaaacagaaa
     7861 tgaataaaaa aataaacaaa caaaaaaagt ttcggtttgg aaaccgaaac tttttttgtt
     7921 ttgggtattt actcagatat ttagttttct tttttgaagc cagaaatggc cttcaggata
     7981 tccaaaggaa tggtaaagac cacggtgtta gactggtcgg aagaaaggtc attgatcgat
     8041 tgcagggtgc gcaagtgaag cgccccatcg gcactcgaca aaattttagc cgcttgcgcc
     8101 atgttctccg ccgccgccaa ttcaccctct gaagtgataa taaccgctcg cttctctcgt
     8161 tctgcttcag cttgcttcgc gattgtgcgt tccatgtttt ccggcaagcg gatatctttg
     8221 agctcaacat tattaacctt cagcccccat tcatcagttt ctttatcaac aatctcttta
     8281 atccgattgg caatcgtttc tctccccgcg agcagttcgt ccaatgtcac ttcgccaaca
     8341 atgtttcgca tcgttgtctg cgcatattgc gagattgcat agtaaaaatc ttcaacttca
     8401 atgaccgctt tctcggccga agcgacttta taataaatca ccgcgttgac cgcgactgaa
     8461 acattatccc gagtgattgc gttctggtca ggcacatcta ccgcttttac tcgcatatcc
     8521 actttacgat aaaattggaa aaccggaata accagacgcc aacctggttc catgaccgaa
     8581 gagaatttgc caagagtaaa cttaactccc cgctcatatt ggttaacctg cctaatgcta
     8641 gagataataa tgaccgccac aaaaataact agatatatca aaaaagttgg cataaaaaat
     8701 aaattaactt ataactttct cagtataatt aaaacaaaat taagcgcaac aaaatttaag
     8761 aggcaaaaac atcaatattt attttgataa ctttagtttc taaattttga tttaatcaaa
     8821 caaacaatct gccacagaaa aaattctgtt tgccataaaa caagttaaat tttataataa
     8881 aaacattatg gaaaccacat ttatcactga gtcttttggc tcatggcttg gcactttgcc
     8941 agcccaggtt tggggcatcg cctcaatcac tgcctttttt ggtcttacca tcttgatggt
     9001 agtcttaatc gtttggtcga ttacttggaa aggtttagcc ctttggaaag cggctaaact
     9061 aaaccaaaaa gtttggttta tcatcttgct cgtagtaaac accgccggca ttctggaaat
     9121 tattttctat ttcctggttg cccgcccaaa agaaaagttg ggacagtaag attataaaaa
     9181 acagccccgc tcctctgacg agggcggggc tttcggttaa aaaagaaact gagggttctt
     9241 cacctcccga gagctatcgt caaggacaac aatgtcctcg ggcccaatga gaccaaagac
     9301 caggagtcgt tccccgtcag acaaaaccgt ctggccaacg ccgagactaa atttcatcgc
     9361 caccaacatc aacccctttg tcttgaaaaa atcgcgaagg gaagcgaagg cggattttgc
     9421 caagttgtct atctcggaac caaattcaga cttaagacaa ttcttggcga tgacgcaaat
     9481 ctcgtctccg gtcaagccac ccatctctgc cgagacttca acctgaccaa gtttcttggc
     9541 gacaaaaact ctgactcccc ctctgtcact gaactcaata tagggagtgg ggacaccgcc
     9601 ccgatccaag gcctccaaca acatgctaca aaaaagcgcg tcctggaaag cacaacatcc
     9661 aacgtcgtga gacgttataa tcaactcgtc cggcaaacct gtcttaatcg cttgccgatg
     9721 cgcgccccga ctaataacct catcgcccgt catctcttta ttcatttccc ctctctttct
     9781 taactttcaa tcagccgcca tagctgaaca tttccagagc tgttttagca ttttctttag
     9841 cccaagtcaa aacccgccct ctcaacaagg gcgggtctga aacttaaact tggttattac
     9901 tcaaccggat tttggtcagt cgtggtcgcg ttctcccctt ttccttcatt ctgaatctta
     9961 aataaattca gcttacgcac caagaaagaa taccagggtt gttttacttc ttctatctcc
    10021 tcggtggtcg ccgaaatgac cgcctttctt tctaacttaa gcgggatgaa accaagcaat
    10081 ttcccggtct ccatcaccga aacctcgtaa attggtttgt cttcttcaat tctgatttca
    10141 aaattttcaa tcgtcgtggt cgcgccctcc ttgcgccaag ccttgaccaa tcggttgaca
    10201 aaagccggca aggcggtttt catttgatat tcattaaccg aaccggtgct gctgctaaaa
    10261 aacaaaagtt tattgccttg aatactgaca ttttcggctg acacttttac tggtggcaaa
    10321 gaaccgccgt caaccgcaat ctccattgtg tcggtccaaa gttcgggcgc caatggtttg
    10381 tcttggtcgt tattattgtt ctcccctttt ctctccaatt ctggcatagt tgtcacggtc
    10441 gtttctgact tttgtttgtt ctcattttga ttggtttctt gttccggttt ggactcattt
    10501 tggttgctct tattgccccc aaactcctca ctgacaacag ccttgactga aacggtcagc
    10561 ccaagagtta aaaccaaagc caaagtcaac attaacactg tttttttcat ataggcgcta
    10621 aaaattatta ttactaaggc taataaaaat tacacctgaa ttttagcaaa gacgaacccc
    10681 tttttcaaga aaacgaatag gcagcccaaa agtgacaaat atttaatttt gaacacttct
    10741 gtgctaagat gaacgcggcc aagggaagcg agtgagcaac ctcgcccact taaagcccgc
    10801 gcaaagcagt ttggctcctt agaacgccaa caaggacaaa tatccaaaat tgaataataa
    10861 cgggatgtgg tgtaacggct aacatacacg attcgggttc gtgtgactcc gggttcgaat
    10921 cccggcatcc cgacattgcc tcaacaaagc catgatacat tcgagcacca accgagaccc
    10981 gaatgatgac aaagtttaaa ttatatgaat aaaaaaattt ctttacttta ccttgttaaa
    11041 aatctttcta ttgttgtcgg cctcgtgctt gtctggcgag gcgtctggta cgtgcttgat
    11101 gagatagacc ttctattttt tggtgataac cacttctgga cagctttgac cggaattatc
    11161 ctaggcttaa ttattcttta tttgccggac aagaacctca aagaaataga aaagctttag
    11221 cggttggtat ttccaaacaa ttgagttaag attaaaaaaa ccgataaaat atcgggccat
    11281 aggatagtgg tagtccttgc ccatggggtg ggtagagcgg cagttcgatt ctgcctggcc
    11341 cgaccaatta agactttttc caaatctaga aacattctcc ggtcaaaata aaagccgcgt
    11401 cgaaaaattt gagcttaaaa aaccaatcgg atagtctccg gtcagtcctt tcgcttcgtt
    11461 cgggccgccg ggattcgaac ccggagtcac ctgtacccaa aacaggcatg ttagccgtta
    11521 caccacggcc cgctaaatac catgccaata tacaccaatt tgtttaaaaa agtaagctgg
    11581 taattttttt cacaaaatta ttttgtcgct taattccatt cccgctcaac atcaaaagac
    11641 accgagcgtt tattttgacc ccttgttctt tttgccttat tattaaagat ggatattcag
    11701 ctttttctct gtccaaaagg aggagccatg cttgaataca atgtggtctt tgaatgtgat
    11761 gatggcataa gaacgtggcg gtcttattct tcaagggaag agtttgagaa tcaacaggcc
    11821 caaacaaccc aaaaagtcgt ggccaaagga atctccagcc aaaaatgcgt tagtctttgc
    11881 cgagatgttt ctccaagttt catcttggct cacaaaaaag accgggccgt tgaacctttt
    11941 ggccatctgg cattgcaggt ggagttaatg aacattatct tcattcaatg ccactagccc
    12001 attcccaccc ggcgcgctct agcgccgggt ggttttttat ttcgcccaaa accgacccac
    12061 tgcgtctcca ttgcttaggc aaacatttcc taacctatct cttccaaata ctctattttc
    12121 aacacttttt cactcccctt ttccagataa agacagagag cgtccatctg ccaatccgga
    12181 tcttcttcgt ccttatcacc atatttttct aaaagataag attccaccgc cttagacagg
    12241 cgttttaatt tccacggatg aatgttgtct tctggctcat atcggtcaaa gcgcgccagc
    12301 tctccccttt ccagacaaac tgttttgacc tccacaaaat gcagagcttg tcctttttgg
    12361 gcgataatat caatctcgcc ccacggtttc cagtaattgc gctcaattat tttaaagcct
    12421 tctttcttca accaccgcgt cgcttcgtct tcgccaagac gaccaattct ttctttttct
    12481 gtctcccctc tcctttttac aaactttaga aagtcaaaca ttttacttct caattatagt
    12541 tccaaaagcc aacttcaaca atctttagtt ggtggctttt agagttttct tgaactgtca
    12601 attgttgagt ttttggttcc taaacgccaa cctcaaatag acaaaagctc aacttttatg
    12661 aaaaaagtgt ttcacgtgaa acatattaaa cgttaaactc aacctttttt cgctctaaac
    12721 accctcgccc cttattataa gccatttttt agacctttaa cccaatctta aacggactct
    12781 tgcctttaga aagacgtctt ttatcccctc aacacacagg tttatccaca gtttttgttg
    12841 aaaaacaaca caagatttaa gaaaaaattg acgtcttttt tgattaattg tgtttttgcc
    12901 tgaaaaatat tagaatgtga tgacgttgtc tcccgaataa cagaaagacg aatttagcaa
    12961 cggttctttt gtaatcaaaa tatgtaaaat gcccctgtag tttaatggac cccgtggttg
    13021 agacaacggg gcgagaagtg gagttgaggt aagtttagag atagttcatt tgtaatcaaa
    13081 atatgtaaaa tgcccctgta gtttaatgga tagaactgga gacttctaat ctcccaatcg
    13141 aggttcgatt cctcgcgggg gcacaaaaga aacaacaacg gcctagtctg cgataccacg
    13201 atcagcctag agagcatatt tcggacgata aaaaagtcta ggacggacct agtccaaacc
    13261 tgtctgcgtt tttatcagca taaatcagcg ttagctcttc ggtctagtct gcgttctctg
    13321 ccggtttttc gtcaaaatac ttattgaagt tttcttcctt taactttatt tgacggtagg
    13381 ctctggataa tcggtctcgg tcaaccaaag attctgtcat ttgcgcgctt tcctcttcct
    13441 caactttaga ttcaaaccag tagaaaagaa ggaggtgcaa aacagtcacc aaaataaagc
    13501 caacaaaaaa caaactgatt atcattttcc aaaacgcaaa aggattaaag actttttcaa
    13561 acgcgccttc ggaaaagtta atttttattt tgggcaacca aagttttttc atcttatttt
    13621 atactttcca atttaacgga aacatccatc aaccaatttt cacctccgtc cgtggctgat
    13681 tttttataaa tggaaaactt attaaaaaat aatttgtgag gcaagttttc taataacaac
    13741 tggaactgca gcaaattgcg gaaattgcct tcgccagtga agctgacgac aatatccggg
    13801 ctatcttgcg cgccggtaac cttcagggca acgtctgttt gtctggccac cgattcaatt
    13861 tcctcaataa aatcaaccgt catgtcttta tcaataaact gcccctcaac ggccgcaagc
    13921 tgtgtttcca ggctgcccca aaagcgcctc aaagattcca agtcagactc agagaaaaga
    13981 gtctcggccg aggcttccac cttttctggt gttttttgcg cttgccaaat cagccaaaac
    14041 aaaaaactcc aaagcaaaag acaaaccagg cagacggcaa gcataatgta aaaaatgttt
    14101 ttttcttttt tcattttaat ccaaaaggtc tttcactaaa ttcgtttcca actccagcga
    14161 aaactgacta tctttatcat taatcaagtt gttaattggc gactcaacag atttaaacat
    14221 tttgttttgt ttcagcttgg ccacataatc caaaagcgac tgcctgtctc cggcccggcc
    14281 aagcgcggcc acattacata tttttgtttg agcttggcac tccagggcca aatggtggag
    14341 gcttatcccc tcccctttgt cattaaccgc ggccaaaaca acttcagagg gcaagattgc
    14401 atcttcgacc ggcaaaataa tttctagttt tttatttaat gtttggattt cttttaccaa
    14461 tgattgatcg gctggcgaca actcggccaa acttttctct ttttcttttt ctccgatccc
    14521 gccaaaagat aagtggagat aaaccgccgc caaaaaaaca caggccacaa aaatcagacc
    14581 agaaatcatc gtggttacca ccaccaacct gcggtaaata aatcggcgac gattttcttt
    14641 tttgtttttt tcaggcaata ggttgaacat aataatttta acgcttgaga tggctatttt
    14701 ttagggctaa tccaagcgct gtcacgtaac gcatagcgtt gttataatca ataaccggta
    14761 attcatcttg cttcaaatta aaattgaccc atggattgcc caggacaacc ggcacctcca
    14821 agtgagtttc cagatattcc ctaagaccgg gcaaggttga ctgaccacca accaaaatca
    14881 ctctgtcaat agacgaccgc ttgccttcat ccttgtcata atgattctgc caataactaa
    14941 agcgttgctt tatttcatcc tttaaggccg agacgggtgg catcagagca gaaaaaactt
    15001 cttggttttc ttgagaacgg agcaaaccct tttcttcttt taacttatta gccgcgaccg
    15061 cgtccaaacc cagggccttt tgcaaacaaa aagtaatatt ggaacctcct agaccaataa
    15121 tggaagtaaa gaccacctgg tcggcctgca ccaccgaaaa accagtgtgg attttgccaa
    15181 tatccaccat catagatgtt cccgggtcgc cctctttgac caatgcgcgc gcaatcgcct
    15241 gcgcctctat ctcaaacaga agcggttcta gaccggccgc agtaaaaatc tccaaataat
    15301 catccacaat cgctttgggc agaacggaaa ctgaaacctc ggcgccgttt ttttccactt
    15361 cgcgcgccga acttatcatc tcaaaatcaa acacgcattg gtcgggcggc aaaggcacat
    15421 attcttcaag ctgcaattct atactgccac gcaattcact ttttttcata ggcggcattt
    15481 tcattcggac aataaaagct tcgtcttccg gcaaagaagc gaagatgtaa ttaagcccca
    15541 tttctttttt taattccgac aaaaccttaa tcgcttctgt cttatttttt atgcggcctt
    15601 cctcaatcac gcctgccggc atcggtttct cgccataacg ttttacttta atctgcccat
    15661 tatgactaag ctcaacatat ttaaccgaac ggtcggaaat atccaaaccg gcggccggca
    15721 ccaagagata gtcgggcacg gggaaaaatt caaaaaaaat tttgctcttc attccttgtt
    15781 attataaaac aaaacaaaac cccacgccag atgacgcggg gcaaaaccaa aaaattgtcc
    15841 gagaattgac aattcaacca aggtctcttc tctcaaaatc tttccgagct tcaagtttga
    15901 gctgagacaa aactctcgcg cgcctcgcca aaacaaacac caacaaaacg aggattgcga
    15961 tgggcaacaa cgacactgct cggaaaggtg ctcctttaac aaggaggcca atttccccaa
    16021 gactccacaa caaggccatg aacgcggccc ccatgagcga aaacagctca tcaacggtct
    16081 tggctatttt ttcctgaaga cccttattca taaccctctc ctccaggccc ttgggttaat
    16141 tttgagacca aattcccaac cccaggttag caaaggcaac taaaaagtaa aggcgactgg
    16201 ttaaacgacc tccgcgttcc cctgccggtc aatatagctt ttcaagatca aggcggccgc
    16261 gcgcgcgtcg gtcaaagggt cagtttgttc ttcatctata atccgcttag cttcccaaga
    16321 agaaaaaaat tctctttccc gcgctagagg caaaccaatt ttttgtttca acaaatcttc
    16381 aaacttctca atctcgcgca ttagcggatt cggccgacca gacaaatcaa gtgactcgcc
    16441 gaccacaatc aagccaacct tttcgcgcgc gcacaattct ttgattttgc caaccaaaaa
    16501 tttgtcgttc ggccaagtgt tatgcgggaa agcaaaacgg ccgccttcat cagaaagcgc
    16561 caaaccgact tttttgtgcc cataatcaat ccccaacaat cgcataatta aagtgactga
    16621 cgcaagtcag cgcaaaattg agagcccaaa tcggcgctga ggttttggtt atagacaaaa
    16681 gccaagctat ctctattgta ttgataagcg ttcacttcac agtcggccaa ggcgccatca
    16741 aaatcaacat tccagctgcc agcataaatt actttttgtt ccggcttcca attcacgctg
    16801 gcgccgtttc ggactgaagc tccttccgat aaaataatcg gcccctcctc accagcggca
    16861 tcaataaatt tggccaaaac cggcttgtcc ccctgccaaa taaacaacgc caaatcatcg
    16921 gtgtaagcac cccccgaacc aatcgccacc aaagcttccg gcactccatc gccagtcagg
    16981 tctgcttcgc ggacaacttg aaccggtcgc aactgtcctt cagcggaaat atcttcagtt
    17041 tctttcaaaa ttgtttttat tttagatatt tgcttagccc aaactaaatc ttttgatgat
    17101 ggttcaagac atgggcaaaa acgcagagcc agtcccacca ctaaaagcaa aattgccgcc
    17161 gtcaaactaa ttaaaatttt ttttctcata aataagacct taacttgcca ataaaataat
    17221 tataccacta aaaacaattt aagccgtaaa caaaataaaa ggggctccga gagccccgct
    17281 aaaaaactga ccttggcacc tagcctgcca gagcagaccc aagctgttgc aaaccgaaac
    17341 tccgcaactg ggagccatcg gcatcgtaaa tgacctgggc tttcaaacag ctccgtggca
    17401 cctcaaggaa ccaggagtgg tctgaggacc agacctcctc tggcaaggac acgagccggt
    17461 tccaacagtc ttgattgatt ttcctcttgg cgtcatcggc atattgccac tgtttcgcca
    17521 aaaagacgta acaccggtaa cgaagctcct cttcaaaaag ggtgtctatg acgactgcca
    17581 gatgcaggtc ttccaatctg gcaaccaacc cccatttttc aaagaggtgt ttgagcgcca
    17641 ccacacaagc tgtctcttcg gcgggaacta ggctggccga acagttcaag cagtgggggc
    17701 tctctttctc cttctccttg tccttttttc ccatttttcc cataaagacc aaacggtatt
    17761 tggtatccaa gggaaaagca aggactatct cctgcggttt attccccatt agccatacct
    17821 cctttctggt tttattaaag cataagagga cacaatttaa caagctagag aggccgggga
    17881 aacaagaagg caaaattcgt tgcctctcgt ttcaattttt ccctcaactt gtcgcccttg
    17941 gttgttttat caaactttca agccatcttt caaggttttt aaaaagaatt ttctattatt
    18001 tttatttact gacaaaaaat tcaaccacca cgtaagccag ccaaaaaagc aagagcatcg
    18061 ccccttcttt tttcgtaacc gtacgaccgg aacgcataaa cctcaacaaa aatatcgagg
    18121 ccacaaccat aaacgtgccc gtcacataaa caattctggc cggaaagtaa aacggactaa
    18181 taagggcaag gaccccgata acgattgtcg cgtctgccaa aacagacccc aaaatatcgc
    18241 cgattgccaa gctgtctttc ttttgtctta cggccttaaa cgaatacaaa aactccggca
    18301 tggtagtccc caagctaaca accaacatac cgacaagaat aggtgtgatg ttgagcatct
    18361 cggccaaagc agtcgcggac cttaccgtga aatgcgcgcc aataagcaac atggccatag
    18421 ccgaaagcaa aaaaaggata tttttgtatt tgccgttgcg attgctaaac acctttgacg
    18481 cttccgtgcc gtttctaaac acaaaataat agaaaatcag cccggtaatt accaaaatgg
    18541 cgccgtcaat acgggaaaaa tgtccattta gcccaagaat tatgggaaac attaagaaaa
    18601 gcgggtacaa gtccacattt tttaatattt tgctctcaat ttttatgctt cggccggaca
    18661 gcaaaatcaa gagcgcaaaa ataagagaca ggtcagcaat atttgaacca aacaatgttc
    18721 caagcccaaa ttccggaatc ccctgaagcg cggaatttat tgagataatt gtttcgggca
    18781 aaattgaaat aaaagcaaca accacaaaac caataacata acgagagaga ctaaaatgtt
    18841 ccgcgagttt ggcggaatat ttagtggcaa gcgttgcgcc tttcactacc aaaaacagtg
    18901 agaaggcaaa aattaaaagg gtttccaaca taaatcactt catcaaacta tcaaatgagg
    18961 cccccgctgg cgggtttttc ttttaatgtt ttgtcttgtt ttataatttt ataattagca
    19021 agaatataaa cttcttgctg tcactgttca ttatattaaa tatctacgat ttcgcaaaaa
    19081 aataaagcgc caatttgtct ttgagaaatt tgacctgatt tggcgcgcgg agagcaaagc
    19141 agcagaatcc ccctccccct gcccgccaca ccttgcctga ccaaccaaaa cggcgcttga
    19201 gcgaaagccg aaacaaaaaa caatttaagt tttgcccatc tttggcgggg caactttaaa
    19261 ttttggcaaa aagaacgaga tagttatacc cgaatttttt ggcgcatccc gggcaaaatg
    19321 aaaattcgct gatgattggg gttggacaat ttttgttcca acccttgcct ctggccatag
    19381 tcccagccaa tgattttttt cgtcttgata tcaaggtcaa tataaaatct ggtattgata
    19441 tcaattttct tcgccataat ttgttgtcgt tgggttattt gtttttttct taataccgat
    19501 aaatacaatc attccggcaa atactgccag acacagaatc gcggcgccga taactgtcgg
    19561 atattccccg gcaaatccgc tttcagcaag atgcttataa agaatcgctg cgtaagccca
    19621 aataagcacc agcccatagg caatcgcgcg gtcgtaaagc attcgccatg agccaatcag
    19681 tgctccgacc aaaaggatga caatggtcca gaaaacttcg gaaaggccaa acctattcca
    19741 gccaatgctg accaaaaaaa cggtaatatt ggcgattgtc gccaccgtaa tccagccaaa
    19801 ataaacgcta aacggcaagc gcgccagcga tttttctttt tggccaagat tgccaacccg
    19861 caaaatatta gccagccgaa taagcgtata caacaatccg gccatcagca gaaccgagag
    19921 ccagatgacg tcataatgcc aggcaaatat ccaagcggca ttcaataaag cattaacgat
    19981 aaacaggcgg ttaattcgcg ccactaattc atttttcccc tgtcccaatt gataaatgac
    20041 ataaatcgcc aaaagtgtgt aaatcagtcc ccaaatagaa aaggcgtagc cagccggcgc
    20101 gaacagattg ggatagctgt ccgatatttc cccggtttct cgtccggcaa tcggaagcca
    20161 caccgccaga gcattgacga caaccatcac gatatatgcc cccagggtgg aaatttttaa
    20221 caattttgtg ttcatagttt ttatttattt aataattaaa aataaaattg gactattcca
    20281 aatacctctc ttcatacaat tgaggcacct gacactgcac aaaactcacg caatcattgg
    20341 gagttttgat atcaacagca ttgatacact tgatcatttc tttgggccaa gtcgggcatt
    20401 cttccatgca agtattgtaa gcatcggtca aatcagccgc cgtcacatct tcagtatagg
    20461 cggcgcagtc tttgtgcctt tggcaagcgt cagaacactt atcagaaatg acttttttat
    20521 tttcttcttc cgggcacagg gttttcagtt cctgtaaaag attttttatt ttttgttcaa
    20581 gctctgccat ttttatgatt ggctcattcg agtccgcaat attaatttcc ggcagaggcg
    20641 gcacgttgga atctaaagtg ggcacttccg aacctggccg gccggtattt aattccggca
    20701 attccgggac atattcaact ggtcttccca aatcatctac gacttggata tttatatctg
    20761 gcagagacgg cgcatcagag gtcagctccg gcacttttga gccgggcacc acgatgacat
    20821 ccggacccag ctccggcaaa ggtggaacat cggaggtcag ttctggtact tctgaacccg
    20881 gccgaccttc gggtgtaacc gaggttggtc ccccttcggg caattggggc acattatcca
    20941 gccagtcttt tttcctctcc gccaaatctg ccagcccatc ttcaagagat tttatctgtt
    21001 tggccagacc ttttttatcg ccacaatccg cgtttgcggt tttggcttgc tccgcctcta
    21061 ttaacaagct ttcataagct gaaactattt tttccggatt gtccaaggga ttttggggtt
    21121 gtgaatcgcc aaacttattt aataaaagat aagccgaaac gcccaaaata gctacggcga
    21181 cgacaccggt taaaattttt tggatagtac tcatatgatt attatataaa tataattaaa
    21241 attatttaac ctaccttccc aaaatttttc tcaactcctc aatgaccgct ttttcccagg
    21301 ctttcattgc aaaccaacca atgagcggct tggcgataac tcgcagccat tttttatgga
    21361 tatccacctc gtaaacataa ttgatttttg tcccgccttc aactttttcc aataaaagct
    21421 cttcgtgccc tgacgtgcca aatttggggc tttcattatc agaaatgaac cccctctccg
    21481 gaatgatgct agttttcatt ttgaccggga atgtttggcc gaatgttttt acttgagcgt
    21541 caatttccaa atgattgcct tcccgtttcg tgatgacgag tgacttggca actttgggaa
    21601 aatttttcgg cagattttca aaatcggtaa tgatgttgaa aacctcccct agcggagcct
    21661 tgactagcca tgaattttcc aggcggatat gcttcatata actaatttat aaacccccta
    21721 ccctgaatta acaagtttta tcttccccaa atttttctca actcctcaac catcgctttt
    21781 tctcaagcac ccagcgcaaa gaaccggcct atttgctccg gtaatccctc accacaaaac
    21841 cgccgcggat tctgattatt ttcactcttt gcttttcgcg ccatttgagc tcggtgatga
    21901 tttctttggg aatagttacc gccagactgg ttttacccag cttttgcagt ttgcgggtat
    21961 ttttgtctgc tagttttctc atatgtattt gagtatatac tttagtatat actttagtat
    22021 atacctatta tacaccattg gcggag
//
LOCUS       DAOOLO010000006        19814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_2738_length_19814_cov_5.990637, whole genome shotgun sequence.
ACCESSION   DAOOLO010000006 DAOOLO010000000
VERSION     DAOOLO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19814
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2738_length_19814_cov_5.990637"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLH96_00745"
     CDS             complement(<1..203)
                     /locus_tag="PLH96_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60333.1"
                     /translation="MSKTYKFLPEDFLELRKKIIELEQEVIEFGREVGESTEQSSETW
                     HDNATYDVARFNFEMTAAKLQEL"
     gene            complement(251..877)
                     /locus_tag="PLH96_00750"
     CDS             complement(251..877)
                     /locus_tag="PLH96_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60334.1"
                     /translation="MIKLLIEYSKEFELERIESTINRLDWYLSNKYSLSSLSFPKNID
                     VDNLKEIKKEELVEAVGEEYDESKFVTTAESIVQMYEKYEVKLEDFIKSIGLSVIPEV
                     KVSLTNYGIGGSYHLPNEIIVNINRFFSIGLIRNILHEIIHLHIQALIDQYKISQWNK
                     ETIVNLLFEKAFPEIYKEPNIPMDTTKIRKIFEENFPNIKKVISLISE"
     gene            complement(920..1996)
                     /gene="dnaJ"
                     /locus_tag="PLH96_00755"
     CDS             complement(920..1996)
                     /gene="dnaJ"
                     /locus_tag="PLH96_00755"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPB60335.1"
                     /translation="MAKDYYKTLGVEKNASKEDIRKAFHKLAHKYHPDKSTGDEQKFK
                     EVNEAYQILSDDKKRAQYDQFGSAGFGGQGGPGGGFDFSGFDFSGFNRDQNGSFEFDL
                     GDIFGDIFGGGRRTKVKRGRDISVDILITFKESIFGTERKLLINKVGLCDVCNGAGAE
                     KDTDVKKCATCDGSGQIKEAMRSILGTFQTAKTCPACHGRGKIPEKKCQACGGDGVRK
                     KDEEVRVKVPAGINNGEMIRMSERGEAVADGQNGDLYIKIHVERDELWRRSGNDLLAD
                     IKIKLTDAILGTDYKLKTLEGDLTLTIPTGIAYGEMLRVKGKGVPSERGRRGDLLVRV
                     TFPTPGKLSRKAKKIIEDLREEGL"
     gene            complement(2046..2402)
                     /locus_tag="PLH96_00760"
     CDS             complement(2046..2402)
                     /locus_tag="PLH96_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB60336.1"
                     /translation="MPNSLRERTYNFTLKVINLSSKLPNNRASWIISDQLIRSASSIG
                     ANHVEAQAASSRLEYKKFFEISLKSANETKYWLSLLKDAKIIDREKVEPLIKEVDEIC
                     KMLGQSVITLKSKVSS"
     gene            complement(2571..4505)
                     /gene="dnaK"
                     /locus_tag="PLH96_00765"
     CDS             complement(2571..4505)
                     /gene="dnaK"
                     /locus_tag="PLH96_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPB60337.1"
                     /translation="MAKIIGIDLGTTYSAVAAVEGGQPKIIENAEGARTTPSVVAISK
                     AGERLVGLIAKRQAITNPKNTIYGIKRLMGHKFEEPHVQRDKDISPFAINKADDGGVL
                     VKMGERDYRPEEISAMILQKLKNDAEAKLGEKIEEAVITVPAYFDDSQRKATKDAGEI
                     AGFKVRRIINEPTAAALAYGLDKKKDEKVVVYDFGGGTFDVSVLDIIGGEEEQSIQVK
                     STDGDSHMGGEDIDQKIIKWIADEFKKETGLDISHDELALQRLKEAAEKAKHELSTSM
                     QTDINIPFITSDSSGPVHLNKTMTRATLEDLAKEFIDRSIEITKRAVGASGYQISDIN
                     EIILVGGQIRMPAIQEAVKKLFGKEPNRTINPDEVVAIGAAIQGGIFQGDVKDVLLLD
                     VTPLSLGIETMGGVATKLIERNTTIPTSRSQIFSTAADNQTSVEIHVVQGEREMAADN
                     KSLGRFILDGIPPAPRGLPQVEVAFDIDANGILSVKAKEKSTGKEQSIRIEAKTSLSK
                     EDIERMKKDAEAHAEEDKKKREMVEVKNVAEQMIYTAEKALKDTDGQPAGEKRIPEEI
                     RRDVEDKITVLKGVKDGQDLEAIKNATSALSTEMQKIGESMMKTQSQEPPKEGGSNNE
                     DKKDDGQGGNEGNVRDAETK"
     gene            complement(4565..5164)
                     /locus_tag="PLH96_00770"
     CDS             complement(4565..5164)
                     /locus_tag="PLH96_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB60338.1"
                     /translation="MSKQTDKNKENELSEADEIEFEENLEDRAPEAASVNSKLKKLRE
                     ELKTCQTERGDYLAGWQRAKADYLNLKKEVEKQRGETAKWVKEDLFGEFLSLADSFEM
                     AMSNKEVWEQAPENWRKGVEYIYQQLKNIFQNHGLKEICPQVGDKFNPTEEHSIAIVP
                     AEFPDQDDVIDEVVKKGYKINEQILRPAQVKVRHWSEKP"
     gene            complement(5349..5753)
                     /gene="rpsI"
                     /locus_tag="PLH96_00775"
     CDS             complement(5349..5753)
                     /gene="rpsI"
                     /locus_tag="PLH96_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB60339.1"
                     /translation="MSTVTKDKYIEAIGRRKTSIARVRLYSGKGEIVINDRPFAEYFP
                     TEELKNRAKNQLADIKNLDKFLVSVKISGGGISSQAEAFRLGLARAIVKADEKTKSEV
                     KKAGLLTRDGRKKERYKFGLKKARKSPQWSKR"
     gene            complement(5758..6111)
                     /gene="rplM"
                     /locus_tag="PLH96_00780"
     CDS             complement(5758..6111)
                     /gene="rplM"
                     /locus_tag="PLH96_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPB60340.1"
                     /translation="MKNKEVTIDATGKAIGRVASEAAVALRNKNSAEFERHLTPAVKV
                     SIINASLMDVSNKKKKTKVYHHYSGYPGGLKEETLEHVIDKKGYREALLRAIKGMLPD
                     NKLRAVAMKNLTITE"
     gene            complement(6113..6463)
                     /gene="rplQ"
                     /locus_tag="PLH96_00785"
     CDS             complement(6113..6463)
                     /gene="rplQ"
                     /locus_tag="PLH96_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPB60341.1"
                     /translation="MRHQKTGKKFSRKIGVRKALLRSLMSNLILKEKITTTETKAKET
                     RRLIEKLVTKAKVDTVANRKLVTENLGSPLRVNKLFKEIGPRYAERAGGYTRITKLPK
                     RTSDGSSMAVIEFV"
     gene            complement(6481..7482)
                     /locus_tag="PLH96_00790"
     CDS             complement(6481..7482)
                     /locus_tag="PLH96_00790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPB60342.1"
                     /translation="MPDFNIIIPSKPRVIAEDEFSGVYEIEGLYPGYGHTLGNSLRRI
                     ILSSLPGFSITSLKVDGVDHEFSTIDGVKEDVINIILNLKRIKFKVTGDDSQVVKLRV
                     KGPKTVTAGDLDLPGQVEVINKEQEIATVTDKNTELAIEMTLDKGLGYVSKDILKKDK
                     VEVGTIAVDATFTPIRKASYEVENMRVGNRTDFNKLRLSIETDGTLTPREAMEKSIRI
                     MIDQLKAIIGFVEEEEEIESDAVIKEGGSLYDDLEYHSAIRRPGDNSIDENILKTRIE
                     DLDLSVRTIKALTGASIRTVSGLARKKESDLLEISGLGDKGIQEIKRALGSLGITLK"
     gene            complement(7523..8137)
                     /gene="rpsD"
                     /locus_tag="PLH96_00795"
     CDS             complement(7523..8137)
                     /gene="rpsD"
                     /locus_tag="PLH96_00795"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPB60343.1"
                     /translation="MKIGPKYKICRRVGDRVFGKCQTTKFTISGPDKKRRTGKRPKGA
                     PSEYSLQLLEKQKARFTYGVTERQFAKYVAGIKKHEDSNRAISMFKTLETRLDNVIFR
                     LGLAPSRALARQLVSHGHILVNNRRTRVPSTQVRVGDKISIRPQSKNNGVFQNLTEKT
                     KDYTIPEWLSLDLEKGEGVVKGEPIAGKAETTINFELILEFYSR"
     gene            complement(8179..8607)
                     /gene="rpsK"
                     /locus_tag="PLH96_00800"
     CDS             complement(8179..8607)
                     /gene="rpsK"
                     /locus_tag="PLH96_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPB60344.1"
                     /translation="MGKKRVIKKGGQDNTSRSAANQKTSKKKLDRGILYVEATYNNTK
                     LLLTDPSGKAYAWSSSGSLGFSGSKKGTPFAASKVGELLADRALAMGLKDADVIIKGV
                     GAGRESAMRAFSAKGINILSIKDRTPVPFNGPKPPKPRRV"
     gene            complement(8621..8998)
                     /gene="rpsM"
                     /locus_tag="PLH96_00805"
     CDS             complement(8621..8998)
                     /gene="rpsM"
                     /locus_tag="PLH96_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPB60345.1"
                     /translation="MMRIAGITLPNKRIEYALTAIYGIGLPRAQEILAEAKIDFGKKS
                     DDLTDDEENAIRKVIEDYKIEGDLKRERSANIKRLRDIKNYRGLRHQRGLPARGQRTK
                     TNARTLRGKKKTMGSGKRKVEKK"
     gene            complement(9016..9132)
                     /gene="rpmJ"
                     /locus_tag="PLH96_00810"
     CDS             complement(9016..9132)
                     /gene="rpmJ"
                     /locus_tag="PLH96_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPB60346.1"
                     /translation="MKIRSTIKKRCAQCKMVKRRGHLYIICKNKPRHKQKQA"
     gene            complement(9144..9392)
                     /gene="infA"
                     /locus_tag="PLH96_00815"
     CDS             complement(9144..9392)
                     /gene="infA"
                     /locus_tag="PLH96_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPB60347.1"
                     /translation="MEIDKKKTPDNSPKKEDIVYGVVIEALPNTLFKVKLDNEPDKEI
                     LAYLAGKMRINRIRVMVGDRVQMVMDPYGNRSRIVRRG"
     gene            complement(9470..11998)
                     /locus_tag="PLH96_00820"
     CDS             complement(9470..11998)
                     /locus_tag="PLH96_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60348.1"
                     /translation="MSFLSRKKGEGDLVALIDIGSNQISGAIFRTGQKNKTSISSSIL
                     FFTQTELSPQRSIAFKDYLKEASQALEKVLKALKASHHKPQAVFCFLSSPYFITRTKD
                     IVWRSEQKAPITTKKIGELSVGSAKDFADKHPSLYPEILNDRTIIIENEIMSVVLDGY
                     KGDNLTRGVAGKVEISQYVSLGSETLLGYFKNMVASFFPQAPVKFQTFTFAAYSVFKD
                     LMTGEKDFFLIDAAGDITDILVVNDGVLAEHRSFPFGRRQILKKLAEFSGTVPAEAES
                     LLKLLTESKTSQSANARVTGALEKIFIHWSTLFKNTISQIAETVFIPSRVYLIGYDSS
                     DLLLADWIKRIELSKFLLSSENYLSVGLIDKKLLTYEPINSLGQVTNTFILAEALFCD
                     KLYKNPNSFYFINSLKNMQDIVKNKKSLREIFPEVGASKMKSGITNTPPPTSSLSSRT
                     NPSPRRRFRFAGWLVLLVVLLIVGVGGFVLSNVFARAIVNIQPKQGQLDVSGTHVATR
                     EGDEGLKFVLAEDVEEVDTATVAASGSEKVEIKASGRIVIINKDSSTSQTLVEKTRFQ
                     APNGNIYRIAESVTVPGTHKNEKGETVPGQLEVTVYADKAGSAYNLGLADFTIPGFAK
                     TPKFEKITAKSKTPISGGFIGERPKISEADMANLEESMSGSLQAKVIEKIKQQVPEDY
                     VLFDDAVLTRFSTEIATSSESGSDKVVVRGKVTATGILFNRADLSQYLAKAIPGWTGE
                     DKVDIANMENLGFTLLDKENLNPESLTSISFNLQGIAHVVWQFDENALKDDLQKADKD
                     DYNDVFESYPEIQKAVIVFRPGWVRSVSQNDAKIEIKIEEKAFE"
     gene            complement(12039..12947)
                     /locus_tag="PLH96_00825"
     CDS             complement(12039..12947)
                     /locus_tag="PLH96_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60349.1"
                     /translation="MEKPQPNPESKKKSVFGHFREPIKPQEEGGLDEILAKAQGFQRQ
                     EDELTGKIDDKIREISSLFLGPQEDLPRPTVEAIEEVIEKSAKFKYKGPEETSRLDAE
                     LKRSLQKTEASLTTKESDSFARLEALVKDLISFNERDDFHPETAPIVGQLEDLTTIDF
                     QAMGEEVKSSDSNEYIINRESVSADLSKLSPLSLGLNGLAGFKLSEIFNRVIKNDPSR
                     YYFPSCDYLLACRSGSADVFREGTLILPGFFIRGQDGRWKVLGLKYNEQYRCLLFYFF
                     DLNQILDEEESREIRLMVFEKEKRDD"
     gene            complement(12961..14871)
                     /locus_tag="PLH96_00830"
     CDS             complement(12961..14871)
                     /locus_tag="PLH96_00830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPB60350.1"
                     /translation="MISEEIRQKFLTFFEKRGHKIIPSASLVPADYGDEGTLFTTAGM
                     QPLIPYLLGRPHPAGARLVDVQKCVRTGDIEDVGDNRHLTFFEMMGNWSIGDSTAENG
                     IGAGYFKQESINWSLEFLTSKEEGLGLDPKRFYVTIFRGEDGLPRDEEAIKIWQAAFA
                     QAGLEANIAGEDEVVGENIRIIPLGKEDNFWIAGATGPCGGDTEIFYDTRPTDGPLVG
                     KFGEMVDSFRLIEVWNNVFMEYEKMPDGSYKPLKQKNVDTGMGLERTTAVLNNVGVFQ
                     TDIFAPILAEITAHSNQDDEQAKRIIADHLRTAVFMLASGVGPANTDRGYILRRIIRR
                     LVRYSDKIGATDNLSEKLVDLVVAKYENFYPELATEKDKIIVELKKEEEKFRQTLAAG
                     IKIIDKEFAGKLNEVIDTAENMTSPLLTGEFFFNLYQTYGIPLELGLEEVKNRGLKLV
                     KGIEEKLKTEFGDRLQKHQELSRAGADQKFKGGLASESKQVVRYHTATHLLQAALKEV
                     LGPEVAQRGSNITDERLRFDFAYPAKLTDDQKNAVENLVNEKIKADLPMNRVELPKTE
                     AEKTGATHLFGEKYGETVTVYYTGDDVASAWSKEFCGGPHVSRTGELGHFKIKKEEAI
                     AAGVRRIKAVLE"
     gene            complement(14970..16088)
                     /gene="mnmA"
                     /locus_tag="PLH96_00835"
     CDS             complement(14970..16088)
                     /gene="mnmA"
                     /locus_tag="PLH96_00835"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB60351.1"
                     /translation="MALNNNKKVPKTVYVGMSGGVDSSVSAALLKKAGYKVVGVFIKP
                     WDPADDPETDWGIACNWRVERREAMRAAAHLDIPLITLDASGEYKEKVVDYLLSEYRA
                     GRTPNPDVMCNKQIKFGIFYNWAIAQGANFVATGHYCQLKAQSLKRKTKDFKLVVSKD
                     KNKDQTYFLWNLKKEQLPHILFPVGGYEKEEVRKLAKKFGLPNAKKKDSQGLCFIGQI
                     DFKKFLSGQIPLQTGAVLNEAGEIIGHHDGAIFLTIGERRGFVIEKKTPDQKPYFVIA
                     KDVKKNTVTVSSEPNKTKKMNAIFEINEINWINEVPRVGQKYQARFRYRQPLQTGQFT
                     KVTQEEAVFCFDESQLKPSGQSLVIYDGDVCLGGGAIR"
     gene            complement(16340..16633)
                     /locus_tag="PLH96_00840"
     CDS             complement(16340..16633)
                     /locus_tag="PLH96_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60352.1"
                     /translation="MKNPFVNALLAVLYIAIIALFLFNAPKIESTPSVLAPIVMLSLL
                     TFSVAVMGFLFFFQPLQLYFEDKKKEAIKSFLTTLLTFGLLTLIFAGILFYIQ"
     gene            complement(16771..17325)
                     /locus_tag="PLH96_00845"
     CDS             complement(16771..17325)
                     /locus_tag="PLH96_00845"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB60353.1"
                     /translation="MEKKQKLAFIDLETTGLDPEKHEILEIGGVVVWHNIDDRGDNSF
                     QEIGEFEYKVKPKHLETASPEALRINHYNEADWLFAGELNQTLKDLSKKVEGAVMVAH
                     NVAFDWGFLSAACFRENIDLKMDYHKLDLLSVAFAKLYDKDWTPKLNLWALAEHFGLQ
                     NKKAHSALADARVAYEIYQKLMTG"
     gene            complement(17424..>17690)
                     /locus_tag="PLH96_00850"
     CDS             complement(17424..>17690)
                     /locus_tag="PLH96_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPB60354.1"
                     /translation="VDATFEYYLGKTTFDLTKTDLKSESPYNTYVNKGLPPTPIGNPG
                     WDAIVAAIFPEESDYWFYLSDHDGNIHYAKTFEEHKANRVRYGI"
     assembly_gap    17691..17790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17791..18583)
                     /gene="mltG"
                     /locus_tag="PLH96_00855"
     CDS             complement(<17791..18583)
                     /gene="mltG"
                     /locus_tag="PLH96_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPB60355.1"
                     /translation="MIEDNTTTQNLKLSEDKKQIVATVGQYVLTALILVFILFNLTFV
                     FPPYTFPDEVYFRVESGETAGEVAENLYQQSLVRSPKTFKALIFLLGGQKKIQAGSYY
                     FSEPISALAIAMRLTNRFLGHEPYRITFFEGASAAKIAKAISQEAPDFNEELFVELGT
                     HWEGYLFPDTYFFSKLDGPADMIKVMRDNFDTRIKEVEGDIKATGKTMNEIITMASII
                     DREVRTEKDRHLVSGILWKRIASGMPLQVDATFEYYLGKTTFDLTK"
     gene            complement(18586..>19814)
                     /locus_tag="PLH96_00860"
     CDS             complement(18586..>19814)
                     /locus_tag="PLH96_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60356.1"
                     /translation="FFSQTWYWISLALPFTVYFLPFVLAYALWESYLAFIRERYINKE
                     ERILLEVKIPKEISKSPAAMELLMHVFYQTYEGELVDQYIKGTVRSWFSLELASFGGE
                     IHFYFNIPKFFKDLVEAQIYSQYSEVEIFEVDDYTQQVNFGQPDSPWEIFGWEWKFAK
                     ADIYPIKTYYDFGLEKDPKEEYKVDPMTPLMEFLGSIRQDEQVWFQFLVMGGKDRFPI
                     KNKKAKWYNFFSKVEMKGWKDASQEELQKILKREEKGEDIASFVKLTMSPGERLLAEA
                     IERHLAKLCFDVGIRAIYASKEGIRPIVKVGVGNSIKQFGSPGMNGFKVAATTSYNQP
                     WEDYKGIRARRLKKLMFKYFRERAYFYPPASKKPMVMTTEELATLYHFPGQAVTTPSL
                     SRIPSKRGEPPVNLPL"
BASE COUNT         5022 a         4540 c         4046 g         6106 t
ORIGIN      
        1 atcaattctt gtagtttggc ggcagtcatc tcaaagttaa aacgggccac gtcgtaagtg
       61 gcgttgtcgt gccaggtttc agagctttgc tcggtagatt cccccacctc gcgcccaaat
      121 tcaatgacct cttgttccag ttcgataatt tttttccgta actctaaaaa atcctcaggc
      181 aaaaatttgt aagttttgct cataaaatta ttttaaactc atataaaact ttacaccgag
      241 accaatatgt tcattcgcta attaaagaaa taaccttctt aatattagga aaattttctt
      301 caaatatctt tctgatttta gtagtatcca ttgggatgtt aggttccttg taaatttcag
      361 gaaacgcctt ttcaaagagg aggttaacaa ttgtttcttt attccactga cttattttat
      421 attgatcaat taaggcttgg atgtgaagat gaattatctc atgcaggata tttcttatca
      481 atccgatact gaaaaaacga tttatattta ctattatctc gttaggtaaa tgataactac
      541 ccccaatacc ataattagtt aaagagacct taacttctgg tattaccgac agaccaatac
      601 ttttaataaa gtcttctagc ttgacctcat atttttcata catttgtaca atcgactcgg
      661 cagtagtgac aaacttactt tcgtcgtatt cctccccaac ggcttcaacc aattcctcct
      721 tttttatttc tttaagatta tcaacgtcaa tgtttttggg gaaggataaa gagcttaaac
      781 tatatttgtt gctcaaatac caatcaagtc tgttgatagt actctcgatt ctttctaatt
      841 caaattcttt tgagtattct attaaaagct taatcatttg ggttatttca ttatttatct
      901 taaataacac accccaattc tatagccctt cttctcggag gtcctcaatg atttttttgg
      961 ctttgcggga gagtttgcct ggggtcggga aagtaactcg aaccaagaga tcgccacggc
     1021 ggccgcgttc gcttggtacg cctttgcctt taacccgcaa catttcgccg taagcaattc
     1081 cggttggaat agttaaggtt aaatccccct ccagagtttt taatttgtag tcagtgccca
     1141 aaatcgcatc tgtcaattta attttgatat cagccagcaa atcattgccg gaacggcgcc
     1201 aaagttcatc tcgttcaacg tgaattttga tgtaaaggtc gccattttgg ccgtcggcga
     1261 ccgcttcccc tcgctcggac attctaatca tctcgccatt atttataccg gccggcactt
     1321 taacgcgaac ttcctcgtct tttttgcgga caccatcgcc gccgcaagcc tggcattttt
     1381 tctcgggtat tttgccgcga ccgtgacagg cgggacaggt tttcgcggtc tggaaagttc
     1441 ccaagatgga acgcatggct tctttgattt ggccgctccc gtcacaggtg gcgcacttct
     1501 tgacgtcagt gtctttttcg gcgccggcgc cattacaaac atcgcaaagc ccaactttat
     1561 tgattaaaag ttttcgttct gtgccaaaaa tcgattcttt gaaagttatc aaaatatcca
     1621 ctgaaatatc tcgtccgcgt ttgactttgg ttcggcgacc acccccaaag atgtccccaa
     1681 agatgtcccc taggtcaaat tcaaacgagc cattttggtc acggttgaaa ccagagaaat
     1741 caaaaccgct gaagtcaaaa ccgccgcctg gaccaccttg gccgccaaaa ccggcgctgc
     1801 caaattggtc atactgcgcg cgttttttgt catcagacaa aatctgatag gcttcattaa
     1861 cttccttaaa cttttgttcg tcgccggtag atttatccgg gtgatattta tgcgctagct
     1921 tgtggaaggc tttgcggata tcttctttag aggcgttttt ctctacgccc aatgttttgt
     1981 agtaatcttt agccataata tagatgagct caaaacttaa aacgcaaaac gcaaaattta
     2041 atcttttaac ttgaaacctt ggattttaaa gtgataacgc tctggccaag cattttacaa
     2101 atctcgtcaa cctctttaat taaaggctca actttttctc ggtctattat cttagcgtcc
     2161 tttaataaac ttagccaata ttttgtttcg ttagcgcttt ttaaggaaat ttcaaaaaat
     2221 tttttatatt ccagtctgga gctggccgct tgagcttcaa catggttggc tccaatggaa
     2281 ctagcagatc ttattagttg atcactaata atccaacttg ccctattgtt tggaagtttt
     2341 gaactcaagt tgataacttt caaggtaaag ttataagttc tttctcttag actgttgggc
     2401 atagtttgaa ttttgtgttt tgcgctttaa gcttaaataa tagtaccatt aaaaggattt
     2461 ttgagcaaga atgcgtaatg cttcaatagt ctaaaacccc tggcgcgctt acgcgccagg
     2521 ggttttagtt aaggttattg attttttagc tctacgcttt gagttttgcg ctacttcgtc
     2581 tccgcgtctc tcacattccc ctcgttgcca ccttgcccgt catctttctt gtcttcatta
     2641 tttgacccgc cttctttcgg tggctcttgt gattgcgtct tcatcataga ttcgccgatt
     2701 ttttgcattt cggttgaaag agcgcttgtg gcatttttta tggcctcaag atcttgacca
     2761 tctttgactc ctttgaggac ggtaatctta tcttcaacat ctcgtctaat ttcttcaggg
     2821 attctttttt ctccggctgg ttggccatcg gtatccttta gagctttttc ggcagtataa
     2881 atcatttgtt cggcgacatt tttgacctca accatttcgc gctttttctt gtcttcctcc
     2941 gcgtgcgctt cagcgtcttt tttcattcgc tcaatatctt ctttggataa gctggtcttg
     3001 gcttcaatcc ggatggattg ttctttgcca gtggattttt ctttggcttt gactgacaaa
     3061 atgccgttgg cgtcgatgtc aaaagcgact tccacctgcg gcaaaccgcg cggcgccggc
     3121 gggatgccgt ctagaataaa acgaccaaga gatttattat cagctgccat ttcgcgttcg
     3181 ccttggacga catgaatctc aaccgaggtt tgattatctg ccgcggtgga aaaaatttgc
     3241 gaacgagaag tcgggatcgt ggtattgcgt tcaattaatt tcgtggcgac gccgcccatt
     3301 gtttcaatgc cgagcgaaag cggggttaca tccagcaaga gcacgtcttt gacatcgcct
     3361 tggaaaatac cgccttggat cgcggcgcca attgccacca cttcgtccgg attgatggtg
     3421 cggttcggtt ctttgccgaa taattttttg acggcttcct ggatggccgg cattctaatc
     3481 tgaccgccaa ccaaaatgat ttcattgata tcggaaatct gatagccgga ggcgcctacc
     3541 gcgcgcttag tgatttcaat agagcggtca ataaattctt tggccaagtc ctcaagagtg
     3601 gcgcgtgtca tagttttgtt gaggtgaacc gggccggaag aatctgaggt aataaacgga
     3661 atattgatgt cggtttgcat ggaggtggag agctcatgct tggctttttc cgcggcttcc
     3721 tttaggcgtt ggagagccag ctcgtcgtga ctgatgtcca agccggtctc ttttttaaac
     3781 tcatcggcaa tccacttgat aattttttgg tcaatatctt cgccgcccat atgcgagtcg
     3841 ccatcggtcg atttaacctg aatactttgc tcttcttcgc cgccgatgat atccaagact
     3901 gaaacatcaa aagttccgcc gccaaagtcg taaacgacaa ctttttcgtc tttcttttta
     3961 tccaaaccgt aagccagagc ggcggctgtc ggttcgttga tgatgcggcg gactttaaag
     4021 ccggcaatct cgcccgcgtc cttggtcgct ttgcgttgag agtcatcaaa gtaggccgga
     4081 acggtaatca ccgcctcttc tattttttca ccaagtttgg cctccgcgtc attcttcagc
     4141 ttttgcaaaa tcatggccga aatttcttcc ggacggtaat ctctttcacc catcttgacc
     4201 aacactccac catcgtcggc tttgttaatg gcaaatggtg agatatcttt gtcgcgttgg
     4261 acatgcggtt cttcaaattt gtgacccatc aaacgtttga tgccgtagat ggtatttttg
     4321 gggttagtga tagcttgtct tttggcgatt aaaccaacca gacgttcacc cgccttggag
     4381 atagcgacaa cagacggcgt tgtgcgcgcg ccctcagcat tttcaataat ttttggttgt
     4441 ccgccttcca cggcggcaac agcggaataa gtggtgccca ggtcaatacc tatgattttt
     4501 gccatatatc ttgaattgtc taatttttaa taaatttatt tttaaatgga ccgtttgttt
     4561 ttatttaagg tttttccgac cagtgtctga cttttacctg agccggacgt aaaatttgtt
     4621 catttatttt ataacccttt tttacgactt cgtcaatcac atcatcttgg tccgggaact
     4681 cggcgggaac aatcgcgatg gagtgctctt cagtgggatt gaatttatcg ccgacttggg
     4741 gacaaatttc tttcagaccg tgattttgga aaatattttt cagttgttgg taaatgtatt
     4801 ctacaccttt gcgccaattt tccggcgctt gttcccagac ttctttgttg ctcatcgcca
     4861 tttcaaaact gtcggccaaa gacaaaaatt ctccaaacaa atcttctttg acccacttag
     4921 cggtttcgcc gcgttgtttc tctacctctt tttttaggtt gaggtagtca gctttggcgc
     4981 gttgccagcc agccaggtag tcacctcgtt cagtttggca agtcttaagc tcttctcgca
     5041 gtttctttaa tttactgttg accgaggccg cttccggcgc gcggtcttcc aagttctctt
     5101 caaattctat ctcgtcggct tccgatagct cgttttcttt atttttgtct gtttgttttg
     5161 acatacatta ataagaataa gtaaaacact ttatatagag ttaaacggat aattcatcct
     5221 cattataact agttaaaagc ttttgtgcaa ataccaacta tgtttaaaaa taaggatttt
     5281 tcccgcccaa aggttcctaa cgggaagtcg gggcccaaaa aaaccggcca aggccggttt
     5341 ttgagttttt aacgtttgga ccattgtggc gacttgcgtg cttttttgag gccgaactta
     5401 taacgttctt ttttgcggcc atctctagtg agcaaaccgg cttttttgac ctcggacttg
     5461 gttttttcgt cagctttgac aatcgctcta gccaagccca aacgaaaggc ttcagcttga
     5521 gaagaaatcc cgccgcctga tattttgacg gaaaccaaaa acttatccaa attcttgata
     5581 tcggccagtt gattcttggc gcgatttttt aattcctcag ttggaaaata ttcagcaaat
     5641 ggtctgtcat tgatgacaat ttcgcctttg ccggaataga gacgcactct ggcaattgac
     5701 gttttgcgtc ggccgatcgc ttcaatatat ttgtcttttg ttacagtact cataatctta
     5761 ttcggttatc gttaaatttt tcattgccac cgcgcgcaac ttgttgtcgg gcaacatgcc
     5821 ttttatcgct cgcaaaagcg cttcgcggta acctttttta tcaatgacgt gttccaaagt
     5881 ttcttctttt agaccacccg ggtaaccgct gtagtgatgg taaacttttg tttttttctt
     5941 cttattagaa acatccatta gactggcgtt gatgatggaa acttttaccg ccggcgtgag
     6001 gtggcgttca aactcggcgc tatttttgtt gcgcaaagca acggccgctt cagaggcgac
     6061 acgcccaatg gccttaccgg tagcatcaat tgtaacttct ttgtttttca tattagacaa
     6121 attctattac cgccattgaa ctgccgtcac tagttcgttt tggcaacttg gtaattcggg
     6181 tatagccgcc cgcccgttca gcataacgcg gtccgatttc tttaaacaat ttgttaaccc
     6241 gcaacggtga accaaggttc tcggtaacca acttgcggtt agcaacggta tcaaccttgg
     6301 ctttagtgac cagcttttct atcaaacggc gggtttcttt ggccttggtt tcggtcgtgg
     6361 taatcttttc ctttaagatt aagttggaca tcaaagaacg aagcaaagct tttcgcacgc
     6421 ctattttccg actgaatttt ttacctgttt tttgatgtct cataattttg ttgtttctta
     6481 ttacttaagg gtgataccca gactgcctag ggctcgctta atctcttgga tacccttgtc
     6541 tcccagccca cttatttcta gcagatctga ttcttttttt ctagccaggc cgcttacggt
     6601 gcggatactc gcgccagtca gcgccttgat ggtgcgaaca gacaagtcta agtcttcaat
     6661 tctggttttc agaatgtttt cgtcaatact attgtcgccc ggtcggcgaa tcgcgctgtg
     6721 atattccaaa tcatcataca aactgccccc ctctttgata acagcgtctg attcaatttc
     6781 ctcttcttct tccacaaaac cgatgatggc cttgagctgg tcaatcataa tccgaatgct
     6841 tttttccatc gcttcgcgtg gcgtaagagt gccgtcggtt tcaatactta ggcgaagctt
     6901 gttgaagtcc gtgcgattgc cgacgcgcat attttctacc tcatagctgg ctttgcgaat
     6961 tggcgtaaaa gtggcgtcaa ccgcaattgt gccgacttcc accttgtctt ttttcaaaat
     7021 atcctttgaa acgtagccca aacctttgtc tagggtcatc tcaattgcca gctcagtatt
     7081 tttgtcggta acagtggcaa tttcctgttc tttgttgata acttctactt ggcccggcaa
     7141 atccaaatcg ccggcggtta cagttttagg acctttgacg cgcagtttga ccacttggct
     7201 atcgtcgccg gttaccttaa acttgattct cttcaagttt aagatgatgt tgatgacgtc
     7261 ttcctttacc ccgtcaatgg tggaaaactc atgatcaacc ccgtcaactt ttaaagaagt
     7321 aatcgaaaaa ccaggcaaag acgaaaggat aatccgccgt aaagagttac ccagggtgtg
     7381 gccgtaaccc gggtaaaggc cttcgatttc ataaacgccg ctgaattcat cttcggcaat
     7441 gactcgcggc tttgaaggaa tgatgatatt gaaatctggc atagttattt aagctattaa
     7501 aaattaaaaa tcactattaa atttatcggc tgtaaaattc caaaattaac tcaaaattga
     7561 tggtcgtttc cgccttgccg gcaattggct cacctttaac aaccccctcg cctttttcta
     7621 ggtcaagcga caaccattcc ggtatggtgt agtcttttgt tttttcggtc aggttttgga
     7681 agacaccatt atttttactt tgtggacgaa tggagatttt gtcgccaact cgaacttgcg
     7741 tagacggaac gcgggtacgg cggttattaa ccagaatgtg accatgcgaa acaagttgtc
     7801 tagccaaggc gcgtgatggc gctaagccca aacggaaaat cacattgtcc aacctggtct
     7861 ctaaggtttt aaacatgcta atggcgcggt tgctgtcctc gtgttttttg attccggcca
     7921 cgtatttggc aaattgtctt tcggtgacac cataggtaaa acgagccttt tgtttttcca
     7981 aaagttgaag agaatattca gacggcgccc ccttaggtcg cttgccggtg cgtcgttttt
     8041 tgtctggtcc agaaatggta aatttcgtgg tttggcattt gccaaaaacc cggtcgccga
     8101 ctcgcctgca aattttgtat tttggtccga ttttcatggt aataaaagtt tgagattaga
     8161 aattggaagt tggataaatt acacgcggcg cggctttggc ggttttggtc cgttgaacgg
     8221 aaccggcgtg cggtccttaa ttgacaaaat attgatacct ttagcagaaa aagcgcgcat
     8281 tgctgactcg cgaccggcgc caacaccttt aataatgaca tcggcatctt ttaggcccat
     8341 cgccaaagcg cggtcggcca aaagttcgcc aaccttgctc gccgcgaaag gcgtgccttt
     8401 tttggaacca gaaaaaccaa gggagccact ggatgaccaa gcgtaagctt tgccagaagg
     8461 gtcggtcaat aacaacttgg tgttattgta agtggcttcc acgtataaaa tgccccgatc
     8521 caattttttc ttggatgttt tttgattagc ggcactgcga ctggtgttgt cttggcctcc
     8581 ttttttgata actcgtttct ttcccataac tcaataatta ttatttcttt tcaaccttac
     8641 gtttgccgga acccatcgtc tttttcttgc cacgcaaagt tcgggcgtta gtcttggttc
     8701 gttgaccgcg agccggcaaa cctctttggt gtctcagacc gcggtagttt ttgatatcac
     8761 gcagacgctt aatatttgct gagcgctctc ttttcaggtc gccttcaatc ttataatctt
     8821 ctatcacttt acgaatcgcg ttttcttcgt catcagtcag gtcatcactt tttttgccaa
     8881 aatcaatttt agcttcggcc aaaatttcct gcgctcgagg taaaccgatg ccataaatgg
     8941 ctgtcagggc atactctatt cttttgttcg gcaaagtgat accggcgatt ctcataatat
     9001 taaaataaaa attatctagg cctgcttttg tttgtggcga ggcttatttt tgcaaataat
     9061 ataaagatgg ccccttcttt tgaccatttt gcattgagcg catctttttt tgatggttga
     9121 cctgattttc atctttttgc tatttagccc ctcctcacaa ttcggctacg atttccgtag
     9181 gggtccatga ccatttggac cctatcccca accataactc ggatacggtt aatccgcatt
     9241 tttccggcta ggtaagccaa aatctcctta tctggctcat tgtccaactt cactttgaac
     9301 aaagtgtttg gcagagcttc tataacaacc ccataaacga tatcctcctt tttaggtgag
     9361 ttgtcagggg tctttttttt gtctatttcc attaccaaat tgacttggac atattaacaa
     9421 atttacgcct taaaagtcaa ggcaaatcga ccaatttaac cggcaggtgc tattcaaaag
     9481 ctttttcttc tatttttatc tcaattttgg cgtcattttg ggacacagag cgaacccacc
     9541 ccgggcggaa aacaattacc gccttttgaa tttcaggata ggattcaaaa acgtcgttat
     9601 agtcgtcttt atcagctttt tgcaggtcgt ctttgagggc attctcgtca aattgccaaa
     9661 caacatgagc gattccttgc agattaaagg aaatagaagt cagactttca gggttaaggt
     9721 tttctttgtc caacaaagta aaaccaagat tttccatgtt cgcaatatca accttgtcct
     9781 cgccggtcca gccggggatt gctttggcca agtattggct taggtcagcg cggttaaaaa
     9841 gaataccggt ggcggttact ttgcccctga cgaccacttt gtctgaaccg ctttccgatg
     9901 aggtggcaat ttcggtagaa aatcgggtca aaaccgcgtc atcaaaaaga acatagtcct
     9961 cgggcacttg ttgtttaatt ttctcgataa ctttagcttg caggctgccg ctcattgatt
    10021 cctctaagtt ggccatatcg gcttctgaaa tttttggcct ctcgccgata aacccgccac
    10081 tgatgggagt ctttgatttg gcggtaattt tttcaaattt aggggttttg gcaaaaccgg
    10141 ggatagtgaa gtctgctagt cccaagttat aagccgagcc ggctttgtcg gcataaaccg
    10201 ttacttccaa ctgtcccggc acggtctcgc ccttttcatt cttgtgagtg cccggcacgg
    10261 ttactgattc ggcaatccgg taaatattgc cattgggcgc ttggaaacgg gttttttcca
    10321 ccaaggtctg gctagtggaa ctatccttgt tgataatgac aattcggccg ctagctttga
    10381 tttctacttt ttcactaccg gaggcagcca ccgtcgccgt atccacttct tctacatctt
    10441 cagccaaaac aaatttcaag ccctcatcac cttctctagt cgctacgtga gtgccggaaa
    10501 catccaattg tccttgttta ggttgaatat ttacaattgc tctggcaaaa acattggaca
    10561 aaacaaaccc tccaacacca acaatcaaca aaacaaccaa taagactagc caaccggcaa
    10621 aacgaaagcg gcggcgaggg ctggggttgg tgcgagagga aagggaagag gtcggtggtg
    10681 gtgtgttggt tattcctgac ttcatctttg acgcgccaac ttctgggaaa atttcacgaa
    10741 gacttttttt gtttttgaca atatcctgca tgttttttaa tgaattaata aaataaaagc
    10801 tatttggatt tttatacagc ttatcacaaa acagcgcttc agccaaaata aaggtgttgg
    10861 taacttgtcc gaggctgttt atcggttcat aagtaagcaa ctttttatca ataaggccaa
    10921 ccgagaggta gttttcggat gataacaaaa acttggacaa ctcaattctt tttatccaat
    10981 cagccaacaa taaatctgag ctatcgtaac caatcagata aacgcgcgat ggaataaaaa
    11041 ctgtttcggc aatttggctg atagtgtttt tgaacaaagt tgaccagtgg ataaaaattt
    11101 tttccagcgc gccggtgacg cgcgcgttgg cagattgact tgttttactt tctgtcagca
    11161 acttcagcaa tgactcggcc tcggccggaa ccgtgcccga aaattcagct aattttttca
    11221 aaatttgtcg gcggccaaaa ggaaaagacc tatgttcggc caaaacgccg tcgttgacca
    11281 ccaaaatatc tgtgatatcg ccggccgcat ctatcaggaa aaaatctttt tcaccggtca
    11341 ttaaatcttt gaagacacta taagcggcaa aggtaaaggt ctggaatttg acgggcgctt
    11401 gcggaaaaaa actagccacc atatttttga aataacccaa aagagtctct gagccaagac
    11461 taacatattg gctaatctcc accttgccgg ccacccccct ggttaggttg tcgcctttgt
    11521 aaccgtcaag aacaaccgac attatttcat tttcaataat aatagttcgg tcatttagaa
    11581 tttccggata aagcgaaggg tgtttgtcgg caaaatcttt ggctgaccca acgctaagtt
    11641 cccctatttt cttggttgta atgggcgctt tttgttccga ccgccaaact atgtctttgg
    11701 ttctggttat aaagtaaggt gatgataaaa agcaaaaaac agcctgcggc ttatggtgac
    11761 ttgcttttag cgcctttaaa accttttcca aggcttggct tgcttccttg agataatctt
    11821 taaaagcgat agacctttgg ggcgataatt ctgtttgcgt aaagaacaaa atgctggaag
    11881 agatgctcgt tttgtttttt tggccagtcc gaaaaatcgc gccgctgatt tgattgctgc
    11941 caatgtcaat caacgccact aaatcgcctt ctcctttttt gcgtgataag aaagacactt
    12001 cttatattat aagattctcc tagaaaaaaa gaaagggatt aatcgtcgcg cttttctttt
    12061 tcaaacacca tgagtcttat ttcccttgac tcttcttcat caagaatttg atttaagtca
    12121 aaaaaataaa aaagaagaca tcggtattgt tcattgtatt tcaaaccaag aactttccag
    12181 cgaccatctt gacccctaat gaaaaaaccg ggcaaaataa gcgtgccctc cctgaacaca
    12241 tcggccgagc cagacctgca agcaagcaag taatcacagc tgggaaagta atatcttgaa
    12301 ggatcatttt ttatcacccg attaaatatc tcggataatt tgaatccggc cagtccatta
    12361 agccctaagc tgagggggct aagtttactt aaatccgcag agacagattc tcggttgatg
    12421 atatattcgt tagaatcgct cgacttaact tcttcaccca ttgcttggaa gtcgattgtc
    12481 gttaaatcct ctagctgtcc aacaatgggg gcagtttccg ggtggaaatc gtctctttcg
    12541 ttaaaactta tgagatcttt aactagggcc tccaacctgg cgaaagaatc cgactctttt
    12601 gttgtcaaag acgcttcagt tttttgaaga gaacgtttaa gttcagcgtc aagtctgcta
    12661 gtctcctccg gccctttata cttaaacttg gcactttttt ctattacttc ttctatcgcc
    12721 tcaaccgttg gccgaggcaa gtcttcttga ggcccgagaa aaagtgagct tatttctctt
    12781 attttgtcat caatcttgcc ggtcagttcg tcctcttggc gttggaagcc ttgggctttg
    12841 gccaggattt catctaaccc cccttcttct tggggtttga ttggttctct aaaatgccca
    12901 aagaccgatt ttttcttgct ctcaggattg ggctggggtt tttccataat aatatttaga
    12961 ttattccaaa actgccttta tccgtcgaac gccggctgct atagcttctt cttttttaat
    13021 tttaaaatga cctaattcac cagtgcgcga aacatgcggt cccccgcaaa attctttgga
    13081 ccaagcggaa gcgacatcat cgccggtgta ataaactgtg acggtctcgc cgtatttttc
    13141 accaaaaagg tgagtcgcgc cagttttttc ggcctcagtc tttggcaatt ccacgcggtt
    13201 cattggcaaa tcagctttga ttttttcatt aaccaaattt tcaactgcgt ttttttggtc
    13261 atctgtcaat ttggccggat aagcaaagtc aaaacgtaac cgttcatctg taatgttgct
    13321 gcccctttgc gccacttccg gccccaaaac ttcttttaaa gcagcctgta ggaggtgggt
    13381 ggcggtatgg tatctgacta cttgtttgct ttcactggcc aagccgcctt tgaacttttg
    13441 gtcagcgccg gcgcgtgata attcttggtg tttttgcaaa cggtcgccaa attctgtttt
    13501 taatttttcc tctattcctt ttactaattt caaaccgcga tttttgacct cttccaaacc
    13561 aagttcaagc ggaatgccgt aagtttgata gaggttgaag aaaaattcgc cagtcaacaa
    13621 cgggctagtc atattttctg cggtgtcaat gacttcattt agttttccgg caaattcttt
    13681 gtcaataatt ttaatgcccg ccgctaaagt ttgacggaac ttttcctctt cttttttaag
    13741 ctcaacaatt attttatctt tttcggtggc tagttccgga taaaaatttt catacttagc
    13801 caccaccaaa tccaccaatt tctcgctaag gttatcggtc gcgccaatct tgtcgctgta
    13861 acgcaccaaa cgcctaataa tccggcgcaa gatgtagccg cggtcagtat tggctggccc
    13921 cacaccggag gccaacataa agacagccgt gcgaagatgg tcggcaataa tccgtttggc
    13981 ctgctcgtcg tcttgattgc tgtgagcggt gatttcagcc aaaatcggag caaaaatatc
    14041 agtctggaaa acaccaacat tattcaagac ggcggttgtc ctctccagcc ccatgccggt
    14101 atcaacgttt ttttgtttga gcggtttgta actgccatcc ggcatttttt catattccat
    14161 aaaaacgttg ttccagactt caatcagacg gaaagaatcc accatttcac cgaactttcc
    14221 aacaagcggg ccatctgtcg gacgagtgtc ataaaaaatc tccgtgtcgc cgccgcatgg
    14281 accggtagcg ccggcaatcc agaaattgtc ctctttgcca agcgggatga ttctgatgtt
    14341 ttccccaacc acttcgtctt cgccggcaat gttcgcttcc aaaccagctt gagcaaaagc
    14401 cgcctgccaa attttgatcg cttcttcatc gcgcggcagg ccatcttcgc cccgaaaaat
    14461 tgtcacataa aaacgcttgg ggtccaagcc tagaccctct tctttgcttg ttaaaaattc
    14521 aaggctccaa ttaatagatt cttgcttgaa gtaacctgcc ccaattccgt tttctgcggt
    14581 ggagtcaccg attgaccagt tacccatcat ttcaaaaaaa gtcaggtggc ggttgtcgcc
    14641 aacgtcttca atgtcgcccg ttcgaacgca tttttgaaca tcaaccaagc gagcgccggc
    14701 cgggtgaggt cgacccaaga gataaggaat caagggttgc ataccggccg tggtaaaaag
    14761 ggtgccttcg tcgccataat cagccggcac cagagaggcc gaggggatta ttttgtgtcc
    14821 ccttttttca aaaaaagtca aaaatttttg acgaatttct tcagaaatca tgatttaatc
    14881 ttaacaaaac aacggcccaa gcgcaaaaaa actatgtatt tatctttaaa ttatctaaac
    14941 tctcggcccc tactatttta atttttacat tatcttatcg cccctccacc caaacagaca
    15001 tcgccgtcat aaatcaccaa ggattgcccg cttggtttaa gttgcgattc gtcaaaacaa
    15061 aaaacagcct cctcttgtgt gactttggta aactgaccgg tttgcaacgg ctggcgatag
    15121 cggaaacgcg cctgatattt ttggccaact cttgggacct cgttgatcca atttatttca
    15181 ttgatttcaa aaatagcatt catctttttg gttttatttg gctcagatga aacagtgaca
    15241 gtgttttttt taacatcttt ggcgatgaca aagtatggtt tttggtcagg tgtttttttc
    15301 tcaataacaa aacctcgccg ttcgccgatg gtaaggaaaa tcgcgccatc atggtgacca
    15361 ataatttccc ccgcttcgtt taaaacggcg ccggtttgga gcggtatttg tccactcaaa
    15421 aattttttaa aatcaatttg accgataaaa cagagcccct ggctgtcttt tttcttggcg
    15481 tttggcaacc caaatttttt tgccagtttc cgcacttctt ctttttcgta accgccgacc
    15541 ggaaacaaaa tatgcggtaa ttgttctttt tttaaattcc acaaaaaata agtttggtct
    15601 ttatttttgt ctttgctgac cactaactta aaatctttag ttttgcgctt taaactttgg
    15661 gctttaagtt ggcaatagtg ccctgtcgcc acaaaattcg cgccttgagc gatggcccag
    15721 ttatagaaga ttccaaattt aatttgtttg ttgcacatca catcagggtt tggtgtccgg
    15781 ccggcgcgat attcgcttaa gagataatca actacttttt ctttgtattc acctgaagcg
    15841 tcaagggtaa taagcggaat atcaaggtgg gcggctgcgc gcatcgcctc gcggcgttca
    15901 acgcgccaat tgcaagcgat accccagtcg gtctctgggt catcagccgg gtcccaaggt
    15961 ttaataaaaa cgccgaccac cttataaccg gcttttttta gcaaggcggc ggaaaccgag
    16021 ctatctaccc cgcctgacat gccaacataa actgtttttg ggactttttt attgttattc
    16081 aaagccattt cccgctaaca ttaccacgaa tttttgtttt tgaacacaaa aagagcctgc
    16141 ggaacctaaa ggttccgcag gcaacctcaa ctgcctttgc agcagtgagc aactctcgga
    16201 aaagtgaatc gcttcatttt atcaccccct ttccaagaaa agaacactaa tacccactta
    16261 cattataaca ttgtcgtcaa gtttttgaat aaccaaatca aatagaattc ttgagctcat
    16321 ttggtcgtct tcattcgccc tattgtatat aaaacaatat tccggcgaag attaaggtta
    16381 aaaggccaaa ggttaataag gtagttaaga aggactttat cgcttctttt ttcttgtctt
    16441 caaaatacaa ctgcagaggt tgaaaaaaga acaaaaagcc catcacggcc accgaaaaag
    16501 tgagcaagga cagcatcaca attggcgcca agaccgaagg cgtgctttca atctttggcg
    16561 cgttaaacaa aaataaggca attatggcga tatacaaaac cgcgagcaaa gcattaacaa
    16621 aaggattttt catagtttta atttaaattt ttaattttgt tattatacca catttctgcc
    16681 aaaaaagagg tccaacctcc tttgggcagg tttgttacgt agcatgttac gtaataaacc
    16741 aaacagttaa agtccttcct agtttaaact ctaccccgtc atcaatttct gataaatctc
    16801 gtaagcgacg cgggcgtcgg cgagggcgga gtgagctttt ttattttgaa gaccaaaatg
    16861 ctcggccaag gcccaaagat taagtttggg cgtccaatcc ttgtcataaa gtttggcaaa
    16921 ggcgacagaa agcaggtcta gtttgtggta atccattttt aaatcaatat tttcgcgaaa
    16981 acaggccgca ctcaaaaaac cccagtcaaa cgcgacattg tgcgcgacca tcacggcccc
    17041 ttctaccttc ttgctcaaat cttttaacgt ttgatttagt tcgccggcaa aaagccaatc
    17101 cgcttcattg taatggttta tccgcaaggc ttcagggctg gccgtctcaa gatgttttgg
    17161 tttgactttg tattcaaact cgccaatttc ttgaaaagaa ttgtctccac ggtcatcaat
    17221 attatgccaa acaacgactc cgccaatttc taaaatctcg tgcttctccg ggtcaagacc
    17281 ggtggtctca aggtctataa aagctaattt ttgtttcttt tccataataa attaagcgcg
    17341 acacgaacat acaaatgata gtaaatgtaa actgtaaaaa ttaaaataaa aaattgtctt
    17401 tttgctaaaa ccgataacct tccttatatc ccgtagcgaa cgcggttagc cttatgttct
    17461 tcaaacgttt tggcatagtg gatattccca tcatggtcag acaaataaaa ccaataatcg
    17521 ctttcttcgg gaaagatggc ggcgacaatc gcgtcccagc ccggattgcc gatcggagtt
    17581 ggaggcaaac ccttgttgac gtaagtgtta taaggcgatt cggacttaag gtcggtcttg
    17641 gtcaggtcaa aagtagtttt gcccaaataa tattcaaagg tggcatccac nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttggtcag gtcaaaagta gttttgccca
    17821 aataatattc aaaggtggca tccacctgca agggcatgcc ggaggcaatt cttttccaaa
    17881 gtatgccgga aacaagatga cggtcttttt cggtgcgaac ttcacggtca ataatagagg
    17941 ccatggtaat tatttcattc atcgtcttgc cggtcgcttt gatatcacct tctacttcct
    18001 ttatgcgggt atcaaaatta tcccgcataa ccttaatcat atcggccgga ccgtccagtt
    18061 tggaaaagaa gtaggtgtcg ggaaaaagat agccctccca atgagtcccc agctccacaa
    18121 acaattcttc gttgaagtca ggcgcttctt gactaatggc cttggcaatt ttcgccgccg
    18181 acgcgccctc aaaaaaagta atccggtatg gctcgtgacc caaaaagcga tttgtgagac
    18241 gcatagcaat cgccaaggcg gaaatcggtt cgctgaaata ataactgccc gcctggattt
    18301 tcttttgacc gcccaaaagg aaaatcaggg ctttaaaagt tttcggcgaa cgaaccagac
    18361 tttgttggta taaattttcg gccacctcgc cggcggtctc gccgctttcc acgcggaaat
    18421 aaacctcgtc cggaaaggta taaggcggaa agacaaaggt cagattaaat aaaataaaaa
    18481 ccaggatgag ggcggtcaga acatattgcc ccacggttgc cacaatttgt tttttatcct
    18541 cgcttagctt gaggttttgg gtcgtagtgt tatcttcaat cataactata gtggcaggtt
    18601 aactggcggt tcgcctcgtt tggacgggat acgcgacagc gatggagtag taactgcttg
    18661 gcctgggaag tggtagagcg ttgccaattc ttcggtcgtc attaccattg gttttttgct
    18721 ggctggcgga tagaaatagg cgcgttcacg aaaatattta aacatcagtt tttttaagcg
    18781 tctagcccta atccccttgt aatcttccca cggttggtta taactcgtgg tcgccgccac
    18841 tttaaaacca ttcataccgg gcgaaccgaa ttgttttatg ctgttgccaa cgccaacttt
    18901 gacaatcggg cggatgcctt ctttggaagc gtaaatagcg cgaatgccaa cgtcaaaaca
    18961 aagtttggcc aagtgtcttt ctatcgcttc agccaagagg cgctcgccgg gcgacattgt
    19021 caacttcaca aaggaagcaa tgtcttcgcc tttttcttca cgttttagaa ttttttgtaa
    19081 ttcctcttgg gaagcgtctt tccaaccttt catctctacc tttgaaaaga aattatacca
    19141 ttttgctttt ttatttttga ttgggaaacg atctttgccg cccatgacca aaaactgaaa
    19201 ccaaacttgt tcgtcttgtc tgatagagcc caaaaattcc atcaggggtg tcatcgggtc
    19261 aactttgtat tcttctttgg ggtctttttc caaaccgaaa tcataataag ttttgattgg
    19321 gtaaatatca gctttggcaa atttccactc ccagccgaaa atttcccacg gcgaatccgg
    19381 ttggccaaag tttacttgtt gcgtgtaatc gtcaacttca aaaatttcca cttcggaata
    19441 ctgagaataa atttgcgctt ctaccaagtc tttgaaaaac ttcggaatat tgaaatagaa
    19501 atggatttcg ccgccaaaag aggctaactc caacgaaaac caagaacgaa cagtgccctt
    19561 gatgtattgg tctaccagct cgccctcata agtttggtaa aaaacatgca tgagaagttc
    19621 catcgcggcc ggcgatttgc taatctcttt cgggattttg acttccagta aaatacgttc
    19681 ttccttgttt atgtatcgtt cgcgaataaa agccaaataa gactcccaaa gagcataggc
    19741 caaaacgaac ggcaaaaaat agaccgtaaa aggcagggcc aaagaaatcc aataccaagt
    19801 ctggctaaaa aagt
//
LOCUS       DAOOLO010000007        18501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_3084_length_18501_cov_6.738588, whole genome shotgun sequence.
ACCESSION   DAOOLO010000007 DAOOLO010000000
VERSION     DAOOLO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3084_length_18501_cov_6.738588"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /gene="ppsA"
                     /locus_tag="PLH96_00865"
     CDS             <1..1376
                     /gene="ppsA"
                     /locus_tag="PLH96_00865"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HPB60357.1"
                     /translation="KGLELARGVAIGSKIASGKVRVIRQAKEMSTFKPGEILVTEITD
                     PDWEPIMKIASAIITEKGGRTSHAAIVSRELGVPCVVGTGNATKVLKNGQQITVDCSS
                     GQEGLIYKGDLPFEVIEHRLNEIPTPRTKVTLNVGSPEEAMKNYYLPVAGVGLGRLEF
                     IITSYIKIHPNALLDFDKIKRSRDRASQKIAKQIEALTKEYSGKNKQDFYVNELTEGI
                     GMIGAAFWPNEVIIRFSDFKTNEYRTLIGGELYEPEEENPMIGWRGASRYYDPKFKAA
                     FALEVQALVKVRKELGLTNVVAMVPFCRTLEEGQAVVQIMAENGLDREKNKELKIYVM
                     CEIPSNVILADKFLDIFDGMSIGSNDLTQLVLGLDRDSNILAHIGNENDPSVKTLIGA
                     VIERCRARGKYVGICGQAPSDYPDFTEFLVKSGINSMSLNPDVIIKMLSVVERAEKIT
                     EGVVKKN"
     gene            1384..2400
                     /locus_tag="PLH96_00870"
     CDS             1384..2400
                     /locus_tag="PLH96_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPB60358.1"
                     /translation="MSKVIFFELEDWQKDYLKKNYPTPEDLVLIDGPLLADRLPAEKE
                     TEIISVFVDSRLDEKVLSHFPNLKLITTRSTGFDHIDLDLCRQKGIQVANVPSYGEET
                     VAEFTFALMLTLSRKVCESYDQVRVTGSFGLNGLRGFDLNGKTLGVIGTGRIGRNVIE
                     IAKGFNMNIIAYDKFPDEEYAKKMGYRYLPFVEVLTQADIVTLHIPYTTENHHLLNTE
                     TIAQMKKGAYLINTARGSLVETDALFKALKNGHLAGAALDVLEEEGIVKDEMNFLSNN
                     WEGPYDLKTVLQNHMLVDMPNVIVTPHNAFNTWEALKRILNTTIKNMTEFGEGRPVNI
                     VSES"
     gene            2531..3682
                     /gene="tgt"
                     /locus_tag="PLH96_00875"
     CDS             2531..3682
                     /gene="tgt"
                     /locus_tag="PLH96_00875"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB60359.1"
                     /translation="MFNFKIEKKSSDSLARAGVIETPHGVIETPAFVTVGTKATVKAL
                     TPEMVASLGASVVLANTYHLYLQPGDELVKAAGGLHQFMNWPGPMMTDSGGFQVFSLG
                     AAFGEGGVSKFVSGHDPNKKSKEQAGNIAKLAKIDEDGVAFQSHIDGSAHRLTPERSM
                     QIQHNLGADIIFAFDECTSPLAPYDYQKEAMERTHRWAKRSLDEHKRLGGPQALFGIV
                     QGGRHQDLRQESSRVIGGMDFAGFGIGGSFNKEDMGTAVSWVSQNLPENKPRHLLGIG
                     EPEDIIAGVKSGADTFDCVAPTRMARNGALLTARGRLNILNASCRCDLAPIEEGCGCY
                     TCQNYSRAYLAHLFRAKEMLAATLASIHNLYYLINLTKRIRRDVLEGRL"
     gene            3783..5828
                     /gene="uvrB"
                     /locus_tag="PLH96_00880"
     CDS             3783..5828
                     /gene="uvrB"
                     /locus_tag="PLH96_00880"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPB60360.1"
                     /translation="MKSKLKPAGDQPKAILALNKGLTKELGHQTLLGVTGSGKTFTMA
                     NVIAAQDKPVLVIAHNKTLAAQLAQEYRDFFPDNAVHYFVSYYDYYQPEAYVVASDTY
                     IEKDASINEEIERLRHASTQALLTRRDVVIVASVSCIYGLGSPSEYAKSHLFFKRGDQ
                     LSRSDLIRRLVEIYYERTNADLLPGQFRAIGNSVEVMPPGERAIVNIKIEGDKVAEII
                     ILDAVSRAIVRESKETFFFPAKHFITQEDERNRAIISIKAELEKRLVELNKEGKILEA
                     ERLKRRTNYDLAMIREVGYCNGIENYSRHLSGRAAGEAPATLLDYFPRQKNGSSDFLT
                     IIDESHVTVPQIRGMYAGDESRKKTLVEHGFRLPSALDNRPLKFPEFQERIGQVIYTS
                     ATPSEYEISESRQAAQEAGLKPELGIVEQIIRPTGLLDPMIEIRPVKASSSKNAQAYG
                     GQVADFIVEAEKEIKAGNRVIATTLTKKMAEDLSQFLREKKVKSEYLHSEVKTLERIK
                     ILTDFRKGKFDCLVGVNLLREGLDLPEVTFIGILDADKEGFLRSEVSLIQTIGRAARN
                     VDGRVILYAEVITGSMQKAIDETNRRRALQIAYNKKHQITPQSIQKKINDITETLESE
                     RARTVGEMLTVDSKLFGGNLKKVIKEKVKQLNEAVKELDFETAAIIRDEIRALEQKA"
     gene            6098..8752
                     /gene="uvrA"
                     /locus_tag="PLH96_00885"
     CDS             6098..8752
                     /gene="uvrA"
                     /locus_tag="PLH96_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPB60361.1"
                     /translation="MEDKIKIKGARTHNLKNINLEIPRNKLVVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLKQLPKPEVEEISGLSPAISIDQKSRSHNPRSTVATI
                     TEIYDYLRVLYARIGHPYCPVCGTKIEKLSNEEIGNFIRDEISKHSTASAVEIYAPVI
                     RGRKGEYYQLLYDLLEKGYSEVKIDGARHKLRERIVMARTKKHDIDVLVDEIELVGFL
                     SQKSESKLKKEVDRAITEAANERLSEAVERAIKEANGLVKVIIGGEEFLLSAKFACPN
                     DGFSFPEIEPRLFSFNSPYGACPVCHGLGTKHLFGNEPCPECLGARLRREALHVFIGS
                     IKEKSIKQAEDGKVKTKIERTGLNIVQLTALSISEATEFFASLKLTAKEKAISEVLLR
                     EIEARLKFLNDVGLDYIALDRRADTLSGGEAQRIRLASQLGSRLVGTLYVLDEPTIGL
                     HARDNDKLIKTLLELRDLGNSILVVEHDEDTIFSSDYLVDIGPGAGVHGGEIITSGWT
                     EKLLTAKKNNFGSLTVDYLRGEKEVPFPAKRRQNDKGSIKIRGGNIFNIKNLNVDIPL
                     GRLVAVTGVSGSGKSSLVYEILYKNLQGRLERRYRTNEVFNCKEFSGSEYLGRVIMVD
                     QSPIGRTPRSNPATYTGAFTFIRNLFASTALARARGWSPSRFSFNVQGGRCEACQGNG
                     EIAVEMHFLPTIFVPCDVCGGKRYEKDTLEVEYKKKNIYQVLKMTIEETLLFFNDIPA
                     IYDRLKTLNEVGLGYLELGQPANTLSGGEAQRVKISSELYRPQTERTIYLLDEPTIGL
                     HYEDVKNLIEILQKLVEKGNTVLVIEHNMEVIKNADYVIDMGPEGGNGGGQVVAAGTP
                     EEVAYASGSHTAKYLKRALNLNKRDKKQ"
     gene            8793..10127
                     /locus_tag="PLH96_00890"
     CDS             8793..10127
                     /locus_tag="PLH96_00890"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPB60362.1"
                     /translation="MFLRIDITKYKLPDKPGVYYFLGSSKLGKTTKDARGVLYIGKAT
                     SLKDRVRSYFNGDLGETRGPKIEQMLLQAADLKWQETDSVLEALILEAELIKKHQPPY
                     NTREKDDKSYWYVVITKEDYPRVLMRRGRELEKDLSRKYEIEKTFGPFPFSSELKAAL
                     KIVRRIFPYRDTCLLYSEKNKGLKVPGLRGCFNSQIGLCPGVCTGAIDKKTYRQIIKN
                     LRLFFEGKKEQVVKNLEKEMKIAVKAQNFEEAARWRDQIFALKHIQDVSLLKNTKTEN
                     FLFEAGAELVNRRDGKEKTVFRLEAYDIAHLAGAGTVGAMAVWEGRELNKSEYRLFKL
                     RGRSANKSDDTGNLAEILRRRFTHREWRLPDLVVVDGGQAQFNAAQKTLNQLNLSLPL
                     VAVVKDEKHRPREILGEKTMAKNHRRKILAVNADVHRFVLAYHRKILRQMLK"
     gene            10133..11128
                     /locus_tag="PLH96_00895"
     CDS             10133..11128
                     /locus_tag="PLH96_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB60363.1"
                     /translation="MDSYPSSLFDLFILLSSPTFWLVALLIFVAAAVLGYWYRGRSGR
                     VQMSIKEANLKTRQIKELEKINRELENKNRELYAKELELVMANKRLQSLEAAKSKFIS
                     VTTHQLRTPLAAIKWTFDMAVKGQLGKVNEEFKKFMEKGFQSTERVISIVNDLLKIDT
                     IETGKLDFVFKPTNIVDLINNVIGEFCNQVKTRQLEIIFEKPANRVPFVEIDENKIRM
                     VLENLIDNAIKYNLPNGKIFIKISDARLNSAESSLQISITDSGIGIPDGEEDKMFQKF
                     FRASNAIKQEPDGSGLGLYITREIIEKHHGAIWFERGKDGGTSFTFTLPLHQKKV"
     gene            11184..11552
                     /locus_tag="PLH96_00900"
     CDS             11184..11552
                     /locus_tag="PLH96_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB60364.1"
                     /translation="MTKKILIVEDDEFLRSLNAKRLEGEGFEVLIAVDGNEALTKLTE
                     IKPDLIFLDLLLPNVDGFEVLTKIKQDETTKNIPVIVFSNLGQAEDIEKAKKLGADDF
                     MVKANFTLDDVISKIKETLK"
     gene            11638..12618
                     /locus_tag="PLH96_00905"
     CDS             11638..12618
                     /locus_tag="PLH96_00905"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPB60365.1"
                     /translation="MSSSLIRVGVMRGGLGSEYEVSLKTGATVLEYLPREKYQPRDIL
                     ITKDGQWHMDGFGIEPARLRNSLDVVFNALHGEFGEDGQVQTLLDNTMLPYTGSGRLA
                     SALGMNKVAAKEIISRAGLKVPRGVHLKFKPETNAEAVAYDVFLKISPPWIVKPVGRG
                     SSVGVFLAKTFDDLVVAVSECFKISEVILVEEYIRGREATCGVVDDFRGHKTYPLLPI
                     EIAPPAGKKFFDYEAKYSGQSTEICPGQFSVGEKRELGELAAAVHKLLGLKHYSRTDF
                     IVSPRGIYILEVNSLPGLTAESLVPKSLAALGVPLPHFLDHVVSLALGKK"
     gene            complement(12640..13098)
                     /locus_tag="PLH96_00910"
     CDS             complement(12640..13098)
                     /locus_tag="PLH96_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60366.1"
                     /translation="MENSSPNPSFSICDIRIQKRVAECFAKSFSPENSFGKTESALVP
                     PPARILGRQNPERKTPFPFLEKIHPRQIRNARSVFSFGVAEALRGGGGTVHSFSFRSK
                     KVRANAKITPPNKLPSKEVEPPWGAELLRSLLRNMLRNKLYVKLTSQGPP"
     gene            13235..13447
                     /locus_tag="PLH96_00915"
     CDS             13235..13447
                     /locus_tag="PLH96_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB60367.1"
                     /translation="MESKQNKEIGDKIKVFRAKQGLTQDALARKCDIPYTTLTKIESN
                     VITKPSIQTVTKIAEGLGISIDALIK"
     gene            13452..14633
                     /gene="tyrS"
                     /locus_tag="PLH96_00920"
     CDS             13452..14633
                     /gene="tyrS"
                     /locus_tag="PLH96_00920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB60368.1"
                     /translation="MTKIEKLNKTEELLSRGVEEIIDRSSLENKLRSGKQLRIKLGID
                     PTSPNIHLGRSIPLLKLRDFQELGHKIILIIGDFTGLIGDTSDKDSERPMLLEETIKE
                     NLKTYIKQAAKIIDINTTEIYHNSKWLGELGYMDIGRQADIFSLSEFISRENISRRLN
                     EGKRVSLRELLYPLMQGYDSVEVRADVEIGGTDQRFNLLAGRDMQRLYNQKPQDILTN
                     PIIEGLDGRKMSSSFGNTVNLFDTPNDMFGKIMSLKDDFIIKYFTLLTRVDIKSISEY
                     DSSLKAGINPRDLKMKLAFEIVRMYCSEKDARVAEDYFIKTFSKKETPVNLPKFKPSK
                     YDIISILIENKLTSSRSDARRTIEQGGVKIDGVVIKDIHHVVSSGSILQKGKIKFVEI
                     I"
     gene            14630..15454
                     /locus_tag="PLH96_00925"
     CDS             14630..15454
                     /locus_tag="PLH96_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60369.1"
                     /translation="MNKNVIKICKSFIKEILEKEDWFRQLVNDKACDLLLLTGTATEQ
                     TVSLPLSEIENMEKQIDLWEKESISERGSVAQNLKTKLSETKEKLDKLVSLYLDGDIE
                     REIYLQRKDLLLRQKAKFEESFKDFGQQRKNWVEPLRSFVLSLREAVILEKGENYFAW
                     KEFFQKIGSNPVLKDKTLSCNWGELWEFTASAVGGKGFEKIDSAILASAKSLRFLNSD
                     NVSFGGHYTKKFGLISSRILMTNESAPPPLVPLACPAKNILRFSNLARDQFFLLKG"
     gene            15677..16264
                     /locus_tag="PLH96_00930"
     CDS             15677..16264
                     /locus_tag="PLH96_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB60370.1"
                     /translation="MNTEDIQLVSLAKQNPKEYEKLYTKYSDKVFNYFWYRTGHDKPL
                     SEDLMQETFLRAFQHLKRFNNRGYSYLTYLITIAHNVLVDHYRKPKSIPFEAIEDIPF
                     EITEDLERKSDAESLWRAIQDLPQSNRDKLLMKYQQDMSVRDIATATGTTENAVKLSL
                     SRTRKKLKDHPYVKDIVGFADTKRKQTRARFLDKK"
     gene            16343..17578
                     /locus_tag="PLH96_00935"
     CDS             16343..17578
                     /locus_tag="PLH96_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB60371.1"
                     /translation="MNHKDFVRQTAITVGVALVTTMLTGAGFLTWAKHNPSSFVQLIT
                     PSTRPNENATSTAFLYPSESILVDTVKKANPAVVAITISKNVPVYERYLERMPSPFED
                     FFGDDFFGGFTIPQLRQKGTELREVGGGSGFLVSADGYIVTNRHVVSDESAEYTVFTN
                     DGKKHEAKVIARDPALDLAVIKISGSGFHHLSFGDSDKLEIGQTVIAIGNALAEFRNT
                     VSVGVVSGLSRSITASDRRGGFEALDQLIQTDAGINPGNSGGPLINLSGEVVGVNVAV
                     ADGAENIGFALSANSVKSIVESVKTTGKIVRPYLGVRYIEINEEIKEKNKLSVDYGVL
                     VQRGVEVSDLAVIPGSPADKAGIVENDIILEIDGIKLTSDQSLAAIIRTKKVGDTVRL
                     KINHKGQEKIVAVKLEEMK"
     gene            17916..18419
                     /locus_tag="PLH96_00940"
     CDS             17916..18419
                     /locus_tag="PLH96_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB60372.1"
                     /translation="MKTEIQKRDNKNQALSRRDDFASPFSLIDRFFDDSVWDPFNMMT
                     PSLFRSRGLGNVSTAFPKVDVEETDNEIKVTANVPGIDPKDINVEVGDDYLSLSGKID
                     KENKDEKNGKVYRYEREYGEFRREFSLPARVNKDSIVAKSKNGVLMITLPKSEEEMKK
                     RVKIDVE"
BASE COUNT         5393 a         3601 c         4509 g         4998 t
ORIGIN      
        1 aaaaagggtt ggaacttgcc cgtggtgtgg cgatcggttc taaaattgcc agtggcaagg
       61 tgcgggtgat tagacaagcc aaagaaatgt ccactttcaa acccggtgaa attttagtga
      121 ctgaaattac cgacccggac tgggagccca taatgaaaat cgcgtcagcg atcatcaccg
      181 aaaaaggcgg gcggacaagt cacgcggcaa ttgtttcgcg cgaactaggc gtaccgtgtg
      241 tagttggcac cggcaatgcc accaaggttc taaaaaatgg ccaacagatt acagttgact
      301 gctcatctgg tcaagaaggt ttaatctaca agggtgactt gccgtttgaa gtgattgaac
      361 accgtctaaa tgaaattccg acaccgcgga ccaaggtgac gttaaatgtt ggctcgcctg
      421 aagaggctat gaaaaattac tatttgccgg tggccggtgt tggcttggga cgtttggaat
      481 ttattatcac atcttacatt aaaatccacc caaacgcttt gcttgatttt gacaaaatca
      541 aacgttctcg cgaccgcgct tcacaaaaaa tcgcaaaaca aattgaagcg ctgaccaaag
      601 aatattccgg caagaacaaa caagattttt acgtcaacga gttgacggag ggaattggaa
      661 tgatcggcgc ggctttttgg cccaatgaag taattatccg cttttctgat tttaagacaa
      721 atgaatatcg cactttgatt ggcggtgaac tctatgaacc ggaggaagaa aatccaatga
      781 ttggctggcg cggcgcttct cgttattatg atccaaagtt taaggcggct ttcgcgcttg
      841 aagtccaagc cctggtcaag gtcagaaaag agctggggct caccaatgtt gtggcgatgg
      901 tgccgttctg ccgcaccctg gaagaagggc aagcggtcgt gcagattatg gccgaaaacg
      961 gtttagatcg agagaagaat aaagaattga aaatttatgt gatgtgtgaa attccttcca
     1021 acgttatttt ggcagataag tttttggata tttttgacgg tatgtccatc ggttcaaacg
     1081 atttaacaca gctggtgctg gggcttgacc gagattccaa cattttggcg catatcggca
     1141 acgaaaatga tccgtccgtc aaaactttga ttggcgcggt gattgaacgt tgtcgcgcgc
     1201 gtggtaagta tgtcggcatt tgtggtcaag caccttcgga ctatccggat ttcactgaat
     1261 tcttggtcaa aagcggtatt aacagcatgt ctcttaaccc ggacgtaatt attaagatgc
     1321 tttcggttgt agaacgggcg gagaaaatta ccgaaggcgt tgtaaagaag aattaatcca
     1381 agtatgtcaa aggttatatt ttttgagtta gaagattggc aaaaagatta cctcaagaag
     1441 aattatccga cgccggaaga tcttgttctc atcgacggcc ctcttttggc tgaccgtttg
     1501 ccggccgaga aggagacaga gattatttct gtttttgttg attcgcgctt ggatgaaaaa
     1561 gtgctcagcc attttcctaa cttaaaatta atcacaacgc gttctaccgg ttttgaccac
     1621 attgatttgg atttgtgccg ccagaaaggg attcaggtgg cgaatgttcc cagttatggc
     1681 gaagagaccg tcgccgaatt cactttcgcg ttgatgttga ccttgtcgcg aaaagtttgt
     1741 gagagctatg accaggtgag ggtaaccggc agttttggtc tgaacggctt gcgtggcttt
     1801 gatttgaatg gcaaaaccct aggcgtcatt ggaaccggcc ggattggccg caacgtgata
     1861 gaaatcgcca agggttttaa tatgaatatt atcgcttatg ataagtttcc cgacgaggaa
     1921 tatgccaaaa agatgggtta tcgttatttg ccgttcgtgg aggtgttgac gcaagctgat
     1981 attgtgactc tccacatacc ctacacaact gaaaatcacc acttgttaaa caccgagacg
     2041 attgctcaaa tgaaaaaagg agcttacctc atcaacaccg cgcgcggttc tttggtggaa
     2101 acggatgccc ttttcaaagc gcttaaaaat gggcacttgg ctggcgccgc gcttgatgtt
     2161 ttggaggaag agggaatagt caaagatgag atgaattttt tgagcaataa ttgggagggt
     2221 ccgtatgacc tcaaaacagt gttacaaaat catatgttgg ttgacatgcc aaacgtgatc
     2281 gttactccgc acaacgcttt taatacctgg gaggccctaa agcggatttt gaacacgaca
     2341 ataaaaaata tgacggagtt tggcgaaggg aggccggtta atatagttag cgaaagttaa
     2401 aagttaaaag tcaaaagttt caaggggaga ggcaaaacct ctccccttgg gcgtgcactc
     2461 tggcccttcg ctaggtgcga aatgcaaaat gctatatact atccattgtt atgtttaact
     2521 atccattgtt atgtttaatt ttaaaattga gaaaaagtct tccgatagtc tggcgcgcgc
     2581 gggagtgatt gaaacgcctc atggagtgat tgaaacgccg gcctttgtca cagtgggaac
     2641 caaggcgacg gttaaagctc ttacgccgga aatggttgcg tcgcttggcg cgtcggtggt
     2701 tttggccaat acttaccatc tttaccttca gccaggggac gagctagtca aggcggctgg
     2761 cggcttgcac cagtttatga attggcctgg cccaatgatg actgactccg gcggattcca
     2821 agttttttca cttggcgccg ccttcggcga aggcggcgtt tcaaaatttg tgagtggcca
     2881 tgatccaaac aaaaagtcca aagaacaagc aggaaatatt gccaagttgg cgaaaattga
     2941 cgaagatgga gttgcttttc aatcacacat tgatgggagc gctcatcgtt taactccaga
     3001 gaggtcaatg caaatccaac acaacttggg cgcggatatt atttttgcgt ttgacgaatg
     3061 cacttctccc ttggcgcctt atgattatca aaaagaagcg atggagcgca cccatcgttg
     3121 ggccaaacgg tcgttggatg aacacaagcg ccttggcggg ccacaggctt tgtttgggat
     3181 agttcagggg ggacgccatc aagatttacg ccaagaatca tcgcgagtga ttgggggaat
     3241 ggattttgcc ggctttggaa ttggcggttc gtttaataaa gaagatatgg gcacggcggt
     3301 tagttgggtg agccaaaatt tacctgaaaa taaaccaaga catcttttgg ggattggcga
     3361 accggaagac attatcgcgg gagtcaagag cggcgctgac acttttgatt gtgtcgcgcc
     3421 aactcgaatg gctcgtaacg gcgcattgct aactgcccga ggacggttaa atattttaaa
     3481 cgcttcttgt cgttgcgatc tggcgccgat agaagaagga tgtggttgct atacttgcca
     3541 aaattattct cgcgcctatc tcgcgcacct gtttcgcgct aaggaaatgt tggcggcgac
     3601 cttggcttca atccacaatc tgtattattt gattaatcta acgaaaagga ttagaagaga
     3661 tgttttagag ggtaggttgt aagacggaaa atggtggcgg ttgaccgttg tccagcttgt
     3721 tcgtgatttt atttttcatt atcttgtgtt aagcttgccg tgtgtctaaa aatgttttcc
     3781 aattgaaatc taaacttaag ccggccgggg accagcccaa ggcaattttg gcgctcaaca
     3841 aaggtctgac caaagagttg ggccaccaaa ctcttttggg ggtaactggt tcaggcaaaa
     3901 cttttacgat ggccaatgtg attgccgccc aagataaacc ggttttggtc attgctcaca
     3961 acaagactct ggcggctcag ttagcgcaag agtaccgcga tttttttccc gataacgcgg
     4021 ttcattattt tgtttcttac tacgattact atcaacccga agcttacgtt gtcgcgtctg
     4081 atacttatat tgaaaaagac gccagtatta acgaagaaat tgaacgttta cgtcatgctt
     4141 caacgcaagc tttgctcacc cgccgtgatg tggttattgt cgcctcggtc tcttgtatct
     4201 atggcttggg gagtccaagt gaatatgcca aaagtcacct cttttttaaa cgaggtgacc
     4261 aattgtcccg gagcgaccta atccgccgtt tagtggagat ttattatgaa cgaacaaatg
     4321 ccgacctctt gccgggacaa ttccgcgcga ttggcaattc agtggaagtt atgccaccgg
     4381 gtgagagagc gattgtgaat attaaaattg aaggagacaa ggtggcggaa ataataattt
     4441 tggacgccgt cagtcgcgcc attgttcgtg aatctaaaga aactttcttt tttccagcta
     4501 aacactttat tacgcaagaa gatgaacgga atcgggcaat tatttctatt aaagcagaat
     4561 tagaaaaacg cttggttgag ttaaacaaag agggtaaaat cttggaagca gaaagattaa
     4621 agcgccgaac caattatgac ctcgcaatga ttcgggaggt tggctattgc aacggaattg
     4681 aaaattattc acgtcatctt tctggccgcg ccgccggcga ggcgccggcc acgctcttag
     4741 attattttcc gcgccaaaaa aatggttcgt ctgacttttt gacgattatt gacgagtcgc
     4801 atgtgaccgt gccgcaaatt cgcgggatgt atgccggcga tgaaagtcgc aaaaaaacct
     4861 tggttgagca cggtttccga ttgccgagcg ctttggacaa tcgtccgctt aaattcccgg
     4921 agttccaaga aaggattggc caggtgattt atacaagcgc gacgccaagc gaatatgaaa
     4981 taagcgaaag tcgtcaagcg gcgcaagaag ccggtttaaa gccggagctt ggtattgtgg
     5041 aacaaattat ccgcccgacc ggtttgcttg acccgatgat agaaattcgg ccagtcaagg
     5101 cttcaagttc taaaaacgcc caagcttacg gcggtcaagt tgccgatttt attgttgagg
     5161 cagaaaaaga aattaaagct ggcaatcgag taattgctac aaccctaacc aaaaaaatgg
     5221 cagaggacct gtcacagttt ttgagagaga aaaaagtaaa atcggaatat ttgcacagcg
     5281 aggtaaaaac tttggagcgg attaagattt taaccgattt tcgcaagggc aagtttgatt
     5341 gtttggtcgg ggtcaatctt ttacgggaag gcctagactt gccggaggtg acctttattg
     5401 gcattttgga tgctgacaaa gaaggttttt tgcgctctga agtttctttg attcagacga
     5461 ttggccgagc ggcgcgaaat gtagacggtc gagtaattct ttacgcggag gtaattacag
     5521 gttctatgca aaaagcgata gacgaaacca atcggcgacg agccctgcaa attgcctata
     5581 acaaaaaaca tcagattact cctcaatcaa ttcaaaaaaa gattaatgac attacggaaa
     5641 ctttggaaag cgagcgggca aggacagtcg gtgaaatgct tacggtggac agcaagttgt
     5701 ttggcggtaa tttaaagaag gtgataaagg aaaaagtaaa acagttgaac gaagcggtga
     5761 aagagcttga ttttgagacc gcggcgatta tcagggacga aataagagct ctggaacaga
     5821 aggcttagca aaaaataaaa acggtgggtt cttgtttgtt atttgtaacc atggtacact
     5881 agaatagtgt cgtgtgttcc tcctttccgt ttggggtgtc tggaattagc cctgtaggcc
     5941 cagacacccc atattttttt agatgacgat ggtttcggta gaggtctctg ctcaacaagg
     6001 ccattcctcc cgtttttatt ttcctgtttt tatgttataa acacattatc accccgtggc
     6061 cgaatcatgg ggcgtttggg cgtaaaaagc taattttatg gaagacaaaa taaaaattaa
     6121 aggcgcgagg acgcataatc taaaaaacat caacctggaa atcccgcgaa acaaactcgt
     6181 ggtttttacc gggctatcgg gctccggcaa gtcatcgttg gcttttgata cgatttttgc
     6241 tgaaggtcag cggcgttatg tggaatcgct gtcagcttac gccaggcaat ttttaaaaca
     6301 gttgcctaaa ccggaagtgg aggaaatctc cggtttgtcg ccggcaatct ctattgacca
     6361 aaaatctcgt tcccataacc cgcgttccac ggtggcaacc attaccgaaa tctacgacta
     6421 tttgcgcgtt ctctacgcgc gaattggcca tccctattgt ccggtttgcg gcactaaaat
     6481 agaaaaatta tccaacgaag aaattggcaa ttttatcaga gatgaaatct ctaaacactc
     6541 caccgcgagc gcggtggaaa tttacgcgcc agttattcgc ggccgtaaag gagaatacta
     6601 ccaattgctc tatgatttac tggaaaaagg ttactcagaa gtaaaaatag acggcgcgcg
     6661 tcacaaattg cgcgaacgga ttgtgatggc caggaccaaa aagcatgata ttgacgtttt
     6721 ggttgacgaa attgaattgg ttggcttctt gagtcaaaaa tcagagtcaa agttaaaaaa
     6781 agaggtggac cgagcgataa cagaggcggc taacgagcga ttgtcagaag ctgtggagcg
     6841 ggcgatcaaa gaagccaatg gtttggtcaa ggtgataatt ggcggggaag aatttttgtt
     6901 gtcggctaaa tttgcctgcc ccaatgatgg tttttctttt ccagaaatag aaccgcgtct
     6961 tttttctttt aactcacctt acggcgcttg cccagtctgc cacggtttgg ggaccaaaca
     7021 cttattcggt aacgaacctt gtccagaatg tttgggcgcg cgtctgcggc gagaggccct
     7081 tcatgttttc attggttcaa tcaaagaaaa atcaatcaaa caagcggaag atggaaaagt
     7141 taaaacgaaa atagagagaa ccggactaaa cattgttcaa ctgactgcct tgtctattag
     7201 tgaagcgaca gaattttttg cttccttaaa attgaccgct aaagaaaaag ccatttctga
     7261 agttttgttg cgcgaaattg aagcgcgctt gaagtttctt aatgatgtcg gtttggatta
     7321 tatcgcgctt gaccggcggg cagatactct ttccggcggc gaggctcagc ggattcgtct
     7381 ggcctcgcag cttggctcac gcttggtcgg cactttgtat gttttagacg aaccgacgat
     7441 cggtttgcac gcgcgtgata acgacaaatt aatcaaaacc ttacttgagt tgcgcgactt
     7501 gggcaactcc attttggtgg tggagcatga tgaagacaca atcttttctt ctgattattt
     7561 ggtggatatt gggccagggg ctggcgtgca cggcggggaa attatcactt ccggttggac
     7621 ggaaaaactt ttaacggcca agaaaaataa ttttggttcg ctgacggtgg attatttgcg
     7681 tggcgaaaag gaggtgcctt ttccggcgaa gaggcggcaa aacgacaagg gttcaatcaa
     7741 aatccggggt ggcaatattt tcaacatcaa aaatctaaat gtggatattc cgcttggccg
     7801 cttggtggcg gtgactggag tttccggttc cggaaagtct tctttggttt acgaaatttt
     7861 gtataaaaat ttgcaaggtc gcctggaacg gcgttatcgg accaacgagg tttttaactg
     7921 caaggaattt tctggttcag aatatttagg gcgagtgatt atggttgacc aatcaccgat
     7981 tggccgcacc ccgcgttcca atccggccac ttataccggc gcctttactt ttattcgcaa
     8041 tttgtttgct tctactgcgt tggcgagagc gcggggctgg agtccgtctc gtttttcttt
     8101 taatgtccaa ggtggtcgtt gtgaagcctg tcaaggcaat ggggaaatcg cggtggaaat
     8161 gcactttctg ccgacgattt ttgtgccttg cgacgtttgc ggcggcaagc gttatgagaa
     8221 ggataccttg gaggtggagt acaagaagaa aaatatttac caagtgctca agatgacaat
     8281 tgaagaaaca ctcttgtttt ttaatgatat tccggctatt tatgaccgtt taaaaactct
     8341 taacgaggtt ggtttgggtt atttggaatt ggggcaacca gccaatactc tttccggcgg
     8401 cgaggcgcaa cgagttaaaa tttcgtccga actttatcgg ccacaaaccg aacggactat
     8461 ttatctttta gacgaaccga ctatcgggct acattatgaa gatgtaaaaa atttaataga
     8521 aattttgcaa aagttagtag aaaaggggaa cactgttttg gtcattgaac ataatatgga
     8581 agtgattaaa aatgccgatt acgtgattga tatggggccg gaaggcggca acggcggtgg
     8641 ccaggtggtc gctgccggca caccggaaga agtggcttac gccagtggct cgcacacggc
     8701 caaatatcta aaacgagcgt tgaacttgaa caagagagac aaaaaacagt agtattagca
     8761 aagagccaag ttgaccggct ctaagtcctt ttatgttttt acgtattgat atcacaaagt
     8821 ataagctgcc agacaaaccg ggcgtttatt attttttggg gtcgtcgaaa cttggcaaga
     8881 caacaaaaga tgcgcgtggg gttttgtata ttggcaaggc cacttctctc aaagatcggg
     8941 tgcgcagcta ttttaatggc gatcttggcg aaactcgtgg accaaagata gaacaaatgc
     9001 ttttgcaagc ggctgacctg aagtggcaag aaaccgattc agtgcttgag gccttgattc
     9061 tggaagccga gcttataaaa aaacatcaac cgccatataa cacccgcgaa aaagatgaca
     9121 agagttattg gtatgtggtt atcaccaaag aagattaccc gcgcgtcttg atgagacgcg
     9181 ggcgcgagtt agagaaagac ttgtcccgaa aatatgagat tgaaaaaact ttcggtccat
     9241 ttcctttttc gtcagagtta aaagcggccc taaaaattgt tcgccggatt ttcccttatc
     9301 gcgacacttg tttactttat tctgaaaaaa ataagggtct aaaagttccg ggtttgcgcg
     9361 gttgtttcaa tagccaaatt ggtctttgtc ccggagtttg cacgggcgcg attgacaaga
     9421 aaacttaccg tcaaataatc aagaatctga ggctcttttt tgagggcaaa aaagagcaag
     9481 tggtaaaaaa tttggaaaaa gaaatgaaaa tagcggtcaa agcgcaaaac tttgaggaag
     9541 ccgcgcgctg gcgcgatcaa atttttgcgc ttaagcatat ccaagatgtt tccttgctaa
     9601 aaaataccaa aacagaaaat tttttgtttg aggctggagc agaactagtt aatcgtcggg
     9661 acggaaaaga aaagacggtt tttcgtttag aggcttatga tatagcgcat ttggccgggg
     9721 ccggcacggt tggcgcgatg gctgtatggg aagggcgcga actaaataaa agtgaatata
     9781 gacttttcaa attgcgcggt cggagcgcca acaagagtga cgataccggc aacctggcgg
     9841 aaattttgcg gcgacgtttt acgcatcggg aatggaggct gccagatttg gttgtggttg
     9901 atggcgggca agcgcaattt aacgcggctc aaaaaacttt gaatcagctg aaccttagtc
     9961 tgcccttggt tgcggtcgtc aaagacgaaa agcaccgccc gcgggaaata ttgggcgaga
    10021 agacaatggc caagaaccac cggcgaaaaa ttttggcggt taatgccgat gtccaccgtt
    10081 ttgtgcttgc ttaccataga aaaattttac ggcagatgtt aaaataaaaa gaatggattc
    10141 ctatccaagt tcactttttg atttatttat tttattgtcc tctcctactt tttggttggt
    10201 ggcgcttctt atctttgtgg cggccgctgt tttgggctat tggtatcgcg gccgcagcgg
    10261 ccgagtccaa atgtccataa aggaagcaaa cctaaaaacc aggcaaatca aggagttgga
    10321 aaaaattaat cgtgaattgg aaaacaaaaa tcgcgaactt tacgccaaag aactagagtt
    10381 ggtgatggcc aacaagcgct tgcagtccct agaggcagcc aagtcaaaat ttatttcagt
    10441 gaccactcat caattacgga cgcctctggc cgccattaag tggacttttg atatggccgt
    10501 caagggtcag ctgggcaaag ttaatgaaga gttcaaaaag tttatggaaa agggcttcca
    10561 aagcaccgag agggttattt ccattgtgaa tgacttgcta aaaatagaca cgattgaaac
    10621 tggcaagtta gactttgttt tcaaaccaac gaatattgtt gatttaataa acaatgttat
    10681 aggcgagttt tgtaaccagg taaagactcg gcaattggaa attatttttg aaaaacccgc
    10741 taatcgggtg ccgttcgtag agattgatga aaacaaaatt agaatggttc tggaaaactt
    10801 aatagacaat gccataaaat acaatttgcc aaatgggaaa attttcatca agataagcga
    10861 cgctcgtctt aacagcgcgg aaagcagttt gcaaatatca attaccgact cgggcattgg
    10921 gatcccggac ggcgaagaag acaaaatgtt tcaaaagttt ttccgagcca gcaatgccat
    10981 caaacaagaa ccggatggca gcggtttggg gctttatatc actcgagaaa tcatagagaa
    11041 acatcatggc gcgatttggt ttgaaagggg caaagatgga ggtaccagtt tcactttcac
    11101 tttacctttg caccagaaaa aagtataatg gggagcaggg aaaaatcctt gatttacaag
    11161 ctttaaacat ctaattttat aaaatgacaa agaaaatatt gattgttgaa gatgatgagt
    11221 tccttcgttc tctaaatgcc aaacgtttgg aaggggaagg ttttgaggtt ttgatagcgg
    11281 ttgatggcaa cgaagccttg actaaattga ccgaaataaa accagacttg atttttttgg
    11341 atttactttt gcctaatgta gacggttttg aagttttgac taaaatcaag caagatgaaa
    11401 caaccaaaaa tattccggta attgtctttt ctaacttagg acaggctgag gatattgaaa
    11461 aagccaaaaa attgggcgcc gatgatttta tggtcaaagc caattttacg ttggacgacg
    11521 tcatcagcaa gattaaggag acactcaagt agttgattgg tttctaaatt actctaaagt
    11581 tgttgccata ctgcttttaa cttttaactt attttcttct tgtgttaaaa tataagcatg
    11641 tcttcatcct tgattagggt cggagttatg cgtggcggtc ttgggtctga atatgaagtc
    11701 tcgctcaaaa cgggcgcgac cgttttggag tatttgccgc gcgaaaaata tcaaccgcga
    11761 gatattttga ttaccaaaga tggccaatgg catatggatg gttttgggat agaaccggct
    11821 cgtttgcgaa acagtctgga tgtcgtcttt aatgccttgc atggcgagtt cggcgaagat
    11881 gggcaagttc aaaccttgct tgataacaca atgctgccct ataccggttc tggccgtttg
    11941 gcttcggcgc taggaatgaa caaggtggcc gccaaagaaa ttatctcgcg cgccgggctg
    12001 aaggttccgc gcggtgtcca cctcaagttt aaaccggaga ctaatgctga agcagttgct
    12061 tatgacgttt ttttaaaaat ttcaccgcct tggattgtta agccggttgg tcgcggttcc
    12121 tccgtcggcg tctttttggc aaagaccttt gatgacttgg ttgttgcggt ttcggaatgt
    12181 ttcaaaatca gtgaagtcat cttggtggaa gaatacatca gagggcgcga agcgacctgc
    12241 ggtgtggtgg acgattttcg cggacataaa acttacccgc tcttgccgat tgaaatcgcg
    12301 ccgccggctg gcaaaaagtt ttttgattat gaagccaagt attccggtca gtcaactgag
    12361 atttgccctg gtcagttttc agttggagaa aaaagagaac tgggcgagtt ggcggcggct
    12421 gttcataaac tgcttggtct taagcattac tcacgcactg acttcatcgt ttcgccacgc
    12481 ggaatctata ttttggaagt caattctttg cccggtttga cagccgaatc tttggttccc
    12541 aaatccttgg cggctcttgg cgtccccctg ccgcattttt tggaccatgt tgtttccctc
    12601 gctttaggca aaaaataaat tcaggcgggc caatcgcgct cagggaggcc cttggctggt
    12661 tagtttaaca tagagcttgt tacgtagcat gttacgtaac aagctacgta acagctccgc
    12721 gccccaagga ggttcgacct cctttgaggg caatttattt ggtggtgtta ttttagcatt
    12781 tgcgcgaacc tttttcgagc ggaaactgaa cgaatgaacc gtcccgccac cgcctcgcaa
    12841 agcctcggca accccgaaag aaaaaacact ccttgcattt ctgatttggc gggggtggat
    12901 tttttctaaa aaaggaaagg gtgtttttct ttcggggttc tgccttccaa gtattcgggc
    12961 aggtggcggg actaatgcag actcggtttt gccaaaacta ttttctgggg aaaaggattt
    13021 ggcaaaacat tcggctactc ttttttgaat tcggatgtcg caaatggaaa aggaggggtt
    13081 tggggaggaa ttttccattt gatcctcttt ttgtttgggg taaatttgtt ttcttttggc
    13141 ggggttcggg caggcacaga ttgagaaaag attaaattca tattagtata gttgtgaggg
    13201 ttagtttcaa cgctcatact tatactatca tactatggaa agcaaacaaa ataaagagat
    13261 tggggataaa ataaaagtct ttcgagcaaa gcaaggattg acacaagatg ctcttgcgag
    13321 aaagtgcgat attccctata caacactaac caagattgaa tcaaatgtta ttacaaaacc
    13381 gtcaatccaa acagtaacaa agattgccga agggttggga ataagtattg acgctttgat
    13441 taaataaaat tatgacaaaa attgaaaaat taaataaaac cgaagaatta ttgagtaggg
    13501 gtgtcgaaga aataatcgac agatctagcc ttgaaaataa actgagaagc gggaagcaac
    13561 ttcgtattaa gctcggcatt gatccaacaa gtccaaacat acacttaggc agatcaattc
    13621 cattacttaa attgagagat tttcaagaac taggtcataa aattatatta atcattggtg
    13681 attttactgg tttaattggt gatacttcag ataaggatag cgagcgaccg atgttgttgg
    13741 aggaaaccat taaagaaaat ttaaaaacat acattaaaca agcagctaaa atcatcgaca
    13801 tcaatacgac tgaaatttat cataacagca aatggcttgg cgagttaggt tacatggata
    13861 ttggtcgaca agcagatatt ttttctctta gtgaatttat ttctcgtgag aatattagcc
    13921 gacgtttaaa tgaagggaaa agagtgtctt tgcgagagtt attgtacccc ttaatgcaag
    13981 ggtatgattc agtagaggta agggctgatg ttgaaatagg tgggacggat caacgtttta
    14041 atcttttagc gggaagagat atgcagcggt tatacaatca aaaaccacaa gacattctca
    14101 ccaatcctat tatagaagga ttagatggaa gaaaaatgag ttctagtttt ggtaatactg
    14161 ttaatctatt tgacacaccc aatgatatgt ttggtaaaat aatgtcacta aaagatgact
    14221 ttatcattaa atattttacc ttacttacgc gagttgatat aaaatcaatt agtgaatacg
    14281 attcgagttt gaaggctggc atcaaccctc gcgatcttaa aatgaaactt gcgtttgaga
    14341 ttgtgcgaat gtactgctcg gaaaaagatg cgcgagttgc cgaagattat ttcattaaaa
    14401 cattctctaa aaaagaaact cctgtaaatc tgccaaaatt taaacccagt aaatatgaca
    14461 ttatatctat tttgattgaa aacaagctaa catcatcaag atcagatgcc agaagaacaa
    14521 ttgagcaagg aggcgtgaag attgacggcg tggttataaa agacattcac catgttgtat
    14581 cgtcaggttc gatattacag aagggtaaaa ttaaattcgt agaaataata tgaataaaaa
    14641 tgtaattaag atatgcaaaa gttttattaa agaaatactt gagaaagaag attggttccg
    14701 tcaattagtt aatgacaagg cttgcgacct tttgttgcta acaggaactg ctaccgaaca
    14761 aacagtgtca cttcctcttt cagaaattga gaacatggaa aaacaaattg acctgtggga
    14821 gaaagagtca atttccgaaa gaggaagtgt tgcccaaaac cttaaaacca aactttccga
    14881 aacgaaggag aagttagaca agctcgtttc gctctatctt gacggcgaca ttgaacgcga
    14941 aatatatcta cagagaaaag accttctcct tcgtcagaaa gcaaagtttg aagaaagttt
    15001 taaagatttt gggcaacaga gaaaaaattg ggtcgaaccc ttgcggagtt tcgttttgtc
    15061 tttgagagaa gccgttattt tggaaaaagg cgaaaactat ttcgcatgga aagagttttt
    15121 ccaaaaaatc ggctcgaacc ccgttctcaa agacaaaacg ctttcatgca attgggggga
    15181 actttgggaa ttcaccgctt ccgccgtagg cggaaagggt tttgaaaaaa ttgatagcgc
    15241 gattttggct tccgcaaaat cgctccgctt tcttaattcc gacaatgttt cgtttggtgg
    15301 tcattatacc aaaaagttcg gactcatttc cagcagaatc ctaatgacga atgaatcagc
    15361 cccaccaccg ctcgttccgc tcgcgtgtcc cgcaaaaaat attctccgct tttctaattt
    15421 ggcgcgcgac caattttttc ttctaaaagg atgagaacat tttttgcggg actctgccct
    15481 caattcttcg ggcaagtggt ggggcttcaa tgcggactcg gttttgccaa actatttttc
    15541 tggggatttt gatttggcaa aaccgagact gaatttcccg cgcggaggag gggtctgggg
    15601 aggaatccgc gcgggctttt tgattttcgg cgaaatttct ataaacaact aatttttgct
    15661 aaaataacag taacgtatga acaccgaaga catacaatta gtatctctgg ccaaacaaaa
    15721 ccccaaagag tacgaaaaac tgtacaccaa atactctgac aaagttttca actatttttg
    15781 gtatcgtacc ggccatgata aaccgctctc cgaagatttg atgcaggaga cttttctgcg
    15841 cgcttttcag cacttaaaac gtttcaataa tcgcggatat tcatacctta cctatctcat
    15901 cactatcgct cataacgtgt tggtggatca ttaccgcaaa ccgaagtcga ttccgttcga
    15961 ggcaatagaa gatatcccgt ttgaaatcac cgaagatttg gagagaaaat ctgatgccga
    16021 atccttgtgg cgagccattc aggacctacc acaatcaaac agagataagc tcttgatgaa
    16081 gtatcagcag gacatgtcgg tcagggacat tgccacagca actggaacta ccgagaatgc
    16141 agtaaaacta tctttatctc gaaccagaaa gaaactaaaa gatcatccat acgttaaaga
    16201 tatcgtcgga tttgccgata caaaacgcaa acagacgagg gcgaggtttt tagataaaaa
    16261 gtagacacct ttttatcttt tctacgttga atattcatga acgcagagcg tggtcatttt
    16321 tggtgtaaat atttatatgg caatgaatca caaagatttt gtaagacaaa ctgcaataac
    16381 tgttggggta gcattggtta ccaccatgtt gacgggggcg gggtttttaa catgggcaaa
    16441 acataatcca tcctcttttg ttcaattaat tactccgagc acgcgcccta atgaaaatgc
    16501 aacgtccacg gctttcctct atccgtcaga atcaattctg gttgatacgg taaaaaaagc
    16561 caatcctgca gtggtagcca ttacaatctc taagaatgtt ccagtatatg agagatacct
    16621 tgagagaatg ccaagtccct ttgaggactt ctttggggac gatttttttg gtggttttac
    16681 tattccgcaa ttgcgccaaa aaggaacgga gttacgcgag gttggaggtg gttctggatt
    16741 tctggtttcg gccgatggct acattgttac aaatcgtcat gtagtcagtg atgaaagcgc
    16801 ggaatatacg gtctttacta acgatggcaa aaaacatgaa gcaaaagtaa tagcgcgtga
    16861 cccggctctt gatttagcag ttattaaaat cagtggctct ggttttcacc acttatcatt
    16921 tggtgattcc gacaaactcg aaattggcca gacggtaatt gctataggca acgctctcgc
    16981 ggaatttaga aataccgtat cggttggtgt tgtgtccggt ctgtctcgct caatcaccgc
    17041 ttccgatcgc aggggcggat ttgaagctct tgatcaactg attcaaaccg atgccggtat
    17101 caatcccgga aattccggtg gtcctcttat taatctttct ggcgaagttg taggtgtgaa
    17161 tgtcgctgtt gctgacggcg cagaaaatat tggctttgct ctaagtgcga acagcgtaaa
    17221 aagtatcgta gagtcggtta aaactaccgg caagattgtt cggccgtatc ttggcgttcg
    17281 ctatatagaa attaatgagg agataaagga gaaaaataag ctttccgttg attatggtgt
    17341 tcttgtccag cgtggagtgg aagtaagcga tttggctgta attcctggtt caccggcgga
    17401 caaagcgggt atcgtcgaga acgacattat tcttgaaatt gatggaatta agttaacatc
    17461 tgatcaaagc ctcgccgcga tcatcaggac gaaaaaagtt ggcgataccg taaggcttaa
    17521 aataaatcat aagggacaag aaaaaatagt ggcggtgaag cttgaagaaa tgaaatgaca
    17581 ccttttgaga actgacacgt ttaacatggt gtagacggag tggtcgaccg tcgctaagca
    17641 gaagtaagca ttgtatgact ttagtgaagc agataatgga aggtgcttgg ctaaagaaat
    17701 tcattcgctc aatttctgat gccacttagc atctcctcaa tacaactgaa tagtgcgtaa
    17761 tatcctcgca aaaaagcgag cataagagaa cagagattct ctaaggtctg ggggaaccag
    17821 tttggcatcc ccagatcgtg tgtagagtaa aatgtcaaat cccttgtttg aggtcgaata
    17881 cagggggcat tactagaagt taatcgcata caaatatgaa aacagaaatt caaaagagag
    17941 ataacaaaaa tcaggctttg tctcgacgcg atgacttcgc gagccccttc tctctcatag
    18001 atagattctt cgacgattct gtgtgggatc cgttcaacat gatgacgccg agtctcttcc
    18061 gaagtcgtgg gctcggtaat gtctcaacgg cattccccaa ggttgacgtt gaagaaacgg
    18121 acaatgaaat caaagtaact gcaaatgttc ctggcattga tccgaaggac atcaatgtag
    18181 aggttgggga tgactacctc tcactatcgg gaaagattga taaggaaaat aaagacgaga
    18241 aaaatggtaa ggtctaccga tatgagagag agtacggaga gtttaggcga gagttctcct
    18301 tacctgctcg ggtgaataag gattcgatcg tggcaaaatc caagaacgga gtgcttatga
    18361 taactctccc gaagtcggaa gaagaaatga agaaacgcgt taagatagat gttgagtaaa
    18421 ctaaaagacc cgcagatgcg ggtcttttag ttttgtaaaa tttcgccgaa aatcaaaaag
    18481 cccgcgcgga ttcctcccca g
//
LOCUS       DAOOLO010000008        18111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_3204_length_18111_cov_6.058540, whole genome shotgun sequence.
ACCESSION   DAOOLO010000008 DAOOLO010000000
VERSION     DAOOLO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3204_length_18111_cov_6.058540"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLH96_00945"
     CDS             <1..461
                     /locus_tag="PLH96_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60373.1"
                     /translation="ASFPKINQDIRKAIEALVGEKSDDPNSWGIDLITSQTTAIVFNH
                     DLNAALTGLPEKTLEALAMVKTAEGEKEKRRLEGEGTAAAAKAMLDAEAAGLKKKADD
                     LGMSGEALVMYQMMQEGLQAGQVIISGSDGIANILRMGEAILGSKPKEKS"
     gene            580..1083
                     /locus_tag="PLH96_00950"
     CDS             580..1083
                     /locus_tag="PLH96_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60374.1"
                     /translation="MPAGAAPPPANAPGQPGSTPAPAQQSLGQKITSYVVAAVIIAAV
                     VFGLTWLGQPKAVVETPAAAAFKHGFEVKVAAGPWRKVEEVDWAKVKSTFTIPAKGNI
                     HCDQAVNAGKRLHYAIRQPDGVIIEMKPLGEQSPEDKKQEVTAPVGAVVQVTSLTGEE
                     LSLLIQY"
     gene            complement(1139..1393)
                     /locus_tag="PLH96_00955"
     CDS             complement(1139..1393)
                     /locus_tag="PLH96_00955"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60375.1"
                     /translation="MTLLRNIENHILNPLILLMFGLAMIFFLWGLVEYFWQAKDGKED
                     RSQAVRNITWGVIGMTIMVSVYGIMHFIVSTICGLAGQSC"
     gene            complement(1404..1775)
                     /locus_tag="PLH96_00960"
     CDS             complement(1404..1775)
                     /locus_tag="PLH96_00960"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60376.1"
                     /translation="MLKRMVKVTPWALSAAPFMVLAQTGLLQGNTSFVERLAGAAGDI
                     LQWLVVIFIALAVVFFLLGLLNFMRKEGAERADAKKDMLWGIIIIAVMVSVWGLVEFL
                     QDLVGVDSNVTPQTPSIPVVQ"
     gene            complement(1919..2263)
                     /locus_tag="PLH96_00965"
     CDS             complement(1919..2263)
                     /locus_tag="PLH96_00965"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60377.1"
                     /translation="MSLSFSQIINNFLTGIISPLIALLVGIALALFLWGLVRYLKSGL
                     GDKAELEKAKTMMVWGVVVLAVMVSVWGLVQVLQEMFFGDSVPNGPPDIPCFSGENCP
                     DSIPRTPFETDD"
     gene            complement(2279..2782)
                     /locus_tag="PLH96_00970"
     CDS             complement(2279..2782)
                     /locus_tag="PLH96_00970"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60378.1"
                     /translation="MNIIVLKSLFVSVSFLLVFTLGATQVCFAQAPNDIDLLINPAGG
                     GSLDTPMNPANGGNTELPMSPQSEGIFTNPLKDGIDSLPGLLKLILDIMVQIGTTVII
                     IMIIYAGFLYTTARGNQSQLDKAHRVIRGVLIGSAIILGAFVIAQVIKDTADDLMEGV
                     MIEQNIV"
     gene            complement(2793..3233)
                     /locus_tag="PLH96_00975"
     CDS             complement(2793..3233)
                     /locus_tag="PLH96_00975"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HPB60379.1"
                     /translation="MKKNWEKIRLGLVFAVCLVLWLLPAIILAEGEDSEFHLVVCGTG
                     AEGAMDCDFGELKDLVGKTFKYVLGYIILPVATVLLIWAGFKTILEAYQGKPATFWRQ
                     MLRNIIIGMALAIGAYALVKTFINLFFSDDAAFQSIIDSVFGNN"
     gene            complement(3235..4527)
                     /locus_tag="PLH96_00980"
     CDS             complement(3235..4527)
                     /locus_tag="PLH96_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB60380.1"
                     /translation="MEKNNTFQLILLFVFGAAAVVGILIFSGVIPGFKKESVGTAGTV
                     VVWGTASDANLNRGFAELSKKNEDKFSLVYVRKDPVTFETELTEALASGTGPDVIIAP
                     HEVIFRQRDRIYPFSAKYYPKRNFYDTFVDGARLFEVGEAYYALPIGVDPLVLYYNKD
                     IYNTNGLSSPPKTWVEFVNYQPRLTILDDNNNLKQSAAALGNFLNIDHAKDIFSLLLL
                     QAGNPIVALNNGVIKQTLADNLGYAVMPTQAALEFFVQFADPAKSTYSWNRSWPSSRD
                     SFLAGKLANYFGPASEYAYLKSKNPHLNFDVAEVPQQSLDRRQTFGRFYGLAIMRSSP
                     RLAAVFPAVYTLSASGEAENIAAALSLQPAKRMILAAGSADPYTQIFYEAALVARAWL
                     DFNSASSRRIFSEMIDNYSSGRLRADQAIKEAAGLLNR"
     gene            complement(4529..5059)
                     /locus_tag="PLH96_00985"
     CDS             complement(4529..5059)
                     /locus_tag="PLH96_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB60381.1"
                     /translation="MAFSEPQKNIEQLELKPGERVADLGCGVGAYSLAAAPLVGVDGK
                     VYAVDIQKDLLERLRQEADRQGWRNIEVVWGDVERVSGSGLADGLVDAVIMANVLFMT
                     KSVYTTVAEVKRILKGRGRLLVIDWSESFGGLGPQPGDVFDKNKAKQVFEGNGFTLLR
                     EFPAGDHHNGLVFQKS"
     gene            complement(5262..7754)
                     /gene="gyrA"
                     /locus_tag="PLH96_00990"
     CDS             complement(5262..7754)
                     /gene="gyrA"
                     /locus_tag="PLH96_00990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPB60382.1"
                     /translation="MAKDKEDKDSKKNKEKKSDAPIPERLSIMPIDIVSEMKESYLNY
                     SMSVIVGRALPDVRDGLKPVQRRILYSMHELGLTAAAKHRKSATVVGDVLGKYHPHGD
                     SACYDAMTRLAQDFSMRYPLVDGQGNFGSIDGDSPAAMRYTEAKMAKLSGEVLRDIEK
                     DTVDFIPNYDGTRREPVVLPSAVPGLLLNGAVGIAVGMATKIPPHNLRELADALIYLA
                     DNPDATTEDLLEFVKGPDFPTGGLAFNEQDIKHAYATGKGGVLTRGEAEIVETKAGAY
                     QIVVSSIPYQVNKAELIMKIADLVRDKKLEGIRDIRDESTKDIRIVVDLKSGAFPQKI
                     LNSLYKHTELETTFHYNLLTLVDGVPKLLPLEGILEEFLKHRQVVVVRRTKFDLKKAE
                     ERAHILEGLKRAIDHIDEIIKTIKKAKDTSEAHAALVKNFKFSEIQATAILEMKLQKL
                     AGLERKKIEDELKEKLALIKELKAILADPKKVTAIVKNELVEVRDKYGDDRKTKIVRG
                     GVKEIKTEDLVPEKEEVLTVTAGGYIKRTDPSEFRAQKRGGVGVVDVNMKEEDFVTIF
                     LTANTHDDLLFFTDRGKAYQLKMYEVPEGKRATRGKSVMNFLALTDDEKVTSVLAMPK
                     EYKNADFSLMLVTTSGIAKKVSAESFKDVRRSGLIAIKLADDDLLLAARAVHKGDEMI
                     ISSRSGQAIRFKETDIREMGRNAAGVRAMKLKKGDVIIAAGVVHKEMKDAEYLVISEN
                     GYGKKTKLKEYKVQKRGGSGIKTISVTKKTGALVAAKVVDEELAEMVAISKQGQVIRT
                     KLSEIPSLGRQTQGVRIMKLREGDQIASMTGL"
     gene            complement(7765..8076)
                     /locus_tag="PLH96_00995"
     CDS             complement(7765..8076)
                     /locus_tag="PLH96_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60383.1"
                     /translation="MSIKNYIANVKEKPEAKRKTLLLVWSGGLTFLVALVWFVNINYV
                     AYNRSLEEAEILAKAEAAAKTGQVEVVAQKEEGRWWELIKTNVAAVGEGFKVLTGQMD
                     E"
     gene            complement(8198..8815)
                     /locus_tag="PLH96_01000"
     CDS             complement(8198..8815)
                     /locus_tag="PLH96_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB60384.1"
                     /translation="MIISYHGKAFIKVQQGDLTAAFNPPSKDSKVKAVRFGADLAMIS
                     LNHEDFNGAEMVSYANKTPFVIDGPGEYEIGGNFVLGVPSAGPGGLINTIYTFTIDNI
                     HFCHLGALTETGLEGLKEEIGEVDVLCVPIGDNDTLKAKDAFKLANSLEPFIIIPILV
                     GNNGENLTTFLKEAGAGDVMPIEKLTLKRKDLDGKEGEVVVLKQT"
     gene            8957..10165
                     /locus_tag="PLH96_01005"
     CDS             8957..10165
                     /locus_tag="PLH96_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB60385.1"
                     /translation="MSKTQQDLSQTTDQEQISEDIEQEESDFKTKKSGEHRAMDEVLK
                     SSPLPPEEGALIEGKVIAKGKKSLYIDLPPFGTGIIFGREYLNAKDIIKKVSIGDMVT
                     AKVIETEGEDGYIELSLKEAKQAIIWSEAEKATKGKTSFDLEVKDANKGGLILEWKGI
                     GGFLPASQLKAEHYPRVPDGDKDSIMEELKKFVGQTLTVSIIGTDSKEGKLIFSEKNQ
                     DSKLKKEIIGKYTLGDVVDGEVTGAVDFGIFIKIQEGLEGLIHISEIDWSLVENPREL
                     FKVGDKVHAKIIDIKEDKISLSIKALKENPWQEAEKKYKRGEVVKGVVIKHNRHGALV
                     SLEEGVAGLVHISEFTNEEELRRALELGKTYTFTINVFEPKDQKMTLLFGSVPERITE
                     DKKKTEAKKD"
     gene            complement(10561..11166)
                     /gene="pth"
                     /locus_tag="PLH96_01010"
     CDS             complement(10561..11166)
                     /gene="pth"
                     /locus_tag="PLH96_01010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPB60386.1"
                     /translation="MINNVIMGLGNPGLEYAKSRHSVGRMVVDLLVDKLNGKEWTLNK
                     KAKSLESRIKIGKGKKQKEISLVKPETFMNKSGLAVAIYASAPAGRKKLVVVHDDVDL
                     PLGKIRFAFGRGSGGHRGLESIIKSLKSKDFVRLRVGISPSTPTGKVKKPASDKVVDF
                     VIGNFRSAEEKILKKVLAEATEALVYLAENGLDKTMNIYNR"
     gene            11228..11833
                     /gene="lepB"
                     /locus_tag="PLH96_01015"
     CDS             11228..11833
                     /gene="lepB"
                     /locus_tag="PLH96_01015"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB60387.1"
                     /translation="MRTLFFSKNTQPTDQEQKIETAPKEESLWDLIKFAFILIAIIVP
                     FRMFVAQPFIVNGASMDPTFHEGDYLIVDELSYFLREPKRGEVAIFSNPANPSQYFIK
                     RIIGLPGETVSVHGDRVEVKNDKGQLVLEEPYLGSSSNRDRTSTLGQDEYFVMGDNRE
                     VSYDSRMWGPLKEEMLKGRAFLRLFPPKVISYLPGNYEFEI"
     gene            11843..13153
                     /gene="hisS"
                     /locus_tag="PLH96_01020"
     CDS             11843..13153
                     /gene="hisS"
                     /locus_tag="PLH96_01020"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPB60388.1"
                     /translation="MKAAKKTKGKVSALQSVKGMRDLIGDQVFVFQGFCEKAAEIALY
                     YGFKPIETPILEKEDLFARGVGQDTDIVSKEMFTLKTKGGDRLVMRPEGTASVMRAYA
                     ENGMHSWPQPVMFYYSGPFFRHENPQRGRYRQFYQFGLEILGSPKSILDATIIKLTTT
                     ILEESGFNNYTLKINSIGDAECRGAYRRDLVNYYKKNLKGVCADCRERIKTNPLRLLD
                     CKNPMCQEIKKDAPESIASLCGPCKAHFKEVLEYLDAMNIPYEIDSTVVRGLDYYSRT
                     VFEVFVIDQNEDGEQTPLAIGGGGRYDYLAKSLGLKKEIPAVGVSIGVDRLVELPSYV
                     KHAPRVVKKPKVFFIQLSFDAKLRSFEVIEALRKAHIPLTHSISKDSLSAQLAIAEKM
                     NVPYTVILGQKEALEGSVIVRNMDTRSQDTIKINKLADFLKKIK"
     gene            13306..13527
                     /locus_tag="PLH96_01025"
     CDS             13306..13527
                     /locus_tag="PLH96_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60389.1"
                     /translation="METIEITKNNGESNASVIRRFTKRAQSSNIVRKARGRRYATRTK
                     SELKKKQDALKRMTKRAQYERLKKLGKLK"
     gene            13527..14000
                     /gene="ybeY"
                     /locus_tag="PLH96_01030"
     CDS             13527..14000
                     /gene="ybeY"
                     /locus_tag="PLH96_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPB60390.1"
                     /translation="MPSTDENFLITNLTKRNVPDIPFVALKNKILGKSWNVSLVFVTP
                     AKARALNQTYRRKDYYPNVLTFPLGKKEGEIFINLTRAENEAREYEHSPMIHIAFLFI
                     HGLLHLKGLSHSSKMESEEQKFLKLFFIDDKKSHHRLGYRHQLDQTRRLRTKKRR"
     gene            13918..15084
                     /gene="ftsA"
                     /locus_tag="PLH96_01035"
     CDS             13918..15084
                     /gene="ftsA"
                     /locus_tag="PLH96_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPB60391.1"
                     /translation="MTKNLITGLDIGTNWIRLVVCEQKNGDSVPQVLALVKRQSKGLR
                     RGYVINLEETTQSIREALDDAERIVRSRIRKVIVGVGGLSLGSKTAEGGVVASRPDSE
                     ITESDLGRAVEASEAALTDMANKQILHRIPLSFKLDGEKVLGRPEGLRGSKLEVKTLF
                     ITGGTQHLRDMVRAAEEAGLIVEDLVASPFAASLAVMTKVQKMAGCVLVNIGSQTTSI
                     AVFEEGLPLSIQVFAIGSTDITNDIALGFKIPIEEAEKVKKGEGEPVGTKKKLDEIIE
                     ARLSDIFEFIEMHLKKIKRSGLLPAGLIITGGGASIANIEELAKDYFRLPAKTAQTAI
                     ATNSKSQIRDSAWSVSFGLCLFGVVEQEGNSFGARVTLKNTQKKILAWLKEFMP"
     gene            15241..16440
                     /gene="ftsZ"
                     /locus_tag="PLH96_01040"
     CDS             15241..16440
                     /gene="ftsZ"
                     /locus_tag="PLH96_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB60392.1"
                     /translation="MPKINPEIESFARIKVVGVGGSGTNAVNHMVSSKVQGVEFIVMN
                     TDAQHLHHSPASKKLHIGKNLTRGLGTGMDPEIGRRAAEESLEDVQNMIKGADMVFIS
                     CGLGGGTGSGAAPVIAKVAKEQGALTIGVVTRPFFFEGKQRAAIAEKGIEELKKEVDA
                     IIAIPNDRLLGIISKDTTCTSAFAMCDEILRQAVEGISNIITMPGIINVDFADIRAIM
                     ANAGSALMGIGTASGENRAVEAAKGAINSPLLDISINGAKGVLFTIAGGTDLTMFEIQ
                     ETAKAITSSIDSEAKVIFGAIQDEKLKKNEIRVTVIACGFPEATLSRRDRAEASAGTL
                     TETKKEEPASGKDAKEKEKPEVTPAKAAERQASKPLVANRSEKTPEEEEEDDWSSIPA
                     FLRRAKK"
     gene            16595..17518
                     /locus_tag="PLH96_01045"
     CDS             16595..17518
                     /locus_tag="PLH96_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB60393.1"
                     /translation="MHDLIIIGGGPAGVAAGVYAARKKLKTLILTESLNGQSSVSPEI
                     QNWIGEITISGVDLAKKLESHLRSYESGDFVIKTKEQVSAIKTKAEGFEVVSTGGVYL
                     AKTVLIAAGANRRKLTIKGAAEFEQKGVTYCASCDGPMFAGQQVAVIGGGNAGFCSAA
                     QLLAYCDNVTLLESAASLRAEQVTVDSLTANPKFKVLLNVELLEIKGDKFVTGLIYRD
                     KETGKKNELPVTGIFVEIGFVPSTEAFRDLVEMTDCNAIKIDHRTGQTSREGIWAAGD
                     CTDTFYHQNNIAAGDAVKAVEDIYNYLQKKR"
     gene            complement(17556..>18111)
                     /locus_tag="PLH96_01050"
     CDS             complement(17556..>18111)
                     /locus_tag="PLH96_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPB60394.1"
                     /translation="ATALSLSALGFMIYLILDRLILFHSHCDETVEQCHNAGHQMRGP
                     VTVLILAVHSFIDGLAVGLALQVSVTTGLVMAVAVLTHNFSDGINVVGLLLKDGRERR
                     EAFKWLLPISLAPALGILATNFFSLPENYLGLVLALFGGFFLYIGAAELLPESHHGHS
                     TRWTTLATVLGMTILYLAIKLAGG"
BASE COUNT         5144 a         4522 c         3852 g         4593 t
ORIGIN      
        1 tagcaagttt cccgaaaatt aatcaagata tccggaaagc gattgaagcc ctcgttggcg
       61 agaaaagcga tgaccccaat agttggggca ttgaccttat cacctcccaa acgacggcca
      121 tcgtcttcaa tcacgacctc aatgccgcct tgacgggatt gccagagaaa accttagagg
      181 ctttggcaat ggtcaagacg gccgaaggcg aaaaggaaaa aagaaggctt gagggagagg
      241 gtaccgcggc cgccgcaaaa gcgatgcttg atgccgaagc ggctggcttg aaaaagaaag
      301 ccgatgacct tggaatgtct ggagaagccc tagtcatgta ccaaatgatg caggaaggac
      361 tccaggctgg ccaggtgatc atcagcggct ccgacggaat tgccaatatc ctgagaatgg
      421 gagaggcaat tctaggcagc aaaccaaagg agaaaagtta agatgtggat accgttgttc
      481 atactagcta ctttttctgt cttttggctg gcgcaaaaca tgccaaagag acagggatcc
      541 ccaatcgctc cgccctctcc ggctccggca cagaatcctg tgccggcggg ggccgcaccg
      601 ccaccggcca acgcgccagg acagccaggg tcaaccccgg cgccggccca gcaaagcctc
      661 ggtcagaaaa tcaccagtta tgtcgtcgcg gccgtgatta tcgcagcggt agtctttggt
      721 ctgacctggc taggccagcc aaaggcggtg gttgaaaccc ccgcggctgc ggccttcaaa
      781 catgggtttg aggtcaaagt cgcggccggc ccttggcgga aagtggagga ggttgactgg
      841 gctaaggtca agtcaacctt caccatcccc gccaagggga acattcactg cgaccaggca
      901 gtgaatgccg gtaagaggct tcactatgct atccgtcaac cagacggagt aatcatagag
      961 atgaagcctc tcggggagca gtccccggaa gacaagaaac aagaggtgac agcgccggtt
     1021 ggcgccgtcg ttcaagtcac ctctttaact ggcgaggagt tgtcactcct catccagtat
     1081 taaaatccaa ggcccgacgc gctagcgcgt cgggcccttt ctaattcttc ttttttttct
     1141 aacaagactg ccccgccaag ccgcaaatcg tgctcacaat aaaatgcata atcccataaa
     1201 ctgaaaccat aattgtcatt ccgattacgc cccaggtgat atttcgtaca gcttggcttc
     1261 tgtcttcttt tccatctttg gcttgccaaa aatattctac caagccccag agaaaaaata
     1321 tcatcgccaa accaaacatc agcaagatga gcggattcaa aatatggttt tctatgttgc
     1381 gcagcagggt cattttggtt taattactga acaaccggaa ttgaaggcgt ttgcggtgtc
     1441 acattactgt ctaccccaac caagtcctga agaaactcta caaggcccca aaccgaaacc
     1501 atcactgcga tgataattat accccacaac atatcttttt tagcatcagc tcgctcagcc
     1561 ccttctttcc gcataaaatt caaaagaccc aacaagaaaa aaacgactgc taaagcgata
     1621 aatataacca ctaaccattg aagaatatca cccgcggctc cagctaatct ttcaacaaaa
     1681 cttgtgtttc cttgcaacag acctgtttgc gccaaaacca taaacggcgc agccgataag
     1741 gcccaaggag tcacttttac cattctcttt aacataatat ttataattta ataattaaac
     1801 taataattga taatttttcg actgttttta atactttatt attataaaca cttttgattt
     1861 aaaaataaat aactggaata ttcaatttcc cccgccaaaa gaccgggatt caaaatcgct
     1921 aatcatcagt ttcaaacgga gtcctgggga tactatcagg acagttctcg ccggaaaagc
     1981 agggtatgtc cggcggaccg tttggcaccg aatcgccaaa aaacatttct tgcaaaacct
     2041 ggaccaaacc ccaaaccgaa accatcaccg ccagcaccac caccccccaa accatcatcg
     2101 tcttggcttt ttccagttcg gccttgtcac ccaggcccga cttgagatat ctgactaaac
     2161 cccacaaaaa gagcgctagg gcaatgccga ccaagagagc gattaaagga gaaattatcc
     2221 ccgttaaaaa attatttatg atttggctaa atgataacga cataagttat ttttttaatt
     2281 atacaatgtt ttgttcgatc attacaccct ccatcaagtc atcagcggta tcttttatga
     2341 cctgcgcaat cacaaacgcg cccagaatga tggccgagcc aatcaaaact ccgcgaatta
     2401 cccgatgagc tttatccaac tggctttgat tgcctctggc cgtcgtataa agaaagccgg
     2461 cgtagataat cataatgatt atcactgtcg taccaatctg gaccattata tccaaaatca
     2521 actttaagag tcctggcaag ctgtctatgc cgtctttaag ggggttggta aaaatacctt
     2581 cgctttgggg gctcattgga agttcagtgt ttccgccgtt agcgggattc atcggagtat
     2641 ctaaacttcc accaccagct ggattaatta acaagtctat gtcatttggg gcttgggcaa
     2701 agcaaacttg agttgctcca agcgtgaaaa caagcagaaa actaacagaa acaaaaagag
     2761 attttaagac gattatgttc atattaattg ttttaattat tgccaaaaac tgagtcaata
     2821 atagattgga aagcggcatc atcactaaag aacaggttaa tgaaggtctt caccagcgcg
     2881 taggcgccaa tcgccaacgc catgccaata attatattcc taagcatctg gcgccaaaaa
     2941 gtggcgggct tgccctgata tgcttccaaa atcgtcttga aaccggccca aattagcaaa
     3001 acagtcgcca ccggcaaaat aatatagccc aaaacatact taaaggtttt acccactaaa
     3061 tcttttagtt ctccaaaatc acaatccatg gcgccttccg cgccagtccc gcaaacaact
     3121 aggtgaaact ccgagtcttc gccttccgcc aaaattatgg ccggcaaaag ccacaagacc
     3181 aagcaaacgg caaacaccaa ccccaagcga attttttccc aatttttttt cattttatct
     3241 gtttaataac ccggcggctt ctttgattgc ctggtcggcg cgcaaacgcc cgcttgaata
     3301 attatcaatc atttcgctga aaattcgccg gctagacgct gaattaaaat caagccacgc
     3361 gcgcgcgacc aatgccgcct cgtaaaaaat ttgcgtgtag gggtcggccg agccggcagc
     3421 caaaatcatc cgcttggccg gctggagcga taaggccgcc gcgatattct cggcttcgcc
     3481 ggacgccgac aaagtataaa cagccggaaa aaccgccgcc aaacgcggag aacttctcat
     3541 tatcgccaaa ccataaaagc ggccaaaggt ctgacgtcgg tccaaacttt gttgcggcac
     3601 ttccgccacg tcaaaattga gatgcgggtt tttgcttttt aggtaagcat attcgctggc
     3661 tgggccaaaa taattggcca atttgcccgc caaaaaactg tcgcgggatg acggccaaga
     3721 ccgattccaa ctataagttg atttggccgg gtcggcaaat tgcacgaaaa attccaaagc
     3781 agcctgcgtc ggcatcactg catagcccaa attatccgct aaagtttgtt tgataacgcc
     3841 attgttcaaa gcgacaatcg gattgccggc ttgcaacaaa aggagggaaa aaatatcctt
     3901 ggcgtggtca atgttcaaaa aattgcccaa ggcggccgcg ctctgtttca gattattgtt
     3961 gtcgtctaaa attgtcagtc tgggctggta gttaacaaat tctacccagg ttttgggcgg
     4021 cgaagacaaa ccgttggtgt tgtagatatc tttgttgtaa taaagaacga gcgggtccac
     4081 gccgatcggc aaagcataat aggcctctcc aacttcaaac aatctggcgc cgtcaacaaa
     4141 agtgtcgtaa aaattccgct ttggataata cttagccgaa aacggataaa ttctatcacg
     4201 ctggcgaaag ataacttcgt gtggcgcgat aatcacgtca gggccagtac ccgaagccaa
     4261 agcttcggtc aattcagtct caaaagtcac cggatctttt ctaacataaa ccaacgaaaa
     4321 cttgtcttcg ttttttttgc taagctcagc aaaaccacgg tttagattag catcacttgc
     4381 cgtgccccat accactacgg tcccggcggt accaaccgac tcttttttga aaccgggaat
     4441 tacacccgaa aaaatcaaaa tgcccaccac ggcggccgcg ccaaagacaa acaagagaat
     4501 cagctgaaat gtgttatttt tttccatttt aacttttttg gaaaactaaa ccgttatggt
     4561 ggtcgccggc tggaaactct ctcagcaacg taaaaccgtt accttcaaaa acttgtttcg
     4621 ctttattttt atcaaaaaca tcacccggtt gcggacccag tccgccaaaa gactcactcc
     4681 aatcaataac caacaaacga ccgcggcctt ttaagattcg tttgacttcg gctaccgttg
     4741 tataaactga cttagtcata aaaagcacgt tggccataat caccgcgtca accaaaccat
     4801 cagccaaacc tgaaccggag actctttcta catcacccca aaccacctct atgtttcgcc
     4861 aaccttggcg atccgcctct tggcgcaagc gttccagcaa atctttttgg atatccacgg
     4921 cataaacctt gccgtccacc cccactaacg gcgccgccgc caaactgtaa gcgccaactc
     4981 cgcaacccaa atcggcgact cgttcgccgg gtttcagctc cagttgttca atattctttt
     5041 gcggttcgct aaaagccatt gattattctt cattctattt taacacttgg cagaaaactg
     5101 aacaaggaaa agagcaagaa aactgtgaat agacttttaa ccctgaaaaa acaaaaactg
     5161 ttgaataatt gtaaaattag aaaagtcggt cacaatcaac aaagaggcgt gaacgcccct
     5221 ctttgttgat ttcttttgtt ggctttggtt ttgcgctatc tctagagccc tgtcattgaa
     5281 gcaatctggt caccttcgcg cagtttcatt atccgcacgc cttgagtttg acgtccgaga
     5341 gaaggaattt ccgacagttt agttcgaatc acctgtcctt gtttggaaat cgccaccatt
     5401 tccgccaatt cttcgtctac cactttggcc gcgaccaacg caccggtttt tttggtcaca
     5461 ctaatggtct taatgccaga gccgccgcgt ttttggactt tgtattcctt taatttggtt
     5521 tttttgccgt aaccattttc gctgattacc aaatactcgg catccttcat ttctttatga
     5581 acaacccccg ccgctataat cacatcgccc tttttgagtt tcattgcccg caccccagcc
     5641 gcgtttcgcc ccatctcacg aatatcggtt tctttgaaac gaatagcttg accggagcga
     5701 gacgaaataa tcatctcatc acccttgtgg accgcgcgcg ccgccaacaa aaggtcgtca
     5761 tctgccaatt taatcgcaat caaaccgctg cgacgaacgt ctttgaaact ttcggctgaa
     5821 acttttttgg caataccgct agtcgtcacg agcattagcg aaaagtcggc attcttatat
     5881 tccttgggca tcgccaatac agaagttact ttttcatcat cggtcaaagc caagaaattc
     5941 atcactgatt tgccgcgagt tgcccgtttg ccttccggca cctcatacat ttttagttgg
     6001 taagctttac cccggtcagt gaagaacaaa aggtcgtcgt gcgtattggc cgtcaagaaa
     6061 atcgtcacaa aatcttcttc tttcatgttg acgtccacca ctccgacccc gccacgcttc
     6121 tgggcgcgaa actcagacgg atcggtgcgt ttgatgtagc caccggccgt gaccgtaagc
     6181 acttcttctt tttccggcac taaatcttca gtcttaattt ccttgacgcc acccctcaca
     6241 atcttggtct tgcggtcgtc gccatattta tcacgcactt ctaccaactc atttttaaca
     6301 atggcggtca cttttttcgg gtcagccaaa attgccttca gttctttgat taaagccaat
     6361 ttttctttca gctcatcctc aatcttttta cgctccaagc cggccaactt ctgcagtttc
     6421 atttccaaaa ttgccgtcgc ctggatttcg gaaaacttaa aattctttac caacgcggca
     6481 tgcgcttccg acgtgtcttt ggctttttta atcgttttaa taatctcgtc aatgtggtca
     6541 atcgcgcgtt ttaagccctc caaaatatgc gcgcgctctt ccgctttctt taaatcaaac
     6601 ttagtccgac gaaccaccac cacttggcga tgcttcaaaa attcttccaa aatgccttcc
     6661 aagggcagaa gttttggcac cccatccacc aaagtcaata aattgtaatg gaaggttgtt
     6721 tctaattcgg tgtgcttata tagagaattc aaaatctttt ggggaaaagc gccactcttt
     6781 aagtcaacga caatacgaat atctttggtt gattcatcac gaatgtccct aatcccctcc
     6841 agttttttgt cgcgcaccaa atcagcaatt ttcattatca attcggcctt attaacttga
     6901 tacggaatgg aactaacaac aatttgataa gcgccggcct tggtctcaac aatttcagcc
     6961 tcgccgcgcg taagcacccc acctttgccg gtcgcgtaag catgcttgat atcttgctcg
     7021 ttaaaagcca atcctccggt tgggaagtca ggacctttga cgaattccaa caaatcttca
     7081 gtagtggcgt ccgggttatc cgccaaataa atcaaggcat cagccaattc acgcaggtta
     7141 tgcggcggaa tcttggtcgc catcccaacg gcgattccaa ccgcgccatt cagaagcaaa
     7201 ccgggcaccg ccgagggcaa gaccacgggc tcacgcctgg taccgtcata gttggggatg
     7261 aagtcaactg tgtctttttc aatatcacgc aaaacttcgc cagaaagttt ggccatcttc
     7321 gcctcggtgt aacgcattgc cgccggcgag tcgccgtcaa tggaaccaaa gttgccctga
     7381 ccatctacca agggataacg catggaaaaa tcttgcgcca agcgggtcat tgcgtcatag
     7441 caagcgctgt cgccatgcgg atgatatttg cccaaaacat cgccaacgac tgttgctgac
     7501 ttgcggtgtt tcgccgcggc cgttagtcca agttcgtgca ttgaataaag gatgcgtcgc
     7561 tggaccggtt tcagaccgtc gcgaacatcc ggcaaagcgc ggccaacaat aacagacatt
     7621 gagtagttca aataggactc tttcatttct gacactatgt caatcggcat aatagaaagg
     7681 cgctccggaa tcggcgcatc gctctttttt tccttatttt ttttactgtc tttgtcttct
     7741 ttatcttttg ccatattttt tatcttactc gtccatttgt ccggtcaaga ccttgaaacc
     7801 ttctccgacc gccgcgacat ttgttttaat cagctcccac catctaccct cttctttttg
     7861 agcaactact tcaacttgtc cggttttggc tgccgcttcc gccttagcca atatttctgc
     7921 ttcttccaaa ctccgattat aagccacata atttatattg acaaaccaaa caagcgcgac
     7981 taaaaaagtc agtcctccac tccaaaccaa gagcaaagtt ttacgttttg cctccggctt
     8041 ctctttgaca ttggcaatat agtttttgat agacatggta ttaataatat aaaaattaaa
     8101 atgtaaaaat atggtcctaa taaataaacg tttgcctgac tgtctgcctt tatctgtcta
     8161 acgacagata aaggcagagg caaccaaaag aacgcgttta agtttgtttt aaaacaacaa
     8221 cctcaccttc tttgccatcc aaatcttttc ttttcagagt caacttctca attggcatta
     8281 cgtcgccggc gcccgcctct ttcaaaaaag tggtcaaatt ttctccatta ttaccgacca
     8341 aaatcggaat aataataaac ggctccaggg agttggccaa tttaaacgcg tctttggctt
     8401 ttaaagtgtc gttatcaccg atcggcacac acaagacatc aacttcacca atttcttcct
     8461 taagaccttc cagccctgtt tcagtcaaag cccccaagtg acaaaaatgg atattatcaa
     8521 tggtaaaagt ataaatggta ttaattaaac cgccgggacc ggccgaaggc acacccaaaa
     8581 caaaattacc accaatttca tattcacccg gaccgtcaat cacgaatgga gttttgttgg
     8641 cataggaaac catctcggcg ccattaaagt cttcgtggtt aagcgaaatc atcgccaaat
     8701 cagcgccaaa acgaaccgcc ttgaccttgg aatctttgct tggtggatta aaggcggcgg
     8761 ttaaatcacc ttgttgaacc tttataaatg ccttgccgtg atagctgata atcataattt
     8821 gttaatttca ttttagcatg tttacccaac gaacgccagt caatccaacg gggttttagc
     8881 aatgacaaac gcgaatatac aagtgagatg aatgaatttt tgactttttc tccaatttct
     8941 cttatacttt tctattatga gtaaaacaca acaagatctg tctcaaacga ctgatcaaga
     9001 gcaaatttcc gaggacatcg agcaagaaga gtccgatttt aaaacaaaaa aaagcgggga
     9061 acatcgcgcg atggacgagg tcttaaaaag ctctcctctc ccgccggaag aaggcgcact
     9121 tatagagggc aaggttatcg ccaaaggcaa aaaatcgctt tacatcgact tgccgccttt
     9181 tggcaccgga atcattttcg gccgtgagta tttgaatgcc aaagatatca tcaaaaaagt
     9241 atccatcggc gacatggtta ccgccaaagt aatcgagact gagggcgaag atggctatat
     9301 tgaactttct ctcaaagaag cgaaacaggc aatcatttgg agcgaagccg aaaaggcaac
     9361 caagggtaaa acttcgtttg acttggaagt taaagacgcc aacaagggtg gtttaatttt
     9421 ggaatggaaa ggaattggcg gcttcttgcc ggcctctcaa cttaaagccg aacattaccc
     9481 gcgcgtgcca gacggcgaca aagactccat catggaagaa ctaaaaaaat tcgtcggcca
     9541 gactttgacc gtttccatca tcggcaccga ctctaaagaa ggcaaactta ttttctctga
     9601 aaaaaaccaa gattctaaac tcaaaaaaga aataatcggc aagtacactc ttggcgatgt
     9661 tgtagacggc gaagtaaccg gcgctgtaga ttttggtatt tttatcaaaa ttcaagaagg
     9721 tttggaaggt ttaattcaca tctccgaaat tgactggtct ttggtagaaa atccgcgaga
     9781 actcttcaag gtaggtgata aggttcacgc caaaatcatt gatatcaaag aagacaaaat
     9841 ttctctgtct atcaaagctt taaaagaaaa tccctggcaa gaagccgaaa agaaatataa
     9901 gcgaggtgaa gtggtcaaag gtgttgtcat caaacataac cgacacggcg ccttggtcag
     9961 ccttgaagaa ggcgtcgccg gcttagtcca catttccgag tttacaaacg aagaggaatt
    10021 acgccgcgcg ctggaattag gcaaaaccta tacctttaca atcaatgttt ttgaaccaaa
    10081 agaccaaaaa atgactcttt tatttggttc ggtgccggaa aggataaccg aggacaagaa
    10141 aaaaactgaa gcgaaaaaag attaacaaga aattcaaaaa aacaagacct tctcccaaag
    10201 agagggcctt gttttttttg aatttggccc aaaatttttg tgttctaaaa tctttcatga
    10261 accaatcccc ccaatcatca ggaaagaaag ttggggtcgg actcccgccc aagacaaaat
    10321 tttgcgttat ccacaaaaca agtccaagcc gacttgactt ttgtttattt tcttgatacc
    10381 ctaaaagagg ttaagtgttg gcgggcaacc tttcgtcaga cgaacctcct tacacattcc
    10441 acaataacct cgagggagtt gcccgccacg ccaacaacca gctcagtttg agctggttga
    10501 gatagattag gcccgtcctc gtgacgggcc atttttttat ttttaaactt ttttatttga
    10561 ttatctgtta tagatattca tcgtcttatc caggccgttt tccgccagat acactaacgc
    10621 ttcagttgct tcagccaaaa ctttttttag aatcttttct tccgccgacc taaaattacc
    10681 aatcacaaaa tcaaccactt tgtcacttgc cggcttttta accttgcctg ttggagtaga
    10741 cggtgaaata ccaactcgca gcctgacaaa atctttggat ttgagtgatt tgatgattga
    10801 ttccagaccg cgatgcccgc ccgaaccgcg gccaaaagca aatctgattt tgcccaatgg
    10861 caaatccaca tcgtcatgaa caaccaccaa ttttttgcgc ccagccggcg cgctagcgta
    10921 aattgccacc gccaaaccgg atttgttcat aaaagtttcc ggcttgacca atgagatttc
    10981 tttttgcttt ttgcccttgc caatcttaat cctgctctcc aacgactttg cttttttgtt
    11041 gagcgtccat tctttgccgt tcagcttgtc aaccaacaaa tcaactacca ttcgcccgac
    11101 gctgtgacgg cttttggcat attccaaacc cggattaccc aagcccataa taacgttatt
    11161 tatcataaaa caattatacc ggcacctctt tcttgcgtca aagcggtatt tgctataata
    11221 tttgaatatg agaacgcttt tcttcagtaa aaacactcag cctaccgacc aagaacaaaa
    11281 aattgagacc gcgcccaagg aggaaagtct ttgggacctt ataaaatttg cgttcatctt
    11341 gattgccatc atcgtgcctt ttcgcatgtt tgtcgctcaa cccttcattg tgaacggcgc
    11401 ctcgatggac ccaacttttc atgaaggtga ctacttaatc gtcgacgaat tgtcttattt
    11461 tttgcgagaa ccaaaacgcg gcgaggtcgc catctttagc aaccctgcca acccctcgca
    11521 atattttatc aaacgcataa tcggtctgcc gggcgagacg gtcagcgtcc acggtgaccg
    11581 cgtggaagtg aaaaatgaca agggtcaact tgtcttggaa gaaccatatc ttggctccag
    11641 tagcaaccgc gaccgaacat caactctcgg ccaagatgaa tactttgtga tgggcgacaa
    11701 ccgcgaagtc agttatgact cgcgaatgtg gggtcctctt aaagaagaga tgttaaaggg
    11761 tcgcgctttt ttgcgtctct ttccgccaaa agtaatttcc tatttacccg gcaattacga
    11821 gtttgaaata taaaattaaa atatgaaagc cgccaaaaaa actaagggca aggttagcgc
    11881 cttgcagtca gtcaaaggta tgcgtgattt aatcggcgac caagttttcg tttttcaagg
    11941 tttttgcgag aaagcagccg agattgccct ttattatggt tttaagccaa tcgaaacacc
    12001 gattctggaa aaagaagatt tgttcgcgcg cggcgttggc caagacaccg acattgtcag
    12061 caaagagatg ttcaccctaa aaactaaagg gggcgaccgt ttggtaatgc ggccagaagg
    12121 cacggccagc gtcatgcgcg cctacgcaga gaatggaatg cattcttggc ctcaaccggt
    12181 aatgttttat tacagtggac cttttttccg acacgaaaat ccgcagcgcg gccgataccg
    12241 ccaattctat caatttggtt tggaaatttt gggctccccc aaaagcattt tagacgccac
    12301 catcatcaaa ctaacgacca ctatcctgga agaatccggt tttaataatt acaccctgaa
    12361 gataaacagc attggcgatg ctgagtgccg aggcgcttac cgccgagact tggttaatta
    12421 ctacaaaaaa aatctaaagg gagtttgcgc tgattgtcgt gaacgaatca aaacaaaccc
    12481 gctccgtttg cttgattgca agaatccgat gtgccaagaa attaaaaagg acgccccgga
    12541 gtctattgct tcgctctgcg gcccgtgcaa ggcgcacttc aaagaagttt tggagtattt
    12601 agacgcgatg aacattcctt atgaaataga cagcacggtg gtgcgcggcc tggactatta
    12661 ctcgcgcaca gtgtttgagg tttttgttat tgaccaaaat gaagatggcg aacaaacccc
    12721 gctagcgatc ggcggcggcg gtcgttatga ctatctggcc aaaagcttgg gccttaaaaa
    12781 agaaattccg gcggtcggtg tttcaattgg tgttgaccgc ttggtggagc taccgtctta
    12841 tgttaagcac gcgccgcgag ttgtcaaaaa accgaaagtt tttttcatcc aattgtcgtt
    12901 tgacgccaag ttgcgcagct ttgaagtgat tgaggccttg cgtaaagctc acatcccgct
    12961 gacccattcc atctccaagg attctctctc ggctcaactg gcaatcgccg aaaaaatgaa
    13021 tgttccctac acggttatcc tcggccaaaa agaagccctt gaaggctccg tgattgtgcg
    13081 aaatatggat acccgctcac aagatacaat taaaataaat aagttggccg acttcctcaa
    13141 aaaaatcaag tagcttggag tttgaaagcg gccagccccg tccgccaatc ctgctgattc
    13201 aggaggttca ggaagtccga cctccttttt cttgtttcag gtcagcgttt tatccgcatt
    13261 aaacttttga ctttaatgcc cccctgtgct atcctgtcta agtttatgga aactatagaa
    13321 atcaccaaaa ataacggcga atcaaatgcc agtgttatta gacgctttac caaacgcgct
    13381 caatcttcca atatcgtccg taaagcccgt ggccgtcgtt atgccacccg caccaagtca
    13441 gaacttaaga aaaagcaaga tgccttaaaa cgcatgacca aacgcgctca atatgaacgt
    13501 ctgaagaaac ttggcaaact gaagtaatgc cctccactga tgaaaatttt ctaatcacga
    13561 atctaaccaa aaggaatgtc cccgacattc cttttgtcgc tctaaaaaat aaaatcttgg
    13621 gaaaaagctg gaatgttagc ctggtttttg ttacgcccgc caaagcccgc gcccttaatc
    13681 aaacctatcg ccgaaaagat tattacccaa acgtgctcac ctttccgctt ggcaaaaaag
    13741 aaggtgaaat tttcataaac ttaacgcgcg ccgagaatga agcgcgtgaa tatgagcact
    13801 cacctatgat tcatatcgct tttttgttta tccacggcct tttgcatttg aaaggtttgt
    13861 cacatagtag taaaatggag agtgaagagc aaaaattttt aaaattattt tttattgatg
    13921 acaaaaaatc tcatcaccgg cttggatatc ggcaccaact ggatcagact cgtcgtttgc
    13981 gaacaaaaaa acggcgatag cgttccgcaa gttttggcat tggtcaaaag acaatccaag
    14041 ggcctgcgcc gcggttatgt catcaatctg gaagaaacaa ctcaatcaat tcgtgaagcg
    14101 ttggatgatg ccgaacgcat tgtccgtagt cgtatccgta aagttattgt cggagttggc
    14161 ggtctgtcgt tagggtcaaa aaccgccgaa ggcggggtgg tcgcctctcg ccccgattcg
    14221 gaaataaccg agagcgacct ggggcgagcg gtagaggcca gcgaagcggc gttgaccgat
    14281 atggccaaca aacaaattct ccaccgcatt cccctgtctt ttaaacttga tggcgaaaag
    14341 gtcttgggcc ggccggaggg tttgcgcggt tccaaactgg aagtcaaaac tttgtttatc
    14401 accggcggca cccaacacct gcgcgacatg gtccgcgccg cggaagaagc cggtttgatt
    14461 gtagaagatt tggttgcctc gccttttgcc gcttcgctcg ccgtcatgac caaggttcaa
    14521 aagatggccg gttgcgtcct ggtcaatatt ggttcgcaaa ccacctccat tgcagtcttt
    14581 gaggaaggct tgccgctttc tattcaggtt tttgccattg gttctaccga catcaccaac
    14641 gatatcgctc tcggttttaa aatcccgatt gaagaagcgg agaaagtaaa aaaaggtgag
    14701 ggcgaaccgg ttggcactaa aaagaaattg gacgaaatta ttgaagcgcg cctttcagac
    14761 atttttgaat ttatagaaat gcacttgaaa aaaatcaagc ggagcgggct tttacccgcc
    14821 ggtctgatta tcaccggtgg cggcgccagt attgcgaaca ttgaggaact ggccaaggat
    14881 tattttcgcc tgccggccaa gacggcccaa acagccattg ccaccaattc caaaagccag
    14941 attcgcgact cagcttggtc ggtctctttc ggtttgtgtc tttttggcgt tgtcgaacaa
    15001 gaaggaaact cttttggcgc gcgtgtcact ctaaaaaaca ctcaaaagaa aattttggct
    15061 tggttaaaag agtttatgcc ctagcaagaa acaacgtctc tctaatgacg aaaacagcta
    15121 aaagctgttt ttattgatga aaatcacccc ccttgggggc tttgcctttt tcgctttatt
    15181 tgttaagatt aaattgttca aaattgttta aataaagtta ttacttttac ttaccaaaat
    15241 atgcccaaaa ttaaccctga gattgaatct tttgcccgaa tcaaagttgt cggcgtcggc
    15301 ggctccggca caaacgctgt caatcatatg gtcagctcca aagtccaagg tgtggaattt
    15361 atcgtgatga acactgacgc gcagcacctt caccattccc cagccagcaa aaaactacat
    15421 attggcaaaa acctaacccg cggtttgggc accggcatgg acccggagat aggacgccgc
    15481 gccgccgagg agagtctgga agatgtccaa aatatgatta agggcgccga tatggttttc
    15541 atttcctgtg gtcttggcgg aggaaccggt tccggcgccg cgccagttat cgccaaagtc
    15601 gccaaggagc aaggcgcgct caccattggc gttgttaccc gtccattctt ctttgaaggc
    15661 aaacaacgcg ccgccattgc cgaaaaaggc attgaggaac ttaaaaaaga agttgacgcg
    15721 attattgcca tccccaatga ccgtcttttg ggcatcatca gcaaagacac cacctgcact
    15781 tcagcttttg caatgtgcga tgaaattctc cgccaagcgg tagagggcat ttccaacatc
    15841 atcacgatgc ccggcatcat caatgtggac tttgccgata tccgagccat tatggccaac
    15901 gccggttcag ccttgatggg tatcggcacc gcttctggtg aaaatcgagc cgttgaagcc
    15961 gccaaggggg ccattaattc cccgctgctg gacatttcca tcaatggggc caaaggcgtc
    16021 ctcttcacta ttgccggcgg cactgacttg accatgtttg aaatccaaga aacggccaag
    16081 gccattactt cgtctattga ttcagaggcc aaagttattt ttggcgccat ccaagacgaa
    16141 aaattgaaga aaaatgaaat cagagtaacc gttatcgctt gcggtttccc agaagcaacg
    16201 cttagccgtc gtgaccgcgc tgaagctagc gccggtactc tgacagaaac aaaaaaggaa
    16261 gagccagcgt ccggcaaaga tgccaaagaa aaagaaaagc ccgaggttac tccagccaaa
    16321 gccgccgaac ggcaggcctc aaaaccactg gtagccaatc ggtctgaaaa aacaccagaa
    16381 gaagaggagg aggacgattg gtcgtcaatt ccggctttct taaggagagc aaaaaaatag
    16441 agaggtagaa tttgaaatat agaatagagg gcggtaaaga atagaaaatt tggatagcta
    16501 atatctaggt gtattcctta tttaatttaa aacttccgcc ccccaatttt ccgtatttga
    16561 gttttacatt ttaattttta cactttaaat tatcatgcat gatctaatca ttatcggtgg
    16621 cggaccggct ggtgtagccg ccggtgtcta tgcggccaga aaaaagttaa aaaccttgat
    16681 cctgaccgaa agcctaaatg gccagtctag tgtttcgccg gaaattcaaa actggatcgg
    16741 cgaaattacc atctccggcg ttgatttggc caaaaagtta gagtcgcatt tgcgttctta
    16801 tgaaagcggt gattttgtaa tcaaaaccaa agaacaagtt tccgcgatta aaactaaagc
    16861 agaagggttt gaagttgttt ccaccggggg cgtttatctg gccaaaacag tcttaattgc
    16921 cgccggtgcc aaccgccgca aactgacaat caaaggcgcg gccgaatttg aacaaaaagg
    16981 tgtcacctac tgcgcttctt gcgacggacc aatgtttgcc ggtcaacaag tggccgttat
    17041 cggcggcggc aacgcgggct tttgttcggc cgctcagctc ctcgcctatt gcgacaacgt
    17101 tactttgtta gaaagcgcgg cctcgctccg cgccgagcaa gttaccgtgg atagtctaac
    17161 agccaacccc aaatttaagg ttctgctcaa cgtggagttg ctagaaatca aaggcgacaa
    17221 atttgttacc ggtcttatct atcgtgacaa agaaacaggc aaaaaaaacg aactgccggt
    17281 aaccggtatt tttgtggaaa tcggatttgt gcccagcacc gaggctttcc gcgatctggt
    17341 ggaaatgacc gattgcaacg cgattaaaat tgaccaccgc accggccaaa cctcccgcga
    17401 gggaatctgg gccgccggtg actgcaccga cacgttttat catcaaaaca acatcgccgc
    17461 tggcgatgcc gtcaaggccg tggaggatat ttataattat ttgcagaaaa aaagataaga
    17521 gtttctcggt tctgtcggtt ctaggcggtg ttttattagc cacctgctaa ttttatcgcc
    17581 agataaagaa tcgtcatccc caagacagta gccaaagtgg tccaacgcgt ggaatgccca
    17641 tggtggcttt ccggcaaaag ttcagccgcg ccgatataaa gaaagaaacc gccaaacagc
    17701 gccagaacaa gaccaagata attttccggc aaactaaaga aattggtggc caaaatgccc
    17761 aacgccggcg ccaaagatat cggcaaaagc cacttgaacg cctcccgacg ttcccgacca
    17821 tcttttaaca agagaccaac aacgttgatc ccatcagaaa agttatgagt caaaaccgct
    17881 acggccatca ccagaccagt ggtcactgat acttgcagcg ccagcccaac cgccaaaccg
    17941 tcgataaaac tgtgaacagc caaaatcaaa accgtcaccg gaccgcgcat ttggtggccg
    18001 gcgttatgac attgttcaac cgtttcgtca caatgcgaat gaaagagaat caacctgtcc
    18061 aaaatcaaat aaatcataaa acccaacgcg ctaagggaaa gagcggttgc c
//
LOCUS       DAOOLO010000009        17904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_3260_length_17904_cov_5.956524, whole genome shotgun sequence.
ACCESSION   DAOOLO010000009 DAOOLO010000000
VERSION     DAOOLO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17904
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_17904_cov_5.956524"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /gene="tsaD"
                     /locus_tag="PLH96_01055"
     CDS             <1..855
                     /gene="tsaD"
                     /locus_tag="PLH96_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB60395.1"
                     /translation="PRIDAIAVTKGPRLEPALWVGINFARALSLVWRKPLIPVNHLEG
                     HLCAGLVKEKGKDKKTNPKNPPLNFPALALIVSGGHTELVLAHDWLKYKQIGGTRDDA
                     VGEAFDKVARILGLPYPGGPQISKLAKETKNRKENPISLPRPMINSPDFDFSFSGLKT
                     AVLYLVQKLKAENKLDKETKSFVAADFQQACLDVLLAKTQKALIKFKPRTVIVGGGVI
                     ANRELRQQLTRMITRNFPTVSLVIPELGYTTDNATMIAAAGYLQSLSKKKLRVAPKLR
                     AQGQMPLK"
     gene            882..3002
                     /locus_tag="PLH96_01060"
     CDS             882..3002
                     /locus_tag="PLH96_01060"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPB60396.1"
                     /translation="MNSKFTKPYNPTENESVIYELWEKSGYFNPDNLPNVTEGPFTII
                     MPPPNANGQIHAGHALFLTLEDIMIRYKRMRGFKTLWLPGTDHAGFETQVVYEKKLEK
                     EGRSRFNLSREELYAEIMDFVQANKKATENGMRQLGASCDWSREKFTLDPDIIQTVYA
                     TFKQMSDDGLVYRGERITNWCTKHQTSLSDLETLSETRKDKLYYMQYGPLVVATVRPE
                     TMFGDVAVAVNPDDERYRQYIGQDILVQTPIGELKLKVIADETVEKDFGTGALKITPA
                     HDANDFEIGLRHNLPSVEVIDRYGKLNEKTGKYAGLKIEAAREAVVRDLAELGLLLKT
                     EDYEHAVSVCYKCGRVIEPRIIPQWFVKMKNLAEQAVKAVKDKKIQIIPNHQEKVFCH
                     WLDNIRDWNISRQIAWGIPIPAKLCEKCDYAVVDIENQKTDCPKCGGRLIKDPDTFDT
                     WFSSGQWPYATLGYPTGEDYRNFYPTDVMETAGDIIFFWVARMVMFGLYRTGQVPFQT
                     VYLHGLVRDAKNQKMSKSKGNVIDPAVIARKYGTDALRMALVTGNAAGTDMALSEDRI
                     KGYKHFANKIWNITRFILENSQGQNSPVDLVPADEKLLAEFRALTADITTDMENFRFH
                     LASEKIYQYTWHTLADVILEESKPLIFGNNESNRLSRLLTLNIILLDLLKLLHPFMPF
                     LTETIWQSLPQTKEKMLLTARWPE"
     gene            3099..3878
                     /locus_tag="PLH96_01065"
     CDS             3099..3878
                     /locus_tag="PLH96_01065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPB60397.1"
                     /translation="MKALDGWGGHSILFAVLFGLAPTIFWLWFWLQEDEDKPEPARMI
                     LKTFVVGGFSVVIAFCLERLVAFNPDIAGNFQSSLSVAGIKAALITLWPILAWAFIEE
                     VVKYAAAYIAAFRKQHFDEPVDAMVYMITAALGFAAVENFLFLFSTALSSGANSSSFV
                     FTGNLRFLGATLLHVVTSATLGAFIGFSFYKNSNIKKGSLGLGLVAATVLHTLFNFFI
                     IIDEGKNIFGVLVFLWIITTVIIFVFEVVKKVGAKSLPSNL"
     gene            3898..4377
                     /locus_tag="PLH96_01070"
     CDS             3898..4377
                     /locus_tag="PLH96_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB60398.1"
                     /translation="MFKEKRSFFERITGSISVNDEEDVVTVPEIKDDGNFAVSAKTND
                     WPEEEPEGELSVDVYQTDSEIILQSMIAGVKPENLTISITREMITLQGKRERPSDAAN
                     DDYFLRELYWGSFSRKVLLPAEVEPTECEAFEKNGLLTIRMPKIDKEKTQKIKVRSL"
     assembly_gap    4385..4484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4492..4564)
                     /locus_tag="PLH96_01075"
     tRNA            complement(4492..4564)
                     /locus_tag="PLH96_01075"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4529..4531),aa:Phe,seq:gaa)
     gene            complement(4614..4871)
                     /locus_tag="PLH96_01080"
     CDS             complement(4614..4871)
                     /locus_tag="PLH96_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60399.1"
                     /translation="MDDETRELIKKSVELGEANRRALDKLLRYQKNVQIFAALKLMVI
                     VLSAVGAFYYLQPFWERLWSTYNSLVESFSTVNIRGGGFPL"
     gene            complement(5030..6327)
                     /locus_tag="PLH96_01085"
                     /pseudo
     CDS             complement(5030..6327)
                     /locus_tag="PLH96_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
     gene            complement(6365..6436)
                     /locus_tag="PLH96_01090"
     tRNA            complement(6365..6436)
                     /locus_tag="PLH96_01090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6401..6403),aa:Arg,seq:cct)
     gene            complement(6495..6731)
                     /locus_tag="PLH96_01095"
     CDS             complement(6495..6731)
                     /locus_tag="PLH96_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HPB60400.1"
                     /translation="MNKKVAVYTSPTCHFCHLAKDFLTEKGVSFEEYDVLANVEKRQE
                     MIEKSGQMGVPVIVVEDKIMVGFDEQELSKLLGL"
     gene            complement(6777..7070)
                     /locus_tag="PLH96_01100"
     CDS             complement(6777..7070)
                     /locus_tag="PLH96_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HPB60401.1"
                     /translation="MSSKFQRTIEDFSCAHCGFFVPGDGFTNHCPKCLWSRHVDNYPG
                     DRANDCLGLMEPTGLELEKGQYVIVHECQKCRTRKRVKARNEDDIRKFLQSML"
     gene            complement(7325..7395)
                     /locus_tag="PLH96_01105"
     tRNA            complement(7325..7395)
                     /locus_tag="PLH96_01105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7361..7363),aa:Gly,seq:ccc)
     gene            complement(7498..7857)
                     /locus_tag="PLH96_01110"
     CDS             complement(7498..7857)
                     /locus_tag="PLH96_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPB60402.1"
                     /translation="MAAIQGKKKKRRYLIGLPMAILAVLVLLIFVRAAWKMYQRDALS
                     RDNLKMAEEQLAALKAREADLQTKIKALETPRGIEEEIRANFSVAKEGEKVISLVEEE
                     ATTTATTTEKKPWWRLF"
     gene            complement(8032..9828)
                     /gene="lepA"
                     /locus_tag="PLH96_01115"
     CDS             complement(8032..9828)
                     /gene="lepA"
                     /locus_tag="PLH96_01115"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPB60403.1"
                     /translation="MDLKHIRNFSIIAHIDHGKSTLSDRLLEVTGAIPKRQMKEQVLD
                     TMELERERGITIKMQPVRMKYDYQGENYELNLIDTPGHIDFSYEVSRALKAVDGVILL
                     VDATQGVQAQTFTVLAMAQELGRVIIPVINKIDLPVARVEETKEEVVKLLGCRPEEIL
                     AVSGKTGAGAAELLAKIIEVVPPPQSTYSLEQGSRSLVFDFEYSKHRGIIVYVRVFDG
                     EIKKGDQLVFSVAAEKFTAQEVGVFAPAKIAQSSLSAGEIGYIVTGIKERGQAKVGET
                     VVKAGKPLPAFAGYMLPHPVVWASIYPESQDDFGLLKQALERLNLQDAALSFEEEYSG
                     SLGRGFRAGFLGMLHLEIITERLRREFGLTLITTMPTISYEVLDKKSSQTEIAYSPQL
                     FPKETKDKVIREPWIKVKIITPSSCLGAIIPLLHEHEAIITVTENFGGDRTSLDIEMP
                     LRELMRNFFDELKSVTSGFASLSYEIGEMRESDVVRLDVLLNGEVMPAFSRIVSRRRA
                     EREAEDLADKLKELLPKQLIVIKIQVEGMGRIIAARSISALRKDVTGYLYGGDITRKM
                     KLLEKQKKGKKKMQKIGKVDVPHEVFLKMMKS"
     gene            complement(9971..11659)
                     /locus_tag="PLH96_01120"
     CDS             complement(9971..11659)
                     /locus_tag="PLH96_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPB60404.1"
                     /translation="MVNKVIKLFRREVAGLHEAAILLGLFALTSQFLGLIRDRLLAGS
                     LGAGTALDIYYSSFRIPDLIFATIASFVSVTVLIPLLSQKISEGRIAEARNFMAGIFS
                     SFLIVMAIISSLVFLLMPFLAEWLAPGFDQEARQEMILLSRILLLSPILLGLSNLYGS
                     ITQAYKKFLLYSLCPVFYNLGIIVGIVFFLPRLGLSGIVWGVILGSSFHFLLQVPSII
                     EFEFWPRLSLTAIKQNFEDIKKVVLISLPRTITLSINQIALFFVVALASVLGQGAISI
                     FNLSFNLQSVPSVIFGVSYSVAAFPTLSNLFYKKQMKEFLGKIREASQHIIFWSLPAT
                     ALFIVLRAQIVRVILGSGEFGWTATRLTAACLALFSISVVTQGLVHLFVRAYYAGGLT
                     KKPLFINVLSSLLAIVSAFGLVYLYSRVDFLRYVVEKLLRVENLEGSIILMLPLAFSL
                     GSIVNVVLMLWMFKKDFNFELRPLADTFIRALLASLLGGAVSYQLLNWLVRFFDLNTF
                     VGVFAQGFLAGLGGIVAMFVALWFLRSQELKHLMVSLRHKFWKVDVVRPEPEEL"
     gene            complement(11661..>12765)
                     /locus_tag="PLH96_01125"
     CDS             complement(11661..>12765)
                     /locus_tag="PLH96_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB60405.1"
                     /translation="ALAGSIYLWVFSVIYVPDSFLLALTFFVTGLFISQLTENKIIPS
                     LEIWLNRDSKLNLGAVLLILVLVIGLSGSGFYLVQKYVSLYFYQKGQVELIYNKNLDR
                     AQDLVGRAIKLSAQDLYYRKASEIDLAKISNTLTQNDLGMEQKQKQFETLFNSAVANT
                     QKALEIDKKNYLNWLAQGNVYQLAVSLGVEGSYQKALDSYLQAQKLSPTNPSILVDNL
                     ARLEIQKGDSQKAKDYLNQAKALKPDYYPAVLLETQLDLDSGDSVSGLKKLEDFAVAY
                     PQAADQTFYFQLGYLKFIQLDYAGAIRAFSQAVALEPDFANAKYFRALSYEALGQRSE
                     ALKEFVDIQKTNPDSDEVKQAIQRLKGEPSLGE"
     assembly_gap    12766..12865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12866..13782)
                     /locus_tag="PLH96_01130"
     CDS             complement(<12866..13782)
                     /locus_tag="PLH96_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60406.1"
                     /translation="MEKISPWPFAKMSLVIVALCLFALPWFNFSVWGASFEVGKKLLL
                     AVAALVALVFWLISQLQENKIILPKNCILLTLGGVLTLSFLSAVFSGNFWRSIVGFGF
                     EQDTFFTLFILLTILFLAASLFQSKRRFLSIYSVLFISALVLFVLQAIVVLVFKLDFF
                     AFGRPFFSFFSNGSISSLVGKWYDFGVYYGFVAISSLFFLEFRSERKSLPSRLAASCL
                     GISLVALVFVNYLPVWVALLVFSLWLFFYKVFLVKKGSLQSKTLAGFSFLVVMVSIFF
                     LTLGSVEGLGQSWASGRTKISGVAIFDVKP"
     gene            13871..14083
                     /locus_tag="PLH96_01135"
     CDS             13871..14083
                     /locus_tag="PLH96_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB60407.1"
                     /translation="MEKGLVTIKSAAEILGVSVETLRNWDRRGVLKAKRSPQNGYRLY
                     NISELQKMLKQKQIKEPRVKRAKLAD"
     gene            complement(14892..17246)
                     /locus_tag="PLH96_01140"
     CDS             complement(14892..17246)
                     /locus_tag="PLH96_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HPB60408.1"
                     /translation="MAKNIKTAQKTSKKIGTKVSKVTEVKKRDGTVVPFDESKITNAV
                     LKAMKAVAEGGEKEATKVAAAVTTLISNTKKEQGKDYLPTVEEIQDLVEKELILQKYS
                     STAKAYILYREKRAEIRKQKGEVPREVKNLVTESKKYFRNQLSEFVYYTTYSRWIPEK
                     GRRETWMETVDRYVNFMRDNLGGKLTKKEYDEIRHNMLSMRAMGSMRLLWAAGKAAAA
                     THVCAYNCSYIAPTDWRDFGEIMYVLMCGTGCGYSVEHQNVEQLPIIQRQTGEKLKTH
                     VIKDSKEGWADAFVLGLRTWAAGKDIAFDYSEIRPQGARLKTMGGRASGPEPLRNLID
                     FSRAKMLTRQGRRLTTLDVHDIICKTGEVVVMGGVRRSALISLSDLDDQEMKEAKNGQ
                     FYISHPERMMANNSVVYNEKPTIAEFLDEWLNLIKAQSGERGTFNRGSLEKQLPARRW
                     PIFKKDAQYSGLNPCGEIVLKSKQFCNLSEVVARREDTLADLADKVRVATILGTYQST
                     LTKFGYLSPEWRKNCEEERLLGVSITGQWDCPALRNPEAMKKLQKVAVETNKKYAKRF
                     GIAQSTAVTCVKPSGNGSQLFDSSSGCHPRHAKYYIRRVRIERHNPIFLMLKDMGVPY
                     HPEVGQTDANANTYVLEFPVKAPEGAVVKKDITAMDQLEYWKMLKENYTEHNPSVTVS
                     VGEDEWLRVGNWLYENWDLVGGLSFLPRNDYVYKLAPYEEITKERYEMMAKDFPEIDF
                     SKIVLYEYDDVTTGSKELACAGGTCEIDPLAGGLSTVDTKEEKK"
     gene            complement(17471..>17904)
                     /locus_tag="PLH96_01145"
     CDS             complement(17471..>17904)
                     /locus_tag="PLH96_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60409.1"
                     /translation="VNFLASVSFEQRVEFKGGMDIKRGIMIKDKGTGEEYCIFLRDGE
                     EVTEKGNCDQILGETTAAATTTLPTAIPLEMNNGTLMSTSTEATEMVTVESSDPMTAT
                     TSSDIVPEVNEAAVIAEVREEEEIETVGTGDSGVAESLEAN"
BASE COUNT         5139 a         4358 c         3484 g         4723 t
ORIGIN      
        1 cctaggattg acgctatcgc cgtgaccaaa ggtcctcgct tggaaccagc cctttgggtg
       61 ggtatcaatt ttgcccgcgc gctgtctctg gtctggcgta aaccgctaat ccccgtcaac
      121 cacctggaag gacatctctg cgccggcttg gtaaaagaaa aaggtaaaga caaaaaaaca
      181 aatcccaaaa atcctcctct caattttccg gccttggcat tgattgtttc cggcggtcac
      241 actgaattag ttttggctca tgactggctc aaatataaac aaatcggcgg cacgcgcgat
      301 gacgcggtcg gcgaagcttt tgataaagtg gctcgaattc ttggattgcc ctaccccgga
      361 ggaccgcaaa tttccaaact ggccaaagaa acaaaaaacc gaaaagaaaa tccgatttct
      421 ctgcctcgcc caatgattaa ttcgccggat tttgatttct ctttttccgg tcttaaaacc
      481 gctgttcttt acctggtcca aaaactgaag gccgagaaca aattagacaa ggaaacaaaa
      541 tctttcgtcg cggctgattt tcaacaggct tgccttgatg ttttgttggc caaaacccaa
      601 aaagccctta ttaaatttaa accaaggacc gtaattgtgg gcggcggcgt catcgccaac
      661 cgcgaactaa gacaacaatt gacaagaatg attacccgaa atttcccaac tgtctccttg
      721 gtaatccccg agttaggtta cactaccgac aacgccacca tgattgctgc cgccggttac
      781 ttgcaaagtt tgagcaagaa aaaactgagg gtggcgccaa agctccgcgc tcaaggccaa
      841 atgcctttga agtaaaaggt ttttttgtta ttatactatt gatgaactcc aaatttacca
      901 aaccctacaa cccgaccgaa aacgaatcgg tgatttatga attatgggaa aaaagcggct
      961 acttcaatcc cgataatttg ccaaatgtga ccgagggccc attcacgatt attatgccgc
     1021 cgccaaacgc caacggtcaa attcacgccg gccacgcgtt gtttctcacg ttggaagaca
     1081 taatgattcg ctacaaacga atgaggggct ttaaaacact ctggctgccc ggaactgacc
     1141 atgccggatt tgaaacacaa gtcgtctatg agaaaaagtt ggaaaaagaa ggccgttctc
     1201 gtttcaatct tagccgtgaa gaactttatg ccgaaattat ggactttgtt caagccaaca
     1261 aaaaagcgac cgagaatggc atgcgccagc taggcgcctc ctgtgattgg tctcgtgaaa
     1321 aattcactct tgaccctgat atcattcaaa cagtctatgc tacttttaaa cagatgtctg
     1381 acgacggttt ggtttatcgc ggggagagaa taaccaactg gtgtaccaaa catcaaactt
     1441 ccctctctga cctagaaacc ttgagcgaaa ctaggaaaga taaactttat tatatgcaat
     1501 acggaccact agtcgtcgcg acagttcgac cggaaacaat gtttggagat gtcgcggtgg
     1561 cggtcaatcc ggacgatgaa cgctatcgcc aatacatcgg ccaagacatc ttagttcaaa
     1621 caccaattgg cgaactaaaa ttaaaagtta tcgctgacga aacagtggaa aaagattttg
     1681 gcaccggcgc gttgaaaatt acccccgccc acgatgccaa tgactttgaa attggtctgc
     1741 gtcataattt accttccgtt gaagtaatag accgttacgg taagctaaat gaaaaaaccg
     1801 gcaaatatgc cggccttaaa atagaagcgg cgcgcgaagc agtggttaga gacttggctg
     1861 aactggggct cctcttaaaa accgaagatt acgaacatgc tgtctcggtt tgctataaat
     1921 gcggacgtgt aatagaaccg cgaattattc cgcaatggtt tgtcaagatg aaaaatttag
     1981 ccgaacaggc ggtaaaagcg gtaaaggata aaaaaatcca aattatcccc aatcaccaag
     2041 aaaaagtttt ctgtcattgg cttgacaata ttcgcgactg gaacatttcg cggcaaatcg
     2101 cctggggaat tcccatccct gccaaattat gtgaaaaatg cgactacgcc gtggtagata
     2161 ttgaaaacca aaaaaccgat tgcccaaaat gcggaggtcg gctaatcaaa gacccggaca
     2221 cttttgacac ttggttttcc tccggacaat ggccctatgc cactctgggc tacccaactg
     2281 gcgaagacta ccggaatttt tatccgaccg atgtgatgga aacagctggc gatattattt
     2341 ttttctgggt tgctcgcatg gtaatgtttg ggctttatcg gaccggccaa gttcctttcc
     2401 aaactgttta tttacatggc ttagtccgcg acgccaaaaa tcaaaaaatg tccaagagca
     2461 aaggcaacgt gattgacccg gctgtcattg ccagaaaata cggcactgac gccctcagaa
     2521 tggccttagt gacgggaaac gccgctggaa ccgacatggc cctgtctgaa gacagaatca
     2581 aaggttataa acactttgcc aacaaaatct ggaatattac ccgcttcatt ctggaaaatt
     2641 cccagggcca aaactcgccg gtcgacctgg taccagctga cgagaagttg ttggccgaat
     2701 tccgcgcgct gaccgctgat attaccaccg atatggaaaa ctttcgtttt catctcgcca
     2761 gcgaaaaaat ctaccaatac acctggcaca ctttggccga cgtcattttg gaagaaagta
     2821 aaccgctaat tttcggcaat aatgagtcga accgtctttc ccgcctcttg actctaaata
     2881 tcattttgct ggatttgcta aagctcttgc acccctttat gccgttcttg accgaaacca
     2941 tctggcaaag cctccctcaa accaaagaaa aaatgcttct gaccgcccgc tggccggaat
     3001 aattttaaaa tcaccagtta tcatctgtca atttaaatta attgacaagt gacaacaaac
     3061 aaaaaccaat gagcttcacc gattacataa aaatttttat gaaagcgctt gacggttggg
     3121 gtggccattc cattttattt gccgttctct ttggtttggc gccaacaatt ttttggctct
     3181 ggttttggct tcaagaagac gaggacaagc cggaaccggc ccggatgatt ctgaaaactt
     3241 ttgtggttgg tggcttttct gttgttattg ctttttgcct agaacgactg gttgctttca
     3301 atcccgacat agcggggaac tttcaatctt ctctttccgt ggccggaatc aaggctgctc
     3361 tgattactct ttggccaatt ctggcttggg cttttattga ggaggtggtc aaatatgccg
     3421 ccgcttacat cgctgctttt cgcaaacaac actttgatga accggttgac gccatggttt
     3481 atatgataac ggccgccctt ggtttcgccg ccgtagaaaa ttttttgttt ttgttttcta
     3541 ccgccttaag cagtggggcc aacagcagtt cctttgtctt caccggcaac ctgcgtttct
     3601 tgggagcaac ccttctccat gtcgttacct cagccacgct cggcgctttc atcggctttt
     3661 cattttataa gaactcaaat ataaaaaaag ggtctctcgg tttggggtta gtggccgcca
     3721 ctgtcttgca tactctcttc aactttttta taattataga tgaggggaaa aatatttttg
     3781 gggttttggt ttttctttgg ataattacca ccgttattat ttttgttttt gaagtagtta
     3841 aaaaagttgg cgctaaaagc ttgccatcaa acttataaat aatcactaac ctaatctatg
     3901 tttaaagaaa aaagatcttt ttttgaaaga attaccggca gtatttcggt caatgacgaa
     3961 gaggacgtcg tgacagtccc cgaaataaaa gacgatggca actttgcggt cagcgccaaa
     4021 accaacgact ggccggaaga agaaccggaa ggcgagctgt cggttgatgt ttatcaaacc
     4081 gacagtgaaa taattcttca atcaatgatt gctggcgtca aaccggagaa cctgaccatt
     4141 tccattacgc gcgaaatgat tactctccag ggcaaaagag aaagaccctc agatgccgcc
     4201 aatgatgact acttcttgcg cgaactttat tggggttcct tttcgcgcaa agttttattg
     4261 ccagccgaag tggagccgac cgaatgtgaa gcttttgaga aaaatggcct cttaactatc
     4321 cgtatgccca aaatagacaa ggaaaagacc caaaagatta aggtgcggtc gttgtaggaa
     4381 ctagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctttgt gtgctcgggg
     4501 ccgggcttga accggcgacc tatcgctctt caggcgagcg ctctaccaac tgagctaccc
     4561 gagcatattt tatttaagaa tagagtattt tggccaaaaa atcaacttga cttttatagc
     4621 gggaaaccgc cgcctctgat gttcacagtg ctgaaacttt caaccaaact gttgtaggtt
     4681 gaccacaatc tttcccaaaa aggctggagg tagtaaaacg cgccaacagc tgacaaaaca
     4741 atcaccatca acttcagcgc ggcaaaaatt tgcacatttt tctggtaacg caataacttg
     4801 tctagggctc gtctattggc ctcaccaagc tccacgcttt ttttaataag ttctcttgtc
     4861 tcatcatcca tagttaaatt atacaatgat ttttaacaaa agacatcaga aaagcccgtt
     4921 caaatttgtt caaatctatt atcttatatt acaccacaaa acccctctta ggggagctac
     4981 ccagctcccc taagaggggt tttgtggtgt agtttagttt ttgtggtttt tatttcttgc
     5041 tatttttgtt agcgtctttt tcagcctcgc cccgtaagta tttctttccc ttcaatcgtt
     5101 ctggtaaata ttcctgctcc tcttgatagt caaaatcggg gctatatttg taacccttac
     5161 tataacccaa gtctttcatc agttttgtcg gtgcgttacg aatatgaagt gggactggtt
     5221 cattgattgt ctcacggaca tcacgcttta tttgattata agcctcataa agttcgttgc
     5281 tctttttaca cttggccaaa taaaccacca cttgcgccaa taccacttcg cattccggca
     5341 tcccgatata atgacaagct tggtaaccgg ccaccgcttg aaccaaggcc aaagaattgg
     5401 caaggccaac atcttcgctc gcaaatcgaa tcaagcgacg agcgacatag agcggatctt
     5461 cgcccccctc caacattcgc cccagccagt acagagccgc gtcggcatcg ctgccacgca
     5521 ttgatttgtg caaagccgaa ataatattgt aatgctcctc gcctcctttg tcatacatca
     5581 gataagactt ttgcagtgat tctttaatta aatctttttc aatcacaatt tcaccctttt
     5641 tgtttttggc cctgttttcg ccgccagttc caaagcattt aaggcattgc gcgcgtcacc
     5701 attggcgacg gttgccaaaa aatccaaagc ttcgtccgaa attttaaccg tcaaagcgcc
     5761 caaacctttc tttttgtccg tcagcgccct ttgtaaaatc agcttaatcg cctcttcgtc
     5821 taattgtttc aagacaaaaa cccgacaacg cgataaaagc gccgaattga cttcaaaact
     5881 cggattttcg gtcgtagcgc caatcaaggt cactgtgcca tcctccacgt gtggtaacaa
     5941 cgcgtcctgt tgggatttgc tccaacggtg tatctcatca ataaataaaa tggttcgttt
     6001 gccctctttc aagcgcgcgg ccgcctcttt aataactgcc cgcaactcgg ccaccccaga
     6061 agtaaccgct gaaaaagcga cgaaacctgc gccggacatt ctggcaataa ttttagccag
     6121 cgtcgttttg cctgaaccgg gcggccccca aaaaattaaa gaaggggttt catcttgctc
     6181 tatcagttta cgcaaaagct tgccttcgcc cacaatatcc gcttgaccga caaaatccgc
     6241 taaattttcc ggcctcaggc gatcggccaa aggggccatt ttgtcatctt tatctttgct
     6301 atttttgctt ttgtctattc ctatcataag ttaagcttaa aacaatttta tgaaataggg
     6361 ggtgtgcctc cagcaggaat cgaacctaca tctagggctt aggagtccct tgttctatcc
     6421 attgaactat gaaggcaaac taaaagggga tttggtcccc ttttagtttg ccacaaatcc
     6481 caacaaattt aaacctagag accaagcaac ttggacaatt cttgttcgtc aaaaccaacc
     6541 ataatcttgt cctccacgac aattaccggg acccccattt ggccgctttt ctctatcatt
     6601 tcctgtctct tttcaacgtt agccaaaaca tcatactcct caaaagacac ccctttttcg
     6661 gtcaaaaaat ctttggccaa gtgacagaaa tggcaagtgg gagaagtgta gaccgcgact
     6721 ttcttattca taatttaaaa caaataaaaa ttagctgata agagcttatt ttttaattat
     6781 aacatactct gtaagaattt cctaatatcg tcttcattac gcgccttcac ccgtttgcgt
     6841 gtccggcatt tttgacattc atggacaata acatattggc ccttttccaa ttctagccca
     6901 gtcggctcca tcaaacccaa acaatcgttg gcgcgatcac ccgggtaatt gtccacgtgc
     6961 ctggaccaaa gacacttagg gcaatgattg gtgaagccgt cacctggcac aaaaaaaccg
     7021 cagtgagcgc agctaaagtc ttcaattgta cgctgaaatt tggaagacat gggtcaatta
     7081 aaaatataaa attaaaatag aaaaccaaat gacttgtgcg agcacccacg ccaatgttgt
     7141 cgttgttagt aaaccagtcc ggaataccat gtgcgggcac ccacacctat tttctcgtga
     7201 cgaaagcaaa ttattttata atgaggtaac ttatatttta taaaactttc taaatttaca
     7261 ggcggtaccg tccgtcagat aacaagtctg actggttatc tgacaacgag aaaataggtg
     7321 tgggtgcggg tagggagaat cgaactcccg tcttagcctt gggaaggcca cattctgcca
     7381 ctaaaccata cccgcaatat aaaaatttta gtttaaaatt taaaagccaa aagttaaata
     7441 aagatccagt aaactacact ttgcgttcct gcgttagtct gcgtaaacct gtattcttca
     7501 gaataaacgc caccacggtt tcttttctgt cgttgtcgct gtcgtggtcg cttcttcctc
     7561 cactaaactt atcacttttt caccctcttt ggcaaccgaa aagttagccc tgatttcttc
     7621 ctctatcccg cgcggcgttt ccagcgcctt aatcttggtc tgtaaatcag cctcgcgcgc
     7681 cttgagggcg gccaattgct cctcggccat tttaagatta tcacggctta aagcgtctcg
     7741 ttggtacatt ttccaagcgg cccggacaaa aatcaacaac accaagactg ccaaaatcgc
     7801 catcggcagg ccaattaagt atcgcctctt cttttttttg ccttgaattg ccgccatttt
     7861 tggattataa tgtaactaat aacttaaatt atattatgtt tttaggaaac aaacaaaaga
     7921 aagttgttag agtcgcggcc gcggtaatcg gaatcctgac tattgtcagt atggttgctc
     7981 tatactccct gtcttatttc ttctagattc tctcagatcc tatttctacg tttacgactt
     8041 catcatcttc aaaaacactt cgtgcgggac atccaccttg ccgatttttt gcattttctt
     8101 tttgcctttc ttctgtttct ctaacagctt cattttacgc gtaatatccc cgccataaag
     8161 atagcccgta acgtcttttc gcaaggccga gatgctacga gccgcgataa ttcgccccat
     8221 tccctctact tgaattttta tcacaatcaa ctgtttgggc aaaagctcct tcagcttgtc
     8281 cgccaagtct tccgcttctc gttcagcccg gcggcgagac acaatccgtg aaaaagctgg
     8341 catcacttcg ccgtttagca acacgtctaa ccgaaccaca tccgattcac gcatctcgcc
     8401 aatttcataa gacaaagaag caaaaccgga agtgacactt ttaagttcat caaaaaagtt
     8461 acgcatcaat tcccgtaaag gcatttctat atccagcgag gtgcggtccc cgccaaaatt
     8521 ttccgtcacc gttataatcg cttcgtgttc gtgcaaaagc ggaattattg ctccaagaca
     8581 agaggacggc gtgatgattt ttacttttat ccaaggctct ctgatgacct tatccttggt
     8641 ttctttcggg aaaagttgcg gtgaataagc gatctcggtt tggctgcttt ttttgtctaa
     8701 aacttcataa ctaattgtag gcatcgtcgt aatcaaagtt aagccgaatt cacgtcgcag
     8761 acgctccgtg attatttcca agtgcaacat ccctaagaag ccagcgcgga aaccacggcc
     8821 aagcgagcca gagtactctt cctcaaagga aagggccgca tcctgcaaat ttaagcgttc
     8881 tagggcctgt ttgagcaagc caaagtcatc ctgactctcc ggataaatag aggcccaaac
     8941 cactggatgc ggcaacatat agccggcaaa ggccggtaaa ggcttaccag ctttaaccac
     9001 cgtctcaccc actttggcct ggccgcgttc tttgatgccg gttacaatgt aaccgatttc
     9061 accggccgac aggctacttt gagcaatttt agcaggcgcg aaaaccccca cttcttgagc
     9121 tgtaaatttt tctgccgcca ccgaaaaaac gagctggtcg ccttttttta tttcaccgtc
     9181 aaaaacgcga acataaacaa taatcccccg atgcttggaa tattcaaagt caaaaaccaa
     9241 tgaacgcgaa ccctgttcaa gagaataggt tgattgcggc ggcggcacaa cttctataat
     9301 tttagccaac aattcagccg cgccggcccc ggtcttgccg gaaaccgcca aaatctcttc
     9361 cggccgacaa ccaagaagtt tgactacctc ctcttttgtt tcttccactc tggccaccgg
     9421 caaatctatt ttgttgataa ccggaattat tactcgcccc agctcttgag ccatcgccag
     9481 gacagtaaaa gtttgcgctt ggaccccttg ggtcgcgtct accagtaaaa ttaccccgtc
     9541 caccgctttc agggcccgcg aaacttcata agaaaaatca atatgtcccg gcgtatcaat
     9601 taggttcaat tcataatttt cgccctggta atcatacttc attcgaactg gttgcatttt
     9661 gatggtaatc cctcgctcac gctccagctc catcgtatcc aaaacttgct ctttcatctg
     9721 acgtttggga atcgcgccag tcacttccaa aagccgatca gacaaggtgg acttgccgtg
     9781 gtcaatatgg gctattatag aaaaatttct aatgtgtttt aaatccattt tagtacagac
     9841 atcctatcgt aaaacagata tttattcaaa attttgctca cgcaaccaaa cgacaacggc
     9901 cagacaatgt tcactctttt ttcttccggc ccagttgtct tttctgttaa aaacaatctc
     9961 catgtttctc ttataactct tccggttctg gtctgacaac atcaaccttc caaaacttgt
    10021 gccgcaagga aaccatcaaa tgttttagct cttggctacg caaaaaccaa agcgcgacaa
    10081 acatcgccac gataccaccc agaccggcca aaaacccctg cgcaaaaact cccacaaagg
    10141 tgttgaggtc aaaaaagcga accaaccaat ttaaaagttg ataggacacc gcgccgccca
    10201 acaaagaagc caacaaagcc ctaataaaag tgtccgccag aggcctaagt tcaaaattaa
    10261 aatccttctt aaacatccac aacatcaaga caacgtttac gatggaacca agcgaaaaag
    10321 ccagcggtaa cattaaaata atgctgccct ccagattttc cacgcgcaat aatttttcca
    10381 caacataacg caaaaaatcc actcgcgagt aaagataaac caaaccgaaa gccgagacaa
    10441 tcgccagaag agaagagaga acattaataa acaacggctt cttggtcaaa ccgccggcat
    10501 aataagcgcg cacaaacaaa tgaaccaagc cctgagtcac gactgaaatg gaaaaaagcg
    10561 caagacaagc ggccgtcaaa cgagtcgcgg tccagccgaa ctccccgctc cctaaaataa
    10621 ccctcacaat ttgcgcccgc aaaacaataa acaaggcagt cgccggcaag gaccaaaaaa
    10681 taatgtgctg actagcctct ctgattttgc ccaaaaattc tttcatttgt tttttgtaaa
    10741 acaaattaga cagagtcggg aaagccgcca ccgaatagct gactccaaag ataactgagg
    10801 ggaccgactg taaattaaaa gacaggttaa aaattgagat cgcgccctgc cccaaaacgg
    10861 aggccaaagc aacaacaaaa aacaacgcga tttgattaat agaaagagtg atcgtgcgag
    10921 gcaaagaaat caaaaccact tttttaatat cttcaaagtt ttgctttatg gccgttaggg
    10981 acaaacgtgg ccagaattca aactctatga ttgatggaac ttggagcaaa aagtggaatg
    11041 acgaacccaa gatgaccccc caaacaatcc ccgacaaacc caggcgaggc aaaaagaaca
    11101 caatcccaac gattatcccc aaattgtaaa aaaccggaca gagtgaatac aataaaaact
    11161 ttttataagc ttgagtgata ctgccgtaaa ggttggacag accaagcaaa attggcgata
    11221 aaagcaaaat tctggataac aaaatcattt cctgcctggc ttcttggtca aaccccggcg
    11281 ccagccattc agctaggaaa ggcatcaaca aaaaaacaag acttgagata attgccatca
    11341 caatcaaaaa agaactgaag atgccagcca tgaaatttct ggcttccgca atccgacctt
    11401 cgctgatttt ttggctgagc aggggaatca aaactgttac tgaaacaaaa gaagcgatag
    11461 tggcaaaaat caaatcagga atcctaaacg agctatagta aatatccaac gcggttccgg
    11521 cccccaaact accggccaac aaccggtccc gaatcaaacc caaaaattga gaggtgagag
    11581 caaaaagacc cagtaaaatt gccgcctcat gtaagccggc aacttctcgc ctaaataact
    11641 ttataacctt gttgaccatt ttattctcct agtgatggct cgccttttaa tctttggatc
    11701 gcttgcttta cttcatcgct gtcaggattg gttttttgga tatcaacaaa ttccttgagg
    11761 gcctccgacc tttgccctaa agcttcatag ctcaaagcgc gaaagtattt ggcattggca
    11821 aagtctggtt ccaacgccac cgcttgagaa aaagccctga ttgccccggc gtaatcaagt
    11881 tgtataaatt ttaaataacc aagttgaaaa tagaaagttt ggtctgccgc ttgcggatag
    11941 gcgaccgcga aatcttccag cttctttaag ccactgactg aatcgccgga gtccaagtcc
    12001 agctgggtct ctagtaacac cgccggataa taatcgggct ttaaggcttt ggcttggttg
    12061 agatagtctt tggctttttg agaatcgcct ttttgaattt ccaaccgcgc taaattatcg
    12121 actaaaattg aaggattagt tgggcttaat ttttgggcct ggagataaga atcgagcgcc
    12181 ttttgatatg aaccttccac tcctaaagat accgccaact gataaacgtt tccttgagca
    12241 agccagttta aataattttt tttgtctatc tcaagcgctt tttgcgtatt ggcaaccgcc
    12301 gaattaaaca aagtctcaaa ttgtttttgt ttctgttcca tccccaggtc gttttgcgtg
    12361 agggtattgc ttatcttggc taagtcaatc tccgaagctt tgcgataata taaatcttga
    12421 gcgcttaatt tgatggctct tccaaccaag tcttgagcgc gatctaaatt tttattgtaa
    12481 atcaattcaa cctgtccttt ctgataaaaa tacaaagaca catatttctg aacaaggtaa
    12541 aaaccgctcc ccgataagcc tatgaccaac accaaaatca acaaaactgc ccccaaatta
    12601 agtttagagt cgcgattgag ccaaatctcc aagctcggaa ttattttatt ttcggtcaac
    12661 tgagagataa acaaaccggt cacaaagaaa gtcagtgcca acaaaaaact gtcaggaaca
    12721 taaattaccg aaaaaaccca aagataaatt gagccagcca aggcannnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnggttt tacgtcaaaa attgctactc ctgaaatttt
    12901 ggtccttcca ctcgcccatg attgacctag cccctcaact gaacccagag tcaagaaaaa
    12961 aatcgaaacc ataacaacca aaaaagaaaa accggctaaa gtctttgatt gaagcgagcc
    13021 ctttttaaca agaaaaactt tataaaagaa aagccatagg gaaaaaacta agagcgcgac
    13081 ccaaaccgga agataattga caaaaaccaa ggccactaaa gaaatcccca aacagctagc
    13141 ggcaagtctg cttggcaatg actttctctc agaacgaaac tccaaaaaaa acaggcttga
    13201 gatagccaca aagccgtagt agaccccaaa atcataccac ttcccaacca agctggaaat
    13261 tgaaccgttt gaaaagaagg agaaaaacgg acgcccaaac gcgaaaaaat caagtttaaa
    13321 aacaagaacg acgattgcct gcaaaacaaa aaggaccaag gccgagataa acaaaaccga
    13381 ataaatgctt aaaaatcgac gcttggactg aaaaagactg gctgctaaaa aaagaatagt
    13441 taataaaatg aatagcgtaa aaaacgtgtc ttgttcaaac ccaaaaccaa caattgaccg
    13501 ccaaaaattg cctgaaaaaa cagccgataa gaaacttaga gttaggaccc cgccaagagt
    13561 taataggata cagtttttag gtaaaataat tttattttct tgaagttgcg agattaacca
    13621 aaaaaccagg gcgaccaagg cagccaccgc caaaagaagc tttttcccaa cctcaaaaga
    13681 agccccccaa actgaaaaat tgaaccaggg caaagcaaaa agacaaagag caacaatgac
    13741 taacgacatt ttagcgaaag gccaagggct tattttttcc atttccataa ttcttgttaa
    13801 acggctattg ttttttatta aaacaatttg tgatatattt taagtatata ttattttgcg
    13861 ttttttgcct atggaaaaag gtctcgttac aatcaaatca gccgcggaaa ttcttggagt
    13921 ctctgttgaa actctgcgta attgggaccg gcgcggcgtt ctgaaagcca aacgcagtcc
    13981 gcaaaatggt tatcgcttgt ataatatttc cgaattacaa aaaatgctca agcaaaaaca
    14041 aatcaaagaa ccgcgcgtca aacgcgccaa attggctgat taattccact tgcaaaaact
    14101 acctttttgt tatataataa aagaactgct attaggcaat cacccccaca cccagaatag
    14161 catcgtttgt tatgccccga taatagttct gtggttgttg acataaaaca tgcttgatga
    14221 agttttaaaa gtggttctgg aacaaacagg cataatgcta ttctgggtgt gggggaggaa
    14281 agtccgaaca cagagccgct acgcgcggca agggtagcgg gtaacacccg tctaaagtaa
    14341 tagaggtctc cgacttctat tgcctaagag gtgcgaacag agacgctcaa gccgaaaggc
    14401 aagagcatcc cgtcattggc gggatgagtt cccctagcaa taagggagat gaaacggcta
    14461 aatccttacc tctgtgcaag gccgtgagtg gtagaggttg gaggttagag attggaagtt
    14521 aagaagaatc aagttctcca aataagttct tttttccttt tccattccaa tttccaactt
    14581 ccaacttcta ctactagtgc cgcttgattc gcgccgtaag gcgcagaaca gataaatgat
    14641 tgcccaagtg atagaggttg agggttagag gttagaagtt agaaaaaact aaattcacca
    14701 aacaagagtc ctttcttctc taatctaact tccaattcct aactctcaac ttctattacc
    14761 ggacagaatt cggcttatag atagcagaca tcataaaacc cgccccaaag gcgggtttta
    14821 tgatgtctgc ttttttcttg ttttgttttt ctcttgccgg aaaccggcat ctaatttcgc
    14881 gaccttctgt tctatttctt ttcctccttc gtatccaccg ttgacaatcc gccggctaac
    14941 gggtcaattt cgcaagtgcc gccggcacaa gctaactctt tggaaccggt tgtgacatca
    15001 tcatattcat aaagcacaat tttagaaaag tctatttccg gaaaatcctt ggccatcatt
    15061 tcgtagcgtt ccttagtaat ttcttcataa ggcgctaatt tgtagacata atcattgcgt
    15121 ggcaagaaag atagcccgcc gaccaaatcc cagttttcgt aaagccagtt gccaactctt
    15181 aaccattcat cttctccgac cgacacagtc accgacgggt tatgttcagt gtaattttcc
    15241 tttaacattt tccaatattc caattggtcc atagccgtaa tgtctttttt gaccaccgcc
    15301 ccctccggcg ccttaactgg gaactccaag acgtaggtat tcgcgttggc atcggtttgg
    15361 ccaacttccg ggtggtaagg cacccccata tctttgagca tcaggaagat agggttatga
    15421 cgttcaatcc taactctccg gatatagtat ttggcatgac gcggatggca acccgacgag
    15481 gaatcaaaaa gttgagaacc attgccggac ggcttaacac aggtaactgc cgtagactga
    15541 gcaataccaa aacgtttagc atacttttta tttgtctcta cagccacttt ttgcaacttt
    15601 ttcatcgctt ccggattacg caaggccggg cagtcccact ggccagtaat ggaaacgcct
    15661 agcaatcgct cctcttcgca gtttttgcgc cattccggag acaaatagcc aaacttggtc
    15721 agagttgact ggtaggtgcc aaggatagtg gccactcgca ctttgtcagc caaatccgcc
    15781 aaagtatctt cgcgacgagc tacgacttca gacaggttgc aaaactgctt ggatttcaaa
    15841 acaatttcgc cacagggatt aagaccggaa tattgcgcgt cttttttaaa aatcggccaa
    15901 cgacgcgctg gcagttgttt ttccaggctt cctcggttaa aagtccctcg ttcccccgac
    15961 tgggccttaa tcaagttaag ccattcatcc aaaaattctg cgatcgttgg tttttcgttg
    16021 taaacaacag aattgttggc catcattcgt tccggatggc taatataaaa ttgaccattt
    16081 ttggcttctt tcatttcttg gtcatccaag tctgaaagag aaatcaaggc gctccgccta
    16141 acgcccccca tcaccaccac ctcgccggtc ttacaaataa tatcgtggac atccaaggtg
    16201 gtcagacgcc gaccctgacg agtcaacatt tttgcccgcg aaaaatcaat caggttgcgc
    16261 aaaggctccg ggccggaagc gcgaccgccc atcgtcttta agcgcgcgcc ctgtggccta
    16321 atctctgaat agtcaaaagc aatatccttg ccggcggccc aagtccgcaa accaagaaca
    16381 aaagcgtcag cccacccttc tttgctgtcc ttgataacat gggtcttgag tttctctccc
    16441 gtctgacgct ggataatcgg taattgttca acattctgat gttccaccga ataaccacag
    16501 ccggtgccgc acatcaagac atacataatc tcgccaaaat ctcgccagtc agtcggcgca
    16561 atataagaac aattataagc gcagacatgc gtagcagccg ccgccttgcc cgccgcccac
    16621 aaaagacgca tggagcccat cgccctcata gacaacatat tgtggcgaat ttcgtcatat
    16681 tcttttttgg tcaacttgcc acccaagttg tcgcgcataa aattcacata acggtccaca
    16741 gtttccatcc aagtttctcg tcggcccttt tcaggaatcc aacgcgaata agtggtgtaa
    16801 taaacgaatt cggacaattg gttccgaaaa tattttttgc tctcggtaac caaatttttg
    16861 acctctcgcg gcacttcgcc tttttgtttg cgaatttccg ctcttttttc acgatacaaa
    16921 atataagcct ttgccgttga ggaatacttc tgcaaaatca actctttttc caccaagtcc
    16981 tggatttctt ccactgtcgg caggtaatct ttcccttgtt cttttttggt gttagaaatc
    17041 aatgttgtga ccgccgccgc caccttggtg gcctcttttt cgcccccttc cgcgaccgct
    17101 ttcatggcct tcaaaaccgc gttggttatt ttgctttcgt caaacgggac aaccgtgccg
    17161 tcacgcttct taacttccgt tactttgctg actttcgtcc caatcttctt actcgttttt
    17221 tgagcggttt tgatattttt tgccatattt ttaataaaaa ttgctaaaaa attgataatt
    17281 tgactaagtt ttgctaaaaa ctactcctat tttaatccaa aacttgtccg agctaaaccc
    17341 taacaaactg ttgataagtt aaaaaattaa atttttttca gtcaaaaaaa gaaccacaaa
    17401 acaaaagcga ggactggcta gcattttccg aattttcgtc ctcacccctg tcaccgtcac
    17461 ctattatgtt ctaatttgcc tcaagacttt ccgcaacgcc ggaatcccct gttccaacag
    17521 tttcaatttc ctcttcttct ctaacctcgg cgataacagc agcctcgttt acttctggca
    17581 caatgtcaga gctagtggtg gcagtcattg ggtctgagga ttcaaccgtc accatttctg
    17641 ttgcctcggt agaggtggac atgagcgtgc cgttgttcat ttccaaagga atagcggttg
    17701 gcaaagtggt agttgccgcc gccgttgtct cacccaaaat ctggtcacaa ttgccttttt
    17761 ctgtaacctc ttcgccgtcc ctcaagaaga tacaatattc ttcgcccgtg cccttgtcct
    17821 ttatcatgat acctcgctta atatccatcc ctcccttaaa ctccaccctc tgttcaaagg
    17881 aaacgctggc taagaaatta acat
//
LOCUS       DAOOLO010000010        16941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_3546_length_16941_cov_5.999882, whole genome shotgun sequence.
ACCESSION   DAOOLO010000010 DAOOLO010000000
VERSION     DAOOLO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_16941_cov_5.999882"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLH96_01150"
     CDS             complement(<1..350)
                     /locus_tag="PLH96_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB60410.1"
                     /translation="MTRDYPLEKVRNIGVIAHIDAGKTTCSERILYYTGVSHKIGEVH
                     EGEATMDWMDQERERGITIASAATTAFWTPTYAAADASQKHRLNIIDTPGHIDFTVEV
                     RRSLRVLDGAVVIF"
     gene            complement(438..917)
                     /gene="rpsG"
                     /locus_tag="PLH96_01155"
     CDS             complement(438..917)
                     /gene="rpsG"
                     /locus_tag="PLH96_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPB60411.1"
                     /translation="MRRPVKKRPPVGGDYVYHSEKVEKLINYIMERGKKNAARKIVYG
                     AFDLIKEKTKTEPLEVFETAIQNVAPNMEIRSRRVGGANYQVPHEVRPERKLSLAFRW
                     LIEAAKSKKGKPMAEKLSEEILAAKNNEGDAVRKRENVHKMAEANRAFAHFAIRRSK"
     gene            complement(947..1366)
                     /gene="rpsL"
                     /locus_tag="PLH96_01160"
     CDS             complement(947..1366)
                     /gene="rpsL"
                     /locus_tag="PLH96_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPB60412.1"
                     /translation="MPTINQLIKKSRKKVAKKSQSVALAHGFNMKKNKPIDYSSPFKR
                     GVCLQVKTTTPKKPNSALRKIARVRLTNGMEVAAYIPGEGHNLQEHSVVMLRGGRVKD
                     LPGVRYHIVRGRLDASGVDKRRQGRSLYGAKKPKAAK"
     assembly_gap    1611..1710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1711..1976)
                     /locus_tag="PLH96_01165"
     CDS             complement(<1711..1976)
                     /locus_tag="PLH96_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016477015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB60413.1"
                     /translation="MATLTIFSIIVLVMSVVIHEVSHGYMAYVLGDPTAKLAGRLTLN
                     PIAHIDPFGSVILPLLLSLLPGGIVFGWAKPVPYNPYNLQAGKWG"
     gene            2106..2309
                     /locus_tag="PLH96_01170"
     CDS             2106..2309
                     /locus_tag="PLH96_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPB60414.1"
                     /translation="MKTCPICDKGSKLVGGYSNRVRATKYNPTGKARKKPNLQWAKTA
                     DGKRILICTQCLKAGKNLTVKTK"
     gene            complement(2404..3252)
                     /locus_tag="PLH96_01175"
     CDS             complement(2404..3252)
                     /locus_tag="PLH96_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB60415.1"
                     /translation="MKWRWVVVVVLLVGNLFVWGVVWQSQPTDFLQVAFLDVGQGDAI
                     LITAPNKNQILIDGGPDKKVIRALGRAVPFFDRSIDLVVATHPDLDHIGGLPDVFDRF
                     AVLAYLAPETASESAAYQALQTKIEAERAQKLLAQEGMKVILDQNTWLEIFNAGTKDG
                     ESNAASIVAKLTYGEINFLLTGDAPLALEERLAYQIGSGLEAEVLKVSHHGAKSGSAE
                     IFLQKTAPRYAVVSSGVDNRYGHPHQETLSRLEASGATILQTKDLGTILFKTDGHNLR
                     SEALSN"
     gene            3445..3939
                     /locus_tag="PLH96_01180"
     CDS             3445..3939
                     /locus_tag="PLH96_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60416.1"
                     /translation="MTLTDEILFFLSNHPGNYKRLRQDLSHNRTFTEDERERQRFNRT
                     ILNTLHKLKKRGLVEGNSNSWKISQLGKKFLFHPKKLNLDKEKTSRPTTKKLIVMFDI
                     PETKRVDRNWLRTQLRKIDFMMVQQSVWLGPAPLPQALVSALKDLGLLEYLKFFEVKE
                     KDII"
     gene            complement(4072..5520)
                     /locus_tag="PLH96_01185"
     CDS             complement(4072..5520)
                     /locus_tag="PLH96_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPB60417.1"
                     /translation="MFQARIFAGLLIAGFLGGVIMSALLGFGWWLVVWFLFLASLFGV
                     LAFLKPDLGRFWLLAVFLLGLSMGAGRLEFASVEKSSLPSDEDVVLVGKIVAEPEERE
                     GNTRLIVKETITGEKILVFTERFPAYFYGETVQLAGELRQPENFETESGQIFEYEKYL
                     AARDIGRQFFYPEIKKVSPAPWSVLGGLIRLKKTFQQKIEKVIPEPASALASGILLGG
                     KSGLGEELEEVFRRVGISHVVVLSGFNLSIVASYILRALFFLPHAWAISGSVLGVLFF
                     SLMVGGGASVARASVMAILGLLARGYGRIYEAGVALVFAGVLMTLWNPKILIFDLGFQ
                     LSFLATLGLIYLSPLIESRLRFVPIRLGLREVISTTLGAQAAVYPWLIYKTGNISLIG
                     FLANIVILPAIPLGMLLSFLTGLAGFLGSFPSLPFAYGSYFVLGYIIKSAQFFARFPL
                     ASVTISAPPLWLVLASYGLLVIFVFGGIKKDR"
     gene            complement(5526..5972)
                     /locus_tag="PLH96_01190"
     CDS             complement(5526..5972)
                     /locus_tag="PLH96_01190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPB60418.1"
                     /translation="MEKIILHTDGGSRGNPGPGGAGVAILDERGKVVREARAFLGVLT
                     NNEAEYKAVLLGLETLKKVFGKEKVKNLAIEARLDSELVCRQLNGEYQIKEEKLVPLF
                     IKIWNLRVAEFKNLTFRHIPREENALADKLANRAMDEAGEEQGELF"
     gene            complement(6014..7774)
                     /gene="recJ"
                     /locus_tag="PLH96_01195"
     CDS             complement(6014..7774)
                     /gene="recJ"
                     /locus_tag="PLH96_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPB60419.1"
                     /translation="MATKKWLKRKPLSKKTEIALADFSPLTRQLLFSRGIETADEASR
                     YLNPSYERDLHDPFLMTDIETAARRVIDAIYRKERVIIFGDYDADGIPGALVIESFFK
                     RIGYNDFEAYIPDRHLEAHGLTTEAVKKFAVEGVKLIVTVDCGITNIEAVVEANKLGL
                     DVIITDHHLTQEKLPPALAIVDAKREDDQYPFKMLAGCAVAFKLVQVILALERFNIPE
                     GWEKWLLDLVAISTISDMVPLEGENRVLALFGLKVLRRTKRLGLQTLLGMLKIKPAYV
                     SEDDIGFMVGPHLNSAGRMSSASQAYYLLRTDDDIEAVMIARHLVEKNKERRVLVDVI
                     LKEADFLFKDKKLPAVIVAGNPDWGLGVLGLAASRLLEKYERPIFLWSRNSRGEIKGS
                     CRSDGSVSLVGLMQAAGGGEFFEAFGGHTVAAGFTLADGQEKELSQKLNQAFKKVEKQ
                     KVENKLEIDSEIMLDEITWGTYEDVEQFGPFGVGNPKPVFLLKNVLLESVRTFGNGGI
                     HLELGFKNSRGGIISAIGFFTCPPTLLKEEFDSRNGHNFEDVNLARGERLDLLVNLEK
                     STFKARPELRLRLVDVKRAE"
     gene            7936..8358
                     /locus_tag="PLH96_01200"
     CDS             7936..8358
                     /locus_tag="PLH96_01200"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPB60420.1"
                     /translation="MDCLFCKIVAGEIPAHKVYEDDKFIAFLDIKPINPGHTLIIPKA
                     HYRNLFDMPYELLAELGPLIKKIALAVKDATGADGINIGWNNEPAAGQLIYHSHTHIM
                     PRFEGDGFEHWRGKSQPTTEKFIELTKDIQGMIYDGKK"
     gene            complement(8413..>9339)
                     /locus_tag="PLH96_01205"
     CDS             complement(8413..>9339)
                     /locus_tag="PLH96_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB60421.1"
                     /translation="DEEASLLSVAKYLSHHQKTKLATDFKQARELLEENNLVLLENLR
                     LWPGEKNNDKFFAKELANLGDIYVNDAFSVSHRAHASVVGLPKLLPSYAGLRLVEEIK
                     NLERVIKPKLPLVVILGGAKFKTKIPLIKKLAKADKIFLGGALANSFFKAQGLEVGLS
                     VVDSDLGYIKPFLKNKNLRLPIDVIVANDKGQTVKDSNGIIKGEAIFDIGPKTLAEIA
                     KVTKGAKTVLWNGPLGWFEKDYTKGTLETAKILAKIKAFTVVGGGDTLAAIKKLKLEN
                     KFDFVSTGGGAMLDYIASGGKLVGIKVLNVKS"
     assembly_gap    9340..9439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9440..10041)
                     /locus_tag="PLH96_01210"
     CDS             complement(<9440..10041)
                     /locus_tag="PLH96_01210"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB60422.1"
                     /translation="MTKKIIIGNWKMNPVSRKEAREIVNGIKKTVASIRKTAVVLCPP
                     VIYLENLIGKKSGKILFGAQDVYWEEKGAHTGNISPVMLKEAGADYVIVGHSERRASG
                     EGGETINRKIKAAMKAGLKVILCVGEKDRDSHGHYLKVLETQLKEALIKIPPRLADKL
                     VIAYEPVWAIGKDATGVETPDGFMHNAIFIRKILSKIINK"
     gene            complement(10084..11559)
                     /locus_tag="PLH96_01215"
     CDS             complement(10084..11559)
                     /locus_tag="PLH96_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60423.1"
                     /translation="MKKFKYNQNGALLINVLVYGSIGVLLIGSLIAWGTVNLKLTNQA
                     VVREKALQIAEAGIDYYRWHLAHNKTDFQDGTGAPGPYVHDFADRTGEVVGQFTLDIT
                     PPELGSTLVTINSTGEVNGFPDLTRTIEVKMAVPSFAKYALLANAVMRFGQETETFGP
                     VHSNEGIRFDGLAHNIVTSAKADYNDPDHDDTGDQNEFGVHTHLNPPPPENSLVSSFR
                     PDEAPPSSLMVRNDVFLASREFPVPAVDFNGLMSTLSEIKTAAEQDGHYFAPSGAYGY
                     NLILKTNDTFDLYKVDSLMPSPKNNCRKPNWTDQEGWGTWSIGAQTLINNYPLPANGL
                     IFVEDSIWIEGQIDGARLTIAVARFPENPSTYANINLNRDLRYTNYDGTDVIGLIAQG
                     NINAGMDSNDILRVDAAMIAKNGRAGRYYYNNNCHPYHIRDTLTLFGMVGTNKRYGFA
                     YSDANGYETRNLIYDGNLLYSPPPFFPLVSENYEIISWREK"
     gene            complement(11559..12176)
                     /locus_tag="PLH96_01220"
     CDS             complement(11559..12176)
                     /locus_tag="PLH96_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60424.1"
                     /translation="MIKDNKKTTGFTLIEILIVSGLLVLFLVALATFGRDLFVFNDRY
                     GLSFSADSNAKSALKKLTAELRAAESSATGGFLLESAASTSLIFFSDPDNDETRERLR
                     YFIEDRSLKRGLTEPSGNPPVYDAGDEKIQTLIADLLTASSTFIYFNNDYDGTASSTP
                     LTEPIDVQVVRMARFVFIIQAPSTRAPAPYTVQSEVMIRNVKDNW"
     gene            complement(12173..13948)
                     /locus_tag="PLH96_01225"
     CDS             complement(12173..13948)
                     /locus_tag="PLH96_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPB60425.1"
                     /translation="MLSVYNKKNSHGFTLVEMVVGAAVFLIVMLGLYEGLSAVSKIAK
                     VSRLRTMALMVANERLELARHLPYGDLGIVDGWPTGKLAAFETLERGGIPWGVTTTVR
                     SIDDPYDGTIGGSPDDTSPADYKLVEVLISCNGCGIEPISVATNIAPLALETANGNGA
                     LFIKTIDASGLPVTEAAVTVTNDSHVPAITVSDVTDNTGTLALVDLPPGALSYKVVVS
                     KNGYSHEETYAAGEDGIANPVNPNVTVSEGAVTQISLPIDKLSTVNLKTIDQFCQGVG
                     SFSFNLSGTKLLGQNPDILKYSQGLVTSAAGYLTINNFEWDNYHILPNDETHDVIGSF
                     PLLSIPVDPDTTNDVFLVLAPKTPKGLLVTAKDGASGLPLDGANVSIVGTSTGAVAEK
                     ITNRGHLRESDWSSAGGEVFNIDVNNPAGEIKLTTILNNYVSEGYYVSTVFDVGSATT
                     TYYQLSWQPATQATSTGTNSVRFQLASSEVLEGPWEFVGPDGTVGSYYTASETNINSI
                     HTNERYIRYKLLLTTEDSSVTPRISDVAITYSSSCLPFGQVYFDSLAFDNYIVTVTRD
                     GYQEFTGEILVSDDWQSFEASLNSL"
     gene            complement(13950..15161)
                     /locus_tag="PLH96_01230"
     CDS             complement(13950..15161)
                     /locus_tag="PLH96_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB60426.1"
                     /translation="MKLFFRAKNEKGEEVTGEREAADKFALARDLRAEGLTVIYAEPA
                     DKKKKSNFWQIDLFGRIRMKDKIVFASNLSAMISAGLSLSRSLEVMERQTSNKRFKKI
                     IHDIAERVDRGETLSSSLSNYPKVFPEVFVAMVATAEESGKLPEALKSVSEQLTKSYD
                     LRRKVKGAMIYPSVIVSVMIIIGVLMMIFLVPTLTATFRELNVDLPLSTRVLIGVSDF
                     MAGNVILVILFGIALVGGLISLWRSKQGRAWLDTISLRLPMIGDLTRQVNSATIMRTI
                     SSLISSGVSMIRTIEITERVVQNHHYKAVMKEAAEKVQKGITLSAVFEAHQNLFPVFV
                     EEVSAVGEETGKLPDMLLKGAIFYEEEVDQVTKNLSTIIEPVLMVIIGVAVGIFAISI
                     IGPMYSLSDAI"
     gene            complement(15220..15594)
                     /locus_tag="PLH96_01235"
     CDS             complement(15220..15594)
                     /locus_tag="PLH96_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB60427.1"
                     /translation="MNEEKIEERMDLRKRCFKFSLEIIKITDHLPQKRSAWVITDQLI
                     RSACSIGANLVEAKGSSSRKEFKKFFEISLKSANETTYWLCLLRDSGLSKKEEINSLL
                     REVDEITKMIGSAVIKLKGPRI"
     gene            complement(15652..>16941)
                     /locus_tag="PLH96_01240"
     CDS             complement(15652..>16941)
                     /locus_tag="PLH96_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPB60428.1"
                     /translation="SLKMVTTTEGEEVGEGELKKLAEDLPIVRIVDTLLSHAISQKAS
                     DIHIESFEDELIIRYRLDGVLHDAMILPKETAPGITARLKVLANLRLDEKRLPQDGRF
                     KIEADGQNVSFRVSTLPTYYGEKIVMRLLPDSVHGFTLEGVGFHGDGLEHIQEAIRRT
                     TGMILVTGPTGSGKSTTLYALLDILNTPDVNISTIEDPIEYQISRINQTQVKPDIGLT
                     FASGLRTLVRQDPDIIMVGEIRDRETAGLAVNAALTGHLVLSTLHTNSAAGSVPRLLD
                     MGVEPFLLVSTVNIIIAQRLVRRLNDNKEEYFLTDAELKELGKKTDLDKLLLVLQKEK
                     VTQEKDWQKIPFYRPKVSAGESEGKDEDGYSGRVGISEVLPMTRTLRDLIISGATSDA
                     IVEEAKREGMMTIFEDGLYKAARGVTSIEEVLRVITE"
BASE COUNT         5004 a         4139 c         3126 g         4472 t
ORIGIN      
        1 tcaaaaatca ccaccgcgcc atccaagacg cgcaaacttc gcctcacctc caccgtaaag
       61 tcgatgtgtc cgggtgtgtc aatgatattc aagcgatgtt tttgactcgc atcagcggca
      121 gcgtaagtgg gggtccagaa agcggtcgtc gcggccgaag caatcgtgat gccgcgttca
      181 cgctcttgat ccatccagtc catggtcgcc tcgccttcgt gcacctcacc aattttgtgc
      241 gaaacaccag tgtaataaag aatacgctcg gaacaagtag ttttgcccgc gtcaatatgc
      301 gcgatgacgc caatattgcg aaccttttcc aggggatagt ctcgagtcat agagaatgta
      361 aaattaaaaa attaaaataa taataaaaac aatttaagtt ttggacgcaa aaacgagcaa
      421 acaaaaatcg ctgattctta tttgcttcgc ctaatcgcaa agtgagcaaa agcgcggttg
      481 gcttcagcca tcttgtgcac gttttctcgt ttccgaacgg catcgccttc gttattcttg
      541 gccgccaaaa tctcttcaga caatttttcg gccatcggct tgcctttttt agacttggcc
      601 gcttcaatca accaacgaaa agccaacgac agtttacgtt ctgggcgaac ttcatgcggc
      661 acctgatagt tggcgccacc aacccgacga gaacgaattt ccatattcgg cgcgacgttt
      721 tgaatcgctg tctcaaaaac ctccaacggt tcagtcttgg tcttttcttt aattaaatca
      781 aaggcgccat aaacaatctt gcgagcggcg ttctttttac ctcgctccat aatgtaattg
      841 atgagttttt caaccttctc cgagtggtag acataatcac caccaaccgg cggtcgtttt
      901 tttactggtc tgcgcatttt cgtttaaaga taattaagaa taaagattat ttggcggctt
      961 ttggtttttt ggcaccgtac aaactgcgac cctgacgacg cttgtccaca ccggacgcat
     1021 ccaaacgacc gcggacaatg tgatagcgaa cgcccggcaa gtccttaacg cgaccgccac
     1081 gcaacattac cacagagtgt tcttgcaagt tgtgaccttc gcccgggata taggcggcaa
     1141 cctccatccc attggtcaaa cgtacgcgag caatttttcg caaagcggag ttaggttttt
     1201 tcggagtagt cgttttcact tgcagacaaa cgccacgctt gaacggagac gaataatcaa
     1261 tcggcttgtt ttttttcata ttaaaaccgt gcgccaaagc cactgattgg gattttttgg
     1321 ccaccttctt gcgtgatttt ttgattagtt gattgattgt tggcacgata aataatttaa
     1381 actgtaaaat ttagactgca actcgttatt ttagtccaaa agacgaccaa atcaaagagc
     1441 gccaaccctt tgcctggtct cttttactgt aatacaaaag ccaaaaaaat gcaatatgtt
     1501 ttgaggcttg gctatatctt tagaagaaga ggttaaaaag ccatcccagt caaagcggta
     1561 aaaattaacg gcaataactt gtcaatcacg aagccggcca ctagaacagc nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccccacttgc cagcctgaag gttataaggg
     1741 ttataaggca ccggcttggc ccaaccaaag acaatcccac ccggcaagag tgagagcaaa
     1801 agaggtaaaa tgacagaacc aaacgggtca atgtgcgcaa tagggtttag ggtcagacga
     1861 ccggccaact tagcggttgg gtcgcccaaa acataggcca tataaccgtg cgacacttcg
     1921 tgaatgacca cggacatgac caaaacgata atcgaaaaga tggtgagggt tgccataatt
     1981 gagaaaaggg gtctagcgtc cgcgcggcgc ggccgctaga cttttattta attttcttgc
     2041 caaaaagtta ttttatgata acatttaaca gtccgtttgt aaaaagcggc cattacctaa
     2101 aaatcatgaa aacctgcccc atttgtgaca aaggctcaaa actggttggt ggttactcca
     2161 accgcgtgcg cgccactaaa tataacccga ccggtaaagc tcgcaagaag cctaatttgc
     2221 aatgggccaa aaccgctgat ggcaaacgaa tcctaatctg tacccaatgc ctgaaagccg
     2281 gcaaaaacct aactgtcaaa accaaataaa gaaaaaaccg gacaaacctg agtaaactgg
     2341 agagggtaac cctctccagt tttattttaa cggcgtttag gctccaaaat tgagggtggt
     2401 cgctcaatta gacaaagctt cagaccgcag attgtggcca tctgttttaa agagaattgt
     2461 ccctaaatct tttgtctgca aaatcgtcgc tccgctcgcc tccagtcgcg acaaagtttc
     2521 ttggtgagga tggccataac ggttgtcaac gccgctggaa acaacggcgt agcgcggggc
     2581 ggttttttga agaaaaatct ccgcgcttcc gctcttggcg ccgtggtgac taactttcag
     2641 gacttccgct tccaacccgg aaccaatttg ataagccaat ctttcttcta aggccaacgg
     2701 cgcgtctcct gtcaataaaa aattaatttc cccataggta agcttagcca caatcgaagc
     2761 cgcgttactt tcgccgtctt tggttccggc gttgaaaatt tccagccatg tattttggtc
     2821 caaaattact ttcatcccct cttgagccag aagtttttgc gccctttctg cctcaatttt
     2881 tgtttgcaaa gcttggtaag cggcgctctc gcttgcggtt tctggcgcca agtaagccaa
     2941 gaccgcgaaa cggtcaaaga catccggcaa cccgccaatg tggtccaaat ccggatgagt
     3001 agcaaccacc aaatcaattg accgatcaaa aaaaggcacc gcgcgcccca gggcgcgaat
     3061 aacttttttg tctggcccgc cgtcaatcaa aatttgattt ttatttggcg ccgtaatcaa
     3121 aatcgcgtcc ccctggccaa catccaaaaa agccacttgc aaaaaatctg tcggttgaga
     3181 ttgccaaaca accccccaaa caaaaagatt accaaccaac aaaacaacga ccacaaccca
     3241 ccgccatttc atatttttga aatattaaaa aaacttaaga ctagcctctg ctattttata
     3301 acaaacaata tttaaatgtt accaaatttt attctaacca atccttcctt tatccacagt
     3361 ctaccatttt atgcggccgc ataaaatggt agacttgcta atgatcaatt atttattact
     3421 tattcactta actactaacc taaaatgacc ttaaccgatg aaattttatt ttttctatca
     3481 aaccacccgg gcaactacaa aagactaaga caggacctaa gtcataatcg aacttttacc
     3541 gaagacgagc gagaaaggca aaggtttaac agaacaatac tcaacacttt gcataaactt
     3601 aaaaaaagag gcttggtgga aggaaacagc aattcttgga aaatttccca actaggaaaa
     3661 aaattccttt ttcatccgaa aaaactgaat ttggacaaag aaaaaacaag caggcccacc
     3721 acaaaaaaac tgattgtaat gtttgatatt ccagaaacaa agagagtaga tagaaactgg
     3781 ctaagaactc aattaagaaa aatagatttt atgatggttc aacaaagcgt ttggctgggg
     3841 ccggccccct tgcctcaagc tctcgtttca gccctaaaag acctggggct tttagagtac
     3901 ctaaaatttt ttgaggttaa agaaaaagat attatctaaa actttttaca ctctttttcg
     3961 cgccctaaag taatcagaga ttatctccta tattatgcgg ccgcataata taggagataa
     4021 ttcttttttg tcaaaagcaa gtaaattttt gttaaaaaca aataaaaatt cttatctatc
     4081 tttttttatt cccccaaaaa caaatattac caagagccca taggaggcca gcaccaacca
     4141 gagaggcggg gcggagattg ttactgaagc caacggaaaa cgcgcgaaaa actgcgctga
     4201 tttgataata tatcccagaa caaaataaga accatacgca aaaggcaggg acggaaagct
     4261 ccccaaaaat ccggcgagac cggtcaaaaa agacagaagc atccccaaag gaatcgccgg
     4321 caggatgaca atgttagcca aaaaaccaat taaagaaatg ttgcctgttt tgtatatcaa
     4381 ccagggatag acggctgctt gggcgcccag ggttgttgag ataacttcgc gcaagccgag
     4441 cctgatcggc acaaatctca ggcggctttc aattaaaggt gacaaataaa tcaacccaag
     4501 cgtcgccaaa aacgacaact ggaaccccaa atcaaagatc aagattttgg ggttccataa
     4561 agtcatcaaa acaccggcaa aaaccagagc cacgccagcc tcataaattc gcccataacc
     4621 acgagccaac agacccaaaa tcgccattac cgaggcgcga gccactgatg ctcccccacc
     4681 aaccatcaaa ctaaaaaaca aaacccccaa cacgcttcct gatattgccc aagcgtgagg
     4741 caagaaaaac aaggctctca gaatatagct cgcgacgata gacaaattga agccggataa
     4801 gaccaccaca tgagaaatac cgacccgacg aaagacttct tctaattctt cgcctaaacc
     4861 ggacttgcca ccaagcagta ttccggaagc cagagctgac gccggctcgg gaataacttt
     4921 ttctattttt tgctgaaaag tttttttcag tctaatcaac cctcctagca ccgaccacgg
     4981 ggccggtgaa acttttttga tttcgggata aaaaaactga cgaccaatat cacgcgcggc
     5041 caaatatttt tcatattcaa aaatttgccc actttcagtc tcaaaatttt ccggttggcg
     5101 caattcgcct gctagctgaa cagtttcacc gtaaaaatag gccgggaatc tctcggtaaa
     5161 aaccaggatt ttttcgccgg ttattgtttc tttaactatc aaccgagtat tgccttctcg
     5221 ttcctccggt tcagcgacta tcttgccgac caaaacaaca tcttcatccg acggcaaaga
     5281 tgatttttca accgaggcga actccaaacg tccggccccc atcgacaaac ctaacaaaaa
     5341 aacagctaac aaccaaaagc gtccaagatc cggttttaaa aaagcgagaa cgccaaataa
     5401 actggccaaa aacaaaaacc aaacgaccaa ccaccaacca aagcccagta acgccgacat
     5461 tatcacgcct cccaaaaaac cggcaatcaa gagcccggca aaaattctag cttgaaacat
     5521 attttttaaa acagctcgcc ctgctcttca ccggcttcat ccatcgcccg gttagccaac
     5581 ttgtcagcca aggcattctc ttcgcgcgga atatgtctaa aggttaaatt tttaaattcc
     5641 gcgacccgca agttccaaat ttttataaaa agcggcacaa gtttttcttc tttgatttgg
     5701 tactcgccgt ttaattgacg acaaaccaat tcgctgtcca accgggcttc tatcgccaaa
     5761 tttttcactt tttctttacc gaatactttc ttgagggtct ccaaacccaa caaaaccgct
     5821 ttgtactccg cctcattgtt ggttagcacc cccaaaaaag ccctagcctc cctgaccacc
     5881 ttccccctct cgtcaagaat ggccacccca gcgccacccg gaccgggatt cccgcgcgag
     5941 ccgccatcgg tgtgtaaaat tattttttcc atttttttat tatagcctgc tcaccaagaa
     6001 aaaaccacgt tatttactcg gcccgtttaa cgtccaccaa gcgcaagcgc aattccggcc
     6061 tggctttaaa agtcgatttt tctaaattga ccaataaatc caatctttcg cctcgcgcta
     6121 aattaacatc ttcaaaatta tggccatttc gagaatcaaa ctcttcctta agcaaggttg
     6181 ggggacaagt gaaaaaacca attgccgaaa ttatcccacc tcggctgttt ttgaaaccaa
     6241 gttccaagtg aatgccaccg ttaccaaaag tgcggaccga ctcaagcaaa acattcttga
     6301 gcaaaaaaac cggttttgga ttgccaacgc caaagggacc aaattgttca acgtcttcat
     6361 aggtgcccca cgttatttca tcaagcataa tttctgaatc tatctccaac ttattttcca
     6421 ctttttgctt ttcaactttt ttaaaagcct gatttaactt ttgagacaat tctttttctt
     6481 ggccatcagc cagagtgaaa ccggcagcca ccgtatgccc cccaaaggcc tcaaaaaatt
     6541 cgccgccccc ggcagcttgc atcaacccca ctaaactgac cgaaccgtca gaccggcacg
     6601 aacctttaat ttccccacgg ctgtttcgcg accacaagaa aatcggtcgt tcatattttt
     6661 ccagcaaacg actagccgcc aaacccaaaa cgcccaagcc ccaatcgggg ttgccagcga
     6721 cgataaccgc tggtaatttt ttatctttga ataagaagtc ggcttctttc aaaataacat
     6781 ccaccaaaac acgacgttcc ttgttttttt ccaccaaatg acgagcaatc atcaccgcct
     6841 caatgtcatc atcagtgcgc aaaagataat aagcctgcga agctgacgac attcggccgg
     6901 ccgaattgag atgcggaccc accataaagc cgatatcatc ttccgaaacg taggccggtt
     6961 taattttgag catcccgagc aaagtctgaa gacctaggcg tttggttcgg cgcaaaacct
     7021 tcaggccaaa gagcgccaag acacgattct ctccctcaag cggcaccata tcgctaatgg
     7081 ttgaaatagc caccaggtcg agcagccatt tttcccaacc ttcaggaata ttaaaacgct
     7141 caagcgccaa aattacttga accaacttaa aggccacggc gcaaccggca agcattttga
     7201 aaggatattg gtcatcttcc cgtttagcgt cgacaatcgc gagcgccggc ggcaattttt
     7261 cttgtgtcag atggtggtcg gtgataatca catccaaacc caatttgttc gcttcgacca
     7321 ccgcctcaat attcgtaatg ccacaatcaa ctgtgacaat caacttgact ccttccacgg
     7381 caaatttttt aaccgcttct gttgtcaggc catgtgcttc taaatgacga tctggtatat
     7441 atgcctcaaa atcgttgtaa ccaatccgtt taaaaaaaga ttcaatcacc agagcgccgg
     7501 ggatgccatc cgcgtcataa tcaccaaaaa taattactct ttctttgcgg taaatcgcat
     7561 caataacccg acgagcggcc gtttcaatat cggtcatcaa aaaggggtcg tgcaaatctc
     7621 gctcgtaaga cgggttcaga taccttgacg cttcatcggc cgtctctatc ccccgactga
     7681 aaagcagttg acgcgtgagc ggtgaaaaat ccgccaaggc gatttctgtt ttcttactta
     7741 gcggcttgcg tttaagccat tttttggttg ccatagatat attataacac aacttggttt
     7801 gccctaaatt tctggcgttt ttcaattttt ctctttaaac catctgcaaa atacgctata
     7861 ctatctaaaa atcaggggtt tggttatcgt tgttctttat ttttgatttt aatttttaca
     7921 ttttaaatta tttatatgga ttgtcttttt tgcaaaattg tcgctggcga aatcccggct
     7981 cataaagttt acgaggatga taagtttatc gcttttttgg atattaaacc gataaacccc
     8041 ggccacactt tgattattcc caaagctcat tatcgtaacc tttttgatat gccatatgaa
     8101 ctcttagccg aactgggccc cttaatcaaa aagattgcct tggcggtcaa agacgcgact
     8161 ggcgccgatg gcatcaatat tggctggaac aacgaaccgg ccgccggcca gcttatttat
     8221 cactcacaca cgcacattat gccgcgtttt gaaggcgacg gttttgaaca ttggcgcggc
     8281 aaatctcaac caacgacgga aaaatttatt gaattaacca aagatattca agggatgatt
     8341 tacgacggaa aaaaataacg caatcttaat ttaacttaaa tgcttttaac cttaactttt
     8401 gacttctaaa atttaacttt taacattcag tactttaatc cccaccaatt ttccgcccga
     8461 agcgatatag tcaagcattg cgccaccgcc agtagaaaca aagtcaaact tgttttccaa
     8521 cttaagtttt tttatggccg ccaaagtatc accgccgccc accactgtaa aagctttgat
     8581 cttggccaga atcttggccg tttccaaagt gcccttggtg taatcttttt caaaccaacc
     8641 aagtggccca ttccacagaa ccgtcttagc gcccttggtc accttggcaa tctccgccaa
     8701 agtcttagga ccaatgtcaa aaatcgcttc cccctttatt attccatttg aatctttaac
     8761 cgtctgcccc ttgtcattcg ccacgattac atcaatcggc aatctcaaat ttttattttt
     8821 taaaaacggt ttaatataac ccaagtcact atccacgacg gataatccta cttctaaacc
     8881 ttgagctttg aaaaaagagt tagccaaagc tccgcctaag aatattttat cggccttggc
     8941 caatttctta atgagaggga ttttggtctt aaacttggca ccacccaaaa taaccactag
     9001 tggcaacttg ggttttatta ctcgctccag attttttatc tcttctacca aacgcagacc
     9061 agcataactc ggcagaagtt tgggcaagcc aaccaccgaa gcgtgagccc gatgcgaaac
     9121 cgaaaaagca tcgttgacat aaatatcacc caaattagcc aactctttag caaaaaactt
     9181 gtcattgttt ttttctccag gccataaacg caaattttcc aataaaacca agttattttc
     9241 ctccaacaat tccctcgctt gcttaaagtc agttgccaat tttgtttttt ggtggtgact
     9301 caaatattta gcaaccgaca gaagtgaggc ctcttcatcn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnt ttttattgat tattttactc aaaatttttc gaataaaaat
     9481 cgcgttatgc ataaaaccgt ccggcgtttc aacaccggtt gcatctttgc caatcgccca
     9541 gactggttca taagcgatga ccaatttgtc ggccaaacgt ggcggaattt taatcaaagc
     9601 ctcttttaat tgcgtttcca aaactttcaa ataatgacca tgggaatcac gatctttttc
     9661 gccgacacac agaatcactt ttaagcccgc tttcatcgcg gcttttatct tgcgatttat
     9721 ggtttcacca ccttcaccgc tagcccgcct ttccgaatga cctacaataa cataatcagc
     9781 ccccgcttcc ttaagcatta ctggcgaaat gtttccggtg tgagcgcctt tttcttccca
     9841 gtaaacatcc tgcgcaccaa ataggatctt accagacttc ttgccaatca aattttccaa
     9901 gtaaataacc ggcggacaaa gaacgacggc tgttttgcga atacttgcca ccgtcttttt
     9961 tatgccattt acgatttctc gcgcctcctt gcggctcacc gggttcattt tccagttgcc
    10021 gataattatt tttttagtca taaattaatt ctaccctaaa ccgtctttaa cacaaaatca
    10081 aaattacttc tcgcgccaag agattatttc atagttttcg gataccaagg gaaaaaatgg
    10141 aggggggcta tagagaagat taccgtcata aatcaagttc ctggtttcat aaccatttgc
    10201 atcagaataa gcaaaaccat agcgtttatt ggtgccaacc atgccaaaca aagtgagggt
    10261 atcgcgaata tgataaggat ggcagttgtt gttgtaataa tagcgaccag cccgaccatt
    10321 tttagcaatc atggctgcgt caacacgcaa gatatcatta ctatccatgc cggcgttaat
    10381 attgccctga gcaatcaagc caattacgtc tgtgccatca tagttggtat aacgaagatc
    10441 gcgattaagg tttatattgg cataggtgct gggattttcc ggaaaacgtg ccacggcaat
    10501 agttaaacga gcgccatcaa tttgaccctc tatccaaata ctatcttcca caaaaattaa
    10561 accattagca ggtaaggggt aattgttaat taaggtctgg gcccctatac tccaagttcc
    10621 ccaaccttct tgatcggtcc aattcggctt gcgacagtta tttttaggtg aaggcattag
    10681 tgaatcaact ttgtatagat caaaagtgtc atttgttttc aaaatcaaat tataaccata
    10741 agcgccagaa ggtgcaaaat aatgaccgtc ttgttccgcc gccgttttta tctcgcttaa
    10801 agtcgacatt agaccattga aatcaaccgc cggcaccgga aactcgcgac tagccaaaaa
    10861 gacatcattg cgtaccatta aggaacttgg cggcgcctca tccggacgga aagaagaaac
    10921 taaactattt tccggtggtg gtggattcag gtgagtgtgg acaccaaact cattctgatc
    10981 acctgtgtca tcgtgatctg ggtcattata atcagccttg gcactggtta caatgttatg
    11041 agccaaaccg tcaaagcgaa taccttcgtt agagtgaacc ggtccaaaag tttctgtttc
    11101 ttgaccaaaa cgcattaccg cattggctaa taaagcatat ttagcaaaag acggtaccgc
    11161 catttttact tctatggtgc gagttagatc ggggaatcca tttacctcac cagtagaatt
    11221 aatagtcacc aaagtggagc ccagttccgg tggtgtaata tccagagtaa attgaccgac
    11281 aacctcacca gttcgatcgg caaaatcatg aacataaggc ccgggtgcgc cggttccatc
    11341 ctgaaaatca gttttattat gtgccaaatg ccagcgataa taatcaatac ccgcttcagc
    11401 aatttgtaac gctttttctc gaacaaccgc ttggttggtc aatttcaagt taaccgtccc
    11461 ccaagctatc aggctaccga tcagtaaaac tccaattgaa ccataaacga gaacattgat
    11521 tagcaaagcg ccgttttgat tatatttaaa ctttttcatc accagttatc ttttacgttt
    11581 ctaatcatta cttcactttg aaccgtgtag ggtgcggggg cacgggtgct tggcgcttga
    11641 attataaaaa caaaacgtgc cattctgaca acctggacgt ctatcggttc agttaagggt
    11701 gtagaagaag cggtaccatc atagtcatta ttaaaataaa taaaagtact agaagcggtg
    11761 agaagatcgg caatcagagt ctgaattttt tcatcaccgg catcataaac aggcgggttg
    11821 cccgaaggtt cggttaagcc acgtttgaga gatcgatctt caataaaata gcgcaggcgt
    11881 tctcttgtct cgtcattatc gggatcagag aaaaaaatca acgaagtgga ggcggccgat
    11941 tcaagcaaaa aaccgccagt cgctgaagat tctgcggctc gtaattcagc cgtcaacttc
    12001 tttaaagccg acttggcgtt gctatcagcc gaaaaagata aaccataacg atcattaaaa
    12061 acaaacagat cgcggccgaa tgttgccaaa gcaaccaaga acaaaaccaa aagtccggac
    12121 acaatcaaaa tttctattag ggtaaaacct gtggtttttt tattatcttt tatcataacg
    12181 aattcaaaga agcctcaaag ctttgccaat catcagaaac caaaatctcg cccgtaaact
    12241 cctgataacc gtcacgagtt actgtaacaa tataattatc aaacgccagt gaatcaaaat
    12301 aaacttgacc aaaaggcaaa cacgaggaac tataagtaat cgccacatcg gaaatccgcg
    12361 gtgttacaga agaatcctct gtcgtaagca ataatttata acgaatataa cgttcgttcg
    12421 tatgaatact gtttatgttt gtctcagatg cagtgtaata agaaccaact gtgccatcag
    12481 gaccgacaaa ttcccaaggg ccttccaata cctcacttga agccaactga aatctaacgc
    12541 tgttggttcc ggtagaagta gcttgggttg ctggttgcca acttaactga taataagtag
    12601 tagtcgccga accgacatca aaaacagttg atacataata accctctgat acataattgt
    12661 ttaaaatagt ggtcaatttt atttcaccgg ctggattatt aacatcgata ttaaaaacct
    12721 ccccaccagc cgaagaccaa tcagactccc taagatgacc acggttagtt attttttcag
    12781 ctacggcacc agtggaagta ccgacaattg aaacattggc accatcaagc ggtaagccac
    12841 tggcgccatc tttcgctgtc accaaaagac cttttggcgt cttgggagct aataccaaaa
    12901 aaacatcatt ggtcgtatcg ggatcaactg gaatagaaag caatggaaaa gagccaatca
    12961 catcatgggt ttcatcatta ggcagaatat gataattgtc ccactcaaaa ttattaattg
    13021 taagatagcc ggccgcgctg gtaaccaggc cctggctgta cttcaagata tccggatttt
    13081 gccccagcaa tttggtgccc gataaattaa aagaaaagga gccaactccc tgacaaaatt
    13141 gatcaatagt tttcaaatta acagtactca acttatcaat tggcaaactg atttgagtga
    13201 cggccccttc agacacggtc acattcggat taaccggatt ggcaataccg tcttcaccgg
    13261 cggcataagt ttcctcatga ctgtaaccat ttttactaac cacaacctta taggagagtg
    13321 ctccgggcgg caaatcaacc aaagcaagtg tccctgtatt atcagtgaca tcactaacgg
    13381 ttatggccgg gacatgagaa tcattagtca ccgtcacagc cgcttcggtc accggcaacc
    13441 cgctagcatc aatggttttt ataaacaaag ccccgttgcc attggctgtt tccagagcca
    13501 ggggtgcaat attggttgcc accgaaatag gctctatccc acaaccatta cagctaatca
    13561 aaacttctac caacttataa tcagccggcg aagtatcatc aggactcccc ccaatcgtgc
    13621 catcataagg gtcatcaata gaacgaacag ttgtggttac cccccacggg ataccgcctc
    13681 gttcaagagt ttcaaaggct gctaacttgc cagttggcca accatcaaca atacccaagt
    13741 caccataagg caagtgtcta gccaactcca gtctttcatt ggccaccatg agtgccatcg
    13801 tgcgcaatct agagaccttg gcaatcttgc tcaccgctga taacccttca taaagtccca
    13861 gcattacaat caagaataca gcagcgccaa ccaccatttc caccaaggtg aaaccatgac
    13921 tgtttttttt gttataaact gacaacattt taaatagcgt ctgataacga atacatcgga
    13981 ccaataatag agatggcaaa aataccgacg gccacaccaa ttattaccat caaaaccggt
    14041 tcaatgattg ttgaaagatt tttagtcacc tgatctactt cttcctcata aaaaatcgcc
    14101 cccttcaaaa gcatgtccgg cagttttccg gtttcttcgc caaccgccga tacttcttcc
    14161 acaaacaccg ggaataaatt ctgatgggct tcaaaaacgg ccgacaaggt aatgcctttt
    14221 tgtacttttt cggccgcctc tttcataact gctttgtaat gatgattttg cacgactcgt
    14281 tcagtaattt ctatcgttct aatcatacta acccctgaac taattaaaga agaaatcgta
    14341 cgcataatcg ttgccgagtt aacctggcgc gtcaaatcac caatcatcgg caaacgtaga
    14401 gaaatcgtat ctaaccaagc ccggccttgt tttgatcgcc ataaagaaat taatccgcca
    14461 actaaagcaa tcccaaaaag aatcaccaaa atgacatttc cggccataaa atcactgacc
    14521 ccaatcaaga cccgggtcga caaaggcaag tccacgttca actcacggaa agtggccgtc
    14581 agggttggaa ccaaaaatat catcatcaag acaccaatta ttatcatgac cgagacgata
    14641 accgacggat aaatcatggc ccctttaact ttgcgtcgca aatcataact cttggtcaat
    14701 tgttcactga cactctttaa ggcttccggc aacttacctg attcttcggc ggttgcgacc
    14761 atcgccacaa aaacttccgg aaaaactttg ggataattgg acaaagagct acttagggtt
    14821 tcgccccgat ctactcgttc agcaatatca tgaataattt ttttaaatcg cttgtttgaa
    14881 gtttgtcttt ccatcacctc aagtgaacga gatagggata gaccggcgct gatcatagcg
    14941 cttagattgc tcgcaaaaac aatcttatct ttcattctaa ttcgaccaaa aagatctatt
    15001 tgccaaaaat ttgacttttt tttcttatcg gccggttcgg cataaatcac agtcaggcct
    15061 tccgcacgca aatctctggc caaagcaaat ttatccgccg cttcacgctc accagttact
    15121 tcttcgcctt tttcattttt tgctcgaaaa aataatttca tataataatt tagaagttaa
    15181 aagtcagaag tcaaaagttt aaataattat gtaaaaagtt cagattcttg gtccttttaa
    15241 ttttatcaca gcactaccta tcattttagt aatttcatct acttctctta acaaagagtt
    15301 aatttcttcc tttttggata agccactatc tctaagcaga caaagccaat atgttgtttc
    15361 atttgcgctt tttagtgaaa tttcaaaaaa ctttttaaat tcttttctag agctagaacc
    15421 tttagcttca accaagtttg ctccaataga acaagctgat cttattagtt ggtctgtgat
    15481 cacccaggca cttctttttt gtggtaaatg atctgtaatt tttattattt ctaaactaaa
    15541 tttaaaacaa cgtttcctga ggtccattct ttcttcaatc ttctcttcat tcataactta
    15601 tctacttttg attttaactt ctagcttttg acctttaact tttaacatga cttattctgt
    15661 tatcaccctc aacacttcct caattgacgt aaccccacgc gccgccttgt aaagaccatc
    15721 ttcaaatatc gtcatcattc cttctcgctt ggcctcctca acaattgcgt cagaagtcgc
    15781 gccggaaatt atcaaatccc gcaaggtccg agtcataggc aaaacctcgc taataccaac
    15841 tcgtccggag taaccgtctt cgtctttccc ctcgctctcg cctgctgaaa ctttggggcg
    15901 gtaaaacggt attttttgcc agtctttctc ttgagtcact ttttcttttt gcaaaaccaa
    15961 gagcaattta tctaaatcag tttttttacc aagctcttta agctccgcgt cagtcaagaa
    16021 gtattcttct ttgttgtcat ttaaacgcct aaccaaacgc tgggcaataa taatattaac
    16081 cgtagaaacc aaaagaaaag gttccactcc catatcaagc aaacgcggaa ctgaaccggc
    16141 ggccgaattg gtgtgcaagg tggatagcac caagtggccg gtcaacgccg cgttaaccgc
    16201 caaaccagct gtttcgcggt cgcgaatttc tcccaccatg ataatatccg ggtcttgacg
    16261 aactagcgtc cgtaaaccgg acgcgaaagt cagaccgata tccggcttaa cttgagtttg
    16321 gtttatacgc gaaatttgat attcaatcgg gtcttcaatt gtggaaatat taacatccgg
    16381 cgtgtttaaa atatcaagca aagcatagag ggtggttgac ttgccagaac cagttgggcc
    16441 ggttaccaaa atcatgccag ttgtgcgacg aatcgcttcc tggatgtgct ccagaccatc
    16501 tccatggaaa ccaactccct ccagggtaaa accgtggacc gaatcgggca aaagacgcat
    16561 tactattttt tcaccataat aggtcggcaa cgtagaaacg cggaaagaaa cattttggcc
    16621 atcagcttca atcttgaaac gaccatcctg cggcaaacgc ttttcatcta gccgcaaatt
    16681 tgctaaaact tttaggcgag ctgtgatacc cggggccgtc tccttgggca aaatcatcgc
    16741 gtcatgcaaa acaccatcta aacgataccg aattattaat tcatcttcaa aagattcaat
    16801 gtgaatatcg gaggcttttt gggaaattgc gtgagatagt agagtatcaa cgatacgaac
    16861 aatcggtagg tcttccgcca actttttgag ctctccctcg cctacttctt caccttcagt
    16921 tgtcgtgacc atcttgagcg a
//
LOCUS       DAOOLO010000011        16202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_3798_length_16202_cov_6.481142, whole genome shotgun sequence.
ACCESSION   DAOOLO010000011 DAOOLO010000000
VERSION     DAOOLO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16202
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3798_length_16202_cov_6.481142"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLH96_01245"
     CDS             <1..890
                     /locus_tag="PLH96_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60429.1"
                     /translation="KEKEVGTPRMGGIVIWGSVIATTIIFWLLQTVFPSEASSKLDFV
                     SRNQTWLPLFTLLVGSIVGLVDDILQVRPTENSAKAGLSFYKRLAIVLALGLIGGYWF
                     YFKLGMDSVSLPFIGEWQIGFWLVPLFVLVMIALFSSGVIDGLDGLSGGIMAIIFAAY
                     TGIAYFQSQIDLAAFCAVVTGGILAFLWFNIPPARFYMSETGILGLTTTLTVVAFLTD
                     SVFTLPIIALPLLITSLSVIIQLLSKKVRGRKVFLVAPLHHHFEAIGWPAYKVVMRYW
                     VIGIICGIIGMILTLISKF"
     gene            892..1995
                     /gene="ftsW"
                     /locus_tag="PLH96_01250"
     CDS             892..1995
                     /gene="ftsW"
                     /locus_tag="PLH96_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPB60430.1"
                     /translation="MKTAKTAKPDRFLFSLILILITGGFVIFSSAALGQVGRTSASFS
                     VVALKQLLVLFLGFVMMLIASKIPYKRWRKYSPYIFALALVLTSLVFVPGIGFGTNGA
                     RRWLDLGFTTFQPSEFLKFGLIVFLAAWLAARKEKIRNLKEGFLPFLIFLSIVCLLLL
                     KQPDTGTLIVAIFAALSLFMIAGGRWSHLLIIILLGILLAGALIWTRPYVKDRLVTYL
                     DPQKDSRGASWQINQSLIAIGSGGLVGRGFGQSIQKFNFLPEPIGDSIFAVAAEEFGF
                     IGSTIIVGLFLLLALWGLKLMNKVKDSFGRLLGIGLVLLITVQSFVNIGAMIGIFPLT
                     GLPLLFISQGGSALFFALIEVGIILNISKEVRK"
     gene            2006..3133
                     /gene="murG"
                     /locus_tag="PLH96_01255"
     CDS             2006..3133
                     /gene="murG"
                     /locus_tag="PLH96_01255"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPB60431.1"
                     /translation="MKILFTGGGTGGHFYPVIAVAEEVKRICTEQKIVAPELYFMSDK
                     PYNERALLDNGLKFVPVQAGKIRRYFSLMNFFDVFKTIFGSIRAIFTLFSIYPDVVFG
                     KGGYASFPGLLAARLLRIPVVIHESDSHPGRVTLWAGKFAKRIAVSYPEAAKYFPPEK
                     TAVVGNPIRREVLFPAKAGAHEFLGLDKNIPIIFVVGGSQGAVRINDVVLDILPQLLA
                     DYQVIHQVGKSNYDDLKNRASFLTENNPHKNRYKLFDYLNDVAMRMTAGAANLVISRA
                     GSAIFEIANWGIPAIIIPIPEEISHDQRTNAFTYARSGGAVVIEEKNLTPSILLSETK
                     RILDDPELQKQMKTGAQSFAKPEAGEKIAEEIIRLALEHEE"
     gene            3123..4454
                     /locus_tag="PLH96_01260"
     CDS             3123..4454
                     /locus_tag="PLH96_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPB60432.1"
                     /translation="MKNRVITRVAPSPTGLMHIGTARVALFNHLFTKQNDGKIILRIE
                     DTDRERSKPEFEKNIKDGFSWLGLTFDEIWKQSERTEIYTQYLQKLLAEDLAYVSQEE
                     AKKEGDRSSVIRFRNPGQKITFQDLIHGEITFDTTELGDFVIAKDLETPIFHFANVVD
                     DLEAGITHIIRGDDHISNTPRQILIFKALGGQIPIYTHLPMILAPDKSKLSKRHGAWP
                     VTEYRDEGYLKEAVLNFIVLLGWSPQSRAEIAQDGTEDIVSLDQMLKLFDLAKVQKKG
                     AVFNLEKLNWLNREHLKRLPTTEQWQKIIDWLPPTKADFMKTNELMLQKITPLILERV
                     STLKEAQALLANGEFDYFFHQPKPSQELLRTTTHLTEIASRLSELAAEDFTAETIKNK
                     IWDFATEKGRGEVLWPLRVALSGKEKSPDPFVLAAILGRAETLNRIKNALS"
     gene            4441..5745
                     /locus_tag="PLH96_01265"
     CDS             4441..5745
                     /locus_tag="PLH96_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPB60433.1"
                     /translation="MRFLSYNSQISIIVVIILLFSGFAWRDNKSAVQAATADELRNKI
                     ENSQSEIQKLEKEIQTFRQEITKTQQEKNSLNTEIKKIDLTRQKLGSEIKLTQNQIKT
                     TEKQIAELANGIQDKETAIEHNRAVIAESMRLLAGTDDESLLVSFLASESLSEFWNSA
                     DQLAQVNNRLIKLIAELNQNKKQLTSTKVQKEVQKKDLSVLTTKLSDQKQLVDQTKQE
                     KNSLLSATQSKETAYQKLLQDRLAKKRAVEAEMQQAEAQLKVVLDPNRLPDSGTKALR
                     WPLDKIIITQYFGNTAFALQNAAVYNGKGHNGIDLAADVGTPLKAANSGKVIGVGDTD
                     NTCQGASYGKWILIQHNNGLSTLYAHLSLIKIGEGQTVESGQLIGYTGNTGYSTGPHL
                     HFTVYASQGVQVGSLKSKVPGCGTYRLPIASYNSYLNPLNYL"
     gene            5796..6068
                     /locus_tag="PLH96_01270"
     CDS             5796..6068
                     /locus_tag="PLH96_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60434.1"
                     /translation="MNNFKNRKEAGLVKLIILIIVVILILSYLGINIQKIAESETGQA
                     NFGYLWNIVKVIWEYLTNFWNEYLKEPAMSIFGKIPLPPAVTEALK"
     gene            6466..6822
                     /locus_tag="PLH96_01275"
     CDS             6466..6822
                     /locus_tag="PLH96_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60435.1"
                     /translation="MAIERPENKAKSELYDEIVKTSVEYGIHPDTALSIAMCESSLRQ
                     YDSNGNSLRGIENSNDVGVFQINEKYHLEKSRELGYNINTVEGNVGYAMWLMKKEGTK
                     HWNYSKPCWSPKLAKL"
     gene            7811..8143
                     /locus_tag="PLH96_01280"
     CDS             7811..8143
                     /locus_tag="PLH96_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60436.1"
                     /translation="MREAEIRFTAERAWLKDDFGEFFFRRRDIVSDPRLTDTWQEIAL
                     GVRKPAPRYDCLWEAVREAASVQDHPHLVDIIVNDMKEKGLVANPNKMTPEDILWVMV
                     EWSLPWWD"
     gene            complement(8194..8601)
                     /locus_tag="PLH96_01285"
     CDS             complement(8194..8601)
                     /locus_tag="PLH96_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60437.1"
                     /translation="MNIIDKFTKKVKWLKETGESEFVKSEKPLFWALVIILTASLSFG
                     LGRLSKIDEVRAPVRVEKTPASAMVAVVGLVGGEQGSEVSEKLIVASKRGKKYHYLWC
                     SGAKTIKEENKIYFVTEVEAEQAGYVLAANCQK"
     gene            8722..8994
                     /gene="rpmA"
                     /locus_tag="PLH96_01290"
     CDS             8722..8994
                     /gene="rpmA"
                     /locus_tag="PLH96_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPB60438.1"
                     /translation="MAHRKAGGTAKNLRDSNPKYLGTKLHDGERAIAGNIIIRQRGTK
                     IVAGEGVAMGKDHTIFAKKEGKVKFSEKRKVSYDGQVKKVKVVSVD"
     gene            complement(8991..9434)
                     /gene="rplI"
                     /locus_tag="PLH96_01295"
     CDS             complement(8991..9434)
                     /gene="rplI"
                     /locus_tag="PLH96_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPB60439.1"
                     /translation="MKVILKRDVAKVGRKNEIKNVADGYARNSLIPQGLAVPATAENL
                     AKIEEWEKGKTAAGAKSDSVFAALRAKLTDQTIKTKQKAGPEGHLFAGLREKDVAGIL
                     AEETGVAVKPDWLLFSRPLKSIGEHKITLRQGESDFTCLLVIEKA"
     gene            complement(9447..10682)
                     /locus_tag="PLH96_01300"
     CDS             complement(9447..10682)
                     /locus_tag="PLH96_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPB60440.1"
                     /translation="MNKKLWLKLILVVVAICALFSAFLIGVYYGYEKRPEVEKVAGLV
                     NKEPGETVEVDFAPFWKAWNIVNEKYLMENGTTTEKVTNQEKVWGAISGLVMSLGDPY
                     TVFLPPAEKKRFEDDISGNFTGVGMEIGIKENVLTVVSPLPDSPANRAGIKAGDQIIK
                     IDDKITANMSTDEAIGLIRGEQGKPVHFVLLRAGNEKPIELTVIRDVITIPTLKTEKL
                     ESGIFVIRLYNFSAPSPELFRDALQEFADAKTDKLILDLRGNPGGYLDAAVNMASWFL
                     PVGKPVVIEKHSSGESDKIYRSKGYDVFNENLKMVILIDQGSASASEILAGALSEYDK
                     AILIGEKTFGKGSVQELVPLTQDSSIKITVAKWYTPLGHSISESGLEPNIKISITDED
                     LESGRDPQEAAAVDYLLKR"
     gene            10757..11695
                     /locus_tag="PLH96_01305"
     CDS             10757..11695
                     /locus_tag="PLH96_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPB60441.1"
                     /translation="MTKQIKYKSLIWLDLTNPTKEEIASLPDNFKVHPLIKEELRHPS
                     PRPKIDLYDEAIHLVLHFPNENSTNKNDSPNQTNHQEVDFVLGQNFLITAHYEEIQPL
                     EEFAKILEASEGWGRREGKNLHAGYLFYYIIRQLYESLEPGLHFINNNLKRAEQKVFG
                     GQQEKMVKTLADINRYLLDFRWTLKDHEEILQSLEAATKEFYGNTFGYHMRSITGDYH
                     KVMNSLENNEQSFKEIRAVNESMLSIKNNEIMKTLAVLAFIFLPATMIGTVFGMSGSD
                     LNMPLIKSEFGFWWALGLMVVTAVISFLFVKFKKWL"
     gene            11686..13095
                     /gene="cysS"
                     /locus_tag="PLH96_01310"
     CDS             11686..13095
                     /gene="cysS"
                     /locus_tag="PLH96_01310"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB60442.1"
                     /translation="MALVFYNSLSRRRDELSKTPGEAIVMYSCGPTVYDFAHLGNLRS
                     FIFADTLFRTLGYFGYKPSWAMNITDVDDKTIRGTINQKGLTAKPADLADYTKVFLTG
                     FIEDLEKLNIDPKQIKITRVTEIIPLIQEKIIRLIDTGFAYRAEDGSTYFSVNKYQEK
                     YGDYGQMVGENFLAGKKVGARVKQDDYTKENISDFALWKARSEDDGEIYWDHPILGQG
                     RPGWHIECSVVNDLSFKQKTIDFHTGGVDLAFPHHTNEMAQSRALNDAPLSHFWLHLG
                     LLLVEGQKMSKSLGNFVRLKDLPQNNASPLAYRYLVLQSGYGQTLNFTQESLRAAGEA
                     YRDLLLKTAWLLTQTNNTKGKILSAAKEEFMNALADNLNTGSALAVLWKVLSYTDTNP
                     ADQLATILDFDQVLGLDLKSAANLLDVPGEVRSLATQREKARAEKDWSTADQLREQIN
                     EQGFEISDTENGPFIFRQF"
     gene            13199..14557
                     /gene="dnaB"
                     /locus_tag="PLH96_01315"
     CDS             13199..14557
                     /gene="dnaB"
                     /locus_tag="PLH96_01315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPB60443.1"
                     /translation="MATKQTTVGYKLRVPPNDLEAEQALLGSVMLNPKGLAEVLDFLT
                     PEDFFAGRNQKIFAAMAKLFEKGEPVDLISVSAWLKENKQLESAGGSAYVAELSARVP
                     SASNLRYYADLVHRKSALRSLIEASEKISDLGYQEDREVEEVLDEAQKVIFNLGAFTN
                     KKVINLKDALGEAWERFDRLSKSDGAMRGVPTGFHDLDNKLAGLQKSDLIILAARPSV
                     GKTSLALDIARHAACREETPTCIFSLEMSSQQLTDRLLAAESFVDSWKLRTGAIRADE
                     EFARLRDALDTLAQAPIFIDDEPSNNIMRMKSEARKLKAEHGLGLIVVDYLQLMVPRR
                     ETDSQVQMVTEISRSLKALARELEVPVLALSQLSRAVEQRGGKPRLSDLRDSGSIEQD
                     ADVVMFIHREKNEDTNEKHDIAEILIEKHRNGPTGKIDLVFDGARTSFSNMEKGDFGA
                     FS"
     gene            14771..15397
                     /locus_tag="PLH96_01320"
     CDS             14771..15397
                     /locus_tag="PLH96_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPB60444.1"
                     /translation="MDPINTLTEIFRHFPGIGPRQAKRFVYYLLNQNPDSLKKLTEQI
                     LALKKNIRQCADCFRYFNNSYASPNNLCRICQDSSRDESSLMLVERDIDLEAVEKAGS
                     YRGYYFVLGGNIPILDKEPRKKIRENDLLKLIGQKGSRLQELIIATSANAEGDNTADY
                     LKSLLAEPAQKYHFKISLLGRGLSTGSELEYADPETIKNALNNRHSQN"
     gene            complement(15443..15751)
                     /gene="rplU"
                     /locus_tag="PLH96_01325"
     CDS             complement(15443..15751)
                     /gene="rplU"
                     /locus_tag="PLH96_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPB60445.1"
                     /translation="MFAIVETGGKQYKITEGDVLKVEKLPKPENGKIIFDKVLLINDG
                     QKTQIGAPYLEGATVTVDFMEEGRGKKITVIHYKAKVRYRKVYGHRQPFTKVKVGKVA
                     "
     gene            15936..>16202
                     /locus_tag="PLH96_01330"
     CDS             15936..>16202
                     /locus_tag="PLH96_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60446.1"
                     /translation="MVIEGFDLTTVKLTAEEERFVFRLTDTLSEIWGNFAGDLLARTQ
                     RLLEAEFGQASRSKRLEHLGLMQDAWEDGLLDNYQEPTPHKFAVF"
BASE COUNT         4607 a         3816 c         3450 g         4329 t
ORIGIN      
        1 ataaagaaaa agaggtaggc accccgcgaa tgggcggaat cgtcatctgg gggagcgtca
       61 tcgccaccac catcatcttt tggcttctac aaactgtttt cccgtcagaa gcatctagta
      121 aacttgactt cgtcagccgg aatcagactt ggttgccgct cttcacattg ttggtcggct
      181 ctatcgtcgg tttggttgac gacattttgc aggttcgccc aaccgaaaac tcggctaagg
      241 ccggcctgtc tttttataaa agactggcaa ttgttttagc gctcggcttg atcggtggct
      301 attggtttta ctttaaacta ggaatggaca gtgtcagcct gccctttatt ggagagtggc
      361 aaattggctt ttggttggtt cctttgtttg ttttagtgat gatcgcgctt ttctcaagcg
      421 gcgtcattga cgggttagac ggcttgtcgg gcggaattat ggcgattatt ttcgcggctt
      481 ataccggcat cgcttatttc caaagccaga ttgacttggc cgctttttgc gcggtggtca
      541 ccggcggaat tttggccttt ttgtggttta acattccgcc agccagattt tatatgtccg
      601 agacggggat tctgggcctg accaccactt tgactgttgt tgcgttcttg actgattctg
      661 tttttacttt accaattatc gccctgccac ttttgattac ttcactttcc gtaatcatcc
      721 aacttttatc caaaaaagtc cgtggccgta aagttttctt ggtcgccccg ctacatcacc
      781 actttgaagc aattggctgg ccggcttaca aagtggtaat gcgctactgg gtaatcggta
      841 tcatctgcgg cattatcggt atgattttga ctttaatcag caagttttag aatgaaaacc
      901 gccaaaaccg ccaaaccgga taggtttttg ttcagcctga ttctcatttt gataacaggc
      961 ggttttgtta ttttttcttc ggccgccttg gggcaagtcg gccgaaccag cgccagtttt
     1021 tccgtggtcg ctctaaaaca attgctagtt ctttttttgg gttttgttat gatgcttatc
     1081 gcttccaaaa ttccttacaa gcgttggcgc aaatattcac cttacatctt tgccttggcg
     1141 ctcgttttga ccagtttagt ttttgtgccc ggaataggat tcggaactaa cggcgcgaga
     1201 cgttggcttg acctgggttt tacaacattc caaccatcag aatttttgaa atttggctta
     1261 attgtttttt tagcggcctg gcttgccgcg cgcaaagaaa aaatcaggaa tttaaaagag
     1321 gggtttttgc ctttcttgat ttttttaagc atagtttgtt tgctcctgct taaacaaccg
     1381 gacaccggga cattaatagt agctatcttt gcggccttga gtttgtttat gatcgccggc
     1441 gggcgatggt cacaccttct tataattatc ctgttgggaa ttttgttggc cggcgcctta
     1501 atttggacca gaccttatgt taaagaccga ttggtaacct atcttgaccc gcaaaaagac
     1561 tcacggggcg cctcttggca aataaaccag tcgctgattg caatcggatc gggcggctta
     1621 gtcggccgcg gcttcggtca aagcatccaa aaatttaatt ttttgccaga accaatcggg
     1681 gactccattt ttgcggtcgc cgccgaagag tttggtttta tcggctccac catcattgtc
     1741 ggtctattct tgttattggc cttgtggggc ttaaagttga tgaacaaagt caaagactca
     1801 ttcggacgcc tgttgggtat tggcttggtc cttttgataa cggtccaatc gttcgtcaac
     1861 atcggcgcaa tgattggaat tttcccgctg accggcttgc ctttactttt tattagtcaa
     1921 ggcggctcag cgcttttctt tgctttaatt gaggttggta ttatactaaa catatcaaaa
     1981 gaagtaagaa aataaacatt ttattatgaa aatcttattc acaggaggag gcacaggcgg
     2041 tcatttttat ccggttattg ccgtcgctga agaagtaaaa agaatttgta ccgaacaaaa
     2101 aattgtcgcg cccgaactct attttatgtc tgacaaacca tacaacgaac gagcgctctt
     2161 agacaacggc ttaaaattcg tgcctgtcca agcggggaaa atcagacgct atttttcctt
     2221 gatgaacttt tttgacgttt ttaagaccat ttttggttca attagagcca tttttacgct
     2281 tttctccatt tacccggacg ttgttttcgg aaaagggggt tatgccagct ttccgggtct
     2341 gctggcggcc agacttttgc gaattccagt agtcatccac gaatctgatt ctcacccggg
     2401 tcgagtcact ctctgggccg gaaaatttgc caaacgcatc gcagtctctt acccggaagc
     2461 ggccaaatat ttcccaccag aaaaaacggc cgttgttggc aacccgatta gacgcgaagt
     2521 gctcttcccg gccaaagccg gcgcgcatga atttctaggt cttgataaaa acatcccaat
     2581 tatttttgtt gttggcggtt cgcaaggcgc cgtgcgtata aacgatgtcg ttttggatat
     2641 cttgccccaa ctgttggctg actaccaagt gattcatcaa gtggggaaaa gcaactatga
     2701 cgacttgaaa aaccgcgctt ctttcttgac tgaaaataac ccgcacaaaa atcgctacaa
     2761 actttttgat tacctgaacg acgtcgcgat gagaatgacg gccggcgccg ctaatttggt
     2821 catttcacgc gccggttcgg caatctttga aatcgctaat tggggtattc cggccattat
     2881 tatccctatt ccggaagaaa ttagccacga ccaacgcaca aacgctttta cttatgcccg
     2941 aagcggcggc gcggtcgtga ttgaagaaaa aaatttgact ccttccattc tcttgtcaga
     3001 aacaaaaaga attttagacg atcctgaatt gcaaaagcaa atgaaaaccg gcgcgcaaag
     3061 ttttgctaaa ccagaggccg gcgaaaaaat cgccgaggaa attattcgct tagccctgga
     3121 acatgaagaa tagggtcatc accagagtag caccgagccc gaccggttta atgcacattg
     3181 gcactgcccg ggtggctctt tttaatcacc tctttaccaa acaaaatgac ggcaaaataa
     3241 ttttacggat tgaagacacg gaccgtgaac gttccaagcc agagtttgaa aaaaatatca
     3301 aagacggttt ttcatggctg ggcctgactt ttgacgaaat ctggaaacaa tcagaacgaa
     3361 cggaaattta tacccaatat ctgcaaaaac ttttggccga agacctggct tatgtctccc
     3421 aagaagaagc caaaaaagaa ggggaccggt catcggtaat cagatttaga aacccgggtc
     3481 aaaaaattac ctttcaagat ttgattcacg gcgaaataac ttttgacacg acagagttgg
     3541 gcgatttcgt cattgccaaa gatttggaaa caccaatttt ccattttgcc aatgttgtag
     3601 acgacctaga agccggcatt actcacatca tccgagggga tgaccatatc tccaatacgc
     3661 cgcgacaaat tttaatcttc aaggctttgg gaggtcagat cccgatctat acccacttgc
     3721 cgatgatcct agcgcctgac aaatccaaat tatccaagcg ccacggtgct tggccagtga
     3781 ctgaataccg cgacgagggc tacctaaaag aagctgttct gaattttatc gttctgcttg
     3841 gttggtcacc tcaatcacgc gccgagattg cccaagacgg cacagaggat attgtttctt
     3901 tggaccaaat gcttaaactt tttgatttgg ccaaagtcca gaaaaaaggc gcggttttca
     3961 atttggaaaa attgaactgg ctaaaccgcg aacacctaaa gcggctgccc acaacagaac
     4021 aatggcaaaa aataatcgac tggctgccgc caaccaaagc tgactttatg aaaacgaatg
     4081 aactaatgct tcaaaaaatt acacccttaa ttttggaacg agtttccacc ctaaaagaag
     4141 ctcaggcttt gctggcaaat ggcgagtttg attacttctt ccatcagcca aaaccaagcc
     4201 aagagcttct gcgcaccacc actcatctga cagaaatcgc cagccggctt tcagaactgg
     4261 cagcggaaga ctttaccgcg gagacaatca aaaacaaaat atgggatttt gcgaccgaaa
     4321 agggcagggg agaggtcctc tggccactgc gcgtggcctt gtctggcaaa gaaaaatcac
     4381 ccgacccttt tgtcttggcc gcgatattag gccgcgctga aactttaaac agaatcaaaa
     4441 atgcgctttc ttagttacaa ttcacaaatc tcaatcattg tagttatcat tttgcttttc
     4501 agcggttttg cttggcgcga taacaaaagc gcggtccaag ccgccaccgc cgacgaatta
     4561 cgaaacaaaa ttgaaaacag ccaaagtgaa attcaaaagt tggaaaaaga aatccaaact
     4621 ttccgccaag aaataaccaa gacccaacaa gaaaaaaatt cccttaatac tgagataaaa
     4681 aaaattgact tgacccgaca aaaattaggc agcgaaatta aactaaccca aaatcaaatt
     4741 aagaccaccg aaaaacagat tgccgaattg gcaaacggta tccaagataa agaaacggcc
     4801 attgaacaca acagagcggt tatcgccgag tctatgcgtc ttttggcggg aacggatgat
     4861 gaaagtttgc ttgtttcctt tttggccagc gagagtttgt cagaattctg gaacagcgcc
     4921 gatcaactag cccaagttaa taaccgttta atcaaattga ttgccgaatt aaatcaaaac
     4981 aaaaaacaac tgacctccac taaggttcaa aaagaggttc agaaaaaaga tttgtctgtt
     5041 ttgacaacca aactgagcga ccagaaacaa ctggtggacc agaccaagca agaaaaaaac
     5101 agtctgcttt cggcaacgca aagtaaagaa accgcgtatc aaaaactcct ccaagaccgc
     5161 ctggccaaaa agcgcgccgt ggaagccgag atgcaacaag ccgaagccca actgaaagtc
     5221 gtcctggacc ccaaccgcct accggattca ggcaccaaag ccctccgctg gcctcttgat
     5281 aaaatcataa tcacccaata ctttggcaac acggcttttg ctctgcaaaa cgctgctgtt
     5341 tataacggca aaggccacaa cggcattgac ttggctgccg atgtcggaac cccgctcaag
     5401 gccgccaaca gcggtaaagt tatcggggtt ggcgacacgg ataacacttg ccaaggggct
     5461 tcctacggca aatggatttt gattcaacat aataatggcc tctcaactct ctacgcgcac
     5521 ttgtcattaa ttaaaatagg cgagggtcag acagtggaaa gcggacagtt aatcggctac
     5581 accggtaaca ccggttattc caccggcccg cacctgcact ttaccgttta cgccagccag
     5641 ggtgtccaag ttggttcact caaaagcaag gttcccggtt gtggcactta ccgtttacca
     5701 atcgcttctt acaacagcta tcttaatccg ctcaactatc tctaatttgt gttataattt
     5761 agctaattgg cattttttat tcttctatta aaactatgaa caatttcaaa aatagaaagg
     5821 aggccggttt ggttaagttg attattttaa ttatcgtcgt tattttaatt ttgagttatt
     5881 taggcataaa tatccaaaaa atcgccgagt cagaaaccgg tcaagccaat tttggttatc
     5941 tttggaacat cgtaaaagtg atttgggagt atttaacgaa cttttggaac gaatatctta
     6001 aagagccagc aatgtccata ttcggcaaaa tcccattacc gccggcagta acagaagcct
     6061 taaaataggc catattttgg tatttcaatt ttcctttgta aacaaagact ttttttggcc
     6121 cgctccccga aaaacgttgc aaaataaggg ttttttggcc aaggactggc ttctgacagt
     6181 ccgtttttga ttgactttca agctcaacta tgctaagcta aacttgctct tgtaaaagag
     6241 taaatacagt tgttagttgt tagtcttgaa agtatgaact gtagagtcga caattagtta
     6301 agaaataaac aggctacgta ttatgaaaag taataggtag tttttatttg acttatttta
     6361 agaaccctac aaataggctt agtaagcctc atagcgacta atggtcttaa tccgaccgct
     6421 gaactaatta acgctgacga gcctccggag ccccccgcca tcgtggtggc catagaaaga
     6481 ccggagaaca aagccaagtc agaattgtac gacgagattg taaaaactag tgttgagtac
     6541 ggaattcatc cggacacagc cctcagtatc gccatgtgcg aaagcagcct tcgtcaatat
     6601 gacagcaatg gtaattcctt gcgcggtatc gaaaactcca atgatgtagg tgtttttcaa
     6661 atcaacgaaa aataccactt ggaaaaaagc cgtgaattgg gttacaacat caacactgta
     6721 gagggtaatg ttggttacgc catgtggttg atgaaaaaag aggggaccaa acactggaat
     6781 tatagtaaac cgtgctggag tcctaagttg gctaaactct aaaaacaacg ttaagaaaca
     6841 aaaagctccg ttctaaacgg ggctttttgt ttgcctctac cccctacgcc cgtgacaaaa
     6901 gttaaaggaa ctgtttcgat attttgcgac attatgccag gcttggcggg tatgcccact
     6961 taaaataaaa aataaaaaaa cagccggtta aaaattattc actctcaaga aacctgagaa
     7021 agtccgaggg acaaaaaatg atgggccaaa attcgagtaa gttatcaact tataaaatta
     7081 ttggcgtcga tttaaaaaaa catttaatct cgattaaaaa tagaagtcat cttaagttgt
     7141 tttcaaaatt caacccatca gcaaaaccaa accgccacaa aaataataaa aaataaaaat
     7201 gggttcaata agcaaatttc agatagccaa aggtattgct caaaaattaa cggggccttc
     7261 tagagcaatt ttggggctcg tttagaagcc atatttatgt gattcaagtc atttctttgg
     7321 attgacgatt tttgcgagat tctacaaaag acacattaaa agacacatta aaaaaccaaa
     7381 aaaagtgagg ccaaacttaa agaagcaagg cgcacgccga aattaaaaaa taaagtaaaa
     7441 taaaatacca aaataaaaaa tagcagcaga ggaggagagg caagagaaaa tttagagggc
     7501 aaagcggtgg agccttattt taataggttt ttaaatatat tatgtcgcaa aatctgtgtt
     7561 gtgcgacatt gctaaagtta tccacaggcc cttccctaca aagccggtga aacttttttg
     7621 acaagaggaa ttttcttctc tctggaaaaa ccaccttgag caacgtctgg cggcgaccga
     7681 cagaccggag aaccttttca ccggctacta ttggctggtt tcgcccgagc taaacgcttg
     7741 ggaaattaac gaggaaggga aggtcatcgc ctgcgcttcc gcttcccgaa cggcgaaggc
     7801 ccaccccatt ttgcgagagg cagaaattcg cttcacggcg gaacgggcct ggctgaagga
     7861 cgacttcggc gaattctttt tccgacgccg ggacatcgtt agcgatccaa ggctaactga
     7921 tacctggcag gaaatcgccc ttggcgtccg caagccggcc ccaaggtatg actgcctttg
     7981 ggaagccgtg cgtgaagcgg cttcggtcca agaccacccc caccttgttg acataattgt
     8041 caacgacatg aaggagaaag ggctggtcgc caacccaaac aaaatgaccc cggaggatat
     8101 cctctgggtc atggtggaat ggtcactccc ttggtgggat tgattcttcc gcttgagccc
     8161 cgcttttgcg gggtttttta taccaacccg aaactatttc tgacagttag cagccaaaac
     8221 atagccagcc tgttcagctt caacttcagt cacgaaatag attttatttt cttctttaat
     8281 ggttttggca cccgaacacc ataggtaatg atattttttg ccgcgtttgg aggccacaat
     8341 caacttttcc gatacttcgc tcccctgctc tcctccgacc aaaccgacga ccgccaccat
     8401 tgctgaagca ggcgtttttt ccactctgac tggcgcgcga acttcatcta ttttagataa
     8461 acgtcctaag ccaaaagaca aggaggccgt caagattatg accaaagccc aaaacagcgg
     8521 tttttccgat ttgacaaact cgctttcgcc tgtttctttc aaccacttta cttttttagt
     8581 aaatttatct ataatgttca tggtaaaatt ggttcaagag taaagcctaa aactggcttt
     8641 tttaaattat agcgactttt ataacttttc ccaatcttta cccgccttat tttaagaata
     8701 aaaatttaac aaacaaaagt tatggcacat agaaaagcgg gcggtaccgc taaaaacctg
     8761 agagattcca atccaaaata cctgggcacc aaactccacg atggcgagcg cgcgattgcc
     8821 ggcaatatta ttattcgcca acgcggcacc aaaattgtcg ccggcgaagg cgtagcgatg
     8881 ggcaaagacc ataccatctt tgctaaaaaa gaaggtaagg taaaattttc cgaaaagcgc
     8941 aaagtcagtt acgacggcca agtcaaaaaa gtaaaggttg tttcggttga ctaggctttt
     9001 tcgattacca acagacaagt gaaatcactc tccccttggc ggagagtgat tttgtgttcc
     9061 ccgattgatt tcaatggtct ggaaaacaac aaccagtctg gtttgaccgc gacacctgtt
     9121 tcttcagcca aaatccccgc cacgtccttt tcacgcaaac cggcaaaaag gtgaccttcc
     9181 ggcccagcct tttgttttgt cttaatggtt tgatcagtta atttggcgcg caaagcggca
     9241 aagaccgaat cacttttggc accggcggcc gtttttcctt tttcccattc ttcaatcttg
     9301 gctaaatttt cggcggtagc cggaaccgcc aaaccttgcg ggatcaagct gtttctggca
     9361 tagccatcgg ccacgttttt gatttcgttt ttgcgaccga ctttcgcgac atctcttttt
     9421 aaaataactt tcatttaaaa tttaaattaa cgtttaagca aatagtcaac cgccgccgct
     9481 tcttgcgggt cgcgacccga ctctaaatct tcgtcagtga tggatatttt aatattcggt
     9541 tcaagaccgc tctcggaaat ggaatgaccc agaggagtat accatttcgc caccgtaatt
     9601 ttgattgatg agtcttgagt caagggcacc aactcttgca ccgaaccttt gccgaaagtt
     9661 ttttccccaa tcaaaatcgc tttgtcatat tccgacaaag cgccggccaa aatctcggaa
     9721 gctgaggcgc taccctgatc tatcagaata accattttta aattctcatt gaacacgtca
     9781 tagcccttac tgcggtaaat cttgtcactt tcgccgctgc tatgcttttc gatcaccacc
     9841 ggtttaccca ccggcaaaaa ccaactcgcc atattgaccg ccgcgtccaa atagccgccc
     9901 gggttgccgc gcaaatcaag aattaattta tcagttttgg catcggcaaa ttcctgcaaa
     9961 gcgtcacgga acagttccgg cgacggcgcg gaaaaattgt aaagcctgat gacaaaaatg
    10021 ccgctctcca atttctctgt ttttaaagtc gggatagtaa tcacgtcgcg aataacagtc
    10081 aactcaatcg gcttttcgtt ccctgcgcgc aacagtacaa aatgcaccgg cttgccttgt
    10141 tccccgcgaa tcaaaccaat cgcctcgtcc gtgctcatgt tggcggttat cttgtcatca
    10201 attttgataa tctggtctcc agccttaatc cccgcccgat tagccggact atccggcaaa
    10261 ggcgaaacaa ccgtcaaaac attttctttg ataccaatct ccattcccac gccggtaaag
    10321 ttgccgctaa tgtcgtcttc aaaacgtttc ttttcagccg gaggcaaaaa gacagtataa
    10381 ggatccccca aagacataac caagccgcta atcgcccccc agactttttc ttggttggtg
    10441 accttttcgg tcgtagtgcc attttccatc agatactttt cattaacaat gttccaagct
    10501 ttccaaaacg gcgcgaaatc cacttcaacg gtttcgcctg gctctttgtt gactagaccg
    10561 gccacctttt ccacttccgg tcttttttcg tagccataat aaaccccaat taaaaaagcg
    10621 ctaaacaaag cacaaatagc aaccaccacc aaaatcaatt tcaaccagag ttttttgttc
    10681 attttcgtat tttcgtttta attatcgcaa aatatccact tgaggtcaaa ctcaacaaag
    10741 gtatataata ggcttaatga ctaagcaaat caaatacaaa agcctgattt ggctggactt
    10801 gacaaaccca accaaagaag agattgcctc tttgccagac aacttcaagg ttcaccctct
    10861 tattaaggaa gaattgcgcc atccctcgcc acggcccaaa atagacctct acgatgaagc
    10921 aattcatttg gtcttacact tccccaatga aaattcaacc aacaaaaatg acagtcctaa
    10981 ccagaccaac catcaagagg tagattttgt tttgggacaa aactttctga ttaccgcgca
    11041 ctatgaagaa attcagccct tagaggaatt cgccaaaatc ctggaagcga gcgagggttg
    11101 gggtcggcgt gaaggcaaaa acttgcatgc tggttatctt ttctattata ttatacgcca
    11161 actttatgaa tcgttggaac ccggtctcca cttcataaac aacaacttaa aacgcgctga
    11221 acaaaaagtt tttggtggtc agcaagaaaa gatggtcaaa accttggccg atatcaatcg
    11281 gtatcttttg gattttcgtt ggaccctaaa agaccacgaa gaaattttgc aatcattaga
    11341 agcagccacc aaagaattct atggtaacac ttttggttat cacatgagat caatcaccgg
    11401 cgattaccac aaagtgatga acagcctgga aaacaacgag caatccttca aggaaattcg
    11461 cgccgtcaac gaatctatgt taagcatcaa aaacaacgag attatgaaga cattggcggt
    11521 gctggccttt atctttttac cggccacgat gattggcacc gttttcggca tgagcgggag
    11581 tgatttgaat atgccattga ttaaaagcga gtttggtttt tggtgggccc tcggcttgat
    11641 ggtcgtcacc gccgtcattt catttttgtt tgttaaattt aaaaaatggc tctagttttt
    11701 tataacagtc tcagccgccg gcgtgatgaa ttatccaaga cgccgggcga agccatcgta
    11761 atgtattctt gcgggccaac ggtttacgat tttgcccatc ttggcaactt gcgcagtttc
    11821 atttttgccg atacgctttt tcggaccctc ggctattttg gctacaaacc aagttgggca
    11881 atgaacatta ccgatgttga cgacaaaaca atccgcggca caatcaacca aaaaggtttg
    11941 acggcgaagc cagccgattt ggcagactac actaaggttt ttttgactgg ttttattgaa
    12001 gatcttgaaa aactcaacat tgaccccaaa caaattaaaa tcaccagagt gaccgagata
    12061 attcctctca tccaagaaaa aattatcaga ctgatagaca cgggttttgc ttatcgcgcc
    12121 gaagatggca gcacctattt tagcgttaat aagtaccaag aaaaatacgg cgattatggc
    12181 caaatggtcg gtgaaaattt tttagccggc aaaaaagttg gggccagagt aaaacaagat
    12241 gattacacca aagaaaatat cagcgacttc gctctctgga aggctcgcag tgaagacgac
    12301 ggcgagattt attgggacca tcccatttta ggtcaaggtc gtccaggttg gcatattgaa
    12361 tgttcggtcg tgaacgacct gtcttttaaa caaaaaacaa ttgacttcca caccgggggc
    12421 gtggatttgg ctttccccca ccacaccaat gaaatggccc aatcaagagc cttaaatgac
    12481 gctccgcttt ctcatttttg gctccacctt ggtctcctct tggtggaggg acaaaaaatg
    12541 agcaagtctt tgggcaactt tgttcgctta aaagatttgc ctcaaaataa cgcctcgcct
    12601 ctcgcctatc gctatcttgt tttgcaatcc ggttatggcc aaacactcaa cttcacccag
    12661 gaaagtttga gggcggccgg ggaagcttat cgcgacttgc ttttaaagac agcttggtta
    12721 ttaacgcaaa caaataacac caaaggaaaa attttgtcag ccgccaaaga agagtttatg
    12781 aacgccttgg cagacaactt gaataccggt tccgccctgg ctgttctctg gaaagttctg
    12841 agttataccg acaccaaccc ggccgaccaa ctggccacca ttttggattt tgaccaagtc
    12901 ctcggtcttg atttgaaaag cgccgccaat ctgcttgacg tccccgggga agttcgttca
    12961 ctagctaccc aacgcgaaaa agcgcgcgcc gaaaaagatt ggtcaacggc cgaccaactt
    13021 cgcgagcaaa tcaacgagca agggtttgaa atttccgaca ccgaaaacgg tccgttcatc
    13081 tttcgtcaat tttaacggga ttttcctctt tttaatcaca ttttgtcccc aaaatgatgg
    13141 tttgggacgt tttttaggtg ttttcctttc gcccctcagt ttgctaaaat aaagaataat
    13201 ggcaaccaaa caaacaactg tcggatacaa actacgcgtt ccgccaaacg acctagaagc
    13261 ggagcaagcg ttgcttggct cggtgatgct aaaccccaaa ggtctggcgg aagttttgga
    13321 ctttctaacc ccggaagatt ttttcgccgg ccgaaatcaa aaaatttttg ccgccatggc
    13381 caaactgttt gaaaagggcg aaccggttga tttgatttcc gtttcggcct ggctaaaaga
    13441 aaacaaacaa ttggaatcgg caggcgggtc ggcctatgtc gccgaacttt ccgctcgtgt
    13501 cccctccgcc tccaatctcc gttattatgc cgacttggtt caccgcaaat cggccctgcg
    13561 cagtctgatt gaagcttcag aaaaaatttc tgaccttggc taccaagaag accgcgaggt
    13621 ggaagaagtt ttagatgaag cgcaaaaagt tattttcaac ctgggcgcct tcactaataa
    13681 gaaagttatt aacctaaaag acgccttggg cgaagcctgg gagcgatttg accgcttgag
    13741 caaatcagac ggcgcgatgc gcggcgtccc gaccggtttt catgacttgg acaacaaact
    13801 ggcgggcttg caaaaatccg acctaatcat tttggcggcg cgcccatcag tcggtaaaac
    13861 ttctctggcg ctggatatcg ctcgccacgc cgcctgccgt gaagaaactc cgacctgtat
    13921 tttctcgttg gaaatgagct cgcaacaact aaccgaccgt ttgttggccg ccgaaagctt
    13981 tgtggactcc tggaaactgc gcactggcgc tattcgggca gacgaagaat tcgctcgtct
    14041 gcgtgacgct ttggacactc tcgctcaagc gccaatcttt attgacgacg aaccgtccaa
    14101 caacatcatg agaatgaaat ccgaggcgcg caaactgaaa gccgaacatg gcttgggtct
    14161 gattgtagtt gattatcttc aactgatggt cccccgccgc gaaaccgatt cgcaagtcca
    14221 gatggtaacc gaaatttccc gctcgctcaa agctttggcg cgcgaactgg aggtgccggt
    14281 gctcgctctt tcccagctat ctcgcgccgt agaacaacgt ggcggcaaac cgcgcctatc
    14341 cgacttacga gactccggtt ctattgaaca agacgccgat gtcgtgatgt ttatccaccg
    14401 tgaaaaaaat gaggacacca atgaaaaaca tgatatcgcc gagattttga ttgaaaaaca
    14461 ccgtaatggt ccgactggca aaattgactt ggtctttgat ggcgcgcgca ccagtttctc
    14521 caatatggaa aagggcgatt ttggagcatt cagctagaaa ttagaaagtt gtaagtcaaa
    14581 agttaaaagc tagaagttaa tgtaaaaagc taataagttt taagaacaaa aaatattaaa
    14641 ggaatttctt ttttctcttc gttttcttac ttttgactta taacttttga cttttaattt
    14701 ccctattaaa tacttatttt ctctttaatc ttaaactttt gacttttaac ttctaacctt
    14761 taacttaccc atggacccca ttaacacctt aaccgaaata ttccgccact tccccggcat
    14821 tggccccaga caggccaagc gttttgtcta ttacctgctc aaccagaacc cggactcgct
    14881 taaaaaatta actgaacaaa tcttagcgct gaaaaaaaat attcgtcagt gcgctgactg
    14941 ttttcgctac ttcaataaca gctacgcctc gcctaataat ctctgccgaa tctgccaaga
    15001 cagctcccgc gatgaaagca gtttgatgtt ggtggaacgc gacatagacc tggaggcggt
    15061 ggaaaaagcc ggttcctatc ggggttatta ttttgtctta ggcggcaaca ttcccatctt
    15121 ggacaaagaa ccgcgcaaaa aaatccgcga aaacgacctg cttaaactca tcggtcaaaa
    15181 aggttcccgc ttgcaagaac tgattatcgc cactagcgcc aacgcggaag gtgacaacac
    15241 cgctgattat ctgaaaagtc tgctggccga accggcgcaa aaatatcact tcaaaatctc
    15301 cctgctgggc cgcggtctct ccaccggcag cgagctggaa tacgccgacc cggaaaccat
    15361 caaaaacgct ttaaacaatc gtcattcaca aaattaaccg ctccccgaga gcggttaatt
    15421 ttgtgaactt taattttggg agttaagcga ccttgccaac tttcactttg gtaaagggct
    15481 gacgatgacc gtaaactttt cggtaacgaa ccttggcttt gtagtgaata acggttattt
    15541 ttttccctcg gccctcttcc ataaaatcaa cagtaactgt cgccccctcc aagtaaggcg
    15601 cgccgatttg agtcttttga ccatcgttta tcaacaaaac tttatcaaaa ataatcttgc
    15661 cgttttcggg tttcggcaat ttctccactt tcaggacgtc gccctcagtg attttgtatt
    15721 gttttccgcc tgtttcaacg attgcaaaca taatgggttt tattataccg gaatctttaa
    15781 aaaaggcaag tttttagggg tattttgtta ataagtggcc aaaagcttgc tttttttacc
    15841 tgtttatgat ataatgatag ataggtgcaa tagttctcta aatatttggg tttagcttgc
    15901 tccggtcaag taatcggagc tgggaggggt aaatagtggt tatagaaggt tttgacctca
    15961 ccaccgtcaa actgacggcg gaagaggaaa gatttgtctt ccgattgacc gacaccctgt
    16021 cggaaatctg gggaaacttt gcgggcgatc tgcttgcaag aactcaacgc ctcttagagg
    16081 cagagttcgg acaagcaagc cgaagcaagc gtcttgagca tcttggattg atgcaagacg
    16141 cctgggagga tgggttactt gataactatc aagaacccac cccacacaag ttcgccgtct
    16201 tc
//
LOCUS       DAOOLO010000012        15255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_4147_length_15255_cov_6.633224, whole genome shotgun sequence.
ACCESSION   DAOOLO010000012 DAOOLO010000000
VERSION     DAOOLO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4147_length_15255_cov_6.633224"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="miaA"
                     /locus_tag="PLH96_01335"
     CDS             complement(<1..561)
                     /gene="miaA"
                     /locus_tag="PLH96_01335"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPB60447.1"
                     /translation="MSEKPKIVVILGPTASGKTALSIKVAQKIGGEVISADSRQVYKG
                     LDLGTGKVTKKEMGSVPHHLLDVASPKRKFSVARYQKLANKKITEIIKRGRTPIIVGG
                     SGFYIQAIVDNLILPDVPPNPKLRLELQGKTIHELFSILKKIDPQRAKNIDENNPVRL
                     IRAIEIGKALGQVPKLQFANPPFNFIQ"
     gene            complement(677..758)
                     /locus_tag="PLH96_01340"
     tRNA            complement(677..758)
                     /locus_tag="PLH96_01340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(722..724),aa:Leu,seq:caa)
     gene            828..1835
                     /locus_tag="PLH96_01345"
     CDS             828..1835
                     /locus_tag="PLH96_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPB60448.1"
                     /translation="MPGNYEDKIFLIEVEKIKPNPFQPRSEFNNERLRDLADSIRQYG
                     VLQPLVVTRNEVEREDGSLYSEYELIAGERRWRASQLAGLKQVPAVIRTGAQSDKEKL
                     EMAIIENLQREDLNPIERAQAFFRLIKEFNLTHAEVAKRIGRSREYVSNTVRILNLPQ
                     EMLDALVSGKINEGHTRPLLMLSDRPHEQVTIFKEIIFKRLNVRDTENIARRIAFDKV
                     RKIETLVEPEILELEEQLTEKYGNRVTVEKKEKGGRVMIDWASEDDLKRILKMMCEVK
                     PQSILEVKDEMGSQAEVSEKEEQETENQAAELTASEKELPTEGPKDDEENLYFIKNFS
                     V"
     gene            complement(1832..3304)
                     /locus_tag="PLH96_01350"
     CDS             complement(1832..3304)
                     /locus_tag="PLH96_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPB60449.1"
                     /translation="MDIEEIFKLIKNLSPSDRDLITRAFAFAQKAHKGQKRYSGEDYI
                     VHPFNVALLLAEFETDAETIAAGLLHDTIENSDISAKELEKEFGPAVAFLVEGVTKLG
                     KLKYQGAKRHSESLRKLFVAMAEDIRVIMIRLADRLHNVRTLQYVPKEKQKRIALETI
                     ELYAPLANRLGVWKMKGRLEDASFPFAYPEDYQKVVDLRKTKGKETVKRLQKVYRVLQ
                     KAITEKGLKDFEIDYRLKYLYSLYTKLLRKNMDINDVYDISALRVVVRTTEECYQVLG
                     IVHSLWRPITGRLKDYIANPKPNGYQSIHTAVFIGDGTIVEIQIRTKQMQHEAEYGIA
                     SHVVYDEKSKKNKTAKASKNIQWIQDLINWQKEIKDGEKEKFVEELRRNFFHDRIFVF
                     TPKGEVVELPESATPIDFAYAIHSDIGNHASGAKINDKMSHLDAPLANGDLVEILTNK
                     NSGPRAKWLNFVKTTDAKRHIRAYLHLKRQKGEVAFNSLS"
     gene            complement(3370..5538)
                     /gene="topA"
                     /locus_tag="PLH96_01355"
     CDS             complement(3370..5538)
                     /gene="topA"
                     /locus_tag="PLH96_01355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB60450.1"
                     /translation="MKLLIVESPAKAKTISKYLDNAYTVKASVGHIRDLPKSNKNAID
                     IENGFAPNYVISPGKEKVVSDLKQLAKKADEIVLATDPDREGEAIAWHVKEAIGLKNP
                     KRIVFHEITEQAVKEAIKHPRAIDENLRLAQEARRVLDRLFGYDLSALIWQKVRYGLS
                     AGRVQSPALRILVEREKEIRAFVPENFWVISANFQTKNGQVLPFECREEPKTAERAEE
                     IVTAAREAVWQIANVTETEAKRSPRAPFTTSTLQQAASSRFGYAPSVTMRLAQKLYEA
                     GHITYMRTDSTNLSKTAVSEIILTINKEFGSNYASPRTYKTKSKNAQEAHEAIRPTHT
                     SLKSAGGNPQEKKLYQLIWQRAIASQMADAKILRTKVSAEADDQKVPPFETNGSQVLF
                     DGWLKADPAARGEDVELPKLTEKETLVLKEIEAVGKETEPPHRYTEAGLVKELEKRGI
                     GRPSTYAAIIKTIQDRGYAIKENKSLVPTDTGEVVSDFLTKYFNNYISDSFTAGMENE
                     LDDIADGKREYVKTLKNFYGPFTKEIANKKKIEKITNLGPAPDNAKCPVCGGPMIIKL
                     AKNGKFYSCAQFPNCTGALTLEGKELAGPKETGEKCPQCESPLVERDGRFGRFVACSN
                     YPKCRYIKKDAVIEGEDRPGDTGVKCPVCKKGTMVEKRGRFGTFYGCSNYPKCKNIIK
                     SKPTGGICPDCGSLMMQGTKTIPDRCSNKACSNHRPDKKK"
     gene            complement(5644..6516)
                     /gene="dprA"
                     /locus_tag="PLH96_01360"
     CDS             complement(5644..6516)
                     /gene="dprA"
                     /locus_tag="PLH96_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPB60451.1"
                     /translation="MDWLIKKIPRQEIPGPLQEIPQPPKSLYLVGELADCKKEYLLCV
                     VGSRKYSSYGREVVEKIIFGLGGQPITIVSGLALGIDSLAHQAALQVGLKTLALPGSG
                     LDQKVLYPSANRRLAEKIVAAGGGLLSEYEPNQAATLYTFPQRNRLMAGLSKATLIIE
                     AGAKSGTLITARLATEYNRDVLAVPGSIFSPNSHGPNWLIKQGAIPVTSAEDVLVALG
                     LQTQEDNKVDLNFSDLSPDEKIILELLQIESLDKEALIAKSGLKPSQANITLSMMEIR
                     GLIKEAMGEIHLKN"
     gene            6596..7858
                     /gene="serS"
                     /locus_tag="PLH96_01365"
     CDS             6596..7858
                     /gene="serS"
                     /locus_tag="PLH96_01365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPB60452.1"
                     /translation="MLDIKFIRENKAIIKEAARKKLLDFDVEALLEADNQRLKLVGEV
                     EKLRARQNEASHRIAKVKDEAERDELIEQMKDVKTLREGKEEELKQVLETWKSLLLKV
                     PNIPDISVPEGKDDSENQQIKIWGDRPKFEFAPRGHVELMTNLKLADFERGVKVAGFR
                     GYFLKNEAVLLSMAAWQHAMKIALEGGFVPLLTPVLDRPENFMGTGYLPDGEEDLYKT
                     QDGDYLAGTAEVPTMGYHFDEIVPADRLPYKFIAFSPCYRREAGSHGKDTQGLIRVHE
                     FYKVEQVVLCEASHEESVNWHEKLLANAERFMQDLKIPYRVVVNCTGDLGQGQVKKYD
                     IEAWVPSEGKFRETHSISYFHDFQARRLNTRYRDSEGKLRFVHSLNGTLVASPRILVS
                     LLENYQQADGSVVVPEVLRPYLGKDVIK"
     gene            7865..8737
                     /locus_tag="PLH96_01370"
     CDS             7865..8737
                     /locus_tag="PLH96_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60453.1"
                     /translation="MAAPKSKRDILRRSKEREQKMRKFRLGGGFLLVVIIVGGFLGLL
                     NWNFFCFNTVIVAGETNENKEAIQTYVLESIAGRYLGVVPKDSVFFLRKKILAEAVKH
                     HFPRLKDVQVSLPELNTLKISVEDKEAKLVWCDGQDGKKSGHCYYLNEDGEVYSEAPN
                     FSEAVMTEIIAPLPELPIGKIVLASSTVEKIQVLASAGGRLLNGLPGGYAPDLVSWQP
                     LTAGDWAGRVADREGRVWRILFNEKSKAADLVGGLGSILKNETFLTDWSRQKGRLEYI
                     DLRFPQKIFYRFIQ"
     gene            8772..9164
                     /locus_tag="PLH96_01375"
     CDS             8772..9164
                     /locus_tag="PLH96_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60454.1"
                     /translation="MNITLVISHYFFWHYSAALVRFTAIYKDIIFFVADFFSLPILLK
                     SFFAPWRRLAEPYPEDKLDFSEMMSVFIVNSLVRLVGVVMRSIMILVGLIALVLTVVL
                     YPVLVLLWLALPLVIVVLLAVGLKLLFF"
     gene            9168..11630
                     /locus_tag="PLH96_01380"
     CDS             9168..11630
                     /locus_tag="PLH96_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB60455.1"
                     /translation="MANKLQTYLRASRLYPVLVLDSLIAKRKRDWLINITGTLILLLL
                     LPILSYQIFSSLLSSSIFDIWSLLVPRFWGAIFVCSALYFLAKSLTFYFNDSYYFDNV
                     ALNKYANEDLFSITVGRVLYQADKGDALRGFFRSQTGQEILWRLGVDEEEQKKFLATR
                     QTVSDLPEQPLNGVLKMRALTQFLYDKNPDFALFLKKFGVGEKDLLGATDWVVNQIET
                     NEFVKRWWSKENLKRIPGLAKDWGFGSTFTLDKYGWDMLRGARYSNSSYEYSLFAAEV
                     SQLENILAKEKEANAILVGDSREEALDVVWHLIRKIQNRTAASALEHKRPILFNASVF
                     LSHFKERQGVEKELLKILDEARKAGNIILVIDDIYNLMTGFQNLGSSFLGLAEEYLQN
                     GALQIIAVTTTDAYHRFLAGNSGLMNAFDRIFVKTMSSESLVRGLQETVWQVEAKHKL
                     FFTYQSLHEIILSADSYFSESASSDKSVDLMTEMVSWAQNQGLKIIRRGDVQKFISSK
                     TNVPLGEIGPEERTKLQNLEVEIHKRVIGQDEAIKSVSGALRRSRAGVRNLNRPIGSF
                     LFLGPTGVGKTETAKALAEVVFGQESSLLRLDMSEFQNEDALTRLIGSFETGKTGILV
                     DMLREHPYAVLLLDEFEKTNREVLNLFLQILDEGLFSDMSGKRVNARNIIFVVTSNAG
                     AELIWENFRQGKKEENTSDNLINHIVAKGIFRPELLNRFDATVVFEPLSPESLRDIAR
                     LMLKKLGKRLAGQGLELVVSDYLTETVATLGANEVFGARPMQRFIQDNIEQKVADALI
                     DGRLVQGDKVVFQEPNLELTNA"
     gene            11623..13041
                     /locus_tag="PLH96_01385"
     CDS             11623..13041
                     /locus_tag="PLH96_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPB60456.1"
                     /translation="MPKMERSGGKSGKFSFQVVIALAIILILGFILFRVSQFLYAGVF
                     GNEAVSYQAGAASAGSVLKSDYTDFKVGATPVPKLGKSVIIDVKNQELHLFEKGELVK
                     SFSLLAIPESGSAWETSGGGFSVIKKEENHLSPITGLEFPYTVQFFGNSLIHGEPMDI
                     KGRIAKTAAGGFRLKNDDALTVFDWVDFDTKVLVYNARWTMGGNNHFSYIIKKSQPAP
                     LTAKSYLVSDLKTKEVILSKNKDKAVPIASVTKLMTALVALERLEPKTEIEVSRQAYE
                     TYGDSGYFRTGDKLPLEIMLYPLLLESSNDAAEAIAEKVGREKFIVEMNKKAKELGME
                     ETFFEDPSGLSPNNVSSAGDLVRLADYIHQNKIFLFDITKLKEYQHGRFVWTNRNNLI
                     GMNYYAGGKNGYTDEANRTLLALYDLPLSEFETRKIVLVLLGSDDRKSDTRNIVNYIA
                     GNVSYSGPSVFKTLDDFGWDEI"
     gene            13051..14061
                     /locus_tag="PLH96_01390"
     CDS             13051..14061
                     /locus_tag="PLH96_01390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPB60457.1"
                     /translation="MNLAKKLLPLLGGLVVGLSLIFWFFCFVSRQDVFFNEEVKLNPS
                     FKSEVETGEVTLLAVGDIMLDRGVAQLVNKKGGGDFAYVFENIRALKGEADILLGNLE
                     GPVSDRGADVGNLYSFRMSPKVLPVLKGVGFDILNVANNHAGDWGVAAFLDSLERLSA
                     AGLAYIGGGLTKVQAEKPVIIEKNGLKVGFLGFSDVGPNWLAATEREAGILLAGDIHF
                     SEIITQAAGQTDVLIVMIHFGEEYQAFHNERQENLAKTALQAGARAVVGHHPHVVQDE
                     ENLGHQYIAYSLGNFIFDQNFSVETMSGLALKLKLSQDGVAGVEKLPISINHNFQPNF
                     AN"
     gene            14063..15082
                     /locus_tag="PLH96_01395"
     CDS             14063..15082
                     /locus_tag="PLH96_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPB60458.1"
                     /translation="MKSFWGKLNKPFFALAPMYDVTDAPFRAMFAKYSKKAKTKEAPD
                     LVLFTEFVSCDGLASEEGRSKLLRELYYTEAERPIVAQVFGARPGNMEKAARLISELG
                     FDGLDLNMGCPDKAIEKQGAGAALLKDPILAREVVAAAKQGAGDLPVSVKIRLGYNKL
                     DLVWLEKIIATKPAALAVHLRTRKEMSLAPARWSDFPPIVTLAHEAGLVVVGNGDVVE
                     IADGQQKANASGADGIMIGRGAFGRPWLFSGGEEPALKEKLKILQEQTELFWDLYGPT
                     ETNKKLFAGHQKNFAVMKKHFKAYVSGFAGASNLRAKLMAAENPEAVKKIIREAEKEG
                     LGCLV"
BASE COUNT         3933 a         3024 c         3804 g         4494 t
ORIGIN      
        1 ttgaataaaa ttaaaaggag gattggcaaa ttgtaattta ggcacttgac caagcgcctt
       61 gccaatttca atggcgcgaa tgaggcgaac cgggttattt tcatctatgt tttttgctct
      121 ttggggatct attttcttta agattgaaaa aagttcgtgg attgttttgc cctgtaattc
      181 taaacgaagt tttgggtttg gcggcacatc tggcaaaatt aaattatcta caattgcttg
      241 aatataaaaa ccggacccgc cgacaataat gggggttcgg ccacgcttaa ttatttctgt
      301 aatctttttg ttggccagtt tttgatatct tgcgacagaa aattttcttt tgggactggc
      361 gacgtctagc aggtgatgcg gaacactccc catttctttt ttggtgactt tgccggttcc
      421 caaatcaagc cctttataaa cttggcgaga gtcagccgaa atgacttccc cgccgatttt
      481 ttgggcaacc ttgatagata gagccgtttt gccggaggcg gtaggtccta gaatgacaac
      541 gatttttggt ttttccgaca tttttaatct ttatccaatt ctaaaacatc tataaatttt
      601 tcagattttt ttgatagctt gatattcaaa gcagagctcg ttaaataggc tacctattgt
      661 tggctagcgt actttgtgcc gaaggaggga cttgaaccct cacaccttgc ggtacacgat
      721 tttgagtcgt gcgcgtctgc caattccgcc acttcggcct cattgttttt ggcctctaat
      781 agtttacctt atttttccta aaaatcaatt tatgttaata tagtgatatg ccgggaaact
      841 acgaagacaa aattttttta atagaggtag agaaaatcaa gccaaatcct ttccagccaa
      901 gaagcgaatt taataatgaa cgactacggg atttagctga ttctattaga caatatggcg
      961 ttctccagcc cttagtcgta acacgcaacg aagtagagcg cgaagacggc agtctttatt
     1021 cagagtatga gttgattgcc ggtgaaagac gttggcgcgc ctctcaattg gctggtttaa
     1081 agcaagtgcc ggcggttatc agaactggcg cgcaatccga caaagagaaa ctggagatgg
     1141 cgatcattga aaaccttcag cgcgaggatt tgaatccgat tgaacgagcg caggcttttt
     1201 tccgtttgat taaagaattc aacttaaccc acgctgaggt tgccaaacgc attggtcgta
     1261 gccgggagta tgtttcaaac accgttcgta ttttaaactt gcctcaagaa atgttggacg
     1321 ccttggtttc cggcaagatt aacgaaggcc acaccaggcc gctcctaatg ttgtctgacc
     1381 ggccccacga gcaggtgaca atttttaaag aaattatctt caaacgttta aacgttcgag
     1441 ataccgaaaa tatcgctcgt cgaattgctt ttgacaaagt tcggaaaatt gagactttgg
     1501 tagagccgga aattttggaa cttgaagaac aattgacaga aaaatatggc aatcgagtga
     1561 cggtggagaa aaaagaaaaa ggcggacgtg tgatgataga ctgggcctcc gaagatgatt
     1621 tgaagcggat tctgaaaatg atgtgcgaag tcaaaccgca atcaatcttg gaggtcaaag
     1681 acgagatggg cagtcaagca gaagtgtcag aaaaagagga acaagagaca gaaaaccaag
     1741 ccgctgagct tacggcaagc gaaaaagagt tgccaacgga agggccaaaa gacgacgaag
     1801 aaaatcttta ttttattaaa aatttttctg tttagctcag actgttaaat gccacctcgc
     1861 ccttttggcg tttgaggtgg agataggcgc gaatgtgcct tttagcgtca gtggttttaa
     1921 caaagttgag ccatttagcg cgcggtccgc tgtttttgtt ggttaaaatt tctaccaaat
     1981 cgccgttggc cagcggagcg tctaggtgac tcatcttatc attgatttta gcgccagaag
     2041 cgtggttgcc aatgtctgag tggatggcgt aggcaaaatc aatcggcgtg gcgctttccg
     2101 gcaattctac cacttcacct ttgggggtaa agacaaaaat gcggtcatga aaaaagttcc
     2161 ggcgtaattc ttcaacgaat ttttcctttt cgccatcttt tatttctttt tgccagttga
     2221 ttaggtcttg aatccactgg atatttttgg aggccttggc ggttttgttc tttttgcttt
     2281 tttcgtcgta aacaacgtgg gaggcgatgc catactcggc ttcatgttgc atttgtttag
     2341 tgcggatttg aatttccacg atagtgccgt caccaataaa gacggccgtg tggatgcttt
     2401 ggtaaccatt tggttttggg ttggcaatat aatcttttag gcgtccggta attggtcgcc
     2461 aaagactatg aacaatccct aagacctggt aacattcttc agttgttctt acaactacac
     2521 gcaaagcgga gatgtcatag acgtcgttaa tgtccatgtt tttccgaaga agtttggtgt
     2581 ataaactgta aagatattta aggcgataat caatttcaaa gtccttaagc cctttttcgg
     2641 taatggcttt ttgcaaaacg cggtaaactt tttggagacg cttaacggtt tctttgccct
     2701 tggtttttcg caggtctacg accttttggt agtcttctgg ataagcaaat ggaaaactgg
     2761 catcttccaa acggcctttc attttccaaa cgcccagccg attggctagc ggagcgtaaa
     2821 gctctatggt ttccaaagca attcgttttt gtttttcttt tggcacgtat tgcagcgtgc
     2881 gaacgttgtg caggcggtcg gccaagcgaa tcataatcac tctaatatct tcagccatgg
     2941 caacaaataa tttgcgtaaa ctttcgctgt gacgtttcgc gccctgatat ttcagcttgc
     3001 ccagcttagt gaccccttca accaaaaagg cgacagccgg cccaaactcc ttttctagtt
     3061 ctttggcgct aatatccgag ttttcaattg tgtcatgcaa gaggccggcg gcgatagttt
     3121 cagcatcagt ttcaaactcg gccagcagga gagcaacatt aaatgggtga acgatataat
     3181 cttcgccgga atagcgcttt tggcccttat gggctttttg cgcaaaggcg aaagctctcg
     3241 tgattaagtc tcgatcagag ggtgacagat tttttatgag tttaaaaatc tcctctatat
     3301 ccataagaag attctagcac gggtttgatt ttaggcaaaa attgaatagt tgtttttgcg
     3361 gttggttgtt tatttctttt tatccggtct gtgattagag caggctttgt tgctgcatcg
     3421 atcgggtata gtttttgttc cttgcatcat tagcgaacca cagtcggggc agattccgcc
     3481 agttggtttg gatttaatga tgtttttgca tttagggtaa ttggaacagc catagaaggt
     3541 tccaaaacga ccgcgttttt ctaccatggt gccctttttg caaaccgggc atttcacacc
     3601 ggtatcgccg ggtcggtctt cgccttcaat aactgcatct tttttgatat agcgacattt
     3661 ggggtaattg gaacaggcga caaaacggcc aaaccggcca tcgcgttcca ccaagggact
     3721 ttcacactga ggacattttt caccggtttc ttttggcccg gctagctctt tgccttccag
     3781 agttaaagcg ccggtgcagt tcgggaattg ggcgcaactg taaaatttgc cgttcttggc
     3841 tagtttgata atcattgggc cgccacaaac ggggcacttg gcgttgtcgg gcgctggacc
     3901 gaggtttgtg attttttcaa tctttttttt gttggcgatt tctttggtaa acggaccata
     3961 aaagtttttc aacgttttga catattctct tttgccgtcg gcaatatcgt ctagctcatt
     4021 ttccattccg gcagtgaaag aatcgctgat gtagttgtta aaatatttcg tcaaaaaatc
     4081 actgaccact tcgccggtat cagtggggac aagggactta ttttctttga tggcataacc
     4141 tcgatcttga atggttttga taatggcggc gtaggtactt ggtcggccaa ttcctctttt
     4201 ttccagttct ttgaccaaac cggcttcggt ataacgatgc ggaggttcgg tctctttgcc
     4261 gacagcttct atttctttta agaccaaagt ttccttttcg gtgagcttag gcaactccac
     4321 gtcttctcca cgagcggctg ggtcggcttt tagccagccg tcaaacagca cttgagagcc
     4381 attggtttca aaaggcggca ctttttggtc atcagcttca gctgaaactt tagtgcgtaa
     4441 aattttggcg tcagccattt ggctggctat cgctcgttgc caaattaatt gatataattt
     4501 tttttcctgc ggattgccgc cggctgattt tagactggtg tgagtggggc gaattgcctc
     4561 atgcgcttct tgagcatttt tactttttgt cttgtaggtt cttggtgagg cgtagtttga
     4621 accaaactct ttgtttatgg tcaagataat ttcactgacc gccgttttgc ttaaattggt
     4681 gctatcggtg cgcatatagg taatgtgacc agcctcgtaa agtttttggg ctagccgcat
     4741 ggtaacgctt ggcgcgtagc cgaagcgtga agaggccgct tgttgtaggg tagaagtggt
     4801 aaatggcgcg cgcggggagc gtttagcctc ggtttctgta acattggcga tttgccaaac
     4861 ggcctcgcgg gcggcggtaa caatttcttc ggctcgctcg gctgttttag gttcctcgcg
     4921 acattcaaaa ggcaggactt gtccgttttt ggtttgaaaa ttggcgctta tcacccaaaa
     4981 attttccggc acaaaagcgc gaatttcttt ttcgcgttca accaagatgc gcagggcggg
     5041 tgattggact cgtccggctg ataaaccgta acgtactttt tgccagatta aagccgacaa
     5101 gtcataacca aataagcgat ctagaacgcg acgtgcctct tgcgctaacc gcaaattttc
     5161 gtcaatggcg cgggggtgtt tgattgcttc cttgactgcc tgctccgtaa tttcgtggaa
     5221 aacaattcgc ttgggatttt ttagaccaat cgcttctttg acgtgccaag cgattgcttc
     5281 gccttcgcgg tcggggtcgg tcgccagaac aatctcgtca gcttttttgg ccagctgttt
     5341 taaatcggaa acgacttttt ctttgccggg cgagattaca taattgggag caaaaccgtt
     5401 ttctatatca atagcgtttt tgtttgactt aggcaggtcg cgaatgtggc caacactcgc
     5461 cttgacggtg taggcgttat ccaaatattt actgattgtt ttagctttgg ccggcgactc
     5521 aacgattaat aatttcatac aaacaaattt ttaaaaaaat taaaattatt taacagttta
     5581 gcaagtatta tataaattaa gtaataagtc aaaaatcgaa agttgagagc tgcataaaat
     5641 tttttagttt tttaggtgta tttctcccat ggcttctttg atcaggcctc ttatctccat
     5701 catcgacaat gtaatattgg cttgactcgg tttcaagccc gacttggcaa tcaaggcctc
     5761 cttatccaaa gattcaattt gtaataactc aaggattatt ttttcgtcag gtgataagtc
     5821 tgaaaaatta aggtcgacct tgttatcctc ttgtgtttgt aaccccaggg ccaccaaaac
     5881 atcttcggcg gaggtgaccg ggatagcccc ttgtttaatc agccagttgg ggccatggga
     5941 atttggtgaa aaaattgaac ccggaaccgc taaaacatcc cggttatatt cggtggctag
     6001 tctggcggtg attagagtgc cggatttggc gccggcctct ataattaaag ttgcttttga
     6061 caagccggcc atcaggcgat ttctttgggg aaaggtataa agcgtggctg cttgatttgg
     6121 ttcgtattca gacaagagtc cgccgcccgc tgccacaatt ttttcggcca agcggcgatt
     6181 ggctgagggg tagagaacct tctggtctaa accggagccg ggaagagcca aagtttttaa
     6241 gccgacctgc agcgctgctt ggtgggccaa agaatctatc cccaaggcta ggccggagac
     6301 aatggtaatc ggctgtcctc ccagaccgaa aataattttt tccacaacct cgcggccata
     6361 agaggaatat ttgcgggagc caaccacgca gagcagatat tcttttttac agtcggcaag
     6421 ttctccgacc aaataaagag acttcggtgg ttgagggatt tcttgtaacg ggcccgggat
     6481 ttcttgtcgg gggatttttt tgatgagcca atccattggt ctttatttta acagattttt
     6541 gtaaaattat ttaaagtctt tgttttttgg actgtgattt gctaatatta tcaatatgtt
     6601 ggacataaag tttatccgtg aaaataaagc cataatcaaa gaggccgcgc gcaaaaagct
     6661 cttggatttt gacgtggagg ctttattgga agcggataac cagagactaa agttagtagg
     6721 tgaggttgag aagttgcgcg cgcgtcaaaa tgaggcaagt cacaggattg ccaaagttaa
     6781 agacgaggct gagcgagatg agttgattga gcaaatgaaa gatgttaaaa ctttgcgcga
     6841 gggaaaagag gaagaactga agcaagtgct ggagacctgg aagagtttat tgctaaaagt
     6901 gccaaacatt cccgacattt cagtgccgga aggcaaagat gatagtgaaa accagcagat
     6961 taaaatttgg ggggaccgcc ccaagtttga gtttgccccc agaggtcacg ttgaattgat
     7021 gaccaattta aaattggctg attttgaacg gggggtcaag gttgccggtt ttcgtggcta
     7081 ctttttaaaa aatgaagcag ttttgttatc aatggcggct tggcaacatg ctatgaaaat
     7141 tgctctagag ggcggttttg ttcctctttt gacacctgtc ttggatcgac cggaaaactt
     7201 catggggaca ggatatttgc ctgacggcga agaagattta tataaaaccc aagacggtga
     7261 ttatttggcc ggcacggccg aggtcccgac gatgggctat cattttgatg aaattgtgcc
     7321 ggcggatcgt ttgccgtata aatttatcgc tttttctcct tgttaccgtc gggaagcggg
     7381 tagccacggt aaggatactc aaggtttaat tcgtgttcat gagttttata aggtagaaca
     7441 agtggtgtta tgcgaagcaa gccacgaaga gagtgtcaat tggcatgaaa aattgttggc
     7501 caacgccgag cgttttatgc aagacttgaa aattccatat cgggtggtag ttaattgcac
     7561 cggtgatttg ggccaaggcc aggtcaaaaa atatgatata gaagcttggg tgccatcgga
     7621 agggaagttc cgcgagactc attctatttc ttattttcat gatttccagg cgcgacgttt
     7681 gaacactcgt taccgagaca gtgaaggcaa acttcgcttt gttcattctt tgaacggcac
     7741 cttagtggct tcgccgcgaa ttctcgtttc gctcttagaa aattatcaac aagccgatgg
     7801 ttccgttgtc gtgccggagg ttttgcgacc atatcttggc aaagatgtca taaaataaaa
     7861 agtcatggcg gcgcccaaaa gcaagcgtga cattttaaga aggtcaaagg aacgcgaaca
     7921 aaaaatgcgc aagttccggc ttggtggagg ttttttgttg gtggtcatta ttgtcggtgg
     7981 ttttttggga cttttgaatt ggaatttttt ctgtttcaac accgtgattg tcgcgggaga
     8041 gacaaacgaa aacaaagaag caatccaaac ttatgttttg gaaagtatcg ccggtcgtta
     8101 tttgggagta gtgcccaagg atagcgtctt ttttctgcgt aaaaagattt tggctgaagc
     8161 ggttaaacat catttcccca gattgaaaga tgttcaggtc agcttgccgg aactcaacac
     8221 tttaaaaatt tcagttgagg acaaggaggc gaaacttgtt tggtgcgatg gccaagatgg
     8281 taaaaaaagc ggtcattgtt attacctcaa cgaggacggt gaagtttact ccgaagcccc
     8341 caatttttcg gaagctgtta tgacggaaat catcgctcct ttgccggaat tgccgattgg
     8401 taaaattgtc cttgcttcaa gcaccgtcga gaaaatccaa gttttggcca gtgccggagg
     8461 acgtttatta aatgggttgc ccgggggcta cgcgcctgac cttgtgtctt ggcagccatt
     8521 gacggccggt gattgggcgg ggcgagtcgc ggaccgagaa ggccgggtct ggcgaatctt
     8581 gttcaacgaa aaaagcaagg ccgctgattt ggttggcggt ttgggttcta tcctaaaaaa
     8641 cgaaactttt cttactgact ggtccagaca aaagggccgt cttgaatata tcgacctaag
     8701 gttcccgcaa aagatattct atcgttttat ccagtagtca taagccgaaa atgtttgtat
     8761 aataagggaa catgaacatc acccttgtca taagtcatta tttcttttgg cactattcag
     8821 ccgctctggt taggtttacc gccatttaca aagacatcat attttttgtg gcagacttct
     8881 tttctttgcc gatattattg aaatcttttt ttgcgccatg gcgtcgtttg gccgagcctt
     8941 acccggaaga taaacttgat ttttcagaaa tgatgtcagt gtttatagtc aatagtctgg
     9001 tgagattggt gggggttgtg atgcgttcta ttatgatttt ggttggttta atcgcgcttg
     9061 ttcttaccgt cgtcctttat ccagtcttgg ttttgttatg gttggcctta ccgctagtga
     9121 tagttgtttt attagcagtt ggtttgaagc ttttgttttt ctagatcatg gcgaataaac
     9181 tacaaacata tcttagggcc agccgccttt acccggttct ggttttggat tcgttaattg
     9241 ccaaacgaaa gcgcgattgg ctgataaata taaccggcac cttgattttg ctcttgctct
     9301 tgccgattct aagttatcag atttttagca gcttgctctc ttcttctatt tttgatattt
     9361 ggtcgctttt ggtcccccgc ttttggggcg ctatttttgt ctgttcagcg ctttattttt
     9421 tggctaaatc gttaaccttt tattttaatg attcctatta ctttgacaat gtggccctta
     9481 acaagtatgc caatgaagat ttattcagta ttaccgtcgg ccgagttctc tatcaggctg
     9541 acaagggaga tgctctgcgc ggttttttta ggtcgcaaac cggacaagaa attttgtggc
     9601 ggctaggagt agatgaggaa gaacaaaaaa agtttttagc aacgcgtcag acagtgagtg
     9661 atctccctga acagccgttg aatggtgttc taaagatgcg cgcgctgact caatttttat
     9721 atgacaaaaa tcctgacttc gcgcttttcc taaaaaaatt tggggtgggc gaaaaagatc
     9781 tcttgggggc gaccgattgg gtggtcaacc agattgaaac aaacgagttt gtcaaacgat
     9841 ggtggtcaaa ggaaaatcta aaacgaattc ccggtttggc caaagactgg ggctttggca
     9901 gcacgtttac tttggacaaa tatggttggg atatgcttcg gggcgcgcgt tactctaact
     9961 cgagctatga atattctttg tttgccgcgg aggtttccca attggaaaat attttagcta
    10021 aagaaaaaga ggctaacgcg attttagttg gggattcacg agaagaagcg ttggacgttg
    10081 tttggcatct catcaggaag attcaaaatc gaacggcagc gtcagctttg gaacacaaac
    10141 gcccaatctt attcaacgcc tcggtttttc tctctcattt caaagaacga caaggagttg
    10201 aaaaagaatt gcttaaaatt ttagacgaag cgcggaaagc gggcaatatt atcttggtca
    10261 ttgatgacat ttacaactta atgaccggtt ttcaaaatct cggctcatct tttttggggt
    10321 tggctgagga gtatttgcaa aatggcgctc ttcaaattat cgctgtgaca acaactgacg
    10381 cctaccaccg ttttttggcc ggcaactccg ggttgatgaa tgcttttgac cgcattttcg
    10441 taaaaaccat gagctccgaa tcattggttc gcggtttgca agaaacggtt tggcaagtgg
    10501 aggccaagca taaattgttt ttcacctatc aatctttgca tgaaataatc ttgagcgcgg
    10561 acagctactt cagcgaaagc gcgtcaagcg acaagtcggt ggacttaatg actgaaatgg
    10621 tttcttgggc ccaaaaccag ggcctgaaga ttatccgtcg aggtgatgtg cagaagttca
    10681 tttccagcaa gaccaatgtg ccacttggcg aaattgggcc tgaggaacga acaaaattgc
    10741 aaaatttgga ggttgaaatc cataagcggg taatcggtca agacgaagcg attaaatctg
    10801 tttcaggggc tttacgtcgg tcgcgcgccg gtgtccgtaa tcttaaccgg ccgattggtt
    10861 ccttcttgtt cttgggcccg actggtgtcg gcaaaacaga aaccgccaaa gccttggcgg
    10921 aagttgtctt cggacaggaa tccagtttgt tgcggttaga tatgtcagaa ttccagaacg
    10981 aagatgcttt gactagattg attggctcgt ttgaaacagg taagaccggg attttggtgg
    11041 atatgttacg tgaacaccct tatgctgttt tgctgttaga tgaatttgaa aaaaccaacc
    11101 gcgaagtttt aaatcttttc ttgcaaattt tggatgaggg actcttttct gatatgagtg
    11161 gcaagcgagt taacgccaga aatattattt ttgttgttac ctctaacgct ggcgcggaat
    11221 taatctggga aaatttccga caaggcaaaa aagaagaaaa tactagtgat aacttgataa
    11281 accatatcgt ggccaagggg atttttcgac cggaactcct aaaccgtttt gacgcgaccg
    11341 tcgtttttga accgctctcg ccggagagtt tgagagatat tgccagattg atgctcaaga
    11401 aacttggcaa gcgtttggcc ggccaagggt tggaactggt ggtcagcgat tatttgacgg
    11461 aaaccgtggc gaccctgggc gccaacgaag tttttggcgc tcgtccgatg cagcgtttta
    11521 ttcaggataa tatagaacaa aaagttgccg acgcgttgat tgatggtcgt ttggttcagg
    11581 gtgacaaggt cgtatttcag gaacctaact tagaactgac gaatgcctaa aatggaacga
    11641 agcggcggaa agtcggggaa attttcattt caggtggtta tcgcgctggc gataatttta
    11701 attttgggct tcattctttt tcgggtcagt caatttttgt atgccggtgt ttttggcaat
    11761 gaggcggtta gttatcaagc cggggcggct tccgccggct ccgttttaaa gagtgattac
    11821 accgatttta aagttggcgc gacacctgtt cccaagcttg gcaaatcggt tattattgac
    11881 gtcaagaatc aagaactcca cctttttgaa aaaggagaat tggtaaaatc tttttcgttg
    11941 ctagctatcc ctgaaagcgg ctcggcctgg gaaacttcag ggggcggttt ttccgtaata
    12001 aaaaaagaag aaaatcactt gtcgccgatt accggcttgg agttccccta cactgttcaa
    12061 ttttttggga actctcttat tcatggtgag ccgatggata taaaaggacg aattgcaaaa
    12121 acagcggccg gcggctttcg attaaaaaat gacgacgcgt tgactgtttt cgactgggtt
    12181 gattttgaca ccaaagtttt ggtttacaac gcgcgctgga ctatgggtgg aaacaatcat
    12241 ttttcataca ttatcaaaaa atctcagccg gctcctctga ccgccaaatc ttacctagtt
    12301 tctgatttaa agaccaagga ggtgattttg agcaaaaaca aggacaaggc ggtgcccatt
    12361 gcttcggtga ccaagttaat gaccgctttg gtggcgctag aaagacttga gccgaaaacc
    12421 gagatagagg tttctcgtca agcttacgaa acttatggcg acagcggcta ttttcgaacg
    12481 ggggacaagc ttcctttgga aattatgctt tatcctttgc tcttggaatc aagtaatgac
    12541 gcggccgaag cgattgccga aaaggttggc cgggaaaaat ttattgttga aatgaataaa
    12601 aaagccaaag agctcgggat ggaggaaacg ttttttgaag accccagtgg tttgtcacct
    12661 aataatgttt cttcggccgg ggatttggtt agattggctg attatattca tcagaataaa
    12721 atctttttgt ttgatataac aaaattaaag gagtatcagc atggacgctt cgtttggaca
    12781 aatcgcaata atttaattgg gatgaattat tatgctggtg gcaaaaacgg ttatacggat
    12841 gaagccaacc gaactttgct cgcgctgtat gacttacctt tgtctgagtt tgagacgcgg
    12901 aaaatagttt tggttttgtt ggggagcgat gaccgcaagt ccgacaccag aaacattgta
    12961 aattatatcg ccggcaatgt ctcttatagc gggccatctg tttttaaaac tttggatgat
    13021 tttgggtggg atgaaatata aacagcaaat atgaatttag ccaaaaaatt actgccgttg
    13081 ttgggcggtc tggttgtcgg tctgagttta attttctggt tcttttgttt tgtttcaagg
    13141 caagatgttt tttttaacga agaagtaaaa ctaaacccgt cttttaagtc tgaggttgaa
    13201 acgggagagg taactttatt ggcagttggc gacattatgc ttgaccgagg cgtggcccag
    13261 ctggtcaaca aaaaaggcgg cggcgacttt gcttatgtct ttgaaaacat tcgcgcgtta
    13321 aaaggcgaag ccgacatttt attgggaaat ctggaaggac cggtttctga ccgaggagcg
    13381 gatgttggca atctgtattc ttttcggatg tcgccaaaag ttttgccagt tctgaaagga
    13441 gtcggatttg atatcttgaa tgtggccaac aaccatgccg gcgattgggg cgtggccgcc
    13501 ttccttgact cgctggagcg tctttctgcc gccggcttgg cctacattgg cggtggttta
    13561 accaaagttc aagcggaaaa gccggttatt attgaaaaaa acggattaaa agttggtttt
    13621 ttgggttttt ccgatgtggg gccaaactgg ttggcagcca ccgaacgaga ggccggcatt
    13681 ttgttggccg gtgatatcca tttcagcgaa ataatcactc aagcggctgg tcaaacagat
    13741 gttctaattg tcatgattca ttttggtgaa gagtaccagg cgttccacaa tgagcggcaa
    13801 gaaaacttag ccaaaacggc gcttcaagca ggcgcgcggg cggtggttgg tcaccatccg
    13861 cacgtggttc aagacgaaga gaacttgggc caccaatata ttgcttacag tttgggcaac
    13921 tttatttttg accagaattt ttccgttgaa acgatgtccg ggcttgcctt aaagctaaag
    13981 ttgagtcaag atggcgtcgc cggcgtggaa aaattgccta tttccataaa tcataatttt
    14041 cagccaaatt ttgcgaatta aaatgaaatc tttttgggga aaattgaata aaccgttttt
    14101 tgctttggcg ccgatgtatg atgtgacgga tgctcctttt cgagcgatgt ttgccaaata
    14161 cagcaaaaaa gccaagacca aagaagcgcc ggacctggtg ttgtttaccg aattcgtgtc
    14221 ttgtgatggt ttggcaagcg aggagggccg gtccaaattg ttgcgggaac tttattatac
    14281 cgaagctgaa cggccgattg tggcgcaagt ttttggcgcg cggccgggga acatggaaaa
    14341 agccgctaga ctgattagcg agcttgggtt tgacggctta gacttgaata tgggttgtcc
    14401 cgataaagca attgaaaagc aaggcgctgg cgccgctttg cttaaagacc caatcttggc
    14461 gcgagaggtt gtcgcggctg ccaaacaggg ggcaggtgat ttgccggttt cggttaaaat
    14521 ccgtttgggc tataacaaat tagatttagt ttggttagag aaaattattg caaccaaacc
    14581 ggcagccctg gcggttcact tgaggaccag aaaagagatg tccctcgcgc cggcgcggtg
    14641 gtctgatttt ccgccgattg tcacgttggc tcacgaggcc ggtttggtgg tagttggcaa
    14701 cggcgacgtt gttgagatag cagatgggca acaaaaagcg aacgcgagtg gcgctgacgg
    14761 cattatgatt ggccgcggcg cttttgggcg gccgtggctg ttttctggtg gggaagagcc
    14821 cgcgcttaaa gaaaaattaa aaatattgca agagcaaacg gaactttttt gggatttgta
    14881 tggtccgacc gaaaccaaca agaaactttt tgccgggcac caaaaaaact tcgcggtgat
    14941 gaaaaaacat ttcaaggctt acgtcagcgg ctttgccggc gcgagcaatt tgcgcgccaa
    15001 actgatggcg gcggaaaatc cggaggcggt caaaaagata atcagagaag ccgaaaaaga
    15061 gggtttaggt tgcctagttt agagtaaggg ctgtacttgc attttttgct aaaatatgct
    15121 ataattatag ataggaaggg tggttcaatc ataatcccca aagataaccc ccaaaacaaa
    15181 tacttaaaca atcgctcatg cctgtcaggg tggggcgatt ttctttttct tcggtctttc
    15241 ggtataatct caata
//
LOCUS       DAOOLO010000013        14727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_4355_length_14727_cov_5.904444, whole genome shotgun sequence.
ACCESSION   DAOOLO010000013 DAOOLO010000000
VERSION     DAOOLO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_14727_cov_5.904444"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLH96_01400"
     CDS             complement(<1..190)
                     /locus_tag="PLH96_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60459.1"
                     /translation="MESYINIIKVFAPSTVAFFFGMAFTPFLTDFLYKHKLWKKKAGK
                     ITPDGKETPIFNSLHKEEE"
     gene            complement(196..1188)
                     /locus_tag="PLH96_01405"
     CDS             complement(196..1188)
                     /locus_tag="PLH96_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPB60460.1"
                     /translation="MILKNKKKMEPTETTTKNKEVILKKDRAVWWLLLSFFLISASVF
                     SILWVWPKTEKASAEKIVQTPTTTPKAVFFKDLEVEAKSFVIYDPQTKQVIYSQDAET
                     ARPLASLTKVMTALAATELVSPDDTTVVIDENALAEDGDVGLHPYSLWKLKNLLNLTL
                     VSSSNDGAKAIANAVSAISTKDFVAEMNRQAMEIGLSSANFFNATGLDLPSGGAGAYG
                     SPKDVAKLFAYVLKNKPELLAATKESHLKETSMENEVYYASNTNSLASEIPGLLASKT
                     GYTQAAGGNLAVVANLGLNRPIVFVVMGSSQTGRFHDMEKLITKTQAYLANASH"
     gene            complement(1133..1693)
                     /gene="scpB"
                     /locus_tag="PLH96_01410"
     CDS             complement(1133..1693)
                     /gene="scpB"
                     /locus_tag="PLH96_01410"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB60461.1"
                     /translation="MDLDAKIEALLFHKTEPLTLDKIAEIVGKNEEETEEALAGLAQK
                     LIGRGIVLLKKDNSYLLGTSPEASGLIEKITKDELSADLGRASLETLTIIAYEGPLSR
                     AEIDYIRGVNSSFILRNLMVRGLIERAPDKNDSRRFLYRPTFELLRYLGVSEASELPE
                     FESIREKINDFKKQEENGTDRNHNEE"
     assembly_gap    1729..1828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1829..2250)
                     /locus_tag="PLH96_01415"
     CDS             complement(<1829..2250)
                     /locus_tag="PLH96_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB60462.1"
                     /translation="MDAETTNFEVKIGEFEGPLDLLLSLIEKRKMHISDVSLAQVADD
                     YISYLRSFDNLPMDNTANFILIASTLMLIKSLSLLPGLAVTEEEKESIEDLENRLKHY
                     QRIKELSLHIKARFGQNVIFGREPSRESVIVFSPTDEI"
     gene            complement(2637..3968)
                     /gene="aspS"
                     /locus_tag="PLH96_01420"
     CDS             complement(2637..3968)
                     /gene="aspS"
                     /locus_tag="PLH96_01420"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HPB60463.1"
                     /translation="MIDRTKIGELKEKVGQEVTLAGWVDIRRDHGKLIFIDLRDASGK
                     VQMVVLPNHEEAHQTAQEIRPEWVIKVKGLVNKRPEKMIKADQQNGDIEIEVLEIEVL
                     GPAKEMPFDPDTELNFDTYLDYLPLTLRGDRPKAIFKVQANIIRAFREFFNQEGFVEI
                     QAPKLIGDDAEGGANSFDIEYFGHTAHLAQSPQFYKQIMVGIFERVFATGNVYRAEKH
                     STTRHLNEYTSIDAEMGFITDHTDIIKIETKLLKFIILELEKRCQEEFKFLKAEIPKI
                     PDEIPSLKLREAQEIIKKETGEDYTQEPDLEPQNERWLCEYAKKEYGSDFIFITHFPM
                     SKRPMYTYEDENDPGYAKGFDLLFRGVEITTGGQRIHDYDKLVEAIKAKGLDPEKFTY
                     YLQAFKYGMPPHGGFGMGLERLTAKLLGLENVKEATLFPRDLNRIDTLLSK"
     gene            complement(4032..5012)
                     /gene="trpS"
                     /locus_tag="PLH96_01425"
     CDS             complement(4032..5012)
                     /gene="trpS"
                     /locus_tag="PLH96_01425"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB60464.1"
                     /translation="MPENNKKVLLSGVKPTGRPHIGNYFGAMKQFVDLQADYKSYIFI
                     ADLHALTTARDPKQLRADTLDLAIDYLAIGLDPEKVVFFKQSDVAEHTELAWIFNCLT
                     TMPFLARAHAFKDAETKNKEVNVGLFDYPLLMAADILLYDTDVVPVGQDQKQHVEITR
                     DTAEKFNFTYGETFKLPEPLIMDEVKTLPGLDGQKMSKSYSNTIPLFADEKEIKRLVM
                     TIKTDSKDVSEPKNPEECNVFALHKLFSGEQLSELKKRYVEGGIGYKESKEILAENMN
                     KYLAPMREKRAEIAKDPDQVLQILKNGANSAREQAKLKMKEVRERVGIDL"
     gene            5185..6690
                     /locus_tag="PLH96_01430"
     CDS             5185..6690
                     /locus_tag="PLH96_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB60465.1"
                     /translation="MQKNSNKKIAGQILAVKNISLSFGPHPVLDEVSFEVQAGDKIGL
                     IGPNGSGKSSLMKLLVGQLESDKGEIVLSPNVSVGFQPQEIMEDMTALEYLEESLNSQ
                     ENNSSHYALGLNDEILAKKFSEMSGGERSKIVLAKIMSLKDDLVLLDEPTNNLDYSAL
                     QRLEKYIKDSRSTFIIISHDRKLLTDLTNKIFEIDSDKRSLSVFIGNFEEYLKEKKRS
                     KIRQYEKWQDFRDEEKRLTVSARNKYEWAEKGQKPKITDRYRGNIGLQKDRSAQVFHR
                     LAKNLERRLENLEKVEKPKGKLPQKFYFNINRKGEGRILKIESLEKKLPNGKTVGPLS
                     FEVNLGERLLISGPNGSGKTTLMKTIKGELKPDSGKITFAEGLKISYLPQDLTALPGQ
                     TTLDLFFAQVELPQTEGRNYLVRFGLKEEDVKKKVGNLSPGQRSRLFLALVMAQRAEC
                     LFLDEPTNHLDLEGLEALEKALLGYRGGLIAISHDRQFVQKIGFKQEIKLG"
     gene            complement(6708..7364)
                     /locus_tag="PLH96_01435"
     CDS             complement(6708..7364)
                     /locus_tag="PLH96_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60466.1"
                     /translation="MFILLGTITGFLYGSSRRSANQAVKSLFAIFLFFSCYALILAVF
                     SIIGQNKPYTLILGYNFAVLFAFLALYFITEIPTFSENQLIKKYSRYISNFIILIGII
                     VIAIQLIDMRLPITGENGVILWNQNPVAAWLTGLTILGYMLAWAYFTCQNMKVIKNKQ
                     QRLKLYALGADAISVGFSALIYFPSQNEWQSLASFLLIIPAYIFTIYSLAQSRKAKAA
                     "
     gene            complement(7468..7794)
                     /locus_tag="PLH96_01440"
     CDS             complement(7468..7794)
                     /locus_tag="PLH96_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60467.1"
                     /translation="MPDGESVSVAELIKLKKTFRKEVGRSELDRVVREAQLTAAKICL
                     EIELPPYKPKAIKPSPELARWLSAREVLGKREERKTKQRKERLQSRITLRRRYPVPAN
                     TYVFCK"
     gene            8164..9390
                     /locus_tag="PLH96_01445"
     CDS             8164..9390
                     /locus_tag="PLH96_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60468.1"
                     /translation="MIKEMTKNSSWEHSFYKQAFLWMYRARNFPSRVILPVNSSGKFQ
                     LEVRDFGTNYHRQITKSSQGSIFGSISPSGKFVFYLNDKGGNEIGHFVREPFRGGKKV
                     DITPNLPSYFAYEVSSDYNDERLCFYASIKEKNYVYLVETRQGKEGFKSKLLYETEDL
                     ILGTVISPDGKQTCVSISLGGKKNKSQVLVFDNNNGKLICKINFLAGDFIAQAFSVDS
                     DKPLILGILNKDGFYRPVWFDFLKKQITEIKNPKFKGNVFVLEWLEDQNKILFGREYK
                     LKHSLLLYDLNKKTILPIGPDYGSLDLFFGSVTSLLDGSLILRWQSFDIPPQIIKIFD
                     HNYQKTKSLYLAKRQIRKKKLLKVWFSSLLMAHRSKCGWLGQQKSKNFILLLSTSTEV
                     HMVLSMILFLPRLRFY"
     gene            9279..9995
                     /locus_tag="PLH96_01450"
     CDS             9279..9995
                     /locus_tag="PLH96_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPB60469.1"
                     /translation="MAWPTKKQKLYPFVIDIHGGPHGSVNDSFSPEAQILLDEGFGYC
                     AVNYRGSTGFGKDFERAIYGNPGYFEVEDIVSARGWLISKGQADPDKIILFGWSWGGY
                     VTLLALGKYPNLWAGGIAGTPIGDYLSQYEDEPAFFKAIDRELFGGSPEQKPAAYKKS
                     SPITYVDNFKKPVLVIYGENDARCPTKQIVNFINKLKAKNKLIKVKKFSSGHTGEFSN
                     TKIRISNFKEILKFAKRLTR"
     gene            10656..11363
                     /locus_tag="PLH96_01455"
     CDS             10656..11363
                     /locus_tag="PLH96_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60470.1"
                     /translation="MKKLRFCTNKQLDEIMIEQFLHEQGGGIDFGKGVIKLHPRLKLA
                     KSLENVEQKKKIIHLYIHKYYHSHKEEINYSIKNLQKAWHKKEKEFVIETEKFFSNFR
                     FPEGKYIAYASIVDCNPRFLDSKTFQFFYKKKLADAIHTIVHELLHFIFFDFVEKKLK
                     SEIKILTEEQLWDLSEIFNVVVLGSAQYQNIVDKKYVFPYPHHKPLLSQFRKAYKDSC
                     NAEEFIKRGVTIIKTKK"
     gene            complement(11409..12776)
                     /locus_tag="PLH96_01460"
     CDS             complement(11409..12776)
                     /locus_tag="PLH96_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB60471.1"
                     /translation="MKLKQSPFLHLVPLDNGYVALYNSLNLEVAFLKEDFIDRHRHSQ
                     AWIASDVSSKHVLESLVKLGLLIKEQADGEERYREYQKALEEPSINILYLLLSDACNI
                     RCRYCYFLAPMPYDYRFSVMKKEVAIKALDLFARCVQKSIAKGHPEQHIVIYGGEPTI
                     NKGVLLDALRYIDALKEEGLLPKTISVTLNTNGILLDEEILNQCKSSGVVVAISIDGP
                     KDVHDQMRVYPSGKGTFEEVLRSYRLAQDKGVKTGICVTIDQHNLFRMEEIVHWLANE
                     LGAKGMGFNILIENSVGQAEEKHDRYAEIVAQELIKSFRVARTEGIYEDRMMRRVKNF
                     LDKEPVLSDCGGCGLQIVVSPNGEIGTCQAFCGKKEFFVTEPFETFDPESHSFWKQWR
                     KRSPFANETCKTCIALGNCGGGCPYNAHRIKGSIDALDERFCAHAIATTHFLIRDLWE
                     KQKQK"
     gene            12975..13547
                     /locus_tag="PLH96_01465"
     CDS             12975..13547
                     /locus_tag="PLH96_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60472.1"
                     /translation="MSLNKELKLKIEEMTRVDQNLRKKQWKEWKNLVRENTNKNDPKL
                     LAMVQKMAKELLRVDKKNTLEMKKIISKYGWPGRSLVGKKGALNAWLLVQHADHDVDF
                     QEKCLKLLTAAAEKGEVDKKRVAYLTDRVLLHRGKKQVYGTQLRSFPQKGKTILKPQP
                     IKNRKKIDELRKGMGLEPLKIYLDKMRKMY"
     gene            complement(13670..14644)
                     /locus_tag="PLH96_01470"
     CDS             complement(13670..14644)
                     /locus_tag="PLH96_01470"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60473.1"
                     /translation="MRSKILVVTSSEVSDVESVLNFLPREDTFVFKTDYFENYGVTQE
                     TSSGTDTFSITDDSSRIESSDISSIWYRRPGKKQQGMGDLELADKQYAEQEVSRFLRA
                     LWNSQYPHNVLWVNHPYLSWKIECNKQLQIKEACKAGLDVPNSIVTNDHERARDFLSK
                     HGACILKTFGTFSKDEEGWPMGVYTTPITLQDLEANLDELKLCPVILQSYIPKRLELR
                     VTVVGDNVFACAIHSQDSDRTKHDWRKYDFVNVKHEVFELPQYVKDRLLTCMRSWGLK
                     FGAIDMVITPDDRYVFLEVNPAGQWGWIEDLTQLPIAEAIATLLKNGR"
BASE COUNT         4118 a         2923 c         3153 g         4433 t
ORIGIN      
        1 cctcttcttc tttatggaga gaattgaata tcggtgtctc tttgccgtca ggcgttattt
       61 ttcctgcctt ctttttccaa agtttatgtt tatacaagaa gtcggtcaaa aatggcgtga
      121 aggccatgcc gaaaaagaaa gcaacggttg acggagcaaa gacttttata atattgatat
      181 agctctccat atattttaat gactggcatt tgccagataa gcttgtgttt tagttattaa
      241 tttttccata tcgtgaaaac gaccggtttg gctggaaccc atgacgacaa agacaatcgg
      301 tcggttcaaa cccaggttgg cgaccacggc caaattgccg cccgccgctt gggtgtagcc
      361 ggttttggag gcgaggaggc cgggaatttc actggcgaga gaattggtgt ttgaagcata
      421 gtagacttca ttttccatgg aggtttcttt caaatgactt tctttcgtgg ccgccaaaag
      481 ctcaggctta ttttttagca cgtaagcaaa caatttagca acgtctttgg gtgagccata
      541 agcccccgcg ccccctgaag gtaagtccaa gccggtggcg ttaaaaaaat tagcgctgga
      601 aagaccaatc tccattgctt ggcggttcat ttcggcaaca aaatctttgg ttgaaatggc
      661 cgaaaccgcg ttggcgatgg ctttggcgcc atcgttggaa gaagaaacta gggtcaaatt
      721 caaaagattt tttaatttcc agagcgagta aggatgaagg ccgacgtcac catcttccgc
      781 taaagcattt tcgtctatga ctaccgtcgt gtcgtccggc gagaccaact cagtggcggc
      841 cagagcggtc attactttgg tcaaagaggc caaaggtctg gcagtttccg catcttggct
      901 gtaaatcact tgtttggttt gcgggtcata aatgacaaat gatttggctt ctacctccaa
      961 atctttaaag aaaactgctt tgggagtggt ggtcggtgtt tgaactattt tttcggcgct
     1021 ggctttttcg gtttttggcc aaacccataa aatggaaaaa actgacgcgc tgattaagaa
     1081 aaaagacaaa agcagccacc aaacggcgcg gtcttttttc aagataacct ctttattctt
     1141 cgttgtggtt tcggtcggtt ccattttctt cttgtttttt aaaatcatta attttttcgc
     1201 gaattgactc aaactccggc agttcacttg cttcgctgac tcccaaataa cgcaacaatt
     1261 caaaagtcgg ccgataaaga aaacggcgcg agtcattttt gtcgggcgct cgctctatta
     1321 gtcccctgac catcagattt cgcaaaatga agctggagtt gacgccgcgg atatagtcaa
     1381 tttccgcccg gctgaggggc ccctcgtagg caatgatagt cagtgtttct aaactggcgc
     1441 gccccagatc ggcagacagc tcgtcttttg ttattttttc aatcagccca gaggcttccg
     1501 gcgaagtccc aagcaaataa gagttatctt ttttgagcaa aacgatgcct cggccgatga
     1561 gtttttgcgc caaaccagcc aacgcttctt ccgtttcttc ttcatttttc ccaactattt
     1621 cggcaatttt gtcgagggtt aagggttcag ttttatgaaa aagcaaagct tcaattttgg
     1681 cgtctaaatc cataatgtaa ttatattata atgcaaaatt taaaaattnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gatttcgtca gtcggtgaaa aaacgatgac
     1861 cgattcacgc gaaggttcgc gcccaaaaat aacgttttgg ccgaagcgag ctttaatatg
     1921 gagacttaat tctttgattc gttggtagtg tttgaggcga ttttctaaat cttcaatgct
     1981 ttctttttcc tcttcggtca ccgccagtcc tggtaaaagc gagagggact taatcagcat
     2041 caaggttgaa gcaattagaa taaaattggc ggtgttatcc atcggcagat tatcaaagct
     2101 ccgtagataa gaaatgtaat catccgccac ttgggccaat gagacatcgg aaatgtgcat
     2161 tttccgtttt tcaatgaggg acaaaaggag gtctaacggg ccttcaaact cccctatttt
     2221 aacttcaaag ttagttgttt cggcgtccat ttcggcctta ttttagcata tctttccctt
     2281 tctcgccaat tttctcagtg ccttctcttc ctcagaacct tctcagaatt ttcccaggct
     2341 tcttgtccta ggtccgtcct agtccaaaaa gtggactagg acggacctag gacaaatcaa
     2401 cgtgttgtct ttaaaatgac ttaaaagact taaaatggct caaaataaaa acttggtgga
     2461 gatattcctc gccaagaatc agtccctcgt cttttctttt tgccttctct aaagctttgg
     2521 cccaggttga agctttttag gagagtagct ctcctaaaga agatttagcc gttatttgcg
     2581 cgaagaccag cttcgcgcaa ataacggccc tgggaggttt tttcttttgc aaaagattat
     2641 ttagacagca gggtgtcaat tcgatttaag tcgcgcggga agagggtggc ttctttgaca
     2701 ttttccaggc caagcagttt ggcggttagg cgttccaaac ccatgccgaa ccctccgtgc
     2761 ggtggcatgc cgtacttaaa cgcttggaga taataagtaa atttttccgg gtccaagccc
     2821 ttggctttga tggcttctac cagtttgtca taatcatgga ttctttgtcc gccggtagtt
     2881 atttccacgc cacggaacaa caagtcaaac cccttggcat aacccgggtc attttcatct
     2941 tcataagtat acattgggcg tttggacatt ggaaaatgag taatgaaaat gaaatcagag
     3001 ccatattctt ttttggcgta ttcgcacaac caacgttcat tttgtggttc caggtcgggt
     3061 tcttgggtat agtcctcacc ggtttctttt ttgataattt cttgcgcttc tcgcagtttt
     3121 agagatggaa tttcatctgg aatttttggg atttccgcct ttagaaactt gaattcttct
     3181 tggcatcttt tttctaattc aagaatgata aatttaagca acttggtctc tatctttatt
     3241 atgtcggtgt ggtcggtaat gaagcccatc tcggcgtcta tgctcgtgta ctcgtttaaa
     3301 tgtctggtcg tgctgtgttt ttcggcgcga taaacgttgc cggtggcaaa cactctttca
     3361 aaaataccaa ccattatctg tttgtagaat tgcggacttt gtgccaagtg ggcggtgtgt
     3421 ccaaagtatt caatatcaaa cgagttggcg ccaccttcgg cgtcatcacc aatcagtttg
     3481 ggggcttgga tctccacaaa accttcttga ttaaagaatt cacggaaagc gcgaatgatg
     3541 ttggcttgaa ctttaaagat ggctttcggc ctgtcaccgc gtaaggttag aggtaaataa
     3601 tctaaataag tatcaaagtt tagttcggtg tcggggtcaa acggcatctc tttggccgga
     3661 ccaagaacct ctatttccag aacctctatt tcaatatcgc cgttttgctg gtcagcttta
     3721 atcatttttt ccgggcgctt attgactagg cctttaactt taatcaccca ctctggtcgg
     3781 atttcttgag ctgtttgatg agcttcttcg tgattgggca aaaccaccat ctggactttt
     3841 cctgatgcat ctctcagatc aataaagatg agtttgccat ggtcgcggcg gatatcaacc
     3901 cacccggcca aagtcacctc ttgcccgacc ttttctttta actcgcctat ctttgttcta
     3961 tcaatcattt ttttaaattt tcttgtgttt tcttatttta aattttaatc tttacatttt
     4021 acgctctctt cttacaggtc tattcccacc ctttccctca cctctttcat cttcagtttg
     4081 gcttgttcgc gcgcagaatt ggcgccattc ttgagaattt gtaaaacttg atccgggtct
     4141 ttggcaatct cggcgcgttt ctcgcgcatg ggcgcgaggt acttgttcat attttccgcc
     4201 aaaatttctt tagattcttt gtaaccaata ccgccctcca cgtaacgctt cttgagttcg
     4261 gacagttgtt caccagagaa aagtttatgc aaagcaaaga cgttgcactc ctccggattt
     4321 ttgggctcac tcacatcttt agaatcggtt ttgattgtca tcaccagtct tttgatttct
     4381 ttttcgtcag caaaaagcgg aatggtgttg ctataactct tgctcatttt ttgaccatcc
     4441 aagccgggta gagttttcac ttcatccata attaaaggct cgggtagttt gaaagtctcg
     4501 ccataagtga agttgaattt ttcggccgta tcgcgcgtaa tctcaacgtg ttgtttttga
     4561 tcttggccaa ccggcaccac atcagtgtca taaagcaaaa tatccgccgc cattagaagc
     4621 ggataatcaa acaacccgac attcacctct ttgtttttag tctcagcatc tttaaaggcg
     4681 tgagcgcgcg ccaaaaatgg catagtggtc agacagttaa aaatccaagc tagttcggta
     4741 tgttcagcaa catctgattg cttgaagaaa accacttttt ccggatcaag cccaatcgcc
     4801 agataatcaa tcgccaggtc caaggtatcg gcgcgaagtt gctttgggtc gcgggcggtg
     4861 gtcaaggcgt gcagatcagc gataaaaata taacttttgt aatcagcttg aagatccaca
     4921 aattgcttca tggcgccaaa ataattacca atatgtggcc gaccggttgg tttgacaccc
     4981 gacaggagga cttttttgtt gttttctggc atgggttaag tttagcagaa atatttgttt
     5041 cgtgaaataa ttggcgatat tgacaaaaac taattattta actaaagtat agaaaataat
     5101 cttaaccaag ctagcaatga ttaaataata acaacccaat acttttcgag tcgacattaa
     5161 tagtcgtttg gtttgttgtt atttatgcag aaaaattcta ataaaaagat tgccggtcag
     5221 attttggcgg tcaaaaatat ctctttgagt tttggtccac atccggtttt ggatgaggtt
     5281 tcttttgagg tgcaagctgg tgacaaaatt ggtttaattg gccccaatgg ttccggcaaa
     5341 agtagtctaa tgaaactttt ggttggccaa ctggagtcgg ataagggtga gattgtctta
     5401 tcgcccaacg tttcggttgg atttcaaccg caagaaataa tggaagatat gaccgctctt
     5461 gaatatttgg aagaaagttt gaactcgcaa gaaaacaact cctctcacta cgcgcttggt
     5521 ctgaatgatg aaattctggc aaaaaaattt agtgaaatgt ccggcggtga aagaagcaaa
     5581 atcgtcttgg ccaaaataat gtctttaaaa gatgatctcg ttttgcttga tgaaccgacc
     5641 aacaatcttg attactcggc cttgcaaaga ctggaaaaat atattaaaga ttcacgttca
     5701 acttttatta tcatttctca cgacagaaaa ttgctaacgg acttaactaa caaaattttt
     5761 gaaatagata gcgacaagcg ctccctctcg gtttttattg gcaattttga agaatatcta
     5821 aaagaaaaaa agcggtctaa gatcaggcaa tatgaaaaat ggcaagactt tcgcgatgaa
     5881 gaaaaaagat taacagtctc ggccagaaat aaatatgagt gggcggaaaa aggacaaaag
     5941 ccgaaaatta ccgaccgcta tcgtggcaat attggcttac aaaaagatcg ttcagcgcag
     6001 gtttttcatc ggttagctaa aaatttggaa cgaagacttg agaatttaga aaaagttgaa
     6061 aaaccaaaag gaaagttgcc gcaaaagttt tatttcaaca tcaatcgcaa gggtgaaggc
     6121 cggattttga aaatagaaag tcttgaaaaa aagttgccga acggcaagac ggttgggcct
     6181 ctgagttttg aggtcaattt gggagaacgt ttactgattt ccggacccaa cggcagtggc
     6241 aagacaacct taatgaagac tatcaagggc gagttgaagc cggactctgg caaaattacg
     6301 tttgccgaag gattaaaaat tagttatttg cctcaagact taaccgctct ccccggtcaa
     6361 acgactcttg atttattttt tgcgcaggtg gaactcccac aaactgaagg ccgaaattac
     6421 ttggtgcgat ttggtttgaa agaagaagac gtaaagaaaa aagtaggcaa tctttctccc
     6481 ggtcaaaggt caagactatt tttggcgctc gtcatggcgc aaagagcgga atgcctattt
     6541 ttagatgaac cgactaatca ccttgatttg gaggggttgg aggctttgga aaaagctctc
     6601 ttaggctacc ggggcggttt gatagcaatc agccacgacc gtcagtttgt gcaaaaaatt
     6661 ggtttcaaac aagaaattaa acttggttaa agaaaactcc cgcaacccta ggccgccttc
     6721 gcttttcggc tttgggccag gctatagata gtaaaaatat aggctggaat aataagcaag
     6781 aagctggcca gtgattgcca ttcgttttgt gaggggaaat aaatgagagc ggaaaatccg
     6841 acactgatag cgtcggcccc caaagcataa agttttagcc tttgttgttt gtttttgatg
     6901 accttcatgt tttggcaggt aaaataggcc caagccagca tataacccaa aatggtgaga
     6961 ccggtcagcc aggccgcgac cggattttgg ttccacaaga taactccatt ttctccggta
     7021 atgggtagac gcatatcaat caactgaatt gcaatgacga ttatgccaat caaaattatg
     7081 aagtttgaaa tgtagcgaga atattttttt atcaattgat tttcggaaaa agttggaatt
     7141 tcggtaatga aatatagcgc caaaaaagca aacaagacgg caaagttgta gcccaggatt
     7201 agggtgtaag gtttgttttg accgataatt gaaaaaacgg ccaaaattaa agcataacaa
     7261 gagaaaaaca gaaagatggc aaacaaactt ttgaccgcct gattggcgct acgtctggat
     7321 gaaccataaa gaaacccggt gatggtgccg agcaaaataa acattaatag aaataataaa
     7381 gaacctaagt aagtcattgt tttttgaaaa aataatttta agtttagccc gccttcgtgt
     7441 caaacgacat tggggcgggc gtgggggtta cttgcaaaac acgtaggtgt tggccggaac
     7501 gggataccgt cgccgcagag tgattcggct ttggagccgt tcctttctct gttttgtctt
     7561 cctttcttcc ctttttccca acacctcgcg cgcgctgagc catcttgcca attcgggcga
     7621 tggttttatc gctttcggtt tgtagggcgg aagctcaatc tccagacaga ttttagctgc
     7681 cgtcagttga gcttctctca caacgcggtc aagctctgac cgaccgacct cttttcggaa
     7741 ggtttttttg agcttgatta gctccgcgac agaaactgat tccccgtcag gcatggcgtg
     7801 ttgaattagc caatacctga gttgttgctc cagttctgaa accctcgaaa caagttcaat
     7861 tgtcataggc cccccgagtt tattgccctt gttaagttag tcgcttggtg acttggaaaa
     7921 ttccgcgtta agctatatct aatcccccat tgtttaagaa caaccccatt gttgtctgac
     7981 tttattttaa caatagttga gtttttgggc aaactgggga atttgattta aaaaaaggca
     8041 gggatttacc ctaccttaaa tcactagcca tttgttttaa gacagtttaa accttgctta
     8101 tttgtcgggt atcatgaaaa gaatcttaaa acttcgtatt gacggctgtt caaattgcct
     8161 agtatgatta aggagatgac aaaaaattct tcttgggaac acagttttta taaacaggct
     8221 tttttatgga tgtatcgcgc gcgcaatttt ccgagcaggg ttattttgcc ggttaactcc
     8281 tctggcaagt ttcagttgga agtccgggat tttggaacaa actatcatcg ccaaataacc
     8341 aaatcttcac aaggttcaat ttttggttca atttcacctt ctggtaagtt tgttttttat
     8401 cttaacgaca aaggcggtaa tgaaataggt cattttgttc gcgagccttt tagaggcggt
     8461 aaaaaggtgg atatcacacc aaatcttccc tcttattttg cttacgaagt tagttctgat
     8521 tacaatgacg aaagattgtg tttttatgct tcaatcaaag aaaaaaatta cgtctatcta
     8581 gtggaaacca ggcaaggaaa agaaggtttt aagagtaagt tactatatga gaccgaagat
     8641 cttattttgg ggaccgttat tagccccgat ggaaaacaaa catgtgtttc catctctctt
     8701 ggcggtaaaa aaaataaatc tcaagtttta gtttttgata acaataacgg taaattaatt
     8761 tgcaaaatta attttttggc tggagatttt atcgcccagg ccttttcggt tgactcagac
     8821 aaacctctaa tccttggaat tttgaacaag gacggttttt atcgtccggt ttggtttgat
     8881 tttctcaaga aacaaatcac cgagattaaa aaccctaaat ttaaaggtaa tgtttttgtt
     8941 ctcgagtggc tggaggacca aaataaaatt ttatttggtc gggaatataa acttaaacac
     9001 tctcttttgc tttatgattt aaataaaaaa acaattttgc caattggacc tgattatgga
     9061 agccttgatt tgttttttgg ttcagttact tctcttctgg atggttctct tatattgcgt
     9121 tggcaatctt ttgatattcc accgcaaatt attaagattt ttgatcataa ttaccagaag
     9181 acgaaaagtt tatatttagc caagagacag attcgtaaaa aaaaactttt gaaagtgtgg
     9241 ttttcaagtc ttctgatggc gcacaggtcc aaatgtgggt ggcttggcca acaaaaaagc
     9301 aaaaacttta tccttttgtt atcgacatcc acggaggtcc acatggttct gtcaatgatt
     9361 ctttttctcc cgaggctcag attttattag acgaaggctt tggttattgt gctgtaaact
     9421 atcgcggctc aactggtttt ggtaaagatt ttgagcgagc tatttatggc aatcctggat
     9481 actttgaggt agaagatatt gtttcggctc gggggtggct tatttccaaa ggtcaggctg
     9541 atcctgataa aattatttta tttggttggt cttggggagg ttatgtcact cttttagctc
     9601 ttgggaaata tcctaattta tgggccgggg gtattgccgg gactccaatt ggtgattatc
     9661 tttcacaata tgaagatgag ccggctttct ttaaagcgat tgaccgggag ttgtttggag
     9721 gcagtccgga gcaaaaaccg gctgcttata aaaaaagttc acctattacc tatgttgata
     9781 attttaaaaa acctgtcttg gtgatttatg gagaaaatga tgcccgttgt ccaaccaaac
     9841 aaattgttaa ttttattaac aagcttaagg caaaaaacaa acttattaag gttaaaaaat
     9901 tttcgtcggg acatacgggc gaattttcta ataccaaaat taggatttca aattttaaag
     9961 agattctaaa gtttgctaaa aggttaacaa ggtaagtaaa aaatccggag agttatgcta
    10021 ttggcatact accctccgga ttaatcctgt ttggacagga ggcgaacagt tatgattccc
    10081 tgttgtgctt gacggggctc atcaggagac tgttccggtg cgaccaagtg tgcgtctggg
    10141 gatcgtaact ccccatttcg ctggctgggg ttgatggcgc cattctcagg aggcgaccaa
    10201 tcatcgttgt ctgtactatt gttgttctcc cttgagaaag gtttttgggc ctatcccaaa
    10261 aatctgtaat ttacgatacc atacctaaga tttatagcaa gttttattag taacgggtgt
    10321 aaacattacc tcaatctatt gtgtaaaact ttaaacgatg ctattctttt caagagaatc
    10381 tcattgtggg ctcttggaaa tctaggaaga aggaggtgag attgatgaat gaaattctcc
    10441 ttatccccga tgccgaaacg cgcgcaagag tcgctgagaa aatcgacttc aggaacgcag
    10501 ttgatgctcg accctgcgat tgcactgcct gcaattcctg tcgctgcact ccgtgcaagt
    10561 gattcggcga aacacagacc taagcatgtc tataaactgc ttttttatat tcaaaaaatt
    10621 ggtaatatat tgagaatttg atatattttt agtatatgaa aaaacttcgt ttctgcacaa
    10681 acaaacagct tgacgagata atgattgagc agtttttgca tgagcaaggt ggcggaatag
    10741 attttggcaa aggggttatt aaactgcatc caaggttaaa attagccaag tcgctcgaaa
    10801 atgttgaaca gaagaaaaag ataattcatc tttatattca taaatattac catagtcaca
    10861 aagaagaaat aaattacagt atcaaaaatt tacaaaaagc ctggcataaa aaagaaaaag
    10921 agtttgtgat agaaactgaa aagtttttca gcaactttcg gtttcctgaa ggaaaatata
    10981 ttgcgtatgc ttccatagtt gattgtaatc ctcgtttttt agattctaaa acttttcaat
    11041 ttttctacaa aaaaaagtta gcagatgcaa ttcatactat tgttcacgaa ctccttcatt
    11101 tcattttttt tgattttgtt gaaaaaaaat taaaaagtga aataaaaatt ttgacagaag
    11161 aacaactgtg ggatctttct gaaatattca acgtggttgt tcttggatct gctcagtatc
    11221 aaaatattgt tgataaaaag tatgtatttc cataccctca ccataaaccc ttactttctc
    11281 aatttagaaa ggcgtataag gattcttgta atgccgaaga atttattaaa cgaggcgtaa
    11341 ctattataaa gacaaaaaaa tagtgggagg atgctgtcct cccacttacg ggtttcttta
    11401 tttgtgactc acttttgttt ctgtttctcc cacaagtcac gaataagaaa gtgggttgtc
    11461 gctatcgcgt gagcacagaa tcgttcgtca agagcatcaa tactcccttt gattcggtgg
    11521 gcgttgtacg gacaacctcc accacaatta ccgagagcga tgcaagtttt acatgtctcg
    11581 ttcgcgaaag gtgaacgttt acgccattgc ttccaaaagg agtgagactc cggatcgaac
    11641 gtttcaaatg gctcagtaac gaagaattcc ttcttaccac agaaagcttg gcaagtacca
    11701 atctcgccgt ttgggcttac tacgatctga agtccgcagc ctccgcagtc ggaaagtacc
    11761 ggctccttgt caagaaaatt cttgactcgg cgcatcatgc ggtcctcgta gataccctct
    11821 gttcgtgcca ccctgaaact cttgatgagt tcctgggcaa caatctcggc atagcgatcg
    11881 tgcttttctt cagcttgtcc cacagaattc tcaatgagga tgttgaatcc cattcccttt
    11941 gcgccgagtt cgttagcgag ccagtgtaca atttcctcca tccgaaaaag gttgtgttga
    12001 tcaatggtta cgcaaatacc agttttaacc cccttgtctt gggcgagtcg gtatgagcgg
    12061 aggacttctt caaaggtgcc ttttccggaa gggtaaacac gcatttggtc gtggacatct
    12121 tttgggccat caatggaaat ggccaccacc actccgcttg atttgcactg gttaagaatt
    12181 tcctcatcaa gaagaatccc gttggtgttc aaggtcaccg aaatcgtctt tggcaagagc
    12241 ccttcttctt tcagcgcgtc aatatatctg agcgcatcca gaagaactcc tttgttgatt
    12301 gttggttctc ccccatagat cacgatgtgt tgttctggat gacctttggc aatgcttttc
    12361 tggacacagc gggcaaagag atccagcgcc ttgatcgcaa cctctttttt catgacggag
    12421 aatcggtagt cgtaaggcat cggtgctaga aagtagcagt atcggcaacg aatgttgcag
    12481 gcatcggaaa gaaggagata gaggatgttg attgatggct cctcaagtgc tttttggtac
    12541 tcacgatacc gctcttcgcc atcggcttgt tcctttataa ggagcccaag ctttaccaat
    12601 gattccagta catgttttga actgacatca gaggcaatcc acgcctggct atgtcgatgg
    12661 cgatcaatga agtcttcttt gagaaatgcg acctctaggt tcagactgtt gtagagggct
    12721 acatatccgt tatctagagg aaccaaatga agaaatggac tctgtttaag tttcatgtgc
    12781 ctcgtctcct gctggttttg aaggttctaa tttgcaaagt tcggcatacg tcagttccac
    12841 acttactata cgtattaagt aaagaaagtc aatgttggtt tatttgactt gatcacccca
    12901 aaaccgcccc agtttaagga tttaaggaaa aaatcttttg acaatgattc ttagtgtgtt
    12961 tatagttaaa caaaatgtct ctaaacaaag aactaaaatt aaagatagaa gaaatgacaa
    13021 gggttgatca aaaccttaga aagaaacaat ggaaggaatg gaaaaatttg gttagggaaa
    13081 ataccaataa aaatgaccct aaacttttgg cgatggtcca gaaaatggct aaggagttat
    13141 tgagagttga caagaaaaac actcttgaga tgaaaaagat aatcagcaaa tatggttggc
    13201 ccgggaggtc tctagttggc aaaaaaggag ctctcaatgc ttggcttttg gttcagcatg
    13261 ccgaccatga tgttgatttt caagagaaat gtctaaagct tttaactgcc gcggcagaaa
    13321 agggcgaagt tgataaaaaa agagtggctt atctgacaga tcgggttttg ttgcatcgcg
    13381 gcaaaaaaca agtttacggc acccagttga gaagttttcc tcaaaaagga aagacgattt
    13441 taaaaccaca accgataaaa aatagaaaaa aaattgatga actgcggaaa ggtatggggc
    13501 ttgagccatt gaagatttat ttggataaaa tgagaaaaat gtattgatac tgtttttaaa
    13561 catttattct cttgtatcac ctcgtaactt atcgttttta gattttagaa ttagtaacat
    13621 taaaaaatcc cctccacttg agcaaaactc aggtagaggg gaaacgtgcc taacgtccgt
    13681 tcttgagcag agttgcgatt gcttctgcta tagggagttg ggtaagatct tcaatccagc
    13741 cccactgtcc tgccggattt acttctaaaa atacataacg gtcatctggg gttataacca
    13801 tgtcaattgc gccgaacttt aatccccagc tcctcatgca tgtcaggagt ctgtctttga
    13861 cgtattgggg tagttcaaat acctcgtgct ttacattgac gaagtcgtac tttctccaat
    13921 catgctttgt cctgtcgcta tcttgagagt ggattgcaca ggcaaaaaca ttgtctccca
    13981 ctaccgtaac ccttagctcc aaccttttcg gtatatagct ttggagaatt actgggcaaa
    14041 gcttcaactc atcgaggtta gcttcaaggt cttggagggt aatcggggtg gtgtaaactc
    14101 ccataggcca tccttcttca tcttttgaga atgtcccaaa ggtcttcaag atgcaagccc
    14161 cgtgttttga gagaaagtcc cgtgctcttt cgtggtcgtt agtgacgatt gagtttggga
    14221 catcaaggcc tgccttgcag gcttctttta tctggagttg tttattacac tctatcttcc
    14281 aactcaaata aggatggttt acccaaagaa cattgtgggg gtactgagaa ttccataagg
    14341 ctcttaagaa cctggacact tcttgttcgg catattgctt gtctgcaagc tcaagatcac
    14401 ccatcccctg ttgcttcttt ccaggccgtc ggtaccagat agagctaata tcagaagatt
    14461 caattcttga ggagtcgtca gtaattgaga atgtatcagt tcctgaactt gtctcttgag
    14521 taacgccgta gttttcaaaa taatcggtct tgaaaacaaa ggtgtcttct cgagggagaa
    14581 agttcaaaac actctcaaca tcagagactt ccgatgatgt gactactaga attttgcttc
    14641 gcattgcgag atctcctttt ttatgaacga actctcccaa cgaactttga agttgaacag
    14701 ttaatgtatt aaattaactg tcatatg
//
LOCUS       DAOOLO010000014        14567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_4440_length_14567_cov_6.181298, whole genome shotgun sequence.
ACCESSION   DAOOLO010000014 DAOOLO010000000
VERSION     DAOOLO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4440_length_14567_cov_6.181298"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLH96_01475"
     CDS             <1..184
                     /locus_tag="PLH96_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60474.1"
                     /translation="ILALTYAKNNLYSISSDPQIIARREDKKFGKTTKVGDMLRRKTK
                     LNLTVFLVEENKIFLF"
     gene            complement(531..617)
                     /locus_tag="PLH96_01480"
     tRNA            complement(531..617)
                     /locus_tag="PLH96_01480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Ser,seq:gga)
     gene            complement(595..1056)
                     /locus_tag="PLH96_01485"
     CDS             complement(595..1056)
                     /locus_tag="PLH96_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HPB60475.1"
                     /translation="MPAIRKCKGSNTLVISLASTSVVDFLVRNGLPRGSKLKNGLHIP
                     EWILKKKIYKRACIRGLIDTDGCVFIHIHNVRNKTYKNIGLTFTSYSRDLIFQVATIL
                     EEFGIMPHISKRGQDIYLYQADSVLKYLKIFGTSNNRIRSVYNQWRDAGVV"
     gene            complement(1412..1768)
                     /locus_tag="PLH96_01490"
     CDS             complement(1412..1768)
                     /locus_tag="PLH96_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60476.1"
                     /translation="MEQIPPRESKKFDSLAELRNHLESVYQEEKMVANVSHLTFGEDF
                     DPYELGEADLEVWNLAQDYFGNVISFDELMGVFRAYQLSLFNENFMASSRGKFSSYLS
                     NMITKRHLEESRPPNR"
     gene            complement(1804..2700)
                     /locus_tag="PLH96_01495"
     CDS             complement(1804..2700)
                     /locus_tag="PLH96_01495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPB60477.1"
                     /translation="MATLYTEQDKNTRRTWLLMAGFLAVIVALGWFISDYYNNSAILY
                     AAIIFSVSMNVASYWWSDKIALSFNGAKEVAREEDVELHRLVENLAITAGLPKPRVYV
                     IEDLAPNAFATGRDKNHAAVAVTRGLLNMLDKNELEGVVAHELAHIGNRDILLQTVVV
                     VLVGFVAIISDMFMRLSFSGGDRDRNRGGNLLAIVGVVFVILSPIIATLIQLAISRQR
                     EFLADSSGALLTRYPEGLANALQKISGYGGTLKRANNATAHMFIANPFGPKAARGVSK
                     LFMTHPPVEERIKALVGGKTKI"
     gene            complement(2703..3260)
                     /locus_tag="PLH96_01500"
     CDS             complement(2703..3260)
                     /locus_tag="PLH96_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB60478.1"
                     /translation="MIIFWIILALVAVVAIWLAVAYNSFISLIARTKEAWADIDVQLK
                     RRYDLIPNLVETVKGYATHEKEVFERVTEARAKALSAEGPVAKGEAENQLSGALKSVF
                     AVAEAYPQLRAVESFASLQAELADTENKIQAARRFYNGNVRDLNIKIESFPSNIVANL
                     FKFEKREFFELEEGNEAKEPVQVKF"
     gene            complement(3424..5070)
                     /gene="groL"
                     /locus_tag="PLH96_01505"
     CDS             complement(3424..5070)
                     /gene="groL"
                     /locus_tag="PLH96_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411268.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPB60479.1"
                     /translation="MAKQITFNDDARKALKRGIDQVADAVKITIGPKGRNAVLDKGYG
                     APTITNDGVSIAKEITLADKVENMGAEIIKDVASKTNDIAGDGTTTAVILAQAIIEEG
                     FKKTALGVNAMGVKAGIEAAAAKVVKELKAIAKPIKDKHEIVQVATISAESEEFGKII
                     ADAIEKVGKDGVVTVEESQSFEVESEFVEGMQFDKGYVSPYMITDAERMEAVYEDPVV
                     LITDKKVSTVKEILPLLEKLAGAGRKEMVIIADDVDGEALTTFVVNKLRGTFNVLAVK
                     APGYGDRKKEMLVDIATLTGGTLISEEVGIKLENVEPNMLGRARRVIATKDGTTVVGG
                     KGKKADIEERVATIKKQIEASDSSYDKDKLRERLGKLSGGVAVIKVGATTEAEMKYKK
                     LKIDDAVAATKAAVDEGIVPGGGTALIKAGAKVAASKIAAPSEDIAAEFQAGVEILLD
                     SLKAPIEQIVLNAGKNNAAVVIDKILSGKANEGYDANADAVAEDMFKAGIIDPVKVTR
                     TGLERAASAAAILLTTEVAITEEPKKEEPHNHGAEMGGMY"
     gene            complement(5124..5435)
                     /locus_tag="PLH96_01510"
     CDS             complement(5124..5435)
                     /locus_tag="PLH96_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB60480.1"
                     /translation="MAKINIKPLGDRVLVEIKKEDDRTGKTKSGIIIPETVSKERPEE
                     GVVIAVGEGRRLDNGTLVPLSIKKGQKVIFSKYGPDEIKVGDKDYLIVGESSILAVIE
                     D"
     gene            5797..6027
                     /gene="secG"
                     /locus_tag="PLH96_01515"
     CDS             5797..6027
                     /gene="secG"
                     /locus_tag="PLH96_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB60481.1"
                     /translation="MSLLSSYLPWIQIILSALLVGAVLLQQNEAGLGNSFGGGQAGAP
                     AHTKRGLEKQIFNATIIIAVLFVASTMLALFI"
     gene            6170..7897
                     /locus_tag="PLH96_01520"
     CDS             6170..7897
                     /locus_tag="PLH96_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB60482.1"
                     /translation="MPSKLKTVLFKETRFAFDSLPPRLKALFVVLFIFFVGSLGVLVW
                     RLNQNFIVTVPTEGGSLTEGVIGPARFVNPLLAVSDADRDLTSLVYSGLVRVGPKGNF
                     IPDLAKSYEVSEDKLTYTFHLKDNLRFHDGAPLTADDVRFTIEKAMDPAVKSAKRANW
                     NGVSIEQPGPKTIQLTLKKPYPAFLENATLGVLPSHLWQNTSAESFPLHEQNLEAIGS
                     GPYKLSKITKDSNGIPTAYELKAFSDFALGKPRLEKITIGFYKNEEELVEAYKNGQID
                     SLSSLSPALVKELEESGQRVVKSTLPRVFAVFFNQNHNSVFLDATVRKALNLAVPRQE
                     IVENIFHGYAEPLEGIFTSTNGEPTKTDLAAAKKMLTDAGWKLNDQGVMQKTTKASKD
                     KPAATQVLTFSLATSNIEELKQIAEKLKANWTALGAKVEIEIYESNDLNQNVIKPRKY
                     DALLFGEVMGRDSNPYPFWHSSQRTDPGLNIAMYTNAKVDRLLEEARQTSDLEERNKK
                     YFEAETEIKKDQPVAFIYSPYFLYLLPSNIMGVSLPTLTNPAERFVNIHKWYVRTDKV
                     WKIFANLYK"
     gene            7959..9854
                     /locus_tag="PLH96_01525"
     CDS             7959..9854
                     /locus_tag="PLH96_01525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPB60483.1"
                     /translation="MPIKRIRVDASGNIQTFVADKKTEKVGRPSGKMGGQRFSPNKKT
                     TGRGHSPRKTSPSKTQSEDNKPKQKIPPVAPGVIRIIPLGGVEEVGKNMTVVEFGNDI
                     VVIDIGLTFPGEEAPGVDYIIADPTYLEEQKSKIRAVIITHGHLDHIGGIPYVMSRIG
                     NPPIYTSLLSAVMIKKRQEEFPHLPALNINVVSEKDKIKLGNLSIRFFATTHTIPDSI
                     GVIIETPFGNVIFTGDLKVENTNGVPEKFEEERFNKLGQENNLLLVADSTNVEKPGFS
                     YPEKEVQKNVKEIIANAKGRVIIGTFASLFDRIIAIIEACEELGKKVAIEGRAMKTNI
                     EIAKELGYLKTKRGTFATPDEMATLPENKIVILATGAQGDEYAALMRISQKEHKSVKI
                     KKGDTILLSSSVIPGHEKAVQRLKDNLARQGAKIVHYGIANVHSSGHSYQDEIAWVNK
                     KLKPRFLLPVHGHQFMLHVHANVAHEIIGMPEKNIIVPENGSIIEISDKGQKISALKQ
                     MASAKLVMIDGLGVDNVQEVVIRDRQMLAQDGMFVLIAIIDIKTGKVRKSPDIISRGF
                     VYLKESQDLLRYVRLLTKKTIEDSTAQMHPINLDYVKNNVREELGKYLFQKTHKRPII
                     LPVLIEV"
     gene            9929..11101
                     /locus_tag="PLH96_01530"
     CDS             9929..11101
                     /locus_tag="PLH96_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB60484.1"
                     /translation="MRSQKTLILFTYLCGLTMTFSFAFPGYVNSTFLENFVGEKWVGF
                     IFAIGALLSVILMSQISKLIKAYGNIKVLVSSAALAIFVLILLALAKSVTVIISTFII
                     YYCLGFFIRYTLDVYLENISDDKNTGLIRGFYLTFLNTAWLISPFLASRLVVNGDFWK
                     IYVVAAMSLLPLIAIASVFLYEQKDLKYDGQSIFPQLKRLWRGENQTDRCIRRILIVD
                     FLLCLFYAVMVIYMPLYLHEHLGLPWNELGLVFTIMLVPFVILELPLGRIADRWMGEK
                     EILMGGLVITGLSTSVLAFIPSASWLVWAGLLFITRVGAAGIEVMKEAYLFKKIDGSD
                     TSLISLSRIIVPFSYLVGPLFVSLILLFFDFNYVFFFLGLLVLSGLFFATKLKDTK"
     gene            complement(11138..14128)
                     /locus_tag="PLH96_01535"
     CDS             complement(11138..14128)
                     /locus_tag="PLH96_01535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPB60485.1"
                     /translation="MQGVEKAWECYNLGMNAQMARKWEEYQARFNEAYNRLNSAQKEA
                     VDTIEGPVMVIAGPGTGKTQILALRVANILYQTDTPPSGILALTFTEAGQKAMRLKLR
                     QFIGSAADEIGVYTYHGFASAVMAEFGDHFPHLRQAKQLTEVEAETLIREILRAKRFS
                     RLRPLGDPDFYVGKIIKAISDCRKEAWTPEMIADFAAAQIEVIKNDPASISSRGATKG
                     QLKADALKKIDKAEKTKIFAEVYATYEEKKKIERRIDFDDLIFELTQALEQDELLLRL
                     LQEKYLYILVDEHQDTNDAQNALIKKIADFYEEPNLFVVGDEKQAIYRFQGASVQNFL
                     RFQSLWPAMKVISLENNYRSHQGILDACFGLIENNYQADEYPELRIRLKSANGEEPEP
                     VEVVMAGNVAAAEKYLADEIKKVLMADDKATVAVITKTNRDVENALRVLETAGVPAAA
                     ERGADVFGHPLGRLFFALAEYLADAAKTESLAYALSGGLWGLDLFERAEVMKNIRSGN
                     LKDLDKKLSKLSVLKEKAVSVSAVEFLILVGEESGLVALTLGDPLSMEVWRSIVALGE
                     DLARTQKIEDPILLIEAMLSFRQTAENKSVKIMAGFGEARVRIMTAHGSKGLEFDYVF
                     LPYATEESWMSRGFGNYFLLPESKEENDDLRDARRLFYVALTRARHRAVVVVGLADGM
                     KRTLTPLRFIDELDQENVARTSLPALVTAPAAVNLSQLAKKDEARLVEFAKNILLEKG
                     LSVTALNHFCECPSKFFFKSILKVPEAPAMPAEKGNAMHEAIAAVWALSEKTEEKITE
                     TLKVALQNYFAHSLLLSADKELALEELLLAAPKVAAALGPHFAIKGVAAAEKWVEARF
                     EFKVEKQAVELKIHGKMDAIIDTAEKVLVFDYKTKSAMSEAAIKGETKNDDGNYFRQL
                     IFYKILLAESSFYRGQTIEPALVFVKPDNKGRCPTVSIPIAAEDISRVKGEIGALLES
                     VWTGRILTETCDDPKCEYCQLMNL"
     gene            14355..>14567
                     /locus_tag="PLH96_01540"
     CDS             14355..>14567
                     /locus_tag="PLH96_01540"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPB60486.1"
                     /translation="MLHQQIKDEIKKAMLARETERLMAARNILAAFTNELVVKKQRPD
                     EMLTDDALAVLKRLAKQRKDSIEQFTI"
BASE COUNT         3998 a         3669 c         2770 g         4130 t
ORIGIN      
        1 aatccttgcc ttaacctatg caaaaaataa cttgtattca atttcatccg accctcaaat
       61 tatagcacga agagaagata aaaagtttgg aaaaacaaca aaagttgggg atatgttaag
      121 aagaaaaaca aaattaaatt tgactgtttt tttggttgag gaaaataaaa ttttcctatt
      181 ttaaaaatac ctcaagctgg aagccttaaa cagctcaacg tattgagccg tttaagactt
      241 tattccctga agcaagaaaa tgatggtttt ttctctctgc caaaggtggt ggccaagagg
      301 gcacccttgt taatattccg agcgaagaga gaggggttcg gtcacgagtt actaagtctt
      361 ttgctcggat ccgccagctg gcggactcgc aaaagtcaaa gtactctcga ccccggctcg
      421 attctcttcg cttcaatcag tctgaatctg cgcccttcaa atccctcctc tcaaaaagct
      481 gccaattttt accatccttt caagttttcg gtatgaaaaa attaccagtg cggagagaga
      541 gggattcgaa ccctcggcac ggtttcccgc gcacagactt tccaggtctg ccagttaaac
      601 cactcctgca tctctccatt gattataaac agatctaatt ctattattgg atgtcccaaa
      661 aattttcaag tactttagaa cagagtccgc ctgatacaaa taaatatctt gtcctctttt
      721 actaatatgc ggcataattc caaattcttc aagtattgta gcaacttgaa aaattaagtc
      781 ccttgaataa ctagtaaaag taagtccaat atttttatat gttttatttc taacattatg
      841 aatgtggata aatacacacc catcagtatc tatcagtccg cgaatacaag ctcttttgta
      901 tatcttcttc tttaaaatcc attcgggaat atgtaaacca ttcttcaact tactccctcg
      961 aggtaaacca tttcttacca gaaaatctac tacagacgta cttgctagtg aaatcacaag
     1021 ggtatttgac cccttgcatt ttctaatagc gggcataata ttaaatagca ttttacaaag
     1081 aaccaaaatg taatctgagt actctaaatc agacagcgtt tactgaaatg tttgcttgcc
     1141 aagtattatt tacaccccca tcacctatca taataccaaa aaattccgct aaattttggg
     1201 aatattttgg aatagttact ttctttcgat gacttttttc agggtatcta agttcttgaa
     1261 cattgaccac tggtaaatta taacaggcct agtacaaatt actactattt gcactaggac
     1321 attatgcgaa ccctccaaat ctgaagttat cctaacagat tttcgacaaa tttcaaaagg
     1381 ttcgtaaaaa tcttgtttga cgtttttttg ttcatcgatt cggtgggcgg ctttcttcca
     1441 gatgtctttt tgttatcata ttagataagt agctggaaaa tttccctcgg ctagaagcca
     1501 taaaattttc attgaataga cttaattgat aggcgcgaaa aacacccata agttcgtcga
     1561 acgaaataac gttcccaaaa taatcctggg ccaaattcca tacttccaaa tcagcttccc
     1621 ccaactcgta tgggtcaaaa tcttcaccga aagtcagatg actaacattg gcgaccattt
     1681 tttcttcctg gtagacgctc tccagatggt tacgcaactc tgccagtgaa tcaaattttt
     1741 tactttctct tggtggtatt tgttccatcg caaaaattta aaaattaaaa aagctcatca
     1801 aaattatatc ttagtcttac cgccgactaa agctttgatc ctctcttcca ccggcgggtg
     1861 agtcataaat aattttgaaa caccgcgcgc cgcctttgga ccaaaaggat tggcaatgaa
     1921 catatgcgcg gttgcattat tggctctttt aagcgtcccg ccataaccgg aaattttttg
     1981 caaagcgttc gccaaacctt ctgggtagcg tgtcaaaaga gcgccggagg aatcagccaa
     2041 aaattctcgc tgacgcgaaa tcgccagttg aatcagagtg gcaatgattg gtgacagaat
     2101 tacaaaaaca actccaacta ttgctaaaag attcccgcct cggttgcgat cccggtcacc
     2161 gccggaaaaa cttaagcgca taaacatatc agaaataatc gccacaaaac caacgagaac
     2221 taccacgacc gtctgcaata aaatatctct attgccaatg tgcgccaact catgggcgac
     2281 caccccttcc aattcattct tgtctaacat atttaaaaga cctctggtca ccgctaccgc
     2341 cgcgtgattt ttatcacgcc cagtggcaaa ggcattgggc gcgagatctt ctatcacata
     2401 aactcgtggc ttaggcaaac cagcggtaat agccaaattt tccaccaagc gatgaagctc
     2461 cacatcttct tcgcgagcca cttctttggc gccattaaac gatagagcta ttttgtcaga
     2521 ccaccagtag ctggcgacat tcattgaaac gctaaaaata atcgccgcat acaagattgc
     2581 cgaattgttg taataatcac tgataaacca accaagagca actatcaccg ccaaaaatcc
     2641 cgccatcaat agccaggtgc gtcttgtatt tttatcttgt tcagtataaa gggttgccat
     2701 tactaaaatt taacctgaac cggttctttg gcttcgttcc cttcttccaa ttcaaagaat
     2761 tctcttttct caaatttaaa caaattcgcc acgatgtttg acgggaaaga ttcaattttg
     2821 atgttcaagt cgcgaacatt gccattatag aatcgtcgcg cggcctgaat tttgttttca
     2881 gtgtctgcca gctccgcttg caaactggca aagctttcca ccgcgcgcaa ctgcggatag
     2941 gcttccgcca ccgcaaaaac gcttttcaag gcgccagaca attggttttc cgcctccccc
     3001 ttcgccaccg gaccttcggc tgacagagcc ttggccctgg cttcagtcac cctttcaaag
     3061 acctcttttt cgtgagtcgc gtaacccttg accgtttcca ctaaattagg aatcaaatca
     3121 taacgtcgct tgagttggac atcaatgtcc gcccaagctt ctttggttct ggcaatcaaa
     3181 gaaataaacg aattgtaggc cactgccaac cagatggcta caaccgccac cagcgccaaa
     3241 ataatccaaa aaataatcat agtttgtatt aataaatgaa taaaccccga ctcatttatt
     3301 ataccacttt atcaagcaag caactaatta ttgtttacaa aaaacgagcc ttgggctcgt
     3361 tttttgtaat tgttatttgt ttccgccacc ctcctttttt gtttatattt gggcttgaac
     3421 cttttagtac atcccgccca tctcggcgcc atgattatgc ggctcctctt tctttggctc
     3481 ttccgtaatg gccacctcgg tcgtgagcaa aatcgccgcc gcgctggccg cccgctccaa
     3541 accggtgcgc gttactttga ccgggtcaat aatgcccgcc ttgaacatat cttccgccac
     3601 ggcatccgcg ttcgcgtcat aaccttcgtt ggccttgcca gacaaaatct tatcaatcac
     3661 caccgccgcg ttgtttttgc cggcgttcaa aacaatttgc tctatcggcg ccttgagtga
     3721 gtcaagcaaa atttcaaccc ccgcttgaaa ctcggctgcg atatcctccg atggcgcggc
     3781 aatcttgcta gctgccacct tagcgccggc tttaatcaaa gccgtgccac cacccggcac
     3841 aatcccttcg tcaaccgccg ccttggttgc cgccaccgcg tcatcaattt tcaatttttt
     3901 atatttcatt tccgcttcgg tcgtcgcgcc aaccttaatc acagccacac caccggaaag
     3961 cttgcccaga cgttcacgca gtttgtcttt gtcatatgac gagtcagagg cctcaatttg
     4021 ttttttgatg gtcgcaacgc gttcttcgat gtcggctttt ttacccttac caccaacaac
     4081 cgtcgtgcca tctttggtgg caatcactcg cctagctcgt cccagcatgt tgggttcaac
     4141 attttccaac ttgataccaa cttcttcaga aataagagtg ccgccggtca aagttgcgat
     4201 atctaccaac atctcttttt tgcggtcacc atagccgggc gctttaaccg ccaaaacgtt
     4261 gaaagtgccg cgcaatttat tgaccacgaa agtcgttagc gcctcgccat ccacgtcatc
     4321 agcaataatc accatttcct tgcggccggc gccggccaat ttttccaata aaggcaaaat
     4381 ttccttaacc gtggaaactt ttttgtcagt aatcaagacg accgggtctt cataaaccgc
     4441 ctccattcgc tcggcatcgg taatcatata gggcgaaaca taacctttgt caaattgcat
     4501 cccttccaca aactctgatt ccacctcaaa agattgtgac tcctccacgg ttaccacacc
     4561 atctttgccg actttttcaa tcgcgtcggc aataattttg ccaaactctt ccgactcagc
     4621 tgatatggtt gccacttgga caatctcatg tttgtctttt atcggcttgg ctatcgcctt
     4681 gagctccttg accaccttgg cggccgcggc ttcaattccc gctttgaccc ccatcgcgtt
     4741 cacaccgagc gccgtctttt taaaaccttc ttcaataatc gcttgcgcca aaatcacggc
     4801 cgtggtcgtg ccatcgccgg cgatatcatt ggttttgcta gccacatctt tgattatctc
     4861 ggcgcccata ttttcaactt tatcagcgag cgtaatttct ttggcaatag aaacaccatc
     4921 gttggtaatc gtcggcgcgc cgtaaccttt atctaaaacc gcgttacgac ccttgggacc
     4981 aatggtaatt ttaacagcat cggccacttg gtctatacct cttttgagcg ccttgcgcgc
     5041 gtcatcattg aatgtgattt gttttgccat agtaaaatta aaaattaaaa tataaaattt
     5101 ataaggccaa tcaacaaaac agactagtct tcaataacag ccaaaataga agattcgccg
     5161 acaatcaaat aatccttatc gccgaccttg atttcatcgg gaccatattt agaaaaaatc
     5221 accttttgac cttttttaat tgacaggggg accaaggtgc cgttatccaa gcgacggccc
     5281 tcacccaccg caattaccac cccttcctcc ggccgctctt tggagacggt ttccggaata
     5341 atgatgccgc ttttggtttt accggttcgg tcatcttctt ttttgatttc cactagaaca
     5401 cgatcgccca atggtttaat gtttattttt gccataagcc ataatgaaat ttaacgtgac
     5461 actaatatat taatgaatac aaacagagaa aataagtaaa aagttaaaat aagaagattg
     5521 agaaaataat aattcttttc acattgttac atatctgaag aagctcttat taaaacgaat
     5581 aaagagctta tccttatttt agcttgtaaa gatgctttga attcgacccc aaagcaaatt
     5641 ccttttatta taacccaaaa aaggccaaaa tcaagagcag tctaaatcac cctaatatca
     5701 agtagctcct cgcggcaaaa ctcgacttgc tcttggtacg taacctgtgt ttagccttgt
     5761 tgacctcctc ttttaatttt gctatactct ttctcaatgt cgcttttatc tagttatttg
     5821 ccttggattc aaataattct ttccgccttg ttggtcggag cggttttgtt gcaacaaaat
     5881 gaggccggtc taggcaactc tttcggcggc gggcaagccg gcgccccagc ccacaccaag
     5941 cgcggtctgg aaaaacagat tttcaacgcc actatcatca tcgccgtttt gtttgttgct
     6001 tcaacaatgc tagctttgtt tatttagaag ttttttctca aaatcctctc ttttttaata
     6061 aggtttcaat ccgaaatatt ccctatttta atttcttttc tttattcgct taatttatgt
     6121 aacttattat gtaacttagg atattagtgt catatcaaat tttgtattta tgccatcaaa
     6181 actaaaaaca gttctgttta aagaaactcg cttcgcgttt gacagtttgc cgcctcggct
     6241 aaaagcccta tttgtcgttt tgtttatctt ttttgtcggt agtttaggtg tattggtttg
     6301 gcgactaaac caaaacttta tcgtgacagt gccaaccgaa ggcggttcac taacagaagg
     6361 ggtcattggt ccggcgcgct tcgtcaaccc gcttttggct gtttccgatg ccgaccgtga
     6421 cttgacttcc cttgtttact ccggtctggt cagagttgga ccaaaaggca atttcattcc
     6481 cgacttggcc aaaagttatg aggtttcaga agataaatta acttacactt tccatctaaa
     6541 agataattta cgctttcatg acggcgcccc gttaacggcc gatgatgttc gttttacaat
     6601 tgaaaaagca atggacccgg cagtcaaaag cgccaaacgc gccaattgga atggcgtttc
     6661 aatagaacaa ccaggcccaa aaacaatcca attaacttta aagaaaccct acccagcctt
     6721 tttggaaaac gcaactcttg gtgttttgcc aagccattta tggcaaaaca cttcggctga
     6781 atccttcccc ctccacgaac aaaatctgga agccatcggt tctggtcctt acaaactttc
     6841 caaaatcaca aaagattcaa atggcatacc aaccgcttac gaattgaagg ccttttctga
     6901 ctttgccttg ggcaagccgc gtttggagaa aattaccatc gggttttata aaaacgaaga
     6961 ggagttagtt gaagcttaca aaaatggaca gattgatagt ttgagctctc tttcgccagc
     7021 ccttgtcaaa gaactggagg aatcgggtca gcgagtcgta aagtccaccc tgcctcgagt
     7081 ttttgctgtt ttctttaacc aaaaccacaa ctctgttttc ttggacgcga ccgttcgcaa
     7141 ggctttaaac ttagccgtgc cacgacaaga aatcgtggaa aatatttttc atggttacgc
     7201 tgagccgctg gaaggcattt tcacctcaac taatggtgaa cctacaaaaa ctgacctagc
     7261 tgccgccaaa aaaatgttga ccgacgccgg ttggaaactc aacgaccaag gcgtgatgca
     7321 aaaaacaaca aaggcctcta aggacaagcc ggccgctact caagtcctga ctttttcctt
     7381 ggccacctcc aacattgagg aactcaaaca aattgccgaa aaattaaaag ccaactggac
     7441 cgccctcggc gccaaagtgg agattgaaat ttacgagtca aacgacctaa accaaaatgt
     7501 catcaaacca cgtaaatatg acgccctctt gtttggcgaa gttatgggcc gcgacagcaa
     7561 cccataccct ttttggcact catctcaacg caccgacccc ggcctgaaca ttgcgatgta
     7621 taccaacgcc aaggttgacc gcctactgga agaagcgcgg caaacttcag acctagaaga
     7681 gagaaacaag aaatattttg aagcagaaac agagataaaa aaagatcagc cggtcgcttt
     7741 tatttattcc ccttatttcc tttatctttt gcccagtaac attatgggag tctccctgcc
     7801 aaccctaacc aatccggccg aacgttttgt caacattcac aaatggtatg ttcgcactga
     7861 taaggtctgg aagatttttg ccaatctcta taaataaaaa ctattatcaa cttagcaatc
     7921 tggcaacctt tgtttgcctc aaacttaatt attaatttat gcctataaaa agaatccgag
     7981 tggatgcaag cggcaatatt caaacctttg tcgctgacaa aaagaccgaa aaagttggcc
     8041 ggccttccgg caaaatgggt ggtcaacgct ttagcccaaa taaaaaaaca accggtcgag
     8101 gccatagccc gcgaaaaact tcacccagca aaacccaaag cgaggataac aagcctaaac
     8161 aaaaaatccc gccagtcgcg cccggagtaa tcagaattat tcccttgggc ggggtggagg
     8221 aagttggcaa aaacatgacg gttgttgaat ttggcaacga cattgttgtg attgatattg
     8281 gtttaacttt tccgggcgaa gaagcgccgg gtgtggatta tattatcgcc gacccaacct
     8341 atctggaaga acaaaagtcc aaaatccgcg ccgttattat cacccacggc caccttgacc
     8401 atattggcgg tatcccctac gtgatgtcgc gaatcggcaa cccgccgatt tacacttctc
     8461 tcttgtcggc tgttatgata aaaaaacgcc aagaggaatt tccccatctt ccggctttaa
     8521 acataaatgt cgttagcgaa aaggataaaa taaaacttgg taatttatca atccgtttct
     8581 ttgccaccac ccacactatt ccagattcta tcggggtcat cattgaaaca cctttcggta
     8641 acgtgatttt taccggcgac ctaaaagtgg aaaacacaaa cggcgtgccg gaaaaatttg
     8701 aagaggaacg ttttaacaaa cttgggcaag aaaacaacct actgttagtg gctgactcca
     8761 ccaacgtgga aaaaccaggt ttttcttacc cggaaaaaga agtgcaaaaa aacgtcaagg
     8821 agattatcgc caacgccaag gggcgagtaa ttattggcac ttttgcctct ttgtttgacc
     8881 gcattattgc cattattgaa gcctgcgagg aattgggcaa aaaagtagcc attgagggcc
     8941 gcgccatgaa aaccaatatc gagattgcca aagagcttgg ctacttaaaa accaagcgtg
     9001 gcaccttcgc cactccggat gagatggcca ccttgcccga aaacaaaatt gtaattttgg
     9061 ccactggcgc ccaaggggac gaatacgccg ccttgatgcg tatctcccaa aaagaacaca
     9121 agtcggtcaa aatcaaaaaa ggtgatacga tcttgctttc gtcgtccgtt attccgggcc
     9181 acgaaaaagc tgtccaaaga ctaaaagata atttagcgcg ccaaggcgcc aagattgttc
     9241 attatggcat tgccaacgtc cactcgtcgg gccactccta tcaagatgaa attgcttggg
     9301 ttaataaaaa actcaaaccg cgttttcttt taccggttca tggtcaccag tttatgctcc
     9361 acgtccacgc taacgtcgcg cacgaaataa tcggtatgcc tgaaaaaaat attatcgttc
     9421 cagaaaacgg ctccattata gaaatctccg acaaaggaca gaaaatctcc gccctcaaac
     9481 aaatggcttc ggccaaactg gtaatgattg acggacttgg tgtagacaac gttcaagagg
     9541 ttgttattcg cgaccgccaa atgttggccc aagatggaat gttcgtcttg attgccatca
     9601 tagatatcaa aaccggcaag gttcgtaaat ctcccgacat tatctcgcgt ggctttgttt
     9661 acctcaaaga atcacaggac cttttgcgtt atgtccgcct cttgaccaaa aaaaccattg
     9721 aagactccac tgcccaaatg cacccgatta acctggacta tgtcaaaaat aacgtccgtg
     9781 aagagctcgg caaatatctg ttccaaaaaa cccacaagcg acccattatt ttaccagtgt
     9841 tgatagaagt gtaagattaa ctcaaggcta aataagtttt taacgctcaa tgtttcaagt
     9901 tttgagttaa ttttgttata atcacctcat gcgttcccaa aaaactctta ttctgttcac
     9961 ttatttgtgc ggtctaacca tgaccttttc ttttgctttc cctggttatg ttaactccac
    10021 ttttctggaa aacttcgttg gtgaaaaatg ggttggtttt atttttgcca tcggcgcctt
    10081 gctgtcggtt atcttgatgt cgcaaatatc caaactgatc aaggcctatg gcaatatcaa
    10141 agttttggtt tcttcggccg ctctcgctat ttttgttttg attttgcttg cccttgctaa
    10201 atctgttact gtcattattt caaccttcat catctactac tgtttggggt ttttcatccg
    10261 ctacacctta gatgtctacc tggagaatat ttctgatgac aaaaacaccg gtttaattag
    10321 gggcttttat ctgacatttt taaataccgc ctggctaatc tctccatttt tggcgagccg
    10381 cctggtggtt aatggcgatt tctggaaaat ttacgtggtt gcggcaatgt ccctcctacc
    10441 cctaatcgcc atcgccagcg tttttcttta tgaacaaaaa gacctcaaat atgacggcca
    10501 aagcatattc ccccaactca aacgtctatg gcgcggagag aaccaaactg accgctgtat
    10561 tcgccgtatt ttgatagttg attttttgct ctgtcttttt tacgccgtaa tggtcatcta
    10621 tatgcctctt tatttgcatg agcatcttgg tctcccttgg aacgaactcg gcttggtctt
    10681 tactatcatg ctggtcccct tcgttatcct ggagctgccc cttggccgca tcgccgaccg
    10741 ctggatgggc gaaaaggaaa ttttgatggg cggcttagtc attaccggat tatccacctc
    10801 ggtcttagct tttatccctt cagccagttg gttggtttgg gccggtcttt tgtttatcac
    10861 ccgtgttggc gcggccggca ttgaagtaat gaaggaagct tatttgttta agaaaataga
    10921 cggaagcgac actagtctca tttctttgtc gcgaatcatc gtgcctttct cctatctcgt
    10981 cggtccactt tttgtctctt tgattctttt gttttttgac tttaactatg ttttcttctt
    11041 cctgggtttg ttggttcttt caggcctctt tttcgccacc aagttaaaag acaccaaata
    11101 ggagccgggg atccgcgcgg cctgcgtcta gctcgcgtta caagttcatc aattggcaat
    11161 attcacactt agggtcatca caagtttccg tcaaaattcg gccggtccaa acactttcaa
    11221 gcaacgcccc aatttcgccc ttgacccgag aaatatcctc cgcggcaatg ggaatagaaa
    11281 ccgtcggaca ccgccccttg ttgtccggtt taacaaaaac caacgccggt tcaatcgtct
    11341 gcccgcgata aaaagagctc tcggctaaca aaattttata aaaaatcaac tgacggaaat
    11401 aattgccatc atcattctta gtttcgcctt tgatcgccgc ttcgctcatt gccgattttg
    11461 ttttgtaatc aaaaaccaga accttctccg cggtatcaat aatcgcgtcc atcttgccat
    11521 gaattttcag ttcaaccgcc tgtttttcta ctttgaactc aaacctggcc tccacccatt
    11581 tttccgccgc tgctacgcct tttatcgcaa aatgtggtcc aagcgcggcc gccactttgg
    11641 gcgcggccaa aagaagctct tccagagcca gttccttgtc tgctgacaac aaaagagaat
    11701 gcgcaaaata attttgtaag gccaccttta gagtctcagt aattttctcc tcggtttttt
    11761 ccgacaaagc ccaaaccgcc gcgattgctt cgtgcatcgc gttgcctttt tcagccggca
    11821 tcgccggcgc ttccggcacc ttcaaaatac ttttgaaaaa gaatttgctt ggacactcgc
    11881 aaaaatgatt caaagcggtc accgacaaac ctttttccag caaaatattt ttggcaaatt
    11941 ccactagccg cgcctcgtct tttttcgcca actgagacaa gttaaccgcc gccggagctg
    12001 tcaccaaagc cggcaggctg gtccgcgcca cattttcttg gtccaattcg tcaataaagc
    12061 gcaagggagt caaggttcgt ttcattccat ccgccaaacc gacaaccaca accgcgcgat
    12121 gcctggcccg cgttaaggcc acataaaaca accgcctcgc atcgcgtaaa tcatcatttt
    12181 cttccttgct ctctggcaat aaaaagtaat tgccaaaacc gcgcgacatc caagattctt
    12241 ctgtcgcgta aggcaagaaa acataatcaa actccaaccc tttggaccca tgcgcagtca
    12301 tgattcgcac tcgcgcttca ccaaaaccag ccataatttt gacactcttg ttttcggctg
    12361 tttgtcggaa agacaacatt gcctcaatca acaaaatcgg gtcttctatt ttttgcgtcc
    12421 gcgccaagtc ctcgcccagg gccacaattg accgccatac ctccatcgaa agcgggtcgc
    12481 caagcgtcaa agccaccagt ccggattctt caccgactaa aattaaaaat tccaccgcgc
    12541 taacggaaac cgccttttcc ttgagaactg aaagttttga taatttttta tccaaatctt
    12601 tcagatttcc acttctgata tttttcatta cttccgcccg ttcgaacaaa tcaagacccc
    12661 acagcccgcc agataaagca taggccaaag attctgtttt agcggcatcg gccaaatatt
    12721 cggccaacgc aaaaaacagg cgtccaagcg gatgaccaaa cacgtcagcg ccgcgttccg
    12781 ccgccgccgg cacgccggcc gtttccaaga cccgcagagc attttccacg tcacgattgg
    12841 ttttcgtaat aaccgccaca gtcgctttgt cgtcggccat caacactttt tttatctcgt
    12901 cggccaaata tttttccgct gccgccacgt tgccggccat taccacctct accggttccg
    12961 gttcttcgcc gttcgcggac ttcaatctga tacgcaactc ggggtactca tccgcctggt
    13021 aattgttttc aatcaaacca aaacaagcat ccaagattcc ctgatgagaa cgataattgt
    13081 tttccaacga aataactttc atcgccggcc aaaggctttg aaaacgcaaa aaattttgca
    13141 ccgatgcgcc ctgaaaacga tagattgctt gtttttcatc gcccaccaca aacaagttcg
    13201 gctcttcata aaaatcggca atttttttaa tcagcgcgtt ttgcgcgtca ttggtatctt
    13261 gatgttcgtc aaccaaaata taaagatatt tttcttggag taaacgcaaa agcaattcat
    13321 cttgctccag tgcttgtgtt aattcaaaaa tgaggtcgtc aaaatcaatc cgccgctcaa
    13381 tttttttctt ttcttcgtag gtcgcgtaaa cttcggcaaa aattttagtt ttttcggctt
    13441 tatcaatttt tttcagagcg tcagccttaa gctggccctt ggtcgctccg cgactagaaa
    13501 ttgaagccgg gtcatttttg ataacttcaa tttgcgccgc cgcgaagtcg gcaatcattt
    13561 ccggcgtcca agcttctttt cggcagtcgc taatcgcttt gataatcttg cccacataaa
    13621 aatccgggtc acccagcggc cgcaggcgag aaaatctttt tgcgcgcaga atttcacgaa
    13681 taagagtttc tgcttccacc tcggtcagtt gtttggcttg acgtaagtgg ggaaaatggt
    13741 ctccaaattc cgccatcacc gccgacgcaa agccgtggta agtataaacg ccaatttcat
    13801 cagccgccga accgataaac tgccgcaact tgagacgcat tgctttttga ccggcttcag
    13861 taaaagtcag cgctaaaatg ccggacggtg gcgtatcagt ttgatacaaa atgttggcca
    13921 ctcgcaaagc caaaatttga gttttgccgg tgcccggtcc ggcaatcacc atcactgggc
    13981 cttcaatcgt gtccacggct tctttttggg ccgagttgag gcgattataa gcttcattaa
    14041 acctcgcttg gtattcttcc catttccggg ccatttgggc gttcatgccc aaattatagc
    14101 attcccaagc tttttctacc ccttgcatct ttttaataaa tatgctacta ttaaaaggta
    14161 gggagccggt ccgcattgta tcattatcat tatatacagg gccggctttt ttgttttgac
    14221 gtttttattt tgtgccgctc tcgcctcttt gatttttgtg ctaagataat aaaaagaaaa
    14281 caagcgaaat ttaaaaatta aagcataaac aaatcctaaa gttttttaaa aaagcaagat
    14341 taacattaag aaccatgtta caccaacaaa ttaaagacga aataaaaaag gctatgttag
    14401 cgcgagaaac cgagcgcctg atggccgcgc gcaacatttt ggccgctttt accaacgaac
    14461 tggttgtcaa aaaacagagg ccggatgaaa tgttaaccga cgacgccctt gcggttttga
    14521 agcgcctcgc caaacaacgc aaagattcca ttgagcaatt taccatc
//
LOCUS       DAOOLO010000015        14164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_4632_length_14164_cov_6.997307, whole genome shotgun sequence.
ACCESSION   DAOOLO010000015 DAOOLO010000000
VERSION     DAOOLO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14164
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4632_length_14164_cov_6.997307"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLH96_01545"
     CDS             complement(<1..868)
                     /locus_tag="PLH96_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB60487.1"
                     /translation="MDWAKNKKIILFSALSSLGVFLILIGILVIAFLTNQGGILDRLA
                     TSIMNRGNLPSPVLSQDWYVINAVKKASPSVVSIGIVRTVPIVERYFEQRIFGDFEVV
                     VPQIREKGTEKQEVGGGSGFIVSEDGYIVTNKHVVETPDVDYVVYTNNGQKYESKVVA
                     LDPIYDVAILKINGGNFSPIELGDSDKLQVGQTLIAIGNALGEFRDTVSTGIVSGLSR
                     SIFAAGGAGQLEFLDEVIQTDAAINLGNSGGPLLNLRGEVVGVNVATAIDSENIAFSL
                     PINIVKPIIESVK"
     gene            complement(908..1606)
                     /locus_tag="PLH96_01550"
     CDS             complement(908..1606)
                     /locus_tag="PLH96_01550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB60488.1"
                     /translation="MSYPMRINKYLANRGLTTRRGADELVRQGKVLINDQPAKLGDLV
                     QTSDKVEVKDSLAKKAESLVYFAYYKPRGEETDKLPPAKLPKGSPQNIFPVGRLDKDS
                     RGLLIMTNDGRVTDRLLGPAYKHEKEYEVEVDKVVNGFMLKRLEMGVNIEGYKTKKCQ
                     VSKIGPKNFRLTLTEGKKHQIRRMCAAVGLQVKDLKRIRIMNIELAKLKPGQLRPLVE
                     TEKTEFLKALSLPF"
     gene            complement(1676..2122)
                     /locus_tag="PLH96_01555"
     CDS             complement(1676..2122)
                     /locus_tag="PLH96_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HPB60489.1"
                     /translation="MITLLQKARGFSSWVWLWLAFGQVVVATLGSLFFSQILKLPPCI
                     LCIYQRGLMYPLLIILGVALWKKDERAHFYAFPFVILGAVVATYHNLIYWNLIPELTT
                     ACSINVPCLNDGYQIFGFISIPFLSLLAFIITGGFLIIFARKNKPE"
     gene            2207..2896
                     /locus_tag="PLH96_01560"
     CDS             2207..2896
                     /locus_tag="PLH96_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB60490.1"
                     /translation="MKKVAIFDVDGTIFRSSLLIELVEELIEAKIFPAGCQRGYKKEL
                     ERWLNREGSYEDYIDAVVKIFMKNIKGVYYGDFVEVGKAVIQRKQKRVYRFTRDLITQ
                     LKKKGYFLLAVSQSPKVVLDNFCDKLGFDKVYGRFYELGPGDKFTGRVVDEHLISNKA
                     NIVKRALEKEGLTLKESIAVGDTEDDIPMLETVEKPICFNPNQKLLRYAKLNNWRVAV
                     ERKDVIYEIKK"
     gene            complement(3042..3479)
                     /locus_tag="PLH96_01565"
     CDS             complement(3042..3479)
                     /locus_tag="PLH96_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60491.1"
                     /translation="MRWRFGFIKRNAFRKGIAKRVELDVSSDALLERNVDIRLFKMGY
                     FYANQEDPAFLVWLKFFPWHEVPLSNGGKLPPLALGYGDFGSSVQAPGDGLWRNKFAV
                     TPNHPLRFVAADNGHNLAGLFSSNQGFVEQGNYSSASFFVSRP"
     gene            complement(3559..4842)
                     /locus_tag="PLH96_01570"
     CDS             complement(3559..4842)
                     /locus_tag="PLH96_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60492.1"
                     /translation="MKSKIFNLLFLFLFLGGFWVGAVPAQAQQETNLYFFWGFGCPHC
                     AKEKEFLSELSAKYPELEIKRFEIYFSKKNQQLLEKIVEKLKIESLGVPLTIVGDEVF
                     VGFSEGVNDNLIQERVEECLVSGCPDETASLIEPPVLSSDPVSNSTFPAEEGVAEKEL
                     VTTTSTTSPVVVVASTSPLDNLKLPIFGSLNLSGYSLPALAVILGALDGFNPCAMWAL
                     LFLISLLLGMENRRRMWLLGSAFIITSAAVYFIFMAAWLNLIIFLGFVPWVKLLIGLV
                     AVTGGGYNLWEFFKNRSGTCKISESPNQAGTLKKLKRAVQERNLWLALGGIIALAFAV
                     NLVELVCSAGLPAVFTQVLVLNNLSSWRYYLYILLYIFFFMLDDLVVFFVSMVTLRLT
                     GVTTKYSKASRLVGGVLMVVIGLLLIFKPEWLLFG"
     gene            complement(4842..5120)
                     /locus_tag="PLH96_01575"
     CDS             complement(4842..5120)
                     /locus_tag="PLH96_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPB60493.1"
                     /translation="MPRPRLRRRVRFNPTAKYFKPQGIPLRLLDEVELSLEEMEAIRL
                     KDFVGLEQTEAAEKMKTSQSTFQRILAVARKKVADAFINGKAIRINEE"
     gene            complement(5195..5452)
                     /locus_tag="PLH96_01580"
     CDS             complement(5195..5452)
                     /locus_tag="PLH96_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPB60494.1"
                     /translation="MPRFDGTGPNGQGPMTGRGLGPCADGRPRVGWRRYFRGFGRRFG
                     LGGGFGRDYSNQPSMTKDEKKEWLQTEKEAIEEELKDLDNE"
     gene            complement(5552..7780)
                     /locus_tag="PLH96_01585"
     CDS             complement(5552..7780)
                     /locus_tag="PLH96_01585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB60495.1"
                     /translation="MEKIKQDKAWIVFLGTYPPRECGIATFTADLTNNFDELYSPREE
                     TKVVAINNNLQTYKYPRKVIAQISENDPDGYQAVAEKLNRLSQVKLVSVQHEFGIYGP
                     DYGKNLLIFLATINKPVVVTFHTVLPEPSSGQKEVVIKIVERADCLVVMTERAKKLLL
                     EVYSAPEEKIRVIHHGIHPLAYNDGSDLKKELRLSKRKIISTFGMLNKGKGIEYAIEA
                     MTEVVKQFPDVLYLVIGATHPVVLKQEGETYRNQLIEKVYSLGLQNNIRFYNKYLTTE
                     DLLKFLQATDIYLSLSQDPNQAVSGTLSYALGAGRPVISTPFPQAEEIITPDVGSLIK
                     FGTSEGIADQIISILGDPAKMASLGKTAYFKTRKMTWKNVALSYMREFIELLPELGKR
                     EKNLPAIKMRHLAKLTDKFGIFQFAILDEPDSEWGYTLDDNARALVVTSWYYDLTKNA
                     TAKKLAEVYLNFIKKAVDPKGGFKNYFKTKHLNQTEINNEQGLEDSAARAIWALAVAA
                     SGSLPVGLKKTAKQILESNLDFKKISSPRAMAFMIKGLSKYYRLVEKNKEEKEKIEGL
                     LTEYADKLLELFNHHSDENWLWFENYLTYSNAVLPEAMLWAYRVTGNYHYFKAGKQSL
                     DFLSENYFQGDVCVPVGQAGWYKRGEKKSLYDQQPEEVAVLVLALRAMYDLGEEEQYQ
                     AKMYKAFNWFLGNNILNRFMYSHLSGGCFDGLQEKEVNLNQGAESTISYLLARLALET
                     KA"
     gene            complement(7790..8821)
                     /locus_tag="PLH96_01590"
     CDS             complement(7790..8821)
                     /locus_tag="PLH96_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPB60496.1"
                     /translation="MFKLKKHSGGPVLSPAKNLDWEKEGVFNPGVIKDGEEIIMLYRA
                     VGERESYISRLGLAKSRDGINFERVSNDPVFGPGEFFDHWSTEDPRIVKIDNEFLITY
                     VAVADRIMKNGRSILRPLPLETASALLKTKDFSNFEKVGIISPAGSDNKDVVIFPRKI
                     RGRYAMLHRPNRWSKEWFSFSYEKFVDEGLPCPVERLPDQPSVWLAWSDNLKNWTDHK
                     LLIWPEHHLDEKVGAGLPPIETPDGWLLIYHHVSRNWNTGAFIYTARVALADLDNPNR
                     LIAKIPYDILAPDMPHETNGEIKVVFPTGGFVQGDVLHVYYGASDRYVCLATGSLSEL
                     LTELKKYKI"
     gene            complement(8830..9180)
                     /locus_tag="PLH96_01595"
     CDS             complement(8830..9180)
                     /locus_tag="PLH96_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPB60497.1"
                     /translation="MSNLDIYQEERPWGHFRRFTDNVSSTVKIISLKPDAELSLQSHQ
                     KRSEFWRVLAGSGFVQVGEGERPLSVGDEVEIPLGAKHQLSAGPDGLEVLEISLGEFA
                     EEDIVRYKDKYGRI"
     gene            complement(9241..10032)
                     /locus_tag="PLH96_01600"
     CDS             complement(9241..10032)
                     /locus_tag="PLH96_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPB60498.1"
                     /translation="MIDTSDKKIIVFDLDGTLAESKQVLGLEMAELLARLAGQKKVAV
                     ISGGSYRQFQKQFLPTWREILNERPAEEVQSLNENLFLLPTSGSQIYGYNQKMGDWQI
                     LAQEDFPAELKEKVKKVFETIVESADYDIPTESYGEQLEDRGTQITFSALGQDAPLGK
                     KKIWDPNQVKRQKIKKFLEQEIPEADVSIGGATSIDVLPKGFNKAVGLQKLLDLLFLE
                     KNDLIFVGDALFPGGNDYSAFVAGFETVKVENPLETSDLIGKFLS"
     gene            10442..10696
                     /locus_tag="PLH96_01605"
     CDS             10442..10696
                     /locus_tag="PLH96_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPB60499.1"
                     /translation="MFYVYILKSLKDDNIYFGSTNDLHRRLSDHNTGKVLSTKTRRPL
                     ELRYYEAYFKESEARHREASLKKDGRALAQLKRRITDSLQ"
     gene            complement(10710..10781)
                     /locus_tag="PLH96_01610"
     tRNA            complement(10710..10781)
                     /locus_tag="PLH96_01610"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10746..10748),aa:His,seq:gtg)
     gene            complement(10834..11385)
                     /locus_tag="PLH96_01615"
     CDS             complement(10834..11385)
                     /locus_tag="PLH96_01615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB60500.1"
                     /translation="MFQTGKGDDGKTRVFGCEQRLSKGSLVTETLGSLDEANSFLGWC
                     KTKAAKEKKFDKIADQNLVDLLAKAQNGLFSVQAELAGADKRVSAEQVAKMEEVIAAV
                     EKEIPPIESFVVPGGCELSAMFDFGRTIVRRTERMVVRAKERIPIQPESLAFMNRLSS
                     LLYALARLSNHRVGAGEEKPNYE"
     gene            complement(11436..11693)
                     /locus_tag="PLH96_01620"
     CDS             complement(11436..11693)
                     /locus_tag="PLH96_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60501.1"
                     /translation="MAGYVDFKRMGAPPIPVVQIEFVALAGGREVGSGYIPIPGELGF
                     ATHLAAEFAKTTAAKRFEKYPRSAKIEIYLRIFIFAQSFQN"
     gene            complement(12241..12324)
                     /locus_tag="PLH96_01625"
     tRNA            complement(12241..12324)
                     /locus_tag="PLH96_01625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12288..12290),aa:Leu,seq:gag)
     gene            complement(12422..12982)
                     /gene="rplS"
                     /locus_tag="PLH96_01630"
     CDS             complement(12422..12982)
                     /gene="rplS"
                     /locus_tag="PLH96_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPB60502.1"
                     /translation="MSQLQGFEISPVEVAKRQKLGLRPGYTVRVWQKVPEKDGKFRRQ
                     AFEGLVIACKHGTEPGATFTVRRVASGVGMEKIFPLYSPMIDQVEVLREVKVRRSKLY
                     YIRDKAAREIRRRMRRVITGLKARKDEDDELVEQVIEGIEIEPEVLESSEAETTEKTG
                     ETEKSVTEEEKTEEESETPTETKEEE"
     gene            complement(12996..13733)
                     /locus_tag="PLH96_01635"
     CDS             complement(12996..13733)
                     /locus_tag="PLH96_01635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB60503.1"
                     /translation="MNDNIKIIAEDENYLVVNKPAGLAVHGANFNQESTLADWLIDNY
                     PNLAQVGEPLKLPAGRLVLKPGLVHRLDKDTSGVMIIAKNQETFFFFKEQFQARQTQK
                     IYRAILAGELKLTKDDLAVIDLPIGRSKKDPRRRVASAKAFGPLREAVTEYKLLENLS
                     GFSYVEAYPKTGRTHQLRAHFKALNHPIVCDKLYTERPVCPTPLARQALHALALVINV
                     PRGGRMSFEAPIPADMASALENLRTSC"
     gene            13954..>14164
                     /locus_tag="PLH96_01640"
     CDS             13954..>14164
                     /locus_tag="PLH96_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPB60504.1"
                     /translation="MLKSKVDGSFYIGYTRDLRRRFKEHNDGENISTKHKKPFELIYY
                     EAYKSDRDAKYRESNLKKHAQALKAL"
BASE COUNT         4165 a         3466 c         2758 g         3775 t
ORIGIN      
        1 ttttgaccga ctcaataatt ggcttcacga tattaattgg caaagagaac gcgatatttt
       61 ctgaatcaat agcggtcgcc acattcaccc caactacttc cccgcgcaaa ttgagaagcg
      121 gtccacccga gttgcccaag ttgatggccg cgtccgtttg gattacctca tccaaaaact
      181 ctagttggcc ggcgccacca gcggcaaaaa tagaacgcga caaaccggac acgatcccgg
      241 tggaaaccgt gtcacgaaat tcccccagtg cgttaccaat cgcaataagt gtctgtccca
      301 cctgcaactt gtcggaatct cctaactcta tcggcgagaa attgccaccg ttaattttta
      361 aaatcgccac atcataaatc gggtcaagcg ccactacttt tgattcatat ttttgaccat
      421 tattggtgta aacaacataa tccacatccg gtgtttccac cacatgttta ttggtcacaa
      481 tgtaaccgtc ttctgagaca ataaaaccgg aaccgccacc cacttcttgt ttttcggtac
      541 ctttttcacg aatttgcggg acaaccactt caaaatcgcc aaaaatcctt tgctcaaagt
      601 atcgttccac aatcggtacg gttcgcacga ttccgataga aaccaccgag ggagaggctt
      661 tcttaacagc gtttattaca taccaatcct gactcaaaac tggactgggc aagttgcccc
      721 tgttcataat tgaggtcgcc aatcggtcca ggatgccgcc ctggttggtc aaaaaagcaa
      781 tcaccaaaat tccaatcaaa atcaaaaata ctccaagact agataaggca gaaaataaaa
      841 ttattttttt gtttttagcc caatccataa gatttaaatt aaagctacta agtgaagagg
      901 ccgggaatta aaatggcaat gaaagcgctt ttagaaattc agttttttcc gtttcgacaa
      961 gaggccgtaa ttgacccggt tttagttttg ccaattcgat attcataatc ctgattcgct
     1021 tcaaatcttt aacttgcaaa ccgacagcgg cgcacattcg acgaatctgg tgttttttgc
     1081 cctccgtcaa ggtcaaccga aaatttttgg gccctatttt tgacacttgg cattttttgg
     1141 tcttgtagcc ttctatgttt acacccatct ctagtcgctt gagcataaaa ccgttgacga
     1201 ctttatcaac ctcaacctca tactcttttt catgtttata ggccgggcca agcaaacggt
     1261 cggtaacgcg tccgtcgttg gtcataatca acaaacccct agaatcttta tcaagccggc
     1321 caaccgggaa aatgttttga ggcgagcctt taggcaattt ggccggcggc agcttatcgg
     1381 tttcttcgcc gcgcggctta taatacgcaa aataaacaag gctttcggct ttttttgcca
     1441 gtgaatcctt gacctctacc ttgtcactgg tttggaccaa gtcgccaagt ttggctggtt
     1501 gatcgttaat caacaccttt ccttgtcgca ccaattcatc ggccccacga cgagtggtga
     1561 gacctctatt ggccaaatat ttgtttatac gcatcggata actcattttt tagattgtat
     1621 ctggtatgca ataaattgta tatagtctga agaaccttgg gtggcacggg ggaggttatt
     1681 caggtttatt ttttctggca aaaattatca agaaaccgcc ggttattata aaagccaaaa
     1741 gagacaaaaa cgggattgaa ataaaaccaa agatttgata accatcattg agacaaggaa
     1801 cattgataga gcaagctgtg gtaagttcgg ggataagatt ccaataaata aggttatggt
     1861 aggtagcgac aaccgccccc agaattacaa aaggaaaggc ataaaaatgc gcgcgttcgt
     1921 cttttttcca aagagccacc cccaatatta tcagcaatgg atacatcaag cctcgctggt
     1981 agatacacaa aatacaaggc ggcaatttta aaatttgact aaaaaacaaa ctaccaagcg
     2041 tggccaccac cacctggcca aaagccaacc acaaccaaac ccaagacgaa aaaccccttg
     2101 ccttttgtaa aagtgttatc attgctttat tataacctaa ggtgggccga caactaaagt
     2161 ccggcttcat cattacaact atctctgttt aatttaaata aacgttatga aaaaagtggc
     2221 aatctttgat gttgatggca ccattttccg ctccagctta ttgattgagt tggtggaaga
     2281 gttgattgaa gccaaaattt tccccgccgg ttgccagcgc ggttacaaaa aagagttgga
     2341 aaggtggtta aaccgagaag gctcttatga agactatatt gacgcggttg tgaaaatttt
     2401 tatgaaaaac atcaagggtg tttattatgg cgatttcgtg gaagttggta aagccgtaat
     2461 tcaaagaaaa caaaaacgag tttaccgttt tacccgcgat ttgattactc aattaaaaaa
     2521 gaaaggatac tttttgttgg ctgtctcaca atcgcccaag gttgtgcttg ataatttttg
     2581 tgacaagctc ggttttgata aagtttacgg ccgattttac gaacttgggc caggcgacaa
     2641 atttaccggc cgagttgtag acgaacacct aatcagcaat aaggccaaca ttgtcaaacg
     2701 cgcgctggaa aaagaaggct taactttaaa agagtctatc gcggtgggcg atactgagga
     2761 tgatatccca atgttagaaa cagtggaaaa accgatttgt ttcaacccca accaaaaact
     2821 tttacgttac gccaaactaa acaattggcg cgtggcggtg gaaagaaaag acgttattta
     2881 cgaaatcaaa aagtaaaaaa cttgcccttc tcacaaaaaa tgcaataata caaacggaac
     2941 tgaacccaaa ctcacaagga ggaaaaatgg gaggatttat cggattaggc cgagcccctc
     3001 tccaacggaa gcgtaggagg agagttgttt tcgccagcag cctaagggcg gctgacgaaa
     3061 aaagacgcgg acgagtaatt gccctgctca acaaagccct ggttggagct gaacaaaccg
     3121 gcgaggttgt ggccgttatc agctgcaacg aaacgaaggg ggtgatttgg cgttacggcg
     3181 aatttatttc gccagagtcc atcgccgggt gcctgcacac tagagccgaa gtcgccatag
     3241 cccaaggcaa gtggcgggag ctttccccca ttcgaaaggg gtacctcgtg ccaagggaaa
     3301 aatttgagcc aaaccagaaa ggctgggtct tcttggttgg cataaaaata tcccatcttg
     3361 aagagacgga tgtctacgtt tctttccaag agagcatctg aggaaacatc caactcaacg
     3421 cgtttcgcaa tccccttgcg aaacgcgttt ctttttataa aaccaaaccg ccagcgcaac
     3481 cttttttcag ataaaagcca aaggctctca attatttact gttggaaatt aaacacaaag
     3541 ggtgccccca ttaactaact agccaaacaa aagccactcc ggcttaaaga tgagaagaag
     3601 accgataaca accatcaaga cacctcccac taagcgcgag gccttggaat attttgtggt
     3661 taccccagtc aaacgaagag ttaccatgga gacaaagaac accactaagt cgtctagcat
     3721 aaagaaaaag atataaagca ggatgtaaag atagtatcgc caagacgaaa gattatttaa
     3781 aaccaaaact tgcgtaaaga cggccggcag accagctgag caaaccagct ccaccaaatt
     3841 aaccgcaaaa gccaaggcaa tgatcccccc caaagccaac cacaaatttc tttcttgaac
     3901 ggctcttttt aattttttga gggtgccggc ctggtttgga ctttctgata tcttgcaagt
     3961 cccgctcctg tttttaaaaa attcccacaa attgtacccg ccgcccgtca ccgcgaccag
     4021 accaatcaaa agtttaaccc acggaacaaa gcccaagaaa attataaggt taagccaagc
     4081 cgccataaaa ataaaataaa ccgcggccga agttatgata aaagccgaac ccaaaagcca
     4141 catccgacga cgattctcca tccctaacag cagactaatt aaaaataata aagcccacat
     4201 cgcgcaaggg ttgaaaccgt ccaaggcgcc caaaattacc gctagcgcgg gaagagaata
     4261 accagagagg ttcagtgaac caaaaatcgg caacttcaaa ttatcaagtg gcgaagtaga
     4321 cgcgacaacc accactggcg atgtcgtaga tgtagtagtc accagttctt tctcggctac
     4381 cccctcttcg gccggaaaag tcgaattgga aactgggtca gatgacaaaa ctggcggctc
     4441 aattaacgaa gcggtttcat ccgggcaacc cgagaccaga cattcctcca ccctttcctg
     4501 aatcagatta tcattaaccc cctccgaaaa tccgacaaaa acttcgtcac caacaatagt
     4561 caatggcacc cccaaagact ctatctttaa cttctcaact attttttcca gcagttgctg
     4621 atttttttta ctaaaataaa tctcaaaacg tttaatctcc aactctggat atttggctga
     4681 gagttcgctt aaaaattctt tctctttggc gcaatgagga caaccaaaac cccaaaagaa
     4741 atagagattt gtctcctgtt gggcctgagc cggaacagcg ccaacccaaa aaccacctaa
     4801 aaacaaaaac aggaaaagta aattgaaaat ttttgacttc attattcttc gttaattctt
     4861 attgctttgc cattaataaa agcatcagcc actttttttc gagccaccgc caaaatacgc
     4921 tgaaaagtgc tctgcgaagt cttcatcttt tccgctgctt cggtttgttc aagtccgaca
     4981 aagtccttta gtctaattgc ttccatttct tcaagcgaaa gttccacttc atcaagcaaa
     5041 cgaagcggaa tgccttgcgg cttaaaatat ttggcggtcg ggttaaatct aacccgcctt
     5101 cttaatcttg gacgtggcat agttataatt atatattcaa aattattaaa cgcaagcaaa
     5161 aagcgcgaca ataggcgcgc tttttgccaa gcgattactc attgtccaaa tctttcaatt
     5221 cttcttcaat cgcctctttt tctgtttgga gccattcttt tttctcgtct ttagtcatag
     5281 aaggttggtt tgaatagtcg cggccgaaac cgccacccaa accaaatcga cgaccaaaac
     5341 ccctaaaata acgacgccaa ccaacacgtg gccttccatc agcacaagga cccagaccac
     5401 gaccagtcat cgggccttga ccattggggc cagttccgtc aaatctaggc ataattttgt
     5461 ctaagttatg agttagtaat cacgacctat tttgagtata tacccataac taatatttgt
     5521 caagtttttg gttaaataat taatgaaaaa cttaggcctt cgtttccagg gccaagcgcg
     5581 ccaaaagata agaaatagtt gattcggctc cctgattaag attaacctct ttttcttgca
     5641 aaccatcaaa acagccgccc gacagatgag aatacatgaa acgattcaaa atgttgttgc
     5701 ccaaaaacca attgaacgct ttgtacattt ttgcctgata ttgttcctcc tcgcccaaat
     5761 catacattgc ccgcaaagcc aggaccaaga ccgccacctc ctccggttgt tggtcataga
     5821 gacttttctt ttctcccctt ttataccaac cggcctgacc gactggcacg caaacatcgc
     5881 cctgaaaata attctctgac aaaaaatcca atgattgttt cccagccttg aaataatggt
     5941 aattgccggt gaccctatag gcccagagca tcgcttccgg caggaccgcg ttggaataag
     6001 tcagataatt ttcaaaccaa agccaatttt catcgctgtg gtgattaaac aattcaagca
     6061 atttgtcggc atattccgtc agaagcccct caattttttc cttttcctct ttgttttttt
     6121 caaccaaccg ataatatttg gaaaggcctt taatcataaa cgccatcgct ctgggcgaac
     6181 ttattttctt aaaatcaaga ttgctctcca atatttgttt agccgttttt ttgagaccaa
     6241 ccggtaaact gccgctggcc gccacggcca aggcccaaat ggcgcgagcc gcggaatctt
     6301 ccaaaccctg ctcgttattt atttcagtct ggtttaggtg tttggttttg aaatagtttt
     6361 taaaaccacc tttcgggtca accgcttttt taataaaatt taaataaacc tctgctaatt
     6421 ttttggcagt ggcgtttttc gttaaatcat agtaccaaga agtcacaact agcgcgcggg
     6481 cattgtcgtc tagagtatag ccccactcag aatctggctc atcaagaatg gcaaactgaa
     6541 aaatcccaaa tttgtcagtc aatttagcca agtgccgcat cttaatcgcc ggcagattct
     6601 tttctctttt accaagttcc ggcaacaact ctataaattc tctcatatag gacaaggcga
     6661 catttttcca agtcatcttt ctggttttga aataagcggt tttgcccaaa ctggccattt
     6721 tagccgggtc tcccagaata gaaattattt ggtcggcaat tccctcgcta gtcccaaatt
     6781 ttatcaaact acccacatca ggagttatga tttcttccgc ctgtggaaaa ggtgtagaaa
     6841 taacgggacg cccagcgccc aaggcgtagc tgagagtgcc agaaaccgct tgatttgggt
     6901 cctgagacaa agataagtag atatcggttg cttgcaaaaa ttttaacaag tcttctgttg
     6961 ttaaatactt gttataaaag cgaatattat tttgcaatcc caagctgtaa actttttcaa
     7021 ttaattgatt ccgataagtc tccccttctt gcttcaagac cactggatga gtggcgccaa
     7081 taactaaata aagaacgtcc gggaactgtt taacaacttc cgtcattgct tcaatggcat
     7141 attctatccc cttgcccttg ttgagcatcc caaaggtaga aattattttt cgtttagata
     7201 atcttaattc ttttttaagg tcactgccgt cattataggc cagggggtga atcccatggt
     7261 gaataaccct aatcttctct tcgggcgcgc tgtaaacctc aagcagcagc ttcttcgccc
     7321 tttcagtcat aacgaccaaa caatcagcgc gctcaacaat cttgataacc acctcttttt
     7381 gaccggaaga tggttctggc aaaaccgtgt ggaaagtcac gacgaccggt ttatttatag
     7441 ttgccaaaaa aatcaacaaa tttttcccat aatcaggacc ataaatgcca aattcatgct
     7501 ggacagagac cagcttaact tgagataacc tatttaattt ttcagccacc gcctgatatc
     7561 catctgggtc attttccgaa atttgagcaa taaccttgcg cgggtatttg tacgtctgca
     7621 aattattatt gattgccaca accttagttt cttctcgggg ggaataaagt tcgtcaaaat
     7681 tattggtcaa atcagcggta aaagtggcaa taccgcattc acgtggcggg taggttccca
     7741 aaaaaacaat ccaggcttta tcttgtttga ttttttccat cttttccttt taaattttat
     7801 atttctttaa ttccgtcaaa agttcggaca atgagccggt cgccaaacaa acataacggt
     7861 ccgaagcgcc ataataaaca tgtaaaacat ctccttggac aaaacccccg gtcggaaaaa
     7921 caacttttat ctcgccgttt gtctcatgcg gcatatcagg agccaaaata tcataaggga
     7981 ttttggcgat taaccgattt ggattatcca aatcggccag agccactcgc gcggtgtaaa
     8041 tgaaagcgcc ggtgttccaa tttcgcgata catgatgata aatcaggagc caaccatcag
     8101 gcgtttctat cggcggcaag cccgcgccaa ctttttcatc caggtgatgt tcgggccaaa
     8161 tcaaaagttt gtggtcagtc caatttttta agttatcaga ccaagccaac caaacgctgg
     8221 gctggtctgg caacctttcc accggacaag gcaagccttc atcaacaaat ttttcatagg
     8281 aaaaggagaa ccattctttg gaccaacggt ttgggcgatg cagcatggca taacggcccc
     8341 tgatttttcg ggggaaaata accacatctt tattatcaga accggctggc gaaataatac
     8401 ccactttttc aaaattggaa aaatctttag ttttcaatag agcggaggca gtctccaggg
     8461 gcaacggccg caaaatagac cgaccatttt tcattatccg atcggccact gcgacataag
     8521 taatcaaaaa ctcgttgtct atttttacaa tccgagggtc ttcggtagac caatggtcaa
     8581 aaaattcacc cggaccaaaa actgggtcgt tagaaacacg ctcaaagttt atgccatcgc
     8641 ggcttttggc taaacccaat ctggagatat aagattctct ttcaccaacc gcccgataaa
     8701 gcattatgat ctcttcgcca tcctttatca ccccaggatt aaataccccc tctttttccc
     8761 aatctagatt tttggccgga cttaaaaccg gaccaccact gtgttttttt aacttaaaca
     8821 tggtaacaat taaatcctgc cgtatttatc tttgtaacgc acgatgtcct cttcggcaaa
     8881 ttcaccgagc gaaatttcca gaacctctag gccgtcaggg ccggcggaaa gttgatgttt
     8941 ggcgcccaag ggaatttcta cttcatcacc gactgaaaga ggtctctccc cctcgcccac
     9001 ctgaacaaaa ccgctccccg ctaaaacccg ccaaaactcc gatcgttttt gatgactttg
     9061 caaacttaat tccgcatccg gcttgagcga aattattttt accgttgatg agacattgtc
     9121 ggtaaaacgc cgaaaatgcc cccagggccg ttcttcttga tatatatcaa ggttggacat
     9181 accgagattc taacaaaaaa aagctgagat agccagccaa agcttttgcc ttgggctatt
     9241 ttagcttaaa aattttccaa ttaaatcact agtttctagc ggattttcca ctttgactgt
     9301 ttcaaaacca gcaacaaagg ccgaataatc gtttccgccc ggaaaaagcg cgtcacccac
     9361 gaaaatcaag tcgttttttt ctaaaaagag caggtcaagc aatttttgca aaccaacagc
     9421 tttattaaaa cccttgggca agacatcgat ggaggtggcg ccgccaatag aaacgtccgc
     9481 ctctggaatt tcttgctcta aaaatttttt tattttttgc cgcttgacct gattcggatc
     9541 ccaaattttc ttttttccca atggcgcgtc ttggcctaaa gcggaaaagg taatttgcgt
     9601 gccacgatct tccagttgct cgccatagga ttcggtcgga atgtcgtaat cagcagactc
     9661 aacaatagtc tcgaaaactt ttttgacttt ttcttttaac tcggccggaa aatcttcttg
     9721 cgccaagatt tgccaatccc ccattttttg attatagccg taaatctgac tgccgctggt
     9781 tggcaataaa aatagatttt cgttcaaact ttgtacttct tccgctggcc gttcatttaa
     9841 aatctctcgc caagttggta aaaattgttt ttgaaactgc cgataagaac cgccggaaat
     9901 aacggccacc tttttctgcc cggccaatct tgccagtaac tccgccattt ctaaaccaag
     9961 gacttgtttg ctctccgcca aagtcccatc aagatcaaaa acaataattt ttttgtcaga
    10021 ggtatcaatc atgcacaaga ctattttctg ctaaaacgca aaataaaaaa acttaaaata
    10081 aaagctatca gaaaaaacaa cgcgccataa aaacacgggg tcaaaaacgg attcactggt
    10141 tcggcactga taacacaacc cgttggacaa ggttcgctat ttagccaagc gaccaattcc
    10201 caaacaaaat ttgcccaagc aaaaattgtt cccaataaaa gaaccacaaa ctgtgaaata
    10261 attaattttt tcatataaga atattataga actttttgtc ccaaattaaa aagcagccaa
    10321 gtccggcaac gtcttggttt agagaaaaca catcgcaaat aatccaatgg atgagaaact
    10381 cagttggggt gcctacaccc acaccaatcc ctttatcgac gcaaatcata tataatttat
    10441 aatgttctat gtttatattc ttaaaagttt gaaggacgat aatatctatt ttggctcaac
    10501 taatgatttg catcgcagac tgtctgacca taatactggc aaagttctgt caaccaaaac
    10561 gcgccgtcca cttgaactta gatattacga ggcttacttt aaagaaagtg aagctcgtca
    10621 tcgggaagca tcactcaaaa aagacggccg agctttggct caactcaaaa gacgcattac
    10681 agacagtctg caataaaggg attggcgtgg gtgcctatcc ggactcgaac cggaattaat
    10741 tcctccacag ggaactgtgt taaccattac actataggca ccattaaact aactccaaca
    10801 atttactatc aaccgacaat ttaatcaact tgattattcg taattgggtt tttcttcccc
    10861 tgctcccacc cgatgattgg acaaacgcgc aagcgcgtaa agcaaactag acaagcgatt
    10921 cataaaagcc aaggattccg gctggatagg tattcgctct ttggcgcgca caaccatacg
    10981 ttcggtccgt ctgacaattg tccgcccaaa atcaaacatg gcagacaatt cacaaccgcc
    11041 aggcacaaca aagctctcaa ttggcgggat ttctttttcc acggccgcga ttacttcttc
    11101 cattttggcg acttgctcgg ccgaaactct cttgtcggcg ccagctaatt ctgcttgaac
    11161 agagaataag ccattttgcg ctttagccaa caagtcaacc aaattttgat cggcaatttt
    11221 atcaaatttt ttctccttag cagcctttgt cttgcaccaa cccaaaaaag aattagcttc
    11281 gtccaaagaa cccaaggtct ctgtcaccag cgaacctttt gacaaccgct gctcacaacc
    11341 aaaaactcgc gtcttgccgt catccccttt gccagtctga aacataagat atttatagca
    11401 caaatttggc cataacgaaa gggcgccggg gcgccctaat tctggaagct ctgcgcgaag
    11461 atgaatatcc gcagatatat ctcaatcttc gccgaacgtg ggtatttctc aaatctttta
    11521 gcggctgttg ttttggcaaa ctccgccgcc agatgagtgg caaaaccgag ttcccccggt
    11581 atggggatat aaccactccc cacttcgcgc cctccggcca aggccacaaa ttcaatctga
    11641 accaccggaa tgggcggcgc ccccatcctt ttgaagtcaa cgtagcctgc catattagct
    11701 cctttccgga gtccaatggc tccttagcta agccacctat ctaaccaaag catagcattg
    11761 cttgcggtac agtcaaaccc aaaccttaaa tcccaatttg aattgttgga agagaaatcc
    11821 aaaaagaaaa gcgccgaacc gcgaggttgg cgcttaaaac ctgaattcct cctgtgccgg
    11881 gcgagccggg gcaggaggcc tctcctcagc aacgaggaga actcccttcc tggccaaact
    11941 ggccagggac tggaaggggt accgggcatg aagcacccgg ctaatggcct ccttcgagga
    12001 gaccgcccac tgagtgggct tcttagccgg cacccggagc ccggttcggg agtccatctc
    12061 gtaaacatcg aactcccgag aaggccttcg ccttctttca gccatagcca tacaatcacc
    12121 tcctttctat ctatcattat agcatactca actaaaaaat gcaagagctg aaatacaggg
    12181 cttaatcggt aaggcgtaaa aactttcccc ttttccaacc cccaacttcc atcgtcactg
    12241 tgcgcgggag aggacttgaa cctccacgtt cttacgaaca cacgcccctc aagcgtgcct
    12301 gtctaccagt ttcagcaccc gcgcgttttc acggaaacaa gtatattatg cccaatgcct
    12361 tgttaatttt caacttttta aaactccaac aaaaaaatcc cgcctcggcg ggattttttg
    12421 cttactcttc ttctttggtt tcagttggcg tttccgactc ttcttctgtt ttttcttctt
    12481 cggtgactga tttttcggtc tctcctgtct tttccgttgt ttcggcttcg gagctttcca
    12541 acacttccgg ctcaatttca attccctcta ttacctgttc tactaattcg tcatcctcat
    12601 cttttctagc cttcaaacca gtgataaccc gacgcatacg acgcctaatc tcacgggccg
    12661 ccttgtccct aatataataa agtttggaac gacgaacctt tacttcgcgt aacacttcta
    12721 cctgatcaat cattggtgaa tagagcggaa aaattttttc cattcccaca ccagaagcaa
    12781 cccgacgaac ggtaaaagtc gcacccggtt cggtgccgtg tttacaggca atcaccagcc
    12841 cttcaaaagc ctgacggcga aatttgccat ctttttccgg cactttctgc caaacacgaa
    12901 cagtgtaacc cggacgcaaa ccgagctttt gtcttttggc aacttctacc ggtgaaattt
    12961 caaatccctg taattgtgac ataacgatga gcagtttagc acgaggttct aagattttca
    13021 agcgcagaag ccatatcggc cggtatcggc gcttcaaaac tcatccgccc tcccctaggc
    13081 acatttataa caagcgccaa ggcgtgaagc gcctgacgcg ccaaaggggt cgggcaaacc
    13141 ggcctttcgg tgtatagctt atcgcaaaca attgggtggt tgagagcctt aaagtgcgcc
    13201 cgtaactgat gtgttcggcc agttttcggg taggcttcca cataagaaaa accggacaag
    13261 ttttccaaca acttatattc ggtaacggct tcccgcaaag ggccaaaggc tttggcactc
    13321 gcgactctgc gtcgtgggtc ttttttgctc cggccaatcg gcaaatcaat caccgccaag
    13381 tcatcctttg tcaattttaa ctcccctgcc aaaatcgccc gataaatttt ttgcgtctga
    13441 cgcgcttgga actgttcttt aaaaaagaaa aaagtttctt ggttcttggc aataatcatc
    13501 accccgctgg tatctttgtc taaacgatga accaacccgg gcttcaacac caatcggcca
    13561 gccggcagtt tgagcggctc ccccacctgg gccaggttgg gataattatc aatcagccaa
    13621 tcagccaaag ttgattcttg gttaaagttg gcaccatgaa ccgccaagcc ggccggcttg
    13681 tttacaacca gataattttc gtcttccgcg attattttta tattgtcatt cattgcttat
    13741 aacttgataa tatagtataa attaatagca gtaaaaagaa aggcgcaaag aacgttaaac
    13801 aaatcccaat actcgcggtc ggccaaagag caatgcccaa gccgactgaa gccggaggag
    13861 aaagaggagt cgggagatta agtctgtaag ttgaaaatta acaagggcgc ttagcccctc
    13921 acccagaata gtcttgttat attctttgtt tatatgttaa aaagtaaggt agatggaagt
    13981 ttttatatag gttatactag ggaccttaga cgacgtttca aagaacataa cgatggggaa
    14041 aatatatcaa ctaaacataa gaaaccgttt gagttgatat attatgaagc ttacaaaagc
    14101 gatcgtgacg caaagtatag agaaagtaat ttgaaaaaac atgctcaggc gttaaaagcg
    14161 ctga
//
LOCUS       DAOOLO010000016        13694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_4901_length_13694_cov_6.194076, whole genome shotgun sequence.
ACCESSION   DAOOLO010000016 DAOOLO010000000
VERSION     DAOOLO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4901_length_13694_cov_6.194076"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="tuf"
                     /locus_tag="PLH96_01645"
     CDS             <1..952
                     /gene="tuf"
                     /locus_tag="PLH96_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPB60505.1"
                     /translation="APGHADYIKNMITGAAQMDGAVLVVAATDGAMPQTREHVLLARQ
                     VGVPKMIVFLNKCDMVDDPEMIDMVEEEIRELLTKQNFDGVNTPVIRGSGLKALEAKS
                     LDDEWAKKIMELIQAMDDFIPDPVREVDKPFLMPIEDIFSIEGRGTVVTGRIERGRVK
                     VGEEIEIVGVKDTMKTTVTGIEMFNKQLDEGMAGDNAGILLRGLKKEDVTRGQVLAKT
                     GSITPHTEFTAEVYVLTKEEGGRHTPFFNGYKPQFYVRTTDVTGEVTLAEDIKMVMPG
                     DTVNMTCKLINPVAMEEQMRFAIREGGRTVGAGVVSKIIK"
     gene            958..1320
                     /gene="rpsJ"
                     /locus_tag="PLH96_01650"
     CDS             958..1320
                     /gene="rpsJ"
                     /locus_tag="PLH96_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPB60506.1"
                     /translation="MPTAAKKTKKSSAKNEAGDIQKLRIKVSAYEYKVLDASVKQIVD
                     TAIRYDGQVLGPIPLPTEIKKYTVNRSSFIDKNSREQFEMRVHKRLIDILNPSPKIVE
                     SLTNLNLPSGVNIDVKMI"
     gene            1513..2112
                     /gene="rplC"
                     /locus_tag="PLH96_01655"
     CDS             1513..2112
                     /gene="rplC"
                     /locus_tag="PLH96_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPB60507.1"
                     /translation="MKFMLGKKEYMTQIFDESGKVVPGTVISAGPLTVAGHKNGRVVV
                     GYGERKLKNIAKPQHKLGEALGKEGVGFATIKELAVEETYNLGDKIDVSIFAEGDIVT
                     VSAISKSKGFQGVVKRHGFSGGRRTHGQKHSEREPGAIGAGGLQRVLKGLRMAGRMGG
                     ERVTVKNLTVAKVNKGENLIFIKGAIPGRKGTLVEIKGV"
     gene            2144..2806
                     /gene="rplD"
                     /locus_tag="PLH96_01660"
     CDS             2144..2806
                     /gene="rplD"
                     /locus_tag="PLH96_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB60508.1"
                     /translation="MEAKIYNQKGEEKGKLDLPEKFFGLPWNGDLVHQVMTSMLSNSR
                     SPIAHTKDRSEVRGGGKKPWRQKGTGRARHGSSRSPIWVGGGVTHGPRSDRNFSRKIN
                     KKMKAKAFFTVLSQKLRDGEILFAEAVNFDKPRTKDAQTYLSGLAKIKGYEKLVYKTG
                     KRAILALPEKDENLEKSFRNIKSASLMEVSNINPLDLLNYKYLVVLNPEKSLEALSAR
                     AK"
     gene            2813..3115
                     /locus_tag="PLH96_01665"
     CDS             2813..3115
                     /locus_tag="PLH96_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPB60509.1"
                     /translation="MSTIGKNLKTKIILRSPRVTEKSSVLAEGNRAVYVFEISQEATK
                     DEVRKAFQAKYNVKPEKVNVVNLPDKVTRRRGRAGIKQGVKKAMVFLKPGDKIEIV"
     gene            3136..3975
                     /gene="rplB"
                     /locus_tag="PLH96_01670"
     CDS             3136..3975
                     /gene="rplB"
                     /locus_tag="PLH96_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPB60510.1"
                     /translation="MKKYAPTTPSRRNTLTIEYKKRLSGDKPHKALTVGRKRSVGRNN
                     QGRITTRHHGGGNKRLYRLVDFKYDKKDIPAKVETIEYDPNRSAFIALVCYRDGERRY
                     HVVPKELKVGATFLVSDKADVKTGSRLPLYKISVGSFVYNVEVKPGSGAKLIRSAGSY
                     GEILSHDGGYTMIKMPSSEVRKIDDHAWASVGMVSNDEHKLTRKGKAGRSRWLGIRPT
                     VRGSVMNPVDHPYGGGEGRQGRGTRRPKTKWGKITGGRKTRPPKKYSNHLVVSRRKVG
                     KKR"
     gene            complement(3972..4118)
                     /locus_tag="PLH96_01675"
     CDS             complement(3972..4118)
                     /locus_tag="PLH96_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60511.1"
                     /translation="MSEHKFDTPLKQKAEIIYNLGSKLAAESDKKTSRRVVPTGFLVF
                     FFDF"
     gene            4321..4662
                     /gene="rpsS"
                     /locus_tag="PLH96_01680"
     CDS             4321..4662
                     /gene="rpsS"
                     /locus_tag="PLH96_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPB60512.1"
                     /translation="MSRSLKKGPYVDEKLLKKITGKKPAEAGVVKTWARRSQISPEMV
                     GFTFGVHNGKSHVEVFVTEEMVGHRLGEFSPTRKFVRHGGKMQKELEQKKKEAEIAAA
                     KAAKETTSAKK"
     gene            4710..5084
                     /gene="rplV"
                     /locus_tag="PLH96_01685"
     CDS             4710..5084
                     /gene="rplV"
                     /locus_tag="PLH96_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPB60513.1"
                     /translation="MKAFLKNYRQSPRKVRLVADLVKGKGLPQALAELDFLSKRAGDP
                     IKKLIKSAAANAKINFGTSEENLFIKSITVDKGHVMKRFQPASRGRAHPIRKKMSNVA
                     VTLESREPKQKLPKVAKKTTKK"
     gene            5121..5786
                     /gene="rpsC"
                     /locus_tag="PLH96_01690"
     CDS             5121..5786
                     /gene="rpsC"
                     /locus_tag="PLH96_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPB60514.1"
                     /translation="MTHSVHPYSHRIGIIRDWKSRWFGVGKNYREFLRADVCLREYLE
                     KRLRGFYVADLQIERGVNQYKIIIKTSRPGMLIGRGGDGVVKLKNDIYAKLKKIGAKI
                     PKELSVEIEEVKEPEAEAAIVAYMIAEGLEKRMPFRRVLKQTVEKVLSNKNVKGIKVV
                     ISGRLGGADMSRTEMIKRGQVPLQTFRADVDFAREKAYMYYGVIGIKVWIYRGEIFNK
                     PNE"
     gene            5818..6240
                     /gene="rplP"
                     /locus_tag="PLH96_01695"
     CDS             5818..6240
                     /gene="rplP"
                     /locus_tag="PLH96_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPB60515.1"
                     /translation="MPLLPKKVKYRKWQTGRANPKKLMRSDTRGIELAFGSFGLKAET
                     AGRIRSNQIEAARKTIARQMQKTGKLWIRIFPDRPFTAKAAEVGMGKGKGDPQGYEFE
                     VLPGRLIFELDGLSEEVAKEALRKAGAKLPVKTRVVKR"
     gene            6281..6478
                     /gene="rpmC"
                     /locus_tag="PLH96_01700"
     CDS             6281..6478
                     /gene="rpmC"
                     /locus_tag="PLH96_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPB60516.1"
                     /translation="MKKIDLKNKKEDELKKLLADKRKELSAFKFGISGSRTKDVKAGR
                     TTRREIARILTALHSKADMAE"
     gene            6493..6750
                     /gene="rpsQ"
                     /locus_tag="PLH96_01705"
     CDS             6493..6750
                     /gene="rpsQ"
                     /locus_tag="PLH96_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007233017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPB60517.1"
                     /translation="MKKNEKKVNKKTLKGEVVSDKTDKTRTVLVKRYVKHPLYGKYVT
                     ISKKYKVHDPENRAKVGQTVLISETRPISKDKHFVIVEVRR"
     gene            6828..7199
                     /gene="rplN"
                     /locus_tag="PLH96_01710"
     CDS             6828..7199
                     /gene="rplN"
                     /locus_tag="PLH96_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPB60518.1"
                     /translation="MIQPMSIVKIADNTGAKVGRVFKVLGGSKKRYARIGDLVVLSVQ
                     TAEPRKQVKKKDVLQAVVVRQRSPFRRDDGSYLRFDENAVVILEKGKKEPRGGRIFGP
                     IPREISEAGYQKIASLAPEVV"
     gene            7237..7542
                     /gene="rplX"
                     /locus_tag="PLH96_01715"
     CDS             7237..7542
                     /gene="rplX"
                     /locus_tag="PLH96_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPB60519.1"
                     /translation="MKLKKNDNVIVIAGKDKGKKGKIVRIFPVDGRVIVEGLNLRKKR
                     QRPKKQGEKGQTLEMAHPLEVSNVQLFCASCGKGVRVGAKVGGKKKIRICRGCGKEI"
     gene            7545..8078
                     /gene="rplE"
                     /locus_tag="PLH96_01720"
     CDS             7545..8078
                     /gene="rplE"
                     /locus_tag="PLH96_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPB60520.1"
                     /translation="MQTLKGKIEKSLPELKKSLKQDNDLAVPRLVKVVINTGTGRARD
                     KKRDELIVDRLAKITGQKASARGAKKSIASFKVRQGEVVGHSVTLRGQKMYDFLNMLL
                     NIAMPRIRDFKGYSEKSLDNMGNLTIGIREHVIFPQTADEEARDIFGFSVTIQTTAKN
                     KAEALAFFRAIGFPFKK"
     gene            8195..8704
                     /locus_tag="PLH96_01725"
     CDS             8195..8704
                     /locus_tag="PLH96_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB60521.1"
                     /translation="MIAIISDSHDNLANLEEFLKQAKKYKVGTIVHCGDVTTPECLEW
                     LVENFNGVIKVVWGNMEIRPEEFKNIEGKHHRLEIFDEIGLWETKSKIGKLKIAFSHK
                     PEKAERLAETGRYSYVFYGHTHKPWLSTTEEGVILANPGTIGGVFSEPTYALLDSTTG
                     KLELKKLFV"
     gene            8799..8984
                     /locus_tag="PLH96_01730"
     CDS             8799..8984
                     /locus_tag="PLH96_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPB60522.1"
                     /translation="MAKKSVIARSKREPKFASRVVRRCFRCGRKKAYMRDFGLCRICF
                     RELANDGKIPGVRKSSW"
     gene            8994..9386
                     /gene="rpsH"
                     /locus_tag="PLH96_01735"
     CDS             8994..9386
                     /gene="rpsH"
                     /locus_tag="PLH96_01735"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPB60523.1"
                     /translation="MITDPISDFLIQLKNASRVGKESVVVPSSKLKLEIANLLEREGY
                     LKNVAQKGKKVKKYLYCEIVYNGKEAKLKDVSRVSKPSRRIYVKTAELKPVRQGYGLA
                     VVSTPRGVMTEKEAKQQKVGGEHLFNIW"
     gene            9431..9970
                     /gene="rplF"
                     /locus_tag="PLH96_01740"
     CDS             9431..9970
                     /gene="rplF"
                     /locus_tag="PLH96_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPB60524.1"
                     /translation="MSRIAKQPIDLPQGTNAKLEKGAVLVTGPLGSLSRRVHSDVSIS
                     LLADNKVKVEPAHRSISADALSGTFASHVKNMIEGVTKGYEKKLLIEGVGFRFAVNGN
                     NVKLDLGFSHPVMLSIPEGLKVVVEKNLMTISGIDKELVGEFAAKIRALKKTEPYKGK
                     GIKYIDEVPQRKQGKKATA"
     gene            9985..10362
                     /gene="rplR"
                     /locus_tag="PLH96_01745"
     CDS             9985..10362
                     /gene="rplR"
                     /locus_tag="PLH96_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPB60525.1"
                     /translation="MRTIRIKTKEEKRKRRQARIRTKISGSAVCPRLAVFKSNKHMFV
                     QLIDDVAGKTLVSASDQEIKTEKGKKVDFAKVETSKAIGRLIAEKALAQKIKEVKFDR
                     GGNRFMGRIKAIADGAREGGLKF"
     gene            10377..10904
                     /locus_tag="PLH96_01750"
     CDS             10377..10904
                     /locus_tag="PLH96_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPB60526.1"
                     /translation="METTKTTTGNKKVFAPRGRRPGRRFGEREKPEFDQKVINVRRVA
                     RVMAGGRRFSFSVVVVAGDRNGRVGVGIGKAGDTSQAIEKAYNKAKKQMIKVALTSNK
                     SIPHEVEAKFKSARISLRPAPGRGLVAGSSVRIVLGLGGVKDVNAKIVSRSKDKLNIA
                     QATIKALQEVSKGKK"
     gene            10913..11344
                     /locus_tag="PLH96_01755"
     CDS             10913..11344
                     /locus_tag="PLH96_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HPB60527.1"
                     /translation="MQIQDVKRNTPQKKSRQIGRGGKRGTTAGRGTKGQKARAGNKIR
                     PNLRDQIKKIPKLRGYKFQSKNEKPTPVNLETIEKLFVDGASVNPASLAEKKIISRKG
                     GRFPQVKVLGGGTIAKKVSVSGCLVSAAAREKIEKAGGKIN"
     gene            11386..12660
                     /gene="secY"
                     /locus_tag="PLH96_01760"
     CDS             11386..12660
                     /gene="secY"
                     /locus_tag="PLH96_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPB60528.1"
                     /translation="MFATFLNKFNLILSERNLRNRILFVVAALAVFRLGASIPIPGVE
                     VFQLERFLAENQFLGLLNVFSGGGLSTLSIFMLGVGPYITASIIMQLLTFVFPSLKQL
                     FHEEGEAGRRKVAQYSRLLTVPLAVIQGLSLLVLLERQGVLMNLTMFDRVTNIFVVVA
                     GSLLVTWIGELITEFGIGNGVSLLIFAGIVSRFLPTLMQLWLTFDIAQAPMYLVFALI
                     ALIIVAAVVFVTEAERPIPVTYAKRVRGMKMYGGMSTYLPIRVNNAGVMPIIFALSIL
                     LFPQMIFGFLAGYDNEFISNVASFINSLLNNNWLYAGLYFLLVFAFTYFYSAVTFDPE
                     AISTNLQKNGAFIPGVRPGRSTAEYLSKILTRLTLIGAIFLGFVAVLPILLRSLTGIA
                     SLSIGGTSLLIVVSVALEFVKQVDAQVSMREY"
     gene            12827..13453
                     /locus_tag="PLH96_01765"
     CDS             12827..13453
                     /locus_tag="PLH96_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPB60529.1"
                     /translation="MNTLKTFIFIGPSGCGKGTQVELLIKHLTQTGDISTERPVLHSE
                     SGKRFRDFVKQADFMAGKTKLAMERGERLPDALAVWNWTGYLIEHYTGREHLIFDGSP
                     RSLLEAKALDTILSFLKRERAVVIFLDVSAEESIKRLLKRATEQGRADDDLEEIKRRL
                     DWFEKDVRPALEYYEKESTHLFLRINGEQSREKIHEDIKARLAEATKA"
     gene            13453..>13694
                     /locus_tag="PLH96_01770"
     CDS             13453..>13694
                     /locus_tag="PLH96_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB60530.1"
                     /translation="MITLKTQEEIEILKEGGRRLAVILRQVAELVKPGTETVILDDLA
                     LGLIRQGGDDPAFLNYRPSGSRLAYPASLCVSVNEE"
BASE COUNT         3896 a         2674 c         3487 g         3637 t
ORIGIN      
        1 cgcccctggt cacgccgatt atatcaaaaa catgattact ggtgccgctc aaatggacgg
       61 cgcggtgttg gtggttgccg ctaccgatgg cgcgatgcct cagaccagag aacacgttct
      121 gttggctcgc caggttgggg tgccgaagat gattgttttc ttgaacaagt gcgatatggt
      181 tgatgacccg gaaatgattg atatggtgga agaagaaatt cgcgagcttt tgaccaaaca
      241 aaactttgat ggcgtcaata ccccggttat ccgcggctcc ggcctcaagg ctctagaagc
      301 caaaagtctt gacgatgaat gggccaagaa aattatggaa ttgattcagg cgatggatga
      361 ctttatccct gacccggttc gtgaagttga taaacctttc ttgatgccga ttgaagacat
      421 tttttctatt gaaggtcgtg ggactgtggt caccggtcgg attgaaagag gacgagtaaa
      481 agtcggcgag gagattgaaa tcgtgggagt caaagacacc atgaaaacaa ccgttaccgg
      541 tattgaaatg ttcaacaaac agctagatga ggggatggcg ggcgacaacg ccggtattct
      601 cttgcgcggt ttgaagaagg aagatgttac ccgcggccag gttttggcca aaaccggttc
      661 catcacgcct cacactgaat tcacagccga agtgtatgtt ttgaccaagg aagaaggcgg
      721 tcgacacacc ccattcttca atggttacaa accacagttc tatgttcgta ctactgacgt
      781 gactggtgaa gtgactttgg cggaggatat caaaatggtt atgccgggtg acaccgtcaa
      841 tatgacttgc aaattgatta atccggtcgc gatggaagag caaatgcgct ttgctattcg
      901 tgaaggcggc cgcacggtcg gcgccggtgt cgtttctaag atcatcaaat aaattcaatg
      961 ccaaccgccg ctaaaaaaac taaaaagtct tccgctaaaa atgaagctgg tgatatccag
     1021 aaacttcgta tcaaggtttc ggcctatgag tacaaagtgt tggatgccag cgtgaagcaa
     1081 attgtggata cggccattcg ttatgatggt caagttttgg ggccaattcc tttgccgacg
     1141 gaaatcaaga aatacaccgt taatcgcagt tctttcattg acaaaaactc gcgcgagcag
     1201 tttgagatga gagtccataa gcgtttgatt gatattctga atccgtcgcc gaagattgta
     1261 gaaagtttga ctaatctaaa tttgcccagt ggcgtgaaca ttgacgttaa aatgatataa
     1321 aagcgaaaaa tcgcaaaaaa ccgccccggg ccagttggtc cggggcggtt tttggtcaca
     1381 catcataccc aatatacaat atgcaatgcg tggcttgttg acattccggc tttatttgtt
     1441 aagatatatc aaagctcgga ggggctattt cagtccgagg ctgattggcc taggactgaa
     1501 ttttttggcg atatgaaatt tatgctcggc aaaaaagaat acatgaccca gatttttgat
     1561 gaatctggca aggtcgttcc tggaacggtt atttcggccg gtcctttgac ggtggctggc
     1621 cacaaaaatg gccgagtggt agtcggttac ggcgaacgta aactcaaaaa tatcgccaag
     1681 cctcagcaca aactgggcga agcgcttggc aaagaaggag ttggttttgc gacaattaag
     1741 gaattagcgg ttgaagaaac ttataacctc ggcgacaaaa ttgacgtttc tatttttgcc
     1801 gaaggtgaca tcgtcacggt tagcgccatt tccaagagca agggtttcca gggggtggtg
     1861 aaacgacatg gttttagtgg cgggcgccgc acccatggcc agaaacactc tgaacgcgag
     1921 ccgggagcga ttggggctgg tggtttgcaa cgagttttga aaggtttgcg gatggctggt
     1981 cggatgggcg gcgagcgggt aacagtcaaa aacttgaccg tggccaaggt gaacaaaggc
     2041 gagaatttga ttttcatcaa aggcgcgatt ccgggtcgca agggcacgtt ggttgaaata
     2101 aagggagtct gattttatat tttttatatt ttaaattaaa accgtggagg ctaaaattta
     2161 caatcaaaaa ggtgaagaga aaggcaagct tgacttgcca gagaaatttt ttggtttgcc
     2221 gtggaatggc gatttggtcc accaagtaat gacctccatg ctttctaata gtcgttcgcc
     2281 gattgctcat accaaggacc gtagtgaagt tcgtggtggc ggcaagaagc catggcgtca
     2341 gaaaggaacc ggccgtgcca gacatgggtc cagccgctca ccgatttggg ttggtggtgg
     2401 ggtgactcat ggtccgcgtt cagacaggaa ttttagccgt aaaatcaata aaaaaatgaa
     2461 agcaaaggct ttctttaccg ttttgtcgca aaagttgcgt gacggcgaaa ttttgtttgc
     2521 ggaagcggtt aattttgaca aacccagaac taaggacgct caaacctacc ttagcggtct
     2581 ggccaagatt aagggttatg aaaaattggt ttataaaacc ggcaaaagag caattttggc
     2641 tttgccggaa aaggatgaga atctggagaa gagttttcgt aatatcaaga gcgccagttt
     2701 gatggaagtt tcaaatatca atccattgga tttgctcaat tacaagtatt tggtcgtttt
     2761 aaatccggaa aaaagtttgg aagcgctatc tgccagagcc aaataaaata ttatgtcaac
     2821 aattggcaaa aatttaaaaa caaaaatcat tctgcgttcg ccgcgggtaa cggagaaatc
     2881 cagtgttttg gccgagggga accgcgctgt ttacgtgttt gaaatttctc aagaagcgac
     2941 gaaggatgag gtgaggaaag cttttcaagc taaatacaat gtcaagccgg agaaggtaaa
     3001 tgttgttaat ctgccggata aagttacgcg tcgtcgcggc cgcgccggta taaaacaggg
     3061 ggtaaagaaa gcgatggttt tcttaaagcc tggtgataag attgagattg tctaaattct
     3121 aaaaaagttt ttattatgaa aaaatacgca cctacaactc catcacgccg gaatacgtta
     3181 acgattgaat ataaaaaacg cttgtccggc gataagccgc acaaggccct gactgttggt
     3241 cgcaaaagaa gcgttggccg aaataatcag ggacggatta cgaccaggca ccatggcggc
     3301 ggcaataagc gtctatatcg gttggtggat tttaaatatg acaaaaaaga cattccggcc
     3361 aaagttgaaa cgattgaata cgatcctaat cgttcggctt tcatcgctct ggtttgttac
     3421 cgtgacggcg agcgccgtta tcatgtggta cccaaggagc ttaaagtcgg agccactttc
     3481 ttagtttcag acaaggccga cgtgaagact ggcagtcgtt tgcctttgta taagatttcg
     3541 gttgggtctt ttgtttacaa tgtggaagta aagccgggaa gcggagccaa attaattcgt
     3601 tcggctggtt cctatggcga gattctctct catgatggcg gctacacgat gatcaaaatg
     3661 ccttcttcgg aagtgcgcaa aattgatgac cacgcctggg cttcagtggg aatggtttct
     3721 aatgacgaac acaaattgac gcgtaaaggc aaggccggac gcagtcgttg gctgggaatc
     3781 agaccaacgg tccgcggttc cgtaatgaac ccagttgacc acccatatgg cggcggtgaa
     3841 ggccgtcagg ggcgaggcac caggcgaccc aagaccaagt ggggcaagat taccggaggc
     3901 cgaaagacca gacccccaaa gaagtactcc aatcacctgg ttgtttctcg tcgcaaggtt
     3961 ggcaaaaaac gctaaaaatc aaagaaaaac actaaaaaac cagtcggcac gacgcgccga
     4021 ctggtttttt tgtcactttc cgcggctaat tttgacccta gattgtaaat tatctcggct
     4081 ttttgtttca agggcgtgtc aaacttatgc tcgctcatgc ttttgcttta gctttttgcc
     4141 caaatcgcgc ctctacgttc tccacccaag gcacaacata aaatatgaga cacgcaattt
     4201 gttgacattc cggctttatt tgttaagata tatcaaagcc acctaaggct ctttcgtggc
     4261 ccaaggctct tgctgttggg cggaatttag ctggtgaagt aggtggtttt taattaaaaa
     4321 atgtctaggt cgctaaaaaa aggtccctat gtggacgaaa agttgctgaa aaaaatcact
     4381 ggcaaaaaac cggctgaagc tggcgtggtc aaaacttggg ctcgtcgcag tcaaatcagc
     4441 ccggaaatgg tgggcttcac ttttggtgtc cacaacggta agagccatgt agaagttttt
     4501 gtgaccgaag aaatggtcgg acatcgtttg ggggaatttt cgccaactcg gaagtttgtg
     4561 cgtcatggtg gcaagatgca gaaggaactg gagcaaaaga agaaagaggc tgaaattgct
     4621 gccgctaaag ctgccaaaga aactacttct gccaaaaaat aatctgttta gaccaaacaa
     4681 cgaacaaact tttaaattaa aaactgacca tgaaggcttt tcttaaaaat tacagacaat
     4741 ctccgcgcaa agttcgcttg gtggctgact tggtgaaagg caaaggactg ccgcaagctt
     4801 tggcagagct agactttttg agcaaacgcg ctggcgaccc cataaaaaag ttaatcaaat
     4861 cggcggcggc taatgccaaa atcaattttg ggacaagcga agagaatttg tttatcaaaa
     4921 gtattaccgt tgataagggc catgtgatga aacgattcca accggcctcg cgcggtcgcg
     4981 ctcaccctat tcgcaaaaag atgagcaatg tggcagtaac ccttgaatca agagaaccaa
     5041 aacaaaagtt gccaaaagtc gcgaaaaaaa ctaccaaaaa ataattttat tatttcaatt
     5101 ttacattttt aattaacact atgactcatt ccgtccaccc atattcccat cggatcggca
     5161 tcatcagaga ctggaagtct cgctggtttg gggtgggtaa aaattatcgc gaatttctgc
     5221 gggcggatgt ttgtttaaga gaatatttgg aaaaacgttt gcgcgggttt tatgtggctg
     5281 acttgcaaat tgaacgcggg gttaatcaat acaaaataat catcaagact tctcgcccgg
     5341 gcatgttgat tggccgcggc ggcgatgggg tggtcaagct caagaatgac atttacgcca
     5401 aactcaaaaa aatcggcgcg aagattccga aggagttaag tgtggaaatt gaagaggtca
     5461 aagaaccgga agcggaagcc gcaattgtgg cctatatgat tgctgaaggt ttagagaagc
     5521 gaatgccttt tcgccgagtt ctaaaacaaa cagttgaaaa agttttgtct aacaaaaatg
     5581 tcaaaggtat caaggtggta atctccggtc gcctgggggg cgctgatatg agccgaacag
     5641 aaatgattaa aagaggacag gttcccttgc agacttttcg agcagacgtt gattttgccc
     5701 gcgaaaaagc ctacatgtat tacggcgtga tcggtattaa ggtctggatt tatcgtggcg
     5761 aaatctttaa caaaccaaac gagtaagttt attgagttta cattttaatt ttgcattatg
     5821 cctttattac ccaaaaaagt taaatatcga aaatggcaaa ccggacgcgc caatcccaag
     5881 aaacttatgc gttctgacac gagggggatt gaattggcct ttggttcttt cgggttgaag
     5941 gctgagacag cgggccggat tcggtcaaac cagattgagg cggctcgtaa aacgattgcc
     6001 cgccagatgc aaaaaaccgg taaactctgg attcgtattt tccccgacag accttttacg
     6061 gccaaagcgg cagaagttgg catgggtaaa ggcaaaggcg acccccaagg ctatgagttt
     6121 gaggtcttgc ccggccgttt gatttttgaa ttggacggat tgtctgaaga ggttgccaag
     6181 gaggctttgc gcaaggctgg cgccaaatta ccagttaaga ccagggttgt taagcgctaa
     6241 aacgcgtacc aatatctgaa ttcgggcaat tgaaacgatt atgaaaaaaa ttgatttaaa
     6301 aaacaaaaaa gaagatgaat taaaaaagtt gttggcagac aaacgcaaag aactttcggc
     6361 ttttaagttt ggaatttccg gcagccggac caaggacgta aaggctggac gaacgacccg
     6421 tcgtgagatt gccagaatct tgaccgcttt acatagtaag gctgatatgg cagaatagaa
     6481 tctgcgaaat ttatgaaaaa gaacgagaaa aaagtgaata agaaaactct aaagggagaa
     6541 gttgtttctg acaaaacaga caagaccagg accgttttgg ttaagcgtta tgtcaaacat
     6601 cccctttatg gcaaatacgt taccatttct aaaaaataca aagtgcatga cccggaaaat
     6661 cgcgccaagg taggtcagac cgtgttgatt agcgaaacaa ggccgatttc caaagataag
     6721 cactttgtga tagtagaagt tagaagatag gactaaagat aaaaaagagc agagcttaga
     6781 taaagcaaga taattttaca tttttatttt tacattttaa attacttatg attcagccaa
     6841 tgtccatcgt gaaaatcgca gataacaccg gcgcaaaagt cgggcgagtt tttaaggtgc
     6901 ttggcggttc aaaaaagcgc tacgcgcgaa ttggcgattt agtggtcttg tctgtccaaa
     6961 cagccgagcc gcgaaaacaa gttaagaaaa aggatgtttt gcaagctgtt gttgtcagac
     7021 aaagaagtcc ttttcgccgt gacgatggtt cgtacttgcg ttttgatgaa aacgcggttg
     7081 ttattttaga gaaaggcaag aaagagcctc gtggcggtcg tatttttggt cccatcccgc
     7141 gcgaaatttc cgaagccggg tatcagaaaa tcgcctcttt ggcgccggaa gttgtatagt
     7201 ggttgtgaac agtaatttgc aggtagcata tagattatga aacttaaaaa gaatgacaac
     7261 gtaatagtga tcgccggcaa agacaagggt aaaaagggta aaattgtccg catctttccc
     7321 gtggatggcc gagtaattgt tgagggtttg aatttgcgca aaaagcgtca acgccccaag
     7381 aaacaaggcg agaaaggtca aactttagaa atggctcatc cgctggaagt gtctaatgtt
     7441 caactttttt gcgccagttg tggtaagggc gtgcgcgttg gcgccaaggt tggcggcaag
     7501 aagaagatca ggatttgccg tggttgtgga aaagaaattt aatcatgcaa actttaaagg
     7561 gaaaaattga aaaatctttg ccggaactca agaagtcatt gaaacaagac aatgacctgg
     7621 ctgtcccgcg tttagtcaag gtggtgatta acactggtac cggccgagcc cgcgataaaa
     7681 agcgagatga attgattgtt gaccgtttgg ccaaaatcac cggccaaaaa gcatccgcgc
     7741 gtggtgctaa gaaatccatt gccagcttta aggtcaggca aggtgaggtt gtcggccatt
     7801 cggttacctt gcgtggccaa aaaatgtatg actttttgaa tatgcttttg aatatcgcga
     7861 tgccgcgcat cagagacttt aaaggttaca gcgaaaaatc tttggacaat atgggcaatc
     7921 ttacgattgg catcagggag catgttattt tcccgcaaac tgccgatgaa gaagcgcggg
     7981 acatctttgg tttttctgtt actattcaaa ctaccgctaa aaacaaggca gaagctctgg
     8041 ctttctttcg ggcaatcggc tttcccttta agaaataatt tctccttact ttcgtggggc
     8101 aattttttta agcgttttgc tccatttgct taatttattt tttaaaaacg cgaattaaag
     8161 ttttattttg ggcaccttgt tgaagtctca aaaaatgata gctattatct cagacagtca
     8221 tgacaatctc gctaatttgg aggagtttct aaaacaagcg aagaaatata aagttggcac
     8281 tattgttcac tgtggtgatg taactacccc ggaatgtttg gagtggttgg ttgaaaattt
     8341 taatggtgtg ataaaagtgg tttgggggaa tatggaaatt aggccggaag agtttaaaaa
     8401 tattgaggga aaacatcacc gcttggaaat ttttgatgaa attggtcttt gggaaacgaa
     8461 gagcaaaatt ggcaaactca aaatagcctt tagccataaa ccagaaaagg ctgaacgact
     8521 ggcagaaact gggcgttaca gctatgtttt ttatgggcat acgcacaaac cctggttatc
     8581 aacgacggag gagggcgtta ttctcgctaa ccctggcacc attggtgggg tcttttctga
     8641 accgacctat gctctcttgg attcaactac gggtaaatta gaactaaaaa agctgtttgt
     8701 ctaagcgttt gagagcgctt gccgccccgg ctttaattga caggggtgac tcttcttgtt
     8761 agtataaaga gtggctcgta gagccttatt agtttgctat ggccaaaaaa tcagtaattg
     8821 ccagatcaaa gagagaaccg aaatttgcct cccgtgtggt taggcgctgt tttcggtgcg
     8881 gtcgaaaaaa ggcatatatg cgggactttg gtctctgtcg tatttgcttc cgtgaattgg
     8941 ctaacgacgg gaaaattccc ggtgttcgta agtcatcttg gtaaatcaaa gctatgataa
     9001 ctgatccaat ttcagatttt ttaattcagc taaaaaacgc cagccgtgtt ggcaaagaat
     9061 cggtggtggt gcccagttcc aaattaaagc tagaaatcgc caacttgctt gaacgcgagg
     9121 gttacctgaa gaacgtggcg caaaaaggca aaaaggttaa aaaatattta tactgcgaaa
     9181 ttgtttacaa tggcaaagag gccaagctaa aagacgtgtc gcgcgtttca aaaccgtcac
     9241 gacgaattta tgttaagaca gcggaactca agccagttcg tcaaggttat ggtttggcgg
     9301 tagtttctac gccccggggt gtgatgaccg aaaaagaagc taaacaacaa aaggttggcg
     9361 gagaacatct gtttaatatt tggtagtttt ttgtttttaa attttaatct ttgtatttta
     9421 aattattatt atgtcgcgaa tagccaaaca accgatagac ttgccgcaag gcaccaacgc
     9481 caaactggag aaaggcgcgg ttttggtaac cggtccgctc ggctcgcttt cgcgacgtgt
     9541 gcattctgac gttagtattt cgctgttggc ggacaacaaa gttaaggtgg agccggctca
     9601 ccgttccatt tcggcagatg ctctttccgg cacttttgct tctcatgtca aaaatatgat
     9661 tgagggcgtg accaaaggtt atgagaagaa attgttaatt gaaggagttg gtttccgttt
     9721 tgcggtgaat ggcaacaatg ttaagttgga tttgggcttt tctcacccgg tgatgttgtc
     9781 catcccggaa ggtctaaaag tggtcgtgga aaaaaacctg atgacgattt cggggattga
     9841 taaagaattg gttggtgaat ttgccgctaa aatccgtgct ctcaaaaaga ccgaacctta
     9901 taagggtaaa ggcattaaat atattgacga agttccacaa cgcaaacaag gcaagaaggc
     9961 gacagcttag tttttgataa aagtatgaga acaatcagaa taaaaactaa agaagagaaa
    10021 cgaaagcgcc gtcaagcgcg aattagaacg aaaatttcag gttcggctgt ttgcccgcgg
    10081 ctggcggttt ttaagtctaa taaacacatg tttgttcaat tgattgatga tgttgccggc
    10141 aagaccttgg tctccgcgag cgaccaagag ataaaaactg aaaaaggcaa aaaagttgat
    10201 tttgccaaag tggaaacaag caaggcgatt ggtcggttaa ttgcggaaaa agccttggcc
    10261 caaaaaatta aagaagtgaa atttgaccgc ggtggtaacc gatttatggg tcgaattaag
    10321 gccattgccg acggcgctcg tgaaggtggt cttaaattct agatttaaat aattttatgg
    10381 aaacgactaa aacgacaact ggaaataaga aagtctttgc ccctcgcggc cgtcgcccgg
    10441 gacgccgttt tggcgagagg gaaaagccgg agtttgacca aaaagtcatc aacgttcgcc
    10501 gcgtggcgcg ggtcatggct ggaggtcgcc gtttcagttt ttcagtcgtg gttgttgccg
    10561 gtgaccgtaa tggccgcgtt ggagttggga ttggcaaggc cggcgacacc tcacaagcga
    10621 tagaaaaagc ttacaacaag gccaaaaagc aaatgattaa agtggctttg accagcaaca
    10681 aatccattcc gcatgaagtg gaggccaagt tcaaatcggc gcgaatcagt cttcgcccag
    10741 ccccaggccg tggtttggtg gccggcagtt ctgtcagaat tgtgcttggt ttgggcggtg
    10801 tcaaagacgt gaatgccaaa attgtttctc gcagcaagga taagttaaat attgcccaag
    10861 cgacaattaa ggctttgcaa gaagtgagca agggcaaaaa ataaaaaaca acatgcagat
    10921 tcaagatgta aaacgaaata cgccccaaaa aaaatctcgc cagattggtc gaggtggcaa
    10981 acgtggcacg accgcaggcc gtggcaccaa gggtcaaaaa gcccgcgcgg gtaacaaaat
    11041 tcgtcccaac cttcgcgacc aaataaagaa gattcctaag ttgcgaggtt acaaatttca
    11101 gtctaagaac gaaaagccga ctccggtcaa tttggaaaca attgaaaaat tgtttgtcga
    11161 cggcgccagt gttaatccag cgtctttggc tgagaaaaaa attatcagtc gaaagggcgg
    11221 tcgtttcccg caggtcaaag ttttgggtgg cggcactatt gccaagaagg tttccgtttc
    11281 tggttgcttg gtttccgcgg cggcgcgtga gaaaatagag aaagcgggcg gcaagattaa
    11341 ttaatgggaa aaggagaggc tgacgtttgt ttcatttaat aatccatgtt tgcgactttt
    11401 ttaaacaaat tcaatcttat tctgtcggaa cgcaacttac gcaatcggat tttgtttgtg
    11461 gtggccgcct tggccgtgtt tcgtttgggg gcgtcaatcc caataccggg cgttgaagtt
    11521 tttcaattgg aaagattttt ggcggaaaac caattcctcg gtttgttgaa cgttttttcc
    11581 ggcggtggct tgtctaccct ctccattttt atgttaggcg ttgggccata cattaccgct
    11641 tcaattatta tgcaacttct gaccttcgtc ttcccgtctc tcaagcagtt gtttcacgaa
    11701 gaaggtgaag ctggacgccg caaggtggct caatactcac gcctgctaac cgtcccctta
    11761 gcggtgattc aaggtttgtc tctgcttgtt ttgttggaaa ggcaaggtgt tttgatgaat
    11821 ttgacaatgt ttgaccgagt gacaaatatt tttgtggtgg tcgccggttc cctcttggtt
    11881 acttggattg gtgaattgat caccgaattt gggattggca acggtgtttc gttgttgatt
    11941 tttgccggta tcgtgtcgcg ttttctgccg acgttaatgc aactatggtt gacttttgat
    12001 attgcgcaag caccgatgta tttggtgttt gccttgattg cccttatcat tgttgcggcc
    12061 gtggtttttg tgacggaagc ggaaaggccc attccggtaa cttatgccaa gcgagtgcgg
    12121 gggatgaaga tgtatggcgg gatgtctact tatttgccga ttcgggttaa taacgccggc
    12181 gtgatgccaa taatctttgc cttgtctatt ttgctttttc cccagatgat tttcggtttt
    12241 ttagctggtt atgataatga atttatcagc aacgtggctt ctttcatcaa ctcgctttta
    12301 aataacaact ggctttacgc cggcctttac ttcttgttgg tgtttgcctt cacttatttt
    12361 tactcggcag taacttttga cccagaagcg atttctacca atttacaaaa aaatggcgcc
    12421 ttcattcctg gtgttcgccc agggcgttcc acggccgaat atttatctaa gattttaact
    12481 agattgactt taattggggc gattttcttg ggttttgtcg cggtgttgcc aatcttgttg
    12541 cgcagtttga ccgggattgc ttctttgtcg attggcggta ccagtctttt gattgttgtt
    12601 tcggtggcgc ttgagtttgt taaacaagtt gacgctcaag tttcaatgcg agaatactag
    12661 tctaaggcgg attaaacgca gattgctgct gataaaacgc cgacatgatg gattgtcgag
    12721 gctagatttt agaggttgca atataataat ccctgatacc gctctttgtt aatttggctc
    12781 cgggagtttt ttgcttttcc tcttaaaata tgcgaaaatt tggattatga acaccttaaa
    12841 aacctttatt ttcataggtc cttccggttg tggcaagggc actcaggttg aacttttaat
    12901 caaacatttg acgcaaactg gcgatatttc aacggaacgg ccggtgcttc attctgaatc
    12961 gggaaagcgt tttcgcgatt ttgttaagca ggctgatttt atggccggca aaaccaaatt
    13021 agcgatggag cggggcgaac gtttgccaga tgctttggcg gtctggaatt ggacgggtta
    13081 tttaatagaa cattacactg gccgagaaca cttgattttt gacggctccc cacgttcttt
    13141 gctagaggcc aaagcgttag atacgatact ctcatttcta aaacgtgaac gggcggtagt
    13201 tattttcctt gatgtttcgg ccgaagaatc aattaagcgt cttttgaagc gtgctactga
    13261 gcagggtcgg gccgatgatg atttggagga aatcaagcga cgcttggatt ggtttgaaaa
    13321 agatgtgcgt ccggcgcttg aatattatga aaaagaatcc acccatttgt ttctaagaat
    13381 aaatggcgag caaagccgag aaaagattca tgaagatatt aaagcgcgac tggctgaagc
    13441 gacgaaggcg tgatgattac tttaaaaacc caagaagaga tagaaatctt aaaagagggc
    13501 gggcggcggt tggcggttat tttacgtcaa gtggccgagt tagttaaacc ggggacagag
    13561 actgttattc ttgatgactt ggcgctgggg ttgattcgtc agggtggcga cgacccggcc
    13621 tttcttaatt atcggccatc cggttcacgc ttggcctatc cggcgtctct ttgcgtatcg
    13681 gtgaacgaag aaat
//
LOCUS       DAOOLO010000017        13401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_5068_length_13401_cov_5.798367, whole genome shotgun sequence.
ACCESSION   DAOOLO010000017 DAOOLO010000000
VERSION     DAOOLO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5068_length_13401_cov_5.798367"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1109)
                     /locus_tag="PLH96_01775"
     CDS             complement(171..1109)
                     /locus_tag="PLH96_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPB60531.1"
                     /translation="MAKANKDKIEQKLESRDFYKKLKLAQNFEKYSGGGLKRKLQRIL
                     YASKIYLPYLLVVKAGIGKKGTSKTKLFWGRKIEIPLTDFDSLALRTYGFAGGTEAEL
                     KLTKFFIKNLKQEDVFYDIGANYGFFTYLAEELCQEVHSFEPIPSLSEIIKNNSRNNT
                     RVNNLAISNQKGVTKLFMSESTGLSTINESATKIHTYSYDQAKEIEVKTDSLDAYCEK
                     FNKPTILKIDVEGAEGEVVDGATFFLQNHAPVIAMEIWGEGNGGDISKVAVARLRKLG
                     FESYQIEPTGNIQKVEGDITRLIPAMGVDNFIFKKN"
     gene            complement(1082..2371)
                     /locus_tag="PLH96_01780"
     CDS             complement(1082..2371)
                     /locus_tag="PLH96_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPB60532.1"
                     /translation="MQRLRDAVYNILRWSEKYVKTDMIYLTKGGFWLLFSYSIQAITG
                     LILSVSFANLLAKESYGFYQFVISMASILAVFSLTGIGTAIIRGIAQGDEGCLRPSVK
                     VSLKWTLGILLTAAATSLYYWTKGNYQLSIAFAVVAIAQPLITTFNLYKTYLQGKKLF
                     KSYSVIQTAQRLLPFTLLLIAILCTRNPLTLVIVYFISHAISAVLIYLYVVIKFKLPK
                     QSEVKDLVKYGKHLSLMESMAELATAADKVLIWFFLGAAPTAAYALAQLPIIHLQAMF
                     GFIRELAFPKIAQKSLPGLAKALPEKIRTYWIVALAVVVLYIISAPTIFHIFLPKYPE
                     AVIYTQVLSLVILVVPRSLVTHTFAAHQRTKELYLVNFSTPIIRLSLLAIGLPMFGIW
                     GVIGATLISEWCAALLQWKLFSNIINKISWPKQTKIK"
     gene            2444..>3710
                     /locus_tag="PLH96_01785"
     CDS             2444..>3710
                     /locus_tag="PLH96_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB60533.1"
                     /translation="MDLKKIFHWLILLGIGLVPFLPLFVSHSLLFPFISGKNFAFRII
                     VELIFALWLILAFWDKKYRPQKSLIIYSVLAFLVVVTLATIFGVSPYHSFWSNFERMD
                     GLISLLHLGAFFVVLVSILKSEDSWLKLFYVSLTANALVLFYGFGQLLGVAEIHQGGV
                     RLDASLGNAAYLATYCLFHVFIAGYLFFRHRSLRWLFVLSIIANLVILYFTATRGAIL
                     GLIGGVLLTTFILLLRNWSQPKIRKIFLFSFLAILILIALFWILKDTAFVQSSPVLSR
                     FASISLTETTTQSRFLIWQMAWQGFKEKPILGWGPGNFGYVFAKHYNPLMWRQEPWFD
                     RAHNIIFDWLINAGLLGLLSYLSILFFAFYYLGRKFWRSEKDSQESKNKQKNKLREEE
                     WREKAGTGVLVGLLAAYFFQNLFVFDNLTS"
     assembly_gap    3711..3810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3811..4854
                     /locus_tag="PLH96_01790"
     CDS             <3811..4854
                     /locus_tag="PLH96_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60534.1"
                     /translation="LLAAYFFQNLFVFDNLTSYTLFFIVLAYVHFISVGGGEGNAKPK
                     NFSDNELARLLVTSTLVGVLLVASLYQLNFKPIAAGKNVIGAIRTYPDSAINLDYYQK
                     VFAADTFVSGEAREQLLSKTLSVLDDENLPENLRNQFGQLALDEWSKQLERFSEDTRS
                     RFYFGSFLSAVGMFDQALINLQKAKELSPQKQMTRLELATLHFKMNKPDLAMAELKET
                     FELEPGYPEARKMYALGAIIVGESELAAEIIEPIKDSEEYFLDDRFVYYYNEANDRAK
                     LNDIHVKRAGYYSDLTQNNPENLENFLKLAEAQIALGQNVEAKKSLQVIIDLAKEEQK
                     TLKTKAENLLQTI"
     gene            5826..8318
                     /gene="secA"
                     /locus_tag="PLH96_01795"
     CDS             5826..8318
                     /gene="secA"
                     /locus_tag="PLH96_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPB60535.1"
                     /translation="MSIFSNILKTINDQDKKCLKEAASTVSVINALENEIQALSDEAL
                     KAKTVEFKKRLGDGETLDKILPEAFAVVRETAKRVLGQRHFDVQLVGGLILHQGKIAE
                     MRTGEGKTLVATLPAYLNALTGKGVHIVTVNDYLARRDAVWMGQIYSFLGLSVGVLNN
                     QTSYLYDPEHTDKDSLRDEEGSFRVVHEFLRPCARREAYLVDVLYGTNNEYGFDYLRD
                     NLVYDTANLVQRFKEDSHHFAIIDEVDSILIDESRTPLIISAPYAESEKLYGTFAQIA
                     SSLKAGEDYEVDEKLKAITLTDEGINKAEKKLGVENIYTEGGIKYVHHLETAVRAKAL
                     FERDKDYVVREGEIIIVDEFTGRLQPGRRWSEGLHQAIEAKEGVPIKDESRTFATITL
                     QNYFRMYQKIAGMTGTAKTSAEEFMKVYGLDVVSIPTNKPIRRIDHNDVIFQTEQGKF
                     KAIARKIKELNQKGQPVLVGTISIEKNELLSSYLKKEGVAHDILNAKNHENEGQVIAN
                     AGRKGGVVIATNMAGRGVDIKLGGATPTPEEAAEIRDLGGLFVLGTERHEARRIDNQL
                     RGRSGRQGDSGETQFFVSLEDQLMRVFASDRIKGMMGRFGIPEDEPIENSFVSRALEN
                     AQGKIEGFHFDARKHLLEYDDVTSHQRNTVYERRRKILLGGPEAVDDYLTRVLAGMTD
                     EGEREKAEKQIAAKKTTLGEEVFYTNAKRIILQVVDLFWVEHLEAIDYMRGSVRLRAY
                     GQRDPLVEFKREGLALFKNMEISAAAAALEHILNLGEQVKEEKVALDEVHESASNITA
                     GANQTREEGRKVGRNDPCPCGSGKKYKKCCGK"
     gene            9064..11364
                     /locus_tag="PLH96_01800"
     CDS             9064..11364
                     /locus_tag="PLH96_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPB60536.1"
                     /translation="MNKPFLSITKSTILGVLVLGLFLACGTPAVLMAEEVGDVLDVQI
                     VEESSGSGPGLSVTEPVETKTLEQTLDENLFENSVVETEDQNLPDPDDGSNSDEAGEP
                     EAPEGEMAIFSETATATPALSTEAEETSSTLMASSTSIGQASTTDATDFNQGAELTTP
                     VALTAGQTEVLVAISNTLGAISEALAISDEVEFTTEAVNEESETNSRVVISEEFGAGL
                     SEDSGDGHETSDDAGSENSFQTESASNESETETAVSVENIFQTEPVPVTPRKISAQMS
                     FITQAMPEVKEVISAEHYFNTLSGSVEPKVSQELTFTTADVPPPPPPPEDSEISSENS
                     FVTLDGPIIPKVSSEFSFVTSDGPPPPPPPPHWVSSEGTFVTLTYPDIPGVSEENSFV
                     TNSSGGGGGGGGGGGGGGGRRVEKEEEPELPQTCPLYLKEFIRYGRDNNPHEVIKLQL
                     FLKYFEGYDVPISGYYDLRTLRAVENFQQRYLRDVLRPWGINDPTGYVFITTTLAINN
                     IYCGRDTANSLDLRNIIGQYYQELTGEPLGLIGGSETTIPTTTVPTTTASSTPSATSG
                     PFTGEPSGVSGAQRLLAALGILADFIGDNLCWLLNLLLLAIILFLLWLLWRDKEDDDG
                     SGSTTLGGGAPAETTLTDSDKQYLSQLAREEAEKETIAADVVPTPPGVIYLGGQEKKA
                     ALAPTESEGAIPLEALFDDNEDSSLSELTEKGFEGGEGDEVSLPILGDETAEEAEEEP
                     FVNPDQESLLESEEVQADKENKSTLP"
     gene            11963..13078
                     /locus_tag="PLH96_01805"
     CDS             11963..13078
                     /locus_tag="PLH96_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60537.1"
                     /translation="MIMADEKSAEELALLALVFLAGAIHVEGGVADCVDWVYPAVVPT
                     AGGFQVEVPFSPAAKSVVARTTFQVGLGADAKKFTHTVNLLGPTPAIPTPVPFSVNIV
                     KPNDGAVLTEGKPQLFVAEAEGADEVSSWFWLFPDETVSSGSQVVAKMVSSQLSGEVS
                     VIGTTPDGRVGWDTIMVTTSPGGEEPEPETRGVEITSPVAGSTISGDSIHFHASLLGN
                     EQPTKVEWYASEGYHDTNRLDFNVPVSTFGLEPGTWSLWAKVLVTFADGTTFKDEINF
                     ALEVEESPVSAKIVAPAEGAVLPLNQEVTFKAEVSGGTPPYTSHWKFPDNDHRYNSLS
                     VVKIFAIPDSEGYVYFEVKDATGASFKTQRLVRTAAS"
BASE COUNT         3534 a         2684 c         3300 g         3783 t
ORIGIN      
        1 ccggatatat ttgccggttt agattatgtt aataaacgtg atggtgtttt tgttgtcgtc
       61 tgtcatcttc attattttga tgagaaaaag aaaaatcgtt taaatgattt aatcgccaag
      121 gcgcgagcat ttggcgctca attcagtttg cctggagaga tttttaaaac ctaatttttc
      181 ttaaagataa aattgtccac gcccatagca ggtatcagtc tggtgatatc tccctcaact
      241 ttctgaatat tgccagttgg ttctatctgg taactttcaa aaccaagttt tcttaacctg
      301 gccacggcca ctttggaaat gtcgccgcca ttaccctcgc cccagatttc catggcgata
      361 accggcgcgt gattttgcaa aaaaaatgtt gcaccatcaa cgacctcacc ttcggccccc
      421 tccacgtcaa ttttgagtat agtcggttta ttgaacttct cgcagtaagc gtcaagactg
      481 tctgttttta cctcgatctc tttggcttgg tcgtatgaat aagtgtggat tttagtagct
      541 gattcattga tggtgctcag acccgttgat tcagacataa aaagtttagt aactcctttt
      601 tgattggaga tggctaaatt attaacccgg gtgttgttcc gactgttatt ttttataatc
      661 tctgacaagc tcggaatcgg ctcaaaagaa tgaacctctt gacatagttc ttcggccagg
      721 taagtaaaga agccgtagtt ggcgccgatg tcataaaaaa cgtcctcttg cttaagattt
      781 ttaataaaaa attttgttaa ctttagttca gcttcagtcc cgccggcgaa tccataagtg
      841 cgaagagcta gagaatcaaa gtcagttaag ggtatctcta tttttcggcc ccaaaataat
      901 tttgtttttg aagttccttt tttgccaatt cctgccttaa caaccagtaa ataggggaga
      961 taaattttgg aggcgtaaag aattctttga agttttcttt ttaggccacc gccggaatat
     1021 ttctcaaaat tttgagccaa ttttaatttc ttataaaagt ctctgctttc tagcttttgt
     1081 tctattttat ctttgtttgc tttggccatg atattttatt aatgatatta gaaaacaatt
     1141 tccattgtaa caaggccgcg caccactctg aaataagggt tgcgccaatc actccccaga
     1201 tgccaaacat tggcaaacca attgccaaca aggagagacg gataattggc gttgaaaaat
     1261 taacaaggta gagctcctta gttctttggt gggcggcaaa agtgtgggtg accaatgacc
     1321 ttggaacaac aagaattact aatgacaaaa cttgcgtata aatcacagct tccggatatt
     1381 tgggcaagaa gatatggaaa atagttggag cagaaattat atacaaaacg accacggcca
     1441 gggcgactat ccagtaggtc cttatttttt ccggaagggc tttggcgagc ccgggcaaac
     1501 ttttttgggc aattttgggg aaggccaatt cgcggataaa accaaacatg gcctggagat
     1561 gaattattgg caattgggct aaggcatagg cagctgtcgg cgcggccccc agaaaaaacc
     1621 aaattaaaac cttgtcggcg gccgtggcca attcggccat tgattccatt agactgaggt
     1681 gtttgccata tttaacgagg tctttgactt ctgactgttt tggcaactta aactttatca
     1741 cgacatatag ataaatgagg actgccgaga ttgcatgaga aataaagtag acaataacca
     1801 aggtgagcgg gttgcgagtg caaaggattg cgatgagtag cagagtgaaa ggcaggagtc
     1861 tttgcgcggt ttgaattacg gaatagctct taaaaagttt ctttccctgt aagtaggttt
     1921 tatataaatt aaatgtcgtg ataagtggtt gggctatagc cacaacagca aaagctatcg
     1981 acaattggta gtttcctttc gtccagtaat aaagcgaagt tgccgctgcg gtaagtaata
     2041 tgccgagcgt ccacttaaga gaaactttga cgcttggccg taaacagcct tcgtcgccct
     2101 gggctatccc tcttataatt gctgttccga tgccggttaa tgaaaagaca gccaaaatag
     2161 aggccattga aattacaaac tgataaaatc cgtaactttc ttttgccagc aggttggcga
     2221 aagaaacgga taaaattagt ccggtaattg cctggattga ataactaaat aataaccaga
     2281 acccgccctt ggttagataa atcatgtcgg tcttgacgta tttttcgctc caacggagaa
     2341 tattataaac agcatctcgt aatctttgca tgagccttat cttacaacaa atcaaggcat
     2401 ttttctagat tttgctaaaa aaatgtggta tagtctgaat catatggatt tgaaaaaaat
     2461 ttttcattgg ttgattcttc tcggcatcgg gctggtccct ttcttgccct tgtttgtttc
     2521 ccattccctt cttttcccat ttataagcgg caagaatttt gcttttcgca taattgtaga
     2581 attaattttt gccctttggt taatcttggc tttttgggat aaaaagtata gaccacaaaa
     2641 atcgctcatt atatatagcg tcttggcgtt tttggttgtg gtcacgctgg cgacgatttt
     2701 tggcgttagc ccctatcaca gtttttggtc aaattttgaa aggatggatg gtttgattag
     2761 tctgcttcac ctgggcgcct tctttgtcgt tttggtttcc attttaaaaa gtgaggattc
     2821 ttggctcaaa cttttttatg tgtctttgac agcaaacgcc cttgttttgt tttatggctt
     2881 tgggcaactc cttggggtgg cggaaatcca ccagggtggt gtgcgtctgg acgccagcct
     2941 cggtaatgct gcctacttgg caacttattg tctttttcat gtttttatcg caggatatct
     3001 cttctttaga catcggtctc taaggtggtt gtttgttttg tcgatcattg ccaatttggt
     3061 aattttatat tttacagcca ctcgaggcgc catccttggt ttgattgggg gagttttgct
     3121 gactactttt attttactgt tgcgaaattg gagtcaacca aaaattagaa agatttttct
     3181 attttccttt ttagccattt taattttaat tgccttgttt tggatattga aagacactgc
     3241 ttttgttcaa tcaagtccag tcttgtcgcg ctttgcctca atttcgctta ccgagacaac
     3301 aactcagtca aggtttttga tttggcaaat ggcttggcaa ggttttaagg aaaagccaat
     3361 tctcggttgg ggtccgggca atttcggtta tgtttttgcg aagcattata atccgttgat
     3421 gtggcgacaa gagccatggt ttgatcgggc tcataatata atttttgact ggctgattaa
     3481 cgccggtctc ctgggcctgc tctcttactt gtcaatttta tttttcgctt tttattattt
     3541 gggacgaaaa ttttggcgtt cagaaaaaga ttctcaagaa tccaaaaaca aacaaaaaaa
     3601 caaattaaga gaagaagagt ggcgtgaaaa agctggaacg ggagttttgg tcggtttgtt
     3661 agcggcctac ttttttcaga acttatttgt atttgataac ctaaccagct nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgttagcgg cctacttttt tcagaactta
     3841 tttgtatttg ataacctaac cagctacacc ctctttttta tagtcttggc ttatgttcac
     3901 tttatctcgg ttggcggtgg ggaagggaac gcaaaaccca aaaatttttc agataatgag
     3961 ctggcgaggc ttttagttac cagcaccctg gttggcgttt tgttggttgc ttctttgtac
     4021 caactaaact ttaaacctat cgccgcgggg aaaaatgtta ttggagcaat caggacttat
     4081 cctgattcag cgattaactt agactattat caaaaagtat ttgccgccga caccttcgtc
     4141 tctggcgagg cgagagagca actcttaagc aaaacgttat ccgtgttgga tgatgaaaat
     4201 ttgccggaaa acttgcgtaa tcaatttgga caattggcgc ttgatgagtg gtccaaacaa
     4261 cttgaacgtt tcagcgagga cactcgctcc cgtttttact ttggttcatt tttgtcggcc
     4321 gtggggatgt ttgaccaagc tttgatcaat ttgcagaaag caaaagaact ctcgcctcaa
     4381 aaacaaatga ccagacttga attggcgact ctccatttca aaatgaacaa accggatttg
     4441 gccatggctg agctgaagga aacctttgag cttgagccgg gttatccaga ggcgcgcaag
     4501 atgtatgctt tgggcgcgat tattgtcggc gagagtgaac ttgccgccga gataatagaa
     4561 ccaatcaagg atagtgaaga atactttttg gacgaccgtt ttgtctacta ttataacgag
     4621 gccaatgaca gggctaaact taatgatatc cacgtcaaga gggctggtta ttattccgac
     4681 ctgacccaaa acaacccgga aaacttggaa aattttctaa aattggccga ggcccaaatc
     4741 gctttaggtc agaacgttga agcgaaaaag agtctgcaag taattattga cttagctaag
     4801 gaagagcaaa aaaccttgaa aacaaaagct gaaaacctct tacagacgat ttaagtggtt
     4861 tttaaggaca aaacccttga ttttttgttc attatttgct ataatgactc caatgtgaga
     4921 ttttcttcat aacatgggga agactccagt gctaaatgtc caaccaaagg cccggaaacg
     4981 ggcctttggt ttttgaagtt aaagtcccag cttgctggga ctttattgtt ttttaatcta
     5041 aaatatgaga agataacagt aattgaaaat attggcgacc attggtggcc gacagaggca
     5101 ctcacattag gacatttagc atatggagtt agctctctgt cggccgccaa tgagcatcaa
     5161 agagaagtct cgccgcgggc gggttttttt atttttaaaa tttaaattta ttaaaaaaaa
     5221 ttttttaaaa tcgttttttt attttcaaaa taaaaaatta gtagaaagtt ttttgtctta
     5281 aaaatttttc agaaaaattt tgaaaatgtt tttcataagt tatccacaat tttttgtaaa
     5341 aaaagattga gatgttttgt tttttatctg ataattaaga gcaggagcag cagtgttatg
     5401 attaaaggtc gaatactgtt gcaaagagct ttataaaaaa agtaaaacta taaaaaaatg
     5461 gcagcaaaga aaaaggctaa gaagccggcg aagaaaaagg tcgccaagaa aaaggccact
     5521 aaaaaagtcg ccaagaaaaa ggtagccaag aaaaagaaga aataattttc ttttctatcg
     5581 tacaccagaa aaaattccgc ctcgtgcgga atttttttct tacttggacg aaggtccaca
     5641 tagaacaagt gtggcggcgg cttatttttt aaaactaatc cactattatt tttttttact
     5701 ctatgcgggt tttaagttat cggctgtggg caatattttc ttcatttgtc atttgtttat
     5761 tgtcaaattg tcaattttgc cgcgcgttgt tttttgcctc ttttttatgt taacattagg
     5821 aaatcatgtc tatattttca aatattttga agacgataaa cgaccaggac aagaagtgct
     5881 taaaagaggc cgcgagcacg gtgtcagtta taaacgctct ggagaatgaa attcaagctt
     5941 tatccgacga agccctaaag gcaaaaacag ttgagttcaa gaagcggctg ggtgatgggg
     6001 agactttaga taaaattttg ccggaggctt ttgcggtggt acgcgaaacg gccaaacggg
     6061 ttctgggtca gcggcatttt gatgtccagc tggtcggcgg tttgattttg catcaaggta
     6121 aaattgccga aatgcgtact ggtgagggca aaaccttggt ggcgaccttg ccggcgtatt
     6181 tgaacgcgct taccggcaaa ggagtgcaca tagtaacagt caacgattac ttggcgcgtc
     6241 gtgacgcggt ttggatgggc caaatttatt cttttctcgg tctttcggtt ggagtgttga
     6301 acaatcagac ttcttatctt tacgacccgg agcataccga taaagatagt ttacgtgacg
     6361 aagaaggttc ttttagggtg gtacatgaat ttttgcggcc ttgcgcgcgt cgagaagctt
     6421 atttggtgga tgttctttat ggcactaata atgaatatgg ttttgattat ttgcgtgata
     6481 atttggttta tgataccgcc aatttggttc aacgttttaa agaagacagc catcatttcg
     6541 cgattattga cgaagtggac tctattctga ttgatgaatc gcggacgcct ttgattatct
     6601 cggcgcctta tgctgaatcg gaaaagctct atggcacttt tgcgcaaatc gcttcctcac
     6661 tcaaggcagg ggaggattat gaagtagatg aaaaactgaa ggccatcact ttgactgatg
     6721 aaggcatcaa caaggccgaa aaaaagctcg gggtggagaa tatttatact gaaggtggaa
     6781 ttaagtatgt gcaccatctg gaaacggctg tgcgcgctaa agcgcttttt gagcgagata
     6841 aagattatgt ggtgcgcgaa ggtgaaatta ttattgtgga tgaatttacc ggccgtcttc
     6901 aaccggggcg acgctggtcg gaaggtttgc atcaggcgat agaagccaaa gaaggagtgc
     6961 cgattaaaga tgagtctcgc acttttgcga cgattacgct ccagaattat tttcgaatgt
     7021 atcaaaaaat tgccggtatg accgggactg ccaagacttc tgcggaagag tttatgaaag
     7081 tttacggctt ggacgttgtc tccattccaa ccaacaagcc gataaggcgt attgaccata
     7141 atgatgtcat atttcagact gagcagggta agtttaaggc gattgcccgt aaaatcaaag
     7201 aactgaacca aaagggtcag ccggttttag tcggcaccat ttccattgag aaaaatgaac
     7261 tcctctcttc ttacttaaag aaggagggcg tggcgcacga tattttaaac gccaagaacc
     7321 acgaaaacga aggtcaggtg attgccaacg ccgggcggaa gggtggagtg gtgattgcca
     7381 ccaacatggc cggtcgcggt gtagacatca agttgggggg agcgactccc accccagagg
     7441 aggcggcaga gatacgagat ttgggtggtt tgtttgtgtt ggggactgaa cgtcatgagg
     7501 ccaggcgaat tgacaaccaa ctgcgcgggc gttctgggcg gcagggcgat tcaggcgaga
     7561 cccaattttt tgtttctctg gaagatcaac taatgcgcgt ttttgcctct gaccgtatca
     7621 aggggatgat gggccggttt gggattccgg aagatgaacc aatagaaaac agttttgtgt
     7681 cgcgcgcgtt ggaaaatgcg caaggcaaga ttgagggatt tcactttgac gcgcgcaagc
     7741 acctgttgga atatgacgac gttaccagtc accagcgtaa cactgtttat gagcgtcggc
     7801 ggaaaatttt attaggtggg ccggaggcgg tagatgatta tctgacgcga gttttggccg
     7861 gaatgaccga tgagggcgag cgagaaaagg ccgaaaaaca aattgcggcc aaaaaaacca
     7921 ctctgggcga ggaggtgttt tataccaacg ccaagcggat tattctgcaa gtggtagact
     7981 tgttttgggt ggaacacttg gaggcaattg attatatgcg aggtagcgtg cgtttgcgcg
     8041 cctatggcca acgcgacccc ctggtagagt ttaagcgcga aggcctggct ttgtttaaaa
     8101 atatggaaat atcggcggcc gctgcggcct tggaacatat tctaaattta ggggagcagg
     8161 ttaaagaaga aaaagtggca ctggacgaag tccatgagtc agcttccaat atcacggctg
     8221 gtgccaatca gacgcgcgaa gaaggaagaa aagtgggtcg taatgatccc tgtccgtgcg
     8281 ggagcgggaa gaaatacaaa aagtgttgcg gtaaataaga cagggtttcc cgtcgcctaa
     8341 gcccgtctta gagcaagtta aaaaagaaaa aagtaaggcc ggagcgggaa gtcccgactc
     8401 cggccttttg acccaatttt gggcaaagtc acaaagtttg ggttttggca tcggagatgc
     8461 aggcaagtct tcgcgaattt attctttgca accaccttct gaatcttttc ctcgttcgct
     8521 cgagtctccc cagcgggttc cttggcgggt ggtggtgact accctcaccg aaacccttag
     8581 tttcggatac tcttgttcta ttattgagca tgggtcctcc ctcctttctt tttggcgctt
     8641 gggcaagagc tatttcaaaa ctgattttag catgagaatt tttattgggg gggacatttt
     8701 tcccgtagtt atccacaggc ggtgaagtgg aactgggaaa caaaagaaac agaaaacaaa
     8761 tggagcgtcg gaaaattgga tgaagaaatt tataggacag aaatctaaag gacataaagg
     8821 acacgtttga aaaacccttg ttttacaacg tcttttagac ttgctttgtt tttttgaatt
     8881 tttacctgaa ttttggctta agattggtcc aacattaatt gacaaagtac ttgatttttt
     8941 attgttgttc tgttaggaat taaatcagtt aaaggttcag cttctgggga gaagcgtttg
     9001 ggcctctgat ccgctctttg aaatgatttg ttcatttgtg actatatttt ttctgttacc
     9061 tttatgaaca agccatttct atcaataacc aaatcaacta ttttgggagt cctcgtacta
     9121 gggctgtttt tggcgtgcgg aactccggcg gtattgatgg ccgaagaagt aggcgatgtt
     9181 cttgatgttc aaatagtaga ggaatcgtcc ggttcaggac ctggactgtc tgttacagag
     9241 ccagtggaaa caaagacgct ggagcagacc cttgacgaaa acctatttga gaattcggtt
     9301 gttgaaaccg aagaccagaa tttgccagat ccagatgatg gctcaaattc tgatgaagcg
     9361 ggtgagcctg aagcgccaga aggcgaaatg gcaatctttt cggagacagc aacagcaact
     9421 cctgccttat ctacagaagc agaagaaact tcttctactt tgatggctag ctctacgagc
     9481 atcggccagg ctagcactac cgacgctact gacttcaatc agggtgccga gctcacgact
     9541 cccgttgctt taaccgcggg acaaaccgaa gttctggtgg ctatcagtaa tactttgggg
     9601 gcaatcagcg aagctttggc aatcagcgat gaggtggaat tcacgaccga ggctgttaat
     9661 gaagaatcag agactaacag tcgggttgtc atcagcgaag aatttggggc tggcttgtcc
     9721 gaagattcag gagatggcca tgaaactagc gatgatgctg gtagcgaaaa tagcttccaa
     9781 acagaaagcg cttcaaacga gagcgaaacc gaaacggcgg ttagcgttga aaatattttt
     9841 caaacggaac ctgtcccggt cacgccaaga aaaatcagcg cccaaatgtc ctttatcact
     9901 caggccatgc ccgaagtaaa ggaggttatt agcgctgaac attattttaa tactttgagt
     9961 ggttcggttg agccaaaggt gagtcaagaa ctgacgttca ccacagccga tgtcccgcca
    10021 cccccgccac cccctgaaga ctcggaaatt agctccgaga actcttttgt tactttggac
    10081 ggaccgatta tccccaaggt cagctcagaa ttttctttcg tgacgtctga tggtccaccc
    10141 ccacccccac cgcctcctca ttgggttagt tcggagggga ccttcgttac tttgacgtac
    10201 cctgatattc ctggtgtcag tgaggagaat tcttttgtca ctaattcttc cggcggtggc
    10261 ggaggcggag gcggtggcgg aggcggaggt ggaggtcgtc gtgtggaaaa ggaagaagaa
    10321 ccagaattgc ctcaaacctg tccgctttac ttaaaagagt ttattcgtta tggccgcgac
    10381 aataatccgc acgaggtcat caaacttcaa ttgttcttaa agtattttga aggttatgac
    10441 gtgccgataa gcggttacta tgacttgcgg accttgcgcg cggtggaaaa tttccaacag
    10501 cgttatctgc gtgatgtctt gcgtccctgg ggaattaacg atccgactgg ttatgtcttt
    10561 atcacaacta ctttagccat caataatatt tattgcggtc gcgatactgc taactcatta
    10621 gatttacgaa atattattgg acaatattac caagaattga ctggtgagcc tttgggattg
    10681 attggcggct cggaaacaac tatcccaacc acgaccgttc caaccacgac agcttcttca
    10741 actccttccg cgacctcggg gccatttact ggtgagccaa gcggagtttc cggcgctcag
    10801 cgtttgttgg cagcccttgg tatcttggct gacttcattg gtgacaattt gtgttggctt
    10861 ttgaacttgc ttctgttggc tatcattcta ttcctgctct ggttattgtg gcgagacaaa
    10921 gaagatgatg atggatcagg ctcgaccact cttggtggtg gagcaccggc agaaacaacc
    10981 ttaactgatt ctgacaaaca atatttaagt caattagcgc gcgaagaagc agaaaaagaa
    11041 actatagcgg ctgatgtcgt tcccacgccg cccggagtaa tttaccttgg tggtcaagaa
    11101 aaaaaagcgg ctctggcgcc gactgagtcc gagggcgcga ttccgcttga ggcgctgttt
    11161 gacgataatg aagattcttc tttatccgaa ttgaccgaaa aaggatttga gggtggagag
    11221 ggcgacgaag tcagtttgcc aatcttgggg gatgaaacag ctgaagaagc cgaagaagag
    11281 ccttttgtaa accctgacca agaaagtttg ttggagtctg aagaggttca agcggacaaa
    11341 gaaaacaaat caacactacc ctaagaaaag taggtggcta aaacgcaaaa aagcgtgaaa
    11401 taaacaccgg ctggattcag ccggctctta aatgagggaa ggttaaagat ttcgcggtct
    11461 ttgtttggac ggctgcttat ttagttgaca ttttgcttat tttttgttat gcctgggtgt
    11521 agatgaaggc tggttttagc tggcctggtt atttgaaaag aagggggatc gggtggtccg
    11581 aaacccttgt gccgtgttgg cccgctaatt ggcgggaaat tccgccaatc ggcggaggga
    11641 ggttcccatg aatacaatga aaggtttggt cgtggtgttg acgttggtgt cgttggtcgt
    11701 cggttgcccg gacccgggag aagtcccacc ggtttccttc gactatgata ttcaggggac
    11761 tcttttccag gacggattgg ccgttcggga gttcgagaac atgggccaga gcgctgacgt
    11821 tcaggtctcc ggtcctggcg tcttggtctt ggacttttgg tccgagaaag gcgccttgga
    11881 agtgggcacc gctctggagg tccactataa acaggagttc ggagggggga tcctctgggt
    11941 ctctctgaca gaagagggac cagtgatcat ggctgacgaa aagtcagccg aggagctggc
    12001 cttgttagcc ctcgttttct tggcgggggc aatccacgtg gaaggcggag tggccgactg
    12061 cgtggactgg gtttacccgg ctgtcgttcc aacggcgggc gggttccagg tggaggtgcc
    12121 gttttcgccg gctgccaagt cggtggtggc gagaaccacc tttcaggtgg ggcttggcgc
    12181 ggatgccaag aagtttactc acacagtaaa tttgcttggc ccgacgccag ctattccgac
    12241 cccggttccg ttttccgtca acattgtcaa gccgaatgac ggcgcggtgt tgacggaagg
    12301 gaaacctcag cttttcgtgg ctgaggcgga gggggccgac gaggtctctt cctggttctg
    12361 gctctttccg gatgagaccg tctcgtccgg aagccaggtg gtcgccaaga tggtctcctc
    12421 ccagttgtct ggtgaagtgt cggttatcgg cacgacccca gacggacggg ttgggtggga
    12481 tactatcatg gtaaccactt cgcccggggg tgaggagccc gagcccgaaa cccgcggggt
    12541 ggaaatcacc tcgccggtag ctgggtcaac catttcgggt gactccattc atttccacgc
    12601 ttcgttgctt ggaaatgagc agccgacgaa ggtggagtgg tacgcctctg agggctacca
    12661 tgacactaat cggctagact tcaatgttcc ggtttcgacc ttcgggttgg aaccagggac
    12721 ctggagtctc tgggcaaagg tcctcgtgac cttcgccgac ggcacaacct tcaaggacga
    12781 aatcaatttc gccttggagg ttgaggaatc gccagtgtct gccaagatag tagctccggc
    12841 agagggggca gtcctgccct tgaaccagga agttactttc aaagcagagg tgtcgggtgg
    12901 aaccccgccc tacacgtctc actggaaatt ccccgacaat gaccaccggt acaattcatt
    12961 atcggtggtc aaaatcttcg ctattcccga tagcgagggg tacgtctatt tcgaagtgaa
    13021 ggacgcgacg ggggcgtcct tcaagacaca gcggctggtg aggaccgccg catcctaatt
    13081 gttctttact cgcgcccact ttcgggtggg cgcgagtttg ttcataataa ttgtctgaaa
    13141 aatgctgttt tttgactgat ttcctttgtt aatcaaccgt tcatttagag gtatcaggtt
    13201 tatgccacag ctcttgaata aattaagaat ctgtgctagg aataaaacaa gagcctgatg
    13261 gttcaatttt cattaattac caaggcccaa agccgaaaat tacaaagatt aataattata
    13321 ggtatgaaaa aagtaacatt tatcgctttg atgctagccc tcgttttggc cggtttttgg
    13381 accgcgtctc caatgagcgc c
//
LOCUS       DAOOLO010000018        12771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_5415_length_12771_cov_7.113636, whole genome shotgun sequence.
ACCESSION   DAOOLO010000018 DAOOLO010000000
VERSION     DAOOLO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5415_length_12771_cov_7.113636"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLH96_01810"
     CDS             <1..500
                     /locus_tag="PLH96_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPB60538.1"
                     /translation="MSDYIKEGKVAVAFRHFPLDSIHPSARKLAVAAECAGKLGGSEK
                     FWQFIDFVFAAPDKSAESLVADATEAIGLDIETMTSCQAEKEISDAVESEYQEATGLG
                     AQGTPFIAVVGPDDLVMPIFGGREAKDLKQAIDMILADEAEPIVETEIETEIEEAAAT
                     SSATE"
     gene            complement(560..1846)
                     /locus_tag="PLH96_01815"
     CDS             complement(560..1846)
                     /locus_tag="PLH96_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB60539.1"
                     /translation="MMKTEKKKVAAKKKTVKKVTKKIVKKTSVKKAKLAKKKPISKKT
                     PRKSVRRPSRAKGPAVIIHKKELADKTKTTAEKIDQKVARLVSKGRQRHFVTYTELLK
                     EFPNIENDIIFLDELYAKLEELSIDVLEEGGLLDDESLLNVKDKVKDSRDSSLDSVQM
                     YLREIGRHKLLTGAEEKELARKILAGDEDAKKSLAKANLRLVVSIAKKYVGRSSDLSL
                     LDLIQEGNIGLFKAVEKFDWRKGYKFSTYATWWIRQAVTRALADQSRTIRIPVHMVET
                     IAKYKQVFRRLSQDLGRDPLPQEIATEMDMEVEKIHNIEKIDQSTASLDKPTDDDDEK
                     SSLGEFIADDKIAAPDQDVSRRILRDQIEEIMDDLTPKERKILEMRHGLMDGVMRTLE
                     EVGKEFGVTRERIRQIEAKAHEKIRQHEKAKKLLGY"
     gene            complement(1879..3588)
                     /gene="dnaG"
                     /locus_tag="PLH96_01820"
     CDS             complement(1879..3588)
                     /gene="dnaG"
                     /locus_tag="PLH96_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB60540.1"
                     /translation="MSSSVEQIKAKLDIVEVVSAYVKLEKAGVNWRGRCPFHNEKTPS
                     FFVSPPRGSYHCFGCNKGGDMISFVQDIEGLDFVGALKLLADKAGIELEPFERKNRDE
                     NDKLRRVLETAEIFYRNNLAAEKEVVKYLLDRGLTLETIKKFGLGFARDKWRELGDFL
                     NDKGFDSEVLEKAGLSVRSSSAHASRPYDRFRNRVMFPIRDQAGRVVGFSGRIFAAGD
                     DVAKYINSPQTAVFDKSKILYGFDLAKQEIRKRDFVVLVEGQVDLVMSHQAGAINTVA
                     TSGTALTEEHLKLIKRLTDNLILAFDFDLAGLKASWRAINMARMLELNVKIAKLPKNT
                     DPADLIKQNPVAWTKAVAEAAHYIDFMIEALKQEGKTGLDLTLAVNEYVLPYLKVLGK
                     KMEQAHFVTKLSTWLGLPEQAIWQDLDKVQVLSEMPAVAKTQTPPKIRNRQEVIEERI
                     FGLWWGNEGKEFWLKGLELLKDLFGDELLAKKEKELIEQKDRLILEAELLYGDKDLNK
                     VFAELLSGLKEEMKKAELAFFMSQVKQAEKAGDEKLLELSLKKCQELSAAINENRKEF
                     NKL"
     gene            complement(3678..7364)
                     /gene="rpoC"
                     /locus_tag="PLH96_01825"
     CDS             complement(3678..7364)
                     /gene="rpoC"
                     /locus_tag="PLH96_01825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB60541.1"
                     /translation="MTNAALETGIKSDKATDFDMITLRLASPERILEWSHGEVTKPET
                     INYRSGRSERSGLFDERIFGPEKDYECYCGKYKRIRYKDIICEKCGVEVTRSIVRRER
                     MGHIELACPVAHIWFVRGVPSRLGLALDLTIADLEKVIYFAGYIIIKVNDDEKSKMLK
                     ELDNEYRQKSKGLTNEEDKENLKNAFMEAKRQLENITPWRVIDEVLYHRLSLKYGTVF
                     EAGIGAEAIYEILRTLDLEEMENNLTGNLTKASSVERVKLQKRLNLYRAMRRSGVRPE
                     WMFLKLVPVIPPALRPMVALDGGRHATSDVNDLYRRVINRNNRLKKLVELNAPEVILR
                     NEKRILQEAVDSLLDNSMRQGSGGGAMSQAQRRPLKSLADNLKGKQGRFRQNLLGKRV
                     DYSGRSVIVVGPDLQLDQCGLPKHMALELFRPFIISKILERELAYNIRGAGRLIDEGS
                     PEVWAILEEVIKNKYVLLNRAPSLHRLSIQAFRPVLIEGNAIQLHPLACSAFNADFDG
                     DQMAVHLPLSDIAQKEAQDIIASNRNILKPGSGDPVVNMRLDIVLGCYWVTKIIKGEK
                     GEGNYFANPHEAILAYDFGEVSLRAKIFVKATEKERYAEFNGQVFETSIGRLLFNNNL
                     PADYAFINKDINGKLINKLVEDMIARYGVEETAKIIDRIKKFGFRYSTKSGITWGIDD
                     VQVPEEKAKIIEDAKKEVAIIEDNYQDGLLSSNEHYRMIIEVWNNAKGKVEKAVPNSL
                     PEGGSVSYMIDSNARGSISQVVQMAGMKGLIISPAGRLIPFPIIPCYKEGLSPLEYFI
                     TTHGSRKTLADTALNTAKAGYLTRKLVDVSQDVIVTEEDCGDKDGKKITKENASSFDT
                     GIGNLITGRILAKDLISADGKTLFKRGHLMTKVEAALAEEAGASEAYVRSPLACKSLK
                     GLCRHCYGLDMGRGEMVKIGESVGIVAAQAIGEPGTQLTMRTKHSGGIGTGGVDIVGG
                     LPRVEEIFERRNPKNPALISTVDGEVSEIKNTGKEYIVSILVDPSSRKKPSDSTTELT
                     VPFVRTLLVKKGDKVKKGQIISDGPAELPLLFKLAGKDRTEDYIMKEINAIYELQGVN
                     IARKHIELIVRQMFSRLKIKNPGDTKFAVNEVVELIELVEENEKAKAEGGEEAIAENV
                     LLGISEVALTTASFLSAVSFQHTTKVLIDTAIKGGKDKLRGLKENVIIGRLIPAGTGL
                     IKDYGKELEEDEDDFSAVSAEEAE"
     gene            complement(7372..10563)
                     /locus_tag="PLH96_01830"
     CDS             complement(7372..10563)
                     /locus_tag="PLH96_01830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB60542.1"
                     /translation="MAKLMKQKYFSRYKKPMAEMPDLVEVQKKSFRWLIEEGLKELFK
                     EFTPIKDYSEKKFELEFTGFQLDEPKFDEYYARDNNLSYDAPLRVKVNLRNKTISQDK
                     EQEIFLVDFPLMTSHGTFIINGIERVIVPQLARSFGVFFTANEIKGHKFFGAKIIPAR
                     GAWVELETEIDDVIYVRIDRKRKFPVTSLLRIFADKEGREISDEELIKVFADQKIDTT
                     FIEKTLAKDHAKTASESYIEVYRRLRDSDLATAANSREFIGGMFDREKYDLSVVGRVR
                     FNERFGKKEVDKDTEERVLTFDDIVKIVSHIITLNTTKGAEEDDIDHLGFRRVRCIGE
                     MLQAKLKIGMMQMKRNIQDRMSTVDTSTLLPIQIISPRPLQARLKEFFSTNQLSQFMN
                     QENVLAELEHLRTLSALGPGGLTRERAGLEVRDVHSSHYGKVCPIHTPEGPNIGLILH
                     LSAYARVNDFGIIEAPYRKVEKGKITGEIVYLNALEEEKYVVVHAGANYAEDGSLLSE
                     YVEARQKGMPGLYKKTDVHFIDVAPNQAYSIATSMIPFLEHDDANRALMGSNMQKQAV
                     PCIIPEVPLVATGAEARAAFDSGRLILAQSSGTVSRADGRHIVVKREDGKEDVYNLVN
                     FKMTNDYTCFHQRPTVAVGQKVKKGDLLADASSTDNGQTAVGQNVFVAFMSWGGTNYE
                     DAIILSERLVKNSKFTSVYIEEFEVNVRDTKLGPEVTTHDIPNVGEVKLRNLDEDGIV
                     RIGAEVEQNDILVGKITPKGETELTPEERLLRAIFGEKARDVKDSSLRMPHGRKGRVI
                     GVKVFSRERGDKLESGIIKKIKIQIAQIRNVSVGDKLAGRHGNKGVISRILPEEDMPF
                     MADGTPVDIILTPLGVPSRMNLGQIFEMHLGLAANSLGYQAITPVFMGATENEIKDEL
                     QKAGYSREGKVTLFDGQTGEKIEKDIAIGYMYMLKLAHMVEDKIHMRSIGPYSLITQQ
                     PLGGRSQGGGQRFGEMEVWALEGYGAAHTLREMLTIKSDDITGRSSTFDSIVKGESIK
                     QPNLPASFNVMLNELRGLALDIELKKNNKDDHE"
     gene            complement(10785..11492)
                     /gene="trmD"
                     /locus_tag="PLH96_01835"
     CDS             complement(10785..11492)
                     /gene="trmD"
                     /locus_tag="PLH96_01835"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPB60543.1"
                     /translation="MVFHIITIFPESFESYLNSSMLWRAQKEKKVKFKFYNPRNYTKD
                     KHRKVDGRPYGGGPGMVLQVEPILLAVKAAQKNIARSVKPARFVKTPNLKSKSFNLTS
                     KIIVTGPAGKQFDNGVADQWSRKLGHLIIICGHYEGIDARVKKILRAEEISIGPYVLT
                     GGELPALVMMDAVARRIPGVLGNEVSLEEKRIAASEVYTRPEVYKHGDKNYRAPKVLL
                     SGHHKKMEEWRKTRAKR"
     gene            complement(11618..11947)
                     /locus_tag="PLH96_01840"
     CDS             complement(11618..11947)
                     /locus_tag="PLH96_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPB60544.1"
                     /translation="MAEMHKDQEFLEYVVKSLVDSPEDVKVDRKVDEMGVLMTLEVNP
                     VDMGKIIGRQGNTAKAIRTLLRVVGMKFNARVNLKINEPVGAANNMATSSASRSVDQA
                     MEDLKQI"
     gene            complement(12098..12568)
                     /gene="rpsP"
                     /locus_tag="PLH96_01845"
     CDS             complement(12098..12568)
                     /gene="rpsP"
                     /locus_tag="PLH96_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPB60545.1"
                     /translation="MIRLQRVGRKNSPSFRAVVVDSHAAAKTGKVIEVVGNYDARHGE
                     PSLKADRILDWLGKGAQVSDTMHNLLVKKGVVKGTKKNVASGKLGKKARAAKESKEKA
                     EAAQKAAKAAEAEKPIEPIAEEGNTETPTEETKTEELPEASTEETPVEEEQAAE"
BASE COUNT         3342 a         3240 c         2550 g         3639 t
ORIGIN      
        1 aaatgagcga ttatataaag gaaggaaaag tagcggttgc ttttcgtcac ttcccgcttg
       61 actccatcca tcccagcgcc cgaaagttag cggtcgcggc agaatgcgcc ggcaaattgg
      121 gggggagtga aaaattctgg caatttattg attttgtttt tgccgccccg gacaaatccg
      181 ccgagagttt ggttgccgat gcaactgaag ccatcggttt ggacattgag acaatgacca
      241 gttgccaagc cgaaaaagaa atcagtgacg cggttgagtc tgaatatcaa gaagcgaccg
      301 gcttaggcgc gcaaggcact ccttttattg cggtagtcgg accggatgac ttggtaatgc
      361 ccatcttcgg cggacgagaa gccaaagacc tcaaacaagc gattgacatg attttagctg
      421 atgaagcaga gcccattgtc gaaacagaga ttgagacaga aatagaagaa gccgccgcca
      481 cctcatcggc cacagaataa acaattttac cgacaaaaat cgcctccatg atggaggcaa
      541 tttttgtgtt tttgatttat taatagccga gtaatttctt ggctttttca tgctggcgaa
      601 ttttttcgtg cgccttagct tcaatctggc gaatgcgttc acgtgtcacg ccaaattctt
      661 tgccaacttc ttccaacgtt ctcattaccc catccatcag tccatgtctc atctccagga
      721 tttttcgttc tttaggcgta aggtcgtcca taatctcttc aatctggtct cgtaaaattc
      781 ggcgcgaaac gtcctggtcc ggcgcggcga ttttgtcgtc agcgataaac tcacccaagg
      841 acgatttttc atcatcatca tcggttggtt tgtcaagcga agccgtgctt tggtcaattt
      901 tttcaatatt gtgaattttt tccacctcca tatccatctc cgtggctatt tcttgcggca
      961 agggatcgcg tcccaaatct tgggacaaac ggcgaaaaac ctgcttatac ttagcgattg
     1021 tctccaccat gtgaaccgga attctaattg ttctggactg gtcagccaag gcgcgagtaa
     1081 ccgcttgtct aatccaccaa gtggcataag tggaaaattt gtaacccttg cgccaatcaa
     1141 atttctctac tgccttgaaa aggccgatgt tgccttcttg gataaggtca agcaaggata
     1201 agtccgaact gcgaccaaca tattttttgg cgattgaaac aactaaacgt aagttagctt
     1261 tggccaaact cttcttagca tcctcatccc cagccaaaat tttacgcgcc aattcttttt
     1321 cttcggcacc ggtcaaaagc ttgtgccggc caatctcacg cagatacatc tgaactgaat
     1381 ccaaagaaga atcccgactg tctttgacct tgtctttcac gttcaaaagg gattcatcat
     1441 ccaagagacc cccctcctcc aagacgtcaa tgcttagctc ttccagttta gcgtaaagtt
     1501 cgtcaagaaa aataatatca ttttcaatat tcggaaactc ctttaagagt tccgtatagg
     1561 tcacaaagtg gcgttggcgt cccttgctta ccaggcgcgc gactttttgg tctatctttt
     1621 cggccgtcgt tttagtttta tcagcgagtt ctttcttgtg gataattacc gccggccctt
     1681 tggcgcgaga cggtctcctg accgactttc gcggagtttt tttggatatt ggtttctttt
     1741 tggccaactt cgcttttttg acggaagttt ttttcacgat ttttttggtg acttttttaa
     1801 ccgtcttctt tttagctgcg acttttttct tttctgtttt catcataaaa ttgttgtcat
     1861 tttaaattat tcaaatattt ataacttgtt aaattctttg cgattttcat taatggcggc
     1921 agataattct tgacactttt ttagacttaa ctccaacaat ttttcatctc ccgctttttc
     1981 ggcctgctta acttgcgaca taaaaaaagc cagctccgct tttttcatct cctcttttag
     2041 gccagacagt aactccgcaa aaaccttgtt taaatccttg tcgccatata agagttccgc
     2101 ttccaaaatc aaccggtctt tttgttcaat taattctttc tcctttttag ccaatagctc
     2161 atcaccaaac aagtccttga gtaattccaa ccccttcaac caaaattctt taccttcatt
     2221 cccccaccaa agcccaaaaa ttctttcctc tatcacttct tggcgatttc taatcttggg
     2281 gggcgtctgt gttttcgcga ccgctggcat ctcactcaga acttgcactt tatccaaatc
     2341 ttgccaaatc gcctgctcag gcaaaccaag ccaagttgac aatttggtca caaaatgggc
     2401 ttgttccatt tttttgccca aaaccttgag gtaaggcaag acatattcgt tgaccgccag
     2461 ggtcaagtcc aaaccagtct tcccttcttg ttttaacgcc tcaatcataa agtcaatata
     2521 gtgagcagct tcggcaaccg cttttgtcca agcgaccgga ttttgtttga ttaagtcggc
     2581 ggggtccgtg tttttgggca acttggcaat tttgacattt aactcaagca tgcgcgccat
     2641 atttattgct cgccaagagg ctttgaggcc agccaaatca aaatcgaaag ccaaaattaa
     2701 attgtctgtt agacgtttta taagcttcaa gtgttcttcg gtcagagctg tgccagaagt
     2761 agcaaccgtg tttatggcgc cggcctggtg ggacatcacc aaatcaacct gcccctccac
     2821 caataccaca aagtctcttt ttctgatttc ctgcttggct aagtcaaaac catagagaat
     2881 ttttgactta tcaaaaaccg ctgtttgcgg gctatttata tatttagcaa cgtcgtcacc
     2941 ggcggcgaat atacgaccag aaaaaccgac aacccgcccg gcctgatccc tgataggaaa
     3001 catcacccgg ttacggaaac ggtcgtaagg tcgactggca tgggcggagc ttgacctgac
     3061 cgaaagaccg gccttttcca aaacctcaga atcaaatcct ttgtcattta gaaaatcccc
     3121 caactcgcgc catttatcac gcgcaaaacc aagaccaaac tttttgattg tttccaaggt
     3181 cagtccccgg tcaagcagat atttaacaac ttctttttcg gccgccagat tgttgcgata
     3241 aaaaatttcc gctgtttcca aaacgcgccg caatttatca ttttcatcgc gatttttcct
     3301 ttcaaacggt tccaactcta tgcctgcctt gtcggccaaa agtttgagag caccgacaaa
     3361 atccaaacct tcaatatcct ggacaaaact aatcatatcg ccacccttgt tacaaccaaa
     3421 gcaatgatag gaaccgcgcg gcggcgagac aaagaaagat ggggttttct cattgtgaaa
     3481 aggacaacgc ccgcgccaat tgaccccggc cttttccagt ttaacataag cgctgaccac
     3541 ctccacaata tccagcttag ctttaatttg ttctactgag gatgacattt ttatggttag
     3601 gcaaaacagg agcgacagac ggccaaacgc ctaccgtctg acagctccac ttattaccct
     3661 gttggtgttc aataagacta ctccgcctcc tccgcgctaa ctgccgaaaa atcgtcttcg
     3721 tcttcttcga gttctttgcc gtaatctttg atcagaccgg taccggccgg aatcaaacga
     3781 ccaataatca cattttcttt taggcctcgc agtttgtctt ttccgccttt aatggccgta
     3841 tcaatcaaaa ccttggttgt gtgttggaaa gaaacagccg acaagaaact ggcggtggtt
     3901 agagccactt cggaaatgcc gagcaaaaca ttttcggcta tcgcctcttc gccgccttca
     3961 gcttttgcct tttcattttc ttcaaccaat tcaatcaatt caacgacttc attcactgca
     4021 aatttggtgt ctccaggatt tttaatcttt aggcgagaga acatttgtct gacaatcaac
     4081 tcaatatgct ttctggcgat attcacgcct tgcaactcat aaatggcatt gatttctttc
     4141 ataatataat cctccgttct gtctttgccg gccaatttaa acaataacgg caattcggct
     4201 gggccgtcgg aaatgatttg tccttttttg accttgtcac cttttttgac caacaatgtt
     4261 ctgacaaatg gcacagttaa ttcagtggtg ctatcactgg gtttcttgcg actagacggg
     4321 tcaaccaaaa tggaaacgat atactcttta ccggtgtttt tgatttcaga gacttcacca
     4381 tcaacggttg agataagagc cggattctta ggattgcgtc tttcaaaaat ttcttccact
     4441 cgcggcaaac caccaacaat gtccacaccg ccggtaccaa taccgccaga gtgtttggtc
     4501 ctcattgtta actgagtgcc aggttcacca atcgcttgag cggccacaat tccaaccgac
     4561 tcgccaattt tcaccatttc gccacgaccc atatctaaac catagcaatg acggcagagc
     4621 cccttaagag acttacaggc caacggcgaa cgaacatagg cctcagacgc gccagcttct
     4681 tccgccaagg ctgcctccac cttggtcatc agatgacctc gcttaaagag agtcttgccg
     4741 tccgcgctaa tcaaatcttt ggccaaaatt cgcccggtaa tcaagttgcc gataccagtg
     4801 tcaaacgaag acgcgttttc tttggtaatt tttttaccat ctttgtcgcc gcaatcttct
     4861 tcagtaacaa taacgtcttg ggaaacatca accagctttc gtgtcaggta gccggccttg
     4921 gcagtgttta acgccgtgtc agccaaagtt ttgcgcgaac cgtgagtcgt aataaaatat
     4981 tccaacggtg acaaaccttc cttataacaa ggaatgattg ggaaaggaat taaacgaccc
     5041 gccggactaa taattaaacc tttcatgccg gccatctgga cgacctgact aatagaacct
     5101 cgcgcattag aatcaatcat ataagacacg gaacctcctt cgggcaaact gttgggaacc
     5161 gctttttcca cctttccttt ggcattattc caaacctcaa ttatcattcg ataatgttca
     5221 ttggaagaca gaagaccatc ctgatagttg tcttcgataa tcgccacctc ttttttggca
     5281 tcttcaataa ttttagcctt ttcttcgggc acctggacat catcaattcc ccaagtgatg
     5341 cccgatttag tggaataacg aaaaccgaat ttttttattc tatctatgat tttggcggtt
     5401 tcttccactc catagcgagc aatcatatcc tccaccaatt tatttatcag ttttccatta
     5461 atgtctttgt tgatgaaagc gtaatcggca ggcaagttgt tgttaaacag aagacgtcca
     5521 atggaagttt caaaaacttg accattaaac tccgcataac gttctttctc ggttgcctta
     5581 acaaagattt tggcacgcaa actaacctcg ccaaaatcat aggccaaaat cgcctcatgc
     5641 ggattggcaa aataattacc ctcacctttt tcacctttta taattttagt cacccaataa
     5701 caaccgagaa caatatcaag tctcatgttg acgaccggat caccgctgcc cggttttagg
     5761 atattccgat tagaagcaat gatgtcttgc gcttcttttt gcgcaatatc agacagtggc
     5821 aagtggacag ccatttggtc gccatcaaag tccgcgttga aagcggaaca agccaaaggg
     5881 tgaagttgaa tcgcgttgcc ctcaatcaaa accggacgaa aagcctggat agaaagacgg
     5941 tgcaaagatg gcgcgcggtt tagaagaaca tatttgtttt taataacctc ttccaagatt
     6001 gcccaaacct ccggcgaacc ttcatcaatc aagcgtccgg cgccgcgaat attgtaggcc
     6061 aattcgcgtt ccaaaatttt agaaatgata aacggacgga aaagttccaa ggccatatgc
     6121 ttgggcaaac cgcactggtc aagttgaaga tccgggccaa caacaattac cgagcggcct
     6181 gaataatcca cacgcttacc caaaagattt tgacggaaac gaccctgctt gccctttagg
     6241 ttgtcggcca aagatttgag tggtcggcgt tgagcctgac tcatcgctcc accaccagaa
     6301 ccttgacgca ttgagttgtc caaaagcgag tctaccgctt cttgcaaaat gcgtttttcg
     6361 ttacgtaaaa taacttccgg cgcgtttagt tcaaccaatt tcttcaagcg attgttgcga
     6421 ttaatgaccc gacggtaaag atcgttcaca tcagaagtgg catgacgtcc gccatcaagc
     6481 gccaccatcg gtcgtaaagc cggcgggata accggcacca actttaggaa catccactcc
     6541 ggacgaacgc cagagcgacg cattgcccga tagagattca gtcttttctg caatttaacc
     6601 ctttcaaccg aactggcctt ggtcaagttc ccagttaggt tgttttccat ttcttccaaa
     6661 tctagggttc gcaaaatttc ataaatcgct tccgcgccaa tcccagcctc aaaaaccgtg
     6721 ccatacttga gcgacagacg gtgataaagg acttcgtcaa tcacccgcca aggcgtaata
     6781 ttctccaatt gacgcttggc ttccataaaa gcattcttca gattttcttt gtcttcttca
     6841 ttagttaaac cctttgattt ttggcgatat tcattatcaa gctctttgag cattttggac
     6901 ttctcgtcat cgttaacttt gatgatgatg tagccggcaa agtaaataac tttctccaga
     6961 tcggcaatcg tcaagtcaag cgccaaacca agtcgcgacg gcaccccgcg cacaaaccaa
     7021 atatgggcca ccggacaggc caactctata tgacccatgc gttcacggcg aacaattgat
     7081 ctagtcactt ctactccaca tttttcgcaa atgatatctt tgtaacgaat tcttttatac
     7141 ttgccacaat aacattcgta gtctttttcc ggaccaaaaa tccgttcatc aaacaaaccg
     7201 cttcgctcgg agcgtccact gcgataattg atggtttccg gtttggtcac ctcgccatgc
     7261 gaccattcca aaatcctctc cggagaagcc aacctcaggg taatcatatc gaagtcagtg
     7321 gctttgtctg acttgatgcc ggtttccagc gcggcgttgg tcattatttt attattcatg
     7381 gtcgtctttg ttattttttt taagttcaat gtccaacgcc aatccccgca gttcgttcaa
     7441 catcacgtta aacgaggccg gcaagtttgg ctgtttgata ctctcgcctt ttacaattga
     7501 gtcaaaagta gaagagcggc cggtaatgtc gtcagacttg atggtcaaca tttcacgcag
     7561 agtgtgagcc gcgccatacc cctccaaagc ccagacttcc atttcaccaa agcgctggcc
     7621 acccccttgc gagcgtccgc ccaaaggctg ttgggtaatg agcgagtaag gaccgatgga
     7681 gcgcatgtga atcttatctt ctaccatgtg cgccaatttg agcatataca tatagccaat
     7741 agcgatgtct ttttcaatct tctcgccggt ttgaccgtca aagagtgtta ctttgccctc
     7801 acgagaataa ccggcctttt gcaattcatc tttgatttcg ttttcggtcg cgcccataaa
     7861 aaccggggta atcgcttgat aacccagaga gtttgccgcc aaaccaaggt gcatttcaaa
     7921 aatctgacca aggttcatac gactaggcac gccgagcggg gtcaaaatga tatcaaccgg
     7981 cgttccgtca gccataaatg gcatatcttc ctccggtaaa atacgcgaga tgactccttt
     8041 gttgccatga cggccggcca acttgtcgcc caccgaaacg ttacgaattt gcgctatttg
     8101 aatttttatt tttttgataa taccggattc cagtttgtcg ccccgttctc gtgaaaaaac
     8161 ttttacgcca atcactctcc ccttgcgacc gtgcggcatc cgcaaagatg agtctttgac
     8221 atccctcgcc ttttcaccaa aaatggcgcg caaaagtctt tcctccggcg tcaattcagt
     8281 ttcgcccttc ggcgtaattt taccaacaag aatgtcattt tgttctacct cggcgccaat
     8341 gcgaacaatt ccgtcttcat ccaaattgcg aagtttaacc tcgccgacat tgggaatgtc
     8401 atgggttgtt acttccgggc caagtttggt gtcacggacg ttgacttcaa actcttcaat
     8461 gtagactgag gtaaatttgc tgtttttgac caaacgttca gacaaaataa tcgcgtcttc
     8521 atagttggtg ccgccccaag acataaaggc gacaaaaaca ttttggccaa cagctgtttg
     8581 gccgttgtca gtggaagaag cgtctgctaa caaatcacct tttttaacct tttgaccgac
     8641 cgcgacagtt ggtcgttggt gaaagcaagt ataatcgttg gtcattttaa agttgactaa
     8701 gttgtagaca tcttccttgc catcttcacg tttgacaact atatggcgac catcagcccg
     8761 agaaactgtt ccgcttgatt gagccaaaat taaccggccg gagtcaaacg cggcacgcgc
     8821 ctcagctccg gttgccacca agggcacttc cgggataata caaggcacgg cctgtttctg
     8881 catgtttgac cccatcagcg cgcggttcgc gtcatcatgt tccaaaaatg ggatcattga
     8941 cgtggcaata gaataagctt ggttaggcgc gacatcaata aaatgcacgt cagttttttt
     9001 gtagagaccc ggcattcctt tctgtctggc ttccacatat tcactcaaaa gagaaccgtc
     9061 ctcggcataa ttggcgccgg cgtgaaccac cacgtatttt tcttcttcca aagcgttcag
     9121 ataaacgatc tcacccgtaa tcttgccttt ttccaccttg cgataaggcg cctcaataat
     9181 gccaaaatcg ttcactcgag cgtaagcgga caagtgcaaa atcaaaccga tgtttgggcc
     9241 ttccggcgtg tggatcgggc aaaccttgcc gtagtgggaa gagtgcacgt cgcgcacctc
     9301 caaaccggct cgttcacgag tcaaaccacc cggcccaagc gccgagagag tgcgcaagtg
     9361 ttccagttca gccaggacat tttcttggtt catgaattgc gacaactggt tagtggagaa
     9421 aaattccttc aacctggcct gtaacggacg aggactgata atttggatcg gcaaaagggt
     9481 agaagtatca acggttgaca ttcggtcctg gatatttcgc ttcatctgca tcatcccaat
     9541 tttcagtttg gcttggagca tttcaccaat acaacgaact cgacgaaaac ccaagtggtc
     9601 gatgtcgtct tcctcggcgc cttttgtggt gttcagggta atgatgtgag aaacaatttt
     9661 gacaatatca tcaaaagtca aaacacgctc ctcggtatct ttgtccacct ccttctttcc
     9721 aaaccgttca ttaaaacgaa cgcgaccaac caccgacaag tcatatttct cgcggtcaaa
     9781 catcccacca ataaattctc tggagttggc ggccgtcgcc aagtcgctat ctcgcaatcg
     9841 gcggtaaact tcaatataag attcagaggc cgttttcgcg tgatctttgg ccaacgtctt
     9901 ttcaataaaa gttgtatcaa tcttttggtc ggcaaaaacc ttgattagtt cttcgtcgga
     9961 aatctcgcga ccttctttgt cggcaaaaat ccgaagcaaa gaagtgaccg ggaatttacg
    10021 tttgcggtca attcgaacat aaataacatc gtcaatttca gtttctagtt ctacccaggc
    10081 accgcgcgcc ggaatgattt tggcgccaaa aaacttatga ccctttattt cgttggcggt
    10141 aaagaaaact ccaaaacttc gcgccaattg cggcacgatg acccgttcaa taccattgat
    10201 aataaaagtg ccatgagacg tcatgagcgg aaaatccacc aaaaagattt cctgttcttt
    10261 gtcttggcta attgtcttat tccttaaatt gacttttacc cgaagcggcg cgtcataaga
    10321 caaattgttg tctctggcat agtattcatc aaacttcggc tcatcaagtt ggaaaccggt
    10381 aaactccaac tcaaactttt tttccgagta atctttaatt ggagtaaatt ctttaaataa
    10441 ctccttgaga ccttcttcga tcaaccaacg gaaagacttc ttttggacct ccaccaggtc
    10501 aggcatctcc gccatcggtt tcttataacg cgaaaaatat ttttgcttca ttaatttcgc
    10561 caaaaattaa aatcccctaa taaataaaaa cgcaaaacca ttccctcccc cttcttgagt
    10621 cgggtaattg atatgaaaaa tagatagttg tagcaagaag ttcaccaaaa actcaatctt
    10681 gcttgcgtac acaatagagt acactaacgg acttaaaatg tcaaactcaa tttctgtcaa
    10741 ggttataaaa ctggggaaaa ctgcctatct ccaattatat taggttagcg tttggcccgt
    10801 gtcttcctcc attcctccat cttcttgtgg tgcccggaca aaagcacttt tggcgcgcgg
    10861 taatttttgt ctccgtgctt gtaaacttcc ggcctggtat agacctcaga ggcggcaatt
    10921 cttttttctt caagtgacac ctcattgccc aacacgcccg gaattcgtct agccaccgcg
    10981 tccatcatca ccaaggctgg cagttcgccg ccagtcaaaa cgtaaggacc gatggaaatt
    11041 tcttccgcgc gtaaaatttt tttgactcgc gcgtcaatcc cctcatagtg accgcaaatg
    11101 ataatcagat gcccaagttt ccgcgaccac tggtcagcta ctccgttgtc aaattgctta
    11161 ccggccgggc cggtaacgat tattttagag gtcaagttaa aagatttgga tttcaaatta
    11221 ggagtcttaa caaaccgggc aggtttgacc gaacgggcga tatttttttg tgccgctttg
    11281 acggccagca aaatcggctc tacttgcaag accatccccg gaccaccgcc ataaggccgg
    11341 ccatccacct tgcgatgttt gtctttggta taattacgcg gattataaaa tttgaattta
    11401 accttttttt ctttttgggc acgccaaagc attgagctgt tcaggtacga ttcaaagctt
    11461 tcggggaaaa ttgtaataat gtgaaaaacc atatagttag gtcaaaagtt ctaaaacctt
    11521 aaaaaaaata taaaaagaca aatgcattaa atcactgaaa agagaaaaag aaaagcccaa
    11581 acaaaaaacc gcccgaaggc ggttttttgt tcactaacta gatctgcttg aggtcttcca
    11641 tcgcttggtc aaccgacctg gaagcgctgc tggtggccat gttgttcgcg gcgcccaccg
    11701 gttcgttaat ttttaaatta acgcgagcgt taaacttcat gccgaccacg cggagcaaag
    11761 tgcggatcgc tttggcggtg ttgccttggc ggccgatgat tttgcccatg tcaacagggt
    11821 tgacttcaag cgtcatcaag acgcccattt cgtcaacttt tctgtcaacc ttcacatcct
    11881 cgggactatc aaccaatgat ttgacaacgt actcgaggaa ttcctgatcc ttatgcattt
    11941 cagccatagt attatctgtt agtatctaat gtcttttcga cctcttaatt taactctaat
    12001 aataaaccca aaacagcctt ttgtcaaaaa ccggcttcac ctaaaaaact gaaacacctc
    12061 actggcatga ccagtgaggt gttttttatt caaaaattta ttcagccgct tgctcttctt
    12121 caaccggggt ttcttcggtt gaggcctcgg gcaactcttc ggttttggtt tcttcggttg
    12181 gagtttccgt atttccttct tcggcgatcg gctcaatcgg cttctcggct tcagccgcct
    12241 tggccgcttt ctgggccgct tcggcttttt ctttagactc tttggcggct ctggcttttt
    12301 tgcctaactt tccggaagca acattttttt tggttccttt caccacgcct tttttaacca
    12361 ataaattatg catcgtatca gaaacttgcg cgcctttacc aagccaatct aaaattcggt
    12421 cagccttcaa agacggttcg ccatgacgag catcatagtt cccaaccact tcaatcacct
    12481 ttccagtctt ggcggccgcg tgagaatcaa caacaacggc ccggaatgac gggctgtttt
    12541 tgcggccaac acgctgtaat ctaatcatta acataacgaa aatagcttaa caaaaatcca
    12601 cccaaacgtc aacagaggat ttgggcctcg ccccttttgc ttgacttttt ggctgttttt
    12661 tgctataata tatagatagg atactaaagt accgtaaacg gaccccagac ccccccctac
    12721 gagacgtggg gtttttagtc cttgcattaa aatatttatc tctctacatc a
//
LOCUS       DAOOLO010000019        12031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_5889_length_12031_cov_6.627088, whole genome shotgun sequence.
ACCESSION   DAOOLO010000019 DAOOLO010000000
VERSION     DAOOLO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5889_length_12031_cov_6.627088"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLH96_01850"
     CDS             complement(<1..312)
                     /locus_tag="PLH96_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB60546.1"
                     /translation="MENDPTKIKILIVDDDKFLVDMYSLKFSESGYEVETAQSGDEAV
                     KKLEAGVKPDIYLVDVVMPVMDGFELVENIKQKFDGNRGLIVILSNLGQKEDVEKGLG
                     LG"
     gene            complement(328..1908)
                     /locus_tag="PLH96_01855"
     CDS             complement(328..1908)
                     /locus_tag="PLH96_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPB60547.1"
                     /translation="MDDYYNISLKKLDDKKIPFEVLKYVPLESALNYLFLPVAVKDGV
                     LEVGMVNPDDTEAKDALQFIASKTRMPFKIYLLSKKDFDLAVKSYEGLSSGDSAGVVG
                     DIQTDIREAEQTVAKFNKPKSEETTTSSANIIEEAPIAKIVTVIIQNALSGGASDIHI
                     EPTHTTLKVRFRVDGVLYTSLTLPMTVHEAMVARIKILSNLKLDEKRVPQDGRFSYSY
                     EGRKADFRISTFPTYWGEKIVMRILDPEKRKVSLDTVGMTPAQQEIVKRAIKRPYGLI
                     LITGPTGSGKTTTLYAMLNMLDREKNNVVSLEDPIEYDMPGVSQSQVRPEINYTFANG
                     LRSILRQDPDIVMVGEMRDKETARLAIQASLTGHLVLSTLHTNNSIGAIPRLIDMGVE
                     PYLLPPTLVLMMAQRLIPTTCEDAKVPEPADESIRMMIEKQFADLPEQYRKNIPPVDK
                     IYRVHKTATCPSGTKGRTGVFEFLEMDRELERIILKNPGENEIYRYARSKGFMTMKED
                     ALLKSFQGITPFEEVNKL"
     gene            complement(1964..2440)
                     /locus_tag="PLH96_01860"
     CDS             complement(1964..2440)
                     /locus_tag="PLH96_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60548.1"
                     /translation="MNTKKIVTYVALAVIILIAIFWVVKVFTGEEEPQSGVTMSPSLV
                     SDANQIALADDPFFTLLNGIKDIDLTNLELLSHPIFVNGLQDLSRPVSDRGRGRENPF
                     AQIGAGNLSLGSLDDQDSPTKGVFETATSSEAVVSPEGGSDVTTDDGETADEAAVE"
     gene            complement(2445..3062)
                     /locus_tag="PLH96_01865"
     CDS             complement(2445..3062)
                     /locus_tag="PLH96_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60549.1"
                     /translation="MTKLITPIILIVLSVVLVFYFGSAVLNGSDQPLLKTWLLNNSGQ
                     GLKALLAEKDRLDEVLGNARTLREKMAELERAEKNITAADREKLDSFIPDRIDTVDFV
                     YDINNIAARHGMILQDVNIKASDETKTEGLSSRELSVAEAAGADTVADMTMTFRVSGS
                     YKSLLGFLDDLSVSLRVADVTSLAFSVGETGINQYDIGLKTYWVK"
     gene            complement(3065..3691)
                     /locus_tag="PLH96_01870"
     CDS             complement(3065..3691)
                     /locus_tag="PLH96_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60550.1"
                     /translation="MNNKLATSFIPKQTIGTSLPERREPLGLVTLVAVIIFAIAALYF
                     AGIFVYRYYVYNQINRPCPTGGESTQSCGLRESLEREKKDFQYESLARLKRLDTKLKN
                     GSTVLANHQTLRPLFDKLSNVTAHNVQFNDFKFNKNVVEAKGVASSYEDIAYQSKIFT
                     SEVAKIGEERKITAFSFSNFDLDGKGNVIFNLTLTVDPSLLLFVKNSQ"
     gene            complement(3715..4857)
                     /gene="pilM"
                     /locus_tag="PLH96_01875"
     CDS             complement(3715..4857)
                     /gene="pilM"
                     /locus_tag="PLH96_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPB60551.1"
                     /translation="MGIFDKVLKIVKDSTGPKEKSAVAIDFGSSSVKVVQLRKEKGRA
                     ILETYGELTCGPYAELSVGQATSLPAEKMLELLRDLFREANVTAKVGAMAIPLRNSLL
                     VSVEMPEMEESKLAEMVPIEARKYVPLPISEVILDWRLMPKSSLDEREPALESADSRN
                     RKKSYGNKVEILIASIQKDIVRLYQDIAQKLGLVSDVFEIETFSSIRSVMPNDLSATV
                     ILDIGAGTSKIAIVDYGLVRLSHTIVKGSQDVTIAIAKSLSVPFAKAEEIKRKFGLLG
                     DVDNGELLKSVSPTVEYIFAEAGKVMLQYQKEHKRAVDKIILIGGGAMMKGVGDVARE
                     VIGLPVVMGAPFDKVEAPVFLSNVLKETGPEFAVAIGLALRKLEDL"
     gene            4869..5609
                     /locus_tag="PLH96_01880"
     CDS             4869..5609
                     /locus_tag="PLH96_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPB60552.1"
                     /translation="MRYNNYSTIKKQNSLMDKTYPHKHRLTRPNFIIKKLLSFIFPPR
                     CVRCGLLGVFLCDDCRPQLSKSGETAENGVIAAFDYHDSAVRKTIHHLKYHNIRELAE
                     PLAAEMYEVFLTVADDELIFNRGAGKIFVIPVPLHKKRRRERGFNQAEELAKYFCAPD
                     PKNFQLRTDLVFKIKNTKPQAQQKKRAERLQNLRGAFTASTKKKIAGRTVVIIDDVVT
                     TGATVREIKKLLLKNGARQVWAVVAAHG"
     gene            complement(5723..8275)
                     /locus_tag="PLH96_01885"
     CDS             complement(5723..8275)
                     /locus_tag="PLH96_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HPB60553.1"
                     /translation="MKTKIFKILKVKIAFLTIWFLLALPVKAITDSHLRIDSFLTDGV
                     FVVPAGVTSVTFEGWGAGGTNGTDGATNGAGGGAAYMKATVPVSEGQEYAIVVGQGNP
                     EANGNGGDSSVSLGETVYLLAKGGKGGGSNGGLGGLVSEGIGDTGFNGANGVRGAGSA
                     MKYGGSAAGDSEDAQGSLPGDFFGGAGFARKLYGAGGNALLGQINKGTEGIVRATYSI
                     TVEDFPVVKSRNWGRTKASSTNHPINMPLGIQEGELLIIAFSGPQGEHDIVSDDSVDW
                     VKLISTTSGSITGSIFYKIAEGNDTATVVSSANAGSAHISFRIAAAGIPTVSSFANTG
                     TNPNPPLHDTGAESNYLWLTAITRKGSSIFSNGVNGMPANYKDFLYLSGIGAVDPEIS
                     VAGRHLESHEEDPGSFTAISSPYVAFTLAVPYQKPLMVASSADDLTIREAQSKTFEIS
                     LDRKPDNDVIVDFDYDSDDLILNKDTLTFTTLNWMDPQTVTVTALNDGLEIQERQANI
                     TLLVSSLDNNYNNFEAEDITVTIQNNRAATISIVAPLSGAVVSEKIEIQINVDDDYET
                     LGVQLKLDDQVWGPEYPPDSWPLLWDTAEIADGDYLLTAVARDDGGSYSTSSAVALTV
                     RNDVLARQISGGGRIRKKEKLQAPLPVSSTTQQTIMAYPRDLKLGLLGDDVLRLQVFL
                     NGQGFPVASTGPGSSGQETTYFGPATQAALIRYQQANKIVPAVGYFGSITQTQVYLTE
                     AGLSKSDPDLRLIGSMSGSSSPHHFATTLFFGLNHPDVRELQQFLNNDPDTLITKTGP
                     GSPNQETLYFGQLTLDAVRRFQRKYNLARPQDPGYGRVGPLTRAKLNELAGW"
     gene            8711..9691
                     /locus_tag="PLH96_01890"
     CDS             8711..9691
                     /locus_tag="PLH96_01890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB60554.1"
                     /translation="MPKKTFTIRKYKPGDEKKLINVFNEVFIENSGSHHQIMDKKIWD
                     WKFANSPFGFKILVAINNKKEIVGQLAIQKNRFKYRNRYSNFYSIVDLCLKKEYRNHN
                     FVQRCYERFLSKMNNVIIGFPSAVWAHSYPTIDPKIKVYHVPIFGKKFDKKSQKQYQD
                     ISGEISVERIRRISQAKNDLDKLWQKKKAEITIGCVRNARYLNWRFLKNPAPLNLYLI
                     KQGNNPIGYFVLEPKKTITLVKDILILNDQLKEGLKALTKINKDLPKSEIRLMTTDPT
                     LQKGLLKAGYAEYRREKINQNGYFVSHNPEKTELNEYYLTWADSDWDLYH"
     gene            complement(10117..10944)
                     /locus_tag="PLH96_01895"
     CDS             complement(10117..10944)
                     /locus_tag="PLH96_01895"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPB60555.1"
                     /translation="MILTEPSLPKSRLSLPVGLSRSTLYYHPKQPEKDWSLKCQIEEA
                     LHLHPSYGHKRLAIELKRNKKAVLRVMKLYGLQPYRRRGKKPFKINKPSGCIYPNLLL
                     TNCPSYEHHISAADFTYIPFQGKFVYLATVMDLFTRKIVGYAVLTNHSSQLVINALLS
                     ALLHSPRPLIFHSDNGTEYEAKDFTTLLASVGILISRSRPGSPWENGYQESFYSQFKV
                     DLGDPNRFETLGELVYEIYRLVWDYNHTRIHSAFKMAPAKFAAAQKRDTMELMQRVS"
     gene            complement(10941..11198)
                     /locus_tag="PLH96_01900"
     CDS             complement(10941..11198)
                     /locus_tag="PLH96_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB60556.1"
                     /translation="MTKTYRIAPEVKAQVINRIKNEGISVGLAAQEHGLSERTIYSWL
                     GRQAKGQPTWLEVTKLKKENQALLALVGELTIKLSNIQKKR"
     gene            complement(11247..11855)
                     /locus_tag="PLH96_01905"
     CDS             complement(11247..11855)
                     /locus_tag="PLH96_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60557.1"
                     /translation="MMIAVFTEDGILLMMDNWISFLREWQTLIGSALGPFLAIILTAI
                     GALIKSWIDKSKDRAEAMRRIEINSARALNDVFFIREKLKFFVGQLRKLANDVRATTN
                     PKEFSLHTVNCPVFGGAYIDRDLLNFRVHSYYLHNRAMWMDAELKEMNGILINLEKDF
                     ENVVKRNELLVGLMKDNETRDASVQRISYAENLDILWPVPQI"
BASE COUNT         3390 a         2854 c         2441 g         3346 t
ORIGIN      
        1 gccaagaccc aaacctttct caacatcctc tttttggcct aagttggaca aaataacaat
       61 caagccccga ttgccatcaa atttttgctt gatattttcc accaattcaa aaccgtccat
      121 gacgggcatc accacatcca ccagataaat atccggcttt accccggcct ctaatttttt
      181 gacggcctca tcaccgcttt gcgccgtctc tacctcgtaa ccgctttcac tgaatttaag
      241 tgaatacatg tccaccaaga atttgtcatc gtccacaatc agaattttta ttttagttgg
      301 gtcattttcc atattagttt aattatatta caatttgttg acttcttcaa aaggcgtaat
      361 cccctgaaat gatttgagaa gggcatcttc tttcattgtc ataaaacctt tggacctggc
      421 gtaacggtaa atctcatttt cgcccggatt ttttaagata atccgctcca gttcgcggtc
      481 catttccaaa aattcaaaga caccggtccg acccttggtg ccgctagggc aagtggcagt
      541 tttgtgaacg cgataaatct tatccaccgg cgggatgttt ttgcgatatt gctccggcaa
      601 gtcggcaaac tgtttctcaa tcatcattct gatactctca tcagccggtt ccggcacttt
      661 ggcgtcttcg caagtggttg gtatcaaacg ctgcgccatc atcaaaacaa gcgtcggcgg
      721 caatagatat ggttctaccc ccatatctat caaacgcgga atagcgccga tggaattgtt
      781 ggtgtgcaaa gtagaaagca ccaagtgacc agtcaaggaa gcctgaattg ccaaccgcgc
      841 cgtttctttg tctcgcattt caccaaccat cacaatgtcc gggtcctgac gcaagatact
      901 gcgcaaaccg ttggcaaagg tataattgat ttccggcctg acttgcgact gactaacccc
      961 cggcatgtcg tattcaatcg ggtcttcaag gctaacaaca ttattctttt cgcggtcaag
     1021 catgttaagc atcgcgtaaa gagtagttgt tttaccggaa ccagttggac cagtgatcaa
     1081 aattaaacca taagggcgct tgattgctcg tttgacaatc tcctgctgcg ccggcgtcat
     1141 tccgacggtg tccagactaa ccttgcgctt ctccgggtca agaatacgca tcacgatttt
     1201 ttccccccaa taagtcggaa aggtggagat acgaaaatcg gctttgcggc cttcgtaact
     1261 gtaagaaaat cgtccgtctt gcggcacgcg cttttcgtcc aatttgaggt tgctcaaaat
     1321 tttaatgcgc gccaccatcg cctcatggac agtcatcggt agtgttaggc tggtataaag
     1381 cacgccatcc acccgaaaac gaaccttaag ggtggtatgg gttggctcaa tatgaatgtc
     1441 agacgcgccg ccggaaaggg cattttggat aataacggtt acaatcttgg cgatcggcgc
     1501 ttcttcaatg atattagccg agcttgtagt cgtctcttcc gacttcggtt tgttaaactt
     1561 ggcgactgtt tgttcagctt cacggatgtc ggtctgaata tcgccgacca cccccgccga
     1621 gtctccgcta gacaaaccct cataactttt tacggccaaa tcaaaatctt tttttgacaa
     1681 aagataaatt ttgaatggca ttcgggtctt ggaggcaata aactgcaaag cgtctttcgc
     1741 ttccgtgtca tccggattaa ccatccccac ctctaaaaca ccgtctttga ctgccactgg
     1801 caaaaacaaa taattaagcg ccgattccag cggcacgtat tttagaacct caaaaggaat
     1861 ttttttatca tccagcttct tgagcgatat gttatagtag tcgtccattt taaaatttac
     1921 ttcccaaaaa ctgccgggaa taaaaagaag cggtttccct catctattcc actgcggcct
     1981 catcggccgt ctctccgtca tctgtcgtga catctgaacc cccttcgggc gaaacaacag
     2041 cctcgcttga agtggcggtt tcaaagactc ctttggttgg agaatcttgg tcgtccaaac
     2101 tgcccaaact caagttccca gctccaattt gcgcgaaagg attttcgcgg ccgcggccgc
     2161 ggtcggatac cggccgactc aaatcttgga gaccgtttac aaagattgga tgggagagca
     2221 gttctaggtt ggtcaaatca atatctttaa taccatttaa caaagtaaag aacgggtcat
     2281 ccgccaaagc aatttggtta gcgtcgctga ccaagctggg tgacatagtg acgccacttt
     2341 gcggttcttc ctcgccggta aaaaccttga ccacccaaaa aatggcgata agaatgataa
     2401 ctgccagggc gacgtaagta actatttttt tggtgttcat tttattattt tacccagtaa
     2461 gtttttaatc caatgtcata ttggttgatg ccggtttcgc cgaccgaaaa ggccaatgag
     2521 gtaacatcag ccactcgcag actgacagac aaatcatcca agaaaccaag cagagacttg
     2581 tagctgcccg agactctaaa agtcatggtc atatcagcaa ccgtatcggc gccagccgct
     2641 tcagcgacag acaattcgcg cgaactcaag ccttcggtct tggtttcgtc cgaagctttg
     2701 atattcacat cttgcaatat catgccatgc ctagcggcga tattgtttat gtcataaaca
     2761 aagtctactg tgtcaattcg gtcaggaata aaagagtcta acttctcgcg atcagcggcc
     2821 gtgatatttt tttcggcgcg ttccaactcc gccatttttt cgcgtaacgt tcgcgcgttg
     2881 ccgagaactt cgtcaagtct atctttttcg gccagtaaag ccttcagacc ctgaccactg
     2941 ttgtttaaga gccaagtttt tagtaatggc tggtcgcttc cgttcaaaac ggccgaacca
     3001 aaataaaaca ccagaacaac agacaagacg attagaataa ttggcgtaat tagtttggtc
     3061 ataattattg gctatttttg acaaataata ataaagaggg gtcgaccgtc aaagtcaggt
     3121 taaaaatgac gttacccttg ccgtctaaat caaagttgga aaatgaaaaa gcggtaattt
     3181 tgcgttcttc accgattttg gcaacttctg atgtaaaaat ttttgattgg taagcgatat
     3241 cttcgtaact ggaagcaacc ccctttgctt ctacaacgtt tttgttaaat ttaaaatcgt
     3301 taaactgcac attgtgggcc gtcacgttac tcaatttatc aaacaagggc cggagcgtct
     3361 ggtgattagc caatacggtg gagccgtttt ttagcttggt atccaaacgc tttagccgcg
     3421 ccaaagattc atattgaaaa tccttttttt cacgttctag agattcacgc aagccgcaac
     3481 tttgagtgct ttcgccgccg gtcggacacg gacgattaat ttgattgtaa acataatagc
     3541 gatagacaaa aataccggca aaataaagag cggctatcgc aaaaataatt accgccacca
     3601 atgtaaccaa gcccaggggt tcccgccttt ccggcaagct ggtaccaatg gtttgtttgg
     3661 gaataaatga ggttgctaat ttgttattca taatttttta ggttagatat tgacctaaag
     3721 gtcctctagt ttgcgcaaag ccaagccaat ggcaaccgca aattccggac ccgtttcctt
     3781 taatacgtta cttaaaaaga ctggggcttc aactttatca aacggcgccc ccatcaccac
     3841 cggcaaaccg attacctcgc gcgccacatc gccaactcct ttcatcatcg cgccaccacc
     3901 aatcaagata attttatcaa ccgcgcgctt gtgttccttc tgatattgta acattacctt
     3961 gccagcttca gcaaaaatgt attccacagt cggactgaca ctttttagca attcgccgtt
     4021 atcaacgtca ccaagcaagc caaattttct ttttatctct tcggctttgg caaatggcac
     4081 agacaaagac ttggcaatgg cgatggtaac gtcttgggaa cctttgacaa tcgtgtggga
     4141 cagacgcacc aagccgtaat ccacaatcgc gattttagaa gtgccggcgc caatgtccaa
     4201 aatcaccgtc gccgataggt cgttgggcat taccgaacga atagagctaa aggtttctat
     4261 ctcaaaaaca tccgagacca aacccagttt ttgagcgata tcttgataaa gtctgacgat
     4321 gtccttttgg atagaagcaa ttagaatttc taccttattg ccgtaacttt ttttgcgatt
     4381 gcgagaatca gcagactcta gagctggttc tcgttcatca agcgagcttt tgggcatcaa
     4441 ccgccaatca agtatcacct ccgaaatcgg caagggaaca tatttgcgcg cttcaatggg
     4501 caccatttcg gccaatttgg attcttccat ttccggcatc tctacactga ccaaaaggct
     4561 gttgcgaagc gggattgcca tcgctccgac cttggccgtg acgttggctt cgcgaaagag
     4621 gtcacgcaaa agctctaaca ttttttcggc cggtaaactt gtcgcctggc caactgaaag
     4681 ctcggcatag ggaccgcagg tcaactcgcc ataggtctcc aaaatggcgc ggcctttttc
     4741 cttgcgcaac tggacgactt tgaccgaaga agaaccaaaa tcaatcgcca ccgcgctctt
     4801 ctcctttggg cctgtgctgt cttttacaat ttttaaaact ttatcaaaaa tccccataaa
     4861 attttgaaat gagatacaat aattatagca cgattaaaaa acaaaactct ctcatggaca
     4921 agacttatcc acacaaacac cgcctgactc ggccgaactt cattattaag aaattgttat
     4981 ctttcatttt cccgccacgc tgcgttcgtt gcggtttgtt gggcgtcttt ttgtgcgacg
     5041 attgtcggcc ccaactatcc aaaagcggcg aaacggccga gaacggagtc attgccgctt
     5101 ttgactacca tgattcagct gtccgcaaaa ccattcatca tctcaaatat cataatatcc
     5161 gagaactggc cgagcccttg gctgccgaaa tgtatgaggt ctttttaacc gtggccgatg
     5221 atgagcttat ctttaaccgt ggtgccggca aaatctttgt gattcccgtc cctttacaca
     5281 aaaaacgccg ccgtgaacgc ggtttcaatc aagccgaaga actggccaaa tatttttgcg
     5341 cgcctgaccc taaaaatttc cagctaagaa ccgaccttgt ttttaaaatc aaaaacacca
     5401 aaccgcaagc ccaacagaaa aaacgcgccg agcgattaca aaatctaaga ggcgccttca
     5461 ccgccagcac caagaaaaaa attgccggcc gaacagtggt gattattgac gatgtcgtga
     5521 caaccggcgc tactgttaga gagataaaaa aattactgct taaaaacggc gcccgccaag
     5581 tttgggcggt ggtcgccgcg catgggtaag gccaggttaa aagtcaggag ctgaaaatgg
     5641 agaaaaaaat tgcaaattag attttgaagc agtttcccaa agttcagagt aattgagcaa
     5701 tttaagaact agattactct agttaccacc ccgccaactc attaagtttg gctctggtca
     5761 gagggccgac acgaccgtaa ccaggatctt ggggacgagc taaattatat ttccgctgaa
     5821 aacgtctgac tgcatctagg gttaattgac caaaataaag agtttcttga ttaggcgaac
     5881 ccggaccggt cttggtgatt aaagtgtcgg ggtcattatt caagaactgc tgaagttctc
     5941 gcacatcagg atggtttaac ccaaaaaaga gagtagtggc aaaatgatgg ggagaagatg
     6001 atccagacat tgatccaatt aaccttagat cggggtcaga tttgctcaat cccgcttcag
     6061 ttaggtaaac ttgagtttgc gtaattgaac caaaataacc aacggctggg acaattttgt
     6121 tggcttgctg gtagcggatc aaggctgctt gagtcgccgg tccgaaatag gttgtttctt
     6181 ggcctgacga gcccggaccg gttgaagcaa ccggaaaacc ttgaccatta agaaaaactt
     6241 ggagtcggag cacatcatcg cctaacaaac ctaatttgag gtcgcgagga taagccatga
     6301 ttgtctgctg agttgtagac gacacgggga gtggcgcttg aagtttttct tttttcctta
     6361 ttcttcctcc gccagatatc tgtcttgcca aaacatcatt acgcacagtc aacgcaaccg
     6421 cgctggaagt tgaataactt cccccgtcgt cgcgagcaac cgccgttaaa agataatccc
     6481 catcagctat ttcagcggta tcccataaaa gaggccaact atccggcgga tactccggac
     6541 cccagacttg atcatctagc ttcagttgca cacctaaggt ttcgtaatca tcgtcaacat
     6601 ttatttgaat ttcaattttc tcgctaacta ctgccccaga caaaggcgct actatagaaa
     6661 tggtggccgc ccgattgttt tgtattgtaa cggtaatatc ttctgcctca aaattattgt
     6721 agttattatc caaactggaa actaataaag tgatattagc ttggcgctcc tgaatttcca
     6781 acccgtcatt gagagccgtg accgttactg tttgtgggtc catccaattg agggtcgtaa
     6841 aagttaaagt gtctttattt aaaatcaaat catctgaatc atagtcaaaa tcaactatta
     6901 catcattatc gggtttcctg tctaagctga tttcaaaagt tttactttgg gcttctctaa
     6961 tcgttaaatc atcagcagag gaggcaacca ttaaaggttt ttgatacgga acggccaaag
     7021 taaaagccac atagggcgaa ctaatagctg taaaactccc cgggtcctcc tcgtgactct
     7081 ccaagtgtct accagctact gaaatttccg ggtctacagc cccaattccg gaaagataaa
     7141 gaaaatcttt gtaatttgcc ggcatgccat tgactccatt agaaaaaatt gatgaccctt
     7201 tccttgttat cgccgtcaac cagagataat tactctctgc tcccgtatca tgtaaggggg
     7261 gattcgggtt agtgccagtg tttgcaaaac tgctgacagt tggaatgcct gccgccgcaa
     7321 ttctaaaaga aatatgtgca ctcccagcgt tggcggaaga aacaaccgtc gctgtgtcgt
     7381 taccttccgc tattttataa aaaatagagc ctgtgatact accagaagtt gtagaaatta
     7441 attttaccca atctaccgaa tcatcagaaa cgatatcatg ctcaccttga gggccggaga
     7501 aagcgattat tagcaattcc ccctcctgaa ttcctaaggg catattgatt ggatgattag
     7561 tagaacttgc ttttgttcgt ccccaattac gcgatttgac tactgggaaa tcttctacag
     7621 taattgagta agtggccctg actattccct cagttccttt gtttatttgg cccaataaag
     7681 cgttaccgcc agcaccgtaa agttttcttg caaatccagc ccccccaaaa aaatccccag
     7741 ggagagaacc ttgagcgtct tcgctatcgc cagcggctga accaccatat ttcatggcgc
     7801 tacccgcgcc cctgacacca tttgctccat taaaaccggt atctcctatc ccttcactaa
     7861 ccaacccccc caagcctccg ttgctgcccc cgccttttcc acccttagcc aagagataaa
     7921 ccgtctcccc caagctgaca gagctgtcgc ccccgttgcc atttgcttcg gggttgcctt
     7981 gacccacaac aatggcatat tcctgacctt cagaaactgg aactgttgct ttcatatagg
     8041 cggcgccacc cccagcgccg tttgtggctc catccgtgcc attagtgcct ccggcccccc
     8101 agccctcaaa agtaaccgat gttactccgg cgggaacgac aaacacacca tccgttaaaa
     8161 acgaatctat ccgcaaatgt gaatcagtaa tcgctttaac cggcaaagcc aacaaaaacc
     8221 aaattgttag aaaagctatt ttgactttca gaattttaaa aatctttgtt ttcattttta
     8281 caaatttaac tataagtcat ccgaaattta aactactaaa ggctccgaac atttctttgt
     8341 cttgcttttg ccttctctat tatatcaact tttatccaaa tcactaatca aactcattag
     8401 atatcgaagt tcgtaaaggt aatttctttt ttcatattat tctaaatatc attaatctta
     8461 ttttactcaa aaacctgctt tagtataaca aaaatttagt acctattcca aatttagaaa
     8521 atttttttat ttgggcatta ggattgcctc atttcctccc cacatatcaa tgatagctgt
     8581 ctcgattgac tatctaaaaa atatttgatt tggttcatcg caaagacaat gagaaaaaag
     8641 ttttgccacc tctcccaatt atagagtgtt gaggtcatcc cctccatttt tttgttataa
     8701 tatttttaat atgcccaaaa aaacttttac catcagaaaa tacaaaccgg gagatgagaa
     8761 aaaactaatc aacgttttta acgaagtttt tattgaaaat agcggttctc accaccaaat
     8821 tatggataaa aaaatctggg actggaaatt tgccaattct cccttcggct ttaaaatttt
     8881 ggtggctata aataacaaaa aagaaatcgt cgggcaatta gcgatacaaa aaaatcgttt
     8941 taagtataga aaccgttatt ctaacttcta ttcaatcgtt gatttgtgct taaaaaaaga
     9001 gtatcgcaat cacaactttg ttcagcgttg ttatgagcga tttttgagca agatgaataa
     9061 tgtgattatt ggtttccctt ccgcggtctg ggctcactct tacccaacca tagaccccaa
     9121 aataaaagtt tatcacgtgc cgatatttgg caaaaaattt gacaaaaaat cacaaaaaca
     9181 atatcaggac atttctgggg agatttccgt agaacgtatc aggcgaatca gccaagcaaa
     9241 aaatgacctt gataaactgt ggcaaaaaaa gaaagcggaa ataacaattg gttgcgttag
     9301 aaacgcccga tatctaaact ggcgttttct aaaaaacccc gcccccctca atttatacct
     9361 aatcaaacaa ggcaataacc cgatcggcta ttttgtgctt gaacccaaaa agacaatcac
     9421 cttggtaaag gatatcctca ttttaaacga tcagcttaaa gaaggattga aagctttaac
     9481 aaaaataaac aaagatttac caaaatcaga aattcgttta atgacgactg accccaccct
     9541 tcaaaaaggc ttgcttaaag ctggctacgc cgagtatcgc cgagaaaaaa taaaccaaaa
     9601 tggctacttt gtcagccata atccggaaaa aaccgaactc aacgaatatt atttgacctg
     9661 ggccgacagc gactgggatt tatatcacta acgcttttta aaagctcttg ttgtgtcggc
     9721 acttcgtgcc gaccccattt cggcttcaaa atgcgatttc gatttctggc gggacactcg
     9781 ggttaagtgc taataaggtt agaccacata agggtattct agcatgtttg ggctaggtaa
     9841 aactaggcat gtgggacgga gctaaattta cacaaattac acatttttaa gccaattggc
     9901 ctctttaaat tacacacatt actcttcgtc atcgtcttca tcccgcatga tgctcgtatc
     9961 gacttttgac atatctgccc attttaccca tggctcgctc ttccttttgg gcgggacatg
    10021 aactctcttg atgggcatat attccgggct tcgtcggctc aaaaaatatc taattgcttt
    10081 cccttttcct ttcaagatgt gtagggtacc cctttcttaa gacactctct gcataagttc
    10141 cattgtatct cttttttggg cggcagcaaa tttagctggc gccattttga aggccgaatg
    10201 gattcttgta tggttgtaat cccagactaa gcggtaaatt tcatacacca attccccgag
    10261 tgtctcaaac cgattcgggt cacccagatc gaccttgaac tgcgaataga aagattcttg
    10321 gtagccattc tcccacggtg agcccggccg agagcgagaa atgagaatac cgacgctggc
    10381 cagtaaagtg gtgaaatctt tagcctcgta ctccgttcca ttgtcagaat ggaaaatcag
    10441 tggacgaggt gaatgtagca aagctgacag caaagcgttg ataaccaact ggctactgtg
    10501 gttagttaag acggcatagc ccacaatttt gcgggtaaac aaatccatca cggtggctaa
    10561 atagacaaat ttgccttgga atgggatgta ggtaaagtcg gccgccgaga tatgatgttc
    10621 gtaactcgga caattggtca gcaacaggtt gggatagata cagccactcg gcttgttgat
    10681 tttaaagggt ttcttgcctc gacgacgata tggctggaga ccatagagct tcatcactcg
    10741 caagacggct ttcttgtttc gcttcagttc aatggctaac cgtttgtgac catagctcgg
    10801 atgaaggtgc agcgcttctt caatctgaca tttcaaagac caatctttct ccggttgttt
    10861 agggtggtag tagagagtgg aacgagagag accgactggt agagacagtc gacttttagg
    10921 caacgatggt tcagtcagaa tcatcgtttt ttttggatat tagagagctt gatggttagt
    10981 tcgccaacta aagctagcag agcttggttt tctttcttga gcttggtgac ttccagccag
    11041 gtgggttggc ctttggcctg ccgacctaac caagaataga tggtacgctc actgaggcca
    11101 tgttcttggg cggctaaccc gacagaaata ccctcatttt tgatgcggtt aattacttga
    11161 gcctttacct ccggcgcaat acgatatgtt ttagtcatat acctgatttt acaaaattaa
    11221 gtgaataatt tcatgcaatc agtgtcttaa atttggggta ccggccacaa aatatctaaa
    11281 ttttcagcgt atgaaatgcg ctgaactgac gcatctcgtg tttcattatc cttcatgagc
    11341 ccaaccagaa gttcgtttct ttttaccaca ttttcaaaat ctttttcaag atttatcaat
    11401 attccattca tttcttttaa ttctgcatcc atccacatcg cccgattatg cagataataa
    11461 ctatgaaccc gaaaattgag taggtcacgg tcaatgtatg ccccgccaaa aacaggacaa
    11521 tttactgtgt gcaaagaaaa ttctttaggg ttagttgtag ctcgaacatc attagctaat
    11581 tttctaagtt gtccaacaaa gaatttcagt ttctcgcgta tgaaaaaaac atcatttaag
    11641 gctcgggctg agttgatttc tatgcgacgc attgcttcag ccctatcttt tgacttatcg
    11701 atccatgatt ttatcagtgc ccctatggca gtaaggataa tcgccaaaaa aggacccaac
    11761 gctgagccaa taagagtttg ccactctctc aaaaaactta tccagttatc catcattaaa
    11821 agtatgccat cttcagtaaa aacagcaatc atcaaaaatt agggcagtcg ggtcataccg
    11881 atacttagtt gtcaaggcgg ggaacaagcg attcgaacta agtgggtcgc ccatctgtca
    11941 taggtaatta ggtcataccg atacttagtt gtcaatgcgg agagggaggg attcgaaccc
    12001 tcgaggggcg ttaaccccta cctcgttagc a
//
LOCUS       DAOOLO010000020        11307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_6442_length_11307_cov_7.994046, whole genome shotgun sequence.
ACCESSION   DAOOLO010000020 DAOOLO010000000
VERSION     DAOOLO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_11307_cov_7.994046"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..169)
                     /locus_tag="PLH96_01910"
     tRNA            complement(82..169)
                     /locus_tag="PLH96_01910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(134..136),aa:Ser,seq:tga)
     gene            288..776
                     /locus_tag="PLH96_01915"
     CDS             288..776
                     /locus_tag="PLH96_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60558.1"
                     /translation="MEEKLKSLGLPAVIEWEADEYEERERSADWFWALGAVAFFGIVL
                     SFIFGNLLLALIIAIATFAIYAYENRPPQKMNIRLTPKGIQIGQDLYPYERADYFWID
                     EEREGLPMLIINYRRAFFPHLHISIEDVEPTLIRLYLRKFIEEEEHQKTFAEEISDLL
                     GF"
     gene            970..1041
                     /locus_tag="PLH96_01920"
     tRNA            970..1041
                     /locus_tag="PLH96_01920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1003..1005,aa:Arg,seq:gcg)
     gene            complement(1108..1374)
                     /gene="rpsT"
                     /locus_tag="PLH96_01925"
     CDS             complement(1108..1374)
                     /gene="rpsT"
                     /locus_tag="PLH96_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPB60559.1"
                     /translation="MPITKSAQKALRGSKQKKLFNDRRAKAMKSEIKEIKKLTEAKDA
                     KGASAKLSSAYQAIDKAAKRGVIKKNTAARKKSRLAKMIKKANV"
     gene            1441..2013
                     /gene="ruvA"
                     /locus_tag="PLH96_01930"
     CDS             1441..2013
                     /gene="ruvA"
                     /locus_tag="PLH96_01930"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPB60560.1"
                     /translation="MIGQLKGEISYKEERFVILNAGGVGYKVFVTPETGQNLKEKTTA
                     SLWTHLVVREDALDLYGFLAKTELQFFELLISVSGVGPKSALSVLSLAPPDVLKKAVS
                     AGDSSYLTQVSGIGRKIAEKIILELKDKVINIVGPDDNVDLTQETEALQALEALGYST
                     REAREALRRIPADIATTEDKVKKALKSLQK"
     gene            2020..3309
                     /gene="murE"
                     /locus_tag="PLH96_01935"
     CDS             2020..3309
                     /gene="murE"
                     /locus_tag="PLH96_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HPB60561.1"
                     /translation="MLDKILHWIKKFIPRRLFAKAQPFYHYLLAWLGAVIYRFPARRL
                     KVIFVTGTKGKTSTTEILSTILTAAGHKVASTSTLQFKIGDKIERNLYKMSMPGRMFM
                     QKFLRRAISAGCDFAVLEGTSEGAKLFRHKFIDCDALIFTNLSPEHLESHGSFEKYLD
                     AKLEYARALEKAKKTPRFLAVNSNDKKAKKFAELAPSVKLLEFSLTQAEPWETSASGS
                     KLTLANETLETSLTGEFNILNILGAATLAKAFGATNEDIKKALAELGKIPGRMEKVVS
                     DNSKQDFDVIVDYAHTADSLAKAYEALGNRRKICVLGSCGGGRDKWKRPQMGQVANDY
                     CDYIVLTNEDPYDEDPEQIIQEVAQGIAKPYVKILDRREAIRAALTEAKSGDVVIITG
                     KGTDPYIMGPNGKKTPWSDYEVAKEELSAKLCRPNAD"
     gene            3643..4509
                     /gene="mutM"
                     /locus_tag="PLH96_01940"
     CDS             3643..4509
                     /gene="mutM"
                     /locus_tag="PLH96_01940"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPB60562.1"
                     /translation="MPELPEVETLKRELSKVLPGRQIKKTEILWWKTVSPLSPEEFAE
                     QTIGKKIAGVNRRAKMLFIDLADSTALAIHLKMTGQLIFEPPKGRAVGGGHPDQALNK
                     KQPTKHTRLIFTFKDGSKLFFNDLRKFGWVRLMNDSQIKKLTAPIGLEPLSKIFTDQA
                     LLGIFHRYPNRTIKQTLLDQSLVAGLGNIYVDEACFGARLLPTRRNKNLKPTEIKKLR
                     QTIIAVLKLSLRHKGTSSKNYRRSDGSLGGFVPFLKVYGRQHEPCKTCGQPIHKITHA
                     GRGTHFCPSCQK"
     gene            complement(4512..4844)
                     /locus_tag="PLH96_01945"
     CDS             complement(4512..4844)
                     /locus_tag="PLH96_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPB60563.1"
                     /translation="MKIIINWVIAAIIILVAAYLVPGVAVSSFWVAFVAAVVFGVVNA
                     LIKPLVVILTLPINLITLGLFTLVINALLALLVAFVVPGFTIAGFWVALIFGIILTLI
                     NWAVERAF"
     gene            complement(4905..5393)
                     /locus_tag="PLH96_01950"
     CDS             complement(4905..5393)
                     /locus_tag="PLH96_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPB60564.1"
                     /translation="MALKKPQKEIVVVLPDIRSALNVGSILRTADAAGITKVWLAGYT
                     PAPMDKFGRVNKQINKTALGAEQAMPWEKAEDIQKLLRRLARAGFDLVAVEQATKAID
                     YKKFKPRTKTAFIFGNEAEGLPPKILRRCCQVIEIPMRGKKESLNVAVAAGIILFRTL
                     NI"
     gene            5591..5663
                     /locus_tag="PLH96_01955"
     tRNA            5591..5663
                     /locus_tag="PLH96_01955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5624..5626,aa:Lys,seq:ttt)
     gene            complement(5948..6712)
                     /locus_tag="PLH96_01960"
     CDS             complement(5948..6712)
                     /locus_tag="PLH96_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60565.1"
                     /translation="MLYLTYGSDKKKTRDKHLALAHSILRKQPEATFFKLSAENFSSS
                     EFEELIFGQTLFVRRFVVAGDNLLKEIKDKAAKEFILARLTDIAESENIFLFLEEEEL
                     LGKGASFEDKDKWKKRHQEIEKIKKVAVKAQEFSVLPPSEFNIYSIVDAFSARDKNEA
                     WTLYEEALLSGVSADEVLWKIIWQVQNMILVKKTKNEKELNLKPFVLNKTKRAAQKFE
                     VWELEKLSGELVDLYHETYLGSEEFEFGLEKILLGV"
     gene            complement(6773..7552)
                     /locus_tag="PLH96_01965"
     CDS             complement(6773..7552)
                     /locus_tag="PLH96_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003733676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPB60566.1"
                     /translation="MKLFSWNVNGIRAAVKSGFFEEFLAEVQPDVLGLQEIKISNSAR
                     EKAEFDFKIYNEYWHPAQKPGYAGTAVFAKVEPLAIGKGFVAPGKTNGNDEEGRVLTL
                     EFEKFFFVNTYFPNARHDLSRLTLKEDFNDRWLQHVKQLEKIKPVVTCGDFNVAHREI
                     DLARPKDNVGNPGFTNEERRWADRFAVAELVDTFRLVHGDQENIYSWWSYKFQARARN
                     IGWRIDYFWVSQSLRKNVKDAFILDTQLGSDHCPVGISLEI"
     gene            complement(7574..8176)
                     /locus_tag="PLH96_01970"
     CDS             complement(7574..8176)
                     /locus_tag="PLH96_01970"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB60567.1"
                     /translation="MTLKEKTEKLRVIKNELLGLEKSPLFSFRQANNFFPVIGEGNHE
                     AKIMFVGEAPGRNEAKTGRPFCGAAGKILDDLLTGVGINRAEVYVTNIIKDRPPQNRD
                     PLPEEIACYGPFLDRQIEIIEPRVIATLGRFSMRYILEKFAVPEALKSISELHGKIFS
                     AQAPYGPIVVIPFYHPAAAIYNRALIDDLKKDFAILKNYR"
     gene            complement(8182..8652)
                     /locus_tag="PLH96_01975"
     CDS             complement(8182..8652)
                     /locus_tag="PLH96_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPB60568.1"
                     /translation="MFEIAGGEIILSLGLAVILGVCLGFERNLAGKVAGMRTFALVSL
                     GSALFAVISKMVTEQTVGVINFDPLRMASQVVVGVGFLGAGLIMARTGKVTGLTTAAG
                     MWVAAGVGLACGFGFYWLALLAAGLTIFIFAVLWIIQRDFVSGLQKKVSGDDKG"
     gene            complement(8752..8961)
                     /locus_tag="PLH96_01980"
     CDS             complement(8752..8961)
                     /locus_tag="PLH96_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60569.1"
                     /translation="MKNYVIVAIIIILIIIGVVWYKSQTPATPEGEIETPVEETTDLE
                     GATGEVLEGEVEVVDEIATTSEVAE"
     assembly_gap    9040..9139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9140..11268)
                     /locus_tag="PLH96_01985"
     CDS             complement(<9140..11268)
                     /locus_tag="PLH96_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPB60570.1"
                     /translation="MTEKTKKLVLFDAHAIIHRAYHALPDFSSSQGEPTGGLYGVSTM
                     LMRIIADFKPDYLAACFDRAEATFRKQVYEGYKAGRPKAEEELIAQLIRARDIFAAFG
                     IPIYDAAGFEADDVIGTIVEKLKDEKNLQIIIASGDMDTLQLVRGNEVVVYTLRKGLT
                     DTVTYDEKAVRERYGFAPSLVADFKGLKGDPSDNIIGVPGIGDKTATTLIQEFGSVEN
                     IFEKLKQDEAVFADRGIKPRVVTLLKENEEEAIFSKTLATIRQDVPIDFSLPAQTWPE
                     TFSPAPVEKIFSELEFRSLIGRIKSLKTKDEPKDESKDLVETEFAPQDIRRVGVMVWL
                     LNSDLTVPTWEDIRQFTGCDSLLEAEKFALAELKKKKLDKVWTEIEAPLLPIIEKAEK
                     RGILVDANYLKNLAKDYHHKLAKLENEIYEVVGEIFNLNSPKQLGEVLFDKLGLSAKG
                     LKKTATGARSTRESELLKLKGTHPVIEKILAHRELQKLLSTYIDNIPSMLDNESALHT
                     TLNQNGTTTGRMSSNNPNLQNIPTRDSLGAAVRGAFVARPGFKLLAFDYSQIELRILA
                     VLSGDPSLAEVFKTGKDVHSSVAAKVFGVKEEVVTKEMRRRAKVINFGIIYGMGVNAL
                     KTSLGTTRQEAEQFMTNYFDTFPTIKKYFANVVALAKQRGYTETLFGRRRYLPALKSR
                     LPQILAGAERMAMNAPLQGTAADVIKLA"
BASE COUNT         3074 a         2767 c         2302 g         3064 t
ORIGIN      
        1 ctagttttct catatgtatt tgagtatata ctttagtata tactttagta tatactttag
       61 tatataccta ttatacacca ttggcggagg gtagaggact cgaacctcta agccccttgc
      121 ggggccccag ttttcaagac tggtgcctta ccattcggcg caaccctccc ggatcaatct
      181 cctcccaatc ttagcaaatt aagtcacaaa atcaacgaac accaaccaac ttgtttatca
      241 acaattacct gtattttttc tcaggcttaa ttgttataat gtagtcaatg gaagaaaaac
      301 tcaaaagtct gggtttgccg gcggtgattg aatgggaagc cgatgaatac gaagagcgcg
      361 agcggagcgc cgattggttt tgggcgcttg gcgcagtcgc cttttttggc attgtgctgt
      421 cttttatttt tggcaacctg cttttggcgc tcatcattgc gattgccact tttgccattt
      481 acgcttatga gaatcgcccg ccgcaaaaaa tgaatattcg tctgactccc aagggtatcc
      541 aaattggtca agacctttac ccttacgaac gcgctgatta tttttggatt gatgaagaac
      601 gcgagggttt gcccatgctt atcatcaact accggcgagc cttttttcct cacctgcata
      661 tttctattga agacgttgaa ccaactctga ttcgccttta cctccgcaaa tttattgaag
      721 aagaagagca ccagaaaact tttgccgagg agatttccga tcttttggga ttttaggttt
      781 tccttttatt acttctgaac ttttaacttt cattgggggc gaagtgattc gctttttttg
      841 taatttgtgg taaattaaat ctgcccatcc cctcacagtt taatggaccc cgtggttggg
      901 gcaacggggc gagaagtgga gttgaggtaa gtttagagat agttcatttg taatcaaaat
      961 atgtaaaatg cccctgtagt ttaatggata gaacgcagga ttgcggatcc tgtaatcgag
     1021 gttcgattcc tcgtgggggc acaaaaaaaa cgaaaagaaa caaatcaaaa accacctcgc
     1081 cttttggcga ggtggttttt gatttgttta aacgttagct tttttaatca ttttggccag
     1141 acgggatttc ttgcgcgcgg cggtgttttt cttgataact ccccttttgg ccgccttatc
     1201 aatcgcctgg taagccgaag acaacttggc gctggcgccc ttggcatctt tggcttctgt
     1261 cagttttttg atttctttga tttcgctctt catcgctttg gcgcgtcggt cgttgaacaa
     1321 tttcttctgt ttagaaccac gcaaagcttt ttgagctgat ttggtaattg gcataccgtc
     1381 acagaataca agaattacca aataattgca agtgttccgg cgacaaggta ttattgattt
     1441 atgattgggc aactcaaagg ggaaatcagc tacaaagaag agcgttttgt tattttaaac
     1501 gccggcggtg tcggctacaa agtatttgtt acgccggaaa ccggccaaaa cttaaaagaa
     1561 aaaacgaccg ccagtctttg gacccacttg gttgttcgcg aagacgcctt ggacctttac
     1621 ggctttctcg ccaaaaccga actgcaattt tttgaactcc tcatcagcgt ttctggcgtc
     1681 ggccccaaaa gcgctttgtc ggttttgtcg ttagctcccc cggacgtcct caaaaaggcc
     1741 gtctccgccg gtgattcttc ttatctgaca caagtttccg gcatcggacg caaaattgcc
     1801 gagaaaatca ttcttgaatt gaaagacaag gtcatcaaca ttgtcggtcc cgatgacaat
     1861 gttgacctca cccaagaaac tgaggcgctc caagcccttg aagcgctcgg ctactccact
     1921 cgcgaggctc gcgaggccct gcgccgaatc ccagccgaca ttgccacgac cgaagacaag
     1981 gtcaaaaaag cgctcaaaag tttgcaaaaa taaaaacaaa tgttggacaa aattttacat
     2041 tggataaaaa aatttattcc gcgccggctc tttgccaaag cccagccttt ttatcattat
     2101 ctgctggctt ggctaggcgc ggttatttac cgctttcccg ctcgtcgcct gaaagtcatt
     2161 tttgtgaccg gcaccaaagg caaaaccagc accacggaaa ttttgtccac tattctaacc
     2221 gccgccggac acaaggttgc ttctacttcc acgctgcaat ttaaaatcgg tgacaaaatt
     2281 gagcgcaacc tctacaaaat gagcatgccg ggacggatgt ttatgcaaaa atttctccgg
     2341 cgcgcgattt cggccggctg cgactttgcc gtgctggaag gcacttcaga aggcgccaaa
     2401 ctttttcgtc ataaatttat agattgcgat gctctgattt ttaccaacct ctcccccgaa
     2461 caccttgagt cgcacgggtc ttttgaaaaa tatctggacg ccaaattgga atacgcgcgc
     2521 gcgttggaaa aagcaaaaaa gacgccgaga tttttggcgg taaattctaa tgacaaaaag
     2581 gccaaaaaat ttgccgagtt ggcgccttca gtcaagctgc ttgaattttc cctaacgcaa
     2641 gccgaaccct gggagaccag cgcaagcggt tccaaactga ctctcgccaa tgaaacttta
     2701 gaaaccagct tgactggtga attcaatatc ttaaatattt tgggcgccgc caccctcgcc
     2761 aaagcttttg gcgcgaccaa tgaggatatc aagaaagcgt tggcggaact tggcaaaatt
     2821 cccggccgaa tggaaaaagt tgtttcggat aactccaaac aagattttga cgtcattgta
     2881 gactatgccc ataccgccga ctccctcgcc aaagcctacg aagccttggg caatcgccgt
     2941 aaaatttgtg tcttgggcag ctgcggcggc ggtcgcgata aatggaaacg tccacaaatg
     3001 ggccaggtcg ccaatgatta ttgcgattac attgttctca ctaacgaaga cccttacgac
     3061 gaggacccgg aacaaatcat tcaggaggtg gcccaaggaa ttgccaaacc ttatgttaaa
     3121 attttagacc gccgtgaagc gattcgcgcc gccctgacag aagccaaatc aggcgacgtg
     3181 gtcattatta ccggcaaagg caccgacccc tacatcatgg gccccaatgg caagaagaca
     3241 ccttggagcg actatgaagt ggctaaagaa gaattgagcg cgaaactatg caggccgaac
     3301 gcggactaaa atacagacaa cgcacttatc tacccatctc gccgtaattc atttaactat
     3361 taaacttttt ctgactgaca cccattctgc cttcgccaat ttaatggcta ggtccgtcct
     3421 agccaaaccc ttattttaag ccagtttcaa caggcgggtg tgttgagccg tttgtttttt
     3481 ctatctctac tcatattata atcactcctc ttattttaca ttttagtttt tccatcttac
     3541 actaccccta accttctttg ttttttgatt ttacacttta atttaaaacc tttttctttt
     3601 taacatttta ccttttaatc tttacatttt acattagcat ttatgcccga actccccgaa
     3661 gttgaaactc tcaaacgcga actgagcaaa gttctgcccg gtcgacaaat aaaaaagacg
     3721 gagattttgt ggtggaagac agtctcccct ctctcgcccg aagaatttgc cgaacaaacg
     3781 attggcaaaa aaattgccgg cgtcaatcgc cgcgccaaaa tgcttttcat cgatttggcc
     3841 gattctaccg cccttgccat tcacctaaaa atgaccggac aactgatttt tgaacccccc
     3901 aaagggcgag ccgttggcgg cggacatcct gaccaagcct tgaacaaaaa acagcccacc
     3961 aaacacacac gattaatttt tacttttaaa gatggttcca aattattttt taatgactta
     4021 cgcaaattcg gctgggttcg cttgatgaat gattctcaaa taaaaaaact gacagcacca
     4081 atcggtcttg aacccttgtc taaaattttt accgaccaag ctttgctggg catctttcac
     4141 cgttacccca accgcaccat caaacagacg ctcctggatc aatcgctggt ggccggattg
     4201 ggaaatattt atgttgatga agcttgtttc ggcgcgcgcc tcttgccgac caggcgcaac
     4261 aaaaacttaa agccgaccga aatcaaaaaa ctgcgacaaa ccattattgc cgtgcttaaa
     4321 ctttcccttc gccacaaagg cacttcgtct aaaaactatc gtcgttcaga cggttcgctt
     4381 ggcggctttg tcccctttct caaagtttat ggccgccagc acgaaccttg taaaacttgc
     4441 ggccaaccaa tccacaaaat cacccacgcc ggccgcggca ctcacttttg cccaagttgc
     4501 caaaaataat tttaaaaagc tctctcaacc gcccagttaa ttaaagtcaa aataatccca
     4561 aaaataagag ccacccaaaa gccggcaatc gtaaaaccag gcaccacaaa cgccaccaag
     4621 agagccagca gggcattgat taccaacgta aaaaggccca gagtgataag attgatgggg
     4681 agagtcaaaa tgactaccag cggcttaatg agcgcgttga caaccccaaa aacaacggcc
     4741 gccacaaacg ctacccaaaa actgctgacc gccacgccgg gcaccaagta ggcagcaacg
     4801 agaatgataa tggccgcaat aacccagttg atgataattt tcatggtttt ttaggttaac
     4861 tttaataaat ttataaaaac aaggttgttt aaagaagcga ttaatcaaat attcagagtt
     4921 ctgaagagga taatgccggc cgccaccgcc acattaagag actccttttt gcctcgcatc
     4981 ggaatctcaa tcacctgaca acatcgcctt aaaatctttg ggggcaaacc ctcagcctcg
     5041 ttgccaaaga taaaagcagt ttttgttcgg ggtttaaact ttttgtaatc aattgccttg
     5101 gttgcttgtt caaccgccac caggtcaaag cccgcgcgcg ccaaccggcg caaaagtttt
     5161 tggatatctt cggctttttc ccaaggcatg gcttgttccg cgcccaaggc cgtcttgttt
     5221 atttgcttgt ttactcggcc aaatttgtcc attggcgccg gcgtataacc agccaaccag
     5281 acttttgtta tccccgcggc atcagcagtt cgcaaaatag aaccgacatt tagagcgctc
     5341 cgaatatccg gcaaaacaac aactatctct ttttggggtt ttttaagcgc cattaatagc
     5401 aatataacac agccaaaaga tttgaaaaac tcaaaaaatc tgttattatt tatgcgcgac
     5461 acaaatatac gaatggaacg aatttaatat gccctgttag ctcagccccg acgctcagag
     5521 ggtcgggacg agtaagttac gtaaagtttg tgattaagta aagtaagtat tgttatgtaa
     5581 attatcatta gccctgttag ctcagttggt agagcagctc ccttttaagg agacggtcgg
     5641 gggttcgaat ccctcacagg gcacattaaa aaaatcggct ttaggccgat ttttttaatg
     5701 tgccctgtag caagtgaatt gattcacttg tgttagggat tcgaagaccg cagcgatgcc
     5761 gagccaacag gcgaggccgc gaggggcggc ctgcgagttt gccgagtggc tgacgaggca
     5821 aaacttgtag gcgaatcctg ggcacaaaat tacgaagaaa aatgtgcgag catttttcga
     5881 ctattgtttt gtagccctgt gagggatttg ttacgtagcc tgttacgtaa caagctacgt
     5941 aacaaatcta tactcccaat agaatcttct ccaaaccaaa ctcaaattct tccgagccca
     6001 aataagtttc gtgatagagg tcaaccagtt cacctgataa tttttctaac tcccacacct
     6061 caaacttctg cgcggctcgt ttagttttgt ttaaaacaaa tggttttaag tttaattctt
     6121 tttcgttttt ggtttttttc accaaaatca tgttttgcac ttgccagatt attttccaca
     6181 agacctcgtc tgccgagaca ccggacaaga gcgcctcttc ataaagagtc caggcttcat
     6241 ttttatcgcg cgccgaaaaa gcatcaacga ttgaataaat gttaaattcc gacggcggca
     6301 aaacggaaaa ttcctgagcc ttaaccgcca ccttcttaat tttttctatc tcctgatgtc
     6361 tttttttcca tttatctttg tcttcaaaag aagcgccttt acccaacaat tcctcttctt
     6421 ctaaaaacag aaagatgttt tctgattccg cgatgtcggt tagacgcgct aaaataaatt
     6481 ctttggccgc tttatctttt atctctttca ataagttatc accagcgacc acaaaacggc
     6541 gaacaaaaag tgtttgacca aatattaact cttcaaattc ggaacttgaa aagttttctg
     6601 cgcttaattt aaaaaaggtt gcttcgggtt gtttgcgcaa aattgaatgg gccaaagcca
     6661 ggtgtttgtc tcgtgtcttt tttttatcag aaccataagt aaggtagagc ataggataat
     6721 gtaaaattta aaaattaaaa tcgaagataa aaagttctaa taattttaca ttttaaattt
     6781 caaggctgat gccgaccgga caatggtcag aaccaagctg cgtatccaaa ataaacgcgt
     6841 ctttgacatt tttccggagt gactgactta cccaaaaata gtctatccgc cagccgatgt
     6901 tcctggcgcg cgcttggaac ttataactcc accatgaata gatgttttct tggtcgccat
     6961 gaactagccg gaaggtatcc accaattccg ccaccgcgaa tctgtcggcc caacggcgct
     7021 cttcgttggt aaaaccaggg ttgccaacat tgtctttggg gcgagccaaa tcaatttccc
     7081 gatgcgcgac attaaaatcg ccgcaagtaa ccaccggttt tattttttct aactgcttca
     7141 cgtgctggag ccagcggtca ttaaaatctt cttttaaagt caggcgcgac aagtcgtggc
     7201 gcgcgttcgg aaagtaggtg ttaacaaaaa agaatttttc aaactccaag gtcaacaccc
     7261 gaccctcttc atcgttccca ttggttttgc cgggcgcgac aaaaccctta ccgattgcca
     7321 gcggctctac cttggcaaaa accgccgtgc cggcgtagcc cggtttctgg gccggatgcc
     7381 aatattcgtt ataaatctta aaatcaaatt ccgccttttc tctcgcgctg ttagaaattt
     7441 ttatttcctg caaacccaaa acgtcaggct ggacttcggc caaaaactct tcaaaaaaac
     7501 cgctttttac ggccgcgcga atgccgttta cattccaaga aaatagtttc agaggcttag
     7561 ccataatcta atcttagcgg taattcttaa gaatggcaaa gtcttttttt aaatcatcaa
     7621 tcagggctcg attataaatc gccgcggctg gatgataaaa cggaataacc acaattggcc
     7681 catagggagc ctgggctgaa aaaattttgc cgtgaagttc gctgattgac ttcaacgctt
     7741 ccggcacagc aaatttttcc aaaatatagc gcatggaaaa acgacccaga gtggcgataa
     7801 ctcgcggctc aataatctca atttgccggt caaggaacgg gccataacag gcgatctctt
     7861 ccggcaatgg gtcccgattt tgtggcggtc ggtctttgat aatattggtc acgtaaactt
     7921 ccgcgcggtt aattccgaca ccggtcaaca aatcgtccaa aattttgccg gccgcgccgc
     7981 aaaaaggtcg ccccgtcttg gcttcgttac gtcccggcgc ttcaccgaca aacataattt
     8041 tggcttcgtg atttccctcg ccaataaccg gaaaaaaatt atttgcttgt cgaaaagaaa
     8101 atagcggtga tttttcaagc cccaaaagct cgtttttaat gactcgcaat ttttccgttt
     8161 tttctttcag ggtcatagcc tttagccctt atcatcgcca ctgacttttt tttgcagacc
     8221 gctcacaaaa tcacgttgaa ttatccaaag aacggcaaag ataaaaatcg tcagaccggc
     8281 ggcaagcaac gccagccagt aaaaaccaaa gccgcaagcc aagccaacgc cagcggccac
     8341 ccacattccg gccgcagttg tcagccccgt caccttgccg gttctggcca taatcaaacc
     8401 ggcccctaga aaaccgactc ccactaccac ctgcgaggcc atccgcaagg ggtcaaagtt
     8461 tatcactcca actgtctgct cggtgaccat tttggagata accgcaaaaa gcgccgaccc
     8521 caaactaacc aaggcgaagg ttctcatccc cgctaccttg cctgccaagt tgcgttcgaa
     8581 accaaggcag acacctaaaa taaccgccaa ccccaaactt aaaattattt ccccgcccgc
     8641 tatttcaaac ataaaaaatt aaaattactg ctatgtctct attttaaaat tttttacgaa
     8701 aaaaaacaaa aaaaccgcca gtggcggttt ttttgttttt ttgactgtaa attattcagc
     8761 aacttcggaa gtggtggcaa tttcgtcaac aacctcaacc tcgccttcca aaacttcccc
     8821 cgtggcgcct tctaagtcgg tcgtttcttc aactggagtt tcaatctcgc cttccggagt
     8881 ggccggagtt tgagacttgt accaaactac gccaatgata atcaagatga ttataatagc
     8941 gacaataacg taatttttca tggattatag tgtttgtttt actttaatcg acctttgctt
     9001 gattgcaaat tgtaacattg cgatttcatc atatagtatn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnng ccagcttgat tacatcggcg gccgttcctt ggagcggcgc
     9181 gttcatcgcc attctttcgg cccccgccaa gatttgcggc aaacgcgatt tgagcgccgg
     9241 caaataacga cggcgcccaa acaaggtttc ggtataaccc ctctgcttgg ccagggccac
     9301 cacatttgcg aaatactttt ttattgttgg aaaagtgtca aaatagttag tcatgaattg
     9361 ttccgcctct tgcctggtgg tacccagact ggttttcaga gcatttacac ccataccata
     9421 aattatgccg aagtttatga ctttggcgcg gcggcgcatt tctttggtaa ccacctcttc
     9481 ttttacgcca aaaactttag cggcgaccga tgaatggaca tctttgccag ttttaaacac
     9541 ctccgccaaa ctggggtcgc ccgataaaac cgccaaaata cgaagctcaa tctgcgagta
     9601 gtcaaaggcc agaagcttaa agccagggcg agccacaaaa gcgccccgca cagccgcccc
     9661 tagactgtct cgggtcggaa tattttgcaa attcgggttg ttggaagaca tccgaccggt
     9721 cgtggtgcca ttttggttga gagtagtatg aagcgcgctt tcgttatcaa gcatggatgg
     9781 aatattgtct atgtaagtgg agagcagttt ttgtaattca cgatgcgcca aaatcttttc
     9841 aataaccgga tgggtccctt ttagttttag gagttccgat tcgcgagtgg accgcgcgcc
     9901 ggtggccgtc ttttttagac ccttggcgga aagccccagt ttgtcaaaca acacttcgcc
     9961 caattgtttt ggcgaattaa gattaaaaat ttcaccgaca acttcataaa tttcattttc
    10021 cagcttggct aacttgtgat ggtaatcttt ggccaggttt ttcaaataat ttgcgtcaac
    10081 caaaatacct cgtttttcgg ctttttcaat tatcggcaaa agcggcgcct caatctcggt
    10141 ccaaacttta tctaactttt tctttttcag ttcggccaaa gcgaattttt ccgcttcaag
    10201 caaactatca caaccggtaa attgtcggat atcttcccaa gtcggtaccg tcaaatcaga
    10261 attaagaagc caaaccatca ctcccactcg acgaatatct tggggggcaa attcagtttc
    10321 gaccaagtct tttgattcgt cttttggttc atctttggtt tttaaactct ttattcgccc
    10381 aatcaagcta cgaaactcca actcggaaaa aatcttttcc actggcgcgg gcgagaaagt
    10441 ttccggccaa gtttgcgccg gcaaagaaaa atcaattggc acatcctggc gaattgtcgc
    10501 cagggttttg gaaaaaatcg cttcttcttc gttttctttc aacaaagtga cgacccgcgg
    10561 cttaatcccc ctgtcggcaa aaaccgcttc gtcttgtttt agtttttcaa aaatattctc
    10621 caccgaacca aactcttgga taagcgtggt cgctgtttta tcaccaattc cgggcacgcc
    10681 gataatattg tccgatggat cgcctttgag acctttaaag tccgcgacca aagacggtgc
    10741 gaatccataa cgttcccgaa ccgccttttc gtcataggtt accgtgtcgg tcaaaccttt
    10801 acgcaaagtg taaactacca cttcgttgcc tcggaccaat tgcagagtat ccatatcgcc
    10861 cgaggcaata atgatttgca aatttttttc atccttcaat ttttctacga ttgtgccgat
    10921 aacatcatcc gcctcaaaac ctgccgcgtc ataaatcgga ataccaaaag cggcaaaaat
    10981 atcgcgcgct cgaataagtt gagcaatcaa ttcttcttcg gctttcggcc gtccggcttt
    11041 gtaaccctcg taaacttgct tgcgaaaagt ggcctccgcc cggtcaaaac aagcggctaa
    11101 ataatcaggc ttaaaatcgg caataatgcg catcagcatt gttgatacac cataaaggcc
    11161 acccgtcggc tcaccctggc tggaggaaaa gtcgggcaag gcgtggtagg cgcgatgaat
    11221 aatcgcatgc gcgtcaaaca aaacaagttt tttggtcttt tccgtcactg cttttagtat
    11281 aacgtaaaaa agaaaaataa gagaaac
//
LOCUS       DAOOLO010000021         9952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_7808_length_9952_cov_5.465596, whole genome shotgun sequence.
ACCESSION   DAOOLO010000021 DAOOLO010000000
VERSION     DAOOLO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7808_length_9952_cov_5.465596"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..575
                     /locus_tag="PLH96_01990"
     CDS             123..575
                     /locus_tag="PLH96_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPB60571.1"
                     /translation="MLHQQIKDEVKKAMLARETERLSAARNVLAAFTNELVAKKQKPD
                     EMLSDDDALAVLKRLAKQRKDSIEQFTIGNRPEMAAAETKELAFIESFLPAQMTREEI
                     QKIAEQKKTELGINDKSKIGQLMGSVMKETKGQADGAVVKEVLEKLLG"
     gene            complement(694..927)
                     /locus_tag="PLH96_01995"
     CDS             complement(694..927)
                     /locus_tag="PLH96_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60572.1"
                     /translation="MSIFTWANRLIKDFDWKDIAATKLSVAFFILAIAKLWPGILGLE
                     WYWYGILFVAFAIKPFAKMFKQGKNPTPAQIQG"
     gene            complement(1020..1571)
                     /locus_tag="PLH96_02000"
     CDS             complement(1020..1571)
                     /locus_tag="PLH96_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPB60573.1"
                     /translation="MILNKKFFQRPTKTVAEDLLGKYLVRRFADGRTLAEMITEVEIY
                     DGPEDRASHACQVGTDKRRRNNKGKTKRTAPMFEAGGIFYIYLCYGMYQMLNIVVGEK
                     DYPAAILIRATRETKGPGRLTRKFAIDKSLNTKSALRGTGLWFEDRKEELAPQKIVRA
                     PRTGVDYAGEIWKNKKYKFFLKT"
     assembly_gap    1745..1844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1845..2386)
                     /locus_tag="PLH96_02005"
     CDS             complement(<1845..2386)
                     /locus_tag="PLH96_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60574.1"
                     /translation="MIDSGVLKTPRLIRAFYALDRKDFVTPDNRSRAYLNQPLPTLRG
                     QTISQPWTVAFILELLSPRPGDRILDIGAGSGWQAALLGFMVSHDDEGNELPAGESGY
                     VVSLEVVPEICDFGRQNLAKYNYLKKGIVEYHCLNAKHGFVDKAPYDHIVAAAALPPA
                     PTGGEESSDVPLEWFSQLKVG"
     gene            complement(2591..3496)
                     /locus_tag="PLH96_02010"
     CDS             complement(2591..3496)
                     /locus_tag="PLH96_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HPB60575.1"
                     /translation="MTKVTMIGAGEIGRAIGKILASAGHKVTYWDKNESLIFDMGEKC
                     LSLPEALAEAETVFFCVPSWSLGEALAFTAPYFKKGTVAVSVTKGIGENNKLLVDEAL
                     KKALPTGAPTVVLSGAMIAEELLAGHGGMAVAASPNKKAAGQVADLFADTSIKVTIVA
                     DARGVAAAGVLKNIYALSLGIADGLGWGRNEQGFLMAQALQEMSKLMVWLGGKKATLF
                     EPAILADFFATSTSKDSANKQAGIELAKHGSALIKSESVASLPALVKIVGRDKVKKLP
                     ILFTLGRVVLGKIEAEKAFEDLKKL"
     gene            3622..4008
                     /locus_tag="PLH96_02015"
     CDS             3622..4008
                     /locus_tag="PLH96_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60576.1"
                     /translation="MSRKRECPACGQKKFLTRHHVFPKRHWPEIANTNRWSGHPPPPI
                     ISICRKCHDKLEKLIPSEIQPFKFYWETTSFFLGLEDVGNVYNLAGLPFWGEPELESS
                     TTSDDGYLTAIQQPQEKVSYARLNKI"
     gene            complement(4124..4528)
                     /locus_tag="PLH96_02020"
     CDS             complement(4124..4528)
                     /locus_tag="PLH96_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60577.1"
                     /translation="MDPLTILLAKVLGVYLVVIGIALFARRKYFLAVFAGFVENRLLR
                     MVVAIAELVAGLFLVLTHQIWSSWPAGIITFFGWAMAVEGVAYLFVPDRWINKMFRLF
                     NTKVWYFVGGILAVVIGLYLISVGFGITLTQI"
     gene            4721..6382
                     /locus_tag="PLH96_02025"
     CDS             4721..6382
                     /locus_tag="PLH96_02025"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPB60578.1"
                     /translation="MKHQHKYIFVIGGVMSGVGKGVTASSIAKILKARGFKVNPLKID
                     PYLNVDAGTMNPIEHGEVFVLDSGLETDQDMGNYERFLDQSLPPENYMTSGMVYKYVI
                     DKERALGYGGRCVDPVPYICEEIHRRIEVSRRKTNSDISIIEIGGTVGEFQNEMFIEA
                     ARILKLKNPNSVLFVLVSYLPTPNKIGEMKTKPTQYAVRSLNSYGVHPDIIIARSDRP
                     LDQKRKEKIAFASNIPADHIISAPDIESIYDIPLNFEKDGLSETLLSLLKLKPKTTDL
                     KDWRQMVAKGARAKDIIKIGIVGKYFRTGDFVLSDVYISVIESLKHSATALGKKIKID
                     WLDSGDYELGVASPTEFKEHFKNLANYHGLLVPGGFGERGVEGKIKVIEYARQNKIPY
                     FGLCYGMQLAVIEYARHVLGLSDAHTTEINPDTKHPVIDIMPEQKKLMTEKNYGATMR
                     LGAYPTLLRKGTIACDSYKAGQANERHRHRFEVNPEYIESLEKAGLIFSGKSPDGVLM
                     EIAELPKTKHPFFVGTQFHPEFKSTPLHPHPLFSAFVKAATQRKK"
     gene            complement(6524..8320)
                     /gene="typA"
                     /locus_tag="PLH96_02030"
     CDS             complement(6524..8320)
                     /gene="typA"
                     /locus_tag="PLH96_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004276097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPB60579.1"
                     /translation="MEIRNIAIIAHVDHGKTTLTDAILRQTDMVPDGFSMDTNTLEKE
                     RGITIYSKNASLFYKGTKINIVDTPGHADFGSEVERVLRSIDSVLLVVDAQEGPMPQT
                     RFVLKKSLELGLKPIVILNKIDKPAADPRRVEEEVLELFMDLGAKDDQLDFPIVYAIG
                     RDGVAKKDLDDEAKGIFPILDLILEKVPAAENNSQAPLRAQPFNLAYDNYLGRMAICR
                     IYEGSIKSGAAVFVKNTTGETYSGKISKLFTFRGLEREEVDEAVAGDIAIIAGIPKIF
                     IGETICTKPESEPLPAIKVDEPTIALDFMINDSPFVGRDGKFVTSRQIRERLEKELEV
                     NVGLKIDFSTEKFKVFGRGELHISILLENMRREGFELAVSQPRVIIKEIDGVKNEPFE
                     EITIDTPAEFQGAIIEKLGQRGAVMTHLKQAGNVSRLMFEGPTRGLLGYRNQFIIDTR
                     GEGIMSSRVIGFRKCAGEIKKTSFGSMVSMITGKAVAYALDNLETRGTLYIGPGTEVY
                     EGMVIGNTAKGEDIVANPIKGKQLTNMRAAGSDENIMLAPPFILSIERGLEVMSEDEY
                     LEITPGSVRLRKAFLTENERSRAKRTTFKPGG"
     gene            complement(8517..8801)
                     /locus_tag="PLH96_02035"
     CDS             complement(8517..8801)
                     /locus_tag="PLH96_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60580.1"
                     /translation="MEVDSELQKVIDDYVEQIQEKEEDVLRLTEGIERVTKEAQKRLA
                     EIEAKLNEQFEKEEITEEEYLLRFREGKEKAIQEAKDEFNALIFVSPDVQ"
     gene            complement(8808..9164)
                     /locus_tag="PLH96_02040"
     CDS             complement(8808..9164)
                     /locus_tag="PLH96_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60581.1"
                     /translation="MTTLENILKKREARLRSPAFQAEINKEKAEFQGILRNLGNNALE
                     AGLTFFFRGPLKMTANTLKLMYAKNYDAQRYGKDAFKIFFGKNGVMHSTVRVTANALH
                     LTSKSVKIGFRKLIAK"
     gene            complement(9295..9885)
                     /locus_tag="PLH96_02045"
     CDS             complement(9295..9885)
                     /locus_tag="PLH96_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60582.1"
                     /translation="MKKFIYILIMVALVVGVYYLYWTLENDSVGQDAGAQLTYSQSDV
                     GLKFDYPGGPEGYVVNERMPVDLGTGLIKNIILMLTEDTLHEPPVGGEGTPVILVSVF
                     ENTKKQFPRAWADENIQYSNINLIMGEVEETVVGGANAIRYMADGLYASENVVVAHGD
                     HVYVITGQFMDQDSPIRHDYQTLVESIRFIPTPEQN"
BASE COUNT         2790 a         2487 c         1870 g         2705 t
ORIGIN      
        1 tcatcatata cagggccggc tttttttgtg ctaagatgat aacaatgtaa aatgtaatcc
       61 gccagctggc ggattaaagt gtaaaatttt taaaaacaaa attaaaagta atgccaaaaa
      121 ctatgttaca tcaacaaatc aaagacgaag tcaaaaaagc gatgcttgcg cgggaaaccg
      181 agcgcctgtc agccgctcgt aatgtcttgg ccgcctttac caacgaactg gtcgccaaaa
      241 aacaaaaacc ggatgaaatg ttgtcagacg atgacgcgct agctgttttg aagcgcctcg
      301 ccaaacaacg caaagattcc attgagcaat ttaccatcgg caatcgcccg gagatggctg
      361 ccgccgaaac caaagagctt gcttttatag aaagtttcct gccggcccaa atgacgcgcg
      421 aagaaattca aaaaattgcc gagcaaaaga aaactgagct gggcataaat gataaaagca
      481 aaattggcca acttatgggc tcggttatga aagagaccaa aggccaagcc gatggcgcgg
      541 tggtcaagga agtgctggag aagctcctgg gataatagcc taacggttag gacacaaaaa
      601 caaaataaaa aatatcgggc gaaagctttg ctctcgcccg atatttttta ttctttcttc
      661 tttctcctca tttctaaccg cccaaaaact atctcacccc tgaatttgcg ccggggttgg
      721 atttttgcct tgcttaaaca ttttcgcgaa cggcttaatc gcaaacgcga caaacaaaat
      781 tccgtaccaa taccactcaa ggcctaagat gccgggccac agtttggcaa ttgccaaaat
      841 aaaaaaggcc acgcttaatt tagtggcggc aatatccttc caatcaaaat ctttaatcaa
      901 ccggttggcc caagtaaaaa tagacatttg tttataaaaa ttagttccta ataaaatttt
      961 taacgaccat ctatattatc tcattatttc ttaatatttt ccattctgtc tttcacattt
     1021 tatgttttca aaaagaattt gtatttttta tttttccaaa tctcgccggc gtaatccact
     1081 ccggtccgtg gcgctcgaac aattttttgg ggcgccaact cttcttttct gtcttcaaac
     1141 cacaggccag tcccgcgcaa agccgactta gtgtttaagc ttttatctat cgcgaattta
     1201 cgcgtcagtc gtcccggacc tttcgtttct cttgttgccc taattaaaat tgccgccgga
     1261 taatccttct cgcctaccac aatattaagc atctgataca tcccgtagca aagatagata
     1321 taaaaaatac cgccggcttc aaacatcggc gctgttcttt ttgtttttcc tttgttgttt
     1381 cgtctccgct tgtcagttcc aacctgacaa gcatggctag ccctgtcttc cggaccatcg
     1441 taaatttcca cttccgtaat catctccgcc aaagtccggc cgtcagcaaa tcgtctaacc
     1501 aaatatttgc caagcaaatc ttccgctact gtcttggtcg gtctttgaaa aaactttttg
     1561 ttcaaaatca ttttattctt aataactttc aggttttgta ttattttatc ggcgcaaagc
     1621 ggcgttttat ctgcgctaga ctttggtctt tacacaagtg gaacaaacac aaacccttca
     1681 aaatcttctt tttcaaaatt actttccccc gtctttctga ttaatctgat tctggaacca
     1741 accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccaact ttcagttgtg
     1861 agaaccactc cagcgggacg tcagaagatt cttcaccgcc agttggcgcg ggaggcaacg
     1921 ccgcggccgc cacaatatgg tcataaggcg ccttgtcgac aaatccatgt ttggcgttta
     1981 gacagtggta ttccactatc ccttttttta aataattata tttggccaag ttttgccggc
     2041 caaaatcaca aatttccggc acaacttcca agctgaccac gtagccgctt tctcccgccg
     2101 gcagctcatt gccctcgtca tcgtggctaa ccataaagcc caacaaagcc gcttgccaac
     2161 cagagccggc gccaatatcc aaaatcctgt ctcccggcct tggtgacaaa agttccaaaa
     2221 taaaagcgac tgtccatggt tgagagatag tctgtccacg caaagtcggc agaggttggt
     2281 tgagataagc gcgactacga ttatccggcg tcacaaaatc tttgcggtcc aacgcgtaga
     2341 aagccctgat taatcgtggc gttttgagca ccccagagtc aatcaattct ccagttaaaa
     2401 ctcgttggtt cactctccca ctctaaaacg aaaaagagat aaagtaaacc tctaataacg
     2461 cggaatgaac gcgcctgccc gccttgggtg aggaataaaa actaataaaa atttgttagc
     2521 ttgcgtcaaa tcttcttctg gtctacattt tttattcgcg gattcgttca attcgcagat
     2581 tcgcattgtg tcaaagcttt ttcaaatctt caaatgcctt ctcagcctct attttgccga
     2641 gcaccacccg acccaaggta aataaaatgg gcaatttttt tactttatcc ctcccaacaa
     2701 ttttgaccaa ggccggaaga gaagccacgc tttcactctt aatcaaagct gacccgtgtt
     2761 tagccaactc tattccggct tgtttgttgg ctgaatcttt actagtgcta gtggcaaaaa
     2821 aatcggccag gattgccggt tcaaacaaag tggccttttt gccgcccagc caaaccatca
     2881 attttgacat ctcttgtaat gcttgggcca tcaaaaaacc ttgttcgtta cgcccccatc
     2941 caaggccatc ggcaatccca agagacaaag cataaatatt ttttaacacg ccggccgcgg
     3001 ctaccccgcg cgcgtctgcg acaatcgtca ccttgataga agtatcagca aaaaggtcgg
     3061 caacttggcc ggcggccttc ttgtttggcg aagcggctac cgccattcca ccgtgaccgg
     3121 caagcaattc ttcggcaatc atcgcgccgg acaaaacgac agtcggcgcg ccggtcggca
     3181 aagctttttt taacgcttca tcaaccaata atttgttatt ttctccaatc cctttggtta
     3241 ccgaaaccgc cactgtcccc tttttaaaat atggcgccgt aaaggccagg gcctcaccca
     3301 gagaccaaga tggcacgcaa aaaaagactg tttccgcttc agccaaggct tcgggcaaag
     3361 acaaacattt ttcgcccata tcaaaaataa gactttcgtt tttatcccaa taagttacct
     3421 tatgcccggc cgaagccaaa attttaccaa tcgcccgacc aatttcgccg gcgccaatca
     3481 ttgttacttt tgtcatactt aagtatttta ccacaaaaca aatttatcct aaattgacta
     3541 attatttagt taagttaagc taaaaaagga gttcttttta ttggcttacg agaccgacgc
     3601 taaaccaaaa agggggagat tatgtcgcgt aaaagagaat gccccgcctg tggtcaaaaa
     3661 aaatttttga ccaggcacca cgtattcccc aaacgacact ggcctgaaat agccaacact
     3721 aacaggtgga gtggtcaccc gccaccgcca atcatctcta tctgtcggaa gtgtcacgac
     3781 aaattggaga agctaatccc aagcgaaatc cagcctttta aattttattg ggaaacaacc
     3841 agttttttct tgggcttgga agacgtcggc aatgtttaca acctggccgg cttgcccttt
     3901 tggggagaac cagagctaga aagctccacg accagtgacg acggttattt gaccgccatt
     3961 cagcagcccc aagagaaagt ctcttacgcc cggttaaaca agatttgacc gggctttggt
     4021 ttatttttct ccctattctg ttcactccca aaacaattga aacaaggccc cgcctcgcgc
     4081 cagtcggcgc gaggcggggc cttttatatt ctttcttcta tgactaaatt tgggtcagag
     4141 tgatgccaaa cccaactgaa atcagatata aaccgataac cactgccaga ataccgccaa
     4201 caaaatacca gaccttggtg ttgaaaagac gaaacatctt gttaatccaa cggtccggca
     4261 caaagaggta agcaacacct tctacggcca ttgcccaccc aaagaaagta ataatcccgg
     4321 ctggccaaga agaccaaatt tggtgtgtca acaccaagaa aagaccggct accaactcgg
     4381 caatcgcgac aaccatgcgc agcaggcgat tttctacaaa gccggcaaaa accgcgagaa
     4441 aatacttgcg acgggcaaac aaagcaatcc caattaccac cagataaacg ccaagcacct
     4501 tggccaaaag tattgtaaga ggatccataa actttttatt aaaaattatt aataactaat
     4561 aaaaaccaac ctattttttc cctaaatttt accatagatg agaaaaactc atcaatcttt
     4621 taaccacgga cttatccact tttgaccaac cttcacccac gaagtccgtc aggtataatg
     4681 gcaagggaaa ataaaattta ttaccaacaa gcgaaataaa atgaaacacc aacataaata
     4741 tattttcgtt attggcggcg ttatgtcagg cgtcggcaaa ggagtaaccg cctcttctat
     4801 cgccaaaata cttaaagccc gcgggttcaa ggtcaatcct ctcaagattg acccttattt
     4861 gaatgtggac gcgggcacga tgaacccaat tgagcacggc gaggttttcg tcctggattc
     4921 cggcttagag accgaccaag acatgggcaa ttacgaacgc tttttagacc aaagtctgcc
     4981 gcctgaaaat tatatgacta gcggcatggt ctataaatat gtgatagaca aagaacgcgc
     5041 cctgggttat ggcggtcgtt gtgttgatcc ggtcccttac atttgcgaag aaattcatcg
     5101 ccggatagaa gtctctcgcc gtaaaaccaa ctccgatatc tctattattg aaatcggcgg
     5161 gacggtcggc gaattccaaa atgaaatgtt cattgaggcg gcgcgtattc ttaagttaaa
     5221 aaatcccaac agcgtcctct ttgtcttggt cagttatctg ccaaccccca acaaaatcgg
     5281 tgagatgaaa accaaaccga ctcaatacgc ggtccgcagt ctaaactctt atggtgttca
     5341 tcctgatatt atcattgccc gctctgatcg ccccctagac caaaaacgca aagaaaaaat
     5401 cgctttcgcg agcaatattc cggccgatca tattatctcc gcacccgaca tagagagtat
     5461 ttatgatatt ccgctcaatt tcgaaaaaga cggtttgtct gaaaccctcc tgtcacttct
     5521 caagttaaag cccaagacaa ccgatctcaa agattggcgt caaatggtcg ccaaaggcgc
     5581 gcgcgccaaa gatatcatca aaatcggcat cgtcggcaaa tatttccgga ccggcgactt
     5641 tgtcctttcc gatgtctata tctcagtcat tgaatcactc aaacattccg ctactgcctt
     5701 aggcaaaaaa atcaaaattg attggcttga ttccggcgac tatgaattag gcgtcgctag
     5761 tccaaccgaa ttcaaagagc actttaagaa tttggcaaac tatcacggcc tccttgtgcc
     5821 aggcggcttt ggcgaacgcg gtgttgaagg gaaaataaaa gtgattgaat acgcgcgcca
     5881 aaataaaatc ccctactttg gtctatgtta cgggatgcaa ttggcggtta ttgaatacgc
     5941 ccgtcatgtt ctcggtttgt cggacgctca cacaacggaa atcaacccgg ataccaaaca
     6001 cccggttatt gatattatgc ctgaacaaaa gaaattaatg accgaaaaaa attacggcgc
     6061 caccatgcgt ttgggcgctt atccgactct tttgcgaaaa ggcactattg cttgcgactc
     6121 ttacaaagct ggccaagcca acgaacggca tcgccaccgt tttgaggtaa atcctgaata
     6181 catagagagc ctggaaaaag ccggtttgat tttttccgga aaatcgccgg atggtgtttt
     6241 gatggaaatc gccgaattgc ccaagaccaa acatcctttc tttgtcggca cccaattcca
     6301 ccccgaattc aaatctactc cgcttcatcc tcacccgctt ttctctgcct tcgtcaaagc
     6361 ggcaactcag agaaaaaaat agagcaagta tcgtcggcaa ttggtatcaa aggaggtccg
     6421 acctccttct taatagggta tcctctctct gaagctgtta cgtagcatgt tacgtaacat
     6481 gctacgtaac agctttttat gctttaattt tacatctctc cttctatccc ccgggtttaa
     6541 aagttgtccg tttggcgcga gaccgctcat tctcggtcaa aaacgccttc cgcagacgaa
     6601 ccgagcccgg cgtaatctcc aagtattcat cttccgacat cacttcaagt ccccgttcaa
     6661 tactcaaaat aaatggtggc gccagcataa tgttttcatc agacccagcc gctcgcatgt
     6721 tagtcaattg tttgccttta attgggttag cgacgatatc ttcacccttc gccgtattgc
     6781 cgataaccat cccctcataa acttccgtgc cgggaccaat atagagcgtc ccgcgtgtct
     6841 ccagattgtc caaagcgtaa gctaccgcct tgccggtaat cattgaaacc atagaaccaa
     6901 aactcgtctt ctttatctcg ccggcacatt ttcggaaacc gataacccgt gaagacatga
     6961 tgccttcacc ccgtgtgtca ataataaatt gattgcggta acccaacaac ccgcgagttg
     7021 gtccttcaaa catcaaacga gaaacattac cagcctgttt tagatgagtc atcaccgcgc
     7081 cccgttgacc tagcttctca ataatcgcgc cttgaaactc cgccggcgtg tcgattgtga
     7141 tttcttcaaa cggctcgttt ttgacgccat caatttcttt gataattacc cgcggttgcg
     7201 aaaccgccaa ctcaaaaccc tcccgacgca tattttcaag taaaattgaa atatgaagct
     7261 cgccccgacc aaatactttg aatttttcag ttgaaaaatc aattttaagc ccaacgttta
     7321 cttcaagctc tttttccaat cgttcgcgaa tttgacggct ggtaacaaac ttcccgtctc
     7381 gtccgacaaa tggggaatcg ttaatcataa aatcaagcgc gatggttggc tcatctacct
     7441 taatcgccgg caagggttcg ctctctggtt tagtacaaat tgtctcacca ataaaaattt
     7501 tggggatacc ggcaattatc gcgatgtcgc cggccaccgc ttcgtctacc tcttcgcgct
     7561 ctaggccacg aaaagtgaag agttttgata ttttgcctga ataagtctct ccggtcgtat
     7621 tcttaacaaa aaccgccgcc cccgatttga tggaaccttc gtaaattctg caaattgcca
     7681 tccgtccaag atagttatcg taagcgagat taaaaggctg agcgcgtaaa ggcgcttggg
     7741 agttgttttc tgcggccggc actttttcca aaattaaatc aagaattggg aaaatacctt
     7801 ttgcctcgtc atccaaatct tttttagcga ccccatcacg accgatagca taaacaattg
     7861 gaaaatccaa ctgatcatcc ttggctccca agtccataaa caattctagg acttcttctt
     7921 ctactctccg cgggtcagcc gctggcttat caattttatt caagataaca atcggtttga
     7981 gccccaattc caaagatttt tttaagacaa agcgagtttg cggcattggc ccttcttgcg
     8041 cgtcaaccac caaaagcacc gaatcaattg agcgcaagac tcgttccacc tctgaaccaa
     8101 aatcagcgtg gcccggcgta tcaacaatat tgatttttgt tcccttataa aaaagggagg
     8161 catttttgga ataaatagta attcctcgct ccttttccaa ggtattggta tccatactaa
     8221 aaccgtccgg caccatgtca gtctggcgaa gaatagcatc ggtcaaagtt gtcttcccgt
     8281 ggtcaacgtg ggcaataatc gcgatatttc tgatttccat ttttttattt cacagagttt
     8341 ttaacaaaaa agtctccctc gggggagaac acggtttgat ctctgctctt acttatattc
     8401 taaaacaaat ctttcaagat agcaagaaaa gtgagcaacg aaaactgttt gcgtcccccc
     8461 tgcttatcac gttagctgtc tttggtcgtt ttctaaataa cacaaggttt ttggttttat
     8521 tgcacgtcag gacttacaaa aattaaagca ttaaactcat ctttggcctc ctgaatagct
     8581 ttttcttttc cttccctaaa acgcaaaaga tattcttctt ctgttatctc ttctttttca
     8641 aactgttcat tgagctttgc ctcaatttca gccaaacgtt tttgcgcttc tttagtgact
     8701 cgttcaatcc cttcggtcaa tcgcaaaaca tcctcctcct tctcttgaat ttgttcaaca
     8761 taatcatcta tgactttttg caactcagag tccacttcca taaaatttta ttttgcgatt
     8821 aattttctaa aaccaatttt taccgactta gatgtgaggt gaagagcgtt tgcggtcact
     8881 ctaaccgtgc tatgcattac cccattttta ccaaagaaaa ttttaaaggc atcctttccg
     8941 taccgttggg catcataatt tttagcatac attaatttaa gtgtgttcgc cgtcatcttt
     9001 aacggtcctc taaaaaagaa agtcaaaccg gcctctaagg cattgtttcc caaatttctt
     9061 aaaattccct gaaactcagc tttttcttta tttatttctg cctgaaaagc aggcgaacgg
     9121 agcctggctt cccttttttt taaaatattc tccagagtcg tcattccttt gattatataa
     9181 tactacaccc aaacaaacaa tttaagcgtt tcaacccaat ttcccagaaa ttaacacttg
     9241 gataaactga gtactaacca gcctcctcct tttatggcta atatcgttaa tattttaatt
     9301 ctgttcgggc gtggggatga agcggattga ctcaactaaa gtttgatagt catgacgaat
     9361 cggagagtct tgatccataa attgaccggt gatgacgtat acgtgatccc catgagcaac
     9421 aaccacgttt tcagacgcat acaaaccgtc agccatatag cggatagcgt tagctccgcc
     9481 taccaccgtt tcctcgactt cgcccataat gagattaata ttgctatact gaatattctc
     9541 atctgcccaa gctctcggga attgtttctt agtgttctca aagaccgaga caagaataac
     9601 cggcgtgcct tcgccgccga ccggtggctc gtgaagggta tcctctgtca gcatgaggat
     9661 gatatttttt atgagcccgg tacccaggtc tactggcatt ctctcgttaa ccacataacc
     9721 ctccggccct ccaggataat caaacttgag accgacgtca ctttgactat aggtaagttg
     9781 cgccccagca tcttgcccga cgctatcatt ttccaaagtc caatagagat agtaaacccc
     9841 tacaacaaga gcgaccatga taagaatgta tataaacttt ttcatatttt tataaaatat
     9901 tttgctcttt attcataatt ttagggcttg cactgagctt gccgaagtgc tg
//
LOCUS       DAOOLO010000022         9754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_8068_length_9754_cov_7.195072, whole genome shotgun sequence.
ACCESSION   DAOOLO010000022 DAOOLO010000000
VERSION     DAOOLO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8068_length_9754_cov_7.195072"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..108
                     /locus_tag="PLH96_02050"
                     /pseudo
     CDS             1..108
                     /locus_tag="PLH96_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            complement(366..2732)
                     /locus_tag="PLH96_02055"
     CDS             complement(366..2732)
                     /locus_tag="PLH96_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPB60583.1"
                     /translation="MNATSLILTILVSAAALAIAIVLFFWWRRRHLANLDLKILAIRI
                     PRHDKEDGERDFAKQINLSEQLFVALSSIGRPFVFEASVLNTGEEIRFYLAVPRASLE
                     FARKTVQGFFLDAQVEETSDYTIFETGSQAAAGYLSLDKHFVLPVASYRESEADIFSP
                     ILSTLSKLAENDEGASIQVVVSPTGDTAKKEIAHILEKLREGKSLKELTDTGLSLKKI
                     GEEIGKAVFDKKYEKDEGPKLADEEMVKAVQLKIAKTLFAVNVRIVTAASTKGRAEDI
                     LMSVSSSFSKLASPVRNSFKVVKPKKINQLVFRYVFRETDDRRAMILNAEELAGIFHL
                     PTETSDVPNVTWLRAKEAPPPEVLPPTGLVLGQSIFRGVEKTIRVTDEDRRRHTYIIG
                     QTGTGKSVFIQNMAMQDMKEGKGFCVIDPHGELVDKILNFVPASRIDDVIIFDPGDRS
                     RPLGLNMLEYNLSRPEEKTFIVNELQAIFNQLFMKETMGPQFEKYMRNTLLLLMDDFK
                     NEPATLMEVPRVFTDEEYRRRKLANCTTPLVVDFWTKEVPKTSGEQSLGNFAPYITSK
                     FDGFISNDYLRPIIGQTKSSFNFRQAMDEGKIILVNLSKGKLGDLNSALLGMLIVGKL
                     LQAALSRVDVADEKVRRDFYLYIDEFQNYTTDSIATILSEARKYRLNLTIAHQFIAQL
                     KDNIRESVFGNVGNLVAMRVGPPDAEVLVKHFGPVFSERDLISIENLNAYVKILVDGQ
                     PSEPFNIKFKWAEGGSSEVRKQMKELSRLRYGHDLQTVEEDIVRRLRD"
     gene            complement(2734..3174)
                     /locus_tag="PLH96_02060"
     CDS             complement(2734..3174)
                     /locus_tag="PLH96_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60584.1"
                     /translation="MAENFLGNSQAIEAEIAQLQKMIEVKRNQLEAQSGIVEEKELVR
                     SAIQEMITESAETDSKAALSQPVVAAKTKVVPTGNASGASYLDRLDDESAVKLNAYIA
                     EIGQHGIVRTLNKVVAEEPFIIDAFHDVLVDKLYEELKTKGLIK"
     gene            3239..>5112
                     /locus_tag="PLH96_02065"
     CDS             3239..>5112
                     /locus_tag="PLH96_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB60585.1"
                     /translation="MFYLDELNDPQREAAKHKDGPLLVIAGAGAGKTRTVACRVLHLI
                     KSGVVPNQILAITFTNKAAREMKERILSLLAKDSKAEGGLKQGQNRAEPFVSTFHALC
                     VHILRNEHHALGLPKFFSIFDRDDSISKIKSAMKRVDVDPKRFEPGKILSTISRQKGN
                     GVGLEEYRASARDYYPKTVSAVWAEYAKLLKKENALDFDDLLVQTVTLFKQRPEILAK
                     YQDRFRYISVDEYQDTNVIQYELVKLFAEKYKNVCVVGDMDQSIYGWRGADYTNLLRF
                     EKDYPNAKTVLLEENYRSTETILTAANRIIAKNTRRFDKKLFTKKKGGAKIGLYAGME
                     EGDEARFIARRAQEMIAEGTKPEQLAVLYRANFQSRVLEEAFLRAGVPYQVLGTRFFE
                     RKEIKDIVSYIKAALNPTDFEALKRVINTPPRGLGQVTLTKLAAGAEETLPATTREKI
                     AKFRRLLSEIANFAQSHKPSELIKFVLEKTGLEEGLKKGGEDSLERLENAREFASLAV
                     RYDHLPPAEAVPIMLAEIALSSEQDSLNENKNGAKLMTVHAAKGLEFDVVFVTGLEEG
                     LFPHRGFDETDRDEEEERRLFYVAVTRARQKLYLTYAQSRLVFGSRQVNLPSEFIFDI
                     "
     assembly_gap    5113..5212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5323..6123
                     /gene="rsmA"
                     /locus_tag="PLH96_02070"
     CDS             5323..6123
                     /gene="rsmA"
                     /locus_tag="PLH96_02070"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPB60586.1"
                     /translation="MKAKKSLGQNWLKSETAIQEIIKTADLNERELVLEIGPGQGKLT
                     EALLSVGATVVAIEKDDRLIEVLTEKFAAEIKVGRFILIHDDILNLDFSSLFSSLKTN
                     RRKLIASYCLVANIPYYLTGQIIRKFLSQEKIQPTKMVLMLQKEVAKRIVAGDGKESL
                     LSLSVKAYGEPKYIKTVPAGAFVPKPKVDSAILLIHNISRNNFKAGEETRFFDLLRQG
                     FSQKRKKLGNRLDLSDQAWQVCHLDPNARAEDLRLEDWLCLIDADSRR"
     gene            6363..6854
                     /locus_tag="PLH96_02075"
     CDS             6363..6854
                     /locus_tag="PLH96_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPB60587.1"
                     /translation="MFNFKIYTFISLGLIAFWYIMADGRAPIFWPSFSLVEAKTETGL
                     LEPNTLVINEQKIKVDIAATPEKRTRGFAGYEYLPDGTGFLYVFDEPDFYSFWLKDVT
                     FPIDIVWISYYHDVVDIAAYLTPQTSPKILEPKKPAKYILILPAGATEKLRLEIGDEV
                     LGL"
     gene            complement(6855..7916)
                     /locus_tag="PLH96_02080"
     CDS             complement(6855..7916)
                     /locus_tag="PLH96_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HPB60588.1"
                     /translation="MKKFKTTTPFLFLFLAVLVGFGSFGFGANKAHAQIGQVPFGGWI
                     TKIEPCIISNNVMIRHAPAAGPGVVMYDPKISHLYAYAQLMPGNWMLGNTLGVEPCLQ
                     LAPFPPFVVLRGFFPLITIVGTSGTGMTAGSGSNTSGSNTDNADNPDNAGGSSRGDVD
                     GVVPAIYEPVEPCGEGDNTASSYVIANEGWRNSPYTDSLGHRTVGVGHRITGDEPFNV
                     NQTLTNQQVATLFEQDYNRAKEEAAMAAALHGVDFDSLSDTRQAVLTDMAFNMGAQGG
                     GGLAGFNNMWGEIAQGDWDGAAGEIQNSTYARQLPGRSELNAEAMRTNDRTVLDRHIN
                     SDPQAWQFCQPVSEPPIIA"
     gene            complement(7916..8767)
                     /locus_tag="PLH96_02085"
     CDS             complement(7916..8767)
                     /locus_tag="PLH96_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPB60589.1"
                     /translation="MFNFYKLRNCLKLKRRVFLFFPLVLLLWWPLVSAGFVFEKIVTE
                     GEEDPSIKNLQIILNQDSRTQVAKVGAGAPGAETSFFGARTRGALARFQRLNGISGEY
                     GFVGRKTRLALNKKLAESYFAETAASEDKLTDQLAKTQKTFVFRLPSDIRGNVTRLAD
                     KKSPYIKRLEETEKSESKKSQVVAGKPKITSLSPARGAYGVMVKIKGENFTATGNTIY
                     TGYSVLKNVSSPDKETLTFTVQAFGAEYQQSRSLTNHFESFSIPLLVYVENANGQSNS
                     LTFNYQF"
     gene            8867..9568
                     /locus_tag="PLH96_02090"
     CDS             8867..9568
                     /locus_tag="PLH96_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60590.1"
                     /translation="MNRLPSRKVIFFIVILLIITLGVLWYVGSKNRAERERREAEAIA
                     EIINKNESLRNIVEDNLEKKGGLQSFDPASLFENNDIQLDAMANVTAADLQTYAERLV
                     GALKPISGNRKHEVRIMLEAMDSGDRTMIQDVVSARLTFDSIADNLAKIPAPAEMATF
                     QKDLIISLQKLSALAAQMEKALDEPILALQSGNVYLAETANLINLADKLNNYLQQTGV
                     VGPEYKIVITFEKDN"
     gene            9568..>9754
                     /locus_tag="PLH96_02095"
     CDS             9568..>9754
                     /locus_tag="PLH96_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60591.1"
                     /translation="MKPEAQKITLFIASALLTFGLAYSAQAITSGADLFGDFLPNSDG
                     LPDIVNLADYNLSDFGDF"
BASE COUNT         2679 a         2312 c         2101 g         2562 t
ORIGIN      
        1 agcagtttag cggtttattc cggacaaaac gtagaaaaag gagacattgt tggctatgtc
       61 ggttccaccg gccgttctac cggcaaccat ctccactttg aagttcgtgg cgctgccaat
      121 ccattctaat gcggataaag cgcagattta cgcggaaaga aagagggttt taggttttag
      181 acaaggtaag aatttacagc cgacagtcag aagaataaat caaagcgtgg caccatcaga
      241 tgccgcgctt tgatttattc ttgggccttt cttttaattt ttaattctaa tccggctgtt
      301 agttgtaggc tgtaaactag ttccttgtct gcgtttcatc tgcgtaaatc tgcgctttat
      361 ccgcattagt cccggagtct tctcacaata tcttcttcta cggtctgcaa atcgtggcca
      421 taccgaagtc gtgacagttc tttcatctgt ttgcgcactt cactagaacc gccctccgcc
      481 catttaaatt tgatgttaaa aggttcactt ggttgaccgt ctaccagaat tttgacataa
      541 gcgtttagat tttcaatact aatcaaatct cgttcactaa acaccggacc aaaatgttta
      601 accaaaactt cggcgtcagg cgggccaacg cgcatcgcta ccaagttgcc gacgttacca
      661 aagaccgatt cccgaatatt atctttaagc tgagcaatga actggtgtgc aatcgtcaaa
      721 ttcagccggt atttgcgcgc ctccgacaaa attgtggcaa tagagtcagt tgtgtaattt
      781 tgaaactcgt caatataaag ataaaaatca cggcggactt tttcatcagc cacatcaact
      841 cgcgacaagg ccgcttgtaa aagcttgcca acaatcaaca tgcccaaaag cgccgagttg
      901 aggtccccca atttaccctt ggataaattg accaggataa ttttgccctc gtccatcgcc
      961 tggcgaaaat tgaatgacga cttggtttgc ccgataatcg gtcgaagata gtcgttggaa
     1021 ataaagccgt caaacttgga ggtaatataa ggcgcaaagt tgcccaaact ttgttcaccg
     1081 cttgtcttgg gtacttcctt ggtccagaag tcaaccacca agggagtggt gcagttggcc
     1141 aatttgcgac gacggtattc ttcgtcggta aagacgcgag gcacttccat caaggtcgcc
     1201 ggctcatttt tgaaatcatc catcaaaagc aacagggtgt tcctcatata cttttcaaac
     1261 tgcggaccca ttgtctcttt cataaagagt tggttgaaaa tcgcctgcaa ttcattgaca
     1321 ataaatgttt tttcttccgg acgggagagg ttgtattcaa gcatattaag acccaagggt
     1381 cgactgcgat cgcccgggtc aaaaataatc acgtcatcaa tccgactagc cggcacaaaa
     1441 tttaaaattt tgtccaccag ttcgccgtgg gggtcaatca cgcaaaaacc tttgccctct
     1501 ttcatatcct gcatcgccat gttctgaata aagaccgatt tgccggtacc agtttgacca
     1561 atgatataag tatggcgacg gcggtcttcg tcggtcacgc gaatggtttt ttccactccg
     1621 cggaaaatac tttgccccaa aaccaagcca gtcggaggca aaacttcggg cggcggcgcc
     1681 tctttggccc gtagccaagt gacgtttggc acatccgaag tttcagtcgg caagtggaaa
     1741 atgcccgcca actcttcggc attcagaatc atcgcccgac ggtcatcagt ctcgcgaaaa
     1801 acataacgaa aaaccaattg attaattttc ttgggcttga ccaccttaaa tgaattacgg
     1861 accggcgaag ccaatttgct aaacgaggag gagacagaca tcaagatgtc ttctgcccgc
     1921 cccttagtgg aagctgccgt aacgatccgc acattgacag caaacaaagt tttggcaatc
     1981 ttcaactgaa ccgccttaac catttcctcg tcagccaact tgggaccttc atctttttcg
     2041 tattttttat caaacaccgc cttaccgatc tcttcaccta ttttttttaa tgacaagccc
     2101 gtgtctgtca attcttttaa agacttgcct tcgcgtaatt tttccaagat atgggcaatt
     2161 tcttttttgg ctgtgtcgcc ggtcggcgag accacaacct ggattgaggc cccttcgtca
     2221 ttttcggcca gctttgacaa agtagaaaga atcggcgaaa agatgtcagc ttcagattca
     2281 cgataagaag cgaccggcaa aacaaagtgt ttatcaagcg acagataacc ggccgcggcc
     2341 tggctgccgg tttcaaaaat agtataatcg gaggtttcct caacttgcgc gtctaaaaag
     2401 aaaccctgca ccgttttgcg cgcgaactcc aacgaagcgc gtggcacagc caaataaaaa
     2461 cgaatctctt cgccggtgtt cagcactgag gcctcaaaaa caaaggggcg accgatagaa
     2521 gagagggcaa caaaaagttg ttctgacaag ttgatttgtt tggcaaaatc tctttcacca
     2581 tcttctttgt cgtgacgcgg aatcctaatc gctaagattt tcaaatccag attggccaaa
     2641 tgacggcggc gccaccaaaa aaagagcaca attgctatcg ccagggcagc cgcggaaaca
     2701 agaatggtta aaatcagcga tgttgcgttc atattatttt atcaacccct tggtttttaa
     2761 ttcctcatac agcttatcaa ccaaaacgtc gtggaaggcg tctataataa aaggttcttc
     2821 ggccaccact ttgtttaggg tgcggacaat accgtgctga ccaatttcgg caatataagc
     2881 atttaactta acagccgact catcgtccaa acgatcaagg tagctggcgc cggaagcgtt
     2941 gccagttgga acaactttcg tctttgccgc cacgaccggc tggcttaagg ccgctttcga
     3001 atctgtttcc gcgctctcgg ttatcatttc ttgaatcgcg ctccgaacca gttctttttc
     3061 ttccacgatg ccgctttgcg cttcaagttg gttacgctta acttcaatca ttttttgcaa
     3121 ttgcgcaatt tccgcctcaa tcgcttggct gttgcccaaa aaattttctg ccataaaaaa
     3181 ttaattagtg attagagttt atatgtttca attatattat gataatatct actagtctat
     3241 gttttatctt gacgaattaa acgacccaca gcgcgaggcg gccaaacaca aagacggacc
     3301 actcttggtt atcgccggcg ccggcgccgg caaaacacga acagtcgctt gccgcgtttt
     3361 gcatttaatc aagtccggtg tggtgccgaa ccaaattttg gccatcacct ttaccaacaa
     3421 agcggccagg gagatgaagg aacgtatttt gagtttgttg gccaaagaca gcaaggctga
     3481 aggggggttg aaacaagggc aaaatcgcgc cgaacctttt gtctccacct ttcacgcgct
     3541 ttgtgtccat attttacgga atgaacacca cgccctggga ttgccaaaat ttttttctat
     3601 ttttgaccgc gatgattcta tctccaaaat caaaagcgcg atgaaaagag ttgatgttga
     3661 ccccaaaagg tttgagccgg gcaaaatcct ctcaaccatt tctcgccaaa aggggaatgg
     3721 ggttggtctg gaagaatacc gcgccagcgc gagagactat taccccaaaa ccgtttcggc
     3781 tgtttgggcc gaatatgcca aactgcttaa aaaagaaaac gctttggact ttgacgattt
     3841 gttggtgcaa accgtgacgc tctttaaaca aaggcccgag attctcgcca aataccaaga
     3901 ccgcttccgt tatatttcag ttgatgaata ccaagatacc aatgttattc aatatgaact
     3961 ggttaaacta ttcgccgaaa aatataaaaa tgtctgcgtc gtcggcgata tggatcaatc
     4021 tatttacggc tggcgcggcg ccgattacac caacttgtta cgttttgaga aagattatcc
     4081 aaatgccaaa actgtcctct tggaagagaa ttaccgctcc acagaaacca tcctaaccgc
     4141 cgccaaccga attatcgcca aaaacacccg gcgatttgac aaaaaacttt tcaccaagaa
     4201 aaaaggcggg gccaaaattg gtctttacgc cggcatggag gaaggtgatg aagcgcgttt
     4261 tatcgcgcgg cgcgctcaag aaatgattgc cgaaggaacc aagcccgaac agcttgccgt
     4321 tttgtatcgc gctaattttc aatcaagggt tctagaagaa gcttttctgc gcgccggcgt
     4381 gccttatcaa gtcttgggaa cccgattttt tgaacgcaaa gaaatcaaag atattgtttc
     4441 ttacattaag gcagcgctta acccgactga ttttgaagcg ctaaaacgcg tcatcaatac
     4501 accccctcgc ggcttgggac aagttacgct gaccaaactg gcagccggcg ctgaagagac
     4561 cttgccagcc accaccagag aaaaaatcgc caaattccgg cggctactat ctgagattgc
     4621 caactttgct caaagccaca aaccgtccga gctgattaag tttgtgcttg aaaaaaccgg
     4681 cttggaagag ggacttaaaa agggcgggga agacagtttg gagcgcttgg agaacgcgcg
     4741 cgagtttgca agtttggctg tcagatacga ccacctgccg ccagccgagg ccgtgccgat
     4801 aatgttggcg gaaatcgcgt tgtcttccga acaagattcc ttgaatgaaa ataaaaacgg
     4861 cgccaagttg atgaccgtgc acgccgccaa gggattggaa tttgatgtcg tttttgtgac
     4921 tggcctggaa gaaggattgt ttccgcaccg aggttttgat gaaaccgacc gcgacgaaga
     4981 agaggagcga cgtttatttt atgtcgcggt gacgcgcgcc cgccaaaaac tttacctcac
     5041 ttacgcccaa tctcgcctcg ttttcggttc cagacaagtc aatctgccct cggaatttat
     5101 ttttgacatt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattatacc
     5221 atatttgagt tattttttca agcatgttag gattacaaag ttaaactgcc gttctgtttt
     5281 ctgtgtttta ccagcgttaa ccggcatttt atctgcgtta gaatgaaagc gaaaaaatca
     5341 cttggacaaa attggttaaa atcagaaacg gcgatccaag aaataatcaa gaccgctgat
     5401 ttaaacgaac gcgaattggt tttggaaatc ggtccgggac aggggaaatt aactgaagcc
     5461 ttgctgtccg ttggcgcgac cgtggttgct atcgaaaaag acgaccgttt gattgaagtt
     5521 ttaactgaaa aatttgccgc cgaaatcaag gtcggccgtt ttatccttat ccacgatgac
     5581 attttaaacc tagatttctc ttcgcttttt tcttctttga aaactaatcg ccgaaagttg
     5641 attgccagtt actgtctggt cgccaacatt ccatactacc tcactggcca aatcatcaga
     5701 aagtttttaa gccaagaaaa aatccaaccc accaaaatgg ttttgatgct tcaaaaagaa
     5761 gtggccaaac gaatcgtggc aggagatggc aaagaaagtt tattgtcact ttcggtcaaa
     5821 gcttacggcg aaccaaaata catcaaaacc gtaccagctg gcgcttttgt ccccaagcca
     5881 aaagtggact cggccattct cttgattcat aatatctcca gaaataattt taaagctgga
     5941 gaggagacaa ggttctttga tttactgcga caaggttttt cccaaaaacg taagaagttg
     6001 ggtaaccgtc ttgatttatc tgatcaagct tggcaagtct gccaccttga tcccaatgcc
     6061 cgcgccgagg atctaaggtt ggaagattgg ctctgcctaa tagacgcaga ttcacgcaga
     6121 taaaacgcag atttgcgcag acaacaaaac ctagtgacgt cttatccgca ttagatctgt
     6181 tctttatctg cgtttaatct gcggcagtca gcgtcttatc cgcattagat ctgcaccaag
     6241 tctgcatcca attatcttcc ctcccaacca agaatcaaac ccgcaagcaa aacttcccaa
     6301 cttacaaact ggtatttatc tgcgttttat ctgcgtgaat ctgcgtctta tccgcattag
     6361 atatgtttaa ttttaaaatc tacaccttta tttcccttgg cttaatcgct ttttggtaca
     6421 ttatggctga tggacgagcg ccgatttttt ggccgagctt ttcattggtt gaggccaaga
     6481 ccgaaacagg acttcttgag ccaaacacac ttgtcatcaa tgaacaaaaa ataaaagttg
     6541 atattgccgc caccccagaa aagcgaacga ggggatttgc cggttatgaa tatctgcccg
     6601 atggcactgg ctttttgtat gtttttgatg agccagattt ttattccttt tggcttaaag
     6661 acgtcacctt tccgatcgac atcgtctgga tcagttatta tcacgatgtt gtagatattg
     6721 ctgcttatct cacacctcaa acctcaccca aaattcttga accaaaaaaa ccggccaagt
     6781 atattcttat tttgccggcc ggcgccacgg aaaagttgcg tttggaaatc ggggatgaag
     6841 ttcttggatt ataatcaagc gattatcggc ggctcactga ctggctgaca aaactgccaa
     6901 gcttgtgggt cggagtttat atgacggtcc aaaacagttc ggtcatttgt gcgcatcgct
     6961 tcggcgttga gttcggaacg gccaggcaat tgtctcgcgt aagtgctgtt ttgaatttct
     7021 ccagccgcgc catcccaatc accttgggct atttcgcccc acatattatt aaatccggcc
     7081 aagcctccgc cgccttgcgc ccccatatta aacgccatgt ccgtcaaaac agcctgcctg
     7141 gtgtctgaca aagaatcaaa atcaactccg tgaagagcgg cggccatcgc ggcctcttct
     7201 tttgctctgt tatagtcttg ctcaaacaag gtagccactt gctggttggt caaggtttga
     7261 ttgacattaa agggttcgtc gccggtgata cggtggccta ctccgaccgt gcgatgacca
     7321 agcgaatcgg tataggggga gttccgccag ccctcgttgg caataacata agacgaagca
     7381 gtattatcac cttcgccgca aggttctacc ggctcataaa ttgccggtac cacaccgtcc
     7441 acatcgccac ggcttgagcc gccggcattg tcaggattgt cagcgttatc agtattactc
     7501 ccgctggtgt tgctgcccga accagctgtc atccccgtgc ccgaagtgcc aacaatggta
     7561 attaaaggga aaaaaccacg cagaacaaca aaaggtggaa aaggggcaag ttgcaaacaa
     7621 ggctccaccc ccagggtgtt gccaagcatc caattaccag gcatcaattg ggcataagcg
     7681 tatagatggc tgattttcgg atcatacatc accacgcccg ggccagcagc cggcgcgtgc
     7741 ctgatcataa cattgttgct gattatgcaa ggctcaattt tggtgatcca acccccaaag
     7801 gggacctgcc caatttgggc atgcgccttg tttgccccaa aaccaaaaga cccgaatcca
     7861 accaagactg ccaagaaaag aaagaggaaa ggagttgttg ttttaaattt tttcatcaaa
     7921 actggtagtt aaaagtcaaa ctgttacttt gaccgttggc attttctaca taaaccaaaa
     7981 gcggaatcga aaatgattca aaatgattag tcaaactgcg cgattgttga tactcggctc
     8041 caaaggcttg caccgtaaag gtcaaagttt ctttatcagg cgaagaaacg tttttcaaaa
     8101 cactataacc ggtataaatg gtgttgccgg tggcggtaaa gttttcgcct ttaatcttta
     8161 ccatcacgcc ataagcgccc ctggcagggg acaaagaggt gattttgggc tttccggcaa
     8221 caacttgact ctttttgctt tccgactttt ctgtttcttc caaacgcttg atatacggcg
     8281 atttcttatc ggccaaacgc gtaacattcc ctctaatgtc tgaaggaagc ctaaaaacaa
     8341 aggttttttg ggtttttgcc aattggtcag taagtttatc ttcagaggct gccgtttcag
     8401 caaaataaga ttcggccagc tttttgttca aagctagcct ggttttgcgc ccaacaaaac
     8461 catattcgcc ggaaatgccg tttagacgtt ggaaccgcgc caaggcgccg cgggtccgag
     8521 cgccaaaaaa actggtctcc gcgcccggcg cgccggctcc aaccttggcc acttgggtgc
     8581 ggctatcctg attaagaata atctgaagat tttttatgct tgggtcctct tcgccctcgg
     8641 tcacaatttt ttcaaagaca aaccctgccg aaacaagcgg ccaccaaagc aataaaacca
     8701 aggggaaaaa cagaaaaacg cgtcttttta attttaagca atttctcaat ttgtaaaaat
     8761 taaacatttc tcttttataa taattcaatc tgtctttttt atccactttt ttcaataaaa
     8821 aacatttacc aaatacctaa acaaacggta taataaagtt acaatcttga atcgattacc
     8881 cagtcgtaaa gtaatatttt ttatagttat actcctgatt ataactctag gggttttatg
     8941 gtatgtcggt tcaaaaaatc gcgccgagcg agaaagacga gaagcagagg caatcgctga
     9001 aatcataaac aaaaacgaga gcctgcgaaa tattgttgaa gacaacctag agaagaaagg
     9061 ggggctgcaa agttttgacc cggccagttt gtttgaaaat aacgacatcc aattagacgc
     9121 tatggccaat gtaaccgccg ctgatttgca gacttacgct gagcgcctgg tgggcgccct
     9181 taaaccgatc tccggcaatc gcaaacatga agtgaggata atgcttgagg cgatggatag
     9241 cggcgaccgg acaatgattc aagatgtcgt ttccgccaga ctaacctttg actccatcgc
     9301 cgacaatttg gctaagattc cagctccggc cgaaatggcc actttccaaa aagatttaat
     9361 aatttccctg caaaaacttt ccgccctggc cgcccaaatg gaaaaagctt tagacgaacc
     9421 aattctcgcc ctgcaaagcg gcaacgtcta tctggcagaa accgccaacc taatcaattt
     9481 ggccgacaaa ctgaacaatt atctacaaca aacgggtgtg gttgggccgg aatacaaaat
     9541 tgttatcact tttgaaaaag ataattaatg aaaccagaag cgcaaaaaat aacgctgttt
     9601 attgcttcgg ccctgctgac ttttggcctt gcttattcgg cacaggcaat tacatctggg
     9661 gcggacctgt ttggtgactt tttgccaaat agcgatggtt tacctgatat cgttaattta
     9721 gctgattata atttatctga ttttggagac tttg
//
LOCUS       DAOOLO010000023         9684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_8161_length_9684_cov_6.258386, whole genome shotgun sequence.
ACCESSION   DAOOLO010000023 DAOOLO010000000
VERSION     DAOOLO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8161_length_9684_cov_6.258386"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1098
                     /gene="dnaX"
                     /locus_tag="PLH96_02100"
     CDS             55..1098
                     /gene="dnaX"
                     /locus_tag="PLH96_02100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPB60592.1"
                     /translation="MSEQTLYRKYRPQKFSEVLGQEQVTQVLETSLKNGGLVHAYLFS
                     GPRGTGKTSVARLLAKAINCSERDLFEIDGASNRGIDEIRALREAVSTLPFESPYKVY
                     IIDEVHMLTKDAFNALLKTLEEPPAHVVFILATTEPHKVIETIVSRCQTHQFKKPTLE
                     ILQKMVVDVAQKEKVSLAEEVASLVAFLGDGSFRDTLGILQKVISYSPDKKISVEEAR
                     QITGAPSFELVSDFVLALAQSDLESALASLDKVAEQSLEMKVFLKIAMREIRFLILSV
                     FAPRLKTKVAGELTVASRTFLNKPKEEVVTGAKQLASVLREFLDIYSELNDSFIPQAP
                     IELALIKTLSQKP"
     gene            complement(1261..1467)
                     /locus_tag="PLH96_02105"
     CDS             complement(1261..1467)
                     /locus_tag="PLH96_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB60593.1"
                     /translation="MSDAAKRLGENIRRIRLENGMTQGDLCRLLKLDRAYMSNVESGK
                     KNPTLATIERIAVALEVTSDKLLK"
     gene            complement(1695..1765)
                     /locus_tag="PLH96_02110"
     tRNA            complement(1695..1765)
                     /locus_tag="PLH96_02110"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1731..1733),aa:Gln,seq:ttg)
     gene            1954..2868
                     /locus_tag="PLH96_02115"
     CDS             1954..2868
                     /locus_tag="PLH96_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HPB60594.1"
                     /translation="MKKLTYFLIVVCLAAAGWLVYSYYFSNDPVAVTKKFYGGWLAGD
                     YRLSDSSYKNIEAWTDQMKKKAEGLAASFNQGDDDPILCAQDKPDNFLAYEVGSNEEG
                     VRVEVREDFSGTVKTVQVSLIKVGGKWKIDDIVCNDKAAPSNDEEQNLVSDYIKENIS
                     ELSPEKAVLGGTFSVTSLVFGEGQTGVVEYEDGHILLRAGFKYSIGENNVVKIESFEV
                     LPEESGAFSETGNLVKDEGADGWLLVYEKPGQPALRVMLEFGPNSRCQATDGPITCSE
                     WEIGDRAKIDGRRQGNKVAVDLAIEERN"
     gene            2948..3271
                     /locus_tag="PLH96_02120"
     CDS             2948..3271
                     /locus_tag="PLH96_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60595.1"
                     /translation="MTEKSYRLEMILLAFVLILLILLTYGYGMFFLNFYWGQSVVLSP
                     VEAEELAEPPFEEAIYSPAPPGRAQFISPFSPTGGLGAPPDYPKLIRFGDNQPKFVMS
                     GERQV"
     gene            complement(3336..3986)
                     /locus_tag="PLH96_02125"
     CDS             complement(3336..3986)
                     /locus_tag="PLH96_02125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB60596.1"
                     /translation="MGRKYSLLAIIIGLIILVMVIYSLSQSDNLTEPDKNKPMSPTVN
                     TSSIIKLETNLGEIQFETYPADAPETVANFIKLANEGYYDGLIFHRVIDGFMIQGGDP
                     NCSPELSKRPATGPCGTGGPGYQFKDELNPETTSYQAGYQKGVVAMANAGPNTNGSQF
                     FIMLADTPLPHDYTIFGKVIKGQEVVDKIGQVKVGQNDKPLETVRVIKATVIDENS"
     gene            4198..4920
                     /locus_tag="PLH96_02130"
     CDS             4198..4920
                     /locus_tag="PLH96_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPB60597.1"
                     /translation="MAKLIFDIETAGESYDEMDETTREILTKWIKESCENEEEYEREL
                     KKIKDGLGFSPLTGYIVAIGLLDYEKNKGVVYYQAPDSSEEETEEGDFKFKPMSEKEM
                     LERFWHGAKEYDEFIDFNGRSFDVPFLIARSAVNKVKITKDLMSNRYLNSQRYDAKHV
                     DLFDQLSFYGAVRGKNSLHLWCRALGVKSPKGDGVCGDDVSRLFREKKYLDIARYNVG
                     DLVATRDLYDHWQKYMRAENKQ"
     gene            4917..5678
                     /locus_tag="PLH96_02135"
     CDS             4917..5678
                     /locus_tag="PLH96_02135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HPB60598.1"
                     /translation="MTKDERKYLDWLKKDKYDGQARAASLAVDLGRLRAGEPLDYVIG
                     WRPFLGCKIVLTERPLIPREETEYWAKEAIEEIKKDKRPKIKCLDIFTGSGCVGLAVL
                     ANAKNTVVTFADKSPRALKSVRKNLKVNSFQIGSHRAKVVESDVFSGLSDQFDYIFAN
                     PPYIPLGRKLPESVSRYEPREALRAGRDGLLFIKRFLVQAPAHLVEGGKIYLEFGYGQ
                     KKAVDILLKKIGFGSWVFGKDQFGRCRFAIIKNSR"
     gene            5844..6578
                     /locus_tag="PLH96_02140"
     CDS             5844..6578
                     /locus_tag="PLH96_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPB60599.1"
                     /translation="MNNDNFKNSTISLGLILGISLIISTLIGAYSFYRVRSFDNALSV
                     TGSAKQAVVADSVKWIASFNRTVELGNMKSGYDQMERDLVAVKKFYLAKGFKDENLNI
                     SPVFMDEVWDPNNRGDAKRYVLRRTVEVSSNEVERISEVANNNGELIEAGVIFSTQSL
                     QYFYSKLPDLRVSLLASAIADAKARAGEIAKAGGKKVGDLKAASSGVVQVLSENSVEV
                     SDYGAYDTSNINKEVMVTVKATFYLR"
     gene            6646..7212
                     /locus_tag="PLH96_02145"
     CDS             6646..7212
                     /locus_tag="PLH96_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB60600.1"
                     /translation="MKEEEKIRDLVNKAQNGDDHAFAEIYGRYFTPIYRFIYFKVGNR
                     EEAEDLTQTVFMKVFKSLDRFRFRDNPFSSWLYRIANNMVIDFFRKKKSLPLLDENPE
                     DWQLEFADDALNPVEEAEELELAWSARQAIAEMKDPDRQVLVMRYIDGLSYKDIAETI
                     GKTEEAVRQISSRSIRALKMIFKNKGLL"
     gene            7217..7630
                     /locus_tag="PLH96_02150"
     CDS             7217..7630
                     /locus_tag="PLH96_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60601.1"
                     /translation="MENEEKKLNKMFAVLRQASRPRPELLRRILADLNKPVSSPFFFT
                     DFIKRRSFLSFSVMTMAVLFLVVFGQNLGPAGDPFADSQSVHAQIIREVDKVIESGFA
                     GELEILAAEDALAAEIGTDDFLQDLNSLENELLLP"
     gene            7611..8474
                     /locus_tag="PLH96_02155"
     CDS             7611..8474
                     /locus_tag="PLH96_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60602.1"
                     /translation="MNYYCLKKFLIFIFVFLPVVNFAPLALAAEEAMAFSLPNFLQFS
                     ALAPTPNGTTSTTTRLASTTIATSTATTTEFTPEEFCQKLDEAVTKLEASYSERRQAI
                     SANIDEQNKKLQEERQKYDDQRSWLRTRQDYYLKVYFMRLSQLAETEIQEKGIEQYKK
                     DVVAALAERRALTDLALEEYRQDLDNLKMSKITEMETRLIGDEEKLNGEIQKAAETCE
                     VGDVIKAENSLQNRLFKLRQEREKIFKDVNGAGRAIIAERDRKVAEAEVKYKKAVELA
                     KENLVKFLTAE"
     gene            8602..>9684
                     /locus_tag="PLH96_02160"
     CDS             8602..>9684
                     /locus_tag="PLH96_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB60603.1"
                     /translation="MSDENKQSTNRSKAYILWFKDLSIVDVPLVGGKNAALGEMVNNL
                     MPLGVRLPDGFAITAHAYNYFLTRTNLKEKIKNILEGLDTHNVADLQKRGALIRGMIL
                     KEDIPSDLEAEILAAYRQMSEQLYQTKNVDVAVRSSATAEDLPGASFAGQQETYLNVS
                     GEKDLLKSVKKCFASLFTNRAISYRVDKGFSMFDVLLSVGVQRMVRSDLGSSGVAFSI
                     DTETGFNKVVVINSAYGLGEMVVKGEVIPDEFVVFKPTLEKGYKAILSKTLGEKHEKM
                     IYGKTGTLKVKVKENDRDKFSLGDDQIIQLATWVTLIEKYFSKKYGRYQPMDVEWAKD
                     GKTGELFIVQARPETVHAEADPNLYRE"
BASE COUNT         2728 a         1900 c         2324 g         2732 t
ORIGIN      
        1 cctgtcaggg tggggcgatt ttctttttct tcggtctttc ggtataatct caatatgtct
       61 gaacaaaccc tctatcgcaa gtatcgccct caaaaattta gcgaggttct gggacaagaa
      121 caagtaacgc aagttttgga gacttcgctc aaaaatggcg gcttagttca tgcctatctt
      181 ttttccggcc cgcgcgggac gggcaaaacc agcgtggcca gacttttggc caaagctatc
      241 aattgttctg aacgggactt gtttgaaata gacggcgctt ctaatcgcgg gattgatgaa
      301 attcgcgctt tgcgcgaggc ggtcagcacc ttgccgtttg agtcgcccta taaagtttat
      361 atcatagacg aggtgcatat gctgacgaag gacgctttca acgcccttct caagaccttg
      421 gaagaaccgc cggcgcacgt tgtctttatt ttggcgacaa ctgaaccgca caaggtgatt
      481 gaaacaattg tttcgcgttg ccaaactcat caattcaaaa aaccgactct ggaaatttta
      541 caaaaaatgg tggttgatgt tgcccaaaaa gaaaaggtta gcttggccga agaggtggct
      601 tctttagtcg cttttcttgg tgatggttct tttcgcgata ccttggggat tttgcagaaa
      661 gttatcagtt attcgccaga caaaaaaatc tccgtggagg aggcgcggca aattaccggc
      721 gcgccttcat ttgaattggt gtctgatttt gttttagctt tagctcagtc tgatctggaa
      781 agcgcgttag ccagtttgga taaggtcgct gaacaatctt tggaaatgaa agtttttctt
      841 aagatagcaa tgcgcgaaat tcgtttcctt attttgtcgg tcttcgcgcc tcgcctaaaa
      901 acaaaagtgg ccggggagtt aacagtggcc agtcggacat ttttaaataa accaaaagaa
      961 gaggtggtaa ccggcgccaa acaattagcc tctgtcttgc gcgaattcct ggatatttac
     1021 agcgaactta acgacagctt tattcctcaa gcgccgatag agcttgctct aataaaaacg
     1081 ttgagccaaa agccgtaaaa gcggtttgcg atttgcttgg gtttagcttc tcagtatctc
     1141 agtctcaaac cattgctctt ggaatctcgt cacccttatc aataccatta catcccagca
     1201 cttctgccag acttgttcca atttcaccca ttccaattac taaatgtttt gtcataaaaa
     1261 ttacttcaac agcttgtcgc tggtaacttc cagtgcaacc gcaatccgct caatggttgc
     1321 aagagttgga tttttcttgc cactttccac attactcata tacgcccggt caagtttgag
     1381 cagacgacac aaatcgccct gtgtcatgcc attttcaaga cgtatcctgc ggatattctc
     1441 acccaatctt ttagccgcat cactcatatc tacaactaac agtatacgcg tatttgtctt
     1501 ttgagacaac ggtgttctat agaacacatt ttcgtagcca aaaaccacct gggactcgaa
     1561 ctgagaaaac cctttttaaa tatgggtatt ctaattttgg gcgaaaacag caaaatttcg
     1621 agcccatgac tcgttcaaaa actacgttgc aaaatatgac gattttagga gactcggtcg
     1681 gtctcacatg actgctgcgg gacagggatt cgaaccccga tactcgcctc caaagggcga
     1741 tgtcctacca ttagacgatc ccgcaattac ttgttcattt tgccgtaaaa gagatgtttt
     1801 ttcaagtttt tgagtgccct taatttttaa gagggaaaaa agggagcgaa aaaattcttt
     1861 tttggagtga agtttttgct gacccgaatt gcaaaagtgg caagcggtgt taaaatgtaa
     1921 gtaagtttaa cattaatttt tcaaattttt taaatgaaaa aattaaccta ttttttgatt
     1981 gttgtttgtt tggcagccgc cggctggctg gtctacagtt attattttag caatgacccg
     2041 gtcgcggtaa ctaagaagtt ctatggcggt tggcttgccg gtgattatcg ccttagcgat
     2101 agttcatata aaaatattga agcttggaca gaccaaatga aaaagaaagc tgagggtctt
     2161 gcggcttctt ttaatcaagg agatgatgat ccgattttgt gcgctcaaga caaaccggac
     2221 aactttttgg cctacgaggt gggaagcaat gaagagggtg ttcgggtgga ggtgcgagaa
     2281 gatttttccg gcacggttaa aacagttcag gtctctttga taaaagttgg cggcaaatgg
     2341 aaaattgacg acattgtctg taacgataaa gccgcgccaa gtaatgacga agaacaaaac
     2401 ttggttagcg attatatcaa agaaaatatc tcggagcttt cgccggaaaa ggcggttttg
     2461 ggcgggacct tctccgtgac gagcctcgtc tttggcgaag ggcagactgg tgttgtggag
     2521 tatgaggatg gtcacatttt gttgcgcgcg gggtttaaat attccattgg tgaaaacaac
     2581 gtcgtcaaaa ttgaatcttt tgaagtcttg ccggaggaaa gcggcgcttt ctcggaaact
     2641 ggaaatcttg tcaaagatga aggggcggat ggctggctat tagtttatga aaagcccggg
     2701 caaccggctt tgcgggtgat gttggaattt ggtccgaata gtcgttgcca ggcgactgac
     2761 ggtccaatta cttgcagtga atgggaaatt ggcgaccgag caaaaattga cggtcgtcgg
     2821 caaggcaaca aggtggctgt tgacctggca atagaagaga gaaattaaat caaaaatcaa
     2881 aaatagaaag gcgggtgaaa acagttgttt tcacccgcct ttcacagtca ttggccacaa
     2941 taaactgatg actgaaaaaa gttatagatt agagatgata ttattggctt ttgttttaat
     3001 ccttttgatt ttattgactt atggttatgg gatgtttttc ttaaattttt attgggggca
     3061 atcggttgtt ctctcaccgg ttgaagcgga agaattggcc gaaccgccgt ttgaagaagc
     3121 gatttactcg ccggcgccac ccgggcgagc gcaatttatt tcacccttca gtccgactgg
     3181 cggtttggga gcgccacccg attatcctaa attaatcagg tttggtgaca accaaccgaa
     3241 atttgtaatg tccggcgaac gtcaagttta gtctgtttgt ttgcccagac atagctcaga
     3301 cttaagattt agatttaatt taacctaaac tcagcttatg aattttcgtc aatgacggtt
     3361 gctttaataa ccctgactgt ttcaagcggt ttgtcatttt ggccaacttt cacttggccg
     3421 attttatcaa ctacttcttg ccccttgata actttgccaa aaatagtgta atcgtgcggc
     3481 agcggtgtgt cagccagcat aataaagaac tgactgccgt tggtgtttgg accggcgttg
     3541 gccatggcga caacgccttt ttgatagccg gcctggtaag aagttgtttc cgggtttaat
     3601 tcatctttaa attgataacc tggaccgcct gtgccgcaag ggccggtggc cggacgcttg
     3661 gatagttccg gtgaacagtt tgggtcgcca ccttggatca taaaaccatc aatgacgcga
     3721 tgaaaaatca gcccatcata ataaccttcg ttggctagtt ttataaagtt agcgacggtt
     3781 tcgggcgcgt cggctgggta ggtttcaaat tggatttcgc cgaggttggt ttccagctta
     3841 ataatagatg atgtgttaac ggtcggtgac ataggtttgt ttttatcggg ttctgttaaa
     3901 ttgtcgcttt gactaaggct ataaattacc ataaccagaa ttattaagcc gataataata
     3961 gccaaaagac tgtattttcg ccccataatt tttaattatt attgctaatg tttttattat
     4021 aacagatttt tttgtttggt tttagttctg ccgccaagcc attgagcgtg gatgtcatta
     4081 gacttatttt gttagaaact gcgggcacgt ctaagaccgc gtttgttctt ggggataatt
     4141 tttgaccttg tttcctctcg tgctagaatt aaacaaatta acattatcta caaaaaaatg
     4201 gctaaactta tttttgacat tgaaacagcc ggcgaaagtt atgacgagat ggatgagaca
     4261 actagggaaa ttctaaccaa gtggattaaa gaaagttgcg agaatgaaga agaatacgaa
     4321 cgtgagttga aaaaaataaa agacgggttg ggcttttcac ctttaaccgg ttatatcgtt
     4381 gccattggtc ttttggatta cgaaaaaaac aaaggggtgg tttactatca agcgccggat
     4441 tcgtcagaag aagaaacaga agagggggac ttcaagttta aaccaatgtc cgaaaaagaa
     4501 atgttggagc gtttttggca tggcgccaaa gagtatgacg agtttattga ttttaacggc
     4561 cggtcatttg atgtgccgtt cttaatcgct cgttcggcgg taaataaagt caaaattacc
     4621 aaagatttga tgtccaaccg atacctgaat agccagcgtt atgacgccaa gcatgtggat
     4681 ttgtttgacc agctttcttt ttacggcgca gtcagaggca aaaactccct tcatttatgg
     4741 tgccgcgcgt tgggggtcaa aagccccaag ggagatgggg tttgcggcga tgacgtgagc
     4801 agactttttc gcgaaaaaaa gtatttggat atcgcgcgtt ataacgtcgg tgatttggtg
     4861 gctactcgcg atttgtatga ccactggcaa aagtatatgc gagctgaaaa taagcaatga
     4921 caaaggacga acggaaatac ttagattggc tcaaaaaaga caaatatgac ggccaggcaa
     4981 gagcggccag cctcgcggtt gatttgggtc ggttgcgcgc gggcgaaccg cttgattatg
     5041 tcattggttg gcgtccgttt ttgggttgta agattgtttt gactgaacgg ccactcattc
     5101 cgcgagaaga aactgaatat tgggccaaag aggcgattga ggaaattaaa aaagacaaac
     5161 gaccgaaaat aaaatgcttg gacattttta ccggttccgg ctgtgttggg ctggcagttt
     5221 tagccaacgc aaaaaacacg gttgtaactt ttgccgacaa aagtccgcgg gcgttaaaat
     5281 cggtcagaaa aaatttaaaa gtgaatagtt tccaaatcgg gtcccatcgc gccaaggtgg
     5341 tggaaagtga cgttttttcc ggtctctccg accaatttga ctatattttt gccaacccgc
     5401 cttatattcc gctgggtcgg aaattgccgg agtcggtttc gcgatatgag ccgcgcgagg
     5461 ctttgcgcgc cggccgagat ggtctgctat ttatcaagcg gtttcttgtc caggctcctg
     5521 ctcatttggt ggagggtggt aaaatctatt tagagtttgg ttatggccaa aaaaaagcag
     5581 ttgacattct tttgaaaaaa atcggttttg gttcttgggt ttttggcaag gaccaatttg
     5641 gtcgttgccg ttttgcaata atcaaaaatt cacgctagaa taataaaaag aagctatgaa
     5701 aaattacgcc aaagtaggag gcaaaatcaa ccggcccggt tatcggacgc catccacttt
     5761 taaaatcaag cagaaccaaa aagttgaaaa acggcgaatt cgtaatttta aaccaaaata
     5821 aaaaactaat aatttaaaaa attatgaata acgacaactt taaaaattct acaatttcac
     5881 tcggtctcat tttgggcatc agtttgatta ttagcactct aattggagct tattcttttt
     5941 atcgggtgcg atcgtttgat aatgctttgt cggtgaccgg ttcagccaaa caggcggtag
     6001 tcgctgattc ggttaagtgg attgcttctt ttaaccgaac agttgagctt ggcaatatga
     6061 agtcgggtta tgaccaaatg gagcgcgacc tggtagcggt caaaaagttt tatttggcca
     6121 aaggttttaa ggatgaaaat ttaaatatct ccccggtttt tatggatgaa gtttgggacc
     6181 ccaataatcg cggcgatgcc aagcgttatg ttttgcgtcg gacggtggaa gtcagttcaa
     6241 acgaagttga acgtatttcc gaggtggcaa acaacaacgg agaattaatt gaagccggcg
     6301 ttattttctc tacgcaatca ctacaatatt tttattccaa actaccagac ctgcgcgtat
     6361 cgcttttggc ttccgccatc gccgacgcaa aagcgcgagc tggtgaaatt gccaaagccg
     6421 gcgggaagaa ggtcggtgac ttaaaggcgg cctccagcgg agtggtccaa gttttgtctg
     6481 aaaactccgt tgaagtttct gactatgggg cttacgatac ctcaaacatc aataaagaag
     6541 taatggttac ggtcaaggcg accttctatt tgagatagac taatctttat tttcttgcta
     6601 tttttaagcg ctagagtgtc acataaggac acttaaaacg ttattatgaa agaggaggaa
     6661 aaaatcaggg acttggtaaa taaggcccaa aatggcgatg accacgcttt tgccgagatt
     6721 tacgggcgtt attttacgcc catttaccgc tttatctatt ttaaggtggg caatcgcgaa
     6781 gaggccgaag atttaaccca aacggttttt atgaaggttt tcaagtcgct tgataggttc
     6841 cgcttccgag acaacccctt tagctcgtgg ctttatcgga ttgcgaataa tatggtcatt
     6901 gatttcttcc gtaaaaaaaa gagtttgccg ctcttggacg aaaatccgga agactggcaa
     6961 cttgagtttg ccgatgatgc cttgaaccca gttgaagaag ccgaagagct ggaattggca
     7021 tggtcagcga gacaagcgat agccgaaatg aaggaccctg accgtcaagt gcttgtaatg
     7081 cgctatattg acggtttgtc ctataaagac attgccgaaa caatagggaa aacggaggag
     7141 gctgtcagac aaattagttc gcgttccatt cgggcgctca agatgatttt taaaaacaag
     7201 gggctattat agaatcatgg aaaacgaaga gaaaaaattg aataaaatgt tcgccgtctt
     7261 gcgacaggcg tccaggccgc gaccggaact tctccggcgg attttggctg atttgaacaa
     7321 gccggtatcg tcaccattct tctttactga ttttatcaag cgtcgcagct tcttgtcttt
     7381 ttcagtgatg actatggctg ttctcttttt ggttgttttt ggtcaaaatc tgggtccggc
     7441 gggagatccg tttgccgata gccaatctgt tcacgcgcaa attattcgcg aggtggacaa
     7501 ggtgattgaa tctggttttg ccggcgagct tgagattttg gcggctgaag acgccttggc
     7561 ggccgagatc ggaacagatg attttttgca agatttaaac agtttggaaa atgaattatt
     7621 attgccttaa aaaattttta atatttattt ttgttttctt gccagttgtc aattttgctc
     7681 ctttggccct ggcggctgaa gaggccatgg ctttttcgtt gccgaatttt ttgcaatttt
     7741 ccgcgcttgc tccgactcca aatggcacaa cctcaaccac cactcggctt gcctcaacga
     7801 ctatcgcgac tagcaccgcg acaacaaccg aatttacacc tgaagaattt tgccaaaaac
     7861 tggatgaggc ggtcactaaa ttggaagcca gctattctga gaggcggcaa gccatcagcg
     7921 cgaatattga tgaacaaaac aaaaagcttc aagaagaacg gcaaaaatat gatgaccaac
     7981 gttcttggct tagaacgcgt caagattatt acttgaaggt ttactttatg cgcctcagtc
     8041 agttggccga aactgaaata caagaaaaag ggatagaaca atacaaaaaa gacgtcgtgg
     8101 ctgctttggc agagcgtcgc gctttgaccg acctggcgct ggaggaatat cgccaagatt
     8161 tagacaatct taagatgagc aagattacgg aaatggaaac tcgtctgatt ggcgatgaag
     8221 aaaaattaaa tggagaaatt caaaaagcgg ccgaaacttg cgaggtgggg gacgtaatta
     8281 aggcagaaaa cagtttacaa aatcgcttat tcaaattgcg tcaagaacga gagaaaattt
     8341 ttaaagatgt caatggagct ggacgcgcga ttatcgctga acgcgaccgc aaagtcgcgg
     8401 aagctgaagt taaatacaaa aaggccgtgg aattggcgaa agaaaatctg gtcaaatttt
     8461 tgacggcaga ataaaaaaat gtctaaaagt aaccgccggg tcaacacgtt gacccggcgg
     8521 ttacttttat ttttgaaatg ggttataata aagagaatta ttagccttaa gaaatttttt
     8581 aacaattaat atcagtattt tatgtctgac gaaaacaaac agtcaaccaa tcggtcaaag
     8641 gcgtatatct tgtggtttaa agatttatca atcgttgacg tgccattagt tggcggtaaa
     8701 aatgccgctt tgggagaaat ggtcaacaac cttatgcctt tgggggtgcg tttgccggac
     8761 ggctttgcca ttaccgctca cgcttataat tattttttga ccagaaccaa cttgaaggaa
     8821 aagataaaaa atattttaga aggtttggac acgcacaatg tggccgactt gcaaaaaaga
     8881 ggcgccttaa tcaggggaat gattttgaaa gaagatatac cgtctgattt agaggctgaa
     8941 attttggccg cttatcgaca aatgtctgag cagctttacc aaaccaaaaa tgtggatgtg
     9001 gccgtgcgtt cttcggcaac cgcggaagac ttgccgggcg cttcttttgc cggccaacaa
     9061 gaaacttacc ttaatgtttc cggcgaaaaa gatcttttga aatcagttaa aaaatgcttt
     9121 gcctcgcttt ttacaaatcg ggctatttcc tatcgagtgg acaaaggctt ttctatgttt
     9181 gatgtgctct tgtcggtcgg tgtccagagg atggttcgga gtgacctcgg ttcgagcgga
     9241 gtggcttttt ctattgatac ggaaaccggc tttaacaaag tcgtcgtcat aaactcggct
     9301 tatggtttag gcgagatggt tgttaagggc gaagttattc ctgatgagtt tgtcgtcttt
     9361 aagccgactt tggagaaggg ctacaaggcg attctctcta aaacccttgg cgaaaagcat
     9421 gaaaaaatga tttacggcaa aaccggcact ctaaaagtga aagttaaaga aaatgatcgt
     9481 gacaaattta gccttggcga tgatcagatt atccaactgg caacctgggt caccttgatt
     9541 gaaaaatatt tttccaaaaa gtacggccgt taccaaccca tggacgttga gtgggccaaa
     9601 gacggcaaaa ctggtgaatt gtttattgtg caggccaggc cggagacggt tcacgccgaa
     9661 gccgacccaa acctttaccg ggaa
//
LOCUS       DAOOLO010000024         8867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_9228_length_8867_cov_5.539378, whole genome shotgun sequence.
ACCESSION   DAOOLO010000024 DAOOLO010000000
VERSION     DAOOLO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9228_length_8867_cov_5.539378"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3949
                     /locus_tag="PLH96_02165"
     CDS             <1..3949
                     /locus_tag="PLH96_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60604.1"
                     /translation="NLSQSTRQTAREEAQELSKLQQADFEACQAEAGRGGGMGSGLPG
                     GGSLPGGSGMGSPVGSGGTMGNNEQATREALKSQGIEVKKQPCNGRDYRTVAGGCTDV
                     AGMPQLAVDRLGEMDREMGGGIVLTGGTETGHKTHAPGQSIVDIAMTSQSREYVNKHA
                     VESYPSKYGNGTWYLLDNGDKFLDESDHWHIVFNDKNVPSGSSNNSAILASNQSGFQL
                     VKNKIQSLFAWLGKKIKGISFANIVNADSSVNLGGNLIDPTNISPERLEEIISEMSQA
                     QINRDFSQMNASALSDLLEQMGFNSINTVFDNLSGENINNIVSTLGGETFNDFAHLLV
                     DSSASNIFSNMSVTGFDSLLTGIEDGPLNMLISSLDQTGINNMLTRANDDILNQVFGN
                     LGIGGINNIIAGGSEDLLRGVMNSLGQGNIEQIFESVGISTAGKLLESVGSDFLNEVI
                     PDMSSLGLDNVFGNLGDGNFDQLLGNITGENWEQVFSQLGGVAADDLIGSLGADMMNE
                     ILPTLSDGALNNLFGNIGGDALGMLAEGLSADNLNNIIEDLGAGAINNTLGSLGDKFG
                     DILPNLTDNALGNFLLSGDNSILQAALSGAPEGIVGAALEKLPSGILDQAADIPIVGN
                     LIGGSGGGILGGAASLVTGAGLYVPVVEQQGQLMTATQNTDKQTASIDKTTQEIRDLS
                     IQICTHLRAIRRIQTSFESMKIADEVNANRTRLEALAKYASDVAGKDNPDSLIKTGYK
                     TIIDGNYVNIQAFPKNLDEHLTQIKEEAKQVVLADIRNSNNLYKEELVKLVDDESASQ
                     IDSTVTKEEIEEFRKTDERSAIKTAYAPSFSSRLLAYLPSFISRPLSKLAFGKTASAG
                     GQATATNSSSATDFWNMWFKVTDPENNPYGSAMLAMEAKERAQNIAEAQARDEYLAGQ
                     GILPSGRKCVEEVKDENNKLVACRKWETVAGEGAPGIIVKDTLSSALLLKYNSYLNPG
                     GPESQPPGSAPDAHEPATGGYEPPTSAGKGPGQNNKPGQGTDGQHIPTIDPAKTEQAV
                     KETQESSLGTPTTGGFGGSSGSSGSSPSGSGDIPSPSDSGPNLPDNNGSVLNWSDLLG
                     QLSNLWNRSGDSEDNDSQYIISMLLSLAQNAFRSQPPLIQADAISPTLAEINSGRKPN
                     QVKLTWATPNSTCQTANDWLSRTEDGNFVIVKKTGDDLGHLGSLIVKLPLAASSSLSI
                     TNGDVVNNVAPQKNTSTDKTVVTLSFSLSSVTQNSQISFYLEDKTVKIQANEAKEVLT
                     KLRSEIERLSLNDYNLEHNLDTQTLTVSLKAPTYKLSCKNSNGENIKTINIER"
     gene            3946..6345
                     /locus_tag="PLH96_02170"
     CDS             3946..6345
                     /locus_tag="PLH96_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60605.1"
                     /translation="MIATYSKNKTFIAAILMSLSFALVVIPQFTQAQTLTELPRCTEE
                     QKIFYRNEIARLQKALNEISKVTGSGWQKIDQNAISAETLALLKERNEELKEEFGVDE
                     YLGERSVIYRFTQTGGYGGVVFETQEEIHVEKAKSELGSMLVAQKTSLKKCIQEEDED
                     IINNPTSTPEEISAAQERIQGRQEDLAGDIDEAMAATMADPGSVQSHKLDDSKCFYRW
                     GINPMICLLNIAAAVATIILKLFGVLLWLASSIFDLSIYISIISFKSLIASPGVDAAW
                     RIMRDLANLSFIFILFYIAISVIFDISVGSNGKKLIVDVIIIALLVNFSGFLVRVVVD
                     ASNVVAYEFYSRMSYDPQANDGWVLDKVNANIGTALVSKLGLMNHVVPVENADDPNEV
                     TITRLGHLQIVIGALGGIAIILMASFVLLVAAIMFFIRTVTLLFVYVFSPIGLTLRLI
                     PMFKDAHGKWRDALLKQSFYAPAFLIPLFAVFKILGDKGLSTLAQDAGIGAGLMKISA
                     IQIIIMAMIISCIFIAQKFGAFGTGFATKWAGKGVNLLSKPAVGTTKLLGRGTLDVGK
                     RAGKRIASSGAGKSIAKGASTAGGYIASTRVAQGVTRAAQWTANTAAGKAIGSSVSSG
                     AKNIAKDFKSAVKDKVEHPLLYASDLISSGASKIGGVNNFSIMGSTKEERDKAKEESK
                     KKKEEERNKETNRLLRESLANAANFADVQSIVSGIARDALKIIDLKVFADIPQAACAL
                     SHDQVIRLTNREGKGSDDSKHKSKIRTNILAGGTTPFAFSADPAVYSYMDTGAGRVFW
                     R"
     gene            6357..6920
                     /locus_tag="PLH96_02175"
     CDS             6357..6920
                     /locus_tag="PLH96_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60606.1"
                     /translation="MDNITNEQFNQALKRLPQSAQDFISSKEFNDDLVEVSKKYLLHV
                     NQMGKLSEVIIFTAVGILPTSDFQRNVVKEVGVSGDILNLMIYDINQKIFLPLKKEMY
                     APPKDLISEGDKETEEEPGENQQNRVSEIPEEKEIETPDTFSQKMTAEYGLSREKVEI
                     SDQPNQSEPPQPNNTPPRHDPYREPLP"
     gene            6998..7429
                     /locus_tag="PLH96_02180"
     CDS             6998..7429
                     /locus_tag="PLH96_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HPB60607.1"
                     /translation="MQQFQIPQFIEMEDKLFGPLTFKQFLYLVGGGGICFIIYVWVPL
                     PFIIQIIPIAGVAALALALAFVKYNGRPFIVVLEAFIKYSLGNKLYIWKKDEKRVQKN
                     PTPTQTLTNQNTDLKLPRLSESKLNELAWSLDVKKPHNPEE"
     gene            7431..8075
                     /locus_tag="PLH96_02185"
     CDS             7431..8075
                     /locus_tag="PLH96_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60608.1"
                     /translation="MPPKGKTTQDFVPIKEIRNGVLILKDGSMRSVLMVSSLNFGLKS
                     EDEKNAILFGYQNFLNSLDFPVQIYIQSRRLNIQPYVSVLEQRMIDQTNELLKIQIKE
                     YIEFIKVFTEKYNVMTKSFFVVVPYTPAIFKGGQGEFIAKVTGLFGRKNQKLAVASDN
                     FDENVAQLNQRINVVKGGLSRVGLRADVLGTEEMIELYFKMYNPGEHQTPNIAN"
     gene            8077..>8867
                     /locus_tag="PLH96_02190"
     CDS             8077..>8867
                     /locus_tag="PLH96_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HPB60609.1"
                     /translation="MLPLPFKRKEDKKKDILEPIMPQDIYEMGVLELKDIIAPSALRV
                     TPKSISLGGKVIRTMYVISYPRFLSEEWFSPVINLDTVFDVSIFIHPLDTTTTLRSLQ
                     KKIAEVESQIQERAMKGMVRDPQLDTAQADIENLRDQLQQATEKIFAVGLYITIYADD
                     DNELDKIENEIRSILDSKLVYLKTATYLQEDGFKSVVPFATDYLNVNYKMNSTPLSSL
                     FPFISFDLTSNKGILFGRNLHNSSLVIFDRFSLENYNSVIFAKSGS"
BASE COUNT         2683 a         1956 c         2021 g         2207 t
ORIGIN      
        1 caacttgagc cagagcaccc ggcaaacagc cagggaagag gcccaagaac tttctaaatt
       61 acaacaagcg gattttgaag cttgccaggc ggaggccggg cgcgggggcg gaatgggaag
      121 tggtttaccg ggcggaggca gtttaccggg cggaagcggt atgggaagcc cagttggcag
      181 cggtgggact atgggaaaca atgagcaagc caccagagaa gctttgaaga gccaggggat
      241 cgaggtcaaa aaacaacctt gcaatggccg agattaccgg actgtcgcgg gcggctgtac
      301 cgacgtggcc ggtatgccgc aacttgcagt ggatcgtttg ggcgagatgg acagagaaat
      361 gggtggcgga atagtgctga cgggcggaac agagaccggt cataagacac atgccccagg
      421 gcaaagcatc gttgatatcg ccatgacaag tcaaagccga gaatacgtta ataaacacgc
      481 ggttgaatct tacccttcta aatatgggaa tgggacctgg tatctattag acaacgggga
      541 taagtttctg gacgaaagtg atcactggca tattgttttt aatgacaaaa acgtcccttc
      601 aggctcaagc aacaacagcg ccatattagc ctccaaccag agcggatttc aactggtcaa
      661 aaacaagatc caatcccttt tcgcttggct tggcaaaaaa attaagggga ttagttttgc
      721 caacattgta aacgccgatt ccagtgtaaa tctggggggt aatcttattg acccgacaaa
      781 tatctcgccg gaaagactcg aagagattat ttctgagatg agtcaagcgc agattaaccg
      841 agacttttca cagatgaacg cttcggcctt gtctgatttg ttggaacaaa tgggattcaa
      901 ttccataaac accgtctttg ataatttgag cggcgaaaac ataaacaata tcgtttccac
      961 cttaggcggt gaaactttta atgattttgc tcatttgttg gtagattcct cggccagtaa
     1021 catcttttcc aatatgtcgg tcactggctt tgactcgctc ttgaccggaa tagaagacgg
     1081 acccctgaac atgcttatca gcagtctgga ccaaaccggg ataaacaata tgctgacgcg
     1141 cgccaacgat gatattctta atcaggtttt tggcaacttg ggcatcggcg ggattaataa
     1201 tatcatcgct ggtggttcag aagatcttct ccgcggcgtg atgaacagcc taggtcaagg
     1261 caatattgaa caaatttttg aaagcgttgg tatctccacc gccggcaaat tactagaaag
     1321 tgtcggctcc gattttctaa atgaagtcat ccccgatatg agcagtttgg gtttggacaa
     1381 cgtttttggc aacttgggcg acggcaactt tgatcaactt ttaggtaaca tcaccggtga
     1441 aaattgggaa caagtctttt ctcaactcgg cggtgtggca gccgacgact tgatcggctc
     1501 tttgggggct gatatgatga acgagatttt accaacgttg agtgacggcg cgctcaataa
     1561 cctatttgga aatatcgggg gtgacgcctt gggtatgtta gcagaaggtc tctctgccga
     1621 caatttaaat aacatcattg aggacctagg cgctggcgcg attaataaca ctctgggatc
     1681 gctgggggac aagttcggcg acattttgcc aaacttaacc gacaacgctt taggcaactt
     1741 cttgctcagt ggcgataatt caattttgca agctgccctc tccggcgcgc cagagggaat
     1801 tgttggcgct gcgctagaaa aattgccaag cgggattttg gaccaagcgg ctgatattcc
     1861 gattgtcggg aatcttattg gcggcagtgg cggggggata cttggagggg cggccagtct
     1921 agtaactggt gccggtcttt atgtgccagt tgtggagcaa caaggacagc taatgaccgc
     1981 cacccaaaac accgacaaac agaccgccag cattgataaa accactcagg agattcgcga
     2041 tttaagcatt caaatctgta cccatctgcg cgccatcaga cgaattcaaa ccagttttga
     2101 atcgatgaag attgccgatg aggtaaatgc caaccgcacc aggctagaag cgctcgccaa
     2161 atacgccagc gacgtggccg gcaaagacaa tcctgacagc ttgattaaaa ccggctacaa
     2221 aacgattatc gacggtaact atgttaacat ccaagctttt cccaagaatc ttgatgagca
     2281 ccttacgcaa atcaaagaag aggccaagca agttgtcttg gcggatatta ggaattcaaa
     2341 caatctctac aaagaagagt tggttaaatt agtagacgac gaaagcgcca gccaaattga
     2401 ttccacggtc accaaagaag agatcgagga gtttagaaaa acagacgaac ggagcgccat
     2461 caaaaccgct tacgcgccaa gtttctccag ccgcctattg gcctacttgc caagtttcat
     2521 cagccggcct ttgagcaaac tcgcttttgg gaaaactgct tcagccggag gccaagccac
     2581 cgcaaccaac tcttcctcgg caactgattt ttggaatatg tggtttaagg taaccgaccc
     2641 ggaaaacaat ccttacggct cggcgatgtt ggcgatggaa gccaaagaac gagcgcaaaa
     2701 tattgctgag gcacaagccc gagacgaata tttagccgga caaggaatcc tgccttccgg
     2761 acgcaaatgc gtggaagaag tcaaagatga gaacaacaaa ttagtggcct gtcgtaaatg
     2821 ggaaaccgtg gctggcgaag gcgccccggg aattatcgtt aaggatactt tatccagcgc
     2881 tttattgctt aaatacaatt catacctgaa cccgggcgga ccggaatcac aaccgccagg
     2941 tagcgcgccc gacgcccacg agccagctac tggcggttat gaaccgccca ccagcgccgg
     3001 caagggcccg ggccaaaata acaaaccagg acaagggacc gacggacagc atattccgac
     3061 catcgacccg gccaagaccg aacaggccgt caaagaaacc caagaaagca gtctcggcac
     3121 accaacaacc ggaggttttg gcggcagttc tggttcaagc ggttcaagtc caagtggaag
     3181 tggggacatc cccagcccca gtgattcggg ccccaacctc cccgacaata acggctcagt
     3241 tttaaattgg agcgacctct tgggccaatt gtcaaatctt tggaatagga gcggcgactc
     3301 agaggacaat gactctcaat acataattag tatgttgctt agcttggcgc agaatgcttt
     3361 ccgtagtcaa ccgccactta ttcaagctga tgccatatcg ccaactttgg ctgaaataaa
     3421 ttcaggccgt aaaccgaatc aggttaaact aacctgggca acgccaaact caacctgcca
     3481 aacggctaat gattggttat caagaaccga agatggaaat tttgttattg tcaaaaaaac
     3541 cggggatgat ttgggccatc ttggtagtct aatcgtaaaa ctgcctcttg ccgctagctc
     3601 gagtttgtct attacaaatg gcgacgttgt aaacaatgtt gccccacaaa aaaatacctc
     3661 aaccgacaaa acagtggtga ctttgtcttt ttcgctttcc tctgtaacgc aaaacagtca
     3721 aataagtttt tatttagaag ataagacagt gaaaattcaa gccaatgaag ccaaagaggt
     3781 tttaaccaaa cttaggtcag aaattgaacg cctctctctt aacgattata atctagaaca
     3841 taatctggat actcaaactc tgaccgtaag tttgaaagcg ccaacctaca aattatcgtg
     3901 caaaaacagt aatggcgaaa atatcaagac cataaacata gaaagatgat agcaacctat
     3961 tccaaaaata aaacctttat cgcagcaata cttatgagtt taagttttgc tcttgttgtg
     4021 attccgcaat ttacccaagc ccaaaccttg acagagttac ctcgttgcac ggaggaacaa
     4081 aaaatattct accgaaacga aattgcccgt ttgcaaaaag ctttgaatga aataagcaaa
     4141 gtaacagggt ctggatggca aaaaatagac caaaacgcaa taagcgcgga aacgttagcg
     4201 cttctaaagg agaggaatga ggagcttaaa gaggagtttg gagttgacga gtatcttggg
     4261 gaaagaagcg ttatatacag atttactcaa actggcggtt atgggggtgt cgtttttgaa
     4321 acacaagaag aaatacatgt agaaaaagcc aaatctgaac tcggcagtat gttggtggct
     4381 caaaaaactt ctctaaagaa atgtatccaa gaagaagacg aagatataat taacaatcca
     4441 acttccacac ccgaagaaat aagcgcggcc caagaacgga tacaaggaag acaggaagat
     4501 ctggccgggg acatcgacga ggctatggcc gccaccatgg ctgatcccgg ctcggtccaa
     4561 agtcacaagt tggatgatag caaatgtttc taccggtggg gtatcaaccc aatgatttgt
     4621 cttttgaaca tagccgcggc cgtcgccacg attattctaa aactgtttgg tgtcctgctt
     4681 tggcttgcca gctctatttt tgacctttca atttatattt caataatcag tttcaagagc
     4741 ttgattgcct ctcctggcgt ggatgccgcc tggagaataa tgcgcgacct cgccaattta
     4801 tcattcattt tcattctgtt ttatattgcc attagcgtta tttttgacat ttcggttggc
     4861 agtaacggca aaaaattaat tgttgatgtc attataatcg ccttgttggt aaactttagc
     4921 ggttttctag tgcgagtagt ggttgacgcc agcaatgtgg ttgcttatga attttattcg
     4981 cgaatgagtt atgaccccca agccaatgac ggctgggttc tagacaaagt aaacgccaat
     5041 atcgggaccg cgcttgtgag caagcttggt ttgatgaatc atgtcgtacc ggttgaaaac
     5101 gccgacgatc ccaatgaagt gacaataacc agacttggcc acttgcaaat cgttatcggg
     5161 gcgcttgggg ggatagcaat tattttaatg gcctcgtttg ttttgctcgt ggctgccata
     5221 atgttcttta ttaggaccgt aacccttttg tttgtttatg ttttctcgcc aatcggtctg
     5281 acacttaggc ttatcccgat gtttaaagat gctcacggaa agtggcgaga tgccttacta
     5341 aaacaatctt tttatgctcc cgcttttctc attcctttat ttgctgtctt taaaatactg
     5401 ggggacaaag gtctttctac tctggcccaa gatgccggaa taggagcggg tctaatgaaa
     5461 atatctgcta tccaaattat catcatggcc atgatcatct cttgtatctt tattgcccaa
     5521 aaatttggcg cctttggtac agggtttgcc accaaatggg ctggtaaagg ggttaattta
     5581 ctatctaaac cagcagttgg aacaacaaaa cttttgggca ggggtacttt agatgttggc
     5641 aaaagggcag gtaagaggat cgcttcttca ggagcgggta agagtattgc caagggagct
     5701 tctacggccg gtggctacat agctagcact agggttgctc aaggagtaac tcgtgcggcc
     5761 caatggactg ccaacactgc cgctggaaaa gctattggtt cctctgtgtc cagtggggca
     5821 aaaaatatcg ccaaggattt caaatcggcg gttaaagaca aagtagaaca cccactctta
     5881 tacgccagcg acttaatatc ttcaggtgca tcaaaaatcg gaggagtcaa caattttagt
     5941 ataatgggct ccacgaaaga ggaaagagat aaagccaaag aagaaagtaa aaagaaaaaa
     6001 gaggaagaga ggaataagga aacaaacagg ttgctcagag aaagtcttgc aaatgctgcc
     6061 aattttgctg atgtccaaag tatagtatca ggaatagcaa gagatgccct aaagattatt
     6121 gatctaaaag tttttgcaga tattcctcaa gcggcctgcg ccttaagtca tgaccaggtt
     6181 ataagattaa ccaacagaga agggaaaggg agtgatgata gtaaacataa atctaaaatt
     6241 agaactaaca ttttggccgg cggcactact ccgttcgctt ttagcgcaga tcctgctgtt
     6301 tatagttata tggataccgg agctgggaga gtattttgga gataaaaaat ttaattatgg
     6361 acaatataac aaatgaacaa ttcaatcaag ctctaaaacg cctaccccaa tctgcccagg
     6421 attttatttc atccaaagag tttaacgatg acttggttga agtaagcaaa aaatatcttt
     6481 tacatgtaaa ccagatgggc aaactgagcg aagtgataat ttttacagcc gttggaatct
     6541 tgccgacatc cgatttccaa agaaatgtcg taaaagaagt cggtgtgtca ggagatattc
     6601 ttaacttaat gatctatgat attaaccaaa aaattttctt gccactaaag aaagaaatgt
     6661 atgctccacc caaggattta attagtgagg gtgacaagga gactgaagaa gaacccggag
     6721 aaaaccaaca aaacagagtt tctgaaattc cagaggaaaa agagattgaa accccggaca
     6781 ctttttccca gaaaatgact gccgaatatg gtctgtcacg agaaaaagtg gagatctccg
     6841 accagccaaa ccagagtgaa ccgccccagc cgaacaacac cccaccccgc cacgacccct
     6901 atcgcgaacc gctgccctaa accttatcaa cagtccgcca actaacgcgg gctgttttat
     6961 ttaaagttga actaagttat aattagaaag gaaatctatg caacaatttc aaatcccaca
     7021 gtttattgaa atggaggaca agctttttgg tcccctgacc ttcaaacaat ttctatactt
     7081 ggttggcggg ggaggaattt gttttattat ttacgtttgg gtgcccttgc ctttcattat
     7141 tcaaatcatt ccgattgccg gcgtggccgc tttagccttg gctctcgctt ttgttaagta
     7201 taatggccgg ccatttatcg tggttttgga agcgtttatc aaatactctt tgggaaacaa
     7261 gctctacatt tggaaaaaag acgaaaaaag agtccagaaa aatcccacgc ccacacaaac
     7321 tttaaccaac caaaataccg acttaaaact gccgaggctg tcagagagca agttaaacga
     7381 attggcttgg agtttagacg ttaaaaaacc ccacaaccca gaagaataaa atgcccccaa
     7441 aaggaaaaac aacccaagat tttgtgccca tcaaagaaat ccgcaatggc gttttgattc
     7501 ttaaagatgg ctcgatgcgt tctgtcttaa tggtgtcatc tttaaacttc ggcctaaagt
     7561 ctgaggatga aaaaaacgcc attctttttg gttaccagaa cttcttaaac tcgctggact
     7621 ttccagtgca aatttatatt caatcacgtc gcctcaatat ccaaccatat gtttcagtct
     7681 tggaacaacg aatgattgac caaacaaatg aactgctcaa aatccaaatt aaagaataca
     7741 tagaatttat caaggttttc accgaaaaat acaatgtgat gaccaaaagc ttttttgttg
     7801 tcgtgcctta tacgccggca atcttcaagg gcggccaagg agaatttatc gccaaagtca
     7861 ccggcttgtt tggtcggaaa aaccaaaaac tcgccgtggc cagcgataac tttgatgaaa
     7921 atgtggcgca actaaaccaa cgcatcaatg ttgtcaaggg tggcctttct cgggtcggcc
     7981 tgcgggccga cgtattgggc actgaagaga tgattgaact ttactttaaa atgtataatc
     8041 cgggagaaca tcaaactccc aatatcgcca attaaaatgt tgccattacc cttcaaaaga
     8101 aaagaggata agaaaaaaga cattcttgaa ccaataatgc cccaggacat ctacgaaatg
     8161 ggtgttttgg agctgaaaga catcatcgcg ccgtcagccc tgcgcgtcac tcctaaaagc
     8221 atcagtcttg gcggcaaagt cattcgcaca atgtatgtta tctcttatcc gcgcttcttg
     8281 tcggaggagt ggttttcgcc ggtcattaac ttagacactg tttttgatgt ttctattttt
     8341 attcacccct tagacaccac cacgaccctg cgctccctgc aaaagaaaat cgcggaagtg
     8401 gaaagtcaga ttcaagaaag agcgatgaaa ggcatggtgc gcgaccctca gcttgatacc
     8461 gctcaagccg acatcgagaa tctgcgagac caacttcaac aagcgaccga aaaaatcttt
     8521 gcggttgggt tatacatcac aatttacgcc gacgatgata atgaattgga caaaatagaa
     8581 aacgaaatcc ggtctattct tgattcaaaa ctagtttacc tgaaaaccgc cacttacttg
     8641 caagaagacg gttttaaaag cgttgtaccc tttgcgaccg attacttaaa tgtcaattac
     8701 aaaatgaatt caacccctct ttctagcctt ttcccattta tttcttttga tttgacttcc
     8761 aacaaaggta tcctctttgg tcgtaattta cataactcat cattggttat ttttgaccgt
     8821 ttctccctgg aaaactacaa ttcggtcatt tttgccaaat caggctc
//
LOCUS       DAOOLO010000025         8401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_9926_length_8401_cov_5.721304, whole genome shotgun sequence.
ACCESSION   DAOOLO010000025 DAOOLO010000000
VERSION     DAOOLO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9926_length_8401_cov_5.721304"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLH96_02195"
     CDS             <1..230
                     /locus_tag="PLH96_02195"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HPB60610.1"
                     /translation="FDLAEKDLALRGAGTMSGERQWGISDLGMEAIRNLKMVAAARAE
                     AEKIIESNPDLSLHPNFVKRLGQRKQELHFE"
     gene            complement(487..560)
                     /locus_tag="PLH96_02200"
     tRNA            complement(487..560)
                     /locus_tag="PLH96_02200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Ile,seq:tat)
     gene            complement(640..846)
                     /locus_tag="PLH96_02205"
     CDS             complement(640..846)
                     /locus_tag="PLH96_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017887534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPB60611.1"
                     /translation="MDEESNDDIGSVVAQQYLSGINYPAKKEDLINHAKSQNAPQDVL
                     AMLEDLPDREYGGPNDVASAVDGE"
     gene            1129..1314
                     /locus_tag="PLH96_02210"
     CDS             1129..1314
                     /locus_tag="PLH96_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose starvation-inducible protein B"
                     /protein_id="HPB60612.1"
                     /translation="MTKNRNEDKEKDRMDVEEADRQGDEKMSETHDFDFYSEIGRKSE
                     HRSDSSEDEDEDEETKV"
     gene            complement(1402..3324)
                     /gene="ftsH"
                     /locus_tag="PLH96_02215"
     CDS             complement(1402..3324)
                     /gene="ftsH"
                     /locus_tag="PLH96_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB60613.1"
                     /translation="MAKKRDKGGLSLLTQLGGQLVTVILVLVLVSVIFASLVSEREEL
                     PLIPISEVAQKISNDEVEKIALQGEELLITLKTGAEEKSKKEPEASLSESLVNYGVSP
                     EKLQTVPIEVTNSYGWGFWFLTIFPIVLPILFVIFLFWLISRQMKGASMQAFSFGQSR
                     ARLIDPNDTSQRVTFKNVAGVREAKEELLEIVDFLRSPKKFLDIGARIPKGVILVGAP
                     GTGKTLLARAVAGEAKVPFMYLSGSEFVEMFVGVGASRVRDLFKMAKKVAPSIIFIDE
                     IDAIGRHRGSGLGGGNDEREQTLNQILVEMDGFEPSEKVIIMAATNRPDVLDPALLRP
                     GRFDRRIILDQPDKRDREEILAIHAVKKPLGEDVNLKVVAERTPGFSGADLSNLMNEA
                     AILAARENRRTITQYDLLRSVEKVMLGPERKSHILSVREKKITAYHEVGHALISSLLT
                     YSDPVHKVSIIARGQAAGYTLKLPFEDRRLKSRNEFLDDIAVSLGGYVTEKLIFNDVT
                     TGASNDIQVATALARDMVTKYGMSDELGPVALDSFSHTNLNAGLINERSISEKTFSEI
                     DKEVSKIMNEAKKRAEDVIVKHKTALDNLADELMKMETLEREDFEKLLIVNGIKPKSG
                     PEKEVEVFAPDSVRGE"
     gene            complement(3689..6388)
                     /locus_tag="PLH96_02220"
     CDS             complement(3689..6388)
                     /locus_tag="PLH96_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPB60614.1"
                     /translation="MFKLAKQGFTLVEVLVVVSIISLLLGTLVVNLNKAKDAAKETVA
                     KSNVNEMEKSLDAYYLDHGSYPSAQGGTESSDIGWSLSFVQALTPKYISELPGGPQDE
                     FVYTYSLDSSGQNYKFSYQRKDSSQTSNPSVPTDPSDPIENPTDPTDPGGGLGDSTLT
                     ATANPSSQNFNDSLTVSLVANHPDASISYTLDNSLPSKDSTLYTNPIVLTSNAILKFF
                     AWLNEQESDIYTETYTKTAAAPPVAVITVDEPSIYTPGSSGYAPDWGMTLTKGYAPYP
                     IFFQGWQSTPREDIVKYEWDFGDGSAKFYGFNAAHVYETPGNYTVSLRVLNSAGVWSG
                     YDSINVEIMSRAGKRTYYVDSQLGDDSNSGLCETPDGKGCGPWRTAQKAFASMPRPNQ
                     WNTNISTWTYQPGDRILFKRGQAFEVTSKTTIGNGYGTGGYYFGAYGSGAKPLIQWKG
                     ASSDFMLDFDFGSGYIGFVDLQFQFYDETTGIKGIGLIWVNEYLKNILFLRDDFYNPD
                     NGVWNISGVDKSRPVSGLFMVNSTVHNNETHSTSVTQIAAFGLNSLAILNSTFDQSGN
                     HTAYFGYIDKAVIKDSVFSRPAFGRAALRISGESFEFPANNIYVADNYFLGWVDPVAD
                     SRAHNGGGTRYNYNLVNFGPNNTSEKMLTDIIFENNVVTNSESLMDITNGENIKIRNN
                     IFVTPDVSTARKFAFSSNVGWETRPLKNVDFVGNTFVLSGNVPTYALNGILQINDFDK
                     TSVYGDRHQNVRIMNNIIYSNGSGRAIWFDKLDFSDLAEFTINNNIYYLVNAVQDKLI
                     FVGDTGYTLEQWRTQSGQDINSQATNPKFLNGVSQITHSPGEPSSLSAGIMEANNYKN
                     MVKSAADSPAVDAGANLGAGLYYDLFGTKRPQGAGYDIGAYER"
     gene            complement(6634..6837)
                     /locus_tag="PLH96_02225"
     CDS             complement(6634..6837)
                     /locus_tag="PLH96_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60615.1"
                     /translation="MSIQLLISAKPLFQTTTSLCLRSGHDWSLPVMRGGGLVQVTRTC
                     WRCGQVDERVESEYEAETQASFP"
     gene            complement(7093..7332)
                     /locus_tag="PLH96_02230"
     CDS             complement(7093..7332)
                     /locus_tag="PLH96_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPB60616.1"
                     /translation="MYYVYILKSEKDGSLYKGSTSDIKKRLAEHNFGNSKYSSSKKPF
                     KLIWYCAFPTKKQALEFEIYIKSGSGFAFANKHLI"
     gene            complement(7510..7892)
                     /gene="ssrA"
                     /locus_tag="PLH96_02235"
     tmRNA           complement(7510..7892)
                     /gene="ssrA"
                     /locus_tag="PLH96_02235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7940..8392)
                     /gene="smpB"
                     /locus_tag="PLH96_02240"
     CDS             complement(7940..8392)
                     /gene="smpB"
                     /locus_tag="PLH96_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPB60617.1"
                     /translation="MANLVENKKVRLNYEILEEYEAGMELLGGEVKALKGGQGSLDGA
                     RVIIRAGEAFLLGFHIPPYQPNNATGAYEPERTRRLLLTRKELKELTGRAEQKGLTIV
                     PISVYNKKRWLKVKIAVVRGKKKFDKRESLKKKEAKRDIARDIKGTLK"
BASE COUNT         2481 a         1954 c         1629 g         2337 t
ORIGIN      
        1 gttttgacct ggctgaaaaa gatttggctt tgcgtggagc cggcacaatg agcggtgaaa
       61 ggcaatgggg tatttccgac ttggggatgg aagctatccg caacctaaaa atggtcgccg
      121 ccgcccgcgc tgaggcagaa aaaataattg agagtaaccc agacttaagt ctccatccca
      181 attttgttaa acgattggga caaagaaaac aagagctgca ttttgaataa tctaatgccg
      241 ataagacaca cttgtccacc ttgggcggaa atttacacgg atgaaacttc cttcgcttaa
      301 agctatagag ggcacagcgc cgattcatgc ggattaagca cctttctgca tttgtaaatt
      361 cgtgtctgcc atttgtatca ttagcattgt gcagtgtttt cccgacccct ttcctgttcg
      421 agctcaactg tggtttttct atgttttagt tttgcgcttt gagttttaaa ttttgcgcta
      481 tctttgtgca cgcgcctgga ctcgaaccag ggaccgcgga ggtataagct ccgtgctcta
      541 accaactgag ctacgcgtgc gcgcaacctt tacactttaa aacaaaaacc ggcctttttc
      601 aataagccga tttttgttat cttcgttgct taaatttccc tactcaccgt caaccgccga
      661 agcaacatca ttcggaccgc catattcgcg gtcaggcaaa tcttccaaca tcgccaacac
      721 atcttgaggc gcattttgac tcttggcgtg gttaattaaa tcttcttttt tagcgggata
      781 atttattccg ctcaaatatt gttgcgcgac gaccgaacca atatcgtcgt tactttcttc
      841 atccataaaa atttaattac aaattttgta atttcgactt ttagactttt gaaaaccgtc
      901 ataactatag accggcttaa tgacaataga ctgaagcaaa cgcttcgatt atcagccgcc
      961 aaggccgcaa aagtgacagc caaaacaaaa cttgttacca aagaaacaca gttagtgaaa
     1021 aataatttaa aattaatgcc gcattcatct cgacttttta gactgatcaa actcaaaggt
     1081 cggcccatta atcgttaatt agtcgttaac ctaacaccat aaatttgtat gacaaaaaat
     1141 agaaacgaag acaaagaaaa agacagaatg gacgttgaag aagccgaccg ccaaggcgat
     1201 gaaaaaatgt ctgaaactca cgactttgac ttctattcgg aaatcggtcg caagagcgaa
     1261 catcgctccg atagttctga ggacgaagac gaggacgaag aaacaaaggt gtaagttgag
     1321 actttacaat taacaaaaca aaaaccaagc cgtggcttgg tttttgtttt gttaattgta
     1381 acttttatat cctcattaca gttattctcc cctgaccgaa tcaggcgcaa agacttccac
     1441 ttctttttcc ggccccgact tgggcttgat gccattaaca atcaataatt tttcaaaatc
     1501 ctctcgttcc aaagtttcca ttttcatcag ttcatcagcc aagttatcca acgcggtttt
     1561 atgtttgaca atgacatctt ccgctctttt tttcgcttca ttcattattt ttgatacttc
     1621 cttgtcaatc tctgaaaagg ttttttcgga aattgaccgt tcattaatca gaccggcatt
     1681 caagttggta tgtgagaaag aatcgagcgc tactggcccc aactcgtcac tcatgccata
     1741 tttggtcacc atatcccgcg ccaaagctgt tgccacttgg atatcattag aggcgccggt
     1801 cgtcacatcg ttaaaaatta atttttcggt gacataaccg ccaagcgaca cggcaatatc
     1861 gtccagaaat tcattgcgcg acttgagccg acggtcttca aacggtaatt tcaaagtata
     1921 accggccgcc tggccacgag caataataga aaccttatgg accggatcag aataagtcaa
     1981 aagagacgag attagggcat gccccacttc gtgataggcg gtaatctttt tttcacgcac
     2041 cgataaaatg tggctcttcc tttccgggcc gagcatcact ttttccactg aacgcaacaa
     2101 atcatattga gtaatagttc gccgattctc gcgggccgcc aaaatcgccg cttcattcat
     2161 caaatttgaa agatcagcgc cggaaaaacc cggtgtcctt tcagccacca cttttagatt
     2221 gacgtcttcg cccaatggtt ttttgacggc gtgaatggca agaatctcct cacggtcgcg
     2281 cttgtcaggt tggtccaaaa tgattcgtcg gtcaaaacgt cccggacgga gcagagccgg
     2341 gtccaagaca tcaggccggt tggtcgccgc catgattatc actttttccg atggctcaaa
     2401 tccgtccatc tctaccaaaa tctggtttag agtttgttct cgttcatcat tgcccccacc
     2461 caaaccggaa ccgcgatgac gaccgatggc atcaatctcg tcaatgaaaa taatggaggg
     2521 ggcgactttt ttagccatct tgaatagatc gcgaacgcgt gaagcgccga caccgacaaa
     2581 catctctacg aattccgaac cggaaagata cataaagggc accttggcct caccggcaac
     2641 cgcgcgagcc aaaagggtct tgccggtgcc tggcgcgcca accaaaataa cgcctttggg
     2701 aatacgcgcg ccaatatcca aaaacttttt aggcgaacgc aaaaaatcca caatctccaa
     2761 aagttcttct ttggcctcgc gcacaccggc cacattcttg aaagtcaccc gttggctagt
     2821 atcgttgggg tcaatcaagc gcgcccgcga ctggccaaat gaaaaggctt gcatactggc
     2881 gcccttcatc tggcgagaaa tcagccaaaa caaaaaaata acaaacaaaa tcggcaagac
     2941 aattggaaag attgtcaaga accagaaacc ccagccatag gaatttgtta cctcaattgg
     3001 cactgtttgc agtttttccg gcgaaacacc gtaatttacc aagctttcag acagagaagc
     3061 ttccggttct ttttttgatt tctcttctgc gccggttttt agcgtaatca acaattcctc
     3121 gccttgcaaa gcaatttttt ccacctcgtc attgcttatt ttttgggcga cctcggaaat
     3181 tggaatgaga ggcaattctt ctcgttcgga aaccaatgac gcaaaaatca cggacaccaa
     3241 aaccaagact aaaataaccg tcaccagctg cccgccaagc tgagttagaa gcgatagccc
     3301 acctttgtct ctttttttag ccatttactt attttttatg ctaattatcc ttaagattaa
     3361 tgaaattttc ggcaatttaa agccaaaaaa cgcggttagt ttcactttga ctcctcagtt
     3421 tgtcatttta gcaaaaatat gcaaatattg aaaccataac tagttttgac aatcgttatg
     3481 tttaaaaaag acaacctaca tcgtttaatc tttatccttt ctttccccta ttggtattat
     3541 aaagaatttc tcaaagaacc ggagaaaaaa taaagaggga gcgcaaaaaa ataggcctta
     3601 taaatatttg ggttggttca taaaccaaga agttttaaac caccgaaaaa cgcccttcaa
     3661 cgcagggggt tttcattatt tgcgtgtctt atctttcgta ggctccaata tcataaccgg
     3721 ccccttgcgg acgtttggtc ccaaacaaat cataatacaa accagcgccc agattggctc
     3781 ccgcgtccac ggccgggctg tcagcggctg atttcaccat gtttttgtaa ttgtttgctt
     3841 ccataatacc cgcggataaa cttgagggtt ctccaggact atgagttatt tgacttacgc
     3901 catttaaaaa ttttgggttc gttgcttggc tattgatgtc ttgtccggac tgagttctcc
     3961 attgttcaag tgtgtaacca gtatccccaa caaaaatcaa tttatcctga accgcgttaa
     4021 ccaaataata aatattgtta ttgatggtaa attccgccaa gtcactaaaa tcaagcttat
     4081 caaaccaaat tgccctccct gaaccattgg aataaataat attattcatt attcttacat
     4141 tctgatgacg atcgccataa acagaggttt tgtcaaaatc atttatttgt aaaatgccat
     4201 tcaaggcata agtgggcaca ttcccgctta aaacaaaagt gttgccaaca aagtcaacat
     4261 tttttaaggg tctggtttcc cagccaacgt ttgaggaaaa agcgaattta cgagcggtag
     4321 agacatcagg ggtcacaaaa atgttgtttc ttatcttaat gttttctcca ttagtaatat
     4381 ccattaaact ttcactattg gtgacaacat tattttcaaa aataatatca gtcaacatct
     4441 tttctgaggt attattgggg ccaaaattaa ccaaattata attatacctg gtgccgccgc
     4501 cattgtgagc gcgactgtcg gcaaccgggt caacccaacc aagaaaataa ttgtcagcaa
     4561 cataaatatt attggctgga aattcaaagc tttcaccgct gattcgcaaa gccgcccgac
     4621 caaaagcggg gcgagaaaaa acactatctt taatgaccgc tttatcaata tacccaaaat
     4681 aagcagtgtg attaccggac tggtcaaaag tgctgttgag aattgccaat gaattaaggc
     4741 caaaagcggc tatttgagtt actgaggtgc tgtgagtttc attgttatga acggtgctat
     4801 taaccataaa aagaccggac accggtcgag acttatccac cccgctaata ttccaaacac
     4861 cattgtcagg attataaaaa tcatcgcgta aaaataaaat attcttgaga tattcattaa
     4921 cccaaatcaa accaattccc ttaatgccgg tggtctcatc ataaaattga aattgcaaat
     4981 caacaaaacc gatatatccg ctgccaaaat caaagtcaag cataaaatca ctactggcgc
     5041 ctttccattg aattaaaggt ttggctcccg agccgtaggc accaaaataa tagccgccgg
     5101 tgccgtaacc attgccaatc gtcgttttgc tggtgacttc aaaggcctga ccgcgcttaa
     5161 acaaaatccg gtctccgggt tgataagtcc aagtggaaat attggtattc cattgatttg
     5221 gccgaggcat tgaggcaaaa gctttctggg cggtccgcca tggtccgcaa cccttgccgt
     5281 cgggagtctc gcataaaccg ctattactat cgtcgcctaa ttgtgaatca acataataag
     5341 ttcgcttgcc agcgcgagac attatttcta cattaatcga atcataaccg ctccacacgc
     5401 cagcgctatt taacactctt aagctgactg tgtaattgcc cggtgtctca taaacatggg
     5461 cggcgttaaa accgtaaaat ttggcgctac catcgccaaa gtcccactcg tatttgacta
     5521 tatcctccct tggcgtggat tgccaacctt gaaaaaatat cggataagga gcgtagcctt
     5581 tggtcaaagt cataccccaa tccggagcat aaccgcttga gccgggagtg taaatacttg
     5641 gttcatcaac agttatcact gccactggcg gcgcagccgc ggtcttggta taagtctcag
     5701 tataaatgtc tgattcttgt tcattaagcc aagcaaaaaa tttaagaata gcattactgg
     5761 ttaaaacaat tgggttagta taaagagtag aatctttaga tggcaaagaa ttatcaagag
     5821 tataagaaat actggcatcc ggatggttgg caaccaaaga aacagttagg ctatcattaa
     5881 aattttgaga gctcgggttg gcggtcgcgg taagagtgga gtctccaaga ccccctcctg
     5941 ggtccgtagg gtctgtggga ttttcgatag ggtcagaagg gtctgtggga actgagggat
     6001 ttgacgtttg agaagaatcc tttctttgat aactaaactt ataattttgc ccgctagaat
     6061 caagactata ggtataaaca aactcatctt gtggaccccc gggcaattca gagatatatt
     6121 tgggggtcaa ggcctgaaca aaagacaagc tccagccaat gtcgcttgat tctgtccccc
     6181 cttgggcact cgggtaagaa ccatggtcaa gatagtaggc atctaaggat ttttccattt
     6241 cgttaacgtt ggacttagca acagtttctt tggcagcatc tttcgcttta tttaaattaa
     6301 ccaccaaagt tcccaacaac aaagaaatga ttgagacaac caccaaaact tcaactaggg
     6361 taaaaccttg tttggctaat ttaaacatgc cttttattat atattaaact taactactta
     6421 actacttatt agcaatagtc attcttcatt tttccctttt tcgctttaaa actaagcctg
     6481 atttaaaaac tctaaccact tccagcccca aattgattaa ttttaccgag agccgccttg
     6541 gcgctgtgag aaattaaaat ttggtcaaaa aaatcggatg ccaacagaaa aactgtggca
     6601 cccgagaaga acaaacatcg cttccagtga gagtcaggga aaagaagcct gcgtttccgc
     6661 ctcatattct gattccaccc tctcatcaac ttgaccacaa cgccagcagg tcctcgtgac
     6721 ctgaacaaga ccgccgcctc gcatgaccgg cagagaccaa tcatggccag acctaagaca
     6781 taacgaggtg gtggtctgga acaagggctt ggctgagatc aaaagttgta tcgacatagc
     6841 gctcgtctcc tttagcagtt aagccaaacc aagacctggt cttaggcaaa gctttgaccc
     6901 aaagaaaacc caaagaaaca ttactttttt ctttttattt tttaacttag caacatgaaa
     6961 aaaccaagaa caaagaaaaa aattttggcc cgagccccac gcgagcaaaa cagtttgctc
     7021 gccccactta aaacacccgc tctcgcaggc cgttttttgt gatcgtaaat gacaaaaggg
     7081 aatctcccgc gactagatca gatgtttgtt ggcaaaagcg aaaccagaac cagattttat
     7141 ataaatctca aattctaaag cctgtttctt tgttgggaaa gcacaatacc aaataagttt
     7201 aaaaggtttt tttgaagatg aatattttga attaccaaaa ttatgttcag ccaatctttt
     7261 tttaatatca gacgttgaac ctttgtataa actgccatct ttttcgcttt tcaaaatata
     7321 gacgtaatac atagttaaat tatatcgccc aaatttaaac aaagctacga cctacttagc
     7381 tatcgctatg agcttcgaag ggcccctgct tctatttaaa ccaaataacc tccttactcc
     7441 gtaggaggat tattttgttg cttcttgtgc aagcgagaaa tgtgcttgcg ctgcgaagct
     7501 ttagcgtagc agtggaagtg gggggaatcg aacccccgtg caaatagttt tctttggtca
     7561 atctatatgt ttagaacatt tatttgtttt aaaccaaaat gctcttaaat gaacaaaaaa
     7621 cattttggtc gatttcctgt ttctcgccag tcggacggaa caaaccgact aagccagtct
     7681 gaaagctatt ctgccctcaa aaacctatca gacggttggt ctaagaggac accggcaatt
     7741 acgctaaagc gaaggccgga acaaagtatg gcgaagccac accttgcgtg tcagatgctt
     7801 tggcatttga ctgtttttgg acagttttag gagttatcca aaactccaca tacaaaccaa
     7861 ggagaggcta ctcgtctaag ccaaacactc ccgtataata aaatatattt tgtatatagt
     7921 acattgtaga tactatatat tatttcaatg tgcccttgat gtctcttgcg atgtcacgct
     7981 tggcttcttt tttcttgagc gattcacgtt tatcaaactt ttttttacct cggacaaccg
     8041 caattttaac ctttaaccac ctctttttat tatacaccga aatagggaca attgtcaacc
     8101 ccttttgttc ggctcgaccc gtcagttctt tcaattcttt ccgagtcaat aaaagccgcc
     8161 tagtccgctc cggttcataa gcgccagtgg cgttgttcgg ttggtaaggc gggatatgaa
     8221 aacccaacaa aaaagcttcc ccagcgcgaa ttatgacccg cgcgccgtcc aacgaacctt
     8281 gtccgccctt gagcgccttg acctcgcccc ccaaaagctc catccccgct tcatattctt
     8341 ccaaaatttc ataattcaag cggacttttt tgttttccac caaattagcc atggtttaat
     8401 a
//
LOCUS       DAOOLO010000026         8139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_10357_length_8139_cov_6.776843, whole genome shotgun sequence.
ACCESSION   DAOOLO010000026 DAOOLO010000000
VERSION     DAOOLO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10357_length_8139_cov_6.776843"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..83)
                     /locus_tag="PLH96_02245"
     tRNA            complement(2..83)
                     /locus_tag="PLH96_02245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:taa)
     gene            complement(148..219)
                     /locus_tag="PLH96_02250"
     tRNA            complement(148..219)
                     /locus_tag="PLH96_02250"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Cys,seq:gca)
     gene            complement(338..661)
                     /locus_tag="PLH96_02255"
     CDS             complement(338..661)
                     /locus_tag="PLH96_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HPB60618.1"
                     /translation="MSFHKEKEESLLQSLIAEYLNREAGTKSLITVTHCVITPDSKKV
                     TAYISVIPENLEEEALNFAKRRRPEIRQHIKEHSRLRHLPHVEVVIDEGEKNRQNIER
                     LLREG"
     gene            complement(678..2174)
                     /gene="infB"
                     /locus_tag="PLH96_02260"
     CDS             complement(678..2174)
                     /gene="infB"
                     /locus_tag="PLH96_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPB60619.1"
                     /translation="MAETDKNKQIARPPVIAVMGHIDHGKSKLLDYIRQTNVVERESG
                     GITQHVSAYEVVHKDQKITFLDTPGHAAFEAMRVRGAQICDIAVLIVSAEEGVKTQTL
                     EALKSIKEHNKPFVVAISKIDKPEANPDRTKQSLAENDVFVEGYGGAIPCALVSSTEG
                     TGVDELLDLLLLVAEMEELKGNPEKPAKGFILETNLDPRAGVTATLIIKDGTLSDKDF
                     VLAGNALGKIKKMENFLGKPVKELMFSSPVKVFGFSELPPVGCEFRAFAAKDEADKWL
                     AENPFTIAPCGKIFNPHVAENAIRIPLVIKADVAGTLEAVEKELAKINLERVFLEVVA
                     KNVGPVSENDVKLVSAFPEAVILGFKVKSEKSAITLAEKSGVTIKTSDIIYKLSEWLE
                     ELALGRTPKVTTEETIGRAKILKTFAATKDKQIIGGVVSAGKIVKGLKVKIHRHDNEI
                     GQGKILDLQIQKIKVNEVEENTQFGAEVEAKIEIAGGDVIYAFETVTK"
     gene            complement(2273..2740)
                     /locus_tag="PLH96_02265"
     CDS             complement(2273..2740)
                     /locus_tag="PLH96_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HPB60620.1"
                     /translation="MDINTLLVVTLGLIAIVIFVLVRMVILEKRLKSLLSGKNARDLE
                     DTIAGNVVTIQKMDKRILLIAQEIDNINKRLAKAIQKVHTVRFNPFKDQGGNQSFATS
                     FLNEQGDGVVISSLYSRDKVSIYAKPISDGKSEYELTQEERAAIERAEQQTSS"
     gene            2769..3959
                     /locus_tag="PLH96_02270"
     CDS             2769..3959
                     /locus_tag="PLH96_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPB60621.1"
                     /translation="MPTMELPFKSGKTRHYWDTAAATPLAPIVERAMVKTAKDFGNPA
                     SIHREGVLAQEKLAAARQTVARLIAAEPDEIVFTGGGTEGDNLAILGVARARSQVGHL
                     ITTTVEHKAVLEPCRYLADLGHQISYLPVSADGLVNLKEVEKTLTTETFLVSIIYANN
                     EIGTIQPIKEIAKVVRRWRKEHKTNLPYLHIDACQAPRFLNLNVLQLGVDLMTVNGSK
                     IYGPKGVGFLYVKRGVRLLPFLLGGGQEEGRRSGTHNLPAIVGLAKALEICEAEKEAE
                     TEKLTAIRDYLATQLTKIAGLKLNGGFGKNERLPNNLNFSVEGLEGEQLVLELDARGF
                     AVSSGSACSFGADDGSHVVAALGYGEARALGAVRVSLPRETKLAEAKKFVEKVKEILK
                     KYKG"
     gene            4012..4815
                     /locus_tag="PLH96_02275"
     CDS             4012..4815
                     /locus_tag="PLH96_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPB60622.1"
                     /translation="MNINDLNKSQFFLLILLVMFVTSVTTAIVTVTLLDKSPSGGFSG
                     AVTQVVEKTIEKIVPGATTTIVKIIREAPLPNEGELIAQAVNKVSPSVVGVSQKDKDS
                     NKKLGAGFVLSNDLVVTGAKILPPDIKELSVSFGKANLQADLISRDDNHGVAFFRINA
                     SGTEIVQTGQLAEEGPTVGQTVVGLSVASDGSAEIATGIVIDFVSAAATSTNQTGTST
                     AGLIRTNAVNSDNVGGPIIDKQGKIVGIGIARGYALAADSLKNLIDQIK"
     gene            5014..7710
                     /locus_tag="PLH96_02280"
     CDS             5014..7710
                     /locus_tag="PLH96_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB60623.1"
                     /translation="MPPFQNFTSKAREAIRKSHELAIERGQNQVNPIHMLAALVLQDE
                     SLVVSIIEKLEVDSIALTDYILDSIEGPGMGSVVSPNYQIYLTPELVRVFEESIKQAG
                     SLRDEFVSTEHLLLAILDIPSQAKEILNRFKITRDNVFRILEELKSGKMPNLTETKKF
                     KAIEKFTRNLTEMARADKLDPVIGRDGEIRRIMEILSRRTKNNPVLVGEAGTGKTAIV
                     EGLAIRIAKGDAPESLRGKEIVSLDLGLLLAGTKYRGEFEERLKSIIKEVEKSENKII
                     LFIDEIHTIVGAGAAEGAIDASNMLKPALARGDLRTIGATTIKEYQKYIERDQALTRR
                     FQPVLVEEPTVEDAITILRGLKDKYELHHGVHITDDSIISAVNLSARYITDRFLPDKA
                     IDLIDEAASALRLQLENKPAVLEDNERKIMRLEVEKEALKKEVSLNDKNKNAKTRIKE
                     IDTEIAELKEKVSELELRWKNEKQLINEIRDIKEELEKLKFEASQAELRADLGKAAEI
                     LYGRVPELQKNLKMRENKLKKLQATRKVLKEEVAEEEVAKVVSRWTGIPVTKMLEEES
                     EKLNRMEEELRKRVKGQDEAVEKVSESVRRSRAGISDPDRPIGSFIFLGPSGVGKTEL
                     ARGLAEFLFNDEKALIRVDMSEYMEKYSVSKLIGSPPGYVGHEEGGNLTELVRHRPYS
                     ILLFDEVEKAHPEVFNILLQVLDNGRLTDSKGRVVNFKNTIIIMTSNIGSQYIKNFNK
                     IGFSGRDKAEAGFEDVKEKMMDNLKDHFRPEFLNRLDEIIIFNPLSEEVLKQIVDIQL
                     GLVKERLMKKGVFLDVSEEAMFYLAKEGFNPEYGARPLKRLIQSKILNPVAKFIIARS
                     VDNGGTVTVEMKEGEPAVELKKVFRRRASNVRGDSKTSASKK"
     gene            7768..7850
                     /locus_tag="PLH96_02285"
     tRNA            7768..7850
                     /locus_tag="PLH96_02285"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Tyr,seq:gta)
BASE COUNT         2209 a         1609 c         2065 g         2256 t
ORIGIN      
        1 gtgcccgggg cgggaatcga acccgcacgg atttctccac acgattttaa gtcgtgcgtg
       61 tataccaatt ccaccacccg ggcttgaaag atgacaaaga gaaataaaaa tggaaaagag
      121 ccgacacaaa ctaaaaaagt ttgtttgagg cctggggcgg aatcgaaccg ccgtataagg
      181 gttttgcaga cccttgcctt accacttggc gaccaggcca ttcttaatat gaaaagaaaa
      241 gattaaaatg taaatagtcg gaataaatgg aaaaatcgcc gcgttaattt tagcactttt
      301 tgtttctttt ttctattgtc aaaaaacacc gtctttctta gccttctcgc aacagcctct
      361 caatgttttg acggtttttc tcgccttcgt ctattaccac ttccacatga ggcaagtggc
      421 gcaggcggga atgttctttg atgtgttgac gaatttccgg ccgacgacgt ttggcaaagt
      481 tcagcgcttc ttcttccaaa ttttccggaa taactgaaat ataggcggtc acttttttag
      541 agtctggcgt tatgacgcag tgagtgacgg taatcaatga tttggtgcca gcttcgcgat
      601 ttaggtattc ggcaattaag ctttgcaaaa gtgattcttc tttttctttg tgaaaagaca
      661 tgggagtttc gcgtttttta ttttgttact gtctcaaaag cgtaaatgac gtcgcccccg
      721 gcgatttcta ttttagcttc aacctcggcg ccgaattggg tgttttcttc aacttcgttt
      781 actttaattt tttgaatttg caaatcaaga atttttcctt gaccgatttc gttgtcgtga
      841 cgatggattt ttacctttag gcctttaaca attttgccgg cgctaacgac gccgccaata
      901 atttgtttgt ctttggtggc ggcgaaagtt tttagaatct tggcccgacc gattgtttct
      961 tccgtggtta ctttgggggt tcgcccaagc gctagctctt ccaaccattc tgataactta
     1021 taaataatat cagaagtttt tatggtgacg cctgattttt cggccaaggt gatggcgctt
     1081 ttttcggatt tgactttgaa cccaagaatg accgcttcgg gaaaagcgct gaccaactta
     1141 acatcgtttt cagaaactgg cccaacgttc ttggccacaa cttctaaaaa gacgcgctcc
     1201 aaattgattt tggcaagttc tttttctacc gcttccaacg tgccggcaac atcagccttg
     1261 ataacaagcg gaattcggat ggcgttttca gcgacgtggg ggttgaaaat tttgccacag
     1321 ggggcaatcg taaatggatt ttcagccaac catttgtcag cctcgtcttt ggcggcaaaa
     1381 gctctaaatt cgcaaccgac cggtggcagt tcagaaaagc caaaaacttt gactggggag
     1441 gagaacatta gttccttgac cggcttgccc aaaaagtttt ccattttttt aattttgccg
     1501 agagcgttgc cggccaagac aaaatccttg tcgcttaggg tgccgtcttt gataattaga
     1561 gtggcggtca caccggcgcg cgggtccaga ttagtttcca aaataaaacc cttggctggt
     1621 ttttctggat tgcctttcaa ctcttccatt tcggcgacta aaagcagaag gtctaaaagt
     1681 tcgtccacgc cggtgccctc ggttgaggag acaagggcgc agggaatggc gccgccgtaa
     1741 ccttccacaa aaacgtcatt ttcggctaag ctttgtttgg ttcggtccgg gttagcttca
     1801 ggtttgtcta ttttgctgat agccaccacg aaaggcttgt tgtgttcttt gattgatttg
     1861 agcgcttcca gggtttgtgt cttgacgcct tcttcggctg agacaatcaa gacggcaata
     1921 tcgcaaattt gcgcaccccg aacacgcatt gcctcaaaag cggcatgacc gggggtgtcc
     1981 aaaaaagtta ttttttgatc tttatgaaca acctcataag cgctcacgtg ttgggtaata
     2041 ccgcccgact ctctttctac gacattggtt tgtcggatgt aatcaagtaa cttggattta
     2101 ccgtggtcaa tatggcccat aacggcaata accggcgggc gagctatttg tttgtttttg
     2161 tcagtttcgg ccatattttg tattaaacca ataaaatagg ccgaagacaa gcctgaatga
     2221 ctggggcgat taaaaatcgg aagccttgtg aacgtccgat ttgtctcgga atttagctcg
     2281 aagtttgttg ctcggcgcgt tcaatcgccg cgcgttcttc ttgggttagc tcatactcgg
     2341 atttgccgtc ggaaatcggt ttggcgtaga tactgacttt gtcgcgcgag taaaggctgg
     2401 aaatgaccac cccatcacct tgctcgttaa ggaagctagt ggcaaagctt tggttgccgc
     2461 cttggtcctt gaagggatta aagcgaacgg tgtgaacttt ttgaattgct ttggctaggc
     2521 gtttgttgat gttgtctatc tcttgagcaa tgagcaagat tcgcttgtcc attttttgga
     2581 tggtgaccac attgccggca atggtgtctt ccagatcgcg ggcatttttg ccggaaagca
     2641 aactcttcag tcttttttcc aaaataacca ttctaaccaa aacaaaaatc acgatggcga
     2701 ttaggcccaa agtgacgaca agcaaagtat ttatatccat atcttaattt taaacaaatt
     2761 atgataaaat gccaactatg gaactaccgt ttaaaagtgg aaaaactagg cattattggg
     2821 atacggcagc cgcgacgcct ttggcgccga tagtcgaaag ggcgatggtc aaaactgcca
     2881 aagactttgg caaccccgcc agcattcatc gagagggagt gttagcgcag gaaaagctgg
     2941 cggctgcgcg ccaaaccgtt gccagattga ttgccgccga accggatgag attgttttta
     3001 cgggaggcgg aacggaaggc gacaacctgg ctattttggg cgtggcgcgc gcgcgtagtc
     3061 aagtcggtca tcttattacg accaccgtcg aacacaaagc ggttcttgag ccttgccgat
     3121 atttggcgga ccttggccac cagatttctt atttgccggt ttccgctgat gggttggtta
     3181 atctcaaaga agtggaaaaa acactcacga cggaaacttt tttggtttct ataatttacg
     3241 ccaacaatga aattggaacg atccagccga taaaagaaat tgctaaggtt gtcaggcgtt
     3301 ggcggaaaga acacaaaacc aatttgccgt atttgcatat tgacgcttgt caggcgccgc
     3361 gttttttaaa tttgaatgtt ttgcagttgg gagtggacct aatgacggtt aatggctcta
     3421 aaatttatgg tccgaaaggg gttggttttc tttatgtgaa gcgaggggtt cgacttctcc
     3481 cgtttttgct tgggggtggt caagaagaag gtcgccgctc tgggacgcat aatttgccgg
     3541 cgattgttgg tttggccaag gcgctagaaa tttgtgaagc cgaaaaagaa gcagagacag
     3601 aaaaattgac ggcgattcgg gattatttgg ccacccaact gacgaagatt gccggtctaa
     3661 aattgaatgg cggttttggc aagaatgaaa gattgcccaa caacttaaac ttttcggttg
     3721 agggtttgga gggcgaacaa ctagttttgg aattggacgc tcgcggcttt gcagtttcgt
     3781 cgggtagcgc ttgctcattt ggtgctgatg acggctcgca tgtcgtggca gccctcggtt
     3841 atggcgaagc gcgggccttg ggcgctgttc gagtaagttt gcctcgcgag acaaaactgg
     3901 ctgaggctaa aaagtttgtc gaaaaagtga aagaaatttt aaaaaaatac aaggggtgat
     3961 tttatttagc tgaacttttc ctccctttct tttttatgct ataattttct tatgaatata
     4021 aatgatttga ataaaagtca gttttttctt ttaattttgt tagtgatgtt tgtaacttcg
     4081 gttacaacgg cgattgtgac ggttactttg cttgataagt ctccaagtgg cgggttttcc
     4141 ggcgcggtaa cgcaagtggt tgaaaaaaca attgaaaaaa ttgttcctgg cgcaacgacg
     4201 acgattgtga aaattatccg tgaggcccct ttgcccaatg aaggcgaatt gattgcccag
     4261 gcggtaaaca aagtttcgcc cagcgtcgtg ggagtgtcgc agaaggataa agacagtaac
     4321 aaaaaactgg gagcaggttt tgtcttgtca aacgatttag tcgtgacggg cgccaaaatt
     4381 ttgccaccgg atataaagga attgtctgtt tcttttggta aggcgaatct tcaggcagat
     4441 ttaataagcc gagacgataa tcatggggtc gcttttttcc gcattaatgc ttccggtacg
     4501 gagattgttc aaaccggaca gttggcggaa gaaggcccca cggttggcca aacggtggtg
     4561 ggtttaagcg tcgccagtga cggttcggcc gagattgcga caggtatcgt gattgatttt
     4621 gtcagcgcgg cagcaactag cacgaaccag acgggaacca gcacggccgg tttgattagg
     4681 accaatgccg taaattccga taatgtcggc ggcccgataa ttgacaaaca agggaaaatt
     4741 gtcggcattg gcattgctcg cggttatgcc ttggcggcgg atagcctaaa aaatcttatt
     4801 gaccaaataa aataaccgtg ttaagataaa agagtgtttg agaagtctga aagctgatga
     4861 gcctgaatgt ctgaagcgga tattaaatta taaattaatt ttagccgttg aagttccgga
     4921 gagaaatttt gtctctttgt ttggctaggc caatggttct cgggtttagt aaattacttg
     4981 tataaattta ttgactatta atttttaaac tttatgcctc cattccaaaa ctttacttca
     5041 aaggcccgtg aggccatccg caaatcacac gaactggcga ttgaacgcgg tcaaaatcaa
     5101 gtcaatccaa tacatatgtt ggcggcttta gttttgcagg atgaaagttt ggttgtttct
     5161 atcatcgaaa agttggaagt ggactcaatc gctttgactg attatatctt ggatagtatt
     5221 gagggcccgg gcatgggcag tgttgtcagt ccgaattacc aaatctatct aacaccggaa
     5281 ttggtgcgag tgtttgaaga gtccatcaag caagctggta gcctgcgtga tgaatttgtt
     5341 tccaccgaac atcttttgtt ggcaattttg gatattccga gtcaagctaa agaaattctt
     5401 aatcgcttca agattacgcg cgataatgta tttcggattt tagaagaatt aaaatccggg
     5461 aaaatgccaa acttaactga aaccaaaaaa ttcaaagcca ttgaaaaatt caccagaaac
     5521 ctgactgaaa tggcgcgcgc ggataagtta gatccggtta ttggtcgtga cggagagatt
     5581 cgtcgcatta tggaaatttt gtcccgtcgc accaagaaca atccggtttt ggttggtgaa
     5641 gcgggaaccg gaaaaaccgc gattgtggaa ggtttggcga ttcgcattgc taaaggggac
     5701 gcgccggaat ctttgcgcgg caaagaaatt gtctcactgg atttgggtct gcttttggcc
     5761 ggaactaaat accgaggcga gtttgaagaa cgtttaaaga gtatcatcaa agaagtggaa
     5821 aaatcggaaa ataaaattat tttattcata gacgaaattc atacgattgt tggcgccggc
     5881 gcggccgaag gcgcgattga tgcttcaaat atgttaaagc cggccttggc gcgtggcgat
     5941 ttgcggacaa ttggcgcgac gacaatcaaa gaataccaaa agtatattga acgcgaccag
     6001 gctttgacta gacgtttcca gccggtattg gttgaggaac caacagttga agacgcaatt
     6061 actattttgc gtggcctaaa agacaaatat gaacttcacc atggtgttca tattactgat
     6121 gattccatca tctcggcggt caacttgtcg gcgcgctata ttactgaccg ttttttgcca
     6181 gataaggcta ttgacctgat tgacgaagcg gcgtctgctt tgcgtttgca gttggaaaat
     6241 aaaccggcgg ttttggaaga caatgagcgg aaaattatgc gcttggaggt ggaaaaagaa
     6301 gccttgaaaa aagaagtatc gttgaatgac aagaacaaaa acgccaaaac tcgaatcaaa
     6361 gaaattgaca cggaaattgc cgaattgaaa gaaaaagtaa gtgagctgga attgcgatgg
     6421 aaaaacgaaa aacaattgat aaatgaaatt cgcgatatca aagaagaatt agaaaaattg
     6481 aagtttgaag ccagtcaggc cgaactgcgg gccgacttgg gtaaagcggc ggaaattctt
     6541 tatggtcgag taccggaact gcaaaagaat ctgaaaatgc gcgagaacaa actgaaaaaa
     6601 ttgcaggcga cacgcaaagt gttgaaagaa gaagtggccg aagaagaagt ggccaaagta
     6661 gtgtcacgct ggactggtat tccggtcacc aagatgttag aagaagaaag cgaaaaactg
     6721 aatcgaatgg aggaggagct tagaaagcga gttaagggtc aagatgaggc cgtcgaaaaa
     6781 gtgtctgaat cagttcgtcg gtcgcgcgct ggtatttcag accccgaccg gccgattggc
     6841 tcgttcatct tcttgggtcc gtcaggtgtt ggcaaaacgg agctggctcg cggtttggct
     6901 gagtttttgt ttaacgacga aaaggctttg attcgggtag acatgtccga atatatggag
     6961 aagtattctg tttccaaact gattggttcg ccgccgggtt atgttggaca cgaagaaggc
     7021 ggcaatttga ctgagcttgt tcgtcaccgc ccctattcca tcttgctttt tgatgaagtg
     7081 gaaaaagcgc acccggaagt ttttaatatt ttacttcagg tgcttgataa cggccgcctg
     7141 accgattcca aggggcgagt agtaaacttc aaaaatacca tcattattat gacttccaat
     7201 attggcagtc agtatattaa gaactttaat aaaattggtt tttcagggcg cgataaggcg
     7261 gaggccggtt ttgaagatgt caaagaaaaa atgatggata atctaaaaga ccacttcaga
     7321 cccgaattct tgaatcgctt agatgaaata atcatcttta atccgttgtc tgaagaagtg
     7381 ctaaaacaga ttgtggacat ccagttgggt ttggtcaaag aacgtttgat gaaaaaaggc
     7441 gttttcttgg atgtctcaga agaggcaatg ttttatttgg ccaaagaagg ttttaaccca
     7501 gagtatggcg ccagaccgct taagcgcttg attcagagca aaatcttgaa tccggtagcc
     7561 aagtttatca ttgctcgttc ggtggacaat gggggaacag taacagtgga gatgaaagag
     7621 ggcgagccag cagtggaatt aaaaaaggtt tttcgccggc gcgctagtaa cgttaggggc
     7681 gattccaaaa cttcagctag caaaaagtag gtttgcgata tttggtgtta tatattatga
     7741 taattaggcc tcaaaagaga cctaattgcg taggtggcgg agtggtcaat cgcacctgac
     7801 tgtaaatcag gcggcttcgg cctacggggg ttcgaatccc tccctacgca caaaacaatc
     7861 ggctttatgc cggttttttt gtgagtagga gcaagtgaat tgattcactt gcgtgagggg
     7921 ttcgaacggc ggagcatgtt tcgctcagta gagcgaaacg cgagccggtg cctagcccga
     7981 gccgagtgac tgacgaggcg agaggcgaag gagaatccct ccctacgcac aaaacaatcg
     8041 gctttatgcc ggttttttgt atgaacgtct tttttggttt tttattttgc agctagggaa
     8101 aaaagatggg atattggtgg gtgaagaaaa aacatttgg
//
LOCUS       DAOOLO010000027         8004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_10596_length_8004_cov_5.747641, whole genome shotgun sequence.
ACCESSION   DAOOLO010000027 DAOOLO010000000
VERSION     DAOOLO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10596_length_8004_cov_5.747641"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLH96_02290"
     CDS             <1..259
                     /locus_tag="PLH96_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB60624.1"
                     /translation="IRENLHRHPTVKFEEVVGVSLRETYIRSINTTVVIIIVLVSLLI
                     WGPASTYYFALILLIGMVAGTYSSIFIASNLLISWEKFGQR"
     gene            645..2481
                     /locus_tag="PLH96_02295"
     rRNA            645..2481
                     /locus_tag="PLH96_02295"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            4209..4282
                     /locus_tag="PLH96_02300"
     tRNA            4209..4282
                     /locus_tag="PLH96_02300"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4243..4245,aa:Ile,seq:gat)
     gene            4499..5875
                     /gene="murD"
                     /locus_tag="PLH96_02305"
     CDS             4499..5875
                     /gene="murD"
                     /locus_tag="PLH96_02305"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPB60625.1"
                     /translation="MTYKDFFKNKKITVMGLGLLGRGIGVVKFLAKNGADLIVTDLKT
                     KEQLSSSLKKLAVFKNIKYVLGEHRLEDFRNRDMVIKAAGVPLDSPFIAEAKKNKIPI
                     EMDASLFAKLAPTGVKIIGVTGTRGKSMVTHLLYEILKENFGSSKVHLGGNVRGIATL
                     PLLKKVKPGDFVVMELDSWQLQGFGESRLSPPVSLFTTFLSDHQNYYKNSMEQYFADK
                     ANIYRWQTKDGILLARPQAVKIIKEKDKAGIKGRFKVVKKSDLPANWKHNLLGEHNLE
                     NIALALSAARELGALDKVSKKAIAAFPGLPGRLEFIKEKGGIKYYNDTNATTPDAVLV
                     ALAALKKYKGKIILLGGGADKNLDYATYAKAVRQNVKALALFRGTATDKILEVLWSRS
                     VLGRSRTDLGQNKVEVFDNIREAFAWAKAQAKRGDIILLSPGAASFGVFKNEYDRGDQ
                     FNKLVKRI"
     gene            5924..6406
                     /locus_tag="PLH96_02310"
     CDS             5924..6406
                     /locus_tag="PLH96_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60626.1"
                     /translation="MKETEEKKTIEPLTLKSLLALIEASAGTTMFRHLYAEVDGVEQD
                     ILEDGNLSCAFYVSGVLAIFGLIDRLHTTVSGTVTALEKAGWQKTDNLKPGCVIVWDK
                     PRDNSHNHQHIGFYLGDETAIANSTEKKVIAKNHYTYGGEEQSSQRQILAIYNHPSLS
                     "
     gene            6470..7822
                     /gene="murC"
                     /locus_tag="PLH96_02315"
     CDS             6470..7822
                     /gene="murC"
                     /locus_tag="PLH96_02315"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPB60627.1"
                     /translation="MSIISNRQKIHFIGIGGIGMSALARMFLGEGKQVSGSDRAPSLV
                     TEGLEKLGAKIFYEQEAENIAADVDLIVYTIAIPADNSELLAARAKGIDCLTYPQALG
                     LISAEKYTIAVAGTHGKTTTTAMIAEMMIAASLSPTVVVGSLLKQKNTESSFPGGTNF
                     IAGDSKYLVVEACEYQRSFLNLSPQIAIITNIDNDHLDYYKDFADIQSAFAEFVSKVP
                     EDGFVVCDKKDPRLELVLGQTKATVVDYKNFLEKISSSGLTVPGHHNLLNASAALAVG
                     EILQISEGTSLKALLGFSGVWRRFELKGEMETGALVYDDYAHHPTEIEAALAGAREFM
                     SNRQMTGKLFAVFQPHLYSRTKILKEDFATKLALADEIIMAPIYAAREPADPTISSEI
                     LAEAIARKNRNVRVLRDFAEIAEALRTDTEEGDLIMTIGAGDIYKVGEGLTRNVKIKV
                     "
BASE COUNT         2206 a         1585 c         2077 g         2136 t
ORIGIN      
        1 gattcgagaa aatcttcatc gccacccaac ggttaaattt gaggaagtgg tgggtgttag
       61 tttgcgcgaa acttatattc gctccatcaa cacaacagtt gtcattatta ttgtcttggt
      121 gtccttgctc atttggggtc cggcctccac ttactatttt gcgttaattc ttttgattgg
      181 gatggtggct ggtacctatt cctctatctt tattgcttca aacctcctga ttagttggga
      241 aaaatttggt cagcgctaaa ggcgttgtcc ggaggcagat aaaacgcagg gttatacaaa
      301 ccgaacgcga gttttattaa gcgttgggtt ttaggttgta agccggcaag agagaagata
      361 aaattggctc attttttcat ctttttcaac caccaaccct caatttctaa cccctgccgg
      421 gaggtgttga ctttctattt ttaccatata taataagatt taaggctact caaggtagtc
      481 gcttgttcag ttcattgtca atttaatagt tacaaagcat tacaagtccg cctatgttct
      541 tatggaacta aggcgagaca aagtcagcaa aatacttttc tcggcatcag taaccgagga
      601 aaagccaaat ctggaagaat attatttttt cttctgttcc gttttttatt tagagtttga
      661 tcttggctca gggtgaacgc tggcggcgtg gataaggcat gcaagtcgaa cggacgtacc
      721 tcactgtgtt cgggattaag aaattaaaaa ttgaaaatgc aaattttaaa attatggagt
      781 ttgtaaagcg gactcaatga tttaatattt ttatttttta tttttaaatt ttgttaaccg
      841 aacttggcga ggtgcgtccg tggcgaacga ggtagtaata cgcaggaatc tactcttaag
      901 tcggggataa cccgtcgaaa ggcggggtaa tactcgatgg catcgtgctt cactattgta
      961 aatcacttac aaaaagtttc agtggtttgc ggtagcgagg cacgattaaa gatttatcgc
     1021 ttaagaaaga gcctgtggga catcagctag ttggtagggt aatggcctac caaggcaatg
     1081 acgtctaggg gaggtgagag cctggccccc accgatggga ctgagacacg gcccatacac
     1141 ctacgggtgg ctgcagtcga gaatcttccg caatgggcga aagcctgacg gagcgacgcc
     1201 gcgtgatgga tgaagttctt aggagcgtaa acatctttta tgagggaaga agtggttgtg
     1261 cctcacagag tttggcacaa gcgcaaaact taaaacgcaa aacgtaaagc ttaagcaaaa
     1321 aaaataaaaa cttaagaaaa atcagtctga tttttccagt ttgaaacttt tgagtttaag
     1381 ttttgagttt tgaactttga gttttgagtt tgttgagccg agctttgcga ggcgcaacta
     1441 tatgacggta cctcatgaat aaggggctgc aaaactcgtg ccagcagcag cggtaaaacg
     1501 agtgccccaa gcgttacccg gaattactgg gcgtaaagag tgcgtcggtg gttctgatta
     1561 gtcctccgtt aaaactcccg gcccaaccgg gaagctgcgg aggaaacggc agaactagag
     1621 aaggcgaggg gtctatggaa ctcatggtgt aggggtgaaa tccgttgata tcatggggaa
     1681 caccaaaagc gaaggcaata gactggcgcc tgtctgacac tcaagcacga aagcgtgggg
     1741 agcgaacggg attagatacc ccggtagtcc acgccctaaa cgatgttctc tagctttttg
     1801 gagtatcgac cctctgagag gcacagctaa cgcgttaaga gaaccgcctg ggaagtacgg
     1861 ccgcaaggct aaaactcaaa agaatagacg ggggctcgca caagcggtgg attatgtggt
     1921 ttaattcgat gctaaacgaa gaacctcacc aaggctagac atccaactga agggtctggg
     1981 aaaccaggtc tctcccacaa ggacagttgg acaggtgatg catggttgtc gtcagttcgt
     2041 ggtttgaatt gttcccttaa gtggggtaac gaacgcaacc cttgtcgtct gttataagtg
     2101 tcagacgaga ctgtcctgct cagcagggag gaagcgagga tgacgccaaa tcagcatgtc
     2161 cctcgacgcc ttgggctaca cacataatac aatggtcatt acagagagtc gcaatatcgt
     2221 aagatggagc taatctttaa aagtggcccc agttcggatt gaggtctgca actcgacctc
     2281 atgaagccgg aatcgctagt aatcgcaggt cagctatact gcggtgaata cgttctcgag
     2341 ccttgtactc actgcccgtc aagccagggg agccgggagt gcccaaagtg ccgtttcggc
     2401 ggtcctaagg caagctcggt aacaagggtt aagtcgtaac aaggcacggc tagcggaagc
     2461 tggtcgtgga tttatttcaa attgaactgg tcaataatag tagacattga aagttgaaaa
     2521 ttggaagttg gaaagaggtt tatctttctt ccgcttccta acaaactttt catagcacta
     2581 ttatcctgac tggtcgatca ccacgccaat ctgattggcg tggtgagagt gatggttctc
     2641 cgcactcttt cggttttttt cgaagaaatc gaaataacaa ggaactcgcg ctcgtcggcg
     2701 cgtaaccggc gaaacggaac agaagaaaga ataatattgg ctctgacttt ttaatgcaaa
     2761 aacaaacacc ccgtgatctt tggtcgcggg gtgttgtctt tttctaaaaa gagttttaaa
     2821 ctaaggaaga attgagttct tgaggacagc taaaatgaat gcttgcgcac aacttgcgcg
     2881 caaaaagact ggaggcgtaa aaaatgtcag aaaaaatttg tcttaactgc gggggaccag
     2941 tattagaaaa cttgcctcag gttgagatgg ggaatattag ggggtacctt tgtggcgaat
     3001 cggggaatcg ttgctggcag cgtcttgggg aagaagcttt tctgacggta ggccacgatt
     3061 ctgaagaagg ctcaagggag aagcaatgct attttgaagg tatgcaagtt tagttttagt
     3121 ttgtccacag ccgccgagat cccctctggc ggttttcatt ttcttcatca aaaaacttgc
     3181 agggtaggtt caaaagaatt actatcaaac cagagagatt ctcgccaatt tgagctggcg
     3241 ccaaaaggag aaaaaaatgg acaacttgaa gtgttttgtc tgtggaaggc tcctggctgg
     3301 cggagacctg ggagcaatta tttggtcggg ggaatacaag gtcgtttgcc aaggccatgt
     3361 tcctgaagga gcgagtgtgg tggtggaaaa ttggccggcc gccgggctga tttacaagtt
     3421 aaatgagata aggattggtt aggtcggcag aaaaccacga ttaaccgtcg ggagtatata
     3481 aacttcgggc ggcttttatc tttgagttaa gtcggtggcg aaccttaaaa agacccctaa
     3541 actggggctt tttgggtaaa aaatgaagaa atgattgact tttaatatta aatatgctat
     3601 aatgatagat agagcatgtg ttgtttggaa aaccgccggg gagcggcgga aggaggagaa
     3661 aatggggaag ttattggctc aaggacttgg ggtggtcctc ctcggggcat gtctcgggct
     3721 ttgttggttc ttcgccagcc atgagttggc gaagcccgtc gggttttatt tcggctccga
     3781 attactcgga gttggagtga aagagcccag tcggggattc gtccagtata gactggacga
     3841 gttcttgccg atttacggcc aggtgcccga gttgcaccag gaggtgcaag tagggccaaa
     3901 ttggcaaccg gcgccgcggc ccggggaaga cccggagcct ggctcggggg gcagccccga
     3961 caaagagtag ccatagaccc gtcacggatt agccggacgg gtctttcttt ttgacttgtt
     4021 tttggcgttt ttttttgata tatttctaga gaaagttggg gtttaaattt ttaggtttta
     4081 aacttttaaa ttttaactta acacaacgcg cgtgtctctc ccttcttcgc gcaagtcaga
     4141 acctgagctt tggatgggca agcaggtgga atgatatctt gaaaattaaa taatctggtt
     4201 ggcaacgtgc gcgtgtagct caggtggtta gagcgcgtca ctgataatga cgaggtcgta
     4261 ggttcgagtc ctaccatgcg cacgttgctc gtcaattgat ttaattttca agatactatt
     4321 tcatagagcg atgaaagata tcggtacgcg ttttcatctg ataatgacga gtcctaccca
     4381 aggcggaccg agccttgtta ggcgcgcaaa taatcccgcc gaagcgggat tatttgcgcg
     4441 cctgagtatt ttaaattaga gctttcatct gttttaagtc ttatgctaga atgtttttat
     4501 gacttataaa gattttttca aaaataaaaa aatcaccgtc atgggactgg gtcttttggg
     4561 gcgggggatt ggcgtggtca agtttttggc gaaaaacggc gcggatttga ttgtgactga
     4621 tttgaagaca aaagagcaat tgtcttcttc tttaaaaaag ctggctgttt tcaaaaatat
     4681 caaatatgtc ctgggagaac atcgtctgga agattttcgc aatcgggaca tggttataaa
     4741 ggccgccggg gtgccgcttg attcaccgtt tatcgctgaa gcgaaaaaaa acaaaattcc
     4801 gatagaaatg gacgcttcgc tttttgccaa actggcgccg actggcgtaa aaataattgg
     4861 ggtgaccggg actaggggca agagcatggt gactcactta ttgtatgaaa tcttaaaaga
     4921 aaattttggg tcatcaaagg tccatttggg cggtaatgtt agggggattg cgactttacc
     4981 cttgttgaaa aaagttaaac caggggattt tgttgtgatg gaacttgatt cctggcaatt
     5041 gcaaggtttc ggcgaaagcc gccttagccc gccggtttct ttgtttacaa cttttctgag
     5101 tgaccaccaa aattattaca agaatagtat ggaacaatac tttgccgaca aagcgaacat
     5161 ttatcgttgg caaacgaaag atggtatttt gttggcgcga ccacaagcgg tcaagataat
     5221 aaaagagaaa gacaaggctg gcatcaaggg gcgatttaag gttgtcaaaa aaagtgattt
     5281 gccagccaac tggaaacaca acttattggg tgagcataat ttggaaaaca ttgctctggc
     5341 cctttcggcg gcgcgtgagt tgggggcgct tgataaggtt agcaaaaaag ccattgccgc
     5401 tttcccgggc ttgccggggc ggttagagtt tattaaggag aaaggcggaa ttaaatatta
     5461 caacgacacc aacgctacca ccccggacgc ggttttggta gccttggccg cgctcaaaaa
     5521 atataaaggc aaaataattt tactgggcgg cggggctgat aaaaatcttg attacgcaac
     5581 ttacgctaag gcggtcaggc agaacgtcaa ggccctggcg cttttccgtg gcaccgcgac
     5641 tgacaaaatt ctagaggttc tttggtctag gtccgtccta ggccggtcta ggacggacct
     5701 aggccaaaat aaggtggagg tctttgataa cataagagag gcttttgctt gggctaaagc
     5761 tcaagccaaa cgtggggata ttattttact atcaccaggc gcggctagtt ttggagtgtt
     5821 taaaaatgaa tatgatcgag gcgaccaatt taataagtta gtgaaaagga tataatttat
     5881 tgtataagga agatagagtt tgttatatta gacttagaac agcatgaaag agacagagga
     5941 aaagaaaaca attgaaccat taaccttaaa atctctgttg gcgctcatag aagccagcgc
     6001 gggaacgaca atgtttcgtc atctctatgc cgaagtggat ggggttgaac aagatatttt
     6061 agaagacggc aacttatctt gcgcctttta cgtatctggc gttttggcga tttttgggtt
     6121 gattgaccgg ctgcacacga cggttagcgg cacagttacg gcgttagaaa aagccggctg
     6181 gcaaaaaacg gataatctaa aaccgggttg tgttatcgtc tgggacaaac cgcgcgataa
     6241 tagtcacaat caccagcaca ttggttttta tttgggtgac gaaaccgcaa tagccaacag
     6301 taccgagaaa aaagttattg ccaaaaacca ctacacatac ggcggcgaag aacaatcgtc
     6361 tcagagacaa attttggcaa tctataacca tcccagctta agttaaaacc aacacaggag
     6421 gtcaaaaagt caactacaaa atcgctgttt tttgccgtat aatatctgaa tgtcaataat
     6481 ttcaaacagg caaaaaattc attttatcgg aatcggtggt attgggatgt cggcgttggc
     6541 gcgaatgttt ttaggcgaag gcaagcaagt gtccggctct gatcgcgcac cgtctttggt
     6601 caccgaaggg ttggaaaagt tgggggctaa gattttttat gaacaagaag cggagaatat
     6661 cgccgctgat gttgatttga ttgtttatac gattgctatc ccggctgaca actccgaact
     6721 cttggcggcg cgcgccaagg gcattgactg tctcacttat ccgcaagcac tcggtctgat
     6781 ctcggccgaa aaatacacga ttgccgtggc cggaactcac ggcaaaacaa caaccacagc
     6841 gatgattgcc gaaatgatga tagccgctag tttgtcgccg acagttgtgg tcggcagctt
     6901 gcttaaacaa aaaaacacgg aaagttcttt tcctggcggc accaatttta ttgccggtga
     6961 tagcaaatac ctagtcgtgg aagcttgcga gtatcaacgt tcttttttaa atttgtcacc
     7021 ccagattgcg attatcacca atattgataa tgaccacttg gattactata aagacttcgc
     7081 cgatattcaa tcagcttttg ccgaatttgt atcaaaagtg ccggaggatg gttttgttgt
     7141 ttgtgacaaa aaagacccgc gcctggagtt ggtcttagga cagaccaagg cgacagttgt
     7201 agattataaa aattttttgg agaaaatatc atcatccggg ctcacggttc ctggtcatca
     7261 caacctctta aacgccagcg cggctttggc ggtaggcgaa attttgcaaa tttctgaggg
     7321 aacctccctt aaggcgttgc tcggttttag cggcgtttgg cggcggtttg aacttaaggg
     7381 cgaaatggag accggcgctt tggtatatga tgattacgcg caccacccaa ccgagattga
     7441 agcggctctt gccggcgcgc gggagtttat gtctaatcgc caaatgaccg gcaagctatt
     7501 cgcggttttc caaccgcatc tctatagtcg caccaaaatt ctgaaagaag actttgccac
     7561 caagctagct ttggcggacg aaattattat ggcgccgatt tacgcggcgc gcgaaccggc
     7621 cgacccgact atcagttccg aaattttagc ggaggcgatc gcccgcaaaa atcgaaatgt
     7681 acgcgtcctg agggattttg ctgaaatcgc ggaggctcta aggacagata ccgaagaagg
     7741 ggacttaatt atgacaattg gcgcagggga catttataaa gtcggggaag gcttaacgag
     7801 aaatgtaaaa attaaagttt gagctttaat ttttacattt cttatgctat ccttttctca
     7861 tatgagacac attatttggc gtaacagagc cagcgcgatt ctgggtttac taatcatgat
     7921 tattccgttt accggtttcc cggaaccttt gcgcacggct cttattgttt tgtttggttt
     7981 gttgattgtt atttttggtt ttgc
//
LOCUS       DAOOLO010000028         7103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_12526_length_7103_cov_6.165437, whole genome shotgun sequence.
ACCESSION   DAOOLO010000028 DAOOLO010000000
VERSION     DAOOLO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12526_length_7103_cov_6.165437"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLH96_02320"
     CDS             complement(<1..335)
                     /locus_tag="PLH96_02320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB60628.1"
                     /translation="MKKTVKSLTDLESLAGELLKKLKKDQQATIVTLAGDLGSGKTAF
                     TQMIGKKLGIKTKITSPTFVIQKKYELPLSGPQGISTGYKQLIHIDAYRLNCGRPLKQ
                     NQIIRISHN"
     gene            1266..2978
                     /locus_tag="PLH96_02325"
     CDS             1266..2978
                     /locus_tag="PLH96_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HPB60629.1"
                     /translation="MIDPNSQTTDKHLQDLREKEAEELAQTLSGRYGLPYLDLSKTAI
                     NTDALRLIPEDEARLAAVAAFKVVNKTLSLAILAPNKPEAQAVIKDLEGKNFKVELFM
                     VSEVGLESAWARYSEVSLSEKTKAGLIEISNEDVTRLTEQFKTLTDIKTEIDKESGLA
                     LSQGGISGILEVILAGGLVTDASDIHIEPEQETIRLRYRLDGVLQDVADFPLKIHRQI
                     ISRLKLVSGMKLNVKEAAQDGRFSIALENSDIEIRSSVIPSAYGESVVMRILNPKTIE
                     LTFAKLGVETKLFEIFNREIHKPNGLVLLTGPTGSGKTTTLYAFLREINSPENKVITI
                     EDPIEYHLGGINQTQVNKKQGYTFLLGLRSALRQDPDVIMVGEIRDSETAKTAVQSSL
                     TGHLVFSTLHTNNAQGAIPRLIDLEVNPKIITSSLTLSIAQRLLRKLCPHCKKEIAPT
                     ESEKELLSAVIQSIKEKRSELVPENWQGEITEANKIFVAGGCEQCNGIGYKGREGVFE
                     AIVMDDAVAAATITNPSEKEIKIAAKPQGILDMRQDGILKVITGRTTLEELSRVVDLY
                     EEII"
     gene            complement(2971..>3199)
                     /locus_tag="PLH96_02330"
     CDS             complement(2971..>3199)
                     /locus_tag="PLH96_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60630.1"
                     /translation="RFFFVFLSSLQTSKQSRPVYRPNGFFQEDASVFTLAVSFTEAIL
                     EELSNFRCTSKGIAWRFVVLIRFSVCKERFK"
     assembly_gap    3200..3299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3300..4163
                     /gene="ruvB"
                     /locus_tag="PLH96_02335"
     CDS             <3300..4163
                     /gene="ruvB"
                     /locus_tag="PLH96_02335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPB60631.1"
                     /translation="PEHLLFYGPPGLGKTTLAYLVAKEMNAQIKVTSGPAIEKVGDLA
                     SILTNLSAGDILFIDEVHRLNKMIEEVLYPAMESGHLNIIIGKGPGARVIQIDLPPFT
                     LLAATTRIAAISSPLRSRFSGGTFKLEFYTNEEISEIIKRSAQILGIELGDESAFSIA
                     KRSRFTPRIANHLLKRCRDYAQVTKKAISPKTTNEALSLMGIDELGLNEPDRKIIEVI
                     IDKFGGGPVGVGTIATALSEEEATIEDVYEPYLIQLGFLERTPRGRVVTDKGYEHLGF
                     DLPPKNQPKLI"
     gene            4226..4765
                     /locus_tag="PLH96_02340"
     CDS             4226..4765
                     /locus_tag="PLH96_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB60632.1"
                     /translation="MSGHNKWTQIKHKKGAEDIKRSKLFSMLVRTINVEARLANGNRE
                     APGLKAAIVRAKAANMPLDNIERAIEKGSGAGAESFEEVVYEAYGPGGVALLIVGLTD
                     NKNRTTPEIKHLLSKHGISLSPQGATQWAFIKKEDEWQAQNTLPLAKTEQEKLQALLE
                     ALGEHEDIKNIFHNADEIA"
     gene            4746..5243
                     /gene="ruvC"
                     /locus_tag="PLH96_02345"
     CDS             4746..5243
                     /gene="ruvC"
                     /locus_tag="PLH96_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPB60633.1"
                     /translation="MPTKSLKILGIDPGYDRLGLAVIEKSQTGLKLLYSTCLQTNKKD
                     SLSSRFLLLGRELKKIIKKEKPDVVALETLYFSVNQKTASKVAEVRGMIAYICAESGL
                     PISEYNPMQVKSCVAGWGGANKKQIIALVKKQIVVPAKVKIDDEYDAIAIALTHSVMA
                     KMLSL"
     gene            5427..5585
                     /locus_tag="PLH96_02350"
     CDS             5427..5585
                     /locus_tag="PLH96_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60634.1"
                     /translation="MDELYNDDVLGGVALSDDDSFGDDEELENLDDEVETDDEDDSFG
                     GSDDDDEE"
     gene            complement(5658..6872)
                     /gene="tyrS"
                     /locus_tag="PLH96_02355"
     CDS             complement(5658..6872)
                     /gene="tyrS"
                     /locus_tag="PLH96_02355"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB60635.1"
                     /translation="MNLKEKEERIQEVLTRSISKIYPTSEKFAELLHSNRPLRIYMGI
                     DPTATYVHLGHATNYLLLRRLHQLGHKIIVLVGDFTAMIGDPSDKKAARQRLTKNEVK
                     KNLKDFKKQIGKILDFKDKKNPVEFVLNSKWLSKLNFNDLVELASNFTVQQMLERDNF
                     EKRLKEQKPLYVHEFFYPLMQGYDSIALDADIEIGGTDQTFNMLAGRTLLQAYKQKEK
                     IVVTTTLLENPVTGEKLMSKSLGTGIGLDESPASMFGKTMALPDEAIIQCFVDCTDLP
                     LAEIENIKKELHAGANPRDLKMRLAWEIVKLYHGDKEADKAQNFFVTAFQKKEIPADI
                     KIINAVKGTLLMDILLKNNLVKSKSDFRRLVAGGGLSEINGEKITEPTAKIEKDVTLK
                     IGKKNFLKIELK"
BASE COUNT         2010 a         1653 c         1479 g         1861 t
ORIGIN      
        1 aaattatggc taatccggat aatttgattt tgcttgagtg gccgaccaca atttaggcgg
       61 tatgcatcaa tgtggataag ttgtttataa ccagttgaaa tcccttgcgg tccagacagt
      121 ggtaattcat actttttctg aatcacgaag gttggactgg taattttggt ctttattccc
      181 aattttttac caatcatttg cgtaaaagcg gttttgccgg aacccaaatc acccgccaag
      241 gttacaatgg tcgcctgttg gtcttttttt agctttttta gaagctcgcc ggccaaactc
      301 tccagatctg tcaaactttt tactgttttt ttcatagcgt cattttataa gctggccaaa
      361 gaaaaagcaa aaacagaaca ccaagaaaaa ctgagaaaag aaaaaaggcg cgagagcttc
      421 gctctcgcgc cttgactgtt gcgctaccgg tagatcggca ccggcacgcc aaccggccga
      481 ggtggacgcc agccgtagtg gccatgagtc cccccgtagt accccccgga ccggtaaatt
      541 ggtaccggta ccccgactgg tcggggcggg cgccagccgt agtggccgta tccgccaggg
      601 cggtgatgac caccccgacc gccggtcaca accgtcacca cctgtggcaa aaccacagat
      661 ggcagcgacc tggtcgcggg ggctggctga gccagccgga ccgtaccccc gcaatagggg
      721 caaagcgccg cgccattggc cggcaccgtg atcccctgtc cgcactgcgg acaggctccg
      781 ctgaccggtc cgaccgagac cagctgcgac cccgccggcc ccatcgtggt ggcgcaaccg
      841 ccaacaccaa cggccaggac caccgacaac acaacaaaaa actgcttcgc gtgtttcatc
      901 tcgacctcct tccaccaagg gcggatcccg tctttttctt catttcaacc ctctacctat
      961 cattataaca aataatagcg aaaaagtcaa gctaaacagc tccccgggtt tgcaccccat
     1021 ttatggccca aatcaccctt ttagggctag ggattttttg ttttttattt agcgcggctt
     1081 cattcagacg atcatccaaa caatcgttaa aaatttttga tttccctatc caccttttgt
     1141 ttgtttaacc gagttaccca cagatttaca gttgcgttat gttttttaaa atgcaaggaa
     1201 tgtagttaga cgtaccgtta acacctgaat tgtttttaaa caaacaaaag ggtaaaatat
     1261 aaataatgat tgaccccaat agtcaaacaa ccgacaagca tctacaagac ctgcgcgaga
     1321 aggaagcgga agagttggct caaacccttt ccggccgcta cggacttccc tatctcgatc
     1381 tttcaaaaac agctataaat accgacgctc tacgccttat ccccgaagat gaagctcgcc
     1441 tcgccgccgt cgcggccttt aaagtagtca acaaaactct tagtttggcc attttggcgc
     1501 ccaataaacc agaagcccaa gcggtaatta aagatttgga gggcaaaaat tttaaagtag
     1561 aacttttcat ggtttctgaa gttggcctgg aaagcgcttg ggctcgctac agcgaagttt
     1621 cgctctccga aaaaactaaa gccggcctga tagaaatttc caatgaagat gtcacgcgtt
     1681 tgactgaaca attcaaaact ctaactgaca tcaaaaccga gatagacaaa gagagcgggc
     1741 tggcgctaag tcaaggcggc atttccggca tcttggaagt cattttggct ggcggtttgg
     1801 tgactgatgc ctctgacatt cacattgaac cggaacaaga aacaattcgc ttgcgctatc
     1861 ggctggacgg tgttttgcaa gatgtggctg actttccgct taaaattcac cgccaaatta
     1921 tttcacgcct aaagttggtt tccggcatga agttaaacgt caaagaagcc gcccaagacg
     1981 gtcgtttctc catcgcgttg gagaatagcg atattgaaat ccgctcatcg gttattcctt
     2041 cggcttacgg cgaatcagtg gttatgcgta tcctaaaccc aaaaacgatt gagctgactt
     2101 ttgccaaatt gggcgtggaa acaaaactgt ttgaaatttt taatcgtgaa atccacaagc
     2161 cgaacggcct agttctcctg actggcccga ccggttcagg taaaacaacc acgctttatg
     2221 cctttttgcg cgaaatcaat tcgccggaaa ataaagttat cacgattgaa gacccgattg
     2281 aataccatct gggaggtata aaccaaaccc aagtcaacaa aaaacagggt tatactttct
     2341 tgttgggttt gcgcagcgct ttgcgccaag acccagacgt catcatggtt ggagaaattc
     2401 gcgacagcga aaccgccaag accgcggttc aatcttcttt gactggccac ttagtctttt
     2461 ccaccctgca caccaacaac gcccagggcg ctatcccccg cttgattgat ctggaggtta
     2521 atccaaagat tatcacttcg tcgttgacac tctctattgc ccaacgtctg ttgcgcaaac
     2581 tttgtccgca ctgcaaaaaa gaaatcgccc ccaccgaaag cgagaaggag ctacttagcg
     2641 ccgtaattca aagtatcaag gaaaagagat cggaattggt gccagaaaat tggcaaggcg
     2701 aaattaccga ggcaaacaaa attttcgtag ccggtggctg tgaacaatgc aatggtatcg
     2761 gttacaaggg gcgagaagga gtctttgagg cgattgtaat ggacgacgcg gtcgcggccg
     2821 ccaccattac caacccaagc gaaaaagaaa taaaaatagc cgccaaaccc caaggaatcc
     2881 ttgatatgcg gcaagatggt atcttgaaag tcattaccgg acgcaccacc ctggaagagc
     2941 tgtcgcgcgt cgttgattta tacgaagaaa ttatttaaac cgttctttgc aaacagagaa
     3001 acgaatcaag actacaaacc tccaagcaat ccctttggat gtgcatctaa agttagataa
     3061 ttcctcaagg atagcttcag taaaactcac tgctaaggtg aaaaccgaag cgtcctcttg
     3121 aaagaacccg ttaggtctgt aaacaggcct agattgcttg gaggtttgta agcttgataa
     3181 aaaaacaaaa aagaaacgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3301 ccgaacacct gcttttttat ggtccccctg gtctcggcaa gactaccctt gcttacttgg
     3361 tcgccaaaga gatgaacgcg caaattaaag tgacctccgg tccggccatt gaaaaagttg
     3421 gcgacttggc ctctatctta accaacttgt ccgccggcga tattttattt attgatgaag
     3481 ttcatcgcct aaacaaaatg attgaggaag tgctctatcc ggcgatggag tccggccacc
     3541 tgaatattat tattggcaag gggccggggg cgcgcgttat ccaaattgac ttgccaccgt
     3601 ttacgctatt ggcagccacc acgaggatcg ccgccatctc ttcgccctta cgctcacgtt
     3661 tttccggcgg tactttcaaa ttggaattct acaccaatga agaaataagc gagataatca
     3721 agcggtcagc ccaaattctc ggaattgaac ttggagatga gagcgccttt tccatcgcca
     3781 aacgcagtcg ttttactcct cgcatcgcca accacctttt gaaacgctgc cgcgactacg
     3841 cccaagtaac caagaaggct atctcgccca aaaccaccaa cgaagctttg agcttgatgg
     3901 gaatagacga acttggtcta aacgaacccg accgtaaaat catagaggta attattgata
     3961 agtttggcgg tgggccggtc ggcgttggca caatcgcgac cgccctgtct gaagaagaag
     4021 ccactattga ggatgtctac gaaccatacc tgatccaact cggttttctg gagcgcactc
     4081 cccgcggtcg agtggtaacc gacaaaggct acgagcatct tggatttgat ttgcccccga
     4141 aaaaccaacc taagctcatt taggcttttc cctggtttat cttcttaatc tttacatttc
     4201 aatttttaaa ttttaaagta tctttatgtc tggtcacaat aaatggacac aaataaaaca
     4261 caagaaaggc gctgaagata tcaagcgtag taaacttttt tcaatgctgg ttcggacaat
     4321 caacgtggag gctagattgg ccaacggtaa ccgcgaggcg ccggggctaa aagcggcaat
     4381 cgtgcgcgcc aaggcggcca atatgcctct cgataatatt gaacgcgcga ttgaaaaagg
     4441 ttccggggcc ggcgccgaat cttttgaaga agtcgtttat gaggcctacg gtccgggcgg
     4501 tgtcgctttg cttattgttg gtcttaccga caacaaaaac cgcaccaccc ctgaaattaa
     4561 acaccttttg tctaagcatg gcatcagtct cagcccacag ggcgccacac aatgggcctt
     4621 tattaaaaaa gaagacgaat ggcaggcgca aaacaccctg cccttggcaa agactgagca
     4681 agaaaagttg caagccctcc ttgaggccct aggcgaacac gaggatatca aaaatatttt
     4741 ccataatgcc gacgaaatcg cttaaaatcc tggggattga ccccggttat gaccgcctcg
     4801 gtttagcggt tattgaaaaa agccaaaccg gcttgaaact gctttattcc acctgtcttc
     4861 aaacaaataa aaaagatagc ctctcctcgc gtttcttatt gttgggccga gaactaaaaa
     4921 aaataataaa aaaagaaaaa ccagacgtgg tcgcgttgga gactttatac ttctcagtta
     4981 accaaaaaac agccagcaag gtagccgagg tcagaggaat gattgcttat atttgcgccg
     5041 aatcaggttt gccaatttct gaatataacc caatgcaagt aaaatcttgc gtggccggtt
     5101 ggggcggcgc caacaaaaaa caaatcattg cattggtcaa aaagcaaatt gttgtgccgg
     5161 ccaaggtgaa aatagatgat gaatatgacg ccatcgcgat tgctttgact cactcggtta
     5221 tggccaaaat gttgtccttg tgaaaaaatt ttggtttttt acaagccccc aaaaccttaa
     5281 aaataaaggc aaaatagtcc tctaatcatc ttatccacag acgggcccaa agggtattgc
     5341 aaaaattttt acttatgata aaaattaaga acttgaaaat aaacaaaggt cgtgattggt
     5401 ttattattag caacaagtaa aataaaatgg acgagctata caacgatgac gtcttaggtg
     5461 gtgtcgcctt aagtgacgac gactccttcg gcgatgatga agagctggaa aatctggatg
     5521 atgaagtgga gacagacgat gaagacgact ccttcggcgg cagtgatgac gatgacgaag
     5581 aataaaatca gttcataaac caaatcaaaa gagagggctt tttggaccct ctcttttgat
     5641 ttccgtgaca aagtttttta tttaagttca atcttaagaa aatttttctt accaatcttc
     5701 aaagtgacgt ctttttctat tttggctgtt ggttccgtta ttttttctcc attaatctca
     5761 cttagaccgc cccccgcgac caaacgccga aaatcgcttt ttgatttaac caagttattt
     5821 ttcaacaaaa tatccattag caatgtgcct ttgacggcgt ttattatttt tatatctgcc
     5881 gggatttctt ttttttgaaa agcggtaaca aaaaaatttt gggctttgtc agcttcctta
     5941 tcaccgtgat aaagtttcac tatttcccaa gccaaccgca tttttaggtc acgcggatta
     6001 gcgccagcgt gtaattcttt tttaatattc tcaatttcag caagaggtag gtcggtacaa
     6061 tccacaaaac actgaataat tgcttcgtcg ggcaaggcca ttgttttacc aaacatgctc
     6121 gccggcgatt catcaaggcc aatccccgtt ccaagactct tactcattaa tttttcacca
     6181 gtcactggat tttcaagcaa ggttgtggtc acaactattt tttctttttg tttataggcc
     6241 tgcaaaagag ttcgccccgc caacatatta aaagtctggt cagtaccgcc tatttcaata
     6301 tctgcgtcta aagcaattga gtcatatcct tgcattagtg gataaaaaaa ctcatgcaca
     6361 tataatggtt tttgttcttt taatcttttt tcaaaattat ccctctctaa catttgttgc
     6421 acagtaaagt ttgaagccag ttccaccaga tcattaaaat taagtttaga tagccattta
     6481 gaattaagaa caaattctac tgggtttttt ttatctttaa agtccaatat tttaccgatc
     6541 tgttttttaa aatccttcag gtttttcttt acttcatttt tagttaaacg ctgtctagca
     6601 gcctttttgt cgctaggatc gccaatcatc gccgtaaaat caccaactaa aacaatgatc
     6661 ttgtgaccca attggtgcaa acgacgcagc aaaaggtagt tagtagcgtg tcccaaatga
     6721 acatatgtag ccgtcggatc aatccccatg taaattctaa gggggcgatt agagtgtaaa
     6781 agttcagcaa atttttcaga agtcggataa atcttagaaa ttgaacgagt taaaacttct
     6841 tgtattcttt cttctttctc ttttaaattc atagaataaa aatatcacat attagctttt
     6901 tgcgccatcc atgctaatat ttagccatgt ctaaaagaaa acagcaaatt acccgtaatc
     6961 ttatcaggat tatcttggta gttttaatta tcggtttagt ttccggcggg gtcactacaa
     7021 tctgggccat ttctttgcca gtgcctgatt ttgaaacctt ttttgaaaaa aatgctattt
     7081 tgtcgactgc tcttgacttt cgc
//
LOCUS       DAOOLO010000029         6750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_13443_length_6750_cov_6.059597, whole genome shotgun sequence.
ACCESSION   DAOOLO010000029 DAOOLO010000000
VERSION     DAOOLO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13443_length_6750_cov_6.059597"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..123)
                     /locus_tag="PLH96_02360"
     tRNA            complement(53..123)
                     /locus_tag="PLH96_02360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Gln,seq:ctg)
     gene            complement(167..643)
                     /locus_tag="PLH96_02365"
     CDS             complement(167..643)
                     /locus_tag="PLH96_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60636.1"
                     /translation="MAKDEVIKNMVYNSEVESKIIKILKQDKSVVLIGPSNSGKTFFA
                     KNKLIPKLEMEGCRVVYFEDGNCIEEKDCDFVIFDEAETLIDQKIIENKESQLYSEKY
                     LQKVQNWTNKYSVFTQPTVFIISRDKEDIEFLLDNLKEVGGKKCVCLEWEDFKPSF"
     gene            767..952
                     /locus_tag="PLH96_02370"
     CDS             767..952
                     /locus_tag="PLH96_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60637.1"
                     /translation="MAKLEKFPNWGQAQEVAEEFSRYLDSLNLKKEEKERVRDFTQRI
                     RVLTSVEGINMGTLNED"
     gene            1082..2188
                     /locus_tag="PLH96_02375"
     CDS             1082..2188
                     /locus_tag="PLH96_02375"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPB60638.1"
                     /translation="MSKFFKISIYLLLSVAGLAAIVAAIYFFLINRAEPTTYQKPANN
                     LVPNKTNPQNQTGQKILSTDYHVYQTFNNCAPAALSMALSYFGVNASQEDLANDLRPN
                     HNLTGQNDDKSTPPNELAAKAKDYGLVVFYRPAGNIEKLKALINLDLPIVVRTLLYPD
                     QDYAHYRVIKGYDETTNEIIQDDSLEGKNLRFAYQEFERLWQPFNNAYLVIARPEQQA
                     AIEKILGEDLTENIAWQKAVALAQNQLSQNPTDINANLNLAVANYYLGDFTGSVSAFE
                     KVAANLPKHFLWYQNEPIKAYFALRQYDKVFALTDKIFADNNKADSELYLLRGQSYLA
                     QDQKDEARAEFEKAVFYNKNYLPAQEALSSLDDD"
     gene            2239..2799
                     /locus_tag="PLH96_02380"
     CDS             2239..2799
                     /locus_tag="PLH96_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPB60639.1"
                     /translation="MIILDCEPKTDEELVALAVSNPDFFACLVKKYEPSLRRYSRRLI
                     GQRDDEDDLLQDVFLKCYLNLNDFDQNLKFSSWLYRITHNHLVDYLKNKSRLKFTGEE
                     AEKFLQKIDGGEDSSSLAKQNEASHLVDKILEKIDAKAREILVLRYLEDKSYEEISDI
                     LKIPLGTVAARLNRAKLKFKKATLNK"
     gene            2805..3584
                     /locus_tag="PLH96_02385"
     CDS             2805..3584
                     /locus_tag="PLH96_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60640.1"
                     /translation="MTDLSKNLIDKIKEEKIAPKARWRFLAKDKLLWLLFGISIIIGG
                     LSFGVILYFAGDNDLVTIFWAKQGFVNQLLSTLPHIWLILIVIISYLASLNFSATKTG
                     YRLRPRNVIALSLMTSLIIGATAYAFGFGAYLDTRFGQALPFYKSLGERRFEFWLQPE
                     AGRLAGEIIIYKDNLSFQLKDPQQKTWVIYTNQETFWAPPIERGTGQLVRVIGEQTAD
                     CEFSATGIYPWQRANFGPHPGSRMMMPRHLERNFSGNRNNQ"
     gene            3615..3995
                     /locus_tag="PLH96_02390"
     CDS             3615..3995
                     /locus_tag="PLH96_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60641.1"
                     /translation="MKKTIIVGSLLAITLLASTAMAYQGNPGQSGPNYSPERHENMIK
                     AMETKNYETWKELMGNRGRVTQVINEDNFNLFAEAWQLTREGKFEEAAEIRQELGLGQ
                     GPRQMFGQSRNGFGRNMSKCPMTD"
     gene            4115..4615
                     /locus_tag="PLH96_02395"
     CDS             4115..4615
                     /locus_tag="PLH96_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPB60642.1"
                     /translation="MLRKKVFWLIFLIIVALITLFFYPQTVLTPAQNIKNYFQPPTYD
                     GSLVRINDISIPVELATSTEAVTKGLSGRPALDENEGMLFVFDLPYRYRFWMPDMNFP
                     LDIIWLENGQIADITENATEDFDPLAPVFYQPNVPVRYVLEVNAGFVQKHNLKIGDQA
                     TLINIE"
     gene            4617..5669
                     /locus_tag="PLH96_02400"
     CDS             4617..5669
                     /locus_tag="PLH96_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPB60643.1"
                     /translation="MQITPAQSSDRESWNIFVKNNYPPVGVFMQTWEWGEFKKDWGKK
                     IERFFVTDGDQLIAVFMLAEHSLPFGFSYGYLPRGPVLAKTLDQQKINAVFSFIRDWA
                     IQKHSHLVFLRLEPPVQEEVVAELGKKHFLRPDYYIQPRYNLTLDLTKPEEEILASFH
                     SSTRSNLNRAERRGVTVTLKNHQQDVSQSDFWQMTKDTISRNSGKNLYPPRTYFNSMF
                     KVLPALGNETSPDQLSIGTFCGHQHDKPAATHYVLFFGDTATYIYGASATECLKSKVT
                     TYLHWQAAREAKARGFKYYDLGGIDEKLWPSITTFKRQFGGLEFAYVGLIDIPLQPRL
                     YKLYNFLKKLRHLGKS"
     gene            complement(5755..6531)
                     /locus_tag="PLH96_02405"
     CDS             complement(5755..6531)
                     /locus_tag="PLH96_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPB60644.1"
                     /translation="MNSALDEVKSKVDISISFKTDAPEEDIEALKNELALLPEVKEAT
                     YSSRESELANFRERHKDNEILMQSLDEVGNPFGARLNILAVDPGQYDVITRFVENKNS
                     PELGGDQIIDQVSYKKNVVDKLLRLIETSQKVSFGISFVLALLAILVVFNTVSLAIYT
                     SREEISVMRLVGAGNFYVKGPFVIEGIISGILSSFIALVALYPAAIWVRDLTAGVYGG
                     VNLVSFYVSNFGKIFLILFLTGIILGLIASFWATRRYAKV"
BASE COUNT         2042 a         1557 c         1289 g         1862 t
ORIGIN      
        1 tattttgctc tttattcata attttagggc ttgcactgag cttgccgaag tgctgccgga
       61 cttggactcg aaccaagata ccatcctcca gagggatgtg tcctaccatt agacgatccg
      121 gcattatgtt tcttaattta tcacaagttt ggaataaaat ccatgactaa aaacttggct
      181 taaaatcttc ccattccaaa caaacacatt tctttcctcc aacttctttt aagttgtcta
      241 acaaaaattc tatatcttct ttatcgcgac ttatgataaa aaccgtcggt tgagtaaaaa
      301 cagaatattt attagtccaa ttctgaactt tttgcaaata tttttccgaa tacagctggc
      361 tttccttgtt ttcaataatt ttttgatcaa tcaaagtttc tgcttcatca aaaatcacaa
      421 agtcacaatc tttttcttca atacagtttc catcttcaaa ataaacaact ctgcacccct
      481 ccatctctag tttgggaatt agtttatttt tagcaaaaaa ggttttcccc gagtttgaag
      541 ggccaatcaa aacaactgat ttgtcttgtt ttaaaatttt tattattttg ctttccactt
      601 ctgaattata aaccatattt ttaataactt catcttttgc caaattcgtc atttaaacct
      661 gtggggtaac gcaaaaacgt tgaaacatcg ccttccattc tttacattta ctacagttta
      721 tgttattagt tcattaggtt ggaaccttag aagggaggtg acaaaattgg ccaaacttga
      781 aaaattccca aactggggac aggcccaaga ggttgccgag gaattttctc ggtatcttga
      841 ctctctcaac ctaaaaaagg aggagaagga acgggtcagg gacttcaccc aaagaattcg
      901 ggtgttaacc agcgtggagg gaataaatat ggggacctta aatgaagact aacacccgct
      961 caaaaacaac agactcgttt ctgttgtgga tgaagcgggt ttgttgtttg ctcgattttg
     1021 ataactcctt taaacttcta ttgccttttt aaccgaaggc aggtataaaa gattgtaagg
     1081 tatgtctaaa tttttcaaaa tctctattta tttgctctta tcagtcgccg gcttagcggc
     1141 aattgtcgcg gctatttatt ttttcctaat taatcgcgct gaaccaacca cctatcaaaa
     1201 accggccaat aacttagtcc ccaataaaac aaacccccag aaccaaaccg ggcaaaaaat
     1261 tttatcaacc gattatcacg tttatcaaac tttcaataac tgcgcgccgg ccgccctctc
     1321 catggccctg tcttactttg gggtcaacgc aagtcaagaa gacctggcca atgacttacg
     1381 acctaatcac aacctcactg gccagaacga tgacaaatcg acccccccga acgaattggc
     1441 agccaaagcc aaagactatg gccttgttgt cttctatcgt ccggccggta acattgaaaa
     1501 actgaaagcc ctaattaatc tggacttgcc gattgtcgtc cgcacactcc tctatcctga
     1561 ccaggattac gctcattacc gagtcattaa aggttatgat gaaacaacca acgagataat
     1621 ccaggacgac tcgctagaag gcaaaaactt gcgttttgct taccaagaat ttgaaaggtt
     1681 gtggcaacca ttcaataatg cttatttagt tattgcccga cctgaacagc aagcagcgat
     1741 agaaaaaatt ttaggcgaag acctaactga aaatattgct tggcaaaaag ccgtcgcctt
     1801 ggctcaaaac caattgagtc aaaatcccac ggatataaac gccaacctta atttggccgt
     1861 cgccaattat taccttggcg atttcaccgg ttcagtgtcc gcctttgaga aggtcgccgc
     1921 caatttgcca aaacactttc tatggtacca aaacgaacca atcaaagctt attttgccct
     1981 gagacaatac gacaaagttt ttgccttgac ggataaaatc tttgccgaca acaacaaggc
     2041 cgacagcgaa ctctatcttt tgcgtggcca aagttattta gcccaagacc aaaaggacga
     2101 ggcgcgagcc gaatttgaaa aggccgtctt ctacaataaa aattatttgc cggctcaaga
     2161 agccttaagc tctcttgatg acgattaaaa actccgaaac gcaaggaaaa ttgccataaa
     2221 tcgtattata ataaacagat gatcatttta gattgtgagc ccaaaaccga tgaagagttg
     2281 gttgctctgg ccgtgagcaa ccccgatttt tttgcctgcc tggtaaaaaa atatgagcca
     2341 tcgcttaggc gttacagccg tcgactcatt ggccagcgtg atgacgaaga cgatttactg
     2401 caagatgttt ttttaaagtg ctatcttaat ctgaacgact ttgaccaaaa tctgaagttt
     2461 tcttcctggc tctatcggat tactcacaac catctggtag actatcttaa gaacaagagc
     2521 cgtctgaaat ttacaggaga agaggctgaa aaatttctgc aaaaaataga cggcggcgaa
     2581 gatagctcgt ccctcgccaa acaaaacgaa gccagccatc tggtagataa aatcctagaa
     2641 aaaattgacg ccaaagccag agaaatttta gtcctgcgtt acttggaaga caagagctac
     2701 gaagaaatct ccgacattct aaaaatcccc cttgggactg tagctgccag actgaaccga
     2761 gccaaactca aattcaaaaa agctaccctt aataaataaa aaaaatgact gacttaagta
     2821 aaaatttaat agacaaaatc aaagaagaaa aaatcgcgcc taaggctcgt tggcggtttt
     2881 tggccaagga taaattgttg tggctactgt ttggaatttc tatcatcatt ggcggtttat
     2941 catttggtgt aatcctttat tttgccggcg acaatgattt ggtaactatt ttttgggcca
     3001 aacaaggttt tgtcaaccaa ctgttaagca ccttgcccca catctggtta attttaattg
     3061 ttatcatctc ctatctggca agcctaaact tttcggccac caagacgggc tatcgcctaa
     3121 ggcctcgtaa tgtaatcgcc ttaagcttga tgactagttt aatcattggc gcaaccgcct
     3181 atgcctttgg ttttggcgca taccttgata cccgttttgg ccaggcacta cctttttata
     3241 aaagcctcgg tgaacgacgg tttgaatttt ggctccaacc agaagctggt cgtttggccg
     3301 gcgaaataat catctataag gataacctta gctttcaact aaaagatccc caacaaaaga
     3361 cttgggtaat ttataccaac caagaaactt tttgggctcc accgattgag cgcggaactg
     3421 gacagttagt tagagtgatc ggcgaacaaa cggccgattg tgagtttagc gccaccggca
     3481 tttatccttg gcagagagcc aattttggtc ctcacccagg ttcacgaatg atgatgccca
     3541 gacatcttga aagaaatttt tccggcaatc gtaataatca gtaaaggtcg tattacaaag
     3601 aaaatattta ccttatgaaa aaaacaatca ttgtcggttc tttgctggca ataactcttc
     3661 ttgcttccac cgcaatggct tatcagggca atccgggcca atctggtccc aactattccc
     3721 ccgaaagaca cgagaatatg atcaaggcca tggaaacaaa aaactatgag acctggaaag
     3781 aactgatggg caatcgcggt cgggttaccc aagtcatcaa cgaggataac ttcaatctct
     3841 ttgccgaagc ctggcaacta acgcgcgaag gaaagtttga agaagcggcc gaaatcagac
     3901 aggagcttgg gctaggccag ggaccacgcc agatgtttgg tcaatcaaga aacggctttg
     3961 gtcgaaatat gtccaaatgc ccgatgacag attaaaaaat atcaaacgta aagaaacagg
     4021 ccatcaaatg ggcctgtttc ttggtttaaa ttcttatagt tttaattttg cattatgcta
     4081 taataaactg gaccgataat ttatggtttt taatttgctt agaaaaaagg ttttttggct
     4141 gatttttctg ataatcgtcg cgctcataac tctgtttttt taccctcaaa ccgtcttgac
     4201 tcccgctcaa aatataaaaa actatttcca accgccgact tatgacggat ctttagtgcg
     4261 gattaatgat atttctatcc cggttgaact ggccacctcc accgaagcgg tgactaaggg
     4321 tttatccggt cgccccgctt tagatgagaa cgagggaatg ctttttgttt ttgacctgcc
     4381 ttatcgttac cgcttttgga tgcccgatat gaacttcccc ctagacatta tttggctaga
     4441 aaacggccaa atcgctgaca tcaccgaaaa cgcgactgag gattttgacc cgctcgcgcc
     4501 agttttttat caaccaaacg tgccggttcg ttacgtcttg gaagttaatg ccggcttcgt
     4561 ccaaaaacac aacctgaaaa tcggcgacca ggcaacgtta atcaacatag agtaaaatgc
     4621 aaattactcc tgcccaatca agcgaccgag agagctggaa tatttttgtc aaaaacaatt
     4681 acccaccggt aggcgtcttt atgcaaacct gggaatgggg cgagttcaaa aaagactggg
     4741 gtaaaaaaat tgaacgtttt tttgttaccg atggcgacca actaatcgct gtctttatgc
     4801 tggccgaaca ttctctgcct tttggttttt cttatggcta tttgccacgc ggacccgtcc
     4861 tggccaaaac tctggaccaa caaaaaatca acgcagtttt ttcttttatt agagattggg
     4921 cgattcaaaa acacagtcac ctcgtttttt tacgtctgga accgccggtt caagaagaag
     4981 tcgtcgccga gctagggaag aagcattttt tacgccctga ctattacatt caaccaaggt
     5041 ataatctgac cttagaccta acaaaaccgg aagaagaaat tttagcttct tttcactcct
     5101 ctactcgttc caatctaaac cgagcggaaa ggcgaggtgt caccgtcacc ttgaaaaacc
     5161 atcagcaaga cgtaagccaa agcgatttct ggcagatgac caaggatacg attagccgca
     5221 acagtggcaa aaatctctac ccgccgcgaa cttattttaa ttcaatgttt aaagttttgc
     5281 cggcgcttgg aaatgaaact agccccgatc aactttccat cggcaccttt tgcggccacc
     5341 aacacgacaa gccggccgcc actcactatg tgctcttctt tggcgacact gccacctata
     5401 tttatggcgc ttctgccacc gaatgcctta aatccaaagt tacgacttat ctgcattggc
     5461 aagcagcgcg tgaggccaag gcgcgcggct tcaaatatta cgacttgggc gggattgatg
     5521 aaaaactttg gccgtctatt accactttta agcggcaatt cggcggcctg gaattcgctt
     5581 atgtcggtct gattgatatt ccgcttcaac cacgactcta taagctttac aatttcttga
     5641 aaaaactccg acacttggga aaaagttagg ggaggagcag aatttgaaac aaagaattaa
     5701 aactcccgcc caagcaacga agttgttttg gcgggagttt tcttatactg tttgctatac
     5761 tttcgcgtat cgcctcgtcg cccagaaact ggcaatcaag cccaagataa ttccagtcaa
     5821 aaacaggatt aagaaaattt tgccaaaatt ggacacatag aaagaaacaa gattaacacc
     5881 gccataaacg cctgcggtca ggtcacgcac ccaaatcgcc gccggataca aagcgactag
     5941 ggcgataaac gatgacagaa tgccggaaat aatgccttca attacgaacg gccctttgac
     6001 ataaaaattt ccggctccca ccaagcgcat caccgaaatt tcttctcgcg aagtgtaaat
     6061 cgccaatgag acagtattaa aaacgaccag aatggccaaa agtgccagca cgaaactaat
     6121 tccaaaactc actttctgac tggtttcaat caagcgcaac agcttgtcta ccacgttttt
     6181 cttataactg acttgatcaa taatctgatc accacccaat tcaggactat ttttgttttc
     6241 tacaaaacga gtaatcacat catactgacc ggggtcaacg gccaaaatgt ttaatcgcgc
     6301 cccaaacgga ttaccaactt catcaagcga ctgcattaaa atctcgttat ctttgtgtct
     6361 ttcccgaaaa ttggccaatt cgctctcacg cgaagaataa gtggcttctt tgacttccgg
     6421 caaaagcgcc aattcatttt ttagcgcttc aatgtcttct tctggcgcgt cagtcttgaa
     6481 cgaaatactg atatccacct tgcttttgac ttcatctagc gcagaattca tataattatt
     6541 ggccagaaac aaaccaccca ccacaaagag agtcaccgac atggccatta cagatgcgaa
     6601 agagaccaag ccatttcgcc aaaaattgat aaatccaaat ttgaaaattc tttttgtatt
     6661 tatccagaac atattgttaa ctcaaaattt aaaactcaaa acgcaaaact agattagact
     6721 tttgtttttt gcttttttgt ttaacttttg
//
LOCUS       DAOOLO010000030         6512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_14142_length_6512_cov_7.678798, whole genome shotgun sequence.
ACCESSION   DAOOLO010000030 DAOOLO010000000
VERSION     DAOOLO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14142_length_6512_cov_7.678798"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1114)
                     /locus_tag="PLH96_02410"
     CDS             complement(329..1114)
                     /locus_tag="PLH96_02410"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPB60645.1"
                     /translation="MFSQVTIFSAAILGVIEGLTEFLPVSSTGHLILVSRLLGLSDSE
                     FIKSFEVVIQLGAIMAVAILYWRQFLVNRRVLARVLVAFGPTALIGLLAYPFVKQYLL
                     GSVVVTLAALFIGGLFLIFYEKKVGEKENPLNSIEDLSFRQAVYIGLAQAVAIVPGVS
                     RSAATIVGGLALGLSRRAIVEFSFLLAVPTMVAAVGLDLLKTSGDFTTEEYGLLVIGL
                     VTSFAVAWLAVKTFLAYIQKHDFVVFGVYRLVLSLILAIAFFS"
     gene            1168..1752
                     /locus_tag="PLH96_02415"
     CDS             1168..1752
                     /locus_tag="PLH96_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60646.1"
                     /translation="MLKRINPKIKENVENVRGLHDAIWGDFSALRGDVSKIYGDVSLH
                     KINGNVSGINGNIAGLFGNVSALRGDVSNIRGNTTHLKGDVSNISGDISGLIGDPSHI
                     RGDVTNIRGDLSKITGNVSDLVGDVSDLRGDVTNISGNVTNISGDTSHLSGNVSNLIG
                     EISSLEGDATGIEGDATGLVGRIDKQLEKEEPTF"
     gene            complement(1749..2261)
                     /locus_tag="PLH96_02420"
     CDS             complement(1749..2261)
                     /locus_tag="PLH96_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60647.1"
                     /translation="MTLGTHAIIGAAIGSVFADKPGTALALGWVSHYFFDFVPHWDYH
                     LRSAKVDLENPLNNDLSRGKDFYYDLAKIGFDALLGLALGILFFVNLARPEKLVAVVA
                     GIIGALLPDFLQFVYIRLRREPFIALQKIHGWAHSSYRELNKKPSLGFLLQLSIILAA
                     FLVGNWRLFR"
     gene            complement(2263..3321)
                     /locus_tag="PLH96_02425"
     CDS             complement(2263..3321)
                     /locus_tag="PLH96_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60648.1"
                     /translation="MSRKVKVVLNILVLLLAMAGLFFLGGFWALRLGLTKTAGKTDFY
                     DRYFIGFYQNKKTVDGMAKADDLCKLAVISRFQPSVARGLFEIMDQANYGVVLNRAIA
                     AQEIYLTQSEDYRQALSVCRGDWNFSAQNDPADWRALPEWQTLREAIVKDAEVINLAA
                     QKAGGDARLLVSVLTVEQLRLFTSEREVFKQIFQPLKILGTQSKFSWGVMGMKEETAE
                     MVENNLKDKNSPFYLGPQFENLLDFSATEATDKESARFLRLTDQHDHSYAYLYAALFL
                     KQIMAQWEKAGYNIDDRPEILATLFNLGFTKSAPKAEPAAGGAEIEIGGKAYSFGGLA
                     FQFYYSGELANDFPLKIN"
     gene            complement(3371..4069)
                     /locus_tag="PLH96_02430"
     CDS             complement(3371..4069)
                     /locus_tag="PLH96_02430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPB60649.1"
                     /translation="MRKDRFFIPTDYSGEFLVWRDRELAHQLKNVLRLDGDKVVFLFN
                     GRGEEATVSLAEIKDEDLRFKILSVQSAKELGNKITLYVSLLKRDNFEFVVQKATEAG
                     VLAIVPIVAARTVKQGDKTERWQIIAKEAAEQSGRLIVPKVCEALSFDEAIRVAAESH
                     ELNILFDLNGAKLKKVDGNIGIFVGPEGGFSPAEIGLAQEKGFSKFSLGDFTLRAETA
                     AIIGTFVISGGWRP"
     gene            complement(4075..4581)
                     /locus_tag="PLH96_02435"
     CDS             complement(4075..4581)
                     /locus_tag="PLH96_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60650.1"
                     /translation="MAKRLVDIDMVKDEPISEGGENRKSSGGHIITNILVVLVIVALA
                     AAGWFYWKYTETQKFLTGAGTEAEMEQEIKEVTAAVGKLMILPDEKPQIATVVDPDTL
                     VKEQPFYEGVNKGDKVLFYAEAKRAIIYSPSRGLIVNAGPVYYNEEASEQQVETQPVT
                     EETTSTDE"
     gene            complement(4647..5318)
                     /gene="rsmI"
                     /locus_tag="PLH96_02440"
     CDS             complement(4647..5318)
                     /gene="rsmI"
                     /locus_tag="PLH96_02440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB60651.1"
                     /translation="MAVYFVGTPIGNLKDITLRALEVLKTSPLVLVEKWSDSIKLLNH
                     YEIKPEKIVTYDDRNCRRVTPRLVGELKDKDAAFITSAGMPGISDPGAYLARACREAG
                     IEVVPIPGPSSLSAALAVCGFEAPFLFIGFLPKKVGQLTKTLKAAAEAGNNLVFFEAT
                     FRLEKTLKFLAENYPQTLLFVGKEMTKKFESYEVGYAEDFLEKIKENKSFTKGEFVVI
                     ANFAQ"
     gene            5643..6320
                     /locus_tag="PLH96_02445"
     CDS             5643..6320
                     /locus_tag="PLH96_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB60652.1"
                     /translation="MKILIVEDEERLAKLIKKALEKEGFAADYVTDGEAAETRIEICH
                     KDYDLVILDLMLPKKDGLEVCRSIRSKEINIPVLVLTAKFDVDDKVKTLDAGADDYLV
                     KPFSLEELVSRIRALLRRPPQTIPTVLKTQDLTLNNATRKVTRGNKEIKLTVKEFAIL
                     EYLMRNPNQVISRSQILDHLWDSDFDSFSNVIDVHVKNLRKKISNGSRRRIIETIRGI
                     GYRISTE"
BASE COUNT         1979 a         1662 c         1232 g         1639 t
ORIGIN      
        1 gggaaaactt tcgttctccc aacatgtttt ggatagtctt gctgacccaa tcactgagat
       61 tgagccaggc ctggcccgag atcttcggct caacgggcca gtcaaaacac ctggatttgc
      121 ccaggatttg gatggttgcc cgatttctaa atcggtcaac gacagcaacc ttggataggt
      181 caaggcgtat ttccaagttc ttcaagttct tcaacgaccc ctcctttctt ctgggttaat
      241 tcccccatta aacaaaaaag acctgtcggc cttgtccttt tccaagttca aatttaaaag
      301 tacttcccta tctatcatta taacatattt aactgaaaaa tgcaatcgcc aaaatgagac
      361 tcaaaacaag acgatagacc ccaaagacaa caaagtcatg tttttggata taagcgagaa
      421 aagttttgac cgcgagccaa gcaaccgcaa aactggtcac caaaccaatc accaataaac
      481 cgtattcttc cgttgtaaaa tcgcctgaag tttttagaag atctaaaccg acggccgcca
      541 ccattgttgg gacagctaac aaaaaagaaa attctacaat cgcccgccgc gacaaaccca
      601 aggccaaacc gccgacaatc gtggccgcgg atcgagacac ccccggaaca atcgccaccg
      661 cttgcgccaa accgatataa accgcttgcc ggaatgacaa gtcttcaatt gaatttagcg
      721 ggttctcttt ctcgccgact tttttctcat aaaaaatcaa aaacagaccg ccgataaaca
      781 aggccgccaa cgtgacgact acactgccaa gaagatattg tttaacaaac ggataagcca
      841 agagcccgat aagcgcggtc ggtccaaaag cgaccaaaac ccgagccaaa acgcgacgat
      901 taaccaaaaa ttggcgccaa tacaaaatag cgaccgccat aatcgccccc aactggatca
      961 ccacttcaaa acttttgata aattccgaat ccgacaaacc aagcaaacgc gaaaccaaaa
     1021 tcaaatgccc ggtagaggaa accggcaaaa attcggtcag gccttctata acgcccagaa
     1081 tcgccgccga aaaaattgta acttggctaa acatagttct tccattataa ttaatttaag
     1141 cttaaaagaa aaataaaatt tacctctatg ctaaaaagaa tcaaccctaa aataaaagaa
     1201 aacgtggaga atgtgcgcgg tttacatgac gctatctggg gcgacttttc cgccttacgc
     1261 ggcgacgtca gtaaaattta cggcgatgtt tcattgcata aaataaacgg caacgtctca
     1321 ggcatcaatg gtaatatcgc cggtcttttt ggcaacgtct cagctctgcg cggcgatgtg
     1381 agcaacattc gtggcaatac cacccacctt aaaggcgacg tgagtaatat ttccggcgat
     1441 atcagcggat taatcggcga ccccagccac atccgcggcg acgtcaccaa catccgcggc
     1501 gatttatcca agattaccgg gaacgtctca gacttggtcg gcgatgtttc agaccttcgc
     1561 ggcgacgtca ccaacatttc tggcaatgtc accaacattt ctggcgacac aagccatctg
     1621 tccggcaacg ttagcaatct tatcggcgaa atttccagcc tagaaggcga cgcgaccgga
     1681 attgaaggcg acgcgaccgg cttggttggc cgaattgaca aacagctcga aaaagaagaa
     1741 ccgaccttct aacgaaagag ccgccaatta ccaaccaaga aagccgccaa gattatggat
     1801 agttgcaaca aaaagcccaa agacggcttt ttgtttaact cgcgataaga agaatgcgcc
     1861 cagccgtgta ttttttgcaa agcaataaac ggctcccgcc ttaacctaat ataaacaaac
     1921 tgcaaaaaat ccggcaacag cgcgccgata atgccggcca caaccgccac cagcttctct
     1981 ggccgcgcca aattgacaaa aaacaaaatc cccaaggcca aaccaagcag ggcgtcaaaa
     2041 ccgattttgg ctaaatcata ataaaaatct ttacccctag acaagtcgtt gtttagcggg
     2101 ttctcaaggt ccaccttggc tgaacgcaaa tgatagtccc aatgaggcac aaagtcaaaa
     2161 aagtagtgac taacccaacc gagcgccaaa gctgttcccg gcttgtcagc aaaaacactt
     2221 ccaatcgccg cgccaataat agcatgagtt cctagcgtca tattaattta tttttaaagg
     2281 aaaatcgtta gccaattccc ccgagtaata aaactgaaag gccaacccac cgaaactgta
     2341 agccttgccc ccaatctcaa tttccgcgcc gccggccgcc ggctcggcct tgggcgccga
     2401 tttagtaaaa cccaagttaa acaaggtggc caaaatttcc ggtcggtcgt ctatattata
     2461 accggccttc tcccattgcg ccattatttg ttttaggaaa agagcggcgt aaagataggc
     2521 ataagaatga tcatgttggt cggtcaagcg aagaaatcgc gcgctttctt tgtcggtcgc
     2581 ttccgttgct gaaaaatcaa gcaagttttc aaactgtggg cccaaataaa aaggtgaatt
     2641 tttatctttt aaattgtttt ctaccatttc tgccgtttcc tctttcatcc ccattacccc
     2701 ccaagaaaat ttgctttgcg tccccaaaat ttttaatggt tgaaagattt gcttaaaaac
     2761 ttctcgctca gaagtaaaaa gacgcaactg ttctactgtc aaaactgaaa ccaaaagtct
     2821 cgcatctcct ccggccttct gggccgccaa gtttataact tcggcatctt taacaattgc
     2881 ctcgcgcaaa gtctgccatt ccggcaaggc gcgccagtcg gctggatcgt tttgagcaga
     2941 aaaattccaa tcaccgcgac aaacagacaa agcttgtcga taatcttcac tttgcgttaa
     3001 gtaaatttct tgcgcggcaa tggcgcgatt caagactaca ccataatttg cctggtccat
     3061 aatttcaaac aaaccccgcg cgactgaggg ctgaaatcgg ctgataaccg ccagcttgca
     3121 caagtcgtcc gccttagcca ttccgtcaac cgtcttctta ttttggtaaa aaccaataaa
     3181 ataacggtcg taaaaatcgg ttttgcctgc cgtcttggtc agacccaaac gaagcgccca
     3241 aaagccgccc aagaaaaaaa gaccggccat cgccagcaga aggactagaa tgttcaaaac
     3301 gactttgact tttctactca tcagaattaa attaaagcaa tttaggccaa tcaaagcaag
     3361 aagagcctgc ctaaggccgc caaccgccac ttatcacaaa agtgccaatg atagcggccg
     3421 tctcggcgcg caaagtaaaa tcacccaaac taaacttgga aaaacctttc tcttgggcca
     3481 agccaatctc ggccggcgaa aagccgcctt ccggaccaac gaaaattcca atgttaccgt
     3541 caaccttttt cagtttagcg ccattcaaat caaaaagaat attcaactcg tggctctccg
     3601 cggctacccg tatcgcttcg tcaaacgaaa gcgcctcaca aacctttggc acaattaatc
     3661 gtccgctttg ttcggccgct tctttggcga ttatttgcca acgttctgtc ttgtctcctt
     3721 gcttaacggt cctggccgcg acaatcggca cgattgccaa aactccagct tcggtggcct
     3781 tttgcaccac aaattcaaag ttgtctcttt ttaagagtga gacatagaga gtaattttgt
     3841 tgccaagttc cttggcgctt tggactgaca atattttaaa acgcaaatct tcatctttaa
     3901 tctccgccaa gctgacagtc gcctcttcgc cgcgaccatt gaacaaaaaa acaaccttgt
     3961 caccatcaag acgcaaaaca tttttcaatt ggtgggccaa ttcccgatct cgccaaacca
     4021 aaaactctcc actgtaatca gtcgggataa agaaacggtc ttttctcata aaaactactc
     4081 gtcagtgctt gttgtttctt cagtgacagg ttgagtttca acttgttgtt cgcttgcttc
     4141 ctcgttgtaa taaaccggac cagcatttac aatcaatccc cgactcgggc tgtaaattat
     4201 cgcgcgcttg gcttcagcgt aaaaaagaac tttgtcacct ttgtttacac cctcgtaaaa
     4261 aggttgttcc ttgaccagag tgtccgggtc aaccactgtt gcaatttgcg gtttttcatc
     4321 cggaagaatc atcagcttgc cgacggcagc ggtcacctct ttgatttctt gttccatttc
     4381 agcttcagtt ccggcgccag tcaaaaactt ctgagtctcg gtgtacttcc aatagaacca
     4441 cccggcggcg gccaaagcaa caataacaag caccaccaaa atatttgtaa taatatgacc
     4501 acccgatgat ttccgatttt ctccgccttc agagattggt tcatctttga ccatatcaat
     4561 atcaactagt ctttttgcca taattttttg aaattagttt gataattaat ttatcttata
     4621 tttatattat aaagtatatt ataaagtcat tgggcgaaat tggcaataac cacaaactcc
     4681 cctttggtaa aacttttgtt ttcttttatc ttttccaaaa aatcctcagc atagccgact
     4741 tcataacttt caaatttttt ggtcatctct ttgccaacga acaacagggt ttgggggtaa
     4801 ttttccgcca aaaactttag ggtcttttcc aaacggaaag tggcttcaaa aaaaaccaag
     4861 ttgttgccgg cctccgccgc cgctttcaaa gttttagtta attgcccaac ttttttaggc
     4921 aagaatccta taaaaagaaa gggggcttca aagccgcaaa ccgccaaggc ggcagagaga
     4981 gatgacggac cgggaattgg cacaacttct ataccggctt cccgacaagc gcgagccagg
     5041 taagcccccg gatcggagat ccccggcata ccggccgaag tgataaaggc cgcgtcttta
     5101 tcttttaatt cccccaccaa acgcggtgtt acgcgtcgac agttgcggtc atcgtaagtg
     5161 acaatctttt ccggcttaat ttcgtagtgg ttgagcaact taatggagtc agaccacttc
     5221 tccaccaaaa ccagcggact ggtttttaga acttccaacg cccgcaaagt aatatccttt
     5281 aggttgccaa tcggcgtccc gacaaaataa actgccatat taggataaat ttaacacaag
     5341 atttaaaaaa agtaaccccc tcgcccggct aacgtcgcaa agttcaaagg ttcttccctt
     5401 ttttgctctt agcccacgtt ccgtcttaga ccaaaaagaa caattaattt agctgctatt
     5461 gaagtttact tttcagccaa acatgttaag ttggattata ataaatggaa acagccgtca
     5521 agctaaaatc ttcattaagc attcatcaaa tcttcttaat ctttaacaaa gcgcttctct
     5581 catttcatca attcattagt taaaaacaaa aaactcatta tcaattttta actttatctt
     5641 ctatgaaaat actaatcgtc gaagacgaag aacgcctagc caaacttatc aaaaaagccc
     5701 ttgaaaaaga aggctttgcc gctgactatg tcacggacgg cgaagcggcg gaaaccagga
     5761 tagaaatttg tcataaagat tatgacttgg tcattctaga cttaatgctc cccaaaaaag
     5821 acggcctgga ggtttgccgt agcatccggt caaaggaaat taacatccca gttcttgtct
     5881 taacggccaa atttgatgta gacgataaag tcaaaaccct tgacgccggc gctgatgatt
     5941 atttggtcaa accattctcg ctggaagaat tagtttcgcg catccgagcg ctcttgcggc
     6001 gcccgccaca aaccatccca actgttttaa aaacccaaga cctaactcta aataatgcca
     6061 ctcgaaaagt aacgcgcggc aacaaagaaa ttaaactaac ggtcaaagaa ttcgccattt
     6121 tagaatattt gatgcgcaac cccaaccaag tcatcagccg cagtcaaatc ttggaccact
     6181 tgtgggattc ggattttgat tcatttagca acgtgattga tgtccacgtt aaaaatttac
     6241 gtaaaaaaat cagtaacggc agccgtcgga ggataataga aactatccgc ggtatcggtt
     6301 atcgcatctc cactgaatag cttgtttctt catcgcaaat tcaagcgaat ataataagct
     6361 aaacaagatg tttgataaaa gtaaaaataa tatcttgcgt cagattgata tcctgcataa
     6421 tattcctccg taaaaagacc gaatacaacc ttttactttt atttcctttt aactatttaa
     6481 tacttaaatc aactccacca aaacgcgccg ct
//
LOCUS       DAOOLO010000031         6466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_14273_length_6466_cov_6.564810, whole genome shotgun sequence.
ACCESSION   DAOOLO010000031 DAOOLO010000000
VERSION     DAOOLO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14273_length_6466_cov_6.564810"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /gene="ligA"
                     /locus_tag="PLH96_02450"
     CDS             <1..1882
                     /gene="ligA"
                     /locus_tag="PLH96_02450"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPB60653.1"
                     /translation="EIKYPDLVTPDSPTQRVGGEPLEKFTKVEHTVSQWSFNDVFTPE
                     EMRDFDKRIKKALGSGRVPTYVAELKIDGFKIVLTYENGFLTTAATRGNGRVGEDVTA
                     NVRTVEAIPLKLEKAVNVVVEGEIWLSKDNFDKINKEQKDKGEPLYANPRNVAAGSIR
                     QLDPKVVAARRLDNFIYDLAQADFALPPTQQAELELLAELGFKVNKHHTFCPTIEEVI
                     AFWQKWQTKKDKEPYWLDGVVVKVNEQEYQERLGYTGKAPRFATAFKFPAEQATTVVE
                     DIILQVGRQGTITPVARLRPTLLAGSTVSRATLHNEDEIKRLDVRIGDTVILQKAGDV
                     IPDIVKVLTEMRTGQEKVFKWPTELEACAPIGASAKAGGGPIERIPGQAAWRCVNKNS
                     FAQLKRKFYHFVSKTAFDIEKLGPRVIDVLLENKLVVRYDDIFKLKKGDLLALPRFAE
                     KSADNLIKAIDEKRRVSLSRLLVALSIPGVGEETAEDVAKHFGALTKIEKAGLAELQA
                     ITGVGEVVGQAIYDWFREKENQNLVKGLLAEVKVLPVKKEAGAQKLAGLKFVLTGTLP
                     TLSRDEAKMLVKQNGGEVSGSVSAKTDYVLAGEEAGSKLDKAQELGVKVIDEKEFRKM
                     LG"
     gene            1971..2264
                     /gene="gatC"
                     /locus_tag="PLH96_02455"
     CDS             1971..2264
                     /gene="gatC"
                     /locus_tag="PLH96_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPB60654.1"
                     /translation="MLTKNDINNLTNLARIDLTEEEKDKLQKDLESILGYISELQKAP
                     NVTEGHRSESYYLKNVTRSDDNPNEPGGNTEKILEEAPKRRDNLFVVKKILDN"
     gene            2366..3811
                     /gene="gatA"
                     /locus_tag="PLH96_02460"
     CDS             2366..3811
                     /gene="gatA"
                     /locus_tag="PLH96_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPB60655.1"
                     /translation="MRLNKEELDKLTIKTAHEALVRGDFSAVDLTESYLAQIKEKNEE
                     LNVYLEVFADAREQATEADRRLRKNDGVTLLTGIPLAIKDNILIEGRKCSSASKMLEN
                     YTAVYDATVIKRLKECGAVFLGRTNMDEFAMGGSTESSAFGPTKNPHDKTRVAGGSSG
                     GSASAVGGGLALAALGSDTGGSIRQPSSFCGAVGLKPTYGAVSRYGVMALASSLDQVG
                     PIAKTTEDAEILFDAIRGADNLDSNAYNLPVYPVTPAKLNIGVPYSFLNGGGVAKEVL
                     DNFNQLVEKLKQDGHVVEEVEMPNLHYSLACYYVLMPAEASTNLARFDGVRYGLHLDG
                     QDVTDDYKKTRAAGFGREVRRRIMLGTFVLSAGYYDAYYNKANQARELIKQDYLNVFN
                     QYDAIITPTAPSLAFPIGKNSNDPLQMYLEDIFTVPVNLAGVPAMSIPTGFTAPLETA
                     PKGLPLGVQVAAPERREDILFAFGRLVERLG"
     gene            3833..4405
                     /locus_tag="PLH96_02465"
     CDS             3833..4405
                     /locus_tag="PLH96_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60656.1"
                     /translation="MLSPSLINVVAIFERLASYIYGIIAYFRSEDFFELANLIWWVGL
                     TASILFVGGIVYFSLKVSAIRKERDEKVFGGKVGKLEDIKEPARNERWEKIKKKLDSD
                     NPSDWNLAIIDADKILDEMLVAMGHLGENLGERLKSVEPSDFLTLNEAWEAHKVRNQI
                     AHEDNFVLTQREAKAAIAKFEKVFQEFNYI"
     gene            4478..4549
                     /locus_tag="PLH96_02470"
     tRNA            4478..4549
                     /locus_tag="PLH96_02470"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4511..4513,aa:Met,seq:cat)
     gene            4716..4789
                     /locus_tag="PLH96_02475"
     tRNA            4716..4789
                     /locus_tag="PLH96_02475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4750..4752,aa:Met,seq:cat)
     gene            4835..4908
                     /locus_tag="PLH96_02480"
     tRNA            4835..4908
                     /locus_tag="PLH96_02480"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4869..4871,aa:Asp,seq:gtc)
     gene            complement(5077..>6466)
                     /locus_tag="PLH96_02485"
     CDS             complement(5077..>6466)
                     /locus_tag="PLH96_02485"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPB60657.1"
                     /translation="TQKDLPAPPKWWKAVGVGIIVTGMAMGTGELVMWPNLVIQYGLA
                     ILWFALVGITLQYFINQEIARATMATGESFFTSSGRLIRWSPLFWLGSVILLYIWPGW
                     ASTLGTILAALFGFGHYLWWAWASLALVLIIVFAGREAYKVLEKSLKIIVPIFVIILF
                     YISYLNLSWPDLVAALKGLFSFGYWPSGIDVNVLLGAIVFAGAGGMLNLCVSLWYRDK
                     GAGMAAFSGKIRNPINGKPEAVGAIGHTFPLTEPNLKNWRGWLSHLRFDQGMIFWFIG
                     LLTITLMSLNAFVVLKPLGITPTGTDLAVILAEIFESQLGFWGGKLYLLMAYLMLFSV
                     MWTIIDALSRVISDIIHTNAQVGPLTKIFAFSRRWSLHHLYYGLILLVVFLQAVLLPF
                     SQPLVFLLLSSVLGGFTMALYTPILLYHNNRRLAKPLRPGIISNLFLFSAFIFYSVFA
                     AISIWEVAEKIF"
BASE COUNT         1838 a         1325 c         1699 g         1604 t
ORIGIN      
        1 ggagatcaaa taccctgatt tggtcactcc tgattcaccg actcagcggg tgggaggcga
       61 accgttggaa aagttcacta aggtggaaca cacggtctca cagtggtctt ttaacgatgt
      121 ctttacgccg gaggagatgc gagactttga taaacgaatc aaaaaagcgc ttggctccgg
      181 gcgtgtgccg acctatgtgg ccgaattgaa aattgatggt ttcaaaattg ttttgactta
      241 tgaaaacggt tttcttacaa cggcggcgac acgcggcaat ggtcgggttg gcgaagacgt
      301 gacggctaac gtcagaacgg tagaggccat tccgctcaaa ttggaaaaag cggtgaatgt
      361 cgtggtggaa ggcgaaattt ggcttagtaa agacaatttt gacaaaatca acaaagagca
      421 aaaagacaag ggcgaaccgc tttatgccaa tccgcgcaat gtcgccgccg gcagtatccg
      481 tcaacttgac ccaaaagtgg tggccgctag acgtttggat aattttattt atgatttggc
      541 gcaagcggac tttgctttgc caccgacgca gcaggcagag ctggaacttt tggcggaact
      601 ggggtttaag gtcaacaaac atcacacttt ttgtcccacg attgaagagg tgattgcttt
      661 ttggcaaaaa tggcaaacta aaaaagacaa agaaccatat tggcttgacg gcgtggtggt
      721 caaagttaac gagcaagaat accaagaaag acttggttat accggcaagg cgccgcgttt
      781 cgcgacggcc ttcaaatttc cggctgaaca agccacgacg gttgtggaag acattatctt
      841 gcaagttggg cgacagggga caatcacgcc ggtagcgcgt ttgcggccaa ctttgttggc
      901 cggctccacc gtctcgcgcg cgactttgca caacgaagat gagataaagc gtctggatgt
      961 gcgaattggc gacacagtca ttttgcaaaa agccggcgat gtaattccgg acattgtcaa
     1021 agtgttgacc gaaatgcgca ccggccaaga aaaggttttt aagtggccaa cagaattgga
     1081 ggcctgcgcg cccattgggg cctcggccaa ggcgggcgga ggcccaatag aacgaatccc
     1141 cggccaggcg gcttggcggt gtgtcaataa gaattctttc gcgcaactta aacgtaaatt
     1201 ttatcacttt gtgtccaaga ccgcctttga tattgaaaaa ctcggcccgc gcgtcattga
     1261 tgtgttgttg gaaaataaat tggttgttcg ttatgacgat attttcaaac taaaaaaggg
     1321 tgatttgctg gccctgccgc gctttgcgga aaagtccgcg gataatttga ttaaggccat
     1381 agacgaaaaa cgacgggttt ctctctcgcg tttgttggtt gccttgtcta tccccggcgt
     1441 gggcgaagaa accgcggaag acgtcgctaa acattttggc gctttgacga aaatagaaaa
     1501 agccggcttg gcggaattgc aggcgataac cggcgtgggc gaggttgttg gccaagcgat
     1561 ttatgattgg tttcgcgaaa aagaaaatca aaatttggta aaaggtttgt tggcggaagt
     1621 aaaagtgttg ccggttaaaa aagaagccgg cgcccaaaaa ttggccggct taaaatttgt
     1681 gttgaccggc actttgccga ccttgtcgcg ggatgaagcc aaaatgctcg tcaagcaaaa
     1741 tggcggggag gtgtccggtt cggtttcggc taaaaccgac tatgttttgg cgggggaaga
     1801 ggccggctcc aagctggata aggcgcaaga gctcggagtc aaggtgatag acgaaaaaga
     1861 attccggaaa atgttgggct aaaccgaaac ttaaaataat caaaagtggc ctgggttgtt
     1921 tttgtttggt aggcttggaa attgtcttag ctggtgctag aatacagttt atgttaacta
     1981 aaaacgatat aaataatctg accaatttgg ccagaatcga cttgacggaa gaggaaaaag
     2041 acaagctcca aaaagaccta gagtctattt tgggttatat ttctgaatta caaaaagcgc
     2101 ccaatgtaac cgagggtcac cgttcggaaa gttattattt aaagaacgta acgcggtcgg
     2161 atgataaccc taatgaaccg ggcggaaaca ccgaaaagat tctggaagaa gcgcccaagc
     2221 gacgagacaa tttgtttgtc gtcaaaaaga ttttagacaa ttagaagtaa ggacaaaagc
     2281 aacaggaatg aaggggtgtc tagaatcaaa aatgagaaca caattttcac tttttaatct
     2341 ttaagtttta atttttaaat ttattatgcg gttaaataaa gaagagctgg ataaactgac
     2401 aatcaaaaca gcgcacgagg cgctcgtccg aggtgatttc tcggcggttg acttgacgga
     2461 aagttacttg gcgcaaatta aagaaaaaaa cgaggaactg aatgtttatt tggaggtttt
     2521 tgctgacgcg cgcgagcagg cgacagaagc cgaccggcgg ttgcgcaaaa acgatggggt
     2581 gacgcttttg accggtatcc cgttggcgat taaggataat attttgattg agggtcgtaa
     2641 atgttcctcg gcctccaaaa tgcttgaaaa ttataccgcg gtttacgacg cgaccgtgat
     2701 caaacgctta aaggagtgcg gcgcggtctt tttggggaga accaatatgg atgaattcgc
     2761 gatgggaggt tccaccgaga gctcggcttt tggtccaacc aaaaaccctc acgacaagac
     2821 aagggttgcc ggcggttcgt ctggcggttc agcgtcagcc gtgggtggcg gtttggcttt
     2881 ggcggcgctt ggctcggaca ccggcggttc tattcgccag ccctccagtt tttgcggcgc
     2941 ggttggtctg aaaccgacct atggcgctgt cagtcgttac ggcgtgatgg cattggcctc
     3001 gtcgcttgac caggttgggc cgattgccaa aacaaccgaa gacgcggaga ttctctttga
     3061 cgctatccgg ggagcggata atcttgattc caatgcctac aatttaccgg tctatccggt
     3121 cacgccggcc aaactgaata ttggcgttcc ctattctttt ttgaacggcg gtggcgtggc
     3181 caaggaggtc ttggataact tcaaccagtt ggtggaaaaa ttaaaacaag acggacatgt
     3241 cgtagaagag gtagaaatgc caaacttgca ttactcgctg gcttgttatt atgttttgat
     3301 gccagccgag gcctctacca acttggcgcg gtttgatggt gtgcgctatg ggttgcattt
     3361 ggacggtcaa gacgtaacgg atgattataa aaaaacccgg gcggctggtt ttggccgaga
     3421 ggttagacgc cggattatgc ttggcacttt tgttttgtcg gctggttatt atgacgctta
     3481 ctacaacaaa gccaatcagg cgcgtgaatt aatcaaacag gattatctga atgtctttaa
     3541 tcaatatgac gcgattatca cgccgactgc cccctcgctg gcttttccga ttggcaaaaa
     3601 tagcaacgac cccttgcaaa tgtatttgga agacatcttc accgtgccgg tgaatttggc
     3661 cggagtgccg gcgatgagca tccccaccgg ttttaccgcg ccccttgaga cagcgcccaa
     3721 aggcttgccg cttggcgttc aagtggcggc gccggagcgt cgtgaagata tcctctttgc
     3781 ttttggtcgg cttgttgaaa gacttggcta aagtgaagta taataaggaa gtatgttatc
     3841 gccttcctta ataaacgttg tcgcgatttt tgagcgcttg gcttcttata tttatggcat
     3901 cattgcctac tttaggagcg aagatttttt tgaattggca aatttgattt ggtgggttgg
     3961 tttgactgcc tctatccttt ttgttggggg gattgtttat ttttcactca aggtgagcgc
     4021 tattcgcaaa gaacgcgatg aaaaagtttt tggcggcaag gtagggaaat tagaggatat
     4081 taaagagcct gcccgaaatg aacgttggga aaaaatcaaa aagaaactgg attctgataa
     4141 cccgtctgat tggaatttgg cgattattga cgctgataaa attttggatg agatgctggt
     4201 ggctatgggc catctgggcg aaaatttggg ggaacgatta aagtcggttg aaccaagcga
     4261 ttttttgacc ttgaatgaag cttgggaagc gcataaagtt cgtaatcaaa tcgcccacga
     4321 agataatttt gttttgaccc agcgcgaagc aaaagccgca attgccaagt ttgaaaaagt
     4381 ttttcaagaa ttcaattata tttaaacaac gggcggttgt gccaattttc agaaaaactt
     4441 tgacagtttt tcataaaacg gttacaataa aaagaatagc ggagtagagt aatggttcac
     4501 tcacgaggct cataacctcg ggaagccggt tcgattccgg cctccgcgac aagaaaaaga
     4561 aaggccccaa tcggggtttt tctgttttgg aaatattgaa aaagtcccca aattaagcta
     4621 agatatgggt tagtttgtaa gttgagaagt ttgtaagttg gtaagaaaga gaaaaatggg
     4681 ggagttggtt tggaagcttg ttataattat attaggctga tgtagctcag ttggttagag
     4741 cgggcgtttc ataagcgcca ggtcgcaggt tcaagtcctg ccatcagcac aggttattgt
     4801 aaaaagtagc catttgtgta aaatatattt gtttgcggtc gtagctcaac tggttagagc
     4861 actcgcctgt cacgcgagag gttgagggtt caagtccctt cgaccgcgca ttgaaaaata
     4921 acccgacttt ttgtcgggtt attttgttgc aatcgcgctt taaacccatg aggtttttgg
     4981 gcagagatat aatttaaccg cattaacacc tcaatcaaaa ctacccgctg ttttgttgcg
     5041 tagcatgtta cgtaacactt tagagtgaag aatggtctaa aatatctttt ctgctacctc
     5101 ccaaatggaa attgccgcga aaacactata aaaaataaac gcgctgaaaa gaaaaagatt
     5161 gctaataatc ccgggccgca agggcttggc tagacggcgg ttgttgtggt aaagcaaaat
     5221 cggcgtgtag agggccatgg taaaaccgcc caagacggag cttaacaaaa gaaaaaccaa
     5281 aggttgcgaa aacggcaaga gaaccgcttg caagaatacc actaataaaa taagaccata
     5341 ataaagatga tgcaaactcc agcggcggct aaaagcgaag atttttgtta atggtccaac
     5401 ttgggcgtta gtatggataa tgtcggaaat tacccgactc aaagcgtcta taatggtcca
     5461 catgaccgaa aagagcatca gataagccat aagtagatat aatttgccgc cccagaagcc
     5521 aagttgggat tcaaaaattt ctgccagaat gactgccaaa tccgtgccgg tcggggtgat
     5581 gcccaaaggt ttgagtacaa caaaagcgtt tagactcatt aaggttattg ttaaaagacc
     5641 gatgaaccaa aaaatcattc cttggtcaaa acgcagatgg ctcagccagc cgcgccagtt
     5701 ttttagattt ggttcggtca ggggaaaagt gtgaccgatc gcgccgacag cttcaggttt
     5761 gccgttaatc gggtttctga ttttgccgct aaaggcggcc atgccggcgc ctttgtcccg
     5821 ataccaaagc gaaacacaaa ggtttaacat gccgccggcg ccggcaaaaa caatggcgcc
     5881 aagcaaaaca tttacatcta tcccgcttgg ccaataaccg aaactgaaaa gtccttttaa
     5941 ggcggcgacc aagtctggcc aagagaggtt taaatagctg atataaaaga gaattataac
     6001 aaaaatcggg acaattattt ttaaactttt ttctaggacc ttataagctt cacgtccggc
     6061 aaaaacaata attaagacta gcgctagcga agcccaagcc caccagagat aatggccaaa
     6121 accaaacagg gccgcgagga ttgtgccaag ggtgctggcc caaccgggcc agatgtagag
     6181 gagaatgact gagcccagcc aaaacaaagg tgaccagcga attaagcggc cagaggaggt
     6241 aaaaaaactt tcaccggtcg ccatggtggc gcgggcgatt tcttggttga taaaatattg
     6301 cagggtgata cccaccaacg cgaaccataa aatagccaga ccatattgga tgactaagtt
     6361 tggccacatc accagttcac cggtgcccat ggccatgccg gtaacgataa taccaacacc
     6421 aacggctttc caccatttgg gcggcgcggg gaggtctttt tgagtt
//
LOCUS       DAOOLO010000032         6315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_14693_length_6315_cov_4.589137, whole genome shotgun sequence.
ACCESSION   DAOOLO010000032 DAOOLO010000000
VERSION     DAOOLO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14693_length_6315_cov_4.589137"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLH96_02490"
     CDS             complement(<1..467)
                     /locus_tag="PLH96_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HPB60658.1"
                     /translation="MQDKQKIEEQIAALEAEMADPGFWQNKDLAQERVQEFNRLKQEL
                     AGVGKYDKGDAVMTIFAGAGGDDAEDWAAMLLAMYQKYAERQKLEFYLVHDNQNDHGG
                     YRNVTFELRGSNAYGKLKNESGVHRLVRLSPFNAKSLRHTSFALVEVIPRFAKT"
     gene            complement(681..753)
                     /locus_tag="PLH96_02495"
     tRNA            complement(681..753)
                     /locus_tag="PLH96_02495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Thr,seq:cgt)
     gene            complement(897..1274)
                     /locus_tag="PLH96_02500"
     CDS             complement(897..1274)
                     /locus_tag="PLH96_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60659.1"
                     /translation="MENNRKIKASFILVIILIVTNLLWAWAYFHERSELADQQSFERA
                     EMLNTKVVGFLKMFIDDVLLAQQEVSFDTRLKLENAVRDLNDSEALAQWDKFINSKTE
                     AEAQQEVKKLLSMLVNKVQVASE"
     gene            complement(1411..3294)
                     /locus_tag="PLH96_02505"
     CDS             complement(1411..3294)
                     /locus_tag="PLH96_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPB60660.1"
                     /translation="MKIFIKNLIFFISVLPFLAMAGTIEGDQYAWGENVGWINFGTEH
                     GGVTVTDNAITGYAWDAIYGWINLAPQNGGVLNDGAGNLSGQAWSQNGGWIDFDGVTI
                     DGTSGRFSGLAEGDIYGRINFDCNHCGVVTDWRSSSSETESQIVGSSGSMSPSLYPRT
                     FLPLAQKVWETEIVDKIKKTLAILTPDFIQTRLFGNKKSNISPGDIWQGQTPLALRYP
                     SILLSPEPERFALSSAVRSISLALKAKWQITPREVSRFALQGLPKDVVLFTNKFPQVG
                     QVLSEVGVNRMSDVKRLQGLRLAMPGFSKLIGLRKTELQNNKVALVKRVPVAELSVAE
                     KEKMPSETIFARALEERIDLKTSLTFNAKGQPLQEIQTIVGKKVDLVIKPDAPTRKIT
                     GYIVLSEPVEEKTVFRSPKNLLLAMTRFAMSELIKLEESKLAEKAFVLSEFEYYDEDN
                     DGIYSASIEAPVVAGKYKMITTMDYLNPDLGVKEINMIVVVDPEGYVFTKSGLYEARI
                     SEATVTLWWLNSQKDEYQIWPAEEYLQENPQVTGPRGTYSFLVPEGTYYITTVAPGFE
                     DYQSDLFDVTEGDGIHLNIELKPKSGWFFRMNWELVALIVIALAVIWGMVVRPMIRQR
                     NKR"
     gene            complement(3489..5258)
                     /locus_tag="PLH96_02510"
     CDS             complement(3489..5258)
                     /locus_tag="PLH96_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HPB60661.1"
                     /translation="MSGINDDITELSSLTAATTTRLVVSSILRLASLGSTILGVDQDG
                     NVIATTTSVADPSWGAITGALSAQTDLMAALAGKIDVGTTSVASILLLPNLTMTKSQI
                     SDFGSPLYSYTETDPLYAAASSTILRASYSSLSSLLDDSTHRLVTDVEKGLWNGKQDA
                     LGFVPVDSATTSMAALTSVGGLLTISGGNVGIGTTSPLYSLSVEGSSSLGNQALAGYF
                     TATSTTATSTFAGGLTVNSNSLVVDYTTGNVGLGTATPDYAFQVYIDDSNEGHVAATG
                     DWARTSDIRMKKNILTLEGALDKVMQLRGVRYDWVADEDAESSTHIGFIAQEVEALFP
                     EFVDTGASGIKGVAYGAFTPVLLQAIKEQQGIILSMTGASTTGLIFASSTNTIEDRLA
                     EITSELSDNFINYFEEKISNSFKLVKEFVAERVSAMVGYFGFVKAGKVHSLEGYETVD
                     KATGDTYCIYIKNGEVHSALGACDEISVTTSEDLSLREDGGASNEVIIDNAVPDTSFD
                     DGDETSTTTPPTEEPADETSTTTPPAEDLVDEAGTTVTETMESDLEVEPLAEESQEEP
                     TASPSADEEPPSEPNPTPDTNLD"
     gene            complement(5673..5754)
                     /locus_tag="PLH96_02515"
     tRNA            complement(5673..5754)
                     /locus_tag="PLH96_02515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5712..5714),aa:Thr,seq:ggt)
     gene            complement(5801..6058)
                     /locus_tag="PLH96_02520"
     CDS             complement(5801..6058)
                     /locus_tag="PLH96_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPB60662.1"
                     /translation="MSCGVYILKTERGYYIGSTDNLDRRIKQHLSGHTPSTKRMGSIE
                     LVFFQKFDNLKEARKIEYKLKRLKRRNYIEKIIKDGIISIG"
     gene            complement(6071..6143)
                     /locus_tag="PLH96_02525"
     tRNA            complement(6071..6143)
                     /locus_tag="PLH96_02525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6108..6110),aa:Thr,seq:ggt)
BASE COUNT         1780 a         1600 c         1191 g         1744 t
ORIGIN      
        1 gttttggcaa aacgcggaat aacctccacc agagcaaaag aggtatgccg caaacttttg
       61 gcgttgaacg gcgaaagacg gaccaaacga tggacacctg actcattttt caacttacca
      121 taagcattac tgccccgaag ctcaaaggtc acgttacgat agcccccgtg gtcattttgg
      181 ttatcatgaa cgagataaaa ttccaatttc tgccgttcag catatttctg gtacatcgca
      241 agaagcatcg ccgcccaatc ctcggcatca tcaccaccgg cgccggcaaa aatagtcatc
      301 accgcgtcgc ctttgtcata tttgcccacc cccgccaatt cttgttttag acgattaaat
      361 tcttgaaccc tttcttgcgc caaatcttta ttctgccaaa aacccggatc cgccatttcc
      421 gcctccaagg ccgctatttg ttcttcaatt ttttgtttgt cttgcatagc gcgacactga
      481 tatacgaatt catgcttgcc cgtccgaaac ttgcagttaa gcgaaggagg gcggatgata
      541 cgaatagaag gaaattaaaa tggaaaaatt aaagtgtaaa gtgatataag aaatgtaaaa
      601 tttaaaaatt aaaatggaaa atggagaacc tacatctttt ttgttttctg attttaaatt
      661 ttaaccttta cattctcatg agccgcccgt cggacttgaa ccgacaacct atggtttacg
      721 ataccattgc tctaccaatt gagctagggc ggcaacccgg ttccaactta ctttatcaaa
      781 aatttgcggt aaacaaaagc cccgcgacgg cccatctccg cttggcaatc acgcaaaaag
      841 ctccttgaca tggagctttt tagattggag aacaaattca aaattcggat tgaaaattat
      901 tcgctggcaa cctgaacctt gttcaccaac attgacaaca gtttttttac ttcctgctgc
      961 gcttcagcct cagtcttgct atttataaat ttgtcccact gggccagcgc ttcgctatca
     1021 tttaggtcgc gcacggcatt ttccaacttc agacgcgtat caaaactaac ctcctgttgg
     1081 gccaacaaaa cgtcatcaat gaacatcttg agaaaaccaa ccaccttggt gttgagcatc
     1141 tccgctcgtt caaacgattg ctggtcagcc aattccgacc gctcgtggaa atatgcccac
     1201 gcccacaaca aattagtgac tatcaaaatg ataactaaaa taaaacttgc tttaattttt
     1261 ctgttgttct ccatattttt tatccctttg ttaataataa ctaattaata attttttgtc
     1321 atcatcagag gcagaggaaa ccggtaatct gtgttgcttt tctggccgca aacgcgactt
     1381 gtctcaggtc tagacccctc gtcacaatgc ttatctttta ttcctttgtc taatcatcgg
     1441 cctcaccacc attccccaga taaccgccag cgcgataacg ataagcgcta ccagctccca
     1501 gttcatccta aagaaccaac cactttttgg tttaagttca atgtttaggt ggataccatc
     1561 cccctctgtc acatcaaaca aatcgctctg ataatcctca aagcccggcg ccactgtcgt
     1621 tatgtaataa gtaccctccg gtaccaagaa cgaataggtg ccacgcggac ctgttacctg
     1681 cgggttctcc tgtaagtatt cttctgccgg ccaaatttga tattcgtctt tttgggaatt
     1741 caaccaccac aaagttacgg tggcttcaga aatacgtgcc tcatacaacc cactcttggt
     1801 gaaaacataa ccttccggat ccaccaccac aatcatatta atttccttaa cccccaaatc
     1861 aggattcaag tagtccatcg ttgtgatcat cttgtatttg ccggccacca ccggtgcttc
     1921 tatgcttgct gaataaatac catcattatc ttcatcgtaa tattcaaatt ccgacaaaac
     1981 gaaggccttt tccgccaact tcgattcctc taacttaatc aattccgaca tcgcaaatct
     2041 ggtcatggct aacaacaaat ttttcggact cctaaacacc gtcttttctt ccaccggttc
     2101 agataacaca atatagcctg ttatttttcg tgtcggcgcg tctggcttaa tcaccaagtc
     2161 cacttttttg ccgacaatag tctgaatttc ctgcaacggc tgacccttgg cgttaaaagt
     2221 tagactggtc tttagatcaa ttctttcctc aagcgcgcgg gcaaaaatag tctcgcttgg
     2281 cattttttcc ttttctgcaa cagacaactc cgccaccggt actcttttca caagtgcaac
     2341 cttattgttt tggagctcag tcttccgcaa accaatcaat tttgaaaagc ccggcatcgc
     2401 caaacgcaat ccttgcaatc gtttgacatc cgacattcga ttcacgccta cttcgcttaa
     2461 cacttggcca acttggggaa atttgtttgt aaacaagact acatcttttg gcaatccttg
     2521 caaagcaaat ctacttactt cgcgcggtgt aatttgccat ttagccttaa gggccagaga
     2581 tatgcttctt accgccgaac taagcgcaaa ccgctccggt tccggactaa gcaaaattga
     2641 agggtacctc agagctaatg gtgtctgtcc ctgccagatg tcacctggcg atatattcga
     2701 ttttttgtta ccaaacaatc tagtttgaat aaaatcaggc gttaggatag ctagtgtttt
     2761 ttttatttta tcaactattt cagtttccca aactttctgc gcaagaggta aaaatgttct
     2821 aggataaaga gaaggggaca tactgcctga cgaaccaaca atttgagatt cagtctccga
     2881 acttgacgag cgccaatccg ttaccacacc acagtgattg cagtcaaaat ttattcggcc
     2941 gtagatatcc ccttccgcca gaccagaaaa acggccactt gtcccatcaa ttgtcacacc
     3001 gtcaaaatca atccaaccgc cattctgact ccaagcctga ccagacaaat taccggcgcc
     3061 atcattcaac accccgccat tttggggtgc caaattgatc cagccgtaaa ttgcgtccca
     3121 ggcataaccg gtgatagcgt tgtctgtcac cgtcaccccg ccatgttcgg tcccaaaatt
     3181 aatccagccg acattttcac cccaagcgta ttggtcgccc tcaattgtcc ctgccatcgc
     3241 caaaaacggc aaaacagaaa tgaaaaaaat taagttctta ataaagattt tcacgcttat
     3301 attgtaaaac tttagaaaaa taagaacaat ctctttttgt ttcggcaaag accagggtct
     3361 aagcatctaa agcttgaagt ttatccgtcg atagacgggt ttttaaaaaa tcgttcctcc
     3421 aattcttctt taacctttac agtctgaaat ccttattttg gtcaatgaac gtcaataaat
     3481 taatagcttt agtccagatt ggtatcaggg gttggattag gctccgaggg gggttcttcg
     3541 tcagccgaag gactggcagt cggttcttct tgactttctt cagccagagg ttcaacctcc
     3601 aaatcactct ccatggtttc tgtgacagtc gtaccagctt catcgaccaa atcttctgca
     3661 ggtggggtgg ttgtactagt ttcatcagcc ggctcttcag taggtggggt ggtcgtacta
     3721 gtttcatctc catcatcaaa agaggtgtct ggtacggcat tatctataat tacttcattt
     3781 gacgctccac cgtcttctct caacgacaag tcttcgcttg tggtaacaga aatttcatca
     3841 cacgcgccaa gagcgctgtg cacttcgccg ttttttatat aaatacaata agtgtcacca
     3901 gtcgctttat caacagtttc atagccttcc aacgagtgga ccttaccggc cttaacgaag
     3961 ccaaagtaac caaccatagc actcactctt tctgccacaa actccttcac caacttaaag
     4021 ctgtttgaaa ttttttcttc aaaataattt ataaagttat cagaaagctc actcgtaatt
     4081 tccgccaggc ggtcttcaat tgtgttggtg gaagaagcaa agataagtcc ggttgttgaa
     4141 gcgcccgtca tagacaggat gattccttgt tgttctttaa tcgcctgcaa aagaaccggt
     4201 gtgaaggcac cgtaagcaac gcctttgatg ccactcgcgc cggtatcaac aaactcgggg
     4261 aaaagggctt ccacttcttg cgcaataaag cctatgtgcg tactactttc cgcgtcttca
     4321 tcagccaccc agtcataacg cacgccacgt aattgcatca ccttatcaag cgccccttca
     4381 agcgtcaaaa tgttcttctt catcctaata tctgaagtac gggcccaatc acccgtagcc
     4441 gccacgtgcc cctcgttaga gtcgtcaata taaacctgaa aagcgtaatc aggcgtggcg
     4501 gtgcccagac cgacgttacc tgtagtgtag tcaacaacca aagagttgct attgacagtc
     4561 aaacctccag caaacgtaga agtagcggta gtacttgtcg cggtaaagta tccagccaaa
     4621 gcctggttgc caaggcttga cgaaccttca acggaaagag aatataatgg tgaggtggtg
     4681 ccaatcccaa cgttgcctcc agagattgtc agaagaccac caaccgaagt aagcgcggcc
     4741 attgaagttg tagcgctatc aaccggcaca aaacccaaag catcttgttt gccgttccag
     4801 aggccctttt caacatccgt caccagtcta tgagttgaat cgtcaagcaa agatgaaagc
     4861 gaagaatagg aagcgcgcaa aatagtggaa gaagcggcgg cgtaaagcgg gtcggtttca
     4921 gtgtaagaat acaaaggaga accaaagtcc gagatttgag atttggtcat tgtcaagttg
     4981 ggaagcaaaa ggattgaagc gactgaagtt gtcccaacat caatcttacc agccagagcg
     5041 gccatgagat cggtttgggc ggaaagcgcg ccggtgattg cgccccaact gggatcagca
     5101 acagaagtgg ttgtggcaat cacgttaccg tcctgatcca cacctaaaat agtacttcca
     5161 agagaagcca aacgaagaat actagagaca acaagtctcg tcgtggtcgc cgctgtcaga
     5221 gaagaaagtt cggtaatgtc atcgtttatt ccagacaccg ccgagggcgt aaccgtgcaa
     5281 tcgggatcgc tcggcccgca atctggatca agtgtttcgc cgggcgaata aaaaggcgcc
     5341 gccgaaacaa ggaaagaaca acacaaaaaa accagcagaa agactacaac attccgaaaa
     5401 aattttccat tttttaactt gtataaaata aagttcaatt ttactttttt gctacaaaaa
     5461 acactctgcc caaaatacag gcttcatgaa taattataca acttatcacc acaaaaaaaa
     5521 gaaaaaacaa gaaagtttgt ggataactaa aaaccctctt tttgaaggtc ttttttatcc
     5581 aatttccaat ctctaacctc taatatctaa actaatctct gagtttcttc ctctttctcc
     5641 cttgccaact aataaacttc taaactattc tctgagccgt tggtcgggat tgaaccgacg
     5701 acctcgtcat taccaatgac gtgctctacc aaagccgacg ccttaggtcg gctccccgac
     5761 ctcgcctctt cggccgatgg cgagcgtcgg ggctgagctc ttaaccaata ctaatgatgc
     5821 catcttttat aatcttttca atataattcc tcctttttaa cctctttaac ttatattcaa
     5881 ttttccttgc ctcctttaaa ttatcaaatt tttggaaaaa taccaactct atagaaccca
     5941 ttcgttttgt ggaaggagta tgcccagaaa ggtgttgttt aatccttcta tccaagttat
     6001 ccgtacttcc tatataatat cctctttctg tttttaaaat ataaactcca caactcattt
     6061 atgtttcctg agccgttggt cgggattgaa ccgacgacct cgtcattacc aatgacgtgc
     6121 tctaccaact gagctacaac ggcttattta atttaattgt aaagaacaaa cttaagctgt
     6181 ggcaagtttc cttcctacca aagccgacgc cttaggtcgg ctccccgacc tcgctcttta
     6241 gcaaactgcg agcgtcgggg ctgagctaca acggcttatt taatttaatt gtaaagaaca
     6301 aacttaagct gtggc
//
LOCUS       DAOOLO010000033         5520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_17704_length_5520_cov_6.195242, whole genome shotgun sequence.
ACCESSION   DAOOLO010000033 DAOOLO010000000
VERSION     DAOOLO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17704_length_5520_cov_6.195242"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="PLH96_02530"
     CDS             complement(<1..1900)
                     /locus_tag="PLH96_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPB60663.1"
                     /translation="MTKHPKVIFMAMAIVGFLGLAVFSAFAEAPSGNPPHCPESNPAC
                     NPPVNVGPASQIKNGALGILGNFSVGFGSSPKTATVIDTLEVKRGIDSTDVISVKPPK
                     ASTVTNASISTSGNLDLVSNNSTAFSNVYIKNSIGGDKGANLVVTKGVVQSIQTASEG
                     SLILGNNNAKVWSPWVSSLHFSVFPDVKGGTGQMAFSFEGNGSTEGRKFMMQKDFFRP
                     MEDNYATLGIPFARWKNVYSVGADVGNITIRGGNPALNKILASDAVGLASWKTAAELG
                     LDSGSGVSNWTLTGTNLTNNNTTGNVGIGITPTARLHVNGSVKVGQKNPLEMGAGLSK
                     ESSAGKIAYQLFSSGLDIVGAGTVNGSRLVKLWDNVEVPSTITTNNLKAVNLCTTAGA
                     CATVQNIINGLSNSLTPGGLGYDIDANGRVDQADQTIVVNCSLGASSCTSLYRLRSDL
                     NRDGRVDAVDVQMITNAALFHKAHWLASGDNIYSSNTGNVGIGISTPVSKLTVNGNIR
                     GATLQSITGSDTGVLTFGSNVNAKVLAPWIDALHFSVSPGKEIGTGSMAFTFGGDGVM
                     TGRKYFLNATAFRPSASGGATLGIPNAPWGTLYSTGADFSGQVTIRGGNPGANKILTS
                     DANGLASWK"
     gene            complement(1928..2278)
                     /locus_tag="PLH96_02535"
     CDS             complement(1928..2278)
                     /locus_tag="PLH96_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60664.1"
                     /translation="MENFNPMEQTNPQEPVQESTPAREGSSIKKIIWIVVAIIIVLLV
                     VLAVIGSRSASQPGELTEQEKQDLIDALNQNPGPELTNEEISTLEAALNEGADKVPEL
                     SEQEKQELLDALNQ"
     gene            2635..3888
                     /gene="nusA"
                     /locus_tag="PLH96_02540"
     CDS             2635..3888
                     /gene="nusA"
                     /locus_tag="PLH96_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPB60665.1"
                     /translation="MMDLKALKVGLDQLEEEKRIPREKILSAIEDALVAAYKKDYGKK
                     GQIIRAKFDLDSGATDFSQIKIVVDETSVRPPEEDGGEETAETLIIEKKEATKEDAGD
                     EGAEILPRFNEEHHIWLEDARKIKKDANIGEELIFPLETKDDYGRIAAQTAKQVIIQR
                     IREAEKETTLDEYREKEGEILSGIVQKVDRGNVFVDLGRTVAIIMREDQIAGEFYRPG
                     ERIRAYLYQVEETPRGINLRLSRSHPQFIARLFETEAPEISSGVVEIKAIAREAGARS
                     KVAVMSHNASIDPVGSCVGQKGSRVSTVINELGGEKIDIIEWSEDEELFISNALSPAK
                     VLKIELDEENHEAQIEVAPDQFSLAVGKGGQNARLAAKLTGWKIDISTPEGTEPEGLK
                     IQSEIANSPEDEEFADGGNVDDGSE"
     gene            3939..4472
                     /locus_tag="PLH96_02545"
     CDS             3939..4472
                     /locus_tag="PLH96_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPB60666.1"
                     /translation="MNLWHEIEPGEAEEMNVIIEIGKGSKNKYEVDKKTGLIKLDRAA
                     HTAQDFPFDYGFVPKSLWHDNDPLDVIVLTTFPLQPGILVEARPVGVMHMIDDGEADD
                     KIIAVPKEDPRWSEVKDLGDVNKHTLKEMEHFYSTYKALQNKEVKVNGFGGAAKARAI
                     FKEGLELYENKFVKQPV"
     gene            4542..4757
                     /locus_tag="PLH96_02550"
     CDS             4542..4757
                     /locus_tag="PLH96_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60667.1"
                     /translation="MQDEETIGPVIGIVLILAIIFFGGLFAFLGYGQQENEPVIEMPI
                     NKGVPEETATASEIIFETGPAEEGIAE"
     gene            4925..>5520
                     /locus_tag="PLH96_02555"
     CDS             4925..>5520
                     /locus_tag="PLH96_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HPB60668.1"
                     /translation="MEIKVKINKTADAEVELEGEIAATDFGSFWPKAIKTLGEKAKID
                     GFRPGHIPEKVLVEQLGENGVLYEMANQALGQAYAQILTENKIDAIGEPQITITKIAK
                     DNPLGFKIKTAVLPKIDLGDYKKIAQKVNGKPLEETVISDEEVNKVIEDLRQQRAAAN
                     QTKHEHKEGEKCDHDEPKLPELNDDFAKSLGQFKNLDD"
BASE COUNT         1705 a         1256 c         1240 g         1319 t
ORIGIN      
        1 ttttccacga agccaagcca ttggcatcgg aagtcaaaat tttgttggcc cccggattgc
       61 cgcccctgat tgtaacctga ccagaaaaat ctgcgcctgt tgaataaagg gttccccagg
      121 gggcgttagg gatacctaag gtggcgcctc ctgaagcgga gggcctaaaa gcggtagcat
      181 ttaagaagta tttacgacca gtcatcacgc catcacctcc aaaagtgaag gccattgagc
      241 cagtccctat ttctttgccc ggggaaacac taaaatgcag agcgtcaatc caaggagcca
      301 agaccttggc attgacgttt gaaccaaaag taagcacacc ggtatcagaa ccagtaattg
      361 actgaagggt cgcgcctctg atattaccgt tgactgtcaa tttagatact ggagtagaaa
      421 tcccaatccc cacattgccg gtgtttgaac tgtatatatt atcaccagaa gctaaccaat
      481 gagctttatg aaacaaggca gcgttagtta tcatttggac atccactgca tcaacgcggc
      541 catcacggtt gaggtcagaa cgtaaacgat aaagagaagt acaagaagat gcacccaaac
      601 tgcaattcac cacaattgtt tggtctgctt ggtcaactcg gccattggca tcaatatcat
      661 agcctagacc gcctggggtc aaactgttgc taaggccgtt tataatattt tgcactgtgg
      721 cgcaagcgcc agcggtggtg caaaggttga ccgcttttaa gttgtttgta gtgattgtgc
      781 taggaacttc cacattatcc caaagtttaa ctaatcgcga cccgttaacg gtgccagcgc
      841 caacaatatc caatccagaa gaaaaaagtt gataggcaat tttgccagcg ctgctttctt
      901 tggacaaacc ggctcccatt tcaagcggat tcttttgtcc caccttaaca ctgccattaa
      961 catggagtct ggctgtcgga gttatcccaa ttcccacatt gccggtggta ttattgttgg
     1021 taaggttagt gccagttaat gtccagttgg aaactccgct gccactgtcc aacccaagtt
     1081 cagccgctgt tttccaggaa gccaagccta ccgcgtcaga agctaaaatt ttgttaagtg
     1141 cgggattgcc accgcgaata gtgatattgc caacatcggc cccgactgaa tagacatttt
     1201 tccagcgggc gaacgggata cccaatgtgg cataattatc ttccattggc cggaaaaagt
     1261 ctttctgcat cataaacttt cggccttcag tcgaaccgtt tccctcaaaa gaaaaggcca
     1321 tctgaccggt tcccccctta acatccggaa aaacactgaa atgcagagag ctcacccaag
     1381 gcgaccaaac tttggcgttg ttattaccca aaatcaaact gccctcgctc gccgtttgaa
     1441 tagactgaac cacgcccttg gttacgacga ggttggcgcc cttatcacca ccgatggaat
     1501 ttttgatata aacattagag aaagccgtgc tgttgttgct gaccaaatcc aaattgccgg
     1561 aagttgaaat gctggcattg gtaacggtag aagccttggg gggcttaacg gaaataacat
     1621 ctgtagaatc aattcccctt tttacttcca aagtatcaat gacggtcgct gttttggggc
     1681 ttgaaccaaa gccaaccgaa aaattcccca aaatacccag cgcgccattc tttatctgag
     1741 aagccgggcc gacattgacc ggtggattac aagccgggtt gctctccgga cagtgtggcg
     1801 gattaccgga gggcgcttcg gcgaaggcag aaaacacggc caggccgaga aaccccacga
     1861 tagccatagc cataaaaata acttttggat gctttgtcat agtttatgat taattattgt
     1921 tgttaaccta ttggtttaaa gcgtcgagca attcttgttt ttcttgttca gacaactccg
     1981 gcactttatc ggccccttca ttcaaggccg cttctaaagt tgagatttcc tcgttagtta
     2041 attccggacc gggattttga tttaaggcat caatcaaatc ttgtttttct tgttcagtta
     2101 attcgccagg ttgactcgct gagcgactgc cgataacggc caaaaccacc agcaacacaa
     2161 taataatggc gacaacaatc cagataatct ttttgattga gctgccttcc cgagctggcg
     2221 ttgattcttg gaccggctct tgggggttcg tttgctccat tgggttaaaa ttttccatag
     2281 aaatattatt tgttaaatta ttaataagac aattttgacg taatcatcaa aacttaggga
     2341 ccctcccttc tagctatcat tatattacaa accaacccgc cctggtagcc gatgtcaacc
     2401 aaaactgttg ataactagtc ttgcacagat aaaacgcaga cgttcacaga aataaactta
     2461 ggttttatca gcatcctata tgaatttttt aacatctgcc ctttgacctt ttgtttacgt
     2521 agtgatacaa taaaaagagt ccgagcttga cagagttgcg gacttttttg ttaaaaaaag
     2581 aaatgagggc ccaaaaccct tgattaatat tattaaccct aaatcattat aattatgatg
     2641 gatttaaaag ctctcaaggt cggtcttgac caactggaag aggaaaaacg catcccccga
     2701 gaaaagattt taagcgcgat tgaagacgcc ttggtcgccg cttacaaaaa agattatggc
     2761 aaaaagggcc agattatcag ggccaaattt gatttggata gcggcgcgac cgatttttcc
     2821 caaatcaaaa tagtcgtgga tgaaacaagc gttcggccgc cagaagagga tggtggggaa
     2881 gagacagcgg aaactctaat catagagaaa aaagaggcga caaaagaaga cgctggggac
     2941 gaaggagcag aaattttgcc tcgtttcaac gaagaacacc atatctggtt ggaagacgcg
     3001 cgcaaaatca aaaaagacgc caatattggc gaagaactaa tcttccccct agaaaccaaa
     3061 gatgattatg gccgaattgc cgcccaaacc gccaaacaag tcattatcca acgcattcgc
     3121 gaagctgaaa aagaaaccac ccttgacgaa tatcgagaaa aagagggaga aatcttaagc
     3181 ggcatcgtcc aaaaagtaga ccggggtaat gttttcgtgg acttgggccg cacagtggcc
     3241 attattatgc gtgaagacca aattgccggc gaattttatc gtcccggcga acgaattcgc
     3301 gcgtatttgt atcaagtgga agaaacgccg cgcggcatca acttgcgttt atcccgttct
     3361 catccacaat tcattgccag attatttgaa accgaagcgc cagaaatctc cagcggcgta
     3421 gtagaaatca aggccattgc ccgcgaagcc ggcgctcgct ccaaagttgc cgtaatgtca
     3481 cacaacgcga gtattgaccc tgttggttcc tgcgtcggtc aaaaaggttc ccgcgtttct
     3541 acggttatca acgagctagg aggagaaaaa attgatataa ttgaatggtc cgaagacgaa
     3601 gagttgttta ttagcaacgc tctctcaccg gccaaagtcc taaaaattga actggacgaa
     3661 gaaaaccacg aggcccaaat agaagtggcg cccgaccaat tttctttagc cgtgggcaaa
     3721 ggtggccaaa acgcccgctt ggctgccaag ttgaccggtt ggaaaattga catcagcacg
     3781 cctgaaggaa ccgaaccaga gggtctaaag attcaaagcg aaattgctaa ttctcctgaa
     3841 gatgaagaat tcgcggacgg cggcaatgta gacgatggta gcgaataaat gcgatattaa
     3901 caaacgaatt tacaaataat taaactcaaa caaaatatat gaatctatgg catgagattg
     3961 agccgggaga agccgaggag atgaacgtca taatcgaaat cggcaaaggc tcaaaaaata
     4021 aatatgaggt agataaaaaa accggtttaa tcaaacttga tcgcgccgct cacaccgcgc
     4081 aagattttcc gtttgactac ggttttgtgc caaagtcgct ttggcacgac aatgatcctt
     4141 tggacgtaat tgtcttgacc accttcccgc tccaacccgg catcttggtg gaagccaggc
     4201 cggttggggt aatgcacatg atagacgatg gcgaagccga cgacaaaatt atcgctgttc
     4261 ccaaagaaga cccgcgctgg agcgaggtca aagaccttgg cgatgtcaat aaacacaccc
     4321 taaaagaaat ggaacacttt tactccacct acaaagcgct tcaaaacaaa gaggtaaaag
     4381 ttaatgggtt tggaggagcg gccaaagcgc gagccatttt taaagaaggg cttgaacttt
     4441 acgaaaacaa gttcgtcaaa caacccgtct aacggccgat tcccctttcg cccaaaattg
     4501 ttataataaa tttagcttat caatctaact ctctctaaaa tatgcaagac gaagaaacaa
     4561 ttggaccagt tattggcatc gtgctgatat tggccattat cttctttggc ggtttgtttg
     4621 cctttttggg ctatggccaa caagaaaacg agccagtcat agaaatgcca attaacaaag
     4681 gggttccgga agagacagcc acggctagtg aaattatttt tgaaaccgga ccggctgaag
     4741 agggaatcgc ggaatagaaa acccctctca cccggaatgc ttttgtttcg tgtcaattgc
     4801 tgaaggtgaa aaatacttca gcgcttgacg gtcagacgga ttttgccgtc caaatattta
     4861 gagtgagaca aaaaacaaca agaatgttcc aggcgagagg aaactaacaa caaaaataga
     4921 aaatatggaa ataaaagtaa aaataaacaa aacagcagat gccgaagtag agcttgaggg
     4981 tgaaatcgcc gcaaccgatt ttggctcgtt ttggccgaaa gctattaaaa ccttgggtga
     5041 aaaagcaaaa atagacggct tccgcccagg ccatatcccc gaaaaagttc tagtggagca
     5101 gctgggagaa aacggtgttt tatacgaaat ggccaaccaa gccttgggac aagcttatgc
     5161 tcaaatcctg acagaaaaca aaattgacgc tattggcgaa ccacaaatta ctatcaccaa
     5221 aatcgctaaa gacaatccgc ttggtttcaa aatcaaaact gccgtcttgc ccaagattga
     5281 tttaggcgat tacaaaaaaa tagcccaaaa agttaatgga aaacctttgg aagaaactgt
     5341 catctccgat gaagaggtca acaaagtgat tgaagactta cgccaacaac gcgcggctgc
     5401 caaccaaacc aagcatgaac acaaagaggg cgagaagtgc gaccatgacg agccgaagtt
     5461 gcccgagtta aatgatgact ttgccaaaag cttggggcaa ttcaaaaacc tagatgattt
//
LOCUS       DAOOLO010000034         5092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_19733_length_5092_cov_5.624181, whole genome shotgun sequence.
ACCESSION   DAOOLO010000034 DAOOLO010000000
VERSION     DAOOLO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19733_length_5092_cov_5.624181"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1060
                     /locus_tag="PLH96_02560"
     CDS             143..1060
                     /locus_tag="PLH96_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPB60669.1"
                     /translation="MRKTIIFVAILGIALVGFGQAQATTTATTTISSLLEQLSALQTQ
                     LQELRSQQQTIIADLTRNLSLGASGDDVKALQEFLASQPGVYPEKLATGYFGPLTLNA
                     VKKFQKINGLEAVGNVGPLTRNLIKNFKSKKGVSILENSPTTPIGTTTPISLGSTITI
                     CHYPAGNPANKQTITIGAAAWKAHQKHGDTMGPCDGTSSNDDEDDNDDDLKDVTAPVI
                     SDIEKEIASTTASISWKTNEAATGKFYYGTTDPLALASSTLINLASNTSHDVNLSDLA
                     TSTTYNFLIEATDAAGNKASISQSFNTTE"
     gene            complement(1239..1496)
                     /locus_tag="PLH96_02565"
     CDS             complement(1239..1496)
                     /locus_tag="PLH96_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60670.1"
                     /translation="MELDCVWQGERVKGEKVMTNTVVVRLGQAPTVTEVTQMAFQKDA
                     FREQEGDFIYHVRLGGSPARWYYSEELEEALAKHLARYCAQ"
     gene            complement(1703..2011)
                     /locus_tag="PLH96_02570"
     CDS             complement(1703..2011)
                     /locus_tag="PLH96_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60671.1"
                     /translation="MGAMSFESTNNEISPEVLGNKFNSEDFSDFVADLRQRIKANPSI
                     RFTIVADWVSAEKARSAKALLDQYASRLNIDSITIRATRQQYNEEINVFESGVKWQEL
                     "
     gene            complement(2172..3113)
                     /locus_tag="PLH96_02575"
     CDS             complement(2172..3113)
                     /locus_tag="PLH96_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB60672.1"
                     /translation="MKILVTGGAGFVGSHLCQKLLAQGNEVICLDNFQTSSKENIQNL
                     LTNPKFTFILHDVTVPLEIKVDQIYNLACPASPIHYQLDPVKTVKTSVLGAINMLELA
                     KNNQAQILQASTSEVYGDPHIHPQTETYWGNVNCLGLRACYDEGKRCAETLFFDYHRQ
                     FKVAIRVIRIFNTYGPQMAENDGRVVSNFIIQALKNEPLTIFGQGNQTRSFQYIDDLI
                     AGMTKMMDNDQDFIGPVNLGNPTEFTIKELAELVLKLIPQSASQIVYQPLPEDDPRQR
                     RPDIALAQKKLNWVPQIKLEDGLKATIEYFKEKLKKH"
     gene            complement(3258..3530)
                     /locus_tag="PLH96_02580"
     CDS             complement(3258..3530)
                     /locus_tag="PLH96_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60673.1"
                     /translation="MEEEIQKLKQELDTIKERNNRVEGDKAWETSYFRVILISLITYV
                     LASITFYLIGNPNYFLNALIPTLGYFLSTQSLPFIKKWWINKSSRK"
     gene            complement(3995..4951)
                     /locus_tag="PLH96_02585"
     CDS             complement(3995..4951)
                     /locus_tag="PLH96_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60674.1"
                     /translation="MKKTIIILIGILIVGVAITLIIITSFEEVTDEPIVEPIDKETLE
                     EKEELESVKSENQQINEKVSTQTRVNETDLEETESKAPSYSDNSYHPADINKDYKISR
                     EEIEIAEGLFQQGEITKTQLSQAVEFFNIPNGYRIQKELDNGNFPTNVNFRNCDYSGF
                     FDEKGGIFRCQNGGGIEVKIDNSKAMGNFKIGLEITVPEEKEINERQDSSIPYLGAIK
                     IDLKEIGQSPQDYSGPAPVFTVNVPLKSKLPPGTRLEARLKGDYIGLGIQEWRLQGEG
                     LAAVVSDDGLTASFESKELPGLFIVSRFVLPIINYDEILNQN"
BASE COUNT         1468 a         1108 c          949 g         1567 t
ORIGIN      
        1 attaagttaa ttttaacata ggcacaattt cttcatatgg tggataattc ccagcttgac
       61 tttattaaaa tatgttataa ttaaattagt cattatcaat tatgacttaa ttattagagt
      121 attattagtt aaaagtaaat ttatgagaaa aactataatt tttgtggcaa ttttaggaat
      181 cgccctggtt ggttttggac aggcccaagc aacaacaacc gccaccacaa caatcagcag
      241 cctgcttgaa cagttgtcgg cgttacaaac acaactccaa gagttaaggt cgcaacaaca
      301 gacgattatc gccgatttga ctcgcaactt gtctttgggc gcaagcggag atgatgttaa
      361 agctttgcaa gaatttcttg cttcccaacc aggcgtttac ccagaaaaac tggctactgg
      421 ctattttggt cctctaacct taaacgcggt taaaaaattc caaaaaatca atggccttga
      481 agctgtcggt aacgtaggtc ctcttactcg taaccttata aaaaacttta agtccaaaaa
      541 aggagtttca attcttgaaa attcccccac cactccgatt ggcacaacaa cgccaatctc
      601 attaggttca acgataacta tttgtcatta cccggccgga aacccagcca acaagcaaac
      661 gattacaatc ggcgctgctg cttggaaagc ccaccaaaaa catggcgaca ccatggggcc
      721 ttgtgatggg accagttcaa atgacgatga agacgataat gatgatgatt tgaaagatgt
      781 cacagccccg gttattagcg acatagaaaa ggaaattgcc agcaccacgg cttctatttc
      841 ttggaaaacc aacgaggccg ccactggcaa gttttactat ggaacaacag accccctggc
      901 gttggccagt tcaactttaa ttaatcttgc ctccaacact tctcacgatg taaatttgtc
      961 tgacctggca actagcacaa cttacaactt tttgattgag gcgacagacg ccgccggcaa
     1021 caaggctagc ataagtcaat cattcaacac aaccgaataa caaggacaag aaatagctca
     1081 tctaaaaacc ccgcctaagc ggggttttta aattctttta ttattacaga cttatgaaaa
     1141 ttaattattg ttcatagcta caaaagataa gcaaacacaa gcagacccca aaaagaaaac
     1201 tcaccgaagc gagtttcctg ccgccagaag cgacaaactt attgcgcgca ataccgcgcg
     1261 agatgtttag ccaaggcttc ctccagttct tccgaataat accaccgtgc cggtgacccc
     1321 cccaaacgaa catggtaaat gaagtccccc tcttgttccc gaaaggcatc tttctgaaat
     1381 gccatttggg ttacttccgt gactgttggg gcctgcccca aacgcaccac caccgtattt
     1441 gtcatgacct tctccccctt tactctttca ccttgccaaa cacaatccaa ttccattaaa
     1501 acaaagcccc attttatgtc aaggattgtt atcaactctt gcctccaaaa tttttaacaa
     1561 tttatcaact atgattgcat ggcaaagaac gatttttcca tctagagttt ttatcctccg
     1621 acatttcacg acaaaattga taggctcagg tttttattga acgatgtcct ccttctcatt
     1681 aatgaatcat tttatttttc ttttaaagtt cttgccattt tactccggac tcaaagacat
     1741 ttatctcttc gttatactgc tggcgcgtgg cccgaatagt aattgaatca atgttcaatc
     1801 tggaggcata ttggtccagg agcgctttgg cagaccgggc tttttccgcg cttacccaat
     1861 cggcgacaat ggtaaaccga attgacggat tggcttttat tctttgacgc aggtcagcga
     1921 caaaatcgct aaaatcttca gaattaaatt tgttgcccaa cacctccgga cttatttcgt
     1981 tgtttgttga ctcaaatgac attgccccca taaattaaga aatgaattat tttgtttaat
     2041 gttttggcaa aagcttgctt gacgatttcc tggcttaatt ttatcaaaaa caaagaaaga
     2101 accaagagag gctcctctgc cctctgtgac ttttgaccga caatcaaaaa tgaaccaagt
     2161 cgctttaaga attaatgttt ttttaatttc tccttaaaat attcaatggt cgccttgagc
     2221 ccgtcttcca acttaatttg cggcacccaa tttaattttt tttgggccaa agcaatgtct
     2281 ggccgccgtt gccgagggtc gtcttcgggt aagggctgat agacaatctg gctagcgctt
     2341 tggggaatta atttaaggac taattccgct aattctttaa tggtaaattc ggttggattg
     2401 ccaagattga ccggaccgat aaaatcttgg tcgttatcca tcattttagt cattccggca
     2461 attaggtcat caatgtattg gaaagacctc gtttgattgc cctggccaaa aatagtcagg
     2521 ggttcgtttt taagggcctg aatgataaaa ttagatacta cccgaccgtc attttccgcc
     2581 atttggggac cataggtatt aaaaatcctg atgacccgga tagcgacttt aaactggcga
     2641 tgataatcaa aaaataaagt ctcggcgcaa cgcttgccct cgtcgtaaca ggcccgcaag
     2701 ccaaggcagt tgacattgcc ccaataagtc tcggtttggg ggtggatatg ggggtcgccg
     2761 taaacttctg acgtggaggc ctgcagaatt tgagcctgat tgtttttggc taattccagc
     2821 atgttgatgg ctcccaaaac cgaggttttg acagttttga ccggatccaa ttgatagtga
     2881 atcggcgaag ccggacaagc taaattgtaa atttggtcaa ctttaatttc cagaggaaca
     2941 gtaacatcat gaagaataaa ggtaaacttc gggttggtaa gtagattttg gatattttct
     3001 ttgctggaag tttgaaaatt atccaagcag atcacttcat tcccctgcgc caataatttt
     3061 tggcagagat gggagccgac aaaaccggcg ccgccggtaa ctaaaatttt cataagtggt
     3121 tttaaaaatg aaatttattt gtccgagcag tataatttgt attttttcat atccggtgag
     3181 gaatgtaaag cgggtggaaa taaatgctga acaaaaccaa gaggagtccc tccagggcgg
     3241 tttattagga gctttattta tttacggctg cttttattta tccaccactt tttgataaac
     3301 ggcaatgatt gggttgagag aaaataacct aaagttggga taagggcatt taaaaaataa
     3361 ttagggttgc cgattaaata aaaagtaatt gaagccaaaa cataagttat caagctgatt
     3421 aaaataactc tgaaataact agtttcccaa gctttgtcac cttcaaccct gttatttctt
     3481 tctttgatag tatctaactc ttgttttagt ttttgaattt cttcttccat tcttaaatca
     3541 taccaaaatt ccccgatttg aaaaacaatt agaaaaaatt ttgtataaaa ataaaggaaa
     3601 accgctcgca cttgcgagcg gtttttggca aggtggcgat tgctcagagt tctcccggtg
     3661 ttttgttcaa ccggaccgcg tctgacaatc cgcaaagacc ccgagcgcct tcaccactca
     3721 ccgagcgacc tcggttgtca gtgtcggtca ccgtgatccc gccatcagca cgttcgtcaa
     3781 cggtgcgagt cgaaccatcg gaattgtgcg tagtgcaccg attctggctg ccgtcaccaa
     3841 tcttcgacca ttccttaggc ataaaatcac ctcctttcta ttcagaaatg catcgcaaaa
     3901 ctatagattt gtctatctga tccacaccct aaacaaaatt ttctctttcg tataaaactc
     3961 caactccctt aaaagggtaa agaatttatg ccatctaatt ctggtttaaa atttcatcat
     4021 agtttattat tggcagcaca aagcgactga caataaataa accggggagt tctttcgatt
     4081 caaaggaagc agtcagtcca tcatcgctca ccacggccgc gagcccttcg ccttgtaatc
     4141 tccattcttg tatccccaat ccaatataat cacctttcaa acgcgcttcc aatcttgtcc
     4201 ctggtggaag ttttgatttt aacggtacat taacagtaaa aacaggtgcg ggcccggagt
     4261 agtcttgagg gctttggcct atttctttga gatcaatttt tattgctcct aaatatggta
     4321 tagatgagtc ctgtctttca tttatctcct tttcttcggg aacggttatc tcgagaccaa
     4381 ttttaaaatt acccatggcc tttgagttat cgatttttac ttcgatgcct cctccgttct
     4441 gacaacgaaa aatcccccct ttttcgtcaa aaaatccgct gtagtcacag ttacgaaaat
     4501 ttacgtttgt cgggaagttt ccattatcaa gttctttctg aattctgtag ccatttggaa
     4561 tgttgaaaaa ttcaacggct tgggaaagct gggttttggt tatttcccct tgttggaaaa
     4621 gcccttcggc aatttcaatt tcttctcgac taattttata atccttattt atgtcagccg
     4681 gatgatatga gttatctgaa tagcttggag ctttactctc tgtctcctca agatccgtct
     4741 cgttaactct tgtttgggtg cttacttttt cgtttatttg ttgattctcg gattttacgc
     4801 tttctaattc ttctttttct tctaatgttt ccttgtctat tggttctact attggctcat
     4861 cggtaacttc ttcaaaagaa gtgattataa ttaaagttat agccactcct acaattaata
     4921 ttccgattaa aattataata gtttttttca tattttttat tttattaatc caatcaatct
     4981 ttatttaatt tagcatatca caaaataaaa acccgaggaa tatctcgaga attgttaata
     5041 gaaaaaattt caaaattttg cgaagatttt aattttttgc tccgcgggca gg
//
LOCUS       DAOOLO010000035         4891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_20811_length_4891_cov_5.256410, whole genome shotgun sequence.
ACCESSION   DAOOLO010000035 DAOOLO010000000
VERSION     DAOOLO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20811_length_4891_cov_5.256410"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLH96_02590"
     CDS             <1..349
                     /locus_tag="PLH96_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60675.1"
                     /translation="FIQIGLKPDSKDLEKKIKDRLLARMRLGLVAEVKKLHRQGLSWR
                     RMEELGLEYRYLALLEQKKTSREQMIKELEKEIVRYAKRQITWFKRDKRISWFNPTDK
                     KLLEKVLKLINEN"
     gene            complement(346..699)
                     /locus_tag="PLH96_02595"
     CDS             complement(346..699)
                     /locus_tag="PLH96_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60676.1"
                     /translation="MTTTVREKAKKLGGLLAASPIAHDLKMAILENLAKMTERHIDAL
                     VVCLENEAKGIEILIKEADDFAEAQDADWDKVAEEQVDLAAKIVSEEVRQLEKEDQIS
                     ALRQNIINQTEGPSA"
     gene            complement(718..3117)
                     /locus_tag="PLH96_02600"
     CDS             complement(718..3117)
                     /locus_tag="PLH96_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60677.1"
                     /translation="MGELGDDDEDLESLSGSVPGQEGGVGREAEVPLPDPDLLIMNEN
                     TNIEELEELFEKNNKLHGLEQFTDQADVGVGASDNEEVIYPKGSNNPGESEDSPTPLN
                     TEDPKNKELTSVGNSENPGQQLEFTDDAQERIAQNKARIEFLNKAEQEGQLSEIYGYN
                     SNFQELAEREITSLKAEIRELENNSRAFKNESSSPVPGIPRYALERKLKKEELESNSE
                     QAEEVPETTDETRETETEKTSDDDKQPPKSQEVDVDFGEDVDVTQEGQEGKRRLERRE
                     QREDFRNPPIENWRDLYQAIWEVGRIESSKQVFEPEDNIELVVRVRLGENPILLTRTK
                     GLRRAVLLLLASEAGEEAVQQGQQEQGEGPEEPGVEETEMEATNPDAVPVPVPETLPE
                     AILEGQPETVNENRENAIDAEFRVIESENGEGHEEGGEEETESGVGEDVGNAAREAFE
                     NANGQNSLEREREKRGRHWWNWLKERAKGLFTFGFWEVHQAEKVRMAGKKAEKEILQG
                     AGYMKKEGVMEETDIELIKTRLRESGNDVDNMSEEQLRETYQWLSEAVTERKVIANQQ
                     IEDEVVERSIAEIEKRMSGYRKYDGEKIEMTPDKKLSPEKRAEIEQKIRGALAAVRQG
                     QVERDMIKFSKIVRSSLDKSWYKRYVASGIEAALGFAGIKWLVVPMVDSYTKAKATEA
                     ALQTRRVISAVENAVSSGSPIGDVGSTAVEATETTRGLQDNLWNMAKGLLKEKGLGNP
                     TDGQIMEVTKQLAADNSVGVNKWGMAGNILDTKMPVGMAIKVGGAALKAASLAKIAVG
                     L"
     gene            complement(3281..3351)
                     /locus_tag="PLH96_02605"
     tRNA            complement(3281..3351)
                     /locus_tag="PLH96_02605"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3317..3319),aa:Trp,seq:cca)
     gene            complement(3442..3600)
                     /gene="rpmG"
                     /locus_tag="PLH96_02610"
     CDS             complement(3442..3600)
                     /gene="rpmG"
                     /locus_tag="PLH96_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPB60678.1"
                     /translation="MSQDFLIRLSCKSCKRINYWSRKNKKKVERKIDLNKYCKWCKKH
                     TQHVETKK"
     gene            complement(3801..4760)
                     /locus_tag="PLH96_02615"
     CDS             complement(3801..4760)
                     /locus_tag="PLH96_02615"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HPB60679.1"
                     /translation="MAKIKNKYKHLSLAERYVIEKLLLASITIRGIALILGRSPSTIS
                     NEIKRNSVKGQYIAKKAAHKSYVKRKYSKTQSLKVVMDMFLYRFVEEKLNKKWSPEQM
                     SGYLKRQNITVSTKAIYKFIGSRGWEYKLFWSRHNKKSGRKQYRYGNPIDGRKYIELR
                     PEVTEPGHYEMDFIVSKQSPRVLLVIVDRFLKKSWLIKLPNRKHATICDALRQTFSGQ
                     PLLSLTTDNDIAFLKWQNIEELLNVSIYFCHPYHSWEKGLVENTNRWIRCFIAKRRDI
                     GSVTQEELAEIHTFLNDRPRRCLGYFTANELELNYQSVFGLRG"
BASE COUNT         1210 a         1211 c          877 g         1593 t
ORIGIN      
        1 ttttattcaa attggcctca aaccagattc taaagatttg gaaaagaaga taaaagaccg
       61 gctcttggcc cgaatgagat taggtctggt tgccgaagtc aagaaactcc accgacaagg
      121 cttgtcttgg cgacgaatgg aggaactggg tttggaatat cgatacctag ctcttttgga
      181 acaaaagaaa actagccggg aacaaatgat aaaggaatta gaaaaagaaa tcgtccgcta
      241 cgccaaaaga caaataactt ggttcaaaag agataaacga ataagttggt ttaacccaac
      301 tgataaaaaa cttttggaaa aagtattaaa actgattaac gaaaattaag cggaagggcc
      361 ttcagtctgg tttatgatat tttgccgcaa ggcacttatt tgatcttctt tttccaactg
      421 tctgacttcc tccgaaacaa tcttggccgc caagtcaact tgttcttcag ccaccttgtc
      481 ccaatcagcg tcctgcgctt cggcaaaatc atcagcctct tttatcagaa tttcaatgcc
      541 ttttgcctcg ttctccaagc aaacaacgag cgcgtcaata tgtctttccg tcatcttggc
      601 caagttttct aaaatcgcca tttttaggtc atgagcgatc ggcgaagcag ccaagagccc
      661 gcctaatttt tttgcttttt cacgaactgt cgtcgtcata aatttaaaac tttttaatta
      721 taaaccgacg gctatttttg ccaaactagc cgccttcaag gccgcgccac caaccttgat
      781 tgccatgccg actggcattt tagtatcaag aatattgccg gccattcccc acttgttcac
      841 tcccacgctg ttatcagccg ccaactgttt ggttacttcc attatttggc catctgttgg
      901 attaccaagt cccttttctt ttagtaaacc cttggccata ttccataaat tatcctgtag
      961 acccctggtt gtctcggtcg cttcaacggc agtcgaacca acatccccaa ttggagaacc
     1021 tgatgaaaca gcgttttcaa cagccgagat taccctccga gtttgcaaag ccgcttcggt
     1081 tgccttggcc ttggtataag aatcaaccat cggcactacc aaccacttta tccccgcaaa
     1141 accaagtgcc gcctcaatac cgctagccac gtaacgcttg taccaactct tgtccagact
     1201 acttctcact attttggaaa atttaatcat atctctctcc acctgtcctt gtctgaccgc
     1261 ggccaaggcg ccgcgaattt tttgttcaat ctcggcccgc ttttccggcg agagtttttt
     1321 gtccggggtc atttctatct tttcgccgtc atattttcgg taaccggaca ttctcttttc
     1381 tatttcggca atagatcgtt caacaacttc atcttcaatt tgttgattgg caataacctt
     1441 tctttctgta actgcttctg ataaccactg ataagtttcg cgcaactgtt cttcgctcat
     1501 attatcgacg tcgttacctg attcccttag tctggttttt atgagctcta tatctgtttc
     1561 ctccatcacc ccttcttttt tcatataacc agctccttgc aagatttcct tttctgcttt
     1621 tttgcccgcc attctcactt tttctgcttg gtggacctcc caaaaaccaa atgtgaaaag
     1681 gcccttagct cgttccttga gccaattcca ccaatgacgg cctctttttt ctcgctctcg
     1741 ttccaaggaa ttttgaccat tggcgttttc aaacgcttcc ctggccgcgt tgcccacgtc
     1801 ttcgccaaca ccagattcgg tttcttcttc gcctccttct tcatgtcctt cgccattttc
     1861 actttctatt actctaaact cggcgtcaat cgcgttttcc ctattttcgt ttactgtctc
     1921 cggctggcct tccagaatcg cttccgggag agtctcagga acagggacag gaacagcgtc
     1981 tggattagtc gcctccattt ctgtttcttc cacccctggc tcttctggcc cttctccttg
     2041 ctcttgttgc ccctgttgga cagcctcctc gccagcctcg ctagccaaaa gtaacagcac
     2101 cgcccgacgc aagcctttgg tcctagtcaa taatattgga ttctcaccta gccttactct
     2161 aactaccaat tcaatattat cctcgggttc aaaaacttgt tttgaactct ctattcttcc
     2221 tacctcccaa atagcttgat acaagtctct ccaattttca attggtgggt ttcgaaagtc
     2281 ttcccgttgt tctcgtcttt ccagtcttct cttcccttcc tgaccttcct gggtaacgtc
     2341 aacgtcttct ccaaagtcaa cgtcaacctc ctgagattta ggcggttgct tgtcgtcatc
     2401 gctcgttttt tcggtttctg tctcccttgt ttcatctgtt gtttctggga cttcctctgc
     2461 ttgctccgaa ttactttcca actcttcttt tttcaatttc ctttcaagag cataccttgg
     2521 gattccaggg acaggagacg aggattcatt tttaaaagcc ctggaattat tttccaactc
     2581 tcttatctcg gccttcaatg aagtaatctc tcgctcggct aattcctgaa aattactatt
     2641 gtatccgtaa atttcagaca attgtccttc ttgctcggct ttatttaaaa actcaatcct
     2701 ggctttgttc tgtgctattc tctcttgtgc gtcgtctgta aattccaatt gctgacctgg
     2761 attttcagag tttccaaccg aagttaattc tttgtttttt gggtcttctg tatttaaagg
     2821 agtaggtgaa tcttcagatt cgccaggatt gttactcccc tttggataaa tcacttcctc
     2881 gttatccgat gcccctactc cgacatcggc ttgatcggta aactgttcga gcccgtgaag
     2941 tttattgttt ttctcaaaga gttcttctaa ttcttcgatg ttggtatttt cattcattat
     3001 caacaaatca ggatcgggga gaggtacttc cgcctctctc ccgacgccgc cctcttgtcc
     3061 gggaaccgac ccactcaagc tttccaagtc ttcatcatca tcccccaact ctcccaacgg
     3121 aagcaggggg ggggatttga gccagttctt cttctgagta ttcttttttg ggaatttcca
     3181 tactagattt ttatggttca attatactct tttaaagaaa ccatttcacc tgcccctgtg
     3241 gataaaaaac aaaactagag ttatctagtt atctaaaggg cgggagctgg aggaatcgaa
     3301 cccccaacga cggttttgga gaccgtagtt ataccattta actaagctcc cgaagtttga
     3361 ccaaaacaac cagaaactag gtggcatcat aacatagaaa cttcatttaa aaaagtaaaa
     3421 gaaaagccct caccgagagg actatttctt ggtctccacg tgttgcgtat gcttcttgca
     3481 ccatttgcaa tatttattca aatcaatctt ccgctccact tttttcttat tcttgcggct
     3541 ccaatagttg attcgtttgc aagacttgca agacagtctg attaaaaaat cttgtgacat
     3601 aaataaatta atctgcctca gggacagtgt tttttattat agcccaagtt gttgaaataa
     3661 gcaaattttt ggttgagccc tttcaattct tattcttgtt tcattctaaa accagaaaca
     3721 aggacttaga tataataaaa aaacaaaatt gacttttaca cttatttgtg ttaagatgaa
     3781 aagagtgggg ggctcctctc ctaacccctc aagccgaaca cagattgata atttaattct
     3841 aactcattgg cggtgaaata accaaggcat cgtctgggtc ggtcattcaa gaaggtatgg
     3901 atttctgcca attcttcttg agtcactgaa ccgatatctc ttcttttggc gataaaacat
     3961 ctaatccacc gattagtgtt ttccaccagt cctttctccc aagagtgata tgggtgacag
     4021 aaatatatgc taacatttaa gagttcctca atattttgcc atttgaggaa cgctatatcg
     4081 ttgtcagtgg ttaggcttaa aagaggttga ccactgaaag tttgccgaag cgcgtcacaa
     4141 attgtggcgt gctttcggtt tggcaactta atcaaccaac ttttcttcaa gaagcgatcc
     4201 acgatcacca ataaaacccg ggggctttgt ttagaaacga taaagtccat ttcataatga
     4261 ccgggttctg tcacttctgg tcgcaattct atatactttc gaccatcgat tgggttgccg
     4321 taacggtact gtttgcgacc tgatttcttg ttgtgtcggc tccaaaacaa cttgtattcc
     4381 caaccacgac tgccaatgaa cttatagatg gctttggttg aaactgtgat attttgtctt
     4441 tttaaataac cgctcatttg ttccggagac cattttttat tcaatttctc ctctacaaaa
     4501 cgatagagaa acatatccat caccactttc agagattggg ttttggaata tttgcgcttc
     4561 acatatgact tgtgagcggc tttctttgct atatactggc ccttgaccga attcctttta
     4621 atttcattgc taattgtact tggactgcgt ccaagtatga gggcaatacc acgaatagtg
     4681 atagatgcca aaagcagttt ttcaatgacg tatcgttccg ccaaagataa atgcttatat
     4741 ttgtttttaa tttttgccat acacctattt tactaggtgt tcgacttcaa aggttaactg
     4801 aagctaaagt accgtaaacg gaccccagac ccccctctac gagacgtggg gtttttagtc
     4861 cttgcattaa aatatttatc tctctacatc a
//
LOCUS       DAOOLO010000036         4692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_21992_length_4692_cov_5.449429, whole genome shotgun sequence.
ACCESSION   DAOOLO010000036 DAOOLO010000000
VERSION     DAOOLO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21992_length_4692_cov_5.449429"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PLH96_02620"
     CDS             complement(<1..1083)
                     /locus_tag="PLH96_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPB60680.1"
                     /translation="MIEKMTDSILFIVLGLILGAGGVFLAWLLFGRRGLKDDQNGANN
                     ASAESLLLLQNQLNTLIKTVDDKLGESARHMQNSIQTQFSESSKIIRDVTERLTKLDE
                     TNRQVVGFADQLQSLQDILKNPKQRGILGEYYLETLLKNVMPPGSYQMQYPFADGTIV
                     DAAVFVKDKIIPIDSKFSLENYNRLAEEKNETERDRLEKVFVNDLKLRIQETAKYVQP
                     DKGTLEFAFMFIPHEAIYYDLIVNKIGALKDENTENLIQRAAAKYHVIIVSPTSFLAY
                     LQTVLQGLKALQIEESAKEIKKRVEELGKHIGAYENYLAKLGGSLGTTVNHFNSAYRE
                     LKKVDKDVLRIAGESNGFEPTLVEKPV"
     gene            complement(1136..1357)
                     /locus_tag="PLH96_02625"
     CDS             complement(1136..1357)
                     /locus_tag="PLH96_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HPB60681.1"
                     /translation="MGKFKALDWLAIVLTIIGGLNWGLVGLFGFDLVASLFGMMTVLS
                     RIIYVVVGLAALYMVALAPKFASRSSEMG"
     gene            complement(1578..>3064)
                     /locus_tag="PLH96_02630"
     CDS             complement(1578..>3064)
                     /locus_tag="PLH96_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB60682.1"
                     /translation="YDNVESNEGAVFVFSEPPTPSAFSPASGTTINNTTPTITFSLNK
                     NGDCKCSLTDQAYGEMETDCSGDGTKNISCPVPDLGLDGAKTVYISCKDTMNSADNSE
                     TNEHLSYTLDTTPPSVSLYSPANDTTDVSLTTDLELTFDEAVNVDAGNIVIYKSSDDS
                     VIETIDVTSDQVSGSGTAVITINPTTDFSYETKYYIQIDPGALVDAINNSYSGITEAT
                     TWTFTTEDVPICPVVAHALTYDAYPTCNPTSCKPGYVLSAGVCKLSSGGSFSILSLGR
                     DDSDEHISTSSTEHIAQIKTQGTDMQLNYQFSRDLKLSTSNEEVRELQKYLNAHGFTV
                     ATSGPGSPNHETTYFGPATRAALSRFQEANNITPALGYFGPLTRAKMFLASASSTQLA
                     TGTLPTATSTIATSQLFLNSLSFGQTHSDVLLLQQLLNTDLATRLAQTGVGSPNHETS
                     YFGSLTLAAVKRFQIKYDIAQAGDRGFGHVGPKTRAELNKLGVR"
     assembly_gap    3065..3164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3165..4319)
                     /locus_tag="PLH96_02635"
     CDS             complement(<3165..4319)
                     /locus_tag="PLH96_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HPB60683.1"
                     /translation="MTLVVFQPAWAATFSSLPAAPTEEQSAQGTGEINEETTEHLINS
                     LAGREYQVTWQDKDYQGDDFATGKWHFANRANNLRAYFDESGFEMMPRVISPNEPAPW
                     NFKYSPLTLQRENDNQPQALNQVTNLLAEDNKIIATRGEGVEEWYLNTPAGLEHGFNL
                     QNRPAGDGPVILKGNITTDLEATLVDNNKITFQQNGKLILSYEKLKVIDNEGTELPVR
                     LELEKEGDSNTYQLNFFFDDTNAVYPIIIDPLLDSSWQAESNQASAQFGRVVDSAGDV
                     NGDNYDDIIVGAYKYDNGQTDEGAVFVWYGSATGLGDNGTTTNADWQAESNQASAQFG
                     HSVASAGDVNGDNYDDIIVGASIYDKGQTNEGAVFVWYGSETGLGESGTPA"
BASE COUNT         1252 a         1009 c         1049 g         1282 t
ORIGIN      
        1 taccggtttc tctaccaggg tcggctcaaa accatttgat tcgccggcaa tccgcaagac
       61 atctttgtca acttttttca gttctcggta agccgaattg aagtggttaa ccgtcgtgcc
      121 aagcgaaccg ccgagtttgg ctaaataatt ttcataggcg ccaatgtgct tgcccaactc
      181 ttccaccctc tttttgattt ctttggcgga ttcttcaatc tgtaacgctt ttaagccctg
      241 caaaaccgtc tgcaagtaag ccaaaaagga agtcggcgac acgataatca catgatattt
      301 ggcagccgcc cgttgaatca aattttcggt gttttcatct ttcagcgcgc cgattttgtt
      361 gacaatcaaa tcataataaa ttgcctcatg cggaataaac ataaaagcaa actccagcgt
      421 tcccttgtcg ggctgaacat atttagccgt ctcttggatt cgcaacttga ggtcgttgac
      481 aaagactttc tccaaacggt cgcgctcggt ttcgtttttt tcttcggcca aacgattata
      541 attttctaac gaaaattttg agtctatagg aattattttg tctttgacga acaccgccgc
      601 gtcgacaatc gtgccgtccg cgaacgggta ttgcatctgg taactgcccg gaggcatcac
      661 gttcttgagc aaggtttcta aataatattc acccagtatc cccctttgtt tcggattctt
      721 caaaatatct tgcaacgatt gcaactggtc ggcaaaaccg accacctgac gattagtttc
      781 atcaagcttg gttagacgtt cggtcacgtc acgaataatt ttggacgatt cggaaaattg
      841 cgtttggatg gaattctgca tgtggcgcgc cgactcaccc aacttgtcat caaccgtttt
      901 gattaaggtg ttgagctggt tttgcaagag caagagagat tcagctgagg cattgttggc
      961 tccattttgg tcatctttta agcctcgccg accaaacaaa agccaggcca aaaacacgcc
     1021 tccggcgccc aaaattagcc ctagaacgat aaaaaggatt gaatcggtca tcttctctat
     1081 cattctaaca gaaaaaccct ccggttcaat cctcttttat cccctctttt gtttgttagc
     1141 ccatttctga cgaccgactg gcaaacttgg gcgctaacgc gaccatatac aaggccgcca
     1201 agccaaccac gacataaatg atccgcgaca acacggtcat catgccaaag agagaagcga
     1261 ctaggtcaaa tccaaacaga ccgaccaagc cccagttgag gccaccgatg atggtcaaaa
     1321 cgatggcgag ccaatctaac gctttaaatt ttcccataga aaaaattgtt agctgttaat
     1381 ttttaatgat aaaacaatat cttttttaat atgtctttaa tttttataaa ctttgaaaat
     1441 gaattttgag ttaatagtag agctaaagaa acaagcaact tttatttttt aattattccc
     1501 ttttgctcaa aataggcctg acctcttttg ggacaaggct tgttacgtaa catgctacgt
     1561 aacaaaccta taccagacta acgaactccc agtttgttca gttccgctcg ggtcttgggg
     1621 ccaacatgac cgaagccgcg atcgccagct tgggcaatat catatttaat ttggaatcgt
     1681 ttaacggcgg caagggtaag cgagccaaaa tatgaggttt cgtgattggg agagccaacg
     1741 ccggtttggg caagcctggt ggccaggtca gtattgagca gttgttggag aagaaggaca
     1801 tcggagtgcg tttgaccaaa agagagtgag ttaagaaaga gctgagaggt tgcaatggtt
     1861 gaggttgcag ttggcaatgt tccggtcgct agttgggttg aagaggcgct ggcaagaaac
     1921 atttttgctc gggtcagagg gccaaagtac cccagggcag gtgtaatgtt gtttgcttct
     1981 tggaaacgac taagagcggc gcgagtggct ggaccaaagt atgttgtttc gtggttagga
     2041 gagccagggc cgcttgtggc aacggtaaag ccatgggcat taagatattt ttgcaattct
     2101 ctaacttctt cattggacgt actcagcttt aagtctcgag aaaactgata attgagttgc
     2161 atatctgtgc cctgggtttt gatttgggca atatgctctg ttgagctggt tgaaatgtgc
     2221 tcgtctgaat cgtcacggcc aagggagaga atggaaaaag atccgccact ggaaagcttg
     2281 cagacgccgg ccgacaaaac ataaccgggt ttacagctgg tcgggttgca ggttgggtag
     2341 gcgtcataag tcaaagcgtg agcgaccacc ggacaaattg gcacatcttc agtcgtgaaa
     2401 gtccaagtcg tcgcctcggt tattcccgaa taactattat tgatagcgtc aaccaaggcg
     2461 cctgggtcaa tttggatata atattttgtt tcgtaactaa aatcagtggt tggattgatg
     2521 gtgataaccg cggtgcctga accactgacc tgatccgagg tgacatcaat ggtttcaatc
     2581 accgaatcat cgcttgattt ataaataaca atattgccgg catcaacatt aaccgcctcg
     2641 tcaaaagtta gttctaggtc agttgtaagg gagacatctg ttgtatcatt ggctggcgaa
     2701 taaagcgaaa ctgaaggtgg agttgtgtcc aaggtatagc ttaagtgttc gttggtctca
     2761 ctgttatcag cgctgttcat agtgtcttta caggaaatat agactgtttt ggcgccatcc
     2821 aggcccaaat caggcaccgg acaactaata ttttttgtgc cgtcacccga acaatctgtt
     2881 tccatctcac cgtaagcttg gtcagttaaa gaacatttac aatcaccatt tttgtttaaa
     2941 gaaaaagtga tggttggcgt ggtattatta atggttgtcc cagaggctgg tgaaaaagct
     3001 gatggtgtgg gaggctcgga aaagacaaac accgcgcctt cgtttgactc gacattatcg
     3061 taatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagccgg agtgccagac
     3181 tcgcccaaac cagtctccga gccataccag acaaacaccg cgccttcgtt tgtctgacct
     3241 ttgtcgtaaa tcgaagcgcc aacgataata tcatcataat tatcaccatt aacatcacca
     3301 gctgaggcga cggaatggcc aaactgggcc gaggcctgat tggactcagc ttgccaatca
     3361 gcgttagtcg tggtgccatt gtcacccaaa ccagtcgctg aaccatacca gacaaacacc
     3421 gcgccttcgt ctgtctgacc attatcatat ttataagcgc ccacaataat atcatcataa
     3481 ttatcaccat taacatctcc agctgagtcg acaactcggc caaactgggc cgaagcctgg
     3541 tttgactcgg cctgccagct tgaatcaagg agcgggtcaa taattatggg ataaacagcg
     3601 ttggtgtcgt caaagaaaaa attaagttgg taagtattgc tgtcaccttc tttttctaat
     3661 tccagtctaa ccggcaactc cgtcccttcg ttatcaatga cttttaattt ttcataactg
     3721 aggattaatt tgccattttg ttggaaagta attttattgt tatcaaccag ggtggcttct
     3781 aagtcagtgg ttatattgcc cttgagaatc actggcccat cgccggccgg gcggttctgt
     3841 aggttaaaac catgttcaag accggcggga gtattgagat accattcctc cactccttca
     3901 cctctggtgg caatgatttt gttatcttca gccaagaggt tggtgacttg gtttaaagct
     3961 tggggctgat tgtcattttc tcgttgtaag gttaggggtg aatatttaaa attccaaggc
     4021 gctggttcat tcggggaaat aactctgggc atcatttcaa aaccagattc atcaaaatag
     4081 gcccgtaaat tattggcccg attagcaaaa tgccacttgc cagtggcaaa atcatcacct
     4141 tgataatctt tgtcttgcca agtgacttga tattctcggc cagccaaact gtttattaaa
     4201 tgctcggtgg tttcttcatt tatctctcca gtgccttggg ctgattgttc ctctgttggc
     4261 gcggcgggca aagaggagaa ggtggcggcc caggccggct gaaaaacaac cagagtcatt
     4321 gataaaatga gcacggagga gacaagggag tttggttttt tttgagagtg cctcatacta
     4381 tcttgcgcaa aataagccaa ctcttaaccc caggatgagt cagtctaacc atggttaaag
     4441 tataacacaa gccagaggag ataaatagat tctccgcaag taggtgtgga taaagtttct
     4501 ggccagaacg aagaggtggg aataggccta acctcttttt gacagggaac tgttacgtag
     4561 cttgttacac aacaagttga aatatgcttt tcttttttat gtttttattt tataatctta
     4621 gatatggcaa aacgaaaaag tcaggatatt aaaaacaaaa cttcgcgctc cagaaaaacc
     4681 aaaaaacgct tg
//
LOCUS       DAOOLO010000037         4569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_22784_length_4569_cov_6.229951, whole genome shotgun sequence.
ACCESSION   DAOOLO010000037 DAOOLO010000000
VERSION     DAOOLO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22784_length_4569_cov_6.229951"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLH96_02640"
     CDS             <1..365
                     /locus_tag="PLH96_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60684.1"
                     /translation="RTALIVLFGLLIVIFGFARESTSGFSYNQEVAAESTSDWELASE
                     TEMTARPRPMVAEEETVAITSESADESLANEELPEFVTEMNKVKKRQPRRKKSGLAGE
                     GPKIEVTNFDAPEEDEKQ"
     gene            337..1518
                     /locus_tag="PLH96_02645"
     CDS             337..1518
                     /locus_tag="PLH96_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPB60685.1"
                     /translation="MRRKKTKNNKRWWTAAAVGVAAFGLLFFWFWQSSDAPIYDRGTT
                     GLVSSDLLAQTTSNQKEEPKLIVTHKKTPAVVKAVYMSSWVAATKNIRERLINNLAGT
                     EINSIVIDVKDYSGAVSMDINTKTAGQLSGRVKDLAEFVDYLHQKNYYVIGRISVFQD
                     NLLSRQRSDLALKRLDNGALWTDRKGIAWLDVSAPEVWDYTIAVAKEAYRLGFDELNF
                     DYIRFPSDGNTSNVAYPYSRLSVDTRAEALRKFFVYLNAKMKEIDAPISADVFGMVAT
                     NTDDLGIGQVLENAIPYFDYVAPMVYPSHYPPGFLNYKNPASVPYEIVNHSMGVAATR
                     VVAQGYSPLKLRPWLQDFDLGADYTAEMIKAQKRAVYDAGLTSWMMWDPANIYTVGAY
                     R"
     gene            1708..3459
                     /locus_tag="PLH96_02650"
     CDS             1708..3459
                     /locus_tag="PLH96_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPB60686.1"
                     /translation="MNNQTVNYFAQTDFRGKRTTFGIKSDDRARHFYVIGKTGLGKST
                     LLENMAVQDIKNGEGVAFIDPHGKSAETLLDFVPPERIKDVIYFAPFDLDYPVAFNIM
                     EDVGKDQRHLVANGLMSTFKKIWPDVWSARMEYILNNTLLALLEYPNSTLLGVNRMLS
                     DKVFRQTVVNNISDPSVKSFWVDEFAKYNERYMQEAGDAIKNKIGQFTSNPLIRNIIG
                     QPKSTFDLRRAMDEKKILIVNLSKGRLGEQNANLLGGMFITKIYLAAMSRAEISPSAL
                     SRLPQFYLFVDEFQSFVNDSFADILAEARKYKLCLTIAHQYVEQMPETVRNAVFGNVG
                     SIISFRVGPFDAELLEKIFAPTFTKEDLVNLGFAQIYLTLMIDGVGSPPFSAVTIPPI
                     VALDESNRKAIVDYARKTYAKPRDQVQKEITGWYGEEVGNSSNKKQEGPKAGESKKKD
                     DGQQSFKPFVQVKPPNVGKNVIPKINNNNNVPRKFSLDILRHKPQASGHKEDLKSVLK
                     EILADKKNKKEEPPKKSTEVKKEELGKKETVQKLTAPVEKKQEEKSEKTPMTKNEAEK
                     QEIPQNVLEDILRVDLE"
     gene            3479..>4569
                     /locus_tag="PLH96_02655"
     CDS             3479..>4569
                     /locus_tag="PLH96_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB60687.1"
                     /translation="MENKKTILIFSTAYLPLVGGAELAVKEITDRLPEYDFCLVTARL
                     RRDLPKQEKIGQVEVFRLGLGLWLDKIILAFWGGFWASKRFKNRRIDTVWGIMASFGG
                     LAALAYKRHTGTPFLLTLQEGDDLVEVEKKARILGRRFRAMFREADFIQAISTYLKDW
                     ARRMGATCPIVVVPNGSKCDPNIRIQVSDTNKNNHNNLKPILIITTSRLVKKNAVDDL
                     IKALSFLADNFKLKVVGAGPDEQKLRNLIHDLRLEERVEFAGLVTPEKIFAHLAGADI
                     FVRPSLSEGLGNSFLEAMAVGLPTIGTPVGGIPDFLVDNETGWFCEPRNPQSIAEKIK
                     FIVDPANHELVNQVVEKARQLVVEKYNWD"
BASE COUNT         1284 a          888 c         1185 g         1212 t
ORIGIN      
        1 tgcgcacggc tcttattgtt ttgtttggtt tgttgattgt tatttttggt tttgcacggg
       61 aatcaactag tggcttttct tataatcaag aagtagcggc tgaatcaaca tcggactggg
      121 aattggcgtc ggaaacagaa atgacagcaa gaccaaggcc aatggtggca gaagaagaaa
      181 cggtggccat aacctccgaa tcagctgacg agtcgttagc gaatgaagag ctgccagagt
      241 ttgtgactga aatgaacaaa gtcaaaaagc gacagccgcg tcgtaagaag tcggggctag
      301 ccggagaagg accaaaaatc gaggtaacca attttgatgc gccggaagaa gacgaaaaac
      361 aataagcgat ggtggacggc ggcggcggtt ggcgtcgccg cttttgggtt gttgtttttt
      421 tggttttggc aatcttctga cgcgccgatt tacgatcgtg ggacgaccgg cctcgttagc
      481 agcgatttgt tggctcaaac tacttccaac caaaaagagg aaccaaaatt gatcgttacg
      541 cataaaaaga cgccagcggt ggtcaaggcg gtttatatgt ccagttgggt ggcggcgacc
      601 aaaaacattc gcgagcgcct catcaataat ttagcgggca cagaaataaa ttctatagtg
      661 attgatgtca aagattattc gggtgctgtt tctatggata taaacactaa aacggccggt
      721 caactaagtg ggcgagtcaa agatttggcg gagttcgtgg attatctgca ccaaaaaaat
      781 tattacgtga ttggacgcat ttctgttttt caagataatt tgttgtcacg gcaaaggtct
      841 gacttggcgc ttaaacgctt ggataatggc gcgctttgga ctgaccggaa gggaattgcc
      901 tggttggatg tcagcgcgcc ggaagtgtgg gattacacga ttgccgtagc caaagaagca
      961 taccgccttg gttttgatga attgaatttt gattacatcc gtttcccctc tgatggcaat
     1021 acgagcaatg tcgcctatcc ctattcacgc ctaagtgttg ataccagagc cgaagctctg
     1081 cgtaagtttt ttgtttattt gaatgccaaa atgaaagaga ttgatgcgcc aatttcggcc
     1141 gatgtgtttg gcatggtagc aactaacacc gacgacctgg ggattgggca agttttggaa
     1201 aacgcgattc catattttga ttatgtcgcg ccgatggttt atccatcaca ttacccgcca
     1261 ggctttttga attacaaaaa tccggcctcc gtgccctatg aaatagtgaa ccattcaatg
     1321 ggggtggccg ccacgcgggt ggtggcgcag ggttattcgc cgcttaaact taggccttgg
     1381 ttgcaagatt ttgatttggg ggctgactac acggctgaga tgattaaagc gcaaaaacgc
     1441 gccgtttatg atgctggcct aacttcttgg atgatgtggg acccggcaaa catctataca
     1501 gtgggggcgt accggtaatt ggagaagagt cgtcatcaac tcttgactaa agtcgggctg
     1561 ggtcaggtta attttgcaac tggcgatttt ttgttcaact ggaattatta acaattaagt
     1621 tttataatta ggacagcaaa attacattat tttctagttt tctcatttat ataatttaca
     1681 ggcatttgtg tacgggagtc gcattttatg aataatcaaa ccgtcaatta ttttgctcag
     1741 actgatttcc gtggcaaacg gacgactttt ggcatcaagt ctgacgaccg tgcccgccac
     1801 ttttacgtga ttgggaagac tggtctaggc aaatcaactt tgcttgagaa tatggcggtg
     1861 caggatatca aaaacggcga gggggtggcc tttattgacc cgcatggcaa gtcagcggaa
     1921 actctgcttg attttgtgcc gcccgagcga atcaaggatg taatttattt tgcgccgttt
     1981 gacctggatt acccagttgc ttttaatatt atggaggacg tggggaagga tcaacgacat
     2041 ttggtcgcca acggcctaat gtctactttc aagaaaatct ggccggatgt ttggtcggca
     2101 agaatggaat atattttgaa taataccttg ctcgcgcttt tggaatatcc aaattcaact
     2161 ttgcttggtg ttaatcggat gttatcggat aaggtattcc gacaaacggt ggtgaacaat
     2221 atttctgatc cctcggtcaa atcgttttgg gtagacgagt ttgccaaata caacgagcgt
     2281 tacatgcaag aggcgggcga cgccattaag aacaaaattg gtcaatttac ttccaaccca
     2341 ttgattagaa atattatcgg tcagcccaag tccacttttg atctgcggcg ggcaatggat
     2401 gaaaaaaaga ttttgattgt caacttgtcc aaggggcgtt tgggggagca aaacgccaat
     2461 cttttggggg gaatgtttat taccaaaatt tatctggcgg ctatgtcgcg cgccgaaatc
     2521 tctccttccg ctttgtctcg tttgccgcaa ttttatcttt ttgtggatga gtttcaatct
     2581 tttgttaacg attcgtttgc tgatattttg gcggaagcgc gcaaatacaa actgtgtcta
     2641 accatcgcgc accagtatgt tgaacaaatg ccggaaaccg tccgcaacgc cgtctttggc
     2701 aacgttggga gcatcatctc cttccgggtc ggaccgttcg acgccgagtt gttggaaaaa
     2761 atctttgccc cgactttcac taaagaagac ttagttaatc tgggttttgc gcaaatctat
     2821 ctgactttga tgatagacgg tgtcggatca ccgccttttt cggcggttac cattccgccg
     2881 attgtggcct tggacgaatc caaccgcaag gcgattgttg attatgccag aaagacttat
     2941 gccaaaccgc gcgaccaagt ccaaaaggaa attacaggtt ggtatggcga agaggtggga
     3001 aattcaagca acaaaaagca agagggccca aaggcggggg aatctaaaaa gaaggatgat
     3061 ggtcaacaat cgttcaaacc atttgtccaa gtcaagccgc ctaacgttgg gaaaaatgta
     3121 atacccaaaa taaacaataa caacaacgtg cccaggaaat tttcgctgga tattttaagg
     3181 cacaagccac aagctagcgg tcacaaagaa gatttgaaat cagtgctaaa agaaattttg
     3241 gcagacaaga aaaacaaaaa agaagaacca ccgaagaaat cgaccgaagt caaaaaggag
     3301 gagttgggga aaaaagagac agttcaaaaa ttgacggcgc cggttgaaaa aaaacaggaa
     3361 gagaaatcgg agaaaacgcc aatgacaaaa aacgaagcgg aaaaacaaga aataccgcag
     3421 aatgtcttgg aagatattct cagggttgat ctggagtaaa cttgtgtata attggaaaat
     3481 ggagaacaaa aaaacaatcc tcattttttc aacggcttat ctgccgttgg tgggcggggc
     3541 ggagctggca gttaaagaaa ttaccgatcg cttgccggag tatgactttt gcttggttac
     3601 ggcccgtctt cgtcgtgatt taccaaaaca ggaaaaaatc ggccaggtag aggtttttcg
     3661 gctggggctt ggtttgtggc tagacaagat tattctagcg ttttggggtg gtttttgggc
     3721 ttctaaaagg tttaaaaaca gacgaattga cacagtttgg gggattatgg ccagttttgg
     3781 tgggctagcg gccttggctt acaagagaca caccggaacg ccttttttgt tgactttgca
     3841 ggagggtgat gacttggtcg aagtggagaa aaaggcgcga attttgggcc ggcggtttag
     3901 ggcgatgttt agagaggccg actttattca agctatttcc acttatctaa aggattgggc
     3961 gcggcgaatg ggggcgactt gcccaattgt cgtggtgcca aatggaagca aatgcgaccc
     4021 taacataaga attcaggtga gtgacacgaa taaaaataac cataacaacc taaaacctat
     4081 tctaattatt accacttcgc ggttggtaaa aaagaacgca gtggacgatt tgattaaagc
     4141 cctgtccttt ttggcggaca atttcaaatt aaaagttgtc ggcgccggtc ccgacgaaca
     4201 aaaattgaga aacttaatcc acgatttaag attggaggag agagtagaat ttgcgggttt
     4261 ggtgacgcca gaaaaaatct ttgcccattt ggccggggcg gacatttttg ttcggccgtc
     4321 attgtcagaa gggctgggca attctttcct agaagcgatg gcggtcggtt tgccaacgat
     4381 tggcacgccg gtcggaggga ttccggattt tttggttgat aatgaaactg gttggttttg
     4441 cgaaccgcga aatccgcaat caattgctga aaaaattaaa tttattgtcg accctgccaa
     4501 tcatgagctg gtaaaccaag tggttgagaa agctcgccag ttggtggttg aaaaatataa
     4561 ctgggacaa
//
LOCUS       DAOOLO010000038         4527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_23092_length_4527_cov_6.325134, whole genome shotgun sequence.
ACCESSION   DAOOLO010000038 DAOOLO010000000
VERSION     DAOOLO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23092_length_4527_cov_6.325134"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /gene="rplT"
                     /locus_tag="PLH96_02660"
     CDS             <1..223
                     /gene="rplT"
                     /locus_tag="PLH96_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB60688.1"
                     /translation="AGVYSFTHRRDKKNDFRRLWNIKIGAALSEYDLSYSQFIGLLKK
                     KDIALNRKMLADLAENEPTVFAEIVSSVK"
     gene            complement(298..939)
                     /locus_tag="PLH96_02665"
     CDS             complement(298..939)
                     /locus_tag="PLH96_02665"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60689.1"
                     /translation="MGKIDYNDSRNAHRRIKICVSGAADTTHCGDGALDIAKELGREI
                     VRQGAVIVTGATTGWPLWSTIGAKEEGGLSIGVSPASTEKEHVEEWGLPIDYLDVIIY
                     TGQGFSGRDILLTRTTDAVILGCGRIGTIHEFTVAFEDGKPIGILEGCWDTDETLKTI
                     LERGHRPNDKIVFDSDPKRLVAKIIEMVETDKERLNKEGLMKGIQSQDGVKRG"
     gene            complement(978..2351)
                     /locus_tag="PLH96_02670"
     CDS             complement(978..2351)
                     /locus_tag="PLH96_02670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB60690.1"
                     /translation="MDKKKTRIDFFGGAESVTGSNFLVTDNKTDLKILIDCGLFQGPS
                     ALEDLNYESFPFEPAQIDFLLVTHAHLDHIGRIPKLVRDGFSGKIYSTPPTKEISEYS
                     FFDSLNVQNKSGGGDKDKILFNEQDIKKALALWETVEYDQSFNLGEYQIVAKDAGHIL
                     GSTMWGISRNGRSLIFTGDLGNSPSNLLKDTEPLGPVNYVVMESVYGDRNHEGKKARR
                     SLLEDVIEETMKKGGTLMIPAFSIERTQQILYEIEGMMENSRIPLVPVFLDSPFGIEV
                     TRIYKKYAKYLNDDVRDSIESGNEIFRFPQLQMTLSTEQSKAIFNANPRKIIIAGSGM
                     STGGRILHHEKRYLSDPKSTLLLIGYQQVGTLGRLIQDGAKTIKIMGEEIPVNARIAV
                     IHGYSSHKDSDHLLHFVDESADTLEKVYLAMGEPKSALFLAQRIRDYLGIDARVPKAG
                     EKVEIDV"
     gene            complement(2327..2761)
                     /locus_tag="PLH96_02675"
     CDS             complement(2327..2761)
                     /locus_tag="PLH96_02675"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPB60691.1"
                     /translation="MKFKGFFILSLILFATIVLLDFFAFKYFLYWHFRWFDKPMHFLG
                     GFLVALVALQLYFALSTRPLTGWRLVALSVFPALLVGCLWELFEFTAERFYFSAITLK
                     TFGMLYGGWRDALRDLFFDLLGSLSAASLFITNLIWIKRKPV"
     gene            complement(3215..3799)
                     /locus_tag="PLH96_02680"
     CDS             complement(3215..3799)
                     /locus_tag="PLH96_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPB60692.1"
                     /translation="MQNPKEEKTFLMIKPDGVRKGLVGEIIKRLEQRDLKIMALEMFQ
                     PTVEKIDNHYPKDENWRRRLGQKTLATYEKYGYDPIEELGTNDDLVIGNKVRDWLIDY
                     MTSAPLVRMVVQGVHAVSMVRKIVGPTIPSTAEMGTIRGDFSADSPALANREKRAVMN
                     LVHASETAEEAEHEIKHWFGDSPIYDYKRFGVDE"
     gene            complement(3834..>4527)
                     /gene="map"
                     /locus_tag="PLH96_02685"
     CDS             complement(3834..>4527)
                     /gene="map"
                     /locus_tag="PLH96_02685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB60693.1"
                     /translation="LRQVAELVKPETETVILDDLALDLIRQGGDDPAFLNYRPSGSRL
                     AYPASLCVSVNEEIVHGLPGKRVLQAGDIVGLDLGLKHKGLFTDMAVTVPVDKVGRED
                     QKLIRVAAKALEIGIGAVKPGGRVGDISATIQRYVENEGLNVVRELAGHGVGYAAHED
                     PFIPNYGRPGTGEKLKPGMVLAIEPMVTAGSGKIVLEADDFTFSTKGGEKSAHFEKTV
                     VVTEKGALVLTK"
BASE COUNT         1306 a         1155 c          829 g         1237 t
ORIGIN      
        1 ggccggtgtt tattctttca cccatcgtcg cgacaagaaa aacgacttcc gccgtttgtg
       61 gaatattaag attggcgccg ctctcagtga atatgatttg tcttacagcc aatttattgg
      121 tctactcaag aaaaaagata tcgctttaaa tcgcaaaatg ttggccgacc tggccgaaaa
      181 tgagccaacc gtttttgcgg aaatcgtctc tagtgttaaa taaccaaaat ataaccaaaa
      241 taaaaaacgc ccctaggggc gttttttggt tattttaaca aaacttttac agtcaaatca
      301 ccccctcttc actccatcct gactttgtat ccccttcatt agcccctctt tgttcaatct
      361 ttctttgtcc gtctccacca tttctataat cttggccacc agtcttttgg ggtcgctatc
      421 aaaaacaatt ttatcatttg gacgatggcc ccgttccaag attgtcttta gggtttcgtc
      481 ggtgtcccaa catccttcca aaatgccaat cggtttcccg tcctcaaacg cgaccgtaaa
      541 ctcgtggatt gttccaattc ggccgcaacc caaaataaca gcatccgttg tcctggtcaa
      601 caaaatatct cgtcccgaaa aaccttggcc agtataaata atcacgtcca aataatctat
      661 cggcaaaccc cactcctcca cgtgctcctt ttcggtagag gccggcgaaa caccaatgga
      721 caaaccgcct tcttccttgg cgccaattgt ggaccataac ggccaaccag ttgtcgcgcc
      781 agtcacaatt accgctccct gacgaacaat ctctcgcccc aattctttag caatatccaa
      841 agcgccatca ccacagtggg ttgtatcagc cgcccccgag acacaaattt ttatacgacg
      901 gtgagcgttg cgcgaatcat tgtaatctat tttccccatt ttttatacta taattttaac
      961 atctatcttt ataaaaacta aacgtcaatt tccaccttct ccccggcctt tggcacccgc
     1021 gcgtcaattc ccaaataatc acgaattcgc tgagccaaaa aaagcgcgga cttgggttcg
     1081 cccatcgcca aataaacctt ttccaaagta tcagccgatt catccacaaa gtgaagcaag
     1141 tggtcggaat ccttgtgaga ggagtagccg tgaatcaccg caattcgggc attgacgggg
     1201 atttcttcac ccataatctt tattgttttc gcgccatcct ggatcaggcg tcccaaagtg
     1261 ccaacttgct gatatccaat caaaagaagc gtgctcttag ggtctgatag gtagcgtttt
     1321 tcatggtgta aaattcgacc gccagtagac ataccggagc cggcgatgat gattttacgt
     1381 gggttagcgt tgaaaatcgc ttttgattgt tctgtgctaa gagtcatctg taattgcggg
     1441 aaacgaaaaa tttcattgcc gctttctatt gaatcacgca cgtcgtcgtt gagatatttg
     1501 gcgtattttt tataaatcct agttacctca ataccgaaag gagagtctag aaaaactggc
     1561 accaacggaa tccgcgaatt ctccatcatc ccctctatct catacaaaat ctgttgcgtc
     1621 cgttcaatgg aaaaagccgg aatcattaag gtgccgccct ttttcattgt ttcttctatt
     1681 acgtcttcta acaagctcct gcgcgccttc ttgccttcgt ggttgcggtc gccgtaaaca
     1741 ctctccatca ccacataatt gaccgggcca agaggttcgg tatctttgag aagattagat
     1801 ggagaattgc ccaggtcgcc ggtaaaaatc aaagaacgtc cgttgcgtga aatcccccac
     1861 attgttgaac cgaggatatg gccggcatct ttcgccacaa tttgatactc tcccaaattg
     1921 aaagattgat catattcaac cgtttcccac aacgccaaag ctttttttat gtcttgttca
     1981 ttaaacaaga ttttatcttt gtcaccacca ccacttttgt tttggacatt caaactgtca
     2041 aaaaaactat attccgaaat ttccttggtc ggcggcgtgg aataaatttt gccagaaaaa
     2101 ccgtcgcgaa ccaatttggg aattcggcca atgtggtcta ggtgcgcgtg agttactaaa
     2161 agaaagtcaa tttgcgccgg ttcaaatgga aaagattcat aattcaagtc ttccaaagca
     2221 gacgggcctt ggaataaacc acaatcaatt aaaattttta aatcggtttt gttgtcagta
     2281 accaaaaagt ttgagccggt tacactttcg gcgccgccga aaaaatctat acgggttttc
     2341 tttttatcca tattaaatta gtgataaata aactggccgc cgacaacgaa ccaagcaagt
     2401 caaaaaacaa atcgcgcaaa gcgtctcgcc aaccaccata aagcatcccg aacgtcttta
     2461 aggttatagc tgaaaaataa aacctttcgg cggtaaattc aaaaagttcc cataaacaac
     2521 caaccaggag ggccggaaag acgcttaggg ccacaagccg ccaaccagtt agaggcctag
     2581 ttgacagggc aaaataaagt tgcagcgcca ctaaagccac caagaaaccg cctaaaaaat
     2641 gcatcggttt atcaaaccag cgaaaatgcc aatacaaaaa atatttaaaa gcaaaaaaat
     2701 ccaataagac aatggtggca aacaagatta ggctcaaaat aaaaaaaccc ttaaatttca
     2761 ccccctaatt ttaacaaatt catggcccag cgacaaattt taatttaagc tctattttct
     2821 tgatttcaat agtattctgc ctccaatatt atgacaaaat ccgaaaataa acacctttcc
     2881 ctctggttct tcaccaacaa attttaataa aaagtaaaac aaccccgagg ggttgtttta
     2941 cgaggttatt cggaggttat attccctaga aaaaatctag gtgggtgtcc gcaacaactg
     3001 accacggccc gttggaatat gagactccct aaatataagt tttaagctag aaaatattct
     3061 ccaaacaacc tgacctcatt atatgtctat tcaccaaagg cttcaagcgg gaccgaagaa
     3121 ttggaatata agattttaat caaaaattta tccacgattt aacaaagtcg caaaaagaga
     3181 agtcaggagc taaaatagcc ccttatatcc ctgcctattc atcgacccca aaccgtttgt
     3241 aatcatagat cggcgaatcg ccaaaccaat gctttatttc gtgttccgct tcctcggccg
     3301 tttctgaagc gtgaactaaa ttcataaccg ctctcttctc gcggttagct aaagccggtg
     3361 aatcggccga aaaatcgcct ctaatcgtgc ccatttccgc ggtggaggga atagtcggac
     3421 caacaatctt tcgcaccatg ctgaccgcgt ggacgccttg gacaaccatc cgcaccaaag
     3481 gggccgaagt catataatca ataagccagt cgcgcacttt attcccgatg accaggtcat
     3541 cgtttgtgcc cagctcttca atcgggtcat agccatattt ttcataagta gccaaagttt
     3601 tttggcccaa acgccgccgc caattttcat ccttgggata atggttatca attttttcaa
     3661 ctgtcggttg aaacatttca agcgccatga tttttaaatc gcgctgttct aaacgcttga
     3721 taatctcacc aaccaaacct ttccgaactc catccggttt aatcatcaaa aaagtttttt
     3781 cttcttttgg gttttgcata atacaatttc taaaatactt ataatcttta taattatttg
     3841 gttaaaacca aggctccttt ttctgtaaca accacagttt tttcaaaatg ggcgcttttt
     3901 tcgcctccct tggtgctaaa agtaaaatca tcagcttcca aaacaatttt gcccgaaccg
     3961 gccgtaacca tcggctcaat cgccaaaacc atacccggct tcagtttttc gccggtgccc
     4021 ggtcgtccgt agttaggaat aaatggatct tcatgcgccg cataccctac cccatgaccg
     4081 gccagttcgc gcactacgtt taagccttcg ttctctacat atcgctgaat ggttgcgcta
     4141 atatcgccga cccgtccgcc cggtttaacg gccccaatcc caatctctag cgccttagcc
     4201 gcaaccctga ttaatttttg atcttcacgt ccaactttgt ctactggcac cgttactgcc
     4261 atatcggtaa agaggccttt gtgctttagc cccaaatcaa gcccaacaat gtcacctgct
     4321 tgtaagactc tttttcctgg caacccatga actatttctt cgttcaccga cacgcaaaga
     4381 gacgccggat aggccaagcg tgaaccagag ggccgataat taagaaaagc cgggtcgtcg
     4441 ccaccctgac gaatcaaatc aagcgccaag tcatcaagaa taacagtctc tgtctcgggt
     4501 ttaactaatt cggccacttg acgcaaa
//
LOCUS       DAOOLO010000039         4021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_26988_length_4021_cov_7.298790, whole genome shotgun sequence.
ACCESSION   DAOOLO010000039 DAOOLO010000000
VERSION     DAOOLO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26988_length_4021_cov_7.298790"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..446)
                     /locus_tag="PLH96_02690"
     CDS             complement(162..446)
                     /locus_tag="PLH96_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPB60694.1"
                     /translation="MWYVYMLFCDEKIFYVGITNEPSKRIKQHKNKESFFTKKFSQIR
                     PVYCEKYPTKYQAAEREKQLKGWSKTKKQMLIDGKLGTNVCTELSKLFGE"
     gene            1344..1745
                     /gene="mscL"
                     /locus_tag="PLH96_02695"
     CDS             1344..1745
                     /gene="mscL"
                     /locus_tag="PLH96_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPB60695.1"
                     /translation="MLKEFKQFLLRGNVVDLAVGVVVGAAFGSVVTALVSDLITPFIA
                     AIVKVPDFSDLSFTLNSSRFMYGHFLNALISFLLVAASIFFLIIKPMNFLIAKTRKEE
                     PLDPTTKKCKECLSEIPIDAKRCSHCTQIVI"
     gene            1928..3745
                     /locus_tag="PLH96_02700"
     CDS             1928..3745
                     /locus_tag="PLH96_02700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPB60696.1"
                     /translation="MSNKKIVKKSKPKANLKTDWDLSPLFISDKDPRIEEEQLIARQA
                     ADKFITKWHKRKDYLSQPAALREALKDYDQWLADHGPNSRVCYYFHLRTELNQNDPEL
                     RARFNKAKELAVSIQNDIQFFTLRLAKIPPKTQRELLASPTLADFKHFLEKIFAEGKY
                     TLSEAEEKVINLFHETSYSNWVKMIESLISSETFEGHSFAELLATVNSRDQETRAWAF
                     RAINDILAKHADVAEAEMNSLLAFKKTNDELRGAKRPDELRHLSDDISSEVVDVMSQT
                     VAERFDLPRRYYELKARLLGLPNLKYYEKSVPYGELARNFDYDESVNLVDKVLVKLDK
                     ELAEIFRGFVNNGQIDVFPHTSRANGAFCSNMRKIDPTYILLNHTNQLKDVMTLAHET
                     GHGLNNELIKQKQREIYYETPVSTGEVASTFVEDFALEELGRGSNPQDKLRLVMAKLD
                     DDVATIFRQVAAYRFEHELHQTFRAKGYLSKQEIGDLFLKHMKDYTGAAMEFPTGTEN
                     WWVYWSHFRYYFYVYSYASGLLISKALQQIVRDKPDKISLVKEFLATGSSASPDQIFA
                     KLGLDITKPDVWQAGLAEIERLLNEAEELYNETKDHKIK"
     gene            3946..4019
                     /locus_tag="PLH96_02705"
     tRNA            3946..4019
                     /locus_tag="PLH96_02705"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3978..3980,aa:Ala,seq:cgc)
BASE COUNT         1198 a          952 c          788 g         1083 t
ORIGIN      
        1 aacccgaccg taaacggaaa aaagctatat ttttcaatag cttctccgtt caatttagta
       61 ctagaactag ctgatagtcc taactggctc cgcgggcagg actcctctcg gctttcagct
      121 cgccttgaac tcgccgaaat gctcggggta aacttctcct cctactcacc aaacaacttc
      181 gacaattcag tgcaaacatt tgttcctaat ttaccgtcaa ttaacatttg cttttttgtt
      241 ttgctccagc ccttcaattg tttttctctt tctgccgcct gatattttgt tggatatttt
      301 tcacaataaa ctggcctgat ttgagaaaat tttttagtaa agaaagactc tttatttttg
      361 tgttgtttta ttcttttgct gggttcatta gttatcccaa cataaaaaat tttctcatca
      421 caaaaaagca tatagacata ccacatgttt aaattatatc atcttagact tggcttgccc
      481 ctgcttcccc gaagggcttg cactgagctt gccgaagtgc tccgcgggca ggactcgaac
      541 ctgcgaccaa tcggttacac ttaaccctct tgtttccaaa aggagtggac tatatcatca
      601 cccgttctgg gcgtgaggcg cttcccctca ataagttcgg gatgaatcct taacttattt
      661 aagggtactc tctcccctca ttcgtctaaa acgatggagg gtgcaatcga gatagtctct
      721 gaacctgcct tcaacctgct tcgcaatccg aagctttagc gaaggattgg ctgctgatta
      781 ccccttaggg cttccagcaa ttcacctcat ttttcatccc taaattcctt tagggagctg
      841 cgtttgtgtt ggacactaaa tccaaagtga cacagccgac cgctctacca actgagctac
      901 cgcggatcat ccgtggctag gccacggaat atccaagacc attttcggcc aaggattatt
      961 actctaatag tttcgtttaa tagaaatttt ttagagagat tgagaaagaa catatcttga
     1021 accttcaaga caaacataat aataccaaaa atcccaaaaa aggcaaacac acattgtaaa
     1081 ctcccaggca aggtagggag aggccccaaa ttggctccag ataacgttaa tgaagacgcc
     1141 taaataaaac aagccaagac aaaaaacaac aaatttggca taaaaatgac cgcggattaa
     1201 ggccgtcaac cgaagggcgg tcaggttggc ccccaaaaaa attctcgcct ataccccctc
     1261 tatccaagac accaagacaa tcccaaaaat ttctggtata atgtttaaca ttatttatca
     1321 attaataatt tcttttattt cttatgttaa aagaattcaa acaattccta cttagaggca
     1381 acgtagttga cctagccgta ggtgtcgtgg tcggcgccgc ctttggttct gtcgtaaccg
     1441 ctctagtgtc ggatttgatt actcccttta tcgcggcaat cgtcaaagtg ccggatttct
     1501 cagatttgtc tttcactcta aacagtagcc gttttatgta tgggcacttc ctaaatgccc
     1561 taatctcttt tctgttagtt gccgcgtcaa tctttttctt gataattaaa cccatgaact
     1621 ttcttattgc caaaaccaga aaagaagagc ccctcgaccc cacgaccaaa aaatgcaaag
     1681 agtgtttgag cgaaattcca attgacgcca aacgatgttc tcattgtact caaattgtca
     1741 tctaaataac ctgatatccg attttggcaa aaaataaaac agaaataaca agttaaaaca
     1801 aaaacgcccc aattagggcg tttttgtttc tgacgatgat taggaccgtc tccactagaa
     1861 atttttttat attttcataa tttgaggtaa gatggtaaga cacgtaaaac aaatttaaat
     1921 tatatttatg tccaacaaga aaatcgttaa aaaatccaag ccgaaagcaa acctcaaaac
     1981 cgattgggat ttatctcccc tttttataag cgacaaagac ccgcgaattg aagaggaaca
     2041 gcttatcgcc agacaagccg ccgataaatt tatcaccaag tggcacaagc gaaaagacta
     2101 cctatctcaa ccggccgcgc tgagagaagc gctgaaagat tatgaccagt ggctcgccga
     2161 ccatggccca aacagccgtg tttgttacta ttttcacctc cgcacggaac taaaccaaaa
     2221 tgaccctgag ttgcgcgcgc gcttcaacaa agccaaggag ctggccgtca gcatccaaaa
     2281 tgacatccaa tttttcacgc ttcggctagc caaaattcca cccaagactc aacgtgaact
     2341 tttggccagt ccgacattgg ccgacttcaa acattttttg gaaaaaattt ttgctgaagg
     2401 aaaatatact ttgagcgaag ctgaagaaaa agttattaac cttttccatg aaacttccta
     2461 tagcaattgg gtcaaaatga tagaatcctt aatcagcagc gaaacttttg agggccatag
     2521 ctttgccgag ttgctcgcca ccgtaaacag ccgcgaccaa gaaacacgcg cttgggcttt
     2581 ccgcgccatc aatgacatct tggccaaaca cgccgacgtc gccgaagctg aaatgaattc
     2641 tttgctcgct ttcaaaaaaa ccaatgatga attgcgcgga gccaaacggc cggacgaact
     2701 cagacacctc tccgacgaca tctcaagcga agtggtagac gtcatgagcc aaaccgtcgc
     2761 cgagcgcttt gatttgcctc gccgctatta cgaacttaag gctcgtctcc ttggtctgcc
     2821 caatcttaag tattacgaaa aaagtgtccc ttatggagag ctggcgagaa actttgacta
     2881 tgatgaatca gtcaacttag tggacaaagt tctcgttaag ctagataagg aactggcaga
     2941 gatttttcgc ggttttgtca acaacggaca aattgacgtc ttccctcaca ccagccgcgc
     3001 caacggcgct ttttgttcca atatgcgcaa gattgacccg acttatattt tgctcaacca
     3061 taccaaccaa ctcaaagatg tgatgacttt ggcccacgaa accggtcacg gtttaaataa
     3121 cgagttaatc aaacagaaac agcgcgaaat ttattacgag acgccggttt ccaccggcga
     3181 agtggcaagc acttttgtgg aagattttgc tttggaagaa ctggggcgag gaagcaaccc
     3241 tcaagataag ttaagattgg taatggcaaa attggacgac gatgtcgcca ctatttttcg
     3301 tcaagtcgcc gcttatcggt ttgagcacga gttgcaccaa actttccgcg ccaaaggata
     3361 tctatccaaa caagaaatcg gcgacctttt cctgaaacac atgaaggact acaccggggc
     3421 ggcgatggaa tttccgactg gcaccgaaaa ttggtgggtt tactggagtc attttcgtta
     3481 ctacttttat gtttattctt atgccagcgg cttactaatt tctaaagcct tacaacaaat
     3541 cgtgcgtgac aagcccgaca aaataagtct ggtcaaagag tttttggcaa ccggttcttc
     3601 ggcctcaccg gaccaaattt ttgccaaact aggtttggat atcaccaagc cagatgtttg
     3661 gcaagccggt cttgctgaaa ttgaaagact tttgaatgaa gccgaggaat tgtataacga
     3721 aacgaaagac cacaaaataa aataaaaaat aggaaaaagg ctaagaacca aatccattta
     3781 taccgctact tttctcgcgc ttggcccgtg tcagaaagtt gagaaattac ttattttatg
     3841 gttaaataac tttgcccttc agttaaaatt ccgttgtcaa aagtctaaat cgtttgtcat
     3901 aatagcgcca aacaaccagg cggtaatttt ccgagaggga catttgggcc tatagtctag
     3961 tggtacgacg cggcattcgc attgccgagg cgcgagttcg attctcgcta ggtccaccaa
     4021 a
//
LOCUS       DAOOLO010000040         3839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_28724_length_3839_cov_6.547040, whole genome shotgun sequence.
ACCESSION   DAOOLO010000040 DAOOLO010000000
VERSION     DAOOLO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28724_length_3839_cov_6.547040"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1279)
                     /locus_tag="PLH96_02710"
     CDS             complement(233..1279)
                     /locus_tag="PLH96_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPB60697.1"
                     /translation="MATKTTKGFSIKPKEITKKLLNVLPERNRDILTKRYGLDKNSKR
                     LTLEAIGSEYGITRERVRQIENFGLGMIKKSKTYESATEAFGQIKDLMDEYGSVVHER
                     EFLGSLAKDELTQNHIHFLLVVSDNFTKIKEDEEFHHRWTVNEEKAAKVHQSLRRLCA
                     GFSEDDLIPEPELITKFIKELKDFSNEPNLEGLAPKWLKICKLLARNPLGEWGLNTSP
                     NIRMRGIRDYAYLVLRQNKAPLHFTEVAREIDKTFNKKAHPATCHNELIKDGRFVLVG
                     RGLYALTEWGYSQGTVVDVVMRILKEAGALSKDEIINHVLKERFVKENTILVNLQNAK
                     YFKRDENGRYFLVS"
     gene            complement(1386..>3839)
                     /locus_tag="PLH96_02715"
     CDS             complement(1386..>3839)
                     /locus_tag="PLH96_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPB60698.1"
                     /translation="NPFLPDVVNQTEFVLENVSFDENTVLVGHSLGTVVGLRVLERLK
                     KPIKKFVAVSGLVEPKFKDKDRPAAAFLINWDFDFAKIKKNAGDVVILEDQTDYLINQ
                     NQGENLEAKLNGRLVRFEAKRTHTCGTVEPVVLENVLDTVKVFTTRADTLFGATYVVL
                     APEHNFVQKNLSKFDNKSEVQKYQKQATKQSEIARIAETKEKTGVQLKGAVAVNPANG
                     REVPVFIADYVLPDYGTGAVMAVPAHDERDFAFAKKYRLPIIQSIAKKNAPIKSYLMG
                     ANQISDGELEEVGAKIYEKKPDGDRLLVLSAKKLPAYEKLISEKMTAGYWNEYVGKEI
                     VFLFKDKKDRVTKIVLTPETAEEVDKLANEFSADKKRKFVSPWTWLAENDWYKDLIIH
                     TEAGVLVNAGKKFTGLSSEKAKLAVSRYVRGKQVVKYKLRDWVFSRQRYWGEPIPVVH
                     CDKCGVVPVPEDKLPVKLPKVKSYAPTDTGESPLADIEKWVKTKCPACSGPAKRETNT
                     MPQWAGSSWYYLRYLDPKNETALVSQDKEKYWQEVDVYVGGTEHATRHLIYARFWHKF
                     LYDLGVVSRPEPFAKLKNQGLISGSDGRKMSKRWGNVVNPDDVVKSYGADSLRVYEMF
                     MGPFEAGASWQTENMIGARRFLEKVWQLSKKVESQKGDSLLVVQVHETIKKVGKDITD
                     FAFNTAISALMILVNELEKQPTVSRTDFENLLKLLAPFAPHLSEELWSQLGHKTSIHL
                     APWPTYDAQKTVKQELKIMIQVNSKIRDKIVVPASASEEEILKQARASAGAKRWLEGQ
                     TIKKAFYVKGRLVNFVTNK"
BASE COUNT         1020 a          937 c          758 g         1124 t
ORIGIN      
        1 actcccaaag agcataggcc aaaacgaacg gcaaaaaata gaccgtaaaa ggcaaggcta
       61 aagaaatcca ataccaagcc tggctaagaa agttccctat caggtttaaa ataaaatcca
      121 tcctctaaat aataaaacat tactgagaaa aaaaccacta aacaaagacc ctttcatcca
      181 caatcataaa aacgcggcca aaggccgcgt ttttatgatt ggagacaaga ttttaggaaa
      241 ccaagaagta acgtccgttt tcgtcacgct tgaaatactt ggcattttgc aggttgacca
      301 aaatcgtgtt ttctttgaca aaacgctctt tcaaaacatg gttgatgatt tcgtcttttg
      361 ataaagcgcc ggcctctttc aggatcctca tcacgacgtc aacaaccgtg ccctgagaat
      421 aaccccattc agtcaaagca taaaggccgc gaccaaccaa aacaaagcga ccatctttta
      481 tcaactcgtt gtggcaagta gctgggtgcg cttttttgtt gaaggtttta tcaatttctc
      541 gagccacctc ggtaaaatgg agaggggcct tgttttgccg caacaccaaa taagcatagt
      601 cgcgaatacc gcgcatacgg atgtttggcg aggtgttgag gccccattca ccaagcgggt
      661 tacgcgccaa aagtttgcaa attttgagcc acttcggagc caagccttcc aaatttggtt
      721 cgttggaaaa atcttttaat tctttaataa atttagtgat aagctccggt tcgggaatca
      781 aatcatcttc ggaaaatccg gcgcaaagtc tgcgcaatga ctgatgaacc ttagcggctt
      841 tttcttcgtt gaccgtccag cgatgatgga attcttcgtc ttccttgatt ttagtgaaat
      901 tatcagaaac aaccagcaaa aagtggatgt gattctgggt taattcgtct ttggccaaag
      961 accccaaaaa ttctcgctcg tgaaccacac ttccgtattc atccattaaa tctttgattt
     1021 gcccaaaagc ctccgtcgcg ctctcatagg tcttggattt tttgatcatg cccaggccaa
     1081 aattttcaat ctgacgaaca cgctcgcgcg taattccata ttcgctgcca attgcctcaa
     1141 gggttaggcg tttgctgttt ttgtccaaac catagcgctt ggttaaaata tcgcgattgc
     1201 gttccggcaa aacgttgagt agctttttgg taatctcttt tggttttatg gaaaaacctt
     1261 ttgttgtttt agtggccata agtttttact gataattttt tattttttag cgaccgagtg
     1321 atccttcttt tgaccggtta gttgctgtct ttagttgtat catttatttt agtataagtc
     1381 aaacgctact tattggttac aaaattaact agacggcctt tcacataaaa agctttttta
     1441 attgtctgcc cttcaagcca ccgcttcgcg ccggcgcttg cccgagcctg cttgagaatt
     1501 tcttcttcac tagcgctcgc tggcacaaca attttgtctc ttattttgct atttacttgt
     1561 atcataattt tgagctcttg cttgacggtc ttttgagcgt cataggtcgg ccacggcgcc
     1621 aaatgaatag aggttttgtg ccctaactgt gaccataatt cttcagacaa gtgcggggca
     1681 aacggcgcca aaagcttaag taaattctca aaatcagttc gactgactgt cggctgtttt
     1741 tctaactcgt tgaccaaaat catcaaggca gaaatagcgg tattgaaggc aaagtccgtg
     1801 atatccttgc caactttttt gattgtctcg tggacttgaa caaccagcag gctgtcgcct
     1861 ttctgagatt ccactttttt cgacaactgc caaacttttt ccagaaaacg acgcgcgcca
     1921 atcatatttt cagtttgcca agaagcgccg gcttcaaatg ggcccataaa catttcatag
     1981 acacgcaaac tatcggcgcc gtaagatttt acgacatcgt cagggttaac cacgtttccc
     2041 caacgcttag acattttgcg gccgtcactc ccggaaatca aaccttggtt cttgagtttg
     2101 gcaaaaggtt ccggccggct caccacaccg aggtcataca aaaacttgtg ccaaaaacgc
     2161 gcatagatca aatgtctagt ggcatgctcg gttccaccga catagacatc aacttcttgc
     2221 caatattttt ctttgtcttg gctaaccaaa gcagtctcat tcttcgggtc cagataacgc
     2281 aaataatacc aagaagaacc cgcccattgc ggcattgtgt tggtttcgcg tttagccggt
     2341 ccgctgcaag ccggacattt ggttttgacc catttttcaa tatcggccaa aggtgattcg
     2401 cctgtgtcgg ttggggcata acttttgacc ttcggcaact tgaccggcag cttgtcttcc
     2461 ggcaccggca cgactccgca tttgtcgcaa tgaacaaccg ggattggctc gccccaataa
     2521 cgttgacgcg aaaagaccca gtcacgaagt ttgtatttaa caacttgttt gcctcgcaca
     2581 tagcgcgaaa ccgccaactt ggctttctcg cttgaaagac cggtgaattt tttaccggca
     2641 ttaaccaaaa cacccgcttc agtatggata attaaatctt tataccaatc attttccgcc
     2701 agccaggtcc aaggcgaaac aaattttctt tttttgtctg cagaaaattc atttgccaac
     2761 ttatcaacct cctccgcagt ttccggtgtc aaaacaatct tggtcacccg atctttttta
     2821 tccttaaaaa gaaaaacaat ttccttgccc acatattcat tccaatagcc agcggtcatt
     2881 ttttcgctga tcaatttttc gtaggctggc aacttttttg ctgataaaac caagagacgg
     2941 tcgccgtccg gcttcttttc gtaaatcttg gcgccaactt cttccaattc accatcagaa
     3001 atctggtttg cgcccatgag gtaacttttg attggcgcgt tcttcttggc aatactctgg
     3061 ataattggca atctatattt cttggcaaag gcgaaatcac gctcatcatg cgccggtacc
     3121 gccatcaccg cgccggtgcc gtaatccggc aaaacatagt cagcgataaa aacagggact
     3181 tcacggccat tggccgggtt aaccgcgacc gcgcctttca attgaacacc ggttttttct
     3241 ttggtttccg cgatgcgggc tatctcgctt tgtttagtgg cttgtttttg atacttttgg
     3301 acttctgatt tgttgtcaaa ctttgacaaa tttttctgaa caaaattatg ctccggcgcc
     3361 aagaccacgt aagtggcgcc aaaaagcgtg tcggcgcgcg tcgtaaaaac tttcaccgta
     3421 tccaaaacat tttctagcac cactggctct accgtaccgc aagtatgagt tcttttcgct
     3481 tcaaagcgaa ctagtcggcc attcagcttg gcctccagat tttctccttg attttgattt
     3541 atcaaatagt cagtctggtc ttccaaaata acaacgtcgc cggcattttt tttaattttg
     3601 gcaaaatcaa agtcccaatt tatcaaaaaa gcggcagccg ggcggtcttt gtctttgaac
     3661 tttggttcca ccaaaccaga aactgccaca aattttttaa ttggtttctt gagacgttcc
     3721 aaaacacgca agccgacaac tgtccctaaa ctatgcccaa ccaaaaccgt gttttcgtca
     3781 aaagaaacat tttccagcac aaattccgtc tggttgacga catccggcaa aaaaggatt
//
LOCUS       DAOOLO010000041         3635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_30929_length_3635_cov_4.979330, whole genome shotgun sequence.
ACCESSION   DAOOLO010000041 DAOOLO010000000
VERSION     DAOOLO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30929_length_3635_cov_4.979330"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1202
                     /locus_tag="PLH96_02720"
     CDS             138..1202
                     /locus_tag="PLH96_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPB60699.1"
                     /translation="MKDYSKEIEELVVAVTKEGASDLHLTVGRHPSIRVSGALIPIIK
                     KPILTPEDTLGIAMEILNDENKQILLEQREVDFSYSPAANVRFRGNAFFQRGMVGVAL
                     RLIPMKVPTIKELNLPEQLADFVHKKQGFFLVVGPVGQGKSTTLASLVEMVNHERAEH
                     IITIEDPVEYLFTSDRSIIDQREVRFDTKNFYTALRSMFRQDVNVAMVGEMRTPETIS
                     MAVTAAETGHLILSTLHTNSAAQTIDRIIDSFPGNQQHQIRMQLAGSLLGVFSQRLIP
                     RISGGLIPAFELLINNDAVANLIREGRTAEIDVVIETGSSEGMLDLNRSLAELVRNGE
                     ISADNAFRHSLNPEALNRLI"
     gene            1210..2418
                     /locus_tag="PLH96_02725"
     CDS             1210..2418
                     /locus_tag="PLH96_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB60700.1"
                     /translation="MLFIYKTVDKDGQEQNGSIDAPNIDLAISSLQRRGLIIVSVKPE
                     VTEKKRFHINLPFTRKVSYKEVVMLSRQVATLFEAKVSVLATFRLLAQESLNPVLQKA
                     LLAIVDDIKGGIPISAALSRHPQIFTDFYVSMVRAGEESGKMSEAFVYLADYMDRSYA
                     LSSRAKNALIYPAFVVVSFIVVMILMVIFVVPKLSQVLLETGQELPIYTKLLIWTSDF
                     FVMFSVPIALFLAFIGVYLFRYISTPQGQYAFDRFKINIPYVGRLYRKLFLARISDNM
                     NTMLSSGISMVRVLEISGDVVDNEVYKKILLEAAEAIKAGSAVSSIFARYPEIPGVMV
                     QMIKVGEESGKIGFVLEKLSRFYEREVGNEVDTLVGLIEPTLIVILGVGVGIMLTAIL
                     VPIYNLAQGI"
     gene            2654..3088
                     /locus_tag="PLH96_02730"
     CDS             2654..3088
                     /locus_tag="PLH96_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB60701.1"
                     /translation="MKKTLNKGFTLIELLVVIAIIGILAGVVLTSLGSARNKAKVASA
                     EASMASMRAEAELGVGSTGTYATGICTLETSGGLKRLIDAVNGQGIGDATCHADETYH
                     TAWAVELDYTDSVKEGYFCVDSTGFSGTRATSKGQGTVCAES"
     gene            3164..3343
                     /locus_tag="PLH96_02735"
     CDS             3164..3343
                     /locus_tag="PLH96_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60702.1"
                     /translation="MAAKRNFEFAIDEFYHVFNHGNGDRLKQKGAERGGPYLPFIAFC
                     GIIFKWRLKEILNLQ"
     gene            3310..>3635
                     /locus_tag="PLH96_02740"
     CDS             3310..>3635
                     /locus_tag="PLH96_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60703.1"
                     /translation="MAAKRNFEFAIDEFYHVFNHGNGDRLIFLDDKDKRHFTHLLFLT
                     NGTNTFEFSELNPGSIYQFNRGETLVDVGAWCLMDNHYHFLLKEKVEKGVVKFIQRLF
                     SAYTMY"
BASE COUNT          911 a          713 c          900 g         1111 t
ORIGIN      
        1 aggtttgggt cttggcgcgg atggttatat cgtcaaagcg tcagctacgc cgacagaggt
       61 tgtcaacaaa gtcaaagaaa ttttggccaa tcgcaaatac gcttaatttt tagtttttta
      121 aaattaaaca attttttatg aaggattaca gcaaagaaat agaagagctg gtggtagcgg
      181 taaccaagga aggcgcttcc gacttgcact tgacagttgg tcgccatccg tccatccgcg
      241 tctctggcgc gctcatcccc attatcaaaa agccgatttt gacgccggaa gacactttgg
      301 gcatcgcgat ggaaattttg aatgacgaaa acaagcaaat tttgctggaa cagcgcgagg
      361 ttgatttttc ttattcgcca gccgccaatg tccgtttccg cggcaatgct tttttccagc
      421 ggggaatggt tggggttgct ttgcgtttga ttccaatgaa ggtgccgacc atcaaagagc
      481 tcaatttgcc ggagcagttg gctgattttg tccacaagaa acagggtttc ttcttggttg
      541 ttggtccagt tggccaaggc aagtctacca ctctggcctc gctcgtggag atggtcaatc
      601 atgaacgcgc cgaacacatt attacgattg aagacccggt agaatacttg tttaccagcg
      661 accgttctat cattgaccag cgcgaagttc ggtttgatac caaaaacttt tacacagctc
      721 ttcgttctat gttccgtcag gatgtcaacg tggcgatggt cggcgaaatg cgcacaccgg
      781 aaacgatttc tatggcggta accgcggccg aaactgggca cctgattttg tccactctgc
      841 acaccaacag cgcggcgcag acgattgacc gtatcattga ctcgtttccg ggcaaccagc
      901 agcaccagat tagaatgcag ttggccggct cgcttttggg ggtcttctcc cagcgtttga
      961 ttccgcgtat ctccggcggt ttgattccgg cttttgaact tttgattaac aatgacgcgg
     1021 tggccaactt gattcgtgaa ggtcgcacgg ctgagataga cgtggtgatt gagaccggtt
     1081 cgtctgaggg aatgcttgac ctgaaccgct ctttggcgga attggtccga aacggcgaga
     1141 tttccgcgga taacgctttt cgtcactccc ttaatcccga ggcgcttaat cgtttaattt
     1201 aattttgtta tgctttttat ttacaaaacc gtagataaag atggccagga acaaaacggc
     1261 agcatcgacg cgcccaacat agacttggct atcagttcgc ttcaacggcg aggtttgatt
     1321 atcgtttcgg tcaaaccgga agtgacagaa aaaaaacgtt ttcatattaa cttgcctttt
     1381 acgcgcaagg tctcctataa agaagtcgtg atgctctcgc ggcaggtggc gactttgttt
     1441 gaggccaagg tttccgtttt ggcgaccttt cggttgttgg cgcaagaaag tttaaatcca
     1501 gttttacaaa aagccttgct ggcgattgtg gatgatatca agggggggat accgatttcg
     1561 gccgccctta gtcgtcatcc gcaaattttt accgacttct atgtcagtat ggtgcgggcc
     1621 ggcgaagagt ccggcaagat gtccgaggcg tttgtttatc tggccgacta tatggatcgt
     1681 tcctacgctt tgtcctcgcg cgccaaaaac gccttgattt acccggcttt tgtggtggta
     1741 tcatttattg tggtgatgat tctgatggtt atttttgtgg tgccgaagtt gtcgcaagtt
     1801 ttgctggaaa ccggccagga gctgccgatt tacactaaac tcttgatttg gaccagtgat
     1861 ttctttgtga tgtttagcgt gccgattgct ttgttcttgg cctttattgg tgtttatctt
     1921 tttcgctata tcagcacccc ccaaggtcag tatgcgtttg accgttttaa gataaacatt
     1981 ccctatgttg gccgccttta tcgcaaactt tttttggcgc gcatttctga caacatgaac
     2041 acaatgctct catccggtat ttccatggtg cgcgtgctgg agatttccgg cgatgtggtg
     2101 gacaatgaag tttacaaaaa aattctcttg gaagcggccg aggcgattaa ggcgggaagc
     2161 gcggtttcca gtatttttgc tcgttatccg gaaatccccg gcgtgatggt gcagatgatt
     2221 aaggttggtg aggaatccgg taaaatcggt tttgtcttgg aaaaattatc gcgtttttat
     2281 gaacgagaag ttggcaacga ggtggacacc ttggttggct tgattgagcc gacgctgatt
     2341 gtcattttgg gtgttggtgt cggtataatg ctcacggcta ttctcgtgcc gatttataac
     2401 ttggcgcagg gcatctagtt aaattaaagt gtaaaagtta aaatcaaggg tgaaaactag
     2461 taaaaccagc taatttctct tggttttttg gattttacat tttaatcttt aaattctaaa
     2521 tttcccttta gttatccaca gttttccgtt tcccattatt aacttttcgt gaattgttgt
     2581 aaaatataaa tatataaata aagtcgattt taaagtcgtt taaaagttat taattattaa
     2641 gtaagttttt attatgaaaa aaacgttaaa taagggtttt accttgattg aacttttggt
     2701 cgttattgcc attattggta ttttggcggg agtggtattg accagcttgg gtagcgcaag
     2761 gaacaaggcc aaggttgctt cagctgaagc cagtatggcc tccatgaggg ctgaagccga
     2821 gttaggagtt ggctcaaccg gaacttatgc taccggtatt tgcacccttg aaacatcggg
     2881 tggcttgaag aggcttattg acgcggttaa tggacaagga attggagacg ccacatgcca
     2941 tgcagacgaa acttaccata ccgcttgggc tgttgagttg gattacaccg attccgtaaa
     3001 agaaggttac ttctgtgttg acagcacagg attttctggc acaagagcaa ctagcaaggg
     3061 gcaaggaact gtgtgtgcag agtcatagtt ttagaaggga aaagaaaggg aaaaggggag
     3121 ctacccagct cccctttgat gcgttttgtg gtataatgtt taaatggcgg ctaaaagaaa
     3181 ttttgaattt gcaatagatg agttttatca cgtttttaat catggcaatg gtgatcgttt
     3241 gaagcagaaa ggggcagaaa ggggaggtcc ctacctcccc tttattgcgt tttgtggtat
     3301 aatatttaaa tggcggctaa aagaaatttt gaatttgcaa tagatgagtt ttatcacgtt
     3361 tttaatcatg gcaatggtga tcgtttaatt tttcttgatg acaaagacaa aagacatttt
     3421 actcatttgc tttttctaac aaacggcact aatacctttg aattttctga attaaatcct
     3481 ggctcgatct atcaatttaa cagaggtgag acactggttg atgtgggtgc ttggtgtctg
     3541 atggataatc attatcactt tttactcaag gaaaaagtgg agaaaggggt tgttaaattt
     3601 attcaaaggc ttttttcagc ttatactatg tattt
//
LOCUS       DAOOLO010000042         2647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_47575_length_2647_cov_5.845293, whole genome shotgun sequence.
ACCESSION   DAOOLO010000042 DAOOLO010000000
VERSION     DAOOLO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47575_length_2647_cov_5.845293"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..88)
                     /locus_tag="PLH96_02745"
     tRNA            complement(2..88)
                     /locus_tag="PLH96_02745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ser,seq:gct)
     gene            195..>2647
                     /locus_tag="PLH96_02750"
     CDS             195..>2647
                     /locus_tag="PLH96_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPB60704.1"
                     /translation="MTSDTPLKDISRLNPIQQKALERLGLKTARDLLYHFPSRYEVPG
                     QRKQISELAAGDDAVIFGKILSAKTSKAWRKKIPLGEAVIEDETGKIKAIWFHQPYLA
                     KKVAVGQFAEFRGKVAARKKTGTGAVATTAPELYLTNPEVRDGLAWQAGKESLFYNND
                     NNQNLEEFLVPVYPETRGLSSGWFYYQQKKLLAGGALNDLVDSLPTELLKKFNLPSLK
                     TALVWIHHPKKPTDAEAARKRFAFEEVFFIQLARLRDKKIYQSNPSFSLRTSKTEISK
                     FLKNFPFKPTTAQTKAIEQILADFAADKPMVRLLEGDVGSGKTLVAAAATFAIVNAGY
                     EVSYMAPTEILARQHFESFCQYFAHLNVQVGLLTGKECRKFPSKINPTESTHISRTQL
                     LKWVANGEIPILVGTHALIQKNVKFKNLAFAIIDEQHRFGVMQRAKLVRRSNADKTQI
                     NADNSFLYQDLTYRVRSAIFNVANNLGLGHKELIYQNALTEEFKKQNIPFIREKQVPI
                     FYLGKKIGVYIPDFVIDNKIIVELKALPFLGNSEKKQTWNYLKGSEFKLALLVNFGPQ
                     KLEIERIIYDKARQPSAFYPRESASNQHQVVPHLLSMTATPIPRTLALTIYGDLDLTL
                     LDELPAGRKPIVTKIIPPNKREAAYEHVREELAAGRQAYVICPRIDEPDPEKELAIQA
                     KSARAEAKRLQEKNFPEFEVGLMHGKLKPKDKEETMQEFANGEIDILVSTSVVEVGVN
                     VPNATIIMIEGAERFGLAQLHQLRGRVLRSSHQAYCYIFSDTTNAKSMERLKALVETK
                     SGFDLAEKDLALRGAGTMSG"
BASE COUNT          787 a          656 c          562 g          642 t
ORIGIN      
        1 gcggagaggg agggattcga accctcgagg ggcgttaacc cctacctcgt tagcagtgag
       61 gcgctttcgg ccactcagcc acctctccgt attcttgttg tgtcttattt aattgtcgct
      121 ggcagccaca ttaaagcaca atttgaagct ttgttcaaac aacttttgcc ctcgcttttt
      181 gctataatta tctcatgacc agcgacacgc ccctcaagga tatctctcgt ctcaacccaa
      241 ttcaacaaaa agctctggag cggttggggc taaaaacagc gcgcgatctt ctctaccact
      301 ttcccagtcg ttatgaagtg ccaggacagc gcaaacaaat ttcagaacta gcggccggcg
      361 atgacgcggt aatttttggc aaaatcctct ccgccaaaac cagcaaagcg tggcgtaaaa
      421 aaattccgct gggcgaagcg gtaattgaag atgaaaccgg caaaatcaaa gccatctggt
      481 tccatcaacc ctacctcgcc aaaaaagtgg ccgtaggaca atttgctgaa tttcgcggca
      541 aggtagcagc gcgaaaaaag acgggaaccg gggcggtcgc cacgaccgcc ccggagctct
      601 atctcaccaa cccggaagtc cgtgatggcc tcgcttggca agccggcaaa gaatcgcttt
      661 tttataacaa cgacaacaac caaaacctgg aagaattttt ggttcctgtc tatccggaaa
      721 cccgcggtct atcttccggc tggttttatt accaacaaaa aaaattactt gccggtggag
      781 ccctcaacga tttggttgac tccctgccaa ctgaattatt aaaaaaattt aacctgccct
      841 cgctcaaaac cgcgctggtg tggattcacc accccaaaaa accaaccgac gccgaagcgg
      901 cgcgcaaacg gttcgctttt gaagaagtct tttttattca actggcacgc ttgcgtgaca
      961 aaaaaatcta ccagagcaat ccttctttta gtctaaggac cagcaaaacg gaaatcagca
     1021 aatttttgaa aaactttcct ttcaaaccaa cgacggccca aaccaaagcg attgaacaaa
     1081 tcttggccga ctttgctgct gacaagccaa tggtccgtct tttggaagga gatgtcggtt
     1141 caggcaaaac cttggttgcc gccgccgcta cttttgcgat tgttaatgcc ggctatgaag
     1201 tctcttacat ggcaccaacc gaaattctag cgcgccaaca ttttgaatca ttctgccaat
     1261 actttgccca cttgaatgtc caagtgggcc ttttgaccgg caaagaatgt cgcaagtttc
     1321 cctccaaaat caacccaacc gaaagcaccc acatttcccg aacccaactg ctcaaatggg
     1381 ttgccaatgg tgaaattccc attctggttg gcacccatgc cttgatccaa aaaaatgtta
     1441 agttcaaaaa cttggctttt gcgattattg acgaacaaca ccgcttcgga gtcatgcaac
     1501 gagccaagct cgtacgcaga tctaatgcag ataaaacgca gattaacgcg gataacagct
     1561 ttctatatca agatttaact tatagagtta ggagtgctat ttttaacgta gctaataatc
     1621 tcggcttagg acacaaggaa ctaatatatc agaacgcttt aacggaagaa ttcaagaaac
     1681 aaaacattcc cttcatcaga gaaaaacaag ttccaatatt ttacttaggc aaaaaaattg
     1741 gagtttacat accagacttc gtaattgata ataaaattat agttgaattg aaagctttac
     1801 cttttttggg taatagcgag aaaaaacaaa catggaacta tttaaaaggt tcagaattta
     1861 aacttgccct acttgtcaac ttcggaccac aaaaacttga aatagaaaga ataatttatg
     1921 acaaagccag gcaaccatct gcgttttatc cgcgagaatc tgcgtctaat cagcatcagg
     1981 tggtgccgca tttgttgtct atgaccgcga cccctattcc tcgcactctg gctctcacaa
     2041 tttatggcga ccttgatttg actttgcttg atgagttgcc cgctggacgc aagccaattg
     2101 tcaccaaaat aattcctccc aacaaaagag aggccgccta tgaacatgtg cgcgaagaac
     2161 tggcggccgg tcgacaagct tatgttattt gcccacgtat tgacgagcca gaccccgaaa
     2221 aagaattggc gatccaagcc aaatcggcac gggccgaagc caaacgactg caagaaaaaa
     2281 actttcctga gtttgaggtg ggcctgatgc atggcaaatt aaaaccaaaa gacaaagaag
     2341 aaacgatgca agaatttgcc aatggcgaaa ttgatatctt ggtttccact tcggtggtgg
     2401 aggttggtgt taacgtcccc aatgcaacga ttattatgat tgaaggagct gaacgttttg
     2461 gtctggccca actccaccag ctccgaggtc gcgtcttgcg ttctagccac caagcctact
     2521 gttacatctt tagcgatacg acaaacgcca aatcaatgga acgcctaaaa gcgctcgttg
     2581 aaactaaaag cggttttgac ctggctgaaa aagatttggc tttgcgtgga gccggcacaa
     2641 tgagcgg
//
LOCUS       DAOOLO010000043         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_47662_length_2644_cov_5.806103, whole genome shotgun sequence.
ACCESSION   DAOOLO010000043 DAOOLO010000000
VERSION     DAOOLO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47662_length_2644_cov_5.806103"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="PLH96_02755"
     CDS             complement(<1..1487)
                     /locus_tag="PLH96_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPB60705.1"
                     /translation="MNENPKIELIIPDPVLEVAKTLENKGFSAYLVGGCVRDSLMNRS
                     PKDWDITTDAKPEDIVAAFPKTVYENKFGTVTVVNENLGEGESRTLKNIEVTPYRREA
                     TYSDQRHPDEVRFCSKLEDDLSRRDFTINALAYDPFRHELIDLYGGLKDIKDKTIRTV
                     GNPDERFREDALRLLRAIRFAAELGFTLNIETQKSIFNNAELIKKTSLERIQDEFVKI
                     LMSNRPMEGINLMSTSGLLKYIIPELEKGIGMEQGGDHIYDVWEHTLLSVQHSADKGW
                     PLHVRLAALLHDIGKTKTRRPGQKGQKIWTFYGHEVVSERMTKEIMQRLKFPVKLIET
                     VLKLARNHMFFADPDKITLSAVRRIVAKVGPDLIWDLMNLRICDRVGMGRPKEEPYRL
                     RKYESMIEEALRAPTSVGMLKIDGLRLMEVTHETPGPKVGMILNALMEEVLDKPELNT
                     EKYLEKRALEMAQMPEKELKKLAEKGKERKEEVEKQELKKIRGKY"
     gene            complement(2174..2247)
                     /locus_tag="PLH96_02760"
     tRNA            complement(2174..2247)
                     /locus_tag="PLH96_02760"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2213..2215),aa:Gly,seq:gcc)
     gene            complement(2386..2472)
                     /locus_tag="PLH96_02765"
     tRNA            complement(2386..2472)
                     /locus_tag="PLH96_02765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2436..2438),aa:Leu,seq:cag)
BASE COUNT          628 a          649 c          543 g          824 t
ORIGIN      
        1 ctgtacttcc ccctaatttt tttaagttct tgtttctcca cttcttcttt tcgttctttt
       61 cctttttcgg ccaatttttt tagttctttt tccggcattt gagccatttc taacgctctt
      121 ttctctaagt atttttcggt gttcaattct ggtttgtcta ggacttcttc cattagggcg
      181 tttaaaatca tgccaacctt tggccctgga gtttcatgtg taacttccat caggcgtaag
      241 ccgtcaattt ttagcatccc caccgaagtt ggcgcgcgta aagcctcttc aatcatggat
      301 tcatattttc gtagacgata aggctcttct ttaggacgcc ccatcccaac gcggtcgcaa
      361 atacgcaagt tcatcaagtc ccaaatcaaa tctggcccga ccttggcaac aatccggcga
      421 acagctgaca gggtgatttt gtccgggtcg gcaaaaaaca tatgatttct agccaacttt
      481 agaactgtct cgattagttt cacgggaaac tttaatctct gcataatttc cttagtcatc
      541 cgttcgctta ccacctcgtg cccataaaaa gtccagattt tctggccttt ttggcctgga
      601 cggcgggttt tggtcttgcc aatgtcgtgg agcaaagcgg ccagtctcac gtgtaacggc
      661 cagcctttgt cggccgagtg ttgaactgag agcaaagtgt gttcccagac gtcataaata
      721 tggtccccgc cctgttccat tccgatacct ttttccaatt ccggaatgat gtatttcagt
      781 aggccggaag tgctcattaa gttgattcct tccatcggac gatttgacat tagaattttg
      841 acaaattcgt cctgaatccg ttccagggaa gtctttttaa tcaattcagc gttgttaaat
      901 atagattttt gggtctcaat gtttaacgtg aaacctagct cggcggcgaa acggattgcc
      961 cgtaacaaac gcaaagcgtc ttctcggaat cgttcatcag ggttgccaac agttctgatt
     1021 gttttgtcct ttatatcctt tagcccgcca tataggtcaa ttaattcatg gcggaaaggg
     1081 tcataggcca aagcgttaat ggtgaagtcg cggcgtgaaa ggtcatcttc cagtttgctg
     1141 caaaatctta cttcgtccgg gtggcgctgg tctgaatagg ttgcttcgcg acggtatggc
     1201 gtaacctcaa tattttttaa agttctggat tccccttctc ctaagttttc gttaaccacg
     1261 gttacggtcc caaatttatt ttcatagacc gttttgggga aagccgcgac aatgtcttcc
     1321 ggcttggcgt cggtggtgat gtcccaatcc tttggtgagc ggttcatcaa gctatcacga
     1381 acgcaaccac caaccaaata cgctgaaaaa cccttatttt ctaaggtttt tgccacttcc
     1441 aggactggat ctgggattat gagttcaatt ttggggtttt cgttcatttc taaaacctaa
     1501 attaggtcaa atacgtgaag gactttggtt ttaatgggct atcgctcata ctaatattgt
     1561 attgtcgtga caaaagcaag ttatcttatg atgatataac tttttttgta aaatttctag
     1621 aatttggaag taccactatc tgttaagtaa ctgaacaagc cggttatcag gtaacacaaa
     1681 aaataaaagt gcgggcaccc acacctattt tttcgtgacg gaagcaaact attttataat
     1741 gaggtaattt atactttata aagctttcca aaatttaaag gtagtactgt ccgtcagata
     1801 accggttatc tgacaacgag aaaataggtg tgggtgcggg tagggagata tctccctcgc
     1861 ctctcgcctc gtcagccact cggctcgggc tggtcaccgg ctcgcgtttc gctctgctga
     1921 gcaaaatatg ctccgccgtt cgattctccc ccgcaagtga ataaattcac ttgcttaccc
     1981 gcgcccaaaa gcctctgctt tttggtgcgg gtagggagat atctccctcg cctctcgcct
     2041 cgtcagccac tcggctcggg ctggtcaccg gctcgcgttt cgctctgctg agcaaaatat
     2101 gctccgccgt tcgattctcc cccgcaagtg aataaattca cttgcttacc cgcgcccaaa
     2161 agcctctgct ttttggtgcg ggtagggaga atcgaactcc cgtcttaaca ttggcaatgt
     2221 catattctac cactaaacta tacccgctaa atatttaatt tggcataaat attttaacag
     2281 aacctgaaac ttttacaata tcaaacatta aaacaactga ttatttatcc actttaaatc
     2341 ccctcggagc tttattggct tcttttccag ccccaatttc tgtattggtg cccgggagag
     2401 gacttgaacc tccacctcct tgcgaagaca gcgctctgaa cgctgcgtgt ctaccaattc
     2461 caccacccgg gcatttttaa tttgttttcc ttgcaaaagc aaataaattt gctttcttaa
     2521 ggtgcaatta tatgacaaag tccgaaccta ttttgagcga aactgactcg cttcgccccg
     2581 ccgccgctcg ctgacgctcg ccagccggca aaaaagtttt cttcgctttt cctgatttgc
     2641 gcgc
//
LOCUS       DAOOLO010000044         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_52443_length_2470_cov_5.054658, whole genome shotgun sequence.
ACCESSION   DAOOLO010000044 DAOOLO010000000
VERSION     DAOOLO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52443_length_2470_cov_5.054658"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(437..1378)
                     /locus_tag="PLH96_02770"
     CDS             complement(437..1378)
                     /locus_tag="PLH96_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB60706.1"
                     /translation="MTDVQKLKQEFLEYLEIEKGRSLKTVENYDRYLTRFFGQADIKK
                     PLDITDEAIRAYRLWLNRQSSGQTGQNAKKAVRGLGGDTLKKNTQNYYLIALRGFLKY
                     LARRGIPSLAPERIELAKAPARELDLMTEVELGRLLKAPEGNDEKALRDRAILALLFS
                     TGLRVSELCSLSRDSIDLSRDEFSVRGKGEKVRLVFLSDEARQALKNYLDNRKDLGEA
                     MFTSMSKINQKTEQLTPRSVERIIKKYAVKAGIPKKVTPHTLRHSFATDLLQNGADIR
                     SVQMMLGHANVSTTQIYTHVTDKHLRDVHKKFHGKKS"
     gene            1588..1839
                     /gene="rpsO"
                     /locus_tag="PLH96_02775"
     CDS             1588..1839
                     /gene="rpsO"
                     /locus_tag="PLH96_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB60707.1"
                     /translation="MITKKKKETIIKKTRKHDKDTGSSEVQVSLLTERIAELAAHLKK
                     HPKDSHSRRGLLKLVADRRSHLKYLENKKKKAEAVIAAE"
     gene            2018..>2470
                     /locus_tag="PLH96_02780"
     CDS             2018..>2470
                     /locus_tag="PLH96_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPB60708.1"
                     /translation="MSIKHKDQRIGIFIDTQNLYHSAKNLYGGARVNFNAVMKDAVAG
                     RQLIRALAYMVTTEAGDERAFIDALGKIGIEVKSKELQIFVDGSKKADWDVGLAVDAI
                     KSAPKLDTVIIGSGDGDFVPLVQYLKENMGVQVEIIAFGKSSSAKLREA"
BASE COUNT          669 a          547 c          525 g          729 t
ORIGIN      
        1 gacatggttg ttgaaaaggg gaatagacaa caacccaaac gtaaaaaata atgggccgag
       61 gaaaactcta tgaaagaacg aacgaagaac tattgacgtc accacttggt ggaacgttgg
      121 cttttattac ttttggctgt cctttagtgg attcagccta tgccaagacc aaatcatgtc
      181 cgatataatt atagggaaat gtgtccttta aggcaatggg acaaaggttg gtaaagaggg
      241 gataaatgaa agttaagtta taagtcaaaa gttagaagtt agaagtttag attagaaagt
      301 aattaagccc atactttatc attatttgag actattacat aaacttattg tattggtttt
      361 ttcctcaaaa acttaacttg cttttaacct taacttttgg cttttctgcc ccaggcagat
      421 aaatgacttc taacttttaa cttttcttcc cgtggaactt cttatgcaca tcacgtaaat
      481 gcttgtccgt cacgtgggta taaatttgcg tagttgacac attggcgtga ccaagcatca
      541 tctggaccga ccggatatca gcgccatttt gcaaaaggtc ggtcgcgaaa ctgtgccgta
      601 aagtatgagg cgttactttc ttgggaatgc ctgcttttac ggcgtatttt ttgatgatcc
      661 gctccacgct ccgcggggtt aattgctcgg ttttttggtt aatctttgac atgctggtaa
      721 acatggcctc ccctaagtct ttgcgattgt ccagataatt tttaagcgct tgccgcgcct
      781 catcagacaa aaaaactagg cgcacttttt cgcccttgcc acgcaccgaa aattcgtccc
      841 gtgacaagtc aatactgtcg cgcgacagcg agcaaagttc cgaaacacgc aagccggtag
      901 aaaaaaggag agccaaaatt gccctatccc gcaaggcttt ttcgtcattt ccctccggcg
      961 ccttaagcag acgccccaat tccacctcgg tcatcaaatc aagttcgcgc gccggcgctt
     1021 tggccaactc aatccgttcc ggcgccaaag aggggatacc gcgacgcgcc agatatttga
     1081 gaaaaccgcg caaagcaatc aagtaataat tttgggtatt ctttttgaga gtatcgccac
     1141 ccaaaccccg gactgctttt ttggcgtttt gcccggtttg gcccgatgac tgacgattaa
     1201 gccacagacg gtaagcgcga atagcctcat cagtgatgtc caagggcttt ttgatatccg
     1261 cctgaccaaa aaagcgagtt agataacggt cgtaattctc caccgtcttg agcgaccgcc
     1321 ctttttcaat ctccaaatat tccaaaaatt cttgttttag tttttgaacg tcagtcatgc
     1381 ttatattata acagaaacat tttccgacat ctctctcctg gctacttctg ttcttaacaa
     1441 tcctcaaaat ccgacaaggg ggccccatct aacccatgtt gtgggttgtt ttttatatta
     1501 gaactaatca aaaagcgtta ttttgtcatc ttgacgagag aaaggctcat cttgcattat
     1561 tttccaccac gtgctatcat acaaactatg ataaccaaga aaaagaaaga aactattatt
     1621 aaaaagactc gcaaacacga caaggacacg ggttcttcag aggtccaagt ttctttgttg
     1681 accgaacgca tcgccgaatt ggccgctcat ttgaaaaaac accctaagga ttcccattcc
     1741 cgtcgcggtc ttttgaagtt ggtggctgac cgccgctctc atttgaaata cttggagaac
     1801 aagaaaaaga aggccgaagc ggttatcgcg gctgagtaat ttcttaattt ttcttttttt
     1861 ttaattttct tggccaatca ttggattgtt attttattaa tcaggtcgtt ttttaatact
     1921 ttttgaaagt tgaagggcct aagttttggt ttttataatc gcttttgctt atttcttaaa
     1981 cttttgactt ttgccctcta acttttagtt tattgttatg tctatcaaac acaaggacca
     2041 gcgtattggg atttttattg atacgcaaaa cctttatcat agcgccaaaa acctttatgg
     2101 tggtgcccga gttaatttca acgccgtgat gaaagacgcc gtggccggcc gtcagctcat
     2161 tcgcgccttg gcctatatgg tcaccactga ggcaggggat gagcgcgcct tcattgacgc
     2221 cttgggcaaa attggcattg aagtcaagtc caaagaacta caaatttttg ttgacggttc
     2281 caaaaaagcc gactgggatg tcggtttggc ggtggacgcc attaagtccg cgcctaaatt
     2341 agatactgtt attatcggtt ccggtgatgg cgacttcgtg cccttggtcc aatatctgaa
     2401 agaaaatatg ggcgtccagg tggaaattat cgcttttggc aaaagctctt ccgccaagtt
     2461 gcgcgaagcg
//
LOCUS       DAOOLO010000045         2316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_57564_length_2316_cov_4.794339, whole genome shotgun sequence.
ACCESSION   DAOOLO010000045 DAOOLO010000000
VERSION     DAOOLO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57564_length_2316_cov_4.794339"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PLH96_02785"
     CDS             complement(<1..1356)
                     /locus_tag="PLH96_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60709.1"
                     /translation="MEKHPLHLKNPELQKSPEVERAVKRQEKKQHQKIPNDPTVRLET
                     YVNRLENIFLHPNENTRRRLVERYRPQIYDALIIKPENFPESYFELQKRVAREQGYGD
                     VEITPEQREQMIATVREDQKYSLDAWIDYLTSEDAMYPAWFKYYAWNQVIKLSQFDKE
                     RGEFKKRTDTTVAPFPDIYREPLAQIADRYKRVKDNNKDAKAREDFDKKFPSLYAELT
                     SKSLAASMENRGETKGQWIKYEQNKTGEAEKLFASLQGKGTGWCTAGQSTAQTQIESG
                     DFYVYYTNDIEGQPTQPRLAIRMEGANRIGEVRGVLQGQNVEPTMQEVLDKKLEDFGT
                     EADVYRQKSADMKKLTELEHRREKGEEFTKEDLAFLYEIDHKIEGFGYEKDPRIEELK
                     AGRDIKNDFVLITGYQAEEISLTKEEALSGGIKYHYGDLYLDSLTSAEGLNLPQTIGG
                     NL"
     gene            complement(1382..2029)
                     /locus_tag="PLH96_02790"
     CDS             complement(1382..2029)
                     /locus_tag="PLH96_02790"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPB60710.1"
                     /translation="MAKLILQRHLLSKWNKENKFTGWQDVPLSEEGLAQAPMVASKLP
                     VKPDEVYTSPLVRNKETARLIAENISVRPVFLASEDSTEVGENELSIYLTKALDERCY
                     GKLEGKNKDEVKKEFGEEQVHLWRRSWDVAPPGGESLEDVYNRALAFFQNKIEPKLRQ
                     DKNILIVGSHNSLRALAKYLEKIPDDVIADYEIPFGGMIVYDLDDNLSVVKKEVK"
     gene            complement(2101..>2316)
                     /locus_tag="PLH96_02795"
     CDS             complement(2101..>2316)
                     /locus_tag="PLH96_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60711.1"
                     /translation="SDWVSKTIQNMLGERKFSRICICKTNGLRGQKLEDSWLEVASGI
                     LMSTLDDGYVLRDSQGRASVKSPIQIR"
BASE COUNT          539 a          534 c          493 g          750 t
ORIGIN      
        1 aagattgccg ccgatggttt ggggcaagtt gaggccttcg gcggaagtga ggctatcaag
       61 gtaaagatcg ccataatgat attttatacc gccactcaaa gcttcttctt tggtcaagct
      121 gatttcttcg gcttgataac cagtaattag gacaaagtca tttttgatat cacggccggc
      181 ttttaactct tctattctag ggtctttctc ataaccaaaa ccttcaattt tgtgatcaat
      241 ttcatagaga aaagccaagt cctctttggt gaattcttcg cctttttctc ttctgtgttc
      301 caattcggtc agctttttca tatcggcact tttctgacga tagacatcgg cttcggtccc
      361 aaaatcttcc aattttttgt caagaacttc ttgcattgtt ggttctacat tttgcccttg
      421 cagaacacca cggacttcac caatgcggtt cgcgccctcc attcggatcg ccagacgagg
      481 ttgggttggc tggccctcta tgtcattggt gtaatagacg tagaagtcgc ctgattcgat
      541 ttgcgtttgg gcggttgatt ggccggccgt gcaccagccg gtacctttgc cttgaagaga
      601 ggcaaaaagt ttttccgcct ccccagtttt attttgttca tatttgatcc attgtccttt
      661 ggtttccccc cgattctcca tggaagcggc cagagatttg gaagtgagct cggcatagag
      721 cgaggggaac tttttatcaa aatcctctcg ggctttggca tctttgttgt tatctttgac
      781 acgcttgtag cggtcggcta tttgagccaa aggttcacga tagatatcgg gaaaaggcgc
      841 aacggtggtg tcggtgcgtt ttttgaattc tccccgctct ttgtcaaact gcgagagttt
      901 gataacttga ttccaggcgt aatacttaaa ccaagccgga tacattgcgt cttctgaagt
      961 taaatagtca atccaggcat caagagagta tttctgatct tctctgacag tggcaatcat
     1021 ttgttcacgc tgttccggtg taatttcaac gtcaccataa ccttgttcgc gggcaacccg
     1081 tttttgcagt tcaaaatatg attcgggaaa attttccggc ttaatgatca gtgcgtcata
     1141 gatttggggg cgatatcgct ccactagtcg ccgacgagta ttttcgttag ggtggaggaa
     1201 aatattttct agacgattaa cataggtttc cagacgaacc gtggggtcgt tgggtatttt
     1261 ctggtgctgt ttcttttctt gacgtttgac cgcccgttct acttcgggtg atttttggag
     1321 ttcggggttt ttgagatgaa gagggtgttt ttccatatta ttttaattat tattttaatt
     1381 tttatttgac ttcttttttt accaccgaca gattatcatc caaatcataa acaatcattc
     1441 cgccaaaggg aatttcataa tcggcaataa cgtcatcggg aattttttcc aaatatttcg
     1501 ccaaagcacg caagctgttg tgactaccaa caatcaaaat atttttatcc tgtcttaatt
     1561 ttggttcaat tttattttga aaaaaagcta gtgcgcgatt ataaacatcc tctaagcttt
     1621 ctccgcctgg tggcgccaca tcccaactcc gccgccaaag atgaacttgt tcttcgccaa
     1681 attctttttt aacttcatct ttgtttttgc cttccagttt gccgtaacat ctttcatcaa
     1741 gcgctttggt caggtaaatc gacaattcat tctcccccac ttcagtgcta tcttcagacg
     1801 ccagaaaaac tggccgaaca gagatatttt cggcaatcaa acgagcggtc tctttattcc
     1861 gcacaagcgg tgaagtataa acttcgtcag gcttcactgg taacttgctc gccaccatcg
     1921 gtgcctgggc caatccttct tccgataacg gcacgtcttg ccagccggta aacttgtttt
     1981 ctttattcca cttggataat aggtgtcttt gcaaaattaa tttagccatt gggcccatta
     2041 taccaatccc tcgcccacaa aaaaagcccc cggctggcgg aggcattgtg gctgtttggg
     2101 ttatctgatt tggatcgggc ttttgaccga tgcccgtcct tggctgtccc gcaagacata
     2161 accatcgtca agagtggaca ttaggattcc actggcaact tcaagccaag agtcttcgag
     2221 tttctgacct cgaagaccat tggttttaca gatgcaaatg cgggaaaact ttcgttctcc
     2281 caacatgttt tggatagtct tgctgaccca atcact
//
LOCUS       DAOOLO010000046         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_63677_length_2162_cov_7.222117, whole genome shotgun sequence.
ACCESSION   DAOOLO010000046 DAOOLO010000000
VERSION     DAOOLO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63677_length_2162_cov_7.222117"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLH96_02800"
     CDS             complement(<1..269)
                     /locus_tag="PLH96_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60712.1"
                     /translation="MENKSYAVPVIVTIAIIILAGAFFFYQGPSIDNGQTASIIQSLA
                     PLLQPAEDVNMDSLRAVSGDDLLRGEAAAPIKLVIYSDLECPACA"
     gene            complement(666..992)
                     /gene="metG"
                     /locus_tag="PLH96_02805"
     CDS             complement(666..992)
                     /gene="metG"
                     /locus_tag="PLH96_02805"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPB60713.1"
                     /translation="MISFEEFKKVELRVAEILAAGRVEGSDKLLKLKASLGEDERQII
                     AGIGKNYEPEGLVGKKVIIVANLEPRSLLGLESQGMILATSNEEGRSTILTPESDNVA
                     PGTLIT"
     gene            complement(979..1578)
                     /locus_tag="PLH96_02810"
     CDS             complement(979..1578)
                     /locus_tag="PLH96_02810"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB60714.1"
                     /translation="MPRIKELVNTLKKGEVVIMPADTIYGLFGLARNSQTVKKIAKLK
                     GRHFDKPFIVLVASWAQLDELGIKITEREKRFLKNVWPGKVSVVLPDKNSRFEHLHHN
                     RGLAVRWPKHKFLTTLLKKTGPLVSTSANKADNPPAETITEARVIFGNQIDLYLSAGR
                     RLKNKPSTLISLENGKIKVLRKGALPIKKLKQKLNYDLV"
     gene            1698..2126
                     /locus_tag="PLH96_02815"
     CDS             1698..2126
                     /locus_tag="PLH96_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB60715.1"
                     /translation="MLSDYYQIELKDFDQDFFDLLETVGQIKQPDFKVLPYFHTVYAN
                     GEKAGIVGYQPASLPDTGNVEIILAPEYRGKGLIAKIYDTLAQEHHLKTLYATINLRN
                     MTSLSAHEKIGFQRLPAEKQAQLKEKGIIGEGEMRLEKSV"
BASE COUNT          592 a          445 c          495 g          630 t
ORIGIN      
        1 caggcgcaag ccgggcactc caagtccgaa tatatcacta gttttatggg tgcggctgcc
       61 tctccccgca ataagtcatc tccactcacc gcgcgcaaag aatccatgtt tacatcttct
      121 gccggttgga gcaggggcgc caagctttgg atgattgacg ctgtttgacc gttgtcaatt
      181 gacggacctt gatagaagaa aaaagcgccg gcaagaatga tgattgcaat agtgactatc
      241 accggcacag catatgattt gttttccata gaataaggct atttataagg tttttaataa
      301 tatgtcgtca ttatacactt ttttagcaat ttgggaactg gtcaaaatcc agcagaagtg
      361 gtactggtaa ttggctccgg attggagctg gttgcttggt ttgttgtgtt tgtggaagca
      421 agcgtagagg atgatgtgct agacgcggat agtatttttt caaaatgtga taagtcttca
      481 ataaaaattt ctcctgtttc attttggcat tgtctgggca aagtttccca aacggggaag
      541 ccgccagcca cacaactgct aaaatcggtt ggctgattgt ttcgtgggta aaacgaccag
      601 gcaataaaaa caaaaagcag aaaagaaacg gccgtaaata tgctggtttt ggttcgcgaa
      661 gtcatttagg taattaatgt tccgggcgcg acgttgtcgc tttccggtgt caaaattgtt
      721 gaacgacctt cctcgttgct ggtcgccaag atcattcctt ggctctctag tcccagcaaa
      781 gaacggggtt ccaaattggc gacgataatc actttttttc cgaccaaacc ctctggttca
      841 taatttttgc caatcccggc aataatctgt ctttcgtctt cacctaggga agcttttagt
      901 tttaacagct tatccgagcc ttctactcgg ccggcggcca aaatctcggc caccctaagt
      961 tctacttttt taaactcttc aaacgagatc ataattaagt ttttgcttaa gctttttaat
     1021 tggcaaggcg ccctttctta acacttttat tttaccattt tctaacgaaa taagagtgga
     1081 gggtttattt tttaggcggc ggccggcgga taaataaagg tcaatttggt taccaaaaat
     1141 gacgcgcgct tcggtgattg tctcggccgg agggttatca gctttgtttg cgctggtaga
     1201 gacaagaggc ccggtctttt tgagcaaggt ggtcaaaaac ttatgcttgg gccaacgcac
     1261 cgccagcccg cgattgtggt ggagatgctc aaaacgggaa tttttatcag gcagaacaac
     1321 actgactttg ccgggccaaa cattcttcaa gaaccttttt tctcgctcgg tgattttgat
     1381 tcctagttcg tccagttgcg cccagctagc cactaaaaca ataaacggct tatcaaagtg
     1441 gcggcctttc agcttggcta tttttttgac tgtttgactg ttgcgagcca aaccaaaaag
     1501 accataaata gtgtcggccg gcattatcac aacctcacct tttttgaggg tatttaccaa
     1561 ctcttttatt cttggcatat cagtgttaaa attagcataa ataagcggtg aaagccaacg
     1621 aagagaagct cgggtttcag gcgtcagctt ttgacaataa tgggcatttt taatagctta
     1681 gctggtaacc aatttttatg ttaagcgact attaccaaat tgaattaaaa gattttgacc
     1741 aggatttttt tgatttgcta gaaacagttg gacaaataaa acagcccgac tttaaggttt
     1801 tgccttattt tcatacagtc tacgccaatg gtgaaaaggc ggggattgtc ggttaccaac
     1861 cggctagttt gccggacacc ggcaatgtgg aaattatctt agcgccggaa tatcgcggca
     1921 aaggtttgat tgccaaaatt tatgacacat tggcgcaaga acatcatcta aaaactttat
     1981 acgcgacaat taatctgcgt aatatgacat ctctctcggc ccacgaaaaa attggttttc
     2041 aaaggttgcc ggcggaaaaa caggctcagc ttaaagaaaa gggaataatc ggcgaagggg
     2101 aaatgaggtt agaaaaaagt gtttaaagcg aggctaattt ctctgcaagt aaactttggt
     2161 gg
//
LOCUS       DAOOLO010000047         1860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_79422_length_1860_cov_4.800554, whole genome shotgun sequence.
ACCESSION   DAOOLO010000047 DAOOLO010000000
VERSION     DAOOLO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79422_length_1860_cov_4.800554"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..860)
                     /locus_tag="PLH96_02820"
     CDS             complement(348..860)
                     /locus_tag="PLH96_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB60716.1"
                     /translation="MQKHVLTGGPGIGKTTLIELLAARDFEIVPEAARMVIEEEKAKN
                     SDVLPWKDLGLFQTRVAELQLELEEKNKGEIIFLDRSLIDGYGYCVLEKVPPPTIIEQ
                     LGRNRYDKIFLLDPLPNYENDHSRLEEKTKAEAIHQAINQAYLDFGYHPLKVPILPPA
                     ERVEFILKNI"
     gene            complement(1016..1087)
                     /locus_tag="PLH96_02825"
     tRNA            complement(1016..1087)
                     /locus_tag="PLH96_02825"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1052..1054),aa:Glu,seq:ttc)
BASE COUNT          473 a          373 c          437 g          577 t
ORIGIN      
        1 tttgaaccag aggtggggtc gggaggactg ggtgtcctcc cgtggaggaa aacagcgagt
       61 ctgcgagtgt tagaaaccgt gggtatctaa agcgcagacg agtcctcgaa gtcgagcagt
      121 gaggttccca ccctttccgc cttcgcctcg ctgtagttcg gcttcggcgt gacgcagtcc
      181 gtcaagacga gcgaaggtgg gaagaaaatt tatgcattac gtcacagtta ttccaaccaa
      241 gagcttcttg actctgtcga gaagattccg cggttggtta actaattaag ttctaaggtc
      301 ataaaattca agcgcctgac cagcgctttt ctttttaata aaatagttta aatatttttc
      361 aaaataaact ccaccctttc tgctggtggt aaaatcggaa cttttagcgg atgataaccg
      421 aagtctaaat aagcttgatt gatagcctga tggattgctt ctgcttttgt tttctcttct
      481 agccgtgaat gatcattttc gtaattaggt aggggatcga ggagaaaaat tttatcataa
      541 cgatttcgcc ctaattgttc aataatagtc ggtggtggca ccttttccaa aacgcaataa
      601 ccgtaaccgt ctatcaggct gcggtccaaa aaaataattt cacctttgtt tttttcttct
      661 aattccagtt ggagttcagc gacgcgtgtt tgaaacagtc caagatcttt ccagggtaaa
      721 acatcgctgt ttttggcttt ttcttcttca attaccattc ttgccgcttc tggcacaatc
      781 tcaaaatcac gcgccgccaa gagttcaatc aaggttgttt tgccgatgcc ggggccgccg
      841 gtgaggacgt gtttttgcat tagctgtatt ttaaaagata ttttgctttt agacaattag
      901 aggggttaga ggcgcttcgc gctcaagatc gttgtaatcc actctggcgg gcagtttgct
      961 ctcttagggt cgcgaataaa aatggccagc ttgcgccggc tcatttttgt actcgcgacc
     1021 ctaccgggaa tcgaacccgg attaccacct tgaaagagtg gtgtcctaac cgttagacga
     1081 tagggccaat tttaaattta acaaactttg tatagaaaaa taggcccgag gccgtttttc
     1141 tacgttgtct aatatactat aaattgacaa tttgggcaac acttgtcgtc aagaattaat
     1201 ctcgttatac ttaggctgga tccgtttttg aaccaaagga ggtgagagat gaagaatctt
     1261 tcatatctga aagttctcaa caaagaacaa gcccttcaga ttcttgggca agaatatccc
     1321 gacacagaat tttcttttgt gcatcgggtg caccataata attgtgacgg gatgacatac
     1381 tgtttcagga gcccatcgca acatcacgtt tactattacg tggatgagaa cgatggctcg
     1441 gtgagagtcc aggatttgag ggcccaaaag gcgagctcct agctcgcctt ttatttttta
     1501 attgacaaaa cttgccagga gccttgggga gagaattaca ctttttgcgt tgttacgtaa
     1561 catgttacgt aacagttaaa acgttagggg aggacaatct ttttccaact tggcgcgagt
     1621 ttaagggata gtttataata aatagtataa atcagcgcgc ttgtcgcttt aaaaataatt
     1681 ggattatgac ctatttggta gctattttgg cttttgtctt aatgttagcg ggcggatttt
     1741 ttgcgctgcg ttttcgtgac cgcctgcatt tggttttggg cttttcggcg ggcgctttga
     1801 ttggagttgc tttttttgac cttttgccgg aagcgataga gatgactgga aactttttgt
//
LOCUS       DAOOLO010000048         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2699
            NODE_82329_length_1817_cov_4.435301, whole genome shotgun sequence.
ACCESSION   DAOOLO010000048 DAOOLO010000000
VERSION     DAOOLO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864774
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 571
            CDSs (total)                      :: 529
            Genes (coding)                    :: 527
            CDSs (with protein)               :: 527
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82329_length_1817_cov_4.435301"
                     /isolate="AMR_MDS_2699"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLH96_02830"
     CDS             complement(<1..381)
                     /locus_tag="PLH96_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002166369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPB60717.1"
                     /translation="MNISVGIVGLPNVGKSTLFNALTRKGVPAENYPFCTIDPSVGIV
                     PVPDERVGLLADFSKSAKQIPAVIEFVDIAGLVKGASAGEGLGNQFLQHIREVDAIAE
                     VVRVYEDSNIAHVSGRIDPIDDIET"
     gene            complement(385..675)
                     /locus_tag="PLH96_02835"
     CDS             complement(385..675)
                     /locus_tag="PLH96_02835"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPB60718.1"
                     /translation="MALVSDFRTKVLDVVSRIKVGETLSYGEVARRAGSPKAARAVGN
                     ILNKNFNSQIPCHRVIRADGQVGGYNRGVKAKIRRLKQEGVKLVFGLTSAKV"
     gene            complement(681..917)
                     /locus_tag="PLH96_02840"
     CDS             complement(681..917)
                     /locus_tag="PLH96_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60719.1"
                     /translation="MVHMFKEFFVKKMLQSKGVPKEQIEPMMAIIKKNPAFFQQIAGE
                     IEEKVKKNKVDHMTATMEIINNHRDELQKLLTDK"
     gene            complement(910..982)
                     /locus_tag="PLH96_02845"
     tRNA            complement(910..982)
                     /locus_tag="PLH96_02845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(947..949),aa:Ala,seq:ggc)
     gene            1267..1476
                     /locus_tag="PLH96_02850"
     CDS             1267..1476
                     /locus_tag="PLH96_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB60720.1"
                     /translation="MDSKIVTTIIVLIIIVVLGIIIYNYYVAEPNDIDVVPDTTTSGD
                     VFDDFDNEDIFGTTSATTSETDLLE"
     gene            1614..1690
                     /locus_tag="PLH96_02855"
     tRNA            1614..1690
                     /locus_tag="PLH96_02855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1648..1650,aa:Ala,seq:tgc)
BASE COUNT          517 a          433 c          355 g          512 t
ORIGIN      
        1 cgtttcaata tcatcaatcg ggtcaatccg accggaaacg tgagcaatat tggaatcttc
       61 ataaacgcgc accacttcgg caatggcatc cacttcgcgg atatgttgca aaaattgatt
      121 gcccaaacct tcaccagccg acgcgccttt gaccaaacca gcaatatcca caaactcaat
      181 caccgctgga atttgtttgg ctgacttaga aaaatccgcc aacaaaccga ctcgctcatc
      241 aggtaccggc acgatcccaa ccgatgggtc aattgtgcaa aaagggtaat tttcggccgg
      301 cacgcctttt ctggtgagag cgttaaaaag cgtggacttc cccacgttag gcaaaccaac
      361 aataccaaca gaaatattca tagcttaaac tttagcacta gttaagccaa aaaccaactt
      421 gacgccttct tgcttgagcc gacgaatttt ggctttgacg ccgcggttat aaccgccgac
      481 ctgaccgtcc gcgcgaatga cccgatgaca aggaatttgc gaattaaaat ttttattcaa
      541 tatgttgccc acggcgcgcg cggccttggg cgaaccggcg cgccgcgcca cttcaccata
      601 agaaagcgtc tcgccaactt taattcgcga aacaacgtcc aaaacttttg ttctaaagtc
      661 ggacaccaag gccatctttg ttatttgtcc gttagcagtt tttgcaattc gtcgcggtga
      721 ttattgatga tttccattgt cgccgtcata tggtcaacct tatttttttt gactttttct
      781 tcaatttcac cagcaatttg ttgaaaaaaa gccggatttt tcttgataat cgccatcatg
      841 ggctcgattt gttctttagg caccccctta gattgcaaca tttttttaac aaaaaattct
      901 ttaaacatgt ggaccatacc ggaatcgaac cggtcacctc tcccatgcca tgggagcgct
      961 ctaccaaatg agctaatggc ccttgaccag aaaactcttg cccgcgacac catagtccaa
     1021 ttttagacta ttttcaaatg taaatataac aaatctttta taaattgtaa aagagttaaa
     1081 aagttttacg cgcaaaaaaa acaaatcaag atatgtttgc caacttttca atagaacctc
     1141 ctggctgagc gctttaataa aagtagttca gccgatttta atagaaactt cataaaattc
     1201 atgattaatt aaaacagcaa taaatttagt cgtttttaaa attatcagca atttattttg
     1261 taaaatatgg actcaaaaat agttacaaca atcattgttt tgataatcat tgttgttttg
     1321 ggcataatta tctacaatta ttatgtcgct gaacctaatg atattgatgt cgttccggac
     1381 acgacaacaa gcggcgatgt ctttgatgat tttgacaatg aggacatctt tggaacgacc
     1441 agcgccacca cttcggagac agacttgctt gaatagaaat aacctaaaaa ccgacaacct
     1501 ccctccgtgg gaggctgtcg gtttttagct gacaggtatt ccagcctgtc ctagtttgtg
     1561 ttatattaat tttgtttacc caaagtatac gcttgtgtat attttaatat tcagggcccg
     1621 tagttcagtt ggctagaacg ccacatttgc aatgtggagg tcaccggttc gagcccggtc
     1681 gggtccacca ttctgaattt gaagaaagat tgacctcgcc cgcgccaaag gcgcgggctc
     1741 gggctggtat ttccgccaag tcccgccaag gcattttgaa ttccgccgag agcattcccg
     1801 ccgctacgcg gcggttc
//
