LOCUS       DAOOLY010000001        30694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_1279_length_30694_cov_4.819935, whole genome shotgun sequence.
ACCESSION   DAOOLY010000001 DAOOLY010000000
VERSION     DAOOLY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 30694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30694
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_30694_cov_4.819935"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLD15_00005"
     CDS             complement(<1..817)
                     /locus_tag="PLD15_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB62305.1"
                     /translation="MEKQKSAIARYWKQYKWSNIFAMIKNNGANPEICGDDFKDRLMV
                     ITGGTSGIGYYTCRKYASHGARILSINRNGEKSERLCDELKRDFGVECSYMIADFSSL
                     KDSLSVGEELSAMKEPIDVLIHNAGVFLKRRELTGDGFETTFVVNHLSSFVINYLIKD
                     KLKSQEKARVLMVNSEGHRFAVWGIRLDDLNWQKRRYSGLKAYGSAKTAQLLGMIIFN
                     EYFEGSGVTMNAMHPGAVRTGTGKENGAFYRWYKRTIVDRLSRSPEISAEALYY"
     gene            complement(827..1270)
                     /locus_tag="PLD15_00010"
     CDS             complement(827..1270)
                     /locus_tag="PLD15_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPB62306.1"
                     /translation="MDEFVIESADQIRELWSKTYNTEGKPDWSHILPYYDENILFRDT
                     IQEIRGLEEFKKMTERLAERSKELKMAILRVVMEDRTVFVEWEMTILFKKTRTSVIYG
                     ASRLSLNERGKIVEQRDYYDLWGDIFDNIPAFRKPYRKFMRKHFG"
     gene            complement(1394..3700)
                     /locus_tag="PLD15_00015"
     CDS             complement(1394..3700)
                     /locus_tag="PLD15_00015"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPB62307.1"
                     /translation="MRKQTVFWLLAIFVLISFVSSPARLFGGQIEIVRDYWGVAHVYA
                     ETDSELFFGAGYATAQDRMFQMELSRRKVAGRLAEIYGKDWLESDKLMRTLGLYRHAQ
                     KIADQLQGETKTILEAYAGGVNYYLETHLDDLNPAFYEAGITPEPWTVADSIAVWMRI
                     SERFDRSWQNEVTALRSYEQRLRSGVTQDPVVIDDFAAIVTEADFISTDPDTYRRLME
                     NEKLDELSAWLEWDSLKASHAWTISGSKSLTGSPLLESDPQIAVTLPSLWYEIHLSGG
                     NYNVRGICVAGSPGFLIGWNEYLAWGATALGSDNSDLFQERLDDDGSSFLFKENWYPV
                     QSYTEVIDIRDEEPVEITVNKTIHGPLVDAFLNGARPAEYFSLKYVATQELKTPITGM
                     LSMMRSRNWEEFTCGLGNYMFPGLHVVYADVSGNIGYYTAAAIPIRSGRPGLPQIGWT
                     GDEEWNGYVPFEELPHVLNPSTGFVASANHLPVGAWYPYSLTIGTGGTGHNPRSMRLY
                     ELLNDQDGFTFTEFSEIHRDNVSAIAREFLILAAILFERNSLSQNSSRFLSAFDGWDY
                     SLIENSRVSDLAETLVQVMHRSLRSNTATALLASKYGGGHAGNIYLLRSVLAEFERSG
                     TLPDEEEVTIWVNQVLETAVAGIRESVSQKRPQVMIYQANLEGFPSIYPDANRYMPYL
                     TATSANTIWSQLGNSYTQVVDLSDIDNSRAFLPPGVSEDPRSPHFFDQVELWAAGETR
                     PAPLSREEVMVYSQSVETLYLYVTETKE"
     gene            3946..5214
                     /gene="murA"
                     /locus_tag="PLD15_00020"
     CDS             3946..5214
                     /gene="murA"
                     /locus_tag="PLD15_00020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB62308.1"
                     /translation="MSQLVVTGNTRLTGEIRASGSKNSVLPILAATVMIEEPVVIKNV
                     PELKDVETMISILQQIGKIVTFYNETVTVKPGEVLIGSVPYELVSKMRASFNVIGPLA
                     MICGWARVGKPGGCNIGQRPVDFHLKGLEAFGFKITEEHGDVLAMIPSEFKKEIIYTL
                     PFPSVGATEQLMTTAALMNGTHVIIENAAREPEITDLQGFLNSCGARVSGASTPTIEV
                     YGVSSLHGGDYSVIPDRIETGTYLLGALTTRGDVTITGARYDHIISLISVLEEMGAGV
                     EKGNDFIRGFWKGPLKPVTVSSEPYPGFPTDLQPLLTSVLTTVEGTSILEENVFENRF
                     GYVDELNRMGARIKVSSRQAFIIGVHRLSGAEVVATDIRAGAALVMAGLAADGETTIN
                     NVTHIFRGYERFQDKLSRLGAKIAYYPDDR"
     gene            5384..6250
                     /locus_tag="PLD15_00025"
     CDS             5384..6250
                     /locus_tag="PLD15_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB62309.1"
                     /translation="MLSCDLCPRNCRVNRYETAGFCRQFADPRVSAIVLHFGEEPPLV
                     KDGGAGAVFFSGCTMSCIYCQNFAFSQKNNGREISAERLGEAFLNVEREGAVCLDLVT
                     PTPNFYGFLRAYEYALVRGFSLPIVFNTSGYEKPDTVKLLEGVVDIYLADIRYTDDQI
                     GKKYSGVADYWSVTRKAIKEMFSQVGSFKDEEMRGVIIRHLVLPENLAGTQEMAEYVA
                     FELSASVPISLMSQYRPVYRAREYPEISRRITQEEYNDALELLDNYGLTGWMQHFDLE
                     EPFRARPVGWDQ"
     gene            6247..6600
                     /locus_tag="PLD15_00030"
     CDS             6247..6600
                     /locus_tag="PLD15_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62310.1"
                     /translation="MIFSISLFLWITFFGKITPAALISGSIVSGFVQYISSKLVPKGP
                     SVRTAFKILLSLPPAIFQSFRLLFSKPVFTVRAEEIPENRIEEFGKIISVTMTPEEIV
                     VSKDREGFLIHEVKR"
     gene            6597..6845
                     /locus_tag="PLD15_00035"
     CDS             6597..6845
                     /locus_tag="PLD15_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62311.1"
                     /translation="MIFYITMFSLMASIAMLVWFSAGQDPWRLLIGYSSISTRLLVGI
                     VFIELVTQVDFISGVALLFLILNTSGTIIAAYYLGVRR"
     gene            6845..7120
                     /locus_tag="PLD15_00040"
     CDS             6845..7120
                     /locus_tag="PLD15_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPB62312.1"
                     /translation="MEIAGCVFLFVGLVFVVVGTLRSVIAHTIVATLHFLTIADTVGL
                     IFIIVSAFFFGCLSIIETIAFVAALMVTGPLVTHVIARAYINSEGER"
     gene            7117..7806
                     /locus_tag="PLD15_00045"
     CDS             7117..7806
                     /locus_tag="PLD15_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPB62313.1"
                     /translation="MNTVVIMIGALYLLSTVIVVASKSSFQAVIWFGIMGSLSAIVML
                     LAGAPDVAMTQFTVGVALVLIVYIMALKRQRRVRLGFVNVPSMIEIGSGGLKGLEWEI
                     MKRIDEKEGYHIEAAAFESRETAIKAVEEHEIDILCGAFTEEELSGSTRGIAYLETSI
                     FDFEGVEVDFVKLKQLSRSSVSPRPVFLKKSNYVFVVSKSSGDLAEALSSDIENMTKE
                     GKMQEIVGRYL"
     gene            7812..8483
                     /locus_tag="PLD15_00050"
     CDS             7812..8483
                     /locus_tag="PLD15_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPB62314.1"
                     /translation="MKILGVIAAAVIFLFVVLQARIDLVFPEGLEEIIERTNIPNAVT
                     AIYLETRLYDTIFEVIVFSITALGVTTLFSSLPRSAEGSQQVFGSVTVYSGGLAALSV
                     TLFLYVVLEGHISPGGGFVGGVVLATGIVTYGLTSNFAKANSHYDRFKIKIMENASLL
                     IIFSLVSFIVFFPETHARLLASGEFGEVLSGGLIPVLNILIGIKVYAGAWKMSSEFIN
                     RRGTL"
     gene            8480..8836
                     /locus_tag="PLD15_00055"
     CDS             8480..8836
                     /locus_tag="PLD15_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HPB62315.1"
                     /translation="MTGNIIFYISVAGMLVGTVGMILSRNIIKKILSLGIVETSINTL
                     YTGISSRKSIIPPIVNGDFSIAYADPVPQAVIITGIVISFALLCLSLVFAIIIYNKYH
                     TMDIGTIESNFEREKE"
     gene            8833..10272
                     /locus_tag="PLD15_00060"
     CDS             8833..10272
                     /locus_tag="PLD15_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPB62316.1"
                     /translation="MIFTIFLPVLGGIICLILRERRRLCAGVALFATIINGSFLPFNL
                     FEEFGMNLGNWGELGIRLTMDDLAIPFMLSTFLVMLAITLNSLRKNYDGFFYSLILIM
                     YGTLNSIFLSRDLFNVFVTVELVSIISFILISYEKKGRQAWASLKYLLLSSLGLNFFL
                     LGIGLVYMETGSFAMEGLNHVSSIASVLIFGGLAVKSGLFLFSMWLPDAHSNAPVEVS
                     PILSGLVVKIDVYLSIRFVQYESFGWLKDEFVYIGIFSAIAGIILAVNSKDAKRLLAY
                     STISQVGFMMVTCDKASSWHGFSHAVFKTLLFLVVGNVSTRLGTKEYRDWSGRITRIE
                     YLFLLVGSLAISGFPMTSGCVTKEVIMHDACCPWLKVLMMLSSVGTAMIFSKFIFLKP
                     SKETGSLPTGTLAAYSILSVAIIVHGIVGFEIYMFESLLMIAAGVGLYFLYRKFMRPL
                     PVFFERIDNSLAIYLIMFLFSIGFVIFST"
     gene            10307..10382
                     /locus_tag="PLD15_00065"
     tRNA            10307..10382
                     /locus_tag="PLD15_00065"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10340..10342,aa:Trp,seq:cca)
     gene            10673..11791
                     /locus_tag="PLD15_00070"
     CDS             10673..11791
                     /locus_tag="PLD15_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62317.1"
                     /translation="MKLNRVLVLAIVLLLGVAALGATIKVALVAPFTGLGSILGDYIK
                     MGAQLALEEINAAGGINGDMLEMIVYDDAANPSTAANVVRRALFQDNVVAVFGPNMSS
                     AVIGVHPLAQQAKIPMLVGATSPSFRYTKIPNDFLFRLRADDEVKVVQLVKYAVEVLG
                     IKKPGVIYGSTDYCLAALEVAKVEFPKYGVEIVAMEQIKEGDKDATGQLLKLKNAGFD
                     GLIGLTHESEAAVTVNQLRQLQIDVPIIGFSAWGVPAFTDLAPEAAIGVYSVQGFNPV
                     DPDPAVVAFVDAYKARWDGKEPSDPAQAYYDGMYLLAEAIKNVGTDGTKIAQYLATKA
                     NIVGVQGELKCDEHHNFTNVCLISQFDGKDWKIINKMF"
     gene            11861..12751
                     /locus_tag="PLD15_00075"
     CDS             11861..12751
                     /locus_tag="PLD15_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB62318.1"
                     /translation="MLQDILQNIVSGASIGATYGLVGLGVVFLWLTISRINFANISSA
                     MLGAYLFYTFYTMVNLGFFLSFVLSVGVIALYGIGLRAFIYEPIRKRGGGRLEFVVAT
                     LMLCTFWLNLIIVTYGALPKPFPPVFGGNKDFITIGSVRIPTLYLNIYLVIAVLMVLI
                     YFILNKTLIGKSFRAAAQNREAAALMGIDVKVTTSLSFIMATCVVGIAGILLAPIYFV
                     SLELGGGSIGVKGFASAVLGGLTNPYGTILGGISLGLLENFSTLYISSTYRDIISFSV
                     LVAVLILKPSGIFNWKSKKI"
     gene            12762..13724
                     /locus_tag="PLD15_00080"
     CDS             12762..13724
                     /locus_tag="PLD15_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB62319.1"
                     /translation="MKKLLFIAGAILVIILFPILNKNMYHLYLMNRALIHAILATGLV
                     FLTGFAGQISLGQAGFYAIGAYSSAYFTVKLGMPIPVGIIIGIAISVLAGFLLSIPSF
                     KLKAFFLSLVTIAFGQIVWMLVINLTPITGGPYGFFGIPFYSVGNNMLSFGQVFWLFG
                     ALLILCVYIMFRIKHSHFGRAMLAMNDDDVATESCGISTKRLKIAAFGFAAGLAGLAG
                     ALYAHLAGFLSPEPFTFFESSNFVAMAVVGGLRHMSGGVVGGIGLTLLPEFLRFGGWE
                     NYYLMVTSLIVIVVIIFMPMGLGPIIENLFRKIFRLKRSNTKTL"
     gene            13737..14495
                     /locus_tag="PLD15_00085"
     CDS             13737..14495
                     /locus_tag="PLD15_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62320.1"
                     /translation="MDILRTENMTKSFGGVVANNNLSIGIPEGKITALIGPNGSGKTT
                     FINVVTGVHPITSGKVFFKDRDISGLDVNVISRMGIARTFQKIRLFENLSVIENVLVA
                     RKPFYKSGPISVVLNTPKFKREEKENKEKALELLKLVGLEDRAYDSPTGMAYGLRRCL
                     EIARALALEPQLLFLDEPAAGMTRDEFKVIMDLMIILKERGITTLLVEHTMDFIKAVA
                     DWVYVLNFGQVIAQGKFEEIERDPLVLKAYLGEE"
     gene            14498..15214
                     /locus_tag="PLD15_00090"
     CDS             14498..15214
                     /locus_tag="PLD15_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62321.1"
                     /translation="MFLLETRDLSVSYNEVPALRKVSIKAAKGEVVSVLGPNGAGKTT
                     LLKSISGLVPCEPGSKVFLNGEEIQNLAPHKITRLGVIHVPEGRQVFAELTVQENLEV
                     AATRVGLAKARPNIERVYELFSELKPRKGQMAGTLSGGEQQMLAIGRAIVSEPLIMMI
                     DEPSMGLAPVIVKRIFETLKKFVKDTGLTMLIVEQNAKLSLPMSDRVYIVSQGQIKLE
                     GSVEEVKSDEKIREMYFGGK"
     gene            15216..16466
                     /locus_tag="PLD15_00095"
     CDS             15216..16466
                     /locus_tag="PLD15_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPB62322.1"
                     /translation="MLVELDYTTGEKLGLEITAGSLLAPLKITTGKGAHRDLESSLST
                     ADNVDLYTMAREAGRIVFIVEDHTRHSPVLGTLGHLKFEFGRRAIPWDRVKLLVATGT
                     HRKMTKIELVEKFASFLASTEIIQHDAADGVEMKDFGNYLDVPLKINRNVESDLLVAI
                     GSIVPHRFSGWSGGAKMVVPGVASYETVFQSHRMAILNSSADVGVVENEFRDLIDETG
                     SRVGLDFIINYYYGIDGSVMGCVSGDHITAHRRGVELARNELLRVFERRSDITVISSY
                     PSITDFWQGGKALYTADLITKDGGDIVMVSPLNEGFGDHPAFAKLLRLKSAEILEELD
                     GMTAQDPLAYVAAYAVKNIMERKRVHIVSHTAYRSMFNEIGLSVHDDLQKTIDSLKAK
                     DREIVVLENSYILPQVETTMEGKL"
     gene            16463..17716
                     /locus_tag="PLD15_00100"
     CDS             16463..17716
                     /locus_tag="PLD15_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HPB62323.1"
                     /translation="MKPKDLDYMPLNGTEYFDGSKWVCPMNFEKAIKGENARESVYIH
                     DVTLRDGEQTCGLTWNEDQRVRIGVALNELGVKSIEVGMPVVSDENKRAIRRLVDMNL
                     TSEIVPFARAMKKDIDASLECGAKRVVVEHAINPYDNEYVYGVSAEKVIDRVVTTIGY
                     AKEQGMVPTFMGWDTTRSTFDYVFKVFTEVARQAKPESIVFVDSFGVASPFTIEFVFT
                     ELRKAIPDIPLEFHVHNEFGLAMGSVFAAVRAGAAGIHSSINGLGERTGNVATEEVAA
                     GLKLLMGIDTGVNLEKIAYTSRLVEDISKIPVANNKPVVGKRLFWVESGIVVDAIDKL
                     NEAGIEPAMTPYLPYLVGMGEIQVKLGAFSGQASIKYWLKRLGITCNDEQLEELTEIV
                     RKEGRIRNTVLEIEEFKKIVSDYLG"
     gene            17713..18762
                     /gene="buk"
                     /locus_tag="PLD15_00105"
     CDS             17713..18762
                     /gene="buk"
                     /locus_tag="PLD15_00105"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPB62324.1"
                     /translation="MSCYILVINPGSTSTKIAVFDGEREVFSKTLTLEENQLDLPLFP
                     EQYLIRKRQILRALADSGIEPNQLSAIAARGGRLRPLSSGVYRINDRMLKDARAGLQG
                     VHPANTAVVIASEIAFENRIEAFTVDPISVDEMSEWAKITGIRDITRNSLSHALNMKA
                     IARRAASQLGKSYEEAKIIVAHLGGGSSVSAHLQGRMVDLYNSDKEGPFAVERAGALP
                     THELADFARSRDDYLHHLAHAGGLYSHFQTRDVEKIVEMARGGDSRAVVVLQAYIYNI
                     AKYIFSLLAVFGGYPDALALTGGVVKSEFVRAALLEKLGKGFTVFLFPGEFEMESLAS
                     GVYRVITGREKALEY"
     gene            18772..19683
                     /locus_tag="PLD15_00110"
     CDS             18772..19683
                     /locus_tag="PLD15_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPB62325.1"
                     /translation="MQINSLSQIVSKARQSGGKVEVLCVQPYDDSTLKALGSLRSWGG
                     GIKLTLFGKREKIVSSEYCRGLEDVEIIEGDTEEINTARAIETIRANKPAVLMKGLVN
                     TAVFLRVLFRSESVKASGRLVSHVSCLEVPGFERLLFITDGTVNVNPDLKTKIGIVEN
                     VCEFVRSLGFEKPKIAVIGINERITQSNQDLIDGAVIAKMSERGHFGNCYIDGPMPLD
                     TALSKEAAVKKMILSPVGGEADVLVCSNLESAANLIKGLVHLAHSKTAGLLLGAGFPV
                     VLTSRSDSSDSKEISLAMAVLNATGGV"
     gene            19690..20520
                     /locus_tag="PLD15_00115"
     CDS             19690..20520
                     /locus_tag="PLD15_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPB62326.1"
                     /translation="MRLAFMIANEDVPSGARVTGLTGSLEENLGQLKKAGFDFFELMI
                     ADPARVDVEKLRFLERKTETGISFLCTGEMAGTMGMALNSPESSQRERALSGLIDAAE
                     KAKALGVNLNIGRLRGTVWEDGPEKSLERLKRALRVVDEYVSNNLPGLSILIEPLRRD
                     ICPLLNSCRETYEFISSSGLKNFAILLDSDHFDQRSDPEYVQEQIGTIKHVHIADTMH
                     EPPGNGKIDFEKFLSLLFKAGYTGDISVEVFCGAEQYETLRDSYRFLGEHSRFWEGLR
                     "
     gene            20517..21245
                     /locus_tag="PLD15_00120"
     CDS             20517..21245
                     /locus_tag="PLD15_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB62327.1"
                     /translation="MTGSDGSRFHGIQQQVYRSILKSIVELEIPPGKKLTIGRLKTIY
                     GVSSTPVRGALYKLKEDGLLMLGPSKSFYVKEINEEEVRKLFYVRIELEKLALRESID
                     SVDMTAISNLIIRMRKHLSNREETMENVPYDIDYKLHRLILDNCGNSYLIDIMQKISI
                     LITRMRNVIKYYLPQQQKDWIICEMEEHLEIAEGILEGNLKKAEMALDSHLKHSMDII
                     CSILGSSKVDYKTATSFLELEAKV"
     gene            21242..21541
                     /locus_tag="PLD15_00125"
     CDS             21242..21541
                     /locus_tag="PLD15_00125"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPB62328.1"
                     /translation="MKQAILLSQNDNVATLLERAEPGETIEIKGTGPAGKIVSRNSIP
                     SGHKVAVKDMMPGDTVIKYNNRIGIATDEIYAGQHVHVHNVKSARTESGKGGEQA"
     gene            21553..22731
                     /locus_tag="PLD15_00130"
     CDS             21553..22731
                     /locus_tag="PLD15_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HPB62329.1"
                     /translation="MQKTIKGYIRKDGSVGFRNYVLILPSVICSSKVAERIASSVPGC
                     AVARHNQGCAQLGEDFHQTRRTLINTALNPNVAAVIVIGLGCERVSPHELRDLIAQAG
                     KPAELIMVQEVGNGEAIRLGREKARDFVAEVSKIERQSVPLSALTIGVECGGSDFSSG
                     LSANPVVGQVADLVWKNGGRVILSETTELVGAEHLLFERMSDDSQKERFTRMLERMIN
                     ESRNNSRDMVDRENIPNNISPGNVRGGLTTLEEKSLGAMIKGGKVPIVGVKEYGEIIE
                     ATPGLYLMDSPGYDVESVTGMVAGGASIILFTTGQGTPTGNPIAPVIKVTGNRDTAMK
                     MKDSIDFDCSAVISGEETLEKSGERLFDMVIDVSNGAETRSELLGQDDYSIWNVGIKL
                     "
     gene            22707..23831
                     /locus_tag="PLD15_00135"
     CDS             22707..23831
                     /locus_tag="PLD15_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPB62330.1"
                     /translation="MERRYKAMIPGKDELERIFLEMCKYDTTNPPGNEESLALFIKET
                     LGSYGVNCTIQRVGDGRVNLLAKIDRNGGLPYLLFAGHMDVVPAASGWTGDPFKPVVE
                     NGRVYARGSADMKAGLSSMMAAFIDLSCDERFGGNLAFLATCDEEVGCSGVKRFLEEN
                     TLEITGALIGEPTSMRLASGEKGAVWSELNFKGKSAHGSQPQEGINAVLLLIDAYQRI
                     AGELGGIEGLTVSLNKVSGGSKENTVPDSASCVLDIRFMSNISPPEVTGVIDKVLSRF
                     EGAGQKVLLTRNPFSSSGRLSDCVRSTLVEIGREPEPLTMTYFTDGAFIAPSGIETVI
                     LGPGAASMAHKANEYVELEEIHIAREIYGKIVRNFFQGCV"
     gene            23833..24918
                     /locus_tag="PLD15_00140"
     CDS             23833..24918
                     /locus_tag="PLD15_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPB62331.1"
                     /translation="MDMTVIDSPSLRNLCVDILVAEGFGQDVSADIVDVLLEADLRGI
                     PSHGVARLRRYIDERNAGHIKLNETPSVKYETPLSAVVDGRGGPGQSVSKWAMNLAIE
                     KAGNNLAGFVSVRNSNHYGITAYYSEMALKRDMIGIAMTNSYPLVVPTFGREAVLGTN
                     PFSIAIPGSRHPFILDMATSVVTRGKLEVYDRLAKVIPGGWAVDSLGADTNSPGEVLA
                     NFNNRRPGGILPLGGSGEEFGGHKGFGLSLLVDLVSAGLSLGSWSALTYSEGVAGVCH
                     FFGAINLRLFGEPESLKRQVQSIIDGVVSSGKACGSEKIYYHGEKEELSRISSLSKGV
                     TLDPKTVRNLVSLAEKNNLPIPDQLLQ"
     gene            complement(24921..25193)
                     /locus_tag="PLD15_00145"
     CDS             complement(24921..25193)
                     /locus_tag="PLD15_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62332.1"
                     /translation="MSLAVTGYDKFLHYSVSYTAYGLSSFLLGDVGGFIFTSALGIGK
                     EVWDFTSRRGTAEIEDLIADFAGIIAAYHHIRSLDFRPFFIFQVVF"
     gene            complement(25259..25822)
                     /locus_tag="PLD15_00150"
     CDS             complement(25259..25822)
                     /locus_tag="PLD15_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62333.1"
                     /translation="MKKVALVALLLSAVIMGFSAPKALIGGAFGINATNPIYQPERWG
                     AGALDLSLQLPFTEKIGTTLSAQAAVRYPTNKIGLLNADIYFQIFEIFNLKLRLLVSV
                     ASVMDSNFGEPGWMIGPLDLGAPHLAIGGGFQLMAPVTEKVTLNGYARGYRVYKYATR
                     QEGNFPGGDYWPLPEFFSVGLGVLYSF"
     gene            complement(25966..26409)
                     /gene="smpB"
                     /locus_tag="PLD15_00155"
     CDS             complement(25966..26409)
                     /gene="smpB"
                     /locus_tag="PLD15_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPB62334.1"
                     /translation="MKIVVTNKKARFQYHLLDSFEAGMELMGTEVKSLRLGGASLVDA
                     YCKIENGELFLIDCNISQYSHGNLWNHDPRRKRRLLMHRKEILRLDQKTKEKGLTIIP
                     TKIYFNDSGRAKVEIAMARGKRLFDKREDVARRDVERKLRQQVDI"
     gene            complement(26590..27699)
                     /locus_tag="PLD15_00160"
     CDS             complement(26590..27699)
                     /locus_tag="PLD15_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HPB62335.1"
                     /translation="MLLELFKERVEKVRSLFEENGADALLISKCNNFSWFTFGARSHI
                     TLNATEGEASILVTRDGVYLLINNIELQRLKDLELPEEIQRELEVVEYDWFERTSAEK
                     RAIDSIVKGKVISDTGRYDTGTVDLVSLRSVLSDYEVGFYSDLGRECDEIFSDVVPSF
                     VPEMTELEVQGIMYREMAKRDIEPVLTLAFSEESTLTYRHNLSRNTRLGRRGFVSICA
                     RRRGLIISSSRSFMFEEVEGIREQHRCNCYVDSVAIVSSRPGKKLSDVFEKIKEAYAS
                     QGAPGEWRFHHQGGLAGYSPREVVADPYTSIVLKSGNSLAWNPTIRGTKSEDTVLLLD
                     GQNRIASYPRSSTWPALTFEIDGQTVRRPDIVLLK"
     gene            complement(27737..28054)
                     /locus_tag="PLD15_00165"
     CDS             complement(27737..28054)
                     /locus_tag="PLD15_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPB62336.1"
                     /translation="MIVTVVDIYVKSERIDEFIEATTLNHKSSIMEEGNLRFDFLQDR
                     SKPNHFLLYEAYESEEAAAAHKKTDHYLKWKNTVESWMEKPRTGIQTNVIEPSGIASW
                     KHR"
     gene            complement(28180..29349)
                     /locus_tag="PLD15_00170"
     CDS             complement(28180..29349)
                     /locus_tag="PLD15_00170"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HPB62337.1"
                     /translation="MKHGQSHSTEVGSHRVILYNAPSEIERALGSFCFNGYSQEKKLL
                     FIYDRLTLVDLQRAIEPYGMNLEELRRSGWIEVASVRDTYSRDGVLDLERMAKELEEK
                     TREALDSGFEGLCVAGEGSWIFDESFDAAKMVEYESKMDEFISENPLDACCIYDTSIV
                     NKRMLLQISSLHPFAFDTDSFVPNYDYFKDAYRNNCESDECRQLYRGTATMALEMFRI
                     RGFYAEDHCENVSRLASAIARKMNDSKLAGELETAGKIHDIGMMSLFSDMIGKPGKLT
                     WFESQLLHEHPLVGYELVKKVPFPRTIARAILEHHERLDGSGYPLGLKGDEISIGGRI
                     LAVAEVVSAMSFYRTYRDDYGIEVALNEIDDKAGSFYDSDVVKTCFKCFEEGFKF"
     gene            complement(29685..>30694)
                     /locus_tag="PLD15_00175"
     CDS             complement(29685..>30694)
                     /locus_tag="PLD15_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB62338.1"
                     /translation="IPVQFLSTSGESILQLGGRLLSVIPLKEGFANGMVVDYSDRESE
                     YRLLTRSLTSLAGQSVVFMRVGKPVSELSNIMRNLLWALLSLVLLTAAISWIIGLALA
                     GYVLNPFKESYQRLQRFTADASHELKTPLSIIRLGLDMLGEKELDPEVSSKIGMIDTA
                     TRNMQNLVNQLIKMARIQSSQEIKAKAEPVNIKRLLEDIALSYRPLSDPRGIEIEIEC
                     EDNLVSTVMIEPLTTSLGNLLENAVKFSYDGSRVVLKSFLRDGYLYIQVIDNGPGIEL
                     VEHEKIFERFYKSERSHNSTGSGMGLAIARELVGTMKGEINLVSEPDKGSTFEIKLRA
                     G"
BASE COUNT         7646 a         7434 c         7921 g         7693 t
ORIGIN      
        1 gataataaag cgcttccgcc gaaatctccg gcgagcggga gagtctatct actatcgtcc
       61 tcttatacca tctgtaaaat gcaccgtttt cctttccggt tccagtcctc acagctcccg
      121 gatgcattgc gttcatcgtg actccgcttc cctcgaagta ttcattgaag atgatcatac
      181 cgaggagctg tgcggttttc gcggatccgt aggctttaag accgctgtaa cgcctctttt
      241 gccagttgag atcgtccaac cttattcccc acaccgcaaa acggtgacct tccgaattca
      301 ccatcaatac acgcgccttc tcctgcgact taagcttatc tttaataaga tagtttatta
      361 cgaaggaaga gagatggttg acgacaaatg ttgtttcaaa gccatccccg gttaattccc
      421 tgcgcttgag gaagactccc gcgttgtgta tcaggacatc tattggctct ttcatcgcag
      481 agagttcttc tccgacgctc aagctatctt tcaggctgct gaaatctgct atcatgtaag
      541 aacactcaac accgaagtct ctcttgagtt cgtcgcacag cctttccgat ttctccccat
      601 ttctgtttat cgatagaata cgcgctccat ggcttgcgta cttcctgcat gtgtagtatc
      661 cgattccaga tgtcccccct gtgattacca tgagtcggtc cttgaaatca tctccgcaaa
      721 tctctggatt cgccccgttg ttcttaatca tcgcgaatat gttcgaccat ttgtattgtt
      781 tccagtatct ggcaattgcg ctcttctgct tctccattgg caacacctat ccgaaatgtt
      841 ttctcatgaa ctttctgtaa ggctttctga atgccgggat gttgtcgaag atatcgcccc
      901 acaggtcgta gtagtctctc tgctcgacga ttttgcccct ttcattcaaa gaaaggcggc
      961 tcgcaccgta tatcaccgaa gtcctagtct ttttgaagag aattgtcatc tcccactcca
     1021 cgaagaccgt tctatcttcc attacgactc tcaagatggc catcttcagc tctttcgatc
     1081 tctctgcaag cctttctgtc atcttcttga attcttcaag ccctctgatc tcttgaattg
     1141 tgtctctaaa tagaatgttc tcatcgtagt agggcaaaat atgcgaccaa tcgggttttc
     1201 cttctgtgtt gtaagttttt gaccataact cgcgtatttg atctgcactt tctataacga
     1261 attcatccat aattacctcg ttaactaaaa acttctgcct ttatatacgg gcaaaaagcc
     1321 ggtttgattc tcttgcttat tcatggagtg ttatactgcc agaacatgct tgattctgtc
     1381 atcacccgag tccctattcc tttgtttcag taacatacag gtagagcgtt tcaacagatt
     1441 gggaatatac catgacttct tcccggctca agggagccgg acgggtctct ccggcggccc
     1501 atagttccac ctgatcgaag aaatgaggac ttcgcggatc ctcggacact ccgggcggta
     1561 ggaatgcgcg ggagttgtct atatccgaga gatcaaccac ttgagtatat gaatttccta
     1621 actgcgacca gatggtgttt gccgatgtag cagtgagata gggcatatat cgattcgcgt
     1681 ccgggtatat cgatggaaag ccttcaagat tagcctgata gatcatcact tgcggccttt
     1741 tctgagagac gctttcccgg atgccggcta cggcagtttc gagaacctga ttgacccaga
     1801 ttgtcacctc ttcctcgtct ggcaacgtcc cagatctttc aaactccgcc aggacagaac
     1861 gaaggaggta aatattgccg gcgtggcctc caccgtattt cgaagcaaga agagcggtag
     1921 ctgtattact cctgaggctg cggtgcataa cctggacgag agtctcggca agatcagaga
     1981 ccctgctgtt ttctatcagg ctataatccc agccatcgaa ggcgcttaga aacctcgaag
     2041 aattctgtga aagcgagttc ctctcaaaca aaatggccgc gagaatcaga aactcacggg
     2101 ctattgccga aacattatcc cggtgaatct ctgaaaattc tgtaaaagtg aaaccatctt
     2161 gatcattaag cagctcgtaa agcctcatac ttctggggtt atggccggtt cctccggtac
     2221 caatcgtgag agaatatgga taccaggcgc ccacaggcag gtggtttgca ctggcaacga
     2281 atcctgtaga aggatttaga acatgcggaa gctcttcaaa cggtacgtaa ccattccatt
     2341 cctcgtcacc cgtccagccg atctggggaa gcccgggcct tccagaacgg atcggaattg
     2401 ccgcggctgt atagtatccg atgttgcccg atacatccgc gtacacaaca tggagaccgg
     2461 ggaacatgta attgcccaat ccgcaggtga actcttccca gtttctcgat cgcatcattg
     2521 ataacattcc cgttataggt gtcttcagct cttgggtggc gacgtatttc aacgagaagt
     2581 attccgcagg tctcgcccca ttgagaaacg catcgacaag gggtccatga atcgttttgt
     2641 tgacagtgat ttcaacaggt tcttcatcgc gaatatcgat cacttcggta taagactgaa
     2701 ccggatacca gttctctttg aagaggaagc tggatccatc atcatcgagc ctctcttgaa
     2761 aaaggtcgct gttgtcggaa ccgagtgcag ttgctcccca cgcgagatat tcgttccagc
     2821 ctataaggaa ccccggtgaa cctgcgacgc aaattcctct tacgttgtag ttgccgccgc
     2881 taagatgtat ttcataccag agagaaggaa gcgtgaccgc aatttgaggg tcgctctcaa
     2941 gaagaggact tcctgtaaga ctcttgcttc ctgagatcgt ccaggcatga ctggccttca
     3001 atgaatccca ctctagccag gcactcaact catccagttt ttcgttttcc attagacggc
     3061 ggtatgtatc gggatcggtg ctaataaaat cggcttcagt tactattgcg gcgaaatcat
     3121 ctataacaac gggatcttga gtcactccgc tccgcaaccg ttgctcatag ctgcggagag
     3181 ctgtcacttc gttttgccat gacctgtcaa acctctcgga gatgcgcatc cacactgcga
     3241 tgctatcggc aactgtccat ggttctggag tgattccggc ttcatagaaa gcaggattca
     3301 gatcgtcgag atgagtctcc agatagtagt tcactccacc ggcgtaagct tcgagtatag
     3361 tctttgtctc accctgtaat tggtcggcaa ttttctgtgc gtgtctgtaa agtccgagtg
     3421 tacgcataag tttatccgac tccaaccagt ccttaccgta gatttcggcc agtcttcccg
     3481 caacttttcg ccgggaaagc tccatttgaa acatgcgatc ctgggcagtg gcataacccg
     3541 ctccgaaaaa gagttccgaa tccgtttccg cgtagacatg ggcgactccc cagtaatcac
     3601 gaactatctc gatctgaccg ccaaacaatc tggctggaga agagacgaaa gatatcaaaa
     3661 cgaagatcgc cagtaaccag aacactgttt gctttctcat gtctgccccc ctggactcat
     3721 catctggagt taccttacaa ttatagagcg ctttgctctg aacctgatgt tcttcacgga
     3781 gacagttctt ttttaagtta aagttcatat gttgagaatc tcataccaac catggttcga
     3841 ttacgcgaga tttcccgcga atctttttca aatgtgtttg cccgggctct gatagaataa
     3901 tactgtggtc attctttttc tcatagagag attggagggt tacggatgag tcagctggta
     3961 gttactggga acacgagact tactggagag ataagggcaa gcggttccaa gaattcggtt
     4021 cttccaatcc ttgccgctac ggtgatgata gaagaacccg ttgtgataaa aaacgttccc
     4081 gagctcaaag acgtggagac catgatctcc atacttcagc agataggaaa gatcgttact
     4141 ttctataacg agacggtaac cgtgaaacct ggcgaggtgc tcataggatc ggtgccgtac
     4201 gaactggtaa gtaagatgag ggcttccttc aacgttatag gcccgctggc catgatctgt
     4261 ggctgggcca gagtgggcaa acccggaggt tgtaacatag gtcaaaggcc ggttgatttt
     4321 cacctcaaag gactggaggc ttttggcttc aagatcacgg aggaacacgg cgatgttctg
     4381 gccatgatac cttcggagtt caagaaagag ataatttata cgcttccctt cccgagtgtc
     4441 ggagccaccg agcagttgat gacaacggcc gccctgatga acgggaccca tgtgataatc
     4501 gagaatgccg cccgcgaacc ggagataacc gatctccagg gctttctaaa ctcctgcggc
     4561 gcgagagtca gcggcgcttc aaccccaact atagaggtgt acggagtttc cagtcttcac
     4621 gggggcgatt acagcgtgat tcccgacagg atcgaaacag ggacctatct ccttggcgca
     4681 ttgactacaa ggggagatgt caccataacg ggtgccaggt acgatcacat aatctctctg
     4741 ataagcgtac tggaagagat gggggccggg gtggagaagg ggaacgattt tatcaggggc
     4801 ttctggaaag gaccgttaaa accggtcacc gtttcttcag agccttaccc cggttttcca
     4861 acggatctcc agccgctcct cacgagcgtt ctcactaccg tggaagggac ctctattctc
     4921 gaagagaacg tgtttgaaaa ccgtttcggt tatgttgacg agctcaacag aatgggggca
     4981 aggataaaag tatctagcag gcaggccttc ataatcggtg tacacagatt aagcggcgca
     5041 gaagtggtgg ccacggacat aagggccggc gcggctctgg tcatggcagg tctggcagcc
     5101 gacggcgaga cgaccataaa caacgtgact cacatattca ggggctacga gcggttccag
     5161 gataagctga gcaggctagg agcaaaaatc gcttattatc ccgacgatcg gtgatcttca
     5221 atcgcggccg ggtgcaataa ccggaagaga attgagaagt cccgatgccg cgtatcaatc
     5281 actggtgaag gtgttttgct gttttgtata tcaggatggt gttcgattga acgggcaacg
     5341 attagacaaa gtcaggaatg cgatttcgat tgtgggagag cgcatgcttt cgtgcgatct
     5401 ctgcccgaga aactgtaggg taaacagata cgagacggcc ggcttttgca ggcagtttgc
     5461 cgatcccagg gtttcggcga tagttttgca cttcggcgag gagccgcctc tggtaaagga
     5521 tggaggggcg ggagctgttt tcttcagcgg ctgcaccatg agctgcatat actgtcagaa
     5581 cttcgccttc agccagaaaa acaacggcag agagatttcc gccgaacggc tcggagaggc
     5641 tttcttgaac gtagaaaggg aaggcgccgt ctgtctggat ctggtaactc ccacgccgaa
     5701 cttctacgga tttctcaggg cttacgaata cgccctcgta agaggttttt cacttccgat
     5761 tgtcttcaac accagtggtt atgaaaaacc cgataccgtg aaactgctgg aaggtgttgt
     5821 cgacatttat cttgccgata taagatacac cgacgaccaa atagggaaga aatactccgg
     5881 cgttgccgac tactggtcgg tcaccaggaa agcgatcaaa gagatgtttt cccaggtcgg
     5941 ctctttcaaa gatgaggaaa tgagaggcgt gataatcagg catctcgttc ttcctgaaaa
     6001 tctggcaggg acccaggaga tggccgaata cgtcgccttc gaactttcgg cctccgttcc
     6061 catatcgcta atgtcgcagt acagacccgt ttacagggcg cgtgagtacc ccgagatatc
     6121 cagaaggatc acgcaggagg aatacaacga cgcgctcgag cttttagaca attacggttt
     6181 gacaggatgg atgcagcact tcgatctgga agaaccgttc cgtgccaggc cggtcgggtg
     6241 ggatcaatga tattctcgat ttctctgttt ctctggataa ccttttttgg aaagatcacg
     6301 ccggcggcct tgatctcggg ttccatcgtt tcaggcttcg tccagtacat ttcctcgaag
     6361 cttgttccga agggcccgtc tgtcagaacg gctttcaaga ttctcctgtc gttaccccca
     6421 gccattttcc agtctttcag gcttcttttc tccaaacctg tcttcacggt cagggccgaa
     6481 gagatacctg aaaaccgaat cgaggagttc ggcaagatca tttcggtgac gatgactccg
     6541 gaagaaatag tcgtttccaa agatagagaa ggttttctga tccacgaggt gaaacgttga
     6601 tcttctacat aacgatgttc tcccttatgg cttccatagc catgctggta tggttttcag
     6661 ccggccagga cccgtggaga ttgctcatag gatattcgtc catctccacc aggcttctcg
     6721 ttgggatagt ctttatagaa ctggtgacac aggtcgattt catatccgga gttgccctgc
     6781 tttttctaat actaaatacc agcggtacga taatcgcggc gtattatctg ggggtgcgta
     6841 gataatggaa atcgcgggtt gcgtttttct ctttgtcggc cttgttttcg tcgttgtagg
     6901 gaccctgcgc tcggtaatcg cccacacaat agtcgccaca ttgcactttt taacgattgc
     6961 cgacaccgtc ggcctgattt tcataatcgt ctcggccttt ttcttcggct gtctctcgat
     7021 aatcgagacg atagcttttg tggctgcact gatggtaacc ggtccgctgg tcactcacgt
     7081 tatcgcccgc gcttatatca actcggaggg agagagatga ataccgtagt gatcatgatc
     7141 ggagccctct atctgttgag tactgtaata gtcgtagctt ctaagagcag cttccaggcg
     7201 gttatctggt tcggaataat gggttcgctc tcggcgatcg tcatgcttct ggctggagcc
     7261 ccggatgtgg cgatgacaca gttcacggtg ggcgttgctc ttgttctgat agtttatatt
     7321 atggccttga agcgtcagag gagggtgcgg ctgggtttcg tgaacgttcc ctcgatgatc
     7381 gagatcggtt cgggcggtct gaaggggctg gaatgggaga tcatgaagag aatagacgaa
     7441 aaggaaggtt accatataga ggcggcggcc tttgaaagca gagaaactgc tatcaaggcg
     7501 gtcgaagaac atgaaataga tatcctgtgc ggagccttca ctgaggaaga actttccggt
     7561 agcacccggg gtatcgctta tcttgaaact tcgatcttcg attttgaagg agtcgaagtg
     7621 gatttcgtga agctgaagca attgagcaga agctcggttt caccacggcc cgtctttttg
     7681 aagaagagca attacgtttt tgtcgtctca aagagttccg gtgatctcgc cgaagctctt
     7741 tccagcgata tagagaatat gactaaggaa ggaaagatgc aggagatagt ggggcgatac
     7801 ctgtgaaggc catgaagatt ctgggtgtga tcgctgcggc ggtgattttc ctgtttgtgg
     7861 tccttcaggc aagaatcgat ctggtttttc ccgaagggct ggaagagata atcgagcgta
     7921 cgaacattcc aaatgccgtt acagccattt atctggagac acgtctgtac gatactattt
     7981 tcgaggtcat cgtcttcagt atcacggcac tcggggtgac gacgctcttc agctcgcttc
     8041 caaggagcgc tgaaggaagt cagcaggtct tcggctcggt taccgtttac agcggaggtc
     8101 tggccgcgct ttcagtaacg ctctttctgt atgtggtgct ggaaggacat atcagcccgg
     8161 gtggaggctt cgtgggggga gtcgttctcg ccaccgggat agttacctat ggattgacat
     8221 cgaactttgc gaaggccaac tcccattacg ataggttcaa gatcaaaata atggaaaatg
     8281 ccagcctttt gataatcttc tcgcttgtct ctttcatcgt tttctttccg gaaacgcacg
     8341 ccaggttgct tgcctcagga gagtttggag aggtgcttag cggcggattg atcccggttc
     8401 tgaatattct gatcggcata aaagtctatg ccggagcgtg gaagatgtcg agcgaattta
     8461 taaacaggcg gggtacttta tgacgggaaa cattatcttt tacatttccg tggccggcat
     8521 gctggttgga acggttggta tgatcctttc ccggaacatc attaaaaaga tactctctct
     8581 gggaatcgtg gagacatcta taaatacact ctacaccggc atcagttcga gaaagagcat
     8641 tataccgccg atcgtgaacg gagatttttc catcgcttac gccgacccgg taccgcaggc
     8701 cgtcataata accggtatcg taatatcctt cgcccttctc tgcctgtcgc tggtcttcgc
     8761 gataattatc tacaacaaat atcacaccat ggatatcggg accattgaat cgaacttcga
     8821 gagggagaaa gaatgatctt cacgatcttt ctgcccgttc tgggtggaat aatctgtctc
     8881 atattgagag agaggagacg actctgcgcc ggtgtggcgc tatttgcaac gataatcaac
     8941 ggaagctttt tgcctttcaa tctcttcgaa gagttcggta tgaacctcgg gaactgggga
     9001 gaacttggca taagactgac catggatgat ctcgccatac ctttcatgct ttccactttt
     9061 ctggtgatgc tggcgattac tcttaactct ctgaggaaga attatgatgg cttcttctat
     9121 agcctgatcc tcatcatgta tggcacattg aattcgatct ttctttcgag agatcttttc
     9181 aacgtcttcg ttaccgtaga gctggtatcg atcatctcct tcatactgat ctcctacgag
     9241 aagaaaggcc gccaggcatg ggccagtctg aagtaccttc tcctctcatc gctcgggttg
     9301 aatttctttc tgctggggat aggactggtg tatatggaaa ccggctcgtt cgccatggaa
     9361 gggctgaacc acgtttcaag tatcgcctcg gttttgatat tcggaggact ggcggtgaag
     9421 tccggcctct ttctcttttc catgtggctt cccgacgccc actccaacgc gccggtagaa
     9481 gtttcgccaa tcctctcggg tctggtcgtg aagatcgatg tctatctctc cataaggttc
     9541 gtccagtacg agtctttcgg gtggctgaaa gacgagttcg tgtatatagg tatattcagc
     9601 gcgattgccg gtattatctt agccgtgaac tccaaagatg ccaaacgtct tctagcctat
     9661 tccaccattt cgcaggtggg gtttatgatg gttacctgtg acaaggcctc ttcgtggcac
     9721 ggcttcagtc atgctgtctt taaaacactt ctcttcctcg ttgtcggaaa tgtatccacg
     9781 aggctcggaa ctaaggaata cagagattgg tcgggaagga taacccgcat agagtacctt
     9841 tttcttctgg ttggctcgct ggcaatttcc ggcttcccga tgacatccgg ttgcgttacc
     9901 aaagaagtga taatgcacga cgcctgttgt ccctggttga aggttctcat gatgctttcg
     9961 tccgtcggta cggccatgat cttttctaaa ttcatctttt tgaagccctc caaagagacg
    10021 ggcagcctcc ccaccggtac gctcgcagcc tattcgattc tctcggtcgc cataatagtt
    10081 cacggaatcg taggcttcga gatctacatg ttcgaatcac ttctgatgat cgcggccggt
    10141 gttggcttgt atttcctgta caggaaattc atgaggcctc tgcctgtctt ttttgaaagg
    10201 atcgacaact ctctggcgat ctatctcatc atgtttctct tttcaattgg attcgtaatt
    10261 ttttccactt gaaaagggcg gtcaattcgt ctataatcgt ttttgtaggc gagtagctca
    10321 attggcagag cgacggtctc caaaatcgta ggtcgggggt tcgaggccct cctcgcctgc
    10381 cagaaaaaga gaggattttt cctctctttt tcatttttca tcgagtctta cctattgctt
    10441 ctccacttgt gtggaaattg catcgttttc ataagaaaaa ctcagttact aacctttaat
    10501 cgttcgagat catccaggat tttgccagat cggttctcaa aaggttgagc gatttataga
    10561 aatcatggta cactttgcat gatgtataat gcatcatctt aatgaattcg caattttaga
    10621 ttctttccgt gagaacgggg tacaacctta ccccaaatat ggaggtgttt gaatgaaact
    10681 gaacagggta cttgtgctgg caattgtctt gcttctggga gtcgcagctc tgggtgcaac
    10741 tataaaagtt gctcttgtag ctcctttcac aggtttgggt tcgattctgg gcgattacat
    10801 caagatgggt gcgcagctgg ctctcgaaga aatcaacgcc gccggcggca tcaacggcga
    10861 tatgctcgaa atgattgtct atgatgatgc cgccaatccc agtaccgccg caaacgttgt
    10921 tagaagagcg ctcttccagg ataatgtagt tgcggtattt ggaccgaaca tgagcagtgc
    10981 agtcattgga gtccacccgc tggcccagca ggcgaaaatc cccatgcttg tcggagctac
    11041 ttctccatcc ttcaggtaca caaagattcc caacgatttc cttttccgct tgagagctga
    11101 cgacgaagtg aaggttgtcc agcttgtcaa atacgccgtt gaggttcttg gtatcaagaa
    11161 gcccggtgtg atttacggct cgacggatta ttgccttgcg gctctagaag tagcgaaggt
    11221 ggagttcccc aagtacggag tggaaattgt agcgatggaa cagatcaaag aaggcgacaa
    11281 ggatgccaca ggtcagcttc ttaagctgaa aaacgccggt ttcgacggtc ttatcggtct
    11341 gactcacgag tcggaagccg ccgtaacggt caatcaatta aggcagcttc agatcgatgt
    11401 gcccattata ggtttctcgg cgtggggagt tccggccttc acagatctag ctcccgaagc
    11461 ggcgatcggt gtttactcgg tccagggttt caatcccgtc gatcccgatc cagcagtcgt
    11521 cgcttttgtt gatgcttaca aggcaagatg ggacggtaag gagccgagcg atcccgcaca
    11581 ggcgtactac gacggaatgt atcttctggc agaagcgatc aagaacgttg gaaccgatgg
    11641 cacgaagata gcccagtacc tggcgacaaa ggcaaatata gtcggtgtcc agggagaact
    11701 taagtgcgat gagcaccaca acttcaccaa cgtttgtctg atctctcagt tcgacggaaa
    11761 agactggaag ataatcaaca agatgttcta agcctcttgg cgagagggag gcaaagggtc
    11821 ttcctctcgc ttttatttat ccagacaagg agtgtgaatg atgcttcaag acattctcca
    11881 gaatatagtt tcaggggctt caataggcgc tacatacggg cttgtcgggc tgggcgttgt
    11941 tttcctctgg ctgacgatct ccaggatcaa cttcgccaat atctcatcgg ccatgctggg
    12001 agcctacctt ttctatactt tctatacgat ggtcaatctg gggttctttc tctctttcgt
    12061 tctgtccgta ggcgtaatcg cgctttacgg gatcggcctc cgggccttca tatatgaacc
    12121 tataaggaag aggggaggag gaagactgga atttgtagtt gcaacgttga tgctttgcac
    12181 tttctggctc aatttgataa ttgtgaccta cggggctctt ccgaaaccct tcccacctgt
    12241 gttcggcggc aacaaagact tcatcacgat cggtagcgtc aggattccga cactttacct
    12301 gaatatttat ctggtgatcg ctgtactgat ggtactgatc tactttattc tgaacaaaac
    12361 tcttatagga aaatcattcc gcgccgcggc tcagaacaga gaggccgcag ctcttatggg
    12421 aatagatgtt aaagttacca cttcactctc attcatcatg gcgacatgtg ttgtgggaat
    12481 cgcaggtata cttctcgctc ccatctactt cgtatctctt gaactgggcg gaggatcgat
    12541 cggggtaaag ggcttcgctt cggccgttct cggcggtctt accaatcctt acggaacgat
    12601 actcggaggg atcagcctgg gacttctcga gaatttctcg accctctata tatcttcgac
    12661 ttacagagat atcatatcct tctctgttct cgttgccgtg ctcatactga agccttccgg
    12721 aatcttcaac tggaagagca agaagatata ggaggcgtga gatgaagaag ctgttattta
    12781 tcgcgggagc gatccttgta ataatactct ttccgatcct caacaagaat atgtaccacc
    12841 tgtatctgat gaacagggct ctgattcacg ccatcctcgc gacgggcctc gtctttctca
    12901 ccggatttgc gggtcagatc tctctcggac aggccggatt ctacgcgatc ggcgcctaca
    12961 gttcggcata tttcacggta aaactgggta tgcccatacc tgtcgggatc atcataggaa
    13021 ttgccataag tgttctggca ggttttttgt tgagcattcc ctcgtttaag ttgaaggcct
    13081 ttttcctctc gctcgttacg atcgccttcg gacagattgt gtggatgctg gttataaacc
    13141 ttacgccaat cacgggcggt ccttacggtt tctttggaat tcccttctat tcggttggca
    13201 ataatatgct ctccttcggt caggtcttct ggctttttgg agctctgctc attctctgtg
    13261 tttatataat gtttagaata aagcattccc acttcggtag agcgatgctt gcgatgaacg
    13321 acgatgacgt agcgacggag agctgcggca tttcgacgaa gagactgaaa attgcggctt
    13381 tcggtttcgc cgcaggcctt gcaggtcttg caggggccct ctacgcacat ctggccggat
    13441 ttctctctcc cgagccgttc acgtttttcg aatcttccaa cttcgtggcc atggccgttg
    13501 tcggaggttt gaggcacatg agcggcggag tcgtgggcgg tataggctta acgcttctcc
    13561 cggagtttct caggttcggt ggctgggaaa actattatct gatggtcact tcgctgattg
    13621 tcatagtggt aatcatcttc atgcctatgg ggctgggacc gataattgaa aacctgttca
    13681 ggaagatctt caggctgaag agaagcaaca caaagacgtt gtaaggtggg gtggaaatgg
    13741 acatacttag aacggaaaac atgaccaaga gcttcggagg ggtagtcgcg aacaacaacc
    13801 tctccatagg aatccccgaa ggcaagatta cggccctgat cggtcctaac ggttccggta
    13861 aaacgacatt catcaatgtc gtcacgggcg tacaccccat caccagtggg aaggtctttt
    13921 tcaaggaccg ggatatctcc ggtctggatg tcaatgttat aagcagaatg ggtatcgcca
    13981 gaactttcca gaagattcgt ctgtttgaga atctatcggt tatcgaaaat gttctcgtcg
    14041 cgaggaagcc gttctacaaa tccggtccca tcagcgtggt tctcaatacc ccgaagttca
    14101 agagggaaga gaaggagaac aaagagaagg ccttagagct tttgaaactt gtgggactgg
    14161 aagacagggc ctacgattct ccaacgggta tggcgtacgg gttgagacgg tgtctcgaga
    14221 tagccagggc actggcgctg gaaccacagc ttctcttcct cgacgaaccg gccgccggaa
    14281 tgaccaggga cgaattcaaa gtgataatgg atctgatgat aatactcaag gaacgcggaa
    14341 tcacgactct tctggtggaa cacactatgg acttcataaa ggccgtggcc gactgggtat
    14401 atgtgttgaa tttcggtcag gttatcgcgc agggaaagtt cgaagagatc gaaagggacc
    14461 cgctggtcct gaaagcctat ctgggggagg aatgaacatg tttttactgg aaaccagaga
    14521 cctatcggtc tcttataacg aagttcccgc tctcagaaaa gtctctataa aggccgcgaa
    14581 gggcgaagtt gtctctgtgc tgggtccaaa cggagcgggg aagacaactc tgctgaagag
    14641 catatcggga ctcgtgcctt gcgagccagg aagcaaggtt ttcctcaacg gagaagagat
    14701 ccagaatctt gctccgcaca agatcacaag actgggtgta attcacgttc ccgaagggag
    14761 gcaggttttc gccgagctga cggttcagga aaacctcgaa gtcgccgcga cgagggtagg
    14821 cctggcaaag gccagaccca acatcgaaag ggtctatgaa ctcttctccg agctgaagcc
    14881 gagaaagggg cagatggcag gaactctttc cggcggggag cagcagatgc tcgcaatagg
    14941 cagggctatc gtttccgagc cgttgataat gatgatcgac gagccgtcga tgggtctggc
    15001 gccggttatt gtgaagagga tattcgaaac cttgaagaaa ttcgtaaaag acaccggtct
    15061 gactatgctc atcgtggaac agaacgcaaa actttccctg ccaatgagcg acagagtata
    15121 tatagtctct cagggtcaga taaaacttga aggaagcgtc gaagaagtga aaagcgacga
    15181 gaaaataaga gaaatgtact tcggtggcaa atgaaatgct tgtagaactt gattatacga
    15241 caggagagaa actcgggctg gagattacgg ctggcagtct tctcgcaccg ttgaaaatca
    15301 cgactgggaa aggggctcat agagatctcg aaagctcgct ttcgacggcc gataacgtcg
    15361 atctgtacac tatggcgcgc gaagccggca ggatcgtctt catagtggag gatcatacga
    15421 gacactcgcc ggttctcggg acgctcgggc atctaaaatt cgagtttgga aggcgggcga
    15481 ttccgtggga cagggtgaag cttctagtcg ccaccggtac ccacaggaag atgactaaaa
    15541 tcgaacttgt cgagaaattc gccagcttcc tggccagtac cgaaataata cagcacgacg
    15601 cggctgacgg cgtcgaaatg aaggattttg gaaactacct ggatgttccg ttgaagatca
    15661 atagaaacgt ggagagcgat ctgctggtcg cgattggttc gatagtgccc caccgctttt
    15721 cggggtggtc gggcggcgca aaaatggtag tgccgggagt agcttcatac gagacggtct
    15781 tccagtctca cagaatggca attctgaaca gctcggccga tgtcggcgtc gtcgaaaacg
    15841 agtttcgaga tctcatagac gagacgggct ccagagtcgg ccttgatttc atcatcaatt
    15901 attattacgg tatagacggc tccgttatgg gctgcgtttc gggcgaccat ataacggcac
    15961 acagaagggg tgtcgaactg gccagaaacg aattgcttcg tgtcttcgag cgccggtcgg
    16021 acatcacggt gatatcatcc tatccatcga tcacggattt ctggcaggga ggaaaggctc
    16081 tctacactgc cgaccttata accaaggacg gcggagatat tgtaatggtg tcgcccctaa
    16141 acgaggggtt cggcgatcat ccggcatttg cgaaactcct aaggctaaag agcgccgaga
    16201 ttctcgagga actggacgga atgaccgctc aggatccgct ggcctacgtc gccgcctacg
    16261 ccgtcaagaa tataatggag agaaagcgtg tccacatcgt gagccatacc gcttaccggt
    16321 ctatgttcaa cgaaatcggc ctgtcggtgc acgatgacct gcaaaagact attgatagtt
    16381 taaaggcgaa ggatagagaa atagtcgtgc tagaaaactc atacattcta ccgcaagtag
    16441 aaacgacaat ggagggaaag ttatgaaacc taaagatctc gattacatgc cgctgaacgg
    16501 cacggagtat ttcgacggtt caaaatgggt atgcccaatg aacttcgaga aggcaatcaa
    16561 aggtgaaaat gccagagaga gtgtatatat acacgatgtc acgctaagag acggagagca
    16621 gacctgtgga ttgacctgga acgaagacca gagggtgcgg ataggggtag ctctcaacga
    16681 actcggagtc aaatctatcg aagtcggcat gccggtggtc tccgacgaga acaaaagggc
    16741 aatcagaagg ctcgtggata tgaatctcac ctccgagata gtgcctttcg cgagggcgat
    16801 gaagaaggat atcgacgcct ctctggagtg cggcgcaaag agagtggtgg tggaacacgc
    16861 cataaatcct tacgacaatg aatacgtgta cggagtatcg gccgaaaagg ttatcgatag
    16921 agtcgtcacg actatcggat atgcgaaaga gcagggaatg gtaccgacct tcatgggctg
    16981 ggacacgacc cgttcgactt tcgactacgt cttcaaagtt ttcacagaag tggccagaca
    17041 ggccaaaccg gagagcatag tcttcgtaga cagtttcggc gtggcctccc cgttcaccat
    17101 agagttcgtc ttcaccgaac tcagaaaggc gataccggat attcctctgg agttccatgt
    17161 tcacaacgaa ttcggactgg ccatgggctc ggtctttgcc gccgtaaggg ccggagccgc
    17221 gggtatccac agttcgatca acggtctggg cgagagaacg ggaaacgtcg cgacggagga
    17281 agtggcggcc ggtctgaaat tgctcatggg tatcgatacc ggggtaaacc tcgaaaagat
    17341 cgcctacact tcgagactgg tggaggacat ctcgaaaatt cccgtcgcca acaacaaacc
    17401 tgtagtcggc aagaggctct tctgggtcga gtccggcata gtcgttgacg ccatagacaa
    17461 gttgaacgag gccgggatcg aaccggcgat gacaccgtat cttccttatc tggtggggat
    17521 gggtgagatt caagttaagc ttggagcctt cagcggtcag gcgagtatca agtactggtt
    17581 gaagagactg ggaataacct gcaacgacga gcagctcgag gaattgacgg agatagtccg
    17641 gaaggaagga agaatcagaa acaccgttct ggagatcgaa gaattcaaga agatcgtgag
    17701 tgattactta ggatgagttg ctatatactc gttatcaacc cgggctcgac atcgacgaaa
    17761 atagccgtct tcgacggcga gagggaggtt ttcagcaaga cgctgacact cgaggagaat
    17821 cagctcgatc tcccgctctt tccagaacag tatcttataa ggaagagaca gatactccgc
    17881 gctctggccg attcggggat cgaaccaaac cagctaagcg ccattgcggc cagaggtgga
    17941 aggctcaggc ctttgagttc gggagtttac agaataaacg atcggatgtt gaaagacgcg
    18001 agggcgggtc ttcagggcgt ccatccggcc aacaccgcgg ttgtgatcgc ctcggaaata
    18061 gctttcgaga accgtataga ggccttcacc gtcgatccaa taagcgtcga tgaaatgtca
    18121 gaatgggcaa agatcacggg tatcagggat ataacgcgca actcgctatc gcacgctttg
    18181 aacatgaagg cgatagccag gagagccgct tctcagctgg gaaaaagtta cgaagaggca
    18241 aagataatag tggcccatct cgggggaggc tcctccgtga gcgcccacct tcagggaaga
    18301 atggtggatc tttacaacag cgacaaagaa ggacctttcg ccgtggaaag ggccggcgcc
    18361 cttcccaccc acgaactggc cgatttcgcc cgctcgcgcg acgattacct tcatcatctg
    18421 gcgcatgccg gtggccttta ttcgcatttt cagaccagag atgtggaaaa aatcgtggag
    18481 atggctcgcg gaggcgatag ccgcgcagtt gtcgttcttc aggcttacat ctataatatt
    18541 gcgaagtaca ttttcagttt gctggccgtt ttcggcgggt accccgatgc tttagccctc
    18601 accggtggcg tcgtgaaatc ggaattcgta agggcggccc tgctagaaaa gctcggaaag
    18661 ggattcacgg tctttctctt tccaggcgaa ttcgaaatgg agagtctcgc gtcgggggtt
    18721 tacagggtga taaccggtcg ggagaaggcg ctagaatatt gaggtggtac gatgcagatt
    18781 aactcgcttt cacagatagt atcaaaggcc aggcagagcg gtggaaaggt tgaagtgctt
    18841 tgtgtccagc catacgacga ctctaccctg aaggctctcg gtagtttgag gtcgtgggga
    18901 gggggtataa aactcactct gtttggaaag agagaaaaga tagtatccag cgagtattgc
    18961 aggggcctgg aagatgtcga aataatcgag ggcgataccg aagagatcaa caccgccagg
    19021 gcgatagaaa cgataagggc gaacaagccg gccgttctca tgaaggggct ggtgaatacc
    19081 gccgtctttc tgagagttct tttcagaagt gaaagcgtga aagcgtcggg tcgtctcgta
    19141 agccacgttt cctgtctcga agtacccggg tttgaaagac ttctcttcat aaccgacggc
    19201 acggtcaacg tgaatccaga tctgaaaacg aagataggca tagtggaaaa cgtttgtgag
    19261 ttcgtcagat ctcttggctt tgaaaaacca aagatagcgg ttatagggat aaacgagaga
    19321 atcacccagt ccaatcagga tctgatagac ggagccgtga tcgcaaaaat gtcggaaaga
    19381 ggccatttcg gcaattgcta catcgatggt cccatgcctc tggataccgc gcttagcaaa
    19441 gaggcggccg tcaagaaaat gattctctct ccggtagggg gagaggccga tgtactggtc
    19501 tgctcgaacc tcgagtcggc cgcgaatctc ataaagggac tggttcatct ggctcacagt
    19561 aagacagccg gattgcttct cggtgccgga ttccccgtag tactgacctc caggagcgat
    19621 tcgagcgatt cgaaggagat ctcgctggcg atggccgtcc tgaacgcgac cgggggagtg
    19681 tgaggcttca tgagactggc cttcatgatc gcaaacgaag atgttccatc cggagccagg
    19741 gttacgggtc tcaccggttc gctagaagaa aatctcgggc agctcaagaa agccggattc
    19801 gactttttcg agctgatgat agccgatccg gcccgtgtag atgtcgagaa actccgtttc
    19861 ctggagagaa aaacggaaac cgggatctcc tttctttgta ccggcgagat ggccggaacg
    19921 atggggatgg ccctcaacag tccggagagt tcgcaaagag agagggctct atctggattg
    19981 atagatgcgg cagagaaggc gaaggcgctg ggcgtcaacc ttaatatcgg tagattgagg
    20041 ggaactgtgt gggaagacgg accggaaaag tcgctggagc gattaaagag agccctgagg
    20101 gttgtagatg aatatgtgtc caataacctg ccgggtctgt cgatcctcat agagcctttg
    20161 agaagagata tctgtcctct gttgaacagt tgccgggaaa catacgagtt catttcttcg
    20221 agcgggttga agaacttcgc aatacttctc gactcggatc acttcgatca gcgatccgac
    20281 ccggagtatg tccaagaaca gataggaacc ataaaacacg ttcatatagc agacaccatg
    20341 catgagccgc ccggaaatgg aaagatagat ttcgagaagt tcctgagcct gctcttcaag
    20401 gctggttaca cgggagatat cagcgtggaa gtcttttgcg gcgcagaaca gtacgaaacc
    20461 ttgagagata gctacaggtt tctgggcgag cattcccggt tctgggaggg attacgatga
    20521 ctggatccga cggcagccgt ttccatggaa ttcaacaaca ggtttacaga tctattttga
    20581 aaagtatagt cgaacttgag ataccgcccg gcaaaaaatt aactatcggc cgtctcaaaa
    20641 ccatctacgg tgtctcgtca acacctgttc gaggcgctct ctacaagttg aaggaggacg
    20701 gtttgctaat gctggggccg tcgaagagtt tctatgtcaa ggagataaac gaggaggaag
    20761 tgagaaagct tttctacgta aggatcgaac tcgaaaagct ggcgctgaga gaatcaatcg
    20821 actcggttga tatgacggcc atttcaaatc tgataataag aatgcggaag cacctttcca
    20881 accgcgaaga aactatggaa aacgttcctt acgacatcga ttacaaactc cacaggctaa
    20941 tactggataa ttgtggcaac agctatctca tagacataat gcagaagata tcgatactca
    21001 tcaccaggat gagaaacgtc atcaagtact atttgccaca gcagcagaag gactggatca
    21061 tatgcgagat ggaagaacac ctggagatag cggaggggat tctggagggc aacttaaaaa
    21121 aagctgaaat ggctctcgac agtcatttga agcactcaat ggatataatc tgctcgatcc
    21181 tcgggtcttc gaaagttgat tacaagacgg caaccagctt tttagaattg gaggcaaagg
    21241 tatgaagcaa gcgattttgc tttctcaaaa cgataacgtg gcgactcttc tggaaagagc
    21301 ggagccgggt gaaacgatcg agataaaagg taccggtcca gcgggaaaga tcgtttccag
    21361 gaactctata ccttccggtc acaaagtggc cgtgaaagac atgatgcccg gagacacggt
    21421 gataaagtac aacaacagga ttggaatcgc gaccgacgaa atatatgcgg gacagcacgt
    21481 tcacgtccac aacgtcaaaa gtgccagaac ggaatccggt aaaggaggtg aacaggctta
    21541 agccgttact atatgcagaa aactataaag ggttacataa gaaaggatgg aagtgtgggt
    21601 ttccgaaact acgtgttgat acttccgagc gttatctgtt catcgaaggt cgccgagagg
    21661 atagcctcgt cggtacctgg ctgcgcggtc gccaggcaca accagggctg tgcgcaactg
    21721 ggagaggact ttcaccagac ccgaagaacg ctaataaaca ccgccctcaa cccgaacgtg
    21781 gcggcagtga tcgttatagg cctgggctgt gaaagggttt cgccccacga actgagagac
    21841 ctgatcgccc aggcgggcaa accggccgag ctaataatgg tacaggaggt cggcaacggt
    21901 gaagccataa gattggggag ggaaaaggcc agggatttcg tggccgaggt ttcgaagata
    21961 gagcgacaga gtgttccgct ttcggcactc acgatcggtg tcgaatgcgg cggttcggat
    22021 ttttcgtcgg gactgtccgc aaatcccgta gtcggacagg tggccgacct ggtctggaaa
    22081 aacggcggtc gggtgattct ctcggaaacc accgagctcg tgggggccga acatctcctt
    22141 ttcgaacgca tgagcgacga ctctcagaag gaaagattca cccgcatgct cgagaggatg
    22201 atcaacgaga gcagaaacaa cagccgcgac atggtcgata gagaaaatat tccaaacaac
    22261 atatcgccgg gaaacgtcag gggagggctt accactctgg aagaaaaatc tctgggagcc
    22321 atgataaaag gcggcaaagt gcctatcgtg ggagtcaaag agtacgggga gatcatagaa
    22381 gcgactcccg gtctttatct aatggactct cccgggtacg atgtggagtc cgtgacggga
    22441 atggtcgccg gtggcgccag catcatactc ttcactacgg gccagggaac gccgacggga
    22501 aatccgatcg ctccggtgat aaaggttacc ggtaatcgcg acacggccat gaaaatgaag
    22561 gacagcatcg acttcgattg cagcgcggtg atttccggag aagagactct tgaaaagagc
    22621 ggagaaaggc ttttcgatat ggtgatcgac gtttccaacg gagcggaaac caggtcggaa
    22681 ctcctcggac aggacgacta ttctatatgg aacgtcggta taaagctatg attcccggta
    22741 aagacgagct ggagaggatc tttcttgaaa tgtgtaaata cgatactacc aatcctcccg
    22801 gcaacgaaga atctctggct ctgttcataa aggaaacgct ggggagttac ggtgtaaact
    22861 gcacgattca gagagtcggc gacggacggg tgaatcttct cgcgaaaatc gacaggaacg
    22921 gcgggctgcc ttatctgctt ttcgccggac acatggacgt ggttccggcc gcttctggat
    22981 ggacgggcga tcccttcaag cccgtcgttg agaatggaag ggtgtatgcc cgtggatcgg
    23041 ccgatatgaa ggccggcctc tcctccatga tggcggcttt catcgatctt tcctgcgatg
    23101 aacgattcgg gggaaatctg gcctttctcg cgacgtgtga cgaagaagtt ggctgcagcg
    23161 gagtgaaaag atttctcgaa gaaaacacgc tcgaaataac cggcgcactg atcggcgaac
    23221 ccacgtcgat gagactggcg agtggagaaa agggagcggt ctggagcgaa ctgaatttca
    23281 aaggtaaatc ggcgcacggt tcacagcctc aagagggaat aaacgcggta ctccttctta
    23341 ttgacgccta tcaaaggatt gccggtgaac taggtggcat agaaggcctg acggttagtc
    23401 tgaacaaggt atcgggaggt tccaaggaga acaccgttcc ggattccgcc agctgcgttc
    23461 tggatatacg ttttatgagc aacatctcgc ccccggaagt caccggagtc atagataaag
    23521 tcctctcgcg gttcgaaggc gccggtcaga aggttctact aacacgcaac cctttcagct
    23581 cctccggcag gttgagcgat tgtgtccgct ccaccctcgt agagattggg agagagccgg
    23641 aaccactgac aatgacatac ttcaccgacg gagccttcat agccccctcc ggtatcgaga
    23701 ccgtgatact cggaccggga gcggccagca tggcccacaa ggccaacgaa tacgtcgagt
    23761 tggaagagat ccatatcgca agagagattt atgggaagat cgtcagaaat ttctttcaag
    23821 gatgtgtctg agatggatat gacagtaata gacagcccgt cgctgagaaa tctatgtgtg
    23881 gatattctgg ttgccgaagg tttcgggcag gatgtttcgg ccgatatcgt cgatgtgctt
    23941 ctggaggcag acctcagggg aataccctcc cacggggtcg cgagactcag gcgatacata
    24001 gacgagagaa acgccggcca cataaaactg aatgaaacgc cttcggtaaa gtacgaaacc
    24061 cccctctcgg ccgtggtgga tggcagggga gggcccgggc agagcgtttc aaaatgggct
    24121 atgaatctgg ccatagagaa ggccggaaat aacctggcgg gattcgtttc cgtacgaaac
    24181 tctaaccatt acggcataac ggcctactat tcagagatgg cgctcaaaag agatatgata
    24241 gggatagcca tgaccaacag ctaccctctg gtggtgccga cgttcggaag agaggccgtt
    24301 ctcgggacta atcccttttc catagccatc cccggttcca ggcatccctt catactggat
    24361 atggcgacga gcgtagtgac caggggaaaa ctcgaagtgt acgatagact ggcaaaagta
    24421 attcccggag gctgggccgt agatagcctg ggcgccgata caaactcgcc aggagaggtt
    24481 cttgccaatt tcaacaaccg gagaccgggc ggaattcttc cgctgggcgg ttccggagag
    24541 gagttcggtg gtcataaggg attcgggctc tctctgctgg tggatctggt gtcggccggg
    24601 ctctctctcg gttcgtggag cgctctgact tattcggaag gagtcgccgg cgtctgtcac
    24661 ttcttcggcg ccataaatct gaggctattt ggagaaccgg aaagcctgaa gcggcaagtt
    24721 caatcgataa tcgatggcgt cgtgtcaagc gggaaggcct gcggaagcga gaagatctac
    24781 taccacggag aaaaggaaga gctctcgaga atatcttccc tttccaaggg agttactctc
    24841 gatccgaaga cggtcagaaa cctggtctca ctggcagaaa agaacaacct cccgataccc
    24901 gaccaactgc tgcaataaac ttaaaacact acctgaaata taaaaaacgg cctgaagtct
    24961 agacttcgga tgtgatggta tgccgctata attccggcga agtcggcgat tagatcttcg
    25021 atctcggccg tccctctgcg cgaggtgaaa tcccagacct ccttgcctat tccaagggcc
    25081 gatgtgaaaa taaaaccgcc cacgtcgccg agcagaaagc tcgacaaacc gtaggcggta
    25141 taacttacag aataatgaag gaatttgtcg taacccgtta ccgctaaact cacacataga
    25201 aaagaaagga acagaaaaaa acaaaatctc ttaatcaaaa aagctcttta gctcctgact
    25261 agaatgaata aagaacgccg agacctacag agaaaaactc tggaagaggc cagtagtctc
    25321 cgccggggaa gtttccttcc tggcgcgttg cgtatttgta aaccctgtag ccccttgcgt
    25381 aaccgttgag tgttaccttt tcagtaaccg gcgccattag ctggaagccg ccacctattg
    25441 ccagatgcgg tgcaccgaga tcgagcggtc cgatcatcca gcccggttcg ccgaaatttg
    25501 aatccattac cgaggcaaca ctgacgagaa gccttaactt caaattgaaa atctcgaaaa
    25561 tctggaaata aatgtctgca ttcaagagac cgatcttgtt agttggatat cttacagcag
    25621 cctgagcact cagcgttgta cctatctttt cagtgaaagg aagctgaaga gacagatcca
    25681 aagccccggc accccatctt tcgggttgat agattggatt ggtggcgttg ataccgaagg
    25741 ctccaccgat aagcgcttta ggtgcagaga aacccataat aaccgcagaa aggagcagag
    25801 ctaccaaagc gactttcttc ataaatacac ctcccctaaa ttattgcaaa gcttttggca
    25861 ttattggcag tataagtcag attgattata ccacaacaac atggacgttc aaagccgttg
    25921 atgcagcctg gtcgcgtgct gaaaaatctt tctctctctc gatcttcaaa tatcgacctg
    25981 ttgtctcaat tttctctcga catctctacg tgctacatct tctcgcttat cgaaaagcct
    26041 ctttcctctg gccatagcta tttcaacctt ggccctgccg ctatcgttga aatagatctt
    26101 tgtgggaatt atggtgagcc ccttttcttt ggttttctga tccagtctca gtatctcctt
    26161 cctgtgcatc aacaaccttc tctttcttct gggatcgtgg ttccagagat tcccgtgaga
    26221 atactggctg atgttacagt ctatgagaaa caactctccg ttctctattt tacagtaggc
    26281 gtccacgagc gaagcccctc caagtctgag agacttcact tccgttccca tcaattccat
    26341 accggcctca aaactgtcca gcagatggta ctggaacctc gccttcttgt tagttactac
    26401 gatcttcacg tgactccctc cactcgactt tgcgaaatag attgtatcac atctgtcggc
    26461 attaacaaaa agggatctct ggagatccct cctgtgttcg ttcaacttag aaaactctgc
    26521 caggcatatt ttaacccgtg cgtctgtctt gttccataat gcgagttgac aaatgttttc
    26581 gaatcggagc tatttcaaaa gcacgatatc cggtctcctg actgtctggc cgtcgatttc
    26641 gaaggtcaag gccggccatg tgctcgatcg ggggtaggag gcgattcgat tttgcccgtc
    26701 cagcaataag actgtgtctt ccgacttggt acccctgatc gtcgggttcc aggcgaggga
    26761 gtttccgctt ttcagcacaa tggaagtgta tgggtcggcc accacttctc ttggagagta
    26821 gccggcaaga cctccctggt gatggaacct ccactctccc ggagctccct gcgaggcgta
    26881 ggcctccttt atcttttcga aaacatcgga aagttttttc ccgggtctgg aagagacgat
    26941 cgccaccgaa tccacgtagc agttacacct gtgctgctct ctaattcctt cgacttcttc
    27001 gaacatgaaa gagcgagaag aggagatgat caggccgcgt cttctggcac aaatactcac
    27061 gaagcctctt cttccgagcc tcgtgtttct cgataagttg tgtctgtaag tcaaggtact
    27121 ctcctcggaa aaggccagag tgagtaccgg ctcgatatct ctcttcgcca tttcccggta
    27181 catgattccc tgaacttcga gctcggtcat ctcaggtacg aacgatggaa ccacatccga
    27241 gaagatttcg tcgcattccc tcccaagatc cgaatagaac cccacttcgt agtcgctcaa
    27301 gacgctcctg agagatacca gatcgacggt tccagtatcg tatctgccgg tgtcggagat
    27361 gacttttccc ttcactatcg agtcgatcgc tctcttttcg gcggaggtac gctcaaacca
    27421 gtcgtactct accacttcaa gttcccgctg tatttcttcg ggtagctcca ggtctttgag
    27481 tctttgaagc tctatgttgt ttatgagcag ataaacaccg tcccgcgtca ccagaataga
    27541 ggcctctcct tcggtcgcgt tgagtgttat gtggctgcgc gcaccgaacg tgaaccagga
    27601 gaagttgttg cacttgctta tcagaagagc atctgcccca ttctcttcaa ataaagacct
    27661 taccttctct actctttctt tgaaaagctc taaaagcatc tgtgcacctc acacgttttt
    27721 agttactata tgcctttcat cgatgcttcc aggaagcgat accggacggt tcgatgacgt
    27781 tcgtctggat tccagtacgt ggcttttcca tccaactctc cacagtgttt ttccatttca
    27841 aataatgatc ggtcttcttg tgggccgcag ccgcctcttc cgattcatag gcttcgtaaa
    27901 gaagaaagtg attcggtttt gatctgtcct gcaggaagtc gaatctgaga ttcccctctt
    27961 ccataatgga gctcttatgg ttgagtgtag tagcttcaat gaattcatct atcctttcgc
    28021 tcttgacata gatatccact accgttacta tcatcgccaa cacccccgag aatttcgaaa
    28081 ttcccagtga aacaacagac cgatctacga ctaaacagag gtccaataac cgttcagccc
    28141 ttcgaaagag tatcaagagt cgttcaccgt ttaaaacatc taaaatttaa agccttcctc
    28201 gaagcacttg aaacaggtct tcactacatc gctgtcgtaa aagctaccgg ctttatcgtc
    28261 gatttcattc aaggcgactt ctattccata atcgtccctg taagttctgt agaagctcat
    28321 ggccgacacg acctcggcca cggccagaat tctccctccg atgctgatct catcgccctt
    28381 tagtcccagc ggatatcccg aaccgtcgag cctttcgtgg tgctccagta tagccctggc
    28441 gatcgtacgc gggaagggaa ctttctttac gagttcatat cccacgagag gatgttcatg
    28501 cagaagctgt gattcgaacc aggtgagctt tcctggtttg ccgatcatgt ccgaaaagag
    28561 agacatcatg ccgatatcgt gaatctttcc ggccgtttcc agttctccgg ccaatttact
    28621 gtcattcatc tttctggcta ttgcagaggc aagtctcgag acgttttcgc aatgatcttc
    28681 ggcataaaat cccctgattc taaacatttc caaagccatg gtcgccgttc ctctgtataa
    28741 ttgcctacat tcgtcgctct cgcaattatt cctgtaagcg tctttgaaat aatcgtagtt
    28801 tggaacgaag ctgtcggtat cgaaagcgaa cgggtgaagg ctggaaatct ggagaagcat
    28861 ccttttgttt acgattgatg tatcgtatat acaacaggca tcgagaggat tttcgctgat
    28921 aaactcgtcc atcttcgatt cgtactcgac catcttggct gcatcaaagc tttcatcgaa
    28981 gatccacgag ccttctccgg ccacgcagag tccttcgaat ccgctgtcaa gcgcttctcg
    29041 tgttttttct tcgagctcct tcgccattct ttccagatct agaacaccat ctctcgagta
    29101 ggtatctctc acgctggcga cttcaatcca gccagaacgg cgtaactctt cgagattcat
    29161 cccgtaaggt tctatcgccc tctggagatc aactagagtt agcctgtcat aaatgaaaag
    29221 cagtttcttc tcctgactgt atccgttgaa gcagaaactg ccaagagccc tttcgatctc
    29281 agacggagcg ttgtaaagaa taaccctgtg tgagccgact tcagttgaat gagactgtcc
    29341 atgtttcata ttctaaaatc aaccccttga aataataaga atgcttttaa gtgaattcta
    29401 ccacagtgat tgttgaacaa tgcaatattg agtctaatac gacattatta tcaacaaaca
    29461 attataatta acataaattt ctaaaaaaat attcccaaca gctcaactta tgcatttcga
    29521 aagaagcgag gggaggactt aaaacctgta tccagagcgg tggtgggtgg tgcatagtgg
    29581 ttagcgggtg gtgaatacgg tttttaccaa gaatctggac gcgtgacgtc gaaacaaccg
    29641 ctctttcgaa ggacggcttc taatgctctt cttgcatctc gcctctaccc ggctcttagt
    29701 tttatctcaa aggtgctacc tttgtctggc tcgcttacga gatttatctc ccctttcatg
    29761 gttcccacaa gctctctggc gatggcgagt cccattccgc ttccggtgct gttgtgagat
    29821 ctttcggatt tgtagaacct ttcaaagatt ttctcatgct ccacaagttc tatgcccgga
    29881 ccgttgtcta tcacctgtat gtaaagatat ccgtccctca gaaagctttt aagaacaact
    29941 ctggatccat cgtagctgaa cttcaccgcg ttttcaagaa gattgccaag cgatgtggtg
    30001 agcggctcta tcatcacggt cgacacgagg ttgtcttcac attctatttc tatctctata
    30061 cctctcggat cggagagcgg tctgtaagac aaagcgatgt cctccagcag tctctttatg
    30121 ttcaccggtt cggctttcgc cttaatttcc tgcgagctct gtattctcgc catttttatg
    30181 agctggttca cgaggttttg catgttcctg gtcgcagtgt cgatcatgcc gatcttactc
    30241 gaaacctcgg gatcgagctc cttctctcca agcatatcta ggccgagtct tattatcgag
    30301 agcggtgttt tgagctcgtg cgaggcatcg gccgtaaagc gctgaagtct ctggtagctc
    30361 tccttaaagg gatttagaac gtatccggcc agtgccagac cgattatcca cgatatcgcg
    30421 gccgtcagca aaacaagact caggagcgcc cagagcaggt ttctcatgat gttgctcagc
    30481 tcgctaaccg gtttgcctac cctcatgaac acgaccgact gaccggcaag gctggtgagc
    30541 gatctggtca gaagcctgta ttcgctttcc ctgtcggagt aatcgaccac cataccgttt
    30601 gcgaagcctt ctttgagagg aattacactg agcagtctcc ctcccagctg gagaatgctt
    30661 tcacccgagg tgctgaggaa ctgcaccgga atcg
//
LOCUS       DAOOLY010000002        28026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_1495_length_28026_cov_5.272067, whole genome shotgun sequence.
ACCESSION   DAOOLY010000002 DAOOLY010000000
VERSION     DAOOLY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 28026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28026
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_28026_cov_5.272067"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5642
                     /locus_tag="PLD15_00180"
     CDS             <1..>5642
                     /locus_tag="PLD15_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62339.1"
                     /translation="NTGNIETYVYLKAEQVFESDLEEETFFKVASSPYPVLRVVGIFH
                     DRNGNGRIDDGEPKIEGIDLPVEASEKIIVRVMVEDKGSVISTGFVNVVGDDMMGNVD
                     EDNIAMIEIKEGEILTFEKTQLNETIQPGGTQRFTLRGKNLSDYSLDTVTIREYIDFN
                     GYNVEGIADLDSIEANLPYTPGYFDGKEWSGTRPGSDEIVKGFELTFSNVLAGQEIVV
                     VFDVFFPPTASPGVRFNAAELLYSRAGSFWEGRTNTVEFNIPVSTVPFLGPLGKPQAA
                     EMTEEDTTVSTVTVYGGDSVSFPHTLKNGGNRSGVMDLEIESANFDAEGWNFVFKDSS
                     GEPLVDTDGSGLVDAGLVGPGEERDVVLEVSTPSQASGDNAGKSFRFIPRTVMNGESN
                     RTIDIVPFIYESGKLSLEKAVTSATLLLPGESVRFSIIVKNESSSASKAVVITDEIST
                     MLEEPFDISVSKEATVTYDGLSRILTVEIDSLDPFESVEISFSCKTSLSLEEGTVVKN
                     VAKLSSGAIGETSNDVSVRIFQGRLEVVKSASPSVVELGSEITYTVEVFNPSSIATVT
                     ELRIEDRIPAGTVYVESSATIDGEPIEPVVEEGVLHFTGLKELGPGKRLTLLYRLRLV
                     EYTGDSLQNRVLAAGTVLSEEYSAYVTTAPSVVDVKILKPMNLAGGISGRLYVDENGN
                     GYYDEDDTAPLPVRLLLQDGSFAVSDREGLFHFDRLSPGSHVLKIDIDTTPYKLAALQ
                     DSRSLKDGRSYMLTILPGMYTIVDIPLVHREKAIETITFKEPSTLKGLEGSSAILSAF
                     YEGYSAGDSSDGYIVFPENGRDFTGTDRITVEVAVPPESLYRLSVNGSQIPDTQIGQK
                     ANDLEGRWLFARYFNVKLTPGANRIRVEWQDLTEKGSAEVQVYLSGEPVEIRVSSEPR
                     VLIADGITEAVLTISLVDERGIPASVGGTVIVEGLDPDSVLEPGENWDGKRLKLENGV
                     ARIKLKPFFKAGTVEFTASYAELSREVRLEYRVESRPPIVTGFGRLQYNLHSKSFDYG
                     GGIFGRFNLGDGLVTLRLGEKAYGDYEAYVTHGDASTSGTLAPSSTWYFFRYEIGLFN
                     LHLGDYIFRSEGGQGFASQGTGLSSEYSGDKLSYRLFLNPSYRETRKEELRGQGVRGP
                     YYLNGTPIKNSETVFLVSRDSQGEELTRKLMKRGTDYTLYPNGLLIFTRPVPYYDIDL
                     NEIFIEATYSVDSDLPGEIDVMATLSYREKGWKYSFTGLVKGLLEGYRFASIEVSAPP
                     IASFQPELNVYLSSKDGEIGFRAQAASTMKNSFVSGKLEVHIDRQFLSPNSSRVSSGW
                     GIGLELAPVFPKVRLLSGYNYDEKNELGTLSAELLTDVKTGRIFTEISLKDTLIHRQA
                     GFENEARLTLKPSFSVERVKINGEVYAGLKGLSPIAGLSLRVDLGLSESLFTGGDLSF
                     DYAGSDRLKLSLTPFILKKLGEISLVAREKIEFLPDLRFTTVIGFGYTLDNGKFDLEA
                     TLSDKVSLGAGYRATFSPEIVNVDLLIQGRYTVAGTGTEDFLSAHLGIENREIEHLKL
                     RLDTDLKIGLDFKLSTLLIESTGEYLGFESLKPAYRVLFAQDFRLQNSKFEVQTGLAY
                     RPDWRTPLTVLFEGAYYMNRQKEKIDSEGRLTLDVAYWLDRTVNLSLTGEIYLKNALL
                     YTVGGLQVEKHFFDLFSLLGGAYLVSDNAGNLYNRFTVEAGFSPLPDTQLYAGYGWGT
                     LLESFTGNLQKSGFYFGVRVKFDDGWFFKNSDRGSLSLLFFRDRDFDGQIDPDETPVR
                     ARVKIGEQEYESDENGEVRVNLPAGLYTVELLEYPGDLISLLDGSADLSVTGYGTRQF
                     NWPFMESPAFLDIRVFVDSNSSGKLDGDEKPLQSFSLSV"
     assembly_gap    5643..5742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5743..6210
                     /locus_tag="PLD15_00185"
     CDS             <5743..6210
                     /locus_tag="PLD15_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62340.1"
                     /translation="DEPLLDASGILAIGGKPFRIGSRTVLEGVPSGRSPASLTMDRGF
                     EKLYSRITTIDTVEVAETGDTVLEIGFAEKSSLMISFLDEKGDYFYDVVTVKIDGIEF
                     QAFGIIELVGLVFGDHLVEVTGLPKGFKCDRYLYEIWLEPGGSGEIEVVIGKE"
     gene            6215..6646
                     /locus_tag="PLD15_00190"
     CDS             6215..6646
                     /locus_tag="PLD15_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62341.1"
                     /translation="MDFFRALNEIAFYLSFVLWAGGGVTVSVFLIPLRDPNTPPQVQG
                     FLYSLWENLRIVAMAVSIIMLGTSMTRLIIDPVVNVFWTVICFATFSLTVFSWYMADR
                     LIRMGASDPRSMLEVEKRNFGLYLFYADACIVLTVIAAALM"
     gene            6730..7452
                     /locus_tag="PLD15_00195"
     CDS             6730..7452
                     /locus_tag="PLD15_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62342.1"
                     /translation="MAGREYSFKQKIIETLHDITVRDMSVGIDGIAYVSISVPSLEVS
                     GQSSIDGYFKAGYYPSSKGRPAHLGISLKVDDKRHEGMTLSLEATAVLQKWYEKAYAD
                     IKRRFKSIVDRVVDDELPVIFSLAGDDFPKFTPSLQIEDEDLKHESYKVLKHAIVRFF
                     KLHGAERYAKVARADDYLVESIPPPPVSDDRLPPTAFDIEHERDLYDIYGTGPGSVFS
                     FKMKLSDILNPGLKQLLEREEY"
     assembly_gap    7536..7635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7710..9509)
                     /locus_tag="PLD15_00200"
     CDS             complement(7710..9509)
                     /locus_tag="PLD15_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB62343.1"
                     /translation="MSKVGHDYGQDFLFDDVSTSIDKKDKIILIGKNGCGKSTLMKII
                     AGELKPSEGEIFHSTSVKIGYQIQNRIPDSEITLIDYYMSDKSLLVPDTQEYYSFERR
                     VRSTLVGLEFSEEDWNRKLNTFSGGELTRISLGKLFLVDYDLLLLDEPTNHLDLESTE
                     WLVGFLKSYQGALVVVTHDRYLIRKVGNRFWELNGGSMWDFPGDYDKYLFDREILMKS
                     GTRARENLEKEIERLDAVAKRYRLWGQEKFIKQAINKEKQRDRLKEQLESVDLPDEEV
                     RAPRFRLPQPDRTGYIVLKVEELSFGFDGKELFRNAGLEVHRREKLGLLGPNGSGKTT
                     LLKMITQDLEKTSGEVEWGHNVRWGYLSQLTEDLNLNNDVLTEIWQLMPGQPDYEVRK
                     YIGRFGFPGEDVFKPISSLSGGEKTKLALAKLILSKPNVLVMDEPTNNLDIWSIESLE
                     DVLREYEGCIILVSHDREFVQNICDHFVMIDRLKFRSVRSVQEYLNRNRPTRESNDNE
                     ASRLSFQEKRKLTNRRKSLCERYDLLHNEEEELSRKIEGAQLKMGLYVTNYEKLQEFQ
                     ATIESSEEKILELLEEKENLERELEELDRLLGE"
     gene            9662..10246
                     /locus_tag="PLD15_00205"
     CDS             9662..10246
                     /locus_tag="PLD15_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62344.1"
                     /translation="MKLGVSLLIFVTTFLVMLYIWGFYFFKSPGQVFEEYERALVEYA
                     HQMRIQEEAGLKAGLGARELSNLREAAIVDLEGDMKRIVADFEKDWSAMRRIYLEYAR
                     RSGSWFTEGLTGVIVTQDNPDYHTLLALYNLIDDPDSYCLLFIPETNGSRVFNEFSGA
                     RLYYLQKTLWGYRIEWSKSLIDLLLGKDITSIAV"
     gene            10276..10779
                     /locus_tag="PLD15_00210"
     CDS             10276..10779
                     /locus_tag="PLD15_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62345.1"
                     /translation="MFLKRLVAVLLILIVTATLVASPEMLARQTFAAILGGFAGSVSG
                     AVIGTVLGGLINPTGWGDLVGLILGFIVGAPVGSSLTTISWSVDRLEGSVLLSIAGSI
                     GGVAFPYALSGLIANVFPANDFNPSGIVLMISLPLYSAFGAVLGYNYPRLISGDLVLE
                     DLSTTVK"
     gene            10912..12219
                     /locus_tag="PLD15_00215"
     CDS             10912..12219
                     /locus_tag="PLD15_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62346.1"
                     /translation="MFWKEKRVEKKLEKEIGRKERELAGSTDPLQRGSLLDELALLKE
                     EKGDLCGAISALLDKFKEDRKVETLKKLRPILEKHSEFDLRKLEERLFESSENLDRTT
                     ENDLLKEIYSIDGKLALQLSLRFFEISREYACGLSAIDIMERILSENGREHDVSIVET
                     VEEYHRLVNELVEISPDRGEQILARAAGFSKEKLGNLEPFLDFSLRRLGDESSLEVRS
                     GIVDSVIEKFDNLATVFEKREGELLNRLEVLLSPEMECKSVDLLLEHMIETARSINDS
                     REELYLDRKLKRADSQSLKSEAMAIERALKKGVPDISQWKRLAKLYRYVIDLSQDLEE
                     KILYIRKLGVLLRDKLEDYEGSLRVFEEGMKLKESDITLWLVKGMTLEKMAQNHPGSE
                     ATCDIWKAAYDHYLKIAERFDEQEIKLSAIEKCAIIQSEKLSV"
     gene            12352..14082
                     /locus_tag="PLD15_00220"
     CDS             12352..14082
                     /locus_tag="PLD15_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HPB62347.1"
                     /translation="MMNIKGEMIAMVEKLDGRKVKSLIELLSGFHRVRGSKDYHDSMM
                     AAIDYLKRSGMPSRRFKVFSYPADGKTVNGNVATTLAWEPIYGEMWLERPERFFITST
                     SISKVALVSGSAGSEGWKELSLVQFEGKKDYSNKAVLASDDPVKVFQQAVVEGGASCL
                     IFNYMRKSFEEIGRSLEKLPEMTNYLSIPYDREAADKKAVAFSITKEKYDLLAGAVAR
                     GGAVVGFRSEVKLTEGELEVLQVDATGVENGPGVIITAHLCHPSPGADDNASGAALAV
                     ELARILHQAEFPFPVKIALVPEYLGSVPYALQLKREKKLPLFTVNLDMVGADQDKTGS
                     TFILSKVPPYLPQKWGRILEYFITELMPRNGGYPLKRFGEIPFMAGSDHCVFTTLGIP
                     SPFLGHLPDRYYHSDFDTPSMMDRVELEWVGRSVLKTLEHIITPDPLAAAAAKGKMIG
                     ELHEILSRIAGREGAGDLLDLLVSNYEGDVLRGNFAEACGLSSSRPLEPTFESSLGLE
                     WMKIVPQELKNEVGPELLSMADFMAGGSLVIGSREAVELLTSIHYGVPIEKVRTLTGW
                     MIERSLLRSS"
     gene            14085..14780
                     /locus_tag="PLD15_00225"
     CDS             14085..14780
                     /locus_tag="PLD15_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62348.1"
                     /translation="MFLRFLWLSLLLTAVTVAAGSVGLQVDTGISMPWNGYLKNIETG
                     EKIEIIKRANFRAFFQTTIGYTFFEEHSIEAMVSPFSIESRLEQDGDEEPAEYTSRYV
                     QDTYRLRYRYLLPPLDRFYLQIGLHATLHMKELVIGGSREYTNEWTDFSFGPSALISL
                     SMNEIVSLVMEVEAGIGRDNGFLDIFTGLNLSGPFYFVRAGIRNLSTNGFSGGEALST
                     SFWNFGAQFRAVF"
     gene            complement(14784..15380)
                     /gene="xpt"
                     /locus_tag="PLD15_00230"
     CDS             complement(14784..15380)
                     /gene="xpt"
                     /locus_tag="PLD15_00230"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HPB62349.1"
                     /translation="MEIIEVSREEAVRSIQEAIIKKGSVLGGGILKVDSFLNHQIDPR
                     LIKTAGNLICDTFKDAEITKVLTVESSGIAPALATAECMGVPLLFARKRKPITMKRYI
                     TETAPSHTKGGIVELNVSLEFLGESDRVLIVDDFLASGQTIAALGRLVQKTGAKLCGF
                     AAVIEKTFEEGRNLLEEFGVPVLGLVRISSLEPLRFED"
     gene            complement(15430..16059)
                     /locus_tag="PLD15_00235"
     CDS             complement(15430..16059)
                     /locus_tag="PLD15_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPB62350.1"
                     /translation="MKNRRSMIDIGILSVSLYISFQIFADILSIKITTLPFLHLAIDG
                     GTVIYPFTFTLRDFVHKTWGKERSRQVVITAGILNLLMAVLFWLIGKMPADPTWNLQG
                     AYESILMPFWRITIASMIAEVFSELIDTEVFDRVYRRFGDIKAVLFSNGIALVIDSFI
                     FSFIAFAGVLPLITVLSITLSNIIVKMAVTLISLPTIKLVPRRDEYFKG"
     gene            16242..16685
                     /locus_tag="PLD15_00240"
     CDS             16242..16685
                     /locus_tag="PLD15_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB62351.1"
                     /translation="MVDDHTPEIRFDTDLEGILTYAVIGTRYCDRWIFVRHKLRRTWE
                     IPGGHIEEGETPLEAARRELQEETGARFFELYEICTYSVSKDGATTSGKLFYAEVTGL
                     EGLQPQSEIGEITLRVGLPARLTYPEIQPWLFKKIVKWERNFERS"
     gene            16672..17493
                     /locus_tag="PLD15_00245"
     CDS             16672..17493
                     /locus_tag="PLD15_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HPB62352.1"
                     /translation="MSEADAVSKSRSGPVTVISLRKDFENLGIKKGMRLLVHSSLSKL
                     GWVCGGPVAVIRAIEEVIGVDGTLVMPAHSGDLSEPSSWQNPPVPESWHGTIRKTMPP
                     FDRSATPTRGMGKIPETFRKMKAVVRSNHPQVSFVARGKDALYITDDHQLDFGMGEGS
                     PLARMYELRAFILLIGVSHSNNSSLHLAEYRAEYPSKEVCKDGAPVVEKGQRVWKEFS
                     DISLSTDDFEDIGKAFESESPSLVSRGRVGYSDGLLIPQRELVDFATVWMSENRK"
     gene            17585..18337
                     /gene="gpmA"
                     /locus_tag="PLD15_00250"
     CDS             17585..18337
                     /gene="gpmA"
                     /locus_tag="PLD15_00250"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPB62353.1"
                     /translation="MVMMKLVVVRHGESTWNKENRFTGWTDVDLSEKGKEEAKKAGEI
                     LKAEGFEFDLAYTSVLKRAIRTLWYVLDEMDLMWIPVIRDWRLNERHYGALQGLNKAE
                     TAARHGEEQVKIWRRSYDIQPPALEESDERFPGRDPRYGNLTADQLPKTECLKDTVAR
                     FLPLWKNEISSDIKSGKKVLVVAHGNSLRALVKYLDNIPDDEIVGLNIPTGIPLVYEL
                     DDDLKPIKHYYLGDPEEIAKAQQAVANQGKAK"
     gene            18467..19699
                     /locus_tag="PLD15_00255"
     CDS             18467..19699
                     /locus_tag="PLD15_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB62354.1"
                     /translation="MSIFTFLMDLARKEFKIDSLSGRDRLGFDRPAGLYIHIPFCSER
                     CDFCPYNKVKYDREAAAGYPQALERELELQGVKGFSSLYIGGGTPTLDMDLLEDVIDR
                     FSNSIDGELALEVHPADATVEKLERIKGTGIDFVSLGIQSFDDSTLQKMGRKRQSAAL
                     SREAIERVVLAGFDFVDVDLIFDFELESERTARDLEIAMDYGPHQISVYPMMRFTGTA
                     LAGTKNDPDRELQILEILEERARKRGYSRDTLWTFKKHGESKRYSSVSREFFLGLGTS
                     ASSFNGGQFTTNTFSLDEYYRYLDGDRIPVRRAIEMNTFQASLYYSFWALYGGSLDQE
                     RLRSLFGEPDIRLSGLLELARKTGYLRQEREKLVPTKKGYRELHMIEEWLTYNFIDVI
                     WTELREEAANRAHPGSVW"
     gene            complement(19678..20505)
                     /gene="ylqF"
                     /locus_tag="PLD15_00260"
     CDS             complement(19678..20505)
                     /gene="ylqF"
                     /locus_tag="PLD15_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HPB62355.1"
                     /translation="MYYPGHIQKAKRQIQENIKSVDAVVELLDARIPMTSRAYEAEKL
                     FHNKQRIVVLNKSDLADPQITQLWRQHFSSQGTDVIEASLMNSDARQFIIREVVPLVK
                     SKFFEKRFMVVGMPNVGKSTFINRLKGKKSLAAGNRPGITRGVQWINVSQQIAVLDTP
                     GILYTNLHSPHITTKLLAVGSLPYEQFDPMDAFERVLDLIVGRYGKESLFEYTGASFE
                     NSEDLIDKFCRKRGFLARQGQYDTTRGSHTFLRDVASGKAGRFSFEDPDTFYQTDPG"
     gene            20608..21459
                     /locus_tag="PLD15_00265"
     CDS             20608..21459
                     /locus_tag="PLD15_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HPB62356.1"
                     /translation="MKTNKDTVVKISVSGEVAHPLMRSPFRLEIDGTPRLFPGTGGIT
                     YNFALGDSAFKMTGDHVEPDVSTKNPDSDKNTAYVAYSCIGNSALVISGDAKGERGVV
                     TGKHGGINHVLIHFKEEVKEKMAIGDRIQVTGYGQGLVLEDYPGIRVYNIDPYLLERV
                     PVVESDGKIHFPVRAIVPGYLMGSGSGSGNPAGGDYDIITNDRSLVKRVGLDKLRIGD
                     FVALENHNDSFGLGGYMEGSVTIGVVVHGDCIITGHGPGVTVIMADGEGIIVPEVSEK
                     SNIVDFI"
     gene            21510..22523
                     /locus_tag="PLD15_00270"
     CDS             21510..22523
                     /locus_tag="PLD15_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPB62357.1"
                     /translation="MRDPGFGVWVARSTLLNPAGVEKMISKLHEISAGRAYVQVVGRG
                     DSQYSSRILPRAEELDGPFDGLSETIEMARGSGLKISAWMNVNLVWKFGDRPSSKDHV
                     VNSNPEWITCDERGLSMLEYSSCDIEEVFGPFIDPGIPQVRRFTAEIADELASYDVEE
                     VHLDFIRYPFRTFGYTQAALHEYRRWLRAVNLKDTEENFDRFRIESTGEQVRLIREKV
                     RSRGKKLSCAVFDDYSERAVKERLQPWLGWLEDGLIDSAVVMAYGREPDGVISRVENI
                     CNIHGSLKGIRVGLGAFNYAGRWDDFIGLVKRVRKMDPDEITFFALSSIDEELAGKLK
                     ALD"
     gene            22539..23648
                     /locus_tag="PLD15_00275"
     CDS             22539..23648
                     /locus_tag="PLD15_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB62358.1"
                     /translation="MRLRDFKLEVYFEKYEFKAPYLLAQSDCESLSARELLELSGEGP
                     DGLLDTWLGYTQVRGEPSLREKIAGLYESASMEDILVHSGAEEAIFNFMNVFLEKGDH
                     VICQFPVYQSLYEVARSIGCEVSPWELRQTAKGWEFDLSLLERLIRPETRLLVINNPN
                     NPTGYLIDNEELGEIAALADRKGIFVFSDEVYRGLELSEETPKQRAFADICENSLSLG
                     VMSKSYGLAGLRIGWIASHAKDIMESMTRYKHYTTICNSAPSEYLAERALECKEKILA
                     RNAEIIKNNITLAESLLLPTGLIRLNRPQAGPIAFNEYLGGDVDAFCEELLEETGVLL
                     LPGSVYDFPGNYFRMGYGRSGFEEGLQRLKSFLTG"
     gene            23679..24527
                     /locus_tag="PLD15_00280"
     CDS             23679..24527
                     /locus_tag="PLD15_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB62359.1"
                     /translation="MVQKENVLLTVETLKDSFTKTESRIASFVLENPKKVIYMSLTEL
                     SDALKVSEGSIVRFCQKAGFSGFHPFKIAMAMSESPARDEIGGVSDPESLSELKNYVA
                     QRYMDVIRDTSEFISEETLRECVNAVLSAGTVLLVGVGASGNTAQDAFYKFMRIGLNF
                     KYSSDAHLQAMMASQLTEKDVLMAISQSGSTLEIVDIANIANKGGATVICVTGYARSP
                     LAKVSSHVLLTPTRETPFESGAIRSKVAQLYVLELLFMAVFQRMRETGQKSIEKTAEA
                     VAKWIY"
     gene            24788..25801
                     /locus_tag="PLD15_00285"
     CDS             24788..25801
                     /locus_tag="PLD15_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HPB62360.1"
                     /translation="MAERLIEVFTDTDPVENMEKLIEEKTITGYWTIKTSEKLWETKI
                     LVMAENSEKILDSLEQYFSGSEKFKAIIVQVEASLPRVKEEKEPEEERQEEKQPEKGI
                     FKRISREELYNDISEMISNDAVDIAMTILSAIVATIGLVRNSPAIIIGAMVIAPMLGP
                     NVAQSFATTLGDFSLLAKSVRTNLIRIGLGFAFALAFGLLLNLDYNTHEITSRTVADI
                     GDIVLAFSSGTAAALSITSGVSTSLIGVMVAVSLMPPLASAGMLLGKGDIAGFSGAIL
                     LFFVNIVCVNLASIITFRLQGIEPRTWWEANKAGKAVRVTILGWLVILGLLVLSLYLS
                     RLL"
     gene            complement(26649..27305)
                     /locus_tag="PLD15_00290"
     CDS             complement(26649..27305)
                     /locus_tag="PLD15_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPB62361.1"
                     /translation="MCRMLAFKSPTKIESLEIVDILRNMAREGLNNPHGDGFGLAMAG
                     GERLRYNSVRSICEADPPLIESRLGIVHARKASPGYSVNPDHVHPFYGVVKGKNYAFC
                     HNGTVFDFASAPGTIDTQRYFELVLSHMERFSPAEALGRAAGFVADNFRYTSLNAFLT
                     DFESIWILRLNSEKNDLDHGLYLYEREGLKIVASEPVESFSGLPAGSREIANGESLVL
                     "
     gene            complement(27308..27907)
                     /gene="pdxT"
                     /locus_tag="PLD15_00295"
     CDS             complement(27308..27907)
                     /gene="pdxT"
                     /locus_tag="PLD15_00295"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPB62362.1"
                     /translation="MKIGVLGIQGDIQEHLAVIKKLGHEAVWIRDSDALKTVEALIMP
                     GGESTTMTKLLKRFAIWEPLRERINSGMPVFATCAGMILLSKGIENYPAQDTLGVLDV
                     TIERNGYGRQVDSFEADLYVSYFGDVPFRAVFIRAPKIATTGKEVEVMACFGGTPVLV
                     RERNIMAASFHPELTDDVRLQRYFMDMVGNSVENAIPRM"
BASE COUNT         6751 a         7059 c         7699 g         6317 t
ORIGIN      
        1 aatacgggaa acatagagac ttatgtgtat ttgaaggccg aacaggtctt cgagagcgat
       61 ctggaggagg aaaccttctt caaagtcgcg tcgtccccat acccagtact gagagtggtc
      121 gggatctttc acgacaggaa cggcaatgga aggatagacg acggtgagcc gaagatcgag
      181 ggtatagacc tgccggtgga agccagcgag aagatcatcg ttcgtgttat ggtggaggat
      241 aagggcagtg taatctccac cggtttcgtg aacgtcgtcg gggacgatat gatgggaaac
      301 gtcgacgagg acaacatcgc gatgatcgag ataaaggaag gcgagattct aaccttcgag
      361 aagactcagc tgaacgaaac gatacagccc ggcggcactc agcgattcac cttaagggga
      421 aagaaccttt cggattattc tctggacaca gtaaccataa gggaatacat agatttcaac
      481 ggctacaacg tcgaaggcat agcggacctt gactccatcg aggccaacct gccgtacacg
      541 cccggctatt tcgacggtaa ggaatggtcc gggactcgcc cgggaagcga tgaaatcgtg
      601 aagggcttcg aattgacttt ctcgaacgtc ctagccggtc aggagatagt cgtcgtcttc
      661 gatgtcttct ttccgcctac ggcctctccg ggcgtaaggt tcaacgccgc ggagctgctt
      721 tattcgagag cgggcagttt ctgggagggc cggaccaaca ccgtggagtt caatatacca
      781 gtctccacag ttccgttcct cggacccctc ggaaagcctc aggcggcgga aatgaccgaa
      841 gaggacacga cggtgagcac cgtcacggtg tacggtggcg attccgtcag ttttccgcac
      901 acgctcaaga acggtggaaa caggagcggc gtgatggatc tggagatcga gagcgccaat
      961 ttcgatgcgg agggatggaa cttcgtattc aaagactcat cgggtgagcc actggtcgac
     1021 accgacggaa gcgggctggt cgatgcggga ttggtcgggc cgggagagga aagagacgtt
     1081 gttctggagg tctctacccc ctcgcaggcg agcggtgaca acgcggggaa gagcttcagg
     1141 ttcattccgc gtaccgtcat gaacggcgag agcaacagaa ctatcgacat cgttccattc
     1201 atatacgaga gcggcaagtt gagcctcgag aaggccgtga cctcggccac gctccttctg
     1261 ccgggcgaga gcgtacgttt ctcgatcatc gtgaaaaatg aatcctccag cgccagcaaa
     1321 gccgtcgtca taacagatga aatttctacc atgctcgagg agccgttcga tatctccgtc
     1381 tcgaaggagg caacggtcac ttacgacggc ctttccagga ttttgacagt cgaaatcgat
     1441 tcgctcgatc ctttcgagag cgtcgagatc tccttcagct gcaaaacctc gctttccctc
     1501 gaagagggaa cggtagtgaa gaatgtggcg aagctgtcga gcggcgcaat cggcgagact
     1561 tctaacgatg tttcggtcag gatttttcag ggaagactcg aagtggtcaa atcggcttcg
     1621 ccgtcggtag tcgagctcgg ctcggagata acctacaccg tcgaggtttt caacccgtcg
     1681 tcgatagcca ccgtgaccga gctgagaata gaggatcgaa ttccagccgg gaccgtgtac
     1741 gtcgagtcga gcgcgacgat agacggcgag ccgatcgagc cggtcgtgga ggagggtgtg
     1801 cttcacttca ccggtttgaa agagctcgga ccggggaaga gattgacgct cctatatcgc
     1861 ctcagattgg ttgagtacac cggcgactcc cttcaaaaca gagttctcgc cgcaggaacc
     1921 gtgctgtcgg aagagtacag cgcttacgtg accaccgcgc cttcggtcgt agatgtgaag
     1981 attttgaaac ccatgaacct tgccggcgga atatccggga ggctctacgt cgacgagaac
     2041 ggcaacggct attacgacga agacgatacc gctcccctcc cggtcaggct gctcctgcag
     2101 gacggttcct tcgccgtatc cgatagagaa ggcctattcc atttcgacag attgagtccg
     2161 ggaagccacg tgcttaagat agatatagac accactcctt ataaactggc cgctctccag
     2221 gacagccggt cgcttaaaga cggccgttcg tatatgctga cgatactgcc cggtatgtac
     2281 acgatcgtgg atatcccgct ggttcacaga gagaaagcga tcgaaacgat cacattcaaa
     2341 gagccttcga cgctgaaggg gcttgaaggt tcctccgcga tactgtcggc tttctatgaa
     2401 ggctattcgg caggagattc cagcgacggt tatatagttt ttcccgagaa cggccgggat
     2461 ttcaccggca ccgacaggat caccgtcgag gtggccgtcc cgccggagag cctttacagg
     2521 ctttcggtga atggttcaca gatccccgat acacagatcg ggcagaaggc caacgacctg
     2581 gagggaagat ggcttttcgc caggtatttc aacgtgaagc tgacgccagg ggccaacaga
     2641 attcgcgtag agtggcagga tctaacggaa aagggcagcg ccgaggttca ggtgtatctc
     2701 tcgggcgaac ccgttgagat aagagtctca tccgaaccca gggttctgat cgccgacgga
     2761 atcacggagg ccgtcctcac catatcgctg gttgacgaaa ggggaatacc ggcatcggtc
     2821 gggggcacgg tgatagtgga aggactcgat cccgacagcg tgctggagcc gggagaaaac
     2881 tgggatggga agagactgaa actggaaaac ggcgtagcgc gtataaagtt aaagcccttt
     2941 ttcaaggccg gcacggtcga gttcacggca agctacgcgg agctttccag agaagtcagg
     3001 ctcgagtacc gtgtggaatc gaggccgccg atcgtaacgg gattcggccg gctccagtac
     3061 aacctgcact cgaaaagctt cgactacggc ggcggcatct tcggaaggtt caacctggga
     3121 gacgggctgg tgacgctcag gctgggcgaa aaggcttacg gagattacga ggcttatgtg
     3181 acccacggcg acgcctcgac ctcaggaaca cttgcccctt cttccacgtg gtacttcttc
     3241 aggtacgaga tcggcctctt caacctccat ctgggagatt acattttccg gagcgagggc
     3301 ggtcagggct tcgcttcaca gggcacggga ctttcgagcg agtacagcgg cgataagctc
     3361 tcttacaggc tctttctcaa tccctcttac agagagacca ggaaggagga gctcagaggc
     3421 cagggagtcc gggggccgta ctatctgaat gggacgccca tcaaaaacag tgaaaccgta
     3481 ttcctggtca gtcgcgatag ccagggagaa gagctgacca ggaaactcat gaagcgtggg
     3541 acggattaca ccctttaccc gaacggactg ctcatattca ccagacccgt tccttactac
     3601 gatatcgatc tgaacgagat attcatagag gccacctact ccgtggattc cgatctaccg
     3661 ggcgaaatcg acgttatggc caccctgtcg tacagagaaa agggctggaa atactccttc
     3721 acgggtctgg tgaaggggct gctagaaggt tacaggttcg cttcgatcga agtatcggca
     3781 ccgcccatcg cgtcgttcca gccggagttg aacgtctatc tctcttcgaa ggacggggag
     3841 attggattca gggcgcaggc cgcgtcaacc atgaaaaact ccttcgttag cggaaagctc
     3901 gaggtacata tcgacaggca attcctctct ccaaacagct cgagggtatc gtctggctgg
     3961 ggaatcggtc tggaactcgc gcccgtcttt ccaaaggtca ggttgctttc cgggtacaac
     4021 tacgacgaga agaacgaact cggtacgctt tcggccgagc tcctgacgga cgtgaaaacc
     4081 ggccggattt tcaccgagat ttcgctcaag gacactctga tacaccgcca ggccggtttc
     4141 gagaacgaag cgaggctaac tctcaaaccg tctttctccg tagagagagt caaaatcaac
     4201 ggtgaagtgt acgccggttt gaagggtctc tcgccgatcg cgggtctttc gctgcgcgtc
     4261 gatctgggcc tgagcgaatc gctcttcacc gggggcgatc tttccttcga ttatgccggc
     4321 tccgatcggc tcaagctctc actcaccccg ttcatactca agaaactggg agagatctct
     4381 ctggtggcca gggagaagat cgaattcctg cccgatctca ggttcaccac ggtgatcggt
     4441 ttcggctaca cgctggacaa cggtaagttc gatctcgagg cgacgctttc cgataaggtt
     4501 tcccttgggg ccgggtatcg ggcgaccttc tcgccggaaa tagtgaacgt cgatcttttg
     4561 attcagggga ggtacaccgt tgcgggaacg ggcaccgagg atttcctgtc ggcgcacctg
     4621 ggaatagaaa accgggagat cgaacacttg aagttgagac tggacacgga tttgaagatc
     4681 gggctcgatt tcaagctctc cactcttcta atagagagca ccggcgagta tctcggtttc
     4741 gaatcgttga agccggcgta ccgcgttctc ttcgcacagg atttcagact gcagaacagc
     4801 aaattcgaag tccagaccgg actggcctac cgccccgatt ggcgtacccc gttgacggtt
     4861 ctcttcgaag gggcttacta catgaacaga caaaaggaga agatcgactc cgaaggtaga
     4921 ttgacgctcg atgtggccta ctggcttgac aggaccgtta atctctcgct caccggcgag
     4981 atctatctca aaaacgcact cctgtatact gtggggggtt tgcaggtcga aaaacacttc
     5041 ttcgatctct tctctctatt aggcggtgct tacctcgtga gcgacaacgc gggcaacctg
     5101 tacaacaggt tcacggtcga ggccggtttc tctccccttc ccgacacgca gttgtacgcc
     5161 ggctacggct ggggaactct gctggaaagc tttaccggca atttgcagaa gagcggattt
     5221 tacttcggag tccgggtgaa gttcgacgac ggctggttct ttaaaaacag tgacaggggc
     5281 agtctgagcc tgttgttctt cagggacaga gacttcgacg ggcagatcga tcccgacgag
     5341 acgcccgtca gggcacgcgt taaaatcggc gagcaggagt acgagagcga tgagaacgga
     5401 gaagtaaggg tgaatcttcc ggccggtctt tatacggtgg agctcctcga atatccggga
     5461 gatctgattt ctctgctcga cggatctgcg gacttgagtg tgaccgggta cgggaccaga
     5521 cagttcaact ggccgttcat ggaatcaccc gcatttctgg acataagggt ctttgtagat
     5581 tccaactcct cgggaaagct cgacggcgat gaaaaacctc tccagagctt ttcgctgagc
     5641 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacgagcc ccttctcgac
     5761 gcttcgggca ttctcgccat cgggggcaag cccttcagga taggttctcg gacggttctc
     5821 gagggagtgc cttccggcag gagtccggcc agtttaacga tggacagggg cttcgaaaag
     5881 ctctattcga gaatcacgac aatcgatacg gttgaagtgg ccgaaacggg ggacactgtt
     5941 ctcgaaatag ggttcgccga gaagagctcg ctcatgatat ccttcctcga tgagaagggc
     6001 gactatttct acgatgtcgt aacggtgaag atcgatggca tcgagtttca ggcctttggt
     6061 ataatcgagc tggtgggact cgtcttcgga gaccacctgg tggaagtaac cggacttccg
     6121 aaggggttca agtgcgaccg gtacctctac gaaatctggc tggagcccgg cgggagcggt
     6181 gaaatagaag tggtgatagg gaaggagtga tactatggac tttttcaggg ctctcaacga
     6241 gattgctttc tatctctcct tcgtactatg ggccgggggt ggtgtcacgg tatcggtttt
     6301 ccttattccg ttgcgcgacc cgaacacacc tccgcaggtg cagggttttc tgtactcgct
     6361 ctgggagaac ctcaggatag tggcgatggc cgtctcgatc atcatgctcg gcacgagcat
     6421 gacgaggctt atcatcgatc cggtcgtcaa cgttttctgg acggtgatat gcttcgccac
     6481 cttttcgctg accgttttca gctggtacat ggccgacagg cttataagga tgggggccag
     6541 cgatccacgc tcgatgctgg aggtagaaaa gagaaacttc ggcctctatc tgttttacgc
     6601 agacgcctgc atagtcctga cggtaatcgc ggcggccctt atgtaaaaag agacattagc
     6661 tctggtttac cggcagagag ttcgacagaa cacgagcacg gacacaaact ccagaggagg
     6721 cccgcccgaa tggcaggaag agaatactcc ttcaaacaga agatcatcga aacgcttcac
     6781 gacattacgg tcagagacat gtcggtcggt atagacggta tcgcttatgt aagcatatcc
     6841 gtgccatcgc tcgaagtctc cggccagagt tccatcgacg gctacttcaa agccggctac
     6901 tatccctctt cgaagggaag gcccgcgcat ctgggcataa gcctgaaagt agatgacaag
     6961 cggcacgagg gtatgacgct ctctctcgaa gcgaccgctg tcttgcagaa gtggtatgaa
     7021 aaggcgtacg cggatatcaa gaggagattc aaatccatcg tggatcgagt tgttgacgat
     7081 gagcttccgg taatcttctc gctcgcaggc gacgactttc cgaaattcac tccctctctg
     7141 cagatagaag atgaggactt gaaacatgaa agctacaaag tgctaaaaca cgccatcgtc
     7201 aggtttttca agctgcacgg cgccgaaagg tacgcgaagg ttgcgcgtgc ggacgattac
     7261 ctcgtagaat cgatcccgcc cccgcccgta agcgacgatc gactgccgcc gacggccttc
     7321 gacatagaac acgaaagaga tctctatgac atttacggta ccggaccggg ttcggtgttt
     7381 tctttcaaaa tgaaactgtc ggatatactc aatcccgggc ttaaacagct gctagagcgc
     7441 gaagaatatt agaggtgaaa ggtgcaaaag tgatcgttat cccgcagtgc tccgattaag
     7501 aaccgttgta ggttgtgagt tatgggtaag ggcggnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnaaaca gaggtaagag ggggagaggt cagagggggt tccccaagga
     7681 cggttctttc cgctctttcg aagaacggct cactccccca gaagtctgtc gagctcttcg
     7741 agttctcttt cgaggttttc tttctcttcg agtaactcaa gtatcttctc ttccgatgat
     7801 tctatggtgg cctgaaactc ctgaagtttc tcgtagttag tgacgtacag tcccattttc
     7861 agctgggctc cctctatctt tctcgatagt tcttcttctt cattatggag aagatcgtat
     7921 ctttcgcaaa gcgatttccg cctgttggtg agctttcttt tctcctggaa ggagagcctg
     7981 gaggcttcgt tatcgttgct ttctctggtc ggcctgttac ggttcaggta ttcctggacc
     8041 gagcgcacgg atctgaattt caacctgtct atcatcacga agtgatcgca gatgttctgc
     8101 acgaactccc tgtcgtgcga gaccagtatt atacagcctt cgtactccct cagaacatcc
     8161 tccagactct ctatggacca gatgtcgagg ttgttcgtcg gctcgtccat caccagaacg
     8221 ttcggctttg agagaatcaa tttcgccagc gccagtttcg tcttctctcc gccgctcagt
     8281 gaggagatgg gcttgaagac gtcttcgccc gggaagccga accgtcctat atatttgcgc
     8341 acctcgtaat cgggctggcc cggcatcagc tgccatatct ccgtcaggac atcgttgttg
     8401 aggttcagat cttcggtgag ctgagagagg tacccccagc gaacgttgtg accccactcc
     8461 acctccccgg aagttttctc cagatcctgg gttatcatct tcaaaagcgt ggtcttgccg
     8521 ctaccgttcg gacccagaag gcctaatttt tcgcgtctat ggacttccag accggcgttt
     8581 ctgaacaact ccttgccgtc gaatccgaag gaaagttctt cgactttgag gacaatgtaa
     8641 ccggtcctgt ctggctgggg aagcctgaac ctgggagccc ggacctcctc gtcgggtaga
     8701 tctacagatt caagctgttc tttgagcctg tcgcgctgtt tctctttgtt tatggcctgc
     8761 tttatgaact tctcctgtcc ccatagcctg taacgtttgg ccacggcgtc gagcctttcg
     8821 atctccttct ccagattctc tctggctctc gttccacttt tcatgagtat ctcacgatcg
     8881 aaaaggtact tgtcgtaatc gccggggaag tcccacatcg aaccgccgtt cagctcccag
     8941 aacctgttac cgaccttcct tatcaggtat ctgtcgtggg ttacgaccac cagtgcgccc
     9001 tggtagctct tcaggaatcc gaccagccac tcggtcgact caagatccag gtggttggta
     9061 ggctcgtcca gaagcagaag gtcgtaatcg acgaggaaaa gctttcccag agagattctg
     9121 gtcagctcgc ctccgctgaa ggtattcaac tttctgttcc agtcctcctc tgagaattcc
     9181 agacctacca gcgtgctcct gacccgtctt tcgaaggaat agtactcctg ggtgtctggc
     9241 accaaaagag atttatcgct catatagtag tcgatgagcg ttatttcact gtcgggaatc
     9301 ctgttctgta tctggtaccc gattttcacc gaagtggagt ggaaaatctc cccctcgctt
     9361 ggcttcagct ctccggctat tatcttcatg agggtcgatt taccgcaacc gttttttcct
     9421 atgagtatga tcttgtcttt tttgtctatg gaagtcgaga catcgtcgaa gagaaaatct
     9481 tgaccgtaat cgtgtccgac tttactcaat gatacaagca tctttttttc ctttcgtcca
     9541 atcttctcag tcaatatgat actatacaac ggggtttttt tatcgaagtc ctttacggga
     9601 tataatgttg gttgggtatg tgatatcttt gagtatgcaa ttcgattggt ggtgtttacg
     9661 attgaaactc ggagtttcac tgttgatttt cgttactacg tttctggtga tgctttatat
     9721 atggggcttt tattttttca agagccccgg ccaggttttt gaagagtacg aacgcgcgct
     9781 ggtggagtat gcccaccaga tgcgaataca ggaagaggcc ggcttgaagg ccgggctggg
     9841 cgctcgagag ctgtcaaatc ttagagaagc tgccatagtc gatctcgagg gagacatgaa
     9901 aagaattgtg gcagacttcg agaaggactg gtcggccatg cggaggatct acctggaata
     9961 cgccagaaga tcgggtagct ggttcaccga agggttaacc ggcgtcatcg tcacacagga
    10021 caatcccgat tatcacactc tgctggccct ctataacctg atagacgatc ccgacagcta
    10081 ttgccttttg ttcataccgg aaacgaacgg ttcccgtgtt ttcaacgagt tttccggggc
    10141 gagactttac tatctccaga agactttgtg gggctacagg atcgagtgga gcaaatctct
    10201 catcgatctc ctgttgggta aagatattac ttcaatagcc gtttaatata aggaacaccg
    10261 ggccggagga tagagatgtt tctcaaaaga ctcgtggctg ttctactcat actcatcgta
    10321 accgctacac tggtggccag tccagaaatg ttggcccggc agacgttcgc ggccattctc
    10381 ggcggatttg ccggcagcgt ctcgggggcc gtcatcggta cggtactggg cgggctgatc
    10441 aacccgaccg gctggggtga tctcgttggt ctgatactcg gctttattgt tggcgcaccg
    10501 gtcggcagct ctctaactac catctcctgg tcggttgacc gactggaagg aagtgtgctg
    10561 ctctcaatag ccggaagcat aggcggtgtg gcctttcctt acgcccttag cggattgatc
    10621 gccaacgtgt tccccgcgaa cgatttcaat ccctccggta tcgtcctgat gatttcctta
    10681 cctctgtatt cggcgtttgg agcggtgctt ggctataatt atccccgcct tatttccggc
    10741 gatctggttc tagaggatct atcgacaacg gtaaaatgat tcgccgtcgg gagaacttta
    10801 ccggattttg gcgtgtttag aggctaaatt agggggcttt ctgtgataac attttcctga
    10861 taaggctaaa acaaacctgc agacccaatc cattgaaccc aggaggaaat gatgttctgg
    10921 aaagagaaac gagtcgagaa gaaactggaa aaagagatcg gaagaaagga aagggagctg
    10981 gccggcagca ccgatcctct gcagcgtggc tctctgctgg atgaactggc gttgttgaag
    11041 gaagagaagg gcgatctgtg cggagcgatc tcggcgttac tggataaatt caaagaggac
    11101 cggaaggtcg aaactctgaa aaagctgcga ccgatcctcg agaagcacag tgaatttgat
    11161 ctccggaaac tcgaagagag acttttcgag tctagcgaaa atctcgatcg cacaacggaa
    11221 aacgatctat tgaaggagat ttacagcatc gacggaaagc tggcccttca actttcgttg
    11281 aggtttttcg agatttccag agaatacgcc tgcggtttga gcgcgataga tatcatggaa
    11341 agaatactat cggaaaacgg cagggaacac gatgtctcta ttgtggagac cgtggaggag
    11401 taccacaggc tcgtgaacga actggtcgag atttcgccgg acagaggcga gcaaatactg
    11461 gccagggctg ccggcttttc caaagagaaa ctgggaaatc tcgagccctt tctggacttt
    11521 tcgctacgcc gtctcgggga tgagtcgtct cttgaggtca ggtccggcat agtggacagc
    11581 gtaatagaga aattcgataa cctagccacc gtttttgaga agagggaagg agaattgctt
    11641 aaccggctgg aggttctcct ttcaccagag atggagtgca agagtgtcga tttgctactc
    11701 gagcacatga tagagactgc ccgctcgata aacgactcca gagaagagct ttaccttgac
    11761 aggaagctga agcgggccga ttcccaatcg ctgaagagcg aagccatggc gatcgagaga
    11821 gctttaaaga aaggtgttcc cgacatttct cagtggaaga ggctggcaaa actgtaccgg
    11881 tatgtaattg atctctctca agacctcgaa gagaagatcc tgtacatccg aaagctcggc
    11941 gtccttctga gggacaagct cgaggattac gaaggctctt tgagggtttt cgaagaggga
    12001 atgaagttga aggaaagcga tataactctg tggctggtta aagggatgac actggagaag
    12061 atggcgcaga accatccggg ttcggaagca acgtgcgata tatggaaagc cgcttacgac
    12121 cattacttga agatagccga aaggttcgac gaacaggaga taaagctctc ggcgatcgaa
    12181 aagtgcgcca tcatacaatc cgaaaaactc agtgtctagc caggtacgat gcgtaggtga
    12241 gagggtagat gtgtgaggtg agaagagcta taagatcttg actttctaac atctcgcctg
    12301 tcacctctta cctctgctct agagacagat acgcgacaaa cccttgggtg aatgatgaac
    12361 ataaaaggag agatgatagc catggttgag aagctggacg gaagaaaagt gaaatccctc
    12421 atagaacttc tatcgggctt tcacagagtt cgaggaagca aggattacca cgattcaatg
    12481 atggccgcga tcgattacct gaaaagatcg ggcatgccgt ccagaagatt caaagtcttc
    12541 tcttatccgg ccgacggaaa gacagttaat ggcaacgtag ccaccactct ggcctgggaa
    12601 ccgatctatg gagagatgtg gctggaaagg ccggagaggt ttttcataac atccacctcc
    12661 atatcgaagg tcgcactggt ctccggcagc gccggaagcg aggggtggaa ggaactgagt
    12721 ctggtccagt tcgaaggaaa gaaggactat tccaacaagg ccgtcctcgc cagcgacgat
    12781 ccggtgaaag tcttccagca ggccgtcgtc gaggggggag cgagttgcct catattcaat
    12841 tacatgagga aatctttcga agagatcggc aggagtctgg aaaagcttcc ggagatgacc
    12901 aactatcttt ccatccctta cgacagggag gctgccgaca aaaaggccgt tgccttctcc
    12961 atcaccaaag agaaatatga tcttctggcc ggtgccgttg ccagaggcgg cgcggtcgtg
    13021 ggattccgat cggaagtcaa gctcactgaa ggcgagctcg aagtgcttca agtagatgcc
    13081 actggtgtgg aaaacggtcc cggcgtgatc ataacggcgc acctgtgcca cccatcgccg
    13141 ggtgccgacg ataacgccag cggagcggcc ctggcagtcg agctggcacg gatactccac
    13201 caggccgagt tccccttccc agtcaagatc gccctggtac ccgagtatct gggaagcgtt
    13261 ccatacgcgc ttcaactcaa gagagagaag aagctgccgc tcttcacggt taatctggat
    13321 atggtcggtg ccgatcagga taagacgggc tccaccttca ttctttccaa ggttccaccg
    13381 tacctaccgc agaagtgggg acggattctg gagtatttca taactgagct gatgccaaga
    13441 aacgggggat atcccttgaa acgcttcggc gagataccct ttatggccgg ttccgaccac
    13501 tgtgttttca ccaccctcgg cataccctca cccttcctgg gacacctgcc agacaggtat
    13561 taccattcgg acttcgacac accttccatg atggatcgcg tggaactgga gtgggttggg
    13621 agatcggtcc tgaagacgct cgaacatatc atcacgcccg acccgctggc agcggcagcc
    13681 gcgaagggaa agatgatagg agagctccac gaaatactct cgagaatagc cggtagagag
    13741 ggggcgggcg atcttctcga tctgctcgta tccaattacg agggcgatgt tctcaggggt
    13801 aatttcgcgg aggcctgcgg cctttcctct tccagaccac tcgaaccgac gtttgaaagc
    13861 tctctggggc tggagtggat gaagatcgtt ccgcaggaat tgaagaacga agtgggaccg
    13921 gaacttctca gcatggccga tttcatggct ggcggttcgc tggtcatcgg aagcagggaa
    13981 gccgtggaac tcctcaccag cattcattac ggggtgccca tagagaaggt gagaacactg
    14041 acgggctgga tgatagaaag atcgcttttg aggtcgtctt agccatgttt ctcaggtttt
    14101 tgtggctctc gcttctgctg accgcggtga ccgttgcggc aggcagtgtc gggttgcagg
    14161 tcgatacggg gatctccatg ccctggaacg gatacctgaa aaacatagag accggagaga
    14221 agatcgaaat aataaaacgg gccaacttcc gcgctttttt ccagacgacc atcggttaca
    14281 ccttttttga agaacattcg atagaagcga tggtttcgcc cttctcgatc gaaagccgtc
    14341 tggaacagga cggcgatgaa gaaccggccg aatacacttc cagatacgtt caggatacgt
    14401 accggctgag gtacaggtat ctactcccac ccctggacag gttctacctc cagataggac
    14461 tgcacgccac cctgcacatg aaggaactgg ttatcggcgg ttcaagggaa tacactaacg
    14521 aatggactga cttctctttc ggaccttcgg ctcttatcag tctttctatg aacgagatag
    14581 tctccctggt gatggaagtc gaagccggca tagggcgcga caacggcttt ctggatatct
    14641 tcaccgggct gaacctcagc ggtcccttct attttgtgcg ggccgggata agaaatcttt
    14701 cgacgaacgg cttttccggc ggcgaggctc tcagcacttc cttctggaat ttcggcgcac
    14761 agttcagggc cgttttctga ttatcaatct tcgaatctca gcggttccag agaagagatt
    14821 ctgaccagtc ccagcaccgg aacgccgaac tcttccagca gattccgacc ctcttcgaaa
    14881 gtcttttcta tgacggccgc gaatccgcaa agcttcgctc cggtcttctg tacaagcctt
    14941 cccagagccg ctatagtttg gccgctggcg agaaagtcgt ccacgatcag aaccctgtcg
    15001 gactctccga ggaactcgag cgatacgtta agctctacga ttcccccttt ggtgtgcgag
    15061 ggcgcggttt cggtgatgta ccgtttcata gttatgggct tcctcttcct ggcaaaaagc
    15121 agcgggactc ccatgcactc ggcggtcgcc agcgccgggg cgatgccgga gctttccacg
    15181 gtgagcacct tggttatctc tgcgtctttg aaggtatcac agatcaaatt gccggccgtt
    15241 ttgatgagcc ggggatcgat ctgatggttc agaaaggaat caactttcag tattccccct
    15301 ccaagtaccg atcccttttt gattatggct tcctgaatac tcctgacagc ttcctctctt
    15361 gaaacctcga tgatttccat gcctcgacct ccggaaaata tatactgata tgatcgactt
    15421 ccaatatgtc tatcctttga agtattcgtc tcttcttgga acgagtttga tggtcgggag
    15481 cgagataagg gtgaccgcca tcttgacgat gatgttggat aaagtgatcg atagaacggt
    15541 aattagcggc aacaccccgg caaaggcgat gaacgagaaa ataaaactgt cgattaccag
    15601 cgcgatgcca ttcgaaaaga ggacggcctt aatatcgccg aacctccggt acaccctgtc
    15661 gaaaacctcc gtgtctatga gctcggagaa gacttctgct atcatgctcg cgatagttat
    15721 tctccagaaa ggcatcaaga tgctttcata ggcgccttgg agattccagg tggggtcggc
    15781 cggcatttta ccgatcagcc agaagagaac ggccatgagc aggttgagaa tcccggccgt
    15841 tatcacgacc tgtctcgatc tctcttttcc ccacgtcttg tgcacgaaat ccctgagggt
    15901 gaaggtgaag ggatagataa cggtccctcc gtcgatggcc agatgaagga agggcagcgt
    15961 ggtgatcttt atcgagagaa tatccgcgaa tatctgaaaa gatatgtaaa gggagacgct
    16021 caatatccct atgtctatca tacttcttcg atttttcatt ttcccggccg actcctgctc
    16081 acgtcaatcg tatccaacga actggaactc tcaagaggaa ttatacatac attcgcgaaa
    16141 gatagagttt atgtatcttg aagttcgaat aaagctgttc attcacgaag caccaaatgg
    16201 tatcattttc cttgaaggga tgaaaagcga cgggagagcc tatggttgac gatcacactc
    16261 cggagataag gttcgataca gatctagaag gtatcctcac ttacgcggta ataggaacac
    16321 gctactgcga caggtggatc ttcgtgaggc ataagctgcg ccggacctgg gagatacccg
    16381 ggggtcatat cgaagagggc gagactcccc tcgaggccgc gcgaagagag ttgcaggagg
    16441 agacgggagc ccggtttttc gagctctacg agatctgtac atactcggtt tcgaaagatg
    16501 gagcaacaac ctccggaaag ctcttttacg ctgaggttac cggacttgaa gggcttcaac
    16561 ctcagagcga gatcggggag ataacgctcc gggtcggtct tccggcccga ctgacttatc
    16621 cggaaattca gccgtggctt ttcaaaaaaa tcgtgaaatg ggagagaaac tttgagcgaa
    16681 gctgatgccg tttcaaaaag ccgttccgga cccgtgaccg tgatttcgct ccgtaaggat
    16741 ttcgagaacc ttggaatcaa aaagggtatg agactcctgg ttcactcctc gctcagcaag
    16801 ctgggctggg tttgcggcgg tcccgtggct gttataagag ctatcgaaga agtcatcgga
    16861 gtggatggaa ctctcgttat gcctgcacat tcgggcgacc ttagcgaacc ttcgagctgg
    16921 cagaacccgc cggtgccaga atcgtggcac gggacaattc gaaagaccat gccccctttc
    16981 gaccgctcgg cgacgcctac cagagggatg ggcaaaatac ccgagacttt cagaaagatg
    17041 aaggccgtcg tcaggagtaa tcacccgcag gtctcgttcg tcgccagagg gaaggacgcc
    17101 ctgtacataa cggacgatca ccagctggat ttcggcatgg gcgaagggtc gccgctggcc
    17161 aggatgtacg agctcagggc cttcatcctc ctgatcgggg tctcccactc taacaacagt
    17221 tctttgcacc tggccgaata cagggccgag tacccttcaa aggaagtctg taaagacggg
    17281 gctccggttg tggaaaaggg ccagagggtc tggaaagagt tctccgacat ctcgctcagc
    17341 accgacgact tcgaggatat aggcaaggcc ttcgagagcg aatccccctc cctggtgagc
    17401 cgtggaaggg taggctacag cgacggcctt ctgattccgc aaagagaact cgtggatttc
    17461 gccactgtat ggatgagcga gaatagaaag tgagcggccg gtaagggacg attttcggta
    17521 accgcctgtt tcctgcaggc ttcccgtaat agtttataat attcctgaga agtcgaaagg
    17581 aggaatagtc atgatgaagc tagtcgttgt aagacacgga gaaagcacct ggaacaagga
    17641 gaacaggttc accggctgga ccgatgtcga tctttccgag aagggaaagg aagaggcgaa
    17701 aaaggccggc gaaatcttga aggccgaagg ctttgaattc gatctggcct acacatccgt
    17761 tttgaagagg gctataagaa ctctctggta cgttctggac gagatggatc tgatgtggat
    17821 accggtgata agggactgga ggctcaacga gaggcattac ggcgccttgc agggcctgaa
    17881 caaggccgaa acagccgcca gacacgggga agaacaggtg aagatctgga ggagaagcta
    17941 cgacatacag ccgcccgcgc tcgaagagag cgacgagaga ttccccggcc gcgacccgag
    18001 atacggaaat ctcacggccg accagcttcc aaaaaccgag tgcctgaagg acaccgttgc
    18061 gaggtttctg ccgctgtgga aaaatgaaat ctcaagcgac ataaaatccg gaaagaaggt
    18121 tctggtagtg gcccacggca acagcctcag ggctctggtc aagtacctgg acaacattcc
    18181 cgacgacgag atagtcggtc tgaacatacc tacgggaatc cctctcgttt acgaactcga
    18241 cgacgatctg aaacccataa agcattatta cctcggtgac cccgaagaga tcgccaaagc
    18301 ccagcaggcc gtggcgaatc agggcaaggc gaaatagccg caagtggaaa tatatgagaa
    18361 atcgggaacg agcgaagggt tcccgatttt tctatgggta gtacacaggc gaggtcccgg
    18421 tcgctgaaac cacccctatg gtaataaaat agaccggagg tgagagatgt ccatattcac
    18481 ttttcttatg gatctggcga ggaaggagtt caagatcgat tctctttccg gtcgcgacag
    18541 gctgggcttc gaccgaccgg caggtctgta tatacatatc cccttctgca gtgagagatg
    18601 cgatttctgt ccctacaaca aagtgaaata cgaccgggaa gcggcggccg gttacccgca
    18661 ggctctggag agggaacttg aactccaggg agtgaagggc ttcagctctc tatatatcgg
    18721 cggtggtacg cctacgctgg atatggatct cctggaagac gtaattgacc gtttctcgaa
    18781 ttcaatcgat ggagagcttg cgctggaagt ccatccggcc gatgcaacag ttgaaaaact
    18841 cgagagaata aaaggaacgg gaatcgattt cgtaagtttg ggaatacagt ccttcgacga
    18901 cagcaccctg cagaagatgg gcaggaaaag acagagtgcg gccctttcca gggaagcgat
    18961 cgagagagtc gttctggctg gcttcgactt tgtagacgtc gacctgatct tcgacttcga
    19021 actcgaaagc gagagaactg cacgcgacct ggagatcgcc atggattacg ggccacacca
    19081 gatctccgtc tatcccatga tgaggttcac cggcacggcg ctggccggta cgaaaaacga
    19141 tcccgatagg gagttgcaga ttctcgagat actcgaagag agagcccgga aaagaggtta
    19201 ctctcgggac acactctgga ccttcaaaaa acatggcgaa tcgaagagat acagctccgt
    19261 ctcgcgtgag ttcttcctcg gactcggcac cagcgcctct tctttcaacg ggggacaatt
    19321 cacaaccaac actttctccc tggacgaata ctaccggtac ctcgacggag accgcattcc
    19381 ggtaaggagg gcgatagaga tgaatacttt tcaggcttct ctctactact ctttctgggc
    19441 cctgtacggc ggttcactcg atcaggagag actgagatca ctctttggag agccggacat
    19501 caggctgtcg gggctgctgg agctggccag aaagacggga tatctacgac aggagcgcga
    19561 aaaactggtt ccaacaaaaa aaggctacag ggagctccat atgatagaag agtggctcac
    19621 ctacaacttc atcgatgtga tctggacgga gttgagggaa gaagcggcaa atagagctca
    19681 cccgggatct gtctggtaga aggtgtcggg atcctcgaac gagaagcggc cggcctttcc
    19741 cgaggccaca tctctgagaa aagtgtgcga acccctggta gtatcgtact ggccctgtct
    19801 ggcaagaaag cccctcttcc tgcagaactt atcgattaaa tcctcggagt tttcgaaaga
    19861 cgctcccgta tattcgaaca gactctcttt tccgtacctg cccacgatga gatcgagcac
    19921 tctctcgaaa gcgtccatcg gatcgaactg ctcgtaaggc agagagccca cggccagcaa
    19981 tttggtagtt atgtgtggcg agtgaaggtt ggtgtagagt attccgggtg tgtcgagaac
    20041 ggcgatctgc tgggatacgt tgatccactg aacaccgcgg gtgattcccg gacggttgcc
    20101 ggccgcgaga gatttcttgc ccttcagcct gttgatgaac gtggactttc caacgttcgg
    20161 catcccaacc accatgaacc tcttttcgaa gaacttgctc ttgaccaggg gcaccacttc
    20221 cctgattatg aactgcctcg cgtcgctgtt catgaggctc gcctctatga cgtccgtgcc
    20281 ctgcgaggag aagtgctgcc tccacagctg tgtgatctgc ggatcggcga ggtcgctctt
    20341 gttgagaacg acgatcctct gcttgttgtg aaagagcttc tcggcttcgt aagccctgct
    20401 ggtcatggga atcctcgcgt ccagcagctc gaccacggcg tcgaccgact tgatattttc
    20461 ctggatctgt ctcttggctt tctgaatgtg accgggataa tacatgactc tacctccggt
    20521 ctggattata gcacgggtac aattctccgg tcctctcgct tctgttgcta taatagaatc
    20581 gaagcaaaga tcaaaggagg tagtactgtg aagacaaaca aagacacggt ggtgaagata
    20641 tccgtttcgg gcgaggtggc gcatcccctc atgagaagcc ctttcaggct ggagatcgac
    20701 ggcacgccca ggcttttccc cggcaccgga ggcattacct acaatttcgc tctgggcgac
    20761 agcgccttca aaatgaccgg cgatcacgtt gagccggacg tttcaacgaa gaatcccgac
    20821 agcgacaaaa acacggccta cgtggcctac tcgtgcatag gcaattcggc tctcgttatc
    20881 agcggcgacg ccaaaggcga gcgcggagtg gtgacgggaa agcacggagg cataaaccat
    20941 gttttgatcc acttcaagga agaggttaaa gagaagatgg ccataggcga caggatacag
    21001 gtcaccggtt acggccaggg cctcgttctg gaagactacc ccggaataag ggtctataac
    21061 atagatccct atcttctcga gagagtaccg gtcgtagaat ccgatggaaa gatacacttt
    21121 ccagtcagag ccatagttcc gggatacttg atgggatctg gctccggctc cgggaacccc
    21181 gccggagggg attacgatat aatcaccaac gaccgttccc tggtaaaacg cgtgggcctc
    21241 gacaaattga ggatcgggga cttcgtcgct ctggagaacc acaacgactc cttcggactg
    21301 ggcggctata tggaaggctc ggtaaccata ggcgtcgtgg ttcacggcga ctgtatcata
    21361 acaggtcacg gacctggagt gactgtcata atggccgacg gcgaggggat aatcgtgccg
    21421 gaggtatcgg aaaagtcgaa catcgttgac ttcatctgag aatcgggtga tgtcgcaaat
    21481 cgaaagatct ttgtccccgg aggggtagta tgagagatcc tggctttggc gtatgggttg
    21541 cgagatcgac gctcctgaat cccgcaggcg tagaaaagat gatctcgaaa ctgcacgaga
    21601 tctcggccgg ccgcgcttac gtgcaggtcg tcggcagggg cgattcgcag tacagctccc
    21661 gtattctccc ccgggccgaa gagctggacg gccctttcga cggcctctcg gagacgatcg
    21721 aaatggcccg cggcagcggc ctgaaaatct cggcgtggat gaacgtgaat ctggtttgga
    21781 agttcggcga caggccctca tcgaaggatc acgtggtcaa ctccaacccg gaatggataa
    21841 cctgcgacga gcggggcctc tcgatgctgg aatactcctc ttgcgatata gaagaggtct
    21901 tcgggccctt catcgacccg ggtatcccgc aggtcaggcg tttcacagcc gagatcgccg
    21961 atgagctggc ctcttacgat gttgaagagg tacacctgga cttcatcaga taccctttca
    22021 ggaccttcgg ttacacacag gcggcccttc acgaatacag gagatggctg cgggccgtaa
    22081 accttaaaga cacggaagag aacttcgaca gattcagaat agaatcgacg ggcgagcagg
    22141 tacggctcat acgcgagaaa gtgagatcga ggggcaaaaa actctcctgc gccgtcttcg
    22201 acgactacag cgaaagggcg gttaaagaga ggcttcagcc ctggctcgga tggctggagg
    22261 atggactgat agattcggcc gtcgttatgg cctacggaag agagccggac ggggtgatct
    22321 cccgcgtcga aaatatttgc aacatacatg gatcgttgaa gggtataaga gtgggactgg
    22381 gagccttcaa ctacgccggg agatgggacg atttcatcgg tctcgtgaaa agggtgagga
    22441 agatggatcc cgacgagata accttctttg cgttgagctc gatcgacgaa gagctcgccg
    22501 ggaagttgaa agctctcgac tgacgggagg ttgtaacggt gagattgaga gatttcaagc
    22561 tcgaagtcta tttcgaaaag tacgagttca aggcgccgta tcttctggcc caatccgact
    22621 gcgaatctct ctcggccaga gagctgctgg aactctcagg cgaggggccc gacggcctcc
    22681 tggatacctg gctgggatac acgcaggtca ggggagaacc gtcgctcagg gagaagatag
    22741 ccggccttta cgagagcgca tcgatggaag acatacttgt ccactccggg gccgaagagg
    22801 cgatcttcaa cttcatgaac gtcttcctcg aaaaggggga tcacgtgatc tgccagttcc
    22861 ccgtttacca gtctctgtac gaggtggccc gttcaatcgg ttgcgaggtg agtccctggg
    22921 agttgcgaca gactgcaaag ggctgggagt tcgatctgag ccttctggag agattgatca
    22981 gacccgagac gcgcctgctg gtgataaaca accccaacaa cccgacggga taccttatag
    23041 acaacgaaga actgggagag atagccgctc tcgccgacag gaaaggcata ttcgttttct
    23101 ctgacgaagt ctaccgcgga ctagagcttt ccgaagagac ccccaaacag agggccttcg
    23161 cggatatctg cgaaaactcc ctctcgctcg gagtgatgtc taaatcctac ggcctggccg
    23221 gcttgaggat cggctggata gcttctcacg caaaagatat tatggaaagc atgaccaggt
    23281 acaagcacta cactaccatc tgcaacagcg cgccttctga gtatctcgca gaaagggctc
    23341 tggagtgcaa agaaaagatc ctggccagaa acgcggagat cataaaaaac aacataaccc
    23401 tggccgaaag cctgcttctg cccaccgggc tcataaggct gaacagaccc caggccggac
    23461 cgatagcctt caacgagtat ctcggaggcg acgtcgacgc cttctgcgaa gaactgctcg
    23521 aggagacagg cgtccttctg ctcccgggaa gcgtttacga tttcccgggt aattacttca
    23581 gaatggggta cggtcggagc ggtttcgaag aagggctgca gagattgaag agttttctga
    23641 ccggttgaat ggggttatca tcaattcgag gtgatgttat ggttcaaaag gaaaatgttt
    23701 tgcttacggt cgagacgctg aaggattcat tcaccaaaac cgaatccagg atagcctctt
    23761 tcgttctgga aaatcccaag aaggtcatat acatgtcgct caccgaactc tccgacgccc
    23821 tcaaggtcag cgaagggtcg atcgtcagat tctgccaaaa ggcgggattc tcgggtttcc
    23881 atcccttcaa gatcgccatg gccatgtcgg aaagccccgc gagagacgag atcgggggag
    23941 tctccgatcc ggaaagcctg agtgaattga aaaactatgt agctcagaga tatatggacg
    24001 tgatacgcga cactagcgaa ttcatctccg aggagaccct cagggagtgc gtcaacgccg
    24061 tgctctcggc cggaaccgtg ctgctggtgg gtgtcggcgc gtcgggaaac accgcgcagg
    24121 atgccttcta caaattcatg aggataggtc tcaatttcaa atactcttcc gacgcacacc
    24181 ttcaggcgat gatggcctcg cagcttaccg aaaaggatgt tctcatggcg atctctcaga
    24241 gcggaagcac gctggagata gtggatatag ccaacatagc gaataaaggc ggtgccaccg
    24301 tgatatgcgt caccgggtac gcccgctccc cgctcgccaa agtctccagt catgtcctat
    24361 tgacccccac cagggagact cccttcgaaa gcggcgcgat cagatcgaaa gtagcccagc
    24421 tttacgttct ggagttgctc ttcatggcgg tttttcagag gatgcgcgaa acaggccaga
    24481 aaagtatcga aaagacggcc gaggccgtcg ccaaatggat atactaattt accgggcggt
    24541 catggctgaa ggaattgact ttaacatatt tccgtgatat aatataattt gaagacgctt
    24601 aaaaggcgtc ttttcttttt ctccgctttt tccgcctacc gcacaaacgg tgtcgcgact
    24661 gccgacgtaa gatacaacgc cgtatctttt cacgatcaga aagctgtcat caccggccaa
    24721 caggtgtagt atattctaaa taagcgaacc gattatgccc ccgctttcta tacaggatgg
    24781 tgatcagatg gccgaaagat tgatcgaagt attcaccgat accgatccgg tcgaaaacat
    24841 ggaaaagctg atcgaggaga aaactatcac cggatactgg acgatcaaaa cttccgaaaa
    24901 actctgggag acaaagatac tggttatggc cgaaaacagc gagaagatcc tggattctct
    24961 cgagcagtat ttttccggca gcgagaagtt caaagcgatc atcgttcaag tggaggcttc
    25021 gctgcccaga gttaaagaag aaaaagaacc ggaggaggaa cggcaagaag agaaacagcc
    25081 cgagaaaggc atcttcaaac gcataagcag agaagagctg tacaacgata tctcggaaat
    25141 gatctcgaac gacgcggtcg acatcgcaat gacaattcta tctgccatcg ttgcgacgat
    25201 cggccttgtc agaaacagcc cggcgataat aatcggggcg atggtcatcg ctcccatgct
    25261 aggtccaaat gttgcccagt ctttcgcaac gactctcgga gacttttccc tgctcgcgaa
    25321 gtccgtgagg acaaacctca ttcgaatagg actgggtttc gctttcgccc ttgcctttgg
    25381 actcttgctg aatctcgatt acaacaccca cgagataacg agcagaacgg tggccgacat
    25441 cggcgacata gtactcgcct tttcctcggg gacggccgcg gcattatcga ttacctccgg
    25501 agtatctact tcgcttatcg gagtcatggt ggcggtctct ctcatgcctc cgctcgcctc
    25561 tgcaggaatg cttcttggga agggcgatat cgcagggttc tccggggcga tactcctgtt
    25621 ttttgtgaac atagtctgtg tgaacctggc atcaataatc acgttcaggc ttcagggaat
    25681 cgagcctcgc acttggtggg aggcgaacaa ggcgggcaaa gccgtcagag tgacgatcct
    25741 gggctggctg gtaattctgg ggctgctcgt tctttcgctt tatttgagca gattactgtg
    25801 agatgcgaaa ggatgtggaa gcataacacg ttttgccgtt cgcaaagttc ttaaatcaga
    25861 cccaggctcg acgggaaata gaaacggaat gaaaaacttt cttctttccc gttgtaccgc
    25921 actgcgatct ggtatctcaa ccgtacttgg aaagagcggt tgttcgttgt tagttaagga
    25981 gcttgagaag aggagagagg gcgagactga agagatgcta agattctcgt cttctcgtga
    26041 gtgaagcgaa ctctcgaatg cgctctttcc cggcccttat caacaaccgt tcttatcact
    26101 cgaaagaatt gagtagttca ttcagaaagt ctgctactgt tatggtcaga tcatcctgga
    26161 ctaatcccaa atcgatattc acaccggcgt gatttttgtc tttggcatgg tacagccgaa
    26221 ccggcacttc ggaaagctgg agtttctgag cgaagtcact ggaaatttcc tctgactcta
    26281 tacgttcgcc ggcataaact atcaggaaag gggaaattga ttttcccgga gcaacatgat
    26341 tgattggcga agcatcatcc cataacggta cgcaggtgaa aggattatct cgggaataga
    26401 taacggcgcc agaagaagtt tttatctttt attgaaaaca ccgaaaagcc aatgattcat
    26461 acagtcggaa ttaaaggcgt ttccggtaag gaagagatgc cgaattgggg cccgacgcag
    26521 ccggaaggca ggaacaggag ccgctgttat tggtgagggg tgaatacgcc aacaaccaag
    26581 aacctggacg cgcagcgtcg gaacaaccgc tctcctgctc tttccaagga cggctcttaa
    26641 cgctcacttc acaggaccag cgattctccg ttggcgatct ccctcgatcc ggccggtaaa
    26701 ccggaaaagc tttccacagg ttcgctcgcg actattttaa gaccttccct ttcgtaaagg
    26761 taaagtccgt ggtcgaggtc gtttttctcc gagttcagtc tcaatatcca gatactttcg
    26821 aaatccgtca aaaaagcgtt gagcgaagtg tatcgaaaat tgtccgccac gaacccggca
    26881 gccctgccaa gtgcctcggc agggctgaac ctttccatat gactcaaaac aagttcgaaa
    26941 tatctctggg tatcgatcgt tccgggagca ctggcgaaat cgaagaccgt cccgttatgg
    27001 cagaaggcgt agtttttccc cttcacgaca ccgtagaaag ggtgaacgtg gtcgggattg
    27061 acagagtatc ccggggaagc cttacgtgcg tgtacgatcc ccagcctgct ttcgatcaac
    27121 ggtgggtcgg cttcacagat ggatcggaca gagttgtatc tcaacctttc tccgccggcc
    27181 atcgcaagac cgaagccatc gccgtgaggg ttgttcagac cctctctggc catgttccga
    27241 agtatatcaa cgatctcgag cgattctatt ttagtcgggc tcttgaaagc cagcatccta
    27301 cacatgatta catcctgggt atcgcattct cgacgctgtt tccgaccatg tccataaagt
    27361 atctctggag tcttacatcg tccgtcagct cgggatgaaa tgaagcggcc atgatgtttc
    27421 tctctcttac aagcacgggt gttcccccga aacaggccat cacttcgacc tctttgccgg
    27481 tggtcgcgat tttaggcgct cttataaaaa cagccctgaa gggaacgtct ccgaagtagc
    27541 tgacgtacag gtcggcctcg aagctgtcga cctgtcttcc gtaaccgttt ctctcgatcg
    27601 tgacatccag aacgcccaga gtgtcctgcg cggggtagtt ctctattccc ttcgagagga
    27661 ggatcatgcc ggcacaggtg gcgaagaccg gcatgcccga atttatcctt tctcttagtg
    27721 gttcccagat tgcaaatctc ttcagcagct tcgtcatggt ggtcgattcg ccgccgggca
    27781 ttatcaaagc ctcgactgtt ttgagcgcgt cgctatctct tatccatacg gcctcatgac
    27841 cgagtttctt aattacagcc aggtgctctt gaatatctcc ctgaataccg aggactccga
    27901 ttttcaattt ttaccagcct ctctcttcca tacggacttc gagcttgccg atctccaggc
    27961 cttccatggc gtctcccaca tcttcagaga tctccgccag tctgtgcggg tcgttgtagt
    28021 aaagaa
//
LOCUS       DAOOLY010000003        25484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_1811_length_25484_cov_5.334697, whole genome shotgun sequence.
ACCESSION   DAOOLY010000003 DAOOLY010000000
VERSION     DAOOLY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 25484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25484
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1811_length_25484_cov_5.334697"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1016)
                     /locus_tag="PLD15_00300"
     CDS             complement(84..1016)
                     /locus_tag="PLD15_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPB62363.1"
                     /translation="MRVERLLGITIVLLGRRRVPVRELAERFSVSCRTIHRDLDALSM
                     AGIPVVTVRGSGGGVEIMEGYRLSSTLFNQDELLSSIVALEGLGNALDSDSITSALEK
                     IKGLLSREQQEKIEGRRSLFLDISPWFSSQIEEHMLSQIREAISKSLLISFRYMNNKG
                     QESRRRVEPMSLVFKGYTWYLYGYCLLRDDYRFFKLSRMDELEITTESFTRREKELEK
                     KRVENSIDWQEKLERIVIKISSKVRARAVDTFGRKAIKFIPDGTGLVEVFWPLDEWLY
                     STILSFGADIIVIEPGSLKDEIKKRLISMLNRYT"
     gene            1196..2395
                     /locus_tag="PLD15_00305"
     CDS             1196..2395
                     /locus_tag="PLD15_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62364.1"
                     /translation="MLVKRKKGIIDTGILIGLSLAAVVGLMVYLFPTVTWPSPGGRYD
                     VGLKIIELEDKSRQDPFADVTMSRRLVVDVWYPAADTAGNRRSPWFRNAPLFINSLAS
                     NYELPAIIFQHLRTVKSNSYLEAEPLMIEGGAPVVIFSPGTPAIVPLYFSFAEYLASR
                     GYVVIGIEHPYGAAVVEFSDGTQAHFSRERAEQFPGADSFEEGVRLGMELMAGDFSFV
                     IDELENMADGDRLPVDFFDTDRIAVFGHSGGGGVIQFLSQREKRIDALVSFDPALFVM
                     TDDELKAGMDIPALVMETGKWLEREEAGNLYLLTNSGEVKPYHLKLPNANHPDFAMLD
                     HLSPLAHYLGFTGTFMTGGGEEYLYNSISSFLEFAFDERSEEEFLETLLSRKDVQLFI
                     GDREINR"
     gene            complement(2385..4472)
                     /locus_tag="PLD15_00310"
     CDS             complement(2385..4472)
                     /locus_tag="PLD15_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB62365.1"
                     /translation="MINRKISISIIVLFAVATVVMGLFLLKLEINVSSEFLLPEESPS
                     RENMRNMENLFGKEKQIIIVVKTDGLFEKENSLKIYNFLQKLSSIEGVVSYNSYQDAA
                     KVSLIGGTNVQPYFEEGLPAENAREILDNPLYLDNLLDEGGEVALIPINVADGADIKA
                     ILKVAGDSLEGMEFHASGEPIVDEELNSSILSLMVVYPPILFGLICFIYYLRLGSVRA
                     AIIPSILSIIAALWTYGSGVMIGFDINILTSTVGLFIVIISSSYGLHFIDRYITNRRV
                     LERHLALKRTIREELVPVFLSALTTAVGFLTFLVSSLEAFRQLGIMVSLGIMLSSALV
                     LVALPAFLFFVDLPAVERKLRFKGGTRERTAKIDRIVLVAIIVFAVVSPFLISRIENN
                     FDQFDYFRKSSTIVKSAETIREEFGWNMPFYVVLNKNSLFTGGDAQTLSKLIGDIEEL
                     SQVRGVSSIMDVSKAFNIPLPLLQLSARSGDLPVQQYMVGNSIRLLVKTPNTDAVSSQ
                     RLEAELKELLEDYPQYSPYIASPLLIISGVNSEILASQVSTIVWALIVITLLLIVVFR
                     SFKLAIVSVLPIAMSVFFNFSYMAILGIKLEISTAIVAGILLGMTIDYAIHLINRFIE
                     TKDIYRARQEVKPAIFSNTVALAAGFATLVFAPLKLFSGLGLLLAIGMVTGAILTLTL
                     IPHIIRSRKKTTG"
     gene            4596..5081
                     /locus_tag="PLD15_00315"
     CDS             4596..5081
                     /locus_tag="PLD15_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB62366.1"
                     /translation="MSGGGKEISPENILEAVFDLIRNFTKFFSFSIEAEEMKTIELYI
                     LLYVALKGPQNMSSLAKEYSMTKSNITLLVDDMEKKGYLARVRSEEDRRVIMINLTER
                     GTALYRSFLSSFSELIAIFVKNVSPEDLAIITEGFERITGIVIEKWYADSVGDRGSDR
                     P"
     gene            5078..6073
                     /locus_tag="PLD15_00320"
     CDS             5078..6073
                     /locus_tag="PLD15_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB62367.1"
                     /translation="MILVTGSTGHVGNVLVKELAGQGESVRAIALPGEDVSMIDLPGV
                     EILRGDIRDRDFVIAACKGIDTVYHLAALISIMPTMKKQVRDVNIGGTENVIHACKAQ
                     RVGRLIYTSSIHAFTELEPGSFIDENVPFNPARTSGVYGKSKAEAVLNVLTAAREGLN
                     AVVLCPTGIIGPFDYRLSEMGNMIRLFASGKLRVGIEGSFDFVDVRDVVKSEIEASKK
                     AKAGEVYILGGEAVSIRELTDILHQITGIKRVDTFLRSTTAYVLSALTTLYYMLSKSK
                     ACFTPYTVHTLTRNYRYSHEKAAKALGHTPRKVSESLRDAVEWLSGYGIIRYAAR"
     gene            complement(6107..6943)
                     /locus_tag="PLD15_00325"
     CDS             complement(6107..6943)
                     /locus_tag="PLD15_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB62368.1"
                     /translation="MQILFLGTAAYEGFPNPFCEGDNCSAVRRGPRESFRLTSAALLN
                     NDLLIDFGPNIMAGTQKSNASLFNVETLLVTHSHSDHLYLPNFGFRKMPYNRSYERLP
                     VMTVLSNSTVLGRIAEATYYDPEKTVLLEASPFEKAKVNGYTVTPVPAVHKVAEGETP
                     LIYIIERDGVSLFYATDTGPLDKGCMERISKTLAKPVDMIALDATMGLVESNIFPYHH
                     SLYQLLDTVRAMRGLNILNDDSRIYAHHFSHAANPPHEELEKLYASHGISVTFDGLRV
                     EL"
     gene            complement(6990..>7831)
                     /locus_tag="PLD15_00330"
     CDS             complement(6990..>7831)
                     /locus_tag="PLD15_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB62369.1"
                     /translation="GEAGCGYENRGGVRMRITVLCNDKALEGFRSEHGISLLVEDRDK
                     IFLFDTGSTDVAVHNAIKMGIDLSKIDAIIISHGHDDHLGGLANVLRLTGSKHVYAGN
                     GAYLHKYSDTTFGSPIYGREFCESLGASFNFVTEDFELYDGVFLLTAVPFETGERPQE
                     KFNILVNGQRKRDLFEDELTLLIVINNRGTVITGCSHRGIGNILNRAGRYCHVKNVVG
                     GLHLAHKSLAELDGDVDYLKRFNVESYHIGHCTGDKAIETIMNRVPATVEELMAGQRF
                     ETF"
     assembly_gap    7832..7931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7932..9463)
                     /gene="ftsH"
                     /locus_tag="PLD15_00335"
     CDS             complement(<7932..9463)
                     /gene="ftsH"
                     /locus_tag="PLD15_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB62370.1"
                     /translation="MDKRFRYLLYYSLGVLILMTIFNFFFLNHINTEEITYSRFQQML
                     EAGEISQVSIESNRILILPSEETGIEKVLYTGIINDPGLVERLNSAGVEFRAPIETES
                     RGFFTSWILPLLIFALFWIFISRFLQKRMGSGVMSFGKNNAKIYAEKSTGVTFEDVAG
                     QDEAKASLKEIVDFINNPEKYAEIGAKLPKGALLVGPPGTGKTLLAKAVAGEAGVPFF
                     SISASEFVEMFVGVGASRIRDLFKQAEEKAPCIVFIDEIDAIGKTRDSVIGGNDEREQ
                     TLNQLLAEMDGFDSSRGVVILAATNRPEVLDKALLRPGRFDRRIVVDIPDLEGREAIL
                     KVHLRKVKFDLEMDINAVARSTPGASGADLANMVNEAALLAVRRRKKIIGQGELEEAV
                     EVVIAGAKKKSIVSKKEREIVAYHEVGHAITAAALKGNAPVHKITIIPRTTGSLGYTM
                     QLPQEERFLLTREELLDEIVIMLGGRAAEEITFGSVSTGASDDIERATRLARSMITIY
                     GMS"
     gene            complement(9456..10013)
                     /locus_tag="PLD15_00340"
     CDS             complement(9456..10013)
                     /locus_tag="PLD15_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB62371.1"
                     /translation="MPKIIRNVEEKILEAACELFQENGYRNCDMKSIAARAGTAVGNI
                     YRYYRSKDELFVESMKSSLMNEIDQLERSVDSSQSAREKLLKFLQGLYGIMEKKIDLF
                     EEYVTERETLPGINLEILLEERLMEILSELEPIPEGCEERAATCLLVSILVMLRRFPG
                     EGEKNLRFMEFMLNGLLMKREVRDG"
     gene            complement(10110..10943)
                     /locus_tag="PLD15_00345"
     CDS             complement(10110..10943)
                     /locus_tag="PLD15_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPB62372.1"
                     /translation="MITQFIGDVLSYEFLRNAMIAAILVSTLTGVFSSVVVLRKIEFI
                     GDGAAHATFGGIAFGLLLGANYIVMAALTAVVFAVAVSYFTRRNRLSESSVIGMLLPL
                     SMSLGVIALSFVRGYTPDVMGLFFGNILLVTAADVWLLAGANLGAIIFFSLFFREILY
                     YAYDEKMARHYGVPVAFVHYGTLIGISLSVVSSVKIAGIILVTAFLIIPAVSARLLAR
                     SLRSMISISVVLGVTASVLGMFFSYVLNMPPGPVIVVLLFVQFLAILSVKKLLGSKEN
                     D"
     gene            complement(10940..11683)
                     /locus_tag="PLD15_00350"
     CDS             complement(10940..11683)
                     /locus_tag="PLD15_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPB62373.1"
                     /translation="MERDRVLQVEDLTYKIGSAWILRGVSFSLSRGEFAGIIGPNGAG
                     KTTLIKAIVGDLSDYSGSIKVTGRVGYLPQNPERSRDFPISVREVVAMGLYKENGPFK
                     RFSKSEWNTVDTLLKTTGIEGLGRRRAGTLSGGEYQRLMLARALASNPELLILDEPEA
                     GVDEMGKSSFYRLLEKLKNEKNMGILMVSHDIGMVFEACDTIMCINKTLHCHTSADMI
                     SPEQLKSAFSTDLDLFIRSRDHFEREHSV"
     gene            complement(11670..12557)
                     /locus_tag="PLD15_00355"
     CDS             complement(11670..12557)
                     /locus_tag="PLD15_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HPB62374.1"
                     /translation="MRRFIAFFVLILLVATSAIALKVTATINPYYMIVRDIVGDRAEV
                     SLLIGPGQNPHVFSPKISDVKRLNEADLVVANGLELEAFLESTLEELSRKGKRVLLAG
                     ESVPPSLLSEEEDGHGETDTLEHSHSINPHVWLDPLLLVDHIIPAIVEALTEIDPESG
                     SYYGENAEKISGRLMYFYGEAGMYLERFRGGVVIVAHPSFSYFFDRFGIVLEPVLEGV
                     GDEPSIQELKRLVDFARSEKVIGIFSEYQQSKRPVEILAGEASVGSGSLDPLGISRAD
                     IIDHFRWNLEEMKKVFGER"
     gene            complement(12554..12967)
                     /locus_tag="PLD15_00360"
     CDS             complement(12554..12967)
                     /locus_tag="PLD15_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPB62375.1"
                     /translation="MLVLKRLTTLRKSVLDIIDSSSAPLSADNIHGKLQARPDLSSVY
                     RSLSFLEERGLIRSVSFECDTRYYFGAGKEPVHFIHCKRCHRTESFRGCFAAELEESI
                     RDDHDFTITDHVFYFIGICGDCKRKMIEELEGGKL"
     gene            complement(13103..14242)
                     /locus_tag="PLD15_00365"
     CDS             complement(13103..14242)
                     /locus_tag="PLD15_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62376.1"
                     /translation="MGRKLLRIFGLAVVLCMLLGSSTLLSQSYYLGTSANGYQVPRDG
                     GLKLEPVPGKENWYAITIDFNEDNRDPMYDGHYYKVTDGTWNADGCWGVDNYAFQPAP
                     VKKLKDGTVVGLGSIYIQENCKLQILFDANTKTIYDDYLQRFPTPRIYGDFNEAMGRG
                     ANWSMTDESALVLTDPNADGVFNGFYKLPAYTGSGDGYMMVTVLSTRFNTQYYFFGAV
                     EQYKFDGTPAGMGMASYLKPLTDTIYEFQYDGSTHVTTFAECVTDQVVQLPSPVVYGD
                     FNGWNIEGPRAVVLAKDGENTYSTVLKLPAYTGEGSGYMVLVCLSKKFYNDQWGMRWG
                     AEEQYIFDGTRAGMGQVSYLKPAAETSYKLTYNSLTHVTTLEEAK"
     gene            complement(14306..16003)
                     /locus_tag="PLD15_00370"
     CDS             complement(14306..16003)
                     /locus_tag="PLD15_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HPB62377.1"
                     /translation="MERAAIYHISEHNYAYAVDGDTLAVRLRAKKNDLERVVVHYKNL
                     YDHTSRPEELSMEKILSDGTSDLYEARIRVKEKRFKYYFELFSSWKRVFYTSDGFLES
                     VDEKNCFFYPYINDDDIFRPPSWAEGEIIYQIFVDRFYDGDPSNNPPGTIPVGTPPGR
                     ESYYGGDLEGVILKLDYVAGLGAKIIYLNPIFLSNSYHKYDIIDYYSIDGAFGKTEDL
                     VRLVERAHERGMRVVLDGVFNHCSSSNPLFLDVLERQSESRYRDWFCIERFPIDATGK
                     NYDTFAGLVPGMPRLNTCNPEVIRYITDIALHWTKLLKLDGWRLDVADEVSHALWINL
                     RRELKALSPDILLIGEIWNHSSRWLQGREMDTATNYKFMRALHEFVADKIEARSFWER
                     VSANKMLYRSCLYNYLVNLVGSHDTTRCRTILGSDELHILAMAVNLTFEGMPLIYYGD
                     EVCMEGGFDPDNRRAMKWEDVESRCARKIRELSRFRAGSEILKKGSLIPIEAGRRVLA
                     FYREFEGERLYFLANFDVRASVLEGPWRDAQVILGEGSLEGGRLALESGRYVLLGDK"
     gene            complement(16016..16849)
                     /locus_tag="PLD15_00375"
     CDS             complement(16016..16849)
                     /locus_tag="PLD15_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB62378.1"
                     /translation="MGWTKAKTGRVFIHLFLALLAVITVIPFFYVVIISFGKNVIDTG
                     AFAITELTLDNYTRLFSETRFMSWILNSLLLAAVTMVVAVLVVSICVYAFSRLRFYGR
                     VGLFNFILLVQIFPLTLSMVSIFKIFVALGLLNKLEGLILADSAFASAGLVILAKGYF
                     DTIPYSLDEAAMIDGAGKFKILTRITLPLAKPMIAIVAIQSFVIAYNEYVIASTVMTQ
                     KLEAMPLAVGLQSMIVGQFGINWSVYCAGAVLGSIPMLVLFYSLQKYFIGGLTEGSVK
                     A"
     gene            complement(16873..19185)
                     /locus_tag="PLD15_00380"
     CDS             complement(16873..19185)
                     /locus_tag="PLD15_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB62379.1"
                     /translation="MRGFRFVVIVQVALLLGARLFAAPDGGDSTIRIWFSHIEPENLA
                     MAAIAGEFTSLTGIEVEVVGRRSIFDAPRDLANNAELDDRPDIILMQAPDIGNMVASG
                     LIVPLEIDDDLRKRYLDATFEAFTYNNKCYGIGYSLDTSGLIYNRDLISESELPETWD
                     DFFRIAEALTLRNEDGKIVQYGTLLNPKDMWFNYPIIKDHGGYYYGKAPNGDYNPYDV
                     GLDNDCMLDYVNRMKELQKKGLTLANENGTESHISAEFANGRAAMILYGLWNASIYQS
                     MCVDYGIAPLPKGRNGEVPRPLATVQGFVVNRFTRNMEAVESFLEFVLRDENQQKLIE
                     AGNRAERRTGERNPCNISVIESDYVSKDPILSSLSAIGFNCEPFPNIPEGTIWYNYTS
                     TAFRTIFYGDKSGNPVDAKEKLTELANKIRGDVAVMNKVPEKIEIPTGYLVFFASLAA
                     AGLAFLLVWRRRSAAPGEIAERYGRKESIVAWLMLAPLFFLLSMFYIFPVFHNIYLSL
                     TNYSGVNLRDYGIVGLSNYVEIFSTGINGLVSMIIWTVAFAATVVGLSFLAGTVLAVV
                     LDKVKVRIAKIYKIVFILPWVVPSVITLLMWRGMLESDGLVNRLLGIMGMPPVPWLTS
                     SFVAKLSCIFVMSWFSFPYFMVVSSGVLKSIPRDYYEVAKIAGAGNAFIFFKITVPLV
                     FKALFPMMIMSFIMQFNQFGVYLLTQGGPPGDVLGSPGSTDLLITYVFNTAFNTKRYS
                     LAAAYSVIIFCFVGLFAFVSLRIGKRKIYE"
     gene            complement(19211..20284)
                     /locus_tag="PLD15_00385"
     CDS             complement(19211..20284)
                     /locus_tag="PLD15_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB62380.1"
                     /translation="MFRELDRYKPDIDKTIAMMGKTYEDALSHRGGNVTIRALPKCDV
                     EHYSRLDLTRYNYRNDIDLYARDLCGLMSESFEKRRAVDDNMIPSVSPVLGIGDYSAF
                     VYGNIEFQKDTSWSKPVLGEIRDFRRLPEIGTSFWYGNFLNICEEILKLSSGAGIPFM
                     KGFFSPLDLAAALRGEAIYTDFYEAPEELHELLDYCADATIKFAEDVYALARRYLGGS
                     KYGMWLIDKCIYMSEDIACMISPRLYRKFCAPHTQRVIDHFGTGHMHCHSRALYLVKE
                     ICSLEKVASLWIATDPNQPRPIENIGRLVEDSRGVCLAIDCDSFDEIVANMEAMRRGN
                     VSICLPVEGVDEAVRVTEEFKTL"
     gene            complement(20300..21130)
                     /locus_tag="PLD15_00390"
     CDS             complement(20300..21130)
                     /locus_tag="PLD15_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB62381.1"
                     /translation="MNELKILRELGFSLNEAKCYIALHKSSPMTGYEVAKKAKITRTM
                     VYDILQRLERKGSVNVIEGNPKLYSAVSYKKLIASLRKDYTEKLDDLESSLEKITREV
                     DADNYILNLSNHAEMLNLIANAIKEAKREIYLSLWDSEAALFENELREADGRGVKIYI
                     FSFCKMPFDFGVQFTYKMKNANMNFPRRRIIGVFDREFMVMGEGNSSINEIGITTRNI
                     MLMEMSIDQMLLDIILLSTLRNGGYIWDGIDVGEYYDNVQRFFKSIELPADLPLRVDE
                     "
     gene            complement(21514..23394)
                     /locus_tag="PLD15_00395"
     CDS             complement(21514..23394)
                     /locus_tag="PLD15_00395"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPB62382.1"
                     /translation="MSLKRLLYGSIAPRGWLFEEAARNLKGLVGELDDLVPGIIRDDE
                     IYGRDRLTGAVKSKDLGTVAQDQEWEVQYLWWNSESQSNWLDGLIRHAYLVGNDEYID
                     KAKKYLRRVLMAQDDDGYIGIYAGDLRFDFKGENGELWAQATFLRAALGYYELTGESE
                     VLERVMKAFDLTMKAYSERNPFDLEKAYAGASHGLAFTDVCHGLFGLTGDKKYLRFAG
                     RLVDFYNRARLSEEDIREDNLLDYAYRFKGHGVHTYEGLRSLIIASMPDGRYNRAIEA
                     YLHRLNFVTTPSGAPIGDEWIGGRSADATETGYEFCSIQELLHSYLTLLETTGDLRWA
                     DRAEWLYYNAAHGAVNPRKSQIAYCKSDNSYAMNGHVGGMGSENDLRYKYSPVHQDVA
                     VCCAPNAGRVLPYFTGSMYMKSRHGLLVALYGPSRVETSIDGIDIALEQITSYPFDLN
                     IELLVTTAVRSNFTILLKKPSWVEKMKVEANGAKVEDCVDSIAVSKDWKDDRILIEFT
                     GRITERLDLKGDVYYSYGPLLYAMEIESAEKPGRLYGKGFEDNLFLPEDRSFLELCAT
                     EKTLESLAVSNDKHSRDFIDPPLMRGKFLDRSSGEEKTVFLKPIGSTILRKTTFQKNV
                     RL"
     gene            complement(23398..23952)
                     /locus_tag="PLD15_00400"
     CDS             complement(23398..23952)
                     /locus_tag="PLD15_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB62383.1"
                     /translation="MWQGLNGLELSLMNWLVSPIEGAFMDLLMSFFLFLARGSLVWIL
                     LTVFLLFDKRYRRTGYLTSAGLAISLLLSFVILKPILARTRPFLLLEGYSLMFPLPET
                     YSFPSSQVTVAFAYVAMTWKSVKSLRIPVLAIASLVSLSKIYVYFNYFSDVVAGIFLG
                     VFCGWFAVKLFEKYTVVRPELPER"
     gene            complement(24047..25102)
                     /locus_tag="PLD15_00405"
     CDS             complement(24047..25102)
                     /locus_tag="PLD15_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPB62384.1"
                     /translation="MLKKFVLIGVLVVLSAMVFAGIIGHVQEVDKYGNVHTDIPVEAV
                     VYAAIEAGDTVSIRIGETTIVVPFVTTYGDVDRGSALARYSGDHVLLAINYGNFAQTY
                     GLEAGTMIRMALVEKGAYKEELEIRHLVRTDLRSDYASDEVFANFREITIGNIAPHRL
                     YRCSHPSIDDPRAPYAAALVEAAGIKTVINLSDSDEELAASYAYSQYYKSIGEAGNLI
                     NLNMGVDMLSDDFSQKLKSGLLFMIEKEPPYLVHCVEGKDRAGIVGALLGAIMDAKTG
                     EIYADYVKSYENYYKVAPGTLAYEAVEGIIADILVAMNDGNPVDDSNIKEVALNYLKT
                     KIGLSDVQIAALQAKLK"
     gene            complement(25249..>25484)
                     /locus_tag="PLD15_00410"
     CDS             complement(25249..>25484)
                     /locus_tag="PLD15_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62385.1"
                     /translation="YKMLMWGMLRRILLLNFEKTGECEFKEAAEKVYDQMKEMSDALE
                     GELDYVVIPIKKLVQIQLMSGLYAALYAIDKNS"
BASE COUNT         5715 a         7018 c         6474 g         6177 t
ORIGIN      
        1 cagaaagcta acatcttcac ctcgatcacc tccgagagaa tactatcaga aataatatga
       61 cataagtgag tcatatttgg aatttatgta tacctattca acatagatat caatctcttt
      121 tttatttcgt ctttgagaga cccgggctct ataacgatta tatccgctcc aaaagagaga
      181 atcgtcgaat acaaccactc gtctagcggc cagaaaacct ccaccaatcc ggtgccgtct
      241 ggtataaatt tgattgcctt tcttccaaag gtatctacgg cccttgcccg aaccttcgag
      301 gagattttta tgactatcct ttccagcttt tcctgccagt cgatggagtt ctccacacgc
      361 ttcttttcga gctctttctc cctgcgcgta aaactctccg tggttatctc cagttcatcc
      421 atgcgggaga gtttgaagaa cctgtaatcg tccctgagca ggcaataccc gtaaaggtac
      481 catgtgtagc ctttgaagac gagcgacatg ggttccactc ttcttctcga ttcctgaccc
      541 ttgttgttca tgtatctgaa ggaaatgaga aggcttttcg aaatggcttc cctgatctgc
      601 gaaagcatat gctcctctat ctgactggag aaccacggcg agatatccag aaagagagac
      661 cttctcccct ctatcttttc ctgctgttct ctcgagagaa gcccttttat cttttcgagc
      721 gcgctcgtta tggagtcgct gtcgagagcg tttcccagtc cttccagagc gacgatcgag
      781 gatagcagtt cgtcctggtt gaagagtgtg ctggagagtc tgtacccttc cattatttcg
      841 acgcctccgc cgcttccgcg aaccgtgacc acgggtatgc cggccatcga cagcgcatcg
      901 aggtccctgt gtatcgtcct gcacgagacg ctgaacctct cggccagttc gcgaaccgga
      961 actcttctcc tgccaagcaa gactatggtg attcccagta acctctccac tctcataaag
     1021 atctctccag atgaacggat ttcgtgagcc acaactgtat tctctcatca ttttacagga
     1081 tttgaatcgc ggttcgatgt ggccgcaaat tcaccggcca tcacggtaat ttcggtgatg
     1141 actctttcga ggcgctgttt tccgatattt tttatgcgat aattgaaccg aggtgatgct
     1201 tgtgaagaga aaaaagggaa ttatagatac cggtattctc atcggactct ctctggcggc
     1261 cgtcgtcggt ctgatggttt atctctttcc gaccgtgacc tggccctctc ccggcggccg
     1321 gtacgatgtg ggattgaaga taatcgagct cgaagacaaa tccaggcagg accccttcgc
     1381 cgacgtcacc atgtccagga ggctggtcgt ggatgtctgg tacccggccg ctgatacggc
     1441 gggcaacagg aggagtccct ggttcaggaa cgcccctctg ttcataaaca gtctggccag
     1501 caactacgag ctgccggcga ttattttcca gcacctcaga accgtgaaat ccaattccta
     1561 ccttgaggcc gaacctctga tgatcgaagg cggcgcaccc gtggtgatat tttcacccgg
     1621 tactccggcc atcgtacctc tctacttctc tttcgcagag tatctcgcca gcaggggata
     1681 cgtggtgata gggatagaac acccttacgg ggcggccgtc gtggagtttt ccgacggtac
     1741 gcaggcgcat ttttcaagag agagggccga gcaatttccc ggcgcggata gtttcgaaga
     1801 aggagtaaga cttggaatgg aactgatggc cggcgacttc tccttcgtga tcgatgagtt
     1861 agagaacatg gccgacggcg acaggcttcc ggtagatttt ttcgataccg acagaatagc
     1921 ggtcttcggt cattccggcg gtggaggcgt gattcaattc ctctcgcagc gagagaagag
     1981 gatcgatgcg ctggtctcct tcgatccggc actcttcgta atgaccgacg atgaactcaa
     2041 agcggggatg gatataccgg ctctcgtgat ggaaacaggc aaatggctgg agagagaaga
     2101 ggcggggaat ctttacctgc tgacaaacag cggtgaggtt aaaccctatc acctcaagct
     2161 acctaacgcc aaccacccgg attttgcgat gctggaccat ctgtcgccac tggctcatta
     2221 cctgggcttc accggcactt tcatgaccgg cggcggcgag gagtacctct acaactccat
     2281 aagcagtttt ctcgagttcg ctttcgatga gagatccgaa gaggagttcc tggagacttt
     2341 actctctcgc aaagatgtgc agctcttcat aggagacagg gagatcaacc ggtagttttc
     2401 ttcctgctcc tgattatgtg cggtatcagc gtgagcgtca gtatcgcgcc ggtgaccatg
     2461 ccgatggcaa gaagcaatcc cagccctgaa aacagcttca ggggagcgaa tacgagagtt
     2521 gcgaagcccg ccgcaagcgc tacggtgttg gaaaatatag ccggttttac ttcctgcctg
     2581 gccctgtaga tatctttcgt ctctatgaac ctgttgatga gatggatcgc gtaatctatg
     2641 gtcattccca gcagtattcc cgcgacaatc gcggtagaga tctccagttt aatgcccagt
     2701 atagccatgt aagagaagtt gaagaacacc gacatggcta tcggaagaac cgacactatc
     2761 gccagcttga aggatctgaa gacaactatc aggagcagag ttatgactat caaagcccat
     2821 acgatcgtac ttacctggct ggcgaggatt tcgctgttga caccggatat tatcaagagg
     2881 ggcgaggcta tgtatggaga gtactgaggg tagtcctcga ggagttcttt caattcggct
     2941 tcgagccgct gagagctgac ggcatcggtg tttggagtct tgaccagcaa tcttatcgag
     3001 ttgcccacca tgtactgctg aacaggcaga tcgccgctgc gtgccgacag ctggagtaat
     3061 ggcagcggta tgttgaaggc cttcgagaca tccattatcg aggaaactcc cctgacctga
     3121 gaaagttctt ctatatcgcc aatgagtttc gacagggtct gggcatcgcc accggtgaag
     3181 aggctgttct tgttcaaaac gacgtagaaa ggcatattcc agccgaattc ctctctgatc
     3241 gtctcggcag atttcacgat cgtggagctc ttcctgaaat aatcgaactg gtcgaagttg
     3301 ttttctattc tcgatatgag gaagggcgaa acgaccgcaa aaacgattat cgccacaagc
     3361 acgattctgt cgatcttcgc cgtcctctcg cgtgttccgc ctttgaaccg gagctttctc
     3421 tccaccgcgg gaagatctac gaaaaagagg aaggccggaa gcgccaccag gacaagcgcc
     3481 gagctcaaca tgattccgag cgagaccatt atacccagct gcctgaaggc ttccagactg
     3541 ctgacaagaa aggtcagaaa gccgaccgca gtcgtgagcg ccgagaggaa gaccgggacc
     3601 agctcctcgc gtatggtgcg cttgagagcc agatgtcttt ccaggactct cctgttcgtg
     3661 atatacctat ctatgaaatg gagtccgtag gacgaggaaa ttattactat gaaaagcccc
     3721 accgtagagg tgagaatgtt gatatcgaaa ccgatcataa cacccgatcc gtaggtccag
     3781 agggcggcga tgatcgagag tatcgaaggg atgatcgccg cgcgaacgct tcccagcctc
     3841 aggtaataga tgaagcagat gagaccgaag aggatggggg gatataccac catgagcgat
     3901 agtatagaac tgttcaattc ctcatcgact atcggttcac cgctggcgtg gaactccatg
     3961 ccctcgagcg agtctccggc cactttcaga atggctttta tatcggcacc gtcagccacg
     4021 tttatcggta tgagcgcgac ttctcctccc tcatccagaa ggttgtccag gtagagcgga
     4081 ttgtcgagta tctcccgggc gttttccgcg ggaaggcctt cttcaaagta aggctggacg
     4141 ttcgtaccgc cgatcaggct cactttggca gcgtcctgat atgagttgta actcacgact
     4201 ccttcgatcg aggagagttt ctggagaaag ttgtagatct tgagagagtt ttccttctcg
     4261 aagagaccgt cggtcttgac cactatgatg atctgcttct cttttccgaa aaggttttcc
     4321 atattacgca tgttttctct ggaaggagac tcctccggga gaagaaattc gctggatacg
     4381 ttgatctcca gcttcagaag gaatagaccc ataaccaccg tggcaactgc gaacaggacg
     4441 attatagata tagagatctt tcgattgatc ataacggacg gccccctttg gtagaatttt
     4501 actactctta ccagaatagt aatgtttatg cacagtaaaa tttgttgata gttaaatgtt
     4561 taaactgtta taattacttc agaggaggtg cagcaatgag tgggggaggc aaggaaatca
     4621 gtccagaaaa tatactggag gccgttttcg atttgatccg gaacttcacg aagttcttct
     4681 ctttcagcat agaagccgaa gagatgaaga cgatagagct ttacatactt ttatacgtcg
     4741 cgctcaaagg gccacagaat atgtcttcgc tggcgaagga gtattctatg accaaaagca
     4801 acatcactct tcttgtggac gacatggaaa aaaagggtta tctggcccgc gtccgttcgg
     4861 aggaagaccg aagggtaatc atgatcaatc tcaccgaaag gggtacggct ctctacagaa
     4921 gctttctgtc cagcttctcg gagctgatcg ccatcttcgt gaagaacgtc agccccgaag
     4981 atcttgcgat catcaccgag ggcttcgaga gaataacggg catcgtcatc gaaaagtggt
     5041 acgccgactc ggtcggcgac cgtgggagtg atcggccgtg atacttgtga cgggttctac
     5101 cggtcatgtg ggcaacgttc tcgtgaagga gctggcgggc cagggcgaga gcgtgagggc
     5161 catagcgctc cccggagagg acgtatcgat gatcgatctt cccggagtgg agatacttcg
     5221 gggcgatata agggacagag acttcgtgat cgctgcctgc aaaggaatag atactgtcta
     5281 tcatctggcc gcgctcatat ccataatgcc caccatgaag aaacaggtga gagacgtcaa
     5341 cataggcggt acggaaaacg tgatccacgc ctgcaaagct cagcgagtgg gtaggttgat
     5401 ctacaccagt tcgatccacg ccttcaccga gctggagccg gggagtttca tagatgaaaa
     5461 cgttcccttc aatccggcgc gcacgagcgg agtttacgga aaatccaagg ccgaagccgt
     5521 gctcaatgtc ctgacggccg ccagggaggg actgaacgcc gtcgtgctct gtccgacagg
     5581 gatcatagga cccttcgatt acagactgtc ggaaatggga aacatgataa gactcttcgc
     5641 ctcgggcaag ttgagagtgg ggatcgaagg atcattcgat ttcgtcgatg tgcgcgacgt
     5701 ggtgaagtcc gagatagagg catcgaagaa agcgaaggct ggagaggtct atattctggg
     5761 cggcgaggcg gtctcgatcc gggagctcac cgacatactc caccagataa cgggaataaa
     5821 gagagtagat acctttctca ggagcaccac cgcatacgtt ctttcggccc tgaccactct
     5881 ttattacatg ctctcgaaga gcaaagcctg cttcactccc tacacggttc atacgctcac
     5941 gaggaactac aggtactccc acgaaaaggc ggctaaggca ttgggccaca ctcccaggaa
     6001 ggtttcggag tctttgagag acgccgtgga atggctttcc ggttacggga tcatcagata
     6061 cgcagccagg tgagctcttc ccggcccgct acgtctttgc ggggcttcac agctccaccc
     6121 tcaacccgtc gaaggtgacg cttatgccgt gcgaagcgta aagcttctcg agttcttcgt
     6181 gtggaggatt ggcggcgtgg gagaagtggt gcgcgtagat cctgctgtcg tcgttcagta
     6241 tattgagccc cctcatcgct ctgacggtat ctaaaagctg gtataaactg tgatggtacg
     6301 ggaagatgtt gctctccacc agccccatgg tcgcatcaag ggcgatcata tccaccggtt
     6361 tggcaagagt cttcgagatt ctctccatgc accctttgtc gagaggaccg gtatcggtgg
     6421 cgtagaagag cgaaactccg tccctctcga ttatatatat cagcggcgtc tctccctcgg
     6481 caaccttatg aaccgcgggc accggcgtca ccgtgtagcc gttgactttc gctttctcga
     6541 agggtgaggc ctccagaagc accgtttttt cgggatcgta gtaagtggcc tcggcgattc
     6601 tgcccagaac cgtggagttc gagagaacgg tcatcaccgg caatcgctcg taagagcggt
     6661 tatagggcat cttcctgaag ccgaagttgg ggagatagag gtggtccgag tgagagtgcg
     6721 taaccagcaa cgtttcgaca ttgaagagac tcgcgttgct cttctgagtg ccggccatta
     6781 tgttgggccc gaaatcgata agtagatcgt tgttcaacag cgccgccgag gtcaatctga
     6841 aagactcgcg cggtccccgc ctcacggcag agcagttgtc tccttcgcag aagggattgg
     6901 ggaaaccttc gtaggccgcc gttccaagaa aaagtatctg catacattcc tccgactgaa
     6961 tacgtgattc attgatctat attcgaagat taaaacgtct cgaacctctg gccggccatg
     7021 agctcctcaa cggtggccgg aaccctgttc attatagtct cgatggcttt atcgcctgtg
     7081 cagtggccga tgtggtaact ttcgacgttg aacctcttca ggtaatcgac gtccccgtcg
     7141 agttccgcca ggctcttgtg ggcgagatgg agtccgccca cgacgttttt cacatggcag
     7201 tacctgccgg cccggttcaa tatgttacct atacccctgt gcgaacagcc ggtaataacc
     7261 gttcccctgt tgttgatgac tataagaagc gtcagttcgt cttcgaagag gtcacgcttt
     7321 ctctggccgt tgacaaggat gttgaacttt tcctgaggtc tttcaccggt ttcgaagggc
     7381 acggccgtca gcaaaaacac cccatcgtaa agctcgaagt cttcggtcac aaagttgaaa
     7441 gaagcgccga gactttcaca gaactctctg ccataaatcg gactgccgaa ggttgtgtcg
     7501 ctgtatttat ggaggtaagc gccgttaccg gcgtaaacgt gtttgcttcc ggtgagcctc
     7561 agcacgttgg caagaccgcc caggtgatcg tcatgaccgt ggcttataat tatggcgtct
     7621 atcttcgaga gatcgatacc catctttata gcgttgtgaa cggcaacatc cgtcgatcca
     7681 gtatcgaaga ggaaaatctt atcgcggtct tccaccagca acgaaatgcc gtgttccgat
     7741 ctgaagcctt ccagggcctt atcgttacag agaaccgtga tcctcatcct tacacctccg
     7801 cgattttcat aaccgcaacc ggcctctccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn ngacattccg tagatagtta tcatgctcct ggccagtctc gtggctcgct
     7981 ctatatcgtc cgaggcgccc gtcgaaacac tgccgaacgt gatctcctcg gcggcccggc
     8041 ctcccagcat tatgacgatt tcatcgagca actcctctct ggttagaaga aacctctcct
     8101 cctgggggag ttgcatagtg taccctagcg agccggtcgt tctggggatt atcgtgatct
     8161 tgtgtacggg agcgttccct ttcaacgcgg cggccgttat cgcgtggccc acttcgtggt
     8221 aagcgacgat ctctctctcc tttttcgaaa ctatgctctt ctttttggct ccggcgatga
     8281 ctacctcgac cgcctcttcc agctcgccct gtccgattat cttctttctc ctgcgcacgg
     8341 ccagaagagc cgcctcgttt accatgttgg ccaggtcggc ccccgaggct cccggtgtgg
     8401 atctggcgac cgcgtttatg tccatctcga gatcgaactt aacctttcgc aaatgcacct
     8461 tgagtatggc ctccctgccc tcgagatctg gaatgtccac cacgatcctc ctgtcgaaac
     8521 gccccggtct caacagggct ttgtccagaa cctccggcct gttcgtcgcg gccagaatca
     8581 ccactccgcg cgaggagtcg aagccgtcca tctcggcgag caactggttc agggtctgct
     8641 ctctttcgtc gtttccgccg atcacgctgt ccctggtctt gcctatcgcg tcgatctcgt
     8701 ctatgaacac aatgcaggga gccttctcct cggcctgctt gaagaggtcg cggattctag
     8761 aagctccgac gccgacgaac atctcgacga actcggaggc cgatatcgag aagaagggca
     8821 cgcccgcctc gccggcgacg gccttcgcca gaagagtctt gccggttccc ggaggaccga
     8881 ccagaagggc tcccttgggg agttttgccc ctatttctgc gtacttttcc gggttgttta
     8941 tgaaatcgac gatctccttg agggaggctt tggcctcgtc ctgtccggcc acatcttcga
     9001 aggtaactcc ggtagacttc tctgcgtaga tcttggcgtt gttcttgcca aaagacatga
     9061 ctcccgatcc catacgcttc tgaaggaacc tgcttatgaa gatccagaaa agcgcgaaga
     9121 tcagcaaggg cagtatccag ctggtgaaga atcctctgct ttcggtctcg ataggagccc
     9181 tgaactcgac accggccgaa ttcaaccgct cgacgagccc gggatcgttg attattcccg
     9241 tgtagaggac cttctctata ccggtctcct ccgaaggaag tatgagaatc ctgttgctct
     9301 ctatgctcac ctgcgagatt tcaccggcct ctagcatctg ctggaacctc gaatacgtta
     9361 tttcttccgt atttatatgg ttcaggaaga agaaattgaa gatcgtcatc agtatcagca
     9421 caccgagcga ataatacaaa agatatctga acctcttatc catctctcac ctcacgtttc
     9481 attaaaaggc cgttgagcat gaactccata aagcgaaggt ttttctcgcc ttctccgggg
     9541 aagcgcctca acatcaccag tatgctaacc agcaagcagg tggctgcgcg ctcctcgcac
     9601 ccttctggaa tgggctccag ctcactgaga atttccatca acctctcttc tagaaggatc
     9661 tcgagattta ttcccggcag agtctctctc tcggtcacat actcctcgaa gagatctatc
     9721 ttcttctcca ttattccgta aagcccctga agaaatttca acagcttttc tctggccgat
     9781 tgcgaagaat ctaccgatct ttctagctgg tctatctcgt tcatgagcga ggacttcata
     9841 gactcgacaa agagttcatc cttggaccgg tagtatctgt aaatgtttcc gaccgccgtg
     9901 ccggccctcg ccgctataga cttcatgtcg cagttgcggt aaccgttctc ctgaaagagc
     9961 tcgcaggcgg cctcgagtat cttttcttca acgtttctta tgattttagg caactcgttt
    10021 ccacctcctt ttaatgaacc attgttcgct taaatgaatt atagttcatt aagctttgga
    10081 agtcaactat tcaggatgaa cctgtccact cagtcgtttt ctttcgagcc caacagtttt
    10141 ttcaccgaaa ggatggcgag gaattgaacg aacagcagga ccactatgac cggaccaggg
    10201 ggcatattca gaacgtatga gaaaaacatt ccgagcacgc tggccgtcac tccgagtacg
    10261 accgagatcg agatcatcga cctgagagat ctggcgagaa gcctggccga aaccgcagga
    10321 attataagga aggccgtgac caggattatc ccggcgatct tcacagagct cacaacgctc
    10381 aagctaatac ctatgagcgt tccgtaatga acgaaggcca cggggacgcc gtaatgtctg
    10441 gccatctttt catcgtaggc gtaataaagt atctccctga agaagagcga gaagaagata
    10501 atcgcgccca ggttagcgcc ggcgagcagc cagacatcgg ccgcagttac gagcagtatg
    10561 ttgccgaaga agaggcccat tacgtctgga gtgtagcccc tcacgaaaga gagggcgatg
    10621 actcccagcg acatcgaaag gggaaggagc atccctatga cgctgctttc tgatagcctg
    10681 ttcctcctgg tgaagtagct caccgcgacc gcgaagacca cggccgtgag ggcggccatc
    10741 acgatgtaat tggcgccgag cagcaggccg aaggctatcc ctccgaaagt cgcgtgtgcc
    10801 gccccgtcgc ctatgaactc tatctttcgc aacaccacaa cgctggagaa aacacccgtc
    10861 agggtgctca ccaggatcgc ggcgatcatc gcgtttctta aaaattcgta acttaaaaca
    10921 tctccgatga attgagttat catacgctgt gttccctttc aaagtggtct ctcgagcgta
    10981 tgaaaagatc gagatcggtg gaaaaggccg attttagctg ctcgggagag atcatgtcgg
    11041 ccgaggtgtg gcagtggagc gtcttgttga tacacatgat cgtgtcgcag gcctcgaaga
    11101 ccatccctat gtcgtggctg accatgagga tgcccatgtt tttctcgttt ttcagctttt
    11161 ccagaaggcg gtagaaggaa gactttccca tctcgtccac accggcctcc ggctcgtcca
    11221 gtatgaggag ctcggggttg gaggccagcg ccctggccag catcaatctc tggtactctc
    11281 ctcccgaaag cgttccggcc ctcctccttc caagtccctc tattcccgtc gtcttgagga
    11341 gagtatcgac cgtgttccac tcgctctttg agaaacgttt aaaggggccg ttttctttat
    11401 agaggcccat ggccactacc tccctgacgc ttatagggaa gtccctgctt ctctcggggt
    11461 tctggggtag gtatcccaca cggccggtga cctttatact gccgctgtaa tcactcaggt
    11521 cgccgactat agcctttatt agagtggtct tccccgcgcc gttcgggcct attatcccgg
    11581 cgaactcgcc cctggaaagg ctgaaagaga cgcccctcag tatccaggcc gagccgattt
    11641 tgtaggtcag atcctcaact tgaagtaccc tatctctctc caaagacttt tttcatctcc
    11701 tctaaattcc agcgaaaatg gtctattatg tcggctctcg aaattcccag cgggtcgagc
    11761 gaaccactcc cgaccgaggc ctctccggcc agtatttcca caggcctctt cgactgctgg
    11821 tactccgaga agatgcctat cactttctcg gatctggcga aatcgacgag cctcttcaat
    11881 tcctgtatcg acggttcgtc gcccactcct tcgagaaccg gttcgagcac tatcccgaaa
    11941 cggtcgaaaa agtaagagaa actgggatgg gccactatga ccacgccacc cctgaaccgc
    12001 tccagataca ttccggcctc tccgtagaaa tacatcaacc gtccggagat tttttcggcg
    12061 ttttcaccgt agtatgagcc gctctcagga tctatttcgg tcagagcctc gacgattgcg
    12121 gggattatgt ggtcgaccag caagagcgga tcgagccaga cgtgcgggtt gatcgaatgg
    12181 ctgtgctcga gcgtatcggt ttcgccgtgg ccgtcctcct cctcgctcag aagcgacgga
    12241 gggacagact ctccggccag aaggaccctc ttgccctttc tggaaagctc ttcgagcgtg
    12301 ctttcgagaa aggcctccag ctccagtccg ttcgccacca ccagatcggc ctcgttgagc
    12361 ctcttcacat ccgatatctt cggagaaaag acgtgggggt tctgccccgg tccgatcagg
    12421 agactcacct cggccctgtc cccgactatg tcccgcacta tcatgtaata aggatttatg
    12481 gtcgccgtga ctttcaaagc gatagcagaa gtcgcgacga gaagaatcaa cacgaagaac
    12541 gctataaatc ttctcacagt tttcctcctt ccagttcttc tatcatcttt ctcttgcaat
    12601 caccgcagat gcctatgaaa tagaaaacat gatcggtgat cgtaaaatcg tgatcgtccc
    12661 tgatcgactc ctccagctcc gcggcgaaac atccgcgaaa ggactcggtc ctgtggcacc
    12721 tcttgcagtg tatgaagtgg accggttcct tcccggcccc gaaataatac cgtgtgtcgc
    12781 attcgaagga gaccgatctg atcaatcccc tctcctcgag gaaggagaga ctcctgtaaa
    12841 cactcgaaag atcgggtctg gcctgtaact tgccgtgtat gttatcggcg ctcagcgggg
    12901 cgctcgagct gtctattata tccaggacgc tcttcctgag agtagtcagc cttttcaaaa
    12961 ccaacaccat ccttgccatt tgttagcgca aatcatttgc aatatattct cacacggccg
    13021 ctctgtgttg tcaagccggt ctttgcgaaa aaaaaaggcc cgggcggttt ttccggccgg
    13081 acctgtaaga tttcggattt ttctacttcg cttcttcgag cgtggttacg tgagtcaagg
    13141 agttataggt taatttgtaa gaagtctccg cggcaggttt tagatagctg acctgaccca
    13201 taccggcccg ggttccatcg aatatgtact gctcttccgc tccccatctc attccccact
    13261 ggtcgttgta gaacttcttg gagagacata ccagtaccat gtagccagaa ccttcgccgg
    13321 tgtaggccgg aagcttcaag acggtcgagt acgtgttctc gccgtccttc gcgaggacga
    13381 cggccctggg cccttctatg ttccagccgt tgaaatctcc gtatacgacc ggcgagggga
    13441 gctgcacaac ctgatcggtc acgcattccg caaaggtcgt gacgtgggtc gagccatcgt
    13501 actggaattc gtagatcgta tcggtcagag gcttcaggta actcgccatt cccatcccgg
    13561 caggcgtccc gtcgaatttg tactgttcga cggccccgaa gaagtaatac tgcgtgttga
    13621 acctggtgga tagaaccgtt accatcatgt aaccgtctcc gcttcctgtg taggccggca
    13681 gcttgtagaa gccgttgaat acaccgtcgg cgttcggatc ggtcagcacc agagcgcttt
    13741 cgtcggtcat gctccagttg gcgcctctgc ccatcgcttc gttgaagtct ccgtagatcc
    13801 tcggagtcgg gaatctctgg aggtaatcgt cgtagatggt tttggtgttg gcgtcgaaca
    13861 gtatctggag cttgcagttc tcctggatat agatcgaacc caggccgacc accgtgccgt
    13921 ccttcaactt cttcaccggc gccggctgaa aggcgtagtt gtccactccc caacagccgt
    13981 cggcgttcca cgtcccgtcg gttactttgt agtaatgtcc gtcgtacatc ggatccctgt
    14041 tgtcctcgtt gaaatcgatc gttatcgcgt accagttctc cttgcccggt accggctcga
    14101 gtttaagacc gccgtcgcgc ggcacctggt aaccgttggc gctcgtgccg aggtaatagc
    14161 tctgcgacag caaggtcgat gaacccagca gcatacacaa gaccacagcc agtccaaaga
    14221 ttctcagtaa ctttcttccc attttccctg cctccctttt gagttccttc gagttctgtt
    14281 gtcaccctgt cggattgacc ggatgtcatt tatcgcccag taagacatat ctcccgctct
    14341 cgagggcgag tctcccgcct tcgagagaac cctcccccag tatcacctgc gcgtctctcc
    14401 aggggccttc gagaacgctc gctctcacat cgaaattggc caggaagtaa agccgctccc
    14461 cctcgaactc cctgtagaag gccaagaccc tacggccggc ctctatagga atcaacgaac
    14521 ctttcttcag tatctcgctc ccggccctga agcgtgaaag ctctcttatc tttctcgcgc
    14581 accggctttc cacgtcttcc catttcatcg ccctgcggtt atccggatcg aaaccgccct
    14641 ccatgcagac ttcgtctccg taatatatca gcggcatacc ctcgaaggtc aggttgacgg
    14701 ccatcgccag gatatggagt tcatcgctcc ccaggatcgt tctacacctg gtcgtgtcgt
    14761 ggcttcccac cagatttacc agatagttgt acaggcagga cctgtaaagc atcttgttgg
    14821 cgctcaccct ttcccagaag gagcgcgcct ctatcttgtc cgcgacgaat tcgtgcagcg
    14881 ccctcatgaa cttgtagttg gtggcggtat ccatctctct cccctggagc caccgggagg
    14941 agtggttcca tatctcccct atcaggagaa tgtccggcga tagggccttc agctctctcc
    15001 tgagatttat ccagagagcg tgcgatacct cgtcggcgac gtcgagccgc cagccgtcga
    15061 gtttcaggag cttcgtccag tggagcgcta tatcggttat gtaacgtatc acctcggggt
    15121 tgcaagtgtt cagccgcggc atccccggaa cgagcccggc gaaagtatcg taattctttc
    15181 ccgtcgcgtc tatcgggaag cgctctatgc agaaccagtc cctgtatctc gattccgact
    15241 gcctctcgag gacatccaga aaaagagggt tcgaagagct gcaatggttg aagacgccgt
    15301 cgagaaccac cctcataccc ctttcatgtg ccctctccac cagtcgtacg aggtcttcgg
    15361 tctttccgaa ggcgccgtct atgctgtaat aatcgattat gtcgtacttg tggtaagagt
    15421 tcgagagaaa gatcgggttt agatagatga tcttagcccc cagaccggcc acgtaatcga
    15481 gttttaggat aaccccctcg aggtctccgc cgtagtagct ttctctgccc ggaggcgtac
    15541 ctaccgggat cgtgccgggc gggttgttgg aagggtctcc gtcgtagaac ctatcgacga
    15601 atatctggta tataatctcc ccctcggccc aggaaggcgg tctgaagata tcgtcgtcgt
    15661 ttatgtaagg gtagaagaag cagttcttct catcgacgga ctcgagaaag ccgtcggatg
    15721 tataaaagac cctcttccac gaggaaaaaa gctcgaagta atatttgaac ctcttctcct
    15781 tcacccttat tctcgcctcg tacagatccg aagtcccgtc ggatagtatc ttctccatgg
    15841 agagctcttc cggccgggaa gtgtggtcat agaggttctt gtaatgaacc acgactctct
    15901 ccagatcgtt cttcttcgcc ctcaacctca cggccagggt atcgccgtcg acggcgtagg
    15961 cgtagttatg ttcggatatg tggtagatcg cggctctctc catctctgtc tccttctatg
    16021 ccttcacgct tccttcggtc aacccgccta taaagtactt ctgaagcgag tagaacagca
    16081 cgagcatggg tatgctcccg agcaccgcgc cggcgcagta aacgctccag ttgatcccga
    16141 actgcccgac tatcatgctc tgcaacccaa ccgccagggg catagcctcg agcttttgag
    16201 tcatcaccgt gctggcgatg acgtattcgt tgtacgcgat tacgaaactc tgtatggcca
    16261 cgatggctat catcggtttg gccagaggaa gcgtgattct ggtcagaatc ttgaatttac
    16321 cggccccgtc gatcatcgcc gcttcgtcga gcgagtaagg gatcgtgtcg aagtaccctt
    16381 tggccaggat cacgagaccg gccgaagcga aggccgaatc ggccagaatc agtccttcca
    16441 gtttgttcag aaggccgagc gccacgaaga tcttgaagat cgagaccatc gacagcgtca
    16501 gcggaaagat ttgaaccagc agtatgaagt tgaacagccc gactctaccg tagaatctca
    16561 gcctcgagaa ggcgtaaaca cagatcgaga cgaccaagac tgccacaacc atcgtgaccg
    16621 cggccagaag gagcgagttc aatatccagc tcataaagcg cgtctcgctg aagagcctcg
    16681 tatagttgtc cagtgtcagc tccgtgatcg cgaaggcgcc ggtatctatg acgttcttgc
    16741 cgaaggatat aatcacaacg tagaaaaagg gtatcactgt aataacggcc agcaaggcca
    16801 gaaatagatg gataaatacc ctgccggtct tcgcctttgt ccagcccacc gtctcaccac
    16861 ctcgctcggg gattactcgt agatctttct ctttcctatc ctcagagata cgaaggcgaa
    16921 caacccgacg aaacagaaga ttatcaccga ataggcagcc gccagcgagt atctcttggt
    16981 gttgaaggcc gtattgaaaa cataagttat cagtagatcg gtcgatccgg gagagccgag
    17041 aacatcgcca gggggacctc cctgggtcag tagatagact ccgaactgat tgaactgcat
    17101 tatgaacgac atgatcatca tcggaaaaag cgccttgaac acaagcggca cggtgatctt
    17161 gaaaaatatg aaggcgttgc ccgctccggc tattttagcc acttcgtagt agtctctcgg
    17221 tatcgatttc agcacgcccg acgagacaac catgaagtag ggaaaggaaa accagctcat
    17281 gacgaatata cagctcaact tcgcgacgaa ggaactcgtg agccacggaa cgggcggcat
    17341 gcccattata ccgagcagac ggttcaccag tccgtcgctc tccagcattc cgcgccacat
    17401 cagcagcgtt ataacgcttg gcactaccca ggggagtatg aaaacgatct tgtagatctt
    17461 cgcgatcctc accttgactt tatccaggac caccgccagc accgtcccgg ccaggaagga
    17521 aagacccacg accgtggccg cgaaggcgac ggtccagatt atcatcgata ccaggccgtt
    17581 tatacccgtc gagaagatct ccacgtagtt cgaaagcccg acgatcccgt aatctctcag
    17641 attgacgccg ctgtaattgg tcagcgagag gtatatgttg tgaaagacag gaaagatgta
    17701 gaacatcgaa aggaggaaga agagcggcgc cagcatcagc caggccacga tgctctcctt
    17761 cctgccgtag cgctccgcta tctcgcccgg cgcggcggac ctccttcgcc agaccagcaa
    17821 aaaagcgagg ccagccgcgg cgagcgaggc gaaaaagacg agatagccgg taggtatctc
    17881 gatcttctcc gggactttgt tcataacggc cacatcgccc ctgatcttgt tggcgagctc
    17941 ggtcagcttc tctttcgcat ctaccgggtt gcccgacttg tctccgtaga agatagtcct
    18001 gaaggccgtc gaggtgtagt tgtaccagat cgtgccttcg ggtatgttcg ggaagggttc
    18061 gcagttgaag cctatcgcgc tcaaagacga aagtatcggg tccttgctca cgtaatcgct
    18121 ctcgatgacc gagatgttgc aggggttcct ctcgccggtt ctcctttcgg ccctgttgcc
    18181 ggcctcgatc agcttctgct ggttctcgtc cctgagcacg aactccagaa agctctccac
    18241 tgcctccatg tttctggtga acctgttcac cacaaagccc tgaaccgtgg ccagcggcct
    18301 gggaacctcc ccgttgcgtc ctttaggcag aggggcgatt ccgtagtcga cacacatgct
    18361 ctggtagatc gaggcattcc agaggccgta gagtatcatg gcggccctgc cgttggcgaa
    18421 ttcggccgat atatggcttt cggtaccgtt ttcgttggcc agcgtcaatc ccttcttctg
    18481 caactccttc atcctgttca cgtaatctag catgcaatcg ttgtccagac cgacgtcgta
    18541 ggggttgtaa tcgccgttgg gcgccttgcc gtagtagtag cccccgtggt cttttattat
    18601 ggggtagttg aaccacatat ccttggggtt gagcagtgta ccgtactgga ctatcttccc
    18661 gtcttcgttt ctaagagtga gagcctcggc gatcctgaaa aagtcgtccc aggtttctgg
    18721 cagctccgac tcgcttatga gatccctgtt gtatatgaga ccggaagtat ctagtgaata
    18781 acctataccg tagcatttgt tgttgtaggt gaaggcttcg aaggtcgcat cgagataacg
    18841 cttcctgaga tcgtcgtcta tctctaaagg cacgatcaac ccggaagcga ccatgttgcc
    18901 gatatccggg gcctgcatca ggattatgtc cggccgatcg tcgagctccg cattgttggc
    18961 cagatccctc ggtgcgtcga atatggagcg tcggcccact acttcgacct cgatcccggt
    19021 gagggaggtg aactccccgg cgatcgcggc catggcaaga ttttccggtt cgatatggct
    19081 gaaccagata cgtatggtcg aatcaccgcc gtccggagcc gcgaaaagcc tcgcgcccag
    19141 cagtagggcc acctgaacaa tgactacgaa cctgaaccct ctcacagatc tatccccccg
    19201 tacgcgatct tcagagcgtc ttgaactctt cggtaaccct caccgcctca tccactccct
    19261 ctaccgggag gcagatcgag acgtttcccc ttctcatcgc ctccatgttc gccactatct
    19321 cgtcgaatga atcacagtcg atggcgagac agacaccgcg cgaatcctcc accagtcgcc
    19381 ctatgttctc tatcggcctc ggctggttgg gatcggtggc tatccagagg ctggccacct
    19441 tctcgagcga acagatctcc ttcaccaggt aaagcgccct tgaatgacag tgcatatgac
    19501 cggtgccgaa gtgatcgatc accctctggg tgtgtggcgc gcagaacttt ctgtacagcc
    19561 tgggagatat catgcaggcg atatcttccg acatatatat gcatttatcg ataagccaca
    19621 tcccgtactt gctgcctcca aggtacctcc tggccagcgc gtaaacgtct tcggcgaatt
    19681 ttatcgtcgc atcggcgcag taatcgagga gttcgtgcaa ctcctcggga gcttcgtaga
    19741 aatcggtgta gatcgcctct cccctgaggg cggccgccag atctagcggt gagaagaagc
    19801 ccttcatgaa gggaataccg gcgccggagg agagtttcag tatctcctcg cagatattta
    19861 gaaagttgcc gtaccagaag gaagttccga tttcgggcag cctccggaaa tcccttatct
    19921 ctcccagaac cggtttggac cagctcgtat ccttttgaaa ctctatattt ccgtacacga
    19981 aggccgaata atctccgatc cccagaaccg gtgaaaccga agggatcatg ttgtcgtcga
    20041 cggctctgcg cttctcgaaa gattcgctca taagaccgca aagatctcgc gcgtacagat
    20101 ctatatcgtt cctgtaattg tagcgggtga ggtcgagcct ggaataatgc tcaacgtcgc
    20161 acttcgggag cgcccttatc gtaacgttac cccccctgtg gctcagcgcg tcttcataag
    20221 tctttcccat catcgctatc gttttgtcga tgtcgggctt ataacgatcc agttccctga
    20281 acaccgcatt cactccttat cactcatcga ccctcaaagg cagatcggcc ggcaactcta
    20341 ttgatttgaa aaacctctgg acgttgtcat agtactcccc gacatctatc ccgtcccaga
    20401 tgtaccctcc gttcctcaag gtggagagga gtataatatc cagaagcatc tggtcgatag
    20461 acatctccat cagcatgatg tttcgcgtgg ttatacctat ctcgtttatg ctggaattcc
    20521 cctcgcccat gaccatgaac tccctatcga acacgcctat tatcctgcgc cttggaaagt
    20581 tcatgttcgc gttcttcatc ttgtaggtga actggacgcc gaaatcgaag ggcatcttgc
    20641 agaaggagaa gatatatatc ttcactcccc tcccgtcggc ctctctcagt tcgttttcga
    20701 agagcgccgc ctcggaatcc cagagagaga gatagatctc ccgtttcgcc tcctttatgg
    20761 cgttcgcgat gaggtttagc atctccgcat ggttggagag gttcagtata tagttgtcgg
    20821 catccacctc gcgcgtgatc ttttccagag aactctcgag atcgtcgagt ttttccgtgt
    20881 aatcctttct gagagaggct atgagttttt tgtagctcac ggccgagtac agcttcgggt
    20941 taccctcgat cacgttgacg ctgcccttcc tctccagtct ctgtaagatg tcgtagacca
    21001 tggtccgggt tatcttggcc tttttagcga cctcgtaacc ggtcatggga ctgctcttgt
    21061 ggagtgcgat ataacatttg gcctcgttga gcgagaaacc cagttctcta agtatcttca
    21121 gctcgttcat aagccaccac caatcggaat cacttagtag taatagtcat tactactata
    21181 attgtagcat catggacgtg aatttcatcc ggccattacg gccctctgga gacggcctgg
    21241 aagaatgacg gagggatatt gcgaaatatc gttattaagc gagtccgtat ctcgagaaat
    21301 catgaagtca gatgatagaa aaaggagcgt ggtgggttgt gcgttggcaa aacacgagag
    21361 aaagagcgtc gagagttcgc tccgctcacg agaaggcgag aattctcaca tatcttcaat
    21421 ctctctcttt ttctctcttc tctctcctcc tctactcttt cgaaggacgg gtccatgctc
    21481 tgggacgacg gacgctggac ggatcttccc gcctcacaga cgcacatttt tttggaaggt
    21541 tgtctttctg agtatcgtcg agcctatcgg cttcaggaat actgtctttt cttcaccgga
    21601 gcttctatcc agaaactttc ctctcatgag gggcggatct atgaagtctc tcgaatgttt
    21661 atcgtttgac acggcgaggc tttcgagcgt cttttccgtt gcgcaaagtt cgaggaagga
    21721 acggtcttcc ggcaggaaga ggttgtcttc aaagccctta ccgtacagac gcccgggctt
    21781 ttctgccgat tcgatctcca tcgcgtaaag gagcggtccg taagaataat agacgtcgcc
    21841 cttcagatcg agtcgctctg ttattctgcc tgtgaactct atgagtatcc tgtcgtcttt
    21901 ccagtctttc gaaaccgcga tcgaatcaac acaatcctcg accttcgcac cgttggcttc
    21961 cactttcatc ttttcgaccc aggagggttt tttaagaagg atcgtaaaat tgctccgcac
    22021 ggcggtggtg accaggagtt cgatattcaa gtcgaagggg taagatgtaa tctgttcaag
    22081 ggcgatatcg attccgtcga tcgacgtctc gactctcgaa ggtccataca gcgccacgag
    22141 aagaccgtgc cgactcttca tgtacatcga gcctgtgaaa tagggaagta ccctgccggc
    22201 gttaggcgca cagcaaacgg ccacatcctg atgaacggga gagtatttat agcgcagatc
    22261 gttctcgctt cccattccgc cgacatgacc gttcatggcg tacgagttgt cgctcttgca
    22321 gtaagctatc tggcttttgc ggggattcac ggccccgtgc gccgcgttgt agtaaagcca
    22381 ttcggccctg tcggcccagc ggagatcgcc cgtcgtctcg agaagcgtga gataactgtg
    22441 gagcagctcc tggatcgagc agaattcgta gcccgtctcc gtagcatcgg cgcttcttcc
    22501 cccgatccat tcatcgccga tcggagcgcc agaaggggtc gtgacgaagt tcaatctgtg
    22561 caggtaagcc tctattgcgc ggttgtatcg cccgtccggc atgctggcta tgatcaacga
    22621 cctcaaacct tcgtaggtgt gaacgccgtg tcctttgaat ctgtaagcgt aatcgaggag
    22681 gttgtcctcc cttatatctt cctccgaaag acgggcccgg ttgtagaaat cgaccagccg
    22741 gccggcaaaa cggaggtact tcttatcccc ggtcaacccg aagagcccgt ggcagacgtc
    22801 ggtgaaggcc agtccgtgac tggcaccggc gtaggccttc tcgaggtcga agggatttct
    22861 ctccgaatag gccttcatcg taagatcgaa ggccttcatc actctctcga gcacttcact
    22921 ctctccggtg agttcgtaat aacccagggc ggcgcgaaga aaggtcgcct gtgcccagag
    22981 ttcgccgttc tctcctttga agtcgaaccg gagatctccg gcgtagatgc ctatgtaccc
    23041 gtcgtcgtcc tgcgccataa ggacccttcg caggtatttc ttcgccttat ctatatattc
    23101 gtcgttcccg accagatagg cgtgtcttat cagtccgtcc agccagttgg attgcgattc
    23161 gctgttccac cagaggtact ggacctccca ctcctgatcc tgggccacgg tcccgagatc
    23221 tttcgatttc accgcgccgg tcaacctgtc gcggccgtag atctcgtcgt cccttattat
    23281 ccccggaacc agatcgtcga gttcgccgac cagacccttc aggtttcgcg cggcctcttc
    23341 aaagagccag ccccggggcg ctatactacc gtagaggagt ctcttcaagc tcatcgctca
    23401 cctctcgggc agctccggtc tcaccacggt gtacttttcg aagagtttca cggcgaacca
    23461 gccgcagaaa acgcccagaa agatcccagc gaccacgtcg gagaagtagt tgaagtacac
    23521 gtatatcttc gaaagcgaaa cgagcgaggc gatcgcgaga accggtattc tcaacgactt
    23581 cacgctcttc catgtcatcg ccacataagc gaaggcgacc gtgacctgcg aggaagggaa
    23641 ggagtatgtc tcgggtagag ggaacataag gctgtaacct tccagcagga ggaagggtct
    23701 ggtcctggcg agaatcggtt tcaagatgac gaagctcagt agcagcgaga tcgccaggcc
    23761 ggccgaagta agatagccgg ttcttctgta cctcttatcg aagagcagga agacggtcag
    23821 gagaatccag accagcgagc ctctcgccag gaagagaaag aaagacatca acaaatccat
    23881 gaaagctcct tcgatcggcg agaccagcca gttcattaga gagagctcca gcccgttcaa
    23941 tccctgccac atagaaacct cccggcaaac aggttaaaat caggccaatt atacaacaga
    24001 aaaaggcggc ccggtgggcc gccttcgata attcaaggac tttgtattac ttcaatttgg
    24061 cctgcagagc agctatctgg acatcgctca gtccgatttt cgttttcaga tagttaagcg
    24121 caacctcttt gatgttggag tcatcgaccg gattaccgtc gttcatcgcg acgagtatat
    24181 cggctatgat cccctcgacg gcttcgtagg ctagcgttcc gggcgcgact ttgtagtagt
    24241 tctcgtaaga cttgacgtaa tcggcgtata tctcgccggt ctttgcatcc attatcgccc
    24301 cgagcagagc ccctacaatt ccggccctat ccttgccctc cacgcagtgc accaggtacg
    24361 gcggttcctt ctcgatcatg aaaagcaggc ccgacttgag cttctgggaa aaatcgtcgc
    24421 tgagcatatc gactcccatg ttcaggttga tcagattccc ggcctcgccg atcgatttgt
    24481 aatactggga gtaggcatag ctggccgcca gctcttcgtc ggagtcggag aggttgatca
    24541 cggtctttat gccggcagcc tcgacgagag cggccgcgta gggtgccctg ggatcgtcga
    24601 tggatgggtg ggaacatctg tagagtctgt ggggggcaat gtttccgatc gtgatctccc
    24661 tgaagttggc gaagacctcg tcgctcgcgt aatcgctacg caggtcagtt ctcaccaggt
    24721 ggcgtatctc gagctcttcc ttgtaagcgc ccttttcgac cagggccatt ctaatcattg
    24781 tgccggcttc gaggccgtaa gtctgggcga agtttccgta attgatggct agaaggacgt
    24841 gatcgccgga gtatctagca agcgcgcttc ccctgtccac atcgccgtag gtggtaacga
    24901 acgggactac tatggtggtc tccccgatcc tgatcgagac cgtatcgccg gcttctatgg
    24961 ccgcataaac tacagcttcc accggtatgt ccgtgtgcac gtttccgtac ttgtccactt
    25021 cctgcacgtg acctattatt cccgcgaata ccattgcaga gagaacaaca agcacaccga
    25081 tcaaaacgaa cttctttagc atctcgcacc tcctgcctgt tgatgaagtt gaactgtatc
    25141 tgagcaagac aattatatca tcatagaatt ctaacaaaaa aagcgaaccc ggttatcggg
    25201 tccgccacag aagagcgact ggaaatcaga aaataagtac agatcttatc acgagttctt
    25261 atcgatggcg tacagcgccg cgtaaagccc gctcataagc tggatctgca ccagtttctt
    25321 tatcggtatc actacatagt cgagctcgcc ttccagggcg tcgctcatct ctttcatctg
    25381 atcgtacact ttttccgcgg cctctttgaa ttcacactcg ccagtctttt cgaagttgag
    25441 gagcaggatc cttctcagca taccccacat aagcatcttg taag
//
LOCUS       DAOOLY010000004        23262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_2110_length_23262_cov_4.807774, whole genome shotgun sequence.
ACCESSION   DAOOLY010000004 DAOOLY010000000
VERSION     DAOOLY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 23262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23262
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_23262_cov_4.807774"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLD15_00415"
     CDS             complement(<1..730)
                     /locus_tag="PLD15_00415"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPB62386.1"
                     /translation="MYKEIGLSPMAGYTDGVMRELAMEWGADFAFSEMLSVEGVLRST
                     EKTEEVIPSGPCRIQLFGSDPGRMAKAALKLKDVATWFDINAGCPVRKVTRRGAGSAL
                     LKDPGKIGEMIKALKEVSCVPVSVKIRLGWESVESERIIPPIAEARPDAVFIHGRTVS
                     QGYSGSANWIEIDRIAGTLRREGILSYGSGDLFSPQAIAEALNNHSIDGVVVARGAIG
                     NPWIFKQARDLMELGAYEELGLSER"
     gene            complement(723..1295)
                     /gene="nadD"
                     /locus_tag="PLD15_00420"
     CDS             complement(723..1295)
                     /gene="nadD"
                     /locus_tag="PLD15_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPB62387.1"
                     /translation="MSSENRIGVFGGSFNPVHNGHIIIAIRALEELELDRLYIVPAYL
                     PPHKREINLAPYELRKKWLEKGFEGIGKIVISDYEKERGGISYSLFTMRYFSQKHSCR
                     PYLIAGEDSYRALESWYEYRMLLKESRLAVYPRSCNEKPLQLVEGAVLLKAPMIELSS
                     TEIRKRVRSKRSLLGMVPGSIIEEVEKTYV"
     gene            complement(1279..2598)
                     /gene="obgE"
                     /locus_tag="PLD15_00425"
     CDS             complement(1279..2598)
                     /gene="obgE"
                     /locus_tag="PLD15_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPB62388.1"
                     /translation="MLANDQESLVDTGRIYVKAGDGGNGAVSFRREKYIPYGGPDGGD
                     GGSGGHVFLRASNSRNTLYEFKHKRKFMAQDGENGGGSNMAGRKGRDLVILVPVGTIA
                     YDAETGETLADLNRPGETVVLARGGKGGRGNARFTSSTNQAPKASENGEPGEELYVRL
                     ELKILADVALIGFPNVGKSTLISVVSNARPKIANYHFTTLSPNLGVVMATEFLGYIVA
                     DVPGLIKGAHQGVGLGHSFLRHIERCKILVHLLDIAQTEERDFVQDYFDIRYELEFYR
                     AELLEKPEIVVGNKSDLLTPDMLNERVRLFKEKTGKEILPISAATSKGVDDLKRLVWN
                     VIGDSESFLNRLKVEEDLPLPVVEAIVYTAPEPENFKVEKDSRGRFVVFGPAVDFYFR
                     KIKAQKFRDRFIMDKLEKGGLTNKLKNAGIEEGDTVVIDDREYVFRE"
     gene            2665..2904
                     /locus_tag="PLD15_00430"
     CDS             2665..2904
                     /locus_tag="PLD15_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPB62389.1"
                     /translation="MDHIVNIGFESFVVKDRVLAVLPVQSSAVRRLKQLGMETGKVVN
                     LTFGKRTKALLITDSGHIIFSFLPPKRIIEKLFKN"
     gene            2925..3530
                     /gene="clpP"
                     /locus_tag="PLD15_00435"
     CDS             2925..3530
                     /gene="clpP"
                     /locus_tag="PLD15_00435"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPB62390.1"
                     /translation="MAPFVPYVVEDRGRGERIFDIFTKLLAERIVFLGWQIDDEISNI
                     VVAQLLFLESQDPDKDINLYINSPGGSITAGLAIYDTMQYIKPDVSTICIGMAASMGA
                     ILLTGGTKGKRYALPSSRIMIHQPFGGAEGVAKDIEIRAKEILYLRDELNKILAFHTG
                     QSIKKIEKDADRDYFMSATEAVDYGMIDKVIEPKNREKAKE"
     gene            3592..6060
                     /gene="mutS"
                     /locus_tag="PLD15_00440"
     CDS             3592..6060
                     /gene="mutS"
                     /locus_tag="PLD15_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPB62391.1"
                     /translation="MSEITPMMQQYLEVKNNYKDCIVLFRLGDFYETFFDDARLVADE
                     LQIVLTSRNGNPMAGVPYHAIDGYVKELISAGHKVAICEQTEDPAQAKGLLRREVTRV
                     MTPGTLMEDELLASQSNNYMLTVSEKKGTFAVILSDISTGEVVATTCESLPELLDIIS
                     GFEPAQILLKESLKTLKKDIMTVSSAFIEIFEDWNFALSSSLRYVKEFYSLASIDHLE
                     LDSSQLEALGALFKYLQITNLKPMKHLSLPRVVKKSETMQLDASTIENLNLLASQGKG
                     SLIAIMDENMTGMGKRKLKQWLLTPRTDVHKIERRLDTVQSLSEDPLLLEELREYLGC
                     IFDLERIVSRLASDRASPRDLQSLRNSLRILPLVKQLLMSNPSLRETGEGIILFSKEL
                     ELLERALQDEPSAFVGDGKVIREGFSRELDELRDLMENSVEKLKELERREKEATGIST
                     LKVKYNKVFGYFIELPKAQASKAPESYTRKQTLVNCERYITPELKSFEERVLSAGERI
                     DSLERSLFEDLCRETGNSVKDLQELSTVLAELDVLQSFASIARKYGYIRPEFASDGTT
                     RIEASRHPIVERFVGEFTPNDIAFGEEGRFFILTGPNMSGKSTYIRQIALISIMAQIG
                     SFVPASRAVLPVYDRIFTRIGARDELAGGKSTFLVEMMETATILSRATEDSLVILDEV
                     GRGTSTFDGISIAWSVSEYIYEAIGCNTVFATHFTELTELSQMYSGIRNKTVKIMETG
                     KGIVFLHRVVDGVASKSHGIDVAKLAGIPGVVLERAEEILRVISKQSALDKAVRVLTA
                     DDLVEIRRTKKGKMNRNQITLFDT"
     gene            6078..7157
                     /locus_tag="PLD15_00445"
     CDS             6078..7157
                     /locus_tag="PLD15_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPB62392.1"
                     /translation="MSRIEQFRSKLAEMGLEGFVLYNYESSNKSSSWYLSDFSGSFSV
                     LVVSPVGEYIITDSRYYTQASIETGFKLIPYKSGDELKDLISNILKNDGAGKVGFEED
                     TISHGLYKRVFENLGVELFPADVILKEMRMVKTPQEIEKIKSAVVVAEEALLETLNYI
                     RPGKSEEDIAAILEHEIRTRGGYLAFDTIVGSGPRSAVVHGKPSDKKLREGEFVLIDY
                     GARVDGYNSDITRTFSLGSPSEEMIKVYEVVYEAQKAAKEAARAGIIGSDLHEVAASI
                     IRQAGYGEYFGHGLGHSLGMDTHDGSGGASPSNKNPIPAGAVITIEPGIYLPEKFGVR
                     IEDDLYFGEDKTEVLTNLDRELKIL"
     gene            7179..7736
                     /gene="efp"
                     /locus_tag="PLD15_00450"
     CDS             7179..7736
                     /gene="efp"
                     /locus_tag="PLD15_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB62393.1"
                     /translation="MIEVGKLRKGMPLLIDGEIYIVLEANKHSMGRGDGIIRTRMKNL
                     KTALVRQFTFQASEKVEEASLSIRHVQYLYSEEDFFHFMDLETFEQYTIGPDEMGDGL
                     EYLKGNEEVDLQFHEERPIGVILPNTLVLQVTETAPAYKGDTVSGSGKPAVLETGLKV
                     TVPFFVENGEMIRVDTRTGEYIERA"
     gene            7757..8230
                     /locus_tag="PLD15_00455"
     CDS             7757..8230
                     /locus_tag="PLD15_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPB62394.1"
                     /translation="MDFEETDLGRIEISEAVIRDIAIHSYIEFLKFDPRENKARKEAK
                     STVDIDLDEKVDGTKTVKITVNTKIKYGVSIPSYARKMQEKLKNDVESFSGLKVEDVS
                     ISIEDVYEEPQKPAVIEEDEPEHEAALPQQESQAVESPVAEEEIEKEEEKKEKEV"
     gene            8238..8681
                     /gene="nusB"
                     /locus_tag="PLD15_00460"
     CDS             8238..8681
                     /gene="nusB"
                     /locus_tag="PLD15_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB62395.1"
                     /translation="MTGPNKSRRKMREIVFSAIYQFDFTEDAETSSAYLERELSFFAM
                     DMEMKLITRKYFENILRRREEIDGIIRKHLMNWTFDRLASTDRNVLRLGTYEIIYENA
                     VPIEVTLNEAIDIAKKYGSEQGGKFVNGVLDRIARECAPVAKKDL"
     gene            8695..10365
                     /locus_tag="PLD15_00465"
     CDS             8695..10365
                     /locus_tag="PLD15_00465"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPB62396.1"
                     /translation="MLTDLEIAQKAELKEISEIASSLSIPEKYIRRYGRNVAKISHHY
                     LEELKDRPDGKLVLVTAISPTSAGEGKTTTSVGLAMALNKIGKKTFVTLREPSVGPVM
                     GIKGGAAGGGYSQVLPMEEINLHFTGDFHAISQAHNLLSAAIDAHIKFDNKLGIDPTM
                     VFWPRTMDMNDRALREIIVGLGGKSNGYPREESFVITPASEVMAIICLSKDIDDLKER
                     LSRIVVARSKEGGFITAKDLKVHGAMAALLKDVLDPNLVQTLEGTPAFVHGGPFANIA
                     HGTNTIIATRTALKLSDYVITEAGFGADLGAEKFLDFVSPIAGFKPSSVVLVASIRAM
                     KLNGGASKKEVNQENLEALRGGFENLKVHYENLRKYGIPVLVALNEFPNDTEKERELF
                     DSLCAENTIPYEVSRVWAKGGDGGIEMAEKLVQLVEGGVNNYKPLVDLDLPIEEKIKK
                     LAKEIYRAGKVEFLPEAQAKLRLFKKQGLDRLPVIVAKTQYSITDDEKKLGAPSGYTF
                     NVRNLNLSAGAGFIVAVSGSIMLMPGLGEEFCAQQIDVDKNGKISGLF"
     gene            10376..11200
                     /locus_tag="PLD15_00470"
     CDS             10376..11200
                     /locus_tag="PLD15_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPB62397.1"
                     /translation="MILDGKSVAEKIYSEIRGKIEGLDSLPSLVLLCDEPDSSNKTYM
                     KSIVKQGEKLGINVVIENTGEDPIAQVHRMNSDPSIAGVMVMHPLKNADEKSVLAALD
                     PLKDLEGRTVSNLGGIVNDEEFFAPPTAEAVMEMLFQYEINPKGRDVTIVGRSNTVGK
                     PLALLLLKKGVDATVTVCHSRSRDITEKTFNADIVVSAVGIAKFLKREMVRPGSVVID
                     VGINFLDGKLVGDVDFDEVSQIAAAITPVPGGVGSVTTALFFKHYLKSLERMGRIA"
     gene            11200..13077
                     /gene="dxs"
                     /locus_tag="PLD15_00475"
     CDS             11200..13077
                     /gene="dxs"
                     /locus_tag="PLD15_00475"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPB62398.1"
                     /translation="MNESPLFRRLKDYTYPQLAMLAEEIRETITSVVATNTGHLASNL
                     GTVELTLALYRVFDPDRDIIIWDTGHQAYTHKLLTGRYGSFRSLRKKGGLSGFLKVSE
                     SPYDRFGAGHVGTALPAALGMEKSDELFKISRNIIVVAGDGAITSGMALEALNQMKDL
                     GSKIKVIMNDNGMSIGKNVGSLSSAMAEMRLNPFYREIKGDLKNTLETMKLGGLEQLL
                     SKIKSGLKATILGGNVFEDMGLNYIGPVGGHDIQEMETLFNAVKEIDEPFFVHVVTKK
                     GKGLEYAEKNPARFHSVGKIDPESGEKTFDSVEVSYSDVFGRTLTELASLDPSIVAIT
                     AAMPDGTGLSGFMEKFPARFYDLGITEQLCTTFAAGMAVSHSKPVFAVYSTFLQRAFD
                     QLAHDVALQDLPVLFAVDRAGIVGQDGPTHNGIFDIAFLSMLPNMRVLSPSSVQELSN
                     MLYTLLLKGMEHPTAIRYPREAEVISLDEVLSSMKEIALWKWEEIMHGEEGAILATGS
                     MVSRSYEVALKYGYALYNCRSIKPLDESMLEYVFGKYDTIVCVEEGIKRGGFGSGIAL
                     KAIEHGYAGKIDLVAIDDCFSPHGTRDEILSELGLDTRGIEKTISRLRGEKNASDYGI
                     R"
     gene            13052..13399
                     /locus_tag="PLD15_00480"
     CDS             13052..13399
                     /locus_tag="PLD15_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPB62399.1"
                     /translation="MQVTTEFGKIDISLQAICSIVKKVVSESYGPVNVGTPQTGFLAK
                     LFGTAEDTRKGIKVTEEIDGTLEIDVDLILEYGVRIPTVVENIQENVFHKLKELTEAT
                     NIKVNIHVVGLQD"
     gene            13415..15079
                     /locus_tag="PLD15_00485"
     CDS             13415..15079
                     /locus_tag="PLD15_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPB62400.1"
                     /translation="MKRINGKFFVAAFRKAAERLLANKDEINALNVFPVPDGDTGSNM
                     SAAMIEACEYLDRLKKDDLVSVLESVKTGMLMGARGNSGVILSQIFRGFAEGIGNRKF
                     VSTKAFTEGLIKAKEIAYRSVMKPVEGTMLTVMKVVAETANEHYTELEDFEEYFDEMV
                     KTAFDTVEKTPSLLSKLKEAGVVDSGAKGLAYIFEGFQLAIKGELEVDNLIQQMPQAI
                     GSSTERIVEIVREELKYTYCTELIVNLHDSENPEETYDGLKAFLEEMGDSIVMVNQDE
                     FIKVHVHTDHPGDIIEKFLHVGFLQKVKIDNMKVQHEHVVDIQTKASEPFGKGKKHGI
                     VVISPGDGLADVLKSLGVDYTVRGGQTMNPSLKDLYEAISRMEPDKVVVLPNNPNIIL
                     TAKEAANAVHDENPNKEVYIIPTRTVQEGISAMTVYNDDSDTQELIEEMTQAADAVTP
                     ISITYAVRDSSMKGKKIKKGEYIAIGKDGLLGSGKKLDKLVQDSIKAVIGKESNREVV
                     TVFYGSDVSEETAAKLAETLSSNFSDLEFEIHSGGQPYYYYLISIE"
     gene            15094..15948
                     /locus_tag="PLD15_00490"
     CDS             15094..15948
                     /locus_tag="PLD15_00490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPB62401.1"
                     /translation="MYLQNVIANLNNYWSSMGCIIDQPYDVEMGAGTFHPSTFLRSLG
                     KKPWKVAFIQPSRRPTDGRYGENPMRVQRYFQYQVIIKPNPENSQELYLGSLEALGIN
                     PVEHDIRFVEDNWESPTLGAWGVGWEVWLDGMEVTQFTYFQQVGGIDVDLVSLEITYG
                     LERITMYLQKKENIFDIDWNGEFKYGDVFLENEKEFSAYNFDVADTAKLFEFYRMYRD
                     EFDLCMKNGLIRPSYDYMIKCSHAFNLLDARNAISVSQRQSYIKSIREMARSVAQAYV
                     DREGESDA"
     gene            15941..17977
                     /gene="glyS"
                     /locus_tag="PLD15_00495"
     CDS             15941..17977
                     /gene="glyS"
                     /locus_tag="PLD15_00495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPB62402.1"
                     /translation="MHKKKALLEIGVEELPSSEVLAIRNQLREKIEKSLQASRLGYDG
                     LRIFTASRRFGLYIDGLSPKQPDYLEEKKGPSEKIAYKDGEPSRALLGFLKSNDAGSE
                     DITVKDGYVYIERKIPGKSSLEILPGIFRQAVLSLEFKKPMRWGDGTYRFVRPVKWVV
                     SMIDNTVLEMELFGKKASDTTKGHRFYFDEIRVSPENYFEKLREALVIADGSERERRV
                     LSEIEKTGELLQGNIPVDGELLEEVVSLTEYPTAVVGRFMEKYLALPPEVIIVTIKHH
                     QRTFPVYREGVLTDNFVAFQDGPGDSLGNIRLGYEDVINARLEDAFFYYEKDLEKPLE
                     SYVPGLEEILFQKGLGSFMDKSKRTAALAIKIGKLLKAKSGELESIERTALLGKADQV
                     TRVVQEFPEVQGVMGRIYAEKSGESEEVALGIEEHYQDQKVPSTLSGAVAGVADRIDT
                     VVGNFTIGNIPSASKDPYALRRKMAFIFRTMNYLGWKIDLKELLKESTGILGGQAFDT
                     LQSVASFCRSRYEAYLLEQGFSINVTRAVSDWWRFPYLGERAAKAITEYVEKAEFTDL
                     LIAYQRVHNISKNHDCDAFEGSKFVEQAERDLLNEYLECFESVSSALEKDDFEKALQL
                     LTGLKSSIDRYFDDVFVMDNQEDIRLNRLGFLKSLDGLFLKVGDLSLLLEEERK"
     gene            17974..18882
                     /gene="era"
                     /locus_tag="PLD15_00500"
     CDS             17974..18882
                     /gene="era"
                     /locus_tag="PLD15_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPB62403.1"
                     /translation="MSEIFKSGIVALVGKPNVGKSSLINRIIGEKIAIVSDKPQTTRN
                     RIGGIYTTDQGQIVFYDTPGIHKPLHKLGQYILKVATSSLLGVDLLLVMVDPTDGLRE
                     SDRLVATHVNGSHVPVFLVINKIDAYGDRKLLENFSENASRLFENVLERFYISAEKGT
                     GVDSLIEAIFKFLPAGKMLFPEDYITDRSSRFMASEIVREKVLQHTRQEIPHSVGVSV
                     HEFKDEGDLLRIRADIVVERNSQKPIILGKGGSMIKLIGSDARRDMEYIFDQKVFLDL
                     FVKVREKWRDKDSMIQEFTNLKDELS"
     gene            18899..20206
                     /locus_tag="PLD15_00505"
     CDS             18899..20206
                     /locus_tag="PLD15_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62404.1"
                     /translation="MKKSRARIVLFLMLFMMVFLNADQMVMSPNIGEIEAEFGITKAD
                     IGLIQGSFTIVGALVSLVWGFLADKYNRKLLLLLSVLVGEIPCFLSAFVQSFPQLFIT
                     RALTGIGVGALFPVVFSFAGDTFKESHRPKVNAFLSTAISLGAIVGMVIAGFTGSTLG
                     WRIPFIIVSLPNIFLALAFYFLAEEPRRGAAEVAVGELVDKGYSYAGKVKLSDYLNLF
                     RVRTNLVLFIQGILGTIPWGAIPYYLVNYFETYKNLSKELATLIFMFFGVGNVLGIFL
                     GGLIGGLLYKRKPSYMPLFSGGMTIAGTFVALGALNFPPVEGTGGFLMLGLLGMVAAA
                     TASMTGPNMKTMLMNVNEPESRGRIFSVFNLTDSLGTGFGQFFAGTLATAVGSLGVAM
                     NASALFWLPCGLVLLVAALTFPRDITRLHTRMQEVAKTMESVK"
     gene            complement(20369..21601)
                     /locus_tag="PLD15_00510"
     CDS             complement(20369..21601)
                     /locus_tag="PLD15_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB62405.1"
                     /translation="MRNILVIGAGPAGLFTAISCTTEGRNVTVLDKNDSPGKKLLMTG
                     SGQCNLTNSEAPKAMLKHYFGIEAQRFLKPALMAFDSSRLIEFFQKRGLKMTVRDDGK
                     VFPVTLSARDVLEVLLAECEKQGIVIQCNCRVASVDRDDDGTFLVKTPGGDLQANYLA
                     ITTGGKSYPYTGSSGDGYLFAQKMGHTTQEPRPALAAVYIDDFPFSDLSGISIKRAEV
                     SLWRNGGKIAERREDLLLTHTGLSGPSILHLSREILPDDTLLINLAGWKREDADRLLL
                     EKLTANGKKNVKNILADLDLPERLTEKLVELSGAAARTGAEIKKQERKELERLLTGLE
                     MKVSRTGDFLTAMMTRGGISILEVNSRSMESRLVPSLFFAGEVLDFDGETGGYNLQAA
                     FSTGYLAGNSIRVKSERP"
     gene            complement(21661..23154)
                     /locus_tag="PLD15_00515"
     CDS             complement(21661..23154)
                     /locus_tag="PLD15_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HPB62406.1"
                     /translation="MRRISRSSSVAVGTLAILTFSIISKGMGFFREMLIAGLFGTSGN
                     LDAVFIAMTPATTLSSIVAGALASIFVPIYHSIRNEDPLRSKKYAGAVFLAGSAVFLS
                     MGIVFLLIPDLVIRLFAPGFSEETIAYAARKLRFLSIYPLIGGLENLLGTLLKASRRF
                     IQFGISQLIFNVVAIPVILFTSPFLSEASYILAWIVGNALTVLLYALQSRDLFVLKFD
                     RGTRIVETLTLSLPLVLSGSLSLINGMVDKAFVSLLPPGRVSSLQYAHTLLGLITFVV
                     TAFLMTSYTELSEFIVSGEREKTRERLRKTVTSSLNIALPLAVWVISMAEPLVRFIYQ
                     RGEFDADSTRLVSMALIGYGALIVISPVSYTCSSYFIARRKVRFITLISFISIMLNVL
                     FDWLLLEPLGHAGIAASTSLVSLNSTVIYNLLIRKEGMNFMPFRRIMLLLTCAVSLYL
                     FVFALKYFSTTLLWLLLGNVIFFAFFLFSARSEIVAGLSRVRSMKRS"
BASE COUNT         6072 a         5744 c         6196 g         5250 t
ORIGIN      
        1 gtctctcgct caaaccgagc tcctcgtaag caccgagctc catcaaatct ctggcctgtt
       61 tgaaaatcca gggattgcct atcgcacctc tggcaactac aacgccgtct atcgagtgat
      121 tgttgagagc ctccgcaatg gcctggggtg aaaacaggtc gcccgaaccg taggaaagga
      181 ttccttcccg acggagcgtt ccagcgatcc tatctatctc tatccagttg gccgaaccgg
      241 aatacccctg cgaaacggtt cttccgtgaa taaaaaccgc gtcgggtctt gcttccgcga
      301 tgggcgggat gattctctcg ctctcgacgc tctcccagcc gagccggatc tttacagata
      361 cgggtacgca agacacttcc ttcagggctt ttatcatctc tcctatcttg ccgggatctt
      421 tcagaagggc actccccgca ccacgcctgg ttacctttct aaccgggcag ccggcgttga
      481 tatcgaacca cgtggccacg tccttcaact tcagagcggc cttcgccatt ctgcccggat
      541 cggaaccgaa gagctgtatc cggcaggggc cggaggggat cacttcttcg gtcttttctg
      601 tagatctcag cacgccctcc acgctgagca tctcgctgaa ggcgaaatcg gcgccccact
      661 ccatggccag ctcccgcatt acgccgtcag tgtagccggc catgggggac agtcctattt
      721 ctttatacat aagtcttttc gacctcttcg attatcgatc cgggaaccat tcccagcaaa
      781 gatctcttcg atcttaccct cttccttatc tcggtagatg aaagctctat catcggcgcc
      841 ttgagcaaca ccgctccttc cacaagctgc aacggctttt cgttgcacga acgtggatat
      901 accgccagcc gggactcttt cagaagcatt ctgtactcgt accagctttc cagggcgcga
      961 taactgtcct caccggcgat caggtaaggt ctgcaagagt gtttctgcga gaagtacctc
     1021 atcgtgaaga gcgaatagga gatacctccc ctctcttttt cgtaatctga gatcactatc
     1081 tttccgatcc cttcgaagcc tttttccagc catttttttc tgagttcgta cggtgcaagg
     1141 tttatctctc tcttgtgtgg aggcagatag gcggggacga tgtagagcct gtccagttcc
     1201 agctcttcca gagccctgat cgcaatgatg atgtgaccgt tgtggaccgg gttgaacgac
     1261 ccgccgaaca ctccaatcct attctctgaa gacatattct ctatcatcta tcacgacggt
     1321 atcgccctct tcgataccgg cattttttaa tttattcgtg agtccgccct tttcgagctt
     1381 gtccattatg aatctatccc tgaacttctg cgccttgatt ttcctgaagt agaaatccac
     1441 tgccggtccg aaaactacga accggcctct ggaatccttc tcgactttga agttctccgg
     1501 ttcgggagcc gtgtacacga tagcttccac taccggcaag gggagatctt cctcgacttt
     1561 cagcctgttc aggaaggact cgctatcgcc gataacgttc catacgagtc ttttgagatc
     1621 atcgactccc ttcgaagtag cggccgagat aggcaggatc tccttgccgg tcttttcttt
     1681 gaagagtctc acccgctcgt tcaacatgtc gggggtgaga agatcgcttt tattgccgac
     1741 cactatctcc ggcttttcca ggagctccgc tctgtaaaac tccagttcat aacggatgtc
     1801 gaagtaatcc tgcacgaaat ccctctcctc ggtctgcgca atatccagca gatggacgag
     1861 tatcttgcat ctctctatgt gcctcagaaa actgtgtccg agacctactc cctggtgcgc
     1921 gcctttgatc agaccgggca cgtcggccac gatgtatccc agaaactccg tcgccattac
     1981 gactcccaga ttgggagaga gcgttgtgaa gtggtagttg gctatctttg gtcttgcgtt
     2041 tgacacgacc gatatcaggg tcgatttgcc cacgttggga aaaccgatga gtgcaacgtc
     2101 ggctagaatc ttcaattcga gccgcacata cagctcctcg ccgggctcgc cgttctcgga
     2161 ggctttggga gcctggttgg tggaagaggt aaaacgtgcg ttccccctgc ccccttttcc
     2221 gcccctggcg agaacgaccg tctcgccggg cctgttcaga tcggccagcg tctcgccggt
     2281 ttcggcgtcg taagcgatag ttcccacagg cactagaata acgaggtccc tgcccttcct
     2341 gccggccatg tttgaccccc cgccattttc tccgtcctgc gccatgaatt tccttttgtg
     2401 tttgaactcg taaagcgtgt ttctgctgtt acttgctctc aagaagacgt gtcctccgct
     2461 cccgccgtcg ccgccgtcgg gtccgccgta gggtatgtat ttctctcttc tgaaggagac
     2521 ggcaccgttg ccgccgtcgc cggccttgac gtaaattcgc cccgtatcaa cgaggctttc
     2581 ctgatcgttt gcgagcatcc cagcacctcc gtgggtcttc tattatgtta taataacatc
     2641 gttgaaggaa ggaggaaagt cgaagtggac cacattgtca atattggttt cgaatccttt
     2701 gtcgtgaaag acagggtgct tgctgttctt ccggttcaaa gctcggctgt gagaaggttg
     2761 aagcagctcg gcatggaaac gggaaaggtc gtcaacctga cgttcggtaa gagaaccaag
     2821 gcacttctca tcaccgatag tggtcacatc atcttctcgt tccttccacc caaaagaatc
     2881 atagaaaaac ttttcaagaa ctaggaggaa ttatggatat aaacatggcg cctttcgtac
     2941 cctacgtagt cgaagataga ggaaggggag agaggatatt cgatattttt accaagcttc
     3001 tcgcggagag gatagtcttt ctcggctggc agatagacga cgagatctcc aacatcgttg
     3061 tagcccagct cctattcctt gagtcgcagg acccggataa agacatcaat ctttatataa
     3121 acagccctgg tggatcgatc actgccggcc tggcgatcta cgacaccatg caatacataa
     3181 aacccgacgt ctcgacgatt tgtataggta tggccgcttc gatgggagcg attctcctca
     3241 ccggcggtac gaagggaaag aggtatgccc tgcccagttc tagaataatg atccatcagc
     3301 ctttcggcgg ggccgaaggg gtagcgaagg atatcgagat cagggccaaa gagatccttt
     3361 acctgaggga tgagctcaac aaaatcctgg ccttccacac gggtcagtcg ataaagaaga
     3421 tagagaaaga cgccgaccgc gattacttca tgagcgcgac agaggcagtc gattacggga
     3481 tgatagacaa ggtgatagaa cccaagaata gagagaaagc caaagagtga cgaatttttc
     3541 tcttatggcc ggcgcaaagc gccggctttt gacgtatctg gggttggtag tgtgtctgag
     3601 ataacaccga tgatgcagca gtatctcgag gtcaaaaaca attacaaaga ttgcatagtc
     3661 ctctttcgtc ttggagactt ctacgagacg ttcttcgacg atgccagact ggtcgccgat
     3721 gagcttcaga tagtgctcac ttcgcgaaac ggcaatccca tggccggcgt cccctaccac
     3781 gcgatcgacg gttacgtgaa ggagttgatc tccgccggcc ataaagtcgc catctgcgaa
     3841 cagaccgaag acccggcgca ggctaaggga ttgttgagga gagaggtaac ccgcgtcatg
     3901 actcctggaa ccctcatgga agacgagctt ctcgccagcc agtccaacaa ttatatgttg
     3961 accgtgtccg aaaagaaggg cacgtttgcg gttatcctga gcgatatttc taccggggaa
     4021 gtggtcgcta ctacctgcga aagccttccg gaactcctgg atataatatc cggtttcgaa
     4081 ccggcccaga tacttctcaa ggagagtctg aaaaccttga aaaaggatat catgacggtt
     4141 tccagtgcct tcatagagat cttcgaagac tggaacttcg cgctctcctc cagtctcagg
     4201 tatgtaaaag aattctattc tctggcctcc atagatcacc tggagctcga ttcatcacag
     4261 ctggaggctc tcggagcact cttcaagtac cttcagatca ccaacctcaa accgatgaaa
     4321 catctatcgc tgcccagagt tgtcaaaaaa tccgaaacca tgcagctgga cgcctccacg
     4381 atagaaaatc tcaaccttct ggctagccag ggaaagggtt cgctcatagc gataatggac
     4441 gaaaacatga ccggtatggg aaagaggaag ctgaagcagt ggcttttgac accccgtacc
     4501 gatgttcata aaatagagcg gaggctcgat accgttcaaa gcctttcgga ggatccgctc
     4561 cttctggaag agctcaggga gtatctcggc tgtatcttcg atctggagag aatagtttcc
     4621 cgtctggcca gtgaccgggc ctctcccagg gatctccagt ctttgagaaa ctccctcaga
     4681 atccttcccc tcgtaaagca gcttctcatg agcaaccctt ctctacgtga aacgggagag
     4741 gggataattc tcttctcgaa agaactcgag ttactggaaa gggcgttgca ggacgaaccg
     4801 tccgcgttcg tcggcgatgg caaggtgata agggaaggtt tcagccggga actcgacgag
     4861 ctgcgggatc tcatggagaa ttccgtcgag aagctgaagg agctggaacg gagagagaag
     4921 gaagcgactg gaataagcac tctcaaggtc aagtacaaca aggtattcgg ttacttcata
     4981 gagctgccca aggcccaggc ttcgaaggct ccggaaagct acaccaggaa gcagaccctc
     5041 gtgaactgcg agaggtatat aacccccgaa ctgaagtctt ttgaagaaag ggttctcagc
     5101 gccggcgaga gaatagattc gctggaaaga tcgctgttcg aagatctctg tcgcgagacg
     5161 ggaaactcgg tgaaggatct gcaggagctt tcaacagtgt tagcggagct ggatgtcctt
     5221 caatctttcg cctcgatagc cagaaagtac gggtacatca gacccgagtt tgcatcggac
     5281 ggtaccacca gaatcgaagc ctccagacac ccgatagtgg agaggttcgt gggcgagttc
     5341 acaccaaacg atatagcctt cggcgaggaa gggcgtttct tcatactcac cggtcccaat
     5401 atgagcggga agtccacata tataaggcag atagccctca tctccataat ggcccagatc
     5461 ggttctttcg ttccggcctc gagggcggtt ttgcctgttt acgaccggat cttcacaagg
     5521 atcggtgcca gggacgagct ggccggcgga aaatctacct tccttgtgga gatgatggaa
     5581 acggccacta tcctttcaag agccaccgag gacagtctgg taatactgga cgaagtggga
     5641 aggggaacga gcactttcga cggcatttcg atcgcctggt cggtctcgga atacatatat
     5701 gaagccatcg ggtgcaacac ggtcttcgcg actcatttca ccgaattgac ggaactctct
     5761 caaatgtact caggaataag aaataaaaca gttaaaatta tggagaccgg aaagggtata
     5821 gtatttctgc acagggtggt tgatggtgtg gccagcaaat cgcacggtat agatgtcgcg
     5881 aaacttgcgg gcatccccgg tgtagttctg gagagggccg aagagatact cagggttatc
     5941 tccaaacagt cggcgctaga taaggccgtt cgagtgctga cagccgacga tcttgtcgag
     6001 atacgcagga caaagaaagg caagatgaac cgcaatcaga taacgttgtt tgacacatag
     6061 atcaatggag gttgagcatg tcgagaattg aacaattcag aagcaaactg gccgaaatgg
     6121 gactggaagg ttttgtcctt tacaactacg aatcgagtaa caagtcgagt tcctggtacc
     6181 tttcggattt ttccggttct ttttctgtac ttgtcgtctc cccggtaggt gagtatatca
     6241 tcaccgactc cagatattac acccaggctt cgatcgagac cggtttcaaa ttgataccct
     6301 acaagagcgg cgatgaactc aaagacttga tttcaaacat actcaagaac gatggtgccg
     6361 gaaaggtcgg cttcgaagag gacacgatca gtcacggtct ctacaagaga gtcttcgaga
     6421 atctcggcgt tgagcttttt ccagccgacg tgattttgaa ggaaatgcgt atggttaaga
     6481 cgccgcagga gatcgagaaa ataaaaagtg ccgtggtagt ggccgaagag gctctcctgg
     6541 aaactctcaa ctatataaga ccgggaaaga gcgaagagga catagcggcc attctcgagc
     6601 acgagataag gacacgtggc ggttatctag ccttcgacac tatagtcggc agcggtccga
     6661 gaagcgcggt ggtacacggt aagccttcag acaaaaaact ccgcgaaggc gagttcgtgc
     6721 tcatagatta cggcgcgaga gttgacggtt ataactcgga tatcaccaga accttttcgc
     6781 tgggaagtcc cagcgaggag atgataaaag tttacgaagt cgtctatgag gcccagaagg
     6841 cggcaaaaga agcggcacgc gccggaatta tcggcagcga cctgcacgaa gtggccgctt
     6901 cgataataag gcaggccggg tacggcgagt acttcgggca cggtctgggt cacagtctgg
     6961 gaatggatac ccacgacgga tccggtggcg cgagcccttc gaacaagaat cccattcccg
     7021 caggggccgt gatcaccatc gagccgggta tatatttgcc ggaaaagttc ggggtcagaa
     7081 tcgaggacga cctctatttc ggagaagata aaaccgaggt gcttacgaac ctcgatagag
     7141 aactcaagat actgtaacga tccggaggtg gcttctacat gatagaagtt ggcaagttaa
     7201 gaaagggaat gcctctgctt atcgatggag agatttacat cgttctggag gcgaataaac
     7261 actccatggg acggggagat ggaataataa ggacacgaat gaagaacctc aagaccgcgc
     7321 tggtcaggca gttcaccttt caggccagtg aaaaggtcga ggaggcttcg ctttcgataa
     7381 ggcacgtcca gtacctttat tccgaagagg attttttcca tttcatggat cttgaaacgt
     7441 tcgagcagta cacgatcggt cccgacgaaa tgggagacgg cctcgaatat ttgaagggaa
     7501 atgaagaggt agatcttcaa ttccacgagg aaaggccgat cggagtgatc ctacccaaca
     7561 ccttagtact ccaggtcacg gagacggcac cggcctacaa gggcgacaca gtttccggaa
     7621 gcggtaaacc ggcagttctg gagaccggtt tgaaagtgac agtaccgttc ttcgttgaga
     7681 acggcgaaat gatcagagtc gatactagaa ccggcgaata catcgaaaga gcctgataag
     7741 gaaaggaggg agaagaatgg atttcgaaga gacggatctt ggaaggatcg agatctctga
     7801 agccgtgatc cgggatatag cgattcactc ctacattgaa ttcttgaagt tcgatcccag
     7861 agaaaacaaa gccaggaagg aagccaaatc aaccgtcgat atcgatcttg acgaaaaagt
     7921 ggacggaacg aaaaccgtta agatcaccgt gaatacgaag atcaaatatg gggtctctat
     7981 tccttcttat gctagaaaga tgcaggagaa gcttaagaac gacgtagaga gtttcagtgg
     8041 tctgaaagtg gaggatgtct cgataagtat cgaggatgtc tatgaagagc cccagaagcc
     8101 tgccgttatc gaagaggacg agcccgagca cgaggcggcc cttcctcagc aagagagcca
     8161 ggccgttgaa agccctgttg ccgaagaaga gatagagaag gaagaggaaa agaaagagaa
     8221 agaggtataa ctccacaatg acgggtccaa acaaaagccg gcggaagatg cgcgaaattg
     8281 tcttcagcgc catataccag ttcgacttta ctgaagatgc agagacttct tctgcctatc
     8341 ttgagaggga actgagcttt tttgccatgg acatggagat gaagctcata acccgaaagt
     8401 atttcgaaaa tatacttcga agaagggagg agatcgacgg gataataaga aaacatctga
     8461 tgaactggac cttcgacagg ctcgcctcga ccgacagaaa cgtattgagg ctcggcactt
     8521 atgaaatcat ctatgaaaac gcggtgccga ttgaagtgac gctgaacgaa gcgatagata
     8581 ttgcaaagaa gtacggttcg gaacagggag gaaagttcgt caacggagtt cttgatagga
     8641 ttgccaggga atgtgcacct gtcgcaaaaa aagacttata atgggagggt cagtatgtta
     8701 accgatctag aaatagctca aaaagcggaa ctcaaagaaa tcagcgaaat agcttcctcg
     8761 ttgtcgatac ctgaaaaata cataaggcgg tatggaagaa acgtagccaa aatatcccac
     8821 cactatcttg aagagcttaa agatagaccc gatggaaagc tcgtactcgt tactgccata
     8881 agtcccacca gcgcgggcga gggaaaaacg accacctcgg tagggttggc gatggcgctc
     8941 aacaagatag gaaaaaagac attcgtcact ctgagagaac cctctgtggg ccctgtgatg
     9001 ggtatcaaag gtggtgccgc aggaggaggt tattcgcagg tccttccaat ggaagagata
     9061 aaccttcact tcaccggaga tttccacgct atttctcagg cccacaatct actttcagct
     9121 gcaatcgacg cgcatataaa attcgacaac aaactcggca tcgatcccac tatggttttc
     9181 tggccgagga cgatggacat gaacgaccgc gccctcagag agataattgt cggactcggc
     9241 ggaaagagca acggctaccc gagagaggag tctttcgtca taactccggc ttccgaagtg
     9301 atggcgataa tctgcctttc caaagatatc gatgacctca aggagcgcct gtctagaata
     9361 gtcgtggcga gatcgaagga aggcggtttc atcaccgcaa aggatcttaa agttcacggg
     9421 gccatggccg ctctgttgaa agacgtgcta gatcccaacc tcgtccagac cctcgagggg
     9481 actccggcct tcgttcatgg cggccccttc gccaacatag cccacgggac gaacaccata
     9541 atcgccacca ggacggcttt gaaactctcc gattacgtca taacagaggc cggcttcggc
     9601 gccgatctgg gagcggagaa gttccttgat ttcgtctcgc ccatcgcggg attcaaaccg
     9661 tcatcggtcg ttctcgtagc ctctattcgc gccatgaaac taaacggcgg cgcctcgaaa
     9721 aaagaagtga accaggagaa tctcgaagcg ttgaggggcg gttttgaaaa tctcaaagtc
     9781 cactacgaaa acctgagaaa gtacggaatc cccgttctgg tagcgttgaa cgaattcccc
     9841 aacgatacgg aaaaggaaag ggaactcttc gactccctgt gcgctgagaa cacaattcct
     9901 tacgaagttt ccagagtgtg ggcaaagggc ggagacggtg gaatcgagat ggcagaaaag
     9961 cttgttcagc tcgttgaggg cggcgtcaac aattacaaac cgctcgtcga tctagacctt
    10021 cccatcgaag agaagataaa gaaactggcc aaggagatat acagggccgg aaaggttgag
    10081 tttttaccgg aggctcaggc caagctaagg ttgttcaaga agcagggact tgacagatta
    10141 ccggttatcg ttgccaagac acagtactcc ataaccgatg acgagaagaa gctgggagct
    10201 ccatccggtt ataccttcaa cgttagaaac ttgaacctct ccgcgggagc gggcttcata
    10261 gtagccgttt cggggagcat aatgttgatg ccgggccttg gagaggaatt ctgcgcccag
    10321 caaatagatg ttgataaaaa tggcaagata agcggactat tttgaggtgg tttccatgat
    10381 acttgacgga aagagtgtcg cagagaagat atactccgaa ataaggggaa agatcgaagg
    10441 cctggattcc ctgccttcgc tggttctcct ctgtgatgag cccgatagtt cgaacaagac
    10501 ctacatgaaa tccatagtga aacagggtga aaagcttgga atcaatgtag tgattgagaa
    10561 taccggagag gatcccatcg cccaggtaca caggatgaac tccgatccct ccatagccgg
    10621 cgtcatggta atgcatccac tcaaaaacgc cgacgaaaaa tccgtgcttg ccgccctcga
    10681 tcctctgaag gatctcgagg ggcgtaccgt ctctaatctc gggggaattg tcaacgacga
    10741 agaatttttc gctcctccga ccgctgaagc ggtcatggag atgctttttc agtacgaaat
    10801 caatcctaaa ggacgcgatg tcacgatagt gggacgctcg aacacggtgg gcaaacctct
    10861 tgctttgctt ttgctgaaga agggcgtcga cgcgactgtg acggtctgtc actccaggag
    10921 tagagatata accgagaaga cgttcaacgc cgacattgtc gtgagcgccg ttggaatagc
    10981 gaagtttctc aagagagaaa tggtgaggcc cggttctgta gtgatagatg tcggaatcaa
    11041 tttcctggat gggaaactgg tcggtgacgt agatttcgac gaagtcagcc agatcgcagc
    11101 cgccataacc cccgtgccgg gtggtgtggg aagtgtaacc acggctttgt tctttaagca
    11161 ttatttgaaa tcgcttgaac ggatggggag aattgcctga tgaatgaaag ccctcttttc
    11221 aggagactga aggattatac atatccgcag cttgccatgc tagccgaaga gataagagag
    11281 acaataacat cggttgtggc aacaaacacc ggccatctcg cctcaaatct cgggacggtg
    11341 gagctcacgc tggcacttta cagggtcttc gatccagata gagacataat aatctgggat
    11401 acgggacacc aggcctacac tcacaagtta ctcaccgggc ggtacggttc cttcaggtct
    11461 ctcagaaaaa agggtggttt gagcggcttt ttaaaagtat cggaaagtcc ttacgatcgt
    11521 tttggcgccg gtcacgtggg aacggcactc cccgcggcac tcggtatgga gaagtccgac
    11581 gagcttttca aaatctccag aaatataatc gtagtggccg gtgacggcgc cattacttcc
    11641 ggaatggctc tggaagcgtt gaaccagatg aaggatctcg gctccaaaat caaagtcata
    11701 atgaacgata atggcatgag cataggaaag aacgtaggca gtctttcatc ggcgatggcc
    11761 gagatgagac tcaacccctt ctacagagag atcaagggag atctgaaaaa cacccttgaa
    11821 acgatgaaac tcggcggcct ggagcaatta ctttcaaaga taaagagcgg tctcaaggcc
    11881 actattctcg gcggaaacgt tttcgaagat atgggactga actacatcgg accggtcgga
    11941 ggacacgata ttcaggaaat ggaaacgctt ttcaacgcgg taaaggagat cgacgaaccc
    12001 ttcttcgtgc acgtagttac gaaaaaggga aagggactcg agtatgccga aaagaatccg
    12061 gcacgttttc acagcgttgg aaagatagac cccgagagcg gtgaaaagac cttcgactcg
    12121 gtggaggtat cgtacagcga cgttttcggc agaacgctca cagagctggc ttctctggac
    12181 ccttcgatcg tagccattac ggctgccatg cccgacggaa cgggactatc tggtttcatg
    12241 gagaagttcc ccgcgcgttt ttacgatctg gggataaccg aacagttatg cacgaccttc
    12301 gcggctggaa tggcggtctc gcacagcaaa cctgtcttcg cggtatattc tacctttctt
    12361 cagagagcct tcgaccagct tgcgcacgat gtggctcttc aggatctgcc tgtgcttttc
    12421 gcggtcgatc gggccgggat agttggtcag gacggtccga cccacaacgg tattttcgat
    12481 atagcttttc tctccatgct tccgaacatg agggttcttt cgccttcgag tgtgcaggaa
    12541 ctctcgaaca tgctgtacac gcttctgctg aagggtatgg aacatcccac cgccataaga
    12601 tatcccagag aggccgaggt aatctctctg gatgaagttc tctcatccat gaaagagata
    12661 gctctctgga aatgggaaga gatcatgcac ggcgaggaag gggcgatact ggctaccggc
    12721 tcgatggtgt cgcgtagtta tgaagtcgct ttgaaatacg gttacgcgct ttacaactgt
    12781 cgctcgatca aaccgctgga cgaaagtatg ctcgaatatg tgttcggcaa gtacgatacg
    12841 atcgtctgcg ttgaagaggg aatcaagaga ggtggttttg gctccggtat agcacttaag
    12901 gccatcgaac acggctacgc cggtaaaatc gatcttgtgg ccatcgacga ttgcttctcg
    12961 ccccacggaa cacgggacga gattttatct gaactggggc tcgacaccag gggaatagag
    13021 aaaactatct ctagactgag aggtgaaaaa aatgcaagtg actacggaat tcggtaaaat
    13081 cgatatttct cttcaggcca tctgttctat agtcaagaaa gtagtttccg aatcttacgg
    13141 tcccgtgaat gttggaacac cccagacggg ctttctggcc aaattattcg gaaccgccga
    13201 agataccaga aagggaataa aagtaaccga agagatcgac ggcactctgg aaatcgatgt
    13261 ggatctgatc ctcgaatacg gcgtgaggat tcctacggtt gtggaaaaca tacaggagaa
    13321 cgtcttccac aaactgaagg aactcacgga agccacgaat atcaaagtga atatacatgt
    13381 tgttggctta caggattgac gggaggcttt agcgatgaaa cgtatcaacg gcaagttctt
    13441 tgtcgccgcc ttccggaaag ccgctgaacg tcttctggcc aacaaggatg aaataaacgc
    13501 tttgaacgtc tttcccgttc ctgacggcga tacgggttcg aacatgtccg cggccatgat
    13561 agaggcatgt gagtatctgg acaggttgaa gaaggacgat ctggtcagcg tcctcgagtc
    13621 cgtcaagacc ggaatgttga tgggtgccag gggtaactcc ggtgtcattc tatctcaaat
    13681 attcagaggt tttgcagaag ggataggaaa cagaaaattc gtcagtacga aggccttcac
    13741 cgaaggtctt ataaaggcga aggaaattgc ctacaggtcc gtcatgaaac ctgtagaagg
    13801 gaccatgttg accgtcatga aagtcgtggc cgaaaccgcc aatgagcatt atacggagct
    13861 ggaggatttc gaagagtact tcgacgaaat ggtgaagacg gccttcgaca cggttgaaaa
    13921 gacacccagt ctcctgtcta aactcaaaga ggccggcgtg gtcgattccg gtgccaaggg
    13981 tcttgcctac atcttcgagg gttttcagct ggcgataaag ggagagctcg aagttgataa
    14041 cctgatacaa cagatgcctc aggcgatagg ctcttcgaca gagcgaatag tggaaatagt
    14101 aagagaggaa ttgaaataca cctattgcac cgaactgatc gtcaaccttc acgacagcga
    14161 gaatcccgaa gaaacctacg atgggttgaa ggccttcctt gaagagatgg gagattcgat
    14221 cgtcatggtc aatcaggacg agttcataaa ggtccacgtg cacaccgatc atccgggaga
    14281 cattatcgaa aagtttttac acgtgggttt tctccaaaag gtgaagatag acaacatgaa
    14341 ggttcaacac gaacacgttg tggatattca aacgaaggca tcggaaccct tcggaaaggg
    14401 taagaaacac ggtatagtcg tcatctcccc gggcgacggc ctggccgatg ttctcaagag
    14461 cctgggcgta gattacaccg tcaggggcgg ccagaccatg aatccaagcc tcaaggatct
    14521 gtacgaggcg ataagccgta tggaaccgga caaggtcgtt gtcctgccca acaaccccaa
    14581 catcatactc accgccaaag aggcggccaa cgccgttcat gacgagaatc ccaataagga
    14641 agtgtatatt attccgaccc gaacggtgca ggaaggtatc tcggccatga cggtgtacaa
    14701 cgacgattcc gatacgcagg aactgataga ggagatgaca caggcggccg acgcggttac
    14761 acccatctct ataacctacg ccgttcgcga ctcgagtatg aaaggaaaga aaataaagaa
    14821 aggcgagtac atcgccattg gaaaggacgg gcttctcggc tctggcaaaa agctggacaa
    14881 actcgtacag gactcgataa aagcggttat tggaaaggaa agcaacaggg aagtcgttac
    14941 ggtgttctac gggtccgacg tttccgaaga aacggcggca aaactggccg agacactctc
    15001 ttcgaatttt tccgatctcg aattcgagat ccacagcggg ggacaaccgt attactatta
    15061 cctgatttcc attgaatagt tcaggaggag aagatgtacc tacagaacgt aatagctaac
    15121 ctcaacaact actggtcatc catgggatgc atcatagacc agccttacga cgttgagatg
    15181 ggtgcaggga cctttcatcc ctccaccttt ttgagatctc tgggcaagaa gccgtggaaa
    15241 gtggccttca ttcagccaag cagacgaccg accgatggcc gttacggtga aaaccccatg
    15301 agagttcagc gctacttcca gtaccaggtg ataatcaagc cgaatcccga gaattcgcaa
    15361 gagctgtacc tcggatcgct ggaagcgctt gggataaacc ctgtcgagca cgatatacgt
    15421 ttcgtggaag acaactggga gtcccccaca ctgggtgcct ggggagtcgg ctgggaggtc
    15481 tggctcgatg gaatggaagt tacccagttc acctacttcc agcaggtcgg gggtatcgac
    15541 gtagatctgg tctccctgga aataacttac gggctcgaaa ggatcaccat gtatctgcaa
    15601 aagaaagaga acatattcga tatagattgg aacggagagt tcaaatacgg cgatgtcttt
    15661 ctggagaacg agaaagagtt ttccgcctac aacttcgatg tggccgatac cgcaaaactc
    15721 ttcgagttct acaggatgta ccgcgacgag ttcgacctct gtatgaagaa tgggcttatc
    15781 aggccctctt atgactatat gatcaaatgt tcccacgctt tcaaccttct agatgcgaga
    15841 aacgccataa gcgtctctca gaggcagagt tacatcaagt ctataaggga aatggcccga
    15901 tcggtggcac aggcttacgt tgacagggag ggtgaaagcg atgcataaga agaaagcgtt
    15961 gctggaaatc ggagtcgaag aacttccctc aagcgaagtc ctggcgataa ggaaccagct
    16021 cagagagaaa atcgagaaga gtctccaggc cagcagattg ggttacgacg gactaagaat
    16081 cttcacggcc agcagaaggt ttggactata catcgatggt ctctctccga agcaacccga
    16141 ttacctagaa gagaagaagg ggccgtcgga gaagattgct tacaaagacg gagagccttc
    16201 cagggcctta ctgggctttc tcaagagcaa cgacgcagga agtgaagata taaccgtcaa
    16261 agacggatac gtttacatag aaaggaagat accgggtaag tcgtcgctgg agattctgcc
    16321 cggtatattc aggcaggccg tgctgtcgct ggagttcaaa aagccgatgc gctggggcga
    16381 tggcacttac agattcgtca ggcccgtgaa atgggtcgta tccatgatag acaacaccgt
    16441 gctcgagatg gagctcttcg gaaagaaggc ctccgatacg actaagggcc acagattcta
    16501 cttcgacgaa atacgcgtct cacctgagaa ttactttgaa aaacttagag aggcgcttgt
    16561 gatcgctgac ggttccgaac gggagaggcg tgttctttcg gagatagaga aaaccggaga
    16621 actcctccag ggaaacattc ctgtcgacgg ggagttgctc gaagaagtgg tctccctcac
    16681 agagtatccc acggccgttg tcggacgttt catggagaag tacctggcgc tcccgccaga
    16741 ggtgataatc gtcacgataa aacatcacca gaggaccttt cccgtttaca gagaaggagt
    16801 gttgaccgat aacttcgttg ccttccagga cggcccggga gactctctgg ggaatatccg
    16861 gctcggttat gaagatgtta taaatgcaag gctcgaagac gccttcttct attacgagaa
    16921 ggatctcgag aaacctctgg aaagttacgt gccgggtctg gaagaaatac tcttccagaa
    16981 aggcctcggt tcgttcatgg acaaatctaa gagaacggcg gctctggcaa tcaaaattgg
    17041 aaagctgctc aaggcaaaga gtggggaact ggaaagtatc gaaagaactg ctcttctggg
    17101 caaggccgat caggtcacac gggtggttca ggaatttccg gaagtccagg gggtcatggg
    17161 aaggatatac gccgaaaaat ccggcgagag tgaagaggta gctctcggga tcgaagaaca
    17221 ttaccaggac caaaaagtac cctcgactct cagcggcgcc gtcgcgggcg tcgccgacag
    17281 aatagatacg gtcgtcggaa acttcacgat cggcaacatt ccatcggcct cgaaggatcc
    17341 ctacgcgttg agacgaaaga tggccttcat tttccggacg atgaattacc tgggatggaa
    17401 gatcgatctt aaagagctcc tgaaggagag caccggcatc ctcggcggtc aggcgttcga
    17461 tactttgcag tctgtcgcct cattctgtcg ttcccggtat gaagcctacc ttctcgagca
    17521 gggcttctcg atcaacgtga ccagggccgt cagcgactgg tggaggttcc cttatcttgg
    17581 cgagagagcc gcgaaggcta taaccgaata cgttgaaaaa gccgaattca cggatcttct
    17641 gatcgcttac cagagggttc acaatataag caagaaccac gattgcgacg cgttcgaagg
    17701 ctcgaagttc gttgagcagg ccgaaaggga cctcttaaac gagtatctcg aatgcttcga
    17761 gagcgtcagt tcggcccttg agaaagacga cttcgaaaag gcgctccagc ttcttacggg
    17821 attgaagtcg tcgatcgaca ggtacttcga cgatgtcttc gtgatggata accaggaaga
    17881 tataaggttg aacaggctgg gcttcctcaa atcgctcgat gggctctttc tgaaagtggg
    17941 ggatctctcc ctgcttctgg aagaagagag aaaatgagcg aaatcttcaa atccgggata
    18001 gttgccctcg ttggtaaacc caacgtgggc aagtcatctc ttatcaaccg cataatcggc
    18061 gaaaagatag ccatagtttc cgacaagccg cagacgacca gaaaccgtat cggcggtatc
    18121 tacacgaccg atcaaggtca gatagtcttc tacgacactc ccggaataca caagcctcta
    18181 cacaaactcg gccagtatat attgaaagtc gcgacctcct ctctgctggg cgtcgatctt
    18241 ctactggtca tggtcgatcc caccgacggg ctcagggaat cagacagact ggtcgccact
    18301 cacgtcaacg gaagccacgt cccggtcttt ctcgtgatca acaaaataga cgcgtacggt
    18361 gacagaaaac tcctggagaa cttctcggaa aacgcctccc gtcttttcga gaatgttctg
    18421 gaacgttttt acatctctgc cgaaaagggc accggcgtcg attcactgat agaagcgatc
    18481 ttcaaattcc ttcccgctgg aaagatgctt ttccccgaag attacattac cgatcgttcc
    18541 tcaaggttca tggcctcgga aatcgtcagg gaaaaagtcc tccagcatac ccgccaggaa
    18601 atcccgcatt cggtcggcgt ctctgttcac gagttcaaag atgagggcga tttgctgagg
    18661 atcagggccg atatcgtggt ggagaggaat agccagaagc cgataatact cggcaaaggc
    18721 ggttctatga taaaactcat cggtagcgac gcccgaaggg acatggagta catcttcgat
    18781 cagaaggtct ttctcgacct ctttgtgaaa gtcagagaaa agtggagaga taaggattca
    18841 atgatacagg agttcaccaa tctgaaagac gaactcagct gaaaccgggg gggttagtat
    18901 gaaaaagagt cgggccagga tcgtactttt tctcatgctc ttcatgatgg tttttctcaa
    18961 cgcggatcag atggtaatgt ctccgaacat cggagagata gaggccgagt tcggtataac
    19021 caaggccgac atcggcctga ttcagggatc attcacgatc gtgggagccc tggtctcgct
    19081 ggtctggggc tttctggccg ataagtacaa caggaagctt ttattgttgc tgagcgtgct
    19141 ggtcggagag ataccctgtt ttctttcggc tttcgttcag agctttccac agttgttcat
    19201 aaccagagct ttgacgggaa tcggtgtggg ggcactcttt ccggtcgttt tctccttcgc
    19261 gggagacacc ttcaaagagt cgcacaggcc aaaggtcaac gcctttctct ccacagccat
    19321 ttctctgggg gcgatcgtgg gaatggttat cgccgggttc accggctcga cgctggggtg
    19381 gcgtattcca ttcataatcg tctctcttcc aaacatcttt ctggccctgg ccttctactt
    19441 cctggccgag gaacccagga ggggcgccgc cgaggttgcc gttggagagc ttgtagataa
    19501 aggctacagc tacgccggga aggtgaagct ctccgattac ctcaacctct tcagagtcag
    19561 gacgaatctg gtactcttca tacagggaat actgggcacc attccctggg gtgctatccc
    19621 ttactacctg gtcaactact tcgagactta caaaaacctc tcaaaagaat tggccacgct
    19681 tatcttcatg tttttcggcg tggggaacgt tctcgggatt tttctgggcg gcctgatcgg
    19741 cggtctgctg tacaaaagga aaccttccta catgccgctt ttcagcggcg gtatgacgat
    19801 cgccggaacg tttgttgccc tgggtgcctt aaactttccg ccggttgaag gtacgggcgg
    19861 ttttctcatg ctgggacttc tcgggatggt ggcagccgcg acggcctcga tgaccggtcc
    19921 aaacatgaaa accatgctta tgaacgttaa cgagccggaa agcaggggca ggatcttctc
    19981 tgtcttcaat ctcaccgatt ccctgggcac agggttcggt caattcttcg ccggcactct
    20041 cgccacggcc gtcggttctc tgggcgtagc catgaacgct tcggcccttt tctggttgcc
    20101 ctgtggtctt gttcttctcg tcgcagcttt gaccttcccg cgggatatca ccaggttgca
    20161 cacgaggatg caggaagtgg cgaaaaccat ggaaagtgtg aaataaaagc gatctggaaa
    20221 atttttatac cgaatggatt ttcgattttg attctgggga aaaaagttgt ataataaaga
    20281 tgctgaagta accctcagac cgcttcgctg ccctcgaaag gggcagcaat tttttttaca
    20341 tttcggccta tctcctcccc tggcgcattc aaggcctctc cgatttcact cgaatcgagt
    20401 ttccggcaag atagccggtc gagaaagccg cctggaggtt gtaaccacca gtctctccgt
    20461 cgaagtcgag aacctcgccg gcgaagaaga ggctgggaac cagacgcgat tccatgctcc
    20521 tggagttaac ctccaggatt gaaatccctc ccctcgtcat catggcggtt agaaagtccc
    20581 cggtcctgga cactttcatt tcgagaccgg ttaggagtct ctccagttcc ttcctctcct
    20641 gcttctttat ctccgctccc gttctcgccg ctgctccaga gagttccacc agcttttcgg
    20701 tcaacctctc cggaaggtcg agatcggcga ggatattctt gacgttcttc ttgccgttgg
    20761 cggtcaattt ctccagtagc agcctgtcgg catcttccct cttccagccg gcgagattga
    20821 taagcagagt gtcatccggg agtatctctc ttgagagatg cagaatggat ggaccggaca
    20881 gtcccgtgtg ggtcagcagg agatcctctc tcctttcggc tatcttgccg ccgtttctcc
    20941 agagtgagac ctctgcccgc tttatggaga tgcctgagag atccgagaag ggaaaatcgt
    21001 ctatatatac ggccgccagc gcaggcctcg gctcctgcgt cgtgtgcccc atcttttgcg
    21061 caaagaggta accgtcacct gatgagccgg tgtaggggta gctcttgccg ccggtcgtta
    21121 tggccagata gttggcctga agatctccac ccggggtctt gaccagaaag gtcccgtcgt
    21181 cgtctctatc gacggaagca acccggcaat tgcactggat gactataccc tgcttttcac
    21241 actctgccag gagcacttcc agcacatccc tggcgctgag ggttacggga aagacctttc
    21301 catcgtctct aacggtcatc ttcagccctc tcttttgaaa gaattcgatc agtcttgaac
    21361 tgtcgaaagc catcagggca ggcttcagaa acctttgcgc ttcgatcccg aagtaatgct
    21421 tcagcatcgc cttcggagcc tcagaattgg tgagattgca ctgtcccgat cccgtcatca
    21481 gaagcttttt accgggcgag tcgttcttat cgagtacggt gacgtttcta ccctcggtcg
    21541 tgcagcttat ggcagtgaag aggccggccg ggccggcgcc tataaccagt atatttctca
    21601 atttaaagac ctcctatcgt gtctggccgc cgttttcgaa aggggcggca atttcaaagc
    21661 tcatgaacgc ttcatcgagc gaacccggga aagaccggca acaatctcgc tccgtgcgct
    21721 gaacagaaag aacgcgaaga aaataacgtt ccccagcaga agccagagca gggtggtgga
    21781 aaaatacttc aaggcgaaga caaaaagata gagagacacc gcgcaagtca agagcagcat
    21841 gatcctcctg aagggcatga aattcatccc ttcctttctt atcaggaggt tgtaaatcac
    21901 cgtcgaattc agtgaaacga gcgaagtgct ggctgctatg cctgcgtggc ctagaggttc
    21961 cagcagcaac cagtcgaaga gaacgttgag catgatggat ataaaagata taagggttat
    22021 gaagcgaacc ttcctccggg ctatgaagta gctggaacag gtgtacgata ccggtgaaat
    22081 aactatgagc gcgccgtacc ctatcagggc catgctgacg agcctcgtcg aatcggcgtc
    22141 gaactctcca cgctggtata tgaatctcac cagaggttcg gccatcgata tcacccatac
    22201 cgccagaggc agggcaatgt taagcgagct cgtgacggtt tttcgcaacc tctcacgtgt
    22261 cttttcacgc tctccggata cgatgaattc cgagagctcg gtgtaggaag tcatcaaaaa
    22321 ggccgtaaca acgaacgtga tcagtcctaa aagcgtgtgc gcgtactgga gagacgagac
    22381 cctcccgggc ggaagcagag agacgaaggc tttatcgacc attccattta tcaggctcag
    22441 actgccagag aggaccaaag gcagagaaag ggtgagcgtc tcgactattc tggtgcccct
    22501 gtcaaacttc aacacgaaga gatccctaga ctggagagcg tagagcaaaa ccgtcagggc
    22561 gtttccgacg atccacgcca gtatatacga ggcttccgag agaaagggag aggtgaagag
    22621 gataaccggt atggccacaa cgttgaagat caattgggag ataccgaact gaatgaatct
    22681 cctgcttgcc ttcaaaagag ttcccagaag gttttccagc ccgcctatga gcggatatat
    22741 cgaaaggaat ctcagcttcc tggcggcgta agcgatggtt tcctcagaaa agccaggagc
    22801 gaagagtctt atcaccagat cgggtatcag caaaaaaact atgcccatgg agagaaagac
    22861 agcgcttccc gccaaaaaca cggcaccggc gtattttttc gatcgaaggg gatcttcgtt
    22921 ccttatagag tgatagatgg gtacgaagat cgaagccagg gctccggcga ctatgctgga
    22981 gagagtggtg gctggagtca tggcgatgaa cacggcgtcg agattgccag aagttccgaa
    23041 aagaccggcg atgagcatct ctctgaaaaa acccatcccc ttcgatatga ttgagaatgt
    23101 gagtatggcc agcgttccaa cggcaactga gctggatcta gatattcttc gcatgctttc
    23161 ctccgatctc cctaacaagt atatgatagt tatcgctttc tttcctgcag gaatgctaga
    23221 atttcagcgg gaggttcacg gccgatgaga aggttaccgt tc
//
LOCUS       DAOOLY010000005        22436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_2233_length_22436_cov_4.617443, whole genome shotgun sequence.
ACCESSION   DAOOLY010000005 DAOOLY010000000
VERSION     DAOOLY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 22436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22436
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_22436_cov_4.617443"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLD15_00520"
     CDS             <1..393
                     /locus_tag="PLD15_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB62407.1"
                     /translation="ITGIHGLRNEDVSEEPPMAEVFPKFKDYIGTSVLVGHNIVNDMT
                     FFDIASKETGLMPLSNNYIDTIELAHEAFEDGPFNLYGLARRLRIRDVPTHRAMDDAR
                     VTAKVFLALARYLGGLSEVGKFVKKWRG"
     gene            395..1681
                     /locus_tag="PLD15_00525"
     CDS             395..1681
                     /locus_tag="PLD15_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPB62408.1"
                     /translation="MVKDFVLDTNVLVHDPYCFMNFEDNNIIIPFPVLEEIDKLKKNA
                     GSVGQNARKVSRYLDSLREKGSLAEGIKTESGGTIRIAVFNEFKSKLPPFASNGYKDN
                     AILLYTMELVDSEKFSVVLVSKDINMRVKADILGLKAEDYLHDRVEIDDQLSGVETVR
                     DPRLMERFLLEEGLPAGEVKEGLEANEFVDFGDGVVGRVSPDRENVVPLRLDMESSSW
                     GIKPRNLEQLMAMELLLDDDIKIVFIPGMAGTGKTLISLACGLRKVIDEKRYERLLVA
                     RPIIPMGQDIGYLPGSMEEKMSPWMTPIIDNLHILFGNRRMDLETFMKKGEQLQVEVL
                     SYIRGRSIPNQYFIIDEAQNLSPHEIKTIITRVGENTKIVLIGDPYQIDNSYLDAFSN
                     GLTYAASRMVDRPIAGHITLTRGERSELASLAAELL"
     gene            1694..1939
                     /locus_tag="PLD15_00530"
     CDS             1694..1939
                     /locus_tag="PLD15_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HPB62409.1"
                     /translation="MNEGPREYDLFVRIRPEDIHVLCYIAEAEDNLLNIRHVTDDGFL
                     KIIVPGDLLEEVMNFLESIKKKIDLEVVEIRENPGHT"
     gene            1899..2969
                     /locus_tag="PLD15_00535"
     CDS             1899..2969
                     /locus_tag="PLD15_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HPB62410.1"
                     /translation="MKWWKYVRILDILDNAVPKEKAENVREIDYDRLQRLGYNTILFD
                     YDNTIAVWREPFDMRNKEVIDRLIQSGMKVGVVTNGPQSRVRNLKEIFGNQLKVYHSM
                     RKPGTKELRRVLSEMKSKPEKTVIIGDLFFTDIIAGNRMGMYSILVSPIVDISQKWYK
                     RVMARISIGVYLLFFFTVGWIFRINHLATPHRFANNVMDIDFDSLKESGYRLILFDFD
                     NTLEDWGASTVSREKKLLLSRIERLGMSVVLVSNGKKGRLGKIDDELATIKVLSRARK
                     PLTFKAKKVLKDLEIPPYQTIVVGDQLFTDIIMGNLLGAYTIKTEPLSEREFFWTRLV
                     RRVERMLLKNMKKHPSVEAMDR"
     gene            2966..4066
                     /locus_tag="PLD15_00540"
     CDS             2966..4066
                     /locus_tag="PLD15_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPB62411.1"
                     /translation="MKCKGCGVELQTVDETGPGYISEKVLNERLSDGRPVLCKRCFSM
                     KHYGRLDAENNLVHSLENLKAYLKTTGDILYVMDLSDFNGTFRGDILSLLTDHRVHYV
                     LNKVDLLPLEVKIEEIKIWAIDMLKVPERRVRLASSLNGYGINSLYAYLKENGENYTA
                     VGVTNVGKSSLLNALTRSSEITVSRFPGTTLEVTPQRLERAGITVYDTPGVFTSDRVS
                     DLLSVSDQSVLLPRKKLERYTMQFHSERTVFAGGFVRIDAVNNSDPVGIFHLFMPESV
                     SIHETNSSIADAQWDSWFGSFLKPPFTGSARSALTWKRERYSLKTGQELHVNGLGWIN
                     IAKGPVSLSVTLPESVRLTVRKGLVGPKKFKR"
     gene            4087..4680
                     /locus_tag="PLD15_00545"
     CDS             4087..4680
                     /locus_tag="PLD15_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPB62412.1"
                     /translation="MKLKEPAIKAGVSNRHIHLCAEDIEILFGKGHQLTPIKDLGQPG
                     QFACDEKVIIVGPKGSLTGIRVLGPARKATQIEVSRTDAFALGIKPPIKDSGDHENTV
                     GLTVVGPKGTIVLKSGVMLAKRHIHMTPEDSLVYGVKDKDIVMVYCKGDGTRKTIFDD
                     VLIRVHPTYALEFHVDVDEANAAILNNNDPVYIIEEL"
     gene            4680..5474
                     /locus_tag="PLD15_00550"
     CDS             4680..5474
                     /locus_tag="PLD15_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPB62413.1"
                     /translation="MIHIGTSGYSFPDWIGTAYPKGLVSSQMLRYYHNVWKFNAVELN
                     FTYYRMPSYKTITGMLREIAGEMVFAVKAPGKITHELWKAPSADLMELAVGFLGALSP
                     MREEGRLGPILFQFPWSFKKSEDNMAYLERLSKIFDSGENRLVVEFRHDSWAGEESFE
                     FLGKIDAVPAVVDEPLIAGLFPYLPRQRRNVAYFRFHGRNPGWFESEGSERYNYDYSE
                     NDLRSFALDVLEFHGHGLEVFVFFNNCHLGRAVHNALRFRDMIGGA"
     gene            5482..5811
                     /locus_tag="PLD15_00555"
     CDS             5482..5811
                     /locus_tag="PLD15_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62414.1"
                     /translation="MTFPDYVNMVLKMIAEGNENHEIFEQLQKLGLREGEITEVYNAV
                     VGMISSGPESTELIIDELYDPRRRVFLEEELEAIKAVPGLYKALVWGEITPGDFERTL
                     SDFSSGN"
     gene            complement(5838..8405)
                     /gene="glgP"
                     /locus_tag="PLD15_00560"
     CDS             complement(5838..8405)
                     /gene="glgP"
                     /locus_tag="PLD15_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPB62415.1"
                     /translation="MYFLERVMAVPRLPEKLARLEELARNLWWSWNYDAQEMFKYMDP
                     EMWYQEKRSPVRLLRRIKQERLYELEEDRQFLAMYENTLKNFDQYMGNENTWLERNHP
                     DRRERCVAYFCAEYGFHESFPIYSGGLGILAGDHLKTASDMGLNFVAVGLLYKNGYFE
                     QRIDESGWQQNIYSRYDFEDFPVVPAVDENGDEVYINIDLPGRKLWAKIWRVQVGKTA
                     LFLLDTDIPQNEAEDRKITSNLYGGDREMRLKQEILLGIGGVRALRVLGINPELWHMN
                     EGHAAFLSLERIREYVQGKGLDFATAVTLVKSGNVFTTHTPVPAGNDAFDLETIERYF
                     RGYWEKMKTNREEFMELGLEKFPNGGQHYSMTVLALKLSTASNGVSELHGKVSRNLWN
                     HIYPDLPAVEVPITHVTNGVHIWTWLNSTLVKLLDRYLPDDWHERVDDPEVWERVDDI
                     PPEEIWQLHLALKSRTKSFLQTRLKRQRRRLGETIEDLMEVDEILPEDVLTIGFARRF
                     ATYKRATLIFKDVNRLKSIISSSEPPVQSVSAGNAYPADDPGKELIRKLYEISRTPEF
                     KNRIIIVENYDMNIARHLVSGVDIWLNTPRRPHEASGTSGEKAGMNGVLNFSVMDGWW
                     VEGYDGNNGWAIGDNRDYQDHELQDRIDSVSIYSALEKEIVPLYYSRDEKGTAVEWAQ
                     RMKDSIKEIGKRFNTQRMLADYAEKLYFPVIDLFDEVQKNDFRLAKNVSGWKEKFSAS
                     WNAIRIKPNIQVESTTRSIKVGQEVSLSANIYLGTMDPKEVLVEIFVARMDDNGEMIN
                     FKLYPMSLIKEDVHGEYIYGGKFTIPEEGKLAYTIRVVPNPDHLPDRYFIPLARWIS"
     gene            complement(8582..8950)
                     /locus_tag="PLD15_00565"
     CDS             complement(8582..8950)
                     /locus_tag="PLD15_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB62416.1"
                     /translation="MSVKVGTVVEGSVASVKKFGAYVTLEGGEEGFIHISKVAREYVK
                     NIEDYLKVGQKVEAKVLGTTKDGKWELSIKDLKSKGSDEGPSNQDFEKKLAKFMRDSG
                     EKISAFKRRLDKKRGIRKRP"
     gene            complement(9004..9213)
                     /gene="rpmE"
                     /locus_tag="PLD15_00570"
     CDS             complement(9004..9213)
                     /gene="rpmE"
                     /locus_tag="PLD15_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPB62417.1"
                     /translation="MKKGMHPEMKLISVKCTCGAEHKFYSTKDNIKIDLCSSCHPFYR
                     GDASSMIIDTEGRVQKFRNKYGDNY"
     gene            9325..10326
                     /locus_tag="PLD15_00575"
     CDS             9325..10326
                     /locus_tag="PLD15_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HPB62418.1"
                     /translation="MNAAIRSRIIDNVSSEGFYSFYGKRKDSLERYAKFLKKNPLERT
                     VLGKLKRIIPELSGLSFEELEFTIDILRERDRSPLERVEYVSGLMNLPARPVGHLLFI
                     VDPRNNPPVNGLLKGEVESLEDYARWIEETGSLQEMGVMNYIMLESALCFKKETSEEL
                     DIDVRIKATDFTNLKELRTLREEVQSLDRELLKRLTRELKSVHPYVRSVLFSRSHRQV
                     VIDGSNIVYSRQDAPDLARLDDLFVNMAKSRVALFPFRVVFDGNIAYTIGGFQQERLA
                     RWLSLPQVETYSPADEKIIRLARQHDAVVITYDRYLEHGAGDLILVRPEEIDENLGL"
     gene            10307..11317
                     /gene="queA"
                     /locus_tag="PLD15_00580"
     CDS             10307..11317
                     /gene="queA"
                     /locus_tag="PLD15_00580"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPB62419.1"
                     /translation="MKTSDFDYYLPEELIAQKPAVPRDSSRLLVIDRARESFDHRSFR
                     EIIEYLNPGDTLVMNDSRVIPARLIGRKDTGASAEAVLIEKIDRGLWKAIVRPGNKVK
                     KGSRLIFGSLDCEVVEHCDDSSRLLDFKGARDDEIKLVGRLAIPPYIHTYPEDPESYQ
                     TVYSKREGSIASPTAGLHFTSELLEIISEKGVGIEYVTLHVGIGTFRPVKSERIEEHQ
                     MHEEYYTLSSETVEAVNRTRERNGRVVAVGTTTVRTLESVVSRHGRLVEDSGSTDIFI
                     YPPYKFGVIDSLITNFHLPKSTLLMLVSAFAGRELIMEAYREAVEKRYRFFSFGDACL
                     IL"
     gene            complement(11605..13038)
                     /locus_tag="PLD15_00585"
     CDS             complement(11605..13038)
                     /locus_tag="PLD15_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB62420.1"
                     /translation="MLRNIIKQPRFIAAVFTLIYLIIGLVVFSISLRTAVIVFSTFTV
                     VLFLEPWTRLAQKYFKIKRSLSLGFGLVIIFVGLITLIVFLTTPLAKEASKFYNIVVD
                     FFPSVEETKITSFEVNTNIDRLLSDENFATGLTQEEKGSLEFLSKDLKSYYAEIMKTV
                     PTMETPFEKELKSIEESLREKGNYIVIMSSDQTREASYEELNDVTSRYLSPSNAAMLS
                     QELLANAMQVQSQELWREVVAYFMPKNLDPARQAATYESVRNFLIQVQNILREFLPSL
                     LEKVPSFITSTGLVIFFTIVGAIYLSYYFLSIKEIVPKLYPKKVRKIATEFLSDTYKN
                     LERYVISIVLVAIIVGIVVGIAIKAMGLKYSLLMGLWAAFTNLIPIVGVPLEFIPLLL
                     LAVSTQNIVLVLILLIVLTIVHAAAFILFLVFMKGYNRINPVMIILMIVVAGQLFGLF
                     GAIIAVPFAILIKMLWIHFVSPLLDEE"
     gene            complement(13040..14035)
                     /locus_tag="PLD15_00590"
     CDS             complement(13040..14035)
                     /locus_tag="PLD15_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPB62421.1"
                     /translation="MKGKVVIITTGGTIAMITDPVSGASIPAQGINKHVANIKGLDEL
                     ADVEHIEFSNVPSPYINPDTMWKLSRLVFKTLSRDDVAGVVITHGTDTLEETAYFLDL
                     TVNSEKPVVCTAAMRNIDEMGTDGPRNVLASVRVVLNSLAIGMGTTVCLNEEIHAARE
                     VTKTYTSNVATFDSPGHGPLGIVDEDTVIFFRKPLLRATFNVERIENRVALVKTFTGD
                     DGSILKSLPELGYKGVVLESFGRGNVPVEVYHVVKYLIEERGMPVIITSRCFKGRVLG
                     VYGYIGGGKSLADIGAIFAQELSSMKARIKLMIVMGITDNREEIERLFRLPLKGA"
     gene            complement(14032..14367)
                     /locus_tag="PLD15_00595"
     CDS             complement(14032..14367)
                     /locus_tag="PLD15_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62422.1"
                     /translation="MIPAFNEGQIFFGRPLGTYPILVGSPVSFKGKTDIAVTDHGMRS
                     ITGLPLGTDIDSLGERELRYIPGIGRDRARTIVFKRPSVTGELAEIVGSDTIKTMSKL
                     GLKLGGKDI"
     gene            complement(14364..14618)
                     /locus_tag="PLD15_00600"
     CDS             complement(14364..14618)
                     /locus_tag="PLD15_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62423.1"
                     /translation="MHIDNGNPIFIARHERETRKALESIVRYDTCGDTISFGVESFDT
                     RVRELNNLGGSVEDIRRAIEPVNEIGGQRVDGIPKLLPEE"
     gene            complement(14623..15012)
                     /locus_tag="PLD15_00605"
     CDS             complement(14623..15012)
                     /locus_tag="PLD15_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62424.1"
                     /translation="MKALIVDGYIDEPAAFGVPPYISPRIKVVAGAFLLKGFDVDYLT
                     IDLVRERDQWNRCLDYDYLVIHGGITTPGRYLGGTPISSTEISRLLNLCDGPVRIVAG
                     PIVSAGYTLRGGTTAFNPDFSQRGTRS"
     gene            15083..16297
                     /gene="clpX"
                     /locus_tag="PLD15_00610"
     CDS             15083..16297
                     /gene="clpX"
                     /locus_tag="PLD15_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPB62425.1"
                     /translation="MLPFERHCSFCGRPSSQVKKLISGTEGYICNECVDLFHDIIQQD
                     QKAKKGPGKKLPTPADIKMELDKYVIGQEKAKKIISVSVYNHYKRVFNDVEDVEIEKS
                     NIMMVGPTGSGKTLIARILARILDVPFAIADATPLTEAGYVGEDVENIVLRLLQSSNF
                     DVEKAQLGIIYIDEIDKIARKSPNPSITRDVSGEGVQQALLKIVEGTVANVPPQGGRK
                     HPYQEFIKVDTSNILFIVGGAFDGLDSIIKQRVLDSSMGFGAEVRSKNQLRLGQILQE
                     AVPDDLIQYGLIPEFVGRFPVLATLNDLDAEDLKRIMSEPRNAILKQYQKLLGLDGVE
                     LEFTDAAMTAIASKAMKRGTGARALKSVIEQVMLDIMYDIPAMTNVEKVVINEDVIIK
                     NASPKIISRESA"
     gene            16297..17289
                     /gene="tsaD"
                     /locus_tag="PLD15_00615"
     CDS             16297..17289
                     /gene="tsaD"
                     /locus_tag="PLD15_00615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB62426.1"
                     /translation="MKILAIESSCDETAVAIVEDGRVLSSLVSSQIDVHKKYGGVVPE
                     IASRKHLEIVDRLVLQAVEETGLTLEEIDVFAATMGPGLVGSLLIGLSVAKGLAISLN
                     RPFIGVNHLYGHVYANLISCPDLRPPFMVLLVSGGHTEILLMEEWNTVEMVGRTRDDA
                     AGEAFDKIARLLGLGYPGGPEIEKAARGGSPVYSFPRPLQEKGNYDFSFSGLKTSVLY
                     FLQRNPGVSVEDVAASAQEAIVDSLVTKTFDAAQSLAVNKIALAGGVASNSLLRERAI
                     DMASKCGIRVFFPPIGMCTDNAAMIAVAAYEKAKRGLYSPLSTNAVPYLSLDVF"
     gene            complement(17290..17514)
                     /locus_tag="PLD15_00620"
     CDS             complement(17290..17514)
                     /locus_tag="PLD15_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB62427.1"
                     /translation="MPFYRYECEDCGKTTEAFQKMSDEPLKVCPSCGGHLKKLLSSVG
                     IVFKGGGYYSTDSKKTSSSSSTSTVPSKKD"
     gene            complement(17563..17925)
                     /locus_tag="PLD15_00625"
     CDS             complement(17563..17925)
                     /locus_tag="PLD15_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB62428.1"
                     /translation="MPDSIQVGKAHEVKPQLIDNEKVKKVQKRVLIGKSKGAKNFVMR
                     LFTVEEGGYSPLHSHPWEHEVYIVSGNATVVTQEGEQPAQACSYVFVPANAVHQFKNS
                     GKGNLEFICVIPNTADEE"
     gene            complement(17891..18604)
                     /gene="jag"
                     /locus_tag="PLD15_00630"
     CDS             complement(17891..18604)
                     /gene="jag"
                     /locus_tag="PLD15_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPB62429.1"
                     /translation="MKSYQFKADSVEEAIRLARRELDAYEDELDIKIIEKGSKGFLGI
                     FGTKESVIEVSLHDSHSERKLYEYLKGILGNFDEEVLSEVKLVGRTYRVKIEGEKISR
                     LIGKHGKTVAALQHILNIYANRMAQIKVNVTVDVGDYRYERKKLVEEIAHRTAKSALS
                     KKGKIVMEPMFSFERRLVHEVISQYHDLKSYSVGFEPYRKVVVEYSERKYRDDRRKSD
                     PVRRRGYGDAGQHPSRQSA"
     gene            complement(18634..19980)
                     /locus_tag="PLD15_00635"
     CDS             complement(18634..19980)
                     /locus_tag="PLD15_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPB62430.1"
                     /translation="MRKTLIMLVLLLSVFTLSLMGADLITQEGTNTLIVSGKTLRIVI
                     DIEKGLLTGVTSFADNKGVEFYAYNESLGKDGFNIFDQQGNELLPQSFSRTTDAEGNV
                     VVDFFYQQGRKSFVILNSPYYDFNVVVDFNQSVEVALPFISYNNQTLGPDSSYYLTYD
                     KFSGQKTLFAIASQQGSFKFQRYASDSGKGILEVFAGPMRLIHVKEALPEYYSALVKD
                     LEGFGAVNFFSWIYHGIVYFLFWLYQWSGNFGWALILFTIATRFVLYPLYHIQTKSML
                     MMRMLQPEIEKIKNKYKDPKRQQEAMMALYKEKGYNPASGCFPILIQLPILIILYNAI
                     RYFSEVMAYTPGFLIWSDLSVGDFIQNIPFIIITIVVGVFSSLLTAQDARSARSGVIM
                     QLIFPILFATFPSGLLLYWTTQSVLQLLISWVVYKRHGIKGLTLRQMLGLPEKPAK"
     gene            complement(19977..20219)
                     /gene="yidD"
                     /locus_tag="PLD15_00640"
     CDS             complement(19977..20219)
                     /gene="yidD"
                     /locus_tag="PLD15_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPB62431.1"
                     /translation="MKKIVLFLIDFYRKKISPRTRPRCRFYPTCSLYTYEAVERFGAF
                     KGLILGTWRILRCNPFNPGGIDEVPDHFTLIRRHER"
     gene            complement(20179..20592)
                     /gene="rnpA"
                     /locus_tag="PLD15_00645"
     CDS             complement(20179..20592)
                     /gene="rnpA"
                     /locus_tag="PLD15_00645"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPB62432.1"
                     /translation="MKSEDQSLKKTEIIRRKADFDRVFKKGKSIVDPFFVALFVQNSL
                     PFSRIGVSVKRKFGKATLRNRLRRLVKEVYRTGKEDFPEGYDILFIARKDLSDLFRRR
                     EVSFFEIQRVLRRIANKIGEMPDEKDCTFPDRFLP"
     gene            complement(20589..20723)
                     /gene="rpmH"
                     /locus_tag="PLD15_00650"
     CDS             complement(20589..20723)
                     /gene="rpmH"
                     /locus_tag="PLD15_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPB62433.1"
                     /translation="MKRTYQPSKVKRNRTHGFLVRSRTVGGRKVLASRRRKGRKRLAV
                     "
     gene            21025..21852
                     /gene="pheA"
                     /locus_tag="PLD15_00655"
     CDS             21025..21852
                     /gene="pheA"
                     /locus_tag="PLD15_00655"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPB62434.1"
                     /translation="MKDTVAFQGEHGAYSEQAIRKLLGESPVSLPCRSFQEMLYFVIE
                     RKADYAILPVENSLAGTVIPAYDALIDSDLLVQAEVMLRIEHCLMAPKGRSIEDIKYV
                     ISHHQALAQCSKHIEEEGFEAKEYYDTAGAARDLATSEMPFTAAIASELAAKTYGLHV
                     LRRNFEDMETNTTRFFLMGRGAVKCTEGCKTSIMFTTEHLPGALFRVLGELSNRNLNL
                     TKIESRPFKREMWHYLFFVDFEGTVATRKVEEAIESIRSLCRYFKFLGSYRSAGSET"
     gene            22098..>22436
                     /locus_tag="PLD15_00660"
     CDS             22098..>22436
                     /locus_tag="PLD15_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB62435.1"
                     /translation="MLLSLAGRNFFTFREFYVEFSSGMNAITGESGAGKTIFLKALWT
                     AVGFPPPWAGEESGNIEANFSIEDSILRRIGEFGTEIGDGNQLLVNVNFTGQRTIYRL
                     NGRMVPRQMIQ"
BASE COUNT         5616 a         5574 c         5641 g         5605 t
ORIGIN      
        1 ataacgggta tccacggttt gagaaacgaa gacgtctctg aagaaccccc catggccgag
       61 gtctttccta agttcaagga ttatatagga acatcggtgt tggtgggtca taatatagtc
      121 aacgatatga ctttttttga tattgcatct aaggaaaccg gtcttatgcc gctttcaaac
      181 aattacattg acaccataga actcgcccac gaggccttcg aagatggtcc tttcaatctc
      241 tacggactgg ccagaaggtt gaggatacgt gatgttccca ctcacagggc gatggacgac
      301 gcccgcgtga ccgccaaagt cttcctggcg ctggccagat atctgggagg gctttcggaa
      361 gtgggcaagt ttgtgaagaa gtggaggggt tgatatggtc aaggacttcg ttttagatac
      421 aaacgttcta gttcatgatc cttactgttt catgaacttc gaagacaaca atatcatcat
      481 tcccttcccc gttctcgagg agattgacaa gttgaagaag aacgcgggat cggtcggtca
      541 gaacgccagg aaggtgagca gatatctcga ttcgctgagg gagaagggca gtcttgccga
      601 ggggataaaa acggaatcgg gcggcacgat acgtattgcc gtcttcaacg agttcaaaag
      661 caaactgcct cccttcgcgt cgaacggcta caaagacaac gccattctgc tttacactat
      721 ggagctagtt gacagcgaga agttctccgt cgtactcgtg agcaaagata taaacatgcg
      781 cgtcaaggcc gacatattgg gcctcaaagc cgaggattac cttcacgaca gagtcgagat
      841 agacgaccag ctttccggtg tcgaaacggt gcgcgatccc agacttatgg aaaggttcct
      901 tctggaagaa ggtcttcccg ccggagaggt caaggaaggt ttggaggcca acgaattcgt
      961 agatttcgga gatggggttg tcgggagagt ctcacccgac agggagaacg tggtgccttt
     1021 gaggctggat atggagagct ccagctgggg tatcaaaccg agaaacctcg agcagctcat
     1081 ggctatggaa cttcttttgg acgacgatat aaagatcgtt ttcatacccg gtatggccgg
     1141 aacgggcaaa acgctgattt ccctggcctg cgggctcagg aaggttatag acgagaaacg
     1201 ttacgaaagg ctcctggtgg ccagaccgat aatccccatg ggtcaggata taggatacct
     1261 tcccggatca atggaagaga agatgagccc ctggatgacg ccgataatcg acaatctgca
     1321 catccttttc ggcaacaggc gcatggatct cgaaaccttc atgaagaagg gggaacaact
     1381 ccaggttgag gttttgagtt acataagggg ccgatctata ccgaatcagt atttcataat
     1441 cgatgaggcg cagaacctct cgcctcacga aataaagact ataattacaa gagtggggga
     1501 aaacacgaag atcgtcctga taggagatcc ctaccagata gacaactctt atctcgacgc
     1561 tttcagcaac ggtttgacgt acgcggcttc gaggatggtg gacagaccca tcgccggaca
     1621 cataaccctg accagaggcg aaaggtccga gctggccagt ctggccgccg agcttcttta
     1681 gtagaggtgt tcaatgaacg aaggacccag ggagtacgat ctcttcgtaa ggataaggcc
     1741 ggaggacatt cacgtcctct gttacatagc cgaggcggaa gacaacctcc tgaacataag
     1801 acatgtaacc gacgacggtt ttctaaagat aatcgttccg ggtgatttgc tggaggaggt
     1861 aatgaatttt ctggagagta taaagaagaa aatcgatctt gaagtggtgg aaatacgtga
     1921 gaatcctgga catacttgat aacgccgttc ccaaggagaa ggccgagaac gtgagagaga
     1981 tcgactacga tcggctccag cggcttggat acaacaccat tctcttcgac tacgacaaca
     2041 ccatcgcggt ctggcgcgaa cctttcgata tgagaaacaa ggaagtgata gaccgcctga
     2101 tacagtcggg catgaaagta ggagtggtga ccaacggacc gcaatcgagg gtcagaaatc
     2161 tcaaagagat ctttggcaat cagctaaagg tttaccattc gatgaggaag cccggtacca
     2221 aggagctgag aagggtcctg agcgagatga agtccaaacc ggaaaagacc gtaataatcg
     2281 gagatctttt cttcacggac ataatagccg gcaaccgtat gggaatgtac tcgatcctgg
     2341 tatcgcctat cgttgatatc tcccagaagt ggtacaaacg agtgatggcg agaatctcta
     2401 tcggtgtgta cctccttttc ttcttcaccg tagggtggat tttcaggata aaccacctgg
     2461 ctacgcccca caggttcgcc aacaacgtta tggacataga cttcgattcg ctgaaggaat
     2521 cgggttacag gctgatcctc ttcgacttcg acaacaccct ggaagactgg ggcgccagta
     2581 ccgtctccag ggaaaagaag cttctgctca gcaggataga gaggctgggg atgagcgtcg
     2641 tacttgtttc aaacggaaag aaaggccgcc ttggcaagat agacgacgag cttgcgacga
     2701 taaaggttct cagcagggcc agaaaaccgt tgactttcaa ggcgaagaaa gttttgaaag
     2761 atctggagat tcctccctac cagacgatcg tggtcggaga tcagctgttc acggatataa
     2821 tcatgggcaa ccttcttgga gcctacacca taaagaccga acctctcagc gagagagagt
     2881 ttttctggac cagactggtc aggagagtgg aaaggatgct tctcaaaaac atgaagaaac
     2941 accctagcgt cgaggctatg gacagatgaa gtgcaaaggg tgcggtgttg aactccagac
     3001 ggtcgatgaa accggtccgg gctacatatc cgaaaaggtt ctgaacgaaa ggttgagtga
     3061 tggcaggccg gtactctgca aacgctgttt ctccatgaaa cattacggca gactcgatgc
     3121 ggagaacaat ctcgttcact cgctagagaa tttgaaggcg tatttgaaaa caaccggaga
     3181 catactgtac gtgatggatc tgtcggactt caacgggacg tttcgcggag atattctctc
     3241 gctcctgacc gatcacaggg tacattacgt actgaataaa gttgacctcc tacccctcga
     3301 ggtgaagatc gaagagataa aaatctgggc catcgacatg ttgaaggtac ccgagaggag
     3361 ggtgagactc gcctcgtccc tgaacggata cgggatcaac tctctctacg cttatctgaa
     3421 agagaacggg gagaattaca ccgccgtcgg tgtgaccaac gtgggcaaat cctcgctgct
     3481 gaatgctctc acccgctcga gcgagataac ggtgagccgt ttccccggaa cgaccctgga
     3541 agtcactcca caacgcctgg agagggccgg aataaccgtc tacgataccc ccggcgtttt
     3601 tacgagcgac agggtgagcg atcttttgag cgtctccgac cagagcgttc ttctcccgcg
     3661 caagaagctt gaacgctaca cgatgcagtt tcacagcgag cggacggtct tcgctggcgg
     3721 tttcgtaagg atcgacgcgg tcaacaacag cgatccggtc ggtatattcc atctcttcat
     3781 gccggaaagc gtctcgatcc acgaaactaa ctcctctatc gccgatgcac agtgggactc
     3841 gtggttcggg agtttcctga agccaccctt caccggttcg gcgaggtccg cgctgacatg
     3901 gaagagagag cgatactctc tgaaaactgg ccaggagtta cacgtgaatg gtctggggtg
     3961 gataaatata gcaaaaggtc ccgtgagttt atctgtaacg ttaccggaaa gtgttagatt
     4021 aacagttaga aagggcctgg taggtccgaa gaaattcaag agatagagaa ttctggaggt
     4081 gtttcagtga aattaaaaga gcccgcaata aaggccggcg tttcgaatcg acatatccat
     4141 ctatgtgcgg aggacatcga gatcctcttc gggaagggtc accagttgac tccgatcaaa
     4201 gatctgggcc agcccgggca gttcgcctgt gatgaaaaag taataatagt gggaccgaag
     4261 ggttcattga caggaatcag agtgctggga ccggcgagaa aggcgacaca gatcgaagtg
     4321 tcgagaaccg atgccttcgc ccttggtata aaacctccca taaaggattc cggcgaccac
     4381 gaaaacactg tgggactgac cgtcgtcgga cctaagggga cgatcgtgtt gaagtccggg
     4441 gttatgctgg ccaagaggca cattcacatg actccggaag attccctggt ttacggtgtc
     4501 aaggacaaag atatagtgat ggtctactgt aagggtgacg gtaccagaaa gacgatcttc
     4561 gatgacgttt tgataagggt tcacccgacg tacgccctcg agttccatgt cgatgtcgat
     4621 gaggccaacg ccgcaatact gaacaacaac gatcctgttt acataataga ggagctttag
     4681 tgatccatat cggcacgagc ggttactcct tccccgactg gataggtacg gcctacccaa
     4741 aagggctagt gtcatcgcag atgctgaggt attaccacaa tgtatggaag ttcaacgccg
     4801 tcgagctgaa tttcacttac taccggatgc cctcttacaa aaccataacc ggcatgttga
     4861 gagagatcgc cggagagatg gtcttcgcgg tcaaagcgcc gggcaaaatc acgcacgagt
     4921 tgtggaaggc gccttcggcc gatctcatgg aactcgctgt cggttttctg ggcgcactct
     4981 ctcccatgag agaggaaggc aggcttggac cgatactctt tcaattcccc tggtctttca
     5041 agaagagtga agataatatg gcctatctgg agagattatc gaagatcttt gactccggcg
     5101 agaaccggct cgtcgttgag ttcagacacg atagctgggc tggagaagag agcttcgaat
     5161 ttctcgggaa gatcgatgcc gtcccagcgg tggttgatga accgcttata gccggcctgt
     5221 ttccttattt gcccagacag agaagaaacg ttgcgtattt ccggttccac gggaggaatc
     5281 ccggctggtt tgaaagcgag gggagcgaaa ggtacaacta cgattactcc gagaatgatc
     5341 tgagaagttt tgccctggat gtgctagaat ttcatggaca cggactggaa gtgttcgtgt
     5401 ttttcaacaa ttgtcatctg ggaagagcgg tccacaacgc actgcgtttc agagacatga
     5461 taggaggtgc ctgaattacc gatgacattc cctgattacg ttaatatggt tctcaagatg
     5521 atcgccgaag gaaacgagaa tcacgagatt ttcgaacagt tgcagaagct aggtcttagg
     5581 gaaggcgaaa tcacagaggt ttacaacgct gtcgtcggca tgattagctc aggtcctgaa
     5641 tctacagaac tgattatcga tgaactgtac gatcccagaa gaagggtatt tctggaagag
     5701 gaacttgaag ctataaaagc cgttccaggt ttgtataaag ctctagtctg gggtgagata
     5761 accccgggag attttgaaag aactctcagc gacttttcct ccggtaattg aaaaacccgc
     5821 cctcgagggc gggtttttta tgagatccac cttgccagtg gtatgaagta ccgatcggga
     5881 aggtgatcgg ggttgggaac gacccttatc gtataggcca gcttcccttc ctctggaatg
     5941 gtgaactttc caccgtagat atactcgccg tgtacatctt cctttatcag actcatggga
     6001 tagagtttga aattgatcat ctcgccgtta tcgtccattc tagcgacgaa aatctcgacg
     6061 agaacctcct tcggatccat cgttccgaga tagatgttgg ccgagagcga tacctcctgc
     6121 cctactttta tcgatctggt cgtggactcg acctgtatgt tcggctttat cctgatagcg
     6181 ttccagctcg cgctgaactt ttctttccat cccgagacgt tcttcgcaag tctgaagtcg
     6241 ttcttttgaa cttcgtcgaa gagatcgatc accgggaagt atagtttctc cgcgtaatcg
     6301 gccagcattc tctgcgtatt gaacctcttg ccaatctcct ttatcgagtc cttcattctc
     6361 tgggcccatt ccacagccgt tcctttctca tccctcgagt agtagagcgg aacgatctcc
     6421 ttctcgagag cgctgtatat agaaacggaa tctatgcggt cctggagttc gtggtcctgg
     6481 taatccctgt tgtcaccgat agcccagccg ttgtttccat cgtaaccttc cacccaccag
     6541 ccatccatta cactgaagtt gagaacgccg ttcatgcctg ccttctctcc gctcgttccg
     6601 ctggcttcgt ggggtctccg gggtgtgttc agccatatat ccactccgct gaccagatgg
     6661 cgcgcgatgt tcatatcgta gttctcgacg atgatgattc tgttcttgaa ttcgggggtc
     6721 ctggagatct cgtacagctt tcttatgagc tccttgccgg gatcgtccgc cgggtaggcg
     6781 ttgccggcgg aaacggactg aaccggcggc tcggagctgg agataataga cttcagcctg
     6841 ttgacgtcct tgaatatgag ggttgccctc ttgtaggttg cgaaccgcct ggcgaaacca
     6901 atggtgagaa cgtcctcggg aagtatttcg tcgacttcca tcagatcttc tatagtctct
     6961 cctaaccttc ttcgctgtct cttcagcctt gtttgaagga aactcttagt ccgtgatttc
     7021 aaagccaggt gaagctgcca gatttcttcg ggcggtatat cgtccaccct ttcccagacc
     7081 tccgggtcgt ccaccctttc gtgccagtcg tcaggcagat aacggtcgag tagcttgacc
     7141 agcgtagagt tcaaccaggt ccatatatgc acgccgttgg tcacgtgagt tatgggaact
     7201 tctacggccg gcaggtcagg atagatatga ttccacaggt tccgactaac cttcccgtgg
     7261 agttcactga ctccgttgga ggccgtcgag agttttaggg cgagaaccgt catgctgtaa
     7321 tgctggccac cgttggggaa cttttccagt ccgagttcca tgaactcttc ccggttggtc
     7381 ttcatcttct cccagtagcc tctgaaatac cgctcgattg tctccaggtc gaaggcgtcg
     7441 ttaccggctg gaacgggtgt gtgcgttgtg aagacgtttc cagacttcac cagtgtcacg
     7501 gccgtggcga agtccagccc tttcccctgg acgtactccc tgatcctctc cagagagaga
     7561 aaggcggcat gtccctcgtt catgtgccag agctcggggt tgatccccag tactctcaga
     7621 gccctcacac caccgattcc cagaagtatc tcctgcttga gacgcatctc tctgtcgcct
     7681 ccgtagaggt tggaggttat cttcctgtct tctgcctcgt tctgcggaat atccgtatcc
     7741 agcagaaaga gggcagtctt tccgacctgc accctccaga tcttggccca caacttccta
     7801 ccgggcagat cgatgtttat ataaacctcg tctccatttt catccactgc gggcacgacc
     7861 ggaaagtcct cgaagtcgta gcgactgtat atgttctgct gccagccgga ttcgtcgatt
     7921 ctctgctcga aatagccgtt cttgtaaaga agaccgacgg cgacgaagtt gagtcccatg
     7981 tcgctggccg tcttcaggtg atcgcccgcc agtattccca atccgcccga atatatgggg
     8041 aaggattcgt gaaaaccgta ctcggcgcag aaataggcca cgcatctctc cctgcgatcg
     8101 ggatggttcc tctcgagcca ggtgttttcg ttgcccatgt attggtcgaa gtttttgaga
     8161 gtgttctcgt acatagccag gaactgtctg tcctcttcaa gttcgtacag tctctcctgt
     8221 tttatcctcc tgagcaaacg gacgggactc cgtttctcct ggtaccacat ttccggatcc
     8281 atgtatttga acatctcctg cgcatcgtaa ttccatgacc accagagatt tctggccagc
     8341 tcttccagtc tggccagttt ctccggtaat ctgggaacgg ccataactct ttcgagaaag
     8401 tacatatgat caacctccaa ttaacagctt ctacttccaa ttataagtca ttttaggcga
     8461 tcctgctttt ataactcttt ttatctccat cttgctctcg attgtggcct taaagatcta
     8521 ctatcaatag aaagaaggga gctctgctcc cttcctctat ctcatatctc ataaaagtct
     8581 ctcagggtct tttacggata cctctcttct tgtcgagtct tctcttaaag gcggaaatct
     8641 tctcgccgct atctcgcatg aatttcgcca gtttcttctc gaagtcttga ttcgagggtc
     8701 cttcatcgga tcccttgctc ttcagatctt tgatcgaaag ttcccacttc ccgtccttcg
     8761 ttgtccccag aaccttcgct tcgactttct ggccaacctt gagatagtct tcaatgttct
     8821 tcacgtactc tctcgccact ttcgaaatgt gaatgaatcc ctcttctccg ccttccagag
     8881 taacataagc tccgaacttc tttacagaag ccacacttcc ctcaacaacg gtacccactt
     8941 tcacgctcac atgactaacc tccatttgac tctatactag agaagggcgc ctggcgccgg
     9001 ctctcagtaa ttgtctccgt atttgtttct gaacttctgt acccttccct cggtgtcgat
     9061 gatcatcgaa gatgcgtcac cgcggtagaa gggatggcaa ctcgagcaaa ggtcaatctt
     9121 gatgttatcc ttagtggagt agaacttatg ttctgcaccg caagtacatt tgactgagat
     9181 cagcttcatc tccgggtgca ttcctttttt catcggggct tcacctcttt aagttgtaat
     9241 ttgcacagaa ctcattatat catagagagg aaagtgtgtc aaagacttga agatggccat
     9301 attctgaata gaggagtttt ttgcttgaat gcagcgataa gatcgagaat aatagataac
     9361 gtctcgtcgg aaggtttcta ctctttctac gggaagcgga aagacagttt ggagagatat
     9421 gcaaagtttc taaaaaagaa tcccctggaa agaactgttc tcggaaagtt gaagaggatt
     9481 atccctgaac tctccggtct ctccttcgaa gagctggagt tcaccataga catcctgcga
     9541 gagagggatc gctcgcctct ggaaagagta gagtatgtga gcggacttat gaacctgccg
     9601 gcgcgaccgg tcggacacct ccttttcata gtagatccga ggaacaaccc gccggtcaac
     9661 ggccttttga agggtgaagt ggagagtctt gaggattacg ccagatggat cgaagaaacg
     9721 ggttcgctcc aggagatggg cgtgatgaat tacatcatgc tggagtcggc cctctgcttc
     9781 aagaaagaaa ccagcgaaga actcgatatc gacgtgcgga taaaggcgac ggatttcacc
     9841 aatctcaaag aactcagaac gctgagagaa gaagtacagt ctctcgacag ggagcttttg
     9901 aagaggctga cgcgtgagct taagtccgtt cacccttacg tcaggtcggt tctcttttct
     9961 cgctctcaca gacaggtagt gatagacgga agcaatatag tctattccag gcaggacgct
    10021 cccgatctgg cccggctgga cgacctcttc gtgaacatgg cgaaatcgcg ggtcgcactc
    10081 tttccctttc gcgtggtctt cgatgggaac atcgcttaca ccataggcgg tttccagcag
    10141 gaaaggctcg ccagatggct ttcgctgccg caggttgaga cttactcgcc ggccgacgag
    10201 aagatcatac gtctcgccag acagcacgac gccgtggtga taacctacga caggtacctt
    10261 gaacacggcg ccggagatct gatattagtc agaccggagg agatagatga aaacctcgga
    10321 ctttgattac tacctccccg aagagctgat agcgcaaaaa cctgcggtac cccgcgacag
    10381 ctcaaggctg ctggttatcg acagagcccg tgaatcgttc gatcacagat cgttcaggga
    10441 gataatcgag tatctcaacc ccggcgatac gctcgtgatg aacgacagca gggttattcc
    10501 ggccaggttg atcggccgca aggataccgg agcctccgcc gaggcagtcc tcatcgaaaa
    10561 aatcgaccgg ggattgtgga aggccatagt caggccggga aacaaagtga agaagggcag
    10621 caggttgatc ttcggcagtc tggactgcga agttgtagag cactgtgacg attccagcag
    10681 gctgctggat ttcaagggtg ccagggacga cgagataaaa ctcgtcggca ggctggccat
    10741 acccccgtac attcacacct atccggagga cccggaatcc tatcagacgg tttattcgaa
    10801 gagagagggg tcgatagcct ctccgaccgc cggactccac ttcacgtccg aactgctcga
    10861 gattataagc gagaagggtg tgggaataga gtacgtaacg ctacacgtcg gaatcgggac
    10921 cttcagaccg gtgaaaagcg agaggatcga agaacatcaa atgcacgaag agtattacac
    10981 actatcgtcc gaaaccgtcg aggccgtgaa ccgtacaaga gaaaggaacg ggagagtggt
    11041 ggccgtcgga actaccacgg tcaggacgct cgaaagcgtc gtatccaggc acggaagact
    11101 ggtggaagac agcggttcga cggacatctt tatctatcct ccctacaagt tcggcgtgat
    11161 agactcgcta ataacgaact tccatctgcc gaaatcgaca ctacttatgc tcgtgtcggc
    11221 gtttgccgga agggaactga tcatggaagc ctacagggag gccgtcgaaa aacgttacag
    11281 attcttctcc ttcggagatg cctgcctgat tctgtgatag agcttatctg aagcttataa
    11341 aagtgttccg tcaaatcacc tggctgccag ataagcttct cagtctcaac atctgtatgg
    11401 taagaaaggg cggaaggttg tcacactttt tttgcccgtg agatatctaa ttccaaatta
    11461 aagcaacaga aaacgtctac aaatcgtata ttgaacaagt ccattgcgtg attcgcaaaa
    11521 ctccacggta tggaaataaa ctatgcggtg agtaatatcg acttttccta catgctcaaa
    11581 aggctaggag gcaccatccg ctgactactc ctcatccagc agcggcgata cgaaatgtat
    11641 ccagagcatt tttatcagta tcgcgaaggg gacggcgatt atggccccga acagtccgaa
    11701 gagctgtcct gcgacgacta tcatcagtat tatcatgacc ggatttatcc tgttgtagcc
    11761 cttcatgaat accaggaaga gtatgaaggc cgccgcgtga acgatcgtca agacgatgag
    11821 aagtatcaag accagcacga tgttctgtgt tgaaaccgcc agtagtaaga gcggtatgaa
    11881 ctccaggggt acccccacga tcggtatgag gttggtgaaa gcggcccaca aacccatcaa
    11941 cagactgtat ttcagtccca tcgccttgat tgctattccc accacgatgc ccactatgat
    12001 cgcaaccaga acgatggaga tgacgtacct ttccaggttt ttgtaagtat cggagagaaa
    12061 ttcagtcgct atcttcctga ctttcttcgg gtagagtttg ggtactatct cctttatcga
    12121 gagaaagtag taacttagat agatcgcccc cactatggtg aagaaaatca ccagccccgt
    12181 acttgtgatg aaactgggaa ccttttccag aaggcttggc agaaactctc ttaggatgtt
    12241 ctgtacctga atcaagaagt ttctgacgct ttcatacgtg gccgcctgcc tggccggatc
    12301 gaggttctta ggcatgaaat aggctaccac ttcgcgccac agttcctgcg actgaacctg
    12361 catcgcattc gccagaagct cctgcgataa cattgccgca ttcgacggag agagatatct
    12421 ggaagtcaca tcgttcaact cttcgtagct agcctctctg gtctggtcgc tgctcatgat
    12481 tactatgtag tttccctttt ccctgagact ctcttctatg ctcttcaact ctttctcgaa
    12541 gggggtctcc atcgtgggaa ctgtcttcat tatctcggcg tagtagctct ttagatcctt
    12601 tgaaaggaac tcaagagagc ccttctcttc ctgtgtcaaa ccggtcgcga aattttcgtc
    12661 cgagaggagc ctgtctatgt tggtgttcac ttcgaaggag gttatcttgg tctcttcgac
    12721 cgacgggaag aaatcgacga cgatgttgta gaacttcgac gcttcctttg ccagcggggt
    12781 cgtcaagaag acaataagcg ttataagccc tacgaagatt atcaccagcc cgaaaccgag
    12841 ggagagtgac cgctttattt tgaagtattt ctgagcgagt ctggtccagg gttcgagaaa
    12901 gagaaccacc gtgaaagtcg agaagactat gacggccgtt ctgagtgaaa tagagaagac
    12961 tacaagaccg atgatcaagt atatcaaagt gaaaacggct gcgatgaagc gcggttgttt
    13021 tatgatgttt cgaagcacgt caagccccct tcaaaggaag acggaaaagt ctctcgatct
    13081 cttcccggtt gtccgtaatt cccatcacga tcataagttt tatcctggcc ttcatggaag
    13141 acagttcctg cgcgaatata gccccgatat cggcgagact ttttccgccg ccgatataac
    13201 cgtaaactcc tagaacacga cctttgaagc atctagaggt tataattacc ggcatacccc
    13261 tttcctctat cagatatttt actacgtgat agacctcaac cggcacgtta ccccttccaa
    13321 aactttctag gacgacgcct ttatatccca gttctggcag agatttcaat atcgagccat
    13381 cgtctccggt gaaggttttg accagagcca ctctgttttc tatcctttcc acattgaaag
    13441 tggccctcag aagaggtttc ctgaaaaaga tgaccgtgtc ctcatccact atccccagcg
    13501 gtccgtgacc gggtgagtcg aaagtggcca cattgctggt ataggtcttg gtgacttctc
    13561 gcgcggcatg aatctcttcg ttgagacaga cggtcgttcc catacctata gcaagagagt
    13621 tgagaacgac cctcaccgaa gccagaacgt ttctgggacc gtccgttccc atctcgtcta
    13681 tatttctcat cgccgcggtg cagacgaccg gtttctcgct attcacggtg agatccaaaa
    13741 aatacgctgt ctcctcgaga gtgtccgttc catgggttat cacgacgccc gctacatcgt
    13801 ctctgctcag ggttttaaaa acgagtctcg agagcttcca catagtatcg gggtttatgt
    13861 aaggactggg gacgtttgaa aactctatat gttcaacatc ggccagttcg tcaagacctt
    13921 ttatgttcgc cacatgcttg ttgatccctt gcgccggtat cgatgcgccg gaaaccgggt
    13981 cggttatcat ggcgatcgtt ccgcccgtcg ttatgattac cactttgccc ttcaaatatc
    14041 cttacccccc agcttaagtc cgagtttcga catcgtcttg attgtatcac ttccgactat
    14101 ctccgcaagt tcccctgtaa ccgaaggcct cttgaaaacg atcgttctgg cccgatctct
    14161 gccgatgccc ggtatgtatc tcagctccct ctcaccaagc gagtcgatat ccgttcccag
    14221 tggaagaccg gtgatcgatc tcattccgtg atcggtgacg gcaatatccg tcttcccctt
    14281 aaaagagacg ggcgatccca ccagaatcgg ataggtgccg agcggtctgc cgaagaagat
    14341 ctggccttcg ttgaaagcgg ggatcactcc tcaggcagaa gctttggaat tccgtccacc
    14401 ctttggccgc ctatttcgtt caccggctct atggctctgc gtatatcttc cacactgcca
    14461 ccgaggttgt tcagttcccg tacccgtgtg tcgaagcttt ccacgccgaa ggagatcgta
    14521 tcaccacagg tgtcgtacct gactatgctc tcgagagcct ttcttgtctc cctttcatga
    14581 cgggcgatga agatcggatt accgttgtct atatgcaata cgtcaggatc gagtacctct
    14641 ctgactgaaa tccgggttga aagccgttgt accacccctg agcgtgtatc ctgccgaaac
    14701 tatgggaccg gccactattc tcaccggtcc gtcgcaaagg ttgagaagtc gtgagatctc
    14761 cgtgctggaa atcggagtgc cccccagata ccttccaggt gtggttatac ctccgtggat
    14821 gaccagataa tcgtagtcta ggcatctgtt ccactgatct ctctccctca ccaagtctat
    14881 tgtaagatag tcaacatcga accctttcag caaaaaggcg ccggcaacga cttttatcct
    14941 tggcgaaatg taaggcggca caccgaaggc ggccggttca tctatataac catcaacgat
    15001 caaggctttc atagaggttc ctccgtgatg cgtgcatttc tattacagtg tataataaaa
    15061 acagtaaata ttgggaggga aagtgttgcc gttcgaaaga cattgttcat tctgtggaag
    15121 accgtcctcg caggtcaaga aactcatatc gggaacggaa ggttatatct gcaacgaatg
    15181 tgtcgatcta ttccacgaca taatacagca ggatcaaaaa gccaaaaaag gaccggggaa
    15241 aaagctaccg actccggccg atatcaagat ggaactggac aaatatgtta tcggtcagga
    15301 aaaggccaag aagataatct ccgtttcggt gtacaaccat tacaagagag tcttcaacga
    15361 tgttgaggac gttgagatag agaaatcgaa catcatgatg gttggaccca ccggatcggg
    15421 aaagactttg attgcccgaa tcctggcccg gatactcgac gttccatttg cgatagccga
    15481 cgccacgccg ctgaccgaag ccggttatgt gggagaggac gtcgaaaaca tagttttgag
    15541 actcctccag tcttcgaact tcgacgtcga aaaggcccag ctgggaataa tatacataga
    15601 cgaaatagac aagatcgcca ggaagtctcc caatccatct atcaccagag atgtatcggg
    15661 cgaaggcgtt cagcaggctt tactcaagat agtcgaaggc acggtggcca acgttccgcc
    15721 gcagggagga aggaaacatc cctatcagga gttcataaaa gtcgacacca gcaacatcct
    15781 gttcattgtg ggaggtgcct tcgacgggct ggacagcata ataaagcagc gtgttctcga
    15841 ctcgtcgatg ggcttcggcg ccgaggtgag gagcaagaac caattgaggc tcggccagat
    15901 acttcaggaa gccgttcccg acgatctgat acagtacggc cttatccccg agttcgtggg
    15961 ccgtttcccg gttctggcga ccctgaacga cctggacgcg gaagacttga aaaggatcat
    16021 gagcgagcca cgaaacgcca tactcaagca gtaccagaaa cttctcggac ttgacggagt
    16081 ggaacttgag ttcaccgatg cggccatgac ggccatcgcc agcaaagcca tgaagagggg
    16141 aactggcgcg cgcgccctca agagcgtcat cgagcaggtg atgctggata taatgtacga
    16201 catccccgcc atgaccaacg tcgaaaaggt ggtcataaac gaggacgtca taatcaaaaa
    16261 cgcctctcca aagataatct cgagagaatc ggcctgatga aaatccttgc catagagagt
    16321 tcctgtgatg aaacggccgt ggccatcgtt gaagatggcc gcgttctttc atcgctggta
    16381 tcttcgcaga tagacgtcca caaaaaatac ggtggcgtcg tgccggagat agcttccaga
    16441 aagcatctgg agatagtcga tcgtctggtc ctccaggccg tggaagagac aggcctgact
    16501 cttgaagaaa tagacgtttt cgccgcaacg atgggaccgg gtcttgtcgg atcgctgttg
    16561 ataggccttt cagtggccaa gggactggcc atctcgttga acaggccatt cataggcgtg
    16621 aaccaccttt acggtcacgt ttacgccaac ctcatctcgt gtcccgatct caggccgcct
    16681 ttcatggtgc tcctggtttc cggagggcat accgagatac tgctcatgga ggaatggaac
    16741 acagttgaaa tggtcggaag gacgcgtgac gatgccgccg gggaggcctt cgataagata
    16801 gccagactgc tgggtcttgg atatccgggt ggacccgaga tagagaaagc cgccagaggc
    16861 ggctcacccg tttacagctt tccccgtccc ttgcaggaga agggaaacta cgatttttcc
    16921 ttcagcggtc ttaaaacgtc tgttctctac ttcctccaga ggaatcccgg agtttcggtc
    16981 gaagatgtag ccgcttcggc gcaggaagcc atagtcgatt cgctggtgac taaaaccttc
    17041 gacgccgcgc agagcctggc cgtgaataag atcgccttgg ccggtggggt ggcctccaac
    17101 tctcttctca gagagagagc cattgatatg gcttctaaat gtggaatacg ggtcttcttt
    17161 cctccgatcg gcatgtgcac tgacaacgcg gcgatgatag ctgtcgcggc ctacgaaaaa
    17221 gcgaagaggg gactttactc tcccctctcc accaatgctg ttccatatct gtctctagat
    17281 gtcttctgat cagtcttttt tcgaaggaac cgttgaagtg ctgctcgaac tcgatgtttt
    17341 cttggaatcg gtcgaataat atccgccgcc cttgaagact attcccacac tgctcagcag
    17401 tttcttgagg tgtcccccgc acgatggaca gacctttaac ggctcgtcgg acattttctg
    17461 gaaggcttcc gttgtcttac cacaatcctc gcattcatac ctgtaaaacg gcatggctgg
    17521 tccctccttg tgtctcaaat tacttcttta tgtattaata ttctattcct cgtcggccgt
    17581 atttggaata acacagataa actcgaggtt accttttccg ctattcttga actggtgaac
    17641 cgcattggcg ggaacgaaaa catagctgca cgcctgtgcc ggttgttctc cttcctgtgt
    17701 gacaaccgtc gcattccccg atacgatata aacctcgtgt tcccacggat gactgtggag
    17761 aggactgtag ccgccctctt cgacagtgaa gagcctcatc acgaaattct tcgccccttt
    17821 tgacttgcct atgagcactc tcttctgtac ttttttgacc ttttcgttgt ctatgagctg
    17881 gggttttacc tcatgcgctt tgcctacttg gatgctgtcc ggcatctccg tatcccctcc
    17941 ttctgaccgg gtcgcttttt ctacgatcgt ctctgtattt tcgttcactg tattcgacaa
    18001 ccacttttct gtaaggttca aaaccgactg aataactctt caaatcatgg tactgggata
    18061 ttacttcgtg aaccagtctc ctctcgaaag agaacatggg ttccataact atcttgccct
    18121 tcttcgacag ggccgacttg gccgttctgt gggctatctc ttcgacgagt ttctttctct
    18181 cgtaacggta atcgcccaca tcgacggtga cgttgacctt tatctgtgcc attctattag
    18241 cgtagatatt tagaatatgc tgcagggcgg ccacggtctt cccgtgcttg ccaatcagcc
    18301 tggagatctt ctcgccctct atttttaccc tgtaggttct tccaaccagc ttgacttccg
    18361 agagtacttc ttcgtcgaag ttgccgagaa tacccttcaa atactcgtac agtttccttt
    18421 ccgaatgact atcgtgaaga gagacttcaa ttactgactc tttcgtgccg aagattccga
    18481 ggaagccttt ggaacccttt tcgattatct ttatatccag ttcgtcttcg taagcgtcca
    18541 gttccctgcg ggccagtcgg atcgcttcct cgacagaatc ggccttgaat tggtacgatt
    18601 tcacattaac acctccatga gagcaactga atactatttc gccggttttt caggcagtcc
    18661 cagcatctgc ctcaacgtga gaccctttat cccgtggcgc ttgtaaacca cccagcttat
    18721 gagaagctga agaacgctct gggtagtcca gtaaagaagc agaccagatg ggaaggtcgc
    18781 aaagaggatc gggaagatga gttgcataat cacgcccgat ctggcagaac gtgcgtcttg
    18841 tgccgttagg agcgagctga acacaccgac cacgatggta atgattatga acgggatgtt
    18901 ctggatgaaa tcaccgacgg acagatcgct ccatataagg aagcctggcg tgtaagccat
    18961 gacttcgctg aagtagcgta tggcgttgta tagaattatc aggatcggca actgaatcag
    19021 aatcggaaaa caaccggaag cgggattgta tcctttttcc ttgtagaggg ccatcatggc
    19081 ctcctgttgt ctcttgggat ctttgtactt gttttttatc ttttcgattt ccggctgaag
    19141 cattctcatc ataagcatgg acttcgtctg tatgtggtaa agcggataga gaacgaatct
    19201 ggtggcaatc gtgaaaagta tcagtgccca gccgaagtta cccgaccact gatacagcca
    19261 gaagagaaag taaactatac cgtgatagat ccaggagaag aaattgactg ccccgaaacc
    19321 ttcgaggtcc ttgacgagag cgctgtaata ttcggggaga gcctccttga cgtgaatcaa
    19381 tctcatgggg ccggcgaaaa cctcgagaat ccccttgcca ctatcgctgg catagcgctg
    19441 gaacttaaag gagccttgct gcgaggcgat ggcgaagagc gtcttctgac cgctgaattt
    19501 gtcataggtc aaatagtaac tcgaatcggg accgagcgtc tggttgttgt aagatatgaa
    19561 ggggagggcg acctcgacgc tttggttaaa atctactacg acgttgaaat cgtagtacgg
    19621 gctgttgagt ataacaaagc ttttccggcc ctgctggtag aagaaatcca ctactacgtt
    19681 tccttccgcg tcggttgtgc gcgagaacga ctgaggcaga agctcgttgc cctgctgatc
    19741 gaagatgttg aagccatctt ttcccagact ttcgttgtat gcgtagaact cgaccccttt
    19801 gttgtctgcg aagctggtta cgcctgtgag cagccccttt tcgatgtcga tgactattct
    19861 aagcgtcttt ccagacacaa tgagagtgtt ggttccctct tgagtaatca ggtctgcgcc
    19921 catgagcgaa agtgtgaaaa cggagagaag caggacaagc attatcaatg tttttctcat
    19981 ctttcgtgcc tcctaattaa tgtaaaatgg tctggaactt cgtcaatccc gccgggattg
    20041 aaggggttgc aacgcaaaat tctccatgtg ccgagtataa ggcccttgaa agctccgaac
    20101 ctctcgacag cctcgtaagt gtacaaagaa cacgtgggat agaacctgca tcgggggcgg
    20161 gtccttggag agatcttctt acggtagaaa tctatcagga aaagtacaat ctttttcatc
    20221 gggcatttct ccaattttgt tagcgattct tctcaaaacc ctttgaatct caaaaaacga
    20281 aacttctctt cgtctaaaca ggtctgataa atcctttctt gcaatgaaga gtatatcata
    20341 accctcgggg aagtcttctt ttcccgtccg gtaaacctcc ttaacgagtc gtctgagcct
    20401 atttctcaaa gtcgccttgc cgaacttgcg cttaaccgat acgccgatac gagaaaaagg
    20461 cagactattc tgaacaaata aggcaacaaa aaaagggtca actatcgatt ttcccttttt
    20521 gaaaaccctg tcaaagtctg ccttccttct tataatctct gtttttttca gggactgatc
    20581 ctctgatttc acacagcgag tctctttctt ccctttcttc ttctgctggc gagaaccttt
    20641 ctaccgccga ccgtacggga cctcacaaga aaaccgtgag tcctgtttct cttgaccttc
    20701 gatggctgat atgtcctttt cataataaca ttacctccag aataaacatc acagtgaaca
    20761 ttatacggag tggcaaccgt actgtcaagg cagaaaagat aacaattgct ttccggtgta
    20821 gagaacgcca tcattatcca tcgactgact ccacctcaag ttagaaggaa ggagatgaag
    20881 ataaaaaatt aatccgcatt ttaacagtcg gggtcttcgt aacatctctt tgggtctctt
    20941 gtttcgaaat tcgagttttt cagctggtaa aattgacagc aattgagcaa acgattttct
    21001 actgaaatga gggaggtttt tttgttgaaa gacaccgtcg cttttcaggg ggaacatggt
    21061 gcttattcag aacaggccat aagaaagctt ttaggagaat cgcccgtttc tctcccgtgc
    21121 agatcgttcc aggagatgct gtattttgtg attgaaagaa aggcagacta cgctattctg
    21181 ccggtggaaa actcgttggc cggtacagta attcctgctt acgacgctct tatagatagt
    21241 gatcttcttg tccaggccga agttatgctt cgaattgagc actgcctcat ggctcctaag
    21301 gggaggtcga ttgaggatat aaaatacgtt atttctcatc accaggctct ggcacagtgt
    21361 tcaaaacata tagaagaaga gggttttgag gcgaaggaat actatgacac tgccggtgcg
    21421 gcaagagacc tggcaacttc ggagatgcca ttcacagcag caatagcaag tgaactcgcg
    21481 gcaaaaacct acgggttgca tgttttgagg agaaatttcg aagacatgga aaccaataca
    21541 acgcggttct tcttgatggg aagaggagct gtgaaatgta ctgaaggatg taaaacctcg
    21601 ataatgttca ctacggaaca tctaccaggc gccttgttca gagtccttgg agagttgtcg
    21661 aatcgcaatc tgaatcttac aaagatagag tcaaggcctt ttaaaaggga gatgtggcac
    21721 tatctcttct tcgttgattt cgaaggaact gttgctacaa gaaaagtaga agaggctatc
    21781 gaatcaatca gaagcctctg cagatacttc aagtttctgg gctcatatcg ctctgccggt
    21841 tctgaaacat gaattcactc ctcggccgca tttcgagtta aagaaatgcc tgagttgaaa
    21901 ctccggcaaa aaacaaatat accgctgcag tagaaaacgc tgatcggtac cgttatcgct
    21961 gccattttgc tcacggcctt tcttaattct tggggataaa gcaatgcaac gacctgcgcc
    22021 tcttgacaca caggatagta taatatcagt gatctctgtt ggccggatta gccgattttc
    22081 ggaaatgggg gaatgtgatg ttactttcgc tggcaggaag gaatttcttc acgttcaggg
    22141 agttctatgt cgagttctcc agtgggatga acgccattac gggagaatcc ggcgccggga
    22201 agaccatctt cctgaaggcg ctgtggactg ccgtcggatt ccccccaccc tgggccggtg
    22261 aagagtccgg aaacatcgag gccaatttct ccatagaaga ttcgattctg aggaggattg
    22321 gggagttcgg aacagagatc ggagacggta atcaactgtt ggtgaacgtc aatttcaccg
    22381 gccagagaac tatatacaga ttgaacggca gaatggtgcc caggcagatg atccag
//
LOCUS       DAOOLY010000006        22333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_2241_length_22333_cov_5.530568, whole genome shotgun sequence.
ACCESSION   DAOOLY010000006 DAOOLY010000000
VERSION     DAOOLY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 22333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22333
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2241_length_22333_cov_5.530568"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..2437
                     /locus_tag="PLD15_00665"
     CDS             545..2437
                     /locus_tag="PLD15_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB62436.1"
                     /translation="MKGNFFPDSRYDVAIIGSGLGSLAAASLLANRGLKVLVAERHYL
                     PGGCCSIFRRNDFTFDSAVGMVFGFGNRGFNSHRFVFNEIGEDIDVIRHEALYSVTFG
                     EKRIIFWPDLDRFISELGRHFPGYEDQLRKFYDYLADFYHNVIVADPVIVPPTEMKRS
                     DSLKALLKHPVRQARMIRLLFRSTEELMSKFIDPKAEELYQFFDVLTSTYCYTTVKET
                     PGVLAVTMFIDNHEGGGFYPSGSSQMIPNKLEKSIERNGGYMLYENRVDEILFEDGKA
                     AGIKLEDGSEIGSNYVIYGGTVWNLYGGIIPKERIGPEKLKWVESLEPTFPAIVVYAA
                     VDYSAVPIGTNPVEMFVEEFDEIAESDVTVYISSIDDPSICPEGTHVMSMIAPSKKKW
                     PPRGTEQYKEMKKAETERVLKLVEKKFPKLRDSLKHLETATPLTIERYTLKNEGRVGG
                     PKQSIGQELLKRLHARSEWKNLFICGDSTVMGMGTPAVTVSGIGAANLILRDLRMKEY
                     SYREPEVERVHYLSEKKRKAVDLEKTALDRDTVPLISGSCQLCEIPGCKMKCPDGKDI
                     RGVLRRLESGNFPGAKRRLLETKVSTDCADCPGYCEKSCRRLSFDDRPVPIKRLLSWL
                     EKEVII"
     gene            2434..3969
                     /locus_tag="PLD15_00670"
     CDS             2434..3969
                     /locus_tag="PLD15_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB62437.1"
                     /translation="MKFDCIVIGSGIAGLSCAAFIAKAGKQVILIERAEKPGGCFSSF
                     EYNGYHFDSGIKAVENAGLLFPMLENFGIMGKLNFVKNRVYAGFEDKVFSMESDESLL
                     EYFDFLGERFPESRSGIEDLLKEMQELNSFMGSLSGMSGPLYGKTTFLTKLKSIPWFL
                     KNGKKLLGFLKKLKYNNIPFRDFLKVRIPDDELVSIIDEPFFEGTPAFFGLAYPTVFR
                     DYFYPEGGIERIPLTLADFIRENGGTVLTGTEVTGILLERGLPSGVVTDTGQEIESPI
                     IVNAADAKRLYNKLLPENSVDQDFLQRLNRAQVSDSVFSVFMGVDTAPENLDQHVEHI
                     IYVPAAAKPCDYESPEYFKTTGMEISIPCMRDPSLAPAGKTGVILNVNTTQKALNGWG
                     RSVSRAEYERLKEQAADDVMANFFRLYPSLREKIEFRIVATPFTMEEKTLNSGGSITG
                     WNYHPSETFPLRDFRKMKRSIDTPVKNLFQIGQWSFDPAGVPVCLITAKLAADRVLNI
                     SRP"
     gene            4049..5368
                     /locus_tag="PLD15_00675"
     CDS             4049..5368
                     /locus_tag="PLD15_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HPB62438.1"
                     /translation="MKKALIKALKSAKVEFVRSLWCDNANVIRAKSIDIDLLEDNFET
                     VAGISRAQQGVPVMYDGVVPESGLMPVGEIWLNPDWDTLNVLPYTPGHASVISDLFAG
                     RYEWSLCPRFFLKRAIKALDELGFTMRAAFENEFYLFDRSLNPVDNTLFASSFSMDLN
                     SKVVGDIVRNLKAQFLNVERYYAESGPGQQEIVIAHAPALEAADNQITFRETVRTTAV
                     RNGLMASFMPKPFEEFGGSGCHLHFSLLKDGKNVMRDHNGELSEVALYFMAGLLEHLP
                     SLMAVTTPTTNSYRRIKPSCWAGAYRCWGLDNREAALRVPTDPSGSVAGHVELKSVDA
                     SSNPYLALGMMIFAGIDGLKKKVLPPVNLQGDPALMNGEELLQGMVEPLPSSPGIALD
                     MLENDRYLLKVLGEDLSRAYLAVKRAEWKAMGELTIAQERQILLERF"
     gene            5368..6501
                     /locus_tag="PLD15_00680"
     CDS             5368..6501
                     /locus_tag="PLD15_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB62439.1"
                     /translation="MGLDLSSLPVFDGHAHNLLLPAWLKEYPIEGIFTQSTDREYLNS
                     FAGETLAFKRSLKDIAAVLECDPSIEAIRNRREKMEQTELAGLYFERANISGVVLDDG
                     FWFHRTRPLEWHSSFASTYRALRIESLAEALLEDSTSFDSFLSDFRDALLNPPGGVVA
                     YKSIIAYRSGLRIGVHDKRAARDGYLVEKERNTGRLTDGRLLGFLFNLTLEVCRGRGL
                     PLQIHTGWGDSDLDLRESNPLHLKPLLDEDKYSRTRLVLLHCYPYAREAAYLAAIYPE
                     VYVDFGLSVPLLSVAGMEAVIGEMLEIAPVTKICYSSDARFMPEGFYLAATWARRALG
                     ESLERSVRCGELMLNEALKTAERILKENSEGLYGVARSAGGEA"
     gene            6531..7154
                     /locus_tag="PLD15_00685"
     CDS             6531..7154
                     /locus_tag="PLD15_00685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPB62440.1"
                     /translation="MLLQREQRVVFQGDSVTDTGRDRDDFFGLGNGYPAIVSGTIGSL
                     FPEMRIEFLNRGVGGDRVKDLKNRWKEDCLDLSPDWVSILVGINDCWRLYDSNDRTEP
                     ALFESNYRYLLQSAAARGASLIVMEPFYLPVYREMEMWTEDLGPKIQIIRRLAREFKA
                     VYVPLDGIFAEAATKREPSFWAEDGIHPTPEGHSLIARHWLKALGVL"
     gene            complement(7185..8717)
                     /locus_tag="PLD15_00690"
     CDS             complement(7185..8717)
                     /locus_tag="PLD15_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPB62441.1"
                     /translation="MNPFALLMFIPTVSCGLLGMYSFYKRELTGAGPLGFLMLAITWW
                     STMYGLELLSNDLQQMRLLNSISYPAIVSLPVFVFMFVMAILDRREWFTFKRVVLLFI
                     VPAIVNLTMWTNEFHWLFYTVSEIDTQWPFPVQKLVHGPLFWINIVYTYVLLVGSIVL
                     LLREWFYSERPYRTQLSIILLGMAFPILVNLNYLFKILPVGYIDLTPVAFFLTSLFAA
                     LSIFRFRLFDLRPIAKETVIRNLEDGIVVIDKSDILIDVNPVSVKLLEIDGEKALGLP
                     IDDAFSRMPALLGFIGSTDSRSEMVIGERIVESRKTSIMSKRGRLRGHIVLLTDITDR
                     KRIEEALKESQEMYRTLATTDMLTGVLNRYSLDQLLERETERSRRYREPLSVIMFDLD
                     DLKKINDTHGHHAGDTALKALSFQILSKIRKSDSLGRWGGDEFLIVAPSTDLDGALEM
                     AEKLRTEIDSGEFEAIDKLSISMGVSSLEVGEKDYDGLLRRVDRALYESKRRGKNRAT
                     AI"
     gene            8927..9697
                     /locus_tag="PLD15_00695"
     CDS             8927..9697
                     /locus_tag="PLD15_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HPB62442.1"
                     /translation="MRRSAIIVFSVLTVISTMLFSVISEDIVSIPAIIDVVSIEQRLK
                     VELVENASTGYTWSYEVSDPSFLTLVSKETAESSVDTPVIAGSPSTVTWLFSPNKSGS
                     FSLVFRLFRAWEGEETAIDVRVYNVEVQNSSLSECVPQYVTILGSSSGEIEISQLARI
                     VLQENPSTGYSWKAEVFDAAVLRTVERSLLAESDSHEATPIVELGGSQEVLGAPVNVA
                     FDFEALKAGISLVRFTYSRPWEESEQDKTLLVGIKVAR"
     gene            9886..11391
                     /gene="araA"
                     /locus_tag="PLD15_00700"
     CDS             9886..11391
                     /gene="araA"
                     /locus_tag="PLD15_00700"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPB62443.1"
                     /translation="MGVEGLEVWFITGSQELYGKEVLNKVESNSKKLVEYLNDQAGLP
                     VKIVWKPVLKESRQIRTLMNEANVDDRCIGLMAWMHTFSPGKMWIAGLKALRKPFLHF
                     HTQFDQKIPWKSIDMDYMNTNQSAHGGREFGFTATRLDIRRKVIAGHWKNPEMIARVE
                     NWIRAALGWNEMQNLKVARLGDNMRDVAVTEGNKVDAQIDLGYEVHGYAMGDFIKLVK
                     KVKESEIEALVKQYGQEYPLTRSSVSQEEFKSSLHEAAKIEIALRRFLDMGPFKAFTT
                     TFQDLYGLRQLPGLAVQRLMRDGYGFGGEGDWKTAALVRTMKVMAAGRDRGTSFVEDY
                     IYHFEPGNMKELGSHMLEVCESLADGPISLEVHPLSIGDKEAPARSVFDSREGEAVQV
                     CIVEFGDRFRMIVNEVEVVKRRQEEEMPGLPVARVLWIPKPDLPTAATAWILAGGAHH
                     SCLSYTVNTEQLEDLCEMLDIEFVVIDRDTKIRDFKRELRNGEIYYKLRKL"
     gene            11454..12170
                     /gene="araD"
                     /locus_tag="PLD15_00705"
     CDS             11454..12170
                     /gene="araD"
                     /locus_tag="PLD15_00705"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HPB62444.1"
                     /translation="MLEALKEKVFRANMKLVEAGLVILTWGNASGIDRSKGLMVIKPS
                     GVDYDELSPEKMVVVDLNGNVLEGGLRPSSDTPTHIVLYRNFEGIGGIVHTHSTWATA
                     WAQSRSPIPSLGTTHADHFHNEIPCTRLLREEETEENYEMNTGRVIVETFASKDYFST
                     PGVLVAGHAPFTWGKNLDDAVNNSVVLEEVARMAFLTKMLKSDVETLPAHIIDKHYSR
                     KHGKNAYYGQKKAPGDAPDR"
     gene            complement(12225..12473)
                     /locus_tag="PLD15_00710"
     CDS             complement(12225..12473)
                     /locus_tag="PLD15_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62445.1"
                     /translation="MRIIPETRERIKKRRMEIGILAACMLVASLYAVYQIAVNTELFL
                     SSTKVQLIMIVIFAFTVVCVVYFLILHFAYRKRRRART"
     gene            complement(12713..13165)
                     /gene="mscL"
                     /locus_tag="PLD15_00715"
     CDS             complement(12713..13165)
                     /gene="mscL"
                     /locus_tag="PLD15_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPB62446.1"
                     /translation="MWKEFKKFISRGNVIDLAVGIIIGGAFQVIVRSLVDDIIMPILG
                     LFTGGIDFSNLYINLSGGSYETLAAAKAAGAATINIGLFINAVINFLILAFVIFLIIR
                     QINKMRERQKKEEAPAAPTTKTCPFCHTSIPIEATRCPNCTSELGKKG"
     gene            13447..14034
                     /locus_tag="PLD15_00720"
     CDS             13447..14034
                     /locus_tag="PLD15_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB62447.1"
                     /translation="MAEKDLKERIKEVAVEHFNRNGYHGTTIRNIANDVGCSLPMIYY
                     YYKNKKELFDEIIKKEFFSIIKKQASLLKTSSIIDFYTRFIHDLNALNGYDKQVYRLG
                     IKVYLSFDGDDELMNLMDEWEKTILPRHYRILQPHLKNTDNDTAVVRTLVHLLETLIE
                     NIVVKNRYLPEEEIREEIAIVLRGVDGSIPDPQLH"
     gene            14118..14567
                     /locus_tag="PLD15_00725"
     CDS             14118..14567
                     /locus_tag="PLD15_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62448.1"
                     /translation="MEIEKRLAILQNTYAASVAEAVNTYDRLKELEAVVERRKERQAQ
                     TAPYMNQQLGIESVEDVFYKLSEIYGCANWKVEKTGDDYVATATSCKLCALSKKMGGA
                     NPCSGWCLDPMIAMLSAAVKIDSRHITVESTLMKSDKCKMLINTKIE"
     gene            14720..15469
                     /locus_tag="PLD15_00730"
     CDS             14720..15469
                     /locus_tag="PLD15_00730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPB62449.1"
                     /translation="MENLKLNRTLFAIGMFVVVFVTQVFLGRIGHYIASVISYGKIDP
                     YDAFAGISIHHIVQMVLALIIITVLGKLLKIDFYLKPGDVARGKKYLITFTSIFALIA
                     VALHVFMYINNQLPVYTFPLDKRNVLGTLGFQLLISGTSEEIVFRALPVTMLVYAFGK
                     SIPVKGHVTLEVILASILFSLAHTKWSLMPFKFEMDTFQIVYSFILGTIQGVVYQKSK
                     SIVYPMLMHNISNFLMVGMGYLFTALKAITS"
     gene            complement(15535..17826)
                     /locus_tag="PLD15_00735"
     CDS             complement(15535..17826)
                     /locus_tag="PLD15_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62450.1"
                     /translation="MRRILALLMIVMPVALAGINVEIDLSHLEFLRDEFDIEGSVEMG
                     YWIYADRQPDGSYRHAQASGEGVTCVDDVARVAILYLRLFENSADETHYLRAREALDF
                     LLAMQDADGDFFNFIFDDGKINRNGPTSRKGGNWWTARGFWALSTGAWILGQRDPSYS
                     IELARASQKAFRVLLNYERDGLIQGYTDVSSVALLGAVELYRISNDLEALNFIKRCSG
                     SLISKLITDDDSIFRGLVDENAPQETVPHWHGWGSRQLEALAMTALALEDDELLELTV
                     DAFKEGATLLLGAGPVYSLTGFVQLFPQIAYAAEAAISTGYTLFSITNDREIATITAL
                     LGSWFIGLNPLGQSMIGPNGEGYDGMEFSHINRNAGAESTISMLLSLERVSRLPEEYE
                     KYFSGAIELLTPAKVIEAEAMRTGLSEVDTFQGTGISGNAALKVSGVFSLRESVALPP
                     VPYSAFTGIGNVQTGPVSFTLRLGESRSEETVSIAQKSIFKVGVITGTAQESTLTLGG
                     KVESGSFDLDQLVLLPDLIALYIPGDETLVLNYRDVPGYEEGMTVLPGRIVSTERKIV
                     QVPTVAVDSIENLDRVHLDLRPLFDNDGLATADERKSSNFDNPQGIFGASYPAQELEK
                     SFIDGRLEISDVEFAITTKGRDNMRLTGQAVTFVPEVFSRICILGAANHGDYTGQAML
                     VYEDGTTQNIAVSFSDWCGGPSKGEDIAFTFPSRYDNTGNIERIKCMLYYRCAETLDK
                     PLKTIVFPQIPNVHIFAITLRRK"
     gene            complement(17899..19224)
                     /locus_tag="PLD15_00740"
     CDS             complement(17899..19224)
                     /locus_tag="PLD15_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62451.1"
                     /translation="MKKLFVLFFIVVSVFLAAGTVRIPVSVNSFTGEPIPVSLGLSGL
                     LDMIGVDFDANWDSLRVIQDGKEIPYQIDDTDLTGRLSSGDTFSFLITGPAEIVVSDD
                     FDIQAPTYAAAGNVVEEDGNWLVEVGDIRAMVNAKGLVRVTGFGAIEGTIVDEIGVAR
                     VAGWVGSTFYVDGQYGRHEEKTSGDFPVVEVAVLPAGPVGVTVVGTLDGKPFLGLTQK
                     IITTIFTNGDIVSRNIFEFGTYADLMKLQVMATRPLTDIAEDTVHTLPVFRRLLWADQ
                     LNITPLEYWLERNAIMYTGNKPYIVFPAVDSMRPLWWGATYIFASQESWRANYSPSLK
                     TGIAEINMWVPPITANYQEWMGGPTWVYESREFRDGLFEWMPGEIDAFESTKGAISSK
                     WEDYVAHFVAGDVYVFSKVYSVYNADSIADSIEFVELKKAEIHSLVFGE"
     gene            19582..20562
                     /locus_tag="PLD15_00745"
     CDS             19582..20562
                     /locus_tag="PLD15_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB62452.1"
                     /translation="MAAKLSDVAKLAGVSTATVSRVLNESGYVSKKVREKVMSAVDEL
                     EYRPSRVASYLASKKLDFDIGVLAGKKVSKILRDSSDQFYTIVFKGIQEFCETNNMNH
                     RLFPVEEFPGDLDGYLLVGGEINEENVKKSKATGRPVVLVDQYLAGVKVDCIVSDGYD
                     GAVYGIKKLLSGGLKRIVNIHGPLSHFGFKDRYDGYVVAMESAGYLPRSFEYDEDNDN
                     MGAIIDLMLSRYGLPDAVFGCNDTAAIRAMEELKARNIKIPEDVSVMGFDDIVNATVA
                     SPALTTFKIFKREMGVAAARRLLNLLMNSEPHPMKISLFTEFVRRESTTV"
     gene            20577..21827
                     /locus_tag="PLD15_00750"
     CDS             20577..21827
                     /locus_tag="PLD15_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB62453.1"
                     /translation="MKKSIFLMLIVCLSIAAIGVELVFWTAPNPLQERFWKETVAEWN
                     ASNPDIQIKWSTIPAAGSSEEAILTAIASGRIPDISTNIFSGFAAQLIEADILVPLDS
                     FPDFWELVDTRKMRSIVEKWKFGKNYYVFPIYSNPIMMWWRMDILRELGYDKPPRTYS
                     EIYEISSKYSIPNERFGSLVVMGRNWYDRWFDFITYYYAASGGEPYLDVEKARALFNN
                     EAGKEVVGFIDKMFRSNWTAVDLGSNPFYTGVVLGGIFGPWEINQARSLFPDVFDDIV
                     ITPPPVPDTYPTDEPIYTFADTKGLVMFATTKHKEAAWKFIKWVYSDIEKDRRWMETT
                     NLPPAREDLLTNPIFKEYMDGNPYFAAYASHVAYAVPPALTTKTVEVQDIMTTFLIET
                     IMYGKSDPLSALSDSSSRVRKELF"
     gene            21853..>22333
                     /locus_tag="PLD15_00755"
     CDS             21853..>22333
                     /locus_tag="PLD15_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62454.1"
                     /translation="MKKRLKTVEAIKGWSLSGMYLVYTAIFWGYPFVWLIILALSKWN
                     FLTPRKFIWFDNFIKLFQDELFWRVFINTFNFMLYFIPIVIGFSLLFALGLKRVRLFR
                     TFFILAFLVANVSSGVSYSIIFQKLFAVNGPLNDLTRALFGVTIPWFSSPQLATLSIA
                     "
BASE COUNT         5696 a         5521 c         5908 g         5208 t
ORIGIN      
        1 aggccatgac cggaatttac cacaaccgaa acacagtcac attaacggag ctcaacaaca
       61 cactggatcg gaccagcacg gataagctga tatattggtg gtcgaagaac ttcgagaccg
      121 aaggtggtta ataaattaga gaggcggtat tccgattgac ccgtaccctt ttctaccagg
      181 cctcctgcgt attttctctc gaaagtctat cattcaaaac aataagtccc ttgaatattg
      241 tttctgtata gctttaatgg agagtcatat ctgagcttct attgttcaat ttagattgtt
      301 cgctacttta ttgacagtga tgatatcgag aattctaact gtcgacggac cgtttacagt
      361 gcttgcccct acggacaaag ccttggtaaa gctagccgaa ggaaccatag aatagctgct
      421 gaagtacatt accgatctca agagtattct ccttggacct actcgaaaac tctaggaaaa
      481 gagctttctc ataatttaga atcttattag ttgagagcca gagatacatt aatcgggggt
      541 gcctatgaaa ggcaatttct ttccagatag tagatacgac gttgcaataa tcggttcggg
      601 actcggaagc cttgcggcgg cttctttact ggcaaacagg ggtctcaagg tactggttgc
      661 cgaacggcac tatcttccag gcgggtgctg ctcgatcttc agaagaaacg acttcacttt
      721 cgattcggcc gtaggaatgg ttttcggatt cggcaaccgc ggcttcaact ctcacagatt
      781 cgttttcaac gagatcggag aggacatcga cgtgataagg catgaggccc tttactccgt
      841 gacctttgga gagaagagaa tcatattctg gccggacctc gacagattca ttagcgagct
      901 cgggaggcat ttccccggct atgaagacca gctcagaaag ttttacgact atctcgccga
      961 cttctatcac aacgtgatcg tggccgatcc cgtgatagtg cctccgactg aaatgaagag
     1021 gagcgacagt ctcaaagccc tgctcaagca cccggtgcgg caggccagga tgatcagatt
     1081 actcttcagg agcaccgaag aactcatgag taagttcatc gacccgaaag ctgaagagct
     1141 ttatcagttc ttcgatgttc tcacttccac ctactgttat acaacggtta aagagacacc
     1201 cggcgttctc gcggtgacta tgttcataga caatcacgag ggcggtggtt tctatccctc
     1261 tggttcctcc cagatgatcc cgaacaaact tgagaagtca attgaaagaa acggcggtta
     1321 tatgctctat gagaacaggg ttgacgagat cctctttgaa gacggcaaag cggccggtat
     1381 taagctcgag gatggaagcg agatcgggtc gaactatgtt atctacggcg ggacggtatg
     1441 gaatctttac ggagggataa tcccaaaaga gcgcatcggt cctgagaaac tgaaatgggt
     1501 cgaatccctc gagccgacct tccctgcgat cgtcgtttac gccgccgtcg attattcggc
     1561 cgttccgatc ggaactaatc ccgtcgaaat gttcgtcgag gagttcgacg agatagccga
     1621 gagcgatgtc acagtttata tatcctcgat cgacgacccc tcgatctgcc cggaagggac
     1681 tcatgtaatg tccatgatcg ctccgtcaaa gaagaagtgg cctccgcggg gcaccgaaca
     1741 gtacaaagag atgaaaaaag cagagaccga gagagttctg aagctggtcg agaagaaatt
     1801 ccccaaactc agggattctt tgaaacatct ggaaacggcc acgccgctca caatcgagag
     1861 gtacacgcta aaaaacgaag gccgggtcgg aggtccaaaa cagtccatcg gccaggaatt
     1921 gctgaagaga cttcacgcca ggagcgaatg gaaaaacctg ttcatatgcg gggattcgac
     1981 cgtgatggga atgggaacgc cggcggtcac agtctccggt atcggtgccg caaacttgat
     2041 tctcagagat ttaagaatga aggagtacag ttacagagag cccgaagtcg agagggttca
     2101 ctatctttcc gaaaaaaaga ggaaggcggt ggatcttgaa aagaccgcgc tcgatcgcga
     2161 tacggtccct ttgattagcg gctcctgtca gctgtgcgaa atccccggct gcaagatgaa
     2221 gtgtcccgac ggaaaggata ttcggggtgt tttgaggcgt ctggagtcgg gaaacttccc
     2281 gggggcgaag cgacggcttc tagagacgaa ggtgagcacc gattgcgccg actgcccggg
     2341 atactgcgag aagagctgca gaagactctc cttcgacgac aggcccgtac ccattaaaag
     2401 gcttcttagc tggctggaaa aggaagtgat aatatgaaat tcgattgcat cgtgatcggc
     2461 tcgggaatag ctggtcttag ctgtgcggct ttcatagcga aggctggcaa acaagtcata
     2521 ttgatagaga gagcggaaaa acctggtggc tgtttctcat ccttcgagta caacggttac
     2581 catttcgaca gcggaataaa ggcagtcgaa aacgcggggc ttttatttcc aatgcttgag
     2641 aacttcggta tcatgggtaa gctaaatttc gtaaagaaca gagtgtacgc tggcttcgag
     2701 gacaaagtgt tttccatgga gtcggacgaa agtcttctgg agtacttcga cttcctcggt
     2761 gaacgctttc ccgaaagcag atccggcata gaggatctac ttaaggaaat gcaagaactg
     2821 aacagcttta tgggttcgtt atcaggaatg tcgggaccgc tttacggaaa gactacgttt
     2881 ctgaccaagt tgaaatcaat tccctggttt ctcaagaacg gtaagaaact tctgggcttc
     2941 ctgaagaaac tgaaatacaa caatataccg ttcagggatt tcctcaaagt tagaatcccc
     3001 gacgacgagc ttgtcagtat catagacgag cccttcttcg aggggactcc cgcgttcttt
     3061 gggctggcct acccgacggt tttcagggat tacttctatc ccgaaggcgg gatagagaga
     3121 ataccgctca ccctcgctga ctttatcagg gagaatgggg ggaccgtcct taccggtacg
     3181 gaagtgaccg ggatacttct cgaacgaggc ttaccttcgg gggtagtaac cgataccggt
     3241 caggagatcg agtctccgat tatcgtgaat gcagccgatg cgaagcggct atacaacaag
     3301 ttgctcccgg agaattccgt agatcaagat ttccttcaga ggctcaacag agcgcaggtt
     3361 tccgactctg tcttctccgt atttatggga gttgacacag ctccagagaa tcttgatcaa
     3421 catgtggaac acataatcta tgttccggct gcagccaaac cctgtgacta tgaatcgccg
     3481 gagtacttca aaaccacggg aatggagatc agcattccct gcatgagaga tccttctctc
     3541 gctccagccg gcaagacggg ggtaattctg aatgtcaata cgactcagaa ggccctgaac
     3601 ggctggggaa gaagtgtttc cagagcggaa tatgaacgcc ttaaggaaca ggccgccgac
     3661 gatgtaatgg ccaacttctt caggctttat ccttcactta gagagaaaat tgagttcagg
     3721 attgttgcca ctcctttcac catggaagag aaaaccctca acagcggcgg ttcgattacg
     3781 ggatggaact accatccatc cgagactttt cccttgaggg acttcagaaa gatgaagaga
     3841 tcgatcgata cgcccgtaaa gaatctcttc cagataggcc agtggagctt cgatccggca
     3901 ggagtgccgg tttgcctgat tacagccaaa ctggctgcag atagagttct aaatatctcc
     3961 cggccgtaaa gactgtaaag tcagcttctt taagatgaca ccgtagggct ctttgagcca
     4021 gatcggatag atagcggagg tatcgaggtt gaaaaaagca cttataaaag cgctcaaaag
     4081 cgcaaaggtc gagtttgtaa ggtctctctg gtgcgataac gccaacgtga taagggccaa
     4141 gtcgatagat atcgatttgc tcgaggacaa tttcgagacg gtggccggta tctctcgcgc
     4201 gcaacagggg gtgcctgtca tgtacgacgg cgtggtgcct gaaagcgggc tgatgcctgt
     4261 tggggagata tggctcaacc cggactggga tactctaaac gtactcccct acaccccggg
     4321 gcacgctagc gttatttcgg atctcttcgc cggccgatac gaatggagtt tatgcccgag
     4381 gttttttctg aaaagggcaa taaaagctct tgacgagctc ggtttcacca tgagggccgc
     4441 cttcgagaat gagttctatc tcttcgacag gtctctgaac ccggtggaca acacgctatt
     4501 tgccagctcc ttttcgatgg acctcaactc gaaggttgtg ggcgatatag tcagaaacct
     4561 caaagcccag ttcctgaacg tcgagaggta ctacgccgaa tcggggccgg gccagcagga
     4621 aatcgtcata gctcacgccc ctgcgctcga ggccgccgac aaccagatca ctttcaggga
     4681 aaccgtgcgg accaccgcgg tgagaaacgg tctgatggcc tccttcatgc ccaaaccttt
     4741 cgaagagttc ggagggagcg gctgccacct gcatttcagt cttttgaaag acgggaaaaa
     4801 cgtaatgaga gaccacaacg gtgagctttc ggaggttgcg ctctatttca tggccgggct
     4861 tctggaacat ctcccctctc tcatggctgt gacgacaccg accaccaact cttacaggag
     4921 aataaagccc tcctgctggg ccggtgctta cagatgctgg gggcttgaca acagagaagc
     4981 cgctttgagg gttccgacag atccctccgg cagcgttgcc ggccacgtcg aattgaagag
     5041 cgtggatgct tccagcaatc cgtacctcgc cctgggaatg atgatcttcg ccgggattga
     5101 cggattgaag aaaaaggtcc tgccgccggt caacctgcag ggagatccgg ccttgatgaa
     5161 cggagaggaa ctgctgcagg gcatggtaga acctcttccg tcctccccgg gaatcgcgct
     5221 ggatatgctc gaaaacgacc gataccttct gaaagtcctg ggcgaggatc tgtcccgggc
     5281 ctatttggcg gttaagagag ccgaatggaa ggctatggga gagctgacga tcgctcaaga
     5341 gcgccagatt ctactggagc ggttctgatg ggactggatc tatcgtcgct tcccgttttc
     5401 gacggtcatg cacacaacct tctcctcccc gcatggctca aggagtatcc tatcgaaggg
     5461 atattcactc agagcacgga cagggagtat cttaactctt ttgcaggcga gacgctcgcc
     5521 ttcaagagaa gtctcaagga tatcgccgcc gttctggaat gcgatccctc gatcgaagcg
     5581 atcaggaaca ggcgtgaaaa gatggaacag accgaactgg ccggtctgta tttcgaaagg
     5641 gcgaatatct caggcgtcgt tctggacgat ggcttctggt tccaccgaac tcgccctctg
     5701 gagtggcact caagtttcgc gagcacatac agggctttga gaatcgaaag ccttgccgaa
     5761 gctttactcg aagactcaac ctctttcgat tcctttcttt ccgatttcag agatgcactc
     5821 ctcaatccac ccggaggcgt cgtggcttac aaaagcataa tcgcttacag atctggtctg
     5881 agaatcggag tgcacgacaa aagagcggcc agagacggct atctagttga aaaagagagg
     5941 aacaccggaa ggttgaccga tggaaggttg ctgggctttc tcttcaacct gactctcgaa
     6001 gtctgcaggg gcaggggtct ccctttgcag atacacaccg gttggggcga ttccgacctc
     6061 gatctgcgcg aatcgaaccc gctgcatctt aaacctctac tagacgagga taaatactcc
     6121 cggaccaggc tggttctgct tcattgttac ccgtacgcac gggaggcggc gtatcttgcc
     6181 gcgatttatc cggaggtcta tgtcgatttc ggcctctccg ttccccttct aagtgttgcg
     6241 ggcatggagg cggtgatcgg agagatgctg gagatcgcgc cggtcacgaa gatctgttat
     6301 tcctccgatg cgaggttcat gcccgaaggt ttctatctag ccgcaacctg ggccagaagg
     6361 gctttagggg agtctctgga gcgatcagtg cggtgtggcg aattgatgtt gaacgaggcg
     6421 ctgaaaacgg ccgaaaggat attgaaagaa aactcggagg ggctttacgg agtggcccga
     6481 tcggctggag gtgaagcata agaatgacga tgcatacagg gggtaaggag atgcttcttc
     6541 agagagaaca gagagttgtt tttcagggcg atagcgtaac cgataccggg agagataggg
     6601 acgacttctt cggtcttgga aacggttatc ccgcgatcgt ctccgggacg ataggttcgc
     6661 ttttcccgga gatgcggatc gagtttctga acaggggcgt gggcggcgat agagttaaag
     6721 accttaaaaa ccgctggaaa gaggattgcc tggatctttc accggactgg gtctctatcc
     6781 tggtgggaat aaacgactgc tggcgtcttt acgacagcaa cgacaggacc gaaccggccc
     6841 tcttcgagag caactacaga taccttctcc aatcggcggc cgcgaggggc gcatcgctga
     6901 tcgttatgga acccttttat ttgccagttt acagagagat ggaaatgtgg acggaagatc
     6961 tgggaccgaa gatacagata atacgcaggc tggccagaga gttcaaagcc gtgtacgttc
     7021 cactggacgg aatcttcgcg gaggcagcca caaagagaga gccttctttc tgggccgaag
     7081 atggaatcca cccgaccccg gagggccatt cattgatcgc gagacactgg ttaaaagcct
     7141 tgggagttct ataaaaccac gatcggttcg tgtcgggccg tcttctagat ggccgtcgcc
     7201 ctgtttttac cccttctctt ggattcatac agggctctgt cgaccctgcg caaaagtccg
     7261 tcgtagtctt tctcgccgac ctcaagtgat gagacgccca tgctgatact cagtttgtcg
     7321 atcgcttcga actctccgga atcgatttcc gttctcagtt tctcggccat ctcgagcgct
     7381 ccatctagat cggttgaggg agccacgatc aagaactcgt cgcctcccca gcggcctaga
     7441 gagtcgcttt tacggatttt agagagaatt tgaaagctga gcgccttgag agccgtatcg
     7501 ccggcgtggt ggccgtgggt gtcgttgatc ttcttgagat catctaagtc gaacattatc
     7561 accgaaagcg gctcgcggta tctcctggat ctttcggttt cgcgttccag gagctgatcc
     7621 agtgagtacc tgttgagcac gcccgtgagc atatcggttg tggccagtgt cctgtacatc
     7681 tcctggcttt ccttgagcgc ttcttcaatc cttttacggt cggttatatc cgtcagaagc
     7741 actatatgtc ccctcagtct tccacgtttg ctcattattg aagtctttct ggattcaacg
     7801 atcctctctc cgataaccat ctcggaccgc gagtccgtcg atccgatgaa acccagcaga
     7861 gccggcatac gggaaaaggc atcgtctatg gggagtccga gagccttctc tccgtcgatc
     7921 tcgagaagct ttaccgagac ggggttcacg tctatcagga tgtccgattt gtcgataaca
     7981 actatcccgt cctccagatt tctgatcacg gtctctttgg cgatgggacg gagatcgaac
     8041 agcctgaacc tgaaaatgct caaggccgcg aacagagacg tcaggaaaaa agcgaccgga
     8101 gtcaggtcga tataccccac cggaaggatt ttgaagaggt agtttaagtt gactagtatc
     8161 gggaaggcca tgcccagaag tataatactc agctgcgttc ggtaaggtct ctccgaatag
     8221 aaccactccc tgaggagcaa tactatactg cccaccagaa gaacgtaagt ataaacgata
     8281 ttgatccaga aaagcggacc gtgaacgagt ttctgaacgg ggaagggcca ctgtgtatct
     8341 atctcgctga ccgtgtagaa aagccagtgg aactcgttgg tccacatggt cagattgacg
     8401 atcgcgggta caatgaaaag caggaccact cttttgaacg tgaaccattc tctcctgtcc
     8461 agaatcgcca ttacaaacat gaagacgaaa accggcagcg aaacgatcgc cggataggag
     8521 atactgttca gcagacgcat ctgctgcaaa tcattgctca gaagttccag accgtacata
     8581 gtggaccacc aggtgatggc taacatcagg aagccaagag gccccgctcc ggtcaactcc
     8641 cgtttataga agctgtacat ccccagcaaa ccgcaagaaa ctgtgggaat gaacatcaac
     8701 aaggcaaatg gattcatcta aacagatagc cttcccttca gagagatcgt cagaagtatt
     8761 atacatttcc aaaatgaatt cctggaaccg gcgtttgtct ctttcagaaa atcgtggtca
     8821 ccaggcgaag aactcctccc ggaaaagtcc ggtctaacac gagaggtccg gagaattgta
     8881 caactttccg acccgcaacc acaacaaata ctggaggttt gaagttatga gaagatcggc
     8941 aataattgtt ttttcagtct tgactgtgat ttctacaatg ttattttccg tcatatcgga
     9001 agatatcgtt tctattccgg cgattataga tgtggtcagt attgaacagc gtctgaaggt
     9061 tgagctggta gaaaacgctt ccacgggata cacctggtcg tatgaagtct ccgatccttc
     9121 ctttctgacg ctggtttcga aggagactgc ggaaagctca gtagacaccc ccgtaatagc
     9181 cgggagtccc tccaccgtca cctggctctt ttctcccaac aagagcggct ccttttctct
     9241 ggttttcagg ttgttcagag catgggaagg cgaagagacg gcgatcgacg tgagagtgta
     9301 caacgtcgaa gttcagaaca gcagcctgag tgagtgcgtt ccacagtatg tgaccattct
     9361 cggcagttcg tccggagaga tcgaaatatc gcagttggcc aggatagtcc ttcaggagaa
     9421 cccttcaacc ggttactcct ggaaggcaga agtcttcgat gcagccgtgc ttagaacggt
     9481 cgaacgctcc ttgctggccg aaagcgattc acacgaagct acccccattg ttgagctggg
     9541 aggtagtcag gaagttctcg gcgcgccggt taacgtagcc ttcgacttcg aagcgctgaa
     9601 ggcgggcatc tctcttgtga ggttcactta ctcaaggccc tgggaggaaa gcgagcagga
     9661 caaaacgctg ctcgttggaa tcaaggtggc cagataaacc ggaaggatca agtatcgtca
     9721 acgatttcgc gtcttcgact taatcgaaca tcgaactgag aacgatcccg cggagatttc
     9781 ccggtagtat catagtcgta tatcccctgc gccgtccgac aagtcgcggc gcaggatata
     9841 tactattaat aacagttcga ttatctagaa aggagatcga tcaccgtggg agttgaaggt
     9901 ctcgaagtat ggttcataac aggaagccag gagctttatg gaaaagaagt actgaataag
     9961 gtggagagca actcgaagaa actggtcgag tatctgaacg accaggccgg gttgccggta
    10021 aagatcgtat ggaaacccgt cttgaaagag tcgcggcaga tcagaaccct tatgaacgaa
    10081 gccaatgtcg acgacagatg tataggactg atggcctgga tgcacacttt ttcgcctgga
    10141 aagatgtgga tcgccggtct taaagccttg agaaaaccct ttcttcactt tcacacccag
    10201 ttcgaccaga agatcccgtg gaagagcatc gacatggatt acatgaacac caaccagtcg
    10261 gcccacggcg ggagggagtt cggctttact gccaccaggc tagatataag gcggaaggta
    10321 atagcgggcc actggaagaa tcccgagatg atcgcaagag ttgaaaactg gatcagggcg
    10381 gcgctcggct ggaatgaaat gcaaaacctc aaggtggcga ggctgggcga caacatgaga
    10441 gacgtcgccg tcaccgaagg caacaaagtc gatgcccaga tagatctcgg gtacgaagtt
    10501 cacggctacg ctatgggcga tttcataaag ctggtaaaga aagtgaaaga aagcgaaata
    10561 gaagctctcg taaaacagta cgggcaggaa tatcctctca ccagatcctc tgtctcccag
    10621 gaggagttca aaagttcttt gcacgaggcg gcaaagatcg aaatagcact cagacgtttt
    10681 ctcgatatgg gtccgttcaa agccttcaca actacttttc aggatctgta cggccttcgc
    10741 cagctccccg gtctggccgt tcaaaggctt atgagagacg gctacgggtt cgggggggaa
    10801 ggagactgga aaacggccgc gctggtaaga acgatgaaag tcatggccgc tggcagagac
    10861 aggggaacat cgtttgtgga agattacata taccattttg aaccgggcaa catgaaggaa
    10921 ctgggttctc acatgctcga agtgtgtgaa tcgctcgctg acggcccgat ttccctcgaa
    10981 gttcatcccc tttctatagg agacaaagag gctccggcga gatcggtttt cgattccaga
    11041 gaaggggagg ctgtccaggt atgtatcgta gagttcggag atagattcag gatgatagtc
    11101 aacgaagtgg aggtcgtcaa aaggcggcag gaggaagaga tgcccggact tccggtagcc
    11161 agagtgctgt ggattcccaa acccgatctc ccgaccgcgg caacggcctg gatactggca
    11221 ggaggagcgc accattcctg cctgagttac actgtcaata ccgagcaact ggaagacctt
    11281 tgcgaaatgc tcgatataga gttcgtggtt atagatagag acacgaagat ccgtgacttc
    11341 aaaagagagc tgagaaacgg cgagatctac tacaaattgc gaaaactgtg atatcgcact
    11401 gaacgcaatg tattcataag taccgtagaa gctttgcgaa aggaagtcgg agtatgctgg
    11461 aagcactaaa ggaaaaggtc ttcagggcca atatgaagct ggtcgaggcc ggtctcgtga
    11521 ttctaacatg gggcaacgcg agcggtattg accgcagcaa ggggttaatg gtgataaagc
    11581 ccagcggggt ggattacgac gaactctcgc ccgaaaagat ggtggtggtc gatttgaacg
    11641 gcaacgtcct ggagggaggg ctgaggcctt cctccgatac gccgacccat atagtcctgt
    11701 acaggaactt cgagggcatc ggcggcatag tacacacaca ttcaacatgg gccacggcct
    11761 gggctcaatc gaggtcgccc attccttctc tggggacgac tcacgccgat cacttccaca
    11821 acgaaatacc ctgcaccaga ttgctgagag aggaagaaac ggaagaaaac tacgagatga
    11881 acactggcag ggtcatcgtt gaaaccttcg ccagtaagga ttactttagt actcccggag
    11941 tcctcgtggc cgggcatgct cccttcacct ggggcaaaaa cctggatgat gccgtaaaca
    12001 acagcgtggt tctcgaagag gtggcgagga tggcctttct gacgaagatg ctgaaaagcg
    12061 acgttgaaac cctgcctgcg cacataatcg ataagcatta ttcgagaaag cacggaaaga
    12121 acgcctacta cggtcagaaa aaagcgccgg gtgacgcgcc tgaccggtaa caggtgaatc
    12181 tggagctggt tttcgcaaga gattacatct gaaacagtta aacctcaggt gcgtgcgcgc
    12241 cttctcttcc tgtaagcaaa gtgcagaatt aagaagtaaa cgacgcaaac aacggtgaag
    12301 gcgaatatca cgatcattat cagttgtacc ttcgtgctgc tcaagaacag ctctgtgttg
    12361 acggcaatct gataaactgc gtagagactg gctaccagca tacaagcggc gagtatgccg
    12421 atctccattc ttcttttctt gatgcgctct ctcgtttcag gtattatcct catgcggaac
    12481 accccggcga attcggcgtt aaccttcgat acagtagagc tattatacca aaatgtacgg
    12541 gtttttcacc cctttatttc atcgctctcc acctcgttct tagccgtcct ccgtcgccgg
    12601 gagccgggac aagcgaatag ctattcttaa cttccagtat taggcctttt gattcaattt
    12661 atgtatataa aacgacagaa gcggcccgaa ggccgcttct acagcaagat ttttaaccct
    12721 ttttccccag ctcggatgtg cagttcggac atctcgtcgc ttcgattgga atcgaagtgt
    12781 gacagaatgg gcatgtcttc gtcgttggag cggccggagc ctcttctttc ttctggcgtt
    12841 ccctcatctt gtttatctgt ctgatgatca ggaaaatcac gaatgccaga atcaggaagt
    12901 tgatcacggc gtttatgaag aggccgatgt ttatagttgc agcaccggcc gcctttgctg
    12961 ccgcgagtgt ttcgtaactc ccgccggaga ggttgatata aaggttactg aaatcgatgc
    13021 cacccgtgaa gagaccgaga atcggcatta tgatatcatc cacgagggat ctaactatta
    13081 cctgaaaggc gcccccgatt atgataccga ctgccaggtc gatgacattg ccgcgactta
    13141 tgaacttttt aaactccttc cacatggctc tcaccccctc tattttggtg acactgcgat
    13201 tatattccca aaagacttta aaacaaagaa aaagagagac ctgaaaagcg ctcttagaga
    13261 tgtgtgagaa tcgctgtatc atgtttttct cgttcttgtc agaccctaga ccccacatcc
    13321 cggatcccgc tcgcccctct gatctctctc cttccgacga atcacgtaga aatccaacag
    13381 aactattgca atacaaagtc aggcatgata gaatactaac gaacgttaaa tataaaggtg
    13441 tgtacaatgg cggagaagga tttaaaagag agaatcaagg aagtggcggt tgagcatttc
    13501 aacagaaacg gttatcacgg aacgacgata cgcaacatcg ccaacgatgt tggatgctct
    13561 ctaccgatga tctattatta ctacaagaac aaaaaagagt tgttcgacga aataatcaaa
    13621 aaggagtttt tcagcattat aaagaagcag gcttcgctcc tgaaaacctc gagtatcata
    13681 gatttctaca ccaggttcat ccacgacctg aacgccctga atggctacga taaacaggtt
    13741 taccgcctcg ggataaaagt ctatctctcc ttcgacggtg acgatgaatt gatgaatcta
    13801 atggatgaat gggaaaagac aatactgccc aggcattacc ggattctgca gcctcatctg
    13861 aagaacaccg ataacgacac ggcggtcgtg cggacactgg ttcatttgct ggaaacgctg
    13921 atcgagaaca tagtcgtaaa gaacagatac ttgcccgaag aggagatccg ggaagaaatc
    13981 gccatcgttc tgcgcggtgt ggacggttca attcccgatc cgcaattaca ctgaatataa
    14041 gctggcatat aaagatgcgg ttttatcatc tacactttat taacgaacgt taaaacaaca
    14101 aagaggagag gagaaaagtg gaaatcgaaa agagactcgc aatactgcag aacacatacg
    14161 cggcatccgt ggccgaagcc gtcaacacat acgacaggtt aaaagagctg gaggctgtag
    14221 ttgagagaag aaaagaaagg caggcccaga ctgctccata catgaatcag cagctgggta
    14281 tcgagagtgt ggaagatgtc ttctacaagc tttcggaaat ctacggctgt gcgaactgga
    14341 aagtggagaa aacaggtgac gactacgtcg ccacagcgac ttcatgtaag ctctgcgcgt
    14401 tgtccaagaa gatgggcggg gcaaacccct gcagtgggtg gtgtctcgac ccaatgatcg
    14461 ccatgctgtc ggcggccgtt aaaatcgact ccagacacat aactgttgaa agtactttaa
    14521 tgaaaagcga taaatgtaaa atgttaatta atacaaaaat tgaatagcaa ttgaagctag
    14581 cgaatatata aattgactta cgtagaagtg tcatataaaa aaccggtgtg attacgcaca
    14641 aaaaattatt tgctttttac aggaaggccg acgtagaata taaacgttta ttttggaaag
    14701 caatcgaggc gatgatcact tggaaaacct aaaactcaac aggacgctat ttgctatcgg
    14761 gatgttcgta gtggtattcg ttacgcaagt gttcttagga agaatcggac actatatcgc
    14821 cagtgtgatt tcgtacggca aaatcgatcc ttacgatgct tttgcgggca tttccattca
    14881 ccacatcgtc cagatggttc ttgccttgat tataatcacc gtgctcggca agcttctaaa
    14941 gatcgatttc tatctgaagc caggcgacgt agccagagga aagaaatacc tgataacttt
    15001 tacctccatc tttgccctta ttgcagtcgc gctccacgtt ttcatgtaca tcaacaatca
    15061 gcttcccgta tataccttcc ctctggacaa aaggaacgtt ctggggactc tgggtttcca
    15121 gttattgata agcgggacgt ctgaagagat agtgtttcga gcgctgccgg taactatgct
    15181 ggtctatgcg ttcggcaaga gcatcccggt caagggtcat gttacgctgg aagtcattct
    15241 tgcatcgatc ctgttctcgt tggcccacac aaaatggtcg ctgatgccct tcaaatttga
    15301 gatggatacc tttcagatag tgtattcctt tatacttgga acgatacagg gcgttgtata
    15361 tcagaagagc aagagcattg tgtatccgat gctgatgcat aatataagca atttcctgat
    15421 ggtaggcatg gggtatctct tcacggcctt gaaggccatc acaagttgac cttgtcacgc
    15481 tgcgaaattt aaagagcgga ccttacggtc cgctctttta tatgcaaggg gggatcactt
    15541 ccttctgagt gttatcgcga atatgtggac gttcggaatc tggggaaaga ctatggtttt
    15601 gagtggtttg tcgagagttt cggcgcaccg gtagtaaagc atgcatttta ttctttctat
    15661 gtttccggtg ttgtcgtacc tggagggaaa agtgaaggca atatcctcgc ccttcgaggg
    15721 cccgccgcac cagtcggaga aggagacagc gatgttctgt gtagttccgt cttcgtaaac
    15781 cagcatcgcc tgtcccgtgt aatcgccgtg gttggccgcc cccagtatgc agatccggct
    15841 gaaaacctcc ggaacgaagg tcaccgcctg gccggtcagg cgcatgttgt cccttccctt
    15901 cgtcgtaatt gcaaattcta cgtcggagat ctccagcctg ccgtcgatga agcttttttc
    15961 aagttcctgc gcgggataag aggccccgaa gatgccctgc gggttgtcga agttcgagct
    16021 tttcctctca tcggcagtgg ccaggccgtc gttgtcgaag agcggcctca gatccaggtg
    16081 cacccgatcg aggttctcga tcgaatctac ggccacggtc ggcacctgga ctatctttct
    16141 ctcggtagat acgatccttc ccggcagcac ggtcattccc tcttcgtatc cggggacgtc
    16201 tctgtagttc aagaccagcg tttcatcgcc tggtatgtac agggcgatca ggtcgggtaa
    16261 aagcaccagc tgatccagat cgaagcttcc cgattcgacc tttcccccca gcgtgagcgt
    16321 cgattcctga gcggtgccgg tgataacacc gactttgaag atagatttct gggcgatgga
    16381 aacggtctct tccgatctcg actcccccag acggagggtg aaagagaccg gaccggtctg
    16441 aacgttaccg atcccggtga aggccgaata aggcacagga ggaagcgcaa cgctctctct
    16501 cagactgaag acaccgctta cttttagagc ggcgtttccc gagatccccg taccctgaaa
    16561 tgtatcgact tcggaaaggc cggtcctcat cgcttcggcc tctatcacct ttgcgggcgt
    16621 aagcaactcg atcgcgcctg agaagtactt ttcatattct tccggcaacc tcgacactct
    16681 ttccagcgaa agcagcatgg atatcgtcga ttcggctccg gcgtttctgt ttatgtggga
    16741 gaattccata ccgtcgtacc cctctccgtt cggtccgatc atggactggc ccagtggatt
    16801 gagaccgatg aaccagctcc ccagaagcgc ggtgatcgtc gcgatctccc ggtcattggt
    16861 aatggagaag agcgtgtagc cggtcgaaat ggccgcctcg gcggcgtagg cgatctgggg
    16921 gaagagctgg acgaatccgg ttagagagta caccggaccg gcgccgagca gaagcgtggc
    16981 gccttctttg aaggcatcta ccgtgagctc gagcagttca tcgtcttcca gcgccagcgc
    17041 ggtcatcgcg agcgcttcca gctgtctgga gccccagccg tgccagtgag gaacggtctc
    17101 ttgcggagcg ttctcgtcca ccagacctct gaagatcgag tcgtcatcgg tgatcagttt
    17161 cgatataagc gagccggaac agcgttttat gaagttcagc gcttcgaggt cgttggagat
    17221 tctgtaaagc tcgacggcgc caaggagcgc taccgatgag acatctgtgt atccctggat
    17281 cagaccgtct ctttcgtagt tgagcaaaac tctgaaggcc ttctgggaag ccctggccag
    17341 ctctatggag taggaagggt ccctctgacc gagtatccac gcgcccgtag ataaagccca
    17401 gaacccgcgc gccgtccacc agttgccgcc tttgcgcgag gtgggaccgt tacggtttat
    17461 cttcccgtcg tcgaagatga aattgaagaa gtcgccatcc gcatcctgca tggccagaag
    17521 gaaatcgagg gcttcacggg ccctgaggta gtgggtctca tcggccgagt tctcgaagag
    17581 tctcaggtag agtatggcga ctctggccac gtcgtccacg caggttactc cctctccgga
    17641 cgcttgtgcg tgtctgtaag atccatcggg ctgcctgtcg gcgtagatcc agtagcccat
    17701 ctccacgctt ccctctatgt cgaactcatc tctcagaaat tcgagatggc tcagatcgat
    17761 ctcgacgttt attcccgcga gcgcaacggg cataactatc atgagcaagg ccagaattct
    17821 tctcataaat tattccccct ttcaagagcg agggcgagca aagctcgccc ttacaccgtt
    17881 gtagatggcc ggaggtttct attctccaaa taccagggaa tgtatctccg ccttcttcag
    17941 ttcgacgaac tcgatcgagt cggcaatcga gtcggcgtta tagaccgaat agactttcga
    18001 gaagacgtaa acatcgccgg ccacgaaatg ggccacgtaa tcttcccact tggaggagat
    18061 ggcgcccttc gtgctttcga aggcgtctat ctcaccgggc atccactcga agaggccgtc
    18121 gcggaactcc cggctctcgt acacccaagt cggtccgccc atccattcct ggtagttggc
    18181 ggttattgga ggcacccaca tatttatctc agcaataccc gtcttgagcg atggcgaata
    18241 gttggccctc cagctctcct gagaggcgaa gatgtaggtt gcgccccacc agagaggcct
    18301 catggaatcg acggcgggga agactatgta cggcttgtta cctgtgtaca tgatagcgtt
    18361 tctctccagc cagtactcga gcggggttat gttcagttga tcggcccaga gcagtcttct
    18421 gaagaccggc agtgtgtgca ccgtatcctc ggcgatgtcg gtgagcggtc tggtcgccat
    18481 aacctgcagt ttcatcagat cggcataggt accgaactcg aagatgttgc gggagacaat
    18541 gtcgccgttg gtgaagatcg tggtgatgat cttctgagtc aatccgagga agggcttccc
    18601 gtcgagcgtg ccgacaacgg taacacccac cggtccggcc ggtagcaccg cgacctcgac
    18661 gaccgggaag tccccggagg tcttctcttc gtgtcggccg tactgaccgt cgacatagaa
    18721 ggtcgagcct acccagcccg cgactctcgc tacacctatt tcatcgacga tcgttccttc
    18781 gatggcgccg aagccggtca ctctcacgag tcctttagcg ttcaccatgg cccttatatc
    18841 gcccacttcg accagccagt taccgtcctc ctcgactacg tttcccgccg ctgcgtaagt
    18901 cggtgcctgg atatcgaagt cgtcgctcac gactatctcg gccggtccgg ttataaggaa
    18961 ggagaaagtg tctcccgacg aaagcctccc cgtaagatct gtatcgtcga tctggtaagg
    19021 aatttccttt ccgtcctgta ttactctcag cgaatcccag ttggcatcga aatcgactcc
    19081 gatcatatcg aggagaccgc tgagacctag agagaccggt atcggctcgc ccgtgaaaga
    19141 gttgaccgag acgggaatcc tcacagtccc ggccgccaga aacactgaaa ccactatgaa
    19201 gaaaagaacg aagagctttt tcatgtaaac acctccctgt gatcgtatga aaacgcttac
    19261 acatcacagt gattatagaa ttattttatc tcattgatct aagacgatta aagactacca
    19321 gaggacacag atggacaaaa aatagtgttt attggcattt atttcctttt taaacattgt
    19381 tttatacatt ttttatatat atcagatcaa attaaaatgt aaacttatga taaaaagaga
    19441 tatgaggaga taattcaatt taatagcact taatacgctc tggagtcata ttatatctaa
    19501 ttttcgggca tgaatatcgt cgctttcaaa gggtatagta tttttagaga tgtaagcgtt
    19561 tacataatga ggtgttttct tatggcggcc aagctttcag atgtggcaaa acttgccgga
    19621 gtttccacgg ccactgtctc cagagtcttg aacgaaagcg ggtatgtctc gaaaaaagtc
    19681 agagaaaagg ttatgagcgc ggtggacgaa ctggaataca gaccttccag ggtcgcgagc
    19741 tacctggcca gcaaaaaact ggacttcgat ataggtgttc tggccggaaa aaaggtctct
    19801 aaaattctcc gggacagttc cgaccagttc tacaccatag ttttcaaagg gattcaggag
    19861 ttctgtgaaa ccaacaatat gaaccacagg ctcttcccgg tcgaggagtt tcccggggat
    19921 ctggacgggt atcttctggt ggggggagag ataaacgagg agaacgttaa gaagagcaag
    19981 gccacgggaa ggccggtcgt gctggtcgat caatatctgg ccggtgtgaa agttgactgc
    20041 atagtctccg atggctacga cggagccgtt tacgggataa agaagcttct ctcgggtgga
    20101 ttgaaaagga tagtgaacat ccacggtccg ctctcgcact tcggcttcaa ggaccgttac
    20161 gacggatacg tggtggccat ggaatcggcc ggttatctgc cgagatcctt cgagtacgac
    20221 gaggacaacg acaacatggg agcgatcata gatctgatgc tttcgagata cggactcccc
    20281 gacgccgtct tcggctgtaa cgatacggcc gcgataaggg cgatggagga gttgaaggca
    20341 cgtaatataa agataccgga agatgtatcg gtcatgggtt tcgacgacat agtgaacgcg
    20401 accgtggcat cgccggccct tacgaccttc aaaatattca aacgagaaat gggcgtggcg
    20461 gcagcgagga ggcttctgaa tcttttgatg aacagcgagc cacacccgat gaagatatcg
    20521 ctattcaccg agtttgtaag acgggagagt acaaccgtgt aaaggaggta attacagtga
    20581 agaagagcat tttcttgatg ttgattgtct gcctttcgat cgccgcgatc ggtgtcgagc
    20641 tggtattctg gacagcaccc aaccctcttc aggagagatt ctggaaggaa accgtcgccg
    20701 agtggaacgc cagcaacccc gatatccaga tcaaatggtc gaccataccc gctgccggga
    20761 gctccgagga ggccattttg acggcaatag cctcgggaag aataccggac atttccacca
    20821 atatcttctc gggatttgcg gcccagctga tagaggccga catactcgtg ccgctggact
    20881 ctttccccga tttctgggaa ctggtggata cgagaaagat gagatctatc gttgagaagt
    20941 ggaagttcgg aaagaattac tatgtctttc cgatctactc caaccccata atgatgtggt
    21001 ggagaatgga catactcagg gaactggggt acgacaaacc gcccagaacc tattcggaga
    21061 tatacgaaat cagctcgaag tattccattc cgaacgagag gttcggcagc ctcgtcgtga
    21121 tgggaaggaa ctggtacgac cgctggttcg acttcatcac ttactattac gcggccagcg
    21181 gcggagagcc gtacctcgat gtcgagaagg ccagagcgct cttcaacaac gaggcaggca
    21241 aggaagtagt cggcttcata gataaaatgt tccgcagcaa ctggacggcc gtcgatctcg
    21301 gttccaaccc cttctacact ggagtcgtgc tcggcggcat cttcgggccc tgggagatca
    21361 accaggccag aagcctcttc cccgatgtct tcgacgatat cgtaataaca ccgccgcccg
    21421 ttccagatac ttatccgaca gatgaaccga tctacacctt cgccgatacc aagggtctgg
    21481 tgatgttcgc cactaccaag cacaaagagg ccgcctggaa gttcatcaag tgggtctatt
    21541 cagatataga gaaggaccgc agatggatgg agaccaccaa tctcccgcca gccagagagg
    21601 acctgctgac caacccgatc ttcaaagagt atatggacgg caatccctat ttcgcggcct
    21661 acgcgagcca cgtggcctat gcggtgccgc ccgcattgac caccaagacc gtcgaggttc
    21721 aggacataat gacgacgttc ctgatagaga cgataatgta cggaaagtcc gatccattga
    21781 gcgctctgag cgattcgtcc agcagggtga ggaaggagct gttctaattc tatcattagc
    21841 gggggatggg caatgaagaa gagactgaaa acggtcgagg cgataaaggg ctggtcgctc
    21901 tcggggatgt atctggtcta tacggcgatc ttctggggct accccttcgt ctggctgata
    21961 atactggcgc tgtcgaaatg gaattttctc acgccccgaa agttcatctg gttcgacaat
    22021 ttcataaagc tctttcagga tgagctgttc tggagggtct ttataaacac cttcaatttc
    22081 atgctttact tcatccccat agtgatcggt ttctcgctgc tctttgccct gggcctgaaa
    22141 agagtaaggc tcttcagaac gtttttcata ctggcctttc tcgtggccaa cgtatcttcc
    22201 ggtgtatcat actcgatcat tttccagaag ctcttcgctg tgaacggacc gttgaacgac
    22261 ctgacgaggg ccctcttcgg cgttacgatc ccctggttca gcagcccgca actggcaacg
    22321 ctctcgatcg cca
//
LOCUS       DAOOLY010000007        21822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_2326_length_21822_cov_4.409749, whole genome shotgun sequence.
ACCESSION   DAOOLY010000007 DAOOLY010000000
VERSION     DAOOLY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 21822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21822
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2326_length_21822_cov_4.409749"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="proC"
                     /locus_tag="PLD15_00760"
     CDS             <1..773
                     /gene="proC"
                     /locus_tag="PLD15_00760"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPB62455.1"
                     /translation="AHRLLSKFEGLEALYLLDRYPEKLSGLASDSTRVVLASEPSEIF
                     KNCTHVILSVKPQDCMELFAQIKNGRQHNPIIISTITGIQIDTIVSNTSIKKIARIMP
                     NVPSAIGRGVTGLVFSEGFTKDECREVKRILFTMGDIVEVREKDLAAVTALSGSGPAF
                     VFVIVESLIDVGLKMGLSYEVSRELILGTLGGSVELLKERGNHPGEFRHLVTSPGGTT
                     IEGIYSLEREGLRGTIMKALFDTYLRAKEINAELEGKK"
     gene            770..2014
                     /locus_tag="PLD15_00765"
     CDS             770..2014
                     /locus_tag="PLD15_00765"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPB62456.1"
                     /translation="MTDLAVKGVKVKEASRFLRLASTTIKNQALKSIAGSIDMNRSAI
                     LVANSLDVLQARNREIKESLIDRLALTEKRIDGMIQSCYDVAALSDPVGEIETTWIQK
                     DGLRISRERVPLGAIGMIYESRPNVTVDASILALKSGNSVLLKGGSDAINSNRVIVSA
                     IKRGLEMVGLPEGCVELIEDVSRDCVMEMLKMREYLSLIIPRGGSGLIQFVVNNSTVP
                     VIETGTGNCHIFVDYNADLRKAVSIVDNAKTQRPGTCNAVETILVHEKIAGEFLPMIK
                     EALSRKNVELRGCEKSRQFVEMVPATEEDYETEFLDLILAVKVVRDVKEAVEHIERYS
                     SHHSESILTQDYSNARYFLKAVDSAAVYVNASTRFTDGGEFGFGSEIGISTQKLHARG
                     PFGLRELTSYKYTVMGDYQVRE"
     gene            2014..3078
                     /gene="proB"
                     /locus_tag="PLD15_00770"
     CDS             2014..3078
                     /gene="proB"
                     /locus_tag="PLD15_00770"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPB62457.1"
                     /translation="MSRIVLKVGSNLLVKKNGELDKHYIAELAREIANLKSLENEVVL
                     VSSGAKAAGFGYLSGRSAEHDLYLKQALCAAGQVQLMKLYETVFDLYGEKVAQILVNR
                     DDFGDRKRFLNLRNTLIGLLEMGVIPIVNENDTTSTEEIMFGDNDILAAMFAMGWKAD
                     FLILMSTVDGIYDEEGKVMRIFDSASKIKSMSGSSWGTGGIGTKIRAARGASASGIKC
                     CICNGRELSTIGQFIHGIETGTVFEPIDSSPGARKIWIGFLSTPGGSLIINKGAITAL
                     KRGKSLLAVGVERVEGEFSQGEVVRILSFEGEEIARGISNFSSFEVDRIKGTRSDKLS
                     AILGHDCAKVVVHIDNIYQN"
     gene            3152..3448
                     /locus_tag="PLD15_00775"
     CDS             3152..3448
                     /locus_tag="PLD15_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62458.1"
                     /translation="MYVAVMRYHFHEESLERACSIWKEAVLERATGREGFVRAFIMTG
                     TGGKALAVGMWEEKRHADEFMKTGVFKELLNDFTDMMVANSEHEAYEMKFCSEK"
     gene            3451..4905
                     /gene="pap"
                     /locus_tag="PLD15_00780"
     CDS             3451..4905
                     /gene="pap"
                     /locus_tag="PLD15_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HPB62459.1"
                     /translation="MLDKIDLEARLTSKEYSRTLESLELRLGFLQRKLKERSVPVVIL
                     FEGWEASGKGTAINNLIMPLDPRGFKVFPMAGTGENDRFYPYLWPFWNRLPSGGRITI
                     FDRSWYKVLLDEEKAMSEREFLSHLKTFNSLERLLTDSGAVIIKFFLHIERKEQKKRL
                     EKLDSDSSTSWRVKKRDFRQNKNYETVKMLVEKVLQASDTDIAPWYIIPSTDEKLAAV
                     QIMNTVVNCLEKALERDNEVTGTVNSWSGNTSVIDSLDLSKSIEKDLYEKRLAEAQKK
                     LREYQHLLYKKRKSLVVVYEGMDAAGKGGNIRRLTAKLDPRGYEVIPIGAPNDTEKSY
                     HYLWRFWTNMPKDGHIAIFDRSWYGRVMVERVEGFASEAEWARAYREINDMEEQLHDH
                     GTVVVKFWLHIDLDEQLKRFQARENDPEKRWKITPDDWRNREKWDLYKPAIEEMLVRT
                     STSYAPWTVVESNDKRYARVKVLETLVKLLEENL"
     gene            4992..6305
                     /locus_tag="PLD15_00785"
     CDS             4992..6305
                     /locus_tag="PLD15_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB62460.1"
                     /translation="MKAILISLLCQAILFYGYLSASLALVFSGGGVRSIYQLGVWKAL
                     IQLGVEIDGVFGSSAGAINAAAVASREYEAALRFWQELEVDTVFNISEKTSELLKKGF
                     AEWKFLEALSLAREVIDSRGIDSGPLETLLEKVLDEKRIRKSGIDMGIVVYSLSRMRP
                     LELSLQDIPEGLLAEFVSASANFPLFKRIEIESQIYIDGGIYNNVPVNMAIERGYSEI
                     ILVDLHYRRLKDFFDIWCAHGNEEIELTVISPQHDYVDLFEFNRSDLSRTLVQGYLDA
                     YSAMGKIFSGYTYIYISSSPGDFRKRFMSLRADQMKEALGLLGIAEAGEDGEHLWKSL
                     EKYMEEGGDVETAFLKVLDRFASLLGLERLHLYDTGELLGIISGEVDDDLTVSGRYNN
                     REYMQMGRFLRYIHENTRGDGSSLEIWSLITGTVESLSESSGELN"
     gene            6338..7678
                     /locus_tag="PLD15_00790"
     CDS             6338..7678
                     /locus_tag="PLD15_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB62461.1"
                     /translation="MFRFPGGLYSDVRIEDVFKTDISVTLDRLDNMKEQRYRAAFIRV
                     FDGRRWYYSSTTDIESIQRELERLAGLSSPDPNINNNKVVKTFEINKGSFLNFSGSKD
                     ISKTALKEKMDLLSQYFPHLKVSEPIKFWRGQYIDQRVVKSFYSSKGSALEFDYQRVG
                     FRLSYQMSLGEEKFMDRYDLAGNDISPLKDRGREVQERVKGSEHFLKNATTVKPGKYT
                     VVLSPEAAGVFAHESFGHKSEADFMLGDETMMREWSIGKKVGSDVLSIIDNGNHPGVG
                     FVSFDDEGTRARETYLIKDGYLTGRLHSVETAAALGEQPTGNARAVNFEYEPIVRMTT
                     TYISPGELTFDELLEGIETGIYVGSLNHGSGMSTFTLAPNQAFMIRSGKIAEPVRISV
                     ISGSVFQTLNEIEGITRDSKILSFSLGGCGKMEQYPLPVGFGGPYVRVRSLNVQ"
     gene            7688..8926
                     /locus_tag="PLD15_00795"
     CDS             7688..8926
                     /locus_tag="PLD15_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPB62462.1"
                     /translation="MTKERYMSHYVENAIKIVQTQVSSIRKKDIKKTSVRLYDGKCMG
                     VAGALGDQNEKDLLERAEQSLKLEVPYPFSPGRSMEREEDIESDLQRESELLEETEGM
                     LEALRETQPEFSFSQVFKQSTLETSIDNDLGLNLRSRRSFLNLTLVIKDRNSSNIMDA
                     VTGYAGRRFDREGFLNLTNMICDAYTKRIQSFEDGYYPVVFIADDTTYLTKFYEALHG
                     MLFGSGGSLFSGKLGEKLFSENFTLYQSRNAEDGFEGSFFDTEGSFNDGDLFPLVENG
                     VLKAPFTDKKSAALFNLPHTGSAGGEYDGIPELGFPKFKIKESEKTAKELLGGKKGIF
                     VLIASGGDFTPDGHFATPAQLAFLFDGERLVGRLPELNISSHLYEMFGNSFLGVSSNN
                     LLDLENSKVAVMRMKAEKII"
     gene            8971..9657
                     /locus_tag="PLD15_00800"
     CDS             8971..9657
                     /locus_tag="PLD15_00800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB62463.1"
                     /translation="MVFEEVEVVEHGYDRKKLEEEFDNLPEASPIADIDPFKGDIIAT
                     VGAIALDYLDLGRDDVLLDIGTGRGRWAVAASPHCKKVIGIDISKRMIEDARSNAENS
                     GVTNVEFYRGSFEDPSEEVDLKSKEINKVIAIYSLHHLTDAMKSETLTKFVSLIQRPG
                     TIVIGDIMWFDNPNNHRDEWDEVYYDDNETDHPAPIHFIREVFEKAGADEIEMVQVHP
                     LVGLICARFL"
     gene            9674..10084
                     /locus_tag="PLD15_00805"
     CDS             9674..10084
                     /locus_tag="PLD15_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB62464.1"
                     /translation="MSKLMRVVNREQCIGCYSCMSACSRTWENALTVEKSAMRVKNYP
                     GVEGAFSVRICYGCIEPDCAAACPSGALTPREGGGVKFEKDRCINCGACVKACVPRAL
                     QWDLEELRPIVCHHCGICAAFCPNNVLALVEVEK"
     gene            10081..11850
                     /locus_tag="PLD15_00810"
     CDS             10081..11850
                     /locus_tag="PLD15_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB62465.1"
                     /translation="MRQFWMLEIDLTDGKFKKVDVTGLFSEWLGGTGVATKLLYDNCY
                     PGLDPYSPQAPVIFAIGPLNNLFPVITKTVALFKSPLNGDLGESHAGGRLSLAMYESG
                     YHAILIRGRAPSLSYVVIENDTVYIKPCNSLKEMSALATERVLRDSEEGLGKRAIVRI
                     GPSGERLSPIACATVDTSRHFGRLGLGGVLGSKNLKAIVISGSKYWTLEKKREYNSLY
                     LRLYKEVVQSDSMAKYHDLGTSMNVYPLSRINGLPTRNFSQGFFEGADKISGEEFARS
                     RLSQQIACAGCPTGCIHMATLREPFNEPHHMYKTIKVSYDYELIYALGSNLSIDSTDM
                     ILKLLLYVEKQGWDAISIGVTLAWATEAFQRGLITERETDGVVLNFGDGEAYLKVLSR
                     IAAGKNQFFKDLERGADYCSKKYGGKDLSITFNSNEAPGYVTGPFAFIGYATGVRHSH
                     LDNAGYSLDQKAATRPVDFERTILEMYEEGVWRMILNSLVACLFSRKIYDINTIVESL
                     SAVGIDWSADRLTALSHRIHGLKYLFKERNGFTLDKLTLPEKLSRVYMTNGKVDEERF
                     RKGLELYGKLVEKDTQLPILDPD"
     gene            complement(11894..12874)
                     /locus_tag="PLD15_00815"
     CDS             complement(11894..12874)
                     /locus_tag="PLD15_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB62466.1"
                     /translation="MEQPEAVRLLLSSADEISGKSRKLDSSRVLFTGMGASLHAGSVA
                     TAFLRSRGIDSDSVEMSELISYSSPDLLKNYGTIVPISQSGESAELLRFMDDNIGLLD
                     KMALVTNNPLSTAGKRLAKEKVFPLHAGKERSMGATKTFTNSIVTTLLMALAWSGTVI
                     DFSKLPESIEEAFETDVTHLVELLAGKRERILVTRGYGIGVAKMARLMFAEVAKLSLV
                     FYSGAAFRHGPVELLNDRPVVMTMNPSGETAALMEELHRDIQQKCELITLTNFQSACE
                     RSIRVSVGLPEVIAPIPMMAILQKIANELALKRGFDPGKGIFGSKVTVKE"
     gene            13039..13848
                     /locus_tag="PLD15_00820"
     CDS             13039..13848
                     /locus_tag="PLD15_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB62467.1"
                     /translation="MSFLRTRDNRDIHYEVHGDCEKPPLMILNGIMMSTASWAGFIGD
                     FTKHFRLILMDFRDQGRSSRESAQYPIEIHVPDILSLLDELSIDKINLVGLSYGGQVA
                     QLLALESPGRLKTMILANTPARISPFLAELGEAWKEAAKLEDGERFFSLAIPFIYSDI
                     FYNNNLQWLKARQKVFKDLLDVAWFQGFIRLASSNPDFNVLDRVDGITCPTLLLCADR
                     DIITPLEEMKLIHERIKDSELLVIHNAGHGAFLEKTAEFITAVTGFILKNS"
     gene            14006..15052
                     /locus_tag="PLD15_00825"
     CDS             14006..15052
                     /locus_tag="PLD15_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HPB62468.1"
                     /translation="MIPLNGLPRIGIAGIGIYIPEKFTTARDLAMASGIPEDVIANKF
                     GVKRKPVPGTDDTTSHMGVMAALEALRDAQVDPMDVDLLIWNGAQHKDYPCWLAGLKV
                     ADVIGARRAWSFDMEAMCGSMMAGIDIAKSMMIAREDLNTVLLVSGYRNVDLIDLKNP
                     TTSFMLDLGASGAAVVLKKNHHSNIVLASAFKGDGSFSEDCVVPVGGSKSWPIKPEDI
                     SRFSFDVQGDAADFKKRLGETTMFNFYGVVRESLEKSGYSQSDIDYLAILHFKKSAHF
                     GVLEELGLSEDQSTYLDNYGHLGQNDQILSIKLGLKDEKIKDGDLVVMVGAGLGFVWA
                     ATTVKWGRDMEAEG"
     gene            15049..15795
                     /gene="fabG"
                     /locus_tag="PLD15_00830"
     CDS             15049..15795
                     /gene="fabG"
                     /locus_tag="PLD15_00830"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPB62469.1"
                     /translation="MRMEGKVVIITGAASGLGKASVEKFAKEGAITVACDMDEAGLEK
                     LREEFSSLPGKVVPKKLNVTDRSAISELVKLLKDEYGHIDGLVNNAGVTRDALLQRMT
                     EEDWDLVINVNLKGVFNMTQAVVPVMLDQGYGSIVNTSSIVGVYGNIGQSNYSASKGG
                     VVAMSKTWAKELTRKGAKIRVNAVAPGFIKTPMTDKVPEKLIQSLEEKIPLKRMGLPE
                     EVANLYFFLISDESTYITGQVIGIDGGLVI"
     gene            15792..16970
                     /locus_tag="PLD15_00835"
     CDS             15792..16970
                     /locus_tag="PLD15_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB62470.1"
                     /translation="MRISRSDRTKLQIKQAAEELFSRLGFELTSVANICRTCGLSNGA
                     FYRHYSGKEQVFKEIVHDIKTDFESALSTISGNREDERLFNSFRSVFDTLWNSKKKFM
                     AFHEAEYRFPEVENSVDSTYQEALIRALRIEGSTISMPKKWFLVGSSRFTAIYWIIFN
                     GVSVPDSTIESLVDFSMNGFIASPSDFKLNSLDLDLGNIDLSADSKAKDALLQSAEKL
                     IGEKGYFETSIYDITSHAGYGQGTFYLYFESKEKLLEELVLQANRRLRRTMRQASESI
                     KGRLNKEIRSYKAFLVFMNSHKELYNIVRESEFVLPETGRFYYEKLLTSYISALKESQ
                     DKGEIRNIDPRDLGVFLMGIGHFMGLDLLFVDNRPKDWDGYLKEIAALMTKGYGGVVN
                     "
     gene            16967..18265
                     /locus_tag="PLD15_00840"
     CDS             16967..18265
                     /locus_tag="PLD15_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPB62471.1"
                     /translation="MNWVEEYRKKKIGIDEALKNVKSNTKIVTSMAAMEAQGLLRNLH
                     RVENVENVAVATCLNIGEYPFFTLKEYEGRFLNESWFHSVGARKAVSSGLRTVTYIPN
                     NLHNAGVERIMANRPDIFWGVASPMDKNGYMTVSLSTVYERDMVEQAKMVIMEVNDRA
                     PKTHGDTHVHISEVDYVIENNFDIPELPDSEPSGTEMEIAGHISSLVADGSTLQIGIG
                     GIPNAVARLMEDKRDLGIHTEMFTESMIHLFEKGSITNRRKTLWPGKFIATFALGTRE
                     MYSFIEDNPGVLLLRGSYVNDPYVVAQNDNMVSINTAITVDLTGQVCSEAIGTRHYSG
                     TGGQLDTHRGASMARGGKGIIALRSTAKNGTVSTIVPLLPLGSPVTVPRQDIDYVVTE
                     FGVAHLKGLNVFQRVDALLNISHPDFRVELRKQASDLGLI"
     gene            18276..19475
                     /locus_tag="PLD15_00845"
     CDS             18276..19475
                     /locus_tag="PLD15_00845"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPB62472.1"
                     /translation="MKRVFIVGAKRTAIGTFGGTLKDTPAVELGIVASRAAIEQAGID
                     AGRIDEAIMGCILTANQGMGPGRQVSIGAGLPVESPGYLVNMLCGSGMKATMIGASDI
                     LTGEVGLVLTGGMENMSISPYMLDRARFGYRMGNGSIVDHMIRDGLTDVFNDYHMGVT
                     AENLAERHGIGRLEQDEFALESQKRAKEAIAAGKFKDEIATVVIKGRKGDTLFDADEH
                     PRETSMEILGKLKPAFKKDGTVTAGNSSGINDGGSATLLAGEEAVEKYGLRPLGEIVA
                     FAQAGVDPAYMGYGPVPAIEKALKKCGMNLNDIELLELNEAFAAQSLSVIKGLQELYG
                     VSKERLLERTNVNGGAIALGHPIGASGNRIIVTLLYEMAKRDLQFGLASLCIGGGMGT
                     ALVVKRV"
     gene            19587..20822
                     /locus_tag="PLD15_00850"
     CDS             19587..20822
                     /locus_tag="PLD15_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62473.1"
                     /translation="MKKAILLTLVLAVVAMMAVAATPIKIGAVVPLGDITGDQAAKAM
                     RLAVAEINAAGGLLGRPVELIVIDDELKPEKGAAAIEKLATVDKVDFFVGGMGSSVHL
                     AQIPVMKRYQKITIWIGAASYLCENAVGPDADWYFHLHPWDYQQGASYGLGWTELAEA
                     YPEIRTDRIFLAYEEGGFGTASHESYVALQRLAQQGEGAWAAIMNDYMGVSFKSAALG
                     GGDYRAMLQQAKTYNPDLFIWAGYDADALPMLQQAKEVGFAPEFFVGAPPGWPAHFGD
                     SPLAEGVTLYGMWAPTINKVSPAAEHFYNAYIEMFKEEPATYFAPLGYTNIYFLAEGI
                     KAAGTIDKAPLINALEKVEYASPIGEVLKIAPSNVIKHQGFTAQKILQWQGGEQQVIW
                     PLELKTADPTFPFPSWEGR"
     gene            20932..21822
                     /locus_tag="PLD15_00855"
     CDS             20932..21822
                     /locus_tag="PLD15_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB62474.1"
                     /translation="MFLILLIVAVAIWKPHAAIYGLQRGSLYALAGLPLSLTLGIVGI
                     MNLSHGDFLSLGIYIVYMFSVNTGMDPLVSILPVFILLFLLGAGVYKLTITKVLKAGH
                     LNQLLLTFGISMILLEVMNLIWTSRPRSVYVDYASSSVTVAGVRFGTYEFLYVVFAIA
                     VLIGLQLFLKKTRLGQASFAVGQNPRGAKIVGINTSFVYLIVFGISVAILGIAAGVMV
                     PRFAIFPSVGSSYTMKSFCLVAMAGLGNLTGILISGIALGVGEAIVQSIPGYAGWSDI
                     VFFGVLIAVIMIRSIRRPSL"
BASE COUNT         5947 a         5064 c         5836 g         4975 t
ORIGIN      
        1 tcgcccacag actgttgagc aagttcgaag gactagaggc actgtatctg ctggacagat
       61 acccggagaa actgtccggc ctggcctcag attccacccg ggtagtactc gcttccgaac
      121 cgtcggagat attcaaaaac tgcacacatg tgattctttc ggtgaaaccg caggactgca
      181 tggaactctt cgcccagatc aagaacggca ggcagcacaa tccgatcata ataagcacca
      241 taaccggcat tcagattgac accatcgttt ccaacacctc cataaaaaag attgcgagaa
      301 tcatgcccaa tgtccctagc gccatcggca ggggagtgac tggtctggtt ttttcggaag
      361 gtttcaccaa agacgagtgc cgggaagtca aaaggatact tttcacgatg ggagatatcg
      421 tcgaggtcag agaaaaggac ctggcggctg tcactgcgct cagcgggagc ggcccagctt
      481 tcgtatttgt aatcgtcgaa tcactcatag atgtaggatt gaagatggga ctctcctacg
      541 aggtctcaag agaactaata ctcggcaccc tgggcggatc ggtcgagctt ctcaaagaga
      601 ggggaaacca tccgggtgaa ttcagacacc tcgtcacttc gcccggaggt acaacgatag
      661 aggggattta ctcgctggaa agggaaggac tcaggggaac tataatgaag gctcttttcg
      721 atacttatct gagagcaaaa gagatcaacg ctgaactgga ggggaagaaa tgacggacct
      781 tgcggtaaaa ggagtgaaag tcaaggaagc ctccagattt ctgagactgg cgagcaccac
      841 cattaagaac caggctttga agtcgatcgc cggttcgata gacatgaacc gcagcgcgat
      901 actcgtcgcg aactctctgg atgttttgca ggccagaaac agagagatca aagaatcact
      961 gattgacaga ctcgcactca ctgaaaaaag gatagacggc atgatacagt cctgttatga
     1021 cgtagcggcg ttatccgatc ccgttggtga gatagaaacc acctggatcc agaaggacgg
     1081 cctgagaata tccagggaaa gggttcctct cggtgccata ggcatgatat acgaatcacg
     1141 gcccaacgtt acggtcgatg cctcgatact ggcactcaaa tccggaaact ccgttcttct
     1201 taagggcggc agcgacgcga taaactccaa cagggtgata gtgagtgcta taaaacgcgg
     1261 tctggagatg gtcggtcttc ccgaaggatg cgtagaattg atagaggacg tttccaggga
     1321 ttgtgtgatg gagatgttga aaatgagaga gtatttgtca ctgataatac ccagaggagg
     1381 tagcggtttg atacagttcg tggtcaacaa ctcgacagtg ccggttatcg agaccggaac
     1441 gggcaactgc cacatattcg tggactacaa cgccgatcta cgcaaggctg tatcgatcgt
     1501 tgataatgca aaaacgcaga gaccagggac atgcaacgcc gtagaaacga ttctggtaca
     1561 cgagaagatc gcgggggagt ttctcccgat gattaaagaa gctctctcaa gaaagaacgt
     1621 cgaactccgc ggttgtgaaa aatcgaggca gttcgtggaa atggtaccgg ccaccgaaga
     1681 agattatgag accgagttcc tggacctcat cctggccgta aaggttgtga gagatgtaaa
     1741 agaggccgtc gagcatatag agagatactc ctcacaccac tcggagtcaa ttctgactca
     1801 ggactactcg aacgccaggt actttttgaa ggccgtagat tcggccgcgg tttacgtgaa
     1861 cgcctcgaca cgcttcacag acggcggtga gttcggattt ggaagcgaga taggcataag
     1921 cactcaaaaa ctacacgccc gcgggccgtt cggtttgagg gaactcacga gctacaagta
     1981 caccgttatg ggtgactacc aggtgagaga ataatgtcca gaatagtgct taaagtcggt
     2041 tcaaacctgc tggtgaaaaa gaatggagaa ctggataaac attatatagc cgaactcgcg
     2101 cgagaaatag ccaatctcaa gtccctcgaa aatgaagtag ttctggtctc gtccggcgct
     2161 aaagccgccg gttttggcta tctcagcggt agaagcgccg agcacgatct gtaccttaaa
     2221 caggccctgt gcgcggccgg acaggtgcag ctgatgaagc tgtacgaaac ggttttcgat
     2281 ctctacggag aaaaggtcgc acagatactc gtcaaccgcg acgatttcgg tgatagaaag
     2341 cgcttcctca acctgaggaa taccctcata ggtcttctcg aaatgggtgt tatacccata
     2401 gtgaacgaaa acgacactac ctcgaccgaa gagatcatgt tcggcgataa cgacatactg
     2461 gctgccatgt tcgcgatggg ctggaaggcc gattttttaa tactcatgtc cacggtcgat
     2521 ggaatctacg acgaagaggg aaaggtgatg cgcattttcg actcagccag caagatcaag
     2581 agtatgtccg ggagcagctg ggggaccggg gggataggca ccaagatccg cgcggcaaga
     2641 ggggcttcgg cctcaggcat caaatgctgt atttgtaacg gccgcgaact gtcaaccatc
     2701 ggtcagttca ttcatggaat cgaaaccgga acggtcttcg aaccgatcga ttcgtcgccc
     2761 ggagccagaa agatctggat aggttttctc tcgactcctg gaggttcgct tataataaac
     2821 aaaggcgcca tcaccgcctt gaaaaggggt aagagtcttc tggctgtcgg cgtcgagaga
     2881 gtcgaaggcg agttctctca gggagaggta gtcaggatac tttccttcga gggtgaggaa
     2941 atcgcgcgcg ggataagcaa cttcagttcc ttcgaagtcg atagaatcaa aggaaccaga
     3001 agcgacaaac tatcggccat actcggccat gactgtgcga aagttgtcgt tcacatagac
     3061 aatatctacc aaaattgagc tttctactct ctcaccgcct ctctacgcga agcgaccgag
     3121 aatgtacaat tgagagaggg aggtgagaga aatgtacgtt gctgttatga ggtaccactt
     3181 tcacgaagag tctctcgaga gggcctgcag tatatggaaa gaagcggtgc tcgaaagggc
     3241 gaccggccgc gagggttttg tccgcgcctt catcatgacc ggtaccggcg gaaaggcatt
     3301 ggccgtgggt atgtgggaag aaaaaagaca cgccgacgag ttcatgaaaa caggcgtctt
     3361 caaggaactt ttgaatgact ttacagatat gatggtagcc aattccgagc acgaggccta
     3421 cgaaatgaag ttctgttcgg agaagtaacg atgctcgata aaatcgacct ggaagcaagg
     3481 ctaacgagta aggaatactc ccggaccctg gaatctctgg aactgcggct tggctttctc
     3541 cagcggaaac tcaaggagag atcggttccg gtggttatac tctttgaagg ctgggaggcg
     3601 tctggaaaag gtacggcgat aaacaatcta ataatgcccc tggacccaag agggtttaaa
     3661 gtatttccca tggccggcac tggggaaaac gataggtttt atccatatct ctggcctttc
     3721 tggaaccggc ttccctccgg tggccggata acgattttcg atagaagctg gtacaaagtt
     3781 ctgctcgatg aagaaaaagc gatgagcgaa agagagtttc tgtcgcattt gaaaaccttc
     3841 aactccctgg aaagactgct caccgattca ggcgccgtga tcatcaagtt ttttctgcac
     3901 atagagcgta aagagcaaaa gaagaggctt gagaagctgg attcagattc atcgacttcc
     3961 tggagggtga agaagagaga cttccgccag aacaagaact acgaaactgt gaagatgctc
     4021 gtcgagaagg tactgcaagc tagcgacacg gatatcgcac cctggtacat aataccctcg
     4081 accgatgaaa agctggccgc tgtacagata atgaacaccg tcgtgaactg cctcgaaaag
     4141 gctcttgaaa gagacaatga ggtaaccggc acggtcaatt cctggtccgg taacacttcc
     4201 gtcatcgatt cgctcgacct ctccaaaagt atagagaagg atctttacga gaagagactg
     4261 gccgaagcgc agaaaaaact gagggagtat caacatcttc tatacaaaaa gaggaagtcg
     4321 ctggtagtcg tttatgaggg tatggacgcc gcgggaaagg gtggaaatat aaggcgttta
     4381 accgctaaac tcgatccccg tggttacgag gtgataccga tcggtgcgcc taacgataca
     4441 gagaagtcgt accattatct ctggagattc tggaccaata tgccaaaaga cggacatatc
     4501 gctatcttcg accgcagctg gtacggccgc gttatggtcg aaagagttga aggcttcgcc
     4561 agtgaagcgg aatgggccag agcttaccgt gagataaacg atatggagga acagctacac
     4621 gatcacggaa ccgtcgtggt aaaattctgg ctgcatatag acctagatga acagttgaag
     4681 agattccagg cgagggaaaa cgatcccgaa aagcggtgga aaattacacc cgacgactgg
     4741 agaaaccgtg aaaaatggga tctgtataaa ccggcgatag aggagatgct cgttcgtacg
     4801 agtacttcgt acgctccgtg gaccgtagtg gagtcgaacg acaagagata cgccagagtg
     4861 aaagtcctgg agactctcgt gaaactgcta gaggagaacc tttgagtgaa aatatggact
     4921 accaaagtgt tatgtctgcg ttttttgttt cgatgtagtc ccggtgagtg cactatgctg
     4981 gagagaataa aatgaaagct attttgatta gcctgctctg tcaggcgata ctattttacg
     5041 gatatctttc ggcttcgctt gcgctggtgt tctccggagg cggggtccgc agcatttacc
     5101 agctcggagt ctggaaggct ttgatccagc tcggagtgga gatcgacgga gtcttcggca
     5161 gttctgccgg tgcgataaac gccgccgctg ttgccagcag ggaatacgaa gcggccttgc
     5221 ggttctggca ggagctagag gtcgataccg tcttcaatat atcggaaaag acatcagaac
     5281 tcctgaaaaa gggctttgcc gaatggaaat tcctcgaagc cctgtcgctc gccagagagg
     5341 taatagattc tcggggcata gactccgggc ctctagaaac tctgctggaa aaagtgctcg
     5401 acgaaaagag gatcaggaaa tcaggtatag atatgggcat agtcgtctat tcgttgagcc
     5461 gcatgaggcc gcttgaacta tccctccaag atataccgga aggtctgctc gcagagttcg
     5521 tttccgccag cgccaacttc cctctcttca aaaggatcga gatagaatct cagatctata
     5581 tcgatggagg aatttataac aacgtaccgg tgaatatggc gatagagaga ggctatagcg
     5641 aaataattct ggtggatctc cactacagaa ggctcaaaga ttttttcgat atctggtgcg
     5701 cccacgggaa cgaagagata gaactcactg tcatttcccc acaacacgac tacgtggacc
     5761 tcttcgagtt caacaggtcc gacctgtcta gaactctagt tcagggatat ctagatgctt
     5821 attcggccat gggcaagatc ttttcgggtt atacctatat atatatatct tcctcgcctg
     5881 gagatttcag gaagagattc atgtcgcttc gggccgacca gatgaaagaa gcgctcggcc
     5941 tgcttggaat cgctgaagcg ggcgaagacg gggagcatct gtggaagagt cttgaaaagt
     6001 atatggaaga gggcggcgat gtagaaaccg cctttttgaa ggtactcgac agattcgctt
     6061 cgcttctagg attggagaga cttcacctct acgacacggg agaactactg ggtatcatct
     6121 ctggtgaagt tgatgacgat cttacggtga gtggaaggta taataacaga gagtacatgc
     6181 agatgggcag atttctccgg tacatccacg agaacacccg gggagacggc tcctcgcttg
     6241 agatatggag cctgatcacc ggtactgtcg aaagcctttc ggaaagtagt ggagaattga
     6301 actgaaaaag atcgatatcg ctttaaggag gtttgaaatg ttcagatttc ccggaggatt
     6361 gtattccgat gtaagaattg aggatgtctt taaaacagac atctccgtaa cgctcgacag
     6421 acttgacaac atgaaagagc aacgctacag ggcagcattc attagagtct tcgacggacg
     6481 gaggtggtat tacagctcca caacagatat cgagtccatt cagagagaac tcgaaaggct
     6541 ggcaggactc tcaagtcccg acccgaacat caataacaac aaagtcgtga aaactttcga
     6601 aatcaacaag ggcagctttc tgaatttcag tggttcaaag gatatctcca aaacagccct
     6661 gaaagagaag atggatctac tgagtcagta cttcccccac ctaaaggtga gcgaaccgat
     6721 aaaattctgg agaggccagt acatagacca gagagtcgtc aaaagcttct attccagcaa
     6781 gggctcggca ctggagttcg attaccagag agtgggcttc aggctctcct accaaatgtc
     6841 cctcggcgag gagaaattca tggatcgtta tgatctggcc gggaacgaca tttccccgct
     6901 gaaagacagg ggacgtgagg tgcaggaaag agtcaaaggc tcggaacact ttctcaagaa
     6961 cgccacgact gtgaaaccgg gaaaatacac cgtagttctg tcgccggaag cagcaggagt
     7021 cttcgctcat gaaagcttcg gccacaagag cgaagccgat ttcatgctcg gcgatgaaac
     7081 gatgatgagg gagtggagca tcggaaaaaa agtgggcagc gatgtcctgt cgatcataga
     7141 caatggcaac caccccggtg taggcttcgt atctttcgac gatgagggta ccagagcacg
     7201 tgaaacttat ctcataaagg acggctatct caccggaagg ttgcacagcg tggaaacggc
     7261 ggccgcactg ggagaacagc ctaccggtaa tgcgagggcc gtgaatttcg aatacgaacc
     7321 tatagttaga atgaccacaa cctacatttc accgggagag ctaaccttcg acgaactcct
     7381 cgaaggaata gagacgggca tctacgtcgg ttctctcaac cacggatcgg gtatgtcgac
     7441 gtttaccctg gctcccaacc aggcttttat gataagaagc ggtaaaatag ccgaaccggt
     7501 caggatttcc gtgatatcgg gaagcgtctt ccaaaccctg aacgaaatag agggcattac
     7561 cagggactcc aagatactct cattctctct cggcggctgt ggaaaaatgg agcagtatcc
     7621 gttgccggta ggtttcggcg ggccgtatgt ccgtgttaga tctttgaatg tacagtgagg
     7681 tgttgagatg acaaaagaga gatatatgag ccattacgtt gaaaacgcga tcaaaatagt
     7741 acagacacag gtaagctcta taaggaagaa ggacataaaa aagacttccg taagactcta
     7801 cgacggcaaa tgcatgggag ttgccggtgc cctgggagac cagaatgaaa aggatctgct
     7861 cgagagagcc gaacagtcgc tgaaactaga agtaccttat ccattttcgc ccggccgatc
     7921 gatggaacga gaagaagata tagagtcgga tctccagaga gagtcggaac ttctcgaaga
     7981 aacggaaggg atgcttgaag cgctcaggga aactcaaccg gaattcagct tcagtcaggt
     8041 attcaagcag agcactctgg agacgtctat cgataacgat ctggggttga acctcagatc
     8101 ccgcagaagt tttctcaacc tgacactggt aataaaggat agaaattctt ccaacataat
     8161 ggacgcggta acaggctatg caggaagaag gttcgataga gagggatttc tgaacctcac
     8221 caacatgatc tgcgatgctt acacaaagag aatacagagt tttgaggacg gttactaccc
     8281 ggtggtgttc attgccgacg acacgactta ccttacgaaa ttctacgagg cgctccatgg
     8341 aatgcttttc ggaagcggag gttctctatt cagcggaaag cttggggaga aactgttctc
     8401 cgaaaacttc acgctctacc agagcaggaa tgccgaggat ggtttcgaag gttccttctt
     8461 cgataccgaa ggaagcttca acgatggaga tctcttccct ctagttgaaa atggcgtact
     8521 gaaagcgcct ttcacggaca aaaaaagcgc ggcgcttttc aaccttcctc atacgggatc
     8581 ggctggggga gagtacgatg ggatccccga actcggcttt ccgaaattta aaatcaaaga
     8641 gtccgaaaag accgcaaaag aactgctggg gggcaagaag ggcatcttcg ttctcatcgc
     8701 cagcggtggt gacttcacac cggacggcca tttcgcgacg ccggcccagc tggcctttct
     8761 cttcgacgga gaacgcctcg tggggcgttt gcctgagctg aatatcagct ctcacctcta
     8821 cgagatgttt ggaaatagtt tcctcggagt ctcgagtaac aacctcctgg atctggagaa
     8881 ctccaaagtc gcggtcatga gaatgaaagc cgaaaaaatt atttgaaact ggccgatact
     8941 caaaagggtt ataattatcg tgttaatcaa atggtatttg aagaggtgga agtggtggaa
     9001 cacggttacg atcgcaaaaa gctggaagag gagttcgaca accttcccga agcatctccc
     9061 atagcagata tagatccatt caagggtgac atcatagcca cggtcggtgc gatagctctc
     9121 gattatctcg acctaggtag agatgatgtt ttgctggata ttggcaccgg acgtggtaga
     9181 tgggccgttg ccgcgagccc tcactgcaag aaagtcatcg gtatcgatat aagcaagaga
     9241 atgatcgaag atgccaggtc gaacgccgag aattccggtg ttacaaacgt cgaattctac
     9301 aggggatctt tcgaagatcc ttcagaagaa gttgacctaa aatctaagga gataaacaaa
     9361 gtcatagcga tctattcgtt gcatcatcta accgacgcta tgaaatccga aaccttgacc
     9421 aaattcgttt ccctcatcca gagacctgga actattgtca taggcgacat aatgtggttc
     9481 gacaatccca acaaccaccg tgacgaatgg gacgaagtct attacgacga caacgaaacg
     9541 gaccatcccg cgccaataca tttcatcaga gaagtcttcg aaaaggccgg ggcagacgag
     9601 atagagatgg tccaggttca ccctctggtc ggcttgattt gcgcccgctt tctctgatct
     9661 gggagatgag atcttgtcga aactcatgag agtggtaaat cgagaacaat gcataggctg
     9721 ttacagttgt atgagcgcct gttccagaac gtgggaaaat gcgctcacgg ttgaaaagtc
     9781 ggccatgaga gtcaagaact atcccggagt agaaggagcc ttttcagtca ggatatgtta
     9841 cggctgcata gagccggatt gcgcagccgc ctgcccttcg ggagccctaa ctcctagaga
     9901 gggaggcggg gtgaaattcg aaaaagacag atgcatcaac tgcggcgcat gcgttaaggc
     9961 ctgtgtaccc agggcgcttc agtgggatct ggaggagctt cgtcccatcg tctgccatca
    10021 ttgtggcatt tgcgctgctt tctgcccaaa caacgttctg gcgcttgtgg aggtggagaa
    10081 gtgagacaat tctggatgct cgaaatcgac ctgaccgacg gaaagttcaa aaaggtcgat
    10141 gtcacgggat tgttctccga atggctcggg ggaacaggcg tggccaccaa actcctctat
    10201 gataactgtt atcccgggct cgatccatac tcccctcagg caccggtgat tttcgccatc
    10261 ggacctttga acaatctttt tccggtgatc acaaagaccg ttgcactttt caaatctcca
    10321 ctcaacggag atctgggcga atcccacgcc gggggaagac tcagtctggc gatgtacgag
    10381 agcggttacc acgcgatact tatacgcggg cgggctcctt ctctctcgta tgttgtcata
    10441 gaaaatgata cagtatatat aaaaccgtgc aattctctca aagagatgtc ggctctagca
    10501 acggagagag tcttgagaga cagcgaagaa ggactcggca agagagcgat agtgcgaatc
    10561 ggcccatcgg gtgaaagact atcgccaata gcctgtgcca cagtcgatac ctcgcgtcac
    10621 ttcggacgac ttggacttgg aggcgttctc ggctccaaga atcttaaagc catagttatc
    10681 tcgggcagca aatactggac tctggagaag aagagagaat ataattccct ttatttgaga
    10741 ctgtataaag aagtagttca gtcagattct atggcaaagt atcatgatct gggtacatct
    10801 atgaacgtct atccgctaag caggatcaac ggacttccaa cgcgaaactt ttctcagggt
    10861 ttctttgaag gagccgacaa gatatccggt gaagagttcg cccgatcaag gctgtcccag
    10921 caaatcgcct gtgccggctg tccgacaggc tgcattcaca tggccacatt gagggaaccc
    10981 ttcaacgagc cccatcacat gtacaagacc ataaaagtct cgtacgatta cgaactgata
    11041 tacgccctgg gctcaaacct ttcgatcgat tctacggata tgatactgaa actacttctg
    11101 tacgttgaaa aacaaggatg ggacgccatt tcgataggtg tcactctcgc ctgggcgacc
    11161 gaagccttcc agaggggact gataacggaa agagaaaccg acggtgtagt gttgaatttc
    11221 ggcgatgggg aggcttattt gaaagttctg tcgaggatag cggccggtaa aaatcaattc
    11281 ttcaaagacc ttgaaagggg ggccgattac tgttcgaaga agtacggcgg aaaagatctc
    11341 tccattacat tcaactccaa cgaggcgccc ggttatgtca ccggtccatt cgctttcata
    11401 ggttacgcca ccggtgtccg tcattcacat ctggacaacg ccggttattc tctggaccag
    11461 aaggcggcca ctaggcccgt ggatttcgag agaacgatcc tggaaatgta cgaagagggt
    11521 gtatggagga tgattctcaa ctcgttagtc gcctgtctct tctcaaggaa aatctacgac
    11581 attaacacga tagtcgaatc attaagtgcc gtaggaatag actggagcgc tgacagattg
    11641 acggcccttt cccacaggat tcacggtctt aaataccttt ttaaggaacg gaacggattc
    11701 acgctcgaca aactcaccct gccggagaaa ctctcccgtg tatatatgac caacggaaag
    11761 gttgacgaag agagattcag aaaggggctc gagctgtacg gaaagcttgt cgaaaaagat
    11821 acgcaactgc cgattctgga cccggattga tccaggtcca gaatcaacac gtatgaactc
    11881 agtcgataac tccttattct ttgaccgtca ccttcgagcc gaatatgccc tttccaggat
    11941 cgaagccgcg tttcaacgcc agctcgttgg cgatcttttg aaggatggcc atcatgggaa
    12001 tcggagcgat gacctcggga agcccgaccg acaccctgat gcttctctca caagcggact
    12061 ggaaattggt cagagttatc agttcgcatt tctgttgaat atctctgtga agctcttcca
    12121 ttaaagcggc cgtttctcca gacgggttca tagtcatgac gacgggtctg tcgttcaaca
    12181 actccaccgg gccgtgtctg aaggccgcac cggaatagaa aacgagactt agtttcgcaa
    12241 cctcggcgaa catgagtcgg gccatcttcg ccacgccaat tccatagcct cgtgttacga
    12301 gtatcctctc cctcttgccg gcaagcagtt cgaccagatg ggttacatct gtttcgaagg
    12361 cctcttcgat cgattccggc agcttggaga agtcgatcac ggttcccgac caggccagag
    12421 ccatgagaag agtggtcacg atgctgttgg tgaaggtctt cgtcgctccc atcgatctct
    12481 ctttgcccgc atgaagcggg aaaacctttt cttttgcgag tcttttgccg gcagtactca
    12541 acggattgtt ggttacgagg gccattttgt cgagaaggcc gatattatcg tccatgaatc
    12601 tcaacaactc tgcactctct cccgattgac ttatcgggac gatcgtaccg taattcttga
    12661 gaaggtccgg ggaggaatag gaaatcagct cggacatttc cacactgtcg ctatctattc
    12721 cccgggaacg tagaaaagcc gtcgcaacgc taccggcatg aagcgaagct cccatgcccg
    12781 tgaaaagaac cctcgagctg tccagtttcc gggattttcc gcttatctcg tctgcggaac
    12841 tcaagagaag tctaactgct tcgggctgtt ccataaaatc ttgcttgaat tgtgacatca
    12901 aacccatcct ttcagaagtt atttatcgct ggaaggtgct atctatttcc atgctcatgc
    12961 ttcatttcaa tatcagttta ccaccacatc cgggaaaaaa ccgaccggaa gtgtaataat
    13021 cgttgaggag gtgaggtaat gtcgtttctt agaactcgag acaataggga tatccattac
    13081 gaagttcatg gggattgtga gaaaccgccc ttaatgatac tgaacggaat catgatgtct
    13141 accgcttcat gggcagggtt tattggggat ttcacgaaac atttcagatt gattcttatg
    13201 gatttcagag accagggaag atccagcaga gagtccgcgc agtaccccat cgaaatacat
    13261 gtcccggaca ttttgtctct tctggacgag ttatccatag acaagataaa cctcgtggga
    13321 ctctcctacg gtggacaggt cgcacaacta cttgcccttg aatcgcccgg aaggttgaaa
    13381 acgatgatac tggctaatac accagccaga atctcccctt ttcttgccga acttggagag
    13441 gcctggaagg aagcggccaa acttgaagat ggcgagagat tcttctctct ggcgattccc
    13501 ttcatttact ccgatatctt ttacaacaac aacctccagt ggctcaaagc tcgtcagaaa
    13561 gttttcaaag acctcctgga tgttgcctgg ttccagggat ttataaggct ggcctcttcc
    13621 aatccggact tcaacgtcct ggatagagtc gacgggataa cctgcccgac gcttctactg
    13681 tgtgccgaca gagatataat aacccctctg gaagagatga agctgatcca tgaaaggata
    13741 aaggactcgg aattgctggt cattcacaac gcaggtcacg gcgcatttct tgaaaagaca
    13801 gccgaattca taacggccgt cacggggttt atattgaaga attcttgaat tttactttca
    13861 taatcatctt tattcactcc tcatcgcctg cagttgcatc aggtcatctc tgattgtgtt
    13921 tactaaagaa tgttaaatat agtatgctga aagtgagaga tgattcactt gtcataaatg
    13981 agagataatt cacaaaggtc gagaggtgat cccattgaac ggtcttccga ggatcgggat
    14041 agcgggtatt ggtatctaca tacccgagaa gtttacaacg gcgagagacc tggctatggc
    14101 gtcgggaatt ccagaagatg tcatcgcgaa taagttcggt gttaagcgga aacccgttcc
    14161 agggactgat gacacgacca gtcacatggg agtcatggcg gcgcttgaag ccttacgtga
    14221 cgcacaggta gatccgatgg acgttgatct cctgatatgg aacggcgcac agcacaaaga
    14281 ctatccctgc tggcttgcgg gactcaaggt cgcagacgtc ataggcgcca gaagggcatg
    14341 gtcgttcgat atggaggcca tgtgcggttc gatgatggcg ggaatcgata tcgccaagag
    14401 catgatgatc gcccgcgaag atctcaatac cgttctgctc gtgagtggtt atagaaatgt
    14461 ggatctgata gatctcaaaa accccactac ttccttcatg cttgatttag gtgcgagcgg
    14521 agccgcagtc gttttgaaga agaaccacca cagcaatatc gttctggcat cggccttcaa
    14581 aggcgacggt tctttttcgg aagattgcgt ggtgccggtt ggagggtcta agagctggcc
    14641 aatcaaaccc gaagatatat ccaggttttc cttcgatgtt cagggagacg cggcagactt
    14701 caaaaaacgc ctcggcgaga cgaccatgtt caatttttac ggagtggtaa gagagtctct
    14761 cgagaagagc ggctactctc agagtgatat agattatctt gctatactac atttcaaaaa
    14821 atccgcccac ttcggagttc ttgaagagct cggtttgagt gaagaccaga gcacttacct
    14881 cgacaactac ggccacctcg gccagaatga tcagatactt tccataaaac tcgggctgaa
    14941 agacgagaag atcaaagacg gggatctggt agttatggta ggtgcaggac ttggattcgt
    15001 atgggctgca acaacggtga agtggggcag agatatggag gctgaaggat gagaatggaa
    15061 ggtaaagtgg ttattattac cggtgcggcc agtggactgg gaaaggcgtc ggtggaaaaa
    15121 tttgcgaaag aaggcgctat aacggtcgcc tgcgatatgg acgaggctgg tctggaaaag
    15181 ttaagggagg agttctcttc tcttcccggc aaagtagtac ccaaaaaact taacgtgact
    15241 gatcgttcag cgatcagcga gcttgtcaaa ttactcaagg atgagtatgg tcacatagac
    15301 ggcctggtaa acaacgcagg tgttaccaga gatgcactcc tgcagagaat gaccgaagaa
    15361 gactgggatc tggtcatcaa cgtcaacctg aagggggttt tcaacatgac acaggccgtg
    15421 gtccccgtca tgctggatca gggatacggt tcgatagtta acacctcttc catagtcggt
    15481 gtgtacggca acataggtca gagcaactac tcagcttcta aaggcggtgt tgtcgccatg
    15541 tccaagacat gggccaagga attgacgaga aagggcgcca aaatcagagt gaacgccgtg
    15601 gctcccggct tcataaagac acccatgaca gacaaggtcc ccgagaagct aattcagtct
    15661 ctcgaggaga agatacccct gaagagaatg ggactaccag aagaagtggc caacctttac
    15721 ttcttcctga tatccgatga atccacctac atcactggtc aggtgatagg aatagatgga
    15781 ggcctcgtga tttgaggatc tcacgcagcg atagaactaa actccagatc aagcaggcgg
    15841 ccgaagagtt gttcagccgc ctgggtttcg agttgacgtc tgtcgccaat atttgtagaa
    15901 cctgtggcct ctccaacgga gccttttaca ggcattattc cggcaaggaa caggtcttca
    15961 aggaaatcgt gcatgatata aaaaccgatt ttgaaagcgc tctcagtacg ataagcggta
    16021 atagagaaga cgaaaggctt ttcaactctt tcaggtctgt cttcgatacc ctctggaatt
    16081 ccaagaagaa gttcatggcc ttccacgagg cagagtatag atttccagag gttgaaaact
    16141 ccgtcgatag cacatatcag gaagctctca tcagggcttt aagaatcgag ggatcaacta
    16201 tctctatgcc aaagaagtgg tttctggttg ggagctccag attcacggcc atctactgga
    16261 taatcttcaa cggcgtcagc gttccagact cgacgataga atcgctggtc gatttttcga
    16321 tgaacggttt catcgcttcc ccgtcggatt tcaaactgaa ctctctggat ctcgacctcg
    16381 gaaatataga tttatcggcc gatagtaagg ccaaggatgc gctcctgcaa tctgccgaga
    16441 aattgatcgg agagaagggg tatttcgaga cgtcgatcta cgatatcacc agtcacgccg
    16501 gatacggcca gggaaccttc tatctgtact tcgaaagcaa agaaaagctc cttgaagagc
    16561 tggttctcca ggccaacaga agacttcgcc gaaccatgag acaggcctct gagagtataa
    16621 aggggaggtt gaacaaagaa ataagatcgt acaaggcctt cctggtattc atgaattcac
    16681 acaaagaact ctacaacata gtcagagaat cggaattcgt acttccagaa acgggaagat
    16741 tctactatga gaaactgctc acttcctata taagtgctct aaaagaatca caagataagg
    16801 gcgagataag gaacatcgac ccaagagatc ttggtgtctt tcttatggga atagggcatt
    16861 tcatgggact ggatcttctc ttcgtagata acagaccaaa agactgggat ggatacctca
    16921 aagaaatagc agcactcatg acaaagggct atggaggtgt tgttaattga actgggttga
    16981 agaatacaga aagaagaaaa tcggtataga cgaagctttg aagaacgtga aatcaaatac
    17041 gaaaatagtc acaagtatgg ctgcgatgga agctcaagga ctgctgcgaa accttcacag
    17101 ggtggaaaat gtggagaacg tcgcagtagc gacctgtttg aacataggcg agtacccctt
    17161 tttcacactc aaagagtacg aaggcagatt cctcaacgaa tcctggttcc actcggtcgg
    17221 tgccagaaag gctgtcagca gcggattgag aacagtaacc tatattccca acaaccttca
    17281 taacgcaggg gtggaaagga taatggccaa cagaccggat atcttctggg gagtggcctc
    17341 tccgatggat aaaaacggtt acatgaccgt ttctctctcc acggtttacg aacgcgacat
    17401 ggttgaacaa gcgaagatgg tcatcatgga agtcaatgat agagccccga agacccacgg
    17461 cgacacgcac gttcacataa gcgaggtaga ttacgtcatt gaaaacaact tcgacatacc
    17521 cgaacttccc gacagcgagc cttccgggac agaaatggag atcgccggtc acatttcctc
    17581 tctggtggcc gatggctcca cccttcagat aggcatagga gggattccaa acgcggtcgc
    17641 cagattgatg gaggacaagc gtgacctcgg tatacacacg gagatgttta ccgaatcgat
    17701 gatacatctc ttcgaaaagg gttccataac caacaggagg aagaccctct ggccgggaaa
    17761 attcatcgcc acttttgccc tgggaacaag agagatgtac tccttcatcg aagataaccc
    17821 cggagtgctt ctcctcaggg ggagttacgt caacgatcct tacgtggttg cccaaaacga
    17881 caacatggta tcgataaaca cggcgataac cgtggatctc actggccagg tctgttccga
    17941 agccatagga acgcgtcatt acagcggtac gggcggtcag cttgatacac acagaggggc
    18001 ctccatggcc cggggaggaa agggaataat agcgctgcgc tctacggcga agaacggaac
    18061 ggtatctaca atcgttccgt tgttgcctct gggctcgccc gtaacagttc caaggcagga
    18121 catagattat gtggtaacgg agttcggtgt ggcccatctt aaagggctga atgtcttcca
    18181 gagagtggat gcccttttga acatatcaca tcccgacttc agggtcgaac tgcgaaaaca
    18241 ggccagtgat ttaggtttga tataggaggt attcgatgaa gagagtgttc atagttggag
    18301 ctaagagaac ggctatcgga actttcgggg gaaccctcaa ggacacaccg gccgtggagc
    18361 tgggaatcgt cgcttccagg gccgccatag aacaggccgg tatcgatgcg ggcaggatcg
    18421 atgaagcgat aatgggctgt attctcaccg ccaatcaggg aatgggaccg ggaagacagg
    18481 tttcgatagg tgccggactt cccgtcgaga gtcctggtta cctggtgaat atgctgtgcg
    18541 gttcgggtat gaaggctacg atgatcgggg cctccgatat cttaaccgga gaggtcggac
    18601 tggttctgac tggtggcatg gagaacatgt cgataagccc gtacatgctg gacagggcca
    18661 gatttggtta cagaatgggc aacgggagta tcgtggacca tatgataagg gatggcctta
    18721 ccgatgtttt caacgattat cacatgggcg tgacggccga gaacctggcc gagagacacg
    18781 gtatcggtcg ccttgaacag gacgaattcg ccctcgagag ccagaagaga gccaaggaag
    18841 ccattgcagc gggcaaattc aaagatgaga tagccaccgt ggttataaag ggaagaaaag
    18901 gcgatacgct cttcgatgcc gacgaacatc caagagagac cagtatggag attctcggca
    18961 agcttaaacc ggctttcaaa aaggatggca cggtaacggc cggcaattca tcgggtatca
    19021 acgatggagg tagcgcgacg cttctggcag gcgaggaggc ggtcgaaaag tatggattga
    19081 gaccactcgg cgaaattgtc gcctttgcac aggccggagt cgatccagcg tacatgggat
    19141 atggtccggt ccctgcaatc gaaaaagctt tgaaaaagtg tggaatgaac ctcaacgata
    19201 tcgaactcct cgagttgaac gaggccttcg cagcgcagtc tctatcggtc atcaaaggat
    19261 tgcaggaact ctatggggta tcgaaagagc ggctgttgga gagaacgaac gtcaacggcg
    19321 gcgcgatagc tctcggacat ccaataggtg cttcgggaaa cagaataata gttaccctcc
    19381 tgtatgaaat ggccaaaaga gacctgcagt tcgggctcgc ctccctttgt atcggcggtg
    19441 gcatgggaac cgcgttggtt gtcaagagag tctaatgaaa gaaatattgt ctatcacctg
    19501 gttcatagat ctcctcaaac gttctggcgt gtggatttat cgcaattggc aattctagaa
    19561 tgaactacct attaggaggt gggaaagtga aaaaggcaat attactaaca cttgttctcg
    19621 cggtagtagc gatgatggct gttgccgcaa ccccgatcaa gatcggtgcc gtggtacccc
    19681 ttggagacat aactggtgac caggctgcca aagccatgag actggcagta gccgagatca
    19741 acgccgccgg tggtcttctt ggtagaccag tcgaactgat cgtgatcgac gacgaattga
    19801 aaccggaaaa aggcgccgcc gcgatcgaaa aactcgcaac tgtcgataag gtagatttct
    19861 ttgtcggcgg tatgggttct tctgtacact tggcacagat ccccgtcatg aagagatacc
    19921 agaaaataac catctggata ggtgccgctt cctatctctg tgaaaatgca gtgggaccgg
    19981 atgccgactg gtatttccat cttcatccgt gggattatca gcagggtgcg agttacggac
    20041 tcggatggac ggaactggcc gaggcctacc ctgaaatcag aaccgacagg atcttcctcg
    20101 cgtacgaaga gggaggtttc ggaacggctt ctcacgaatc gtacgtcgca ttgcagagac
    20161 tggcacagca gggtgaaggt gcctgggcgg cgatcatgaa cgattacatg ggcgtatcct
    20221 tcaaaagcgc cgcgcttggt ggcggagatt acagggccat gctccagcag gccaagactt
    20281 acaatcccga tctcttcatc tgggccggtt acgacgcaga tgccctacca atgcttcagc
    20341 aggccaagga agtcggattc gctccagagt tctttgtggg agcacctccg ggctggccgg
    20401 cacacttcgg tgattctcct ctggccgaag gcgtgacgct ttacggaatg tgggcaccca
    20461 cgattaacaa agtgagcccg gcggccgagc acttctacaa cgcctacatc gaaatgttca
    20521 aggaagagcc tgcgacatac ttcgcaccgc tcggttatac caacatctac ttcctcgcgg
    20581 aaggtatcaa ggccgcagga acgatcgaca aagctccttt gatcaatgct ctggaaaaag
    20641 tcgaatacgc ttcacccatc ggtgaagtgc tgaagatcgc tccgagcaat gtaatcaagc
    20701 atcagggttt cacggctcag aagatacttc agtggcaggg cggcgaacag caggtaatat
    20761 ggccgctcga gctcaagacg gcagatccga cgttcccgtt cccgagctgg gaaggaagat
    20821 agtttgatcc gaggtcgggc ttcggcccga ccttattatc tcaaattggc aagaaccggg
    20881 gtggtgacag acttggagag catctctttt tggaagaaat ataagaatat cttgtttttg
    20941 atcttgctga tagtcgccgt tgcgatatgg aaaccccatg cggccatcta tggtttgcaa
    21001 aggggcagtc tttatgctct tgccggcctg cctctttccc tgacgctggg gatagtgggt
    21061 ataatgaacc tctcacacgg ggatttcctt tctctgggaa tatacatcgt ttacatgttt
    21121 tccgtgaata cgggaatgga tcctctcgtc tcgattcttc cggtctttat cctgctcttt
    21181 ttactgggag cgggtgtcta caaattgacg ataacgaaag ttctgaaggc cggtcacttg
    21241 aatcagctgc tccttacctt cggaatttcg atgatccttc tggaagtgat gaacctcatc
    21301 tggacatcca gacccaggag cgtttacgta gattacgctt cttcctctgt cactgtcgcg
    21361 ggcgtaaggt tcggaactta cgagttcctt tatgtagtct tcgccatcgc cgtgctcata
    21421 ggtttgcagc tgtttctaaa aaagaccaga ctcgggcagg cttctttcgc ggttggtcaa
    21481 aatccgcgtg gggccaagat agtcggtata aatacgagtt tcgtttacct tatagtcttc
    21541 ggcatatcgg tggcgatcct tggaatagcg gccggtgtaa tggtaccgag gttcgcgatc
    21601 tttccttccg ttggctcttc ttatactatg aagtctttct gtttggtggc tatggccgga
    21661 cttggaaatc tcacaggcat actgatcagt ggtatcgctc tgggggtagg tgaagcgata
    21721 gtgcagtcca ttcccggtta cgccggctgg tccgatatcg tcttctttgg agtactgata
    21781 gccgtcatca tgataagatc gataaggagg ccttcactat ga
//
LOCUS       DAOOLY010000008        21151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_2466_length_21151_cov_5.247251, whole genome shotgun sequence.
ACCESSION   DAOOLY010000008 DAOOLY010000000
VERSION     DAOOLY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 21151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21151
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2466_length_21151_cov_5.247251"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLD15_00860"
     CDS             <1..211
                     /locus_tag="PLD15_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62475.1"
                     /translation="YHQTATAIFTNGLSLIAGFSVLLLTPLLLYVDVAKLMVSGLAVG
                     MIFTLVLLPEIAARRWKQNNRHSSG"
     gene            244..795
                     /locus_tag="PLD15_00865"
     CDS             244..795
                     /locus_tag="PLD15_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HPB62476.1"
                     /translation="MKALVTLLPDPISEMVHLIWNELERDFNFRSVLVTPYPHFTYCV
                     VEDFTEGLYEKIEATITNVDSFTVRTEYLGVFSGESPVVYIGIARIPELSHFHDTLWR
                     RFFGPERDIHDLYGIAHWIPHITLIFGRDLNRENFCPAMERLVFRDFHREFEVNNLAI
                     LSGSLESVIEVEKIFYFPGNQHP"
     gene            876..3224
                     /locus_tag="PLD15_00870"
     CDS             876..3224
                     /locus_tag="PLD15_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB62477.1"
                     /translation="MRKLNWKEIDLKVTLPRKVKSAKSIDGLEEFIGQERAIKALETG
                     LKIKARGYNIFVSGSTNTGRRTFVSRYLKKKAEGGETPGDWIYVYNFDDPRSPNSIPL
                     KAGTGKSFQKEMGEFVEITINAISESFQSEDYQQKMSAIQSEQAENRSNLLKELIDKA
                     RERDFTVQINQTGVATIPLWNGKPLTQEIYEALPEEYQKTISKKGEEVRELVNSYILR
                     ISKMEKEYGEKFKELNRDVASFALEGHIKEMKDKFSENKEAIEFLDNLRSDLLDNLGV
                     FFSQEIDAKNFFGKRYAINLFVDNSGINGKPIVEVTNANYSSLFGRIEYLAKMGMLDT
                     DHSMIRSGAIHRSNGGFLVLDAKSVLSEPYVWQTLKQLLFSGSEVIENLEHKVGLLST
                     VSLKPEPIPLDMKIIMIGEPWIYSMLSTMDTDFKKLFKIKAEFDWEMRLDGDTVGKLC
                     RLICSIARDNSLMDFDRDAVREVIKKAIILSGNRKKVSTQFGALKQLLLESSAVVEIN
                     GGEMVSAEDVSRAWEEMKTRVSLYQDKVKEMFADSTLLVQTEGELIGEINGLTVVQTE
                     DLSFGIPVKITAKAGPGNSGIVDIQKESELSGNIHDKAGLTIQGYMNSKYAREFPLSL
                     NGSISFEQVYSMVEGDSASVAETVAFLSAIAEAPIKQSIAVTGSINQSGRVQPVGGVQ
                     FKVEGFYELCKLKGLNGKQGVVIPDTNFDNLVLPDEIVLAIKNGRFNIWTVETVDEAI
                     ELLTGLKAGQPDGDGKYPEGTFNNLVLQRLERFYEISMQEKK"
     gene            3692..5344
                     /locus_tag="PLD15_00875"
     CDS             3692..5344
                     /locus_tag="PLD15_00875"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPB62478.1"
                     /translation="MSSSSRFPRSSIGKSNPLFSQTRTTVETAFYGNNVSTVISPCEA
                     YKLAKSSPGTIVTDMPVYKPEKLGLEPDSKVLIFNDGAVTGRCASARRILGEPGVSEA
                     EYGAKIREAVYSTRKKKMYHAQGYIGLDRDFMIKAHLLIPENHENIMYNWLLNFQPMN
                     EFYNEMYENSKKYEGEGEIFVFSDPDWQHPEHPLGLSFFDPEHNCAAILGMRYFGEFK
                     KGTLTLAWGCANRHGFAACHGGQKRYNLPDRKYVAGFFGLSGSGKSTLTHAKHGGKYD
                     VTILHDDAFVISSENGSSVALEPSYFDKTSDYPLCSDDNKYLLSVQNVGATQDENGNI
                     VIVSEDIRNGNGRAIKSRLWSPNRVDRFDEPVDTIVWLMKDPSIPPVVKIEDPVLSSA
                     MGATLATKRTSAERLAPGVDPNALVIEPYANPFRTYPLSDDFKRFLELFQKRNIDCYI
                     FNTGHFRERKIPKELTLNILENIVEGKARFKKWEPFEELEIMELEGFTPDANDGDYMK
                     LLRDRLVDRFNFIKSRESERGGFDRLPPEAIEVFAKLIERLR"
     gene            5524..6204
                     /locus_tag="PLD15_00880"
     CDS             5524..6204
                     /locus_tag="PLD15_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62479.1"
                     /translation="MTVPVVLEEFINRFKGWISGSGDLTELVGAFSSLSTYTIIAFVA
                     IGLSLLIFGKKLFAVFYAVLWTVIGSHVGYLWGQGMETLYTIELMILIGVLLPLVAIK
                     AREIFVFTMVLFGTGFFATYIAQSLGTFQSLTILDPPFVLAGCLLGWLAVRFMDYVMI
                     VSTSLIGAFLLMLAVSYFFRDITDLLTMLLVFLATSLFGIFLQMDVYLKNKKSSKKKK
                     NRSKGAKR"
     gene            6201..6794
                     /locus_tag="PLD15_00885"
     CDS             6201..6794
                     /locus_tag="PLD15_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB62480.1"
                     /translation="MIRIILSDRMEENCYLVEDENGIVVIDPGIGAADRIIDLVGDTK
                     LSILITHGHADHIFDIDRLKGDNIFVHPLDRERLLDAEKSLLNFFGRGPLKIDESTIV
                     DCSQIGGKWRVLHTPGHTPGSCCYLYDDRILFTGDTVFMQSVGRTDLPGGSEETMIGS
                     LHLLKEIFENGPDLKVFPGHGPGTDAREILTDNPFFR"
     gene            complement(6787..8079)
                     /locus_tag="PLD15_00890"
     CDS             complement(6787..8079)
                     /locus_tag="PLD15_00890"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB62481.1"
                     /translation="MCDTFVALGSSTPDGRTIFAKNSDREPNEPQYAFFYPRTEYSGK
                     ELYTTSQKIKQVEKTYAVLISKPSWMWGGEMGVNENGVAIGNEAVFTREMVSRQGLLG
                     MDILRIALERSESAEQAVTVITDLIQKYGQGESAGYTKTIFYHNSYIVADRENAWVLE
                     TSDRYWTADRVKDRASISNCLTIAGPGELQHPETASNALEKGWCKKAEEFRFKKCYEK
                     KLFSWLSGGEARRSRTMNLLGEKDVDLKRAFEMLRDHGGSRFFGSMKNVCMHSGTGLV
                     NSQTTGSMVVVLGEKIDVWVTNTSAPCLSFYKPVWFDSNSSTLPFEKEADALNHWGNW
                     EIFHRLATTRYEKARQLWEQYCLPFEEELILHKDSLEHREITAQAFDTGWKLAKKMAS
                     LLQESPVETGLFNRRYWKKKERELQELKTKNFKRPGSI"
     gene            complement(8092..8856)
                     /locus_tag="PLD15_00895"
     CDS             complement(8092..8856)
                     /locus_tag="PLD15_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPB62482.1"
                     /translation="MRLFYLEKILSSILTPPGLVILILLILALTLLRKKDARGIRAGG
                     IVLLILSFFGYLLSTGLGTYLYLRPLEKEYQLPKSVSGQVIVVLSSGVIITPVGQVLD
                     NHSVARLSAALNLHLQTKLPIVVTGSFVPGRNTLPVAELMRDWLLGQGVGTSRILVEP
                     QARNTWENAEYTARLCSQSGWKNVFLVTSAVHMKRAVKSFEEFGLIVTPYPTDYLYDH
                     TDFGFVDILPSRDALNANLAALHEFFGQIRYAFKNP"
     gene            complement(8856..9605)
                     /locus_tag="PLD15_00900"
     CDS             complement(8856..9605)
                     /locus_tag="PLD15_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB62483.1"
                     /translation="MEKRPLLLGHRGMGTGDGENSLLSLVRAVQIGADGVELDVHPTR
                     DGVLVVTHDRDLKRTLGVDITVPESTYRLLKKKGLFYPEKLTTLEKVYLELPDTAFIN
                     IEIKDPLAAPLAVPLVRSFNALDRTLFSSFHHGCLLEIRKQCGDAKIGLLIGEDARGK
                     DPLAYFGELLSVYKPYSMNLPVQMFEEVGFKNGMEFIRFLRKTGIAVALWTLDDPDLF
                     LKLKGEIDIVITDNLQGMMALTGGRSSGKRG"
     gene            9800..10111
                     /locus_tag="PLD15_00905"
     CDS             9800..10111
                     /locus_tag="PLD15_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB62484.1"
                     /translation="MERIVEHPLLKFERGEKIVFYFEDVQMQAYEGETIAAALHANGV
                     RMLRITPKGSRPQGLFCAIGKCSSCLMEVDGKANVRTCITPVKEGMRVRTQKGRGEKS
                     E"
     gene            10104..11918
                     /locus_tag="PLD15_00910"
     CDS             10104..11918
                     /locus_tag="PLD15_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB62485.1"
                     /translation="MNNPDLLVLGGGPAGLCGAIEAAREGLRVLVVDEGISPGGQLVK
                     QTHKFFGNENFYASTRGMEIAGKLLKELSLYPQVILACESSVLAVYPDGVPVLDRRSD
                     STALYNPERLLVATGASERFLQFDNNDLPGVYGAGAVQTLMNQFGILPGKSILMIGSG
                     NIGLIVSYQLAQAGVEVKAILEASSRIGGYRVHADKVKRLGIPVLLNHTILKAIGEKS
                     VNGALITALDERWRPVPGTEREMVIDTVCIAVGLTPSAELVAQAGGQLKYIPELGGYV
                     PLHDSDMRTTIDRVFVAGDVSGIEEATTAMIEGRIAGLNIVKDIKGKCNETRLEELKE
                     TLSAFRSGPTSSKIRSGLEKMDLVTQPARYVEGEVNRSGEFRGKLRPIIECFEAIPCN
                     PCETSCPVGAITVGENINDVPVIDYSRCTGCGVCATKCPGLAIFMYQEREDGMALVGI
                     PYEFLPVPEKGQSVYATGRSGEVLCTARVDRVTRSANKTNIVYLTVPEELGRDVRHIH
                     VTNREESSLICRCEEITVEQVKKAIREGYVDFEELRRYLRVSMGPCGGRTCRLNTLMI
                     LSGETGVPVEKLSPGTLRPPAIPVSFGAIERNAKVTHE"
     gene            12035..13177
                     /locus_tag="PLD15_00915"
     CDS             12035..13177
                     /locus_tag="PLD15_00915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB62486.1"
                     /translation="MKNKACVTIIGGGVVGSATAYYLAKNGLTDIVLLERDYLSSGST
                     GRCGGGIRQQWSERMNVRLAMRSVEHFKNFEREVGLNIEYFQGGYLLLAYTEEEEALF
                     KKNVAMQQEEGLDVVLLSREETVRKFPFMNLEGLKLSAYCPSDGHANPHLTTFGYARA
                     AEKMGVNVLTHTDAKRVLVENGKIRTVVTDRGEIETGIVVNAAGGYSHQAGEMVGLNL
                     PTESYRHQIFVTEPLEHILDPLVISFVNNFYIRQTGSGNFIMGQGDRDELPGLEITPT
                     WKFLKEMSDKMPRFFPFLKDVRILRHWAGLYNMSPDAQPIIDRSNEIEDFYFAIGFSG
                     HGFMLAPAVGEALAEWIVFGEPRSVDISNLSLQRFERGFTREKNVV"
     gene            13198..14772
                     /gene="pruA"
                     /locus_tag="PLD15_00920"
     CDS             13198..14772
                     /gene="pruA"
                     /locus_tag="PLD15_00920"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPB62487.1"
                     /translation="MLEATNYMVIPPFRNESYIDPSDPGMREKMWRAIELLRSEHKEY
                     DLVIGGESISTEKKILSHNPANPEEIVGRVSKANREIVDKALKCAYKTFESWRLLPAF
                     ERIKPFLRAAEIMRYRRFELDATMILEVGKSWIEADADLAEAIDFLEFYSREALRYDE
                     PQPVVQVPGEYNLLRYIPVGVGAVIPPWNFPCAIMAGMTTAAAVSGNCVLLKPASDSP
                     VIAAKFVQILKEAGLPDGVVNFIPGSGGEIGDYIVENPAIRFVSFTGSKEVGLRINEL
                     AARHRAGQKWIKRVVLEMGGKDCVVVDETADLEAASIGAVASAFGFQGQKCSAGSRII
                     VVEDVYEEMVAKIKEKTEKLTVGETTDPDNHMGPVINEDAMKRILGYIDKGKSEGRLL
                     TGGKPLGGTGFFIEPTVFFDVPPDAVIAQEEIFGPVTAIIKAKDFEDAMRIANGTEYG
                     LTGSLYSKNRERIERAKAILHVGNLYFNRKCTGALVGVQPFGGFNMSGTDSKAGGRDY
                     LLLFLQGKSISERIIP"
     gene            15080..16258
                     /locus_tag="PLD15_00925"
     CDS             15080..16258
                     /locus_tag="PLD15_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB62488.1"
                     /translation="MSLLPEKIGAAFASVNPRLSKVFLPWIMKNPRYLRGAPKLLKAF
                     NEAEDIRVSLMAEEDVMIPPIMILSITNRCNLSCAGCFARTLGNSGTSTTGRSVMTVR
                     DWENVIDQGRELGVYTFLIAGGEPFMLPGLIDICLGNSDRIFVIFTNGTAIGEAELQK
                     LKKTTNTAVVVSVEGGRELTDERRGEGVFESAVGTMERLHRNGIVGGISVTITRRNHR
                     FWMEDENLDYFIDRGAKLGFFTEYIPTDEGGSASVSRSPMGDLNVTTGGESEAVLKSE
                     ERKSFREKILYYKANKSLFIIHSPGDEELFGGCVSSGKGFIHVNPYGDLTPCPVSDIA
                     THNMKKASLKEGLSSPLFVEIRENEGMLENGDGPCALFSHQKELEELRRKVGAYKTGL
                     "
     gene            16367..16555
                     /locus_tag="PLD15_00930"
                     /pseudo
     CDS             16367..16555
                     /locus_tag="PLD15_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
     gene            complement(16593..16751)
                     /locus_tag="PLD15_00935"
     CDS             complement(16593..16751)
                     /locus_tag="PLD15_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62489.1"
                     /translation="MMVNWISVSGTETASPSNVVTPAKLIDGDVPLAIMLSSLIRNTT
                     AVFPDVGA"
     gene            complement(16999..17751)
                     /locus_tag="PLD15_00940"
     CDS             complement(16999..17751)
                     /locus_tag="PLD15_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB62490.1"
                     /translation="MNVKEVANLTGISVRALHHYDRMGLLCPRRNRENDYREYSESDL
                     DRLQQILFFKECGFSLQRIRDLLNSPSFDREKAFELQRKYLLHEKRRIETMLGTLERT
                     IESLKGVNIMTQKEKFDGFDFSKNPYEEEARRLWGNETVERSNAFLESKSGEEREVMA
                     KTMDELFKELAAIRKEAPDSEVAQRAIDRMYRLFNESFGYHYTLDVFAGIGKMYVDDE
                     RFTRNIDKYGEGLSVFLSNAMRIYAERGESAQ"
     gene            complement(17859..18512)
                     /locus_tag="PLD15_00945"
     CDS             complement(17859..18512)
                     /locus_tag="PLD15_00945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB62491.1"
                     /translation="MIKGMIFDLFGTIVSNHRLFGPVCEKMANDGTADFEELENEFVR
                     LYRKHFKDYHKSAFQPEKFYYYLLIDEMIRKFDLSGDIESYCNYMYSSFGKLPAYPDA
                     KILKKLFREYTIAIITNADTSFVNEAIRNNRIPHHVLLTSEMARSYKPSCEIFDRALH
                     LMGLERDDVIFVGDSIRVDMMGAAGAGIKGVLIDRSGSYPDYAPRITSLEELQHIVL"
     gene            18649..19329
                     /locus_tag="PLD15_00950"
     CDS             18649..19329
                     /locus_tag="PLD15_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62492.1"
                     /translation="MSREVRLLWLHSNLSKDGLPSLDDFAKKFSISTRQAKRDIKYLR
                     TNLGAPLSYSRERSGYSYTKPYDIPSLFSGSLGMEIPSRSEIRQTLLKAIEEEKALRV
                     LLENGRAVYFHPACSDERQEIFAGPGEGDGLLFIQVGEIIEIRKSRRKYMKEPMLWKR
                     RLPVDAVVEKAVFEKAGELFTHYFFDLEDLIDFILNNRGIRLKGEASAVDRIRNAANT
                     LLEVVDSD"
     gene            19322..20605
                     /locus_tag="PLD15_00955"
     CDS             19322..20605
                     /locus_tag="PLD15_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HPB62493.1"
                     /translation="MIRSKIVYLFLFLLFAAFACAGNLRELVYGESLPETLAEAGARV
                     VDTPSAGLIRIETLLFNPFNWQGIQWQNHLLIIRPPSVQSRVALIFITGDYSYSEDEL
                     VVFRLMALQNRAYVAVLFDVPNQPLFGGKREDWLISHTFLEFLRTGDPRWPALVPMVE
                     SAVVSMNLIHDYASSSGDTVEGFVLSGGSKRGWTTWLTAAIDERVEGIVPIAYDNLNL
                     ADQMRHQLDFWGSYSRSIREYVDSGILNDLDDPKKRELLELVDPFTYRDSLDLPKLIV
                     VGTNDPYWPVDAANLYIDDLPGYSAMVYAPNAGHGTEVYRVTQAIQGMISHLNTGLEF
                     PSIEIFLEETASNVKISLNVDERDSKLVELRLFHSLSAVRDFRKAFFEYETIGKEEEI
                     IVDRVSFNAFYIEGVFTFNGKELLISTPVKVIEAP"
     gene            complement(20639..>21151)
                     /locus_tag="PLD15_00960"
     CDS             complement(20639..>21151)
                     /locus_tag="PLD15_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62494.1"
                     /translation="TSIAMGKILYVMMIAVLNSVFTFIGLIIAFRLGGPAFGAGEMNF
                     SSLSPSTLAGLFVTLLTMAGLAASIIVLLGSLARNIKEGGGYILPIYIFAVVLGVATM
                     QMESPDNPLLYIIPLINSIFVMKDIITLQFVSWRFLLMLLSNLTYVSVLIFALAKVFN
                     SEKIMDSSGS"
BASE COUNT         5416 a         5245 c         5615 g         4875 t
ORIGIN      
        1 ctatcatcag acggcgacgg ccatatttac caatgggttg agcctcatcg ccggtttctc
       61 ggttctcttg ctgacccctc tgctcctgta cgtggatgtg gctaaactga tggtgagcgg
      121 actggcggtg gggatgatat tcactctggt tctccttccg gagatagcgg cgaggcgatg
      181 gaagcaaaat aaccggcata gcagcggata attcagaccc gaaggccggg attggagaat
      241 tcaatgaagg cgctggtaac tcttttgccc gatccaataa gcgaaatggt tcacctgata
      301 tggaacgagc tggagagaga cttcaatttc cgctccgtgc tggtcactcc atatccccac
      361 ttcacatact gcgtcgtcga agatttcacg gaagggctat acgaaaaaat cgaagcgacg
      421 atcacaaacg tcgattcctt caccgtcagg accgagtacc tgggcgtctt ttccggagag
      481 agcccggtgg tctatatagg gatcgccaga attccagaat tatcacactt tcacgatacg
      541 ctgtggagaa ggtttttcgg tccggaaaga gatatccacg acctgtacgg cattgctcac
      601 tggatacccc acataactct gatcttcggt agagacctta accgtgaaaa cttctgcccg
      661 gcgatggaga gactcgtctt ccgcgatttt caccgcgaat tcgaagtcaa caatctggct
      721 attctgagcg gctcgctaga gagtgtcata gaggtcgaaa agatattcta ttttccggga
      781 aaccaacatc cctaaatccg ataactatcc aatcgtcggc catgttaacc tatgcagtca
      841 tttgttatca ttggaaatag ggaggaggta tctcaatgcg aaaattgaac tggaaggaaa
      901 tcgatctcaa agtcacttta cccagaaagg tgaagtccgc taaatccata gacggactgg
      961 aagagttcat aggacaggaa cgcgctataa aggccctgga aacgggactt aaaataaagg
     1021 ccaggggata caacatcttc gtatcgggat cgaccaacac cggaagaagg acctttgtga
     1081 gccgttacct gaagaaaaag gccgagggcg gcgaaacgcc cggcgactgg atctatgtct
     1141 ataacttcga cgatcccaga tcacccaact cgatccccct gaaggccgga acgggaaaga
     1201 gctttcagaa agagatgggc gaattcgtcg aaataactat aaacgcgata agcgagagtt
     1261 ttcagagcga ggactaccag cagaaaatgt cggccataca gagcgagcaa gcggaaaacc
     1321 ggtcgaattt gctgaaagaa ctcatagaca aagccagaga gagggatttc acggtgcaga
     1381 taaatcagac cggggtggcc acgatccccc tctggaacgg aaagcccttg acgcaggaga
     1441 tatacgaggc tctgccggaa gagtaccaga agacgatcag caaaaagggc gaagaagtta
     1501 gagagctggt taactcctac atcctgagaa ttagcaaaat ggagaaggaa tacggagaga
     1561 agttcaaaga gctgaaccgg gatgtggcgt ctttcgcgtt ggaagggcac atcaaggaga
     1621 tgaaagataa attcagcgag aacaaagagg ccatagagtt cctcgataac ctgcggagcg
     1681 atcttctgga caatctcggc gttttcttca gccaggagat agacgccaag aatttcttcg
     1741 gaaagagata tgccataaac ctcttcgttg acaactccgg cataaatggc aagcctatcg
     1801 tcgaggtgac gaacgcgaat tactccagtc tcttcggaag gatagagtac ctggccaaga
     1861 tggggatgct tgacactgac cactcgatga tccgttccgg agcgatacat cgttccaacg
     1921 gaggcttcct tgtgctagat gccaagagtg ttttgagcga accgtacgtc tggcagacgc
     1981 tgaagcagct tctcttctcc ggttcggaag tgatcgagaa cctggaacac aaagtcggat
     2041 tgctctcgac ggttagcctg aagcccgaac cgattcctct ggacatgaag atcataatga
     2101 taggagagcc ctggatctac agcatgttga gcaccatgga cacggatttc aagaagctgt
     2161 tcaagataaa ggccgaattc gactgggaga tgcgactcga cggtgacacg gtcgggaaac
     2221 tctgtcggtt gatctgttct atcgcacgcg ataattcgct catggatttc gacagggatg
     2281 cggtcaggga agttataaag aaagcgataa tactatcggg aaacaggaaa aaggtttcaa
     2341 cccagttcgg agccctgaaa cagcttctac tcgaaagttc ggccgttgtg gagattaacg
     2401 gcggagagat ggtctcggct gaagacgttt ctcgggcctg ggaagagatg aagacaagag
     2461 tttctctgta ccaggataag gtgaaggaga tgttcgccga ctccacgctt ctcgttcaaa
     2521 ccgaaggcga gctgatcgga gagataaacg gtttgacggt cgtgcagacc gaagatctat
     2581 cgttcggaat accagtcaaa ataaccgcca aggccggtcc tggaaacagc ggcatagtgg
     2641 atattcagaa ggaatcggaa ctgagcggca atatccacga caaggccggc ctcactattc
     2701 aggggtacat gaactcgaaa tatgcgcggg aatttcccct cagcctgaac ggctcgatca
     2761 gcttcgagca ggtatactcg atggtcgagg gcgacagtgc ttccgtggcc gaaaccgtcg
     2821 ccttcctctc ggcgattgct gaagctccca taaaacaatc tatagccgtt acaggctcga
     2881 taaatcagag cggaagagtt cagccagtcg gaggcgttca gttcaaagtg gaaggttttt
     2941 acgaactctg caaattgaag ggtctcaacg gaaagcaggg cgtggtgata cccgacacaa
     3001 acttcgacaa tctcgttttg cccgacgaga tcgttctggc cataaaaaac ggcaggttca
     3061 acatctggac tgttgaaacc gttgatgagg ctatcgaact tttgaccggc ctgaaagcgg
     3121 gccagcccga cggagatggt aaataccccg aaggcacgtt caacaacctg gttcttcaga
     3181 ggctggagag attctacgaa atctcgatgc aggagaagaa ataacgacag ggctaggaac
     3241 gagaagagca ttaggagccg tccagcccct agtccaagag cgtgaacccg ttcttcgaaa
     3301 gagcggttcg ccgttccgac gctgtgcgtc caggttcttg gttggttaag gaacgcgaga
     3361 agaggagaga gaaagagaga ggagagaggg cgagacacct tctcgtgagt gaagcgaact
     3421 ctcgaatgca ctctttctcg tgatttttgc aataacctac cacataattt tgtagacagt
     3481 actatccccc tattttaatt ataggtcata ttaactcccg atgtgttact ggccttaatt
     3541 caaggctctc agcaatgata gagtttattt agtttgtgat ccgaatcgaa tctgttcgcc
     3601 tgttttcaaa agttcattac ccattattct taatactatt aatggccggt ttttaccgac
     3661 cagatttttt gtgtttttct ggaggtgtaa aatgtcaagt agttccagat ttccaaggag
     3721 cagtattgga aagtctaatc cgctcttttc tcagaccagg actaccgtcg agactgcctt
     3781 ttacggcaac aacgtttcga cggtgatttc accctgcgaa gcttacaaac tggcgaagag
     3841 ttcgcccggc acgatcgtga ccgacatgcc cgtctataaa ccggaaaaac tcggtctcga
     3901 acccgactcg aaggtactta tattcaacga tggcgccgtt acaggtcgct gcgcttctgc
     3961 aagacggata ctcggtgaac cgggagtgag cgaggcggag tatggtgcga aaataagaga
     4021 ggctgtgtac agcaccagaa agaagaagat gtaccatgcg cagggttata tcggtctcga
     4081 ccgtgatttc atgatcaaag cacatctcct cattcccgag aaccacgaaa atatcatgta
     4141 caactggctt ttgaatttcc agccgatgaa cgagttctat aacgagatgt acgagaattc
     4201 gaaaaagtac gagggtgagg gcgagatatt cgtcttctcc gatcccgact ggcagcatcc
     4261 ggagcatccc ctcgggctca gctttttcga tcccgaacac aactgcgccg cgatactcgg
     4321 tatgcgctat ttcggcgaat tcaagaaagg gactctgaca ctggcctggg gttgcgccaa
     4381 cagacacggc ttcgcggcct gtcacggagg tcaaaagagg tacaatttac ccgatagaaa
     4441 atacgttgca ggcttcttcg ggctctccgg atcgggcaaa tcgacactga cccatgccaa
     4501 gcacgggggg aagtacgatg taaccattct tcatgacgac gcgttcgtta tctcctcgga
     4561 aaacggctcc tcggtagcac tcgagccttc gtatttcgac aagacctcag attatcccct
     4621 ttgcagtgat gacaacaagt accttctctc cgttcaaaac gtcggggcaa cgcaggacga
     4681 aaacggtaat atagtgattg tttccgagga tatacgcaac ggaaatggca gggccataaa
     4741 atccaggttg tggtccccca acagggtcga tagattcgat gaaccggtcg atactatcgt
     4801 atggctcatg aaggatccgt cgataccacc cgtggtaaaa atcgaggatc ccgtactttc
     4861 ttcggcaatg ggggccacgt tggccaccaa aaggacctcg gccgagaggc tcgctcccgg
     4921 ggtagatccc aacgctctgg ttatagagcc ttacgccaac ccattcagaa cataccctct
     4981 atcggatgat ttcaaaaggt tcctcgagtt attccagaag agaaacatcg attgctatat
     5041 attcaacacc ggacatttca gggagaggaa gattcccaaa gagttgaccc tcaatatact
     5101 ggagaatata gtcgagggca aggcccggtt caagaaatgg gaacctttcg aagagctgga
     5161 gataatggaa ctggaaggct tcactccgga tgccaacgac ggagattaca tgaaacttct
     5221 tagagacaga ctggttgaca gattcaactt cataaagagc cgtgaaagcg aaaggggtgg
     5281 cttcgataga ttgccccccg aggccattga agtcttcgca aagctcatcg aaaggctcag
     5341 atagggacag cacatagcaa taaaggccgc cttcgggcgg cctttttgtt atcctttgct
     5401 aatgatctga ccttggttga caattgtatc taatgtagaa tattgtttgt acggtaaaaa
     5461 tccactcttt cgaagcgggc gagtggcgcg caacaaaatc tattagattg aagaaggttc
     5521 tatatgaccg ttcccgtagt tcttgaagag tttataaata gattcaaagg ctggatttcc
     5581 ggttccggcg atctgacgga gctggtggga gctttctctt cgctctcgac ttacacgatc
     5641 atagctttcg tggcgatagg cctttcgctt ctgatattcg gcaagaaact cttcgcggtt
     5701 ttctacgccg tcctctggac agtcatagga tctcatgtcg gttatctctg gggacaggga
     5761 atggagacgc tgtacacgat cgagcttatg atccttatcg gggtccttct accgctggtg
     5821 gcgatcaagg ctagagaaat attcgtcttt acgatggttc tcttcggaac ggggtttttc
     5881 gcaacgtata tcgcccagag cctcggtacc tttcagagtc tgacgattct cgatccgccg
     5941 ttcgtcctgg cgggctgtct tctcggctgg ctggcggtga ggttcatgga ttacgtgatg
     6001 atagtatcca cctcgctgat cggagccttc ctgttgatgc tcgcggtgag ttattttttc
     6061 agagatatca ccgacctttt gaccatgctc cttgtctttc ttgcaacgtc tctgttcggg
     6121 atattccttc aaatggacgt atatctgaaa aacaaaaaga gcagtaagaa aaagaaaaac
     6181 agatcgaaag gagcaaagag atgataagaa taatcctttc cgaccggatg gaagagaact
     6241 gctatctggt cgaagacgaa aacgggatag tcgttatcga tccgggaata ggagcagccg
     6301 accggataat cgatcttgtg ggagatacaa aactctcgat ccttataact catggacatg
     6361 ccgatcacat cttcgatatc gacagactga aaggcgataa catcttcgtc catccgctcg
     6421 acagagagag actcctcgac gctgaaaaga gccttctgaa tttctttgga aggggtcccc
     6481 ttaagataga cgaatcgact atcgtggatt gctcgcaaat cggcggaaag tggagagtgc
     6541 tacacactcc cggacacacc cccggctcgt gctgttatct gtacgacgac agaatacttt
     6601 tcaccggaga tacagtcttc atgcagtccg taggcagaac cgatcttcct ggcggctccg
     6661 aagagacgat gatcggcagc ctacacctgc ttaaagagat cttcgagaac ggaccggatc
     6721 tgaaggtctt tcccggtcac ggtcccggga cagacgcccg tgaaattctg accgacaatc
     6781 ctttcttcag atagatccgg gcctcttgaa gttcttggtc ttcaattcct gaagctctct
     6841 ctctttcttc ttccagtatc tcctgttgaa aagaccggtt tctacgggac tctcctggag
     6901 cagagaagcc atttttttcg cgagtttcca gcccgtgtcg aaggcctggg cggtgatctc
     6961 gcggtgctcc agggagtctt tgtgcaggat caactcctct tcgaacggca aacagtactg
     7021 ctcccacaac tgtctggctt tctcatagcg ggtcgtcgcc agacgatgga agatctccca
     7081 gttgccccag tgattgagtg cgtcggcctc cttctcgaag ggcagggtcg aggaattgga
     7141 gtcgaaccag acgggtttgt agaaagaaag gcaaggcgcg ctggtattgg ttacccagac
     7201 atcgattttc tcaccgagaa ccacgaccat agagccggtg gtctgtgaat tcaccaatcc
     7261 ggtaccggag tgcatacaga cgttcttcat ggaaccgaaa aacctcgatc cgccgtgatc
     7321 cctgagcatt tcgaaggccc ttttcagatc tacatctttc tctcccagaa gattcatcgt
     7381 tcggctccgg cgagcctctc caccggagag ccatgaaaag agcttcttct cgtagcattt
     7441 cttgaaacgg aactcttcgg cctttttaca ccagcccttt tccagtgcgt tgctcgcggt
     7501 ttccggatgc tgcaattccc cgggacctgc gatcgtcaga cagttcgaga tcgacgctcg
     7561 atccttcact cgatcggcgg tccagtatct gtcggaagtt tccagaaccc acgcgttttc
     7621 cctgtcggcc actatgtagg agttgtggta gaagatcgtc ttcgtgtaac ccgcactctc
     7681 gccctggccg tacttctgta tgagatcggt gataacggtt acggcctgtt cggcgctttc
     7741 cgatctttcc agggcaatcc tgagaatatc catccccagc aacccctgcc tgctgaccat
     7801 ctctctggtg aagactgctt cgttgccgat cgctacgccg ttttcgttca cccccatctc
     7861 gccgccccac atccatgagg gcttggagat gagcaccgcg taggttttct cgacctgttt
     7921 tatcttctgc gaggtggtgt agagctcctt gccgctgtat tccgtccggg gatagaagaa
     7981 ggcgtattga ggttcgttcg gctctctatc gctgtttttg gcgaaaatcg ttctgccgtc
     8041 aggcgtggag cttcccagag ccacaaaggt atcacacatc taactctcct ttcaagggtt
     8101 cttgaaagcg tacctgatct gaccgaaaaa ctcgtggagc gccgcaagat ttgcgttcaa
     8161 agcgtctctc gaaggaagaa tatccacaaa tccaaagtcc gtatggtcat acaggtaatc
     8221 ggttgggtat ggcgtaacga tcaggccgaa ttcctcgaag gacttcacgg ccctcttcat
     8281 atgtacggcc gaagtgacga ggaacacatt cttccagccg ctttgtgagc atagtctggc
     8341 ggtgtattcc gcgttctccc aggtgtttct cgcctgcggt tccaccagaa tcctggaggt
     8401 gccaactccc tgtccgagca accagtccct catcagttcg gccaccggca gagtgtttct
     8461 tccgggtacg aaagatccgg ttactactat gggcagttta gtctgaagat ggaggttcaa
     8521 agccgcactc agcctggcca cactgtgatt gtccagcacc tgaccgaccg gagttatgat
     8581 cactccgctg ctgagaacaa ctataacctg gccgctcacc gattttggta actgatactc
     8641 tttttctagc ggccgcagat agaggtacgt tcccagccct gttgaaagaa gatatccaaa
     8701 gaaagagaga atcagcagaa ctattccacc ggctcttatc ccgcgagcat cctttttcct
     8761 cagaagagta agcgctaaaa tcagcagaat taggataaca aggccgggcg gtgtcagtat
     8821 agaagaaagt atcttctcga gataaaacaa tctcatcacc cccgctttcc tgagctcctt
     8881 cctccggtaa gtgccatcat gccctgcaag ttgtcggtta tcacaatatc gatttcgccc
     8941 ttcaacttga gaaataaatc aggatcgtcc agagtccata gagcgacggc aattcccgtc
     9001 tttcgaagga atcttatgaa ttccataccg tttttaaaac ccacttcctc gaacatctga
     9061 accggaagat tcatcgaata aggcttatag acggacaaca actccccgaa ataagccaga
     9121 ggatctttac ccctggcatc ttccccgatc agcagaccga tcttcgcatc gccgcactgt
     9181 ttgcggattt ccagcaagca accgtggtgg aacgatgaaa agagcgttct atcgagagcg
     9241 ttgaagctcc gtaccagcgg aaccgccaga ggagcggcca gaggatcctt tatctcgata
     9301 ttgataaagg ccgtgtccgg aagctccaga tagaccttct cgagagtcgt caacttctcc
     9361 gggtaaaaca gacctttctt tttcaataat ctgtaagtac tttccggaac tgtaatatct
     9421 acaccgaggg ttctcttgag atcgcggtcg tgcgtcacca ccaatacccc gtctctggtc
     9481 gggtgaacat cgagctctac gccgtcggcg cctatctgaa ctgccctcac cagcgagaga
     9541 agagagttct ccccgtctcc tgtgcccatt cccctgtgtc ccagcaagag tggtctcttt
     9601 tccatgtatt ctttgccccc cagtgatcgg gagtagagat tgcgttcgtt ttcaactgca
     9661 tccctattcc ggcaaatctt tatacagaca attatagcag cttattttgt ttgttcggga
     9721 gtatgcatcg gtatgctggc cttcttcctc agagccggaa tacttataat tgtaatgctc
     9781 agtaaatgga ggcgatatct tggaaagaat cgtagaacat cctcttctca aattcgaaag
     9841 aggtgaaaag atcgtttttt attttgaaga cgtgcaaatg caggcatacg aaggcgagac
     9901 aatagcggcc gcccttcacg caaacggcgt gagaatgttg cgaattacgc ccaaaggttc
     9961 aagacctcag ggactgttct gcgcaatcgg taagtgttct tcatgcctga tggaagtcga
    10021 tggaaaagcg aacgtaagaa cttgcatcac tccggtgaaa gagggtatga gagttcgaac
    10081 gcagaaaggc agaggtgaga aaagtgaata atcccgatct tctagtcctg ggcggagggc
    10141 cggccgggct ttgcggcgcc atagaagcgg ctcgtgaagg gttgagagtg ctggtggtgg
    10201 acgaaggtat ttcgccaggg ggacagctgg tgaaacagac ccataaattc ttcggaaacg
    10261 agaacttcta cgcttccacc agaggaatgg aaatagccgg caaactgttg aaggaacttt
    10321 ctctttaccc gcaggtcatt ttagcttgcg agtcatcggt ccttgccgtc tatcccgacg
    10381 gcgttcccgt tcttgacagg cgaagcgaca gtaccgctct atacaatcct gaacgactcc
    10441 ttgtggctac gggcgcttcg gagagatttc tgcagttcga caacaacgat ctccccggag
    10501 tttacggggc gggtgccgta cagaccctga tgaatcagtt cggaatcctt cccggaaaga
    10561 gcatactcat gatcggttcg ggcaacatcg gattgatcgt ttcttaccag ctggcccagg
    10621 ccggagtcga agtcaaagcg atactggagg cttccagccg cataggcggt taccgtgtac
    10681 acgcggacaa ggtaaaaaga ctgggcatcc ccgttctcct gaaccatacc atcctcaagg
    10741 ctatcgggga gaagtccgtg aacggcgcgc tgatcacggc cctcgacgag cgctggagac
    10801 cggtgccggg aacggagaga gaaatggtga tagacaccgt ctgtatagcc gtagggctca
    10861 caccatcggc cgagctggtg gctcaggcag gaggccagct gaaatacata ccagaactcg
    10921 gcggttacgt tcccctgcac gatagcgata tgagaacgac gatcgacaga gttttcgtgg
    10981 ccggggatgt ctctggtatc gaggaagcga ctaccgcgat gatcgaaggc agaatcgccg
    11041 gtcttaacat agtgaaggat ataaagggga agtgtaacga aacgcgactg gaagaactca
    11101 aagaaaccct gagcgcgttc cggagcggac cgacatcttc gaagatacgc tccggtcttg
    11161 agaagatgga tttggtgaca caaccggcca ggtacgtaga gggcgaggtg aacaggtccg
    11221 gggagttcag gggaaagttg agaccgataa tcgaatgttt cgaggcgata ccgtgcaacc
    11281 cctgcgagac gagctgcccg gtcggagcga taacggttgg agagaatata aacgacgtgc
    11341 ccgtcataga ttattccagg tgtacgggct gtggagtctg cgctaccaag tgcccgggtc
    11401 tcgcaatatt catgtatcag gagagggagg acggaatggc tctggtggga atcccctacg
    11461 agtttctccc cgttcccgag aagggccagt cggtttatgc aaccggcagg agtggagagg
    11521 tactctgcac ggcaagggta gatcgcgtca ccaggtcggc aaacaaaacg aacatagtgt
    11581 atcttaccgt tccagaagaa ctgggtagag acgttcgcca catacatgtg acgaacagag
    11641 aagaaagctc tctgatctgt cgctgcgaag agataaccgt cgagcaggtg aagaaagcca
    11701 taagagaggg atatgtcgat ttcgaagagc tgcgaaggta tctgagagtg agcatgggac
    11761 cgtgcggcgg cagaacctgt cgtctcaaca cgctcatgat tctttccggc gagacgggag
    11821 tgcctgtaga aaaattatct ccgggaaccc tgaggccgcc ggcgattccg gtgagtttcg
    11881 gagcgatcga aagaaatgcg aaggtcaccc acgagtgaag tagcaggctt ggcccgaagt
    11941 tgactagcct gagtgtttcg gacactacgt tatatgaaag gagggaatgt cgctttccgc
    12001 gaattagttc acgggtctct aaagtgacta ctttatgaaa aataaggcgt gcgtaacgat
    12061 aatcggtggc ggtgtcgtcg gttccgccac ggcctattat ctggccaaaa acggacttac
    12121 cgatatagtg cttctcgaaa gagattatct ctcttccggc tcgaccggaa ggtgcggtgg
    12181 gggaatcagg cagcagtggt cggaaagaat gaacgtccgg ctggccatga gaagcgtcga
    12241 acacttcaaa aacttcgaaa gggaagtcgg actgaatatc gaatactttc agggagggta
    12301 cctgcttcta gcgtacaccg aagaggagga ggcactcttt aagaagaacg tcgccatgca
    12361 acaggaagag ggactcgatg tcgtcctgct ctcacgggaa gagacggtcc ggaaattccc
    12421 cttcatgaac cttgaaggtc tgaaactgtc ggcgtactgt cctagcgacg gccatgccaa
    12481 tccccacctg actaccttcg gctatgcgag ggccgctgag aaaatgggag tcaacgtact
    12541 aacgcataca gacgcaaaga gagtgctggt agagaacggc aaaatcagga ccgtggttac
    12601 tgacagggga gagatcgaaa cgggaattgt ggtcaacgcc gcaggcggtt attcccacca
    12661 ggcgggagaa atggtcggac taaaccttcc aaccgaatcc tacagacacc agatattcgt
    12721 taccgaaccg ctggagcaca ttctagaccc actggtcata agcttcgtga acaatttcta
    12781 tataaggcaa accggatcgg gaaatttcat catgggacag ggcgacagag acgagcttcc
    12841 gggactggag ataacaccta cctggaagtt cctcaaggag atgtctgaca aaatgccacg
    12901 gttctttccg ttcctgaagg atgtgaggat cctcaggcac tgggccggac tctacaacat
    12961 gtctcccgat gcgcaaccga taatagacag gtcgaacgaa atagaggact tctactttgc
    13021 aataggcttc tcgggacacg gtttcatgct cgcaccggca gtcggagagg ccctggccga
    13081 atggatagtc ttcggtgagc ccaggagcgt ggatatatcc aacctgagcc tgcagagatt
    13141 cgagcgtggt ttcaccagag agaaaaacgt agtataggaa atcataggag gtaagaaatg
    13201 ctggaggcaa ccaattacat ggtaataccg ccattccgta acgagtctta catcgacccc
    13261 tccgacccag gtatgaggga gaaaatgtgg agggctatag agttgctgag atcggaacac
    13321 aaggagtacg atctcgtgat cgggggagag tccatctcaa ccgaaaagaa gattctctcg
    13381 cacaacccgg caaatcccga agagatcgtc ggtagagtct cgaaggcgaa cagggagata
    13441 gtggataagg ccctgaagtg cgcttacaaa accttcgaaa gctggagatt gttgccagct
    13501 ttcgaaagga taaaaccttt cctgagggcg gcggaaatca tgcgctatag acgcttcgaa
    13561 ctcgatgcca cgatgatact ggaagttggg aagagctgga tagaggccga cgccgatctc
    13621 gccgaggcga tagatttcct cgaattctac agccgtgagg cgctgcgtta cgacgaacct
    13681 caaccggtcg ttcaggttcc gggagagtac aatctattgc gatacatacc agttggcgtc
    13741 ggagcggtga ttccgccctg gaactttccc tgcgccataa tggccggcat gacgacggcc
    13801 gcggccgttt ccgggaactg cgtcctgctc aagccggcct cggattcgcc ggtgatcgcc
    13861 gcaaaattcg tacagatact gaaggaagcc ggtctccccg atggagtcgt aaacttcatt
    13921 cccggctccg gcggagagat aggcgattac atagttgaaa atccggccat ccgtttcgta
    13981 tccttcaccg gatctaagga ggtcggcctg aggataaacg aactggccgc caggcatcgt
    14041 gccggtcaga aatggataaa gagagtcgtt ctggaaatgg gcggtaaaga ctgcgtcgtc
    14101 gttgacgaaa ctgcagatct cgaggccgct tcgattggcg ccgtggcgag cgctttcggg
    14161 ttccaggggc aaaaatgctc ggccggctcg aggataatcg tggtcgaaga cgtttacgaa
    14221 gagatggtgg cgaagatcaa agaaaagacc gagaaattga ctgtcggaga aaccaccgat
    14281 cccgacaacc acatgggtcc ggtaataaac gaggacgcca tgaagagaat actcggctat
    14341 atcgataaag gaaagagcga gggaagactc ctgacaggag gcaagcctct cggcggaacg
    14401 ggcttcttca tagagcctac ggtttttttc gacgtgccac cggacgctgt gattgcgcag
    14461 gaggagatct tcggtcccgt aacggccatt ataaaggcga aagatttcga ggatgcgatg
    14521 agaatcgcca acggaacgga atacggactt accggctctc tctacagcaa gaatagagag
    14581 cgtatagaga gggcgaaagc cattctccac gtgggtaacc tctacttcaa caggaaatgt
    14641 accggtgccc tggtgggagt gcaacccttc ggaggattca acatgtccgg taccgactcc
    14701 aaggccggcg gtagagatta tcttctgctc ttcctccagg gaaaatcgat aagcgagagg
    14761 ataatcccct gataatcgag agttcgctcc gctcacgaga aggtgaaaaa caagagagaa
    14821 agagcatcga gagttcgctt cgctcacgag aaggcgagaa ttctctctct ttctctctct
    14881 ttctctctct ccctctctcc tcttctcgcg ctccttaacc aacgaagaac agcttctacc
    14941 gctcctacca gacccaagac tcagaacgca cgtgaatacg agatcgataa atcgatttca
    15001 gacaaactat aaagctgagt aacaaaagat taatatttca ggaataaact tcatttcgtt
    15061 aggggggatc aaattgaata tgagtttatt accggaaaag atcggggcag cttttgcgag
    15121 cgtcaatcca agactttcga aagtattcct tccatggatc atgaaaaatc ccagatatct
    15181 gagaggagcg ccgaaactcc ttaaggcctt caacgaagcc gaagatatcc gtgtctctct
    15241 aatggcggaa gaagatgtaa tgattccgcc gataatgatt ctcagcatca ccaaccgctg
    15301 caatctcagc tgcgccggtt gttttgcccg caccctgggc aactcgggaa catctactac
    15361 gggcagatcg gtcatgacgg ttcgagactg ggaaaacgtt atagatcagg gaagagaact
    15421 gggagtttat acttttctca tcgcaggcgg agagcctttc atgttaccgg gcctgataga
    15481 tatctgcctc ggcaacagcg acaggatctt cgttatcttc accaacggca cggctatcgg
    15541 ggaggccgaa ctccagaaac tcaagaaaac gaccaacacg gccgtggtcg taagtgtcga
    15601 gggcggaaga gaattgaccg acgagagaag gggcgaggga gttttcgaga gcgccgtcgg
    15661 tacaatggaa agacttcaca gaaacggaat cgtcggaggg atatcggtta cgataacaag
    15721 aaggaatcac cgtttctgga tggaagacga aaacctggat tacttcatag accgcggggc
    15781 gaaactggga ttcttcaccg aatacatacc gaccgacgag ggaggttcgg cgtctgtttc
    15841 gcgtagcccc atgggggatt tgaacgtaac caccggtgga gaaagcgagg ccgtactgaa
    15901 gagcgaggaa agaaaaagct tcagggagaa gattctctat tacaaagcca ataaatcact
    15961 tttcataatc cactctccgg gcgatgaaga actgttcgga ggttgtgtat cgtctggaaa
    16021 aggcttcata cacgtcaatc cctacggcga tctgacaccg tgtcccgttt cggatatagc
    16081 gactcacaac atgaagaaag cctccttaaa ggaaggcctt tcgagccctc tcttcgtcga
    16141 gattcgcgaa aacgaaggta tgcttgaaaa cggcgatggt ccctgcgcgc tcttctcgca
    16201 ccagaaagag ctggaagagc tccgcaggaa agtcggggcg tacaaaaccg ggctgtaaga
    16261 gcgttaagag agagagaaag agagggagag agagggagag gcgccttctc gtgagcgaag
    16321 cgaactctcg acgctccttc tctcgtgttt ttcgctctcg ccggagggac tgtgcagcga
    16381 aatgcacctg acgatcgaag ggggcgctat cataatcaac tcgcaggacg acggaatcga
    16441 cgccgacatg gatattacga tcaacggggg cacggtggtg gccctgggca gtcgaaacga
    16501 cgtaaccagc agagagactt cggcgcgttc ggtagctcat ctccggaatt catagtaacc
    16561 gccaaccagc acagtttcgg caatgtcaac gactacgcgc cgacatcggg aaaaacggcc
    16621 gtggtgttcc gtatcaacga cgataacatg atagcgagcg gcacatctcc atcaataagc
    16681 tttgcgggag tcaccacgtt cgagggagaa gccgtttccg tacccgagac ggatatccag
    16741 ttcaccatca ccgacgtacc ttcggaaaat tactcggcct cgtgccttct gagcgatgga
    16801 gaggctgcgc ttaaagcgat aatacccgcg gatgcgggca gttgttctca ctatcgcaat
    16861 cgtcgattcc aactcacaat acaccggaag cggtcaatgg gcattcacga tcaagtgaaa
    16921 attgtgaacc tttccactct gtagtaaggt atcgacattt atcggattaa aaagaagaga
    16981 gccccggggc tctcttcttc attgtgccga ttctccacgc tcggcgtata tgcgcatcgc
    17041 gttcgagaga aagacagaca acccttcccc gtatttgtcg atgtttctgg tgaagcgctc
    17101 gtcgtcgaca tacatctttc ctattcccgc gaagacgtcg agcgtgtaat gataaccgaa
    17161 gctctcgttg aagagtctgt acattctgtc gatagccctc tgcgccactt cggaatcggg
    17221 agcttctttg cggatcgcgg ccagttcttt gaaaagctca tccatagttt tcgccatgac
    17281 ttccctctct tccccggact tcgattcgag aaaggcgttg cttctctcca cagtctcgtt
    17341 tccccagagc cggcgcgcct cttcttcgta cggattcttt gaaaagtcga acccgtcgaa
    17401 cttttccttc tgtgtcatga tattcactcc tttcagagat tcgatggttc tttcgagtgt
    17461 gcccagcata gtttcgatac gcctcttctc gtgcaacagg tactttctct gcagctcgaa
    17521 ggccttctcc ctgtcgaaag acgggctgtt caaaaggtcg cgtatccttt gcagggagaa
    17581 gccgcactcc ttgaagaaaa gtatctgttg cagcctgtcc agatcgcttt ccgagtattc
    17641 tcggtagtcg ttctcccggt tgcgccgcgg gcacaggagc cccatcctgt cgtagtggtg
    17701 aagcgcccgt acgctgatac cggtaaggtt cgccacttct tttacgttca tcggcctccc
    17761 ctcctcgcag ttccaatcat aaactatgac gcaaggtgat agtcaagacc gctaagagga
    17821 atggttttct ttgtcgcgac tcgaagcgaa aagcgatctc aaaggactat gtgctggagt
    17881 tcttcaaggc ttgtgatcct gggagcgtag tcgggataag atcccgaacg atctatcagc
    17941 actcccttta taccggctcc ggccgccccc atcatatcca ccctgatgct gtctccgacg
    18001 aatatcacgt catctctctc caggcccatc aaatgaagcg cccggtcgaa tatctcgcaa
    18061 gagggtttat aagatctcgc catttccgag gtcaatagga cgtggtgggg gattctgttg
    18121 tttcttatcg cttcgttcac gaaggaagtg tcggcgttgg tgattatcgc gattgtgtat
    18181 tctctgaaga gtttcttcag gatcttcgcg tcgggatagg ccgggagctt gccgaaggaa
    18241 gagtacatgt agttgcagta agactcgatg tctccgctca ggtcgaattt ccttatcatc
    18301 tcgtctatta gcagatagta atagaacttc tccggttgaa aggccgactt gtgatagtct
    18361 ttgaagtgct ttctgtatag cctgacaaac tcgttttcca gctcttcaaa atcggccgtg
    18421 ccatcgtttg ccatcttctc gcagacgggt ccgaagagtc tgtgattgct aactatggtg
    18481 ccaaagagat cgaaaatcat acctttaatc atcagagact ccttttatgt atgaatattg
    18541 tggacatatt agggcgtttt ttgtataatt gaatagatat attttagcat ctgttccggc
    18601 ttacgatcta cttgtgtttc ggcgctgagg ggacaggagg tgtttcttat gtcccgtgaa
    18661 gtacgtcttc tatggcttca cagcaatctt tcgaaagacg ggttgccttc gctagacgat
    18721 tttgcgaaaa agttcagtat atccaccagg caggccaaga gggatatcaa gtacctgagg
    18781 accaatctcg gggctcctct ctcttattcc agagagaggt ccggttattc atatacgaag
    18841 ccttacgata ttccctcact cttctccggt tcgctaggta tggaaatccc tagcagaagc
    18901 gagatacgac agactctgct gaaggcgatt gaggaggaga aggctctcag agtcctgctg
    18961 gaaaacggaa gggccgttta cttccatcct gcctgttcgg acgaaaggca ggagatattc
    19021 gccggtccgg gagagggaga cgggcttctc ttcatacagg tcggcgaaat aatagagatc
    19081 cgcaaatcca gaagaaagta tatgaaagag cctatgctct ggaagaggag gctgcccgtc
    19141 gacgccgtgg tagaaaaagc cgtcttcgaa aaggcgggcg agcttttcac gcactacttc
    19201 ttcgatctcg aagacttgat cgatttcatc ttgaacaaca gaggcataag gctcaagggc
    19261 gaggcctccg cggtggatcg gatacgcaac gccgcgaata ctttactgga ggttgtcgat
    19321 agtgattaga tcgaaaatcg tttatctatt tctctttctt ctgttcgccg cttttgcgtg
    19381 cgccggcaat ttgcgcgaac ttgtgtacgg ggaatcgctt ccggaaactc tggccgaagc
    19441 cggggcccgg gtcgtggata ctccgtcggc gggacttata aggatagaaa cccttctttt
    19501 caatcctttc aactggcagg gcatccaatg gcagaaccac ctgcttatca tccggcctcc
    19561 gagtgttcag tcgagagtgg ccctgatctt cataacgggc gactactctt attcggagga
    19621 tgagctggtg gtattccggc tgatggcgct ccagaacagg gcgtacgtgg ccgttctctt
    19681 cgacgttccc aaccagccgc tctttggagg aaagagggag gactggctga tcagccatac
    19741 tttccttgag tttctcagaa ccggtgatcc acggtggccg gcgctcgtac cgatggtcga
    19801 atcggctgta gtttcgatga acctaatcca tgattacgcc tcgagcagcg gcgacactgt
    19861 cgaggggttc gttctgagcg gaggatcgaa acgcgggtgg accacctggc tcacggcggc
    19921 gatcgatgag agggtggagg gcatcgttcc gatagcctac gacaacctca atctcgccga
    19981 ccagatgcgg caccagctgg acttctgggg aagctacagc cgttccatta gagagtacgt
    20041 ggatagcggc atactcaacg acctagacga tccgaagaag cgagaacttc tggaactggt
    20101 ggatcccttt acttacaggg attcgctcga tctgcccaag ctcatcgttg tgggaaccaa
    20161 cgatccgtac tggcccgtag atgccgccaa cctttatata gacgaccttc ccggttactc
    20221 cgccatggtg tacgcaccca acgccggtca cggcaccgaa gtttacaggg tcacccaggc
    20281 gatccagggg atgatctccc atctgaacac cgggctggaa tttccctcta tcgagatctt
    20341 tctcgaagag accgcctcga acgtgaaaat tagtttaaat gtcgatgaga gagactccaa
    20401 gctcgtggaa ttgcggcttt ttcactctct ctcggccgtc agggattttc gcaaggcctt
    20461 cttcgaatac gaaacgatcg ggaaagaaga ggagataatc gtcgatagag tctcattcaa
    20521 cgctttctac atagagggag ttttcacttt caacggcaaa gagctgctga tctctacacc
    20581 cgtaaaagta atcgaagcgc cctgatcggg cgcttcgaat ttgtcgatcc ggctggtttt
    20641 acgaaccgga ggaatccatt atcttctcgc tgttgaatac cttcgccagc gcgaatatca
    20701 ggaccgaaac atatgtcagg ttgctcaaga gcataagtaa aaagcgccag ctcacgaact
    20761 gcaaggttat tatgtccttc atgacgaata tcgagtttat gagcgggatg atatacagga
    20821 gcgggttgtc cggcgattcc atttgcatgg tggcgacgcc caggactacg gcgaaaatgt
    20881 agatcggaag tatgtagcca ccgccctcct ttatgtttct ggccagcgaa ccgaggagca
    20941 cgatgatcga agcggccagc cccgccatag tgagcagcgt tacgaaaagc ccggccagag
    21001 tggaaggtga aagagacgag aagttcatct ctccggcgcc gaaggccggt cctcccagtc
    21061 tgaaagcgat tatcaggccg atgaaggtga agacgctgtt gagcaccgcg atcatcatca
    21121 catagaggat tttgcccatc gctatcgaag t
//
LOCUS       DAOOLY010000009        20698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_2535_length_20698_cov_5.144407, whole genome shotgun sequence.
ACCESSION   DAOOLY010000009 DAOOLY010000000
VERSION     DAOOLY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_20698_cov_5.144407"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..2258)
                     /gene="rnr"
                     /locus_tag="PLD15_00965"
     CDS             complement(57..2258)
                     /gene="rnr"
                     /locus_tag="PLD15_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPB62495.1"
                     /translation="MKLTLKSLRTHFNSPEYTPGTLKSLALQLGASDKTSKSELKTLL
                     EEMTGTGEIVRDSRGRYIVPGSNNLTGIIEFTRRGLGAFVIEPGGEEIFIPPEEAGFA
                     IHGDTVMVEVTGDYKDIHRGRVIKVLKRNKTRVVGTFKTRGIFAFVVPDDVKLKNDFY
                     IPPEEFNGAKLDDKVIIKITKYPTPGRNPEGSVERVLGDSSAPEVDLMTVIYRHDLPE
                     PDEFPQEVSAQAKKISSTIPERDLKGRKDFRSEIVYTIDGEDAKDFDDAISLVRLENG
                     NYLLGVHIADVSHYVFEGSAIDREAFSRGTSVYLLDTVIPMLPFELSNNICSLKPDED
                     RLTLSLIMELDPRGKLLSSKVERGVIRSKRRLTYTEVNEFLEGTASRETLEKLEPVSG
                     SLLLSHELAGKIRENRKKRGSVMDISSREVKIVFSDTGRVLDIIPQSRGVSEWIIEEF
                     MILANETVAELFNDRGLPFVYRIHEEPDAESMMQLKNYLEALGMNVKLPRNVQPKALQ
                     QILEKTKDHPLHASIERVLVRGMKRAVYSENNIGHFGLASQAYTHFTSPIRRYPDLVV
                     HRLLKVFSSRGNGELTDEQREYEENLPSIASWASKRERVANEAEWDLLDMKKVEYISN
                     HIGEVFQGVITGLTKFGIFVEITEKMISGLVHISTLDDYYDFIENQNILVGRKNKRIF
                     RIGDDLKVQAVRADKTTMEIDFNIVEKEKKPVKKTSHEQKTRSKKPKKRHK"
     gene            2356..3378
                     /locus_tag="PLD15_00970"
     CDS             2356..3378
                     /locus_tag="PLD15_00970"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPB62496.1"
                     /translation="MRIGILTGGGDCPGLNAVIRGIVKSAPDNVEIIGIMHGWKGLVQ
                     GEYMKLTGDDVEGIHILGGTILGTARINPFRSEEMRIKLEENIKKLGLDVIIGIGGDD
                     TLSVTAKLASMGYRTVGVPKTIDNDVSNTDYTFGYHTAVNVGADAIDRLHSTAKSHGR
                     VIFVELMGREAGWITLEAGLAAGAHLILIPEYPMTIDEIMGYINRRMKDKGYMIIATA
                     EGFKPTELENVVGDKNQIDAFGHIKLGGIAHYMAEVVGERTGYDTRSMVLGYLLRGGT
                     PTAFDRILGTRYGVHAMELVIKGDFGRMVALRGNDIVSIPLEYGIATKKLVPYEYYKL
                     AQIFFG"
     gene            3388..3903
                     /gene="amrA"
                     /locus_tag="PLD15_00975"
     CDS             3388..3903
                     /gene="amrA"
                     /locus_tag="PLD15_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPB62497.1"
                     /translation="MENSEPVRLARYAIEERVTNGRVLSIPEWVSRELRTMMAGCFVS
                     IHKSDGALRGCIGTLSPVTASLASEIIQNAVSSATRDPRFPPVEAEELDELEITVDVL
                     GEAEATAAEELDPKRYGVIVQHGWKKGVLLPDLEGVDTVDEQLRIALMKAGIGKSESY
                     RIYRFTVRRYH"
     gene            3925..4920
                     /gene="amrS"
                     /locus_tag="PLD15_00980"
     CDS             3925..4920
                     /gene="amrS"
                     /locus_tag="PLD15_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPB62498.1"
                     /translation="MKPAVYFDEYDEGSTLQCLLCPRHCVIKQGEVGFCKTRRNVDGM
                     LYSLNYGQLTSIAVDPIEKKPLFHFFPGETILSVGSWGCNLNCEFCQNWEISKDRPKV
                     LKHVMPQQLIQIATERKSKGIAFTYNEPIVSFEFILDTSRAASKEGIYSVLVTNGVIE
                     REPMELLAQSMRAMNIDIKGWNDDFYKKEIGTDKANVLRSIEIALKSGVHMELTTLII
                     PGKNDSPEEMEEEALWIKNLSPDIPLHINRYFPNYHYTAPATEVESLLELASKAKEHL
                     NYVYVGNVALEGYNDTLCPNCGNLLVERRDYNVVVSGVDEKGRCTSCQREISLVF"
     gene            4881..5930
                     /locus_tag="PLD15_00985"
     CDS             4881..5930
                     /locus_tag="PLD15_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPB62499.1"
                     /translation="MHQLSKRNIASFLKGRVLFYGILLVAAAAILTYLFLREKPVEYY
                     DRTEYYLGTYVTVRVGSKTLSPLVLADAAMKEIKRIDEKFGGRGSGTIAQLNATGQAN
                     DLDNETMFLIKSALKVAENTGGAFDPTLFTLTALWGFDYADSENRVPLDSEIVSALAK
                     TGYRRVELDENNMAVRLPEGLTLDLGGIAKGYAVDLAIARIKSLDPQATGFIDAGGDI
                     GVIGPKYGNRPWIVGIRDPRGNSVQDVVEYIYLYDGAVATSGDYERFFVVDGIRYHHI
                     LDPKTGSPSRSGAISATVINKSAMLADAYATAAFVMGKDPGITFLPRYGTLVFLVMED
                     RSTFKSPGFEVYQQK"
     gene            5927..6274
                     /locus_tag="PLD15_00990"
     CDS             5927..6274
                     /locus_tag="PLD15_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPB62500.1"
                     /translation="MKFLGKFDWLIIVILILAAIILFIPHGQSAPIAEIYHDGRLVMT
                     VDLRVDSHFSLEGMEIAVSGGRISVVDSDCPDRICVNQGAIDRPGIPIVCVPNRVVIK
                     IPAGDTQFDAITQ"
     gene            6338..6871
                     /locus_tag="PLD15_00995"
     CDS             6338..6871
                     /locus_tag="PLD15_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPB62501.1"
                     /translation="MTYRDSDAQTAKRITTLSMLVAMGSVMYLLESLIPFPLPVPGGR
                     WGFSNLVLLLAVNGLSLRDTLYLAIGKTLVGGLLTGRLGTPGFIMGISGIVASALLMW
                     LLSRSRLFGMAGISLAGAAVSNLVQLFVAAWLIVRSGEILFLYPYMLLLGSISALINA
                     YIAHEVIRRIGDTIWFD"
     gene            6859..7422
                     /locus_tag="PLD15_01000"
     CDS             6859..7422
                     /locus_tag="PLD15_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPB62502.1"
                     /translation="MVRLILASSSPRRKELLLLLRVAFETVPPVGVKEELSASFDPDE
                     LADLSFRKARNVYERHPDAVVIGADTVVVLENTVLGKPSSKDEAFEMLVRLSEKMHYV
                     FTAVSVLERRWNFSFIERTAVKFRKIPPALIGEYVQTGIPLDKAGAYGIQDYGALFTE
                     IINGDYYNVMGLPIGRLWQELNTRGVI"
     gene            7423..8124
                     /gene="radC"
                     /locus_tag="PLD15_01005"
     CDS             7423..8124
                     /gene="radC"
                     /locus_tag="PLD15_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPB62503.1"
                     /translation="MEDTVLMPREKLLELGAERLTNEELVAILLRTGVKGKGVMEFSK
                     ELLNRYGSLTALLCADVREVMNVKGMGLAKAVLFKASLELGHRVFKEMSKKTRMKLDE
                     PESVYYLCHDMTLLDRETVRVISLDTKLNYCGLNTVSVGILDSSLLHPREVFKPAISR
                     TAAAIILVHNHPSGDPSPSKEDGNITNRIKEAGETLGIKLLDHIIVGKGSFFSFTAGR
                     VFYTEVGDDFQRKGL"
     gene            8099..8647
                     /locus_tag="PLD15_01010"
     CDS             8099..8647
                     /locus_tag="PLD15_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPB62504.1"
                     /translation="MISRERAYSLVEEHLKSKNLVKHVIATEAVMRALAVRLGQDPDS
                     WGMAGLLHDLDYEFTKERFEEHALKTVEMLADEDVSTEIKDAILAHCERKERQALIEK
                     AIYAADPVTGFIVAAVLIRKGARLADIDVEFLLNRFKEKSFARGASREQMASCEELGL
                     SLEEFLDVSLGAMQNISAELGL"
     gene            8695..9444
                     /gene="deoC"
                     /locus_tag="PLD15_01015"
     CDS             8695..9444
                     /gene="deoC"
                     /locus_tag="PLD15_01015"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPB62505.1"
                     /translation="MIEKIIESEMTRYRENYVPTVREVAEEIELAGYIDHTLLKADAT
                     PEMIVRLCDEAREFNFWSVCVNTGYLSLVSKALEGSPVKKTVVVGFPLGQMATMTKGA
                     ETVWALENGADEFDMVINVGLLKAREYQKVYEDISEVVESAEGKIVKVILENCLLTEE
                     EKVAACAISREAGAAFVKTSTGFSTGGATIEDVSLMKFVVGDRCRVKASGGVRTREDT
                     IAMIRAGADRIGTSSGIKIVTGGIARDKGGY"
     gene            9446..10075
                     /locus_tag="PLD15_01020"
     CDS             9446..10075
                     /locus_tag="PLD15_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPB62506.1"
                     /translation="MDFCALSVKDFLKKVAEKSPTPGGGAVGAVVAALAASLGSMVAN
                     LTIGKKGYEDVEGHMESALEICEAESVYLCELVNKDIQAFDQVMSAYKLPKATDEEKN
                     AREMKIQQALKTAIEVPFDLARRCKNIIVSIERLAKWGNLNVLSDAESAAYLLMAVYK
                     IAYANVMINMKSLKDAEYGAWISDEMAQLDRQMTATYDRIMDVLGKRNG"
     gene            10068..11213
                     /gene="tgt"
                     /locus_tag="PLD15_01025"
     CDS             10068..11213
                     /gene="tgt"
                     /locus_tag="PLD15_01025"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB62507.1"
                     /translation="MVELKLIKKSNECKARLGRLCTDHGEIDTPVFMPVGTNGTVKGL
                     WQSQLESLQAGIILGNAFHLYLRPGLNVLREFGGLHRFMSWPHSILTDSGGFQVFSLS
                     DKKILEEGVKFRSPLDGSQIFMTPELSMEIQQAIGSDIAMAFDECVEPGASREYVEDS
                     VGRTTRWARRCIEAHRESGQALFGIVQGGFFEDLRERSAAEITSMDFDGFALGGLSVG
                     EEFPITTRILSHSVDLLPSDRARYLMGIGSPDIILAAVENGIDMFDCVLPTRMGRHGT
                     ALTWTGRVNLKAARNRFDRSPVDEECSCAVCSTYSRAYIHHLLSRDEMLGKILLSYHN
                     ISFLMEFVRELREAIAEERYMDFKTECFRNGLIGSDSLESFSSERMG"
     gene            11218..12045
                     /locus_tag="PLD15_01030"
     CDS             11218..12045
                     /locus_tag="PLD15_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62508.1"
                     /translation="MKRSLVFFFVLFGVISLALNTFNLQTGGNGVISTGFNSGYGSPS
                     DLYGIVNSAFSTNFMEITRSASPLVNERHLYVQLFEDSDDNMAGALQYYMDIGPGSSL
                     RSLSYTISGNLGALTTYGADLKVNMHVATATTYDFALKGGISGKAFKLLDYVAAFEST
                     IWSSGLNKNIVDLLVGVRFVPDPFVIGLELGTRNGMEIRYFGLSTQFTYNKMFSVRGG
                     VSMNIDLENNMDFIIGGGIDVMIGNMLITGGIGTNLNRKIPSIAFEKTWNVAIMGKW"
     gene            complement(12202..12930)
                     /locus_tag="PLD15_01035"
     CDS             complement(12202..12930)
                     /locus_tag="PLD15_01035"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPB62509.1"
                     /translation="MRQKTIRLIDLIRDAIFPEIAAGPPGSEYESLSMAVDLLREVIS
                     QAQPAISIEKLERIAVDFVSELPAVAETLMKDVQAAYEGDPAAQSLEEIMVAYPAFEA
                     INIYRLAHILYLLGIPLIPRVMTEYAHQKTGIDIHPGASIGSYFFIDHGTGVVIGETC
                     TIGDHVKIYQGVTLGAKSFELDENGNPIKGIKRHPDIGNHVVIYAGATVLGGDTVIGD
                     NCTIGGNVWLIHSLEAGKTLYNNP"
     gene            complement(13025..13969)
                     /gene="cysK"
                     /locus_tag="PLD15_01040"
     CDS             complement(13025..13969)
                     /gene="cysK"
                     /locus_tag="PLD15_01040"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPB62510.1"
                     /translation="MPSIFTSIDQLIGRTPLLELVRTEKEYRLKAKILAKLECFNPAG
                     SVKDRIAKAMIDDAEEKGLLKEGSVIIEPTSGNTGIGLASVAAARGYRIIIVLPETMS
                     IERRMLMKAYGAELILTDGAEGMKGAIAKAKELAKDLPNSFIPGQFVNPANPEVHRKT
                     TGPEIWADTDGEIDCFVAGIGTGGTITGTGEYLKGLNPAIKVVGVEPATSAVLSKGIA
                     GSHNIQGIGAGFIPDVLNTKIYDEIIAVTDEDAFATGKALARREGILAGISSGAAAFA
                     AIEIAKRHESEGRTIVALFPDSGGRYLSTPMFMDYEND"
     gene            complement(14045..16066)
                     /locus_tag="PLD15_01045"
     CDS             complement(14045..16066)
                     /locus_tag="PLD15_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HPB62511.1"
                     /translation="MRNRSGTLLSIVIMLVTFVFANSTGTGLLNLNDSQKEATDKTAE
                     INRSYYSLLDFDDETEYEFATRGLIAAPETLELKDSNGRVVWSQKAYEFVDELETIPD
                     TANPSLWRDTVLNHCYGLFEVCEGIYQVRGYDLTNLTLIETESGGWIIFDPLMSLECT
                     EAAMKLVEENLGKRPVMAIVMSHPHADHFGGIRGVVREEEVADRALSIEEQISSGKVP
                     IIVPEGFTEHAVSENLFAGTAMARRAAYQYGVFLEKGVKDSLAMGIGLAQSTGTLTFI
                     LPTHEIKETGEKIVVDGVEMIFQMTPGTEAPAEMNTYFPQFKALWMAENCTGTLHNLY
                     TLRGAQVRDGNAWAKYILEAITLYGSETEVTFQSHNWPHWGNDVVIRYMEDTAAVYKF
                     IHDQTLMYINLGYTSDEISSMIRLPEYFEKVWYTRQYYGTVAHNSKAVYQKYMGWYDA
                     NPVHLNPLDPSERARKFVEYLGDTDRVLEMAYSDFLAGEYQWVAEITNILVFADPQNT
                     AARYLCADALEQLGYQSESGTWRNAYLTAALELRMGNSKDESKLAKGSTDILLNTTVE
                     MMLDYMGILIDSNAAEDIDITFNLVITDTNERYFVHLFHGVLLYYAGQTRELADATLT
                     CPKIGLLALINRNEAQQKLMIRVDGDGAIPMKLTQAMVAMDKTFNIVEP"
     gene            16625..17788
                     /locus_tag="PLD15_01050"
     CDS             16625..17788
                     /locus_tag="PLD15_01050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62512.1"
                     /translation="MSLRAMFVVVSSLIYFMFGFTGQSINSALPAIAIDLEMTHSMTG
                     LVLALPSLLYQVAVVVAAVLSRKTGPFLTMTAGLALVASSVAFMALSGTYVMFMTGRL
                     LFGLGLGATEISVALCVSLLAYEKTGGVLNLVYSFFALGSIVAPAVVSVFLTDTHRWN
                     IALFMALAAFAFVLSLSWYIGRKASGLSKRDGVKRKFSLPADSLFWLVVLGGLLYVGY
                     EMGVFSWLSSYVFEEKALELRISSFTPSLLALGLFVGRLVTGAVVDRAGLGRTLVILG
                     AFSLAGFSTVLLSVNPLVLTAGIFVAGLGFSGTFPTLQAILVSGYRERRETVLAVFAA
                     SGSLGVVITNISVGTVSEKYGLPNGMMIMAVQISAALAVFSIITFSQRAAHRE"
     gene            17881..18645
                     /gene="heR"
                     /locus_tag="PLD15_01055"
     CDS             17881..18645
                     /gene="heR"
                     /locus_tag="PLD15_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HPB62513.1"
                     /translation="MENVYKKLRVFNMIMAFLHLIQGVVMLILSNDSTLTITRNYLAF
                     DREIMKLVPATENLMNLRMGPFIASFLFMSSIAHFIVSTFGFKWYAKNLERGINYARW
                     YEYSISSSVMIVALAMFSGMLDIVSLMLLFTVNGLMNLFGLMMELHNQTTGKTDWTSF
                     MFGCVAGAVSWVAVFTYLFTSLGKANVQFPSFVYAIIVTFLVFFNSFAINMFLQYKKI
                     GPWKNYLFGENVYIILSLVSKSLLAWQVFAGTLRPV"
     gene            18659..19207
                     /locus_tag="PLD15_01060"
     CDS             18659..19207
                     /locus_tag="PLD15_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB62514.1"
                     /translation="MRVTLLNGAAPDSDAFDSYLERLAGNLRESGNTVSEFKLRDLVI
                     GQCIGCWGCWTKTPGECVFSDDTAKIARSAVDSDLFLFASPLVLGFTTSLLKKTIDKM
                     LPLVHPYLAVRAGEIHHRKRYRKYPLLGLLIARESDTDDIDISVVKEIYERVAIELAT
                     RLAFFGSTERPAEEVAHEIAGL"
     gene            19188..19871
                     /locus_tag="PLD15_01065"
     CDS             19188..19871
                     /locus_tag="PLD15_01065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPB62515.1"
                     /translation="MRLLVFNASPRGARSNSLAIIKHFLEGFEYSSREKPDIETVNLI
                     NERDDYAALREKLLVADRVVFAFPLYVDSMPGFLMEFFETLILLKGALSGKEFLFSIQ
                     CGFPETLNLRPLERYLVRLTGKFGAAYAGCICRGGMEGTRYMPADAPIFRKYRELGRI
                     YAETGKLDPGILGEIASPEKLPRPVMLIASVLGRMGLMNGYWKKQLKKNGAYEKRHAR
                     PYDPTHLPR"
     gene            complement(20100..>20698)
                     /gene="pncA"
                     /locus_tag="PLD15_01070"
     CDS             complement(20100..>20698)
                     /gene="pncA"
                     /locus_tag="PLD15_01070"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HPB62516.1"
                     /translation="ALLVVDVQNDFYETGSLPVKGASRINEVINRAMRDPVYTILAGQ
                     DWHPYNHMSFAANHGKEPFTPFDNKKGIGPVLWPFHCMQGTHGAEFHPDIESNRFDYI
                     VRKGTHPGVDSYSIFRENDGTELGTAGLLKALGIEELDICGLALDYCLKYTVHDALSA
                     GFKIKVIINGTKGVEANEGDVQRTLDEFKSAGVELIEK"
BASE COUNT         4870 a         5347 c         5539 g         4942 t
ORIGIN      
        1 cttcggccag cgtctttgtg atctgtaaaa agggcgggaa gttcccgccc ttccctttat
       61 ttatggcgct ttttcggctt tttcgacctg gttttctgtt cgtgactggt ttttttaacg
      121 ggctttttct ctttttcaac tatgttgaaa tctatctcca tggtcgtttt atcggccctg
      181 accgcctgaa ctttcaggtc gtcgccgatc ctgaagatcc tcttgttctt tctcccgacc
      241 agtatgttct ggttttctat gaagtcgtaa tagtcgtcca gagtggatat gtgcaccagg
      301 ccggatatca tcttttcggt tatttccacg aagattccga acttcgtgag accggttata
      361 acgccctgga aaacttcgcc tatgtggttg gagatatatt cgaccttctt catgtccagc
      421 aggtcccact cggcctcgtt ggcgactcgc tcccttttgg aagcccatga ggctatggag
      481 gggagatttt cctcgtactc tctctgttcg tcggtgagtt cgccgtttcc acgggaggag
      541 aagaccttca gcagtctatg gacgaccagg tcgggatacc tccttatagg cgaggtgaaa
      601 tgtgtgtaag cctgtgaggc cagaccaaag tggccgatat tgttttcgga gtagacggcg
      661 cgtttcatac ccctcaccag cactctctca atcgaggcgt ggagcggatg atcttttgtc
      721 ttctccagta tctgctgcaa cgctttaggt tgtacgttgc gcggaagttt aacgttcatg
      781 ccaagggctt cgaggtagtt cttcagctgc atcatcgact cggcgtcggg ttcctcgtgg
      841 attctataga cgaaggggag gccgcggtcg ttgaagagtt cggccaccgt ttcgttggcc
      901 agtatcatga actcctctat aatccattcg gaaactcccc gtgactgggg tataatgtcg
      961 agcacccggc ctgtgtcgct gaagacgatc ttgacctcgc gcgaggatat atccataacc
     1021 gagccgcgct ttttgcggtt ctcccggatc ttgccggcca gttcgtgcga gagcaggaga
     1081 ctcccgctca ccggttcgag tttttcaagc gtctctctgg aggccgtgcc ctcgagaaac
     1141 tcgttcacct cagtgtacgt gagccgtctt ttagaacgga taacgccgcg ctcaaccttc
     1201 gaggaaagca gtttcccgcg cgggtcgagc tccatgataa gagaaagcgt cagtctgtct
     1261 tcgtctggtt ttagcgagca gatgttgttc gagagctcga agggcagcat cggaattacc
     1321 gtgtccagca gatagacgga agttccccgc gagaaggcct ctctgtctat cgccgagcct
     1381 tcaaagacat aatgcgagac gtcggctatg tgtactccga gcaggtagtt cccgttttcc
     1441 agacggacca gtgagatggc gtcgtcgaag tccttggcgt cttcgccgtc gatcgtgtac
     1501 acgatctcgc tcctgaaatc cttccggcct ttcagatctc tttccggaat cgtcgaggag
     1561 atcttcttcg cctgtgcgct cacttcctgc gggaactcat cgggttcggg aaggtcgtgg
     1621 cggtatatca ccgtcatgag atcgacctcc ggagcgctag agtcgcctag aaccctctcg
     1681 accgaccctt cgggatttct gcccggcgtg gggtatttgg tgattttgat tatcactttg
     1741 tcgtcgagtt tggcaccgtt gaactcctcg gggggaatgt aaaagtcgtt ctttaacttc
     1801 acatcgtccg gtaccacgaa ggcgaagatc cccctggtct tgaaagttcc cacaaccctg
     1861 gttttgtttc gcttgaggac cttgattacc cttcccctgt ggatgtcttt gtagtcgccc
     1921 gtaacttcta ccatcacggt atctccgtga atcgcgaagc ctgcctcttc gggcggaatg
     1981 aagatctcct ctcctccagg ttcgataacg aaggcgccaa gaccgcgcct ggtaaattct
     2041 atgatgccgg tcagattgtt gcttccagga acgatgtatc tgcccctgga atcgcgcacg
     2101 atttcacccg taccggtcat ctcctcgagc agggttttca gttcgctctt cgatgttttg
     2161 tcgcttgctc ccagttgcaa agccaggctc ttcagcgtcc cgggagtgta ttcgggtgag
     2221 ttgaagtgtg ttcgcagtga tttcaaagtt agcttcatta gcctctttcc tttcgttttt
     2281 acctgaattt cgtacctgtt tatgatataa ttatacagtg tctttcgtta tatctattca
     2341 aacaggaggt tcgcaatgcg aattggaata ttgaccggtg gcggggactg ccctgggctg
     2401 aacgccgtta ttagaggaat agtgaaatct gcacccgata acgtggaaat cataggaata
     2461 atgcacggct ggaagggact cgttcaggga gagtacatga aactgaccgg agacgatgta
     2521 gagggcatac acatcctcgg cggaacgatt ctgggcacgg cgagaatcaa tcccttcagg
     2581 tcggaggaaa tgagaatcaa gctcgaggaa aatataaaga agctggggct ggacgtcata
     2641 atcggcatcg gcggcgacga cacgctgagc gtcacggcca agctggcctc catgggatac
     2701 aggacggtcg gcgtacccaa gacgatagac aacgacgttt ccaacaccga ctacactttc
     2761 ggttaccaca cggccgtcaa cgttggagcc gatgctatcg acagactgca ttcgaccgcc
     2821 aaatcccacg gaagggtgat attcgtcgag cttatgggac gcgaagccgg ctggataact
     2881 ctcgaagccg gactggccgc cggggctcac ctgatactga ttcccgaata ccccatgact
     2941 atcgacgaaa taatgggata tatcaacaga aggatgaagg acaaaggtta catgataatc
     3001 gccacggccg agggtttcaa acccaccgag ctggagaacg tcgtgggcga caagaaccag
     3061 atagatgcct tcggccacat aaagctcggc ggaatagccc actatatggc cgaagtggtg
     3121 ggagagagga ccggatacga cacgcgatcg atggttctgg gataccttct gagaggtgga
     3181 acgccgacgg cctttgacag gatactcggc accaggtacg gtgttcacgc catggaactg
     3241 gttatcaagg gcgacttcgg aagaatggtg gccctcaggg gtaacgatat agtgtcgata
     3301 cctctggaat acggaatagc cactaagaag ctcgttcctt acgaatatta caaacttgca
     3361 cagatattct ttggttgagg gacgtttatg gaaaactctg agccggtaag gctcgccaga
     3421 tatgcgattg aggagcgtgt cacgaacgga cgcgttctct ctatacccga atgggtttcc
     3481 cgggaactca gaacgatgat ggccggatgc ttcgtctcga tccacaaaag tgacggagcc
     3541 ctgagaggct gtataggcac gctctcgccc gtaacggcga gcctggcgag cgagataata
     3601 caaaacgccg tatcttcggc gacccgtgac ccgagattcc caccggtcga ggccgaagaa
     3661 ctggacgaac tcgagataac cgtcgacgtg cttggagagg ccgaagcgac tgccgccgaa
     3721 gaactcgacc cgaaacggta cggagtgatc gtccagcacg gctggaagaa gggagtactc
     3781 ctccctgacc tggagggggt ggacacagtc gacgaacaac tcaggatcgc cctcatgaag
     3841 gcgggtatag gcaagagcga gagctaccga atatatagat tcaccgtaag aaggtatcat
     3901 tagataacct gagaggtgat ttttgtgaaa ccggccgtat atttcgacga gtacgacgaa
     3961 gggagtactc tccagtgtct attgtgcccc cgtcattgcg tgataaagca gggcgaagta
     4021 ggcttctgca aaacgaggag aaacgttgac ggaatgctgt actccctgaa ctacgggcag
     4081 ttgacctcga tcgccgtaga cccgatagaa aagaagccgc tctttcactt ctttccggga
     4141 gagacgatac tctccgtcgg ttcctggggc tgcaatttga actgtgaatt ctgccagaac
     4201 tgggagatct ccaaggacag accgaaggta ctcaagcacg tcatgcccca gcagctgatc
     4261 cagatagcca ccgaaagaaa atccaaaggg atcgccttca cctacaacga acccatagtc
     4321 tctttcgaat tcattctcga cacatcgcgc gccgcatcca aagaggggat atacagtgtg
     4381 ctcgtcacca acggcgttat agagagagaa cccatggagc ttctggccca gtcgatgagg
     4441 gcgatgaata tagatataaa aggctggaac gatgatttct acaaaaagga aatcggaacc
     4501 gacaaagcaa acgtacttcg gtccatcgag atcgcactca agtccggtgt gcacatggaa
     4561 ctcacgaccc tgatcatacc cgggaagaac gacagtcccg aagagatgga agaagaggct
     4621 ttgtggataa agaacctgtc gccggacata ccgctccaca taaacagata ctttcccaat
     4681 taccactaca cagccccggc aactgaggtc gaatcgcttc tagagctggc ttcgaaggcg
     4741 aaggaacacc tgaactacgt ttacgtgggc aacgtcgcac tcgaaggcta caacgatacg
     4801 ttgtgtccta actgcggaaa cctgctcgta gagagaaggg actacaacgt tgtcgtgagt
     4861 ggagtggatg aaaaaggcag gtgcaccagt tgtcaaagag aaatatcgct agttttctga
     4921 aaggcagagt cctcttttac ggaatccttc tggtcgcggc cgccgcgatc ttgacctacc
     4981 tcttcctcag ggaaaagccg gtagagtact acgacaggac cgagtactac ctgggaacct
     5041 acgtaacggt gagagtcggc tcgaaaacac tctcaccgct ggtgctggcc gatgcggcca
     5101 tgaaagaaat aaagaggatc gacgaaaagt tcggcggcag aggctcgggg acgatcgctc
     5161 aactgaacgc gaccggccag gccaacgatc tcgacaatga gacgatgttt ctcatcaagt
     5221 cggcgctgaa agtggccgaa aacaccggtg gcgccttcga tccaacgctc ttcacgctga
     5281 ccgccctatg gggcttcgac tatgccgact ccgaaaatag agttcctctc gacagcgaaa
     5341 tagtttcggc actcgcgaaa accggttacc gaagagtgga gcttgacgaa aataacatgg
     5401 ccgtcaggct gcctgaaggg ctaacccttg atctgggcgg gatcgccaag ggttacgccg
     5461 tggatctggc gatcgccaga ataaaatcgc tcgatcccca ggccacaggc ttcatagacg
     5521 ccggtggcga tatcggcgtt atcgggccca aatacggcaa caggccctgg atagtcggaa
     5581 tacgcgatcc caggggaaat agcgtacagg acgtggtaga gtacatatac ctctacgacg
     5641 gcgccgtggc cacctcgggc gactacgaaa ggtttttcgt cgtcgatggc ataaggtacc
     5701 accacatact cgacccgaaa actggcagcc cttcgaggag cggagcgata agcgccacgg
     5761 taataaacaa aagcgcgatg ctcgccgacg cctacgcgac agcggccttc gtgatgggta
     5821 aagacccggg aataaccttc cttcccaggt acggtacgct cgtctttctg gtgatggagg
     5881 atcgaagcac cttcaaatcg ccgggattcg aggtctatca gcagaaatga agtttctcgg
     5941 caagttcgac tggctcataa ttgtcatact gatcctggcg gcgatcatac tcttcatccc
     6001 gcatggacaa tcggccccga tagcggagat ctaccacgac gggagactgg tgatgacggt
     6061 cgatcttcgc gtggattcac atttcagcct ggagggcatg gaaatcgccg tgagtggcgg
     6121 cagaatctcg gtcgtcgatt ccgactgccc agacaggatc tgcgtgaacc agggagccat
     6181 cgacaggccg ggaattccga tcgtctgcgt tcccaacagg gtagtgataa agattccggc
     6241 aggcgatacg cagttcgacg cgataactca gtgaagagaa aggacagagg caggccagta
     6301 cgcggcgcga ctggataatc tgaacggtgg acgggctatg acatatcgcg attcagacgc
     6361 tcaaaccgca aagagaataa cgacgctttc catgctggta gccatgggct cggtgatgta
     6421 ccttctcgaa agtttaatac cctttccgct gcctgtccct ggcggaagat ggggcttctc
     6481 gaacctcgtg cttctgctgg ccgtcaacgg cctctccctg cgcgataccc tgtatctggc
     6541 cataggaaag acactcgtag gaggacttct caccggtcgt ctgggtacgc cgggcttcat
     6601 catgggaata tctggaatag tcgcctcggc gctcctgatg tggctcctct ccagaagccg
     6661 gttattcggc atggcaggta tcagccttgc cggcgccgcc gttagcaacc tcgtgcagct
     6721 attcgtcgcc gcctggctta tagtgaggag cggggagata ctcttcctct atccctacat
     6781 gcttctgctc ggttcgatct cggccctcat aaacgcctac atagcacacg aagtgataag
     6841 aaggataggt gataccatat ggttcgactg atactggctt cctcttcgcc gcgccgcaaa
     6901 gaactgctgc tactcctcag agtcgccttc gagaccgtcc cacccgtcgg agtgaaggaa
     6961 gaactctccg cgtccttcga cccggacgaa ctcgcggacc tctccttcag aaaggcgcgg
     7021 aacgtttacg aaagacatcc cgacgcggtg gtcatagggg ccgacactgt cgtagtgctc
     7081 gagaacaccg tcctcggaaa accatcatcg aaggatgagg ctttcgaaat gctggtgcgg
     7141 ctctcggaaa agatgcatta cgtcttcacg gccgtatcgg tgctcgagcg tcgatggaat
     7201 ttcagtttca tagagcgaac cgcagtcaaa ttccgcaaga taccgcccgc gctgatcggg
     7261 gaatacgtcc agacgggcat cccactcgac aaggccgggg catatggaat acaggactac
     7321 ggcgctcttt tcaccgaaat cataaacggg gattattaca acgtaatggg gttgcccatc
     7381 ggtagattgt ggcaggaact aaataccaga ggggtgatct agttggaaga cacggtactg
     7441 atgcccaggg agaaacttct cgaactcggg gcggaaagac tcacgaacga agaactcgta
     7501 gccatccttt tgagaacggg ggtaaagggg aagggtgtga tggaattcag caaagaactc
     7561 ctgaaccggt acggatcgct cacggcgctg ctctgcgccg acgtgaggga agtgatgaac
     7621 gtcaagggaa tgggcttggc caaggcggtt ctcttcaagg cctccctgga gcttggacac
     7681 agggttttca aagagatgtc caaaaagacc cggatgaaac tcgacgaacc ggaaagcgtt
     7741 tattatctct gccacgatat gacactgctg gacagagaaa cggtgagggt gatctcgctc
     7801 gacaccaagt tgaattattg tggtctcaac accgtcagcg tgggaatact cgattcctcg
     7861 ctcctgcacc cgcgcgaagt cttcaaaccg gccatcagca gaacggcggc tgcgatcatt
     7921 ctcgtgcaca accatccgtc gggagatccc agtcccagca aagaagatgg taatataaca
     7981 aatagaatta aagaagccgg cgaaaccttg ggtataaagc tcctggatca tataattgta
     8041 gggaaaggca gttttttcag ttttacggcg ggaagggtat tttacacgga ggtgggcgat
     8101 gatttccaga gaaagggctt atagtctggt agaggaacat ttgaaatcga aaaacctggt
     8161 caaacacgtg atagccaccg aggcggtgat gcgtgcgctg gccgtcaggc tcggtcagga
     8221 tccggatagc tggggcatgg ccggtctgct ccacgatctc gactacgaat tcaccaaaga
     8281 acgcttcgaa gaacacgctt tgaagaccgt cgagatgctc gccgacgagg acgtctcgac
     8341 cgagataaag gacgcaatcc tcgcccactg cgagagaaaa gaaaggcaag cgcttatcga
     8401 gaaggccatc tacgcggccg atcccgtaac cggcttcata gtcgccgccg tcctcatacg
     8461 gaaaggagcc agactggccg atatcgacgt ggagttcctg ctcaacagat tcaaagagaa
     8521 gtctttcgcc aggggagcca gcagggaaca aatggccagc tgtgaagaac tcggcctgtc
     8581 gctcgaggag tttcttgacg tatctctcgg cgcgatgcaa aatatttccg cagaactggg
     8641 gttgtaattg tagggtatgg aaatggcata atagattgaa agggagtgtt tccagtgatc
     8701 gagaagatca tagaatcgga aatgacaaga tacagggaaa actatgtacc gaccgtgaga
     8761 gaggtggccg aagaaatcga actggccggg tacatagacc acactctcct gaaggccgac
     8821 gctacaccgg agatgatcgt caggctctgc gatgaggcca gagagttcaa tttctggagt
     8881 gtctgtgtca ataccggata tctctcgctc gtcagtaagg cgctcgaagg ttcgcccgtg
     8941 aagaagacgg tcgttgtagg ctttcctctg ggacagatgg ctacgatgac caagggcgcc
     9001 gagacggtgt gggctctgga aaacggggcc gatgagttcg atatggtgat caacgtcggg
     9061 ttactgaagg cgagagagta tcagaaggtt tatgaagata tatcggaagt ggtagaatcg
     9121 gcagaaggca aaatcgtcaa agtcattctc gaaaactgtc ttttgacgga agaagaaaaa
     9181 gtggccgcct gcgctatttc gagggaggcc ggtgccgctt ttgtcaaaac atcgactggc
     9241 ttttccaccg gcggggcgac gatcgaggac gtttcgctga tgaaattcgt cgtcggtgac
     9301 cgttgcaggg tcaaggcttc cggcggagtc agaaccagag aggacaccat agccatgatc
     9361 agggccggag ccgatagaat aggcaccagt tcgggcataa aaatagtgac tggcgggatc
     9421 gccagagata aagggggata ttgagatgga tttttgcgct ctttcagtaa aggattttct
     9481 taagaaggtg gcggaaaaga gtccgacacc gggtggcgga gcggtcggag ctgtggtggc
     9541 ggcgctggcg gcgtctctgg gctctatggt ggccaacctc acgataggca aaaaaggata
     9601 cgaagatgtg gaaggccata tggaatcggc actggagatc tgtgaagccg agtccgttta
     9661 tctttgcgaa ctggtgaaca aggacattca ggcattcgat caggtaatgt ccgcatacaa
     9721 gcttcccaaa gccaccgacg aagaaaaaaa cgcgagggaa atgaagatac agcaagccct
     9781 gaagaccgca atagaggtgc cttttgatct ggccagaaga tgcaagaata tcatagtcag
     9841 catagaaagg ctggccaagt ggggcaacct caacgttcta tcggacgccg agagcgccgc
     9901 gtacctgcta atggcggttt acaagattgc ctacgccaat gtaatgataa acatgaagtc
     9961 cctgaaggat gcggagtacg gcgcctggat ctccgacgag atggcccagc tagacaggca
    10021 gatgaccgca acctatgaca ggataatgga cgtactcgga aaacgtaatg gttgaactga
    10081 aattaataaa gaaaagcaac gaatgcaagg cccggcttgg aagactgtgt accgatcacg
    10141 gtgagataga cacccccgtc ttcatgccgg tgggaacgaa cggaaccgtc aagggactct
    10201 ggcagagcca gctggagtcc ctccaggcgg gcattatact gggaaatgcc tttcacctgt
    10261 atcttagacc gggtctgaat gttttgagag agttcggcgg gcttcaccgg ttcatgtcct
    10321 ggccccactc gatcctgacc gacagcggcg gcttccaggt tttttctctg agcgacaaga
    10381 agatactgga agagggagtg aagtttcgct caccgctcga cggcagccag atattcatga
    10441 ctcccgaact ctcgatggaa atccagcagg cgataggctc ggacatcgcg atggccttcg
    10501 acgagtgcgt cgagccgggt gcttcgagag aatacgtcga agactccgtg ggaagaacca
    10561 cgcgctgggc ccgccgctgt atagaggcac acagggaaag cgggcaggca cttttcggta
    10621 tcgttcaggg cggtttcttc gaagatctga gggagaggag cgccgccgag atcacgtcga
    10681 tggatttcga cgggttcgcg ctcggcgggc tgagtgtggg agaggagttc cctatcacta
    10741 ccaggatact ctcacattcg gtcgatctcc ttccctccga tagagccagg tatctcatgg
    10801 ggatcggctc gccggacata attctggccg ccgtggaaaa cggcattgat atgtttgatt
    10861 gcgtacttcc gacgaggatg ggaaggcacg gcacggcgct gacctggaca ggaagggtca
    10921 acctcaaggc cgcgaggaac aggttcgaca ggtctccggt cgatgaagag tgttcctgcg
    10981 ccgtttgttc gacttacagc agggcttaca tacatcatct cctctccaga gacgagatgc
    11041 ttggcaagat cctgttgagt tatcacaaca taagttttct gatggagttc gtcagagagc
    11101 tgcgagaagc gatagccgaa gaaagatata tggatttcaa aaccgagtgc ttcaggaatg
    11161 gattgatcgg gagtgatagt ctcgaaagtt tttccagcga gcggatgggg tgatatgatg
    11221 aagaggtctc ttgtcttttt ctttgtgctt ttcggtgtta tttctctggc cctgaatact
    11281 ttcaatcttc aaaccggcgg aaacggcgtc atatccacgg ggttcaactc cggctatggc
    11341 agtccttccg atctgtacgg gatagtgaac tcggcctttt ccacaaactt tatggagatt
    11401 accaggagcg cttcgccact tgtcaacgag cggcatctct acgtgcagct cttcgaagat
    11461 tccgacgaca acatggccgg cgccctgcag tactacatgg atataggtcc cggttccagt
    11521 cttcgttctc tgagctacac gatttccggc aatctgggcg ctctgaccac ctacggtgcc
    11581 gacttgaaag tcaatatgca cgtggcgacc gcaacgactt acgatttcgc gctgaagggc
    11641 ggtatatccg gaaaggcctt caaactgctg gattacgtgg cggccttcga aagcaccatc
    11701 tggtcgagtg gcttgaataa aaacatcgta gatttgctgg tcggcgtcag gttcgttccc
    11761 gatcccttcg tcataggtct ggaactggga acgagaaacg gcatggagat caggtatttc
    11821 ggtctgtcta cccagttcac ctacaacaaa atgttctcgg tgaggggcgg cgtctcaatg
    11881 aacatagacc tagagaacaa catggacttc ataatcggcg gcggtatcga cgtcatgatc
    11941 ggcaacatgc ttataactgg cggaataggg accaatctga acaggaagat cccttccata
    12001 gccttcgaga agacctggaa cgtagccata atgggcaaat ggtgatgtag ccttcaagca
    12061 ggtttcggtt ttccgcccgc gggcaaaacc agccttgcca cgactcccgg tttctggaag
    12121 cgtatttcga agccatagat tcgcccggcg aggatccggg ttttgatata aggatacggc
    12181 tagacgcatc cgaccttgcc gtcaagggtt gttgtacagt gttttccccg cctccagcga
    12241 atgaatcaac cagacattac ctccgatcgt gcaattatct ccaatgactg tatctccacc
    12301 gagcaccgtc gccccggcat aaatcacgac gtgattgccg atatccgggt gacgtttgat
    12361 tcccttgatg ggattgccgt tttcatccag ttcgaagctc ttcgctccaa gtgtcacacc
    12421 ctgatagatc tttacatgat ctcctatcgt acaggtttct ccaatgacaa cacctgtgcc
    12481 gtgatcaatg aaaaagtagc tgcctatgga cgctcccgga tgaatatcga tacctgtttt
    12541 ctgatgagcg tattcggtca ttacccgggg aattagaggg ataccgagta gatagagtat
    12601 atgcgccagc cggtagatgt tgatcgcttc gaaggcagga taagccacca ttatctcttc
    12661 aagagactgg gccgcagggt ccccctcgta cgctgcctgc acgtctttca taagggtttc
    12721 cgccacggcg ggcagttcgc tcacaaaatc cactgcgatg cgttccagct tttcgatgga
    12781 tatcgctggt tgcgcttgag agatcacctc tctcagcaga tccacggcca tcgacagaga
    12841 ttcgtactcc gacccgggtg ggccggcagc gatttcaggg aaaatcgcgt ctcgaatcag
    12901 gtcgatcagc cgtatagtct tctgcctcat actgcccagc gaacggagtc ctctggacag
    12961 gtgaagactg ttaaaccgtt tggatacctc ttcaacgcac tcaattaatt tatcgggttc
    13021 atgatcagtc attttcataa tccataaaca taggcgtaga taaatatctg ccaccggaat
    13081 ccgggaataa ggcaacgata gtccttccct cgctttcatg acgttttgcg atctcgatag
    13141 ccgcaaatgc agctgcgccg gaagaaatac ctgccaggat gccttccctt cttgcaaggg
    13201 cctttcctgt ggcgaaagca tcttcgtccg tgaccgcgat gatctcgtcg tagatctttg
    13261 tgttcaaaac atcaggaata aagcccgctc caattccctg gatattgtga gacccggcaa
    13321 tgcctttgga gaggaccgcc gaggttgcag gctccacacc cacaactttg attgcggggt
    13381 tcagtccctt cagatactcg ccggtacctg tgatcgttcc acccgtaccg atgccggcaa
    13441 cgaagcaatc gatttcaccg tcggtatcgg cccagatttc ggggccagtg gtttttcgat
    13501 gtacctcggg gtttgccgga ttgacgaatt gaccgggtat aaagctattc gggagatcct
    13561 tcgccagttc tttcgctttg gcgatggctc ccttcatacc ctccgcaccg tcggtcaata
    13621 tcagttctgc accataagcc ttcattagca tgcgacgttc aatcgacata gtttcgggca
    13681 aaacgatgat tatgcgatat ccgcgtgcgg cggcgactga agcaaggcct atgcccgtgt
    13741 tgccggaggt cggttcgata atcaccgagc cttcttttaa aaggcccttc tcttcggcat
    13801 catctatcat cgcctttgca attctatcct ttacggaacc ggccgggtta aaacactcca
    13861 gctttgcaag tatttttgct ttcagcctgt attccttctc ggtacgtact agttcaagta
    13921 acggggtcct gcctatcaac tgatcgatcg atgtgaaaat actgggcatg atctaaactc
    13981 cttaccatct cataaatcat agagtaaaaa accgccaccg gaactttcca gtggcggcca
    14041 ggttttacgg ttcgactata ttgaaggttt tatccattgc caccatcgct tgagtcaatt
    14101 tcatcggaat agcgccgtca ccgtcgactc tgatcatcaa cttctgttgc gcttcgttcc
    14161 tgtttatcag ggctaagaga ccgatcttcg ggcatgtcag ggtcgcgtcc gcgagctcgc
    14221 gtgtctgacc tgcgtaatag agcagcacac cgtggaaaag gtgtacgaag tacctctcgt
    14281 ttgtatcggt tataaccagg ttgaaagtga tgtcgatatc ctccgccgca ttcgaatcga
    14341 taagtattcc catgtaatct agcatcatct caacggtcgt attgaggaga atatcagtgc
    14401 ttcccttagc gagtttcgat tcgtccttcg aatttcccat gcgcaactcg agagccgcgg
    14461 taaggtaagc atttctccac gttcccgatt ccgactggta acccagttgc tcgagagcgt
    14521 cggcacatag ataacgcgcg gccgtgttct gcgggtctgc gaaaaccagg atattggtga
    14581 tctcggccac ccactggtat tcaccggcca ggaaatcgct gtaggccatt tccagaaccc
    14641 tgtcggtatc gcccagatac tcgacgaact ttctggctct ttccgacgga tcgagcggat
    14701 tcagatgtac ggggttggcg tcgtaccagc ccatgtactt ctggtaaacg gccttagagt
    14761 tgtgtgcgac ggtgccgtaa tactgccgcg tgtaccagac cttttcgaag tattccggca
    14821 gacgtatcat actggagatt tcgtcggaag tgtaaccgag atttatgtac atcagcgttt
    14881 gatcgtgtat gaacttgtaa acggcggccg tgtcttccat ataccttatg actacatcat
    14941 ttccccagtg aggccagttg tgcgattgaa aggtcacttc cgtttcagag ccgtaaagag
    15001 tgatcgcctc cagtatatac ttggcccagg cgttgccgtc gcgcacctgc gctccgcgca
    15061 gcgtgtaaag gttatggagc gttccggtac agttttcagc catccagagg gctttgaact
    15121 gcggaaaata cgtgttcatc tccgcggggg cttccgttcc cggagtcatc tggaatatca
    15181 tctcaacgcc gtcgacgact atcttctcgc cggtctcctt gatttcgtgg gtgggtaaga
    15241 tgaaggtcaa cgttcccgtc gattgcgcga gtcctatacc catggccaga gaatccttga
    15301 cgcctttttc cagaaataca ccgtactggt aggcggctct tctcgccatg gcggttccgg
    15361 cgaaaaggtt ctcgctcacg gcgtgttcgg tgaaaccttc gggaacgatt atgggaactt
    15421 tacccgagga gatctgctct tcgatcgaga gagctctatc tgcaacctct tcttcccgta
    15481 cgactcctct tattcctccg aaatggtctg catggggatg actcattact atggccatca
    15541 cgggacgctt gccgagattt tcttctacga gtttcatggc cgcttcggtg cattcgagac
    15601 tcatcaaggg atcgaagatt atccagccgc cgctttccgt ttcgatcaac gtgagatttg
    15661 taagatcgta gccgcgtacc tggtagatac cttcacagac ttcgaaaagc ccgtaacagt
    15721 ggttcaaaac agtgtctctc cacagactgg gattggccgt gtctggtatc gtctcgagtt
    15781 catcgacaaa ttcataggct ttctggctcc agacaacacg tccgttgctg tctttcaatt
    15841 cgagggtctc cggtgccgct ataagccccc tggttgcaaa ttcgtactcg gtttcgtcat
    15901 cgaaatcgag aagcgagtag taactccggt ttatttctgc ggttttatct gtagcctctt
    15961 tttgcgagtc gttcaaattc aacagaccag ttccggtgga atttgcaaac acaaacgtga
    16021 ccagcattat cacaatcgat agaagcgttc cggatctgtt tctcattccc tgcacctcct
    16081 gacttttaaa acacggcttg agactgtacg tgacttcctc tacatagaca acacacctta
    16141 aactctacag ctagcacaca gagcaaggct caacctattt gggcactatc acgggagaat
    16201 tttccccact acctgatacc gatcgttata accggcctgt tataaagcgt aattaacatg
    16261 acgttaactt caaacgacca caatttccag agccttcttt tgcagagata gccttaatag
    16321 taacctgtca agattgtaac tcctgataat aaacaacttt gatgactcat gatgatctac
    16381 attcagttga aattgtagca tttatgagaa tgttatttgc acgaatattt ggacagtctt
    16441 accgattctg ccttttaact tctcaacctg tgcatttcga tgattgtgtt ctattttctc
    16501 ctttttgtgc caaaatgctc tggagatact catgcttgat atgctaaact taatctctaa
    16561 aacgcaaata aagcgaaaac acgcttttct atcgctgaat cgcaggtgtt gatggaggca
    16621 tacgatgagc ttgagagcga tgttcgtggt tgtttcgtct cttatatact tcatgttcgg
    16681 cttcacgggt caatctataa acagcgccct tccggccata gcgatagacc tggaaatgac
    16741 ccattccatg accggtcttg tccttgccct cccgtcgctt ctctatcagg tcgcggtcgt
    16801 tgtcgcggcc gtcctttccc ggaaaaccgg ccctttcctg actatgacgg ccggcctggc
    16861 cctggtcgca tcttcggtgg ccttcatggc cctctccggg acttacgtta tgttcatgac
    16921 cggcaggctg cttttcggac tcggactggg agccacggag ataagcgttg cgctgtgcgt
    16981 ctctcttctg gcgtacgaga agaccggggg cgtgctaaac ttagtctatt ccttcttcgc
    17041 gctcggaagt atagtcgctc cggccgtcgt gtcggtcttt ctcacagata cgcatagatg
    17101 gaacatcgct cttttcatgg ccctggccgc cttcgcgttc gtgctgtcgc tttcatggta
    17161 catcggccgg aaagcttcgg gactttcaaa gagagacggc gtaaagcgga agttctctct
    17221 cccggcagat tcgcttttct ggctggtcgt tctgggaggt cttctgtacg tcggatatga
    17281 gatgggagtg ttttcgtggc tttcaagcta cgttttcgag gagaaggccc tggaactgag
    17341 gatttctagc tttaccccat cacttctcgc actcggtctg tttgtgggca ggctggtcac
    17401 cggcgcagtt gtagatagag ccggtctggg gagaaccctt gttatactgg gcgctttctc
    17461 gctggcgggc ttttcaactg tactcctttc ggtgaacccg ctcgttctga cggcgggaat
    17521 tttcgtcgcc ggcctcggtt tctccgggac ctttcccacc ctccaggcca tactggtttc
    17581 gggatacaga gagaggcgcg agacggtcct tgcagtcttc gccgcttccg gctctcttgg
    17641 agtggtcatc actaacattt ccgtgggaac cgtgagcgag aagtacggtc tgccgaacgg
    17701 catgatgatt atggcggtcc agatctcggc cgctctggcg gtgttttcaa tcatcacatt
    17761 ctcgcagaga gcggcacaca gagaatagac gatcgaccgg tgaagagtct ctatgaaaga
    17821 tcgtaaccgg tgcgtggagt gttaccattt agaggtaacc aaaaaaaggg ggatgaaagc
    17881 atggaaaacg tatataaaaa gctccgcgtc ttcaacatga taatggcctt cctgcacctg
    17941 atccagggag tagtcatgct gatcctgagc aacgactcga ccctcacgat cacgaggaat
    18001 tacctggcct tcgatcgtga aataatgaag ctcgttccgg caaccgagaa tcttatgaac
    18061 cttcggatgg gccccttcat agccagcttt ctcttcatgt cgtccatagc ccattttata
    18121 gtctcgactt tcggtttcaa gtggtacgcg aagaatctcg agcggggcat caattacgct
    18181 agatggtacg agtattcgat cagttcttcg gtgatgatcg tagcgctggc catgttttcc
    18241 gggatgctgg atatagtgag tctcatgctc ctcttcaccg tgaacggtct tatgaatctc
    18301 ttcggcctca tgatggaact tcacaaccag accacaggga agaccgactg gacttccttc
    18361 atgttcggct gtgtcgccgg agccgtctcg tgggtggccg ttttcacata cctattcacg
    18421 tccctgggca aggcgaacgt tcagtttccg tctttcgttt acgcgattat cgttaccttt
    18481 ctcgtcttct tcaacagctt cgcgataaat atgttccttc agtacaagaa gatcggtccg
    18541 tggaagaatt atctcttcgg ggagaacgtt tacataatac tcagtctggt ttccaagagt
    18601 cttctcgcct ggcaggtctt cgccggcacg ctccggccgg tatgataagg aagccggcat
    18661 gagggtcacg cttctgaacg gagccgcccc ggacagcgac gcattcgaca gttacctcga
    18721 aaggctcgcg ggaaatttga gagaatcggg gaacacggta agcgaattca agcttagaga
    18781 tctcgtgata ggccagtgca tcggctgctg gggctgctgg acgaaaacgc ccggagagtg
    18841 cgtcttctcc gacgataccg caaagatagc caggtcggcg gtcgactccg atctctttct
    18901 cttcgcctct cccctcgtcc tgggcttcac cacttcactg ctgaagaaga ccatagacaa
    18961 gatgcttccg ctggttcacc cttacttagc cgtaagggcc ggagagattc accacagaaa
    19021 acggtaccga aaatatcccc ttctgggatt gctgatcgcc cgggagagcg atacggacga
    19081 tatagacatc tctgtcgtga aggagatcta cgaacgcgtg gcgatcgaac tggccaccag
    19141 gctggccttc ttcggctcta cagaaaggcc ggcggaggag gttgcgcatg agattgctgg
    19201 tctttaacgc atcgcccagg ggtgccagaa gcaactcgct ggcgataatc aaacactttc
    19261 tcgaaggctt cgagtactct tcacgcgaaa agccggacat agagacagtg aacctcataa
    19321 atgaacgcga cgattacgct gccctccggg agaagcttct ggttgccgac agggtagttt
    19381 tcgccttccc gctttacgtt gacagtatgc cgggctttct tatggagttc ttcgaaactc
    19441 tgatactctt gaagggagcc ctttcgggca aggagtttct attctccatt cagtgcggct
    19501 ttcccgaaac tctcaacctt cgccctctcg agagatatct cgtaaggctc accggtaaat
    19561 tcggcgcggc ctatgcaggt tgcatatgcc ggggaggaat ggaggggacc agatacatgc
    19621 cggcagacgc acccatcttc cggaaatacc gggaactagg gaggatctac gccgaaaccg
    19681 gcaaactcga ccccgggatt ctcggagaga tcgcaagccc agagaaattg ccgcgccccg
    19741 tgatgctcat agccagtgtg ctgggccgca tgggactcat gaacggttac tggaagaaac
    19801 agctgaaaaa gaacggcgct tacgaaaaac gtcacgcgag accgtatgac ccgactcatc
    19861 tacctcgata aacaagaggg actaatttga aagagcggga agagagagaa agagaaagag
    19921 aaagagagag aaggagagag aggagagacg tcttctcgtg agcggagcga actctcgatg
    19981 ttctttctct cgtgtttttc tctctctttc tcttctcgtg agcggagcga actctcgatg
    20041 ctcttcctcg actctcacaa acaacccaca acgcaccacc caccacggct cttaaaggtt
    20101 tatttctcta tcagttcgac tccggcgctc ttgaattcat ccagcgtcct ctgaacgtct
    20161 ccttcgttgg cttcaacacc cttcgtcccg tttattatta cctttatctt gaagccggca
    20221 gaaagcgcat cgtgaaccgt gtatttcagg cagtagtcga gagcgagtcc gcagatatcg
    20281 agctcctcaa tgcctagagc cttcagcaat ccggccgttc ccagctccgt tccgtcgttt
    20341 tctctgaaga tcgagtagct gtcaactcct ggatgggtcc cctttctcac tatatagtca
    20401 aaccggttgc tttcgatatc cgggtgaaac tcggctccgt gggttccctg catacagtgg
    20461 aacggccaca gaacgggtcc gatgcccttc ttgttgtcga acggcgtgaa aggctcttta
    20521 ccgtgattcg ccgcgaaact catgtgattg tatggatgcc agtcctgacc tgcaagtatc
    20581 gtatacacgg gatctctcat ggctctattt atcacttcgt ttatccggct ggctcccttc
    20641 accgggagag aaccggtctc gtagaagtcg ttttgaacat cgacaaccag cagcgctt
//
LOCUS       DAOOLY010000010        18818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3007_length_18818_cov_5.323189, whole genome shotgun sequence.
ACCESSION   DAOOLY010000010 DAOOLY010000000
VERSION     DAOOLY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 18818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18818
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3007_length_18818_cov_5.323189"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1119
                     /locus_tag="PLD15_01075"
     CDS             214..1119
                     /locus_tag="PLD15_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB62517.1"
                     /translation="MRVKSFAIAAFLFFVALAFCQTASPQFVAIERAVSFENDGQTLC
                     GILTLPETGDRPFPVVLLLHGFLGHMNDLEVGGTGGSMYEMTAEHLAGAGLASLRFDF
                     RGSGISEGSWEETTFSGQISDTIAAISFLESLPEIDKRKIAVLGLSQGGLVAACVAAR
                     DVRVRSVVLWSAVAIPVYTYSALLGADAVGSSIKAAPGEPILASISWGGTTVLKKEFF
                     DELFTVDPVAEVARYDGPLMVVSGLTDAIVFPQPQSGRIFIDYHEGPERLFLQNSGHI
                     YDLFEGKDHLEEVIDESIQWLLETL"
     gene            complement(1172..2065)
                     /locus_tag="PLD15_01080"
     CDS             complement(1172..2065)
                     /locus_tag="PLD15_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPB62518.1"
                     /translation="MRTLKESTMREDYIDCVNSVIDYIEENLFRSITLEDLCSEIFYS
                     PVHLQRIFYYTVGETIGRYIKGRRLSEAAERLAGTSMSILEIALRCGYDSQESFTRAF
                     KSRYYVTPGKYRRLGLHFTLTQKKRLTKKKIERPMGGVNVEPKIVKRKAFKVVGLKYY
                     GNDPKNNCPKLWQEFMKRIGEIRNALPVMESYGLMCTGEEDFVDGKFDYIASVAVSDL
                     DNIPEGMVGAEIPEATYAVFTHKGRLDTLQETYEYIYGNWFQNSEYEPIGLNEFELYD
                     DRFTGEESSQFDIYIPLKKKA"
     gene            complement(2311..3252)
                     /locus_tag="PLD15_01085"
     CDS             complement(2311..3252)
                     /locus_tag="PLD15_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HPB62519.1"
                     /translation="MIFTLTLNPALDRYLYTGKLVADDTVRVDKIKDYPAGKGVDVSR
                     VINELGGHSVAIAFLGGRIGDEIEEMLNDEGVVYASVRTDGETRMNILIESDGGQYRL
                     SLPGPGLSEREIDKLHKTLDILLREGDTLLMCGSVPGGIGKDIYRVLCERMTGRKIKV
                     YIDSDKEPLAEGVKASPTGIKPNTHELDRLLGRESGGDYEEALREVSERYSIPEVLLT
                     MGKEGAMAMIEGRIYRVEVPVVPVKSAVGAGDSFLGAYCMYREMGGTVETALKMAGAA
                     SAATVMTPGTELCRREDVVKIMEKVKVNKKAQIRDWR"
     gene            complement(3274..3960)
                     /locus_tag="PLD15_01090"
     CDS             complement(3274..3960)
                     /locus_tag="PLD15_01090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPB62520.1"
                     /translation="MPNAFFVEVSGDTVRLDGIEANHLRVVRAKKGDRLTGTDGNGGI
                     YKFVLEEVQKHEATGTVIEKEFVERDGRTIIIAVAATKWPRLRILLEKATELGVDRIE
                     LFQSRRSVALLDGEKLSRYTAVVREAAKQSVNPYLPAVELQRNLDLSGAHNLLLDFQG
                     KPLRELSKELGARRKIRLIVGPEGGFTEEEISGISAASTKVSLGRRTLRVETAVIVAL
                     GLLNDYTERI"
     gene            complement(3962..5230)
                     /gene="serS"
                     /locus_tag="PLD15_01095"
     CDS             complement(3962..5230)
                     /gene="serS"
                     /locus_tag="PLD15_01095"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPB62521.1"
                     /translation="MVDIRLIRENPELVRKALENRQMGQELLEEILQLDAQRREALQI
                     VEQKKAERNSISSEIARAKASKDNSRAQSLMDRAKEISSEVKELDVRTSQIEEELRQK
                     MLYLPNIPSEKTPVGRSESDNVVVRTWGEPGKPAFEVKPHWDFGPETGLIDFERAAKL
                     SGARFVILRKDLARLERAIMNFMLDLHHSKGYEEVALPFMVKRETMLATGQLPKFEEE
                     AYRIEPDDMFMIPTAEVPLVAQHGEEIIDGELPRKYTAYSACFRREAGSYGKDVRGMI
                     RVHQFDKVELVWFTHPDFSYDALEQLTADAEDVLRKLKLPYRVVALCTGDIGFASAKT
                     YDLEVWLPSYNTYREISSCSNVEDFQARRANIRFRDIDNKLKFVHCLNGSGLAVGRTL
                     VAIIENYQREDGRIDVPEVLVPYMGQEVIG"
     gene            complement(5251..5916)
                     /locus_tag="PLD15_01100"
     CDS             complement(5251..5916)
                     /locus_tag="PLD15_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA family protein"
                     /protein_id="HPB62522.1"
                     /translation="MRRKLPIFLIVALVFAVILIAQSTVRIKADELLATERQEVFTFL
                     GNVMINKDGDIYVETPLATVTKGATDWQSFITGGETFVIFQTGEATALSLDYNLDNST
                     GLMKDVPVATIYSKEEGEKDIYIYKTDILKFDQKNEYYEGFAKETEDATPELIFIDYK
                     GDLKVDTLYFEYHGDTGMLNLKGNVFVNDEKNSRKIWASELLYNTEDDSFKGLNVEIE
                     LVF"
     gene            complement(5936..6394)
                     /locus_tag="PLD15_01105"
     CDS             complement(5936..6394)
                     /locus_tag="PLD15_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB62523.1"
                     /translation="MSRGEVRKEDAATLEKNLRIISTRIRREGRKVLRDFPITPAQFD
                     VLQTLFFQGEKRMSDISRNLGITKSTTTGLVKRLIDAGFIERRRSERDRRSFIIGISS
                     SGKSLIEKVIDRRVEYLRSVMTEISTEQVVSLEQIVSLLLEVMDSKKTAE"
     gene            6787..7488
                     /locus_tag="PLD15_01110"
     CDS             6787..7488
                     /locus_tag="PLD15_01110"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPB62524.1"
                     /translation="MRSSEDYEALERFDSTFIERFGTVAGLDEAGRGPLAGPVVAAAV
                     ILRGPVRGVNDSKLLSAKDRESLYGEIADRAYVGIGLSTPEEIDLLNILNATKLAMNR
                     ALDGLSVRPSYVIIDGKYLNLKIRGRCIVGGDRLSASIASASVMAKVFRDRLMNDLGN
                     LFPEYGYERHKGYGTEKHLKAITVYGPSMWHRLTYKPVRESARPEMVSRWLRDSAVNE
                     DRLFRAGLLTPEVLK"
     gene            7485..8270
                     /gene="mazG"
                     /locus_tag="PLD15_01115"
     CDS             7485..8270
                     /gene="mazG"
                     /locus_tag="PLD15_01115"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPB62525.1"
                     /translation="MNNGELKENWLKLIETMKRLRSPGGCEWDIAQSHESLKPYMIEE
                     AYEVLEAIDRRDDAELVEELGDVMLQVIFHAQVAAERDAFDINDVIKVLTDKLVRRHP
                     HVFSSSPGYSYRQWEKIKASEKGEAPGQKSSIGKINRALPALSLARRVQENASAVGFD
                     WTKIDGPREKLDEEIEEVDQAVKSGDKKKIEEEIGDLLFTVVNFSRFLDVDPESALRK
                     STEKFVNRFNEMESHIEKYGLEIEGMTIDELNHLWEKAKEGTN"
     gene            8267..9295
                     /locus_tag="PLD15_01120"
     CDS             8267..9295
                     /locus_tag="PLD15_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPB62526.1"
                     /translation="MKRLLIAVLFILSAVIPVLAQTENASDDTVAATVNGEIIYMDTF
                     LSKVLPSYTEISKRIEEVDPVFSDLLLNTQAGQNLLAEYEKRSLETLIEETLVMQYAQ
                     SVGIEIDIKAIRETVNKSIMDTLTELNIAKSDADLFYILKGYIDGLASYESKVVRDLA
                     YREVLNTLYLAVTENATLSEDEVIQYYQANSSRYAVEEEKVTIKLLLFDSFSEAYSVW
                     KVASRDSNPAAVLDTFPDVQSKSYTRQEIESVNPELVRSLFKATKGELLPSVVTMDNR
                     YAVIYLVSYTPAGTKSVDEMRSEIEETLINEKRDLLWKSWREKEFEPFKQNSTITRYY
                     DNSEGEQS"
     gene            9292..9591
                     /locus_tag="PLD15_01125"
     CDS             9292..9591
                     /locus_tag="PLD15_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HPB62527.1"
                     /translation="MKITKEKVMEALSQVYDLEIGFDIVSLGLIYGVEVEDSNVKVKM
                     TMTTPMCPLAGLMLEDARRKVSEIEGINDVKMELTFDPPWTPEMASDDVRKILGL"
     gene            9588..10448
                     /gene="prmC"
                     /locus_tag="PLD15_01130"
     CDS             9588..10448
                     /gene="prmC"
                     /locus_tag="PLD15_01130"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPB62528.1"
                     /translation="MKAKELLKNCIERLDRAGIDCSRSVVRLLFKELLYLSDSEIILG
                     TVEVPHRLCLSMNGAIERLIEGEPLDYVVGRRNFLGVWLKLNNSVLIPRPETEELVQM
                     VIDRERDSTVFADVATGSGAIACALASHLSHSRVFASDLSLAALEVAMENAAKNGLSN
                     IRFYHGDNLAALEDFLEEIEVIVSNPPYIKTSMLKSLDRKVAGYEPLLALDGGSDGLD
                     FYREFFKHLPEGKRVYLEIAEYSVKGLSELVAPLKNYEYSFEKDFYGSYRFMFLHPEG
                     RESRRRTPRE"
     gene            complement(10393..11148)
                     /locus_tag="PLD15_01135"
     CDS             complement(10393..11148)
                     /locus_tag="PLD15_01135"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPB62529.1"
                     /translation="MRNTTDEAAFFLKLLRNSHKTVVLTGAGVSVASGIPDFRSPDGL
                     YSKVSPEVFELDFFLDNPAEYYKIAQERIHTMADRKPNPTHFLLSRLQREGMIGEIIT
                     QNIDGLQQRAGAKDVIELHGSVSSFSCMKCHRSYSKDELEKLFEESEVPWCNCSGLIK
                     PDIVFFGEALPIEAINRAERAAIDSELFVAMGSSLVVYPAAQFPVIAKSSGAILAIVN
                     RDSTGLDYMADNIFNVTLEEFSGEILALLDGGT"
     gene            complement(11145..11732)
                     /locus_tag="PLD15_01140"
     CDS             complement(11145..11732)
                     /locus_tag="PLD15_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4897 domain-containing protein"
                     /protein_id="HPB62530.1"
                     /translation="MKFNTLLIILVVVMVGMTAVNMFMAFNNRLDIETLSSSSNYTYD
                     YEGRATLDIETEILFNKPNQMTQFLEQYEKPKEQQQADFQESITKFAESFKRIMTVED
                     FQSTATVLGSNRVRVVEHAVIAGFSAVEEGIVNTDMGDMEFDLTGVESSLTLTIPVGA
                     KVVSVEPEPSSTIGGNIFIWSNTGKTPFPKVRFGQ"
     gene            11880..12863
                     /gene="trpS"
                     /locus_tag="PLD15_01145"
     CDS             11880..12863
                     /gene="trpS"
                     /locus_tag="PLD15_01145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB62531.1"
                     /translation="MRILSGMRSTGRLHLGHFVTLEKWKELQDSGNDCFYFVANWHGL
                     TSHIDVAENFKEWTLDIIRTYVAVGLDPEKSVLFIQSAIKEHAELFVYFSMLIPVSRL
                     ERVPTYKDQKEQILDRDLSSGGFLLYPVLQAADILMYLANGVPVGEDQIYHVELTREV
                     ARKFNNQFGEVFPEPEPILAKVPKLPGTDGRKMSKSYNNFILIDTDEKELWKMLAPMM
                     TDPARKRRTDPGDPEKCPVWDYHKAFTTSQSEKDWVVNGCKTAGIGCLDCKKLLQKNL
                     VTSLSPVWEKLAYLKDNPQVVDRIIEEGNSRARAIAQETMRKVLQATKLGW"
     gene            12866..13564
                     /locus_tag="PLD15_01150"
     CDS             12866..13564
                     /locus_tag="PLD15_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB62532.1"
                     /translation="MEQLQLVFNLPEFEGPLDLLLYLAKKHRVDIRSIPITFIADEFI
                     AHINKMKSLDMDIASDFLVMASTLMEMKSKALLPRLSDKEAEVFENQQKELFRRVEEY
                     QTVKELSKRFRIKLYDSYSYERAMAKPAVEADRGDELPDKLMIAFKSILKETALRDRV
                     YTIVAESVRLEDRMQQIEGLYDRIELYEFLRELESRSEIIVSFLAILELLKLNRYILE
                     SLEPLVIRRRSHER"
     gene            13554..14165
                     /gene="scpB"
                     /locus_tag="PLD15_01155"
     CDS             13554..14165
                     /gene="scpB"
                     /locus_tag="PLD15_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB62533.1"
                     /translation="MNDELSRKAVIEALIFSARNGIEPRKIASIVGMKLNQVTLLLDE
                     IESDYATSEHGVMIKSINGKLRFYTKPELQNYVSQISTRPIVSITDTQMEALAIVAIK
                     GPLTKNDVELIRGRSTQNQLLELSKMGLIGKRKSKLPGRPYLYRITKKFYDLFQLEDL
                     SEIVQGLSLGGGEEIETAGDTVPADGSVQEKIDTGDTGRESQD"
     gene            14077..14820
                     /locus_tag="PLD15_01160"
     CDS             14077..14820
                     /locus_tag="PLD15_01160"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB62534.1"
                     /translation="MRLQEILSRLTGLSRRKSTQAILDGRVRIDGEVERYPRRELDPQ
                     SSSIEFDGKSLEFKKQATVVYLINKPAGYLSAMTDDRGRKTLSDLIAGKIEERVFHVG
                     RLDQGTSGLLILTNDGDLAHLVSHPASEIEKTYLVKIRGKLDDAQLQTVRDGITLEDG
                     FTTSPARIRLVRSDRETTTLRLILHEGHKREIREMFKALGKSVVSLVRVSIGGLDISL
                     VPRPGEIKKLGKKEIELIKMGSKRKSQER"
     gene            complement(14849..15154)
                     /locus_tag="PLD15_01165"
     CDS             complement(14849..15154)
                     /locus_tag="PLD15_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPB62535.1"
                     /translation="MSDVCAIIVIIERGRAEEIVKAARAAGARGATVSFGRGTSDNEI
                     RKFLHINVESSKEIILILTKKSDLDGIFQAVVDSGKLEEDGKGIAFVLDVPKMVGRL"
     gene            complement(15166..15870)
                     /locus_tag="PLD15_01170"
     CDS             complement(15166..15870)
                     /locus_tag="PLD15_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPB62536.1"
                     /translation="MKVAELIKNLWMIVRSVIPLAGFLIIFQAVVFRKPLGDLKSLFL
                     GIVLSVFGLFLFLKGASISLIPLGSSFGRSIVALDNVLLIIVIAFMLGFLATLVEPAL
                     TSTSLEIEEVSTGTIPSASITYSAAVGFGLGMALGVFKIVSGTSIKTIMVPFLVAMTV
                     LVILSSKEMVGIAFDVAAATTGPVNIPITVAISIGLSSVIQGSDPLLNGFGLVGFAFM
                     GAAMSILFLGTVMRFF"
     gene            16064..16900
                     /locus_tag="PLD15_01175"
     CDS             16064..16900
                     /locus_tag="PLD15_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB62537.1"
                     /translation="MIYRQLKNSGVKIAAIGLGTWAIGGRESPDSSRDGEYVDTIARA
                     IEMGYNHIDTAEYYAGGHTEELIGEAITLFDRRELFLVSKVWPTHLREKDLHKALDGT
                     LKRLRTDYVDMYLVHWPNPEVPLRETMKAMADEVRAGRTRFIGVSNFDTNLLKKAVAV
                     STEPVVNNQVLFNIEDRQAMYELLPFCQSEGITLTAYSPLKRNLVSEFTGQALERLAE
                     KYYASPQQIMLAWLLGKEGVIVIPKAEKTDHLKSNIDSKNIVLTPEDMKMLDGLEGGA
                     LV"
     gene            16897..17550
                     /locus_tag="PLD15_01180"
     CDS             16897..17550
                     /locus_tag="PLD15_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488063.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB62538.1"
                     /translation="MKALYEEFKKALDEQNKDRAVEICIKAIDEEKTDIPGLYLHILT
                     PALNEWDCDYEVDRLCIWNEHVRSSIVRTVMECCYPYIARKRRERSSDGRSPKVAVVC
                     PVEEYHEIGARMVADIFALAGYDVTFAGANTPQDSFLEAMKVLRPLYVAVSISNYYNL
                     ISGRCVISQIKQWTPQSRIIVGGNAFKNNPEYWKEIGADIYLTDPVEILKMGERDLK"
     gene            17547..18713
                     /locus_tag="PLD15_01185"
     CDS             17547..18713
                     /locus_tag="PLD15_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62539.1"
                     /translation="MKLALSIAFRFISSSKAQTLLIILGIAIGVSVQVFIGSLIQGLQ
                     KDLVNTTIGNSSQVTITSNTSDRKIDNWQKILEEVESIQLPTPLTALSASADVPVFIS
                     SGDRTISVLLRGLQFPQANSIYGIDAKIIEGELPKTAGEVLVGKDLKDELGLQKGGQI
                     TIITPDRSIETLRIAGVFDLKVASLNKSWLISSISTSQGVGKLGDSVTSIEMQVEEVF
                     KADEDATAIASALADAKLDVTNWKAQNADLLTGLNGQSISSIMIQVFVIIAVSLGIAS
                     VLAITVVQKSRQIGILKAMGLKDSVSSQVFLFQGLSLGIIGAVVGIAFGFLLILMFST
                     FAVGPDGEPVVGIYVNYGFIMLSAFIAIGASTIAALIPARRSSKLSPVEVIRNG"
BASE COUNT         4554 a         4781 c         4909 g         4574 t
ORIGIN      
        1 ctcaattata cgatcacaaa tccgaaatac ttaatctaga gactaatata tatgacataa
       61 cagcagaatc tttacttgct ggagaacctc aagggctcgt tagccgtttg ttttttaggg
      121 ttatgttcca agcgaacatc aaattgactt cggagtattc acgaatcaat atatctctca
      181 tccctgttat actgaactgg agggggtgtc tttgtgagag ttaagagttt tgcgatagcg
      241 gcatttttgt ttttcgttgc tctggccttc tgtcagacag ccagtcctca gttcgtcgca
      301 atcgagagag ccgtctcgtt cgagaatgac ggtcaaacgc tgtgcgggat tctcactttg
      361 ccggagaccg gcgacaggcc ctttccggtg gtgctcctgc tccacggttt cctcggtcac
      421 atgaacgacc tcgaggtggg gggaacgggc ggttcgatgt acgaaatgac cgccgaacat
      481 ttggccgggg ccgggctcgc ttctctcagg ttcgatttca ggggttcggg tataagcgag
      541 ggaagctggg aagagacgac tttctccggt cagataagcg atacgatcgc cgcgatttcc
      601 tttcttgaga gccttccgga gatcgataag cgaaagattg ccgttctggg cctcagtcag
      661 ggcggtctgg ttgcggcctg tgttgcggca agggacgttc gggtgaggag tgtcgttctc
      721 tggtcggccg ttgctattcc cgtttacaca tattcggcct tgctgggcgc cgatgcggtg
      781 ggctcgtcga taaaagccgc gccgggtgag ccgatcctgg ccagtatctc gtggggaggg
      841 acgacggtgt tgaaaaagga gttcttcgac gagcttttta ccgtagaccc ggtagccgag
      901 gtggcacgct acgacggccc gttgatggtg gtctcgggtc tcaccgacgc catagtcttt
      961 cctcagccgc aatcgggaag gatattcatc gattaccacg aaggaccgga gaggctgttc
     1021 cttcaaaact ccgggcatat ctacgatctg ttcgagggta aagaccatct cgaagaggtt
     1081 atagacgaaa gtattcagtg gctactggag accttataaa aatgggggat cttataaata
     1141 ttataagatc cccgtatagc gttatgagtc gctacgcctt ctttttcaag gggatgtata
     1201 tatcgaactg ggagctttct tcaccggtga acctgtcgtc gtagagttcg aactcgttca
     1261 atccgatggg ttcatactcc gagttctgga accagttccc gtagatgtac tcataggtct
     1321 cctgcagggt gtcgagcctg cccttatggg tgaacacggc gtaagtggct tccggtattt
     1381 cggctcccac cattccctcg ggtatgttgt caaggtccga gacggccact gaggcgatat
     1441 agtcgaactt gccgtccacg aagtcctcct ctccggtaca catcaggccg tagctttcca
     1501 taaccggtag ggcgttcctg atttctccta tccttttcat gaattcctgc cagagctttg
     1561 ggcagttgtt cttgggatcg tttccgtagt acttcagccc cacaactttg aaggcctttc
     1621 gttttacgat tttcggttcc acgtttaccc ctcccatcgg tctttcgatc tttttctttg
     1681 tgagccgttt cttctgagtg agtgtgaaat gcagtcccag gcgcctgtat ttaccgggtg
     1741 tgacgtagta tctgctcttg aaagctctcg tgaaggattc ctgcgaatcg taaccgcacc
     1801 ggagggcgat ctccagtatc gacatcgatg taccggccag tctttcggcc gcctcgctca
     1861 atcttcttcc ctttatgtac cggcctatcg tctcaccgac ggtgtagtag aagatgcgtt
     1921 gaagatggac cggtgaatag aagatctcag agcagagatc ttccagggtt atcgatcgga
     1981 aaaggttctc ctcaatatag tcgattaccg agttcacgca gtcgatgtag tcttctctca
     2041 tggtagattc ctttagcgtc ctcaaccctt cccacctcca cagccatggc agaaataatt
     2101 atacggggaa agtctgaggg ttttttgatc gaaagtatca cgatggaagt caactgtaaa
     2161 ttgcctgccg tgcttggggt ctccggttaa aggctctcac cgacgacgat cccggcaaaa
     2221 gtctgccgga aaagttcgaa gagatcaata gaccacgatt ctctcgatga ccctggccga
     2281 ttcgacggta tttctcattt ccttcgatct ctatctccaa tccctgatct gggccttttt
     2341 gttcacttta accttctcca tgatttttac gacgtcctcg cgccggcaca gctcggtgcc
     2401 cggtgtcatg accgttgcgg cgctggccgc tcccgccatt ttgagggccg tttccacggt
     2461 gccgcccatc tccctgtaca tgcagtaagc cccgaggaag gaatctcccg cgccgaccgc
     2521 gcttttgacg ggtactaccg gcacttcgac tctgtagatt ctcccctcga tcatggccat
     2581 agccccttcc tttcccatgg tgaggagaac ttcgggtatg gagtatctct ccgaaacctc
     2641 tctcaaagcc tcttcgtaat cgcctccact ttcccttccc agcagcctgt cgagttcgtg
     2701 agtgttcggc tttatacctg ttggcgaggc tttgacgcct tcggccagag gctctttgtc
     2761 ggaatctatg tagactttga tctttctccc ggtcatcctc tcgcagagaa ccctgtaaat
     2821 atccttgccg atacccccgg gaacgctgcc gcacatgagc agagtgtcgc cctctcttag
     2881 aagtatgtcg agggtcttgt ggagtttgtc tatctctctt tcgctcagac ccggtccagg
     2941 aaggcttaat ctgtactgcc cgccgtcgct ttcgataagt atgttcattc tggtttcgcc
     3001 gtcggtcctg accgaggcgt aaacgacgcc ttcgtcgttg agcatctcct cgatctcgtc
     3061 gcctatgcgc ccccctagaa aggcgatagc caccgagtgg ccgcccagct cgtttatgac
     3121 ccgggagaca tcgacacctt tgccggcagg gtaatctttg attttatcga ccctgacggt
     3181 atcgtcggcg accagtttgc ccgtgtagag atatctgtcc agagccgggt tcaaagtcag
     3241 tgtgaaaatc atcttgcttg cgccaccttt cctctatatt ctttcggtgt agtcgttcaa
     3301 tagaccgagt gccactatca cggccgtttc caccctcagt gttctcctgc ccagcgagac
     3361 tttcgtgctg gcggcggaga tccccgatat ctcttcttct gtgaaaccgc cctcggggcc
     3421 gacgatcagt cttatctttc ttctggcgcc gagctctttc gagagttcgc ggagcggctt
     3481 gccctgaaag tccaggagca gattgtgagc tcccgacaga tcgagatttc tttgcagctc
     3541 caccgccggc aggtatgggt tcacgctctg cttggcggct tctctcacga cggccgtgta
     3601 tctggagagt ttttcaccat ccagaagcgc caccgagcgc cggctctgga agagttctat
     3661 gcgatctacc ccgagttcgg tcgccttctc cagtaaaatg cgaagccgcg gccacttcgt
     3721 tgcggccacg gctattatta tcgttctgcc atctctttcc acgaattcct tttcgattac
     3781 cgtgccggtg gcttcgtgct tttggacttc ctctagcacg aatttgtaga tgccgccgtt
     3841 gccgtccgtc cctgtgagtc tatctccctt tttcgcccgt accactctca ggtgattggc
     3901 ttcgataccg tcgagcctta cggtatcgcc cgagacttcc acgaagaagg cgttgggcaa
     3961 actatccgat tacctcctgg cccatgtagg gaacgagaac ttcgggcacg tctatcctgc
     4021 cgtcttctct ctggtagttt tcgattatcg ccacgagcgt tcttcccacg gccagtccgg
     4081 agccgttgag acagtgaacg aacttgagtt tgttatcgat gtccctgaac cttatgttgg
     4141 ccctcctcgc ctggaagtct tcgacgttcg agcacgagga gatctcccta taggtgttgt
     4201 aggaaggcag ccagacctcg aggtcgtagg ttttggccga ggcgaaccct atgtctccgg
     4261 tgcagagcgc caccactctg taaggcagct tcaatttgcg caacacgtct tcggcgtcgg
     4321 ccgtgagttg ttcgagagca tcgtaggaga agtcgggatg ggtaaaccag acgagctcca
     4381 ccttgtcgaa ctggtggacc cttatcatgc cgcgaacgtc cttgccgtaa gacccggctt
     4441 ctctcctgaa gcaggcgctg taggccgtgt attttctggg aagctcaccg tctattatct
     4501 cctcgccgtg ctgtgcgacc agcggtacct cagcggtcgg tatcatgaac atatcgtccg
     4561 gctctattct gtaagcttcc tcttcgaatt tcgggagctg cccggtcgcc agcatcgtct
     4621 ctctcttgac catgaagggg agggcaacct cttcgtaccc cttgctgtgg tgcaggtcga
     4681 gcatgaagtt cattattgcc ctttccaggc gtgccagatc ctttctcagt ataacgaagc
     4741 gcgccccgga gagtttcgcg gcccgctcga agtctatcag ccctgtctcc gggccaaagt
     4801 cccagtgcgg tttgacttcg aaggccggtt tccccggttc accccaggtc ctcaccacaa
     4861 cgttgtcgct ttcgctcctg ccgacagggg ttttctccga tgggatgttc ggcagataca
     4921 gcatcttctg gcgcagctct tcttctatct gggaggttct aacgtccagt tccttgactt
     4981 cggaggagat ttccttcgct ctatccatga gagactgcgc tctggaattg tctttgctgg
     5041 ccttggcgcg ggctatctcg gaagagatgc tgtttctttc ggccttcttc tgctcgacta
     5101 tttggagcgc ttctcttctc tgcgcatcga gctgaagtat ctcttcaagt agctcctgtc
     5161 ccatctgtcg attttctaga gcctttctta ccagttcagg attttcacgg atcagcctga
     5221 tatctaccat aattctcctc ctgcacctcg ctagaatacg agttctatct cgacgttcaa
     5281 tcctttgaag gaatcgtctt ccgtattgta caacaattcg gaggcccaga tcttcctgct
     5341 gtttttctcg tcgttcacga aaacattacc cttcaggttg agcattcccg tgtctccgtg
     5401 gtattcgaag tagagcgtat cgactttgag atcgcccttg tagtctatga atattagttc
     5461 cggagtcgcg tcttccgtct ccttggcgaa accttcgtaa tactcgtttt tctggtcgaa
     5521 cttgaggata tccgttttat atatgtagat atccttttcg ccttcttcct tggaatagat
     5581 cgtcgcaacg ggtacgtcct tcatcaggcc ggtcgaattg tccaggttgt agtcgagcga
     5641 cagcgcagtt gcctcgccgg tctggaagat aacgaacgtt tcgcccccgg ttatgaacga
     5701 ctgccagtcg gtcgcgccct tagtgactgt tgccaggggc gtctcgacgt agatgtcgcc
     5761 gtccttgttt atcatgacgt ttcccaaaaa cgtgaagact tcctgtcttt ccgtcgcgag
     5821 aagctcgtcg gcctttatgc gcactgttga ctgagctatg agaatcactg caaacacgag
     5881 cgcaacaatc aaaaagattg gtagttttct tctcatcttt acctccggta aacggttatt
     5941 ctgccgtttt tttactgtcc ataacttcga gaagaaggga gactatttgc tcgagggata
     6001 caacctgttc ggtacttatc tctgtcatca cggaacgaag atattccact cttctgtcga
     6061 taaccttttc gatgagggat tttcccgaac tggaaatacc aataatgaag gatcttcgat
     6121 ctctttcaga gcgtcttctt tcgatgaagc cggcatctat gagtcttttc acgaggccgg
     6181 tagtggtact cttggttatc cccagattgc gacttatatc gctcatcctc ttttcgccct
     6241 gaaagaaaag cgtctggaga acatcgaatt gtgcgggagt tatcggaaag tccctcaaaa
     6301 cctttcgacc ttcccttctt atcctcgtag aaatgattcg cagattcttc tccagagttg
     6361 ctgcgtcctc ttttctgacc tcacctcggg acatctccac acctccatac tcccaagcgt
     6421 tggtttggaa tcgatacctt actgataagt tattataaca ctttcggcct gctacattat
     6481 gactgagagg gggatagaaa ccgttcaaag gaaccgtggt ggatggtgtg ttgtaggtga
     6541 gggagagatg ccggatcggg gtccggcatg atgggaagaa agaaggactg ccatccagag
     6601 ttcgcgtctc gccctctctt atctctcttc ctctctcttt ctctctcctc ttctcgctct
     6661 ttccaaggac gggtccacgc tccgggacga ctgacgaagg acggctcttc ccgctcttcc
     6721 cacccctgtt atcgacggta aaatccggtt cgtgaaggca atcgaagtct gatgtataat
     6781 cgccttatga gatcttcaga agactatgaa gcgcttgaac gttttgattc gacattcata
     6841 gagaggtttg gaaccgtcgc cgggctggat gaggccggca ggggtccgct ggccggtcca
     6901 gtggttgccg ccgcggtgat attgcgcggt ccggtgaggg gagtcaacga ttccaagctt
     6961 ctttccgcga aggacaggga atcgctttac ggggagatag ctgatcgcgc atatgttggg
     7021 atcggtctct cgacaccgga ggagatagat cttctgaaca ttcttaacgc cacgaaactg
     7081 gccatgaacc gtgctcttga cggacttagc gtgaggccct cttacgtgat tatcgacggg
     7141 aaatacctga atttgaagat aaggggaagg tgcatcgtgg ggggcgacag gttgagcgcc
     7201 tcgatcgcca gcgcgtcggt gatggctaaa gttttcaggg acagactgat gaacgatctg
     7261 gggaacctct ttcccgagta cggttatgaa cggcacaaag gatacggaac cgagaaacac
     7321 ctgaaggcca tcacagttta cggcccaagt atgtggcata gattgacgta caaaccggta
     7381 agggaaagtg caagaccgga aatggtttcg agatggttga gagatagcgc cgtgaacgaa
     7441 gaccggctgt tcagggctgg attgcttact ccggaggtac tgaagtgaat aatggagaat
     7501 tgaaggaaaa ctggttgaaa ttgatagaga cgatgaagag gctccgttca ccgggaggct
     7561 gtgagtggga tatagcccag tctcacgaaa gcctaaaacc gtacatgata gaagaggctt
     7621 acgaagttct ggaagcgatc gacaggcggg atgatgccga actggtcgaa gagctcggag
     7681 atgtcatgtt acaggtgatc tttcacgcgc aggtggcggc cgaaagggat gctttcgata
     7741 taaacgatgt gataaaggtt ctgaccgaca agctcgtgag aagacatcca cacgtcttct
     7801 ctagctcccc cggttactcc taccgccagt gggagaagat caaagcgtct gaaaagggtg
     7861 aagcgccggg acagaaatct tcgatcggaa agatcaacag ggctcttccg gcgctcagcc
     7921 tcgcgcgtcg ggtacaggag aacgcctcgg ccgttgggtt tgactggacc aagatagacg
     7981 gtccacggga aaagctcgac gaagagatag aggaagtcga tcaagccgtg aagtctggcg
     8041 acaagaaaaa gatagaggaa gagatcggag atctgctctt cacggtcgtt aacttctccc
     8101 gctttctcga tgtggatccc gaatcggcct tgagaaaatc taccgaaaaa ttcgtgaacc
     8161 gtttcaatga gatggagagt catattgaga agtacggttt agaaatagaa ggcatgacca
     8221 tagatgagct gaatcatctg tgggaaaaag cgaaggaggg tacaaattga agaggctgct
     8281 tatcgctgtc ctgtttatac tttctgctgt tatccccgtt cttgcgcaga ccgagaatgc
     8341 ttccgacgat accgtggctg ccacggttaa cggtgaaatc atttatatgg atacctttct
     8401 ttcaaaggtt ctgccgagtt atacggagat ctcgaagcgt atagaagaag tcgatccggt
     8461 cttttccgat ctacttctca acactcaggc cggtcagaat ttgctggcag aatacgagaa
     8521 gagatctctc gagactctga tcgaagagac gctggtgatg caatacgctc agtcggttgg
     8581 aatcgagata gatattaagg caataaggga aacggtcaac aaatccatca tggatacgct
     8641 cacagagctg aatatagcaa aatccgacgc tgatcttttc tatatactca agggctacat
     8701 agacggtctt gcgtcctacg agtcgaaggt cgtcagagat ctggcctaca gagaagtcct
     8761 gaacaccctt tatctagccg ttacggaaaa cgcgacattg agcgaggatg aagtcattca
     8821 gtactaccag gcaaattcct ccagatacgc ggtggaggaa gagaaagtta ccattaaact
     8881 cctcctgttc gattccttct cggaagctta ttccgtctgg aaagtagctt ccagggattc
     8941 taatccggcc gcggtactgg acaccttccc cgacgttcag agtaagagtt atacaaggca
     9001 ggagatcgaa agcgtcaacc ccgagcttgt gagatcgcta ttcaaagcca ccaagggtga
     9061 gttacttcct tcggttgtga cgatggacaa cagatacgcg gttatctacc tggtttccta
     9121 tacaccggcc ggcaccaaaa gtgtggatga aatgcgcagt gagatagaag aaacccttat
     9181 caacgagaag agggaccttc tctggaagag ctggagagaa aaagaattcg aaccgttcaa
     9241 gcagaattca acaattacga gatattacga taacagcgaa ggagaacagt cttgaaaata
     9301 actaaagaga aagtgatgga ggccctgagt caggtttacg acctggagat aggtttcgat
     9361 atcgtctcgc tcggcctcat atacggagtg gaagtagaag acagcaacgt gaaggtgaag
     9421 atgacaatga ccacacccat gtgccctctg gcaggcttga tgcttgaaga cgccaggaga
     9481 aaggtcagtg agatcgaagg aataaatgac gtgaagatgg aactaacttt cgacccaccc
     9541 tggactcccg agatggctag cgatgatgtg aggaagattc tggggctttg aaagcgaaag
     9601 aacttctaaa aaactgcata gagagactcg acagagcggg gatcgactgt tcccgctctg
     9661 ttgttcgtct cctcttcaaa gaactcctct atttaagcga cagcgaaatt atcctcggaa
     9721 cggtggaggt accccaccga ttgtgccttt ctatgaacgg cgcgatagaa aggttaatcg
     9781 aaggggagcc tctggactat gttgttggcc ggcggaattt tctcggtgtc tggttgaagt
     9841 tgaataattc ggtcctcata cccagaccgg agaccgagga gctggtgcag atggttatcg
     9901 acagagagag agattcgacc gtcttcgccg atgtggccac cggaagcggc gccatagcct
     9961 gcgcgctggc cagccatctt tctcactctc gcgtttttgc cagcgatctg tctctggctg
    10021 cgctggaagt tgccatggaa aacgccgcca aaaacggtct gagcaatata cgcttctacc
    10081 acggggacaa tctggccgcc ctggaagact tcctggaaga gatagaggta atagtgtcca
    10141 atccgcccta tataaagact tccatgctga aatcgctcga caggaaagtg gcgggatacg
    10201 aacccctcct ggcgctcgac ggtggcagcg acgggcttga tttttaccgt gagtttttca
    10261 aacatctccc tgaagggaag cgggtctatc tggagatcgc tgagtacagc gtgaagggat
    10321 tgtccgagct ggtggctccg ctgaaaaact acgaatactc tttcgaaaag gatttttacg
    10381 ggtcttacag attcatgttc ctccatccag aagggcgaga atctcgccgg agaactcctc
    10441 gagagtgaca ttgaatatgt tatcggccat atagtccagg cctgttgaat ccctgttaac
    10501 tatcgccaga attgcgccgg aggacttggc tatcacggga aactgcgccg ctggatagac
    10561 aaccagcgaa gaacccatcg ccacgaaaag ctccgaatcg atcgcagcac gctcggccct
    10621 gttaatggcc tcgattggga gtgcctcgcc gaaaaaaacg atgtcgggct ttatcagtcc
    10681 cgaacagtta caccagggaa cttcgctctc ctcaaacaac ttttccagct cgtctttgga
    10741 ataactcctg tggcatttca tgcaagagaa agaagagacg cttccatgaa gctctataac
    10801 atctttagcg ccggcgcgtt gctgcaggcc atctatgttc tgagttatta tctctcctat
    10861 catgccttcg cgctggagcc tggaaagcag aaaatgagtg ggattgggtt ttctgtctgc
    10921 catcgtgtgt attctctctt gcgcgatttt gtagtattcg gcagggttat ccagaaagaa
    10981 atccagctcg aaaacctccg gagagacttt gctgtaaagc ccatccggac tcctgaaatc
    11041 ggggataccg ctggccacag agacaccggc gccggtcagg acgacagtct tgtgcgaatt
    11101 cctgagtagc ttcaggaaaa aggccgcttc atccgtcgta tttctcactg gccgaacctg
    11161 accttcggga agggcgtttt tccggtgttg ctccatatga agatgtttcc tcctattgtg
    11221 ctgctcggtt ccggctctac gctaacaacc ttagcaccga ccggaattgt caaagtcagc
    11281 gacgactcta cacccgtgag atcgaactcc atatcgccca tgtccgtatt tacaatgcct
    11341 tcctcaactg cagaaaagcc cgctattacg gcgtgctcga ccactcttac ccggttggaa
    11401 cccagcaccg ttgcagttga ctggaagtct tccaccgtca tgatcctctt gaaactctcg
    11461 gcgaacttgg tgatggactc ctggaaatca gcctgttgct gctctttagg cttttcgtac
    11521 tgctcgagaa actgtgtcat ctgattaggt ttgttgaaga ggatctcagt ctctatatcc
    11581 agtgtagcgc gaccttcata atcgtatgta tagttgctcg aagaggaaag cgtttcgata
    11641 tccagcctgt tgttgaaggc catgaacatg ttcacggcgg tcataccgac catgaccact
    11701 acgaggatga ttagaagggt attgaatttc atatattacc tcccgatacc tgatttatta
    11761 tagtaacccc tcaaatatga taacatcatt ctatgctaaa atacattgta agaaccttta
    11821 ccgtaccggc ttgaccggac atcgtataga gccagagttt cgggtttgga gggatttcag
    11881 tgaggattct cagtggcatg cgctctaccg gcagacttca tctgggccat tttgtaacgc
    11941 ttgaaaagtg gaaggagctt caggacagcg ggaacgattg tttctacttc gttgccaact
    12001 ggcacggatt gacttctcat atagacgtcg cggaaaattt caaggagtgg acactggata
    12061 taatcaggac ttatgtagcc gtggggctcg acccagagaa gtccgttctc ttcatacagt
    12121 cggcgataaa ggagcacgcc gagctctttg tttacttctc catgctcatt cccgtatcga
    12181 gactcgagag ggttcccacc tacaaggatc aaaaagagca gattttggac agggatcttt
    12241 cgagcggggg cttcctcctc tacccggtct tgcaggctgc cgatatactc atgtatctcg
    12301 ccaatggcgt tcccgtggga gaggatcaga tttatcacgt tgaattgacg agggaagtgg
    12361 cgcgtaaatt caacaatcag ttcggcgagg tcttccccga accggagccg atcctggcga
    12421 aggttcccaa actgcccggt accgacggtc gaaagatgtc caaaagctac aacaacttca
    12481 tactcataga taccgacgag aaggaactgt ggaaaatgct ggcccccatg atgaccgatc
    12541 cggccagaaa acgcaggacc gaccccggcg atccagaaaa atgtccggtc tgggattacc
    12601 ataaagcctt caccacctca cagagcgaga aggactgggt ggtgaatggt tgtaaaaccg
    12661 ccggaatcgg ctgccttgac tgcaagaagc tattgcagaa gaacctggtg accagcctct
    12721 cgccggtctg ggagaaactg gcctatctga aggacaaccc acaggtggtc gatagaataa
    12781 tagaggaagg caactcaagg gcccgcgcca ttgcgcagga aactatgcgc aaagtgttgc
    12841 aagcgacgaa gctggggtgg tagaagtgga gcagctgcag ctggtcttca atttaccgga
    12901 atttgaagga ccgctcgacc tgctactata tctagccaaa aaacaccggg tcgatattcg
    12961 ctcgataccg ataaccttca tagccgatga gttcatagcc cacataaaca agatgaaatc
    13021 tctggatatg gatattgctt ccgacttcct ggtgatggcc tccactttga tggagatgaa
    13081 gtccaaagcc ctgctcccca ggttgagcga caaggaggcg gaggtcttcg agaatcagca
    13141 gaaggaactc ttcaggagag tcgaagagta ccagaccgtg aaggaacttt ccaaacgttt
    13201 cagaataaag ctttacgatt cctacagcta cgagagagcc atggcgaagc ccgccgttga
    13261 agccgatagg ggcgacgagc tgcccgacaa actcatgatc gctttcaaat cgatactgaa
    13321 agaaactgct ttgagagaca gagtgtacac gatagtcgcc gaaagcgtga ggcttgaaga
    13381 caggatgcag cagatagaag ggctttacga caggatagag ctgtacgaat tcctgaggga
    13441 gctcgaaagc aggtcggaaa taatagtttc ctttctcgcc atccttgagc ttctaaaact
    13501 caacagatac atactcgaga gcctggaacc tctggtaatc aggaggcgtt cacatgaacg
    13561 atgaactctc cagaaaagcc gtgatagagg ctctcatatt ctcggccagg aacggtatag
    13621 agcccaggaa gatcgcctcg atcgtgggga tgaagctaaa ccaggtaaca ctccttctgg
    13681 acgaaatcga gagcgattac gccacttccg aacacggagt gatgataaaa tccatcaacg
    13741 gcaagttgag gttctacacc aaacccgagc tgcaaaacta cgtgagccag atatccacca
    13801 gaccgatagt cagtataacc gacacccaga tggaggccct cgcgatcgtc gcgataaagg
    13861 ggcccctcac taagaacgac gtggaactaa tcaggggccg ttctacacag aaccagctcc
    13921 tagagttatc taagatgggg ttgatcggca agaggaaatc caagctgccg ggccgcccgt
    13981 atctttacag aataacgaag aaattctacg atctcttcca gctggaagat ctttcggaga
    14041 ttgtccaggg cctttccctg ggcggaggag aggaaattga gactgcagga gatactgtcc
    14101 cggctgacgg gtctgtccag gagaaaatcg acacaggcga tactggacgg gagagtcagg
    14161 attgacgggg aagtcgagag gtaccccaga cgggagctcg atccgcaaag ctcgtcgatc
    14221 gaattcgacg gcaagtccct ggaattcaaa aaacaggcca cggttgtcta tttgatcaac
    14281 aaaccggccg gatatctcag cgccatgact gacgatcgcg gccggaagac gctctcggac
    14341 cttatagccg ggaagataga agagcgcgtc ttccacgtcg ggaggctcga ccagggaact
    14401 tccggactgc taattctgac caacgacgga gacctggccc atctcgtttc gcatcccgcc
    14461 tcggagatcg aaaagaccta ccttgtcaaa atacgcggaa aactcgacga cgctcaattg
    14521 cagaccgtca gagacgggat aactctggag gatggtttta ccacttcgcc cgcaagaatc
    14581 aggttagtca gaagcgacag agagaccaca actctgaggt tgatacttca cgaaggtcac
    14641 aaacgagaga taagggagat gttcaaagcc ctgggaaaga gcgttgttag cctcgtaagg
    14701 gtttcgatcg gcggtctcga catatcgctg gtgcccagac ccggagagat aaaaaagctt
    14761 ggcaaaaagg agatcgagct gatcaaaatg ggttcgaaaa gaaagagtca ggaacgatag
    14821 gagacacggc gatttgtccg tatcgctttc acaatctgcc taccattttc gggacatcca
    14881 gcacgaaagc tattcccttt ccgtcctctt ccagctttcc cgaatcgaca acggcctgga
    14941 atataccatc cagatcactc ttctttgtga gtatgagtat gatctccttc gaagactcca
    15001 cgtttatgtg caggaacttc ctgatttcgt tgtcgctcgt gccccttccg aagctcacgg
    15061 ttgcccctct ggctccggcg gcccgtgcgg cctttactat ctcctcggcc cttcctcttt
    15121 cgataatcac gattatcgcg cagacatcac tcacagtatc cctcctcaga agaacctcat
    15181 cacggtgccc agaaagagaa tcgacatggc cgcgcccatg aatgcaaacc cgaccagccc
    15241 gaatccgttg agcagagggt cgctgccctg aatgaccgaa gaaaggccta tcgagatcgc
    15301 cacggttatc ggtatgttca ccggtccggt cgtggcggcc gcgacgtcga aggctatgcc
    15361 caccatctct ttgctggaaa gtatcacaag cactgtcatc gctaccagaa aaggaaccat
    15421 tatcgtcttg atagaagtgc cgctcacgat cttgaaaacc cccagagcca tgccgagccc
    15481 gaagcccacc gccgcggagt aggtgatcga ggccgagggt atagtcccgg tagagacttc
    15541 ctcgatttcc agcgaggtcg aagtcagggc cggttcgacc agggttgcca gaaagcccag
    15601 cataaaggct atgactatta tcagaagaac gttgtccaga gccacgatgc tcctgccgaa
    15661 agagctgccc agaggtatga gggagataga ggctcctttg agaaacagaa aaagaccgaa
    15721 tacgctcagc acaataccca gaaacagact cttaaggtct ccgagtggct ttctgaagac
    15781 cacggcctgg aagattatta gaaagcccgc aagagggatg acagagcgaa cgatcatcca
    15841 caggtttttt atcagttcgg ctactttcat tggcggcaaa acctccgatc aaacgaccat
    15901 gacagatcgc ttctccgtaa tattttacca ttctttgtcg gtcttccgta aaaagaatcc
    15961 aacgacaggc attcaacgtt acaaattgaa tatttcgaat ggctctttgc cattattgcg
    16021 ggcttcgtta gcataatgta aaatcgatta aaggggtgaa aatatgatat acaggcaact
    16081 caaaaatagc ggtgtgaaaa tagccgcgat aggtctggga acatgggcga tcggaggcag
    16141 ggagagtccg gactcctcca gggacgggga gtacgtagat accatcgcac gtgcgataga
    16201 gatgggttac aatcacatag acaccgcgga atattacgcc ggaggccata ccgaggagtt
    16261 gataggggaa gccataaccc tcttcgacag gcgggagctc tttctggtct ccaaggtctg
    16321 gccgacgcac ctgagagaga aagatctgca caaagcgctg gatggtacgc tcaaaaggtt
    16381 acgcaccgac tatgtcgata tgtatctggt tcactggccc aatcccgaag tgcccctcag
    16441 ggagaccatg aaggcgatgg cagacgaggt cagggccgga agaaccaggt tcataggcgt
    16501 ttcaaacttc gatactaacc tcctcaagaa agcagtcgcc gtctcgacgg aaccggtcgt
    16561 caacaaccag gttcttttca acatcgagga tcgtcaggcg atgtatgagc tgctaccatt
    16621 ctgtcagagt gaaggcataa ccctcacagc ttattctccc ctgaagagaa atcttgtgag
    16681 cgaattcacc gggcaggcac ttgagagatt ggcggagaaa tactacgcct ctcctcagca
    16741 gataatgctg gcctggcttc taggaaagga aggagtaata gtgataccga aagcggaaaa
    16801 aaccgatcat ctgaaatcga acatcgattc gaagaacatc gttctgactc cggaggatat
    16861 gaaaatgctc gacggattag agggaggtgc gctcgtttga aagcgctcta tgaagaattc
    16921 aaaaaggctc tggacgaaca aaacaaagac agagcggtcg agatctgtat caaagccatc
    16981 gatgaagaaa aaacggacat tcccgggctt tatcttcata tattgacccc tgccctgaac
    17041 gaatgggact gtgattacga ggttgacagg ctctgcatat ggaacgaaca tgtgagaagt
    17101 tccatcgtca gaacggtaat ggagtgttgc tacccctata tcgcgcgcaa gagaagggaa
    17161 aggtccagtg atgggagaag tcctaaagtt gccgtcgtct gtccggtgga ggagtaccac
    17221 gaaattggag caagaatggt cgccgatatc ttcgcgttgg ccggttacga cgtcacgttc
    17281 gccggcgcga acacaccgca ggattccttt ctcgaggcca tgaaggtttt aaggccgctg
    17341 tacgtggccg ttagcatatc caactactac aacctgataa gcggccggtg tgtcatttcg
    17401 caaatcaagc agtggacgcc ccaatccagg ataattgtgg gggggaatgc cttcaagaac
    17461 aaccccgaat actggaaaga gatcggcgcc gacatttatc tcaccgatcc ggtcgagatt
    17521 ctgaagatgg gggagcgtga tctcaaatga aactggccct gtcgatagcc ttcaggttta
    17581 tttcctccag caaggcgcag accttgctta taatactcgg aatcgccata ggggtctcgg
    17641 tccaggtctt catagggtcg ctgatccagg gtctccagaa ggatctggta aatacaacca
    17701 taggtaactc gtcgcaggtt accataacgt cgaatacttc ggacaggaag atagataact
    17761 ggcaaaagat actggaagag gtggagtcta tacagctgcc aacacccctg acggcgctct
    17821 cagccagcgc agacgttccg gtctttataa gcagtggcga tcgcacgatt tccgtcctcc
    17881 tcagagggct tcagtttccg caggcaaatt cgatatacgg tatcgatgca aaaattatcg
    17941 aaggggagct ccctaagacg gccggcgagg ttctggtcgg taaagatctg aaagatgagc
    18001 tcggtcttca aaagggcggt cagataacca taataacccc cgacaggtcc atcgaaactc
    18061 taaggatagc gggagtcttc gatctgaaag tggcgagtct gaacaagtcg tggctgattt
    18121 cgtcaatctc gacatcccag ggtgtcggca agcttggcga ttccgtaact tcgatcgaga
    18181 tgcaggttga ggaagttttc aaggccgacg aagacgccac tgctatagca tcggcactgg
    18241 ctgacgcgaa actggacgtc accaactgga aggcgcagaa cgcggatcta ctgaccggcc
    18301 tcaacgggca gagcatatcg agcataatga tacaggtctt tgtgataatc gccgtttcgc
    18361 ttggaatagc gagcgttctc gcgataaccg ttgtccagaa atccagacag ataggtattc
    18421 tgaaggctat gggtttgaag gattcagttt ccagccaggt cttcctcttc cagggattgt
    18481 ctctcgggat aatcggcgcg gtcgtcggta tagcctttgg cttcctgctt atactgatgt
    18541 tcagcacctt cgcggtcggt cccgacggcg agccggtcgt cggtatctac gtgaactacg
    18601 gatttatcat gttatcggcc ttcatagcca tcggcgcctc aaccatagcc gcgctcatcc
    18661 cggccagaag atcgtcgaaa ctcagccccg tcgaggtgat aaggaatggc taatataatc
    18721 gaattgaaag aagttagaaa ggtgtacgga actgtaatca agaccgaagt tcttcacggg
    18781 atcaaccaga gtttcgaagg aggcgccttc aattctat
//
LOCUS       DAOOLY010000011        18531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3074_length_18531_cov_5.546060, whole genome shotgun sequence.
ACCESSION   DAOOLY010000011 DAOOLY010000000
VERSION     DAOOLY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 18531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18531
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_18531_cov_5.546060"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLD15_01190"
     CDS             complement(<1..446)
                     /locus_tag="PLD15_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPB62540.1"
                     /translation="MDRVIVVGNVNVDIIAGSIDRWPAWGTEIGVDSIEVRIGGQAAN
                     SAVVLSRLGFPVEVVTVAGDDFIGKGFRARFSELGIDVSNFELLKGRTPFSIAITHSS
                     NERSFFSNESVMRSLDVDFVADRLRGTKGAHILFCGINVLRGLHED"
     gene            792..1604
                     /locus_tag="PLD15_01195"
     CDS             792..1604
                     /locus_tag="PLD15_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62541.1"
                     /translation="MKRLLVLVVCTVMLLTISGCFPRVKPLPKAVLIDDTHNNIFASS
                     IDEYSTSASSDYFEGIITILYSRGYEVAYSSEAGFNPQNYAIVILSMPLQAYTPTEIA
                     ALNAQLGKKGKIIMMDLLVEVPPSSTFFLDALSDGLNTGIGFVWGLVTDTVNNIGGNK
                     LVITSDNFGAHPIASTLSNKIAMMFTTKLTVEGNASAVVYAENSAVFLTYVSSLEEQA
                     SIPQASDPIPIVAASSVNGGKVVAISSAYAFHGTLMGIVPGNTELFEAIVDW"
     gene            1832..3091
                     /locus_tag="PLD15_01200"
     CDS             1832..3091
                     /locus_tag="PLD15_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62542.1"
                     /translation="MKRRYLALLVIPLLIMLTGCPFLNKLPLWTLIPNIIRNIGQVVN
                     INLSTYCSDPDGDPLVFTVVSGPGTISGNTYSWTVSGSIGVVTITISASDGKGQSSTS
                     FTITVKGAPNTPSNPSPANNATNQNYPNLTLSWTGGDPDGDSVTYDLYFGTASNPPLY
                     TSNLTSTTYNKAGLLSNTTYYWKIVAKDGVNTKTGPIWAFSTKPYTIVNDSFESRPLG
                     NLSAATLPWATYTKIDPGYSYISVNLGYGGSKGLTFVDSNEAGNSRISRNISALKKGT
                     VQFYLRAAANGSIGFRDNTGWVPYVCLGDMGSGYGMYSWNESTGVFTKIMNVTSNTWY
                     DIYIYFDLNAGQNYYRVYVNDVLRRTEYLTGTFSMTSFSFLTFTNEVCSWADIDNVVI
                     TALQPGYSTAGEAGFEEFSGSTDLSTR"
     gene            complement(3176..4378)
                     /locus_tag="PLD15_01205"
     CDS             complement(3176..4378)
                     /locus_tag="PLD15_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62543.1"
                     /translation="MSKFRLFSRADLQLEVVILIIAGLIVSITGILLFPVATGSLPYY
                     ENGLYGLLLVMFALQIVSLGKTPFGDMNRSWLLVTAGIAVAAVGIVTCFVPDIFSEIP
                     RLLLMLFFGPGGFLLFMQMVLSRDRLTNWLKYGGIFRHLIAGCSLVYLLSMVIALLLL
                     NRNLLTTQATAAVVLAYGVSIIYLALVLRKIYLAYPQAEKKGGERVDLPMDRAMILLT
                     GVFMVLLGLLLIPVNLGILPFSGSAQLGLLMVFFSVQMLASGSTPIGPFPRTWLMILL
                     GLLFAALGTISCVIPEILVSPLTVLIGILNILGGLINLGKFAFQSLKGRGKPQASLPP
                     IVHRLVLTQLVLSLLSIVFGTSMLISSLLPGLVIGIVLTANGVVLLYLLYTLSVIDRM
                     SKAVENTK"
     gene            complement(4380..5186)
                     /locus_tag="PLD15_01210"
     CDS             complement(4380..5186)
                     /locus_tag="PLD15_01210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB62544.1"
                     /translation="MSEYFANKVAVVTGAASGIGLGLTEHLLSRGAMAVFMSDVKLEN
                     LDKEAKRLESNFPGKVFPVLSDVTKLEQIEACINKARDFDGHLDFLFNNAGIGMTLPT
                     EQVTFDLWKFVIDINLMGVIYGTYTAIPIMRKQGSGHIVNTASIAGLVPVPYQALYAA
                     TKSAVKTMSESLQYELETDGLQFSVICPGNVRTAIFGNLTPPPDSISVDEAVEYIFDE
                     MEKKALLIVLPQAYRDFDIAYRTNREEFDKFIRQLGAERRENYRTKGTYY"
     gene            complement(5201..6007)
                     /locus_tag="PLD15_01215"
     CDS             complement(5201..6007)
                     /locus_tag="PLD15_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HPB62545.1"
                     /translation="MSRRGKFKLKDDHIYKMPVHFGGDPFYPVRTVYRDMTGISIQFE
                     TDQEALLEIIPHDFELLEPVVNVQFSNCRDVDWMAGGEYRLIQVASPVRYVGNSEGLE
                     GQYVFVIWENKTCPIIGGREEDGMPKVFADISCERHLDDYWFTAASYESYTFLKLDFM
                     KTGEFDDETVEEVNKNPNINFFAWRYIPNLGKAGASLSHATLYPQEAYFSKIWTGNGN
                     LEWTKLNYEKHPLQCRIISTLAELPVVRYTDAKMTKNSARLNVGDSRILP"
     gene            6396..7082
                     /gene="lipB"
                     /locus_tag="PLD15_01220"
     CDS             6396..7082
                     /gene="lipB"
                     /locus_tag="PLD15_01220"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPB62546.1"
                     /translation="MRRKCMEVRIEGLTGYSEGLSLQKRALERVESSEVDGVLIVLEH
                     RPVFTTGRSGGMENLLVDGDELTRLGIELHRADRGGNITYHGPGQLVAYPVFNLEKWR
                     KDLPWYVSSLEEVVIQVLSDYGIEAVRKPKYRGVWVGERKIAAVGIAVKRWITTHGLS
                     FNIEVDKSHFELINPCGLKGLAISSLNNFVSPVNFSEVVDKMEEKFSLVFDTKFIKIP
                     RGWLDGDIYA"
     gene            7075..7944
                     /gene="lipA"
                     /locus_tag="PLD15_01225"
     CDS             7075..7944
                     /gene="lipA"
                     /locus_tag="PLD15_01225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPB62547.1"
                     /translation="MRERKPDWLKDMIALDRNDLREVKLMLRGLNLHSVCEGARCPNI
                     GECFASKTATFMILGDTCTRNCRFCAVRKGIPSSPDRFEPENIAKASNLMGLKHIVVT
                     SVTRDDLPDGGANQFKEVIRTLRRECPGTTVEVLIPDLRGDWKALAEIVGEHPDLLNH
                     NLETVPSLYDSVRPQADYGRSIKLLKRAKEMDSALITKSGIMVGLGEKDEEVLGVMDD
                     LLEAGCSVLTIGQYLRPSLEHLPVVEYVRPEKFEFYRKRALEKGFSFVSSGPLVRSSY
                     HAAELFTESKTRK"
     gene            7988..8410
                     /locus_tag="PLD15_01230"
     CDS             7988..8410
                     /locus_tag="PLD15_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB62548.1"
                     /translation="MKIGIVVYSQTGHTLSVASKLAEKLASLGHETSLKRLESEDFKI
                     DEFETFIFCSPVHGGEPAQQMKDYINRIPSLEGKKAACLVTGVFPANMGRNKTLESMK
                     RLCESKGATVCGVASVGWWSLKRSVQIREAVESIASCF"
     gene            complement(8578..10035)
                     /locus_tag="PLD15_01235"
     CDS             complement(8578..10035)
                     /locus_tag="PLD15_01235"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HPB62549.1"
                     /translation="MEEKMKLTNEVGAPVVDNQNSMTAGPRGPMLLQDVWFLEKLAHF
                     DREVIPERRMHAKGSGAYGVFTVTHDITMYTKAKIFSEIGKQTEMFVRFSTVAGERGA
                     ADAERDIRGFAMKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHVVKRDPRTNLRSAK
                     NNWDFWTLLPEALHQVTITMSDRGIPYSYRHMHGFGSHTFSMINANNERVWVKFHLVT
                     EQGIKNLTDAEAEAIIGKDRDSHQRDLYESIERGEFPQWTMFIQVMTMEEAEKMSYNP
                     FDLTKVWYKKDFPLIEVGYFKLNRNPENYFAEVEQAAFNPAHIVPGIGFSPDKMLHGR
                     LFSYGDAQRYRLGINHHLIPVNAPKFPANSYHRDGQMRVDGNAGSKLSYEPNGYGEWQ
                     DQPQLKEPPLPLYGPADNWNFREDDDDYYTQPGKLFRLMNPEQKEALFCNTARAMGDC
                     PREIKIRHIGNCLKADPDYGKGVAAAMNIPLDEVK"
     gene            complement(10042..10476)
                     /locus_tag="PLD15_01240"
     CDS             complement(10042..10476)
                     /locus_tag="PLD15_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPB62550.1"
                     /translation="MKRSIEELISYLKERELRPSYQRLKILGYLLENRNHPTADEIYE
                     SLHSEIPTLSKSTVYNSLNVFVQARLVKQLTIEDSDTRYDIVTQDHGHFKCELCGRIY
                     NFKINIDGSDTPDLKGFNIKDRNVNFTGTCPSCLKGKEIRGR"
     gene            10773..11312
                     /locus_tag="PLD15_01245"
     CDS             10773..11312
                     /locus_tag="PLD15_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPB62551.1"
                     /translation="MAEKKSFNWISLLVGIVLIVGSIFAFMNPVATFLTLAIMLGIVA
                     VVRGLMLIIAFFRVNDRTTVKVWFFLIVGILLTILGVIFLFRPAFAAVVFAIMVAVWF
                     IVDAINNLINIDRIKPAGKGIYFLSIVLNLLLLVGGIIIALHPWIVGISIPIIIGMAL
                     MASGIQHLVMAFFGPRDLV"
     gene            complement(11406..11999)
                     /locus_tag="PLD15_01250"
     CDS             complement(11406..11999)
                     /locus_tag="PLD15_01250"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPB62552.1"
                     /translation="MKRKISGFLLAVLMISGVVLAGVADSTTIVKTPMVATVMGDGSG
                     SIVLELVCMRYGIECVNVLDMTAETLGERLEQSNGPKTLVIATGAMTEGDLCTVCGAI
                     EIDIERELERIEKLIEKARASGLSIVGLHIEGGIDIAERNFNRSIDAIMPYVDLVLLV
                     TPDEVSSYFKKLSEEKGTPMIKVDEALKIGDVLKELL"
     gene            complement(12186..13586)
                     /locus_tag="PLD15_01255"
     CDS             complement(12186..13586)
                     /locus_tag="PLD15_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB62553.1"
                     /translation="MSKKGKIKILFAEDLPTDVDLARWEISRVFPEIEAAVVDTEHDF
                     MEKLKEFSPDLVISDYLMPGFDGMRVIELVLKNAPELPVIILTGSMNEDTAAECMKAG
                     AIDYVIKEHMARLPYAVVEALEKKEIKIAKEKAEKALIESEKKFRLLAEKANDLIYRY
                     ELYPEEGFSYVSPASVRITGYTPDNFYKDPDLITRIVHSEDRDRLLNITNYYITHSEQ
                     RETLTMRWVRKDGKVVWIEQQNVRIFDDNEKLIALEGIARDVTERHEAAERIREAFYS
                     IITVVSDILTARDPYIEYHHRNVSRLSSKIAEKIGLDDTRIEAVKVAGLLHDIGKIAI
                     PAEILSKPGKLNDKEFSLIQQHSIVGYNILRNTRLPWPVAEIIHQHHERLDGSGYPGK
                     LKGNEICVEARIIAVADVVEAMTSHRPYRPAHRMETALEEIRGKSGTAFDSRIVEVCI
                     GLIENGFDFKQDASKN"
     gene            complement(13595..14038)
                     /locus_tag="PLD15_01260"
     CDS             complement(13595..14038)
                     /locus_tag="PLD15_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB62554.1"
                     /translation="MIDSVVEILLVEDNPEDIELTLRALKKSKLANNIVVAEDGEEAL
                     DFIFARGKYSDRSVICSPKVILLDLKLPKVSGIEVLREIKKDPRTSIIPVVILTSSTQ
                     EVDIEESYKLGANSYIVKPVNFENFIQSLRQMNDYWLVLNRLPSR"
     gene            complement(14050..15843)
                     /locus_tag="PLD15_01265"
     CDS             complement(14050..15843)
                     /locus_tag="PLD15_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB62555.1"
                     /translation="MSHERSSLDPMERDFRPSTEVERNLNPILDIDIDGKTTYQNAAA
                     TSFLNSSGLKDPVAFLPEELPQEIWKSKEPSPFNYEKTIGEKIFFVSLNPLPGGDIAR
                     IYAFDITQIRTLERQQLETKELLAKVTGSVTDMLYALDKELRYIYWNSASEVMTGLKS
                     IDVIGKHVTDVFPEDATTAKAMASYRRVLQSGKPESSVFEYKSMRKTSILEINIYPLG
                     EGISVFARDITEQKNAERKLEIERKKLRQILDYIPEGIYMVNESYDIEYVNPVLIEKY
                     GPIEGKKCYEFFLKFDAPCPWCKNDQIHSGQPVRWERRSERSGRIYDLIDIPVFNDDG
                     SISKLQVVHDITDMRTYQEKVERLNVELEARVAERTTQLEAVNKELEAFAYSVSHDLR
                     APLRAVDGYSRIIEEDYVNVLDSEGLRLFGVIKRNIKKMDQLILDLLALSRVNRTELV
                     KSKIDMIAMVNSLFNEVVTVELREKVKFTVQNLPEVYGDSVMMKQVWINLLSNALKFS
                     ATREVITIEVGGYSENGTNTYYVKDNGVGFNQKYANKVFEIFQRLHSSKEFEGTGVGL
                     SIVQRIIHRHGGSVRAEGEENQGATFYFSIP"
     gene            complement(15974..17143)
                     /locus_tag="PLD15_01270"
     CDS             complement(15974..17143)
                     /locus_tag="PLD15_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB62556.1"
                     /translation="MSIETFDYFLPTRVIFGCGTIGKVGQHSRRLGKKALIVTGRHST
                     KKTGLLDKVIGLLKEEGISSVVFDEIVPNPLSTSIDRGASIAVKERCDFIIGLGGGSP
                     IDSSKLIALVAKDGGKCWDYTGAGGGRKPKAALPVIAIPTTHGTGTEADPFAVVTNPE
                     TNEKIGVGFDEIFPAVSIVDPELMLSLPPEQTAATGMDAFYHSIESYINLNHQPASDL
                     LALESMSLINHYLPIAYENPNDLDARVALAWASTAAGICETLSGCVANHSLEHPVSGH
                     YNATHGAGLCATGPSLLEYIRPHIAERLAEVARVMGAPESVIGVEELSKMAIVLLRRL
                     QKNVGLDISLGELGVESSKLSILAEDAMRTMGGLVSVTPGNLKTEDLRNIYEMSM"
     gene            complement(17164..18318)
                     /locus_tag="PLD15_01275"
     CDS             complement(17164..18318)
                     /locus_tag="PLD15_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB62557.1"
                     /translation="MNILASIEHFVQLRFGDFFSSIEAFYLKDILILIQGILELGHHS
                     ISSIARDPLNKVAHTTLTRFVNGHPNFWKNFEKLIQKVILSTSSEKMILVVDDTQLAR
                     RSKKIPFTTPTYDHNQKRYCQAQVLLTVGRVSDGFFPLEMMFSNSNGGPTKIERLIDW
                     LKENEIRDAVLLGDSWYTNSHVIESCKLWFGITFIGKVRGNLLFKTEDSIIRVSHYQN
                     SVYDFSEEVVIRDRTVRFHQRVVRFKSVRVPLKIVVAELDDGRRTTLISSDTELSSED
                     IFLYYLHRWSIESYFKTAKQHFSLGKAGLSTEDGHKHWIILVILAYLIFNDLQRFILE
                     KTKSETKRYKVFETIQRAISMLRSLLLEQVKIDFHLLDSCFRSPSLAPFY"
BASE COUNT         4420 a         4675 c         4488 g         4948 t
ORIGIN      
        1 ttatcttcat gcaagcctct gagtacattg atcccgcaaa aaagaatgtg ggcgcccttt
       61 gttcctcgca gcctgtcggc cacaaaatcg acatcgagag atctcatgac gctctcgttg
      121 ctgaagaagc ttctctcgtt actgctgtga gtgatggcta tggaaaaggg tgtcctgccc
      181 ttcagcaact cgaaattaga tacatctatg cccagctcgg agaaccttgc cctaaaacct
      241 ttaccgatga aatcatcccc tgcgaccgtg acaacttcca ccggaaaacc gagtcgagaa
      301 agcaccacgg ctgaatttgc cgcctggccg cctatcctga cctctatgga atccactcca
      361 atttccgtac cccaggcggg ccacctgtcg atcgatccgg ctatgatatc gacattcacg
      421 tttcccacaa cgattactct atccaattcg atcacactcc ctcagggttt ataaagtgta
      481 cgatgattat accatccacg aaaggcgatt tatggactgg tgataggccg cactaaccta
      541 gtgatcgccc gatatttccg tatcgctttt tctagttcgg cactgtgtct gttgagtatt
      601 gatattgagg aataaatcga atcgtactca taaacaaaca ttattcataa ccatcgactg
      661 tttttttaac tgctttcgca atcttttcgt ccacaaatga accttcgttt gaccggttta
      721 ataacccata cttcgatcct tattgatcat ctctttcttt cgcttgtatg atctcgatgg
      781 gaggtgagaa aatgaaacga ttactggtac ttgtagtctg cacagtaatg ctgttaacga
      841 tatcaggctg ctttcccaga gtcaaaccgc ttccgaaggc tgttttgatc gacgataccc
      901 acaacaatat tttcgccagt tcgatcgatg agtattccac aagcgcttca agcgattatt
      961 tcgagggcat aattaccatc ctttattcga gaggctatga agtggcttac tcgtcggagg
     1021 cgggattcaa tccacagaac tacgcaattg tgattctatc gatgcccctc caggcctaca
     1081 caccgaccga gatagccgct ctcaacgccc agctcggcaa gaaaggcaaa ataataatga
     1141 tggatttgct cgtcgaagta ccgccgtcaa gcaccttctt cctcgatgcc ctttcagacg
     1201 gactgaacac cggtataggc ttcgtgtggg ggctggtaac ggatactgta aacaacatcg
     1261 gcggcaacaa gctcgtgata acatccgata atttcggcgc tcatcctata gcttcaactt
     1321 tatcgaacaa aatcgcgatg atgttcacga caaagctcac agtagaggga aacgcctccg
     1381 ctgtcgtcta tgctgagaac tctgccgttt tcctgacgta tgtttcatcg cttgaagaac
     1441 aggcttcgat tccacaggcc tccgatccga taccaatagt cgcggcatcc agtgtcaacg
     1501 ggggcaaggt cgtcgccatt tcgagcgctt acgctttcca cgggactttg atgggaatcg
     1561 tacctggcaa caccgaactc tttgaggcca ttgtcgactg gtagctcttt aagaaaatcg
     1621 actctgcgag tcatcatctt tgcatgagta gagcttctca ggcttcataa acgccgggtc
     1681 ggaatatttt tctcgaccgg gtggagcgag cactctataa aagagcatca gtattgatac
     1741 tttgggttta gaatcggtta gatcaaatca gaaggcgcag gttgcgaata tcggtattgc
     1801 tcgcttcaca cattcgcgag gaggtgttgg tatgaagaga agatatctgg ctctactggt
     1861 aattccgctt ttgataatgc tcacaggttg tccctttctc aacaagctgc cgttgtggac
     1921 cttgattccc aacataatca gaaatatcgg acaggtggtc aacatcaatc tttcgactta
     1981 ctgttccgat cccgacggcg atccacttgt cttcactgta gtgagcggac cgggaacgat
     2041 atcgggaaac acttacagct ggacggtcag cgggtcgata ggcgtggtca caattaccat
     2101 aagcgcctct gacggaaaag ggcagtcaag cacgagtttc actatcaccg tcaaaggagc
     2161 gccaaacact ccatccaatc catcgcctgc gaacaacgcc accaaccaga attatcccaa
     2221 tctcacactt tcgtggaccg gcggagatcc cgacggcgat agcgtaacgt acgatcttta
     2281 ttttggaacg gcttcaaatc cgccactcta tacctcgaac ctgacctcga ccacttacaa
     2341 caaggccggg ttgctgagca acacaactta ctactggaaa atagtggcca aggacggcgt
     2401 caacaccaag accggcccca tatgggcttt ttcgacaaag ccctacacga tagtcaacga
     2461 cagcttcgaa tcgcgtccgt tgggaaattt gagcgccgca actctcccct gggcgacata
     2521 cacaaaaatt gatccgggat actcgtatat ttctgtcaat ctcggctacg gtggctcgaa
     2581 agggctcacg ttcgtcgact ctaatgaagc aggcaattcg aggatatctc gaaatatttc
     2641 ggctctgaag aagggaactg tccagttcta tctaagggcc gccgcgaatg gtagtatcgg
     2701 gttcagagac aataccggct gggttccata cgtatgtcta ggagacatgg gaagcggtta
     2761 cggtatgtat tcctggaacg aaagtacggg agttttcacc aagatcatga atgtgacctc
     2821 gaacacctgg tacgatatct atatctattt cgatctcaac gccggtcaga actactaccg
     2881 ggtctatgtt aacgatgttt tgagaaggac ggagtatctc accggcacgt tcagtatgac
     2941 cagtttctcg ttcctgacgt ttacaaatga ggtttgcagc tgggccgaca tagataacgt
     3001 ggttatcacc gccttgcagc ctggctactc aacggccggg gaagcggggt ttgaagagtt
     3061 ctccggctcg accgatttat ccacaagata aatggagcat aaaaagcata ccgcctctgc
     3121 ggtatgcttt ttttcatgtc aaatatacaa aacagtggtt cattctagac cgagactatt
     3181 tggtgttctc cacagctttg ctcatcctgt cgatgaccga gagagtgtac agcagataca
     3241 gcagtaccac tccgttggcc gtcaatacta tgcctataac gagacctgga aggagagatg
     3301 aaatcagcat cgaagtcccg aacactatcg aaagaaggct cagaacaagt tgagtcaaca
     3361 cgagtctgtg tacgatcgga ggtagcgaag cctgcggttt tcctctgcct ttcaggctct
     3421 gaaaagcaaa ctttcccaga ttgatcagac ctccaaggat gtttagaata cctataagaa
     3481 cggtcagcgg ggaaacgagg atttcgggta tcacgcacga gatggttccc agcgccgcga
     3541 acagaagtcc gaggagaatc atgagccagg tgcgggggaa gggacctatc ggtgtgctgc
     3601 cggaggccag catctgaacg gagaagaaga ccataagcaa tcccagctgt gcgctcccgg
     3661 agaagggcag tattccgagg ttgacaggga tgagcaacag acccagcagt accatgaaaa
     3721 cgccggtaag caaaatcatc gccctgtcca tgggaagatc gaccctttcc ccgcctttct
     3781 tttcggcctg ggggtaggcc agatagattt tacgcagaac gagagccaga tagattatgg
     3841 acaccccgta ggccagcacg acagccgcgg ttgcctgtgt tgtgagcaag ttacggttca
     3901 acagaagcaa agctatcacc atcgagagaa gatagaccag cgagcaaccg gcgatcagat
     3961 ggcgaaagat cccgccgtat ttcaaccagt tcgtcagcct gtccctggaa agaaccattt
     4021 gcatgaacag caaaaaacct cccgggccga aaaaaagcat caggagcagt cgcggtatct
     4081 cgctgaaaat atccggaacg aaacaggtca ctattcccac ggctgcgacg gcgataccgg
     4141 cggtgacaag aagccaggag cggttcatat ctccgaaggg agtctttcca agcgatacga
     4201 tctggagcgc gaacatcacc agaagcagac cgtacaggcc gttctcgtaa taaggaagcg
     4261 atcctgtcgc gacgggaaaa agtaaaatgc ccgttatcga cacgatcaag cccgcgatta
     4321 tcagtatcac tacctccagc tggagatcgg ccctgctgaa caaccggaat ttactcatat
     4381 caataatagg ttcccttcgt gcgatagttt tcccgtcttt cggcacccag ctggcggata
     4441 aacttgtcga attcttccct gttggttctg taggcaatgt cgaaatctcg atacgcctgg
     4501 ggcagtacta tcaggagagc ctttttctcc atctcgtcga agatgtattc gaccgcctca
     4561 tcgacgctta tcgaatcggg tggaggcgtc aggttgccga atatagccgt acggacattg
     4621 ccgggacaga ttacgctgaa ctgaaggcca tcggtttcga gttcgtactg cagcgattcg
     4681 ctcatggttt tcaccgcgct cttggtggcg gcgtacaatg cctggtatgg cacagggacc
     4741 aggcccgcga tggaggccgt gtttactatg tggcccgaac cctgtttcct cattatcgga
     4801 atggccgtgt aagtgccgta gataacaccc atcagattga tatcgatgac gaatttccag
     4861 agatcgaagg tgacctgctc ggtgggcaga gtcataccga tacctgcgtt gttgaagagg
     4921 aagtcgagat ggccgtcgaa atccctggcc ttgtttatgc acgcttcgat ctgttccagc
     4981 ttcgtcacat cggaaagcac cggaaagacc ttcccgggga aattcgactc cagtcttttg
     5041 gcttccttat cgaggttctc cagtttaacg tcgctcatga atacagccat ggctcctctg
     5101 gagagaaggt gttccgtcag gcccaggccg atacccgaag ccgcgccggt tacaaccgca
     5161 actttgtttg cgaagtattc gctcatctat ctccctcctt tcagggtaga attcttgaat
     5221 cgccgacgtt cagtctggca gagtttttgg tcattttcgc atcggtgtat ctgacaacgg
     5281 gcaattcggc gagagtggag attattctgc attgaagggg atgtttttcg taattcagct
     5341 tcgtccactc caaattgcca ttgccagtcc agattttact gaaataggcc tcttgcgggt
     5401 atagtgtggc atggctcaga ctcgccccgg ctttgccgag attgggtatg taacgccagg
     5461 cgaagaaatt gatattcggg ttcttgttta cctcttccac ggtttcatca tcgaactcgc
     5521 cggtcttcat gaaatcgagc ttcaaaaagg tgtagctttc gtaactggcg gccgtgaacc
     5581 agtaatcatc gaggtgtcgc tcgcaggaga tatctgcgaa gactttgggc attccgtcct
     5641 cttcccgtcc accgatgatc ggacaggtct tgttctccca gattacaaag acatattgac
     5701 cttccaatcc ttcggaattt cccacatatc tcacaggcga agcgacctgt atcaatctgt
     5761 actcgccacc cgccatccag tctacatcgc ggcaattcga aaactgaacg ttgaccaccg
     5821 gttccagaag ctcgaaatca tgcgggatga tttccagcaa ggcttcttga tccgtttcaa
     5881 attgaatact gatacccgtc atgtctctat acacggttct caccgggtag aagggatctc
     5941 ccccgaagtg aaccggcatt ttgtagatgt gatcatcctt cagtttgaac tttcctctcc
     6001 tggacatcgg tacctccttt tagactgcaa gcaaaaccga gaacctgcac cgaaatcgca
     6061 aggcctgtcg attgcaggtc gcgacgggtt ggcgtttcct tctattttta tgtaaagagt
     6121 gtacagaata gcctcaagtt gcgagcgttt gtttgtctta cacattacac gtgaatatga
     6181 aagcatattc gtccgggaaa acaatctcct ctcgagagtg tttagagtcg actatatttg
     6241 ctttgagacg atagaggtcg atagttttac tatctcatat tacaatattt ctcgtagttt
     6301 caagcaatcg aagaactctc tggaattcac gatatccgaa acgccttgaa ataatagtcc
     6361 cgaacggtgc tacaatttca aaaggtggag gtcttatgcg tcgaaaatgt atggaagtta
     6421 gaatcgaggg cttgaccggt tatagcgagg ggctctctct acaaaaaaga gccctggaaa
     6481 gagttgaatc atctgaagtc gatggtgtgc tcatcgtgct ggaacacaga ccggttttta
     6541 cgaccggcag gtccggaggc atggaaaacc ttctcgtcga tggcgatgaa ctcacgcgac
     6601 tgggcataga attacaccgt gccgacaggg gagggaacat cacgtatcac gggcccgggc
     6661 agcttgtggc ttatccggta ttcaacctgg aaaagtggag aaaggatctt ccctggtacg
     6721 tttcctctct cgaagaggtc gtgatccagg ttctgtcaga ttacggaatc gaggcggtga
     6781 ggaaaccaaa atacagggga gtatgggtgg gcgagagaaa gatcgcggcc gtcgggatcg
     6841 ccgtaaaaag gtggataacc acgcatgggc tgtccttcaa catagaagtg gacaaaagtc
     6901 acttcgagct catcaatccc tgcggtttga aagggttggc catctcttcg ctaaacaatt
     6961 ttgtatctcc tgtgaatttc tccgaagtgg tggataaaat ggaggagaag ttctccctgg
     7021 tcttcgatac gaagttcata aagatcccca gaggatggct ggatggtgat atatatgcgt
     7081 gagagaaaac ccgattggct taaggacatg atcgcccttg acagaaacga cttgagagaa
     7141 gtgaagctta tgcttcgtgg gttgaatctg cattcggtat gcgaaggggc cagatgtccg
     7201 aacatcgggg agtgcttcgc ctcgaaaact gccactttca tgatactggg cgatacgtgt
     7261 accagaaact gccgtttctg cgcggtgagg aaggggattc cctcttctcc ggacagattc
     7321 gagccggaaa atatagcgaa agcctctaat ttgatgggtt tgaagcacat cgtcgtgacg
     7381 tcggtcacca gggacgatct gccggacggc ggtgcgaacc agttcaaaga agtgattcga
     7441 actctcagaa gggaatgtcc gggtaccacg gtggaggttc tcatacccga tctacgcgga
     7501 gactggaaag cgcttgcaga aatagtggga gaacacccgg acttgttgaa ccacaacctc
     7561 gaaaccgttc cgtccctcta cgattccgtc cgccctcagg ccgattatgg gcgttcgata
     7621 aagcttttga agagggcgaa agagatggac tccgcgctta tcaccaagtc cggaattatg
     7681 gtcggtctcg gagagaagga tgaggaggtg ctcggggtaa tggacgatct tctggaggcc
     7741 ggctgcagcg tgcttacgat tggacagtat ttacgtccat ctctcgaaca tctgccggtt
     7801 gtggagtacg tgaggcctga aaagttcgaa ttttacagga aaagagccct cgaaaagggc
     7861 ttctcctttg tgtcgtccgg accgcttgtg aggagttcgt atcacgcggc cgaactattc
     7921 acggaatcaa aaacgcgtaa gtaacggaat atagcgtatc agtgtgtgaa acgggaggtg
     7981 gtgggtcatg aagataggca tcgtcgttta ctcgcagacg gggcatacgc tatcggtggc
     8041 gtcaaaatta gccgagaagc tcgcatctct gggacatgaa acttctttga agaggctgga
     8101 gagtgaagat ttcaaaatcg atgagttcga gacctttatc ttctgctcgc ccgtacacgg
     8161 tggcgagcct gcccaacaga tgaaggacta tatcaatcgg attccctctc tggaaggtaa
     8221 aaaagcggcc tgtctggtaa ccggcgtctt tccggcgaac atgggcagaa acaagaccct
     8281 ggaaagcatg aagaggcttt gcgaatcgaa aggcgccaca gtttgcggtg tcgcgagtgt
     8341 tggttggtgg agcctcaagc gaagcgttca gataagagag gccgtagaaa gcatcgcctc
     8401 ctgcttctaa agcgcagcat cccgcaaagc cggttgtctc tacccgatct tgattgatat
     8461 agcgggcaaa agtcgctcgc ccctacacga gtttattatg gttttacctc gatatacgat
     8521 tcatagtgtc ttcgtagggg cgcgcgacgt acacccgttc atattcaaat agagccttta
     8581 tttcacctcg tccagaggta tattcatggc cgccgctaca ccctttccgt agtccggatc
     8641 ggccttcaga caatttccta tatgcctgat ctttatctct ctgggacagt cgcccatggc
     8701 gcgggccgtg ttgcagaaga gcgcctcttt ctgctcgggg ttcataagcc tgaagagttt
     8761 ccctggctgt gtgtagtaat cgtcatcgtc ttctctgaag ttccagttgt cggccgggcc
     8821 gtagagaggc agcggcggtt ccttcagctg gggctgatcc tgccattctc cgtagccgtt
     8881 gggttcgtag gagagtttgc tgccggcgtt accgtcgacc ctcatctgac cgtcgcggtg
     8941 gtagctgttg gccgggaatt tcggcgcgtt gacgggtatc aggtgatggt tgatgccgag
     9001 tctgtaacgt tgcgcatcac cgtaggagaa gagccttccg tgtaacatct tgtcgggcga
     9061 aaagcctatg cccggtacta tgtgcgccgg gttgaaggcc gcctgttcga cttcggcgaa
     9121 gtaattctcc ggattacggt tgagtttgaa ataaccgacc tctatcagcg ggaaatcctt
     9181 cttgtaccag accttggtca gatcgaaggg gttataggac atcttttcgg cctcttccat
     9241 cgtcatgacc tgtatgaaca tcgtccactg cggaaactct cccctttcga tactctcgta
     9301 aaggtctctc tgatgactat cgcgatcttt gccgatgatc gcttcggctt cggcgtcggt
     9361 caggttcttt atcccctgct ccgtcacaag gtgaaacttg acccatacgc gttcgttatt
     9421 ggcgttgatc atgctgaaag tgtggcttcc gaaaccgtgc atatggcggt atgaataggg
     9481 gattcccctg tcgctcatag ttatcgtgac ctggtggagc gcctcgggca acaacgtcca
     9541 gaagtcccag ttgttcttgg cactcctcag gttggttctg gggtcgcgtt taacgacatg
     9601 gttcagatcc gggaacttca aaggatcgcg caagaagaag acaggcgtgt tgtttcccac
     9661 cagatcccag ttgccctcct ctgtgtagaa cttcatcgcg aatcccctta tatcgcgctc
     9721 ggcatcggcc gctccgcgtt ctccggccac cgtcgagaag cggacgaaca tctcggtctg
     9781 ctttccgatc tccgaaaaga tcttcgcctt ggtgtacata gtgatgtcat gagttaccgt
     9841 gaagactccg taggctcccg atcccttggc gtgcatcctt ctctcgggga tcacttccct
     9901 gtcgaaatgg gccagcttct ccaggaacca gacatcctgc aacagcatgg gacccctcgg
     9961 cccggccgtc atcgagtttt gattgtcgac caccggcgcc ccgacttcgt ttgtcagttt
    10021 catcttttct tccataaacc actacctccc tctgatctct ttgcctttaa ggcatgatgg
    10081 acaggtgccc gtgaagttta cgttcctgtc tttgatgttg aaacctttca gatccggtgt
    10141 atcagaaccg tctatgttta ttttaaagtt gtatatcctt ccacagagtt cgcacttgaa
    10201 gtgaccgtgg tcttgtgtga cgatatcgta cctcgtatcg gagtcttcta ttgtcaattg
    10261 cttcaccagg cgggcctgga cgaaaacgtt caggctgttg taaactgtgg atttagagag
    10321 ggtcggaatc tcgctgtgca ggctctcata gatctcatcg gccgtcgggt ggtttcgatt
    10381 ctccagaaga tatcctagta tcttcaacct ctgataggat ggcctcagct ctctctcttt
    10441 aaggtaactt atcaactcct ctatggatcg cttcatgtcg cctcccttca gggactggac
    10501 aaaactcgaa ttgtgtttaa aataagaacc attcttattt tataattatt ataacatatc
    10561 taatagagtt ttcacatcct ccatataaag agcggcttaa aaaactacac cgtcaagact
    10621 gtgcgcataa aatgaatcgt ccttcaaaaa cttgacttgc aacgtcccaa atatatataa
    10681 tcatcctatt tgagagtaat gctgatttac tgaaccagat gaactaaaag attttccggt
    10741 caaaaaacgt tgcaccaaaa ggggtgggtt gtatggcaga aaaaaagagt ttcaactgga
    10801 tctcgctact ggtagggata gttttgatcg tgggtagtat ttttgcgttt atgaacccgg
    10861 tcgccacttt cctgactctg gctatcatgc tcggcatcgt cgccgttgtt cgtggtctca
    10921 tgctgataat agcctttttc agggtgaacg acagaacgac cgtcaaagtg tggttttttc
    10981 tcatagtcgg catcttgctc acgatcctcg gcgtaatctt tcttttcaga ccggccttcg
    11041 ccgcggttgt tttcgcaata atggtcgccg tctggttcat agttgatgcg atcaacaacc
    11101 tgattaacat cgatcgcata aaacctgccg gaaagggcat ctattttctc agtatcgtcc
    11161 tcaacctctt actgctggtc ggtggtatca taatagccct gcacccctgg atagtgggaa
    11221 tctccatacc tataataatt ggcatggcac ttatggcatc gggtattcag catctggtga
    11281 tggccttctt cgggccgcgc gatctggtct aaaacagaaa tcaacgcggt tcgccatttt
    11341 ccttaaaaaa ttcgggtgcg aaacttcgca cccgggttta tttcatggat agttgttatt
    11401 atcgttcaga gaagttcctt caaaacatca ccgatcttca gggcttcgtc caccttaatc
    11461 atcggcgttc ctttctcctc ggacagtttc ttgaagtaag aactgacctc atcgggagtc
    11521 accaaaagca caagatctac gtacggcatt atcgcgtcga tcgatctgtt gaaattacgt
    11581 tcggcaatat cgattccccc ctctatatgc agacccacaa tcgacaatcc cgaagctctg
    11641 gccttttcta tgagtttctc tattctctcc agctctcttt ctatatcgat ttcaatcgct
    11701 ccgcatacag tgcataagtc gccctcggtc attgcgcccg tggcgatgac cagcgtcttc
    11761 ggtccgttgc tttgttcgag cctttcgccc agagtttctg cggtcatatc cagcacgttt
    11821 acgcattcga tgccatacct catacaaacg agttcgagaa cgatagaacc agaaccatca
    11881 cccataacgg ttgccaccat aggagttttc actatcgttg tagaatcggc tactccggcc
    11941 agaactacgc cggaaatcat aagcacggcc agcaaaaatc cagaaatctt tctcttcatg
    12001 cctttccctc caaagtaaaa attggcgacg ctcaacctga aaacatgact gtacgacccg
    12061 gttgatttca taattgcgcg agtcgtatct agacatattt tatcagatat tataccgcat
    12121 aatactctgg agttcactcc agatggatat cgaaataaat ttccggtgga aggttaaagc
    12181 gatagctagt ttttcgatgc atcctgtttg aagtcgaagc cattctctat caaaccgatg
    12241 cagacctcta cgatcctgga atcgaaggcg gtgcccgatt taccgcgaat ctcttccagc
    12301 gccgtctcca tcctgtgggc cggcctgtac ggcctgtgag aggtcatcgc ttcaactacg
    12361 tccgcaacgg cgataattct ggcctcgacg catatttcat ttcccttgag cttaccggga
    12421 tagccggagc cgtcgagtct ttcgtggtgc tggtgaatta tttccgcgac tggccaggga
    12481 aggcgtgtgt ttctgaggat attgtatcct acgatgctgt gctgctgaat caggctgaac
    12541 tctttgtcgt tcaacttgcc gggtttagag aggatttcgg cgggtatggc tatctttcct
    12601 atatcgtgga gcagtccggc gactttaacg gcctcaatcc tggtgtcgtc caggccgatt
    12661 ttctctgcta tcttcgagga taaccggctc acattgcgat gatggtattc gatataagga
    12721 tctctcgccg tcagaatatc ggaaaccacg gttattattg agtaaaaggc ctctctgatt
    12781 ctttcggcgg cctcatgcct ttccgtcaca tcacgggcta tgccttcgag ggcgatcagc
    12841 ttctcgttgt cgtcgaaaat gcggacattc tgctgctcga tccagacgac ctttccatcc
    12901 tttctcaccc atctcatagt caatgtttct ctctgttcgg agtgagtgat gtaatagttc
    12961 gtaatattca gaagcctgtc tctatcttcg gaatggacta ttctcgttat cagatcggga
    13021 tccttgtaga agttgtctgg agtgtagccc gtgatgcgga cagatgccgg actgacatag
    13081 ctgaaccctt cttctggata gagttcgtat ctgtaaatca gatcgttggc cttctctgcc
    13141 agcagcctga acttcttctc gctctcgatc aaagcctttt cggctttctc tttggctatt
    13201 ttgatctcct ttttctccag tgcctcgacg actgcatacg gtagcctggc catgtgctcc
    13261 tttatcacat aatcgatcgc tccggccttc atgcattcgg cggcggtgtc ttcgttcatg
    13321 gagcctgtca ggattatcac cgggagctcc ggagcgtttt tgagaaccag ctctataacc
    13381 ctcatcccgt cgaagcccgg cataagatag tcggagatta cgaggtcggg cgagaactcc
    13441 ttcagctttt ccatgaaatc gtgttccgta tccaccacag cggcctcgat ttccggaaag
    13501 actctggaga tctcccatct ggcaagatcg acgtcagtcg gcagatcttc cgcgaaaagg
    13561 atttttatct tacctttttt actcatcttc ggactcacct cgatggaagc ctgttaagta
    13621 ccagccagta atcgttcatc tgtctcagtg actgaatgaa attctcaaag ttgacgggct
    13681 tcacgatata actgttcgcc cccagtttgt acgattcttc gatatctact tcctgggtgg
    13741 aagaggtgag aatgacgacc ggtataatac tggtcctggg gtctttcttt atctctctaa
    13801 gaacctctat tccgcttact ttaggaagtt tcaaatccag taaaatcacc tttggagagc
    13861 aaatgacgct acgatcactg tatttgccgc gggcaaatat aaaatccaga gcctcctcgc
    13921 catcttcggc gaccacgata ttgtttgcca gtttgctctt tttgagagct ctcagtgtga
    13981 gttcgatatc ctcgggattg tcttccacca gaagtatctc gactacgcta tcgatcatct
    14041 ctttcacctc taggggatgg aaaagtagaa ggtcgctcct tgattctctt cgccttcggc
    14101 ccttacggaa cctccatgcc tgtgaattat cctctgaact atcgaaaggc caacgcccgt
    14161 cccttcgaac tctttggagc tgtgaagcct ctggaagatt tcgaagacct tgttcgcata
    14221 tttctggttg aatccgacgc cgttgtcctt cacgtagtac gtgttggttc cgttctctga
    14281 ataaccaccg acctctatgg ttataacctc cctggtagcc gagaacttca aggcgtttga
    14341 aagcaggttt atccacacct gtttcatcat gacgctgtct ccatagacct ccggaaggtt
    14401 ctgcaccgtg aatttgactt tctcgcgaag ttcgacagtc accacttcat taaaaagcga
    14461 atttaccatg gcgatcatgt ctatcttcga cttcaccagt tcggtcctgt tcactctgga
    14521 cagagccagt agatctagaa tcaactggtc catcttcttt atgttgcgct ttattacccc
    14581 gaagagcctc aacccttcgc tgtctagcac gtttacataa tcttcctcga ttattctcga
    14641 atacccgtcc acggcccgca aaggggcccg caggtcgtgg gaaaccgaat aggcgaaggc
    14701 ctcgagttcc ttgttgacgg cctcgagctg tgtggtcctt tcggcaactc ttgcctcgag
    14761 ctcgacgttc aatcgctcta ctttttcctg gtaagtgcgc atgtcggtga tatcgtggac
    14821 gacctgcagc ttcgatatgc ttccgtcgtc gttgaaaaca ggtatgtcga tcaggtcgta
    14881 gatacggcca cttctttcgg accttcgttc ccagcggacc ggttggccgg aatggatctg
    14941 atcgtttttg caccaggggc agggagcatc gaactttaga aagaattcat agcacttctt
    15001 accttcgata gggccgtact tttctatgag aacgggattt acatactcga tatcgtagct
    15061 ctcgttgacc atatagatgc cttcgggtat atagtccagt atctgacgga gtttctttct
    15121 ttcgatctcg agttttcttt cggcgttctt ctgctcggtt atatccctgg cgaaaaccga
    15181 tatcccttca cccagaggat agatgtttat ctccagtatc gaggtctttc tcatgctttt
    15241 gtattcgaaa accgaagatt ccggcttccc gctctggaga acccttcggt aggaggccat
    15301 ggcttttgca gtggtggcgt cttcggggaa gacatccgtt acgtgctttc cgataacgtc
    15361 tatggacttg agcccggtca tcacctcgct ggcgctgttc cagtatatgt acctcaactc
    15421 tttatcgaga gcataaagca tgtccgttac gcttccggta accttcgcaa ggagttcttt
    15481 agtctcgagt tgctgccttt ccagagtcct tatttgagtg atatcgaagg cgtagatcct
    15541 ggctatatcg cctccgggaa gaggattcaa cgatacgaag aagatcttct ctcctatagt
    15601 cttttcataa ttgaaagggg aaggttcttt cgacttccag atctcctgtg gaagctcttc
    15661 tggtagaaat gcaaccgggt ctttcaatcc actcgaattc aaaaaagaag tagccgcagc
    15721 gttctggtag gttgtttttc cgtcgatgtc gatgtcgagg atagggttca ggttcctttc
    15781 gacctccgta gaagggcgga agtcacgctc catcggatca aggctcgatc tttcgtgact
    15841 cataagaacc ccctggttat caaacgagat ggattgattt ctacaaccga caaaaagatt
    15901 atagcatgtt gcaaattttc gaaatcacat tcgagagtat atttccaggc ttttcaaact
    15961 tccatataga aaactacata gacatctcgt agatgttccg cagatcctcg gttttcaggt
    16021 ttcccggagt tacgcttacg agccctccca tcgttctcat ggcgtcttcg gccaggattg
    16081 aaagtttgga gctctccact cccagttcgc cgagggaaat atcgagaccg acattcttct
    16141 gaagcctgcg gagcaagact atggccatct tcgaaagctc ttccacgcct atgacgcttt
    16201 cgggcgctcc cataaccctt gcaacttctg ccagtctctc cgcgatatgc ggacggatgt
    16261 attcgagcag cgaggggcct gtagcacaga gtccggcgcc atgtgtcgcg ttgtagtggc
    16321 cgctaacggg gtgttccagc gagtggttgg ccacacaacc cgaaagcgtc tcgcatatgc
    16381 cggccgccgt gctcgcccac gcgagggcta ctcttgcgtc caggtcgttc gggttttcgt
    16441 acgctatagg cagataatgg tttatgagcg acatcgattc cagagccaga agatcggagg
    16501 ccggctgatg attcaaattg atatatgatt ctattgaatg atagaaggca tccatgccag
    16561 tcgctgccgt ttgttcggga ggaagcgaca acatcagctc gggatcgact atcgaaacgg
    16621 ccggaaagat ctcgtcgaaa cccacgccga tcttctcgtt ggtctccggg ttggtgacca
    16681 ccgcgaaggg gtcggcctcg gttcccgttc cgtgggtcgt ggggatcgct attacgggca
    16741 aggccgcttt cggctttctg ccgccgccgg caccggtgta atcccagcac tttcctccgt
    16801 ccttcgccac gagggcaatc aacttggagg agtcgatcgg gctgccaccg cccaatccta
    16861 tgatgaaatc gcatctctcc ttgacggcta ttgaagctcc tctgtcgata ctggtagaga
    16921 gagggttggg tactatctcg tcgaacacta ccgaggagat cccttcctct ttcaacagcc
    16981 cgatcacttt atccagcaga ccggtcttct tcgtactgtg ccttcccgta actataagcg
    17041 ctttcttgcc cagtctcctg gaatgctgtc ctaccttgcc gatagttccg cagccgaaga
    17101 ttactcttgt gggaagaaaa taatcgaaag tttctatgct cacgaagtgc gtgcatgtaa
    17161 cagctaatag aaaggagcaa gagaaggaga gcggaaacag gaatcgagga gatgaaagtc
    17221 gattttgacc tgttcgagga gtaaagacct gagcatagag attgctcttt ggattgtttc
    17281 gaacacttta tacctctttg tttccgactt agtcttctcg agaatgaatc gctgaaggtc
    17341 attgaaaatc agataggcaa gaataaccag gataatccaa tgcttgtggc catcttcagt
    17401 cgagagtccg gctttaccga gcgagaagtg ttgtttggca gtcttgaagt aggactctat
    17461 cgaccatctg tggagataat acagaaagat atcctcagat gaaagctccg tatctgaaga
    17521 gatcagggtg gttcttctcc catcatccag ctcggccaca actatcttga gaggaactct
    17581 aacagacttg aagcgaacaa ctctctggtg gaatctgacg gttctatctc ttatgacaac
    17641 ctcctctgag aaatcataaa cagagttctg gtaatgggat actcggatga tagagtcctc
    17701 tgtcttgaat aagagatttc ccctgacttt tccaatgaaa gtgatcccga accagagttt
    17761 gcaggactct atcacatggc tgttggtgta ccaggagtct ccaaggagta ctgcatccct
    17821 tatctcgttc tctttcagcc aatctataag tctttcaatc ttggtgggac ctccattcga
    17881 gttcgagaac atcatctcca gaggaaagaa accatcgctg actctaccaa cggtaaggag
    17941 aacctgtgcc tggcaataac gcttctgatt gtgatcgtac gtcggagtgg tgaaaggtat
    18001 cttcttactc cttctggcca gttgagtatc atccacaacc aggatcatct tctccgaaga
    18061 ggttgagaga ataacctttt ggatcagctt ctcaaagttc ttccagaagt tgggatgacc
    18121 gttgacaaac ctggtaaggg ttgtatgagc caccttgttc aaagggtctc tagctattga
    18181 ggagatcgaa tgatggccaa gctccagtat tccctgtata agaatcagga tgtccttgag
    18241 atagaaggcc tctattgaag agaagaaatc accaaagcgt agctggacaa aatgctcaat
    18301 tgatgcaaga atattcataa ccggtcgctc ctttgtgtgg tttgtttgga caaaatattc
    18361 tacatctgga gcgtccggtt ttctattctt cccttctcta ttcccttttc aatgaccttt
    18421 ttcctttgtc ttgcctctct gattcatttc tccgttactt tcacgcactt cgtgagtaga
    18481 gttacttata agctataata gcatatatcc cattgctatt agacccatat a
//
LOCUS       DAOOLY010000012        18433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3102_length_18433_cov_4.921101, whole genome shotgun sequence.
ACCESSION   DAOOLY010000012 DAOOLY010000000
VERSION     DAOOLY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 18433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18433
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_18433_cov_4.921101"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLD15_01280"
     CDS             complement(<1..831)
                     /locus_tag="PLD15_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62558.1"
                     /translation="MLIQILSILVGVATFAIVVSMIFNPVIFKIGFRNIFRRKADTLL
                     VILGSLIGTALIVGSMAMNDSFQKFLHNQIERSNGEIDEFIFIPSDNQTIVKEFIPNG
                     EIETLVKHLLQDSNVDGLLPLLSRNVTVGRPGETRAQNGKSVQISLIGVKPEDLTKWP
                     DSKGVVDPSDLSSTDGIASVVINKELADSLALSVGDTLEIMSDAGQRLLFWIELPKVQ
                     ISDIVKGDGLLYYQVEANNQPPNGFTMLADIDTARDLLKINVPDYYNGLLVSNRGDFL
                     G"
     gene            complement(812..1534)
                     /locus_tag="PLD15_01285"
     CDS             complement(812..1534)
                     /locus_tag="PLD15_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62559.1"
                     /translation="MTFVEVRDLSKTYRSGEVVVEALKEVSFDIYEGEILSILGPSGC
                     GKSTLLNCLSGIDEPTGGKVIIKGIDLHSLRDDEKTRFRAMNMGFVFQFYNLIPVLRS
                     VENVELAMLTMGSSQKKARQAAMDILGKVNLDGRATYLPSQLSGGERQRVSIARALVH
                     RPAIVWADEPTGALDTKTSTELMNLIIELNKTFNQTFVIVTHDERVSNYSNRILHMDS
                     GKILKIDENQNLKVSQNVDSNS"
     gene            complement(1577..2056)
                     /locus_tag="PLD15_01290"
     CDS             complement(1577..2056)
                     /locus_tag="PLD15_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62560.1"
                     /translation="MDLVFFFLPAVVLLDYYIVLGLPGSSFAGRVALFVQKADSLLNF
                     RKTFEEETKGKLIDSENLKNLEQVVASLESRLRKPAEIQRKLYLFSIYVAPLFPMAVM
                     LSSILLQRRTELYAGLFSYGASLIIVILARRAFRTLENTIEKLNNEIRKAIEDISYN"
     gene            complement(2130..2750)
                     /locus_tag="PLD15_01295"
     CDS             complement(2130..2750)
                     /locus_tag="PLD15_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPB62561.1"
                     /translation="MENRIKWGARAVSSDGKDLGKVIRVVVHPKNNEVTHVVIEKGIF
                     NRFAKLIPIATVFFAAPDEVRLKIDSKNVESLQDYEETFFVTGEEIENLESGVTPVYW
                     LRPVGDYAELYPLPPLNTSINVPKDTKSLEPGCDIVTVEDKAIGRVRSFVLNDEGKIT
                     HVVGDIGGFGSKSRKLIPIDWVEDIDEAKVLVSASAIMIEKLPEME"
     gene            2952..3485
                     /locus_tag="PLD15_01300"
     CDS             2952..3485
                     /locus_tag="PLD15_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPB62562.1"
                     /translation="MSLFKLFMIFVKVSSFTIGGGYAMVPVIKEFIVDKYKLLGEDEF
                     MDVIVTAQTVPGVIAVNTAMILGTKLAGLWGAVLAVLGSILPPFLIILVIASFFADFA
                     DLPVFEGFFMGARVGVTVILANLSLQLIKKNVRKYLILAIIAAGTAAIVLLNFSSIWI
                     LLISSVCVYFIDRGRKR"
     gene            3482..4012
                     /locus_tag="PLD15_01305"
     CDS             3482..4012
                     /locus_tag="PLD15_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPB62563.1"
                     /translation="MIFLELFWAFFQIGFLAFGGGYGALSLIQDQIVNVNKWLELPEF
                     LDLISISQMTPGPIAINSATFIGYRIAGLGGSIISTIGVVLPSVFWIFLILKLLKILS
                     RWIDTAQVFNALRVSIVSLILAATFRIGIESINNLFTVVIGLIAFYVLYRYKPSVIWI
                     VLGTGVAGIFWRLIAG"
     gene            4603..4749
                     /locus_tag="PLD15_01310"
     CDS             4603..4749
                     /locus_tag="PLD15_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62564.1"
                     /translation="MKKLIAILLIGMVAFGTVAFAGPGFWKIPEDAITADGGPGFWKI
                     PEGA"
     gene            4820..4966
                     /locus_tag="PLD15_01315"
     CDS             4820..4966
                     /locus_tag="PLD15_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62565.1"
                     /translation="MKKMIAILLIGMVAFGTVAFAGPGFWKIPEDAITADGGPGFWKI
                     PEGA"
     gene            complement(5011..6726)
                     /locus_tag="PLD15_01320"
     CDS             complement(5011..6726)
                     /locus_tag="PLD15_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB62566.1"
                     /translation="MKNFRIRDLALRFNYDSPSIFRSEVKAIDRDEFLSAEEREFLLA
                     LDFAYAKTFDEKPGLVIQDSDIDSLAKGLYALSYFYARNSEDKIAYMVSRLGVDLIAP
                     KCSAENSINLRMLHMMTSFEMGFAKETLSLFHQLIGQERFVPGTKKFEYYNNLGLVST
                     QLQTGDNPWELYEKAKAETRSPVRATIVQLNIADYLYSQKRFKEALNALEETIEIPDF
                     DSVNGYRLMLNLKIMLQMNDLANAGRVAEQLEALIHSSSSWTDIAVSYIFLGHFFSRV
                     KSIEKARYYLNLLKSLPDECVTNYILGETLILEASLLHNRGDYFKALENSVRAFESLS
                     VYSTISPHLKDFVNNLFESLVSVFNQLMYELRRKDDYTALHTLRVLKLCYGFGKSLSL
                     EKMDLFNLSIGAMLHDYGKVDIPFEILNKPSLLTDEEYSIIKCHTIYGDKYLGDLRFP
                     GTVRDIVKHHHERIDGNGYPDNLMGDEIHYLVQIVAIADVYDALTTDRPYRKALSRET
                     TLSYLKEKGGQIIEIGLLRKFIAYVRMNEIIVKHEELENIWNTIISEFFRVTDINETA
                     DMQGQ"
     gene            complement(6985..7581)
                     /locus_tag="PLD15_01325"
     CDS             complement(6985..7581)
                     /locus_tag="PLD15_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPB62567.1"
                     /translation="MRVRKQQSFPVFRIIFISSLAVSLVLLVLFLMLLLKTGRLEKTL
                     QALEKRYSALELELGAVTGTQRTMKISLYYYNELLDRAINGDTACDINAVIPVERSLP
                     YSQSPINDVIRLLIRGELTRAERDLGFKTEFPGRELQFLGARLENGVLYLKFSDPSGF
                     TSGGSCRIGLLKAQIEKTVMQFDTVKSVVYEPETLFQP"
     gene            7854..9092
                     /locus_tag="PLD15_01330"
     CDS             7854..9092
                     /locus_tag="PLD15_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPB62568.1"
                     /translation="MRQEPLKRNDQHKVQAFHVMAKPTGALCNLDCAYCFYLHKQELI
                     NSRKVIGEEVLERFIYEYINSQNVSEIVFTWQGGEPTLLGIDFFKKVIEYERLYCPPG
                     KKIANDLQTNGTLLNEKWCEFLKEYNFLVGISIDGPERLHNVYRVSKDGGPTFKRVFN
                     ATKLLHRYSIDFNTLTVVNRINAKFPGEVYRFLRDSVGSTRMQFIPLVEPKIFKERPT
                     TFIAESSMPIIGTNEALPAKAGSIATDWSVVPQDYGRFLNGVFDIWYRNDIGRVFVFL
                     FECALGQWLGMQSSLCIFAEICGRALAIEADGSIYSCDHFVYPEHLLGNIMNKRLIDM
                     VNSTSQTVFGISKAATLPDYCHRCEYLFACHGECPKNRFVRTLEGEAGLNYLCSGLKL
                     YFSHIDPYMKELAEAFRSGR"
     gene            complement(9149..10129)
                     /locus_tag="PLD15_01335"
     CDS             complement(9149..10129)
                     /locus_tag="PLD15_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB62569.1"
                     /translation="MKIIESFGKEELAIVHAGETSRASWVEFVESLQPPLPREEKWVL
                     IVSTMDGCPVQCAFCDAGGAYRRNLSSEEILEQIDYMVSRRYGSTVNVKKFKIQFARI
                     GEPALNPSVLEALEMLPQKYDAPGLLPSISSIAPASSKGFFEKLYTIKERLYRGRFQL
                     QFSIHSTDIAQRDWLIPVRKWSFEEIADYGAYFCGPTDRKITLNFALSTMSLVDSDII
                     SRFFDPRKFLVKITPVNPTYRSHEKEIKTAVEDDGSLLLHASFVDGLREKGFEVIVSV
                     GEPEENRIGSNCGLYIRRHIDRMKESPEMYSFVKSKNDPERSDSDHGVPK"
     gene            complement(10136..10927)
                     /locus_tag="PLD15_01340"
     CDS             complement(10136..10927)
                     /locus_tag="PLD15_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB62570.1"
                     /translation="MINTVGVVGAGPAGVAAAIMLKRYGIEVLIFERRTVGGLLNNAW
                     RVENFPPLEPCTGEELCRKLEDRLNVNDIAIIPEEVTAVRDRIIVTRRARYTVDYTLV
                     ATGTAPKRLPSLETCSRVVYEYRDIPPGTEVVAIYGAGDMAFDGAVRASLRGSRTLLF
                     ARGETLRAIEALKVSAEKAGVELHRAEPILRTEPDEALLKITTALNVYSVDALLICIG
                     RESLLPRIDSDRCEIIGDARGEIYRQASIAIGDGIKSAMKIALEG"
     gene            complement(10924..11517)
                     /locus_tag="PLD15_01345"
     CDS             complement(10924..11517)
                     /locus_tag="PLD15_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPB62571.1"
                     /translation="MLTEALFEGTFGRERQKHRPVLLSPALLVIDLQNYFSDRKSLAY
                     LEGIEKVVASVGRLVEGFVSCRLPVASTVHRGGSKMMEEWWGNTVADRWAIPQFSDFP
                     VFYKDSYDAFQGTALDAFLIDRGVNQLVICGVRTHLCCETTARSAFVKDYGTVMVEDA
                     LCDKDVDQHVRSLKNLASGFSAVSNTPEILDLLRERL"
     gene            complement(11797..13086)
                     /locus_tag="PLD15_01350"
     CDS             complement(11797..13086)
                     /locus_tag="PLD15_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB62572.1"
                     /translation="MKGSKKGFVFVLILVFFSSMMISASLTREIEPLPLWEKESAHEA
                     QRIIVVSDLHLGVDDSFSETVKNKDLLTEFLERLVISDIDELVIAGDMLDEWFVPISY
                     EPHNDLGAFFEKVAENNASIVAAFEKIIQSGVVVAYVPGNHDLLLDEETLAKLIPGIV
                     QARDVDGLGTYRTGIRSEIVIEHGHRYDTFCAPDILSNKEMTGDYPSFLPPGYFFTRI
                     ASTSVVEGKSAPDKNLPRIEAPSKEDADQLDAYTYYNIWLWAMTTFPIKADFDEKAIY
                     VGVNGYDNSFSLSDLLPTVQDDGSISAVLYANAQRRWDEVQQNNRVAVKNPYSKATAG
                     AIDHTFFDEQAVKQYFDLDPTVDVVVFGHSHVPLVNRYENEYDKEKVYANSGTWIDEN
                     LIGLERCFVVIESGKQFTDVRLMEYHADGTISNTEQK"
     gene            complement(13595..14113)
                     /locus_tag="PLD15_01355"
     CDS             complement(13595..14113)
                     /locus_tag="PLD15_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPB62573.1"
                     /translation="MIFMTRRNNGFSLVELLIVLAVMAALISTITPVAMNAIKKAKAT
                     QVAQNLKTLSSALQHAAYLNGANSDGQIKSHGNTAIELNDLGRDLPKDSNGNDLYGFA
                     YTRTSGGTYYAVVYYAGGDVDLATAGEILGNQSLEYTSTHLDADDSLVEDGATYQSSA
                     NDVYYYFYFTIY"
     gene            complement(14263..15537)
                     /locus_tag="PLD15_01360"
     CDS             complement(14263..15537)
                     /locus_tag="PLD15_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB62574.1"
                     /translation="MTIKNKITLLYLATYGSVVFALSITLFMIFRTLEYRRIDGLLLS
                     FHNDIVNTYKYAGEGEKRLLSLAGDENLGFALYAGEKPLASFRIEPGTLSSVGPGMGT
                     AGDYRYRATLESIEGGDRKVVTFYRLEGTRAHLNNLLLIIILSAAFTLLLVSLVGMAF
                     TRKLMKPLEQAGNQLERISRSEIGGARVEVENTGREIEKLQLEINRALDRIERLVEDT
                     RQMSSKIAHELRTPLAVMKTNLQLSLEKNSSPGEMRLSLKDTMKELDKLIRMSEGFLL
                     LSRIESAVPLEMARVDLSGLLLETIEKIMILYPGVEFALDVAPAIEMRGVSHMIEHAI
                     INLLDNAARYTTDGSVSVRLSTGERYIVLEVTNRGRKVEMERPGMLSGSSVAAGNGIG
                     LTVVQAVTKLHKADLLYEHRDGTNRFMIRFPA"
     gene            complement(15543..16220)
                     /locus_tag="PLD15_01365"
     CDS             complement(15543..16220)
                     /locus_tag="PLD15_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB62575.1"
                     /translation="MKLLIIEDERKLSDNISDFLKGEGYSVDAAFDGEEGLDLALERS
                     YDCIILDILLPGMDGLSLCRFLREEIKSTVPILMLTALGELDNRIEGLNAGADDYLPK
                     PFDLHELLARVKALLRRAQLRGGEKIACGELVLDSRKQTVLYREKPIELSAREFSILE
                     LLMRNPGTVFTREEITDKIWESGYEPRSNIVDVYIRYIRKKLEALKLDHIIETVQGRG
                     YRLREKP"
     gene            complement(16226..16897)
                     /locus_tag="PLD15_01370"
     CDS             complement(16226..16897)
                     /locus_tag="PLD15_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62576.1"
                     /translation="MRKVIRALAVSVLIFLSISVPGQTTLLEAEQELLSQLLEGGEPS
                     RFLDLELIDLAVKKAGGNVRALYYLPSEGTLTVITDRNSLTLTPDLEITADDRFETPL
                     AGVNVKLSLIDAFSLVSLQTREGILGLYLETAESGPLWHVITESSAYTLGAAFASTVS
                     VSDTRSALNAVSEKIKKEKAKAEKPGQPEEPGQSDQPGKPDEPGRPDDPGKSDDTPGK
                     GNGKK"
     gene            complement(16894..17766)
                     /locus_tag="PLD15_01375"
     CDS             complement(16894..17766)
                     /locus_tag="PLD15_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HPB62577.1"
                     /translation="MKKTNFFLLVVLTIFLISGCFNFPVPKPDENGTLRVSLTDAVIP
                     LEDIEKLEVTIESMALYGADGATPTEQTPLIVLDEAITVDIMTLIGEEIDLGEFEASG
                     IYNQLRMEISEATLTANGKVFPVTVNSGSLKINNLDIDLSKDEDTVLVLDFDLSRSLK
                     INGKWEETKGGKEGHEDKVHMTPVIHIRHGSLYDVTGVATPTGEASPLLVALVPDGTG
                     DTLLTFTHTNTPKWEKGEFRFCKVAPGSYTLKFFDNYTDEGFDVDTSVPLYTYPEPVV
                     VVDRDLSLGTIVLE"
     gene            17971..>18433
                     /locus_tag="PLD15_01380"
     CDS             17971..>18433
                     /locus_tag="PLD15_01380"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPB62578.1"
                     /translation="MKIGIPNALLYYYYGPFWTEFFKELGAEVVVSHSTDKKTVDMGV
                     GISVPEICVPIKIFNGHVLQLLKGGVDYVFIPRMASVEKGKYFCPKFMGLPDMARHSI
                     PGVAGKLLTMDVQSRSENIFSPKMYYSMAEKLSVSDKQIELACRKGAELWRK"
BASE COUNT         4434 a         4628 c         4529 g         4842 t
ORIGIN      
        1 gccaagaaaa tcccccctgt tggataccag aaggccgttg taataatcgg gaacatttat
       61 tttgagcaaa tctctcgcgg tgtctatatc ggcaagcatt gtgaacccgt ttggaggctg
      121 gttgttcgcc tcaacctggt agtaaagcag accgtcgcct ttgactatgt cggagatctg
      181 gactttgggg agttctatcc agaaaagcaa cctctgtccc gcatcggaca ttatctccag
      241 cgtatcgccc accgatagag caagcgaatc ggccagttct ttgtttatga cgacactggc
      301 gatgccgtcg gtactcgaga gatctgacgg atcgaccacg cctttcgagt ccggccattt
      361 agtcaggtct tcgggtttaa caccgattag tgagatctgg actgattttc cgttctgcgc
      421 tctcgtctct cccggccggc ctaccgtgac gtttctactc aacagtggga gaaggccatc
      481 gacattcgaa tcctgaagca gatgcttcac cagagtttcg atctctccgt tgggtatgaa
      541 ctctttcact atggtctggt tatccgaagg aatgaagatg aactcgtcaa tctcgccgtt
      601 gctccgctct atctggttgt gcagaaattt ctggaaggaa tcgttcatcg ccatcgagcc
      661 gactataagg gccgtaccta taagcgagcc tagaatcacc agcagcgtgt cggcctttct
      721 tctgaagatg tttctgaagc caatcttgaa tatcaccgga ttgaagatca tggagactac
      781 tatcgcgaag gtcgcaactc caaccaggat actaagaatt tgaatcaaca ttctgagaca
      841 ccttcaaatt ttggttttcg tctatcttca atatttttcc gctatccata tgcagaatac
      901 ggttagagta gttcgacacc ctttcgtcgt gtgtaacgat aacgaaggtc tggttgaagg
      961 ttttgttcag ctcaatgatg aggttcatga gctcggtgct ggttttcgtg tccagagccc
     1021 cggtaggttc gtcggcccag actatggccg gcctgtgtac tagcgccctg gctatggaga
     1081 ctctctgcct ctctcctccg ctcagttgag agggtagata tgtcgcccga ccatcgagat
     1141 tgactttccc cagtatgtcc atagcggcct gtctggcctt tttttgactg cttcccatcg
     1201 tcagcatagc cagctcgacg ttttcgaccg atctcagaac tgggatgagg ttgtagaact
     1261 ggaaaacgaa acccatgttc atcgccctga accgcgtttt ttcgtcgtct ctcagggagt
     1321 ggagatcgat gccttttatt atcacctttc cgccggttgg ttcatctata cccgacaaac
     1381 agttgagaag agtgctcttt ccacagcccg aaggtccgag gatcgaaaga atttcaccct
     1441 cgtatatatc aaacgaaact tccttcaatg cttcaactac gacttcgccg gacctgtaag
     1501 ttttgctcag gtccctgact tcgacaaacg tcattggtag catctccttt cgatcgaaca
     1561 gccagaaaac aatgttctaa ttatacgata tatcttctat agcttttctt atctcgttgt
     1621 ttaacttttc tatagtattc tcaagcgttc taaaggctct tctggcaaga atgacgatta
     1681 tgagggaagc tccgtaagag aacagacctg cgtaaagctc tgttctcctt tgcagaagaa
     1741 tagaagaaag catcaccgcc atcgggaaga gcggggccac gtatatcgaa aagaggtaca
     1801 gctttctctg aatctcggcc ggctttctca accttgattc gagcgaagct acgacctgtt
     1861 cgaggttttt cagattttcc gaatctatca gctttccttt cgtctcttct tcgaaggtct
     1921 ttctgaagtt cagcaggctg tcggccttct gtacgaacag ggccaccctg ccggcgaagg
     1981 aactcccggg caggccgaga acgatatagt aatccagcaa gacaactgca ggaagaaaga
     2041 aaaacacgag atccacccgc acacccctgg cgaaattgag agcaaaaaga accactgaaa
     2101 aaaccatata ataaaccaac aaatcaagat cattccattt caggtagttt ttcgatcatt
     2161 atcgctgaag cggaaaccag aactttcgct tcgtctatat cttccaccca gtctatgggt
     2221 ataagttttc tggacttaga gccgaaacca ccgatatcgc ccacgacatg ggtgatcttt
     2281 ccttcgtcgt tcaggacaaa acttcgaacc cgtcctattg ctttatcttc aacagtgact
     2341 atatcacagc cgggttccag ggatttggtg tccttgggta cgtttatcga tgtgttgagg
     2401 ggaggaaggg gatacagttc ggcgtaatct ccgaccggtc tgagccagta aacaggggta
     2461 actccactct ccagattctc gatctcttcg cctgtaacga agaaagtctc ttcatagtcc
     2521 tgaagggatt ctacgttttt ggagtcgatc ttcaaacgca cttcgtcggg tgccgcgaag
     2581 aaaaccgtgg ctatcgggat caatttggcg aatctgttga atatgccttt ctcgataacg
     2641 acgtgagtca cttcgttgtt ctttgggtgt accacaaccc gtataacctt cccgaggtcc
     2701 tttccgtcgg aggaaaccgc cctggcgccc cactttatcc tgttctccat ttatctcacc
     2761 tcctttaaag acaagtttaa cacgcccggc acactctctg atgttgtttc agtgtataag
     2821 actgcaagaa aggacgccag gttgtaaaat atccttgatt ggccatgcac gtaaaggctc
     2881 cggagctccg gcgcgaccca tcttgataga gcggacagat acgacaaaaa attatgaggg
     2941 ttgacacttg tatgagttta ttcaagctgt ttatgatatt tgtgaaagtc agctccttca
     3001 ccataggcgg cggctacgcc atggtcccgg tgatcaagga gttcatcgta gataagtaca
     3061 aactcctcgg tgaagacgag ttcatggacg tgatcgtgac ggcccagaca gtgcccggag
     3121 tgatagccgt caacacggcc atgatcctcg gcacaaaact cgcgggtctg tggggagccg
     3181 tactggccgt tctgggttcc atattgcctc cattccttat aatactggtt atcgccagct
     3241 ttttcgccga tttcgccgac ctgccggtct tcgaagggtt tttcatggga gccagagtcg
     3301 gcgtaacggt tattctcgcg aatttatctc tacaacttat aaagaaaaat gtcaggaaat
     3361 atctgatcct cgcgataatc gcagccggta cggcagccat agttctgctg aacttctcct
     3421 cgatctggat actgctcata agctcggtct gtgtgtattt catcgacagg gggcgcaaaa
     3481 gatgatcttt ctagaactct tctgggcctt tttccagatc ggattcctcg cttttggcgg
     3541 aggttatggc gccctgagcc ttattcagga tcagatagtc aatgtgaata aatggctgga
     3601 gttaccggag tttctcgact tgatctctat ctcgcagatg acccccggac cgatagcgat
     3661 taactcggcc accttcatag gctacagaat agccgggctg ggcggttcga taatctcgac
     3721 gattggtgtc gtgcttccga gtgttttctg gatcttcttg attttaaaac ttctcaaaat
     3781 actctctcgc tggatagata cggcccaggt cttcaacgcc ctgagggttt cgatcgtttc
     3841 gctcatactg gcggccactt tccgcatagg gatcgaatcg atcaacaacc tcttcaccgt
     3901 ggtgatcggg ctgatcgcat tctatgtgct ttaccgctac aaaccctccg tgatctggat
     3961 agttctgggc acaggagtgg ccggtatctt ctggagactt attgccggat agaatctgga
     4021 atcaaggtac atcgactcct attttttaca taatgccttt ccgtagtttt gaacaattca
     4081 aactggattg aatagttaga tgatagtgtt cgaatcaatt caacgattgt tttttttcga
     4141 aaacacgcgt gaatcgacca cggccatcga taagtataat ataattcaat agattaaatc
     4201 gaattagata ctctattgat taatgtgatt ttccatagtc gatgtatgac gattatggac
     4261 attcaaagct atttaatacc gattatcaaa tgaaatctct ttcttgctaa tctttgaatt
     4321 atcttacgat aacatctaaa atgtacttga tatccaaata tatagtgaag agcacggcga
     4381 taggttatct caagcgatta gttctatagc aggtttttat tggcaattcc aaagattatt
     4441 tcaaaattat tcgatattac cgtagtgaaa tattactcaa acaactgaca aggaggcgtt
     4501 tttatgtgga agattcccga agaagaagaa ccgcattagc taccttcaaa ggcaggagca
     4561 gttcctggtg ttttgaaact actaaaatcg gaggtgtttg aagtgaaaaa gttgatagcg
     4621 attcttctca taggtatggt agcgttcgga acggtagctt ttgcgggtcc aggtttctgg
     4681 aagatccccg aagatgcgat aactgccgat ggtggccccg gtttctggaa gattccagag
     4741 ggcgcttgat tcgacagggc attcaatcat cgatgtttca atcagtgact attagaaggg
     4801 attaagggag gtctaagtga tgaaaaaaat gatagcaatt cttctcatag gtatggtagc
     4861 gttcggcacg gtagcttttg caggcccagg tttctggaaa attcccgaag acgcgataac
     4921 tgctgatggc ggccccggtt tctggaagat tcccgagggt gcttgataag aaccggccga
     4981 cggcaggttc tttacggaac atctgccttc tcactgacct tgcatatcgg ccgtttcgtt
     5041 gatgtcagtc actctgaaaa attcggatat tattgtgttc catatatttt ctagctcctc
     5101 atgctttacg atgatttcgt tcattctcac atatgcgatg aatttcctca ggagaccgat
     5161 ttcgattatc tggcctcctt tttctttgag gtaggacaga gtcgtctccc gtgagagtgc
     5221 ttttctgtaa gggcggtcag tggtcaaagc gtcgtaaaca tcggctatcg caactatctg
     5281 aacgaggtag tggatctcgt ctcccataag gttgtcggga tagccgttcc cgtcgattct
     5341 ctcgtggtga tgcttgacta tatctctcac ggtaccaggg aaacggaggt cgcccagata
     5401 tttgtcaccg tagatcgtat gacacttgat tatcgaatac tcttcatctg tgagcagaga
     5461 aggcttattg agaatctcga acgggatatc gacctttccg tagtcgtgaa gcatcgcacc
     5521 tatcgagagg ttaaaaagat ccatcttttc cagagagaga ctctttccaa aaccatagca
     5581 gagcttcagg actctgagcg tgtggagagc ggtgtagtca tctttccgcc tgagttcgta
     5641 catgagctgg ttgaatacgg acaccagact ctcgaagagg ttgttgacga agtctttcag
     5701 gtgtggcgat atcgtgctgt aaacgctcag cgactcgaag gccctgactg agttttccag
     5761 ggctttgaaa tagtcgccgc ggttatgtag caacgaagct tcaagaataa gtgtttcacc
     5821 cagtatatag ttggtcacgc actcatctgg cagtgacttg agaagattta gataatacct
     5881 ggccttttcg atggatttca ctctcgaaaa gaaatgtcca agaaatatat acgaaaccgc
     5941 aatgtcggtc cagctactgc tcgagtgtat caatgcctcg agttgttcgg ccactctacc
     6001 ggcatttgcg agatcgttca tttgaagcat tatcttcaaa ttgagcatca atctatagcc
     6061 gttgacggag tcgaaatcgg ggatttcgat cgtctcttcc agagcgttta gcgcttcttt
     6121 gaatctcttt tgagagtaga gataatcggc tatgttcaac tgaacgatcg tagccctcac
     6181 tggcgagcgt gtttcggctt tggccttttc gtaaagttcc cacggattgt cgccggtctg
     6241 aagctgtgtg gaaaccagtc ccagattgtt gtaatactcg aacttcttcg ttccaggaac
     6301 gaaccgttcc tggcctatga gctggtgaaa gagagagagc gtctcttttg cgaagcccat
     6361 ttcgaaagaa gtcatcatat gaagcatcct caagttgata gagttctcag ccgaacactt
     6421 cggggcaatc aaatcgaccc ccagcctgga aaccatgtag gctattttat cctcggagtt
     6481 gcgcgcgtag aaataagaga gcgcataaag ccctttagcc agcgaatcta tgtccgaatc
     6541 ctgtatcaca agacctggct tttcatcgaa ggtcttcgcg taggcaaagt ccagagccag
     6601 gagaaactcc ctttcctcgg cagaaagaaa ctcgtcccga tctatggcct ttacctcaga
     6661 cctaaagatc gaagggctgt cataattgaa ccgcaaagcc aggtctctta ttctgaaatt
     6721 cttcaagatg caactcccca ttcccgaaat attctatcac gtcataagtc gactaatcgt
     6781 acgatcagag gccgaagata aaaacaaaac cccttccgga ccaccgaatc gacgctccgg
     6841 gtaaaatggc ttcaatactg agagatcagt tcgatgagcg caatcttaaa aaccactcta
     6901 aacgcaaaac cgataacccg gatagaccaa attgatcaaa aaaatgagag cctcaataca
     6961 gactatatat catcgctggc tcttctatgg ttgaaaaagc gtctcaggtt catatacgac
     7021 actcttaacc gtgtcgaact gcatcacggt cttctctatc tgcgccttca gaaggccgat
     7081 ccggcaggag ccgcccgagg tgaagccgga ggggtcggag aacttcaggt atagaacacc
     7141 gttttccaga cgtgccccga gaaactggag ctccctgccg ggaaactccg ttttaaaacc
     7201 cagatccctt tccgccctcg tcaattctcc ccttatcaaa agccttataa catcgtttat
     7261 cggtgattgg gaataaggaa gggatctctc aacgggaata accgcgttta tatcgcaggc
     7321 ggtatcgccg ttgatcgccc tgtccagaag ttcgttgtaa tagtaaagac tgatcttcat
     7381 cgtcctctgt gtacctgtca cggcaccgag ttcgagttca agagcggagt acctcttttc
     7441 aagagcctga agagtcttct caagcctgcc ggtcttaagc aaaagcatca gaaacaacac
     7501 caggaggact aaagagactg ccaggctgga aatgaagatg atcctgaaga ccggaaagga
     7561 ttgctgcttt ctaaccctca ttaaactcac ctctcaatca atgacacttc aattatacat
     7621 tttccgtcga tggaggatgc cccggaaccc aaattatgtg tttcgagcct cttaagagcc
     7681 atggtgagta gtggatagca tatttaggta cacttttcct gtattattgt accaaagaag
     7741 ctgagcccga tctaacttta aaggaatgaa tccaattgat tggcggttga gatctccaga
     7801 gttgacggga aggcttcgaa gctaccggag ttcaatcggc cggaggtaaa aatatgcgac
     7861 aggaaccgct caagagaaat gatcagcaca aagtgcaggc ctttcatgtc atggccaagc
     7921 caaccggggc gctgtgcaac ctcgattgcg cttactgctt ctacctccac aaacaggaat
     7981 tgatcaacag caggaaggtc atcggcgagg aagtactgga gaggttcatc tacgagtaca
     8041 taaactctca gaatgtttcg gagatagtct tcacctggca gggcggagaa cctacgctac
     8101 tcgggataga tttcttcaaa aaggtaatag agtacgaaag attgtactgt cctccgggaa
     8161 aaaagattgc caacgacctg caaaccaacg gaaccctgct gaacgagaaa tggtgcgagt
     8221 ttctcaaaga gtacaacttt ctggtcggca taagcatcga cggtcccgag cggctccaca
     8281 acgtgtatcg tgtctcgaaa gacgggggtc ccactttcaa gagagtcttc aacgccacaa
     8341 agcttcttca tagatacagc atagatttca acacgctcac ggttgtcaac aggatcaacg
     8401 caaaattccc gggtgaggtg tatcgctttc tgagggattc ggtgggttcg acgagaatgc
     8461 agttcattcc gctggtcgag ccgaagatct tcaaggagag gcccaccacc tttattgccg
     8521 agagttcgat gcctataatc ggcacgaacg aggctttgcc ggcaaaagcc gggtcgatcg
     8581 caacggactg gtcggtagtt ccacaggatt acgggcgctt cttgaacggt gtcttcgata
     8641 tttggtacag aaacgatatc ggccgtgtct ttgtcttcct tttcgagtgt gccctcggtc
     8701 agtggctggg tatgcaatct tcgctgtgca tcttcgccga gatctgcgga cgcgcgctgg
     8761 cgatagaggc cgacggaagt atctactcgt gcgaccactt cgtatatccc gaacacttgc
     8821 tgggaaacat aatgaacaag agactgatcg atatggtgaa ctcgacgtcg cagacggtat
     8881 tcggtatttc caaggcggcg acgcttcccg attactgtca cagatgcgaa tacttgtttg
     8941 cctgtcacgg agaatgtccg aaaaacaggt tcgtaaggac ccttgaaggg gaggccggac
     9001 ttaactacct ttgcagcgga ctgaaacttt acttcagtca catcgatccg tacatgaaag
     9061 aacttgccga ggccttcaga tctggccggt gaatacagac tgcaagagta tcttcagttt
     9121 tgtttaaggc tttaaacaac acaggacttc atttcggtac gccgtggtca ctgtccgatc
     9181 tttcgggatc attcttcgac tttacgaagg aatacatttc gggactttcc ttcattctgt
     9241 caatatgtct gcgaatgtac agcccgcaat tgctgcctat tcggttttct tcgggctcac
     9301 ccacgctcac tatgacctcg aaacccttct cgcgaagacc atctacaaag ctagcgtgca
     9361 agagtagcga gccgtcgtct tcaaccgccg tcttgatttc cttttcatgg gagcggtacg
     9421 tcggattcac gggcgttatc ttcacgagaa acttgcgcgg gtcgaaaaat ctcgaaatta
     9481 tgtcgctatc gaccagagac atggtagaaa gagcgaaatt caacgttatc ttcctgtcgg
     9541 tcggaccgca gaagtaagct ccgtagtcgg ctatctcttc gaagctccat tttcttacgg
     9601 gaatcagcca gtcgcgctgg gctatgtcgg tggagtgtat cgagaactgg agttgaaacc
     9661 ttcccctgta aagcctctct tttatcgtgt atagcttttc aaaaaagcct tttgagcttg
     9721 ccggagcgat actgctgatc gagggtaaga gacccggagc gtcgtacttt tggggaagca
     9781 tttcaagcgc ttccaggacg gaagggttta gcgcaggctc tccgattctc gcgaactgga
     9841 tcttgaattt ctttacgttg acggtggaac cgtatctgcg gctcaccatg taatctatct
     9901 gttccagtat ctcttcactg ctaagatttc tcctgtacgc cccgccggca tcgcaaaagg
     9961 cgcactgaac agggcagccg tccatcgtgg agacgatcag aacccacttc tcttcgcgtg
    10021 gaagcggtgg ctgcagagat tcgacgaact ccacccacga agcccgtgaa gtctctccgg
    10081 catgtacgat agccagttct tcctttccga aactctctat tatcttcatg attcatcacc
    10141 cttccagcgc tattttcatg gcgcttttta taccgtctcc gatagctatt gaagcctgcc
    10201 tgtagatctc gcccctggca tcgccgatta tctcgcatct gtccgaatcg atcctcggaa
    10261 gaagggactc tctaccaata catataagca aagcgtcaac cgaataaacg ttcagcgcgg
    10321 tcgtgatctt caataatgct tcgtctggtt cggtccgcag gataggttcg gccctgtgca
    10381 actcgacccc cgccttctcc gccgatactt tcaatgcctc tatcgccctt aatgtctcgc
    10441 ctctggcgaa aagcaaggtt ctacttcctc tcagacttgc tctgacggcg ccgtcgaagg
    10501 ccatatctcc ggcaccgtag atggcgacaa cttccgttcc cggggggata tccctgtatt
    10561 cgtaaaccac ccttgagcag gtctcgagcg agggaagcct cttcggcgcg gttccggtgg
    10621 ctactaaagt gtaatcgacc gtgtatcggg ccctgcgggt aacgatgatt ctatcgcgaa
    10681 cagccgtgac ctcttcggga attatcgcga tgtcgttgac gttgaggcga tcttccagct
    10741 ttcgacagag ctcctctccg gtgcatggtt cgagcggagg gaagttttct acacgccagg
    10801 cgttgttgag gagaccgccc acagttcgcc gctcgaaaat cagcacttcg atcccgtatc
    10861 ttttgagcat gatcgcggct gcgacaccgg ccggtccggc tccgacgact cccaccgtgt
    10921 tgatcataaa cgctccctaa gcaaatccaa aatctccgga gtgtttgaca cggccgaaaa
    10981 gccgctggca aggtttttaa gcgaccggac gtgctgatcg acatctttat cgcacagggc
    11041 atcttcgacc ataacagttc cgtaatcctt gacgaaggcc gatctcgccg tagtctcaca
    11101 gcacagatgc gttctcacgc cgcaaattac gagctggtta acccctctgt ctattaggaa
    11161 agcgtcaagc gcggttccct ggaaggcgtc gtaactatct ttgtagaaga cgggaaaatc
    11221 gctgaactga ggaatggccc agcgatcggc tacggtgttg ccccaccact cctccatcat
    11281 cttcgagcca cccctgtgaa cggtcgaagc taccggcagg cggcagctga cgaacccttc
    11341 tacgagtctg cccacactgg ccacaacctt ttcgattcct tccagatagg ccagcgattt
    11401 tctgtcactg aagtaatttt gaagatctat caccagcaag gccggggaga gaagcaccgg
    11461 tcggtgcttc tggcgttcgc gaccgaaagt cccttcgaac agagcctccg taagcacggt
    11521 ctcatctcct ctccgaagag tccgaattaa acaattctat cactatatga ccagattgga
    11581 aaagatatcc agtgtttgaa ggcggtgatg gttatgaaca aacgctcaca gttcttaaga
    11641 gtcgtggcgg gtggtgagtt gacgagtcca ctcttctgga cgaagtgtga ttcggaccta
    11701 atctggacac cgattgggca gcgaagaaac taaaagaaac cggctcccgc cactgtttca
    11761 gttgatgaaa gccggtttta tattagagca gggtccttac ttctgttcgg tattgcttat
    11821 cgttccatcc gcatgatact ccatcagacg aacgtctgtg aactgcttac cggattcgat
    11881 aaccacaaag cacctttcca gaccgatcag attttcgtca atccatgtac cgctgttggc
    11941 atatactttt tctttgtcat actcattttc gtagcgattg acgagcggca cgtgcgagtg
    12001 cccgaagacg accacatcaa cggtcggatc aagatcaaag tactgcttga cagcctgttc
    12061 gtcaaagaat gtatggtcaa ttgcaccagc agtcgctttg gaataaggat tcttaacggc
    12121 tactctgttg ttctgctgaa cttcgtccca acgtctttga gcgttagcat atagcacggc
    12181 gctgatcgag ccgtcatcct ggactgtagg caacaaatcg ctcagagaaa aactgttgtc
    12241 gtatccattt acgccgacat aaatggcttt ttcatcgaaa tcggctttga tgggaaaggt
    12301 tgtcatagcc cacagccata tattgtaata cgtgtaggca tcaagctgat ccgcatcttc
    12361 ttttgaagga gcttcaattc tgggtaggtt tttatcgggt gccgatttgc cttccacaac
    12421 ggatgtagat gcaattcggg tgaagaaata accaggaggt aagaatgagg gataatcgcc
    12481 ggtcatttct ttgttggaca aaatatcggg tgcgcagaaa gtatcataac gatgaccgtg
    12541 ctcgatgaca atctcggagc gtatacccgt acgataggtt cccagaccgt caacatcgcg
    12601 ggcctggaca attccgggaa tcaatttcgc aagcgtttct tcatccaaga gaaggtcgtg
    12661 attgcccgga acataagcta caacaactcc gctttggata atcttctcaa aagctgcaac
    12721 aatcgaagcg ttattttccg caaccttctc gaagaaagct cccagatcgt tatgaggctc
    12781 atacgatatc ggtacgaacc actcgtccag catatcgcct gcaatgacaa gctcgtcgat
    12841 gtcgcttatt accagtcgtt ccaggaactc agtgagcagg tctttgttct tcaccgtttc
    12901 ggaaaaactg tcatccactc ctagatgtaa atcactcaca acaattatcc tttgggcttc
    12961 atgagctgat tccttttccc ataaaggcag cggttcgatt tctcttgtca gactggcaga
    13021 gatcatcata gacgaaaaaa acaccaggat aagtacaaaa acgaatcctt ttttagatcc
    13081 tttcacagaa attcctccct atttgtcagg cttagagttc ttagagataa aagagaaagc
    13141 gcctatgaag cacaatcaat gccattaatc cattaagcgc ctccatcttt tgatataatc
    13201 tctcgtttat aacgattatt gtaatgcaaa aagaggccaa ttatgtgtca aatcaactct
    13261 gatttagcgg tggggtgtat attcttagga accatgatgg gtggtgagca gtgggtaaga
    13321 gcgggatgcc ggatcggagt ccggcatgac gggaagaaag aaggactgtc atcccgtaat
    13381 gctcctatca cgagaaggca agagcgaaaa cccctagaga aagaacatcg agagttcgct
    13441 tcgctcacga gaaagagaga attctcacat atctctctct tctatctctc tttctccact
    13501 ctctcttttt ctcgtgtcct tagcgagaac aaaattcctg gctccgcaac ggagaatcgc
    13561 tcttcccagg gacggttctt cccgctttta ccaatcagta gatcgtgaag tagaagtagt
    13621 agtaaacgtc gtttgcactg gattggtagg ttgcgccatc ttctaccaga gagtcgtcag
    13681 cgtcaagatg tgtggaagtg tattccagac tctggttgcc caggatttcg cccgccgttg
    13741 ccagatcgac gtcgccgccg gcgtaataca ccacggcgta gtaagttcca cccgaggttc
    13801 tggtgtaggc aaagccgtac aggtcattac cattggagtc ctttggaagg tctctgccga
    13861 ggtcgttcag ttctattgcg gtatttccgt ggctttttat ctgaccgtcg gagttggctc
    13921 cgttgagata tgccgcgtgc tgaagagcac tcgaaagcgt tttcagattt tgagctacct
    13981 gcgtcgcttt ggcctttttt atcgcgttca tcgctacggg cgtgatcgtc gatatcaacg
    14041 cggccatgac tgcgaggact atcagcagtt cgaccagaga gaaaccatta tttctgcggg
    14101 tcatgaaaat catttatacc ctcctgagat gtgtaggtca tcaaaatagt aaagtttatt
    14161 acttagatta tactttgaat cgaatggatg tggagagagt gggtgttaag agttttagaa
    14221 agcgaactga atcgagcgaa tcataccgta cggtcaagcc gatcaagcag gaaatctgat
    14281 catgaagcgg ttcgtcccgt cccggtgttc gtaaaggagg tcggccttgt gcagttttgt
    14341 caccgcctgt actactgtga gcccgatgcc gtttcccgct gctacggaag atccgctcag
    14401 cataccgggc ctttccattt cgacttttcg acccctgttt gtaacctcga gaactatgta
    14461 cctctctccg gttgaaagtc gcacactcac cgaaccatcg gtcgtgtacc ttgcggcgtt
    14521 gtcaaggagg tttatgatag cgtgttctat catgtgagag actccgcgca tctcgatggc
    14581 gggcgctacg tcgagtgcga actccactcc ggggtagagt atcattatct tttctatcgt
    14641 ttccagaagc agtccggaga gatccaccct ggccatttct agcggtacgg cgctttcgat
    14701 ccttgagagc aggagaaaac cctcggacat cctgatcaat ttgtcgagct ctttcatggt
    14761 gtccttgagc gaaagtctca tctccccggg cgaggagttt ttctcaagcg agagctggag
    14821 gttcgttttc atgacggcca ggggtgtccg cagttcatgg gctattttcg aggacatctg
    14881 gcgcgtgtct tcgacgagtc tttcgattct gtcgagcgcc ctgttgatct cgagctggag
    14941 tttctctatc tcccggccgg tgttttcaac ctctactctc gcgccgccga tctcggaacg
    15001 gctgatcctt tcgagctggt ttcccgcctg ttcaagcggc ttcatgagct ttctagtgaa
    15061 ggccatcccc accaggctca caaggagcag ggtgaaggct gccgagagaa ttataatcag
    15121 cagcaggtta ttcagatgtg cccgggttcc ttccagtctg tagaaggtca ctactttccg
    15181 gtccccgcct tcgatactct cgagagtcgc ccgatagcgg taatcacccg ccgttcccat
    15241 tcccggaccg acagaagaca aagtgcccgg ctcgatccgg aagcttgcca gcggtttttc
    15301 accagcgtac agagcgaagc cgaggttttc gtcgccggcc aggctcagga gcctcttctc
    15361 accctctccg gcgtacttat aggtgttgac tatgtcgttg tggaaggata gcagcaggcc
    15421 gtctatcctc ctgtattcga gcgttctgaa tatcatgaag agcgttatgc tgagcgcgaa
    15481 gacgaccgag ccgtacgtgg caaggtagag gagtgtgatt ttgtttttga tcgtcataga
    15541 cctcacggct tctccctcaa cctgtaaccc ctgccctgaa cggtctctat tatgtggtct
    15601 agcttcagcg cttcgagctt tttgcgtatg tagcggatgt aaacatccac tatgttgctg
    15661 cgcggttcgt agccgctctc ccagattttg tcggttatct cctctctggt gaagaccgtg
    15721 ccggggtttc tcatgagcag ctcgaggatc gagaattctc ttgcgctcag ctcgatcggt
    15781 ttctcgcggt agagaacggt ctgttttctg gagtcgagaa cgagctcacc gcaggcgatc
    15841 ttttcaccgc ccctgagctg tgcccttctc aggagagcct tgaccctggc cagcagctca
    15901 tgaagatcga agggcttggg cagatagtcg tcggcgccgg cgttgagccc ctctatcctg
    15961 ttgtcgagct cgcccagggc cgttagcata agtatcggca cggtggattt tatctcttcc
    16021 ctgagaaatc tgcatagcga gaggccgtcc atacccggga gcagaatgtc caggattatg
    16081 caatcgtagc ttcgctctag cgcgagatcc agcccttctt ccccgtcgaa ggccgcatcg
    16141 acggagtaac cctcgccttt gaggaaatcg gatatattgt cggagagttt tctctcgtcc
    16201 tcgataataa gcaacttcat ggcgtctatt tcttcccgtt tcccttgccg ggagtgtcgt
    16261 cggacttgcc tggatcgtcc ggtcggccgg gttcatccgg tttgccgggc tggtctgact
    16321 gaccgggctc ttcgggctgg cccggttttt cggccttcgc tttctctttc ttgatcttct
    16381 cggatacagc gttcagagcg ctccgtgtat ccgagacgct gacggttgag gcgaatgcgg
    16441 cgccgagagt gtaggcactg gattcggtta tcacgtgcca gagtggaccg ctctcggccg
    16501 tttccagata aagtcccaga atcccctctc tggtctggag agaaaccagc gaaaaggcgt
    16561 ctatgagcga gagcttcacg ttcactcccg ccagcggcgt ctcaaaccgg tcgtccgcgg
    16621 ttatctccag atcgggtgtc agcgtgagag agtttctgtc ggttatcacg gtgagcgtcc
    16681 cttcagaagg gagataatac agggcgcgaa cgttccctcc ggcctttttc acggccaggt
    16741 cgatcagttc aagatcgaga aaacgagatg gctctccgcc ctcgagcaac tggcttagca
    16801 actcctgctc ggcctccagc agggtcgtct gtccggggac ggagatcgaa aggaaaatca
    16861 gcaccgaaac ggccagtgcc ctgataactt tcctcattca agaactattg taccaagaga
    16921 gagatcccga tcgacaacga ccacgggttc gggataagtg taaagcggca cactggtgtc
    16981 gacgtcgaaa ccctcgtcgg tgtaattatc gaaaaacttg agcgtatagc tgccgggagc
    17041 gaccttgcag aagcggaact ctcccttttc ccacttcggc gtatttgtat gtgtgaaggt
    17101 gaggagcgta tcaccggttc cgtcgggcac gagcgcgacc agaagcggcg aggcctcacc
    17161 ggtcggagtt gcgacaccgg tcacatcgta aagacttcca tggcggatat gtatcaccgg
    17221 cgtcatgtgc actttatcct cgtggccctc ctttccgccc ttcgtctctt cccacttgcc
    17281 gttgatcttg agggatctgg agagatcgaa atccagcacc agaacggtgt cctcgtcctt
    17341 gctcagatct atgtcaaggt tgttgatctt tagcgagccg gaattgaccg tgacgggaaa
    17401 gactttgccg ttggcggtca gagtggcctc gctaatttcc atcctcagct ggttgtatat
    17461 gccgcttgcc tcgaactcgc ccaggtcgat ctcctcgcca atcagggtca tgatatccac
    17521 cgttatggct tcatcgagaa cgatcagcgg cgtctgctcg gtgggcgtcg cgccgtcggc
    17581 tccgtacagc gccatgctct cgatggtgac ctccagcttt tcaatatcct ccaggggaat
    17641 aacggcgtcg gtcagcgaca ctctgagcgt gccgttttca tcgggtttcg gaacgggaaa
    17701 gttgaaacaa ccgctgatta gaaagatagt cagaactacc agaaggaaaa agttggtttt
    17761 cttcatcgca aacacctcca gttacgcaat tttcggttca tggatcgagt atagcgggtc
    17821 ggcaattaaa gggaaattaa agaggccaaa gccttaacac tgaaagcgat acaaacgatg
    17881 agtatgatgc tatatttgat atgatcggat aaactgtcca gttcaggatg tcgggttatt
    17941 catggacaga tcgggcgagg ctgaaagcag atgaaaatag gtattcctaa cgctttgctg
    18001 tattattatt acggtccatt ctggacggag ttcttcaaag aactcggtgc ggaagtggtg
    18061 gtttcgcaca gcacggacaa aaaaaccgtc gatatggggg taggcatatc ggttccggag
    18121 atctgcgtcc ccataaagat attcaacggc cacgtcctcc agctactcaa gggtggggtc
    18181 gattacgttt tcataccccg catggcctcg gtggagaagg gtaagtactt ctgccccaaa
    18241 ttcatgggac tgccagacat ggcaaggcac agcatcccgg gagtcgccgg aaagcttctc
    18301 accatggacg ttcaatcgag gtcggagaat atattctccc cgaagatgta ctactcaatg
    18361 gccgaaaagc tgtccgtatc ggataagcag atcgagctgg cctgcaggaa gggcgccgag
    18421 ctctggcgaa agt
//
LOCUS       DAOOLY010000013        17939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3248_length_17939_cov_4.886491, whole genome shotgun sequence.
ACCESSION   DAOOLY010000013 DAOOLY010000000
VERSION     DAOOLY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17939
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_17939_cov_4.886491"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..535)
                     /locus_tag="PLD15_01385"
     CDS             complement(107..535)
                     /locus_tag="PLD15_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62579.1"
                     /translation="MKKPLVALSFFGAFLILFLSGCFLVDPSIKVVLLIENFKKYWEQ
                     EDPAALAGLYTDPAHINTATKTFAQIENTYDGMFSGRGVVSFTFISEKDIDFNATLTE
                     ADVQFTAVIDYTSGPNETKTYVWKVKRAGTRWLIYRSNNY"
     gene            702..1853
                     /locus_tag="PLD15_01390"
     CDS             702..1853
                     /locus_tag="PLD15_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62580.1"
                     /translation="MPRIIFFEALIYASLATKNMLSQYFDLAGYSSVQIGFLMTVLPI
                     VSLASSPFWFKLGSKIGERRIYYIVSIATAFLIWPVFLAGDFATNLLFMFVFSFFFSG
                     IVPIGDSLIMSRIKLTGGRFDRIRLCGTIGFAITSFCLSMVLGTSFIWLFVATSLLVS
                     ISAMTIGLKDHGTDALRGPSKKKTTDGTLFEYSIMTAGMFLGITLNSFHNSFIPLLTR
                     ERGMDVSAVGVIFAITAVSEIPFLLYADRLISRIGNLAVLEIGMGVIGVRMILISFTT
                     SVVSLYLAETLHGLTYILMYYSLFDYIHFRLPKEHLASAQSVFWVVRSGLTFIAGSIG
                     GGFLIERLSVTLAFRSFGVAGMAAAGIVFFIGLLKRTNSREKYKDTAGG"
     gene            complement(1913..3226)
                     /locus_tag="PLD15_01395"
     CDS             complement(1913..3226)
                     /locus_tag="PLD15_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62581.1"
                     /translation="MKDLGRNFWLYAIGRLVSLIGSGIQSLAIPLYILDLTGSGTIMG
                     TFMVITMLPRILFGPVAGVLGDRFNRKMIMVYMDFARGAAILAMAVLAGANSLTITVL
                     FIFQLLISTFDISFDPATAAMLPDIIESDKLLRGNSILGAINSLSYIIGPALGGILYG
                     MFGIEAVLILNGASYIASAISEIFIRYQQTTEKGKISLKSVFKDVVEGVRYMRKINGL
                     ILVMVFAMLSNFLLSPFFSVVFPFFARTIVGFTSEQYGFLQSGWVVGVLIGNVILGTL
                     LSKKRQGNLFAMGLTAETLILFLLTVFFFPYFIDLFGWASWRYFAALGLPILVTGIFN
                     AFVNTPLNTLFQKIVPTNYRSRIFSVISILAQIATPLGAAIYGFAVDRVPVHYLILVS
                     SIGNALLTLVFLLKGMTKLFDGKTPDNKTGLVEKSVEAIKEGASL"
     gene            complement(3533..4591)
                     /locus_tag="PLD15_01400"
     CDS             complement(3533..4591)
                     /locus_tag="PLD15_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB62582.1"
                     /translation="MYHRPDFSIMLYALGRAKEPGRVPFFELFADREIIEEVMGFKLA
                     DPGSESGKYFDQLASFYYELGYDYVPFYLIPRFPLADKIDSEDTALYRRSRRRWVNEK
                     DGPIRSLKDLEEARWPEPEESLDFELFRELGERLPPGMKIIGGAAGGPFEHASMLLGL
                     ERFSLAVYEEPELVDELVGKIGRILTGAARIISSFDCVGAYCFGDDLGYKTATIFSPK
                     HLRKLVFPWYSEIARIVHTAGKPFILHSCGNLATVMDDIIAAGIDAKHSFEDIITPVS
                     MAKKLWGERIAVLGGVDVDFLCKADPESVRKHTLKLLEECAPGGGYALGTGNTVANYI
                     PVKNYLAMLGAGNEFNSR"
     gene            complement(4624..5667)
                     /locus_tag="PLD15_01405"
     CDS             complement(4624..5667)
                     /locus_tag="PLD15_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPB62583.1"
                     /translation="MKRLVLLFVLLLAVSLFAVSTVLEGVASLLFGRYEGNETHFFLR
                     EIDSSLQLLLKSSDGELIFNRSRYLPVECAELCEDNYLCEPNPFKFTIITFDRDESGM
                     GVSCIAGGEVFRRKFYGPEGGDVFKITPVEPIDKLLPTAMEATPPVEAGTFLPHDLVE
                     VTLLDPSIKLDIRYARYDNFMGVPLYSQERAFLQQPAAHALVKASELLRSMGYGLVVH
                     DAYRPWYVTKMFWDATPDDLKEFVADPQKGSRHNRGCAVDVSLYILNTGEIIDFGGGY
                     DEFSERSYIDYPGGTSLQRSHRELLRETMVSVGFEPLQEEWWHFDYRDWKSYTIGNLT
                     FEELDSLQESSGF"
     gene            complement(5741..6751)
                     /locus_tag="PLD15_01410"
     CDS             complement(5741..6751)
                     /locus_tag="PLD15_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HPB62584.1"
                     /translation="MKKAILFLFIMLAALGFAWSGHDALTYTIVSRFSLPLEEYVQIT
                     PYTYADVDTHPYNPAITGFYDYLGKNYMPEEDIDLFIPVFPNPEPVNNTAPVWQILSV
                     YAYEPDMGMDKGLPMEGIEKLLGDSQGIRHMEYRLLCFIRVGEVTDMVQYFSDLSELA
                     YGRGDHYWAYRFMARAMHYLEDVGQPFHTFPAPLFELLKLPFNMDKWQTVFAKYHFAY
                     DFYGGYLLWGQYGPLVKAIDEVPAKTIKSPKQAAVDLRGFSRGKLNPVYYELKHLMKD
                     ELETEEIVWLGKSYFDELVKAGKTEKLDKMTVEILRETASYVKGYINYMFDKFEAIDS
                     NM"
     gene            6955..8208
                     /locus_tag="PLD15_01415"
     CDS             6955..8208
                     /locus_tag="PLD15_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB62585.1"
                     /translation="MSSNEGREIAGVFRGSFAIRGFEDIEFNFQLLRSLGAVSYGGGT
                     VGEILSIVPAIRPEEPETWVTQFKDLAIRAKKLGLDLMQKGRMKSAHEAFLRASSYFR
                     AAEYYGDPRDSKNRYIGMESRDCFIYAFRTANLEFEAVRIPYGDDFIPAYWISPLTQS
                     SQTKKRTIMMMSGFDGTSEEMYFQSGAAALNRGYSLLLFDGPGQVGMRRFSTETPFRA
                     DFEVAVAKVVDYLLSRGDVDPSKIALYGISLGGYFSLRAAAHEPRIKALISNSPITDI
                     YKYMIGVAGEGAMNSSQDIGLDYIDEIPAEYLSKGKKLQILNIILRYGERSMFAAFER
                     MKEFTVGEAIKNIRAPFLGMVSQGEGLGAMRQAEEAVKGVSGPSSLRVFTKDEGADSH
                     CQLTNLPLSNAVLLDWLDEVFGVRS"
     gene            8205..8900
                     /locus_tag="PLD15_01420"
     CDS             8205..8900
                     /locus_tag="PLD15_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPB62586.1"
                     /translation="MKRIGLLGGMSWESSLEYYRLLNEAVKERLGSLNSADCVMVSID
                     FQPVADMMKLNNWKGIARVLEDVSRDLKRAGADFLVICTNTMHLLVREIEDASGLKVL
                     QIGEAVAERITAAGLKTVGLLGTRFTMERSYYRNTLGRHGIEVIVPQERDRESVDRII
                     FQELCRGIFTEESRESYRSIISKLQSEGASGVVLGCTEIPLLIKQSDVEIPVFDTTAI
                     HAAAAVEFALADN"
     gene            8984..10531
                     /locus_tag="PLD15_01425"
     CDS             8984..10531
                     /locus_tag="PLD15_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPB62587.1"
                     /translation="MRRTLLLTLVILLVSAMGLASVYSNASSNNLEGVKEDLKSGGNI
                     NELGYNNKSALMTAVDYGYAEIARFLIESGADIFLIDNDGFTALHYAARKGLTEIAKL
                     LVERGAEIDKYPDSSKFYAGYTPLILACDSSRDDKTLEVVKYLVEKGANLEKVEFAFK
                     SPLTAAVFAKSTNTIRFLLESGANPNNALNDGRTPLYLAISEGLPFEAVDVLLAKGAD
                     VSLGTEYYTPLGLAVSKSNHDVTKRLIDFGADYLKVDSLGNTILHNAARSGSTKNIEL
                     FVGLGIDINSINMEGDTPLHIAAEREYFRNVELLISLGADVLAKNNNGYLPLHIHLAV
                     YKESKDTIELLLSKGVSINQNEVEFGFSPLHLAAGNGMLEMVKFLVEKGADISIQADK
                     KIAPLHMAVGRYGKVEVIDYLLEKGANINVEDEDGKIPIFYAVIEKNLEAASHLAERG
                     ADINHVDLLGMTTAHYAVQDKWDGIEMLKILRQFGVPVNIKDNSGKMPEDYAASEEIK
                     EFLRSLY"
     gene            complement(10789..11418)
                     /locus_tag="PLD15_01430"
     CDS             complement(10789..11418)
                     /locus_tag="PLD15_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPB62588.1"
                     /translation="MKKMEESMKEIIQSEKIMAVIRSDSVDRCVEIARSCYRGGIKIL
                     EITFTVPDAEKAISIISRELPEAYVGAGTVLTDGQFDLAIRSGARYVVSPHLSEELAI
                     HSRAMEVLYLPGIMTPTEYVKATGLGLSMVKVFPGDVLTPAFVKSLRGPFPEARCVVS
                     GGVSLDNINLWFEAGTAAVGIGSDLTKGTPLQIEEKARQYSQKIKDLQS"
     gene            complement(11415..12338)
                     /locus_tag="PLD15_01435"
     CDS             complement(11415..12338)
                     /locus_tag="PLD15_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62589.1"
                     /translation="MLVLRKEDLWKIKYFMQDYGRDLEEKLFSYLYEDDSGEKVIEEL
                     SRYQNEDGGFGNALEPDFRLPYSSPMATSVAFQIITKLGYDSTAKREMISRAVEYLEK
                     TFIPGRMGWLSVPESVNDFPHAPWWHFDSEKGMSSIDINWGNPTAEIIGYLHRYREYT
                     SLDVDALIEKAIEYLNGMKEFGSFHEIFCFERLHHQLDMERAGRLEDKIVQAVNSLTC
                     CDEDEWKSEYTARPLDFATGPDRTFGIDGALIEKNLDCYVQTLQSNGRIKPTWSKSIY
                     DGDLAPAWDEWVAILTLRVLETLKNFGRIVL"
     gene            complement(12371..13999)
                     /locus_tag="PLD15_01440"
     CDS             complement(12371..13999)
                     /locus_tag="PLD15_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62590.1"
                     /translation="MHKFLTVILLVLLAATLLARDLTVRSSSSRFDFEEISGGSISLP
                     IDTTGHFLPGLELVRGDIWVTAATQDPLFRKLSGDRIEPSLVERWEWRDGNKELHIKL
                     RDEIHWFDGTPITTNDLEYSLAVWKVSEDSQLYGILNDLSYVEMKTLDDKSAVFYFNR
                     PFPEFTGYLRTGLLARHIYEGKLGLKPEEVAVSQYSGKPPLEATSGPFYLAEGSEKGK
                     IILKRNPQWHGGKGSEHYPLYFAEWPEHALLDEIRLIEEADQEERIEMFLKGELSMLF
                     NEEGATTELINDAKNGNYRYQFTPDGSYYALILNHRNDFLSKSSLRKALRMALDVEKL
                     SGTIGIGSTAALLPIDVRTAFLQELLSQIGEFPYDPGRAKELIVSAGYGKGYTLTIKV
                     HQGVDKKFTDMLVKMWKEIGVDLKVERLDWGSLLGDVYSADYELAFFRVEAFDYPEIT
                     PWTVENADDLKTGWEVGYVNPQLFRIERKAATEGLWKDRIPYYLGAMKLWLEDMPIVV
                     VSYSQKITFWRQELNGPVPGSGELFDNLAEWFVH"
     gene            14676..14804
                     /locus_tag="PLD15_01445"
     CDS             14676..14804
                     /locus_tag="PLD15_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62591.1"
                     /translation="MKIALSGEIRKDRISCSVVLPPFIDKPDSLMEFPQDSRQEVA"
     gene            14921..15715
                     /locus_tag="PLD15_01450"
     CDS             14921..15715
                     /locus_tag="PLD15_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62592.1"
                     /translation="MADALFANNVVKFFKKSKKEIKALKGVSLRIKEGEIYGLLGPNG
                     SGKSTFIRIASTLLIPDRGSVSVFGHDVEREPGIVQKLINRVSVEASFFRKLSAIENL
                     AFSAGLYGISKKEAIKKIYYLSERFGLDEKRLKDPLEDFSRGMQQKVAIVRAFMTEPR
                     MLLLDEPTTGLDPRAKKDVQSLIRDVKSNMNTTMLLTTHDMQEAEKLCDYVGIIHLGK
                     IVAEGKPQELKNQLRGKVDNPTFEDVFMEYTGMSLEDAQYREVETA"
     gene            15712..16542
                     /locus_tag="PLD15_01455"
     CDS             15712..16542
                     /locus_tag="PLD15_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62593.1"
                     /translation="MSSQALVRELRASWAFIERNFNFTKRYWKWELVFFVYTIANSVT
                     MGFIGKGIEAFSGTKLDTSYLILYMLLGSILWGYLSILFEIVAETVAWERWEETIEYT
                     FMAPVRRATHLLSTCGYAILYGILRSGLILLAVSIFFDLNLEKANLLSAVGILAVSSF
                     SFVGLGIVAAVLPLISPEKGAQVVHIFQALLLMFSGVYYEVDVLPQWMQQVGRLSPAT
                     YALRGMRSSILEGKGMIEMWGDIWPLILLGGLLLPSGLLFFRKMEGWAKRKGVLKRSG
                     "
     gene            complement(16567..>17939)
                     /locus_tag="PLD15_01460"
     CDS             complement(16567..>17939)
                     /locus_tag="PLD15_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB62594.1"
                     /translation="LPPEFYKNIGRNEVDIERWLKNLGKYHRLQHEHMVFIESTPDMV
                     FLKDKEFKYLIANRALSKYFKKSIDEIIGKTDFELMQYEVASKCRESDMKAVEHNRLI
                     VTKERTANKVYETYKFPVKLANSDTGIGGYIRDITLSQEYLDNIKKYGVIFHNANDMI
                     FIVRACDLRVLDVNRAVIKTYGYSREEALKLSIPDFTAPGSVSTIDEQLLSAYEDGAK
                     YETLHRKKDGSIFPVEVSARFIMLRGEEVILDIVRENTERKNNEEIIRKSMLTLLNTL
                     LRMVEIRDPYTAGHQRRVSQLACAIAERLNLGESRVQLIRIASMLHDIGKISIPGEIL
                     SKPGKLSKMEFDLVKLHPVTGYDLLKEMSFDLPIATIVKQHHERLDGSGYPDGLKGDE
                     ITLEARIIAVADVVEAMSSHRPYLSSLGLKKALNEISAGVDILFDRKVVQACIQLFEE
                     KFTFSQ"
BASE COUNT         4484 a         4384 c         4474 g         4597 t
ORIGIN      
        1 gcgtttgtat cgtagataat cttcgatcat tctggccggg tctctgtaga tgagattcaa
       61 cacgctcacc ccggtaagca gagttctcat cgcgatcgga gtcccttcaa taattgttgg
      121 aacgatagat tagccacctc gtacccgctc ttttaacttt ccacacgtat gtttttgtct
      181 cgttcggacc gcttgtgtaa tctataacgg ctgtgaactg gacgtccgct tcggtaaggg
      241 ttgcgttgaa atctatgtcc ttctcgctga tgaacgtgaa agacaccaca ccccttcccg
      301 agaacattcc gtcgtaggtg ttttctatct gggcaaaggt tttcgtagcc gtatttatgt
      361 gggccggatc cgtatagagg cccgcaagcg cggctggatc ttcctgttcc cagtactttt
      421 tgaagttttc gatcaacagg accactttta tagaaggatc gaccaagaag cacccgctca
      481 agaatagtat caggaaagcc ccaaaaaacg ataaagcaac taacggtttt ttcatgatga
      541 cacctccctg ccgatattga aatctatatc ggtatcgtca accgctcagt cccccgcctg
      601 ctacgatgac attttactcc tttgcctgtt agaaccttcc cttcatataa actcccgctg
      661 gtggtaatat aattgcctta tcgacggtga aggacagtat catgcccagg ataattttct
      721 ttgaagcgtt gatatacgcc agcctggcaa ccaagaacat gttgagccag tacttcgatc
      781 tggcaggtta ctcttcggtc cagatcggtt ttcttatgac cgtattgccc atcgtgtcgc
      841 tcgcatccag tcccttctgg ttcaaactgg gttcgaagat aggcgagaga cgtatttatt
      901 acatcgtttc gatcgccacc gccttcctca tctggccggt cttcctggcc ggagatttcg
      961 cgaccaacct tttgttcatg tttgtttttt cctttttctt ttcggggatc gttcctatcg
     1021 gggattcttt gatcatgtct agaatcaaac tgacgggcgg tcgattcgac aggataaggt
     1081 tgtgcggaac gatcggtttc gccatcactt ccttctgcct gagcatggtt ttgggcactt
     1141 ctttcatatg gcttttcgtg gcaacttctc tgttagtctc tatctcggcg atgacaatcg
     1201 gcctgaaaga ccacgggact gatgctttac gcggaccgtc gaagaaaaaa acgaccgacg
     1261 gaacgctttt tgagtactcg ataatgaccg ccggcatgtt cctgggcata actctcaact
     1321 cgttccacaa ctccttcata cctcttctca ccagagagag gggtatggat gtctctgccg
     1381 taggcgttat ctttgcgata accgcggtat cggaaatacc ctttcttctt tatgccgaca
     1441 ggctgatatc cagaatcgga aatctggccg ttctggagat cggtatgggt gtgataggcg
     1501 tcagaatgat actaatatcc ttcaccacga gcgtcgtatc cctgtatctc gccgagacgc
     1561 ttcacggact cacttatatt ctcatgtact attcgctttt cgattacatt cacttcaggt
     1621 tgccgaagga gcatctggct tctgcccaga gcgttttctg ggtcgtaaga tccggtttga
     1681 cctttatcgc cggctcgatc ggagggggtt tcctgatcga aaggctttcg gtgactctgg
     1741 ccttcagatc cttcggggta gccggaatgg cggcggccgg tatcgtattc ttcatcggtc
     1801 tgttgaagag aactaattca agagagaagt acaaagacac cgccggaggc tgaaatactc
     1861 cggcttttga tggtgtgaaa cgttgcttaa cttctcgcga aaggagttgg cgctaaaggc
     1921 tggctccctc ttttatcgct tctactgatt tctcgacaag acccgtcttg ttgtcggggg
     1981 tcttcccatc gaagagcttc gtcatgccct tcaagaggaa taccagcgta agcaaggcgt
     2041 tgcctatgga cgataccagt atcaaatagt gtaccggcac gcggtcgacg gcaaagccgt
     2101 agatcgccgc acccagagga gtcgctatct gagcgaggat ggatattacc gaaaagattc
     2161 gcgacctgta attggtggga acgatttttt ggaacaacgt gttcaaaggc gtgttgacga
     2221 aagcgttgaa aatgcccgtg acgagtatag gaaggccaag agcggcgaaa taccgccagc
     2281 tggcccagcc gaacagatcg atgaaataag ggaagaagaa cacggtcaga agaaagagta
     2341 tcagggtttc ggccgtaaga cccatcgcga agaggttgcc ctgtctcttc ttagatagaa
     2401 gcgttccgag tatcacgttt cctatgagca ctcccacgac ccagcccgat tgaagaaagc
     2461 cgtactgttc actggtgaaa cccacgattg ttctggcgaa aaacgggaag accaccgaaa
     2521 agaagggaga tagaaggaag ttcgagagca tcgcgaaaac cataacgaga atgaggccgt
     2581 tgatctttcg catatagcgc acgccttcga ctacatcttt gaatacgctc ttcaaggata
     2641 ttttgccctt ctctgtagtt tgctgatacc ttatgaagat ctcgctgatc gcggatgcga
     2701 tgtacgaggc accattgagg atgagaaccg cttctattcc aaacattccg taaagtatcc
     2761 cacccagtgc cgggccgatt atgtatgaga gggagtttat cgctccaagt atcgagttac
     2821 ccctgagcag cttgtcgctc tctatgatgt cgggcagcat cgccgcggtt gcgggatcga
     2881 aggagatatc gaaagtggat atcaaaagct ggaagatgaa aagaaccgtt atggtcagac
     2941 tgttggcgcc tgccagtacg gccatcgcaa gaatcgccgc ccctcgcgcg aagtccatgt
     3001 ataccatgat catttttcga ttgaaccggt cgcccagaac gccagcaaca gggccgaaga
     3061 gaatccttgg aagcatggtt atcaccatga aagttcccat gatcgttccc gaaccggtaa
     3121 gatcgagtat ataaagcgga atcgccagag attgaatacc gcttcctatc aaggatacca
     3181 atctaccaat cgcgtaaagc cagaaatttc tcccgagatc tttcatacga tcacctcaga
     3241 tatatttctt atatggtccg ataatatcat aaaatatata aataaatcaa gtcctatata
     3301 aatatttttt atattacgga taatttttat agttttcagt acttttccag aatttactaa
     3361 tagcaccata gttctcattg tcggccatgc tagacagtat tttctgaaaa accttcagtt
     3421 agtatgcttc ggaaaaaaca tagataaata gctagaagtg caaaatactc aaatataaat
     3481 tgaaaacttt cagggaagta aaaatcgatt caacggcgaa gcggtttcca gttcagcggc
     3541 tattgaattc gttaccggcc ccaagcatag ccaggtagtt tttcaccggt atgtaattgg
     3601 cgaccgtgtt acccgtaccc agagcgtagc ctccacccgg cgcacactct tccagcagtt
     3661 tcaaagtgtg ttttctcaca ctttcggggt cggccttgca gagaaaatcg acatcgacgc
     3721 ccccgaggac ggcgattcgc tcgccccata gtttttttgc catagagacc ggcgtgatga
     3781 tatcctcgaa tgaatgcttg gcatctattc cggcggctat tatgtcgtcc ataacggtgg
     3841 ccagattgcc gcagctgtga agtatgaaag gttttcccgc cgtgtgaacg atccgggcga
     3901 tttcactgta ccaggggaag accagttttc tgagatgttt gggcgagaag atcgtggccg
     3961 tcttgtagcc aagatcgtcg ccgaagcagt acgcccctac gcagtcaaaa gaggagatga
     4021 ttctggccgc acctgtcagt atcctaccga ttttcccgac cagctcatcg accagctccg
     4081 gctcttcgta aaccgccagc gagaacctct cgagaccgag aagcatgctc gcgtgctcga
     4141 agggtcctcc cgcggctccg cctataattt tcatgcccgg aggaagacgt tccccaagtt
     4201 ccctgaagag ttcgaaatcc aaagactctt ccggttccgg ccagcgagct tcttcgaggt
     4261 ctttgagaga ccggatcgga ccgtcctttt cgttcaccca tcttcttctg gagcgcctgt
     4321 aaagggcggt gtcctcgcta tctatcttgt cggcgagcgg gaagcgggga atgagataga
     4381 aaggcacata atcgtacccc agctcataat agaaactcgc cagctgatcg aagtacttcc
     4441 cgctttcact accgggatcg gccagcttga aacccatcac ctcttcgatt atttccctgt
     4501 cggcgaagag ctcgaaaaaa ggtacgcgcc cgggctcttt tgcgcgcccg agtgcgtaca
     4561 gcatgatcga aaaatccggt cggtgataca agacgatgcc tcccaggcta gaagccaatc
     4621 tatctaaaag cccgaactct cctgaagaga gtccagctct tcaaaggtaa ggtttccgat
     4681 agtatagctc ttccagtcgc gataatcgaa gtgccaccac tcttcctgca aaggttcgaa
     4741 gccaacggag accatggttt cccttaggag ctccctgtga gatctctgca ggctcgtgcc
     4801 gccggggtaa tctatgtacg acctttcaga gaattcgtcg taaccaccgc cgaaatcgat
     4861 aatctctccc gtattcagta tgtatagaga gacatcgact gcacagcccc tgttgtgtct
     4921 ggaacctttc tgcggatcgg ctacgaactc tttgaggtcg tcaggggtcg cgtcccagaa
     4981 catctttgtg acataccagg gtctgtaggc atcgtggacc accagcccgt aacccatcga
     5041 tctcaggagc tcgctcgctt tcacaagggc gtgtgcggcc ggttgctgga gaaaggctcg
     5101 ctcttgtgag tataggggca ctcccatgaa gttgtcgtat ctggcataac gtatgtccaa
     5161 ttttatcgag ggatcgagca gcgtcacttc cacgaggtcg tgcggcagaa aagtgccggc
     5221 ttcgaccggt ggagtcgctt ccatagctgt cggaagcagt ttgtctattg gttcgacagg
     5281 tgttatcttg aaaacatctc ccccctccgg tccatagaat tttctcctga aaacctctcc
     5341 tccagcgatg cagcttactc ccatgccgga ttcgtctctg tcgaaagtta tgatcgtgaa
     5401 tttgaaggga ttgggttcac atagatagtt atcctcacat agctccgcac attcgaccgg
     5461 cagatatctc gacctgttga agatgagttc cccgtcgctg cttttcaaaa gcaactggag
     5521 cgaggagtca atctctctca aaaagaagtg tgtctcgttc ccttcgtacc ttccaaagag
     5581 caaagaggcg actccctcta agactgtcga aacggcgaac agggaaactg ccagcaagag
     5641 tacaaacaaa agaaccagtc gtttcatctt tccacctcca ggccaaataa atctatctat
     5701 acgggatagg acttatcgga gggattgtcc aaagcgaacc tcacatattc gaatctatcg
     5761 cttcgaactt atcgaacata tagttaatgt agcccttcac ataggatgca gtttctctga
     5821 gaatctccac agtcatttta tccagttttt ccgtctttcc tgccttaacg agttcgtcga
     5881 aatagctctt tcctagccag actatttctt ccgtctcgag ttcgtccttc atgagatgct
     5941 tcagttcata atagacggga ttcaactttc ctctcgagaa gcctctcaga tcgacggctg
     6001 cctgtttcgg actctttatg gtcttcgccg ggacttcgtc tatggccttg accagtggcc
     6061 catactgtcc ccagagcagg tagccgccgt agaagtcgta agcgaagtga tacttggcaa
     6121 agacggtctg ccatttgtcc atgttgaacg gcagcttcag cagttcgaat aaaggcgcgg
     6181 ggaaagtatg aaagggctgc ccgacatctt caaggtaatg catcgccctg gccataaacc
     6241 tgtaagccca gtagtgatct cccctgccgt aggccagttc ggaaaggtcg ctgaaatact
     6301 gtaccatgtc ggtaacctct ccaaccctaa taaagcacag caacctgtat tccatatgac
     6361 ggatgccctg actgtctccc aggagtttct caatcccctc cataggcagt cctttgtcca
     6421 tacccatgtc cggttcataa gcataaacgg aaagaatctg ccacacaggg gcggtattgt
     6481 tcaccggctc gggattggga aagaccggaa tgaagagatc gatatcttcc tccggcatgt
     6541 agtttttacc cagataatcg tagaacccgg ttattgcggg attgtaggga tgggtatcca
     6601 catcggcgta tgtatagggc gttatttgca catattcttc taaaggcaag gaaaacctcg
     6661 agactatcgt ataggtcagt gcatcgtgcc cggaccaggc aaatcccaga gcggctaaca
     6721 tgataaataa aaataagatc gcctttttca taatcaacac ctccggattc ataatagcag
     6781 aaggatagag tttttttgct taaaactccg ggttcactgc aaatagttgg ttttagcgga
     6841 gagtgatgtg cacttttgtg agtctatcaa attgaagccc cgtttgtttt tgtacgatac
     6901 aatggttgta tctgctagta tttcagatag aatctttcaa gggaggtgat cggaatgtct
     6961 tcgaatgaag ggagagaaat tgccggggtt tttcgaggtt catttgccat ccggggattc
     7021 gaagatatag agttcaactt tcaactcttg aggtcgcttg gagcggtttc ctacggtgga
     7081 ggaacggtcg gcgagatact atcgatcgtg ccggctataa gacctgaaga gcccgaaacc
     7141 tgggtgacac agttcaagga tctggccatc agggcaaaga aactggggct ggatctcatg
     7201 caaaagggca gaatgaagag cgcccacgaa gcttttttga gggcttcaag ctatttcagg
     7261 gccgccgaat attacggaga cccgagggac tcaaaaaaca gatatatagg tatggagagc
     7321 agagattgtt tcatatacgc cttcagaacg gcaaatctcg agtttgaagc cgtcaggata
     7381 ccttacggag acgatttcat tcccgcgtac tggatctcgc cgcttacaca aagctctcag
     7441 acaaaaaaga gaaccatcat gatgatgagc ggcttcgatg gaacctccga agagatgtat
     7501 ttccagagcg gggccgcggc actgaacagg ggttactcgt tgttgctttt tgacgggccg
     7561 ggtcaggtgg gtatgaggag attctctacc gaaacgcctt tcagagcgga tttcgaagtg
     7621 gccgtggcga aagtggtaga ttatctgctc tcgcgtggcg acgttgatcc ctcgaagatc
     7681 gccctttacg gtataagcct cggtggatat ttttcgctgc gcgcggcggc tcatgaacct
     7741 cgaatcaagg ccttgatctc aaattcaccc ataacggaca tctacaaata catgatcggc
     7801 gtggccggcg aaggggccat gaactcatca caggacatag ggctcgacta tatcgacgaa
     7861 atcccagcgg agtatctgag caagggtaag aagctccaga ttctcaatat cattctgcgt
     7921 tacggagaga ggtcgatgtt cgcagccttc gagagaatga aggagttcac cgttggcgag
     7981 gcgataaaga acatacgcgc acccttcctt ggaatggtaa gccagggcga gggactcggc
     8041 gcaatgagac aggcagagga agccgtcaaa ggcgtttccg gaccttcgag cctgagagtc
     8101 ttcacaaagg acgagggggc ggactctcac tgccagctca ccaatctccc cctctccaac
     8161 gccgtccttc tcgactggct ggatgaagtc ttcggagtca ggtcgtgaag aggatcggac
     8221 tgctgggagg catgagctgg gaatcttcgt tggagtacta ccggttgctc aatgaagccg
     8281 tcaaagagag acttggcagt ctcaactcgg ccgattgcgt gatggtctct atcgacttcc
     8341 agccggtggc cgatatgatg aagctcaaca actggaaagg gatcgcccgt gttcttgagg
     8401 atgtatcgag agatttgaag agagccggcg ccgactttct ggtgatatgt accaatacga
     8461 tgcacctgct ggtcagggag attgaagatg cgtcgggatt gaaagtcctt caaatcgggg
     8521 aagccgttgc ggagagaata accgcggccg gcctcaagac cgtcggactc ctcgggacgc
     8581 gcttcaccat ggagaggagt tattaccgca acactctcgg cagacacggg atcgaagtga
     8641 tcgttcctca agagcgagac agggagagtg tggacaggat aatcttccag gagctctgca
     8701 ggggaatctt cactgaagag tccagagaga gctatagatc gataatctcg aaactccaga
     8761 gtgaaggagc cagcggagtg gtgctaggtt gtaccgagat accactactc ataaagcagt
     8821 ccgacgtcga gattcccgtt ttcgacacca cggcgattca cgcggctgca gcggtggagt
     8881 tcgcgcttgc ggacaattga atttgcaagt ctctcctgaa gagaagaagc agaatctagt
     8941 aacctataat gtagtactgt ctaatacaaa ggaggtaaaa cttatgagaa gaactctcct
     9001 gcttacgctg gtcatattgc ttgtatctgc tatgggcctg gccagtgttt acagcaacgc
     9061 atccagcaac aaccttgagg gagtaaagga agatctcaaa agtggtggca atataaacga
     9121 actgggctat aacaacaaat cggctctcat gacggccgtc gattacggct atgctgaaat
     9181 cgccagattt ctcatcgaat ccggtgcaga tatttttctg atagacaacg atggattcac
     9241 tgccctgcat tacgctgcca gaaaaggact gacggagatc gcgaaactac tcgtcgaaag
     9301 aggtgccgag atcgacaagt accccgacag ttcaaaattt tacgccggtt ataccccact
     9361 gatactcgcc tgcgacagtt cgagggacga taagactctt gaagtagtta agtacctcgt
     9421 agaaaaggga gcgaacctgg agaaggtcga gttcgctttc aaatccccgc tgacggcagc
     9481 tgtctttgca aaatccacaa atacgataag gtttttgctc gagagcggtg ccaatccgaa
     9541 caacgcgctc aacgacggta gaacgccact gtatcttgcc atatcggaag ggttaccctt
     9601 cgaggccgtg gatgttcttc tggcaaaagg cgcggatgta tcgctgggaa ctgagtacta
     9661 tactcccctg gggctggccg tttcgaagtc taaccatgat gtcaccaaaa gattgataga
     9721 cttcggcgcc gactatttga aagtcgattc tctgggtaac accatactgc acaacgctgc
     9781 acgcagcggc tcgactaaga atatagagct gtttgtcggg ttgggaatcg atatcaactc
     9841 cataaacatg gagggagata cgccccttca catcgcagcc gagcgtgaat acttccgcaa
     9901 tgtcgaactc ctgatatcgc tcggggccga tgttctggca aagaacaaca acggatatct
     9961 accgctgcat atacatctgg ctgtatataa agagagcaaa gatactatcg aattgctcct
    10021 ttcgaaggga gtgagtataa accagaacga ggtcgagttc ggattttcgc cgctacacct
    10081 cgccgcaggg aatggcatgc ttgagatggt taaattcctt gttgagaagg gcgccgacat
    10141 atccatccag gcggataaaa agatcgcgcc cctgcacatg gctgtgggaa gatacggtaa
    10201 agtcgaggtt atcgattact tgctggagaa gggggcgaat ataaatgtcg aggacgaaga
    10261 cggcaaaatt cccatcttct acgcagttat tgagaaaaac ctcgaggctg cttctcatct
    10321 ggcagagaga ggggccgata taaaccatgt ggatttactt ggaatgacga ccgcacatta
    10381 tgccgttcaa gacaagtggg acggtataga aatgttgaag atccttcgcc agttcggagt
    10441 acctgtgaat ataaaggata atagcggaaa gatgcccgaa gattacgccg cctctgaaga
    10501 gataaaggag ttcctgagat ctctctactg agtgtggcgg gttttacgtt cgaacgaaag
    10561 taattcaaaa gaactggaga gcaggaagag ccgtccttgg gaaacctgag gctagaggta
    10621 agaggcgaga ggctggaagg tcgataagat ctcgactttc ttacctctga cctctccccc
    10681 tcttacctct gtttagagac agacgagatt agatcccgta taagaacact acgggatgac
    10741 accaagggaa catttcaggg ctggcttctc acggccaaag cgagagagtc atgactgaag
    10801 atccttgatt ttttgagaat actgtctggc cttttcctct atctgtaagg gcgttccctt
    10861 cgtgaggtcg gagccgattc cgacagctgc agtgccggct tcgaaccata gattaatatt
    10921 gtccagactg acgccgcctg agaccacaca tcgggcctcc gggaagggac ctctgagaga
    10981 cttcacgaac gccggtgtga gaacgtctcc cgggaaaacc ttcaccatcg agagtccgag
    11041 gccggttgcc tttacgtact ccgtgggagt cattattcca ggaagataaa gaacctccat
    11101 cgccctggag tgtatcgcga gctcttcaga gagatgggga ctgaccacgt atctcgcgcc
    11161 gctgcggatg gcaaggtcga actgtccgtc cgtaaggacc gttccggccc caacgtaagc
    11221 ctccggaagc tcccgcgaga tgatcgatat agctttttcc gcatcgggta cagtgaaggt
    11281 tatttcgagt atttttatac ctccacgata gcaactcctg gcgatctcta cacagcggtc
    11341 cactgaatcg cttctgatca cggccattat tttttccgat tgtattatct ctttcatgct
    11401 ctcctccatt ttcttcatag aacaattctt ccaaagttct tcaaagtctc gagtaccctg
    11461 agagtgagga ttgcgaccca ttcgtcccac gcaggagcga ggtctccgtc gtaaatgctc
    11521 tttgaccatg tgggtttgat tctgccgttg ctctgaagag tctgtacgta gcagtcgagg
    11581 ttcttctcta taagtgcgcc atcgatgccg aaggttctgt ccggcccggt ggcaaaatcg
    11641 aggggccgcg ccgtgtactc gcttttccac tcatcctcgt cacagcaggt gagactgttc
    11701 accgcctgaa cgattttatc ctcgagcctg ccggccctct ccatatcgag ctgatgatgg
    11761 agtctctcga agcagaaaat ttcgtggaag gaaccgaatt ccttcattcc attcaggtac
    11821 tcgatggctt tctcgatcag cgcatccaca tccagagacg tgtactccct gtagcgatgc
    11881 aggtaaccta tgatttcagc cgttgggttg ccccagttta tatctatcga cgacatgccc
    11941 ttttcgctgt cgaaatgcca ccagggcgca tggggaaaat cgttaacgct ctccggcaca
    12001 gataaccagc ccattctgcc gggtatgaag gtcttctcca gatattctac ggccctggaa
    12061 atcatttccc gcttggcggt gctgtcgtaa ccgagttttg tgataatctg aaaggctacc
    12121 gatgttgcca tcggtgagga gtatggaagt ctgaagtccg gttcgagtgc gtttccaaag
    12181 ccaccgtctt cgttctggta tctggatagc tcctcgatca ccttctcgcc cgaatcatct
    12241 tcgtacagat aggagaacag cttttcttca agatctctgc cgtaatcctg catgaaatac
    12301 ttgatcttcc agagatcttc ttttctcaat acaagcaatt tttcgccctc ctttggtttc
    12361 cggaaatcgt tcagtgcacg aaccattcgg ccagattgtc gaacagttct ccacttcccg
    12421 gcaccggtcc gttcaactct tgcctccaga acgtgatctt ctgactatag gataccacta
    12481 cgatcggcat atcttcgagc cataacttca tagcccccag gtagtacggg atcctgtcct
    12541 tccagaggcc ctcggtggcc gcttttctct ctatcctgaa caattgtgga tttacatatc
    12601 ccacttccca gccggttttt aagtcgtcgg cattctccac cgtccaggga gtaatctcag
    12661 gataatcgaa tgcttcaact ctgaaaaagg ccagctcata atcggccgaa tatacgtcgc
    12721 cgagcagact tccccagtct agtctctcca ccttcagatc cactccgatc tccttccaca
    12781 tcttcacgag catatccgtg aacttcttat cgactccctg gtgaaccttg atcgtaagtg
    12841 tgtatccctt tccgtacccg gcactcacaa tgagttcttt tgcccttcca gggtcgtagg
    12901 gaaactcccc gatctgcgac aggagttcct gaagaaaagc tgttcggaca tctatgggca
    12961 gaagagctgc ggtagagccg atcccgatcg tgcccgacag tttctcgaca tccagagcca
    13021 ttctcaaggc ctttctcaaa gagctcttag agagaaagtc gttcctgtga tttagaatca
    13081 aagcataata agaaccgtcg ggagtgaatt ggtacctgta atttccattc tttgcgtcgt
    13141 ttatgagttc tgtcgtcgca ccttcttcgt tgaagagcat gctcagttcg cccttcagaa
    13201 acatctctat cctctcttcc tgatcggcct cttctatcag tcttatttcg tccagaagag
    13261 catgctcggg ccactcagcg aagtatagag ggtagtgttc cgaccctttt ccgccgtgcc
    13321 attggggatt tctcttaagt atgatctttc ccttctcgct cccctctgcc aggtaaaaag
    13381 gtccggaggt agcctccagg ggaggtttac ctgaatactg gctgacagcc acctcttccg
    13441 gtttcaatcc gagcttccct tcgtatatgt gtctggccag taatcccgtc cttagataac
    13501 cggtgaactc gggaaatggt cgattgaaat agaaaacggc gcttttgtca tccagggttt
    13561 tcatctctac gtaagagaga tcgttcagaa ttccgtagag ttgcgagtcc tcgctaactt
    13621 tccaaactgc cagcgagtat tctagatcat tcgtagtaat cggagttcca tcgaaccagt
    13681 ggatctcgtc tctgagtttt atatgcagtt ctttattccc gtctctccac tcccaccttt
    13741 ctacgagcga aggctcgatt ctatccccgc tcaacttcct gaagaggggg tcctgagtgg
    13801 cagccgttac ccagatatcg ccccggacca gctcaaggcc gggcaggaag tggccggtag
    13861 tatctattgg gagagagatg gatccaccgg atatttcttc gaagtcgaac cgggaactgc
    13921 tggatctgac cgtcaaatct ctcgcgagta acgtagcggc gagcagtacg agtaaaataa
    13981 ctgtcaaaaa tttatgcacg ggcaacctcc agaacattca tcagcacaat tatatcgaaa
    14041 ttacccgctc tgtggaaaag agtatttgag tatcgggata cgggaaagag cggattctag
    14101 aaattgactg acatctttct aagatcgttc agtcttctgg ttctttcgga gtaataatga
    14161 aagatatttc aataacgcgg gcaggttatt tattgtcgga ctaaataagt gtcaaaaagt
    14221 aaatccagct tcaataatat ttaggaataa gctttggatt tctccatagt ttgtgctata
    14281 atcagtcaag cgaaagagaa actattcgaa aggaggcgta atagaatgat gatgagactt
    14341 aaaaccggat ttttcaccac ccttaacagg gtctccggtg atgccgcatt ctgccacgcc
    14401 ttcatccgtc gtgtgaggta taaagacgta taaccataga cgatgtttga aggaccgtgg
    14461 cgagtaacag ccacggtcct tttttttatc aaccatttct ccaggaggtg gagttatgtc
    14521 agttaaggta gaggtcgaaa gaatcgagaa agtggagcag aagaagattc agagaaagca
    14581 gagagagaac gaggtttttg gtccttactg tttctggctt tgaggaatga aacggggacc
    14641 atgaaacccg gctcgaaagt acagggggtg aaacaatgaa aatagcgtta agcggagaga
    14701 ttagaaagga ccgtattagt tgtagtgttg tacttccacc cttcatagat aaaccggata
    14761 gtctcatgga gtttccccag gatagtcgcc aggaggtggc gtgatgaaaa agatttatag
    14821 aggttcgaga gagattttca tagatggaat agttgaaaga cagaaagttg agatcgaaag
    14881 caagttcagc agacctcatt atctttgatg gaggtgcgaa atggccgacg cgcttttcgc
    14941 aaataacgta gtcaagtttt tcaaaaagag caagaaagag atcaaagcgc taaagggagt
    15001 aagtttgagg ataaaagagg gagagatcta cggactttta gggccgaacg gctcgggtaa
    15061 atcgacgttc atcaggatcg cttcaactct tttgataccc gacaggggca gcgtatccgt
    15121 cttcggacat gatgtggaaa gagagccggg aatcgttcaa aagctcatca acagggtttc
    15181 cgttgaggcg agtttcttca gaaaactgtc agccatagag aacctggcct tctcggccgg
    15241 tctctatggc atttcgaaga aagaagccat aaagaagatc tactatcttt ccgaacgttt
    15301 cggtctggat gagaagagat tgaaagatcc gctggaagac ttttcaagag gtatgcaaca
    15361 gaaagtcgcc atcgtcaggg ccttcatgac cgaaccacga atgcttcttc tagacgaacc
    15421 gaccacggga ctcgacccgc gagccaagaa ggatgtacag tcactcataa gagacgtgaa
    15481 atcaaatatg aacacgacca tgctcctgac cacccacgac atgcaggaag ccgaaaagct
    15541 ctgtgattat gtgggaataa ttcacctggg caagatcgtt gcagaaggga agccgcagga
    15601 gttgaagaac cagctcagag ggaaggttga caacccgacc ttcgaggatg tcttcatgga
    15661 atacacggga atgtctcttg aggatgcgca gtaccgggag gtagaaacgg catgagttcg
    15721 caggcacttg taagagagct gagggcctcg tgggccttta tcgagagaaa cttcaatttc
    15781 accaagagat actggaaatg ggaactggtc ttttttgtgt acacgatagc caactccgta
    15841 accatgggtt tcataggtaa aggtatcgaa gccttctccg gaacgaaact ggatacgagt
    15901 tatcttatac tgtacatgct tttaggatcg atcctctggg gttatctttc catactcttc
    15961 gagatagttg ccgagacggt tgcctgggag cgctgggaag agactataga gtacaccttc
    16021 atggcccctg tgagaagagc gacccacctt ttgagtacct gcggttacgc catactgtac
    16081 gggattttaa ggagcggttt gattctgctg gcggtatcga tcttcttcga tctgaatctg
    16141 gagaaggcca atctgctttc cgcggtcggt atcctggcgg tatccagctt ctctttcgtg
    16201 ggtctgggga tagtcgcggc cgtattgccg ctgatatcgc ccgagaaggg agcccaggta
    16261 gttcacatat tccaggcgct ccttctgatg ttctcggggg tctattacga agtcgacgtg
    16321 ctgccgcagt ggatgcagca ggtcgggagg ttgtctcccg cgacatatgc cttgagagga
    16381 atgagatcgt cgatactcga aggcaagggc atgatcgaaa tgtggggaga tatatggcct
    16441 ttgatccttc tgggagggtt actccttccg tcggggctgt tatttttcag gaagatggag
    16501 ggctgggcca aacggaaggg cgttctgaag agaagcggat agcgatgaga ggattgattg
    16561 agaaacctat tgcgaaaacg tgaacttctc ttcaaacagc tggatacagg cctggacgac
    16621 ttttctgtcg aaaaggatat cgactccggc cgaaatctcg ttcaaagcct tcttgagacc
    16681 caacgaagac aggtatggcc tgtgtgaaga catggcttca accacgtctg ccacagctat
    16741 tatcctggcc tccagagtta tttcatctcc cttgaggcca tcgggataac ccgatccatc
    16801 cagcctttcg tggtgctgtt taacgatagt ggcgatgggc aggtcgaagc tcatttcctt
    16861 cagcagatcg tagcccgtta cgggatgcag cttcactaga tcgaactcca tcttcgaaag
    16921 ctttccgggc tttgagagaa tctcgcccgg aatcgagatt ttgccgatat cgtgaagcat
    16981 agacgcgatc cttatgagct gaactctgct ttctccgaga ttcagtcttt ccgctatggc
    17041 gcacgccagc tggctcactc tcctctggtg accggcggtg tacgggtctc taatctcaac
    17101 cattctcaag agtgtgttga gtagagtgag catactcttc ctgatgatct cttcgttgtt
    17161 tttacgctcg gtgttctccc tgacgatgtc gagtattact tcttctcccc tgagcattat
    17221 gaaacgtgcg cttacctcga ccggaaagat cgaaccatcc ttttttctgt gtaaagtctc
    17281 gtactttgcc ccgtcttcgt aagcgctcag aagttgctcg tctatagttg aaacactccc
    17341 gggagccgtg aagtcgggaa ttgatagttt gagcgcctct tctctggagt agccgtaggt
    17401 ctttatcacg gccctgttga catccagcac tctcaaatcg caagccctga ctatgaatat
    17461 catatcgttg gcgttatgga agataacgcc atacttctta atgttatcca ggtactcctg
    17521 actgagagtg atatctctta tataaccccc gatacctgta tcgctgttag caagcttcac
    17581 aggaaatttg taagtttcat agactttgtt tgccgttctc tccttggtta cgataagacg
    17641 gttgtgttct acggccttca tgtcgctttc acggcacttg ctggccactt cgtactgcat
    17701 caactcgaaa tcggtctttc caattatttc atcgatactc tttttaaaat atttcgagag
    17761 agccctgttg gcgataagat atttgaactc tttatccttt aaaaagacca tatccggtgt
    17821 tgattctatg aacaccatgt gttcgtgctg aagacgatgg tatttaccga ggtttttgag
    17881 ccagcgttcg atatccactt cgtttctgcc gatgtttttg tagaattccg gagggagat
//
LOCUS       DAOOLY010000014        17886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3265_length_17886_cov_5.114407, whole genome shotgun sequence.
ACCESSION   DAOOLY010000014 DAOOLY010000000
VERSION     DAOOLY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_17886_cov_5.114407"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLD15_01465"
     CDS             complement(<1..427)
                     /locus_tag="PLD15_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB62595.1"
                     /translation="MSAKILVVDDSASDRFIIEKMLREYSVLTACDGVEAMRQINENE
                     DIDLIILDLNMPGMDGFEVLNELKSDDRYKRLRAIILTNYDELDNEIKGLKLGAVDYI
                     RKPINMHSLKARIEIHIELLHVQRLLEQKLNEQVITFDTV"
     gene            complement(472..3348)
                     /locus_tag="PLD15_01470"
     CDS             complement(472..3348)
                     /locus_tag="PLD15_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB62596.1"
                     /translation="MRKLLAFLIVFVGTALMITGSCYAIDKSITWTEDEIAFINEHPV
                     IRLGVDPAFVPFEFIDKDGKYKGIAADLLQLIGEKTGLRFEVVQGLTWPEAYDMALAG
                     EVDALPAISRTDERELHFLFSEPYYYFKRVIVTRDTDTAISGIEDLEGLTVAVQRNSS
                     HHSYLLYYPKINLSLYDSVEAAITAVANGTERAFVGNLATTNYLIRANGLTNLRFTAF
                     EADRQLTLNFAVRKDWPLLVSIFNKALNTITEEEKIAINNRWIDLGSETDYGPIIRTI
                     SIFVVLAAIVMVVSFYWIIRLRNEIKKRELIQIDLEKAKREAEDANEFKSSFMARMSH
                     EIRTPLNAITGMAYLLKKTNISLTQTMYVDRITQASNNMLSIINDILDYSKIEAGKVE
                     LEIASFSMDQVIQDVVNIVSYKIEEQGIGFRLSKDPLVPNWFFGDQKRIEQILLNILN
                     NAAKFTASGEVSLDVRLVAREKEKYHLSFSIKDTGIGMTEEQVKKLFVPFTQGDSSIN
                     RRFGGSGLGLSIVKNLLDMMGGNIQLFSTPGEGSTFIIQLSLSIDKEKEELYAKTFSA
                     THFKNVRTLVLERTGASMNLLESYLGAFGMHCELTSSQASALSMLEAADGKFAKPFDL
                     FIIDYDTPAGGGFEFIESIRKNPRIARMPKLIVLLPMMREDLFDRLDDYGIDMGIGKP
                     IIPSILLNGILDIFNLKAVSASQPLEKKAGPARLDGSHRVLLAEDNKTNQLIAKSILE
                     QVGIESILAGDGRAAVELYRQHRNSIDLILMDLHMPVMNGYEAAREIRKISADVPIVA
                     MTADVILGVREKCEQSGIYHYISKPFDPDRFIQTVREIIQKSEEKSVQEPTVLDRSAG
                     LKNMGGDLEVYRQVLDAYHRENRDTPDTLGKAIEERRYPDAVQIVHKVKSSSGSIGAR
                     ALYEVSVELQKALSDSSEEEIPPLKERFVSLLGKLLEEIGRFQN"
     gene            3725..4630
                     /locus_tag="PLD15_01475"
     CDS             3725..4630
                     /locus_tag="PLD15_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPB62597.1"
                     /translation="MYEWHENVQQIIELIERNLDDSPSLSEVGESLFYSPFYCTKKFH
                     ALTGMRLRDYIRLRKICKAALELRDTGKSIAEIALDRGFSSQAAFSRSFKKAYSVSPK
                     EYRSSPRPIPLLLKRIVYDPCVLGIKRRCNLSEKELEKIEVKFEKLHAHRFIGIRNVD
                     AKGYFHFWELQESIPGQDCHTVTGLLESIPSLHGQIGGWYFEGGKRGYLYGIEVPIDY
                     SGEVPEGMECTTIQESEYIVFYYPPYVFEENNDAVMSTVNDLAWSWNPSDSGYEWNIE
                     NPIYQRSDPEKYGYAVCRPVRPLKK"
     gene            4836..5849
                     /locus_tag="PLD15_01480"
     CDS             4836..5849
                     /locus_tag="PLD15_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB62598.1"
                     /translation="MIRGKQDDYPTLSEILQSYNVLQNKVENFINMRPGFFFVFSAER
                     IEGSRGPYYACTVGDSKSRVESKAWIGDSGVIEPRAGHIALADYMRDEKYGLSIKVKR
                     NFTLDEMEAYSAGSTTQLIPVVRDIAALRSEIQRLIESVKNGFLRELLEGLLGENGSC
                     REFFDSPAAKTYHHALIGGLAGHSFQVARYALGIYDLSPSRESIDRDMLVTAALLHDI
                     GKVKTYSTDDYAFEYTDRGQLEDHIAIGVRILEREIVKIDGFPETLASELIHAVISHH
                     GELQYGSPVTPKTREAFILSLCDNLDARLDHFESQAAATPEDSSWTEYSRMFQSRLYR
                     GNH"
     gene            5982..7793
                     /gene="lepA"
                     /locus_tag="PLD15_01485"
     CDS             5982..7793
                     /gene="lepA"
                     /locus_tag="PLD15_01485"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPB62599.1"
                     /translation="MFGRDLIRNISIIAHIDHGKTTLVDRFLDITGTIEHRRMKDQFM
                     DSMDIERERGITIKSKPVKLNYTARNGKTYEINIIDTPGHVDFNYEVSRSLAACEGAI
                     LLVDASQGVEAQTVGNTYLAIENDLELIPVLNKIDIPNANIEETLAEIVDLIGYKAEE
                     CLLASAKTGEGVVEILEAVVERVPPPSGNTSAPLKALIFDAIYDKYRGVVVHVRVFEG
                     SVRAGDRIMMMSSREIFEVIEVGYFLPQMVPTDSIDAGEVGYIIAVIKEVASAKIGDT
                     ITGATSSTPEALPGYKVAKPMVYAGMFPGMPEYYEELRKALDKFKLNDAALVFEPENS
                     PALGFGFRVGFLGLLHMDVVRERLEREFDITCILTAPNVVYRVTMSDGTVQEITNPAS
                     FPEPGEFAKVEEPFVELSIITPSDYMGNLIGFITTEKRGEFKAVENAGKNRVVMKFVT
                     PLSEIMFDFFDRMKALSRGYASMDYDIIGYRESNLVKVTILVNKDTVDSLSFIVHVDK
                     EYAVAKKVVDKLSELIPQHQFQIPIQARARGRIIARSDIKALRKDVLAKCYGGDVTRK
                     MKLLEKQKEGKKRMREIGQVTIPQNAFLAILRIGDEE"
     gene            7811..8680
                     /locus_tag="PLD15_01490"
     CDS             7811..8680
                     /locus_tag="PLD15_01490"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPB62600.1"
                     /translation="MIKLGAHMSTSKGFDKVPGDTSKMGGNTFQIFPHSPRMWRASLP
                     EDKIARAFKDEMKAKGIDPYSCMVHSGYLINLASSSEEVWDKSVTLLSIEMKITAALG
                     LKYLNFHPGSHLGDGLQTGIDRIVRGIDIVLGENQDNDVMLLLENVAAKGNHIGSSFE
                     ELKSIIDGSSGPSRIGITYDTCHGFDSGFDIRTIDGTKFLIESIDSTVGYEKLKMVHL
                     NDSKFPLGAGKDRHEVIGKGYIGMDGGFREFLSCEEIQQKPWLLETPGEDADHAEEIK
                     YIRELLNNRENIL"
     gene            8677..10257
                     /locus_tag="PLD15_01495"
     CDS             8677..10257
                     /locus_tag="PLD15_01495"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPB62601.1"
                     /translation="MKKKYVVVTGGVLSGIGKGILSASIARVMKECGVEVNTLKIDPY
                     LNIDAGTMNPNQHGEVFVTEDGYEADLDLGHYERFLGKDMRRENNMTAGQIFKAIIDK
                     EREGSFLGATVQMVPHVTEEIKGRIRRIPGELIMIEIGGTVGDIEGEIFLEAIRELSV
                     EEGSENFLFVHVTYVPYLRVTSEFKTKPTQQSVQLLRRIGIQPQMIAVRSEEPVEENE
                     LRKIALFGGVEREMVFNLPDSKNVYDVPSIVYSYGIHRKIATRLNLEIMERFEWDYPR
                     NFIPSKIAIIGKYLGTDDAYKSISESICLCGASKPEVLDSQVFEDMTQEQIKNTLAEY
                     EGLIIPGGFGKRGIEGKIAVIRYARENNVPILGICLGMQLMVIEYARNVAGLEGANST
                     EFDPATPYPVIDIMEEQKKILKLGGTMRLGAQETPIKVGTLLMEAYGASPVHERHRHR
                     YEVNYEDFRDLFSEPDENLSGRLVISSKAAFVEAIELPGRDFFLGIQYHPEFGSKVGR
                     PSPLFRLFVERVRGRKKD"
     gene            10263..11099
                     /gene="folP"
                     /locus_tag="PLD15_01500"
     CDS             10263..11099
                     /gene="folP"
                     /locus_tag="PLD15_01500"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB62602.1"
                     /translation="MDFRNGRSLDLSSPVIMGIINTTPDSFYPGSRVPDPRTAVERAM
                     EMLDSGASIIDIGGESSRPGAGAIDWQEEVRRVVPVVRGIRERRPEAIISVDTYNCNT
                     ASQALSKGADIVNDITGLIDPAMIEVVAKNGAAVVIMHMKGTPSTMSRLTDYSDVVSQ
                     VKEQLLERCEKAVAGGVRKDGIILDPGIGFAKNALQSLEILKNITDFTCLKYPVLVGH
                     SRKSFMGYVSGLPVENRLEETLAVSTYLYLKGVKILRVHDVEEHRRIFDILKLMDKST
                     EQ"
     gene            11115..12443
                     /locus_tag="PLD15_01505"
     CDS             11115..12443
                     /locus_tag="PLD15_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62603.1"
                     /translation="MSYNRRSKKYWRETWKAYLFLLPSLAILGVFTFWPIGFSLVLSF
                     FKWDYTSASRYFIGLDNYKELFRISYPVQMNLLYSIINMAVYAVATLVLVQMVHFLLC
                     GASKYDKPEKEHATLYALFAGVLAGYVVLRNLSDGVTMAGILYGLAMAAFALVIFLRR
                     RGSIQSVKMKSSQWVTLILFIGIYYLLSRVLLKESVDFLQYFVLAKESSDFLKSIFNT
                     IYYVVLSVPTQIVLALLIAVLLNKSIKLRSLFRTAYFIPFVTSVVAVSLVWQWMFNDQ
                     FGLLNYILSWFNLPKIAWLKEEVWTIPTIAIVSVWQHVGYTAVIFLAGLQNIDRSYYE
                     AADVDGASSWQKFKFITWPLLSGTTFFIMIITMIGAFKVFSQIFILYQGLPGPVNKSG
                     LTLVYYVFDAFYNQQRMGVASAAAYILFMIILLLTMVQLYVGKKRVHYEG"
     gene            12453..14864
                     /locus_tag="PLD15_01510"
     CDS             12453..14864
                     /locus_tag="PLD15_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB62604.1"
                     /translation="MNTSSTRSISKIFIYVLIIGGAFLMLVPFAWMVDTSFKAPSEVS
                     SWPPVWTTKNAASSFQFKTRIKQKSSSTSVDLSTLSLSEFRNFAALLESTGSFDTLTV
                     SLDDDPIVRGEIEILLLSNNGLPAFYAVDLDMARYGELAEKFLSLKGAFPQTYSGELE
                     KLNTDDPGTFFDGFFGVSIYQDTGFIRRVVLVNSLKSLYSSTIDRLLQSAETTFKDLP
                     IDNDSTKQLKAELREEIRDIFSDTVSAIETAMAGLDSYRTGTSGVTSLPELATVISLM
                     RENISKIEADAQESLSLARSKASSDPRLTMLVNQFERSLESISDGPIRWTYLMDFYVE
                     ARNFYDGVQTVSLSSGSIVGRIRTDEQVHGLVIKAIDTWKADPATKEYLKSTLTPSNI
                     RNAVTILLTYLDGNYRDSLAGHFSSPRTVLETVNEAMNFLRTSLLVAADPSLDSRVRQ
                     IMTSESSYQELESVIREVATTAGQSITYGKISAGLSRFAGESDPEVFARFIRRRWEET
                     EMVGSFSKMHSNIFTELDLVSKPDEVEKVFLRGLISPSGSKSFDIKLKGIPAVWFKDD
                     VSSAKANFTFAETIRNFFQNYVTAWNAAPFGTYYFNTVFVAIVTTFAEIIFAAMAAFA
                     FAKMEFFGKNLIFTLFLATMMVPGEVLLVPNYITLTALGWIDSYYALIVPWVVSVFAI
                     FLLRQHFMTIPNELYDAAMIDGLTKWRFLWTVMVPLSKPAVITGALLKFVGSWNSFLW
                     VLIVTKSPEIRTLPVGLQNFSSATGSDYHLLMAAATFSVIPVVVLFLLTQKYFIAGIA
                     RSGLK"
     gene            14921..15100
                     /locus_tag="PLD15_01515"
     CDS             14921..15100
                     /locus_tag="PLD15_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62605.1"
                     /translation="MLSLLLITNYNAIAKLKEEKQRLETMEKRYNELEKQIQIREAEL
                     ELLRKLMQEKGAGVY"
     gene            15100..15882
                     /locus_tag="PLD15_01520"
     CDS             15100..15882
                     /locus_tag="PLD15_01520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB62606.1"
                     /translation="MNYIVTTSYKPSGEEVTKARNLAGEMGVRYVSRNRLKEYEKETA
                     LDFYYVFDKNGQLVIRSGEEVFFFHPGMSKVRLKNIKLQDSDYLIKSMELAGTELVLD
                     TTFGLGNEALLIANFLPGGKVVGLEASEHIFRVVSHGLKNYPYEDDWTREAAGRIELY
                     NRDLREFVRNCGEGSYDIVYCDPMFDRPQYRSHSINPLRPFAVYDRINREDVDWMIRA
                     ARRKFILKSRTRDTLFQELGIEFDRLWGSKKSGVLYGVIDKR"
     gene            15879..16799
                     /gene="miaA"
                     /locus_tag="PLD15_01525"
     CDS             15879..16799
                     /gene="miaA"
                     /locus_tag="PLD15_01525"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPB62607.1"
                     /translation="MIPVILGPTAVGKTALLLELAMRLPIEVVSVDSRQIYRHMDVGT
                     AKPTKRERESLRHWLIDIRDPDEDFDVKTFRELALEAIEDITSRGKIPVLAGGTGLYA
                     EALMKGLADVPGRNESVREALAELERASPGSLRKMLKALDSQAYGRLHENDLKRSIRY
                     LEAFFLTGKPLSEVQNEKTVSDRYKVIVLNRSRSELHGRIELRVEEMYRRGLMEETRE
                     LLVMGYTANINALKTIGYAEMVQCIRGLISPQEALEKIKCNTRRYARRQIIWFRRYSG
                     LEIDLSNLTESCAVSTLERTILLVWGGNYG"
     gene            16792..17034
                     /gene="hfq"
                     /locus_tag="PLD15_01530"
     CDS             16792..17034
                     /gene="hfq"
                     /locus_tag="PLD15_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPB62608.1"
                     /translation="MAEKFNLQDRFLNLLRVNKVEVKMYLEGGFQTKGMVKSFDNFTV
                     LLEDGQEQTLVYKHAIKMMVPQKYVRLTNTTGKKED"
     gene            17099..>17886
                     /gene="hflX"
                     /locus_tag="PLD15_01535"
     CDS             17099..>17886
                     /gene="hflX"
                     /locus_tag="PLD15_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPB62609.1"
                     /translation="MVGELNELARTANYNIIETVIQNLDYPDPRHYLGRGKVDFARRM
                     LESYGAEIVITRHELSPSQAFNLEKHLGIKVMDRTQLILKIFADHATTKEGKLEVELA
                     SLKYQLPRLKGYGRMLSQTGGGIGTRGPGEKKLEIDRRLAQEKITHLKREIEELKRQR
                     EVSRKRRVGSASPLVSFVGYTNVGKSSLVSLLSGENLLIQDKLFATLDTRVRRVRLIS
                     GLQILVSDTVGFIRELPHELMESFRATLDEVRFSDLLVIVSDAS"
BASE COUNT         4463 a         4454 c         4853 g         4116 t
ORIGIN      
        1 aaaccgtatc aaacgttatc acctgttcat tcagtttctg ctcgagcagt cgctggacat
       61 gaagcaactc gatgtggatt tcgattctgg cctttaggga atgcatgttg ataggctttc
      121 ttatatagtc cacagcaccg agtttcaggc cttttatttc gttgtccagt tcatcgtagt
      181 tagtcagaat gatcgcacgc agcctcttgt acctatcgtc tgacttcagt tcgttcagaa
      241 cctcgaaccc atccatgccg ggcatgttca aatcgagtat gatcaggtcg atatcctcat
      301 tttcgttaat ctgcctcatg gcttcaacac cgtcgcaggc ggtgaggacg ctgtactcac
      361 gcagcatctt ttcgatgatg aatcggtctg aagcagaatc gtcgacaacc agtattttcg
      421 cagacatcgt tcattccccc tcgctataag aaacaaatgc cgaaacatat ctcaattttg
      481 aaaccgcccg atctcttcca gaagctttcc gagcaaggag acgaatctct ccttaagagg
      541 cggtatctcc tcttccgaac tgtcgctcaa agccttttgc agttccaccg aaacttcgta
      601 cagagccctc gcgccgatac tgccggagct gctcttgacc ttatggacga tctgtacggc
      661 atccggatat ctcctctctt cgatagcctt accgagcgta tccggcgtgt cccggttctc
      721 ccggtgatac gcgtccaata cctggcggta cacttccaga tcgccgccca tgtttttcag
      781 tccggccgat cgatccagaa cggtcggctc ctgaacactc ttttcctcgc tcttctggat
      841 gatttccctg acggtctgga taaagcgatc cggatcgaag ggcttgctta tatagtggta
      901 aattccactc tgctcgcatt tttcccgaac tccaagaatg acatccgcgg tcatcgccac
      961 tatgggtaca tcggccgata tctttctgat ttcccgcgcc gcttcatagc cgttcataac
     1021 gggcatatgc aaatccatca aaatcaagtc gatgctattc ctgtgttgcc tgtaaagctc
     1081 gacggcggcc ctgccgtcgc cggccaggat agattcgatg cccacctgtt cgaggataga
     1141 tttcgcaatt aactgattgg tcttgttatc ttcggccaga agcactcggt gggatccgtc
     1201 gagtcttgcg gggccggctt tcttctccaa cggttgagag gccgacaccg ccttcaaatt
     1261 gaaaatgtcg agaatcccgt tcaacagaat cgaagggatt attggcttgc cgatgcccat
     1321 gtcgattcca tagtcgtcga gcctgtcgaa gagatcctcc ctcatcatcg gcaaaagcac
     1381 gatgagcttc ggcattctgg cgatcctcgg gtttttgcgt atcgactcta tgaattcgaa
     1441 gccgcctccg gcaggtgtgt cgtagtcgat gatgaacaga tcgaagggct tggcgaattt
     1501 accgtcggct gcttcaagca tgctcaacgc gctcgcctgg gaactggtga gctcgcaatg
     1561 cattccgaag gcgccgagat agctctccag aaggttcatg ctcgcccccg tcctttccag
     1621 gacaagcgtc ctgacgttct tgaaatgagt cgccgagaag gtctttgcgt aaagctcctc
     1681 tttctctttg tcgatactca gagaaagctg gattatgaac gtggagcctt ccccgggggt
     1741 gctgaagagc tgtatatttc cccccatcat atccaggagg ttcttgacga tagacagccc
     1801 caatccggaa cctccgaagc gccggttgat actgctgtct ccctgcgtaa aaggtacgaa
     1861 gagctttttc acctgttctt cggtcatgcc gatgccggtg tcttttatcg agaaggagag
     1921 atggtatttt tccttttccc tggccacgag cctcacgtcc agcgagactt cgccggaagc
     1981 ggtgaatttc gcggcgttgt tgaggatgtt gaggaggatc tgctcgattc gcttctggtc
     2041 gccgaagaac caatttggaa cgaggggatc tttcgaaagc ctgaagccga tcccctgctc
     2101 ttcgatcttg taagacacga tgttcacgac gtcctgaatg acctggtcca tgctgaaaga
     2161 agcgatctcc agctccacct tgccggcttc gatcttagag taatccagga tatcgttgat
     2221 tatgctcagc atattgttcg atgcctgagt gattcgatcg acatacatgg tctgcgtcag
     2281 ggagatattg gtctttttca gcaggtaagc cattcccgta atggcgttga gcggtgtccg
     2341 gatttcgtgg gacattctcg ccatgaagct ggacttgaat tcgttcgcat cttcggcttc
     2401 tcgttttgcc ttttccagat ctatctgaat gagttcgcgc ttcttaattt catttctcaa
     2461 tctaatgatc cagtagaacg agaccaccat cacgattgca gccagtacca caaagatcga
     2521 aatggtccgg atgatcggtc catagtcggt ttccgatcct agatcgatcc acctgttgtt
     2581 gatggcgatc ttctcttctt ccgtgatcgt attcaacgct ttgttgaaga tgctcacaag
     2641 aaggggccag tctttgcgaa cggcaaagtt caaagtcagc tgtctatcgg cttcgaatgc
     2701 ggtgaaccgt aagttcgtca atccgttggc ccgtataaga taattagtgg tggcgaggtt
     2761 cccgacgaaa gccctttccg tgccgttggc gactgccgtg atcgccgctt ccacagagtc
     2821 gtagagactc agattgattt ttggataata cagcaggtaa ctgtgatggg agctgttcct
     2881 ctgcacggcc accgtcaacc cttcaagatc ttcaattccc gagattgccg tatcggtgtc
     2941 cctggtcacg attaccctct tgaagtagta atacggttcg gagaaaagaa aatgcagttc
     3001 tctttcgtcg gttctggata tcgccggcag agcgtccacc tctcccgcca gagccatatc
     3061 gtaggcttcc ggccaggtca acccctgaac aacctcgaac cgcaagccgg ttttctcgcc
     3121 gatcagctgc aacaaatcgg cagcgattcc cttgtacttt ccatccttgt ctatgaactc
     3181 gaacggaacg aacgccggat cgacgccgag tcgaatgaca ggatgttcgt tgatgaaggc
     3241 aatttcatcc tccgtccagg tgatgctctt gtctatcgca taacaggagc ctgtaatcat
     3301 tagagccgta ccgacaaaaa cgatcaaaaa ggccaagagc tttctcatga gcgctcacct
     3361 tcataatatc gactttcagt ctcgactcct agctatctat ttccgggtgt gccccggaaa
     3421 ggggcaccaa aaagccgaaa ggaagcctaa aaagatgtta ccataaaaaa tggagaaaaa
     3481 ggaaattatt aaggggaatt taatatagag aactgttaaa aaacagtgtg aacgttacga
     3541 gaaaagcgga acagagatcc aatcgaatga gtacctgagg agactaacaa aagtgaatca
     3601 ccgtaaacgt atcataataa ccttatacat gaaatatgct tatacaatga ggttagctta
     3661 aagtgcaact cagatcaaga aattgcctct cttttactgt atatttttcc cgggagggat
     3721 cgctgtgtat gaatggcatg agaacgttca gcagatcata gaactcatcg agcggaatct
     3781 cgatgatagt ccttctcttt ccgaagtggg cgagagtctt ttctattcgc ctttttactg
     3841 cacaaagaag tttcacgccc tgaccgggat gcgactgagg gattacatca ggcttcgaaa
     3901 gatctgcaag gcggcgctgg agttgcgcga taccgggaaa tcaatcgccg agatagctct
     3961 tgatagaggc ttctcctcgc aagcagcttt cagtagatcg ttcaagaagg cttactcggt
     4021 ttcgccgaag gagtacagat cctctccaag accgatcccc cttctgctga aaaggattgt
     4081 ctatgatccc tgtgttctag gtatcaaaag gaggtgcaac ttgagcgaga aagaactcga
     4141 aaagatcgaa gtgaagttcg agaaactgca cgcacacagg tttatcggga tcagaaacgt
     4201 tgacgcaaag ggatatttcc acttctggga gcttcaggaa agcattcccg gtcaggactg
     4261 tcacaccgtg acaggtctgc tggagagcat cccgagcctt catggccaga tcggcggctg
     4321 gtattttgaa ggcggtaagc gtggctacct gtatgggatc gaagtgccta tcgattacag
     4381 cggagaagta cctgaaggta tggagtgcac tactattcaa gagagcgaat acatcgtctt
     4441 ttattacccg ccatatgtat ttgaagagaa caacgatgcc gtcatgtcga ccgtgaacga
     4501 tcttgcatgg agctggaatc cgtcagattc cggctacgaa tggaacattg aaaatccgat
     4561 ctaccagaga agcgatccgg aaaaatatgg ctatgccgtc tgccgtcccg tgagaccttt
     4621 gaaaaagtag aaatagtgaa atagaagtag taatgaaagc ccctgattcc aggggttttc
     4681 tgattcttac acttatttcg ttcgcgatga taggtaattt cctccattcg cccttctctg
     4741 aagaagggtt tgtgaacata agtcccatcg ttcgcagtct attattggta aagacttaac
     4801 agaaacccgg agtggccgaa aggagagaaa gcaaaatgat aaggggaaag caggacgatt
     4861 atcctacact gtcggagata ctccagtctt acaacgtgct ccagaacaag gttgagaatt
     4921 ttatcaacat gaggccgggc tttttcttcg tgttttcggc cgaaaggatc gaagggagtc
     4981 gcggcccgta ctacgcctgc accgttggcg attcgaagag cagagtcgag tccaaggcat
     5041 ggataggcga ctctggagtt atagagccgc gtgcaggcca tatagcgctg gccgattata
     5101 tgcgcgacga gaagtacggg ctgagtataa aggtgaagag gaatttcaca ctcgatgaga
     5161 tggaagctta cagcgccggc tctaccaccc agctcatacc tgtcgttcgc gatatcgccg
     5221 ctctcagatc cgagatacaa cggctgatcg agagcgtgaa gaacgggttt ttgagagaat
     5281 tgcttgaggg attgctgggc gagaatggtt cctgccgcga gtttttcgac tccccggcag
     5341 ccaagactta tcaccacgcc cttataggag gcctcgccgg tcacagtttc caggtggcca
     5401 ggtatgcgct gggcatatac gacctttcgc cttcgcgaga gagtatcgac agggatatgc
     5461 tggtgacggc ggcgcttctg cacgatatag gaaaggtgaa aacctattcg accgacgatt
     5521 acgccttcga atacaccgat cgcggtcagc tcgaggacca catagcgata ggagtgagga
     5581 tcctcgaaag ggagatcgtg aagatcgacg gcttccccga gactctggcc agcgagctga
     5641 tccatgcggt tataagccac cacggggagt tgcaatacgg ctctcccgtg acaccgaaga
     5701 ctagggaggc cttcattctg tcgctgtgcg acaacctgga cgcgaggctg gaccatttcg
     5761 agtcccaggc cgccgcgacg ccggaagact cttcctggac cgagtattcg cgaatgttcc
     5821 agagcagact ctacagagga aaccactgag ataacgtctt gagctttatc ctgacgtccg
     5881 acggttcttt ttctacacat attactacat tttcgcggga ggttaaaact accgcggtca
     5941 tcgcatgtta caattcttct tgatcggcgg gagggaattt catgtttggt agagatctga
     6001 taagaaatat atcgattatc gcccatatcg accacggaaa gactacgctg gtcgatcgtt
     6061 ttctggatat aaccggcact atcgaacacc ggcgcatgaa ggaccagttc atggattcga
     6121 tggacatcga gcgcgagagg ggaataacga ttaagtccaa acccgtgaag ctgaattaca
     6181 cggcccggaa cggcaagact tacgagataa acataataga cacgcccggc cacgtcgatt
     6241 tcaattacga ggtttcgagg agtctggccg cctgcgaggg ggcgattctt cttgtggacg
     6301 cctcgcaagg tgttgaagcc cagacggtgg gaaacactta tctggccata gagaacgacc
     6361 tcgagctgat accggttctg aacaaaattg atattccgaa cgcaaatatc gaggagacgc
     6421 tggccgagat agtcgatttg atcggctata aagccgagga atgtctgctg gcgagcgcca
     6481 aaacgggaga gggcgtcgtg gagatactcg aggcagtggt cgagcgcgtc ccgccgccgt
     6541 cgggtaatac ttcagctccg ctcaaggcgc tgatcttcga cgcgatttac gacaagtaca
     6601 ggggtgtggt tgttcacgtc agagttttcg aaggttcggt cagggcaggc gacagaatca
     6661 tgatgatgtc ctcccgtgaa atcttcgagg ttatagaggt tggttacttc cttccgcaga
     6721 tggtcccaac agattctatc gacgccggag aggtcggtta catcatagcc gttatcaaag
     6781 aagtggcaag cgccaaaatc ggcgatacga tcacaggcgc caccagctcc actcccgagg
     6841 cccttcccgg ttacaaagtc gccaagccca tggtttatgc cggcatgttt cccggcatgc
     6901 ccgagtacta cgaagagctg cgaaaggctc tcgacaagtt caagctgaac gatgcagccc
     6961 tggtcttcga gccggaaaac tccccggcgc tgggctttgg cttcagagtc ggcttcctcg
     7021 gcttgctcca tatggatgtg gtgagggaga gactcgagag ggagttcgat atcacctgta
     7081 tactgacggc gccgaacgta gtttacaggg taaccatgag cgacggaacc gttcaagaga
     7141 ttaccaaccc ggcaagtttt cccgaaccgg gagagttcgc aaaggtcgaa gagcccttcg
     7201 tggagctttc tataatcact ccatccgatt acatgggcaa cctcatcggt ttcattacga
     7261 ccgaaaagag gggcgagttc aaggccgtcg agaacgccgg caaaaacagg gtggtcatga
     7321 agttcgtgac gcccctctcg gagataatgt tcgacttctt cgacaggatg aaggcgctct
     7381 caagaggata cgcctcgatg gattacgata tcatcggcta cagagaatcg aacctggtca
     7441 aggtgaccat actcgtcaac aaggacaccg tggattccct gtcgttcata gttcatgtag
     7501 acaaggagta cgcggtggcc aagaaggttg tggacaagct gagcgaacta ataccccagc
     7561 atcagttcca gataccgata caggccaggg cccgtggcag gataatcgcc cggagcgata
     7621 taaaggccct caggaaggac gttctggcca agtgctacgg cggggacgtt accaggaaga
     7681 tgaagctgct ggagaaacaa aaagagggaa agaagagaat gagagagata ggtcaggtaa
     7741 ctattcctca gaacgccttc ctcgctatct tgcggatcgg cgatgaagaa tgagagcgcg
     7801 aggtgattcc gtgataaagc tgggcgcgca tatgtcgact tcgaagggct tcgataaagt
     7861 ccccggagat acgtcgaaaa tgggcggaaa cactttccag atcttcccgc acagtcccag
     7921 gatgtggaga gcctcgcttc ccgaggataa gatcgcccgg gctttcaagg acgagatgaa
     7981 agccaaaggc atcgaccctt actcttgcat ggtccactcg ggttatctta taaacctggc
     8041 ttcctccagc gaggaggtat gggataagtc tgtgacgctg ctctcgatcg agatgaagat
     8101 cacggccgct ctggggctga agtatttgaa cttccatccg gggagccatc tgggggacgg
     8161 tctccagacc ggcatagaca ggatagtgag gggaatcgat atagttctgg gcgaaaatca
     8221 ggacaacgac gtgatgcttc ttctggagaa cgtcgccgcc aagggcaacc atataggcag
     8281 tagtttcgaa gagttgaaga gtattataga cgggtcgtcc gggccttcga ggataggcat
     8341 aacctacgat acctgtcacg gtttcgattc cggcttcgat atcaggacta tcgacgggac
     8401 gaagtttctg atagagagca tcgactccac ggtcggatat gagaagttga agatggtaca
     8461 cctcaacgac agtaaatttc ccctgggcgc cggcaaggac agacacgaag tcataggcaa
     8521 gggttatata ggcatggacg ggggattcag ggagttcctc tcctgcgaag agatacagca
     8581 gaagccctgg cttctggaga ccccgggcga agacgccgac cacgcagaag agatcaagta
     8641 tataagagaa ctattgaata atcgggagaa cattctatga agaagaagta cgtggtagta
     8701 acgggaggag tgctcagcgg aatcggaaag ggaatacttt cggcctccat agcccgtgtt
     8761 atgaaggagt gcggggtgga ggtcaacacc ctgaagatag acccatacct caatatagat
     8821 gccggcacga tgaatcccaa ccagcacggg gaggttttcg taacggagga cggctacgaa
     8881 gccgatctag acctcggcca ttacgagagg ttccttggaa aggatatgcg acgcgagaac
     8941 aatatgacgg ccggccagat cttcaaagcc atcatagaca aggagaggga agggagcttc
     9001 ctgggggcga ccgtccagat ggtgccgcac gtgaccgagg agataaaagg caggataaga
     9061 aggattccgg gcgagttgat catgatagag atcggcggaa ctgtgggcga catcgagggc
     9121 gagatctttc tcgaagccat cagggagctc tcggtagagg agggttccga gaactttctc
     9181 ttcgtccacg tgacctacgt accctacctc agggtcacga gcgagttcaa gaccaaaccc
     9241 actcagcagt cggtgcagct cttgaggagg ataggcattc agccgcagat gatcgccgtg
     9301 agatccgagg aaccggttga ggagaacgag cttagaaaga tcgccctctt cggcggcgtc
     9361 gaaagggaga tggtcttcaa tctgcccgac tctaagaacg tttacgatgt cccctcgatc
     9421 gtttactctt acggcattca cagaaagata gccaccaggc tcaacctcga gataatggag
     9481 aggtttgaat gggactaccc tagaaacttc atcccttcga agatcgccat aatcggcaaa
     9541 tatctgggaa cggacgacgc ctacaagagc atatccgaga gtatttgcct gtgtggcgcc
     9601 agcaagccgg aagtcctcga ttcccaggtc ttcgaggata tgacgcagga gcagatcaaa
     9661 aacaccctgg ccgaatacga gggcctgata attcccggcg gtttcggaaa gaggggcata
     9721 gagggaaaga tagccgtgat aaggtacgcg cgcgagaaca acgtacccat tctgggtatc
     9781 tgtctgggta tgcagctaat ggttatagag tacgccagaa atgtggccgg ccttgaaggc
     9841 gctaactcca ccgaattcga tccggcgacg ccctatccgg tgatcgatat aatggaggaa
     9901 cagaagaaga tactcaaact cggcgggacc atgagactgg gcgcacagga gactccaata
     9961 aaggtgggta cgcttctcat ggaggcttac ggggccagtc cggttcacga gaggcacagg
    10021 cacaggtacg aagtcaacta cgaggacttc cgggacctct tcagcgagcc ggacgagaac
    10081 ctttccggca ggttggtgat ctcgtccaag gccgctttcg tggaggccat cgagttgcca
    10141 ggcagggatt tcttcctcgg gattcagtac cacccggagt tcggctcgaa agtgggccgc
    10201 cccagtccgc tgttcagact cttcgtggaa agagtcaggg ggcgaaaaaa agattgacga
    10261 gaatggattt cagaaacggg agatcgctcg atctctcctc acccgtgata atgggtataa
    10321 tcaacaccac acccgattcc ttctaccccg ggagccgggt ccccgatcca cggaccgccg
    10381 tggaaagggc gatggagatg ctcgatagcg gcgcttctat aatcgatata ggcggtgaat
    10441 cttcccgacc cggggcagga gccatcgact ggcaggagga agtacgccgg gtcgttcctg
    10501 tcgtcagggg tatcagggag cgaagaccgg aggcgatcat ctcggtcgat acatacaact
    10561 gcaacacggc ttcgcaggcg ctctcgaagg gagccgacat cgtcaacgac ataaccgggt
    10621 tgattgatcc cgctatgata gaggtggtgg cgaagaacgg agccgcggtc gtaataatgc
    10681 acatgaaggg cactccctcg accatgagcc ggctcaccga ttactccgat gtagtatctc
    10741 aggttaagga gcaactgctt gaacgttgcg aaaaggccgt ggcaggaggg gtgagaaagg
    10801 acgggataat tctcgacccc ggcattggtt tcgccaagaa cgcccttcag agcctggaga
    10861 ttctcaagaa tatcaccgac ttcacctgcc ttaagtaccc ggtgctggtg ggccattcta
    10921 gaaagagctt catgggatac gtctccggtc tgcccgtcga gaatagactc gaggaaactc
    10981 tcgccgtctc cacgtacctc tatctgaagg gagtgaagat attgagagta cacgacgttg
    11041 aagagcaccg aagaatcttt gatatactga agttgatgga caaatccact gagcagtgaa
    11101 tggggggaaa ggttttgtct tacaataggc gatcgaaaaa atactggaga gagacctgga
    11161 aagcctatct gtttctcctg ccttcgctgg cgatccttgg tgtcttcact ttctggccga
    11221 taggcttctc tctcgtatta agttttttca aatgggatta cacttcggcc tctcgctact
    11281 tcataggtct ggacaactac aaggaactct tcaggatctc gtaccccgtt cagatgaacc
    11341 tgctttattc gatcatcaac atggccgtct atgctgttgc gactctggtc ctggttcaga
    11401 tggtccattt ccttctctgc ggcgcgagca agtacgataa acccgagaag gagcatgcaa
    11461 cgctctatgc gctgttcgca ggagttctag ccggttatgt ggttttgagg aacctctcgg
    11521 acggggtgac gatggccggg atactgtacg gactggcgat ggccgccttt gcgctggtaa
    11581 tcttcctgag gcgcagaggt tcgatacaga gcgttaaaat gaagtcgagc cagtgggtaa
    11641 cgctcatact tttcataggg atatactacc ttctttcgag ggttctgttg aaggagagcg
    11701 tcgacttttt gcagtacttc gtgctggcca aggagagctc ggacttcttg aagtcgatct
    11761 tcaacaccat ctattacgtt gtactctcgg ttccgacaca gatagtgctg gccctactga
    11821 tcgccgtcct tctcaacaag agcataaagc tccgctcgct cttcagaacg gcgtatttca
    11881 taccattcgt gacatcggtg gtcgccgttt ccctggtctg gcagtggatg ttcaacgacc
    11941 agttcggcct tctgaactac atcctgtcgt ggttcaacct gccgaagatc gcatggctca
    12001 aggaagaagt ctggacgata cccacgatcg ccatagtctc ggtctggcag cacgtcggct
    12061 atacggccgt gatatttctg gccggtctcc agaacatcga caggtcttac tacgaggcgg
    12121 ccgatgtcga tggagccagt tcctggcaga aattcaagtt catcacctgg ccccttctat
    12181 cgggaacgac cttcttcata atgataataa ctatgatcgg tgccttcaaa gtcttctccc
    12241 agatattcat cctgtaccag ggcttgcccg gacctgtcaa caagagcggg ctcacgctcg
    12301 tttactacgt ctttgacgcc ttttacaacc agcagaggat gggagtggcc agtgcggccg
    12361 cctatattct cttcatgata atccttcttc tcacgatggt gcaactctac gtcgggaaga
    12421 agcgtgtaca ctacgaggga tgaggtggcg ctgtgaatac atcatctacc aggagtattt
    12481 cgaagatttt catatacgtt ctcatcatcg ggggcgcctt cctgatgctg gtacccttcg
    12541 catggatggt ggacacgtcc ttcaaagcgc cgagcgaagt ctcttcctgg ccgccggtct
    12601 ggacgaccaa gaatgccgcc agttccttcc agttcaagac cagaatcaag cagaagagct
    12661 cctcgacgtc tgtggatctt tcaacgctca gtctgagcga attcaggaac ttcgcagccc
    12721 ttctggagag cacgggcagt ttcgatacac tgacggtaag tctcgacgac gatccgatcg
    12781 tgcgcggcga gatcgagatc ctgctcctct cgaacaacgg tttgccggcc ttctatgcgg
    12841 tcgatctcga catggctcga tacggagagc tcgccgagaa gtttctctcc ctgaaaggcg
    12901 ctttcccaca gacctattcc ggcgagctcg aaaagttaaa cacggatgat ccgggaactt
    12961 tcttcgacgg cttcttcggg gtttccatct atcaggatac gggcttcatc aggagagtcg
    13021 tgctggtcaa ttccctgaaa tccctttact catccacgat cgatagactc cttcaatctg
    13081 ccgagacaac gttcaaggac ctcccgatag acaacgactc caccaaacag ctgaaggccg
    13141 aactccggga agagatccgt gatattttca gcgatacggt gtctgcaatt gagacggcca
    13201 tggccggact ggattcatac aggacgggca cttcgggcgt gacgtcactg cccgagctgg
    13261 cgactgtcat aagtctcatg cgcgagaaca tctcgaagat agaagcggat gcacaggagt
    13321 cgctctctct ggccagatcg aaagcatctt cggacccgag gttgacgatg ctcgtgaacc
    13381 agtttgaacg gtcgctggaa agcatctccg acgggccgat tcgctggacc tacctcatgg
    13441 atttctacgt agaggccaga aacttctacg atggcgttca gacagtctcg ctctcgagcg
    13501 gttcgatagt ggggaggata aggaccgatg aacaggtcca cggactggta ataaaggcca
    13561 tcgacacctg gaaggccgat ccggcaacga aggagtatct gaagtcaacc ctgacgccgt
    13621 cgaacataag gaacgccgtc accatacttc taacttacct ggacggaaac tacagagaca
    13681 gtctggcggg ccacttctcc agtccccgga ccgtactgga aacggtcaat gaagccatga
    13741 acttcctgag aacatcgctg cttgtggctg ccgacccctc gctcgattcg agagttcgac
    13801 agatcatgac ctcggaaagc tcctatcagg agctggaatc ggtaataagg gaggttgcaa
    13861 cgacagcggg gcagagtata acttacggca agatctccgc cggactctcc agatttgccg
    13921 gcgaaagcga tcccgaagtc ttcgcccggt tcataagaag aaggtgggag gagaccgaga
    13981 tggtcggcag tttctcgaag atgcactcga acatcttcac cgagctcgat ctcgtttcca
    14041 agcccgacga ggttgaaaag gtcttcctca ggggtttgat ctctccctcg ggttcaaaat
    14101 ccttcgacat aaagctgaaa ggtatcccgg ccgtctggtt caaggacgat gtctcctcgg
    14161 cgaaagcgaa cttcacgttc gccgagacaa taaggaactt tttccagaac tacgtcacgg
    14221 cctggaacgc cgcgccgttc ggaacgtatt atttcaacac ggtcttcgtg gcgatagtca
    14281 ccactttcgc cgagataata ttcgccgcca tggccgcttt cgccttcgcc aagatggagt
    14341 tcttcggcaa gaatctgatc ttcacgctct tcctggcgac gatgatggtc cccggagagg
    14401 ttctactggt accgaattac atcacgctca ccgctctggg ctggatcgat tcgtattacg
    14461 cactaatagt gccctgggtc gtcagcgtct ttgccatctt ccttttgagg cagcatttca
    14521 tgacgatacc gaacgaactt tacgacgccg cgatgatcga tggactgacc aaatggaggt
    14581 tcttgtggac ggtcatggtt cctctttcca aaccggccgt gattacgggg gcgctgctca
    14641 agttcgtagg gagctggaac tctttcctct gggtgttgat cgttaccaag agtcccgaaa
    14701 tcaggacact gccggtaggt cttcagaact tcagctcggc caccggtagc gactatcatc
    14761 tcttgatggc ggccgcgact ttctcggtta taccggtcgt tgttctcttc ctgttgaccc
    14821 agaagtattt cattgcggga atcgccagga gtggtttgaa atagccaggg tcaagcgcaa
    14881 gagaatcttt ccgctgggga ttttcatctt cctggttctt ttgctttcgc tccttctcat
    14941 caccaattac aacgcgatag cgaagctcaa agaggagaaa cagaggctgg agaccatgga
    15001 gaagcgatac aacgagctgg agaagcagat acagataaga gaggccgagc tggaattgtt
    15061 gaggaaattg atgcaggaga aaggcgcggg tgtctattga tgaattacat agttacaact
    15121 tcgtacaaac cgtcggggga agaagtgaca aaggccagga atctagccgg ggagatgggg
    15181 gtcaggtacg tatcgcgcaa caggttgaag gagtacgaaa aggagacagc cctggatttc
    15241 tactacgtct tcgacaagaa cggccagctc gtgatacgct cgggcgagga agtctttttc
    15301 ttccacccgg gaatgtccaa ggtcagactg aagaatatca agttgcagga ttccgattat
    15361 ctgatcaaaa gcatggaact cgcgggaact gaactggttc tcgataccac gttcggtctg
    15421 ggaaacgaag cccttctgat cgccaacttt cttcccggcg gaaaggttgt gggactcgag
    15481 gcttcagaac acatcttcag ggttgtttca cacggtctga agaattatcc ctacgaggac
    15541 gactggacga gggaggccgc cggcaggata gaactgtaca accgggacct gagagaattc
    15601 gtaaggaact gcggagaagg gtcttatgat atagtttact gtgatcccat gttcgacaga
    15661 ccacagtaca ggtcccactc gataaatccg ctgaggcctt tcgcagtgta cgacaggata
    15721 aacagggaag atgttgactg gatgatcagg gcggcccgcc ggaagttcat actcaaaagc
    15781 cggaccaggg acacgctatt ccaggagctc gggatcgaat tcgaccgttt gtggggaagc
    15841 aagaagagcg gtgttctgta cggggttatc gataagagat gataccggtt atcctcgggc
    15901 cgacggctgt gggaaagaca gccctcctgc tggagctggc catgagactc cccatagagg
    15961 tcgtatcggt tgactccagg cagatctacc gccacatgga cgtgggaacg gccaagccta
    16021 cgaaacgcga gcgagaaagt ctcagacact ggctgataga tataagggac ccggatgagg
    16081 atttcgacgt aaagaccttt agagaactgg cgctggaggc gatcgaagat atcacgtcca
    16141 gggggaagat accggtcctg gccggcggca ccggtctgta cgccgaggct ctcatgaagg
    16201 gactggccga cgttcccggc cgtaacgaga gcgttcgcga ggcccttgcc gaactcgaaa
    16261 gggccagccc cggttcttta cgaaagatgc tgaaggctct cgacagtcag gcctacgggc
    16321 gccttcacga gaacgatttg aagagatcca taaggtatct cgaggccttt ttcctgacgg
    16381 gaaagccact gagcgaagtg cagaacgaga agaccgtctc ggacagatac aaggtgatcg
    16441 tcctgaaccg ctcccggagc gagctacacg ggaggatcga attgcgggtc gaagagatgt
    16501 acaggcgggg tctgatggaa gagacgcggg aacttttagt catgggttac accgcaaata
    16561 tcaacgcgtt gaagacaata ggctacgccg aaatggtaca atgtattagg ggtttgattt
    16621 ctccacaaga agctcttgag aagataaaat gtaatacaag gagatacgcg cggcgtcaga
    16681 taatctggtt cagaaggtat tcgggtcttg aaatcgatct ttcgaatctt actgagtcct
    16741 gtgctgtatc gacactcgaa agaacaattc tcttagtttg ggggggtaat tatggctgaa
    16801 aagttcaatc tgcaggaccg ttttttaaac ctgctgaggg tcaacaaggt tgaagtgaag
    16861 atgtacctgg aaggtggttt tcagactaaa gggatggtca agtccttcga taatttcacg
    16921 gtccttctcg aggatggaca ggagcagacg cttgtttaca agcatgctat aaagatgatg
    16981 gttcctcaga aatacgtgag attgaccaat acgaccggta agaaggagga ttgaaaaaag
    17041 catagacgca ctgcttatag ctgttttctc tccgggtgaa caggaaacca ggtcggaaat
    17101 cgtcggtgaa ctcaacgaac tggcgaggac ggccaattac aatattatcg aaacagtgat
    17161 acagaatcta gattatcccg atcccaggca ttatctcggc aggggaaagg ttgattttgc
    17221 caggagaatg ctggaatctt acggtgccga aatcgtcatc acccgtcacg aactctctcc
    17281 ttctcaagct ttcaacctcg agaaacatct aggcattaaa gtaatggaca ggacccagtt
    17341 gatcctgaag atattcgcag accacgccac cactaaagaa ggtaaactcg aagtcgagct
    17401 cgccagtctt aagtatcagt tgccgaggct gaagggctac ggtagaatgc tctcgcagac
    17461 gggcggaggt atcggtacca gaggaccggg cgagaaaaag cttgaaatcg acagacggct
    17521 cgctcaggaa aagataacgc acctcaaacg ggagatcgag gaacttaaac gacagcgaga
    17581 agtctcccga aagaggaggg tcggttccgc cagcccgctg gtttctttcg tgggttacac
    17641 caatgtgggg aaatcctctc tggtttctct gctgagcggg gagaatctgc tgattcagga
    17701 taagctcttc gccaccctag ataccagggt caggagagtc aggttgattt cggggttgca
    17761 gatactcgtt tccgacacgg tggggttcat acgcgagctt ccacacgaat tgatggagag
    17821 tttcagggcg accctcgacg aagtcaggtt ctccgatctg ctggtgatag tctcggacgc
    17881 ttctga
//
LOCUS       DAOOLY010000015        17793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3280_length_17793_cov_4.650242, whole genome shotgun sequence.
ACCESSION   DAOOLY010000015 DAOOLY010000000
VERSION     DAOOLY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_17793_cov_4.650242"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLD15_01540"
     CDS             complement(<1..399)
                     /locus_tag="PLD15_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62610.1"
                     /translation="MANRKNKKLDIDSLSSSMKDSLKHKDDEVSRDLMEEREKALKAF
                     QEHFMGKTEETDPPVETRKGKKTGRRLSRGKSKVAADKGLQLFDRIRERYDLGLFSRI
                     DLTSTEKLLILFLQKIDPERTGVQISVRNLA"
     gene            complement(371..1168)
                     /locus_tag="PLD15_01545"
     CDS             complement(371..1168)
                     /locus_tag="PLD15_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPB62611.1"
                     /translation="MPVIAVANHKGGVGKTTLAYSLAYEYALGGNKTLIIDLDPSGNL
                     TIALDLDPYTTEKPNLASIVSGDRIVSCEGIVRTVNKGFAFIDIIPSSDELYRVESMN
                     NGLGSLFTLKDFLKKFGLLDRYRVIILDTPPNLGVLTNIALVCADYFLVPVSPGKYAL
                     KGLDRMFEIAQIIKKTVNPDLGFLGIVINNFRKHEQPSRALEKSVRDLYKDSYIVPPV
                     RRTVDLQKSEIMSEPIQLAFPNSDVVNDIKRLSMEVLKRGEPKKQEA"
     gene            complement(1184..1735)
                     /locus_tag="PLD15_01550"
     CDS             complement(1184..1735)
                     /locus_tag="PLD15_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB62612.1"
                     /translation="MRGQETREKIMEAAIRVIAEETIEGLSTRKVSGIAEVNLAAIHY
                     HHRSKDGMLIDLSRYILNNYVLPRLEPLIEAGKQPADLIKDLYNEVLKIYSERSDIMV
                     SLVYLWLHGMKNKRIREILINARADFYDRVERALGTSMSTRRAAKVSRRMLTYIAGKI
                     MEMSVDGETPDEREIEDMAKLLS"
     gene            complement(1830..2768)
                     /gene="arcC"
                     /locus_tag="PLD15_01555"
     CDS             complement(1830..2768)
                     /gene="arcC"
                     /locus_tag="PLD15_01555"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPB62613.1"
                     /translation="MKRIVIAIGGNALNKPNEKPTAEVMQRNLLSTTSYLADLIEEGY
                     EVVITHGNGPQVGNLLVQQDIAKEAFPPFPIDVNDAMTQGSIGYLIAQTLNNELLRRG
                     VKKNLICVLTQIVVDSNDPGFQHPSKPVGPFYSEEAARELEESKGWTVKEDAGRGYRR
                     VVPSPIPLDVLEIDAIKTLIDTGAVVIAAGGGGIPVVREPSGLVKGVEAVIDKDRASA
                     LLAKLIDADALVILTGVDFAYVNFGKENQRALTVLSLDEGHKLIKEGHFAKGSMLPKI
                     ESALDFVSSAGREAIITSLEKVDLALRGESGTRIIP"
     gene            complement(2777..3718)
                     /gene="argF"
                     /locus_tag="PLD15_01560"
     CDS             complement(2777..3718)
                     /gene="argF"
                     /locus_tag="PLD15_01560"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPB62614.1"
                     /translation="MGVNLKGKSFLTLLDFTSDEIRYLLDISKQVKVERRAGTPTNRF
                     AGKTLAIVFEKRSTRTRTAFETAFGEEGGHAVFLSKDDIHLGVKEDLKDTARVLGRMF
                     DAIAFRGFKQETVETLANWSGVPVYNGLTDLYHPTQILADFLTIEESLGSLKGRKLVF
                     VGDSRNNMANSLMIGSAKMGLHFVACGPKSLHTDTAIVDKCREIARTSGATIEVTEDI
                     DLAVKNADAIYTDVWASMGEEGKLQERIALLKPFQVNRELMERTGRSDAIFLHCLPAV
                     KGNEVTEEVFESPASRVFEEAENRKHTIKAVMIATLL"
     gene            3882..5510
                     /gene="argS"
                     /locus_tag="PLD15_01565"
     CDS             3882..5510
                     /gene="argS"
                     /locus_tag="PLD15_01565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPB62615.1"
                     /translation="MLKEKLKDSVNRVLQKMGIEEEIEFKIEIPPEGFGDLSTNAAFL
                     LSRIMKKNPREIAASFVAELQKEPEYDRVEIAGPGFINVDFSSSYLSDVLREMLSCPE
                     FWREPKSVDIQFEFASANPTGPFTVGHGRQAVFGDVLCRIFSSRGYRVQREMYINDAG
                     RQINLLGRSLWVRYNQLLGSEIDLPEDGYQGDYLIDMAREFIEKHGEKFKDTWNPQVE
                     ALFKDYALNNMLTDITGTLNDLGVRFDNIFYESSTIKDGTVENVLGKLQKSGLVYESE
                     GARWFKVSQFCEDDDKVLVRSSGSYTYFMTDIAYHTNKFERGFVKVYDIWGADHMGHI
                     PRMKAAMKALGFPDDFLNIIIHQYVNLKREGEVVKMSTRKGEFTTLDELVESVGVDAT
                     RYFFAMFDPDTHMLFDIDLAKKKSNENPVFYVQYAHARITSIFKTASEKGVVLDTDSM
                     ELLSTDEERNLVKLVSMFPEILDSIVLDYRTNRLTSYLEDLSRVFHAYYNKYIVVDPG
                     NVKTSGARLALCESVKNILKGGLELLGVSAPESM"
     gene            5522..6853
                     /gene="rlmD"
                     /locus_tag="PLD15_01570"
     CDS             5522..6853
                     /gene="rlmD"
                     /locus_tag="PLD15_01570"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPB62616.1"
                     /translation="MEPTERLKIERLVAGGYSLARRSDGKVVFLQEGYPDETVIASSV
                     KGKQDFQIMKVDRLVTPSEYRRERLCKNFPKCGGCDWQDLEYSRQLYWKGEIVKEQFR
                     RVGKMELPQLEVAPSQQRVNYRNKMEFVSYPGKEGLSLGLYARGSLIPVDCDGCVLGL
                     KDFDATRRAFQGILRKTPLRPYDRNGGSGELKHLVLRGNGSQMMAILITKGENLPGEA
                     YIAESVQKRLKNVKTLVHLINTSDKVVLRGLARVLYGDGVLAHQLSWQRFEIPTTAFY
                     QNNSGVTEAIIDHLERELKLTDEDSLLDLYAGVGTFSLTLGRKLRRVVAVESSPVSAK
                     AFRANAKINEMFAAQSVENDVGDYLKKRSDSFTTVVMDPPRSGIGRDILLVEKFRPSK
                     VAYISCDPTTLARDIALLKSSGFEIVSVKAFDMFPQTWHVETVVVLSSSKS"
     gene            6864..7769
                     /locus_tag="PLD15_01575"
     CDS             6864..7769
                     /locus_tag="PLD15_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPB62617.1"
                     /translation="MVEMKNFSDLIRLAKGREPKRVVLIGSEDREGVKALKSAVEEEI
                     AFPIFVGDENRTSQILSEEGLEGEVIPASSNEEACEKGVKFVRQGRADIVMKGLVKTS
                     TLLKAVLNKEWGLRSGNVLSHIAALDVPAIDRIVFVTDGGMVIKPDLQTKVSIIENAV
                     VFLRSIGYEKPKVALITAVEVLNGDMPETLDAAVIAKMAQRGQISNCEVDGPLGLDNA
                     LSLEAAKIKKVTGPVAGMADLLVVPEIAAGNFLGKSAVYLAGGTIAGLVVGAAAPIVI
                     VSRADSASSKLASIALASLSIGRNT"
     gene            7766..7954
                     /locus_tag="PLD15_01580"
     CDS             7766..7954
                     /locus_tag="PLD15_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPB62618.1"
                     /translation="MKVEYAGGFLSLEGEMTARELLDRLGLDFEDHLVIKDGSLVPLE
                     EKIEDSSNIIILKAVVGG"
     gene            complement(7926..8768)
                     /locus_tag="PLD15_01585"
     CDS             complement(7926..8768)
                     /locus_tag="PLD15_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB62619.1"
                     /translation="MLVDLHCHSDRSDGTDSVRSLVTKACEKGIKILSITDHDTVTAQ
                     SDARIIAESKGMTYVNGVEISCDYNETLDILGYGYDLNLDYLERTLSRLRDMRDDRND
                     QIMRKLREIGISLEERELEENFPGESLGRPHIASLLTKKGYVSSIEEAFRLYIGKGAA
                     AYVEKKKLEIGQSIELIISGGGIPVLAHPMSLKLDLDELKEMICKLKTLGLEGIEAFY
                     KEYDRDLKMQLFAVAREMDLIPTGGSDYHGEHKAHLDLGIELSDELALNFLERLNRRR
                     RPSK"
     gene            8819..9679
                     /locus_tag="PLD15_01590"
     CDS             8819..9679
                     /locus_tag="PLD15_01590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPB62620.1"
                     /translation="MILIEYYLEQGTILKRRYRINEPLGKGGFGITYLCTDLLGGERV
                     AVKEFFPRGAERNGNNVSPVSDDLREVYYDKLSSFSEEFRIISTISNDHVVKVLDFFT
                     ENNTAYYVMEFIDGLNLKEYVKNNGMPDLKTCYRILLDILDGVNAIHGRGYIHGDLKP
                     TNVMAKGDGRIKVMDFGAACLKDAFFSFLSAKVISISYTCPEKFFASSVPDNSWDIYS
                     IGGIMFFLLSGKDPVPATERVKGIPLEMSLISNRKARLIIEKAMSFSPKKRYRDSDQF
                     KKALSSRFFF"
     gene            complement(9681..10520)
                     /locus_tag="PLD15_01595"
     CDS             complement(9681..10520)
                     /locus_tag="PLD15_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB62621.1"
                     /translation="MKLEKFIPFFDRKLSDHLDGICSYSPFREIVSYTPLVGGKRLRP
                     YLVWELSDLTSFGCDSALKTGIAVELFHSGSLIHDDLPPIDNDDIRRGKPSNHSIFGE
                     ARAILAGDYLMLYPSKVISSLDLDHRYKERLVGLWSDVSLQVVEGEFADVAPSERTEE
                     LLSFIRRGKTASLFGFCFAAPFCMEDGSKFREMFELGIDFGEIFQMMDDIKDATSTLQ
                     ELGKTPGKDLSQDKLTLLSFKTVEQARALVEERFRAFCEKITEYRDLVGELEDIYRLI
                     ARR"
     gene            complement(10517..11038)
                     /locus_tag="PLD15_01600"
     CDS             complement(10517..11038)
                     /locus_tag="PLD15_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPB62622.1"
                     /translation="MLQVRLRGLALDQSNSPVVILEVEKTNKGFGIWIGPFEAEALAL
                     AVSGKDFPRPLTYDLFINTVERLGASFEMAVIGDVKDNIYYASLHLKDPSGKTMIIDA
                     RPSDCLVLAVKKSFAIFVDDRVFAESSIDLDTLQPDMIGNNQEEDMEGFKKFVENLDI
                     EELKRHFKDRDEK"
     gene            complement(11025..11801)
                     /locus_tag="PLD15_01605"
     CDS             complement(11025..11801)
                     /locus_tag="PLD15_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB62623.1"
                     /translation="MSFLKTLVSLFITLWIAIIGVLYICVYGGLVIIVGSIIGKYKGK
                     KWKKRFISREVSRFGRAAFILSGSRVTVKGKENVPEDGPMVIAANHQSAFDIPLIPGY
                     VYPGISFIAKKELSKIPGISWFIKALDGVYIDRGNKLQTAGAMRKILKILKEDGTILL
                     FPEGTRSESGELGEFKEGSLSIPFKLGVKVLPVALDGTGKLMKKNGYLITPSRINLSI
                     AEPISPEGFESEERFRKEVYNIIKKNLESSRNRGDSNVTG"
     gene            complement(11776..12990)
                     /gene="thiI"
                     /locus_tag="PLD15_01610"
     CDS             complement(11776..12990)
                     /gene="thiI"
                     /locus_tag="PLD15_01610"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPB62624.1"
                     /translation="MDKFIVVRYGEIALKQGNRKMFEKALTGNIKRQIGKSIVQRIRG
                     RIIVTVSPEKLPETEAAIKRTSGVQNYSLGSWTTYDINDIYATSIELAKIEMMKGRRT
                     FKVETRRLDKRFPIKSVELNPLVGEKILEAIPEVSVDIHNPQFVVEIEIRDEGVLISS
                     GKTHAIGGLPVGVSGRALLFLSGGIDSPVAGWLAQKRGLSIDAIHFSSPPYTGEMAFE
                     KVLSLAKRLSLFNGGRVLKFYSVHFTQAQMAIHRHVQERYSLVCQRRLMMRIADRIAD
                     KTHIKAMVTGENLGQVASQTLENMAAIAEPTGKLVLRPLITYDKIETISIARAIETFE
                     ISILPYEDCCTIFVPSSPVTKARMIDIQRVEAGLDFDEIVREALNRSQVYRIRNGDVL
                     EVEKLELSEDAC"
     gene            13062..13778
                     /locus_tag="PLD15_01615"
     CDS             13062..13778
                     /locus_tag="PLD15_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62625.1"
                     /translation="MKKLLLFLIVLGSTVFAFSSDFETLLGEFVQIRSMQDSQQMSEF
                     ITKVEKEDLNDYEILTLLADCHREYANWIEDKKTRESHYKTSRELAEKAIKLNPNYGM
                     AYYVKGASIGQLAQMAGIIQSMFLITDFDKSIDKAMELMSESPFPFIAKGMRDRDTPW
                     PYRNYDKSEDRFLKAIENDPAYINSYYELALLYEIWKKKDLAAQYYRKVVEMELQTDF
                     IVQGQESKEKAQKWLAENGY"
     gene            13780..14265
                     /locus_tag="PLD15_01620"
     CDS             13780..14265
                     /locus_tag="PLD15_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62626.1"
                     /translation="MLYRIIFSLIPLFLMPIMGHGLLRGLLVSSVMVAGVLLGRESHR
                     VSRIQNLTFVLFYALLIFGYLQNTSAPLYGDQIFLLAVAQGVSGLYGLFHRRKTLSIL
                     FACGCWTLIAVSLAQVAYAKLGTKGILLAGALMVLVALQDVRRILKPIDRNYTGRDGM
                     E"
     gene            14268..15071
                     /locus_tag="PLD15_01625"
     CDS             14268..15071
                     /locus_tag="PLD15_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPB62627.1"
                     /translation="MGKEKRIRIGVVQFNSKTDEPGENLEKAVNYIENLVEEEVDLVL
                     LPEMFNSGYGTDEGVIKSAIEMQEETIETLSALSDYNDIAIVGGVVNKTGEDLYNSSV
                     IMLPYGEPVYYNKTHLFRNEKNVFKPGNSLKSFEFAGVKFGILMCYEIGFPEISRKLS
                     REGAQVLLVPFAFGRERREIYEVATRARAIENGCFLAAASQSGRCPSMEMVGESRIVS
                     PSGEIMCDCGKGEGFCLTDLDIHQVERYRYKESGNSHGYFANMREDLYR"
     gene            15122..15197
                     /locus_tag="PLD15_01630"
     tRNA            15122..15197
                     /locus_tag="PLD15_01630"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15155..15157,aa:Lys,seq:ctt)
     gene            15318..15884
                     /gene="infC"
                     /locus_tag="PLD15_01635"
     CDS             15318..15884
                     /gene="infC"
                     /locus_tag="PLD15_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPB62628.1"
                     /translation="MAKGDSTPKNNEIVARTVRLVDVNGEQLGVVATTEALKIAREKN
                     LDLVLVSPAANPPVARIMDYGKYKYEKDKRKKEAKKKTKQTQLKEMKFRIRIDEHDFQ
                     TKTNRIQEFLEKGNKVRVVIMFRGREIVFSDKGKEILNRVSTQLQQISDVDRPPKLEG
                     RDMWMILKPKAAPQGGKENEQQSKDEDA"
     gene            15853..16056
                     /gene="rpmI"
                     /locus_tag="PLD15_01640"
     CDS             15853..16056
                     /gene="rpmI"
                     /locus_tag="PLD15_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPB62629.1"
                     /translation="MSNKVKMKTHRASAKRFKITGSGKIMRNHSGTGHNTGKKSESNR
                     REARNWTVVEGGYEKKVKKSLGI"
     gene            16080..16439
                     /gene="rplT"
                     /locus_tag="PLD15_01645"
     CDS             16080..16439
                     /gene="rplT"
                     /locus_tag="PLD15_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB62630.1"
                     /translation="MRVKGGVNSKKKKIKYLKAARGYRGALSRRYRLAKQYYIRSGVY
                     AYAGRKIKKRDFRKLWITRINAGARIAGTKYNDLIHGLKIAGVNINRKMLADLAVNDF
                     DTFKDYCELAKSTLAEK"
     gene            16482..16904
                     /locus_tag="PLD15_01650"
     CDS             16482..16904
                     /locus_tag="PLD15_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB62631.1"
                     /translation="MDYVVKRIGKMAFHARTPSGHDIHMDAKEASGGDGSAPTPMETV
                     LAALMGCTSMDIVAILNKMKVEVYKYWISANYEYAQEHPRVFTKIELVYHFAGKELPK
                     DKIEKAVSLSQERYCSVSAMLKKAVDITLKIEYEEDAT"
     gene            16901..17704
                     /locus_tag="PLD15_01655"
     CDS             16901..17704
                     /locus_tag="PLD15_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPB62632.1"
                     /translation="MNSEEMAQTMQKIIDNVKTIKNKILVMSGKGGVGKSTVAVNLAL
                     ALADEGYKTGLMDVDLHGPNVSRMVGLKKSPEIVEEQIFPPEVLPNLKVMSISSFIEE
                     DAPVVWRGPMKTTAIYQFLGDVIWGELDFLVIDSPPGTGDEPLTVLQTLTDVGALIVT
                     TPQEVAVQDVKRAINLVKTMHRKILGLVENMSYLTCPKCGERIDLFGSGGGKRLSESF
                     SIPLLASLPFDPRLVGLADNGQGIVTHMRGSQLEKAYRSMIYNLLQRVK"
BASE COUNT         4555 a         4295 c         4509 g         4434 t
ORIGIN      
        1 ggcgagattg cgtacgctga tctgcacacc ggttctttcg gggtcgattt tctgcaaaaa
       61 gagaatcagc agtttttccg tcgaggtgag atcgatcctc gaaaaaagac cgaggtcgta
      121 ccgttcacgt atcctgtcga agagctgcaa ccctttatcg gccgcaactt tcgatttccc
      181 tctggaaagt cttctgcctg tttttttgcc tttcctggtt tcaacgggag gatcggtctc
      241 ttcggttttg cccatgaagt gttcctgaaa ggccttcaag gctttttctc tttcttccat
      301 aaggtctctg gagacttcgt cgtctttgtg cttcagagag tctttcatcg aagaagagag
      361 ggaatctatg tcaagcttct tgttttttcg gttcgccacg cttcaacacc tccatcgaaa
      421 gtctctttat atcgttgacc acatcggaat tgggaaaagc cagttgaatg ggttcgctca
      481 ttatttcgga cttctggaga tccacggttc tcctgacggg cggtacgatg tagctgtctt
      541 tgtaaagatc cctcaccgac ttctcaagag ctctggaagg ttgctcatgc ttgcggaagt
      601 tgtttataac gattcccagg aaaccgaggt ccggattgac ggttttcttt attatctggg
      661 ctatttcgaa cattctatcc agtcccttga gtgcgtattt ccccggcgat acgggaacca
      721 ggaagtagtc ggcacagaca agggcgatgt tcgtaagaac gcccaggttg ggcggagtgt
      781 cgagtattat cactctataa cgatcgagca aaccgaattt cttcagaaag tctttgagag
      841 tgaagagact ccccagaccg ttgttcatcg attcaaccct gtaaagctca tcggaagagg
      901 gaattatgtc gatgaaagcg aatcccttgt tcaccgttct cactatgcct tcacaagaga
      961 ctatcctgtc tcctgacact atcgaggcca ggttgggttt ttcggtcgtg tacggatcca
     1021 ggtcgagtgc aatggtgagg ttcccggagg gatccagatc tataatcaaa gttttattac
     1081 cacctagagc gtattcgtaa gccaggctgt aagcgagagt cgtcttccca actcctcctt
     1141 tatggttggc aaccgcgatg accggcatgg tatcactccg gtatcacgag agcaactttg
     1201 ccatgtcttc tatctctcgt tcgtcaggtg tttctccgtc tacggacatt tccattatct
     1261 ttccggctat gtaggtcaac attcttctcg agaccttggc ggctctcctg gttgacatcg
     1321 atgtgcctag agccctttca accctgtcgt aaaaatcggc tctggcgttg atgagaatct
     1381 ccctgatcct cttgttcttc attccgtgaa gccacaaata cacgagcgag accattatat
     1441 cggatctctc ggagtatatc ttcaggacct cgttgtaaag gtccttaatg agatcggccg
     1501 gttgtttgcc cgcctctatt agaggctcga gtctcggtag aacgtagttg ttcagtatgt
     1561 acctcgaaag atctatcagc ataccgtctt tcgagcgatg atggtaatga atggcagcca
     1621 ggttcacttc ggcaataccc gataccttgc gagtgctcag cccttctatc gtctcttctg
     1681 ctattactct aattgccgct tccattattt tttctctcgt ttcctgacca cgcacagtcg
     1741 tcactccttt caatcgttcg tataacacaa attatatcac aatgttctgt ataaatgaag
     1801 aaccgggttt tcaacccggt tcaaaaaatt cagggtatta tccttgttcc cgactcgcct
     1861 ctcagggcaa gatcgacctt ttccagagag gttatgatcg cttctctgcc tgcgctggat
     1921 acgaaatcca gcgccgattc gatcttcggg agcatgctcc ctttcgcgaa atggccctct
     1981 tttatcaatt tatggccttc gtcgagactg agaacggtga gggccctctg gttctccttg
     2041 ccgaagttaa cgtaagcaaa atctacaccg gtgaggatta cgagagcgtc ggcatctatg
     2101 agtttagcca gaagagccga tgccctgtct ttatctatga cggcttcgac acccttcaca
     2161 agaccggaag gctctctaac gacggggatt cccccaccac cggcagcgat cacgactgca
     2221 ccagtatcga tgagagtctt gattgcatcg atttcaagca catccagcgg aatcggagag
     2281 ggtacaactc ttctgtaacc tctccccgca tcttccttca ccgtccagcc tttgctttct
     2341 tccagttctc tggctgcttc ttcgctgtag aaaggaccca cgggcttgga aggatgctga
     2401 aatccagggt cgttggaatc gacgactatc tgggtcagca cgcagatcag gtttttcttc
     2461 acgcctcgcc ggagcaactc gttgttgaga gtctgtgcga tgagatagcc gattgaaccc
     2521 tgtgtcatgg catcgttgac gtcaatcggg aacggcggaa aggcctcttt ggcaatatcc
     2581 tgctgcacca aaagattacc aacctgcgga ccgttaccgt gagttatgac cacttcgtac
     2641 ccttcttcga tcagatcggc gagataggag gtcgttgaaa gcaaattcct ctgcatgacc
     2701 tcggcggttg gcttttcgtt gggtttgtta agggcatttc cgcctatggc tattacaatc
     2761 ctcttcatac aatcccctaa agaagcgtgg cgatcattac ggccttgata gtgtgcttcc
     2821 tgttttcggc ttcttcgaat actcttgaag ccggactttc gaagacctcc tcggtcactt
     2881 cgtttccctt gacggccggg aggcagtgaa ggaagatcgc atcgctcctg cccgtccttt
     2941 ccatcaattc tcgattgacc tggaagggtt taaggagggc gattctctcc tggagcttcc
     3001 cctcttctcc catggatgcc cacacgtccg tgtatatggc gtcggcattc ttgaccgcca
     3061 gatctatatc ttcagtgact tcgatcgtgg cacccgaggt tctggcgatc tctctgcact
     3121 tgtccactat cgccgtatcc gtatgaagac tcttcggacc gcacgcaacg aagtgaagcc
     3181 ccatcttggc cgatcctatc atcagcgagt tggccatatt gtttctcgag tcgcctacaa
     3241 agaccagttt tctgcctttg agactgccca gactctcttc gatggtgaga aagtcggcca
     3301 gtatctgcgt cggatggtac agatccgtga gaccgttata aacaggaact ccagaccagt
     3361 tggccagagt ctccacagtc tcctgtttga atcccctgaa ggctatcgca tcgaacattc
     3421 taccgagaac tctggcggtg tctttgaggt cctctttcac tcctagatgt atatcatctt
     3481 tggaaaggaa aacggcatgt ccgccttcct cgccaaaagc cgtttcaaag gcagtcctgg
     3541 tcctggtaga tctcttctcg aacacgatcg ccagcgtctt cccggcgaac cggttcgtcg
     3601 gcgtaccggc ccttctctcc accttaacct gtttcgagat atcaagcaaa taccgtattt
     3661 cgtcactcgt gaagtccagc aatgtcaaga aactctttcc tttaagattt acgcccattt
     3721 cgatcctcct ttttttgtta tctctaatat aatacatata taagccgggg ctttcaattg
     3781 tctcaggctg cctgaagaaa caaggagtaa actctatcac aacaaagcgg tgttatgctt
     3841 tcgtatgctc aggtatgttt cgctgatttg gaggtggcaa agtgcttaag gagaagttga
     3901 aggattcggt caacagagtt ttgcaaaaaa tgggaatcga agaagaaatc gagttcaaga
     3961 tagaaattcc accggaggga ttcggcgacc tttcgaccaa tgcggcgttc cttttatcca
     4021 gaatcatgaa gaaaaacccc agagagatcg ccgcctcatt tgtggcagag cttcagaagg
     4081 agcctgagta cgatagggtg gagatagccg gtcccggttt cataaacgtc gatttctcat
     4141 cgagttatct gtctgacgtt ttgagagaga tgctgagttg tcccgaattc tggagagaac
     4201 cgaaaagcgt tgacatacag ttcgaattcg ccagcgccaa ccccaccggg ccgtttactg
     4261 tcggccacgg aagacaggcc gttttcggag acgttctctg caggatcttc tcttctagag
     4321 gctacagggt tcaaagagaa atgtacataa acgacgccgg aagacagata aacttgctcg
     4381 gccgttctct gtgggtcaga tacaatcaac tgctgggatc tgagatcgac cttccggaag
     4441 atggatacca gggcgattac ctgatagata tggccagaga gttcatcgaa aagcatggag
     4501 agaaattcaa agatacctgg aatcctcagg tggaagctct cttcaaggat tatgccctca
     4561 acaacatgct gaccgatatc accggtacgc tgaacgatct cggcgtgaga ttcgacaaca
     4621 tattctatga gagctcgacc ataaaggacg gcacggtcga gaacgtcctc ggaaaacttc
     4681 agaaaagcgg gctggtctat gaatcggaag gcgccagatg gttcaaagtc tcgcagttct
     4741 gcgaggacga cgacaaagtt ctggtgaggt ccagcggtag ctacacctac ttcatgaccg
     4801 atatcgcata ccatacgaac aagttcgaac gcggattcgt aaaagtatac gatatatggg
     4861 gtgcagacca tatgggccat attccaagaa tgaaagcggc gatgaaggct ctgggatttc
     4921 ccgacgattt tttgaatata ataatccacc agtacgttaa cctgaaacgt gagggcgaag
     4981 tcgtgaaaat gtcaaccaga aaaggcgaat tcacgaccct ggatgaactg gtcgaatcgg
     5041 tcggcgtgga tgctactaga tacttttttg ccatgttcga tcccgatacg cacatgcttt
     5101 tcgatataga cctcgccaaa aagaagtcta acgaaaaccc cgtcttctat gtgcagtatg
     5161 cccatgcgag gattaccagc atattcaaga cggcatctga aaagggcgtg gttttagata
     5221 ccgactccat ggaacttctc agcacggacg aggagaggaa tctggtcaag ctggtgagca
     5281 tgttccccga gattctggac tcgatagttc tcgattacag gaccaaccgg ttgacttctt
     5341 atctcgagga tctttcaagg gtcttccacg cttactacaa caagtacatc gtcgtcgatc
     5401 ccggcaatgt gaaaacctcc ggagccaggc ttgcgctgtg cgaatcggtg aaaaatatcc
     5461 tcaaaggtgg tctcgaacta ctgggagtaa gcgcaccgga gagtatgtga accgaaacct
     5521 tatggagccg acggaaagac tgaagattga aaggctagtc gcgggcgggt attcgcttgc
     5581 aagaaggagc gacggtaaag tcgtttttct tcaggaggga tatccggacg agactgtgat
     5641 agccagctcc gtgaagggaa aacaggattt tcagataatg aaggtcgatc ggctcgtcac
     5701 gccttccgag tacaggagag aaagactgtg caaaaacttt ccaaagtgtg gcggatgcga
     5761 ctggcaggac ctcgaatact cccggcaatt gtactggaag ggcgagatcg tcaaagaaca
     5821 gttcaggaga gttggaaaga tggaactccc tcaactggaa gttgcgccct ctcaacagag
     5881 agtgaattac agaaacaaaa tggagttcgt atcctacccg gggaaggaag gtctttcgct
     5941 cggtctgtac gccagaggca gccttatacc ggtcgattgc gacggctgtg ttttgggttt
     6001 gaaggacttc gacgcaacga gacgagcctt tcagggaatt ttgagaaaga ctcccctgag
     6061 gccttacgac aggaacggtg gaagcggaga actcaagcat ctcgttctcc ggggaaacgg
     6121 cagccagatg atggccatcc ttatcacgaa gggtgagaat ctcccgggcg aagcttacat
     6181 agcagagagc gttcaaaagc gtctgaaaaa tgttaagact ctagttcacc tcataaacac
     6241 cagcgataaa gtggtcctga gaggtcttgc gagagtactt tacggtgatg gggtgctggc
     6301 ccatcaacta tcctggcaga ggttcgagat ccctacaacc gctttctatc agaacaattc
     6361 aggtgtaact gaggcaatta tcgatcatct tgaaagagag ctgaagttga ccgacgaaga
     6421 ttcgcttctc gatctctacg caggggtggg gaccttctct ctcacactgg ggaggaagtt
     6481 gcggagggtt gtggctgtgg aatccagtcc cgtctcggcg aaggccttca gggcgaacgc
     6541 gaagatcaac gaaatgttcg cggcacagtc tgtcgagaac gatgtcggag attatcttaa
     6601 aaagcgatcc gattccttca ctacggtcgt tatggatccc cccaggtcgg gcatcggaag
     6661 agatatactc ctggtggaga agttcagacc ttcgaaggtg gcctatatct cgtgcgaccc
     6721 tacgaccctg gccagagaca tagcccttct gaaaagctcg gggtttgaaa ttgtttccgt
     6781 aaaggctttc gatatgtttc cgcaaacctg gcatgtggag accgtcgtcg tgctttcaag
     6841 ttctaagtca taggagggtg gctatggtag agatgaagaa cttctcggat ctgatccgtc
     6901 ttgcaaaagg cagggaaccg aagagagtgg ttctcatcgg ctcggaagat cgagaaggtg
     6961 tcaaggccct taaatcggcc gtcgaagagg aaattgcgtt tccgattttc gttggtgacg
     7021 aaaatcggac ctcgcagatc ctctccgaag aaggactaga aggagaagtg atacccgctt
     7081 ccagcaacga agaggcctgc gagaagggcg tgaagttcgt acgacagggt cgggccgata
     7141 tagtcatgaa gggactcgtc aaaacttcca cacttctgaa ggccgttctg aacaaagagt
     7201 ggggattgcg ctcgggaaac gttttgagcc atatcgccgc actcgatgtg ccggcaatcg
     7261 acaggatcgt ttttgtcacg gacgggggaa tggtcataaa gccagatctc cagactaagg
     7321 tctcgataat cgaaaacgcg gtcgtttttt tgaggtccat cggatatgag aaaccgaaag
     7381 tggcgcttat aacggctgtg gaagttttaa acggcgatat gccggagacc ctggatgcgg
     7441 cagtgatagc caagatggct caaaggggac agatatccaa ttgcgaagtg gatggcccac
     7501 tcggcctcga taacgcactg tctttagaag cggcgaagat aaaaaaggta accggtccgg
     7561 tcgccggtat ggccgatctt ctggtggttc cggaaatagc cgccggaaat tttctcggca
     7621 aatcggccgt ctatctcgcc ggaggcacta tcgccggtct ggtggtgggt gccgctgccc
     7681 ccatagttat agtctccagg gccgactcgg cttcttctaa gctcgcgtcc atagctctgg
     7741 caagcctttc tatagggaga aacacgtgaa agtggagtat gccggcggtt tcctctcgct
     7801 ggaaggggaa atgaccgccc gtgagttgct tgatcggttg ggactggatt ttgaagatca
     7861 tttagtcata aaagacggtt ctctggtacc gctcgaagag aagatcgaag actcttcaaa
     7921 cataatcatt ttgaaggccg tcgtcggcgg ttgagacgtt ccagaaaatt caacgccagc
     7981 tcatcggaca actcgattcc cagatcgaga tgggctttgt gttcgccgtg gtagtcgctg
     8041 cccccggtgg gtatcaggtc catctcgcgg gcaacggcaa agagttgcat ttttaggtct
     8101 cgatcgtact ctttataaaa tgcctctata ccctccagtc cgagggtttt gagtttgcag
     8161 atcatctctt tcagttcatc aaggtcgagt ttcaaagaca tggggtgcgc cagaacggga
     8221 attccgccac cggaaattat aagctcaata gattgaccga tctcgagttt ctttttctcg
     8281 acgtaggcag cggcgccctt gcctatgtag agacgaaagg cctcctctat cgaagagacg
     8341 tagccttttt tggtcagcag gcttgcgatg tggggccgtc cgaggctctc gccgggaaag
     8401 ttttcctcga gttctctctc ctcgagagag atgccgattt ctcgaagttt tcgcatgatc
     8461 tggtcgttgc gatcgtctct catatccctc agtcgggaaa gagtcctctc gagataatcg
     8521 aggttcagat cgtatccgta tccgagaata tccagagtct cattgtaatc gcagcttatt
     8581 tcgacaccgt tgacgtaggt catgcctttc gattccgcaa taatccgggc gtcgctctgg
     8641 gccgtaactg tgtcatggtc ggtaatggat aagatcttaa tccctttttc acaggcttta
     8701 gtgaccaggg accggacaga gtcggtgccg tctgaacggt cggaatgaca gtgtagatca
     8761 accaacatgt aaaacaccac cgttaatgag gtaaaatgct attatccaga cgcaagaggt
     8821 gatattaata gaatactacc tggaacaggg tacgattttg aagagacgtt acagaataaa
     8881 cgaacctctt ggaaagggcg gttttggaat aacctattta tgtaccgatc ttctaggcgg
     8941 ggaaagagtc gccgtgaagg aattcttccc cagaggtgcc gaaaggaacg ggaacaacgt
     9001 gagccccgtt tccgacgatt tgagggaggt atattacgac aagctctcgt ctttttccga
     9061 agagttcagg ataatctcca ccataagcaa cgaccacgtt gtgaaggttt tggatttctt
     9121 caccgagaac aacacggcgt actacgtcat ggagttcata gatggtttga atctcaaaga
     9181 gtacgtgaaa aacaacggaa tgccagacct gaaaacctgt tacaggatct tgctggacat
     9241 cctggacggt gtgaatgcca ttcacggcag gggctacata cacggcgacc ttaagcccac
     9301 aaacgtaatg gccaaaggcg acggaaggat aaaagtcatg gatttcggcg ccgcctgtct
     9361 caaggatgca ttcttctctt ttctttctgc caaggttatt tcgataagct atacatgccc
     9421 ggagaagttc ttcgcctcgt ctgtgcccga caattcgtgg gatatttatt ctattggagg
     9481 cataatgttc tttcttctga gcggcaaaga ccccgttccg gccacggaaa gggtcaaggg
     9541 aattcctctt gaaatgagtc ttatatcaaa tagaaaggcc aggttgatca ttgagaaagc
     9601 gatgtctttc tctccaaaaa aaagataccg tgactccgat caattcaaaa aggccttgag
     9661 cagtcgtttt ttcttttaga ctatcttcta gctatgagcc ggtaaatatc ttccagttcg
     9721 cctactagat cgcgatactc cgttattttt tcacagaaag cgcggaacct ctcctcgacc
     9781 agcgctctcg cctgttcgac cgtcttaaag ctgagcaggg tgagtttgtc ctgtgagaga
     9841 tccttccccg gtgtcttacc aagttcctga agggttgaag tggcatcttt tatgtcgtcc
     9901 atcatctgga agatctcccc aaaatctata ccgagttcga acatttcgcg gaatttagat
     9961 ccatcctcca tacagaaagg ggcggcgaaa cagaaaccga acagagaagc ggttttcccc
    10021 ctgcgtatga aagagagaag ctcttcggtt ctttccgatg gagcgacatc ggcgaactct
    10081 ccttcgacta cttgaagcga cacatcgctc cacaaaccta cgagcctttc tttataccgg
    10141 tgatcgagat ccagtgaaga tatgaccttt gacgggtaga gcatcagata atcaccggcc
    10201 agtatggccc gggcctcacc gaagatcgaa tggttggaag gctttccgcg cctgatatcg
    10261 tcgttgtcaa tcgggggaag atcgtcgtgt atcaaacttc cgctatgaaa gagctccaca
    10321 gcgatacccg tcttcagagc actatcacat ccaaaggatg tcaaatcgga cagctcccat
    10381 actagatacg gcctcagtct ctttccgccg accagaggag tgtacgaaac tatttcccta
    10441 aaaggcgagt aactacatat tccgtcgagg tgatcgctga gttttctatc gaagaaaggt
    10501 atgaacttct caagcttcat ttttcgtctc tgtccttgaa atgcctcttc agctcctcga
    10561 tatcgaggtt ttccacgaac tttttgaaac cctccatgtc ttcttcctgg ttatttccaa
    10621 tcatatcggg ttgaagcgtg tcgagatcta tcgaactctc ggcaaagact ctatcgtcaa
    10681 caaaaatcgc aaaactcttt ttaactgcaa ggacaaggca atcggaaggt ctcgcatcga
    10741 ttatcatggt ctttcccgac ggatccttca ggtgaagact cgcgtaatag atgttatctt
    10801 tcacatcgcc tatcactgcc atctcgaaag aggcgccgag acgttccacc gtgtttatga
    10861 aaaggtcgta agtcaaaggt ctggggaagt cctttccact aacggccaga gccagtgcct
    10921 cggcttcgaa cggacctatc catattccaa atcccttgtt cgtcttttcc acctcaagta
    10981 ttaccaccgg cgaattgctc tggtccagag ccagccctct gagtctaacc tgtaacattc
    11041 gagtcacctc gattcctgga tgattcaagg tttttcttaa ttatattata gacttctttt
    11101 ctgaaccgct cttcactttc gaatccctca ggcgagatag gttcggctat ggagaggttg
    11161 attctggagg gtgtgataag atagccgttc ttcttcatga gcttcccggt tccatccagc
    11221 gcaaccggaa gaactttgac gcccagtttg aaagggatcg agagacttcc ctctttgaac
    11281 tctccaagct ctccactttc gctcctcgtt ccctcgggga acagaagtat cgtaccgtcc
    11341 tctttcagaa tcttcagaat cttcctcatg gctccggccg tctggagttt atttccacga
    11401 tctatataca cgccgtccag agcctttatg aaccagctta tccccggtat tttcgagagt
    11461 tccttcttcg cgatgaaaga tatccccgga tagacatatc ccggtatcaa cggtatatcg
    11521 aaggcgctct gatggttggc cgctataacc atcggtccat cctcgggaac gttctctttc
    11581 cccttcactg taactctgga accggagaga atgaaggccg cccttccaaa cctcgaaacc
    11641 tcccgggaga tgaaccgctt cttccatttc tttcctttgt atttaccgat tatgctgccg
    11701 acgattatta ccagtccacc gtacacacag atatacagaa cgcctatgat cgcgatccat
    11761 aaggttatga ataaactaac aagcgtcttc agaaagctca agtttttcga cctccaggac
    11821 atctccattc ctgattctat agacttgcga ccgattcaaa gcctcccgaa cgatctcatc
    11881 gaaatcgagc ccggcctcga ctctttgaat atctatcatc cgggccttgg tgactggact
    11941 cgacggaacg aagatcgtgc agcaatcttc gtaaggcagt attgagatct caaaggtttc
    12001 gatagcgcgc gctatagaga tcgtctcaat tttatcgtaa gtgatcaacg gtctcaacac
    12061 aagtttgccg gtcggttcgg ctatcgcggc catgttttcc agagtctgac tggcgacctg
    12121 accgaggttt tcgcccgtga ccatggcctt gatgtgggtc ttgtcggcga ttctgtcggc
    12181 tattctcatc atgagccttc tctggcacac cagactgtat ctctcctgta catgcctgtg
    12241 aatggccatt tgggcttgag tgaagtgaac gctgtagaac ttcaacactc taccgccgtt
    12301 gaaaagcgag agcctttttg caagtgagag cactttttca aaagccattt cccccgtgta
    12361 aggaggactt gagaaatgga tggcatctat gctgagacct ctcttctggg ccagccagcc
    12421 ggcgaccggg ctatctatac ccccggagag aaagagcaaa gcccttccgc taacgcccac
    12481 cgggagtccc ccgattgcat gtgttttacc gctggagata agcacaccct cgtcccttat
    12541 ctctatctct acaacgaact gcgggttgtg tatatcgacg gaaacttcgg ggatggcctc
    12601 gagaatcttc tcaccgacga gagggttcaa ttcgacgctt ttgatgggaa acctcttgtc
    12661 cagccttctg gtttcaactt tgaaggtcct gcgacccttc atcatctcga tctttgcgag
    12721 ttctatcgat gtggcgtata tgtcgtttat atcgtacgta gtccaggatc caagactgta
    12781 attctgaact ccggaggttc tctttatagc cgcttcggtc tccggcagtt tttcggggga
    12841 taccgtgacg attatcctgc ccctgattct ctggacgatc gactttccga tctgcctctt
    12901 tatattgccg gtcagggcct tttcgaacat ctttctgttt ccctgcttta gggctatttc
    12961 gccgtatctg acgacgatga atttatccac tggaataact cctttcttgc atgatatata
    13021 atactcctat catacattct tttagggagg tgcgcttttt tatgaagaaa ctacttctct
    13081 ttctgatcgt tttggggagt accgttttcg ctttttccag cgatttcgaa actctgctgg
    13141 gagagtttgt acagatcaga tcgatgcaag attcgcaaca gatgtccgag ttcataacaa
    13201 aagtcgaaaa agaagatctg aacgactacg aaatcttaac gcttctggcc gattgtcaca
    13261 gggaatacgc aaactggata gaggacaaga agaccaggga atcccactac aagacatcgc
    13321 gggaactcgc ggaaaaggcg ataaagttga acccgaatta cggtatggct tactacgtga
    13381 aaggagcctc gataggacag ctggcacaga tggccgggat tattcagtcg atgtttctta
    13441 taacagattt cgataagagc atcgacaagg cgatggaatt gatgtccgaa agcccctttc
    13501 cattcatagc caaaggcatg agggatagag atacgccctg gccgtacaga aactacgaca
    13561 aatccgaaga caggtttttg aaagcaatag agaacgatcc ggcatatatt aactcttact
    13621 acgaactcgc actcctttac gagatctgga agaaaaaaga cctggccgcc cagtactaca
    13681 gaaaggtcgt cgagatggag ctccagacgg acttcatcgt tcagggccag gaatcgaagg
    13741 aaaaggcaca gaagtggttg gcggaaaacg gatattgaaa tgctttacag aataattttc
    13801 tctttgattc ccctcttctt gatgccgatc atgggtcatg gactgctgcg aggattgctg
    13861 gtctcgtccg taatggtcgc cggtgtgttg ctcggcaggg agagtcatag agtgagtcga
    13921 atccagaatc tcacgttcgt tctgttttac gcgctgctca tctttggata tttgcaaaat
    13981 acatcggcgc cattgtacgg cgatcaaatc tttctactgg cggtggcgca gggagtctcg
    14041 gggttatatg ggctctttca tcgcaggaag acgctttcta tcctgttcgc ctgtggatgc
    14101 tggacactga tagccgtatc cctggcacag gtggcatacg ccaaacttgg aacgaaaggt
    14161 atccttctgg ccggagcgct catggtcctg gttgcgcttc aggatgtgag aaggatattg
    14221 aaacctatag atagaaacta caccggacgg gatgggatgg aataatcatg ggtaaagaaa
    14281 agaggataag gatcggagta gttcagttca attctaagac agacgaacct ggcgaaaacc
    14341 tcgaaaaagc agtcaattac atcgaaaatc ttgtggaaga agaggtggat ctcgttcttc
    14401 tgccggagat gttcaacagc ggttacggga ctgacgaggg ggtcattaag agtgccatcg
    14461 agatgcagga ggagaccata gaaacgctct cggctctttc cgactacaac gacatagcca
    14521 tagtcggtgg cgtagtcaac aagaccggag aggacctcta caactcttcc gtgataatgc
    14581 ttccttacgg cgaacctgta tattacaaca agacgcacct ctttcgcaac gagaaaaacg
    14641 ttttcaagcc cggtaactca ctgaagagct tcgagttcgc gggggtgaag ttcgggatac
    14701 tcatgtgcta cgagatcggt ttcccagaga tatccagaaa gctttccaga gaaggtgcgc
    14761 aggtgttgct cgtccctttc gccttcggga gggagaggag agagatctac gaagtagcta
    14821 ctcgtgcgag ggccatagaa aacggttgtt tcctcgctgc ggcaagccag agtggcaggt
    14881 gtccttccat ggagatggtt ggagagagca gaatcgtctc gccttcgggg gaaataatgt
    14941 gcgactgcgg aaagggcgaa ggattctgtc tgaccgatct cgacatccat caggtggaga
    15001 gatacaggta caaagaatcc ggcaactctc acgggtactt cgcgaacatg cgagaggacc
    15061 tttaccgttg aagaactggt tgacaggacc ggatcgatga tatacaataa aaaacgtcag
    15121 tgggccgtta gctcagcagg cagagcgatt gattcttaat caataggttg caggttcgat
    15181 ccctgcacgg ctcaccagtt tttagattaa gcggagttta tctcccaccc ccgccttaag
    15241 gtcgaggggt cgatacctat aaggttattg acttggcggg ttattccgcc aattttcatt
    15301 tggagaggtg atgtagtatc gcgaaaggtg attcgacacc taagaacaac gaaatcgtgg
    15361 ccagaacagt tagactcgtc gatgttaacg gtgaacaact gggcgtggtc gcaacgacag
    15421 aagcgctgaa gatagccagg gagaagaacc tggatctggt tctggtttct ccggccgcca
    15481 atccgccggt ggcaaggata atggactacg ggaagtacaa gtacgaaaaa gacaagagga
    15541 aaaaggaagc caaaaagaag accaaacaga cacagctcaa agagatgaag ttcaggataa
    15601 gaatcgacga gcacgacttc cagacgaaga cgaacagaat acaggagttc cttgaaaagg
    15661 gaaacaaagt aagagtagtc atcatgttca ggggaaggga gatcgtcttt tcagataaag
    15721 ggaaggaaat cctcaacagg gtttcgaccc agcttcagca gatttccgat gtcgatagac
    15781 ctccaaaact ggagggaaga gatatgtgga tgatactcaa gccaaaggca gctcctcaag
    15841 gaggtaaaga gaatgagcaa caaagtaaag atgaagacgc atagggcgag tgccaagagg
    15901 ttcaaaataa cgggcagtgg caagatcatg agaaaccatt ccggtactgg ccataacacg
    15961 ggcaagaaga gcgaaagcaa ccgccgtgaa gccagaaact ggacggttgt cgaaggcggc
    16021 tacgaaaaga aagtcaagaa aagcctgggc atttgacccg attaagaggt ggtatttaga
    16081 tgcgagtaaa aggtggagtc aattctaaga aaaagaagat aaagtacctg aaggccgccc
    16141 gcggttacag gggagctctc agcagaaggt acaggctggc caaacagtat tacattagat
    16201 ccggagttta cgcttacgcc ggaagaaaga taaagaaaag agatttcaga aagctctgga
    16261 taaccagaat aaacgccggt gcgagaatag caggcacgaa atacaacgat ctcatccacg
    16321 gtctgaagat agccggagtc aacataaaca ggaagatgct ggccgacctg gcggtgaacg
    16381 atttcgacac tttcaaggac tactgcgaac tggccaaatc gactctggcc gaaaaatgaa
    16441 tgagaagggg cttgaagccc cttttttttg gaggatgtga aatggattac gtggtgaaac
    16501 ggatcgggaa gatggctttc cacgcaagga caccgtcggg gcacgacatt cacatggacg
    16561 cgaaagaggc ctccggtggc gacggttccg cccccacacc gatggaaacg gtactggccg
    16621 ctttgatggg ctgtacctcg atggatatcg tggctattct gaacaagatg aaggtcgagg
    16681 tttataaata ctggatatcg gccaattacg agtatgcaca ggaacacccg agggtcttca
    16741 cgaaaatcga gctggtttac cacttcgccg gtaaagaact gccaaaggat aaaatcgaaa
    16801 aggccgtaag tctttcgcag gagagatact gctcggtatc ggccatgctc aagaaggctg
    16861 tcgacatcac ccttaaaatc gaatacgagg aggacgcaac ttgaactcag aagaaatggc
    16921 ccagacaatg caaaagataa ttgacaacgt taaaaccata aagaacaaga tcctcgtcat
    16981 gtcgggaaaa ggcggagtgg gaaaatcaac ggtggcggtt aacctcgcac tcgcgctcgc
    17041 cgatgaaggt tataagaccg gattgatgga cgtcgatctg cacggtccca acgtctcgag
    17101 aatggtcggt ctgaagaaga gtccggagat cgtggaggag cagatctttc cgcccgaagt
    17161 tcttccaaac cttaaagtca tgagcatctc cagcttcata gaagaggatg cgccggtggt
    17221 ctggagaggt cccatgaaga ccaccgcgat ataccagttc ctcggagacg ttatctgggg
    17281 tgaactggac tttctggtca tagactctcc tcccggcacc ggtgatgaac cgctcaccgt
    17341 tcttcagact ctcaccgatg taggcgccct gatcgtgaca accccacagg aggttgccgt
    17401 ccaggacgtc aagagagcga tcaacctagt caagaccatg cacagaaaga ttcttggact
    17461 ggtggagaat atgtcctatc tcacctgtcc aaagtgcggc gaaagaatag accttttcgg
    17521 aagtggtggc ggtaaaaggc tctcagagag cttctccatt ccacttctgg caagtctccc
    17581 ttttgacccg agactggtgg gactggccga caacggccag ggaatagtga cacatatgag
    17641 aggctcccaa ctggagaaag cctacaggag tatgatctac aatctcttgc agagggtgaa
    17701 atagtagtgg ccaggttcaa gagcataact ctcgaatgga gcgacgacag ggtcatattt
    17761 atagaccaga gaaaacttcc gatcgaggaa gtc
//
LOCUS       DAOOLY010000016        17345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3417_length_17345_cov_5.021457, whole genome shotgun sequence.
ACCESSION   DAOOLY010000016 DAOOLY010000000
VERSION     DAOOLY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_17345_cov_5.021457"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1523)
                     /locus_tag="PLD15_01660"
     CDS             complement(30..1523)
                     /locus_tag="PLD15_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB62633.1"
                     /translation="MSSKSSSSKTFFVRGMHCQNCQLFVESKLEEVEGIKRARASLKD
                     SSVEIEFLGEVISVESINEILSGTDYSVAETKPEKPIGKAVKVLVAASLAIIVFFAVE
                     KLAIGGLINVDSSSSLVGFFLFGLVAGISSCAALVGGLVLSVSKQWYSLYSSEDSFLQ
                     KLQPHLIFHAGRLVFYSLFGFLLGWLGQSLKISLTFSSILVIAVSIVMFLIALQMLGI
                     HTVSLSIPSFLRKALAVDKSRRGRMMPFILGATTFFLPCGFTITAQALALLSGSPFHS
                     SMIMFFFALGTFFPLIAIGASSIKFFAMKSLSEVFTKIAAVLIIFFVIYNVNSQLNVL
                     GLPSLNDLTIGTGEQVSFTPDDSPTVLATAGEKEEMVMNEAGPMETGEVVDVEETVGI
                     QEVVEETLDIVQSGPDIQVIRTVASARGYTPDYYQVKAGVPVRWEIEDIGTSGCTNAI
                     ISRNLFPQRVELTRGNTSVVEFTPQLPGQYKFSCWMGHYTGIIEVVN"
     gene            complement(1766..2953)
                     /locus_tag="PLD15_01665"
     CDS             complement(1766..2953)
                     /locus_tag="PLD15_01665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB62634.1"
                     /translation="MEIRRIDSAYKERIIEIFSMSFEYFEPEESEYFFSDPSLWDYVW
                     GAFDRERLVAAYISYKYLAKIRNRIFECRYVEAVATLPEYRNGGITRKILLRDIEEAK
                     DRGVHFIMLDPFKHDFYVKLGFGLGFESLEYTFDYSLLSGDMEEDNLKLVSGKLFEKD
                     EMRELISQTRKTLWETSRYTEAIEIDACSQEIFHRKALFGAVAIDDRGLSHGMMIYGK
                     KERKMVIESFAFIDLAGLYALKRFLLNHRDQIATFEMRKMPPDFPVELLFHSRWQAGR
                     ELKIMDASSRMVRVLDPFPVVSRLMDTSPSESVVLKIKDRLLPENNVKIAIGNGKAEI
                     SEAVEDFEIEISDLATLLTGRLSPMRLWRLGKLRVGPWKNIPWGISEPPGKVRILESI
                     FPE"
     gene            3210..4289
                     /locus_tag="PLD15_01670"
     CDS             3210..4289
                     /locus_tag="PLD15_01670"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPB62635.1"
                     /translation="MDKKIKEALLVRNERIIEAVIEKSVRVCPGSVALIGIYGSFATD
                     DIHEKSDLDLFIVINDPDGYKISSCFILGDVAHDVYCTTWEQLEEMARYSNPYISRLM
                     DLKIVYCPDDKYRQRFMELRDAACKRLSSSLSESDLQNIKVHFGEAQRSYADVMMSEE
                     TERARYASARLIYYSEMIVFLMNKSYVRLGVKRRPEEIERMQRLPSGFLVSYHLLVEA
                     DSLRSIKRHATSLIRCIKDEIEEIKSKVQSKKKLDRQALEGSYEEIHSDWKNKMGLAA
                     KIGGKYLSLMTAASCQFFYDQMSEEYEGVSVDLMKHFDINDLSASAKAFDEAMEKYKL
                     LYEKNGVDVKRYATIEEFEDDYRNH"
     gene            4594..5358
                     /locus_tag="PLD15_01675"
     CDS             4594..5358
                     /locus_tag="PLD15_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPB62636.1"
                     /translation="MSGLAIAMTSFNRSRAYLLNHLVNNLGSFIFGYIYVSIWKAALG
                     DSPETQAMVTYIMVNQSILWVTMFLSRGAYIPQKIREGTIVFDLLRPYGLLFCSFFEV
                     AGHGLYSLIFRAIPIFLLGYLLLGVSPPEIGRLLPFLLTLFNGFLISFLFNYFVGLWS
                     IKFLSPTAAQGLLYMTMNLLGGAFLPAEYYPGFLRFLMPLLPFACANYYPSSVYLGTV
                     SLIRALALQCFWIVVLFLLALYMTEKLTRSIQVQGG"
     gene            5360..6136
                     /locus_tag="PLD15_01680"
     CDS             5360..6136
                     /locus_tag="PLD15_01680"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPB62637.1"
                     /translation="MRSVIALFMASLKAQYEYRLNFWLDTLINGANLFSDFLLMAFLM
                     LSFKEIGGWSLGEIAIIYSIVEMGWGIFRFFGEGLHRFEELMVTGRFDLLLIRPMNTV
                     KQLLLERIDFRRLGVVVEALAVAVFGFTASRIDFGDMWLAYISLVFFSTAMTFEINII
                     LAAIAFWTVRNSDIIVLAFYSTRTAAMYPAHIYGPVLKNVLTFIVPLASVAYFPVGYL
                     TGRITSIIALISPVLGVTALLPVTVLIWKLGLKHYASTGT"
     gene            6154..7116
                     /locus_tag="PLD15_01685"
     CDS             6154..7116
                     /locus_tag="PLD15_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB62638.1"
                     /translation="MQIVNVENLQKNYTVYQRKGLFREKRIIRALQNISFTVNRGEFL
                     GYIGPNGAGKSTTIKILTGIMTPDGGSAIVDGMVPYRERKLYVRNIGVVFGQKTQLWW
                     DLPVRDTYSLLKAIYGVPESRFKEQIGYLSGHLELGEIMDRPVRQLSLGQRMRAELAA
                     ALVHDPELLFLDEPTIGLDIVSKHRVLQFLRELHSRGKTIFLTTHDLKDIEALCQEIL
                     IINHGSLVYKGTLEGLKGMVKLPRVLKIQLREGAFSLDGRCTGFIERYAARYDSKTGT
                     IDVELSDTARPANVAREFMQVMEIEDFRITDPSIEDVVRVIYRK"
     gene            complement(7162..8505)
                     /gene="dnaA"
                     /locus_tag="PLD15_01690"
     CDS             complement(7162..8505)
                     /gene="dnaA"
                     /locus_tag="PLD15_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPB62639.1"
                     /translation="MSSQIINVLKKKVAKKTWDNWFSTFELKSVEDDRVVFSVANLFI
                     KDWLQTKYGGVINRSIHEATGKELPFEIVFKNGELSKEETVVHQQTGSLLKRKPLMIS
                     NLNPEYTFENFVVGEENRALYEVARDVTRNPGKYNPFFVHGGVGLGKTHLLQAIAQET
                     MTNFPDKKVLYITSEQFMNDMIQSIKENNIQKFRDHYRKRSDVLLIDDIQFLIGKKGV
                     QTEFFHSFNELHDAGKQLIICSDRNPEELDNFHSRLISRFQMGMLMTIQQPEAETRFH
                     IAKKLAQRESVNLPDDVAMILANNIDGNLRRLRGAIIKLIVHSSVYNTRIDISLASMI
                     LQSFASSSAVPSSQKPIDQIYDAIEHVMKISREDIKGNNRSKEIVLARQLVMYILKQH
                     FGKQVVEIARDTGKQHPTVIHSIKKIDKSVMMGRGSTKILYDDLIGLLSSRSAAV"
     gene            complement(8702..8881)
                     /locus_tag="PLD15_01695"
     CDS             complement(8702..8881)
                     /locus_tag="PLD15_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPB62640.1"
                     /translation="MKVYVDKDTCIGCGVCEGICPDVFKMTDDGKAEAIVPETEAACA
                     QDAADSCPVQAITVE"
     gene            complement(8915..9838)
                     /locus_tag="PLD15_01700"
     CDS             complement(8915..9838)
                     /locus_tag="PLD15_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPB62641.1"
                     /translation="MLRLHANDPLDKTAMKILADSGLFEITAEALDKNALLKIMPEVE
                     ILVVRSATKVTAEVIEAGKKLKLIARAGVGLDNVDAEAAEAKGVMVRNTPGANAISVA
                     ELTFGLLLGFVRQIPRGVYGIKDGKWEKKELKGNEISGKTMGIIGFGAIGRAVARIAL
                     AFGMKVVAYDPYVKSTDLEVTLVDSVDKLLPVADVVSLHIPLTPETKHIIGEKEISLM
                     KDGVIIINASRGGTIDEQALYNGLISEKVSGAALDVFEVEPPSDELRRKLIGLGNVIC
                     TPHIGASTSEGQKRVGLEMAKIIVDVCRNML"
     gene            complement(9838..10971)
                     /locus_tag="PLD15_01705"
     CDS             complement(9838..10971)
                     /locus_tag="PLD15_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPB62642.1"
                     /translation="MIKKNYLMAPGPTPVPIDVLLEGAKDTIHHRTPQFDRIFTEAVK
                     GTKEVFRTENDLFILASSGTGAMEMALTNIVNPGEKIIVCTVGKFGERWQEIAKAIGA
                     EIVLVSGEYGDFYTPEMVEKALKENPDAVAVFTTLSETSTGTVMDIEGISKVVKAAGK
                     LIAVDGISGLVAEPCETDKWGLDIVVSGSQKGFMLPPGLAFITVSKAAIEKAQNTKTT
                     SFYFNLKKYLKDPLPWTPAVNLIYQQRLAVEMLLKEGMENVWARHAVMGKVTREAIKA
                     MGLELFSKRPGNVLTSVKVPEGVPGGKIVSIMRDEYGVTIAGGQGTMKGNIFRIAHLG
                     YMSDYDVVIALTALEKVLRKLGRHVEYGVGAKVAMEIFEKEGA"
     gene            11342..12265
                     /locus_tag="PLD15_01710"
     CDS             11342..12265
                     /locus_tag="PLD15_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPB62643.1"
                     /translation="MKRLILILFLLFSLTLTLAVSTSVEKVWGSYGTKNGQFSDPQGI
                     ATDSKGNVYVADTLNHRVQVFDSNGKHKSSFGKFGEKNGEFRFPHDIVVDSKGNIYVA
                     DSQNGRVQKFNDKYKFVKAWGSKGAGEGQFDGAMFMAIDSSDRIYVGDAFNQRVQIFD
                     SEGLLLRTVGGKVTMMESMDDGKLATVSGLAVDATGNLYICDDFNRRIQKFDPQGNFL
                     LKWTRGVSGNFELPGQIAIDKSGNVFFLEKRQNRVYMLDPSGNYIYEWGSEGTGNGQF
                     KSPFGIEIDKSGFLYVLELTGNRVQKFKINY"
     gene            12365..13351
                     /locus_tag="PLD15_01715"
     CDS             12365..13351
                     /locus_tag="PLD15_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62644.1"
                     /translation="MKKVVISILFVSLIFVASFSQRLLPKDTAKFIDIVTGGTFNTFP
                     DRFIGEAFEEYFESPRWRYYKDIEEDVVEFTGTFTDEGGQPATVTMRFTVKEVDETFV
                     LAFWAINGEPQDNNLQYAFLEKIFIPDEKERVISVVKEGYFYDFPDKIIGEYFDNFFD
                     DTSWDFFLSGDGLNVVEFIGHHYPEGNLEEVVMQFTVDLNERTFDIAYVGVNDETKDD
                     AYIDTMLGNILGSPLTLVKGSYFDIYYNTMTLGEAFDWFFADPYWSYFISTEDLVIVE
                     FSGSFDLGGVPAQVYMQFEVYEDGYYDLYYWEIDESYESLNAFYLLLETIYY"
     gene            complement(13416..15209)
                     /locus_tag="PLD15_01720"
     CDS             complement(13416..15209)
                     /locus_tag="PLD15_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB62645.1"
                     /translation="MSLKRYSIEELIKASTIASFAVSKDSSKLMYSSDDRGHYDVYQV
                     DLMNGDKSRLTEYDENAMVKHVFDDESFLFMMDKGGNELHHLYLNRGGEVVDLTPVEG
                     TKSGFHQFSGGYVYYFSNRLDKTRFDLYRLNQTSLESELIFENKEVMDLGPVSDDGRY
                     VGLSRQDTANNSEMFIYDRSTGETKHLSRHTGEVKFLPVFFSKDSMELYYLTDERGEF
                     MALRAMKLDSLHSREVLALDWDISDVERSHDGRYAALVVNRDSFFELQILDLARDAVT
                     NVRFSPGGMIYQQAFDRDSKKVFVLADSAVTPTEIHVLSIADGQVKTLTNSASAEVES
                     ADLVTPTILRFSSYDGTVVPGVFYLPADASPEKKAPALIWVHGGPGGQSFPRYNPELQ
                     FLVNHGYAVYAVNNRGSSGYGKSFFKAADHKHGEADLDDCVAAAQYLATMEEIDSERI
                     GIIGGSYGGYMVLAALAFRPKVFKIGVDIFGVSNWVRTLKEIPSWWRAIKDLLYRKIG
                     DPFGEEEYLRSISPLFHASKIERPLLVLQGVNDPRVLKVESDEIVQKAREKGVPTGYV
                     VFDDEGHGFTRKVNRITAAKAMLEFLDRYLV"
     gene            complement(15284..16201)
                     /locus_tag="PLD15_01725"
     CDS             complement(15284..16201)
                     /locus_tag="PLD15_01725"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPB62646.1"
                     /translation="MICDHSVELGAIGSLASPVMISSGTFGVAKPYMELLRGQPVGAV
                     VTKTITLNPKAGNPQPRIYEGPCYLINSIGLENQGVDRFVDFYNDEYGEIGFPVIVSI
                     SGNSVDEFVTIARKLNSIDGLLGLELNLSCPNVDGEGLAFGSVPERIGEVVKACGSCS
                     RFPLIAKLSPFQAIDDSFALAAARAGAAAISLINTVPAMVLDIHRFRPATGALSGGMS
                     GPAVKPIALKMIYEISRSVDIPVIGMGGIASGEDAIEFLMAGTRAISIGTMNMVDPSL
                     PGQIMGKIAGFMERHDIENLPEISRRFHL"
     gene            complement(16191..16934)
                     /locus_tag="PLD15_01730"
     CDS             complement(16191..16934)
                     /locus_tag="PLD15_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPB62647.1"
                     /translation="MISNKSVSIDGNLITFSSEKIAHSALPGQFVEVSCSDGGMLLRR
                     PFSVYDTTAESLSLFVKSVGRGTRWLGGLREGEKVNIIGPLGKGFSIDEGSRSLLIGG
                     GCGVASLRLLSKAIVEAGGECDAIFGFRKREEIPETIVGDFSRTARRLTVTVDSGDYP
                     VRGNVMDRLAEIEFDRYGRYYCCGPTAMLRALVPLLSLKTTEVSLEAKMACGLGVCYG
                     CTINTDRGGKRVCYDGPVFTMKEVEWNDL"
     gene            complement(16955..>17345)
                     /locus_tag="PLD15_01735"
     CDS             complement(16955..>17345)
                     /locus_tag="PLD15_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB62648.1"
                     /translation="LISIIASDHAPHSAEDKRLDKPARGFSGVELLFSLCYSGLVRKA
                     IIDLERLVEFLSLSPSLLLGIEPVCFEAGKRADIVLFDPEEEWRVEESAMHSKGKNTP
                     FMGGRLKGRVKYTIASGEVIYPFGELR"
BASE COUNT         4072 a         4534 c         4388 g         4351 t
ORIGIN      
        1 agagtcctgg cctcttgcaa gagcgtttgt cagttcacaa cttctatgat ccctgtgtag
       61 tggcccatcc agcaactgaa tttatactgg ccgggaagct gcggggtgaa ttctacaacg
      121 ctggtgttgc ccctggtgag ttctacacgt tggggaaaga ggtttcgtga gatgatagcg
      181 ttggtgcaac cgctggtgcc aatatcttcg atctcccacc tgacgggaac gccggctttc
      241 acctggtaat aatcaggcgt ataacctctc gcgctggcta cagttcttat aacctgtata
      301 tccggtccgc tctggacaat atccagagtc tcttccacaa cttcctgaat tccaaccgtc
      361 tcttcgacat ctacgacctc tcccgtttcc attggcccgg cttcattcat aaccatctct
      421 tctttttcac cggccgttgc cagaacggtc ggcgaatcat cgggagtaaa tgaaacctgc
      481 tcgccggttc cgatggtaag atcgttcagg cttggcagtc ccagaacgtt tagctggctg
      541 ttcacgttgt agatgacgaa aaatattatc agaaccgccg ctattttcgt gaagacttcc
      601 gataagctct tcatggcgaa gaacttgatt gatgaagccc ctatggcgat gagcgggaag
      661 aaggttccaa gcgcgaaaaa aaacattatc atcgagctat ggaaaggaga gcccgagagt
      721 agcgccagag cctgggccgt tatcgtgaag ccgcagggaa ggaaaaaggt tgtagcgccc
      781 agtatgaaag gcatcattcg tccacgtctg gatttatcta ctgccagcgc tttacgtaaa
      841 aaggaaggga tcgatagact caccgtgtgg attcccagca tttgtagtgc aattaaaaac
      901 atcactatcg agaccgctat cacgaggatc gaagaaaagg tcagcgagat tttcagactc
      961 tgtccaagcc agcccagcaa gaatccgaaa aggctgtaaa agaccagacg tccggcatga
     1021 aagatcagat ggggttgtag cttttgaaga aaagaatcct cagaactgta aagcgaatac
     1081 cactgtttgg aaacggaaag aaccagacct cccacgagag ccgcgcagct cgatatccct
     1141 gctaccagtc cgaaaaggaa gaagcctacc agcgacgagc ttgaatcgac gtttatcaag
     1201 ccgcctatgg cgagtttctc cacggcgaaa aagactataa tggccagaga agcggcaacg
     1261 agtaccttga cggcttttcc gataggcttt tccggcttcg tctcagcgac actgtagtcc
     1321 gttccgctca agatctcgtt gatactttcg accgagataa cctctcccag gaattcaatc
     1381 tcgaccgagg aatctttcag ggaggctctg gcccttttta tgccttcaac ttcttccagt
     1441 ttactttcga caaagagctg gcagttctgg caatgcatgc cgcgtacgaa aaacgttttt
     1501 gatgaagagg atttcgagct caactgagtc acctcgcaga tgaagagtga ataaaaccaa
     1561 caataattgt ctgattgtag catatagcag acagtttttg tctactcaaa agtgtgacag
     1621 atacgttttg ctatggttca gaaaatcgat ggacctcacg aagaaaggcc gaggaatgtc
     1681 ggctggcagc ttttcttata tcgattgacg tcgagtcggc ttgataggga aaggtgacta
     1741 acctgtcaga tagcgttgtg cgtaatcact cggggaatat ggattccaga attcgaactt
     1801 tcccgggtgg ttcggaaatt ccccacggga tgttcttcca ggggccgacg cgcagctttc
     1861 cgagtctcca cagtctcatt ggcgaaagtc ttccggtgag aagagtggcg agatctgaga
     1921 tctctatctc gaaatcctcc accgcttcac ttatctctgc cttcccgttt ccgatcgcga
     1981 ttttcacgtt attttccggc agcaatcgat cctttatctt caaaacgaca ctctctgagg
     2041 gagaagtatc catcagtctc gaaacgaccg gaaacggatc gaggactctt accatacgcg
     2101 atgaggcgtc cataattttt agctctctcc cggcctgcca tctcgaatgg aagaggagtt
     2161 ccaccggaaa atcgggcggc atcttccgca tttcaaaagt tgcgatctga tctctgtgat
     2221 tcagcagaaa cctcttcagc gcgtagaggc cggcgagatc gatgaaggcg aagctctcga
     2281 tcaccatctt cctttctttt ttgccgtata tcatcattcc gtgggacagc cctctgtcat
     2341 caatggccac ggcgccaaag agcgccttcc tgtgaaatat ctcctgactg catgcgtcga
     2401 tctcgatagc ttcggtatag cgagaggtct cccagagagt tttacgggtt tgcgaaatta
     2461 gttctctcat ctcgtctttc tcaaaaagtt tcccggaaac gagcttgagg ttgtcctctt
     2521 ccatatcacc cgacaggagg gaatagtcga aggtgtactc gaggctctcg aaacccagcc
     2581 cgaacccgag cttcacgtag aaatcatgct tgaaggggtc gagcatgatg aagtgaacac
     2641 ctctatcttt ggcttcctcg atatctctca gcaggatttt cctggttatc ccaccgttgc
     2701 gatactcggg aagggtggcg acggcctcga catacctgca ttcgaagatt ctgttccgga
     2761 tcttcgcgag atatttgtag cttatgtacg ctgcgacgag tctctcccgg tcgaatgcgc
     2821 cccaaacgta gtcccagaga gaagggtcgg aaaagaaata ctcgctctct tccggttcga
     2881 aatactcgaa cgacatcgag aagatctcga ttattcgctc cttgtatgca ctgtcgatcc
     2941 ttcttatttc cattcaacgc cccctttcaa accgtaccaa caatgatacc agatcaggcc
     3001 gtcgggcggt tcccgcctga tgatctcctg gttgacaaaa acagacagta cagcataaag
     3061 acagactgga ccgaatcagc gtctcgcact ctttctgcgg gagagtttga tagaattgct
     3121 ctgtgcaaac tcgccgatcc accggaggcg tgtattagcc gtcgagtaac ggacggtctt
     3181 ttccatctat ctatgaaatg gaggcgctta tggataagaa gatcaaagag gcccttttgg
     3241 ttcgaaacga gaggatcatc gaggccgtga tcgagaaatc agtgcgtgtt tgccccggtt
     3301 cggtcgcact gataggtatc tacggctcat ttgccacgga cgatattcac gaaaagtccg
     3361 atcttgatct gttcatagtg ataaacgatc ccgacggcta caagatcagt tcatgcttca
     3421 ttctgggcga tgtcgcgcac gacgtctatt gcacgacatg ggagcaactc gaagaaatgg
     3481 cccgatactc gaatccgtac atctccaggt tgatggatct caagatcgtt tactgtccag
     3541 atgacaagta ccgtcaaaga ttcatggagc tccgcgacgc tgcctgtaaa cgtctatcat
     3601 cctctttgag cgagtccgat ctgcagaaca taaaagtcca cttcggggag gctcaaagat
     3661 cgtacgcgga cgttatgatg agcgaagaaa ctgaaagagc caggtatgca tctgccagat
     3721 tgatctacta ttccgaaatg atcgtatttc tcatgaacaa atcgtatgtg aggctcgggg
     3781 taaaacggag gcccgaagag atcgaaagga tgcagagatt accgtccggt tttctggtta
     3841 gctaccatct actggttgaa gccgactcgc tgcgatctat caagaggcac gcaacatcgc
     3901 tgattcgatg tattaaggat gaaattgaag agatcaaaag caaggtgcag agtaagaaaa
     3961 aactcgaccg gcaggctctg gaggggtctt acgaggaaat acactccgac tggaagaaca
     4021 agatggggct ggccgcgaaa atcggcggca aatatctttc gcttatgacg gcagccagct
     4081 gccagttttt ctacgatcaa atgagcgaag aatacgaagg cgtttccgtg gatctgatga
     4141 agcactttga catcaacgat ctttcagcat cggcgaaggc atttgacgaa gcgatggaaa
     4201 agtacaaatt gctttacgag aaaaacggcg tcgatgtgaa acggtatgcg actatcgagg
     4261 aattcgagga cgactaccgg aatcattagg taaaatccta tcaaggcgag gatatatagc
     4321 ggggtctctc ttcctctctc tttctctctc tcttctctct ctccctctct ctcttctctc
     4381 ctcttcccgc gcttacgcca acagccatta ttggaaagtg aactggtcgt cggaagcggg
     4441 atgactgttg caatctctcc ggtagtgtag taatattcta tttactccta ttgattgcag
     4501 gaatactctc ctgcagtttt tttatgcctt ttttcagttg atccactctc gcaacttcaa
     4561 cccggtaaat caaccctgaa ggaggtgttg tgtttgtccg gtctggccat cgccatgact
     4621 tctttcaaca ggagccgggc gtatcttttg aaccatctgg tcaacaacct cggcagtttc
     4681 atcttcggct acatctacgt gagtatatgg aaggcagcgc tgggagattc tccggaaacg
     4741 caggcgatgg tgacctatat catggtgaat caatcgattc tctgggtcac gatgtttctc
     4801 tcgcgcggag cctacattcc gcagaagata agggaaggga cgatcgtttt cgatcttttg
     4861 agaccttacg gattgctttt ctgcagcttc ttcgaggtcg ccggtcacgg tctatacagt
     4921 ttgatcttca gggcgatccc gatattcctt ctgggctatc ttctgctcgg cgtatcgccc
     4981 ccggagatag gaaggttgct tcccttcttg ctgactctgt tcaacggttt tttgatatcg
     5041 ttcctcttca actatttcgt cggattgtgg tcgatcaaat ttctcagccc aacggctgcg
     5101 cagggacttc tgtacatgac catgaacctg ctgggaggcg ccttccttcc ggccgaatac
     5161 tatccgggat ttctccgttt cctgatgccg ctcctgccgt tcgcctgcgc caactactat
     5221 ccttcgagcg tttatctggg cacggtgagc ctgatcaggg ccctggcttt gcagtgtttc
     5281 tggatagtcg tgctttttct tctggcgctg tacatgacgg aaaaactcac gagatcgatc
     5341 caggtccagg ggggctgaga tgaggagcgt tatcgcactt ttcatggcca gtctcaaggc
     5401 gcagtacgag tacaggttga acttctggct ggatacgctg atcaacggag ccaacctttt
     5461 cagcgacttt ctgctgatgg ccttcttgat gttgagtttc aaggagatcg gcggctggag
     5521 tcttggagag atcgcgatca tatattcgat cgtcgaaatg ggatggggga tcttcaggtt
     5581 tttcggcgag ggacttcaca ggttcgaaga gctcatggtg accggaaggt tcgaccttct
     5641 cctcatcaga cccatgaaca cggtgaagca actgctgctc gagaggatag atttcaggcg
     5701 gttgggagtc gtggtagagg ccctggccgt tgccgttttc ggttttacgg ccagtcgcat
     5761 cgattttgga gatatgtggc tcgcttatat ctctctggtc ttcttttcga ccgccatgac
     5821 tttcgagata aacataatac tggccgcaat agccttctgg actgtcagga actccgacat
     5881 aatcgttctg gcgttctatt cgacgagaac ggcggcgatg tacccggcac acatctacgg
     5941 gccggtcctg aaaaatgtcc tgaccttcat cgtgccactg gcgagtgtgg cctactttcc
     6001 tgtgggctat ctcacaggaa ggataacgag tatcatcgcc cttatctcgc cggttttagg
     6061 ggtgaccgcc ctgctgccgg taacggtctt gatatggaag ctcggtctca aacactacgc
     6121 aagcacggga acctgataac cgaaaggagt cttatgcaaa tagtcaatgt cgagaatctt
     6181 caaaagaatt acacagttta ccagagaaag ggtttgttta gagagaagcg cataataagg
     6241 gcgctgcaga acatctcctt caccgtgaac cgcggggagt ttcttggcta cataggcccc
     6301 aacggcgccg gcaaatctac gaccatcaaa atactgacgg gaatcatgac ccccgacggc
     6361 ggctcggcca tcgtcgacgg gatggttcct taccgcgaga ggaagcttta tgtcagaaac
     6421 ataggggtcg tcttcggcca gaagacgcag ctctggtggg acctccctgt aagagacacc
     6481 tactcgctcc taaaggcgat ctacggagtt cccgagtcgc ggttcaaaga acagatcggt
     6541 tatctttcgg gccacctcga gctgggtgag ataatggaca ggccggtcag acagctgagt
     6601 ctgggccaga ggatgagggc cgaactggcg gccgctctcg ttcacgaccc cgaacttctg
     6661 ttcctggatg agccgacgat cggactggac atcgtttcaa agcacagagt cctccagttc
     6721 ctcagggagc ttcattcgag gggcaagacc atcttcctga cgacgcacga tctgaaggac
     6781 atagaggcgc tctgccagga aattctgatc atcaaccacg gcagcctggt ctataaggga
     6841 actcttgaag gactgaaagg catggtgaag cttcccaggg ttctgaaaat acagttgagg
     6901 gaaggcgcct tttctctgga cggtagatgc accggcttca tcgagagata cgcggccagg
     6961 tacgactcga agacgggaac tattgacgtt gaactgtcgg acacagccag gccggcgaac
     7021 gtggcgcgcg agttcatgca ggtgatggag atagaagatt tcagaataac cgatccctcg
     7081 atagaagatg tggtcagggt aatttacaga aagtgatcga gcgtttttgc cgggaatagg
     7141 aagaacttgc gcgaggttcg gtcaaacggc cgccgacctg gatgaaagca ggcctatcag
     7201 atcgtcgtag agtatcttgg tgctgcccct tcccatcata acgctcttgt ctatcttctt
     7261 gatcgagtgt atgacggtag ggtgttgctt tccggtatcg cgtgctatct ccacgacctg
     7321 cttgccgaaa tgctgtttga gtatgtacat cacgagctgc ctggcgagaa ctatctcttt
     7381 gctccggtta tttcccttta tatcctctct ggatatcttc attacgtgtt cgatcgcgtc
     7441 gtagatctgg tcgatcggct tctggcttga aggaacggcc gacgaactgg cgaaggattg
     7501 caagatcatg ctggccagcg atatatcgat cctggtgttg taaacgctgc tgtgaactat
     7561 gagcttgatt atggcccccc tgagtcttct gaggttcccg tcgatgttgt ttgccagtat
     7621 catcgccacg tcatctggga ggttcaccga ctcgcgctga gccagctttt tcgcgatgtg
     7681 gaacctcgtt tctgcctccg gctgctgtat ggtcatcaac atgcccatct gaaacctgga
     7741 tattagcctg ctgtgaaagt tgtccagctc ctcgggattt ctgtcggaac atattatgag
     7801 ttgtttgccg gcgtcgtgaa gctcgttgaa ggaatggaag aattcggtct ggacgccctt
     7861 ctttcctatg aggaactgta tgtcgtctat gagaagaacg tccgatctct tccggtagtg
     7921 atccctgaat ttctggatgt tgttctcttt tatagactgg atcatgtcgt tcatgaactg
     7981 ttcgctggtg atatagagaa ccttcttgtc ggggaagttc gtcatggtct cctgggcgat
     8041 ggcctggagg aggtgggtct ttcccagtcc gacgcctccg tgaacgaaga agggattgta
     8101 cttaccggga ttacgtgtga cgtcgcgcgc cacttcgtag agcgccctgt tttcttcgcc
     8161 gaccacgaag ttttcgaatg tgtattcggg attgaggttg gaaatcatca gaggttttct
     8221 cttcagaaga gagccagttt gttgatgtac taccgtctct tctttcgaaa gctctccgtt
     8281 cttgaagact atctcgaaag gcagctcctt tccggtggct tcgtgaatcg acctgtttat
     8341 gactccaccg tacttggtct gaagccagtc ttttataaag agattcgcga cggaaaagac
     8401 aaccctgtca tcctctaccg atttcaattc aaaagtggag aaccagttgt cccaggtctt
     8461 tttggcaacc ttctttttga ggacgtttat tatttggcta gacacaacta cctccacgaa
     8521 acagaaactg taaccccgaa gcaaaccgca caatcgaaaa aagaacccca aatgatctcg
     8581 ctgaagtaat tactactata ccagaatcta accacagaaa aaatagacaa attttgtgtt
     8641 ccactataga aaggggcaga caaaaagtcc gcccctagat gtgacagtta agaatatgcg
     8701 actattctac tgttattgct tgaactggac aggaatcggc tgcgtcctgc gcacaggcag
     8761 cctcggtttc gggaacgatt gcctcggcct tgccgtcgtc ggtcatcttg aaaacgtcgg
     8821 ggcaaatccc ttcacaaaca ccgcaaccta tgcatgtatc tttgtcaacg taaaccttca
     8881 cttcttacac ctccgcctag aatatcgatg taaattataa catgttacgg caaacatcta
     8941 cgatgatttt cgccatttcg aggccgactc tcttctgtcc ttccgaagtg ctggctccga
     9001 tatgcggcgt acatatcacg ttgccgagac cgatcagctt tctgcgcaat tcgtccgacg
     9061 gcggttcaac ctcgaaaaca tccagcgcgg caccggagac cttctcggat atcaaaccgt
     9121 tgtaaagagc ctgttcgtct atcgtgccgc cacggcttgc atttattatg ataacgccgt
     9181 ccttcatcag agagatctct ttttcgccaa ttatatgttt cgtttccgga gtcagaggga
     9241 tgtgcaggga cactacatcg gccactggaa ggagtttatc gacggaatcg accagagtga
     9301 cttcgagatc ggtggacttg acataaggat cgtaggcgac gaccttcatg ccgaaagcca
     9361 gcgctattct ggccacagcc cttccgatgg cgccgaaccc gattattccc atagtcttgc
     9421 cggagatttc atttcccttc agttctttct tctcccactt tccatccttt atgccgtaaa
     9481 cacccctggg gatctggcga acgaatccaa gaagcagtcc aaaggtcagc tcggccaccg
     9541 atatggcgtt ggcgccgggc gtgttcctca ccatgactcc cttagcctcg gccgcttcgg
     9601 catctacgtt gtccagaccg acgccggccc tggctattag cttgagcttc ttgccggcct
     9661 ctatcacttc ggccgtcact ttcgtggcgc ttctcacgac cagaatttca acctcgggca
     9721 ttatcttcag cagggcgttc ttatccagcg cttcggccgt gatctcgaag aggccgctgt
     9781 cggccagaat cttcatcgcc gttttgtcca gaggatcgtt ggcgtgcagt ctaagcatca
     9841 tgcgccctcc ttctcgaaga tctccatcgc aactttggcc ccgactccgt attcaacgtg
     9901 gcgaccgagt tttctcagaa ccttctcgag agccgtcaac gcgattacca catcgtaatc
     9961 ggacatgtaa ccgagatggg cgattctgaa gatgttgccc ttcatggtcc cctggccgcc
    10021 ggcgatggtg acgccatact cgtcgcgcat tatggagacg atcttgccgc ccggtacgcc
    10081 ctcgggtacc ttgacagaag tgagaacgtt gcccggtctc ttcgagaaaa gctcaagccc
    10141 catggccttg atggcctctc tcgtgacctt tcccataacg gcgtggcgcg cccagacgtt
    10201 ttccattcct tccttgagga gcatttcgac tgccagtctc tgctggtaaa tgaggttgac
    10261 ggccggtgtc catggaagcg ggtctttgag gtatttcttc agattgaaat agaagcttgt
    10321 ggtcttcgtg ttctgtgcct tttcgatcgc cgctttgctt acggtgatga aggccaggcc
    10381 cgggggaagc atgaagcctt tctgcgaacc ggaaactacg atatccagtc cccacttgtc
    10441 ggtttcgcag ggctcggcga ccagaccgct tatgccatcg accgcaatca gctttccggc
    10501 cgctttgacc accttgctga taccttcgat atccataacc gtgcctgtcg aagtttcgct
    10561 caaagtcgtg aaaaccgcaa ccgcgtcggg attttccttc agcgccttct caaccatttc
    10621 cggagtgtag aagtctccgt actcaccgct gacaagcact atctctgcgc cgatggcctt
    10681 ggctatctcc tgccatcttt caccgaactt tccaacggta cagacgataa tcttttctcc
    10741 tggattgacg atgttggtta atgccatttc cattgcgccc gtaccggagg atgcgagtat
    10801 gaagagatcg ttctcggttc tgaagacctc tttcgtaccc ttgacggcct ctgtgaaaat
    10861 cctgtcgaat tgcggagttc tgtgatggat cgtatctttt gccccctcga gaagaacatc
    10921 gatagggaca ggggtcggcc cgggagccat caggtaattt ttcttaatca tctttgccaa
    10981 ttcaggcacc tccattcgat aataaagtac attctgtatc agatggaaca tcgtgaaaaa
    11041 ataaattacg tctgagacca gacgggaaga gctgcttttt ttattttcac actactagtg
    11101 tagaataatt agccaacctt aacaacaccc ggaattcacc aacaaagata atgatcgctg
    11161 gttgaggcaa aacatctacg ggaaactctg cgtaactctt tccgataact tcatttgaac
    11221 ccgtgagaga ctgcgggcat tgttcgggca atctgccgtg tccaagagag ttttcaagtc
    11281 tcaaaaaacg acaggcgatg ttatcatcta acgatcaaca tcacaaaggg gagtggttgc
    11341 attgaaacgc ctgattctta tcctttttct gttgttctca ctcacgctga cgctggcggt
    11401 atcaacctcg gtggagaaag tctgggggag ttacggaaca aagaacggtc agttcagcga
    11461 tccacagggt atagcgaccg acagcaaggg aaatgtgtat gtggccgata cgctgaacca
    11521 cagggtacag gtcttcgatt cgaatgggaa gcacaaatcc tctttcggca aattcggaga
    11581 gaagaacgga gagttcagat tcccgcacga tatcgtagtc gattctaaag gaaacattta
    11641 cgtggccgat tctcagaacg ggagagttca aaagttcaac gacaaataca agttcgtgaa
    11701 ggcatggggc agcaaggggg ccggcgaagg acagttcgac ggcgcgatgt tcatggcgat
    11761 cgattcctcc gacagaatat acgtgggaga cgccttcaac cagagggtac agatcttcga
    11821 cagcgaggga ctccttctga gaaccgtcgg cggaaaggtt accatgatgg aatccatgga
    11881 cgacggtaag ctcgcgacgg tttccggact cgccgtcgat gcaaccggca acctctatat
    11941 ctgcgacgat ttcaacagga ggatacagaa gttcgacccg cagggcaact tcctcctcaa
    12001 gtggacgaga ggtgtcagtg gaaacttcga gttgcccggc cagatagcca tagataaatc
    12061 gggaaatgtc ttctttctgg agaagcgaca gaacagggtc tatatgctcg atccatcggg
    12121 caactacatc tacgagtggg gaagcgaagg cacgggaaac ggccagttca agagcccctt
    12181 cggaatcgag atcgacaaga gcggattcct ttacgttcta gaactcaccg ggaacagggt
    12241 tcagaagttc aagataaact attaagcggt cggatgactg tcattaatag ggtttgtctg
    12301 tagggccatt tcagtgtatt atttaatgat agccagaaaa aacggctgaa aggaggttgt
    12361 tcgtatgaaa aaggttgtaa tttcgattct cttcgtttcc ctcatctttg tggcctcttt
    12421 ctctcaaaga ttgctgccga aagacaccgc caagttcata gacatagtta cgggaggtac
    12481 tttcaacact ttccccgaca gatttatcgg cgaagctttc gaggagtatt tcgagagccc
    12541 gaggtggcgt tattacaaag acattgaaga agatgtggtg gagttcaccg gcacctttac
    12601 agacgaggga ggccagccgg cgaccgtcac gatgcgtttc acagtgaagg aagtcgatga
    12661 gaccttcgtt ctcgcgttct gggcaatcaa cggtgaaccg caggacaaca atctgcagta
    12721 cgcattcctt gagaagatat tcattcccga cgagaaggaa agggttatat ccgtggttaa
    12781 agaaggctat ttctacgatt tccccgacaa gatcatagga gagtacttcg ataatttctt
    12841 cgacgacacc agctgggatt tcttcctgtc cggcgacggc ctgaacgtgg tcgaattcat
    12901 cgggcaccat tacccggagg ggaatctgga agaggtcgtg atgcaattca cggtcgatct
    12961 caacgaaaga accttcgaca tcgcctacgt gggagtgaac gacgagacca aggacgatgc
    13021 ctacatagat acaatgctgg gcaatatact cggctctccg cttacactgg taaagggcag
    13081 ttacttcgat atctactaca acacgatgac cctgggagag gccttcgact ggttctttgc
    13141 cgatccctac tggagttact tcatttccac agaagatctg gtcatagtgg aattctcggg
    13201 ctccttcgat ctcggcggcg taccggcgca ggtctatatg cagttcgaag tttacgaaga
    13261 cggctattac gatctttact actgggagat cgatgaatcg tacgagagtt tgaacgcttt
    13321 ctatcttctg ctagagacga tctactattg ataattctat attgaaagag gcccctgcgg
    13381 gggcctcttt tcttctactt gaaatgagcc gttcttcaaa caaggtacct gtccaggaac
    13441 tccagcatcg ctttggccgc ggtgatacgg ttgaccttgc gggtgaagcc gtgtccctcg
    13501 tcgtcgaaga cgacataccc ggtcggtaca cctttctcgc gagccttttg aactatttcg
    13561 tcggactcca ccttcaacac gcgcggatcg ttcacgccct gcaagaccag cagaggcctt
    13621 tcgatcttcg aggcgtggaa gagaggcgat atgcttctca aatactcttc ctcgccgaag
    13681 gggtccccga tcttcctgta cagcagatcc tttatggccc gccaccagga gggaatctcc
    13741 ttgagggtcc tcacccagtt ggaaactccg aagatatcga caccgatttt gaagacctta
    13801 ggcctgaagg ccagcgcagc cagaaccatg taccctccgt agctgccgcc gatgatacct
    13861 atcctctcgg agtcgatctc ctccatcgtg gccagatatt gagcagcagc cacgcaatcg
    13921 tcgagatcgg cctcgccgtg cttatggtct gcggccttga agaagctttt tccgtaaccg
    13981 gagctccccc ggttgttcac ggcgtaaacg gcgtaaccgt gattcacgag aaactgaagt
    14041 tccggattgt agcgcgggaa agactgacct cccggtccgc cgtgaaccca gatgagcgcc
    14101 ggtgcctttt tttctggtga tgcgtcggcc ggcagataaa acacgccggg cacgaccgtt
    14161 ccgtcgtatg aggagaagcg cagaatcgtc ggggtcacaa gatcggcgct ttcaacttcc
    14221 gcactggccg agttcgtgag cgttttcact tgcccgtcgg caatcgagag aacgtggatt
    14281 tccgtcggcg taacggccga atccgcgagc acgaagactt ttttcgagtc cctgtcgaaa
    14341 gcctgctggt agatcatgcc acccggcgag aacctgacgt tggtcaccgc atccctggct
    14401 agatcgagta tctgcaattc gaaaaaggag tccctgttca ccaccagggc cgcgtaccgg
    14461 ccgtcatgcg atctttctac atcggaaata tcccagtcca gggccagaac ctccctgctg
    14521 tgaagcgaat cgagtttcat ggccctcaga gccatgaatt cgcctctctc atcggtgagg
    14581 tagtacagtt ccatggagtc cttagagaag aaaaccggga ggaatttgac ctcgccggtg
    14641 tgccgcgata agtgcttcgt ctcgccggtc gatctgtcgt aaatgaacat ctcagagttg
    14701 ttggccgtgt cctgtctgga aagaccgacg taccttccgt cgtcggaaac cggtcccaga
    14761 tccatgacct ctttgttttc gaagataagt tcgctttcaa ggctggtctg atttagcctg
    14821 tacagatcga aacgcgtttt atccaatctg ttcgagaagt aatacacgta tccgccgctg
    14881 aactggtgga aacccgattt cgtcccttcg acgggcgtga gatctacgac ctctccgccc
    14941 cggttcagat aaaggtggtg cagttcgttg ccgcccttgt ccatcatgaa caaaaagctt
    15001 tcgtcatcga aaacgtgctt taccatcgca ttttcatcgt attcggtcaa ccttgactta
    15061 tcaccgttca taagatcgac ctgatagaca tcgtaatgac cgcgatcgtc cgaggagtac
    15121 atcaatttgc tcgagtcctt cgaaacggcg aaggaagcga tcgtcgaagc ctttatcagt
    15181 tcttcgatcg agtatctctt caatgacata ggcgtatcct cctgttttta gatagaaaga
    15241 ttatacactg aagccggcct cgatcgacct ttatgcgcgg ttattacaaa tggaatcgtc
    15301 tgcttatttc cgggagattt tctatgtcgt ggcgttccat aaaaccggcg attttcccca
    15361 ttatctgccc gggaagagag ggatccacca tgttcatcgt tcctatcgag atagccctcg
    15421 tccctgccat caggaactct atggcatcct cgccggaagc gatgccaccc attccgatca
    15481 caggtatatc gacgctcctg gaaatctcgt agatcatctt cagcgcgatc ggttttacgg
    15541 ccggtcccga catgccgccc gacagcgcgc ctgtggctgg cctgaatctg tgtatatcca
    15601 gcaccatcgc gggaacggtg ttgatgagag agatggcagc cgcaccggcc cgggcggccg
    15661 cgagcgcgaa ggagtcgtct atggcctgaa agggactcaa cttggcgatg agggggaagc
    15721 gactgcacga gccgcacgct ttgacgactt cccctatcct ttcagggacg cttccgaagg
    15781 ccaatccctc gccatctacg ttgggacagg agaggttcag ctccagtcca agcagtccat
    15841 cgatcgagtt caatttcctg gctatcgtca cgaattcatc gacggaattg ccgcttatgc
    15901 ttacgatgac cggaaaacct atctcgccgt attcatcatt atagaaatcg acgaagcgat
    15961 cgactccctg gttctcgagc ccgatcgagt tgattagata gcaagggccc tcgtagatcc
    16021 tcggttgagg attcccggcc ttggggttga gtgttatcgt tttggtgacg acggccccga
    16081 caggctgtcc gcgcagcaac tccatgtacg gtttcgccac tccgaaggtc ccggaggaaa
    16141 tcatgacggg actcgccaga ctgcctatgg cccctagctc cacgctgtga tcacaaatca
    16201 ttccactcaa cctctttcat ggtgaagacg ggaccgtcgt aacagaccct ttttccacct
    16261 ctgtccgtat ttatagtaca tccataacat acacccagcc cgcaggccat tttggcctcc
    16321 agcgagactt ccgtcgtctt caaagaaaga agaggaacga gagccctgag catggccgtg
    16381 ggtccgcagc agtagtacct cccgtaacga tcgaattcta tctcggccag tctgtccatc
    16441 acgttgcccc tgaccggata atctccggaa tctacggtaa ccgtcagacg ccgggccgtc
    16501 cgcgagaaat ctccgacgat agtttcgggg atctcctctc tttttctgaa gccgaagatc
    16561 gcgtcgcatt caccaccggc ttccacgatc gccttcgaca gcagcctcaa cgacgctacg
    16621 ccgcatcctc cgccgatcaa gagcgaccgg cttccttcat cgatcgaaaa gcccttcccc
    16681 agaggtccta tgatgttgac cttctcgccc tctctcagcc cgcccagcca gcgtgttccc
    16741 cttccgacgg atttaacgaa gagagagaga ctctccgcag ttgtatcgta tacggagaaa
    16801 ggccgtctca ggagcatccc cccgtccgag cacgagactt ccacaaactg cccgggaagg
    16861 gccgaatgcg ctatcttctc cgaggagaag gttataaggt ttccgtcgat gctgacgctt
    16921 ttatttgaaa tcacggcgca attttccgat actgtcatcg taactccccg aatggataga
    16981 tcacttctcc ggaagcgatc gtgtacttga ctcttccctt caacctgccg cccatgaagg
    17041 gcgtgttctt gcctttcgag tgcatcgcgg actcctcgac gcgccactct tcctcaggat
    17101 cgaagaggac tatatcggcc cgttttccgg cttcgaaaca gaccggctcg attcccagaa
    17161 gcagggacgg cgagagcgag agaaactcca ccagtctctc cagatcgatt atcgcttttc
    17221 tcaccaggcc gctgtagcag agcgagaaga gcagctcgac tcccgaaaag cccctggccg
    17281 gtttatcgag cctcttgtct tcggcgctgt ggggggcgtg atcggaggcg attatactta
    17341 tgagg
//
LOCUS       DAOOLY010000017        17096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3490_length_17096_cov_4.796373, whole genome shotgun sequence.
ACCESSION   DAOOLY010000017 DAOOLY010000000
VERSION     DAOOLY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_17096_cov_4.796373"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLD15_01740"
     CDS             <1..405
                     /locus_tag="PLD15_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPB62649.1"
                     /translation="TAVREKLAVVGKTLEEIGGGEIKSLHVFNKIDRCTDERLVELRC
                     IFPDSVMVSALKVENIDGLVLAIQRALDVEKKRWTLRLKPEALTSFMKFRDSLEIVSQ
                     EYGDDVIEIQYLSSDEIHERMISSICEEGKCL"
     gene            405..1418
                     /locus_tag="PLD15_01745"
     CDS             405..1418
                     /locus_tag="PLD15_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62650.1"
                     /translation="MRKLLAVLVVTLAAGILLSAILPFPLKRSPEITGYFGEFRGNSR
                     NINYPEHFHMGLDYSTGSIVGLDLLSPDDSYVNVIYINHPIYGMGIAITLPNVTNVLT
                     NDKGINVIYAHLNEIGDTSSLTGRKLNDLYHQLSSEFGDQYVEVVFDSRELPFKRNEV
                     VAKSGNSGNVAPHLHLEVRDKRMETIINPGLYFDTGQPSSAIEILDLRVGGKVYSFSS
                     GKPTVEMTSSTPIDLHTKVQLRHPVSPRSIELYVENSLIYQIDFMFFDEDEAASVYEI
                     YSSPSNESDYWFNLNSRKSLSVLPVNIWDDIDWTNPRDAKVVVRDHWGNEASKEFRIV
                     MRR"
     gene            1424..1729
                     /locus_tag="PLD15_01750"
     CDS             1424..1729
                     /locus_tag="PLD15_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62651.1"
                     /translation="MFFKKKERDVSKNPFYDSGSTVVVHFQCGKCGEKFRSHLRKYYD
                     ISVNYGKGKGAYRLDKEFIGGNCQNKIQIYADFNRAFKPLSFDIIGGKFLSKEEYEE"
     gene            1754..2935
                     /gene="metK"
                     /locus_tag="PLD15_01755"
     CDS             1754..2935
                     /gene="metK"
                     /locus_tag="PLD15_01755"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPB62652.1"
                     /translation="MKTWLFTSESVTEGHPDKMADQISDAILDAMLTQDENSRVAVET
                     LLATGVAVVAGEVSTKAYVDIPRVVRDTILEIGYNRAKFGFDGETCAVLTSIDEQSPD
                     IALGVNKSLEAKRDDSDRYDTIGAGDQGMMFGYATDETPEMMPLPIVMAHRLALRLAH
                     VRKEGIVKGLRPDGKTQVTVKYSDGRPVGVTTVVVATQHDPDLTREEIESLVVENVIS
                     PVIDDELLLEGVEIFVNPTGRFVRGGPAADTGLTGRKIIVDTYGGWIPHGGGAFSGKD
                     PTKVDRSAHYMARYAAKNIVAAGLAEKVTLQLAYAIGVARPVSFMIDTHGTEKVDLEK
                     LRKAVLKTFDFRPGAIIDKLDLRRPIYRKTAAYGHFGRTDIDLPWEKVDMIEQLKKAL
                     G"
     gene            2979..3257
                     /gene="rpsT"
                     /locus_tag="PLD15_01760"
     CDS             2979..3257
                     /gene="rpsT"
                     /locus_tag="PLD15_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPB62653.1"
                     /translation="MPNNASAKKRVRQTAKKTMINKAVKTKFRNASKKLYKAIEQGED
                     ASSVASMLSEVYSTLDKAAKVGTIHKNTASRKKARLTATVKSYVQAKQ"
     gene            complement(3312..4448)
                     /gene="ugpC"
                     /locus_tag="PLD15_01765"
     CDS             complement(3312..4448)
                     /gene="ugpC"
                     /locus_tag="PLD15_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPB62654.1"
                     /translation="MAEVILEKVGKTYPNGFKAVIDANLKVDDKEFVVLLGPSGCGKT
                     TTLRMIAGLEEITEGTIKIGGKVVNDVEPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKTPKPEIEQRVKEAARILGIEQLLDRKPKQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRAELKRLHQNLQATIIYVTHDQVEAMTMADKIVIMKDGIIQQIGD
                     PYSVYFEPRNKFVAGFIGTPAMNFISAKLVSEGGKTWVVKEDMKVLVPTDKAAILKDL
                     VGKNITFGIRPEDIYDKMYAVAPKEEFTVKGSVDVVEPLGSETLIHANIHGDDIVAKV
                     DPKSRAAAGQVIDLVFDMTMLHVFDPETEENVLAGTHENAQPKM"
     gene            complement(4464..4730)
                     /locus_tag="PLD15_01770"
     CDS             complement(4464..4730)
                     /locus_tag="PLD15_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPB62655.1"
                     /translation="MVHLSRLHELSFYSLAEAKAHFSRVVDDCEKADIIITKNGVPKA
                     VLMDYGKYVLLNKFLERVHDLYLMDAGDEAMNVEIRDLIVEVDD"
     gene            4794..5720
                     /locus_tag="PLD15_01775"
     CDS             4794..5720
                     /locus_tag="PLD15_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPB62656.1"
                     /translation="MQKKFFQKQPMMRKVIYSLIPILIFAIGNFGWVVLIKTFLSTFM
                     AVFIEWLFERKKGKPVSEAVIVTGLLVGLILPPAVPFWIVIVSSSFGVVFAKMAFGGF
                     AKNLYNPAMVGRAFVYVSFPAAVQQSWTPAVSTWGQGFGRWIASDIVTMATPVNVIRG
                     GGDMSMWKMFFGNVSGTSGETAKWLIIAAAVYLIITKTASWKIILSTLVSAFVMSGII
                     FLFDPTRLNPLAWIFGGSILFGAVFMATDPISAPKKERSKWIYGGLIGALSVVIGTFS
                     LFGAGFMFALLIANTFASLIDYLNTPGKVKKS"
     gene            5717..6337
                     /locus_tag="PLD15_01780"
     CDS             5717..6337
                     /locus_tag="PLD15_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB62657.1"
                     /translation="MKDKLYSVFFAFALTLVFTLVLASINALASGTIEVNEKLDFQKS
                     LLYVFGFVQKDDKLSSKEIQELYGEKIVELHGEVDVYRAQVEGESVYAFTVQSPGLWG
                     TITGLIAVNTDGTRLVGIDFVQHSETPGLGGRIDEKAFQQQFRDERISAGPSGRIAVV
                     SGQNTSSSEDSKVDAITGATRTSEAIQKLIDKAMNEVFPKVLKVVN"
     gene            6339..6938
                     /locus_tag="PLD15_01785"
     CDS             6339..6938
                     /locus_tag="PLD15_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPB62658.1"
                     /translation="MAESKKETFMANIWKNNPVIVQILGICSALAVTNNMTNTLIMGI
                     GLTFATGFSSLTISALRKYIPKNVRMIVQVLIIATYVIILDIVLKAYLPSISKALGPY
                     VGLIITNCIIMGRAEAYAQSHGPVLSFIDGVSAGVGYSLILLLIAFIRELLGFGSIFG
                     LRVLPESFTPWVIMITAPSAFFIIGTIIWIARTPLVKKK"
     gene            6954..7544
                     /locus_tag="PLD15_01790"
     CDS             6954..7544
                     /locus_tag="PLD15_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPB62659.1"
                     /translation="MGVPLNPFVIMVAAALTSNMVLSNFLGMCSFIAVSKDIKTANGL
                     GMAVTFVLTLTSVLNYLVYYYILVPLELVYLRYIVFIIVIAAFVQFLEMLIDRFSPSL
                     YMNLGIFLPLITVNCAILGGVLFMIIRDYNLMQSLFFGFGSGAGWWLAIVALSTMRMR
                     LREKDIPPQLVGPGLTMIIIGIMALAFLGFSMVPVQ"
     gene            7549..8655
                     /locus_tag="PLD15_01795"
     CDS             7549..8655
                     /locus_tag="PLD15_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HPB62660.1"
                     /translation="MAQLQILITALVLGGISAVLAAIISVADAVLNNYGDVKLTVNGS
                     KSYTVKGGSTLLTTLAGEKIFVPSACGGKGSCGLCKVKVLSDIGPILPTELPYLTTKE
                     KSENVRLSCQVKVKGDISIEIPEGLFNIREYASTVSTIKDLTHDIKEVFFSFDDDINF
                     RAGQYVQLIVPPYGEIKESTMRAYSMSSQPSIKNGVELLIRLVPNGIVTTYVHKYLKE
                     KDEVRLLGPFGDFFLRNTDADIIFIAGGSGMAPIKSIILDMKEKGIKRNAYYFFGARS
                     KKDLFYLDLMREIEKEVPNFHFIPALSSPLPEDNWQGEFGLITDVVDRYLTEQGEIER
                     EGYLCGSPGMINACITVLGKHKIPENRIYFDKFG"
     gene            8675..9133
                     /locus_tag="PLD15_01800"
     CDS             8675..9133
                     /locus_tag="PLD15_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62661.1"
                     /translation="MKQTPDLGKVQENMKAGQITGPGFLGSDERNLIDIIREDEQKIS
                     ELGLTNEIIADKLEMLMRQGEKGFGSPIVVDNRFVVIVDESRGYVPCPFRHGNLSKKV
                     NVNVRSMALKKEIDYTPISIHLIREHGFYQGKGSLYRLDPEEVAKFLELI"
     gene            9227..9817
                     /locus_tag="PLD15_01805"
     CDS             9227..9817
                     /locus_tag="PLD15_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62662.1"
                     /translation="MKRRLPNRAIFTFVFIIALISFVAYFASKYSVKVVVPTDVSLDE
                     LLFNAEHFSLLGEAEYPPDQGLANGIAYEDEKGIIHQVTYPDYDDSVRGYLLDLDSGS
                     NVIKVFVWNSGSVTQAKETWERLYMIEGSVMTRERGRIKKAGYFYARIIRFGGKDETL
                     LWQKDRWVVLVKSPAVLEKKTEEIILTGLFDPKVRS"
     gene            complement(9801..10253)
                     /locus_tag="PLD15_01810"
     CDS             complement(9801..10253)
                     /locus_tag="PLD15_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPB62663.1"
                     /translation="MNRIIVSACLVGINCTYRGDNNLSLELLRLVDRFILFPVCPEQL
                     GGLPTPRSKAEITVSNDWTTRRIVIDQFGHDVTAQFHRGAEEVLKIARFCSTGIAVLK
                     SKSPSCGCRGIYDGTFSGVTVDGMGITAELLLKNGIEVYPESEINFLL"
     gene            complement(10250..11098)
                     /locus_tag="PLD15_01815"
     CDS             complement(10250..11098)
                     /locus_tag="PLD15_01815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPB62664.1"
                     /translation="MNRRVILVLLFVSLLLVTRSFAYFLVTYVVRSGDSIHTISRELG
                     VSMSTIIDFNNLSDPNNIKAGDRLKLPQPDGLIYEVQSGDTIDGIAKLFFAPVKELLS
                     ANNLNSSSLISPGQRIFVPMTLINMYQYVPHTTPYRWPVYGVISSSYGWRTHPVNGGA
                     SFHGGLDIAAPQGTPIFAASKGVVIAAGVNGGYGNAVDIQHDNGYVTRYGHMSKISVY
                     VGQRVDAGSLIGRVGSTGVSTGPHVHFEVKDPKMNTLDPLSMLPTRDLMYVIRREDDG
                     TAAGGK"
     gene            complement(11095..11532)
                     /locus_tag="PLD15_01820"
     CDS             complement(11095..11532)
                     /locus_tag="PLD15_01820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPB62665.1"
                     /translation="MRKFLKSLHHAANGLGYLLKERNFRIQLVFGGAVLTIGALIRLE
                     RQDFLWLLFCTFMVVILEAVNTVVEKIADILHPYYDERVKVLKDVAAAIVLMGSAMSV
                     LIGLSILIKEIFDLHFAVGILFGIIIVVFFFSFGLFRVGGGKE"
     gene            complement(11529..12140)
                     /locus_tag="PLD15_01825"
     CDS             complement(11529..12140)
                     /locus_tag="PLD15_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB62666.1"
                     /translation="MKKAFPQPHTVRISGIGGQGNVLMGIILAEALLDQGSWVVQTQS
                     FGAQVRGGLSYCDVLFNTEPIDYPRAHSFEVIYSMHQMAVNAHVPLLHMNGILIIDST
                     QVKSIPKEGARMTRKIISKPVTELTEGKFGTSLPANMVGLGLIARAGNFVTTQALKVA
                     MSKHIKAKYVDMNSQAIDFGYSLIERSYNLRSERIDSVGRGFE"
     gene            complement(12137..12973)
                     /locus_tag="PLD15_01830"
     CDS             complement(12137..12973)
                     /locus_tag="PLD15_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPB62667.1"
                     /translation="MAVSRLTKYLRQDRMPHVWCPGCGNGVIMKAFVDAVDRESLDPD
                     RIAVVSGIGCSSRVTGYLNFNTMHTLHGRAVAFATGVKLARPEFNVIVMGGDGDMMAI
                     GGNHFIHACRRNMDITTIVFNNNIYGMTGGQYSPTTPTEKIASTSPYGNVENSFDIVK
                     VAMAAGATYVARSTVIHYAQAVQFIRNGLKHKGMAVIEIMTNCHTYYGRYNSLSKPQD
                     MLNYFKTNTIQLAKAKTMSPEEIGDKIVAGEFVNVDTETYIDRYEKMREKVLAAEGGE
                     RE"
     gene            complement(12987..13490)
                     /locus_tag="PLD15_01835"
     CDS             complement(12987..13490)
                     /locus_tag="PLD15_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPB62668.1"
                     /translation="MSGEELEKFLNGFKTHKSKTPSESWDSYFMKLAYQVRERSSCHH
                     RKVGALIVRENRILATGYNQPPSGFPHCDETECIRDALNIPSGQNQELCYAAHAEQNA
                     LSQAARFGIATNGSTIYVTHRPCSICARLIINAGIKRVVFGEGYPDPLTEFMFDRTGV
                     IMINYSM"
     gene            complement(13513..14667)
                     /locus_tag="PLD15_01840"
     CDS             complement(13513..14667)
                     /locus_tag="PLD15_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HPB62669.1"
                     /translation="MYLHLPFCARRCAYCDFCTLQLDETSLKKYHAALKSEIELFSDG
                     AAVDTLYFGGGSPSLYPLDLLQELLDTLRSRFRFELREATLEANPWELTFERLERWKK
                     LGVNRLSVGLQSSDGEILKTVNRPVPPDLFERLEMAVDLFDNVNFDFILGLPGENRKN
                     VENNLKLIERLKPAHISYYMFDSDHETDLMASVKNDIIELPDKDTLEKLHDFIVLSLK
                     ELGLTRYEISSWSKGRMSLHNLKYWKSGDYKGFGVSAGSHEGTTRSVNCDSLDQYIDS
                     IVAGKRPLAYIRENTPLEELFETLFMGLRLIDGLTLSAEIFPADLLERSVERIASVLG
                     EFLVVNGYRLSLNDYGLDMSRLVFERLVGIMEEMESLLPSRATVEKQKNT"
     gene            complement(14679..15500)
                     /locus_tag="PLD15_01845"
     CDS             complement(14679..15500)
                     /locus_tag="PLD15_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62670.1"
                     /translation="MKKTVCFLIVILVCSLSLSQTQIQFTNRGWSLSTDIKAYENFHL
                     TLALASGMPYVGITYKDHWEEFSSGFLKMKIRDGDFRGSILASHEGLYMGARYVSNTN
                     QIPVFSHSLLELELSSLGDRRYLVRNWFRLPLGQINGGLSTFIYRVDDFAFNDIGLYL
                     YAKEFERSLRLRNAETNVVVTVDLKTNDSLGEMGYGFGVGMNYMSFDLGAAFSGNFIV
                     PAGDLKLVLSPMIMVSLRGIDAQLLISKLGSDDSVYAGISITNFKLNGVFMRLIF"
     gene            complement(15497..16942)
                     /gene="gatB"
                     /locus_tag="PLD15_01850"
     CDS             complement(15497..16942)
                     /gene="gatB"
                     /locus_tag="PLD15_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB62671.1"
                     /translation="MYRTVIGLEIHAQLQTKTKAFCSCPADVFELEPNTAICPVCTGQ
                     PGALPVLNENVVDYAIMAGLAFNCTLNERSVFDRKNYFYPDLPKGYQITQYFFPIAEN
                     GYLDIEVGGVGKRVRIRRIHIEEDAGKMIHQGTDSITGSTGSHLDLNRCGVPLIEIVT
                     EPDLETPAQAKVFMELLRDTLRALGVCSGDMEKGALRCDANISMRDDASGLSSNRVEV
                     KNINSFKFVEKALESEQERISAVLDAGGNVAKETRSWNFATKETFSMRSKEEENDYRY
                     FPEPDLPVLMITQDRIGRLRESLPELPWQKLRRFIEQYGLPEYDASVLSSSGEVACFY
                     EEVARVTGKPKESSNWIMGEVMRIMNEGDLSIESLGLKPEYFKELFEMIDSGRISNSI
                     AKEILPEVIERKRSPASIVKEKGLEQINDDGVIETAIREAMESNPKAVQQFREGKEGV
                     LGYFVGAVMKATRGKANPARVNEMARRLLKD"
BASE COUNT         4117 a         4416 c         4374 g         4189 t
ORIGIN      
        1 acggccgtca gggaaaagct cgcggtcgtc ggaaagacgc tcgaagagat cggcggggga
       61 gagataaaga gcctccacgt cttcaacaag atagatagat gcacagatga gagactcgtt
      121 gaactgcggt gcatcttccc ggactctgta atggtcagcg cgctcaaggt agagaacatc
      181 gacgggctcg tcctggcgat ccagagggcg ctcgatgtgg agaaaaaaag atggactctc
      241 aggctgaaac cggaagctct gaccagtttc atgaaattca gggattccct cgaaattgtt
      301 tcgcaagagt acggagacga tgtgattgag atacagtact tatcctccga tgagatacat
      361 gaaaggatga tttcttctat ttgtgaggag ggaaaatgct tatgatgaga aaactgttgg
      421 cggttctggt agttactctg gcggccggta tcctgctctc ggcgatactg ccctttccgc
      481 tcaagagaag tcccgagatc acagggtact tcggagagtt cagaggtaat tcgagaaaca
      541 tcaattatcc ggaacacttc cacatgggtc tggattactc gaccggaagc atagtaggcc
      601 tcgatctgct ctccccggac gattcgtacg tgaacgttat ctacatcaac cacccgatat
      661 acggaatggg catcgcgatc accctgccga acgtcacgaa cgttctcacc aacgacaagg
      721 gtataaacgt catatacgcc catctcaacg agatcggcga cacctcttcg ctgacgggca
      781 ggaagttgaa cgatctttac catcagctct cttccgagtt cggcgaccag tacgtcgaag
      841 tcgtcttcga ttcgagagaa cttcccttca aacgcaacga agtggtggcc aaatccggca
      901 acagcggaaa cgttgcccca catctgcacc tggaagtaag ggataaaaga atggaaacga
      961 taatcaaccc cggcctctat ttcgatacgg gccagccttc ctcggcaatc gagatactcg
     1021 acttgagggt aggtggaaag gtttacagtt tttcttcggg caaaccgacc gtggaaatga
     1081 cctcctcgac cccgatagat cttcacacca aggtgcagct cagacaccct gtcagtccca
     1141 gaagcatcga gctttacgtc gagaacagcc tgatctacca gatagatttc atgtttttcg
     1201 acgaggacga ggcggccagt gtctatgaga tctactcctc accctcgaac gaatcggatt
     1261 actggttcaa cctcaactcc agaaaatcac tcagcgtgtt gcccgtgaat atctgggacg
     1321 atatagactg gaccaacccc agagacgcga aggtcgtggt gcgcgaccac tggggaaacg
     1381 aggcttctaa agagttcagg atagttatga ggaggtagtt gaaatgtttt tcaaaaagaa
     1441 agagagagat gtttcgaaga accccttcta cgacagcggc agcacggtgg tggtgcattt
     1501 tcagtgtgga aaatgcggag agaaattcag gagtcattta cgaaaatact atgacataag
     1561 tgtgaattac ggaaagggaa agggcgctta cagactggac aaagagttca ttgggggcaa
     1621 ttgccagaac aagatccaga tttacgccga ctttaacaga gccttcaagc ctctctcctt
     1681 tgatataatt ggtggtaaat tcctttctaa agaggagtac gaagaataga ttcagctata
     1741 aggaggcaat tagatgaaaa cttggttgtt caccagtgaa agcgtcacgg aaggtcatcc
     1801 cgacaaaatg gccgatcaga tatccgatgc gatactcgac gcgatgctga cgcaggacga
     1861 aaattccagg gtggccgtgg agacgcttct ggcgacggga gtagccgtgg tggccgggga
     1921 agtcagcacc aaagcgtacg tggatattcc cagggtagta agggatacga ttttggagat
     1981 cggctacaac agggcgaagt tcggtttcga cggcgagacc tgcgccgttc ttaccagcat
     2041 agacgagcag tcgcccgata tagctcttgg agtgaataaa tcgctcgaag ccaaaagaga
     2101 cgattcagat agatacgata ccataggagc cggcgatcag gggatgatgt tcggttacgc
     2161 caccgatgaa actcccgaga tgatgcctct tccgatcgta atggcccaca ggctcgccct
     2221 caggctggcc catgtgagaa aagagggaat agtgaagggc ttaaggcccg acggaaagac
     2281 gcaggtaacc gtgaagtact ccgacggccg gcctgtaggc gtcaccaccg ttgtggtcgc
     2341 tacccagcac gaccccgatc tgaccagaga agagatcgaa agtctggttg tggagaatgt
     2401 tatttcgcct gtgatagacg acgagctgct tctggaagga gtggagatct tcgtcaatcc
     2461 caccggtcgc ttcgtcaggg gcggtccggc ggccgacacc ggtctcaccg gaagaaagat
     2521 catagtcgat acatacggcg gatggatccc gcacggcgga ggtgccttca gcggaaagga
     2581 cccgaccaag gtcgatagat cggcccacta catggccagg tacgccgcca agaacattgt
     2641 agccgccgga ctggccgaga aggtcactct gcaactggct tacgccatag gtgttgcccg
     2701 gccggtttcc ttcatgatcg acacccacgg gacggagaaa gtcgatctcg agaaattgag
     2761 aaaggccgtt ctgaagacct tcgatttccg gcccggcgcc atcatagaca aactcgatct
     2821 cagaaggccg atatacagaa agacggccgc ttacggccac tttggaagaa ccgatataga
     2881 tctaccctgg gaaaaagtcg atatgatcga gcaattgaag aaagccttgg gttaatgata
     2941 aaataatctg taaatcccga aaggaggaga agtgctaagt gcctaacaat gcttcagcaa
     3001 agaagagagt gagacagacg gccaagaaga ccatgatcaa caaggctgtc aaaaccaagt
     3061 tcagaaacgc ctctaagaag ctttacaaag ccatcgagca gggcgaggat gcttcctcgg
     3121 tcgcctcgat gttgagtgaa gtatattcca ccctcgataa ggctgcgaaa gtgggaacga
     3181 tccacaaaaa tacagcttca cgcaagaagg ccagactgac cgcaacggtc aagtcttacg
     3241 tgcaagccaa gcagtaatcc gggaagaaaa aaaggtggcg cgagccacct ttttttatgt
     3301 gtgaaactcc tttacatttt cggttgtgcg ttttcgtgcg ttccggccag tacgttctcc
     3361 tcggtctcgg gatcgaagac gtggagcatg gtcatgtcga atacgagatc gatcacctgt
     3421 ccggccgcgg ccctgctctt gggatcgacc ttggccacaa tgtcgtcccc atggatattg
     3481 gcgtgtatca gcgtttcgct tcccagtggc tcgacgacgt cgacgcttcc cttgactgtg
     3541 aattcttcct tcggggcaac cgcgtacatc ttgtcgtaga tatcctcggg cctgatgccg
     3601 aaggttatgt tcttgccgac cagatccttc aagatcgccg ccttgtcggt cggcacgagc
     3661 accttcatat cctcttttac aacccaggtc tttccacctt cggagaccag cttcgcgctg
     3721 atgaagttca tggccggggt accgatgaag ccagcgacga acttgtttct cggctcgaaa
     3781 tagaccgaat acggatcgcc gatctgctga attatgccgt ccttcattat gacgatcttg
     3841 tcggccatcg tcatggcctc gacctggtcg tgggtaacgt aaatgatcgt tgcctgaaga
     3901 ttctggtgaa gtctcttcaa ctcggctctc atctggactc taagcttggc gtcgaggttg
     3961 gaaagcggtt cgtcgaagag gaagactttc ggatttctga ctatggctct accgacggcg
     4021 accctctgtc tctgaccacc ggagagttgc ttgggctttc tgtcgagaag ctgctctata
     4081 cccaggattc tggccgcttc cttcaccctc tgctcgatct cgggttttgg ggtctttctc
     4141 agtttcaaac cgaaggccat gttttcgtaa accgtcatgt ggggataaag agcgtagttc
     4201 tggaagacca tagctatgtc tctgtctttt ggttccacgt cgtttacgac ttttccaccg
     4261 attttgatgg ttccctccgt gatttcctcc agaccggcta tcattctgag agtggtggtc
     4321 ttcccacacc ccgaaggacc gagcagaacc acgaattctt tgtcgtcaac tttcagattc
     4381 gcatcgataa cggccttgaa accgttcgga taagtctttc ccactttctc aaggatgact
     4441 tctgccatag ttcacctcca ttattaatcg tcaacttcta caatcaaatc ccttatctca
     4501 acgttcatag cttcatcgcc ggcatccata agatacaggt catggaccct ttccagaaac
     4561 ttgttcagca gcacgtactt tccatagtcc ataagaactg ccttggggac tccgtttttt
     4621 gttataatta tatcggcttt ctcgcaatca tcaacgactc tggaaaaatg tgcttttgct
     4681 tctgccagcg aataaaaaga gagttcgtgc aatctggaca aatgaaccac ctcgttacga
     4741 ttatacagta tgaccataat tatagtcaag catagctttc ggaggtgcca aggatgcaga
     4801 agaagttttt ccaaaagcaa cccatgatgc gaaaggttat ctactcgctg attcccatcc
     4861 tgatcttcgc aatcgggaac tttggctggg tcgtcctcat aaaaaccttt ctctcaactt
     4921 ttatggcggt attcatcgaa tggcttttcg aaagaaagaa gggaaaacca gtcagcgagg
     4981 cagtcatcgt tacaggtctt ctggtcgggc tcattcttcc gccggccgtc cccttctgga
     5041 tagttatcgt gtcaagttcc ttcggtgtcg tttttgcgaa gatggccttc ggcggctttg
     5101 cgaaaaacct gtacaacccc gccatggtcg gcagggcctt cgtctatgtt agcttcccgg
     5161 ctgccgttca gcagagctgg actccggccg tcagtacgtg gggccaggga ttcggtcgct
     5221 ggatcgcctc ggacatagtc accatggcaa caccggtgaa cgttataagg ggcgggggcg
     5281 atatgtctat gtggaagatg ttcttcggaa acgtatcggg aacatccggc gaaaccgcca
     5341 agtggcttat catagcggcg gcggtttatc tcataataac caaaactgcc tcgtggaaga
     5401 taatactctc cactctcgtc tcggcttttg tcatgtcggg aataatcttt ctcttcgatc
     5461 cgacccgcct caatcctctg gcgtggatat tcgggggcag catcctcttc ggggcggtct
     5521 tcatggccac cgatcccata agcgctccca agaaggaacg ttcgaagtgg atatacggag
     5581 ggttgatagg ggcgctctcg gtcgttatag gcaccttctc gctctttggt gccggattca
     5641 tgttcgcctt gctcatcgcc aatacctttg cctctctcat cgactatctc aacactccgg
     5701 ggaaggtgaa aaaatcatga aagacaaact gtattcggtg ttttttgcct ttgctctcac
     5761 actcgtcttc acgctggtcc tggccagtat aaacgcgctg gcgagcggca cgatcgaggt
     5821 caacgagaag ctggacttcc agaaatccct gctatacgtc ttcgggtttg tccagaaaga
     5881 cgacaagctt tcaagtaaag agatacagga gctctacgga gagaagatcg tcgagcttca
     5941 cggcgaggta gatgtttacc gggcgcaggt tgaaggagag tctgtctacg ccttcaccgt
     6001 tcagagtccc ggtctgtggg gaacgattac cggcctgata gcggtcaaca ccgacgggac
     6061 gaggctggtg ggaatcgact ttgtgcaaca ctccgaaact cccggtctcg gcggcaggat
     6121 agacgagaag gccttccagc aacagttcag ggatgaaaga atctcggccg gcccttccgg
     6181 cagaattgcg gtggtctcgg gacagaacac ctcttccagc gaggattcga aggtcgatgc
     6241 cataaccgga gccacgagaa cttccgaagc tatccagaaa ctgattgaca aggcaatgaa
     6301 cgaagtcttt ccaaaggtct tgaaggtggt gaactgagat ggctgaatcg aaaaaagaga
     6361 cattcatggc gaacatctgg aaaaacaacc cggtcatcgt acagatcctt ggaatttgct
     6421 cggctctggc cgtgacgaac aacatgacca acaccctgat catgggcatc ggtctgacct
     6481 tcgcgacggg tttctcttca ctcacgatct ctgccctgcg aaagtatata cccaagaacg
     6541 tccgcatgat tgtacaggtg ctgataatcg ccacatacgt cataattctc gatatagtgc
     6601 tcaaggccta cctgccctcg ataagcaagg cgctaggccc ctacgtcggc ctcataataa
     6661 ccaactgtat aataatgggc agagccgagg cctacgcaca gtcccacggg cctgttctct
     6721 ccttcataga cggtgtctcg gccggagtcg gctactcgct gatccttctc ctgatcgcct
     6781 ttatcaggga gcttttgggc tttggcagta tcttcggcct gagagtcctt cccgagagtt
     6841 tcacaccgtg ggtaataatg ataaccgctc ccagcgcgtt cttcatcata ggcaccataa
     6901 tatggatagc cagaacgccg ctggttaaga agaagtgaag gggggagtcg tagatgggtg
     6961 ttccgctcaa tccttttgtc ataatggtgg ccgccgcgct gaccagcaac atggtactga
     7021 gcaactttct cggcatgtgt tccttcatcg ccgtctcgaa agatatcaag acggcgaacg
     7081 gactgggaat ggccgtcact ttcgttctga ctttgacctc cgtcctcaac tatctggtat
     7141 attactacat actggtgccg ctggagctgg tttacctgcg gtacatcgtt ttcattatag
     7201 tgatcgcggc ctttgtccag tttctggaga tgttgatcga ccgcttctct ccatcgctct
     7261 acatgaatct cgggatcttt cttcccctga tcacggtcaa ttgtgcgatt ttgggcggcg
     7321 tgctcttcat gataatacgc gattacaacc tgatgcagtc gctcttcttc ggtttcggct
     7381 cgggtgccgg ctggtggctg gccatagtcg cgctatcgac tatgaggatg cgcttgcgcg
     7441 agaaggatat cccgccacag ctcgtgggac cgggtctgac catgattatc ataggcataa
     7501 tggcgctcgc cttcctcgga ttctccatgg taccggtaca gtgagggggt ggcgcagttg
     7561 caaatactta taacggctct cgtactgggc ggaatatcgg ccgtcctggc ggcgattatt
     7621 tcggtcgccg atgccgtgct aaacaactac ggcgatgtga aactcacggt gaacggttct
     7681 aagtcttaca cggtgaaggg cggcagtacg ctgttgacaa ccctggccgg cgagaagatt
     7741 ttcgtcccat cggcctgtgg cgggaagggc agttgcggcc tctgcaaggt caaggttctt
     7801 tcggatatcg gtccgattct cccgacggaa ctaccgtacc tcaccaccaa ggagaagagt
     7861 gaaaacgtac gcctctcctg tcaggtgaag gtcaagggag acatttcgat agagatcccc
     7921 gagggacttt tcaacataag ggagtacgca tccacggtct ctacgataaa agatctgacg
     7981 cacgatataa aagaggtctt cttcagcttc gatgacgata taaacttcag agccggtcag
     8041 tacgtccaat taatagtacc gccctacggg gagatcaagg agagtactat gagggcttat
     8101 tccatgtcgt cgcagccttc gataaagaac ggtgtcgagc tcctgataag gctggtgccc
     8161 aacgggatag tcacgacgta cgtacacaag tatctgaagg agaaggacga ggtcaggctt
     8221 ctggggccct ttggcgactt ttttctgagg aacaccgatg ccgacatcat tttcatagcc
     8281 ggtggttccg ggatggcgcc gatcaagtcc ataatactgg acatgaaaga aaagggtata
     8341 aagagaaacg catactactt tttcggtgcc aggagcaaga aggatctctt ctaccttgac
     8401 ttgatgagag agatcgaaaa agaagtgccg aacttccatt tcataccggc tctctccagc
     8461 cctttgcccg aggataactg gcagggtgag ttcgggctca taaccgatgt ggtggacagg
     8521 tacctgaccg aacagggtga gatagaacgg gaaggctatc tctgtggaag ccctggcatg
     8581 ataaacgcct gtataacggt acttggaaag cacaaaattc ccgaaaacag gatctacttc
     8641 gacaaatttg gataaatcgc gggggggtga ttatatgaag cagactccag acctgggtaa
     8701 agttcaggaa aacatgaagg ccgggcagat aaccgggccg ggcttcctcg gcagcgacga
     8761 gagaaacctg atagatataa tccgggaaga tgaacagaag atcagcgaat tgggacttac
     8821 caacgaaata atagccgaca agctcgaaat gctgatgcga cagggtgaga agggcttcgg
     8881 ctctcccatt gtagttgaca accgttttgt cgtgatagtt gacgagagca ggggctacgt
     8941 tccatgcccc ttcagacatg ggaatctatc caagaaggtc aacgtcaacg tgcggagcat
     9001 ggccttgaaa aaggagatcg actacactcc catatcaatc catctcataa gggagcacgg
     9061 cttctaccag ggcaagggat cgctatacag gctcgatcct gaagaggtgg ctaagtttct
     9121 cgaattgatt tgataccccc accgcccgtc gcgagaagac ggaatacacc ttacctctaa
     9181 gtccgagcct cgacgaggcg gtgaacgatg tatggaggat agtgttttga agaggagact
     9241 tccgaacaga gcgatattca ccttcgtctt cattatcgcc ttgatttctt ttgtggcgta
     9301 tttcgcctcc aaatacagtg tgaaagtggt cgtaccgacc gatgtatctc tggacgaact
     9361 gcttttcaac gccgagcatt tctcgcttct cggggaggcg gagtatccac ccgaccaggg
     9421 acttgccaac ggcatcgctt atgaagatga gaagggaata attcatcagg tgacttaccc
     9481 ggattacgat gactcggttc gcgggtatct tctcgacctg gattccggtt cgaacgtcat
     9541 aaaggttttt gtctggaata gcggcagtgt aacccaggcg aaagagacct gggaacggct
     9601 ctacatgatc gaaggctccg tgatgaccag ggagaggggg cgaataaaga aggccgggta
     9661 cttctacgcc cgcataatca ggttcggcgg aaaggatgag acacttctgt ggcaaaagga
     9721 caggtgggta gttctggtaa agtcgccggc ggttctcgaa aaaaagaccg aagagataat
     9781 tttgacagga ctcttcgatc ctaaagtaag aagttgatct cggattccgg ataaacttcg
     9841 ataccgttct tcaagagaag ctccgctgtt attcccattc catctactgt cacgccgctg
     9901 aaggtgccgt cgtatatccc tctgcatccg cacgaggggc ttttcgattt caggaccgca
     9961 atgccggtgc tgcaaaatct ggctatcttc agaacctctt cggcgcccct gtggaactga
    10021 gcggttacat cgtggccgaa ctggtcgata acgattcttc tcgtcgtcca gtcgttcgag
    10081 acggtgatct cggccttcga tcttggcgtg ggaagtccac ccagctgttc gggacagacc
    10141 gggaacaaaa taaacctgtc cacaagcctc aggagctcaa gggacaggtt gttatctcca
    10201 cgataggtac agttaattcc gaccagacag gcacttacga ttatcctgtt cattttcctc
    10261 cggcagccgt tccgtcgtct tctctcctta tgacgtacat gaggtcgcgg gtaggcaaca
    10321 tagaaagcgg atcgagcgtg ttcatcttcg ggtccttcac ctcgaagtga acgtgcggac
    10381 cggtagacac tcccgtgctt ccgacacggc ctatcagaga accggcatcg accctctgtc
    10441 ccacatagac agatatcttg ctcatgtgtc catagcgggt gacgtaaccg ttatcgtgct
    10501 gtatatccac ggcgttgccg taacctccgt tgacaccggc ggcgatgacc acgccttttg
    10561 aagccgcgaa tataggcgtt ccctgcggag cggcaatgtc cagcccgcca tggaaggagg
    10621 ctcctccgtt caccgggtgg gtacgccagc cgtacgagga agaaataacc ccgtaaacgg
    10681 gccatctata cggggtggtg tgtggaacgt attgatacat atttattaga gtcataggaa
    10741 cgaagattcg ctgtcccggg cttatcagcg aactagagtt gaggttgttc gccgatagaa
    10801 gctccttgac gggagcgaag aacaacttcg cgataccgtc tattgtatct cccgattgaa
    10861 cttcgtaaat caaaccgtcg ggttgaggca gtttcaacct gtcgccggct ttgatgttgt
    10921 tgggatcgct cagattgttg aagtcgatta tggttgacat gctcactccg agctccctgg
    10981 agatagtgtg aatcgaatcg cccgacctca ccacgtaagt gacgagaaaa taagcaaagc
    11041 tacgagtcac taatagcaaa gacacgaaaa gaagaactag aatgacccga cggttcattc
    11101 cttgccacct ccaactctaa aaaggccaaa agaaaagaag aaaaccacaa ttattatacc
    11161 aaaaagaata ccaacggcaa aatgcaaatc gaaaatttct ttgatcaaaa ttgaaagacc
    11221 tatgagtacc gacatcgccg atcccatcag tactatcgcc gccgctacgt ctttcaggac
    11281 ctttacgcgt tcatcgtaat acgggtgaag aatatccgcg atcttttcta caacggtgtt
    11341 aacggcctcc agtattacaa ccataaaggt gcagaacagc agccacaaaa aatcctgtct
    11401 ctccagccgg atcagggctc cgatcgttag aacggcgccg ccgaagacca gctgtattct
    11461 aaagttcctc tctttcagca aataacccag gccatttgcc gcgtggtgca aactttttag
    11521 aaacttcctc attcaaatcc ccggcccaca gagtcgattc tctcgcttct gaggttgtag
    11581 gatctctcta taagcgaata accgaaatct atagcctggg aattcatatc gacgtacttg
    11641 gcctttatat gttttgacat ggccaccttg agagcctggg tggtaacgaa gtttccggcc
    11701 ctcgctatca ggccgagtcc gaccatattt gccggaaggg atgttccgaa cttaccttct
    11761 gtgagttcgg tgaccggttt ggaaatgatc ttcctggtca tcctagctcc ctctttgggg
    11821 atcgatttga cctgagtaga atcgattatc aatattccat tcatgtgcag tagagggaca
    11881 tgcgcgttga cggccatctg gtgcatcgag tatatgactt cgaaggagtg ggccctcggg
    11941 tagtcgatcg gttcggtgtt gaaaagaaca tcgcagtatg aaagtccgcc gcgcacctgt
    12001 gcgccgaaag actgagtctg taccacccag cttccctggt ctagaagagc ctccgccagg
    12061 ataatgccca tcaggacgtt cccctggccg cctattccag aaatcctcac ggtatggggt
    12121 tgaggaaatg ccttcttcat tctctttcac caccctctgc cgcgagcact ttttctctca
    12181 tcttttcgta cctgtctatg tacgtttcag tgtctacgtt cacaaattca ccggcaacga
    12241 ttttatcccc gatctcttcc gggctcatgg ttttggcttt ggcaagctgg atcgtgttgg
    12301 ttttgaaata gttcagcata tcctgaggtt tggagaggct gttgtacctt ccgtaataag
    12361 tatgacagtt cgtcatgatc tcgatgacgg ccattccctt gtgtttcaat ccgttccgta
    12421 tgaattgaac ggcctgtgcg tagtgaataa ccgtggaacg ggccacgtaa gtcgccccgg
    12481 cggccatggc aaccttcact atgtcaaagg agttttcgac gtttccgtag ggcgaggttg
    12541 aggctatctt ctccgttggc gtagttggag agtactggcc gcccgtcatt ccataaatgt
    12601 tgttgttgaa tactatggta gttatatcca tgtttctcct gcaggcgtgt atgaagtgat
    12661 ttccgcctat ggccatcata tctccatcgc cgcccatgac aataacgttg aattcgggcc
    12721 gggcaagctt cacgcccgtg gcgaaggcaa cggctctgcc gtgaagtgtg tgcatcgtat
    12781 tgaagttcag ataaccggtt acccgtgaag aacaacctat tcccgaaact acggcgattc
    12841 tatcggggtc gagcgactcc ctgtccaccg catcgacgaa tgccttcatg atgacaccgt
    12901 ttccacaccc gggacaccac acgtggggca ttcgatcctg ccttaaatac ttcgtaagtc
    12961 tggagacagc catgatttcc tccctttcac attgagtaat ttatcattat gactcctgtc
    13021 ctatcgaaca tgaattccgt gagcgggtcg gggtagcctt ccccgaatac caccctcttg
    13081 atgcctgcgt taattatcag tcttgcgcag atcgaacaag gtctgtgtgt aacgtatatg
    13141 gtagaaccgt tggtggctat tccaaaacgc gcggcctgcg agagggcgtt ctgctcggcg
    13201 tgggcggcgt aacacaactc ctgattctgt ccggacggta tgttcagcgc gtctcttata
    13261 cattcggtct cgtcgcagtg aggaaaaccc gacgggggct ggttgtagcc cgtcgccagt
    13321 atcctgttct cgcgcactat cagggcgccc acttttctgt ggtggcagct cgacctctct
    13381 ctcacctggt aggcgagctt catgaaatat gaatcccagc tttccgaagg tgtcttggat
    13441 ttatgcgtct tgaagccgtt aagaaacttt tcgagttctt cgccagacat ttcataagtc
    13501 ctttcggtga ttctacgtat ttttttgctt ctcgactgtc gctctggaag ggagaaggct
    13561 ctccatctct tccattatac cgaccaatct ctcgaaaact agcctcgaca tatccagtcc
    13621 gtagtcattc aaactgagcc tgtatccatt gacaaccagg aactctccaa ggaccgatgc
    13681 gattctttct acacttctct ccaacaggtc ggccgggaag atctccgccg atagagtcaa
    13741 accgtcaatc agcctcaatc ccatgaagag cgtttcgaag agctcttcaa gcggtgtatt
    13801 ctcccgtata taagccaaag gacgtttgcc agcgactatg gagtcgatgt attggtccag
    13861 agagtcgcag ttcacacttc tggtagtgcc ttcgtggctg ccggcagaga ctccgaaacc
    13921 cttgtagtct ccgcttttcc agtatttcaa attgtgtaac gacatcctgc ctttcgacca
    13981 cgaagatatt tcatatctgg ttaacccaag ctctttcaga gataaaacga taaaatcatg
    14041 cagcttctcg agcgtgtctt tgtcgggcaa ctctatgata tcattcttaa ccgatgccat
    14101 caaatcggtc tcatggtcac tatcgaacat ataataggaa atatgagccg gcttcagcct
    14161 ttctatcaat ttaagattgt tttctacgtt tttgcggttt tctccgggca atcccagtat
    14221 gaaatcgaag tttacattat cgaagagatc gaccgccatt tccagtctct caaacagatc
    14281 aggaggtacc ggtctgttga ctgtcttaag gatctctcca tcgcttgact gaagaccgac
    14341 cgagagtctg ttcaccccga gctttttcca tctctccagt ctttcgaacg tcaattccca
    14401 ggggttggct tccagcgtgg cctcgcgcaa ttcgaacctg aaccgggaac ggagagtatc
    14461 aagcagctcc tgcaacagat cgagcggata gagcgaaggg gaaccgccgc cgaaataaag
    14521 cgtatcgacc gcggcgccgt cggaaaagag ctctatctca ctcttcagcg ctgcatggta
    14581 cttcttcaga gaggtttcgt caagctgtaa cgtacaaaaa tcgcagtatg cacaccgtct
    14641 ggcacagaag gggagatgaa gatacacccc ggcgtacatc agaatatgag cctcatgaaa
    14701 accccgttga gtttgaaatt ggtgatcgaa attccggcgt acaccgagtc gtcgcttccc
    14761 agtttagata tcagcaactg ggcgtcaatt cctctgaggc tgaccatgat cattggtgag
    14821 agaaccagct tcaaatcgcc cgcaggaacg atgaaattcc ccgaaaaggc ggcgcccagg
    14881 tcgaagctca tataattcat gccgactccg aagccgtaac ccatctcacc cagcgaatcg
    14941 ttcgtcttca aatccacggt caccaccacg ttcgtttcgg catttcgcag gcggagactc
    15001 ctttcgaatt ccttcgcgta gagatagagc ccgatatcgt tgaaggcgaa atcatcgact
    15061 ctgtatatga acgtgctgag accgccgttt atctggccca gaggtagtct gaaccagttg
    15121 cggaccagat acctcctgtc ccccagcgag gagagctcga gttccagaag gctgtgcgaa
    15181 aagaccggaa tctggttggt gttggagacg tacctcgccc ccatgtataa tccttcgtgg
    15241 ctcgcgagga tcgagccgcg aaaatcgccg tctctgattt tcatcttcaa gaaaccgctc
    15301 gaaaactcct cccagtgatc tttgtaggtg atcccgacgt aaggcatccc agaagccagc
    15361 gcgagtgtca ggtggaagtt ctcgtaggct tttatatccg tagaaaggct ccatcccctg
    15421 ttggtgaact gtatctgtgt ctgggaaagc gacagcgagc atacaagtat cacaataagg
    15481 aaacaaaccg tctttttcaa tccttgagca acctcctcgc catttcattt accctcgcgg
    15541 ggttggcctt cccccgtgtc gccttcataa ccgcgcccac gaagtaaccg agaacgccct
    15601 ctttgccttc cctgaattgc tggacggcct ttgggttgct ttccatggct tctctaattg
    15661 ccgtttcgat aacaccgtcg tcgtttatct gctcgagccc cttctctttg actatggaag
    15721 ctggcgaacg ctttctctcg atcacttcgg ggagtatctc cttggcgata ctgttggata
    15781 tcctgccgga gtcgatcatt tcgaaaagtt ctttgaagta ttccggtttc agcccgagac
    15841 tctctataga aagatctccc tcgttcatta ttctcattac ctcgcccatg atccagttcg
    15901 agctctcttt gggcttgcct gtgacccgcg ccacttcttc gtagaagcag gctacctcgc
    15961 cactggaaga gaggaccgag gcgtcgtact cgggcagtcc atactgttca atgaatctgc
    16021 gcagcttctg ccagggcagt tccggcagag actccctgag tcgtccgatt ctatcctgtg
    16081 ttatcatcag caccgggagg tcgggttcgg ggaagtaacg gtagtcgttc tcctcttctt
    16141 tggagcgcat cgaaaaggtc tccttggtag cgaagttcca ggagcgggtc tctttggcca
    16201 cgttgccgcc ggcgtcgagc acggccgata ttctctcttg ctcggattcc agtgccttct
    16261 cgacgaactt gaaggaatta atgttcttga cttccaccct gttggaactc agaccggaag
    16321 cgtcgtctct catcgaaatg ttggcgtcgc atctcaacgc gcccttctcc atgtctcccg
    16381 agcagacgcc cagggctctg agtgtgtctc ttagtagctc catgaagacc ttcgcctggg
    16441 ccggcgtctc gagatcgggt tcggttacta tctcgatcag gggaactcca cacctgttga
    16501 gatcgaggtg actgcctgtg gagccggtta tcgaatcggt tccctggtgg atcatcttgc
    16561 cggcgtcctc ttctatgtgg attcgcctta tcctcaccct tttccctact cctccgactt
    16621 ctatatcgag ataaccgttt tcagcaatcg ggaagaaata ctgggtaatc tggtagccct
    16681 tgggaaggtc ggggtagaag taatttttac gatcgaagac cgaccgttcg ttgagtgtgc
    16741 agttgaaggc cagtccggcc ataatggcat aatccaccac gttttcattg agaacgggaa
    16801 gcgcacccgg ttgaccggtg caaacgggac agatggccgt gttgggttcg agctcgaaaa
    16861 catcggccgg gcaactgcaa aaggccttcg tctttgtctg aagctgtgca tgtatctcca
    16921 gtcctataac ggttctgtac atcattcaac ccacctttcc gatattctgg tcaggccgcc
    16981 ttcgtatgcg ggagaaaagc cctcgatgaa actggccgcc tccagaagtt taacgtcgct
    17041 gaaccgcccg cctgtgagaa gcatacctgc aggtatgctt ctcacaggcg ggcggt
//
LOCUS       DAOOLY010000018        16313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3759_length_16313_cov_4.484377, whole genome shotgun sequence.
ACCESSION   DAOOLY010000018 DAOOLY010000000
VERSION     DAOOLY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 16313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16313
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3759_length_16313_cov_4.484377"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLD15_01855"
     CDS             complement(<1..293)
                     /locus_tag="PLD15_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Inner-membrane translocator"
                     /protein_id="HPB62672.1"
                     /translation="MAILEQGLIIGLMALGVYISFRIVDLPDLTTDGSFALGAAVAVR
                     ALVTGIPAWLAITLAAIFGAVAGSVTGMIHRKFKVNVLLAGILVMTMLYSVNL"
     gene            complement(298..1269)
                     /locus_tag="PLD15_01860"
     CDS             complement(298..1269)
                     /locus_tag="PLD15_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62673.1"
                     /translation="MKRVLVCVVLLLGVLGLSLQTVAITAIVEHPALDSVREGIIDVL
                     AENGFKDGENIKIDFQNAQGSVSTAVTIAKQFISLNPAVMIGIATPSAQALVNATKTI
                     PVVFSAVTDPVSANLVPSFGKNPGNVVGISDMTPVKTQIQLLKLAIPDVKTIGVIYNS
                     GEANSVTIRQFAKEACDALGLNLIDITGSTTVEMVASLNAQIRDVDAIYIGTDNTAAS
                     SMESISTVALREKVAIVAADIDIARTGGLIGFGFNYYQIGRSTGDLVVQILNGVSPSE
                     LETKILGQESLILFVNTDIAEQLAINLPQSILDRADLVVEDGVEKTR"
     gene            complement(1527..2465)
                     /locus_tag="PLD15_01865"
     CDS             complement(1527..2465)
                     /locus_tag="PLD15_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62674.1"
                     /translation="MNDLPSFVISTLQATVRAGTPLLFAVLGTIFTERSGVMNLGLEG
                     LMLVGAIGGFMASYNTSSLVAAVLVAMLAGAVLGLIHAFFTVTLKINQIVSGLAITML
                     GTGISGLWGKDYIGVIARRFNPIKIPLLSDIPYIGRIFFEHDMLVYISFVLVPVMWFV
                     IYKTKAGMTLRAVGESPDAADAKGINVFAVRYLFTILGASITAIGGAYLSLAYNSMWI
                     ENMTAGRGWIAIALVLFATWDPFKALIGAYLFGGITALGLRLQAIGAHISPDFLKMLP
                     YLLTIAVLFFTSTDALKRKVGAPASLGVPYSREERT"
     gene            complement(2458..3570)
                     /locus_tag="PLD15_01870"
     CDS             complement(2458..3570)
                     /locus_tag="PLD15_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62675.1"
                     /translation="MKIRIEKRLEIPRYAGIIVPVISVAVALLIMGIILLVFFVGRLG
                     NLSSAFRETVEAYKEMLTWPFANRYGLFSTFMRMVPLAFVALGLSFAYRMKIWNIGGE
                     GQLYMGAMVSTWGALFLFKDVKSPLIMIVLLSIVGAAAGAAWALIPALMKAFARTDEI
                     VVTLMLNYVAIFWVDYLVYGPWKDPKGYGFPGTAPFPDQARLPSFFDDEFHIGVFIAL
                     AVAVLLQYIYRKTGWGFNSQIVGDNLLAARYSGINVRNYTIIALVVSGAIAGLGGAVQ
                     MMGVQHRLQHGFSPGYGYTAIIVAWLAKLNPLAILLVSFLFGGLLVGNDQLQMFYKLP
                     MALISVFQGLVLFCLLGGEAVSKYRLAIVKEVPENE"
     gene            complement(3572..5104)
                     /locus_tag="PLD15_01875"
     CDS             complement(3572..5104)
                     /locus_tag="PLD15_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62676.1"
                     /translation="MISLEDFPVLEMKHISKSFPGVMANDDISLQLKKGEVHALLGEN
                     GAGKTTLMNILFGIYHADRGEVFINNGKVSITSPSHALKYGIGMVQQHFTLVPSFTVA
                     ENVVLGLKDFGFVIKTKTVESKIKELSEAYGLPVNPRARVWTLSVGERQRIEILKLLY
                     TGAGIIILDEPTAVLTPQESDMLFKAIRKLVSEGSSVIFISHKLDEVLAISDRITVLR
                     GGKVVGTVEREKIDRRGLVRMMVGRDVLLDQEKPVVETGRAILKIENLNVKNDKDLMS
                     VRDLSLEIRSGEILGIAGVAGNGQRELAEAIYGLRKKVSGRIELDGHELRQGNVAERI
                     RAGLSFIPQDRKTMATCPNLSVMSNLFMKNSICELVHCSPWKLDGRRMHSKADELIGE
                     FSISTPSAKTEVRYLSGGNLQKVVLARELSNEPKVIIAEHPTRGLDIGAMEFVYKSLL
                     SQKERGAAILLLAGELYEIFRLSDRVAVIYEGQIMGYTPPDPSYIEEIGFMMAGARQE
                     DH"
     gene            complement(5147..6199)
                     /locus_tag="PLD15_01880"
     CDS             complement(5147..6199)
                     /locus_tag="PLD15_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB62677.1"
                     /translation="MKRFLLILTLLLVTTAMLAAPLRVAFIYVAPIGDGGWSFMHNQG
                     RLYLEKVFGSDIVTDFIENVPEGAEGESVFRGYAQRGYDLVFGTSFGYMDSMVKVSKS
                     FPKTTFMHCSGYQVTDNLGTYFGRMYEPRFLSGLVAGAMTKTNIIGYVAAFPIPEVIR
                     GINAFAMGVQYVNPQAKVHVVWSNTWFDPAREKEAAVSLLDIGADVIAQHQDSPAPQQ
                     AAEDRGVYSIGYNSDMSAFAPKAFLAAPVWNWGPYYEKIVRSVMDGTWTNAQYWGGIN
                     DGAVDIVLSDLVPSGVAKLVMSMRNAIVKGDMHPFVGPIYDQAGNLKYAEGEIPSDGD
                     LLSMDWFINNVVGKIQ"
     gene            6456..6941
                     /locus_tag="PLD15_01885"
     CDS             6456..6941
                     /locus_tag="PLD15_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPB62678.1"
                     /translation="MITTRMEKELNAQIKAEYESAFIYMAMAVWAHKNAYFGTANFMW
                     KQAKEEEEHAMKFVGYLKDVDGTIEIPGLEKPEGEFKSLLDIFEKGLEHEKYITSRIH
                     NLVTVSDEEGDYATNDFLQWYVTEQVEEERNFRDIVKLLKKVGDHTNGIMMIDAKLGE
                     R"
     gene            7033..8835
                     /locus_tag="PLD15_01890"
     CDS             7033..8835
                     /locus_tag="PLD15_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB62679.1"
                     /translation="MRKLFLAMFVVISLAVLVFATQVNLSIIHTSDIHGNIFPVDYAT
                     GKFSAVGLAKISAFLSEMRKSNPNTLAIDTGDLIQGTPLAYYYAKVDNSGENPMIAAL
                     NHMGFIASVIGNHEFNYGPSILERAIGEAGFPMLSANIVFESSGEAAFAPYVIVEVPF
                     EGDTIRVGVLGLTTSFIPNWEEPKHIAGLNFLDPVSTALEYVPQLREKVDVLVVAYHG
                     GFERDLKTGAPTEELLGENVGYQILKEVKGIDVLLTGHQHRTIAEVIDGTVISQPSNW
                     GKVAGKIDLVLEKKNGKWEIVNRNIAQISMANYDADSEVMAMFQNFEDRVQKWLDQPV
                     GIAIGDFYIDDPFVARLADNSLIEFVNRVQSFVTGAPISSVALFTNDIKGWKTGPITI
                     RDINAVYIYPNTLKVIKVTGADIKAALEKSAEYFTYEDGEIVVTDSWINPKPLHYNYD
                     IWEGIEYIVAADRPVGERVLSVRYMGRPLDMDGEYEVVLNNYRAGGGGGYTMFQGKPV
                     VREVMLEVSEIIADYVLERVAVSATLDRNWSVGTGLIHTVGWNETLRTISEMYGIDAS
                     EIMKYNPDLKRVERIPAGTKIIIYRPTLEEVPVI"
     gene            complement(9082..9990)
                     /locus_tag="PLD15_01895"
     CDS             complement(9082..9990)
                     /locus_tag="PLD15_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HPB62680.1"
                     /translation="MGENGKRAEGFVEVKGGKIWYCVEGADKSGIPLIILHGGPGTPH
                     DYLEPLSALSDERPVIFYDQLGCGRSDRPSDKSLWTVEHFVGELNLLKERLRLGRVHI
                     LGQSWGTILACEYYFFKPEEVVSMVLSGPAMSIPQFEKDVRKLLAFLPKERQNAIHRA
                     EASGRFDSREYADAMRTFYNRHVCRLDPWPDCLNRTLEGMGTAVYEYMYGPSEFTVTG
                     TLRNYSCVHRLGEIEVPVLLTCGRFDEATPSTIELYRREIVNCEMKIIEDASHEHHLE
                     KPEEYMETVRSFLKKVESHGPRKYFE"
     gene            10118..10462
                     /locus_tag="PLD15_01900"
     CDS             10118..10462
                     /locus_tag="PLD15_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB62681.1"
                     /translation="MVDCCYEHENKTDTAFLRLKASEVSGLSKLFRVLGDETRTRIVY
                     LLSITELCTCDLARVLNLTLPTISHHLKLLKELRLVRSRREGKAVYYALADEHILVLV
                     KTAREHFEEESH"
     gene            10459..12513
                     /locus_tag="PLD15_01905"
     CDS             10459..12513
                     /locus_tag="PLD15_01905"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPB62682.1"
                     /translation="MKTKHRIKGLDCPVCAAKIEDRIKKMGYSAKIDLASQSLIIEDG
                     DIEEINRIISSVEPGAGITVDSGGSTVTRKFGILLLSCVVYLFALITGRYLHGRYEPL
                     EYGIYILAYILAGNGVLLKAGRNILHGRLFDENFLMTLATVGAFLIHELPEAVAVMLF
                     YNAGEILEGMALARSRKSISSLVDLKAETVNRVTPEGLAAVPASSMVAGDRFVVKPGE
                     RIPLDGSIASGETWLDLSLLTGESRPVKAGEGYGVVAGTINSTGLIEVEATASLERSY
                     TSRMLQMVEEASSRKSRTERFITKFSRIYTPIVVLIAAFLATVPPLFTGGNYGEWFHR
                     ALILLVISCPCALVISVPLGYFAGIGKASRYGVLVKGSNYLEALAGIDTVLFDKTGTL
                     TEGRFGISEISGHNGMAPAEAMLIAASVESNSNHPIARSIVDHAFSIGFKKEYLLAVS
                     DYREIPGEGVHATVEGKEIALGNERILRGSTILPAAKGKSKIYLTVGGKLSGTIDISD
                     RLKEDSKMAIESLRDQGLASIAMLTGDSPDVAAAISGELGLDWYEGGLNPEGKLETLE
                     RSSRTGCKVAFVGDGINDAPVIARSDVGIAMGRIGSDAVIETADIVISDDSPLQVSRA
                     IAIARRTRSIILQNICLAIGTKALFIVLGAFGLVSMWGAVFADVGVALLAVLNSLRVF
                     RD"
     gene            12536..13756
                     /locus_tag="PLD15_01910"
     CDS             12536..13756
                     /locus_tag="PLD15_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62683.1"
                     /translation="MKPEKLYYLYSGSVYFLFNLVFTINGIYFVESVGLNPLQLVLVG
                     TVVETSALLFEIPTGVFADMYGRRRSIITGLTVLGVSFILEGSLPFFWTILLAQIIAG
                     AGYTFLSGADIAWVADESGIENLDSVLVKGAQFEQIGSLAGIWASVLLGLFALNFPIV
                     LSGVSMFLLALILRRIMKENKFRPKHRGKPLKDFLSTLADGLGQIRTSASLTIILFIS
                     LGIGLYSEGFDRLWILKFLEDLKITEEFGLRYVLWFGVINSGAMVLSIAVLSFLKRLS
                     SRNSSKLLLSYLVILNTILAVAFIGFALSGNFPLALAFYWAGFVARRNDYPLYASFIN
                     RQIEVSGVRATVISVARQFDSLGQIIGGPIIGLIALSSTTLALGVTGLLVFPVVLLYI
                     WLLRRESRPDRTGK"
     gene            14154..14999
                     /locus_tag="PLD15_01915"
     CDS             14154..14999
                     /locus_tag="PLD15_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPB62684.1"
                     /translation="MKKTLVLIPLVFVLLFLSTGCSLIFTTRINSQYEKEIDILETKA
                     LKVTGTNGSLTVLVWEKDYVRVTGTKFVSGLNSSQVKAFNNSLDVVWSNLGETLEIGL
                     SRTDRPGGINYGVSMTVYVPRELYESHFYKTSNGSVSISGISGECVIESSNGNISITG
                     FEGSIYSVTSNGGYKINLCKLYGDNYFKTSNGSVVAKLLTSPTGRLEIYTSNGKIDFK
                     LPWTAGAFLTAATSNGNVDVQGFRSISFEKIDKNDLRAEINNWRDLDLSLRTSNGDIT
                     LNGMQ"
     gene            15142..15765
                     /locus_tag="PLD15_01920"
     CDS             15142..15765
                     /locus_tag="PLD15_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62685.1"
                     /translation="MRKVMLTVAILVLFVSFTVALVGGSQVPEFFSDWRFLRAHVFSD
                     YVVYELMQTDMSGFEVKVVQAGPTGGLKIGFFSKSVLVAESGPNFFMGLWNDVLMVDT
                     GTAPEPRLLSLYHIPEKRLVHRDSYFSPVEIDGERLLYYRDIGLRFYDPVNEKRLPEE
                     LAFLEESLVSMLKDQPYLGVAISEKVYLDLLTFELVNTGEYVIRYVQ"
     gene            complement(15746..>16313)
                     /locus_tag="PLD15_01925"
     CDS             complement(15746..>16313)
                     /locus_tag="PLD15_01925"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HPB62686.1"
                     /translation="FYNLLHNEDARFRLKSGGLCREHSRRIVEMAREKSDIGSLPVSV
                     VFKELLFGQLNLIGPGERKLFKRVSSSRNTGGCHLCEVSRESEKRYVIAIAKVFDSEK
                     IRKLYEESSSILCIDHAREVAASMRAATVEWFVTLQKSKIESVIEMLERYIGKNDYRN
                     TSPIGEERNAWLTASRIIGETRVTGRNE"
BASE COUNT         4039 a         4143 c         4161 g         3970 t
ORIGIN      
        1 ctgagattca ccgaataaag cattgtcatg actaggattc cggccagcag aacgttgacc
       61 ttgaacttcc tatgtatcat tcccgtaaca cttccagcca ccgctccaaa tatagccgcc
      121 agcgtaatcg ctaaccaggc gggtattccg gtaaccaaag ctctaacggc gacggcggct
      181 ccgagggcga aagagccgtc tgtcgtcaag tcgggaagat caactattct gaaagagata
      241 taaactccca gggccatgag gccaataatc agtccctgtt ccaatatcgc catcagatca
      301 ccttgttttt tctacgccgt cttcaactac aaggtctgcc ctgtccagta ttgactgagg
      361 aaggtttatt gcgagctgtt cggctatgtc tgtattgacg aaaagtatca gtgattcctg
      421 tccgagtatt ttggtctcga gttccgacgg gcttacgccg ttcaggatct gaaccacgag
      481 atctccggta gatctgccta tctggtagta gttgaaacca aaaccgatca gtcccccggt
      541 tcttgcaata tcgatatcgg cggctactat ggcgaccttt tccctgaggg cgacggtgga
      601 aatcgactcc atactggatg cggccgtgtt gtcggtccct atgtatatgg cgtccacatc
      661 tcttatctgg gcgttgagag aagccaccat ttcgacggtc gtagagccgg ttatatctat
      721 gaggttcaat ccgagggcat cacaggcctc ttttgcgaac tgcctgatgg ttaccgagtt
      781 ggcctcgcct gagttgtaga tgacgccgat cgttttgacg tcgggaatgg cgagtttcaa
      841 gagttgaatc tgtgtcttta cgggggtcat atcgctaatt cctactacgt tgccggggtt
      901 ttttccgaag gaaggtacga ggtttgcact tactgggtca gtcaccgcgc tgaagacaac
      961 ggggatcgtt tttgttgcgt ttaccagagc ctgggcggaa ggtgtcgcta ttcctatcat
     1021 caccgcaggg ttcagagaga taaactgctt cgcgatcgtt actgccgtag aaaccgaacc
     1081 ctgagcgttc tggaaatcga tcttgatgtt ctccccgtct ttgaagccgt tttcggccag
     1141 aacatcgatg attccctccc tgactgaatc cagagccgga tgttccacaa tcgccgtgat
     1201 tgccaccgtt tgcaacgata atccaagaac acctaaaagt agtaccacac atacaagaac
     1261 ccgcttcatt ctacatccct cctgatataa aaagagagag aggacgtttc tcaacgtcct
     1321 ctctctctat tctccttcgt gtattttaac tcaagcacac actggaggag tccgaagaaa
     1381 ggacgttcct cctaaaccaa taagcgtatg caaagcttac cagggttgcc cgaatcatca
     1441 ctgtttacct ccaaaaactt cgtttgatat tttataccac aaaacaatgt tacgggtcaa
     1501 ggcagtacat aaaattattt ttttcgttac gttctttctt ccctcgagta aggtacgccc
     1561 agagaagcgg gggcaccgac ctttcttttg agagcgtcgg tcgaggtgaa gaagagaacg
     1621 gctatcgtga gcaagtaagg tagcatcttc aagaaatccg gggagatatg ggcaccgatg
     1681 gcctggagcc tcagccccag ggcagttatt ccaccgaaga gataggcacc tatcagagcc
     1741 ttgaagggat cccacgtggc aaacagcacg agtgcaatgg caatccaccc cctcccggcc
     1801 gtcatgttct ctatccacat cgagttgtag gctagcgaga gataagcgcc gccgatcgcc
     1861 gtgatggagg ctcctagaat cgtgaagaga tacctgaccg cgaaaacgtt gattcccttt
     1921 gcgtctgcgg cgtccggcga ttctcctacc gctctgagcg tcatgccggc cttggttttg
     1981 tagatcacga accacatgac cgggacgagc acgaagctta tataaaccag catatcgtgc
     2041 tcgaaaaata tcctgccgat gtagggtatg tccgacagca ggggaatctt tatgggattg
     2101 aatcttctgg ctataactcc tatgtagtcc ttgccccaga ggccgctgat tccggtgccg
     2161 agcatagtta tagcgagtcc gctcacgatc tggttgatct tgagcgtgac cgtgaaaaaa
     2221 gcatgaataa gtccgagcac ggccccggcc agcatggcta ccagaacggc tgcgacgagg
     2281 ctcgatgtgt tgtaggatgc cataaacccg cctatggctc cgaccagcat cagcccttca
     2341 agcccgagat tcattactcc cgatctctcg gtgaagatcg taccgagaac ggcgaaaaga
     2401 aggggggttc ccgccctcac agtcgcctgt agagtggata ttacgaagga gggtagatca
     2461 ttcattttcc ggcacctcct ttacgatcgc aagcctgtat ttggacacgg cctctccgcc
     2521 aagcaggcag aagagcacca ggccctgaaa gacggagatg agagccattg gaagtttgta
     2581 gaacatctgc agctggtcgt taccgacgag cagacctcca aagagaaatg agaccagcag
     2641 tatggcgaga ggattcaact tggcaagcca ggcaactatt atggccgtat aaccgtagcc
     2701 tggcgaaaat ccgtgttgca gtctgtgctg aacacccatc atctgcaccg cacctccaag
     2761 accggctatc gcgccgctga caacaagggc gattatagtg tagttcctga catttatccc
     2821 cgaatatctt gcggccagca ggttgtcgcc gacgatttga gaattgaaac cccagccggt
     2881 cttcctgtat atgtattgaa gcagtacagc gacagccaga gctatgaaga cccctatatg
     2941 gaactcgtcg tcgaaaaagg agggaagcct ggcctggtcg gggaagggcg ccgttccggg
     3001 gaagccgtac cctttcgggt ctttccacgg gccgtacacg aggtaatcga cccagaagat
     3061 ggccacatag ttcaacatga gagtcacgac tatctcgtcc gttcgtgcga aggccttcat
     3121 gagcgccggt atcaacgccc aggccgcccc cgcagcggcg ccgacgatcg aaagcaggac
     3181 aatcatgatc aaaggtgatt tcacgtcttt gaaaaggaaa agagcacccc atgtagaaac
     3241 catagcgccc atgtaaagct gcccttcgcc gcctatgttc cagatcttca tcctgtaggc
     3301 aaaggatagg ccgagcgcca caaaggccaa gggtaccatc ctcatgaatg tgctgaacag
     3361 tccatacctg ttcgcgaaag gccacgtaag catctctttg taagcctcta cagtttccct
     3421 gaaggccgat gaaagatttc ccaggcggcc cacgaagaaa accaggagta taattcccat
     3481 aatcagcagg gctaccgcca ctgatataac cggcacgata atcccggcgt atcggggtat
     3541 ctcgagtctc ttctctattc tgatcttcat ctcagtgatc ctcctgcctt gcgccggcca
     3601 tcatgaagcc tatctcttcg atatatgaag ggtcaggcgg agtgtagccc ataatctgtc
     3661 cttcatagat caccgccacc ctatcggaaa gtctgaagat ctcgtacagc tctccggcta
     3721 gaagtagtat ggccgctccc ctttccttct gcgagagaag ggacttgtaa acgaactcca
     3781 tcgcgccgat atccagacct ctggtggggt gttctgcgat tatgactttt ggttcgttcg
     3841 agagctctct ggccagaaca accttctgca aattcccgcc ggaaagatac ctgacctcgg
     3901 ttttagccga gggcgtcgat atagagaact cgccgatcaa ttcgtcggcc ttcgagtgca
     3961 tccgcctgcc gtcgagtttc cacggggagc aatgtaccag ttcgcagatc gagttcttca
     4021 tgaatagatt gctcatcacc gacaggttag gacatgtcgc catggttttc ctgtcctggg
     4081 ggataaaact gaggccggct ctgatccttt cggccacgtt accctgtctc aactcgtgcc
     4141 cgtcgagttc tatccttccg gaaactttct ttctcagacc gtagatggct tcggccaatt
     4201 ctcgctgacc gttgccggca acaccggcta ttccgagaat ctcacccgat ctgatctcca
     4261 ggctgagatc cctgaccgac atgagatctt tgtcgttttt gacgttgaga ttctctatct
     4321 tgagaatagc tctaccagtt tccaccaccg gtttctcctg gtcgaggagc acatctctgc
     4381 cgaccatcat tctaaccaga ccgcgccggt ctatcttttc tctctcgacc gtaccgacaa
     4441 ctttaccgcc acgcaagacg gttatcctgt cgcttatcgc gagcacttcg tctagcttgt
     4501 ggcttatgaa aatcaccgag gaaccttcgc tgactagctt ccttatggct ttgaaaagca
     4561 tatcgctctc ctgtggggtt agaacggccg tcggctcgtc gagtatgatt atgccggcac
     4621 ccgtgtacag cagcttcagg atttcgattc gctgcctctc cccgaccgaa agagtccaga
     4681 cccgcgcgcg tggattcacc ggaagtccgt aggcctccga aagttccttt attttcgatt
     4741 ctaccgtttt cgtctttatg acaaacccga aatccttcaa acctagaacc acgttttcgg
     4801 ccaccgtgaa cgacggcacc agggtgaagt gctgttgaac catgccgata ccgtatttga
     4861 gcgcgtgact gggcgaggtt attgatactt tgccgttgtt tatgaaaact tcgccccggt
     4921 cggcgtgata gattccgaaa agaatattca tcaaggtagt cttgccggcg ccgttttctc
     4981 ccaggagagc gtgtacctct cccttcttca actgcagaga tatgtcgtcg ttggccatga
     5041 cacccggaaa ggatttggaa atatgtttca tctcaagaac gggaaagtct tccaaagaaa
     5101 tcactccagc aagaggaaat aagggggcct gacggccccc gggagatcac tgtatcttac
     5161 ctactacgtt gtttatgaac cagtccatgc tgagaagatc gccgtcggaa ggtatttcgc
     5221 cttcggcgta cttcagattg ccggcctggt cgtagatagg tccgacgaag ggatgcatat
     5281 cgcctttgac gattgcattt ctcatggaca tcacgagctt ggcaacaccg ctgggaacaa
     5341 ggtcgctgag aacgatatcg acggctccgt catttatacc gccccagtac tgcgcgttgg
     5401 tccaggtacc atccatgacg cttctcacga ttttttcgta gtagggaccc cagttccaga
     5461 ccggtgccgc gaggaaggct ttcggagcga aggcgctcat gtcgctgttg taaccgatcg
     5521 aataaacgcc cctgtcctcg gccgcctgtt gcggtgccgg agaatcctga tgctgtgcga
     5581 tcacgtcggc ccctatatcg agcagcgata ccgcggcttc cttttctctt gccggatcga
     5641 accaggtgtt ggaccagacc acgtgtacct tcgcctgtgg atttacgtac tgaacaccca
     5701 tcgcgaaggc gtttattccc cttataactt cgggaatcgg gaaggcggcg acataaccga
     5761 ttatgttggt cttggtcatc gcaccggcca caagaccgct gaggaacctc ggttcgtaca
     5821 ttctcccgaa gtaggtaccc aggttatctg taacctggta accggaacaa tgcatgaaag
     5881 tggttttcgg gaaggacttg gagaccttca ccatgctgtc catgtagccg aaactggtcc
     5941 cgaaaacgag atcgtaacct ctttgagcgt agcctctgaa aacgctttca ccctcggcac
     6001 cctctggtac gttttcaatg aagtcagtga caatatcgct accgaagact ttctccagat
     6061 acagtctgcc ctggttgtgc atgaagctcc agccaccatc gccgatcggc gcaacgtaga
     6121 tgaaggcaac cctgagcgga gcggccagca tcgcggtagt aactaacaag agcgtgagaa
     6181 tgagcaggaa tcttttcact gtcttacccc ctttgcgaga agttggttct tccgacgtaa
     6241 gaattatacc cttacactat cgcttatcca aaatttgaaa gagcgtctct tcaacaaaaa
     6301 acacctccac ccgctgcgct caagacatac gagcggaggt acagaaatat tatactaccg
     6361 cttgtttaac agtcatatcc attctttgac tggcattgta ccggtggtaa aatattctgg
     6421 aagaacacac cacaataact aaggaggtcc agtctatgat aacgacgaga atggagaagg
     6481 aactgaacgc acagataaag gccgagtacg aatcggcctt catctatatg gccatggcgg
     6541 tctgggctca caagaacgcg tatttcggaa cggcgaattt catgtggaag caggccaagg
     6601 aagaggaaga acacgcaatg aaatttgttg ggtacctaaa ggatgtggat ggaaccatag
     6661 agatacccgg gctcgaaaag cccgaaggag agttcaaatc tctgctggat attttcgaga
     6721 agggcctcga acacgagaag tacatcacat cgaggattca caacctcgtg accgtttccg
     6781 acgaagaggg cgattacgcc actaacgatt tcctgcagtg gtacgttaca gaacaggtcg
     6841 aggaagagag aaacttcagg gatatcgtca aactgctcaa aaaagtgggc gatcacacca
     6901 acggaataat gatgatcgac gccaaattgg gagagagata aaaaataaac accgggggat
     6961 gtcgtacggg cgacatcccc gttttttgca gagcgagcga tcaacgagtt caactgagag
     7021 gagggggtaa caatgcgcaa gctttttctg gcgatgtttg tggtgatatc tttagccgtt
     7081 ctggtcttcg ctacgcaagt aaatctatct ataattcata ccagtgatat ccacggtaat
     7141 atcttcccgg tggattacgc gaccggtaag ttttcggctg tcggtctggc gaagatatcg
     7201 gcgtttctga gcgaaatgag aaagagcaat cccaacacac tcgccataga tacgggagat
     7261 ctgattcagg gtacgccgct ggcatattac tatgcgaaag tggataactc tggcgaaaat
     7321 cccatgatag cggcattgaa ccatatgggt ttcatcgcaa gcgtgatagg aaatcacgaa
     7381 ttcaactacg gtccctctat actcgaaagg gccatcggtg aagccggttt cccgatgttg
     7441 agcgccaaca tagtcttcga aagcagcggt gaggcggctt tcgcaccata cgtaatcgtg
     7501 gaagtgccct ttgaaggcga cacaatcagg gtcggggttc tcggtcttac gacgagcttc
     7561 atccccaact gggaggaacc caaacacata gccgggctga acttcctgga tcctgtgtca
     7621 accgctttag aatacgttcc ccagctcagg gaaaaggtag atgttctggt ggttgcctat
     7681 cacggcggct tcgagagaga tctgaagacc ggtgcgccga ccgaggaatt gcttggagaa
     7741 aacgtcggtt accagattct caaggaagtg aaaggcatcg atgtacttct caccggccac
     7801 cagcacagga ctatcgccga ggttatagac ggcactgtga tctctcagcc ttccaactgg
     7861 ggaaaggtcg caggcaagat agatctcgta ctcgaaaaga agaatggaaa atgggaaata
     7921 gtgaatagaa acattgccca aatttctatg gccaactacg atgccgactc cgaggtaatg
     7981 gcgatgttcc agaatttcga agaccgcgta cagaaatggc tcgaccagcc agtaggtata
     8041 gcgataggcg acttctacat cgacgacccg ttcgtcgcca gactggccga taactctttg
     8101 atcgagttcg tgaacagggt ccagagcttc gtaacggggg cgccgatatc ttccgttgcg
     8161 ctcttcacta acgatataaa gggatggaag accggaccta taacgataag ggatataaac
     8221 gccgtttata tctatccgaa cactttaaag gtgataaagg tcaccggcgc agatatcaag
     8281 gccgctctcg aaaagagcgc cgagtacttc acctatgaag acggcgagat agtggtcaca
     8341 gacagctgga tcaatcccaa gccgctccat tacaactacg acatttggga aggcatagag
     8401 tatatagtag ccgcagacag gcccgtcgga gagagagtat tgagcgtccg ttacatggga
     8461 agacctctgg atatggacgg cgaatacgaa gtggtcctga acaactaccg tgccggtggc
     8521 ggtggcggat acactatgtt ccagggcaaa ccggtcgtga gagaggtaat gctcgaagtc
     8581 tccgagataa tcgccgatta cgtacttgaa agagttgccg ttagcgcaac tctcgatcgc
     8641 aactggtcgg ttggaaccgg tctcatccac acggtcggct ggaatgaaac gctaagaacg
     8701 atctcggaaa tgtacggaat agacgcctcc gagatcatga aatacaaccc cgatttgaaa
     8761 agggtcgaga ggatccccgc cggtactaag atcattatct atagacccac gcttgaagaa
     8821 gtgccggtga tatgagctct caaagataaa aaagggcgga gagacccgcc cttttttata
     8881 tctcagctaa ttgaggaatg gcaaaggccg gcaaaattta tccactagtt ttcatgagtt
     8941 cgagatcctt ggaaaaaacg gccggaagga ttatcacctt acagcctgga tatgtttttt
     9001 gatgcttttc ctgaaccgtg ataacccctg attttgtaag tcagaatctt caagcgtctt
     9061 tcccggtcgg cgtagctacc ttcattcgaa gtattttctc ggtccatgac tctcgacttt
     9121 tttcaaaaag cttctaacgg tctccatgta ttcttcgggc ttctccaggt ggtgttcgtg
     9181 ggaagcgtct tcgataattt tcatttcaca gttgactatc tccctacggt aaagctctat
     9241 cgtcgagggc gtcgcctcgt cgaatctgcc acatgtcagg agaaccggaa cctctatctc
     9301 ccccagtcta tgaacacaac tgtaatttct aagcgtgccc gtgaccgtga actcgctcgg
     9361 tccgtacatg tattcataaa cggccgtgcc catcccttcg agagtcctgt tcaagcaatc
     9421 cggccacgga tcgagacggc agacatggcg gttgtaaaag gtcctcatcg catcggcgta
     9481 ttcccgagag tcgaagcgac cgcttgcttc ggctctgtgt atggcgtttt gcctttcttt
     9541 aggcaggaag gcaagcagtt ttctaacgtc cttttcgaat tgtggaatgc tcatagccgg
     9601 tccggatagt accatgctga ctacctcttc aggtttgaag aagtagtatt cgcaggccag
     9661 gatagttccc caggactgcc ccaggatgtg tactcttcca agtctaagac gttccttaag
     9721 aagattcaat tcgccgacga aatgttccac ggtccagaga cttttatcgg aaggcctgtc
     9781 ggaccttcca caacccagtt gatcgtagaa aataaccggc ctttcgtcag acaaagccga
     9841 aagcggttcg aggtaatcgt gaggggtgcc ggggcctcca tgtagaatta tcagcgggat
     9901 tccgctctta tccgccccct cgacgcagta ccagatcttt ccgcctttta cttcgacaaa
     9961 accttcggct ctcttcccgt tttcacccat cgatattacc tcccttccca atcattacga
    10021 ttatacaacc gatcaacgtg acggtcggct tgacggccgg tggtcagaaa gtctataatc
    10081 gattagacag ctgtctaatt gaaaggaaga gatcccgatg gttgattgtt gctatgaaca
    10141 tgaaaataaa acagatacgg cttttttaag gcttaaggca agtgaagtct ccgggctttc
    10201 aaagctgttc agggtcctgg gagacgaaac aagaaccagg atcgtgtatc tcttgtcgat
    10261 caccgagctc tgcacctgtg acctggccag ggtactcaat cttacgttgc ccacgatctc
    10321 ccatcatctt aagcttttga aggaattgag gctggtcagg agcagaagag aaggaaaagc
    10381 cgtttactac gcgctggccg acgaacatat acttgtactc gtcaagacgg cgagagagca
    10441 tttcgaagag gagtcgcatt gaagacaaaa cacagaatca agggacttga ctgtcctgtc
    10501 tgcgctgcca aaatcgagga caggataaag aagatgggtt acagtgcgaa gatagatctt
    10561 gcctcgcaat cgctgataat cgaagatggt gacatagaag agataaacag aatcatatct
    10621 agtgtcgaac cgggtgccgg aatcaccgtg gattcaggcg gttccactgt tacgagaaaa
    10681 ttcgggattc tgctcctatc ttgcgtcgtc tatctcttcg cactgatcac cggccgatac
    10741 cttcacggaa ggtacgaacc gctcgagtac ggaatataca tactggccta tatactggct
    10801 ggaaacggtg tgcttctgaa agccgggaga aatattcttc atggaaggct cttcgatgaa
    10861 aactttctca tgaccctggc caccgttggg gcctttctga tccacgagct gccggaggcc
    10921 gtggctgtga tgcttttcta caacgccgga gagattcttg aaggcatggc gctcgccaga
    10981 tcgagaaaat ctatttcctc actggtcgat ctgaaggccg agacggtgaa cagagttacg
    11041 ccagaggggt tagcggcagt tcctgcctcg tctatggtcg ccggagacag gttcgtggtc
    11101 aaaccgggtg agaggatccc cctggacggc tcgatcgctt ccggtgagac ctggctagac
    11161 ctttcgcttc taacgggcga gagcagaccg gtgaaggccg gcgaaggtta cggagtcgtc
    11221 gccgggacga tcaatagtac cggcctgata gaggtagagg ccacggccag cctagagcga
    11281 tcatacacca gcagaatgct ccagatggtc gaagaggcct cttccagaaa atccaggact
    11341 gaacgcttca tcacgaaatt ctcgcgtatc tacacgccca tagtagtttt gatcgccgcc
    11401 tttcttgcca cggtaccgcc cctttttacc ggaggaaatt acggcgaatg gttccacaga
    11461 gctcttattc tgttagtcat ttcctgtccc tgcgcccttg tgatatcagt gccgctcggg
    11521 tatttcgccg gtatcggaaa ggcttccaga tacggtgtgc tggtcaaagg gagcaactat
    11581 ctcgaagcac tggccgggat agatacggtc cttttcgaca agacgggcac gctcacagag
    11641 ggaaggttcg ggataagcga aatctccggc cacaacggaa tggcgccggc ggaagcgatg
    11701 ttgatcgccg cgagtgtgga gagcaactcg aaccacccta tcgccagatc gattgtcgat
    11761 cacgccttct cgatcggttt caaaaaagaa tatcttctcg ccgttagcga ctacagggag
    11821 ataccgggtg aaggagttca cgcgaccgtc gaaggcaagg agatagcgct gggcaatgaa
    11881 agaatactgc gaggttcgac cattctcccg gctgcgaagg gtaagtcgaa gatctatctt
    11941 acagttggcg gaaagctgtc gggaacgatc gatatctccg atagattgaa agaagattca
    12001 aaaatggcga tcgaatctct ccgcgaccag ggcctggcaa gcatcgcgat gctcaccgga
    12061 gattcccccg atgtggccgc agccatttcg ggcgagcttg gcctggactg gtacgagggc
    12121 ggactgaacc ctgaagggaa gctcgaaacg ctggagagat cttcccggac gggctgtaag
    12181 gtagcgttcg tgggtgacgg tataaacgac gcgccggtga tcgccagatc cgacgtcggc
    12241 atagcgatgg gaaggattgg ctccgatgca gtgatagaaa cggccgatat agtgatatcc
    12301 gatgattctc cattgcaggt atcgagagcg atagcgattg ccagaagaac acggagtata
    12361 atacttcaga acatatgtct ggccatagga acaaaggcgc tcttcatagt tcttggggct
    12421 ttcgggctgg tcagcatgtg gggagcggtt tttgcagatg ttggtgtggc gttactcgca
    12481 gttttgaatt ctctgagggt ctttcgcgat tgattctccg gagaccggtg gttcgatgaa
    12541 gccagaaaaa ctgtattacc tgtacagcgg ttccgtctat tttctcttca atctagtatt
    12601 caccatcaac ggcatctact tcgtcgaatc ggtagggctg aatcctctcc agttggttct
    12661 cgttggaacc gtggtcgaaa cctcggcact cctcttcgaa atacccacgg gtgtcttcgc
    12721 cgatatgtac ggcaggagaa gatcgatcat cacaggacta accgtgctgg gtgtcagctt
    12781 catcctcgag gggagcctgc cctttttctg gaccatcctt ctggcacaga taatcgccgg
    12841 cgccggatat acattcctga gtggcgccga tatagcctgg gtcgccgatg agagcggaat
    12901 tgagaacctt gactccgtgc ttgtgaaagg cgctcagttc gaacagatcg gctcgctcgc
    12961 gggaatatgg gccagcgttc tcctcggact gtttgctttg aacttcccga tagttctttc
    13021 gggagtttcc atgttcctcc tcgccctgat cctgagacga ataatgaagg agaacaagtt
    13081 cagacctaag caccggggca aacccctgaa agactttctt tccactctcg cagacggcct
    13141 tggacagata aggacttcag cgagcctgac gataatcctc ttcatcagtt tggggatagg
    13201 tctctacagc gaagggttcg acagactctg gatactgaag tttctcgaag atctgaagat
    13261 aaccgaggaa tttggattac gttacgtgct gtggttcggg gtgataaact ccggtgccat
    13321 ggttttgagc atcgccgttc tatctttcct taaaagactg tcttcccgta actcttcaaa
    13381 gctcctgcta tcctacctgg tcatcctgaa caccatcctg gccgtggctt tcatcggatt
    13441 cgccctcagc ggcaacttcc cgctggccct ggccttctac tgggcgggtt tcgtggccag
    13501 gcggaacgat tatcctctct acgcaagctt tatcaacaga caaatagagg tttccggcgt
    13561 gagggcgacg gtaatctctg tggccagaca gttcgattcg ctcggccaga taatcggagg
    13621 cccgataatc ggcctgatag cccttagttc aactaccctg gccctgggag taacgggatt
    13681 gctggttttt cccgtagtgc tgctatacat ctggctcctc cgaagagaga gccgtccaga
    13741 ccgaaccggg aagtaaattg ctcgcgggaa gaaccgtcct tggtccttcc ccggatcatg
    13801 gacccgtctt tcgaaagaga aaaagaacct actcaaccat tattagaaag caaatttacc
    13861 gccggaagtt actggcattg cgaatgcaat ctttctcttt catgggtctt tttcaaacta
    13921 tacacagata aattagaaat atttggctct taaaaaataa atatgaatat gagcattgac
    13981 aaattgaatt ttataaagta tattatcaat gattagaata acaaacaccg aaaaaggaga
    14041 tgattattat gaaaaagaag agttattccc gtaattacga gagtttagat aaaatcactt
    14101 tcgagattca ttcgaagatg tacagaatta ctatgggtat atgaggtaga tcgatgaaga
    14161 agacactggt tttaattcca ctggtttttg tgctgttatt tctttcgacg ggctgctcgc
    14221 tgatcttcac gacgagaatc aacagtcagt acgagaagga aatcgatatt cttgaaacga
    14281 aggccctcaa agtcaccggt acaaacggtt cgctcaccgt tctggtctgg gagaaggact
    14341 acgtaagagt gacgggaacg aagttcgtgt caggattgaa cagttcgcag gtaaaggcct
    14401 tcaacaactc tcttgacgtt gtctggagca atctgggaga gaccctcgag atagggctca
    14461 gtagaaccga tcgacccggc ggaataaatt acggagtttc aatgaccgtt tatgttccga
    14521 gggaactgta tgaatctcac ttttataaga catctaacgg ttcggtgagt atttcgggga
    14581 tatccgggga gtgcgtcata gagtctagca acggcaacat aagcattacc ggcttcgagg
    14641 gcagcatcta ttcggtgact tcgaacggcg gttataagat caacctttgc aagctttacg
    14701 gagacaatta ttttaagacg agcaacggat cggtagtcgc caagttgctc acgtctccga
    14761 ccggaagact ggagatctac acaagcaacg gaaagataga tttcaagctt ccctggaccg
    14821 caggagcctt tttgactgct gctacatcca acggaaatgt tgatgttcag ggcttcagat
    14881 cgatctcttt cgaaaagatc gacaagaacg atctgagagc cgaaataaac aactggaggg
    14941 atcttgatct ttctctcaga acatccaacg gagatatcac cctaaacgga atgcaataac
    15001 tttcttgaac accccggaca cttcaaagta agacctttct tctacgggcc ccgtgagggg
    15061 ccttctcgtt gttctctcag agaacctatt accagtttgg atcgtctatt ctctgaagca
    15121 aaaagccggg gggtgtttct cttgagaaaa gttatgttga ccgtggcaat tctggtctta
    15181 ttcgtttcgt ttacggtggc acttgttggc ggctcgcagg tgccggagtt tttctcggac
    15241 tggcgtttcc tgcgcgctca tgtcttttcc gactatgtgg tgtacgaact catgcaaact
    15301 gatatgtcag gctttgaggt gaaagtcgtc caggccggtc ccaccggtgg gctcaaaatc
    15361 gggttctttt ccaaatcggt cttggtcgca gagagcggcc cgaacttctt catgggtctg
    15421 tggaacgatg tcttgatggt agacacggga acggcccccg aacccagact cctctctctg
    15481 taccatatac cggaaaagcg gctcgttcac cgcgactcgt atttctcacc ggtagaaatc
    15541 gatggagaac ggctgcttta ttacagggat atcggattga ggttttacga tcccgtcaat
    15601 gagaaaagac tccccgagga gctggcgttt ctcgaagaga gcctagtgtc catgctcaaa
    15661 gaccagcctt atctgggtgt ggccatctcc gagaaggtat atctggatct cctgacgttc
    15721 gaactggtga acacgggcga atacgttatt cgttacgtcc agtgacccgg gtctctccga
    15781 ttatcctgga tgcggtaagc caggcgtttc tctcctcgcc tatgggcgag gtgttcctgt
    15841 agtcgttttt accgatgtac ctttcgagca tttcgatcac ggattcgatc ttgcttttct
    15901 gaagagtcac gaaccattcg accgtagccg ctctcatgga agcggcaacc tctctcgcgt
    15961 ggtctatgca cagaatggag gaggactctt catacagctt tctgattttc tccgaatcga
    16021 agaccttcgc tatggctatc acgtacctct tttcgctctc cctggacact tcgcagagat
    16081 ggcagccacc ggtgtttctg gatgatgaaa ctcttttgaa gagctttctt tcgcccggac
    16141 caatcaggtt gagctgtccg aaaagcagct ctttgaagac aaccgagacc ggaagactgc
    16201 cgatatcgga tttctctctg gccatttcca cgatccgtct ggagtgctct cgacacagcc
    16261 ctcccgattt caacctgaac ctcgcatctt cgttgtgaag cagattgtag aag
//
LOCUS       DAOOLY010000019        15930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3878_length_15930_cov_4.874583, whole genome shotgun sequence.
ACCESSION   DAOOLY010000019 DAOOLY010000000
VERSION     DAOOLY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_15930_cov_4.874583"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="ssrA"
                     /locus_tag="PLD15_01930"
     tmRNA           <1..344
                     /gene="ssrA"
                     /locus_tag="PLD15_01930"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            816..2042
                     /locus_tag="PLD15_01935"
     CDS             816..2042
                     /locus_tag="PLD15_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPB62687.1"
                     /translation="MMRDLKRWATIVCVVLILASPAFSVMNFHPVIQEFQEIIEEDPV
                     LFMLFTQMFEEIPSSPEFEMDPAGNFQIKDYRQMLQVMNEVLTQAPEFNKTDLVGFPI
                     NAVINWAMGTPAGTTAFLNEKVNRQLKKILNQWAVFLGSTDSRYVLSGDPEKGWFGRD
                     AMEAMPNFVEDFQCDPNLPHYGFASWDDFFTRQFREGKRPVEYPDDNAVITNACESAP
                     YRIATNVQIHDRFWIKEQPYSLSHMLAGDPTVPLFEGGTVYQAFLSALSYHRWHSPVS
                     GRIVKTRLIDGSYYASALSAGLDPASPNESQAYITQVASRALIFIEADNPDIGLMCVV
                     FVGMAEVSSNEITVYEGQHVKKGDQLGMFHFGGSTHVLIFRPEVNLEFDLRGQTPGPD
                     SVNIPVRSKIATIQGE"
     gene            complement(2185..2559)
                     /locus_tag="PLD15_01940"
     CDS             complement(2185..2559)
                     /locus_tag="PLD15_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62688.1"
                     /translation="MGNIDVIMYGKGKKSVNIAEIFQSCPYTVVCTACRGYVWSHFRL
                     PENHLWWLEATEKDPIDTVGLESVELFFPEKLKGESEFSRGETSPMLEISPCKSYCPE
                     CTLYRTRCEGCPATVHCLLERF"
     gene            complement(2660..3220)
                     /locus_tag="PLD15_01945"
     CDS             complement(2660..3220)
                     /locus_tag="PLD15_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HPB62689.1"
                     /translation="MKRAMNVFLVLIIFIVTATASFTMSEEDGIAYMREEEKLAQDVY
                     NVLYELWGLKVFKNIAQAEARNTDAVLSLIEEFGYEDPAGQNGVGVFKNKELQALYDY
                     LVEMGSESLLGAVKVGLLVEETDIKDLEELLEVELNARTERVYRNLLAGSENHLRAFY
                     RQLEKLGGDYNWSVLDFERALEILSK"
     gene            complement(3355..4737)
                     /locus_tag="PLD15_01950"
     CDS             complement(3355..4737)
                     /locus_tag="PLD15_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPB62690.1"
                     /translation="MKPGCYIDRGPCDWQILQQEKGSAEVGLAGSCLPSGEIPDRQRV
                     FVRVVREDSSETVLPWLQATLHKGKRWEVSLELPIGGLYRIETSLKPDSLGIEYAFRG
                     DTVHHVGVGELFAITGQSNSAGYGKDPIFDPPELGVHLLRNNGKWDLASHPLNDSTGS
                     IHEVNAETINPGHSPYLSFARYLKRELNRPVGLIQCSLGGSSLGQWNPAESGELYRNL
                     LEIVANCGKLRGMLWYQGCSDTTIALSQTYGERFANFVETLRQDLAAPDLPFFTVQLN
                     RFVADPGPWGMGDEGWSKVREAQRRAVKELPGVFVVPAIDLPLSDNIHNSSYGNLMLG
                     ERLAKSVLKHLFARDLNADAPDIAAALATGGRKLRLVFDNVSSRLYAFDLKPEDLPLS
                     ISDESGEIEIKDYTIEGNEMLLRLSREPGVYGVVHYQHGANPGCFSIIDAISHLPALA
                     FYGFKISRDL"
     gene            complement(4734..4979)
                     /locus_tag="PLD15_01955"
     CDS             complement(4734..4979)
                     /locus_tag="PLD15_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62691.1"
                     /translation="MQTHPLLLLGLSHPDLVVFGGKISGAFYYIAPALKEELEKAAKV
                     GRFAAFPGVLKTIDPERCTLSWLALYFVERCMRGVSV"
     gene            5263..6495
                     /locus_tag="PLD15_01960"
     CDS             5263..6495
                     /locus_tag="PLD15_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB62692.1"
                     /translation="MTSRERIIEALNHREADRVPVDLGGMRSTGIHAMAYRRLKEYMG
                     LPDRPVKIFDVFQQLALVEEEVRRRVHSDVVELKRLDGGFNTRIDKWKEYDMFEDGGR
                     YLLPDGFDSVTMPDGSRAIVREGRIVAIMPKGGFYFDGKHFPLADTHDRKAIDEAVRP
                     GISDGEIAFLKDQLRELKSSTDCAVMGTFGGNFLEAGHSYFGYQEFMERMITDRPLVE
                     YFLDRLLEKYTIELERYLFEVGGDIDLIQIGDDYGTQENTQISPRIFRSLFKPRLKAL
                     CDFIHSKRPGLFIFLHSCGSVYPFIGDFIEAGVQVLNPVQTNAKNMDPAILKREFGGE
                     IVLWGGGCDTQHLLLFGTLEEIEEDVRRRIDILAPGGGFVFASIHNIQMEIPPEKILR
                     LFDTAYEYGRGAYSDRKR"
     gene            6709..7710
                     /locus_tag="PLD15_01965"
     CDS             6709..7710
                     /locus_tag="PLD15_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HPB62693.1"
                     /translation="MGRTRSITLAILFLLIVSAAMALIELKDFSLASIDMKEFQAVQP
                     MYTISALSRFNGRSGSSAYVSVEGAIYDFSQLRRWIGGNHMSQHSAGTELTYELKSLS
                     PHREGKIAGIEPVGVLVVTLEELKNFDGKSGKKGYVAAWGKVYDVSRLGRWRNGSHQG
                     GMHLAGEELTSELTKDSPHGVRKIEGVDAVAILGVTIDELKTMNGNGGKKAYVAVEGK
                     VYDVSGLASWKGGSHQSGLHLAGNELTVEILRDSPHGATSLDKAYLIGILVFTPEQLK
                     RYDGVVESRKFVSYEGVVYDVAGIDWTAGYGIELGASLGSELSKLEDAHIIGYVVRN"
     gene            7885..8514
                     /locus_tag="PLD15_01970"
     CDS             7885..8514
                     /locus_tag="PLD15_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPB62694.1"
                     /translation="MFQRLIEGRLLIVIRGLDTGRSIDLCGLLYDSGVFFAEVSFSDR
                     WADESLKELKKHFGDKLLLGAGTIFNADDYSRALRAGADYILSPGFSHEVAALSLKDG
                     IPYIPGVYTSTEIQMALNMGFTILKLFPGELEGLRLLKAYRGPFPQARFMPFGGVTPE
                     NAAAFIEEGAAALGVGSYIASPELLQERKYGEISERISRMKIAAGIIDD"
     gene            8507..9409
                     /locus_tag="PLD15_01975"
     CDS             8507..9409
                     /locus_tag="PLD15_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB62695.1"
                     /translation="MTDLAIDVGGTKTLVVVFEDDQVKPLYVERFPTDSTAGPENFIA
                     RLAKVCSKYVDPPDVVHWGLCTAGAVDVSTGRLIWSPNLGWKDVNLHERLSQLFGDRG
                     IVENDCNAAAYGEWSTRPGIDSLVYVTVSTGIGMGIVSSGHIMRGSHGFAGEIGHTTV
                     LPDGPECACGRRGCLQAVAGGRGLSIQVERFLGKRLATEEIVDLARENKPEYVEIVVG
                     GAAILGRFLANMIDTLDPAVLVMGGSLGKNPHYRRLVVDFFTKFYYRLPDKTVGIEPP
                     SAEPDPCIIGVLQLSRSMGVRKSG"
     gene            9402..9755
                     /locus_tag="PLD15_01980"
     CDS             9402..9755
                     /locus_tag="PLD15_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB62696.1"
                     /translation="MDKAIKYSIHEGDFSRGMEGFSSKAVKIVGPFSGRPEAHRDQHD
                     LYVVISGKARVKTGKLTGDIQEIADGEYRSDEMLASEEFTLEEGESLLIPASVAHTVL
                     VDSGTYVQWVFKIDA"
     gene            complement(9771..11627)
                     /locus_tag="PLD15_01985"
     CDS             complement(9771..11627)
                     /locus_tag="PLD15_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPB62697.1"
                     /translation="MNWFGAAYYPEHWSRERWKEDARVMAYLGINVVRIGEFSWGVVE
                     KRPGEVDFTLLDEAIEALYSEGIRIIMGTPTAAPPSWLLKKYPEVLRVAGDGRKPGTA
                     GRRQYCFNSPRYREETERIVGIYVDHYGKDPRIIAWQADNEYGCHGSALCYCNNCAGA
                     FREWLREKYGTLDNLNQAWGMVFWSNICSDWEEINPPKPGPVSYNPSMVLDYRRFSSD
                     SVIEYHDIHKEIIKKNSKAPLTHNLMVNFTDIDYRKLAKRIDFVSWDNYVAGEYDPDL
                     QALNHDLMRSLKKAPFLVMEQQPGRVNWRPVNNTYPSGQLAFWIKQSVAHGAFGSLVF
                     RYRQLPFGAEQFHSGLLNYDGTMTERARVFAETIEELRNWKIEDPEKEAAIYIDYENF
                     WIGETDNLNGKFRLLFDSILPIYKAFRSFGYNIDFLFPGESPQGYSFVAVPSAFKIEE
                     DFVRELLNFEGKIFITAMTAQKDKNNNIRMQKADDFQLLTGIRVVDFGGFEGPLKVSS
                     GKSVCDCGFVAEEIETLDDCQPVARFIDGPFRGKPAVTVKGNCCYVSTIPDVELIKKL
                     FLEASIERKAEGSARLLSMKSYRVILNPYPHSIRLKIGGETHSLQEYEVRRI"
     gene            complement(11781..13505)
                     /locus_tag="PLD15_01990"
     CDS             complement(11781..13505)
                     /locus_tag="PLD15_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB62698.1"
                     /translation="MILYTCSYIPLEILMGTGKDFERLLSAASSSCHELGCNLCGYAK
                     IVYGAGMELDKGDLLLVADSCDAMRRVGDLLSESSRAGVFMMRLPWKRDERAVEFMAS
                     EILRLIEFLEEKGIDVNLSAGVERFNGLIDHVRSLESTLVGRDLTELYLSALNGLKHD
                     PTGSRVKNKTAGKDFGLAGGIVDMSEIDAIIESAGGVITRNDTCLGARPFASRTRVGE
                     DPAFYVAQRLIGWRAPCGRFSEESKDECEGLDGLLYAIPKFCDFYDFTRNTSNSKIYR
                     AEIDYPLNSHGQIATRVGALLEKNLARRSEIERGERSVFLGIDSGSTTTNGVLTDSTG
                     KILRWKTIRTGINALASARRLYEELLSQSGGSKEEIARIVATGYGRELIDFADETVTE
                     ISCHARAVARLFPSAIGIVDIGGQDSKVIKLSAEGNVEDFSMNDRCAAGTGRFLEVMA
                     KVLELDTGEMASLALRSSRELSISSVCTVFAESEVVSLIGRGEKIEDISAGLFNAIVK
                     RVAAMYVRLGSPEPLVFTGGVARNAGVVRSMERVLKRKITVPEVPDIMGAYGAALFAM
                     DIPGEKGQ"
     gene            complement(13502..14758)
                     /locus_tag="PLD15_01995"
     CDS             complement(13502..14758)
                     /locus_tag="PLD15_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPB62699.1"
                     /translation="MSKKSVGFGRFLRRYINDEMKFRKWLNLGIAFENFRRRYLPSRK
                     ARFLRIVNSLAVEEVYYSLTGRETAWVNLLAPSELLLPLGINPVSAEGVSGMLASMHL
                     EGFAISSAAESGIPDSLCTFHRASLGASIMKLFPAPRFVMTSTIFCDGNLPTFKRISR
                     EYRVPFILIDVPRGRMKSSVGYVHSQLKEAIGLIEEITGKPYPMNLLSDTLAIERQTV
                     ENLTRVRKEMKSSHLPQELYEYMNSLYVMHTLAGDARLKRASEEFGRNLITDRSEQKK
                     ILWLHIPPYYDNELFKIFSPQSKHTVVGNELMWDWLYPVEPSRPLESLAEKLVFNPLC
                     GGVEDRWKQCAFLADDFRADGIVHFTHWGCRQSAGNVSYLKKMFESKGIPFLELTGDC
                     VDHTSEGAGQLRTRAQAFLEILEGKR"
     gene            15034..>15930
                     /locus_tag="PLD15_02000"
     CDS             15034..>15930
                     /locus_tag="PLD15_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB62700.1"
                     /translation="MKKRVSVVLIALIAIPAFCFAAVAAPQIFTFAGHNDVMTLDVSQ
                     MNDEMSALVMYALNEALIRNSYNNIIPGLAGSFDVSEDGTVYTFHLRDAKWSDGVPVT
                     AKDFEYSFLRTLNPETGSSQVSEFDSVLNAKAYYSGEITDTSLVGIKAFDDRTLIITL
                     AYSDPFFISEMAEGINFYPIRKDYVEKYGMSYASSAQTFIGCGPFVLKEWVQGASITL
                     EKNPLYWNAGSVKLEKVIELIIPDENTRVGMYDLGEIDAIYSISKVQTIMHPEYGSRS
                     GGTLQYLAFNCSDGLVMSNMNLR"
BASE COUNT         3783 a         4088 c         4168 g         3891 t
ORIGIN      
        1 aaacggtttc gacggggtca acgcacctgc aggagcgagt cgaggttccg gagccgcgtt
       61 aaaaaaccgg acaaatagaa atgccaacaa tgaatttgct ctcgctgctt aataattagt
      121 aagtagccgc tctatcagct ttgtggttcg atgggctgag gtgagcgtca gaaaatcgaa
      181 cctaacctgt gccttcgctc cggaggttcg gggacattca ggagcttgga tggtcggact
      241 ctgccagtga agtcttgacc gttgacgacg atcgctggct gcgctcggag accctgcagg
      301 gaacttggtt tcggacgcga gttcgactct cgccgcctcc accatcgtaa ttacacgcgt
      361 catttagcca aagcgctgct ttgaaatggg tatggaattt tttgcttgcc cccacactgt
      421 ataaagtgtg ggggtttttt caagttgatc ctgattcagg aagtgtcaaa tacagataga
      481 aaaagcgtta ggtcataaga acgtcaggct aaggtcactt cataccagtt gaaagtgtat
      541 aaaaagctcg caaaaaggaa ttggcaggac ttagagcgat ctgttattcg ctgatatagt
      601 ctcgccagat aatagatctt ctcaccaccc aacgagtttt ctcgtgaatt tggccttcct
      661 gcaaggacag agttctataa tagatgaggc ggttgcctga aagtcatttt ttgatggaaa
      721 cattcagcac tgaagaacat aagtctttta tagaaaaatg caatcggtag tatcatcgga
      781 agaatcgctg gtatccaaag aggaggagtg tcctgatgat gagagatctg aaacggtggg
      841 caacgatagt ttgcgtggtt ctgattcttg catcgccggc tttttctgta atgaattttc
      901 atccggtgat tcaagagttt caagagataa ttgaagagga tccggtcctt ttcatgctct
      961 ttacccagat gttcgaagag attccttcgt caccggaatt tgaaatggac cccgcgggaa
     1021 atttccagat aaaagattac aggcaaatgc tgcaagtaat gaacgaagtt ctgactcaag
     1081 ccccggaatt caataaaacc gatttggttg gatttcccat caacgcggtg atcaattggg
     1141 caatgggcac tccagcagga accaccgctt ttttgaacga gaaagtaaac cggcagttga
     1201 agaaaatact caatcaatgg gccgtgtttc ttggctctac cgattctaga tatgtgctga
     1261 gcggggatcc cgagaaggga tggtttggac gggacgctat ggaagccatg cccaactttg
     1321 tagaagattt ccagtgtgat ccgaaccttc cccattacgg cttcgcttcc tgggacgact
     1381 tttttacccg tcaattccga gaaggcaagc gccctgtcga ataccccgat gataacgcgg
     1441 tgatcaccaa cgcctgtgaa tcggctcctt acaggatcgc gaccaacgtt caaatccacg
     1501 atcgcttctg gatcaaagaa caaccctatt cgctctccca catgctggct ggagatccta
     1561 cggtgccgct ctttgaaggc ggaacagtct atcaggcctt tctgagcgct ttgagctatc
     1621 accggtggca cagtccggtc agcgggcgga tcgtcaagac tcgcctgatc gatggttcgt
     1681 actacgcttc ggccctgtcg gcgggcttgg accccgcaag tcccaatgaa tctcaggcgt
     1741 acatcacgca ggtggcttcc agagctttaa ttttcatcga ggccgataat cccgatatcg
     1801 ggctgatgtg cgtcgtattt gtcggtatgg cagaagtctc ttctaatgaa atcacagtct
     1861 acgaaggcca gcacgtgaaa aagggcgacc agctgggtat gtttcacttc ggcggatcga
     1921 cgcatgtgct cattttcagg ccggaagtga atctcgaatt tgacttgcgc ggtcaaacgc
     1981 cgggaccgga ctcggtgaac attccggttc gatcaaagat cgcgacgatt caaggagagt
     2041 aatcaacgaa agtcttgaca gactccgttt tttgttgcat atcgaaaaat taattctacc
     2101 tgttatggat cacggacatg ataactgacg actatgagga attcctcata aaggcaacgg
     2161 tagttatcca gcatcaggag gagattaaaa ccgttcgaga aggcagtgaa cagttgcagg
     2221 gcagccttcg catctggttc tgtaaagggt acactctggg cagtagctct tgcagggcga
     2281 tatttcgagc atcggtgagg tttcgccccg ggagaattca ctctcgccct tcagtttttc
     2341 gggaaagaaa agctcgacag attctagtcc caccgtatcg atcgggtctt tttccgtcgc
     2401 ttccaaccac cagaggtgat tttctggcaa cctgaaatga gaccatacat atcccctgca
     2461 ggcggtgcaa actaccgtgt aagggcaact ctgaaagatc tctgcgatgt tgaccgactt
     2521 tttacccttt ccatacataa tcacatcgat atttcccaca tgactacctc cgtcgatatt
     2581 tatacagagt tattatcgca tctttcaacc aaaaaataac ggcgggaaac ccgccgccag
     2641 gtagtcagtt atctgatgct tatttcgaga gaatctccag ggctctttca aaatccagca
     2701 ccgaccagtt gtaatcgcca ccgagttttt cgagctgtct atagaaagcc cttaggtgat
     2761 tctccgagcc ggcgagaagg tttctgtaca ctctttcggt tctcgcgttg agttcaactt
     2821 cgagaagttc ttcaaggtcc tttatatcgg tctcttctac cagcagtcca actttcacag
     2881 cccccaggag agattcgctt cccatctcga caagatagtc gtacagagcc tgtaactcct
     2941 tgtttttgaa gacgccgact ccgttttggc ccgccggatc ttcgtagccg aactcttcga
     3001 ttagcgagag aacagcgtcg gtgtttctgg cttcggcctg ggctatgttc ttgaagacct
     3061 taagtcccca taattcatac agaacgttat atacatcttg cgccagcttt tcttcttctc
     3121 tcatatatgc tatcccatct tcttcactca tagtgaagct tgcagttgca gttactataa
     3181 atataatcag taccagaaat acattcattg ctcttttcat ttttgccacc tcctttgtat
     3241 gctttcatac aaatattagt ccttcaatct tagaggttgc tgagaagagt cttggaattt
     3301 catgagaaag gccggtcttt accgtgccgg ttttcttgca agtatcagtc gaagttacag
     3361 gtctctagat attttgaatc cgtagaaagc tagggccggt agatgactta tcgcatcaat
     3421 gatcgaaaaa cagccgggat tagcgccgtg ttgatagtgg acgactccat agacgccagg
     3481 ctctctcgag agacggagaa gcatctcgtt gccctcgata gtataatctt ttatttcgat
     3541 ctctccagat tcgtcggaaa tagagagcgg aaggtcttcg ggtttgaggt cgaaggcgta
     3601 cagtctggag gagacgttat cgaataccag acggagcttt cttccgccgg tggccagggc
     3661 agcggctatg tctggcgcgt cggcattgag atctcttgcg aagaggtgtt tgagaacgct
     3721 tttggcgagt cgctccccga gcatcaggtt gccgtaagaa ctgttgtgga tgttgtccga
     3781 aagaggcaga tctatcgccg gtacgacgaa gactcccgga agttccttca ccgcacgcct
     3841 ctgggcctcc cttactttcg accagccttc gtcgcccatt ccccatggcc cgggatcggc
     3901 cacgaaacgg ttgagctgca cagtgaagaa gggcaggtcc ggcgccgcca gatcctgtct
     3961 gagagtttcg acgaaattgg cgaacctttc gccatatgtt tgactcaaag caatagtggt
     4021 gtcggagcaa ccctggtacc agagcatacc cctgagtttt ccgcagtttg cgacgatctc
     4081 cagaagattt ctatatagct ctccgctttc ggccgggttc cactgaccga gactcgagcc
     4141 tcccagcgag cactggatca agccaactgg cctgttcagc tcccttttca ggtatctggc
     4201 aaagctcaga taaggcgaat ggccggggtt gatcgtctcc gcgttgacct cgtggatcga
     4261 tccggtggaa tcgttgagag gatggctcgc caggtcccac tttccgttgt ttctcagcag
     4321 atggacccct agctctggcg gatcgaaaat cgggtccttg ccgtagccgg ccgagttgct
     4381 ttgaccggtt atggcgaaaa gttcgcccac accgacgtgg tgtaccgtgt cgcccctgaa
     4441 ggcgtactcg ataccgagac tgtcgggttt cagagaggtc tcaatcctgt aaagcccgcc
     4501 gatcggaagt tcgagcgata cctcccatct tttgccttta tgcagcgtgg cctgaagcca
     4561 gggcagcacg gtctcggagc tgtcctcgcg caccactctc acgaagactc tctgccggtc
     4621 cggtatctcg cctgagggga gacaactccc cgcaagtccg acttccgcac tccctttttc
     4681 ctgctggagt atctgccagt cgcaaggtcc acgatcgata tagcagcccg gtttcataca
     4741 ctcacccctc tcatgcatct ctcgacaaag tataacgcca accacgagag ggtgcatctc
     4801 tcaggatcga tagtcttcaa aaccccggga aaggcagcaa atctcccgac tttcgcggct
     4861 ttttccagtt cctctttgag tgcgggcgct atgtaataga aggcacctga gatctttccc
     4921 ccgaatacta cgaggtcggg atgagagagc cctaagagga gcagaggatg ggtctgcaaa
     4981 ggtgactatc ccgcgtgatt gtgagagcga tctaaaccac gacttatgtc ttgaatcata
     5041 attttcggaa gacacgcggc ttttttacag atagatgcta cgatctcata gtaagagagg
     5101 cctttagagg gaacgaagaa tgtcgtgatt atcgactaag aagactgctc ttttcgccaa
     5161 agaaatcgcg gaagaaacgg gttgcctctc cattggcgag aggataagat acactttaaa
     5221 accttacagt ttcattcgag ttatcggaac ggagggatcg aagtgacttc aagagagagg
     5281 ataatcgaag cgctgaacca cagagaagcc gaccgggttc ccgtggatct tgggggtatg
     5341 agatcgaccg gcatacacgc aatggcttac cgaaggctca aagagtacat gggacttccc
     5401 gataggccgg taaagatctt cgacgtcttc cagcagctgg cgctcgtcga ggaagaggtg
     5461 agacggcggg tccattcgga cgtcgtcgag ctcaagaggc tcgacggcgg gttcaataca
     5521 agaatagata aatggaaaga gtacgatatg ttcgaagatg gcggaaggta tcttttaccc
     5581 gacggcttcg attccgtaac gatgcccgac ggttccaggg cgatcgtcag agagggcagg
     5641 atcgtggcca tcatgccgaa ggggggattc tatttcgatg gaaagcactt cccgctggcc
     5701 gatacgcatg atagaaaagc gatcgacgaa gccgtccgcc ccggtatttc cgacggagag
     5761 atcgcttttt taaaggacca gcttagagaa ctgaagagtt ccaccgactg cgccgtgatg
     5821 ggaactttcg gcgggaactt tctagaggcc ggccacagct actttggtta tcaggagttc
     5881 atggagagaa tgataacgga ccggccactg gtggagtact ttctcgatag acttctcgag
     5941 aagtacacga tcgaactcga gagatatctc ttcgaagtcg gcggagatat cgaccttata
     6001 cagatcggcg acgactacgg gacgcaggaa aacactcaga tctcgccccg gatattcaga
     6061 tcgctcttca agccacgcct taaggccctc tgtgatttca tccattcgaa gagacccggt
     6121 cttttcatct ttctccattc atgcggatca gtctatccct tcataggaga tttcatagag
     6181 gccggcgttc aggttctcaa ccccgtccag accaacgcga agaacatgga ccccgccatt
     6241 ctaaagcgag agttcggtgg tgagatcgtt ctctggggag gtggttgcga cacgcagcat
     6301 ctgttacttt tcgggactct ggaagagatc gaagaggacg tcaggaggcg catagatata
     6361 ctcgcacctg gaggggggtt cgtcttcgcc tcgatccaca atatacagat ggagatacct
     6421 ccggaaaaga ttctgcggct cttcgacacg gcctatgaat acggaagagg tgcgtacagt
     6481 gaccggaaaa gatagaaccg tactcgttat ctcacacaca ctgggacaga gagcgatgaa
     6541 aggagaggat acccgtccag tataccctga ggctaatgga gcccggcaga accgaaaaag
     6601 attgtaacgc tagaactggg gaccagcgat agactgacac tctccggttg gcaggagtaa
     6661 aggcccagac gtgttatcct ttgaataatt tctcaaggag gtgtctcgat gggtagaaca
     6721 cgttcaatca ctctagcgat cctttttctt ctgattgtct ccgcggctat ggccctgatt
     6781 gagctgaagg atttctctct ggcgagcata gatatgaaag agttccaggc agttcaaccg
     6841 atgtacacga tatcggccct gtccaggttc aacggcagat caggatcttc cgcttacgtc
     6901 tcggtcgaag gcgccatata cgatttctcc cagctgagac gctggatcgg gggcaatcac
     6961 atgtctcaac actcggcagg tacggagctg acgtacgagt tgaaaagtct ttctccccac
     7021 agagagggga agatcgcagg catagagcca gtgggagttc tggtggtcac tcttgaagag
     7081 cttaaaaact tcgatggaaa gagcggtaaa aaaggttacg ttgcggcctg gggaaaggtg
     7141 tacgatgtct cccgtctggg aaggtggcga aacggttcac accagggagg tatgcacttg
     7201 gccggagagg aattgacttc cgagctcaca aaggactcgc cccacggagt tcgcaagatc
     7261 gaaggagtcg atgccgtcgc cattctgggt gtgacaatcg acgagttgaa aacgatgaac
     7321 ggtaatggtg gaaagaaggc ctatgtggcc gtagagggta aggtttacga cgttagcgga
     7381 ctcgcctcat ggaagggtgg aagccatcag agcggacttc acctggccgg taatgagctc
     7441 accgtagaaa tactgcgaga ctcgccccac ggagcgacaa gtctcgacaa ggcttacctg
     7501 ataggaatac tggtcttcac gccggagcag ctgaagaggt acgacggtgt cgtggagagc
     7561 cgtaagttcg tttcgtacga aggtgtggtt tacgatgtgg ccggcatcga ctggacagcc
     7621 ggttacggaa tagagctcgg agcttcgctg ggaagcgaac tctcgaagct agaagatgcc
     7681 catataatag gatatgtggt gcgaaactga aagatccccg tttgaaatgc aagagaaaag
     7741 gctagagcga tgtcgtgaga ctgccttccg atccaatggt atgatggtat gaaagatgcg
     7801 ccggacgagc tccccataag tcttgtcgtg ggcgcttcat gggaacaata tccagaatat
     7861 cacgaaggac gggggtagtg ttgaatgttt caaagactca tagagggcag gcttctgata
     7921 gttatcagag ggctggatac gggaagatca atcgatctat gcgggctcct ttacgattcg
     7981 ggagttttct tcgccgaggt cagcttttcc gaccggtggg ccgacgaatc actcaaggaa
     8041 ctcaaaaaac acttcggcga caagctgctt ctgggcgcgg ggacgatctt caacgcagac
     8101 gattattcga gggcattgcg tgccggcgcc gattacatac tcagtcccgg tttcagccac
     8161 gaggtcgctg cgctttcgct gaaagatggc attccttata taccgggagt ttacacctcc
     8221 actgagatcc agatggcttt gaacatgggc tttacaattc ttaaactttt cccgggagag
     8281 cttgaagggc tccgtctttt gaaagcctac aggggaccgt tcccacaggc cagattcatg
     8341 ccttttggag gcgtcacacc cgagaacgcg gcggccttta tcgaagaggg tgcagccgct
     8401 ctcggtgtgg ggagttacat agccagcccg gagctcctcc aagagagaaa atacggcgag
     8461 atttcggaga gaatctccag aatgaagatt gcagcgggga taatcgatga ctgatctggc
     8521 gatagatgtc ggtggaacca agacgctggt ggtggtcttc gaagacgacc aggtgaagcc
     8581 gttgtatgtg gagagatttc caacggactc caccgccggg ccagagaatt tcatagccag
     8641 actggcgaag gtctgctcga aatacgtcga tcctccggat gtggtgcact ggggactttg
     8701 cacagccggg gcagtagatg tttcgaccgg taggttgata tggtctccca acctcggctg
     8761 gaaggatgta aatcttcacg aaaggctcag tcagcttttc ggggatcggg gcatcgtgga
     8821 aaacgattgt aacgctgccg cctacggaga gtggtcgacc cggccgggta tagattctct
     8881 cgtttacgta accgtcagca cgggcatcgg tatgggaata gtatcgtcgg gtcatataat
     8941 gcgcggctct cacggttttg caggagagat aggccacact acggtgcttc ctgacgggcc
     9001 ggaatgtgcc tgcggccgca ggggctgtct tcaggctgtt gccggaggaa ggggcctctc
     9061 catacaggta gagagattcc tcggcaagcg cctcgcgacg gaggagatag tcgaccttgc
     9121 cagagaaaac aaaccggagt acgtcgagat cgttgtcggg ggagccgcta ttctggggcg
     9181 ttttctagcc aacatgatcg atacgctcga cccggccgtg ctggtaatgg gcggcagcct
     9241 gggaaagaat ccccattacc gcaggctggt tgtggatttt tttacgaagt tctattacag
     9301 acttccagat aaaacagtcg gaatcgaacc gccctcagcg gagcccgatc cctgtataat
     9361 cggagtgttg cagttatcca gatcgatggg ggtgaggaag agtggataaa gcgatcaaat
     9421 attcaattca cgagggagat ttttccaggg gtatggaggg cttctcttct aaagcggtga
     9481 agatcgtcgg gcccttttcg ggtcgcccgg aggcacaccg cgaccagcac gacctttatg
     9541 tcgttatctc tggaaaggcc cgcgtcaaga caggaaaact taccggggat atccaggaga
     9601 tagccgacgg cgagtaccgc agcgacgaga tgctcgccag cgaggaattc acgctcgagg
     9661 agggcgaatc gctgctgatt cccgcttcgg tcgcccacac ggttctcgtg gattctggca
     9721 cttatgtcca gtgggtcttt aaaatcgatg cgtgaggcgc cgctctggaa ctagatcctt
     9781 cttacttcgt actcctggag gctgtgagtt tctccgccga tcttcaacct tatactgtgc
     9841 gggtaagggt tcaagataac cctgtaagac ttcatactca acagcctggc tgaaccttcg
     9901 gcttttcttt caatcgaagc ctcgaggaag agttttttta tcaattcgac gtccgggata
     9961 gtactcacgt agcagcagtt ccccttcacc gttacggcag gtttccccct gaaaggtccg
    10021 tctatgaacc tggctactgg ttggcagtca tcgagcgttt cgatctcctc ggccacgaag
    10081 ccacaatcac agacactctt gccggacgag actttgagcg ggccttcgaa tcctccgaaa
    10141 tcgacaacgc gaattcctgt cagaagctga aaatcgtcgg ccttctgcat ccggatgttg
    10201 ttgtttttat ccttctgggc cgtcatggcg gtgatgaata tcttcccttc gaagttcaac
    10261 agctccctga cgaagtcctc ctcgatcttg aaggccgaag ggacggcgac gaaggagtaa
    10321 ccctgcggcg actcgcctgg aaagagaaaa tcgatgttgt agccgaagct cctgaaggct
    10381 ttgtaaattg gcagtatcga gtcgaacagc aacctgaatt tgccgtttag gttgtcggtc
    10441 tcacctatcc agaaattctc gtagtctata tagatcgcgg cctctttttc cgggtcctca
    10501 atcttccagt ttcgcaactc ctcgatagtt tccgcgaaga ctctggccct ctccgtcatc
    10561 gttccgtcgt aattcaaaag accgctgtga aactgttcgg ccccgaacgg gagctgtctg
    10621 tacctgaaaa caagagaacc gaaggctccg tgtgcgacgg actgcttgat ccagaaggcc
    10681 agctgtccgg aagggtaggt gttgtttaca ggacgccagt tgacccttcc cggttgttgt
    10741 tccatcacca gaaagggagc cttcttcaga gatctcatca gatcgtggtt aagggcctgc
    10801 agatcgggat cgtactcccc ggccacgtag ttgtcccagg atacgaaatc gatccttttg
    10861 gcgagttttc tataatcgat atcggtgaaa ttgaccatga gattgtgggt cagcggggct
    10921 ttactgttct tcttgattat ctctttatga atgtcatgat attcgattac cgaatcggaa
    10981 gagaagcgtc tataatctaa taccatagag gggttgtatg atacaggacc gggtttggga
    11041 gggtttatct cttcccagtc gctacagatg ttgctccaga aaaccattcc ccacgcctgg
    11101 ttgagattgt ccagagttcc gtacttctct ctcaaccact ctctgaaagc tcctgcacag
    11161 ttgttgcagt aacatagcgc cgaaccatga cagccgtatt cgttatcggc ctgccaggcg
    11221 attattctgg ggtctttccc gtagtgatct acgtatatgc ctactatcct ctccgtctct
    11281 tcacggtaac gcggagagtt gaagcagtac tggcgacggc cagcggtgcc gggctttctt
    11341 ccatcaccgg ctaccctcag tacttcgggg tatttcttga gaagccacga aggcggagct
    11401 gccgtcggag tgcccattat gatccttata ccctcggaat acagcgcctc gatcgcttcg
    11461 tccagtagag tgaaatcgac ctcacctggc cgtttctcca ccacgcccca ggagaactcg
    11521 cctattctca cgacgttgat accgaggtat gccattactc ttgcgtcttc tttccatctt
    11581 tcccgggacc agtgttccgg gtaatacgcc gctccaaacc agttcaaact tcaacactcc
    11641 cttgaaaacc atccaggctt aaggtacgtt tgttgttatt atacaaaaaa ccggactata
    11701 ctttcgttga ttcatcagtg aatcgatgga aagagtcaca acgctggtcg cttttagcct
    11761 gtatggttgc ccgatttaga tcactgccct ttttctcccg ggatatccat ggcaaagagc
    11821 gctgcgccgt aggcgcccat tatatccggt acttcgggga cggtaatttt tctcttcaac
    11881 actctttcca tggagcgcac aactcctgcg tttctcgcca ctccaccggt gaagaccagc
    11941 ggttccggag agccgaggcg cacgtacatc gcggccactc ttttcacaat cgcgttgaat
    12001 aaaccggccg atatgtcctc aatcttctct cctctaccta tgagagacac aacttcggat
    12061 tctgcgaaaa cggtgcagac tgaactgatc gaaagctctc tggaggaacg gagtgcaagt
    12121 gaggccatct ctccagtatc cagttcgaga accttcgcca tgacctcgag aaagcggccc
    12181 gttccggcgg cacatctgtc gttcatggag aaatcctcca cgttcccttc tgcggaaagc
    12241 tttatcacct tgctgtcctg tcccccaatg tcaacgattc caatggccga cgggaagagc
    12301 ctcgcaacgg ctctggcgtg gcaggagatc tctgttaccg tttcatcggc gaagtcgatc
    12361 aattccctgc cgtaaccggt cgcgactatt cgtgctatct cttctttaga tccgccggac
    12421 tgcgagagca gctcttcata aagacgcctg gccgaagcca gagcgttaat tcccgtcctg
    12481 atggtcttcc agcgtagtat ctttcccgtc gaatcagtca atactccgtt ggtggttgtg
    12541 gaaccggaat ctatacctaa aaacaccgat ctttcacctc tttcaatttc tgatcgtcgg
    12601 gccaagttct tctccagcaa ggctcccact cttgtggcta tctgtccgtg ggagttgagg
    12661 gggtaatcga tctcggccct gtatatcttc gaattggagg tgttgcgcgt gaaatcgtag
    12721 aagtcgcaaa acttcggtat ggcgtacagc agcccgtcaa gtccctcgca ctcgtccttc
    12781 gattcttccg aaaatctccc gcacggcgcc cgccagccaa tcaacctctg cgccacatag
    12841 aacgccggat cttctccgac acgagttctg gatgcgaagg gccgggcacc caggcaggtg
    12901 tcgttacggg ttataacccc gcccgcactc tcgattatag cgtctatctc actcatgtcc
    12961 acgattccac ctgccagacc aaaatccttt cctgccgttt tgtttttgac cctcgagccg
    13021 gtcgggtcgt gcttcagacc gttcagagca gaaagataga gttccgttag gtcgcggcct
    13081 acgagcgttg actccagcga tcgaacgtgg tcgatcaggc cgttgaagcg ctccactcca
    13141 gccgatagat tcacgtcgat ccctttttcc tcgagaaact cgatcaggcg aaggatttcc
    13201 gaggccatga attctaccgc ccgttcatct ctcttccatg ggagacgcat catgaatact
    13261 cctgccctgg agctttcgct cagaaggtcg ccgaccctgc gcatcgcatc gcacgaatcg
    13321 gccaccagga gaagatcgcc cttatcaagc tccataccgg ctccgtaaac aattttggca
    13381 tagccgcata gattgcagcc cagctcgtgg caggacgacg aagccgcgct caacaacctc
    13441 tcgaagtctt taccggtacc cataaggatc tccagcggga tatagctgca cgtgtatagt
    13501 atcatctctt gccctccaga atctcgagaa aggcctgagc acgcgttctc aactgcccgg
    13561 ccccttctga ggtgtgatcg acacagtcgc ctgtcagctc caggaaggga atccctttag
    13621 attcgaacat cttcttgaga taactcacat ttcccgccga ctgcctgcat ccccagtggg
    13681 tgaagtgaac gatcccgtcg gctcggaaat cgtcggccag aaaggcgcat tgtttccacc
    13741 tgtcttcgac ccctccgcac aggggattga agacgagctt ctcggccaga ctctccagcg
    13801 gtctggaggg ttccaccgga tagagccagt cccacatcag ttcgttgccg accaccgtat
    13861 gcttcgattg cggcgaaaag atcttgaaca actcgttgtc gtagtagggc gggatgtgta
    13921 gccagagaat cttcttctgc tcagacctgt ccgtgatcag attccttccg aactcctcgg
    13981 atgccctctt tagcctagcg tcgccggcga gcgtgtgcat cacataaagc gagttcatgt
    14041 actcgtacag ctcctgcgga agatgagagc ttttcatctc ttttcgaacc cgcgtaagat
    14101 tttccaccgt ttgcctttcg atggcgagcg tatcactcag gaggttcatt ggataaggct
    14161 ttccagtaat ctcctctatg agccctatcg cctccttgag ctgtgaatgc acgtacccaa
    14221 cggagctctt catgcggccc cggggaacgt cgatcagtat gaagggcacc cggtattccc
    14281 gggagatcct cttgaaggta ggcaggttcc cgtcgcagaa gatcgtggag gtcatcacga
    14341 atctaggagc cggaaagagc ttcatgatgg aagccccgag ggaagccctg tgaaaagtgc
    14401 agagcgagtc tggaataccc gattcggcgg ccgaagatat cgcaaaacct tcgagatgca
    14461 tcgaggcaag catgcccgat actccctcgg ccgataccgg attgataccc agaggcagaa
    14521 gaagttccga aggggcgagc aggttgaccc aggcggtttc ccttccggtc agggagtaat
    14581 aaacctcttc gactgccaga ctgttgacga tccttagaaa ccgtgccttg cgcgagggta
    14641 agtagcgacg tctgaagttc tcgaaagcga ttcctagatt gagccacttt ctgaatttca
    14701 tttcatcgtt tatgtatcgt ctcaagaacc ttccgaatcc aacactcttc ttcgacatct
    14761 gctcccctat ctcttatatc tactggcttt ggtcttagaa ttatactcca ggaggataaa
    14821 agtgttttta cgatcgcccg cagggaatac agtaccacgg tccgcgttct ttatacacaa
    14881 tttgtaaagt aaaaaattga cattttgtct ataaatgtgc tagtattttt atcatcgacc
    14941 ttgagataaa tacagcggat aactctgccg tttttgacgc ctatccggcg aggcggtgga
    15001 caagacaata gaggcaaatg gagggtgagg atcgtgaaaa aacgagtgag cgttgttctg
    15061 attgcactga tagctattcc ggctttttgc ttcgcggccg ttgccgcacc tcagatcttt
    15121 acgtttgcgg gccataacga cgtgatgaca ctggatgtca gccagatgaa cgatgagatg
    15181 tctgccctgg ttatgtacgc gctgaacgag gccctgataa gaaatagcta caacaatatc
    15241 attcccggcc ttgccgggtc tttcgatgta tccgaagatg gtacggtcta cactttccat
    15301 ctccgcgacg caaagtggtc cgacggagtg ccggtgacgg caaaggattt cgaatactcc
    15361 ttccttcgaa cgcttaaccc tgagaccgga tcgtcacagg tttctgaatt cgactcggtc
    15421 ttgaacgcca aggcatatta tagcggcgag ataaccgata cctctctggt cggcataaag
    15481 gccttcgatg acagaacact gattataact ctcgcctaca gcgatccttt ctttatttcc
    15541 gagatggccg aaggtatcaa cttctacccg ataagaaagg attatgtcga gaaatatgga
    15601 atgtcttacg cctcaagcgc ccagacattc ataggttgcg gtcccttcgt gctcaaggaa
    15661 tgggtgcagg gcgccagcat cactctcgaa aagaatcccc tttactggaa tgccggcagc
    15721 gtgaagctag agaaagtaat cgagctgata attcccgacg agaatacccg cgtgggtatg
    15781 tacgatctgg gggagatcga cgctatctac tcgatctcga aggtgcagac gataatgcac
    15841 cccgagtacg gaagcagaag cggcggtact cttcagtatc tggcattcaa ctgctccgac
    15901 ggacttgtga tgagcaacat gaatctgcgc
//
LOCUS       DAOOLY010000020        15818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3920_length_15818_cov_5.027660, whole genome shotgun sequence.
ACCESSION   DAOOLY010000020 DAOOLY010000000
VERSION     DAOOLY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 15818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15818
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_15818_cov_5.027660"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLD15_02005"
     CDS             <1..207
                     /locus_tag="PLD15_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62701.1"
                     /translation="IKNGDLNDIKKLINDYFLGKDIPVWIGMPSYHGDFPKLVLPVGQ
                     WVSVDMEKGIIEVLNSPADMVEKK"
     gene            complement(251..1210)
                     /locus_tag="PLD15_02010"
     CDS             complement(251..1210)
                     /locus_tag="PLD15_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB62702.1"
                     /translation="MSFFELGSAKKAEIEDRYDIVIIGGGPGGITASIYAVQAGLKPL
                     IIEKAIDGGQMNNTQAVENWTGFTSVGGMELSEKMAEHARAFDVPFFYGEVVDVELEG
                     PEKAVILDNGRRVASRVIIVATGSNPRSLGVEGEEAFKGRGISYCATCDGHFFTDKHI
                     AVIGGGNSALDESIYLSKMIRKITIVQNLPKLTADKLLQDKLNATGKVEYIFSSVVES
                     IEGDTQVRAIKIRNQETGETTKLPIEGMFVFIGLLPNSGFLKGKLPLNRFGYIQTDGN
                     METEVKGVYAIGDVREKEVRQIVTAAADGAIAVYHAARTYFDG"
     gene            complement(1223..1870)
                     /locus_tag="PLD15_02015"
     CDS             complement(1223..1870)
                     /locus_tag="PLD15_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB62703.1"
                     /translation="MEKKLLPDNIIKQVKEVFAALEGPVKALFFRSEKKCDYCNTAEQ
                     LLNELSGINDKLAYEVFDVDSDMARKYEIELAPSIVLLTPDGDDKGVRFLGVPAGHEF
                     GTLLQDIVTFSRGAKPELSKESLEKIEAIDTPVEIKVFVTTTCPYCPKAVLTAHHIAM
                     ANKNIKAYMIEANEFQEMSMKYGVSSVPQIVINDRVTFVGAYPEGQYVEQVLKAL"
     gene            complement(1931..2482)
                     /locus_tag="PLD15_02020"
     CDS             complement(1931..2482)
                     /locus_tag="PLD15_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62704.1"
                     /translation="MLIQETIELLIILLLISALATVFAVATVFAFYLRVRRPPALLMF
                     MNSAASTTVTLLGSLVLTDNRKLFLTFLFFVAYFFIYNSLGRHITSYDDTFRILFLSM
                     GYTREEFFMNYLLVQGKWKLIESNISFASSSTFIIIFSGELARFFPDQWVGVTLVFAV
                     STFVLSVSRWIGEDFRNKLSKEE"
     gene            complement(2488..3420)
                     /locus_tag="PLD15_02025"
     CDS             complement(2488..3420)
                     /locus_tag="PLD15_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPB62705.1"
                     /translation="MIIVWLVLAAVIFIIAASGIKIIRPFEKGLVERLGKYRREAQPG
                     LQFIIPFIERMIKIDLRETVIDVPPQEVITKDNVVVTVDAIIYYQITDAFRVVYNVAS
                     FEIAAIKLAQTNLRNVIGEMELDQTLTSRERINVTLREVLDEATDKWGVKVTRVEIKK
                     IDPPQDIMDAMSKQMKAERTKRAVILEAEGYKQSEITKAEGDKMSAILQAEGQSESIK
                     RVAEANKFKLIAEAEGQADATISVFKAIHEGDPTKDVIAIRYLDALKQIADGKANKVF
                     LPYESSAMLSSIGSMAEIFREKSTSQERESTGEK"
     gene            complement(3441..3872)
                     /locus_tag="PLD15_02030"
     CDS             complement(3441..3872)
                     /locus_tag="PLD15_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPB62706.1"
                     /translation="MGYVGWMIFGLVLLVVEILTPTFFFLWFSIGAFLAGIVAMFTTN
                     VGWQIIVFAVSSALLVLLTRPIAKKLSKGDSPKKMYIDGLVGSTGRVVVEINPSMDRG
                     LVRIEGEDWRASSTDGGTIPVDTPVRVVRLEGTTIFVERKT"
     gene            complement(3908..4759)
                     /locus_tag="PLD15_02035"
     CDS             complement(3908..4759)
                     /locus_tag="PLD15_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB62707.1"
                     /translation="MEFQIFSKALYSTWILYRPERILFDSGEGISTILGNSVYAIKDV
                     FMTHGHVDHIAGLWGLINTRNTAMGDRNKPLRINYPAGNRAIEAFLDFIVGMNPRLRY
                     DMILNPLDDGDEVFLRTAGSFKRSVRPFRVKHTAGEISCGYHIYEERRRLKEELSGLT
                     SNEIASIVREKGRDAITDTYTQKIVTISGDTFALPPETISNSETLLHECTFLQEKDRR
                     NQNHSSVEEVIDSVKHSEGIKRLILYHISGRYTSKMKKWHSFVQKELEETGVEVYVVH
                     PEHVFTL"
     gene            complement(4761..5216)
                     /gene="rplI"
                     /locus_tag="PLD15_02040"
     CDS             complement(4761..5216)
                     /gene="rplI"
                     /locus_tag="PLD15_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPB62708.1"
                     /translation="MKVILLKDVQNVGKTGEVKTVSDGYGRNFLIPRGLAVEANQSEL
                     AKLNSEKKKKEEKEDRIKKASEELLSTLQKHHFIIKVKSGASGKLFGAVTSADIASHI
                     KNVLGLEIDRRHIDLAENIKQTGEYKVDIKLPGSVRGQIAVRVESLEEN"
     gene            complement(5265..5474)
                     /gene="rpsR"
                     /locus_tag="PLD15_02045"
     CDS             complement(5265..5474)
                     /gene="rpsR"
                     /locus_tag="PLD15_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPB62709.1"
                     /translation="MRSKKKCRLCGSKTTYIDYKNLSLLREYVTEKGKIIPKRITGNC
                     AKHQRMVKEAIQRARFMALLPYTKE"
     gene            complement(5514..5969)
                     /gene="ssb"
                     /locus_tag="PLD15_02050"
     CDS             complement(5514..5969)
                     /gene="ssb"
                     /locus_tag="PLD15_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPB62710.1"
                     /translation="MSISYNHVVLIGRLTRDPEIKFAASGTQIATFSLAVDRNIPSSG
                     NDNTDFIRIVAFGKTAEFVGNYLTKGRLILVEGSLRINKWKTQEGESRSNAEVVASNV
                     RFMETKAQTVSTGGFEKAPQEDSVVGSTGNDDITFFGSESEDTGDDIPF"
     gene            complement(5971..6423)
                     /gene="rpsF"
                     /locus_tag="PLD15_02055"
     CDS             complement(5971..6423)
                     /gene="rpsF"
                     /locus_tag="PLD15_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB62711.1"
                     /translation="MIRIYETMFIVSPRLDEESRNSMAEKVRDYIVERVSGTIEKFDR
                     WGIRKLAYRVTKGFSEGDYTVIQFKADSESVDILEKFYGITPEVFRWQTFRREDLEKA
                     EKKALLKKGTSEEELPEGQTEVKAVESAVVEVSEALVEKTAETVATEE"
     gene            6655..7128
                     /locus_tag="PLD15_02060"
     CDS             6655..7128
                     /locus_tag="PLD15_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPB62712.1"
                     /translation="MRTVIIFASKSGTAEKCSRMLSEKLPGEVKVIDIGKNPNPGFSD
                     CDNVIIGASVRAGQIQKPIKEFVGKNLNSLKSKNVGVFLCMGGDRPQFEEYLKQNFPG
                     EFLDSCKAKGFFGGEFNMERLGFFSRTIIKALSKGKPTPKLFEENIQSFADSFKS"
     gene            7138..7737
                     /locus_tag="PLD15_02065"
     CDS             7138..7737
                     /locus_tag="PLD15_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB62713.1"
                     /translation="MRGSEIRRERNREYFIKAARQVIEERGIKALNIRRVSELAGYSP
                     ASVYNYFGSMDGLIGAVKDSYAVDAMEYISARLTDSMTPLERLETSYRYFAEYFLDRP
                     EIFKLIFMSGFGKGTDREAAPNFTEMAHQRMDLFHSVMKDHNVGEATLARVENILSSM
                     LFGNLYFLSFGLTDIDRDTLLENVSGNVSFVMDILGGSE"
     gene            7734..8147
                     /locus_tag="PLD15_02070"
     CDS             7734..8147
                     /locus_tag="PLD15_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB62714.1"
                     /translation="MSKRKSLIRVLVVIAIIAAAAIIVFVTVVIPKLEEGLKVLDTIE
                     FGSLNLASVNDGSYEGSYSAGIVKATVRVKVENHAITDIDILKHDNGKGKAAETITAA
                     VIKSQSVQIDVITGATYSSKVILKAIENAVTSSRQ"
     gene            complement(8360..9097)
                     /locus_tag="PLD15_02075"
     CDS             complement(8360..9097)
                     /locus_tag="PLD15_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62715.1"
                     /translation="MKMSSFAIRNAKELIRDRLNLAFGIGFPVIVLLMLTLIQSNVPV
                     ELFSIKNLTPGIAVFGLSFISLFSGMIIAKDRTSSFMLRLFTSPMKARDYILGYTLPL
                     IPMAALQISVCFIVAVLLGLEISVNMLLALIVLMPAALMYIAIGLLCGSIFTDKQVGG
                     VCGALLTNGSAWLSGAWFDVDLVGGAFKAIANALPFLHAVNAGRYALNGEYGRIMPEL
                     AWVIGYAVALTALAIIAFTKKMNSDNP"
     gene            complement(9084..9824)
                     /locus_tag="PLD15_02080"
     CDS             complement(9084..9824)
                     /locus_tag="PLD15_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB62716.1"
                     /translation="MTAIETKNLSKTYKATKAVEDLNLTIEKGELFALLGVNGAGKTT
                     TIKMLSCLIRPSSGDATLMGNSVVTSPLKVKEIINVSPQETAVAPNLSVRENLELMCG
                     IYGYDSKTAREKTGKTIKDFSLSEIEKSRARILSGGWQRRLSIAMAIITEPEILFLDE
                     PTLGLDVLARRELWRIIEKMKEKITIVLTTHYLEEAEALADRIAIMSRGKLVAVGTAE
                     ELKTLANAGNFEDAFVALCGGETTYENE"
     gene            complement(9821..10123)
                     /locus_tag="PLD15_02085"
     CDS             complement(9821..10123)
                     /locus_tag="PLD15_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPB62717.1"
                     /translation="MKAKSENNGKYMGSVKVGQKGQIVIPKEVREMFEISPGDTLVLL
                     ADSEKGIAIERYTVFARIADWILSGRAKEVYPDNSEEDSIKFAEGIKSIEDEGDEK"
     gene            10316..11605
                     /locus_tag="PLD15_02090"
     CDS             10316..11605
                     /locus_tag="PLD15_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB62718.1"
                     /translation="MKGREGVEMQQDRFYRLSGNPKNIHILRKIVDAAREDERIVSVV
                     SYGSYTRDDFDRFSDIEFYFFIRDDSAPGFDDELWLREVAPVEFYFTNSHGVKTAVFT
                     KFFVRGEFHFHPESEIAILDGWKGVALPGEVERTVLVDKSGAFRKKIEEFCQTRPVLD
                     KKASFKQNFLELASGIIMEWNVLSRKDLFRASTLHSMNLHYLARLFSLVHGKTDHLYS
                     PRLLERFMSEGEYRSFSECFAGLDFDSLRYALEKMALLSAELSRKNGDTDTVKARRAI
                     LERVVERSYRVEGVITDGEKVLFIKHSGNDGRPDEWLLPGGGKVAGESDEMAVKREVL
                     EETCLAIEPAGMIAEVELPDDGLYYSAKMFHFIYDGSEPSPGSEPEDVNGNYYTISEV
                     KWIDLSDLSSIADEYRTFPHMSERLEAIRDHLLKMNH"
     assembly_gap    11733..11832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11833..12635
                     /locus_tag="PLD15_02095"
     CDS             <11833..12635
                     /locus_tag="PLD15_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPB62719.1"
                     /translation="NNHRDKDFELFANGCFATDDSVMTLAVAKALMETEKNVDTSIND
                     YRLDGNYHRLLGELAVRYMREIGRKYPDCGYGGMFARWIFSDDPKPYRSFGNGAAMRI
                     GPAGFIARTETEAVKLSETITSVTHNHREGIKGAEATSVAVYLARRGFSKGEIRDRIS
                     KSYYSLDFTIDAIRPSYRFNEICQETVPQAIECFLESDSFEDAIRTAISLGGDSDTIA
                     AITGAIAQAHYGVPDYIGARALTYLDEELRAIYDEWEAFSQGWRPRIT"
     gene            complement(12697..15405)
                     /locus_tag="PLD15_02100"
     CDS             complement(12697..15405)
                     /locus_tag="PLD15_02100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB62720.1"
                     /translation="MNSRKDFSIKNAIRIVFILAMLLSIGGIGYLIFSGWLSSAEKTA
                     GSIVETIGEGIYNRVVSFMHEPDHINDANRKIIENGILDLYDEESRDKFFVGVLSSQQ
                     EEIYSFSYGTENGEYYGARRNEKGVIEIMRNDVSTGGNSWYYSVNEDLTAGERVVVAG
                     KFDPRTRAWYKAVQEAAGPAFSPIYKHFVMSDLTISAAWPIYDSDGNLRGVMGTHVLL
                     SSVGAFLRDTVNNYDGYAILFEKDTGALIANSTGVDNFSVLSDGTLKRNTISEMEDRD
                     IQQEYAQYATSGDSIFIYGSGREKLFVNVREIQMPGIDWILISAIPEGLYMSSVVESV
                     YLTALLVAFALVVSLIAYHIVIGRLIRPIDDLLDVSAALSSGDLSRRAEVVRNDEIGS
                     ISESLNRVADKMQFLINNLEESVEERTEQLYGANVALEENKNQLQLILDSTAEGIYGI
                     DMDGRCTFCNISSVRMLGYSSPAELLGKNMHLQIHHSYRDGRPFPIDECRIFMSIRQG
                     KGFEADDEVFWRADGTCFDVEYHSYPQIRNGEVVGGVITFMDITDRKKREAEIEYLSC
                     HDVLTGLHNRSCFENSRDKIDIPDNLPLSVVFADINGLKMTNDVFGHTAGDELIKKSA
                     EILRQSCRKGDVIARVGGDEFVILLPKTDMGDAEKILSQIKTGFSNARIEAIKCSVSL
                     GIDTKTNSYQSLEETMANAENMMYRDKTINRKSANRDIIDTIIETLHSKYPREKQHSL
                     AVSRLCGEFGIALNLSKPEINVLKRAGYLHDIGKIVLKESLLSKDHLKAFTEEEREKV
                     KQHSVVGYRILNLFDETLDLAEYAYSHHERWDGKGYPRGLEGEQIPLIARIISIAETY
                     DRVLNRGDGPFEERKRAALKAIKDSAGKQFDPELAGLFVQTMDKSA"
     gene            complement(15632..>15818)
                     /locus_tag="PLD15_02105"
     CDS             complement(15632..>15818)
                     /locus_tag="PLD15_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB62721.1"
                     /translation="GDIVSIAHKLGHLVVAEGVEHEKQREYLSEYGCDNIQGYLISRP
                     LDEEAAIEFLKTYNRQR"
BASE COUNT         3581 a         4156 c         3844 g         4137 t
ORIGIN      
        1 ataaagaacg gcgatctgaa cgatatcaaa aagctgataa acgattactt tctcggcaaa
       61 gacatccctg tctggatcgg tatgccgagc taccacggag attttccgaa gcttgtgctt
      121 ccggtgggcc agtgggtgag cgtcgatatg gagaagggga tcatcgaagt actcaattcc
      181 cccgccgaca tggtggaaaa gaaataagag cgcccggagg cgctcttatt actctataca
      241 ggtttttcga tcaaccgtcg aaataggtcc tggccgcgtg gtaaacggcg atcgccccgt
      301 cggcggcggc cgtgactatc tggcgcactt ccttttccct gacgtcgcct atagcgtaaa
      361 cgcccttcac ttcggtctcc atgttcccgt cggtctgtat gtagccgaac ctgttcagcg
      421 gcagtttccc cttgaggaag ccgctgttgg gcagaagtcc tatgaagacg aacataccct
      481 cgatcggcag ttttgtggtt tcgccggttt cctggttcct gatctttatg gcgcggacct
      541 gcgtgtcgcc ctcgatcgat tcgaccacgg agctgaaaat gtactcgacc tttccggtag
      601 cgttcaattt atcctggagg agtttgtcgg ccgtcagctt cggcaggttc tgaactatcg
      661 ttatcttcct gatcatcttg ctcaggtata tagactcatc cagcgcgctg ttgccaccgc
      721 cgatcacggc gatgtgtttg tcggtgaaga agtggccgtc gcaggtggcg cagtaactta
      781 tacccctacc cttgaaagcc tcttcacctt ccacacccag acttctaggg ttggaacccg
      841 tcgctacgat gatcactctg gaggcgaccc tcctgccgtt gtccaggatc acggcctttt
      901 ccggtccctc gagttctaca tccacgacct cgccgtagaa aaaaggcaca tcgaaggctc
      961 tcgcgtgttc ggccatcttt tcactaagtt ccatgccgcc gacgctggtg aaacccgtcc
     1021 agttctccac cgcctgggtg ttgttcatct ggcctccgtc gatcgccttt tcgattatca
     1081 gcggtttcag accggcctgc acggcgtaga tggaggctgt gataccacca ggtccgccac
     1141 cgattataac tatgtcgtat ctgtcttcaa tttccgcttt tttggcgctt cccagctcga
     1201 agaacgacat ctctttccct cctcagagcg ctttcaatac ctgttctaca tactgacctt
     1261 cggggtaggc tccgacgaag gtaactctgt cgttgataac tatctgcgga acggatgaaa
     1321 caccgtactt catggacatc tcctggaact cgttggcctc gatcatgtaa gccttgatgt
     1381 tcttgttggc catggcgatg tggtgggccg tcaatacggc ctttgggcag taagggcagg
     1441 tcgttgtaac gaagactttt atctccacgg gtgtgtctat cgcctctatc ttctcgagcg
     1501 attccttcga cagttcgggt ttcgcacccc tcgagaaggt cactatatcc tgcaggagcg
     1561 ttccaaattc gtggccggcc ggcacaccca ggaacctgac tcccttgtcg tcgccatcgg
     1621 gtgtgaggag cactatgctc ggcgccagct caatctcgta cttgcgggcc atatcggaat
     1681 ccacgtcgaa gacctcgtag gcgagtttgt cgtttatacc cgacagctca ttcaaaagct
     1741 gttcggccgt attgcagtaa tcacatttct tctcgctcct gaaaaaaagc gccttcaccg
     1801 gtccttctaa cgccgcgaat acctctttca cctgtttaat gatattgtct ggcagcagtt
     1861 tcttctccat ctctatccct cctaatatgg ttgtataaat accctatagg ggtataatac
     1921 cataagtata ttactcttcc tttgatagtt tgtttctgaa atcctccccg atccatctac
     1981 tcacactcaa aacgaaggtg gaaaccgcga agacgagtgt gacgccgacc cattgatcgg
     2041 gaaagaaccg ggccagttct ccggaaaaaa tgatgataaa tgtcgagctt gatgcgaagg
     2101 agatgttgct ctctatcagt ttccacttcc cctggacgag caggtaattc atgaaaaact
     2161 cctccctggt gtaccccatg gaaaggaaga gtatcctgaa agtatcgtcg taactggtta
     2221 tatgtctgcc gagagagttg tagatgaaga agtaagccac gaaaaagagg aaagtcagaa
     2281 ataatttcct gttgtcggtc aaaaccagcg agccgagcag cgtaacggtc gtggaagccg
     2341 ccgagttcat gaacatcagg agagcgggcg gtcttctgac acgcagatag aaggcaaaaa
     2401 ccgtggccac cgcaaatacg gtggccaggg ccgatattaa gagcagtatg atgagtagct
     2461 cgatggtttc ctgtatcagc aatcagatca cttctcgccg gtactctccc gttcttggga
     2521 ggtcgacttt tccctgaaga tctcggccat agaaccgata gagctcaaca tggcgctgct
     2581 ttcgtaaggc agaaagacct tgttggcctt tccgtcggcg atctgtttca gagcgtcgag
     2641 gtagcgtatc gcgatgacgt ccttcgtcgg gtcgccttca tggatcgcct tgaagacgct
     2701 gattgtggcg tcggcctgac cttcggcctc ggctatcaat ttgaatttgt tggcctcggc
     2761 caccctcttg atcgattcgc tctgaccttc ggcctggagt atcgccgaca tcttgtcgcc
     2821 ttcggccttg gttatctccg actgtttgta accctctgcc tctagaatta cggccctctt
     2881 cgttctttcg gccttcatct gcttggacat ggcgtccatg atgtcctgtg gcggatctat
     2941 cttttttatc tcgaccctcg tgaccttgac gccccactta tcggtggcct cgtcgaggac
     3001 ctctctcagg gttacgttta tcctttccct tgaggtgagc gtctggtcga gttccatttc
     3061 gccgataacg ttcctgaggt tagtctgcgc aagctttatc gcggcgatct cgaagctggc
     3121 tacgttgtaa actaccctga aagcatcggt gatctggtag taaattatcg catcgaccgt
     3181 cacgacgacg ttgtccttgg tgatgacttc ctgcggcggg acgtctatca cggtctcgcg
     3241 gagatcgatt tttatcattc tctcgatgaa gggaattatg aactgcaaac ccggctgggc
     3301 ttccctccgg tactttccaa gcctttctac cagacccttt tcgaagggtc ttattatctt
     3361 tatacccgat gccgcaatta tgaatataac tgccgcgagt acaagccata ctatgatcat
     3421 ctctcactcc tcccctactc ttatgttttt ctttccacga atatcgtggt tccttcgagc
     3481 ctcaccactc tcaccggtgt atcgacgggg atcgttccgc cgtcggtcga gctggccctc
     3541 cagtcttctc cctcgatcct caccaggcct ctgtccatag agggatttat ctcgaccacc
     3601 actctgccgg tagatcccac aaggccgtcg atgtacatct tttttgggga atcgccctta
     3661 gagagctttt tcgctatcgg tctggtgagc aacaccagaa gcgcgctgct tacggcgaag
     3721 actattatct gccagccgac gttggtggtg aacatcgcta cgatacctgc caggaaggct
     3781 ccaatggaga accacaggaa aaagaacgtc ggggtcagta tctccacaac cagtagtacc
     3841 aaaccaaata tcatccaacc tacgtaaccc atgctttacc tcctcccgga gtactgtgca
     3901 caggttttca caatgtgaaa acgtgttccg ggtgaactac gtacacttcc actcccgttt
     3961 cttcgagttc cttctggacg aaggaatgcc atttcttcat tttagaagta taccttcccg
     4021 atatatgata caatatcagt ctctttattc cctcactgtg tttcacacta tctatgacct
     4081 cttcgacgct tgaatggttc tggtttcgcc tgtctttttc ctgaagaaaa gtacattcgt
     4141 gtaaaagggt ctcggagttg cttattgtct ctggaggaag agcgaaagtg tcgccgctta
     4201 tcgtcactat cttctgggta taggtgtctg ttatggcatc cctgcccttc tcacgaacta
     4261 tcgaggctat ttcattcgac gtcagacccg agagctcttc cttcagcctg cgtctctctt
     4321 cgtaaatgtg gtagccgcaa ctgatctctc ctgccgtgtg cttaaccctg aagggtctta
     4381 ccgatctttt gaagctgccg gccgttctca gaaaaacctc gtcgccgtcg tccagcgggt
     4441 tgaggatcat gtcgtatctc aatctcgggt tcatgccgac tatgaaatcc agaaaggcct
     4501 ctatagccct gtttccggcc gggtagttta ttctgagggg tttgttcctg tcgcccatgg
     4561 ctgtgtttcg ggtgttgatg agtccccata gtcccgcgat gtgatcgaca tgtccatggg
     4621 tcatgaacac gtcttttatg gcgtagacgc tgtttccaag tatggtggag ataccctcgc
     4681 ccgaatcgaa gagaatcctt tccggtctgt agagaatcca tgtagagtac agcgctttcg
     4741 agaagatctg aaactccaat tcagttctcc tccaggctct ctacacgaac agctatctgc
     4801 cccctgacac ttccgggaag ctttatgtca actttgtact cgcccgtctg cttgatgttc
     4861 tcggccagat ctatgtgtcg tctgtcgatc tccagtccga gaacgttctt tatgtgggaa
     4921 gctatatcgg ctgaagtcac cgcgccgaaa agcttgccgc tggcgccgga tttcaccttt
     4981 attatgaagt ggtgtttctg gagagtgctg agcagttcct cgctcgcctt ctttattctg
     5041 tcttcctttt cttccttttt cttcttttcg gagttcaact tggccagctc gctctggttg
     5101 gcttcgaccg ccagacccct gggaatcagg aagtttctgc cataaccgtc ggaaacggtt
     5161 ttcacttcgc cggtttttcc aacgttctgg acgtccttta gcagtatgac cttcatagaa
     5221 accctccttt aaaaaagggg aggctccagg cctcccgttt tcgtttactc cttggtgtaa
     5281 ggaagcaaag ccatgaacct ggcacgttgt atggcttcct tgaccatcct ttgatgcttc
     5341 gcgcaatttc ctgtgatcct cttgggtatt atcttgccct tttcagtcac gtactcgcgc
     5401 agaagggata aattcttgta atctatgtag gtggttttcg agccgcagag tctgcatttc
     5461 ttcttgcttc tcattcttct cctgttgtct cttaccatgt cgttcctcct gtatcaaaac
     5521 gggatatcgt cgcccgtatc ttcagattcg ctgccaaaaa aagttatatc atcgtttccg
     5581 gtgcttccaa ccacgctgtc ctcctgcgga gccttctcaa atcctccggt cgaaacggtc
     5641 tgggccttcg tctccatgaa acgaacgttg gaggccacga cttcggcgtt cgaacgcgac
     5701 tcgccttcct gcgtcttcca cttgtttatt ctcaacgagc cttccacgag gatgagacgt
     5761 cccttggtca ggtagttgcc gacgaactcg gccgtcttgc cgaaggcgac gatccttatg
     5821 aaatcggtgt tgtcgttgcc ggaagagggt atatttctat ctactgcgag ggaaaaagtc
     5881 gctatctgag taccgctggc ggcaaacttg atctccgggt cccgggtgag cctgccgatc
     5941 agaacgacat gattgtagct gatagacatg ttattcctcc gttgccacgg tttcggcggt
     6001 cttttctact aacgcttcgg aaacttcaac caccgcggac tcaaccgctt tcacttcggt
     6061 ctgcccttcc ggaagctctt cttcggaggt tcccttcttg agaagtgcct tcttttcagc
     6121 cttctcgaga tcttctcttc tgaaagtctg ccacctgaag acctccgggg tgattccgta
     6181 gaacttctcg aggatatcta cgctctccga atcggccttg aactgaataa cggtgtaatc
     6241 tccctcggaa aagcctttgg ttaccctgta agccagtttc ctgatacccc atctgtcgaa
     6301 cttctcgata gtaccgctta cacgttcgac gatgtagtct ctcacctttt cggccatcga
     6361 gtttctgctt tcctcatcga gccgaggtga gacgatgaac attgtttcgt agatccttat
     6421 catttattgc ctcctccctt ggactgtggc cctgccttta aggcaaagcg ggaatttcaa
     6481 cgcattgaat tataacatac caccggaata ctgcaagaga agttttcttt tcgaaggaaa
     6541 ggctattgga atgaagattt ttcctttccg aaacacagcg gaggagagac ggaattaaac
     6601 ttgcgtcata aattgaacaa tgttcagtat ctttacaaca caggaggaac tatgatgcgc
     6661 acggtaataa tcttcgccag caaaagcgga actgcggaaa agtgttccag aatgctctcg
     6721 gaaaaactcc ccggagaggt gaaagtgatc gatatcggga aaaatcccaa tcccgggttt
     6781 tctgattgcg acaacgtcat aatcggagcc tctgtcaggg ccggccagat acagaagccg
     6841 atcaaagagt tcgtcgggaa aaacctcaac tctctcaaat cgaaaaacgt cggagtcttt
     6901 ctctgcatgg gcggcgaccg gccacagttc gaagagtacc tgaagcagaa cttcccgggc
     6961 gagtttctgg attcatgcaa ggcgaaaggc tttttcggcg gcgagttcaa catggaacgc
     7021 ctcggtttct tttccaggac aattatcaag gccctctcga aggggaagcc gaccccgaag
     7081 ctcttcgagg aaaacattca atccttcgcc gattccttca aaagctgagg tgatcgggtg
     7141 aggggaagtg aaatccgtcg cgaaagaaac agagagtatt ttataaaagc ggcgcgtcag
     7201 gtaattgaag aaagagggat caaggccctt aatatcagga gagtcagcga gctggcagga
     7261 tactctccgg cctcggttta caattacttc gggagcatgg acggtctgat aggtgcagta
     7321 aaggacagtt acgccgtgga cgctatggaa tacataagcg ctcgccttac agacagcatg
     7381 actccgctgg aaagactgga aaccagctac agatatttcg ccgaatactt tctcgacaga
     7441 ccggagatct tcaaactgat tttcatgagt ggattcggga aaggcaccga tcgggaggcc
     7501 gcgcccaatt tcaccgaaat ggcccatcag agaatggacc tctttcactc ggtgatgaaa
     7561 gaccacaacg tgggtgaagc gactctcgcc agagtcgaga atattctctc ttcgatgctg
     7621 ttcggcaacc tgtattttct aagcttcggc ctgacagaca ttgatcgcga tactctgctt
     7681 gaaaacgtat cgggaaacgt atctttcgtt atggacattc tgggaggaag tgaatgagca
     7741 aaagaaaatc gctaatcagg gtactggtag ttatcgcaat aattgcggca gctgcgatca
     7801 tcgtgttcgt gaccgtggtg attccgaaac tcgaggaagg cctcaaggtg ctggatacca
     7861 tcgagttcgg cagccttaat ctggcatctg tgaacgatgg aagttacgag ggcagctaca
     7921 gcgccggcat tgtaaaggca accgtgaggg tgaaagtcga aaaccacgca atcacggata
     7981 tagatatcct caagcacgac aacgggaagg gaaaggcggc cgaaacgata accgcggctg
     8041 ttataaagag ccagtcggtt cagatagatg ttataaccgg cgcgacttac agtagcaaag
     8101 ttatattgaa ggccatcgaa aacgccgtga cctcctcccg ccagtaaaga tccgggaggg
     8161 gtctattgaa gtaattctgt cttcttaatc tctgttttta ccaaaaacct ggacgcacag
     8221 cgtcggaacg caaagacgac agaggtaaga gggggagagg tcagaggtaa gaagatcaag
     8281 accttcacgc ttttcacacc tcctgcctct tacctctagc ctcaggttcc gctctacgaa
     8341 ggacggccct tcccgctctt cacgggttgt cgctgttcat ttttttcgtg aaagcgatga
     8401 ttgcaagtgc agtgagcgcc accgcataac cgattaccca ggccagttcc ggcattattc
     8461 tgccgtattc cccgttcaag gcatatcgac cggcatttac cgcgtgtaaa aaaggcaaag
     8521 cattcgctat cgccttgaaa gcaccgccga ccagatcgac atcgaaccat gcgcctgaaa
     8581 gccaggcaga gccattggta agcagcgcgc cacaaacccc gccgacctgc ttgtctgtaa
     8641 aaatgcttcc acaaagcaaa cctatcgcga tgtacattaa tgccgccggc atcaacacga
     8701 ttagtgccag gagcatattg acactgattt ccaatcccag cagcacggct acgatgaaac
     8761 aaacggatat ttgcagcgcc gccatgggaa tcaaaggcag agtgtatccc agtatgtaat
     8821 ctctggcctt catgggagat gtgaaaagtc tcaacataaa agagctcgtt cgatccttcg
     8881 cgatgatcat tcccgagaac aaagatatga acgaaaggcc aaagaccgcg attccgggag
     8941 tgagattctt gatggagaag agttccacag gcacgttgga ctgaatcaag gtaagcatca
     9001 gcagaacaat tacggggaaa cctatgccga aagccagatt gagtctgtct ctgatcagct
     9061 ccttggcgtt tctgattgcg aaactactca ttttcataag tcgtttctcc cccgcacagc
     9121 gcaacaaagg catcttcaaa gttaccggca tttgccagtg tcttcagctc ttcggccgtt
     9181 ccgacggcga ccagcttgcc gcgagacatg atcgcgattc tatcggccag cgcctccgct
     9241 tcttcaaggt aatgtgtagt gaggacaatc gtgattttct ctttcatctt ttctatgatg
     9301 cgccagagtt cacggcgtgc aagtacgtca agaccgagcg tgggctcatc tagaaagagg
     9361 atctcaggtt ctgtgatgat agccatggcg atggaaagcc tacgctgcca gcctccggat
     9421 agtatcctcg ctctgctctt ttcgatctcc gaaagcgaga agtctttgat tgtcttgccg
     9481 gtcttctccc ttgccgtctt cgaatcgtaa ccataaattc cgcacatgag ttcgagattc
     9541 tcgcgaacag acaggttggg agccaccgcc gtctcttgag gcgatacgtt tatgatttcc
     9601 ttcaccttga gcggcgaggt gacaacgctg tttcccatga gtgtggcatc accgctgctc
     9661 ggtcttataa ggcaggaaag cattttgata gttgtagtct tccccgcacc gttgaccccg
     9721 agtaaggcga acagctcacc tttttcaatc gtaaggttaa gatcttcaac cgccttcgtt
     9781 gctttgtatg tctttgacag gttctttgtc tcgatagctg tcatttctcg tcaccttcat
     9841 cttcgatcga ttttatgcct tcggcgaatt ttatcgagtc ctcttcgctg ttatctggat
     9901 agacttcctt tgccctgccc gatagtatcc agtccgcgat cctggcaaaa acggtatagc
     9961 gttctatggc tattcctttc tcagagtctg ccagaagcac cagagtgtct cccggggaga
    10021 tctcgaacat ctctctaacc tcttttggaa taacgatctg acccttttga ccgactttca
    10081 ccgagcccat atacttaccg ttgttttcgg attttgcctt catgattcat cagcccctct
    10141 taatttatca tcatcgtcgt catgtgcaac ttgtataact agtatgactt gtactactaa
    10201 taatacttgt tgtcttctga attgtcaata gagaccctat aattcagcgc ggacaaaggc
    10261 gtgatattat cttttttgta aaaggatgca atcgagacac tgattcgaac gtagtttgaa
    10321 gggaagggaa ggagtggaga tgcaacagga tagattctac aggctgtcgg gtaaccccaa
    10381 gaatattcat attttgcgca aaatcgtcga tgccgccagg gaagacgaga gaatcgtctc
    10441 ggtagtctct tacggttcat acacgagaga tgacttcgac aggttctccg atatagagtt
    10501 ttactttttc ataagagacg attcggcgcc cggtttcgac gacgagctgt ggctgaggga
    10561 ggtcgctccc gtggagttct attttaccaa ctcacatggc gtgaagactg cggtcttcac
    10621 caagttcttc gtcagagggg agttccactt ccacccggaa agcgagatcg ccatactcga
    10681 tggctggaag ggagttgcac ttcccggtga agtagagaga accgtgctcg tcgataaatc
    10741 cggggcattt agaaagaaga tcgaggagtt ttgccagacc agacctgtgc tggataaaaa
    10801 ggcctcgttc aagcagaatt ttcttgaact ggctagcgga atcatcatgg agtggaacgt
    10861 tctcagcaga aaggatcttt tcagggcgag tacgctacac agcatgaatc ttcactatct
    10921 cgccagactt ttctcgctgg tgcacgggaa aactgaccat ctctattcgc cgaggcttct
    10981 cgagagattc atgagcgagg gtgagtacag gagcttctcc gaatgcttcg cggggctgga
    11041 tttcgactca ctgagatacg ctctggaaaa gatggcactg ctctcggcag aactctcccg
    11101 aaagaatgga gacaccgaca cagtcaaagc caggagagcg atccttgaaa gagtcgtgga
    11161 gagatcttac agggtggaag gtgtaatcac ggacggagag aaggtgcttt ttataaagca
    11221 cagcgggaac gacggacgac ccgacgagtg gctgctcccc ggcggtggaa aggttgccgg
    11281 ggaatccgac gagatggcgg tgaagagaga ggttctcgaa gagacctgcc tggctatcga
    11341 gcctgcgggc atgattgccg aagtcgagct gccggatgat ggcttgtact actcggcgaa
    11401 gatgttccac ttcatatatg acggttcgga accatctccc ggctcggaac cggaagacgt
    11461 gaatggaaac tactacacca tctccgaggt taagtggatc gacctcagcg atttatcttc
    11521 gattgctgac gaatacagaa ccttccccca catgtccgaa aggctcgagg cgataagaga
    11581 tcatctgcta aagatgaatc actgatacag gaaaggggtg gtgtgaaaac tatgctggga
    11641 gcgatcgttg gcgatattgt gggatctaga ttcgagttca acaaccaccg cgacaaagat
    11701 ttcgaactgt ttgcaaatgg ctgctttgcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nntcaacaac caccgcgaca aagatttcga actgtttgca aatggctgct
    11881 ttgccacaga cgacagcgtg atgactctag cggtcgcaaa ggcgttaatg gaaacggaaa
    11941 agaacgtcga tacatcgata aacgactaca ggctggacgg caattaccac cggctgctgg
    12001 gcgaattggc ggtcagatac atgagggaaa tcgggcgtaa ataccccgat tgcggttacg
    12061 gcgggatgtt cgcccgctgg attttcagcg acgacccgaa gccgtacagg agttttggca
    12121 acggggcggc catgcgtatc ggcccggccg gatttatcgc tagaacggaa accgaagcgg
    12181 tgaagctttc cgaaacgata acctccgtca cgcacaacca cagagaaggc atcaaagggg
    12241 cggaggcgac atcggtcgcc gtctatctgg cgcgtcgagg cttttcgaaa ggcgaaatcc
    12301 gcgacaggat ctcgaaaagc tactactccc tcgatttcac gatcgacgcg atccgaccgt
    12361 cgtacaggtt caacgagatc tgccaggaga ccgtgcctca ggccatcgaa tgttttcttg
    12421 aatctgattc gttcgaggat gcaatccgca cggcgatatc gctcggcggc gacagcgata
    12481 cgatcgccgc aataaccggc gcgatcgccc aggcgcacta cggcgtgccc gattatatag
    12541 gcgcgagagc gctgacttac ctggatgaag aacttagggc catatacgat gaatgggaag
    12601 ctttttccca gggatggcgg ccgcgaataa cgtgacggcc ttcctcgcta gaccgttctc
    12661 ttcgtccagt ccgttagcgg ctgccgaaca gcaggctcag gcactcttgt ccatcgtctg
    12721 aacaaacaga ccggcgagtt ccggatcgaa ttgctttccg gcgctatcct ttatggcctt
    12781 tagggccgct ctctttcttt cttcgaaggg gccgtctcct ctgttgagta cgcgatcgta
    12841 ggtctccgct atcgatataa ttctcgctat cagcggaatc tgttcgcctt cgagccctct
    12901 tggatagcct ttgccgtccc acctttcgtg gtggctgtag gcatattcgg caagatccag
    12961 cgtttcatcg aagagattca agatgcggta tccgacaact gaatgctgtt tcactttttc
    13021 tcgctcctcc tccgtaaaag ctttcagatg atctttcgac aggaggcttt ctttaagcac
    13081 gattttgccg atatcgtgca aataaccggc ccgcttaagc acgttgatct ccggcttgga
    13141 aagattaagg gcaattccga actctccgca caaccggctt acggctaagg aatgctgctt
    13201 ttctctcgga tacttcgaat gcaaagtctc gatgatcgta tcgatgatgt ccctgttcgc
    13261 cgatttgcga ttgatggtct tgtctctgta catcatattt tcagcgttgg ccatcgtctc
    13321 ttccagagac tgatagctgt tcgttttcgt atctatgccg agcgaaacgc tgcattttat
    13381 agcttcaata cgggcgttcg agaacccggt cttgatttgc gagagaattt tttcggcgtc
    13441 ccccatgtct gtcttcggca gaagtatcac gaattcatcg ccgccgacac gcgctatcac
    13501 gtcgcccttt ctgcaggatt gcctaagaat ctcggccgac ttctttataa gctcgtctcc
    13561 cgcggtgtga ccgaacacgt cgtttgtcat cttcaggccg ttgatatctg cgaagaccac
    13621 cgagagcggc agattatcgg gaatatctat tttatcgcgg ctgttctcga aacagcttct
    13681 gttgtgaaga cccgtcagta cgtcgtggca gctcagatat tcgatttccg cctctctttt
    13741 tttgcggtcg gtgatatcca taaaagtgat gactccaccg acgacctcgc cgtttcttat
    13801 ttgaggataa gaatggtact ccacatcgaa acaagtgccg tcggctctcc agaagacttc
    13861 gtcgtctgct tcgaatcctt tgccctgtct gatcgacatg aaaatcctgc actcgtcgat
    13921 aggaaaaggc cttccgtcac gatagctgtg atggatctgc agatgcatat tcttccccag
    13981 caattccgcg ggactgctgt atccaagcat tctgacgctg ctgatattgc agaaggtgca
    14041 tcttccgtcc atatcgattc cgtagatccc ttcggcagtg gaatccagaa tgagttgaag
    14101 ctgattttta ttttcttcca gcgccacatt tgcgccgtac agctgctccg ttcgctcttc
    14161 gacactctct tccagattgt ttatgagaaa ctgcatcttg tccgcgaccc tgttcaggct
    14221 ttccgatatg cttcctattt cgtcgtttct aacaacttcg gcgcgcctgg acaagtcccc
    14281 cgaggacaac gcggcggaga catcgagaag atcgtctata ggccttatga gtctgccgat
    14341 tacgatgtga taggcgataa gcgataccac cagtgcaaac gccacgagaa gggccgtcag
    14401 ataaacgctt tcaaccacac tgctcatgta aagcccttcg gggatcgcgg atatcaagat
    14461 ccagtcaatg cccggcatct gaatctcgcg aacattgaca aacagcttct ccctcccgct
    14521 gccgtagata aagattgagt ccccgctcgt tgcgtattga gcgtactctt gctgtatatc
    14581 acgatcttcc atttcactta tggtatttct tttcaacgtg ccgtcggaga gaacggagaa
    14641 gttgtcgacc cccgtcgagt tggcgatcag cgcgcccgtg tctttctcga aaagaatcgc
    14701 gtaaccgtca taattgttca cggtatctct gagaaaggct ccgacgcttg aaagaagcac
    14761 gtgcgttccc atcacgcccc gcaggttgcc atcgctatcg taaatgggcc acgctgcaga
    14821 aatggttagg tcgctcatca caaaatgttt atagatgggt gagaaggctg ggcctgcagc
    14881 ttcctgcacg gctttgtacc acgcgcgtgt ccgcggatcg aattttccgg ccactacgac
    14941 tcgctcgcct gccgtcaggt cttcgttcac cgaatagtac caggagttgc ccccggtaga
    15001 gacatcgttc ctcattattt cgataacgcc cttctcgttc ctgcgggctc cgtagtattc
    15061 gccgttttcc gtgccgtagc tgaaactgta tatctcctct tgttgagagc tcaaaacccc
    15121 gacgaagaac ttgtccctgg actcttcatc gtacaggtcg agaatgccgt tctctatgat
    15181 cttccggttg gcgtcgttga tatggtcagg ctcgtgcatg aaagagacga ctcgattgta
    15241 gatgccttcg ccaatagtct ccacgatgct cccggccgtc ttttcggccg aggacaacca
    15301 tccgctgaaa atcaggtatc cgatgccgcc gatagacaac agcatcgcca gtatgaacac
    15361 gattctaatt gcatttttaa tcgaaaaatc ctttctacta ttcatcccca tcttctctct
    15421 taaacacggc ccgcgccctg tctcttgact gaaaagagcg ttggcggccc tttttttgtc
    15481 tcttcgccag tcaagcaaaa ctatgccaac gttgactgcc agatctatct cgacagacct
    15541 ttgaaggggt gcgttatatg attttaaccg cgttccgggt tcgaaacgtg ggcaaagtgt
    15601 ttgactgcga ttttcataag gctcttagcg gtcatcgttg acgattatac gtcttgagga
    15661 attcgatcgc agcctcttca tccagaggtc tgctgatcag atacccctgt atattgtcgc
    15721 acccgtattc cgataaatac tctctctgct tttcgtgctc caccccttca gccacgacca
    15781 ggtgtcccag cttatgcgct atcgagacga tatcgccg
//
LOCUS       DAOOLY010000021        15688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3965_length_15688_cov_4.912493, whole genome shotgun sequence.
ACCESSION   DAOOLY010000021 DAOOLY010000000
VERSION     DAOOLY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_15688_cov_4.912493"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLD15_02110"
     CDS             complement(<1..279)
                     /locus_tag="PLD15_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPB62722.1"
                     /translation="MKIIVFGGTGRVGRVILDRALSDNHVVTIFVGNISKLTVRRENL
                     KIHAGDVFNSQAVRDAIRGQDAIISALGPDNSGSVNDTLAVAMKNIING"
     gene            489..860
                     /locus_tag="PLD15_02115"
     CDS             489..860
                     /locus_tag="PLD15_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62723.1"
                     /translation="MKKILIITIVVLMAGAIATAAGLGFRAQGIKQAQAGPGRCEAFV
                     DENGDGICDSYAVRPQPQDGTGKRWAVGRMNAGFKGNRGANRGTGLAGQENFVDENGD
                     GICDLLGTRKGLGKGNAFGRK"
     gene            1046..2878
                     /locus_tag="PLD15_02120"
     CDS             1046..2878
                     /locus_tag="PLD15_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB62724.1"
                     /translation="MKSNLSIIITIVFMFACVSLKASVLVFGGDGDYPPYEYIDENGD
                     LTGFNVDIMRAVARVLGMDLEIELGEWNYIVEKMKSGQLDGLLGMAVTAEREKLYSFS
                     IPHNYLYMSIFHRKNTPINSIEELHDKEIIVQKNGVMHDYLLENSITGKIITVEMPLD
                     GLKMLSSGKGDFGFFGKYQGLYLAKQNNLKNLVVFENTVFERDYCFAVSKDDQELLGL
                     LNKGLLFIRDSGEYREIYKKWFGTLDWFESNVQNIVFWGSVLIVLVLIALFVLSIWNS
                     ALKKRVDKRTEELTSRIAEYDGLQNKIRALHEVTLQMEKCSDEEEVYDLVVKAAREIL
                     KYDYYSLDIVEGDFLVAKRYSDTIEINSDVPKYEGIAGRTLKTGETILVDDITSVDYA
                     LPSSSKIRSLLSIPMGSFGVFQTVSLELAAFSREDVELAELLIYHAVGAIGIIRKTKE
                     ISFLAFHDSLTGLYNRTFFDEEVLRMDTSRNLPISLIFGDVNGLKIINDSYGHSYGDE
                     YLKAVAGTIKSSCRQEDLVVRWGGDEFVVLLFRTSEQHAREIIFRIESNLVAIRDFPV
                     TVSVSFGVSTKISEEQNIADLIEEAEFEMYNQKGLMKKAGKTMK"
     gene            complement(2975..3430)
                     /locus_tag="PLD15_02125"
     CDS             complement(2975..3430)
                     /locus_tag="PLD15_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB62725.1"
                     /translation="MWDSSARGVFVGLSASHNRLDLVRSVIEGVSMNLKWILDTIEES
                     IPIGNIRVIGGGVRSESWKRILSNILGKVLVVPRNVEEATSMGAAIIAGVGSGLFDFS
                     AARNFVVDVEQIEPARDEHEQYLRLYDLFKETYFSLRETFERLAGIRRG"
     gene            complement(3352..4500)
                     /locus_tag="PLD15_02130"
     CDS             complement(3352..4500)
                     /locus_tag="PLD15_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB62726.1"
                     /translation="MKYLLAHDLGTTGDKATLFNEKGILVCSSFEGYDTHYPAPGMVE
                     QNAEDWWKAFCKSTANIVKKASIDSPDVVALSISGQMQAAVPVDSSGRVLRRAMIWAD
                     SRSSRQVQKLLALIDEGEIYRITGSRVSPTYQGYKIAWLKENEPDLYEKASKFLQAKD
                     FINMRLTGEMVTDYSDAVMTNLFDINRLEWSKRLIDLNGIDQSKLPQTLPSVHDLGKI
                     RPEIASELGLSKNCRVILGAGDGCTAAVGAGAVGIGDTYLYLGSSSWISTITDSPLID
                     GKMRVFTGAHAVKGFYFPSGTMQAAGTSYSWIKDIAYSLESDNLKEKAGISSSTWTVS
                     CGNSIITARRSSTSLISTERGVLYGIPAQGAFLWDSQLPTTDWISFAR"
     gene            complement(4517..5179)
                     /locus_tag="PLD15_02135"
     CDS             complement(4517..5179)
                     /locus_tag="PLD15_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPB62727.1"
                     /translation="MKLFIDDSNTEAIAGLFELGIFSGISTNPSILKKSDRRPLEVVK
                     EMLDRFAGALFVQCASSEYEDTIKEGVEIFKLAPERIKLKIPFSRTGIKALGEFGKLK
                     IPVTITGIFSVPQVLMAAEAGADYVAPYASRMENLGIDLSVVESMQRVITNGAYSTKL
                     IAASFKTLTQISKMAELPIYGISLPVAMYGEFFAHSGTLKAIDNFDADWQSIEDRKWV
                     SI"
     gene            complement(5211..5912)
                     /gene="deoC"
                     /locus_tag="PLD15_02140"
     CDS             complement(5211..5912)
                     /gene="deoC"
                     /locus_tag="PLD15_02140"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPB62728.1"
                     /translation="MEKITGFSKRFDSTLLKANATYEEVDRFIDESVACDFRAVVVPW
                     YLMDRVIAKVSGTTVAAACGSGFPLGYDTTDVKEYMIKKSLELGPSMTDIDITANISA
                     IKSGDWGYFRREIEHLSSLLRNKTCKVIIEVSYLTTAEIEKACSILVEIPGVDYVKTG
                     TGYGSRATAIEDVETIHRIVRGLLGIKVSGGVKNLSHVENFIRAGADIFGSSNAIGIY
                     REFLEKYPGYNDLPL"
     gene            6196..7215
                     /locus_tag="PLD15_02145"
     CDS             6196..7215
                     /locus_tag="PLD15_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB62729.1"
                     /translation="MIPVSATLDDIARETGLSRATVARVLGGYGHSSKKARDKVLEVA
                     KKLNYKPNYIAKSMVTGETKNIGLIVGDIQNPFFSTIARAISDVIVPEGYSLIVSSTD
                     EKLEVEKTSIDKFFQKQVDGLILAPVSRSNSGHLHELVKSKIPVVLIDRVIDGLDLDM
                     IVSEDVGGGQVATEYLVGLGHRNIGFLSDNLDISTNYDRLSGYRKALRENNIRERECW
                     IKLGGFRVDGAYKSGVALLSQNKDLTAVIASNNYMAAGLLLAAKDMGLSIPRDLSVIS
                     FDDIVWFELTKPPITAVAQNTVEMGSLAARSVLTALRDQRSVKGITRLPTKLIVRESC
                     AKLQN"
     gene            7241..8305
                     /locus_tag="PLD15_02150"
     CDS             7241..8305
                     /locus_tag="PLD15_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB62730.1"
                     /translation="MFRLTLDNFLEKAKKSVLFTSPSLEKDLTEFLEEHTDRIKKLAS
                     MTLEKDFKQIYWVGSGNSWCNLYSGDYILNKMTDLPSAYYKSYDFIWTNPARLGEDSL
                     VILASFSGNTEDTAAALRFANEKGATTISFTSKPDSILAREADEAIVYNSNALFILPL
                     AGAFVFALEIARLQGRDVSKLFEQLQTMPRLLGKIYKEEENRAARLASKYRDYDLFYV
                     LSSASAYAIGYKFGLTVFMENMRVNASFMETSEFRHGPAEMLDGHKPLLLFLVGTDES
                     RDMSERVIKIAQSNGSEIMRFDAKDYGDFDPLFAPFLLMVPLQWFAVYSAYYRGIFDL
                     DERVLMGRGKMSTGSQITWP"
     gene            8330..9439
                     /gene="nagA"
                     /locus_tag="PLD15_02155"
     CDS             8330..9439
                     /gene="nagA"
                     /locus_tag="PLD15_02155"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPB62731.1"
                     /translation="MIFENVLVVDPIDGEFVGSVETTRGVISGVKRSIKNVDFEYILM
                     PGFADPHTHGSAGVDALSMDLRGLKKWESFLYSQGVTYFLPTTMSSTPGAILSSAKVV
                     REYLENNGSTSVGGIHYEGPYISVKRKGAQNPSLIRKIDLGEIESTLIETVRLITMAP
                     ELEGFIEASKLITDRGIPISLGHTDASYEDMKRAFEAGCTRVTHFPNGMNTLHHRELG
                     CIGAVFSMPFSVEMIIDGVHSLPEFVKMVYDIKGPERIMIVTDTIDAAGMPDGEYELG
                     GLKVILKNGRPTLEDGTIAATVLMFNQAVQNFKSFTGCTLKDLARVSSYNSLRLLGIE
                     DRGRIAGGYLANLVLLDRNLKVVETVLNGKTAFKA"
     gene            9750..10718
                     /locus_tag="PLD15_02160"
     CDS             9750..10718
                     /locus_tag="PLD15_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62732.1"
                     /translation="MRKVLVLLAMASILLGATLGFAVYNIAFIVKATDSDFWQYTIVG
                     GKNAEHDLQGLAKVTVYGPPSEADIDLQVSILENVVNTKPDAIVISSTSSEAPSLILD
                     AAYKQGIKIVLIDNFVYDTGYNSFLATNNAVGGAHAADKLVEALKANGTALKGKVGLI
                     SAMAGVQVLTDRDAGFTNRLKEIAPDLQVLPTIYTNNDISRSANAAEDLLIAHSDLVG
                     FFADNNHTGIGVARVIEQRGLENKIMAVAYDSDPLEIEALDSGALKALIVQDPHGMGY
                     KGVMFAFMAINGEPLPAYLDTGVYVITKDILEDSMMILDPFSRKKY"
     gene            10806..11501
                     /gene="deoC"
                     /locus_tag="PLD15_02165"
     CDS             10806..11501
                     /gene="deoC"
                     /locus_tag="PLD15_02165"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPB62733.1"
                     /translation="MKKDISFNRRFDNTLLKPESTWEEIVSFVDETVSYNFRNVVVPW
                     YAIPSLVIEKVKGTEVGINVGPGGFPFGMVPTEMKMREIKYYLSLGETVTDFDIVINV
                     SAVKSCNWDYLKREFVVLSEPVKKENRICKFIIETSRLTEEEIVKVCELLADVPTIDF
                     VKTGTGFGPRATSYRDVELINSVVSGKKEIKVSGGVRTLEQVEKFMELGATVFGSSAS
                     VSILKEYRKKYGT"
     gene            11516..13039
                     /locus_tag="PLD15_02170"
     CDS             11516..13039
                     /locus_tag="PLD15_02170"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB62734.1"
                     /translation="MPEARTVLELENIYKTFPAVKALDNVSMSVKSGEVRGLVGENGA
                     GKSTLIKIITGAYTKDSGSMLFEGNDIGRNSPLISKGLGIYAVYQDVMVTPDLTVAEN
                     FFLGHQPRIGPFINWKKMYRESEEFLDEIGLEINVHKNIKELSIAESEMVAICKALWQ
                     KPKLVIFDEPTAVLTRSETKILFKIIEDLKKRGTAIIYISHNLEEVFEVCETVSVLKD
                     GATVGTYTTEELGNVDRLIPLMVGRSIEEMYHKTATETGQELLRVENLSGNRFENISF
                     SVKSGEVVGLFGLVGAGRTEIARAIFGVDHFVEGKIVVKGSEAAIKKPKDALDNGIGY
                     LPEDRRKQGIFLQQDIDFNLNIINFDKAMRAGIINYGKAHAQTRDYVEKLSIKIGSIY
                     QPISQLSGGNQQKVIIARWLCKNPDVLIFDEPTVGIDVGTKSEIYRLFGDILNSGKGI
                     ILISSYLPELMGVSDRIYVISNGKMAAEVQKKDFSEEYLLTLAMKNLVSKEKIKEAV"
     gene            13041..14033
                     /locus_tag="PLD15_02175"
     CDS             13041..14033
                     /locus_tag="PLD15_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62735.1"
                     /translation="MAGKRERKAFAERNLLIILVVMWVFLAIATKGNFSSWANITNLI
                     RQSSINGVVAIGMTLIIITGGIDLSVGSIVGLSGMIYAIMTSTRGDFKLSSFWGIIIA
                     LAVGALVGLINAVAIHDGKVPAFIATLGMMTLVRGIVMYVSSGRMVTGVPTEYRQFAV
                     DEFLGIPALAWTWIILALVMIFVLKYTKFGRNLYAIGSNEEAARLSGINIRFNMYAFY
                     IVAALFSGVAGLMLGTRMAAGVPTGGQGYELDAIASVVIGGASLSGGVGTILGTALGA
                     LIIQTIRNGGNLLGVDPFIMQIIIGAIIILAVFFDQFIKSRKKGEGLKKLFKRA"
     gene            14040..14441
                     /locus_tag="PLD15_02180"
     CDS             14040..14441
                     /locus_tag="PLD15_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB62736.1"
                     /translation="MKISKFFHVAIEVPDLDKAIEFYTGLLSLSLINREKLPEKKLEV
                     AFVAGEGCEIELMCYEDSKDRQFAPENQSHFQHLSFLVNNIDTAMTYLKEKGIELESE
                     EPIPVFNGRVCYNTFKGPGGELLEIAQIMKR"
     gene            14472..15404
                     /locus_tag="PLD15_02185"
     CDS             14472..15404
                     /locus_tag="PLD15_02185"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPB62737.1"
                     /translation="MNRVTVFGSYVMDLTTLSPHIPVVGETVFSGPFKMGPGGKGFNQ
                     AVAARKSGSQVQFMTKIGRDLFAPFVREAFDRFDISRDNLLESVESGTGVALIIVDRN
                     NGNNAIAVAPEACNELSVEDVKERKEIFESCDVFLTQFEANLEATYEAIKIAKGSGAK
                     VLLNPAPVKEFDHAILKYVDVIMPNEIEASLMTDIPLDGLDSIKDIARELGKHVETVV
                     LTLGGKGVYCPSVSDDLIPACKVATIDTTGAGDAFAGIFASYMSRGISIAGAIDYARV
                     GAAISTTRFGTSPSMPEKEEIEALLSEIKGGASI"
     gene            15401..>15688
                     /locus_tag="PLD15_02190"
     CDS             15401..>15688
                     /locus_tag="PLD15_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB62738.1"
                     /translation="MNYVIAYDLGTTGNKATLYSIDGKLQASAFSSYRTFYPGPNQVE
                     QDPEEWWESVKITTSELLSKAGIDPREIKAVSFSGQMMGAIPIDRNGKTLRR"
BASE COUNT         4209 a         3727 c         3937 g         3815 t
ORIGIN      
        1 gccgttgatt atgttcttca tcgccaccgc cagcgtatcg ttcacgcttc cggagttatc
       61 gggaccgagt gcgcttatta tggcgtcctg ccccctaatc gcatccctca cggcctggct
      121 gttaaaaacg tctcctgcat gtattttcag attttctctc ctcactgtga gcttggagat
      181 gtttccaaca aatatcgtca caacgtgatt atcgctgaga gctctgtcca ggatcaccct
      241 cccaactctg ccggttccac caaacactat tatcttcaag gctgttaccc cctttcattc
      301 ctacacttat atattataga ctcaactcga aaaatccaaa taaccgttat cgtacaatac
      361 agcctgaacg gacagttttg cgctgacaat ttcgaaaaac cgcgattatt tgcccggagc
      421 tctaagaaaa ctctaaggat tcgatcatag acttgatacg taagaaaatt cactgggagg
      481 tgtgaaagat gaaaaagata ctgattatca cgatagttgt gcttatggca ggagcaattg
      541 caacggcggc gggattggga ttcagagccc agggcattaa gcaggctcag gccggcccgg
      601 gtagatgcga agccttcgtt gatgagaacg gcgatggaat ctgtgactca tacgcagtga
      661 gacctcagcc gcaggatgga acgggcaaga gatgggctgt cggaagaatg aacgccggtt
      721 tcaaaggcaa cagaggtgca aatagaggaa ccggactggc tggccaggag aatttcgttg
      781 atgagaacgg cgacggaata tgcgatcttc tgggtaccag aaaaggtttg ggtaaaggta
      841 acgcgttcgg cagaaagtaa tcccgccatt gaatgtgcag agagtccctt agggggctct
      901 ttctttttat cgccccacac aaacatctcc catatgaaga tagaattaac tggcatgtcg
      961 gtgattcggt atttcttcgt cacactagag ccgtttttgt caaggtacgt aagatatgct
     1021 aagataatac taccgggggt aaatgatgaa atcaaatctt tcaatcatta taacgatagt
     1081 atttatgttc gcctgcgttt ctttaaaggc aagtgtactc gtcttcggtg gcgacggaga
     1141 ttaccctcca tacgagtata tcgacgaaaa cggcgactta acgggtttca acgtcgatat
     1201 tatgagagcg gttgccagag ttctgggaat ggatttagaa atagagctcg gcgaatggaa
     1261 ctacatagtc gagaagatga agtccggcca gctagacggt ttgctgggca tggcggtaac
     1321 ggccgaacgc gagaagctgt actccttttc gatcccccac aattatcttt acatgagtat
     1381 ctttcacagg aaaaacaccc cgataaactc gatcgaggag ctgcacgata aagagataat
     1441 cgttcaaaag aacggcgtta tgcacgacta ccttctggaa aactcaatca ctggcaaaat
     1501 catcaccgtc gaaatgccgc tcgacggatt gaaaatgctt tcctcaggca agggcgattt
     1561 cggatttttc ggcaagtatc aggggcttta tcttgccaaa cagaacaacc tgaaaaacct
     1621 ggttgttttc gaaaacaccg tcttcgagag agactactgt ttcgccgttt cgaaagacga
     1681 ccaggagctc ctcggcctac tcaacaaggg gcttctcttc ataagggaca gcggcgagta
     1741 cagggaaatc tataaaaaat ggttcggcac gctcgactgg tttgaaagca acgttcaaaa
     1801 tatcgtattc tggggttcgg tcctcatcgt gcttgtgtta attgctctat ttgtgctgtc
     1861 gatatggaat tcggctctaa agaagcgcgt tgataagaga accgaagaac tgaccagtag
     1921 aatcgccgag tacgacggtc tccagaacaa gatacgcgcc ttgcacgaag ttacgcttca
     1981 gatggaaaag tgcagcgatg aagaagaggt ttacgatctg gtggtgaagg ccgcaagaga
     2041 aatccttaaa tacgattatt acagtctcga catcgtcgaa ggtgatttcc tggtggccaa
     2101 aagatactcc gatactatag agatcaactc ggatgtgcca aaatacgaag gaatcgccgg
     2161 tagaaccttg aagacgggag agactatact cgtcgacgat atcacctcag tcgattacgc
     2221 gctcccctcc agcagtaaga tcaggtctct gctcagcatc cccatgggct cattcggcgt
     2281 cttccagaca gtttctctgg aactggccgc cttcagtagg gaagatgtgg aactggcgga
     2341 gttgctcatc tatcacgcgg tcggtgcgat cggcatcata cgcaaaacga aagagataag
     2401 cttcctggcc ttccacgatt cgctgacggg gctttacaac cgcacatttt tcgacgaaga
     2461 ggttttgcgg atggacacct ctagaaacct gccaatatcg ctgatattcg gcgatgtgaa
     2521 cggtttgaag atcataaacg atagttacgg ccactcttac ggcgacgagt atctcaaggc
     2581 cgttgctgga acgataaaaa gctcatgcag acaggaagat cttgttgtgc gctggggtgg
     2641 agacgagttc gtagttttac ttttcagaac ttcggagcag catgccaggg aaatcatctt
     2701 ccgcatcgag agcaacctgg ttgccataag agacttcccc gtaaccgtga gcgtttcttt
     2761 cggtgtttcc acgaagatct ccgaagagca gaatatcgcc gatttgatcg aagaagccga
     2821 attcgagatg tacaaccaga aaggactaat gaaaaaagcc ggcaaaacca tgaaatgaag
     2881 aaaaagaaag acccggcaac cacggatcgg ctgactactt cgacgatcta cagggccggg
     2941 cctctggtgt ggtacaactc ccgtttagtg agcgctagcc ccttctgatt cctgcaagcc
     3001 tctcaaaggt ctccctgagc gaaaaatagg tctctttgaa gagatcgtac agtctcagat
     3061 attgctcgtg ctcgtctctc gccggctcga tttgctcgac atctactacg aaattgcgag
     3121 cggcggagaa atcgaaaagg ccggaaccta ctccggcaat tatcgcggca cccatcgatg
     3181 tcgcttcttc aacgtttcgt ggaactacca gtacttttcc tagaatgtta gagagtatcc
     3241 tcttccagct ctcgcttctg acaccaccgc ctatgacacg gatatttcct atcggtatcg
     3301 actcttcgat ggtgtccagt atccatttta gattcatcga gacgccctcg atcaccgagc
     3361 gaacgagatc cagtctgttg tgggaagctg agagtcccac aaaaacgccc cttgcgctgg
     3421 aatcccatat aggactcctc tctccgtgga gataagggag gtagatgatc tcctcgccgt
     3481 gatgattgaa ttgccgcagg agaccgtcca ggtacttgaa gatatccccg ccttttcttt
     3541 gagattatcg ctttcaagac tgtatgctat atcttttatc cagctgtaag aagtaccggc
     3601 cgcctgcatc gtgccggatg gaaaatagaa acccttcacg gcgtgcgcac cggtgaagac
     3661 tcgcattttc ccatctatga gaggactgtc cgttatggtg gatatccacg aagaagagcc
     3721 gaggtagaga taagtgtcgc cgattccaac cgcaccggct cccaccgcgg cggtgcaacc
     3781 gtcgccggcg ccgagtatca ccctgcagtt cttcgagagc ccgagttcgg aggctatttc
     3841 tggccttatc tttcccagat cgtgaaccga cgggagtgtc tgtggaagct ttgactgatc
     3901 tattccattg aggtcgatca gtcgcttgga ccactcgagc ctatttatat cgaagaggtt
     3961 cgtcatcacg gcgtcagagt agtcggtgac catctctccg gtgagacgca tgtttataaa
     4021 atccttggcc tggagaaact tgctagcctt ttcgtaaagg tccggctcgt tctccttgag
     4081 ccaggcgatc ttgtacccct ggtaagtcgg tgaaactctc gaacctgtta tcctgtagat
     4141 ctctccttca tcaatcaaag caagcagctt ttggacctgt ctggagcttc tagaatctgc
     4201 ccatatcatc gcgcgtctca acactcttcc agagctgtct acgggaaccg cggcctgcat
     4261 ctggccgctt atgctgaggg cgaccacatc cggggagtcg atcgaagcct tcttaacgat
     4321 attcgctgtg cttttgcaga aagccttcca ccagtcttcg gcgttctgct cgaccattcc
     4381 aggagccggg taatgggtat cgtatccctc gaaggaagag cagacgagta ttcccttttc
     4441 attgaaaagc gtcgctttgt ctccggttgt accaagatcg tgagccagta aatacttcat
     4501 acattctccc ggcgggttat atggaaaccc attttctatc ttctattgat tgccagtcgg
     4561 catcgaaatt gtctatcgct ttaagagtgc cgctgtgagc gaagaactct ccgtacatgg
     4621 caacaggaag agagataccg taaatcggta gttctgccat ctttgaaatc tgcgtcaatg
     4681 tcttgaaact ggccgcgatc aatttggtag agtaagcccc gttggttatc actctctgca
     4741 tactctcgac aacagaaaga tctataccca aattctccat gcgactggcg taaggagcca
     4801 cgtaatcggc tcctgcctcg gcggccatca agacctgggg caccgagaag attcccgtga
     4861 tcgtcaccgg gatcttcaac tttccgaact ctccaagtgc ctttatacct gttctggaga
     4921 agggtatttt caacttaatt ctttcggggg cgagtttgaa gatctcgacg ccctctttta
     4981 ttgtgtcttc gtattccgaa gaggcacact gaacgaagag cgctcctgcg aatctatcca
     5041 gcatttcttt gacaacctct aaaggccttc tatcgctctt tttgaggatg ctcgggttcg
     5101 tactgattcc cgaaaagatg ccgagttcga aaagccctgc gatcgcttcg gtgttgctat
     5161 catcgataaa aagcttcata aaaacgcacc tcctttgcga atgacttgat ctataaaggc
     5221 agatcgttgt agccgggata tttctccaag aactctctat agatgcctat ggcgttgctg
     5281 gaaccgaaaa tatcggctcc ggcgcgaatg aaattctcta catgcgagag gtttttgaca
     5341 cctccggaaa cctttatccc cagtaagccc ctgacaattc tgtgtatagt ttcgacatct
     5401 tcgatcgctg tggcccttga accgtatccc gttccggtct tcacataatc tactccgggt
     5461 atttcgacca gtatggaaca ggccttctcg atctcggccg tcgtcagata ggagacttct
     5521 atgatcactt tgcaagtttt atttcgaaga aggcttgaga gatgctctat ctccctcctg
     5581 aaataccccc agtcacccga tttgatggcc gaaatgttgg ccgtgatatc tatatcggtc
     5641 atcgacggcc caagctccag cgatttcttt atcatatatt cctttacatc ggtcgtatcg
     5701 tacccgagcg gaaagcccga tccacatgct gcggcaacgg tcgttcccga aaccttcgcg
     5761 atcactctgt ccattagata ccagggaacc acaaccgccc ggaaatcaca ggccacgctc
     5821 tcgtcgatga atctatcaac ttcctcgtag gtggcgttgg ctttgagtaa cgtgctgtcg
     5881 aatcttttgg aaaaaccggt tatcttctcc acggctacct ccacaatgtg aaatacaggg
     5941 aaagccattc aacacaaggt tccaattttc gtgaaatatt gagagcggta tcaatatggc
     6001 tttctaattt attataacct tttttgtttt aaatgtctat attctctttc tatacaggca
     6061 ttattgtaga tatttgatac ttgacacacc atgcgaaaat aagatagaat atctccgata
     6121 cattgatacc gatatcatta ctagtatcac agctcttcaa aattgagatg tggtatatcg
     6181 gtgaaaggag ggaggatgat acccgtgagt gcgacactcg acgatattgc aagagagacc
     6241 ggtctatcca gagccacggt ggcacgtgtg ctcggagggt acggacattc cagcaaaaaa
     6301 gcgagagaca aggttcttga agtagccaaa aagttgaatt acaaaccgaa ttacatcgca
     6361 aagagcatgg taacgggtga aacgaagaat atcggcctga tagtcggcga tatacagaac
     6421 cccttctttt ctaccatcgc cagagccata agcgatgtga tcgtaccaga aggatacagc
     6481 cttatcgtat caagcaccga cgagaaactg gaagtcgaaa agacttcgat agataaattt
     6541 ttccagaaac aggttgatgg actgatactg gccccggttt cgaggagcaa cagcggccat
     6601 cttcatgagt tggtcaagtc gaagattcca gtagttctca tagacagggt gatagacgga
     6661 ctggatctcg atatgatcgt tagtgaagac gtaggtggcg gtcaggtcgc gactgagtac
     6721 cttgtcggtc tgggtcaccg caatatcggt tttctttccg acaatctcga tataagtaca
     6781 aactacgata ggctctcggg ttatcggaag gcactaaggg aaaacaacat ccgtgagaga
     6841 gagtgctgga tcaaactcgg aggcttcagg gtagatggag cgtacaagag cggagtggcc
     6901 cttctgagcc agaacaaaga cctaacggca gtaatagcct ctaacaacta catggccgcg
     6961 ggactacttc tggcagccaa ggatatggga ctctcgattc cccgcgatct ctccgtgata
     7021 agtttcgacg acattgtctg gttcgaactt accaaacctc cgataactgc tgtcgcccag
     7081 aacactgtag agatgggaag ccttgcggcc agaagtgttt tgacggctct tcgagaccag
     7141 agaagcgtga aggggatcac cagactgcct acaaaactta tagttcgcga atcctgtgcc
     7201 aaacttcaaa actaggatag atttccatat ataaacggag gtgttcagat tgactttgga
     7261 taatttcctg gaaaaagcaa agaagagtgt tctctttacc tctccttctc tagaaaagga
     7321 cctgacggag tttctagagg aacatacgga tagaatcaaa aaattggcat ccatgactct
     7381 ggaaaaagat ttcaagcaga tctactgggt cggtagcggc aactcctggt gcaaccttta
     7441 ctcgggagac tacatactca acaaaatgac cgatcttccc tcggcttatt acaaaagcta
     7501 cgacttcatc tggaccaatc ctgcaagact gggcgaagac tcgctggtta ttctggcctc
     7561 attctccgga aacaccgaag acacggctgc ggcgttgcgg ttcgccaacg aaaagggagc
     7621 caccaccatt tctttcacaa gcaagcccga tagtattctg gccagagagg ccgatgaggc
     7681 aattgtatac aactcgaacg ccctgttcat tctccccctc gccggagcgt tcgtattcgc
     7741 tctcgaaata gcgagattgc agggaaggga cgtatcgaaa ctgttcgagc agcttcagac
     7801 tatgccgcgt cttctcggaa agatctacaa ggaagaggaa aacagagccg cgagacttgc
     7861 aagcaagtac agagattacg atctcttcta cgttctttcc agcgcttcgg cctacgcgat
     7921 cggttacaag ttcggactca cagtcttcat ggaaaacatg agagtgaacg catctttcat
     7981 ggaaacctcc gagttcagac acggtccggc cgaaatgctc gacggtcaca agccgctgct
     8041 tctcttcctc gtagggacag atgaatctag agatatgagc gaacgcgtta taaaaatagc
     8101 ccagtccaac ggtagcgaga taatgaggtt cgatgcgaag gattacggag atttcgatcc
     8161 actcttcgcc ccgttcctct tgatggttcc acttcagtgg ttcgccgtct attcggctta
     8221 ttacagaggt atcttcgatc tagacgagag agttctcatg ggaaggggaa agatgtccac
     8281 tggaagtcag attacctggc catgatatgc tgatgggagg agagagccta tgatttttga
     8341 aaatgtcctg gttgttgatc cgatagatgg agagttcgtt ggaagcgtag agactaccag
     8401 aggagtgatc tccggggtaa agagaagtat aaagaacgtc gatttcgaat atatactgat
     8461 gccgggattc gccgatcctc acacacacgg ctcggccggt gtagatgcgc tttctatgga
     8521 tctaagggga ctcaaaaaat gggagagctt tctctattct cagggtgtca cttacttcct
     8581 gccgacaacc atgtcttcga ctcccggagc aatactctcc tccgcaaaag ttgtcagaga
     8641 gtacctcgaa aacaacggct caacatctgt cgggggtatt cactacgaag gtccatacat
     8701 aagcgtcaag aggaaaggtg cccagaaccc ttccctgata aggaagatcg acctaggcga
     8761 gattgagtcc acactgatag aaacggtaag attgatcacc atggctcccg agttggaagg
     8821 ctttatcgag gcctcgaagc tgataaccga tcgagggata ccgatctctc tgggccacac
     8881 cgatgcatcc tacgaagata tgaagcgagc ctttgaagct ggttgtacga gagttacaca
     8941 ctttccaaac ggtatgaata cgcttcatca cagggagctc gggtgtatcg gagcggtctt
     9001 ttcgatgcct ttctccgtgg aaatgataat cgacggggta cactccctcc ccgagttcgt
     9061 gaagatggtc tacgacatca aaggtccgga aaggatcatg atagtcaccg atacaataga
     9121 cgcggccgga atgcccgacg gcgagtatga actgggcggc ttgaaagtga tcctgaaaaa
     9181 cggcagaccc acgctcgaag atggaacgat agccgcaacg gtcttgatgt tcaatcaggc
     9241 ggtacagaat ttcaaaagct tcaccggctg tactctgaag gatctcgcac gtgtctcttc
     9301 ctacaactct ctccggttgt tgggcataga ggaccggggc agaatcgcgg gaggatatct
     9361 cgccaacctg gtcttgctcg acagaaatct gaaagttgta gagaccgtgt tgaacggaaa
     9421 gacagcattc aaagcttgaa gcggaagtct agagacttcc gctttttgtt ttctcacaac
     9481 agacaatatc cagaaaccgt gtagaaggat attcttttcg tatagtgttt atagactttc
     9541 gtaaatccca agtaaatttg ttgattatta agattgaaga gtgattatgt tcgtttattc
     9601 gggtttattg tcaaatatct tcttcttcct ttctttttat acaacttcta aatttgtacc
     9661 ggctacagcg ttctgatata atcaagatgt ttgtacgtac attggtacat acgtatgtac
     9721 agaactacac tagaaggagg gttttgctaa tgcgtaaagt actcgtacta cttgctatgg
     9781 cctcgattct tctcggagcc accttaggtt ttgcagttta caacattgcc ttcatagtca
     9841 aggcaaccga ttcggatttc tggcagtaca cgatagttgg aggtaagaac gccgaacacg
     9901 acctccaggg actcgcgaaa gtaaccgtct acggaccccc atcggaagcc gatatcgatc
     9961 ttcaggtttc tatactcgag aacgtagtca acacgaaacc cgatgcgata gtcatctctt
    10021 ccaccagttc cgaagctccg tccctgatcc tggatgccgc ctataagcag ggcataaaga
    10081 tcgtccttat cgacaatttc gtttacgaca caggttacaa ctccttcctt gcgaccaaca
    10141 acgcggttgg cggcgcacac gcggctgaca aactggtcga ggcactcaag gccaacggta
    10201 ccgccctcaa gggcaaggta ggactcatca gcgctatggc cggtgttcag gttctgaccg
    10261 atagagacgc cggtttcacc aacaggttga aggaaatcgc accagatctc caggttctgc
    10321 cgactattta caccaacaac gatatatctc gttccgcgaa tgccgctgaa gaccttctca
    10381 tagcccattc cgatctggta ggtttcttcg ccgacaacaa ccacaccggt atcggtgtcg
    10441 cgagagtcat cgaacagcgc ggtcttgaaa acaagataat ggcagtcgct tacgactccg
    10501 atcctctgga aatcgaagca ctcgatagcg gcgcccttaa ggccctcatc gttcaggacc
    10561 cccacggtat gggatacaag ggagttatgt tcgcattcat ggcgataaac ggtgaaccgc
    10621 ttccagccta tctcgacacc ggagtttatg taataaccaa ggatattctc gaagattcga
    10681 tgatgatcct agacccgttc tcgagaaaga aatattgata aattcacggt tctagcaagg
    10741 actgaatggg cagcgcaagc tgcccatttt cagaattttt ggtcacacaa ttccggaggt
    10801 agtgtatgaa gaaagatatt tctttcaaca gacgattcga caacacgtta ttgaaacccg
    10861 agtcaacctg ggaggaaata gtgagtttcg tcgatgagac tgtttcatat aatttcagaa
    10921 acgtggttgt cccatggtac gcgattccct cgctggtaat cgagaaagtt aaaggcacgg
    10981 aggtcggcat aaacgtaggt cccggaggct ttcccttcgg catggttccg acagagatga
    11041 agatgagaga gattaagtac tatctttctc ttggcgagac ggtgaccgat ttcgatatag
    11101 tgatcaatgt ctccgccgtc aagtcctgca actgggacta tctcaagaga gagttcgtgg
    11161 tactgtctga acctgtcaag aaggaaaaca gaatctgcaa gtttatcata gagacgagca
    11221 gactgaccga agaggagata gttaaggttt gtgaactgct ggcagatgtg ccaacgatag
    11281 atttcgtgaa gaccggcacc ggcttcggcc cgagggcgac atcctatcga gatgtagaat
    11341 tgatcaactc cgttgtgtcc ggaaaaaaag agataaaggt atccggtgga gtgagaactc
    11401 tcgagcaggt tgagaagttc atggagcttg gcgcgacggt ttttggatcg agtgccagtg
    11461 tttctatact gaaagagtac cggaagaaat acggaaccta aaggagagcg atcacatgcc
    11521 agaagcaaga accgttttag agcttgagaa catctacaag acgttccccg ctgtgaaggc
    11581 gctcgataac gtaagcatgt cggtaaagag cggggaagta aggggtcttg tcggagaaaa
    11641 cggagccggt aagtcaactc ttataaagat aattacgggc gcttacacca aagactccgg
    11701 atcgatgctt tttgaaggaa acgatatcgg tagaaactct ccgctcattt ccaaaggcct
    11761 cgggatctac gcggtttatc aggatgtcat ggttacaccc gacctcacag tcgcagagaa
    11821 cttctttctc ggccaccagc ctagaatagg tcctttcatt aactggaaga agatgtacag
    11881 agagtccgag gagtttctag atgaaattgg tctggaaatc aatgtccaca aaaacataaa
    11941 ggaactcagt atcgcagaga gcgagatggt agccatctgc aaggctctct ggcagaaacc
    12001 gaaactggtc attttcgacg aacctacggc cgttctcacc aggagcgaga cgaagatact
    12061 cttcaaaata atcgaggatc tgaagaagcg tggaacagct atcatataca tctcgcacaa
    12121 cctggaagag gttttcgaag tctgtgaaac cgtctccgta ctcaaggatg gagcgacggt
    12181 cggtacttac acaaccgaag aacttggaaa cgtggacaga ctgattcctc tcatggtcgg
    12241 aagatctata gaggaaatgt accataaaac ggctaccgaa accgggcagg agttgttgag
    12301 agttgaaaac ctctcgggaa acaggttcga gaatatctct ttcagcgtaa aatccggaga
    12361 ggtggtcgga ctgttcgggc tggtcggggc tggaagaacc gaaatcgcca gggcgatttt
    12421 tggagtcgac catttcgttg agggcaagat agtggtcaaa ggtagcgagg ccgcaatcaa
    12481 gaaaccgaaa gacgccttag acaacggaat aggctatctg cccgaggacc ggaggaagca
    12541 gggtatattc cttcaacagg atatcgactt caatctgaat atcataaact tcgacaaagc
    12601 catgcgtgcc ggtataataa attacggcaa agcgcatgcc cagaccagag attatgtcga
    12661 aaagctttcg ataaagatcg gcagtatcta tcagcccatc tcccagctga gcggtgggaa
    12721 ccagcagaaa gttatcatcg ccagatggct ctgcaaaaat cccgacgtac tgatattcga
    12781 cgagccgact gtcggtattg acgtgggaac caagtcggaa atctaccgcc tctttggaga
    12841 tatattgaac agcggcaaag ggataattct gatttcctcg taccttcccg agctcatggg
    12901 agtatccgac aggatatatg ttatctccaa cggaaagatg gccgccgagg ttcagaagaa
    12961 ggacttttcg gaagagtatc tcttaacgct cgcgatgaag aatctagttt cgaaggaaaa
    13021 gataaaggag gcagtttgaa atggctggga agagggaaag aaaagcattt gcggaaagaa
    13081 acctgttgat tattctcgtt gtgatgtggg tctttctggc catcgcaaca aagggaaact
    13141 tctcctcgtg ggccaatatc accaacttga taaggcagtc ctctataaac ggagttgtgg
    13201 ccattggaat gactttgatc atcataaccg gcggcataga tctgtcggtg ggctctatcg
    13261 ttggcctgtc gggaatgatt tacgccatca tgacctctac cagaggagac ttcaaacttt
    13321 cctctttctg gggaataatc atagcccttg ccgtgggcgc gctggtgggt ctgatcaatg
    13381 cggttgccat tcatgatggt aaagtcccgg ctttcattgc gacactggga atgatgacgc
    13441 tggtcagggg tatcgtaatg tacgtctcca gcggcaggat ggttacggga gtcccgacag
    13501 aatacagaca gttcgcagta gatgaattcc tgggtatccc agctctcgcc tggacctgga
    13561 taatactggc tttagtcatg atattcgtcc taaaatacac caagtttggc cgcaaccttt
    13621 acgcgatcgg cagcaacgag gaggctgcga gactttccgg tatcaatatc agattcaaca
    13681 tgtacgcatt ttacattgta gccgcccttt tcagcggcgt cgcgggattg atgctcggga
    13741 ctagaatggc cgccggggtg ccaacgggag gacagggcta cgagctcgac gccatagcct
    13801 cggtcgttat cggtggagcc agccttagcg ggggcgtcgg cacgatactc ggaacggctc
    13861 tcggcgcact catcatccag actataagga acggcggaaa cctgctcggt gtcgatccgt
    13921 tcataatgca gatcataata ggtgcgataa tcattctggc cgtcttcttc gatcaattca
    13981 taaagagcag gaagaaggga gagggcctga aaaaactctt caagagggcg tgattgatga
    14041 tgaagataag caaattcttt catgttgcca tcgaagttcc agatctagac aaggcaatcg
    14101 aattctacac aggactgctc agcctgagtc tgatcaaccg tgaaaagctg ccggaaaaga
    14161 agctggaagt ggctttcgtt gctggagaag gttgcgagat cgagctgatg tgttacgaag
    14221 acagtaaaga cagacagttc gctccagaga atcagtcgca ttttcagcac ctctcttttc
    14281 ttgtcaacaa catagacacg gcgatgacct accttaagga aaagggaatt gagctcgaat
    14341 cggaagaacc gataccggtc ttcaacggga gggtatgtta caacactttc aaaggccccg
    14401 gcggagaact tctggagatc gcgcagataa tgaaaagata atccgtatcc tggatagtct
    14461 ggaggatttg tatgaacaga gttaccgttt ttggaagcta cgtaatggat ctgaccacac
    14521 tctctccaca cattccggtg gttggagaga cagtcttctc cggccctttc aagatgggtc
    14581 cgggcggtaa aggcttcaat caggccgtgg cggctagaaa gtccggttcc caggtacagt
    14641 tcatgacgaa aataggaaga gatcttttcg ctcccttcgt cagggaagcc ttcgatagat
    14701 tcgatatatc cagagataat ctcctcgagt ctgtcgaatc cggcaccgga gtcgctctga
    14761 tcatagtgga ccggaacaac ggaaacaacg ccatcgcagt cgctcccgaa gcctgcaacg
    14821 aactgagcgt tgaggatgtg aaggaacgga aagagatatt cgaaagctgt gatgtctttt
    14881 taacacagtt cgaggctaat cttgaagcta cctacgaagc tataaagatc gccaaaggct
    14941 cgggcgcgaa agtactgtta aaccctgcgc cggttaagga attcgaccac gcgattctaa
    15001 aatacgtaga tgtaatcatg cccaacgaaa tcgaggccag tttgatgacc gacatccctc
    15061 tggatgggct cgattcgata aaagatatcg cccgggaact ggggaagcat gtggagacgg
    15121 tggtgttgac actcggggga aaaggtgttt actgcccttc tgtcagcgat gatctgatcc
    15181 cggcatgcaa ggttgcaacc atcgacacca cgggcgccgg cgacgctttt gccggtatct
    15241 tcgcctctta catgtccagg ggtatatcta tagccggggc gatagattac gcaagagtcg
    15301 gcgcggccat ttcgactacc aggttcggca cttcgccctc catgccggaa aaagaggaaa
    15361 tagaagcact cctgagtgaa atcaagggag gtgcttccat atgaattacg ttatcgccta
    15421 cgatttaggt actaccggta acaaggcgac gctgtacagt atagacggaa agcttcaggc
    15481 aagcgctttt tcctcttaca ggacttttta tcccggaccc aaccaggttg agcaggatcc
    15541 cgaggagtgg tgggagtcag taaagataac gacatcggag cttttatcga aggccggtat
    15601 cgatccgcga gagatcaagg ctgtcagctt cagcggccag atgatgggag ctattcccat
    15661 cgatcggaac ggaaaaaccc tgagaagg
//
LOCUS       DAOOLY010000022        15680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_3970_length_15680_cov_4.522432, whole genome shotgun sequence.
ACCESSION   DAOOLY010000022 DAOOLY010000000
VERSION     DAOOLY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 15680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15680
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_15680_cov_4.522432"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..1456)
                     /gene="cas1b"
                     /locus_tag="PLD15_02195"
     CDS             complement(473..1456)
                     /gene="cas1b"
                     /locus_tag="PLD15_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HPB62739.1"
                     /translation="MKKNYYLFASGKLGRQDNTLVLEREGKKKFIPVEKIESISSFGE
                     LDLNTRALSFLSQKGIALHVFNHNGWYGGSFVPRRQKVSGKLLTEQVKTLLDPVNRLQ
                     IAREFIDGAMHNMLRILQRQSQKINGYKERIEGLIEIRRRLSDATTINGVMAVEGAFR
                     QNYYPLLAQLTGQEFITRVKQPPLGILNCMISFGNTLLYTRTLNKIYQTQLDPTVSYL
                     HEPCEMRFSLSLDISEVFKPVIIDRLIVSLLNKNMIAENDFDQDLNSTLLTTEGMKKY
                     VQNFDDFMEETVFHPTLKRKVSYNTLLLTECYKLIRSLMADEKYKALHMWW"
     gene            complement(1466..1975)
                     /gene="cas4"
                     /locus_tag="PLD15_02200"
     CDS             complement(1466..1975)
                     /gene="cas4"
                     /locus_tag="PLD15_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPB62740.1"
                     /translation="MELIDRQPRIGGNAVNYLHHCKTQLWFFLHNISMESTSETVQLG
                     KLLHEQYSQGEDKDIIIDEISLDILRPTREIVEIKYSREVKEGHIFQVKYYLYYLKHK
                     LGLTDYTARLTYPRAKKTIVFSLLPDDEELIVKDIAEITSIFNMDNAPAPLFKSGCKG
                     CSYFDLCFS"
     gene            complement(1975..4029)
                     /gene="cas3"
                     /locus_tag="PLD15_02205"
     CDS             complement(1975..4029)
                     /gene="cas3"
                     /locus_tag="PLD15_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPB62741.1"
                     /translation="MSLAKPDVSLVDHTREVLLHAADYLWKVGAKISGSPFSVREIKD
                     AVLIACAFHDLGKGCEGFSFEEKRISHALASAQIALASLKETPLKPYIVQAVLGHHGS
                     RSKDSFTSCKYLKQSTVPNPELAREYGEIRSFVESVYEVALPDIELKVPDPSQLMRYL
                     NNRFFNDAGDREVYAYIQGVLNLADWTASGHKESFDDFPEIDAKNLRPFQKEAASDGD
                     TIIIAPTGRGKTLAALNWAKSTGRRKLSVFLPTVTTVEAMYERYKDELTNDSALVHGN
                     LAYYLYSNEGISDETNEKIFWMKAFENPVTVATLDQLLLMSLNWGRWEPKTVNLAQSA
                     IIFDEIHASQPYTFGILLKAVESLKRYGIPVCVMSATLPSYMIEKLEAVMNDPKVIRD
                     EEGENLKRVTVKLLENVDPSTITLDNYRKGKSVIHCCNTVGAAVKTYQTLSQKVDSKD
                     IVLYHGRFNLEDRGKILSRITSNNRPKIIVATQTIEISLDIDYDVLVTEAAPIDSLAQ
                     RFGRINRQGTNPDGKVFVYPQEENSASIYPPRIVNKALELLENNPVPGGLDLQRMMEK
                     SLDEPETIDSEIEAGKAAWQFLRDINFGIYSMTIEERLADDLVRRMPYKTLQVIPQEY
                     KDSKMNVIEKIGKMVKVPLRDVLGKIEKDEKRLLYAPVNYDSILGYIGPIEDNNIIEE
                     NW"
     gene            complement(4035..4730)
                     /gene="cas5"
                     /locus_tag="PLD15_02210"
     CDS             complement(4035..4730)
                     /gene="cas5"
                     /locus_tag="PLD15_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HPB62742.1"
                     /translation="MKVLRVTLEALSASFRNPNSMNYQDSLPFPPPTTITGVIGAALG
                     KEYMKAQEYCRELFYGVIVEDNCGMAKDQWAIRKIKGGGREIGKAVVTREVIYKPSFK
                     IIVCGPEDKLIEIEKALQNPVYPLSLGRDDEFITSIGYEMVDAQPIKGGIVSDTVLKG
                     DLTGKIHNCDIHSGEIKAFKTYWLSSSFERDSKNPGLRRPSNRAPFTFVNARVDYTGE
                     LLSVNGLNFPIWR"
     gene            complement(4776..5666)
                     /gene="cas7i"
                     /locus_tag="PLD15_02215"
     CDS             complement(4776..5666)
                     /gene="cas7i"
                     /locus_tag="PLD15_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HPB62743.1"
                     /translation="MKKLQVKCLTWGSLSKISMGNPNAGFTEGNIQTAKKIITPDGSA
                     LNYISGQCQRHGIRERFAEVGEALSTPVDGEVETTLGDPASYIDDDLFGYMIAVTGDN
                     RKRTSPVRIAPLVSLFPYRGDRDLLTKTRKASDKGGNMVETEIYSTFMRGGGLIELDR
                     VGKFDSSEIKDKATEIPVVERNRRLSIFLDSLMTLWTGGKQTNILSDISPKFIVFSFQ
                     TVKLPFLLEAVSCDTKGKVDAAKLLDSLSNYSTVTTQVIIGTTGVIDSSEIEAITKDD
                     IQILPIAEAFARVKETLFKL"
     gene            complement(5647..6948)
                     /locus_tag="PLD15_02220"
     CDS             complement(5647..6948)
                     /locus_tag="PLD15_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62744.1"
                     /translation="MKILTRNFWQQAGAIAIRKFAPDALIRGDTFETSSTKKEILSRA
                     LPELLNKNGKPWAPSVRPFLKQYNQIEKLDKFLQDKKPGSKVCSFCNSRVSTLEKLSL
                     LADPLAVKVENFQSFFSHGSGSGEICLDCLFLGIMSGLALFYTSYREGQEYVITYLFP
                     QGKDLEETDKIYRWMKDISEAGGWSNMKVRAKFFPVKPFESLILVLHTLFEKTRFIPR
                     ATYRTMQVSNNGNTNTFLSFNSFESVDELMELLKTGESLGGDFSAFMDSFVVIKQPLV
                     DTSRREEMARMILTFKPIEERLQFMIFELERSIRYLYEMIVATNTVKGVNGLDQETID
                     LCKKAGEIVGNAVFEADSFGDLYSLRNSRSLEDLLLTLSDLEFKYAKEDWKITVPEEF
                     IRILSEDSWKKPKALLVIFAVNKYLARKYASSKNGGEKVAG"
     gene            complement(6965..7138)
                     /locus_tag="PLD15_02225"
     CDS             complement(6965..7138)
                     /locus_tag="PLD15_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62745.1"
                     /translation="MPEFDFDDEELAFDEDYDCDFQGDPCATCWCKGAFCDCGGCFIQ
                     NGRNVCSNCEDND"
     gene            complement(7184..7984)
                     /locus_tag="PLD15_02230"
     CDS             complement(7184..7984)
                     /locus_tag="PLD15_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62746.1"
                     /translation="MKKALFLALVVFMSVSLVAGGLQIGEIGERVEETVDRIMYKDIT
                     DVYNFIDSALDKVIALKGYTDQLNSTEQTSELLEKQVELMDSIYEDFKSLSRMRPKIE
                     KNFRASFKDLERIVDSVESLITAERLEKEKLTGELKELDETKTKVYEIKRDSLTAQIS
                     FIDKRIGILIMLKELIEEPREFTELLAEEVDTFLVTIEETTKVIEQASRTFRTFKEVY
                     CVIETINSVSRIVDLTENMISSWEILLDSVENLNDFITNGLKSQNYTF"
     gene            complement(8010..8270)
                     /locus_tag="PLD15_02235"
     CDS             complement(8010..8270)
                     /locus_tag="PLD15_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62747.1"
                     /translation="MRKVITCILFVLIVFAVLLVTACEKKTDIAPEDQKSWNDVFVRM
                     KEFKEQITRVASPSDYQETQVITVLNLETPFHLLSPDSQERQ"
     gene            complement(8327..8641)
                     /locus_tag="PLD15_02240"
     CDS             complement(8327..8641)
                     /locus_tag="PLD15_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62748.1"
                     /translation="MIVLELSYYFKGKASEVAVNYLKERLWEALDRGKFEIETKLQIG
                     RWTNGGFCLTVLFINCGIDGDPLKEAESLLGFIRQLLERLNIRIDVEKLVKLDGSRVE
                     MN"
     gene            complement(8707..9396)
                     /locus_tag="PLD15_02245"
     CDS             complement(8707..9396)
                     /locus_tag="PLD15_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPB62749.1"
                     /translation="MNIYQKSVFAALEEEGIRTIVNTVNTRGVMGAGLALEFRLRYPE
                     YFDDYQKRCESDLPGAGDAWLYRGSSGTPDIVSLFVKEDWKRPSKPEWIRKAIEKLSK
                     IILSESIERIALPLAGAGKGGVNPKISLSITEEALRDVPSEVYICLDKLPSETEKRML
                     SRLYSLDISALQKLGLTRAISSRVIESLTKVTRFREILSYPGVGIKSYETVFLALLIN
                     SEPSSNQLYLF"
     gene            complement(9410..10000)
                     /locus_tag="PLD15_02250"
     CDS             complement(9410..10000)
                     /locus_tag="PLD15_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HPB62750.1"
                     /translation="MVTVENPLLEHGVKYLYYLAPVKNLRSILRHGIMTKNVVEARKL
                     DYIDCSDREVQALRREKSLHNYVPLFLNTRNAMTYRYQQSGVDFVILRLLSDLVTDYT
                     CKVSDRIAACREAIIMDLTAKALVTFGVREIITVKNFYANHDLKQRLGAEILVHTDII
                     DSKWIESVFLAPGRSRIRDLSIKQLYDNAWNLYFYV"
     gene            10250..12412
                     /locus_tag="PLD15_02255"
     CDS             10250..12412
                     /locus_tag="PLD15_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62751.1"
                     /translation="MDRETVRLTLLAEALELNWKGRVGYSALDELDAFFNREKAVGFA
                     EALKREFLLDQPLEGIMENGFFRGYFAGLMLSSDSLSHEKKEEMARILMERLLVDLKK
                     ADDALPGMDEFFDSAERFEKLFFPFYKDIHRERAKMDFRDNLLMYWCFKNSEYYIPLI
                     LWGNELASFLCEIVDKERFEGEWREKLRVILPPVLASHSIVEFAGYNVDINLPKVESI
                     ASSLFPMLVLMGNEKLTPQQLSEFIRSIAHFTTIDVLLVPAAMAAGGSDEDKAKSVEI
                     INRIYTLLDDSMLGLVLSLSSGMARNGFLYELVMSNDFANLFDRLKILLGSLRKENAS
                     SALAAIASALVEKEESRDSAEEFIEYLMTLESSAGVEVSGILLSKKYGFEHEEFFKKG
                     VNSNIYEEFDYSFFQKIARNSISSLDELYEEIILGYENRYRPEYIIASVEAMLEPSFL
                     SCEDNEVEYSRAPMYTLINSVMEFGDVEEAFNAIVAYCKKYPYNFEFVNALMYIPTLE
                     VDNVSNILLDAGERAFGIISSIVVDEDEDIYLKEGEESPSNKELVTVISNYVDEMVTK
                     KDYRKALKVADTALPLFGIGDSSSLLANSLVAHWRLKDYKKLPELFEEVDDESEPKAL
                     MVMALMNYVNKEIDDAERIILGILEQLPHFIRFLLGNEEPSHEDFDKAEEGDFQHQRK
                     IDALLLAEDFREDWFKLKGAKEWLKKVQRVFDEVSTEW"
     gene            complement(12476..>12667)
                     /locus_tag="PLD15_02260"
     CDS             complement(12476..>12667)
                     /locus_tag="PLD15_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62752.1"
                     /translation="IIIGTDSIYSGNVLGKREIIIGTDSIYSGNVLGKRERRERRETR
                     KTGTDVRSPSVPIFALVFS"
     assembly_gap    12668..12767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12944..14308
                     /locus_tag="PLD15_02265"
     CDS             12944..14308
                     /locus_tag="PLD15_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62753.1"
                     /translation="MDKIKRTQILSVFEGSIYNGFFLITQGFLGTGLALEFGASEPVI
                     ALLGVLPTISQFVQLLAPAMMRLIGNRKRAMMIFATISRLSTAFIPVTLAVGMNRQSI
                     LLMIMAFFSMAASLTGNFWVSLMKDVAPREKAAKFFSSRNIIFTITNMLITLFYSFVL
                     DAVPGTRGFFIISVLGTISALFSLVLLGFHYDPPREITYGRGLFRAVTKDRKFMPYLV
                     FYAFWSFSIAITSPFFSYHELVNMKLGYSFLSILNVFSSLLTMVFYFIWGKIADRIGS
                     QAVLEFGVFGAFIKIILWVFMNEQTVYLLYVDTLIGTISWSAINLCLFTTMLDLLDGV
                     NTESYYALLSFANGGAALVGSLAGGFLASYLNRFGWEILGMRFYGIQILFFVTFVLRG
                     LSLFLLRRVKTRKERSVAGMIFNSAAVIANRLAARPREMVDILVQLNRKRKKGRSDVD
                     EKDD"
     gene            14292..15515
                     /gene="pepT"
                     /locus_tag="PLD15_02270"
     CDS             14292..15515
                     /gene="pepT"
                     /locus_tag="PLD15_02270"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPB62754.1"
                     /translation="MKKMIDRFMRYVSVETTSSHDSDSSPTTESQLKLATILRDELEG
                     LGLEEVSLDEHGYVMATLGANTDERGSVIGFVAHMDTSPDASGKDVKARIVRYEGGKI
                     PLNPDKGIVLDPSTYPELLDYRGKELIVTDGTTLLGADDKAGIAEIVTALEYLKENPR
                     IKHGKIRVCFTPDEEVGKGTDHFDVEKFGADYAYTLDGGALGQLQYETFNAAGAKVKI
                     TGLNIHPGSAKDRMKNSMLIASEFISLLPAAETPAATQGREGFFHLTNMVGTVEETNL
                     NYIIRDHSRERFENRKQLVQGAAAFLNAKYGEGTVDVIVRDSYRNMREMIEPVMFIVD
                     IAARAMRNVGIEPVIEPVRGGTDGARLSFMGLPTPNIFTGGMNYHGRFEYVPVESMVK
                     AVEVIVEICKLFAKR"
BASE COUNT         3592 a         3559 c         4017 g         4412 t
ORIGIN      
        1 acgaagctct gagatcgttt ttttgagaat acttgcgaga gagggtcaaa cattaagtcg
       61 ggtgagtgaa atatgaaaaa aaccaggtta taagcaaagc atgaataagg gttatgttaa
      121 ggtgttaacg gtttttccgt tcttgctaca accgtactta agagcatacc gccaccccgt
      181 tcaagtgggg actagtttca gagcggaaga gcgagaagag tagagaggaa gagagtgtgg
      241 aagagagaga aaagagggcg agactgaaga gatgagatcc cgggagcatt acgagatgac
      301 acgcgaaaaa tgggacagac gctagaagcc cgtcagtccc cattttcgcc tttctctgta
      361 agtctcacaa gcggcagata gtaggtcgta tgagagcact acgtgatgac acgggatgac
      421 aggatgggag atcggcataa cagcgataag acaagttgta tcgattacga gattaccacc
      481 acatgtgaag agctttgtat ttttcatcag ccataaggct tcttatcagc ttatagcatt
      541 ccgtgagaag cagtgtgttg tatgacacct ttcttttgag ggtagggtga aatactgtct
      601 cttccatgaa gtcgtcgaag ttttgtacgt attttttcat cccttcggtt gtgagaagcg
      661 tcgagttcag atcttgatca aaatcgtttt cagcgatcat gtttttattg agaagcgata
      721 cgatcaatct gtcgattata accggcttga atacctcgct tatatcgagt gataatgaga
      781 atcgcatttc gcagggttcg tgaaggtaac ttacggtagg gtccagctgg gtctggtaga
      841 ttttgtttag tgttcttgtg tatagaaggg tgtttccaaa cgagatcata cagttgagga
      901 ttccgagggg aggttgtttg actctggtga tgaattcctg acctgtaagt tgtgcgagca
      961 gtggatagta gttctgtctg aaggctcctt cgactgccat tactccattt atagtcgttg
     1021 catcggagag acgtctgcga atttctatta gcccttctat tctctctttg tatccattga
     1081 ttttttgcga ctgtctttgc agtattcgaa gcatattgtg catggcaccg tctatgaatt
     1141 cacgggctat ttgcaggcga ttaacagggt cgagaagggt tttcacctgt tcggtaagca
     1201 gttttccgga gactttttgt cttctcggga caaagctgcc tccataccag ccattgtgat
     1261 tgaatacatg aagagcgatt cccttttgtg atagaaacga tagagcccgg gtgttgaggt
     1321 ccagttcgcc gaaggaagaa atggattcga ttttctccac gggtatgaac ttcttctttc
     1381 cctctctttc gagaaccagg gtgttatcct gcctgccgag tttgccgctg gcgaagagat
     1441 agtaattttt cttcatgtat gcctcctacg agaaacagag atcgaagtaa gaacacccct
     1501 tgcaaccgct cttgaagagc ggcgcagggg cgttgtccat attgaagata gaggttattt
     1561 cagcgatgtc tttcactata agctcttcgt cgtcaggaag aagggaaaat acaatcgttt
     1621 tctttgccct ggggtatgtg agacgggctg tgtagtctgt gagtccaagt ttgtgcttga
     1681 ggtagtagag gtagtacttg acctgaaaga tgtggccctc ttttacttct ctgctgtatt
     1741 tgatctcgac tatttctctg gtcggcctga ggatgtccag ggagatttcg tcgattatta
     1801 tgtctttatc ttcgccctgg ctgtattgtt cgtgcagaag tttgccgagc tggacagtct
     1861 ccgaagtcga ttccatggag atgttatgaa ggaagaacca gagttgagtc ttgcagtggt
     1921 gtagatagtt gaccgcgttt ccgccgatcc tcggctgtct gtcgataagt tccactacca
     1981 gttttcctcg attatgttgt tgtcttcaat agggccgata taccctagga ttgagtcgta
     2041 attcaccggg gcgtacagga gacgtttttc atctttttcg atttttccga gtacatccct
     2101 caagggaact ttgaccattt ttccgatttt ttcgatcacg ttcattttcg agtctttgta
     2161 ctcttgagga attacctgaa gggttttata gggcatccgg cgtacaagat catctgccag
     2221 tctttcttct atcgtcattg agtagatccc gaagtttatg tctctaagaa actgccaggc
     2281 ggcctttccg gcttctattt cgctgtctat agtttcaggt tcatccagcg atttctccat
     2341 cattctttgg agatccagcc cgcccggcac gggattgttt tctagaagtt caagcgcctt
     2401 gttgacgata cgcgggggat agatagaagc ggagttctct tcctgtggat aaacaaagac
     2461 cttaccgtcg ggattcgttc cctgcctgtt gatcctgccg aaccgctgcg ccagcgaatc
     2521 gatcggggca gcctcggtta ccagtacatc gtagtctata tccaggctga tctcgatagt
     2581 ctgggtggcg actatgatct tcggcctgtt attggaggtt atacgtgaga gtattttgcc
     2641 tctatcttcg agattgaaac gcccgtggta caacacaata tcttttgaat cgaccttttg
     2701 tgagagtgtc tgataagtct ttacggcggc tccgacggta ttgcagcaat ggataacgct
     2761 ttttcctttc cggtaattat ccagtgttat agtagaggga tcaacgtttt ctaggagttt
     2821 cacggtgact cttttgaggt tttcgccctc ttcgtcacgt atgactttgg gatcgttcat
     2881 gaccgcctcg agtttttcta tcatgtaaga gggaagcgtt gcgctcatga cgcaaacagg
     2941 gatcccatat cttttgagcg actctacggc cttcaggagt attccgaaag tataaggttg
     3001 actcgcgtgt atttcgtcga agattatcgc tgactgcgcc agatttacgg tcttgggctc
     3061 ccatcttccc cagttgagcg acatcagtag gagttgatcg agcgtcgcga cggtcaccgg
     3121 gttctcgaag gccttcatcc agaagatttt ttcgttcgtt tcgtcggaga taccttcgtt
     3181 cgaatataag taataggcaa ggtttccgtg tacaagggcg gagtcatttg tcagttcgtc
     3241 tttgtatctt tcgtacatag cctcgacggt ggtcactgtg ggtagaaaga cggagagctt
     3301 tcttctaccc gtcgatttcg cccagttcag cgcggcaagt gttttgcccc tgcctgttgg
     3361 cgcgatgatt attgtgtcgc cgtccgaagc ggcctctttc tgaaagggtc tgaggttttt
     3421 cgcatcgatc tccgggaagt cgtcgaaaga ttctttgtgg ccggaagcgg tccagtcagc
     3481 caggttcagt acgccctgta tgtaagcgta aacctcacgg tcgcccgcat cgttgaagaa
     3541 cctgttgttg agatatctca tcaattgaga gggatcgggg actttcagct cgatatccgg
     3601 caacgcaact tcgtagacgc tttccacaaa cgatctgatc tctccgtatt cccgtgcaag
     3661 ttcggggttt ggcactgttg actgtttcaa gtatttacac gaggtgaagg agtctttcga
     3721 tctggatccg tggtgaccga gtacggcctg aactatgtag ggcttcagcg gtgtttcttt
     3781 cagggatgct agcgctatct gtgcagacgc aagcgcgtgc gaaattcttt tctcttcaaa
     3841 ggaaaagcct tcacaaccct ttcccagatc gtgaaaggcg caggcgatta gcacggcatc
     3901 tttgatctct ctgacggaga acggcgagcc ggagattttc gctccgacct tccacaggta
     3961 gtccgctgca tggagcaata cttctctggt gtgatctacc agacttacat caggttttgc
     4021 aagcgacaat tatttcacct ccagatcggg aagttgagac cgtttactga taggagttcg
     4081 ccggtataat cgacccgggc gtttacaaaa gtgaatggag ccctgtttga cggacgtctg
     4141 aggccggggt tcttcgaatc tctctcgaaa ctggagctaa gccagtaagt tttgaaagcc
     4201 ttgatttctc cggaatggat atcgcagttg tggatttttc cggttagatc tcctttcaaa
     4261 acggtatcgg aaacgattcc gccttttata ggttgagcat ctaccatttc atatcctatg
     4321 gaagttatga attcatcgtc tctcccgagg ctcagtggat atacggggtt ttgaagggct
     4381 ttctcgattt ctatcagttt gtcttcgggt ccacagacga ttattttgaa cgatggtttg
     4441 tagataactt ccctggttac tacggctttg cctatctccc tgccgccgcc tttgatcttt
     4501 ctgatggccc actggtcttt cgccattcca cagttgtctt cgacaatcac gccataaaag
     4561 agttcgcggc agtattcttg agccttcata tattcctttc ccagcgcggc tcctatcact
     4621 cccgtgatgg ttgtgggagg gggaaaggga agcgagtcct ggtagttcat ggagttcggg
     4681 tttctgaagg aagccgagag agcttcgagg gtcactcgca ggactttcac gttttcacct
     4741 ccatagagaa aagcccctcg atgaggggct tctattcaaa gtttaaagag ggtttccttg
     4801 actctggcga aggcttcggc tatgggcagt atctgaatat catctttggt tatagcttcg
     4861 atctcgctgg aatctataac gccggtcgtg ccgattatga cttgcgttgt caccgtcgaa
     4921 taattgctga ggctatccag gagttttgcg gcgtctactt ttcccttcgt atcgcaactg
     4981 acggcctcaa ggaggaaggg gagtttgacc gtctgaaagg agaagacgat gaattttgga
     5041 gagatatccg agaggatgtt ggtctgcttt ccgccggtcc agagagtcat gagagaatcg
     5101 aggaagatgg agagtcttct gttgcgttca acgacgggta tttccgtggc tttgtctttg
     5161 atctctgagg agtcgaattt accgactctg tcgagttcta taagacctcc gcctctcatg
     5221 aaagtggagt atatttcggt ttcaaccata tttccgccct tgtcgctggc ctttctcgtc
     5281 ttggtcagta gatcgcggtc tccgcggtat ggaaagagag acacgagtgg ggcgattctt
     5341 acgggcgatg ttcttttgcg attgtctccg gtaacggcta tcatgtagcc gaagaggtca
     5401 tcgtcgatat agctggccgg atctccaagc gtggtttcga cttctccgtc gacgggtgta
     5461 gagagggctt ctccgacttc tgcgaatctc tctctaatcc cgtgacgctg gcactgaccg
     5521 gagatgtagt tcagcgcgct gccgtcgggg gtgatgattt tcttggccgt ttgaatgttt
     5581 ccttctgtga agcccgcatt ggggttgccc atagagattt tggagagcga accccaggtc
     5641 aggcacttaa cctgcaactt tttcacctcc atttttcgag gatgcatatt ttctagcaag
     5701 gtacttgtta accgcgaaga tcaccagaag agctttaggt tttttccagc tgtcctcgct
     5761 caggattctt atgaactctt cgggaacggt gatcttccag tcttccttcg cgtacttgaa
     5821 ttccagatcc gaaagcgtca gtagaaggtc ttcgagagac cggctgttgc gtagagagta
     5881 gaggtctccg aagctgtcgg cttcgaaaac ggcgttgccg acgatctctc cggccttctt
     5941 gcagagatct atcgtttcct gatccaagcc gttcactcct ttcacggtat ttgtcgcgac
     6001 gatcatttca tacaagtatc taattgatct ctccagttcg aatatcatga actggagcct
     6061 ctcttcgatc ggtttgaagg tcagtatcat tctggccatc tcttcacgcc tggaagtgtc
     6121 tacgagcggt tgttttatca cgacgaagga atccatgaag gccgagaaat cgcctccgag
     6181 agattcgccg gttttcagga gctccatgag ttcatcgaca ctttcgaagg agttgaagct
     6241 cagaaaagtg ttcgtgtttc cgttgtttga gacctgcatt gtcctgtaag tcgcccgggg
     6301 gatgaaccgg gtcttttcga acagcgtgtg gagcacgagg ataagagatt cgaagggttt
     6361 taccggaaag aatttggccc tgactttcat gttgctccag ccgccggctt cggaaatatc
     6421 cttcatccat ctgtagattt tgtcagtctc ttcgagatcc tttccctggg ggaacaggta
     6481 ggttatgacg tactcctgac cttctctgta tgatgtgtag aacagcgcca gtccggacat
     6541 gattcccagg aacaggcagt cgagacagat ctcgccgctt cccgaaccgt ggctgaaaaa
     6601 actctggaaa ttttcaacct tcacggccag tggatcggcg agaagggaga gtttctcgag
     6661 agttgagacg cgtgaattgc agaagctgca gactttgctg ccgggtttct tatcttgcag
     6721 gaatttgtcg agtttttcga tctggttgta ctgtttcaag aacggtctga cgcttggggc
     6781 ccagggcttt ccatttttgt tgaggagttc gggcagagct ctggagagta tttccttctt
     6841 tgtgctggag gtttcgaagg tatcgcccct gatcagagca tcaggggcga acttacgtat
     6901 ggctatggct ccggcctgtt gccagaagtt ccgggtgagg attttcatga tttcacctct
     6961 gtggttaatc gttgtcttcg cagttgctgc atacgtttct cccgttctga atgaagcaac
     7021 cgccgcaatc gcagaaggct cccttgcacc agcaggtagc acacggatcg ccttgaaagt
     7081 cgcaatcgta gtcttcatcg aatgctagtt cttcatcgtc gaaatcgaat tcgggcaaat
     7141 tatcacctcc ttccttctga tttctcttga ctaaagcgag gattcagaat gtgtaattct
     7201 gagattttag accgttggtg atgaagtcgt tgagattctc aacggagtca agaagtattt
     7261 cccaggagct gatcatattt tctgtcagat cgactattct gctcacgctg ttaatggtct
     7321 cgattacgca gtagacttct ttgaatgtcc tgaatgttct gctggcctgc tcgattactt
     7381 tggtggtctc ttcgatcgtg accaggaagg tatcgacttc ctcggccagc agctcggtga
     7441 attctctcgg ctcttcgatc aactctttga gcataatcag tattccgata cgcttgtcta
     7501 tgaatgagat ctgtgcagtc agactgtctc tttttatttc gtagaccttc gtcttcgttt
     7561 catcgagttc cttaagttca ccggtgagct tttccttttc aagtctttca gccgttatta
     7621 ggctttccac agagtcaacg attctttcca gatctttgaa gcttgcccta aagtttttct
     7681 cgattttcgg acgcatgcgg gacaggcttt tgaagtcctc gtagatgctg tccataagct
     7741 cgacctgttt ttccagaagt tccgaagttt gctcagttga gttcagctga tcggtgtaac
     7801 ctttcagcgc gatgacttta tccagtgcgc tgtcgatgaa gttgtacaca tcggttatgt
     7861 ctttgtacat tatccgatca accgtttcct cgactctttc acctatctcg ccgatctgga
     7921 gaccgccagc gacaagagag acagacatga aaactaccag tgcaagaaac agggcctttt
     7981 tcacccgatc acctccatgt gcaagttgtc tattgtcttt cttgagaatc cggggatagt
     8041 agatggaaag gtgtttctag attcaaaact gttataacct gtgtttcctg ataatccgag
     8101 ggtgaagcga ctctggtgat ctgctctttg aactctttca ttctcacaaa cacatcattc
     8161 caggatttct gatcttccgg ggcgatatct gtcttcttct cacaggctgt cacaagaagc
     8221 acagcgaaga caatcaaaac aaacaggatg caggtaatca ctttcctcaa gcctccacct
     8281 cctctgtcac cactgatata gatacacttt cttgaaccgc aacgattcaa ttcatctcaa
     8341 cccgcgatcc atcgagcttc accagctttt cgacgtcgat cctgatattc aatctttcca
     8401 gtagttgcct tatgaaaccg agaagacttt cagcttcttt cagagggtct ccgtcgatcc
     8461 cgcaatttat gaatagcact gtgaggcaaa agcctccatt agtccatctg ccaatctgta
     8521 gttttgtttc aatttcgaat tttcctctgt cgagagcttc ccaaagcctt tctttgaggt
     8581 aatttacggc aacttctgac gccttgcctt tgaaataata tgagagctcc agaacgatca
     8641 ctccttcggg gacctgcgta aggccttcaa gtgatataaa tacactttaa atgtctctag
     8701 agatttctag aagagataaa gctgattgga tgagggttct gaattaatca gcaaggctaa
     8761 aaacactgtc tcgtagctct taattcccac tcctggatat gagaggattt ctctgaatct
     8821 cgttactttt gtcaatgatt cgatgactct gcttgaaatg gcccgggtga gcccaagttt
     8881 ctggagagcg gagatatcca gagaataaag tcttgacagc atacgcttct cagtctcgga
     8941 aggcagtttg tcaaggcaga tatagacttc ggatggaaca tctctcaagg cctcttcggt
     9001 tatggaaagg gagatttttg gattgacgcc accttttcct gctccggcaa gcggcagggc
     9061 gattctttct atgctttccg agaggatgat ttttgaaagc ttttcgattg cctttcttat
     9121 ccactctggc ttcgacggtc ttttccaatc ttccttcaca aacagactca ctatatcagg
     9181 ggtcccactc gagccacggt aaagccaggc atcacctgcc ccgggaagat cgctctcgca
     9241 tcttttctga taatcgtcga agtattccgg gtatcgcagt ctgaattcaa gggccagacc
     9301 agcacccatg acacccctgg tgttcacagt gtttacgatt gtccttatac cttcctcttc
     9361 aagcgctgcg aatacgcttt tctgatagat attcactgct gactcctcgt tacacgtaga
     9421 agtacagatt ccaggcattg tcgtagagtt gtttgatcga tagatctctg atccgggatc
     9481 gccctggagc gagaaaaacg ctttcgatcc acttcgaatc aattatatca gtatgtacaa
     9541 gaatttcggc tcccagtctc tgtttcaggt catgattcgc atagaaattc tttactgtga
     9601 tgatctccct taccccaaag gtcaccagtg ccttcgccgt aagatccatt attattgcct
     9661 ctctacatgc cgctattcta tcggagactt tgcaggtata gtctgtcaca agatcgctca
     9721 gtaatctcaa gatgacgaaa tcgacaccac tctgctggta acgataagtc atagcgttcc
     9781 tcgtattaag gaagagcggg acatagttgt ggagtgattt ttccctgcga agagcctgca
     9841 cttctctatc cgagcagtca atatagtcga gttttcgggc ctctaccacg ttctttgtca
     9901 taataccgtg cctcagaata gacctcaggt tttttaccgg cgcaagataa taaaggtact
     9961 tgactccgtg ctcgagaaga gggttctcaa cagtcaccat attcaccacc tccagagtta
    10021 taaatacact ttccacacct ggagacgata aacggctgac cataagcctt atataagtca
    10081 gagactccat ttttttgcag gaaagcgtgg cccaccccac caacgcactc ataatcttca
    10141 caatctctat aaacccttca ggcaattgaa cttgaacatt ttctttgttt atgtcatttc
    10201 gggcgcgaga tgataatatt gttattattt cggatattcg aggtgaagta tggatagaga
    10261 gactgtaaga ctgacacttc tggccgaagc gctggagttg aactggaaag gccgtgttgg
    10321 atattctgcg cttgatgaac tggacgcttt tttcaataga gagaaagcag ttggatttgc
    10381 cgaagctttg aagcgcgagt ttttgctgga tcagcctttg gaagggataa tggagaatgg
    10441 tttcttcaga ggttattttg cggggctcat gctctcgagt gacagccttt ctcatgagaa
    10501 gaaagaagag atggcgagaa ttcttatgga gaggcttctc gttgatttga agaaagcaga
    10561 tgatgcgttg ccgggtatgg atgagttttt tgactctgcg gagcgtttcg agaagctttt
    10621 ctttccgttt tataaggata ttcatcgcga gcgggcgaag atggatttta gagataactt
    10681 actgatgtac tggtgtttca agaatagcga atattatatc cctctaatcc tctggggcaa
    10741 tgaactggcc tcgtttcttt gtgagatcgt agataaggag aggtttgaag gtgagtggag
    10801 agagaaactg agagtgattt tgcctccggt tctggcttct cattcaatag tcgaatttgc
    10861 tggctataat gtggatataa atcttccgaa ggtcgagagc attgcaagtt cgctattccc
    10921 aatgctggtg cttatgggta acgagaagtt gaccccgcaa cagctatcgg agttcataag
    10981 gagcattgcc cattttacaa cgatcgatgt tttgcttgtt ccggcggcaa tggctgccgg
    11041 aggaagtgac gaagataagg cgaagagtgt tgagataatc aacaggatct acactttgct
    11101 ggacgattcg atgcttggtt tagtattaag tctttccagt ggtatggcta gaaatggctt
    11161 tctgtacgag ctcgtgatgt ctaacgattt cgctaatctc tttgacaggc tgaagattct
    11221 cctgggaagt ctcaggaagg aaaatgcatc gagcgctctt gcagctattg cgagcgctct
    11281 tgttgagaag gaggagtcta gagatagcgc tgaagagttc atagagtatc tcatgacgct
    11341 cgaatcttcc gcgggagtcg aggtttccgg aattctgctc agtaaaaagt acggttttga
    11401 gcatgaagag ttcttcaaga agggtgtgaa cagcaatata tacgaagaat tcgattattc
    11461 gttctttcaa aaaattgcgc gtaattctat tagctctctc gatgagctct acgaggagat
    11521 tatactgggt tatgagaata gatacaggcc ggaatatatt attgccagtg ttgaagcgat
    11581 gctggagcct tcctttcttt catgcgaaga taatgaggtc gaatactcga gagcaccgat
    11641 gtatacgctt ataaactctg tgatggagtt cggcgatgtc gaggaggctt tcaacgctat
    11701 cgtggcttat tgtaagaagt atccctacaa tttcgaattt gtaaatgccc tgatgtatat
    11761 acctacatta gaggtcgata atgtgagcaa cattttgctc gatgccgggg aaagggcttt
    11821 tgggattata tctagcatcg ttgttgacga agacgaggat atatatctca aggagggtga
    11881 agagagcccg tccaataagg agttggtgac tgttatcagc aactatgtgg acgaaatggt
    11941 cacgaaaaag gattatagaa aggcgctgaa ggttgcagat accgctcttc cacttttcgg
    12001 tataggagac tcaagttcgc ttctcgcgaa ttctctggtc gctcactgga ggcttaagga
    12061 ttataagaag ctgcctgaat tgttcgaaga ggtagatgac gaaagcgagc cgaaagccct
    12121 gatggttatg gcgctgatga attatgtcaa taaagagata gacgatgcgg aaagaattat
    12181 tctgggtatc ctggagcagc ttccccattt catccggttt ctgctcggga atgaagagcc
    12241 gagccatgaa gacttcgata aagctgaaga gggcgatttt caacaccaga gaaagatcga
    12301 cgccctgctt ctcgctgaag atttcagaga ggactggttt aagctcaagg gcgccaagga
    12361 atggctgaag aaagtccagc gggtctttga tgaagtcagc acggagtggt gagagccgtt
    12421 gtgggttgtg cgttgttggt aagagccgag aaagagtatc gagggctcgc ttcgctcacg
    12481 agaagacgag agcgaaaata gggactgacg ggctcctgac gtctgtcccc gtttttcgcg
    12541 tctctcttct ctctctcctc tctctctttc caaggacgtt tcctgaataa atggagtctg
    12601 tccctattat tatctctctc tttccaagga cgtttcctga ataaatggag tctgtcccta
    12661 ttattatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct ctctctttcc
    12781 aaggacgttt cctgaataaa tggagtctgt ccctattatt attcctatta ttattatttc
    12841 tctctctccc gctctttcga aggacggctc ggttttactg gactctgcgg gccttttgat
    12901 ctataatgat attacttcat cagcggcgcc ggagatgtct caattggaca aaataaaacg
    12961 cacacagata ctctctgtat ttgaaggctc gatatacaac gggttctttc tgataaccca
    13021 gggttttttg ggcaccggcc tcgcgctgga gttcggcgct tccgagccgg tgatagcgct
    13081 tctgggagtg ctcccgacga tttcacagtt cgtccagctt ctcgccccgg ccatgatgcg
    13141 gctcataggg aacaggaaaa gggcgatgat gattttcgcc accattagca ggctttcgac
    13201 ggccttcatt cccgtgactc tggccgttgg aatgaaccgg cagtcgatcc tgctgatgat
    13261 tatggccttc ttctctatgg cggctagcct tacgggtaat ttctgggtct cgctcatgaa
    13321 ggatgtcgcc ccgagggaga aggccgcgaa gtttttcagc tcgaggaaca tcatcttcac
    13381 gatcacgaac atgctcataa ctctctttta ttccttcgtg ctggacgccg ttcccggaac
    13441 gcgcggcttt ttcattatct ccgtgctggg gacgatcagc gcgctgttta gccttgtact
    13501 gctgggcttc cattacgatc ccccgcgcga gatcacctac ggcagggggc ttttcagggc
    13561 cgtcacgaag gacaggaagt tcatgcctta tctggtcttc tacgccttct ggagcttttc
    13621 gatagcgatt acctcgccgt ttttctcgta tcacgagctg gtcaacatga agctgggata
    13681 ctcgttcttg agcattctca acgttttcag ctcgctccta acgatggtct tctatttcat
    13741 atggggaaag atagccgaca ggatagggag ccaggcggtg ctcgagttcg gtgtgttcgg
    13801 cgctttcata aagattatcc tctgggtctt catgaacgag cagacggtct atcttctcta
    13861 cgtggacact ctgatcggca cgatatcctg gagcgccata aacctatgtc tcttcactac
    13921 gatgctcgat ctcctcgacg gggtgaatac cgagtcgtac tacgccctgc tctccttcgc
    13981 caacggcggg gcggcgctgg taggctctct cgcagggggg tttctggcct cctatttgaa
    14041 caggttcggc tgggagatcc tggggatgag gttttacggc attcagattc tcttcttcgt
    14101 caccttcgtg ctcagggggc tctcgctctt tctgctgagg agagtgaaga ccagaaagga
    14161 gaggtcggtg gccggcatga tattcaattc ggccgcagtc atagccaaca ggctggcggc
    14221 gaggccgcgc gaaatggtgg atatcctggt acagttgaat agaaagagga aaaagggaag
    14281 gagtgatgtg gatgaaaaag atgattgatc gcttcatgag gtatgtctcg gtcgaaacga
    14341 cttcgagcca cgattccgat tcgagcccga ccaccgaaag ccagctgaaa ctcgccacga
    14401 tccttagaga cgagctcgag ggactcggcc tggaagaggt ttcgctagac gagcacggct
    14461 acgtcatggc cacgcttgga gccaacaccg atgaacgggg gtcggtgatc ggctttgtgg
    14521 ctcacatgga taccagcccg gacgcgagcg ggaaggatgt taaggccagg atcgtcaggt
    14581 acgaaggcgg aaagataccg cttaatcccg ataaggggat cgtgctagat ccttcgacct
    14641 acccggagct gctggattac aggggcaagg aactgatagt caccgacggg acgacgcttc
    14701 tgggcgccga cgacaaggcc gggatcgccg agatcgtgac ggcgctcgag tatctaaagg
    14761 agaatccgcg gatcaagcac gggaagatca gggtctgctt cactccggac gaggaggtcg
    14821 gcaaggggac cgatcacttc gatgtggaga aattcggcgc cgattacgct tacacgctcg
    14881 acgggggcgc actgggccag ctccagtacg agaccttcaa cgcggccggc gcgaaggtga
    14941 agataacggg tttgaacata cacccgggct cggccaagga caggatgaag aattcgatgc
    15001 tgatcgcctc ggagttcata agcctgcttc cggcggccga gacgcccgcc gccacacagg
    15061 gcagggaggg cttcttccat ctgaccaaca tggtcggcac ggtagaggag acgaacctga
    15121 actatataat acgcgatcac tcgcgcgaga ggttcgaaaa tcgcaagcag ctggtgcagg
    15181 gtgcggcggc tttcctcaac gccaagtacg gggaaggtac ggtcgacgtg attgtgaggg
    15241 attcgtacag aaacatgcgg gagatgatcg aaccggtcat gttcatcgtg gatatcgcgg
    15301 cccgtgcgat gagaaacgta gggatcgaac cggtgataga gccggtcagg ggaggcaccg
    15361 acggggcgag gctctccttc atgggattgc ccacgcccaa catcttcacc ggcgggatga
    15421 attaccacgg ccgattcgaa tacgtaccgg tcgagtccat ggtgaaggcc gtcgaggtga
    15481 tagtggagat ctgcaagctc tttgcaaaga gataaggagc aagaagagcc gtcctccgtg
    15541 aagagagcat cgggaaacgt ccctggctgt cccggaacac ggaacaacga aggacgtttc
    15601 ccgatatcaa tgactctgga ggtaatatct ctcccgcaac tcgcgtgtta tgaggtcgct
    15661 cgaggtcgcc tttctggagc
//
LOCUS       DAOOLY010000023        15571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4015_length_15571_cov_4.917891, whole genome shotgun sequence.
ACCESSION   DAOOLY010000023 DAOOLY010000000
VERSION     DAOOLY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 15571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15571
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4015_length_15571_cov_4.917891"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLD15_02275"
     CDS             complement(<1..1120)
                     /locus_tag="PLD15_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HPB62755.1"
                     /translation="MDQDWTVVLDFAYLSILLGVASVLKRILTPLSKILIPNSVIAGF
                     LGILLGPEVLKLIPFSYDRLGNLVYHLMAVGFIAIALKKTRRSTAKSSVNTGFLISIS
                     YALQGFVGFIIGIALVGLFFKDLFPPFGLLLALGFAQGPGQAYSLGSQWEALGFTGGG
                     AVGLSVATLGFLWSAFGGIVMLNTMVYRKKQIGIQIERPTVRKRIEATIRDFEFSDID
                     GFTIQILAVGIVYLATYLFLKWFTGLIGGLGTFGETFAQVLWGFHFVIGVLFAMAFRA
                     IYEKFRKSGKYETEYMNDFLLQRIGGGVFDFMVAASIAGISFSVIQHYIWPVIILTTI
                     GGLFIAGYSIWFGKRIYEKAPLEHIVTFFGMHTGTLSTG"
     gene            1228..2559
                     /locus_tag="PLD15_02280"
     CDS             1228..2559
                     /locus_tag="PLD15_02280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB62756.1"
                     /translation="MTFTPLGGGREIGANSYLLSLNGKKILIDAGRHPVKEGYDSLPE
                     LDTIDDIDVIIISHSHYDHISSLPYISTIWPKAPVLMAQENKGLALRILQNSVEVMKK
                     KNEKDGEQPVLYNHGEVNELKRRIYPMEYFQRYTIDDTIEVALYPAGHVMGAASIYLE
                     HNGHRILYSGDFSLSQQLTIPPAQPPLKVETLISEGTYGSKENQLITRFSEIERLSKS
                     VKRVLENKGRVLLPVFALGRGQETIYMLMGLMERGKIPTVPIYTNGMVASISNLYMSN
                     YERISDKDRKWFMQNFQKMVTVAPKNIERLLFSNEPAIFVFSAGMLVEETLSYIFARE
                     MLQSERDGIFFMGYQSPESDGYRVLQAHKGGEKQITLGEETIDMKCKNIDIFNFSGHA
                     DYQELLELPRRLEPEKLIYVHGDEAALENLAEELQYEFQIEIPANLQTVEL"
     gene            2605..2693
                     /locus_tag="PLD15_02285"
     tRNA            2605..2693
                     /locus_tag="PLD15_02285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2639..2641,aa:Leu,seq:taa)
     gene            2743..2816
                     /locus_tag="PLD15_02290"
     tRNA            2743..2816
                     /locus_tag="PLD15_02290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2777..2779,aa:Met,seq:cat)
     assembly_gap    2818..2827
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3205..5925)
                     /locus_tag="PLD15_02295"
     CDS             complement(3205..5925)
                     /locus_tag="PLD15_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPB62757.1"
                     /translation="MENMYQFEKKEAIRRLNSREEGLSSVEVRERLVKFGRNELSEKN
                     KRSPWKILLTQLSSVMIIILIAAAVITAFIGEVRDTIVILAIVALNTILGFSQEYRAE
                     KAMAALKKLSVPKVKVRREGKTVEISAVEVVPGDIVILEAGNIVPADMRLIETSNLKI
                     QESALTGESEAVDKHSEIIEDKNPALGDRKNMAYSSTVVTYGRGTGIVTATGMSTEIG
                     RIATMIQDTREGQTPLQKNLDQLGRVLALLAMGIVAVIFVMGLLRGEELELMFLTAVS
                     FAVAAVPEGLPTVVTIALALGAQRMLKKNALIRKLMAVETLGSVTVICSDKTGTITEN
                     RMRVTLLEMAGQRLDFGKEDEPDPDALPEDDNRALEIMLTGGALCNDATIKPPVHKRQ
                     QVDMIGDPTEGALVYAAYRVGMEKSDLEQAMPRVLEKPFDSERKRMTTIHAIESDPAG
                     AVAADVPEHLREHGIEKYVAFTKGAVDSLMEVCDRVLEGDEIHPLDEEWKSRIVETNN
                     SMASNGMRVLGMAFRAVEESEVGQKTVEERDLIFIGMFGMMDPAREGVKESVVTAKGA
                     GIRTIMITGDHPLTAGYIAASLTMIDDKSQVKTGRDVELLSNDELDRVTASTSVYARV
                     APEHKLKIVGSLQRQGNVVAMTGDGVNDAPALKQADIGVAMGITGTDVTKEVADMVLT
                     DDNFTTIVSAVREGRTIYDNIQKFIRYILASNFGEIWVMLLGPFMGISLPLAPLQILW
                     INLVTDGFPALALAVEPPEDDIMKRPPNPSQQHVFANGGWMVTLIGLILAVFSLGIGY
                     IYWLGDSNGPWRTMIFSTLVFSQLFLVMAIRSRKKSVFRTSLRTNKFFVLAVAGSFLL
                     QLSVIYVPFMQRLFSTQHLGLIDLVISIALGSAILWLYEIKKAFSARRRS"
     gene            complement(5972..7651)
                     /locus_tag="PLD15_02300"
     CDS             complement(5972..7651)
                     /locus_tag="PLD15_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62758.1"
                     /translation="MRLKMVISLTWTLINSHYNIPRSIYHFRRRERLWEPALIAGVIV
                     LMVVMLGPLYGMLMDTMYTQYEAAGIIELFLANPFIMANLFGLVFGVFLMVSIFFFGE
                     DMRVLVSLPLKSTEVLLAKFFVVLLDLMIISLVIVLPPYIYFGIRSGAGFMYWPFMIV
                     AFLLSQVLPLLVSAIIILPLSRVLRFNRHRDSLVYFVSVGVVVAALAFVFVSSRMDPN
                     MSPEDFARMFSDKDALLNKTAGVYPPTILVMRALTRPVVEGFLWLLAFVGLHLAALGG
                     FLWLGNRFYYSIYSSLQENYARRSKLNEGEVAGLMGRQSTPFGALLRREWWYFLKVPA
                     FTFNGFGNVIIFPILLVIAAVAGQTDELGQMIKILENYREIFLPMGILVATLAGGING
                     LATSIFSREGKLIEELKTLPVGVSEIMKAKFIHVETLSMIGPLSAAIALGIIMKISLL
                     EGLLIFAISALAVTFLNMIQMLIDVVRPYLHWDNPQKAMKQNLNVALSIPVVFGFVGG
                     LGYLTYLLRNTLSGWVMALMIAAICVAGTVALWPVLMRRAKALLDRDLSLS"
     gene            complement(7608..8366)
                     /locus_tag="PLD15_02305"
     CDS             complement(7608..8366)
                     /locus_tag="PLD15_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62759.1"
                     /translation="MIEIRQLTKIYSGSVKAVDRVNMTVKRGEIFGFLGPNGAGKTTT
                     IKMIVGLLKPTEGQIMIEDIDVISSPVEAKLKMGYVADEPLVMEKITGVQYLNLICDV
                     FKIPPSVRIERAERLLQNFKLSDAVKDPVSTYSHGMRQKLSLIAALIHNPDLWILDEP
                     IVGLDPESAFILKQMMRNHVKAGNTVFFSTHVMEVAERICDRIGIINKGKLEFVGTVK
                     ELRDLRGQGSLEQLFLEVTGSETENGDFSYLDAN"
     gene            complement(8383..9249)
                     /locus_tag="PLD15_02310"
     CDS             complement(8383..9249)
                     /locus_tag="PLD15_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62760.1"
                     /translation="METPELTLKISNLKKTYPGEVQAVRGVSLEMESQHIYALLGPNG
                     AGKTTVLKSVLGFIDYSGSVEIFGNGVDQIRDRISFVPEEKSFYENLNVEKALRLASK
                     ISGSLDGKHARELINHYKLPFNKKIGTFSNGMKTALYISLALSTDSDLYILDEPTWGL
                     DPILREDTLEFIREKVIVGKTVLYTSHIIPEVERIADEFFIMVNGRIRFAGTMDGIKE
                     KYRIIHMPLRTKVNIESKDSFAVAKELNRLSVIVENDEKIEEFYRIDDATISVPDLEE
                     FFQILVRSDNGK"
     gene            complement(9231..9605)
                     /locus_tag="PLD15_02315"
     CDS             complement(9231..9605)
                     /locus_tag="PLD15_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB62761.1"
                     /translation="MWLDIDMRSPSPIYDQIKRGVREEIIKGKLKEGDTLPSIREMAS
                     QIRVNPNTVARAYRELEMEGTIMARQGLGYIVVSDGEKVRDSVFDNLSEELKEPILRL
                     KKSGISLDKLLEVIENIWKHQS"
     gene            complement(9689..11002)
                     /locus_tag="PLD15_02320"
     CDS             complement(9689..11002)
                     /locus_tag="PLD15_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HPB62762.1"
                     /translation="MQRKLVLLLVILFSVLAVASVDIASQYINGLYEGRFEEIYQMQD
                     ETMKKAQPVTVLKNLKTQLDVQLGKPIATLDIQQVQQGEYMVYVLATEFKVGIFDFIV
                     TLNTEGKVAGFFVRPSSYKKAETQPVEVPEERPLPDYANPANYTEQNITVGKEPYYLP
                     ARLMVPNGLESYPLVIILQGSGTHDIDGTLGPNKPYRDLAAGLASRGIATLRYPKKYY
                     VYPQQAAEIGINADAEYIEDALTIIAQMKGIPQFSSIYLAGHSMGGMVAPWIAQEAGV
                     DGMILLAGSPVKFAQISLDQNLDLVGDQLSEEQKAQVKDFFTKMLNQEIPPETDLGQG
                     ITAGYYYSFDGYFCMPVLEETEMPVLVVNAELDFQTPKKYFDIFVEKLGDRPNITLKL
                     LPGLNHIFNETDGTIKSVEEYDRPGYVAVELIDLLESWIKEAEQR"
     gene            complement(11118..11888)
                     /locus_tag="PLD15_02325"
     CDS             complement(11118..11888)
                     /locus_tag="PLD15_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB62763.1"
                     /translation="MLYKELNEMKLRVILIIAVLVISLVATIVMRPYASQMMSEMAGQ
                     LEELPDFLKKLIGDTSSLTKLDDDNYYLLSQWQGKNFGQFLPLVVLLIAFPIFAKEFD
                     KKTIYFLLSRKNRKDVFMAKYLIGLGVILLVTAVLSLLGPIVMNLAGYGVNFSGTLKA
                     LLQQLVGAAFFYSLFTLISVMSRDQVKPVVMGIIIVIGLPIIGIIEAISWLNPYPFIL
                     ATTVIQNGTIDWIYLVALLAVTIVLTAVDYMVFKKKEL"
     gene            complement(11878..12732)
                     /locus_tag="PLD15_02330"
     CDS             complement(11878..12732)
                     /locus_tag="PLD15_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62764.1"
                     /translation="MSARTIEIGNLRKSYGNFEAVKGIDLKIVAGKVNALLGPNGAGK
                     TTTLKSILGLVEYSGEIKILGKTVEEVRNSISFVPEDKSFYENLTPGKAIKMCSMVLD
                     NFLPSKAEELMRHFDLPMGKKISSFSHGMRTAAYLSIAMAQSADIFIFDEPTWGLDPL
                     RRDEVLEMISGLSESGKTVLYTSHIIPEIEKIADVFSIMYKGEILYSGELKALDERFA
                     LFCLPAGTELERNEYLAVVKETGAIKVLTDDKNTMEKLGKIAGAKEEKVDLEVFFSLL
                     VRGNKNAL"
     gene            12907..13983
                     /locus_tag="PLD15_02335"
     CDS             12907..13983
                     /locus_tag="PLD15_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HPB62765.1"
                     /translation="MKRLLVFLLLSLAATVSFGCTGFRLIAKDGTVITARTLEFDFDL
                     MSEITVVPRGTYMTALTTGWQGLSWNVKYGYVGIDALGMDLLEDGLNEAGLSAGTFYF
                     DQYAGYQELDKAQSRKTLSQYDLVGWILGNFATVEEVKEAIKQVRVYGFTVAHSEILK
                     MPLHYAVYDTKGDCVIIEYIDGELHLHDNPIGVIANSPPFDWHIQNLSNYVNLSSANI
                     KSSKLGQFEIKAISGGTGLLGLPGDNTSPSRFVRAAFYSNGSVRPSNSEEAVTLGWHI
                     INALDIPEGLVINEVLGFVVTRHIAYWSSVKDLTNRIYYYRTYKDMNIRMVDLKRIDL
                     DSGNIRFIPMSYKQQYIDETDLIK"
     gene            14358..>15571
                     /locus_tag="PLD15_02340"
     CDS             14358..>15571
                     /locus_tag="PLD15_02340"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HPB62766.1"
                     /translation="MKKIVLLLCVLITITGFMLGNVETELSAYLDHETIDNIMRSLWL
                     YRDGNYLFEGLSEEVLTNLLDVKNHNESYTQRMQHRLAWMLDNPDYFTPDYTQKYTTF
                     MKYATSLSPHKAYVLTQFLGLDSNKGYEEVPRVGYLYIPKDHAPQLGFQLGWHFFVGN
                     CVDEEGNEYGIQLMFWRYSLLPPEMARNFGLTDIENQIMDMHFAISRAGDRHYRSKPI
                     VVGGTTGLLEFKSNPFTFAIGNNSITSLQEDDFTPLRLEAKGWDKSQEHEVEIEIGIT
                     IEQMKDVVLNGNQGAVPSVGGVGTLYYSITNFRMIPEDSYLVIDGEKVALVEGKFWFD
                     HQWANGLSPAGNPRDLLVRMAKTQAAPEPSGWDWFMVQFDNETEIGMSAMHTLENTPF
                     YFQTGINPPGVMS"
BASE COUNT         3664 a         4130 c         3846 g         3921 t
ORIGIN      
        1 tcccggtcga tagagttccg gtgtgcattc cgaaaaaggt cactatgtgt tccaggggag
       61 ccttctcgta tatcctcttg ccgaaccaga tggagtagcc ggcgatgaag agaccaccga
      121 tggtggtgag aattattacc ggccagatgt agtgttgaat gaccgagaag gagattccgg
      181 cgatcgaggc cgcaaccatg aaatcgaaga caccgccgcc gattctctga agcagaaaat
      241 cgttcatata ctcggtttca tacttcccgg acttccggaa tttttcgtat atcgccctga
      301 aggccattgc aaatagaacc ccgatgacga aatggaagcc ccacaacacc tgggcgaaag
      361 tctccccgaa agtacccagt cctcctatca ggccggtgaa ccacttcagg aaaaggtacg
      421 tggccagata gacgatgcca accgccagaa tctggatcgt gaagccgtcg atatcggaga
      481 actcgaagtc ccttatcgtc gcttcgatcc ttttcctcac ggtaggtctt tcgatctgga
      541 tccctatctg tttctttctg taaaccatcg tgttgagcat cacgataccg ccgaaggccg
      601 accagagaaa accgagggtg gccacgctca acccgaccgc accgccgccg gtgaatccga
      661 gagcctccca ctgactgccg agagaataag cctgtccggg accctgagcg aaaccgagag
      721 ccagcaacag accaaaaggc gggaagagat ccttgaagaa gagccccacc agcgctatgc
      781 cgattatgaa tccaacgaac ccctgaaggg cataggatat gcttatcaaa aagccggtgt
      841 tgacggacga ctttgccgtc gaacgcctgg tcttcttcag cgcgatggcg atgaaaccga
      901 cggccatcag gtgataaacg agatttccaa gacggtcgta agaaaaaggt atcagtttca
      961 gaacctccgg tcccaggaga attcccagaa agcccgctat aaccgaattc ggaataagaa
     1021 tcttgctcag aggcgtgaga atccttttga ggacagaagc aactcccagc aggatagaca
     1081 ggtaagcaaa atccagtacg acggtccaat cctgatccat ataatcacct tcttttttcg
     1141 gaacaaagct attttatcaa attgtcttca agttaattcc cctcttcttt gttagaattg
     1201 acttgacaaa aaaggaggaa atttgcaatg acatttacac cgctgggtgg cggcagggag
     1261 ataggggcaa actcttacct cttgagcctt aacgggaaga agatacttat cgacgcgggg
     1321 aggcaccctg tgaaagaagg ctacgactcg ctgcccgaac tcgacacaat agacgatatc
     1381 gatgttataa tcatctctca ttcccactac gatcatatat cttcattgcc gtatatctct
     1441 accatatggc ccaaagctcc cgtccttatg gcccaggaga acaaggggct ggccctgagg
     1501 attcttcaga actccgtaga agtgatgaaa aagaaaaacg agaaagacgg cgagcagccc
     1561 gttctctaca accacgggga ggtcaacgaa ctcaaacgcc gcatctaccc catggagtat
     1621 ttccagagat acacgattga cgacaccata gaagtagcgc tctacccggc cggtcacgtc
     1681 atgggagccg cttcgatata cctcgaacac aacgggcaca ggatactgta ctcgggcgac
     1741 ttttccctct cacagcagct gactataccg ccggcccagc cgccgcttaa ggtcgaaact
     1801 ctaatatcgg aaggtaccta tggctcgaag gaaaaccagc taatcacacg tttcagcgaa
     1861 atcgagagac tgagcaagtc agttaagagg gtactcgaaa acaaaggtcg ggttcttctt
     1921 cccgtcttcg cgctgggcag gggccaggaa acgatctaca tgctcatggg gctcatggaa
     1981 agaggaaaga tcccgaccgt gccgatctac accaacggaa tggtggcctc gatcagcaac
     2041 ctgtacatga gcaactacga aagaatcagc gacaaagaca ggaaatggtt catgcaaaac
     2101 ttccagaaaa tggtcaccgt ggcgccgaag aacatagaaa gactcctctt ctcgaacgaa
     2161 ccggcgatct tcgtcttctc ggccgggatg ttagtggaag agaccctgtc ctacatattc
     2221 gccagagaaa tgctccagag cgagcgcgac ggtatcttct tcatgggtta ccagagccct
     2281 gaatccgacg gctacagagt gctgcaggct cacaagggcg gcgagaaaca gataaccctt
     2341 ggagaggaaa ccatagatat gaaatgcaaa aacatagata tattcaactt ctccggtcat
     2401 gccgattacc aggagcttct agaattaccg agaagactcg aacctgagaa attaatctac
     2461 gtacacggtg atgaggccgc gctcgaaaac ctcgcggaag aactccagta tgaattccag
     2521 atagagatac cggcaaacct tcaaactgtg gagctctaga ttgcttgaca aggtctaggg
     2581 gtgatgttat catagtttac ggttgccggg gtggcgaaac tggcagacgc aacggactta
     2641 aaatccgttg gggcgagggc tccgtgtggg ttcgattccc atccccggca ccatgcaagc
     2701 agaaaagcag gtcattcata tacaagagat tttgacatac ggtgcggggt ggagcagtct
     2761 ggcagctcgt tgggctcata acccaaaggt cacaggttca aatcctgtcc ccgctacnnn
     2821 nnnnnnnagc atgcggttca tacccgcaga gtcgtcagtt cgattctgac cgccgccacc
     2881 agtaatgtta gtacaattgt tactgaacaa aacgaaaaaa gaccccggaa taaccggggc
     2941 tttttctttg ttagttatat ctagatccag ttcaggaaca ccctgtacgg gatctcgttc
     3001 cgttctttct gaggaggaaa gaccgggatc cttactagga acttgtcaga tgacgcgaag
     3061 gggttgtcat cgcgcgaatg ctcatgcgct gtcatcccgt gaagcctgcc ctgaaatgat
     3121 cctattcagg attactaaac gggatctcat ctcttcagtc tctccctctc tcttcctctc
     3181 gctcttctca ttttacggtc agcgtcagga acgtcttctc gcagagaagg ctttctttat
     3241 ttcgtataac cagagtatgg cgcttcccag cgcgatcgag atgaccagat cgatcaatcc
     3301 caggtgctgt gtcgagaaga gtctctgcat aaatggaacg tagatgacgc tcagttgcag
     3361 caggaaactt ccggccacgg ccagcacgaa gaatttgttg gtgcgcaggc tagtcctgaa
     3421 caccgatttc tttctcgatc ttatcgccat caccaggaag agctgcgaga agaccagcgt
     3481 cgagaagatc atcgttctcc acggaccgtt gctatcgcca agccagtata tgtagcctat
     3541 acccagcgag aagaccgcca gaatgagacc tatcagcgtc accatccagc ccccgttggc
     3601 gaagacgtgc tgttgcgaag ggttgggagg ccgcttcatt atgtcgtctt cgggcggttc
     3661 gacagcgaga gccagcgctg gaaacccgtc ggtcacaagg tttatccaca gtatctggag
     3721 tggtgcgagc ggcagagata tgcccatgaa cggaccgagg agcattaccc agatctcgcc
     3781 gaagttcgag gccagtatgt atcttatgaa cttctgtatg ttgtcgtaga tagtccttcc
     3841 ctcgcgtaca gccgagacga tagtggtgaa gttgtcgtcg gtcagcacca tgtcggctac
     3901 ttctttggtc acatccgttc cggttatgcc catcgccacg cctatgtcgg cctgtttcaa
     3961 ggccggcgcg tcgttcacgc cgtcgccggt catggccact acgttgccct gcctctggag
     4021 cgagccgact atcttcaatt tgtgttccgg ggctaccctc gcgtacactg aagttgaggc
     4081 cgtgaccctg tcgagttcgt cgttggacag cagctctaca tcccggccgg ttttgacctg
     4141 ggatttgtcg tctatcatcg tcagactggc ggcgatatat ccggccgtca ggggatggtc
     4201 gccggtgatc attatagtgc gtatcccggc ccctttggcg gttacgaccg actctttgac
     4261 accctccctt gccgggtcca tcataccgaa catccctatg aagatcagat ctctctcctc
     4321 cacggttttc tgcccgactt cgctctcttc gaccgctctg aaggccatgc cgagcacgcg
     4381 catgccgttg gaggccatcg agttgttggt ctccacgatt ctactcttcc actcttcgtc
     4441 tagcgggtgt atctcgtcgc cttccagaac tctgtcgcac acttccatga gcgaatcgac
     4501 ggcccccttg gtgaaggcga cgtatttctc tattccatgt tctcgtaagt gttcgggaac
     4561 atctgcagcg acagcaccgg ccggatctga ttcgatggcg tgtatcgtag tcatcctctt
     4621 tctttcggag tcgaaaggct tttcgagaac tctgggcatc gcctgttcga gatcgctctt
     4681 ttccattccg accctgtagg ccgcatagac cagcgcgcct tccgtcgggt cgcctatcat
     4741 atccacctgt tgtcttttgt gaaccggtgg ctttatggtg gcgtcgttgc agagcgcccc
     4801 tccggttagc attatctcca gcgctctgtt atcatcctcc gggagcgcat cgggatctgg
     4861 ttcgtcttcc tttccaaaat cgagtctttg cccggccatc tccaggaggg ttacgcgcat
     4921 cctgttctcg gttatcgttc cggttttatc cgagcatatc accgttactg agccgagggt
     4981 ttcgacggcc atgagcttgc gtattagagc atttttcttg agcatccgct gggcgcccag
     5041 ggcaagcgct atcgtaacta ctgtcggaag tccttccgga acggcggcta cggcgaacga
     5101 tacggctgtc aggaacataa gctcgagctc ttctcccctg agaagcccca tcacgaagat
     5161 gacggcgacg atgcccatcg ccagaagcgc cagaaccctg ccgagctggt cgaggttttt
     5221 ctggagcggc gtctggcctt cgcgggtatc ctgtatcata gtggcgattc gaccgatctc
     5281 cgtgctcata ccggtcgccg ttacgattcc cgtacccctg ccgtaagtga caacggtgct
     5341 cgagtaggcc atattcttcc tgtcgcccag cgccgggttc ttatcctcta ttatctcgct
     5401 gtgtttatct accgcttccg attcgcccgt cagggccgat tcctgtattt tgaggttact
     5461 ggtttcgatg aggcgcatat cggcgggaac tatgttgcca gcctccagga tcactatatc
     5521 acccgggacg acttcgaccg cgcttatctc gaccgttttg ccctcgcgtc tcaccttcac
     5581 tttcggaacg gaaagctttt tcagggcggc catggccttt tcagccctgt actcctggga
     5641 gaagccgagt atagtgttga gcgcaactat cgccagtata actatcgtgt cccggacctc
     5701 gccgatgaag gccgttataa cggccgcggc gatcagtatt atgatcatca ccgatgataa
     5761 ctgggtgaga agtatcttcc acgggcttct cttgtttttc tcgctcagtt cgttcctgcc
     5821 gaattttacg agtctctccc tgacctctac agaagagagc ccctcttctc tggagttcag
     5881 cctccgtatc gcttcttttt tctcgaactg gtacatattt tccatatata tggcctcccg
     5941 cgcacattcc ctttggtaca agtttatcac ttcatgacag cgagagatct ctgtccagaa
     6001 gggcctttgc ccttctcatg agcactggcc ataacgctac cgttccggcg acgcatattg
     6061 cggctatcat gagagccatc acccagccgg acagggtgtt tctcaacaga taggtcaggt
     6121 aacccaggcc gccgacgaaa ccgaagacca ccggtatcga gagggcgacg ttcaggttct
     6181 gcttcatggc cttctggggg ttgtcccagt gcaggtatgg cctcaccaca tcgatcagca
     6241 tctgtatcat gttcaggaaa gttaccgcca gagcgcttat ggcgaagatc agaagtccct
     6301 ccagcaatga gattttcatt attattccca gtgcgatcgc ggccgaaagc ggtcctatca
     6361 tactgagtgt ttcgacgtgg atgaacttcg ctttcattat ctcagaaaca ccaaccggca
     6421 gggttttcaa ctcttcgatc aacttgcctt cgcgactgaa tatggacgtt gccagtccgt
     6481 ttattccacc ggccagcgtt gctaccagga tacccatcgg aaggaatatc tccctgtaat
     6541 tctcgagaat ttttatcatc tgtcccagct cgtcggtctg accggccacg gccgctatca
     6601 ccaggagtat cggaaagatg atcacgttgc cgaagccgtt gaaggtaaag gcgggaacct
     6661 tcagaaagta ccaccattca cgtctcagca gcgcaccgaa gggcgtcgac tgtcttccca
     6721 tgagtcccgc tacctcgcct tcgttgagct tgctcctcct ggcgtagttt tcctggagac
     6781 tcgagtatat ggagtagtag aacctgttcc ccagccacag gaaaccgccc agcgccgcca
     6841 ggtggagccc tacgaaagcc agaagccaca gaaaaccttc cacaacgggt ctcgtgagcg
     6901 ccctcataac gagtatggtt ggcggatata cgccggccgt cttgttgagt aaggcgtctt
     6961 tgtcggagaa catgcgggcg aaatcttccg ggctcatgtt tgggtccatc cgactgctca
     7021 cgaagacgaa ggccagcgcc gcgacgacga cgcccacact cacgaaatag actagcgaat
     7081 ccctgtgcct gttgaatctg agaacacgcg acagcggcag tattattatc gcgctcacca
     7141 gtagcggcaa cacctgactg agcagaaagg cgactatcat gaagggccag tacatgaatc
     7201 ccgcgccgct cctgataccg aagtagatat acggcgggag cacgatcacc agggaaatta
     7261 tcatcaagtc cagcagaacg acgaagaatt tggccagcaa gacctcggtc gatttcagcg
     7321 gcaacgacac gaggacgcgc atatcctccc cgaagaagaa gatgctgacc atgaggaaga
     7381 ctccaaatac gagaccgaaa aggttggcca tgatgaaggg attggccaga aagagctcta
     7441 taattccggc cgcttcgtac tgggtgtaca tagtgtccat aagcatgccg tagagaggac
     7501 ccagcatgac gaccatgagc actatcactc cggctatgag cgcaggctcc cagaggcgtt
     7561 ctcttcgcct gaaatggtag atagatctcg ggatgttgta atgggagtta attagcgtcc
     7621 aggtaagaga aatcaccatt ttcagtctca ctaccggtca cctccaggaa gagttgttca
     7681 aggcttccct gccctctcag atctctcagt tccttcacag ttcccacgaa ttcaagtttt
     7741 cccttgttta ttatgcctat ccggtcgcag atcctttcgg ccacttccat cacatgggtc
     7801 gagaagaaca cggtgtttcc cgccttcacg tggttcctca tcatctgctt aagtatgaag
     7861 gccgattccg ggtctaggcc gacgatcggc tcgtcgagta tccaaagatc ggggttgtga
     7921 atgagcgcgg cgatgagtga aagcttctgg cgcataccgt gagagtaagt cgaaaccggg
     7981 tccttgacgg cgtcggagag cttgaagttc tgcaagagtc tttcggccct ttcgatgcgt
     8041 acggacggcg gtattttaaa gacatcgcag atgagattta aatactgcac ccctgttatt
     8101 ttctccatca ccagaggttc gtcggccaca taacccatct tcaacttcgc ttctaccggc
     8161 gaagagatca cgtcgatgtc ttctatcatg atctgtcctt ccgtgggttt gagaagcccc
     8221 acaatcatct ttattgtggt ggtctttccg gcgccgtttg gaccgagaaa gccgaagatc
     8281 tccccgcgct tgacagtcat gttcactctg tcaacggcct ttacgcttcc gctgtagatt
     8341 ttcgtaagtt gcctgatctc gatcatcttt ttcctccttg atttatttcc cgttgtcgct
     8401 ccttacgaga atctggaaaa actcttccag atccggaacg gaaatggttg catcgtcgat
     8461 cctgtagaac tcctctatct tctcgtcgtt ttccacaata accgaaagtc tgttgagctc
     8521 tttagcgaca gcgaaggaat ctttgctttc tatattgact ttcgtcctca gaggcatgtg
     8581 gattatcctg tacttctctt tgataccgtc catagtgccg gcgaagcgaa tccttccatt
     8641 gaccattatg aagaactcgt cggcgatccg ttcgacctcg ggtattatat gggaggtgta
     8701 gagaacggtc ttgccgacga tcaccttctc ccttatgaac tccagcgtat cttccctcaa
     8761 aatcggatcg agtccccagg tcggttcgtc caggatgtag agatcgctgt cggtcgagag
     8821 cgccagactt atgtataggg ccgttttcat gccgttggag aaggttccga tcttcttgtt
     8881 gaacggcagt ttgtagtgat tgatcagctc cctcgcatgt ttcccgtcga ggctgcccga
     8941 aattttcgag gccagcctca gggccttctc cacgttgaga ttttcataga agctcttctc
     9001 ctcggggacg aaagagatcc tatctctgat ctggtcaacg ccgtttccga aaatctccac
     9061 acttcctgaa taatcgatga agccgaggac cgatttcagc acggtcgtct tgccggcccc
     9121 gtttggaccg agaagcgcgt aaatgtgctg gctttccatc tcgagagaca cacccctgac
     9181 ggcctggacc tctcctggat aggtcttttt aaggttactt atcttgagag tcagctctgg
     9241 tgtttccata tattttcaat cacctccagg agtttatcca gagagatgcc cgacttcttc
     9301 agacgaagta ttggctcttt gagctcctcg gaaagattat cgaacacgga atctctcact
     9361 ttttctccat cactgactac tatatacccc agaccctgtc ttgccataat ggtcccctcc
     9421 atttccagtt ctctgtaagc cctggcgacc gtgttggggt tgacccttat ctgggaggcc
     9481 atctctctta tcgaaggaag agtgtcaccc tctttcaatt tccctttgat gatctcttct
     9541 ctgacacccc tcttgatctg gtcgtaaatt ggcgagggag atctcatgtc gatatctagc
     9601 cacacattat caccttcaat cgtgagttac gtcttgagag ccatcctgcg cccttctaaa
     9661 aaggcgagaa gacggtgaaa gcctcttctc acctctgttc ggcttctttt atccaactct
     9721 ccagaagatc tatcagctcc acggctacgt atcccggtct gtcgtattct tctacactct
     9781 ttatcgtgcc gtcggtttcg ttgaatatgt ggttcaggcc cggcaggagc ttcaaagtta
     9841 tgttcggcct gtcgcctagc ttctcgacaa agatgtcgaa atacttcttc ggagtctgga
     9901 aatctagctc ggcgttgact accagcaccg gcatctcagt ctcttcaagc accggcatgc
     9961 agaagtaccc gtcgaagctg tagtagtagc cggccgttat accctgtccc aggtctgtct
    10021 cgggtggtat ctcctgattc agcatcttgg tgaaaaaatc ttttacctgt gccttttgct
    10081 cttcggacag ctgatcgcca accaggtcga ggttctgatc gagactgatc tgggcgaatt
    10141 tcaccggtga accggcaagc aatatcattc cgtcaacgcc ggcctcttgc gcgatccagg
    10201 gcgccaccat tccgcccatc gagtgtccgg ccagataaat ggagctgaac tgggggattc
    10261 ccttcatctg agcgattatc gtaagtgcat cttctatata ctccgcatcg gcgtttattc
    10321 cgatttcggc cgcctgctgg ggatacacat agtacttctt ggggtatctc agggtggcta
    10381 tgcccctcga ggctagaccg gccgctagat ccctgtaggg cttgttgggg ccgagcgtgc
    10441 cgtcgatgtc gtgcgttccc gagccctgga ggattatcac gaggggataa gattccaggc
    10501 cgttgggaac catgagcctg gcgggcagat aatacggttc cttgcccacg gttatattct
    10561 gttcggtgta gttcgccgga tttgcataat cgggcagtgg cctctcttca gggacttcga
    10621 cgggctgcgt ttcggccttt ttgtagctcg aaggtcttac gaagaaaccg gccacttttc
    10681 cttcggtgtt cagcgtcact atgaaatcga agataccgac cttgaattcg gtggcgagaa
    10741 cataaaccat atactcgccc tgctgaacct gttgaatatc cagcgtggct atcggtttgc
    10801 cgagctggac atcgagctgg gtcttcaggt ttttcagcac cgtgacgggc tgggccttct
    10861 tcatcgtctc gtcctgcatc tggtagatct cttcgaacct gccttcgtaa aggccgttga
    10921 tatactggct tgcgatatct accgaagcca cggccagtac ggaaaagaga atcacaagca
    10981 ataaaaccag tttcctctgc atacttcaaa acctcccgta tgaaatcgat cgaaaagttg
    11041 atcgtctatc tttctatatt tatcccccgt cgccgcatta agtatcgcaa gagaggggtc
    11101 tatccggtcc agtatcatca caattctttc tttttaaaga ccatgtaatc tacggccgtc
    11161 agtacgatcg tcactgccag aagcgccacc aggtagatcc agtctatcgt tccgttctga
    11221 atgaccgtcg ttgccagaat gaacgggtag gggttcagcc atgaaatcgc ttcgattatt
    11281 ccgattatcg gcagtcctat cactattatg atacccatta ccacgggttt cacctggtcc
    11341 ctgctcatca ccgatatcag cgtgaagagg gagtaaaaga aggcggcacc cacaagttgc
    11401 tggagaagag ccttcaacgt tcccgagaag ttcactccgt aaccggccag gttcatcacg
    11461 atcggtccga gcagggacaa aacggcagta accaataaga tcacaccaag tccgatcaga
    11521 tacttcgcca taaagacgtc tttcctgttt tttctcgaga gaaggaagta gatagtcttt
    11581 ttgtcgaact ctttggcgaa gattggaaag gctatcaaca atacaaccag gggtaagaac
    11641 tgtccgaagt tcttgccctg ccactgactg agcagatagt agttatcgtc gtcgagtttc
    11701 gttaggctcg aagtgtcgcc gatgagtttc ttcagaaagt cgggcaattc ctcgagctgt
    11761 ccggccatct cagacatcat ctgggaggcg tacggtctca tcacgatcgt tgccacaagg
    11821 gaaattacca gcactgcgat gataagaatc accctgagtt tcatttcgtt cagttcctta
    11881 tagagcattt ttattccccc ttacgagaag gctgaagaag acttcgagat ctaccttttc
    11941 ttcttttgcg cctgctattt ttccgagctt ttccattgtg ttcttatcgt cggtcaaaac
    12001 tttgatcgcg cccgtttcct tgaccacggc caggtattca tttctttcca gctcagtccc
    12061 ggccggcagg cagaagaggg cgaacctttc gtccagagct ttcaactctc cgctgtacag
    12121 tatctcgcct ttgtacatta ttgagaagac gtcggctatc ttttcgatct ccggtattat
    12181 gtgggaagtg taaagaacgg tctttccgct ctcggacaaa ccgcttatca tctccagcac
    12241 ttcgtccctt ctgagggggt ccagtcccca ggtcggttcg tcgaatatga agatatccgc
    12301 cgattgggcc atcgcgatcg aaaggtacgc cgccgtcctc attccgtgag agaaggagga
    12361 gatcttttta cccatcggga gatcgaaatg tctcatgagt tcttcggcct tcgagggaag
    12421 aaaattgtcc agaaccatcg agcacatttt gatggccttc ccgggagtga ggttttcgta
    12481 gaagctctta tcctccggta cgaaggatat actgtttctc acttcttcca cggtctttcc
    12541 cagaattttt atctcaccgc tatactcgac cagacccagg atcgatttca gtgtggtagt
    12601 ctttcccgcg ccgttcggcc ccaggagggc gttgaccttt ccggcaacta tcttcaggtc
    12661 gatacctttg accgcttcaa aatttccata cgactttctc aggttgccta tttctatcgt
    12721 tcttgcagac accggaatca ctcccttttc atctatcaca tctgtactat atcaaatagt
    12781 acagttagtt gtcaaggggt ggattttttt cgcgttttca atctatccag aagcggtaaa
    12841 catgtataat ccgattgtga agctcttcaa acggtgcttc ctcaaaaagt gaaaaggggg
    12901 attcttatga aaagacttct ggtatttctt cttttatctc ttgcggcaac cgtctctttc
    12961 ggttgcaccg gcttcagatt gatcgccaaa gacggaaccg tgatcacggc caggactctc
    13021 gagttcgatt tcgacctgat gagcgagata acggttgttc cgcgcggtac ctacatgacc
    13081 gccctgacca caggctggca agggctcagc tggaacgtga agtacggtta tgtcggtatc
    13141 gatgccctgg ggatggatct gttggaggac ggactcaacg aggccgggct gtcggccggg
    13201 actttctatt tcgatcagta tgccggttat caggaattgg acaaagcgca gagcaggaag
    13261 accctgtcgc agtacgatct ggtgggctgg atactgggca atttcgccac cgtcgaagag
    13321 gtgaaggaag cgataaagca ggtccgcgtc tatggcttca cggtggccca cagcgaaatc
    13381 ctgaagatgc ccctccacta cgcagtttac gacacgaaag gcgactgtgt gatcatagag
    13441 tacatcgacg gtgagctcca tctccacgac aaccctatcg gcgttatagc caacagcccg
    13501 cctttcgact ggcatatcca gaacctctcc aactacgtca atctgagttc cgcgaatatc
    13561 aagagctcga agctgggaca gttcgagata aaggcaataa gcggcggcac cggcttgctg
    13621 ggattgcctg gcgacaacac ctctccgagc aggttcgtga gggccgcctt ctattctaac
    13681 ggcagcgtta gaccttccaa cagcgaagag gcggttacac taggatggca catcatcaac
    13741 gcgctggaca tcccggaagg actggtgatt aacgaagtgc tcggctttgt cgtcaccaga
    13801 catatcgctt actggtcgag cgtgaaagat ctcaccaaca ggatctacta ctacagaaca
    13861 tacaaagaca tgaacataag aatggtggat ctcaagagga tcgatctcga ttcgggcaac
    13921 atcagattca tcccgatgag ctacaaacag cagtatatag atgaaaccga cctgatcaaa
    13981 taactagaaa tgcaaacaat tattgaggcc ggaggagagg aagagagaga agtagagaga
    14041 gcgtgacacc ttctcgtgag cgaagcgaac ctctcgaatg cgctctttat cgtgttttta
    14101 cccacaaccg ttcttagaaa gcaagctgac cgccgaaggt tactggtttt gtgatgactg
    14161 aagaattcgc tatccgtaaa tactgtcatt tcaaacatct tttgtgatag tatctattta
    14221 ggctttgttt cagatttttt tatttaactg ttatcattgt tgaatccttt ggaggccttt
    14281 tttttcgaga caccctgtct agatttttat tgtttcaaaa aacgaatgtg gcttgtaaaa
    14341 gccgagagga gagcctcatg aaaaagattg tgttgttgct ttgtgttctg ataaccatta
    14401 caggattcat gcttggaaac gtagaaacgg agctctcagc gtacctggat cacgagacta
    14461 tcgacaatat tatgaggtcg ttatggcttt accgggatgg caactatctg tttgaagggc
    14521 tttccgaaga ggttctcacc aacttgcttg atgtgaaaaa tcacaacgaa agttatacac
    14581 agcgcatgca gcaccggctt gcctggatgt tggataatcc agattatttt acacctgatt
    14641 atacccagaa atacactact ttcatgaagt acgccacttc tctctcgccg cacaaggctt
    14701 atgttttgac acagttcctt ggtctggaca gcaataaggg gtatgaagag gttccacggg
    14761 tcgggtacct ctatatcccc aaagatcacg ctcctcagct gggcttccag ctgggctggc
    14821 atttctttgt cggaaactgt gttgatgaag agggtaatga gtacggtatc cagcttatgt
    14881 tctggcgata ttcgcttctt ccgcccgaaa tggccagaaa tttcggtctt acagatatcg
    14941 agaatcagat aatggacatg catttcgcga tcagccgcgc cggagataga cattaccgga
    15001 gcaaaccgat cgtggtcggt ggcacaacag gccttttgga gttcaaatcc aacccattca
    15061 cttttgcgat agggaacaac tccatcactt ccctgcagga agatgatttt actcctctca
    15121 gactggaggc aaaaggctgg gataaaagcc aggagcacga agtcgagatt gagataggca
    15181 taacgataga acagatgaaa gatgtggtgc tcaacggaaa ccagggggcc gttcccagtg
    15241 ttggtggcgt tggcacgttg tactattcaa tcacgaattt tagaatgatt cccgaagaca
    15301 gttatctggt aatcgacggg gagaaagtcg ctctggtgga ggggaaattc tggttcgacc
    15361 atcagtgggc gaacgggctt agccctgcgg gaaatccccg ggatctgctt gtaaggatgg
    15421 ccaaaaccca ggcggctccc gaaccctccg gttgggactg gttcatggta cagtttgaca
    15481 atgaaacgga aatcggaatg tctgccatgc atactcttga aaacacacct ttctatttcc
    15541 agacggggat caaccccccg ggagtgatgt c
//
LOCUS       DAOOLY010000024        14894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4281_length_14894_cov_4.413775, whole genome shotgun sequence.
ACCESSION   DAOOLY010000024 DAOOLY010000000
VERSION     DAOOLY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4281_length_14894_cov_4.413775"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1601
                     /locus_tag="PLD15_02345"
     CDS             213..1601
                     /locus_tag="PLD15_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPB62767.1"
                     /translation="MKAKLILLLVLIATMALGTTLQKSDEYLQKQNEIKLAIEFSLTF
                     EEAKEIIVKHYPELKSDEDVRRFIVDRDIQRRTVDGFEMYFGEFEHNLFTRDPELVKR
                     EPEWSATNALLVKYLLSEYGPRQTPFANFKPDFSPYFNPKDYLVEYTVHTERNLLPEK
                     GSLKIWFPLPLQTATQENIYILEVTPEEAVVGVPRIDGDIAYVLLEFELSDLEEDLDI
                     SVKFTFRHYQQQFDIDPEKVGNYDTESSLYRDYTKSEGSIYFDERFEQLARSIVGEET
                     NPYLQAKKIYYYVVENILYSYMAHVTIGAEGIPESVYSFEHGYGDCGTQSIIFSALCR
                     SIGIPARAPGGFQIFTGQLGSHFWAEFYLPNYGWVPVDTSAGQIATYTVGITEEERQD
                     FIDYFFGNQDPLRFVVQNSVDFMPDERPTDNQFLEITMQNPYVESEFGNEEFGVAMTI
                     LDSFEMKTILLR"
     gene            1784..2479
                     /locus_tag="PLD15_02350"
     CDS             1784..2479
                     /locus_tag="PLD15_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB62768.1"
                     /translation="MKVLLFIFLCVIVVTVIVFGINNSPKKDVQEILVRDYQGIRLDD
                     LSAFRENSIKGVQYVNEENYILRIGGLVERPYEMSYSTLQELEHMEKVVTLFCVEGWT
                     AKMLWEGIPLVELIEKAGPTAEVDNVIFKSTDGYTTSLSLDYVKERNIILADKLNGIK
                     LPPAQGFPFIVVAEDKWGYKWARWVEEIELSDDETYRGYWEILGYSSDGSLDKPMFER
                     YDKPESSGGKELR"
     gene            complement(2503..3573)
                     /locus_tag="PLD15_02355"
     CDS             complement(2503..3573)
                     /locus_tag="PLD15_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB62769.1"
                     /translation="MKTIDRVETRKGRIYDFLPGIYRLTNNERLVEHFKELTPGYRPV
                     GEIAEWVRITLKKLPESILEKMDLYFNWQTPLAMRLSPIISENGLKTVDEFLKALRAV
                     PPEDLLRDFLLIGLGPRAVGFDESIISQMLGDQQKALVFLAKKAVLTPHQKAVMLDFF
                     SNPVSMKDDFVYLLEWYAEHIFPTISYDEGAMEESERDLIKNLQSRGEEYLLKLIDNM
                     RYDELEDGRKILLAISYFVENAQGGVFHPRARNDLFVVGYRFVNSVLEDDPLRQAAAR
                     YRAMGEPRRIKLLRMLFGKRYTGYDISRKLKMSNAEVTETFSNLVACNLIKTYRTQEG
                     VYFTADRDEVIDLLIGQLSEND"
     gene            complement(3675..4511)
                     /locus_tag="PLD15_02360"
     CDS             complement(3675..4511)
                     /locus_tag="PLD15_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB62770.1"
                     /translation="MSIGIVTDNTCNLKSAEIEKLGVYIVSLYVNRSGKYTRALDLDL
                     DSYYNELGTVAELPTTSQPSPQDFMEAFDRALQNFDVLVVPLISGKLSGTFVSASIAS
                     RDFDMPVHVIDTLLTENGLGMLIRNLSKMIEEGSDTDTLVKYATDFYKKIRTIFSVDD
                     LNYLYKGGRIGKAKALMGNLLKMKPVLQMDEGELKPVENVRGNKKLLQSMVDHTLKGK
                     DPAELKRVDIVYIKRSEDATELKRILLERVPTVEVEISLIEPVIGTHLGPEGIGIISE
                     WA"
     gene            5142..6437
                     /locus_tag="PLD15_02365"
     CDS             5142..6437
                     /locus_tag="PLD15_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62771.1"
                     /translation="MKKILVVVLVLLSVVAFSKTTITFWHAMSGSNLTAVDNVVKGFN
                     ESQNEIEVVAQFTGSYAETLTKAIAAYRTGTHPHIVQVYEVGLQTMLDSGAILPVYQL
                     AEPGFDWGDVVTPILNYYSVNDQLYSMPFNSSTAILYYNKDIFAAAGLDPNKPPSTFD
                     ELYEMGKKIVQSGASQGGISFGWPAWVFEQMHATHAQFYANNENGREAKASQVLFNGE
                     FGQKVFIEWMKWAQDKVFVYGGREYSANTAFLTGQVAMLIQSTSSVGSIQKTASFNVG
                     TSFLPRMEGYPRGNSVIGGATIWVLKGKSEAENKAVWEFLKYIIRPEVSLQWHKSTGY
                     FPSSNTTVKLLMDEGWFASQPNHLTAFLQILSGSNIPEAQGVRLGNFVAIRDVVDSAV
                     EKAVQYKGKDYEKEAKRILDDAAKECNNILREYIQLYGD"
     gene            6505..7386
                     /locus_tag="PLD15_02370"
     CDS             6505..7386
                     /locus_tag="PLD15_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62772.1"
                     /translation="MQRRFPNRVLPYILLSPSIVIVIIFLIVPTVQSIYMSFFRVSPF
                     GDRKFFVGFENFTRLFQSSAYLHSLIITLIFAVLVVLIGLSLGTVIAVLLNQKLRGLF
                     VYRTFFIWTYAISPAIAGTIWALMFNPSSGPIVYIIRSITGIGINWMTDRNLALAVVV
                     IAASWKMLGYNIIFYLAGLQAIPEELLESASIDGASGVTKFFKIILPLLSPTTFFLLI
                     MNMLYAFFDVFGLIDIMTKGGPGDATELLVYKLYRDGFISMNTGFASAQSVVLFVFVA
                     ILTVLQFKYTERKVFYG"
     gene            7406..8236
                     /locus_tag="PLD15_02375"
     CDS             7406..8236
                     /locus_tag="PLD15_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB62773.1"
                     /translation="MKKKNLKKMVIYLVLTGMALVVMFPIYYAFSMSTLTAADSYSYP
                     PRFTTGKNIIENYVTAWKTVNMGRLMLNSGFTAFIVATGKIGFAILAAFAFTYFGDFK
                     GKFFFFGMILITHMLPLPVRILPTYELMKSFDWINTYYALTIPFFASATGTLLFRQLF
                     MTVPTSLSDAARIDGAGPMRFLFNVLLPLSKTNMGALFLIEFNFIWNEYLWPLIITTT
                     NDMRVVQIGIKMLLASEAQAAEWNIIMAGTITAMIVPLIMLLIFQKTFMSGFSLKEEK
                     "
     gene            8414..9193
                     /locus_tag="PLD15_02380"
     CDS             8414..9193
                     /locus_tag="PLD15_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB62774.1"
                     /translation="MRGLRESWEYYNDIATRYDYMYEEPYWQLYHRLVERLVDDHIAR
                     RSRVLDLGTGTGRWALRLAEDGHDVIAVDPAGEMLKVAKMKAELAELKIEFHEVGGED
                     LPFEKASFNCVLAMGDVLSYAMEPEKVLKKIGEVLRKGGKLLASVDNAWAFLQDFLSL
                     ADYSMAQKLLDERKIPIGDRSVSNKSFLSRAYFPGEISGILRSNGFELVDMASLIAFY
                     PYNEVSLASNISRACEWEYRYCRQKETFSRSEHLFFCAIKR"
     gene            9198..9836
                     /locus_tag="PLD15_02385"
     CDS             9198..9836
                     /locus_tag="PLD15_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62775.1"
                     /translation="MAKRRKQSPAMIIGLTLILASIVGFVFSLMAVNRVGQVRKEMTE
                     LKNSYERKIEKMEADFQTKLASIERLLGGGGTLEKYIVAKNFLSNTSVDLDAILTEMQ
                     HQSDRPYFRVFVTGTKEVWIGMKKSSSDSAYYFQKNFKPGLSQEKFYYLKNPEIETKY
                     TIMLNKDAYIRTGVPESVYLIFFGFDSGKLSRMPGVEIANLKDAFNLYIPGG"
     gene            10055..10816
                     /locus_tag="PLD15_02390"
     CDS             10055..10816
                     /locus_tag="PLD15_02390"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB62776.1"
                     /translation="MKRFEGKVAMVTGGGSGIGADAAEAFAHEGATVVVTDIDEEKGR
                     GLVEKINGHGGRAIFLKHDVSSAEETEKVVKTIVEKFGRLDIAFNNAGISGPSVSVAE
                     YPVDTWERIISINLSGVFYSMKYEVEQMLKQSGGAIINNSSILGKVGFSNASAYTASK
                     HGVVGLTKAAALEYASRNIRINAINPAFIKTPLLEAAGMGQTSPAYEMMVALHPVGRF
                     GEPREVTSVVLFLASDEASFIHGESLMVDGGYTAR"
     gene            10897..11688
                     /locus_tag="PLD15_02395"
     CDS             10897..11688
                     /locus_tag="PLD15_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPB62777.1"
                     /translation="MSELFNREDFLKNLIKRIHDDPENLEKVKEDFMRVVREMTPIEI
                     AKIEQELVNEGMPAESIQLMCNIHLDVFKEALSEDEIDVEPWHPLHILVEEHRDILNR
                     SKELRDRAGRIRNGDLTQSDVQAIANLLDYMDEVEKYFLKEENVLFPYLEKHGLVQPP
                     AIMWKEHDEVRGLRKKLREMIDDGTVEPAKVFDLSIGIGEIFSNHIFKEHKILFPSAL
                     KLLSLEEWRDIRIQFDGIGYFAYRPMPVEFSLSNRKWKPPVGKAF"
     gene            11658..12086
                     /locus_tag="PLD15_02400"
     CDS             11658..12086
                     /locus_tag="PLD15_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB62778.1"
                     /translation="MEASSREGLLNLGSGYLTFDQLRAMMSSLPFDITFADGDDTVRF
                     FSEGPDRIFVRTRAVIGRKVQNCHPQKSVNVVNKILADFKAGQRDVADFWLNLGPKMV
                     YIKYIALRDSQKRYIGTLEVTQDIAPLRELEGEKRIYDAL"
     gene            12181..13305
                     /locus_tag="PLD15_02405"
     CDS             12181..13305
                     /locus_tag="PLD15_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB62779.1"
                     /translation="MVRREMGKTGEKVSALGFGCMRLPTLDSGIDIPEATRMLRHAID
                     NGVDYVDTAWGYHNGQSEPFVGEALKDGYREKVNLATKLPSWLIKSHEDMDHYLNEQL
                     KRLQTDVIDFYLLHALNGRFWDNLAKNNVFDFMDRAKRSGRIKHIGFSFHDTLETFKK
                     IVDAYDWEFCQIQYNFLDTDYQAGTDGLNYAAEREMGVIVMEPLRGGKLVRRIPADVR
                     KIWDLAPQKRSPVEWALRWVWNDPAVGVVLSGMSSMEQVEQNLEIAGAALPNSLTSQE
                     LSLIARVREIYISRIKINCTACGYCMPCPNNVAIPDSFEVYNDAAMFDDVEGARRSYS
                     NFFKPENRASMCVECGRCEELCPQKIPIIEKLKEVAALLE"
     gene            13444..14787
                     /gene="glmU"
                     /locus_tag="PLD15_02410"
     CDS             13444..14787
                     /gene="glmU"
                     /locus_tag="PLD15_02410"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPB62780.1"
                     /translation="MTGIVLAAGLGKRMRSNLPKVAHMIIDRPMVNWVVSSLKEAGVD
                     RVVVVTGYRAEIIESLLESDILTVRQNEQLGTGHAVMMAKDFLDDDDVVIVAGDEPLI
                     KPSTLREMVRRRREADLDVLFLTMVPPDPSGYGRVVKRDGKVEIVEERDASEEIRNIK
                     EVNTGIYAFRGAFISNSIVTLKNSNDQGEYYLTDTVGIAGRADTLVVADAEEVLGINN
                     RAQLAQVARIARKRINNTLMEAGVTIVDPETTYIGPGVKIGIDSVVEPMTFIYGKTTI
                     GQNCLIGPMTRIQDSKIADNVEIVRSEVLEADVREGARVGPYSRLRPGAVIMEQAHVG
                     NYVELKKSILGKRSKANHLTYLGDTIIGEDANIGAGTITCNYDGKNKFKTSIGDRAFI
                     GSNSSLVAPVEIGEGSVTAAGSVITDDVPPYSLALGRARQINKEGKYKKNREEKD"
BASE COUNT         4020 a         3493 c         3791 g         3590 t
ORIGIN      
        1 tctcgacctc cagaaagata ttatcacggt cgatggtgga aatatatctg gcttaaagct
       61 ctcaatcgac gatggtgatg ccggactgaa aattaatgca aaaacctgga ctttgggtcg
      121 aaagatcaca tgtattgttt gaattatagg tatgaatagt tatatactac ctgtgttatg
      181 tcttcttgaa agacaagatg gggggtacag ttatgaaagc gaaactgatt cttttactcg
      241 tactaatagc tacgatggct ctcggaacca cgcttcagaa atctgacgaa taccttcaaa
      301 agcagaatga gattaaactc gcgatcgaat tctcgctgac tttcgaagag gccaaagaga
      361 tcatagtcaa gcactatccc gagctcaaga gtgatgaaga cgtaagaaga ttcatagtgg
      421 atagagatat tcagaggcgt accgttgacg gttttgagat gtattttgga gaatttgaac
      481 acaatctctt cacccgcgac cccgaactgg tcaaaagaga acccgaatgg tcggccacca
      541 acgccctgct ggtcaaatat cttctcagcg aatacgggcc gcggcagaca cctttcgcca
      601 atttcaaacc tgactttagc ccctatttca atcccaaaga ctaccttgtt gaatacacag
      661 tacacaccga aagaaatttg ttaccggaaa agggctctct gaagatctgg tttcccctac
      721 ctttgcagac ggccacacag gagaacatct atatcctgga agtgactcca gaggaagcag
      781 tcgtcggggt tcccagaatt gacggtgata ttgcatatgt tcttcttgaa ttcgaactct
      841 ctgatctaga agaagacctg gacatctcgg tgaagttcac cttcagacac taccagcagc
      901 agttcgacat agatccggaa aaagtgggaa actacgatac tgaaagctca ctgtataggg
      961 attacacgaa gtcggaaggc agtatctact tcgatgaaag gttcgagcag cttgccagaa
     1021 gcatcgttgg ggaagagaca aacccgtatc tccaggcgaa gaaaatatac tattatgtcg
     1081 tagaaaacat cctgtatagt tatatggctc acgttacgat cggggccgaa ggaatacccg
     1141 aaagtgttta ctcattcgaa cacggctacg gagactgtgg aactcagtcc attatcttct
     1201 cggcactctg cagatccatc gggataccgg cgagggcacc gggaggcttt cagatattca
     1261 ccggccagct cgggtcacat ttctgggccg agttctatct gccaaactac ggctgggtcc
     1321 cggtcgatac ctctgcgggc cagattgcga cctacaccgt gggcatcacc gaagaggagc
     1381 gacaggattt catcgattac tttttcggca accaggaccc tttaaggttt gttgtccaga
     1441 attctgtgga tttcatgcca gatgagagac cgaccgataa tcagttcctg gagatcacca
     1501 tgcaaaatcc atatgtggag agcgagttcg gaaacgagga gttcggagtc gccatgacga
     1561 tcctcgattc ctttgagatg aagacgattt tgctcagatg aagtttagat ctgaactctt
     1621 taaaggttgg agaagtcata atttcgcttg acaaggcttc ttctcctacc accctgcccg
     1681 gagagtcttc tccgggcttt tctttgccag gctggcgaac tacaaattaa gcatcgatgt
     1741 ctaattataa cggtaccaga taagtcgtcg gaggtgttaa ctggtgaagg ttctgctttt
     1801 catattctta tgcgtaatcg tagttacagt gatagtcttt ggaataaaca actcaccgaa
     1861 aaaagatgta caggagatac tggtgagaga ttaccaggga atcaggctag atgatctcag
     1921 cgctttcaga gagaactcca taaagggtgt acagtacgtc aacgaagaga attacattct
     1981 caggatcggc ggacttgtcg aaaggcctta cgagatgagt tactccactc ttcaggaact
     2041 ggaacacatg gagaaagtcg tgactctttt ctgcgtcgag ggatggaccg ccaaaatgct
     2101 ttgggaaggg ataccgctcg tggaactcat agagaaggcc ggcccaacag cggaggtgga
     2161 taacgtgatc ttcaaatcca cagacggcta cactaccagc ctgagtttgg attatgttaa
     2221 ggaaaggaac atcatactcg ccgataaatt gaatggtata aaactacccc cggcgcaggg
     2281 ctttcctttc atagtggtgg cggaagataa atggggctac aagtgggctc gctgggtaga
     2341 agaaatagaa ctttcggacg atgagactta ccgaggatac tgggagatcc ttggatatag
     2401 cagcgacggt agcctggaca agccgatgtt cgaaaggtac gacaaaccgg agagttccgg
     2461 cggaaaggag ctgcgctaac cgttccagac gctttggtcg ttctagtcgt tttctgacag
     2521 ctgtccgatc agcaaatcga tcacttcatc gcggtcggcg gtgaagtaaa ctccttcttg
     2581 agtgcggtag gtctttatga ggttgcaggc gaccagattg ctgaaggtct cagtaacttc
     2641 tgcgttggac atcttcaact ttctggagat gtcgtagccg gtgtagcgtt tcccgaagag
     2701 catgcgcaaa agctttatgc gtctgggttc tcccatagcc ctgtacctcg cggcggcctg
     2761 acgaagtggg tcgtcttcga gaactgaatt cacgaatctg tatcctacga cgaagaggtc
     2821 gtttctcgct ctgggatgga agacaccgcc ctgggcgttc tccacgaaat aggagatggc
     2881 cagcagtatc ttcctcccgt cttccaactc atcgtaacgc atgttgtcta tgagttttag
     2941 aaggtactcc tcgcccctgg actggaggtt tttaatcaga tctctttcgc tctcttccat
     3001 ggcaccttcg tcgtaggaaa ttgtgggaaa gatgtgctcg gcgtaccatt ccagaagata
     3061 cacgaaatcg tctttcatcg aaactgggtt ggagaagaaa tccagcatga cagctttctg
     3121 atggggtgtc agcacggctt tcttggccag gaaaactagc gccttttgtt ggtcgccaag
     3181 catctgcgag attatgcttt catcgaagcc tacggccctc ggaccgagac cgatcaggag
     3241 gaagtccctc agcagatctt ccgggggtac tgcccgcagg gctttcaaga actcgtcaac
     3301 cgttttgaga ccattttcgc ttatgatcgg actcaatctc atggcaagcg gcgtctgcca
     3361 gttgaagtac agatccatct tttccagtat cgactcaggt agctttttga gagtgatcct
     3421 tacccattcg gctatctcgc cgacgggcct gtagcccggc gtaagctctt tgaagtgttc
     3481 gaccagcctt tcgttgtttg taagcctgta tatccccggt aaaaaatcgt agatccttcc
     3541 ctttcttgtt tcgactctat cgatcgtttt cacctgaaca cccccagtct ttgatagtta
     3601 accatataaa aaacggcaat tcaagcgctt caaaagcccc tccgctcgaa tcgccgtcaa
     3661 atttcgggat cagactatgc ccattcgctg attataccga tcccctccgg gccgagatgc
     3721 gtacctataa caggttcgat caacgagatc tcgacctcga cagttggaac cctttcaagc
     3781 agtattctct tcagctctgt ggcatcttcg cttctcttta tgtacacgat atcgaccctt
     3841 ttgagctcgg ccgggtcctt acctttgaga gtgtgatcca ccatactctg gaggagcttt
     3901 ttgtttcccc tgacgttttc aacgggcttc agttcgcctt cgtccatctg gagaacgggt
     3961 ttcatcttca ggaggtttcc cataagggct ttagcctttc cgatcctacc ccccttgtag
     4021 agataattga ggtcatccac ggagaagatc gtgcggattt ttttgtaaaa atcggttgcg
     4081 tacttgacca gagtatctgt atcactgcct tcctcgatca ttttcgacag gttcctgata
     4141 agcatcccca gaccattttc cgtgagcagc gtgtctatga cgtgaacggg catatcgaaa
     4201 tccctggaag ctatgctcgc cgaaacgaaa gtgccgctca gcttacctga aatcagagga
     4261 acaacgagaa catcaaagtt ttgtagtgcc ctgtcgaagg cctccataaa atcctgggga
     4321 gagggctgcg aagtggtcgg aagttccgct acagtcccca gctcgttgta ataggaatcg
     4381 agatcgagat cgagcgctct ggtgtattta ccggacctgt tgacgtagag cgatactata
     4441 tataccccca gcttctcaat ctcagcggac ttaaggttgc acgtattgtc tgtgactatg
     4501 cctatgctca taaacaatcc ctccgttcag actagattgc tcaaattata gaccatagaa
     4561 gtcggaatat cgataaaaga cgatcggttt tttcctgaag gtatggttac cctctgcgca
     4621 actgtaacga gtttcccggt aacacattcg ctctttcttg ctttttgaga attcctggag
     4681 atattatttc aaatactgaa ttcactaatc acagtattta attcaataaa tctaatatat
     4741 gagccaaaat aatcaattat cttcttttca tcgattaaat atctgcgtaa cacaaattat
     4801 tgtgccaagc tatagtataa atgtactaag atataaaatt atttgagaat tgagtataaa
     4861 taatccaaag tttcaggagg gatcgattat gaggaaattt ttcgggttct gctttctcgt
     4921 gattctggta gttgacagag tcttcgtgga aatgatcgaa ggaataggtg acaggagagt
     4981 caggagaagc gtaaaataaa agaccgtata gactgtattc acgtattcaa aaggtttttt
     5041 tacccttcgg cgcgagtcga agggtttttt gtcgagagtg gtggtgttat aatcattttg
     5101 cgatccatcg tacttccatt tttcaacaag gaggcatttg catgaagaag atactagtcg
     5161 ttgttctggt tcttctctcg gtcgtggctt tttccaaaac aacaatcact ttctggcacg
     5221 cgatgagtgg cagcaacctc accgccgttg ataatgtcgt caagggattc aacgaaagcc
     5281 agaacgagat cgaagtggtt gcgcaattca ccgggagtta cgccgaaact ctcaccaaag
     5341 cgatagcagc ttacagaact ggaacacacc cccacatcgt acaggtttac gaagtcggac
     5401 tacagaccat gctagatagc ggagctatcc ttccagttta ccagctggcc gagccgggtt
     5461 tcgattgggg agacgtcgtc accccaatcc tgaactacta ctcggtgaac gatcaactct
     5521 actccatgcc attcaactcg tcgactgcca tcctctacta caataaagat attttcgcgg
     5581 cggccgggct ggaccccaac aagccaccgt caaccttcga cgaactgtac gaaatgggca
     5641 aaaagattgt acaatcgggc gcatctcagg gaggtatctc gttcggctgg ccggcatggg
     5701 tcttcgagca gatgcacgca actcatgcgc agttctacgc caacaacgaa aacggtagag
     5761 aggccaaggc ttcgcaggtt ctattcaacg gagagttcgg tcagaaggtt ttcatcgagt
     5821 ggatgaagtg ggcacaggac aaggtcttcg tctatggtgg tagggagtac agcgccaaca
     5881 ctgctttcct caccgggcaa gtcgccatgt tgatccaatc gacttcttcg gtgggttcca
     5941 ttcagaagac cgcctctttc aacgtcggga cttccttcct gccgagaatg gaaggttacc
     6001 ccagaggaaa ctcggttatc ggaggcgcca cgatatgggt tctcaaggga aagtccgaag
     6061 ctgaaaacaa ggctgtctgg gaattcctga agtacataat aaggcccgaa gtctctctgc
     6121 aatggcataa gagcaccggt tacttcccct cgagcaacac gaccgtcaag ctactcatgg
     6181 atgagggttg gttcgcttct cagccaaacc acctgaccgc cttcctgcag atactcagcg
     6241 gatcaaacat acccgaagca cagggagtca gactgggtaa cttcgtcgcg ataagagatg
     6301 tagtcgattc agccgtcgaa aaggccgttc agtacaaggg aaaggattac gaaaaggaag
     6361 ccaagaggat tctcgacgat gcggcaaaag agtgcaacaa catactgaga gagtacattc
     6421 aactatacgg tgattgattt gcttttcgac ggggaggggt ttttcccctc cccttaatcc
     6481 attttcaggc ggtgaaagaa aaaaatgcag agaagatttc ccaatcgagt gctcccgtac
     6541 atcttgctct ctccctcgat tgttatagtt attatctttc tcatcgtacc gaccgttcag
     6601 tccatatata tgagcttctt cagggtttct ccattcggcg atagaaagtt cttcgtcggt
     6661 ttcgagaact tcaccagact cttccaatcg tcagcctatc tgcacagcct gataataacc
     6721 cttatcttcg ccgtattagt ggtgctcatc ggcctgtcgc tgggaacggt catagccgtg
     6781 ttactgaatc agaagctgcg cggtctattc gtttacagga cttttttcat ctggacctac
     6841 gccatctcac cggccatcgc cgggaccata tgggcgctca tgttcaaccc gtcgagcggt
     6901 ccgatagttt atataataag aagtatcacg gggataggga tcaactggat gaccgataga
     6961 aacctcgctc tcgcggtcgt tgtcatagcc gcttcgtgga agatgctggg ttacaacatt
     7021 atcttctacc tggccggcct gcaggctatt cccgaagaac tcctggaatc tgcctctata
     7081 gacggtgcct ccggcgtaac gaagttcttc aagatcatcc tgccgctcct ttcgccgacg
     7141 accttttttc ttctgattat gaacatgctg tacgccttct tcgatgtctt tggactgata
     7201 gatattatga ccaaaggtgg tccgggcgac gcgaccgagt tgctggtcta taagctttac
     7261 agggatggtt tcataagcat gaacaccggt ttcgcttccg cgcagtcggt cgtcctcttc
     7321 gtcttcgtcg ccattctgac cgtcctccaa ttcaaataca ccgaaagaaa agttttctac
     7381 ggataacggg tggggatgga taaacatgaa gaagaaaaat cttaaaaaaa tggtgatata
     7441 cctggtgctc accggcatgg cattggtggt gatgttcccg atctactacg ccttcagtat
     7501 gagcacgctc acggcagccg attcctacag ctacccaccc cgtttcacaa ctggaaagaa
     7561 cataatagag aactacgtga cggcctggaa gactgtgaac atgggcagac ttatgctaaa
     7621 cagcggtttc acggccttca tcgtggcgac cggaaagata ggcttcgcta tactggcggc
     7681 cttcgccttc acttacttcg gcgacttcaa gggaaagttc ttcttcttcg gcatgatact
     7741 gataacacac atgcttccgc ttcctgtgag gattttaccg acttacgaac tgatgaagag
     7801 cttcgactgg ataaatacct actacgctct cacaataccc ttctttgcca gcgccacggg
     7861 gacgctgctc ttccgccagc tctttatgac cgtcccgaca tcgctctcgg acgccgcgag
     7921 aatagatggt gccggtccta tgaggtttct cttcaatgtg ctccttcctc tttcaaaaac
     7981 caatatgggc gcgctttttc tgatagagtt caacttcatc tggaacgagt atctgtggcc
     8041 cctcataata actacaacca acgacatgag agtcgttcag atcggcatca agatgctgct
     8101 ggccagcgaa gcccaggcag cggaatggaa catcatcatg gcaggaacga taacggccat
     8161 gatagtacct ctcataatgc ttttgatatt ccagaagact ttcatgagcg gtttcagttt
     8221 aaaagaagaa aaataaccgg ggagaggctc cccggtttgt ccgctcatct cctcgggtct
     8281 ctcttcagtc cgtcgaaatc aaccggagac ttgttctcat cgattatgta ttatactagg
     8341 attgacccga agctcgcagg ttcttcgggc atccggtaga ttgatataga tccttcatta
     8401 tttggtgcga ggtgtgcgag gtttgaggga gtcgtgggaa tattacaacg atatcgcaac
     8461 tagatacgat tacatgtacg aggagcctta ctggcaactt tatcacaggc tcgtagaaag
     8521 actggtggac gatcatatag ctcgccgctc cagggtacta gatctcggca caggcactgg
     8581 aagatgggcc ttgaggctgg ccgaagacgg ccacgacgtc attgccgtag atccggccgg
     8641 ggagatgctg aaagtggcaa agatgaaagc cgaactggcc gagctgaaga tcgagtttca
     8701 tgaggtcggc ggagaggacc ttccttttga aaaggcttcc ttcaactgtg tgctggcaat
     8761 gggagatgtg ctttcttacg ctatggagcc ggaaaaggtt ttaaagaaaa tcggcgaagt
     8821 actaaggaaa gggggcaaac tccttgccag tgtcgataat gcctgggcct tcctccagga
     8881 cttcctctcc cttgcagatt attcgatggc ccagaaattg ctggacgaga gaaagattcc
     8941 gatcggcgac agatcggtga gcaacaagag ctttctttca cgtgcttact ttcctggaga
     9001 gataagcggc atcttaagat ccaacggctt cgaactggtc gatatggctt ctctgatcgc
     9061 cttttatccc tacaacgaag tttcgctggc gtcgaacatc tccagagcct gcgagtggga
     9121 gtaccgttat tgcaggcaga aagagacctt ctccaggtcc gagcacctat tcttctgtgc
     9181 aatcaagagg tagaaacatg gcgaagagac gtaaacaatc accggcaatg ataattggat
     9241 tgacactgat actggcatcg atagttggat ttgtcttctc gttgatggca gtgaaccgtg
     9301 ttgggcaggt caggaaagag atgaccgaac tgaagaattc ttacgagaga aagatagaga
     9361 agatggaggc cgatttccag accaagttag ccagtataga gagattgctt ggagggggtg
     9421 gcacgctaga gaaatacata gtggccaaaa atttcctttc caataccagc gtagatctag
     9481 acgctatact gactgaaatg cagcatcaga gcgacagacc ctatttcaga gtcttcgtga
     9541 ctggcaccaa ggaagtctgg atagggatga aaaagagctc gagcgattcg gcatactact
     9601 tccagaaaaa cttcaaaccg ggtctgtctc aagagaagtt ctactacctt aaaaatcccg
     9661 agatagagac caaatacacg ataatgctga acaaagacgc ctacataagg accggagtgc
     9721 ctgagtcggt ctatctaata ttcttcggct tcgattcggg aaaactctcc agaatgccgg
     9781 gagtagaaat cgccaacctg aaagatgcct tcaatctata catccctgga gggtaattct
     9841 cttctgcctt cacgcgccgg ggtcttgata ttgaaggtaa gaggaatgag cgtgaaaatc
     9901 tttcactctt cgtaactgtt atctctggcc tctctcatcg cacaactcgg aaatcttgct
     9961 aattattatg aacagtaacc cttcgagcct ttgtgatata ataaatatag ccgataaata
    10021 acatcggata tcgtgagaga aaggaggaat aactatgaaa aggttcgaag gaaaagttgc
    10081 gatggttacg ggtggaggtt ccggaatagg tgccgatgcg gctgaagcct tcgcgcatga
    10141 aggtgcgacg gtggttgtca ccgatatcga tgaggagaag ggtagaggac tcgtagaaaa
    10201 gataaacggc catggcggca gggcgatctt tttgaagcac gatgtttcca gcgccgaaga
    10261 gacggaaaaa gtcgttaaaa caatcgtcga gaaattcgga aggctcgaca tagccttcaa
    10321 caacgcaggc ataagcggcc cttccgtttc ggtagccgag tatcccgttg atacatggga
    10381 aagaataatc tccataaacc tgagcggggt cttttactct atgaaatacg aagtcgaaca
    10441 gatgcttaag cagagtggag gagccataat aaacaactct tcgattctcg gaaaagtcgg
    10501 cttcagcaac gcttcggcat atacggcctc caaacacggc gtggttggac tgacaaaagc
    10561 ggcggcgctc gaatacgcca gcaggaatat cagaataaac gcgatcaacc cggccttcat
    10621 caagaccccc ctgctggaag cggccggtat gggccaaacc tcgccggcct acgagatgat
    10681 ggtggccctc catccggtcg gtaggttcgg agaacccaga gaagttacca gtgtcgtgct
    10741 ctttctagcc agcgacgagg catcattcat acacggggag tcgctgatgg ttgacggcgg
    10801 gtacacggcc agataatcga ccgttgaggt aacatatgtt actgacctct ctttcacgct
    10861 gaagtatcat ctgatttaga agacggaggt gtgaaaatga gcgaactgtt caacagggaa
    10921 gattttctca agaatcttat aaagagaata cacgatgatc cggaaaacct cgagaaagtg
    10981 aaagaggatt tcatgagagt cgtcagggaa atgactccta tagagattgc aaagatcgag
    11041 caggaactcg tcaacgaggg tatgcccgca gagagtatcc agctcatgtg caacattcac
    11101 ctggatgttt tcaaagaggc gctcagtgaa gatgagatcg acgttgagcc gtggcaccct
    11161 ctccacatac tggtcgagga acatagagac atcctcaaca ggtcgaaaga actgcgcgat
    11221 cgagcaggcc gtataagaaa cggagatcta acgcagagcg acgtccaggc catagccaat
    11281 ctgctcgact acatggacga ggtagagaaa tatttcctca aggaggagaa cgtactcttt
    11341 ccctatctgg aaaagcacgg actggtacag ccgcccgcga tcatgtggaa ggaacacgat
    11401 gaggttcgtg gattgcgcaa aaagctgaga gagatgatag acgacggcac agtcgaaccg
    11461 gcaaaggttt tcgatctttc gataggtatc ggggagatct tttccaatca catcttcaaa
    11521 gagcacaaaa tactcttccc ctcggctttg aaactgctct cactggaaga gtggcgcgac
    11581 atcaggatac agttcgacgg gataggctat ttcgcatatc gaccgatgcc tgtggaattc
    11641 tcactttcga atcgaaaatg gaagcctcca gtagggaagg ccttttgaac ctgggtagcg
    11701 gctacctgac tttcgatcag ctcagagcga tgatgagctc tttacccttc gacataacct
    11761 ttgcggacgg cgacgacact gttcgtttct tcagtgaagg accggacagg atctttgtga
    11821 gaacgcgtgc ggtcatagga cggaaagttc agaactgcca tccgcaaaaa agcgtgaacg
    11881 tcgtcaacaa aatcctcgcc gatttcaaag ccggccaacg tgatgtcgca gacttctggc
    11941 tcaacctcgg cccaaaaatg gtatacataa aatacatagc cctcagggat tcacagaaaa
    12001 ggtacatagg aacgctcgaa gtgacacagg atatcgctcc tctaagggag ctggagggtg
    12061 aaaaaagaat ctacgacgct ctctaggagt tgatcataaa tcgggtcgaa aatccggggc
    12121 agatattgta gaatataatg tggctttcaa gaatcttcta tattcttccg gaggtataaa
    12181 atggttcgca gagaaatggg aaagaccgga gaaaaggtat ctgccctggg gttcggatgt
    12241 atgaggttgc ccacgctcga cagcggcata gatatacccg aagcgacgcg tatgttgaga
    12301 cacgccatag ataacggggt cgattatgtc gatacggcct ggggttacca caacggccag
    12361 agcgagccgt tcgtaggcga agccctcaaa gacgggtaca gggaaaaggt gaatcttgcc
    12421 acgaagcttc ccagctggct cataaagagt cacgaagaca tggatcacta tctcaacgag
    12481 cagctcaaga gactccagac agatgttata gacttctacc tacttcacgc cctgaacggc
    12541 agattctggg acaaccttgc gaagaacaac gtcttcgact tcatggacag ggcaaaaagg
    12601 agcggtagaa taaagcacat cggattttcc tttcacgata cgctggagac tttcaagaag
    12661 atcgtcgatg cgtacgactg ggagttctgc cagatacagt acaactttct cgacaccgac
    12721 taccaggccg gaaccgatgg gctcaactac gcggccgaaa gggagatggg tgttatagta
    12781 atggagcctc tcaggggcgg taaactcgtc aggcgtatcc ccgcagatgt gcggaagata
    12841 tgggatttgg ctccacagaa gagatcgccc gtcgagtggg cactaagatg ggtgtggaac
    12901 gatccggctg tcggggttgt gctaagcggt atgtcgagca tggaacaagt ggagcagaat
    12961 ctggagatag caggcgcggc ccttccaaat tcattgacct ctcaggaact ctcccttata
    13021 gccagggtca gagaaatata cataagccgg ataaagatca attgcaccgc ctgcgggtac
    13081 tgcatgccct gtcccaacaa cgtggctata cccgattctt ttgaggttta caacgatgcc
    13141 gcgatgttcg acgatgtgga aggtgcgaga aggtcttaca gcaacttctt caaacccgag
    13201 aacagagcat ccatgtgtgt cgaatgcggt agatgcgagg agctctgtcc gcagaaaata
    13261 cccataatag agaagctaaa agaagtggcc gcacttctcg aataacggta cagagtgtga
    13321 cttccggagt cacactttta ttttaccggg ccatcgaatg ttaactatgc tgctatataa
    13381 ttatgcggga atactagtga aagaaaagag cgcagacgat ctgaactcaa ggggggatat
    13441 tgagtgactg gaatagttct tgccgcaggt ctcggcaaga gaatgaggtc gaacctgccg
    13501 aaagtcgcac atatgattat cgatcggccg atggttaact gggtggtctc atccctgaaa
    13561 gaggccggag tcgacagggt ggtggtcgta accggttaca gagcggagat aattgaatcg
    13621 cttctggaaa gcgatatact gaccgtcaga cagaatgaac aactgggtac cggacacgct
    13681 gtcatgatgg ccaaagattt tctcgacgac gacgatgtcg tgattgtggc tggagacgaa
    13741 ccgttgataa agccttcgac tcttcgggag atggtccgca ggaggagaga ggccgatctg
    13801 gacgtgcttt ttctcacgat ggttccgcct gatccttccg gatatggcag ggttgtgaag
    13861 cgtgacggaa aggttgagat cgtcgaagag agagatgcca gcgaagagat caggaatata
    13921 aaggaagtga acaccggcat ttacgccttc cggggagctt tcataagtaa ctcgatagta
    13981 acccttaaga acagcaacga ccagggggag tactatctga ccgatacggt cggcattgcc
    14041 ggcagagccg acaccttggt tgtagccgat gccgaggagg ttctcggtat aaacaacagg
    14101 gcacagcttg cacaggtagc gagaatcgcc agaaagagga taaacaacac tctaatggaa
    14161 gccggtgtga cgatagtcga tccagaaact acatacatag gaccgggagt gaagatcggt
    14221 atcgacagcg tagtagaacc catgaccttt atttatggta agaccacgat cggtcagaac
    14281 tgtcttatag gaccgatgac aaggatacag gattcaaaaa ttgcagacaa tgtcgaaata
    14341 gtacgctcgg aagtactgga agcagatgta agggaaggag ccagggttgg gccttattcc
    14401 cgtctgaggc cgggagcggt gatcatggaa caggctcatg tcggcaacta cgtggaactc
    14461 aagaaatcga tcctcggcaa acgatcgaaa gccaaccatc tcacatatct gggcgacact
    14521 atcataggtg aagatgccaa tataggcgct ggaacgatca catgcaacta cgacggcaag
    14581 aacaaattca aaaccagtat cggcgacagg gccttcatcg ggagtaactc ctcgctggtt
    14641 gcgccggtcg agatcggtga agggtccgtc acggcggcgg gctcggtcat aaccgacgat
    14701 gtccccccgt actcgctggc gctcggaagg gccagacaga tcaacaaaga gggtaagtat
    14761 aaaaagaaca gagaggagaa ggactgatgc catatcagac gaatgaaatg aagatctttg
    14821 cgggttcggc caatctcgct ctgtcgaaga gcattgccga ctatcttgga gttcaccttg
    14881 gagactgtca cacg
//
LOCUS       DAOOLY010000025        14219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4606_length_14219_cov_5.314742, whole genome shotgun sequence.
ACCESSION   DAOOLY010000025 DAOOLY010000000
VERSION     DAOOLY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 14219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14219
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_14219_cov_5.314742"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..682)
                     /locus_tag="PLD15_02415"
     CDS             complement(239..682)
                     /locus_tag="PLD15_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HPB62781.1"
                     /translation="MNPAIFSIGVVISTLFWWITGLSAGGIVTPVYLFIFIDQPLRLV
                     YTWAVGLLTFIILNFLQRYLILYGRKRLALGITMGVFVKLALDTLLVPNLPLELFSTV
                     IGTVVPGLIANDFYRQGVVKTSLSLAFVTLLVWVLNISLKGLALL"
     gene            complement(679..1785)
                     /gene="pgsB"
                     /locus_tag="PLD15_02420"
     CDS             complement(679..1785)
                     /gene="pgsB"
                     /locus_tag="PLD15_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HPB62782.1"
                     /translation="MSNLILSLVAFLTLLTVLIVEKLVLRRRRKRMKIVVQVNGTRGK
                     SETVRLIHGVLSGNGYRVLAKTTGTVPLWIMPDGSHREIKRRGPANIQEQYRALKQAG
                     RLKCDALVVECMAIRPEMQLASGKILDADLTVITNAYPDHIEEIGASEEATAGVLSLS
                     VPGNRKCLVGNLGEGALEMMRTHCERVGSELLRAGERTISDEGMAFRPHPDNLRLIDR
                     VIEVFGLNEKASMEGMARVLPDVGTFRYLKLKLNSGSAILANAFAANDIRSTIYLLER
                     TVADFPGRRVVGFFNSRDDRPDRALVFEPFTRKFGNLMIRGSIPVHKFKGLKYRKVKD
                     GESIEEFLQKGDIVFGFGNIRGLVQWLSNLEVIE"
     gene            complement(1782..2888)
                     /gene="pgsW"
                     /locus_tag="PLD15_02425"
     CDS             complement(1782..2888)
                     /gene="pgsW"
                     /locus_tag="PLD15_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HPB62783.1"
                     /translation="MKSHSFVSLSGLNKAGVSLKWLSVGVVIGLVSFFLLTSSVRRSE
                     TPFISTQLAAASLMREATREISSYRLSLGIEIDPLLDPNGTGLIGPEFTELTTTLGNL
                     EAKRTSTNPDFAALIVKYFRELGLKKGDPVAIGASGSFPSLVLATLCACEAMNLEPLL
                     IYSIGASEHGATHPEFTFVRMLQRLVEKGLLRDSLIAVSLGGNNDNARGMFFPGAVEK
                     MTEIALSTGKTFIREDTLQKSIDARFKIYFEYAGGLPEVFVNVGGASPNFGESPAALS
                     LENGLITSLKNIPVGEDRGLIFEYASRGVPSIHLLNIRDLALKNGIPIDPVPLPEPGK
                     SGVYYIDSYSLPLALFFLALMVLSLIAGKLAVKK"
     gene            complement(3089..3844)
                     /locus_tag="PLD15_02430"
     CDS             complement(3089..3844)
                     /locus_tag="PLD15_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HPB62784.1"
                     /translation="MFEEISEIERAIAGLVDGAKVAMIHSSLSAMGRVHGGPAAVVEA
                     VARLAERGITIMMPALSYEHVTPERPLFDLERTPSNVGAIPEAFRKSEGVVRSVHPTH
                     SVCAFGPLAQVVTAEHEKDRTPVGERSPFRKLVEKDGVVIFIGCGLRPNTLMHGVEEL
                     VKPPYLFNGEVSYTIVTGKGSEYTAGYLTHDFKGYVQRYDRIAGFLTKNEMRTGRVLK
                     AECYALRAVPIWEKALKEIKKNELAFVDRTLEG"
     gene            complement(3895..5058)
                     /locus_tag="PLD15_02435"
     CDS             complement(3895..5058)
                     /locus_tag="PLD15_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPB62785.1"
                     /translation="MTQLLIIGVVIGAGIALGRSMKPLRLPAVTGYVFAGVLIGLFFK
                     PSGETELILSTVSSSALAFIAYTMGTSLNLRELKKMGGGVIIVALTQAFGATLVTILT
                     LTGLLGQPLELTLILGAIASTTAPAATLMIVRQYRANGPFTRTLLPVVALDDAIAIII
                     FGFSQIAVASMVSGKAIAAGLFLEPLATLGISAAIGFAGGLLLSYLARKATDEDSLLA
                     ITLGVLFVTVGITEYFGYSALLAGMVLGTVVTNLSSRNMAILNGIDRISYSLVISFFV
                     FAGMGLNLELLPRLGLVGLAYVVARSVGKIFGAWIGGVSTRREPAVRKYLGLALLPQA
                     GVALGLAASFDAGSPELAEKVRTIIVSAVIVFELIGPPLAKIALKLSGDIKEN"
     gene            complement(5065..5409)
                     /locus_tag="PLD15_02440"
     CDS             complement(5065..5409)
                     /locus_tag="PLD15_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPB62786.1"
                     /translation="MELLVFVLGKVDLLDRVVKSLLEAGVKGLTILDSTGVSAMAESG
                     ILKDLPVISSLKFLVGERRPFNKTLFSVVENETAGKAIEAVRAIVDINEPGAGILFSV
                     PVSNVFGGSFQV"
     gene            5810..6217
                     /locus_tag="PLD15_02445"
     CDS             5810..6217
                     /locus_tag="PLD15_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62787.1"
                     /translation="MRTRLIAVFSILLLFLTTGALAYTLEDAQSVADRANTVVETLIA
                     NAEKTAEVLTENYEKFVERFKDNESLLALLEESYNRSIYALGTSLAVAAFRVSSAAID
                     KIAMMGYKAECYYVSITLGNQVFQIDPIIIIDD"
     gene            6356..7255
                     /locus_tag="PLD15_02450"
     CDS             6356..7255
                     /locus_tag="PLD15_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB62788.1"
                     /translation="MSGFFVRKSGEAIERIVQKSGSLSLLGQGDGVEILIQEIKEGSL
                     SYLEPYGEELLEFYYIIEGEIYCHCEDGETVLKPGDYFYAHNLKAPVEIKPLTDAKLI
                     YVSSRALFKHISHNISVFKEILKKVEEKDEYTHGHGKRVRDVSHAIGKKLGLDSRKLE
                     RLAMGALFHDIGKIDIPDEILKKPARLNPEEFELIKKHPVYGYKMVLNTPLEDTSDII
                     RHHHERVDGSGYPDGIPGELITIESKIIAVADSFDAMTSVRPYREAMTDDEALREIKS
                     LSGTLYDPKVVQALEECLREGLI"
     gene            complement(7326..8513)
                     /locus_tag="PLD15_02455"
     CDS             complement(7326..8513)
                     /locus_tag="PLD15_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HPB62789.1"
                     /translation="MGVKISESVTWVGKIDWELKKFHGEDYSTHRGSSYNSYLVRDEK
                     VALIDTVWSPFGEEFVEKLKKEIDLSKIDYIIANHGEIDHSGGLPALMREIPDTPIYC
                     TANAVKSLKGQFHEDWNFVTVKTGDTLKLGKKTLTFVEAPMLHWPDSMFEYLDGEEIL
                     FSNDAFGQHYATELLYNDLVDQAELYQEAIKYYANILTPFSKLVTRKIEEVLSFKLPL
                     KMICPSHGVIWRKDPAQIVKKYLEWAGDYQENQVTIVYDTMWDGTKKMAEAIAMGIHQ
                     TDGTVTIKLFNAGKSDKNDIVTEIFKAKAVLFGSPTVNKGILAPLSGMLHEIKGLAFK
                     KKKAAVFGTYGWSGESVDVLNAFVKDAGFEVIQEGLKKLWSPDETNLKDCEQFGKEFA
                     AKL"
     gene            complement(8661..9836)
                     /locus_tag="PLD15_02460"
     CDS             complement(8661..9836)
                     /locus_tag="PLD15_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPB62790.1"
                     /translation="MRRLLFLSSVVLFLLSGCLPIFSPWSWTSRVRGSFQFSSPAMGG
                     KGIVYAASQEDLYAFTQTGKSTSIYGLGSGEWFGGNPSVDEYGNIYIGTTGGRLLKIS
                     PEGLLLWSFPSQPSGNYTVRDTSIDGEGNLYFGTTGGTMYCVSPEGVELWHYDTGEEI
                     WSKPAIGEDGTAFFGTLVTGGSETHSFFAIKDGQPLWVFETEAPSVGFYSSPALDGEG
                     NVYVACHDGYLYCLDTLDGTLDWSLRLAPAKPGGDSAIASSPVVGQDGTVYLCTNEGE
                     FYAVSPAGSIQWTASLGTTIFSTPVLSSEGVAYIVGWDDSKLYVVDHDGGVAKRSSMG
                     RNSACSPLMTDDGTLYFTATLVDIGTWSQLVSLETDGTPAGLWPMYGMDQRRTGSLD"
     gene            10039..12282
                     /locus_tag="PLD15_02465"
     CDS             10039..12282
                     /locus_tag="PLD15_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPB62791.1"
                     /translation="MGRKILLFLTVLIVSLVFAGEFRSIEAIKASGELRILQTEDYWY
                     PFYYLDEEGKLGGIDVELGRDIAAALGVKPLFVRAVATHETLATHLREGEGDIILSYY
                     SYSPSRASQVYASRDYLVQNLSLLINNQVLQKIKNEVDELTPEKLIENLNDPSISVLV
                     MIGTVYERIALQLLPGTKIFSIGTTENLAGLLFEKNYDVFFMNDLWTETFLVKNPSLL
                     IHYSIYRLPSEDQIVVGVNPANTDLLEWINRFLDSDYNKTREYLNRYVDYELIAKLHR
                     ETAEESRIKLLLEDPYRWIVLFVILLLAFLVVWSSQKKIAKKSENGKAPHWLLNIWTI
                     LFAMVLGFYSGGAFPDIVEGLSPLGELFFNYMLLCGLPILFCVVTLNILRLLMKSGGG
                     GFLLKFLGVVLVFFVLGALIGIVVAMIVEPGTGVAKESQEALVVSMKATNPFEEVNVT
                     TGSILWSLPTNMISNSIIRAFAENKTLAIVFFSIVLAFAIKFINDSKREVIINSLETA
                     GEIFIFLFKLSYYVLPFGIFSLMLSQASVFTGDTSVMKSLIMLIGCQLLCFVIWLVVS
                     IALGAKLAKMSPLAFLGAIKKPTMLLFALMSSVAVIPEALHTSERVRQFNDENQRGVL
                     PLLVVMLQPHTVSLFAVVAIFNLQLLGRSLGPVDYLYIAISSIIAVIATIGVPSPLDL
                     FTISMVVLPFNIPPTQAIFFMLPWMYTGRRLDLMGMMINDFAAVQLFRSKKPKEESPS
                     PVAEVHE"
     gene            12360..>14219
                     /locus_tag="PLD15_02470"
     CDS             12360..>14219
                     /locus_tag="PLD15_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HPB62792.1"
                     /translation="MKLSLFFGPTASGKTETILSQLEKVHRTDPFSYYFVGPSGDHVR
                     YFRENFVSRVGTINSSRFLAMDQFAVDIFRLLNPASYHISDYIIRLEIGNILEKMGKR
                     ELIDSAMFIDYILEMIHDVKEQGGFTEIFASDDEAVSTLIDIYRALQERFAQNTIFDT
                     FDAYTGIEERADEIHKGEFGRYLFIDGFHDFSPAMSKFFGSVFPSFEEIFITIPDDPG
                     REELYSEVASIRRFIEDFEKRTPDIEVSRIFLKERYYPVKLEHFMSNLFCENKRAGLC
                     PAVEVTVFPDLFSEVEWTARLVKKYLVDGYEPGEISLVASDFQIYDRLLSQKLREYGV
                     PWRSEGDEPLLSSLAVRKLILPLEAAVLGFPPDKLIAMGDNGYGGEIDARFLEMAATM
                     SRIIYDRAHVRLKLEERRTSWVERLEKFIEFTSRRRDAVLSLAEDDLESAPALEMEET
                     IRRVREDLLPAVGRIFDKLRPFKSMRMRPVGEYSQMFAGWERDLAIIDSYRKLEQNDG
                     EDELRALKEFFDRVLPELERVLLFMNRPRIAPAEYYSYLMLMLRKESYPASRSIANRV
                     EIQSLLKSRFSKRKIKIFLGFVDGAYPYVKLNPLYSFTQYSSLRPKDLLLTREK"
BASE COUNT         3346 a         3875 c         3681 g         3317 t
ORIGIN      
        1 gaaaatgggg actgacgatt cacctcacgt ctgttgtacc caattttcgc gctcaaggaa
       61 cctggacgcg cagcgtcgga acgataaacc gctcttcgca ctttacgtcg cgtgcggaga
      121 acgacgggcg gatgacggag aacgtctccc aacgcaacga aggacgggtc catgctcttg
      181 gacgacagac gctggacggc tctttccact cttcgcggcg ttcagtcttt gcccgcgttt
      241 atagcagagc cagccccttg agagatatgt tcaagaccca cacaaggagg gtaacgaagg
      301 ccagcgaaag cgaggttttc acaacgccct gccggtagaa atcgttggcg atcaaacccg
      361 gtaccaccgt acctataacg gtcgaaaaca gttcgagagg caaattcggc accagaagag
      421 tatcgagagc cagtttcacg aagactccca tcgttattcc cagggcgagc ctctttctac
      481 cgtagagtat caggtaccgc tggaggaagt tcagaatgat gaacgtcaaa agccccacgg
      541 cccaggtata gacgagccgt aacggctgat ctatgaatat gaagagataa accggcgtta
      601 cgatgccccc cgcggagagg cccgtgatcc accagaaaag tgtactgatc accactccta
      661 tcgaaaatat ggccgggttc attcgatcac ctccagattg ctcaaccact gtaccagacc
      721 tcttatgttg ccaaagccga aaactatatc gcccttttgc agaaactctt ctatcgactc
      781 gccgtcttta accttcctgt acttcagacc tttgaacttg tgcaccggga tcgaacccct
      841 gatcatcagg ttgccgaact ttctggtgaa gggctcgaag accagtgccc tgtcgggccg
      901 atcgtctctg gagttgaaga accccaccac tcttctgccg gggaagtccg ccacggtcct
      961 ctccagaagg tagatcgtgg aacgtatatc gtttgcggcg aaagcgttcg cgagaatcgc
     1021 gctgccgcta tttagcttca actttaggta cctgaacgtg ccaacgtcgg gcaaaactct
     1081 cgccatacct tccatcgaag ccttttcatt cagcccgaaa acctcgataa ccctatcgat
     1141 cagacgcaga ttgtccggat gcggtctgaa ggccattcct tcgtcggata tcgtcctctc
     1201 tccggccctg agcagctcgc tgccaactct ttcacaatga gttctcatca tttccagggc
     1261 cccctcgccg agattgccga caaggcactt tctgttgcct ggaactgaaa gcgagagcac
     1321 cccggcggtt gcctcttcgc tcgccccgat ctcctctatg tggtcgggat aggcgttcgt
     1381 gatcacagtg agatcggcgt ccagtatctt tccgctggcg agttgcatct cgggtctgat
     1441 cgccatgcac tctacgacga gcgcgtcgca cttcaatctc ccggcctgct tcagcgccct
     1501 gtactgctcc tgtatgtttg ccgggcctct cctctttatt tcgcgatgac tgccgtcggg
     1561 cattatccag agcggcaccg tgcccgttgt cttggccagc accctgtaac cgttccccga
     1621 cagcacgccg tggataagtc tgacagtctc gctctttccc ctggtaccgt tgacctggac
     1681 aacgatcttc attctctttc tccgccgtct gaggaccagc ttctcgacga tgagaaccgt
     1741 cagcagtgtg aggaacgcaa cgagcgaaag aatcaggtta ctcatttttt caccgccagt
     1801 ttgcccgcga taagcgacag gaccataagc gccaggaaaa acagcgccag gggcagcgag
     1861 tagctatcga tataataaac gccgctcttt cccggttccg gaagcggcac aggatcgatc
     1921 gggattccat ttttgagggc cagatcgcgg atgttgagca ggtgaatgga tggtactccg
     1981 cgagaggcgt attcgaaaat caagccccta tcctcgccca cggggatatt cttgagggaa
     2041 gttataagcc cgttctcgag gctgagggcc gcgggactct ctccgaagtt cgggctggcg
     2101 cctccgacgt tcacgaagac ttccggaagc ccgccggcat attcaaagta gatcttgaac
     2161 ctcgcgtcta tactcttctg aagcgtgtct tcgcgtatga aagtctttcc ggtcgaaagg
     2221 gcgatttccg tcattttctc gaccgccccc gggaaaaaca taccccgggc gttgtcgttg
     2281 ttgccgccaa gagagacggc tattagactg tcccgcagaa ggcctttctc aacgagtctt
     2341 tgaagcatcc tgacgaaggt gaactccgga tgggttgccc cgtgttcaga ggctccgatc
     2401 gagtaaatca ggagaggctc cagattcatc gcttcacagg cgcagagggt tgccagcacc
     2461 agcgacggga aagaaccgct cgcgcctatg gccaccgggt ctcccttctt caaacccagt
     2521 tccctgaagt acttgacgat cagcgcggcg aaatccgggt tggtcgatgt cctcttggcc
     2581 tcgagatttc caagcgtcgt agttagttcg gtgaactcgg gacctatgag tcccgtgccg
     2641 ttcggatcga gcagcggatc tatctctata ccgagcgaaa gtctgtagga agagatctcg
     2701 cgagtggcct ccctcatcaa gctggcggcc gcgagctggg tgcttatgaa gggcgtctcg
     2761 cttcttctca ccgaagaggt gagaagaaag aaggaaacca atcctataac gactccgaca
     2821 gagagccatt tcaaagaaac tccggccttg ttcaatccgg aaaggctcac aaaagaatga
     2881 cttttcaaga taactcccct ttaattcaac taacggtcag gactcttatt atcgctatca
     2941 gcactccgcc caaagccgcc tgcctccgtc tccttataat gatcaattat acattacctc
     3001 cggaaaagct tcccggagaa tgaaggcggc ccggcctctc gcggcgaact ggaaatatct
     3061 tcctttcaag tattaccggc ggatatctct acccttcgag tgttctgtcg acaaaagcca
     3121 gttcgttttt cttaatctct ttgagcgctt tttcccagat cggaacggcc cttagcgcgt
     3181 agcattcggc cttcagtact ctccccgttc tcatttcatt tttcgttaga aatcctgcga
     3241 tcctgtcgta cctctgaacg taccccttga agtcgtgggt gaggtaaccg gcggtgtact
     3301 ccgatccttt cccggtgacg atggtgtacg aaacctcccc gttgaaaagg taaggcggtt
     3361 tcactagctc ctcgaccccg tgcatcagtg tgttcggcct caatccgcat ccgatgaaga
     3421 tcacgactcc gtctttctcg acaagctttc tgaagggcga tctctcgccc accggagtcc
     3481 tgtctttttc gtgttcggcc gttacgactt gcgcgagcgg accgaaggcg cagaccgagt
     3541 gggtaggatg cacgctcctg acgaccccct cgctcttcct gaaggcttcg ggaatggccc
     3601 cgacgttgga aggcgttctc tccagatcga agagaggcct ttccggtgtt acgtgctcgt
     3661 acgaaagggc cggcatcatg atcgtgatcc ctctttccgc gagtctcgcc acggcctcaa
     3721 cgactgccgc gggaccgcca tgaacacggc ccatggctga aagcgacgag tgaatcatcg
     3781 ccactttcgc accgtcgaca agtccggcga tggcccgttc tatctcgctt atctcttcga
     3841 acaactacac catctcccca ttttgagtgt gaaaggcgag gcgtgacctc actttcaatt
     3901 ttcctttata tctccggaca gtttcagagc gattttggcc agaggcgggc cgatcagttc
     3961 gaacacaata acggccgaca ctattatggt cctcaccttt tcggccagct ccggagagcc
     4021 tgcgtcgaaa ctcgccgcca accccagggc aactccggcc tggggcagca aggccagccc
     4081 gagatacttt ctcaccgccg gttcgcgtct ggtggagaca ccgcctatcc atgctccgaa
     4141 gatcttgccg acgcttctgg caaccacata cgcgagacct accaggccga gcctgggcag
     4201 caattccaga ttgagtccca ttccggcgaa gacgaagaag cttatcacca gcgaataaga
     4261 gatcctgtct ataccgttca aaatagccat gttcctgctg gagagattgg tgacgaccgt
     4321 tccgagcacc ataccggcaa gcagggccga atagccgaag tactccgtta tgcccacagt
     4381 tacgaaaaga actccgagcg taatcgccag aagactgtct tcatccgtgg ccttcctggc
     4441 gaggtacgaa agcaggagcc cgccggcgaa acctatcgcg gcagatatgc cgagcgtggc
     4501 cagaggctct aagaagagcc ccgcggcgat cgcctttcca ctcaccatac tcgcgacggc
     4561 aatttgagaa aaaccgaata tgattatagc tatggcgtcg tccagggcaa cgacgggaag
     4621 gagcgtcctc gtgaacgggc cgttggccct gtactgcctc actatcatga gagtcgcggc
     4681 cggagcggtg gtcgaggcga tagccccgag tatcaacgtg agttccagcg gctgaccgag
     4741 caggcccgtg agagtcagaa tcgtaaccag cgtagctccg aaggcctgtg ttaaagcgac
     4801 gattatgact ccgccgccca tctttttcag ttcgcgcaga ttcaacgatg tacccatggt
     4861 gtaggctatg aaagcgagag ccgaactgga cacggtagaa agaatcagtt ccgtttcgcc
     4921 ggacggtttg aaaaacagac cgatgagcac cccggcaaaa acgtaaccgg ttacggcagg
     4981 cagtctgagc ggtttcatag atctgcccag cgctattcca gcccctatta ccactccgat
     5041 tatgagcagc tgcgtcattt tcaatcacac ctggaaggaa ccgccgaata cgttcgagac
     5101 cggtacgctg aagagtatcc cggctccggg ttcgtttata tccacgatgg cccttaccgc
     5161 ctctatggct ttgccggccg tttcattttc cacaacgctg aacagagtct tgttgaaggg
     5221 acgcctctcg cctaccagaa acttcaaaga ggatattacg ggaagatctt tcaggatacc
     5281 cgattctgcc atcgccgaga cgcccgtgct gtccaggata gttagaccct tcacgcctgc
     5341 ctccagaagg cttttcacaa ctctgtcaag cagatcgact tttccaagaa caaaaaccag
     5401 gagttccaca aactccacct ccgttcaatt cagttttatt ttagaaattc gagatgtgcg
     5461 aagatattcg attgatcagc tcaagtgtac tatagaaaag cataccgggt atgactgaaa
     5521 tggtaagcgg tagttgaaaa tcgaagcgat catggggaac ctccggtttg atactttcaa
     5581 tatcgatatt ctcttatttt gttcaatcgc acaaagataa gttgagctat aatacagctg
     5641 taagaacagg ctttttctaa cgcacatatc acaaacacgg atttcgcggc gagagatttt
     5701 tcgatggggc cgatcggtta gtgagcgata aaaaaaagta cttttaaatc tgttgatttt
     5761 atgatatgat ttatacacac gagtaaataa cggaaaagag gtggcagtta tgcggactcg
     5821 gttgatagcg gttttctcca ttttattgct cttcttgaca acgggcgccc ttgcatacac
     5881 cctcgaggat gctcagtcgg tggcggacag agctaacacg gtggttgaga cgctgattgc
     5941 caacgcagaa aagaccgcgg aagtcctcac agaaaattac gagaagttcg ttgagagatt
     6001 caaagacaac gagagtttgc tggctcttct ggaggaaagt tataacagat cgatctacgc
     6061 tctgggtacc agtcttgctg tagcggcctt cagagtcagt tcggcggcca tcgataaaat
     6121 cgcgatgatg ggatacaagg ccgaatgtta ttatgtttcc atcacccttg gcaaccaggt
     6181 tttccagatc gatcccataa taatcatcga cgactgagtt caatagtttc aactcataca
     6241 ggccgcgcag taatcgcggt ctttttttgt ttgaggcgca ctgatgccga tatctatgct
     6301 aaactctata tagatggctg gaaagaaata tcgattttac tctgtcggag gaattatgtc
     6361 aggttttttc gtgagaaagt ccggagaggc gatcgagaga attgtccaaa aaagcggctc
     6421 actttccctt ctcggtcagg gagatggcgt agagatactg atacaggaga ttaaagaggg
     6481 ttctttaagc tatctcgaac cttacggaga ggaacttctc gaattctact acattatcga
     6541 aggcgaaatt tactgtcact gcgaggatgg cgagacggtt ctgaaaccgg gagattattt
     6601 ctatgcacac aacctgaaag caccggtcga aatcaagccg ctgactgacg caaagctcat
     6661 atacgtctct tcgagagctc tttttaagca cataagccat aacatatccg tgtttaagga
     6721 gatactcaaa aaagtcgagg agaaagacga atacacgcat ggccacggaa agagggtcag
     6781 ggatgtttct cacgcgatag gcaagaaact cggtctggac tccagaaagc tcgaacggct
     6841 cgcgatgggc gcgcttttcc acgatatagg caagatagat atacccgacg aaatactcaa
     6901 aaaacctgcc agattgaacc ccgaagagtt cgaactcata aagaaacacc cggtatacgg
     6961 ctataagatg gtattgaaca caccgctgga ggacacatcc gacatcatcc gacatcatca
     7021 cgagagagtc gacggctcgg gatatcccga cggtattccc ggagaactga taacgatcga
     7081 atcgaagata atcgcggttg ccgattcttt cgatgcgatg acttcggtca gaccatacag
     7141 agaggccatg accgacgatg aagcccttcg ggagatcaaa tcgctctccg gcaccctcta
     7201 cgacccgaaa gttgtccagg cgctggaaga gtgcctccgc gaaggtctta tctagcaccg
     7261 tcttacccgc acacaattag gaaaaaataa agggccgatc atccggcccc ttgtttatct
     7321 atgttttaga gcttcgcagc gaactctttg ccgaactgct cacagtcttt gaggttggtc
     7381 tcgtcaggag accatagctt cttgagcccc tcctgaatca cttcgaaacc cgcatctttg
     7441 acgaacgcgt tgaggacatc gaccgactcg ccgctccagc cgtaagttcc gaaaacggcc
     7501 gctttctttt tcttgaaggc caggcccttt atctcgtgca acattcccga gagcggtgcg
     7561 aggatccctt tgttgacagt gggggagccg aacagtacgg ccttcgcctt gaagatttcg
     7621 gtcactatgt cgtttttgtc gctctttcca gcgttgaaaa gctttattgt cacggttccg
     7681 tcggtctggt ggatgcccat tgcgatcgct tcggccatct ttttcgtacc gtcccacatc
     7741 gtatcgtaga cgatggtcac ctggttctcc tggtaatctc cggcccactc gagatacttc
     7801 ttgacgatct gggcggggtc ctttctccat atcactccgt ggctgggaca gatcatctta
     7861 agaggcagct tgaaactgag tacctcttcg atcttgcgcg taacgagctt gctgaaagga
     7921 gtgagtatgt tggcgtagta ctttatcgct tcctggtaca gctcggcctg atcgaccaga
     7981 tcgttgtaga gaagctcggt cgcgtaatgc tggccgaagg cgtcgttact gaagaggatc
     8041 tcttcgccgt ccaggtactc gaacatcgaa tcgggccagt gcaacatcgg ggcttcaacg
     8101 aacgtgaggg tcttctttcc gagcttgaga gtatccccgg tcttaacggt cacgaagttc
     8161 cagtcttcgt ggaactgccc cttgagggac ttgactgcgt tggccgtaca gtaaatcggc
     8221 gtatcgggga tttcacgcat cagggcgggc agtcctccgc tgtggtctat ctctccgtgg
     8281 ttggcgatta tataatcgat cttcgagaga tcgatctcct tcttcagctt ttctacgaac
     8341 tcttctccga agggcgacca taccgtatcg atgagggcaa ccttctcgtc ccgcacgaga
     8401 taggagttgt aactggaacc cctgtgtgtc gaataatcct cgccgtggaa cttcttcaat
     8461 tcccaatcaa tctttccaac ccaggttacg ctttcgctta ttttcactcc cataacaaca
     8521 cctcccgtgt atttcgattt aattctacct cttttgacgg cattgagcgg taacatttgt
     8581 tacactcttt tccggccttt cagaatcaaa gaaaaatacc acgaaaacgg aacggcaagg
     8641 gtttcatgac gcaaagatct tcagtccaga cttccggtgc gtctttgatc cattccgtac
     8701 atgggccaca gtcccgcggg ggttccatcg gtctcgagag atacgagctg gctccaggtg
     8761 ccgatgtcca cgagcgtggc ggtgaagtac agcgtgccgt cgtccgtcat gagcggagag
     8821 caggcgctgt tgcgccccat gctggagcgt tttgccactc ctccgtcgtg atctacaacg
     8881 tacagtttcg aatcgtccca gcccactata taagccacgc cttcggagga caggaccggg
     8941 gtggaaaaga tcgtggtgcc gagggaggcc gtccactgta tcgatccggc cggagagacg
     9001 gcgtaaaatt ccccttcgtt ggtgcaaaga tagacagtcc cgtcctgtcc tacaaccggg
     9061 ctggaggcta tggcgctgtc tccgccgggc tttgccggag ccagtctcag actccagtcg
     9121 agcgtgccgt ccagagtgtc caggcaatag agatatccgt cgtggcatgc gacatatacg
     9181 ttgccctccc cgtccagcgc gggactggag tagaaaccaa ccgacggagc ctcagtctcg
     9241 aaaacccata agggctgccc gtctttgatc gcaaaaaaac tgtgagtttc actgccgccg
     9301 gtgacaagcg tgccgaagaa ggcagttccg tcttccccta tcgccggctt ggaccagatc
     9361 tcttcacccg tatcgtaatg ccagagttcc actccctcag gcgatacgca atacatggtg
     9421 ccgccggttg ttccgaaata gaggttgccc tcgccgtcga tcgaagtatc gcgaacggtg
     9481 taatttcccg aaggctgtga cggaaaggac cacagcagaa gcccttcggg ggagatcttg
     9541 agcagccggc cgccggttgt tccgatatat atgtttccgt attcgtcgac agaagggttg
     9601 ccgccgaacc attctcccga tcccagaccg taaatcgaag tggacttacc ggtctgcgtg
     9661 aaagcgtaaa gatcctcctg cgaggcggcg tacacaatcc ccttgccgcc catggccggg
     9721 ctgctgaact gaaaggagcc cctgactctg gaggtccagc tccagggcga gaagatcggc
     9781 aaacagccgg aaagaagaaa aagaacgacc gacgaaagaa acaacaacct gcgcatctgg
     9841 caacaactcc ctgcgaaaaa aataagaacc ggtactgaga ttatatcaat ctccggagcg
     9901 gtaactgaat aaaaacatta ccaagatcgg cagctatttg ttggagaagt gtatagccta
     9961 atgtctttct gtgctcttca taactatctg taaatgctat aattctcgat attggcggaa
    10021 aaggcggggg gagaccaaat gggcagaaag atcttgcttt tcttaacggt tttaatcgtt
    10081 tctctggttt tcgcgggtga attcaggagt atagaagcta taaaagcctc cggcgagttg
    10141 aggatcctcc agactgaaga ctactggtat cccttctatt atctggacga agagggaaag
    10201 ctcgggggaa tcgacgtcga actggggagg gacatcgcgg ccgcgctcgg agtgaaaccg
    10261 ctcttcgtac gggccgtcgc gacacacgag accctggcca cacatctgag agaaggggag
    10321 ggcgacataa tattgagtta ttactcttac tccccctcca gagcctccca ggtttatgcc
    10381 tcgcgcgatt atctcgtaca gaacctgtcg ctgctgataa acaatcaggt gcttcagaag
    10441 ataaaaaacg aagtcgatga gctcacgccc gaaaagctca tcgagaacct gaacgatccg
    10501 tcgatctccg tactggtgat gataggaacg gtttacgaga ggatcgccct ccagctcctc
    10561 cccggaacta agatattcag tatcgggacc acggaaaacc tggccgggct tctcttcgag
    10621 aagaactacg acgttttttt catgaacgat ctctggaccg agacctttct ggttaagaat
    10681 ccctctctgc tgatccatta ctccatttac cgtctccctt cggaggacca gatcgtggtg
    10741 ggggtcaacc ccgccaacac cgatctgctc gagtggatca acaggtttct ggacagcgat
    10801 tacaacaaga cgcgagagta tttgaacaga tacgtcgatt acgaactgat cgccaagctc
    10861 caccgagaga ccgcggaaga gagtcggata aagctgctgc tggaagatcc ctacaggtgg
    10921 atagtgctct ttgttatcct cttgctggcc tttctggtcg tatggagcag tcagaagaag
    10981 attgccaaaa agagtgaaaa cggcaaggct ccccactggc tgctgaacat ctggacgatc
    11041 ctcttcgcga tggttctcgg gttctactcg ggtggcgcct tcccggacat cgtcgaaggt
    11101 ctcagcccgc tgggtgaact cttcttcaac tacatgttgc tctgcggcct tccgatactg
    11161 ttctgcgtgg tcacgctgaa catcctcagg cttctcatga aatccggggg cgggggcttc
    11221 cttttgaagt tcctgggggt ggtactcgtc tttttcgtcc tcggagcttt gatcggaata
    11281 gtcgtggcga tgatagtcga gccgggaaca ggggtcgcca aagaatcgca ggaggcgctg
    11341 gtggtatcca tgaaggcgac taaccccttc gaggaagtga acgtaacgac cggctcgatc
    11401 ctgtggtctc ttcccaccaa catgataagc aacagcataa tcagggcctt cgccgagaac
    11461 aaaacgcttg ccatagtgtt cttctcgatc gttctggcct ttgccattaa attcatcaac
    11521 gactccaaga gagaagtgat aatcaattca ctggagacgg ccggagagat cttcatattt
    11581 ctcttcaagc tttcctatta cgtacttcct ttcggcattt tcagcctcat gttgagtcag
    11641 gcatcggttt tcaccggaga caccagcgtt atgaaatcgc tcataatgct tatcggctgt
    11701 cagcttctct gtttcgtcat ctggcttgtc gtctcgatcg cgctgggggc gaaactggcg
    11761 aagatgtcgc ctcttgcgtt tcttggagcc ataaagaagc cgacgatgct tctcttcgct
    11821 ctcatgtctt cggtggcggt aatccccgaa gcgcttcaca cgagcgagcg ggtcaggcaa
    11881 ttcaacgacg aaaaccagag gggagtcttg ccgcttctgg tggttatgct ccagccgcac
    11941 accgtttcgc ttttcgcggt cgtggcgatc ttcaacctcc agctgctcgg tcgcagcctc
    12001 ggtccagtcg attatctcta catagctata tcttcgatca tcgcggttat tgcgacgata
    12061 ggagttccaa gtccgctgga cctcttcaca atttccatgg tcgttcttcc cttcaacatc
    12121 cccccgacgc aggcgatctt cttcatgctg ccatggatgt acacgggaag gaggctggac
    12181 cttatgggta tgatgatcaa cgattttgcg gcggtacagc tcttcagaag caagaaaccg
    12241 aaagaagaaa gcccgtcccc ggttgccgaa gtccacgaat gaagggcgca gaaaagaatc
    12301 ccgcccggcc ctttcagggt tgcaggtgat aaaattgtat cgctgacagg gggtgttcca
    12361 tgaagttgtc tttgtttttc ggaccgaccg cttcgggaaa gaccgaaacg attctctcac
    12421 agctcgaaaa agtccacagg accgatccct tttcttatta cttcgtcggt ccgtcgggcg
    12481 accatgtgcg ttatttcagg gagaatttcg tgtccagggt tggcacgatc aactcctcca
    12541 ggtttctcgc catggatcag ttcgccgtcg atatattcag attgctgaat cccgcctctt
    12601 accatataag cgactacatc ataagactgg aaatcggaaa catcctcgag aagatgggca
    12661 agagagagct tatagactcg gccatgttca tagactacat actcgagatg atccacgacg
    12721 tgaaggagca gggaggtttc accgagatct tcgccagcga cgacgaggcc gtctcgacgc
    12781 tgatagacat ataccgggcc cttcaggaga ggttcgcgca gaacaccatc ttcgacacct
    12841 tcgacgctta caccgggata gaggagcgcg ccgacgagat acacaaaggc gagttcggca
    12901 ggtacctttt catagacggt tttcacgact tcagcccggc gatgtcgaaa ttcttcggct
    12961 ccgtcttccc ctccttcgag gagatcttta taactatccc cgacgacccc ggcagggagg
    13021 aactgtattc ggaggtcgcc tctatcagga gatttataga ggatttcgag aagcggactc
    13081 cagatatcga ggtgagcagg atcttcctca aggagaggta ttacccggtt aaactagaac
    13141 atttcatgtc taatctcttt tgcgagaata aaagggccgg actctgcccg gccgtggagg
    13201 taacggtctt tccggatctc ttctcggagg tggagtggac cgccagactc gtaaagaagt
    13261 acctggtcga tggctatgaa ccgggagaga tttcgctggt cgcctccgat ttccagatat
    13321 acgacaggct cctctcacag aagttgcgag aatacggcgt cccctggagg agcgaaggcg
    13381 acgagccgct gctctcttca ctggccgtca ggaagctcat actgccgctt gaggctgccg
    13441 ttctcggctt tccgcccgac aagcttatag ccatgggcga caatggctac ggcggggaga
    13501 tcgacgccag gtttctcgag atggcagcca ccatgtcgcg aataatatac gacagggccc
    13561 acgtcagact gaaactggaa gagaggcgca cttcatgggt agagcggctg gaaaagttca
    13621 tagagttcac ctccagaagg cgggacgccg ttctctcgct ggccgaagac gatctggaga
    13681 gcgcgccggc tctggagatg gaagagacta tacgcagggt cagggaggat ttgctgccgg
    13741 ccgtcgggag aatcttcgat aagctccggc ccttcaagag tatgcgcatg aggccggtcg
    13801 gggagtactc gcagatgttc gccggctggg agagggatct ggccataatc gattcgtaca
    13861 ggaaactcga acagaacgat ggcgaggacg aactccgggc tctgaaagag ttcttcgaca
    13921 gggttctgcc cgagctggaa cgcgttctgc tgtttatgaa ccgtccccgg atcgctccgg
    13981 ccgagtacta ctcatacctc atgttgatgc tcaggaaaga gagctacccg gcctcgcgca
    14041 gcatagccaa cagggtcgag atacagtcgc tcctcaagtc gcgtttctcg aagaggaaga
    14101 tcaagatctt cctgggcttc gttgacggag cgtaccccta cgtcaaactg aatccactgt
    14161 acagcttcac gcagtacagc tcgctccggc cgaaggatct ccttctcacg cgcgagaag
//
LOCUS       DAOOLY010000026        14104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4657_length_14104_cov_5.214392, whole genome shotgun sequence.
ACCESSION   DAOOLY010000026 DAOOLY010000000
VERSION     DAOOLY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4657_length_14104_cov_5.214392"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="PLD15_02475"
     CDS             complement(<1..2012)
                     /locus_tag="PLD15_02475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB62793.1"
                     /translation="MKYEVGRFTENLKEVASKVDKYEIDLEEAVKALRRFGFVEDDYS
                     ALEYLTGEMTNSSRLFELGRSHNAYIRVLAYIRLGGMLPPDGLSKFIKSLELERLAIT
                     ALMRGMARNGRTLPVETLYFLFGMDLEIDELLLETVELKDRETNSAFLLESSMLRGNS
                     LKFTGDMEEELMKGGKRGALALDYLDFHNVNWLEYLNRSNLSDPTISMKAILKAVRRG
                     IVPEFLSKCRFNWADNLYRSLEKLRGVHTAGGSLIQFSFHGDPFKSGQGSSGGIGTFL
                     RTLGNELADILDSVVTVVPLEETELDDNLQLYRVEKDGHLFLYFPFYGYQKLKAAHFI
                     GSQWSLASTIDDILSIAGISPAYFHTRFSDYASLSMMKLARIKGIKTHFTLTVDPQRR
                     FSGNGGTLLETGIEKIMEDTSRVQISWRMLRESDSLIGIGARQSERQLISYYPSLIKE
                     DLRSKLNMVPEGISLRDECPAEIRHRYTGYNSLFEVERKYHIERDRKTLPVILTVGRL
                     DPLKGQFKLLKAWGESPLFNRYNLVIVGGSVEDPDEVEAGELRKIEEYIRKYPRLSTG
                     FCHLSAMGNDYIRCLEKDIAVSQGPRPPVYAAPSFKEEFGIAILEAMAAGFLAIAPRR
                     GGVKEYIKHGLNGFLIDTTSVETLGRDLVKILLDKDGNPIEIKPYER"
     gene            complement(2110..4524)
                     /locus_tag="PLD15_02480"
     CDS             complement(2110..4524)
                     /locus_tag="PLD15_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPB62794.1"
                     /translation="MKNIKTNTGVGKWLPRKFDLEKAAGTLQFADDLRFGPELLHAAV
                     VRSSIPHGEILSIDTAQALEMPGVVKVLVGEDFPHHFGLYLKDRTPMAIGRTRYCGEP
                     VALVVAEDENTAREAVKRVRVNYKKLEAIFDPVKAATTNTVLVHPELHKYEHVDFITP
                     QPNTNIGNWFRIRKGDAEKAFGEADHIFEETVTCPQIAHGFLETLCSICKQDPASGEI
                     TIWTSAQSAFTVRDIIAKGIGYPLHKIRVVAPPIGGGFGGKAGMTIEALCLAAAMDKN
                     IAGRPVKLFIPREEVLLTSWVRQGFVAKIELAVNNDGLIQAIRNTFWFDTGISAEYGA
                     NPVRSAGYTSMGAYNIPNVWSDCYAVYTNKPFGGAYRGFGLPELMSALEVVIDIAADK
                     LHIDPVEFRKKNLLRKGQTTCTGMPMHDHALDKIIDRVAASLKISQKEPPLRKGWKRG
                     KGIALAIKAPAIPADASSSAIVKLNGDGTVEVLAATMDMGQGTYSAYAQIVSEELGVP
                     YDWIKVYYPDTQSHPYDWQTVASRSCWSMGMAVKRAACDVREKLIALFSEYWQVSCDS
                     ISIEYGVIKCRKLGKNERIDERVQNGFKMPDGVLKGGPIIGTGSFTPPDIIYPDIDTG
                     QSAKSVVHFTLGAIGVDMEVDPGTGKIVINRAVAGYDVGKAITPINVRGQIEGGTVQG
                     LSACLMEAMAYDENGKLLTPDFTDYKIATFKDAPAEIEAFWEETPEEISPYGNRGVGE
                     HSMIAPAPATNNALFNATGMRIHSYPLSRERVFKALHAKERGETDFWEWPYALDLAFR
                     KAKKEW"
     gene            complement(4526..5014)
                     /locus_tag="PLD15_02485"
     CDS             complement(4526..5014)
                     /locus_tag="PLD15_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB62795.1"
                     /translation="MRDRKIQLNLNGRSVSLVAGDNTTLLHALREYGLTSLKRGCEEG
                     ECGACTVIMNGLPEKSCLILVAEAEGADILTVEGLIKDGRLHPIQQSFIDEGAIQCGY
                     CTPGMVMTTYALLLKNPSPDEEEIKEALSGNLCRCTGYLSIVRAVKKSARIMAGLSEG
                     SE"
     gene            complement(5011..5889)
                     /locus_tag="PLD15_02490"
     CDS             complement(5011..5889)
                     /locus_tag="PLD15_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HPB62796.1"
                     /translation="MKMRDFVLERPQSIPEALDILKKAGGRAMVKSGGTDLIVWLHRS
                     LVNPDCVIDLTKIEQLKGIGLSNGRIEIKALATLNEVASNPAVGEKLPALFQACMAHS
                     DPLIRNRATVVGNVCSAVPSGDVIAPLLCYGAMVEITGPSGDRTLRIGDFITGPKKTA
                     LAADEIVTAIKIALPEGKSAGCYIKISRREALDIAQAAVCCTAFESVTREFRISFCAV
                     SPRPVRATEAERLLNGATKMDDGIVEAAAKLAIEAVDPITDVRASREYRLAMIEELTR
                     RAIKSCVEKLEAGGPS"
     gene            complement(5911..6702)
                     /locus_tag="PLD15_02495"
     CDS             complement(5911..6702)
                     /locus_tag="PLD15_02495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPB62797.1"
                     /translation="MELKNWDKIRIYELSQPISHLTPPWPTYEPLQIKFFKRLAPNGA
                     NGQLLTHSNHVGTHLDGSLHFCTHGRDIASIPLNELVAPGVVVDLSDIAEDYGIYTSK
                     DIEDRVEVREGDILIINTGYHRYAFDQPEADEVRYMIKHPGPTREFADWCRKKKLKWI
                     GVDCGSADHPMNTKIRDWMPVQAKECDAHFRKKYGKTLEEIFPPDDYQLMHVLLFPYD
                     IIHAENVGGEIDQILNRRMIIGCFPWRFVGGESCISRIVAFDEQE"
     gene            complement(6719..7981)
                     /locus_tag="PLD15_02500"
     CDS             complement(6719..7981)
                     /locus_tag="PLD15_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPB62798.1"
                     /translation="MVTLFDIEKANQTAFERLQKAEPHIVDLGIACEVIPGMEKNTIL
                     HAGPPIDLERMSGPMMGAVTGAVIYEGWADTPQKAIEYLKEKNLAFDPCHHHSAVGPM
                     AGIISPGMPVWIVEDGSSGKRSYATLNEGLGKVLRMGAFDQEVIERLHWMREVLFPVL
                     KAGVERYVEENGGINLKSLISQALHMGDELHNRNRAATSLFIRSLVPYLVESSTDNTS
                     LAKVIRFMAGNDHFFLNPGMAAAKVSLDGVGSVEGSTMVTVMARNGTDFGIQIAGLER
                     RWFTCQAALPDVLLFPGFTRADVNRDIGDSAIMETYGVGGFALAAAPAIVQFVGGTPQ
                     DAINYTREMYEICLGENRNFTIPSLNFRGTPTGIDLIKVVETGITPILDTGAAHREAG
                     RGQVGAGIVRMPSEAFVSAATAFTDRYL"
     gene            complement(7965..8144)
                     /locus_tag="PLD15_02505"
     CDS             complement(7965..8144)
                     /locus_tag="PLD15_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62799.1"
                     /translation="MSMKKLLKDGPKVINMGMEQFYIDLKAREVPAVRVDWKPPLADR
                     ELLGKLKKLRGGNFV"
     gene            complement(8141..9676)
                     /gene="fdrA"
                     /locus_tag="PLD15_02510"
     CDS             complement(8141..9676)
                     /gene="fdrA"
                     /locus_tag="PLD15_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HPB62800.1"
                     /translation="MKRLEVVKGEYYDSVTLMLVAKELKKIEGVAEATLNMATEANLH
                     IMKTAGFEVDGVNASPDDLIIGIDCPGELFDGLMETARSYLSSPPWKGGEEPSEYTPR
                     TLEGALSVLPEADIALISLPGRYAAAEAMKALERGLNVMLYSDNVSLEEEIELKRYAS
                     RNGLIVMGPDCGTVVINGEGLGFSNACPVGPVGIVSASGTGLQEVMVQLARRGAGVKH
                     GLGTGGRDVKSAVGGISSLIAIEALANDPEISVVVVIGKPPSTEVREKLVQTLRSCGK
                     QSVTCFMGESPRKDEPQLKYTTGLEECATVVVALLDGKDAGKAREDLMNEYLKIEALG
                     RKSETSGRFLRGLFTGGTLCYEAESIARRYLESIYGNNPLKIGMKLPDSLKPIQNSFI
                     DYGEDEFTQGKLHPMIDPSFRTGQLKMQSEDPSVAVVLFDVVLGYGSAPDPSSEVVGA
                     IKGLERKRGPLYVAYVCGTSGDPQCLERQIAILKEAGVRVFESNARAAIFAAAATVGR
                     ERR"
     gene            complement(9715..10407)
                     /locus_tag="PLD15_02515"
     CDS             complement(9715..10407)
                     /locus_tag="PLD15_02515"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HPB62801.1"
                     /translation="MITLYPFERSSDWPKTMIVMKVHSVYSKAINFEGENNRRYSLIT
                     GEPDYLPRAALIEALPFLKAGETVKISPELSSVGFDPSIDPGSESVNPLWQPLWREWK
                     RFLLDDRLDCLLDHLENPEEMLGLGPGTTPAGDDFITGLVTAFRWSGVEVNERLVKAL
                     EKNGTTWFSAQMIRDALNGYIWKRGKKLCQALTGSSASELLSAAGRILQWGHLSGRAW
                     LAGFSTGLEGIS"
     gene            complement(10404..11594)
                     /locus_tag="PLD15_02520"
     CDS             complement(10404..11594)
                     /locus_tag="PLD15_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPB62802.1"
                     /translation="MKLPKPLEGLFVLDLTRVLAGPFCTMLLCDMGATVVKVERPDGG
                     DDSRSFSPMIGDESAYFMSINRGKKSITLNLKSPEGKGLFERLVGKADVLVENFKPGV
                     MKKLGFPYERLMELNPSLIYTASSGFGHSGPMSQRTAYDLIIQGLSGMMSITGPDRDH
                     PTKTGSSIADIFCGTFACIGILAALHNRYNTGEGQMVDVAMLDSMVAVLENAIARFSA
                     TGKIPEPIGNSHPSIAPFASFATLDGQINIAVGNDSIWHKFCTAIERPELCEDPKFST
                     NPERVRNITELTQIISVTLAEKTTRDWLDIFQELDIPSGRINNIADLFNDPQVLAREM
                     IVDVPQKGFNVKMPGVPIKFSATPAGIGGPAPSLGEHNGEIYMKLLGLSFEELEEFKN
                     RGVI"
     gene            complement(11591..12481)
                     /locus_tag="PLD15_02525"
     CDS             complement(11591..12481)
                     /locus_tag="PLD15_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPB62803.1"
                     /translation="MKRTVVSAVQFAIEPMNVEENLSRARRWITKCKEFSGADLVVLP
                     ESFTTGFTPIGGVEDLWKVVSTIPGPLTDIAVQWARELDMYICFPTYERGEVFPVIYN
                     SAALVGPEGLLGVYRKTHPFPSERLEAGGWTTPGSESFSVETPIGNIGVVICYDGDFP
                     ELARVTALKGAEMICRPSAFLRTFDQWELTNRARAYDNHVYWIGTNSVGRDASGAYFF
                     GCSMIVHPSGMKLSQAGGCDEFTYAELDPDPIKKIIPGSNRDQTFDHIQDRNTEAYTG
                     ILEEGKSVFEPARRIPFRRR"
     gene            complement(13132..13533)
                     /locus_tag="PLD15_02530"
     CDS             complement(13132..13533)
                     /locus_tag="PLD15_02530"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPB62804.1"
                     /translation="MGSDLLIVVTAAIAQRNGMVLIARRLDKGKLPGKWEFPGGKTET
                     GEGLEQCLKREIKEELGVEINIQGHFGTSIYEYPFGKIRLEAFSVEFISEEFELKDHS
                     EVRWICVHELDVYDFSPADLPFVESLKDSQR"
     gene            complement(13628..>14104)
                     /locus_tag="PLD15_02535"
     CDS             complement(13628..>14104)
                     /locus_tag="PLD15_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB62805.1"
                     /translation="VQIERIKAPSILASGFIFASYSRLPVDIVAIDHVTILYMDRTGI
                     ISICGNNERFLVNLLGDMGDRLTTLAEKVWLLSLNTISQKLAHFLLKKASENGPEFEL
                     KITREELADAFSVARPSLSRVFGEFTSGGIISQDGKKIKILDENTLKRIMGEPADL"
BASE COUNT         3097 a         3883 c         3426 g         3698 t
ORIGIN      
        1 cgttcatatg gctttatttc aatcggattt ccatctttgt ccagaagtat tttgaccaga
       61 tcccttccga gggtttctac ggaggtggta tcgattagga atccattcaa accgtgcttt
      121 atatactctt tcacaccacc tctgcgtgga gctatcgcta aaaaaccagc cgccatggct
      181 tcaagaatcg ctatgccgaa ctcctcttta aaagagggtg ctgcatagac tggtggccgt
      241 ggaccttgcg agaccgcgat atctttttcg agacagcgta tgtaatcgtt tcccatggcc
      301 gagagatgac agaaccccgt cgaaagccgg gggtacttac gtatgtactc ctctatcttt
      361 ctcagctctc cggcttctac ttcatcgggg tcctctacac tgcctcctac gatgacgagg
      421 ttgtatctgt tgaagagcgg ggattctccc caggccttaa ggagtttaaa ctgtcctttg
      481 agcggatcca gtctccccac cgtgagaata acgggcagag tttttctgtc gcgctcgatg
      541 tggtactttc tttccacctc gaacagcgaa ttgtatccgg tatatctatg gcgtatttcg
      601 gcaggacatt catctctcaa ggatattcct tccggtacca tgttcaactt agagcgtaga
      661 tcttctttta tcaaagatgg gtagtaagaa atcagctgac gttcgctctg ccttgctcct
      721 attcctatca aactatcact ctctcttagc attctccagg agatctggac ccgagaagta
      781 tcctccataa tcttttcgat tccagtctca agcagagtcc ccccgttacc ggagaacctt
      841 ctctgagggt cgacagtcag cgtgaagtgg gtctttattc ctttgattct tgcaagtttc
      901 atcatggata gcgaagcgta atcagaaaac ctcgtatgga aatacgccgg agagattcca
      961 gctattgaaa gaatgtcatc tatcgtcgaa gccagcgacc attgagaacc gatgaaatga
     1021 gcggctttca gcttctgata accataaaac ggaaagtaga ggaataagtg gccgtctttt
     1081 tcgaccctat agagctgtaa attatcatcc agctccgttt cttcgagggg tacaacggta
     1141 actacgctat ccaaaatatc ggcaagttcg ttcccgagag ttctgagaaa agtcccgatt
     1201 ccgccactgg agccctggcc tgatttgaaa ggatcaccgt gaaaagaaaa ctggatcagt
     1261 gatccaccgg cagtgtgaac gcctctcagt ttttctagag aacggtaaag gttgtccgcc
     1321 caattgaacc ggcatttcga aaggaactct ggaacgattc cccgcctgac cgctttgagt
     1381 atggctttca tcgagatagt gggatcggaa aggttggagc ggttcagata ttccaaccag
     1441 tttacgttgt gaaaatcgag ataatccagt gccagagccc ctctctttcc ccccttcatt
     1501 agctcctctt ccatatctcc cgtgaacttt agactatttc ctctcagcat ggacgattcg
     1561 agcaaaaagg cggaattggt ctcacgatct ttgagttcca cggtttccag tagcagctca
     1621 tcaatctcga ggtccattcc gaaaaggaag tagagggttt cgaccggaag tgttctgcca
     1681 tttctcgcca ttccacgcat gagggccgtt attgcaagcc tttctagctc aaggcttttg
     1741 atgaatttgg aaagtccatc cggagggagc atcccgccca gacgaatgta tgcaagaact
     1801 cttatataag cgttgtggga tcttccaagc tcgaataatc tggaagaatt cgtcatctca
     1861 cctgtgagat actcaagggc ggagtaatcg tcctctacga atccaaatct tctcaaagcc
     1921 tttacagcct cttcgagatc gatttcgtat ttatcgacct tcgaagctac ttctttgaga
     1981 ttttctgtga acctgccaac ttcatatttc acgggtgcac ctccgaatag atgatagcag
     2041 gtccgtatcg aaaatcctct attgtggccg accgttgggc gttgggctgt tactgagaag
     2101 gcctgtcgct caccactctt tttttgcctt tctgaaagcc aggtcgagtg cgtaaggcca
     2161 ctcccagaag tctgtctctc ccctttcctt cgcatggagg gctttgaaaa ccctttccct
     2221 gctcagcggg tagctgtgta ttctcatacc ggtagcgttg aaaagggcgt tgtttgtagc
     2281 gggagccggt gcgatcatcg aatgctcccc gactccacgg ttaccgtatg gagaaatctc
     2341 ttcgggtgtc tcttcccaga aggcctctat ctcggcaggt gcatctttaa aagtcgctat
     2401 tttgtaatcg gtgaaatcgg gagtcagcag tttgccattt tcatcgtagg ccatggcctc
     2461 cataaggcag gccgaaaggc cctgcacagt tccgccctct atctgaccgc gcacgttgat
     2521 cggcgttatc gcctttccca catcgtaacc ggcgaccgct ctgtttatta cgatctttcc
     2581 ggttccggga tcgacttcca tatccacccc gatagctcca agagtgaagt ggaccacgct
     2641 cttggccgat tgtccggtat cgatgtcggg gtagattata tctggaggcg taaatgatcc
     2701 agtacctata attggaccac ccttcaaaac gccgtctggc attttgaagc cattctgcac
     2761 tctttcgtcg attctttcgt tctttccgag ttttctacat tttatgactc cgtattcaat
     2821 acttatactg tcacacgaaa cctgccagta ctccgaaaac aatgctatga gtttttccct
     2881 cacgtcgcag gctgcacgtt tcactgccat tcccatggac cagcaggagc ggctggcaac
     2941 cgtttgccag tcataagggt ggctctgagt gtctgggtag taaactttta tccagtcgta
     3001 cggtacgccg agttcttccg agacgatctg cgcgtaagcg ctgtaagtcc cctgccccat
     3061 atccatcgta gccgcgagga cttcgaccgt tccgtcgcca ttcagtttca ctatagccga
     3121 agaggaagcg tcagcgggaa tagccggggc ctttatcgcg agggctattc ctttgcccct
     3181 tttccagcct ttcctgaggg gtggttcctt ctgcgaaatc ttgagactcg cggcaacccg
     3241 gtcgatgatt ttatcgagtg catgatcgtg cataggcata ccggtgcatg tggtctggcc
     3301 tttccttaga aggtttttct ttctgaactc caccggatcg atgtgcaact tgtcggcggc
     3361 gatgtctatc acgacctcta gcgcgctcat gagttcgggc aggccgaacc ccctgtaagc
     3421 gcccccgaag ggtttgttgg tgtaaactgc gtaacagtcg gaccagacgt tcggtatgtt
     3481 gtaagctccc atagacgtat agccggcgct cctgacaggg ttggcgccat actcggccga
     3541 gatgcccgtg tcgaaccaga aggtattgcg gattgcctgt atcagaccgt cgttgttcac
     3601 cgcgagctcg attttagcca cgaagccctg acgaacccag gatgtgagca gcacttcttc
     3661 tcttggtata aagagtttga ccggtcttcc cgctatattc ttatccatgg cggccgccag
     3721 acagagcgcc tcgatcgtca ttccggcctt tccgccgaaa cctccgccta tgggaggtgc
     3781 tacgactcta attttatgga gaggatagcc gatgcctttc gcgattatat ccctgacggt
     3841 gaaggccgat tgagcagatg tccagatcgt tatctcgccg gatgcagggt cttgcttgca
     3901 gatcgagcac agcgtttcga ggaagccgtg ggctatctgc ggacaggtga cggtctcttc
     3961 gaagatatgg tcggcctccc cgaaggcttt ctctgcgtcg ccctttctta ttctgaacca
     4021 gttccctatg ttcgtgttgg gttgaggcgt tatgaaatct acgtgctcat atttgtgaag
     4081 ctccggatga acgagaacag tgttggtcgt ggcggccttc acaggatcga agatcgcttc
     4141 tagctttttg taattgaccc tgacacgttt cactgcctcc cgtgccgtgt tttcatcttc
     4201 ggccaccaca agtgccacgg gttctccgca gtatctagtt ctgccgatgg ccatcggcgt
     4261 tcgatccttg agataaagac cgaaatggtg agggaaatcc tcgccaacta aaactttcac
     4321 taccccaggc atctcaaggg cctgagctgt atcgatcgac agtatctcgc cgtgcggaat
     4381 agacgagcga acgacggcag catgaaggag ctccggtccg aaccttagat catcggcaaa
     4441 ctgaagagta cctgccgcct tttcgagatc gaactttctt ggcaaccact ttccaacacc
     4501 ggtattggtt tttatattct tcatctcact cgctcccttc gcttagacct gccatgattc
     4561 gggcgctctt ctttacggcc ctgactatcg agagataacc ggtgcacctg cataggttgc
     4621 ccgaaagcgc ctcttttatc tcttcttcgt ctggagaggg gttcttcagc agtaaagcgt
     4681 aggttgtcat aaccattccc ggagtgcagt aaccgcactg tatcgcccct tcgtcgatga
     4741 aggactgctg tataggatga agcctcccat cttttatcaa tccttcgacg gtgaggatat
     4801 cggctccttc ggcttcggca accaggatga gacaggattt ctccggcagg ccgttcatga
     4861 tcactgtgca ggcgccacat tctccctctt cacaacctct cttgaggctc gtgaggccgt
     4921 actctcgcag ggcgtggaga agagtggtgt tgtcaccggc cacgagggaa acggaccggc
     4981 cgttcagatt gagctgtatc tttctatctc tcatgatggt cctcccgctt ccaacttctc
     5041 tacacagctt tttatagctc tgcgcgtgag ctcttctatc atcgccagac ggtactctct
     5101 gctggccctc acgtcagtaa ttggatcgac ggcttcgata gccagctttg ccgccgcttc
     5161 gacgataccg tcgtccatct tcgtcgcgcc gttgagtagt ctttcggcct cagtcgctct
     5221 gacaggacgt ggggagacgg cgcagaatga gattctgaac tctctagtga cgctctcaaa
     5281 agccgtgcaa caaacggccg cctgtgcaat gtccagggct tcgcgacggc ttatcttgat
     5341 gtaacatccg gccgacttcc cctcaggcaa agcgattttg atcgccgtca ctatttcatc
     5401 ggctgcgaga gccgtcttct tgggaccggt tatgaagtcg ccgattctga gcgttctgtc
     5461 gccagaggga ccggtgatct caaccatcgc cccgtagcat agcaggggag ctatgacgtc
     5521 tcccgaagga acggccgaac acacattgcc gacaactgtg gccctgtttc tgatcagagg
     5581 atcggaatgg gccatgcagg cctggaacag tgccggcaac ttctctccga cggccgggtt
     5641 ggaagccacc tcgttcagtg ttgccagagc ctttatctct atacgtccgt tcgacagccc
     5701 tatccctttg agttgttcta tcttcgtgag atcgattaca caatctggat tcacaagtga
     5761 cctgtggagc cagacaatca gatccgttcc gccgctcttc accatagccc ttcctccggc
     5821 ttttttcagg atatccagag cctcgggtat ggattgcggc cgttccagaa cgaagtcgcg
     5881 catcttcatc gccacgcttt caattggatt ctattcctgt tcgtcgaaag ctactattct
     5941 acttatgcag gactctccgc ccacgaatct ccaggggaag caaccgatta tcattctcct
     6001 gttgagtatc tggtcgattt ctccgccgac gttttcggcg tgtattatgt cgtaggggaa
     6061 gagaagcacg tgcatcaact ggtagtcgtc cggcgggaag atctcttcga gagtcttgcc
     6121 gtatttctta cggaaatgtg cgtcacactc cttcgcctga acgggcatcc agtctctaat
     6181 ctttgtgttc atcggatgat cggccgaacc gcaatcgacg ccgatccact tcaacttctt
     6241 cttcctgcac caatcggcga actctctagt tggcccggga tgttttatca tatagcgcac
     6301 ttcgtcggct tccggttgat cgaaggcata tctgtggtat cccgtgttga tgatcagtat
     6361 gtccccttcc ctcacctcga cgcggtcctc gatatctttg gaggtgtata tcccgtaatc
     6421 ctccgctata tctgagagat cgacgacgac tccgggagca actagttcgt tgagcggtat
     6481 gctcgcaata tcccgaccgt gagtacagaa gtggagcgat ccatccaggt gtgttcctac
     6541 atggttggag tgcgtgagga gctggccgtt ggcaccgttc ggcgcgagcc tcttgaagaa
     6601 ctttatctgc aaaggctcgt aggtaggcca aggcggtgtc aggtggctga taggttgcga
     6661 gagttcgtag attctgattt tgtcccagtt tttcaattcc atgagcggtt tccctccttt
     6721 ataaatatct gtcggtgaaa gcggttgcag ccgatacgaa tgcctcggaa ggcattctaa
     6781 ctatgccggc accgacctgt cccctgccag cctcgcgatg ggcggctccg gtatcgagaa
     6841 taggtgtgat gccggtctcc acaactttta tgagatctat acccgtcggg gttcctctga
     6901 aattgagcga gggaatcgtg aagttcctgt tttcacccag acagatctcg tacatctctc
     6961 ttgtgtagtt gatggcatcc tgaggcgtac cgccgacgaa ctggacgata gcgggcgccg
     7021 cggccagtgc gaaacctccg actccgtagg tttccattat cgcactgtca cctatatccc
     7081 tgttcacgtc ggccctggtg aatcccggga aaagcaaaac gtccggcagc gcggcctggc
     7141 aggtgaacca ccgcctttcc agaccggcga tctggatccc gaaatccgtt ccgtttcgcg
     7201 ccataacggt caccatcgta gagccctcga cagaaccgac gccgtccagc gaaaccttcg
     7261 ccgcggccat gccggggttg agaaagaagt gatcgttgcc ggccatgaac cggatcacct
     7321 tcgccagaga ggtgttgtcg gtggaggatt ccacaagata aggcacgagg gagcggatga
     7381 acagggaggt cgcggcccgg ttcctgttgt gcagttcgtc acccatgtga agggcctggg
     7441 agataaggct tttgagattg atacctccgt tctcctcaac gtaacgctcc acgcccgcct
     7501 tgagcacggg gaataggacc tctctcatcc agtgaagcct ttcgatgacc tcttggtcaa
     7561 aggcgcccat tctgaggact tttcccagtc cttcgttgag tgtagcgtag ctccttttgc
     7621 cgcttgagcc gtcctctaca atccatacgg gcattccggg cgatattatt cccgccatcg
     7681 gcccgacggc gctgtggtga tggcagggat cgaacgccaa gtttttttcc ttcaagtatt
     7741 ctatggcctt ctggggtgta tcggcccagc cttcgtagat gacggcgccc gttacggcgc
     7801 ccatcattgg ccccgacatc ctttccagat ctatgggggg tcctgcgtgg agtatcgtgt
     7861 tcttctccat tccggggata acctcgcagg ctataccgag atccactata tgcggttcgg
     7921 ccttttggag cctttcaaaa gccgtctggt tggccttttc gatgtcaaac aaagttacca
     7981 cctctcagct tcttcaattt cccgagcagt tccctgtcgg ccaacggggg cttccaatcg
     8041 accctgacgg caggtacttc ccgagctttc agatcgatgt agaactgctc catacccatg
     8101 tttataactt tcggaccgtc tttcagaagt tttttcatac tcatctccgt tccctcccga
     8161 ccgtggccgc agcagcgaaa atcgccgctc tcgcgttgct ctcgaagact cgaacgccgg
     8221 cctccttcag aatggcgatc tgtctctcca gacactgggg atcgccggac gttccacaca
     8281 cgtaggcgac gtaaagcggt cctctcttcc tctcgagacc ctttatcgca ccgaccactt
     8341 cggacgatgg gtccggcgcg ctgccgtatc cgagaacgac atcgaagaga acgaccgcga
     8401 ccgagggatc ctccgactgc atctttagct gtccggtcct gaaggaagga tcgatcatag
     8461 ggtggagttt accctgagtg aattcatctt cgccgtagtc tatgaaactg ttttgaattg
     8521 gtttaagact gtcgggcaac ttcattccaa tcttcagcgg gttgtttccg taaatcgatt
     8581 ctagatatct ccgagcaatg ctctcggcct cgtaacagag cgtaccaccc gtgaagagac
     8641 cacgcaggaa ccggccggaa gtttcgctct tccgtccaag ggcttcgatt ttcaaatact
     8701 cattcatcag atcctctctg gcctttcccg cgtctttgcc gtctaggagc gccaccacga
     8761 cggtcgcaca ctcctcaaga ccggtagtgt acttcagctg tggctcatcc ttgcggggcg
     8821 actctcccat gaaacatgta accgactgtt tcccgcagct tctcagggtc tggacaagct
     8881 tttccctcac ttcggtcgag ggtggtttac ctataacgac cacgacggag atctcgggat
     8941 cgttcgcgag cgcttctata gcaatcagcg aagagatgcc gcccacggcc gacttaacat
     9001 ctctgccacc cgttccaaga ccgtgcttca cacctgctcc gcgcctggcg agctggacca
     9061 tcacttcttg caatcccgtg ccggatgccg aaactatgcc gacggggcct accgggcagg
     9121 cgtttgaaaa gcccagccct tcgccgttta tgaccaccgt tccgcagtca ggacccatca
     9181 ctatcagtcc attccgggag gcgtacctct taagctctat ctcctcttcc aatgaaacgt
     9241 tgtcggaata gagcattacg ttcaaacctc tttcgagggc cttcatcgcc tccgccgcgg
     9301 cgtaccttcc cggcagagag atcagcgcga tatcggcctc cggaagcaca gacaacgctc
     9361 cttcaagcgt cctgggcgtg tattcgctcg gctcctcacc acccttccac gggggcgagg
     9421 agagatagga tctggccgtt tccatcagtc cgtcgaacag ctccccgggg cagtcgattc
     9481 cgattatcag atcgtctggc gaggcattca caccatccac ctcgaatcct gcagtcttca
     9541 ttatatgcaa attagcctca gttgccatgt tcaacgtggc ctcggcaacg ccttcaatct
     9601 tctttagctc cttcgccaca agcatgagag tcacactgtc gtaatattcg cccttaacaa
     9661 cctctagcct cttcaagaga tcgacctcca tttctctcaa ctatctactc tcgctcacga
     9721 aatcccttcg agtccagtag agaaacccgc gagccaggcc ctgcccgaaa gatgtcccca
     9781 ctggagaatc cttccggccg ccgataaaag ttcagacgct gaactgccag ttagagcctg
     9841 gcagagtttt ttgcctctct tccagatata tccattcaaa gcatctctta tcatctgagc
     9901 ggaaaaccag gtcgttccgt ttttttccag cgcctttacc aaccgttcgt tcacctctac
     9961 ccccgaccat ctgaatgccg ttacgaggcc ggtgatgaaa tcgtcgccgg caggtgtcgt
    10021 tcccgggccg agacctagca tctcttcggg attctcaagg tggtccagca ggcaatcgag
    10081 ccggtcgtca agtaaaaaac gcttccactc ccgccagaga ggttgccaga ggggattgac
    10141 agactctgat ccaggatcga tcgaaggatc gaacccaacg ctggaaagct ccggagagat
    10201 cttaacagtt tcaccggctt ttaaaaaggg gagggcttct atgagtgccg ctctgggtag
    10261 ataatccggt tccccggtga tcagagaata cctcctgtta ttttctccct caaaattgat
    10321 agccttcgaa taaacgctgt ggaccttcat tactatcatc gtcttcggcc agtcggagct
    10381 tcgttcgaaa gggtacagag tgatcatatg actcctctgt ttttaaactc ctctaactct
    10441 tcgaaactca aaccaagtag tttcatatat atctctccat tgtgttctcc gagcgatggg
    10501 gccggtccac cgattcccgc tggcgttgcc gagaacttta tgggcacgcc cggcatctta
    10561 acgttgaatc ccttctgggg aacgtccacg atcatttcgc gcgcaagtac ctgtggatcg
    10621 ttgaagagat cggcgatgtt gtttatacga ccggacggta tgtcgagttc ctggaagata
    10681 tcgagccaat ctctggtggt tttctctgca agggtgacag agattatctg tgtgagttcc
    10741 gttatatttc tcacgcgctc tggattggtg gagaacttag gatcttcgca caactccggc
    10801 cgctctatcg ccgtacagaa cttatgccat atactgtcgt ttccgacggc gatgtttatc
    10861 tgaccatcga gcgtcgcgaa cgaagcgaag ggtgcgatcg acggatggct gttgccgatc
    10921 ggttcgggta tctttcccgt cgccgagaat ctggcgatcg cgttctcgag aacggccacc
    10981 atgctatcca gcatcgcgac atctaccatc tgtccttcgc cggtattata tctgttgtgg
    11041 agcgccgcca gaataccgat acaggcgaag gttccgcaaa agatatcggc aatagagctc
    11101 ccggtcttcg tagggtggtc tctatcggga cccgttatgc tcatcattcc gctcaatccc
    11161 tgtattatca gatcataagc cgttctctgg ctcatcggtc cggaatggcc gaaacctgat
    11221 gatgccgtgt agataagcga ggggttgagt tccatgagtc tttcgtaggg gaaaccgagc
    11281 ttcttcatca cgcccggttt gaaattctcc accagcacat cggcctttcc tacgagtcgc
    11341 tcgaagagcc cctttccctc gggagatttc aggttcaacg ttatactctt ctttcccctg
    11401 ttgatactca tgaaataagc gctttcatcc cctatcatgg gactgaaaga gcgggaatcg
    11461 tcaccgccgt cgggccgttc gaccttcacc acggttgccc ccatatcgca gagcagcatt
    11521 gtgcaaaaag gtcctgcgag gactcttgtt aaatcgagta cgaacaatcc ttcgagtggc
    11581 tttggcaatt tcatctcctc ctgaatggaa tgcgtctggc cggttcgaag actgatttac
    11641 cctcttcgag aattccggtg taagcctctg tgttcctatc ctgaatatgg tcgaaagtct
    11701 ggtctctgtt cgagccggga atgatctttt ttatgggatc tggatccagt tcggcatagg
    11761 tgaactcgtc gcagcctccg gcctgagaga gcttcatgcc tgatggatga acgatcatcg
    11821 agcagccgaa gaaataagcc ccgctggcat ctcttccgac tgaattggtg cctatccagt
    11881 aaacgtgatt atcgtaggcg cgtgctctgt tggtgagctc ccactgatcg aatgttcgca
    11941 gaaaagcgga aggcctgcat atcatctccg ctcccttcaa ggctgtaact ctcgccagtt
    12001 cggggaaatc accgtcgtaa cagattacca cacctatgtt gcctatcgga gtctcaaccg
    12061 agaaggactc actgccagga gtggtccatc cgcctgcctc cagtcgttcc gatggaaagg
    12121 gatgagtctt tcgatagaca cccaacagac cttcggggcc gaccagtgcg gcgctgttgt
    12181 agatcaccgg gaagacctcc cccctctcgt atgttggaaa gcatatatac atatccagtt
    12241 ctctggccca ttgaacggct atgtcggtca gcgggccggg aatagtcgaa actaccttcc
    12301 acaggtcctc gacaccgccg ataggagtga aaccggtggt gaagctctcg gggagcacca
    12361 caagatctgc tccagagaac tctttacact tcgttatcca gcgacgggct ctcgaaaggt
    12421 tctcttcaac gttcatcggt tcaatcgcga actgtacggc agaaacaacg gttctcttca
    12481 tatctactcc tttttcacaa agaagtgggg atatgcccaa tctatcacat ttttgtccca
    12541 tttagaaata ttggagagca gtgtatttaa tggtttttga ctttgagaat tgtatggtat
    12601 tgaaaagtgg cgattactcc gctgaactgg attcttttgc ctggatatct caacaaagcc
    12661 agtatcgctt catagtgtaa gtcaccggtg gattataggt acgagaaaag atatataata
    12721 cagacttgtg agtaatgagt ttatcaatgg aagatctata accttgatta aaagcgatta
    12781 tcatcttttc tgctctctta gcaacaaact ctttacatta aattccgata aaagatctca
    12841 cgtatattga caaagccgag ttaattaggt agaattatat caagaagaac gagaagacag
    12901 taagaaccct tgctacccca agaggaaaac ggtcactgaa aagacaggag acagcgcaaa
    12961 gaaccctagt caccaacgtg caaggtcagt catcaccccc cagattctcg atggatggac
    13021 aggcacgttg gaacccccct agaaaatccc gattattgag ccgccgcaag gcggcttttt
    13081 ttcttgcggg aggccaatga aaatgacgaa cacgcacttc cgttgatatg tttatctctg
    13141 agagtctttc aagctttcta caaagggcag gtcggcgggg gagaaatcgt aaacgtcaag
    13201 ttcatgcaca cagatccagc gaacttcaga atgatccttc agttcgaact cctcagagat
    13261 aaattcaaca ctgaaggctt ccagcctgat ctttccaaac gggtactcgt aaattgaggt
    13321 gccgaagtga ccctgaatgt taatctcgac cccgagttct tcttttattt cgcgtttcaa
    13381 acattgttcc agaccctcgc cagtttcagt tttaccaccg ggaaactccc attttccggg
    13441 taattttccc ttatcgagcc tcctggctat cagaaccata ccgtttctct gcgcgattgc
    13501 ggccgtcact acaatcaaaa gatcactccc cattcaagat tcttttgaaa tagattctct
    13561 cgtgaatcca accagtggca ggcgatgata aagcccctag acgaagagac cggaagaaac
    13621 cggttcttca aagatctgcc ggttctccca taattctttt cagcgtgttc tcatcgagta
    13681 tctttatctt tttcccgtcc tggctgatga ttcctccgga ggtgaattcc ccgaaaaccc
    13741 ttgacaggga gggcctagca acactgaagg cgtctgccag ctcttctctt gttatcttaa
    13801 gttcgaactc cgggccgttc tccgaggctt tcttcaaaag aaaatgggcc agtttttggc
    13861 tgatcgtatt cagcgatagc aaccatactt tctcggccag agtggtaagc ctgtcgccca
    13921 tatcaccgag gagattgacc aggaatctct cgttgtttcc gcatatggag atgattccag
    13981 ttctatccat gtagagtatc gtgacgtgat cgatggcaac tatatcgacc ggcaacctac
    14041 tgtagctggc gaagatgaaa cctgatgcca gaatcgaggg agccttgatc ctctcaatct
    14101 gaac
//
LOCUS       DAOOLY010000027        14045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4696_length_14045_cov_4.868192, whole genome shotgun sequence.
ACCESSION   DAOOLY010000027 DAOOLY010000000
VERSION     DAOOLY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4696_length_14045_cov_4.868192"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLD15_02540"
     CDS             <1..479
                     /locus_tag="PLD15_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPB62806.1"
                     /translation="DIDSDGIVHVSAKDLGTNREQSMVVSGRQKMSEEDIKRIVEDAR
                     KYEEQDKKKKQEIELKNQADQLAYSIEKLLKDSGDKISSEDRGKLEGLVKDLRDAINQ
                     DNIQRVKLLFDQLQKESMRVGQSVYQKTQAQTGQGPESGGEGNDGGTEYIPPEDNQ"
     gene            512..958
                     /locus_tag="PLD15_02545"
     CDS             512..958
                     /locus_tag="PLD15_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPB62807.1"
                     /translation="MLAIRRSDVFRPFEEIQKEMDRLFNEAFKGFNNQPRETSMISPE
                     VDIYEKDNSVFIEMDVPGIKKDELEIKVEEDILSIRGEKKLEREDKGRDYHRYERCCG
                     SFQRIFRLPEYVSSEEIKARYEDGVLKIELPKKEEVKKEAVQVKID"
     gene            1014..3392
                     /locus_tag="PLD15_02550"
     CDS             1014..3392
                     /locus_tag="PLD15_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPB62808.1"
                     /translation="MINYDEYTEKAKKILMDVQDILTRYRQNQLASEHILLSMLEDDD
                     NIAVDILKEKNGNTEALRRDVEEVIGRYGSSGQSTSQIFITPEARHILENARSESRRM
                     QDSKVGTEHILLSMVRETSAVASRLLQKYGINIDNIYSLILKRRNVAESEESENVNSL
                     KKYTIDLTQLAREGKLMPVIGREDEVRRVIQIIGRKTKNNPALVGEPGVGKTAIVEGL
                     SQRIVSGDVPHYLKDKRVLALDMGRLVAGTKFRGEFEERMKDIIDSVKKLSGDVILFI
                     DEIHSVVGAGSAEGSMDAANLMKPALARGELQCVGATTLDEYRKYIEKDRALERRFQP
                     VLVDEPTTEEAYEIMKGLKETYEKHHEIKITDEALKTAVDLSVKYIPDRYLPDKAIDL
                     IDEAASFVRLKAGYMPDDLRSMDRELKELDAQITALAEKGDYKAAAESKTRAEQLKKE
                     YLAEKTRWETSESISSMVTPDIVASIVEQWTGIPAGKMLQSERDRLKNLESLIHERFM
                     DQNEAVDVVAQTIRRARAGLKDPDKPWGSFLFMGPTGVGKTELAKTLAFLLFGSEEAM
                     VRLDMSEYMEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLLDEIEKAHPDVH
                     NVLLQTMDDGRLTDGKGKTVSFANTIIIMTSNIASEELADIQENPGAREIAGEKLRRT
                     FRPEFLNRLDAIVLFEPLDPSAMKGIVRLQLSEIEERLREQNICIDVTDGAVEYLAEN
                     GYDKLYGARPVRRLIERTIEGPVADMIIDGKLQEGVRVSVDTDELGIKLDIL"
     gene            3474..4427
                     /locus_tag="PLD15_02555"
     CDS             3474..4427
                     /locus_tag="PLD15_02555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HPB62809.1"
                     /translation="MDKIFYTYRIPDEGMLLLKNYEISGNNEDRFLSKEEIIKGARDA
                     SALISLLSDRIDSEVISSLPKLKVIANYAVGYNNIDIEEARKRGIRVTNTPGALTDAT
                     ADLTMALLLATSRRIVEGDRLVRERKFEGWKPGLLKGPALKGKILGIIGMGRIGKTVA
                     SRAHAFGMNIVYHNRKPLLQSEEEELCVKHVSLEELLKSSDFISLHVPLMGETYHLLN
                     EKRLSLIKPESIIINTSRGAVIDEKALIKALKDGKLAGAGLDVYEEEPFVPQELIDLP
                     NVVLLPHLGSATNEARREMAIMVGRNVAAVLEGKEPPNLVI"
     gene            complement(4442..4876)
                     /locus_tag="PLD15_02560"
     CDS             complement(4442..4876)
                     /locus_tag="PLD15_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62810.1"
                     /translation="MERVFFTVLLTIFVVALAASLTPYSEATFSSYLQDGAYVYGGSV
                     MLGWDVTVRGSSLVLNNIDIWFSSRNSGGSIGIKSAPYIEVVSYNVYLGTTPTPQLYT
                     STASNRVMIDRLSPNMTYYWQIVAVDRNGKRYSGPIWYFVTR"
     gene            4949..5074
                     /locus_tag="PLD15_02565"
     CDS             4949..5074
                     /locus_tag="PLD15_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HPB62811.1"
                     /translation="MKVKKVGEVYRCEICGNVVEVKEVGGGELVCCGEPMKLVKE"
     gene            complement(5186..5614)
                     /locus_tag="PLD15_02570"
     CDS             complement(5186..5614)
                     /locus_tag="PLD15_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62812.1"
                     /translation="MKRSLFALSVAGLLVVFMLSGCFIITPVVGVSNLINDFEKYWEQ
                     ESAVGLGALYTEPAFVGGTSYTRNQIGSLYGLVFLIKDVSYFEFLSDKNISFKTGLNE
                     ADVTFFARIGYMDGTFVENNYNWTVVKIGNKWYITQSNSL"
     gene            complement(5751..6530)
                     /locus_tag="PLD15_02575"
     CDS             complement(5751..6530)
                     /locus_tag="PLD15_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPB62813.1"
                     /translation="MKKVLLVLVIVMAIIVCGATKVKIGVTPIPFVDILEFIRPQLEE
                     AGIELEIVVFSDYVLPNLALSSKELDANFFQHAQYLKSFTAQRGIKGLVSAVNVLIAP
                     MGFYLKKPLEDLKKGDKIALMNDPTNETRALLLLHNNGLITLKDPSKADLTVRDIQDN
                     PKGLVLVEMEAGFVPRVFKEDDTVVGAVLNANYALSIGLNSQKDATFVEDSNSPYANF
                     IVVREEDLNSQWLATLKQIITRDEVRQFIREKFEGSIVPTF"
     gene            6909..7736
                     /locus_tag="PLD15_02580"
     CDS             6909..7736
                     /locus_tag="PLD15_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPB62814.1"
                     /translation="MDFLLSSNVLIDFPLRDALEIFAQKKVDGVEIWVEHLWKEKSSL
                     EDLRGFMDSLGLKRTLHAPTRDVNITSSNPGIKAESIRQTLEALEIAYRLGIDVVTVH
                     PGHMSSSKDRPGDFFEEQTQALFRIGKKAKELGIKVGVEVMERKPGELVVAPEDLNVL
                     LDIVNLDSLGTTFDIAHAATHYKDSVEPAQLNGSIVDYMKRLRKIYNVHVSNSSLEKV
                     HLPLSRGAYDFLPVMKELSEIYEGTVSIEGFVPGDGMRVLEENMNVIHRWKESLSNL"
     gene            7746..9278
                     /locus_tag="PLD15_02585"
     CDS             7746..9278
                     /locus_tag="PLD15_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB62815.1"
                     /translation="MNLREAILSQYDKGLNDYWIPPLVACDGESRPGRIQDGDTVIFC
                     CRRGEREVQLMESFVEKRFTAFPVRDFSRLRFVPLVEYHEKFSKIEPIVPPVRPERTL
                     GEVLSGEGKKQLAVTESEKESHVTFFFNGRHSRLYPGQVAEIIPSWKDFKNHPEMKSA
                     EIAKAVVKAMDRYDFIIINFPAGDVIGHLLEMDLKVKAAEEIDKALGAIYSQATSLGR
                     TLIITADHGLMERGFSDEGTPSVSHTTAPVPFIVVNPRLGKGDIIRDGCSLVDVAPTV
                     LSLMGMRKPEEMTGRSLLKRVPVSSGVVLVILDGWGEGSDDPSINPLKAASTPNLDFL
                     KREFPFTRLKASEEYVGLPHGRSGNSETGHLTIGAGRLIEQDELRLMRVIERRFEDNP
                     VLERVLNGVKGGASLHLIGMLSEASSHGNINEILSISKVAESKNIERIFSHLILDGRS
                     APPRSAVDLLEKYREELGKTEVVTAVGRGYALDRSKDYTGKTKPVYDALVEGKGECYY
                     GL"
     gene            9295..10155
                     /locus_tag="PLD15_02590"
     CDS             9295..10155
                     /locus_tag="PLD15_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB62816.1"
                     /translation="MERDVTILDRVREVYPTLTKSQKLIADSILENWENIAFVSPREL
                     GARLGVSETTVIRFARSLGYESFTDLRQHSQDLIKERLTPAEKMSATVRKIKSGETSF
                     ERLLTAEVENLKEGISKVSPDEFYRAVDLIEGAQRVFVTGQGVSESLCKFLQFRFRRM
                     QIDTRVVTGGGKSFYETLLLMNDRDVLLGIGFFRCSQDILRAFDYAKKRSIPTIALTH
                     STVSELALRSDVTLVANRGPVSLLNSLVVPMAILNALVLYLAERDEEKLKALKKLDEI
                     PNIFGLNGKE"
     gene            10185..11165
                     /locus_tag="PLD15_02595"
     CDS             10185..11165
                     /locus_tag="PLD15_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62817.1"
                     /translation="MRKYAIVILLLICSAVFFGAEKIMLYTSVPTEIMTAIADAFMAA
                     NPGIELEVFRSGTGTIQTKLAAEAQAGNIQADVLWVAEFSYYETLKKEGLLAQIFPEE
                     AASLPENFKDPEGYYYAGRLINMVIAYNTKALTPETAPKSWKELTDEKWYDNYVVPNP
                     EYSGAAVAAVGALAKKYGWKYFEDLRENEAVVVRGNSDVAQKVAAGEFPIGMTLDYIA
                     RDLNVKGSPIDIIYPTDGTIAIPSPIAILKSSQKMEAAMKFVNYILSIDGQKAQVELG
                     SLVPVRSDVNPPANTPPAGEILGQAMEIDWKYIEEGLAEINTNFSDIMLF"
     gene            11228..12901
                     /locus_tag="PLD15_02600"
     CDS             11228..12901
                     /locus_tag="PLD15_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB62818.1"
                     /translation="MQRKIKADPFILGGTFVVIAILVVFPLTLLFLNSFKFDGAYTLQ
                     NYATVFQAKRNYTALFNSLKLGLFTSLFSILLGAPLAWLVTRTNLPFAKLFRTLFILP
                     YMIPPFIGAIAWSQLLNRRNGYINRLIQSIFGIEGYVFNVNTMPGLIWVMTLYLYPFV
                     FITVAGALERMDPTLEEAARTCGSKTMKIMRDITLPLVAPSIAGGALLVFASSIANFG
                     IPALVGMQGRVFVLTTMIYSYMHSGGFNGIATASALSIMLMAISVGALLINNFYLKKK
                     QYTLISGKSMRPKTLDLGAWKIPCLIVAIAIVFVAVIMPFFSIGLTSLLDAWGADITW
                     ENLTFRNYRYILFEYKLTKDALKNSFLLAISAATICMVLGSLIAYISVKTKIRGRKAL
                     ETIAQIPYTIPGTVVAIAMILAWSGNYGVNLYNTFWILLVAYVAHYVAFAVRTTSASL
                     EQIHTSLEEAARISGGSWLTSLKDIVVPLIRPGLVAGWFLIFMPTLRELTMSILLWGP
                     RTVTIGVAVFEMQEAGYVQISAAFATLLLLIVIAGNLLVKWLTRGKVGI"
     gene            12917..13987
                     /locus_tag="PLD15_02605"
     CDS             12917..13987
                     /locus_tag="PLD15_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62819.1"
                     /translation="MSKVVLKGLSKSFGEVNVVNNIDLEINDREFLSFLGPSGCGKTT
                     TLRMIAGFDMPTTGEIFIGERLVSSVDKKIFVPPEERNVGMVFQNYAVWPHMDVFKNV
                     AYPLKIKKTGADEMKERVLKVLSMVKLTGMEKRYPHQLSGGQQQRVALARALVMEPDV
                     MLLDEPLSNLDAKLREEMRFEIKDIQRRIGVTIIYVTHDQAEAMAMSDRILLLHNARV
                     QQLGTPRDLYERPENQFVADFIGLINFLKIDVADRDARTMATFTSSPETPPFEVNLEN
                     TGKSSEGGFVFAVRPENVKLVPPDSSSIRGKVTKKVYLGNILDYRVALGKEEIRVEAS
                     SGEEFQPGQTVGLRFESYVLFN"
BASE COUNT         3739 a         3532 c         3836 g         2938 t
ORIGIN      
        1 tcgatataga cagcgacgga atagttcacg tatcggcaaa ggatcttgga accaacagag
       61 aacagtcgat ggtggttagc ggaagacaga agatgtccga agaagatata aagagaatcg
      121 tcgaagacgc caggaagtac gaagaacagg acaagaagaa gaaacaggaa atcgaactga
      181 agaaccaggc cgaccaactc gcctacagca tagaaaagct ccttaaggat agcggcgaca
      241 agatctcttc cgaggacagg ggaaagctgg aaggacttgt caaggatctg agagacgcga
      301 taaaccagga caacatacag agagtgaagc tgctcttcga tcagctccag aaggaaagca
      361 tgagagtcgg acagagcgtt taccagaaga ctcaggcaca gaccggtcag ggacctgaga
      421 gtggcggcga aggtaacgac ggcggcaccg agtacatacc gccggaagac aaccagtgaa
      481 caaaataata taaacaacgg aggtgtttaa aatgttggct atcagaagaa gtgatgtttt
      541 cagaccgttc gaagagatcc agaaggaaat ggacagactt ttcaacgaag ccttcaaagg
      601 gttcaacaac cagcccagag agacctctat gatcagtccg gaagtcgata tctacgaaaa
      661 ggacaactcg gttttcatcg aaatggatgt acccgggata aagaaggacg aactggagat
      721 aaaagtagag gaagatatac tctcgataag gggcgagaaa aagctcgaaa gagaggacaa
      781 gggaagggac taccaccgct acgagcgctg ctgcgggtcc ttccagagga tcttcagact
      841 acccgagtat gttagctcgg aagagataaa agccaggtac gaagacggtg ttctgaagat
      901 cgagcttcca aagaaagagg aagtcaagaa agaagccgtt caggtcaaga tcgactgaag
      961 aggctgaagg atggtggggg cgctcgaagg cgcccctttt ttggaggtgt agaatgataa
     1021 actacgacga atacaccgaa aaggcaaaaa agatactgat ggatgtacag gatatactca
     1081 cgcgttacag acagaatcag ctggcctccg aacacatact cctgtcgatg ctcgaagatg
     1141 acgacaatat agccgtggat attctcaagg agaaaaacgg taatacagag gctttgagaa
     1201 gggacgtcga agaggtaatt ggccgctacg gcagctccgg tcaatcgacc agccagatct
     1261 tcataactcc cgaggcccga cacatactgg aaaacgccag gagcgagtcg agaaggatgc
     1321 aggattccaa ggtcggaacg gagcacatac tcctgtcgat ggttagagag acttcggcgg
     1381 tcgccagcag actccttcag aagtacggca ttaatataga caatatctac tctctcattc
     1441 tgaaaagaag aaacgtggcc gaatcggaag agagcgaaaa cgtgaactcc ctgaagaaat
     1501 acactataga cctgacacaa ctggccagag aaggcaaact gatgcccgtg atcggcaggg
     1561 aggacgaggt tcgcagggtt attcagatca taggcaggaa aaccaagaac aacccggccc
     1621 tggtcggcga gccgggtgtg gggaagacgg cgatcgtgga gggtctttcc cagagaatag
     1681 tgagcggaga cgttccgcat tacctgaaag acaagagagt cttagccctc gatatgggaa
     1741 gactggtagc cggaacgaag ttccgtggag agttcgaaga gaggatgaag gatataatcg
     1801 actcggtgaa gaagctctcc ggcgacgtga tcctcttcat agacgaaatc cacagcgtag
     1861 tcggcgccgg ctcggccgaa ggctcgatgg acgcggctaa cctcatgaaa ccggccctgg
     1921 cgaggggcga actccagtgc gtgggtgcca ccactctcga cgaatacagg aaatacatag
     1981 agaaagacag agcgctcgag agaagattcc agccggtgct ggtcgatgag ccgaccacag
     2041 aagaggctta cgaaataatg aaaggcctga aagagaccta cgagaaacac cacgagataa
     2101 agataaccga tgaagcgctg aagacggccg tcgacctgag cgtgaaatat atccccgaca
     2161 gatacctccc cgacaaggcc atcgacctca tagacgaggc cgcgtccttc gtcaggctta
     2221 aggccggcta tatgccggac gacctgaggt ccatggacag ggaactgaag gaactggacg
     2281 cacagatcac cgcgctggcc gagaaaggcg attacaaagc ggccgccgaa tcgaaaacca
     2341 gagccgagca gctcaagaaa gagtatctgg ccgaaaaaac cagatgggaa acgagcgaat
     2401 ctatcagcag tatggtcaca ccggatatag tggcctctat agtagagcag tggacgggaa
     2461 taccggccgg aaagatgctc cagtcggaac gcgacaggct gaagaacctg gaatcgctga
     2521 tacacgagag attcatggat caaaacgaag ccgtcgatgt ggtagcccag acgatacgcc
     2581 gcgcaagggc cggcttgaaa gatcccgata aaccatgggg ctcctttctc ttcatgggac
     2641 cgaccggggt gggaaagacc gagctggcca aaacgctagc cttccttctc ttcggaagcg
     2701 aggaggctat ggtgaggctg gatatgtcgg aatacatgga gaaacacaca gtatccagac
     2761 tgattggcgc ccctccgggc tatgtgggtt atgacgaggg cggacagttg accgaggccg
     2821 tcagaagaag accttactcg gtggtcctgc tggacgagat cgaaaaggcc catcccgacg
     2881 ttcacaacgt acttctgcaa accatggacg atggaaggtt gaccgacggc aaggggaaga
     2941 cagtctcctt cgccaacacg atcataataa tgaccagcaa catcgcttcg gaagagctgg
     3001 cggatataca ggagaatccc ggggccagag aaatagccgg tgaaaaactt cgcagaacgt
     3061 tcagaccgga gttccttaac agactggatg ccatcgttct cttcgaaccg ctcgaccctt
     3121 cggcgatgaa gggaatagtc aggttacagc tctcggagat agaagagcga ttaagagagc
     3181 agaacatatg tatagacgtt accgacgggg ccgtcgagta cctggctgag aatggatacg
     3241 ataaactgta cggtgcgagg ccggtgagac gtcttataga aagaaccata gaaggacccg
     3301 tggccgatat gataatagac ggtaaactcc aggaaggagt cagggtgtcg gtcgatactg
     3361 atgagcttgg aataaagctg gatattttgt aacctttcaa gaccggagag gtctctctct
     3421 ccggtctttt tcttcaaata caagatgata tcattatcac aggaggtgcc tgaatggaca
     3481 aaatattcta cacttacaga atccccgatg aaggtatgct tcttctcaag aactacgaaa
     3541 tctccggaaa caacgaggat cgttttctaa gcaaagagga gataatcaaa ggggcccgcg
     3601 acgcctctgc gctcatttcg ctgctctcgg acaggataga ttccgaagtt atttcgtcgc
     3661 tgccgaaact gaaagtgata gccaactacg cggttgggta caacaacata gacatagaag
     3721 aggccagaaa gagaggaata agagtgacca acactccggg cgccctgacc gatgcgaccg
     3781 ccgatctcac catggctctg ctgctggcaa ccagcaggag gatagtcgag ggcgacagat
     3841 tagtgagaga gcgcaaattc gaaggctgga agcccggcct gctcaagggc cccgccctca
     3901 agggaaagat cctgggaata attgggatgg gcaggatagg aaagaccgtg gcgagcagag
     3961 cgcatgcctt cggcatgaac atcgtctatc acaacagaaa acctctcctg caatcggagg
     4021 aggaagaact ctgcgttaaa cacgttagtc tggaagagtt actcaagagc tccgacttca
     4081 tctctttaca tgtaccgctc atgggtgaga cataccatct gctcaacgaa aagagactct
     4141 ctctcatcaa gccggagtcg ataatcatca acacctctcg cggagccgtc atagatgaaa
     4201 aagcactgat caaggctctc aaggacggaa aattagccgg cgccgggctc gacgtctatg
     4261 aggaggagcc gttcgtgccg caggagctga tcgatcttcc aaacgtggtt ctcctgcctc
     4321 acctcggatc ggccaccaac gaggccagaa gggaaatggc cataatggtc ggacgaaacg
     4381 tagcggccgt tctcgaaggc aaagagccac ccaatctcgt tatttgagac gcttttcccg
     4441 gttaccttgt cacgaagtac cagatgggtc cggaataacg tttgccgttt ctatccaccg
     4501 ccactatctg ccagtaatag gtcatattcg gagacagcct gtctatcatg accctgttcg
     4561 atgccgtact ggtgtaaagt tgaggggtgg gggtggttcc cagataaacg ttatagctca
     4621 cgacctctat gtacggcgcg gatttgattc ctatactacc gccggagttc ctgctcgaga
     4681 accagatatc tatgttgttg agaaccagtg agctgccacg gacagtaacg tcccagccga
     4741 gcattacact ccctccataa acataggctc cgtcctggag atagctggaa aaagtggctt
     4801 cactataagg tgtcagcgat gcggccagcg ccaccacaaa gattgtaagg agcacggtga
     4861 aaaaaactcg ttccattctt acacctccat ttatcgtttt gactgataga agcaacgtat
     4921 ggttttacta ccgaaaggag ttgagaaggt gaaagttaaa aaagttggag aggtttatcg
     4981 ctgcgagatc tgcggaaacg tcgtggaagt gaaagaagtc ggcggcggcg agctggtctg
     5041 ctgcggagag ccgatgaaac tggtcaagga atgaagaaaa gccccggact ccggggcttt
     5101 tttgttgaac agatatgtcg atgcaacagc tcgaagtcgg ccgagaacgg ccgcaatttt
     5161 catttgtacg gtcccccgat tgcgactata gggagttgct ttgagttatg taccatttat
     5221 tgccgatctt cacgacggtc cagttgtagt tgttttcgac gaacgtgccg tccatataac
     5281 cgattctcgc aaaaaaagtc acgtcggcct cattcaaacc ggttttgaaa gagatattct
     5341 tatctgaaag gaattcgaag taactcacat ccttaatgag aaataccagg ccgtacagac
     5401 tccctatctg gtttctggtg taactggttc cgcctacaaa cgccggctcg gtgtagagag
     5461 ctcccagccc gacagcgctc tcctgttccc agtacttctc gaaatcgttg atcagattgc
     5521 tgacgccgac caccggcgtg atgatgaaac aaccgctcag cataaaaacc accagcagac
     5581 cggcaacaga tagcgcaaat agtgatctct tcacctaaac ccctcccctt gaaagttttg
     5641 aagacgactc aattatacgc cttgctcctt cctttgaaac cctccagcct gtattgaaca
     5701 ttaaaaagcg gccattgaag gccgctttgc acaatctaga tccctgtgtt ctagaaagtg
     5761 ggaactatgg atccttcgaa cttctcccgg atgaactgcc tcacctcatc gcgcgtgatt
     5821 atctgcttta gagtggctag ccactggctg ttcaggtctt cttcgcgaac gactatgaaa
     5881 ttggcgtacg gcgaattgct gtcctcaacg aaagtggcat ccttctgtga attcaatccg
     5941 atcgacaggg cgtaattggc gttcagaacc gcccccacaa ctgtgtcgtc ttctttgaac
     6001 actctcggca caaaacctgc ctccatctcc accagtacca gcccctttgg gttgtcctgt
     6061 atatccctca ccgtcaaatc ggccttagac ggatccttca acgttataag gccgttgttg
     6121 tgaagcagga gcagcgccct cgtctcgttg gtcgggtcgt tcatcagggc tattttatcg
     6181 cccttcttca gatcttcgag cggctttttc aagtaaaagc ccatcggagc tatgagaaca
     6241 ttgacggccg aaaccagtcc tttgatccct ctctgtgcgg tgaaggattt caaatactgg
     6301 gcgtgttgaa agaagttggc atccagttcc ttcgaagaaa gcgcaagatt tggaagaaca
     6361 tagtcgctga aaacgactat ctccagctca ataccggcct cttccagctg aggtctgatg
     6421 aactcgagga tatccacgaa tgggatcggc gtgactccga tcttcacctt cgtagccccg
     6481 caaacgatga ttgccataac gattaccaga accagtaaaa cctttttcat agaacacctc
     6541 ctgaagaaaa taaaaacggg atgtctcgcg acaccccgta tttctcacag cgacagaaac
     6601 cctgtcagaa taacggggct tctctgcaca tcaccatgct aatagaaaat atgaacccac
     6661 gaaaaatcga caaaataaat ccctccgagt tattggaagg attatacaaa aaaagtggtt
     6721 tcgaacaaat caaatgattg tgaattgtgg gacgcacgat cctgtgctgg tggattgcct
     6781 tcgatttttt ctgtgcgcgt ttcatttttc catgtctctt gtgatattct attgctgaag
     6841 tgatagcttc attgtcacca acatatttgt tagttttgca gcgatagctt caagatggag
     6901 atgagacgat ggattttctt ttaagttcca acgtactgat agattttcct ttgagggacg
     6961 cgctcgagat cttcgcgcag aagaaggtcg atggtgttga gatatgggtc gagcatctgt
     7021 ggaaggagaa atcctcactg gaagatctgc gcggcttcat ggattctctg gggctgaagc
     7081 gcacgcttca cgctcccacc agagacgtta acatcacctc ctccaacccc ggtataaagg
     7141 ccgagtcgat caggcagacg ctcgaagctc tcgagatagc ttaccggctc ggcatagacg
     7201 ttgtaacggt tcatccggga catatgtcct cttccaaaga caggccggga gattttttcg
     7261 aggagcagac gcaggctctt ttcaggatcg gcaagaaggc caaagagctg ggaattaaag
     7321 tcggggtgga agttatggaa aggaaaccgg gcgaactggt ggtcgctcca gaggatctta
     7381 acgtccttct cgacatcgtc aatctcgact ctctgggaac gactttcgat atagcccacg
     7441 ccgccaccca ctacaaggac tccgtcgagc ccgctcagct gaacgggagt atcgtcgatt
     7501 atatgaagag actcagaaag atctacaacg tacacgtgag caactccagt ctggagaaag
     7561 ttcacctccc cctctccagg ggagcttacg attttctgcc tgttatgaag gaactctcgg
     7621 aaatctatga gggcaccgta tcgatcgaag gcttcgtacc tggagatgga atgagggtgc
     7681 tggaagagaa catgaacgtt atccaccggt ggaaagagtc gctttccaat ttatgaggtg
     7741 attttgtgaa tctccgcgaa gcgatactca gtcaatacga taaaggtcta aacgattact
     7801 ggatcccgcc gctcgtcgcg tgtgacggag agagcaggcc gggaaggatt caggatggcg
     7861 atacggttat cttctgctgt cgaaggggag agagagaggt ccagctcatg gaaagtttcg
     7921 tggaaaagcg tttcacagcc tttcctgtac gcgacttttc gaggctccgt ttcgtgcctc
     7981 tggtagaata ccacgaaaag ttctcgaaga tagagccgat cgttccgccg gtcagaccgg
     8041 aaagaacgct cggcgaagtt ctcagcgggg aaggcaagaa gcagctcgcg gtgacggaat
     8101 ctgaaaagga gtctcacgtg accttcttct tcaacggccg gcacagtcgt ctctaccccg
     8161 gtcaggtagc cgagatcata ccgagctgga aggacttcaa gaaccacccg gagatgaaga
     8221 gcgccgagat cgcaaaggcc gtggtgaagg cgatggacag gtacgatttc atcataatca
     8281 acttcccggc cggcgacgtg ataggccatc tcctggaaat ggacttgaag gtgaaggccg
     8341 cagaagagat cgacaaagcg ctgggcgcga tctacagcca ggctacatcc ctcgggcgca
     8401 cgctgataat cacagccgat cacggtctca tggaaagagg cttctccgat gaaggcaccc
     8461 cttcggtctc ccacacgacg gcgccggttc ccttcatagt ggtcaacccc cggctcggga
     8521 agggcgatat aatcagagat ggctgctctc tggtcgatgt ggctcctacg gtgctctcgc
     8581 tgatgggcat gcgcaaaccc gaggagatga caggccgctc acttctcaag agagttcccg
     8641 tctcgagcgg cgtggtcctg gtcatactgg acggctgggg agagggcagc gacgatccct
     8701 ccatcaatcc gctgaaggcc gcctccactc caaacctaga tttcctgaag agggagtttc
     8761 ccttcacaag gcttaaggcc agcgaggaat acgtgggact tccacacggg aggagcggca
     8821 actccgagac cggccatctc accatagggg caggcaggtt gatcgagcag gacgaactca
     8881 ggctgatgag agtcatagaa cgccgcttcg aagacaaccc cgttctcgag agggtattga
     8941 acggcgttaa aggtggagcc tcccttcatc tgataggcat gctttctgag gccagctcgc
     9001 acgggaatat aaacgagatt ctgtcgatct cgaaggtggc cgagtcgaag aatatagaga
     9061 gaatcttttc gcacctgata ctcgacggtc gcagcgcgcc gccaagaagc gccgtcgacc
     9121 tgctcgagaa gtaccgcgaa gaactcggaa agaccgaagt cgtaacagct gttggaaggg
     9181 gttacgccct ggacagaagc aaagattaca cagggaaaac aaaaccggtg tatgatgcac
     9241 tggtagaggg taaaggcgag tgttattacg gactataaac gggaggaagg gctcttggaa
     9301 agagatgtga ctattctcga cagggtcagg gaggtctatc ccaccctcac taagtcccag
     9361 aagctcatcg ccgacagcat cctcgagaac tgggaaaaca tcgccttcgt ctctcccaga
     9421 gagctggggg cgaggctcgg agtgagcgaa actacggtga taaggttcgc cagatcgctt
     9481 ggctacgaaa gcttcaccga tctcaggcag cacagccagg atctgataaa ggagaggctg
     9541 actccggccg agaagatgtc ggcgacggtg aggaagataa aatccggcga gacatctttc
     9601 gaacgattgc tgacggccga ggtagaaaac ctgaaagagg gcatctccaa ggtttcgccg
     9661 gatgaattct atcgtgccgt cgaccttatc gaaggcgctc agagggtctt cgtgacaggg
     9721 cagggcgtgt cggaatcgct ttgcaagttc ctccaattca ggttcagacg aatgcagatc
     9781 gacaccaggg tcgtcaccgg cggtgggaaa agtttctacg agacgcttct tctcatgaac
     9841 gatagggacg ttctcttggg tataggcttc tttcgttgct cacaggacat actcagggcc
     9901 ttcgactacg cgaagaaacg gtcgatccct acgatcgcac tcacgcacag taccgtttcc
     9961 gagctggctt tgaggtcgga tgtgactctg gtagccaaca gagggccggt ctccttattg
    10021 aactcgctgg tcgtcccgat ggcgatactc aacgccctgg tcctctatct ggcagaaagg
    10081 gacgaagaga aactgaaagc gctgaaaaaa ctggatgaaa ttccaaacat cttcgggctt
    10141 aacgggaagg aatagtttcc cgggccggtg caggaggttg tctaatgaga aagtacgcaa
    10201 ttgtaattct gctactgatc tgtagcgcag tgtttttcgg ggccgagaag ataatgcttt
    10261 acacctcggt tccaacggag atcatgacgg ctatcgccga tgccttcatg gctgcaaacc
    10321 ccggaatcga actggaggtc ttcaggtccg gtaccggaac catccagacc aagctcgccg
    10381 ccgaagcgca ggccggcaac atacaggccg acgttctctg ggtcgccgag ttctcttact
    10441 atgaaactct gaagaaagag gggctcctgg cgcagatatt ccccgaggag gccgcgtctc
    10501 tgccggagaa cttcaaggat ccggaaggct attactacgc cggaaggcta atcaatatgg
    10561 ttatagccta caacaccaag gcgctcacac cggagacggc tcccaaatcc tggaaggaac
    10621 tgacggatga aaagtggtac gacaactacg tcgtgcccaa cccagagtac tcgggcgctg
    10681 ccgttgccgc agtcggcgcg ctggccaaga aatacggctg gaaatacttc gaagatctga
    10741 gagagaacga ggccgtagtt gtcaggggaa acagcgatgt cgcgcagaag gttgcggccg
    10801 gggagttccc gataggcatg actctcgact acatagcccg cgacctcaac gtcaaaggca
    10861 gcccgatcga catcatctac cccaccgacg gaacgatagc catcccgagc cccatcgcga
    10921 ttttgaagtc gtcgcagaag atggaggccg ccatgaaatt cgtcaactac atcctctcta
    10981 tcgacggcca gaaggcacag gtcgaacttg gttcgctggt tccggtccgg agcgacgtca
    11041 atcccccggc caacacgccg ccggccggcg agatactcgg tcaggcgatg gaaatcgact
    11101 ggaagtatat cgaagaagga ctcgccgaga tcaacacgaa cttctccgat atcatgctgt
    11161 tctgatggtt ttcggcccgg ccgtgaaagg ccgggtctta ctttcatcct gaggagtggt
    11221 acccttgttg cagagaaaga tcaaggccga tcccttcatt ctgggcggga ctttcgtggt
    11281 gatagccatt ctcgtggttt ttcccctgac gctgctgttt ttgaacagct tcaaattcga
    11341 cggggcctac acgcttcaaa actacgctac ggtctttcag gcgaagagaa attatacggc
    11401 tctcttcaac agtctcaagc tcggtctctt cacatcgctt ttctccattc ttctgggggc
    11461 gccgctggcc tggctcgtta ccagaaccaa ccttcctttc gcgaagctct tcagaacact
    11521 gttcatatta ccctacatga tcccgccgtt tatcggcgcg atagcctggt ctcagttgct
    11581 caacaggcga aacggttata taaacaggct tatccagtcg atcttcggca tagaaggata
    11641 cgtcttcaac gtcaacacca tgccgggcct catatgggtg atgacgctct acctgtaccc
    11701 cttcgtgttc ataaccgtgg ccggggcgct cgagaggatg gacccgacgc tcgaggaggc
    11761 agcaagaacg tgcggatcga agacgatgaa gatcatgcgc gatatcaccc ttcctcttgt
    11821 cgctccgagc atagcgggag gagcgctcct ggttttcgcc tcgagcatag ccaacttcgg
    11881 cattccagcg ctcgtgggaa tgcagggaag ggtcttcgtt ctgacgacga tgatctactc
    11941 ttacatgcac agcggtggtt tcaacggaat agccacagct tcggccctgt cgatcatgtt
    12001 gatggcaatc tccgtggggg ctctactcat aaacaacttc tacctcaaga agaaacagta
    12061 caccctgata tctggaaaga gcatgaggcc caagacgcta gatctgggtg cctggaagat
    12121 accctgcctg atcgtcgcca tcgcgatagt ctttgtagcc gtcatcatgc ccttcttctc
    12181 cataggcctg acatcgctgc tggatgcctg gggcgccgac ataacttggg agaatctcac
    12241 cttcaggaac tacaggtaca tcctctttga atacaagctc accaaagacg cgctgaagaa
    12301 cagcttcctg ctggctattt cggccgcgac gatctgtatg gtactgggct ctctgattgc
    12361 ctatataagc gttaaaacga aaataagagg ccgcaaggcc cttgagacca tagcgcagat
    12421 accctacacg ataccgggta cggtggtggc gatagcgatg atactggctt ggtcgggcaa
    12481 ttacggggtg aacctctaca acaccttctg gatactcctc gtcgcttatg tggcacacta
    12541 cgtggccttc gccgtgagga cgacgtcggc ctctctggaa cagatacaca cctcgctgga
    12601 agaggcggcc aggatctctg gcggaagctg gctcacttct ctgaaagata tagtcgtgcc
    12661 gttgataagg cccggcctcg tggctggctg gtttttgata ttcatgccga ccctgaggga
    12721 gttgacgatg tctatactcc tctggggacc caggacggtc acaataggcg tcgcggtgtt
    12781 cgaaatgcag gaggccggat acgtgcagat atcggccgcc tttgccaccc tgcttctgtt
    12841 gatcgtcata gccggaaacc tcctggtgaa gtggctcacc aggggaaaag ttggaatata
    12901 gaggggagcg agtcgaatgt caaaagttgt tctcaaggga ctttcgaagt cattcggtga
    12961 agtgaatgtc gtaaataaca tagacctgga aatcaacgat cgtgagtttc tctcctttct
    13021 cggcccttcg ggctgtggaa agaccaccac gttgagaatg atcgccggtt tcgacatgcc
    13081 cacgaccggg gagatcttca tcggcgagag actcgtctcg tcggtggata aaaagatctt
    13141 cgttcctccg gaagagagaa atgtcggcat ggtcttccag aactacgccg tctggccgca
    13201 catggacgtc ttcaagaacg tcgcctaccc gctgaagata aaaaagaccg gtgccgacga
    13261 gatgaaagag agggtgttga aggttctctc catggttaag ctgacaggaa tggagaaacg
    13321 ctatccccac caactttcgg gtggtcaaca gcagcgcgtc gcgcttgcca gggcgctggt
    13381 catggagcct gacgtcatgt tgctcgacga acctctaagc aacctcgacg cgaaactgcg
    13441 agaggagatg cgcttcgaaa taaaggacat tcagcgccga ataggcgtca cgatcatcta
    13501 cgtcactcac gaccaggcag aggctatggc tatgtccgac cggatactcc tgctccacaa
    13561 cgccagagtg cagcagctcg ggacacccag ggacctgtac gagcgtcccg aaaatcaatt
    13621 cgtcgccgac ttcataggcc tgataaactt cttgaagata gacgtcgcgg accgggacgc
    13681 tcgtacgatg gccactttca catcctctcc ggagactccg ccttttgaag tgaacctcga
    13741 aaacaccgga aagagcagcg aggggggctt cgtttttgcc gtcaggcccg agaacgtaaa
    13801 gctagtccct cccgatagca gctcaataag aggaaaagtg accaagaaag tctatcttgg
    13861 caatatactc gattaccgtg tggcgctggg aaaggaagag atccgtgtgg aggcctcctc
    13921 cggcgaggag ttccagcccg ggcagaccgt gggactgcgg tttgaaagtt acgtgctctt
    13981 caattaagat cggggatgtg aagagtttta aggattgcag ccgttagcgg ttcgatcttc
    14041 atatc
//
LOCUS       DAOOLY010000028        13993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4729_length_13993_cov_5.303487, whole genome shotgun sequence.
ACCESSION   DAOOLY010000028 DAOOLY010000000
VERSION     DAOOLY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13993
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4729_length_13993_cov_5.303487"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLD15_02610"
     CDS             <1..416
                     /locus_tag="PLD15_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HPB62820.1"
                     /translation="MLRKSIIKYKNRQLETARIIEELIELAKMMQQARKRGEELGLTD
                     DELAFYDALSENKSAVDIMGDEKLKVIAVKLLDTMRKSISIDWTLRENVKARLRTQIK
                     RILKKFGYPPDLQQQAVKTVLEQAEYICRDWTGNE"
     gene            complement(996..1586)
                     /locus_tag="PLD15_02615"
     CDS             complement(996..1586)
                     /locus_tag="PLD15_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB62821.1"
                     /translation="MNLKGTKTLENLMKAFAGESQARNRYTFYASVAKKEGYEQIAAI
                     FTETADNEKEHAKVFFKHILEGMASELPITVHVDADYPVELRTTRENLKASAAGENEE
                     WTMLYPEFADVAEKEGFKDVANSFRQIAKVENRHEARYLKLLDNIEKCKVFKREEKVL
                     WKCGNCGYIIEAKEAPDVCPACKHPKSYFELFCETY"
     gene            complement(1719..2654)
                     /locus_tag="PLD15_02620"
     CDS             complement(1719..2654)
                     /locus_tag="PLD15_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB62822.1"
                     /translation="MNKDRASIARIGSIFGVIGNAILAVMKLLTGFLTGSVAILADGM
                     DSSTDILTSLITLVSTSVSNKPPDAQHPYGHERADAVASKIVSMVIFFAGAQLAMSSI
                     EKLFSGSSSVENLPLVVTVALISAIGKYALYRYKLAIARKIDSSVFMADALNMRSDIT
                     ISLSVLLGSILVSFTDFQIIDTLVGLVVSAMVIKTAVGIFWETNFELMDGLKPGDDIY
                     NIIFEAVSRVPGVQNPHKVRVRKMGYKYLVDLDIEVDPLLTVRKAHALAKNVEKAIIE
                     GNESVYDVHVHVEPAGNVENENFGIDSAGQFEEKN"
     gene            complement(2647..4086)
                     /locus_tag="PLD15_02625"
     CDS             complement(2647..4086)
                     /locus_tag="PLD15_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB62823.1"
                     /translation="MFYELYLRSFYDGNGDGIGDLKGAEKKLDYIAELGMEGIWLLPI
                     MQSPAYHGYSVTDFFAINPIYGDLKDLRNFLYGAHKRELKVILDLPLNHTSPSHEWFL
                     KALDGEKPYRDWYLFLQSEEWLKARRHWDNQQVWSNYSGRWVYALFGPGSPDLNFESP
                     SLWQQIKEVLTFWLSVGFDGFRFDAAKHIFDFSIEKGICEYQHSRNIAFWKEMTSHCR
                     AISPDCLFVSEDWDDARIVDMYSSIFGIGFNFPLAEDLKLAIASRNAVSLVKALKTDM
                     QRFFRSRRSYESGTFLTNHDMTRLVSSMNGDRDLALLALTVLLTLPGLPFLYYGEELG
                     MEGVYDEYFNEEQLEPHLWYENGYGPGQTEWKALGKNRPHSGVSFQAQSGIAGSYFER
                     FKNILRFRRDNPWLRFTTIKSISRKKDLVRINLHGSKGNAFLYHNTGSSRATLDTAGT
                     PVVINGTLERLRGRWSLAGLSSAIEVLNE"
     gene            4222..5271
                     /locus_tag="PLD15_02630"
     CDS             4222..5271
                     /locus_tag="PLD15_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPB62824.1"
                     /translation="MLIEELLGKGEKMNASDVHITCRGNVMYRVDGELVQDVFLTDDI
                     MMRKILSELQGLTNEKFEESGKKEIDFSFGFGSSRVRGNFFYSNKLPALALRLIPKTI
                     RTLDDLGYPELFKEFCKPDKGLVLVAGPTGSGKSTTLAAMLENVNRTRHVHLITIEDP
                     VEYVFEQKNALIHQRELGPDTQSFYNGLKYALRQDPDVILVGEMRDSETMELAMTAAE
                     TGHLVFSTIHTNSAATTPERIVGVFPPHQQNQISMQLANTLMAVVYQRLLKRRDGKGR
                     VAVIEIMVVNQAIRNLIREQKFHQIESMMQAGVKFGMVTFDDALVEIFKKGIIDKDQL
                     LSYARDAGSIARRSF"
     gene            5271..6353
                     /gene="prfA"
                     /locus_tag="PLD15_02635"
     CDS             5271..6353
                     /gene="prfA"
                     /locus_tag="PLD15_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPB62825.1"
                     /translation="MNLEKIIEVFRERFKEVEAQLSRPEVASDPERLMEYGKKHAGLS
                     QIIELHERIETMKRELDDWGGMKNNSEEEELEEIERTIDELTGKIAKEELNLKALLVP
                     DLADDRNIIIEIRAGTGGEEAALFGSDLFRMYSRYAERNNWKSEVIDVNETDLGGLKE
                     IVFQIKGRSVYSKLKYESGVHRVQRVPTTESGGRIHTSTATVAVLPEVSETEVKIEPS
                     DLRIDTYRASGAGGQHVNKTDSAVRIVHIPTGIVVAVQKERSQHQNKARAMELLRAKL
                     YDMYSSKAENEVTQTRRSQIGTGERSEKIRTYNFPQNRVTDHRINFTSYRLLDIMDGD
                     IEELVTELSAADINKRLELLHKELVG"
     gene            complement(6381..7577)
                     /locus_tag="PLD15_02640"
     CDS             complement(6381..7577)
                     /locus_tag="PLD15_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB62826.1"
                     /translation="MDKLAVGMEKNIRRNYVFSFFMNLRLSGGLWMIYMAFKGMSLTQ
                     IGLLEGIFHVTSLAMEVPTGSIADLFGRKLSRTLGRCMSLASILILIVSSDFTHFLFF
                     MVFSALSYNLESGSGEALVYDSLKYLGKSEEFMRIAGKQEAVFQCSSIAAFLVGGLLG
                     TFDYHLAFWVSAAIIIFTIGYSLLFVEPPIDSDSAGSSPKRSLKGFARQIADSFGVIA
                     KTRKVAFLILFTQTILAFCTCIFFYMQNFWKGEGMNEFQIGVLLAIGSLAAGVTATKV
                     HKLQRLLREKMMLILLPLAAVISMWALALSPFKAPFFILISMFEAMLFVASSDYINRL
                     IPSQYRATIISFSSMAFSVVMIAIFPSFGKMADAFSFGIAFISLAAVGSLLYGLNMWV
                     LAKLEM"
     gene            7908..8648
                     /locus_tag="PLD15_02645"
     CDS             7908..8648
                     /locus_tag="PLD15_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB62827.1"
                     /translation="MSNIPVENRGHSSLISIIQNELKTRIINGAFKPGEKLPSEPELA
                     MELGVSRNSLREAIGLLQREGLLVKRHGVGNFVTDRYPIIRGGIERLAGITEFIRAQG
                     LKAGSKVTRFELVECPGEIGQTLDTEPGDRIFIMETIKYASEVPVALCADFIPLSIAG
                     NIDPDRIHDSIFDGLRDHYGVDIRYAECDLIPMTAGEELTGKLSVEAGTPVLLLEQIH
                     YDSLNRRVLYSKSYFPVGKFTFKLIRKR"
     gene            8667..9923
                     /locus_tag="PLD15_02650"
     CDS             8667..9923
                     /locus_tag="PLD15_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62828.1"
                     /translation="MNALRRLFVLLILFIGIFLSATTIEVLWMGWPQDQVMQLVKAFK
                     DENPTVEVDIQLVPFGQLFQTLEVRLAAGDGTPDVYIVDGPNTASYAARGYLLALDDY
                     FTDEEMDDWFSASIEEGSYDGKFYSVPYGTSSAGIFYNKAIFEEYGIPFPPEGIDERL
                     TWDEVVEIARALTKDTNNDGITDIWGLVIEQIDRPYLIFPIVQSLGARALSSDGFVSQ
                     GYINSKEFIEGTTFYWKLFNEWKISPQGVPDSAIAREYFGTGKAAMMLGNEWNIRRMS
                     QYPSVEFGLTPFPYFEGGAIVTPTGSWHVGINSKTRKLDEALKFTKFITGKEAVITWH
                     MLNGIAPARYDVYEALPEVFGNPMWQTFLYEMEHTAVARPVTPGYSQYELMLREAFNS
                     IHYGADPKKTLDDAAIRIDRELRRYK"
     gene            9966..10832
                     /locus_tag="PLD15_02655"
     CDS             9966..10832
                     /locus_tag="PLD15_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62829.1"
                     /translation="MFRNGRVAWLFILPALAFLLLFKIWPIGVSFIESLTTTSFRGTK
                     SFVGFENYSYLFRYDPVFWNSFWVTLLYSAIVNPLIVLSSLLMALLLNGSYFFTKFFR
                     TVFFLPTAISYAVVAVIWSIVLDPYYGLANSFLTSFGLKPQPFFASPDQALWGLIMLN
                     VWRSSGYWMMYFLAGLQNIPESLYEAAEIDGASTLQKTLKITLPLLTRTMSFVLVANT
                     AFNFLSFAPVYIITKGGPMGSTNLLMYESYKSAFVNADMGRASAITSILLAIILVFSF
                     FELKLTKANFEY"
     gene            10843..11685
                     /locus_tag="PLD15_02660"
     CDS             10843..11685
                     /locus_tag="PLD15_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB62830.1"
                     /translation="MKSGRLWKTTSLFIVLAVVSLFFIVPLVWIVASALRPAGSLYQY
                     ANPLTWGTFIPSKPTLENFVHIFSKLNFGRAIANSLFVSLVTILLTVFVSSMAGFSLA
                     KFNFRGKNLVFAIVLITFMVPFESIVIPLYILVKQLKIDNTYWALILPGVANGLAIFL
                     FRQFFSEVPSEIMEAARIDGASWFRIYWRILLPLSMPAIVTVIVMVFMFQWNSLFWPL
                     VATHSSRFEVVQVAIASHRSTESTSWANLFSSAIAGSLPPVILFLFLQKYFVRGISGT
                     GLKG"
     gene            11690..12505
                     /locus_tag="PLD15_02665"
     CDS             11690..12505
                     /locus_tag="PLD15_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPB62831.1"
                     /translation="MESISISKDHLKLSVDKLPDILLLPGDPARVYEIGRFLDNVEEV
                     GNNRDYLTIVGEYKGLPIAACSSGMGGPSTEIAVVELHRHGVKSIIRIGTSGGLSEKI
                     SPGDLIVLSGCIKYSGTANLFVPENFPSIADYRVLQALICACDREEAAFHVGLGLSVD
                     SFYATKPGLMGKDFPSTIIGQMDLWRSAGALQLDMEAATLFVLSSLLGISAGAICTAG
                     SNIPKGLRPECPPSNDPAIRCACEAALLFKRWEKLSEQAGKNFLIPPIEDIGR"
     gene            12511..13281
                     /locus_tag="PLD15_02670"
     CDS             12511..13281
                     /locus_tag="PLD15_02670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB62832.1"
                     /translation="MGNRLENKVAVIFGGAGRIGSSAAEAFWNEGASLALIDKNAEKL
                     ATLKQRFLKGSGQEQRCVIIEDDIENESDAQTVSKRVREIFGKIDILLNCPAYIFRAP
                     FLDHPLEELDRQWHVNVRLVFIFSQAIAKVMKDGGRGKIINLASIGGQYPEKEHAGHC
                     AAKAGIIAISRVMALELAQFNIQVNVVAPGPTETVPFSSPFYTQHPEVLKRIESRTPA
                     GRIGHPEDHTGLLVFLACGESDWVTGQVILSDGGLGLV"
     gene            13498..>13993
                     /locus_tag="PLD15_02675"
     CDS             13498..>13993
                     /locus_tag="PLD15_02675"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPB62833.1"
                     /translation="MRIWLSFLFFLITAVSFAYVPDVLPPMEDPMLMTVWGESQELNT
                     VNYFCGSLFIARDYSPGATLEQMKMGAGLGKVEESEGDIFYHFYTPGTQYRTLIVIAG
                     GGSERHLGDISRVVEMAAEIKKENGLVMIIDIDVDDSGDDSIKAEFARRLIPFTDAVI
                     FAGSS"
BASE COUNT         3496 a         3503 c         3673 g         3321 t
ORIGIN      
        1 agatgcttag gaaaagcatc atcaaataca agaacaggca acttgaaacg gccaggatca
       61 tcgaggaatt gatagaattg gccaagatga tgcaacaggc tcgaaaacgt ggcgaagagc
      121 ttggactaac cgacgacgaa ctggcctttt acgatgcgct gtctgagaac aaaagcgcag
      181 tcgatatcat gggagacgaa aagctcaaag ttatagcggt gaagctctta gatacaatga
      241 ggaagagcat atccatcgac tggaccctta gagaaaacgt caaagcaaga ctgagaaccc
      301 agatcaagcg aattctcaaa aagttcggtt acccacccga tctgcaacag caggcagtca
      361 aaacggtgct cgaacaggcc gaatacatct gccgcgactg gacagggaat gagtgatcaa
      421 ctgatagcag tgaccaatgc ggttttcttg acgaaaaaga tgttcggtag aaaggagggc
      481 ggaataatcc ggaataatcg gtaatctaca acagactcct tttttccata ctcatactca
      541 caatctctga gaaggcaaag acaggatgca gatggagggc cacgtaaaca gggtcagact
      601 cctgttcttt cggatctgga agagaaagat atcttaagac tttcaatgac ctgcagcgag
      661 atgagaaacg aacaggagcg aatacaatcc ggtttttttc agtcaaaaga aatgaagtgt
      721 acaagagcta aaaaacaaca gacaaattgc gtgaatccct ttccagacct aaggttaaag
      781 cacctccgag ctactacgaa atccagcaaa acacctagtg acacgctcac aatcttcaca
      841 agttctataa ccctttcggg aaaatgaatt cgtgcatttt gttcgcccgt gtcatgtcga
      901 actcgggtag atcatttaat accatcgagt ctaacttttc taaaaaaaga cccggcagcg
      961 attcgccggg tctcttcgta tggttcggtt cgatctcagt aagtttcgca gaaaagttcg
     1021 aagtagcttt tcgggtgctt gcaggccggg catacatcag gcgcctcttt agcctcgata
     1081 atgtagccgc agttcccgca tttccacaac actttctctt ctctcttgaa gactttacac
     1141 ttctcgatat tatcgagcag tttcaggtac ctggcttcgt gtctgttctc caccttcgcg
     1201 atctgtctga agctgttggc gacatccttg aagccttcct tttcggccac atcggcaaat
     1261 tccgggtaca gcatagtcca ctcttcgttt tccccggccg cggaggcctt gagattctct
     1321 ctggtggttc tcagctctac cgggtaatcg gcatcgacat gtaccgttat cggcagttcg
     1381 ctggccattc cttcgaggat gtgtttgaaa aacactttcg cgtgttcttt ctcgttatcg
     1441 gcggtctcgg tgaaaatcgc ggctatctgc tcgtagcctt cctttttcgc gacggaagcg
     1501 tagaacgtat agcgattcct cgcctgcgat tctcccgcaa aggccttcat aaggttttcg
     1561 agtgtttttg tgcctttaag attcattgat tcacccccag gtttgagata tttttttggt
     1621 tttcttgtcc gatcggttgg atttgccatg gaaatcatta ctctttttat agtatattat
     1681 aagttacttt gttttgttcg atcaatccgg caagatagtt aattcttctc ttcaaattga
     1741 cctgccgagt cgattccaaa gttttcattt tcaacgtttc cggccggttc cacatggaca
     1801 tgcacatcgt acacgctctc attaccttca attatcgcct tctctacgtt cttcgccagc
     1861 gcgtgtgcct ttctgaccgt gagcagcgga tcaacctcta tatccagatc gaccaggtat
     1921 ttgtagccca ttttcctcac ccttaccttg tgaggattct gaacgccggg aactctggag
     1981 acggcttcga agattatgtt gtatatatcg tcaccgggtt tgagaccgtc catgagctcg
     2041 aagttggtct cccagaagat tccgacggcc gtttttataa ccatggccga gaccaccagg
     2101 ccgaccagcg tgtctattat ctggaaatcc gtgaagctaa ccagaataga gccgagaaga
     2161 acggacaggg atattgttat atccgatctc atgttcaggg catcggccat gaagaccgat
     2221 gagtcgattt ttctggctat cgccagcttg tatctgtata gcgcgtactt cccgatcgcc
     2281 gagatcaaag ccaccgtaac caccagcggc agattctcaa ccgacgagct tcccgaaaag
     2341 agcttctcga tggaactcat cgccagttga gcgccggcga aaaagatgac catcgatact
     2401 atcttagacg ctacggcgtc ggctctctca tgaccgtatg ggtgctgcgc gtccggcggt
     2461 ttgttggaga ccgaggtcga taccagagtt ataagggacg tcagtatgtc cgtcgacgag
     2521 tccatgccgt cggccagtat cgcgacgctt ccggtgagga aaccggtcaa caacttcata
     2581 accgccagaa tggcgttgcc gatcacgccg aagatcgatc cgatcctcgc tatcgaggct
     2641 ctatctttat tcattgagca cctctatcgc gctcgagagg cccgccaggc tccacctgcc
     2701 cctgagtctt tccagtgttc catttatcac aacgggagtt ccggccgtgt cgagcgtcgc
     2761 tctcgagctg cctgtgttat ggtacagaaa agcgtttcct ttgctcccgt ggaggttgat
     2821 ccttaccaga tccttctttc tgcttatact tttgatcgtg gtgaatctga gccagggatt
     2881 atccctcctg aatctcagta tatttttgaa acgctcgaag tagcttcccg ctataccgct
     2941 ctgcgcctgg aaggatacgc cgctgtgcgg gcggttcttt cccaaagcct tccattcggt
     3001 ctggccgggt ccatatccgt tttcgtacca gaggtgcggt tccagctgct cttcgttgaa
     3061 gtactcgtcg tagacccctt ccattcccag ctcttctccg tagtacagga agggcagtcc
     3121 cgggagagtg agcagcaccg taagcgccag aagcgccaga tccctgtcgc cattcattga
     3181 gctgacgagc ctcgtcatat cgtggttggt caggaaagtg ccagactcgt aactgcgtct
     3241 ggatctgaaa aatctctgca tgtccgtctt cagagccttt acgagcgaca cggcgttcct
     3301 ggaagctatc gccagcttca gatcttcggc cagggggaag ttgaaaccga tcccgaagat
     3361 gctcgagtac atatccacga ttcgggcgtc gtcccagtcc tcgctaacga agaggcaatc
     3421 gggggagatc gcgcggcagt gggaagtcat ctccttccag aaggcgatgt tcctgctgtg
     3481 ctgatactcg caaatgccct tctcgatcga gaaatcgaag atgtgtttgg ccgcgtcgaa
     3541 cctgaaccca tcgaatccca ccgaaagcca gaaagtcaaa acctctttta tctgttgcca
     3601 tagagaagga gactcgaagt tgagatcggg actccccggt ccgaagagcg catataccca
     3661 ccggcccgag tagttagacc agacctgttg attatcccag tggcggcgcg ccttaagcca
     3721 ctcttcactt tgcagaaaga gataccagtc gcggtacggc ttctcgccgt ccagggcttt
     3781 caaaaaccat tcatggctgg gcgatgtgtg gttgagcggg agatctagaa ttactttgag
     3841 ctctctttta tgcgcgccgt aaaggaagtt tctcaagtcc ttcaggtctc catagatcgg
     3901 atttatggcg aagaaatccg ttacgctgta cccgtggtaa gcgggagatt gcattatggg
     3961 aagcagccag attccttcca tcccgagttc ggctatgtag tcgagcttct tttcggctcc
     4021 cttcaggtcg cctattccat cgccgtttcc gtcgtagaag gaacggagat aaagctcata
     4081 gaacaaagtt cttccctcca gtcagcatga attaatttgg accggttaac tcaacattag
     4141 aatcttacat cttatcacag ttgttgggtt atttcttgtg atagaatttt ctctagcccc
     4201 ggtagaagga gatggaatcg aatgttgatt gaagagttgc tcggcaaggg cgaaaagatg
     4261 aacgccagcg atgttcacat aacctgtaga gggaacgtta tgtacagagt ggatggagaa
     4321 cttgtccagg atgtatttct gacggacgat ataatgatga ggaagatcct ctcggagctt
     4381 cagggcctca ccaacgaaaa gttcgaagag tcgggaaaga aagagatcga tttctctttc
     4441 gggttcggtt cctcgcgggt gaggggcaat ttcttctatt caaacaagct gccggcgctc
     4501 gcgctcaggt taataccgaa aacgataaga acgctcgacg atctcggata ccccgagctc
     4561 ttcaaggaat tctgtaaacc tgacaaagga ctggttctgg tggccggtcc caccggaagc
     4621 ggtaaatcta cgacgctggc cgccatgctg gagaacgtca atcgcaccag gcatgttcac
     4681 ctcataacca tagaagatcc ggtcgagtac gtcttcgagc agaagaacgc gttgatccac
     4741 cagagagagc tgggaccgga cacgcaatct ttttacaacg gtctgaagta cgccctcaga
     4801 caggacccag acgtgattct cgttggagaa atgcgagaca gcgagaccat ggaactggcc
     4861 atgaccgcgg ccgagacggg acatctcgtc ttctcgacca tacacaccaa ttcggcggcc
     4921 acgacgcccg aaaggatcgt cggcgttttt ccgccccacc agcagaacca gatatccatg
     4981 cagctggcca acactttgat ggccgtcgtt taccagagac ttctcaagag acgcgacggt
     5041 aagggaagag tggccgtcat agagatcatg gtggtcaatc aggcgataag aaatctcata
     5101 cgtgaacaga aattccacca gatagagtcg atgatgcagg ccggggtgaa attcggtatg
     5161 gtcacgttcg acgatgccct cgtggagatc ttcaagaagg gaataataga caaagatcag
     5221 cttctgtcct acgcgaggga cgccggctcg atagccagga ggagtttttg atgaatcttg
     5281 aaaagatcat agaagtcttt cgtgaaaggt tcaaagaggt cgaggctcag ctttccaggc
     5341 ccgaggttgc ctccgatccg gaaagactta tggaatacgg caagaaacac gccgggctct
     5401 cccagataat cgagcttcac gagaggatag agaccatgaa gagagagctc gacgactggg
     5461 gcggaatgaa gaacaacagc gaagaagaag agctcgaaga gatagagagg actatcgacg
     5521 aactgaccgg gaagatcgcg aaagaagagt tgaacctcaa ggcgcttctg gttccagatc
     5581 tggccgacga caggaacata ataatagaga tcagggcggg aacgggcggc gaagaggcgg
     5641 cccttttcgg ttccgatctc ttcagaatgt acagccgcta cgcagaacgc aacaactgga
     5701 agtctgaagt tatcgatgtg aatgaaaccg atcttggcgg cctcaaagag atcgtcttcc
     5761 agataaaggg ccggagcgtt tacagtaaac tcaagtacga gagcggcgtt cacagggtgc
     5821 agagagtgcc cacaaccgaa tccggcggga gaatacacac ttcgaccgcc acagtcgcgg
     5881 tactccccga agtcagcgag accgaggtca agatagagcc ttccgatcta aggatagaca
     5941 catacagggc ctcgggggcc ggcgggcagc acgtaaacaa gaccgactcg gccgtcagga
     6001 tcgtccatat ccccaccgga atagtggtgg cggtacagaa ggaacgatcg cagcaccaga
     6061 acaaggcccg ggcgatggag ctgttgaggg caaaactgta cgatatgtac agctcgaagg
     6121 ccgagaacga ggtaacccag accagaagat cgcagatcgg cactggagag cggagcgaaa
     6181 aaatccggac ctacaacttt ccgcagaaca gggtgaccga ccaccggatc aatttcacga
     6241 gctaccgcct tctggatata atggacggag atattgagga actggtcacg gaactctccg
     6301 ccgccgatat aaacaagagg ctcgaactcc ttcacaaaga actggtgggc tgagccggaa
     6361 acggccctcg agtgtgaagg tcacatttcc agctttgcca gaacccacat gttcagcccg
     6421 taaagcaacg acccgacggc cgcgagcgag atgaaggcga tgccgaagga aaaggcgtcg
     6481 gccatctttc cgaacgacgg gaaaatggct atcatgacca cgctgaaagc catgctggaa
     6541 aacgagatga tcgtcgccct gtactgggac ggtatgagcc tgtttatgta atcgctgctg
     6601 gccacgaaga gcatggcttc aaacatggat atgagaatga aaaagggcgc cttgaacggg
     6661 gagagggcga gcgcccacat ggaaatcact gccgccagcg gcaaaaggat gagcatcatc
     6721 ttctccctga gcagcctctg gagtttgtga acctttgtgg ccgtcactcc ggcggcaaga
     6781 gagccgatag cgagcaacac cccgatctgg aattcattca ttccctctcc cttccagaag
     6841 ttctgcatgt agaagaagat acaggtgcaa aaagccagta tggtttgcgt aaagagaatc
     6901 aggaaggcca cttttctggt cttcgcaatc acgccgaagc tgtctgcgat ttgccttgca
     6961 aaacccttca ggctacgctt tggcgaagat ccggccgaat ctgaatctat aggcggttcc
     7021 acgaaaagca gagaatagcc gatggtgaag attattatcg ccgccgacac ccagaaggcc
     7081 aggtgataat cgaaagttcc gaggagaccc ccgacgagga aggccgcgat cgatgagcac
     7141 tgaaagacgg cctcctgttt acccgcaatc cgcatgaact cttcgctctt tccgagatat
     7201 ttcagggaat catagaccag agcctccccc gaaccggatt ccagattgta cgaaagtgca
     7261 gaaaacacca tgaaaaaaag aaaatgcgtg aaatcgctgg agacgatcaa aatcagaatg
     7321 ctcgcaagag acatgcaacg tcccagggtg cggctcagtt ttcttccaaa aagatccgcg
     7381 atcgaacctg taggtacctc catggccagc gaagtgacgt ggaatatacc ctccagcaat
     7441 ccgatctgcg tcaggctcat tcccttgaag gccatgtaga tcatccacag accgcccgac
     7501 agtctgaggt tcatgaagaa agagaataca tagttcctgc gtatgttttt ttccattcca
     7561 acagccagtt tatccattta aaacctctcc cgaaagaaaa ggccgtgact cccgtcacgg
     7621 ccttgtatac tgatgctttg aggcggttta agctccggta ccgagcccga cgaggtagca
     7681 ttcacccacg gaatcggaaa ttccgccgag gatcgttctt ctgtgagcct ggataatatc
     7741 cagcatgagt tgccacctcc tctttatctg agagaaatta tacactccgt ccgggcgatt
     7801 ttgtacataa tttccttttt cgacgcctcg aaaagttgca agagttcgga gaattctgat
     7861 atagtatggt tgtaggacat cctacatctt gatcgggagg gttgcacatg tctaacattc
     7921 ccgtagagaa ccgcggtcac agctctttga tcagtattat tcaaaacgaa ctcaagacca
     7981 ggataattaa cggagccttc aagccgggag agaaacttcc ttccgagccg gagctcgcga
     8041 tggagcttgg ggtgagcaga aattcattgc gagaggcgat cggtcttctc cagagagagg
     8101 gcctcctggt gaagcgtcac ggtgtgggca acttcgtgac cgacaggtac cctataatcc
     8161 gcggaggaat tgaacgactc gccgggatca ccgagttcat acgggcgcag gggctcaagg
     8221 ccgggagcaa agtcaccaga ttcgagctcg ttgaatgccc cggggagatc ggccagacgc
     8281 tcgatacgga accgggtgac aggatcttca taatggaaac gatcaagtac gcctcggaag
     8341 tgccggtagc gctctgtgca gatttcattc ccctctcgat agccggaaac atcgatccgg
     8401 ataggattca tgactccatc ttcgacggtt tgagggatca ctacggggtg gatataaggt
     8461 acgccgagtg cgatctgata cctatgaccg caggagagga attgaccgga aaactctccg
     8521 tcgaagccgg aacaccggtt ctgctgctgg agcagataca ttacgattcg ctgaacagaa
     8581 gggtcctgta ctctaaaagt tatttccctg tcggtaagtt cactttcaag ttgatcagaa
     8641 agcgctaaga taccattacc aaggaggtga atgcgttgag aaggcttttt gtactgctga
     8701 tactgtttat cgggatcttt ctctctgcga cgaccatcga ggttttgtgg atgggctggc
     8761 cgcaggacca ggttatgcag ctggtgaagg ctttcaagga cgagaatcca acggtcgaag
     8821 tggacattca acttgttccg ttcgggcagc tgttccagac tctcgaagtt agactcgccg
     8881 cgggcgacgg gacgcccgat gtctatatcg ttgacggtcc taacacagcg tcttacgcgg
     8941 ccagaggtta cttgctggcc ctggacgatt atttcacgga tgaagagatg gatgactggt
     9001 ttagcgcatc gatcgaagag gggagctacg acggcaagtt ttattcggtg ccgtacggca
     9061 cgtcttctgc cgggattttt tacaacaagg ccattttcga ggagtacgga attccctttc
     9121 cacccgaagg gatcgacgag agattgacat gggacgaggt tgtcgaaatc gccagagccc
     9181 tcaccaaaga cacgaacaac gacggtataa ccgacatctg gggtctggtg atcgagcaga
     9241 tagacagacc ctatctgata ttcccgatcg tgcaatcgct gggagcccgg gcgctctcga
     9301 gcgacggatt cgtctcgcag ggatatatca actccaaaga gttcatagaa ggcacgactt
     9361 tctactggaa actcttcaac gagtggaaga taagcccaca gggcgttcct gattcggcca
     9421 tcgccaggga gtatttcggc accggcaagg cggccatgat gctgggcaac gaatggaata
     9481 tacgcaggat gtcccagtat ccctcggtcg aattcggcct gacgcccttc ccttatttcg
     9541 aaggaggagc gatcgtcact cccaccggaa gctggcatgt gggaataaac tcgaaaacca
     9601 ggaagctcga cgaagctctg aagttcacca agttcattac cggaaaagaa gccgtcataa
     9661 cctggcacat gctgaacggc atcgccccgg ccaggtacga tgtctatgag gccctgcccg
     9721 aggtcttcgg aaatccgatg tggcagacct ttctgtatga gatggaacac accgccgtgg
     9781 ccagacccgt aacgcccggc tactcgcagt acgaattgat gctcagagag gctttcaact
     9841 cgattcacta cggcgccgat cccaagaaga ccctggacga tgccgcaatc aggatcgata
     9901 gagaactgag aagatacaag tgatcgaacg gaatcgtatt tgaattcatt ctggatggtg
     9961 cggaagtgtt caggaatggc agagttgcat ggctttttat attacccgcg ctggcatttc
    10021 ttttgttgtt caagatctgg ccgataggcg tctctttcat cgagagtctg acgacgacca
    10081 gtttcagagg aacaaagtct tttgtggggt ttgaaaatta ctcgtatcta ttcaggtacg
    10141 atcccgtttt ctggaattcc ttctgggtaa ctctcctgta ttcggcgata gtcaacccgc
    10201 tgatagtcct gagttccttg ctcatggccc tgttgctcaa cgggtcgtat ttcttcacaa
    10261 agttttttag aacggtcttc tttctgccca cggcgatctc gtacgcggtg gtcgctgtta
    10321 tctggtcaat cgtgctcgat ccttactacg gactggcgaa cagttttttg acctctttcg
    10381 gtcttaagcc gcagcctttt ttcgcctccc ccgatcaggc gctgtgggga ttgataatgc
    10441 tgaacgtgtg gagaagctcg gggtactgga tgatgtactt cctggccggt ctgcagaaca
    10501 ttccggaaag tctctacgag gcggccgaga tagacggcgc ctcgaccctc cagaagactc
    10561 tgaagatcac cctgccgctc ctgacgcgaa cgatgtcttt tgttctggtg gccaacacgg
    10621 ccttcaattt tctcagtttc gctccggttt acataataac caaaggcgga ccgatgggct
    10681 cgaccaacct gctcatgtac gaatcgtaca agagcgcctt cgtgaacgcc gatatgggca
    10741 gggcgtcggc cattacgtcg atacttcttg cgatcattct cgtcttcagc ttcttcgaat
    10801 tgaagctgac taaggcgaat ttcgagtatt gaggagagat tcatgaaaag tggcagactc
    10861 tggaaaacaa catcgctttt catcgtactg gcggtcgtca gtctcttctt catagtaccg
    10921 ctggtgtgga tagtggcctc ggccttgaga ccggccggat cgctttacca gtacgccaat
    10981 cccctcacct gggggacttt cataccctcg aaaccgacgc tggagaattt cgtacacatc
    11041 ttctcgaagc tcaactttgg cagggcgatc gccaacagcc ttttcgtttc gctcgttacg
    11101 atactgctca cggtcttcgt ttcctcgatg gcgggatttt cgctggcgaa attcaatttc
    11161 agaggaaaga acctggtctt tgccatagtg ttgataacct tcatggttcc gttcgaatcg
    11221 atcgtgattc cgctctatat tctcgtgaaa cagctcaaaa tagacaatac ctattgggct
    11281 ctcatacttc cgggcgtggc gaacgggctg gcgatattcc tcttcaggca gttcttctcg
    11341 gaagtgcctt cggaaatcat ggaagcggcg cgaatcgatg gcgcttcctg gttcaggatc
    11401 tactggagga ttttgctgcc cctgagtatg cccgcgatag tgaccgttat cgtcatggtc
    11461 ttcatgttcc agtggaactc ccttttctgg ccgcttgtcg cgactcattc cagcaggttt
    11521 gaagtcgtgc aggtggccat tgcttctcac agatctaccg aaagcacgag ctgggccaat
    11581 ctcttcagct ctgcaatcgc cggtagtctg ccgccggtaa ttctattttt gtttctccag
    11641 aagtacttcg ttcgcggaat aagtggaacg gggttgaagg ggtgaacgca tggaaagcat
    11701 aagtatatcc aaggaccatc tcaagctctc cgtagacaaa ctgccggaca ttttgcttct
    11761 gcccggcgat cccgcaaggg tctatgaaat aggacgcttt ctggacaacg ttgaagaggt
    11821 tgggaacaac agagattact tgaccatagt cggcgagtac aaagggctcc cgatagcggc
    11881 ctgttcgagc ggcatgggag gtccatcgac tgagatagca gtcgttgagc ttcacagaca
    11941 cggcgtgaag agtattataa gaatcgggac ctccggcggc ctttcagaaa agatctcgcc
    12001 cggcgatttg atcgtccttt cgggctgcat aaagtactcg ggaacggcca acctgttcgt
    12061 gccggagaat tttccatcga tcgccgacta cagggttctt caggcgctga tatgcgcctg
    12121 cgacagagaa gaggccgcct ttcacgtcgg tctgggcctt tcggtcgatt ccttttacgc
    12181 gacgaaaccg ggattgatgg ggaaggactt tccttctact ataatcggcc agatggacct
    12241 gtggagatcg gccggcgcgc ttcaactgga tatggaggcc gccacgctgt ttgtactgtc
    12301 gtcacttctg ggaatctccg ccggagcgat ctgcaccgcc ggctcgaaca tacccaaagg
    12361 tttgagaccg gaatgtccgc catccaacga tccggctatc aggtgcgcgt gcgaggcggc
    12421 gcttctattc aagagatggg aaaaactttc tgaacaggcc ggaaagaatt tcctgattcc
    12481 accgatcgaa gatattggga gatgataaca atgggaaaca gacttgaaaa taaagtggcc
    12541 gtgattttcg gaggcgccgg aaggatagga agctcggccg ccgaagcctt ctggaatgaa
    12601 ggcgcttcgc tcgccctgat agacaaaaac gccgaaaaac tggcaactct aaaacaacgt
    12661 ttcttgaagg gctcagggca agaacagcgc tgcgtcatca tcgaggatga catagagaac
    12721 gaatcggacg cacagacagt ctcaaaaaga gtgagggaga tctttggaaa gatagacata
    12781 ctcctcaact gtccggctta catattccgc gcgccttttc tcgatcatcc attggaagag
    12841 ctggacaggc agtggcatgt gaacgtgaga ctggttttca tcttctctca ggcgatcgcg
    12901 aaggttatga aagacggagg cagagggaag ataatcaatc tggcctcgat cgggggccag
    12961 tatccggaaa aggagcacgc cggtcactgt gcggccaagg ccggtataat agcgatttcg
    13021 agagtcatgg ctctcgagct ggcgcagttc aacatccagg tcaacgtcgt ggctcccgga
    13081 cctaccgaga ccgttccctt ctcctcgccg ttctatacgc aacacccgga agtcctcaag
    13141 aggatagaat ccagaactcc ggccggaagg atcggtcatc ccgaagacca caccggtctg
    13201 cttgtctttc tggcttgcgg ggagtccgac tgggttaccg gtcaggtgat actttccgat
    13261 ggaggattgg gtcttgtgta aatatgtaaa taagagtcga tttctaaatg tggctctgtg
    13321 tcggcaggag ctaactggat aaaaagactc ctttttgaaa cttctgtatc aaaatcggga
    13381 aagttcgcta ttcgaagtat ttctctgata taatttggag tatagtaatc taaacgatga
    13441 tcctgtattt ttttctgtcc gaaattcaca ctggtcggtt ttagcggaga tggtcttttg
    13501 agaatctggt tgtctttctt gttttttctt ataactgctg tttcttttgc ctatgtaccg
    13561 gacgttctac ctccaatgga agaccctatg ttgatgaccg tctggggaga gagccaggag
    13621 ctgaatacgg tgaactactt ctgtggcagt cttttcatag cccgtgatta cagtcccgga
    13681 gcgactctgg agcagatgaa aatgggcgcg ggcttgggca aggtggaaga aagcgaaggc
    13741 gatattttct accatttcta tacgcctggc acccaataca ggaccctgat tgtgattgcc
    13801 ggtggtggat ccgaaaggca tctgggggat atctccagag tcgtggaaat ggcagccgaa
    13861 attaagaaag aaaacggact ggtaatgatc atcgatatag atgtggatga ttccggtgat
    13921 gactcaatca aggcggagtt cgcccgaaga ttgattccgt ttacagatgc cgttatattt
    13981 gccggtagtt cta
//
LOCUS       DAOOLY010000029        13991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4731_length_13991_cov_4.138634, whole genome shotgun sequence.
ACCESSION   DAOOLY010000029 DAOOLY010000000
VERSION     DAOOLY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13991
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4731_length_13991_cov_4.138634"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLD15_02680"
     CDS             complement(<1..828)
                     /locus_tag="PLD15_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPB62834.1"
                     /translation="MNKYLKALQALAVGDAMGMPTEFMTRDTVRRFGLVSELLDPVVS
                     PIHPNLRKGSITDDTEQNLYLIDVYCKSGEITVEGTANALICWVRETAADSRGYIGPS
                     SLKALKAIESGEDPRKTGRGGTTCGAAMRAPAAALCSKNDSPEKLARDIYNCSLPTHN
                     TNLAVEAALAMGFAVDAAAGGAGVEEILKASLEGASCTNDLDTPNYVGASSAWKIKFL
                     IELLADKSEIDEILDTIYYLNGTGLPANEVVPAALAVFSVAKGDVWLSIRMGASVGGD
                     "
     gene            complement(846..1814)
                     /locus_tag="PLD15_02685"
     CDS             complement(846..1814)
                     /locus_tag="PLD15_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62835.1"
                     /translation="MRVSVLFNPGAGKSDRFAAIAGEILDRFEGHTLFTGEIDFGEIL
                     LKKADISVVRTSRVSFVETLEAYLTGFFERNTDLVIGVGGDGFLAHIASFLIENGLQT
                     PLMGIAGGTANVGPLIGYDIDRLRKFSPENLRIESITALIAKGSREKNCYAFNDIVVG
                     DTFLGTIDGIMVNLSAADFIRDLSKIEIRPSESIATENFRILKNGRPVPPPPFKVSQI
                     VVSPLFKKEFYAGKAVTGALCWAPYFSSGAAVSVMDTIVIDSQLEGPDSAVCIQHILF
                     DENDLIEIDGLSSYLIADGNVCYKINETVSVRCLKGAVKSVRPTLD"
     gene            complement(1828..2601)
                     /locus_tag="PLD15_02690"
     CDS             complement(1828..2601)
                     /locus_tag="PLD15_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPB62836.1"
                     /translation="MRISFSTAALYPRPSLEALFLLGKTGYGEAELMPQCMEETKPEF
                     AKETAKTGIWVSSIHFPLVFFSVLYNPYPGMMKEARIMAEEMARSAEIMKSEVVVVHA
                     LPKMDAIKARLFEEPVIEVLRTLADKLKLAGARLAVENNPSTLGDTPQGLLESLEKIN
                     HVNAFPMVDTTESWEAGIDPAYFIRLARPIHLHLSDHAGDQKHIPAGQGEGDWKAILS
                     ALKEIDYSGIYVLEPAYRHYQEDVEAKLRLARKSLDIFL"
     gene            complement(2598..3833)
                     /locus_tag="PLD15_02695"
     CDS             complement(2598..3833)
                     /locus_tag="PLD15_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62837.1"
                     /translation="MKKVLLLIVISVAAISLAVTEITFWYALSGVSGESIKSIVDAFN
                     ASQSDITVKTVYSGSYADTAQKITAALAAKALPNGGIIPAGPIFTGAQDNYVLLDYIL
                     ADPEFDMDDFYPAMWDYSKYQGKVCAIPFNISTPIFYYNKDLMKASGLDPENPPQNWE
                     ELLTAAKAITKDTNGDGVPDIWGVDMADAPWIFKALLLQNENDIIDVATMTPLFDNAR
                     GIETAQFWKMLIDEKAMPVGQHSLAEKMFLGGTLGFYLGSSNRIGRWLGTTSFEFGAT
                     FLPAGAVRAVPIGGAVAVLFPKNAKEDEATYRLIKWLTTPENVAKFAMETGYLPTRKS
                     ALELPETQQFMESTPMWRVAFEQLTFAYSYWHFNEMGTMDTMISETLEKIERGVSSPD
                     DAMKTLTSDLKAEIEANLE"
     gene            complement(3848..4687)
                     /locus_tag="PLD15_02700"
     CDS             complement(3848..4687)
                     /locus_tag="PLD15_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB62838.1"
                     /translation="MTKKGLKGERVRNRVAFILLLVGLFAMLFPLFWMVLSAFKTKAD
                     VYSYPPRWFPSSWSLDNFAKVFEMVPFGRYYFNSILVTSLSTLGQIIVSILAAYSLAR
                     LRFPFKNLIFMFVVVTMLMPFVVTMIPTFLIISSLKWIDTYQGLIVPFLFNGFSIIFL
                     VQFFITVPVDLQDAARIDGCGYFGILSHVILPNTKPAISTIALFTFLGHWTEYLWPLI
                     VINTTEMRTLPIGLRYLMTEGSSEYQLMMAASVMAIVPVLLVYMFSEKQFIKSITMTG
                     LKS"
     gene            complement(4684..5583)
                     /locus_tag="PLD15_02705"
     CDS             complement(4684..5583)
                     /locus_tag="PLD15_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62839.1"
                     /translation="MPKKIRQRYNPREVAVAFICLAPALFLATVFVVIPLVNVIYLSF
                     TNWDLLRATKKFIGFSNYQYIFKDEKFLKSILNTLYFAAVKIPLDLVVSLFIATLLDR
                     KVFARRFLRASYFAPVVLPMVAASLIWIWIYDPSLGPLNQILGLFGVKPIRWLYDPEW
                     AMPSVIFFALWKGLGYDIVIFLAGLQAIPETYAEAAKIDGASDRKIFFRITLPLLSPV
                     IYFVILMGIINSFKIFTPVSVMTPTGGPLYSTGVMVFYIYQQAFQNYRIGRASAAAVV
                     LFLMILSLTWVQRKAGSKKVIYE"
     gene            complement(5589..6548)
                     /locus_tag="PLD15_02710"
     CDS             complement(5589..6548)
                     /locus_tag="PLD15_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB62840.1"
                     /translation="MKALFYLGPGKLELREIEKPAGDIVIRVLGAGICGTDLKTYLKG
                     HPMFKPPTILGHECYGRIDAINNYRGVLKVGDIVAVAPYLECGKCDSCKRGVPELCKS
                     KTYVETGCFSEYISMSEEHALKGLFKAEDDPLYSLAEPLACVYNGFEKLGRKPEKLLI
                     VGGGPMGMLLALVGLSEGCETRIVEKSEWRLEYIRRAGIEGSAERPDGFFDSVVLAVN
                     IPELVGEYTQLVADGGSLLLFSGYPKDSTINVDPYAVHYREVSITGSFGFGFRHFERA
                     VGNLYANRDLFGKLITHRFDMRNAREAFELLNRGRAMKIIFGM"
     gene            complement(6545..7480)
                     /locus_tag="PLD15_02715"
                     /pseudo
     CDS             complement(6545..7480)
                     /locus_tag="PLD15_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
     gene            complement(7513..8283)
                     /locus_tag="PLD15_02720"
     CDS             complement(7513..8283)
                     /locus_tag="PLD15_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62841.1"
                     /translation="MHKCAYIFHKDVIDLTGLERNVASLFSNGGHLFVVALDHPQFFG
                     PVEGIDKPAELIERLSMSRADGFIVNPGITRLLNARTVSGKKLIVRSSIGGSKFSDYK
                     TFHPAVVSAESLLDLGADAAILMLVLGGRDYDSMENVARAIDEYHSLSIPVVVEVLAE
                     DFNKTSDPDLVKTGARIAAELGADVLKVFYTEDFSVVVQGCPVPVILAGGPRNLDVLT
                     MARNAVNCGVKGFAFGRNIFQSEDPVKLIAELDGIIRG"
     gene            complement(8406..9647)
                     /locus_tag="PLD15_02725"
     CDS             complement(8406..9647)
                     /locus_tag="PLD15_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62842.1"
                     /translation="MRLKRVVLVMIVLATVFFLLSACFPKDPLEAATKKFIELIKKEG
                     EPEDTFVGVRLAEVAVGDIITSETADGGQEFQYQLQGLNQAGWLFYLDEAPGAFYDHP
                     GRLVVISKNGQKIFDQKIEGVPKVNDELPAPLLTRSQYIASTIWDKSKIVYLPIIDWI
                     DVIIARIIRKGAVITSGLTPTQNLYAEARDARDLMSTSFKTLMGADKVRDVKYTAGGP
                     APNWTAVQNAMNDLFTNEKINHMTLYFVAHGNINLMNLGGTTFYASQLRSYIQEHANT
                     TFCLIIESCHAGSWLDGLKSGGVMPANIEIAITTTSSSKSAYPDWDHVGGVLADFNPS
                     DVYVEWSGDFLQKMAYFTSDAHWPEVQTYATTYSTSDIAALLYKCFTAIKGATPQTTS
                     LTLTERTIAGFIQDPKVYRDW"
     gene            complement(9908..10795)
                     /locus_tag="PLD15_02730"
     CDS             complement(9908..10795)
                     /locus_tag="PLD15_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HPB62843.1"
                     /translation="MKKLLTVSTIILLAMVLFAQSEFMIPLSTADFALKGPVKSMKTV
                     YPPSDVDLGEDVVMPAYEVISFDEAGKIVSQISYDKDGKEINAILYGYDAFGKLSAIS
                     GRENGEEQIIGELTIEDGKIISIVADEGEDKSTINMEYDENGRLVAQIISGMSEGQEI
                     QIAVNMAYDERGNLIEESMSMMGMVLSKTVFEYDSNNLKIRELEYMYMFAEPGTEPEP
                     IASQFEYNEWGDVFKKISDSFFSDDKEVTVYEYEYDAAGNYTHMTVYFLYSIQDLEKE
                     NWKELATVESEETREIEYY"
     gene            10883..11791
                     /locus_tag="PLD15_02735"
     CDS             10883..11791
                     /locus_tag="PLD15_02735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB62844.1"
                     /translation="MYYIDEATPQELNEIRTRMYKAIPESSNGRYSPGELRPVDEVYR
                     DFLNAGDSVAVMRCRGTIVGYIHFGRRREETYSPEETGHIFDIFVEEAHRKRGIATQL
                     LDHALSRMYSQGYGKVSGNTFRGGPGWEILLKRGFRPVTVCLSIESRSPVREESGVSL
                     GVPADLLMFSEGLTISRAELAQDLSMNLSELYYRLNAVIERGGTLLIDSHGGSPAGFL
                     VYSVFEDLMTYDFLGYLDFVFVVHPFRRLGVAGSLLYESLRRLKGVQAREMFFECNCD
                     AYCYKWALSVGMKEFSILLEKDLDRL"
     gene            11933..12568
                     /locus_tag="PLD15_02740"
     CDS             11933..12568
                     /locus_tag="PLD15_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPB62845.1"
                     /translation="MELLERIKNAIIETMPVMAKQATQEALEKGLSAKDILNSALIPG
                     MDEVGRLFRGGEYFVPEVLVAAKAMNASMDLIEPLLVGSGVRKVGKVVAGTVEGDLHD
                     IGKNLVCMMLKGAGFEIIDLGVDVKPESFVEATKREKPDILVISALLTTTMLNMPKVI
                     NALREAGVREKVKVLIGGAPVSRDYAEEIGADGYSEDSSSAVELARKLVTV"
     gene            12794..>13991
                     /locus_tag="PLD15_02745"
     CDS             12794..>13991
                     /locus_tag="PLD15_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPB62846.1"
                     /translation="MEKNYSSLYNALVDHPDLLICRWLPDTSLTFVNDTYARFYGYEK
                     SEMIGKKWLEISKIDIQEDLRLSVNRAEALRDSIVREDPAIDPEGKEHWVRWYNRPII
                     DDSGTVVEFQSIGIDITDQRQITQKLLESEREMSLVLDNIEELVAYHRPNLSLVWMNR
                     AYARSCDSSQRDLTGRFCHEIWYGSKQPCENCSVALAVRERKNVTGEITSGERVWHIK
                     AYPVFGQDGELLGVVDFSYEVTEERKLSEELIRSEERFKRLVNSTDDIIFELDREGRH
                     TAIHGRWLEKMGFAEEFFLGRSAGDIFSAEDALVHEQNNAIALGGKSTKYEWSTEVDG
                     IHIFYNTSLSPIFDREGNVTGIVGIGRDITDIKRTEETLKRSWDQLIMTLSRMLRIKD
                     PYTAGHQ"
BASE COUNT         3348 a         3505 c         3608 g         3530 t
ORIGIN      
        1 gtcgccgccc accgaagcgc ccattcttat tgaaagccag acatctccct tcgctacgga
       61 aaaaaccgcc agagcggccg gcaccacttc gttggccggc agtcccgttc cgttcaggta
      121 gtatatcgtg tcgaggatct cgtcgatctc actcttatcg gcgagcagtt cgatcaagaa
      181 cttgatcttc caggccgacg aagcgcccac gtaattggga gtgtcgaggt cgtttgtgca
      241 ggaggcgcct tccagagagg ctttcagaat ctcctcgacg cccgcgcccc cggccgccgc
      301 atcgacggca aagcccatcg cgagtgcggc ctcgaccgcg agattggtgt tgtgggtggg
      361 aagagagcag ttgtatatgt cccttgccag cttttccggc gaatcgtttt tactgcacag
      421 agcagcagct ggcgctctca tcgccgcccc gcaggtcgtt ccgcctctcc cggtcttgcg
      481 aggatcttcg ccgctttcta tcgctttcaa ggccttcaaa gagcttggac ctatgtagcc
      541 cctggagtcg gccgcggtct ctctgaccca gcatatgagg gcgttagccg tgccttcgac
      601 cgttatctct ccgcttttgc agtagacgtc gatgagatag aggttctgtt ctgtgtcgtc
      661 ggtgatcgat cccttcctga ggtttggatg gatgggggaa accaccggat cgagaagctc
      721 cgaaacgagt ccgaacctgc gtacagtgtc tctcgtcata aactccgtgg gcatgcccat
      781 ggcgtcgccg acggccaggg cctgaagagc cttaagatat ttgttcaact ggctaattct
      841 cctttctaat cgagggttgg tcttacgctt ttcacagctc ctttgaggca tctgacagag
      901 accgtttcgt tgattttgta gcaaacattg ccatcggcaa tcaaataaga agacagaccg
      961 tcgatttcaa tcaaatcgtt ttcgtcgaaa agtatatgtt gaatgcaaac ggcgctatct
     1021 ggaccttcca gttgcgagtc gatcacgata gtatccataa ccgaaacggc cgctccggac
     1081 gaaaaataag gtgcccagca taaagcgccg gttacggcct tccccgcgta aaactccttt
     1141 ttgaacaggg gagatacgac tatctgcgat actttgaaag gtggtggtgg aacggggcgg
     1201 ccgttcttga ggatcctgaa attctctgtg gctatacttt ccgatggtct gatctctatc
     1261 ttcgagagat ctctgatgaa atcggccgca ctcaggttga ccattatccc atcaatggtt
     1321 ccgagaaatg tgtcgcccac gacgatatcg ttgaaagcgt agcagttttt ttcccggctg
     1381 cctttcgcaa tcagcgccgt aatgctttcg atgcgcaggt tttcgggact gaacttcctc
     1441 aaccgatcga tatcgtaccc gatcagtggg ccaacgtttg cggtgcctcc ggctatgccc
     1501 atgaggggag tctgcaatcc attttcgatt agaaaagagg cgatgtgggc gagaaaacca
     1561 tccccgccca caccgatcac gagatccgtg ttcctttcaa agaaaccggt gaggtatgct
     1621 tcgagagtct ctacgaaaga taccctggaa gttcgcacca cactaatgtc ggccttcttc
     1681 agcagaattt cgccgaaatc gatttcgccg gtgaacagag tgtgaccttc gaacctgtcc
     1741 agaatctcgc cagctatcgc agcgaatcta tcgctttttc cggcgccggg attgaacagt
     1801 accgagactc tcaacaatct gcctccttca aagaaagata tccagagatt ttctggccag
     1861 cctcagcttc gcttccacat cctcctgata gtgacggtaa gccggttcaa ggacgtagat
     1921 accgctgtaa tctatctcct tgagggccga gagtatggct ttccagtcgc cctcgccctg
     1981 ccccgccgga atgtgttttt gatcaccggc gtggtcgctc agatgaagat ggataggcct
     2041 tgccagcctt atgaaatacg ccggatcaat tcccgcctcc caagattcgg ttgtatctac
     2101 catcggaaaa gcgttgacgt gatttatctt ttccaacgat tcaagaagac cttgcggcgt
     2161 gtcaccgagc gtgctggggt tgttttcgac tgccagcctt gccccggcaa gcttgagctt
     2221 atcggccaga gttctcaaaa cttcgattac aggttcctcg aaaagacgag ccttgatcgc
     2281 gtccatcttc ggtaaagcgt gaaccacaac tacctcgctt ttcataatct ccgccgatct
     2341 ggccatctct tcggccatga tccgggcctc tttcatcatt cccggatatg ggttgtagag
     2401 aacggagaaa aagacgagcg ggaaatggat ggagctgacc catattcccg ttttggcagt
     2461 ttctttcgcg aattccggtt tcgtctcttc catgcattga ggcatcagtt cggcttcacc
     2521 gtaacctgtc ttcccgagta gaaagagcgc ctcaaggctc ggcctgggat aaagtgcggc
     2581 agtcgagaac gagatcctca ttccagattt gcctcgatct ctgccttgag atcactggtt
     2641 aaagttttca tagcgtcatc cgggctggaa acgcctctct cgattttttc taacgtttcg
     2701 gaaatcatgg tgtccattgt tcccatctcg ttgaagtgcc agtacgagta agcgaacgtg
     2761 agctgttcga aggccactcg ccacatcggt gtgctttcca tgaattgctg agtttcaggt
     2821 aattcgagcg ctgattttct cgttggaagg tagccggttt ccatggcgaa tttggctacg
     2881 ttctcgggtg tggtcaacca tttgatgagt ctgtaggtcg cttcgtcttc tttcgcattc
     2941 ttcgggaaga gaacggcaac agcgccgcct atggggacgg ccctaacggc tcccgccggc
     3001 aggaatgtgg ctccgaattc gaaactcgtc gtacccagcc atctgcctat tctgttggaa
     3061 ctgcccaggt agaaaccgag ggtacctccc agaaacatct tttctgccag agagtgctga
     3121 ccgacgggca tcgccttttc gtctatcaac attttccaga actgggccgt ttctataccg
     3181 cgcgcgttgt cgaagagcgg agtcatcgtg gccacatcga taatgtcgtt ttcgttctga
     3241 agcaataagg ctttgaatat ccacggggcg tcggccatgt cgactcccca gatgtccggt
     3301 acgccgtctc cgttagtatc tttggttata gccttcgcgg ctgtcaggag ctcttcccag
     3361 ttctgcggag ggttttcggg gtccagaccc gaggctttca ttaggtcctt gttgtaatag
     3421 aagatcggag tgcttatgtt gaaaggaatc gcgcagacct tgccctggta cttggagtag
     3481 tcccacatgg cggggtaaaa atcgtccata tcgaattcgg ggtcggccag gatgtagtcg
     3541 agcagaacgt aattatcctg tgcgccggtg aagatcggtc cggccgggat gatacctccg
     3601 ttgggcagcg ccttcgctgc gagggccgcc gttatcttct gtgcggtatc ggcgtagctt
     3661 cccgagtaaa ctgtcttgac tgtgatatcg ctctggctgg cattgaaagc atcgacaatg
     3721 gatttgatcg attctcccga tacgccgctc agggcgtacc agaaagtgat ctcggtaacg
     3781 gcgagcgata tagccgcaac cgagataacg attaaaagca gtactttttt cactatgaac
     3841 acccccttca ggatttgagt cctgtcatcg tgatcgattt tatgaattgc ttttcagaga
     3901 acatataaac cagtagaacc ggaactatgg ccataaccga tgcggccatc attagttgat
     3961 actcgctgct gccctcggtc atcaagtatc tcagcccgat tgggagagtt cgcatttcag
     4021 tagtgttgat aactatcagc ggccagagat actcggtcca gtgaccgaga aacgtgaaga
     4081 gagcgatcgt ggatattgcc ggcttcgtgt tcggcagaat aacgtgggaa aggatcccga
     4141 aatagccgca cccgtctatt ctggccgcat cttgcagatc gaccggaacg gttatgaaaa
     4201 actgcaccag aaagattatc gaaaaaccgt tgaagaggaa cgggactatc aggccctggt
     4261 atgtgtctat ccatttaagg ctgctgatta tcagaaaggt cggtatcatc gttacgacga
     4321 agggcatcag catggtaaca accacgaaca tgaagatgag attcttgaag ggaaacctta
     4381 gccttgccag cgagtaggcg gccagtatag aaacgataat ctgtccaagg gtggaaagcg
     4441 atgtcaccag tatgctgttg aaataatacc ggccgaaggg aaccatctcg aagactttcg
     4501 cgaaattgtc caggctccag gaactgggga accatcttgg aggataagaa tagacatcgg
     4561 cttttgtctt gaaggccgac aggaccatcc agaagagcgg gaaaagcatc gcgaagagtc
     4621 ctaccagcag cagtatgaaa gccactctgt ttcttacgcg ttcgcctttc aggcctttct
     4681 ttgtcattcg tagatcacct tcttgctgcc ggcttttctc tgtacccagg tgagcgagag
     4741 gatcatcagg aacagaacca ccgcagccgc cgaggccctg cctatcctgt aattctggaa
     4801 ggcctgctga taaatgtaga agaccatcac gccggtgctg tatagaggtc cgccggtcgg
     4861 tgtcatcacc gaaaccggag tgaatatttt gaaggagttt attataccca tgagtatgac
     4921 gaaataaatc accggcgaga gtaagggaag ggtgattctg aaaaaaatct tcctatcgct
     4981 ggcaccatcg attttggcag cctcggcgta agtttcgggg atcgcctgaa ggcctgccag
     5041 gaatattact atatcgtagc ccagaccttt ccacagggcg aaaaagatga ccgaaggcat
     5101 agcccactcg ggatcgtaca gccagcggat cggtttgact ccgaagagtc cgagtatctg
     5161 gttaagcggt ccgagcgatg ggtcgtaaat ccaaatccat atgagagagg ccgccaccat
     5221 tgggagaaca accggagcga agtatgaggc tctcagaaac ctgcgggcga agaccttcct
     5281 gtcgagaagc gtggcgatga aaagcgacac gaccagatcg agaggaatct ttaccgctgc
     5341 aaaataaagg gtgttcagaa tggatttcag gaacttctcg tctttgaata tgtactggta
     5401 attggagaat ccgatgaatt ttttggtcgc cctgagcaga tcccagttgg tgaagctgag
     5461 gtatatgaca ttcaccagag gtataacgac gaagacggtg gcgagaaaaa gcgccggcgc
     5521 cagacaaatg aaggcgaccg caacctccct ggggttgtac ctttggcgga tcttctttgg
     5581 catttttctc acatcccgaa aatgattttc atcgctctac ccctgttcaa tagctcgaaa
     5641 gcttcgcgcg catttctcat gtcgaacctg tgggttataa gtttgccgaa aaggtcgcgg
     5701 tttgcataaa gattaccgac ggccctctcg aagtgtctga acccaaaacc gaagctgcct
     5761 gttatactca cttccctgta atgaacggcg tacggatcga cgttgatagt tgaatccttt
     5821 ggatatccgc tgaagagcag gagagaacct ccgtctgcga ccagctgggt gtattccccg
     5881 actagctccg ggatgttgac ggccaggact acggagtcga aaaaaccgtc gggtcgctct
     5941 gcactcccct ctataccggc gcgacggata tactccagtc tccactcgga tttctctacg
     6001 atgcgggtct cgcaaccctc cgatagaccc acaagagcca ggagcatacc cataggacct
     6061 ccaccgacga taaggagttt ttccggtttt ctgccgagct tttcgaaacc gttgtagaca
     6121 caggccagcg gttcggccag agagtacagg ggatcgtcct cggctttgaa gaggcccttc
     6181 agagcgtgtt cttcgctcat cgatatgtat tcgctgaagc atcccgtctc aacgtaagtc
     6241 ttggacttgc acagttccgg aacacctctt ttgcacgaat cacacttgcc gcactccaga
     6301 taaggcgcaa ccgcaacgat gtctccgacc ttcaaaacgc cgcggtaatt gtttatcgca
     6361 tcgattcttc cgtagcattc atgaccgagt atggtcggag gtttgaacat ggggtgaccc
     6421 tttaggtaag tcttgagatc ggtaccgcat attcctgctc cgagaaccct tatgactatg
     6481 tccccagcgg gtttttcaat ttcccttaat tcgagttttc cgggtcccag ataaaatagc
     6541 gccttcatcg atcatcctcc agagttttca gaatatggct ggccgttttc tcatcggtga
     6601 tgagagtgtt gaaataacat gcgcgcgccg ccgcaattat cccttctacc ttttctaccc
     6661 ctccggaaag acacaccacg ttttccactt ctcttagttt accgatcggt attccgacga
     6721 ggttgtcttc cattccggat atgcagatct cgccgtcgct tttgaaaaac tgtcccagaa
     6781 tatcgccgac ggccccctgt tcgcggagta tcgccactat tgaaggctca atctccgcgc
     6841 tgagagttcc tttcggaccg ccgagaccta tgattgccag atcaagtttc gaccagtaat
     6901 cacccacgga atcgaaaggg tacgagagta tggtagaatg gtagaactct ccggcactgg
     6961 cgaaagccgg tatattcaga aagactcttt cagcaccgaa cttctcggcc agcctgtcca
     7021 ccatgctgtt gacctagtag aaaggaacag tctgccctat agcgccggtc aacggaacga
     7081 aaactatatc gcttcccctg gaaaagtggt ccatagttat tatcgttctg tacagggttc
     7141 tgccccaccc tacccctacg gttttggaat gcaatattat cgggggtaaa tacgagctgg
     7201 ccttcgccgc aatagattcg aagatcttct cctggtcgtc gtttttggac ctctccaggg
     7261 gcgccactac gacgtccttg agccccagaa gcgacttgat tctcgccgag atctcctcgc
     7321 tctcccctgt gtaaggatcg ttcacgttta tctccaccat accggtctcc cgggcgcgtt
     7381 tcaggtacct cgagatctgt ggcctcgaca ggccgatcct cttggctatc tgagcctgac
     7441 ttagatcgtt ttgataatac atcctggcta tcctgtacat caggagatcg tctatcgatt
     7501 tcgggatagc tttcaccccc tgataattcc gtcgagctcg gctatcagtt tcacggggtc
     7561 ctcgctctgg aaaatgttcc ttccaaacgc gaagcctttt acgccgcaat tgacggcgtt
     7621 tctggccatt gttagtacat cgaggtttct gggtcctccg gccagtatta ccggcaccgg
     7681 acagccctga accaccacgg agaaatcttc cgtatagaag accttcaaaa catctgcgcc
     7741 gagttcggcg gctattctcg ctccagtctt gaccagatcg gggtcgctgg tcttattgaa
     7801 gtcttcggcc agtacctcca cgaccacggg gatcgatagc gagtgatatt cgtctatcgc
     7861 gcgggcgacg ttctccatcg agtcgtagtc gcgacctccg agaacgagca taagtatcgc
     7921 cgcatcggcc ccgagatcga gaagggattc ggcagagacg accgcaggat ggaaagtctt
     7981 gtagtcggag aacttgctgc cgccgatcga cgatctcacg atcaacttct tgcccgacac
     8041 ggtcctggca ttcaaaagtc ttgttatgcc ggggtttacg atgaatccat cagctctgga
     8101 catcgagagt ctctcgatga gttcagccgg tttatctatc ccttcgacgg gtccaaagaa
     8161 ctgcggatga tctagtgcga caacaaagag gtgccctccg ttagaaaata ggcttgcaac
     8221 atttctctcg agtccggtca agtcgatcac gtccttatga aaaatatacg cacatttgtg
     8281 cataataaat gttcattatt attatatcaa aaacgatatg ggtaaatcaa tcaaatcaaa
     8341 aggctttctc atcgattaac tcatggagaa aaagcgggcc cacagcgggg cccgctcgat
     8401 ttgaattacc agtctctata aaccttcgga tcctgtataa acccggctat ggttctttcg
     8461 gtgagtgtta gcgaagtggt ctggggagtc gctcctttta tggccgtgaa gcacttgtag
     8521 aggagggccg cgatgtcgga agtggagtat gtcgtggcgt aggtctgaac ctctggccag
     8581 tgtgcgtcgc tagtgaagta cgccattttc tggaggaagt ctcccgacca ctccacgtaa
     8641 acgtcggaag ggttgaagtc ggcgagcacg ccgccaacat gatcccagtc gggataagca
     8701 ctcttggacg aactcgttgt ggtgatggct atctcgatgt tggccggcat cacaccgccg
     8761 gatttaagtc catcgagcca gcttccggcg tggcaggatt cgatgatcag acagaaagtc
     8821 gtgttggcat gttcctgtat atagcttctg agttgagagg catagaaagt ggttccgccg
     8881 aggttcatca ggttgatgtt gccgtgggct acgaaataga gcgtcatgtg gtttatcttc
     8941 tcgttcgtga agagatcgtt catcgcgttt tgaacggccg tccagttggg ggccggtcca
     9001 ccggcagtgt acttcacatc gcgcacttta tcggctccca taagtgtttt gaaggaagtc
     9061 gacatgagat ctctggcgtc cctcgcctcg gcgtagagat tctgggtcgg agtaagaccg
     9121 ctggttatta cagccccttt acggattatt ctggctatta tgacatctat ccagtctata
     9181 attggaagat aaacgatttt actcttgtcc cagatagtgc ttgctatata ctgtgaacgc
     9241 gtgagtaagg gagcgggtag ttcgtcgttc accttgggca cgccttcgat cttctgatcg
     9301 aagattttct gtccgttttt gctgattact accaatcttc cgggatggtc gtagaaagct
     9361 cccggagcct cgtcgagata gaaaagccat ccggcctggt tcagaccctg aagctggtat
     9421 tgaaattcct gcccgccgtc ggccgtttct gaagtgatga tatctcctac ggcgacctcg
     9481 gccaggcgaa ctccgacaaa ggtatcttcc ggctctccct ctttttttat cagttcaatg
     9541 aacttctttg tggcggcctc taggggatct ttgggaaaac aggcagacag caagaaaaag
     9601 acagttgcaa gaactatcat tacaagaact actcgtttca gccgcatagt atcaccttcc
     9661 ccgaatgcgg agcagtgtat tgcctacacg gaattctatg taaatatgtc atcttgacag
     9721 acaattatac aatcatgaag gtcttgaaag acacatagtt atacacagtg taatctctct
     9781 ggaaaagctg ccaaaagcca ttgttcaaga ggtgttttac gatcgagaac ccgagattgc
     9841 agtacaggtt atgggtaagg atgaacaaaa aagagggcgt ttccgccctc ttcttgttca
     9901 atcgttatca gtaatactcg atttccctgg tttcttcgct ttcgacggtt gccagttcct
     9961 tccagttttc cttctcgaga tcctgaatac tgtaaaggaa atataccgtc atgtgagtgt
    10021 agttgcccgc cgcatcgtat tcgtactcat agaccgtaac ttctttatcg tcactgaaga
    10081 agctatcgct tatcttcttg aatacgtctc cccattcgtt gtactcgaac tgagacgcaa
    10141 taggctccgg ttcggtgccg ggttcggcga acatgtacat gtactcgagt tctcttatct
    10201 tgagattgtt gctgtcgtat tcaaaaaccg ttttcgatag taccattccc atcatgctca
    10261 tggactcttc tattaggttg cctctttcat cgtaggccat gtttacggcg atctgtattt
    10321 cctgcccttc gctcattcca gatatgatct gggcgacgag tctgccgttt tcgtcgtact
    10381 ccatgttgat ggtggacttg tcttcgcctt catctgcaac gatagaaatg attttaccgt
    10441 cttcgatagt caattcccct attatctgtt cctcgccgtt ttctcttcca gagatcgcgg
    10501 aaagctttcc gaaagcatcg tagccgtaaa ggatggcatt tatctctttt ccgtccttat
    10561 cgtaagagat ctgagaaact atctttcccg cttcatcgaa agagattacc tcgtacgcag
    10621 gcatcactac atcttctccg agatcgacat cgcttggcgg gtagactgtc ttcatcgatt
    10681 tcaccggacc tttgagggcg aaatcggccg tactcagggg aatcatgaac tccgactgtg
    10741 cgaacaaaac cattgccaac aggataattg tgcttactgt gagaagtttc ttcacttctt
    10801 cacctccact gaatgagtta ttattatcat acatgacctt gatcaaagat gttatccata
    10861 atcactgtct cgcgggagaa agatgtatta tatcgatgaa gcgacgcccc aggaactgaa
    10921 cgaaataaga acgagaatgt acaaagccat accggaatcc agcaacggta gatattcgcc
    10981 cggggaattg aggccggtcg atgaagttta ccgtgacttt ctcaatgccg gggatagcgt
    11041 ggccgtcatg agatgccggg gaacgatcgt aggatacatt catttcggcc gtagaaggga
    11101 agagacttat tcgccggaag aaaccggaca tatcttcgac atcttcgtcg aggaggctca
    11161 tagaaagaga ggaatcgcca cgcagctcct ggatcacgcc ctttcccgga tgtactctca
    11221 gggatacggg aaagtatccg gcaacacttt tcggggcgga ccgggctggg agatccttct
    11281 aaagagaggt ttccgtccag tcactgtttg cctctcgatc gaaagccgat ctcccgtccg
    11341 cgaggagtct ggcgtatctc taggtgttcc ggccgacctt ttgatgttct cggaaggcct
    11401 aacgatttct cgcgccgagc tggcgcaaga tctttcgatg aatctcagtg aactttatta
    11461 taggctcaac gccgtcattg aaagaggggg gaccttgctt atcgattcgc acggtggcag
    11521 tccggcgggg tttctggttt actctgtttt tgaggacctg atgacttacg actttctcgg
    11581 ctacctggat ttcgtatttg tagttcaccc cttcagaagg ctgggggtgg ccgggagtct
    11641 cctctatgaa tctctgcgca ggctgaaagg ggttcaggcc agggagatgt ttttcgaatg
    11701 caactgcgac gcatattgtt ataaatgggc cctctcggtg ggcatgaaag agttttccat
    11761 attgctcgag aaagatctgg atcgcctttg atgaaaggta cttacgggaa ctttccgagg
    11821 ggcgacaaaa agagtcttta tcgattaaaa gcgttgttgc gtagcaataa cagtcgatat
    11881 agatgataga ctttatgaaa gagataaata tacccccagg gaggtcggag agatggagct
    11941 gctcgagaga atcaaaaacg ccataatcga aacgatgccg gtgatggcga agcaggcgac
    12001 gcaggaggcc cttgaaaaag gcctaagtgc taaggatata ctgaattccg cattgattcc
    12061 gggcatggac gaggtaggaa ggttgttcag aggaggggaa tacttcgtac cggaagttct
    12121 cgttgcggct aaagccatga acgcctcgat ggatcttata gaaccgcttc tggtcggtag
    12181 cggggtcagg aaggtaggca aggttgtggc cgggactgtc gagggcgatt tgcacgatat
    12241 aggcaagaac ctggtttgta tgatgctcaa aggggcggga ttcgagataa tcgatctcgg
    12301 tgtcgacgtg aaaccggaga gtttcgtcga ggccacaaaa cgagaaaaac ccgatatact
    12361 ggttatatcg gccttactta ccacaacgat gctcaacatg ccgaaggtca taaacgcact
    12421 cagagaagcc ggcgtgagag aaaaggtcaa ggtgctcata ggaggcgccc ccgtgagccg
    12481 tgattatgcc gaggaaatag gggctgacgg atactccgaa gacagttctt cggccgtgga
    12541 actggccaga aagctggtaa ctgtctgagg tattttgtgg tgagacacgc aagttccttc
    12601 aaatgcaagg taattgattt ataattcgaa tagagtctat atttttatac gcacgattta
    12661 tatactaacg taaaatccag ccggatctca acgacactcc ataaatgggg gaaagtcatg
    12721 tacatacgta tcttttccat ctggcctgtg acctcaccgg tattatgttg gactgtccgg
    12781 atcgaccgat aacatggaga agaattattc aagcctttac aacgccctcg tagatcatcc
    12841 cgatctgctg atctgccggt ggctgcccga tacttcgctc acattcgtca acgatactta
    12901 cgcccgtttc tacggatacg agaaaagcga aatgataggc aagaagtggc tggagatatc
    12961 taagatcgat atccaggagg atttgagact ctcggtaaac cgggccgaag cgttgagaga
    13021 cagtatagtc cgcgaggatc cggcgatcga tccggaggga aaggaacact gggttcgatg
    13081 gtacaacagg ccgatcatag acgattctgg cacagtggtg gagttccagt cgatcggaat
    13141 agatatcacc gatcagaggc agatcaccca gaaattgctg gaatccgagc gtgaaatgtc
    13201 gctcgttctg gacaacatcg aggaactggt tgcctatcac agacccaacc tgagccttgt
    13261 gtggatgaac agggcctacg ctagatcctg cgatagctca cagagagatc ttacgggaag
    13321 gttttgtcat gagatctggt atgggtcgaa acagccctgt gaaaactgtt cggtggctct
    13381 ggccgtgagg gagaggaaaa atgtaacggg cgaaatcacc agcggtgaaa gagtctggca
    13441 tataaaggcc tacccggttt tcggtcaaga cggcgaactt ctgggagtgg tcgacttttc
    13501 atatgaagtc accgaagaga ggaaactctc cgaagaactc atcaggagtg aggagcgttt
    13561 caaaagactc gttaattcga ccgacgatat aatcttcgag ctcgatagag aggggagaca
    13621 caccgcaatt catggccgct ggctcgagaa aatgggattc gcagaagagt tcttccttgg
    13681 aagatcggcc ggagacatct tctcggctga ggatgcgctg gttcacgagc agaacaacgc
    13741 gatcgctctc ggaggcaaat cgacgaaata cgaatggtct accgaagtag atggaataca
    13801 tatcttctac aatacttctc tctcgcctat cttcgaccgc gaagggaacg taacgggaat
    13861 agtcggcatc gggagagata taaccgatat aaaaaggacc gaagagaccc tcaagagaag
    13921 ctgggatcag ttgataatga ccttatcgcg gatgctgagg ataaaagatc cgtacacggc
    13981 cggtcatcag g
//
LOCUS       DAOOLY010000030        13958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4751_length_13958_cov_5.214990, whole genome shotgun sequence.
ACCESSION   DAOOLY010000030 DAOOLY010000000
VERSION     DAOOLY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13958
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4751_length_13958_cov_5.214990"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1374
                     /locus_tag="PLD15_02750"
     CDS             76..1374
                     /locus_tag="PLD15_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB62847.1"
                     /translation="MGEKKKYYIISDLHIGGDGELEDVTFKSELISFLNSLSETDRSD
                     EVVELIIAGDLFGFWELSTVEGPGQLSFVLDRYADVFEAFRKAGESVRITYIVGNHDH
                     TVLGYPGSKEKLGEYNIFLDENQHLIREIAGEKIWIEHGHQHDKFNEIDEPGSELCRP
                     AGFYITRYLLSLAGLISRMGRKNWLVNVRCVQPLNALPRWMFSSYFYLEMHPILRLAI
                     VPFLFFFAVSIVLFVMGILDIFGLNTVQYLYEDTLKSFGVVGDVVDTIIGIDVLMLII
                     FVVVSIPLFIVFRDIKSALKRYGLVYEKKEKAEKEQAYLEGARKVFENDGSIRFFIFG
                     HTHNPFLLKDGDRYVINTGTWLERLERVKSFIWPFPSIFYPRYDLNYFVIAHDGKDTV
                     VEYHRIDKAPSTKLTLLQRFLISLRKKPSTIGIPSRTKIQ"
     gene            1463..2098
                     /locus_tag="PLD15_02755"
     CDS             1463..2098
                     /locus_tag="PLD15_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB62848.1"
                     /translation="MKKSAATARVIVEILFLALFVFLLRERSLQKWFLIFGGGLVASL
                     VFGRIYCGWICPMETLFRPIDWLYSKLGIKRFKTPKLFRGSAFRWIVLALFLLLMVAT
                     RLFHLKVNLLLYITALSVLVTLFFEEEFWHRWICPFGTLLSLFSKRPLFGMRVKKPTC
                     VSCGICQRVCPVDAIEREEDGMKIDTSECLVCLKCGEKCPKLSINYSRSDR"
     gene            2230..5388
                     /locus_tag="PLD15_02760"
     CDS             2230..5388
                     /locus_tag="PLD15_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB62849.1"
                     /translation="MDRSYQDNSINASQALIERIKELNCLYTLSQLLADSRTVEEAVR
                     AVLQTLPPAFQFPDSAISRVLIDGRVYSSREEWFPDRSIRCKLTIEGREAGCAEVSYS
                     PAGSGRSLEFLPEEEKLLATVARDLGSTIERVEFFNELTESKERLSFAIEASREGVWD
                     WNVETGEVYFSPRWKEMLGYSDSEIENRLDEWESRVFPGDLEKVERELQRCLNGEIPY
                     YEAEHRVMTKDGSYKWILDRGKVVLRDAQGRALRMVGTHTDLTEHHRIEEELRRYNRA
                     LSFLSEVNQFLVRVTDEEELLKGTCDIATKFGGYALAWIAYKVPSRSDLKPVVSSGLA
                     STYPGKISVTFDESELGRSAVGTAVRTGTTIIKSSIDKSLEYSPWKNMIDSYGLKSAI
                     SLPLKIEGETIGALNIYSSDENAFDQEEVHLLEELSGDLSYGILSLRNRKKLEHLNRV
                     LLSIRSINQLIVREEDRGTLIRKITDLLAGLRSYDKSLLVILDENMKVVDWSSSGFEG
                     AKLEKVETALRENLLPPFLTRVLTSEEVLMIPNEEYRRSNLSGDDQTGHAVLLKSLKS
                     SDRISGFIAAIMPENFSQDLEERSLFGEIANDIALAISKIELKNERESLQERFALFMD
                     KFPGAVFMQDRDLRLSYFNKYMEKYFGIDSHWLGKSADQFLSGEWGRSMLGDARKVRP
                     GESISEELTARCTDGRDRIFFVQKFPISSGSDTSMIGGIALDITDRKLSETMATRLGM
                     VLDWSVNEIFVFDHETLRFLLANRGAIENTGYSMDELKEMTPLDLKKETSPAEFKKII
                     DYLKKGENRSVVFETVHTRKNGSKYPVEVRLQLHDDNMGKVFVAVIVDITERKKAEDS
                     VRRSLESLIKTLSVLVESRDPYTYGHQKRVSELATAIAARLFADDTQGKDKIESVRVA
                     ALLHDIGKNAVPTEILTKPIQLNKIEFELVREHARQGYEILKDVYFPWPIAEIVRQHH
                     ERLDGSGYPDKLTGDSICLEARIIAVADVVEAMSSHRPYRAALGIDAALEEIRKNAGR
                     LYDTTIVKACLEVFKEGFEFSD"
     gene            complement(5378..6307)
                     /locus_tag="PLD15_02765"
     CDS             complement(5378..6307)
                     /locus_tag="PLD15_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62850.1"
                     /translation="MDGHDALTYYCLDLESDYANERVEITPYSYASVDPGPYNPARSA
                     FEDYLGEKYLVDSDHELYRPFFDNCKPVDGRAPVWQILTVYSYEPDFGMDGKMALSPL
                     QRLMGSSQAWRHEEFHMGLRFGRVTEMVEYFDRLSRIAFERGDRYWGFRFMARTVHYL
                     QDSGTPYHTSPGTTGDILMIPLAYRKQFRKISNYHKFHDRYTGYRLWRGYGPFLEAIR
                     LAALSHGLSDTGKLAVEARRRGLRLVKDLQSSIRTVAGEGFDTLDGFRGDRAYFDEVS
                     RERETKELDIITARFLSGMAGLVKSYLKTAFSR"
     gene            complement(6309..7388)
                     /locus_tag="PLD15_02770"
     CDS             complement(6309..7388)
                     /locus_tag="PLD15_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB62851.1"
                     /translation="MSLILKNVRKVFTSYSVETVAVDNFDQVIEKGHLVTLLGPSGCG
                     KTTTLRIIAGFEIPTSGRVLLEERDVTNLPPNKRNISMVFQSYALFPHLTVEENIAFG
                     LKLKRLDRSEMRRKIVEMTDLVGLKGLEKRRPDQLSGGQQQRVALARSLVMEPSVLLF
                     DEPLSNLDAKLRETMRLEIRRIQQEVNITSVYVTHDQVEAMSISDVIVVMNQGKVMQI
                     GSPFDIYARPENSFVADFIGRVNFIDGTVESVDGDTVVVSAGATGNKIIGKRGTELVP
                     GKPARVVLRPESLSDRKEERINSLSGKVLKYVFLGSNVEYELELSDGRKINAVTYNPV
                     ERKLPVVERETELYFSRESAWVIRV"
     gene            complement(7393..9087)
                     /locus_tag="PLD15_02775"
     CDS             complement(7393..9087)
                     /locus_tag="PLD15_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB62852.1"
                     /translation="MAFRSEETRNRLLNLLKDPVLLLTIIIIFAALAIFIVYPIFKVF
                     SASLIDKNGQFDLSAYTKAFSNRYMRQGFFNSLLVAGLTAFFGMLIGYIFAYTINRTD
                     IPLKGFFRTVAVLPIVFPPFIGALSIIMLFGFNGFITAGIFGIRNFPVYGLWGLMMAQ
                     VVCFFPVAFITLDGVIGTISPTLEDAAFNLGANRWQAFRKVILPMSIPGIASTMLVLF
                     IESLADFGNPLILAGSKFPVLSVQAYLQITGMYDTRGGAALAVWLLFPSIVAFIIQKY
                     WVGKKKYVTVTGKPTTSVLKSVNRPVKWLLFSLCMLMAAFTLAIYATIFWGAFVKAWG
                     MNNTLTLENFRYVFDVGLEAIKDTLIIAFTSTPISALLGMVIAFLLVRKIFPGRRLME
                     FTSLLSFAVPGTVIGIGYILAFNTKPFLLTGTLAILVLNFVFRYVPVGIQSGVALLNQ
                     VDPAIEEAAYTLGADNRQVFTRVTLPLIVPAFFSALVFAFVRAMTAISAAIFLVSARW
                     NLITVQILSQSDSGRLSEACAFSVILIAMIMAFILVLKIFLKNKVSLSGSESMGQG"
     gene            complement(9229..10209)
                     /locus_tag="PLD15_02780"
     CDS             complement(9229..10209)
                     /locus_tag="PLD15_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62853.1"
                     /translation="MKRVLILSLFLMLVAVTLANLVVYSSVDEANARKILNAFVEETG
                     VGVDFVFLSSGPALARIEAEVNNPQADVWFGAPLENHIIAKERGLTQPYKTLSVYGVS
                     PEFYDVEGYYHPIYMNPLGIGINTAVLEQIKASMPVTWEDILSTSLSRMIQYPNPQSS
                     GTAYSLITGLIQIYGEDGAIDYLKKLAPNIQTYTQSGTGPSKAVGPGQVAMGIQFTPA
                     FFQFMEQGYPVKVVFPKEGVPYEVASVSILKGARNLTDAQKLIDWVVSKAGQQQIVNQ
                     QTYFYPIRSDVDFGSLQPLSTIKLLPVDPVWAAENKTRLIERWIDEVLPY"
     gene            10511..11374
                     /locus_tag="PLD15_02785"
     CDS             10511..11374
                     /locus_tag="PLD15_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB62854.1"
                     /translation="MVSKKISEMMGQFSPVNRKIASCVLRNYPEMGFISIESLSSMAG
                     VSKASIVRFSRAIGFSGFNELKKRIQSDLKKKLQPYEKIAATDLDKKNVDEQLRELAR
                     NEIDNLRKTLSSIEEGELLKWIGAIGKARNIYFAGFGATRHIVSLMQYTFSVLQRKPT
                     WLLDGSISDFSYKIKLMDKSDILILMTFPPYSKEIEYIVRFARERDVEVFLITDSVEC
                     PVYSQARSTIVCENNSLLFGNSFVGPIALIEVIGNFLILGEKELGMTEMKTLWEVEEK
                     GYSLIDSQFEK"
     gene            11568..12473
                     /locus_tag="PLD15_02790"
     CDS             11568..12473
                     /locus_tag="PLD15_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HPB62855.1"
                     /translation="MKAIVVHGGAGSFESEEDHRNHKRGIAEALEAGFSMLDRRKSSI
                     EGVVAAVKKMEEDPAFNCGKGSVLNLKGEVEMDAAIMDSELNAGAVSGLKRILHPIEV
                     ARAVMEQTDNVLLSGQEVEEFIRIMGFPREENLVVPTRQMQWQRNMEKIERGENLRFS
                     RTIALARKTPGYYSTSGAVAIDDNGKMAAATSTGGIVMKTFGRVGDTPVIGAGTYANN
                     FGAVSATGHGEKIIKLTLCRLVAFFMEQYPAQKAVDLALERAKYIDCECGLIAIDRYA
                     NIGIGYTTRDMSWAYAREGHKPVTF"
     gene            complement(12481..13863)
                     /locus_tag="PLD15_02795"
     CDS             complement(12481..13863)
                     /locus_tag="PLD15_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB62856.1"
                     /translation="MKFSSSEERARALGKMKIPSLLIGLSIPSIIAMLTNAIYNLVDA
                     FFIGRIGTSAMGAIAIAFPIFNLIGAVGLTYGVGGASYVSRLLGAGDREQADRAASTA
                     FFTSLATGIVFTILGLTFLDPLLRAFGATETIMEYAREYTMVIIAGSILNMMNMTMNN
                     LVRAEGNAPRFMMAMVSGALLNVALDPIFIFGFGMGVVGAALATVISQSVSTMILLEY
                     YLRKKSYTRLSIKLYRFSLHIYGEIFKIGLPTFLRQFLSSFSVALLNNAAAAYGDHAV
                     ASVGITMRVLMLGMMVLFGYGQAFQPVTGYNYGARNYSRIFEALKFSLMVTTAFAIVF
                     GIVGMVFPGMIINIFSDDPEVIEIGSQALRAVSIFFPTFGFVLTFNYLFQGMGKGLSA
                     GILSMAKQGIFLIPAVITLPRIFGLNGVIYAQTTADFFTLFVAGILAMGAIKKLRVES
                     RSQIADGRAE"
BASE COUNT         3481 a         3570 c         3786 g         3121 t
ORIGIN      
        1 aaactcggac gcgaaaaaat tcacaggtgt gataaagtat aagggtagcc gattttcaag
       61 accgcggggc gtcgaatggg agagaaaaag aagtactaca tcatcagcga tctgcacata
      121 ggcggagacg gggaactgga ggatgtaacc ttcaaatccg agctgataag ttttttgaac
      181 tcgctgagcg agaccgatcg ctccgatgaa gtcgtcgaac tgatcatcgc cggcgatctc
      241 ttcggtttct gggagctctc caccgttgaa ggacccggtc aactttcctt cgttctcgac
      301 aggtacgccg atgtcttcga ggccttcagg aaggccgggg aatccgtcag gataacctac
      361 atagtgggaa accacgatca cactgtgctt ggctatccag gttcgaaaga gaagctaggc
      421 gaatacaaca tctttctgga tgaaaatcaa cacctgataa gagaaatcgc cggcgagaag
      481 atctggatag aacacggcca ccagcacgac aagttcaacg aaatcgacga acccggttcc
      541 gaactttgca gaccggccgg cttctacata acgagatatc tcctgtcgct cgccggcctc
      601 atatccagaa tgggcagaaa gaactggctg gttaacgttc gctgtgtgca gcctttgaac
      661 gcgttgccga gatggatgtt ttcgagttac ttctatctgg agatgcatcc catattgagg
      721 ctggcgatcg tgccctttct cttcttcttc gcggtcagta tagtcctctt tgttatggga
      781 atcctagata tcttcgggct caacaccgta cagtatctgt acgaagacac gctcaaatcc
      841 ttcggcgtcg tgggggacgt ggtcgatact atcatcggaa tagatgtttt gatgctgatc
      901 atattcgttg tggtttcgat cccgctgttc atagttttca gggacataaa gtcggcactc
      961 aagcggtacg gtctcgttta cgagaagaag gagaaggctg aaaaagagca ggcctacctc
     1021 gaaggcgcga gaaaggtctt cgaaaacgac ggatcgataa ggttcttcat cttcggccac
     1081 acgcacaatc cctttctcct gaaagacggc gatcgttacg ttatcaacac gggcacctgg
     1141 ctcgagaggc tggaaagagt gaaatctttc atatggcctt tcccgtcgat cttttacccc
     1201 cggtacgacc tgaactactt cgtaatagct cacgacggca aagacaccgt cgtcgaatac
     1261 cacaggatcg acaaggcgcc ttccaccaaa ctcacgctcc tccagaggtt tttgatatcg
     1321 ttgagaaaaa agccctcgac catcggaatc ccctcgagaa cgaaaattca atagacgaaa
     1381 taatcttcat ctaagtgtga cacatatcac ataccggaag ctcccttcga gatagactgt
     1441 acttgaaggg ggagatctta tgatgaagaa aagcgctgca accgcgagag tgatagtgga
     1501 gatactgttt ctggccctgt tcgtttttct gctaagagag aggagccttc agaagtggtt
     1561 cctgatattc ggaggcggac tggtcgcatc gctggttttc gggcgaatct actgcggctg
     1621 gatctgtccc atggagacac tattcagacc aatcgactgg ctttactcaa agctcggaat
     1681 caaacgcttc aagacaccca aactcttcag gggaagcgcc ttcaggtgga ttgttctcgc
     1741 tctgttcctc ttgctcatgg tcgccaccag actcttccat ctgaaagtta acctgcttct
     1801 ctacataacg gcgctgtcgg tactggttac tctcttcttt gaagaggagt tctggcacag
     1861 atggatctgt cccttcggta cgctcctgtc gctcttttca aagcgaccgt tgttcgggat
     1921 gcgcgtcaaa aaaccgacct gcgtctcctg cggcatctgt cagcgtgtct gccccgtcga
     1981 tgccatagaa agggaagagg atggaatgaa gatcgacacg tcggagtgtc tggtctgcct
     2041 gaaatgcggc gagaagtgcc cgaagctttc aataaactat tcaagatcag accggtaaga
     2101 aacggtgaaa ggctcttccg atgacttgac aatcacaatt cccaccggga aatatggttt
     2161 catctttgca ataaatcgag ggaaaaatgg tacgattgcc atatagttcg tctttcggga
     2221 gaggataact tggaccgcag ttatcaggac aattcaataa acgcgtcgca ggctcttata
     2281 gagaggataa aggagctgaa ctgtctttat actctctccc agcttctcgc cgattccaga
     2341 actgtagaag aggccgtcag ggccgttcta caaactcttc ccccggcttt tcagtttccc
     2401 gacagtgcga tttcacgggt actaatagac ggtcgggttt attcttcgag agaggaatgg
     2461 ttccccgaca gatctatccg gtgcaaactg acgatcgaag gtcgtgaagc cggttgcgca
     2521 gaggtttctt attctcccgc cggaagcggc cggtctctgg agtttctgcc cgaggaggag
     2581 aagcttctcg cgacggtcgc gcgagatctt ggaagcacga tagaacgagt ggagtttttc
     2641 aacgaactca cagagagcaa agaaaggctg agtttcgcga tagaggcgag cagggaagga
     2701 gtctgggact ggaacgtcga gacgggagag gtttacttct cgccaagatg gaaagagatg
     2761 ctgggctatt ccgactctga gatagagaac aggttagatg agtgggaatc gagagtcttt
     2821 ccgggcgacc tggaaaaagt cgaaagagaa ctgcagagat gtctgaacgg cgagatcccc
     2881 tattacgagg ccgagcacag agtcatgacc aaagacggct cttacaaatg gattctcgac
     2941 cgcggtaagg tggttcttcg cgatgctcag ggcagagcct tgaggatggt gggaactcac
     3001 accgacctca ccgaacacca caggatcgag gaagagctga ggcggtacaa cagagccctt
     3061 tccttcctga gtgaggtcaa ccagttcctg gtacgtgtga ccgacgagga ggagcttctc
     3121 aaaggaacgt gcgacatcgc cacgaagttt ggcggttatg ccctcgcctg gatagcctac
     3181 aaagttccct ccagaagcga cttgaagccg gttgtctcga gtggtctggc aagcacgtat
     3241 ccagggaaga tcagcgtcac cttcgacgaa agcgagctgg gaaggagcgc tgtcggcacg
     3301 gcggtgagga ccggcacgac cattataaag agctcgatcg ataaaagcct ggaatactcg
     3361 ccctggaaaa atatgatcga cagttacggg ctcaagtcgg cgatatcgct tcccttgaag
     3421 atagaagggg agaccatcgg cgccctcaac atatactcct ccgatgaaaa cgccttcgat
     3481 caagaggaag ttcatcttct ggaagagctt tcaggagatc tctcctacgg gattctctcg
     3541 ctgcggaaca ggaagaagct ggagcacctg aacagagttc ttctatctat aaggagcata
     3601 aaccagctta tagtgagaga agaggacagg ggtacactca tcaggaagat aaccgacctc
     3661 ctcgccggac tcaggagtta cgacaaatct ctcctcgtta ttctggatga gaacatgaag
     3721 gtcgtcgact ggtccagttc gggattcgaa ggagcgaagc tcgagaaggt agagacggcc
     3781 ttgagagaga acctgcttcc gcccttctta acccgggttt taaccagcga agaggttttg
     3841 atgatcccga acgaggagta tcgcagatcc aacctctccg gtgacgacca gaccggacat
     3901 gcggttctat tgaagagttt gaagagtagc gatagaattt ccggcttcat cgccgccatc
     3961 atgccggaga acttttcaca ggacctcgaa gagaggtcgc tctttggaga gatagccaac
     4021 gacatagccc ttgcaataag caaaatagag ctgaaaaacg agagagagag tctccaggag
     4081 agattcgcgc tcttcatgga caaattcccc ggagccgtct tcatgcagga cagagatctc
     4141 aggctcagct atttcaacaa gtatatggag aagtatttcg gtatcgacag ccactggctg
     4201 ggaaagagcg cggatcagtt cctctcaggt gaatggggca gatccatgct gggggatgcc
     4261 agaaaagtcc ggcccggcga gagtatttcc gaagagttaa cggccagatg taccgacggc
     4321 agggacagga tcttcttcgt gcagaagttc ccgatctcct ccgggagcga cacttctatg
     4381 atcggcggca tcgctctgga tataaccgac agaaaactct ccgaaactat ggcaacccga
     4441 cttggaatgg tactggactg gtcggtgaac gagatcttcg ttttcgatca cgaaacgctc
     4501 cgttttctgc tggcaaaccg tggcgcgata gaaaacacag gctattcgat ggatgaattg
     4561 aaggagatga caccgctcga tctcaaaaaa gagacctcgc ccgccgagtt caaaaagatc
     4621 atcgattacc tgaaaaaagg tgagaaccgc tcggtggtct tcgaaaccgt tcacacgaga
     4681 aagaacggtt cgaagtatcc cgtcgaggta cgtttgcaac ttcatgacga caacatggga
     4741 aaagtcttcg tggccgtgat agtcgatata actgaaagga aaaaggcaga agattctgtc
     4801 aggagaagtc tcgagtctct cataaagact ctctcggttc tggtcgaatc tagggatccc
     4861 tacacctacg gtcaccagaa gcgggtgagc gaactggcta ccgctatcgc tgccagattg
     4921 ttcgcagatg atacccaagg aaaggacaaa atcgagtcgg tgagggtggc ggcgctgctg
     4981 cacgatatag ggaagaacgc cgtgccgacc gagatactca ccaagccgat tcagttgaac
     5041 aagatcgaat tcgaactggt gagggagcat gccagacagg gatacgaaat cctgaaagat
     5101 gtttactttc cctggccgat cgcagaaata gtcaggcaac accacgagag gctggacggc
     5161 tcgggttatc ccgataagct caccggcgac tcgatatgcc tggaggccag gataatcgcc
     5221 gtggccgatg tcgtggaagc gatgtcttcc cacaggccgt acagggcagc gctcggaatc
     5281 gatgcggctc tggaggaaat aagaaaaaac gcagggcggc tttacgatac taccatcgta
     5341 aaggcctgcc tggaggtctt caaagagggc ttcgagttca gcgactgaag gccgtcttca
     5401 ggtacgattt caccagcccg gccatccccg acaggaaacg cgccgttata atgtccagct
     5461 ctttcgtctc tctctctcga gagacttcgt cgaagtaagc cctgtcaccc ctaaatccgt
     5521 cgagagtgtc gaatccctct ccagcgaccg tccttatgct ggactgaaga tctttcacca
     5581 gcctcaggcc gcgtcttctg gcctctacgg ccaactttcc cgtgtcgctg aggccatgtg
     5641 agagagccgc caatcggatg gcctccagaa acggcccgta cccacgccag agccggtaac
     5701 ccgtgtacct gtcgtggaat ttgtggtagt tgcttatctt tctgaactgt tttctatagg
     5761 cgagcgggat catgaggata tctcccgtgg ttccggggga ggtgtggtat ggcgtaccgc
     5821 tgtcctggag gtaatgcacg gtacgggcca tgaacctgaa gccccagtag cgatcgcccc
     5881 tttcgaaggc gatccggctt aatctgtcga agtattcgac catttccgtt acccttccaa
     5941 agcgcagccc catgtggaac tcttcgtggc gccaggcctg gcttgaaccc atgagtcttt
     6001 gcagtggcga gagcgccatt tttccatcca ttccgaaatc cggctcgtaa gagtagaccg
     6061 tgagaatctg ccacaccggc gcccgcccgt cgacaggttt gcaattgtcg aagaagggac
     6121 gataaagttc atgatcggaa tcgaccaggt acttctcgcc gagataatcc tcgaaggcgc
     6181 ttcgggcggg gttgtacggt ccgggatcga cggaggcgta ggagtaggga gttatttcta
     6241 ccctctcgtt ggcgtagtcg ctctccagat cgagacaata ataagtcagc gcgtcgtgac
     6301 cgtccatttc aaactcttat cacccaggcg ctctccctgg agaagtagag ttccgtctcc
     6361 ctttccacga ccggcagctt ccgctcgaca ggattgtagg taacggcgtt tatcttcctg
     6421 ccgtcggaca gctccagttc gtattcgacg ttcgagccga gaaagacgta cttcaagacc
     6481 tttccgctca gggaattgat ccgctcttcc tttctgtccg acagcgactc gggccgtaga
     6541 accacccttg ccggcttgcc tggcacaagc tcggttcctc tctttccgat tatcttgttg
     6601 ccggtcgctc ctgccgaaac gactacggta tcgccatcga cagattccac agtcccgtcg
     6661 atgaagttga cccggcctat gaaatcggcg acgaagctgt tttcgggacg ggcgtatata
     6721 tcgaacggac tgccgatctg catcaccttt ccctggttca tgacaactat cacgtccgat
     6781 atgctcatcg cctcgacctg gtcgtgcgtg acatagacgc tcgtgatatt cacttcctgc
     6841 tgaatacgcc ttatttccag cctcatcgtc tccctcagct tcgcgtcgag gttcgaaaga
     6901 ggctcgtcga agagcaaaac actgggctcc atgaccaggc tccgggccag agccaccctc
     6961 tgttgctgac cgccggacag ttgatccggc ctcctttttt cgagaccctt cagccccacc
     7021 agatcggtca tctcgacaat ctttctcctc atctcggagc ggtccagcct tttcagtttc
     7081 aggccgaagg cgatgttttc ctccacagtc aggtgcggga agagcgcgta actctgaaag
     7141 accatcgaaa tgtttcgctt gttggggggg aggttcgtca catccctctc ctcgagaaga
     7201 acccttccgc tggtggggat ctcgaaaccc gcgataattc ttagcgtggt agtctttccg
     7261 cagcccgacg ggccgagcaa cgtcacgaga tgtcccttct ctatcacctg atcgaagtta
     7321 tctacggcca cggtctccac gctgtaactg gtgaagacct ttctaacgtt cttcagaatc
     7381 aaactcatat catcacccct gtcccatgct ttcgctgccg gaaagactga ccttgttctt
     7441 gaggaagatc ttcaaaacca gtatgaaggc cattatcatc gctatcagta tcaccgagaa
     7501 ggcgcaggcc tccgaaagcc ggccggaatc gctctggctg agaatctgaa cggttatcag
     7561 gttccaccgg gccgacacca ggaagatcgc cgcgcttatg gcggtcatcg cccggacgaa
     7621 ggcgaagacg agggctgaga agaaggccgg gactatcaga gggagcgtaa ctctagtgaa
     7681 gacctgacgg ttatccgccc ccagggtgta ggcggcctct tcgatcgccg gatcgacctg
     7741 gtttagcagg gccacccccg actggatacc gacggggacg tatctgaaga cgaagttcaa
     7801 aaccaggatt gccagagtcc cggtgagcag gaagggcttc gtgttgaagg ccaggatgta
     7861 gcctatgcct ataaccgttc cgggaacggc gaaactgagc agggatgtga actccatgag
     7921 cctcctgccg ggaaagatct ttctgaccag cagaaaggcg ataaccattc ccaggagcgc
     7981 agatataggc gtcgatgtga aggcgatgat cagggtatcc ttgatcgctt ccaggccgac
     8041 atcgaagacg tacctgaagt tctccagcgt cagcgtgttg ttcatgcccc aggcctttac
     8101 gaaggctccc cagaagatcg tggcgtagat cgcaagagtg aacgctgcca tcagcatgca
     8161 gagcgagaag agcaaccact tgaccggtct gttcacactc ttgagaaccg aggtggtcgg
     8221 tttgccggtg acggtcacgt acttcttctt tccgacccag tacttctgaa ttatgaaggc
     8281 gactatcgaa gggaataaga gccacacagc gagcgccgcg cccccgcggg tgtcgtacat
     8341 ccccgttatc tggagatagg cctgcaccga tagcaccggg aacttactgc cggccagtat
     8401 cagagggttg ccgaaatcgg ccagcgattc tatgaacaac accaacatcg tgctggcgat
     8461 gccgggaata gacatgggaa gtataacctt gcggaaggcc tgccagcggt tggcgcccag
     8521 gttgaaggcc gcatcctcca gcgtcgggct tatcgtccct atgacaccgt cgagcgtgat
     8581 gaaggcgacc gggaagaagc agaccacctg ggccatcata agtccccaga ggccataaac
     8641 gggaaagttc cggataccga atatcccggc tgttatgaat ccgttgaagc cgaacagcat
     8701 gattatcgac agcgccccta tgaagggcgg aaagactatc gggagaacgg ccaccgttct
     8761 gaagaaccct ttcaggggga tgtccgttct gttgatggtg taggcgaaaa tgtaaccgat
     8821 cagcattccg aaaaaggccg ttagcccggc gacgaggagg ctgttgaaaa agccctgcct
     8881 catgtacctg ttggagaagg ccttcgtgta ggcgcttaga tcgaactgtc cgtttttatc
     8941 tatgagactc gcggagaaga ccttgaaaat cggatagact atgaatatcg ccagcgcggc
     9001 aaagatgatt attatagtta aaagcagaac cgggtccttt agaagattga gcaatctgtt
     9061 cctggtctct tcactcctga aagccacaaa cgctccctcc ccgacatact tcgaaatcgc
     9121 cattaacctg tacgtgaggg cgaacaggag ctcgccctca cggtttggtt catgaaatca
     9181 aattgaccgg gagctctgga gttcccggaa agtctctcaa tcattgaatc agtaaggaag
     9241 aacctcgtca atccatctct cgatgagtct agttttgttt tcggccgccc agaccggatc
     9301 gaccggtagc aacttgatgg tcgagagcgg ctgaaggctg ccgaaatcga cgtccgatct
     9361 tatcgggtag aaataggtct gctggttcac gatctgctgc tgaccggctt tcgatacgac
     9421 ccagtcgatc aatttctgcg cgtcggtcag attcctggca cccttgagta tcgatacgga
     9481 ggccacttcg tatggaactc cctctttggg gaagacgacc tttacggggt aaccctgttc
     9541 catgaactgg aagaaggccg gtgtgaactg gatgcccatc gcgacctgtc ccggtcctac
     9601 ggccttagac ggcccggtgc cgctctgggt gtaggtctgt atgtttggcg ccagcttttt
     9661 gagatagtcg atcgcaccgt cctcaccgta gatttgaata agtcctgtaa tgagactgta
     9721 ggccgttccg gaggattgtg ggttggggta ctggatcatt ctgctcaggc tggtcgagag
     9781 gatatcttcc catgttacgg gcatcgaagc tttgatctgt tccaaaacgg cggtgttgat
     9841 tccgatcccc agaggattca tgtagatcgg gtgatagtag ccttcaacat cgtagaactc
     9901 cggagaaact ccgtacacgc tcagagtctt gtaaggctgg gtgagaccgc gttccttggc
     9961 tattatgtgg ttttccagcg gcgcgccgaa ccagacgtcg gcctgcgggt tgttaacctc
    10021 ggcctcgatc ctggccagtg ccgggccgga agagaggaag acgaagtcga cgcctactcc
    10081 cgtctcctcg acgaaggcgt tgaggatctt cctggcgttc gcctcatcga cactagagta
    10141 cacgaccaaa ttcgcaaggg ttacagcaac gagcattaaa aaaagagata agataagaac
    10201 acgtttcatg atttacacct cctaattcgc ttgaatatat tttagcatcg accacctgaa
    10261 gagaagaaga tccggagagg ctgtcaacat tccccggcca tcacacgaat taagcaaacg
    10321 attctgtgcg ataatcgaag atatagagct ttaatcgcta ttcatcgcct ttgaaactac
    10381 tacatcacct gcattcggag tatgaaactg caatttcata gtgctactat attgaaataa
    10441 tagtttcatt agtttcccgg gtttcgaatt tgataggcca aggaaactcc ttttttgccg
    10501 gaggtaccgg atggtttcaa agaagatctc ggaaatgatg ggtcaatttt cgcctgtaaa
    10561 tagaaagatc gcatcgtgtg tactgagaaa ctacccggag atggggttca tctccataga
    10621 gagtctcagt tcgatggccg gcgtcagtaa agcgtcgatc gtaagatttt ccagagcgat
    10681 aggtttttcc ggtttcaacg aactgaaaaa gcgtattcaa agcgatctca agaagaagct
    10741 gcaaccgtac gagaagatcg ccgcgacgga cctggataaa aagaacgtag acgagcaatt
    10801 gcgggagctg gcgagaaacg agatcgacaa cctgaggaag actctttcga gcatcgaaga
    10861 gggggagctc ctgaaatgga tcggggctat cggcaaggcc aggaatatat atttcgccgg
    10921 attcggtgca accaggcaca tcgtgtctct tatgcagtac actttctcgg tgctccagag
    10981 aaaaccaacc tggttgctgg acggctcgat ctccgatttc tcatacaaga tcaaactcat
    11041 ggataagagc gatatactca ttctcatgac ctttcctcct tactcgaagg agatcgagta
    11101 catagtccgg tttgcaagag agagggatgt agaggttttc ctgatcacgg attcggtgga
    11161 gtgtccggtt tattctcagg cccgttcgac catcgtttgc gagaacaact ccctgttgtt
    11221 cggcaactcc ttcgtgggac cgatagccct gatcgaagtt ataggcaact tcctgatcct
    11281 gggcgagaag gaactgggaa tgactgagat gaaaacgctc tgggaggttg aagaaaaggg
    11341 ttactctttg atagattctc agttcgaaaa gtgagtttga aagtcgtttc attctttacg
    11401 gctgcaccgc ttctggattg gctgcagacg tgttcaggaa tcgaagtggg gaaatgccgg
    11461 tccgggtatc ctgttcgccg gttagagttc taccggcgaa tatcccggcg atacgttgcg
    11521 ggaagtttta cgaaattcta ctatttaact ggaacagagg tgttttcatg aaagctatcg
    11581 tggttcatgg cggggccggt agtttcgaaa gcgaggagga tcacaggaat cataagaggg
    11641 gaatagctga agctctggaa gccggctttt ccatgctcga cagaagaaaa agctctatag
    11701 aaggcgttgt cgccgccgta aagaaaatgg aggaagaccc ggctttcaac tgcggaaaag
    11761 gttctgtact gaacttgaag ggcgaagtgg agatggatgc agcgatcatg gacagcgaac
    11821 tgaacgccgg cgccgtctcc ggcctcaaga gaatcctcca tcccatagag gtggcgagag
    11881 ccgtcatgga gcagacggac aacgttttac tctctggcca ggaagtggag gagttcatcc
    11941 ggatcatggg tttcccgcgc gaggagaacc ttgttgtgcc gacgagacag atgcagtggc
    12001 aaaggaatat ggaaaagatc gagaggggcg agaacctgcg cttctccaga accattgcgc
    12061 tggccagaaa aaccccgggg tattactcca cctccggggc cgttgcgata gacgacaacg
    12121 gaaagatggc cgcggccact tccacgggcg gcatcgtgat gaaaaccttc ggaagggtgg
    12181 gagacactcc cgtaatcggt gcgggtacct acgcaaacaa cttcggagcc gtctcggcaa
    12241 cgggacacgg ggaaaagata ataaagctga cgctttgcag actcgtggcc ttctttatgg
    12301 aacaataccc ggcccagaag gccgtcgatc tggccctgga gagagccaag tatatcgact
    12361 gtgaatgcgg actgatcgcg atcgaccggt atgcaaacat agggataggt tataccacca
    12421 gagatatgtc ctgggcgtac gcaagagaag gccataaacc ggtaaccttt tgacccatac
    12481 ttattcggct cttccgtcgg caatctgtga tcggctctct actctaagct tcttgatcgc
    12541 tcccatcgct aggatcccgg cgacgaacaa cgtgaagaaa tctgccgttg tctgggcgta
    12601 gatgaccccg ttgagcccga aaatacgcgg cagcgttatc acggccggta tgaggaatat
    12661 cccctgcttg gccatagaga ggataccggc cgacaatccc tttcccatgc cctgaaagag
    12721 atagttgaag gtcaaaacga agccgaaggt cgggaaaaag atgctcacgg ctctgagggc
    12781 ctgtgaaccg atctctatga cctcgggatc gtcgctgaat atatttatta tcataccggg
    12841 gaagaccatg cccacgatcc cgaacacgat cgcgaaggcc gtagtaacca taagggaaaa
    12901 cttgagagct tcgaatatcc tcgagtaatt cctggcaccg tagttgtagc cggtaacggg
    12961 ctggaaggcc tggccgtaac cgaagagaac catcattccc agcatcagca ccctcatggt
    13021 tattccgacc gaggcgacgg cgtgatcgcc gtaggcggcc gccgcgttgt tgagcagggc
    13081 gaccgagaag ctagagagaa actgcctcag gaaggtcggc aggccgatct tgaagatctc
    13141 gccgtaaata tgaagcgaaa acctgtaaag ctttatcgac agacgcgtgt aactcttctt
    13201 tcgaagatag tactccagaa gtatcatggt cgatacggac tgggaaatga ccgtggccag
    13261 cgcggcaccg actaccccca tgccaaaacc gaagatgaaa atcggatcga gggcgacgtt
    13321 cagaagagct cccgacacca tggccatcat gaacctcggc gcgtttccct cggctctcac
    13381 caggttgttc atggtcatgt tcatcatgtt cagaatcgat cccgcgatta taaccatcgt
    13441 gtactctctg gcgtactcca ttatcgtttc cgtggcgccg aaggcccgaa gcaaaggatc
    13501 gaggaaggtc agccccagga tggtgaagac gattcccgtc gccagactgg tgaaaaaggc
    13561 cgtcgaagcg gccctgtcgg cctgttccct gtctccggcg cccagcaatc tggaaacgta
    13621 cgaagcgccg ccgacaccgt aagtgaggcc gacggcgccg atcagattga atatgggaaa
    13681 ggcgatggcg atcgctccca tcgcgctcgt gccgatcctt ccgatgaaaa aggcgtctac
    13741 gaggttatag atggcattgg tcaacatggc tatgatagat ggtatggaga ggccgatcaa
    13801 aagggacggg atcttcatct ttcccagcgc cctggccctc tcctccgaac tgctgaactt
    13861 caagacgata cctccattgt attgcgaagc tttgctcttt caataataca ccacgcactg
    13921 caattattta gaatcaggcc tctacattac gctcaaca
//
LOCUS       DAOOLY010000031        13949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4757_length_13949_cov_5.283792, whole genome shotgun sequence.
ACCESSION   DAOOLY010000031 DAOOLY010000000
VERSION     DAOOLY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4757_length_13949_cov_5.283792"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2462)
                     /locus_tag="PLD15_02800"
     CDS             complement(<1..2462)
                     /locus_tag="PLD15_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HPB62857.1"
                     /translation="MQLFNSRLLRERMQRFSFPAGGEFKKKINVIAKWQKSLRDSDLS
                     KTKEISIQGLFLSKFFGEVLGYTTQADGKKEWNLTQHPGNDIDARIPDGSLGFYSSDK
                     STTKAVIELKDAMTSLDRRQSARRENFTPIEQSYLYATKYDGCNWIIVSNIREIRLYN
                     KSKTQSYYESFNVLQLEEDEQFKRFYFLMSKDNLLGDGALSPLDELLQTSEAQEKSIS
                     GDFYSAYRELRLKLFKHLRENNVGYDSSILLEKAQKLLDRVVFILFCEDKGLLPREIL
                     KRAFERGVSSFLDSDQRVWLEMKGLFKAIDRGNDKVSPPINGYDGGLFAYDGLLDSLR
                     IGDAIWKEIVELAGYDFESDLNVNILGHIFEQSISDLEQLRSEFDGLELHTSRANRKK
                     QGIFYTPENITHYIVENTLGRYLEEHPDRLPVIRILDPACGSGAFLNQAHTYLQKAWK
                     SAYEQGEIKSKDARYGTLFDYNPAENDRTILLNNLYGVDLNEESVEITKLSLWLKTAR
                     SDEPLENLDRNVRCGDSLVQSREIAGEKAFEYRKEFGKVFEEGGFDIVIGNPPYVRQE
                     LLSPELKEYCSLNFETYSGVADLYVYFIEKGLALLREGGYLSFIVSNKFLRANYGKKL
                     TGYIQKNYTLVELMDFGDLQVFEDATTYPCIITIKKTIPEGPMTFKYLRLETLEVADL
                     RAELEEKGYEVTITPDSGGWSLENRRSRNLLERIKELGVPLGEYVGEKIYRGIVTGLN
                     EAFVIDGQKRKELIEKDSKSAEIIKPHITGREVRRYSIDWEGKYLIFSRRGIDITRYQ
                     AINEYLEYYRERLEPKKENSQK"
     gene            2632..3267
                     /locus_tag="PLD15_02805"
     CDS             2632..3267
                     /locus_tag="PLD15_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB62858.1"
                     /translation="MDITSSDLELAFLSASYSRVREQFGLIVSEHGLQVAVDSFLVDA
                     LDRIGRSWADGSASLSQVYISGKISERLLDEFARDREHKKDDSARIAIVTLYDRHTLG
                     KRIVRSFLRSSGYEIVDLGSLSSNEELLGAVKKYDVEILLISVLMLHSALKIESFITE
                     LKGRGFQTRVVVGGAPFNADETLWRRVKADRYGKTAAEAIDIINEITGDSR"
     gene            3264..4316
                     /locus_tag="PLD15_02810"
     CDS             3264..4316
                     /locus_tag="PLD15_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB62859.1"
                     /translation="MSEITSMERVLKSLSFQEPDRVPLFLLLTMHGAKELGMTIREYF
                     SSAENVVRGQILLRRKYDNDCYFPFFYAGREIEALGGDVVYSEDGPPNAGEPIIKKVE
                     DIERLAMPDPRTVRPLQSVLKAINALKEDGRGGVPVLGVVISPFSLPVMQMGFERYLD
                     ILYDDRAFFDRLMRFNKEFSVRWANAQIEAGATAICYFDPLSSPSITDVETFKATGYQ
                     IAKEVISRIKAPVAMHFASGLCMDILDLLPATGTVAIGFSALENIPKLKEKASGLKLS
                     LIGNLNGVEMISWDRKKAEETVRRAIEQGAKGGGFVLSDNHGEIPFQVSDEVLKAISE
                     AVHRWGEYPLSEGITR"
     gene            4533..5843
                     /locus_tag="PLD15_02815"
     CDS             4533..5843
                     /locus_tag="PLD15_02815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB62860.1"
                     /translation="MRERPEITHIAYDYCQELVACKVILQQQIERNSYSVTDGWLSQW
                     REHLRNWGFDPSKTLGFMGEFSSSIVHLKTGVIENPERELADFAKAVKLPTDVIDIGL
                     DYAKNLVLASVYDRMARKMGLFEEALDFDLHRQIADYAMINEVLMEIASLNSVNEVLE
                     KISTLAEALFAPEEVLFFEKFRDGWREIYSSARGSTGAEEVLQIPENPEDIFSLEGGR
                     DLWMKVRHDDKLLGALKISKIAFPEYVDRYLAVLGNIRSVLGLSLINVELHEELKTIA
                     LTDPLTGIYNRRGFFDKTKVEFLKASRESKSLSLAIYDLDNFKAINDFCGHEVGDLVL
                     KEVTKIVKDNIRESDVFGRFGGDEFIILFPGCDLKSTLRVLDRIRVAVGKIKEPSLKG
                     RELSLSFGLTTTRDGTGSFEELVALADSALYEAKNSGRNTIKSI"
     gene            complement(5874..6518)
                     /locus_tag="PLD15_02820"
     CDS             complement(5874..6518)
                     /locus_tag="PLD15_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPB62861.1"
                     /translation="MFYLDMTLRLFSALLAGALIGVTRERIHKPAGLRTHALICVGAA
                     FITVLSTTVFITDDHGDPGRVAAQIVSGIGFLGAGTILKKGFSVKGLTTAATLWVTAA
                     VGMGFGAGEYLLSSVVTVFALTIVLFLKKIELLLGGRNLPRIIITARENKDLLGNITN
                     WFKDNAITVESIDVEEDEDNVSILVEISKGDVIKAKQMMIDLTRIEGVRGAEIR"
     gene            6649..7980
                     /locus_tag="PLD15_02825"
     CDS             6649..7980
                     /locus_tag="PLD15_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB62862.1"
                     /translation="MKEFTRRMLSIAIPITLQNLISTGLNLVDNLMVGQLGTTAIAAV
                     GLVNQVVFILNLFTFGASSGAGIFVSQYWGKRDEKSIERTLGHIIYITMGAATVFFVL
                     LYVFPEGILKIFTSDTEVIRLGTEYARPVAFSTFLTSFSFIMAMALRTVEKARIPLYI
                     SLVALSINTVGNYILIFGLGSIPAMGVFGASVATLVARIAEFFIFLYIALRRLTPIRF
                     TLWAFRFDGPFFRRLMKYATPVIFNEFLWSLGVTVYSLVMARMGTEFIAARNISSTIE
                     NFGFVIFGGLASATVVMVGSELGRNNFTQARFNARKLIQLTVITAVATGLMIIFLSRF
                     IVQLYNIDQTLKDIVLTVVVIVGLAQPIKMFNAVNIVGILRSGGDTKAAMLMEIFSLW
                     GVGIPLVAIAGLVLKWSLPAVYIVMMVEELVKAILGIWRTKTGKWVRNVVD"
     gene            complement(8054..9439)
                     /gene="pepV"
                     /locus_tag="PLD15_02830"
     CDS             complement(8054..9439)
                     /gene="pepV"
                     /locus_tag="PLD15_02830"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HPB62863.1"
                     /translation="MDKKLDDLVLSLKDEMVNSISESIKIKSVRENPVEGGPFGEGIK
                     KSLEHTVELAGKLGFQARNAEGYVGIVDFGSGKTFGVLGHLDVVPEGEGWEVPPYSGL
                     VKDGEIWGRGTQDDKGPMIAALYALKAIKEHCQNPSKRIRLIFGTNEESGWECMKYYV
                     KHEEIPDMSVTPDAEFPVIFAEKGIVSYTISSRAGNDGEGLVIESLQAGEAANMVASI
                     ARAVLKGVTPDVFDKIKNFKPSNEARIEVRESAGKVEVSFTGVSAHGSTPYKGHSALA
                     ALVDLLADLPFSDRELGNFLHLVRSKIGYEFFGESLGIAGRDSMSGELTLNLGTMKLG
                     GGEIKLVINIRYPVFFNEAMVRTQIEEAFKGCRVEMHNNKNPLYVSPDSDLVKMCRQV
                     YREMTDHDEDPIAIGGGTYARAVPNAAAFGALFPGGIERAHQPNERVSIDDILMVARI
                     YAQLFLRFLQS"
     gene            complement(9443..10459)
                     /locus_tag="PLD15_02835"
     CDS             complement(9443..10459)
                     /locus_tag="PLD15_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPB62864.1"
                     /translation="MVKTLIMAGGIGERLWPVSSEKRPKQFHHFGSAKTMIEETIERM
                     EKLTSEMLIITTREQFPLIQYYTPLFKGDNVIFEPAGRNTAPAIALGSTRFEDDDIMV
                     IVPSDHIIRDEETFLRTIKTAIEYAGEHEALVTIGITPTSPNTGYGYIERGACLSEHE
                     EVFAVRKFHEKPDYETAQQYFQSGKFYWNSGMFVWKKRVFDRELSKHLPELYEAMLEL
                     KNNPSRIEEIYEKVPRISIDYGLMEKTSNVATVPASFYWNDIGSWDAVYELLHKDGNG
                     NAVIGKFSTHDVKNCLLVNYTQTRVGISSIEGYIVVMSGEGTLVCRRGESQTIKEIIK
                     QQGG"
     gene            10677..11069
                     /locus_tag="PLD15_02840"
     CDS             10677..11069
                     /locus_tag="PLD15_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPB62865.1"
                     /translation="MNKVILRIRMSLHDAHYGGNLVDGAKLLQLFGDVATELLIRNDG
                     DEGLFRAYDSIEFLSPVYAGDYIEVTGEIVSRGRTSRKMVFTATKVIQARPDISDSAA
                     EVLDEPVVVCRASGTCVVPLDKQRQKDE"
     gene            11062..11877
                     /locus_tag="PLD15_02845"
     CDS             11062..11877
                     /locus_tag="PLD15_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPB62866.1"
                     /translation="MNKLIITAALTGAEVTREQQPALPITPGEIAQAAYDCYNAGASV
                     IHVHARDMDGKPTQRKEAYAEIRERIAEKCCIIFQPSTGGAVWHTAQERGGPLELDPE
                     MATLSTGTCNFGDDVFFNSQEIIEHFAREMLSRKIKPEIEIFERGMIENALRLVKKGL
                     LVPPVHFDFVLGVPGACPGNIEDLLHMVRSIPQGSTWTVAGIGRYELPLAVHAIPMGG
                     HVRVGFEDNIYYRKGELATSNAQLVERIARIAREYGREIASPDEARAILGISN"
     gene            11892..12923
                     /locus_tag="PLD15_02850"
     CDS             11892..12923
                     /locus_tag="PLD15_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB62867.1"
                     /translation="MKGCPFGSHRVIEPEGSLPQAAKRIDNTPRIRDNEILIDVQTLN
                     VDSASFTQIKSVCGDSEEAVAAMITDIVRSRGKLQNPVTGSGGMLIGRVAEIGSLLKG
                     RDLEAGDRIATLVSLSLTPLLIEKIGKIHMDKDQVDIRGKAVLFESGIYARLPEDMPD
                     RLALAILDVAGAPAQVDKLVKPGMNVAVIGAGGKSGLLCCYQAKKKAGAGGRVIAVEY
                     SPENADRLSRMGLADHTIVADATRPLEVYEKVKEITGEALCNVVINNVNVQATEMSSI
                     LVTREGGIVYFFSMATSFARAALGAEGVGKDITMIVGNGYTRGHASLALDILRESVAI
                     RELFERLYV"
     gene            12934..>13949
                     /gene="ablA"
                     /locus_tag="PLD15_02855"
     CDS             12934..>13949
                     /gene="ablA"
                     /locus_tag="PLD15_02855"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPB62868.1"
                     /translation="MRDYRDIPLWKDVTSEQWSDWRWQVANRVKSVEALRQVIEITEE
                     EALGISECLRTLRMSITPYYATLMDPRNERCPIRRQAVPTDRELNIDRWDMVDPLHED
                     EDSPVSGLTHRYPDRVLFLITDQCSMYCRHCTRRRFAGQLDRPRTKKEIDAAVEYIKE
                     TPEVRDVLLSGGDALLVGDDFLEYILSELRRIPHVEIIRLGTRAPVVLPQRITPELVA
                     MLRKYHPVWINTHFNHPLEITPDSKKACEMLSDGGIPLGNQSVLLRGVNDSPYIMMEL
                     VHRLVEIRVRPYYIYQCDLSQGIGHFRTSIRKGIGIMEALIGNTSGFCVPTFVVDAPG
                     GGGK"
BASE COUNT         3204 a         3508 c         3761 g         3476 t
ORIGIN      
        1 atcttctggg aattttcttt cttcggctca agcctctctc ggtagtattc gagatattca
       61 tttatcgcct ggtatcgagt tatatctatt cctctccttg aaaatatcaa atacttccct
      121 tcccagtcga ttgagtagcg tctgacctct ctgccagtta tgtgaggttt gattatctcc
      181 gccgatttac tgtctttctc gatcagttcc ttccgctttt gtccgtctat aacgaaagcc
      241 tcgttcagac cagtgacaat tccacgataa atcttctcac ccacatactc gcccagagga
      301 actccgagct cttttatcct ctccagcagg tttctggatc tccggttttc gagtgaccaa
      361 ccgccggaat cgggcgttat agtgacttcg tagcccttct cttcgagttc cgctcttaga
      421 tcggctactt cgagggtttc caatctcagg tacttgaaag tcatcggtcc ttcgggaatg
      481 gttttcttta tagttattat gcagggatag gtcgttgcat cttcgaagac ctgcaggtct
      541 ccgaaatcca tcagttccac aagcgtgtag ttcttctgta tatatcctgt cagctttttt
      601 ccataattgg ccctgagaaa tttgttggat actatgaagc tcagataacc gccctcgcga
      661 agaagcgcca gacccttctc aatgaaatag acgtagagat cggccacgcc gctgtaggtt
      721 tcgaaattga gggagcagta ttccttcagt tccggtgaaa ggagttcctg cctgacgtaa
      781 ggggggttgc ctatcactat atcgaaccca ccttcttcga agacttttcc gaactctttg
      841 cgatactcga aagccttctc gccggctatt tctctcgatt ggacgagcga gtcaccacaa
      901 cgaacgtttc tgtcaagatt ttccagcggc tcatcacttc tggcagtctt caaccagagt
      961 gaaagtttgg tgatctcgac gctctcctcg ttcagatcta caccgtacag gttgttgagg
     1021 agaatcgttc tgtcgttttc cgccgggttg taatcgaaga gagttccata cctggcatcc
     1081 ttgcttttga tttccccctg ttcgtaggcg ctcttccagg ccttctgaag ataggtatgc
     1141 gcctggttga ggaaggcgcc gctgccacag gcggggtcga gtatccgtat gacgggtaat
     1201 ctatcggggt gttcttccag atatcttccc agagtgtttt ccacgatgta gtgagttatg
     1261 ttttcaggag tgtagaagat cccctgtttc ttgcggttcg ccctgctcgt atggagttcc
     1321 agcccgtcga attcgcttcg taactgttca agatcggaga tcgattgctc gaagatgtgt
     1381 cccagtatgt tgacgttaag atcgctttcg aagtcgtacc cggccagttc cactatttct
     1441 ttccagatcg cgtcgcctat acgcagcgaa tcgagcaggc cgtcataagc gaagagtccc
     1501 ccgtcgtagc cgttgatcgg cggcgacact ttgtcgtttc ctctgtctat ggccttgaaa
     1561 agacccttca tttcgagcca gactctctgg tcggagtcca ggaaggagga gacccctcgc
     1621 tcgaaggccc ttttgagtat ctccctgggt agaagtcctt tgtcctcgca gaagagaatg
     1681 aagacgaccc gatcgagtaa tttctgggcc ttttcaagaa gaatagaact gtcgtatccg
     1741 acgttgttct ccctgagatg cttgaatagc ttcagacgga gctccctgta agcgctgtag
     1801 aaatccccgc ttatgctctt ttcctgcgcc tcgcttgtct gcaaaagctc gtccaaaggt
     1861 gatagcgcac cgtctccgag cagattatcc ttcgacatga ggaaatagaa gcgcttgaat
     1921 tgctcgtctt cttcgagctg gagaacgttg aaactttcgt agtagctctg ggttttcgat
     1981 ttgttgtaga gccttatttc gcgtatgttg gatactatta tccagttgca gccgtcgtac
     2041 ttggtagcgt aaaggtaaga ctgttcgatg ggagtgaagt tctctctccg ggcagactgt
     2101 ctcctgtcca gagaagtcat ggcgtctttc agctctatta cggctttcgt tgtactctta
     2161 tcgctcgagt agaaacccag agaaccatcg ggaatcctgg cgtctatgtc gttgcccggg
     2221 tgttgcgtca ggttccattc cttctttcca tcggcctgcg ttgtgtagcc gagtacctcg
     2281 ccgaagaatt tagagagaaa caggccctgt atcgaaatct cttttgtttt actcaagtcg
     2341 ctatctctca aagacttttg ccacttggct atcacattta tctttttctt aaactccccg
     2401 ccggcgggaa aggagaatct ttgcatcctc tctctgagaa gccttgagtt gaaaagttgc
     2461 attcagtgcg ccccccgatt gaaacgttct cgaaaaagta tagcacggat cgctctacca
     2521 aaaaaaacgg tcaactgtcc ggaattgtag agtcggtggc actgttttgc atgttttttg
     2581 tgtaaaatgg agcataaacg agagagttct cttttcacag gagtggttga ggtggatata
     2641 acttcgagcg atctggaatt agcgtttctt tcagccagtt attccagagt cagagagcag
     2701 ttcggcctga tcgtgtcaga acacggttta caggtggctg ttgacagttt cctggtagat
     2761 gcgctggata ggatcggccg gtcttgggca gacgggagcg cctcgctttc gcaggtctat
     2821 atctcgggca agatctccga acgtcttttg gatgagttcg cccgcgatag ggagcataag
     2881 aaagacgaca gcgcacgaat cgcgattgtg actctgtacg atcgacacac tctcgggaag
     2941 aggatagtga ggagtttcct gagatcttca ggctatgaaa tagtggatct gggatcgctt
     3001 tcaagcaatg aagagctact tggcgccgtt aaaaaatacg acgtggagat tcttctaatc
     3061 tctgttttga tgctccactc tgccctgaag atcgagagct tcatcacaga gctcaaagga
     3121 aggggatttc aaacaagggt ggtcgtcgga ggtgcgcctt tcaacgccga cgaaacactc
     3181 tggagaagag ttaaagccga tagatacgga aagacggcag ccgaagctat cgatataata
     3241 aacgagatca cgggagattc gagatgagcg aaataacttc gatggagaga gtcctaaaat
     3301 ccctcagctt tcaggaaccc gacagggtac cgctattctt gcttctgact atgcacggcg
     3361 cgaaggaact tggcatgacc atacgtgagt atttttccag cgcggagaat gttgtcaggg
     3421 gtcagatctt actgcgaaga aagtacgaca acgattgtta ttttcccttc ttctacgccg
     3481 ggagagagat agaggcgctt ggcggcgatg tagtttacag cgaggatgga cctccaaatg
     3541 caggggagcc gatcataaag aaagtcgaag atattgaaag attggcgatg cctgatccga
     3601 gaacggtccg ccctctacag agtgtgttga aggcgataaa cgctctcaag gaggatggaa
     3661 ggggtggtgt tccggtctta ggtgttgtca tttcgccctt ttcgctgccg gtgatgcaga
     3721 tgggcttcga gagatacctg gatatccttt acgatgatag ggcctttttc gacagactga
     3781 tgagattcaa caaggagttc agtgtgaggt gggcaaacgc tcagatagag gccggagcga
     3841 ccgcgatctg ttatttcgat cctctttcct cgcccagcat aacggacgtg gaaactttca
     3901 aagctactgg ataccagatc gcgaaggaag ttatctccag aataaaggca cccgtggcga
     3961 tgcatttcgc ttcaggtctt tgtatggata ttctggattt gctcccggca accggaaccg
     4021 ttgcgattgg cttcagcgct ctggagaaca taccgaaact gaaggagaaa gcttcgggtc
     4081 tgaaactctc gcttataggc aatttgaacg gtgttgagat gatatcctgg gatcgaaaaa
     4141 aagccgaaga gactgtcaga agagcgatcg agcagggggc aaagggtgga gggtttgttc
     4201 tgtcggataa ccacggcgaa atccctttcc aggtttccga tgaggttttg aaggcgattt
     4261 ccgaggccgt gcatagatgg ggcgagtacc cgctctcgga aggaattacg cgatgaagaa
     4321 gatcgtggtg gtagcctgct ccaatttcca acaggagatg cttaagataa gcacccacaa
     4381 tgagtttcag gggatgagtt tttgctttta tccggctttg tgcgaagttt cccccgtcag
     4441 atacgcgcga aattctccac gatgttttga gctcttcggg cgagaagttt cacttccttt
     4501 tcgctcccag gtcctgtccg gttttaaaat acatgcgtga aagacctgaa ataacgcaca
     4561 ttgcgtacga ttattgccag gagctcgtgg cctgcaaagt cattttgcag cagcagatag
     4621 aaaggaattc ctattcagta acggatggct ggctttctca atggagagag catttgagaa
     4681 actggggctt cgatccttct aagaccttgg gcttcatggg agagttctcc tcatcgatcg
     4741 ttcaccttaa aaccggggta atcgaaaacc cggaacgtga actcgccgat tttgccaaag
     4801 ccgtaaagct cccgacggat gtgatcgata tagggcttga ttatgcgaaa aacctcgttc
     4861 tggcctctgt ttacgacaga atggcgcgga agatgggcct tttcgaagag gcgctcgatt
     4921 tcgatctcca caggcagata gccgattatg cgatgatcaa cgaagtcctc atggagatag
     4981 ccagtctgaa ttctgtgaac gaggtcctgg agaaaatatc cacactcgcc gaagcgcttt
     5041 tcgctccaga ggaagtcctt ttcttcgaaa agtttcggga cggctggaga gagatttact
     5101 catctgcaag aggctcgaca ggcgccgaag aggttctaca aatcccggaa aatcccgaag
     5161 atatcttctc gctcgaaggc ggcagagacc tctggatgaa ggtccggcat gacgataaat
     5221 tgctcggagc gttgaagatc tcgaaaatcg cctttccaga gtatgttgat cgctatctcg
     5281 ccgtactggg aaatatcagg agcgttctgg gtttatcgct catcaacgtt gaactccacg
     5341 aagagttgaa aacgatcgcc cttacagatc ctctgacagg aatttacaac aggagaggct
     5401 tcttcgacaa gacaaaagtc gagtttctta aagcatccag agaaagcaaa agcctttctc
     5461 tggcgatcta cgatctcgac aacttcaagg cgataaacga tttctgcggc cacgaagtgg
     5521 gtgatttagt tctcaaagag gtcactaaaa tcgtcaagga taatataaga gagtccgatg
     5581 tgtttggcag gttcggagga gatgaattca taatactctt ccccggttgc gatttaaaat
     5641 ccacgctgag agtgctagat aggataagag tggctgtagg aaagattaaa gaaccttcgc
     5701 tgaagggacg ggagttatcg ctcagcttcg gactcactac cacaagggac ggcacaggct
     5761 ccttcgagga actagtcgct cttgccgaca gcgctctgta cgaggcgaag aactctggaa
     5821 gaaacaccat aaaatctata tgatcttatt gtgttcgatc tgcaagtttc gctctagcgt
     5881 atttccgccc ccctgactcc ttctattctt gtgagatcga tcatcatttg tttggcctta
     5941 atcacgtcgc ccttgcttat ctcgacgagt atgcttacgt tgtcctcgtc ctcttcgacg
     6001 tcgatcgact cgacggtgat ggcgttgtct ttgaaccagt tagtgatgtt gccgagcagg
     6061 tctttgttct ctctggcggt tattattatc ctcgggaggt ttctgcctcc caggaggagt
     6121 tcgattttct tcaggaagag gacaatcgtg agggcgaaaa ccgtcacgac cgaagagagc
     6181 agatactctc ccgcgccgaa acccattccg acggcggccg ttacccagag tgtggcggct
     6241 gtggtcagtc ccttcaccga gaagcccttt ttcaatatcg ttccggcgcc cagaaaacct
     6301 attcccgaaa cgatctgcgc tgcgactctc ccggggtcgc catggtcgtc cgttataaat
     6361 accgtggtcg atagtaccgt tatgaaggcc gccccgacac agatgagggc gtgtgtcctc
     6421 aatccggccg gtttatgaat cctctctctg gtgactccga tcagcgctcc ggccaacagc
     6481 gcgctgaaga gcctcagtgt catgtccaga tagaacaagt tcctccctcc ctttgaagct
     6541 agattataac ccattttacc cggcgcgtgg caattcgata cactctcaag ttgtcttctg
     6601 aaccgataga ctgtataatt ctacggcgtc tggttttagg aggcactcat gaaagaattc
     6661 acacggcgga tgctttctat tgcgatcccc ataacactac aaaatcttat atccacaggt
     6721 ctcaacctgg ttgataacct gatggtcgga cagcttggca caacggcgat cgcggccgtc
     6781 ggactggtta accaggtagt ctttattttg aacctcttca ctttcggggc atccagcggc
     6841 gccgggatct tcgtttccca gtactggggc aagcgcgacg aaaagagtat agagagaacg
     6901 ctgggacata taatatacat caccatgggt gcggcaacgg tcttcttcgt gctcctatac
     6961 gtctttcccg aggggatttt gaagatcttc acctccgaca ccgaagtgat aaggctcggt
     7021 acggaatacg cacgtcccgt ggccttctcg acttttctga cgtctttctc cttcattatg
     7081 gcgatggctc tgagaacggt cgaaaaggcg cggataccgc tgtacataag tctggtggcc
     7141 ctttcaataa acaccgtggg caactacata ctgatcttcg gtctcggttc gattccggcc
     7201 atgggagttt tcggcgcttc cgtcgccaca ctggtggcca gaattgccga gtttttcatc
     7261 tttttgtata tcgccctgag aagattaaca cccataaggt tcaccctgtg ggccttcagg
     7321 ttcgacggcc catttttcag gcggctaatg aagtacgcga cgcccgtgat cttcaacgag
     7381 tttctctgga gcctgggagt cacagtctat tcgctggtga tggccaggat ggggactgag
     7441 ttcatcgccg ccaggaacat ttccagcacg atagaaaact tcgggttcgt tatctttgga
     7501 ggactggcat cggccacggt ggtgatggtg ggctcagagc tgggaagaaa caatttcacg
     7561 caggccaggt tcaatgcgcg aaagctgatt cagcttaccg tgatcacggc tgtcgccacg
     7621 gggctgatga taatcttcct ctcgcgtttc atcgtacagc tctataacat cgatcagact
     7681 ctcaaggata tcgtgctgac agtagtagtc atagtgggac ttgcccaacc gataaagatg
     7741 ttcaacgccg tcaacatcgt ggggatattg cggagcggcg gcgatacgaa ggccgccatg
     7801 ctaatggaga tcttctctct ctggggtgtc ggaatccccc tagtcgcgat cgccgggctg
     7861 gttctgaagt ggtctcttcc ggccgtttat atcgtgatga tggtcgagga gctggtcaag
     7921 gcgatactgg gcatctggag aacgaagacc gggaagtggg tacggaacgt ggtcgattga
     7981 agccgcaggt tttcaaataa aacgggcgca ccggccggtg cgcccgttgt taggcttgat
     8041 ttactctctc acttcaagat tgcaggaacc tgaggaagag ctgagcgtaa atcctggcga
     8101 ccatcagtat atcgtctatg gaaactctct cgttcggctg gtgagccctc tcgatgcccc
     8161 cggggaacag agccccgaag gccgccgcgt tcggcaccgc ccttgcgtaa gttccgccgc
     8221 cgatggctat cgggtcttcg tcgtggtcgg tcatctctct gtacacttgc ctgcacattt
     8281 tgaccagatc ggagtcgggc gagacgtata gagggttctt gttgttgtgc atctcaaccc
     8341 tgcacccttt gaaggcttct tcgatctggg tccttaccat ggcttcgttg aagaagactg
     8401 gatagcgtat gttgatgacc agctttatct cgccgccgcc gagcttcatg gttccgaggt
     8461 tgagtgtcaa ttcgcccgac atcgagtccc ttccggctat ccccagagat tcgccgaaga
     8521 attcgtagcc gatcttcgac ctcaccagat gcaggaaatt gcccagctct cgatcggaga
     8581 agggtagatc ggccagtaga tctaccagag cggctagagc gctgtgaccc ttgtaaggtg
     8641 tcgaaccgtg ggccgatacg cccgtgaaag agacttccac ctttccggcc gattccctga
     8701 cctcgattct tgcctcgttc gagggcttga aattctttat tttgtcgaaa acgtccggtg
     8761 ttacgccctt caaaacggcc ctggcgatcg atgccaccat gttggccgcc tctccggcct
     8821 ggagcgactc tatcaccagt ccctcgccgt cgttgcccgc gcgggaggat atcgtataac
     8881 tgacgattcc cttttcggcg aagatgacgg gaaattcggc gtcgggagtg accgacatgt
     8941 cgggtatctc ttcgtgcttg acgtagtact tcatgcactc ccagcccgac tcttcgttgg
     9001 ttccgaagat aagcctgatt ctctttgagg ggttttgaca gtgctctttg atggctttga
     9061 gagcgtagag ggccgctatc attgggccct tgtcgtcctg agtgcctctc ccccatatct
     9121 ctccatcctt aaccaggccg ctgtatggag gcacttccca gccttcgcct tccggcacga
     9181 catccagatg gcccagaact ccgaaggtct ttcccgagcc gaaatcgact atcccgacgt
     9241 aaccttcggc gtttctggcc tgaaagccca gttttccggc cagttccacg gtatgttcga
     9301 gcgatttctt aatgccctcg ccgaaaggtc ctccctccac cggattctcc ctaaccgatt
     9361 tgattttgat ggattcggaa atcgaattca ccatttcgtc tttcaatgaa agtaccagat
     9421 cgtccagctt cttgtccatc gttcatcccc cttgctgttt tatgatctct tttatagtct
     9481 gcgactcgcc ccttctgcat accagcgtac cttcgcccga cataacgacg atgtagccct
     9541 cgatgctcga gataccgact ctggtctgcg tatagttgac cagaaggcag ttcttcacgt
     9601 cgtgcgtcga gaacttcccg atgacggcgt ttccatttcc atctttgtgg agaagttcgt
     9661 acaccgcgtc ccaggagcct atatcgttcc agtagaagct cgcggggacc gtggcgacgt
     9721 tcgaggtttt ctccatcagg ccgtaatcta tcgatatgcg cgggaccttt tcgtagatct
     9781 cttcgattct cgacggattg tttttcaact ccagcatcgc ttcgtacagc tccggcaggt
     9841 gtttcgaaag ctccctgtcg aagactcttt tcttccagac gaacataccg gaattccagt
     9901 aaaacttacc cgactggaag tattgctggg ccgtctcgta atccggtttt tcatggaact
     9961 tcctgacggc gaaaacctct tcgtgctcgc tcaggcaggc tcccctctct atgtaaccgt
    10021 agcccgtgtt gggtgaggtg ggagtgattc cgatagttac cagcgcttcg tgttcaccgg
    10081 cgtattctat ggccgtctta atggtccgca ggaaggtttc ttcatccctg attatgtggt
    10141 ccgacgggac tatgaccatt atgtcgtcgt cctcgaacct ggtgctcccg agggcgatcg
    10201 cgggtgcagt gtttcttccg gccggttcga atatgacgtt atctcccttg aatagagggg
    10261 tgtagtactg gatcaagggg aactgctctc tggtagtgat aataagcatt tcgctcgtca
    10321 gtttttccat tctttcgatg gtctcttcaa tcatggtttt ggccgatccg aaatggtgga
    10381 actgcttagg tctcttctcg ctgctcacgg gccagagtcg ctcgccgata cctcccgcca
    10441 tgatgagggt cttaaccata aattctcccc ctttttctcg attcgctgtt aatagcatac
    10501 cagaaaatgg ctgctttcca cggaggttac gggagttgta gcggtaaatg acgcatgacc
    10561 gtaaagaaag aaggacatag agtacaatat tgagttccac atgcctctaa ccggtgttgg
    10621 tcaactttgc cagggcaggc tcgaaaaggc aaaattgtat aaagggggta tgcaaagtga
    10681 acaaagtgat tctcaggatc agaatgagcc ttcacgacgc tcactacgga ggcaatctag
    10741 tcgatggggc gaagctcctg cagctgttcg gcgatgttgc gaccgagctg cttataagaa
    10801 acgatggcga cgagggactg ttcagggcct acgacagtat cgaattcctc tctcccgtgt
    10861 acgccggcga ttatatagag gtcacgggcg agatagtttc caggggcagg acatctcgaa
    10921 agatggtttt cacggctaca aaggttatac aggccagacc tgatataagc gattcggcgg
    10981 ccgaggtgct ggacgagccg gtagtcgttt gcagggcgag tggtacatgt gtggttccgc
    11041 tggataagca gaggcaaaaa gatgaataaa ctcataataa cggcggccct gactggggcc
    11101 gaggtgacca gagagcaaca accggccctg ccgataacgc ccggggagat cgcgcaggcg
    11161 gcctacgact gttacaacgc cggcgcgagc gtaattcacg ttcacgcgcg cgacatggac
    11221 ggcaagccca cccagagaaa agaggcgtac gccgagatcc gggagaggat agccgaaaag
    11281 tgctgcatca ttttccagcc ctccaccggc ggcgcggtat ggcacacggc tcaggagagg
    11341 ggaggccctc tagagctcga tcccgagatg gcgacgctct cgaccgggac ctgcaatttc
    11401 ggagacgacg tttttttcaa ctctcaggag atcatagagc atttcgcgcg ggagatgctc
    11461 tcgcgtaaaa taaagcctga gatagagatt ttcgaacgcg gaatgataga aaacgccctg
    11521 agactggtga agaaagggct gctcgtaccg cccgttcatt tcgatttcgt tctcggcgta
    11581 cccggcgcat gccccggtaa catagaagat ctgttgcata tggtaagatc tattcctcaa
    11641 ggcagcacct ggacggtggc cggtatcggg cgttacgagc ttccgctggc ggtccacgcc
    11701 ataccgatgg gcggccacgt aagggtgggg ttcgaagata atatatacta ccggaaaggc
    11761 gagctggcga cctccaacgc ccagctggta gaacgtatcg ccagaatcgc gagggagtac
    11821 ggaagagaga tcgcctctcc tgacgaggcc agagccatac tgggaataag taactgaggc
    11881 gggaggtaag catgaaaggg tgtccgttcg gaagccacag agtcatcgag ccggagggga
    11941 gtcttcccca ggcggcgaaa cgcattgata acacgccccg cataagggat aatgagatac
    12001 tgatagatgt gcagacacta aacgtcgatt ctgccagttt cacgcagata aagagtgttt
    12061 gtggcgacag tgaagaagcc gtcgccgcta tgataacaga tatcgtcagg agcaggggaa
    12121 agctccagaa tccggtcacg gggtcgggcg gcatgttgat cggaagagtc gcggagatag
    12181 ggagtctcct gaagggcagg gacctcgaag cgggcgacag gatagcgacc ctggtttcgc
    12241 tgtctttaac gcctctcctg atagagaaga tagggaagat ccacatggat aaggatcagg
    12301 tagatattcg cggcaaagcc gtgctttttg agagcggcat ctacgccagg ctgcccgaag
    12361 atatgcccga cagactggcc cttgccattc tggacgttgc cggtgcgcct gcccaggtag
    12421 ataaactggt gaagccggga atgaacgtgg cggtgatcgg tgccggtgga aaatccggtc
    12481 tgctctgctg ttaccaggcc aaaaagaaag cgggggcggg aggaagggtt atagcggtcg
    12541 agtattcgcc cgaaaacgcc gacagacttt cgagaatggg tctggccgat catactatag
    12601 tcgccgacgc caccagaccg ctcgaggtgt acgagaaagt gaaggagatc actggagagg
    12661 ccctctgcaa tgtcgtgata aacaacgtca acgttcaggc gaccgagatg agctcgattc
    12721 tagtcacccg cgaggggggg atcgtttact tcttcagcat ggcgacctct ttcgccagag
    12781 ccgcgctcgg agccgaaggc gtcggtaaag atataaccat gatagtcggt aacggttaca
    12841 ccaggggcca cgcttccctc gcgctggaca ttttgagaga gtccgtggcc atcagggagt
    12901 tgttcgagag attatacgtt tgaggtgaga catatgagag attacagaga tatcccgctc
    12961 tggaaagacg tcacctccga gcagtggagc gactggcgct ggcaggtggc aaacagggtg
    13021 aaaagcgtcg aggcgctccg gcaggtaata gagataaccg aggaggaggc tctgggcatt
    13081 tccgagtgtc tgagaacgct cagaatgtcc atcactcctt actacgcaac tctcatggac
    13141 ccgagaaacg agaggtgtcc tataaggagg caggccgttc cgacagacag ggagttgaac
    13201 atagacaggt gggacatggt cgatccgctc cacgaagacg aggactcgcc cgtttccggt
    13261 ctgactcacc gctatcccga cagggtgctt tttctcatca ccgatcagtg ctcgatgtac
    13321 tgcaggcact gcacgaggag aagattcgcc ggtcagctag acaggcccag gaccaagaag
    13381 gagatagacg cggccgtgga gtacataaag gaaactcccg aggtgaggga cgttctcctc
    13441 tcgggcggcg acgcgttgct tgtgggagac gacttcctgg agtacatact gagcgagctt
    13501 cgcagaatac cccacgtgga gataataagg ctgggcacca gagccccggt ggtcctgccc
    13561 cagaggataa cgcccgaact ggtcgccatg ctgagaaagt atcatcccgt gtggatcaac
    13621 acccatttca accatccgct ggagataacg cccgattcga agaaggcctg cgaaatgctc
    13681 tcggacggag gcataccgct gggcaatcaa tcggttctcc tcaggggagt aaacgattcg
    13741 ccttacataa tgatggaact ggtccacagg ctcgtggaga taagggtcag gccgtactac
    13801 atataccagt gcgatctctc gcaggggatc ggacatttca gaacctcgat caggaagggc
    13861 attggcatta tggaggccct gatcggaaac acatccggct tctgcgttcc gaccttcgtc
    13921 gtcgatgcgc cgggcggcgg cggcaagat
//
LOCUS       DAOOLY010000032        13708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4891_length_13708_cov_5.120560, whole genome shotgun sequence.
ACCESSION   DAOOLY010000032 DAOOLY010000000
VERSION     DAOOLY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_13708_cov_5.120560"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1195)
                     /gene="prfB"
                     /locus_tag="PLD15_02860"
     CDS             complement(83..1195)
                     /gene="prfB"
                     /locus_tag="PLD15_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPB62869.1"
                     /translation="MISYEINSKIQELRDKFDSIKGTLDLVRMNEKLKGIEHKMSDPS
                     IWNDKREASNLGKEAQSFRSTLELLKDVEGEFENIDIAVELASEDESYVRHVEELVAL
                     ASRKVREFELTILLSGHYDNNNCFVSIHPGAGGTESQDWASMLMRMYTRWAEANRYKV
                     TIVDELPGDEAGIKSVTLNFAGPYAYGKLKFEAGVHRLVRISPFDANHRRHTSFASVS
                     VFPEMEEIPEIDIRPEELKIDTYRSGGAGGQHVNKTDSAVRITHLPTGIVVACQTERS
                     QHQNKANAMKMLYAKLFEIELEKKRNEKLKLMGDQKDISWGNQIRSYVFQPYTMVKDH
                     RTEIETGDIQSVMDGDIEEFIEKELLFFSAIEKSLE"
     gene            complement(1210..3660)
                     /gene="secA"
                     /locus_tag="PLD15_02865"
     CDS             complement(1210..3660)
                     /gene="secA"
                     /locus_tag="PLD15_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPB62870.1"
                     /translation="MSIFDKIFDKNKMLLKKYSKVVDKINSLEPSVSKLPQEEFLKRT
                     EELKKAAESGPLEDLIPEAFALVREAAKRTTGMRAFDVQLMGAMALNEGKIAEMKTGE
                     GKTLVATMPLYLNALTGKGCHLATVNDYLAKRDAGWMGPVYEYLGMKAGFIQANMDPV
                     TRKEAYNCDITYGTANEFGFDYLRDNLVYSLENKAQRGHNFVIVDEADSILIDEARTP
                     LIISGPAEDSSSLYRQFAFFAKRFQQEKDFLVDEKDRTVTLTDEGIAKAEKLLQIDNL
                     YDPSNYDYLFHLLNALKAMTLYRKEVDYLVSQEGEVVIVDEFTGRLLQGRRYSEGLHQ
                     AIEAKENVQIRQESITFATITFQNYFKLYEKVSGMTGTAATEESEFISMYNTPVAVVP
                     TNREVVRGDKEDLIYKSREEKDEAIIEEIVRRYEKGQPVLVGTTSIEKSEQLSNLLQK
                     KGVAHEVLNAKYHEREAEIVAKAGQAKTVTIATNMAGRGTDIKLGEGVVDLGGLFVLG
                     TERHESRRIDNQLVGRSGRQGDPGESRFYLSTEDDLLRLFGGERMQSIMTTLKIERGQ
                     PIEHPLLSRIISSSQKKIEGIHFEIRKRLFELDSVMDQQRSAIYAHRDWVLKGEELDS
                     NISEIIRDVVERRLDGLQEMPSREEIRNSFAFLPPDSLARLSESKTVQELKESAIKSL
                     EDTYQEKKKSFGEEFPQVMKYIMLRMIDERWRRHLEAVDHLKDSVGLRAYGQKDPVVE
                     FKKESYELFQQLTDSLYDDIASAIVRIVKVDSDKAQQKADKEFKNLQAVHSEFGTADK
                     KSDSTGKKKGTKRFKVKR"
     gene            complement(3670..5091)
                     /locus_tag="PLD15_02870"
     CDS             complement(3670..5091)
                     /locus_tag="PLD15_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62871.1"
                     /translation="MIHFSGVAAQELFDFVPESAGYFVVLRSMRSTLETLRDSTNFFG
                     AYLGDEGFGAERALYGIIDATGYNAEVDTSLLKDALNRDLLLAGEGLELSLEDLLTFD
                     PFYLLEKLKLSRARIFIVWKSESSFTLLKAVAALLDMTIFAQPGESINELRSNSGTLF
                     YYTGEGFLIIGGNREAVQTALNTYQGQERRLLESSEKGREIAEMSYDYTITGFFDSDR
                     FKIDLGLQSPFSVDNTVVFARPEGHTLSATIVQDTIFLNSQDLEVFLKLADTKESEAS
                     EKTHGDYTVFFPSSSVETLRNELTHWFELDLEPYRELADYIVLLSRESTGNARIYGEL
                     VSEVPKVSAVFKNRNGDTSFLESPLKEWGAKTSIYRGNRVYELENDFDTIYFIFSPAT
                     TVLTTLTPEEYYSTVSSSGTLRDNVSYEFVRELGMEKDIVEAFVDVKKIFKSLIGITL
                     DSAIFYQQTFLENGKMVHALRFY"
     gene            complement(5344..5982)
                     /locus_tag="PLD15_02875"
     CDS             complement(5344..5982)
                     /locus_tag="PLD15_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPB62872.1"
                     /translation="MSSKLRLDELLFGKGMSESRSKASELIKAGRVSVEGSIVEKPGF
                     RVDVESRIAITGGKQPVGRGYYKLEKALEFFGLDVSGKEVVDLGSSTGGFTQLLLEKG
                     ARRVVAVDVGKNQLHPSLKNDERVVSLEERDLRELTLEMVGGSVDLVTVDLSFIPTSS
                     VAREISELLKDGGLCVSLVKPQFEAGPGKIHKGVVKSHAIHVEVLERSIKAF"
     gene            complement(5966..6718)
                     /gene="truA"
                     /locus_tag="PLD15_02880"
     CDS             complement(5966..6718)
                     /gene="truA"
                     /locus_tag="PLD15_02880"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPB62873.1"
                     /translation="MKRFAAVVSYDGTDFFGFQNQPEMRTVQGTFEEALERIHKYRIV
                     TQGAGRTDTGVHGYGQVIAFDSSLDHLSAKIMCDALNANLPADIYVRRVHEVESNFSP
                     RFAAKKRIYHYHILNSSEPDIFRRRYVWWFPYGLDIEAMRKAAGHLTGEHDFSAFKTG
                     RDDRNPNRTISSIRIIRYPSRLILIRVEGVSFLKRMVRNIVGTLVKVGTGGIQEDTVR
                     EFLLSQDRSNLPSSAPPQGLVFYKVIFDEFET"
     gene            complement(6721..7329)
                     /locus_tag="PLD15_02885"
     CDS             complement(6721..7329)
                     /locus_tag="PLD15_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62874.1"
                     /translation="MNHKYAHILYNDKEYLTPKKATFDHRTKAGFMTTWSTDHASLLL
                     KEKYWNCLSAFGLESAMRRKISFERKHSDTNLLYFKYELEVPDSLEGYFDATVVGAVS
                     RHLSIRETTEEVYKMLRDYADGSLKFNDQIISSWLGEKVSSLYLENREKSELENALLK
                     YVETIVSKILWNVYNGDLPRMGKDLSSMVYLYTEMLDLALSV"
     gene            complement(7326..7655)
                     /locus_tag="PLD15_02890"
     CDS             complement(7326..7655)
                     /locus_tag="PLD15_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62875.1"
                     /translation="MEDLVNKEYEGIEGKRRIDSIMTGLREKGVRPMKGRKLIEILDY
                     SPGIEGLPPSDVISIQFEGDLKLIARPSGTEPKIKFYLMARGSSREEARKNIDEMKEL
                     VEGIVVR"
     gene            complement(7640..8968)
                     /locus_tag="PLD15_02895"
     CDS             complement(7640..8968)
                     /locus_tag="PLD15_02895"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPB62876.1"
                     /translation="MRDNLAIEREYSRWLEHSSGELLGELESLSAGEVIERFAMDLEF
                     GTGGMRGKLGAGTNMMNSKTVARATLGFGKWILETGKPSVVIAYDTRNKSPEFAEVAA
                     DVLSSMGIRVYLFNEPTPTPVLSYAVRELKAGGGIVITASHNPSQYNGYKVYTSDGTQ
                     AVPEPAARITERVNESDYFEEYAPRKELIEIAPENILLGFMKTVENKVKSLCNNYENM
                     RLVYTPLHGTGNYPVYRVLSDLGHEVFRVEEQSTPDGSFPTVGYPNPEDPRAFEMALY
                     LARERNCDMIIATDPDCDRMGLMVKHNGEFLLLNGNQTGSIMAAFILEMMEGNLPDNP
                     FVVKTIVTTDLVNKIASDHGVAVKETLTGFKFIGELIEKSTVSGVGNFLFGFEESYGY
                     LFGKHARERTRSLRRLWRALSAGSSKRGAKRSTIISRGFTPGTGISWRIW"
     gene            complement(8974..9312)
                     /locus_tag="PLD15_02900"
     CDS             complement(8974..9312)
                     /locus_tag="PLD15_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPB62877.1"
                     /translation="MSRELGRQFEELACAYLKNTGYRIVARNVSYRFGELDIVAYDGR
                     VLVFVEVKGGNGFSPPRYRVDERKLRHLEMAANRFIAQERPKFDEIRLDVVEVLNTGE
                     INHIKSVGRW"
     gene            complement(9305..9610)
                     /gene="gatC"
                     /locus_tag="PLD15_02905"
     CDS             complement(9305..9610)
                     /gene="gatC"
                     /locus_tag="PLD15_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPB62878.1"
                     /translation="MNLETRVSAELLRHLESLSKLELEEGERTGLKRDIEEILQYMTL
                     LDDLDLSVDEMVSPVEIDLEPRPDEVQPFEHNEKITENFPEKSGPYITIPRIYKFDE"
     gene            complement(9607..10617)
                     /gene="galT"
                     /locus_tag="PLD15_02910"
     CDS             complement(9607..10617)
                     /gene="galT"
                     /locus_tag="PLD15_02910"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPB62879.1"
                     /translation="MPELRKDPIIRRWVIIATERARRPHDFINSKEKAETTFCPFDYG
                     NEHTTPPEVMAFRPADTEKDSPGWWVRVVQNKFPALDPGVEPERFGHGMYDVIKGFGG
                     HEVIVETPDHNATLATLSYQQVKEVIWAYKERFRSLEKDERIKYILIFKNHGREAGAS
                     LAHSHSQLIATPIVPKRVSEEIAGSIDYYSFRERCVYCDMVNQERLEGTRVVEENRDF
                     IAFEPFAARFPFETWIVPKVHNHNFGEITSGEIDGFAFILKNTLLRIHKSLDNPPYNF
                     MLHTGIRGGKDMTHYHWHLEIVPRLTRVAGFEWGSGFYINPMPPEHAAMYLREVKIDG
                     EG"
     gene            complement(10610..12019)
                     /locus_tag="PLD15_02915"
     CDS             complement(10610..12019)
                     /locus_tag="PLD15_02915"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HPB62880.1"
                     /translation="MRVLFVSYEVYPLAKVGGLADVAGSLPKALKELGLDIDVLMPFH
                     GSVQNRGIENTGRTVKTRFVKKEYEFELHRTKLPSSEVDLYLLKNDELMNAPEVYNVS
                     DLGLQAMAFSDAASVFASEGGYDLVHLNDWHTGLMAVYLDQIGKPIRTLISVHNLAYQ
                     GVYPENYLRISGIERDRWGMVSSGDNINCLKAGLIASDMINTVSPTYAREIQTSEYGA
                     GLDGVLRERKDDLIGILNGIDSTEYDPAKDRHLWVNFDSKNVEGKAINKGHLQDFVGL
                     PRTDNAVIGLISRLVDQKGLDLIAAIKEDLMELPLQLIVLGTGEKKYEGMFERLQVEC
                     PDKIAAKITFNLDLAQKIYGGCDMFLMPSRYEPCGLGQLFAMRYGTVPVVRLTGGLAD
                     TVKEFDPSSFEGNGFGFYDYSPEELLETLKKAIKAYEDQRTWSRIVSNAMEEDFSWKA
                     SARNYLAVYEKILEVKGDA"
     gene            complement(12024..12644)
                     /locus_tag="PLD15_02920"
     CDS             complement(12024..12644)
                     /locus_tag="PLD15_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB62881.1"
                     /translation="MDLHLLFPGSTLYVPGMMDASNSSCSLLIEDNRKVLVDPGGFPS
                     MKILEEKLSMFGLSPEDITDILLTHLHLDHAFNTIFFSRATVYLHESYSSRNYTSFGP
                     LVGKMYRTVIDSWNNVVTVSDGDTVLGGVKVLGSPYHSRDHVSYFLNSTNFGRVFICG
                     DVCIRQINYHEMRKGMRDDDAAAFVLKYFDEADTVIFSHDLPLFKR"
     gene            complement(12646..13434)
                     /locus_tag="PLD15_02925"
     CDS             complement(12646..13434)
                     /locus_tag="PLD15_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPB62882.1"
                     /translation="MSDFLKYIFLGFVQGATEFLPVSSSGHLTILSRFLNVPLNEESR
                     AAFFAILHLATFFAVLIFTYRDIWLILSGLADRKNRKNSLRYILLLLVATIPAVVFGF
                     LFEEKIEALFSDAWLASAMLFVTAFVLLISDMFKGKRRVLQLSIAGALSIGLFQAIAI
                     VPGISRSGMTIFGALLVGLARSESVRFSFLLSLPVTLGAGILKLSSVDLPLTTVLPAA
                     IVAFVTGLFGLWLVKLLVLKGRLRFFGIYCIIVGTVGLIILGVM"
     gene            complement(13427..>13708)
                     /locus_tag="PLD15_02930"
     CDS             complement(13427..>13708)
                     /locus_tag="PLD15_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPB62883.1"
                     /translation="ILEGLMEFTAMDKPGARISISLVDKPGALRDVVDFLADKEINIL
                     SIITASGDSEGTKRVVIRTDETDIQYIAGILEENGISFETISEEEGFGV"
BASE COUNT         2987 a         3790 c         3258 g         3673 t
ORIGIN      
        1 gattttgaat agattctatt ttgatgcatt aactctctct tgagtagatt taattttgat
       61 gcattacgac atttcgaaaa atttattcaa gagatttctc tatcgcagag aagaagagca
      121 actctttttc gatgaactct tctatatcgc catccatgac cgattgaatg tctcccgtct
      181 ctatttccgt cctgtgatct ttcaccatag tgtagggctg gaacacgtag gatcttatct
      241 ggttgcccca ggagatatct ttctgatcgc ccattagctt tagcttttcg ttcctctttt
      301 tctcgagttc gatttcgaaa agcttggcgt aaagcatttt catggcgttg gccttgttct
      361 ggtgttggga cctctccgtc tgacaggcga ccactatacc cgtaggcaga tgagttattc
      421 gaacggcgga atcggttttg ttaacatgtt gacctcccgc gccacccgag cggtaagtgt
      481 ctatcttgag ttcttcgggc cttatatcga tctccgggat ttcctccatt tcgggaaaga
      541 cgctgacgga cgcaaaagag gtgtgcctcc tgtggttggc gtcgaacggg gatatcctga
      601 ccaatcggtg aactcccgcc tcgaacttca acttcccata ggcgtaaggt ccggcaaagt
      661 tcagagtcac gctctttata ccggcctcgt ctccgggcag ttcatcgacg atcgtgactt
      721 tgtatctgtt cgcttcggcc catcttgtgt acatgcgcat cagcatcgag gcccagtcct
      781 gcgactcggt cccgccggct cccgggtgga tcgaaacaaa acagttgttg ttgtcgtaat
      841 ggccgctgag gagaatcgtg agttcgaatt ccctcacttt tctcgacgcc agggcgacca
      901 gttcctccac gtggcggaca tagctctcat cttccgaggc cagttcgacg gcgatatcta
      961 tattctcgaa ttcgccttcc acgtctttca acaattcaag ggtgcttctg aaagactggg
     1021 cttcctttcc aagattggag gcctcccttt tatcgttcca tatggacggg tcgctcattt
     1081 tatgctcgat tcccttcaac ttttcattca ttctcaccag atcaagcgtt ccctttatcg
     1141 agtcgaactt gtctctcaat tcctgaatct ttgagtttat ttcgtaactt atcaaattac
     1201 acctccgtac tagcgcttaa ccttgaacct tttggtgccc ttcttttttc cggtagaatc
     1261 ggatttctta tcggcggtac cgaactccga gtgtacggcc tgaaggttct tgaattcctt
     1321 gtcggctttt tgctgggcct tgtcgctatc gaccttcact attctgacga tggcgctcgc
     1381 tatatcgtcg taaagagaat ccgtcaattg ctggaaaagc tcgtaagatt cctttttgaa
     1441 ttctaccacg ggatctttct ggccgtaagc tctcaatccc accgaatcct taaggtggtc
     1501 tacggcttcc aggtgtctgc gccacctttc gtctatcatc ctcagcatta tgtatttcat
     1561 gacctggggg aactcctcgc cgaaggactt cttcttctcc tggtaagtat cttcgaggct
     1621 ttttatggcc gactctttca attcctggac ggttttcgac tcgctcaggc gggccagaga
     1681 atcgggcggc agaaaggcga aggagtttct gatctcctct ctggacggca tctcctgcaa
     1741 tccatcgagc cttctttcaa ctacgtcgcg aattatctca gagatgttcg aatccagttc
     1801 ttcgcccttc aaaacccagt ctctgtgggc gtaaatcgcg cttctctgct ggtccatgac
     1861 cgagtcgagc tcaaacagcc tttttctaat ttcgaagtgt atcccttcga tcttcttctg
     1921 tgaagaggat atgattctgc tgagcagcgg atgctcgatg ggctgtcccc tttcgatctt
     1981 cagggtcgtc attatcgatt gcattctctc cccgccgaac aacctgagca gatcgtcttc
     2041 cgtcgaaaga tagaacctgg attctccggg gtctccctgt ctgccagacc tgcccacgag
     2101 ctggttgtct atcctcctgc tctcatggcg ctcagttccg agcacgaaga ggcctccgag
     2161 atccacgact ccctcgccaa gcttgatgtc ggttcctcta ccggccatgt tcgtggctat
     2221 ggtgacggtt ttggcctgac cggccttggc gactatttcg gcttcgcgct cgtgatactt
     2281 cgcgttgaga acttcgtggg ccacgccttt cttctgaagt aaattgctca actgttcgct
     2341 cttctctatg gaggtggtcc ctaccagtac gggctgcccc ttttcgtatc tccttacgat
     2401 ctcctctatt atggcttcgt ccttttcttc acgggatttg tagatcagat cttccttgtc
     2461 ccctctcaca acttcgcggt tggtgggaac gactgccacc ggcgtgttgt acatggatat
     2521 gaattccgac tcttcggttg cggccgtccc ggtcattccc gagaccttct cgtacagttt
     2581 gaagtaattc tggaaagtga tcgtagcgaa agtgatcgat tcctgtctta tctgaacgtt
     2641 ttccttcgct tctatcgcct ggtgtaggcc ctccgagtat cttctgccct gaaggagtct
     2701 ccccgtgaac tcatccacta tcacgacctc tccctcttga ctcacgagat aatcgacctc
     2761 ttttctgtac agggtcatgg ccttgagggc gttgagcaga tggaagaggt agtcgtagtt
     2821 gctcggatcg tacaggttgt ctatctgcag caatttctcg gccttggcta taccttcatc
     2881 ggtgagggtg acggtcctgt ctttctcgtc gaccagaaag tccttttcct gttgaaacct
     2941 cttggcgaag aaggcgaact gccggtagag actggacgaa tcctcggccg gtccggagat
     3001 tatcaacggc gttctcgcct cgtctatcag tatcgagtcg gcctcgtcca cgataacgaa
     3061 gttgtggccg cgctgagctt tgttctcgag cgaatagacc aggttgtccc tgagatagtc
     3121 gaaaccgaac tcgttggccg tgccgtaggt aatgtcgcag ttgtaggctt ccttccgggt
     3181 gaccggatcc atgttggcct gtatgaagcc ggccttcatc ccgagatatt cgtacacggg
     3241 ccccatccag ccggcgtctc tcttcgccag gtagtcgttc acggttgcca ggtgacagcc
     3301 ctttcctgta agggcgttca gatacagcgg catggtcgcg acaagggtct ttccctcgcc
     3361 ggtcttcatc tcggctatct tgccttcgtt cagggccatg gctcccataa gctgcacgtc
     3421 gaaggccctc attcccgtgg tcctcttcgc cgcttccctc accagtgcga aggcctcggg
     3481 tatcaagtcc tccagaggac cgctttcggc cgctttcttc agctcttcgg ttcttttcaa
     3541 gaactcctct tgaggaagtt tactaaccga aggctccagg gagtttatct tatcgactac
     3601 cttcgaatac tttttaagaa gcattttgtt cttgtcgaaa atcttatcga aaatggacat
     3661 acaatcacct cagtaaaacc ggagagcgtg aaccatctta ccgttctcca gaaaggtttg
     3721 ctggtagaag atcgcactgt ccagcgtaat ccctatcagc gatttgaaga tcttcttcac
     3781 atcgacgaag gcctcgacga tgtctttctc cattcccagt tctcttacaa actcgtaact
     3841 gacgttgtcc cggagcgttc ccgacgatga aacggtcgag tagtactcct cgggagtgag
     3901 tgtcgtgagt accgtggtcg cgggtgagaa aatgaaatat atcgtgtcga aatcattctc
     3961 cagttcatat acccggtttc ccctgtaaat cgaagtcttt gcaccccact ctttgagggg
     4021 gctttcgagg aaggaggtat cgccgttcct gttcttgaaa acggccgaga cttttggaac
     4081 ctccgaaacc agctctccgt atatccgggc gttcccggtg ctctctctgg ataggagcac
     4141 tatgtaatcg gccagttccc tgtaaggttc gagatcgagc tcgaaccagt gggtcaactc
     4201 gtttctcaga gtttctacgg aagaggaggg gaagaagacc gtatagtcgc catgagtctt
     4261 ttccgaggcc tcgctctcct tcgtatcggc gagcttcaaa aaaacttcca gatcctgcga
     4321 attgaggaat atcgtgtcct gaactatggt ggccgataac gtatggcctt ccgggcgggc
     4381 gaaaacaacc gtgttgtcca ccgaaaacgg agattgaagt ccgagatcta tcttgaaacg
     4441 atcgctgtcg aagaagcccg tgatcgtgta gtcgtaactc atctctgcta tctctctccc
     4501 cttttcgcta gattcgagca accttctttc ctgtccctga taggtgttca gggccgtttg
     4561 aacggcttcc ctgttaccgc caattatcag aaaaccttct cctgtgtagt agaagagcgt
     4621 cccgctgttg ctcctcaact cgtttatgct ctcccccggc tgggcgaaga tcgtcatatc
     4681 cagcagggcg gcgacggctt tgagaagagt gaaggaagat tcgctcttcc agactatgaa
     4741 gatcctcgct ctggaaagct tgagcttctc cagaaggtaa aagggatcga acgtgagaag
     4801 atcttccagg gagagctcca ggccttctcc ggccagaaga aggtctctgt tcaaggcatc
     4861 cttcagcaga cttgtatcga cttcggcgtt gtaaccggtc gcgtctatta ttccgtaaag
     4921 agccctctcg gcaccgaaac cctcgtctcc aaggtaggca ccgaagaaat tggtactgtc
     4981 cctcagagtc tcgagagtcg aacgcatact tctcagaacg acaaaatacc ccgcactctc
     5041 gggtacgaaa tcgaaaagct cttgagccgc tactccagaa aaatggatca caaagcacaa
     5101 aagaaagacg aaaaatttca aaattaaccc gacgggccga attaaaagcg gatcgggtct
     5161 tccctccctt cgattgactg aaaactctcc cgattgctaa agagaaaaag ctctatcaac
     5221 tataaaccac gtatcgatat cgacgaagat ctgttttttc actgtcagga gcaaatactc
     5281 tatgttacca ttctttccac gaattggaga aaaagtcaag ttctgtgcat agagaccggc
     5341 ctctcagaag gcctttatgg atctctccaa aacttccacg tgaatggcgt gggacttcac
     5401 gacacctttg tggatcttac cgggaccggc ttcgaactgg ggtttcacca gggaaacgca
     5461 cagtccccca tccttaagaa gttcgctgat ttcgcgggcg accgaagaag tcggtatgaa
     5521 agagagatcg acagttacca gatctacgct ccccccgacc atctcgagag tgagttctct
     5581 cagatccctt tcttctaacg atacgacgcg ttcatcgttt ttcagcgatg ggtgaagctg
     5641 gttcttgccc acatcgacgg cgactacacg cctggcaccc ttctcaagca gcaactgtgt
     5701 gaaccctccc gtggaagagc cgagatctac aacctctttg cctgaaacgt cgaggccaaa
     5761 aaactccagg gccttttcca gtttgtagta tccgcgaccc acaggctgtt tcccgccggt
     5821 tatcgcgatg cggctttcga catcgaccct gaagccgggt ttttcgacga tcgagccctc
     5881 caccgacact ctgccggcct ttatcaattc gctggctttg ctcctactct ctgacattcc
     5941 cttaccgaat aaaagttcat cgagcctaag tttcgaactc atcgaatatg accttgtaaa
     6001 agaccagtcc ctgaggaggc gccgagcttg gaagattcga tctatcctga gacagaagga
     6061 actccctcac cgtgtcctct tgaatgccgc ccgttccaac ctttacgaga gtgccgacta
     6121 tgtttctcac cattcttttc aaaaaggaaa cgccctctac cctgatcaga atcaaacggg
     6181 aagggtacct gattatcctg atcgacgaga tggttctgtt ggggtttcta tcgtctctac
     6241 ccgtcttgaa agccgagaaa tcgtgctccc cggtcaggtg gccggcggcc ttcctcatcg
     6301 cctctatatc cagcccgtac gggaaccacc agacgtacct ccgccggaat atgtcgggtt
     6361 cgctcgaatt gagtatgtga tagtggtata tcctcttctt cgccgcaaat ctcggactga
     6421 aattggactc cacttcgtga acccttctga cgtaaatgtc ggccggaaga ttcgcgttca
     6481 gcgcgtcgca cattattttg gcgctcaaat gatcgaggct ggaatcgaag gctattacct
     6541 gtccgtaacc gtggacgccg gtgtcggtcc ttccggcgcc ctgagtcacg atcctgtatt
     6601 tgtggatcct ctcgagagcc tcttcgaagg taccctgaac cgtccgcatt tccggctggt
     6661 tctggaaacc gaaaaaatcc gtcccgtcgt aggagacaac agccgcaaat ctcttcatga
     6721 ctagacagac agggccagat ccagcatctc tgtgtaaaga taaaccatcg acgataagtc
     6781 tttacccatc cttggcagat ctccattgta aacgttccac agaattttgc tcacgatcgt
     6841 ttccacatac ttcaagagtg cgttttcgag ttcgctcttc tcgcggtttt caaggtaaag
     6901 cgaactgact ttttcgccca gccacgaaga aatgatctgg tcgttgaatt tcagcgaccc
     6961 gtcggcgtag tcgcggagca ttttgtaaac ctcttcggtg gtttccctta tcgaaagatg
     7021 cctggaaacg gccccgacga cggtcgcgtc gaagtatccc tccagtgagt cgggaacttc
     7081 cagttcgtat ttgaaataca ggagatttgt gtcgctgtgc tttctttcga aggatatctt
     7141 tcttctcatg gcgctctcca gaccgaaagc gctcagacag ttccagtact tctccttcag
     7201 aagcaaagag gcgtgatcgg tcgaccaggt ggtcatgaag ccggctttgg tccggtgatc
     7261 gaacgtagct ttcttgggag tgaggtactc tttgtcgttg tacagtatgt gtgcatattt
     7321 gtgattcatc ggactacaat cccttcaacc agttctttca tttcatctat attctttctt
     7381 gcttcttctc tgctcgatcc cctggccatg agatagaact tgatcttggg ctctgttccg
     7441 gagggccttg cgatgagttt gaggtcgccc tcgaactgta tcgatatcac atccgaaggc
     7501 ggcagcccct ctattcccgg gctgtaatcc agtatttcga tcaactttct ccctttcatg
     7561 ggccgaaccc ccttctccct caagcctgtc attatggaat ctatcctgcg cttaccctcg
     7621 attccttcgt actctttgtt caccagatcc tccacgaaat acccgtacct ggagtaaatc
     7681 ccctcgagat aatcgtagag cgtttcgccc ctctttttga ggaacccgcc gataacgctc
     7741 gccagagccg ccgcaacgac cgcgtccttt ccctggcgtg ttttccgaag agatatccat
     7801 aactctcctc gaaaccgaac aggaaattcc ccactcccga gactgtggat ttctctatca
     7861 actctcctat gaacttgaaa cccgtaaggg tttctttgac tgccactccg tgatctgagg
     7921 ctattttatt gaccagatcg gtcgtcacta tcgtcttcac tacgaaggga ttatccggca
     7981 ggtttccctc catcatctcg agtatgaagg ccgccatgat agagccggtc tgattgccgt
     8041 tcagaagaag gaactcgccg ttgtgcttaa ccatcaaacc catacgatcg cagtcgggat
     8101 cggtagctat tatcatgtcg cagttgcgtt cacgcgccaa atacagggcc atttcgaagg
     8161 cacgcggatc ctcgggattg ggatagccga ccgttggaaa actaccgtcg ggcgtcgact
     8221 gttcctcgac gcggaagact tcgtggccga gatcagacag cactctgtac accggatagt
     8281 tgccggtacc atgtagagga gtgtatacca gacgcatatt ctcataattg ttgcacaaag
     8341 atttcacttt attttcaacg gttttcatga aacctaaaag tatattttcc ggagcgatct
     8401 cgataagttc cttcctgggg gcatactctt cgaagtaatc cgactcgttt accctctcgg
     8461 ttatcctcgc ggcaggttcg ggaacggcct gcgttccatc ggaagtgtag actttgtatc
     8521 cgttgtactg agaagggttg tggctcgccg tgatcacaat gcccccgccc gctttgagtt
     8581 cccttacggc gtaactgaga acgggagtcg gtgtcggttc gttgaagagg taaaccctta
     8641 ttcccatcga agatagtaca tcggctgcca cttcagcgaa ctccggcgat ttgttcctcg
     8701 tatcgtaggc tatgacgacc gatggtttgc ctgtttcgag tatccacttt ccaaaaccca
     8761 gcgtagccct cgcaacggtc ttcgagttca tcatgttggt cccggcgccg agcttgcccc
     8821 tcattccgcc ggtaccgaat tccagatcca tggcgaaccg ctcgatcacc tctccggccg
     8881 agaggctttc aagttcgccg agcaactcac cggaagaatg ttcaagccat ctactgtact
     8941 ccctttctat cgccagatta tctctcatac ttcctaccac cttccgacac tttttatatg
     9001 atttatctct cctgtgttca gcacttcaac cacatcgagg cggatttcgt cgaatttcgg
     9061 cctttcctgc gctatgaacc tgttggccgc catctcgaga tgtctcaact tcctctcgtc
     9121 caccctgtac ctgggcgggg agaagccatt tccgcctttg acttcaacga aaaccaggac
     9181 tctgccatcg taggccacta tatcgagttc tccgaacctg taggaaacgt tcctggcgac
     9241 tatcctgtat cccgtgtttt tcaggtaggc gcaggcgagc tcttcgaact gccttccaag
     9301 ttccctactc atcaaattta tagattctgg gaatggttat gtacggtccg gacttctccg
     9361 ggaaattctc agttatcttt tcgttgtgtt cgaagggttg aacctcgtcg ggtctgggct
     9421 cgagatctat ctccaccgga gataccatct cgtccacgct caggtcgagg tcatcgagca
     9481 gagtcatgta ctgtagaatc tcttcgatat ccctcttgag accggttctt tctccttctt
     9541 ccagctcgag tttcgaaagg ctctctagat gccgtaaaag ctccgcgcta actctggtct
     9601 ccaagttcat ccctccccgt cgatcttgac ttctctcaaa tacatcgccg catgttccgg
     9661 gggcattgga tttatataaa aacccgagcc ccactcgaat cccgccaccc tcgtaaggcg
     9721 cggcactatc tcaagatgcc agtgatagtg agtcatatcc ttaccgcccc tgatacccgt
     9781 gtgcagcatg aagttgtaag gcgggttgtc gagggactta tgtattctga gcaacgtgtt
     9841 cttcaggatg aaggcgaaac cgtctatctc tccggacgtt atttcgccga aattgtggtt
     9901 gtggactttc ggaactatcc aggtctcgaa gggaaatctg gcggcgaagg gttcaaaggc
     9961 tatgaaatct ctgttctctt cgacgaccct ggttccttcg agccgttcct gattcaccat
    10021 atcgcagtag acacacctct ccctgaagct gtaataatct atcgatccgg cgatctcttc
    10081 gctgactcgc ttgggcacta tgggcgtggc tatcagctgg ctgtgcgaat gcgccagaga
    10141 agcgccggcc tctcttccgt ggtttttgaa gatcagaatg tacttgatcc tctcgtcttt
    10201 ctcgagagat ctgaaacgct ctttgtaagc ccagattact tctttcacct gctggtagga
    10261 gagcgttgcc agcgtggcgt tgtgatcggg cgtctcgacg atgacctcgt ggcctccaaa
    10321 accctttatc acatcgtaca taccgtgacc gaatctctcc ggctccacac cgggatcgag
    10381 ggccgggaac ttgttctgga cgactctgac ccaccagccc gggctgtcct tctcggtatc
    10441 ggccggcctg aaggccatga cctccggcgg agtcgtgtgt tcattgccgt aatcgaaggg
    10501 acagaaggtg gtttcggctt tttctttgga gtttatgaaa tcgtggggcc ttctcgccct
    10561 ctccgtggct attatcaccc agcgtcttat tatcggatct tttctgagtt caggcatcgc
    10621 ctttgacctc cagaatcttc tcgtaaactg ccagatagtt cctggccgag gctttccagg
    10681 agaagtcttc ctccatagcg ttggagacga tcctgctcca ggtccgctga tcttcgtaag
    10741 cctttatagc ctttttcaga gtctcgagaa gctcttcggg actgtaatcg tagaatccaa
    10801 agccgtttcc ctcgaaactc gatggatcga actccttcac cgtatcggcg agaccaccgg
    10861 tgagacgtac cacgggcacc gttccgtatc tcatggcgaa aagctggccg agcccgcagg
    10921 gttcgtatct cgagggcatc aggaacatgt cgcagccacc gtagatcttc tgggccaggt
    10981 ccaggttgaa ggttatcttc gccgcaattt tatcggggca ctcaacctgc agtctttcaa
    11041 acatcccttc gtacttcttc tcgcccgtac ccagcactat tagctggaga ggcagttcca
    11101 tcaaatcctc tttgatcgcg gctatcaggt ccagtccctt ctggtcgacg agtcttgata
    11161 taaggcctat cacggcgttg tccgtgcgcg gcaggccgac aaaatcctgc aaatgccctt
    11221 tgttgatagc tttgccttcg acatttttag aatcgaagtt gacccagaga tgtctgtcct
    11281 tcgccgggtc gtactcggtt gaatctatgc cgttcaaaat accgatcaga tcgtcttttc
    11341 tctctctcaa caccccgtcg agcccggcgc cgtactcgct tgtctgtatc tctctggcgt
    11401 aggtcggcga taccgtgttg atcatatcgg aagctatcag cccggccttg aggcagttga
    11461 tgttgtcgcc cgaggagacc atcccccacc tgtctctctc tatccccgag atgcgtagat
    11521 agttttcggg gtacactccc tggtaagcca gattgtgtac ggatatcagt gttctaatcg
    11581 gctttccgat ctggtcgaga tagacggcca tcagtccggt gtgccagtcg ttcagatgca
    11641 cgagatcgta accgccttct gaggcgaaca cggaagcggc gtcggaaaag gccatggcct
    11701 gaagtccaag gtcgctcacg ttgtaaactt ccggagcgtt catgagttcg tcgttcttga
    11761 gaaggtaaag gtccacctcg ctggagggta atttagttct gtgaagctcg aattcgtatt
    11821 cctttttgac gaacctggtt tttacagtcc tgccggtgtt ctctattcct ctattctgta
    11881 ccgagccgtg aaaaggcatc agcacgtcta tatccagtcc cagttccttc aaggctttgg
    11941 gaagagatcc cgccacatcg gcgagtcctc ccacttttgc cagaggataa acttcatacg
    12001 aaacgaaaag tactctcata cattcacctc ttgaaaagcg gcaaatcgtg agagaagata
    12061 accgtgtcgg cttcgtcgaa atacttcaaa acgaaggccg ccgcgtcgtc gtctctcatt
    12121 cccttgcgca tttcatgata atttatctgc cttatgcaga cgtctccaca gatgaaaacc
    12181 cttccaaagt tggtcgaatt caagaagtac gatacgtggt ccctggaatg atagggcgaa
    12241 ccgaggacct tcacgcctcc gaggaccgtg tccccgtcgg aaacggtaac cacgttattc
    12301 caggaatcaa tgaccgttcg gtacatcttc ccgaccagcg gtccgaagga ggtgtaattc
    12361 ctgctggagt agctctcgtg gagatagaca gttgcgcgag aaaagaaaat cgtgttgaac
    12421 gcgtggtcca gatgtagatg cgtgagaagg atatcggtta tgtcttctgg agaaaggccg
    12481 aacatcgata gcttttcctc gaggatcttc attgagggaa atccgccggg atcgaccagc
    12541 acttttctat tatcttctat aagtaatgaa cagctactat ttgaggcatc catcattccc
    12601 ggtacataga gggtgctacc gggaaagagg agatgtaagt ccaaatcaca tcactcccag
    12661 aataatcaaa cccaccgtac caacaattat acagtaaata ccgaagaatc tcagtcttcc
    12721 cttgagcacg agcagtttca ccaaccacag gccgaacaac ccggtaacga aggcgactat
    12781 cgccgccggc aagaccgtcg tcagaggaag gtcgacgctg gacagtttga gaataccggc
    12841 ccccagagtg acgggcaggc tcaacaggaa agagaacctc accgactcgc ttctggccag
    12901 accgacgagc aaagcgccga agatagtcat gccgctcctg gagatgcccg gtacgatcgc
    12961 tatggcctgg aaaagtccta tcgaaagcgc gcccgctatc gagagctgca aaaccctcct
    13021 ctttcccttg aacatatctg agataagcaa tacgaaagcc gtcacgaaca gcatcgccga
    13081 ggcgagccac gcgtccgaga agagagcctc tatcttttct tcgaagagga aaccgaaaac
    13141 cacggccgga atcgtggcca ccagaagcag gagtatgtat ctgagcgagt tctttctgtt
    13201 ttttctgtcg gccaatcccg agaggatcaa ccagatgtcg cggtacgtga aaatcaaaac
    13261 ggcaaagaaa gtggccaggt gaagaatcgc gaagaaagcg gccctcgatt cttcattcag
    13321 cgggacattc aaaaaacgcg agaggatcgt cagatgaccc gatgaagaaa cggggagaaa
    13381 ctcggtcgcg ccctgcacga atccaagaaa tatgtatttg agaaaatcag acaccgaaac
    13441 cctcctcttc gcttatcgtc tcgaaggata tgccgttctc ctcgagtatc ccggctatgt
    13501 actgaatatc cgtctcgtcc gtccttatca ctaccctttt ggttccctcg ctgtcgccgg
    13561 aggcagtgat tatcgagagg atgttgatct ccttatcggc cagaaagtcc accacgtccc
    13621 taagcgcgcc gggcttatcc accaggctga tcgagatcct cgcgccgggt ttgtccatgg
    13681 cggtgaactc catgaggcct tcgagaat
//
LOCUS       DAOOLY010000033        13596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4953_length_13596_cov_5.047855, whole genome shotgun sequence.
ACCESSION   DAOOLY010000033 DAOOLY010000000
VERSION     DAOOLY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13596
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_13596_cov_5.047855"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLD15_02935"
     CDS             <1..491
                     /locus_tag="PLD15_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62884.1"
                     /translation="GIFVDQEGNTMDQDGVMIVSEWVKSINSPYPELYWVTDTWYPNS
                     WEFRFEGEGVPENIRHFFLKPVVEGGQSGFFAHGPQYSEGGVYALDAEGNIIGKGFAE
                     SVSYADTAENALAITGLPYNEEIRQALESPSISDFDKLIIGIYLAWPENQEKLIKLVS
                     DM"
     gene            complement(850..1695)
                     /locus_tag="PLD15_02940"
     CDS             complement(850..1695)
                     /locus_tag="PLD15_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPB62885.1"
                     /translation="MPLATLVEVLEDARKNSYAVPGFNFHLYEDLTAIVEAAREVRSP
                     VILMAAGTCMKHWGPTLAATLIREVANKVDIPVVAHLDHASSMDLIFKSMHAGFTSVM
                     YDGSMLPVEENIANSKIVVEVAKAFGVSVEAELGRVAKGEEGEAAREILTDPSDVVMF
                     CERTGVDALAVAVGTVHGMQKQEAKIHLDLVDALSRVAPVSLVLHGSSGVSDEDLRYI
                     AKTAFSKINIGTRIKTVFTESIRETLRVRPDLKDQLQLLQLSAPKIKETVKEKIRLLG
                     SENRC"
     gene            complement(1698..3155)
                     /locus_tag="PLD15_02945"
     CDS             complement(1698..3155)
                     /locus_tag="PLD15_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB62886.1"
                     /translation="MKAYLGIDTGTTNMKCLVLGSNGNILEVMHEETPKKRIAGADYL
                     DLEATRSIVDKFVSEASCQYSLGGMAFSSIGETVVPAYKGKALSDPLMWYDGATRHIW
                     EKNRTAVDGFSPYRITGVENEYTFSIYKILFQRETLQSDAVEHWLPVASYFAYSLGAR
                     PTWDMSLACRSFMIDIHRRCWNSPLLEYMGESPERMGELVYTGRPIGLTADGIPIVSA
                     GHDHITGLFAARVFARGEEFLFDSMGSASVVAAVIKTGDRNLVFDSPFMSGGTVGIAF
                     EDSQYYIESNLRYYGKLLQSLMNLTGLQASKEGYEKINSEIEKGKTWHSEPLFLVNGD
                     LSAGEGMHGITVLEMPTTLTREQLIQSAYIYLASSSKLIVDNLEKITGRKLPVICGGG
                     GSLNGLLMKYKASLLEREIWVLPTSELTALGGALAAASGVGDTRTVDRCVENLRPLKT
                     EVDHEIAARLMEIFNKNSSRYSTIERERKIKFQEG"
     gene            complement(3152..4075)
                     /locus_tag="PLD15_02950"
     CDS             complement(3152..4075)
                     /locus_tag="PLD15_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB62887.1"
                     /translation="MNLNPCYDHWVILKKPPKIPNVVRGDEVVSLVDGKGLNIARVLS
                     RVFSFDEYFCINILGGQVGKIIEKECNDLGIKTDNFWIEDSNRINTALVYEYENRMLM
                     INEPGPVMKIGEVRAFIEYFKERIGSGVNLVISGSAPRGFENGEMIDLVIAARNSGCT
                     LKIDIAGAWLSKIVALSPELLKINADELKVAFGIEKGDLKNMEEFRRGSSIKDLIITN
                     GKHGSVWLSDTEKFRAVSTKVFSDFSVGSGDSFFAGLIYGQERLASKREALKMATACG
                     AANTLHYGAAIFERSDVERVIKDVVVSDVEL"
     gene            complement(4092..5060)
                     /locus_tag="PLD15_02955"
     CDS             complement(4092..5060)
                     /locus_tag="PLD15_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HPB62888.1"
                     /translation="MISDELLFEVASDYYVRHMLQKDIALKYGVSRVQISKYLKMAEE
                     RGIVRIEVEPPSIKSSVIKEYEAFFTSRFGLKRLFIARGARNEKTVLRALGREVAKYL
                     GTLPEREMNIGLGWGNTIFTVAETVERLDRPRWQIIPLSGGTARLADKRFNINHIVQN
                     FASRISARAVPMYLPFILENARQLENTKQSLEYMNIQKLWERLDVIICSVGYSIARSP
                     LFRENLLDVSYADELEKRSVVGDVLTHYFDINGKMFEKNILENCINLGFEQYLKAGER
                     VIVAAGHHKVDGIVGALRGGLIDTLITDEFTARFVKEYVLYDEGGE"
     gene            complement(5064..5900)
                     /locus_tag="PLD15_02960"
     CDS             complement(5064..5900)
                     /locus_tag="PLD15_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB62889.1"
                     /translation="MTRSQKRKLKKTITYILLIIISVVVAFPFLWLVLTALKTYPDIY
                     AYPIKYFIFEPTREHFEKISSMNFWSYFKNSVIVGAGTMFFSILIGLFPAYAFARYEF
                     KWKRTLLTGVLIFQMFPQVVFLLPIFKALNTTGLINTYTGLILSYLPFTTPISIVFMR
                     TFFLSIPKSLEEAARIDGCNFGQAFRKVILPITIPGIAAVGIYAFLFSWSELLYSMSI
                     LTSKAKQTIPTFLQLFVGQYQTRWGPLFAGSILATLPPLIIFMILQKFFIAGLVSGSV
                     KE"
     gene            complement(5897..6763)
                     /locus_tag="PLD15_02965"
     CDS             complement(5897..6763)
                     /locus_tag="PLD15_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62890.1"
                     /translation="MSTLNREEKSALKMVLPYVILTVLLFVFLLGSNLFNSFTDKNGN
                     WTLQNYIDNFTDPVVGRTLLNTIVWVVGSVSGQLLLGLFVALLLNESTKGQIIFRSII
                     LILPWAVLDIVAGVMWKWMYNDMYGVLNDVLVKMHIIRDYIPWLATENMAMLSVIIAN
                     IWKGFCLSGMFFLAGLQTIPIDLYEAAEIDGANSFKRFWTITVPQLKPVIMTTLMLTT
                     IWTINYFPLIYTMTGGGPGYGTETIVTYIYKLGFKFMEFNRSASLSNVLFVIIFFIAF
                     LFLRSIAKEEAR"
     gene            complement(6837..8078)
                     /locus_tag="PLD15_02970"
     CDS             complement(6837..8078)
                     /locus_tag="PLD15_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB62891.1"
                     /translation="MRRSSLLVLLMLVVLAIAGVSQTINIWIGGQVAELDETWDMIIG
                     KFEKETGQKVEVQLFGFDIYYDKLLTALQAGQGPDLAFADLGGWVPTFAEKGWLEPMG
                     ALLDSWDGVEQIWPNLWPTVIYNGERYGLPWYTDCRLLLYNQTMFDSAGLDRNDPPET
                     WEELVYQATKLTDASKRVYGYGVSGTKTEHTSLAFIIFLYGAGGQLLTDDYSAAAFNT
                     PEGLRALKFYTDLALTYGVSPNAVTYNEDDYRNMMAQNRVAMAIGGPWSFPLIETANP
                     AIVNNYTVSLHPYGSTPASVLGGWALVIPSVSKNKENAWELAKYLTSFETWMTWIEAK
                     GGPMPTRKDVCYAAPVLQDSKWQVIFETFPFAVSRPPIPQYPQVSEQIQIMIQNVLLG
                     NATPEEAIKTAEANVNAILAK"
     gene            8579..10195
                     /locus_tag="PLD15_02975"
     CDS             8579..10195
                     /locus_tag="PLD15_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPB62892.1"
                     /translation="MNPGKAKLLKNLLVGATGGILTFSTGLAANYEDVMTKETVKPAP
                     NLEEVIPHPEQMQEALAKLAELESRTGKKPNILIYLMDDVGWGDLGVYGGGVMVGAPT
                     PNMDALAREGLMLTSAYSQPSSSPTRATLLTGQLPVRHGILRPSLYNEPGGLGDSITL
                     AELLSEAGYVTQAVGKWHLGENLASQPQNVGFDHFTGFLSVSDMYTEWRDIDFYPEIA
                     LSEGRTQMMRDFAFEKYWVQASKNGELERIAEIDIDVLTYLDQNWADFSVNFIKEMAD
                     SDQPFFLYHGTRGAHFDNYPPEEYLGKSPAKQRYKDTIVELDDILGRLVKALEETGQL
                     ENTLIFIASDNGPEMEAWPDSAYSPFRGAKGTTWEGGVRVPGILYWKGMIEPGRVSDG
                     LFDLADIFNTSLTLAGAQDLMPTDRYIDGIDQTSFLLAKDGLSNRKYIYYWLMNYFSA
                     VRMAEYKYMTLAEADDEPAGVYRAGFTGSLDMFAYYHMYNLYLDPKEEHNVSIRKLVY
                     TDLFLAAIHKHFATLKQYPPAVEVFLGLVR"
     gene            complement(10316..10585)
                     /locus_tag="PLD15_02980"
     CDS             complement(10316..10585)
                     /locus_tag="PLD15_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62893.1"
                     /translation="MKFNEKTIMAIMVDNRKEEAVQVQKVLTGWGCLIKTRLGIHDGV
                     LDSCSNYGLIILELVGEREKHEELMNKLNVLPGVQAKYLSLELEE"
     gene            complement(10596..11699)
                     /gene="nagA"
                     /locus_tag="PLD15_02985"
     CDS             complement(10596..11699)
                     /gene="nagA"
                     /locus_tag="PLD15_02985"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPB62894.1"
                     /translation="MELKNVLVVDPVDGEYTANVRISGNKIESVDRTHGNPRLIMMPG
                     FVDTHSHGAAGVNSMKMDHIGLEKWEEFLYPHGVTFLLPSTVSALKKDMLRVANLVSS
                     YMEEKARTSIRGVHYEGPYINVKKKGAQNPETIRPATPKELREILTDDVILVTMAPEI
                     DGFFEALAEMKMQDVVVSMGHTDATFAQINKAFEAGCDRMTHFPNGMNTLHHREVGCV
                     GAGLMLPLKLEMIVDGFHTAPEFVRLVYVIRGTDNIILVTDSIDATGLEDGIYDLGGL
                     RVTVNEGKATLDDGTIAGSTLVLDQAVRNFRKWTGCSLVELARVSSYNALTNLGVRNR
                     GRIKEGYLADLVIMDSELNVVETILAGKSVYKS"
     gene            complement(11739..12230)
                     /locus_tag="PLD15_02990"
     CDS             complement(11739..12230)
                     /locus_tag="PLD15_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPB62895.1"
                     /translation="MRSILRVAGPLLIFIGVVILIIAMVDFFSAFGSFGQPNKFYLFF
                     LAIPFIFLGAIATSYGFMGAVARYKANELAPVAKDTINYMAEGTKDGVKTIARSIKEG
                     ISGSEESKVVCQKCGTGNAPDSRFCKNCGATLRKDKKCPDCGEQNDSDARFCDNCGFK
                     FPV"
BASE COUNT         3295 a         3537 c         3165 g         3599 t
ORIGIN      
        1 acggcatatt cgtcgatcag gaaggcaata cgatggatca ggatggagtt atgatcgttt
       61 ccgaatgggt gaaatcgatt aattcgcctt atccagaact ttactgggta acggatacat
      121 ggtatccaaa ctcctgggag ttccggtttg agggagaggg agttcccgaa aatattagac
      181 acttcttcct taagccagta gttgagggcg gacaatctgg attctttgct cacggaccac
      241 aatactctga aggtggagtt tacgcattgg atgccgaggg aaatataatc ggaaagggtt
      301 ttgccgaatc ggtatcgtat gccgacactg ccgaaaacgc gcttgccata accggactcc
      361 cgtataatga agaaatcagg caggcactgg agtcaccatc tatctcggat ttcgacaaat
      421 tgatcattgg tatttacctc gcatggccgg aaaaccagga gaaattgata aagttggttt
      481 ctgatatgta atgaaagatg gtcgggcaca agaggatttg acgcaagttc agacgacagg
      541 tgaataaatg cacaaaactg gaaggcgggc gactttggtc gttcgccttc cagtacttat
      601 tcaaagacat gaaaaagaac gtattcgaga ggttcccttc gctcacgaga aggcgagaat
      661 tctctaggag ccgttgtgcg ttgtagttaa gagcacgtga ttctgagtca agctcagatg
      721 acagattgat gtgctccgcg cgcgctttat gtccatgctc tgggacggac aacggatgac
      781 gaagaacggc ttccaacgct ctttccagag acggctctta attctctcca cgactgatag
      841 ctgaacttct cagcatctat tttcgctgcc gagcagtctg atcttttcct taacggtttc
      901 cttgattttc ggcgccgaaa gctgaaggag ctggagctgg tccttcaggt ccggcctgac
      961 gcggagcgtc tctcttatgc tttcggtgaa gacggttttg attctcgtgc cgatgttgat
     1021 tttggagaag gccgttttgg cgatgtatct gagatcttcg tccgacacgc cgctcgatcc
     1081 gtgaaggacc agcgatacgg gagcgactct cgagagagcg tccacgaggt ccagatgaat
     1141 cttcgcttcc tgtttctgca taccatgaac ggtccccacg gccacggcga gagcgtccac
     1201 ccccgtcctt tcgcagaaca tgacgacatc cgacgggtct gtgaggattt ctctggcggc
     1261 ctccccttct tcgcccttgg cgacccttcc gagctctgcc tcgactgaga ctccgaaggc
     1321 tttggcgacc tctactacga ttttgctgtt ggctatgttt tcttcgaccg gcagcatcga
     1381 accgtcgtac atgaccgagg tgaaaccggc atgcatagac ttgaatatca ggtccatact
     1441 gctggcgtga tcgagatggg ccacgaccgg aatgtcgact ttattagcta cctctctgat
     1501 caaagtcgcc gcgagtgtcg gcccccagtg cttcatgcag gtgcctgccg ccatgagtat
     1561 tacaggagag cgaacttccc gggcggcctc gactatcgcg gtcagatctt cgtaaaggtg
     1621 gaagttgaaa cccggaacgg cgtagctgtt tttccttgca tcttcaagta cttctacgag
     1681 tgtggcaagt ggcatttcta cccctcctga aattttatct ttctttctct ttctatggtt
     1741 gagtatctgc ttgagttctt gttgaatatc tccatcagtc tggcggcgat ctcatgatcg
     1801 acttccgtct tcaacggcct caggttttcc acacacctat cgacggtccg ggtatctcca
     1861 acgccgctgg ccgccgcgag ggctccacca agagccgtca attcgctcgt gggcaaaacc
     1921 cagatctctc tttccagaag agaagctttg tacttcatca gcaggccgtt caggctcccg
     1981 cctccgccgc agataaccgg cagcttcctt ccggttatct tctcgaggtt atctactatc
     2041 aacttgctgg aagaggcgag atagatatag gcagactgga tcaactgctc ccgggtgagc
     2101 gtggtgggca tctcgagaac ggtgatgccg tgcataccct ctcccgcgct aagatcaccg
     2161 ttcacgagaa atagcggttc ggaatgccag gttttgccct tctcgatctc cgagttaatt
     2221 ttttcataac cctctttgct tgcctggagg ccggtgagat tcatcaagga ctggaggagt
     2281 ttcccgtaat aacgtaggtt gctttctata taatactggg aatcttcgaa ggctatcccg
     2341 acagtcccgc ccgacatgaa gggactatcg aagacgaggt ttcgatctcc ggtctttatc
     2401 acggccgcca ctaccgaagc cgaacccatg gaatcgaaga gaaactcctc gcctctggcg
     2461 aataccctgg ccgcgaagag gccggttatg tgatcgtgtc cggcgctcac tatcgggatg
     2521 ccgtccgcgg ttaaccctat cggtcggccg gtatagacca gctcgcccat tctctcggga
     2581 gattctccca tatactctag tagcggagag ttccagcagc gccggtgtat atctatcatg
     2641 aaggaacggc aggccagact catatcccag gtcggcctcg caccgagcga gtaagcgaaa
     2701 taagaggcca ccgggagcca gtgttcgacg gcatcggatt gcagtgtttc tctctggaac
     2761 aggatcttgt agatggagaa ggtatattcg ttctccacgc cagtgatcct gtaaggggaa
     2821 aaaccgtcaa cggccgttct gttcttttcc cagatatgtc ttgtcgcacc gtcgtaccac
     2881 atcaggggat ctgaaagcgc tttcccttta tacgcaggaa cgaccgtctc gcctatactg
     2941 gagaaggcca taccgcccaa tgagtactgg caagacgctt cggatacgaa tttgtctaca
     3001 atagagcgcg tcgcttcgag atcgagataa tccgctcccg caatcctctt ttttggagtc
     3061 tcctcgtgca tcacttcgag aatatttccg ttcgacccca gcaccaggca tttcatattc
     3121 gtcgtgcctg tatctatccc cagatacgcc ttcatagttc cacgtccgaa actacaacgt
     3181 ctttaattac cctctctaca tcggagcgct caaatatggc cgcaccataa tgcagtgtgt
     3241 tggccgcacc gcaggctgtc gccatcttca gggcctccct ctttgaggcc agtctctcct
     3301 ggccgtagat caacccggcg aagaaggaat cgcccgagcc gactgaaaaa tccgaaaaga
     3361 ccttcgtcga aacggcccga aatttttcgg tgtctgaaag ccatacgctt ccatgcttcc
     3421 cgttggttat tattaaatct ttgatcgaag agcccctcct gaactcctcc atgttcttca
     3481 aatcgccctt ctcgattccg aaggccactt tgagttcatc ggcgtttatt ttgagaagct
     3541 ccggcgacag tgcgacgatt tttgagagcc acgccccggc gatatcgatc ttgagcgtgc
     3601 aaccgctgtt ccgggccgct ataaccagat cgatcatctc tccattttca aatccccgcg
     3661 gtgcactccc cgaaataacc aggttcacgc ctgacccgat tctctctttg aaatactcta
     3721 taaaggctct gacctctccg atcttcatca caggacccgg ctcgttgatc atgagcattc
     3781 tgttctcgta ttcatagacc agggccgtat tgattcgatt gctgtcttcg atccagaaat
     3841 tatcggtctt gatgcccaga tcgttgcact ccttctctat tatcttgccg acctggccac
     3901 cgagaatatt tatgcagaaa tactcgtcga aactaaaaac cctcgaaaga acccgggcga
     3961 tgttgaggcc cttgccatcg acgagcgaaa ccacctcgtc ccccctcaca acgttcggaa
     4021 tctttggcgg ctttttgaga ataacccagt gatcgtaaca gggattaaga ttcaagatac
     4081 aggtattgat gttattctcc cccttcgtcg tacaacacgt attccttgac aaacctggcc
     4141 gtgaattcgt cggtgatcag agtgtcgatc aaacctcccc tcagcgcccc gactatgccg
     4201 tcaaccttgt ggtgaccggc cgcgactatt accctttctc cggccttcag gtactgctcg
     4261 aaaccgaggt ttatgcagtt ctcgagtatg tttttctcga acatcttgcc gttaatatcg
     4321 aagtaatggg taagcacatc tccgacgacg cttctcttct ccagttcgtc ggcgtagctc
     4381 acgtcaagaa gattctctct gaacagcgga gaacgggcta tagaatagcc aacgctacag
     4441 attatcacat ccaggcgttc ccagagcttc tgaatattca tgtactccag agattgcttc
     4501 gtgttttcca gttgccgggc gttctccaga atgaatggga ggtacatcgg cacggctctg
     4561 gccgatatcc tgctggcgaa gttctgtact atgtggttta tattgaatct tttgtctgcc
     4621 agcctcgccg ttccaccgga cagaggtatt atctgccacc ttggacgatc cagtctttcc
     4681 accgtctctg cgaccgtgaa aattgtgttg ccccatccca ggccgatatt catctctctt
     4741 tccggcagag tcccgaggta tttcgcgact tcccgtccga gcgccctcag cacggtcttc
     4801 tcgttccgcg ccccccgggc tatgaaaagt ctcttgagtc cgaatctgga agtgaagaag
     4861 gcttcgtact cctttataac ggaggatttt atcgaaggcg gttctacttc gatacggact
     4921 atacccctct cttcggccat ctttagatac ttgcttatct gaaccctaga tacgccgtat
     4981 ttgagggcta tgtccttctg gagcatatgt ctcacatagt aatcgctcgc aacttcgaac
     5041 aataactcgt cacttatcat cttttactcc ttcaccgagc cgctaaccag tccggcaatg
     5101 aagaacttct gaagtatcat aaatattata agcggtggca gtgtcgcgag aatcgaaccg
     5161 gcgaagagcg gtccccacct ggtctggtat tgccccacga aaagctggag gaaggtgggg
     5221 atcgtctgtt tcgccttact ggtcagtatg gacatcgagt aaagcaactc gctccatgaa
     5281 aagagaaagg cgtatatacc cacggcggcg ataccgggta tggttatggg tagaatcacc
     5341 ttcctgaagg cctgcccgaa gttgcatcca tctatcctgg ccgcctcttc gagcgatttg
     5401 gggatcgaaa ggaaaaaggt tctcatgaac acgatcgaga tcggcgtcgt aaagggcagg
     5461 tacgacagaa tcaggcccgt gtaagtgttt atgagccccg tggtgttgag cgctttgaag
     5521 atcggcaaca ggaagaccac ctgcgggaac atctggaata tgaggacacc ggtcagaaga
     5581 gtcctcttcc atttgaactc atatctggca aaagcgtacg ccgggaagag ccctatgagt
     5641 atggagaaaa acatcgttcc ggcacccacg ataacgctgt tcttgaagta agaccagaaa
     5701 ttcatgctgg agatcttctc gaagtgttcc ctcgtcggtt cgaaaatgaa gtactttatc
     5761 ggataggcgt agatatccgg ataggttttt aaggcagtta gaaccagcca cagaaaggga
     5821 aaagctacca ccaccgagat aattatcagc agtatgtatg tgatagtctt ttttagtttt
     5881 ctcttctgac tcctcgtcat ctcgcttcct ccttcgctat gcttctaagg aatagaaagg
     5941 cgatgaagaa gattatcacg aacagcacgt tggaaagcga ggccgacctg ttgaattcca
     6001 tgaacttgaa acccaattta tagatataag tgacgatagt ctctgttccg taccccggtc
     6061 cgcctccggt catggtgtag atgaggggaa agtaatttat cgtccagatt gtagtcagca
     6121 tgagagtcgt cataataacc ggtttcaatt gaggaacggt gatcgtccag aatctcttga
     6181 aggaattggc cccgtcgatt tcggccgctt cgtaaaggtc gatcggtatc gtttgaaggc
     6241 ctgccaggaa gaacatgccc gaaaggcaga agcccttcca tatgttggcg ataataaccg
     6301 agagcatcgc catattctcc gtcgcgagcc agggtatata gtctcttatt atatgcatct
     6361 tgaccagaac atcgttcagc acgccgtaca tatcgttgta catccatttc cacatgactc
     6421 ccgccactat gtcaagcacg gcccatggaa gtatgagtat gatgcttctg aatattatct
     6481 gccccttcgt ggattcgttg agcaaaagcg ctacaaacag cccgagaagg agctggccgc
     6541 ttacacttcc cacgacccac acaattgtat ttagaagcgt tctgccaacc accggatctg
     6601 tgaagttatc tatgtagttt tgaagagtcc agtttccatt tttatcggta aaactgttga
     6661 aaaggttcga tccaagcaga aagacaaaga gtaaaacggt caagataacg taaggtaaaa
     6721 ccatcttgag agcggacttc tcttctctgt tcaatgtact cacttgtctt cagccctttc
     6781 ctcttcgaga attataaagg gggcgggcga ccactcccgc cccgaaagat agtgtatcac
     6841 tttgcaagaa ttgcgtttac atttgcttct gcagtcttta tagcttcttc cggcgttgca
     6901 ttaccaagga ggacattctg gatcatgatc tggatctgtt cagatacctg tggatactgt
     6961 ggaataggag gtctggaaac agcaaagggg aaagtttcga aaattacctg ccatttggaa
     7021 tcctgcagta cgggtgcggc atagcaaacg tcttttctgg tcggcatcgg accacccttg
     7081 gcctcgatcc atgtcatcca ggtctcgaag ctggtcaggt acttggcgag ctcccatgcg
     7141 ttttctttgt tcttgctcac cgatggaata acgagcgccc agccaccgag aacgctggcc
     7201 ggagtggaac cgtaaggatg gagtgagacg gtatagttgt tcactatcgc cggattggcg
     7261 gtctcgataa gagggaagga ccacggtccg ccgatagcca tggcaaccct gttctgtgcc
     7321 atcatgtttc tataatcgtc ttcgttgtaa gtaacggcgt tcgggctgac accataggtg
     7381 agagcgagat cggtgtagaa tttaagcgct ctcaggcctt cgggagtatt gaaggccgcc
     7441 gccgaataat cgtctgtcag tagctgtccg cctgcgccgt aaagaaagat gatgaatgcc
     7501 agagaggtgt gttctgtctt ggttccgctc acaccgtaac cgtaaactct cttgctggcg
     7561 tctgtgagtt tcgtggcctg gtaaaccagt tcctcccagg tttccggggg atcgtttcta
     7621 tccagtccgg ccgaatcgaa catagtctgg ttgtagagga ggagtctgca atcggtgtac
     7681 cacggaagac cgtacctttc accgttgtag ataactgtcg gccataggtt cggccatatc
     7741 tgttcgactc cgtcccagga atccaggaga gctcccatcg gttcgagcca gcctttctct
     7801 gcgaaggttg gaacccagcc gccaaggtcg gcaaaggcca gatccggtcc ctgtcctgct
     7861 tgaagggcgg tgaggagttt gtcgtagtaa atatcgaacc cgaacagctg cacctccacc
     7921 ttttgacctg tctccttctc gaatttaccg ataatcatat cccaggtttc atcgagttcc
     7981 gcgacctgtc cgcctatcca gatattgatc gtctgagata caccggcgat cgcaagcacg
     8041 acgagcataa gaagaaccaa taacgaagat ctgcgcatac taaaacctcc ctttttcggt
     8101 aagttacata tgtaacttac ttaagtaacc ataataattc tatacccttc tcttctctac
     8161 aaacaaatcc cgctgcacct gatcaggacg aattatcgat aatgacggtc gaagaaaggc
     8221 agatagctgc ccgattcttc atagatttta attcgctgac aagggggagt tccggtatct
     8281 ttccgaaaag ccgttttgcg taagacttct ggagatacgc caaaaagatt cgataatgaa
     8341 acggaaggag atcatgtgac tcattttttg tctttggagc ggcctttgtg ataatattct
     8401 tttgtcggct tgattggcat gaccgcatcc ctgaccgtct ttcataaaac ctgcggcctg
     8461 tttatctccc cgtagttatc gaataaaaag cgattcattc tggcttatat gagtattcac
     8521 gataatttgt aactgcttga tcgcctgaaa aaaggcgtca aaagagggga tgatagttgt
     8581 gaatcctggg aaagcgaagc tactgaaaaa tcttctcgtc ggtgcaaccg gtggtatcct
     8641 tacttttagc acagggttgg ctgcaaacta cgaagacgtt atgaccaaag aaacggttaa
     8701 acccgcgcca aacctggagg aagtaattcc acaccctgaa cagatgcaag aagccctcgc
     8761 caaactcgcc gagcttgaaa gcagaacggg aaaaaagccc aacattctga tctacctcat
     8821 ggatgacgtc gggtggggcg atctcggagt ttacggtgga ggtgtgatgg tcggagcacc
     8881 cacaccgaac atggatgccc ttgccagaga gggcctcatg ttgacttcgg cctattcaca
     8941 gccttcttct tcgccgacca gagccacctt gctgaccgga caactccccg tgagacatgg
     9001 aattctcaga ccgtcgttgt acaacgaacc gggaggattg ggagattcca taacactcgc
     9061 agaacttctg agcgaagctg gatacgttac acaggccgtc ggtaagtggc atctgggtga
     9121 aaatctagca tcacagcccc aaaacgtcgg tttcgatcat ttcaccggct ttctgagcgt
     9181 ctcggacatg tacacggagt ggcgcgatat cgatttctat cccgaaatcg ccctgagcga
     9241 agggcgcacc cagatgatgc gagattttgc cttcgagaaa tactgggtgc aggcatcgaa
     9301 gaacggcgaa ctcgagcgaa tagccgagat agatattgac gtgctcactt acctggatca
     9361 gaactgggcg gatttctcgg tcaacttcat aaaagagatg gccgacagcg accagccttt
     9421 cttcctgtac cacggtacca ggggcgcgca cttcgataat tatcctcccg aagagtacct
     9481 aggaaaatcg ccggctaaac agcgctacaa ggacacaatt gtagaactgg acgatatact
     9541 cggccgtctc gtcaaggctc tagaagaaac cggtcagctg gaaaatactc tgatattcat
     9601 cgcttccgac aacgggccgg aaatggaagc ctggccagac agcgcttact cacccttcag
     9661 aggggccaag ggaactacct gggagggtgg cgtaagggtt cctggaatac tgtactggaa
     9721 aggaatgata gagcccgggc gcgtctccga cggactgttc gatcttgctg acatattcaa
     9781 cacctctctg acgctggccg gggcgcaaga tctaatgccc accgacagat atatcgacgg
     9841 aatcgaccag acctccttcc tgctcgcgaa ggatgggctc tcgaacagaa agtacatcta
     9901 ctactggctg atgaactact tctcagccgt gaggatggcg gaatacaagt acatgacact
     9961 tgccgaagcc gacgatgaac ccgccggggt ttaccgagcc gggttcacag gcagtctcga
    10021 catgttcgcc tactaccaca tgtacaatct ttatctcgat cccaaagaag aacataacgt
    10081 ttccataaga aagctggtgt acaccgatct cttcctggcg gctatacaca aacacttcgc
    10141 tacactgaag cagtacccgc ctgcagtgga ggtattcctg gggctggttc gctgaaaaac
    10201 cggaaagaca ctttgcaaag cacggtaatc cacgaaattc tagatagaga aaagaataaa
    10261 aaggtacacc gggggtattc agccgaaggt gtacctttta aatgctcttc cgcccctatt
    10321 cctccagctc gaggctcaaa tacttggcct gcacgcctgg aagaacgttg agcttattca
    10381 tcagctcttc gtgtttctcc ctttcaccca ccagctcgag aatgatgagc ccataattgg
    10441 agcagctatc gagaacgccg tcgtgtatgc ccaggcgtgt cttgataaga cagccccatc
    10501 ctgtcagtac tttctgaacc tgtacggcct cttctttcct gttgtcaacc atgatagcca
    10561 ttatagtttt ctcgttgaat ttcatcacgc acccctcacg atttatatac tgacttgcct
    10621 gccagaatcg tctcaactac gttcagttca ctatccatta taacgagatc cgcgagatag
    10681 ccttccttta ttctccctct gtttctgact ccgaggttgg tcagagcgtt gtaagaagaa
    10741 actctggcca attcaaccag cgaacacccg gtccactttc ggaaattgcg gacggcctga
    10801 tcgagaacga gcgtactgcc ggctatcgtt ccgtcatcga gggtcgcttt cccttcattc
    10861 acggtcactc tcaacccgcc gaggtcgtag atgccgtctt ccagtcccgt cgcgtctata
    10921 gaatcggtaa cgaggattat attgtcggtc cccctgataa catagaccag ccttacgaac
    10981 tccggcgcgg tgtgaaatcc gtcgacaatc atctcgagct tgagcgggag cattaggccg
    11041 gcgccgacgc agcccacctc tctgtggtgg agtgtgttca tgccgttggg gaagtgcgtc
    11101 atcctgtcgc aaccggcctc gaaagcttta tttatctggg caaaggtggc atcggtgtga
    11161 cccatcgaaa cgactacgtc ctgcatcttc atctccgcca gcgcctcgaa gaaaccgtct
    11221 atctccggag ccatggttac cagaatcacg tcgtctgtga ggatctctct caactctttc
    11281 ggtgttgccg gtcttatagt ttcgggattc tgagcgccct tcttcttgac gtttatatac
    11341 ggtccctcgt agtgcactcc cctgatagac gttctggcct tctcttccat gtaggaggaa
    11401 acgagattag ccactctcaa catatctttc tttaaagccg agaccgtact cggaagaaga
    11461 aacgtgaccc cgtgaggata gagaaactcc tcccatttct cgaggccgat atgatccatc
    11521 ttcatactgt tgactccggc cgcgccgtgc gaatgggtgt caacgaaccc cggcatcatt
    11581 atcaacctgg gattgccgtg agttctgtca acggactcta tcttgttgcc agaaattctt
    11641 acattggcag tatattctcc gtcaaccgga tcgacgacaa gaacgttttt caactccatc
    11701 tggaaattcc ccctttcctg ctcaatcaat tatcacattc aaacggggaa tttaaaaccg
    11761 cagttgtcgc agaatctggc atcagaatcg ttctgctcgc cacaatcggg gcatttcttg
    11821 tcttttctca gagttgcgcc gcagtttttg cagaatctcg aatccggagc gttcccggtt
    11881 ccgcattttt gacatacgac ctttgactct tcggacccgg aaatcccttc ctttatagat
    11941 cttgcgatag tcttgacacc atcctttgtt ccttcggcca tatagttaat agtgtcttta
    12001 gccaccggcg caagctcgtt ggctttgtac cttgccacgg ctcccatgaa accgtaactg
    12061 gtcgcgatag ctcccagaaa gataaaaggg atcgccagga agaagaggta gaacttattc
    12121 ggctgcccga acgagccgaa agcgctgaag aaatctacca tggcaattat aaggatgacc
    12181 acacctataa aaataaggag tgggcctgca accctcaaaa tacttctcat gctactgttt
    12241 tttccgctaa cagacatatc ccgaactcct ttcatgaaat atcgaaaacg gtacagacaa
    12301 aacaccggcc tctagaggcc gtcctctgta ccagtatatc catatctttc cacaatgtct
    12361 ccaaaaaaag aagaccctct agaaagttat ggaaaaaggg tcttcgttta tctgataata
    12421 ctcttgtttg attttttcaa ttcacacgcc tcgccttcta atcagctgtg gggcgaggac
    12481 atgtatctct taatcgcatc gatcttcact ttctcgatca atatgtccac gtctgccaga
    12541 tttcttattc ttcttatcat cgcacacacc tccttttctg taatatcaga acctggggct
    12601 taccatcaga ctgctgtacg cactttctct taccttaaaa accattctgt cgatatcctt
    12661 caaacttctc tttctcatat tttctcctcc ttttatttgt gttctcttct cagaatctcg
    12721 gattgatcat caggttgctc aacgcgctgt ccttttcgct ctcgatcaac ttgtcaattg
    12781 ctctgaaact cttcttctct ctcttagaca tgacccttct cctcctttta gctctttctc
    12841 atctgatgtt catcattctt gcccttaaag aatcttcttt cgcgatttct acataacgat
    12901 ccatttcatg gtagtgcact ttcttctctc tcttgttcat cttcctcacc tccttttaca
    12961 gataatgtgg gaaggcttct ttctgtactt tgatcttgaa cttctggatc tctctctcga
    13021 tcgacgccgg gtctttaact tttctcattt cctcaccccc tttttgttat tttttccttc
    13081 gacattcata atatatcaca aagaataatc aaatgtcaag tggaaaaatc aaaaattcca
    13141 agattttttt caattattat tggaaatact gttcttttta tccagatcag tgcggtaaag
    13201 gttttctttt ttgcatatat caggctcata taggcgataa gcacaagtta tttctatatt
    13261 cacgaggatt tatatcataa ttttttatat ttatccaacc aaaattgcac aaaaatcaat
    13321 agaagaatat atgtatggag gacgaatcat ttacatatac catatatagg ggcctatccc
    13381 gtgtggtatt ggactacctg aagagttctc atataagtgg cttgaaaagc gcatattact
    13441 gatgggaaag aaatagtttt ggttgccttt gcatttcccg gaaagccggg aggagttcca
    13501 tattcaaagc gatcatcaaa tagtgggaat tatcgtattc ggacttcctt gagttgtgaa
    13561 tccaaaataa tcgaaatcga tctcattata attctt
//
LOCUS       DAOOLY010000034        13588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_4963_length_13588_cov_4.681815, whole genome shotgun sequence.
ACCESSION   DAOOLY010000034 DAOOLY010000000
VERSION     DAOOLY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13588
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_13588_cov_4.681815"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2321
                     /locus_tag="PLD15_02995"
     CDS             <1..2321
                     /locus_tag="PLD15_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB62896.1"
                     /translation="ANNQPPNGFTMLADIDTARDLLKINVPDYYNGLLVSNRGDFLGG
                     VKSTDAVAASMEKIAGREFKVETPKKDALDMADRGNIGLLFLMLSVFAIFAGTLLLSN
                     IYLMLAQERRMELGTLRAIGYTRRRVSRTILYEGFFYSILSSAIGVVTGVALANFIFE
                     SFVNLFTDITALIPFEGAATVFSTIQNSFGFFVRPESIFYGFLLGLIIPMIIIVYTGR
                     KISRTNIVTAVRNIPDEMDQKSRFMINIMASLGVLISIIMAYSGYSAGNATLFFVGVV
                     VTGLLFPVAIPLKNKRTIESLFSIGVILFTMFSNSFSLIAENSGDSVYLVVAKGGAIL
                     FAGLFLIVYNLKTFEYILNKIFQKTRSAAPVFKISIAFSARNRVRTGLTIAMYGVVIF
                     VITLISIIPYSQEQVLVKSRDMMFGGYDVGVFSLSTRNMVSFSDLQSRGEVSALTNIE
                     SINVAMKREERYVREYMYGLDDSFIVNNKFTEITFDKGTFKNAREVWEHLRSTPDTVV
                     VSSNVIPGILPGDTVELRRLVQREEDTGGMSSFMMRGMSDIIIGEERVKMTVIATLPE
                     NTITYMNGIFVYRGNIPESIDGKVSIRNYLLNLRGETQEEKKASFESLKDGMGSGSLF
                     MIFVDDIIELTTTIIQGTISILRSFLYFGMLVGIVGIAIIMFKALYERKRIVGMLKAI
                     GFTKKMVFASFVLETSFIVVIGIVLGIITGTLTSVEIYASPLMQGMALSIPWDQLLGM
                     SLIFYVASLVSTIIPSYIASKIPPAEAFRYFE"
     gene            2337..3455
                     /locus_tag="PLD15_03000"
     CDS             2337..3455
                     /locus_tag="PLD15_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HPB62897.1"
                     /translation="MSRILVVDDDRAVRVLLKSILVKDSHEVIESPDGSSALLAAREE
                     RPDLILLDLMLPDYNGLEVLSGLKEDSDLEVIPVIVLTGSSDRQSKLTALSSGAVDFI
                     SKPFMSEEVLLRVNTQLKLHNLIKSLRVAVDSLQNDVLAAGRIQAALVPQSAPQGLKM
                     EWIYSPSYRVGGDIFDVVKLGEEKFFVYLADMSGHGVNAAMLSVMVHRFIEDYRGRIG
                     EGEFDLKDFMAELDSSFRFEKFNLFFTIISMIIDTASKKLLLCNAGHPSPLLMENGRF
                     RVLENKEGLIGMNMIRGAIEELNLSGGDRLILYTDGFTESTNEDGEMFGEERLQDILQ
                     GCLDMSLSATKENLRDRFEKFKGIKNAEDDVSLLLIEF"
     gene            3530..3871
                     /locus_tag="PLD15_03005"
     CDS             3530..3871
                     /locus_tag="PLD15_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPB62898.1"
                     /translation="MFKFELLKKSIGVISLEKPVRITGETSASFRKWYESKDLASCSA
                     VIIDGNNIQFIDSMGIAALISIYKSLTPKGSNLHLVNLSPEIKKLLATLRLDRLFQIH
                     DGTVEEAVKSL"
     gene            3882..4283
                     /locus_tag="PLD15_03010"
     CDS             3882..4283
                     /locus_tag="PLD15_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB62899.1"
                     /translation="MRQSKQRFSVRPLMGEVDSVSARITEIVKEVAGEERGFRAGLVV
                     TEACVNIVKYGDLGEEERIEIELSFEDNDAKIVIEDTSREFNPLDVSDPNLNNFELLQ
                     KGGMGIYLIKRCSKKVDYAYKDNKNILTVII"
     gene            4534..6273
                     /locus_tag="PLD15_03015"
     CDS             4534..6273
                     /locus_tag="PLD15_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPB62900.1"
                     /translation="MKDLSVSFDSLDVLKKIDLDLLPGEVLALTGPNGCGKTTLLRAI
                     TGEIAPSGGSVQIERGYWPVYVRQEDVDYRGSTGNYLLGARPELLKLHEKLEAAADPL
                     AYAEYINEYHATGGFDFEEEIALTLVDFGFESDEIHKGFNEHSQGQKRLLSIMRALLS
                     RSKLLLLDEPTNHLDIAMTLRLEGIVNRLKRRGTGTIVVSHDRTFIDRVADRTIYLKF
                     GQSLSVNGGYSLMLEHLESDFRSREKEAGEIDRKIRQLEAEVVRRKNWSASKEASKRY
                     AMDKGYVGHMAAKQARRAMAVQKRTGKLIDELKERKPFVEKPLSFSLSDYTVSRRKMV
                     SAEDISFSYETDEIISGAFMDIDTTDRVGILGQNGSGKTTFMKCLIGELEPSSGKIYR
                     NEGVRWAYIPQNVLDFFEREMLIEEFLSTGIEEEKLRSHLANIRFRKERAMARISDLS
                     HGELMRAALLKVIVSKAEFIFMDEPTNHLDIESLEVMDDLLGDFPGGFVFISHDRRFI
                     ASHGQRLFFIDRGVLKSFESMSRVDVRLFRQISDDISDAAREAEMRRRRSQNDWESVE
                     KAPLEGKNEDTGN"
     gene            complement(6288..6848)
                     /locus_tag="PLD15_03020"
     CDS             complement(6288..6848)
                     /locus_tag="PLD15_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HPB62901.1"
                     /translation="MKESEYIRLLEKGLGSLSAVERQELLQDYRDHFREGKKAGKSEE
                     EISRALGDPGKIARAVAAEYRVSSIEERSGKGRGIGAAIVSIIGLSFFNLVVSLPFFI
                     VLYALVLSLYAVAAAFVASPFILFFFPYLVNLPLDITQLQLLLFSFGLFFIGLAIFFM
                     SGKIWNGVLELTVRFLKWNINVFKGS"
     gene            complement(6835..7152)
                     /locus_tag="PLD15_03025"
     CDS             complement(6835..7152)
                     /locus_tag="PLD15_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPB62902.1"
                     /translation="MKKGVLDLCVLSILDREDCYGYQLVERVSSAVEISEGTIYPLLK
                     RLQKENLLSSYIIESSEGPARKYYRLTDSGRSRFMDMKRDWREFAERVNALIGGEVDN
                     EGI"
     gene            complement(7208..8275)
                     /locus_tag="PLD15_03030"
     CDS             complement(7208..8275)
                     /locus_tag="PLD15_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPB62903.1"
                     /translation="MKGFERKRGVAWVGILIAIGLILIGVAVAIGPAGIDFGFDFGWS
                     LDGQRFDRSYQESLNDAIKSIDIYTLNGEVEVVGWEESFISLEVKQTVKVSSENLKEK
                     YFEETKPVVTILDGLLRIVTPRLQQTGEFRGQGASIYLRVPIELLEEVVARTSNGRLK
                     AASLNSYVNVESSNGGIEIYSINGRVVAETSNGPINARDIVGPMEFQSSNGPFTGARL
                     NGDLRVTTSNASINVETSSSNISALTSNGSINITDCILQGTKNSFRTSNGKVTVNSTL
                     SSQGTLELASSNGQILLFVPEDIRAYIDASTSNGRVDMTGLSITVSSMSKTSVRGNLN
                     NGSDLDITLRTSNADIVIKKK"
     gene            8629..9132
                     /locus_tag="PLD15_03035"
     CDS             8629..9132
                     /locus_tag="PLD15_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB62904.1"
                     /translation="MKKGFSLVELLMVVVIIASLISVATIVSASILRKTKATTVARDM
                     KAIMMAAEEKLMIEGPKYFQSIKSLKDLVENATDKYQLYLVIKKSGDVYITVICDPSH
                     VDVELVREISPDATVLSTGAKCYLNDEEVFHKITYNSNMVHYLVGSYVTYIPENRICI
                     FRDYRLF"
     gene            9242..10303
                     /locus_tag="PLD15_03040"
     CDS             9242..10303
                     /locus_tag="PLD15_03040"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HPB62905.1"
                     /translation="MKIKSHEPALPLIQGGMAVGISLDGLSAAVASAGGVGTIGTAGI
                     GMTIEGYARNFKQASIEGLKNVIRKARSKTDGLLGVNIMVALTNYQDMVVTALREKID
                     MIISGAGLPLDLPSYLEEGSKTAIIPIVSSLKSAKVILKRWFGKFRYIPDAFIVEGPK
                     AGGHLGYRVEDLQSQEFSLSSTIPQVRQLTEEIKREYGKDVPVIAAGGIFEREDVQEM
                     FELGASGVQVGTAFIGTQECDADIRFKQALIEAKKEDIVIIKSPVGMPGRAIRNKFLV
                     DVEAGNKKPFKCVYHCIKTCNFRETPYCIAQALMNACKGDLENGFAFSGTEGYRIKEI
                     TTVKDVIERLFGTGPLSLY"
     gene            complement(10260..11183)
                     /locus_tag="PLD15_03045"
     CDS             complement(10260..11183)
                     /locus_tag="PLD15_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB62906.1"
                     /translation="MAGNSKRVYSEKSKSILLMLIASALWSTGGLFIKNVNAHPLAIA
                     GVRSAIAAVVIFAANRKPKFNWSLPQIGAALSYVGLVFLFVSATKLTTSANAILLQFT
                     SPIYVAILGAWILKERVKLFDWITISIVFGGMVLFFLDKLDPRGVLGNLLAIGSGVCF
                     ALLTVFMRMQKDGSPMESILLGNLLTAVIGIPFIAFGVPDAFDWLILLVMGILQLGIP
                     YVLYSRAIKHVTALESVLISGIEPILNPFWVFLLIGEVPGPLALVGGCIVFVIVTVRC
                     IISAFPPSGSRKAIMVTISKDSTDPSRKDVR"
     gene            complement(11318..11947)
                     /locus_tag="PLD15_03050"
     CDS             complement(11318..11947)
                     /locus_tag="PLD15_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HPB62907.1"
                     /translation="MADARETNAVKALQATANPSPLGLFGLAIVTLVASTQKLGITEG
                     TVNIIPWAIFLGAAAQLLAGFMDYRLNNTFGATAFSGYGFFWLGVAMTWMMGNGIIAM
                     NPAAVVDTSQLAFAFLGYLIFTLYMTLGAMGTSKVLFVIFFLIDILFLGLFVSILGVG
                     GVFWKYLAGCAEMGIAIFSFYGSAANLLNTHYGKTVLPAGKAFGPWAMK"
     gene            complement(12159..13538)
                     /gene="gltA"
                     /locus_tag="PLD15_03055"
     CDS             complement(12159..13538)
                     /gene="gltA"
                     /locus_tag="PLD15_03055"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPB62908.1"
                     /translation="MPEKNKTKMRELDPIERIGCFEEVALGYSREEALKEAARCLQCK
                     NQPCVDGCPVGINIPGFIKALREERYKDSTVILKGDNNLPAVCGRVCPQEKQCEARCI
                     LGRVPGGDAVAIGRLERYVADLEIPIESVVKPPVHKKVAVIGAGPAGLTVAADLAKEG
                     VSVTVFEAFHTAGGVLVYGIPEFRLPKEIVSNEVQFVKEMGVEFKFNEIVGRTVPFEE
                     LLSDYDAVFIGIGAGAPRFMGIPGTNLNNIFSASEFLTRVNLMKAYLFPKYDTPVKIK
                     DRVAVIGAGNVTMDAARTAKRLGAKDVHIVYRRSEEEMPARLEEYHHAMEEGIKLHCL
                     TLPVEYLGDSNNNVIAMKCVKMILGEPDSSGRRRPVQLENSEFTMEVDMVIEAIGQTP
                     NAVLKAGFPSVQLNKWGSIQADEETGRTNFPNVFAGGDIVTGAATVIEAMGAGKRSAK
                     AILEYLAGA"
BASE COUNT         3587 a         3328 c         3412 g         3261 t
ORIGIN      
        1 aagcgaacaa ccaacctcca aacggtttca cgatgctcgc ggatatagac accgcgagag
       61 acttactcaa gataaacgtt cccgattatt acaacggcct tctggtatcc aacagggggg
      121 attttcttgg cggagtcaaa tcgaccgatg cggtagcggc gtctatggag aagattgccg
      181 gcagagaatt caaggtcgag acacccaaaa aggacgccct cgatatggcc gatcggggca
      241 atatcggact gttatttctc atgttgagcg tttttgcgat ttttgcgggg acactgcttc
      301 tctcaaacat atatctcatg cttgcccagg aacgaaggat ggagctcggt acgctgagag
      361 cgataggtta tacaagacgt agagtctcca ggaccattct ttacgagggc ttcttctact
      421 ctatactctc ctcggccata ggcgtggtaa cgggtgtagc gctcgcgaac ttcatattcg
      481 agagcttcgt caatcttttc acggacataa ccgctctgat acccttcgaa ggggcggcca
      541 cggtgttcag caccatacag aactccttcg ggtttttcgt acggccggaa tcgatcttct
      601 acggcttctt gttgggcctg ataataccca tgataataat cgtctatacg ggaaggaaga
      661 tatccagaac caacatagtt acggcagtca gaaacatacc cgatgagatg gatcagaaga
      721 gcaggttcat gatcaacatt atggcctcgc tgggtgtgtt gatctcgatc atcatggctt
      781 acagcggtta ctcggccgga aacgccacgc tcttcttcgt tggagtcgtc gttaccgggc
      841 tgcttttccc ggtggcgata ccactcaaga ataaaagaac gatcgaatcg ctcttttcca
      901 taggagtgat actcttcacg atgttcagca attccttctc cctgatagcc gaaaacagcg
      961 gagattccgt ctatctggtc gtggcgaagg gcggcgcgat actcttcgcc ggtctcttcc
     1021 tgatagttta taacctcaag accttcgaat acatactcaa caagatcttc cagaagacga
     1081 gatcggcggc gccggttttc aagatatcga tcgccttctc cgccaggaac cgtgtcagaa
     1141 ccgggttgac gatagccatg tacggtgtcg tcatctttgt aataacgttg atttcaatca
     1201 tcccttactc ccaggaacag gtgctcgtca aaagcagaga catgatgttc ggggggtacg
     1261 atgttggtgt cttctcactc tctacaagaa acatggtctc cttcagcgat ctgcaatcta
     1321 gaggcgaagt ctcggccctc accaacatag agagtataaa cgtggcgatg aagagagaag
     1381 aaagatacgt ccgtgaatac atgtacggac tggacgacag tttcatagtc aacaacaagt
     1441 tcacggaaat aacgttcgat aaaggcacgt tcaagaacgc cagagaagtc tgggagcatt
     1501 tacggtctac ccccgatact gttgtagtct cttccaacgt cattcccgga atactccccg
     1561 gagataccgt cgagctgaga agattggttc aacgagagga agataccggc ggtatgtcgt
     1621 ctttcatgat gagaggcatg agtgacatca taatcggcga ggaacgcgtt aaaatgacag
     1681 tcatagccac actcccggaa aatacgataa cgtatatgaa cgggatattt gtgtacagag
     1741 gaaacatccc cgaatccatc gacggtaagg tctcgatcag gaattacctc ttgaatttga
     1801 ggggagaaac tcaagaagag aaaaaggcga gtttcgagtc tctgaaagat ggtatgggtt
     1861 cgggaagtct ctttatgatc ttcgttgacg atataataga gttgacaacg acgattatac
     1921 agggaacaat aagtattctg agatccttcc tgtacttcgg tatgctcgtc ggaatagtcg
     1981 gtatagccat aataatgttc aaggcccttt acgaacgcaa gaggatagtc ggcatgctca
     2041 aagccatagg attcacaaag aagatggtct tcgcctcttt cgttctggag acatcgttca
     2101 tagtcgtaat cgggatcgtc cttggaataa taaccggtac cctcaccagc gtggaaatct
     2161 atgcctcccc tctcatgcag ggcatggctc tttccattcc ctgggatcag ttgctgggaa
     2221 tgtcactgat attctacgtc gcatcgctgg tttcaacgat aataccgagc tacatcgcat
     2281 cgaagatacc tccggcagag gcgtttaggt atttcgaata aaaaggaggt tctcgaatga
     2341 gcaggatact cgtcgtcgat gacgaccggg cggttcgagt tctcttgaaa tccatactcg
     2401 tcaaggacag tcatgaagtt atcgagagtc ctgacgggag ttcggcgttg ctggcggcca
     2461 gagaagagcg gcccgacttg atcttgctcg atctcatgct ccccgattat aacggactgg
     2521 aagttctatc gggactcaaa gaagacagcg atctggaagt tataccggtg atagttctaa
     2581 cgggttcgtc agaccgccag agcaagttaa cggccctgag ttctggagcg gtagatttca
     2641 tatccaaacc cttcatgtcg gaggaggttc tgctaagggt caatacacag ctcaaattgc
     2701 acaatctcat aaaatctttg agggtagcgg tggacagcct gcagaacgac gttctggccg
     2761 caggtagaat tcaggcggcc ctcgtacctc agagcgcacc tcagggactg aagatggaat
     2821 ggatctactc tccctcgtac agagtgggcg gcgatatatt cgacgtcgta aaactgggag
     2881 aggaaaagtt ctttgtctat ctggccgata tgtcgggtca cggtgtgaac gcggcgatgc
     2941 tttcggtcat ggttcacaga ttcatcgagg attacagggg aaggattgga gagggggaat
     3001 tcgaccttaa ggacttcatg gccgaactgg acagcagttt caggttcgag aagttcaatc
     3061 tcttcttcac gatcatctcg atgatcatag ataccgcgag taaaaaactc ctgttgtgca
     3121 acgcggggca cccctctccg ctgctgatgg aaaatggaag gttcagggtt ctcgaaaaca
     3181 aggaaggcct gataggcatg aacatgatac gcggagcgat cgaagagctc aacctttcgg
     3241 gaggggacag gttaattctc tacacggatg gcttcacaga gtccactaac gaagacggag
     3301 agatgttcgg cgaggaaagg ttgcaagata tcctgcaggg ctgcctggat atgagccttt
     3361 ccgcaaccaa agagaatctg agagatcgtt tcgaaaaatt caagggaatc aagaatgccg
     3421 aagacgatgt ctcgctattg ctgatagagt tttagcgtag aggatctcca cgtcgaggaa
     3481 ggtaagttga aaaaaggagg gattccacaa cgttggttgt gggtgcatta tgtttaaatt
     3541 cgaattgctc aaaaaaagta ttggtgtaat ttccctggag aaacccgtta gaattaccgg
     3601 ggagacatcg gccagtttcc gcaagtggta cgagagcaag gatctggcca gttgttccgc
     3661 cgtgataatc gacggcaaca atattcaatt catagactcg atgggaattg ccgcgttgat
     3721 cagcatatac aagtctctga cgccgaaggg aagcaacctc caccttgtga acctgtcccc
     3781 ggagatcaaa aaactcctgg ccacgctccg actggacagg ctcttccaga ttcacgacgg
     3841 cacagtcgaa gaggctgtaa agtctctgta agataggttg gatgaggcag tcgaagcagc
     3901 ggttctcagt tagacctctg atgggggagg tagattcggt ctcggccaga ataacggaaa
     3961 tagtcaaaga ggtagccggc gaagagcgtg gtttcagggc cggcctggta gtcaccgagg
     4021 cctgtgtgaa tatagtcaag tacggtgatc tgggagagga agagaggatc gagatagagc
     4081 tcagcttcga agacaacgat gcgaagatag ttatcgaaga cacttcgcga gagttcaacc
     4141 ccctagatgt gagcgatcca aacctcaaca acttcgagct cctacagaag ggcgggatgg
     4201 gaatctacct catcaagaga tgctccaaaa aggttgacta cgcatataag gacaacaaaa
     4261 acatactgac ggtcattatt tgatttcgaa aatcgcggct ggaccacttg aaccggcacg
     4321 cagatatatg ttataaagta cacaggtcaa aactgtaaag gtgcagaaat gaagaaacta
     4381 ccccggtaat cggcacaatt gaatatcgct ttcccgtaat cgactcgtgt taagcattcg
     4441 cgtcattttt ttcaatggct caacacagcc aacacttaca tttaataacg aaaattctca
     4501 caggccataa tggaggtggt ttctttgcta cacgtgaaag atctatccgt atcgttcgac
     4561 agtctcgatg ttctgaaaaa aatagatctc gacctgttac ccggggaggt actggcgctc
     4621 accggcccca acggctgcgg caaaacgact cttctcagag cgataaccgg cgagatagcc
     4681 ccttccggcg ggagtgtgca gatcgagcgt ggttactggc ccgtttacgt ccggcaggaa
     4741 gatgtcgatt accgcggaag cacgggcaac tacctgctcg gcgcgagacc ggagctgttg
     4801 aaattgcatg aaaagctcga ggcggccgcc gacccactcg cgtacgccga atacataaac
     4861 gagtaccacg cgaccggggg cttcgacttc gaagaggaga tagcactgac gctcgtggat
     4921 ttcggcttcg agagcgacga gatccataaa gggttcaacg aacattcaca gggtcagaag
     4981 aggcttctct ctataatgag agcgctcctc agccggtcga aactcctcct gctggacgag
     5041 ccgaccaacc atctcgacat agccatgacg ctgaggctgg aaggaatcgt caacagactc
     5101 aaaagacggg gaacgggaac aatcgtggtc agccacgatc gtactttcat agacagggta
     5161 gccgacagga cgatctatct gaagttcggt caatcgttat ccgtcaacgg cggttactcg
     5221 ctgatgctcg aacatctgga gagcgatttc cgctccagag agaaagaggc gggagagatc
     5281 gacaggaaga ttcgtcaact ggaggccgag gttgtgagaa ggaagaactg gtcggcctcc
     5341 aaagaggctt ccaaaaggta cgcgatggat aaaggttacg ttggccacat ggccgccaaa
     5401 caggccagac gggcgatggc cgtccagaaa agaaccggaa aattgataga cgaactgaag
     5461 gaaagaaagc cctttgtcga gaagccactc tctttctctc tttcagatta caccgtttcc
     5521 aggagaaaga tggtgagcgc cgaggatatt agcttctcct acgaaacgga cgagatcata
     5581 agcggggcct ttatggatat agacaccacc gacagggtgg ggatactcgg gcagaacggt
     5641 tccggcaaga cgacgtttat gaagtgcctg atcggagagc tcgaaccttc atcggggaag
     5701 atttacagga acgaaggcgt tagatgggcc tatattcccc agaacgttct cgatttcttc
     5761 gagagggaga tgctgatcga agagttcctc tcaacaggaa tagaggagga gaagctgaga
     5821 agtcacctcg cgaacatccg cttcaggaag gagcgggcga tggccagaat atccgatctg
     5881 agccatggag agttgatgag ggccgccttg ctcaaggtga tcgtttcgaa ggccgaattc
     5941 atattcatgg acgaacccac caaccatcta gatatagaat cgctcgaagt catggacgac
     6001 ttgttggggg atttccctgg cggattcgtc ttcataagcc acgacagacg cttcatagcc
     6061 agtcatggcc agaggctttt tttcatcgat cggggcgttc tcaagagttt cgaaagtatg
     6121 tccagagtcg atgttcgcct tttccgacaa ataagcgacg atatatccga tgccgcgcgc
     6181 gaggcagaga tgaggcgcag acgatcgcag aacgattggg agagtgtcga aaaagcccca
     6241 ctcgaaggaa aaaatgaaga tacgggaaac tagaagggga ctctcttcta gcttcccttg
     6301 aaaacattta tgttccattt caagaaccta accgttagct ccagaactcc gttccagatc
     6361 tttccggaca taaagaagat ggccagcccg atgaagaaaa ggccgaagct gaagagaagc
     6421 agttgtagct gtgttatgtc gagcggtaga tttacgaggt acggaaaaaa gaacagtatg
     6481 aacggactgg caacgaaggc cgcagcgaca gcgtaaagtg agagaacaag cgcgtacagg
     6541 acgatgaaaa agggcaggga aaccaccagg ttgaagaaac tcagcccgat tatgctcacg
     6601 atcgcagcgc ctatgccgcg cccttttccc gatctctctt cgatactgct gactctgtac
     6661 tccgcggcta cggcgcgggc aattttcccg gggtcgccga gcgcgcgcga tatctcctct
     6721 tcgcttttgc cggccttttt accctctctg aaatggtcgc gataatcctg aaggagctcc
     6781 tgcctctcaa cagcggagag ggagccaagc cccttttcga gcagccttat gtattcagat
     6841 tccttcattg tcaacctccc cgccgatgag agcgttcacc ctttcggcaa actctctcca
     6901 atctcttttc atatccatga atctcgatct tcccgaatcg gtcaacctgt agtattttct
     6961 tgccggtcct tcgctggact ctattatata cgaagagagc aggttttctt tctggagtct
     7021 cttcaaaaga gggtatatgg tgccttcgga gatctcgacg gcgctagaca ccctctcaac
     7081 cagctgataa ccgtagcaat cctccctgtc gagaatactg agaacacaaa gatctagaac
     7141 acccttcttc atctggctat ccaagtaaaa caccccgtaa cggagacgat cgcccggttc
     7201 tggtcattta tttcttcttt ataactatgt cggcgttgct ggtcctgaga gttatatcca
     7261 gatcgctgcc gttgttcagg tttcccctta cgctggtctt gctcatgctc gatacggtga
     7321 tggagagccc ggtcatatct accctgccgt tggaggtcga ggcatcgatg taggcccgga
     7381 tgtcttcggg aacgaatagc aatatctgac cgttcgaaga ggccagctca agcgtcccct
     7441 gcgacgagag cgtagaattc accgttacct tgccgttgga ggtcctgaag ctattcttcg
     7501 ttccctgcag aatgcagtct gtgatgttga tggatccgtt ggaggtcagt gcgctaatat
     7561 tcgatgagga agtttccacg tttatcgacg cgttcgaagt ggtaacccgc aggtcgccgt
     7621 tcaacctcgc gccggtgaat ggaccgttgc tgctctggaa ctccatagga cctacgatat
     7681 ctctggcgtt gatcggtccg ttcgaagtct ccgctactac tctcccattt atcgagtaaa
     7741 tctctattcc gccgttagaa ctttccacgt tgacataaga gttcagcgag gccgctttca
     7801 gcctgccgtt gctcgttctg gccactacct cttccagcag ttcgatggga acgcggagat
     7861 atatcgaagc gccctgtccc ctgaattcac cggtctgctg gagcctcggc gtaacgatcc
     7921 ttaacagtcc atccagaatg gtcaccacgg gcttcgtttc ctcgaagtat ttctccttga
     7981 ggttttccga ggaaaccttc acggtttgtt tgacttcaag agatatgaaa ctctcttccc
     8041 aaccgacaac ctccacctcg ccgttgaggg tgtagatatc tatcgatttg atcgcatcgt
     8101 ttagcgattc ctgatagctc ctgtcgaaac gttgaccgtc gagagaccag ccgaagtcga
     8161 aaccgaaatc tataccggcc ggtccgatcg ccaccgccac tcctatgagt atcagaccga
     8221 tagctatcag aatcccaacc caggctaccc cgcgctttct ttcaaaacct ttcaattcga
     8281 ttacctccca ttcgtccggt gaagaggccg gacttcgatt cagcgaataa tatatcacct
     8341 attacctggt attgcaaggt actgtgcaaa tttgttgaat taagattttg ttaatattta
     8401 agagcaggaa aagccgccct tcgttgttta ttagaataag agaggcaggg gatgcacatc
     8461 cagataaggc aagtatggtt ataattcgct tctttaactc gaaacgcctg agttgatagg
     8521 gccaaagagt tttgagcgtg tcaagaacag aaagcagtgt ttctatatta ttctttcaat
     8581 atttctgtat atattatttg agtatatata atttgacgga ggtttagcat gaagaagggt
     8641 ttttcacttg tcgagctttt gatggtagtc gtgataattg cctcgttgat ttcggtcgcg
     8701 acgatcgtgt ccgccagcat tctcagaaag acgaaagcga cgacagtagc acgagacatg
     8761 aaagccataa tgatggcagc tgaggaaaag ctaatgatcg aagggccaaa atacttccaa
     8821 agtatcaaaa gcctgaaaga tctcgtcgaa aatgcgaccg ataagtacca attgtatctg
     8881 gtgataaaga aaagcggaga tgtttatatt acagtgatat gtgatccttc acatgtggat
     8941 gtagagctgg tgcgcgaaat atcaccagat gcaactgttt taagtaccgg agcaaaatgc
     9001 tatctcaatg atgaagaagt tttccacaag atcacttaca atagcaatat ggtacattac
     9061 ctcgtaggtt cctacgtcac atatatcccc gaaaacagaa tttgtatctt tagagattac
     9121 cgccttttct gagcaatcga aacgggaaac tgacatcgcg ctaacccctc agggatgttt
     9181 tacagttaga atttataatc caatcttatc tggaccggaa ggggtgagag atataggcat
     9241 cttgaaaata aagagtcacg aaccggcact accgctgata caggggggaa tggccgtagg
     9301 catttctctt gacggtctct ctgcggcggt cgcttccgcc ggaggcgtgg gtactatcgg
     9361 taccgcgggg atcggtatga cgatagaagg gtacgctaga aacttcaaac aggccagtat
     9421 agaaggattg aaaaatgtga taaggaaagc tcgtagtaaa accgacgggc ttctcggcgt
     9481 gaatataatg gtcgccctga ccaattacca ggatatggtc gtaacggctt taagggagaa
     9541 gatagacatg ataatctccg gtgccggttt gcctctggac ctgccttctt atctggaaga
     9601 aggttcgaaa acggctataa taccgatagt ctcttctctt aaatcggcga aggtgatcct
     9661 gaagagatgg ttcggaaaat tccgctacat tccagacgct tttatcgtag aagggccgaa
     9721 ggctggcgga catttaggct acagggttga agacctgcaa tcacaggagt tttcgctttc
     9781 cagtaccatc ccgcaggtac gtcaactgac agaagagata aagcgcgagt acggaaagga
     9841 tgtacccgtc atagccgcgg gtggcatatt cgaacgcgaa gacgttcagg agatgttcga
     9901 acttggagcc tcgggcgtac aggtcggaac ggccttcatc gggacacagg agtgcgatgc
     9961 cgatattaga ttcaaacagg ctctgataga ggcgaaaaaa gaggatatcg ttataatcaa
    10021 aagtcccgtc ggtatgcccg ggagagcgat caggaataaa ttcttagtcg atgtggaggc
    10081 cggaaacaag aagcccttca aatgtgtcta tcattgcata aagacctgca actttagaga
    10141 gaccccctac tgtatcgcgc aagctctaat gaacgcgtgc aagggcgacc tggaaaacgg
    10201 tttcgccttt tcaggaaccg agggctacag aataaaggaa ataaccaccg taaaagatgt
    10261 catcgaacgt cttttcggga cgggtccgtt gagtctttac tgatagtcac catgatggcc
    10321 tttctcgaac ctgacggagg aaaggcggag attatacagc gcaccgtgac tatcacgaag
    10381 actatacagc cgcccaccag agccagcgga cccgggacct cgccgatgag aagaaagacc
    10441 cagaacgggt tcaatatcgg ctctatgccc gagattagaa ccgattcaag cgccgttacg
    10501 tgttttatgg ctctcgaata gagtacgtaa ggaataccca gctgcaaaat ccccataact
    10561 aaaagaatta gccagtcgaa ggcatccggt accccgaaag cgatgaaggg tatccctata
    10621 acggccgtca acagattgcc cagtagaatc gactccatag gcgagccatc tttctgcatt
    10681 ctcataaaga ccgttaaaag ggcgaagcaa acgccactgc ctatggcgag taagttgcct
    10741 agcactcctc ttggatcgag tttgtcgagg aagaagagga ccatacctcc gaagacgatc
    10801 gaaatggtta tccagtcgaa gagcttcacc ctctctttga gaatccaggc acccagtatc
    10861 gcaacataga tcggagatgt gaactgaaga aggattgcgt tggccgaggt agttagcttg
    10921 gtcgcgctca cgaagagaaa gaccagaccg acatacgaaa gcgccgcccc gatctgggga
    10981 agcgaccagt tgaatttggg ctttctgttc gcggcaaaga ttaccactgc agctatggcg
    11041 cttctcacac cggctatagc aagagggtgc gcgttaacgt tctttatgaa taaccctccg
    11101 gtactccaga gcgctgaggc gatcaacata agaagtatcg atttcgactt ttccgaatat
    11161 actctcttcg aatttccggc cattctatta actcccaccg cccgggtgcc gctcttacag
    11221 taccctgccc ggaaaattat agcaccgtat ccgattcggt gcgaaccggt gaacggtgca
    11281 aagacacata ccacctgtga agtttcaatc tatctttcta tttcatcgcc cacggaccaa
    11341 acgctttgcc ggcaggaagc accgtctttc cgtaatgagt gttcagcaag ttagccgccg
    11401 aaccatagaa tgaaaagatc gctatgccca tctctgcaca accggcgaga tatttccaga
    11461 aaactccgcc gacacccaga atactaacaa ataagcccag gaagaggatg tcgattagaa
    11521 agaagattac gaagagtacc ttactggtac ccatagcccc caatgtcata tagagggtga
    11581 agatcaaata tccaaggaag gcaaaggcaa gttgagacgt gtccacaacc gctgctggat
    11641 tcatggcgat aatgccgttt cccatcatcc aggtcatggc aactccaagc cagaagaaac
    11701 cgtacccgga gaatgcggtg gctccgaaag tgttgttcaa cctataatcc atgaagcctg
    11761 ccagaagctg ggccgccgcg ccaagaaaaa tcgcccatgg aattatgttc acagttcctt
    11821 ccgttattcc gagcttttga gtcgaagcta ccagagtcac gatagccagt ccaaacaacc
    11881 cgagtggcga aggatttgcc gtcgcctgga gcgcctttac cgcgtttgtt tctcttgcat
    11941 ctgccatata tgcctcccga catcactgga gtgccggagc actccagtct gtaagtctgt
    12001 atttacaaaa gttagggatg tgtaatcaaa aaaatggtta cgaagaaagt atctccttcg
    12061 agaggaaacg ataaatcgta ccgctaatat ttttctatcg tatgaacgac tacttcacat
    12121 atgtatggaa gtactcagct tctaatgata ctcctggctc aggcgccggc caggtactcc
    12181 agtatagcct tggcggatct ctttcccgca cccatggctt cgataacggt cgcggctccg
    12241 gttactatat ctcccccggc gaagacgttc gggaagttgg ttctgccggt ttcctcgtct
    12301 gcctgtatgg atccccattt gttgagctgg acagagggga agcctgcttt gaggaccgcg
    12361 ttgggtgtct gaccgatcgc ctcgattacc atatctactt ccatggtgaa ctcggaattt
    12421 tccagctgca cgggacgcct tctgccggac gaatcgggtt ctcccagaat cattttcacg
    12481 cacttcatgg ctataacgtt gttgttcgag tctcccaaat actccacagg cagagtcaga
    12541 cagtggagct tgatcccctc ttccatggcg tgatggtact cttcaagcct cgcgggcatc
    12601 tcttcctcgc tccgcctgta aacaatatgg acatccttgg caccgagtct tttcgccgtc
    12661 cttgcggcat ccatcgtaac gttaccggcg ccgatcaccg ctacacgatc ctttatctta
    12721 accggagtat cgtacttcgg aaagaggtag gctttcatca gattgactct tgtgaggaac
    12781 tccgaggccg aaaatatatt gttcaaattc gttccgggta ttcccatgaa tctaggcgca
    12841 ccggccccga ttcctatgaa aaccgcatcg taatcactca agagttcttc gaagggaacg
    12901 gttcgtccca caatttcgtt gaatttgaac tcaacaccca tttcctttac aaattgcacc
    12961 tcgttcgaaa ctatctcttt tggaagcctg aactcgggaa ttccgtacac aagaacgcca
    13021 ccggcggtgt gaaaggcttc gaacacggtt actgaaactc cttcttttgc cagatcggcc
    13081 gcaacggtca atccggccgg accggcgcct atgactgcga cttttttgtg aaccggtggt
    13141 ttgacaacgc tttcaatagg tatctccagg tcggcgacat atctctccag tcttccgatg
    13201 gcaacagcat cgcctccggg tacccttccg agaatacacc ttgcctcaca ctgtttctcc
    13261 tgaggacaga cccttccgca gaccgccgga aggttgttgt cacccttgag tatcacggtg
    13321 ctatctttat acctttcttc tctgagtgcc tttatgaaac ccggaatgtt tattccaacc
    13381 ggacagccat caacacaagg ttgattcttg cactgaaggc atctggcggc ttccttcaaa
    13441 gcctcttctc tactgtatcc cagtgccact tcttcgaagc atccgatcct ttcgatagga
    13501 tcgagctctc tcatcttagt cttatttttt tcaggcattc ttaccaccca tttctttcac
    13561 ataatcttcc atggcctgtt tctcttct
//
LOCUS       DAOOLY010000035        13485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5016_length_13485_cov_4.987565, whole genome shotgun sequence.
ACCESSION   DAOOLY010000035 DAOOLY010000000
VERSION     DAOOLY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13485
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5016_length_13485_cov_4.987565"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PLD15_03060"
     CDS             complement(<1..1252)
                     /locus_tag="PLD15_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62909.1"
                     /translation="MKSMGEMLLSTGGGSEIVIGNTAIVRAMLEAGVRVATSYPGSPT
                     PEIASAIASIPRERNPMYFEFSVNEKVATEVAFGASINGHLSCVFFKSVGLNVAADTF
                     VQLSMMELIGGMVIVLGDDPGANSSQNEQDNRHYAHLSYTPVFEPATPSEVYVMFKQA
                     ADLSRKLRMPVIVRLSTHVCHAKEKVDFGPMPVHEHGNKPLFCMENGPYIPIAADALK
                     MKRRTLHKFWELKDLLEELPVNIVKDNSSERGVITAGAPYLSLMDVLEEVEDKPDVMK
                     LGIVHPLPKNRILQFLKAHKEVKILEELDDVLEREIKAFAYDNGLTTRILGKGDLEDW
                     IGEYTPDKVREVLSKCWPDTFKPVKEPDSPVKLNARPAQMCPGCGHRSAFHAIKLALK
                     DTDITVADIGCHTLGYLEPYRVGQV"
     gene            complement(1296..1694)
                     /locus_tag="PLD15_03065"
     CDS             complement(1296..1694)
                     /locus_tag="PLD15_03065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB62910.1"
                     /translation="MGSKVHSRVEFENTFKGTGKCDNGHTVPIGLGGAAPYDLLTMAL
                     SSCLYSTFLDIMAKKKIEFGGAEIDVAGEKRDEVPTTLKYCKVTVRVRSVTTDSKAGV
                     EKSFELATKYCSVYQTLSKVSQMDWEVIFE"
     gene            1851..2831
                     /locus_tag="PLD15_03070"
     CDS             1851..2831
                     /locus_tag="PLD15_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HPB62911.1"
                     /translation="MSLRPIFNPSLYHGESKKGNFFEGWYFKLVDRERKRSCAVIPGV
                     SLAGKAEKPHAFIQFLFGKEMRADNFTYDFDQFRYDKKRFDLHLGGSYFSAERISLDL
                     ESPGNRICGEVKFLESFKWPSSLISPGIMGPFTFVPFMECYHGVVSMDHLLDGRLEVN
                     GETWDFTGGRGYIEKDWGRSFPQAWIWMQSNSFDKPGVSFMLSVARIPWLGRSFTGFL
                     CGLLRDGELLRFTTYKMARIRTLEVSSDAVRLEIVMSRFLLKVFARRTGGASLLSPVR
                     GSMKGRINETLTSEISIELYEDDRPVFKGTGSDAGLEVVGELKAPEGGLR"
     gene            complement(3323..4105)
                     /locus_tag="PLD15_03075"
     CDS             complement(3323..4105)
                     /locus_tag="PLD15_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62912.1"
                     /translation="MKATLLLLFLIVCSLCLGWSFFTHSRIAVAALESIEPSLLEAIS
                     GGLPLPLFNDWIGLQASFVDVDRYFVRELVPGHSFRIHNYPGIEGLTMSEILELPGMS
                     RNALGALITAFFDVRNLSRSDDFAGTSYAIARLFHFASDLTMPLHVVNNNDGQLTGQP
                     GLHVRIESILGNFFFRSEMLLRQGPRIFGDEEIPAVLFESIRKSMEQSKVFEEADMKR
                     KRDSAYRFAALWGDVKEVFIEATSRAVYLCASLLISAISQPN"
     gene            4286..4819
                     /locus_tag="PLD15_03080"
     CDS             4286..4819
                     /locus_tag="PLD15_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62913.1"
                     /translation="MRSIKEKIEELDERVKAYSEEVEDDTVHLKVSNFLPDEQEIREI
                     IDNLEDGTRYLPLHRGVAVVVECEDAGTGDLDTFLESIKKELIPRLYYLDRDMKRLTR
                     IGLIMLAIGAVIMGVSTFLATMSFTASYLIDQILVITSWVFIWTSVEKIFFERRDVMY
                     HKRLIQRLYFASYCNVA"
     gene            complement(4944..5834)
                     /locus_tag="PLD15_03085"
     CDS             complement(4944..5834)
                     /locus_tag="PLD15_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPB62914.1"
                     /translation="MKKVILLVLMVGLLALTGMAAKTIGFAVSTLANPFFVTMKEGGE
                     AKAAELGLGFVVLDAQDSPEKQFSQVQDLIIRKVDVLIINPVDSDAIVPAVLEANAAG
                     IPVITVTRPSNGGVVAQHLDIDNKEAGMLAAVAMANALNGKGRVAILEGIPGAPSATD
                     RQQGFVNELKKYPDIQVVTSLTANYSREQGATVAEDILQGNPVLNGIYGHNDEMALGA
                     VRAAIAAGRLSELKIVGIDATDDALAAIKAGEMVATVQQQPALQMAMAVVAAQRIING
                     ATVEPKVIIPLKLITIEELE"
     gene            complement(5862..6830)
                     /locus_tag="PLD15_03090"
     CDS             complement(5862..6830)
                     /locus_tag="PLD15_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB62915.1"
                     /translation="MDKKIETGERKTVNWVKIKRLLKGELGLILILVALLAVFSFLSP
                     YFFTSKNLLNITRQVSITLIVAIGMTFVILTGEIDLSVGSAAALVGVTTAVVLKASGQ
                     LELAMIAGLLMGAGIGIVNGLLTVYGRVQSFIVTLAMLGVARGAALVWTGGKPISKLP
                     ADFGTMGAGYLGVVPVSSIIAAGIFVLAFFVLNRTKHGVYMKSIGANREAARLSAIPV
                     KRYRVLAFVISGVMSGLGGILITSRLLSAQPTAGEGLELNVIAAVILGGASLSGGIGT
                     VLGTLLGTMVIGVIDNGMNLVGVSSFFQQIVKGVIILVAVLAKRSD"
     gene            complement(6836..8338)
                     /locus_tag="PLD15_03095"
     CDS             complement(6836..8338)
                     /locus_tag="PLD15_03095"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB62916.1"
                     /translation="MIRVAGTVLKLEDINKSFGGIHALKNVQFELSEGEVHALLGENG
                     AGKSTLIKILTGVHRMDSGKILIHGKNVVIEDPVDARQKGIGAIYQELSLIDSLTVAE
                     NIFLGHEPVGNFFGNLKKKELTGRALEYLNTFGIEIAPETPVSDLGLGQKRIVEIVKA
                     LSINANILLLDEPTTGMSKAEIDTLFSIMDDLKSKKVTMIYISHYLDEVFRVCDRATV
                     LRDGENAGVFKIGEVDLKTLVRAMLGRTLKEEFPGRSGFPGRETVLEASEFRSEGMAA
                     PVSFKLHRGEILGITGIIGSGKSELALGLFGVDRKVAGEIAVKEERAAITSPNDAKRR
                     GISFVPEDRKVQGLFLRLPVEHNITIANLALSMKRGIISYRKRFEIARETAEKLKVTP
                     LDVKMPVQNLSGGNQQKVVIGKWLCGATDIFILDEPTRGIDIGAKTEIYSLINSLADN
                     GAGILIMSSEFREMSGVCDRVLIMRKGSVVSELAGEELTTEKILTVALGG"
     gene            complement(8335..9279)
                     /locus_tag="PLD15_03100"
     CDS             complement(8335..9279)
                     /locus_tag="PLD15_03100"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPB62917.1"
                     /translation="MIDFEFNGEILCMGSINMDLVMVMDRLPEPGETVVTDNFNTYPG
                     GKGGNQAVTAAVQGAKVKMFSKLGGDGFSKELLEKMASRGVDTSRILIDREKTAGIAI
                     IRVDGKGQNSISFTPGSNAFLTPGDVLENSDLFVPGRILLLTMEINLETIYRAIRLAG
                     QRGMFVIVDPAPAPSRPFPADIPPLVDVIKPNESEARAITGIEIKNTADAKAAIERLK
                     EMGFKLPVVTLGDKGVVACIDDGIVEFPPLSVECIDTTAAGDVFSGTLAASLAQGCSI
                     ERSLELANCAGALSTTIAGAQTSIPSPERVRQFMDKRV"
     gene            complement(9272..10120)
                     /locus_tag="PLD15_03105"
     CDS             complement(9272..10120)
                     /locus_tag="PLD15_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HPB62918.1"
                     /translation="MSDRKGILTEIFKVKKPIIGMVHLRPLPGSPGYDRSIMGMKEII
                     RIALEEARILEDNGVDGVQVENIWDFPYLKGEKIGPETAAALGVVAARVGETVGIPVG
                     VDCHLNGGRIALAAAIASGARWIRVFEWVNAYISHAGLTEGIGGELARYRHSLRADDI
                     KFMCDVNVKHGSHFIISDRTIEEQAHDAESEGAEILIVTGFETGKAPTPEKVKRFSES
                     VKAPVIIGSGLTEDNAQELLSFADGAIVGSFFKEGNDWKNPVDGKKVGSFMKKIHSLR
                     RELSDD"
     gene            complement(10231..10674)
                     /gene="rbsD"
                     /locus_tag="PLD15_03110"
     CDS             complement(10231..10674)
                     /gene="rbsD"
                     /locus_tag="PLD15_03110"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HPB62919.1"
                     /translation="MKTKGILHNDLSREVARLGHGDMLVITDRGFPFPRHENTVCIDV
                     SVGRNLPRLVDVVKVVLEELEIEKVIIADETKTVSPHTYAELKEVISKVKNKGNDIVE
                     ENIPHPEFKDLVLNGGLQGKEVKVFVKTGEFTPYANIILVSGVDF"
     gene            complement(10674..11696)
                     /locus_tag="PLD15_03115"
     CDS             complement(10674..11696)
                     /locus_tag="PLD15_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB62920.1"
                     /translation="MKDVAKLAGVSIATVSNVLSGNKYVSPELREKVLESIDRLQYRP
                     SKIARSLKIKKSFLVGLMVPDITNPYFAEIARGVESVALEHDYQMFLCNSDGEKSREE
                     ETLDSFHSHGVDGVINVAPRMEETILAELSNGMPMVILDRHLSIENPLIDVIYTNNFK
                     GSAQLARHFLENGHRRFACIAGPREVPTAVRRLQGFCNELEKSGIGKEEITVYYASFK
                     YEDGYNTMRLIMEQKPRPTAVFAGNDMMAWGAIEAAKERGLRVPEDIAVAGFDNVLYS
                     SLVVPAMTTVHQPKFEAGQTAMRLLLEKIQKKSRRESVYTRKIELDSNLIVRESTLSR
                     KIPEVN"
     gene            complement(11959..13446)
                     /locus_tag="PLD15_03120"
     CDS             complement(11959..13446)
                     /locus_tag="PLD15_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62921.1"
                     /translation="MKRLAMLLLLFLAVSLFSLDPYFTEENVNSFIAELEENGFVVQQ
                     GVVYTWDLLDLFSKHLIPSCYGNNASNPYLAYFLPPAPGQTVPNTLPFTFRLREDEAI
                     IFIGWTPPEVTYFSYVTFVMSKYLPGQSGRQRVLASVGDTINMTRIKTGESILPETAG
                     TVFNAPTMIISTPDKNMDVLMRAAAAEVGFIQSLINTEPIPSNMLKLGLEQDCDELLT
                     VIRLALFKNPEDKTYAQDPPLIVDGKLTVKPPYSHNYRGWVLRVTPTRPIEPDPFPTP
                     SMIPRDTGDYSELDLKPTMDRLEEAIIKEYSNLKADVLRTQRSVEISYMAIQNVTDVL
                     GDSRDTIYIWTDPFLIGDDPDDFAIVFGPVHSLTGKSTYSNFTVYTDDLVKDLLPVES
                     KILYGFASVHSEHSVESKLGLIGSAERFLPDDPNAKYFYVWKVARSNPDNEDYCLLIP
                     EPTSERLTYNNLRIAFRAYVNPETGVGPSYEEVLMDKVIHFSLDK"
BASE COUNT         2941 a         3729 c         3367 g         3448 t
ORIGIN      
        1 aaacctgtcc taccctgtac ggttcgagat agccaagcgt gtggcagcct atatcggcca
       61 ccgttatgtc ggtatctttc aaggccagct ttatcgcatg aaaggccgaa cggtgtccgc
      121 agccgggaca catctgggcc ggtcgcgcgt tgagtttgac aggactgtcc ggctccttca
      181 cgggcttgaa ggtgtcgggc cagcactttg aaagtacctc cctgactttg tcgggcgtgt
      241 actcccctat ccagtcttcg agatctcctt tccccagtat tcttgtggtg agtccgttgt
      301 cgtaagcgaa ggcttttatc tctctctcga gaacatcgtc gagctcttcg aggatcttca
      361 cttctttgtg agccttcaga aactgtaaga tcctgttctt cggtagcgga tgaactatgc
      421 ccagcttcat aacgtcgggt ttgtcttcca cctcttccag cacgtccatg agggaaaggt
      481 acggcgctcc ggccgttatt acgcctcttt cgcttgaatt gtccttcaca atgttgacgg
      541 gaagctcttc gagcaggtcc ttaagctccc agaatttgtg gagggtgcgt ctcttcattt
      601 tcagcgcatc ggccgctatc ggtatgtagg ggccgttttc catgcaaaag agcggtttgt
      661 tgccgtgctc gtgaacgggc atcggcccga aatcgacctt ctctttcgcg tggcagacgt
      721 gggtcgaaag cctcacgatg acaggcattc tcaacttcct ggagagatcg gcggcctgtt
      781 tgaacatcac atagacttcc gaaggcgtag ccggttcgaa gacaggggtg taagaaagat
      841 gcgcgtaatg cctgttgtcc tgttcgttct gcgaggagtt ggccccgggg tcatcaccca
      901 gaacgatcac catgccccct atcagttcca tcatggacag ttgtacgaaa gtgtcggcgg
      961 cgacgttcag cccgacgctc ttgaaaaaga cgcacgagag gtgcccgttg atagaagcgc
     1021 caaaggccac ctcggtggcg accttctcgt ttaccgagaa ttcgaaatac atcggatttc
     1081 tctctcttgg aatggaggct atcgcgcttg ctatctcggg agtgggcgaa ccgggatatg
     1141 aagtggcaac ccgcacccct gcctcgagca tggccctgac gatggccgta ttgcctatca
     1201 ctatctcgga gccgcctccg gtggagagaa gcatttcacc catgctcttc attgtaatcc
     1261 cgcctttccc gttatttggt ttatccgacc gattttcact cgaagatcac ttcccagtcc
     1321 atttgagaaa ctttcgacag tgtctggtaa acagagcagt atttggtagc cagttcgaag
     1381 gacttttcca caccggcctt actgtcggtt gtcacgctcc tcactctgac ggtgactttg
     1441 caatatttca gagtcgtggg tacttcgtcg cgcttttcgc cggcgacatc gatctcggcg
     1501 ccgccgaatt ctatcttctt cttggccatg atgtcgagaa aagtcgagta tagacacgag
     1561 gaaagggcca tggttagaag atcgtacgga gcggcgcctc ccaatccaat tggcaccgta
     1621 tgcccgttgt cgcactttcc cgtgcctttg aacgtgtttt cgaactccac tctggagtga
     1681 actttgctgc ccatttttct cctccttctc catctgtcgt ttcttctcgc aatgtattat
     1741 cgttataaat attagcattt cgatacactg tcagtccaga caactgtact caacgcctct
     1801 tctttgcgca tgaaatatgt aaaattatta tatcgtggag ggggaataat atgagcttga
     1861 gaccgatctt caatccatcg ctataccatg gcgagtcgaa aaaggggaat ttcttcgagg
     1921 gctggtattt caagctcgta gatcgagaga gaaagagatc gtgcgccgtg ataccgggag
     1981 tatcgctggc cggaaaggca gaaaagcccc atgccttcat tcagtttctc ttcggaaaag
     2041 agatgagggc ggacaacttc acttacgatt tcgaccaatt ccgctacgat aagaagcgct
     2101 tcgatctaca cctgggaggg agctacttct ccgcggagag gatctcgctg gacctggaga
     2161 gtccgggaaa ccgcatatgc ggagaagtaa agtttctcga atccttcaaa tggccctcga
     2221 gtcttatttc cccgggcatc atggggcctt tcacattcgt gcccttcatg gagtgctatc
     2281 acggggtcgt gagtatggat cacctcctcg atggccggct cgaggtaaac ggcgaaacgt
     2341 gggacttcac cggtggcagg ggttatatcg aaaaggactg gggaagatcc ttcccgcaag
     2401 cctggatatg gatgcagagc aacagcttcg ataaaccggg tgtttcgttc atgctttccg
     2461 tagcgaggat tccctggctt ggaaggagct ttaccggctt tctgtgcggt ctgctgcgcg
     2521 atggcgaact tcttcgcttt acaacctata aaatggccag aattcgcacc ctcgaagtct
     2581 ccagcgatgc cgtgcgctta gagatagtca tgtcgcgttt cctcctgaag gttttcgcac
     2641 gcagaacagg aggggcgtcc ctcctgtcgc cggttcgcgg gagtatgaag gggagaatca
     2701 acgagaccct gacctcggag atctctatcg agctgtacga agacgaccgg cctgtcttta
     2761 aaggcacagg gagcgacgcc ggtctcgaag tagtgggcga gttgaaggcc cctgagggag
     2821 gacttagata gaactagagc ggccgatgca ccgcttcacg tacctacgaa atctcccgca
     2881 ccgtcatccc gtagtggtcc catcacgaga agacgagagc gaaaaacacg agagaaagaa
     2941 catcgagagt tcgcttcgct cacgagaagg cgtctctctc tctcttctct ctccttctct
     3001 ctcttcctct ctcttcctct ctctttctct ctactcttct cgctctttcg gagtctgtcc
     3061 catttttcgc gtccgtcctc tcgcgttcgg gttctgggat ctaggatcta gggtctagta
     3121 agagcgagat cccgtataga tgctctacgg gatgacagtc ttactccctt accgtcatgc
     3181 cggaccccga tccggcatct cgtctctgga gagaccagac ccaggaacca agagttctac
     3241 tatgtcttcg gtcttggacc aagaaactga acgcgtggcg ttgaggaacc gctcttcgca
     3301 actatagata gctttcttct ggtcagttgg gctgcgaaat cgcgcttata agaagacttg
     3361 cgcatagata tacggctctg ctcgtggctt cgatgaatac ttcttttacg tccccccaga
     3421 gtgcggcaaa cctgtaggcg gagtctctct ttcttttcat gtcggcttct tcgaatactt
     3481 tagactgttc catcgacttg cggatacttt cgaaaagcac cgcggggatc tcctcgtctc
     3541 cgaagatgcg cggtccttgc ctgaggagca tttcgctcct gaagaagaag ttgcccagaa
     3601 tcgattcgat cctcacatgg agtcccggct gaccggttaa ttgaccgtcg ttgttgttca
     3661 caacgtggag cggcatcgtg agatcgctgg caaagtggaa aagcctggcg atggcgtagg
     3721 aagttccggc gaagtcgtcc gatcgggaga gatttctgac atcaaagaag gcagttatga
     3781 gggcgccgag ggcgtttctg gacattccgg gaagctcgag aatctctgac atcgtcagcc
     3841 cttctattcc aggataatta tgaatacgga agctgtgacc gggaacgagc tccctcacga
     3901 agtacctgtc aacgtcgacg aaagatgcct gcagccctat ccagtcgttg aaaagcggca
     3961 ggggcagacc cccgctgatc gcctcgagca acgaaggctc gatgctttca agagccgcga
     4021 cggctatcct gctgtgggtg aagaagctcc atcccaggca aagagagcat actatcagaa
     4081 atagcagtag aagagtcgcc ttcataattc actccgtgca gggccaaacg cccggtatga
     4141 ccaatggtaa catagatctt tcgtgtactc gattgaaatt aagtgagtac accgtgaaag
     4201 aaaaagcttt ccggcctgtc tcgaatgata tactctcgat gaaaactgtg gaaagggggg
     4261 aaacaacata agcagaaagg tacccttgag atccataaaa gagaagatag aagagctgga
     4321 tgaaagagtt aaggcctatt ccgaagaggt agaagatgat acggttcatt tgaaagtctc
     4381 gaacttcctc ccggatgagc aggaaattcg cgaaatcatc gacaacctgg aagatggaac
     4441 ccggtatctc cctctccaca ggggagttgc ggtagtcgta gaatgtgaag atgcgggaac
     4501 gggcgatctg gatacctttc tagagtccat aaagaaagaa ctgattcccc ggctgtatta
     4561 cctggatagg gatatgaaga gactcaccag aatcggcctc ataatgctcg ccatcggcgc
     4621 cgtcatcatg ggagtctcca cttttctcgc gacgatgagt ttcacggcct cgtacctgat
     4681 cgaccagata ctggtcataa caagctgggt cttcatctgg acttcggtgg agaagatctt
     4741 cttcgaacgg cgcgatgtta tgtatcacaa gcgccttatc cagagactgt attttgccag
     4801 ttactgtaat gtagcttgag cgaggattta ggatcaggag cccggaaact cacagtatga
     4861 gttatgcggg ttatgagtcc cgtatttcct gcacaaaaaa acgggcgcct ttgaggacgc
     4921 ccgtcttttc gattaaccct gaattattcc agttcttcga tggtgataag ctttagcgga
     4981 attatgacct taggctcgac ggtcgcgccg ttgattattc tctgcgccgc cacgacggcc
     5041 atagccatct gtaaggcagg ctgctgctga accgtggcga ccatttctcc ggctttgatg
     5101 gcagccaggg cgtcgtcggt ggcatcgatt cccacgattt tcaattcgga aagtctgccg
     5161 gccgcgatcg cagctctcac cgcaccgaga gccatttcgt cgttgtgtcc gtaaatgccg
     5221 ttgagcacag ggttgccctg aaggatatcc tcggcgacag ttgcgccctg ctctctcgag
     5281 tagttcgcgg taagggatgt cacaacctgg atatccgggt atttcttgag ttcgtttaca
     5341 aagccctgct gtctgtcggt ggccgatgga gccccgggga ttccctcgag aatggcgacc
     5401 cttcccttac cgttcagagc gttagccatg gcaactgcgg ccagcattcc ggcctctttg
     5461 ttgtcgatat ccaggtgctg tgcgacgact ccgccgttgg acggcctggt aacggtaatg
     5521 acaggtatgc ccgcggcgtt ggcctcgaga acggccggaa ctatcgcgtc ggaatcaacg
     5581 gggttgatta tgaggacatc gacttttctg attatcaggt cctggacctg agagaactgc
     5641 ttctccggag agtcctgcgc atccagaacg acgaagccca gaccgagttc ggcggccttt
     5701 gcttcccctc cctccttcat ggttacgaag aaaggatttg ccagagtaga gacggcgaag
     5761 ccgattgtct ttgccgccat gccggttagt gccaacaatc ccaccatcag aactaaaaga
     5821 attaccttct tcatgttact cctccttttc ttttcgaacg atcagtcact gcgtttggcc
     5881 agtactgcaa caaggattat tacacccttt acgatctgct gaaagaacga cgatacaccc
     5941 accagattca ttccgttgtc tattacaccg atcaccatgg tacccagaag cgtgccgaga
     6001 accgttccta taccccctga cagggaagcg ccacccagaa ttacggcagc gatcacgttc
     6061 agttcaagtc cttcgccggc ggttggctgg gccgaaagga gtcttgaagt gatcaatata
     6121 ccgcccaggc cgctcatcac gcccgatatc acgaaggcga gaaccctgta gcgcttgacg
     6181 ggaatggccg acagcctcgc cgcttcgcgg ttggccccta tcgatttcat gtaaacaccg
     6241 tgcttggttc tgttcagaac gaaaaacgcc agtacgaaga ttccggcggc gatgatggat
     6301 gagaccggaa cgacccccag gtatccggcg cccatggttc cgaaatcagc gggcaacttc
     6361 gagatcggtt tcccgccggt ccagacaagg gccgctcctc ttgccacgcc cagcatcgcg
     6421 agcgttacga taaacgattg aaccctaccg tacaccgtta gaagtccgtt aacgattccg
     6481 attcccgccc ccatgaggag gcctgcaatc atagccagtt ccagttggcc ggacgctttc
     6541 aagactaccg ccgttgtaac cccgaccagc gccgcggccg atcctaccga gagatctatc
     6601 tctccggtga ggatcacgaa agtcatgcca atagcgacta ttagcgtgat cgaaacctgc
     6661 cgggtgatat tcagtagatt cttcgaggtg aagaaataag gggacagaaa ggagaagacc
     6721 gccagcaggg ccacgagtat cagtatcaat cccagctcgc ccttcaggag tcttttgatc
     6781 ttcacccagt tcacagtctt tctctccccg gtttctattt ttttgtccat cacgttcaac
     6841 ctccaagcgc cactgtaagt attttctccg ttgtcagttc ttcaccggcg agttcggaaa
     6901 ccacgcttcc tttacgcata atcagtaccc tgtcgcacac tccgctcatc tctctgaact
     6961 ccgaggacat tatcagtatc cccgcgccgt tgtcggccag ggagtttatc agagagtata
     7021 tttccgtctt cgcaccgata tctattccgc gggtcggttc atcgagaatg aaaatatcag
     7081 tcgccccgca aagccacttc cctataacca ccttttgctg atttccgccg gaaaggttct
     7141 gcacgggcat ttttacgtcc aggggtgtga ctttgagctt ttcggccgtt tctctggcga
     7201 tctcgaagcg cttcctgtag gaaataattc cccgcttcat ggagagcgcc agattggcga
     7261 tggtgatatt gtgctcgaca ggtagtctca agaagaggcc ctgaaccttt ctgtcctccg
     7321 gcacaaagga gatgccgcgc cttttagcgt cgttcggcga tgttatcgcc gccctttcct
     7381 ccttcaccgc tatctcgccc gccacctttc gatcgactcc gaacagaccg agtgccagct
     7441 cgctcttgcc cgaacctatg atccctgtga tcccaagtat ttcgcccctg tgaagcttga
     7501 aggaaaccgg tgcggccatg ccctcgctcc ggaactcgct ggcttcgagc acggtctccc
     7561 ggccggggaa gccgcttcgc ccggggaact cctctttcaa ggttctaccc agcatggccc
     7621 tgaccagggt tttgagatcc acctccccga ttttgaagac gccggcgttt tcgccgtccc
     7681 tcagaacggt ggccctgtcg cagaccctga agacttcatc cagatagtga gagatataga
     7741 tcatggtgac tttcttactc ttcaggtcgt ccattatgga gaagagagtg tcgatctcgg
     7801 ccttgctcat tccggtggtc ggctcgtcca gaagaagtat gttggcgttg atcgagagcg
     7861 ctttcacaat ttccacgatc ctcttctgtc ccagtccgag gtccgatacc ggcgtttccg
     7921 gcgctatttc tatgccgaag gtgttgaggt actccagagc ccttccggtc agttccttct
     7981 tcttgaggtt gccgaagaaa ttgccgaccg gttcatgacc caggaagata ttctcggcca
     8041 ccgtcagact gtcgattagg ctgagttcct ggtagatagc ccctatcccc ttctggcgag
     8101 catcgacggg atcttctatc acgacgttct tcccgtgaat caggatcttt cccgagtcca
     8161 tcctgtggac accggtcagg atttttatga gcgtcgattt tcccgcaccg ttctcgccca
     8221 ggagggcgtg aacctcgccc tcggagagtt cgaactgaac gttcttgagc gcgtggatcc
     8281 ctccaaagga tttatttata tcttcgagct tcagtacggt tccggccacc cttatcacac
     8341 ccgcttatcc atgaattgcc ttactctctc aggggacggt atcgaagtct gggctccagc
     8401 gatcgtggtg ctcaaggctc cggcgcagtt ggcaagctca agcgatctct cgatcgaaca
     8461 gccctgcgcg agcgaggcgg cgagagtccc tgaaaacacg tcgccagccg ccgttgtgtc
     8521 gatgcattcc acgctcaagg gggggaactc tacgattccg tcgtcgatgc aggccaccac
     8581 gcctttatct cctagtgtta ccaccggcag tttgaaaccc atctctttca atctctctat
     8641 cgcggccttc gcatcggccg tgttttttat ctcgattccg gtgatcgccc tggcctcgga
     8701 ttcgttcggc ttgataacat ccaccagagg agggatatcg gcggggaagg gtctggaagg
     8761 tgccggtgcc ggatcgacga tcacgaacat cccgcgctgg cccgccagcc ttatggcccg
     8821 gtatatggtc tcgagattta tctccatcgt caggagcagg atcctgccgg gcacgaagag
     8881 gtccgagttt tcgagtacat cgcccggcgt cagaaaggcg ttgctcccgg gggtgaagga
     8941 tatcgagttc tggcctttgc cgtccacacg tatgatagcg ataccggccg ttttttccct
     9001 gtctatgagt atcctggatg tgtcgacacc cctggaagcc atcttctcca gcaactcctt
     9061 gctgaaaccg tcgcctccca gttttgaaaa catttttacc ttcgcaccct gcacagcggc
     9121 cgtaacggcc tggtttccac cctttccgcc gggataggtg ttgaagttgt ccgtcaccac
     9181 agtctcgccc ggttcgggaa gacggtccat aaccatcacc agatccatgt ttatactgcc
     9241 catgcagagt atctcgccgt tgaattcgaa gtcaatcatc cgaaagctcc cttctcagag
     9301 aatgaatctt tttcatgaaa ctcccgactt ttttaccgtc cacgggattc ttccagtcgt
     9361 ttccttcttt gaaaaaactt ccaactatcg cgccgtcggc gaaagataga agttcttggg
     9421 cgttatcttc cgtcagtccg ctgccgatga tcaccggtgc tttcacactc tcggagaatc
     9481 tcttcacctt ttccggagtc ggggcctttc cggtttcgaa tccggtgacg atgagaatct
     9541 ccgcgccctc cgactccgca tcgtgggcct gttcttcgat cgtcctgtcg gatatgatga
     9601 aatggctccc gtgcttgacg ttgacatcgc acatgaactt gatatcgtcg gctctgagcg
     9661 agtgcctgta cctggccagc tccccgccta tcccttcggt gagcccggcg tgcgaaatgt
     9721 aagcgttcac ccattcgaag acccttatcc atctcgcgcc cgaagcgatg gccgctgcca
     9781 gagctatcct gccgccgttg agatggcaat ccaccccgac gggaataccg acggtctccc
     9841 ctactctagc ggcaaccaca ccaagcgccg cggcagtttc cggaccgatc ttttctccct
     9901 tcagataagg aaagtcccat atgttttcaa cctgcactcc gtcaacgccg ttgtcctcga
     9961 gtatcctggc ctcctccagg gcgattctga ttatctcctt catgcccatg atcgaccgat
    10021 cgtagccggg agaacctggc aaaggtctga gatggaccat cccgataatt ggttttttaa
    10081 ctttgaagat ttcggtcagt attcccttcc tgtcgctcac actcttccac ctcccggcta
    10141 aacacacaaa tcgacagaga tgccggataa acgtttaact acgaccgcgg cgatcgaccc
    10201 gtccaatcgt ccaatcgtcc gatcctgctc tcagaaatcc actccggaaa ccagtattat
    10261 gttggcgtaa ggtgtgaatt caccagtctt gacgaagacc ttaacttcct tgccctggag
    10321 tcccccgttc agtaccaggt ccttgaattc cggatgggga atgttctcct caacgatatc
    10381 gtttcccttg tttttcacct tcgaaatcac ttccttgagc tcggcgtagg tatgcgggct
    10441 cactgttttt gtctcgtcgg cgattatcac cttttcgatc tccagctcct ccagaaccac
    10501 cttcacgacg tccaccaagc gcggtaagtt cctgcccacc gacacatcga tgcaaacggt
    10561 gttttcatgc ctggggaagg ggaagcccct gtcggttatt accagcatgt ctccatgacc
    10621 gagcctcgca acttctctgg aaagatcgtt gtgcagtatc cctttcgttt tcatcagttc
    10681 acctccggta tttttctgct aagggtcgat tccctcacta taagatttga atctagctct
    10741 atctttctcg tatatacgct ctcccgtctg gatttcttct gtatcttctc cagtagaagc
    10801 ctcatggcag tctgcccggc ttcgaacttc ggctggtgca cagtggtcat agccggaacg
    10861 accagcgacg agtaaagcac gttatcgaaa ccggccaccg cgatatcttc gggaaccctc
    10921 agccctcttt ccttcgccgc ctcgattgca ccccaggcca tcatgtcgtt tccggcgaaa
    10981 acggcggtgg ggcgtggctt ttgttccata atcagcctca tggtgttgta gccgtcttcg
    11041 tacttgaaag aggcgtaata aacggttatc tcctcttttc cgatgccgga cttctcgagt
    11101 tcgttgcaga aaccctgaag cctcctgacg gccgtcggca cttccctggg accggcgatg
    11161 caggcgaacc tgcggtggcc gttctccaga aaatgccttg ccagctgcgc cgatcccttg
    11221 aagttgttcg tgtagatgac gtctattagc ggattctcga tagacagatg ccgatcgagg
    11281 atcaccatgg gcataccgtt ggaaagctcg gcaaggatcg tctcttccat tctcggagcg
    11341 acgtttatta cgccatcgac gccgtggctg tgaaaggaat cgagggtctc ctcttctctg
    11401 gacttttctc cgtcgctgtt gcagaggaac atctggtaat cgtgctccag ggccacgctt
    11461 tccacgcccc tggctatctc tgcaaaatac gggttggtta tgtctggaac catgagaccc
    11521 accaggaaag atttctttat tttgagactc ctggcgatct tgcttggtct gtattgaagg
    11581 cgatctatgg actcgagaac cttttcgcgc agctcggggc tcacatattt gttcccggaa
    11641 agtacgttgg aaaccgtcgc gatagagacc cccgcaagtt tggctacatc tttcatatta
    11701 gacaaatggc tcacccgttt catagttaaa cgtttagtta atcgtttaac aagagaatac
    11761 tacttctcct cgctctctgc aaaatccatt taacgccttt cagagacgtt cataaaagaa
    11821 gaagagcgat tatcaaatcc tcgctcttct tcccaaagct tttcgcgcct atctttatat
    11881 ttcaacgtat gctattcttg cggaattatc cacctacatt cccgcattac ccagcggtcc
    11941 cgcatagttt ttgcgatgct atttatcgag actgaagtgg atcaccttat ccatgagaac
    12001 ttcttcatac gatggcccta ctccggtctc aggattcaca taagctctga acgctattct
    12061 caggttgttg taggtaagcc tctcagaagt aggttctgga atcaacagac aataatcttc
    12121 attatccggg tttgacctcg caactttcca gacatagaaa tacttcgcgt ttggatcgtc
    12181 gggcagaaat ctctctgcgc tgccaataag gccaagtttg ctctcaacgc tatgctcact
    12241 gtgaacactc gcgaatccat agagaatctt tgactcgact ggcagaagat ccttcacgag
    12301 atcatctgta tagactgtga aattcgagta agttgacttt cccgtaagcg agtgtaccgg
    12361 accaaacact atggcgaagt catcgggatc atctcctatc aggaagggat ccgtccagat
    12421 atatatagta tccctgctat ctccaagaac atcagtgacg ttctgaatgg ccatgtaact
    12481 aatctccaca gatctttgag tccgaagaac gtcggccttt agattggagt actctttgat
    12541 tattgcttcc tccagtctgt ccatagttgg ctttaggtcg agttcagaat aatcgccagt
    12601 gtcccttgga atcatgctcg gcgtcgggaa tggatcaggt tcaatcggtc tggtaggtgt
    12661 gacgcgcaag acccagcctc gataattatg actgtaagga ggcttcacgg tcagtttgcc
    12721 atccactata agtggaggat cctgtgcgta ggtcttgtct tcgggatttt tgaacaatgc
    12781 caggcgaatc acagttagta actcgtcgca gtcctgctca agccccagtt tcagcatatt
    12841 tgaaggaatc ggttcggtgt tgatcaggct ctgaataaaa cccacttctg ccgcggcggc
    12901 tctcatcagt acatccatat tcttatcagg ggtagatatg atcatcgttg gagcattgaa
    12961 aacagttccg gccgtctcgg gtaggatcga ttcgcccgtc ttgattcttg tcatattgat
    13021 cgtatcacct acacttgcca ggacacgttg ccggccgctt tgaccgggca gatacttgga
    13081 cataacaaac gtgacatagc tgaagtaagt gacctccggt ggagtccagc cgatgaagat
    13141 aatcgcctcg tcctccctta gcctgaaggt gaacggcaag gtgtttggaa ctgtctgccc
    13201 gggcgccggc ggcaagaaat atgctaggta gggattggaa gcgttgtttc cataacagct
    13261 cgggatcagg tgttttgaaa agaggtctag cagatcccaa gtgtaaacca caccctgttg
    13321 tactacaaac ccgttctcct caagctctgc gatgaagctg tttacgttct cttcagtgaa
    13381 gtatggatcc agcgaaaaca atgagacggc aagaaatagc agaagaagca tcgccaaacg
    13441 tttcattctc ggttcccccc tgggttccat ttacccacat tagtg
//
LOCUS       DAOOLY010000036        13404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5064_length_13404_cov_4.355457, whole genome shotgun sequence.
ACCESSION   DAOOLY010000036 DAOOLY010000000
VERSION     DAOOLY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5064_length_13404_cov_4.355457"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1458)
                     /locus_tag="PLD15_03125"
     CDS             complement(94..1458)
                     /locus_tag="PLD15_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HPB62922.1"
                     /translation="MKVQILTAEIGSTTTVLSAFSCDASGKLSFLAQGESYTTVAEND
                     VTVGIERALIELKKRLNDDRLDWEVFIASSSAAGGLRMTVHGLVYDMTVRAAKEAALG
                     AGGVIKLVTAGKLSKRDLEKISRIQPRLVLLAGGVDYGEEETVIHNAGMINQLDGSIP
                     LVYAGNTAVSDEVREIISPTGREMHFVDNVYPRIDELNVEPTRKIIREVFSRNIIKGP
                     GMEKIYSIANKPVIPTPAAVMLAAELFSKVYNDVLVVDIGGATTDVDSVTDGDPEISK
                     MLVSPEPRSKRTVEGDLGVYVNARHVIDHIGEKELRGEFAYFDEIVGSLSPYPVNDEA
                     SRFSARLGRYCFETSIRRHAGYIRQMYGPTGRIEVAQGKDLTAIKSIVGTGGLLSRSR
                     YTRQIIDNAKSLSGNHSKELLPGPDVRIYRDRNYIFAAIGLISTVDASLATDLIDSAL
                     EELR"
     gene            complement(1455..1916)
                     /locus_tag="PLD15_03130"
     CDS             complement(1455..1916)
                     /locus_tag="PLD15_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPB62923.1"
                     /translation="MSLYEKIQSDMKEAMKLRDTLRTNTLRSVIAAVKNFQASSEENR
                     KAEVTDDTVINLIGKEVKRREESIEAYIKAGRDELVAQETLEMEILKDYLPEEMGEDE
                     IRAVALKTIEDLKANGPSDLGKVMREVMVRLKGRADGKLTNKIVRELLESR"
     gene            complement(1922..3430)
                     /gene="lysS"
                     /locus_tag="PLD15_03135"
     CDS             complement(1922..3430)
                     /gene="lysS"
                     /locus_tag="PLD15_03135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPB62924.1"
                     /translation="MSEETRRQRLREIDQMREEGVEPYPYRFEKTHWAGDIKNEFSTL
                     ENSQTKEDVEIRTAGRVMALRSHGKSSFFVLRDDTGRIQAYIRLDSVGKDSFEIFKKY
                     VNIGDFVGVTGFPFKTHTGEVSVFAKSFEILSKSIRTMPEKWHGLKDKEIIYRQRYAE
                     MLSSDEALNRFKIRSELFRLIREFLAQRRFIEVDTPMLLHLTGGASARPFETRINVFE
                     SQMFLRIAEELFLKRYLVGGFERIFEIGKNFRNEGISYKHHPEFTMMELYWAYADYND
                     IMDITEAMINHIVENLAGSTRISYQGREIDFSRPWRRVKMADFIQDNLGANILEDSDE
                     RLIEVLKKHNSEPDMKERGHLIEKLWDLVEDKLVNPTFVTEHPVIISPLSKVHRSDPR
                     VTERFELIISSMEMANAFSELNDPIEQHRRFLHQAGLREAGDEEAQMMDDDFLRALEY
                     GMPPTGGLGIGWDRILMLVTDSPTIRDIIPFPLVRPISFEEEELQSEEELQE"
     gene            complement(3443..3916)
                     /gene="greA"
                     /locus_tag="PLD15_03140"
     CDS             complement(3443..3916)
                     /gene="greA"
                     /locus_tag="PLD15_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPB62925.1"
                     /translation="MKKKAIYLTQEGYDRMKEELENLRKRLMYDIAERIKEARELGDL
                     SENSEYDEAKNEQGRIDSRIKQLEEILNNAEVIEDDGDMSTVKLGHIVELQNMENGEK
                     SEYRIVNAQEANIFEGKISSDSPIGKGILTHKVDEVVRVKTPSGWAKYKILTIGK"
     gene            complement(3944..4396)
                     /gene="nrdR"
                     /locus_tag="PLD15_03145"
     CDS             complement(3944..4396)
                     /gene="nrdR"
                     /locus_tag="PLD15_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPB62926.1"
                     /translation="MKCPFCSSESTRVLDSRPTEDNTSIRRRRECEDCGGRFTTYERY
                     ERLPFFVIKKDGRRERFNREKIMNGLLRACEKRPISLERLSSIVDSIEDEVTRSGRHE
                     VLSQEIGEMIMAKLKLLDRVAYVRFASVYKEFRDIDHFMDIIKELKND"
     gene            complement(4396..5322)
                     /gene="whiA"
                     /locus_tag="PLD15_03150"
     CDS             complement(4396..5322)
                     /gene="whiA"
                     /locus_tag="PLD15_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPB62927.1"
                     /translation="MSYADKLKEELCHLSTEDTSESRAEFLGYVKSRGTLRIRDGTSY
                     LVILLTSITSLKRLFQITRRLSLPIYETQVTEEIRFSRKRGGELSYIFADVEKFLESN
                     GISVRNDIIPSPVRDDPVYFGAFVRGLYLAGGSIVDPSKEYHLEITLDTTGEFVDSLR
                     DYLHDNFNIKCGVVKVRNKFKVYVKSAGDLMELLSLMGGKRTVAHLSRAVEVRKIRGN
                     VSRTLNFLTANANKSGQAMARHVRAIRIIDAKIGLARLDSELKQVAILRLENEDLNLR
                     ELGEMMNPPMSKSAVYNRLKKLISIAEEMGEI"
     gene            complement(5319..6281)
                     /gene="yvcK"
                     /locus_tag="PLD15_03155"
     CDS             complement(5319..6281)
                     /gene="yvcK"
                     /locus_tag="PLD15_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HPB62928.1"
                     /translation="MRKIVLIGGGTGLSTFARVLKGFPVELTLVVAITDDGGSSGILR
                     EEMKIPPPGDIRNNIIALANDEEILTKVFSHRFSTDAMKKHSLGNIIIAGLTEMYGSF
                     PDAVIAASKLLNIKGRVLPVADALVRLIGELEDGSKVEGESAVARYGKKIKRLTLDRK
                     VQALPEVLRAIETAETLIVGPGSLFTSVVPNFLVSGVSEAFSRSPGKRVYICNIMTQP
                     NESEGFDLKKHVDTVESYTGTPFDRVFWTEVGGVEKAVLERYRRAGAAPVMNDLLDDT
                     RVTSICGTTTELITDGREKLVVRHSSESVVSILRELEIFEEILL"
     gene            complement(6290..7105)
                     /gene="murI"
                     /locus_tag="PLD15_03160"
     CDS             complement(6290..7105)
                     /gene="murI"
                     /locus_tag="PLD15_03160"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPB62929.1"
                     /translation="MKDNLKIGVFDSGIGGLTVLKKLLQGFPRGVDFFYFADTARVPY
                     GSKPIETLRGYIGEIFDFFFQLGIDAIVTACNTSDSVLSQREKQNLPVPYFSIIDPTV
                     RTLGSIAPKGSEVSVIATSNTVRRSLYLRKLFRYENLRRITQKACPLFVPIIEEGIWE
                     GEIVDAVVSYYLAEINRMEPDYLILGCTHYPFIRKVIESSISRKTLLIDPADFILVDL
                     INWIGEIDGEESRVTYYVSGNPESFQRVLKRYFQREGDVVPVDLVNERITVKR"
     gene            complement(7095..7376)
                     /locus_tag="PLD15_03165"
     CDS             complement(7095..7376)
                     /locus_tag="PLD15_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPB62930.1"
                     /translation="MKRSVSLNLGGRQFSFLSGDPQEVVDQVFSKVTEMYDAFKKKEE
                     EIGFEKLMVGICVNLAHDLIKSQNELVRLKAKYEEVLSEYFQGREGVEG"
     gene            complement(7389..7625)
                     /locus_tag="PLD15_03170"
     CDS             complement(7389..7625)
                     /locus_tag="PLD15_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62931.1"
                     /translation="MDSYQELELVVDKMVEEFSRLKKENEQLWKQVQQSQQKLEEAGL
                     KIRELELRLESESKTISSLIKRVKVSLDENSKKT"
     gene            complement(7719..8468)
                     /gene="tpiA"
                     /locus_tag="PLD15_03175"
     CDS             complement(7719..8468)
                     /gene="tpiA"
                     /locus_tag="PLD15_03175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB62932.1"
                     /translation="MVAGNWKMNKSPGEARMFAGFLASSIGEESSVDVVVFPTSLAVS
                     GVADMMKDTKIKVGVQNIHPADGGAFTGEISVPMLKELYIDYVLVGHSERRQLFGETD
                     AQTNEKIKAVLRNGLTPVFCIGETLEERKANLTNSVLERQLKNGLMGFTKEEASGVII
                     AYEPVWAIGTGVVATPEQAEETMKYIRQFLVGLYDYDLAESIRILYGGSIKPDNFDSL
                     VAMEDIDGGLVGGASLLESFVQLVAIAKNHE"
     gene            complement(8468..9685)
                     /locus_tag="PLD15_03180"
     CDS             complement(8468..9685)
                     /locus_tag="PLD15_03180"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB62933.1"
                     /translation="MAKKLTLKDVELKGKKVLVRVDFNVPLDKTTGEVTDDTRIAAAI
                     PTIEYITGKGGKAILVSHLGRPKGGKDPKYTLKNVASRLEKLIGKPVKFVDDCIGPEV
                     EKAVSDMKDGEILLLENVRFYPEEEKNNPEFAAKLAAFADIHVNDAFGTAHRGHASNT
                     GVAAHLVSIAGFLMEKEVEMLGMAIENPEHPYVVILGGAKVSDKIGVITNLLEKADRI
                     LIGGAMMFTFLKALGRNVGDSLVEDDKIELAREILDKASRKKVDFVLPVDAVTAREIA
                     AGVEKKVVMTDEGIPAGWKGLDIGPKTVELFKSKLADAKTVVWNGPMGVFEIDDFANG
                     TETVAKALATLKSAVTIIGGGDSAAAINKFGLAGEVSHVSTGGGASLEMLEGREMPGI
                     ESLANATGKKNED"
     gene            complement(9747..10742)
                     /gene="gap"
                     /locus_tag="PLD15_03185"
     CDS             complement(9747..10742)
                     /gene="gap"
                     /locus_tag="PLD15_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB62934.1"
                     /translation="MHKVAINGFGRIGRLVFREMMARGGFDIVAINDLTDAATLAHLL
                     KYDSVHGRFKGTVQAKEGAIVVNGKEIKVFAEKSPTNLPWKALGVELVIESTGVFTSK
                     EKTMPHVEAGAKKVLITAPAKGAVDATIVLGVNDDELKPEMQVVSNASCTTNSIAPII
                     KVLNDNLKIVKGYLTTVHAYTNDQRILDLPHKDLRRARAAAANTIPTSTGAAKAVGLV
                     IPGLKGKLDGIAMRVPVTDGSITDLTVIVEKETTAEEVNALIKKAAENELKGIIEYSE
                     EELVSSDIIGTTVSGVLDGKLTSAMGNLVKVCAWYDNEYGYSCRVVDLAEKMMKM"
     gene            complement(10755..11120)
                     /locus_tag="PLD15_03190"
     CDS             complement(10755..11120)
                     /locus_tag="PLD15_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPB62935.1"
                     /translation="MAKKLRGLGGRNYGGSKKGTNMNDLLKQAQKAQEEMDNLEDTFK
                     NIEVSASAGGGAINVVATCDYRIKSIEVDQELKEEDFEIVQDLIIAGVNEALSEVTKK
                     RDEETAKVTGALNLPDNIL"
     gene            complement(11122..12603)
                     /gene="dnaX"
                     /locus_tag="PLD15_03195"
     CDS             complement(11122..12603)
                     /gene="dnaX"
                     /locus_tag="PLD15_03195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPB62936.1"
                     /translation="MSEVLYRKYRPKNFNELVGQDQVKEILNKSFEKGSVSHAYIFSG
                     SRGTGKTTSARIFAKMLNCLSESSEKPCGICQSCLAIDTSSHLDVIELDAASYRGIDE
                     IRKIRDSAAYRPIMGRYKIYIIDEFHMLTREAFNALLKTLEEPPERVVFVLATTNIEK
                     VPETILSRCQIFTFKTLSEEEIVLYLERIATSESFSFSKDALTRIARAARGGMRDAVN
                     LMERVVAFTGSLDDQSVRLTLGILPDEVISSFLAAFSGVDPNRLLKVSKDILDEGLSY
                     ESLLEQSIDFLKEGYVKNMMRGNLQMISSLWEILKEMKYAEDKKAVFEVMTILKSFEL
                     TGLKEITGEILEKSDHEGTAVAKKDTQVKVEVLPRSTGGENVSRGPAEILLDHLYEKD
                     FTLLWVLLGLSRITLSEDGESLSVDSDDGFVAKLIEEKLDMINGISTEIVGKPVSISR
                     NKSPKALESLDKDQRAYVKNFMDVLGLSGNGALGEFKIEIEED"
     gene            complement(12742..>13404)
                     /locus_tag="PLD15_03200"
     CDS             complement(12742..>13404)
                     /locus_tag="PLD15_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB62937.1"
                     /translation="VEGVSSFCTSGTPADCVVAAFTLFGKNGFDIVVSGINHGQNLGV
                     DIRYSGTISAALEARTLGIPAIAVSVVSEENPDFSAALDFVDKFILYYDWRKLPRYTV
                     LNVNVPSVPRQNIYGIARTRPGGLVKRRWFEKNTNEWGEPVYWMRREIIYESEESDLD
                     FRSVTAGFISVSPIDFFQGCDEEYNEDLESDLRSFGDIWLDRNDPNTREFSNGRRLKD
                     TV"
BASE COUNT         2843 a         3662 c         3058 g         3841 t
ORIGIN      
        1 cccaggtggg tcaacaaaaa agagtgagag tgagagtgag agcgaaggcc tcactattac
       61 aggtgtcaat gcaaagcacg ttcgagaggg ccctcaccgc agctcttcga gtgcgctgtc
      121 tatcaaatcc gtggctaggg aagcgtccac cgtcgagatg agtcctatgg ctgcgaatat
      181 gtagttcctg tctctatata ttcttacatc cggtcccgga agcagctcct ttgaatggtt
      241 tccggacaac gatttcgcgt tatcaattat ctgtcgggtg tatctcgatc tgctcagaag
      301 cccccccgtt ccgactatgg attttatggc cgttagatcc tttccctggg cgacctctat
      361 cctcccggta ggaccgtaca tctgtcgtat atatcccgca tgacgcctta tggaagtctc
      421 gaagcagtat ctgccgagac gcgccgaaaa cctggaggct tcgtcgttaa ccgggtaagg
      481 agaaagcgag ccgacgatct cgtcaaagta agcgaactcc cctctcaact ccttctcacc
      541 gatgtgatca ataacgtggc gggcattgac atagacgccg aggtctccct cgaccgttcg
      601 cttcgagcgc ggctcgggcg agacgagcat tttcgagatc tccgggtcgc cgtcggtaac
      661 ggaatcgaca tcggtggtgg ccccaccgat atccacgacc agaacgtcgt tgtacacttt
      721 tgaaaaaagc tcggccgcga gcatcacagc ggccggggtg gggataaccg gtttgtttgc
      781 tatcgagtag atcttttcca tccccggtcc ctttatgatg tttctggaga agacctccct
      841 tatgattttc ctggtaggct cgacgttaag ttcgtcgatt ctgggataca cgttatcgac
      901 aaagtgcatc tctcttccag ttggagagat tatttcacgt acctcatcgg agacggcagt
      961 gttgccggcg taaacaagag gtatcgaccc gtcaagttga tttatcatac cggcgttgtg
     1021 gatcaccgtc tcttcttcgc cgtagtcgac gcccccggcc agcagaacga gtcgcggctg
     1081 aatccttgag attttctcaa gatcccgttt agaaagcttt cccgccgtca ccagctttat
     1141 cactccgccg gcccccagag ccgcctcttt cgctgcccta acggtcatat cgtaaacgag
     1201 gccgtgaaca gtcattctaa gaccgccggc cgcgctggag gaggcaatga aaacctccca
     1261 gtcgagccta tcatcattaa gtcgtttttt gagttcgatc agagcccttt ctattcctac
     1321 cgtcacatcg ttctcggcga cggtcgtgta cgattctccc tgggccagga aagacagctt
     1381 tccggatgcg tcgcacgaaa aggccgaaag cactgtcgta gtgctgccga tttcggctgt
     1441 gagtatctgg accttcatct gctctcgaga agctccctga ctattttgtt ggtcagtttc
     1501 ccatcggccc ttcccttgag tctgaccatc acctcgcgca taacctttcc aagatcggaa
     1561 ggtccgttgg ctttcagatc ttcgatagtc ttcagcgcga ctgcacgtat ctcgtcctcg
     1621 cccatttctt cgggaagata gtccttgagt atctccattt ccagggtttc ctgggccacg
     1681 agttcgtctc ttccggcttt gatgtaggcc tctatggatt cctctctccg tttcacttct
     1741 tttccgatca ggttgataac cgtatcatcc gtcacttcgg cctttctgtt ctcttcggaa
     1801 gaggcctgga agttttttac ggcagctatg acagatctca acgtgttggt cctgagcgtg
     1861 tcccgcaatt tcattgcttc tttcatatcg ctctgtatct tttcgtatag actcatagag
     1921 ctcactcctg tagttcctcc tcgctctgta gctcctcttc ttcaaaagag atcggcctta
     1981 cgagagggaa gggaattatg tcccttattg tgggcgaatc ggttaccagc attaggatcc
     2041 tgtcccagcc gatacccagc ccgccggtgg gaggcattcc atattccagc gccctcagga
     2101 aatcgtcgtc catcatctgg gcttcttcgt ctcccgcttc tctcagccca gcctgatgta
     2161 aaaaccttct gtgctgctct ataggatcgt tgagttcact gaaggcattg gccatctcca
     2221 tagaacttat tatcagttcg aacctttcag tcactctggg gtcgcttctg tggaccttgg
     2281 aaagtggtga gattatcacc gggtgctcgg tgacgaaggt tggattaacg agtttgtctt
     2341 cgacgagatc ccagagcttc tctatcaggt ggcccctctc tttcatgtcg ggctcgctgt
     2401 tgtgtttttt caggacctct atcagtcttt catctgaatc ctcgagtatg ttcgctccga
     2461 ggttgtcctg gatgaaatcg gccatcttca ccctgcgcca gggtctggaa aagtcgatct
     2521 ctcttccctg atagcttatc ctggttgagc cggccagatt ctcgacaatg tggttgatca
     2581 tcgcttcggt gatatccata atatcgttgt aatctgcata ggcccagtag agctccatca
     2641 tggtgaactc cggatggtgc ttgtaggata tcccctcgtt cctgaaattc ttcccgattt
     2701 cgaatatcct ctcgaacccc cctaccaggt atctcttaag aaagagctcc tctgcgatcc
     2761 ttagaaacat ttgcgattcg aagacgttta tccttgtttc gaagggcctt gccgaggctc
     2821 caccggtcag atggagaagc attggggtgt ccacttcgat gaaccttctc tgcgccagaa
     2881 actcccttat gagccggaaa agctcggagc ggatcttgaa gcggttgagc gcttcatcgc
     2941 tggagagcat ttcagcgtat ctctggcggt atattatctc tttgtccttc aggccgtgcc
     3001 acttttcggg cattgttctt atggacttag aaaggatctc gaagcttttg gcaaagaccg
     3061 acacctcgcc ggtatgtgtc ttaaagggga agcccgtgac accaacgaaa tcgcctatgt
     3121 tgacgtactt tttgaagatt tcgaagctgt cttttccgac cgagtcgagt ctgatatagg
     3181 cctgaatcct tccggtatcg tcgcgaagga caaaaaagga ggactttcca tgactcctca
     3241 aggccattac cctgccggcc gtccttatct ccacgtcttc ctttgtctga gaattctcca
     3301 gagtggagaa ctcgttcttt atgtccccgg cccagtgggt cttttcaaaa cgataagggt
     3361 agggttctac accttcctcg cgcatctgat ctatttcccg gagcctctgt ctccgtgtct
     3421 cttcactcat gactgcctcc gatcatttgc cgatggtgag tattttgtac ttggcccatc
     3481 cagacggggt tttaactctt acgacttcat cgactttgtg agtgagtatc cctttgccta
     3541 tgggggaatc ggaacttatt ttgccttcga atatgttggc ttcctgagcg tttacgattc
     3601 tgtactcaga tttctcaccg ttttccatgt tctgaagctc cactatgtga cccagcttga
     3661 ctgtgctcat atcgccatcg tcttctatga cttcggcgtt gttcagtatc tcctcgagtt
     3721 gttttattct gctgtcgatt cgaccctgtt cgtttttcgc ttcatcgtat tcgctgtttt
     3781 cactcaagtc gccgagctcc cgggcttcct ttattctttc ggcaatatcg tacatgagtc
     3841 tctttctgag gttttctagc tcctctttca ttctgtcgta gccttcctgg gtgaggtaaa
     3901 tggctttttt cttcacgacc cggatcccct ccattcaacg aacttaatcg ttctttagct
     3961 cttttattat atccatgaaa tgatctatgt ccctgaattc tttgtaaaca gatgcaaagc
     4021 gaacatacgc aaccctgtcc aggagcttga gtttagccat tatcatctct ccgatctcct
     4081 gtgagagaac ctcgtggcgc cccgacctgg tgacctcgtc ttcgattgaa tctactatcg
     4141 agctgagcct ttcgagcgaa attggtctct tctcgcatgc cctaagaaga ccgttcataa
     4201 tcttctctct gttgaatctc tctctcctgc cgtctttctt gattacaaag aaggggagtc
     4261 tttcgtacct ctcgtaggtg gtgaatcttc cgccgcagtc ttcgcattca cggcgcctcc
     4321 tgatcgaggt gttatcctcg gtcggcctgg agtcgagaac cctggtcgat tcgctggagc
     4381 agaatggaca tttcatcaga tctcccccat ctcttcggct atcgatatca gctttttcaa
     4441 cctattatat actgcggatt tactcatagg agggttcatc atctccccta gctctctcag
     4501 attaaggtct tcgttctcca gtctcaggat ggctacctgc ttcaattcgc tatcgagtct
     4561 ggcgagaccg atcttcgcgt cgatgattct gatggccctg acgtgtctgg ccatagcctg
     4621 tcccgatttg tttgcgttcg ccgtcagaaa attcagcgtg cggctcacgt tgccccttat
     4681 cttccttacc tctacagctc tggaaaggtg cgccaccgtg cgtttccctc ccataagaga
     4741 aagcaactcc atcaagtcgc cggccgattt gacgtagacc ttgaacttgt ttctgacttt
     4801 gacgactccg cacttgatgt tgaagttgtc atgaaggtaa tctctcaaag aatcgacgaa
     4861 ctctcctgta gtatcgagcg ttatttctag atggtattct ttagagggat ccactataga
     4921 accgcctgcc aggtacaggc ctctgacaaa ggctccaaaa taaacgggat cgtccctcac
     4981 cggcgagggt attatgtcgt ttctcactga aatgccgttg ctttcaagga atttttcgac
     5041 atctgcgaag atgtaactaa gctctccgcc gcgttttctt gaaaagcgga tttcttcggt
     5101 gacctgtgtt tcgtatatag gaagagagag tctcctggtg atctgaaaca atcttttgag
     5161 actggtgata gatgtcagaa gaatgaccag gtaagacgtt ccatctctta tcctgagagt
     5221 tcctctggac ttgacgtaac ccagaaactc cgccctgctc tcggaagtat cttcggtgga
     5281 tagatgacag agttcttcct tcaatttatc ggcgtaactc aaagcagaat ctcctcaaag
     5341 atctccagct ctctgagaat ggatacaacg ctctcgctgg agtgcctcac caccagcttc
     5401 tctctcccgt cggtgataag ctcggtagta gttccgcata tactggttac cctcgtatcg
     5461 tccagaagat cgttcattac cggtgccgcg ccggcccgcc tgtacctctc cagcacagcc
     5521 ttttcgacac cgccaacttc cgtccagaac acccggtcga atggagttcc ggtgtacgac
     5581 tctacggtat ctacgtgctt ttttagatcg aaaccctccg attcgttcgg ctgcgtcatt
     5641 atgttacaga tgtaaaccct ctttccggga gatctagaaa aggcttcact tactccggag
     5701 acgagaaaat tcggaacaac gctcgtaaat aggctgcccg gtccgacgat caacgtctcg
     5761 gcggtctcta tcgctctcaa aacttccggc aaggcctgaa cctttctatc gagagtgagt
     5821 cttttgatct tctttccgta cctcgcgacc gccgattcac cctcgacttt cgatccgtcc
     5881 tcgagctctc ctatcagacg aaccagtgcg tcggccaccg gtagaaccct gcccttaatg
     5941 ttcagcagtt tcgatgcggc gatgacggca tccggaaaac tgccatacat ctccgtaagg
     6001 cctgcgatta tgatgttgcc aagcgagtgt tttttcatag cgtctgtgga aaacctgtgt
     6061 gaaaacacct tcgtgagaat ctcttcatcg tttgccagtg cgatgatgtt gtttcttata
     6121 tcaccagggg gcggtatttt catttcttcg cgaaggattc ccgagcttcc tccgtcgtcg
     6181 gttatcgcga caacgagtgt gagctctacc gggaaaccct tcaacactct tgcaaatgta
     6241 gagagtccgg taccgcctcc gataagcact atctttctca agccaagcac taccttttga
     6301 ctgttattct ttcgttgact agatcgaccg gtacaacatc gccctctctc tggaagtatc
     6361 tcttgaggac tctctggaaa gattcggggt tgccgctcac gtagtaggtg acccgcgact
     6421 cttcaccatc tatctctccg atccagttta ttaaatccac cagtatgaaa tcggccgggt
     6481 ctatcagaag cgttttgcga gatatcgaag actctattac ttttcttatg aaaggatagt
     6541 gggtacagcc cagtatcaga taatccggct ccattcggtt gatttcggcc agataataac
     6601 tgaccacggc atcaactatt tcaccttccc agatgccttc ctcgattatt ggcacgaaga
     6661 gcggacaggc tttctgggtt attcgacgca gattttcgta tctgaaaagc ttcctcaaat
     6721 acagcgatct cctcactgtg ttgcttgtgg ctattaccga aacctccgat cctttcggag
     6781 cgatcgaacc taacgttctg acggtcggat ctatgatgct gaagtacggt acgggaagat
     6841 tctgcttttc cctctgagag agtacagagt ccgatgtgtt gcaggcagta acgatcgcat
     6901 cgatacccag ttgaaagaag aaatcgaaga tctccccgat atagccgcga agggtttcga
     6961 tgggtttcga cccgtaaggt acacgtgcag tatcggcgaa gtagaaaaaa tccacgccgc
     7021 ggggaaagcc ctgcaggagt tttttaagta cggtcagacc accaatgccg gaatcgaaga
     7081 ctccgatctt caaattatcc ttcaacacct tcacgtccct ggaagtattc cgaaagtacc
     7141 tcttcgtact tggctttcaa ccttaccagt tcgttctggc ttttaatgag atcatgggct
     7201 aggttcacac agatgcccac catcaacttt tcgaacccga tttcctcttc cttctttttg
     7261 aacgcgtcgt acatttcggt gacttttgaa aagacctgat cgacgacttc ctgaggatcg
     7321 ccgcttagaa aggagaactg gcgtcctccc aggttcaaag agactgatcg tttcatgatt
     7381 attactcctt atgttttttt gctgttttca tcgagactga ctttcactcg cttgataagg
     7441 gaagatatag tcttcgattc gctttcaagt ctgagctcga gctcgcgtat cttaagcccg
     7501 gcttcttcaa gcttttgttg cgactgttga acctgtttcc agagctgctc attctctttt
     7561 ttaaggcggc tgaattcttc gaccatcttg tcgacaacca gttcaagctc ctgataacta
     7621 tccaagaacc acaccccctc atcaggagat tatatcattt gagccggtaa agaaaactaa
     7681 atagccgccc ttcggggcgg ctatgaattc gaaatacctt attcgtggtt cttggctatg
     7741 gcaaccagct gtacgaagct ctcgagaaga ctcgctccac cgactaggcc gccatctata
     7801 tcttccatgg ccaccagaga gtcgaagtta tcgggcttta tgctgcctcc gtaaagtatc
     7861 ctgatgcttt ctgcgaggtc gtaatcgtag agcccgacca ggaattgacg gatgtatttc
     7921 atcgtctctt cagcctgttc cggcgttgcc accactcccg ttcctatggc ccagacaggt
     7981 tcataggcga ttataactcc ggaggcttcc tcttttgtaa aacccataag gccgttcttc
     8041 agctgtctct ccagaacgct gttggtgagg ttagcctttc tctcctcgag agtctctccg
     8101 atacagaaga ccggcgtcag gccgtttctc aggacggcct ttatcttctc gttggtctgt
     8161 gcatcggtct ccccgaagag ctgacgcctt tcagagtggc cgacgagaac gtaatcgatg
     8221 tacagttcct tcaacatcgg tacagaaatc tctccggtga aagcgccgcc atcggccgga
     8281 tggatgttct ggactccgac cttgatcttc gtgtctttca tcatgtctgc aacaccggaa
     8341 acggccagac tggtcgggaa gaccactacg tcaacagacg attcttctcc tatcgaagag
     8401 gccagaaagc cggcgaacat ccttgcctcc ccgggcgatt tgttcatctt ccagttaccg
     8461 gccaccatca atcttcgttt ttttttccgg tcgcattcgc cagactctct ataccgggca
     8521 tttcacgacc ttcaagcatc tccagcgaag cgccgccgcc ggtcgaaacg tgcgatactt
     8581 ccccggccag tccgaacttg tttatagccg cggcgctgtc tccaccgccg attatcgtaa
     8641 cggcgctttt tagagtggca agggctttgg caaccgtttc cgttccattc gcgaaatcgt
     8701 cgatctcgaa tacacccatg gggccgttcc agacgacggt tttggcatcc gcaagcttcg
     8761 atttgaagag ttcaacggtt ttcggtccta tatccagtcc cttccagccg gccggtattc
     8821 cctcatcggt catgacgact ttcttctcga ctccggcggc aatttccctt gccgttaccg
     8881 catctacagg aagcacgaaa tcgactttct ttcttgaggc cttgtcgagt atttctctgg
     8941 ccagttcgat tttgtcgtct tccacgagcg aatcgccgac gtttctaccc agcgccttga
     9001 gaaaggtgaa catcatggcg cctcctatga gtattctatc ggccttttcc agcaagttgg
     9061 ttatcacgcc tatcttgtcg gaaacttttg caccgcccag tatcaccacg taagggtgtt
     9121 cgggattctc tatggccatg ccgagcattt cgacttcctt ttccatcaaa aagcccgcta
     9181 tactgaccag atgagccgcc accccggtgt tggaagcgtg gcctctgtgg gccgttccga
     9241 aggcatcgtt gacatggatg tccgcaaaag cggccaactt cgcggcgaat tccgggttgt
     9301 tcttctcctc ttcgggatag aatctaacgt tttccagaag aagtatctcg ccgtctttca
     9361 tatcggaaac ggccttttcg acctccggac cgatgcagtc atcgacgaat ttgacaggtt
     9421 ttcctataag tttttcaagt ctggatgcga cgttcttgag ggtgtacttt ggatctttgc
     9481 cccctttggg tcttccaaga tgggagacca gaattgcctt cccgcccttt ccggttatat
     9541 attcaatcgt gggaatggcc gccgcgattc tcgtgtcgtc ggtcacttcc ccggtagtct
     9601 tgtccagcgg aacgttgaaa tcgaccctta caagaacttt tttacctttg agttcgacgt
     9661 ctttaagcgt taacttctta gccatacttt gaacctcctt ctcattaaag cggggccatt
     9721 aatggccccg ctccagaaag cttcggttac attttcatca ttttctctgc gagatcgaca
     9781 actctgcatg aatagccgta ctcgttgtca taccaggcgc acactttcac gagattgccc
     9841 atcgcagagg tgagcttgcc gtcgagaacg ccggaaaccg ttgttccgat gatgtccgag
     9901 gacaccagtt cttcttcgga gtactctata attcccttga gttcattttc agcggccttc
     9961 tttatcagag cgttcacttc ttcggccgtc gtttcctttt caacaatcac cgtcaggtcg
    10021 gttatggaac cgtctgttac ggggactctc atggctattc catcgagctt gcctttcagc
    10081 ccgggaatca ccagaccgac cgctttggcc gctccggtag aggtgggaat tgtgttggca
    10141 gccgccgctc tggcccttct caggtccttg tggggaagat cgagtattct ttgatcgttc
    10201 gtgtaagcgt gtaccgttgt gaggtatccc ttcacgatct tcagattgtc gttgagcacc
    10261 tttatgatcg gggctatcga gttggtcgta caagaagcat tggaaactac ctgcatctcc
    10321 ggcttcaact catcgtcgtt cactccgaga acgatcgtcg cgtcaaccgc tcctttggcc
    10381 ggggcggtaa tgagaacttt ttttgcgccg gcttcgacat ggggcatcgt cttttcctta
    10441 ctcgtgaaaa cgcccgtgga ctcaatgaca agctccactc ccagggcctt ccagggcaga
    10501 ttcgtcgggc tcttctccgc aaacaccttt atctcttttc catttacaac tatcgcccct
    10561 tcttttgcct gaaccgttcc cttaaacctt ccgtgaaccg agtcgtactt gagaagatgg
    10621 gccagggtcg ccgcgtcagt caagtcgttg atggcaacta tgtcgaatcc cccgcgcgcc
    10681 atcatttctc tgaaaacaag tcttccgatt cttccgaatc cgtttattgc tactttgtgc
    10741 atctgctaca cctcctaaag aatattgtcc ggcagattca gtgctccagt tacctttgcc
    10801 gtttcttcgt cgcgtttttt cgttacttcc gacagtgctt cgttcactcc ggcgattatg
    10861 agatcctgga cgatctcaaa atcctcttct tttaattctt gatcgacttc tattgatttt
    10921 atcctgtaat cgcaagttgc cacaacgttg atcgcacctc caccggccga agccgacact
    10981 tctatgttct tgaaggtgtc ctcgaggttg tccatttcct cttgagcctt ttgagcctgc
    11041 ttcaagagat cgttcatatt cgttcccttt ttcgaacctc cgtaatttct accgccaaga
    11101 cctctgagtt ttttggccat ttcagtcctc ctctatttca atcttgaatt ctccaagcgc
    11161 accgttaccg ctcaagccga gcacatccat gaagttcttg acgtaagccc gctggtcttt
    11221 atcgaggctt tcgagggctt ttgggctttt gtttctggag atggagacag gcttccccac
    11281 tatttccgtg gagatgccgt tgatcatatc gagcttctct tctataagtt ttgcaacaaa
    11341 gccatcgtcg ctatccacac tgagcgattc cccatcttcc gatagagtta tcctcgaaag
    11401 accgaggagt acccagagca aagtgaagtc tttttcgtag agatgatcca gaagtatttc
    11461 ggccgggccg cgcgagacat tctctcctcc tgtagaacgt ggcaggactt cgactttgac
    11521 ttgtgtgtct ttcttcgcaa cagccgtgcc ttcatgatca cttttttcca ggatctctcc
    11581 ggtgatttcc ttcagaccgg tcagttcaaa acttttcaat atggtcatta cttcgaagac
    11641 ggcctttttg tcttcggcgt acttcatctc cttgagtatt tcccagaggc tcgaaatcat
    11701 ctgcaggttg cctcgcatca tgttcttaac gtagccttcc ttcaggaagt ctatcgattg
    11761 ttcgagcaga gattcgtatg aaagcccctc gtcgagtatg tctttcgaga ctttcagcaa
    11821 tcgattcgga tcgacacctg aaaaggcggc caaaaaactg gagattacct catcgggaag
    11881 tattccaagc gtcaacctca ccgattggtc gtccagcgag ccagtgaagg ccacaaccct
    11941 ctccatgagg ttaaccgcgt ctctcatccc accacgagcg gcacgggcta tccttgtcag
    12001 agcgtctttc gaaaaggaga aggattcaga ggtcgcgatt ctttctaggt aaagtacgat
    12061 ctcttcttca ctcaaagtct tgaaagtgaa tatctggcac ctggatagga tggtctcggg
    12121 gaccttttcg atgttcgtcg tggccagaac gaagacgact ctctccggag gctcctcaag
    12181 cgttttcaac aaagcgttga aggcctcgcg ggtaagcatg tgaaattcat ctattatgta
    12241 gatcttgtag cgtcccataa tcggccgata ggcggcggag tcccttatct ttcggatttc
    12301 gtcgattccc cgatacgaag ccgcgtcaag ctcgatgaca tccaggtgag aagacgtatc
    12361 gatagctaga caggattgac atataccaca aggcttttca ctcgactcgg agaggcagtt
    12421 gagcatcttg gcaaagatcc gggcgctagt ggtttttccc gttcctctag aacccgagaa
    12481 gatataggcg tgcgagacag aacccttttc aaaagactta ttcaggatct ccttcacctg
    12541 atcctgacca accagctcat tgaagttttt cggcctgtac tttctgtata gaacctctga
    12601 catcttatca cccgttcaac tgaattatag cagattgcca taagtttgtt tgcccacaat
    12661 gaaaatattg aataaagata tgtgaaatag ttaacaatct gtactattcg agaacggttt
    12721 cccctgtcta taaatgtgtt cctataccgt atctttcagg cgtcgcccat tggaaaactc
    12781 tcgtgtattg ggatcgttac gatctagcca gatatcgccg aaggaacgca gatcgctttc
    12841 gagatcttcg ttgtattcct catcgcagcc ctggaagaaa tcgatggggc tgacggagat
    12901 gaagccggcc gtcacgctcc tgaaatctag atcggactct tccgattcgt agattatctc
    12961 tcttctcatc caataaaccg gctcgcccca ctcattggtg ttcttttcga accagcgcct
    13021 ctttacaagt ccacccggtc tggtacgcgc tattccgtaa atattctgcc gcggaacaga
    13081 gggtacgttt acgttgagaa cggtgtacct tggtaatttt ctccagtcgt aataaagtat
    13141 gaatttatcg acaaaatcca gagcggctga gaaatcgggg ttctcttcag acactaccga
    13201 aacggcgatc gccggaatgc caagggttct cgcttctagc gctgccgaga tggtacccga
    13261 atatcttatg tccactccca ggttttgccc gtggttgatc ccgctcacca ctatgtcgaa
    13321 accgttcttg ccgaagagag tgaaggcggc cactacgcag tctgccggcg tgccactcgt
    13381 gcagaaggaa ctgacacctt ccac
//
LOCUS       DAOOLY010000037        13375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5079_length_13375_cov_5.313589, whole genome shotgun sequence.
ACCESSION   DAOOLY010000037 DAOOLY010000000
VERSION     DAOOLY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5079_length_13375_cov_5.313589"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLD15_03205"
     CDS             <1..588
                     /locus_tag="PLD15_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB62938.1"
                     /translation="LLDSDDKYISLPSLKVFRKIKGYDSQLLGLAERLMNSEDEECRF
                     YGAQYLKGLQEPSSVEILHQGLEDPSDRVRALSLEALGNLLDHSIENVVEESLRSTSS
                     RLKKASLRAVKKLRLFNCGDLISRLLKNKKEEAAVRMLAASVTGFLKLEDLAGDLESA
                     IVDLSNDGRLRLAASRAMARINPSRLGELFGFTEG"
     gene            complement(575..1360)
                     /locus_tag="PLD15_03210"
     CDS             complement(575..1360)
                     /locus_tag="PLD15_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPB62939.1"
                     /translation="MITVPAGRYISTGSSIHAIDPSAKIVSFIALAFSSLFLDGPADF
                     LVYAVLWTAVFLLSAVALREYLSTLWGIRFLLLLVVVFQSLFTPGRVLLDLGVVRITV
                     EGLANGLIQSGRVIFAVLFGITLSLTTSPVEISTGFERILRRLGLGESRASKIGLAAS
                     LTLTFIPLISLQTERVIMAQKVRGVEFDKGSLFKKVKNALTVVIPVIVTSLKKAQDTA
                     AALQIHFRSSSRAGIHLRVSRWKLPEILLTATAFTALLGVILL"
     gene            complement(1357..1722)
                     /gene="queD"
                     /locus_tag="PLD15_03215"
     CDS             complement(1357..1722)
                     /gene="queD"
                     /locus_tag="PLD15_03215"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPB62940.1"
                     /translation="MFYVTKEFTFDAAHNLVSYHGKCEKLHGHTYKVHITMVGEPDGE
                     GMVVDFLELKAIARSKAIDLLDHSYINEIIPQPTAENIARWIFERVSPELKRENCRVY
                     EVTVWETPTSFAKYRVREI"
     gene            2174..2857
                     /locus_tag="PLD15_03220"
     CDS             2174..2857
                     /locus_tag="PLD15_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPB62941.1"
                     /translation="MFWDPTFIVLIPAIVLAAYAQFLVSSRFSQYSKVRSTFGYTGSQ
                     LARRLLDNAGLFDIHIERVKGNLTDHYDSRKRVVRLSQATHDSSSIAALGVVAHEIGH
                     AIQDREKYAPLVVRNAVVPVAQLGSSLSWILFVIGLLMVSPLLIKVGILVFSAFVFFT
                     LVTLPVEFNASSRAKKLLASMGMPSTELKAVGSVLGAAAMTYVASTATAILQLLRMLL
                     IAGAYGSRD"
     gene            2857..3867
                     /locus_tag="PLD15_03225"
     CDS             2857..3867
                     /locus_tag="PLD15_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPB62942.1"
                     /translation="MQTSRSIALETLSFFDSNGYISSKKVDMAISTLSFKDRAFCSNL
                     IFETLRKRIRIDYELSKYLRKPRGLPVSVLNALRMGALQLLFLDGVPPYAAIHSTVEL
                     IGVAEFRRLANAVLRKLSLNGLTREVPAHVEHSHPEWLFDLWRGIEYITDLEGLMEYN
                     QTPPSETYYSKGGERDMLENGYVFEKSEFSDLFVVFQKGGPTDGIERRDEMEFILERL
                     GLPIFKYPGSQTARLNSRPWLFHTLSRESLARSAEKIEASIVGRLKESPLEEFIYYSH
                     SLSLEENTSLVGGIPGYAARQMGTFLKDSGIEGRFDGHGYWLLPDSSPLPGYLCLLGR
                     IK"
     gene            3864..4907
                     /gene="rlmN"
                     /locus_tag="PLD15_03230"
     CDS             3864..4907
                     /gene="rlmN"
                     /locus_tag="PLD15_03230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPB62943.1"
                     /translation="MIDLLSTGLNQLRDLMGRLGEGSFRANQIFDWVYRKKVTDFDSM
                     TNLSKALRTKLREEMYFPRMEVVDKQVSSDGTEKFLWRLHDGEYIESVLLRHPEHCTF
                     CISSQVGCALGCSFCATGAGGFTRNLSPGEIVGQVLLMERELGSQVDNIVFMGMGEPF
                     LNEESLYASIDILHEPAGRNLGIRHFTVSTAGLPEGIVRLADSGRDLRLSVSLHSCDD
                     RTRSLLMPVNRRFSLSALRVSLDYYQRKTGNRLTFEYALVKGVNDSKEDVERLVKYLE
                     GLKAFVNVIPVNPVDSDHERPDEKTIDEFVKAIKSKGIEAALRYERGTDIAAACGQLR
                     RQKGGRAWKEEGE"
     gene            4886..5773
                     /gene="rsgA"
                     /locus_tag="PLD15_03235"
     CDS             4886..5773
                     /gene="rsgA"
                     /locus_tag="PLD15_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPB62944.1"
                     /translation="MERRRGIVVRFGSSNMEVVDQTSGGRVLCSMPGKFRAQGIRPIV
                     GDMVEYSLSSAGQGRIEAILPRKSELLRPRISNIEQIILVLSLREPEVQNFITDRFLV
                     MSSIAKIPVVVVVNKIDLLEGEELSKFTGIYGEYHEIIPVSSKKRINIEQMREVLKGK
                     ISAMAGMSGVGKSSLLNTLNPGLQLRVSELSRTLEKGRHTTSYAELLQFDFGGLIADT
                     PGFANLELPPLEVEDLQNHFPELAQERGMCAFSDCVHIDEPGCYVKELLKAGDISESR
                     YDSYFRMTRELKEIEGGRK"
     gene            5773..6420
                     /gene="rpe"
                     /locus_tag="PLD15_03240"
     CDS             5773..6420
                     /gene="rpe"
                     /locus_tag="PLD15_03240"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB62945.1"
                     /translation="MAKISPSILAADLSNLSREVRKVIGADLLHVDVMDGVFVPNITF
                     GTPIMEALGRIDHPPLDVHLMIEDPGKFIAEYVQLGARNLHVHVEGNPHLHRLITSIK
                     DLGASPFVVINPATPVQALEEILPFVDGVLVMTVNPGFTGQKFIPGASLKIDRLHRLR
                     DELGYSYEIAVDGGVSLSNAGELVERGADILIMGAAVFRAASPSKVVEKIRELKR"
     gene            6417..7001
                     /locus_tag="PLD15_03245"
     CDS             6417..7001
                     /locus_tag="PLD15_03245"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPB62946.1"
                     /translation="MRLYLVSGNRHKFQEIERLLPEGFDLDLITSIIPGKSVVEDAGT
                     FYGNALKKIEAYRNSGVYLLSDDSGLVIDALGGFPGVNSARFMEGASYRDKMLEILAR
                     MKGEKKRTARFVCAALFYDYSGGLLGVEGSVEGRIAPGISGTEGFGYDPIFIPDGYDR
                     TFGQLGYEVKNRLSHRAIAFKKLFSLVCLYNGQV"
     gene            complement(6955..7842)
                     /gene="lgt"
                     /locus_tag="PLD15_03250"
     CDS             complement(6955..7842)
                     /gene="lgt"
                     /locus_tag="PLD15_03250"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPB62947.1"
                     /translation="MKKKIVISSVLIAVAVAMFFFLRAVFSGELILNPIIVENLGPFT
                     IRWYGVLIAAAIITAYLLARSKAFKEGIKEEHIIEGIFLGIIFGVIGARLYYVAFNFE
                     YYRGDFWSIFRTWDGGLAIHGAFFAALLVAFLYVRFRKKAQLNFFQVTDLFTAVLPLA
                     QAIGRWGNFMNYEAYGGPTDLPWKMFVPEQYRMPGYSKFEYFHPTFLYESLANVAIFA
                     FLYWYLEKRRNYGEVTALYMILYSVVRFFVEGLRLDSLYIGQTELRTAKVVSVVLLVA
                     GVVLFILARYKGKPVKRVS"
     gene            complement(7922..9205)
                     /locus_tag="PLD15_03255"
     CDS             complement(7922..9205)
                     /locus_tag="PLD15_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPB62948.1"
                     /translation="MEKNFLNKEKNIEKFIFYFTPEEVEKAEGEVVKYVNQHYSIPGF
                     RKGKVPVSIVRSFLDESFDEMVLENLSETVEKEIKEEKLYIPASIVSQKREGDVASIE
                     VELHREPELKIKDLGELELMIPKRDDVVVNYVSNRLEELRDEHAIIEPKDGPVEIGDM
                     AKIEYSIVKDGKKISDKKAQEVQITAEDDRPMVKNLIGHSRGDTIQFNRTFEGSDNEY
                     FYSIELREVFKKSLPEIDDEFARTVDPDLSNLDELKKKIEEEGVKSFAGWKKDFLRQQ
                     AMDKIDDLVEIEIAESTIGFFVHRAIENSKKDKSYDGYLKQAESVEKLVEEFRAGILD
                     EVKKNRFIEEMARIEGLKVEEDELVSYSEEMSSHWGISADRAREMVKSREDVREDLIS
                     TILRNKVLDIVVEKAKVVEIEPREESPEQPGGKEE"
     gene            complement(9298..9765)
                     /locus_tag="PLD15_03260"
     CDS             complement(9298..9765)
                     /locus_tag="PLD15_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB62949.1"
                     /translation="MWMIMSDSHDNVPKVSRAIDLAIERGVSVVFHCGDLVSPFTARE
                     LLRYKGTLHVVFGNNDGERIGLKNLLGSSIGTGPLSLQVEGRKVVMMHEPFGLNDTIM
                     ADYIFYGHTHRIDTNLQGDRLVLNPGESCGYLTGRSTVVLLDPANRSYEVVEI"
     gene            9886..10545
                     /locus_tag="PLD15_03265"
     CDS             9886..10545
                     /locus_tag="PLD15_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPB62950.1"
                     /translation="MSAVKLAIRFSTGGLLRFLSHQETATAIERLIRRSSIPVSFTQG
                     FHPRMKISYSPAVPTGVGSLANYVLLDCDDRVDGVLELLKAHSVFTLRALGAWYLPVD
                     IKIDDFLDSYRFSLSLPAERFEPSRFDPQLTVVKKTKNGQRSFRAGEVFQNLTVTTLK
                     TCHMVEYFQPTERPVPSEELLKIVSREGTASNEGVFVYVKEGYFKNRATTNILDEIGG
                     I"
     gene            10547..11305
                     /locus_tag="PLD15_03270"
     CDS             10547..11305
                     /locus_tag="PLD15_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB62951.1"
                     /translation="MSGHNKWANIKHRKGAQDAKRSKVFTKIIRELMVSAREGGSDPN
                     TNTALRTAIEKARTANMPKDTMEKAIKKGAGEMEGQSFSEALYEGYAPGGVALLIRCL
                     TDNKNRTAQEIRHTLSKYGGTMAESGAVAWMFERKGLITVSKEQISDLEEFQLLAIEA
                     GAEDIKDDIDPIEIVTSPDAASNVKTALEENGYTLSYELNYIPSNSLKVSGSEAGRLL
                     KLLDILEDNDDVQEVYDNSDIDESEMEALAEEIG"
     gene            11348..11818
                     /gene="lspA"
                     /locus_tag="PLD15_03275"
     CDS             11348..11818
                     /gene="lspA"
                     /locus_tag="PLD15_03275"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPB62952.1"
                     /translation="MLSIFGMSLALIIDQVTKYLVEKNMTYFQRIDLIGEIFGLRYVK
                     NTGVAFGLFSSQEPLVLVSVAVSLVIAIVVLSVVFASKLSRFETFLVGVIVGGAIGNN
                     LIDRLRLGHVVDFLELRGFPAIFNAADSCIVVGAILLLVSMYRRERRDSRNTGD"
     gene            11793..12719
                     /locus_tag="PLD15_03280"
     CDS             11793..12719
                     /locus_tag="PLD15_03280"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB62953.1"
                     /translation="MIHEILVTNRENGWRLDKLVSEKAPPWISRTAIQRQIKDCKVFV
                     DRVSKKPGYRVKSGESITFELPDRPEAMSIEAEDLPLKIIYEDRDIVVVNKDPGVIVH
                     PLPRRQSGTLVNALLNHCVDLQGIGGVMRPGIVHRLDKDTSGVIVVAKNDLSHIALSA
                     QFKNRETSKDYLAIVRGKTPPTGKVAFSISRHPVNRLKMRVSEDGKESLTYFKTLATF
                     GGEASLVMTSPRTGRTHQIRLHMKEIGHPLLGDAMYGRAREDTIYGVERQMLHAARLT
                     LFHPRSSKKMTFIAPLPEDMKKVLVNLSVMAR"
     gene            12716..>13375
                     /locus_tag="PLD15_03285"
     CDS             12716..>13375
                     /locus_tag="PLD15_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB62954.1"
                     /translation="MKTAFIVTPYDLYQSVLLPEETARELERNGYERCVVIDRTLASF
                     VKWSKVLARHSITALPGIREGESYLVARNGEGFRELIAGSKSESPNLIRFEGRPKNLD
                     PSSPNPIWETRYLSRDRKKFEVYSSLKGVDLDGDYSLPDEERYLTLRNGIERESNLPE
                     SFSLKPYTQTFPERILPEDFRALLSGRLSDRGGEEEYLSRMDREVSVICLKGIQDYFM
                     TV"
BASE COUNT         3092 a         3558 c         3691 g         3034 t
ORIGIN      
        1 ctgctcgatt ccgacgacaa gtacatctcc ctgccctcgc tgaaggtttt tcgcaagata
       61 aagggctacg actcgcagct cctgggactg gccgagaggc tgatgaactc cgaggacgaa
      121 gagtgcaggt tctacggggc ccagtatttg aagggtttac aggagccctc ttcggttgag
      181 atccttcatc aggggctgga agatccttcc gatagggtca gggccctctc tctggaggcg
      241 ctgggcaatc ttctggatca ctctatcgag aacgtggtgg aggagtcgct gagatcgact
      301 tcgtcccgcc tgaagaaggc ctcgctcagg gccgtcaaga aactcaggct cttcaactgc
      361 ggcgacctga tctccagact cttgaaaaac aagaaggaag aggcggccgt caggatgctg
      421 gcagcctccg tcaccggctt cctcaagctc gaggatctgg ccggcgatct cgaatcggcg
      481 atcgtcgatc tctccaacga cggcaggttg agacttgccg cctccagggc catggcgagg
      541 ataaatccgt cgaggctggg agagcttttc ggtttcacag aaggataacg cccagcaggg
      601 ccgtgaaggc cgtggctgtc agaaggattt cgggcagttt ccatctcgat actcgcaagt
      661 gtatccctgc gcgactggac gatcggaaat gtatttgaag ggccgccgcc gtatcctgcg
      721 cctttttcag cgaggtgact atcacgggta tcactaccgt gagcgcgttc ttcaccttct
      781 tgaagaggct ccccttgtcg aactcgacac cccggacctt ctgggccatt atgacccttt
      841 ccgtctgcag ggatatcaga ggaatgaaag taagcgttag agaggcggcc aggccgattt
      901 tagaggccct ggactcaccg agccccagtc ttctgagtat cctctcgaaa cctgtcgaga
      961 tctcgacggg cgaggtggtg agtgaaagcg ttataccgaa aagcaccgcg aagatcactc
     1021 ttccggactg tatcagaccg ttcgccagcc cttccacggt aatcctgacc accccgaggt
     1081 cgagcaacac cctcccggga gtgaagagcg attgaaagac caccaccagc aggagaagaa
     1141 acctgatgcc ccagagggtc gacaggtact ccctgagggc cacggccgac agaagaaaga
     1201 cggccgtcca gaggactgcg tagacgagaa aatcggccgg tccgtcgaga aagagcgagg
     1261 aaaaggccag cgcgatgaag gaaacgatct tggccgaagg gtcgatggcg tggatcgaag
     1321 aaccggtcga gatataccgg ccggccggaa ccgttatcaa atctctctca ccctgtattt
     1381 ggcgaagctc gtcggagtct cccagacggt cacttcatat acccggcagt tctccctttt
     1441 cagttcgggc gatactctct cgaagatcca tctggcgata ttctcggccg tgggctgggg
     1501 tatgatctcg tttatgtacg agtgatcgag cagatcgatg gccttcgagc gggcgatggc
     1561 cttgagctcc aggaaatcta cgaccattcc ctcgccgtcg ggctctccga ccattgtgat
     1621 atgcacttta tatgtatgcc cgtgtagctt ttcacacttg ccgtggtagc tcacgaggtt
     1681 gtgagccgcg tcgaaagtga actccttggt tacgtaaaac atttttcatg cctccttttg
     1741 gtctattgga agagcggaaa gagccgtcct tggagaacct gaggctagag gtaagaggcg
     1801 agaggctgga agagccgtcc ttcgaaagat cgggagagcg gttcatcgtt ccgacgccgg
     1861 agggagagac tgaagagatg agatcccgta tgaagcacta cgggatgaca gttttactcc
     1921 cttcaggtca tcatggcaat ctcttagcca ggatctctac ctttcctcct tccagtcatg
     1981 ccggaccccg atccggcatc tcgcccttac tcaccaccca caactcacaa cgcaccacca
     2041 acaatccacc acccaacagc cgttcttggc acagtagcat tcgcccgctc cgtccaagag
     2101 tatagcatcg aaacgataac ggcatttgac cgtcaaatac aacggatata taattttaga
     2161 tggaggtggt tggatgtttt gggatccgac gttcatagta ctgattccgg cgattgttct
     2221 ggcggcctac gcccagtttc tggtgagttc tcgattctct cagtactcga aagtcaggtc
     2281 gaccttcggc tataccggtt ctcagctggc caggagactg ctggacaacg cgggactttt
     2341 cgatatacat atagagagag tgaaggggaa cctcaccgat cactacgact ccagaaagag
     2401 agtggtgaga ctttcgcagg cgactcacga cagcagctcg atcgccgctc ttggcgtcgt
     2461 cgcacacgaa ataggccacg cgattcagga tagggagaag tacgcccccc tggtcgtgag
     2521 aaacgccgtc gtgccggtgg cccagctcgg atcgagcctc tcctggattc tctttgtcat
     2581 cggcctcttg atggtctctc ccctgctgat aaaggtgggg atactcgtct tctcggcctt
     2641 cgtgtttttc acgctcgtga cgctgccggt agaattcaac gccagttcaa gagccaaaaa
     2701 gcttctggct tccatgggca tgccctcgac ggagctgaag gccgtcggct cggtccttgg
     2761 agcggcggcc atgacttatg tagcctccac cgcgaccgcg attctccagc tgttgagaat
     2821 gcttctgatc gcaggggctt atggaagcag ggattgatgc agacatcacg ttcgatcgct
     2881 ctcgaaacgc tttccttttt cgacagcaac ggatacattt cttcaaaaaa agtggatatg
     2941 gccatctcca ctctttcttt caaggatcgc gccttctgtt cgaacctgat tttcgaaacg
     3001 ctgagaaagc gaatcaggat agactacgaa ctttcgaagt acctgaggaa gccccgcggg
     3061 ttgccggtgt cggtgttgaa cgccctcaga atgggggccc ttcagctcct ctttctcgat
     3121 ggcgtgccgc cttacgccgc cattcactcc acggtggagc ttataggcgt cgccgagttc
     3181 aggaggctcg ccaacgccgt tctgcggaag ctctctctga acggtcttac tcgagaggta
     3241 cccgctcacg tggaacactc ccatccggaa tggctcttcg atctctggag gggtatcgag
     3301 tacatcaccg atctcgaagg cctgatggag tacaaccaga cgcccccatc ggagacgtat
     3361 tattcgaagg gcggcgagag ggacatgctc gagaacggct acgtcttcga aaagagcgag
     3421 ttttccgatc tcttcgtggt ttttcaaaag ggcggaccga ccgatggaat cgaaaggcgg
     3481 gacgagatgg agttcatatt ggaaagactc ggcctcccga ttttcaagta cccgggcagc
     3541 cagacggccc ggctcaactc caggccctgg ctcttccata cactaagcag agaaagcctt
     3601 gccaggagcg cagaaaagat agaggcgtcg atcgtcggga ggctgaaaga atcgcctctc
     3661 gaggaattca tttattactc gcactcgctg agcctggaag agaacacctc gctggtcgga
     3721 gggatacccg gttacgccgc caggcagatg gggacgtttc tgaaagattc cgggatagag
     3781 ggacgtttcg acggccacgg ctactggctg ctacccgatt catcgccgct ccccggttat
     3841 ctctgccttc tcgggaggat caaatgatag acttgctttc gacaggactc aaccaattga
     3901 gggatttgat gggccggctg ggcgaggggt ccttcagggc caaccagatc ttcgactggg
     3961 tttacagaaa gaaggtcacc gacttcgatt cgatgacgaa cctttcgaag gcgctgcgca
     4021 cgaagctgcg ggaagagatg tatttcccgc gcatggaagt cgtcgacaaa caggtttcgt
     4081 ccgacggcac cgagaagttc ctctggcgcc ttcacgacgg cgagtacatc gagtcggtgc
     4141 tcttgagaca tcccgaacac tgcacttttt gcatctcctc gcaggtggga tgcgcgctgg
     4201 ggtgctcctt ctgcgccacg ggggccggcg gcttcaccag gaatctttcg cccggcgaga
     4261 tagtgggaca ggtccttctg atggagagag agctcggctc gcaggtagat aacatagtct
     4321 tcatggggat gggcgagccc tttctcaacg aggagagcct gtacgcctcg atcgatatcc
     4381 tgcacgaacc ggccggaagg aacctgggca taaggcactt cacggtatcc accgccggtc
     4441 ttccagaggg aattgtgagg ctggccgact cgggcaggga tctcaggctg tcggtttccc
     4501 tgcacagctg cgacgaccgg accagaagtc ttctgatgcc tgtgaacaga aggttctcgc
     4561 tctcggctct cagggtttct ctggattatt accagagaaa gaccggaaac cggttgactt
     4621 tcgagtacgc gctggtgaag ggagtgaacg actcgaaaga agacgtggag aggctcgtga
     4681 aatacctcga gggactgaag gccttcgtca acgtcattcc ggtgaatccg gtcgatagtg
     4741 accatgaaag gccggacgaa aaaacgatag acgagttcgt gaaagccata aaatcgaagg
     4801 gaatcgaggc ggccttgaga tacgaacgcg ggaccgacat cgccgccgcc tgcggtcagt
     4861 tgagacggca gaaaggggga agggcttgga aagaagaagg ggaatagtag tcaggttcgg
     4921 aagcagcaat atggaagtgg tcgatcaaac gtcgggcggc agggttctct gctcgatgcc
     4981 tggcaagttc agggcgcagg gaatcaggcc gatcgtggga gacatggtcg aatattcgct
     5041 ctcgagcgcg gggcagggca gaatagaggc gatacttccc agaaagagcg agttgttgag
     5101 accgagaatt tccaacatag agcagataat cctcgtgctc tctctaagag agccggaagt
     5161 tcaaaacttc ataaccgatc gtttcctggt catgtcgagc attgcgaaga ttccggtcgt
     5221 ggtggtcgtc aacaagatag acctccttga gggggaggag ctttcgaagt tcaccggcat
     5281 ttatggcgaa taccacgaga taattcccgt ctcctcgaag aagaggatca acatagagca
     5341 gatgcgcgag gtgttgaagg gcaagataag cgcgatggcc ggcatgtcgg gcgtgggcaa
     5401 gagcagcctt ttgaacactc tcaatcccgg cctgcagctg agagtctcgg aactgtcccg
     5461 gactctcgag aagggacggc acaccacctc ctacgccgag ctcctccagt tcgatttcgg
     5521 cggactgata gccgatacgc cgggctttgc caacctggaa ctgccgccac tggaagtgga
     5581 ggatttgcag aatcactttc ccgagctggc gcaggagagg ggaatgtgcg ccttttccga
     5641 ttgcgtacat atagacgagc cgggctgtta tgtcaaggag ctgcttaaag ccggggatat
     5701 atccgaaagc aggtacgaca gttactttag aatgaccaga gagctgaaag aaatcgaagg
     5761 aggaagaaaa taatggccaa aatatcgcct tcgattctgg ccgcggacct ttcgaacctt
     5821 tccagagagg tgagaaaggt tatcggcgcc gatctgctcc atgtggacgt tatggacggg
     5881 gtcttcgttc cgaacatcac cttcggcact ccgatcatgg aggcgctcgg caggatcgac
     5941 cacccgccgc tggacgtcca ccttatgatc gaagacccgg ggaagttcat cgccgagtac
     6001 gtacagctcg gcgcgagaaa cctgcacgtg catgtcgaag gtaaccccca tcttcacaga
     6061 ctcataactt ctataaagga tctgggggct tcgcccttcg tcgttatcaa tccggcgacc
     6121 cccgttcagg cgctcgagga gatactaccc ttcgtcgacg gggtgctggt catgaccgtc
     6181 aacccgggat tcaccggcca gaagttcata cccggggcct ccttgaagat agaccggctg
     6241 catcgtttga gagacgagct gggttactcc tacgaaatag ccgtcgacgg gggcgtgagc
     6301 ctctcgaatg ccggagaact cgtagagcgg ggcgccgaca tcctcatcat gggggccgcc
     6361 gtcttcaggg ccgcttcgcc ttcgaaggtg gtcgagaaga tcagggagtt gaagagatga
     6421 ggctgtacct tgtgagcgga aatcgccata aattccagga gatcgagcgg ctgctgccgg
     6481 agggcttcga tctggatctt ataacttcga ttattccggg aaagtccgtg gtggaagacg
     6541 ccggcacttt ctatggaaac gccctgaaga agatcgaagc gtacagaaat agcggtgtct
     6601 atctcctttc ggatgattcc ggcctcgtga tagacgccct gggcggtttc ccgggcgtca
     6661 attccgcgag attcatggaa ggggcctcct accgcgataa aatgctggag atactcgccc
     6721 gcatgaaagg agaaaaaaag agaaccgcga gattcgtctg cgcggctctc ttctacgatt
     6781 actccggcgg ccttctggga gtcgaaggca gcgtggaggg gaggatcgcc cccggcattt
     6841 ctggaacgga gggtttcggc tatgatccca tcttcattcc cgacgggtac gacaggacct
     6901 tcggccaact gggatacgaa gtgaaaaaca gactttcaca cagggccata gccttcaaga
     6961 aactcttttc actggtttgc ctttataacg ggcaagtatg aaaagcacga caccggcgac
     7021 cagcaggacc acggacacga cttttgccgt tctcagctcc gtctggccta tgtaaaggct
     7081 gtccagtctc agtccctcga cgaagaacct gaccaccgag taaagtatca tgtacagcgc
     7141 ggtgacctcg ccgtaattcc ttctcttttc gagataccag taaagaaaag cgaaaatggc
     7201 gacgtttgcc agactctcgt acagaaaggt cgggtggaag tactcgaact tcgaataacc
     7261 gggcattcta tactgctcgg gcacgaacat cttccagggc agatcggtcg gaccgccgta
     7321 ggcctcgtag ttcatgaagt tcccccacct gccgatggcc tgggccaggg gcaggacggc
     7381 cgtgaaaagg tccgtcacct gaaagaagtt gagctgcgcc ttctttctaa acctcacgta
     7441 taaaaaggct accagcagcg cggcgaagaa ggccccgtgg atcgccaggc ctccgtccca
     7501 ggttctgaat atactccaga aatcgcccct gtaatactcg aagttgaagg ccacgtaata
     7561 gagacgcgct ccgatgaccc cgaagattat cccgagaaag atcccttcaa ttatgtgttc
     7621 ctcttttatc ccttctttga aggccttgct cctggcgaga agataggcgg tgattatcgc
     7681 ggcggctatc aacaccccgt accatcttat cgtgaatggg ccgagattct caacaataat
     7741 cgggttcagt atcagctcgc cgctgaaaac cgccctgagg aaaaagaaca tggctactgc
     7801 aaccgctatc aggactgacg atatcacgat cttctttttc ataactccac ccccaaaaga
     7861 tggtttccat ccagggagag ggccggaggc ctcccccgcg aacggaatcg gtttatacgt
     7921 tttattcttc ttttcccccg ggctgttccg ggctctcttc cctgggttct atctctacga
     7981 ctttcgcctt ctcgaccacg atgtccagaa ccttgtttct cagtatcgtc gatatcaggt
     8041 cctctctgac atcctctctg gatttgacca tctcgcgggc tctatcggcc gatattcccc
     8101 agtgagagga catctcttcg gagtaggata ccagttcatc ctcttccact ttgagtccct
     8161 ctattctggc catctcttcg atgaatctgt tcttcttcac ttcatcgagt atgcccgccc
     8221 tgaattcttc cacgagtttt tccacgcttt cggcctgctt caggtacccg tcgtagctct
     8281 tgtccttttt cgagttctcg atcgccctat ggacgaaaaa gccgatcgtg gactcggcta
     8341 tctcgatttc caccagatca tctattttgt ccatcgcctg ctgacggagg aagtcctttt
     8401 tccagccggc gaaggatttc acgccctcct cctcgatctt cttcttcagt tcgtcgagat
     8461 tagatagatc gggatcgacc gttctggcaa actcgtcgtc gatctcgggc agactcttct
     8521 tgaagacttc ccttagctct atgctgtaaa aatactcgtt gtccgatcct tcgaaagtcc
     8581 tgttgaactg gatggtatcg cccctggaat gaccgatcag gtttttcacc atcggcctgt
     8641 cgtcctcggc ggtgatctga acctcctggg ccttcttgtc ggagatcttc ttcccgtcct
     8701 tcactatgga gtactcgatc ttcgccatgt ctccgatctc gacaggaccg tctttcggtt
     8761 cgattatcgc gtgttcgtcc cgcagctctt cgagcctgtt gctgacgtag ttgacgacca
     8821 cgtcgtctct ttttggtatc atcagttcga gctcgccgag gtcctttatc ttcaattccg
     8881 gctctctgtg gagttccact tctatcgaag ccacatctcc ttcgcgcttc tggctgacta
     8941 tggacgcggg aatatagagc ttctcctcct tgatctcttt ttctacggtt tcggaaaggt
     9001 tctcgagaac catttcatcg aaactctcgt cgaggaagct tctcacgatg cttacgggga
     9061 ccttgccctt cctgaatccc ggtatcgaat agtgctggtt cacgtacttt acaacttcac
     9121 cctcggcctt ttcgacttcc tcgggcgtga agtagaagat gaatttctca atgttcttct
     9181 ccttattcaa aaaattcttt tccacttatc tttccctcca ttcagactgt tatttgctgt
     9241 ccaattatac ttatccatac cgatggcacg gttaaactga tgttattatt gaccggttca
     9301 gatctccacc acctcgtacg atcgattggc aggatcgagc aggaccacgg tggatcttcc
     9361 cgtgaggtat ccacagctct cccccggatt caaaaccagt ctatcgcctt gcaggttcgt
     9421 atcgattctg tgagtgtggc cgtagaagat gtaatcggcc atgatggtgt cgttcaggcc
     9481 gaaaggttcg tgcatcatca ccacttttct gccttcgacc tgcagagaaa gagggccggt
     9541 tccgatcgaa gatcccagaa ggttcttcag gcctatcctt tcgccgtcgt tgtttccaaa
     9601 gacgacgtgg agcgtacctt tgtatctcag cagttccctc gcggtgaagg gagagaccag
     9661 atcgccacag tgaaaaacaa cggagactcc cctttcaatg gcgagatcga tcgctctgga
     9721 aaccttcggt acgttgtcgt gagagtcgga cattatcatc cacatttgta cacctctatt
     9781 caaaaaatca atatatcatt gcaaagtccc gcagtatcgc atacaattat aactgcacaa
     9841 ctgtcgaaca acaaagggtt tctgttcagt cacctggagg gtcttttgag cgctgtaaaa
     9901 cttgcgattc gtttctctac gggcggtctt ctgaggtttc tgtctcatca ggagacggcg
     9961 acggctatcg agaggttgat ccgcagatcg tcgatccccg tttccttcac ccagggcttt
    10021 catccgagaa tgaagatcag ctactcgcca gccgttccca ctggcgtggg cagtctggcc
    10081 aattacgtgt tgctggactg cgacgatagg gtggatggcg ttctggaact tctgaaagca
    10141 cattcggttt tcaccttgag agccctggga gcgtggtacc tgccggtgga tataaagatc
    10201 gacgattttc tggattccta caggttttcg ctgagtcttc ccgcagaacg cttcgagccg
    10261 tcccgcttcg acccgcagct gactgtggtg aaaaagacca agaacgggca gcgctctttc
    10321 agggccggag aagtttttca aaacctgacg gtaacaactc taaaaacctg tcatatggta
    10381 gaatatttcc agccaacgga aaggccggta ccatcggaag aattgctgaa gatcgtatcg
    10441 cgagagggaa ccgcttctaa cgaaggggta tttgtttatg taaaagaagg ttacttcaag
    10501 aatagagcta cgactaatat tctggatgaa ataggaggga tttgaaatgt ccggtcacaa
    10561 caaatgggcg aatatcaagc acagaaaagg tgctcaggat gccaagagat ccaaagtttt
    10621 caccaagata atcagagaat tgatggtttc tgccagggaa gggggctctg accccaacac
    10681 gaacacggcc ctcaggacgg ccattgaaaa ggccaggacg gccaacatgc cgaaagacac
    10741 gatggaaaag gccatcaaga agggcgccgg agagatggag ggacagtcgt tctcggaagc
    10801 gctctacgaa ggttacgccc ccggcggagt ggcgctcctg ataaggtgtc tgaccgacaa
    10861 caagaacagg acggcccagg agataagaca cacgctttcc aaatacggcg gcaccatggc
    10921 cgagagcggt gcggttgcct ggatgttcga aagaaagggc ctcatcacgg tctccaaaga
    10981 gcagatctcg gaccttgaag agttccagct tctcgcgata gaggccggcg cggaggatat
    11041 aaaggacgat atcgacccca tcgagatcgt cacctctccg gatgccgcct cgaacgtcaa
    11101 gaccgccctc gaagagaacg gctacactct ctcttacgag ttgaactata tccccagcaa
    11161 cagtctcaag gtatcgggct cggaggccgg caggcttttg aagcttctgg atattctcga
    11221 ggataacgac gacgttcagg aagtctacga caactccgat atagacgaat cggaaatgga
    11281 agcgctggct gaagagatcg gctgagattg atggccccgg gagggcgcac tatctggagt
    11341 ggttttattg ctttcaatat ttggtatgtc tctggcactg ataatcgatc aggtaaccaa
    11401 gtacttagta gagaagaaca tgacgtattt tcagaggata gatctgatcg gtgagatttt
    11461 cggtctcagg tatgtcaaga acacgggagt cgctttcgga cttttcagca gccaggagcc
    11521 gctggttctc gtttccgtcg cggtctcgct ggtcatagcg atagtcgtgt tatctgtggt
    11581 cttcgcttcg aaactgtccc ggttcgagac tttcctggtc ggtgtaatcg tcggcggggc
    11641 gatcggcaac aacctgatag acaggttgag gctcgggcat gtcgtggatt ttctcgagct
    11701 cagaggcttt ccggcgatct tcaacgctgc cgatagctgc atagtcgttg gagcgatcct
    11761 gctgctcgta tcgatgtatc gaagggaaag acgtgattca cgaaatactg gtgactaacc
    11821 gggagaacgg ttggcgcctc gataaactgg tttctgagaa agctcctcca tggatatctc
    11881 gtacggccat tcaacgccag ataaaagatt gcaaggtctt tgtcgacagg gtctcgaaga
    11941 aacccggcta cagggtcaaa tccggagaat cgataacctt tgagcttccc gacaggcccg
    12001 aggcgatgtc gatagaggcg gaagatttgc cgctgaaaat aatctacgaa gatagagata
    12061 tcgtagtggt gaacaaagat ccgggggtta tcgtacaccc gctgccgcgc aggcaatcgg
    12121 ggacgctggt gaacgctctt ttgaaccact gtgtggatct tcaggggatc ggaggcgtga
    12181 tgagaccggg aatagttcac aggctcgaca aggataccag cggtgtgata gtcgtcgcca
    12241 agaacgatct ttctcacatc gccctctcgg cgcagttcaa aaaccgcgaa acatcgaagg
    12301 actatctcgc gatagtgagg ggaaagactc cgccgacggg gaaggtggct ttctcgatct
    12361 cccgacaccc agtcaacagg ttgaaaatga gagtgagcga ggacgggaag gaatcgctga
    12421 cttacttcaa aacgctggcg accttcggcg gggaggcttc gctggttatg accagcccga
    12481 gaacggggag aacccaccag ataaggctgc atatgaagga gataggccac cctctcctgg
    12541 gtgacgccat gtacggcagg gcgagggagg atacgatata cggagtggag cgccagatgc
    12601 ttcacgccgc gaggttgacc ctgttccatc ccagatcttc gaaaaagatg actttcatag
    12661 cgcccttgcc cgaggacatg aaaaaggttc tggtgaacct gtcggtgatg gcgagatgaa
    12721 gacggccttc atagttaccc cttacgatct ctaccagtcc gttctgctcc cggaagagac
    12781 ggcacgcgag ctggagagaa acggctacga aagatgcgtc gtaatcgacc ggaccctggc
    12841 ctctttcgtg aagtggtcga aggtgctggc ccggcattcg ataacggccc tcccgggaat
    12901 cagagagggc gagtcttacc tggtcgccag aaacggggaa gggtttcgcg aacttatcgc
    12961 cggctctaaa agcgaatcgc ctaacctcat caggttcgaa ggccggccga agaatctcga
    13021 tccctcctcg cccaatccga tctgggagac gcgctacctg agccgggata gaaagaagtt
    13081 cgaggtctac tcgtccctca agggagtcga tcttgacggc gactactccc tgcccgatga
    13141 agagagatac ttgaccctcc ggaacggtat cgaaagagag tcgaaccttc ccgaaagctt
    13201 ttcgctcaag ccctacacgc agactttccc cgagaggatt ttgccggagg acttcagagc
    13261 gcttctctcc ggcaggcttt ccgacagggg cggcgaggag gagtatctat ctcgaatgga
    13321 tagagaagta tcggtcattt gcctcaaggg gatccaggat tacttcatga ccgtg
//
LOCUS       DAOOLY010000038        13289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5136_length_13289_cov_6.171150, whole genome shotgun sequence.
ACCESSION   DAOOLY010000038 DAOOLY010000000
VERSION     DAOOLY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13289
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5136_length_13289_cov_6.171150"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLD15_03290"
     CDS             complement(<1..425)
                     /locus_tag="PLD15_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB62955.1"
                     /translation="MKKRSLGKTWIYLLLIAGAVFFMIPFFWMFSTSLKSYDDVFAFP
                     PVWWPSEVLWSNYADLLVELPFLRYTLNTVIVTGMTLLGFMLSSSVVAFGFSYFRIPG
                     KKVLFRILLVTLMIPPQVTMIPQFVLFNKLGWVGTFLPL"
     gene            complement(422..1321)
                     /locus_tag="PLD15_03295"
     CDS             complement(422..1321)
                     /locus_tag="PLD15_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62956.1"
                     /translation="MSKVAKSRLREARIGLLFLSPWLIGLAVFTAYPIIASLYFSFTH
                     YSGLSSPRWAGLDNYVQLFKDPLFWKSIYNTLYLAIIGIPLAQVLSILVALLLNTKVK
                     FLGIFRTLYFLPSIVPAVATALLWKWFLNPQFGPVNLLLEKIGLPTPGWLTDPAWSKP
                     ALILTSVWGVGGAMVIYLAGLQNIPTELYEAAELDGAGKIARLFTITLPMLSPVILFN
                     VVMGIINSFQSFTSVFIMTGGGPENSTLVYALSIYRNGFQFYKMGYASAMAWVLLIMT
                     SIAIVFVFKFFGKYGYFGGVDKQ"
     gene            complement(1325..2578)
                     /locus_tag="PLD15_03300"
     CDS             complement(1325..2578)
                     /locus_tag="PLD15_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62957.1"
                     /translation="MKKVLAITVILAVAMVGLAKTELSFWYAWGGDEGKALLALVEEF
                     NASQNEIVVRPVFVPIGQGEKINTALAGSSTPDIVTIWDWMVVPLGESGALIPLNDYL
                     GSAGIDESDYLPGVWSYGAYRGIKYGLPTTLNAYAFMWNKALFKEVGLDPDRPPENIQ
                     ELDRYAELLFKQDTRGNIRRLGFLPNVSHIYFYVFGGQLWDPETEEITANHPGNVAAL
                     DWVASYYKKFDLQKIRVFQATWGEMASSYNPFYRGQLAIQEAGQWELLFIKQFAKSDF
                     EYGVSPFPAPEGGREKVAYLNGSFWAIPKGSKYPDQSFEFLKWLTDKDQAARFAAELS
                     NIPPRVDAIDNQAFQSKMLPNMEIYIDMLTNGYVYFFPTLPIGLYYMEELNQALQYVQ
                     SGAKTAEQALNDVQQAVIRELGRYE"
     gene            complement(2581..4014)
                     /locus_tag="PLD15_03305"
     CDS             complement(2581..4014)
                     /locus_tag="PLD15_03305"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HPB62958.1"
                     /translation="MKVIAIFLVLVVSGLTAFAGSGEVKNITGAFIQLDGQLSQFTEE
                     QWKSELSLMKDIGMDTVVIQYSAYGERFYYPSRYMKTDEISPDESIAELVWSGSTRTR
                     FVKIVIEPTSTEWTMIPEIAVKKGEEALSAGKTYTVDPQANPSYPSGGKLTDGVADYA
                     WGAMVGWQYPEKPIEIVVDLGELTTIDSIAVKFMRSEISGVQIPSKGYEVAVSNDGKA
                     FMSAGKVTWEENKQEKANDTLGELLKAADSLDMKVFLGLSLNPSYWSGEFDPKEQINM
                     NQKILTELFSLYGAHASLAGWYLPEEIDDRNFLTQTRKDGIRTYLKSMVTYAKFLTRK
                     PVMISPYFGINPNGEAYAEWWDYVLSEAKVDIIAMQDGVGTRRTSVAESAGVIKALKP
                     IMDKHGVRFWVNVEVFDQIHGWPVDAGSWQAVPADIERVVDQLQMQSPYTEKVVIFDF
                     PHYMTPRLGGKAEELYKAYKEYTGLGD"
     gene            complement(4279..5325)
                     /locus_tag="PLD15_03310"
     CDS             complement(4279..5325)
                     /locus_tag="PLD15_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB62959.1"
                     /translation="MSKIRMAIIGAGVWGETHAFLYREHPAVELVAICDLDLSRARSF
                     AQKFEIPHFFTDHIEMLESLDFDAAAIVTPDFAHGRIAVDCANAGKDLLVEKPLATTR
                     EDLAAIVGAVSKNRVRIMTDLHNRWSPPFAVAKEMLDGGELGTPVSAYFRLNDVVWVA
                     TDMLPWSARSSILWFLGSHSVDTLRWFFNDEVESVYSVSSGGVLKNMGLDTTDIYQTL
                     LKFRKGGIATMENSWITPNTNPCVNDIKFNFTGSRGMVNLDLSNNQMIESYTQREAKR
                     PDVLVRHFVHGKAKGFAYESIRHFIDCLASGEEFLVSLEDAVNTSLVILSIFESVRTG
                     EPEKVETIEALSAI"
     gene            complement(5313..6758)
                     /locus_tag="PLD15_03315"
     CDS             complement(5313..6758)
                     /locus_tag="PLD15_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HPB62960.1"
                     /translation="MKAVVLPMDERPPNYRIVQKIGKMLGLEVRLPGRELLGRYMRPG
                     RCEELGRWLLEESGDAFAISVDMLLFGGLIASRAGDVSEFEALKRLELLRELRERNPR
                     SRILLSSIVRRASISVSSAGSRELWERLNRYLRAIGNDEEDEARSIESTFPRGFLESY
                     YRLRSRNHIVNRACVELVKEKIADLLVLAQEDTFPDGPQKCELEILDKLSRESGVKES
                     VFIHNGADEVIQELLTFTSADRQPLSVDLTYDSPETEKKIMDFEDRQFGENVGSHMRL
                     VGLTVSGESETSIFIAGSEVERALEKLEALSKKKKRIFILDVFRANGSNRQFVEGFLN
                     MGLENIWGYSAWNTASNSLGTLLAVVVAACRSTNSCREIAAFYLSRLLDDHLYQGVLR
                     DELEKRIRNVGGDEYRVGDSGLLFEEFRDGLFIPRAVELMEKHFYGRRHDVFGGFVEK
                     GSISVTDFFLPWDRTFECDLELREEVRECRR"
     gene            complement(6755..8083)
                     /locus_tag="PLD15_03320"
     CDS             complement(6755..8083)
                     /locus_tag="PLD15_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB62961.1"
                     /translation="MDRVYDVIVVGGGIAGVSAAVAASRLGAGVLLIEKGMSFGGVLT
                     GSLVNPMMTFHSHRRRVIGGIGQEIVERLVRERGSHGHLEDPIGFVKSITPFDPEKMK
                     SVLIELLREARVDYLFNSLVGRVWVEKGFLQRVETVNANELRTFIGRAFIDATGEGNL
                     SIKAGARYVNGDGNRANCQPMTLVMRIGGINRDEIISFIGENESDFVLGEKRDFSYLA
                     VAGFFSLMPLLRNYRVNFRRDRLLFFEIPFHPGEVFMNTTRYAGFGGSAVELTAAQSD
                     GNIDVWKFMDFLKSEVPGFANASLIQTGCSIGVRETTHVTGEYMMKVDDLLGQKEFVD
                     KIAIGSYPVDLHVPESGELKTMKIPFPGEYSVPLRALFPLGLENVVLAGRALSAEHLA
                     FSALRTSPLASATGMAAGICAALSKGSHYRVREVPIKTIQKEIARMGGIL"
     gene            complement(8096..8953)
                     /locus_tag="PLD15_03325"
     CDS             complement(8096..8953)
                     /locus_tag="PLD15_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB62962.1"
                     /translation="MKKFKRLRVIFLLLIAAVVMGSPFFWMAISSLKPNAELFSWPPT
                     FVPRHLTLDHYTTALTTRGFERYFLNSAVVSLISMAFNLVFCSLAGYAFARLDFPLKN
                     FLFLLLLSTMMIPVQVTLIPTFLMVKSFPLAGGNNIFGQGGTGLLNTYAGLVVPHIMS
                     VFGVFIMRQFYMQFPRELSEAARIDGAREISIFTRIFLPLGKPAMSALAIFTFTQAWD
                     DFLWPLVVTSDRNMRTLQLGLEVFKNRYTADWGPLMAATTVSILPVILIFVVFQRYFT
                     DTALSSGIK"
     gene            complement(8950..9831)
                     /locus_tag="PLD15_03330"
     CDS             complement(8950..9831)
                     /locus_tag="PLD15_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62963.1"
                     /translation="MTNRLKKRIMLTAFIFISPVLVYNIIFRIAPIFVSLYLSFTKFS
                     GFGSAKLIGLVNYSRIFGDTQFWSAVLRTLQFSVEVLPLNMLLSLLLALLVNTGLKGM
                     SVFRAIYYLPVVTPMVAASMIWLWLYDPQIGILNFLLSLFNIPPQNFLRSPATAMHSI
                     VAMRVWRGAGWNMLIYLAGLQGISRNLYEAAAIDGASAFKRFLKITLPLLRPVHIYVL
                     IVGLISTLQSFTEMYVMTSGGPLESTTTVGLLIYRAAFDYMDMGYASAMSFILGLIIM
                     TLSVVSFTWRREKEVAE"
     gene            complement(9891..11123)
                     /locus_tag="PLD15_03335"
     CDS             complement(9891..11123)
                     /locus_tag="PLD15_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62964.1"
                     /translation="MKKLVLVLSVLSLLVFVGFGKTNVTVWFAGTSDQLTIAMDDDLI
                     PAFEKANPDLKLVVEYIPWGELSTKLTTAFAGGIGPDIFMHGQAAIAGFVENGVIRSI
                     DDLIARLDDPADFGAALDVGIYKGKNYFVPVFGSGRLLAYRADFFEQSGLDPNEPPVT
                     WEQLVTFAKATTIYDSGRIVRAGLDIPVTGIDLQQVWTSFLIENGGKLFDDELNPVFN
                     SPAGVEALEYYVGLVREAKVASEFGLTPVGNLEPIAAGTAVMVFMNNESLAALKEYSP
                     ESYSKIRLAFPPARYTRAGFYSFAGFMVSSSTKNLEATTKALAFLTSKDSLVKINKAL
                     GSLPPRQSGAQADFIANDPDLKKFVEGAKYAYGNPNVPFWVQARDIISKYLERAVMGA
                     MNPKDALDEAAKEVSKLK"
     gene            complement(11139..11816)
                     /locus_tag="PLD15_03340"
     CDS             complement(11139..11816)
                     /locus_tag="PLD15_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62965.1"
                     /translation="MIFSQLLKSERKLLIVSLPENTFEFARAALENGADAVKLHVNVK
                     HRVTGKLHATWDGIRETVGRIKSEFDPCMGIVPGAENMSGAGEIEEMEKSGISFFDVY
                     VDYAPLYLLRNGMSKMLALNYTYDPSMAGNLKSLGADCVEVSTVNPEKYGSPLTLMDL
                     LRYREVIERAGLPCFLPTQKKVMPEEVKELFEIGFRGLIIGPIVTGTDIKSFSSVVKS
                     FSEAIRY"
     gene            complement(11819..12817)
                     /locus_tag="PLD15_03345"
     CDS             complement(11819..12817)
                     /locus_tag="PLD15_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB62966.1"
                     /translation="MEVTLKEISTRAGVSISTVSRVLKNDPRISESTKEKVLRVARKL
                     GHPVEPGSISSNRVIMFFVSNPHRSIESDEFFSGVQRGILQTSSGEGLHCLVQSMRSR
                     SAFDESLIPVDLVEGLVVGGIPMPESMIHFLMSLKIPTVLVGKYCGLESFPSVNNDNV
                     RGGYLAAYELLKNNYEKITVITGPREVSTFADRLEGFFKCLKENNYPGENVRVIECNS
                     FEERDGKRFVEREYRKSERREAIFCTTDWLAKGVMEAFFEQEVAIPGQVGVIGFGGLD
                     FTRRIRPRLTTIALNPYLLGKIATVMLSELLEGNDEARGVVFVEPYLLKGETLKGG"
     gene            complement(13019..13195)
                     /locus_tag="PLD15_03350"
     CDS             complement(13019..13195)
                     /locus_tag="PLD15_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62967.1"
                     /translation="MGRTRRGERERREKERERKREREREIKREREREIKREKEGELST
                     FFLLCFTNNGRLFA"
BASE COUNT         3012 a         3715 c         3390 g         3172 t
ORIGIN      
        1 atgagcggca ggaacgtgcc gacccagcct agtttgttga agagaacgaa ctgcggtatc
       61 atggtgacct gcggcggtat catcagcgtg accagcagta tcctgaagag aaccttcttg
      121 cccggtattc tgaaatagga gaaaccgaag gccaccacgg aagacgaaag catgaagccc
      181 agcagagtca ttcccgtcac tattacggtg ttgagagtgt acctcaagaa gggcagctct
      241 acgagcaggt cggcgtaatt ggaccacaac acttcggaag gccaccatac aggcgggaag
      301 gcgaaaacgt cgtcgtagga tttgagagag gtcgagaaca tccagaagaa cggtatcatg
      361 aaaaacacgg cgccggctat cagcaagagg tatatccatg ttttaccgag cgatcttttc
      421 ttcattgctt atctactccc ccgaagtaac cgtattttcc gaaaaacttg aagacgaaga
      481 cgatcgcgat ggaagtcatt atgagcagca cccaggccat ggcgctggcg taacccattt
      541 tgtagaactg gaagccgttc ctgtagatcg acagcgcgta gaccagcgtg gaattttccg
      601 gtccgccacc ggtcattatg aagacactgg tgaaggactg gaaggagttt attataccca
      661 ttaccacgtt gaagagtatc accggactga gcattggaag agttatcgtg aaaagcctgg
      721 cgattttccc ggcgccgtcc agttcggccg cttcgtagag ctcggtgggt atgttctgca
      781 aaccggccag atatataacc atcgccccgc cgactcccca gacactcgtc aagatcaggg
      841 ccggtttcga ccaggccggg tcggtcaacc agcccggcgt gggaagacct atcttttcca
      901 gcagcaggtt caccggtccg aactgcggat tgaggaacca cttccagagg agcgccgtcg
      961 caacggccgg aacgatactc ggcaaaaagt acagggtcct gaatatcccc aggaacttga
     1021 cctttgtgtt caacagtagg gccacgagga tgctcaggac ctgtgcgagg gggataccga
     1081 ttatcgccag atacaacgtg ttgtagatag acttccagaa cagaggatct ttgaagagct
     1141 ggacgtaatt gtcaagtccg gcccagcgtg gcgaagatag cccgctgtaa tgcgtgaagc
     1201 tgaaatacag cgatgcgatt attggatagg ccgtgaagac ggcaagaccg atcagccagg
     1261 gagaaaggaa gagcaaaccg atacgcgcct ctctaagcct ggacttcgcc actttgctca
     1321 tgggttattc gtacctcccc agttccctta ttacggcctg ctgaacatcg ttcaaagcct
     1381 gctcggccgt cttggctccg ctctgtacgt attgcaacgc ctgattcagc tcctccatgt
     1441 aatacagtcc gatcggaagc gtggggaaga aatacacata accgttggtc agcatatcta
     1501 tgtatatctc catattcgga agcattttcg actggaaggc ctgattgtct atcgcatcga
     1561 ccctgggggg gatgttcgac aactcggcgg cgaaccgggc ggcctgatct ttatcggtca
     1621 accacttcag aaactcgaag gattgatcgg ggtacttcga acccttgggg atagcccaga
     1681 aggagccgtt caaataggct accttctctc tgccgccttc gggggccggg aagggcgaaa
     1741 ctccgtattc gaaatcgctc ttggcgaact gctttatgaa gagcagctcc cactgtccgg
     1801 cctcctggat cgccagctgt ccccggtaga aggggttgta ggaactggcc atctctcccc
     1861 aggtggcctg gaagaccctg atcttctgca gatcgaactt cttgtagtac gaagccaccc
     1921 aatcgagcgc cgcgacgttg ccgggatggt tggccgttat ctcttcggtt tccggatccc
     1981 acagctggcc gccgaagacg tagaagtaaa tatggctcac gttcggcaaa aagcccagcc
     2041 gccttatatt acccctggtg tcctgtttga aaagcagttc ggcgtacctg tcaagctcct
     2101 ggatattttc cggcggtctg tccgggtcga ggccgacctc tttgaagaga gccttgttcc
     2161 acatgaaggc gtaagcgttg agggtcgtag ggaggccgta cttgatcccc ctgtaggctc
     2221 cgtaagacca gaccccggga aggtagtcgg actcgtcgat ccctgccgag ccgagataat
     2281 cgttgagcgg tatcaaagcg ccgctctcgc ccagtggcac gaccatccag tcccagatcg
     2341 tcactatgtc aggcgtcgaa gaaccggcca gagcggtgtt tatcttctct ccctgtccta
     2401 tcggaacgaa gaccggcctc acgactatct cgttctggct cgcgttgaat tcctccacca
     2461 gtgccagaag ggccttccct tcgtcccctc cccaggcgta ccagaaggaa agctcggtct
     2521 tggccagacc gaccatcgca actgccagta taaccgtgat cgcaagaact ttcttcatga
     2581 tcaatccccc aatcctgtgt attctttata ggccttgtaa agctcttcgg ccttgcctcc
     2641 cagcctcgga gtcatgtagt gtgggaaatc gaatatgacg actttctcgg tgtagggaga
     2701 ctgcatctga agctggtcaa cgaccctttc tatatcggcc ggcacggcct gccagcttcc
     2761 ggcatcgacg ggccagccgt ggatctggtc gaagacctcc acgtttaccc agaacctcac
     2821 tccgtgcttg tccatgatcg gcttcaaagc ctttataacc ccggccgact cggcgaccga
     2881 agttcttctg gttccgactc cgtcctgcat ggctatgata tccaccttcg cctcgctgag
     2941 cacataatcc caccattcgg catacgcttc cccgttgggg ttgattccga agtatggaga
     3001 gatcatgacc ggctttctgg tgaggaactt cgcgtaggta accatggatt taagataagt
     3061 ccttatgccg tctttcctgg tctgcgtgag gaaattcctg tcgtctatct cttcgggaag
     3121 gtaccagccg gcaagagagg cgtgagcgcc gtaaagactg aaaagctccg tcaagatctt
     3181 ctggttcatg tttatctgtt ctttgggatc gaactcgccc gaccagtaag aggggttgag
     3241 cgagagaccg aggaaaactt tcatatcgag cgaatcggcg gccttcaaga gttcgccaag
     3301 cgtgtcgttt gccttttcct gtttgttctc ttcccaggtg accttccccg cgctcatgaa
     3361 ggccttcccg tcgtttgaaa cggctacttc gtagcccttc gaggggatct gaacaccgga
     3421 tatttccgat ctcatgaact tgacagcgat agagtctata gtggtcaact ctcccagatc
     3481 tacaacgatc tcgatgggtt tttccggata ctgccagccc accatcgcgc cccatgcgta
     3541 atcggccaca ccgtcggtca attttccgcc ggagggatag ctggggttgg cctggggatc
     3601 gaccgtgtag gtttttccgg ccgagagcgc ctcttccccc ttcttgacgg cgatctcggg
     3661 gatcatcgtc cattctgtgc tggtcggttc aattactatc ttcacgaacc tcgttctggt
     3721 cgaaccgctc cacaccagtt cggcgatact ttcgtcgggc gagatctcgt ctgttttcat
     3781 atacctggaa gggtaataga acctctcccc gtaagcgctg tactggatta ccaccgtgtc
     3841 catgcctata tccttcatga gagagagttc actcttccac tgttcctcgg tgaactggga
     3901 gagctgtcca tctagctgta tgaaagctcc tgttatattc ttgacctcgc cgctgcccgc
     3961 aaaggccgtc aggccagata ctactagcac gagaaagata gcgattacct tcattcgatc
     4021 aacctccttc aaagttagcc gtctttgtga atattctacc agaaattatt attttctcca
     4081 aaactaatag atagttttga gaaatactca caaacgatgg caaacaatgg cgaacgaaga
     4141 actcgaaaaa acgaaatggc agccctttga atctgatgag cagaatggaa ggggcctgaa
     4201 atagccgccc ttcgagagcg tttccaggtt gcagagtggg gatctatttt cttaagtgtg
     4261 cgaggtccac ggggtcattc agatagccga tagcgcctct atcgtttcga ctttctccgg
     4321 ttcgccggtt ctcaccgact cgaagatcga gagtatgacc agagaggtgt tcaccgcgtc
     4381 ttcgaggctt accaggaact cttcgccgct cgccaggcaa tcgatgaagt gccttatgct
     4441 ttcgtatgcg aatcccttcg cctttccgtg aacgaaatgc ctcacaagca catcgggccg
     4501 tttcgcttct ctctgtgtat agctttcgat catctggttg ttgctgagat cgaggttcac
     4561 cattccccgg cttccggtga agttgaactt gatatcgttc acgcaggggt tggtgttcgg
     4621 cgttatccag ctgttctcca tggtcgctat accgcccttt ctgaacttga gaagggtctg
     4681 gtagatgtcg gtggtgtcca gtcccatgtt tttaaggacg ccgccgctgc tcaccgaata
     4741 aacgctttcc acctcgtcgt tgaaaaacca tctcagagta tctaccgagt gactccccag
     4801 gaaccagagg atagacgagc gggccgacca cggcagcatg tcggtggcca cccagactac
     4861 gtcgttcaat ctgaagtagg cgctgacggg cgtgccgagc tctcctccgt cgagcatctc
     4921 tttggctacg gcaaagggag ggctccacct gttgtgaaga tcggtcatga tcctcacccg
     4981 gttcttcgat acggcgccga caatcgccgc aagatcctct ctggtggttg ccagcggctt
     5041 ctcaacgagc aggtctttac cggcgttggc acagtcgacg gcgattctgc cgtgggcgaa
     5101 gtccggcgtc acgatcgccg ccgcgtcgaa atctaggctt tcgagcatct cgatgtgatc
     5161 ggtgaagaag tgcggtattt cgaacttctg cgcgaagctc ctcgctctcg ataggtcgag
     5221 gtcgcagatc gctaccagct cgacggccgg atgttccctg tacagaaagg catgcgtctc
     5281 tccccagacg ccggctccga ttatcgccat tcttatcttc gacattctct tacctcctct
     5341 ctcagttcca gatcgcactc gaaagtcctg tcccagggca ggaagaagtc cgttacggag
     5401 atgcttccct tttctacgaa accgccgaaa acgtcgtgac gcctgccgta gaagtgtttt
     5461 tccatcaatt cgaccgcccg gggtatgaag agaccgtcgc ggaactcctc gaagagaagg
     5521 cccgaatcgc cgactctgta ttcgtcgcct ccgacatttc gaatcctctt ctcgagttcg
     5581 tctcgcaaga cgccctggta gaggtgatcg tccaggaggc gggagagata gaacgcggca
     5641 atctctctgc aggagtttgt agacctgcag gccgcaacca ccacggcgag caacgtcccc
     5701 agactgttgg aggcggtgtt ccacgccgaa tagccccaga tattttccag gcccatattc
     5761 aggaagccct ccacgaactg tcggttggag ccgttggccc tgaagacgtc tagtatgaag
     5821 attctcttct tctttttcga caaagcttcg agtttttcca gagctctctc cacttcgctg
     5881 ccggctataa agatcgaggt ttcggactca cccgagacgg tcaacccgac cagcctcatg
     5941 tgcgatccga cattttcccc gaactgtctg tcttcgaaat ccataatttt cttttccgtc
     6001 tcaggcgaat cgtaggtcag atctaccgac agcggttgcc ggtctgcaga tgtaaaagtc
     6061 aacaactcct ggataacctc gtcggcaccg ttgtgaatga agacactctc cttaacacca
     6121 ctctcgcgag agagtttgtc gagtatctcc agctcgcact tctgcggacc atcggggaag
     6181 gtgtcctcct gagcgagcac gagcagatcg gcgatctttt ccttgacaag ctccacacag
     6241 gctctgttga ctatatggtt cctgcttctg agtctgtaat aactctccag aaagcccctc
     6301 ggaaaggtag attctatcga tcgcgcctca tcttcttcgt cgtttccgat agcgcgtagg
     6361 taccggttca atctctccca gagttctctg gaaccggcgg aggagacaga gatcgaggcc
     6421 ctccttacta tcgaggagag aagtatcctg gagcggggat ttctctctct tagttcgcga
     6481 agcagttcca gccttttcaa agcctcgaat tcgctcacat ctccggctct tgaggcgata
     6541 agcccgccga agaggagcat atccacagat atcgcgaaag cgtcgccgct ctcttcaaga
     6601 agccatcggc caagctcttc gcaccttccc ggtctcatgt acctaccgag tagctcacgc
     6661 ccgggaaggc ggacttcaag tccgagcatt ttccctatct tctggactat acggtagttc
     6721 ggcggtcttt cgtccatagg aagaacgact gctttcacag aatccccccc attctcgcta
     6781 tctctttctg aatggtcttt attggcacct ctctcaccct gtaatgcgag cctttcgaca
     6841 gcgcggcgca tatgcccgcg gccatgccgg tcgccgaggc aagaggactg gtcctgagcg
     6901 cggaaaaggc gagatgctcg gcgctcaagg ccctcccggc gagaaccacg ttttcaagtc
     6961 caagtgggaa gagagccctg aggggcacgc tgtactctcc cggaaagggt attttcatgg
     7021 tcttcagctc acccgattcg ggaacgtgca gatcgaccgg ataagagccg atggcgatct
     7081 tgtccacgaa ttccttttgc ccgagcagat cgtcaacttt catcatgtac tctccggtca
     7141 cgtgggtagt ctctctcact cctatactgc atcctgtctg tatgagagac gcattcgcga
     7201 aacccggcac ttcactcttc aagaaatcca tgaatttcca cacgtcgatg tttccgtcgc
     7261 tctgggcggc ggtcagttct acggcgctac ccccgaatcc tgcatagcga gtcgtgttca
     7321 tgaacacctc tccgggatgg aagggtattt cgaagaagag taacctgtcc cttctgaagt
     7381 tgaccctgta attgcgcaga agcggcatta gactgaagaa accggcgact gcgaggtagg
     7441 agaaatccct cttttcaccc agcacgaaat ccgattcgtt ctcgccgatg aaagagatga
     7501 tctcgtccct gtttattccc ccgattctca tcaccagagt catgggctga caattggccc
     7561 tgttaccgtc gccgttgacg tatctggccc cggctttgat cgagaggttt ccctctccgg
     7621 tggcatctat aaacgcccgc cctatgaagg tgcggagttc attggcgttc accgtctcta
     7681 cccgctgcag gaatcccttt tctacccaga ctctgccgac cagcgaattg aatagatagt
     7741 cgactcttgc ctccctgagg agctctatca aaacactctt catcttttcg ggatcgaagg
     7801 gtgtaatact ttttacgaaa ccgatcggat cttccagatg tccgtgggag cctctttccc
     7861 tgaccagtct ctccactatc tcctgaccta tacctccgat cacccgtctg cggtgggagt
     7921 ggaaggtcat catggggtta accagcgagc cggtcagaac tcctccgaaa gacatacctt
     7981 tctcgattag gagaaccccg gcaccgagcc gggatgcggc gaccgcagcc gaaacaccgg
     8041 ctatgccacc gcccacgacg attacatcgt aaactctatc cattgtttca ccctctcatt
     8101 ttattccaga actcaaagcg gtatcggtga agtaacgttg aaagaccacg aagatcaaga
     8161 tgaccggcag gatcgacacg gtggttgcgg ccatgagcgg tccccagtcg gcggtgtacc
     8221 tgtttttgaa gacctccagg cccagctgaa gggtacgcat gtttctgtcg cttgttacca
     8281 ccagaggcca gagaaaatca tcccacgcct gcgtgaaggt gaatatcgcc agcgccgaca
     8341 tggccggttt gcccaacggc aggaagatcc tagtaaatat gcttatctcc ctggcgccgt
     8401 cgatcctcgc ggcttcggag agctctctgg gaaactgcat atagaactgc cgcattatga
     8461 agacgccgaa cacactcatt atgtggggga caaccagacc ggcgtaggtg ttcaaaagac
     8521 cggtgcctcc ctgtccgaag atgttgttgc cccctgccag cggaaaggat ttaaccataa
     8581 ggaaggtagg aatcagcgtt acctgaaccg gtatcatcat cgtgctcaaa agcagcaaaa
     8641 agagaaagtt cttgagcggg aagtccagtc tcgcgaaggc gtagcccgcc agtgaacaaa
     8701 agacgagatt gaaggccata gagatgagcg agactacggc gctgttgaga aagtacctct
     8761 cgaatccgcg cgtcgttagc gccgtcgtgt aatggtcaag ggtcagatgg cgcggcacga
     8821 acgtgggagg ccacgagaag agctcggcgt tgggcttgag cgaggaaatc gccatccaga
     8881 agaagggcga tcccatcacg acggccgcta tcaggaggag gaagatgacc ctcaggcgtt
     8941 taaacttctt cattcggcca cctccttttc acgccgccag gtgaaactca caaccgagag
     9001 cgtcattatt attaatccga gaatgaaaga catggcgctg gcataaccca tgtccatgta
     9061 atcgaaagcg gcgcggtaga tgaggaggcc gacggtcgtc gtactctcca gaggcccgcc
     9121 gctggtcata acgtacattt ccgtgaacga ctgcaacgtg ctgataagac cgacgatgag
     9181 aacgtagata tgtaccggcc tgaggagcgg cagggtgatc ttcaggaatc ttttgaaggc
     9241 cgaggcgccg tctatggccg ccgcttcata aagatttctc gagattccct gtagccccgc
     9301 cagatatatg agcatattcc agcctgctcc cctccagact ctcatcgcta ctatagaatg
     9361 catcgccgtc gcggggcttc gaaggaagtt ctgcggcgga atgttgaaca atgaaagcag
     9421 aaaattcaag atcccgatct ggggatcgta cagccatagc cagatcatgc tggcggccac
     9481 catgggcgtc actacgggca gataatagat agccctgaaa acgctcatac ccttcaggcc
     9541 ggtgttgacc agaagggcca gcaggagcga cagcaacata ttcagcggaa gtacttcgac
     9601 ggaaaactgt agcgtcctca gtaccgcgct ccagaattgc gtgtcgccga agattctcga
     9661 atagttgaca aggccgatca gtttcgccga cccgaagccg gaaaacttcg taaagctcaa
     9721 ataaagcgat acgaagatgg gcgctatcct gaagatgata ttgtaaacga gtaccggtga
     9781 gataaaaatg aacgcggtca gcatgatccg ctttttcagc ctgttagtca cctttcaccc
     9841 cgcaaggtcg tcacgatcga aatactgccg gcctttcggc cggcaggact ttacttgagt
     9901 ttactcactt ctttggccgc ttcgtcgagg gcgtctttcg gattcattgc acccatgacg
     9961 gctctttcaa ggtatttgga gatgatatct cttgcctgga cccagaaggg aacgttcggg
    10021 ttgccgtagg cgtatttggc tccctcgacg aacttcttga gatcggggtc gttcgcgatg
    10081 aagtcagcct gtgcgcccga ctgtctcggc ggaaggcttc caagggcttt gttgatcttc
    10141 acgagcgaat ccttggaagt caggaaggcc agagccttcg tggtggcttc gaggttcttg
    10201 gtgctgctgg aaaccatgaa acccgcgaag gagtagaatc cggccctggt gtacctggca
    10261 ggggggaagg ccagcctgat tttcgagtaa gattccggac tgtactcctt aagggcggcc
    10321 agcgactcgt tgttcatgaa gaccatgacg gccgtacccg cggcgatcgg ctcgagattg
    10381 ccgactggag tcagtccgaa ctcggaagcc actttggcct cgcgcacgag cccgacgtag
    10441 tattcgagag cttccacgcc ggccgggctg ttgaacaccg gattcagttc atcgtcgaag
    10501 agtttgccgc cgttctctat aaggaagctg gtccagacct gctgtaggtc gatgccggtt
    10561 acggggatat cgagaccggc cctgactatt ctcccagaat cgtaaatcgt ggtggctttt
    10621 gcaaatgtca ccagctgctc ccatgttacg ggcggctcgt tcggatcgag tccgctctgc
    10681 tcgaagaagt cggccctgta agccagaagt cttcctgaac cgaacacggg gacgaaataa
    10741 ttcttaccct tataaatacc cacatcgaga gccgcgccga aatcggccgg atcatcgagc
    10801 ctggcgatga gatcgtcgat gctccttatc acgccgttct cgacgaatcc agcgatggcg
    10861 gcctgaccgt gcatgaatat atccggtcct atcccgccgg cgaaggccgt agtcagcttc
    10921 gtggagagct ctccccaggg aatgtactcg acaacgagtt tcaggtcggg attcgccttc
    10981 tcgaacgccg gaattagatc gtcgtccatg gcgatcgtga gctgatccga agtgcccgcg
    11041 aaccatacgg tgacgtttgt tttgccaaac cctacaaaga ctaacagact taaaaccgaa
    11101 agaaccagta ctagcttttt cacttccaca cctccccttc agtatcggat tgcttcgctg
    11161 aacgatttaa caacgcttga aaagctcttg atatcagtac cggtaactat ggggccgatt
    11221 atgaggcccc tgaagccaat ctcgaaaagc tccttgactt cttcgggcat tactttcttc
    11281 tgcgtgggta ggaaacaggg aagaccggcc cgctctataa cttcacggta tctcagaagg
    11341 tccataagcg tgagagggct gccgtacttc tcggggttga ccgtagagac ctctacgcaa
    11401 tcggcgccca gagatttcaa gttgccggcc atcgagggat cgtaagtgta gttcagagcc
    11461 agcatcttcg acatcccgtt cctcagcagg tagagcggag cgtaatctac ataaacatcg
    11521 aagaaagaga tgcccgattt ctccatttcc tcgatctcgc cggcgccgga catattttcg
    11581 gctcccggca ctatacccat gcatggatcg aactcggact tgattcttcc aaccgtctct
    11641 cttataccgt cccacgtagc gtggagtttg ccggtaaccc tgtgtttaac gttgacatga
    11701 agctttaccg cgtcggctcc gttttccagg gccgcccggg cgaactcgaa cgtattttcc
    11761 ggcagactga ctatcagcaa tttccgttcc gatttcagca gttgtgagaa aatcattttc
    11821 agcccccttt cagcgtttcg cctttcagaa gataaggttc aacaaaaacc acgcccctgg
    11881 cctcgtcgtt accttcgagc agctccgaca gcatcacggt ggcgatcttt ccaagcagat
    11941 aaggattgag cgcgatcgtc gtcagtcttg gccttattct tctggtgaag tccagtccgc
    12001 cgaaaccgat aaccccgacc tgcccgggaa tggctacctc ctgttcaaag aaggcttcca
    12061 taactccttt tgccagccag tcggttgtac agaaaatagc ttcgcgtctt tcggacttcc
    12121 tgtactccct ctcaacgaac ctctttccat ccctctcttc gaaggagttg cactcgatga
    12181 ccctcacgtt ttctcctgga taattgtttt ccttcagaca cttgaaaaag ccttcgagcc
    12241 tgtcggcgaa ggtcgaaacc tccctgggcc cggtaattac ggtgatcttt tcgtagttat
    12301 ttttaagcag ttcatacgcg gcgagatagc cgcccctgac gttatcgttg ttcaccgaag
    12361 gaaaactctc gaggccgcag tatttgccta cgagaacggt cggtattttg agagacatga
    12421 gaaaatgtat catagattca ggcatcggga tgcctcccac gaccaaccct tcaaccagat
    12481 cgacgggaat aagcgactcg tcgaaggcgc tgcggcttct catcgattga accaggcaat
    12541 gaagaccttc cccgctggaa gtctggagaa tgcccctctg cacgcctgag aagaactcgt
    12601 cgctctcgat cgatctgtgg ggattgctca cgaagaacat gatcactctg ttgcttgata
    12661 tgctgccagg ctcgactgga tggcccagct tcctggcaac tctgagaacc ttctccttcg
    12721 tgctctcgct tatccttgga tcgttcttta agacgcgtga aaccgtggag atcgaaactc
    12781 cagccctggt agaaatttcc ttcaaggtca cttccatggc acaactcctt tatgcaaatt
    12841 gtgcaaacag actgcaaccg aaacattgta gcacctggaa agaaaataaa taaacccaaa
    12901 agaacctgcc ctggtgtcac ctgagctaat catctccgat taagacttat tattcagcgc
    12961 gatctatact tatctgaggg catcgcttca aaatatacgg aatagaaagt attgcaaatt
    13021 atgcaaagag tcgtccgttg ttcgtgaaac acaagagaaa gaacgtcgag agttcgccct
    13081 ctttctctct ctttatctct ctttctctct ctctctttat ctctctttct ctctctctct
    13141 ttctctctct ctctttctct cttctctctc tttcacctct tctcgttctt cccaaggacg
    13201 gctctttacg aaaattgcgt acagacctca ggagccggtc agtcccccat tttcgaaaag
    13261 aggtaagagg catgaggtta gaggttaga
//
LOCUS       DAOOLY010000039        13123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5228_length_13123_cov_4.276094, whole genome shotgun sequence.
ACCESSION   DAOOLY010000039 DAOOLY010000000
VERSION     DAOOLY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_13123_cov_4.276094"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PLD15_03355"
     CDS             <1..1306
                     /locus_tag="PLD15_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62968.1"
                     /translation="LISSCFRLPSTPLRYLRFTFSPREPFLEIVPGVKLSLKDTEKTG
                     TLAYAVQRPSGDSLLLIFEDGSPLSGMIMELSHELLGAGVSQVLMVQPLLRDGTFSPA
                     DSGCEIAVSRDALETVVRFTQLPYCLLISWADALYFAQHLPGKYLLEGDLLYHIGPNW
                     LPSHSGIYLGVDRETAMSNENYGSNDGVTFADSYPYQTHGFNLIEEGVFVFPNDLDGS
                     RYVNLFNYDTYASRWLKYWAPAFTGPRRYAGELTDEQRRKISRYLYRTADNGALWSVG
                     LAWSGYRDLPFTKRDLYSCVGIVEKAYESAGANIVPFWDDWFYLNSFEEFCRTIAIPS
                     ISEYEGNTVEFRVNALLADWQYVGSTAWYVPEFAWVWRSTAGVELVDSPGTLIAKGDH
                     CIYSWTPPRAGVYEVTFRFHGLFEGQPVEKSHTLEINVLQKR"
     gene            complement(1294..1629)
                     /locus_tag="PLD15_03360"
     CDS             complement(1294..1629)
                     /locus_tag="PLD15_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPB62969.1"
                     /translation="MRMEEKETSIYRRIYETVKMIPSGKVATYGQIARIVGSCTAIMV
                     GYAMAGVSDPEVPWQRVINSKGKISIRDHAGYSLQKAILEREGIEFDESDTVDLSVYG
                     WRGPELYLF"
     gene            complement(1727..2269)
                     /locus_tag="PLD15_03365"
     CDS             complement(1727..2269)
                     /locus_tag="PLD15_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB62970.1"
                     /translation="MKEHLLVAAGFGGQGVMLLGQVIATAGMFDGKYTTWLPSYGPEM
                     RGGTANCTVVINDEPIGSPITESPNELIVMNIPSLTKFEKQIQPGGVMVINTSVVDRE
                     STRKDIEIFKIDAGAVAERLGNMKVANMVILGAYIAKTGAVTLEGVRKAFEKKLTGEK
                     ADLIDLNIQAVKAGMEAVGQ"
     gene            complement(2266..3018)
                     /locus_tag="PLD15_03370"
     CDS             complement(2266..3018)
                     /locus_tag="PLD15_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB62971.1"
                     /translation="MTEATYKRPESLAKREFSYCPGCHHGIIHRLVAETIDELSIREK
                     TLMVAPVGCSVFAYEFFDVDGTVAPHGRAPAVATGMKRSLPDRVVFTYQGDGDLAAIG
                     TAEIVHAANRGEKITTIFVNNAIYGMTGGQMAPTTLLGMKTTTSPYGRKSETEGFPLH
                     VSELLKETAGIVYLERVAVSSPQEVRKAKAAIKKAFTVQLKGLGFAMVEVLSTCPTNW
                     GLSPVESIKWLNENMKKEYPLGVFVDKVGDLK"
     gene            complement(3028..4083)
                     /locus_tag="PLD15_03375"
     CDS             complement(3028..4083)
                     /locus_tag="PLD15_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPB62972.1"
                     /translation="MAEKVMVKGNEAMAEAAVRAGCRIYFGYPITPQSEFIEYMARRM
                     PQIEGVFLQSESEVAAVNMVYGAACTGNRVMTSTSSPGFSLMQEGVSYIAGAMLPAVF
                     VNVVRGGPGLGDIQPAQSDYFQATKGGAHGDYHLIVLAPGSIQESVDLMEDAFRMADE
                     YRMPVLILADGMLGQMMEPVEFPEFVKLEGINDHSAWALRGATGREANKVTSFDIDPV
                     NLEKMNIFIQEIYKRIKAKEVRYETIGLEDAEYMLVGYGTMGRILGSVVKLARSKGIK
                     VGLFRPITLWPFPYAELKKAAEGVKTILTVEMSMGQMVEDVRLAVEGSVPVQFYGRTG
                     GMVPTPDEVLAELLKHV"
     gene            complement(4096..4320)
                     /locus_tag="PLD15_03380"
     CDS             complement(4096..4320)
                     /locus_tag="PLD15_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPB62973.1"
                     /translation="MSKNFVKIDEERCKGCGLCINFCPKKVLSFSEKFNSKGYHPARQ
                     HDPDNCIGCGFCYMMCPDTAITVFKETAKV"
     gene            complement(4313..4987)
                     /locus_tag="PLD15_03385"
     CDS             complement(4313..4987)
                     /locus_tag="PLD15_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobQ"
                     /protein_id="HPB62974.1"
                     /translation="MEFPGKILIFMGMFGSGKTEIALNFAQSLSKAGQRVALADIDTI
                     SPYYRSRDLREAFGKDGIKIIAPAGKLAHADLPIISPEIFGYVENPEYRIVIDVGGND
                     DGAVVLSSLSRRLPAERCTTFYVLNPYRPFNDTIANAVSHVERLREKSRMRIDFLVNN
                     SNIGPETTGKEILHGEDFIEEISKITTIPVAFTVVMNGVKALKGRYHEFRIVKYMKNP
                     WEVENE"
     gene            complement(4971..6059)
                     /gene="buk"
                     /locus_tag="PLD15_03390"
     CDS             complement(4971..6059)
                     /gene="buk"
                     /locus_tag="PLD15_03390"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPB62975.1"
                     /translation="MPIVLVINPGSTSTKLALFNDRELIREHTIRHQPEELAQFNTLY
                     EQRLFRKRLIVEFLQSSGYPLDGIDMIVGRGGMLKPLQGGTYHVNDSMIEDLKTARYG
                     EHASNLGAILARELLSESGRDIPIFIADPVVVDEMEPLAKYSGHPDFKRRSIFHALNQ
                     KAVARQVAARYGKRYEEMNLVVIHMGGGIFIGAHRKGKVIDVNNALDGDGPFSPERSG
                     TLPLTGLIKLCFSGTYTKDEMKKLIKGRGGLMAHTGTTDCQKVQTYIRQGDQNSKMAY
                     RAMAYQIIKWAGKMAAVLRGEVDSIIITGGIAYDKNFMIPWLTEKLSFIAPIDIVPGG
                     NEELALAMAGLRVIEGSEEAREYDGISR"
     gene            complement(6060..6959)
                     /locus_tag="PLD15_03395"
     CDS             complement(6060..6959)
                     /locus_tag="PLD15_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPB62976.1"
                     /translation="MITNLSTLVELAKKNPKVVSVAAAEDEVVISSAREAYLEGIATF
                     RFYGSADKIEKMCSALNFKEGYTIIDCKNKAESIKSAILSVSSGESQLLMKGYIKTGE
                     LLSAFLKDEFNLKTGRTMNLVTVFELERYHKLLLVTDAGMVISPDLQQKADSIVNAAS
                     VARALGIEVPKVAVLAAVEVVNPKMQATLDAAVLSQMAARGQLGMVEVDGPFALDNAI
                     SAEAAKHKGIVSPVAGDADIVIMPDIEAGNIFYKAMAFLSGAELASTIVGGKVPIILT
                     SRADSERTKLQSIALNVVLAGGV"
     gene            complement(6956..8017)
                     /gene="buk"
                     /locus_tag="PLD15_03400"
     CDS             complement(6956..8017)
                     /gene="buk"
                     /locus_tag="PLD15_03400"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPB62977.1"
                     /translation="MKVLVINPGSTSTKIGVFEDSTLVLAESLKHVKREDSPHDKGVA
                     SRADEIRSFLAVKEISLEQFDAIACRGGILPPMESGTYRVNEEMCQYLVHSSKMDHPA
                     NLAAPIGMELSRGSIPVFITDPVSVDEFSPEARYSGLPQIPRVSLLHALNMKAAARQV
                     AAEFGRDLENLNLVVVHLGGGISVGLQLRGKMVDVNNANDEGPFSPTRSGELPVGSVV
                     EACFSSGLSEKELLSRYTKAGGMVAYLGTDDLRIAMEMARSDEKAREVIEAMAYGIAK
                     EIGGMCTVAGGQIDAIVLTGGMAYNGSFVEMIKGYVSRFALVVIEPGENELMSLAMGA
                     ERVLTGVEKAREFHPEVVL"
     gene            complement(8030..9259)
                     /locus_tag="PLD15_03405"
     CDS             complement(8030..9259)
                     /locus_tag="PLD15_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPB62978.1"
                     /translation="MTDKFSKMAKKMKSNIIRELLKVTGKPGMISFGGGVPDPETFPR
                     HEMAEISREILENEYRYTLQYASTEGDPILKEEYISLLKRENGIEGLELDNLLITVGS
                     QSALDLVSKIFLDEDSIYFVSKPVYLGAASTFSLRSRQYVYMNLTEDGVDLDELEQSL
                     EEIKVRGDIGKVKFIYVISNFHNPGGVTMSLAKRKRLVEIAEKYDVLIVEDDPYGDLR
                     YDGEAIDSVFKIGGKDRVLLLKTFSKILSPGLRLGVVIGSHDVIRKMVMAKQAADLCT
                     PSLTQRLAARYLQRHDIMAQIKPTIALYKEKKDLMLAELEKNFGDMEGFHWTRPDGGL
                     FIWFTLPEGFNTDEMLEMGKSENVLFVPGIAFSLDDTCKNSMRLSFCLPPKNDIIEGV
                     RRLKKVIMEYGEERNLL"
     gene            complement(9468..10850)
                     /locus_tag="PLD15_03410"
     CDS             complement(9468..10850)
                     /locus_tag="PLD15_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62979.1"
                     /translation="MKRLFLILMFLIVTVFAFSGPGFFISFFPVLSLNNLPAHDRDYR
                     PTMYDMYDYFTYPAFATFGIELSGPSYRALLRFDFRQDLSAYLRGKGWSNLPLDPNKN
                     SLYIDTDLPRVGLAEYENSLIRLSAGKRKLHFGPATYNFVLSETVPYFEHLWFDLNTE
                     VSYGRYFYNFFLISSDRSVYNSPKTLIGHSFGFSNELLRLGLVETNLISKTFPDLRDL
                     SPFTIFHNNYARNSNVNAGIFLELDLDRFDLYGLLYADDILIPGDRTKNPTSLGWYAG
                     GTYTFIDGGNYSGPLLWDSQFTLREKTVFENSGGLKLKYEHYHSTPYLYNRDFEEGKY
                     TNPIRFNAGDIEGGQYVVVNGFYGFPYGPDVTLDLLALSYETEKVLTEIRLEYLRSGV
                     YKIDNYYGEPFEYDWYRLAEPITRKALLSFNLHYSPDGRQEFLFSSLLSFGEKLGFNF
                     KAGYGRFFSF"
     gene            complement(10913..11524)
                     /locus_tag="PLD15_03415"
     CDS             complement(10913..11524)
                     /locus_tag="PLD15_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPB62980.1"
                     /translation="MGRIIVFAGNVGAGKSTIAGAVAERLRYGVHFESVSDNPFLEDF
                     YYDQKRWSYHLQTYFLYHRYCSLKHAEANENMVFDRSIYEDAEIFARNLFETGKMSRR
                     EYDAYTTMFYAMIDYLKNPDLLVYIDADIDTILTRIRRRGREMELAVPIAYWEQLDRL
                     YSDWIGSYNRSPVYRIDARIVDIVTNPGHLDGVMRDIEAIING"
     gene            11788..12243
                     /locus_tag="PLD15_03420"
     CDS             11788..12243
                     /locus_tag="PLD15_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62981.1"
                     /translation="MKKLLLLFVVLGLAVALFSEPLIVWPDKAHGKPLVAGLHSPVYG
                     EAKLDVFGNITGWTGPNLGLGWTWKTYFSPLELQKVNFYYEFGTNLVIFPYIGAGFDY
                     ALVLQNNQTLLLGAGIAASPLQVLSAILAPTTIPASIISAIKLSVAVVF"
     gene            12293..12706
                     /locus_tag="PLD15_03425"
     CDS             12293..12706
                     /locus_tag="PLD15_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62982.1"
                     /translation="MSEDLSSKQEHDSEVRSTITKYSIILGIALAIILILLSTRGRVE
                     SFDPESTIGTIERTAELFIKERLLDEGESLERIEIVKVNYSTVEWGEYTYEATIQYRT
                     LSGEETLFTGWKYSVKSDSLDLLLYSRGDGPPVEP"
     gene            12836..>13123
                     /locus_tag="PLD15_03430"
     CDS             12836..>13123
                     /locus_tag="PLD15_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62983.1"
                     /translation="MKKLLILVAVLMVASIFVAAPKNFILGQGLYGDVVYDMMGNITG
                     FEGMDFFLFQASTYYEKPITPNEVNAFTFDGKFLFFIPVFGRGWEFLLRPAD"
BASE COUNT         2945 a         3495 c         3231 g         3452 t
ORIGIN      
        1 cctgatttct tcatgcttca ggctcccctc gaccccgctt agatacctca ggttcacttt
       61 ctcgccgcgg gagccttttc tggagatcgt tcccggcgta aagctatctc tgaaggacac
      121 ggagaaaaca ggaacgttag cctatgcggt tcagaggccc tccggagaca gtcttttgct
      181 gatcttcgag gatggcagcc ccctttcagg aatgatcatg gagctctctc atgagttgct
      241 tggagcggga gtgtcacagg tgctgatggt gcagcctctg cttagagacg gtaccttctc
      301 gccggccgat tccggttgcg agatcgccgt gagccgcgat gctctggaaa ccgtggtgag
      361 gttcacgcaa cttccctact gcctgctaat aagctgggcc gacgctcttt acttcgcaca
      421 gcatctgcca ggcaagtacc ttctcgaagg agatctgctg taccacatag ggccgaactg
      481 gctcccctca cactcgggaa tatacctcgg agtcgataga gagacggcca tgtcgaatga
      541 aaactacggc tccaacgacg gcgtgacctt cgccgattcc tatccctacc agacccacgg
      601 tttcaacctc atcgaagagg gagtcttcgt ctttcccaac gatcttgacg gttcgagata
      661 cgtcaacctt ttcaactacg acacatacgc ttcacgatgg ttgaaatact gggccccggc
      721 cttcaccggc cccagaagat acgctggaga gctcacagat gagcaaagaa ggaagatctc
      781 gaggtacctc tacagaaccg ccgataacgg tgccctctgg tcggtcggtc tggcctggtc
      841 gggttacagg gatctcccct tcacaaagag ggacctttac tcttgcgtag gcatagtcga
      901 aaaggcttac gaaagcgctg gagcgaacat agtgcctttt tgggacgact ggttctacct
      961 caactccttc gaggagttct gcagaactat cgcgattccc tcgatcagcg aatacgaagg
     1021 caacactgta gaattcaggg tcaatgccct gctggccgac tggcagtacg taggatcgac
     1081 cgcatggtac gttccagaat ttgcctgggt ctggcgatca acggccgggg tcgaactggt
     1141 cgattcgccc ggaaccctga ttgcaaaggg agatcactgc atctacagct ggactcctcc
     1201 tcgggccggg gtttatgaag tgaccttcag gtttcacggg cttttcgaag gacagccggt
     1261 cgagaagagc cataccctcg agataaatgt gcttcaaaaa agatagagtt cgggcccccg
     1321 ccagccgtac acggaaagat cgacagtatc gctctcgtcg aattcgatgc cttcgcgctc
     1381 cagaatggct ttctggagag agtaaccggc atgatcgcgt atgctgatct tgcctttgga
     1441 gtttatcaca cgctgccagg gaacttccgg gtcgctaact ccggccatgg cgtatcccac
     1501 cattatggcc gtgcaactcc cgacgatcct ggctatctgc ccgtaagtgg caactttccc
     1561 tgaaggtatc atcttgactg tttcatagat ccttctgtaa atggacgtct ctttctcttc
     1621 catcctcatc accccgaaac tccacaaaca tttttaaaga aaccggcggc ggacgggtga
     1681 gcgcccgctg ccgggaaata gcaaggaggt accggagccg tcgcccttac tgtccgacgg
     1741 cttccattcc ggccttgacc gcctggatgt tgagatctat gaggtctgct ttctcgcctg
     1801 tgagcttctt ctcgaaggct tttctcacac cctcgagcgt aacggcgccg gtctttgcga
     1861 tataggctcc aaggatcacc atgttggcca ctttcatgtt tccaagtctt tcggccacgg
     1921 caccggcatc gatcttgaag atttctatgt cctttctagt tgattctctg tctacaaccg
     1981 acgtgttgat taccatgact ccacctggct gtatctgctt ctcgaacttt gtcagcgagg
     2041 ggatattcat cacgatcagc tcgttgggac tctcggttat cggggaacct atcggttcgt
     2101 cgtttatcac taccgtgcag ttggccgttc ctcccctcat ctcgggaccg taggagggaa
     2161 gccatgtggt gtactttcca tcgaacatgc cggccgtcgc tataacctga cccaggagca
     2221 tcactccctg tcctccgaat ccggcagcca ccagaagatg ttctttcatt tcagatcacc
     2281 gaccttatcg acaaaaacgc cgagagggta ttcctttttc atattttcgt tcaaccattt
     2341 tatcgattcg accggactca aaccccagtt cgtcggacag gtggaaagca cctcgaccat
     2401 cgcgaagccc aggcccttga gctgaactgt gaaggccttc tttatcgccg cttttgcctt
     2461 tctcacttcc tgtggagagc ttaccgcaac tctttcgaga taaactattc cggccgtttc
     2521 ctttagcagc tccgacacgt gaagcgggaa gccttcggtt tcggattttc taccgtacgg
     2581 ggaagtggtg gtcttcatcc ccagcagagt ggtgggagcc atctggccgc ctgtcattcc
     2641 gtagatcgcg ttgttgacga agatagtcgt tatcttctct cccctgttgg cggcgtgaac
     2701 tatttcggcc gttccaatgg cggcaaggtc accgtcgccc tggtaggtga agaccactct
     2761 atcgggcaac gagcgtttca ttccggtggc taccgccggc gcccttccgt gtggcgcaac
     2821 cgtaccgtca acgtcgaaaa attcgtatgc aaagacagag cagcccaccg gtgcgaccat
     2881 caacgtcttt tctcttatgc tcaattcgtc tattgtctcg gctacaaggc ggtgtattat
     2941 accgtgatgg catcccggac agtaggagaa ctctcttttg gcaagtgatt ccggtctctt
     3001 gtaagtcgct tcagtcatat catcacctca tacatgctta agtagctctg cgagaacttc
     3061 gtcgggcgtc ggaaccattc caccggtcct tccgtagaac tgaacgggga cggaaccttc
     3121 gacggcgagc ctgacgtctt ccaccatctg tcccattgac atctcgacgg tgaggattgt
     3181 tttaacacct tcggcagctt ttttcagctc ggcgtaaggg aagggccaga gcgtgatcgg
     3241 tctgaagaga ccgactttga tgcctttact tcgggccagc ttcactacgc tgccgaggat
     3301 tcttcccata gttccgtaac ctacgagcat atactcggca tcctcgaggc cgatcgtctc
     3361 gtaccgtacc tcttttgcct taattctttt ataaatttct tgaataaata tattcatctt
     3421 ttccaggttc acgggatcga tgtcgaaaga agtaaccttg ttggcttcgc gccctgtcgc
     3481 tccccgaagt gcccaggcag aatggtcgtt tatcccttcc agtttgacga actccgggaa
     3541 ctcgaccggt tccatcatct gtccgagcat accgtcggcc agaataagca cgggcatgcg
     3601 gtattcgtcg gccattctga aggcgtcttc catgagatct acggactcct gaatactgcc
     3661 gggcgcgagg actataaggt ggtaatcgcc atgggcacct cccttggttg cctggaagta
     3721 gtcgctctgg gccggttgta tatctcccag ccccggacct cccctcacga cgttcacgaa
     3781 cactgccgga agcatggccc cggctatgta ggaaaccccc tcctgcatca aactaaaacc
     3841 gggactggaa gtggatgtca tcacccggtt cccggtgcag gcagccccgt aaaccatgtt
     3901 gacggccgcg acctcgctct cggattgcag gaatacaccc tcaatctgtg gcattctacg
     3961 ggccatatat tcgatgaact cggactgggg ggttatggga tatccaaaat aaatgcggca
     4021 accggccctt acggcggcct cggccatcgc ttcgtttccc ttgaccatca ctttttcggc
     4081 catgttatca ctccttcaga ccttcgccgt ctctttgaag acggttatag cggtgtcggg
     4141 gcacatcatg tagcagaaac cgcacccgat acaattatcg ggatcgtgct gtctggcggg
     4201 atgatagccc ttgctgttga acttttcgga gaaactgagt actttcttcg gacagaagtt
     4261 tatgcaaagg ccgcagcctt tgcaacgctc ttcgtctatt ttcacgaaat tcttactcat
     4321 tctctacctc ccaaggattt ttcatgtatt taactatacg aaactcgtgg tatcttcctt
     4381 taagggcctt tacaccgttc atgacgacgg tgaaggctac ggggatggtc gttattttcg
     4441 agatctcctc gatgaagtcc tctccgtgca gtatctcttt tccggtcgtt tccgggccta
     4501 tgttggagtt gttcacgaga aagtctatcc tcatcctcga tttctccctt agcctttcga
     4561 cgtgtgatac ggcgttggcg atcgtatcgt tgaaaggtct gtagggattc aaaacgtaga
     4621 aagtggtgca cctctccgcc ggcaacctac ggctcagaga cgatagaacc accgcgccgt
     4681 cgtcgtttcc gccgacgtct ataacaattc tgtattcggg gttttccaca taaccgaaaa
     4741 tttcgggaga tatgatcgga agatcggcat gggccaactt tccggccggt gcgattatct
     4801 ttatgccatc ttttccaaag gcttccctga gatctctaga tctgtagtaa gggcttatgg
     4861 tatctatatc ggcgagcgct accctctggc cggccttcga aagcgattgt gcaaaattga
     4921 gagcgatttc cgtctttcca ctcccgaaca tacccatgaa tatcagtatc ttaccgggaa
     4981 attccatcgt actctctggc ctcctctgaa ccttcgataa ccctgaggcc ggccatggcg
     5041 agagccagtt cttcgttccc acccggtacg atatctatcg gagctatgaa agacagcttt
     5101 tcggtgagcc atgggatcat gaaattctta tcgtaggcta tgccaccggt gattattatt
     5161 gaatccacct cgcctctgag aacggcggcc atcttcccgg cccatttgat gatctggtag
     5221 gccatcgctc tgtaggccat cttgctgttc tgatcgccct ggcgaatgta cgtctggact
     5281 ttctggcaat ctgtcgtacc ggtgtgggcc atcaatccgc ccctgccctt tataagcttc
     5341 ttcatctcgt cttttgtgta agtgcctgag aagcagagct ttatgagacc ggtcagcggc
     5401 aaagtccccg atctttcggg gctgaaagga ccgtcgccgt cgagagcgtt gttgacatcg
     5461 atgacctttc ccttcctgtg ggcgcctata aaaatgcctc cgcccatatg aatgaccacg
     5521 aggttcatct cttcgtacct tttgccgtac ctggcggcca cctgtctcgc gacggccttc
     5581 tggttgagcg cgtggaatat cgatcttctc ttgaaatcgg gatggccgga gtatttggcg
     5641 agcggttcca tctcatcgac cacaacgggg tcggcgatga agatcggtat gtccctgccg
     5701 ctctcgctca aaagctctct ggccagtatc gctccgaggt tcgaggcatg ttctccgtaa
     5761 cgggccgttt tcaggtcctc tatcatactg tcgttcacat ggtacgtacc gccctggagc
     5821 ggctttagca ttccgcccct tccgactatc atatctattc catcgagcgg atagccgctg
     5881 ctctggagaa attcgactat caatctcttg cggaagagcc tctgctcgta gagagtgttg
     5941 aactgagcga gctcttcggg ctggtgtctt atggtgtgtt ccctaatgag ttcgcgatcg
     6001 ttgaaaagcg cgagcttggt agatgtggag ccgggattga ttaccaggac gattggcatt
     6061 catacccctc ccgccagcac gacgttgaga gcgatggact ggagtttcgt cctctcggag
     6121 tcggctctgg atgtcagaat tatcggcacc ttcccgccca caatcgtact tgccagctct
     6181 gcacccgata aaaaggccat agctttgtag aagatgtttc cggcttcgat atcgggcatt
     6241 ataactatgt cggcgtcccc cgctaccggg ctgactatac ccttgtgctt cgcagcttcg
     6301 gcggagatcg cgttgtccag cgcgaaaggg ccgtcaacct ccaccatgcc cagctgaccg
     6361 cgggcggcca tttgagagag tactgccgca tcaagagtgg cctgcatctt cggatttacg
     6421 acttcaaccg ctgccagaac ggcaaccttc ggcacttcaa tacccagcgc tctggcgacc
     6481 gaagccgcat tcacgatgga gtcggctttc tgttgcaggt ctggagatat caccatcccg
     6541 gcgtcggtga ccaggaggag tttatgatat ctttccagct cgaagacagt caccaggttc
     6601 atggttctgc ctgtcttgag gttgaactcg tctttgagaa aggcgctcag aagctccccc
     6661 gtttttatat accctttcat gagaagctga ctctcgccgg aggaaactga aagtatcgca
     6721 ctctttattg attctgcctt atttttgcaa tctatgatcg tgtaaccttc tttgaaattc
     6781 agagccgaac acatcttttc tatcttatcg gccgaaccgt agaagcggaa ggtggcgatc
     6841 ccttctagat aagcttccct cgccgatgag ataaccactt catcttcggc ggcagccacc
     6901 gaaactacct tcggattttt ctttgccagt tccacgagcg ttgaaagatt ggttatcaaa
     6961 gtaccacctc cggatggaac tctctcgctt tttcgacacc ggtgagtaca cgctctgctc
     7021 ccatagccag cgacatgagc tcattctcac cgggttctat gacgacgaga gcgaatctgc
     7081 tcacgtatcc tttgatcatt tctacaaagc tgccgttgta ggccatgccc cctgtgagaa
     7141 ctatcgcgtc tatctgtccc ccggccactg tgcacattcc acctatctcc ttggcgatcc
     7201 cgtaggccat ggcttcgatc acttcgcgcg ccttttcatc gcttctggcc atttccatag
     7261 ctattctcag atcgtcggta cccagatagg cgaccattcc accggctttg gtgtacctgg
     7321 acaacagctc cttctcggat aacccgctgc tgaagcaggc ttccacgacg cttcctaccg
     7381 gcagctctcc cgacctcgtt ggactgaaag ggccttcgtc gttcgcgttg tttacatcga
     7441 ccatctttcc tctcaattga agcccaacgg atattccgcc tccaagatgc accactacca
     7501 ggttgaggtt ctcgagatct ctaccgaact cggccgccac ctgtctcgcc gccgccttca
     7561 tgttaagcgc atgcaggagc gaaaccctgg gtatctgggg aaggcctgag taacgtgcct
     7621 cgggcgaaaa ctcatcaacg gagaccgggt ctgttatgaa gacagggata ctgccacgtg
     7681 acagctccat ccctatcggg gcggccagat ttgcagggtg atccatttta gagctgtgaa
     7741 ccaggtattg gcacatctct tcgttcaccc tgtaggtacc actctccatg ggaggtagta
     7801 tccctcccct gcaggctatc gcatcgaact gctcaagaga gatctctttg acagcgagaa
     7861 aactccgtat ctcatcggct ctcgacgcca ctcccttgtc gtgcggagaa tcttcacgct
     7921 tcacatgctt caaagactcg gcgagcacaa gcgtagagtc ttcgaaaaca cctatcttcg
     7981 tggaagttga tcccgggttt ataaccagaa ctttcatagt ctcactccct caaagaagat
     8041 tccgttcctc gccgtattcc attataacct tcttcaatct tctaactcct tctattatgt
     8101 cgttttttgg agggagacag aaggagagtc gcatcgagtt cttgcaagtg tcatccagtg
     8161 aaaaagcgat tcccggtacg aagagaacgt tctcggattt gcccatttcc agcatttcat
     8221 cggtgttgaa gccttccgga agggtgaacc atatgaagag accgccatcg ggtctggtcc
     8281 agtggaaccc ttccatatcg ccgaagttct tctcgagttc tgccagcatc agatctttct
     8341 tctctttgta aagggctatg gtcggtttga tctgtgccat aatatcgtgt ctctgaaggt
     8401 atctggccgc gagcctttgc gtgagactgg gcgtgcagag atcggccgcc tgtttggcca
     8461 tgaccatctt tctaatgaca tcgtgacttc cgatcaccac tccgagtctc agaccgggag
     8521 agagtatttt actgaaggtt ttgagaagca gaaccctgtc ttttccaccg attttgaata
     8581 cggaatctat ggcttcgcca tcgtacctga gatccccgta aggatcgtct tcgactatca
     8641 ggacatcgta cttttcggct atttcaacca gtcgctttct cttggccaga gacatcgtaa
     8701 cgccgccggg gttgtggaaa ttggatatga cgtatatgaa cttgaccttt ccgatatctc
     8761 ccctgacctt tatctcttcg aggctctgtt cgagttcatc gagatccacg ccgtcttcgg
     8821 tgagattcat gtaaacgtac tgtctggatc tcaaagagaa ggtgctggca gctcccagat
     8881 agactggctt ggaaacgaaa tagatgctgt cctcgtccaa aaaaatctta ctcaccagat
     8941 ccagcgcact ctgagaacct acggtgatga gaaggttgtc cagttccaga ccttcgatcc
     9001 cgttctctct cttgagcagg gagatgtact cctctttgag tatcgggtct ccctcggtag
     9061 atgcgtattg cagtgtatat ctgtattcat tttccagtat ttctctggat atttcggcca
     9121 tttcgtgcct tggaaaggtc tccggatcgg gaactccccc tccaaaggaa atcattccgg
     9181 gtttgccggt gactttgagc aactctctta tgatgttaga cttcatcttt ttggccatct
     9241 tggaaaactt gtcggtcatt gtggaacacc ccctaatttt ttttgttttt tcgatttatg
     9301 tttcaatgtg ttaaaagcaa aaacatgcca gaccattcgc caaatcaggt ctggctttta
     9361 aagcgaatta tagaaaatta aacttcagaa aggcctgggt tgtgcaagta tttgcgtgca
     9421 gtcttgttca acactgcaaa tctctgcact tatgcacagg aagtggatca aaaactgaag
     9481 aaccttccgt atccagcctt aaaattgaat cccagcttct cgccgaacga tagcaaggat
     9541 gagaataaaa actcctgtct gccatcgggg ctgtagtgta gattaaaact cagcaaggcc
     9601 tttctggtga tcggttcggc cagtctgtac cagtcgtatt cgaaaggttc gccgtaataa
     9661 ttgtcgatct tgtaaacgcc cgatctaaga tactccaggc ggatttcggt cagcaccttt
     9721 tccgtttcgt acgagagcgc gagaaggtcc agagtcacgt ccgggccgta agggaatccg
     9781 tagaagccgt tcacgaccac gtactgtcca ccctctatat caccggcgtt gaagcggatc
     9841 ggattggtgt attttccctc ttcgaagtct ctgttgtaga ggtagggagt gctgtggtaa
     9901 tgctcgtact tcaatttcag tcctccggag ttctcgaaaa cagtcttttc gcggagtgtg
     9961 aactgactgt cccatagaag cggaccggag taattcccgc catcgataaa agtgtatgta
    10021 ccgccggcat accaacccag agaagtgggg tttttcgttc tgtctcccgg tataagtata
    10081 tcgtcggcgt acagtaaccc gtacagatcg aaacggtcga gatccagctc caggaaaata
    10141 ccggcgttca catttgagtt cctggcgtaa ttgttgtgga agatggtgaa ggggctgagg
    10201 tctctcaagt cgggaaaggt tttgctgatc aggttggttt cgaccagacc taacctcagg
    10261 agttcgttgc tgaagccgaa cgagtgaccg atcaaagtct taggactgtt atagaccgat
    10321 ctatcggaag agatgaggaa aaagttgtag aaatatctgc cgtaggagac ctctgtgttc
    10381 aaatcgaacc acagatgttc gaagtaagga acggtttccg acaacacgaa gttgtacgtg
    10441 gccggcccga agtggagctt cctcttgcct gccgaaagtc tgatcaggga gttctcgtat
    10501 tcggccagtc cgaccctggg cagatcggtg tcgatataga gagagttctt gtttggatct
    10561 agtggcaggt tggaccagcc tttaccccgc aggtaggccg aaagatcctg cctgaaatcg
    10621 aacctcaaga gcgccctgta agaggggccg gataattcga ttccgaaagt cgcaaaagcc
    10681 gggtatgtga agtagtcgta catatcgtac atagtcggcc ggtagtctct atcgtgcgcc
    10741 ggtagattgt tgagcgaaag caccggaaag aaactgatga agaatcccgg gccggaaaag
    10801 gcgaaaaccg tcactatcaa gaacattaag atcagaaaga gcctcttcat ttcgaactac
    10861 ctcccataaa ttcctgagtt ggattgatcg caattaatta agtcaacttg actcatccat
    10921 taattatcgc ttcgatatcc ctcatcaccc cgtccagatg gccggggtta gtcacaatat
    10981 cgactattct ggcatcgatc ctgtacaccg gagagcggtt gtaactccct atccagtcgg
    11041 agtaaagcct atccagctgt tcccagtacg cgatcggtac ggccagctcc atttcgcgcc
    11101 ctcttctcct gatgcgcgtg agaatcgtgt cgatatcggc gtctatgtaa accagaaggt
    11161 cgggattctt cagatagtcg atcatcgcat agaacatggt tgtataggca tcatattctc
    11221 tccgggacat ctttccggtt tcaaagaggt tgcgggcgaa gatctcggca tcttcataga
    11281 tggagcgatc gaaaaccatg ttttcgttgg cctccgcgtg tttcagtgaa cagtatctgt
    11341 gatagagaaa gtaagtttgc agatggtacg accatctctt ctgatcgtag taaaaatcct
    11401 ccagaaaggg gttgtcggaa acggattcga aatggactcc gtatcgcaat ctctcggcca
    11461 ccgctccggc aatcgtggat tttccggcac cgacattccc agcaaagacg attattcttc
    11521 ccaaagttta tctcctccgg tcatcgcaaa gtggattggc aagacaaaac cggcatgatt
    11581 gccctacaac agattctatc atcaatgaca aacaaagaaa acagcggcag atacaacttc
    11641 catttttgag aatacgggtc ggccggtaga ttgttcagta cgattgaatt gatgatgttc
    11701 aatagatggt cttaacggaa gcgttgttgt ataatctctt taaggaactc cggttgagtt
    11761 cgcacaaaac actgaggagg gatagagatg aagaaactgc ttttgctgtt tgtggttctt
    11821 ggactggccg tggcgctttt ctcagagcct ttgatcgtat ggcccgataa ggctcatgga
    11881 aagcccctgg tggccgggct gcattccccc gtgtacggag aggccaaact ggacgtcttt
    11941 ggaaacataa ccggctggac tgggccgaac cttgggcttg gctggacctg gaaaacctat
    12001 ttcagtcctc tcgaattgca gaaggtcaat ttctactatg aatttggaac caacctcgtg
    12061 atcttcccct acatcggtgc gggattcgat tatgccctgg ttctccagaa caaccagact
    12121 cttctacttg gagcagggat agccgcttca ccgcttcagg ttctgtctgc gattctcgct
    12181 cctacaacca ttcccgcaag cataatctcg gcgataaagt tgagtgtagc cgtcgtattc
    12241 tgaaaaatct gagcgcaacc gatataatag accccgaaag gggtcttttt tgttgagcga
    12301 agatctttct agcaaacaag aacatgatag cgaagtaaga tcgacaatca ccaaatactc
    12361 cataatcctg ggcatagccc tggcgattat acttattctt ctctctacca ggggacgggt
    12421 ggagagcttt gatccggaaa gcacgatcgg tacgatagag cggaccgcgg agctcttcat
    12481 aaaagagaga ttgctggatg agggcgaaag tctggagagg attgagattg tcaaagtcaa
    12541 ttactccacc gttgaatggg gtgagtacac ctacgaagcc accatccagt acagaactct
    12601 ttcgggagag gaaacacttt tcaccggctg gaagtattcg gtgaaaagtg acagtctcga
    12661 tctccttctc tactccaggg gagacggtcc accggtagaa ccgtagcatt ttgaaggcta
    12721 tgggcctgtc aattcgatct tagagatgaa caagagattg acttaaggca tggaatgagt
    12781 gtaaaatcta attatgtttg agatgctcta taaatcctta aaagggggta ttcacatgaa
    12841 aaagctgctt attctggttg ccgtgcttat ggttgcttcg atctttgttg cagctccaaa
    12901 gaacttcatt ctcggtcagg gtctctacgg agatgtcgtg tacgatatga tgggtaatat
    12961 caccggtttc gagggaatgg acttcttcct tttccaggct agcacctatt acgagaaacc
    13021 gataacacca aacgaagtga acgcctttac gttcgacgga aagttcctgt ttttcattcc
    13081 cgtcttcggt agaggctggg aatttctgct gagacctgcg gac
//
LOCUS       DAOOLY010000040        13067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5258_length_13067_cov_6.784430, whole genome shotgun sequence.
ACCESSION   DAOOLY010000040 DAOOLY010000000
VERSION     DAOOLY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_13067_cov_6.784430"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1064)
                     /locus_tag="PLD15_03435"
     CDS             complement(45..1064)
                     /locus_tag="PLD15_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB62984.1"
                     /translation="MITRNEILEQPQKHGILSRRYRSLSRPLVDFIGKSGPASIDFVG
                     CGTSYFLAMGCAKQLERLSKGRIRSSYYSGSEIMLGLAGPSERDLLIGLSRSGTSTET
                     LLALEKAHRDGVRTAALTCQPGSKMIEMADLPVVLDFIEETAIVMTKSFTSMAFFVSA
                     LARELFAPGSLDDYLNNIPASSRATLDDSLNCLETFAIADLDHFVFLGYAEYFAASME
                     GIIKVTEMSLTEADSYQTLEYRHGPKSKVRKGTLAVVSTNGKSLSEELAMAGEIERLG
                     GRPVIISGGKVDGFANIATRYGGEDFGDWFLRAIPLQLLGVERAIARNLDPDRPQNLT
                     KVVQL"
     gene            complement(1321..2505)
                     /locus_tag="PLD15_03440"
     CDS             complement(1321..2505)
                     /locus_tag="PLD15_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPB62985.1"
                     /translation="MSGPLSGIKVLDLTRVLAGPFCTMLLCDLGATVIKVERPVTGDD
                     SRGFGPMVNGESAYFMSINRGKKGITLNLKRARGREILLELAARSDVLVENFRPGTME
                     RLGLGYERFKEKNPVLIYASSSGFGHTGPLKDRTAYDIVIQGMSGMMSITGPDVENQS
                     KVGSSIADILCGIFTALAITAALNNRRETGRGQYIDVAMLDCMISVLENAILRYSVTG
                     EVPRPVGNRHASIAPFGTFECLDGKINIGVGNDQLWKTFCRVLSIEECEKDERFSKNT
                     NRAENVDELTEIINEKLRGKSLDEWLRIFEREGIPSGRICNIADLFENPQVIAREMIV
                     NLPVGERKIGMAGTPLKFSETPANTGGPAPKIGEHNEEILKNLLGMESYEIEKLHQEG
                     II"
     gene            complement(2502..3596)
                     /locus_tag="PLD15_03445"
     CDS             complement(2502..3596)
                     /locus_tag="PLD15_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB62986.1"
                     /translation="MNRSFIELLKRISTIPSPTFHEKEKCELIAGLLEKAGLRVSRDG
                     LYNVLAWSGEELPQSVILIEAHMDTVFDESVPLNWIEEDETIRCPSIADNSVALAALV
                     VLAQKHSNWPDVVFAATTREEGPGKGEGMKRLIEGILGTGREIKYSISLEGIPQGRIT
                     YTGIGSIRAKVSIETTGGHPWLEPSAPSAVHTAAAIVSKIVDSSGYRESPKTTVNVGT
                     ICGGTLPTVRASESYFTLDIRSVDRESLHKTFDLCKRLAGDICAQAKAGIKWEIISSR
                     DPVSLREDHELIGYAREVHRRLGIESQIRPAATNASLPLSMGLAAVTVGVCEGGKTHS
                     REEWISKKGIERGILQLEYLLELLQRGDGA"
     gene            complement(3577..4704)
                     /locus_tag="PLD15_03450"
     CDS             complement(3577..4704)
                     /locus_tag="PLD15_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62987.1"
                     /translation="MKAGFSETRIEPETGLEMAGYIDRLEMSSGIHRPLEMKALFIGD
                     RDPFWLVAVDLLGIPASFRPNDTVAVATHTHAAPEPGLLTDQLNSAFDRCSDRARKVA
                     SHLSGIEHITFEVEGVCSRRTSGEQSSVVGGDILVFECETGRIGIVVFPCHPTVLGPE
                     NTLYSPDLAGGIRQALTRKFGFPFMYFNSCAGDVSTRYTRAGRSSDEIERLSGLFVSG
                     MVETGRKRIVPSGPSYSEHSFEMPVKSPDEKPPYPARPEALPGYRLALKRLESKGLDK
                     YRPARLGLLELGEIAMLFLPFELFLDTGEILKRIARKHFEVPLVVCYALDYLPYVVPA
                     GMEGGYEWYASPYDERAESHLISEVESFLLAGRPKDEQELY"
     gene            complement(4688..5647)
                     /locus_tag="PLD15_03455"
     CDS             complement(4688..5647)
                     /locus_tag="PLD15_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPB62988.1"
                     /translation="MERELFLGIDAGGSQTKLAVCDGRGRLVGYGKGGPANHLSVGLE
                     RMKESLVEAMNDAGCSGLHFESAYIGYSGLGIWSPSELLEKIGHEVIDTDRLAINNDC
                     YNALYGGFGGESGIVAVTGTGSMVLAIDDDGEVTRLGGWGHILGDWGSGFRIAMDAIQ
                     VALKVHDGTVDSLLHDKMMSFFGFDSSRAIVRYFYIEQHSKSEIAAFAPLVIEAAVSD
                     DREARRIVEANAIEIARTAVVMYNKVAVAPRLAVVGGLSKSEFFMEQLRWGVSGWLKL
                     CKPLLQPVFGAVLIAMNNFRKVDKPSLERLSEISGEYEREGRL"
     gene            complement(5651..6499)
                     /locus_tag="PLD15_03460"
     CDS             complement(5651..6499)
                     /locus_tag="PLD15_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB62989.1"
                     /translation="MNKKGKKLIGWSLRYMILIGVSLFTLMPLLWAFSTSITPDTGIV
                     TYKIIPKSVTLDNYPAAWDYPRLFDENVTLGTMFLNSILVAGAITLASLLFDSMAGYA
                     LARKQFFGKTLLFWAALSTLMIPFYVIVIPLYLIILRMGLINSYTSMIIPFMSSGFGV
                     FMFRQTFFSIPLELEEAARMDGAKDFYIYWKVMLPLVKPTIATMAIMKVLWSWNMFFW
                     PLLVVQDYSKMPINLGLTVFRGHNITRWGLMSAGMIIATIPVLILFLSLQKWYIQGLT
                     SGAVKG"
     gene            complement(6512..7402)
                     /locus_tag="PLD15_03465"
     CDS             complement(6512..7402)
                     /locus_tag="PLD15_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB62990.1"
                     /translation="MPKRRLWRDTLVAYLFCAPAYTVFVLFIFIPIIWALFLSFYDFN
                     IMTFLFPDFVGLRNYQKLFTDRYFWLALKNTAVFSLMYVPISMLAGLVFALILHERFP
                     GRNIFRLSIFIPSVISMVVASIVWMLILSPGSSGLANRFLGTFGLESRGWLSESRLAM
                     FSIVIVMVWKDFGYNMLIYLAGLQNIPGELYQAAELDGASRPQRILKITLPLLKPTTF
                     FLSVTTIIGSFQIFTPIYIMTGGGPGYATTTLVNYLYTKGFQEFEMGYATAISFVLFV
                     ILLGLTIVQRKLSRGSDTVM"
     gene            complement(7455..8729)
                     /locus_tag="PLD15_03470"
     CDS             complement(7455..8729)
                     /locus_tag="PLD15_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB62991.1"
                     /translation="MKGKLLVFLAIALLSTVLCAKEITFWFAGGDPTLDLPVVQRQVE
                     EFEKETGIKVKLVVVPWAEDPHTKLQVAIMSGKAPDLVKLGSPFEHVLARFGVLEPLD
                     GLISEELKEQLLPSVLTASLYTSITPKDMTGKLISIPYFTDTRAILYRKDIFQERDVP
                     EPIGWTWSEFVTYAKQLTFDRDGDGKIDVYGFGTAANYTYQAVMYAWQAGGELMGADD
                     RPAFTTDEFNEGIQFYADLFNVHKVVQPGAINVDLWDVRRQLAEGTVAMYIDAGDSAK
                     ELQKILGDTLGVGLLPTNEKTGKFTSYFGSDALVIPKQSRNKSEAAKLLEWLVDKDHM
                     TEYCIASGFLPARLDSAADPHFSDSPVVSMYLRQLEDAHAWIAHPEANFIARTLRVEI
                     QNAISENSDIAAALKTAQELVEKQLKDKGFLK"
     gene            complement(8744..10417)
                     /locus_tag="PLD15_03475"
     CDS             complement(8744..10417)
                     /locus_tag="PLD15_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPB62992.1"
                     /translation="MKRFLILLVLLLLTAGLMAVEFDGATIMKMAINLNTKINDSGRV
                     IPDTIEIPGTDRSVSMEEALYLVAKWMAIYGANGEKIGQLPASVPYIEVKAPQKQLQG
                     EIGGMINWPDIYEVSKQITAKLEVDPLIPSQVEFLLNGESMAVMTPDILLYVLTRSVS
                     WVNDNGSMPNYASLREVSGPANWPPVEKAAPEVIEKIPGSEGEIRAVWAWSTDFISYG
                     VDRAVNELKEAGFTDVFLLVKGTAGQVSWPSAIAYEKIDDTTILEKTVAACREAGLRI
                     HAWFVVNQDQAYLKRFPESKMWGVPIEEGADPQKAGSTVEFVQDTRYREYLVDLMKEV
                     IILYDVDGIHLDYIRYPTGAWGWGPYNIGRAWLEGLDVDFLMETAKETWSSVGDNKKF
                     IDLYRQFTYFDINRWVEMRMDDIRAFTEEIKAGIESMKPEIIYSASLMPEGGDIDAAS
                     NGFAMVHYGQRYADFGELCDIVIPMTYHLDFGEKANWVADVATGTRDVMMSDTKVVMG
                     IQAYDLSPAELQKAIYYARGVGADGIMFFRFGTLFGNENLKNAMLEAFK"
     gene            complement(10436..11167)
                     /locus_tag="PLD15_03480"
     CDS             complement(10436..11167)
                     /locus_tag="PLD15_03480"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB62993.1"
                     /translation="MNLWRQYFSRLEKILETVEKNEDSKIEKAAQLIFEALQRNRLVY
                     VFGTGHSHMLAEEMFYRAGGLVSIYPILETSLMLHESAARSSKLERLPGLARVILERY
                     SLKEGDVMIVASNSGVNAVPVEAAIRGRELGASVICITSIAHSSSVEPRNPAGKRLFE
                     VADVVIDNHIPIGDGLLDISNTKVAPASTAVGATILNSIVGQVAWLYSKRGELPPVFS
                     SANTQNGDLENSELIQIYRKIIPIL"
     gene            complement(11167..>11835)
                     /locus_tag="PLD15_03485"
     CDS             complement(11167..>11835)
                     /locus_tag="PLD15_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HPB62994.1"
                     /translation="DVRTASRVDRGSLYGYLRRVEMNIFLISEEGKSREALMTMFSER
                     GFFIVQALEETTDLGRVDLVVIGAGRATTFGSRFGGGVLKKLTRYVFEGGRYLGICAG
                     AYLALQPYNSETTGFNLSPREALDIGNWDRGCGDVKINWEGRPCVVHYENGPIFGAEP
                     DGCEEVAAVYVDSFDGVMKDGIAAVKGQAGRGRYFLFGFHPELKENTRFMVEEAIDFL
                     FREE"
     assembly_gap    11836..11935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11936..13029)
                     /locus_tag="PLD15_03490"
     CDS             complement(<11936..13029)
                     /locus_tag="PLD15_03490"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPB62995.1"
                     /translation="MNSGIISRFFSFGFGLNEEKGAIGPSLIDLLEEGISAINISARN
                     VVNPPSLWNLIGFIRERSGSIPFIMSDEEGGPVKRLVPGFTRGISHMGLAKGEPSMAY
                     DMASRMAREASAVGVNVFFSPVLDCNSETANPIIGIRSFSADPHETARFGAEFCRGLH
                     SAGVLTTGKHFPGHGATREDSHLCLPVVDISEKIFRSVHLFPFEKLIGEGHLDFLMTA
                     HIVYPSIDDKPATLSRKILTTIAREELGFTGVIITDCLEMDAMKKIYGIERATVDAFN
                     AGADMILLSHTPELQRRAIEALKGAVEDGTIPMRRVKESLLRIEETREKLSHPDYRAG
                     FSQVLFAPDDLLQKTLAEKSVVIGRNGGDIP"
BASE COUNT         2738 a         3789 c         3323 g         3117 t
ORIGIN      
        1 ggtccctttt ttctcaacgc tgatccgcca gctcctcgtt atatctagag ttgaacgact
       61 ttcgtgaggt tctgcggtct atcgggatcg aggttcctgg cgatcgcccg ttcgacgccg
      121 agcaactgga gcggtatagc tctgaggaac cagtcgccga agtcttcgcc gccgtaccgt
      181 gtggctatgt tcgcgaaccc atcgactttc ccgccggata ttatcaccgg tcggccgccg
      241 agcctttcta tctctccggc catcgccagc tcctcgctca gggactttcc atttgtagaa
      301 acgaccgcca gagtgccctt tctgacttta gatttcggtc cgtgtctgta ttctaatgtc
      361 tggtaagagt cggcctcggt gagagacatc tccgtcacct ttattatccc ctccatagag
      421 gcggcgaagt actcggcgta tcccagaaag acgaagtgat ctaggtccgc tatcgcaaaa
      481 gtctccagac agttcagcga gtcgtcaaga gttgcacggg aggacgctgg gatgttgttc
      541 aggtaatcgt caagactccc gggggcgaac agctctctgg caagggcgct cacgaagaag
      601 gccatggacg tgaaggattt cgtcatcact atcgccgtct cttctatgaa gtccagcact
      661 accggaagat cggccatttc gatcattttg cttcccggct ggcaggtgag ggcggccgtc
      721 cgcacgccgt cccggtgggc cttctcgagg gcgagaagcg tttcggtgga agttccggac
      781 ctcgaaagtc ctataagcag atctctctcc gatggtccgg ccagaccgag cattatctcc
      841 gaaccgctgt aataggacga cctgattctc cccttagaga gcctttccag ctgcttcgcg
      901 cagcccatgg cgagaaaata cgacgttccg cagccgacaa aatcgatgct cgccggccca
      961 ctctttccga taaaatcgac cagaggccgc gagagactgc ggtaccttct tgagagaatt
     1021 ccatgctttt gcggctgctc gagtatctca ttcctggtta tcattcgacg cctcctcggt
     1081 atcgtcattc aataacaata ataacccgcg aacgcttatt tcatcataag agccgttgtg
     1141 agtggtgagt aaaaacacga gagaaagagc gtcgagagtt cgcttcgctc aagagaaggc
     1201 gagagcgaaa atagtgactg acgggctcct cgcgtctttc ccatttttcg cgtcctctct
     1261 ctttctctct tctctctctc ctcctctcgc tcttacgaag gagggctcct cacgcacgct
     1321 ctagattatc ccctcctggt gcaacttttc gatttcatag ctctccattc ccagcaagtt
     1381 cttcagtatc tcttcgttgt gttctccgat ctttggggcc ggacctcctg tatttgcagg
     1441 cgtttccgag aatttcaagg gagtgccggc cataccgatc tttctctccc caacaggcag
     1501 gttcactatc atctctctgg cgatcacctg ggggttttcg aagaggtcgg ctatgttgca
     1561 gattcttccg gaggggattc cttccctttc gaatattctt agccactcat ctagggattt
     1621 tccacggagt ttttcgttga ttatctccgt cagttcatcg acgttttcgg ctctgttcgt
     1681 gtttttcgaa aaacgttcat ccttctcgca ctcttctatc gatagcacac ggcagaaggt
     1741 tttccacaac tggtcgttgc caacgcctat gtttatcttt ccgtccagac attcgaaagt
     1801 tccgaagggg gcgatcgatg catgccggtt tcccacgggc cggggaacct cgcccgtcac
     1861 agagtagcgg agaatggcgt tctccagcac cgatatcata cagtcgagca tcgccacatc
     1921 tatatactgc cccctgccgg tttctcgtct gttgttgagg gcggccgtta tggctagggc
     1981 ggtgaagatg ccacagagta tgtcggctat cgagctgccg accttactct ggttttccac
     2041 gtccggaccg gtgatgctca tcatgccgct cattccctga atgactatgt cgtaagccgt
     2101 cctgtccttc aggggtccgg tgtggccgaa gcccgaactg ctggcatata tgagaacggg
     2161 gtttttctct ttgaatctct cgtatcccag accgagtctt tccatagttc ccggcctgaa
     2221 gttctccacc agtacgtcgg acctggcagc cagctccagc agtatctctc tgcccctggc
     2281 ccttttgagg ttgagcgtga tgcctttttt gcccctgttg atactcatga aataggcgct
     2341 ctcgccgttc accatcgggc cgaagcccct cgaatcgtct ccggttacag gtctctccac
     2401 ttttatgacc gtcgcgccaa ggtcgcacag aagcatggtg cagaagggcc ccgcgagaac
     2461 tctcgtgagg tccagtactt ttatacctga cagcggaccg ctcaagcacc atcacctctc
     2521 tgcagcagct ccagaagata ttcgagctgg agtatgcccc tctctattcc tttctttgag
     2581 atccattcct ccctgctgtg agtctttccg ccttcgcaga ccccgacagt tacggccgcc
     2641 agtcccatcg agagcggaag gctggcgttg gtcgcggccg ggcggatctg actttcgatg
     2701 cccagccgac ggtgcacctc cctggcatac ccgatcaatt cgtggtcttc cctgaggctt
     2761 acagggtctc gcgagctgat gatctcccat ttaatcccgg ctttggcctg cgcgcagata
     2821 tctccggcca ggcgcttgca gagatcaaag gttttatgga ggctctccct gtcaaccgat
     2881 cttatatcca gcgtgaaata actctccgag gccctcacgg tcggcaacgt gcctccgcat
     2941 attgttccga cgttcaccgt cgtctttggc gattctctgt aaccgctcga gtcgacgatt
     3001 ttcgagacga tggcggcggc ggtgtgaacg gcgctgggcg cggagggttc cagccagggg
     3061 tggccgccgg tagtttctat ggaaaccttc gctcttatag agccaatccc ggtgtatgtg
     3121 atccttccct gcggtatgcc ttccagcgag atagagtatt tgatctctct tccggtgccg
     3181 agaattccct ctatgagccg cttcataccc tctccctttc ccgggccctc ttccctggtc
     3241 gtggccgcga aaaccacgtc cggccagttc gagtgtttct gcgccagcac aaccagcgcg
     3301 gcgagggcga cggagttatc ggcgattgag gggcatctta ttgtctcatc ctcttctatc
     3361 cagttcagcg ggacggattc atcgaagacg gtgtccatgt gtgcctctat aagtattacc
     3421 gattgcggga gttcctcgcc cgaccaggcc agcacgttgt acagcccgtc gcgggatact
     3481 ctcaggccgg ccttctcgag aaggccggca atcagttcgc acttttcctt ttcgtggaag
     3541 gttggcgaag gaatcgtcga gattctcttc aaaagttcaa taaagctcct gttcatcttt
     3601 tggcctcccg gcaagtaaaa agctctctac ttcacttatc aaatggcttt cggcccgttc
     3661 gtcgtagggc gaggcgtacc attcgtaacc gccttccatc ccggccggta caacgtaggg
     3721 aagataatcc agcgcgtaac agacgaccag cgggacctcg aaatgcttcc tggcgattct
     3781 cttcaggatt tctccggtgt cgagaaagag ctcgaagggg agaaagagca tggcgatctc
     3841 tcccagctcc agaaggccca gtctggccgg tcggtactta tccaatccct tcgactcgag
     3901 ccgcttcagg gccagtctgt aacccgggag cgcttcgggt ctggccggat agggtggttt
     3961 ttcatccggc gatttcactg gcatctcgaa agagtgttcg ctgtatgacg gaccagatgg
     4021 aactatcctc tttctaccgg tttcgaccat gccggacacg aagagaccgg agagtctctc
     4081 tatctcgtca gaagatctgc cggcgcgtgt gtagcgcgtg ctgacatctc ccgcgcagga
     4141 gttgaagtac atgaagggga agccgaactt ccgggtcagc gcctgacgga taccgcccgc
     4201 cagatcgggc gagtagagag tgttttccgg tcccagaacc gtcgggtggc acgggaagac
     4261 cactatcccg atcctccccg tctcgcactc gaaaacaaga atatctcccc ctaccacgct
     4321 agattgctcg ccgcttgtac ggcgcgaaca gacgccctca acctcgaagg ttatgtgttc
     4381 tatgccagag aggtgactgg ccacctttct tgcccggtcg ctgcaacgat cgaaggccga
     4441 attcaactgg tctgtcagca agcccggttc cggcgcggcg tgggtatgtg tggcgacggc
     4501 tacggtatcg ttcggtctga aagaggccgg tataccgaga agatctacgg cgaccagcca
     4561 gaagggatcg cggtctccta tgaataaagc cttcatttcg agcggtctgt gaattccgga
     4621 tgacatctcc agcctatcga tataaccggc catctcgagc ccggtttccg gctcgattct
     4681 cgtctcgcta aagccggcct tcacgttcgt actccccgga tatttcgctg agtctctcga
     4741 gtgaaggttt atctaccttt cggaagttgt tcatggcgat cagtacggct ccaaaaaccg
     4801 gctggagcag gggcttacag agtttcagcc agccgctgac cccccaccgg agttgttcca
     4861 tgaaaaactc cgacttgctc aatccgccga ccacggccag tctcggagcg acggccactt
     4921 tattgtacat tacgaccgcc gttctggcta tttctatagc gtttgcctcg actattctgc
     4981 gggcctcgcg atcgtcggaa acggcggctt caattacaag gggagcgaag gctgcgatct
     5041 cggatttcga gtgctgttct atgtagaagt agcggacgat agccctcgag gaatcgaaac
     5101 cgaagaaact catcattttg tcgtgaagaa gggagtccac tgtcccgtca tgcactttga
     5161 gcgcgacctg tatggcatcc atggctatcc tgaagccgct gccccagtct ccgagtatgt
     5221 ggccccagcc tccgagcctg gttacctcgc catcgtcgtc gatggcgagg accatcgagc
     5281 cggtgccggt aacggccacg atgccggatt cgccgccaaa gccaccgtac agggcgttgt
     5341 agcagtcgtt gtttatcgcc agtctgtcgg tgtctatcac ctcgtggcct atcttttcga
     5401 gaagctccga aggactccag atacccaggc cggagtaacc gatataagcg gactcgaaat
     5461 gcagcccgct acagccggca tcgttcatag cctccacgag cgactctttc atcctttcca
     5521 gtcccaccga gaggtggttc gccgggccac cctttccgta gccgacgagt cttccccggc
     5581 cgtcgcagac cgcgagcttg gtttgcgatc cgccggcgtc gatccccaag aacagctctc
     5641 tctccataaa ctaccccttt acggcgcccg atgtgagtcc ctgtatgtac catttctgga
     5701 gcgacaggaa gaggatcagc accggaatgg tggcgattat catccccgcg ctcatcagtc
     5761 cccagcgcgt tatgttgtgt cccctgaaga ccgtcagccc caggttgatc ggcatcttcg
     5821 agtaatcctg aactaccagc aacggccaga agaacatatt ccacgaccag aggaccttca
     5881 tgatggccat cgtggctatc gtcggcttga ccaggggcag catcactttc cagtatatat
     5941 agaagtcctt cgcaccgtcc atcctggccg cctcctccag ctcgagcgga atactgaaaa
     6001 acgtctgcct gaacatgaag accccgaagc cgctcgacat gaaagggatt atcatgctcg
     6061 tataagagtt tatcagcccc atcctgagta ttataaggta gagggggatt actattacgt
     6121 aaaaggggat catgagtgtc gagagcgcgg cccagaagag cagagtttta ccgaagaact
     6181 gctttctggc cagcgcgtag ccggccattg aatcgaagag aaggcttgcc agggtgatcg
     6241 ctccggccac caggatactg tttaggaaca tcgtcccgag cgtgacgttc tcgtcgaaca
     6301 gtctcggata atcccatgcg gccgggtagt tatccagcgt cacggatttc ggaattatct
     6361 tgtaagtcac tatgcctgta tcgggagtga tggaagtgga aaaagcccag agaagtggca
     6421 tgagcgtgaa gagagagacg cctataagga tcatgtatct caacgaccac ccgatgagct
     6481 ttttgccttt tttattcatc tcaatccccc ctcacatgac cgtgtcggag ccgcgggaaa
     6541 gctttctctg gacaatcgtc agtcccagca gtataacgaa gagaacgaag ctgatcgccg
     6601 tcgcgtaacc catctcgaac tcctgaaacc ctttggtgta tagatagttg accagcgtgg
     6661 tagtcgcgta tccgggaccg cccccggtca ttatataaat cggcgtgaat atctggaacg
     6721 atccgattat cgttgtcaca ctcaagaaga aggtggtcgg tttcaaaaga ggcagagtta
     6781 tcttcagtat cctctgcggc ctcgaagcgc cgtcgagttc tgcggcctgg taaagctccc
     6841 cgggaatgtt ttgaagaccg gccagataga tcagcatgtt gtagccgaag tccttccaga
     6901 ccatcacgat aacgatcgaa aacatcgcca accgcgattc ggaaagccag cccctcgact
     6961 cgagcccgaa ggtccccaga aacctgttgg ccaatcccga agagcccggt gaaaggatca
     7021 acatccagac tatgctggca acgaccatgg agatcacgct gggaatgaat atcgagagac
     7081 ggaagatatt cctcccgggg aatctctcgt ggagtatcag cgcaaagacg agaccggcca
     7141 gcatgcttat gggaacgtac ataagagaga agacggccgt gttcttcagc gccagccaga
     7201 agtagcgatc ggtgaatagt ttctggtaat tcctcaatcc cacgaaatcg gggaagagga
     7261 acgtcattat gttgaaatcg tagaagctca aaaagagagc ccagattatc ggtatgaata
     7321 tgaaaagtac gaagactgta taagccggcg cacagaagag atatgccacc aaagtatcgc
     7381 gccagagcct gcgtttgggc atctcaaaac ctcctcggag aaaataaagg agagagcaaa
     7441 gctctctcct ttcctcattt caaaaagccc ttgtctttca gctgtttctc gacgagttcc
     7501 tgcgccgtct tcaatgccgc tgcgatatcg gagttttcgg aaatggcgtt ctgaatctcg
     7561 actctcagcg ttctggcgat gaagttggcc tccgggtggg ctatccatgc gtgcgcatct
     7621 tcgagttgcc tgagatacat ggagacgacg ggactatccg agaagtgcgg atcggccgcc
     7681 gagtcgagtc tggcaggcaa aaagcccgag gcgatgcagt attcggtcat gtggtcctta
     7741 tccaccagcc actcgagcaa cttggcggct tcggacttgt tcctggattg tttcggtatc
     7801 accagggcgt ccgagccaaa gtaggatgtg aatttcccgg ttttctcgtt cgtgggcagc
     7861 agtcccaccc cgagggtgtc tccgaggatc ttctggagtt ctttggcgct gtcgcccgcg
     7921 tctatgtaca tggcgaccgt accctcggcc agctgccgcc ttacatccca gagatccacg
     7981 tttatcgcgc ccggctgaac gactttgtgc acattgaaga gatcggcgta gaactggatg
     8041 ccttcgttga attcatcggt tgtgaaggcc ggtctgtcgt cggcccccat gagctcccct
     8101 ccagcctgcc aggcgtacat gaccgcctgg taagtgtagt tggccgccgt tccgaagccg
     8161 taaacatcta tctttccatc gccgtccctg tcgaaggtca gctgtttggc gtacgtgacg
     8221 aactcgctcc aggtccagcc tatcggttcc ggcacgtcgc gctcctggaa gatgtctttt
     8281 ctgtacagga tcgccctggt atcggtgaaa tacgggatag atatcagctt gcccgtcatg
     8341 tctttcggcg ttatcgatgt gtaaagactg gcagtcagta cggaaggaag aagctgctcc
     8401 ttcagttcct ccgagatgag gccgtcgagc ggttcaagca caccgaaacg cgccaggaca
     8461 tgctcgaaag gagacccgag cttcaccaga tcgggcgcct ttcccgacat tatggcaacc
     8521 tgcagtttcg tgtgtggatc ctcggcccac ggcacgacaa cgagcttgac ctttatgccg
     8581 gtctcttttt caaattcctc gacctgtctc tgcaccacgg gaagatcgag cgtgggatcg
     8641 ccgccggcga accagaaagt gatttccttc gcgcagagca ccgtcgaaag aagcgcgatc
     8701 gcgagaaaaa caagcaattt acctttcatc gtctctcccc ctttcactta aaagcttcca
     8761 gcatggcgtt cttgagattt tcgttgccga acagcgttcc gaacctgaaa aacatgattc
     8821 cgtcggcccc cacacctctg gcatagtata tagccttctg caattcggcc ggcgagagat
     8881 cgtaagcctg aatgcccatc acgactttcg tatcggacat catcacgtcc ctggttccgg
     8941 tcgccacgtc ggcgacccag tttgccttct ctccgaaatc tagatggtac gtcatcggga
     9001 taactatatc gcatagttct ccgaaatcag cgtacctctg accgtagtgt accatcgcga
     9061 atccgttgga ggcggcgtct atgtctccac cttcaggcat aagtgaggcg ctgtagatta
     9121 tctccggttt catggactct attccggcct tgatctcttc tgtgaaggcc cttatatcgt
     9181 ccattctcat ctctacccag cggttgatat cgaagtacgt gaactgcctg tacagatcga
     9241 tgaatttctt gttgtcgccg acggagctcc aggtctcttt agccgtctcc atgaggaaat
     9301 ccacgtcgag tccctcgagc caggccctgc ctatgttgta agggccccag ccccaggccc
     9361 cggttggata ccttatatag tccagatgga tcccatcgac gtcgtacagt attattacct
     9421 ctttcattag atcgaccagg tactctctgt aacgggtgtc ctgaacgaac tccaccgtag
     9481 agcctgcctt ctgcggatct gcgccttctt cgattggaac gccccacatc ttggattcgg
     9541 ggaacctctt gagatatgcc tggtcctggt tcactacgaa ccacgcgtgt attctgagcc
     9601 cggcctctct gcaggccgcc acggtctttt cgagtatcgt cgtatcgtcg atcttctcgt
     9661 acgctatcgc cgaaggccag gagacctgcc ccgccgtccc cttcacaagc aggaatacat
     9721 ccgtgaagcc cgcctctttc aattcgttga cggccctgtc cacgccgtaa gatatgaaat
     9781 cggtcgacca ggcccatacc gccctgatct ctccttcgct gcccgggatc ttctcgataa
     9841 cttcgggagc ggccttctct accggcggcc agttggcggg tccgctaact tctctaagcg
     9901 aggcgtagtt gggcatgctg ccgttgtcgt tcacccagct caccgatctc gtcagtacgt
     9961 acagcagaat atccggagtc atgacggcca tactctcgcc gttcagcagg aactccacct
    10021 gggacggaat gagcgggtcg acctccagct tcgccgtgat ttgtttggaa acctcgtaaa
    10081 tatccggcca gtttatcatg ccgcctattt cgccctggag ctgcttctgt ggcgccttca
    10141 cctcgatgta cggcacagat gcaggtagct gtccaatctt ctctccgttg gcaccgtata
    10201 tcgccatcca tttggccacc agatacagcg cctcttccat cgagacgctc ctgtcggttc
    10261 ccggaatttc gatggtatcg ggaatcacgc ggcccgagtc gttgatcttc gtgttcagat
    10321 tgatcgccat tttcatgata gtcgcgccgt cgaactcgac cgccatcaat cctgctgtca
    10381 acaggagaag aactagaagg atcaaaaacc ttttcataca aacacctccc tgtgtttaca
    10441 agatcggaat tatcttccgg tatatctgta tcagctcgga attctccaga tcgccgttct
    10501 gcgtgttggc actgctgaaa accggtggaa gctcccccct tttcgaatac agccacgcca
    10561 cctgtccgac gatcgaattg agtatggtcg cccccacggc cgtcgaggcc ggcgcaactt
    10621 ttgtgttcga gatatccaga agaccgtccc ctattggaat atggttatct ataactacat
    10681 cggcaacctc gaaaagcctc tttccggcgg ggtttctggg ttccaccgag gaagagtggg
    10741 ctatggaggt tatgcagatc acgctggcgc cgagttctct gcccctaatt gcggcctcga
    10801 ccgggacggc gttgaccccg gagttggaag cgacgatcat cacgtcgccc tccttcagag
    10861 agtaacgctc cagtatcacc cgggccaggc cgggaagcct ttcgagcttg ctggaacgcg
    10921 cggcgctttc gtgaagcatg agagaagtct cgagaatcgg gtaaatcgac acgagacctc
    10981 cggcccggta aaacatctcc tcggcgagca tatgggaatg gccggtgccg aacacataaa
    11041 ccagccggtt cctttgaagc gcttcgaata tgagctgtgc cgccttctca attttcgaat
    11101 cttcattctt ttccaccgtt tccagaatct tctccagtct ggaaaaatac tgcctccaca
    11161 aattcatcac tcctcccgga aaaggaaatc gatcgcttct tctaccatga accgggtgtt
    11221 ttccttcaat tctggatgaa acccgaaaag aaagtacctg cctctgccgg cctgtccctt
    11281 caccgcggcg attccatctt tcataacccc gtcgaaacta tcgacgtaga ccgcagccac
    11341 ttcctcgcag ccgtcgggtt cggcgccgaa gatcggtccg ttttcgtagt gaaccacaca
    11401 gggccggccc tcccagttga ttttcacgtc gccgcagccc ctatcccagt tacctatatc
    11461 cagcgcctct ctgggggaga gattgaaacc ggttgtttcg ctgttgtagg gctggagggc
    11521 cagatacgct ccggcgcaga tccccagata cctcccgcct tcgaagacgt atcgggtcaa
    11581 ttttttcaac acaccgccac cgaaccgcga accgaaagtc gtcgccctgc cggcgcctat
    11641 aaccaccaga tcgactcgac ccagatcggt agtttcctcg agcgcttgca cgatgaagaa
    11701 accgcgctcg ctgaacattg tcatcagagc ttccctggac ttcccctcct cggaaatcag
    11761 aaaaatgttc atttcaactc tccgcagata gccatacagg ctgcctcgat cgacccggga
    11821 agcagtccga acgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggtat
    11941 gtccccgccg tttcttccga tcaccacgct cttttcggca agggtcttct gcaagagatc
    12001 gtcgggggcg aacagtacct gtgaaaagcc ggcgcgatag tcgggatgtg agagcttctc
    12061 cctggtctct tcaattctca agagactttc cttcacgcgc cgcatcggta tggttccatc
    12121 ctccacagct cccttcaaag cctcgatcgc cctcctttga agttcgggag tgtggctgag
    12181 aagaatcata tctgcgccgg cgttgaaggc atcgaccgta gcccgttcta ttccgtagat
    12241 cttcttcatc gcatccattt ccagacaatc ggttatgatg acgccagtga accccagctc
    12301 ctccctcgca atggtggtga gaatctttct ggaaagcgtc gccggcttat cgtcgatcga
    12361 aggatagact atgtgcgccg tcataaggaa atccagatga ccctccccga tcagtttttc
    12421 gaaggggaag agatgtacac tgcggaagat tttttcggaa atatccacca caggcagaca
    12481 gagatgggag tcctcccggg tggctccgtg gccggggaaa tgcttgcccg tagtcagaac
    12541 gccggccgaa tggagacccc tgcagaactc ggcgccgaag cgggccgtct cgtgaggatc
    12601 ggcagaaaaa gacctgattc cgattatcgg attggctgtc tcgctgttac agtcgaggac
    12661 ggggctgaag aacacgttca ctccgactgc cgacgcttcc cgggccatgc ggctcgccat
    12721 gtcgtaagcc atagatggct cgcccttcgc cagacccata tgacttatcc ctctggtgaa
    12781 acccggtacc agtctcttca ccggtccgcc ctcttcgtcg gacatgatga aagggataga
    12841 tcccgatcgc tcccttataa aaccgatcag attccataga ctcggcgggt tcacgacgtt
    12901 cctggccgaa atgttaatgg ccgatatacc ctcctcgagc agatctatga gcgaaggacc
    12961 tattgctcct ttttcttcgt ttaaaccgaa accgaaagaa aagaaacggc ttattatccc
    13021 tgaattcata ctttttataa acaccactcc aatggacaaa ggatttg
//
LOCUS       DAOOLY010000041        12971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5299_length_12971_cov_5.279421, whole genome shotgun sequence.
ACCESSION   DAOOLY010000041 DAOOLY010000000
VERSION     DAOOLY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5299_length_12971_cov_5.279421"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLD15_03495"
     CDS             complement(<1..696)
                     /locus_tag="PLD15_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPB62996.1"
                     /translation="MMPKDIRKLLQAIEEEITSRTEMNLKDMLNSPSSYVRARAIKSA
                     SDREIIIEDLEAFLEDPSPEVRRSVITSMSRSGIGQDKILLSLEDPSDTVRSAAVKEL
                     IEFGYEDGDLARRVASDPSKKVRKTFVLSLLESGETELLKEFDNDPSNDIQIILSAYR
                     GELELDEERLSALPKRLQKMAFASRMTQRNAQAMSALLESLSKYRSASIKESIIDLIS
                     AFPEEIAVPALKKL"
     gene            complement(693..1115)
                     /locus_tag="PLD15_03500"
     CDS             complement(693..1115)
                     /locus_tag="PLD15_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB62997.1"
                     /translation="MKKGTSLVELSISLAVSALLLISMLKVFALVNSFAFRIAKEAGD
                     SLAIFRTLDMLERDLKLATGNFSCSVDFVSFDAVVDGKTRRIRYYVSKQRIMRRSGNS
                     YNEVMEFGQSAFFTGEGDSVRLTIDGRELLFRVEGDFL"
     gene            complement(1090..2514)
                     /gene="gltX"
                     /locus_tag="PLD15_03505"
     CDS             complement(1090..2514)
                     /gene="gltX"
                     /locus_tag="PLD15_03505"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB62998.1"
                     /translation="MVRCRFAPSPTGNLHVGGVRTALFNWLFAKNQSGNFVLRIEDTD
                     TERSEKKFEDQIFASMKWCGLDWSEGPDIGGPYGPYRQSERVELGIYDRFARELVEKG
                     AAYYAVYHRDDPENVLRTSNERPTDLSADETCTIKFKIPAGVTEFFDLSKGDMSFENE
                     NFGDFVIVKSNGFPTYNFAVVVDDHLMEITHVFRGEDHLTNTPRQIMMYSALGWEHPV
                     FMHIPLILGSDRAPLSKRHGHTSVDHFRSEGYLSVGLMNYLALLGWTVDQEIFDYHEK
                     VKDFVPSDISNKGVVFDYEKLEWINGKHMRLMEPERLLAEFGLWLKYTGRFDYYASVE
                     SEPFYALEVISMCREKINTIEQLYDFSIPFFSEEIDYQEEYVVKYLENGWSKDLIAAA
                     IRRFEGADDWSVEGVERVIRDLAEEKITSKKNTFQLLRGGVTGRLVTPGLFETISILG
                     RDRVLDRLENLLELVEYEEGDFSS"
     gene            complement(2501..3028)
                     /locus_tag="PLD15_03510"
     CDS             complement(2501..3028)
                     /locus_tag="PLD15_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HPB62999.1"
                     /translation="MLQVAVIGYSGPIDRSPVLELTQICRQVGKALAERGDVLITGGR
                     DGVMELVSESASSNGGRVLGILPSNDEGNDHNQIKIRTGMDFALRSLIISKSADVVIS
                     IGGEAGTLLEILSSYSYGRPVILMKGTGGWTDRILPVMIEGRYLDNRRTVEIRQAQDM
                     AQLLECLEEAENGKV"
     gene            complement(3028..3750)
                     /gene="lptB"
                     /locus_tag="PLD15_03515"
     CDS             complement(3028..3750)
                     /gene="lptB"
                     /locus_tag="PLD15_03515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPB63000.1"
                     /translation="MNKVSYLRCLNLEKRYGRRKVLNDVSIEASSLEVVGILGPNGAG
                     KTTAFKSILGIVIPNSGDIYLNEEKITHLPIHERARRGIAYLPQETSIFRGLKVIENL
                     KMVLKLTGERKNNEETATRLLEEFNILPLKDQPSSVISGGEKRRLEFARTLTLSPSFI
                     LLDEPFVGIDPMTVKDIQKMIRKLKDKGIGVIVTDHDVTSISRVVDRLYVLYKGEVIS
                     SGDPSVVLADSVVVEKYLGSDD"
     gene            complement(3747..4244)
                     /locus_tag="PLD15_03520"
     CDS             complement(3747..4244)
                     /locus_tag="PLD15_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPB63001.1"
                     /translation="MKKTLQVILMVVMIAAVGVVVVSEGGAGPSDPAKIAFADMQKVL
                     EATKDWNTLNTDYQKDMQFYQGQLDSLSKEYQDLAASGASQESLAVKQQEILTKKTQY
                     EQTLEATYNAKLQVIMEQVNKRIKDYSTFIGIDMVISSEVVVYGAPAYDVTDAIIEYM
                     KGFQK"
     gene            complement(4256..4702)
                     /gene="dtd"
                     /locus_tag="PLD15_03525"
     CDS             complement(4256..4702)
                     /gene="dtd"
                     /locus_tag="PLD15_03525"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPB63002.1"
                     /translation="MRAVIQRVSRASVRVDGRTTGSIDKGLLALIGVGTSDGIEDLEW
                     LVDKIVNLRVFEDSNDKMNLSLLDVGGSLLAISQFTIMGDARKGRRPSFTDAADPEKA
                     KGLFNDFLKLASKFVRVETGVFQAHMEVELVNEGPVTILLDSNKKF"
     gene            complement(4699..6873)
                     /locus_tag="PLD15_03530"
     CDS             complement(4699..6873)
                     /locus_tag="PLD15_03530"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPB63003.1"
                     /translation="MARTELDAQILLGELESILQYRLKRRDKERILDAYEFARFHHQE
                     QMRDSGEPFISHPVEVSKILAQLSADTDTIVAGFLHDIVEDCNVPLGEITEKFGETVS
                     LIVDGVTKISNLKLNEKLDSKIVKSRMKVETLRKMLLALSKDPRIIIVKLADRLHNMR
                     TLEFLKSSEKREDKARETIQIYAPIAHRIGIHKIQAELEDLSFKYEYPEEFRELKAKV
                     NKKLKERQEIMDEYKNIVLRELRKNRIAAIIEGRVKHLYSIWQKMIKKDRSFDEIFDL
                     IALRIVTGDEVNCYKVLGAVHSLWPPMPGRFKDYIAAPKSNGYKSLHTTLITHRGEPL
                     EIQIRSERMHKEAEYGVASHWVYKEGIDVKDRAWFSQLVDWQKDFIESFKDMESISRE
                     LEVEEVYVFTPKGEVVHLPKGSTPIDFAYAIHTEIGHHYAGAKVNDRLVPVNYELQLG
                     DRVEVVVNKASEGPSLDWLKYVRANSTKAKIKRFFKNEYSVKLIERGKEIFRKISKRL
                     AVSIDDLIEGEEVKNLMVRFAAHNENDLFSKLGDGSITMGEVLSALVPKEEVEVLPEE
                     TELVKQKQAKGNEVVVGGETGIAVYFAKCCTPLPGDDIVAVMSSRGISIHNRGCRNVK
                     GISQDKLVEAHWTMITGEKFSAWIVVEFDSSDKAMANRFLERLESKSAKVKKYSVETG
                     KWGYDTLMANILVKDVAQLTSVMENLRGMKGVQNVKRIGGVM"
     gene            complement(7083..8033)
                     /locus_tag="PLD15_03535"
     CDS             complement(7083..8033)
                     /locus_tag="PLD15_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPB63004.1"
                     /translation="MRYKMLKTIVIAATLIAMAFYLVAVSQKGVDETFWLNHDGIDRK
                     TVVHLPPKGEDDSKLPLVFVFHGLLGNSNYTKNTYGITEISDREGFIAAYPEGTGPLK
                     SVFLSWNGDFCCAYAKENEIDDARYIVELLKELKREYPVDESRVYLVGLSNGGMLTYK
                     LVSDYPELFAAAAIVSSSPAGGASEDEVHRIEPPPRPVPMIIFHGMLDPIIPYHGGYS
                     SASEETVFFPSIPESIDLWIAGMKAVELEETSLESGKVLLKKYGRPGEDFEIHFYTIV
                     DGDHTWPGREKGLDALQSSSRSSVKASELIWDFFRDRRLE"
     gene            complement(8043..8477)
                     /locus_tag="PLD15_03540"
     CDS             complement(8043..8477)
                     /locus_tag="PLD15_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB63005.1"
                     /translation="MASVKEKLTTIVERLSTLMAEQEERALNSGESSRLTVRQMYYLN
                     VIAKMDRPTVSQIADLLSVTKPTVTITVRGLIEEGYLKRIQSEEDRRVFWLELTNRGQ
                     KIIQACRKIHRDFSHRILEILDTREAQELTRIFEKILRKIES"
     gene            complement(8631..9410)
                     /locus_tag="PLD15_03545"
     CDS             complement(8631..9410)
                     /locus_tag="PLD15_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HPB63006.1"
                     /translation="MNTFKADLLGKRIENLRVSGGMTQEQLAEALGKSVPLISLYESG
                     KRLPPTDMIYRIAKYFEVSADYLLGLPDRYTFEYETKTREKLSSTMLDVLIPKHADPK
                     SAEKPVPEKLYTPGVSIYGSIEPSSPQPVHKGVIRRVIPVFTLERTDRLDESSAISYV
                     SDLTGGADLVVQFTDNSMFPTFRKGDSLFIKQNVLPPVGSYVFFRYREESYVRFLSSR
                     PNRGAFEFKPSNPEFETVVIANREPQFTILGIVVGRFTGTA"
     gene            9628..9873
                     /locus_tag="PLD15_03550"
     CDS             9628..9873
                     /locus_tag="PLD15_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63007.1"
                     /translation="MAEEKKNGSPSRDPSKDRYYFRQNDYTTREGKRPATKHSDKIPS
                     REDYRLYHAQLREIYDSCKSCDEAFTEGRELIDRLEI"
     gene            9854..10609
                     /locus_tag="PLD15_03555"
     CDS             9854..10609
                     /locus_tag="PLD15_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB63008.1"
                     /translation="MTDSKSRPASHKSILRVKKTNNYVVLDKGFLQDPSLSCKAKGLL
                     SYLLSKPDDWEIRLSELCRHFSDGKDSIRKGIEELEKAGYIKKERLRGGNGKFAGVRY
                     MVYETPCLSQKEPQTEKPDTVFPAPENPTLLNNDLTKDTSYRVATNFEVSFMSKRECI
                     KKTARLIEDLGVENSYKVAAAMFRNADREIDWHFPIWILENLNEAIERADDKVKYLLS
                     LLQDDNLWHCYASQLEDEAFDERRVLANVAAGY"
     gene            10539..11846
                     /locus_tag="PLD15_03560"
     CDS             10539..11846
                     /locus_tag="PLD15_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPB63009.1"
                     /translation="MRASWKMKLSMKGECLPMLQLGIEGERTLLKALLEYEELRCRID
                     EIDPSYLSEESRKIFEVISDNREMDSTRLCVQIYNKIGNQKFYDGDFITEVGANYLID
                     DYKELVGKNGLQRLCLEISKKLKKGDMKVDEAMEKFYAFQKESSSAFNYISIKEANEL
                     QKKAHTDIWNNRGNRLHWPFKFFEDILNGLRGKELVVVAGETGTGKTAFMLNAAALWA
                     SFGKTVGYISLEIDVVDLKDRMHQKDFAVNLSAESEKLDGQERERLLREIDKTGEQRL
                     FFCDSGVRSVHSIIAGIKLMHHIHRFDVVFIDYLQLMTCPGKQTKDMEIGEIMGSLKA
                     LAMEIRVPIIVGSQLNREVGKNDRGIPRLSNLRESGTIEHSADVVILLYRPILYDRNA
                     SPDEYQAIVAKQRSGRTGTLYMHFDMKRQIMQGVVPGERAYAR"
     gene            11960..12754
                     /locus_tag="PLD15_03565"
     CDS             11960..12754
                     /locus_tag="PLD15_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63010.1"
                     /translation="MRLNENLFERRLGLFFMVLWFVVMFFYITWDGWLYEWRDCGYEG
                     PNQHFDLDMSDMDYEYTADGVKIYHSTNNLHNLIYLCYFVFFFGWLGLLFDIEWAKQW
                     TMATTGISVVAALSTLRLSDHVYILQIVYDAVHLSGIVMGCYLFWKYDLKIRRAVHAV
                     LFTWAVYLSSHLIFTPWPYWENQGKAYFSINQINDLPFFIFGLEYLLVVALLLGIDVA
                     ASFINSRLDNRFLKMAVPLGLYLSLCIILIALGLIIVPEIHINTCP"
     gene            complement(12757..12924)
                     /locus_tag="PLD15_03570"
     CDS             complement(12757..12924)
                     /locus_tag="PLD15_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63011.1"
                     /translation="MKRWKVWEGYEYILLSPDESKRRERAIKYFDSKGKLVAVQILRK
                     SGDLGQLKFPI"
BASE COUNT         2867 a         3543 c         3326 g         3235 t
ORIGIN      
        1 cagctttttc agtgccggaa cggctatctc ctcgggaaag gccgatatca ggtcgatgat
       61 gctctccttt atcgaagccg aacgatactt cgaaaggctt tccagaagag cgctcatggc
      121 ctgcgcgttt cgctgggtca tcctcgaggc gaaggccatc ttctggagtc ttttggggag
      181 ggccgagagt ctctcttcgt cgagttccag ctcgccccgg taggccgaga gtatgatctg
      241 aatgtcgttg ctgggatcgt tgtcgaattc cttcagaagc tccgtctctc ccgattcgag
      301 cagggagagg acgaaggtct tcctgacttt tttcgacggg tcggaagcga ccctccgggc
      361 caggtcaccg tcctcgtaac cgaactcgat caattccttg actgcggcgc ttctcacggt
      421 gtcggaagga tcttcaagag agaggagtat cttgtcctgt ccgataccgc tcctggacat
      481 cgaggttatc acgcttctcc gcacttcggg cgagggatcc tccagaaagg cttcgaggtc
      541 ctcgataatt atctccctgt cggaggccga ttttatcgcg cgcgccctga cgtaggacga
      601 cggcgaattt agcatgtctt tcaagttcat ctcggttctg gaggttatct cctcctctat
      661 cgcctgaaga agctttctta tatctttcgg catcataaaa aatcaccttc cactctgaaa
      721 agtagctccc gaccatctat cgtgagcctt accgagtcgc cctcgcccgt aaaaaaggcg
      781 ctctggccga actccatgac ctcgttgtag gaattgcccg atcttctcat gattctctgc
      841 ttacttacgt agtatcttat ccttctggtc tttccatcga ccacggcgtc gaaagacacg
      901 aaatcgaccg aacaggagaa gttgcccgtc gcgagtttca gatctctctc gagcatgtcc
      961 agcgtcctga agatcgccag agagtctccg gcttccttcg cgatgcgaaa cgcgaaggaa
     1021 ttgacgaggg cgaagacctt cagcatagat atcagcagaa gagcggaaac cgcgagagag
     1081 atcgagagct caactagaga agtccccttc ttcatattcg acaagctcca gaaggttctc
     1141 cagacgatcc agaaccctgt cccttccgag tatggatata gtctcgaaaa ggcccggcgt
     1201 gacgagtctt ccggtgactc cgcccctcaa gagctggaac gtgtttttct tggaagttat
     1261 cttttcctcg gcaagatccc tgataactct ttcgactccc tcgaccgacc agtcgtcggc
     1321 gccctcgaat ctcctgatgg cggcggcgat cagatccttg ctccagccgt tctcgaggta
     1381 tttgaccacg tattcttcct ggtaatctat ctcttcgctg aagaagggta tggagaaatc
     1441 gtagagctgc tcgatcgtgt tgatcttctc ccggcacatc gatatgactt ccagcgcgta
     1501 gaagggttcg ctctcgaccg aggcgtagta gtcgaagcgg ccagtgtatt tcagccacag
     1561 cccgaattcg gccaggagtc tttccggttc catcaggcgc atatgcttcc cgtttatcca
     1621 ctcgagcttt tcgtaatcga agacgacgcc cttgttggat atgtcggagg gcacgaagtc
     1681 cttcaccttt tcgtggtagt cgaagatctc ctgatcgacc gtccagccga gaagggccag
     1741 gtaattcatg aggccgacgc tgagatagcc ctcgctcctg aagtgatcca cgctggtgtg
     1801 gccgtgtctt ttggaaaggg gcgcgcggtc gctgcccagg atcagcggaa tgtgcatgaa
     1861 gacgggatgt tcccagccca gagcgctgta catcattatc tggcgcggcg tgttcgtgag
     1921 gtgatcctct cccctgaaga catgggtgat ctccatgaga tgatcgtcga cgaccacggc
     1981 gaagttgtag gtcgggaagc cgttagactt gactatgacg aagtccccga agttctcgtt
     2041 ctcgaagctc atgtcgcctt tcgagagatc gaagaattcg gtgacgcccg cgggtatttt
     2101 gaattttatc gtgcaggtct cgtcggccga aagatcggtc ggtctttcgt tagaagttct
     2161 gaggacgttt tccggatcgt ccctgtggta aacggcgtag taggccgctc ccttctccac
     2221 cagttcgcgg gcgaacctgt cgtatatgcc cagttccacc ctctcgcttt gacggtacgg
     2281 accgtacgga ccgcctatgt cgggaccttc cgaccagtcg agcccgcacc acttcatgga
     2341 ggcgaagatc tgatcctcga acttcttttc ggagcgctcc gtatcggtgt cctctattct
     2401 caaaacaaag tttccagact gatttttcgc gaaaagccag ttgaaaagcg ccgtcctcac
     2461 tccaccgacg tggagattgc ccgtcgggct gggcgcaaac ctacacctta ccattttctg
     2521 cctcctccag acactccagc aattgggcca tgtcttgtgc ctgtctgatt tccactgttc
     2581 tgcgattgtc caggtatcta ccctcgatca taaccggaag tatcctgtct gtccaccctc
     2641 cggtcccttt catgagtata acgggtctcc cgtaggagta agaggagagt atttcgagca
     2701 acgtgccggc ctctccgccg atcgagatca cgacatcggc cgatttcgat atgatcagcg
     2761 atctgagggc gaaatccatg ccggttctga tctttatctg attgtgatcg tttccctcgt
     2821 cgttggaggg caggatgccg agcacccttc cgccgttcga agaggcgctc tccgaaacga
     2881 gctccatcac accgtctctc ccgccggtga tgaggacgtc tcccctttcg gccagcgctt
     2941 tcccaacctg ccggcatatc tgcgtgagct ccaataccgg ggatctgtcg atcggaccgg
     3001 aatagccgat caccgcgacc tgcagcatca atcatcactc cccagatact tctccaccac
     3061 aacgctgtcg gccagcacga ccgagggatc tcccgaggag atcacctctc ccttgtacag
     3121 aacgtataat ctatcgacca cacggcttat ggaggtgacg tcgtgatcgg tcacgataac
     3181 tccgatgcct ttgtccttca acttcctgat catcttctgg atatctttca ccgtcatggg
     3241 atctattccc acgaagggtt cgtccagcag tatgaaggag ggcgagaggg taagcgttct
     3301 ggcaaactcc agtctcctct tttcgccgcc ggagatcacc gaggagggct ggtctttgag
     3361 cgggagaatg ttgaactcct cgagaagcct ggtggcggtc tcttcgttgt ttttcctctc
     3421 tccggtcagc ttcaaaacca tcttgagatt ctcgataacc ttcaaaccgc gaaatataga
     3481 cgtttcctgc ggcagatagg ctatccccct gcgcgccctt tcgtggatcg gcaggtgcgt
     3541 tatcttctcc tcgttgagat agatgtcccc ggagttggga atcactatgc ccagtatcga
     3601 cttgaaggcg gtggtctttc cggcgccgtt ggggccgaga attcccacca cctcaaggga
     3661 agatgcctct atcgagacgt cgttcaagac ctttcttctt ccataacgct tctcgaggtt
     3721 gaggcatctg aggtatgaaa ccttgttcac ttctggaatc ccttcatgta ttcgatgatg
     3781 gcgtcggtga catcgtaagc cggcgcgcca tagactacga cctcgctgga tattaccatg
     3841 tcgattccta taaaggtgga gtaatccttt attctcttgt taacctgttc catgatcacc
     3901 tgcagtttcg cgttgtaggt ggcctcgaga gtctgctcgt actgtgtctt cttcgtcagt
     3961 atctcctgct gcttaacggc cagcgactcc tggctggctc cggaagccgc aagatcctga
     4021 tactctttgc tcagactgtc cagctgtccc tggtaaaact gcatatcctt ctggtaatct
     4081 gtgttgagcg tattccagtc tttcgtggcc tcgaggactt tctgcatgtc tgcgaaggcg
     4141 atcttcgcgg gatcggaagg tccggcaccg ccttcggaaa cgacaacgac tccgaccgcg
     4201 gcaatcataa ctaccataag aataacttgc agggtttttt tcacaagaac acctcctaga
     4261 attttttatt tgaatccaga agaatcgtaa ccgggccctc gttgacgagt tccacttcca
     4321 tatgagcctg aaagactccg gtctcaactc tcacaaattt agacgccagt ttgagaaaat
     4381 cgttgaagag gcccttcgcc ttttcgggat cggccgcgtc ggtgaacgac ggtctacggc
     4441 cctttctggc atcccccatg atggtgaact gggaaatggc tagcagggag ccgcccacat
     4501 cgagaaggga gagattcatc ttgtcgttcg aatcttcaaa gaccctgagg ttgactatct
     4561 tgtccaccag ccactccagg tcctctatcc cgtctgatgt tccgactccg atcagcgcta
     4621 acagtccctt atctatacta ccggtcgttc ggccatccac tctcacgctg gccctgctta
     4681 ccctctgaat gaccgccctc acatcactcc cccaatcctc ttgacgttct gcacgccctt
     4741 cattcctctc aagttttcca tgaccgatgt caactgggcc acgtccttca ccagaatgtt
     4801 cgccataagg gtatcgtatc cccacttccc cgtttccacc gagtacttct tgaccttggc
     4861 gctcttgctc tcgagcctct cgagaaacct gttcgccatc gctttgtcgc tgctgtcgaa
     4921 ctcgaccact atccacgcgc tgaacttctc gcctgtgatc atcgtccagt gggcctcgac
     4981 cagcttgtcc tgagagatcc ccttcacgtt ccggcagccg cggttgtgta tggagatacc
     5041 cctgctgctc atgacggcga cgatatcgtc tcccggaagc ggtgtgcagc acttggcgaa
     5101 atagacggct atccccgtct ctccaccgac cacgacctcg ttgcctttgg cctgcttctg
     5161 cttgacgagc tcggtctctt cgggaagtac ttcgacctct tctttcggaa ccagagcgct
     5221 gagaacctct cccatagtta ttgaaccatc tccgagtttc gagaaaaggt cgttttcgtt
     5281 gtgagctgcg aaacgcacca tgaggttctt cacttcttct ccctcgatga ggtcgtctat
     5341 ggatacggcc aggcgtttgc ttatctttct gaaaatctcc ttgccccttt cgataagctt
     5401 gaccgagtat tcattcttga agaaacgctt gatcttcgcc ttcgtcgagt tggccctcac
     5461 gtatttgagc cagtcgaggc tcggcccttc gctggccttg ttgaccacca cctcgacacg
     5521 gtccccgagt tgaagctcgt agttgaccgg caccagcctg tcgttgactt tggcgccggc
     5581 gtagtgatgg ccgatctcgg tgtgaatggc gtaggcgaaa tctatgggag tggagccttt
     5641 gggtagatgt accacctcgc ctttcggggt gaagacgtaa acctcttcca cctcgagctc
     5701 acgggaaatg gattccatgt ccttgaagct ttcgatgaaa tccttctgcc agtcgacgag
     5761 ctggctgaac caggctctgt ctttgacatc tatgccttct ttgtaaaccc agtgagaggc
     5821 gaccccgtac tcggcctctt tgtgcatcct ctcgcttctt atctgtatct cgagcggctc
     5881 gcccctgtgc gtaataaggg tggtgtggag agatttatag ccgttcgact tcggggcggc
     5941 gatgtagtcc ttgaaccttc cgggcatcgg aggccagagc gaatgcacag ctcccaggac
     6001 cttgtagcag ttgacttcgt cgcccgtaac gatcctcagg gctatgagat cgaagatctc
     6061 gtcgaaggat ctatctttct tgatcatctt ctgccatatg ctgtatagat gcttgacgcg
     6121 gccctctatg atggcggcta tcctgttctt tctgagttcc cggagtacga tgtttttgta
     6181 ttcgtccatt atctcctggc gttctttgag ctttttgttg accttcgcct tgagttctct
     6241 gaactcttcg ggatactcgt atttgaagga gaggtcctcg agttcggcct gtattttgtg
     6301 gatgcctatc ctgtgcgcga tgggggcgta gatctggatc gtttcgcggg ccttgtcctc
     6361 cctcttttcg gaactcttca ggaactcgag cgtcctcatg ttgtgcagcc tatcggcgag
     6421 cttaacgatg attatcctcg ggtccttgga gagggccaga agcatcttcc tgagcgtctc
     6481 gaccttcatt ctggacttca ctatcttcga gtccagcttt tcgttcaact tcaaattgct
     6541 tatcttggta acgccgtcga ctatgagcga gacggtctcg ccgaacttct cggttatctc
     6601 gcccaggggc acgttgcaat cctcgactat atcgtgcaaa aaaccggcga cgatcgtgtc
     6661 ggtgtccgcg gaaagctgcg ccagaatctt cgaaacctct acgggatggc ttatgaaggg
     6721 ttcccccgaa tctcgcatct gctcctggtg gtgaaagcgt gcaaactcgt aggcgtccag
     6781 aattctctct ttgtcgcgtc tcttgaggcg gtattgaagt atgctttcca gttcacccag
     6841 cagtatctgg gcatccaact cggttcttgc caccatctca ccttctatct agttattcta
     6901 gcatgaagcc ccggtccgtt gcagtggaaa ggagccgtgg tcggtggcga gttgtgggtt
     6961 gtcggtaaga gccgagatgc cggatcgggg tccggtacga cggaaagggg ttcgactggg
     7021 gcaggttcga agaaccggcc acgggtttcg ttagaaagta ccgtctgaaa gtcgaaaagc
     7081 gatcactcta gacggcgatc gcgaaagaaa tcccatatga gttcgctcgc tttgacactc
     7141 gatctggagc tgctctgaag ggcgtccagt cccttctctc ttcccggcca ggtgtggtct
     7201 ccatccacga tggtgtagaa gtggatctcg aagtcctctc ccggtctccc gtactttttc
     7261 agcaatacct ttccgctctc cagactcgtc tcttccagtt cgacggcctt catgccagcg
     7321 atccacaggt ctatcgattc cggtatggag ggaaagaaga cggtctcttc cgaagcggac
     7381 gagtagcctc catggtaagg tattatcgga tcgagcatcc cgtggaatat gatcatcggc
     7441 accggtctcg gcggcggctc gattctgtga acttcgtcct cgctcgcccc gccggccggc
     7501 gaagacgaga cgatggccgc ggccgcgaat agctcggggt aatcggacac cagtttgtag
     7561 gtcagcatgc cgccgttgga cagacccacc agataaaccc tgctctcatc gaccggatac
     7621 tcccgcttga gctccttcaa aagttcgact atgtatcgcg cgtcgtctat ttcattttcc
     7681 tttgcgtatg cgcagcaaaa gtcgccgttc cagctcagga aaacgctttt cagcgggccg
     7741 gtgccttcgg gataggccgc tatgaaaccc tctctgtcgg atatctcggt tattccgtaa
     7801 gtattcttgg tgtagttgct gtttccgagc agtccgtgaa acacgaagac caggggcagc
     7861 ttcgagtcgt cctcgccttt cggggggagg tggaccaccg tttttctatc gattccgtcg
     7921 tggttcaacc agaaggtctc gtcgacgccc ttttgtgaaa ctgcgaccaa gtagaacgcc
     7981 atcgcgatca acgtcgcggc tatcactatc gtcttcagca ttttatacct catctgaaag
     8041 tgttatgatt ctattttcct cagtatcttt tcgaagattc tggtcagttc ctgcgcttcc
     8101 ctggtatcga gaatctcgag gatcctgtgc gaaaaatccc tgtgtatttt gcggcaggcc
     8161 tggatgatct tttgtcccct gtttgtcagc tcaagccaga agactctcct gtcctcctcg
     8221 gactggattc ttttcaggta gccctcttca attaatcctc tcacggtgat cgtcacggtg
     8281 ggctttgtaa cgcttaggag gtcggctatc tggctgacgg tggggcgatc catcttcgct
     8341 atgacgttca gataatacat ctgccggacc gttagcctgg acgactctcc gctgtttaaa
     8401 gccctctctt cctgctccgc catgagcgtg gagagccttt cgacgatggt agttagcttc
     8461 tccttcacgc tggccacaat taaagcaact cccggattga ttactcatac ctgactaaca
     8521 atgatatcat tctccgaacg ggccagtcaa cgacttttgg agatggcaaa agagccggag
     8581 cgagatcccg ttcaggtgca caacgggatg acagtcttac tctcttcccg tcaggccgtc
     8641 ccggtgaagc ggcccacgac tatgcccaga atggtgaact gcggctccct gtttgcgatg
     8701 accacggtct cgaactcggg gttggaaggc ttgaattcga aggcgcccct gttcgggcgg
     8761 gaagatagaa acctaacgta cgattcctcc ctgtagcgaa agaagacgta cgagccgacc
     8821 ggcggaagca cgttctgctt tatgaaaaga gagtcccctt tgcggaaggt cggaaacatg
     8881 gagttgtcgg tgaactgaac gactaaatcg gccccgccgg tgaggtcgct cacgtaactt
     8941 atggcagagc tttcatcgag ccgatcggtt ctctccagcg tgaagacggg gatgaccctc
     9001 ctgatcactc ccttatggac cggctgggga gacgaaggct ctatcgagcc gtagatagag
     9061 acgccgggag tatacagctt ttccggcacg ggcttctcgg cgctttttgg atctgcgtgt
     9121 ttgggaatta gaacgtcgag catcgtggag ctcagctttt ccctggtctt cgtttcgtac
     9181 tcaaaggtat atctgtcggg caacccgagg aggtaatcgg ccgaaacttc gaagtacttc
     9241 gcgattctgt atatcatgtc ggtcggcggc agccttttgc ccgactcgta gagcgagatc
     9301 aggggtacgc tcttcccgag agcctccgcg agctgctcct gggtcattcc cccggacact
     9361 ctgagattct ctatcctctt tccaagtaaa tctgctttga atgtattcat gtgagaatga
     9421 tatcaccaac ttgtctaaaa gtcaatcaaa ttaccccctt tacaaaatcg ttttttactg
     9481 ataatatata gtgtgttgac ttaaagcaac aagtccacgc gttgttatca tgagtaaagt
     9541 aaccgcctat aattgtctaa tatagagacc ttcagcaact aagacagtga gaacacggct
     9601 ctcaatcaga gcagagtagg aggaaatttg gcagaagaaa agaaaaatgg atctccgtcg
     9661 agagatccca gtaaagacag atattatttt aggcagaacg attataccac gcgagaagga
     9721 aaaagaccag cgacaaaaca ctccgacaag atcccgtcca gggaagatta tcgcctgtac
     9781 cacgcacagc tcagggagat ctacgatagt tgtaagtctt gtgatgaggc cttcaccgaa
     9841 gggagggagc taattgacag actcgaaatc tagaccggca tcccataaat cgattctcag
     9901 agtgaagaag accaacaact acgtggtgct ggacaaaggg ttcctgcagg acccttcgct
     9961 ttcctgcaag gccaaagggt tgctgagcta tctgctctcc aagcccgacg actgggagat
    10021 aaggctctcc gagctatgca gacacttcag cgacgggaag gacagcataa ggaagggcat
    10081 cgaggaactc gaaaaggccg gctacatcaa aaaggaaagg cttaggggag gaaacgggaa
    10141 gttcgccggc gtcagataca tggtctatga aacgccctgc ctgagccaga aggaaccgca
    10201 aacggaaaaa ccggatacgg ttttcccggc tccggaaaac ccgacgctac taaataatga
    10261 tctcactaag gatacttcgt atcgtgtggc cacgaacttt gaagtatcgt tcatgagcaa
    10321 gagagaatgt atcaagaaaa cggcgcgcct gatcgaggat ctcggtgtag agaacagcta
    10381 caaagtagcc gcagcgatgt tcagaaacgc cgacagagag atagactggc acttcccgat
    10441 ctggatcctc gaaaacctca acgaagcaat cgagagggcc gacgacaagg tgaagtacct
    10501 gctctcgctc ctgcaggacg acaacctctg gcactgctat gcgagccagc tggaagatga
    10561 agctttcgat gaaaggagag tgcttgccaa tgttgcagct gggtattgaa ggcgaaagaa
    10621 ctctactaaa ggcccttctg gagtacgaag agctgagatg cagaatagac gagatagatc
    10681 cgagttacct cagcgaagaa tcgaggaaga tcttcgaagt gatctccgac aacagagaga
    10741 tggacagcac caggctgtgc gtccagatat acaacaaaat cggaaaccag aagttctacg
    10801 acggggattt catcactgag gtcggcgcca actatctgat cgacgattac aaagagctcg
    10861 tcggaaaaaa cgggctccag cgcctgtgcc tggagatctc gaaaaagctg aaaaaaggcg
    10921 acatgaaggt cgatgaggcg atggagaagt tctacgcctt ccagaaggaa agctcctcgg
    10981 ccttcaacta cataagcatc aaagaggcga acgaactcca gaagaaggcg catacggaca
    11041 tctggaacaa caggggcaat cgacttcact ggcccttcaa attcttcgaa gacattctca
    11101 acggcctgag aggaaaggag ctggtcgtcg tggccggcga gacgggaact ggaaagacgg
    11161 ccttcatgtt gaacgcggcc gccctttggg cgagcttcgg aaagaccgtc ggatacatct
    11221 cgctcgagat agatgtcgta gatctcaagg acaggatgca ccagaaagac ttcgcggtca
    11281 acctcagcgc agaatcggag aaactggacg ggcaggagcg ggagagactg ctcagggaga
    11341 tcgacaagac gggcgagcag aggctcttct tctgcgacag cggtgtcagg tctgtccaca
    11401 gcatcatagc cgggatcaag ctgatgcacc atatacacag attcgacgtg gtattcatag
    11461 actatctgca gctcatgacc tgcccgggga aacagaccaa agatatggaa ataggcgaga
    11521 tcatgggttc gctcaaggcg ctcgcgatgg agataagggt gcctataata gtcggctcgc
    11581 agctcaacag agaggtcggc aaaaacgaca gggggatccc caggctatcc aacctcagag
    11641 aatctggaac gatagaacac agcgccgacg tggtcatact gctgtacagg ccgatcctct
    11701 acgacagaaa cgcctcgccc gatgaatacc aggccatagt ggccaagcag agaagcggca
    11761 gaaccggaac gctctacatg cacttcgaca tgaaaagaca gatcatgcag ggagtcgtcc
    11821 ccggcgagcg agcctacgcc agataattcc agatatactc accacccgca acccgcaacg
    11881 gctttcgggt aactcgcatg atttttctgg gctttatctg ttagaatttt cattattcca
    11941 tcagaccggg agttatttta tgaggcttaa cgaaaatctc tttgaaagaa ggctcgggct
    12001 tttcttcatg gttctgtggt tcgtggttat gttcttctac atcacctggg acggctggct
    12061 gtacgagtgg cgcgactgcg gttatgaagg accgaaccag cacttcgatc tggatatgtc
    12121 cgacatggat tatgaataca cggccgatgg cgtgaagatc taccactcga ccaacaacct
    12181 gcacaaccta atttatctct gttatttcgt gttttttttc ggctggctgg gtttgctttt
    12241 cgacatcgag tgggccaagc agtggacgat ggcgaccacc gggatatccg tcgtcgcggc
    12301 cctttcgact ttgaggctct ccgatcacgt gtatatactc caaatcgtct acgatgcggt
    12361 tcacctttcg gggatagtca tggggtgtta ccttttctgg aagtacgatc tgaagatccg
    12421 aagagccgtt cacgcggtgc tgttcacgtg ggccgtttac ctgtcgagcc acctgatctt
    12481 caccccgtgg ccttactggg agaaccaggg aaaggcttac ttcagcatta atcagatcaa
    12541 cgatctgccc ttcttcattt tcgggctgga gtacttgcta gtcgtggccc ttctgctcgg
    12601 tatagacgta gcggcaagtt tcataaactc caggctcgac aatagatttc tcaagatggc
    12661 cgtgccgctc ggcctctacc tgtcgctgtg catcatcctg atagctctcg gtctgataat
    12721 cgtgcctgag attcacataa acacctgccc ttaggctcat ataggaaact tcaactgtcc
    12781 caggtcgccg ctctttctga ggatctgaac ggcgaccagc tttcccttag aatcgaaata
    12841 tttgatcgct ctttcacgtc ttttcgactc gtctggactt aatagaatat attcgtaacc
    12901 ctcccagacc ttccagcgtt tcatttaaac ccctccaaaa tgagcccaca ggaaaaatag
    12961 tatcatttat a
//
LOCUS       DAOOLY010000042        12953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5308_length_12953_cov_4.693829, whole genome shotgun sequence.
ACCESSION   DAOOLY010000042 DAOOLY010000000
VERSION     DAOOLY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5308_length_12953_cov_4.693829"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLD15_03575"
     CDS             complement(<1..595)
                     /locus_tag="PLD15_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPB63012.1"
                     /translation="MGDLRRTELVNLVTFIFGSYIDYRLQEVQPILERRFGPVDYISK
                     TLDFDRFTSFYNDEMGYKLSGKLLSFKRLIHPQQLSLIKHITNTIEIDLSVDGKRKVI
                     KTSAKYGKHQVLHEESERIKIREIDQDKEKEIRVYEVKTIPGTKRIAVYLLPQDAHIY
                     DIADMSEVSQGDVLSRVPKGTTKMKDITGGLPRVTELF"
     gene            complement(596..931)
                     /locus_tag="PLD15_03580"
     CDS             complement(596..931)
                     /locus_tag="PLD15_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63013.1"
                     /translation="MAKRGNSNDQMIVILLLVISLFLFVESLFLYRFVKDTMAVEYEI
                     EFQSLSAELRILIGDEGYRLVSGDSLTIKVHRNEKVVVRQETPGRYTIKSGEKMVSVT
                     IPQVEVSVR"
     gene            1090..1740
                     /locus_tag="PLD15_03585"
     CDS             1090..1740
                     /locus_tag="PLD15_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPB63014.1"
                     /translation="MDNQKIPKPTIKRLAVYHRCLENMLFSGKASVSSKDIADQLGIK
                     ASQVRKDLSYFGEFGRRGVGYNTQQLLDSVSDILGMRKVWNVCIVGMGNLGMALATYP
                     GLGRSGFFIRALLDNNPNKIGIPIPNDLSIEPISVLKSVVKERAINMGVITVPAEVAQ
                     ETADALVDAGVQGIVNFAPVRLNLPDSVNVEEIDISVSFRSLAFSISLHSGRRRRE"
     gene            1740..2345
                     /gene="udk"
                     /locus_tag="PLD15_03590"
     CDS             1740..2345
                     /gene="udk"
                     /locus_tag="PLD15_03590"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPB63015.1"
                     /translation="MVLVSIIGGSGAGKTSVTNVIYNHFNDRAAVLSMDDYYKNLEPG
                     TDPREFNFDSPKAFDFELFESHLRTIKENGTVKVPVYSMVTYRREDGIWRTFEPRPLV
                     LVEGLLVMFRKEMRELFDFSIYIDAPADERLIRRIERDTRERGRSLESIITQYRKFVS
                     PSFESFIEPQKYFCDIVLPDGAANTTGLNVIINAIENMLKK"
     gene            2365..4686
                     /locus_tag="PLD15_03595"
     CDS             2365..4686
                     /locus_tag="PLD15_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63016.1"
                     /translation="MPKRFLTVVLLLAVSVLSFSFSQEEVLDRFKSYMNDYQREAPEL
                     QKIKKLEEDLNYLSVYRLYKLQTVGSIEKKESATTIADLLSRHLESLPAEDFSSNDDR
                     IAYSAFLAWVLSDFSGKAFQVGTLNEMPAYLSTFNSFTSQVRSMAEAVYKEWMAYALG
                     LVKDEPSVFPGELKKTDTFAQYSLKADADEKSEREILSLSSNQIYNLLNSSIDTIGKR
                     EYDISSLVEEEVKRFAVQATLDVAPLMDSNLMNAARDLFQLWLYRSLGLVEEVPHYPA
                     EISVKTLSIKGFNLSLPLDNPDYERVVEILNNNLDSRLKSIEKLQMASQVLSIRQFTP
                     VGLIERDIGDEVKKIIPVQAGILGQLRNSLSREIVSVSEKGVNLWWLRFVGYIILALI
                     AFFLLPALRKYWLGIVITFEIFYMLFLTDVTRNLFDLSLYSIIVLPVFAFILIMAVFS
                     IFKKGGKKSLLVIKLLLLATIAIFPFLKLYNDVPEISMDSFEGFYDSIYYSTLKRDLF
                     LAPESLISLEIRDLSSVVSSELNSFKRVLRVIVPNEMNAFSNNAGLSYTVDGNGRLRV
                     TAPAFSEYMSIENQQAYADELRGLSKDLNSFIRDSERNARTYESLLKSFVSSSERIIR
                     YSGETLRADFTEFVETSLKSKPELNVVMDDYLAEVSDDLEREALPAVVRVFRVPKFVA
                     LALGLFLLSAVVFVVKKPLISLINIVVVSVYFVISLAGIKELTLFVQGGSPVLNITVD
                     PSVNALFLIVFAGIIVLSVLWVLLSQKKGSVSE"
     gene            4683..5705
                     /locus_tag="PLD15_03600"
     CDS             4683..5705
                     /locus_tag="PLD15_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB63017.1"
                     /translation="MKLRVLLVISLILMVALPVMAMKVIMVTDVGGLGDKSFNDGTWE
                     GIVKASDFLGLEKEVVQSKEQSDYIPNLSNAAKEADIIFGVGFMMADALYKVAPQFPD
                     TYFIGIDIDPIENMPDNVATYLFKEQEGAFLVGYVVAAMTKTNMVGFVGGIPIPPVER
                     FRYGYEAGIRVYEELHGKTISMLQGYTMDFNDPKKGKDLALAQFAEGADIVFHASGAC
                     GNGVIEAAAEKGEGFFAVGVDVDQDYMAPGRVLTSSVKRVDMASYQAVMSIALGTFEP
                     GTKTLGIKDEGVGISPMKYTKDVVGPIILSEIEFLREQIKAGTIVAPDTQEKLDAFVV
                     PAIALP"
     gene            5797..7380
                     /locus_tag="PLD15_03605"
     CDS             5797..7380
                     /locus_tag="PLD15_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63018.1"
                     /translation="MDATNVKNLDLSGMAVAMKGITKRFPSVVANDNVDFFVKKGEIH
                     ALVGENGAGKSTLVNQLYGLYTPDEGEIYINGKKFVFKGPRDAIDAEIGMVHQHFMLV
                     DNQTVSENVVLGSEPRRGPFNLFIDSGKSRRSVVELSKKYGLLVDVDAMIEDIPVGMQ
                     QRVEILKILYRGASILIFDEPTAVLTPQETEELFKILRVLKENGKTIIFITHKLNEVM
                     AITDRVTVMRLGKVTGQVDTRDTNPRQLANMMVGREVLLRVEKEAHEPGKTLLEVKNL
                     HVKDNRKLDTVKSVSFSVREGEILGIAGVAGNGQTELIEAITGLRKAESGSITLMGKD
                     LTNKSALDIRESGLTHIPEDRLKRGLISQYPVYYNLILGKHNDKPFASEGFIDMKEVK
                     NFARYIVETFDVRPKDIDILGGNLSGGNQQKVVVGRELSTVPIEPKVVAISQPTRGLD
                     IGAIEFIHRTILNMRTKGIGILLVSMELDEIFSLSDRILVFYEGEIMGEVLPNEVNRE
                     EIGLMMAGHSKAEVVKERG"
     gene            7387..8442
                     /locus_tag="PLD15_03610"
     CDS             7387..8442
                     /locus_tag="PLD15_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63019.1"
                     /translation="MNRSKIYALLVPVVSVIIALLIASIIIIIIGKNPLTAYGIMLEG
                     AFGGAEQLANTIIKMTPLILTGLAVGFGFRAGVFNIGAEGQMTIGALMGAVVAMNIGG
                     VPSVIAIPLSIIVGMLAGAFWASIAGFLKAFTGAHEVISTIMLNWIAANFASFMVTGP
                     LAVGSGTPKSPEIAEAAKLPVVLKVQATELSIGIFIAVVVAVVMYVFIEKTTTGYELK
                     GVGFNPHAAEYGGISIRKSIIMTMAISGALAGMAGIVDLLGVPPHRFVGELTGGRGFD
                     GITIALIGRNNPIGIIFAALLIAALRTGSNAMQIRAQIPNDIVSIIQGIVIFLVAAER
                     IVTTLIYWKGRKGVTTA"
     gene            8439..9398
                     /locus_tag="PLD15_03615"
     CDS             8439..9398
                     /locus_tag="PLD15_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63020.1"
                     /translation="MSWIEAIFAILVNPLFYKLTLLSATPLIFSALGGTYSEITGVTN
                     IALEGIMLMGAFNSIVFTFITGNAWIGVLMAVIIGVGFTWFHAWASIRWSANQIVSAT
                     ALVIVAQGTTSFLMIPIFGNEGQTDFIGRVPYIEIGWLKGIPFIGDIFGSMSPFTYLA
                     IAAVIASWILMYRTPLGLRMRAVGENPEAADTLGINVFKTRYFGVLMSGALSSLGGAF
                     LSVGELGRFVENMIHGRGFIALAAMILGNWNPAGAMWAALLFGAAEAMNLQLQSSSIV
                     SVSANVKPLFNMLPFIVTLIVVGGFIGKTRSPAADGEPYEKES"
     gene            complement(9424..10878)
                     /gene="gltX"
                     /locus_tag="PLD15_03620"
     CDS             complement(9424..10878)
                     /gene="gltX"
                     /locus_tag="PLD15_03620"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB63021.1"
                     /translation="MKMNRVRFAPSPTGYLHVGGARTALFNYLFAKHYDGKFVLRIED
                     TDISRSTKEYEEQLMESLLWMGITWDEGPDVGGETGPYRQTERTDIYVKMVDELINSG
                     KAYRVYAYPEEIETLHDDLLSQGKPPHYDQQMLEKFNTPERKKEFEGGGLNPVVFFKM
                     PRKEYRLLDLIKGEVIFKEGAIGDFVILRSNNQPIYNFAVVVDDILMEITHVIRGDDH
                     LSNTLRQLALYEAFEAPIPSFAHVSMILGPDGKKLSKRHGDTSVEQFRDRGFLPEAFF
                     NFLTLLGWSHPEGKEILSYGEIIESFTIDRVNTSAAIFDEAKARWMNGVYIREADLDR
                     ITDLTIPFLVQAGLISLEEAGANLKWLKRAIDSVRKGVELLSEIPEKMKLYFEEIQVR
                     PLSPENEEQRKVFRSLEAFKDRIEYLNSWSNEEIVSTIKEILKEIKPDRKSFYMSLRH
                     VLTASEEGPELVDVVFLLGRNKTLHRLQQALRLP"
     gene            complement(10878..11405)
                     /locus_tag="PLD15_03625"
     CDS             complement(10878..11405)
                     /locus_tag="PLD15_03625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB63022.1"
                     /translation="MEISLHKEEIFSGRILKLERHLVKLIDDSEASREVVRHPGAVAV
                     VAFKGEELLLVRQYRFPVQKYMLEIPAGKLDEGEDPVNCARRELLEETGYGPVRLEKL
                     TVIETSPGFSDEAIHIFTCEVERVGESDPDEGEFVEPVFMRIEEVVKRILEGSISDSK
                     TISGVLLALRRKESL"
     gene            complement(11407..11799)
                     /gene="cdd"
                     /locus_tag="PLD15_03630"
     CDS             complement(11407..11799)
                     /gene="cdd"
                     /locus_tag="PLD15_03630"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPB63023.1"
                     /translation="MKDSSKENILIQKAIEAMENSYSPYSNFPVGAALLTVEGEIFTG
                     TNVENASFGLSICAERSAIASAVSRGRRSFRAIAIVSKRDEATSPCGMCRQFMAEFGD
                     FEVILVGKDSVVRTTVFELLPMHFELEE"
     gene            complement(11753..>12953)
                     /locus_tag="PLD15_03635"
     CDS             complement(11753..>12953)
                     /locus_tag="PLD15_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB63024.1"
                     /translation="SKSVKNFIDNPNSILTAILVMNNIVNILASSLATLLAIQLLPQN
                     SAGVAAAIVTGVMTFLILVFGEITPKIFARENPERFFKRVIVFITILTSVLRPLLWLL
                     LRISNLFIVIVGGKKVPYAPFITEDEIRSAVDAGHEEGVLQSEERMIMKRTLELKDIS
                     VKEIMTPRVEIVSLEEDAPLIELMELVESEGYSRYPIYRENVDRIVGVCYAKDLLNFI
                     LDRKDNDLLQVVHVKEIMRAPYFVPETKKVDDLLREFKEKKNHMAIVIDEYGGTAGLI
                     TMEDAIEELTGEILDEYDEESEEMMIEKVSDSEYIVDGMTPINDIERELEQVFPDTEF
                     ETIGGYLLEVLERFPEVGEKIIVNGFTFEILAAGKNKVEKIRLSVDRRSVDERQFKGE
                     HTDTESY"
BASE COUNT         3211 a         3190 c         3244 g         3308 t
ORIGIN      
        1 caaacaactc ggtaaccctt ggcagaccac cggtgatgtc cttcatcttg gtcgttccct
       61 ttggaaccct cgacaggacg tcaccttggc tgacttcaga catatcagcg atgtcgtaga
      121 tatgtgcatc ttgcggcagc aggtatacag caattcgctt tgtaccagga atcgtcttga
      181 cttcgtagac tcggatctct ttttccttgt cctgatcgat ttcccttatc ttgattcgtt
      241 ccgattcctc atgcaggacc tgatgctttc catacttggc actggtcttg atgacctttc
      301 tcttgccatc aaccgacagg tctatctcga tggtgttggt gatgtgtttt atcaatgaaa
      361 gctgctgtgg atgtatcaat ctcttgaaag acagtaactt ccccgagagt ttgtacccca
      421 tctcgtcgtt gtagaaagaa gtgaatcgat cgaaatctag agttttggag atgtaatcta
      481 ccggaccgaa ccgcctttca agaataggtt gaacttcttg gagtctgtaa tctatataag
      541 atccgaaaat aaacgtaacc agattcacca gctcagttct tctgagatct cccatttatc
      601 tgacactcac ttccacttgt ggaattgtca cgctgaccat cttttcacca cttttaattg
      661 tatatcttcc cggggtctct tgccttacaa ccactttctc attgcgatga acctttatgg
      721 tgagactgtc tccagacacc aacctgtagc cttcatctcc gatcagtatt ctcaactcgg
      781 ccgacaacga ttggaactct atctcgtatt ctacggccat cgtatctttt acaaagcgat
      841 agagaaaaag agattcgaca aaaagaaaaa gcgaaatgac caacaacaaa attactatca
      901 tctgatcatt ggaattgccc ctctttgcca taaaacagaa ctcccaccat tctccaggta
      961 aaggactaca gcaattatag catgggaaag cactacaatc tgagagccca acactggggc
     1021 cgtagagaat ccacacgtga tataattttc acaaatggaa tctctgtgat tttcaggggg
     1081 ttgatcggat tggataacca gaaaattccg aaaccaacca taaagagact ggccgtgtat
     1141 cacagatgtc ttgaaaatat gctcttttct ggtaaggcga gtgtgtcgtc caaagatatc
     1201 gccgatcaac tcggcataaa ggccagccag gttcgtaaag acctatcgta ttttggcgag
     1261 ttcggaagac gcggagtcgg ctacaacacc cagcagttgc tagacagtgt cagcgatatt
     1321 ctgggaatga gaaaggtatg gaacgtgtgc atagtaggta tgggaaattt aggaatggcc
     1381 ctggccacat atccgggact gggcaggtcg ggctttttca ttagggctct cttggacaac
     1441 aaccccaaca agattgggat tcccattccg aacgatctct cgatcgagcc catttcggtc
     1501 ctgaagagtg tcgtgaaaga gagagcgata aatatgggag tgatcacggt accggccgag
     1561 gtggctcagg aaacggccga tgcgctggtt gatgcgggag tacagggaat agtgaacttc
     1621 gctccggtaa ggttgaatct gcccgatagt gtgaacgtag aagaaataga tatttccgtt
     1681 tccttccgat ctctggcctt cagcataagt cttcactctg gcaggaggag gagggagtaa
     1741 tggttctggt ctctataatc ggcggaagcg gtgccgggaa gacctcggtc acgaacgtta
     1801 tatacaacca tttcaacgac cgggccgccg tactttccat ggatgattac tacaaaaacc
     1861 tggaaccggg gaccgatcca agggagttca atttcgattc gcccaaagcc tttgacttcg
     1921 agctcttcga gtcgcacctc agaactataa aggagaacgg tactgtgaaa gtgcctgtct
     1981 atagtatggt gacatacaga cgagaggacg ggatatggag aacattcgaa cccagaccgc
     2041 tcgtgctggt cgagggattg ctggtgatgt tcaggaaaga gatgagagaa ctgttcgatt
     2101 tctcgattta catcgatgca ccggccgatg agaggctgat caggagaata gagagagata
     2161 ccagggagcg tggtcgttca ctcgagagta taatcactca gtacaggaag ttcgtctctc
     2221 cgtcgttcga gagtttcata gaaccccaga agtatttttg tgatatagtt ttacctgacg
     2281 gcgctgccaa cacaacaggg ctcaacgtca ttatcaatgc catagagaac atgctaaaaa
     2341 agtgatcggg gctggaggtg tgaagtgcca aagagatttc tcacggtcgt tcttttacta
     2401 gccgtttctg tactctcctt ttctttcagc caggaagagg tactggatag attcaagagc
     2461 tatatgaacg attatcagag agaagctccg gaactccaaa agataaaaaa gctggaagag
     2521 gatctgaact atctctccgt ttacaggttg tacaaacttc aaacggtcgg tagtatcgag
     2581 aagaaggaaa gtgcgaccac catagccgat cttctttcaa ggcatcttga atcgcttccg
     2641 gccgaggact tttcgagcaa cgacgacagg atagcctaca gtgcctttct cgcatgggtg
     2701 ctctcggatt tctccggcaa ggctttccag gttggaactc tgaacgagat gcccgcctac
     2761 ctttcgacat tcaactcctt caccagtcag gtcaggagta tggccgaagc tgtctacaaa
     2821 gaatggatgg cttacgcgct gggcctggtc aaagatgaac catcggtctt tccgggcgag
     2881 ctgaaaaaga ccgatacttt tgctcagtac tctctaaaag cagacgccga cgagaaatcc
     2941 gaaagagaga tactctctct gtccagcaat cagatttaca atcttctcaa ttcgagtatc
     3001 gatactattg gcaagcgcga gtacgacatt tcatcgctgg tagaagagga agtgaagaga
     3061 ttcgccgttc aggcgacgct ggatgtggct cccctcatgg attccaatct gatgaacgct
     3121 gcgagagacc tattccagct ctggttgtac agatcgctcg ggctggtcga ggaagttcct
     3181 cactatccgg ccgaaataag tgttaaaacg ctttctatca aagggttcaa tttgagttta
     3241 cctctggata atccagatta cgaaagagtg gtcgagatcc tcaacaacaa tctggattcg
     3301 cgtctgaagt ccatcgagaa gctacagatg gcttcgcagg tactttccat cagacagttc
     3361 accccggtag gtctaataga gagggacatt ggcgacgaag tgaaaaagat aatccccgtt
     3421 caggccggca tcctggggca gttgcggaat tcgttatcca gagagatcgt ctccgtgagc
     3481 gaaaagggcg taaatctctg gtggttaaga tttgtaggtt atataattct agctttgata
     3541 gcctttttcc ttcttccggc gctcagaaaa tactggctag ggatcgtcat taccttcgag
     3601 atattctata tgcttttcct gaccgacgtg acgaggaacc tcttcgatct ctcgctgtac
     3661 tcgataatag tgctaccggt cttcgccttc attctgataa tggccgtctt ctcgattttc
     3721 aagaagggcg gaaaaaagtc gctgctagtg ataaagcttc tactactggc gacgatcgcg
     3781 atctttccgt ttttgaagct ttacaacgat gtaccggaga tctcgatgga cagtttcgaa
     3841 ggtttctacg attcgatcta ttattcaact ctcaagaggg atctcttcct cgcacccgag
     3901 tctctgataa gcctcgaaat aagggatcta agttcggtcg tctccagcga attgaacagc
     3961 ttcaagagag ttttgagagt catcgtgccc aacgagatga acgcattttc caacaacgcc
     4021 ggtctatcat acaccgttga cggcaacgga agactaagag taaccgcacc ggctttttca
     4081 gagtacatgt ctatcgaaaa ccagcaagcc tacgccgatg aactccgtgg attgtcgaaa
     4141 gacctgaaca gtttcataag agacagcgaa agaaacgcca gaacttacga gtctttgctt
     4201 aagagtttcg tatcttcttc cgagaggata attagatatt cgggagaaac actgagggca
     4261 gacttcactg aattcgtcga aacctcgctc aagtccaaac ccgagctgaa cgtagttatg
     4321 gacgactatc tcgctgaggt gtccgacgat ctggaaagag aggctcttcc ggccgttgtg
     4381 agagtcttca gggttccaaa attcgttgca ctggcactgg gtctcttcct gctctcggct
     4441 gtcgtttttg tagttaagaa accgttgatc tcactgatca atatcgtcgt tgtgagtgtt
     4501 tatttcgtga tatctcttgc cggaatcaaa gagctaactc tattcgttca aggtggctcc
     4561 ccagttctca acataactgt tgatccgtcc gtaaacgctt tgtttttgat tgtttttgca
     4621 ggtataattg ttctatcagt tctatgggtt ttactatctc aaaagaaagg gagtgttagt
     4681 gaatgaaact cagagtgctt cttgtcattt ctcttattct catggttgcc ctcccggtaa
     4741 tggcgatgaa ggtaatcatg gttaccgacg tcggcgggct tggcgacaaa tcattcaacg
     4801 atggaacctg ggaaggaatc gttaaggcat ctgattttct cggactcgag aaagaggtag
     4861 tccagtccaa agagcagtcg gactacatac ccaacctctc caacgcggct aaagaagccg
     4921 atataatctt cggagtcgga ttcatgatgg ccgatgctct ttacaaagta gctccccagt
     4981 tcccggacac gtacttcatc ggaatcgaca tcgaccctat cgagaacatg ccagacaacg
     5041 tggcaacgta tctcttcaaa gagcaggaag gagccttcct tgtaggttat gtcgttgcgg
     5101 caatgacgaa gaccaatatg gtgggtttcg tcggcggaat cccgataccg ccggtcgaga
     5161 ggttccgcta cggttatgaa gccggaataa gggtctacga agagcttcac ggaaagacaa
     5221 tctccatgct tcagggatac accatggact ttaacgatcc caaaaagggt aaagacctcg
     5281 ctctggcaca gttcgcggaa ggcgcagata tagttttcca cgcttccggc gcctgtggaa
     5341 atggagtaat cgaagctgct gccgagaagg gcgaaggctt ctttgcagtc ggtgttgacg
     5401 ttgaccagga ttatatggca cctggaagag ttctaaccag ctcggtaaag agagtcgata
     5461 tggcttccta tcaggcggtc atgagtatcg ctctgggaac tttcgagcct ggcaccaaga
     5521 ccctgggtat aaaagatgag ggtgtcggaa taagcccgat gaagtacacc aaggacgtcg
     5581 tcggtccgat cattctcagt gaaatcgaat tcttgaggga acagatcaag gccggaacca
     5641 ttgttgcccc cgatacgcag gaaaagctgg acgctttcgt tgttcccgct atagcgctgc
     5701 cataatggtt tcttttcaca caatcgtgtg atatcaaaga tcgggagggt taacgctctc
     5761 ccgatctttc gtatcatgaa cgatgggggt gagcgtgtgg acgccacaaa cgttaaaaac
     5821 ctcgatcttt ccggtatggc cgttgccatg aagggaatca ccaagagatt tccctccgtg
     5881 gttgccaacg ataacgtaga tttctttgta aagaaaggcg aaatacacgc gctcgttggg
     5941 gagaacggtg caggaaaatc cactctcgtg aaccagcttt acggtcttta tacccccgac
     6001 gaaggcgaga tctatataaa cggaaagaaa ttcgttttca aagggccgag agacgccata
     6061 gacgcggaaa taggaatggt tcaccagcac ttcatgctcg ttgacaacca gaccgtctcg
     6121 gaaaacgtcg ttctcggttc cgagccgaga cgtggtccct tcaacctctt cattgacagc
     6181 ggtaagtcga ggagatccgt cgtggaactc tccaagaagt acggtctgtt ggtcgatgtc
     6241 gatgcgatga tagaagatat accggtcggt atgcagcaga gagtcgaaat tttgaagatc
     6301 ctttacagag gtgccagcat actgatcttc gacgaaccga ctgcggtgtt gactccccag
     6361 gagacagagg aactcttcaa gatcctgaga gtcctcaaag aaaatggaaa gaccatcatc
     6421 ttcatcactc acaagctgaa cgaagtgatg gctataactg acagagtaac ggttatgcga
     6481 ctgggaaagg tcaccggcca ggtcgatacc agagatacca accccaggca actggccaac
     6541 atgatggtgg ggagagaagt gctgctcaga gtcgagaaag aggctcacga gcccgggaag
     6601 acgctcctgg aggtcaaaaa cctccatgtg aaagacaaca gaaagctcga taccgtcaaa
     6661 agcgtatcct tcagcgttag ggagggcgag atactgggaa tcgccggagt ggccggaaat
     6721 ggccagacgg aactcataga agccatcacg ggattgagaa aggccgagag tggcagtata
     6781 actctcatgg gaaaggatct cacgaacaag tcggccctcg acatcagaga atctggattg
     6841 acacatattc cagaagacag gcttaaaagg ggcctgatct cacagtaccc ggtctattac
     6901 aatctgatcc tgggaaagca caacgacaaa cccttcgcgt ccgaaggttt catcgatatg
     6961 aaagaggtca aaaatttcgc caggtacatc gtcgaaacct ttgatgtgag gcccaaagac
     7021 atagatatcc tgggtggtaa cctttcaggt ggaaaccagc agaaagtcgt agtggggagg
     7081 gaattgagca ccgttcctat agagccgaag gtcgtggcga tatcgcagcc gaccagggga
     7141 ctcgatatcg gagctattga gttcatacac agaacgatac tgaacatgag gacgaaaggt
     7201 ataggcatcc ttctggtctc catggagctg gacgaaatat tctccctctc agacaggata
     7261 ctggttttct atgaaggtga aataatgggt gaagtgcttc ccaatgaagt gaaccgtgaa
     7321 gagataggac tgatgatggc cggtcacagc aaggcagaag tagtgaaaga gcgggggtga
     7381 ttgagaatga accgaagcaa aatctacgct ctgctggtac ctgtagtttc tgtaattatc
     7441 gcgctactga tagcttcgat cattataata atcattggca agaatcctct gactgcctac
     7501 ggaataatgc tggaaggagc tttcggcggt gccgaacagc tcgccaacac tataatcaag
     7561 atgactccgt tgatactcac cggtctggcc gtcggtttcg gtttccgcgc cggtgtcttc
     7621 aacatagggg ccgaagggca gatgactatc ggcgcactga tgggagcggt agttgcgatg
     7681 aacataggcg gtgttccttc ggtgatagct ataccgctat ccataatagt cgggatgctg
     7741 gcaggcgcct tctgggcctc gatagccggt tttctgaagg ccttcaccgg cgctcacgaa
     7801 gtcatttcga ccataatgct caactggata gcagccaact ttgcctcttt catggtcacc
     7861 ggaccgctgg ccgtgggctc gggtacgccc aagagccctg agatagccga agccgcgaag
     7921 ctgccggtcg tcctgaaggt tcaggcgacg gaactgagta taggcatctt catagccgtt
     7981 gttgtggcgg tggtcatgta cgtcttcatc gagaagacaa cgaccggtta cgaactgaaa
     8041 ggtgtgggtt tcaaccccca cgcggccgag tacggcggca tcagcataag aaagagtatc
     8101 ataatgacga tggccatcag cggagcgctt gccggaatgg ccggtatagt ggaccttctc
     8161 ggagttccac cccacagatt cgtgggtgag ctgaccggcg gacgcggctt cgacggaata
     8221 actatagcgc tcatcgggcg aaacaatccg ataggcatca ttttcgccgc tcttctgata
     8281 gcggctctga gaacagggtc caacgctatg cagataaggg cacagatacc gaacgacata
     8341 gtttccataa tccagggaat agtgatcttc ctggtggcgg cggaaagaat cgtcaccacc
     8401 ctgatctact ggaaaggcag gaaaggggtg accacggcat gagctggatt gaggctattt
     8461 ttgcgattct cgttaatccg cttttctata agttgactct gctttctgcg acgcctctga
     8521 ttttctccgc cctgggcggt acttacagcg agatcacagg ggttactaac atcgctctcg
     8581 aagggatcat gttgatgggc gccttcaact ctatagtctt cactttcatc acggggaacg
     8641 cctggatcgg cgttttgatg gcggtcataa taggggttgg gttcacatgg ttccacgcgt
     8701 gggcgagcat acgctggtcg gccaaccaga tagtcagcgc aacggccctg gttatcgtcg
     8761 ctcagggtac cacctctttt ctcatgattc ccattttcgg caacgagggt cagacggatt
     8821 tcataggaag ggtaccctac atagagatag gctggctgaa gggaataccc ttcatcggcg
     8881 acatcttcgg ctctatgagc cccttcactt atctggcgat cgccgcggtt atagctagct
     8941 ggatcctgat gtacaggacg cctctaggtt tgaggatgag ggcggtcggt gaaaaccccg
     9001 aagcggccga tactctaggt ataaacgttt tcaagaccag atatttcggt gtcctcatga
     9061 gcggagccct gagcagtcta ggcggagcct tcctctcagt gggagagctg ggaaggttcg
     9121 tcgagaacat gatccacggg aggggcttca tagctctggc cgcgatgata ctagggaatt
     9181 ggaaccccgc gggcgcaatg tgggcggccc ttctctttgg tgccgccgaa gccatgaacc
     9241 tccagctgca gagcagttcg atcgtatctg tttcggccaa cgtgaaaccg cttttcaata
     9301 tgttgccctt catagtgaca ttgatagttg tgggaggttt cataggtaag acacgatctc
     9361 ctgcggcaga tggcgaacct tacgagaaag agtcataaaa aaggcccctt tcaggggcct
     9421 tttttatgga agtctaaggg cctgctggag acggtgcagg gtcttgtttc tgccgagcag
     9481 gaagacgaca tctacgagtt ccggtccctc ttcgctggca gtcaacacgt gtctcaggct
     9541 catgtagaag gacttcctgt cgggttttat ctcctttagt atctctttga tcgtcgagac
     9601 tatctcttcg ttgctccagc tgttcaggta ctctatcctg tccttgaaag cttcgagtga
     9661 tctgaagacc ttccgctgtt cctcgttctc ggggctcaga ggtctcacct ggatttcttc
     9721 gaagtagagc ttcatctttt caggtatttc cgacagtaat tccacaccct ttctcaccga
     9781 atcgatggcc cgctttagcc acttcaggtt cgcaccggcc tcctcgagcg agatcagccc
     9841 ggcctgcacg aggaagggta tggtgagatc ggtaattcta tccagatcgg cttcgcgtat
     9901 ataaaccccg ttcatccagc gagctttggc ttcatcgaat atagccgcac tggtgttgac
     9961 cctgtctatg gtgaaactct ctattatttc gccgtaggaa agtatctcct ttccctccgg
    10021 gtgcgaccat ccaagaaggg tgagaaagtt gaaaaaggct tccgggagaa aacctctgtc
    10081 cctgaattgt tcgaccgatg tgtctccatg cctcttgctg agttttttac cgtcgggacc
    10141 aaggatcatc gatacgtggg cgaacgaagg gatgggcgct tcaaaggcct cgtaaagcgc
    10201 gagctgccga agggtgtttg aaaggtgatc gtcccccctt attacgtgcg tgatctccat
    10261 caatatatca tcgacgacga ccgcgaagtt gtagatgggc tggttgttgg agcggagtat
    10321 cacgaaatcg ccgatcgcgc cttctttgaa aataacttcg ccctttatca ggtcgaggag
    10381 acggtactcc tttctcggca tcttgaaaaa aaccactggg ttgagacccc cgccctcgaa
    10441 ctcctttttc ctttcgggag tgttgaactt ctctagcatc tgctgatcgt agtggggcgg
    10501 cttgccctgg gagaggagat catcatgaag cgtctctatc tcttctgggt aggcatagac
    10561 tctgtaggct ttgccggaat ttatcaactc atcgaccatt ttcacgtata tatcggtcct
    10621 ctcggtctgt ctgtacggac ccgtctctcc cccgacatcc ggcccctcgt cccaggttat
    10681 tcccatccag aggagagatt ccatcagctg ctcttcgtac tctttcgtag atctggaaat
    10741 gtctgtgtct tctatcctga gcacgaattt gccgtcgtaa tgcttggcga aaaggtagtt
    10801 gaaaagcgcg gtacgcgcgc cgccaacgtg cagatagccg gtgggacttg gagcgaatct
    10861 caccctattc attttcatca tagactctcc tttctgcgca gggcaagtag aacaccggaa
    10921 attgtcttgc tatcgcttat gcttccttct agtatcctct ttaccacttc ttcgattctc
    10981 ataaagaccg gttcgacgaa ctcaccttcg tcgggatcgg attctccgac cctctcgact
    11041 tcgcaggtga aaatgtggat cgcctcgtcg gaaaagcccg gcgaagtctc tatcaccgtc
    11101 agtttttcaa gcctcaccgg tccgtaaccg gtctcttcga gcagttctct cctggcgcag
    11161 ttcacggggt cctctccttc atcgagtttg ccggccggta tttccagcat atacttctga
    11221 acaggaaacc tgtactgcct taccagcagc agttcttctc ccttgaaggc gacgactgcc
    11281 acggctccgg gatgccttac cacctctctg gaagcctcgc tgtcgtcgat caacttcacc
    11341 agatgtctct ccagtttcaa tatccttccg gagaagatct cctctttatg caacgaaatc
    11401 tccatttcat tcctccagtt cgaagtgcat ggggagcagc tcgaaaacag ttgttcttac
    11461 aacactgtcc ttccccacca gtatcacttc gaagtctccg aattcggcca tgaactgtct
    11521 gcacattcca cagggagaag tcgcttcatc tctcttgctg acgatggcta tagccctgaa
    11581 acttctccgc cccctggaca cggccgaggc tatcgcggat ctctcggcgc agatcgataa
    11641 accgaacgac gcgttctcga cgttcgtacc ggtgaagatc tccccttcca ccgtcagaag
    11701 ggccgctccc accggaaaat tcgaataggg agaataggag ttctccatgg cttcaatagc
    11761 tttctgtatc agtatgttct cctttgaact gtctttcatc gacgctcctc ctgtcaacgc
    11821 ttagcctgat cttttcgacc ttgttctttc cggccgccag gatctcgaag gtaaagccgt
    11881 tgactattat cttctctccc acttcgggaa atctttcgag aacctccaac aagtagccgc
    11941 cgatagtttc gaactcggta tcgggaaaga cctgttcgag ctccctctcg atatcgttta
    12001 taggtgtcat gccatcgact atgtactcgc tgtcgctgac cttttctatc atcatttctt
    12061 cggactcttc gtcgtactcg tccagtattt caccggtcaa ttcctctata gcgtcttcca
    12121 tggttatcag gccggccgtc cccccgtact cgtcgataac tatcgccata tggttcttct
    12181 tctctttgaa ctctctgaga aggtcatcga cctttttagt ttcgggaacg aagtagggcg
    12241 ccctcattat ttctttgaca tggacgacct ggagaagatc gttatctttc ctgtccagga
    12301 tgaagttgag cagatccttc gcataacaaa ccccgacgat cctgtcgaca ttttccctgt
    12361 atatgggata acgcgaataa ccttccgatt ctacgagctc catcaattct atgagcggtg
    12421 cgtcctcttc cagtgaaact atctcgaccc tcggagtcat tatctccttg accgagatat
    12481 ccttcaactc gagagtgcgc ttcatgatca ttctctcttc ggattgaaga acgccctctt
    12541 cgtgtccggc gtccacggca gatcttatct catcttccgt tatgaacgga gcgtacggga
    12601 ctttttttcc acccactatg actatgaaga gattcgagat gcgcagaagc aaccagagta
    12661 ggggcctgag aacagacgtt aagatggtta taaaaactat aactctcttg aaaaaacgct
    12721 cggggttctc tctggcaaag atcttgggtg ttatttcacc gaagaccagt atcagaaaag
    12781 tcatcacgcc ggttactatc gcagcggcga cacctgcaga gttctgcggc aacagctgga
    12841 ttgcaagaag cgtggcgagt gaagaggcga gaatgttcac tatgttgttc attaccagaa
    12901 tagctgtcag aatgctgttg ggattgtcaa tgaaattctt aaccgatttg ctg
//
LOCUS       DAOOLY010000043        12685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5479_length_12685_cov_5.292082, whole genome shotgun sequence.
ACCESSION   DAOOLY010000043 DAOOLY010000000
VERSION     DAOOLY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_12685_cov_5.292082"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1500
                     /locus_tag="PLD15_03640"
     CDS             19..1500
                     /locus_tag="PLD15_03640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPB63025.1"
                     /translation="MRKMLLGLFVFLVVVASGMTFEQKDISEFFDITMNDFLEKYEIP
                     GAVLSFVSDGEVLYVQGYGYSDLESKTPMKADSLVRIGSVSKLFTWISLMQLYELGEI
                     DLEANVNDYISEFKVPDTFPQPITIENLLTHTPGFEETPYNVIQFEEYTVQPLGEALK
                     WMPERAFEPGKFAAYSNYGTALAGYIVQEVSGVEFSDYVDMNIIEKLGMKSTTVKQEL
                     EPDLRARMSTGYYYLDGRNEPLTPFEKITIPPAGSITSSAGDMAKFIIANIQKGEFKG
                     ERILNEKTAELMQSVHFQHDPRLNGMCYGFYEMSMNGLRLIGHGGDTGLFHSLLVFST
                     EDNWGFFVSFNSNGKGSARDALLSAFMDRFYPVDQSIERKEVAYFDKIKITGDYISNR
                     RVFSDPFERLESWMNPDVTAEMSPDGDLIIQGLEYCFIDDNLAMAKNEKSLVAFRTEG
                     AKSYFFPNSAPILAFEKIPWYMSQKLVGSMIFVLLAGLFLLLP"
     gene            complement(1921..4059)
                     /locus_tag="PLD15_03645"
     CDS             complement(1921..4059)
                     /locus_tag="PLD15_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPB63026.1"
                     /translation="MSEMVLDIARSLGLPAWKVENAIELLEDGKTIPFIARYRKEKTG
                     ELNEIQLRDIADTLEYMKKLQARKEEVLRLIEEKGKLTDELKNRIVEAGTLQVVEDLY
                     LPYKSRKKTRADKAREKQLQSLADFLKTARKRDDGFIVGFVNAELEVATVEEALEGAR
                     DILAEEFAQDIVIRERVRSELKTKGIIKSEKTDAEDERSVYRDYYDFSQPINRLAAHR
                     VMALFRGEREEILKVHLELPFDILPALKRAAGWIDSLAYYSELEEAAIDSFSRLLFPS
                     IEREVRTQIREEAEERAIHVFARNLRDLFLQPPLGEKVVMGIDPGYRTGCKLAVIGRD
                     GALLYHDVIFPTPPQNDYAGSAMKVVQAANLLGVELISIGNGTGSRETEQFVSRLIKE
                     HGLAIKYMITTESGASVYSASKVAIEEFPDQDVTTRGAISIARRVQDPLAEYVKIPPE
                     AIGVGMYQHDVNQSELKKALDREVESVVNMVGVNLNTASEHLLKYISGLGPKVASSIV
                     KYRSENGPFKNRKDLMKVTGLGPKAFEQAAGFCRIVNGDEPFDGTTVHPESYAVAIEL
                     LDKIGVPSVELFEKRLELPERLAAIDVEDFSKEKDFNPITVREVIGMLKKPGLDPREE
                     LPQPLLRTDVLTMEDLASGMELEGTVRNVVDFGAFVDIGVKQDGLIHKSRMGNRVRDP
                     LEVLSVGQIVKVKIVEVDLQRMRIGLELLV"
     gene            complement(4052..4996)
                     /locus_tag="PLD15_03650"
     CDS             complement(4052..4996)
                     /locus_tag="PLD15_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB63027.1"
                     /translation="MTKFSIVATVKNRKLLKSVEMMQASAIRFNSSHCDLGELESFLE
                     YYNRNCSLPLYLDLQGAKFRLSRNQPEFEIKTGEQVVLGSSDSALKTISIDPRALSYL
                     SAGMRVSIEDGRIELEVLKAESEYARALVLRGGTIKAAKGMNISPHPVNQIELSSRDA
                     EIVTLSKKYGFIRYALSFVSSSCEVAELKELSQRPVASKIERELPFDRIEDIAKASDE
                     IWFCRGDLGAQLGTRGLVDFYRFFTRNIERLNRPVLMAGEVLEHMVDHPCATRSEICH
                     LVDIKESGYAGVVLSNETAYGSFPIETIKTVLEVTSDE"
     gene            complement(5026..6999)
                     /locus_tag="PLD15_03655"
     CDS             complement(5026..6999)
                     /locus_tag="PLD15_03655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPB63028.1"
                     /translation="MSQRYLTVEEQLSLPLVNGTVISPDGKKLAYTLRRTDWDGNKYM
                     RTVVVYDAESETALHVSNDEFDCSLPDWSPDSKRLVFLQKTGEENKNDLMIFDFELKR
                     SYKLLTFEQEISQMKWSQKNDGLFLVAASPESEELKKRKESYGEFEYIDEETKNSSLY
                     FVHLSRAMKKYSARFELPRDMRCEEALFDKLTDGKEFHISSISLSPDGNYIALLAPPT
                     SDYNDFDSAKVFLMNEERELKELPLAHPRGCLFSGDSKRLACVVPAGDDPWMDNGVVE
                     IVDLSDLSKRRLSVGQDLNIEPVAWTEKGFFVNWIEDCTIRLGLLDEEGHLEGLTDED
                     QMVMASSVTPDGKRLAIVMAGIFESPEIYLDGRKITDIASIHPRRERVRKERLSWKSC
                     DGTEIHGVLNLPTDFDSSRKYPLVVLVHGGPTWTALAARITSSYYPIEQLVSRGVLVL
                     EPNYRGSEGYGRDFRKLNYRNLGIGDYEDVVSGVDSLIERGLVNGDRLGVMGWSQGGY
                     ISAFCATYGKRFKAASVGAGISNWMTYHVNTDIHQFCHRYLGNNPWEDPQIYELTSPI
                     SYIKNASTPTLIQHGDSDTRVPTPNAFELYQGLREMNVPVRLVIYKDMGHNTVKPGLH
                     RAIMTQNLAWFSHYLLGEPLEGSFKMTDALSPG"
     gene            7239..7505
                     /locus_tag="PLD15_03660"
     CDS             7239..7505
                     /locus_tag="PLD15_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63029.1"
                     /translation="MISEEYTGNGHFEITRHYGYNFSGLFCEGGRPCRELFGLLFDGG
                     KVSIYTWIDGKSIDFNGEISKDNMSGTFIIHSTQEEGTWTASKQ"
     gene            complement(7558..8493)
                     /locus_tag="PLD15_03665"
     CDS             complement(7558..8493)
                     /locus_tag="PLD15_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPB63030.1"
                     /translation="MFLAVVSAVFFGWALGANDAANVYGTAVASGLVKFKVAVASSVI
                     FILIGAVFAGSRVLETISSLSSQTVFTAVTGTIAAALTMTIMTLLSIPVSSSQAMMGA
                     IFGVGMFQGTADWSIFLKIVVCWVVTPVGAAVGGFFLYRITSIFFAKIKTIPTQNLAL
                     KIGAIAIGAYGSFALGANDVANVIGPFAGIIPTNVAVLMGGISIGLGVITFGKRVMYT
                     VGKQIISLDHFAAVIAVLSMSSVVWMFTLLGIPASTSQAIVGAVIGAGFAGGTKDVNL
                     KILKKIALGWVQTPLVAGLLSFFLCLLLDFVIKIF"
     gene            8810..9313
                     /locus_tag="PLD15_03670"
     CDS             8810..9313
                     /locus_tag="PLD15_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63031.1"
                     /translation="MKNAILVLCLLLVTSLSFGSLSLGLINVAAQTESLSNTPLSGFV
                     GIDVLGIDLRYDFGPLSFGIATPFLMFTLSEDTGVKSSFMIPGIAWYGYVGLKLDLGA
                     FYVRGDIGHTFAFGEQSQLGFSPLRLGVGMDFSPGYYIELNVDLILQKINETVGKIFD
                     FKIGYRF"
     gene            9336..9836
                     /locus_tag="PLD15_03675"
     CDS             9336..9836
                     /locus_tag="PLD15_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63032.1"
                     /translation="MKRSLATAIVITVIAISCISRNPTVEAYRNSFYSVTFLDIESFS
                     VNLTTDKINISRDEKRMLNDGDILIYLTDEDRLGKMLILELDNKRSGILLFDFVTYDR
                     DGQILLEKKEVKLRASYIFDFDKGIIPEKIEGVELWWHNMDDMEMYLVPWTPTKLGKY
                     PLAKMN"
     gene            10375..10908
                     /locus_tag="PLD15_03680"
     CDS             10375..10908
                     /locus_tag="PLD15_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63033.1"
                     /translation="MKRFTFIMMLLSTLIILSGCILVLKCINFEGFAFGATYHFGDTL
                     WESATKMTLKEFYWSNGTPTNNGNLKVDNKGLAGHTGLDVNLNNITVAFTFPSAPTGL
                     ILYYGEYGGNINVEVNGDLKNVQNFADINGAVIGGVNVSITNVVGQKGVLNLLGTINS
                     FSIGGQELWIDHVCPRK"
     gene            complement(11058..12227)
                     /locus_tag="PLD15_03685"
     CDS             complement(11058..12227)
                     /locus_tag="PLD15_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPB63034.1"
                     /translation="MSETVHRIQLKDKEIILIGTAHVSKNSAEEVKRIIEEERPDSVA
                     IELCNSRYQSMQDQDRWKKTDIAKVIKDKKSFLLLANLILSSYQKRMAKQIGIQAGQE
                     MLQAIESARETGAQLVLADRDIQVTFARIWGNLSFWGKAKLFFSLVLSIFSDEKITEE
                     ELDKMKSGDMLTSALSELSKSFPQLKETLIDERDKYLAQKIKEAPGKKIVAVVGAGHV
                     PGLKEAIKEEQDLEALSRIPPKKKTGKIVGWTISIAIISLIVATVIVNRDAGLDQILS
                     WILWNGSLSALGTLAAFGHPLSILTAFAVAPISSLSPLLAAGWFAGLVEAMVRKPHVS
                     DFENLSEDVNSFKGFWRNRVTRILLVVVLANVGSTVGTFIGGADVVRIFIKSFWG"
     gene            12358..>12685
                     /locus_tag="PLD15_03690"
     CDS             12358..>12685
                     /locus_tag="PLD15_03690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63035.1"
                     /translation="MSDPVVARVEDIPAMIELSSQVFKTDMGSCFPLLFSPENVENMT
                     VVREAGRPVTMVGMLPREISVFGHSLKVGLIGAVCTHTDYRGRDYGAKALAMSEKMAI
                     EKGISLF"
BASE COUNT         3079 a         3184 c         3056 g         3366 t
ORIGIN      
        1 tttgctgggg ggtaaatgat gagaaaaatg ctgctaggat tgttcgtatt tctggtcgtt
       61 gttgcttctg gaatgacttt cgagcaaaag gatatttccg agttttttga catcacgatg
      121 aacgatttct tggagaagta tgagattccc ggtgccgtgc ttagtttcgt gagtgatgga
      181 gaggtcttat atgttcaggg ttacggttat tccgatcttg agagcaagac gccgatgaaa
      241 gccgactcgc tggtacggat cggttctgtg tccaagctct tcacgtggat ttcgctgatg
      301 cagctgtatg aactcggaga aatcgatctc gaagcgaatg tcaatgacta tatttccgag
      361 ttcaaagttc cagacacttt cccgcaaccg atcaccattg agaatctttt gactcataca
      421 ccgggctttg aagaaacgcc atacaacgta attcaatttg aagaatacac cgtgcaacca
      481 ctcggagaag ctctgaaatg gatgccggag cgagcatttg aaccgggaaa attcgcggcg
      541 tactcgaatt acggaacggc tctggcggga tatatcgttc aagaagtctc gggcgttgaa
      601 ttctccgact acgtggatat gaatataatc gagaagctcg gaatgaaaag cacaaccgta
      661 aagcaggaac tagaacccga tctcagggcg agaatgagca cgggttacta ctacttggat
      721 ggaaggaacg aacctctgac tcctttcgag aaaatcacca tcccgcccgc cggatcaatc
      781 acatcgagcg ccggcgatat ggcgaagttc ataatagcca atatccagaa gggggagttc
      841 aagggagaac gtatcctgaa cgaaaaaacc gccgaattaa tgcaaagtgt gcactttcag
      901 catgatccaa ggctgaacgg gatgtgttat ggcttctacg aaatgagtat gaacggactt
      961 cgcttgatcg ggcacggcgg tgacacgggt ctctttcact ctctcctggt gttctctact
     1021 gaagacaact gggggttttt cgtttccttc aactcaaatg gaaaaggttc tgcaagagat
     1081 gcattgctca gcgctttcat ggatagattc tatcccgtcg atcaatctat cgaaaggaag
     1141 gaagtcgctt acttcgataa gataaaaata accggtgatt atatatctaa cagaagagtc
     1201 ttctccgatc ctttcgaaag actcgagtcc tggatgaatc cggacgttac cgctgaaatg
     1261 tcgccagatg gagatcttat catacagggc ttagaatact gcttcatcga cgataacctt
     1321 gcgatggcaa agaatgaaaa aagcctcgtt gctttcagaa ccgagggtgc gaagagttat
     1381 tttttcccca attcagcacc gatcctcgca tttgaaaaaa taccctggta catgagccag
     1441 aaactggttg ggagcatgat ttttgtcctg ctggcagggc tgttcctttt gctcccctag
     1501 agtttttgga actcaattcg aacccagatg caagacctaa tacccgccct tcgaaaaaac
     1561 ggatgaaccg tccgctcttt cgaagggcgg gttctatatg tgtatgtttt cctcgccagg
     1621 agtttcgtaa ccgattcttt acatcaaatc ccgataatac tactcacata tattgacaaa
     1681 gccgagttaa ttgggtagaa ttatatcaag aagaacagga agacaagaag aacccttgca
     1741 acccccaaag ctcaaccggt cattaacaac aagacaaagc acctgagcac cgcgatatgc
     1801 acagtccacc attacccccc aggttctgga acagacggac aggcatgttg aaaccccctg
     1861 gaatatcccg attgatagcc gccgaaaggc ggcttttttt tattgtattg gcattgtttt
     1921 ctacaccagc agctcgagcc cgatccgcat tctctgcagg tcaacctcga caatcttcac
     1981 tttcactatc tggccgacac tgagtacctc tagaggatct ctgaccctgt tacccatcct
     2041 gcttttgtgg atgagaccgt cctgcttcac acctatatcg acgaaggctc cgaaatccac
     2101 cacgtttctc acagttccct ccagctccat tccagaggcg aggtcttcca tggtcagaac
     2161 gtcggttctc aaaagaggtt gaggaagctc ttccctgggg tccagtccgg gttttttgag
     2221 catgccgatg acctctctga ctgttatcgg attgaagtcc ttctctttcg aaaaatcttc
     2281 gacatctatt gccgcgagtc tttcgggtaa ctcaaggcgc ttttcgaaaa gctctacgga
     2341 agggacgccg atcttatcca gcagttctat ggcgaccgca taactttcgg ggtggacggt
     2401 tgttccgtcg aaaggctcat cgccgtttac gatccggcaa aaaccggccg cctgttcgaa
     2461 ggctttagga ccgagaccgg taactttcat tagatccttc ctgtttttaa aaggaccgtt
     2521 ttcggatctg tacttgacta tgctcgaggc gaccttcggg ccgagaccgg agatgtattt
     2581 cagtagatgc tccgaggccg tgttgagatt gacaccgacc atattcacga ccgactccac
     2641 ttctctgtcc agggctttct tgagttccga ttggttcaca tcgtgctggt acatgccgac
     2701 ccctatcgct tcgggcggta tcttcacata ctcggcaagc gggtcctgca ctcgcctggc
     2761 gatggatatc gcgccccggg tggtcacgtc ctgatcggga aattcttcga tggccacttt
     2821 cgaggcagaa taaacggagg ctcccgattc tgtggtaatc atgtatttta tggccagtcc
     2881 gtgttctttt atgagtctgc tcacaaactg ctccgtctct cttgaacccg ttccgtttcc
     2941 tatggatatg agctccaccc cgagaaggtt ggccgcctga acgaccttca tggccgagcc
     3001 ggcgtaatcg ttctgcggcg gagtcggaaa aatcacatcg tggtagagca gcgcgccatc
     3061 cctgcctata acggccagct tacagccggt gcggtaaccg gggtcgattc ccataacgac
     3121 tttttctccc aggggaggtt gaagaaagag atctctgagg tttctggcga agacgtgtat
     3181 ggccctttct tcggcttctt cacgtatctg tgtccttact tctctctcta tcgaaggaaa
     3241 gaggagccgt gagaaggaat cgatggccgc ctcttccagt tcgctgtaat aggccagaga
     3301 gtcgatccag cccgccgctc tcttcagagc cggcagtatg tcgaaaggaa gctcgagatg
     3361 cactttgaga atctcttcgc gttcgcctct gaagagcgcc ataaccctgt gtgccgccag
     3421 acgatttatc ggctgggaaa agtcgtagta atccctgtac accgagcgtt cgtcttccgc
     3481 gtcggtcttt tcgctcttta taatcccttt ggttttcagt tcgctcctga ctcgctccct
     3541 tatcactata tcctgggcga attcctcggc caggatatcg cgcgcgcctt ccagggcctc
     3601 ttcgaccgtc gccacttcga gttcggcgtt tacgaaaccg actatgaagc cgtcgtctct
     3661 tttcctcgcg gttttgagga agtcggccag cgattggagt tgcttttccc tggctttgtc
     3721 ggccctggtt tttttcctgg acttgtaagg caggtagaga tcttcgacga cctggagagt
     3781 gccggcctcc actattctgt ttttgagttc gtcggtaagc tttcccttct cctctatcag
     3841 gcgaaggacc tcttcctttc tggcctgaag ctttttcata tactccagcg tgtcggctat
     3901 gtctcttagc tgtatctcgt tgagttcgcc cgtcttttcc tttctgtatc tggctatgaa
     3961 agggatggtc ttcccgtctt caagaagctc gatggcgttc tctactttcc aggcgggaag
     4021 accgagcgat cgtgctatat ccagaaccat ttcactcatc ggaagtaacc tccagaactg
     4081 tctttatagt ctcgatcgga aaggagccgt aagcggtctc gttggagaga acaactccgg
     4141 cataaccact ctccttaata tctacgaggt gacagatctc gctccgcgtg gcgcagggat
     4201 gatccaccat gtgctcgagc acctcgccgg ccatcaggac cggtctgttg agtctttcga
     4261 tatttctggt gaagaacctg tagaaatcaa ccaaccccct cgtaccgagc tgggccccga
     4321 gatctcccct gcaaaaccag atttcgtcgc tggcctttgc tatatcctca atcctgtcga
     4381 aagggagctc gcgctctatc tttgaagcga caggtctttg agacagttcc ttgagctcgg
     4441 caacctcgca tgatgaagag acgaaggaca gtgcgtacct tataaagccg tactttttgc
     4501 tgagcgttac tatctctgca tctcttgatg aaagctctat ctggttgaca ggatggggag
     4561 atatattcat tcctttggca gccttgattg taccgcctct cagaaccagc gccctcgcgt
     4621 attcggactc tgccttcagt acctctagct cgattctgcc gtcttctatc gagaccctca
     4681 tccccgcaga aagatacgac agggctcgag ggtctatcga aatcgttttc agtgccgaat
     4741 ccgagcttcc taaaaccacc tgctcgcccg tcttgatctc gaactccggc tgattgcgcg
     4801 aaagtctgaa ctttgcgccc tgaaggtcta gataaagagg aagcgaacag ttgcggttgt
     4861 agtactcaag aaaggattcg agttcaccca gatcgcaatg ggaagagttg aacctaattg
     4921 cactggcctg catcatttcg acggatttga ggagcttcct gttcttcacg gtagccacga
     4981 tagagaattt ggtcatatcg atcacctcta gaaagattat aacagtcaac ctggagaaag
     5041 agcgtctgtc atcttgaagg atccctccag cggttcgccc aggagataat gactgaacca
     5101 ggcgaggttt tgtgtcatta tggccctgtg aagaccgggt ttaacggtgt tgtgtcccat
     5161 gtctttgtag ataaccagtc ttactggcac attcatctct ctcagaccct ggtaaagctc
     5221 gaaggcgtta ggcgtcggaa ctctggtgtc ggaatcgccg tgctgtatga gcgtgggggt
     5281 agaagcgttc ttaatgtaag aaatgggcga tgtcagctcg tatatctgag gatcttccca
     5341 ggggttgttg ccgaggtagc gatggcagaa ttgatgaatg tcggtgttga cgtggtaggt
     5401 catccagttg gatatacccg ctcccacgga ggcggctttg aatctctttc cataagttgc
     5461 gcagaaggcc gagatgtagc ctccctggct ccagcccata actccgagac ggtctccgtt
     5521 caccagccct ctttctatga gcgaatcgac gccgctcaca acgtcttcgt aatcacctat
     5581 cccgaggttc ctgtaattca atttcctgaa gtctcttccg tagccttcgc ttccgcggta
     5641 gttgggctcc agtaccagca ctcctctgga taccagctgc tcgataggat aatagctgct
     5701 cgttatcctc gcggcaagag ccgtccaggt cggtcctccg tgaaccagca caacgagagg
     5761 gtatttcctg gaactgtcga aatcggtggg aaggttcaaa acaccgtgga tctcagtacc
     5821 gtcgcaactc ttccaggaga gtctttcctt ccgtaccctt tcccttcggg gatgtataga
     5881 cgcgatatct gttatttttc ttccgtcgag atagatctcg ggagattcaa aaatgccggc
     5941 catgacgatg gccagtcgtt ttccatccgg agtgacgctt gaggccatca ccatctgatc
     6001 ctcatccgtc aggccttcca gatggccttc ctcatcgagc agtcccaggc gaatcgtaca
     6061 gtcctcgatc cagttcacga agaaaccctt ctccgtccag gcaaccggtt cgatgttaag
     6121 gtcctgtccc acactcaacc gccttttcga aagatcggaa agatctacga tctcgaccac
     6181 tccgttatcc atccaggggt cgtctccggc cggtaccaca caggccagtc tcttcgagtc
     6241 gccggagaag agacagcccc tcgggtgagc cagcggtagc tctttcagtt ctctttcttc
     6301 gttcatgaga aaaaccttcg cgctatcgaa atcgttgtaa tcgctcgtgg gtggtgccag
     6361 aagtgcgatg taatttccgt cgggagatag agagatcgaa gagatatgaa actccttgcc
     6421 gtccgtcaac ttatcgaaga gagcttcttc gcagcgcata tctctgggta attcgaacct
     6481 tgccgaatat tttttcatgg cgcgcgagag atggacgaaa tacagcgagc tgttcttcgt
     6541 ctcttcgtct atgtattcga actctccgta gctttccttt ctctttttca gttcttcgct
     6601 ctccggagag gcggctacga gaaagagtcc gtcatttttc tgactccact tcatctgact
     6661 gatctcctgt tcgaaggtga gtaacttgta ggaacgtttc agttcgaaat cgaagatcat
     6721 cagatcgttc ttgttttcct caccggtttt ctggagaaag acaagcctct tcgaatcggg
     6781 cgaccaatcg ggaagagagc aatcgaactc atcgttcgag acgtgtaacg ccgtctcgct
     6841 ttccgcatcg taaaccacaa ccgttcgcat gtacttgttt ccgtcccaat cggttcgcct
     6901 gagcgtgtag gccagctttt tgccgtcggg tgaaatcacc gtaccgttga ccagaggaag
     6961 cgagagctgc tcttcgactg tcaggtatct ctggctcaaa tcatatccct cccaaaataa
     7021 aagtgccaat atagtataac ccgcaccgaa ggagtaaaac aaaccgcgta atttgcgaag
     7081 atccagacct gtttgtggta taaacttatc agggtggtgg gatgatgatt aagagacgct
     7141 tatcgattct tggacttctc tctttggttc tgtgcctgtt tctttctgga tgcctatcac
     7201 ggaagtttcc agtgatgtcc ggcctgtggg atctagagat gatatcggag gaatataccg
     7261 gtaacggtca tttcgaaata accaggcatt acggctacaa tttcagcggt cttttttgtg
     7321 agggcggtcg tccgtgccgg gagcttttcg gattgctctt cgacggtgga aaagtttcta
     7381 tctacacatg gatcgacggt aaaagcatcg atttcaacgg cgagatctcg aaagacaaca
     7441 tgtcgggaac cttcataatt cactccacgc aggaggaagg cacctggaca gcatcaaagc
     7501 aatgagcgag tccctttctt ttgaaaaatc cagtcggtca gaaaggctag ctcgctatta
     7561 gaagatcttg ataacaaaat caagcagaag acagaggaaa aaggataaga gtccggcaac
     7621 gagcggggtc tgtacccatc ccagcgctat tttcttcaga atcttcagat tcacgtcttt
     7681 cgtgcctccg gcgaatcccg ctccgatgac agccccgact atcgcctgag aggtggaagc
     7741 cggtatgccc agcagggtga acatccacac gaccgacgac atggaaagca cggcgattac
     7801 ggcggcgaag tgatcgaggc tgatgatctg ttttcccacc gtgtacatga cgcgcttgcc
     7861 gaaagttatc acgccaagac ctatgcttat gcctcccatc agcacggcga cattggtcgg
     7921 aattattcct gcgaaaggac cgataacgtt cgctacgtca tttgccccga gcgcgaagga
     7981 accgtacgct cctatggcga tggcaccgat ttttaaagcg agattctgtg tcggtatggt
     8041 ctttatcttt gcaaagaaga tcgatgttat tcggtacaga aagaagccgc ccacggcggc
     8101 cccgacagga gtgaccaccc agcaaaccac gattttcaga aatattgacc agtcggccgt
     8161 tccctggaac attccgacac cgaagatagc tcccatcata gcctgtgaag aggagactgg
     8221 aatgctcaga agagtcatga tcgtcatcgt cagagccgct gcaatagttc cggtgacggc
     8281 tgtaaagacg gtttgcgaac tgaggctgga gatagtctca agaactctag agccggcgaa
     8341 gaccgcgcct atgagtatga atatcacgga tgaagcgacc gcaaccttga acttaacaag
     8401 acccgatgcc acggccgtac cataaacgtt ggctgcgtcg ttcgccccca gtgcccatcc
     8461 gaaaaatacc gccgaaacta ctgcaagaaa cacatcaagc ctccagataa gggaaaatac
     8521 gatctccaaa caatcctata attaagatat taggtgatct ttatgcgaat gttttcccag
     8581 ttcgcattgg agatatgttt ctaatatgca agctaaactt atcaatcgaa gataaaacca
     8641 gtcgatattt agatccaaga agccttctct ggaaaatgga aggagtcccc gggtagattg
     8701 acgttccgtg agacctgcac agaattctct ggcaatggaa ctaacagtct gaaaaaccag
     8761 atcgtttttt gtattgtgga cttacaatga acattagggg gtgagaccta tgaagaatgc
     8821 catccttgta ctttgccttt tgctggttac ttcactttcg tttggaagcc tttctctcgg
     8881 attaatcaat gttgccgcgc aaaccgaatc tctttcgaat actcctcttt ccggatttgt
     8941 tggaatagac gtgcttggaa ttgacttgcg gtatgatttt ggacctctgt cttttggaat
     9001 agcaactccg tttcttatgt ttactctttc ggaagacacc ggagtaaagt cttcattcat
     9061 gattcccgga attgcctggt acggttatgt tggactgaaa ctcgatctcg gcgcttttta
     9121 tgttagagga gatatcggcc acacgttcgc ttttggagag cagtcacagc tgggtttttc
     9181 gcctctcaga cttggagttg ggatggattt tagtcccggt tattacatag agctcaacgt
     9241 agatcttatt ctacaaaaaa tcaatgagac tgtcggtaag atcttcgatt ttaaaatagg
     9301 ctacagattc taagagaaaa aaggggtgtt tgaagatgaa gagatctctt gccaccgcca
     9361 ttgtgataac tgtgattgca atctcttgca tttcgagaaa tccaacggtt gaggcgtaca
     9421 gaaatagttt ctactcagtt acttttctgg atatagagag cttcagtgtg aatctgacaa
     9481 cggataagat caatatctcg agagatgaaa aacgaatgct caacgatggt gacattctta
     9541 tctaccttac agatgaagac aggcttggaa agatgctgat actggagctc gacaataaaa
     9601 gaagcggcat actcttgttt gattttgtca cgtacgatag ggatggtcaa atcctccttg
     9661 agaagaaaga agtcaagctt cgggcttctt atatctttga tttcgacaaa ggaataattc
     9721 ccgaaaaaat cgagggtgtc gagctgtggt ggcacaatat ggatgatatg gaaatgtatc
     9781 tcgttccctg gactcccaca aaactcggga aatatccact ggcgaagatg aactgagaga
     9841 ccaacacatt tctcagaaga aatatgtttg accgattaga agaatctttt tagaatcaag
     9901 cttagcaaag aacatcggca taagcttcat gatggcatct acgaaaaact cgccaggcag
     9961 gctgatggga agccccggct ctccacaagt aaaaatctac cggaatcact atctcaactc
    10021 atgcgctttt gtgatccttc ttgcaagtaa agaaagcaac ctttgtccaa ccatttcgat
    10081 tcaccgctgg ataatcattt ctgcccttat tgtgttttta aatgtccacc aggtatattt
    10141 gtcgattttc aatggatgaa attgatggga aaaatcagcg tgaataccga tatagatatt
    10201 aattatgcgg gagttccgga gcatttatat ccattaaact taaccttaag cgagtttcga
    10261 aagttgttcg tttttctgcc gcaagtgcta gacttttcat gcgccgcgac gacatatggg
    10321 cgcagatcgg ttggtagttt gtccggtcct tatcttctgg ataggaggtg tatcatgaaa
    10381 cggttcacgt ttattatgat gctcctgtca accctcatta tcctctctgg atgcatcctg
    10441 gttctgaagt gtataaattt cgaaggtttt gctttcggtg ccacttatca tttcggagat
    10501 acactgtggg aatcggccac caagatgacg ctaaaggagt tctactggtc taacggcaca
    10561 ccaacgaaca acgggaattt gaaagtagat aacaaaggac ttgccggtca taccggcctc
    10621 gacgttaatc taaacaacat cacagtagcc tttaccttcc catcagcacc caccggtctc
    10681 attctctact acggcgaata cggcggaaat atcaacgtcg aggttaacgg agatctgaag
    10741 aacgttcaga atttcgccga cataaacggt gccgttattg gcggagtcaa tgtctcaatc
    10801 acaaacgtcg taggccagaa aggcgttctg aaccttctgg gaacgatcaa ttcattttcg
    10861 atcggcggcc aggaactgtg gatcgaccac gtctgcccga gaaaatgaag agatcatttt
    10921 tcacaggaat cagagcagca actcaagttg tcaagtgctg agttgagctt tcactaccct
    10981 atgtacgcat gtgaccgcct tcgttaagcg acaccggcaa aatggagtca tacttgagac
    11041 ggctttcagc gtaaaactca tccccagaag gatttgatga atattctcac tacatcggcc
    11101 ccacctatga aagttccgac agtgcttccg acgttcgcca gaaccactac cagcagtatc
    11161 cgtgtgaccc ggtttctcca gaagcctttg aaggagttca catcctcgct caggttttca
    11221 aagtccgaga catggggctt tctcaccatg gcttcaacga gaccggcgaa ccagccggcg
    11281 gcgagcaacg gactcaaaga actgatcgga gcgaccgcaa aggctgtcag tatcgatagc
    11341 ggatgcccga atgcggccag tgttcccagc gccgagaggg agccgttcca aagtatccag
    11401 ctcagtatct ggtcgaggcc ggcgtcgcgg ttaacgatta cagtcgccac tatcagcgag
    11461 attatcgcta tcgatatcgt ccagccgaca atcttgccgg tctttttttt gggcgggatc
    11521 ctgctcaagg cctccagatc ctgctcttcc ttgatagcct ctttaaggcc cggaacgtgc
    11581 ccggcgccaa ccactgcgac tatcttcttt cccggggctt ctttgatctt ctgggcgagg
    11641 tatttgtccc tttcgtctat cagagtctcc tttaactgtg gaaaggattt ggacagctcg
    11701 ctcagggccg acgtcagcat gtcgcccgat ttcattttgt ccagctcctc ctcggttatc
    11761 ttctcatcgc tgaagatcga gagaacgaga ctgaaaaaga gttttgcctt tccccagaaa
    11821 ctcaggtttc cccagatcct cgcgaaggtc acctgtatat ccctgtcggc cagcaccagc
    11881 tgcgcgccgg tctcccttgc cgattctatc gcctggagca tctcctgccc ggcctgtatc
    11941 ccgatctgct tggccattct cttctggtaa gaggagagga taagattggc cagcaacaaa
    12001 aaggacttct tatcctttat cactttggca atatccgtct ttttccagcg atcctggtcc
    12061 tgcatcgact gatagcgcga gttgcaaagc tcgatcgcca cggaatctgg tctctcttcc
    12121 tctataatcc gcttcacttc ttcggcgctg tttttagaca cgtgagcggt tccgatcagt
    12181 attatttctt tatctttgag ctgtattctg tgtaccgtct ctgacaaatc aatcaccggc
    12241 cctttctata ttctttcaaa gactatttaa tcacaatatt gcgtccggct ctcaattgtg
    12301 agattatcaa ttgtgtggtt tttgataatc gatatcgtgc tgagtgaggt gatcgccttg
    12361 tcggatcccg ttgtcgcaag agtagaagat atccccgcca tgatagaact ttctagccag
    12421 gtcttcaaaa cagatatggg cagttgcttt ccgctgcttt tttcaccgga gaacgtggaa
    12481 aacatgacgg tcgtgagaga ggccggaagg cccgtgacca tggtaggtat gcttcccaga
    12541 gaaatctctg ttttcggtca cagtttgaag gtcggtctca tcggcgccgt ctgcacgcac
    12601 accgattaca ggggcagaga ctacggggcg aaggccctgg cgatgtccga gaaaatggcg
    12661 atcgagaagg gcatatcgct cttta
//
LOCUS       DAOOLY010000044        12078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5860_length_12078_cov_5.063545, whole genome shotgun sequence.
ACCESSION   DAOOLY010000044 DAOOLY010000000
VERSION     DAOOLY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12078
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5860_length_12078_cov_5.063545"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..961
                     /locus_tag="PLD15_03695"
     CDS             89..961
                     /locus_tag="PLD15_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB63036.1"
                     /translation="MKKSIISVITLLSASLFAQGVPELYSAPVKKAQVGAIEMAYKVF
                     GSGAPLLMIAGYGSPLESWDPYVVSALSKEFTVIVFDNRGIGRSTTSDEAPSIELFAK
                     DAADLVRTIGFEKVSLVGWSMGGYIAQEFALNYPELTDKLVLLSTSCGGDESVPPSDE
                     AVSMLQNMGDPQVLLKVLFPADWTLAHPEVAARFTAASGQDGAGDQNQRGIADQYVAV
                     GNWSGTCERLKSIESDTMIIAGTEDIILPVKNSLILAEAIEGSWLVRLKDGGHGAIYQ
                     FPERIADLLITFLK"
     gene            1142..1798
                     /locus_tag="PLD15_03700"
     CDS             1142..1798
                     /locus_tag="PLD15_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB63037.1"
                     /translation="MVIKRVIAIPKETFFNLPLEKRERIIEAAIDEFSRNDYREAKIN
                     EIVRLSSIPKGSFYQYFEDKKDLFKYVIDLLYEKKMEAISNVMNSAVNTSAFQVLRLM
                     AEAAIEMAEENPRLSKIGDRLMADPALMREVFEDYRPSSDGLMEGLVRQGIERGDIAP
                     WVDPSLAARLITAFLLALGDAVRESGSGNLTEEARKKFYSMVDILEKGMRRREQDDRS
                     "
     gene            1785..2639
                     /locus_tag="PLD15_03705"
     CDS             1785..2639
                     /locus_tag="PLD15_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63038.1"
                     /translation="MIEVEGLYHSYTNDERYAVKGVSFNIKEGEIFGFLGPNGAGKST
                     TQKVLTGLLPLQRGSVKIAGKDAGKPDSSFYNMIGVSFEQPNLYRKLTGLENLDYFAK
                     MYDVPTEDPMELLRMVGLEEAASKRAGEYSKGMQQRLVFARSLINKPKIWFLDEPVSG
                     LDPTTSATIKEIIKRKKSEGVTVFLTTHNMYIAEELCDRVAFIVAGEISLIDSPRNLK
                     LQYGQKLVEVEYRLDGSVKKESFSLLQGSDKAKLNTLIEKGTIETMHSKEATLEEIFI
                     GVTGRELR"
     gene            2639..3679
                     /locus_tag="PLD15_03710"
     CDS             2639..3679
                     /locus_tag="PLD15_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63039.1"
                     /translation="MRLAANTLKELRLGWRSYYFLLVVGVAIAYFLLVTFLIPEDLTV
                     SPDVVVLNEMEEGSQVMENLFGGDMESDKLLLAKDRDDLEKTMNRRFNSVGIVLKGSI
                     REPGFELVFQGHESQDIRELLRLSILQRTGTLRSEEGYNIEYLEDRKPVDEAIPFNKS
                     FLPVLLMSEAVMLGMFLVFAMIFGEKSEETIKAYSITPGRIWEYLGAKVIVLAILGVL
                     FTLILTPMVVGIGPRYGELLLIVVVGSTLSTSIALIFASFYNNLSQSLVPMLGLSLVF
                     GLPMISFFIPGFSPWYLRILPTYTILFALKGAVFPGSIQVGISSLWLIALEGAIAFTI
                     AVLVYGQRVRKN"
     gene            3694..4704
                     /locus_tag="PLD15_03715"
     CDS             3694..4704
                     /locus_tag="PLD15_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63040.1"
                     /translation="MNVRRVLNIFRLDTLNSRRDKVMIYTLLAPIILTIVFKLISPDF
                     QSLSIDFIVPESEAELAVQLDRYANVEKVAGIREMKDAVARGGDRIGVYESGGKYVLV
                     MQGDESPQARELAELAISAVLQGDTSLINIDFSDNGRVIPPVTLFGFSFVVILSFTLG
                     GFIIGFNIIEEKESGAMRALMVTPVSKAEFLLGRSIMGISVPIIHALLAVLIFAIGGL
                     DILKLIMVTVVSSVIGIVMGFLIGVMSSSQMTGLANMKISVLLLLMPVMIAFVLPENR
                     QFIFYWSPTYWSFAALKDILTRSAEWGGFLLQLLWISLTTGLLFLFMRGKIKSGLKTY
                     QS"
     gene            5039..6202
                     /locus_tag="PLD15_03720"
     CDS             5039..6202
                     /locus_tag="PLD15_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB63041.1"
                     /translation="MIEGKEIASAGQLRYGMLGGGEGSFIGDVHRKAIAMDGMAELVA
                     GCFSIDYEDTLKTAKKLRVADDRVYKDYVEMARKESGREDCIDFVDIATPNNTHYPIA
                     KEFLNAGINVVCEKPLTIDLAQAGELAALAKKKELLFAVTYTYSGYVMVKHAREMIKR
                     GDIGEIRVVMGEYAQDWLATRLEDTGNMQASWRTDPSQTGRSNCVGDIGSHIENTVSY
                     MTGLKIKSLCSSLDSFVPGRKLDDNAEILVRFDNGARGVYWASQVAVGHDNGLRVRIF
                     GSKGSIEWEQENPNYLRVAYIGGPVQILSRGGSYMLPNAAKTSRIPAGHPEGYYEAFA
                     NIYRNFATALMKKKKGEDISDADFPSVEDGLEGVKFISRAVESSEAGSVWVDM"
     gene            6214..7206
                     /locus_tag="PLD15_03725"
     CDS             6214..7206
                     /locus_tag="PLD15_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPB63042.1"
                     /translation="MGKRPVTLFTGQWADLTFEEICKKASSWGYDGLEIACWGDHMDL
                     ERAATDPAYVENRKSILEKYDLKCWALGAHLEGQCVGDLYDSRLDGFAPDSCKGDPEK
                     IRAWGIKQMTFAPTAARNMGCYTVTGFTGSPVWKFWYSFPQTTEKMVEDGYKEIFDLW
                     TPIFDRFDAEGVTFSLEVHPTEIAFDYYTAKKLLEVFKYRKTLGFNFDPSHLIWQGVK
                     PHLFIRDFGDRIYHVHMKDAAVTLDGRAGILGSMLTFGDNRRGWNFRSLGHGHVDFEE
                     IIRELNNIGYSGPLSVEWEDSGMEREYGAREAAQFVKKVDFEPSNIAFDDALRS"
     gene            7218..8711
                     /locus_tag="PLD15_03730"
     CDS             7218..8711
                     /locus_tag="PLD15_03730"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB63043.1"
                     /translation="MGEVLIKTSGIAKEFSGVRVLDDINIEIEKGEIFGIIGENGAGK
                     STLVKIFSGIYNPTKGKLFFEGREVEIKDPIHAKAIGISLIPQEFNLINELNVYENIF
                     LGSELTGKGFLAKKDMKKRTRELLKELETTINPDEKIERLSVAQKQMVEIAKALAHDS
                     KLLIMDEPTTVLTQNEIKILFDLMRRLKGRGVTIIYISHKLKEVKSICDRVMVLRDGI
                     MISVTGTVELDEHEMAKRMVGRELSQVFPEKTEPSEEVVLEVKNLSVRGLLKDISFDL
                     RRGEILGFAGLVGAGRTELAEAIIGIRKKEAGAMKVKGRTVEIKSPNDAVKNKIAYLS
                     EDRQGSGILISFDIIGNTTLISLPAYSRILINHRLEREKTQKYVDKFNIRAASLKTRL
                     EFFSGGNQQKVSLAKSLDPEPEIFIFDEPTRGIDVNAKREIYWFINELVKEGVSCIII
                     SSELEEIIGMSNRVVVMKEGTLTGILEGEHINEEEIMLHATGLKGVA"
     gene            8713..9687
                     /locus_tag="PLD15_03735"
     CDS             8713..9687
                     /locus_tag="PLD15_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63044.1"
                     /translation="MAVETSRKRFEYERFGPLIALAILFIISAIASPYFLQVQNLLNI
                     MRQVSYTGIIALGMTFVIISGGIDLSVGSMVALVGGVVILTLNWAMGVFGAAAEAWAI
                     LFALVVGILFGAGLGALNGLMITLGKIAPFIATLGTMAIFRSMALYIGSAGVFRSQSN
                     LFPELGMGKILEIPNPVVVFIGLAIIFSVILKQTRYGRYLCAVGSNPKVAKYSAIKVN
                     LTRFISYVTVGITVGFSAVFLSARLNSMSSTTGGSNYELDAIAAVIIGGTPMTGGTGS
                     IFGTVIGAITLGIINNMLNMLGVSPYLQGTVKGIVIIGAVLIQRKRSQ"
     gene            9766..10728
                     /locus_tag="PLD15_03740"
     CDS             9766..10728
                     /locus_tag="PLD15_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63045.1"
                     /translation="MKKALVLTILVVFLLSAFAFAAEKIKIGVAIPSADHGWTGGIVW
                     WSQKAIKDWNAKDPDVEFFLVTADSAAKQVGDVEDLMVKGIDALVILAHDSEPLTPVV
                     EKAYNSGIFVVSVDRGLTKPVENVYVAGDNPGLGRVSGEWLAKALNYKGDIVVLEGIP
                     CVINSERVDAFNAVIAKYPDIKILDSQPAYWQTEKGLEIMENYLQKYKKIDAVWAQDD
                     DVLKGVLQAYKESGRNDIKIFLGGAGSKEMVKKVMDNDPLVKADVTYPPSMIATGISM
                     AVMAMRGEQLNGFYQQQIPSKIILAAELIVPENAANYYEPDSIF"
     gene            10914..>12078
                     /locus_tag="PLD15_03745"
     CDS             10914..>12078
                     /locus_tag="PLD15_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HPB63046.1"
                     /translation="MKKLSVVAFLLLMVAPLAANGVVVPPHPVEPGTGGFKMNYHNVD
                     ITLENGIARVKIEEEFENVSNRRLEAVYLFPIPSSAVISDFVMKVGDQVFKGEVLPAD
                     EARKIYQEIVAKLKDPALLEYVGSQLIRLSVFPFEPGEKRQFHIEYTQPLEISSNAYT
                     MIYPLKIDSFLTAPIGKVSIRIKTTDGIAEVFSPTHALKEVQASGREYIFQAEDFVPS
                     SDVAMIFTASTEEIPSSVATFWDEKAGEGYFLLTLIPRLKEERIIPKDVVFVLDISGS
                     MYGEKIEQAKRALEQVLQMLRSDDRFAIVTFDDGIYDLTGGLLSASEKGVWIEKVRRI
                     QADGMTNIYGALQTSIDMFSRGDEGRFKVLLFLTDGAPTSGITETGKIVGDATN"
BASE COUNT         3133 a         2836 c         3365 g         2744 t
ORIGIN      
        1 gtagaaagat catcatcgct attatgcgaa aggagggaag cgtcattcct caccgggcta
       61 aagcacggtg atgaatgaca ctgtttagat gaagaagtcg attatttccg taattactct
      121 tctgtcggca tcgcttttcg ctcagggcgt tccggagttg tattccgcac ccgtcaagaa
      181 agcgcaggta ggcgccatag aaatggctta caaggtcttt ggaagcggcg cccctctgct
      241 tatgatcgca ggttacggat cgcctctgga gagctgggac ccgtacgtag tttccgcgct
      301 ctctaaagag ttcaccgtga tagtcttcga taacaggggc atcggaagat cgacaacttc
      361 tgacgaggcc ccttcgatag agttgtttgc gaaagacgcc gctgatcttg tcagaaccat
      421 aggctttgaa aaggtcagcc tcgtgggatg gtccatggga ggttacatcg cgcaggagtt
      481 cgccctcaat tatccggagc tgaccgacaa actggttctt ctctcgacta gctgcggagg
      541 agacgagtcg gtgcctcctt cggatgaagc ggtttccatg cttcagaaca tgggggatcc
      601 acaggtgctt ctgaaagtgc tcttccctgc cgactggacg cttgcccatc cggaggtggc
      661 ggcgaggttt acggccgctt ccggtcagga tggagccggc gaccaaaacc aaagaggtat
      721 agccgatcaa tacgttgccg tgggtaactg gagtgggacc tgcgagaggc tgaaatcgat
      781 cgagtccgac accatgataa tagcgggaac ggaggatatc atactccccg tgaagaactc
      841 gctgatcctc gccgaagcga tagagggctc atggctcgtg agattgaaag acggcggaca
      901 cggtgcgatt tatcagttcc ccgaaagaat cgccgatctg ctgattacct ttttgaagta
      961 acccgttcac cgacaggctc ccgaaatctc tgcctcaaaa aacagtcagg tctcgatgtc
     1021 atgcggatcg tggatagctt tcgctcatct cttcatatga tcttaacatg accgatcggt
     1081 catgtacatt gaccgcccgt tctttttgat gtaatatatg accgagaagt catatgacca
     1141 agtggtcata aagagggtga tcgcgatacc aaaagagacg tttttcaatc taccgctcga
     1201 gaagagagaa aggatcatcg aagcggcgat agacgagttt tcaagaaacg actaccgcga
     1261 ggcgaagatt aacgagatag tcaggctgtc ttctataccc aagggtagtt tctatcagta
     1321 ctttgaagat aagaaggatc tgttcaagta tgtgatcgat ctgctctacg agaaaaagat
     1381 ggaggcgata tcaaacgtta tgaactcggc cgtgaacacg agtgcttttc aggtgctcag
     1441 gttaatggct gaggcggcga tcgagatggc cgaggagaat ccaaggttat cgaagatagg
     1501 agacagactc atggccgatc cggctcttat gagagaagtt ttcgaagact acaggccatc
     1561 gagtgacggg ctgatggaag gcctggtgag acagggcata gagaggggcg atatcgcgcc
     1621 ctgggtagat ccctcgctgg cggccaggct gattacggcc tttttactcg cgctcggaga
     1681 tgcggtcaga gaatcgggat ccggcaatct gacggaagag gcgagaaaga agttctactc
     1741 gatggtcgat attcttgaaa aaggaatgag gaggagagaa caagatgata gaagttgaag
     1801 gactgtacca ttcttacacg aacgatgaaa gatatgccgt caagggtgtg tcgttcaata
     1861 taaaagaagg cgagatcttc ggctttctgg gaccgaacgg cgcgggcaaa tcgaccacgc
     1921 agaaggttct gacgggattg ctcccactgc aacggggctc tgtgaagata gccggtaagg
     1981 atgcgggtaa acccgattcg agcttttaca atatgatagg cgtttccttt gagcaaccta
     2041 acctgtacag gaaactgacg ggactggaga acctggacta cttcgcgaag atgtacgatg
     2101 ttccaactga agatcccatg gaactgctga ggatggtcgg tctagaagag gccgcttcga
     2161 aaagggcggg agagtactcc aagggtatgc agcagagact ggttttcgcc cgctccctga
     2221 taaacaaacc gaagatctgg ttcctagacg agccggtttc ggggctagat ccgaccactt
     2281 cggcaacgat caaagaaata atcaaacgaa agaagagcga gggcgtgacc gtatttctca
     2341 cgacccacaa catgtatata gccgaagagc tctgcgaccg cgtagccttc atagtcgccg
     2401 gagagatatc gctcatcgat tctccgcgca accttaagct gcaatacggg caaaagctgg
     2461 tcgaagtcga atacagactc gacgggagcg tcaagaaaga gagtttttct ctgcttcagg
     2521 gaagcgacaa agcgaaactc aacaccctga tagaaaaagg gacgatcgag actatgcatt
     2581 cgaaagaagc gactctcgaa gagatattca tcggggtgac tggcagggag ctgagatgat
     2641 gagactggct gccaatactt tgaaggagct cagactgggc tggcggagtt attactttct
     2701 tctcgtcgtc ggagtcgcga tcgcgtactt ccttctggtg acctttctca ttcccgaaga
     2761 tctcaccgtg agcccggatg tggtggtcct gaacgagatg gaagaaggct ctcaggtcat
     2821 ggaaaatctc ttcggaggcg atatggagag cgacaaactt ttgctggcca aggatagaga
     2881 cgatcttgag aaaacaatga accggcgttt taacagtgtc ggaatagttt tgaaaggctc
     2941 tataagagag ccgggtttcg aactggtctt tcagggtcat gagagccagg atatcaggga
     3001 gctgctgaga ctttcaatat tgcagagaac gggcactctc cgctcggaag aaggatacaa
     3061 tatcgagtat cttgaagacc gaaaacccgt cgatgaagcg atccccttca acaagtcctt
     3121 tctcccggtt ctcttgatgt ccgaggcggt aatgctcgga atgttcctgg tctttgccat
     3181 gatcttcggc gagaagtccg aggaaactat aaaagcctac agcatcaccc cggggaggat
     3241 atgggagtat ctgggagcca aggtcatcgt gctcgcgatt cttggagtgc tcttcactct
     3301 catactgact cctatggtcg tcggaatcgg tccgaggtac ggcgagttat tgctgatagt
     3361 cgttgtcgga agcacccttt caacatctat agcgcttatc tttgcgagtt tctataacaa
     3421 cctctcccag tcgctcgttc ccatgcttgg gctgagtctc gtgttcggtc tgccgatgat
     3481 atcctttttc attccaggat tctcgccctg gtacctcagg attctgccca cttacaccat
     3541 acttttcgcg ctcaaggggg ccgtcttccc gggttcaatc caagttggca tctccagcct
     3601 ttggctgata gcgcttgagg gagcgatcgc cttcacgatc gccgtgcttg tttacgggca
     3661 gcgagtccga aaaaattgaa gggagatggt ttgatgaatg tgagaagagt tctcaatata
     3721 ttcagactgg acactttgaa cagccgcagg gacaaagtga tgatctacac actgctggcg
     3781 ccaataatac tgacgatcgt attcaaattg atatcgccgg attttcagag cctgtcgatc
     3841 gatttcatcg taccggagag tgaggccgaa ctggccgttc agctggacag gtacgcaaac
     3901 gtagaaaagg tagccggcat acgggaaatg aaggatgcgg tagcccgtgg aggcgaccgg
     3961 attggagtct atgagagcgg ggggaagtat gtcctggtta tgcagggcga cgaatcgcca
     4021 caggcgcgtg aactggcgga gctggcgatt tcggccgtac tccaaggaga cacatccttg
     4081 ataaatatcg acttcagcga caacggcaga gtgataccgc cggttacgct cttcggcttc
     4141 agctttgtgg tcatactctc cttcacgctg ggaggcttta tcatagggtt caatataatc
     4201 gaagagaaag agagcggagc catgagggct ttgatggtaa cgccagtttc gaaggccgag
     4261 ttccttctgg gaaggagcat aatgggaatc tccgtgccga taatccacgc gcttctggcc
     4321 gtcttgatct tcgcaatagg cggtctggat atactcaagc tcataatggt gactgtcgtg
     4381 agttcggtca taggcatcgt gatgggtttt ttgatcggag tcatgtcttc cagtcagatg
     4441 accggtcttg cgaatatgaa gataagcgtt ctgcttctgt tgatgcccgt tatgattgct
     4501 tttgttttgc ccgaaaacag acagttcata ttctactggt ctccgactta ctggtcgttt
     4561 gcggcgttga aggatattct tacacggagc gccgaatggg gaggttttct actgcaactt
     4621 ctgtggattt ctctcacgac cggactgctt ttcctgttca tgaggggaaa gataaagagc
     4681 ggactcaaga cttatcagag ttgaagcggg agagattccc gcttcatttt catcgacaaa
     4741 acccctccgg ttgaagtaga tatttttatg gggcaaaaaa taaaatgcta tataacctcc
     4801 cctgttcaat attcgtattc tggctttctg aacgatctga gtcgataatt acccatctgg
     4861 tcagatcaat cgttctttga tcgttctctg ataaatttat tccattttcc tctatctata
     4921 ttaccgtgat ggcataccag aaatgggtgc atctttggct ataatttcaa caataggaga
     4981 ttcctcttat ttcttcttgt acttgacaga aaagataacc aaatacgagg tgagaattat
     5041 gattgaagga aaggaaattg cctctgcggg tcagctgaga tatggaatgc ttgggggcgg
     5101 tgagggctct ttcatcggcg acgtgcacag aaaggcgatc gccatggacg gtatggcgga
     5161 acttgtggcc ggttgtttct cgatagacta cgaagatacg ctcaaaaccg ctaagaagct
     5221 ccgcgtcgcc gacgacagag tttacaaaga ctacgttgaa atggccagaa aggagtcggg
     5281 cagagaagat tgcatagact tcgtcgatat cgcgacaccc aacaacacgc attatccgat
     5341 cgcaaaagag tttctaaatg cgggcataaa cgttgtctgc gagaaaccgt tgaccataga
     5401 tctggcgcag gccggggaac tcgccgcgct ggcgaagaaa aaggaattgc tcttcgccgt
     5461 cacttacacc tactccggtt atgtgatggt gaaacacgct agagagatga taaagcgtgg
     5521 cgacatagga gagatcagag tcgtcatggg cgagtacgcc caggactggc tggccacacg
     5581 cctggaagac acgggaaata tgcaggcctc ctggaggacc gatccctcgc agaccggccg
     5641 atccaactgc gttggcgata tcggaagcca catagagaac acggtttcat acatgacggg
     5701 attgaagatt aagtccctgt gttccagtct ggactctttc gttcccggcc gtaagctcga
     5761 cgacaacgcc gagatactcg tgagattcga caacggcgcc aggggtgttt actgggcgtc
     5821 gcaggttgcc gtgggccacg acaacgggct gagagttagg atcttcggaa gcaagggatc
     5881 aatagagtgg gaacaggaaa accccaacta cctcagggta gcctatatcg gcggaccggt
     5941 tcagatactt tccaggggcg gaagctatat gttgccgaac gccgcgaaaa cgtcgcgaat
     6001 cccggcgggt catcccgagg gttattacga agccttcgcc aacatctaca ggaacttcgc
     6061 aacggccctg atgaaaaaga aaaaaggtga agatatttcc gatgcggatt ttccgagtgt
     6121 tgaggatgga ctcgagggag tcaaattcat aagcagagct gtcgagagtt ccgaagccgg
     6181 cagcgtatgg gtagacatgt aggaggtatg ttcatgggaa agagaccggt tactctcttt
     6241 acgggtcagt gggccgatct gacctttgag gagatctgca agaaagccag ttcctggggc
     6301 tacgacggcc tcgaaatagc ctgctggggc gatcatatgg atctagaaag ggccgcgacc
     6361 gatcccgctt atgtggagaa caggaagagt atcctcgaga agtacgatct gaagtgctgg
     6421 gcgctcggcg cccatctgga ggggcaatgc gtgggggatc tgtacgattc gaggctcgac
     6481 ggcttcgcac cggacagttg caagggcgac cccgaaaaga taagagcctg gggcataaaa
     6541 cagatgacgt tcgcccccac agccgcaaga aatatgggct gctacaccgt caccggtttc
     6601 accggctctc cggtatggaa attctggtat tccttccccc agacaacgga aaagatggta
     6661 gaagacggtt ataaagagat attcgatctc tggacgccga tattcgacag attcgacgcc
     6721 gaaggggtga ccttctccct ggaagtccat cccacggaga tcgctttcga ttactacacg
     6781 gcgaagaaac tgctcgaggt cttcaaatac agaaagacgc tcggcttcaa cttcgatcca
     6841 agccacctga tctggcaggg tgtgaaaccc cacctcttca tacgcgattt cggcgacagg
     6901 atctaccatg tccacatgaa agacgcggcc gtcacgctgg acggcagggc cggaatcctc
     6961 ggctccatgc tgactttcgg tgataaccgg agaggctgga acttcaggtc gctcggtcac
     7021 ggccatgtcg attttgaaga gataataaga gagctgaaca acatcgggta ctcggggcct
     7081 ctctcggtag aatgggaaga cagcggtatg gagagggagt acggagcccg cgaagcggca
     7141 cagttcgtaa agaaggttga tttcgagcct tcaaacatag cctttgacga cgcactcaga
     7201 agctgaggag cagggaaatg ggagaggtct tgataaagac ttcggggatc gccaaggagt
     7261 tttccggggt cagggttcta gacgatataa acatagagat agagaagggc gagattttcg
     7321 ggataatcgg tgaaaacggc gccggaaaat ctaccctggt caaaatcttc agcgggatct
     7381 acaaccctac caagggaaag ctcttcttcg aaggccggga agtcgagatc aaggatccca
     7441 tacacgccaa agctatcgga atatctctca ttccccagga gttcaacctg ataaacgaac
     7501 tcaacgttta cgagaacatc tttctcggaa gcgaactcac cggtaagggc tttctggcca
     7561 aaaaggatat gaagaagaga accagggaac tcctcaagga actcgagact actattaacc
     7621 ccgacgaaaa gatagagagg ctcagtgtgg cgcagaaaca gatggtggag atagccaagg
     7681 ccctggccca cgattcgaag cttttgatca tggatgaacc gacgaccgtc ctcacacaga
     7741 acgagataaa aatactcttc gaccttatga ggagactcaa agggcggggc gtaaccataa
     7801 tctatatctc ccacaaattg aaagaagtta aaagcatttg cgatcgcgtg atggtcctca
     7861 gggacggcat catgataagc gtaaccggga ccgtagaact ggacgagcac gagatggcga
     7921 aaagaatggt gggaagagag ttgagccagg tcttccccga aaagaccgaa ccgtcagagg
     7981 aagttgttct ggaggtcaaa aatctctccg tgcgtggtct tctgaaggat atctctttcg
     8041 atttgaggag gggggaaatc ctgggattcg cgggtctggt gggggcagga agaacggagc
     8101 tggccgaggc gatcatcggc atacgaaaga aagaggccgg tgccatgaag gtcaaaggaa
     8161 ggaccgtaga gataaaatct cccaacgacg cggtgaaaaa caagatagcc tacctctcgg
     8221 aggatcgtca gggcagcgga atattgatct ccttcgatat aataggcaac acgactttga
     8281 tatcactgcc ggcttactcc aggatactca taaaccacag actggaaaga gaaaagaccc
     8341 agaagtacgt ggataaattc aacataaggg ccgcatctct gaaaacgagg ctcgaattct
     8401 tcagcggcgg caatcagcaa aaggtttccc tcgccaaatc gctagatccg gaaccggaga
     8461 tcttcatttt cgacgaaccc accagaggaa tagacgtcaa cgccaagaga gagatctact
     8521 ggttcatcaa cgaactcgta aaggaagggg tttcgtgtat cataatatcc tccgagctcg
     8581 aggagataat cggaatgagt aaccgcgtcg tcgttatgaa agagggcacg ctcaccggta
     8641 tcctggaggg agagcatata aacgaagaag agataatgct tcacgcaacc ggactcaagg
     8701 gggtcgcatg atatggccgt tgaaacttcc agaaaacgtt ttgaatacga gaggttcggc
     8761 ccgctgatag ctctcgccat actgttcata atctcagcga tagcgagccc ttactttcta
     8821 caggtgcaga acctgctgaa cataatgagg caggtttcct acacggggat aatcgctctc
     8881 ggcatgactt tcgtgataat ctccggaggg atcgatctct ccgtcggttc catggtcgcc
     8941 ctcgtcggag gcgtggtgat actgactctg aactgggcga tgggagtttt cggcgccgcc
     9001 gcagaggcct gggccatact cttcgcactg gtcgtcggaa tactcttcgg tgcgggactc
     9061 ggcgctctca acgggctcat gattacactg ggcaagatcg ctcccttcat cgccacactg
     9121 ggtactatgg ccatattcag atccatggcc ttgtatatag gttcggccgg agtttttaga
     9181 tcccagagca acctcttccc cgaactggga atgggaaaga ttctggaaat tcccaatccg
     9241 gtcgtggttt tcatagggct cgcgataatc ttttccgtga tactcaagca gacacgttac
     9301 ggccggtacc tgtgtgccgt cggttcaaat ccaaaagtcg cgaagtattc ggccataaag
     9361 gtcaacctca ccaggtttat ctcgtacgta acggtcggaa tcaccgtcgg cttttcggcc
     9421 gttttccttt cggcgaggtt gaactccatg agttctacaa cgggcgggtc caactacgaa
     9481 ctcgacgcga tagccgccgt cataatcggc ggaacgccca tgaccggagg aacggggtcg
     9541 atcttcggca cggtgatcgg cgcgatcacg ctgggtataa tcaacaacat gctcaacatg
     9601 ctgggcgtat cgccttattt gcagggtacg gtcaaaggta tagtcatcat cggcgctgtg
     9661 ttgatccagc gcaagaggag ccagtagagc cagagataaa agcgcggtgt ttattgctat
     9721 atgcgaatat acgaaatgaa tcactaccca aaaggaggtg ttcttgtgaa aaaagcgttg
     9781 gtacttacga ttctcgtggt tttccttctc tccgcctttg cctttgcggc agagaagatc
     9841 aaaatcggag tggcaatacc cagtgcggac catggctgga cgggtggtat cgtctggtgg
     9901 tcacagaagg caattaaaga ctggaacgca aaggatccgg atgtagagtt cttcctggtg
     9961 accgccgatt ctgccgccaa gcaggttgga gacgttgagg accttatggt caagggtatc
    10021 gatgccctgg taatactggc acacgattcc gaacctctga ccccggtggt tgaaaaggcc
    10081 tacaacagcg gaatcttcgt ggtatctgtt gacagaggtc ttaccaagcc tgtcgagaac
    10141 gtttacgttg ccggagacaa ccccggtctg ggtcgtgttt ctggagagtg gctcgcgaag
    10201 gccctcaatt acaaggggga catcgtcgtg ctcgagggaa ttccctgtgt tatcaacagc
    10261 gaaagagtcg atgctttcaa cgcggttatc gcgaagtacc cggatataaa gattctcgac
    10321 tctcagcccg cgtactggca gacggagaaa ggtctggaaa taatggagaa ttacctccag
    10381 aagtacaaga agatcgacgc cgtgtgggca caggatgacg atgtgctaaa aggtgttctc
    10441 caggcctaca aggaatccgg cagaaacgat ataaaaatct tcctcggtgg cgccggctcc
    10501 aaggaaatgg tcaaaaaggt tatggacaac gatcctctag tcaaggccga cgtgacttat
    10561 cctccttcga tgatcgcaac gggaatatcg atggctgtga tggctatgag aggcgaacaa
    10621 ttgaacggct tctaccagca acagattcct tccaagatca ttctggccgc cgaactgatc
    10681 gtaccggaaa acgcggccaa ttactacgaa ccggattcga tattctaaac tgtcttccta
    10741 ccctgagcac attcccggag atcgaagtcg atctccgggt ttttacttta gcagcgtttc
    10801 tgtgaggctt tactttcgcg aacgaggccg gaggtttttg ttactccctt ttgaaaactt
    10861 tccggctttc ggaggcgtca taagcttgct gaaagaaaag gggggtgaaa cacgtgaaga
    10921 aattgagtgt cgtagcgttc ttgttgttga tggtggctcc gctggccgcc aacggagtgg
    10981 tggtacctcc gcatccggtt gaaccgggaa ccggtggctt caagatgaat tatcacaatg
    11041 ttgatatcac tctcgagaat ggaatagcga gggtaaaaat cgaggaagag ttcgagaacg
    11101 tttccaaccg cagactggaa gctgtttatc tctttcccat accttcttcg gcagttataa
    11161 gcgatttcgt gatgaaagtg ggggaccagg tcttcaaagg cgaggtcctt ccggccgacg
    11221 aggcgagaaa gatataccag gagatcgtgg ccaagttgaa agacccggct cttctcgaat
    11281 acgtcggcag ccagttgatc cgcctgagcg tctttccctt cgagccgggt gaaaagcggc
    11341 aattccatat agagtacaca caaccgctgg agatctcttc caacgcttac accatgatct
    11401 atcccttgaa aatcgacagc tttctaacgg ctccgatagg taaggtgagc attagaatca
    11461 agacaaccga cggaatagcc gaggtctttt caccgacgca tgccctgaaa gaggtccagg
    11521 cgtccggaag agagtacatc ttccaggcgg aggattttgt gccgtcttcc gatgtcgcaa
    11581 tgatcttcac cgcttcaacc gaagagattc cctcctctgt cgccactttc tgggatgaga
    11641 aagccggaga gggttatttc ctgttgactc tcatacctag attgaaggaa gagcgcatca
    11701 ttcccaagga tgttgtcttc gtgcttgata tctccggctc gatgtacggc gaaaagatag
    11761 agcaggcaaa gagggctcta gaacaggtgt tgcagatgct gaggagtgac gaccgtttcg
    11821 cgatcgtgac cttcgacgac gggatatacg acttgacagg cgggctgctg agcgcctcgg
    11881 aaaagggcgt gtggatagag aaggttcgca ggatccaggc cgacgggatg acgaacatct
    11941 acggcgccct ccaaactagt atagatatgt tcagcagggg cgacgaggga aggttcaagg
    12001 ttcttctctt cctgacggac ggtgccccga cttccgggat aacggaaacc ggcaagatag
    12061 tcggcgatgc aactaacg
//
LOCUS       DAOOLY010000045        12028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5893_length_12028_cov_5.388123, whole genome shotgun sequence.
ACCESSION   DAOOLY010000045 DAOOLY010000000
VERSION     DAOOLY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5893_length_12028_cov_5.388123"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLD15_03750"
     CDS             complement(<1..1084)
                     /locus_tag="PLD15_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB63047.1"
                     /translation="MKPRLLFITVDALGPEILARARAPVMKRIMEEGLSVQRATSHFP
                     TLTTPMMSTILTGCLPDRHGIECNTRLDLATGRVRGRLRDLKVATIGDILVENGYRVA
                     SVQHFMLEGRPGITYTQTDGGDTTGMTRLIAGCMEEFDAVFTIFQAVDAAGHRFGPFH
                     ERTMAEVEKIDAAIGRLLEVWKGREFLLVISSDHSMSYADRASGFSIEEFFASMNLKA
                     AFLSEGDSPSKIDVAMLRYPTVPIFLLSERARDLRLEIVDRLRNERELWRVYSKEELK
                     SLGNGRYGDIACTLNKGVTTAGALVETGGFGYHGTEGEEGTVIAFRGTMLKGCRIPAA
                     RLADIVPTTLKILSIESERKFDGENIW"
     gene            complement(1081..1842)
                     /locus_tag="PLD15_03755"
     CDS             complement(1081..1842)
                     /locus_tag="PLD15_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB63048.1"
                     /translation="MKKALICLFLLLGMTSMALTLKVMTYNIRHARGIDDVVDLQRII
                     DVVLSEEPDILILNEVDQGNPRTAGAHQAGEIAEALGMNLFFGPTEETSYYGNAVLSR
                     FEIVQASSVTLPQPKWLNAAKRGAAIVTVDVDGVQVLVVGTHLGLAGIKEIETELGAI
                     YSIVLESGLPAIVAGDFNVEYFDLDNRMKEVIEDLRSVNHSLGIDLHTIPSNNPGPQI
                     DYILASREFRPVNVYTVPSPASDHLPVVALLEMGR"
     gene            complement(1844..2908)
                     /locus_tag="PLD15_03760"
     CDS             complement(1844..2908)
                     /locus_tag="PLD15_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPB63049.1"
                     /translation="MRALLFVLLLSIVSLSFAAPDNVVIVIGDGMGFNHLFLSELLCG
                     GSPATRLFRVSLGLNSPADALITDSAAAATALFSGVRTVNNAAGLDPSGSPVQSVATV
                     LKERGWKVAMITNARYYDGTPAAFYAHAFRTDDLQITEDLIACDIDLFVAGGLEKLGL
                     NPFSGKPKKNGALERLAASGYAVYGLQFEKLFEPIGEKKGSMAFVSMGDNSFENVLLP
                     NEMPLPEIVRKSLELIAGPRLFLVVEAARIDDASHINDDAAVKAELLAFQKTVGLLLD
                     SFDHSRTLFLILADHETGGLSIIMGDERAINLEIAWCSGDHTASYVPILSYGAGSESF
                     SGFLHLSDVHRILLEILGVK"
     gene            complement(2927..3763)
                     /locus_tag="PLD15_03765"
     CDS             complement(2927..3763)
                     /locus_tag="PLD15_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63050.1"
                     /translation="MASRRTSKVVLYTLAYVLLIIFAFIMLMPFVWMLLSTFKDQREL
                     FEFPPKFLPKKFSLNNYIEVFKTVPFVRYYLNSLLVTFTSVVLNLFSSSLAGFAFAKY
                     RFRGREIIFKVILGAMMIPFPVTIIPLYIMVYDLGLVDSYFALIMTGSVSIFGTFLMR
                     QFIVNIPDDLLDAARIDGSSEFGTYVRIILPNLRAPLSALAVFSFMSTWNAFLWPLLV
                     VNDDSHRTVQLGVQFFTQRYGDLIHLQITAAAMAIIPIIVLYLFLQRQFIQGITMTGL
                     KG"
     gene            complement(3765..5000)
                     /locus_tag="PLD15_03770"
     CDS             complement(3765..5000)
                     /locus_tag="PLD15_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63051.1"
                     /translation="MVSGKRKVMFFTGILLMALSLFLPFASGKESLMAWDFSLAGVSL
                     PFIMVPLALACIAAFTFGKVFLTLALSLALLLLDLACLFSKEQWKPALKSKFFLDFFK
                     VAGAGWYLLAAGALLIVVAAITLFRDRKRAPYLFLLPMLAGIGFLTFFPALFALFISF
                     RRWNILIANKPFVGLANFEKAFSDEYFWRSIWISFKYALGVIPAKILIAFFFAFLIYA
                     IPKFKGFFRVVYFLPTVTSVVAVSVIWNWIYNPYYGVANYVLSVFGAPPVNWLGDPKT
                     AIWAVAAVAVWRGVGYDIIIFLAGLNDIPRTTVEASQIDGANGWQRLRYILIPLMKPS
                     LVFVFITSTIGAIQVFSEIYMMTGGNAETKTAVYYIWEYGFSRLQMGYASTMSLILFA
                     IILTISLIQMRVTRLLKEE"
     gene            complement(5041..6279)
                     /locus_tag="PLD15_03775"
     CDS             complement(5041..6279)
                     /locus_tag="PLD15_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63052.1"
                     /translation="MKRVLSILFMVVLLVSPIIAKTTITHWMHYSPARVELILEMAEE
                     FMAANPDVEIELQAIPEGEYKTKLLAALAAGSGPDVAQIPSNAMTEFYNYGLIQPFPE
                     SVITTESITGDYIPAAVEKLVIEGEVYGVPTDVQTVVLFYNPVLFEAAGLDPNRPPQD
                     WNELMEYARKTTIWQDGKMVQSGWGIAGYHPVVETFMRQAGATFWDESGEEIVFEEAQ
                     LEALKFITDAVKVEKVYVPEFGSRWTGFKQIKQAMVFGHGAMVGSFLSTGSKDLLFKT
                     ALAPAHPTTGSRANVITNWALVIMSDTDDSEAAAKWLTYITSADAQKKWMLKTGELPS
                     LKKVLEDPELLSNELYVPIFESLKYAVPTFSRGWADPASELREIAYNEIINNNMDPKT
                     ALEKAIKEINIYLEETFSQF"
     gene            complement(6283..7422)
                     /locus_tag="PLD15_03780"
     CDS             complement(6283..7422)
                     /locus_tag="PLD15_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPB63053.1"
                     /translation="MLQLNDTERTVLACLWRQDGLSRARITALTKLSKATISRVVNQL
                     MEKRLLAEQEVEPRKTRGRPSTPLTLDGNSRSVGGVNIRSNCFDVSLGDLSGRIKDFF
                     SIGHGRTDVEEGLAALPERLIERGVELDRLVSFSIVTPGIVERATASVTNAFYTGGER
                     VFLGEKLGPLDCPVTVENDANSLIYSEILFGRVRETDILYIDKGFGSSLVIDGRVFRG
                     PHGSAGELGHLQLDPRGPLCWCGKRGCLSAYFAKESLMEMFSFHLNESHLKQPERALL
                     GVLSQEYAVSSDRKFAGVFEQLLDLLGAAIANVVDFLGVETVVLNNQNPVYNSRTFDY
                     LREYIFTTAIKRKNLKFETVTVSTERLMLVPLAVALSALLEKQEV"
     gene            complement(7424..8245)
                     /locus_tag="PLD15_03785"
     CDS             complement(7424..8245)
                     /locus_tag="PLD15_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63054.1"
                     /translation="MRKAVLIVLVMAIAVSLLAFEAKNLPVNTEGIERMPWLHGNTLY
                     FVAGSYDIYRAERLADGSWGPREPVPGEINTEANEISPCIVERDGTLVMYFARYTGTE
                     RDYDFFMAVFDPVEGLWKNPVMIPELSTETQEWDIWVSSDERVAYLTTKGKFGDVEIV
                     GKRDVWKSVKVDGKWSLPVLVREVSTDGDEWSVFVDPLGRIWVDGARSDAYGSYDIYY
                     YDPSLSLVIHVEKDVNSIYNERSVWTDGKTLIFTSADRNTGMGGYDIYITDLGEE"
     gene            complement(8259..11663)
                     /locus_tag="PLD15_03790"
     CDS             complement(8259..11663)
                     /locus_tag="PLD15_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB63055.1"
                     /translation="MNWKRFLLVVGAILIVLSGFAFEAKLLPFCTSSIERMPWLFEDV
                     LYFVGNNYDIYQIVLKEDGSWGEIKQVEGEVNTLANEISPCVVRSGDDLVMYFARYSD
                     ATDYDFFRAVLDMKSGEWIGVTPVKELNTDTQEWKIWVNEGETLAYITTKGSHGGVAP
                     VGGRDIWMSEKVEGKWTTPLNVAALNTAGDEWSVFVDPLGRIWIDGSREEVLGGHDIY
                     YYDPQSKKIVHPGEDINTMYNDRSVWTDGKTLILSSSDRKGGAGSYDLYTVDLEGEVA
                     RPAVVVEAREEITATPGDLRASMDGKYVLEGQTVTVEGVALVSTGEWHDKANYFAIAD
                     ASAGVFVYGAGFIEPFVRKGDLVRVTGTLSIVGYSTDIGSLVILPSGPEDIVVLSNDA
                     DLPAPKILFSDTSGAALASYEARPVMFFGKVSQYDNETIARGFAIDGSSDSGYDDSKG
                     EVKVKFYSYAQIDISGLENGDYASVKGVLVLDKEGNYYVRPTEKSDISELQRNRLIFL
                     STVADRTLVTEEGPQEEGSEAGNGQILESAADRIFASASDGRLFLSAYSVGGTRAGNG
                     PAIYEYIDGALVKLALPGEAEYPDVKGNKLVFACRISPDDLNSSWEIYHLDLESKKLQ
                     KLLASPSDEIEPVLSPDGERVVFSRQANGRWELVELETASGRESVIEVNAKSGDFSSD
                     GGELVFQHLDESWDLVELSKLDSPIGLSRRKLLATPFDETSPAVNDDSNLIAFVADYR
                     GKGEVFLLDTTTGEVRPLFAEITESKNPFWIDAELGVAKRSETGWEMAIVEVAEEKIG
                     PFEEVENFLHKPRLTSPAMVDLNEVRFEISLKEGFVPVKAYLQGEGRTIPVMCNGNDA
                     IVTGSAAAGLYDLRIVARSASGYHSWVEPKSVYLGDSSRTVRIVQLTDIHLGLAKKPE
                     NREGFIKLLEGAKSEEPTMIVITGDVSDAAQYYPDDYAFLRSALIEHAECPVFVIPGN
                     HDSQRAGKVVGKEVWTELFGELYFGFEWGGWRYIFLDTGDSDYGLVSGMVSEEQMDWL
                     RREVGKDRKKTVLFAHHNPFDTRWSFFEKESNRQELVEVLEGGNLMLALFGHRHSDAI
                     DYHDEILAITTRKSLEGSALSYRLVILDREGIVKISQELPKASKSM"
BASE COUNT         2646 a         3552 c         3126 g         2704 t
ORIGIN      
        1 tccagatgtt ttcaccatcg aatttccttt cgctttctat agagagaatc ttcagtgtcg
       61 tcggtacgat atcggccagc ctcgccgccg gtattctgca gcctttcaac atggtgcccc
      121 ggaaagcgat cacggttccc tcctcgccct cggttccatg atagccgaat ccccccgttt
      181 ccaccagcgc accggccgtg gtcacgcctt tgttcagagt gcaggctata tctccgtaac
      241 ggccgttgcc aaggctcttc aactcttctt tagaatagac gcgccagagt tctctctcgt
      301 ttctcagacg atcgactatc tccaggcgaa ggtccctggc tctctcgctc agaagaaaga
      361 tcggcacggt ggggtatcta agcatcgcaa cgtcgatctt cgagggtgaa tcgccctcgc
      421 tcaggaaggc cgccttcaaa ttcatggagg cgaagaactc ctcgatcgaa aaaccgcttg
      481 ccctgtcggc gtagctcatc gaatgatccg aggagataac caggagaaac tcccttccct
      541 tccagacctc cagaagcctg ccgatcgccg cgtctatctt ttcgacctcg gccatagtcc
      601 tttcgtggaa agggccgaat ctgtgaccgg ccgcatccac ggcctgaaaa atcgtgaaga
      661 cggcgtcgaa ctcctccatg caaccggcga tgagacgcgt catccccgtc gtatcgcccc
      721 cgtcggtctg ggtgtaggta attcccggac gtccttcgag catgaaatgc tgtaccgagg
      781 ccaccctgta accgttctcg accagtatat cgcctatcgt cgccactttc agatcgcgca
      841 accttcccct caccctgcct gtcgccagat ccagtctggt attacactcg attccatgac
      901 ggtcggggag gcagccggtg agtatggtgc tcatcatagg cgtcgtaagc gtggggaagt
      961 gggaagtcgc cctctgaaca gacaaccctt cctccattat ccgcttcatc accggcgccc
     1021 ttgcccgcgc gaggatttcc ggaccgagcg cgtcgacggt tatgaaaagc aaccgcggtt
     1081 tcatcttccc atctccagta aagccacaac cggcaggtga tcggaggccg gcgaggggac
     1141 ggtgtagacg ttgaccggcc ggaattcacg gcttgccagg atatagtcga tctgcgggcc
     1201 ggggttgttc gaaggtatcg tatgcaggtc gatacccagc gaatggttca ccgatctcag
     1261 gtcctctatc acttccttca tgcggttatc gagatcgaaa tactctacgt tgaagtctcc
     1321 ggccactatc gccggcaacc cgctttccag cactatggag tagatcgcgc ccagctcggt
     1381 ctcgatctcc tttataccgg ccagaccgag atgggtgcct accaccagaa cctgcactcc
     1441 atctacatcg acagtcacga tggccgcccc gcgctttgcg gcatttagcc acttcggctg
     1501 gggcagggtg acgctactgg cttggactat ctcgaacctg gagagtacgg cgttgccgta
     1561 atagctcgtc tcttcggttg gcccgaagaa gaggttcatg ccgagcgctt cggcgatctc
     1621 cccggcctgg tgcgctccgg ccgttctcgg attgccctga tcgacttcgt tgagtataag
     1681 gatgtctggc tcttcggaaa ggacgacgtc tattatcctc tgcaggtcga cgacatcgtc
     1741 gatccccctg gcgtggcgaa tgttgtaggt catcaccttc agcgtcaggg ccattgatgt
     1801 cattccaagc aaaaggaaga gacagatcag agcttttttc atctcacttc acccccagaa
     1861 tctccagaag tattctgtga acatcgctca gatggagaaa gccggagaag ctctcgctcc
     1921 cggctccgta agagaggatc ggcacgtaag aggccgtgtg gtctccgctg caccaggcga
     1981 tttcgaggtt gatcgccctc tcgtcgccca tgattatact caggccgccg gtctcgtgat
     2041 cggccagtat caggaagaga gtcctcgagt ggtcgaagct gtccagaagc aacccgacgg
     2101 tcttctggaa ggcaagcagc tcggccttga ccgccgcatc gtcgtttatg tgcgaagcgt
     2161 cgtctatcct ggccgcctcg accaccagaa agagtctcgg cccggcgatc aattcgaggc
     2221 tcttgcggac gatctcaggc agaggcatct cgttgggcag taaaacgttt tcgaagctgt
     2281 tgtcgcccat cgagacgaag gccatgctcc ccttcttctc gccgataggt tcgaaaagct
     2341 tttcgaactg caggccgtaa acggcgtaac cactggctgc cagtctctcg agcgctccgt
     2401 tcttttttgg ctttccgctg aaggggttca ggccgagttt ttccagtccg ccggctacga
     2461 agagatctat atcgcaggct attagatcct cggttatctg gagatcgtcg gttctgaagg
     2521 cgtgcgcgta gaaagcggca ggcgtgccgt cgtaatatct ggcgttggtt atcatcgcga
     2581 ccttccagcc cctctctttc agcaccgtcg ccaccgactg cacggggctg cccgatggat
     2641 ccagcccggc ggcgttgttg accgttctga ctccggagaa cagcgcggtg gccgcggcgg
     2701 ccgaatcggt tatcagggca tctgccggtg aattgagacc tagagagacc ctgaagagtc
     2761 tcgtcgccgg actccctccg cagagtagtt ccgagagaaa gagatggttg aagcccatcc
     2821 cgtcgccgat gacaataacc acgttgtccg gcgcggcgaa agacagcgag acgatcgaca
     2881 gcagtaaaac gaacaacaat gctctcatcc aaaatcctct cctttctcaa cctttgagac
     2941 ccgtcatggt tattccctgg atgaactgcc tctgcaggaa gaggtacagc acgattatcg
     3001 gaatgatggc catggcggcc gccgttatct gcaagtgtat cagatctccg tacctctgag
     3061 tgaagaactg gacgcccagc tgcacggtcc tgtggctgtc gtcgttgacc accagcaaag
     3121 gccagagaaa ggcgttccag gtcgacatga aagaaaacac ggccaacgcc gagagcggcg
     3181 ccctcaggtt cggcagaatg atccgcacat acgtgccgaa ctccgaagat ccatctattc
     3241 gcgcggcgtc gagcagatcg tcgggtatat tgacgatgaa ctgcctcatc agaaaagtcc
     3301 cgaagatgct caccgaaccg gtcattatca gcgcgaagta ggaatcgaca agtccgaggt
     3361 cgtaaaccat tatgtacagc ggaataatgg tgaccgggaa gggtatcatc atcgccccca
     3421 gaatgacctt gaagataatc tcccgacccc tgaagcggta cttggcgaag gcgaagccgg
     3481 ccagacttga agagaagaga ttcagaacga ccgaggtgaa ggtcaccagg agactgttga
     3541 gatagtacct cacgaagggg actgtcttga atacttcgat gtaattgttc agcgagaact
     3601 ttttcggcag gaacttcggc gggaactcga agagctccct ctgatccttg aaagtagaga
     3661 gaagcatcca gacgaagggc atcagcatta tgaaggcgaa gatgatcaaa agaacgtacg
     3721 ccagcgtgta cagaacgacc tttgaagttc ttctggaagc catctcactc ctcctttaaa
     3781 agccgtgtta ctctcatctg tatcagggag atcgtcagta tgatcgcgaa gaggatcagc
     3841 gacatcgtac tggcgtaacc catttgaagc ctgctgaacc cgtattccca gatgtagtag
     3901 acggccgttt tcgtttcggc gttgccgccg gtcatcatat aaatctccga gaaaacctgt
     3961 atcgcaccga tcgtggaggt tatgaacacg aagacgagcg acggcttcat cagagggatc
     4021 agtatatacc tcaacctctg ccagccgttc gcgccgtcta tctgcgaggc ttccaccgtg
     4081 gttctgggta tatcgttgag acctgccaga aagatgatga tatcgtaccc caccccacgc
     4141 cagacggcga cggccgcgac ggcccagatg gccgttttgg gatcgccgag ccagttgacc
     4201 ggcggtgcgc cgaacacgct cagcacatag ttcgccacgc cgtagtaggg gttgtatatc
     4261 cagttccaga tgacgctcac cgccacgacg gaagtgacgg tcggcaggaa gtaaacgacc
     4321 ctgaaaaagc ccttgaactt cgggatggcg tatatcagaa aggcgaagaa gaaagctatc
     4381 agtatctttg ctggaatcac gcccagagcg tacttgaaac ttatccagat cgaccgccag
     4441 aagtactcgt cagagaaggc cttttcgaag ttggccagac ccacgaaggg cttgttcgcg
     4501 atcagtatgt tccaccttct gaaacttata aaaagcgcga agagggccgg aaagaacgtc
     4561 aaaaaaccta ttcccgccag catgggcaga agaaaaaggt acggtgccct cttacggtct
     4621 ctgaaaagcg ttatcgcagc cacgactatc agcagagcgc cggcggcgag gagataccag
     4681 cccgccccgg ccactttgaa gaaatccaga aagaacttgg acttcagggc cggtttccac
     4741 tgctccttag aaaagaggca ggcgagatcg agaagcaaca gggccagcga aagagcgagc
     4801 gtcagaaaaa cctttccgaa cgtgaaggcc gcaatacagg ccagagcgag cggaaccatg
     4861 atgaacggca gactgacgcc ggccagagag aagtcccagg ccatcagact ttctttaccg
     4921 ctcgcgaagg gcaggaaaag cgataacgcc atcagcagta ttcccgtgaa aaacatcact
     4981 ttgcgtttac ccgacaccat accttctacc tccaatcaag ggccttttca ggcccttgag
     5041 tcagaactga ctgaaagtct cctcgagata aatgttgatc tcttttatcg ccttctccag
     5101 tgcggttttc ggatccatgt tgttgtttat gatctcgttg taggctatct cccgcagctc
     5161 cgaagccgga tcggcccagc cccttgagaa ggtcggcacg gcgtatttta gagactcgaa
     5221 aattggcaca taaagttcgt tggagagcag ctcgggatct tcgagaacct tcttcagcga
     5281 agggagttca ccggtcttca gcatccactt tttctgcgca tcggccgagg ttatgtaggt
     5341 cagccacttc gcggcggcct cgctgtcgtc ggtatcgctc attataacaa gcgcccagtt
     5401 ggttataacg ttggctctcg aaccggtcgt cgggtgggcc ggagcaagcg ccgtcttgaa
     5461 gagcagatcc ttgcttcccg tgctcaggaa cgaaccgacc atggcgccgt gaccgaagac
     5521 catggcctgc ttgatctgct tgaaaccggt ccatctgctt ccgaactcgg gaacgtagac
     5581 cttctcgacc ttgaccgcgt cggtgatgaa cttcaaagct tcgagctggg cctcttcgaa
     5641 gactatctcc tcgccgcttt catcccagaa agtggctccg gcctgacgca tgaaggtctc
     5701 cacgacgggg tgatagccgg cgattcccca gccggactgc accatcttgc catcctgcca
     5761 gatcgtggtc tttcgcgcgt attccatgag ctcgttccag tcctgcggcg gtctgttcgg
     5821 gtcgagcccg gccgcttcga agagaacggg attgtagaag agaaccacgg tctggacatc
     5881 ggtgggaacg ccgtaaacct ctccctctat taccagcttc tcgactgcgg ccggtatgta
     5941 gtcaccggtg atgctctcag tggtgatgac gctttcggga aagggctgaa tcaaaccgta
     6001 gttgtagaac tcggtcatgg cgttcgaagg gatctgggcg acatccggcc cgctcccggc
     6061 cgccagggct gcgagcaact tggtcttgta ctctccctcg ggaatggcct gcagctcgat
     6121 ctcgacgtcg gggttggccg ccatgaattc ctcggccatc tcgagtatca gctcgacgcg
     6181 ggccggggag taatgcatcc agtgagtgat cgtcgtcttc gcgatgatcg gagagacgag
     6241 aagcaccacc atgaaaagta tcgacagaac tctcttcata attcacacct cctgtttttc
     6301 gagcagagcg gaaagcgcaa ctgccagcgg caccagcatc agcctttccg tagataccgt
     6361 tactgtttcg aacttcagat tctttctctt gatcgccgtg gtgaagatgt actcccgaag
     6421 ataatcgaaa gtccttgagt tgtacactgg gttctggttg ttaagcacga ctgtctccac
     6481 accgagaaaa tcgaccacgt tggctatcgc cgcgccgaga aggtccagca actgctcgaa
     6541 gaccccggcg aactttctgt ccgacgagac ggcgtactcc tggctcagaa ctcccagcag
     6601 cgccctctcg ggttgtttca gatgagactc gttcaggtga aaggagaaca tttccataag
     6661 actctctttg gcgaagtagg ccgagagaca gcctctcttc ccgcaccagc agagcggtcc
     6721 gcggggatcg agctggagat gcccgagctc cccggcactc ccgtgaggcc ctctgaaaac
     6781 cctgccgtcg atcaccagcg aagagccgaa gcctttgtct atatagagga tgtcggtctc
     6841 ccggactctg ccgaagagga tctccgagta tattagggaa ttggcgtcgt tttcgaccgt
     6901 caccggacag tcgagagggc cgagtttctc tccgagaaag actctttccc cgcctgtgta
     6961 gaaggcgttc gtgaccgagg ccgtcgccct ttcaacaatt cccggagtga cgatgctgaa
     7021 agagacgagc ctgtccagct ctactcccct ctctataagc ctctcgggca aagccgccag
     7081 cccctcttcc acatcggttc ttccatgtcc gatactgaaa aaatctttga tccttccgga
     7141 aagatcgccc agcgaaacat cgaagcagtt ggagcgaatg ttcaccccgc cgaccgatct
     7201 tgaatttccg tcgagagtga gcggcgtcga aggacgtccc cgggtctttc tcggctcgac
     7261 ttcctgctcg gcgagcaatc gcttttccat cagctggttc acgaccctcg agattgtcgc
     7321 cttgctcagt tttgtgagcg ccgttattct ggcgcgagaa aggccgtcct ggcgccacag
     7381 gcaggcaagg accgtcctct cggtatcgtt caattgaagc aaatcactct tctcccagat
     7441 cggttatgta gatgtcgtag ccgcccattc cggtatttct atcggccgat gtgaagatca
     7501 gagtcttgcc gtctgtccag acgcttcttt cgttgtagat agagttgacg tccttctcga
     7561 cgtggatcac cagagagagc gaaggatcgt aataataaat gtcgtagctg ccgtaagcgt
     7621 cgcttctggc gccgtcgacc cagatcctgc caagcggatc gacaaagacc gaccactcgt
     7681 ctccgtcggt cgagacctct ctgaccagaa ccggcagact ccactttcca tcgaccttca
     7741 cggatttcca gacgtctctc ttacccacta tttcgacatc gccgaacttt cccttggtgg
     7801 taagataagc caccctctca tcgctactga cccatatgtc ccattcctgg gtttcggtgc
     7861 tcagctcggg tatcatcacc ggattcttcc agaggccttc gaccggatcg aaaaccgcca
     7921 tgaaaaagtc gtaatccctt tcggtgcctg tgtaccgggc gaagtacatg accagagttc
     7981 catccctctc gacgatgcag gggcttattt cgttagcctc tgtgttgatt tcccctggca
     8041 ccggttccct cggtccccag gagccgtcgg ccagtctctc ggcacggtaa atgtcgtagc
     8101 tgccagccac gaagtacagc gtattcccgt gaagccatgg cattcgctct atgccctcgg
     8161 tgttcacggg cagatttttg gcctcgaaag ccagaaggga gacggctatc gccataacca
     8221 gaacgatcag tacagcctta cgcataaaat cacctctctc acatcgattt tgaagctttc
     8281 ggtaactctt gcgagatctt cacgattccc tctctatcga gaatcactaa cctgtagcta
     8341 agcgcggaac cttcgagact ctttcttgtg gtgatggcaa gtatttcatc gtggtaatct
     8401 atcgcgtcgg aatgcctgtg gccgaagagg gcaagcatca agtttccacc ctccagaact
     8461 tcgaccagct cctgccggtt cgactccttt tcgaagaaac tccacctcgt gtcgaagggg
     8521 ttgtggtggg cgaagagaac cgtcttcttc cggtctttgc cgacctctcg cctcaaccag
     8581 tccatctgtt cctccgacac catgccgctg accagaccgt aatcggagtc tcccgtgtcc
     8641 agaaagatat agcgccatcc gccccactcg aagccgaagt agagctcgcc gaagagctcc
     8701 gtccacactt cctttccgac gacctttccg gccctctggc tgtcgtggtt tccgggtatc
     8761 acaaagaccg gacattcggc gtgctcgata agcgcgctcc tgaggaaggc gtaatcgtcc
     8821 ggatagtact gggctgcatc ggaaacgtct ccggtaatga ctatcatcgt cggctcttcg
     8881 ctctttgcac cctcgagcaa ctttatgaac ccttcgcggt tctccggctt tttcgccagg
     8941 cccagatgaa tgtcggtgag ctggacgatc cgaaccgtcc gggagctatc gccgagatag
     9001 acgctcttgg gctctaccca ggagtggtaa ccggaggcgc tccgcgctac gatcctcaga
     9061 tcgtaaagac cggcggcagc gctgcctgta actatcgcgt cgttaccgtt gcacatcacc
     9121 ggtatcgtcc ggccttcccc ttgaaggtag gccttcaccg gcacgaaacc ctctttcaga
     9181 cttatctcga atcgaacttc atttagatcc accatggccg gcgatgtcag gcgcggcttg
     9241 tggagaaaat tctcgacttc ttcaaaaggc ccgatttttt cttccgcaac ttcgacgatc
     9301 gccatttccc agccggtttc cgaccgcttc gcaacgccga gttcagcgtc tatccagaag
     9361 ggattcttcg actctgtaat ttccgcgaag aggggtctca cctctccggt ggtcgtatcc
     9421 agcaggaaga cttcaccctt accgcgatag tcggctacga aggctatcaa gttagagtcg
     9481 tcattaactg ccggagatgt ttcgtcaaag ggagtggcca gaagttttct ccgggaaagt
     9541 ccgatagggc tgtccagttt cgagagttca acgagatccc agctttcgtc gagatgctgg
     9601 aaaaccagtt ccccgccgtc ggacgaaaag tcgcccgact tggcgttgac ttcgatcaca
     9661 ctctccctgc cagaggccgt ctcaagctcg accagttccc agcggccgtt ggcctgtctg
     9721 gaaaagacca ccctttcccc gtccggagag agtacgggct cgatctcgtc gctgggagaa
     9781 gcgagaagtt tttgcagctt cttcgattca aggtcgagat ggtagatctc ccaactggag
     9841 ttcagatcgt ctggcgagat tctgcaggcg aaaacgagtt tattcccttt cacgtcgggg
     9901 tactcggcct cgcccggcag tgcgagcttt accagcgctc cgtcgatgta ttcgtagatc
     9961 gccgggccgt tgccggctcg agtccccccg acggagtacg ccgaaagaaa caaccttccg
    10021 tccgacgcgc tggcgaagat cctgtcggcc gcgctctcaa ggatctggcc attgcctgcc
    10081 tcgctcccct cctcctgcgg accttcctct gtaactagag ttctgtcggc aaccgtagag
    10141 agaaagatca gccggttccg ctggagctcg cttatgtcgc ttttttcggt cggcctcacg
    10201 tagtagttgc cttccttgtc gagtaccagg acgcccttca cggaggcgta atcgccgttc
    10261 tccagccccg agatatctat ctgcgcgtag gaatagaatt tgaccttgac ctcgccttta
    10321 gaatcgtcgt acccggaatc gctcgagccg tctatcgcga agcccctggc gatggtctcg
    10381 ttatcgtact ggctcacttt cccgaagaac atcaccggtc tggcctcata agacgccagg
    10441 gctgccccgg aagtgtcgct gaagaggatc ttcggcgccg gcagatcggc atcgttagag
    10501 aggaccacga tatcctccgg accggaggga agaatcacca gactgccgat atcggtggaa
    10561 taacccacga tcgagagcgt gcccgtcacc ctaaccaggt ctcccttcct gacgaaaggc
    10621 tctatgaaac cggccccgta aacgaagacc cctgccgaag cgtcggctat cgcgaagtaa
    10681 ttcgccttgt cgtgccactc tcccgtcgag accagcgcaa cgccttcgac ggtcactgtc
    10741 tggccttcga gaacgtactt gccgtccatc gacgcgcgaa gatcgccagg tgttgcagtt
    10801 atctcctccc tggcctccac gaccacggcc ggccgggcca cttcgccctc cagatcgaca
    10861 gtgtacagat cgtaacttcc ggcgccgccc ttcctgtcgc tcgaagagag aatcagcgtc
    10921 tttccgtcgg tccagacgct cctgtcgttg tacatggtgt ttatatcttc acctggatgg
    10981 acgatcttct tcgattgcgg atcgtagtag tatatatcgt ggccgccgag aacctcttcc
    11041 ctcgatccgt cgatccagat tctgcccaac ggatcgacga agaccgacca ctcgtcgcca
    11101 gccgtgttga gggcggccac attgagaggg gtcgtccact tgccctcgac cttctcggac
    11161 atccatatat ctcttccacc gaccggagcg acgccgccgt gcgagccctt cgtcgttata
    11221 taggccagag tctcgccctc gttgacccat atcttccatt cctgtgtgtc ggtgttcaat
    11281 tccttcaccg gcgtcacacc gatccattca ccgctcttca tatccagaac ggccctgaag
    11341 aaatcgtagt cggtggcgtc gctgtaacgg gcaaaataca tgaccagatc atcgccgctc
    11401 cgtaccacac acggactgat ctcgttggcc agagtgttga cctcgccctc cacctgttta
    11461 atttcgcccc agctgccgtc ctctttcaga acgatctgat aaatatcgta gttgtttccg
    11521 acgaaataga gaacatcttc gaagagccac ggcatcctct ctatgctgct cgtacagaag
    11581 ggtaatagtt tcgcttcgaa agcaaatccc gacagaacga tcaggatcgc tcctacaacg
    11641 agtaaaaatc tcttccaatt catatagaaa cctccatacc acaccaaaaa tatgagccga
    11701 caggggtagc aattagactt tacaactaac ctactggacg aaccaaaccg ttatagccgt
    11761 tgaatttcga tcagactaaa ctacaggtca tctcgcgcgg aaataacacc ccttttctca
    11821 acgggagccg tccggtattt agtttcacct cgagactaat aaaccaccga aagggcgaga
    11881 agaggagaga aagagagagt gagagaaaag agagagcgaa aatggggact gaccggttcc
    11941 tgtggtctgt acccgatttt cgcattacca acaaccaaca acgaacaacc aacaacctct
    12001 cctatcatcc cactatggtt ctcgctca
//
LOCUS       DAOOLY010000046        11981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5920_length_11981_cov_5.388982, whole genome shotgun sequence.
ACCESSION   DAOOLY010000046 DAOOLY010000000
VERSION     DAOOLY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5920_length_11981_cov_5.388982"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="purH"
                     /locus_tag="PLD15_03795"
     CDS             <1..721
                     /gene="purH"
                     /locus_tag="PLD15_03795"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPB63056.1"
                     /translation="ALKHTNPCGVGLADTTLEAWKKAYGCDRVSVFGGIVATNGTVDG
                     PLAGELKNIFLEIIMAPAFTSEALEMLSARKNIRILEIDPVFKSNGYDFKSIPGGMVV
                     QDRDNALLGEGLRTVTAKKPDEREMEDMLFAWKVVKHVKSNAIVLAREGQTLGIGPGQ
                     TSRIWALENAIRQSNYDLSGAVLASDGFFPFPDCVEAAAKAGIGAIIQPGGSIKDEDS
                     IEAADRLGLSMVFTSMRHFKH"
     gene            733..1992
                     /gene="purD"
                     /locus_tag="PLD15_03800"
     CDS             733..1992
                     /gene="purD"
                     /locus_tag="PLD15_03800"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPB63057.1"
                     /translation="MRILVIGNGGREAALAWKLFQSEGLERLFCAPGNPGTSSIAENV
                     PLDPCDIDGLLTFAKEQEIDLAIVGPEKPLSMGMVDRFRERDLAVFGPTRDCARLETS
                     KIFAKGFMNRHGIPTARHASARYYLQALAELESFELPLVIKADGLAAGKGVFIADRQE
                     EAHRILKDLLIDDSLKEAGSSVVIEEYLSGRELSVFVLSDGKRMVELAEARDFKKAFD
                     GDLGPNTGGMGAISPVPDYTDRLREEFRDKILLPTVEGLAREGLTYTGLLYFGLINTP
                     VGLKVLEFNCRFGDPETQAILPRIDFDLARVLYDCARGQLKEERLRLKQECAVTVVAC
                     SKGYPGRYVTGCEVSGLTEGNCTLFQAGTGYSGEKVVTTGGRVLAVTALERDFGEAVR
                     KAYGALEKIRFKGMTFRKDIGVEFDVN"
     gene            2018..3316
                     /gene="purB"
                     /locus_tag="PLD15_03805"
     CDS             2018..3316
                     /gene="purB"
                     /locus_tag="PLD15_03805"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPB63058.1"
                     /translation="MIERYALSPLKELWELKAQYERWLEVELAVVKAYEETGLAPAGM
                     AEKIRSKARIDVEGIAEIEKVVDHDVIAFIKYVTGDMGDEARYFHLGLTSSDIVDTAL
                     SLALRKSGLAIIQALTELSQALAERALIYKDTVCMGRTHGIHAEPTSFGLKFLSFMVE
                     TDRNIERLRRSVEASSVGKLSGAVGNYANIPPEVEAIALREIGLRPTEVSTQIVPRDI
                     HAEFISAMAIAASSIERMAVEFRHLQRTEVLEVQEPFKKGQRGSSAMPHKKNPIICER
                     LTGMARLLRGYAGAALEDIALWHERDISHSSVERIMLPDATMLLYYMAKKSIDLAKDL
                     VVYPERMLENFKASRNLVFSQRVMLTLVEKGMAREEAYKLVQELSLNCWNEGRDFKEA
                     VAKSREIEKYIDSREIDELFDPEYYLRNVDPIFKRILDGG"
     assembly_gap    3446..3545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3546..4729
                     /locus_tag="PLD15_03810"
     CDS             <3546..4729
                     /locus_tag="PLD15_03810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPB63059.1"
                     /translation="KDFEWIVRFSGGANAGHTIYVDGKKYVNHLLPSIQPRSASRGFL
                     GAGMVIDVEQLINEFETLEADFPDISCRFYIDPEAFAVLPWHKEEDVLLETMRKEPIG
                     TTGRGIGPSYTDKVSREGLKLYTLFDENLLKERLESIYHMKQNIYNNRLTVSPEDMFK
                     YLQRLRGELERLKVNFTSAVDMSRVFRSTSVLFEGAQGVLLDLDFGTYPFVTSGACMA
                     HGVSSVGFSTFELDSVYGVLKAYTTRVGAGPFPTEDFTETGEKIREMGKEYGATTGRP
                     RRVGWLDLPALRYARIRSGLTGFVITKGDVLNGLEEVKVCVAYDVDGVVKEVPSTSYD
                     FFRAKPIYETVKGWPTTDHINFLKYMTFIERETGVEIDYISYGPKTEEMKTRNDLIIN
                     I"
     gene            4762..6150
                     /gene="cysS"
                     /locus_tag="PLD15_03815"
     CDS             4762..6150
                     /gene="cysS"
                     /locus_tag="PLD15_03815"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB63060.1"
                     /translation="MDIRLNNTMSREKEVFKPLTAGEVKLYTCGLTVYNYAHIGNLRA
                     YIFADTLKRMFLFNDYKVNHVMNITDVGHLTGDEDEGEDKMEAGARREGKTVWEIVDF
                     YTKAFFKDLERLRILFPTTTCRATEHVGDMIEMIKKIEANGYTYIAGGNVYFDTSKLP
                     DYGKLARLQLDEDRMRSRVESDPYKRNPFDFVLWFTRYKYTDHAMQWDSPWGRGFPGW
                     HIECSAMSSKYLGESFDIHTGGIDHIPIHHTNEIAQSEAAFGHEWVNYWLHSEFLVIG
                     EGEKMSKSLGNFITLQTLIDMGYDPMDYRYYLLGAHYRKQLAFTFEALDGARNAMKRL
                     KIGIAELKESQSPSAFADPQLLEEFHEAINDDLNTPRALAVLWKVIDSEDLSAGEKLA
                     LIEQFDRVLALGLAEVESDTIPGEIEELALQRKQARKDRNWKRADELRDLISEKGYEI
                     LDDRDGYKIRKK"
     gene            complement(6198..7202)
                     /locus_tag="PLD15_03820"
     CDS             complement(6198..7202)
                     /locus_tag="PLD15_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63061.1"
                     /translation="MDIRFATKEDNEKLLDIERRSAQEGKIWVVGYKENFFDRLKYYG
                     NGSILLAEEKGDIIGCIGIAFDEYLVDSVPRKAIYLFGLRTNPAYRMKIARWLKAIIQ
                     ELENRYGESEFDFAYASVKADNTASKKILAHMGFSRFATLDFYACPVLHRIKSTGVYV
                     ERPDMNRVRGLYSGMSGRFDLVPKHTRIFEPMIEEERLKLFTGEGASALVFDTSGESD
                     FGIAKLSPGLRAFQVAGRSLLSPLARVPGMKERLRIWDVLILDYGTPRAARRVVAEIH
                     KEAWKEKVTLLNFARDSTLGSLKSAIGPLSFKIPFDIMIFEKKEIPRKGRPVIWVPAL
                     "
     gene            complement(7218..9167)
                     /locus_tag="PLD15_03825"
     CDS             complement(7218..9167)
                     /locus_tag="PLD15_03825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPB63062.1"
                     /translation="MKKIRLEDVHRYSAVGDVHFLPNGREFLFVHKTIDRENNKYLSN
                     IWSGDLSTGKVTQLTRGDKDGSPVVDPEGKSLLFTSRREKDAKGTELYLLQLGGGESR
                     LVKTLKGGFSSISWVDEGNILFTTKVAPGENPEKPAEEEPVEKRIYVIDKIPFVSNGS
                     GFTENRISQLHTLNVETGKMKPLEAVKGDVQSVELSPDRKRAAIIVKEDAERRPRWNN
                     LYILDLKGGSTERIGDDSISVYACVWASSDELFVLGTDLAKGFPTNPYFNFVDLESKE
                     LKPLRRDLDLYFGNSLNSDVRGLSGESLRVKKGKLYSIITSGPDSTIVSLDKSGEVED
                     VMLSDGSIDCFDINDSGDLIYTKMTPVQPLELYCSKATGTKKLTNFNSWMKGYQLSAP
                     ESFKIQASDGVVIDGWIMKPVDFDEKGRYPAIVEIHGGPKTAYGNGYIHEFQALAASG
                     YAVVYCNPRGSSGYGTDFADIRGHYGERDFDDIMEVLEFVVNEYDFIDEERLGVTGGS
                     YGGFMTNWIVGHTDVFKAAVSQRSISSWISFFGTTDIGYYFAPDQVGDDFFQNLEGYL
                     RQSPLTYAPNVETPILFIHSLEDYRCWVPEAMQLFTTLRYLGKEARMVLFPGENHELS
                     RGGMPVHREKRLKAIVEWFDTHLKK"
     gene            9279..9881
                     /locus_tag="PLD15_03830"
     CDS             9279..9881
                     /locus_tag="PLD15_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HPB63063.1"
                     /translation="MVILSHFDPFGGDGINASKIVAQKVAGMRSDLRVVELPTVFDDA
                     FIPLMTEIERTAPEAIIMLGQAAGRTAVTPEKVAINWKESTKADNNGYIARGERISIS
                     GADAYFSTLPLVEIIDSLQRDGLPLSLSFTAGTFVCNYLFYRTMEYLVSTGKDFPVGF
                     VHIPCIPQQTVGKNGLASMDAALSAAILSRMIDVIVGDGR"
     gene            9878..10627
                     /locus_tag="PLD15_03835"
     CDS             9878..10627
                     /locus_tag="PLD15_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPB63064.1"
                     /translation="MIIGGAALDIAWEDPAKNMEKIADIFDQAFKKHVELLVFPEMSL
                     SGFSLNPEKTCTPYSDSFFDGLVRKTSIAAVYGHVEREESGYVNCASFLSTDLGPVTY
                     GKRKLFTYADEHESYVPGRQAISLIHQGIEISLNVCYDLRFPELFRENVPAELMVVIA
                     NWPSARARHWEVLLRARAVENQCFTLGVNRTGSDGKGVEYGGTKSMLIDHNGDEVEVE
                     KVGELLIWELKDEAVERMRAWRRKFPALEDM"
     gene            complement(10635..10895)
                     /locus_tag="PLD15_03840"
     CDS             complement(10635..10895)
                     /locus_tag="PLD15_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPB63065.1"
                     /translation="MLIRICMGSACYVLGASKIAKRLQELIDEKALADSIDFKASFCM
                     GPCSEGVVVEVDGQRHCRLSPENVEEFFQREILNQIEKRSDR"
     gene            11049..11726
                     /locus_tag="PLD15_03845"
     CDS             11049..11726
                     /locus_tag="PLD15_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB63066.1"
                     /translation="MKILVVEDEHDLGLLLKEVLESNNYAAELSAEGEEGLYMALNGP
                     FDLVVLDVLLPGLSGWEVLEGMRRKGCTTPVLMLTALDSVDNKIRGFNLGADDYLSKP
                     FDMRELLARIQSLLRRTAPVAAPTNELVCGDLVLNISDKSVYRGSTKIELRKKEYQIL
                     EYLMINKRRVISKSELEDHIWNEEDELWSDVIRSHIKNLRKKIDGGYRKKLIKTVRGM
                     GYEISEE"
     gene            11707..>11981
                     /locus_tag="PLD15_03850"
     CDS             11707..>11981
                     /locus_tag="PLD15_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPB63067.1"
                     /translation="MKSVKSEELSFKSARIRWTTFFTIVVVSIVSILCVIVFFFALRY
                     ETRSSYAGLNQMADRIATRIGRAPLITLERILRNYAENPDLFTAENE"
BASE COUNT         2930 a         3043 c         3275 g         2633 t
ORIGIN      
        1 agccctgaaa cataccaatc cctgcggcgt cggcctggcc gacacaactt tagaggcgtg
       61 gaaaaaggct tacggttgcg atcgggtatc tgtgttcggg ggcatagtgg cgaccaacgg
      121 gaccgtcgat ggcccgctgg caggagagct gaaaaacatc tttcttgaga taataatggc
      181 gccggccttc acatccgaag cgcttgagat gctgtcggcc aggaagaaca taaggatcct
      241 ggagatcgat ccggttttca agagcaacgg gtacgatttc aaatcgatcc ccggtggaat
      301 ggtggttcag gacagagata acgccctgct gggggaggga ttgagaaccg ttacggcaaa
      361 aaaacccgac gagagggaga tggaagatat gctcttcgca tggaaggtcg taaaacacgt
      421 caaatctaac gccatagttc tggccaggga aggtcagacc cttggaatag gaccgggtca
      481 gaccagcagg atctgggccc tggagaacgc gataagacaa tcgaattacg acctgagtgg
      541 ggccgttctg gcgtcggatg gtttctttcc cttcccggac tgtgtggaag cggccgcgaa
      601 ggccgggatc ggagctataa tccagcccgg gggttcgatc aaagatgaag actccataga
      661 ggcggccgac agactcgggc tttcgatggt ctttacgagc atgcgtcatt tcaagcattg
      721 agggcgaaaa tcatgagaat actggtaata ggaaacggcg gccgtgaggc cgcacttgca
      781 tggaaactct tccaaagcga agggctggag aggctcttct gcgcgccggg aaaccccgga
      841 acatcctcaa tcgcggaaaa cgttccgtta gatccctgcg atatagacgg tctgctgacc
      901 tttgcaaaag aacaggagat agacctggct atcgttggtc cggagaaacc tctttcaatg
      961 ggcatggtcg atcgcttcag ggaaagggat ctggccgtat tcggtcccac cagagattgt
     1021 gccaggctcg aaaccagcaa gatcttcgcc aaaggattta tgaaccgcca cggtattccc
     1081 acagcccgtc acgcgagcgc caggtactac ctgcaggctc ttgccgagct ggagagtttt
     1141 gagctacccc tggtgataaa ggccgacgga ctggccgcgg gtaagggtgt attcatagca
     1201 gaccggcagg aggaagcgca tcgaatactc aaggatttgc ttatcgacga ctctctgaaa
     1261 gaggccggct caagtgttgt tatagaggaa tacctgagcg ggagagagct ttccgtcttc
     1321 gtcctgtccg acggaaagcg tatggttgag ctggcagagg cccgggattt caagaaagcc
     1381 ttcgacggag acctcggccc caacaccggt ggaatgggag ccatctctcc cgtacccgat
     1441 tatacggacc ggcttcgcga ggagttcagg gataaaatcc ttctcccgac ggtggaaggg
     1501 ctagcgagag aaggtctcac ctacacggga cttctatatt tcggcctcat aaatacgccg
     1561 gtcggactga aagttctgga gttcaactgc cgtttcggcg atcccgagac gcaggcgata
     1621 ctgcccagga tcgacttcga tctggcacga gttctgtacg attgcgccag aggccagctg
     1681 aaagaggagc ggctcaggct aaagcaggag tgcgccgtga cggtcgtggc ctgttcgaaa
     1741 gggtacccgg gccgttacgt aacgggttgc gaggtgtctg gactgaccga gggcaactgc
     1801 actctcttcc aagccggtac gggatacagc ggagagaaag tcgtaacgac gggcggcagg
     1861 gtgcttgccg tgacggcact ggagagagat tttggcgaag cggtgaggaa ggcttacggt
     1921 gcgctggaaa agatccgttt caaagggatg acattcagga aagacatagg cgtcgaattt
     1981 gatgtaaact agagtttgtc agcacgggag gttgattatg atcgaacgtt acgcactttc
     2041 gcccctgaag gagctctggg aactcaaagc ccagtacgag aggtggctgg aggttgagct
     2101 cgcggtggtg aaggcctacg aagagaccgg tcttgcaccg gccggaatgg cggaaaagat
     2161 aagatcgaag gctagaatag atgtcgaagg tatcgcagag atcgaaaagg tggtcgatca
     2221 cgacgtcatc gcctttataa aatacgtcac gggtgatatg ggcgacgagg cccgttattt
     2281 ccatctcggc ctcacatcgt cggacatagt cgatacggct ctttccttgg ctttgcgcaa
     2341 atcgggcctg gcgataatac aggctctgac ggagctttcg caggctctag ctgagcgggc
     2401 tctcatctac aaggatacgg tatgcatggg acggacccac ggaatacacg ccgagccgac
     2461 ctccttcggc ctgaagtttc tctccttcat ggtcgagacc gataggaata tcgagcgact
     2521 tcgcaggagt gtggaggcct cttcggtcgg aaagcttagc ggcgccgtgg gcaactacgc
     2581 caacataccg ccagaggttg aggctatagc cctcagggag ataggtctga gacctacgga
     2641 ggtatcgacg cagatcgtgc caagggatat ccacgccgaa ttcatctcgg ctatggcgat
     2701 tgcggcctct tcaatcgaga gaatggccgt cgagttcagg catcttcaga ggacggaagt
     2761 cctggaagtc caggaaccct ttaagaaggg gcagaggggt tcttcggcca tgccccacaa
     2821 gaagaatccc ataatctgcg agcgcttgac cggaatggcc cggcttctga gaggttacgc
     2881 cggcgcggcc ctggaagaca tagccctctg gcatgaaaga gatatctctc attccagtgt
     2941 cgagagaata atgttacccg acgccaccat gcttctgtac tatatggcga agaagtctat
     3001 cgatctggcg aaggatctgg tagtgtatcc agagaggatg ctggaaaact tcaaagcttc
     3061 cagaaacctg gtcttctccc agcgcgtaat gctgacgctg gtcgaaaagg gcatggcaag
     3121 ggaagaagcc tacaagcttg tccaggagct ctccctgaac tgctggaacg aaggccggga
     3181 cttcaaagaa gccgtcgcga aaagccgtga gatagaaaag tacattgatt cgcgcgaaat
     3241 agatgaactc ttcgatcctg agtactatct gagaaacgta gatccgatct tcaaaagaat
     3301 cttggacgga ggctgatcca atgcagagac tggccattgt gggagcccag tggggcgacg
     3361 aaggaaaggg aaaggtagtc aattacttct cgaaggattt cgagtggata gtcaggttct
     3421 ccggcggggc caacgccggt cacacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnncgaag gatttcgagt ggatagtcag gttctccggc ggggccaacg ccggtcacac
     3601 catatacgtc gatggcaaga aatacgtgaa ccatcttctg ccctcgatac agcccaggag
     3661 cgcttccagg ggctttctcg gggctggaat ggtcatagac gtggagcagc tcataaacga
     3721 gttcgagact ctggaagccg atttcccgga catctcctgc cggttctata tagatccgga
     3781 ggccttcgcc gttcttccct ggcacaaaga ggaggacgtg ctgctcgaga cgatgcgtaa
     3841 agagccgatc ggaaccacgg gcagggggat cggcccctca tacaccgata aagtgtcgcg
     3901 cgaaggtctg aaactctata cgctcttcga tgagaacctt ctcaaagaga ggctggaatc
     3961 gatctaccac atgaaacaga acatatacaa caacaggctc accgtatcgc ccgaggatat
     4021 gttcaaatac ctccagaggc tgcgcggtga actggaaaga ctgaaggtga acttcaccag
     4081 cgcggtcgat atgtcgcgcg tcttcaggag cacttcggtc ctcttcgaag gagcccaggg
     4141 cgttctgctc gacctcgatt tcggaactta tcccttcgtg acctccggag cctgcatggc
     4201 acacggcgtt tcgtcggtcg gtttctctac tttcgagcta gacagcgttt acggcgtgct
     4261 gaaggcctac actacaaggg tgggggccgg ccccttcccc accgaggact ttacagaaac
     4321 gggagagaag atacgcgaga tgggcaagga gtacggcgcc accaccggaa ggccgagacg
     4381 ggttggctgg ctcgacctgc cggcgttgag gtacgccagg atcagatcgg ggcttaccgg
     4441 attcgtcata accaagggcg atgtgcttaa cggactggaa gaagtaaaag tctgcgtggc
     4501 ttacgatgtc gatggagtgg tgaaggaagt accctctacc tcgtacgatt ttttcagggc
     4561 aaagccaatc tatgagacgg taaagggctg gcccacgacc gaccacataa acttcctgaa
     4621 gtacatgacc ttcatcgaga gagagaccgg cgtcgagatc gactatatct cctacggtcc
     4681 caaaaccgaa gagatgaaaa ccaggaacga cctgataata aatatttgat cccctgacgg
     4741 ggaaaggagg gaaggcagag catggatata cgactcaaca acacgatgtc gagagagaag
     4801 gaagtcttca aaccgctcac tgcgggggag gtgaaactct acacctgtgg attgaccgtt
     4861 tacaactacg cacatatagg caacttgaga gcctacatct ttgccgatac cctcaagagg
     4921 atgttcctct tcaacgatta caaagtgaac cacgtgatga atataaccga cgtcggccac
     4981 ctcacgggcg atgaagacga aggtgaagac aagatggagg ccggtgccag gcgagaagga
     5041 aagaccgtct gggagatcgt cgatttctac acgaaggctt ttttcaaaga tctggaaaga
     5101 ttgcggatac tcttccccac gaccacgtgc agagctaccg aacacgtggg cgacatgata
     5161 gaaatgataa aaaagataga ggccaacggc tacacttaca tcgccggcgg aaatgtctac
     5221 ttcgacacgt caaagcttcc cgattacggc aagcttgcca gacttcagct cgacgaagat
     5281 agaatgagat ctagggtgga aagcgatcca tacaaaagaa atcccttcga ctttgtgctc
     5341 tggttcaccc ggtacaaata caccgaccac gcgatgcagt gggattcgcc ctggggaagg
     5401 ggcttccccg gctggcacat agaatgctcg gccatgtcct ctaaatacct gggcgagagt
     5461 ttcgatatac acaccggcgg gatcgatcac atccccatac accacaccaa cgagatagcc
     5521 cagagcgagg cggccttcgg ccacgagtgg gtcaattact ggttgcactc ggagtttctc
     5581 gtgattggag agggtgaaaa gatgtcgaaa tcgctgggca acttcatcac cctccagacg
     5641 ctgatcgaca tgggatacga cccgatggat tacaggtatt acctgctcgg cgctcattac
     5701 agaaagcagc tggccttcac cttcgaagct ctcgacggcg cgaggaacgc catgaaaaga
     5761 ctcaagatcg gtatagctga gttgaaggag agtcaaagcc cttcagcctt cgccgacccg
     5821 caactgcttg aagagttcca cgaagccata aacgacgatc tcaacacgcc cagggccctg
     5881 gcggtgctct ggaaggttat cgacagcgaa gatctgtcgg ccggcgaaaa actcgcgctg
     5941 atagaacagt ttgacagagt tctggcgctc gggctggctg aagtcgagag cgatacgata
     6001 cccggagaga tcgaagagct cgcgctgcag cgtaaacagg ccagaaaaga caggaactgg
     6061 aaaagggccg acgaattgag ggacctgatc tcggagaaag ggtacgaaat cctcgacgac
     6121 cgtgatggat acaaaataag aaaaaagtga agaccggcgg cgaaagcatc gccccggtct
     6181 tccgttgtct ctgttcttta cagggccggc acccagatca ctggccggcc ctttctgggt
     6241 atttctttct tttcgaagat cattatatcg aagggtattt tgaaactcaa agggccgatc
     6301 gcagacttta ggctgccaag ggtgctgtcc cgcgcaaagt tgagcagagt cactttctct
     6361 ttccaggcct ctttgtggat ctccgcgacc actcttctgg cggcgcgggg agtcccgtaa
     6421 tctagaatga gcacatccca gatcctgagc ctttccttca ttcccgggac acgcgccaga
     6481 ggcgaaagga gggacctgcc tgctacctgg aaggctctca atccaggtga caacttcgct
     6541 atcccgaaat ccgactctcc cgaagtgtcg aagaccagcg cactggctcc ctctccggtg
     6601 aagagtttca acctctcctc ttctatcatg ggttcgaaga tacgcgtatg ctttggaacg
     6661 aggtcgaaac gaccggacat gccgctgtaa aggccgcgca ctctattcat gtcgggcctt
     6721 tccacgtaga ccccggtact tttgatgcga tgcagaacgg ggcaggcgta gaaatctagt
     6781 gtcgcgaagc gagaaaaacc catgtgcgcc agaatcttct tcgatgccgt gttgtctgcc
     6841 tttacggaag cgtaggcgaa atcgaactcg ctctccccgt acctgttctc gagttcctgt
     6901 attatagcct tcaaccagcg ggctatcttc atcctgtagg ccgggttggt tctcagcccg
     6961 aagaggtata tcgcctttct cggtacggaa tcgacaaggt actcgtcgaa ggctataccg
     7021 atacagccta ttatgtcgcc cttctcctcg gccaggagaa tggagccgtt accgtaatac
     7081 ttcagcctgt cgaagaagtt ttctttatag cctacgaccc agattttgcc ctcctgcgcc
     7141 gatctacgct cgatatccag cagtttttcg ttatcctctt ttgtggcgaa ccttatatcc
     7201 aatatacctc tccccgtcta ttttttgaga tgggtgtcga accattcaac aatggctttc
     7261 agtcgctttt cacgatgcac cggcattccg cccctggaga gttcgtggtt ttcacctggg
     7321 aagaggacca tcctggcctc tttgccgagg tacctcaggg tggtgaagag ctgcatcgct
     7381 tcaggcaccc agcacctgta gtcttccaga gaatgtatga acagtatggg cgtctcgacg
     7441 ttcggcgcgt aagtaagcgg cgactggcgc agatagcctt ccaggttctg gaagaagtcg
     7501 tcgcctacct ggtcgggtgc gaaatagtat cctatatccg tagttccgaa gaaagagatc
     7561 cagcttgata tcgaccgctg tgaaacggcc gccttgaaaa cgtcggtgtg gccgacgatc
     7621 cagttggtca tgaaaccgcc gtaggaaccg cccgtgacac ccagcctctc ctcgtctatg
     7681 aaatcgtact cattcacaac gaattccagg acttccataa tatcgtcgaa atcccgttcg
     7741 ccgtagtggc cccgtatatc tgcaaaatcc gtgccgtatc ccgagcttcc cctgggattg
     7801 cagtaaacta ccgcgtatcc cgaggctgcc agagcctgga actcgtgtat gtaaccgttg
     7861 ccataagccg tctttggacc tccgtgtatc tctactattg ccgggtatct gcccttttcg
     7921 tcgaagtcca ccggcttcat tatccagccg tcgatgacga caccgtcgga ggcctggatt
     7981 ttgaagcttt ccggcgccga tagctgatag cctttcatcc aggagttgaa attggttaat
     8041 tttttcgtgc cggtcgcctt gctgcagtat agctctagag gttgtaccgg cgtcatcttc
     8101 gtataaataa gatcgcctga atcgttgatg tcgaagcaat cgatgctccc gtcggagagc
     8161 ataacgtcct ccacttcacc tgacttatcc agtgagacga tcgtggagtc cggtcccgag
     8221 gttatgatcg agtatagctt tccctttttc accctgagag actctccaga caagcctcta
     8281 acatcgctgt tgagactgtt tccaaaatag aggtcgagat ccctgcgtag aggcttcagc
     8341 tctttgctct ccagatccac gaagttgaaa taagggttcg tcggaaagcc ctttgccaga
     8401 tcggtaccta gaacgaagag ttcgtcgctg gaggcccata cacaggcata cacggagatc
     8461 gaatcatctc cgatcctctc agtacttccg cctttcagat cgagaatata cagattgttc
     8521 cagcgcgggc gtctctcggc atcctctttg acaatgatgg ccgccctctt tctatcgggc
     8581 gagagctcga ccgattgaac atcgcccttg acggcttcca ggggtttcat cttgccggtc
     8641 tcgacgttca gagtgtgcag ttgagagatc ctgttttcgg tgaagcccga tccatttgaa
     8701 acaaagggga ttttgtctat cacgtagatt ctcttctcta ccggttcttc ctcggccggt
     8761 ttctccgggt tttcgccggg agccactttc gtggtgaaga gtatatttcc ctcgtcgacc
     8821 caggatatag acgagaaacc ccctttgaga gtcttaacga gcctgctttc acctccaccc
     8881 agctgcagaa ggtagagctc cgtgcctttc gcatctttct ctcttctgga cgtgaagagc
     8941 aaactcttgc cttcggggtc gaccacgggt gagccgtctt tatcgcccct ggtgagctgg
     9001 gtgaccttac cggtggagag gtcacccgac cagatgttgc tcaggtattt gttgttctcc
     9061 ctgtcgatgg tcttgtggac gaagagaaat tcccttccgt ttgggagaaa gtggacatcg
     9121 cctacggccg agtatctgtg aacgtcttcg agtcttatct tcttcaaacc aaccactcct
     9181 tgtcaaagaa atcttccgca gtctctcatc cagactgtac gttcgagtaa aatgatatca
     9241 atgatatcat tgtcttatag agattgcgag gtggagaaat ggttatcctg agtcattttg
     9301 accccttcgg cggcgacggg atcaacgcat ctaagatcgt cgcacagaaa gtggcgggta
     9361 tgaggtcaga tctgagggtg gtcgaactgc ccacggtctt cgacgacgcc ttcatccctt
     9421 tgatgacaga gatagagaga acggcgccgg aagcgattat catgctaggg caggctgccg
     9481 gaaggacggc cgtcactccc gagaaggtag cgataaactg gaaggaatcg acgaaggccg
     9541 ataacaacgg ctacatcgcg cggggcgaga gaatctcaat ctcaggcgcc gatgcttact
     9601 tttccacact tccgcttgtg gagataatcg actcgctcca gcgagatggt ctgcccttat
     9661 cgctctcatt cacggcaggc accttcgtct gcaactatct cttctacagg acgatggagt
     9721 acctggtctc gacgggcaaa gattttccgg tcggtttcgt gcacataccc tgcatccccc
     9781 agcagacggt tggaaagaac ggtctggcat ctatggacgc agccctgtcg gccgcaattc
     9841 tctcccggat gatagacgtg atcgtgggtg atggaagatg atcatcggcg gagcggcctt
     9901 agacatagcc tgggaggatc cggcaaaaaa catggaaaag atcgcggata tcttcgatca
     9961 ggcctttaaa aaacacgtgg agttgctggt ctttcccgaa atgtctctga gcggcttctc
    10021 cctgaatccc gaaaagacct gtacaccata cagcgatagc tttttcgatg gactggtgag
    10081 gaaaacttcg atcgcggccg tctacggtca cgtggagaga gaagagtccg gatatgtcaa
    10141 ctgcgcttcg ttcctatcta ccgatctggg acctgtcacc tacggaaagc gcaaactctt
    10201 cacttacgct gacgaacatg aaagctacgt tcccggcagg caggccatat ctctgattca
    10261 ccaaggcata gaaatatctc tcaacgtatg ttacgatctc cgcttccccg aacttttcag
    10321 ggagaacgtt ccggccgaac tgatggtagt gatagctaac tggccgtccg caagagccag
    10381 acactgggaa gtacttctaa gagccagagc ggtcgagaat caatgcttca ctctcggggt
    10441 aaacaggaca ggtagcgacg gaaagggagt ggagtacggc ggcacaaaat ccatgttgat
    10501 cgatcacaac ggagacgaag tcgaggtgga aaaggtcgga gagctgctta tatgggaatt
    10561 gaaagatgag gctgtggaaa gaatgagagc ctggagaagg aagttcccgg ccctcgaaga
    10621 tatgtgagcc cggttcaccg atcgcttctt ttttcgattt ggttgagtat ctccctttga
    10681 aaaaactcct cgacgttttc cggtgaaagc cggcaatgcc tctggccatc gacttccacc
    10741 accacaccct cagaacatgg gcccatgcag aaagaggcct tgaaatcgat actgtcggcc
    10801 agagccttct catcgatcaa ctcctgcaat cttttggcga ttttcgaagc tccaagaaca
    10861 taacacgcac ttcccataca gattcttata agcataggta cggctccagt tgtgattagt
    10921 ttcacaataa tattatattc tatcctgtcc gtttcccggt tgaagggtct gtaaagtact
    10981 tacttatcct acgggatttc tctgcgtgct tccaccgtga tattattctc acgaaaggtg
    11041 ggatcgttgt gaagatactc gtcgtggaag acgaacacga cctgggactg cttcttaaag
    11101 aggtcctcga gagtaacaac tatgcggcag agctctccgc agaaggggag gaagggcttt
    11161 acatggccct taacggcccc ttcgatcttg tggtgctgga tgttctcctg ccgggtctat
    11221 cgggctggga agtgcttgaa gggatgagac gaaagggctg tactacgccc gtactgatgc
    11281 tcaccgcgct agatagcgtc gataacaaaa taaggggttt caatctggga gccgatgatt
    11341 atctttccaa gcccttcgat atgcgcgaac tactggcgag gatacagtcg cttctgagaa
    11401 gaaccgcgcc cgtcgcggca cctacgaacg aactcgtttg cggagatctc gttctgaata
    11461 tatccgacaa gagcgtctat cggggctcga cgaagatcga actgagaaag aaagagtacc
    11521 agatactcga atacctgatg atcaacaaga ggagagtgat ctccaagagc gagctggaag
    11581 atcatatctg gaacgaggag gacgaactct ggtcggacgt tatccggagc catataaaga
    11641 acctgcgcaa gaagatcgat ggcggttaca gaaagaaact gataaaaacc gtgcggggta
    11701 tgggttatga aatcagtgaa gagtgaggag ctatccttta aaagcgcaag aatacgatgg
    11761 acaacatttt ttaccatagt cgtcgtttcg atagtctcga tcctgtgcgt gatagtcttc
    11821 tttttcgcgc tgcgatacga aaccaggtct tcttacgccg gtctcaacca gatggcagac
    11881 aggatagcca cgagaatagg cagggcaccc ctgataaccc tggagagaat tcttagaaac
    11941 tacgccgaga atcccgatct cttcaccgcc gagaacgaat a
//
LOCUS       DAOOLY010000047        11942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5942_length_11942_cov_4.906537, whole genome shotgun sequence.
ACCESSION   DAOOLY010000047 DAOOLY010000000
VERSION     DAOOLY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11942
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_11942_cov_4.906537"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLD15_03855"
     CDS             complement(<1..462)
                     /locus_tag="PLD15_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPB63068.1"
                     /translation="MDMESEFLLNWYERQLLVKGIDDRLQLKLSGETFGRLTGELEEL
                     VAELLIRSGLSGITKEFPRQEILPFYSPLIYRQEETVIFEDGPLLEIVVEKEKKRISF
                     PHSLPSAFAPIIAGRIFSWFMDGKYEKAFNMKLPAGLDRIGRPGKVMVVGAG"
     gene            complement(561..2291)
                     /locus_tag="PLD15_03860"
     CDS             complement(561..2291)
                     /locus_tag="PLD15_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63069.1"
                     /translation="MIRKFIAYYRPHLRLFILDMACAFMIAAIDLVFPTFTSRALDDY
                     IPSGNMRGIVIIAVVMALLFVLRAVFNYIVNYWGHVVGVRMEYNMRKDIFSHLQTLSF
                     SYFDRVRTGKIMSRIVNDLREITELAHHGPEDLLISLITLTGAFIILVQTDWRLTLVV
                     FAYIPFMIWFGVKKRQKMAQAFRIVRKKIANVNAQVENSISGIRVAQSFTNENFEKLK
                     FDMGNREFKESRQFAFKSMAEMTTGIDLLMNLLKVTVLALGGYLTYAGEITVGSFVAF
                     FLYIELFLQPIRRLMQFAQQYEDGMSGFERFIEIMETKSSVTDSPNAVELKDVVGEVK
                     VENVTFAYDGGENVLKNITLDIPAGKMIALVGPSGGGKTTLCHLIPRFYDVKDGRITI
                     DGKDIRDVTLNSLRRNIGIVQQDVFLFAGTVRDNIAYGKGDATDEEIIEAATKANIHD
                     FIMTLDKGYDSYVGERGVMLSGGQKQRISIARVFLKNPPILILDEATSALDNETELKI
                     QASLEMLSRGRTSLVIAHRLSTIKNADEIIVITADGIIERGSHSELLKLSGHYARLYK
                     AQFKGYIPDM"
     gene            2499..3155
                     /locus_tag="PLD15_03865"
     CDS             2499..3155
                     /locus_tag="PLD15_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63070.1"
                     /translation="MTGTKTTVSHTDRSAFGKNSEVTEPSENRLEELFAERKGENVGE
                     VVITGREFVRENLPQSMTVEHFIEKYNSWYSQGTIEIRFRDVTRTRHYTMQSDRRLSL
                     DELSYFMKTWKDEGLDRILFYETRTESDIPTGWNETAPEDLKSLKPREGEGKFSTRQR
                     IEPLQKVLVLRMSDEYGVRVTVLRSIATGGKVPQNRRAAGDAIVGERAIRNAGNMEIN
                     "
     gene            3328..4617
                     /locus_tag="PLD15_03870"
     CDS             3328..4617
                     /locus_tag="PLD15_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPB63071.1"
                     /translation="MNSMDKLFVGIYFILAFTLIVFSKKRRVLLAFTAGMLLVVLKVS
                     ESMRIETISEFVDFNAIFLLIGMMVIVAVIKSTGFFQYIAIRTLKWTRGNIMLLSALF
                     AALIALFSMILDNVTTMIMFMPVIFFVADGAGFNPFAFTTVMILASNVGGCMTLVGDP
                     PNIIIGNASKIPFLTFTALVFIPLAISFVVLLLMSRFSILKSLATVSQNNSVIKEMRT
                     EGVITNPRLMIVSLSTLVIVVIGFIIHSVVDIEMSLFAILGAAFLLLYTGKDFDHMAN
                     EVDWNAILFFIGLFSLAYSLESSGIAGDLSRIAMKLEGNPILLSSAILWLSGFIAMIT
                     GAIPVVTIFIPIVANLSAIYPLPYDLWIALALGANLGGNGTLTGHLANIITFEMLNKE
                     ARKKNTFLDFLKLGFPFSVVSLVISNLYLIVRLLMNG"
     gene            complement(5335..6300)
                     /locus_tag="PLD15_03875"
     CDS             complement(5335..6300)
                     /locus_tag="PLD15_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB63072.1"
                     /translation="MPDTLIKIENLKTYFPVKKSILSKKLFVRAVDDVTMEIPRGSTF
                     AVVGESGSGKTTLARSVLRLIEPTSGKIFFDGSEILRLKGKDLLDVRRKMQIVFQDPY
                     NSLHPRKLIKNIIGEGMKIHFKITENEIRDKISAVLKEVGLRDEHMFRYPHEFSGGQR
                     QRIAFARAIVLKPKFIVLDEPTSALDVSVQAMVLKMLKDIRAQEDLTYMFITHNLSLV
                     DYIADRVAVMYVGEVVEMGEKNTVFNHPSHPYTDLLMASNPIPDPKFQREKRLLKGEI
                     PSSINPPSGCRFHNRCPHADQKCASQAPEMREHAPGHFVRCHYPL"
     gene            complement(6293..7285)
                     /locus_tag="PLD15_03880"
     CDS             complement(6293..7285)
                     /locus_tag="PLD15_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63073.1"
                     /translation="MELRGKLLDVSGLKVSFYTYRGVIKALDGVELWMDSGERLGVVG
                     ETGCGKSVTSLAVMRLIEQPGEIQAGKVIFNGKSLLELSEENMNRVRGIEMSMIFQEP
                     RSSLNPVMKVGYQIGEAIAKSKKMPIKKTYPEVREMLKLVGLDPDRVMNSYPHELSGG
                     MSQRVMIGMGLATHPKLLIADEPTSALDVTIQAQILELLDELARKMGNAVMLITHDMG
                     VVAEFCDKVMVMYAGNSVEYASTVELFERPLHPYTGGLLLAVPRVGRTDELKAIKGTV
                     PDLVDPPSGCRFHPRCPRVMEICKENRPPLVQVEPGHFVACYLYGNEGEVKENA"
     gene            complement(7290..8162)
                     /locus_tag="PLD15_03885"
     CDS             complement(7290..8162)
                     /locus_tag="PLD15_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63074.1"
                     /translation="MEKTAKVSRPFMDDLKHTAFLWRRSRLTMIGSVIIVIFLLIAAF
                     APLIAPYSPVEQNLSIKLQSPSWQHLFGTDQFGRDIFSRVITGSRTALWIIFLVSVIS
                     GSIGIIIGVTAGYFGGIVDEVLMRITDMFLAFPSLVLAMAFAAMLGPNLTNTIIAISI
                     VSWTTYARLSRAEAIKVKSQPYIEAIRAAGGGNLRIMFLHVLPMCISPVLVQLTLRMG
                     TIILTAASLGFLGLGVQPPTPEWGAMVSDGRNFLIDQWWISTFPGIFIAFVVLGFNLL
                     GDGIRDMLDPRLRR"
     gene            complement(8168..9178)
                     /locus_tag="PLD15_03890"
     CDS             complement(8168..9178)
                     /locus_tag="PLD15_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63075.1"
                     /translation="MNFGQFLLRRLFLMIIVLFGVACIVFFIANIIPADPVGAILGGN
                     APPEAINRLKAKLGYDQPLIIRFGNFIAGALRGDFGVSLKTSNPVMKDIMNYFPATME
                     LAIVAIIISIFLGITLGVLSAVHRNKAIDQFSRIFSILGVSMPVFWIGLLLLLVFYFK
                     LGWLPGGGRLGFFTYPPPRVTGMYLIDSALAGQWDTFKEALLHILLPAFVLGYNATAS
                     IARITRASMLDVLKQDFIRTAKSKGLKRNVVIYRHALRNSMIPTITIIGLVFGGLLEG
                     AVLTETVFSWPGLGRYITTGMLFLDYPAVMGGTLYIAFIYSLANLIVDILYALLDPRM
                     RM"
     gene            complement(9254..10825)
                     /locus_tag="PLD15_03895"
     CDS             complement(9254..10825)
                     /locus_tag="PLD15_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63076.1"
                     /translation="MKRSFMLVFLTVLIVAVTGFAATPKDTLVIGSNTGIFITMDPAV
                     AYEVLPNKIVAACYAQLVNLKAIDGVIVPVPDIAESWEISEDGLTYIFHIRQGVKFSN
                     GDPLTAEDVLFSLKRPILLDSVSAWFLVDIFGINKDNVDEKIKQIDENTITITLNAPY
                     AENITLGILSNMFTGIVNKDVVLANEVNGDLGTAWLADHSAGAGPYVLVQWERNNVIL
                     LEANPNYYGSQPPLKRIMIRDVPEASNQRLFLERGDIDVAWDLTPQLLTEAKKNPNVV
                     EVKVPGHSNEYLAMNASWGPLKDPKVREAVRYSINYKEIVEDIMLNNAMLVQGFINKG
                     YFGYVEENPFYQDIEKAKALLAEAGYPNGFEVELITSNTDTRKAEAEKIQADLAKAGI
                     KVNLVIVQSSQMYEKYRAQGHQMIIAGWGNDYPDPDNLAMSHASYRANQLAWRNAWYD
                     DYAATLCEWGQIEPNQDKREQIYKDLTEYWFHNGPFAMLYQTVEFWGIRKEVKNFEEA
                     AFGYGMLFDFTKVSK"
     gene            complement(10878..11912)
                     /locus_tag="PLD15_03900"
     CDS             complement(10878..11912)
                     /locus_tag="PLD15_03900"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103031 - L-Ala-D/L-Glu epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HPB63077.1"
                     /translation="MKISDLRFTERKWDFVKPFHIANSVSSSKVNIEVELVLADGTIG
                     LGESSSSFRVNGESNESIFQLRAEMLETIKGLDVKDYRRVFKKLDAYSRSAPSLKAAV
                     QFATLHAFSRLISTPVYQILGGMKDFVETDKTVSIGSLEETVNDAREIFNAGHKVIKM
                     KVGEDLQSDIARVLAVNDEIKGVKYIIDANTGYTPKEALRFIDAMYRNSIPVGIFEQP
                     VPAEDFDGLKIVRQGSMYPVGADESARTKFDVMRLIKEGAVDYVNIKLMKSGISDALA
                     IVEMCNSAGIGLMIGCMSESGIGVSQSVHFAAGTGAFMYHDLDSHLLLKNVEPVGFRQ
                     ERDRQYPILF"
BASE COUNT         2829 a         3085 c         2977 g         3051 t
ORIGIN      
        1 gccggcaccg accaccatta ctttgccggg ccggccgatg cgatcgagcc cggccgggag
       61 cttcatattg aaggcttttt cgtacttgcc gtccataaac cacgagaaga tcctgcccgc
      121 gattatcgga gcaaaggccg acggcagaga gtgggggaag gagatccttt tcttctcctt
      181 ttcgacgaca atctcgagaa ggggaccgtc ttcgaagata acggtctctt cctgcctgta
      241 tataagcgga gagtagaagg ggagtatctc ctgcctggga aattccttgg ttattccgga
      301 aagaccgctc cttattagca actccgccac gagctcttca agctctccgg tgagccttcc
      361 gaaggtctcc cccgatagct tcaactgtaa tctgtcgtcg atccctttca ccaggagctg
      421 tctctcgtac cagttgagca agaattcact ttccatatcc atgactcatc cctcccgtgg
      481 atcgaaacac aacacaagta tatcagctgt gtcctcacag gagagtccgt tcgaggggtc
      541 acgaaacgtg gcgcctgttt ttacatatcc ggtatgtagc ccttgaattg agctttgtag
      601 agtctggcgt agtggcctga gagtttcaac agttcggaat ggcttcccct ctctattatt
      661 ccgtcggcag tgatgactat tatttcatcc gcgttcttta tagtcgataa tctgtgggct
      721 atgaccagag aggttcttcc cctggagagc atttcgagag aggcctgtat cttcaattcg
      781 gtctcgttgt cgagggcaga agtcgcctcg tccaggatca gaatcggagg gttcttgagg
      841 aagaccctgg cgatagagat cctctgtttc tgtcctccgg agagcataac gcccctctct
      901 ccgacgtagg aatcgtaacc tttatccagc gtcattatga agtcgtgtat gttggccttc
      961 gtggccgcct cgattatctc ttcgtctgtc gcgtcgccct ttccgtaagc tatattgtcg
     1021 cggaccgttc cggcgaatag gaagacgtcc tgctggacta tcccgatatt ccttcgcagc
     1081 gaattcagtg tgacatccct tatatccttg ccgtcgatcg tgatccttcc gtccttaaca
     1141 tcgtagaacc gcggtatgag gtggcagagc gtggtcttgc cccctccaga aggcccgacc
     1201 agggcgatca tttttccggc cggtatatcc agggttatgt tcttcagaac gttttctccg
     1261 ccgtcgtagg cgaaagtgac gttctcgacc ttaacctctc caaccacgtc tttgagttcg
     1321 acggcgttcg gactgtcggt tactgaagac ttggtctcca ttatctctat gaatctctcg
     1381 aaaccggaca ttccgtcctc gtactgctgg gcgaactgca tcagcctcct gataggctgc
     1441 aaaaacaatt ctatataaag aaaaaaggcc acgaacgaac cgaccgtgat ctcgccggcg
     1501 tacgtaagat acccacccag agccagcacc gtaaccttca gcaggttcat cagaaggtct
     1561 atgccggtcg tcatctcggc catagacttg aaggcgaact gccgggactc tttgaactcc
     1621 cggttgccca tatcgaattt tagtttctcg aagttctcgt tggtgaagga ctgggccacc
     1681 cttattccgg agatgctgtt ctccacctgg gcgttgacgt tggctatctt ctttcgcacg
     1741 attctgaagg cctgagccat cttctgtctc ttcttcacgc cgaaccatat catgaaggga
     1801 atgtacgcga aaaccaccag ggtcaatctc cagtcggtct gcaccagaat gatgaaagca
     1861 cccgtcaggg tgatcaagga tatcagcagg tcctccgggc cgtgatgggc aagttcggtt
     1921 atctctctga gatcgttgac gatcctcgac attatctttc ccgttctcac cctgtcgaaa
     1981 taactgaagg agagagtctg aagatgggag aagatatcct tcctcatgtt gtattccatg
     2041 cggaccccga cgacatgacc ccagtagttc acgatgtagt tgaacacggc cctcaatacg
     2101 aagagcaagg ccataaccac tgcgatgatc actatccctc gcatatttcc cgaagggata
     2161 tagtcgtcga gcgccctact cgtgaaagtc ggaaagacca gatctatggc cgcgatcatg
     2221 aaggcgcagg ccatgtccag aatgaacagc ctcaagtgtg ggcgataata ggcgataaat
     2281 ttgcgaatca ttcagagcct ccattaaagt aaggtaagcg tctcaatttt actccatcgc
     2341 cagaagtata acaagcaagc aaaggaaatc cgaaacatcg cccgcatggg tatcactttc
     2401 cacataaaat cccacaatct cccaaattct cactgtattt ctcccaaatt catttactag
     2461 aatgggtttg aaagataaag acagcaggag tgatgaaggt gacgggaacg aaaacaacag
     2521 tttcacatac agacagatca gcctttggaa agaacagcga agtgacagaa cccagcgaaa
     2581 atagattaga agagctgttc gccgaacgaa aaggagagaa cgtcggggag gtggtcatta
     2641 ctggaagaga gttcgtcaga gagaacttgc cacaatcgat gaccgttgag cacttcatcg
     2701 aaaaatataa cagctggtac agtcagggaa cgatagaaat aagatttcgg gacgtaacca
     2761 gaaccaggca ttacacgatg cagagcgaca ggagactttc tctggatgag ctgagctatt
     2821 tcatgaaaac atggaaagat gaaggtcttg atagaattct cttttacgag acaagaactg
     2881 agagcgacat tcctaccggc tggaatgaaa cggctccaga agatctgaaa agtctcaaac
     2941 cccgggaagg tgaaggaaaa ttctcgacaa gacagagaat agaaccattg cagaaagtgc
     3001 tcgtccttag aatgtcggat gaatacggtg tcagggttac ggttctgaga agcatagcaa
     3061 caggcgggaa ggtaccacaa aacaggagag cggcaggtga tgctattgtt ggagaaagag
     3121 cgatccgcaa cgcagggaac atggaaatca actgaatact ttatcccccc caattgaaag
     3181 tgaagtgagc aagtgcggaa cctgaaagtg gaaacaacac agcaactgtc acagaacgcc
     3241 atttagactt catagaacct ccaataaccc gggccagacg gcccgggctt catatgggtt
     3301 ttgcaaagag cgcgctatgg ggttacaatg aacagtatgg ataaactctt tgtcggcatc
     3361 tatttcatac tggccttcac gctcatagtc ttttcgaaga agcggcgggt tttgctcgcc
     3421 ttcactgccg gaatgttgct ggtagtgctg aaagtctccg aaagcatgcg catagagacc
     3481 atatccgagt tcgtcgattt caacgccata tttttgctta taggcatgat ggtgatagtg
     3541 gcggtaataa aatcgaccgg cttcttccag tacatagcca tcaggacctt gaagtggacg
     3601 cggggcaaca tcatgcttct ctctgccctg ttcgccgccc tgatcgccct cttttcgatg
     3661 attctggaca acgtcaccac catgataatg ttcatgccgg tcattttctt tgtcgccgat
     3721 ggagccggct tcaacccctt cgccttcacc acggtcatga tactggcctc caacgtgggc
     3781 ggctgcatga cgctcgtggg agatccgccg aacataatca taggaaacgc atcgaagata
     3841 cccttcctca cgttcacggc cctggtcttc attcccctgg ccatatcgtt tgtcgtgctc
     3901 ttgttgatgt ctagattcag catactcaag agcctggcta cggtctcgca aaacaactcg
     3961 gtaataaaag agatgcggac cgaaggcgtg ataaccaacc ccaggctcat gatagtctcc
     4021 ctatctaccc tggtgatcgt tgtgataggc ttcataatcc acagcgtcgt cgatattgaa
     4081 atgtcgctct tcgccatact cggcgcggct tttctgctac tgtacaccgg caaggacttc
     4141 gatcacatgg caaatgaagt tgactggaac gcgatactct tcttcattgg tctcttttcg
     4201 ctcgcttatt ctctggaaag ctcggggatc gccggcgatc tctcccggat cgccatgaag
     4261 ctcgaaggca acccgatcct tctctcaagc gcgatcctgt ggctcagcgg tttcatcgcc
     4321 atgatcaccg gcgccatacc cgtcgtgacg atcttcatac ccatcgtggc caatctctcg
     4381 gccatatacc cgctgcccta cgatctatgg atagccctgg ccctgggcgc gaatctcggt
     4441 ggcaacggta ctctcaccgg tcatctggcc aacataataa ccttcgaaat gctcaacaaa
     4501 gaggcccgaa agaaaaatac ttttctggac tttctgaagc tgggcttccc cttttccgtg
     4561 gtttctctcg tcatctcgaa tctctacctc atagtcagac tgctcatgaa tggataaaag
     4621 tctctaaaat ttaggaaatg gacttccgat ttcgtgaaat gaatttccac gacgaaattt
     4681 ttgggaaatc tatttccaaa acaccggctt tttcgggaaa tacatttcct tttttcggag
     4741 agttatgaac attgaatgtt cataactcgg tcttaaatct ctgtaagcct tgtttgaagg
     4801 caattacagc gatgttcata actcaaatcg acgtatctgg ctatgggaaa cgattatcgc
     4861 atattgattc gtatagcaaa ctgagttatg aacatgcccg tcgagttatg aacatgtgcc
     4921 atgtagtaaa aaacgtctta attcagctcc agaaaacaaa tacaggcgtt ttgagttttc
     4981 tacattctat aatcggccat tcatggtcca tgcgtgagtt atctacattt aatgtagata
     5041 acttttgagt tatgaacatg ggtcaaattc gcttccacaa aagctttgac tgagttttct
     5101 acgagttttc tacgaagatt attattatta tttatttata tatcaagaaa gaaagaaatt
     5161 aagagcctac ccagtaaaaa gcaagagatt caaatctaca catcaagata cagagtccgg
     5221 taagaagtag attgagctta gaaaacatga atagcttctc atagtacaaa aaagcaggga
     5281 cagaatcttg atacctgtcc ctgctttcag gagtctgata caaaaaccaa ctgctcaaag
     5341 agggtaatga caccttacga agtgtcccgg agcatgttct ctcatttcag gagcctgact
     5401 ggcacatttt tgatcggcat gagggcacct gttgtgaaac ctgcacccgg aaggcggatt
     5461 tatcgagctt ggaatttcgc ccttcagcaa ccttttttcg cgttgaaact tcgggtcggg
     5521 tatcgggttg gaagccatca ggagatcggt gtacggatgg gacggatggt tgaaaacggt
     5581 gtttttctcg cccatttcga cgacttctcc cacatacatg acggcgaccc tgtcggctat
     5641 ataatcgacc agcgagaggt tgtgggttat gaacatataa gtcaggtctt cctgcgccct
     5701 tatgtccttc agcatcttca aaaccatcgc ctgcacagaa acatccagag ccgaggtggg
     5761 ctcatccagc actatgaact tcggcttcag gacgatggcc ctggcgaagg ctattcgctg
     5821 gcgctgtccg ccggaaaact cgtgaggata gcggaacatg tgttcgtccc tgagaccgac
     5881 ctctttcaac acggccgaga tcttgtccct tatttcgttc tcggtgatct tgaagtgtat
     5941 cttcattccc tctcctatga tgttctttat gagctttctt ggatgcagag agttgtatgg
     6001 gtcctggaag actatctgca tctttctcct gacatccaga aggtccttgc ctttcaatct
     6061 gaggatttcc gagccgtcga agaagatttt ccccgaagtc ggttctataa gcctcaagac
     6121 gcttcttgca agcgtggtct ttcccgagcc ggactcgcct acgacggcga atgtcgagcc
     6181 ccgtgggatt tccatcgtga cgtcatccac ggccctcaca aagagtttct tcgagagtat
     6241 cgacttttta accgggaagt aggtcttcag attttctatc tttatgagag tgtcaggcat
     6301 tttccttcac ctcgccttcg ttgccgtaga gataacaggc cacgaaatgt cccggttcca
     6361 cctgaacgag aggcggtctg ttttctttac agatctccat gacgcgggga catctgggat
     6421 ggaacctgca accggaaggc ggatccacca gatcgggcac ggttcccttt atcgccttga
     6481 gctcgtcggt cctccccact ctcggcaccg ccagcagcag tccccccgtg tagggatgga
     6541 gcggcctctc gaagagctcg acagtcgagg cgtattcgac ggagttaccg gcgtacatca
     6601 ccatgacttt gtcgcagaat tcggcgacga cgcccatgtc gtgggtgatg agcatcacgg
     6661 cgtttcccat ctttcttgcc agctcgtcga ggagttccag tatctgcgcc tgtatcgtaa
     6721 cgtccagcgc cgaggtcggc tcgtctgcga taagtagctt cggatgggtg gctaggccca
     6781 ttcctatcat gactctctgg gacatgccgc cgctgagttc atggggataa ctgttcataa
     6841 ccctgtcggg atcgaggccg accagtttga gcatctccct gacttcggga tacgttttct
     6901 ttatgggcat tttcttcgat ttcgctatgg cctctccgat ctggtatccc actttcatga
     6961 cagggttgag cgacgaacgt ggttcctgga agatcatcga catctctatg cctctcaccc
     7021 ggttcatgtt ctcttcggag agttcgagga gcgacttgcc gttgaaaatc actttgccgg
     7081 cctgtatctc gccaggctgt tcgatcaatc tcattaccgc cagcgatgtg acggactttc
     7141 cgcagccggt ttcgccgacc acgccgagac gttctccgga atccatccac agttccacac
     7201 cgtcgagcgc ctttatgact cccctgtaag tgtagaaaga gactttcagg ccggacacgt
     7261 cgagcagttt gcctctcagt tccatacggt cacctcctca acctgggatc cagcatatct
     7321 ctgattccgt ctcccaggag gttgaaaccg agaacaacga aggctatgaa tatacccgga
     7381 aaggtcgaga tccaccactg gtcgatcaaa aagtttcggc cgtcggaaac catagctccc
     7441 cattccggcg tgggtggctg cactcccagc cccaggaagc cgagactcgc agcggtcagt
     7501 attatggtgc ccatcctgag cgtcagttga acgagaacag gcgaaatgca catgggaaga
     7561 acatggagga acattatgcg cagattgcct ccgccagctg cccttatggc ctcgatgtac
     7621 ggctgggact tgacttttat cgcttcggcc ctcgacagtc tcgcgtaagt ggtccaggaa
     7681 acgattgaaa tcgctatgat cgtgttggtg aggttaggcc ccagcatggc ggcgaaagcc
     7741 attgccagca ctaggctggg aaaggccagg aacatatcgg tgatcctcat gaggacttca
     7801 tcgacgatac ccccgaaata cccggccgtc accccgatga ttataccgat cgaaccgctt
     7861 attaccgaaa cgagaaatat gatccagaga gccgttctcg atcctgtgat gactctgctg
     7921 aaaatatccc tcccgaactg gtccgttccg aagagatgct gccagctcgg cgattggagc
     7981 tttatcgaaa ggttctgttc gactggactg tatggcgcta tgagtggggc gaaggctgca
     8041 atcagcagga atatcactat tattaccgac cctatcatcg tcaggcggct tcttctccag
     8101 agaaaagccg tgtgtttcag atcatccatg aaaggtcggg atactttggc tgtcttttcc
     8161 attcagatca catcctcatc cttggatcga gaagcgcgta aagaatatcc actatcaggt
     8221 tggccaggga gtagatgaag gctatataca gcgttccgcc catgactgcg ggatagtcga
     8281 ggaagagcat tccagtcgtt atgtatctgc ccagtcccgg ccaggaaaaa accgtttcgg
     8341 tcaggaccgc tccctccaga agacctccga agaccagccc tattatggtt atggtgggta
     8401 tcatagagtt tctgagggcg tggcggtaga taacgacgtt tctcttcaaa cctttggact
     8461 tggccgttct tatgaagtcc tgcttcaata catccagcat gctcgccctg gttattctcg
     8521 cgatcgacgc cgtggcgttg tagccgagga cgaaggcggg aaggagaatg tggagcaacg
     8581 cttccttgaa agtgtcccac tggccggcga gggccgagtc gataaggtac attccggtta
     8641 ctcttggagg cggatatgtg aagaaaccca gtcggccgcc gccgggaagc caccccagct
     8701 tgaagtagaa aaccagcagc agcagcagcc ctatccagaa gacaggcatc gagactccca
     8761 gtatggagaa gatcctcgag aactggtcta tcgctttatt tcgatggacg gccgaaagga
     8821 cacccagcgt tatcccaaga aagatcgaga ttattatcgc gacgatggct agctccatcg
     8881 tggcggggaa atagttcatg atgtctttca tgaccgggtt gctggttttc aaagacactc
     8941 cgaagtcgcc acgcaacgcg cctgcaatga agtttccgaa ccgtatgatt agaggctggt
     9001 cgtaccccag tttggccttc agtctattta tggcttctgg aggggcattt ccaccaagga
     9061 tcgcacccac gggatcggcg ggtattatat ttgctatgaa gaagacgata caggcgaccc
     9121 cgaatagaac tatgatcatc aagaatagtc ttctcagtaa gaattgtccg aagttcacag
     9181 aattaccccc aatagagact ttagattttt atgaaggata acgccccgct cagagagcgg
     9241 ggcgggggat attttactta gaaaccttgg tgaagtcgaa cagcattccg taaccgaacg
     9301 ccgcttcttc gaaattcttg acttccttgc ggattcccca gaattcgacc gtctggtaga
     9361 gcatggcgaa cggtccgttg tggaaccagt attcggtcag gtccttgtag atctgttcac
     9421 gcttgtcctg gttgggctct atctgacccc actcgcaaag ggtggctgcg tagtcgtcgt
     9481 accaggcgtt tctccaggcg agctggttgg ccctgtaact ggcgtgagac atggccaggt
     9541 tgtccgggtc gggatagtcg ttgccccagc cggcgatgat catctggtgg ccctgtgcgc
     9601 ggtacttttc atacatctga gaagactgca cgatgaccag attcactttt atgccggcct
     9661 tcgcaagatc ggcctggatc ttttcggcct cggcctttct ggtatcggtg ttgctcgtga
     9721 tcagctcgac ctcgaatccg ttgggatatc cagcttctgc gaggagcgcc ttggcctttt
     9781 ctatatcctg gtagaaggga ttctcttcga cgtagccgaa gtatcccttg ttgatgaagc
     9841 cctgcaccag cattgcgttg ttgagcatga tgtcctcgac gatctctttg tagttgatgg
     9901 agtatctgac ggcctctctt accttcggat ccttgagcgg tccccagctg gcgttcatgg
     9961 ccagatactc gttagagtga cccggaacct taacctcgac cacgttggga ttcttcttgg
    10021 cctcggtgag aagctgcggc gtgaggtccc aggcgacgtc gatgtcgcct ctctcgagga
    10081 agagtctctg gttggaagcc tcggggacgt ctcttatcat tattctcttc agaggcggct
    10141 gagagccgta gtaattgggg ttggcctcga gaagaatcac gttgttcctc tcccactgca
    10201 ccagaacgta tggaccggcg ccggcggaat ggtcggcgag ccaggccgta ccgagatcac
    10261 cattgacttc atttgcgaga actacgtctt tgttcacgat cccggtgaac atgttcgaga
    10321 gtatgccgag ggttatgttt tcggcgtacg gagcgttcag ggttatcgtt atggtgtttt
    10381 cgtcgatctg tttgattttc tcgtcaacgt tatctttgtt gatcccgaaa atatcgacga
    10441 ggaaccatgc cgagacggag tcgagaagga tgggtctctt caaagagaag agaacatctt
    10501 cggcagtcag gggatccccg ttggagaact tgacgccctg ccttatgtgg aagatgtagg
    10561 tgagtccatc ttcggagatt tcccagctct cggcgatatc gggaaccgga actatcacgc
    10621 cgtcgatggc tttgagattg acgagctgag cgtagcaggc ggcgacgatc ttgtttggaa
    10681 gaacctcata ggcaactgcc ggatccatgg ttatgaagat accggtgttg gagcctatca
    10741 ccagagtatc cttcggtgtc gcggcaaatc ccgtgactgc aacaatgagt acagttaaga
    10801 aaactagcat gaagcttcgc ttcattcttg cacctccttt tttcccacga aataagggtg
    10861 acaacagaaa tggaacacta aaataagatt ggatattgcc tatctctttc ctgtctgaaa
    10921 cctaccggtt ccacgttctt cagcagcagg tgggaatcga gatcgtggta catgaaggcc
    10981 cccgtgccgg ccgcaaagtg gacgctctgc gagacgccga tccccgattc gctcatgcag
    11041 cctatcatca gtccgattcc agcgctgtta cacatttcca ctatcgccag agcatctgag
    11101 atacccgatt tcataagctt gatatttacg taatccaccg cgccttcctt tattagacgc
    11161 atgacatcga acttcgttcg cgcgctttcg tcggcgccca ccgggtacat cgagccctgt
    11221 ctgacgatct tgagtccgtc gaaatcctca gccggtactg gctgttcgaa tatgccgacc
    11281 ggaatgctgt ttctgtacat cgcgtcgatg aagcgcaggg cttctttcgg cgtgtagccg
    11341 gtgttggcgt ctattatgta tttaacgccc tttatttcgt cgtttaccgc gagcaccctg
    11401 gcgatatcac tctgaagatc ttccccgact ttcatcttta taactttgtg accggcgttg
    11461 aagatctccc tggcatcgtt gacggtctct tccaggctcc ctatgctgac agtcttgtcg
    11521 gtttcgacga aatctttcat ccctccaagt atctggtaga cgggtgtgga aatcagtctg
    11581 gagaaggcgt ggagggttgc aaactgaacc gcggccttca aactaggggc gcttctcgaa
    11641 taagcgtcga gcttcttgaa taccctgcgg taatctttca cgtccagtcc cttaattgtc
    11701 tccagcatct ccgcacgaag ctggaagatg ctttcgttgc tctctccgtt cactctgaac
    11761 gaagaggatg actcacccag accgatcgtg ccgtcggcga gaaccaattc aacttcgatg
    11821 tttactttag agctggatac gctgtttgcg atgtgaaaag gttttacgaa gtcccatttc
    11881 ctttctgtga aacgaagatc gcttattttc atttgctcct cccgattaaa gaaagacttt
    11941 ga
//
LOCUS       DAOOLY010000048        11890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_5977_length_11890_cov_4.746599, whole genome shotgun sequence.
ACCESSION   DAOOLY010000048 DAOOLY010000000
VERSION     DAOOLY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_11890_cov_4.746599"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLD15_03905"
     CDS             complement(<1..329)
                     /locus_tag="PLD15_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPB63078.1"
                     /translation="MSILYKELSNNATLVGERKEETRTVSMAFAVKVGSADEDDPISG
                     VSHFIEHTLFKGTKDRNAFEIKEPIERIGGSLNAYTGRISTVYYAKVPDTYSHEALDI
                     LFDLISSP"
     gene            complement(340..2586)
                     /locus_tag="PLD15_03910"
     CDS             complement(340..2586)
                     /locus_tag="PLD15_03910"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPB63079.1"
                     /translation="MREFYATVIIEYLRIEKEVLLVSYKQWEREFFGQKLVIENGKMA
                     KQAHGAILLRYADSVLLTTVDGNEEAMPGTDFLPLTVEYQEKFYAAGKIPGGFLKREN
                     RPSDNAVLSARIIDRPIRPLFQDGMRNEIQVIVTVLSADPDNPPDIWGIMGASLALNL
                     SEIPFEGVVAGVQVGYIDGKYVIFPSAEELKRSELDIVVAGTKHAVTMVEGEAKEVSE
                     KIMVGALEAAHEAIKSLVVFQEEILAEFEIKKWELEIPTAPEGFVEAFGEFVDRDKLA
                     GLMLTPGKHAKDKALKEYRDQLIEAFVQKVNDRWSEEEIMGNIGYLKDSYHDVEKEVM
                     RRRIVQDDVRMDGRKHDEVRPITIELDLLPRAHGSALFTRGETQSLGIVTLGATMDEQ
                     IVDTMFEEGSKSFMLHYNFPPFSTGEVKRLRGPGRREIGHGHLAERSLKNIVPKDEDF
                     PYTIRVVSEILESNGSSSMATVCSGSLALMAAGVPMKKHVAGVAMGMIQEPDKTVVLT
                     DILGNEDHMGDMDFKVTGTRDGITAFQMDVKVSGVSGDIMTQALEQAKVARLKILDLM
                     YRAVDTPRTTVSDYAPIIKTIALPYEKIGEIIGPGGKVIKKLSSDYDSTIFIDDEKSL
                     AKIVGNDHAKLERLEKVIEAIISEAKPGQIFEGKITREEAYGFFVELAPGKTGLLHVS
                     KMGENSKEFLKAHKLGDIIKVEVSGIDQMGKISLKLEGVEVTEEQRRERKPYPRNEGR
                     NRNERNRK"
     gene            complement(2629..2889)
                     /gene="rpsO"
                     /locus_tag="PLD15_03915"
     CDS             complement(2629..2889)
                     /gene="rpsO"
                     /locus_tag="PLD15_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB63080.1"
                     /translation="MEINKQELVKEFQIHDNDSGSTEVQIAILTARIRHLTEHLKKHP
                     KDFHTRRGLLKLVGRRRKMLRYIKAKKPEVYLELINKLGLRG"
     gene            complement(2970..3842)
                     /locus_tag="PLD15_03920"
     CDS             complement(2970..3842)
                     /locus_tag="PLD15_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPB63081.1"
                     /translation="MTFEDLVNTSKTSKTPLHEVVCEWYMMNTGDDPQNALKTSLRLT
                     NEMFNSFEARKSNPSKSLTGWVGENDQKVRNSSPFLLSQIVYSGIEVALSMAEHNASM
                     GKIVACPTAGACGVVPGALISMHKNLGKSVEELSHSLLVAGAVGERIRRKASISGAVS
                     GCQAEIGTATAMASAAIVYAVSPENYEALVSAPALALKSLLGLVCDPVGGFVEIPCVK
                     RNAFGVSTAFTAAELALAGIESVIPFEEVAESLGRVGRRLPEELKETGKGGIAVTQTA
                     RDMLAKFLGVDRRR"
     gene            complement(3842..4507)
                     /locus_tag="PLD15_03925"
     CDS             complement(3842..4507)
                     /locus_tag="PLD15_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HPB63082.1"
                     /translation="MLLDIIGPVIVGPSSSHTAGMARLGRAFRSLLNGRPDDVKFLLN
                     SPLFATHRGHGTDRALVGGVLGMKESDPSLKQALEMAVQSGMSFSFEEVDMPEAHPNT
                     VKITGQNSDRFLSITGASIGGGEIRITSIDDFETGVNGKYPSLILINADVPGALAQIV
                     RLVSASDINISNLLLSRVDPYRREALCVIELDDEPDQDLIVEIKKLKVVRRVSYLEKL
                     DVV"
     gene            complement(4569..5852)
                     /gene="eno"
                     /locus_tag="PLD15_03930"
     CDS             complement(4569..5852)
                     /gene="eno"
                     /locus_tag="PLD15_03930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB63083.1"
                     /translation="MYAQIVDLVAREVLDSRGTPTVEAEVWLDDGGHGRAIVPSGAST
                     GKFEALELRDGDKKRFLGKGTLKAVENINEVIAPEIVGLNAFDQTAIDDSLLKLDGTK
                     NKDKLGANAILAVSMAVARAAADSIDTLLYKYLGGTNAKVLPIPFMNIVNGGKHADNN
                     LDIQEFMIVPAGFKYFRDALRAGVEVFHHLKKLLKDGGHVTAVGDEGGFAPSFESNEE
                     AIKYIINAVESAGYKPGEEIFIALDCAASEFFNEEKGLYTIDGKELTPDQLIDYYVDL
                     TERYPIISIEDPFNEEDWGAFSKLTERVGGKVQIVGDDLYVTNIERLRKGVEERASNS
                     ILIKLNQIGSVTETLDTIEYAQKHGMTCVISHRSGETEDTFIAHLAVATNAGMIKTGS
                     ASRTDRIAKYNELLRIEEELGEQAEYRGLSSFYNL"
     gene            complement(5866..6567)
                     /locus_tag="PLD15_03935"
     CDS             complement(5866..6567)
                     /locus_tag="PLD15_03935"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HPB63084.1"
                     /translation="MYKRVLLKLSGEVLSGEGGRGFDEASVNYLLEEILPVIRTGTQL
                     AIVIGAGNLVRGRELRNLRNSRADELGMLGTVMNAVYLKEILSEAGVKAVAISSIVKL
                     PSLDDHKYDRIEKSLNSGEVVIFGGGTYLPFFTTDTAAAVRAVEIGSDVIIKGTKVDG
                     VYDKDPKKNDNAAKIDRITFASAIENNIEVMDREAFSICQRYGVPVIVIDFFKKRNLL
                     NAIMGEKIGTLVIPD"
     gene            complement(6619..7224)
                     /gene="tsf"
                     /locus_tag="PLD15_03940"
     CDS             complement(6619..7224)
                     /gene="tsf"
                     /locus_tag="PLD15_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPB63085.1"
                     /translation="MAEITSDMVKQLRDATQAGMMDCKKALVETKGDFELAKDYLRKK
                     GILKADKVSGRETGEGIIYSYIHHNNKLGVLLELDCVTDFVARTDEFKELAHKISLQL
                     AAMGARFVSRDLVPQEKIEKEKEIYAEQVKESGKPAHIIEKIVESKLESFYKENCLLE
                     QEYVFESGKTINDLIVELISKTGENIKVKKFVRWQVGESDS"
     gene            complement(7304..7822)
                     /gene="coaD"
                     /locus_tag="PLD15_03945"
     CDS             complement(7304..7822)
                     /gene="coaD"
                     /locus_tag="PLD15_03945"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPB63086.1"
                     /translation="MPLKKGRTGGAPIFMKAVYPGSFDPITYGHIDLVERSSKIFDEV
                     IVLVMENVNKKHFFAHEERMRMVRLAVSHIHNVTITTHSGLLVDFARENDVKVVIRGL
                     RAVSDFEMELQMAHANKSMLADFETLFLMTETSNSFISSSMVREIASFGGDISKWVPP
                     CVKGEFKRKLGK"
     gene            complement(7850..8311)
                     /locus_tag="PLD15_03950"
     CDS             complement(7850..8311)
                     /locus_tag="PLD15_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPB63087.1"
                     /translation="MGFTLKSSYALRALYKMARSARNGKDKMSLVEIVSDNEIPRDFL
                     EKIFGELRQAEIIKAVRGRYGGYSLAKPADKILIRDIILKLDRPMNSYACLQKDGKCV
                     EDPDCTVKYVWFRLYNAMMREIGSMTLEDLLEYGDRILNNPPDKLGIEEHT"
     gene            complement(8315..9226)
                     /gene="ftsY"
                     /locus_tag="PLD15_03955"
     CDS             complement(8315..9226)
                     /gene="ftsY"
                     /locus_tag="PLD15_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPB63088.1"
                     /translation="MGIFSRLKEGLKKTRDGFFKNVKKLLSFDRLDEETLEEIEELLI
                     LSDMGSETSQDVIKALRERMHKGKNPIDVLKDVLIEILDIPYEQKTAKPHVISVVGVN
                     GTGKTTTIGKLSQLYSQQGKKVVLAACDTFRAAAVEQLRIWSDRSGVDFISQGQGADS
                     AAVGFDAINHAISKGKDVVIIDTAGRLHTRSNLMEELKKVHRVIGKAAQGAPHEVLLV
                     IDATTGQNGLIQAAKFKEAVNVTGIVLTKLDGTAKGGIIFAIVKELGIPVKYIGVGEK
                     IDDLKPFKPSEFVEALLSSDEESSEEV"
     gene            complement(9226..10974)
                     /locus_tag="PLD15_03960"
     CDS             complement(9226..10974)
                     /locus_tag="PLD15_03960"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPB63089.1"
                     /translation="MRFSQLYAPTLKEAPSDADLVSIKLLIRGGFVRKVVAGVYTYLP
                     LGLKVLKKIEEIVRQEMNAIGSQEILMPIIQPAELWHETGRWDDYGPEMMKLQDRHER
                     FFTLGPTHEEIITHILRNELKSYKQLPVSLYQIAMKYRDEIRPRFGLMRSREFLMKDA
                     YSFHDSWESLDEAYKDFYSAYGKILERLGLDYLVVEADSGAIGGNESHEFNVLARYGE
                     STLLYCDCGYAASDEKAEYLFESVLQDREPAELELISTPQSRTVEEVTAFLGISSRDI
                     IKSLIYSGRNGYVMVLIRGDEELNESKLKASMNDQTLTMATPQQIFELLRVPVGFVGP
                     VGLPGGIEIVADLSIKGLKNAVGGALKEGFHYRGILPERDFKIGKWLDLKMVKTGDAC
                     PKCGKPLKSAKGIEVGHIFKLGTKYSDKMEGAVTDAQGNAVPYVMGCYGWGVSRTIGA
                     IVEQLHDKDGILWPLEAAPFDIIVTAVNTKDEEIMKRSVEIYGLLKKKGYDVLLDDRE
                     TSPGMKFKDADLIGIPVRVTLGKKLAEGVVEVKSRTEEKPVMVSVKDGYGPIVEEVER
                     LFASYDPESVLKPEDE"
     gene            11096..>11890
                     /locus_tag="PLD15_03965"
     CDS             11096..>11890
                     /locus_tag="PLD15_03965"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPB63090.1"
                     /translation="MSISDLQDELKTYVENIKKAAHFIQKEVPTKPKVAIILGSGLGD
                     ISRSLENSISIPYEDLPGFPVSTAPGHKGELTFGNVLHHEVMLMNGRFHYYEGYCMRT
                     ITFPIRVMQELGVEVLIITNASGGLNPDFEVGRVMLVRDIINFMGDNPLIGPNVDEWG
                     PRFPDMSEPFDLELLEKAKLASKELGIGVYEGVYIGVAGPNFETPAELRMMRRFGADS
                     VGMSTVPEVIVARHAGIKVIGFAAISDRAVPDDLKPITAEEVLEMAK"
BASE COUNT         2669 a         3240 c         2612 g         3369 t
ORIGIN      
        1 ggagaagata taagatcgaa aagaatgtcc agcgcctcgt gcgaataggt gtccggaact
       61 ttcgcgtagt aaaccgtaga tatcctgccg gtataggcgt tgagacttcc acctattctc
      121 tcgatcggtt cctttatctc gaatgcgttt ctgtctttgg ttcccttgaa tagagtgtgt
      181 tctatgaaat gagaaactcc gctgatggga tcatcctcat cagcggatcc tacttttacc
      241 gcaaaagcca tcgaaaccgt cctggtttct tcttttcttt cgccgacaag agtagcgtta
      301 tttgaaagtt ccttataaag aatgctcaat gtcgcttacc tactttctgt ttcgctcgtt
      361 tctgtttcta ccttcgtttc ttggataggg tttcctctct cttctctgtt cctcggtcac
      421 ttcgacgcct tccagtttca ggcttatctt acccatctga tcgatacccg agacttctac
      481 ctttattatg tctcctaatt tgtgcgcttt aaggaattct ttcgaattct cacccatctt
      541 cgacacgtgc aggagtcccg tctttcccgg cgcgagttcc acaaagaatc cataagcctc
      601 ttcgcgcgtg atctttcctt cgaatatctg gccgggtttc gcctcggata ttatagcctc
      661 gatgactttt tcgagtctct cgagtttagc gtggtcgttc cccacgatct tcgcgagtga
      721 cttctcgtcg tctatgaaga ttgtcgagtc gtaatcgctc gaaagcttct tgataacctt
      781 tccaccgggg ccaattatct cgcctatctt ttcataagga agcgcaatcg tcttgatgat
      841 cggtgcataa tcggacacag tcgttctagg agtatctacc gcccggtaca taaggtcaag
      901 gattttcagt ctcgcaactt tcgcctgttc aagggcctgc gtcattatat ctccggaaac
      961 accagatact tttacatcca tctgaaaggc cgtaataccg tcgcgggtac cggtgacttt
     1021 gaaatccatg tcacccatat gatcttcgtt tccaagtata tcggtcagga ccaccgtttt
     1081 atctggctcc tgaatcatac ccattgcaac cccggcaacg tgtttcttca tcggtactcc
     1141 tgcagccatg agagccagag atcccgaaca gactgttgcc atcgaggatg atccgttgga
     1201 ttcgaggatc tccgagacga ctctgatcgt gtaaggaaag tcttcgtcct tggggacaat
     1261 gttcttgaga gatctttcgg ctagatgacc gtgacctatc tctctccttc cagggcctct
     1321 caatcttttc acttctccgg tactgaaggg agggaagttg tagtgcagca tgaaggattt
     1381 cgagccctct tcgaacatcg tatccactat ctgttcatcc atggtggctc ccagggttac
     1441 tatgccaagg ctctgtgtct ctcctcttgt gaagagggcc gaaccgtggg ctcttgggag
     1501 gagatccagc tctatcgtga tcggtctgac ctcgtcgtgc tttctcccgt ccattctcac
     1561 atcgtcctga acgatccttc ttctcatcac ttctttctcc acgtcgtgat aggaatcctt
     1621 caggtaaccg atgtttccca ttatctcttc ttctgaccag cgatcgttaa ccttctggac
     1681 gaaggcctcg atgagttgat ccctgtattc tttcaacgct ttatctttgg catgtttccc
     1741 gggtgtaagc atcagcccgg ccagtttatc tctatctacg aattccccga aggcttcgac
     1801 gaaaccttcc ggagcggtcg ggatttccag ctcccatttt ttgatttcga attcagccag
     1861 tatctcctcc tggaagacca ccagagactt tatcgcctca tgcgcggctt ccagcgcgcc
     1921 gaccattatc ttttccgata cttctttcgc ctcgccttcg accattgtca cggcatgttt
     1981 ggtgcccgcg accacgatgt ccagttccga tcgcttcagt tcttcggccg atgggaatat
     2041 gacgtacttc ccatcgatat atccaacctg aactccggca actactcctt cgaagggtat
     2101 ctccgaaagg tttagcgcaa gcgatgctcc cattattccc cagatatccg gtggattgtc
     2161 gggatcggcc gaaagcacgg tgactataac ctgtatctcg tttctcatac cgtcttgaaa
     2221 cagcggtctg ataggtctgt ctattattcg cgcagataga accgcgttgt cgctgggtct
     2281 gttctccctt ttcagaaacc ctcccggaat ctttccagcc gcgtaaaact tttcctgata
     2341 ctctacagtc aggggcaaaa aatccgttcc gggcatcgct tcttcgttcc catcaacggt
     2401 ggtgagcagt accgaatcgg cgtatctgag caaaattgcc ccgtgggcct gttttgccat
     2461 cttgccgttt tcgatcacca gcttctgacc aaaaaactct ctctcccatt gtttgtagct
     2521 cactaacaac acctcttttt cgattctcaa atactcaatt ataacagttg cataaaattc
     2581 acgcaaaaaa ggagcggtta cccgctccct gttgagttcg ttgtattctc aacccctcaa
     2641 tccaagcttg ttgatcagct ctagatatac ttcgggtttc ttggccttta tatatctgag
     2701 catctttctt cttcttccga cgagtttcag cagtcccctt ctcgtatgga agtccttggg
     2761 gtgcttcttt agatgctcgg tgagatgtct tatcctggcc gtcaggatcg ctatctgaac
     2821 ttctgtggaa ccggagtcgt tgtcgtggat ttgaaattcc ttgacaagtt cctgcttgtt
     2881 gatttccatg tctctcctcc ttatgagtcc tctatccagg aaagggctat acctcgaacc
     2941 gggaataggg caaggcaatt ctaaaacgat tatcttcttc tgtcaacacc caggaacttt
     3001 gccagcatgt cccgtgccgt ttgagtgaca gctataccgc ctttgccggt ctctttgagc
     3061 tcttcgggaa gtctccttcc caccctgccg agagattcgg ccacttcttc aaacggtata
     3121 accgattcta tgccggccaa agcgagctca gccgccgtga aggccgtaga tactccgaaa
     3181 gcgtttctct tgacgcaggg aatttctaca aagccgccga ccggatcgca caccagtcca
     3241 agcagcgatt tcaacgccag agccggtgcg gaaacaaggg cttcgtaatt ttccgggctc
     3301 accgcgtaca ctattgcagc cgacgccatg gcagtcgccg ttccgatttc ggcctgacaa
     3361 cccgacacag caccggagat cgatgccttt ctccttattc tctctccaac cgcgccggcc
     3421 accagcaaag agtgagataa ctcctccaca ctctttccaa ggtttttgtg catggagatc
     3481 aacgctccgg gaacgactcc acacgcacct gccgtcgggc aggccacgat ctttcccatg
     3541 ctcgcattgt gctcggccat agacagggcg acctctatac cagagtaaac gatctggctt
     3601 agcagaaaag gactggagtt cctgactttc tggtcgtttt ctccgaccca tcctgtcaga
     3661 gatttagaag gattagattt tctggcctcg aaggagttga acatctcatt tgtcagtctt
     3721 aacgatgtct tcaaggcgtt ttggggatca tcgccggtgt tcatcatgta ccattcgcaa
     3781 accacttcgt gaagtggagt tttcgaggtt ttagatgtat tcacaaggtc ttcgaaggtc
     3841 atcatactac atccagcttc tcaaggtaag agactcttct cactaccttc agttttttta
     3901 tttctacaat caggtcttga tcgggctcat cgtcgagctc aataacacag agagcctcgc
     3961 gtctgtacgg atccaccctg gacagcaaca gattgcttat gttaatatcg ctcgccgaca
     4021 ccagtcgcac tatctgagcc agagcgccgg gaacatcggc gtttatgagt ataagcgagg
     4081 gatactttcc gttcacgccg gtctcaaagt catctatcga tgttatcctg atctctccac
     4141 ctccaatgga ggctcccgtg atactgagaa atctatcgga gttctgtccg gttattttta
     4201 ccgtgttcgg gtgagcttca ggcatatcga cctcctcgaa gctaaaagac ataccgctct
     4261 gcacggccat ctccagagcc tgcttgagag agggatcgga ttctttcatg ccaagcactc
     4321 ctccaaccag agccctgtcc gttccgtggc ctctgtgggt ggcgaagagg ggggagttga
     4381 gaagaaattt gacatcgtcc ggccttccat tcaaaagcga cctgaaggct cttcccagcc
     4441 tcgccatacc ggccgtgtgg gaactagaag gcccaactat caccggtcct attatatcca
     4501 gcaacatctc tcttacctcc gtgcaaaagg cgcgggaatc ggagaatccc cgcgccccga
     4561 aaaaattctc agagattgta gaaagaagaa agtcctctgt actctgcctg ttcgccaagc
     4621 tcttcttcta ttctcagaag ttcgttgtac ttggcgatcc tatcggttct ggacgccgag
     4681 ccagttttga tcatccccgc gtttgtagcc acggcgaggt gggcaatgaa cgtatcttca
     4741 gtttcaccgg atctgtggga aatcacacag gtcataccgt gcttctgggc gtactctata
     4801 gtgtcgagag tctcggtgac cgaaccgatc tggttcagtt ttataagaat cgagttggag
     4861 gccctctctt caactccctt tctaagtctc tcgatgttcg tcacgtaaag atcgtcgccg
     4921 acgatctgga cttttccgcc gaccctttcg gttagcttcg agaaagctcc ccaatcctct
     4981 tcgttgaagg gatcttctat ggagattatc gggtatctct cggtcagatc tacgtagtaa
     5041 tctataagct ggtcgggagt gagttccttg ccatctatgg tgtacaaacc cttctcttca
     5101 ttgaaaaact cgctggccgc acagtctaga gctatgaaga tctcttctcc tggtttgtag
     5161 ccggccgatt caacggcgtt tattatatac tttatggctt cttcgttgct ctcgaaactg
     5221 ggagcgaaac cgccctcgtc gccgaccgcg gtcacgtgtc caccgtcttt caggagtttt
     5281 ttaaggtgat ggaagacctc gactcctgcc ctcagagcat ctctgaaata cttgaatccg
     5341 gccggcacta tcatgaactc ctggatatcc aggttgttgt cggcatgttt tccaccgttc
     5401 actatgttca tgaagggaat cgggaggacc tttgcgttgg ttcctccaag atacttgtag
     5461 agaagggtat ctatgctgtc ggccgcggct ctggcaactg ccatcgatac ggcgagtatc
     5521 gcgttcgcac cgagtttgtc cttgtttttt gtaccgtcaa gcttcaacag cgaatcgtcg
     5581 atggcagtct gatcgaaagc gttcagacct actatttcgg gagcgatcac ttcatttatg
     5641 ttctctacgg ctttcaacgt gccttttcca agaaatcttt tcttatctcc gtcacgaagc
     5701 tcgagcgcct cgaacttacc cgtagaagcc cccgaaggta ctatggccct tccatgaccg
     5761 ccatcgtcca gccagacttc ggcctcgacc gtaggtgttc ccctcgaatc aagaacctca
     5821 cgagcaacca ggtcaactat ctgtgcgtac atttcatcac ctccatcaat cgggaattac
     5881 aagcgtgcct atcttctcgc ccattatagc attcaaaaga tttctcttct tgaagaaatc
     5941 tataactatt accggtacac cgtatctctg acagatggaa aaggcttcgc ggtccataac
     6001 ttctatatta ttttcaatag cgctggcgaa ggttatcctg tctatctttg cggcattatc
     6061 gtttttcttc gggtctttat cgtaaacgcc atcgactttg gtgcctttta tgataacgtc
     6121 cgaacctatt tcgaccgctc tgacggcggc cgccgtgtca gttgtgaaaa aggggagata
     6181 agtacctccg ccgaatataa caacctctcc gctattcagc gatttctcga tgcgatcgta
     6241 cttgtgatcg tctagagaag gcaacttcac aatcgaggag attgcgacgg ccttcacccc
     6301 tgcctcgctg agaatttcct ttagataaac tgcattcata acggtcccga gcattccgag
     6361 ctcgtcggcc cttgaatttc tcaaattcct cagttctctg cctctcacaa ggttcccggc
     6421 acctataact atcgccagct gtgtgccagt tcgaattacc ggaagaatct cttcgagtag
     6481 atagtttacg gacgcttcgt cgaatcccct tccgccttcg ccgctcaaaa cttccccgct
     6541 gagttttaga agaactcttt tatacatacc cacctccgga gattataaaa aaaggtgagc
     6601 accgctcacc ttagaaactc aactgtcgga ttcgccaacc tgccagcgaa cgaatttctt
     6661 cacttttatg ttttcgcccg ttttggaaat caactcgacg atcagatcat ttatagtttt
     6721 gccgctctcg aagacatatt cctgctcgag cagacagttt tccttgtaga agctctcgag
     6781 cttgctctcg actatcttct caattatgtg ggcaggtttt ccgctttcct taacctgttc
     6841 ggcgtagatc tccttttcct tctctatctt ttcctgaggg acgagatctc tggagacaaa
     6901 ccgtgctccc atcgctgcaa gttgcagaga gattttgtga gcaagttcct tgaattcgtc
     6961 ggttctagcg acgaaatcag taacacagtc aagttcgaga agcacgccga gcttgttgtt
     7021 gtgatgaatg taagagtata ttattccctc tccggtttct ctaccggaga ccttgtcggc
     7081 tttaagaatt ccctttttgc gaaggtaatc ctttgcgagt tcgaaatcgc ccttggtttc
     7141 tacgagagcc ttcttacaat ccatcatacc ggcctgagtt gcatcgcgga gttgctttac
     7201 catatcactg gtaatttcag ccattattgt gtgtcctcct tcattaaaat tccatcgaag
     7261 attataactc agttgaacgc agtttatttt ctgcgatgta aagtcattta cccaattttc
     7321 ttttgaactc acctttgacg cacggtggga cccatttaga aatgtccccg ccgaaagagg
     7381 cgatctccct gaccatggac gaagagatga aggagttgga ggtctccgtc ataagaaaca
     7441 acgtctcgaa atcagctaac atggacttgt ttgcatgggc catctgaagc tccatctcga
     7501 agtccgagac cgctctgagc cctcgaatca caacctttac atcgttctct ctggcgaagt
     7561 ccacgagaag accgctgtga gtggttatgg tcacgttgtg tatgtgagaa accgcgagtc
     7621 tgaccatccg cattctctcc tcgtgtgcga aaaaatgttt cttgtttacg ttttccataa
     7681 ccaacacaat cacttcatcg aagatcttgc tcgacctctc gaccaggtcg atgtgcccgt
     7741 aagttattgg atcaaaagaa ccaggataga cggccttcat gaaaatcggc gctccgcccg
     7801 ttctcccttt cttcaagggc aaagcgtcat cctccttccc gtaagatgac taagtgtgtt
     7861 cctcgatccc gagtttgtcc ggcgggttgt tcaatatcct gtccccgtat tcaaggagat
     7921 cttcaagtgt cattgaacca atctctcgca tcatggcgtt atataacctg aaccagacgt
     7981 atttcaccgt acagtcagga tcctcgacac atttgccgtc cttctgaagg caggcgtagg
     8041 agttcatggg tcgatcgagt ttcaagatta tatccctgat caatatcttg tccgccggtt
     8101 tcgccagaga ataaccacca tacctgcccc tgacggcttt gattatttcc gcctgcctaa
     8161 gctccccaaa gatcttttcg aggaaatctc tcgggatctc gttatcgctc acgatctcaa
     8221 caagagacat tttatctttt ccgttacgtg ccgatctggc catcttgtaa agagctctca
     8281 gcgcataact acttttgaga gtgaaaccca tactctaaac ctcctcagaa ctctcctcat
     8341 cgcttgaaag gagagcttcc acgaactccg acggcttgaa gggctttagg tcgtctatct
     8401 tctcgccaac acctatatat ttcaccggta tgccaagctc cttcactatg gcaaagatta
     8461 tgccaccttt cgccgtaccg tccagtttcg tgagcacgat acccgttaca ttcacggctt
     8521 ccttgaactt cgcggcctga ataagaccgt tttgacctgt cgtagcgtct atgaccagta
     8581 gtacttcgtg cggagctccc tgggctgcct tcccgatcac cctgtgtacc ttcttcagct
     8641 cttccatcag gttgcttctg gtgtgaagtc tcccggcagt atctatgata acaacatctt
     8701 tccctttgct tatggcgtgg tttatagcgt cgaagcctac cgccgcggaa tcagcgccct
     8761 gaccctggct tatgaaatcg actcccgacc tgtcactcca tattcgaagc tgttcgaccg
     8821 ccgcggccct gaaagtgtcg caggccgcaa gtaccacttt ctttccctgt tgagaataga
     8881 gctgcgacaa cttgccgatc gtggttgtct tgcccgttcc gttcacgccc accaccgata
     8941 tgacatgggg cttcgcggtt ttctgttcat aagggatatc gagaatctct ataagcacgt
     9001 cttttagaac atctatcgga ttttttcctt tgtgcattct ctctctcaga gcctttatta
     9061 catcttgaga agtttctgag cccatatcgg agagtataag caactcctcg atctcttcga
     9121 gcgtttcctc gtctagcctg tcgaatgaaa gaagtttttt gacgttcttg aagaaaccgt
     9181 ctcgagtctt cttcagccct tccttgagtc tggaaaagat tcccattact catcctccgg
     9241 tttcaatacg ctctcgggat catagctcgc aaaaagcctt tcgacctctt caacgatcgg
     9301 accgtagcca tcctttacgc tcaccatgac aggcttttct tcagtcctgg acttgacttc
     9361 tactacgcct tcggcaagtt ttttgcccag ggttacacgc accggtattc caatgaggtc
     9421 tgcgtctttg aatttcatgc ccggtgaggt ctcccggtcg tcgagaagca catcgtaacc
     9481 ctttttctta agtaatccat atatttcgac ggatcttttc attatctcct cgtccttggt
     9541 gttgaccgcc gtaacgatga tatcgaaagg cgccgcctcc agaggccaga gtataccgtc
     9601 tttatcgtgc aactgctcaa caatcgcccc tatggttctc gacacccccc agccgtaaca
     9661 gcccataaca tagggaactg catttccctg agcgtcggtg accgctccct ccatcttgtc
     9721 cgagtacttc gttcccagtt tgaatatgtg accgacttcg ataccttttg cagatttcaa
     9781 aggttttccg cacttcgggc aggcatcccc ggtcttcacc atcttgaggt ccagccattt
     9841 cccgatcttg aaatcgcgtt ccggcagaat gccacgatag tgaaaacctt ctttcaaagc
     9901 tccacccact gcgtttttca aaccttttat cgaaagatca gccacgattt ctatcccgcc
     9961 gggcagaccg accggtccga caaagccgac aggaactctc agaagttcaa aaatctgctg
    10021 gggggtcgcc atagtgagtg tctgatcgtt catcgaggcc ttcagcttcg attcgttgag
    10081 ttcttcgtct ccccttatca aaaccatcac gtacccgttt cttccactgt atataagaga
    10141 ctttataata tccctggaac tgatacccag aaaggccgtt acttcctcga ccgttcgaga
    10201 ttgaggtgta gaaatcagtt cgagttccgc aggttcccga tcctgcaaga ctgattcgaa
    10261 gagatactca gccttttcgt ctgatgcggc ataaccgcaa tcgcagtaca aaagcgtgga
    10321 ttcaccgtac ctggccagaa cgttgaattc gtgagattcg ttaccgccga tagctccgga
    10381 atcggcttcc actactagat aatccagtcc gagcctttcg aggatcttac cgtaggcaga
    10441 gtagaagtct ttgtaagcct cgtcgagaga ttcccaacta tcatgaaaac tgtaggcatc
    10501 cttcatgaga aactcccgcg acctcataag accgaacctc ggccttatct cgtcccggta
    10561 tttcatggct atctggtaga gagaaaccgg caattgcttg tatgatttga gttcgttgcg
    10621 gagaatgtgt gtgattatct cttcgtgtgt cgggccgagt gtgaagaacc tctcgtgacg
    10681 atcctgcaac ttcatcatct ccgggccgta gtcgtcccag cgtcccgtct cgtgccagag
    10741 ctcggcaggc tgtattatgg gcatcaatat ctcctgcgaa ccgatcgcgt tcatttcctg
    10801 gcgaacaatc tcttcaatct tcttcaagac cttcaagccg agcgggagat atgtatagac
    10861 tcccgcgacc acctttctga cgaagccgcc tcttatcaaa agtttgatac ttacaagatc
    10921 tgcatcggat ggagcttcct taagggtagg agcgtaaagc tgtgagaacc tcatcgtaac
    10981 tctcctctca acaaaaaaac taaatgggac taattcggtg tgattatagc acagttccag
    11041 cacgctgtcc gtcgttttgc tataatagaa tatcgatttt agagggaggg ccggcatgag
    11101 tatttctgat cttcaagacg agttgaaaac ttacgtggaa aacatcaaaa aagctgccca
    11161 tttcattcag aaagaggtac ccactaaacc gaaggtcgcc ataatactgg gcagtggact
    11221 tggagacatc tccagaagtc tcgaaaactc catctccata ccctacgagg atttgccggg
    11281 ttttccagtt tctacggctc cgggtcataa aggtgaactc acctttggaa acgtactcca
    11341 ccacgaagtg atgcttatga atggacgttt tcattactat gaaggatact gcatgagaac
    11401 aataaccttt cccatacgcg taatgcagga actcggtgtg gaggttctca tcatcaccaa
    11461 cgcttctgga ggattgaacc ccgactttga agtggggagg gtgatgctcg tccgcgacat
    11521 aataaacttc atgggcgaca atccgctcat aggacctaac gtagatgaat ggggaccgag
    11581 gtttcctgat atgagcgaac ctttcgatct ggaactcctc gagaaggcaa aactcgcttc
    11641 gaaagaactc ggtatcggtg tttacgaagg ggtttatatc ggcgtggcag gaccgaattt
    11701 tgaaaccccg gccgagctga gaatgatgag gagattcggc gccgattcag tcggaatgtc
    11761 aacggttccc gaggttatcg tggcccgtca cgccggtatc aaagtgatag gtttcgcagc
    11821 catatcagat cgcgccgtac ccgacgattt gaagccaatt acggccgaag aggttcttga
    11881 gatggcgaaa
//
LOCUS       DAOOLY010000049        11485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6305_length_11485_cov_4.981540, whole genome shotgun sequence.
ACCESSION   DAOOLY010000049 DAOOLY010000000
VERSION     DAOOLY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_11485_cov_4.981540"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLD15_03970"
     CDS             complement(<1..786)
                     /locus_tag="PLD15_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPB63091.1"
                     /translation="MKKLSILALLVVTFATLALGVEYSDLSENHWAYDSVIKTTAGGL
                     FIGFPDGTFRGNENVTRYQLAMTLARFMDYSDAGDAKLQEVVFALTKKVAGLSLEISD
                     NKKNLVDFMAELRALEATVASLKDSGIVGNFVSSKTFDDTVAYLYREIDRLSGEAASA
                     NEGVAGLRADLEALLGVRDDIAGLDAKLKAVGGYASEIEALKNRATDLEVTVKLLGQA
                     LANQKVEENEQKGEIADLDSRIGVVENVANSLTSDLFALRKAMV"
     gene            complement(875..2071)
                     /gene="tyrS"
                     /locus_tag="PLD15_03975"
     CDS             complement(875..2071)
                     /gene="tyrS"
                     /locus_tag="PLD15_03975"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB63092.1"
                     /translation="MQTPEDQFKELSKNHVSLISEKELLEKFSKNRPLRVKLGVDPSR
                     PDLHLGHAVVLRKLRQFQQFGHQVVLIIGDFTARIGDPSGRSKTRPMISKDEAKINAI
                     SYSKQAFKILDKDLTEICFNSQWLDALNFEDVIRLSAKYTVARMLERHDFAKRYSDNE
                     PIAIAEFLYPLAQAYDSVFVKADIEIGGDDQFFNLVVGRKIQEEYGLEPQAVLTVPLI
                     EGTDGNLKMSKSYDNYIAFEDTPRDMFGKLMSIPDTLIMKYMRLLTDKNDEELRDAEE
                     CLKSRTVNPRDIKMELARDITAQFYDEETSRAALEEFIKIFRHKEVPEEMPEIRLDTG
                     SVSVVDLLVKYANISSRSEARRLLEQGGIKLNEKVIEDVYAVLDPSDGDVLRIGKKRF
                     YRLVKC"
     gene            complement(2082..2333)
                     /gene="secG"
                     /locus_tag="PLD15_03980"
     CDS             complement(2082..2333)
                     /gene="secG"
                     /locus_tag="PLD15_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB63093.1"
                     /translation="MVVLAYVLISLHVAISVALGVAVMMQMSKSAELGGAFGGGASHT
                     MFGRKKGLDTMGKITLVLAVGFMVNSVLIAFVVSRAFPG"
     gene            2472..4121
                     /locus_tag="PLD15_03985"
     CDS             2472..4121
                     /locus_tag="PLD15_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB63094.1"
                     /translation="MAKLNNLPVRLPILAEDLPLAIDRDKIELEVVLRGLKAHYINEP
                     DDYYGSYLQFYCYEAFKKAIGDNELERSLGFLEDAAKISRDYRYHFYKGLYYRQTGNG
                     ELCEIELKRSVEMNPDFYVGFYEIGKLLRSNGDYEEAANYFLQSIEKSEGKFALPFLG
                     IVDTYISSGEYGQAMEIMNSIPRSFSLYSEILLRKGVVLNELQRFGEAEKVFDECIAI
                     ENRWQCFYNRSFSRSRQGKLWASLEDLRRAHEISGEVDVLYEEAIAEKNLGLLDEALE
                     HCEEYYFHTGDEKALFLLVRILDMLGRYEKALKYLEKGDYSELKNTVLFHRGLAKGKA
                     IAGLVFDNPITQAAYLSTLADIDKSTISREAGGLVTQGRLDIGSLINHLMKFDIAGMR
                     ERTTSFQMGMIPETSRIASLGEAGVYVSILKTLLDLPSRRDLLSYSMAFLISGLGTTT
                     AVFRILLHVLRWRRSGMAFNLELFLDEYLEEIRDLDFQLGLRITKLLEEGPIDIDTID
                     ESGIEDIEGFVLSMLSALEKGTIDQLDTNDENLKEYLKLLV"
     gene            4132..4389
                     /locus_tag="PLD15_03990"
     CDS             4132..4389
                     /locus_tag="PLD15_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPB63095.1"
                     /translation="MDRIEELLGRFMETIQSRNIDQIDEATLNRILHEEFDVSERLEL
                     EEALGSLLGEVSKMAEDPKEYFFRNDEEKLMEYLKEKEEKD"
     gene            complement(4472..4888)
                     /locus_tag="PLD15_03995"
     CDS             complement(4472..4888)
                     /locus_tag="PLD15_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPB63096.1"
                     /translation="MSFDDIYSDFIMHHYRNSGHRGIDKESDLMEKGANLSCGDEITL
                     FVRMKEGKITSLAFDGHGCAISQASASVMADLLEGKTLEEARKTLSNFSKMLRGEIFD
                     ENLLGDAVLFERLREYPMRIKCVSLAWKTAERALMK"
     gene            complement(4875..6107)
                     /locus_tag="PLD15_04000"
     CDS             complement(4875..6107)
                     /locus_tag="PLD15_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPB63097.1"
                     /translation="MFSPEDLNGYFKGLKGDFPIFSHDPSLVYLDSAATTQKPSAVLA
                     AVRDFYERNNSNVHRGVYPLAEAATELYESSRRRLSGFVGCWPGELVFTKGSTEGLNV
                     LASSLSISGDYDTFIVPVFEHHSNFVPWQYHARRNGLRFIPLPVRQNSLDMSLVYETL
                     QQMDGRFVFSLAGLVNSTGYRPPFEEIGSLVHSAGGVMVLDSAQLIPHEKFSFGESNV
                     DFLVFSGHKMLAETGIGCLVGREKLLGELNPFVFGGQMIDMVGKEETTFAEGVAKFEG
                     GTQNISGAISMMAAVEYLENAGMERVSAHVSNLVAMAREKIEAIGGYRIHSPESSRAV
                     ITFTHSRVHSHDIAEFLGRKMNVALRSGHHCSQLQMKELGVVSACRVSFYLYNTFEDV
                     DRLADSLEEAGRWFDEFR"
     gene            complement(6094..7281)
                     /locus_tag="PLD15_04005"
     CDS             complement(6094..7281)
                     /locus_tag="PLD15_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPB63098.1"
                     /translation="MEKTLNLEHNKYKMVSPLRRELEIPDYRDRDFLVSSEPLGNAMS
                     VFVSNRYSEYRKLGFPRWKRVDISKMVLPPVSPKNGKHYPDPYVKPIKWLTSEEVELL
                     DNLDFEGSDRKLLLLGDIFFDTGYFVSVPSGIHLKEPYIFNREEMNSSISSNLFVAGK
                     GSKMKIVLNSTGRGKWSLQNNRFLLKRESSIDLLVVNGLSKSDFSFLNNFYLLEAGAR
                     LNVYEVSYGGGVLASYHMGVAAGEESRTVFEPVFMAYGDSKIDLQYIARVKSARSQAI
                     IDGTGVVMDRGHSIFRGTVDLKKGSRGATGKEHSSVMVLSREARTDAIPSLLVDENEV
                     NASHAATVGSLDEEKIFYLMSRGLTREEALKFIIKGTFDPVLGKVVELFPDHSRGLDR
                     VFA"
     gene            complement(7290..8405)
                     /gene="sufB"
                     /locus_tag="PLD15_04010"
     CDS             complement(7290..8405)
                     /gene="sufB"
                     /locus_tag="PLD15_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPB63099.1"
                     /translation="MPKEIRETFERLGIPEAERNSLAGVGAQYDSEVVYQNIKKNLEE
                     LGIIFLDMETAVKEYPEYVEKYFMKAVPPSDHKYSALHGAVWSGGSFVYVPKNVSVPL
                     PLQAYFRMNMASSGQFEHTLIIADEGSNLQFIEGCSAPRFNELNLHVGMVEIFVLKGA
                     KVRYSTIQNWSKNTYNLNTKRAIVEEDGTMEWISGSLGSMKTMLYPATILKGRRARAE
                     HLGITYAGPGQHMDTGSKVIHLAPETSSVVDARSISTGGGWAFYRGALKISRQADNAK
                     SSVQCTALMMDNDSRSDTVPIIEVENDSANIGHEAKIGRISEEQIFYMMSRGLSEAQA
                     RSMIVRGFMEPVSREFPVEYAVELNRLIDLEIESSIG"
     gene            complement(8359..8682)
                     /locus_tag="PLD15_04015"
     CDS             complement(8359..8682)
                     /locus_tag="PLD15_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63100.1"
                     /translation="MDRDLFLDSSRFDFVSTTDYSYISEKGFDEELIRQISTKKDEPE
                     WMLRKRLESFEIFNRSLEPGFGVGTGDLDLQHSSLSQARFRAKPFLGGSAQGDKRNLR
                     KARYP"
     gene            complement(8685..9437)
                     /gene="sufC"
                     /locus_tag="PLD15_04020"
     CDS             complement(8685..9437)
                     /gene="sufC"
                     /locus_tag="PLD15_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPB63101.1"
                     /translation="MNLLDIKELRAALREDGKEILKGVNLKVRTGELHAIMGPNGSGK
                     STLANVVMGNPKYEITSGKIRFKNKTINDLSVDQRARLGIFMSFQYPQEISGIRLRSF
                     LISASHNLKNDEPILKKSRELERLADEIGIGREFIDRYLNLGFSGGEKKKTEIMQMHF
                     LKPDLAILDEIDSGLDVDAMRSIATSIRNFRSPENGVILITHYERLLEYIEPDYVHIY
                     VDGSIVMTGGPELARDIEERGYERNWKSYREA"
     gene            9573..10418
                     /locus_tag="PLD15_04025"
     CDS             9573..10418
                     /locus_tag="PLD15_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPB63102.1"
                     /translation="MFYVITIGVIMGLANLIPGVSGGTVVLLGGLYERFVGAVASLSE
                     FKFEKKQLMFLVQLFAGIIIGLLAFSGLIELSLTNVPSLMYGIFCGLVVGSIPVVLKN
                     IKKFTPASAICFAVGAVLVLTMAFSGARPGDDALLTHSLTAFLYDLLAGFVGAAAMVL
                     PGLSGAFVLLLMGEYSRAISALNDRDLLIILFIGLGVVGGIVFISKLLKSLMKKRQNE
                     TFSFLLGLMIGSIPDLLTRTGEATRLSLLIPGVALGVFLSYLLAVFERKNRKSPSSAV
                     DSNRQ"
     gene            complement(10494..10569)
                     /locus_tag="PLD15_04030"
     tRNA            complement(10494..10569)
                     /locus_tag="PLD15_04030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10534..10536),aa:Arg,seq:ccg)
     gene            complement(10548..11255)
                     /gene="ruvX"
                     /locus_tag="PLD15_04035"
     CDS             complement(10548..11255)
                     /gene="ruvX"
                     /locus_tag="PLD15_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPB63103.1"
                     /translation="MKILGIDFGTVRIGLAIQIEGVEIPLETIEHRDYRKSLKEIFSQ
                     REIDLTVIGLPISMSGRFNQLAMKAVSFAEKVKKIFSGRVILTDERLTTKSSERIAKN
                     SGLSFSTRRDMLSAMEILRNYSRGTTANWEVRDLFPVCRNFPPIDTGKKALLYAPLSS
                     KIEGLDSTKEMDVYVEDPQIYFSFLKKGFTPKNLLDDIDFSTYDIIVVTEGDKLPASH
                     IELADNCTVHMCSWLNG"
     gene            complement(11236..>11485)
                     /locus_tag="PLD15_04040"
     CDS             complement(11236..>11485)
                     /locus_tag="PLD15_04040"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPB63104.1"
                     /translation="TLLLMPQGTALPSLEALREAGITAKLFPTPPHSFPGCSISIAVS
                     SGNLECCLELLEQIGLEPLSIVHCSSNPVREFYEDTGN"
BASE COUNT         2770 a         2881 c         2744 g         3090 t
ORIGIN      
        1 aaccatagct ttcctcaaag cgaagagatc agaagtcagc gagtttgcaa cgttctcgac
       61 cacaccgatt cttgaatcga gatccgcaat ctcacctttc tgctcgtttt cctcaacctt
      121 ctggtttgca agagcctgac cgagcagttt cactgttact tcgaggtccg tagccctgtt
      181 cttgagagct tcgatttccg aggcgtaacc gccgaccgct tttagttttg catcgagacc
      241 ggcaatatcg tctctgactc ccagaagagc ctcaagatct gccctgagac cggctactcc
      301 ttcgttggca cttgcagctt cgccggaaag cctgtcgatt tctctgtaga gatatgcaac
      361 ggtatcgtcg aaagtcttgg aagagacgaa gttgcctacg ataccgctat ctttgagaga
      421 agcgacagta gcttcaagag ccctcagttc cgccatgaaa tcaactagat ttttcttgtt
      481 gtcagagatc tccagagaaa gtcctgcaac tttctttgtg agcgcaaaga caacttcctg
      541 gagtttggca tccccggcat cagaataatc cataaacctg gcaagggtca tggccagctg
      601 gtaacgtgta acattctcat tacctctgaa agtaccatcg gggaaaccaa tgaacaatcc
      661 accggctgtc gtctttatta cactgtcgta ggcccagtga ttctctgaca gatcgctgta
      721 ctcgacaccc agagcgaggg ttgcgaaagt aacaacgagt agagccagta tagaaagctt
      781 cttcatggat cctaccccct ttcaaaatgt tgtcattttt gcagcagact acgggtttat
      841 tatacagtaa aagcgtgccc agattcaaga gtcttcagca cttaacaagg cgatagaaac
      901 gcttttttcc tattctcaaa acatctccgt ccgaggggtc cagaacagcg taaacatctt
      961 cgatcacttt ctcgttcaat tttataccac cctgttccag gagccttctt gcttcgcttc
     1021 ttgaagaaat attagcatac ttcacgagaa gatcgacaac ggaaacactg ccggtatcga
     1081 gtcttatttc gggcatttct tccggcacct ctttgtgacg gaagatcttt ataaactcct
     1141 caagagcggc tctagaggtt tcctcatcat aaaactgggc ggttatatcc ctggccagtt
     1201 ccattttgat atctctgggg ttgacggttc ttgacttgag gcactcttct gcatccctga
     1261 gctcttcgtc gtttttgtcc gtgagaagcc tcatgtactt cattatcagg gtgtccggta
     1321 tagacatcag cttcccgaac atgtccctgg gcgtatcttc gaaggctatg tagttgtcat
     1381 agctcttgga catcttcaga tttccatcgg tgccttctat gaggggtaca gtcagtaccg
     1441 cctgcggttc cagaccgtac tcctcctgga tctttctgcc tacgaccaga ttgaaaaact
     1501 gatcgtctcc ccctatttca atgtcggcct tcacaaaaac ggagtcgtaa gcctgagcga
     1561 gagggtaaag gaactcggct atagctatcg gttcattgtc ggagtatctc ttcgcaaaat
     1621 cgtgtctttc gagcatcctc gccactgtgt acttcgccga taaccttatg acgtcttcga
     1681 aattgagagc atccagccat tgcgaattga agcatatctc cgtcaaatcc ttatcgagga
     1741 ttttgaaggc ctgcttgctg taggatatag cgttgatttt cgcttcatcc ttcgagatca
     1801 tcggacgcgt cttcgatcgg ccagaggggt caccgatcct ggcggtgaaa tcccctatta
     1861 tcaggacgac ttgatgaccg aactgctgaa actgccgcaa ttttctgaga accacggcat
     1921 gacccaaatg caaatctggc ctcgacggat ccacacccag cttcaccctt agaggtcgat
     1981 ttttggagaa tttttccagc agttctttct ccgaaatcag agaaacatgg ttttttgaaa
     2041 gttccttgaa ctggtcttca ggagtctgca atcaaaccac cttaaccagg aaaggctctg
     2101 gacacaacga aagcgatcag tacactgtta accatgaagc cgaccgcaag tacaagggta
     2161 atcttaccca tcgtgtcgag acccttcttt cttccaaaca tcgtatggga agctccacca
     2221 ccaaaggcac cgccgagctc ggcggacttc gacatctgca tcatgactgc aacgccaagc
     2281 gctacgctta tggctacgtg cagagatatc aaaacataag caagaactac catctaaata
     2341 cctcctgtat ctttccaacg ctcttctggt attatacaat atgtgcgact ttgtagcaaa
     2401 cgataataga gattgcgggt gagatattga aaggaaaaac tgtgatcatt tatcttccgt
     2461 tgaagccaga gatggcaaag cttaacaatc ttccggtcag gctccccata ctggccgaag
     2521 acctacctct ggcgatcgac agagacaaga tcgagctcga agtagttctg cgaggcctca
     2581 aagcccacta catcaacgaa ccggacgatt attacggctc atacctgcaa ttctactgct
     2641 atgaagcttt caagaaagct attggcgaca acgaactgga gagatcgctc ggctttctcg
     2701 aagatgcggc aaagatttca agagattaca ggtaccattt ttacaagggt ctttactaca
     2761 gacagacagg aaacggcgaa ctctgtgaaa tagaactcaa aagatctgta gagatgaatc
     2821 ccgatttcta cgttggcttt tacgagatag gaaaactgct ccggtcgaac ggcgactacg
     2881 aagaagcagc gaattatttt ctacaatcga tagaaaaatc cgagggaaaa tttgccctcc
     2941 cgtttctggg gatcgtggat acctacatct cttctggaga atacggccag gcgatggaaa
     3001 taatgaacag tattccccgc tccttttcgc tgtattccga gattctttta agaaagggag
     3061 tggttctcaa cgaactccag aggttcggag aggccgaaaa ggttttcgac gaatgtattg
     3121 ccatcgagaa tcgctggcaa tgtttctaca acaggtcttt ctcgagatcg cggcagggaa
     3181 agctttgggc tagccttgaa gatttacgaa gggctcacga aatttccggg gaggtcgatg
     3241 tcctctacga agaggcgata gccgaaaaga acctggggtt actcgacgag gcgctggagc
     3301 attgtgagga gtactacttc cataccggcg atgaaaaggc cctctttctg ctcgtgagaa
     3361 ttctcgatat gctcggtcgg tacgaaaagg ccctgaaata tcttgagaag ggcgattact
     3421 ctgaactgaa gaacactgta cttttccaca gaggccttgc aaaagggaaa gccattgccg
     3481 gactggtgtt cgacaacccg atcacgcagg ccgcctacct ttcaactctt gccgatatcg
     3541 acaaaagtac gatctccaga gaggcgggag gcttagttac gcagggaaga ctcgacattg
     3601 gatcactgat aaatcacctg atgaagttcg atatcgccgg aatgagagag agaacgacct
     3661 cttttcagat gggaatgatt cccgaaacct ccaggattgc atcgctggga gaggcaggcg
     3721 tttatgtttc aatccttaaa acgcttcttg acctcccctc ccggagagat ctgctctctt
     3781 attcgatggc tttcctgatt tctggacttg gtactacgac ggccgttttc agaatactgc
     3841 tgcacgtttt gcgatggagg cggtcgggaa tggctttcaa tctagaactc tttctggacg
     3901 agtatcttga agagatacga gatctcgact tccagctggg attgcgcatt acgaaacttc
     3961 tggaagaggg tcccatagac atcgatacga tagatgaatc gggtatcgag gatatcgaag
     4021 gattcgttct atcaatgctt tcagcgctgg aaaaaggtac aatagatcaa ctggatacga
     4081 acgacgaaaa cctgaaagaa tacttgaagc tgctagttta aggaggtttg aatggacagg
     4141 atagaggagc tgcttggaag attcatggaa actatacaga gcagaaacat cgatcagatc
     4201 gacgaggcga ctttgaatag aatccttcac gaagaattcg acgtctccga gaggctggag
     4261 cttgaagaag ctcttggatc gcttcttgga gaagtttcaa agatggcgga ggatccgaaa
     4321 gagtacttct tcagaaacga tgaagaaaaa ctcatggagt atctcaaaga aaaagaagaa
     4381 aaagattgat ttacttgatc gattaagtgt ataatcgctg tgaagggtta cttcagcgca
     4441 gagtatagcc ggtgtgaagc accggtcttt tttatttcat cagcgccctc tctgccgtct
     4501 tccatgctag agagacgcac ttgattctca tcggatactc tctgagtctt tcgaaaagga
     4561 cagcgtctcc aagaagattt tcgtcaaaga tttcacctcg cagcatttta ctgaaattgg
     4621 acagcgtctt ccgggcctct tccagggtct ttccttcgag aagatctgcc attaccgaag
     4681 ccgatgcctg acttatggcg cagccgtggc cgtcgaaagc tagagaggtt atcttcccct
     4741 ctttcattcg tacaaagaga gttatctcgt ctccgcacga gagattcgcc cccttttcca
     4801 tgaggtcgct ctctttgtcg atgcctctat gaccggagtt tctgtaatgg tgcattatga
     4861 aatcggaata gatatcatcg aaactcatcg aaccacctcc cggcctcttc aagagaatct
     4921 gcgagtctat ctacatcttc gaatgtgttg taaaggtaaa aggaaaccct gcaggccgag
     4981 acgactccaa gctctttcat ctgcaattgg gaacagtgat gaccactcct cagggcgacg
     5041 ttcatttttc ttcccaggaa ctcggcgata tcgtgagaat gtaccctgct gtgagtaaaa
     5101 gtaataaccg ctctgctgga ttcggggctg tggattctgt aaccaccgat ggcctcgatt
     5161 ttctctcttg ccatggcaac cagattagaa acatgggcgc tgactctctc cattccggcg
     5221 ttttccagat actcgacggc agccatcatt gagatcgcac cggagatatt ttgggtaccg
     5281 ccttcgaatt tcgcgacccc ttcggcgaaa gtcgtctcct cctttccaac catgtcaatc
     5341 atctggccgc cgaaaacaaa gggattcagt tcgcccagaa gtttctctct gccgacgagg
     5401 cagcctatac ctgtttcggc gagcatcttg tgaccggaga agacgaggaa atcgacgttg
     5461 ctttcgccga aagaaaactt ctcatgcgga atcagctggg cgctgtcgag caccatgacc
     5521 ccgccggccg aatgaacgag tgatcctatc tcttcgaaag gaggtctata acctgttgaa
     5581 ttaaccagac cggccagaga gaagacgaat ctgccgtcca tttgctggag agtctcgtac
     5641 accagcgaca tatcgagaga gttttgtctt accgggagcg gtatgaagcg taatccattt
     5701 ctcctggcgt gatactgcca ggggacgaaa ttgctgtgat gttcgaagac cggtacgatg
     5761 aaggtgtcgt aatctccact gatggagagg gaagaggcga gtacgttgag accttcggtc
     5821 gaacctttgg tgaagaccaa ctcgccaggc cagcagccca cgaagccgct caaccttctt
     5881 ctgctggatt cgtacagttc ggtagccgct tcggccagtg gataaacgcc cctgtgaacg
     5941 ttcgaattgt tcctttcgta aaaatccctg accgctgcga gaacggccga cggtttctgg
     6001 gttgtggcgg cgctgtccag ataaaccagc gaagggtcgt gcgagaatat ggggaagtct
     6061 cctttgagtc ccttgaaata accgttcaga tcttcaggcg aaaacacggt ccaaccctct
     6121 cgaatgatcg ggaaagagtt ccacaacttt tccaagcacg gggtcgaaag tccctttgat
     6181 tatgaacttc agggcctctt ccctggtcag tccgcgcgac atgaggtaaa agatcttttc
     6241 ctcgtccagc gatccgaccg ttgcggcgtg agacgcgttc acttcgtttt catcgaccag
     6301 aagggagggt atggcatccg ttctggcctc gcgggatagt accatcacac tgctgtgctc
     6361 cttacctgtg gctcccctcg aacccttttt aagatctacc gttcctctga aaatggagtg
     6421 gcccctgtcc atcaccacac cggtgccgtc tatgatcgcc tgcgatcggg ccgattttac
     6481 ccgcgcgatg tactgtaaat cgatcttcga gtctccgtaa gccatgaaga ccggttcgaa
     6541 caccgttcgg gattcttcgc cggcggcgac tcccatgtgg taactggcaa gaaccccgcc
     6601 gccgtaagaa acttcataca cgttcaagcg ggcaccggcc tcgagcaggt agaaattgtt
     6661 caaaaaacta aaatcgcttt tggagaggcc gttgacgacc agcaggtcta tggaggactc
     6721 tctcttcaga agaaaccggt tgttctgaag agaccacttt ccccgtccgg tcgagttcag
     6781 tactatcttc atctttgaac cttttcctgc tacgaagaga ttgcttgaaa tgcttgaatt
     6841 catctcctct ctgttgaata tgtaaggttc tttgagatgg attccggaag ggactgaaac
     6901 gaagtaaccg gtatcgaaaa agatatctcc caggagcaac agcttcctgt cagatccctc
     6961 gaaatccaga ttatccagga gctcaacttc ttcgctggta agccacttaa tcggtttcac
     7021 atatggatcg ggatagtgtt ttccgttttt cggagagaca ggcggaagga ccatcttact
     7081 tatatctact ctcttccagc gaggaaagcc gagttttctg tattcggagt accggttgct
     7141 tacgaaaacc gacatggcgt ttccgagagg ttcggatgat acgaggaaat ctcggtccct
     7201 gtagtccgga atttcgagct cccgcctgag tggcgagacc attttgtatt tgttgtgttc
     7261 gagattcaaa gtcttctcca tacgacacct catccgatac tgctttctat ctccaggtca
     7321 atcaaacggt tcaattccac cgcatattcg actggaaact ccctggaaac cggttccatg
     7381 aatcccctta cgatcatcga tctggcctgc gcttcagaaa gtcccctgct catcatgtag
     7441 aatatctgct cctcgcttat cctgccaatt ttggcttcgt gacctatgtt agccgaatcg
     7501 ttttcaactt ctataatcgg tactgtgtcg gaccttgaat cgttgtccat catcagggcg
     7561 gtacattgaa cggaagattt cgcattgtcg gcctggcgag agatttttaa ggctccacgg
     7621 taaaaagccc atccgccgcc ggtgcttatg cttctggcat cgaccacgga actggtttcc
     7681 ggagccagat ggatcacttt tgaaccggta tccatatgct gtcctgggcc ggcgtaagtt
     7741 atacccagat gttcggccct ggctcttctc cctttgagaa tggttgccgg atagagcatc
     7801 gttttcatac tgccgagcga accggatatc cactccatag ttccgtcctc ttcaactatc
     7861 gcccgcttcg tgttcagatt gtacgtattc ttcgaccagt tctgtatggt ggagtacctg
     7921 accttcgcgc cctttaagac gaagatctcc accataccga cgtgcaggtt caattcgttg
     7981 aatctggggg ccgaacagcc ttctatgaac tggaggttag aaccttcgtc ggcgatgatc
     8041 agggtgtgtt caaactgccc ggaactggcc atgttcatcc tgaagtaggc ctgcaaaggc
     8101 aggggtacgg aaacgttttt cggaacgtac acaaaggagc ctccgctcca gacggcgccg
     8161 tgcagggcgg agtatttgtg gtctgaggga gggacggcct tcatgaagta tttctccacg
     8221 tattcggggt attctttcac ggccgtttcc atgtcgagaa agattatccc gagctcctcg
     8281 aggttctttt ttatgttctg ataaactacc tcggaatcgt attgagcgcc aactccggcc
     8341 agagagttcc tctctgcttc agggataccg agcctttcga aggtttctct tatctccttg
     8401 ggcacttcct cccaggaacg gtttcgctcg gaaccgggct tgagataggc tactatgctg
     8461 gagatctaga tccccagttc ccaccccgaa accgggttct agcgagcggt tgaatatttc
     8521 aaaggactcc agtcttttcc gaagcatcca ttccggctcg tccttcttcg tcgatatctg
     8581 tcttataagc tcctcgtcga aacccttttc gcttatataa ctgtaatcgg ttgtggagac
     8641 gaaatcgaat ctcgaactat ccagaaacag gtctctatcc atatttacgc ctccctgtaa
     8701 gatttccagt tcctttcata gcctctctct tcaatatctc ttgccagttc tgggccgcct
     8761 gtcatgacta ttgaaccgtc cacatatatg tgaacgtagt cgggttcgat atactcgaga
     8821 agtctctcgt aatgggttat cagtataact ccgttttccg gggatctgaa attccttata
     8881 gaggtggcta tggatctcat ggcatctaca tcgaggcccg agtcgatctc atcgaggatc
     8941 gccagatcgg gtttcaaaaa atgcatctgc atgatctcgg ttttcttctt ctcaccgccg
     9001 gagaagccga ggttgagata cctgtctatg aactctctgc cgatgcctat ctcgtctgcc
     9061 agcctctcga gttctctgct ctttttcaaa atcggctcgt cgtttttcag gttgtgtgag
     9121 gccgatatca aaaaactgcg gagcctgatt ccgcttatct cctgaggata ctgaaaggac
     9181 ataaatatgc ccagtctggc cctttgatcg acggagagat cgttgatcgt tttgttcttg
     9241 aaacgtattt tgccggatgt aatttcgtat ttcgggtttc ccatcaccac attggccagg
     9301 gtagactttc cgcttccgtt tggacccatt atggcatgaa gctccccggt cctgactttg
     9361 aggttgactc ccttcaagat ctcctttccg tcttctctga gtgcggctct caattccttt
     9421 atatcgagca ggttcaattc gatccctcca aagtgggtat tatttgtcaa cattataaca
     9481 tattccgaca aaatatagca gtaataaaat cacttcatag cttcaaactg gcagttgaag
     9541 gttatataat ctttctatct cggaggtgga gtatgttcta tgtgattacg atcggagtca
     9601 tcatgggtct tgcaaatctc ataccgggag tcagcggcgg cactgttgtg ctgctcggag
     9661 gtctttacga gaggtttgtt ggtgctgtcg cttctctttc cgaattcaaa ttcgaaaaga
     9721 agcagcttat gttccttgta cagctcttcg ccgggataat tattggactg ctcgcctttt
     9781 ccggtctcat cgagctgtct cttacaaatg tcccttcgtt gatgtacgga atcttctgcg
     9841 ggcttgtggt gggaagtatc ccggtagtgc tgaaaaatat aaagaagttc acccccgcct
     9901 cggctatatg tttcgcggtc ggtgcggtcc tggtgctgac tatggccttt tccggagcca
     9961 gacccgggga cgatgcactc ctgacccatt ctcttacagc ctttctgtac gatttgctcg
    10021 ccggattcgt gggtgcggcg gccatggttc tccccggact cagcggggcc ttcgtgctgc
    10081 tcttgatggg tgagtactca agggcaatat cggccctcaa cgaccgcgac ctgttgataa
    10141 tacttttcat aggactggga gttgttgggg ggatagtttt cataagcaaa cttctgaagt
    10201 cgttgatgaa aaaacgtcaa aacgagactt tctcattttt gctcggcctg atgattggtt
    10261 ccattcccga ccttctcacc aggaccggtg aagctacgcg tttatcgttg ctgattccag
    10321 gagtggcgct gggggttttt ctctcttacc ttctcgccgt ctttgaaaga aaaaacagaa
    10381 aaagcccatc ttctgcggtc gatagtaacc gacaatagca atcctattga cgtagtctcg
    10441 ggcggactcc cggagagacg gttcctgata atttctcgaa tatcgctgtt ctatggcgtg
    10501 ctcggtggga tttgaaccca cagtctctgg atccggagtc cagcgctcta tccgttgagc
    10561 cacgagcaca tgtgcacggt acaattgtct gccagctcga tgtgcgacgc gggcagctta
    10621 tccccttcag tgactactat tatatcgtaa gttgagaagt cgatatcgtc aagaaggttt
    10681 ttcggtgtga agcctttttt caagaaggag aaatagatct gtggatcttc tacatataca
    10741 tccatttctt ttgtcgaatc gagcccctct atctttgaag agagtggcgc gtagagaagc
    10801 gcttttttgc cagtatctat cggcggaaag tttcgacata cgggaaagag atccctgacc
    10861 tcccagtttg cagtggttcc gcgagagtag tttctcagta tttccatggc gctcagcata
    10921 tcacgccggg tcgagaaact cagtccgctg tttttcgcga ttctttcgga ggatttcgtt
    10981 gtgagtcttt catcggtaag tatcaccctg cccgaaaata tcttcttcac tttttcggcg
    11041 aatgagacgg ctttcatcgc cagctggttg aaccgacccg acatagaaat tggcagccct
    11101 atgaccgtga gatctatctc tctttgagag aagatttcct tcagactttt gcgataatct
    11161 ctgtgctcaa tagtctcgag cggaatctcg acaccttcta tctggatcgc cagtccgatt
    11221 ctcaccgtgc cgaaatcaat tcccagtatc ttcataaaac tctctcacgg gatttgaaga
    11281 gcagtgaact atggagagcg gctccagacc aatttgctcc agaagctcaa ggcaacactc
    11341 cagattaccg gaagaaacgg caatggatat tgaacagccc ggaaaactat gcggcggggt
    11401 ggggaagagt ttggcggtga ttcccgcctc cctcaacgct tcaagggaag ggagagcagt
    11461 tccctggggc attaaaagca gtgtg
//
LOCUS       DAOOLY010000050        11284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6467_length_11284_cov_4.728115, whole genome shotgun sequence.
ACCESSION   DAOOLY010000050 DAOOLY010000000
VERSION     DAOOLY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_11284_cov_4.728115"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1009)
                     /locus_tag="PLD15_04045"
     CDS             complement(104..1009)
                     /locus_tag="PLD15_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB63105.1"
                     /translation="MIFVMIVLSVFAFLLLAGFVFSSKVIKPNCVPYEETYRMEIDKG
                     RFDPVWYDKLKKEEHWIDSPYGYKLHAIYLPCDASKKTVIICHGITYSLFGSMKYARI
                     FLDLGFNVMTYDHRNHGKSGGKDTTFGHYEKYDLKAVKEWVLKRTGEGTIVGLHGESM
                     GAAIALQYLAMDENISFCIADCGFSDLKELLKIRVNNDFHLPAFPFMDIAALITRIRT
                     GMSFADISPVKAVGTVKTPVMFVHGSEDWYVPAFMSRKMYEAKKTGSRKLLVVPGAAH
                     ASSIATDPAGYTDEIRSFLEQYGLS"
     gene            1147..2754
                     /locus_tag="PLD15_04050"
     CDS             1147..2754
                     /locus_tag="PLD15_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63106.1"
                     /translation="MKSEHWKIGHSQLLLYVFFGLAFLVPSLIILNNTPSILNSESYF
                     DSLKSIAGITVLIQAALLATTVIVTMLSAIMISGTYSTRLLYMFARFPDIYFVACLFV
                     FNVVFGTLLLASFPQAGSAWTKAFLSSGLLAISFFVPYLIRLLGIMQLENILRYLKAK
                     GVNTTRADETVRILKILVHFTERAVNENDRDAFCAVVSAASEVTSSAIYRGAGSENEI
                     SKRIRNLVDNMRSYTMELMRRGQSETVISLLQTYEDLGVALQEVNENWDCLVMELLKD
                     LQYIEQLSLEVDLRNRVSFQKKIWKSIIAILKAGPVYSAASEIFSSSFVSLNNLLNSG
                     CLNLASKDDLSSVLQTIKDMSEIEGRFFKTKILPDSRWELVLAGLSEQKTNREIMEEL
                     RKAIEKSIKGYVRNGQCSLAFERLQKIYENSRAFRGFFEGFFDASLLNEFIGNACPVK
                     ITEAAIGTMRDFILDNLSIDKSFLADTAVRLSNLESTGIKRTALEIFRDLLSRLDAMS
                     KTCTRDKKIESNRTREIVEKEIFERLA"
     gene            2772..3260
                     /locus_tag="PLD15_04055"
     CDS             2772..3260
                     /locus_tag="PLD15_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63107.1"
                     /translation="MVKLENMSPADFDAYLEKLTANYAKDNVRSGRWTREEALEKSMN
                     QINTLLPRGIDTQDHVFFSILDEGTGERAGYIWLNIAPGEGPKKAFIYDLIIFEEFRK
                     MRYGRAALAALEEYAKEKRIASISLHVFAHNAAAVSLYRKMGYEVTSMNMTRNVSDAQ
                     EG"
     gene            3265..3639
                     /locus_tag="PLD15_04060"
     CDS             3265..3639
                     /locus_tag="PLD15_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB63108.1"
                     /translation="MAKRNSPIVYWEINAKDGPALAEFYRDVFEWESSVDDTGFYSFK
                     SGSAGGIDGGIFTGKGLLPPHRALYIEVEDIKIVLERVARRGAEILLGPFESGNAILA
                     FFRDPEGHMLGLIERKRIESVK"
     gene            3636..4295
                     /locus_tag="PLD15_04065"
     CDS             3636..4295
                     /locus_tag="PLD15_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB63109.1"
                     /translation="MKPGESPFFASLSGDELDTLLKASRTVSVERGELLYTTDDLCDC
                     LTVVLSGGLKVSKLLPSGNEQIIKFLGEGDLLGEGFVFSRGPYRTNVVAERPSEVLEI
                     PLRVLLKAFKNERFLLSFLGSLSEKIDNLSDLIEILSLRKVRQRVAMYLIDKSTRENS
                     ETFALDKTRSEIAGGLGTVREVISRALSDLQREGIISLEGRKVKILDPRALERILPGK
                     Y"
     gene            complement(4315..5184)
                     /locus_tag="PLD15_04070"
     CDS             complement(4315..5184)
                     /locus_tag="PLD15_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63110.1"
                     /translation="MIERVTEGNMKDFLSYCQKYGGEHDDSYLPGDDFAIDDDHPSYL
                     LYDEDRRVCGASSLMLEERYRRARKGRFMIFHSTQSGLDGYRGLLEAILAEATEAVRS
                     VYLFVPPGPEIQRVLERLGFEIERYSFVMQRKMTDFEIPVQEGISFVAFNEREHRELY
                     CRIVNSAFKKIAGHIDIRPEWVSAMIEKYNIPAEGIQLLYFEGTPAGLFFSEITEDGM
                     LEIGPIAVLPEYQGRGLGRTLLRRAVQLSQRLGLDSVLSVNAENEKALSLYLKEGFTK
                     VDTRICYGLNLNP"
     gene            5499..6674
                     /locus_tag="PLD15_04075"
     CDS             5499..6674
                     /locus_tag="PLD15_04075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63111.1"
                     /translation="MIFKEISDYREMIEVSALAFSFEKRDRDELEELFEQLVEGGRLL
                     YGTYDGETLVAGCVVYPYRMRLRESAVTMGGIALVCSRQDYRGKGGVRLLLSATLESM
                     RDLGYPVSVLYPFQRDFYRKYGWEVFDRWQLIKFSPSSIRDFDSGDIDARDMAFPDEE
                     SIEFYRRYASSSYNYVLRNQTDWRDRLSPVWSSQVAKRVVRFTRQSEVVGLMDYSINL
                     DRKEGSYFHSILTFVSKDEKVRQAMFSYIKSLSHQVNKIHLFGPMNCEIWPYLYDKPD
                     ECQLREGSMIRIVDIMKLDGLMLDSPDVKIALKIEDAQCPWNIGGIMLEIRDGVLNLT
                     KIPEEEAQLEIGIGAISTVIGGRESLRRMVELKMARILKDYNGEDIQKCDVFMNEPF"
     gene            6776..7441
                     /locus_tag="PLD15_04080"
     CDS             6776..7441
                     /locus_tag="PLD15_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPB63112.1"
                     /translation="MELHPSRVVFNLKRSLEYYLSLPLQYDQIEDDWPLILFLHGAGE
                     RGNDLSLVKKHGIPRVVNEMEDFPFVTVSPQCPENDWWLNRLEDLKYLIDTIVEQYRI
                     DESRLYLTGLSMGGFGTWHMAVEYPETFAAIAPICGGGLGILGFPERVIEIKHIPVWV
                     FHGEKDNIVPVEESRVLVRELKSAGGNVQLKIYPEAGHDAWTETYSNPELYEWFMNNR
                     RSA"
     gene            complement(7433..7780)
                     /locus_tag="PLD15_04085"
     CDS             complement(7433..7780)
                     /locus_tag="PLD15_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63113.1"
                     /translation="MSFALKRGGRPKINRIRNNFIRASVLLAVVTPVSIYGIFGFMNA
                     SKRLEGFGWLLPLLIVIWALVGAIWLYSFYMYRKALQNARSYYTGLRSRRLKASGSSF
                     DRSIIVPIKMKSS"
     gene            8185..>11284
                     /locus_tag="PLD15_04090"
     CDS             8185..>11284
                     /locus_tag="PLD15_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB63114.1"
                     /translation="MNTLSFSLEEIFKGIGSFLKDRYPTLFDKYRPNLEVIERLVEPE
                     RSSLVRFSFKYNGRHEEIRFKCLEPDSRGVYYMDENKYSSPLVLVRKSREEKLSELDL
                     EKHLAIPGYFLSLALKMADVLRYLSKNGESGDIEKVKDAAECIFNRQKSGVKYHRLEP
                     SGRSDENKISDRKGEDSHGKFIWIKVPVLFELQDSNDLLKVSDLRKVVVPGDRVPESM
                     RRPHSSHIGKLDLLETPESEQIGMTLFMASGASYDAEKLEIIESKDGKEGNFSPSTRM
                     VPFIMYSDGTRVTMGGKNLKQAVRVVGSEKPLITTPVYDEIDMNIGVNALVGYALYWG
                     LNFEDGIVCSESFAEKMAIDEVRSMTFQDFVPVTPDSKVKVAGKSKVVIESEKAGIKV
                     EYAFRSSGSEVGKGDWLFKREVFQKREEGWSKIKGSYSAECYSSLYPGVIQSNASTDQ
                     IRLRRFHKIIKKDMKEKDLSDTGMVEVTVKVGTRKPLEVGDKITGRHGNKATISKILP
                     DSEMPSVELKGQRRPLDLILSPFGVITRMNLGQLLETQKSLEGEYVDEPFKPLDIAEK
                     LKENQPQLFLSFPDRKSFKATVGYQYFVRLDHCVRDKLHVVSEAQVSPITGQPVKGKR
                     RDGGQRIGEMEFWTLFDHNTINTINALSITNTEDPSNFKEDYFWTFRALVNTYKDVDP
                     QTIAEPCSIRFQKPKEIDVKENCSGNEKEKKIAARKKFLRGIRPSKKEKNPGSAKKKL
                     EKKYLLTKNGYLRRYMLGRRIHYSARATITPVTDIDIDHVYLPLEMALEWLKELKELN
                     VDPSNRPAAAARATDLAREKGLVVLLNRQPSLHKHSISAAKPLFWEHRTIGLPIMLCE
                     GFGADFDGDTMAVYLPIDQGEYTGEELKRMLPSFSPYRVGNGELVYSIDQDLVYGEYV
                     RSGLKKSEVKNEVLNIVRKAKNLPAELLKWQKETLDASMDSTLSISFAELATLSGNAK
                     KIKESGSRGKEKNFEEIPKFAKGLSHDEYIVRPGDLDPERTIASRSRAGLMDKKLHVA
                     QAG"
BASE COUNT         2985 a         2732 c         2966 g         2601 t
ORIGIN      
        1 ccccgatttt cgcgtctctc tcttaatctc gtgttttcga tactcctctc gcctcaggtc
       61 tttaccaagg acggctcttg tgctcttcct aagggctacc gactcatgaa aggccgtact
      121 gctccagaaa gctcctgatc tcatcggtgt atccggcggg atcggtcgct atcgaggagg
      181 cgtgcgccgc tccgggtaca acgaggagtt ttctagagcc ggtctttttg gcttcgtaca
      241 tttttcggct catgaaagcc ggtacatacc agtcctccga gccgtggacg aacatcaccg
      301 gggtcttgac tgtcccgaca gcctttacgg ggctgatgtc ggcaaagctc atgcccgttc
      361 tgatacgcgt gataagagcg gcaatatcca tgaagggaaa ggccggaaga tggaaatcgt
      421 tgttgactct aatcttcaac aactctttga gatcggagaa tccacaatcg gctatacaga
      481 acgatatgtt ctcatccatc gccagatact ggagcgcgat cgccgccccc atcgattccc
      541 cgtgcaggcc gacgatcgtt ccctctcccg ttctcttcag gacccattct ttgacggctt
      601 tcaagtcgta cttctcgtag tgaccgaaag tggtgtcttt accgccgctc ttgccgtggt
      661 ttctatgatc gtaagtcatc acgttgaacc cgagatcgag aaagatcctg gcgtatttca
      721 tcgatccgaa gagcgaatag gttatcccgt ggcagatgat cacggtcttc ttagaagcgt
      781 cgcatggaag gtatatggcg tgcagcttat agccgtaggg cgagtcgatc cagtgctcct
      841 ctttcttcag cttgtcgtac cagacagggt cgaacctgcc tttatcgatc tccattctgt
      901 acgtctcttc gtatggaacg caattcggct ttatgacctt gctggaaaaa acaaaaccgg
      961 ccagaagcag aaaggcgaaa accgacaaaa cgatcatgac aaaaatcata tctctccctc
     1021 catcgattaa accgccggta cggttcaggg tttagaatat tactggaacg gtataaagcg
     1081 gaaaactgtt tgctcaacaa caaatcgatc ttctccgtca aacgcgcaaa aaggaggcgt
     1141 acctgaatga aaagcgagca ctggaaaatc gggcattctc agttgctgct ttacgtcttt
     1201 ttcggtctgg catttctcgt tccttctcta atcatactaa acaatacccc gtcgatcctc
     1261 aattcagaaa gctatttcga cagtcttaaa tcgatagcgg gtatcactgt actgattcag
     1321 gccgctttgc tggcaacgac cgtgattgta acgatgcttt ccgcgatcat gatctccggc
     1381 acttattcga ccagacttct gtatatgttc gcccgtttcc ccgatattta tttcgtcgct
     1441 tgtctgttcg tgttcaacgt ggtctttggt acccttcttc ttgcgagctt tccacaggcc
     1501 ggatcggcct ggacgaaggc ctttctttcg agcggccttc tcgccatatc cttctttgtg
     1561 ccttatctga taaggcttct ggggatcatg caactggaaa acatccttcg atatctgaaa
     1621 gcgaagggag tcaacaccac gagggcagac gagacggtga gaatactcaa gatactcgtg
     1681 catttcaccg agagggccgt aaacgagaac gaccgggatg ctttctgcgc agtcgtgtcg
     1741 gcggccagcg aggtgacatc gagcgctatt tacaggggtg ccgggtcgga gaatgagatc
     1801 tcgaaaagaa taagaaatct cgtagataac atgaggagtt acactatgga gctcatgaga
     1861 aggggccaga gcgagaccgt aatttcgctt ctgcagacct acgaagacct tggcgtggct
     1921 ctgcaggaag tgaacgaaaa ctgggactgc ctcgtgatgg agcttctcaa ggatctccag
     1981 tacatagaac agttatcgct cgaagtcgat ttgaggaatc gagtgagctt tcaaaagaag
     2041 atatggaagt ctataatcgc catactcaag gccggtccgg tctattctgc ggccagcgaa
     2101 atattttcca gttccttcgt atctctgaac aatctcctca attccgggtg tttaaacctg
     2161 gcctcaaagg acgacctctc ctcggttctt cagacgatca aagatatgtc ggagatcgaa
     2221 ggcaggtttt tcaagacgaa aattctgccc gatagccgct gggaactggt gctcgccgga
     2281 ctgtccgagc agaaaaccaa cagagagatc atggaggagt tgagaaaggc catcgagaaa
     2341 tctataaaag gttacgtgag aaacgggcag tgcagcctcg cattcgaaag gcttcagaag
     2401 atatacgaaa actcgcgtgc tttcaggggc tttttcgaag gttttttcga tgcctctctg
     2461 ctcaacgagt tcatagggaa cgcctgcccg gtgaagatca cggaggcggc tatcggtacg
     2521 atgcgggatt tcattctcga caatctttcc atcgacaaga gcttcctcgc cgataccgcg
     2581 gttcgcctat cgaacctcga atcgacgggg atcaaaagaa ccgcgctcga gatcttcaga
     2641 gatcttctat cgcgtctaga cgcgatgagt aaaacctgta cgcgcgataa aaagatagaa
     2701 tcgaacagaa ccagggaaat agtggagaag gaaatcttcg agagactggc atgagtcttt
     2761 tgggaggtct tatggttaaa ctggaaaaca tgtcgccggc ggatttcgat gcgtaccttg
     2821 aaaaactcac ggccaattat gcaaaagata acgttcgatc cggcagatgg accagagaag
     2881 aggctctgga gaaatcgatg aaccagataa acactctcct gccgcgggga atcgacacac
     2941 aggaccacgt ttttttcagc atattggatg aagggaccgg cgaaagggcg ggatatatct
     3001 ggctcaatat cgcccccgga gaaggtccga agaaagcctt catatacgat cttataatat
     3061 tcgaagagtt cagaaaaatg aggtacggcc gtgccgccct cgccgccctg gaagagtacg
     3121 cgaaagaaaa gagaatagcc agcatctcgc tccatgtgtt cgcccacaac gcggcggcgg
     3181 tttcgcttta caggaaaatg ggttacgaag tcaccagcat gaacatgaca aggaacgtat
     3241 cggatgcgca ggagggatag gaccgtggcg aagagaaata gccccatagt ttactgggaa
     3301 atcaacgcaa aagacgggcc ggccctggcg gagttttacc gggatgtctt cgaatgggag
     3361 agctctgtgg acgataccgg gttctattca ttcaagagcg gaagcgccgg aggcatagac
     3421 ggcggaatct tcaccggcaa agggcttctc ccgccccaca gggccctgta tatagaagtc
     3481 gaagatataa agatagttct cgagagagtt gccaggcgcg gggccgagat tctactgggt
     3541 ccgttcgagt cgggtaacgc gattctcgcc tttttcagag acccagaagg ccacatgctg
     3601 ggtctgatag aaagaaaacg gatcgagagc gtgaaatgaa acccggcgag agccctttct
     3661 tcgcttcact gagcggagat gagctcgaca ctcttttgaa ggcttcgagg acggtctccg
     3721 tagaaagggg cgagctcctc tataccaccg acgatctctg cgattgtctc acagtggtac
     3781 tctcgggcgg tttaaaagtg tcgaagcttc ttccatcggg aaacgaacag atcataaagt
     3841 tcctcgggga gggcgatctc ctcggcgagg gcttcgtctt ttcgagaggc ccgtaccgaa
     3901 cgaacgtggt cgccgaaaga ccttcggaag tcctcgagat cccgctcagg gtcctgctga
     3961 aggccttcaa gaacgagcgg ttcctcctga gctttctggg cagcctttcc gaaaagatcg
     4021 acaacctttc cgacctcatc gaaatacttt ctctcagaaa ggtaagacag agagtggcca
     4081 tgtacctgat cgataaatcc acccgcgaaa attcggaaac cttcgctctc gacaaaacca
     4141 ggagcgaaat cgccggcggt cttggaaccg tcagggaagt catttcccgc gccttgtcgg
     4201 atcttcagag agagggaata atatccctcg agggaaggaa agtgaagata ctcgatccga
     4261 gggcactcga gagaatcctg cccggcaaat actgagcctg ccgatctgat agaatcatgg
     4321 attcagattc agcccgtagc agatcctggt gtcgaccttc gtgaacccct ccttgaggta
     4381 cagagaaagg gccttctcgt tttcagcgtt cacggagagc acgctgtcca gacctaatcg
     4441 ctgcgacagt tgaacggccc tgcgcaacag cgttctaccc aatcccctac cctggtactc
     4501 aggcagcacg gctatcggcc ctatctctag cattccgtct tcggttattt cactgaaaaa
     4561 gagacctgcg ggagtgcctt caaagtaaag caactgaata ccctccgccg gaatgttgta
     4621 cttttcgatc atcgccgaga cccactcagg tcttatatcg atatggccgg cgatcttctt
     4681 gaaagcgctg ttgactatac ggcagtagag ttctctgtgt tctctctcgt taaaggccac
     4741 gaagctaatg ccttcttgaa cggggatttc aaaatccgtc atttttctct gcatgacaaa
     4801 tgaatagcgt tcgatctcga agcccagcct ctcgagcaca cgctggattt ccggcccggg
     4861 cggaacgaag agatagacgc tcctaaccgc ttccgtagct tcggcaagga tggcttcgag
     4921 caaacccctg taaccatcca gtcccgattg agtggagtga aatatcatga accttccctt
     4981 cctggctctc ctgtagcgct cttccagcat gagcgaagag gcgccgcaaa ctctccgatc
     5041 ttcatcgtag agcaaatagg aaggatgatc atcgtcgatc gcgaaatcat ctccggggag
     5101 atacgaatca tcgtgttctc ctccgtactt ttggcagtag gaaagaaaat ccttcatatt
     5161 tccttcagtt acacgttcta tcaagttcgc cgcctccctc taagaacggt tgttggttgt
     5221 gcgttgtggg taagagcagg ataccataca gaatcacagc cttcatgacg agaagattgt
     5281 aagactgtca tcgcgtgaaa aaccgggact gacaaatctc ttcatgtctg tcccattttt
     5341 cgcgtcagtc agtctctctc tccccttctc gtggtcctta accaacatcg ctcgacagtt
     5401 ctttccgctc ttccgggtag tgttcttgcg ctgtggtctt catgatacaa tgttcgtcgt
     5461 gacacatatg caacaaatcg agaatagggg gagataaggt gattttcaaa gaaatatccg
     5521 attacaggga aatgatcgag gtatcggctc tggccttttc cttcgagaag agagaccgcg
     5581 acgaactgga agaactcttt gagcagctgg tcgagggggg ccgcttgctt tatggaacct
     5641 acgacgggga gaccctggtg gccggatgtg tcgtatatcc ttacagaatg aggttgaggg
     5701 aaagcgcagt tacgatgggc ggaatcgcgc tcgtctgctc gaggcaggac tacaggggca
     5761 agggcggcgt caggctgctt ctgtcggcca ctctggagtc catgagagat ctcggttatc
     5821 ccgtatcggt tctttatccc ttccagaggg acttctacag gaagtacggc tgggaggtct
     5881 tcgacaggtg gcagctgatc aaattctcgc cctcaagcat aagggatttc gattccggcg
     5941 acatcgacgc ccgggatatg gccttccccg atgaagaatc gatagagttt taccggcgct
     6001 acgcctcctc cagttataat tacgtcttga ggaaccaaac cgactggcgt gacaggctat
     6061 cgccagtatg gtcctcccag gtcgccaaga gagtggtcag gttcaccaga caaagcgaag
     6121 tcgttgggct gatggactac tcgatcaacc tggaccggaa agaaggcagt tactttcatt
     6181 ccatccttac ctttgtgagc aaagacgaga aggtaagaca ggccatgttc tcgtatataa
     6241 agagcctctc ccatcaggtc aacaagatcc atctcttcgg gcccatgaat tgcgagatct
     6301 ggccgtatct ttacgacaaa cccgacgaat gccagttgag agagggatcg atgataagaa
     6361 tagttgatat catgaaactc gacggcttga tgctcgacag cccggatgtc aaaatcgcgc
     6421 tcaagatcga agacgcgcag tgtccgtgga acatcggcgg catcatgctg gagatacggg
     6481 acggggtgct gaacctgaca aaaatccccg aagaagaggc gcagctggag atcgggatcg
     6541 gggcgatttc gaccgttatc ggcggaagag agtcgttgag gagaatggtg gaactgaaga
     6601 tggcaagaat cctgaaggat tacaacggcg aggacataca aaaatgcgat gtcttcatga
     6661 acgagccttt ttgaaaaagg aaagcggcgc atgactttgc gagagtatat gattttatcg
     6721 gctcgccggg agtattgtgt tatcctctaa attaagaccg agaggaggtg gaagaatgga
     6781 gcttcatcct tcgagagtgg ttttcaactt gaagagatcg ctggaatact atctatcgct
     6841 acctttgcaa tacgatcaga tagaggacga ctggccgctc atactgtttc tccacggagc
     6901 cggcgagagg ggaaacgatc tatcgctggt gaaaaagcac ggcattccca gagtcgtgaa
     6961 cgagatggag gactttccat tcgtgacggt atcgccgcaa tgcccggaga acgactggtg
     7021 gttgaacagg cttgaagacc tgaagtacct gatagacact attgtcgaac aatacagaat
     7081 cgacgagtcc aggctctatc tcaccggcct ttctatgggt ggattcggca catggcacat
     7141 ggcggtcgaa tacccggaga ccttcgcggc gatcgcaccg atttgcggcg gggggttggg
     7201 catactgggc ttccccgaac gggtgattga gataaaacac atcccggtct gggtctttca
     7261 cggcgaaaag gacaacatcg tcccggtgga ggagagcaga gtgctggtgc gggagctgaa
     7321 atcggccggc ggcaacgtcc agttgaagat atatcccgaa gcaggacacg acgcctggac
     7381 agagacgtat tcgaaccctg agctctacga atggttcatg aacaacagaa ggtcagcttg
     7441 atttcatctt tatcggaacg ataatcgacc ggtcgaagga ggaacccgaa gctttcaggc
     7501 gcctcgatct gaggcctgta tagtagcttc tcgcattctg gagagccttt ctgtacatgt
     7561 agaacgagta aagccagatg gcgccgacca gagcccatat cacgatcagc agtggaagca
     7621 accagccgaa gccttcgagc cttttactcg cgttcatgaa accgaaaatc ccgtatatgg
     7681 agaccggcgt aaccactgcc agcaggactg aagcccttat gaaattgttc cttatccggt
     7741 ttattttagg tcttccaccc cgtttcaggg caaaactcat agaacttcac ctccgtacgt
     7801 ctttcacatt ccataacaat tatacacgct tcactttatc cgggttaccc gagaagatct
     7861 ctccaggtaa agatcccgac aactttgaga cagcccgaaa gacactattg ttcaacaggg
     7921 agccacacca cacactccta ttgcctgatc taaacagccc ctcttcggcg gggcgtgtgt
     7981 tagtgcaacc ggctccggcg tcacgaacag gttgagaaga ctggagttta cttcccggtg
     8041 tagaataaaa agacgcgagg cagagtattc atgctgcaat gtctgtggaa tattttttat
     8101 actttctagg atatttcatt tcaaatagat ataattgtgt taccgggaac ggattaagca
     8161 gttgattagt cggaggtttg cgcaatgaat actcttagtt tctcgctgga ggaaattttc
     8221 aaaggaatcg gtagctttct gaaagaccgt taccccactc tcttcgataa gtatcgtccg
     8281 aatcttgaag tgattgaacg gctggtagaa ccggagcgtt cttctctggt ccgcttttct
     8341 ttcaaataca atggaagaca cgaggagatt cgcttcaaat gtctcgagcc ggattcgagg
     8401 ggagtatatt acatggatga aaataaatac tcctcgcctc tggttcttgt gagaaagtcg
     8461 agagaggaaa agctgagtga actcgatctg gaaaagcatt tggctattcc cgggtatttt
     8521 ctctctctgg ctttgaaaat ggccgatgta ctgagatacc tttccaaaaa cggggaatcg
     8581 ggggatatag aaaaagtaaa agacgccgcc gaatgcatat tcaatagaca gaaatccggt
     8641 gtgaagtacc accggctgga gccttcaggg agaagcgatg aaaacaaaat aagcgatcgg
     8701 aagggtgaag atagtcacgg caaattcatc tggataaaag ttccggtttt gtttgaacta
     8761 caagactcca acgatttgct caaggtatcc gacctgagaa aggtggttgt cccgggagat
     8821 cgtgtgccgg aatccatgag gcgtccccat tcatcgcata tcgggaagct cgatcttctc
     8881 gaaacacccg aatcggagca gatcggaatg acactgttta tggccagcgg cgcctcctac
     8941 gatgccgaaa aactcgagat aatcgagtcg aaagatggta aggaaggcaa tttcagccct
     9001 tctacaagga tggttccttt catcatgtat tcggacggca ccagagtcac gatgggcggg
     9061 aagaacctca agcaagcggt cagggtagtc ggtagtgaaa aacccttgat aactactccc
     9121 gtgtacgacg agatcgacat gaacataggc gtcaacgccc tcgtcggtta tgcgctgtac
     9181 tggggactga acttcgagga cggtatcgtc tgctctgaaa gctttgccga aaagatggcg
     9241 atagacgagg tcagatccat gactttccag gatttcgttc ccgtgacgcc cgacagcaag
     9301 gtcaaagtag cgggtaaatc gaaagtcgtc atcgagagcg aaaaggccgg aataaaagtg
     9361 gaatacgctt tcaggtcgtc tggaagcgaa gttggaaagg gagactggct cttcaaaaga
     9421 gaggtatttc agaaaagaga ggaaggctgg tcgaagataa aaggctctta cagcgccgag
     9481 tgttatagct ctctctatcc cggagtgatc cagtcgaacg cgagcaccga tcagataaga
     9541 ctgagaaggt ttcacaagat tataaagaag gatatgaagg aaaaagatct ctccgataca
     9601 ggcatggtcg aagtaacagt gaaagtcggt accaggaagc ctctggaggt tggcgataaa
     9661 atcaccgggc gacacggcaa caaggcaacg atatcgaaga tcctgccgga cagtgaaatg
     9721 ccttccgtag aactgaaggg acagagaaga cctctagacc ttattttgag ccctttcggg
     9781 gtcataacca gaatgaacct ggggcagctc ctggaaacgc aaaaatcgct ggaaggtgag
     9841 tatgtcgatg aaccttttaa acctctggat atcgccgaaa agctcaaaga aaatcagccg
     9901 cagctttttc tcagttttcc ggacagaaaa tccttcaaag ctaccgtggg ttaccagtac
     9961 tttgtaagac tcgaccattg tgtgcgggat aagcttcatg ttgtcagcga agctcaggtt
    10021 tctcctatca cgggccaacc ggtgaaaggc aagcgcagag acggtggtca gcggatcgga
    10081 gagatggaat tctggactct cttcgatcat aatactataa acacgataaa cgccctttcg
    10141 attacgaaca ccgaagatcc ctcgaatttc aaagaggatt atttctggac attcagggcg
    10201 ctggtaaaca cttataaaga tgtagatccc caaacgatag cagagccgtg ttccatacgc
    10261 ttccagaaac ccaaagaaat agatgtaaaa gagaattgtt cgggcaacga gaaagagaaa
    10321 aaaatcgctg ccagaaagaa attcctgcgt ggaattcgac cctccaaaaa agaaaaaaac
    10381 ccgggttccg ccaaaaaaaa gctcgagaag aaatacttgc tcacgaagaa cggttatctg
    10441 cgaaggtata tgctcggcag gagaattcat tactcggcca gggcaactat aacacccgtc
    10501 acggatatag atatcgacca cgtttatctg cccctggaaa tggccctgga gtggctgaag
    10561 gagctgaaag aactgaatgt cgatccttca aaccgccccg ctgcggccgc cagggcgacc
    10621 gatctggcca gagagaaggg gctcgtggtg cttttgaacc gccagccttc gctgcacaaa
    10681 cacagtatca gcgcggcaaa gcctttgttc tgggagcaca gaaccatcgg cctgcctata
    10741 atgctttgtg aggggtttgg ggccgatttc gatggagata ccatggccgt ttatttgccg
    10801 atagatcagg gcgagtatac cggagaagag cttaagagaa tgctcccttc cttctctccg
    10861 tacagagtag gcaacggcga actggtgtac tccatcgatc aggacctcgt gtacggagag
    10921 tatgtccggt cgggtttgaa aaagtccgaa gtcaaaaatg aagtcctcaa tatcgttcgg
    10981 aaagcgaaaa atttgcccgc cgaactcttg aagtggcaga aagaaactct ggatgcgtcg
    11041 atggattcga cattgagcat atctttcgcc gagctcgcga cgctttcagg taacgccaag
    11101 aaaataaagg aatcgggcag ccggggaaag gaaaagaatt ttgaggagat accgaagttt
    11161 gcgaagggtt tgtcgcacga tgagtacata gtcagaccgg gagacttgga ccctgagcgg
    11221 actatcgcga gtcgatcgag ggccggactg atggacaaaa agcttcacgt cgcccaggcc
    11281 ggtt
//
LOCUS       DAOOLY010000051        11216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6532_length_11216_cov_5.296568, whole genome shotgun sequence.
ACCESSION   DAOOLY010000051 DAOOLY010000000
VERSION     DAOOLY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6532_length_11216_cov_5.296568"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /gene="pheT"
                     /locus_tag="PLD15_04095"
     CDS             <1..1784
                     /gene="pheT"
                     /locus_tag="PLD15_04095"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB63115.1"
                     /translation="GEDPVLKVALNDTGCRRYSALLMDGVKIAPSPLWLKRRLVAAGL
                     RPINNVVDITNYVMLETGHPIHAFDVDRIGSTKILVRNARNGEELELLDGRKVELNES
                     DLLITNGETPLALAGIMGGEDSGIDVTTDRVLLEVAVFDPVRIRKTARRLGISTDSSY
                     RFERGVDYTDSIYVLKRLAGLMEELCRGRVASKIVDVGRIENPEGISLRKTFVTERLG
                     KELPDGEIERILTGLEFGVERRDGGWIVTPPAFRAYDTTQEVDLVEEIGRIYGYDRID
                     DELPRILPVSIGVPGYLKRQRKLKELMVANGFDEIISYSFMNPDEIRKVDEEISFVPL
                     QNPLSMDMAVMRPSMVFGMLSAASYNFRRQNRDLKLFEIGSIFSPETGRREYTAVGFA
                     AMGRENPLDFSDKRAIDFFTVKGVIEELFSLFGASPRFGQISRKWLEQGKAVSVIMDG
                     REVGFFGAFSRTLADSLYDIKSGELYVGELNLTLIDSMRNEFTGSQRISPFPRVSRDL
                     SFLVPSSLAYSELEGIIESSLEGTSFSEIRVSDIYRGKGVEQGFTSVTVTLDFESFER
                     TLTDDEVNDCITKVLDATSRAGVKLRG"
     gene            1777..2559
                     /locus_tag="PLD15_04100"
     CDS             1777..2559
                     /locus_tag="PLD15_04100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB63116.1"
                     /translation="MDDLILNSLNRKILAFLPQLWTDIDTVGFSVARKSSFKDIVTSI
                     DIEIENRLRLFLEELLPGADYLGEESSSSVRSGLMWIVDPVDGTTNFSKSNPHYCTQV
                     ALYKDEKIVLAVTYDHNRRELFHAIEGQGAYLNSKRIWVSKVDDVSEASSHVGLQYSS
                     ELSFKRVTERINRAIEACRAVRITGSACLDLAYVACGRADIFWEEALKPWDVASGILL
                     VREAGGEVTGCLDERFDIFKPDIFATNGSRRLAGQFKERVLY"
     gene            complement(2566..4476)
                     /gene="lnt"
                     /locus_tag="PLD15_04105"
     CDS             complement(2566..4476)
                     /gene="lnt"
                     /locus_tag="PLD15_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPB63117.1"
                     /translation="MKLILLSALLTALSMPGMVWGYLVWIALIPFFMGMRSARPLGGA
                     LKGFLFGFAYLMITHYWELPVLALNVPEVLDSFPNFIGVVAYVLMCLVIAVPFAGFGF
                     VYSIFQRFFKARPLAGALFAASFFTVFEGLRELGPLGFTNGRLSDALLNSQLGISQLL
                     ALGGPLLLVFVVVLVNHYLAHLCSDRKSGRVPLLITAVAMVALVNALIQSAIPLRPFD
                     KSSEKTLYALQTNISQQLKYYAPPEETLKVVLMGLEEIPEGSTVIMPEATFMSDIRIS
                     PTGTRLVNLARERNLEILVGFPIYNGENYNQVRFVNGDGFSGEFYAKVKLTPFVEFLP
                     WPKILGVFEFLKFLDYFYAGEEFTVFSVDGHRIGAQICFDSLYGEVARNLTLNGAGII
                     VISTNDGWFNIDTALQQHFSKGIARAIENRRYVLQVSNTGITAIIDPYGRVLKRLPTM
                     NETNTQYVIGAFQYLPGTQITPYTRFGNWFFFFSLVLGSALIILGGVFPVKHEDKAKE
                     ILSTFKRIVVVGFSKNPDKPSNSVPMYLKEKGYEIIPVNPSMEEYEGMKVYPDLKSVI
                     ADGVNLEAVEIFRPSAEAEEIAVEAIELGARAVWLQKDIRSEKARKLASERSVLYVED
                     KCMYVEHRRFFP"
     gene            complement(4478..5317)
                     /locus_tag="PLD15_04110"
     CDS             complement(4478..5317)
                     /locus_tag="PLD15_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPB63118.1"
                     /translation="MIRLIVIDLDGTLLNSDKSISRENEEAIMEALGRGVHVSISTGR
                     SYVSGHKYVEQLGISVPVSYQNGALVIEGYDGAMRIISQCLLGTAEAVEIYNRSRERG
                     LNSLIFFDFFKLPDISTAGLFDSPYRGYYAHNAYRIVLEEDPSKRIREPGIAEIAIEG
                     EEERIIEMIEAMRPGPDRVTAVKNDALESHAFYEFFGPGVGKSNGLQHILEHLKIDRE
                     EVAYIGDNYNDLDILKRCPLPVVMANAPDQVKEHAKIVTEKTNDESGVAEAIDRILNW
                     RRD"
     gene            complement(5321..6271)
                     /locus_tag="PLD15_04115"
     CDS             complement(5321..6271)
                     /locus_tag="PLD15_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HPB63119.1"
                     /translation="MEGNLIDTTIAEIREISRGERITVAFSGGQDSTLVAALASEALG
                     RDRVKLVNVCFGPYSYSRGLEIVVELADRLGLRLEFTPGYEAQEAVWKNGPSCNRCTR
                     HAKLSSMRNSALGIIATGANQSDTWGQTGIALKDGFYAPIRGWDKKLIGMALEELKIH
                     VPRIGEAPVREGCKLKHLLKIMANPSYHGYAVAISNEILLDCLESFPHTLANVKIAGP
                     LSKNIALVNVSPLPPVELRERITAMLGRVEEIDEVRWIDGETKLKILANPGIYNSEEA
                     RYWIERGRLAPEFAFPVSFEWTRSTNRRLETFQVVDSWRL"
     gene            complement(6498..7601)
                     /locus_tag="PLD15_04120"
     CDS             complement(6498..7601)
                     /locus_tag="PLD15_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB63120.1"
                     /translation="MEYRTLGKTGLKLSLLGLGGFHLLEIGVETVGKIVKKYLDAGGN
                     YFETARSYGDGDSERKLSSFLPKEGVIVATKTGERTFEGSQRQLMESLKALGRDHIDI
                     LFLHAVTENGDWEKIISSDGALKTLEWAKTEGLIRFAGITSHGYGGTLFRALNEYPFD
                     LFMTHFNYFDRFNFPEIEMKILPYALSNGIGILAMKPLADGYLWRSVEEAFRYVKTLP
                     VSCIVSGMNSPEHLDEDLSVLDGPSMTPFELDGLFSRAPELADYVCRQCMKCLPCPEG
                     INIPGFFLAEGRYDRQMLDGRIGKAPDYALRDRLAHWFSSEEQGRREYEVLSPGIDSC
                     TDCGICSQRCPYGLDIPRKLRIVKSKFDRGFVW"
     gene            complement(7619..8854)
                     /locus_tag="PLD15_04125"
     CDS             complement(7619..8854)
                     /locus_tag="PLD15_04125"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPB63121.1"
                     /translation="MHFLEEYLVKEVKPALGCTEPGAIAYCVSLAVGHLQDSVERVEV
                     ITSKSVYKNGMYVYIPGTGEKGNEVAAALGVICGDSSLQLEALKPCCGESVALAREMI
                     DSGRVTVRCDTSRTGIYIEALVKGFTHTARVVIKDHHTRVVLVERDGREIESDEGAVE
                     SRDIDFTVEDIFKAVDEMTDGEIDRIFEGINMNLEIAGYGLEKIVAPREKAEACESGS
                     LGYRIRKYCLAASAARMTGAPLPVMSSGGSGNQGIVATLPVVLVGMHYDKSRGEIARA
                     VAISHLFSSYLKKKLGRTAPICGCVTAAGTGASAGIVYLLGGDRSVMERAMITVLSNT
                     TGMMCDGAKESCALKAGLGGQEAYSCALLAIDDLGIDSAQGFIAPTLDKSIENIQLLM
                     SRGMSEIDNTIIELLEKRR"
     gene            9115..10560
                     /locus_tag="PLD15_04130"
     CDS             9115..10560
                     /locus_tag="PLD15_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63122.1"
                     /translation="MGIVESMKASMLRQAGKFVGSLVKNSSTENIARLFGTVATLSKE
                     PTKSGLKKLTQMAKDDHPMIKSWQKVFQNASPKAVEKAMTNLVVNEFALGEKIRQEKM
                     LEHEVVIPKLLVLSPTYACNLNCVGCYAGLYGRKYQLSKEEVSSIIRQANELGIYFFI
                     VTGGEPFVWPHLLEIFEEFNDSYFQVYTNGTLITKEVAKKLAELGNATFAVSVEGFEG
                     ETDWRRGRGVFEKIKTAWANLKEAGVIYGTSVTATRLNHDVMMKDEFWQFLKDNGVAY
                     AWVFQFMPVGMNPSMDLVPTPEQRYERFFKTEEQRLGGEFAFVADFWNHGFLTNGCLA
                     AGSKYLHINAKGYVEPCVFQQFAVDSIREKSLIEILKSPFFTAYKRMVPYSNNLFRPC
                     PIIDNPKVLRAMVNKFNAIPQHDGSENVVTELAGQLDELAEGWKQYADKLYYEEGFAE
                     THPAHRGVYDFEVRMRKYANNEEKLAIDKKP"
     gene            10583..11167
                     /locus_tag="PLD15_04135"
     CDS             10583..11167
                     /locus_tag="PLD15_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB63123.1"
                     /translation="MRARLSSKERIVRAARKAFADRGRDGVSMSEIAGIAGVKKALIY
                     YYFPSKEDLFYEVWRYSIDELEDHIFSEADGENVYLSKIKKILRSYIDFITSKNEVKK
                     IMDQERANLTREDIESWKRVRDRYLSLKNRLSTVINDAKTAREISKDVDPDSTADLIL
                     NGFSITEDPEGLESIREIIWRGLISNSGESTTPG"
BASE COUNT         2648 a         3041 c         2990 g         2537 t
ORIGIN      
        1 ccggagagga tccggttttg aaagttgcgc tcaacgatac aggctgcaga cgatactcgg
       61 ccctccttat ggacggcgtg aagatagccc cctctccttt gtggttgaag aggagactgg
      121 tcgccgccgg actgaggccg ataaacaacg tcgtggacat caccaattac gtgatgctgg
      181 agacgggaca tcccattcac gccttcgacg tggatagaat cggatcgacg aaaatcctcg
      241 taagaaatgc tagaaacggc gaagaactgg agcttctcga cgggcgcaaa gtcgagctga
      301 acgaaagcga tctgctgata accaacggcg agactcccct tgcccttgcc ggaattatgg
      361 gcggggagga ttcgggaata gacgtcacca ccgacagggt ccttctggaa gttgccgttt
      421 tcgatcccgt gaggatcagg aagaccgcca gaagactcgg catatcgacc gacagctctt
      481 acagattcga gcgcggagtt gattacaccg attccatata cgtcctgaag cgtctggccg
      541 gactgatgga ggaactctgc cggggcaggg tcgcctctaa gatagtggat gtggggcgga
      601 tcgagaatcc cgaagggatt tcgctcagaa aaaccttcgt cacggagcgt ctgggcaagg
      661 aactcccgga cggggagata gagaggattc ttaccggcct ggagttcggc gtcgaacgcc
      721 gcgacggggg atggatagtg acaccgccgg ccttcagggc ttacgacacc acccaggagg
      781 tagatctggt agaggagata ggaaggatct acggttacga tcgcatagac gatgagttgc
      841 cgaggatact gcccgtttcg atcggtgtcc cgggatacct caaaagacag cgcaagctta
      901 aggagctcat ggtcgcgaac gggttcgacg agatcataag ctacagtttc atgaacccag
      961 acgagataag aaaggtcgat gaagagatct ctttcgttcc ccttcaaaac cctctttcga
     1021 tggacatggc cgtcatgaga ccctccatgg tcttcggtat gctttcggcc gcttcctaca
     1081 atttccgcag acagaacagg gacctgaaac tcttcgagat aggctcgatc ttctcccctg
     1141 aaaccggaag aagagagtac accgcggtgg gcttcgctgc gatgggcaga gagaaccccc
     1201 tggacttctc ggacaagaga gcgatcgact tcttcaccgt caagggagtg atagaggagc
     1261 tcttctcgct cttcggagct tctccccggt tcggtcagat atccagaaag tggctggaac
     1321 agggaaaggc cgtgtcggtg atcatggacg gcagagaagt agggttcttc ggagccttca
     1381 gccgcacgct ggccgattcc ctttacgata tcaaatcggg ggagctgtac gtgggcgagc
     1441 tcaatctgac tctgatcgat tcgatgagaa acgagttcac gggctctcag aggatttcgc
     1501 ccttccccag agtatcgaga gacctgtctt tcctcgttcc ctcctcgctc gcctattccg
     1561 agctagaagg gatcatcgaa tcgagtctag aaggcaccag cttcagcgag ataagagtct
     1621 cggacatata caggggaaag ggtgtcgagc agggtttcac tagcgtcacg gtgacgctcg
     1681 atttcgagag tttcgagagg accctgacgg acgatgaagt aaacgattgt atcaccaaag
     1741 tgctggatgc aacatccaga gccggagtaa agttacgtgg atgacctgat actgaactca
     1801 ctgaacagga agatactggc ctttctgccg caactttgga cggatatcga tacggtcggt
     1861 ttttccgtcg caagaaagag cagtttcaag gatatagtga cctccataga catcgaaata
     1921 gaaaacagac tgcgtctctt tctcgaagaa ctcctccccg gagccgatta cctcggggaa
     1981 gagtcctcct cgtccgtcag atcggggctg atgtggatag tcgatccggt ggacggcacg
     2041 accaacttct ccaaatccaa cccgcattac tgcacgcagg tcgctctcta caaagatgaa
     2101 aagatcgtcc tggccgtcac ttacgaccac aacagaagag agctcttcca tgccatagag
     2161 gggcagggcg cctatctaaa cagcaagagg atctgggtga gcaaagtcga cgatgtaagc
     2221 gaggcttcca gccatgtggg actccagtat tcttccgagc tctctttcaa aagggtgacg
     2281 gagaggataa accgtgccat cgaagcttgc cgggccgtaa gaatcacggg cagcgcctgt
     2341 ctcgatctgg cctacgtggc ctgcggaagg gccgacatct tctgggaaga ggctctgaaa
     2401 ccgtgggacg tggccagcgg tattctcctg gtcagggagg ccggcgggga agtcaccggc
     2461 tgcctcgacg aacggttcga tatattcaaa ccggacatct tcgccacgaa cggttcgcgg
     2521 aggctggccg ggcagttcaa agaaagagtt ctttattaaa tcccgtcagg ggaagaacct
     2581 cctgtgctct acgtacatgc atttgtcctc cacataaagc acagatcttt cagaggccag
     2641 ttttctggcc ttttcgctcc ttatgtcctt ttgaagccag acggcccggg cgcccagttc
     2701 gatggcctct acggctatct cttcggcttc tgccgaaggc ctgaagatct cgaccgcttc
     2761 caggttcacg ccgtcggcta tcaccgactt caggtcggga tagaccttca tgccttcgta
     2821 ctcctccatc gacggattga ccgggattat ctcgtagccc ttctctttca gatacatcgg
     2881 aacgctgttg gagggtttgt cgggattctt ggaaaaaccg acaaccacga tgcgtttgaa
     2941 agtcgataga atctctttcg ctttgtcttc atgtttcaca ggaaaaacac ctcccagaat
     3001 gatgagagcg ctgcccagaa cgagcgagaa gaagaagaac cagtttccaa acctggtgta
     3061 aggcgtgatc tgagtgcccg gaaggtactg gaaggcacct atcacgtatt gagtgttcgt
     3121 ctcgttcatc gtcggcagtc ttttgagtac ccttccgtac ggatcaatta tggccgtaat
     3181 acccgtattg gatacctgga gaacgtacct tctgttctct atggcgcggg ctatgccctt
     3241 ggaaaaatgt tgttgcagcg cagtgtcgat gttgaaccag ccgtcgtttg tggatattac
     3301 gatgatccct gcgccgttga gcgtcaggtt ccttgccacc tcgccgtaaa gcgaatcgaa
     3361 gcagatctgg gcgccgatcc tgtggccgtc gacggagaag acggtgaact cctcgccggc
     3421 gtagaagtaa tctagaaact tcaaaaattc gaatactccc aggatcttag gccagggtag
     3481 aaactccacg aagggcgtga gcttgacctt ggcgtagaac tcgccggaaa aaccatctcc
     3541 attcacaaac ctcacctggt tgtagttctc gccgttgtaa attggaaagc cgaccagtat
     3601 ctcgagattt ctctcccgcg cgaggttgac cagccgggtt cccgtgggcg aaatccttat
     3661 atcgctcatg aaagtggcct cgggcatgat gacggtcgag ccttcgggta tctcctcgag
     3721 acccattaga acgactttca aagtctcttc gggcggagcg tagtatttca gctgttgaga
     3781 gatattggtc tgaagcgcgt acagggtttt ctcgctgctt ttgtcgaacg gccggagagg
     3841 aatcgcgctc tggataagag cgttgaccag cgctaccatc gcgacggccg ttattagcag
     3901 cggtacgcgg ccgctttttc gatcggagca gagatgggcc agataatgat tgaccaggac
     3961 aactacgaag accagaagca acggcccacc cagcgccagc agttgagaga tgcccagctg
     4021 gctgttcaag agcgcgtcag agagtctgcc gttggtgaat cccaggggcc ccagctctct
     4081 caggccctcg aagaccgtga aaaaagaagc cgcgaaaagc gctccggcga ggggacgggc
     4141 cttgaagaac ctctggaata tggagtaaac gaaaccgaaa ccggcgaaag gcacggcgat
     4201 gaccagacac atgagcacat aagcgacgac gcctatgaaa ttcgggaagg aatccagaac
     4261 ctcgggcacg ttgagggcaa gaacgggcaa ctcccagtag tgagtgatca tcaaataagc
     4321 gaaaccgaaa aggaagccct tcagggcgcc tccgaggggc cgggccgaac gcattcccat
     4381 gaagaagggg atcagcgcga tccagaccag atagccccag accattccgg gcatcgaaag
     4441 ggccgtgagg agggcagata acagaatcag tttcaaacta atccctccgc cagttgagaa
     4501 tacgatctat ggcctcggca accccgcttt catcgttggt cttttcggtc acgatcttcg
     4561 cgtgttcttt gacctggtcg ggagcgttgg ccataaccac cggcagggga cacctcttca
     4621 gaatgtcgag atcgttgtaa ttgtcgccta tgtaagccac ctcctcccgg tcgattttca
     4681 gatgttccaa tatatgttgc agaccgttgc tcttgcccac gcccggtccg aagaactcgt
     4741 agaatgcgtg gctttccaga gcgtcgttct tcaccgcagt caccctgtcg ggaccgggac
     4801 gcatggcttc gatcatttct attatcctct cttcttcgcc ctctatcgct atttcggcta
     4861 tccccggttc ccgtatcctt ttcgaagggt cctcttcgag aacgatcctg taggcgttgt
     4921 gcgcataata acccctgtaa gggctatcga agaggccggc cgtggaaatg tccggcagtt
     4981 tgaaaaagtc gaaaaagatc aggctgttca accctctctc gcgactgcgg ttgtaaatct
     5041 ccacggcctc ggccgtacct agcagacact gagagattat cctcatggct ccatcgtagc
     5101 cttcgatcac cagcgcgccg ttctggtagg atacgggaac gcttatgccc agctgctcga
     5161 cgtacttgtg tccggacacg taactccttc cggtggaaat cgacacatgg acaccgcgcc
     5221 ccagggcttc cattatcgct tcttcgtttt ctctcgatat cgacttgtcg ctgttgagaa
     5281 gagtaccgtc gagatcgatt actatcagtc tgatcataat tcacaacctc caagaatcga
     5341 ctacctgaaa agtctccagt ctgcggttgg tggatcgtgt ccattcgaaa cttacgggga
     5401 aggcgaactc cggagccagc ctgccgcgct ctatccagta acgcgcttct tcgctgttgt
     5461 agatgccggg atttgccagg attttgagtt ttgtttcacc gtctatccat ctgacctcgt
     5521 ctatttcctc cacacgaccg agcattgcgg tgattctctc tctaagctcc accggtggaa
     5581 gcggagagac attgacgagg gctatattct tcgaaagcgg tccggctatc ttcacgttcg
     5641 ccagagtgtg ggggaaggac tccagacagt ccagcaggat ctcgtttgag atagccacgg
     5701 catagccgtg ataagaggga ttggccatga tcttgaggag gtgtttcagt ttgcatcctt
     5761 cacgcaccgg ggcctcgcct attctcggaa catggatctt gagttcttcg agcgccattc
     5821 ctatcagctt tttgtcccag ccccttatag gagcatagaa accatctttc aacgcgatgc
     5881 cggtctgtcc ccacgtatct gactggttgg cgccggtggc gattattccg agggcgctgt
     5941 tgcgcatcga ggaaagtttc gcgtgtctcg tgcaccggtt gcacgaagga ccgtttttcc
     6001 agacagcctc ctgcgcctcg tatccgggtg tgaattccag tctcaaacca agcctgtcgg
     6061 ccagttcgac gacgatttct aggcctctgc tgtaactgta agggccgaag cagacattga
     6121 ccagtttcac acgatccctt ccaagcgctt cgcttgccag ggccgcaacc agcgtgctgt
     6181 cctgacctcc ggaaaaagcg accgttattc tctctcctct ggaaatctct ctgatctccg
     6241 caatagtcgt atctatcaaa ttaccttcca tatcacgcac caggtatacc ggtcccgaac
     6301 tcgacgaaac cggacgctct ctaacagata ttctagcaga cacccggcac atggttggta
     6361 agtgtgggtt aaggaagagc ggaagagccg tccgtcgtcc accgtaccgg agcatggacc
     6421 cgtccttcga aagagcggga tcttgatctt cttacctcta acctcttacc tcttacctct
     6481 ggtctgttac gtttccgtca ccagacgaaa cccctgtcga acttggattt cacgattcgc
     6541 agtttccgcg ggatgtcaag cccgtaagga catctctggc tgcagatgcc gcaatcggta
     6601 cagctgtcga tgcccggaga gagtacttcg tactcccttc tgccctgttc ttcgctcgag
     6661 aaccagtggg ccagcctgtc tctaagggcg tagtcgggag ccttcccgat cctgccgtcc
     6721 agcatctgtc tgtcgtacct gccttcggcc agaaagaagc ccggtatgtt gatgccctcg
     6781 ggacagggga ggcatttcat gcactggcgg cagacgtagt cggccagctc cggagccctg
     6841 gaaaaaagtc cgtccagttc gaagggtgtc atggaaggcc cgtcgaggac cgacaggtct
     6901 tcatctagat gctcaggcga gttcatgccg ctcactatac aggaaacggg cagtgttttg
     6961 acgtacctga aggcctcttc cacgcttctc cagagatagc cgtcggccag aggtttcatg
     7021 gccagaatac cgatcccgtt cgagagggcg tacggcagga tcttcatctc tatctcggga
     7081 aagttgaatc tgtcgaagta attgaaatga gtcatgaaga ggtcgaaggg atactcattc
     7141 aaagcccgga agagagtgcc tccgtagccg tggctcgtga tgccggcgaa ccttatgagc
     7201 ccctcggtct tcgcccattc gagagttttc aaagctccgt cggaagagat tatcttctcc
     7261 caatcgccgt tttcggtcac cgcgtggaga aagagtatat cgatatggtc tcttcccaga
     7321 gctttcaggc tttccatcag ctgcctctgc gaaccttcga aggtccgctc gcccgttttg
     7381 gtggcgacga taacgccttc tttgggaaga aagctcgaga gttttctttc agaatcgccg
     7441 tcgccgtaag atctggccgt ttcgaagtag tttcctccgg cgtctagata ttttttcact
     7501 atctttccga cggtctcgac accgatctca agaagatgaa agccgcccag gccgagcagg
     7561 gaaagcttaa ggccggtttt tccaagagtc ctgtactcca caacactacc ccctcagatt
     7621 atcttctctt ctcgagcagc tctattatcg tgttgtctat ctccgacatt cccctgctca
     7681 tgagaagctg tatattttcg atggacttgt ccagcgtcgg cgctatgaag ccctgggccg
     7741 aatcgatccc gagatcgtcg atcgccagca gggcgcaact gtaagcctct tgacctccca
     7801 gtccggcctt cagcgcgcag ctttccttag ctccgtcgca catcataccc gtcgtgttgg
     7861 aaagaaccgt gatcatggcc ctctccatca ccgatctatc gcctcccaga agataaacga
     7921 tcccggccga tgccccggtg ccggccgccg taacgcagcc gcatatgggt gctgtccttc
     7981 ccagcttctt cttgaggtaa cttgaaaaga ggtggctgat ggcgaccgcc ctggctatct
     8041 cgcccctgga tttatcgtaa tgcatgccca cgagaactac cggcagggtg gcgactattc
     8101 cctggttgcc gctgccgccg ctgctcatca ccggcagggg agccccggtc atccgcgcgg
     8161 ccgaagccgc gagacagtac ttgcggatcc tgtatcccag gcttccgctt tcgcacgcct
     8221 ctgccttctc tctgggggca actatctttt ccagaccgta tccggctatt tcgaggttca
     8281 tgttgatacc ctcgaagatt ctgtcgatct caccgtcggt catctcatcg accgccttga
     8341 agatatcttc gacggtgaag tcgatgtcgc ggctttcgac ggctccctca tcgctctcga
     8401 tctccctgcc gtccctttcg acgagcacga cccttgtgtg gtggtccttt atcacgactc
     8461 tggccgtgtg ggtgaaaccc tttacaagcg cctctatgta tattcctgtt ctcgaagtgt
     8521 cgcatctgac cgtcactctt cccgagtcga tcatctctct ggccagggcc accgactctc
     8581 cgcagcaggg tttcagcgct tccagctgga gcgacgaatc accacaaata actcccaggg
     8641 ccgcagctac ctcgtttccc ttctcgccgg ttccgggtat gtagacgtac atcccgttct
     8701 tgtaaacgct cttgctcgtt atcacttcga ctctctcaac tgaatcctgt aaatgaccga
     8761 ccgcaagcga aacacagtaa gctattgcac cgggctcggt gcaaccgagt gccggcttca
     8821 cctcttttac aagatactcc tcgagaaaat gcatttcgat cctccaatag gtaagtctct
     8881 gaaagagtat agcatcgagt gctgtaatac acctgaacga ttggagagcc ggcgcaaagt
     8941 ttggcgcctt gaaacgcttt cattccggaa atcactttca gtctcttcaa ccgtaggggc
     9001 gacaccgatc acaacggtgt ccacataaat ttacaaaacc ataattttcg tttatcgtgg
     9061 gaggagtatg atttttacca accggtaagt aaaaatcttc tgggggtgtt tttaatgggc
     9121 attgtagaaa gtatgaaagc ttcaatgttg agacaggcgg gaaagttcgt gggttctctc
     9181 gtcaagaact cttcgacgga aaatatagct cgtctcttcg gaacagtagc cacgctcagt
     9241 aaagaaccga ccaagagcgg cttgaagaaa ctcacgcaga tggccaagga cgatcatccg
     9301 atgatcaagt cctggcaaaa ggttttccag aacgcaagtc cgaaagccgt ggaaaaggct
     9361 atgaccaatc tggtggtcaa cgaattcgcc ctcggcgaaa agatccgtca ggaaaagatg
     9421 ctcgagcacg aagtcgttat acccaaactg ctcgttttga gtcccactta tgcctgcaat
     9481 ctaaactgtg taggctgtta cgccggtctc tacggaagga aataccagct gtcgaaggaa
     9541 gaggtcagct ccatcatcag gcaggcgaac gaactgggca tctacttctt cattgtaacg
     9601 ggcggagagc ctttcgtctg gccgcatttg ctggagatct tcgaggagtt caacgactct
     9661 tacttccagg tctataccaa cggtacgttg atcaccaaag aagtcgccaa aaagctggcc
     9721 gagctcggga acgccacctt cgcggtatcg gtggaaggtt tcgagggcga aaccgactgg
     9781 agaaggggaa gaggcgtctt cgaaaagatc aagacggcct gggccaattt gaaggaagcc
     9841 ggagtgattt acggaacctc cgtgacggcg acacgtctga accacgacgt catgatgaag
     9901 gacgagttct ggcagttcct caaggacaac ggcgtcgcgt acgcctgggt cttccagttc
     9961 atgccggtcg gcatgaatcc gtccatggac ctggttccga ctcccgaaca gaggtacgaa
    10021 cgtttcttca agaccgaaga acagagactc ggcggagagt tcgccttcgt cgccgatttc
    10081 tggaatcacg gattcctgac caacggctgt ctggccgccg gatcgaaata ccttcacatc
    10141 aacgccaagg gctatgtgga accgtgcgtc ttccagcagt tcgcggtgga cagcataaga
    10201 gagaagagcc tgatagagat actcaagtct cccttcttca ccgcttacaa gagaatggtc
    10261 ccttacagca acaacctgtt cagaccctgt ccgataatcg acaacccgaa agtgcttcgc
    10321 gccatggtca acaaattcaa cgccatcccg cagcacgacg ggagcgaaaa cgtggtgacg
    10381 gaactggcgg gtcaactcga tgaactggcc gaaggctgga agcagtacgc cgacaaactg
    10441 tattacgaag agggcttcgc cgaaacccat ccggctcaca ggggcgtgta cgacttcgaa
    10501 gtgagaatga ggaagtacgc caacaacgaa gaaaagcttg ctatcgataa aaaaccctga
    10561 tggatgaagg tggtgaagtc cggtgcgcgc caggttgtct tcaaaggaga gaatcgtcag
    10621 agccgcccgg aaggccttcg cagatagagg tcgggatgga gtgagcatgt cggaaatagc
    10681 cggtatcgcc ggagtaaaaa aggccctcat ctactattat tttccaagca aagaggatct
    10741 tttctacgaa gtctggcggt actcgatcga tgaactcgaa gaccacattt tttctgaagc
    10801 cgacggagaa aatgtctatc tctcgaagat aaagaagatc ctccggtcgt acatagactt
    10861 cataacaagc aagaacgaag tcaagaagat aatggaccag gagagagcca atctgaccag
    10921 agaggacata gaatcctgga aaagggtacg ggacaggtac ctgtcgctga aaaacagatt
    10981 atccacagtg atcaacgacg cgaaaacggc cagagagatt tcgaaagacg tcgatcccga
    11041 cagtacggcg gacctcatct tgaacggttt cagcatcacc gaagaccccg agggactcga
    11101 aagcataaga gagataatat ggagaggtct aataagtaat tcaggagagt caacgacccc
    11161 cggctaaagc cgggagctag tagctcaggt tgactagcct aagtgtttcg gacact
//
LOCUS       DAOOLY010000052        11172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6572_length_11172_cov_4.428173, whole genome shotgun sequence.
ACCESSION   DAOOLY010000052 DAOOLY010000000
VERSION     DAOOLY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_11172_cov_4.428173"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLD15_04140"
     CDS             complement(<1..657)
                     /locus_tag="PLD15_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB63124.1"
                     /translation="MVEISSIEKTFESRKKKVKAVDRVSFKAQPGRIYGLLGPNGAGK
                     TTTLRIIATLLKPDSGKAQVMGFDSVKDATQVRKRIGFLTSDMKLSGNLTPRELLQFF
                     GELNHMQNGDIKKAIMEMAEYLDMGEFLDKQVGKLSSGMKQKAAMAVSLIHNPEVIIF
                     DEPTNGLDILTARTVTGFLKDYRRQGKTIIISTHIMAVAEKLCDNVGIIFKGRLVEDD
                     S"
     gene            870..2300
                     /locus_tag="PLD15_04145"
     CDS             870..2300
                     /locus_tag="PLD15_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB63125.1"
                     /translation="MKVGKSRFLLDKRPALKKLVGLLGETYPYVSILGTDVAGKTYRV
                     TTTGIAVKGSDWLERGFVIRVHDGQRYFEYSFNEVDEDSLEGIAEYVRERTGSASKTI
                     KELSLSYTSLTPPEEEKSEIDFEGEVAVLPGQTSPGEIIRKLSAIRDRAHAISEEIVN
                     VMTLMENVRVSKLFVSTRKDLYQSYMWSQAALYVLARRDGITKYGIEGFSGLKGLEIL
                     DEMESKIDKVVDNARMLLSAGRIEPGVYDVICSPDVAGLIAHEAFGHGVEMDMFVKGR
                     AKAVEYMDREVASGLVTMHDGAASAREVSSYVFDDEGTLAHDTVIINGGILKRGISDI
                     ISATVLGTEPTGNGKRESFERKAYARMTNTYFAPGSDSLEEMIASIESGYLLENYYSG
                     MEDPKNWGIQCVIAYGREIKDGKFTGKVVSPVMMTGYVPTLLKSISMVSGEIELSGTG
                     ACGKGHKEFAKVSSGGPYIKAKARLG"
     gene            2300..3574
                     /locus_tag="PLD15_04150"
     CDS             2300..3574
                     /locus_tag="PLD15_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPB63126.1"
                     /translation="MLEKIVKRLQKADINGWKIEEKFIEGQEYFFVKRELDLSRAKKV
                     RHFDLTVYRDFYESGEKYRGSSSTRLSPTMSDDELQAAIDEAAFAAGFVKNPWYPLVS
                     SSVERLGSHDRDLQELSSNAMEAIFKADNREGAWLNSMELFITRSSHRIINSEGVDVS
                     YTGYRTYIETIVNASGKEEVELYDQLLLSLPEKRIIEERIHDLLAKAIDRSLAVSTPF
                     LKAIPVILTGDPAKEVARYYLNQSSARMKYDRISQVEPGESVQGDERGDRINLSLVPD
                     LEESYFSAPFDEDGFKLSRRDIVKYGLLAGYWGNLWYSSYLGVETTGSAMNFSLLPGS
                     VGIEEMRSRPHLEVPNFSAVEVDEATGDFGGEIRLGWYFDGTKRVPVTGGSITGNLRD
                     IESLYLSREVELESDYLGPKSMMIEGFNIAGE"
     gene            3716..4381
                     /locus_tag="PLD15_04155"
     CDS             3716..4381
                     /locus_tag="PLD15_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB63127.1"
                     /translation="MIIETIRMRHSTRKFVPYSLSEDEKKHLKEFMAGVSSLHSRHLE
                     WILADHNRGSGVIYAPCPDKSSCLVEYGFQGEQIVLELTKMNLASCWNGSIKNDGAPA
                     GIVVGKEETGKLSLNDLLTGMGKRKELPALVEGPVPQDEKLLQILEACRLAPSAMNRQ
                     PWKFSVQKDGLYIWTRSDLLGNHHWLDLGIVLTHAFLVAKEFFKNVSIEKAAGDKYRL
                     VMR"
     gene            complement(4391..6160)
                     /locus_tag="PLD15_04160"
     CDS             complement(4391..6160)
                     /locus_tag="PLD15_04160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB63128.1"
                     /translation="MKVRFSWFRVSVFLICVFLAGVFIFVIPSEKPARLLTVLSESVL
                     VGASLVVAFVLKRSLVHPGIPAGLLLISLSVYMDLLQSITGLILFETLAVVLSIIAAI
                     VLLYYIVLHVRNLRIMIIEHETIINGSNEGVILRNLKDNTVRFNEAARKILGYEPEEL
                     KRLSIYDIVLPQELEFHGEMMERVMKGETISPYEARYAGKNGKEIDVQVNASLVKNDK
                     GIPDYIIFAFRDITRIKAMELSLRELNRFHRALNEVVTEALESGLDNNAYHDFLFRCV
                     EAFPGADAGVFLLKEEDERFHYVAACNYDFEELKKVSFATDELIQANSDRVTVIKDYA
                     VDYEMDDERQRIILTAGRLGEIKSTITIPILIDGKVMMYFNLDSLKSSTVFDNPEIQE
                     IAASFGRAVAVLLSRLRLERELNKQRELMERLSMEDPLTGLPNRRYFFDCSVRQIEYL
                     RRRKEEMTILYLDLNDFKGVNDTMGHDFGDELLKEVGKRLNSICRSSDLLARMGGDEF
                     VYVLPSTGKEEIAEVIKRIRNSFKEPFVVENKSIYLSTSIGKALFPEDGQSVRELLIV
                     ADERMYSDKDRTNGRINKSRIEE"
     gene            complement(6201..6875)
                     /locus_tag="PLD15_04165"
     CDS             complement(6201..6875)
                     /locus_tag="PLD15_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HPB63129.1"
                     /translation="MKRSFILVLLLVSTFTVLFSIELKDSGNQMKATQLAVLEIDETP
                     ATGYLWHIGAEPSNVLRNFLEEHMAGSAFPGAPSVKGWIMTAAKEGKALLTLKLYRDW
                     EGERKAIDFRAVTVDVSGEEKGQVDLKIVLNEMALGSTATVTLEENGSTGYTWRYHIL
                     GEGLQEIKKETIADTSGLVGAPAKVIWTFQAVDEGYTTIVFRYFRSWEGEGSAVDFRA
                     FNVFVE"
     gene            complement(7118..7327)
                     /locus_tag="PLD15_04170"
     CDS             complement(7118..7327)
                     /locus_tag="PLD15_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63130.1"
                     /translation="MESNGYIDVDGITYKLVSRQKSRHKQIKIRTRGPMGVRLEREES
                     FFKSNREYLKTLVSDSEVNIPLEKE"
     gene            7452..8252
                     /locus_tag="PLD15_04175"
     CDS             7452..8252
                     /locus_tag="PLD15_04175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63131.1"
                     /translation="MLVELRSEDFSRVRPLLGEISYNLAVPSILEGNTLGKLFVCNEE
                     KPDSVIVWNCHDTVLIGGDPGEESKGEYGEFLEKYLIPAAGEKGIPVLNFYSTGKAKR
                     CLRDLLGDLEPKPILRYLFRLNPSGFGPSTLESDSAVRITAELLESSLENIDHLTGWI
                     LSFWPNLDSFLENSLGFCAVEDSKIVSLCIGVYRSKNHIELGAETLKGFKRLGFGRKV
                     ALACVKESCSKGLIVDWQCEASNNPSVLIAKRLGFQEVLQYTISQISI"
     gene            8270..9445
                     /locus_tag="PLD15_04180"
     CDS             8270..9445
                     /locus_tag="PLD15_04180"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB63132.1"
                     /translation="MYRLICTGCGKEYPGDSLAFRCGCGEPLEVLFAPESKSFSSEGQ
                     SILNRFSSFLPPLKDEAFLSMGEGWTPMSRSFAFASECDFEFYLKNESINPTWSFKDR
                     GTYLTLLDAVEKGYKGFGTVSTGNMAASVAAYGARMGMNTTILVSKNIPAEKIRPIAI
                     YGPRVIKVDGDYGELYLKSLETGPSKGIYFSNSDVPMRVEGSKTIAFEIYLQLGEKVP
                     DFVIVPTSSGGNIRGIEKGFREIAEAGLSERTPVMVAVQACGCAPIHNAFDSGREKIE
                     RVPNPSTIAHAIENPFPPSGNAVLQMLKKNGGLTLCLKEEEILEAQTRLAGEGLFAQP
                     AGVTSVAAIKKLKELIPLKKARIVSIITGSGLKYPSILERHCLSAESISIGDLEAAL"
     gene            9809..10804
                     /locus_tag="PLD15_04185"
     CDS             9809..10804
                     /locus_tag="PLD15_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB63133.1"
                     /translation="MKPITIEDVARLSGVSRGTVSRVINGSQNVNSETRKRVLEVIEK
                     LGYRPNQNARRTAGGRSSTIGIVIPMIGTEFYERLIKSMEEKLRAQWYAMMLFPVLKR
                     DWLSKVMMHDIPNSEAAGMFLCSFDIEHLFSSFRKPFEGPTVLVDSYSPLYDSIYIDN
                     ELGGYLVGRKFSQLEGSTHVIMMQESIDSGFWPVFNSRFKGFQKALLESGKSLPQRNV
                     HSYDFSWSAGALAARDIFDNDRPPFNIFAMSDLYAFGVMREAQLRGLRIGRDVKLVGY
                     DDQSLARDWGITTVRQPIEQMGEKAVNMMLQRLERPGKEVESLCLKPEMVERESA"
     gene            10842..>11172
                     /locus_tag="PLD15_04190"
     CDS             10842..>11172
                     /locus_tag="PLD15_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB63134.1"
                     /translation="MRRKVLIVLAVSMLFLGSVITATVDGQLIIWADDTRTPIFKELA
                     REYTEMTGITVTVYEMTMDAITEQVVTASPAGEGPDLIVGAHDWIGRLVRNGTIAAID
                     LPEDLLLQ"
BASE COUNT         2830 a         2759 c         2858 g         2725 t
ORIGIN      
        1 cgagtcatcc tcgaccaggc ggcccttgaa tattatgccg acgttgtcac agagcttttc
       61 ggcgacagcc attatatggg tcgatattat tatcgtcttc ccctgtctgc gataatcctt
      121 caagaacccc gttaccgttc ttgcggtcag tatgtccaga ccgttcgtag gttcgtcgaa
      181 gattataact tcggggttgt gaatcaggct cacggccatc gcggcctttt gtttcatacc
      241 gcttgagagc tttccgacct gtttgtctag aaattccccc atatccagat attcggccat
      301 ttccatgatg gccttcttta tatcgccgtt ctgcatatgg tttagctctc cgaagaactg
      361 gagcaactct ctgggcgtaa ggtttccgga aagtttcata tcgctggtaa ggaaaccgat
      421 cctcttgcgc acctgggtcg catctttgac cgagtcgaac cccattacct gcgctttgcc
      481 cgaatccggc ttcaaaagag tggcgatgat gcgcaaagta gtcgtcttgc ccgccccgtt
      541 ggggccgagc aagccgtata ttctgcccgg ttgcgctttg aagctcactc tgtcgacggc
      601 tttcactttt ttctttctgc tttcgaaggt cttctctatc gaactgatct cgaccatcct
      661 gcacctcctt gctgtcgaaa taacccgaag aacatcgtct ccattcgata aaaaacactg
      721 ttttagtttg acttcaatta ctttctatat tatgagccgt atagtaaata atatcaagtg
      781 gcattttcag ttatttcatt tattaattaa ctatagtata atggtcttca gaacagggca
      841 agttacaacg aaaaatcagg aggtaaatga tgaaagttgg aaagtctcgg tttttactcg
      901 ataaaaggcc cgctctgaag aagctggtag ggcttctagg agagacgtat ccatacgtct
      961 ccattctcgg aaccgatgtg gcgggcaaga cctacagggt cactacaacc ggaatcgccg
     1021 tgaagggaag cgactggttg gaaagaggct tcgtgatcag ggtgcatgac gggcagagat
     1081 acttcgagta ttcattcaac gaagtagatg aagactccct agagggaatc gccgagtacg
     1141 taagggaaag aactgggagc gcttcgaaga ccataaagga gctgtcgctc tcttacactt
     1201 ctctgacacc gcctgaggag gagaaaagcg agatcgattt cgaaggcgaa gtggcggtat
     1261 tgcccggcca gacgagtccg ggcgagataa tccggaagct ttcagccata agggacagag
     1321 cccatgcgat ttccgaagag atagtgaacg tgatgacgct tatggagaac gtgagggtca
     1381 gcaagctctt cgtatccacc aggaaagacc tttatcagtc gtacatgtgg tctcaggcgg
     1441 ctctttacgt tttggccagg cgcgacggaa tcaccaaata cggtatcgag ggcttttcgg
     1501 gcctcaaagg actggaaata ctcgacgaaa tggaaagcaa gatcgacaaa gtcgttgaca
     1561 acgcgcgcat gttgctatcg gctgggcgaa tcgagcccgg tgtttacgat gtaatctgtt
     1621 ctcccgatgt ggccggactg atagctcacg aggccttcgg ccacggcgtg gaaatggata
     1681 tgttcgtcaa gggaagggcg aaagccgtgg aatacatgga cagagaagtc gcctccgggc
     1741 ttgtcacgat gcacgacggg gccgcttcgg cgagagaagt ctcatcgtac gttttcgacg
     1801 atgaaggaac tctggcccac gacactgtga tcatcaacgg gggtattttg aagcgaggta
     1861 tatccgatat catatcggcg acggttctgg gcaccgaacc caccggaaat ggaaagaggg
     1921 agtctttcga acgcaaagcg tacgccagaa tgacgaacac ttatttcgcc ccgggttccg
     1981 actctctgga agagatgatc gcctcaatag agtccggata tctcctggag aactactaca
     2041 gcggtatgga agatccgaaa aattggggaa tccagtgtgt gatcgcctac gggagagaga
     2101 taaaggatgg aaaattcacc ggaaaggtcg tgtctcccgt gatgatgacc ggttatgttc
     2161 ccaccctgtt gaaatcgatc tccatggtct ccggagaaat cgagctttcc ggaaccggtg
     2221 cctgcggcaa gggtcacaaa gaattcgcaa aggtttcctc cggaggacca tacataaaag
     2281 ccaaagcgag gttgggataa tgcttgaaaa gatagtcaaa cgtctccaga aagccgatat
     2341 aaacggctgg aaaatagaag agaaattcat cgaaggtcag gaatatttct tcgtcaagag
     2401 agaactggat ctgagtcggg cgaagaaagt gaggcatttc gacctcaccg tttacagaga
     2461 tttctacgaa tccggagaga agtacagggg cagttccagc accagattgt cgcccacgat
     2521 gtcggatgac gaactacagg ccgcgataga cgaggctgcc tttgcggccg gtttcgtcaa
     2581 gaacccgtgg tatcctctcg tttcttcttc agtggagcga ctgggatcgc atgatcgcga
     2641 tcttcaggag ctctcatcga acgctatgga ggcgatcttc aaggccgata accgcgaagg
     2701 ggcctggctc aactcgatgg agctgttcat aaccaggagc agtcacagga taatcaactc
     2761 cgaaggtgtc gatgtatcct acaccggata tagaacatat atcgaaacta tcgtgaacgc
     2821 ctccggaaag gaagaggtcg agctgtacga ccagctgttg ctatcgctgc cagaaaagag
     2881 aatcatagag gaaagaatcc atgatctcct ggccaaggcc atcgatagat ctctggccgt
     2941 atcgactccc ttcctgaagg cgataccggt tatactcacc ggcgacccgg ccaaagaagt
     3001 cgcgcgatat tatctcaacc agtcgagcgc caggatgaag tacgatagga tctcgcaggt
     3061 tgaacccgga gagtcggtac agggagatga gaggggcgac agaataaacc tgagcctcgt
     3121 tccagacctt gaagaatctt acttttcagc ccccttcgac gaggatggtt tcaaactgtc
     3181 gagaagagat atagtcaagt acggcctgct cgcggggtac tggggcaact tgtggtactc
     3241 cagctatctc ggtgtcgaaa ccaccggatc ggcaatgaac ttttccctgc tcccgggcag
     3301 cgtgggcatc gaagagatga ggtcccgccc gcacctggaa gttcccaatt tttccgccgt
     3361 cgaagtggac gaggcgaccg gtgatttcgg aggagagata agactgggct ggtatttcga
     3421 tgggaccaaa agagtgccgg tgaccggcgg gtcgatcacg ggcaatctga gagacatcga
     3481 atctctttat ctatcgcgcg aagtcgaact ggaaagcgat tacctgggtc cgaagtccat
     3541 gatgatagag ggcttcaata tagccggtga ataatcgctc gaacaaaagt cccggtagct
     3601 tcaatctaat atacgcatcg ggaatttgca gcgaaccgtt gaagtctctg aatgtcgtta
     3661 aaatttacag aaattctccc ggccggggcc tggttaagca aaaggaggag gagtaatgat
     3721 aatcgagaca ataaggatga gacactcaac aagaaaattc gtaccttaca gcctttccga
     3781 agatgagaaa aaacatctca aggaattcat ggctggtgtt tcttccctcc actcccgcca
     3841 tctggagtgg attctcgctg atcacaacag gggttcgggg gtcatatacg ctccctgccc
     3901 cgacaaatcc tcctgtcttg tggagtacgg ttttcagggc gagcagatag tgctggaact
     3961 caccaaaatg aatctcgcca gttgctggaa cggaagtata aagaacgacg gcgctccggc
     4021 cgggatagtt gtgggtaaag aagagacggg caaactttct ctgaacgatc tgttgaccgg
     4081 catgggaaag agaaaggagc taccggctct ggttgaaggg cctgtccccc aggacgagaa
     4141 actactgcag atactcgaag cctgcaggct cgcgccctcg gccatgaaca ggcaaccctg
     4201 gaaattcagc gtgcagaagg acggcttgta catctggacc aggtccgacc ttctcggaaa
     4261 tcatcactgg ctggatctgg ggatagttct cacccacgcc tttctcgtgg cgaaagaatt
     4321 cttcaaaaac gtatcgatag agaaagcggc aggagataaa tacaggcttg ttatgcgctg
     4381 acgggggaga ttattcctcg attctggatt tatttatcct tccgttcgtt cggtccttgt
     4441 cggagtacat tctttcgtcg gctactatca gcagttcacg tacactctga ccgtcctcgg
     4501 gaaagagcgc ttttcctatg ctcgtcgaaa gatagatgct tttgttctca acgacgaaag
     4561 gctctttgaa ggagttcctt atcctcttta taacttcggc gatctcttct ttcccggtcg
     4621 aggggagaac gtaaacgaac tcgtcgccgc ccattctggc tagtagatcg ctgctccggc
     4681 atattgaatt caatctcttt ccgacttcct tcaacaattc atctccaaaa tcatgtccca
     4741 tcgtatcgtt gacacctttg aagtcgttga gatccaggta gagaatcgtc atttcttctt
     4801 tccttctgcg gaggtattcg atctgtctga ccgagcagtc gaagaaatac cgtctgttgg
     4861 gaaggccggt gagcggatcc tccatagaga gcctttccat aagttcccgc tgcttgttca
     4921 gctctctctc cagccggagc ctcgaaagga gaacggccac cgccctgccg aagctggcgg
     4981 cgatttcctg gatttcggga ttgtcgaaaa cggtcgagga ttttagactg tcgaggttga
     5041 agtacatcat gaccttaccg tcgatgagta tgggaatggt gatggtactc ttgatttctc
     5101 ctagccgacc ggccgtaagg attattctct gacgctcgtc gtccatctcg tagtcaacag
     5161 cgtaatcctt tatcaccgtt accctgtcgc tgttggcctg gatcagttca tcggtggcga
     5221 aggatacctt tttgagttct tcaaaatcgt agttgcaggc cgcaacgtaa tggaacctct
     5281 catcttcctc cttcagcagg aaaacaccgg catcggcgcc gggaaaggct tcgacacacc
     5341 taaaaagaaa atcgtggtat gcgttgttat ccagaccgct ctccagtgct tccgtcacca
     5401 cttcgttcaa agccctgtgg aacctgttca gttccctcag agatagttcc atcgctttga
     5461 tcctggttat gtctctgaag gcgaatatta tataatcggg gattccctta tcgtttttga
     5521 cgagcgaggc atttacctga acgtctatct cttttccgtt ctttccggcg tacctggcct
     5581 cgtagggaga tatcgtttcg cctttcataa ctctctccat catctcaccg tgaaattcca
     5641 gctcttgagg gagcacgata tcgtaaatgg agagcctttt cagctcttcg ggctcatagc
     5701 cgagtatctt gcgggccgct tcgttgaaac gaacagtatt atctttgaga ttgcgcaaga
     5761 ttaccccttc gttggaaccg tttattatgg tctcgtgttc gattatcata atcctcagat
     5821 ttctcacatg cagcacaata tagtatagta gaacgattgc cgctataata gacaaaacaa
     5881 cggcaagcgt ctcgaagagg atgagcccgg ttatggactg tagaagatcc atatagaccg
     5941 acagggatat gagcaggagc cccgcgggaa tcccggggtg aacgagactc ctcttgagca
     6001 cgaacgccac cacaagggat gcgccgacca gaaccgactc gctgagaacg gtcaaaagcc
     6061 ttgcgggttt ctccgagggt ataacgaaaa tgaatactcc cgcaagaaag acgcatatga
     6121 gaaagactga aactcgaaac cagctgaaac gtactttcaa gataaattca ccccggattt
     6181 ccctttcttg ataacttcgc tcactcgacg aacacgttga aagccctgaa atctacagcc
     6241 gatccttccc cttcccacga tctgaagtat ctgaacacaa ttgtggtgta accttcgtca
     6301 acggcctgaa aggtccatat gactttcgcg ggagccccga ccagaccgct ggtgtctgcg
     6361 atggtctctt tcttgatttc ctgaaggccc tctccaagaa tatgatatct ccaggtgtaa
     6421 ccggtgcttc cattctcttc cagagtcacg gtcgccgtcg atccgagcgc catctcgttc
     6481 agcactatct tcagatccac ctggcctttc tcctctccgc tcacatctac agtcaccgct
     6541 ctgaaatcta tggccttcct ctctccctcc cagtctctgt agagcttgag agtaagcagc
     6601 gcctttcctt cttttgcggc cgtcattatc cagcccttga cggacggtgc acccggaaag
     6661 gccgaaccgg ccatgtgttc ttcgaggaaa ttgcggagaa cattggaagg ttccgcgcct
     6721 atgtgccaca ggtaaccggt ggcgggggtt tcatcgattt ctagaacggc cagctgcgtc
     6781 gctttcatct ggttgccaga atctttcaat tcaatcgaga acagtaccgt gaacgttgag
     6841 accagcagca aaacaaggat aaaacttctc ttcatgtagt cacctccctg aatcgaaaac
     6901 gacttcccaa tatccctata ttcgataaaa tcgatcgaaa aaccatcata ccacatcccg
     6961 agttcccgta gatctttcga tcgtattaat gctctggatt ctcttaattc actacatgaa
     7021 attccgtgcc agaaagcaca gatctcgatt cgtcattcga ccggattatt ccggctgcgt
     7081 atattcagat tcaaaagcgg ctatattcgg gaagggttca ctccttttcc agcggaatgt
     7141 tcacctccga atcgctcacg agggttttca agtattccct gttactcttg aaaaagctct
     7201 cctccctctc caatcttacc cccatcggac cgcgagttct tatcttgatt tgcttgtgcc
     7261 tgcttttctg tctgctcacg agtttgtaag ttattccatc gacgtcgata tagccgttac
     7321 tctccaaact atcacctcct cgaataattt accatttttg ggaaaaaaca ttgtcatgtc
     7381 tcattgtgtt gcattgtaaa gtgggtttga agtagaattg attaattagc ccagacgaaa
     7441 ggagattgca gatgttagtc gaactccgtt ccgaggactt ctcccgtgtc agacctttgc
     7501 tgggtgagat ttcttataat ctagccgtgc catcgatttt agaaggaaac acattgggga
     7561 agttgttcgt ttgcaacgaa gagaaacccg attcggttat tgtctggaat tgccacgaca
     7621 cggtattgat aggcggagat cccggagagg aatctaaggg agagtatggc gagtttctgg
     7681 aaaagtacct gattccggcc gccggagaaa agggtattcc tgtgctcaac ttctactcga
     7741 ccggtaaggc aaagagatgc ttgagggatc ttctaggcga tctggaaccg aaacccatac
     7801 tcaggtacct gttcagactc aacccgtctg gcttcggccc aagcaccctt gaaagcgatt
     7861 cggccgttag aatcaccgcg gagttgctgg agagcagtct ggaaaatatc gatcacctta
     7921 caggatggat cctatctttc tggccaaacc tcgatagttt ccttgagaac agtctcggct
     7981 tttgtgccgt ggaggacagc aagatcgtga gcctgtgtat aggggtttac aggtcaaaga
     8041 accatataga acttggggcc gaaacgctga aaggcttcaa acgactgggc tttggtagaa
     8101 aggttgcttt ggcctgcgtg aaagagagtt gttcgaaggg tttgatagtt gactggcagt
     8161 gtgaagcctc caacaatcca tctgtcctta tagccaagag gctgggcttt caagaggttc
     8221 tccagtatac tatcagccag ataagcatct gaacctccgg aggaagaaat tgtatcggtt
     8281 gatatgcacg ggctgtggaa aagaatatcc gggcgactct ctcgctttca ggtgcggctg
     8341 cggagagccc ctggaagttt tgtttgctcc ggaatcaaaa agcttctcct ccgaaggaca
     8401 gagtatcctg aaccgctttt cgtcgtttct tcccccgttg aaagatgagg cgttcctttc
     8461 aatgggggag ggatggacgc ccatgtcccg gtcatttgcg ttcgcttccg agtgcgactt
     8521 cgagttctat ctcaaaaacg aatccatcaa tcccacctgg tctttcaaag acaggggaac
     8581 ttatctcact ctgctggacg ccgttgaaaa gggatacaaa ggcttcggga cagtatctac
     8641 cggcaacatg gcggcttcgg tggccgccta cggcgcgcgg atgggaatga atacgacgat
     8701 cctggtgtcg aaaaacatac ccgcggagaa gatccggccc atcgccatat acggaccgag
     8761 ggtgatcaaa gtcgatggag attacggcga actctacctc aaatcactcg aaacggggcc
     8821 ttcgaagggt atatacttct ccaattcgga cgttccgatg cgagtggagg gctcgaagac
     8881 catcgccttc gagatttatc tgcagctcgg tgaaaaagtg ccggatttcg tcatcgttcc
     8941 gaccagttcg ggcggcaaca tacggggtat cgaaaagggc ttcagagaga tcgcagaggc
     9001 cggcctttcg gaaaggacgc cggtcatggt ggccgttcag gcctgcggct gcgcaccgat
     9061 acacaacgcc ttcgattctg gccgggagaa aatagagagg gttccaaatc cctccacaat
     9121 agcgcacgcc atagagaacc ccttcccgcc gagcgggaat gccgttcttc aaatgctcaa
     9181 gaaaaacggc ggcctgaccc tttgcctgaa agaagaagaa atactggagg cacagacgag
     9241 acttgccgga gagggactgt tcgctcaacc ggccggcgtg acgtcggtcg cggcgataaa
     9301 gaagttgaaa gaactgattc ctctgaaaaa ggcccggatc gtctcgataa tcaccggatc
     9361 tgggctgaag tacccctcga tacttgaaag acattgttta tcggccgaaa gcatatccat
     9421 cggagatctc gaagccgcgc tatgattgga taaacttctc cggtcctctt ccaaggacgt
     9481 cttaatattc ttgccagact ctacatccag tgtcccagac tcggcccttc ccaacgcggg
     9541 cagggaggaa tccatgcttc gaattggaaa tactctcaac ggacatcttc ttgttgtgag
     9601 cgctcacaat aacgtgatat tggcgagact gctggcaatg ccaatttata gtcttaggaa
     9661 atgtatatcg ctataattgt ttttaatggc cattaacgta agatatttcc actcaaatgt
     9721 gagaattgac ttttgttgct cgattatgat atattattgt gagccctcac aatattcaat
     9781 tcatatcttt cgacagggga gtgagattat gaaaccgata acgatagaag atgtcgccag
     9841 actcagcgga gtatcgcgag gaacggtgtc gagggtaatc aacggaagtc agaatgttaa
     9901 cagcgaaact agaaagagag tgctcgaagt aatagaaaaa ctcggttacc gcccaaacca
     9961 gaacgcccgg agaacggccg gtggaagatc ctccacaatc ggtatagtga ttcccatgat
    10021 cggtacggag ttctatgaaa gactaataaa aagcatggaa gagaagctca gagcgcaatg
    10081 gtacgccatg atgctcttcc ccgttctcaa aagagactgg ctctcgaaag tcatgatgca
    10141 cgacattccc aattcagagg cggctggaat gtttctttgc tcgttcgata tcgagcatct
    10201 cttttcatct ttccgtaaac ccttcgaagg tccaacggtt cttgttgaca gctattcgcc
    10261 cctgtacgac tcgatctaca tagacaacga gctcggagga taccttgtgg gaaggaagtt
    10321 ctcgcagcta gaaggaagca cacacgtcat aatgatgcag gaatcgatag attcgggctt
    10381 ctggcccgtc ttcaactcaa gattcaaagg ttttcagaaa gcgctcctcg aaagcgggaa
    10441 aagccttccg cagagaaacg ttcactccta cgatttcagc tggagcgccg gagctctcgc
    10501 ggcccgcgat atcttcgaca acgatcggcc tcccttcaac atcttcgcca tgagcgatct
    10561 ttacgcattc ggagttatgc gggaggccca gctcagaggc cttcgcatcg gtcgggatgt
    10621 gaagctggtc ggttacgacg atcaatcgct cgccagagac tggggaatca ccacggtgag
    10681 gcaaccgata gagcagatgg gagagaaagc cgtaaatatg atgctccagc gcctcgagag
    10741 acctggaaag gaagtcgaaa gtctctgctt gaaacctgaa atggtggagc gtgaatccgc
    10801 ttgagcctgg aataattcca gggtatagaa aggaggagaa tatgagaaga aaggttctga
    10861 tcgttcttgc ggtttcgatg ttgtttctgg gttcggtgat aactgccacg gtcgacggac
    10921 agctgataat ctgggcagat gacacgagaa ctccgatctt caaggaactg gccagagagt
    10981 acaccgaaat gaccggtata accgtgacgg tctacgaaat gaccatggat gcgattaccg
    11041 aacaagtggt tacggcctct ccggcgggcg agggtcccga tctgatcgtc ggggctcatg
    11101 attggatagg aagactggtc cgcaacggta ccatcgccgc tatcgacctt cctgaagatc
    11161 ttctcttgca gt
//
LOCUS       DAOOLY010000053        10960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6746_length_10960_cov_5.009537, whole genome shotgun sequence.
ACCESSION   DAOOLY010000053 DAOOLY010000000
VERSION     DAOOLY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6746_length_10960_cov_5.009537"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLD15_04195"
     CDS             complement(<1..462)
                     /locus_tag="PLD15_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63135.1"
                     /translation="MSKASVSVRIESVTKIFKDLRGKADVVAVNDANFEIEPGELVTL
                     LGPSGCGKTTTLRMIGGFELPTKGRIFLGNEDITFLPPNKRDTATVFQSYGLFPHMNV
                     FDNVAYGLKLRKVPSKQIKEKVMNTLGLVGLRDLAQRPPSKLSGGQQQRVAL"
     gene            complement(483..2696)
                     /locus_tag="PLD15_04200"
     CDS             complement(483..2696)
                     /locus_tag="PLD15_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB63136.1"
                     /translation="MIALYSWKKLLSFVVLSVAVFVVLLWIFNTLKQSFYKITRDNQR
                     QLVTVLSEAAPREAELAESWIVAANREFTDADIIYVDGVPGWGELTVFAPDPKLKSFF
                     DEYNGSADFEKGYESVYYLENYFSGLDYSFNDQRVSLVFSPLIDETGYDITGFLVFLM
                     SAEGGKNYARLLDIFMIGAFAVFVIVYFISKFFRDPAMGYIILGLFIMVGIFVAYPLF
                     EAVRLTFLKDGNFSMDTWVSILTSRQYLGALWGSVKLGMLTATFSTVIGFIFAFVINR
                     TAIKGKRFLSTMGLLPVISPPFSLSLSLILLFGSNGLITRQILGLKDFTIYGLGGLTA
                     VQTIGMFPIAFLTLSGVLQAIDSTLEEASLDLNGSRWKTFSRITFPLAIPGVLSSWLL
                     VFTNSLADFANPLLLAGSYRVLSVEAYIEVTGRNRLGHGAALSVLLLLPSVTAFLVQR
                     YWVSRKSYVTVTGKPSARLSDLVSRPVKYSLLAFISLFIVFILGLYGTIVAGCFVANW
                     GIDYSFTLKNITEALQRGRDAIFDTFTLSAIATPIAGLIAMMAALVIVRKKFAGKRVL
                     EGLILAPFAIPGTLIGIAYILAFDEPPLILIGTGAIIVINYIIRELPVGVEGGVATLR
                     QIDPAIEEAAQDLGADSTTVFRTIVLPLIRPAFISGMSYTFVRSMTAVSAIIFLISAQ
                     WYHMTVLIYNFSESIRFGLASVLSTVLIIIVFATFGLMRLLVRKNEYLEKSVNLN"
     gene            complement(2763..3758)
                     /locus_tag="PLD15_04205"
     CDS             complement(2763..3758)
                     /locus_tag="PLD15_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63137.1"
                     /translation="MKRFIVVIGIVCMVFSTALAAESFTVYTTLEELAAKQLFDRYEE
                     VTGIRVDWVRLSGGEAEARMEAEKMNPQASIWVGGVGLNHISAKLKGLTTPYFSRAAA
                     NTPEQYKDPEGYWIGLYLGPLAFVTNVDRARELGIEPPKGWFDILDSKYTNLVRVANP
                     STSGTAYNLITCMISLFGGDEDLAFLYLKKLDRSIDMYTRSGSAGGKSVAIGEIPVAI
                     GYAHDMVKLKAEGANVIITVPEEGTGFEIASMSLIKNGPDPVNAKKLYDWILTEEAQG
                     IIAGWYVIPVSKIAPKHPLSFSMDEIKTVHQDYVWDAENKERLLDRWTREIGAGL"
     gene            4016..4759
                     /locus_tag="PLD15_04210"
     CDS             4016..4759
                     /locus_tag="PLD15_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB63138.1"
                     /translation="MKYLQKVLNVICIVAYSDIEGVSVIEVSEKAGLPMSSTHRILND
                     LVECEALVKNMESRKYRLSPRIVPLVHEISRKMNLDWLKVILTELKDEINETVFLSEL
                     MENGVSSLLTVESNRVFSFRARSGVYLPVHCTAAGLAIVAYIEPSRAEKLMCISSSYS
                     DSQVSQCPVEEFRDRLAKVRRDGFAFCNEEYEPGLRALAVPVLRPNGFAFASITAIAP
                     GERFSDEEGNRQVVERMKEAAVKVGKFMY"
     gene            4768..5532
                     /locus_tag="PLD15_04215"
     CDS             4768..5532
                     /locus_tag="PLD15_04215"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63139.1"
                     /translation="MSEEVVRVKNVGRTFVSGGKKDRKEFTALEKVSFEVGRGEFVSL
                     LGPSGCGKSTLLKIISGLLPATSGSVEITESVNEKVPPFGFVFQDSVLLPWRTALDNA
                     LFPFEIMGNREKSDEKHVRELFALARLVGFEESYPRQLSGGMKQRVSIVRALSYNPPI
                     LLMDEPFGALDAITRDNLNNVLLEIWESTRKTVIFVTHSISEAVYLSDRIIVMGTKPG
                     RVLKDMKVTLPRPRNEDTKLSPEFYAYVRELREMLK"
     gene            5551..6333
                     /locus_tag="PLD15_04220"
     CDS             5551..6333
                     /locus_tag="PLD15_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63140.1"
                     /translation="MNKRVLITVLSILFLFLFLGIWKGYIVLNDVPPFILPPPEAVGQ
                     RFVDLIADGTFIREGWVTLYTIILGFSIGSALGFLAGYFSARSRTFEEIISPYVMVIQ
                     ATPKVSLIPLFVIWFGLGMPSKLLLIVLSAFFPVMVNTILGLRSIPEDYSNLMTVLKA
                     SEGQRVLKLELPMATPLIMAGLKIAMVQSVIGAIVSEWMAGDRGLGYLLVYGSSLYDA
                     NLLMASILATTFLGLVLYWSIELVEKKLLFWHESKLAFTEGT"
     gene            6333..7109
                     /locus_tag="PLD15_04225"
     CDS             6333..7109
                     /locus_tag="PLD15_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPB63141.1"
                     /translation="MDDKKLKSIAGNPVDDGLLPHLKVRTDLKVPELFFLPGDPARLK
                     LFRELSDEFQLLNENREFSIAVGSYSGRRFGVCSTGIGGGSSEIVMVELKELGVEKVV
                     RVGGCGALREDIECGGMVLNSGVVRLGGSSKMYVRPEYPAVADPFMLVALHESVLRAG
                     KKVCVGIGATVDSYYAGQGRFLPAIEGLGERDLLERMRAAGVVDFDMESETIFTLASL
                     MGMRAANILAVHGNRASNMWLSDYRSAQIDAIKIALEAEL"
     gene            7135..8100
                     /locus_tag="PLD15_04230"
     CDS             7135..8100
                     /locus_tag="PLD15_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63142.1"
                     /translation="MKKAAVILLVLFISLFAFAQKVTIQIEGSAVPYYIPIYMGIELG
                     YFKEEGLEVEYFFGNASDIIRNVAVGNVEFGFPNGEPVIVARSQGIPVNVIHTTYQHG
                     LGSTIFLKDSGIQTPTDLKGKKVAITSYGSSNYVQIQVLLKKNGMTLNDIQLEIIGTE
                     AIVPALVNKRVDAICFSMLRTFELRYQGIEVEEFRSDEFLPSFGNVLVTGDKILKDNK
                     DLAVKFVSALNKVMTYLSDTENMLKATYTAIEKYTPTYAGREEYMAAILSDIYAGYLW
                     QSEDTEAFGFGYGNVQRWQDSIDIMKEYQIITSDVKASDFIVTGL"
     gene            8129..8896
                     /locus_tag="PLD15_04235"
     CDS             8129..8896
                     /locus_tag="PLD15_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63143.1"
                     /translation="MKKKLGIKLMSFLVLVAIVVVWKVMVTLLKTPTHILPPPEDILF
                     KFIELIRNSVLQKNFLATFEEIVIGFSAGAGLGIVIGYILAKVELLERALSPYILIFQ
                     TAPKISLAPLFVLWFGLGILSKVVLIALVTLFPVMINMILGVRSTEKNYYHLMRVLGA
                     RPWQVMLKLELMNSLPYLMTGLRVGLVLSITAAVIGEMMGSRAGLGFLLILGNEMYDI
                     TLLMTVILVISLLSFLLDVGMKKLQDRLLSWHESSAK"
     gene            8872..10137
                     /locus_tag="PLD15_04240"
     CDS             8872..10137
                     /locus_tag="PLD15_04240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HPB63144.1"
                     /translation="MARIVSEISAGRGSMLDSIREKIIREIEAFARFSLSGPGVTRLP
                     FSYENDGTIEYIRERLRSLGKGVYVDELGNVASSSGNVVPADKTYIISHYDSVPEGGK
                     YDGVAGLVFGLILLEMLEGRARDSVEMIAFNCEESSLFGRASIGSKYFFGGGRMPENY
                     PALIGGERSLREMMDIKHYSKLSLAEKPEIEESSRFLEVHIDQSACLYRKGSRLGLVE
                     RIAGQRRLRLTFTGETGHSSLADLSARKDSLLPAASAIRAVRELMEKHLISGAVGTVT
                     RVENRPNVMNMIPGETELMVDIRGFSVEALKVYVEELVDICRGESERYSIAFDWSAVS
                     QYDPAVMNGEFSGRYFKQLSESGLNPIVMNSMAWHDIAGTAGIYPSNLLLLPNPSGVS
                     HSPDESMDIDACASLLEFFMAKGVLCASD"
     gene            10124..>10960
                     /locus_tag="PLD15_04245"
     CDS             10124..>10960
                     /locus_tag="PLD15_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB63145.1"
                     /translation="MLLIKNVRMVDWRRSSVTPYDILIEGDRISKIESEINLSGVPVI
                     YGAGLTAIPGMVDAHVHLRQPGYEYKETVETATRAAAAGGVTALCAMPNVKPCPDSPE
                     SLENARSFQRDSKVRIIQSCAINRDLNGERSDWKELLASGARFFTNDGLPVDRVSDMV
                     EILKFSRETGVVVGEHPEAPGMDEKLHRAETMMICRDIFLNEKIGGNLHIQHVSLADS
                     VRYLEKCRHKNIKFTIETCPHYFREVEKGLDGGMYEVYPPLRNEEDRRAIIWALSTRL
                     ISI"
BASE COUNT         2597 a         2798 c         3084 g         2481 t
ORIGIN      
        1 cagcgccacc ctctgctgct gaccgcccga gagcttggag ggcggccgct gggcgagatc
       61 tctcaggccg acgagtccca gcgtattcat gactttttcc tttatctgtt tagaaggcac
      121 ctttctcaac ttcaggccgt aggcgacgtt gtcgaagacg ttcatgtgcg ggaagagccc
      181 gtagctctgg aaaacggtcg ccgtgtctct cttgtttgga gggagaaagg tgatatcttc
      241 gtttcccaga aagatcctgc ccttcgtggg aagttcgaat ccccctatca ttctaagcgt
      301 ggtggtcttg ccgcaccccg aaggccccag gagagtgacg agttctcccg gttcgatttc
      361 gaagttcgcg tcgttcactg cgacgacgtc ggcctttcct cttaggtctt tgaagatctt
      421 cgttacgctt tctattctca ccgaaacgga agcctttgac attcaaccac cccgcaacag
      481 gatcagttaa gattgacgct cttttcaagg tattcgttct ttcgcaccaa aagcctcata
      541 aggccgaaag tggcgaagac tataataatt agaaccgtcg aaagcacgct ggccagaccg
      601 aaccttatgg actcggagaa attgtatatc agaacggtca tgtggtacca ctgagccgag
      661 atcaggaata ttatggcgct cacggccgtc atggatctga cgaaggtgta ggacataccc
      721 gagatgaagg ccggccttat gagcggcagg actatcgtcc tgaagaccgt tgtcgaatcg
      781 gcgcccagat cctgcgccgc ctcctctatc gccggatcta tctggcgaag cgtggcgacg
      841 ccgccctcga ctcccaccgg cagctccctg attatatagt tgataacgat gatggccccc
      901 gtgccgatca gtatgagcgg cggttcgtcg aaggccagta tgtaggctat accgatgagc
      961 gttcccggta tggcgaaagg cgcgagaatc agtccttcga ggactctctt gccggcgaat
     1021 ttcttcctca cgatcaccag cgcggccatc atggcgatca atccggcgat gggagtggct
     1081 atcgccgaaa gcgtgaaagt gtcgaatatg gcgtcgcgcc ccctctggag cgcctcggtg
     1141 atgtttttca gcgtgaagga gtaatcgatt ccccagttgg ccacgaaaca gcctgcgacg
     1201 atcgttccgt aaaggccgag gatgaagact atgaaaagag agatgaaggc caaaagcgag
     1261 tattttaccg ggcgggaaac cagatcgctg aggcgtgccg aaggctttcc agtaacggtg
     1321 acatatgact ttctgctcac ccagtatctc tgtaccagaa aggccgtgac cgaaggcaga
     1381 agcaatagaa ccgacagagc ggcgccgtgg cccagcctgt tgcggccggt cacctcgata
     1441 taagcctcga cggagagaac cctgtaggag ccagccagaa gcaggggatt ggcaaaatct
     1501 gccagcgagt tggtgaagac tagaagccac gaactgagca ccccgggaat cgccagcggg
     1561 aaggttatcc gggagaaggt cttccatctg ctgccgttga ggtccagcga ggcttcctcg
     1621 agggtggaat cgatggcctg aagaacgccc gacaacgtca ggaaggcgat gggaaacatt
     1681 ccgatcgtct ggacggccgt aagaccgccc aggccgtata tggtgaaatc cttcaatccg
     1741 agtatctgcc tggttataag accattgctg ccgaagagga ggatcagcga cagcgacagc
     1801 gagaagggcg gagagatgac cggcagaagc cccatcgtac tgaggaagcg tttccccttt
     1861 atcgcggtcc ggtttatcac gaaggcgaaa atgaacccta tcaccgtgga gaaggtggcc
     1921 gtaagcatgc cgagtttcac gcttccccag agcgcgccga ggtactgcct ggaggtcagg
     1981 atcgaaaccc aggtgtccat ggagaagttc ccatccttca gaaaggtgag acgtacggct
     2041 tcgaagagcg gatacgccac gaagatgccc accattatga agagtcccag gattatatag
     2101 cccatcgccg ggtcgcggaa gaacttcgaa atgaaataaa cgatcacgaa gacggcgaaa
     2161 gcgccgatca tgaagatatc caggagccgg gcgtagttct ttccgccctc ggccgacatg
     2221 aggaagacca gaaaacccgt tatatcgtag cctgtctcgt cgatcaacgg cgagaagacc
     2281 agggaaacgc gttgatcgtt gaaagagtag tccaggcccg aaaagtagtt ttccagataa
     2341 tagacgctct cgtacccctt ttcgaaatcg gccgagccat tgtattcatc gaagaagctc
     2401 ttcaacttcg ggtccggcgc gaaaacggtc aattcacccc agccaggtac accgtcaacg
     2461 tatatgatat cggcatcggt gaactctctg ttcgcggcga ctatccagct ttcggccagt
     2521 tcagcctctc tgggcgccgc ctcggagaga accgtgacca gctgcctctg gttatccctg
     2581 gttattttgt agaaggattg cttcagggta ttgaaaatcc ataacagtac aacgaaaacg
     2641 gcgacactca agactacgaa agaaagaagc tttttccatg aatacaaagc gatcacatcc
     2701 caaagtcgca catggtttca tgtataaccg gaagggggcg gtcgcgaccg ccccctgtta
     2761 actcaaaggc cggcccctat ctccctggtc cagcgatcga gaagcctctc tttgttctcg
     2821 gcgtcccaga cgtaatcctg gtgaacggtc tttatctcgt ccatgctgaa tgatagcggg
     2881 tgctttgggg ctatcttcga gacgggaatg acataccagc cggcgattat gccctgcgcc
     2941 tcttcggtca atatccagtc gtagagcttc ttggcgttca ccggatcggg accgttcttt
     3001 atcagcgaca tggaagcgat ctcgaaacct gtgccctcct cgggaacggt gattataaca
     3061 ttggcgcctt cggccttgag cttcaccatg tcatgggcgt agccgatcgc cacagggatc
     3121 tcgccgatgg caacgctctt gccgccggcc gaaccggaac gcgtgtacat atcgatactc
     3181 ctgtcgagct tcttcaggta aaggaaggcg agatcttcat cgccgccgaa gagcgatatc
     3241 atgcaagtga tgaggttgta ggccgttccg gatgtgctgg gatttgccac tctcacgagg
     3301 ttggtgtatt tggagtccag aatgtcgaac catcccttcg gaggctctat gcctagctct
     3361 ctggctctat cgacgttcgt gacgaaggcg agaggcccaa gatacagtcc tatccagtaa
     3421 ccctcggggt ccttgtactg ttcgggagtg ttggcggccg ccctggaaaa gtacggcgtc
     3481 gtgagcccct tgagcttggc gcttatgtgg ttgagtccga ctccaccgac ccatatcgag
     3541 gcctgtgggt tcatcttctc ggcctccatc ctggcctcgg cctctcctcc ggaaagcctc
     3601 acccaatcga cccttattcc ggtcacctct tcgtaccggt cgaaaagctg tttcgcggcc
     3661 agctcctcga gagtggtgta aactgtaaaa gattcggctg ccagagccgt tgagaagacc
     3721 atacagacga taccgataac aacgataaat ctcttcatcc cgggaaaacc tccttgaatt
     3781 cttcagattc acgtgatcga cagctctatc tgttacggtg cgtattacat aacgcatgcg
     3841 gaactcttaa taattatacc attaattaaa gttgcgaaaa acttagaaaa ttcgttccag
     3901 agggttttct ctgtccataa tcgaccgtgg tacccttcaa aggcgaattg atacagagaa
     3961 aaaataggta taaaattatt ctatatggca gaatagaaat tccgggagga ggaatatgaa
     4021 gtatctccag aaagtcttga atgtcatctg catagtcgct tattccgata ttgagggtgt
     4081 ttcggtcatt gaagtatcgg agaaagcggg tttgcctatg tcgtcaacgc acaggattct
     4141 caacgatctc gtggaatgtg aagctctggt gaagaacatg gagagcagga agtacaggct
     4201 cagccccagg attgtgccgc tggttcacga gatttccagg aagatgaatc ttgattggct
     4261 gaaagtgatt ttgacggagc tgaaggacga gataaacgag accgtctttc tcagcgaatt
     4321 gatggaaaat ggagtttcgt ctttgcttac ggtcgaaagc aacagagtct tcagcttcag
     4381 ggcaagatcg ggagtttacc tccccgtcca ctgcaccgcg gccgggctgg cgatagttgc
     4441 ctacatcgaa ccgagcagag ccgaaaagct catgtgtatt tccagcagtt atagcgattc
     4501 acaggtaagc cagtgcccgg tcgaggagtt cagagatagg cttgcgaagg tccgaaggga
     4561 tggattcgct ttctgtaacg aggagtatga accgggattg agagcactgg ccgttcccgt
     4621 attgaggccc aacggctttg ccttcgccag cataaccgcg atcgctccgg gcgagaggtt
     4681 cagcgacgag gaaggcaaca ggcaggttgt tgaacgtatg aaggaagcag ctgtcaaggt
     4741 cggcaaattc atgtattaga ggtttgtatg agtgaagaag tcgtaagggt gaagaacgtc
     4801 ggtagaacct ttgttagcgg cggcaagaag gacaggaaag agtttacggc cctggagaag
     4861 gtctccttcg aggtcggcag gggcgagttc gtctctcttc ttgggccttc cgggtgtgga
     4921 aagtcaacgc tgttgaagat aatttcggga ttgctgccgg ccacttcggg aagcgttgag
     4981 ataaccgaat cggtcaacga aaaggtcccg cccttcggtt tcgtctttca ggactccgtt
     5041 cttctcccct ggcgcaccgc tctcgacaat gcgctctttc ccttcgagat aatgggaaac
     5101 agggagaaat ccgacgagaa acacgttaga gaactcttcg cgctggccag gctggtcggg
     5161 ttcgaagagt cctaccccag acagctttcc gggggaatga aacagcgagt gtcgatagtc
     5221 agagcgctct cctacaatcc gccgattctt ctgatggacg aacccttcgg ggcgctcgac
     5281 gccataacca gggacaacct gaacaatgtc ctgctggaga tatgggaatc gacaagaaag
     5341 accgtcatct tcgtcaccca cagtatcagc gaggccgttt acctttcgga caggataatc
     5401 gtcatgggta cgaagccggg cagagtgctg aaggatatga aagtcaccct gcccaggccg
     5461 cgcaacgagg ataccaaact ctctccggag ttctacgctt atgtaaggga actcagggag
     5521 atgctcaaat aaagacggac gtggtgctcg atgaataaga gggttttaat aaccgttctc
     5581 tcgattctct ttctcttcct ctttcttggc atctggaagg ggtatatagt tctcaacgac
     5641 gtgccgccct tcatcctgcc gccacctgag gcggtcggcc agaggttcgt cgacctgatc
     5701 gccgacggca ccttcatccg cgagggttgg gtcacgctgt acaccataat actgggcttc
     5761 tcgatcggtt cggcgctggg ttttttggcc ggttacttct cggcccggtc caggaccttc
     5821 gaggagataa tctctcctta cgtcatggtc atacaggcca cccccaaagt ctctttgatt
     5881 ccgctcttcg tcatatggtt cggtctgggg atgccatcga agctacttct tatagtcctt
     5941 tcggccttct tcccggtgat ggtgaacact attctcggcc tccgttcgat cccagaggat
     6001 tacagcaact tgatgaccgt tctcaaggcc tcagaggggc agcgggtttt gaaactcgag
     6061 cttccgatgg ccacgccgct tataatggcc ggactcaaga tcgccatggt tcagtcggtg
     6121 atcggcgcta tcgtctcgga atggatggcg ggcgaccggg gcctcggcta ccttctggtt
     6181 tatggaagtt ccctgtacga tgcgaatttg ctcatggctt cgatactggc caccaccttt
     6241 ctcggtctgg ttctttactg gagcatagaa ctcgtagaga agaagttgct cttctggcac
     6301 gaatctaaac tggccttcac ggagggaacg tgatggatga taagaagttg aaatcgatag
     6361 ccggcaaccc cgttgatgac gggcttctgc cgcaccttaa ggttcgtacc gacctgaaag
     6421 tgcccgagtt gttcttcctt cccggcgacc cggctagatt gaagctgttc cgggaactgt
     6481 cggacgaatt ccagttgctc aacgagaaca gggaattctc gatagctgtc gggagttatt
     6541 caggaagacg ttttggagtc tgttcaaccg gtatcggtgg cggctcgtcg gagatagtca
     6601 tggtcgagct gaaagagctt ggagtggaaa aagtcgtaag ggtcggcggg tgcggcgctc
     6661 tgagggaaga tatagagtgt ggcggcatgg tcttgaattc cggcgtcgtc aggctcggag
     6721 ggagttcgaa gatgtacgtg agacccgagt acccggctgt ggccgatccc ttcatgctgg
     6781 tcgcccttca cgaaagcgtt ttgagggccg gaaagaaggt ctgcgtcggc attggcgcta
     6841 cggtagattc ctactacgcc ggtcagggca ggttccttcc ggccatcgaa ggtctgggag
     6901 agagggatct tctggagaga atgagggcgg ccggagtggt cgacttcgac atggagagcg
     6961 aaacgatatt cactctggcc agcctgatgg gcatgagggc ggccaacatc cttgccgtcc
     7021 acggcaacag ggccagcaac atgtggttga gcgactacag gagcgcgcaa atcgatgcaa
     7081 taaagatagc tctggaggca gagctttgaa actacaaaag aggagggatt tgttttgaag
     7141 aaggcagctg tcattctact ggttttgttc ataagccttt tcgcgttcgc acaaaaggtc
     7201 acgatccaga tcgaaggctc ggccgttccg tattacattc cgatctacat gggcatcgag
     7261 ctcggctact tcaaagaaga gggcctcgag gtcgagtatt tcttcggtaa cgcttccgat
     7321 atcataagga acgttgcggt cggaaacgta gaattcggtt ttccgaacgg agagcccgta
     7381 atagtcgcaa gatctcaggg tattcctgtc aacgtcattc acacgactta ccagcacggc
     7441 ctgggctcca cgattttcct caaggatagc ggcatacaga cgccgacaga cctgaaggga
     7501 aagaaagtgg ccataaccag ttacggtagc tccaattacg tgcagataca ggtattgctc
     7561 aagaagaacg gtatgactct caacgatatt cagctggaga taatcggtac cgaggctatc
     7621 gttccggccc tcgtcaacaa gagagtcgac gcgatctgtt tctccatgct gaggactttc
     7681 gagctcaggt accagggcat agaggtcgaa gagttcaggt ccgacgagtt ccttccgtcc
     7741 ttcggaaatg tgctggtgac cggcgacaag attctcaagg ataataagga cctggccgtg
     7801 aagttcgtca gcgcgctcaa caaagtcatg acttaccttt ccgacactga aaacatgctc
     7861 aaggccacct acacggcaat cgagaagtac acgccgacct acgcaggccg cgaagaatac
     7921 atggccgcca tacttagcga tatctacgcg gggtatctct ggcagagcga agacacggag
     7981 gctttcggct tcggttacgg aaacgtacag aggtggcagg actcgataga tatcatgaag
     8041 gagtaccaga ttataacctc cgacgtcaag gcctccgatt tcatcgtaac gggtctttga
     8101 ttggtctctt tcggagtggg ggaggattat gaagaagaag ctgggaatca aattgatgag
     8161 ttttctcgta cttgtcgcca tagtggtggt ctggaaagtc atggtgaccc ttctgaaaac
     8221 gcctacgcac atcctccccc ctccggaaga tatactcttc aaattcatag aactcataag
     8281 gaactccgtt ctccagaaga acttcctggc cactttcgaa gagatcgtga taggtttctc
     8341 cgcgggcgcc ggacttggaa tagttatagg ttacatcctg gccaaagtcg aactgctgga
     8401 aagggcgctc tccccttata tactcatctt tcaaactgcg cccaagatat ctctggcacc
     8461 tcttttcgtt ctgtggttcg gtctgggaat actctccaaa gtggttctca tcgcccttgt
     8521 aacgctcttt cccgttatga tcaacatgat actgggtgtc aggtcgaccg aaaagaacta
     8581 ctaccatctg atgagggtgc tcggggccag accgtggcag gtcatgctta agctggaact
     8641 gatgaactcc cttccctatc tgatgaccgg actcagggtc ggcctcgtcc tctcgataac
     8701 ggccgccgta ataggtgaaa tgatgggttc aagggccggc ctgggctttc ttctgatact
     8761 tggaaacgag atgtacgata tcaccctcct gatgacggtg atactcgtga tcagcctttt
     8821 gagtttcttg ctggatgttg gaatgaagaa actccaggac aggcttctct catggcacga
     8881 atcgtcagcg aaataagcgc gggcagaggt tcgatgctcg actcgatcag ggaaaagatc
     8941 attcgcgaaa tcgaagcttt tgcgcgcttc tctctatcgg ggccgggagt tacccgcctg
     9001 cccttctcat atgaaaacga tggaacgatc gaatatatac gcgaaaggtt acgctctctg
     9061 ggcaagggag tctatgtcga tgaactggga aatgttgcca gttcctccgg aaacgtcgtt
     9121 ccggccgaca agacttacat aatctctcat tacgactccg tgcctgaggg aggaaagtac
     9181 gacggtgtcg ccgggctggt cttcggactg attcttctgg agatgctcga aggtcgggcg
     9241 cgcgattcgg ttgagatgat cgccttcaac tgcgaggagt catcgctctt cggacgggcc
     9301 tccatcggat cgaagtactt cttcgggggc ggacgaatgc cggagaacta ccccgccctc
     9361 ataggcggcg aaagatcgct ccgagagatg atggacatta aacactactc gaaactctct
     9421 ctggccgaaa agcctgaaat agaagaaagc tcgaggtttc tggaggttca catcgaccag
     9481 tctgcctgcc tttaccggaa aggctccagg ctggggctgg tcgaaagaat agccggacag
     9541 agaaggttga ggctgacctt cacgggcgag accggacact cgagtcttgc cgatctgtcg
     9601 gcgaggaagg actctctgct tccggcggcc tcggcgatca gggccgtcag ggagctcatg
     9661 gagaaacacc tgatttccgg cgctgtcggt acggtgacga gagtggagaa caggccgaac
     9721 gtcatgaaca tgattcccgg tgagacggag ctgatggtag atattcgcgg tttctccgtc
     9781 gaagcgctga aggtttatgt agaagagctg gtcgatatct gccggggcga gtcggagagg
     9841 tactcgatcg ccttcgattg gtcggcggtt tcgcagtacg atccggcggt catgaacgga
     9901 gagttttcag ggcgttactt caagcagctt tcggagtccg gactgaaccc gatcgttatg
     9961 aacagtatgg cctggcacga tatagccgga acggccggga tttatccttc caatctcttg
    10021 cttctgccca acccttcagg agtgagccac tcaccggacg aaagcatgga tatagacgcc
    10081 tgcgcgtcgc tgttggagtt ctttatggcg aagggggttt tgtgtgcttc tgattaagaa
    10141 cgtgaggatg gttgactgga ggagatccag tgtcacacct tacgatattc tcatagaagg
    10201 cgacaggata agcaaaatag agagcgagat caacctttct ggcgtgccgg tgatctacgg
    10261 cgccgggttg accgctatac ccgggatggt cgatgctcac gttcatctaa ggcagcccgg
    10321 ttacgaatac aaagagaccg tcgagacggc gaccagggcc gcggcggccg gcggagtaac
    10381 agcactctgc gccatgccga acgttaagcc ctgtcccgac agtcccgaaa gtctcgaaaa
    10441 cgcccgttcc tttcagaggg attcgaaggt caggataatc caatcgtgcg ccataaaccg
    10501 cgatctcaac ggtgagagga gcgactggaa ggaactgctc gcaagcggcg cgaggttttt
    10561 caccaacgac ggcctgccgg tcgacagagt ttcggacatg gtcgagatac tgaaattctc
    10621 cagagagacc ggcgtggtgg tcggcgagca ccccgaagcg ccgggaatgg acgagaagct
    10681 ccacagggcc gaaacgatga tgatttgcag ggatatcttc ctcaacgaaa agatcggcgg
    10741 aaacctccat atccagcacg tcagtctggc tgattcggtg agatacctgg aaaaatgcag
    10801 gcacaagaac ataaagttca ccatcgagac ctgcccccat tacttccgcg aagttgagaa
    10861 aggcctggac gggggtatgt acgaagtcta tcctcctcta aggaatgaag aggaccggcg
    10921 ggccataatc tgggcgctct cgacgcgcct cataagtata
//
LOCUS       DAOOLY010000054        10921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6789_length_10921_cov_4.960795, whole genome shotgun sequence.
ACCESSION   DAOOLY010000054 DAOOLY010000000
VERSION     DAOOLY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6789_length_10921_cov_4.960795"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLD15_04250"
     CDS             complement(<1..238)
                     /locus_tag="PLD15_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63146.1"
                     /translation="MIRILEKRKSLLEDIISRAKYSVSRDLNTVIENDVVLFIDTVPD
                     EDILSKTIGRVPVVLYGSAVKALQLIGVEEIGAIE"
     gene            complement(252..1283)
                     /locus_tag="PLD15_04255"
     CDS             complement(252..1283)
                     /locus_tag="PLD15_04255"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPB63147.1"
                     /translation="MFDLEDLSQLRNESSRSISAENPDGRVSGGALEDPRGSGPARRL
                     GKGWKARPCIDIQPGETVELAKITGPGTIRHVWMTVDSKAYRTCLIRFYWDGEPFPSV
                     EAPLGDFFACTHGLRYSVNSLPVAVNPSGGFNCYWPMPFREGARITLENLAAKPVQNF
                     FSQLDYSLGQVPESSAYFHASWRRSVTTRENPEHIILDGVSGSGHYVGTVAAWTQFSN
                     GWWGEGEVKFFLDGEDYPTICTTGTEDYFGGAWCFGETFSAPFCGYPLWRREEGEVPR
                     HGLYRWHICDPVRFRKSIKVTVQALGWYPDGTFQPLTDDIATVAYWYQNEPHLNFAAE
                     YTPQELWSR"
     gene            complement(1309..2148)
                     /locus_tag="PLD15_04260"
     CDS             complement(1309..2148)
                     /locus_tag="PLD15_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63148.1"
                     /translation="MRDNKIMTIITRIVIMALLILLGLSTLLPYILMISFSFMAEFEM
                     YRLPPKLIPDVLRLENYVEMWKSQPWGRYIFNTLFVTICVLIGQIILVAMGGYALSRL
                     HFPGRDFMFKLFITFMLLPGVVTLIPGFIILHSLDWVDTYWAMIVPALGNIWGMFLMR
                     QYMLSLPSSIEDAARIDGASTWQIFWRIIMPLCKPVIATVGTFTFLGTWRSFFWPLIV
                     TRSKEMRVIEVGVAMFSSQYTTNYPAQMAAAALSSIPMILIYVIAQKWIVQGIRLTSG
                     FDK"
     gene            complement(2165..4438)
                     /locus_tag="PLD15_04265"
     CDS             complement(2165..4438)
                     /locus_tag="PLD15_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63149.1"
                     /translation="MRHNKLLIGLFIFVALLMLSGFAILNLYAADGYSSYARESESSL
                     LKSMKLNYQNLAAGIDGELYPLVDDPQAVGSVVEGLSDDIVFAALYDLDGKLLEDFGY
                     SVPDDLSSSIFFENLTFSSGLPRAEVSRFNDKLYIQAGILTLEDTVFVVLLDRFDGLL
                     TNSLSAYDRELLVYYGDEDSFKAPAGAPKIDQALLRDLFKRTMNSQRPESAEISSADG
                     EMIVNALAIYDSDNWDVISFFSTVTTPAIWKRGIGSLAVFIWVSGIASLIFALFLLYV
                     YFKKVQKDPKMKKSTRFLYCLSAFLPAIVMVAVFGYVVYGESLEALSDAVAMKNARGW
                     FGDVERFLEVEGADSPAEFIERSRETTGANFVMREGGSLLASNLTERRLSNLEIVSST
                     TIEGGIVKGTAKLNDVLHTYATGEISGIEATVLYEETSRENAVLSIQFFAIGMILTLL
                     VMAVVSGFIINNIQSSILIKRTFIGYGFLMPALVHLIWWAVGPMGFALFLAFRRWSIV
                     DAAKPFVGLENFIEIFGDFKFWNALKNTAVYSIYVPIGMFISLLLAIAVNKLGKLSIV
                     LRVLYYLPVVTAGVATTIVWRWIFNRDFGVLNFILGLVGIKPIAWLESPQFALISMMI
                     IGVWSAIGSQLLIFLAGLQGIPKDFYDAASVDGASKPKIFWKITLPLLKPTSLFVLVT
                     SVIGSFQVFTPIYVLTQGGPLRSTDVVFYHIWQSAWVDMRMGYAAAQSWILFLLLAVL
                     TFVEFKLFGKESWQAYF"
     gene            complement(4498..5799)
                     /locus_tag="PLD15_04270"
     CDS             complement(4498..5799)
                     /locus_tag="PLD15_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB63150.1"
                     /translation="MDRARYSRFFLVVVLLVLSSLAIGATKLRVTTWAGADEAALDEA
                     IIAEFMRLNPEYEVVYEPVPGDYYQKILTDIGAGTPPDVILLDAEMIPAFVEGKYLTD
                     INPFISRLSREGASGTNLDEYFPVLVDIFRSGRSLYALPKDTSPIGIFYNKNVFDQFG
                     VPYPPENGWTMEEFAETVKKLSKDTDGDGKNDVWGFSFPSWVGVVVPILWAGGGEVFS
                     PDFTKTSGYLNSDVNVGTYNFYMDLLAKGYAPTPQEASSLGGASALFYTGKVAMIITG
                     RWFNVSIKSQIAKGADLKVGGASIPYASEESKATITYASGWAVPSNVADKSGATKLAA
                     FLSSEYAQTKRCLEGGLAVSAIRSIAELQAAKDALDSTFIGMLDYARVPVGSLTKYYR
                     PQFEDTWAEAFDRMNVGGETVKQAMDWAAATIDEYIEKGEK"
     gene            complement(5850..8039)
                     /locus_tag="PLD15_04275"
     CDS             complement(5850..8039)
                     /locus_tag="PLD15_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63151.1"
                     /translation="MEIDILKMLERKDKWYLGGGNRLLWTPPFPLHLDRPGAWDFVSY
                     FDLRIDPGYTISVIDRGVPVDFICIDRLWNPASLSSLFRAGDLEMLEEKALLKEDFLA
                     SRVTLKNDSKEMKELDIVVWTAQQCSDSDSEVDTQIIDAVGSGIVFKKRLVKPRRRPY
                     PAHVILALEGKESHNVSFSERTGNLPNFRLTPFYEKLERDGLIERIDTNGINREGLLY
                     IGLHRKVKLAPGSETTFNCGLGAAPGVEEVVRAAGRSLGCDPVEESRRNWESYFRFLP
                     RFSIGEPYFQKYYYYRWYGLKLFTGEVDEAYMKHPAIAEGLDYFRAFITYSAQCHMLE
                     TRWSASPEVARGSLLNFIENQREDGSFAGHIYVNQLQENGFYHADWGRAVMELHRVHP
                     DRSFIERIYEPLKKYLAYFDRVRDREGCGLYDVVDQFETGQEYMSRYTAVDPMADRYG
                     WINNIRFKGVDATVYVYNLKKALAWMAGLLEMKEERMFFEESAAKTKKAVLELMWDGD
                     EEIFSDVNPVDFKRTGVKAAVCFYPYMTDIVDESHLPGLKKHLLNREEFWTEYPVAST
                     SVDDRLFSQWAEWKGKRHNCPWNGRVWPMTNSHMVDVLGFCARRFGDRLLREKTVELV
                     EKFIKMMYYEGDIERPNCFEHYNPFNGKACVYRGVDDYQHSWVVDLLFRYLAGIDFTE
                     DGPMLDPFPFDIDFELDNLVIAGKELRIRSEKGIFEAQYDGKRINRL"
     gene            complement(8029..8769)
                     /locus_tag="PLD15_04280"
     CDS             complement(8029..8769)
                     /locus_tag="PLD15_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPB63152.1"
                     /translation="MAMKTKKRTSTDVVNRLKKMIVLEDLERLPGEVELSSRLGVSRT
                     IIREALRVLEYEGVTRTVHGSGTFVLKRTSLKILFNVNFEIETDSARDILDLIELRST
                     LERSAIALAVSHASPADLEELSHCMDRLSRAISTRENLGDTDACFHKKLFDISHNRFL
                     KEVFDVVFDGLEVLWKSPLGLDTFGDAGLPLHRDLYEAIRERNLDAALEIYDRIISLD
                     RKDIIDLTGYEPKSQMQHSITGVKRDGD"
     gene            complement(9096..9437)
                     /locus_tag="PLD15_04285"
     CDS             complement(9096..9437)
                     /locus_tag="PLD15_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB63153.1"
                     /translation="MVDTKNTNPVVFFSVITGDCSQSKGDEMNGFDFLAGLRSDDRYR
                     ELRVIILTSPDEIDNEIEGVELGATDYIRKPISGEMELRQNTGYLISEPLNEKAAIEF
                     LREYNYQQQLP"
     gene            complement(9626..10549)
                     /locus_tag="PLD15_04290"
     CDS             complement(9626..10549)
                     /locus_tag="PLD15_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63154.1"
                     /translation="MDTLKVVNLKARIGTFELGPVDLNVGRKEIVGLIGPSGCGKTLL
                     LRTIAGLQEPVAGQVFIDGKDVTYLEPHLRGLAFVFQDNALFPHFDTHNNIAFPLKIR
                     KDPQTEEKVQKKAAELDGLADYLDRWPKELPAGMKKLTAIGRETVKKFNLILMDEPFE
                     RLDKKIRTELRAMIKRMLMAIGESVLIVLNDPEDALAITDRVYVMNDGVFVQSGGPRE
                     VYASPASHFVMELFSTMGINRVGGAVFRPQDVEIDENGSYAALEHCGPYDSKHVLCNA
                     KMEGKDVVLLLPVECREKPGVKIRIKNSFKN"
BASE COUNT         2439 a         3022 c         2798 g         2662 t
ORIGIN      
        1 cctctatcgc ccctatctct tcgacaccga tcaactggag agccttcacg gcagagccgt
       61 aaagcactac cggcaccctg ccgatagttt tagatagtat atcttcatcg gggactgtat
      121 cgataaatag aacgacatca ttttcaatca ctgtatttaa atcacgactg acactgtatt
      181 ttgcccttga aatgatgtct tccaggaggc ttttccgctt ttcaagtatt ctaatcatat
      241 cggcccgtta cctacctgga ccagagctct tgtggggtgt attcggcggc gaagtttagg
      301 tggggttcgt tctggtacca gtacgcgacg gttgcgatat cgtcggtcaa aggctgaaaa
      361 gttccgtccg ggtaccagcc gagagcctgc acggtaacct ttatcgattt tctgaatcta
      421 acggggtcgc atatgtgcca gcggtagagg ccgtgtctgg ggacctcgcc ctcctctctt
      481 ctccagagcg ggtacccgca aaaaggcgcg gagaaagtct ccccgaaaca ccaggccccg
      541 ccgaagtaat cctccgttcc agtcgtgcag atggtcgggt agtcctcccc atcaagaaag
      601 aacttgacct ccccctctcc ccaccagccg ttcgagaact gcgtccaggc agctaccgtg
      661 cccacgtaat ggccggaacc gctgactccg tcgagaatta tgtgttcggg attctcgcgg
      721 gtcgtcacag acctccgcca ggaggcgtga aaatacgcgg agctctctgg aacctgaccg
      781 agcgaataat ccagctggga gaagaaattc tggacgggtt tggctgccag attctccagc
      841 gtgatcctgg cgccctccct gaaaggcatc ggccagtaac agttgaagcc gcccgaaggg
      901 ttcacggcga cgggcaggga gttaacgctg tacctcaggc cgtgcgtgca ggcgaagaag
      961 tctcccaggg gagcctcgac cgaaggaaag ggttcgccgt cccagtagaa ccttataagg
     1021 caggtcctgt aggctttgct gtcgaccgtc atccagacgt gcctgatagt tcccggtccc
     1081 gtgattttcg ccagttcaac cgtttcaccc ggctgtatgt ctatgcaggg tctcgccttc
     1141 cagccctttc ccagccttct cgcaggcccg gagccgcggg ggtcttccag agcgcctccc
     1201 gacacccttc catcgggatt ttcggccgat atggatctgg acgactcgtt tctcaactgg
     1261 ctaagatctt ctagatcgaa catttatctt cctccttgaa tttatatttc acttgtcgaa
     1321 tcccgacgtg agccttattc cctgcactat ccatttctga gcgatgacat agatcaggat
     1381 catggggata gaagagagcg cggccgccgc catctgcgcc ggatagttgg tagtgtactg
     1441 cgaggagaac atggccactc ccacctctat taccctcatt tccttgcttc tggtgactat
     1501 caggggccag aagaacgagc gccaggttcc cagaaaagtg aaggttccca ccgtcgcgat
     1561 gaccggtttg cagagaggca tgattatcct ccagaaaatc tgccaggtag aggcgccgtc
     1621 tatcctggcc gcgtcttcta tactgctggg aaggctgagc atatactgcc gcattaaaaa
     1681 cattccccag atgtttccca gcgccggaac gatcatcgcc cagtaagtat ccacccagtc
     1741 gagagagtgg agaattatga agcccggtat cagcgtcacg acgccgggca ggagcatgaa
     1801 agtgatgaag agcttgaaca tgaagtcccg gccggggaag tgaagcctcg aaagcgcgta
     1861 tccccccatg gccaccagta ttatctgtcc gatcagcacg cagatagtca cgaaaagcgt
     1921 gttgaagatg tatctacccc acggctgcga cttccacatc tccacgtaat tctccaggcg
     1981 gaggacatcg gggatcaact tgggcggaag cctgtacatt tcgaactctg ccatgaaaga
     2041 gaaggagatc atcagtatgt acggcaggag agtggataga cccagaagaa tcaaaagggc
     2101 cataataact atcctggtta taatcgtcat gattttgttg tctctcatac cctcactccc
     2161 cttctcagaa gtacgcctgc cagctttctt tgccgaagag cttgaattcg acgaaggtca
     2221 gaacggccaa caaaaggaag agtatccacg actgggcggc ggcgtacccc attctcatat
     2281 ccacccaggc cgactgccag atgtggtaga aaacgacgtc ggtcgatctc agcgggcctc
     2341 cctgggtgag aacgtatatc ggcgtaaaga cctgaaagga ccctatcaca ctcgtcacca
     2401 gtacgaagag gctagtgggc ttcaacaacg ggagggttat cttccagaag atcttcggtt
     2461 tgctcgctcc atcgacgctg gcggcgtcgt agaagtcctt ggggataccc tgcaggccgg
     2521 ctagaaagat cagtaactgg cttcctatgg ccgaccacac accgattatc atcatcgaga
     2581 tcagcgcgaa ctggggcgat tcaagccagg ctatcggttt gatccccacc agcccgagaa
     2641 tgaagttgag aacgccgaag tcgcggttga agatccagcg ccagactatg gtcgttgcga
     2701 cgccggccgt gacgaccggg aggtagtaaa ggacccgcaa aacgatagaa agtttcccca
     2761 gcttgttaac tgcgatagcc aaaagaagag agatgaacat gcctatcggg acgtagatcg
     2821 aataaacggc cgtgtttttt agagcgttcc agaatttgaa atcgccgaag atctctatga
     2881 aattttccag ccccacgaaa ggcttggcgg cgtccactat gctccatctc ctgaaggcca
     2941 ggaagagggc gaagcccatc gggccgaccg cccaccagat cagatggacc agcgccggca
     3001 tcagaaagcc gtaacctatg aaggtccgct ttatcagtat gcttgattga atgttgttta
     3061 ttatgaagcc gctcacgacg gccatcacca gcaaggtcag aatcataccg atcgcgaaga
     3121 actgtatcga aagcacggcg ttctctctgg atgtctcctc gtacagaacg gtggcttcga
     3181 ttccggagat ctctccggtc gcgtaggtgt gcagaacgtc gttgagtttc gccgtcccct
     3241 tcacgattcc tccctcgatc gtggtcgagg agacaatctc gagatttgaa agtctccttt
     3301 cggtgaggtt ggaggccagg agcgaacccc cttcacgcat cacaaaattc gcaccggtag
     3361 tctccctgga tctttctatg aattcggccg gagagtcggc gccttcaacc tccagaaagc
     3421 gttctacgtc cccgaaccat cccctggcgt tcttcatggc caccgcatcg ctgagagcct
     3481 cgagcgactc cccgtagacg acgtaaccga ataccgcgac catgacgatg gcggggagaa
     3541 aggccgacag acagtacaga aagcgcgtgg acttcttcat cttcggatcc ttttgaactt
     3601 tcttgaagta aacataaagc aagaaaagcg cgaaaatcag cgaggcgatc ccggaaaccc
     3661 aaatgaaaac ggccagagaa ccgattcctc tcttccagat ggccggcgtg gtcaccgtcg
     3721 agaaaaagga aatgacatcc cagttatcgg aatcgtagat cgcaagggcg ttcactatca
     3781 tttctccgtc ggctgaagat atctccgccg attccggccg ctggctgttc atggttctct
     3841 tgaagaggtc gcggagcagg gcttgatcga ttttcggcgc accggcaggc gccttgaaac
     3901 tgtcttcatc tccgtaatag accagaagtt ctctatcgta tgcgctcaga gagtttgtga
     3961 gcaagccgtc gaatctgtcg agaaggacaa caaacacagt gtcctcgagt gtcagaattc
     4021 cggcctggat atacagctta tcgttgaatc tggaaacttc ggccctgggt agtccgctgg
     4081 agaaggtcag attctcgaag aaaatcgaag acgacagatc gtcgggaacg gagtagccga
     4141 agtcctcaag cagtttgccg tctaaatcgt acagggcggc gaagactata tcgtctgaaa
     4201 gaccttcaac aacgcttccg acagcctgcg gatcgtcgac cagcggatac agttcgccgt
     4261 ctatcccggc cgcaaggttc tggtagttga gcttcatgct cttgagcaga gaggactcgc
     4321 tttcccgggc gtaactggaa taaccatcgg ccgcgtataa attgagaata gcgaaccccg
     4381 acagcatcag aagcgctacg aatataaaaa gacctatcag aagtttgttg tgcctcaaag
     4441 ccatccctcc cgaataagga aaggagcggc gcgatggccg ctcctcagga aaacctgtta
     4501 cttttcaccc ttctcaatgt actcgtcgat cgtcgcagct gcccaatcca tggcctgttt
     4561 gacggtctct cctccgacgt tcatcctgtc gaaggcctcg gcccacgtat cctcgaactg
     4621 gggtctgtaa tacttcgtca gcgagcccac cggtaccctg gcgtagtcga gcatacctat
     4681 gaaggtagaa tcgagcgcgt ccttggcggc ctgaagctcg gctatgctcc tgatagccga
     4741 gaccgcaagt cctccttcga ggcatctctt ggtctgcgcg tattcgctcg acaggaaggc
     4801 ggccagcttg gtcgcgccgg acttgtcggc tacgtttgaa ggtacggccc agccggaagc
     4861 gtaggtgatc gttgccttgc tttcctcgct cgcgtaaggt atcgaggcgc ctccgacctt
     4921 gagatccgct cccttcgcga tctgtgattt gatagagacg ttgaaccagc ggccggtgat
     4981 aatcatggca accttgccgg tgtagaacag tgccgaagct ccgcccagag agctggcttc
     5041 ctgcggcgtc ggcgcatagc ccttggcgag gagatccatg tagaagttat aggtaccgac
     5101 gttcacgtcg ctgttgaggt acccagaagt cttggtgaag tccgggctga aaacctcgcc
     5161 accgcctgcc cagaggatag gcactaccac gcctacccag gaagggaagg agaaacccca
     5221 gacgtcgttc tttccgtcgc cgtcggtgtc cttgctcagc ttcttgactg tctcggcgaa
     5281 ctcctccatc gtccagccgt tctccggcgg ataagggact ccgaactggt cgaaaacgtt
     5341 cttgttgtag aagattccta tcgggctggt atctttggga agcgcgtata gactccttcc
     5401 cgacctgaag atgtcgacga gaacggggaa gtactcgtcg aggttcgttc cgctagcgcc
     5461 ctccctgctc agcctgctta tgaaggggtt gatatcggtc agatacttgc cctccacgaa
     5521 ggccgggatc atttcggcgt ccagcagaat gacatcgggg ggtgttccgg caccgatatc
     5581 ggtcagaatc ttctggtagt aatcgccggg aaccggttcg taaaccactt cgtattccgg
     5641 attgagtctc atgaattctg cgatgatcgc ctcgtcaagc gcggcttcgt cggcgccggc
     5701 ccaggtggtt actctgagct tcgtcgcgcc tatcgcgagt gaagagagaa ccaaaagaac
     5761 aactactaga aagaaccttg aatatcttgc cctatccata cttccacctc cattttctat
     5821 tttccctgtc cgctctataa gcggtctttt cagagtctgt ttattctctt gccgtcgtac
     5881 tgcgcctcga aaatgccttt ttccgatctg atcctgagct ctttgccggc gatcaccagg
     5941 ttgtctagtt cgaaatcgat gtcgaaggga aaaggatcga gcatcgggcc atcttcggtg
     6001 aaatcgatcc cggccaggta tctgaagaga agatccacta cccacgagtg ctggtaatcg
     6061 tcgacacccc tgtaaacgca ggccttcccg ttgaagggat tgtagtgttc gaaacaattg
     6121 ggcctttcga tgtcgccctc gtaatacatc atttttatga acttttccac cagctccacg
     6181 gtcttctccc tgagcagtct atcgccgaac cttctggcgc agaatcccag aacatctacc
     6241 atgtggctgt tggtcatcgg ccagacccta ccgttccacg ggcagttgtg gcgtttgccc
     6301 ttccactctg cccactggct gaagaggcga tcgtcgacgc tcgtggatgc aacggggtat
     6361 tccgtccaga actcttcccg gttgagcagg tgttttttga gtcccggcag gtgcgattca
     6421 tctacgatat cggtcatata cggatagaaa cagacggcgg ccttcacgcc ggttctcttg
     6481 aaatcgacgg gattcacatc ggaaaagatc tcttcatcgc cgtcccacat gagctcgagc
     6541 actgcctttt tagtcttcgc ggcactctct tcgaagaaca ttcgctcttc tttcatttcc
     6601 agaagaccgg ccatccaggc gagggccttt ttcaggttgt acacgtatac cgtggcatcg
     6661 acccccttga aacgtatgtt gtttatccag ccgtacctgt cggccattgg atctacagcg
     6721 gtgtaacgac tcatgtattc ctgtcccgtt tcgaactggt ccaccacatc gtagagtccg
     6781 cagccctctc tgtcccttac cctgtcgaaa tacgcgagat actttttcag tggttcgtag
     6841 atgcgctcta tgaaggatct gtccggatgt acccggtgca gttccatcac cgccctaccc
     6901 cagtcggcgt ggtaaaaacc gttctcctgc aactggttga cgtatatgtg cccggcgaag
     6961 gagccatcct ccctctggtt ttctatgaaa ttcaagagac ttccccttgc cacctccggg
     7021 ctggccgacc atctcgtctc gagcatgtgg cactgtgcgc tgtaggttat gaaggcccgg
     7081 aaataatcca gtccctcggc tatggccggg tgcttcatgt aggcttcgtc cacttcgccg
     7141 gtgaagagtt tgagaccgta ccagcggtaa tagtagtatt tctggaaata gggttccccg
     7201 atcgaaaagc gtgggaggaa tctgaagtac gactcccagt ttctccggct ctcctcgacg
     7261 gggtcgcacc cgagagatct tccggctgcc ctcacgactt cctccacacc cggggccgcg
     7321 cccagtccgc agttgaaggt cgtctcgctt ccgggagcga gtttcacttt cctgtgcagc
     7381 cctatgtaca gcaagccctc ccggttgatg ccgttcgtat cgattctctc gatcaatcca
     7441 tctctttcga gtttctcata gaacggggtc aatctgaaat tcgggaggtt tcccgttctc
     7501 tccgagaagg atacgttgtg gctttcctta ccttccagag ccagaatcac gtgagcggga
     7561 taaggtcttc tcctgggctt gacaagtctc ttcttgaaaa cgataccgct gccgaccgca
     7621 tctattatct gcgtatcgac ctcgctgtcg ctatcggagc actgctgggc tgtccagacg
     7681 actatatcca gctctttcat ttccttcgag tcgttcttca gggttaccct ggaggccaga
     7741 aaatcctcct tcagcagggc cttctcttcc agcatttcca gatcgcctgc acggaagagg
     7801 ctgctcaaag aggcggggtt ccagagtctg tcgatacaga tgaaatcgac cggaactccc
     7861 cggtcgatta cgctgatcgt gtacccgggg tcgatcctca aatcgaaata actgacaaaa
     7921 tcccaggcac cggggcgatc caggtgcaac gggaaggggg gcgtccacag cagtctgttg
     7981 ccgccgccca ggtaccactt gtcttttctc tccagcatct tcaggatgtc aatctccatc
     8041 tctcttcact cccgtgattg aatgctgcat ctgcgatttc ggttcgtaac cggtgaggtc
     8101 gataatgtct ttcctgtcaa ggcttattat ccggtcgtag atctccagcg ccgcatcaag
     8161 attgcgctcc cttatcgcct cgtaaagatc cctgtgtagt ggcagtccag cgtcgccgaa
     8221 agtatccagt ccgagggggg atttccagag cacttcaagc ccgtcgaaga ccacatcaaa
     8281 gacttccttt aggaagcggt tgtgggagat atcgaataac ttcttgtgaa agcaggcgtc
     8341 ggtatcgccc aggttctccc tggtggatat cgccctggac agcctgtcca tacagtgcga
     8401 taactcctca agatcggccg gactcgcgtg agaaacggca agcgctatcg ccgatctctc
     8461 gagcgtgctt cgcagttcga tcagatctag aatgtctctc gcgctgtcgg tctctatttc
     8521 gaagttcacg ttgaacagta tcttcaggct ggttctcttc agaacgaaag tgccgctgcc
     8581 gtgaacggtt ctggtaactc cttcgtactc cagtaccctc agcgcttccc ttattatggt
     8641 cctgctgaca cccagcctgc tggaaagctc cacctcgccg ggcaatctct ccagatcttc
     8701 gaggactatc atctttttca gtctgttcac aacatccgta gaagtgcgtt tcttcgtctt
     8761 catagccatt ttctaccacc tgtatttgat tagttgtctg ctcactagac aactctatca
     8821 tttcgattct accttcgaaa taacctacat cgaaggtcgg ctatggacat ttcggggtgt
     8881 atacacggaa tctgaaacgt atattccaga ataagtctac aaaacgaaag gtgatgaatt
     8941 tacttgttga caagttgggg agaaatcgta tattacccgt acaggaaaaa acgagattca
     9001 aaagaaaggc tcatcacgaa gcgatagcgg cttcaggaga tcccgcactg gagcaagggg
     9061 ctgacaatcc cgggtgtttg cttcgaaagc tgtcattacg gcagttgctg ctggtaattg
     9121 tattccctga gaaattcgat tgctgctttt tcattcagcg gttcgctgat caagtaccct
     9181 gtattctgtc gcaattccat ctctccagag atgggttttc ttatatagtc cgtggcgccg
     9241 agctccaccc cttcgatttc attgtctatc tcatcagggc tggtcagtat tattacacgc
     9301 agttccctat acctatcatc tgacctcaac ccagcaagaa aatcgaatcc gttcatttca
     9361 tcccctttag attgcgagca atcaccggtg attacgctaa agaaaactac cggatttgta
     9421 ttctttgtat ctaccaacaa ttgtttaagt catagtatta atcggcggag acccccctgc
     9481 cctgaaatgt tcttattcag ggctcccatt caggaatcct aaatgtgacc ccgttcttta
     9541 tatacgggac cttgcccttc cgctctttcg aaggacggct cctgctgctc ctcatggacc
     9601 aggaatcagg gaatctggcg tgatattaat ttttaaagga gttttttatc ctgattttca
     9661 cgccgggttt ttctctgcac tcgaccggaa gaagcagcac gacatctttg ccctccattt
     9721 tcgcgttaca caagacgtgt ttcgagtcgt aggggccgca gtgttccagc gctgcatagc
     9781 ttccgttctc gtcgatctcg acatcctggg gtctgaagac tgcgccgccc actctgttga
     9841 tacccatggt cgaaaagagt tccatcacga agtgagaggc gggactggca taaacctccc
     9901 ggggtccgcc gctctggacg aagactccgt cattcatgac gtaaactcga tcggttatcg
     9961 ccagcgcatc ttccggatcg ttgagaacta tcaggaccga ctctcctatg gccatcagca
    10021 tgcgttttat catcgccctc agttccgtcc tgatcttttt gtcgagcctt tcgaagggtt
    10081 cgtccatgag gatcaggttg aactttttca ccgtctcgcg accgatcgca gtcagttttt
    10141 tcataccggc cggcagttcc ttcggccagc ggtcgaggta gtcggccagg ccgtcgagct
    10201 cggccgcctt tttctgtact ttctcttcgg tctgggggtc cttgcggatc ttcaggggga
    10261 aggctatgtt gttgtgtgtg tcgaagtgcg gaaagagcgc gttatcctgg aagacgaagg
    10321 ccaggcccct caggtggggc tcgagatagg tgacatcctt tccatctatg aagacctgtc
    10381 cggccacggg ctcctgaaga ccggctatgg ttcttaggag gagcgtctta ccgcacccgg
    10441 aaggtcctat gaggcccact atctcttttc tgcccacgtt cagatctacc ggccccagtt
    10501 cgaaagtgcc gattctggct ttcaggttca cgactttgag agtgtccatc agatcacctc
    10561 ccagagagat tatagtccgc cggtagtgaa tttcgataag aaagagagtc ttttccacag
    10621 gcggctaaga gtgtcgccct ataaagacgc gcgaaacctt tgcgggctat tcatagggag
    10681 agatggtctc taattgttct tcatacggcg cggaaatccg gttataatag taaaaacaat
    10741 tcagggagct gatagcgtga aggtaagtct tctatcgaaa gccgaaagtt acagggaaga
    10801 tctggccgat tttttgagcg atctggtgag cataaggagc ttctcggccg gtgaacgcga
    10861 agtgatcgaa aggataagac gggaaatggt taaggtcggc ttcgacgagg tgatagtcga
    10921 c
//
LOCUS       DAOOLY010000055        10845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6865_length_10845_cov_5.121501, whole genome shotgun sequence.
ACCESSION   DAOOLY010000055 DAOOLY010000000
VERSION     DAOOLY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6865_length_10845_cov_5.121501"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..130
                     /locus_tag="PLD15_04295"
     CDS             11..130
                     /locus_tag="PLD15_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB63155.1"
                     /translation="MGLCGVSPVMMINDEAYGNLTKERVSEIIDSIVSRERGE"
     gene            133..1752
                     /gene="nuoF"
                     /locus_tag="PLD15_04300"
     CDS             133..1752
                     /gene="nuoF"
                     /locus_tag="PLD15_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPB63156.1"
                     /translation="MSKPITVLVSIDSNSLLLGARHYKNYLASLVEKYNLGSIVDVLE
                     TGSFGDYGKGVLFLVLPENTVYSVKSEVDVEKIVLEHLLKGRIARELVVEDFSTEGPV
                     EAKSTTIEERVVLRNAGSIDPRNIEEYIALNGYQGLAKAFRMEPRAVIEEIKKSGLRG
                     RGGAGFPTGLKWEFTQKVDSPDKYVICNADEGEPGTFKDRLIMEGDPHSVIEGMVVAG
                     YAVGASKGYIYIRGEYFESVASLRRAISQAYEYGLLGASILGTDFSFDLSVRLGAGAY
                     VCGEETALIESIEGKSGRPRLKPPYPPVSGLYNKPTVVNNVETFACVPPIIDKGANWF
                     KAIGTKSSAGTKVYSLCGNLVKRGIVEVPMGTTVADLVYKFGGGIPDGRAVKMVQTGG
                     AAGTFIKPEELGAPLDFDSFKNSGASLGSGVILAIDETHCAVDVAKNLMEFFAHESCG
                     KCSPCREGTHIIVHILNEFSKGRGTRELFDQLYDVADTMEDSSFCGLGQAVPVPLRSI
                     LDRFKDEFLAHVDTLQCPVGICKFDKKKKKR"
     gene            1823..2626
                     /locus_tag="PLD15_04305"
     CDS             1823..2626
                     /locus_tag="PLD15_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPB63157.1"
                     /translation="MDKGKRLDLIVLLMLLTSIVVISGLLVGFGNRLSPKKVASLAIL
                     YNAGLGANREDFLNNSSYTYDDRVVSIYEYFAGKSDSKPVLSSAIKMHSVDDTELFST
                     NPAVDKLISSRPPRENSSLKNKLLSLVKSNKQLDSKGDEFKIKLGEAIYRAIMDFTGV
                     KVNIIVGGKVKTLDLSRLDPSIVVSIMIVESSLNPYALMEERSLNPSFSPYVYSRGLM
                     QIYELTLWTLNSWLKESQINVKPEGLWSIRDNIFLGMVYLSYANEMLEE"
     gene            2630..4117
                     /gene="aglB"
                     /locus_tag="PLD15_04310"
     CDS             2630..4117
                     /gene="aglB"
                     /locus_tag="PLD15_04310"
                     /EC_number="3.2.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047798 - cyclomaltodextrinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase"
                     /protein_id="HPB63158.1"
                     /translation="MDDWQRDAIIYQIFPERFNIGMARDVFEKREMGHYRLPEQRVRA
                     WNERPHASRDGGHQYDFWGGDLRGIIEKLDYIKDLGVNTIYMTPVFWGHTNHKYDTLD
                     YFTIDPDFGTEEDMKDLLKIAHSKGLRVILDGVFNHAGMAGKWFNGLNIFGQPGAFQG
                     NDEKGNFFVRVNGQFRGWMDSRNLLELNLENPELAEIIYRSEESVMKRWLRLGMDGWR
                     LDVAFDIGPDILSLMSDSVKSVNPRACIVGEIWNYPKGWREKAKLDGLMNYYYRSLIW
                     DSLRGSLKGEMAAVILGDCIHDCGLDYMSRSWNILSSHDVPRLRNELSSFDKIKAALV
                     LQYTLPGIPMIYYGEEIEMEGGHDPENRGPMEWEKVSSPPRTYHLYRRLNETRNSKTA
                     LRKGEFTVLRAGDPSLITFKRHTESIDELIVSVINTSERNVRTRVYLQEGFLMNGTAM
                     RDILTGKTLSTSFGSLEANIEPGEALILEPVIDRTVNRYSPYKRI"
     gene            4114..5028
                     /locus_tag="PLD15_04315"
     CDS             4114..5028
                     /locus_tag="PLD15_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB63159.1"
                     /translation="MKTSRGKSLGLIVLAITSMMWGLSYIFTKVAVESLPPMFLAVLR
                     FSLAVAVLFPIVGRRRGSFGRVRHRFAALAGLLGITTYFFFENYGLSLTNASDASLIV
                     STAPILTIILYDVLRRRFDYLEYIGGGIAFVGLGLIIYSGSFSSGSSVAGNLLSFGAA
                     LSWAGYTYFYERIRNSSIWTTMEIMLWGLVFSLPFAFVEIFLLGKSVSFSSGALAGVV
                     YLGLFASALGYILWNMGINLWGGKAATLWVYTIPVFTVMADVLFLKNVPSWLFFVGSA
                     FVGIGMITVIVREFRSMVSESGKETANR"
     gene            complement(5018..8551)
                     /gene="smc"
                     /locus_tag="PLD15_04320"
     CDS             complement(5018..8551)
                     /gene="smc"
                     /locus_tag="PLD15_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPB63160.1"
                     /translation="MLRLSSIYIKGFKSFAMPTKFDVSAGITAVVGPNGSGKSNVVDA
                     IRWLFGEQSMKNIRADNREDVIFNGSEKFPPSNSAEVRLVFESQEGSFSIAREVSRDG
                     LSQYRVNDRPGRLKDIKELFQGTGVGMDIYSIVGQGQVDKVVSASPYELRALIEEAAG
                     TAIYKERKKEALGKLASTEENLNRLEDIIFELGKQRKSLYLKAKRAEKYLEYAQKEKE
                     LKGLFFGNIARIEEETLFTHRGEFERIREELKELQKKLVEAESNWSTLRSEFSEVDKE
                     IEGFTQLLEEYKKRQNDLLELKEIYARRLGERENRLIEVSTRIDSLKNEIDELEKRKD
                     EIKLIIDSLNQQVYEEERLLTSLEEKRDSLVKNYSSREREWLKLQESFDSTQKRLSKI
                     ENELERLENNREDTTKRLRLIQTQLQSKRERFETLKEEIESLARQGKESSDRQKEVEA
                     NIKAAKDRMAELDSSLEKARESLTQDQNELKRSQMERTLLEKQQHEYQGFSRTVREVF
                     NRRDSFNGLRDVVANIVDVSQSFETAITILLGSRMQDIVVDDAITAKRIVEFLKTYKI
                     GRATLLPIDMIEGSFNRYSKIENHPGFIGYAAKLIEVPEGFEKLPPYLFGNSVVTRNL
                     DDAIEMRRELGFSGRMVSIDGQLVSGGGSITGGFIGDEARTDLLSRKRRIAELLEREV
                     ELEKGIALREKEILRLKDDITELRGYYKVLQEELNELASKGAAINRMIHELLKSTQEV
                     EDEIGELVKLETEYSRRLQENHLKKESLVNEQSALKIEKARLEESVEAESEELKRQKK
                     VLEELQDSIVDSKLRLSTLYEKQEQYSKEHASILEKKRSDQENVNLLSREMQEVEEET
                     ERLRKQVADQEREIASVKRETEDLFSSIRYQREGKEQRLNALQEAEGVINELKEKREK
                     KRDRSHQLEMLIQESQMKLTRVREEMENSGENTADVPVLSEEKLREVKVELDDYENKL
                     KFLGSVDLDAIEEYGVVDREYQELNDQKADLEDARQKLIELIEKTDTKAKTIFMETFD
                     RINKNFAAYIDEIFEGGEGDIKIISGDDLLETGLEITVRRPGRKIQKLQLLSGGEKAL
                     VGIALIFSLLSIKPSPFYVLDEVDAPLDDFNAERFRVLLKKHAFDTQFLVVTHNKLVM
                     EVANVLHGVTMTDGLSRVIPVELHSVESITG"
     gene            complement(8538..9551)
                     /locus_tag="PLD15_04325"
     CDS             complement(8538..9551)
                     /locus_tag="PLD15_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63161.1"
                     /translation="MNIIPVFLPHEGCESHCTFCNEYSATGLKKLPVREEILSTVQRY
                     LLYFVDKNNVELAFYGGTFTGSKRQKIYLDIGLELFERKVIKGIRFSTSPGQFNREKA
                     LLLRNYPISLVELGVQSFDPLVLKLSNRDHGVKEIYSATKVLKDFSIPFGLHLMTGLP
                     GDNHAKDLISSLEAVRTGAVSVRLHPLVILKDSLLAQDFVRGLFSPPGLEESLEILWK
                     MYLIINGAGVVVNRIGVCLYGNEIKNVVAGPYHPALGELVRNRLMLEVLREHHRLGGS
                     DHIALDKSHKGDFTGHRRYVIVTASNEGIIVQFRENGLRLDVESYLNSIRERLLGGIY
                     AQT"
     gene            9824..10762
                     /locus_tag="PLD15_04330"
     CDS             9824..10762
                     /locus_tag="PLD15_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase A"
                     /protein_id="HPB63162.1"
                     /translation="MERTEKLSDRIMEHLRDPFVRAAVTVKSKVIAESGNFLRGKGFV
                     ELLPTIISPVTDPLNHEVHNAKFRYYDQEYKLMQSMIFHKQLACTAFDRIFIISPNVR
                     LETSEKASSGRHLFEFTQIDLEMRDSRREDVMEVMEGLIVNVITSVKRDCGQALDYLG
                     SDPAVPSRPFPKVKYLDAYDQYGKDFETILSKSFTEPFWLIDIPIWEREFYDRLSDDG
                     KTLVDMDLILPLGFGEALSGGEREWEYRRIVKRMEFKGSDPKELSWYMEIAEDGNLVP
                     TAGCGFGVERLTRYLCSLDHVAKTRLFPKVPGQSCI"
BASE COUNT         2551 a         2702 c         2674 g         2918 t
ORIGIN      
        1 gaccagctgc ctgggacttt gcggagtatc tcccgtaatg atgataaacg acgaagctta
       61 tggaaatctc acaaaagaga gagtttcgga gattatcgat tcaatcgttt ccagagagag
      121 aggtgagtga gtatgagcaa acctattacc gttctggtat caatcgactc gaacagcctt
      181 cttctcggtg cgcgccatta caaaaactat ctcgcctccc tggtggaaaa atacaacctg
      241 ggatcgatcg tagatgttct cgagacgggt tctttcggag attacggaaa gggtgtactg
      301 tttctggttc tacccgagaa caccgtttac tcggtcaaat ccgaggtcga tgttgaaaag
      361 atcgttctcg aacatctact caaaggtagg atcgcccggg aactggtcgt tgaagatttc
      421 agcaccgaag gaccggtcga agcaaaaagc accacaatcg aggaacgcgt agttctgcgc
      481 aatgcaggtt caatcgatcc aaggaacata gaagagtaca tagctctcaa cggctaccag
      541 ggactggcca aggctttcag aatggagccc cgggccgtta tcgaagagat caagaagagt
      601 ggattgcgcg gccgtggtgg tgcgggtttt ccgactggtt tgaaatggga gttcacccag
      661 aaagtagatt cgcctgataa gtacgtgatt tgcaacgccg acgagggtga gcccggtact
      721 ttcaaagacc gcctgataat ggagggcgat ccccactctg tcattgaagg tatggtcgta
      781 gcaggttatg ctgtcggtgc cagcaaggga tatatatata taaggggaga atacttcgaa
      841 tccgttgcca gcctcagaag agcgatcagc caggcgtacg agtacggtct tctcggggcc
      901 tccatactgg gcaccgattt ttccttcgac ctctcagtga gattgggagc cggagcctac
      961 gtctgtggag aggaaacggc gctgatagaa tcgatcgagg gcaaatcggg ccgccctaga
     1021 ttgaaaccgc cttacccccc ggttagtggt ctgtacaaca aacccactgt ggtcaacaac
     1081 gtcgaaacct tcgcctgtgt tcctccgata atagacaaag gtgcgaactg gttcaaagcg
     1141 atcggcacga agtcttcggc tggtacgaaa gtttactccc tttgtggcaa tctggtcaag
     1201 agaggcatag tcgaggttcc catgggcacc acggtggccg atctcgttta taagttcggc
     1261 ggcggcattc ccgatgggag agccgtgaag atggttcaga ctgggggcgc ggccggtacg
     1321 tttataaagc ccgaagaact gggtgcacct ttggatttcg attccttcaa aaattccggt
     1381 gcgtcgctcg gttcgggagt aattctggct atcgacgaga cccactgtgc ggtcgatgtg
     1441 gcgaaaaacc tgatggaatt cttcgcccat gaatcctgcg gtaagtgttc accctgtcgc
     1501 gaaggtacac acataatcgt ccatatattg aacgagttct ccaaaggtag aggaaccagg
     1561 gagctcttcg atcaactata cgatgtcgcc gatacgatgg aagactcttc cttctgtgga
     1621 ttgggtcagg ccgttccagt accgctgagg tcgattctcg accggttcaa ggatgaattt
     1681 ttggcgcatg ttgacacact ccagtgtccg gtgggcatct gtaagttcga taaaaagaag
     1741 aagaagagat aattgagagt tacaaaatta tcgagccggg gcaacccggc ttttctctga
     1801 tataataacc cagaggtgga aaatggataa aggaaagaga ctggatctga tagtccttct
     1861 catgttgctg acttcaatcg ttgttatatc aggtcttctg gttggttttg gaaaccggtt
     1921 gagcccgaaa aaggtggcct ccctggcgat cctttacaat gccggtctgg gtgccaacag
     1981 ggaagatttt ctcaacaact cttcctacac ttacgacgac agagtggttt cgatttacga
     2041 atatttcgcc ggcaagagcg attcaaaacc agttctgagc tctgcaataa agatgcatag
     2101 tgtggacgat acagaactct tttctaccaa ccccgccgta gataaactga tctcctccag
     2161 acctcctcgg gagaacagct ccctcaagaa caaactgctg agtctggtca agtctaacaa
     2221 gcagctggat tcgaagggcg atgagttcaa aatcaaatta ggagaggcca tttacagagc
     2281 tatcatggat ttcacgggtg tgaaagtaaa tataatagta ggcgggaaag tgaaaacgct
     2341 cgatctctcc agattggatc cttcgatagt cgtctcgatt atgattgtgg agtcctctct
     2401 caacccttac gcgctgatgg aagagagaag tctcaaccct tctttctctc cgtacgttta
     2461 ctctcgcgga ttgatgcaga tctacgaact aactctctgg acgctgaact catggctgaa
     2521 agaatctcag ataaacgtta aacccgaagg actctggtcg atcagggaca atatttttct
     2581 tgggatggtt tatctttcgt acgcgaatga aatgctggag gaatgatcta tggacgactg
     2641 gcagagggat gcgataatat accagatctt ccccgagaga ttcaatatcg gcatggcacg
     2701 cgacgttttc gagaagagag agatgggcca ctacagactt cccgaacagc gggttcgcgc
     2761 ttggaacgag aggccacacg cctcgcgcga cggcggtcac cagtacgatt tttggggagg
     2821 agacctgagg ggcattatag agaaactcga ttacataaag gatcttggag taaatacgat
     2881 ctacatgact ccggtattct ggggtcacac caaccacaaa tacgatactc tcgactattt
     2941 cacgatcgat cccgatttcg ggactgaaga agacatgaaa gatctcttga aaatcgctca
     3001 ttcgaaaggg ttgagagtaa ttctggacgg tgtgttcaac cacgccggaa tggccggcaa
     3061 atggttcaac ggccttaaca ttttcggcca gcccggcgcg tttcagggca acgatgagaa
     3121 aggaaatttc tttgtgcggg ttaacgggca gtttaggggc tggatggatt cgagaaatct
     3181 actcgaactc aatctcgaaa acccagaact ggccgagatc atttatagat cagaagaatc
     3241 tgtcatgaaa cgctggctga gattaggtat ggatggctgg cgactggatg ttgctttcga
     3301 catcggcccg gatattctat cgctaatgtc ggacagtgtg aagagtgtta atccgcgggc
     3361 ctgtatcgtc ggagagatct ggaactatcc taaaggctgg agagagaagg cgaaactcga
     3421 cggtctgatg aactattact acaggtctct gatctgggac tctcttcggg gttcgttgaa
     3481 aggtgagatg gccgccgtga ttcttggcga ttgtatccac gactgcgggc tcgattatat
     3541 gtccagatcg tggaacatac tgtcctctca cgacgtaccg agactcagga atgaactcag
     3601 ctccttcgat aaaatcaaag ctgcgctcgt cctgcagtac acactgccgg gcattccgat
     3661 gatatactac ggcgaagaaa tcgaaatgga gggcggtcac gatccggaaa acaggggtcc
     3721 tatggagtgg gagaaggttt ctagcccgcc ccgtacctat catctctaca gaagattaaa
     3781 cgaaacaaga aatagcaaaa ccgcactccg gaagggcgaa ttcacagtcc tgagagccgg
     3841 tgatccttct ctgataactt tcaagagaca taccgaaagc atcgacgagt tgatcgtttc
     3901 cgtaattaac acatctgagc gtaacgtcag gaccagggtc tatttacagg aaggcttcct
     3961 tatgaacgga actgctatga gggatatact gaccggaaag acgctttcaa cttccttcgg
     4021 ttcgctcgaa gcgaacattg aaccgggaga agccctgata ctcgaacccg taatagacag
     4081 gactgtgaac cggtactcac cttacaagag aatatgaaaa cttcaagggg gaaaagtctc
     4141 gggctgatcg ttctggcgat cacttcgatg atgtggggtc tttcgtatat cttcacgaag
     4201 gttgcggtgg aaagtctgcc gccgatgttt ctggcagttc tgcgattttc cctcgctgtc
     4261 gccgttctct ttcccattgt agggagacga aggggctcgt tcgggagagt aagacacaga
     4321 tttgcagcgc tggccggatt gctgggaata actacctact tctttttcga gaactacggt
     4381 ctcagcctca ccaacgcttc cgacgcctcg ctgatagtct cgacggctcc gatcctgacg
     4441 ataatacttt acgacgttct gcgcagaaga ttcgactatt tagaatacat cggtggcggt
     4501 atagcttttg tgggcctggg gctgataatc tattcgggaa gcttctctag cggttcaagc
     4561 gttgctggga acctactctc cttcggagcg gctctctcct gggccggata cacgtacttt
     4621 tacgaaagga taagaaacag ttcgatttgg accaccatgg agataatgtt atggggactg
     4681 gtcttttcct tgccgttcgc ctttgtagag atcttccttc tcggaaagtc cgtctccttt
     4741 tcctcaggag ctctggccgg ggttgtctat ctgggactct tcgcctccgc gcttggctat
     4801 atcctctgga acatgggaat aaacctctgg ggaggcaagg cggcgacgct ctgggtctat
     4861 acgattccgg tcttcactgt tatggcggat gtgttgtttc ttaaaaatgt accttcgtgg
     4921 ctgtttttcg tcggttcggc cttcgttggg ataggcatga taacggttat tgtcagagag
     4981 ttcagatcga tggtctccga aagcggtaaa gaaactgcta accggtgatc gactccaccg
     5041 agtgcagctc gaccggtata actctggaga ggccgtcggt catcgttacc ccgtgcagca
     5101 cgttggccac ttccatgact agcttgttgt gtgtgacaac cagaaactgt gtgtcgaagg
     5161 cgtgcttttt cagaagcacc ctgaacctct ctgcattgaa atcgtcgagc ggcgcatcga
     5221 cttcatcgag tacataaaag ggactgggct ttatgctcag tagcgaaaaa atcagcgcga
     5281 ttcccacgag cgctttttct cctcccgaga ggagctgcaa tttctggatc tttctaccag
     5341 ggcgtcgcac ggtgatctcg agtccggttt cgagaagatc gtccccggag attatcttga
     5401 tatcgccctc tcccccctcg aagatctcgt ctatataagc cgcgaagttc ttgtttattc
     5461 tgtcgaaggt ttccatgaaa attgtcttcg ccttcgtatc tgtcttttcg atcagttcta
     5521 tcaacttctg tctggcatct tccagatcgg ccttctgatc gttgagttcc tggtactccc
     5581 tgtcaaccac tccgtattct tctatcgcat cgagatcaac gctgcccagg aacttcaact
     5641 tgttttcgta atcgtccagc tcgaccttaa cctcccgcag tttttcttcc gaaagaacgg
     5701 gaacatcggc agtattttct ccagagttct ccatctcttc tctcactctg gtcagtttca
     5761 tctggctttc ctgtatcagc atctccaatt gatgggagcg gtctctcttc ttctcgcgct
     5821 tctccttcag ttcgttgatt acaccttcgg cttcctgaag agcgttcaat ctctgttcct
     5881 taccctctct ctggtatctt atgcttgaga agagatcttc ggtttctctc ttcaccgatg
     5941 cgatctctct ctcctgatcg gcgacctgct ttctcaacct ctcggtctcc tcctcgacct
     6001 cttgcatctc tctggaaaga aggttgacgt tttcttgatc ggagcgtttc ttctccagta
     6061 tagaggcgtg ttccttagaa tactgctcct gcttttcata taaagtcgat agtcttagct
     6121 tcgaatccac gatgctatcc tggagctctt caagaacctt cttctgtctc ttcaattctt
     6181 ctgactcggc ctcaacgctc tcttctagtc tcgccttctc tattttcagg gcagattgct
     6241 cgttgaccag agactccttc ttgagatgat tttcctgtaa tctgcgtgag tactcggttt
     6301 ccagtttaac cagttctcct atttcatcct cgacttcctg tgtcgatttc agaagttcgt
     6361 ggatcattcg atttatggca gctcctttcg aggccagctc gttgagttcc tcctggagca
     6421 ctttgtagta gcccctcagt tcggttatat cgtctttcag gcgcagtatc tctttctctc
     6481 tcaaagcgat gcccttttcc agctcgactt ctctttccaa aagctcggcg atccttctct
     6541 ttctagagag aagatccgtt ctagcttcgt cgccgatgaa gccaccggtg atcgagccac
     6601 cgccgctaac gagctgacca tcgatcgaga ccatacgtcc ggagaagcca agctcccttc
     6661 tcatctcgat cgcatcgtca aggttcctag taaccacgga atttccgaag agatacggtg
     6721 gcaacttctc gaaaccctcc ggtacttcga tcaatttggc tgcgtagcct atgaagccag
     6781 gatggttctc tatcttcgag taacgattga aactgccctc gatcatgtcg atcggcagga
     6841 gagtggccct tccgattttg taggtcttca ggaactccac tatccgcttg gctgtgatgg
     6901 cgtcgtcaac gactatatcc tgcatcctcg aaccaagcag gatcgttatg gcagtctcga
     6961 aggactgtga aacatcgacg atgttggcga cgacatctct caacccgttg aagctatccc
     7021 gtctgttgaa tacctctctc acagttcttg agaagccctg gtactcatgt tgctgcttct
     7081 ccaggagggt cctttccatc tgcgaacgct tgagttcatt ctggtcctga gtcagagact
     7141 ctctggcctt ttccagggac gaatccagct cggccattct gtctttggcc gcctttatat
     7201 tggcttcgac ttccttctgc ctgtccgagc tctcctttcc ctgtctggcg aggctttcta
     7261 tctcctcttt tagagtttca aaccgctctc ttttggattg aagttgtgtc tggatcaatc
     7321 tgagtcgctt tgtagtatct tcacggttgt tttcgagacg ctccagctcg ttctcgatct
     7381 tagacagcct tttctgagtt gaatcgaagc tctcctgtaa tttcagccac tctcgctcgc
     7441 gcgagctata gttcttgaca agcgagtcgc gtttctcctc gagagatgta agcagtctct
     7501 cctcttcata gacttgctgg ttcagactgt cgatgatcag ctttatctcg tcttttctct
     7561 tctccagttc atcgatttcg tttttgaggg aatcgattct ggtggatact tctatcaggc
     7621 ggttttctct ctccccgagc ctcctggcgt atatttcttt cagctccagc aggtcgttct
     7681 gtcttttttt gtactcctca agaagttgcg tgaagccttc gatctcttta tctacttcgg
     7741 agaactcgct cctgagggtt gaccagttgg attcggcctc caccagtttt ttttggagct
     7801 ctttcaactc ttctctgatt ctctcgaact caccccgatg agtaaaaagc gtctcttctt
     7861 cgattctggc gatatttccg aaaaagaggc ctttcaactc cttctctttc tgtgcgtatt
     7921 ccaggtattt ttcggctctt tttgccttca agtagagaga ctttctctgt ttgcccagtt
     7981 cgaagataat atcttcaagc ctgttgaggt tctcctcagt ggaggcgagt tttccaaggg
     8041 cttccttctt tctttccttg tagatcgccg ttccggccgc ctcttcgatc aaggctctta
     8101 actcgtaagg cgaggcactt acgaccttgt ccacctggcc ctggccaacg atggagtaaa
     8161 tatccatccc gactccggtt ccctggaaga gttcctttat gtccttcagc ctacccggtc
     8221 tgtcgttcac tctgtactgt gaaagcccat ctctggaaac ctcgcgggct atggagaaac
     8281 tcccctcttg cgattcgaag accagtctca cctcggcaga gttggaaggc ggaaacttct
     8341 ccgaaccgtt gaagatcacg tcctccctgt tgtcagccct gatgtttttc atcgactgct
     8401 cgccgaaaag ccagcgtatc gcgtcaacga cgttggactt gccgcttccg ttgggaccga
     8461 ccactgccgt tattcccgca gatacatcga atttcgtggg catcgcgaaa cttttgaatc
     8521 cctttatata gatcgagcta agtctgagca tatatacccc ccagaagcct ctctctgatt
     8581 gaattcagat agctttcaac atcgaggcgg agaccgtttt cccggaactg aactattata
     8641 ccctcgtttg aggcagtaac gatcacgtat ctcctgtgcc cggtgaaatc gcccttgtga
     8701 gatttgtcca gagcgatgtg atcgctccca cccaatctat ggtgttcgcg gagtacttcg
     8761 agcataagcc tgtttctaac aagttcaccc agtgccggat ggtaagggcc tgccaccacg
     8821 ttcttaattt cgtttccata gaggcacaca cctatcctgt taacgacaac acctgccccg
     8881 ttgattatca ggtacatctt ccaaagtatt tcgagcgatt cttccagacc gggtggcgag
     8941 aagagtcccc ggacgaagtc ctgtgcgagc agcgagtctt tcagaataac cagcggatgg
     9001 agtctcacgc tgactgcacc tgtacggaca gcttccagag aggagatcag atccttggcg
     9061 tgattgtctc cgggcagacc ggtcattagg tgaagaccga agggtatgga gaaatctttt
     9121 agaactttag ttgccgaata gatctctttc acaccgtggt cacggttgct cagtttcaga
     9181 accaggggat cgaaagactg cactcccaat tcgaccagac tgatcggata atttcgcaga
     9241 agaagggctt tttcccggtt gaactgcccc ggtgaggtag agaagcgtat cccttttatc
     9301 acctttcttt caaaaagttc cagacctata tccaggtata tcttctgcct tttggaaccg
     9361 gtgaacgtcc cgccatagaa agctagctcc acgttgttct tgtcaacaaa atacaagaga
     9421 tacctctgta cggtcgaaag gatctcttct ctgacaggga gtttcttcag accagtggcc
     9481 gaatattcat tgcaaaaagt acagtggctt tcgcagccct cgtggggtag aaaaactgga
     9541 ataatgttca tgtaagtatt ataaccgacc cggagaattg ggggaaagaa gacatttcaa
     9601 caaatcttaa tgaaaagact ttctatgtta ccttacagat ggtagaatct ttcaaacgcg
     9661 gaggtactga catgggtagg atcataacga aatttgagaa gttcatactc aataataata
     9721 actggccggg atcggtcggt taatctctgc gtactgagta atttagaaga ttaaaaggcc
     9781 gatggatttc catcggcctt attttttaaa aggggttgaa aatatggaga gaacggaaaa
     9841 actcagcgac aggataatgg agcacctcag ggacccgttc gttagggcgg ctgttacagt
     9901 caaatctaag gttattgcag aatcgggaaa tttcttgcgg ggaaagggat ttgtcgagct
     9961 gcttccaacg ataatttcgc cggtcacgga ccctctcaac cacgaagtcc acaacgccaa
    10021 gttccgatac tacgatcagg aatataaact gatgcagtcg atgattttcc acaagcaatt
    10081 agcctgtacg gccttcgaca ggatcttcat catctcgcca aacgtacgac tcgaaacatc
    10141 tgaaaaggct tcctcgggca gacacctctt cgagttcaca cagatagatc ttgaaatgcg
    10201 cgacagcagg agggaagatg ttatggaagt catggaagga ctgatcgtta acgtcatcac
    10261 ttccgtgaag agagattgcg gccaggcgct ggattatctg ggatccgacc cggccgtgcc
    10321 ttcccgcccc ttcccaaagg tgaagtatct ggatgcctac gaccagtacg gaaaggattt
    10381 cgaaacgatt ctctcaaaga gctttaccga acctttctgg cttatagata ttcccatctg
    10441 ggagagagag ttttatgatc ggctttcaga tgacggcaaa accctcgtgg atatggatct
    10501 tattctccct cttggctttg gcgaggctct atcgggcggt gaaagggagt gggagtaccg
    10561 gagaatcgtg aagcggatgg agttcaaggg gagcgaccct aaagagctgt cctggtacat
    10621 ggagatcgca gaagacggca acctggtacc gacggccggc tgtggtttcg gagtggagag
    10681 gttgacgaga tatctctgct ctctggatca cgttgcgaaa accagactat tccccaaagt
    10741 tcccggtcag agctgcatat gagaaaactc ccccgaaatt tcgggggagt cctggctcca
    10801 gcggagggac tcgaaccccc aaccgtccgg ttaacagccg gatgc
//
LOCUS       DAOOLY010000056        10830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6875_length_10830_cov_4.542274, whole genome shotgun sequence.
ACCESSION   DAOOLY010000056 DAOOLY010000000
VERSION     DAOOLY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6875_length_10830_cov_4.542274"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLD15_04335"
     CDS             complement(<1..243)
                     /locus_tag="PLD15_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HPB63163.1"
                     /translation="MDRLSEFIIRHRAAVVVFFGILGIFGAFLSSKLSIDSDLMKILP
                     EDDPVIRQYESFIEGNSSRDVTYIVIKSDDKSPGGID"
     gene            complement(346..1020)
                     /locus_tag="PLD15_04340"
     CDS             complement(346..1020)
                     /locus_tag="PLD15_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63164.1"
                     /translation="MKFRKATILFSLVALAFGLAAMIFTFYPAVQEVGSRKSSPDFSI
                     RNSIFPYLQDFKKHLESVQEFSSILKELEKEKAGAWLISLENGEKRFICPYTDTYNKL
                     RTLLLVETGSGGTRTVTTTKYFGNDVTRSEVVEYGGFYLDYVPSYSLIMESDYQRYLQ
                     EGYTFNKVYNANAFSLGRFVSDVFQGIGPVIVLKEGASLSERFFDVIERYSIPFMPVR
                     YFLRKA"
     gene            complement(1017..2255)
                     /locus_tag="PLD15_04345"
     CDS             complement(1017..2255)
                     /locus_tag="PLD15_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPB63165.1"
                     /translation="MKKNKLLKTVGVISVLVGVFFLGAFTSLSYENMLDKFSPMFEIL
                     TYIDRNYYDIEKVDYDNILDEVLTGTMRGLEDPFAWYFDPVETKENEIDTSSKYGGIG
                     SVVQYNIEFECLQVVSPMAGSPSERIGLRAGDLIITIDGTPVSETGYYESVNMLRGEP
                     GTTVILEVYREGFTETKFFEITREIIEIKSVKSEIIDFEGSSISYIQITGFNAPTYEE
                     FQNALDLTRDSQAYIFDLRNNPGGLLQSVLNISSLILPKGQRVITIRYRDGQEEIYNS
                     WGSRYNSYFTNKPIVVLVNEGSASASEILTGALKDHDIATVIGTKTFGKAAVQTVYNL
                     SNGGEIWLPTAHYFTPNGDDIHLVGIEPNITVEATAVSLEDENRALTLSTAAVDIEND
                     AQLFAALEAIAQMLGVGVAN"
     gene            complement(2252..3028)
                     /locus_tag="PLD15_04350"
     CDS             complement(2252..3028)
                     /locus_tag="PLD15_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HPB63166.1"
                     /translation="MIRAGDRVVILLEDEETRLFAEVREGEVLTTHYGNLIMSSLAGM
                     EYGSFVSLGFKGAYVLRPSFMDGILSLKRSTQIVYPKDIAFITLNLDVKDGDVVYEAG
                     TGTGVMTSVFSRLVGSSGRVITYEKRSDFVMRARENVAALGKIERVTFFPEDIERCDE
                     ENVADSFFLDVPDPLAVMDRAVSILKGGGRICVLCPTTNQVQESIRLLGELNIVDIQM
                     WEIMARNYKTNPDRLRPEDRMVGHTAFLIFGVKIKRRSRS"
     gene            complement(3029..3304)
                     /locus_tag="PLD15_04355"
     CDS             complement(3029..3304)
                     /locus_tag="PLD15_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPB63167.1"
                     /translation="MQGVDESTRKAVLNRLKRIEGQIRGLQKMVENDRVCGDILTQVS
                     AVNSALSRVSEMIIKGYARKCVIEANEAGKTEELDNLIENIIKLRGI"
     gene            complement(3334..4998)
                     /locus_tag="PLD15_04360"
     CDS             complement(3334..4998)
                     /locus_tag="PLD15_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPB63168.1"
                     /translation="MTKKTIRIKGIEESIPVSEGTTFQDLACRFSGRYGSPIMAVKSG
                     NSLFELCRLVGEEKEIEFVDLSSLDGIRIYTRGAIFILFMAVRELLGKVSLDVHHSRG
                     SGLICEIEGVVANRELLEKIEEKMREIAQENVPFIKDTIGKFEAIRIFREDGQIDKAL
                     LFKYRKKSTVNIYRCKDYLNYFYGYMPFSTGSVGYFSLIPYGNYFVLNLPSASEPHAL
                     PDFVDQPKISSIFLEHERWGSILGVKTIGELNEIISKGYGETREMINVAEALHEKKIA
                     GIADQIESRESVRVILVAGPSSSGKTTFSKRLMLQLRVLGMNPIQISLDDYFFDRKDT
                     PLDEKGNYDFESIYALNIELFNRQMGELLEGREVLLPKFDFLLGKSGFQEKAVSISHD
                     QPIIVEGIHGLNELLTASIPKERKFKIYVSALTQLNIDNMNRIPTTDVRLLRRIVRDN
                     RFRGHSALDTIRMWSSVRRGEDRYIFPFQEEADAMFNSALAYELGILKSFAEPLLVQI
                     DDSVPEFVEAKRLLRFIDYFLPITNLEDVPRTSIIKEFIGGSVFSY"
     gene            5063..5138
                     /locus_tag="PLD15_04365"
     tRNA            5063..5138
                     /locus_tag="PLD15_04365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5096..5098,aa:Gln,seq:ttg)
     gene            complement(5175..7220)
                     /locus_tag="PLD15_04370"
     CDS             complement(5175..7220)
                     /locus_tag="PLD15_04370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB63169.1"
                     /translation="MSAKKPRRNTNFMKSIQRPVVWAVAILFGIGIVWWTVAQYLGSG
                     SQNNTQNVDQTAKFEEMVGGLTRDGTPVTDSRYWINYSEYEISARDTLSNIRSQGYNL
                     DPYFEIENNPSEMGIRYEIFRSLVDQKTLLLYAVENNAVPTAEQIKSESDRIVNQYVG
                     DENTKAAIIYQYGSVEAFSEIVRDYVSTQLLSLNVQAKALPNIEEDFKQYVADNMVDL
                     KSEYEKVTASHILVTEEASALELKVKIEEGALSFSQAASEFSIDTGSAIDGGNLGEFG
                     HGQMVKEFEDAAFAATPGVITGPVISQFGYHLIDVATKTTFDTYEEFVKVAGYDTEYS
                     QYSNERFASWLSGYKEENKLSYAINDPDLKMYEKFANSRNDSESSQKLMTELQNEYFD
                     GEGNVLIGDSYLPMVIYTELADDRASELRNEVLDLEDLKGILTTVPASITSLSREEIA
                     AQLETTPSTDSIMRVYLSNSQRAYDYLDKYGLSELTDVEKLLSEKNEVYDRTNTYFEN
                     SVKFLYSAMPNSSQVINYMYRIDGNDPKIVYLYNESIYNRNIRPLLENPALLDSYLQY
                     YGQFFGAQAVSLLIDSPVQSVESDLNMKILNVTDVATDLKVSTLYLLVDIYEKLANIQ
                     EDEQMMRMYLTGEMGYLEMLQELYPEDAAVKSTIETVQFQISELATGTTVEATGTEN"
     gene            complement(7365..8969)
                     /locus_tag="PLD15_04375"
     CDS             complement(7365..8969)
                     /locus_tag="PLD15_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPB63170.1"
                     /translation="MRKGKFVLMLHAHLPFINHPDFPSFMEERWLFEAMTETYIPLVQ
                     AFNRLKNDGVHFRVSMSITPPLMEMFANADLQKKYLKYLDLSIELAEKEIERTISENP
                     ASNRLAKHYLKELEETKSVYIRCNGDLSGEFAQLQDSGNLEIVTCNATHGFLPLMTQY
                     PEAVNAQIGVGVDTYKRHMGKAPRGIWLAECGYVPGIDSYLREWGLSFFFVDSHGLWY
                     GDPAPRYGVYRPVMTPEGVFVFARDPESSQQVWSAEVGYPGDSRYREFYRDVGFDREE
                     DYIRPYIDPSGERCNTGIKYYRITGKNVSLNEKSYYDFEEAKKATREHAKDFAARKRE
                     QIKRLSEQFNGVSPVVVAPFDAELFGHWWYEGPIFIEELFRELNGDDTVVCATPPEVI
                     DSMGEVQCVTPNISTWGANGYNEVWLNGRNDWIYRHLDEGVRRMIELARKYEKTEDRL
                     KIRALNQMVRELLLAQSSDWAFIITTNTTVEYAVNRTKTHINRLLTLYGEIETGTIDV
                     ERLKTLEWIDGIFSSIDFRVYSRQVG"
     gene            complement(8974..9708)
                     /locus_tag="PLD15_04380"
     CDS             complement(8974..9708)
                     /locus_tag="PLD15_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HPB63171.1"
                     /translation="MKKEDIGSFLKTDPSIQEMRRYAKMLGVHLKRTMGKKDIARALA
                     KLSDKDVPVSSQTVVATPSESKRPSKPVNVDLPRTYFTDSITLHPVNPYWVYIKWDFS
                     KETRERLDELGNDIFVRVSDITNIIYDGKNANRYKEAKILSEEGNWYFQVDFPDADYI
                     AEIGYYAGELFVGFMKSKIARTPRNTPQFTDQETWVDLSKGSKKEVRVSVEAAETSLS
                     IRSSGPSDNPSSEEFIKSISKSRSGR"
     gene            complement(9845..>10830)
                     /gene="hisS"
                     /locus_tag="PLD15_04385"
     CDS             complement(9845..>10830)
                     /gene="hisS"
                     /locus_tag="PLD15_04385"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPB63172.1"
                     /translation="SFINLGSPVKLFYMGPMFRYEKPQAGRLRQFHQFGAEILGSASA
                     MADFEIIELACEFLKEVKINKTRLFINSIGCPSCRARYREALREYFRDKVDGMCEDCR
                     RRFDSNVLRLLDCKIDREQSENAPNILEYLDEECRKHFEDLKWYLEKARIDYEIDPHL
                     VRGLDYYSRTAFEIRSPSLGSQDQIVGGGRYDGLVEVIGGKSCPAVGFAIGMERIVML
                     LKAEKVNIPTDRNSEVAILATGREESLRGFEYARRLRKAGISTYVDVMERNMRNQMKH
                     AARIGAKISVIIGDEELEKDLVTVKIMMDGSQYQVDGDYMVDYIIDKLRDI"
BASE COUNT         2561 a         2826 c         2312 g         3131 t
ORIGIN      
        1 gtcgattccc ccgggagact tgtcgtcgct tttgataacg atataagtca catctctgga
       61 ggagttgccc tctatgaaac tctcatattg ccttatgact ggatcgtctt cgggaagtat
      121 cttcatcaga tcggaatcaa tactgagctt ggaagagagg aaagctccaa aaatccccag
      181 aattccaaaa aaaactacca cagccgcccg atgccgaatt atgaactcac tgagtctgtc
      241 cacgctatca ccactcatga atcgtcctgg ccgaattctc ttatatacta tcgtaaatcc
      301 acttcagagt acagtggcct cttttgattt gtatgctaaa gagtttcatg cctttctcaa
      361 gaagtatctc accggcatga aaggtatcga ataacgttcg attacatcga agaatcgctc
      421 cgaaagactc gcgccttcct taagcactat taccggtcct ataccctgga aaacatccga
      481 aacgaaccga cccaaactga aggcgttggc attgtaaact ttgttgaatg tatagccttc
      541 ttgaaggtat ctctggtaat cgctttccat tatcagcgag tacgacggga cgtaatccag
      601 ataaaagcca ccgtattcca caacttcgct tctggtaaca tcgttgccga agtacttcgt
      661 ggtagtcaca gttctggtgc caccgctacc ggtttcgacc agcaatagag tacggagttt
      721 gttgtaggta tcggtgtatg gacatatgaa cctcttttca ccgttctcca gtgatatcag
      781 ccaggctcca gctttttcct tctcgagctc tttcaatatc gagctgaatt cctgcacact
      841 ttcaagatgc tttttgaagt cctgtaagta aggaaatata ctgtttctga tagaaaagtc
      901 gggagaggac tttctgctcc cgacttcctg aaccgccgga taaaacgtga aaatcatagc
      961 cgcaagacca aatgccagcg caaccagtga aaagagtatg gtggccttcc taaatttcaa
     1021 ttggccacac caacccccag catctgagcg atcgcctcga gggccgcaaa tagctgcgcg
     1081 tcgttttcta tatctactgc cgcagttgac agggttagcg ccctgttttc gtcttccaga
     1141 gagaccgcag tcgcttcgac cgtaatgttc ggttctatgc ccacaagatg tatgtcgtct
     1201 ccgttcgggg tgaagtaatg ggccgtcggc aaccatatct cgccaccgtt ggagagattg
     1261 tacaccgtct gcacggccgc tttgccgaaa gtcttcgtac ctattaccgt cgctatatcg
     1321 tggtccttta gggctcctgt gagtatttcg gatgccgaag cgcttccctc gttaacaagc
     1381 accactatcg gtttatttgt gaaataactg ttgtaacgtg aaccccagga attgtagatt
     1441 tcctcctgtc cgtccctgta ccttatggtt ataacccgct gtcctttggg aagtatcaag
     1501 gaggaaatgt tcagaacgct ctgcaaaagg cctccgggat tgtttctaag gtcgaatatg
     1561 taggcctggc tatctctggt gagatcgagg gcattttgaa actcttcgta cgtgggcgcg
     1621 ttgaaccccg ttatctgtat gtatgaaata ctagatccct cgaaatcgat gatctcgctt
     1681 ttaacgctct tgatttcgat tatctccctg gtgatttcaa agaacttcgt ctcggtaaat
     1741 ccctccctgt aaacttcaag gatgactgtt gtgccgggct ctcccctcaa catgttaact
     1801 gattcataat agcccgtttc cgaaacgggt gtcccgtcta tcgttatgat caggtctccc
     1861 gctctgagtc cgatcctctc ggagggactt ccggccattg gtgaaacgac ctgcagacat
     1921 tcgaactcga tgttgtactg tactaccgat ccgatacccc cgtactttga actggtatct
     1981 atttcgtttt ccttggtctc caccggatcg aaataccagg cgaatggatc ttccagtccc
     2041 cgcatagtac ctgtaagcac ttcatccaga atgttgtcgt agtcaacctt ctcgatatcg
     2101 tagtaatttc tgtctatata ggtcaatatt tcgaacatcg gactgaactt atcgagcata
     2161 ttctcgtacg ataaagaggt aaaagctccc aaaaagaaga caccgacaag cacagagata
     2221 actccaacgg tttttaaaag tttattcttt ttcatgatct actcctcctc ttgatcttca
     2281 caccaaaaat aaggaatgcc gtatgcccca ccatacggtc ctcgggtctc aatctatcgg
     2341 gattagtttt ataatttctc gccattatct cccacatctg aatatcgacg atattaagct
     2401 ctcccagaag tcttatggat tcttgaactt gattggtcgt cgggcataga acgcagattc
     2461 taccgccgcc tttcagtatc gaaactgccc tgtccattac tgccagaggg tcgggtacat
     2521 caagaaagaa tgaatcggcc acgttctctt cgtcacacct ttctatatcc tccgggaaaa
     2581 aagtcaccct ctctatcttt ccgagagcgg ccacattttc gcgcgctctc atgacgaaat
     2641 cacttctttt ttcgtaggtt atcacccgtc cagaacttcc caccagcctg gaaaagacag
     2701 aagtcattac accggttcca gttcccgctt cataaaccac atccccatct ttgacgtcga
     2761 gattgagcgt aatgaaagcg atatctttcg gatagactat ctgtgtggaa cgcttaaggg
     2821 ataggattcc atccatgaag gacggcctca acacataagc tcccttgaat cccagcgata
     2881 cgaaagaacc gtactccata ccggctagag aggacatgat cagattcccg taatgggtcg
     2941 tcaggacttc tccttcccta acttctgcga aaagcctcgt ctcttcatct tcgagtagaa
     3001 tcacaactct atcgccggct cttatcattc agattcctct caatttgata atgttttcga
     3061 tcagattatc cagctcttcg gttttaccgg cttcgttggc ctcaataacg cattttctgg
     3121 cgtaaccttt gattatcatc tccgaaaccc tagagagagc cgaattgact gcggaaacct
     3181 gtgtgagaat gtcgccgcaa actctgtcat tctcaaccat cttttgcaga cccctgatct
     3241 gtccctcgat cctcttcaac ctgttaagga cagcctttct tgtactctca tccacaccct
     3301 gcatttcctc tctcctttat ttcttcagtt tcttcagtaa ctgaacacac taccgccgat
     3361 gaattccttt attatggaag ttcttggcac atcctcgaga ttggtgatgg gcaaaaagta
     3421 atctatgaac ctcaaaagcc ttttcgcttc tacgaactcg ggtaccgaat cgtctatctg
     3481 gacaaggaga ggttctgcga aactcttaag gatacccagc tcgtaagcca gtgcggagtt
     3541 gaacatagca tcggcctctt cctggaaggg aaaaatgtac ctgtcctcgc cccttctgac
     3601 actcgaccac atccttatcg tatccagagc cgaatgtccc ctgaacctgt tgtctctgac
     3661 tattctgcgc aataatctga catcggtggt tggaattctg ttcatgttat ctatgttcag
     3721 ctgtgttagc gcgctcacgt atatcttgaa ttttctttct ttcgggatcg aagccgtcag
     3781 tagctcgttc agaccgtgaa taccttccac gattatcggc tgatcgtggc ttatcgaaac
     3841 ggccttttcc tgaaaaccgc tcttcccgag aaggaaatcg aactttggaa gcaatacctc
     3901 tctaccctcg agtagttcac ccatctgtct gttgaagagc tcgatattta gcgcgtatat
     3961 cgattcgaag tcgtaatttc ccttttcatc cagcggggtg tccttcctat cgaagaagta
     4021 atcgtccagt gagatctgga tcggattcat tcccagcact ctcaattgca acatgagccg
     4081 ctttgaaaag gtggtcttcc ccgagctcga cggcccggca acaaggataa ccctcacact
     4141 ctcgcggctc tctatctgat cggcaattcc tgctatcttc ttctcatgga gagcttcggc
     4201 aacatttatc atctctctcg tttcaccgta tcccttgctt attatttcat tcaattcgcc
     4261 tatcgtctta acccctagta tgcttcccca gcgttcgtgt tctagaaaga tactggaaat
     4321 tttcggttga tccacgaaat ccggtaaggc gtgaggttcg ctcgccgaag gcaggttcaa
     4381 cacgaaatag ttgccgtaag gtatgagcga gaaataaccc acagatcccg tggagaaagg
     4441 catataaccg taaaaatagt tcaggtaatc tttgcatcgg tatatattca ctgtggattt
     4501 ttttcgatac ttgaagagaa gagccttatc tatctggccg tcttccctga aaatccttat
     4561 agcctcgaac tttcctatgg tatcctttat gaacgggacg ttttcttggg ctatctctct
     4621 catcttttcc tcgattttct ccaggagttc ccggttcgca acgacaccct ctatttcaca
     4681 gatcaagccg gaacccctcg aatgatgaac atcaagcgag acctttccga gtagctccct
     4741 gacagccatg aagagtataa atatggctcc cctggtatag atccttatcc catcgagtga
     4801 actgagatcg acgaattcga tctccttctc ctcgcccaca agtctacaga gttcaaaaag
     4861 agagtttccg gatttcacag ccattattgg agacccgtac ctgccggaaa accggcaagc
     4921 cagatcctga aaggtcgttc cttcggacac cggaatcgat tcctcgattc ctttgattcg
     4981 tattgttttc tttgtcatac tcttatcacc gcttcctgaa tcgttgacag catcgaaagc
     5041 cgatgctaaa atctactagc gatggggcgt cgccaagctg gtaaggcatc agattttggc
     5101 tctgacatcc gtaggttcga atcctgccgc cccagccaaa aaaagggtgg ccgaagccac
     5161 cctttttgta tgtatcagtt ttccgttccg gtggcttcta cagtagtgcc ggttgccagt
     5221 tcagagatct ggaactggac ggtctctatc gtcgatttca ccgccgcatc ctctggatac
     5281 agttcctgaa gcatctccag ataacccatc tctcctgtga gatacatccg catcatctgt
     5341 tcgtcttcct gtatattcgc gagtttctca tatatatcca ccagcagata aagtgtgctg
     5401 actttaagat cggttgcaac atccgtaacg tttagtattt tcatgttcag gtcgctctcc
     5461 acgctctgaa cgggagaatc gatcaaaaga gaaaccgcct gggcaccgaa gaactgaccg
     5521 tagtactgga gatagctatc cagaagcgcc ggattctcca ggagcggtct gatatttctg
     5581 ttgtaaatac tctcgttata gagatagacg atcttcgggt catttccatc tatcctgtac
     5641 atgtagttta tcacctgcga ggaattgggc atcgccgagt acaaaaactt tacactgttt
     5701 tcgaaataag tgttggtcct gtcatacact tcgttctttt ccgagagcag tttctccaca
     5761 tcagtcagtt ccgataagcc gtacttgtcg aggtaatcgt acgctctctg cgaattggaa
     5821 aggtataccc tcattatcga atccgtggac ggggtggttt caagctgagc cgcgatctct
     5881 tctctggata gggaagttat actcgccgga acagtggtca atataccttt aaggtcctcc
     5941 agatcgagga cttcattcct gagttcagaa gctctgtcgt ccgcgagttc ggtgtagata
     6001 accatcggta ggtatgaatc acctatcaat acgttgcctt cgccgtcgaa atactcgttc
     6061 tgaagctcag tcatgagctt ttgcgaactc tcggaatcgt ttctgctgtt ggcgaatttc
     6121 tcgtacatct ttaggtccgg atcgttgatg gcgtaggaga gtttattctc ctccttataa
     6181 ccggaaagcc acgacgcgaa gcgttcatta ctgtactggg agtactcagt atcgtatccc
     6241 gctactttta cgaactcttc gtacgtatcg aaggtcgtct tggtcgccac gtctatcagg
     6301 tgatagccga actgtgatat aacaggtcct gtgatgactc caggagttgc cgcaaaagcg
     6361 gcatcttcga attccttcac catctgtccg tgcccaaact cgcccagatt accgccgtca
     6421 atcgcggaac cggtatctat ggaaaattcg ctggcggcct gagagaaaga cagagcccct
     6481 tcttcgatct tgaccttaag ctcgagagcg ctggcttctt cggttacgag aatatggctg
     6541 gcggttacct tttcgtattc tgacttgagg tcgaccatgt tatctgccac atactgcttg
     6601 aaatcttctt cgatgttagg aagcgcctta gcctgaacat tcaacgagag aagttgtgtc
     6661 gatacataat cccgcactat ttccgagaag gcctctaccg atccgtactg atagattatg
     6721 gcggccttgg tgttctcatc gcccacgtac tgattgacta tgcgatcgct ctcggatttt
     6781 atctgttcgg cggtgggaac tgcgttgttc tcaacggcgt atagcaaaag ggttttctga
     6841 tctacgagag atctgaaaat ttcatatctt attcccatct cgctgggatt gttttcaatc
     6901 tcaaagtatg gatccagatt gtaaccctga ctcctgatat tgctcagggt atctctagct
     6961 gaaatctcgt attccgaata attgatccag tacctggaat cggtcaccgg agttccatcc
     7021 ctggtgagtc caccgaccat ttcttcgaac ttagctgtct gatcgacgtt ctgcgtatta
     7081 ttctgagaac cgcttccgag gtactgggca accgtccacc agacgattcc gatcccgaac
     7141 agtatggcca cggcccatac taccgggcgt tgaattgatt tcataaaatt ggtgtttctt
     7201 cttggcttct ttgccgacat ctacttttca actccctcca cgtaccgata ctctacaacc
     7261 aacaagggga ggtaataagc ctcccccgcc ttcaggaatt ataccacagt cataattgac
     7321 ttgttaatgc acacttgaag aaaacgatta cggtgtaccg actactaacc gacctgtctc
     7381 gaatagactc tgaagtctat ggaagagaag atgccgtcta tccattccag tgttttgagt
     7441 ctctctacat ctatggtacc tgtttctatc tctccataaa gagtgagaag tctgttgatg
     7501 tgcgtcttcg tacggttaac ggcgtattca acagttgtgt tggttgttat tatgaaagcc
     7561 caatcgctcg attgggccag aagtaactct ctgaccatct ggttcaaagc ccttattttg
     7621 agtctgtctt cggttttttc gtattttctt gcgagctcga tcattctcct gacaccttca
     7681 tcgagatgtc tgtaaatcca gtcgtttctt ccgttcagcc acacttcgtt ataaccgttt
     7741 gctccccatg tggagatgtt tggtgttaca cactgaactt cgcccatcga atcgattacc
     7801 tccggcggtg tcgcgcagac cacggtatcg tctccgttca attctctgaa gagttcctct
     7861 atgaagatcg gtccctcata ccaccagtgg ccgaaaagct ccgcgtcaaa aggagcgaca
     7921 actaccggac tcacaccatt gaactgttca gagagcctct tgatctgctc gcgttttctg
     7981 gcggcgaaat ctttagcatg ctccctggtg gcttttttcg cttcttcgaa atcgtagtag
     8041 ctcttctcgt tcagagagac attcttgcca gttattctgt agtatttgat acctgtgttg
     8101 caccgctcgc cgctgggatc tatgtaaggc cttatgtaat cctcttctct atcgaacccg
     8161 acatccctgt agaactccct gtatctggaa tctccgggat acccaacctc cgcactccat
     8221 acctgctggc ttgactccgg gtctcttgca aagacaaaca ctccctctgg agtcatgacg
     8281 ggtctataaa caccgtatct gggagcgggg tctccgtacc agagcccatg ggagtccacg
     8341 aagaagaagg agaggcccca ctctctcaag taagaatcta tgcccggaac gtaaccgcac
     8401 tcggcgagcc agattcctct tggagccttc cccatgtgtc ttttgtaggt atccactcca
     8461 actccgatct gggcgttcac ggcttccgga tactgtgtca tgagagggag aaaaccatgt
     8521 gtggcgttgc aggtaacgat ttccaggttg ccgctatcct gaagctgagc gaattccccc
     8581 gagaggtcgc cgttacagcg tatatagacc gacttggtct cttcgagttc cttgagatag
     8641 tgcttcgcca acctgttact ggcggggttt tctgagattg ttcgctcgat ctccttctcg
     8701 gccagttcta tggataaatc gagatacttg aggtatttct tctgcaaatc cgcgttggcg
     8761 aacatttcca tcagcggagg ggttatactc atactcactc tgaagtgtac gccgtcgttt
     8821 ttcaagcgat tgaaagcctg aacgagcggg atataagtct ccgtcatcgc ctcgaagagc
     8881 cagcgttctt ccatgaagct cggaaagtcc ggatgattta tgaagggaag atgggcatgg
     8941 agcatcagca cgaatttacc ttttctcatc ttatcatctc ccgcttctag acttggaaat
     9001 acttttgatg aattcttcgg aagacgggtt gtccgacggg cctgacgacc tgatcgatag
     9061 agaggtctcc gccgcttcaa ccgaaactct aacttccttt ttagagcctt tcgatagatc
     9121 cacccaggtt tcttgatcgg tgaattgtgg agtgttcctt ggagtccggg cgatttttga
     9181 tttcatgaaa cctacgaaaa gctctccggc gtaataccct atctccgcga tatagtcggc
     9241 gtcgggaaaa tctacctgaa agtaccagtt accttcctcc gataggatct tcgcttcttt
     9301 atatctgttg gcgttttttc catcgtagat tatgttggtg atgtcgctaa ctctcacaaa
     9361 gatatcgttt ccgagttcat ctaagcgttc tctggtctct ttggagaagt cccatttaat
     9421 atatacccag taaggattta ccggatgcaa tgttatagaa tcggtgaaat aagtcctggg
     9481 aagatcaacg tttacaggct ttgaaggtct tttactttcc gagggagtcg ccacaacggt
     9541 ctgactgctc accggtacat ctttatccga gagtttcgcc agagccctgg cgatatcctt
     9601 tttgcccatg gttctcttaa gatgaacacc gagcatcttc gcgtatcttc gcatttcctg
     9661 aatcgacgga tcggttttta aaaaagaacc gatatcttct ttcttcataa cttgaaacct
     9721 cccgggcatg attttcactg tatataatat aacacaccat aggaggaaat atcaagacaa
     9781 taagagcaat tattggcgat tatctaattc tttctttcct ggataacaca aatcgcggtg
     9841 ttatctatat atctctcagt ttatcgataa tataatcgac catgtaatct ccgtcaacct
     9901 gatattgaga accatccatc attattttca ccgtcactag gtccttttcg agttcctcgt
     9961 cgcctatgat gaccgagatc ttagccccta tcctggcggc atgtttcatc tggtttctca
    10021 tatttctctc catcacatcg acgtaagtgg aaataccggc cttcctgagc cttctggcgt
    10081 actcgaatcc tctcaaagat tcctctctgc cggtcgcaag aatggcgacc tcggaatttc
    10141 tgtcggtcgg gatgttaact ttttcggcct tgaggagcat gactatcctc tccatgccga
    10201 tcgcaaaacc cacggctgga cagctcttgc ctccaatcac ctcgacgagc ccgtcgtacc
    10261 tcccgccgcc gactatctga tcctgactgc caagtgacgg agaccttatt tcgaaggctg
    10321 ttctactgta gtagtcaagt cctctcacca gatggggatc gatctcgtaa tcgattctgg
    10381 ccttttccag ataccacttc aaatcttcga agtgtttcct gcactcctcg tcaagatact
    10441 ccagtatgtt cggggcgttc tcggactgct ccctgtcgat cttgcagtcg agcaacctca
    10501 aaacgttgct gtcgaacctc cgtctgcagt cttcgcacat gccgtctacc ttatcgcgaa
    10561 agtactccct cagggcctct ctgtaccggg ctctgcagga aggacagcct atggagttta
    10621 tgaaaagcct ggtcttgttt atcttgactt cttttaaaaa ctcgcaagcc agttcgatta
    10681 tctcaaaatc cgccatggca ctggccgatc caagtatctc cgctccgaac tggtgaaatt
    10741 gcctgagcct gcctgcctgg ggtttttcgt aacggaacat tggtcccatg tagaagagct
    10801 tcactggaga acccagattt atgaaagaat
//
LOCUS       DAOOLY010000057        10820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6883_length_10820_cov_5.150023, whole genome shotgun sequence.
ACCESSION   DAOOLY010000057 DAOOLY010000000
VERSION     DAOOLY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6883_length_10820_cov_5.150023"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PLD15_04390"
     CDS             <1..1177
                     /locus_tag="PLD15_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HPB63173.1"
                     /translation="PQNRLIVRPNNDTIYSMIWLELSTEPIVISIPEITDRYYSFQLV
                     DMYTHNLAYVGTRATGSDAKTVMIVSPCWEGETPPGIDEVFYSEGNYVYCIVRTGVDS
                     ELPGDLQRVLEIQQQYLLQPLSNYYGTPALEELHAVKLPPFDQTVADSAGFIGYFNFL
                     LGQLKIHPSEEALIQSFSKIGIGPDYPFDPDNLTEPVLEAIESAIAEARDEISAMEKE
                     IGIVKDGWSLSERIFGSRERMHGLYLIRATAAHIGLYGNDLEETFYPNCLYDSDREPL
                     DASRYNYVINFAKEQLPPVDPKGFWSITMYDEEQFLVDNPLNRYSIGDRSELTYGEDG
                     SLVLYLQHESPGESKESNWLPAPDGKFKITMRIYIPLPEGLDPLYCPPAVRKAGVVE"
     gene            1494..2606
                     /locus_tag="PLD15_04395"
     CDS             1494..2606
                     /locus_tag="PLD15_04395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB63174.1"
                     /translation="MSLSGYFLYDSIRDLIVYELGNNAVNIAFTAAKFIEQDIDPFIA
                     LAEVEDYDLQEYDTDYYNRMQSLFRELKKNTGATFIFAEKKRSDSEIVYLIDGEDPQD
                     ELFSPIGTVDSMGEFELRVFKECITGATDIVNWEFWGMFVTGYAPIVDRSKDEVVGVV
                     GVDFSFEYIENLLNRIRQLIIFTFLLIALLGTYILNRLVIDRSTALSIDYLTGLHSKR
                     YHDARLNSLIRKSRFSGKPLSLMIIDVDSFKEINDEFGHNVGDETLKVAARKIKLYTR
                     STDICSRVGGDEFAIILPETDLERARLVGERIVERFKRDLSSTKKDGGPKITLSIGVA
                     QWREGMSARDLNQLADVAMYRAKSKGRNGVFAHQHR"
     gene            2648..4126
                     /locus_tag="PLD15_04400"
     CDS             2648..4126
                     /locus_tag="PLD15_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB63175.1"
                     /translation="MSKVIVIGAGIGGLAAGCYARFNGFQTTVLESGERAGGLCTSWN
                     RDGFTFDGCIRHLAGLKPETALYKMWSDLGAAPAEAFFPEELVKVIDESGREFTVYYD
                     TNRLEEEMKRLAPEDSDTIEQYIKGIKLLSRIELMEVGFWKFRDWLKNIPTLMKSGKY
                     SRESMNKFAERFSSPILKKFFPVIQYGWKNIPLTLHLAVMAGSSRKMYGRYAGGSYEF
                     SRAVEKRFLSLGGELRYGTRVEKVLTDNGVTTGVQLESGEKITADYVISDIYGYDTFM
                     KLLDDRYLTDRFRSMYSRPIDRMMMAIHVSLGVGMDLSQVPNSIAFFPDRPPEIAGEK
                     HEMLSILNYGYDPSMAPKGKTALKVMYDTSYSYWEKLYKDRQAYEDEKRKISGNTIQI
                     LDRVFPGLSDAVEVIDVATPMTTKRYTANGQGFGRKEDFRLKDTLRGFLNRPLTLESL
                     KNFFVIGHSVGGTNLYGCAAMGMNTVKLLCKREKRRFLASVK"
     gene            complement(4162..6771)
                     /locus_tag="PLD15_04405"
     CDS             complement(4162..6771)
                     /locus_tag="PLD15_04405"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HPB63176.1"
                     /translation="MIPHHDSLTEILKQLGVNPEVGLSAEEAALRLESHGENRLREEK
                     KKGNFRRFLEQFKDIMIIILMAAAVLSFAVSFYEGEGFIEPVLIILIVVLNAIIGVVQ
                     ESKAEKALEALKRLSVPHARVLRDGEESMIESTGLVPGDIVLVEAGNFVPADGRLISC
                     ASLKVEESALTGESIPSEKSAETTVPGDAPLGDRHNMIFSGCSISYGRAKAVVTATGM
                     DTEMGKIAGLLAGEEESSTPLQRKLATLGKYLGIAALLVCAVIFVIGLIDGIPLMEIF
                     MISVSLAVSAIPEGLPAIVTIVLAIGVQRMVKRNAIIRRLPAVETLGSASVICSDKTG
                     TLTQNRMTLVKAFDGSTSILEDVGSDNSPSIRKLLLYAALCCDGKVVFEDGMEKHIGD
                     PTETAIILAARENGMMKEELDARYPRLAEIPFDSERKMMTTLNLIDGKKIVIVKGAFD
                     VLSGRCVEGKTREAREYVDRMGRDALRVLAVAFKEIDEVPAEPEPEILESGLTFMGLV
                     GMIDPARPEAKEAVALCRKAGIKPVMITGDHSLTASAIARELGILDDGDETVTGSELS
                     AMTESELKERVGKISVYARVSPSDKIRIVRAWQERGEIVAMTGDGVNDAPALKAADIG
                     CAMGITGTDVAKGAADMTLTDDNFATIVEAVKEGRGIYDNIRKVVGFLLGTNIGEILT
                     VFFAMLLWREAPLLSMQLLWINLVTDSLPAIALGMEPVENDVMNRRPRRRRENIFANG
                     LGVQVLLQGMLFSALTLMGFIIGRMTGDIATGRTMAFLVLSLTQVVHSFNMRSSRSLF
                     KIGPFTNRTLTLAAVVSFAMIALVSFVPPLATIFGLTTLSYGLYLVAALLAFVPLPVL
                     EATKMIGLVRHRK"
     gene            complement(6894..7610)
                     /locus_tag="PLD15_04410"
     CDS             complement(6894..7610)
                     /locus_tag="PLD15_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB63177.1"
                     /translation="MKNDDGQETRERIINASIQLFSQKGYDATTTGEIASMAGVTKGL
                     IYYYFESKEEILNHLVDSLMKDATTIAMDFVHTGIVQMIRDGKLDIETDRLRFIDGGA
                     IRDFSRKVIDYYERALDFVLEKRAVIRILMLESLKDSKHHNDLFRLMELTGDGEDNPV
                     FKTIYEADRDFTYSGDMSVFKFFFSIVPLINFAAHYEEYKVISSMSDRELKDSFLRSF
                     KIIFASLVSGSEILLRNDKT"
     gene            complement(7869..8201)
                     /locus_tag="PLD15_04415"
     CDS             complement(7869..8201)
                     /locus_tag="PLD15_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63178.1"
                     /translation="MKRIITLALLLLVVFGLVFANNGNGPGDGISDGPDDGAGYGSPG
                     NGQGDGPGGGKLDSGERTVALPAVRPIFEKSEPLFFTLLKEFIIMMRNAFKSFNFSGV
                     AMLQIGGR"
     gene            8546..9619
                     /locus_tag="PLD15_04420"
     CDS             8546..9619
                     /locus_tag="PLD15_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB63179.1"
                     /translation="MRGAIVITSKYGIIERYLLEELKSGKYSAGDKLPTEKELMTKFN
                     ASRETVRKALDRLTLKAVIVRRPGLGTFVSYGNDNHLVGILVQQITSYIFPYVVLGAE
                     DYLFRNEYKMLLGNASEDPVKERQILGEWMESGVKGLIIDPVYSATKRSNKDFVKSLT
                     KSGIKITLVHTDWNIDQVSCVILDDASGGEKAAEIFYDYGHRRVAVIYKSTHLPGVVR
                     ARSFIERCRQLGVDRIYDKAFNVSEFTGAPMQIAHELMSLPLQIRPTAIFCYNDATAL
                     QLQLVAKRLALRIPDDISVIGFDDGPIGDFRDVLTTFAHPKEEVGRRAVEILLDMLNG
                     GKPQKVVFKPELIERSSITQAKS"
     gene            10239..>10820
                     /locus_tag="PLD15_04425"
     CDS             10239..>10820
                     /locus_tag="PLD15_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB63180.1"
                     /translation="MKKFMCLVLALIVFVGFSFGVTVTMMTPLTGADGAYMDEIVAKF
                     NATHPGIEVVHVVVESSLDMKNKLSMGIASKTAPEIMFIRKFDMPLYLQHFKGFTPDE
                     WLNNYGIDVNDIFPGVLEGLVIDGKVVGIPLDIWIFYMAYNKANFAKVGLDPENPPKT
                     RDEFIAAMEALIPITPAGLTPYYENPAWTWIWFH"
BASE COUNT         2748 a         2651 c         2688 g         2733 t
ORIGIN      
        1 cccccagaac agactaatag tccgtccgaa caacgatacg atatactcaa tgatatggct
       61 ggaactctct accgagccca tagttatctc tattccggag atcaccgacc gctactactc
      121 cttccagctg gttgatatgt acactcacaa cctggcgtac gtggggacga gggccaccgg
      181 ttccgacgcg aagactgtga tgatcgtaag cccttgctgg gaaggagaaa cccccccggg
      241 tatagatgag gtattctaca gcgagggaaa ctacgtttat tgcatagtga gaacgggtgt
      301 tgacagcgaa ctgcccgggg atctacagag ggttctcgaa atacagcagc agtatttgtt
      361 gcagcctctc agcaattact acgggactcc ggcccttgag gagcttcatg cagtaaaatt
      421 gccccccttc gatcagactg tggccgattc tgccggtttt atcggttatt tcaacttctt
      481 gctcggacag ctcaagattc acccctccga ggaggccttg atccagagct tctcgaaaat
      541 cggaatcggt ccggactatc ccttcgaccc cgataatctc accgaaccgg tgctggaggc
      601 aatagaatcg gcaattgctg aagctcgcga cgaaatcagt gcgatggaaa aagagatagg
      661 gatcgtaaag gacggctggt ccctgtcgga gaggatcttc ggaagcagag agaggatgca
      721 tggcctgtat ctgataaggg caacggctgc ccacatcggg ctttacggaa acgatcttga
      781 agagactttc tatccgaact gcttgtatga cagcgataga gaaccactcg acgcttccag
      841 atacaactat gtgataaact tcgccaaaga gcagttgccc ccggtggatc cgaaaggctt
      901 ctggtcgatc accatgtacg acgaagagca attcctggtc gataaccctc ttaacagata
      961 ctcgatcggc gatcgatctg aactgacata tggcgaggac ggctcgctgg tgctgtactt
     1021 acagcacgaa tctcccggcg agtcgaaaga atccaactgg cttccggcac ctgatgggaa
     1081 atttaaaata acgatgcgaa tctacatccc gctgcctgaa gggctcgatc cgctctactg
     1141 tccgcccgcg gtgcggaagg cgggggtggt tgaataattc gatgttgcta tattcgattt
     1201 gttagaacaa taaatatgat tgatacattt gattcattag tttcgggttg tcttaagttt
     1261 aataaatata ttcatggaca tttgagccca tcggtagtat atttattgcg atgtgatcaa
     1321 cttgtatgtt ttaagtgaca aactcaaaac ttgcaggaga tgatgaagcg atgggctctt
     1381 caacaggaac ccctgatgag aaagaaaaaa cgaaggtgga cctgtatgca agaagcttta
     1441 agaacagact gcttctaacg tggctcttta tctttgttgt gatagcggtt tttttgtcct
     1501 tatccgggta ctttctttat gacagcatcc gcgatctcat tgtttacgag cttggaaaca
     1561 atgccgtgaa catagccttt accgcagcca aattcataga gcaggatatc gatcctttca
     1621 tcgcgctggc ggaagtcgag gattatgatc tgcaggaata cgataccgat tattacaaca
     1681 ggatgcaatc tctattcaga gaattgaaga aaaacactgg cgcgaccttc attttcgccg
     1741 aaaagaagcg gtccgactct gagattgttt atttgataga cggtgaagat ccgcaggacg
     1801 aactcttctc tcccatagga acggtagata gcatgggcga gtttgagctt agggtcttta
     1861 aagagtgcat aacgggggcc acggatatcg taaactggga attctgggga atgttcgtga
     1921 ccggttacgc tcctatagtg gatcgatcga aggacgaagt ggtcggggtc gtgggggtag
     1981 atttctcctt cgagtatata gagaatttgc tcaacagaat cagacagctg ataatcttca
     2041 catttcttct gatcgcgctg ctgggaactt acatattgaa caggctggtc atagatagat
     2101 caaccgccct gagtatcgat tacctcacag gtctccacag caaaaggtat cacgatgcca
     2161 gattgaactc actgatcaga aagagtcgtt tttccgggaa acctctctcg ttgatgataa
     2221 tagacgtcga ttcattcaaa gagatcaacg atgagttcgg ccataatgtg ggcgacgaaa
     2281 ctctcaaggt ggcggccaga aagatcaagc tctacacgag aagcaccgat atctgttctc
     2341 gcgtgggggg agatgagttt gctataatac ttccagagac cgatcttgaa cgggccagac
     2401 tggtgggcga gaggatagtg gaaagattta aaagagatct atcgtcaacg aaaaaagacg
     2461 gtggtccgaa aataactttg agtatcggtg tcgcacagtg gcgggagggc atgagcgcca
     2521 gagatcttaa tcagctcgcc gacgtcgcca tgtacagggc caaaagcaag gggcgcaacg
     2581 gagtttttgc acaccagcat cgctgatgcg accgatctta agaagcgcga gataatggag
     2641 gattgaaatg tcgaaggtaa tcgttatcgg cgcaggaata ggtggcctcg ccgccggatg
     2701 ctatgccaga tttaacggtt ttcagacgac cgtacttgaa agcggcgaaa gggcgggcgg
     2761 gctctgtact tcctggaatc gcgacggctt cactttcgac ggctgcatcc gccatcttgc
     2821 aggtctgaag ccagaaacgg ctctttacaa aatgtggagc gatctcgggg cagcgcctgc
     2881 tgaggccttc tttcctgaag aactggtgaa ggtgatcgat gaatcgggaa gagagttcac
     2941 agtctattac gataccaata ggctggaaga agagatgaaa cgccttgccc ctgaggactc
     3001 ggatactatc gagcagtaca taaagggcat aaagcttctt tccaggatcg aactcatgga
     3061 agttggtttc tggaaattcc gcgactggct gaagaatatc cccacgctca tgaaaagcgg
     3121 caagtactcg agggagagca tgaataagtt cgccgaaagg ttctccagcc caattctgaa
     3181 aaagtttttc ccggttattc aatacggctg gaagaacatc ccgctcactc tccacctggc
     3241 ggtcatggcc ggttccagcc ggaaaatgta cgggcgttac gctggagggt cttacgagtt
     3301 ttccagggcc gtcgagaagc gtttcctctc gctggggggt gagctcaggt atggaacccg
     3361 ggtcgagaag gtgctcaccg ataacggtgt gacaacggga gttcaactcg agagcggaga
     3421 gaaaatcact gccgattacg ttatttctga catctacggc tacgacactt tcatgaagct
     3481 cctggacgat agatatctga ccgaccggtt ccgttcgatg tactcccggc cgatagatag
     3541 aatgatgatg gcgatccatg tgtcgctggg agtgggcatg gacctctctc aggtgccgaa
     3601 ctcgatagcg ttctttccag acagaccccc tgaaatagcc ggggagaaac atgaaatgct
     3661 cagcatcctt aattacggct acgatccctc gatggctccg aaagggaaga ctgcccttaa
     3721 ggtcatgtac gacaccagtt actcttactg ggaaaagctt tacaaagaca gacaggctta
     3781 cgaagatgaa aagagaaaga tatccgggaa caccatacag attcttgaca gagtcttccc
     3841 gggtcttagc gatgcggtgg aggtcatcga cgtggccacg ccgatgacta cgaaaagata
     3901 caccgccaac ggtcagggct ttggccggaa agaagacttc cgcctcaaag atacgctcag
     3961 gggtttttta aacaggcctt tgacactcga gagcctgaag aacttctttg tgatcggcca
     4021 ctccgtcggc ggtacgaatc tctacgggtg tgccgcaatg gggatgaaca cagtaaaact
     4081 cttgtgtaaa agagaaaaga ggcgatttct cgcctctgtt aagtgaaagc cgtattgaag
     4141 gattcttcgg tcgcgaaacg ctcacttcct gtgccttacc agaccgatca ttttggtcgc
     4201 ttcaagtaca ggcagcggta cgaaagccag cagggcggcg accagataca gaccatagct
     4261 aagcgtcgtc agaccgaaga tggtcgccag aggcggcaca aacgaaacaa gagctatcat
     4321 cgcgaacgac acaaccgcgg ccagagtgag ggttctgttg gtgaacgggc cgattttgaa
     4381 cagggaacga ctggaacgca tattgaatga atgtaccacc tgagtcagtg aaagcacaag
     4441 gaaagccatg gtacgtccgg tcgctatatc gcctgtcatg cgtcctataa tgaatcccat
     4501 gagagtgagg gcggaaaaca gcattccctg tagcaaaacc tgtacgccga gcccgttcgc
     4561 aaagatgttt tcccttcttc gcctcggtct tcgattcatc acgtcgttct ccacaggctc
     4621 catacccagc gctatagccg gaaggctgtc ggttaccaga ttgatccaga gaagttgcat
     4681 cgatagcaga ggcgcttctc tccacagcaa catggcgaag aaaacggtga gtatctcccc
     4741 gatattcgtt ccaagaagga agccgaccac ttttcttata ttgtcgtaaa tgccccgccc
     4801 ctctttcacc gcctcgacta ttgtcgcgaa gttgtcgtcc gtcagggtca tatcggccgc
     4861 ccctttggcc acgtcggtac cggtgatacc catggcgcag ccgatatcgg ctgccttaag
     4921 agccggagcg tcgttcacgc cgtcgccggt cattgcgacg atctctcccc gctcttgcca
     4981 ggcccggact atacgaattt tgtcggaggg tgagactctg gcatacacag agatctttcc
     5041 gactctctct ttgagttcgc tttcggtcat cgccgacaac tcgcttccgg ttactgtttc
     5101 gtcgccatcg tccagtattc ccagttccct ggctatggca gatgccgtga gcgagtggtc
     5161 accggttatc ataaccggtt ttataccggc ctttctgcac agcgcgacgg cttctttcgc
     5221 ttcgggtcgg gccgggtcga tcataccgac cagtcccatg aaggtcaaac cgctctccag
     5281 gatttcgggt tccggttcgg ccggtacttc gtcgatctcc ttgaaagcca ccgcaagaac
     5341 tctcagggca tcgcggccca tccgatcgac atattcgcgc gcctcacgtg tcttaccttc
     5401 gacacatctg ccggagagaa catcgaaggc acccttgact atcacgatct tttttccgtc
     5461 tatgagattc agcgtggtca tcattttccg ctctgaatcg aacgggatct ctgccagcct
     5521 cggatagcgg gcgtcaagtt cttccttcat cattccgttc tcgcgtgctg caagtattat
     5581 ggccgtttcg gtgggatcgc ctatatgttt ctccatcccg tcctcgaaca caactttgcc
     5641 gtcgcagcag agcgcggcgt aaagcaaaag cttccttata gaaggagagt tgtcgcttcc
     5701 aacatcttcc agtatcgacg tcgatccatc aaaggctttg acgagagtca ttcgattctg
     5761 tgtgagcgta ccggtcttgt cggagcagat caccgatgcg cttccaagag tctcgacggc
     5821 cggcaacctg cggatgatgg cgttgcgctt caccattctt tgaacaccta tggcaagcac
     5881 gatcgtgacg atggccggga gtccctcggg aatagctgaa actgcgagcg atacggagat
     5941 catgaagatc tccatcaaag gaattccgtc gatcagccct atgacgaata tcaccgcgca
     6001 gaccagaagc gcggctattc ccaggtactt gcctagtgtt gccagctttc gctgaagggg
     6061 agtagaactc tcttcttcac cggccagaag accggcgatc tttcccatct ccgtatccat
     6121 gcctgtggcc gtcaccacgg ccttcgcacg accgtaagag atgctacaac cggagaagat
     6181 catgttatga cggtctccaa gtggagcatc gccgggtaca gtcgtctctg cgcttttttc
     6241 cgacgggatg gattcgccgg tgagtgccga ttcctctact ttcaagctcg cgcaggatat
     6301 gagccgtcca tcggccggta cgaaattgcc ggcctctacg aggactatgt ctcccgggac
     6361 taggccggtg gactcgatca tgctttcttc accatccctc agaactctgg cgtgcggtac
     6421 agagagtctt tttagggcct ccagagcctt ttcggctttg ctttcctgaa ccaccccgat
     6481 tatcgcgttc aacacaacga tgagaatgat cagcacaggt tctatgaagc cttctccttc
     6541 gtagaatgaa acggcgaaag ataggacggc cgccgccatc agtatgatta tcatgatgtc
     6601 tttgaattgc tcgagaaaac gccggaaatt tcccttcttt ttttcttccc tcaatctgtt
     6661 ttcaccgtga ctttcaagac gaagtgccgc ttcttcggcg gagagtccca cttccggatt
     6721 gactccaagc tgcttcaata tctctgtcag actatcgtgg tgcggtatca tattctcttc
     6781 tccttccttc cgtaatgaaa taactctgta tcgatcgtta ctgactgaac gttcagtcaa
     6841 taagtgaaaa aaacttacat gtgtcggcgg cacaggctat gatctaaata cctctaggtt
     6901 ttgtcgttgc gcaagagtat ctcgcttccc gatacgagcg aggcgaagat tatcttgaaa
     6961 gagcgtagaa aagagtcttt caattccctg tcgctcatcg agctaatgac tttgtactcc
     7021 tcgtaatgag cggcgaaatt gatcagcggt actatggaga agaaaaactt gaagaccgac
     7081 atatctcccg aatatgtgaa atccctgtcg gcctcgtata tagttttgaa tacggggttg
     7141 tcttcaccgt cgcccgtcag ttccataagc ctgaacaggt cgttgtgatg tttgctgtct
     7201 ttgagagatt caagcatgag tatgcgtatg acggcccttt tttccagcac aaagtccagc
     7261 gcgcgctcgt agtaatctat aacctttctg gagaaatctc gaatcgctcc cccgtcgatg
     7321 aaacgaagcc tgtcggtttc gatgtctagt ttcccatctc tgatcatctg aactatgcca
     7381 gtgtgaacga aatccatggc gatagtagtc gcatccttca tgagcgaatc aaccaggtgg
     7441 ttgaggattt cttctttgct ctcgaaatag tagtaaatga ggcctttggt cactccggcc
     7501 atcgaggcga tctctccggt cgtggtagca tcgtatcctt tctgtgagaa aagctgtatt
     7561 gaggcgttaa taattctttc tctcgtttcc tggccatcgt cgtttttcaa gcgaagtccc
     7621 cttgctgacc ggtcatttaa ttcaattata tacacttcat gatatctgtc aagtcgctgg
     7681 catcgcttca ctatgacagg cttgatttct ataattaacc gcagtttgtg attggctgtc
     7741 tgctaataaa gaccagggct aaacgagttg tctgcatacg atgaaaaaac ctacaacaag
     7801 aggtgggccg taatgtgctt tcagtcacgc ttattgtagg tatttgccga tagacctcac
     7861 gatgatgttt agcggcctcc gatttgaagc attgcaactc ccgagaagtt gaaactcttg
     7921 aacgcattcc gcatcataat tatgaactct ttgagtagcg tgaaaaacaa cggttcgctc
     7981 ttttcaaaaa tcgggcggac ggcaggcaat gccaccgtcc gctcaccgga atcgagtttc
     8041 ccgccgcccg gaccgtctcc ctgaccgttt cccggagaac cgtaacccgc accatcgtcc
     8101 ggtccatcgg agatgccgtc gcctggaccg tttccgttgt tcgcaaacac caaaccaaaa
     8161 accaccaata aaagaagggc aagtgtgatt attcgcttca tactattgac ctcctgagag
     8221 aatttatgaa ataagtatag aagaacatcg attaaagaga gattaaaaac tatcatcgca
     8281 tctcttcagt ctctcccctt ctcgctcttt ccagggatgg gtccatgctc ctggacgaaa
     8341 cgaaggacgg ctcttcactc tctttcgctc ttgccagact ctagacccca cacctctgac
     8401 cccgctctcc ctataagggt ttagcatttc ataaatcagt aaagcttgat ctgaaaagct
     8461 tttcatagta gttattgtac agattagtaa aacttgactc aatccaattt acggtgtaga
     8521 attcaaatac gtacgtacat ttaagatgcg gggggcgatc gtcatcacat ccaaatatgg
     8581 gatcatcgag cgctatttgc ttgaggaatt gaagtccgga aagtattcgg ctggcgataa
     8641 actcccaacg gaaaaagagt tgatgacgaa gttcaacgcc agtagagaga ctgtcagaaa
     8701 ggcactggac agattgactc tgaaagccgt cattgtccgc aggccaggcc ttgggacctt
     8761 tgtcagctat ggaaacgata atcatctggt tggaatactc gttcagcaga taaccagtta
     8821 tatctttccc tatgtcgtac taggtgcgga ggattatctt tttagaaatg aatacaaaat
     8881 gcttctggga aacgcttccg aagatccggt caaagagaga cagattcttg gcgagtggat
     8941 ggaatccggc gtcaaggggc tgattatcga ccctgtctac agtgcgacca agagatcgaa
     9001 caaggatttt gtgaagtctc tgacgaaatc cgggatcaag ataacccttg ttcacaccga
     9061 ttggaacatt gatcaggtga gttgcgtcat tctagacgat gccagcggag gcgaaaaggc
     9121 tgccgagatc ttttacgatt atggccacag aagggttgca gtcatttaca aatccactca
     9181 tcttccgggt gttgtcaggg ctaggagttt catcgagagg tgccggcagc tgggagtcga
     9241 tagaatctac gataaggcct tcaacgtgtc tgaattcaca ggagcgccca tgcagatcgc
     9301 ccacgaactc atgtctctcc ccttgcagat aagacctacc gcgatttttt gctacaacga
     9361 cgcaacggcc cttcagctcc agctggtcgc aaagcgttta gctttgagaa taccggacga
     9421 catatccgtt atcggttttg acgatggccc catcggtgac ttcagagatg ttttgactac
     9481 cttcgcccac cctaaagaag aggtaggcag gagagccgtc gaaattcttc tcgatatgct
     9541 caacggcggc aaaccccaga aagttgtctt taaacctgaa ctcatagaga gaagttccat
     9601 cactcaggcg aaatcataaa gagcttagaa ctctaaaacg ggaaaacctc ttcgataatt
     9661 gtgccgccca cgcagggcgg tcttttttct tcgacttcgt tgagcggatt tcatcgagaa
     9721 aaacctgatt aatcttattt gtacggacat ttaagcatca gatattctgt gaaatctgaa
     9781 gaaaacccta caatcacgta aaagagagta cgaatatcgt caatattccc cgcaactcgc
     9841 ttctcacagg tctctttcaa acataactgt gtttgttcgg gaggtttttc gtttggtaga
     9901 atctagcgta agtagatcac ttcatagttt cttttttcat tctgttgctt tggcgttttt
     9961 ttggactgtg ttttttaagt aatttaaact tgaaagggtt ttgcttaaca caataaggta
    10021 cgtacatata agtatttccg gagtccatgc caaactaacg atcgaagcca gcaaatttct
    10081 cccggtcata tcctgttgtc acaatgatgg aaaaatgatt acgaacagag agtttttctg
    10141 gttccatggg gggtgatgtg gcaagggaag aacaaatgtc atcgagttat cctttttctg
    10201 gtcaatcggt tattaccttt tctaggaggt gaaactaaat gaaaaagttc atgtgtttgg
    10261 ttcttgcgct gatcgttttt gtaggctttt ctttcggagt aacggttacg atgatgacac
    10321 cgctcaccgg agcggacggg gcttacatgg atgaaatcgt tgcaaagttc aacgccacgc
    10381 accccggaat cgaggtcgtt catgtcgtgg tcgaatcgtc tctggatatg aagaacaagc
    10441 tttcgatggg tattgcatcg aaaaccgcgc cggaaataat gttcatccgc aaattcgata
    10501 tgcctctgta cctgcagcat ttcaaaggct tcactcctga tgaatggctg aacaattacg
    10561 ggatcgacgt gaacgatatc ttccccggtg ttctcgaggg actagtgatc gacggtaaag
    10621 tcgtgggaat cccactggat atatggattt tctatatggc ctacaataag gccaacttcg
    10681 caaaagtggg cctggacccg gagaaccctc caaagacaag agacgagttc atagcggcta
    10741 tggaagccct gattccaatt accccggccg gattgactcc ctactacgag aatcccgcct
    10801 ggacatggat atggttccat
//
LOCUS       DAOOLY010000058        10815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6895_length_10815_cov_5.518773, whole genome shotgun sequence.
ACCESSION   DAOOLY010000058 DAOOLY010000000
VERSION     DAOOLY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_10815_cov_5.518773"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLD15_04430"
     CDS             <1..195
                     /locus_tag="PLD15_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPB63181.1"
                     /translation="YYVYIKREVRDKLVNMTDPKAIAQLLDLLLVARFLERAGDHATN
                     IAEEIYYIAKGANLKEEMRR"
     gene            192..866
                     /locus_tag="PLD15_04435"
     CDS             192..866
                     /locus_tag="PLD15_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB63182.1"
                     /translation="MRVLIVDDDPDILNIIRTACESESMEVDTATSAEELWRALQSDR
                     PDVILLDIMLPDASGLDLIKRIRSTNPNIPIIFLTARKSDLDMILGLELGADDYVTKP
                     FNPRALVARIKAVLRRTEVSGKLEEQLVIGEALVNLKSYTVTRNGVSEELTRREFELL
                     KILAENPGRVFTREELLDKVWGIDFYGDFRTVDVHISKLREKVGEKFIRTVRGVGYKF
                     VPGDAK"
     gene            863..1840
                     /locus_tag="PLD15_04440"
     CDS             863..1840
                     /locus_tag="PLD15_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB63183.1"
                     /translation="MNEPLLNELSEGILKVDEGLAIVEANSSARKLLGEVEGKALPDG
                     LHVQGISKIVDSFISELKFETDTIYVKDDMTLYLRVKVVPPYIVMRNITNEKLFENAK
                     MDFVNSIVHEFSTPLAVINGYVQLLIEKREKLPEEVLETIVRISRSTNRLARLVEELG
                     LLSNLELQNYRVKVETVNLRELVDEAIGDLESKWKRKKLQIEVSVSPDIYAAADSMLL
                     FRIISNLISNAVKYSSIGEKIEITSYETDRKIFLSVKDNGIGIKSDELPRVFERFYRA
                     SNAKTSGSSGLGLGLSLVKHALNLIGGTIDIRSRYMLGTTVTISFPKKI"
     gene            complement(1877..2881)
                     /locus_tag="PLD15_04445"
     CDS             complement(1877..2881)
                     /locus_tag="PLD15_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63184.1"
                     /translation="MKATVFLLIVTVSVSLIALTPLTVTFKDGEPLSYYREYFDLYPG
                     DREVFRCPLLLVGAGNSLMFLDNGQPLTLCMAGLSTEQLDSSFLDRLLRDRDFSISFE
                     TSQNAVGSHRPAYLWLRYETGYVNANLLLLENGKALLESCRESSLLKWLERFGKYEAP
                     PNGPLQKRSTIRERRFTTIVSIYSAGRAKRLESGIYDLEPEKGPKYDHDAMQSEKSYT
                     INEIFAPEKGRVVEESPTATNEQWNTIVSFSGSSTDFTEPFTVPTGEWRINLSHEGSF
                     FSLTLYNAYDGSIEETLLTATSPGAREIYLHRSGTFYFSIISTGNYEIIVQVKTESSS
                     "
     gene            complement(3137..4129)
                     /locus_tag="PLD15_04450"
     CDS             complement(3137..4129)
                     /locus_tag="PLD15_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPB63185.1"
                     /translation="MRYREYASLTDGFSTGKLNRITDVPRLRVGHFTVDRDRPTVLRT
                     GVTVLRIDDVITKPVWAACNVFNGYGKSMGLVQVDELGTMESHVFLTNTLSIGAVQQG
                     AVKIALREHPGTTSFNAVVMECNDSYLSDIASLSVTDMMTIEAYDGVVEDFPMGSVGA
                     GTGMICFGFKGGIGSSSRVIEIGGREYTLGALVLANFGRTADLKMPGPVDANFEEPQK
                     TGGSLIMVLSTDIPLLPHHLKRVARHLSLSIGILGAPGYHGSGDIALAFSTADRSDPQ
                     ALTIGEDGSTMSQIFKASVWATVEAILDSMFSSPDMKGFRGATPSILDSIRQRL"
     gene            complement(4134..6296)
                     /locus_tag="PLD15_04455"
     CDS             complement(4134..6296)
                     /locus_tag="PLD15_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63186.1"
                     /translation="MFKYIWLYFKRRPLRILAFILVVALGVGASMILGSIFLSFGETR
                     NRISAVNESWITIQYLSSNGRDSRLDEVIEKTLSETFGFSDAIKVDIAYLSYNLFGSA
                     RANFPVYGIRQTDIPRLLRESEMQIARGTVFERDSRDIMVSGSFLKADRLEVGQIYEE
                     TLEMSTPGQYSIVASLKGPSIFGLGPAGISSGGGTYGYMIFAREGFLMAVENELKNAL
                     VEYMPSITVNGPASSIDRIREQYAGYYTGVFFVNLVVALIFTVGLTMLNSVSLRERRK
                     EYGVMSAVGYTGGSLKVRLLIESSIQATLGWIAGFLAGVWTISVLEKRFFEPAGLFLR
                     NDPVGSFYTLIIPLAVVLLTQFLITGHLKRDLINLLRSASREKGIFRSRFRDIRTGWL
                     RYPVRTGAYTPLFVNVVVFVALIFFFGSLLSGLSDGIRDGGIFFENRTYVRMADGVEF
                     PSDITSSPEVKSFKAGLEKLRIKLLFGTSSVYIPVLSPEDARALLLLNETPATGTLLV
                     SKGLSERLEDAASLGYSKVEVSAVLKGLSGVMAASPLPGGTLLTYGTAGEAEKIRRSL
                     LFSQTEMILDNSTFEKSYKQETQFMKLITEIIIYLQFFVVMVIGVVTVIRTVSARRSE
                     ISIRSIIGLKEKETRRMLSAELAVVILSAAAMGCLAGLTVWWIMKSIFFQGVYTSTLV
                     SMPTALRVSLMVLSILVAGFLTVRFFASREDPIAIIER"
     gene            complement(6289..6957)
                     /locus_tag="PLD15_04460"
     CDS             complement(6289..6957)
                     /locus_tag="PLD15_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63187.1"
                     /translation="MFELKDVALLYETRKQALAALKSTTLKIDRGEEIGIYGPSGSGK
                     SSLLFIMSTLKDPSRGEVKYDGKEITALTPSAKAELRRKEFGFVFQEHFLVNHLTVRE
                     NVLLPASDKKDARKRLEEILGFLGLGELQKRFPFELSLGQSQRVAIARALINNPRVIF
                     ADEPTASLDRDNGQKVVKLLRSYCKKNNAILIMVSHSPEMIEGFGRRFKVENGQVSEV
                     TANV"
     gene            7130..7690
                     /locus_tag="PLD15_04465"
     CDS             7130..7690
                     /locus_tag="PLD15_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63188.1"
                     /translation="MIDMDDILTNFNLAFLQIAHRMYEEVPVSVEVKVWDFWKSVPNL
                     TLEMEERVWEAIRSTPNFYESLPAYADRDDLLRLAEIMRAGRHEIYFITSRFPTKGRT
                     VQAQSQRWILEHVGEPGAIIVSSRKGELCQVLGVELAVDDAPHHIENLLDHGINTYIM
                     DWAYNRHIDHRLRVKNLGEFLDCIMD"
     gene            7708..10212
                     /locus_tag="PLD15_04470"
     CDS             7708..10212
                     /locus_tag="PLD15_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB63189.1"
                     /translation="MKRFVLSIFVAAIFLSLASAEPVWEDQIVYFVMIDRFANGDTSN
                     DDMGFGEAGNDNSRYNGGDIQGLIDRLDYIKELGATAVWITPPVANQWWNPWVSYGGY
                     HGYWARDFKRIDEHFGDLELYREFVDKAHEKGLLVIQDIVPNHVGDYFRFIDGQFELN
                     ADSSPTAAPEQYPFSLNNYETDSEKNIYHWTPDISNFNDQLQKFTYQMSGLDDLNTEN
                     PEVIEALKDSYTFWIREAGIDGFRIDTVIYVPYEFWKEFLKGENGIYQVANESGKTGF
                     ITFGEAWVRSDPLSDTGEKVIREFFENGMNSMLDFPLNIELRSIFKEGKPTANLTYRL
                     EKRSEYFDPSRMLTFVDNHDMERFLKGGGLANLKQALAFIFTVPGIPVVYYGTEQGFN
                     ETRAAMFAGGFQSGDVDHYNNNSELFKYIQELITLRKNNPVFRYGTLRVLKDDSNGPG
                     IFAYSLEWEGEKVFVIMNTSGERRILANMDTGMEMCEVVRPLYAFNSLFKDYTVENDG
                     NLVITMNPRSVYVGVATKEKKSVTLPDIEFNVDLEDNQKIDSNYTITGSAKGATSARI
                     IFDTRIEDGKDIEIIDGKWLYEWDISKFDPGIHSILFKVYGQTRRDSIYSKDYKVILD
                     IPEIVLFSGEDPEGDDAGPEGRYLYPTDITFKRQMDLLGVNVRQIGASLVIAMKIKDL
                     TDSWGPQNGFDHVTFQIYIDDPSKKGATVLPFQNASMPDGLDWDYFIFANGWSIVAYS
                     SEGSGSKSFGTAISPTPLVQTNKLTGEVILRIPGETLGRLESFEGLNIYITTWDFDGI
                     EAVYRDIYPQPKGYHFGGGTKEDPYIMDEMLIRLGK"
     gene            10256..10594
                     /locus_tag="PLD15_04475"
     CDS             10256..10594
                     /locus_tag="PLD15_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPB63190.1"
                     /translation="MFFVLIIAVIAVVVAYIVKSFSGDVEEPEESEDIDISFHPHAVA
                     RMQEREIDPERVMELLGGEGRQVKLAHYNRIKVTDGEVTAIVGKGLRKLEVITVYWND
                     GDREDMEEEM"
BASE COUNT         2837 a         2726 c         2738 g         2514 t
ORIGIN      
        1 tactatgttt acataaagcg cgaggttcgc gataaactgg tcaacatgac cgacccaaaa
       61 gccatcgccc aactgctgga tcttctgctc gttgcgaggt ttctcgaaag ggccggcgat
      121 cacgcaacca atatcgccga ggagatctac tacatcgcga aaggcgctaa tctcaaggag
      181 gaaatgcgac gttgagagtt ttaatagtgg acgacgatcc agatattctg aacattatca
      241 gaacggcctg cgaaagcgaa agcatggagg tagataccgc aaccagcgcc gaagaactct
      301 ggcgggcttt acagtcggac agaccggacg ttattcttct ggatatcatg cttcccgatg
      361 ccagcgggct cgatctgata aagaggatac gctcgacaaa cccgaacatt cccataatct
      421 tcctgacggc ccgaaagtcc gatctggaca tgatactggg actggaattg ggggccgacg
      481 attacgttac caaacccttc aatccgcgtg cactggtagc caggataaag gccgttctta
      541 ggcgcacaga agtttccgga aaactcgaag aacaattagt cataggtgag gcgctggtga
      601 atctgaagtc ttacaccgtc acgcgaaacg gggtctcgga agaactcacc agaagggaat
      661 tcgaattgtt gaaaatactg gccgagaacc ccggaagggt cttcacccgg gaagaactac
      721 tcgacaaagt ctggggaatc gatttctacg gggatttcag aaccgtcgat gtccatataa
      781 gcaaactcag agagaaggta ggcgagaagt ttatcaggac agtcagaggc gtggggtaca
      841 aattcgttcc cggtgacgcg aaatgaacga gcctctcctg aacgagctca gcgaagggat
      901 tctcaaggtc gatgagggac tggctattgt cgaggccaac tcttcggcca gaaaactgct
      961 gggagaggtc gagggcaagg cactccccga tggcttgcac gttcagggaa tatctaagat
     1021 cgtggatagt ttcatatcgg agttgaagtt cgagaccgat acgatctatg tcaaagacga
     1081 tatgaccctt tacctgcgtg tgaaggtagt tccgccttac atagtaatga gaaacataac
     1141 aaacgagaag ctcttcgaga acgccaagat ggacttcgtg aactccatag ttcatgaatt
     1201 ctccactccc ctggccgtca taaacggtta cgtccaactt ctaatcgaaa aaagggaaaa
     1261 acttcccgaa gaagtactag agacgattgt aagaatatcc agatcgacta acaggcttgc
     1321 tcgtcttgtg gaagaactgg gtttactctc taacctcgaa ctgcaaaact acagagtgaa
     1381 ggtagagacg gtgaatctga gagaactggt cgatgaagcc ataggcgatc tcgaaagcaa
     1441 atggaagcgg aagaaactgc agatcgaagt atccgtctca ccggacatct atgccgcggc
     1501 cgactctatg cttctcttca gaataatttc caacctcatt tcaaacgccg ttaaatactc
     1561 cagtatcgga gaaaagatcg aaatcacgtc ttacgaaact gaccggaaga tttttctctc
     1621 cgtcaaagac aacggaattg gaatcaagag cgacgaactt cccagggttt tcgagaggtt
     1681 ctaccgcgcc agcaacgcta aaacatcagg ttcctcgggc ctggggctag gtctttcgct
     1741 ggtaaagcac gctttgaacc tgatcggtgg tacgatagat ataaggtctc gctacatgct
     1801 tggaacaacg gtcactataa gcttcccgaa gaagatatga cgccaggagc cgtccttcgg
     1861 aagagcgata agagcgttag gaactgctct ccgtcttcac ctggacgatt atctcgtaat
     1921 tacctgtgga gatgatggag aagtagaagg ttccgctcct gtgaagataa atctctctag
     1981 caccgggtga tgtcgccgtg agcagcgttt cttcgatgct tccgtcgtag gcgttgtaga
     2041 gtgtaagcga gaagaaactt ccctcgtggc tcagatttat cctccattct ccggtcggta
     2101 cggtgaaagg ctcggtaaag tcggtagatg aacccgagaa actcacgatc gtattccact
     2161 gttcgtttgt ggcagttggt gactcttcta caactcgacc cttctccggg gcaaagattt
     2221 cgttgatggt gtaagatttc tcgctttgca ttgcatcatg atcatattta gggcccttct
     2281 ccggttcaag atcataaatc ccgctctcca gtcgcttcgc tcggcccgcg ctgtatatgg
     2341 agacgatcgt tgtgaacctt ctttcacgga ttgtgcttct tttctgcaat ggcccattcg
     2401 ggggtgcttc gtatttacca aaccgctcca gccatttcaa caacgacgat tccctgcagc
     2461 tttccagaag tgccttaccg ttttctagaa gcaggagatt cgcgttgaca taccctgtct
     2521 cgtatcgaag ccatagatat gccggtctat gacttcccac agcgttctgc gaggtttcaa
     2581 aggagatcga gaaatcccga tctcgcagaa gcctgtcaag aaaagaactg tcaagttgct
     2641 ccgtgctcag tccagccata cataaggtta acggttggcc gttatccaga aacatcaacg
     2701 aatttccagc gccaaccaga agaagcggac agcgaaacac ttccctatct ccaggataga
     2761 gatcgaagta ttctctgtag taagagagag gttcaccatc tttgaaggtt accgtcagcg
     2821 gggttagagc gatcaacgaa acagagaccg tcacaatcaa aagaaagacc gttgctttca
     2881 tctatcctcc ctttttttgc tcgtttctct ttcccctctg gataacaaaa tggaaaagca
     2941 tgactcctga cgaaagaaat ttaatgcttt atgactccac ggtaatttgc tgaaatcatt
     3001 atatcatttt tagttaattg aaaacacatc ttacccaatc tgtagaatca cgaagaaaca
     3061 aaatgcatga agatcagtca atgtgtgcca ttttgacggc tggatggcct ggagcctttg
     3121 gcacattcaa acatcgctac aatctctgac ggatgctgtc aagaattgaa ggggtcgcgc
     3181 cacggaagcc cttcatgtcg ggcgatgaaa acatagaatc tagtatggcc tcaaccgtgg
     3241 cccagactga tgctttgaag atctgactca tggttgaacc gtcctctccg atagtcaatg
     3301 cctgaggatc gctcctatcg gctgtggaaa aggccagcgc gatgtcgccg ctgccgtgat
     3361 aaccgggggc tccgagtatt ccgatggaaa gactcaaatg cctggcgacc ctcttcagat
     3421 ggtgaggtag caacggaata tctgtcgaga gaaccattat caatgaaccg ccggtctttt
     3481 ggggttcttc gaagttagcg tcaaccggcc cgggcatctt tagatcggct gtcctcccga
     3541 agttggccag cactagagct cccagggtgt actccctgcc gcctatctcg attaccctgg
     3601 aagaggagcc gatccctccc ttgaatccga aacagatcat ccctgtgccg gcaccgacgc
     3661 tgcccatcgg gaaatcttcc acgactccat cgtaagcctc gatcgtcatc atatccgtca
     3721 ctgagagaga ggcgatgtca ctgaggtaac tatcgttgca ttccatcacg acggcgttga
     3781 aagaagtggt cccggggtgc tctcggagag cgattttgac cgcgccctgt tgaaccgctc
     3841 ctatggatag cgtgttggtc agaaagacat ggctctccat cgtcccgagc tcgtcgacct
     3901 gaaccagccc catagacttt ccgtagccgt tgaaaacgtt acaggcggcc cacaccggtt
     3961 tggttatgac atcgtcgatc ctgagaaccg taacgccggt cctcaggacc gtcggccgat
     4021 ctctgtcgac cgtgaagtgg ccgactctaa ggcgcggcac atcggtgatc ctgttcaact
     4081 tccccgtgga gaaaccatcc gtaagcgagg cgtattctct gtatctcatg tggctacctc
     4141 tctattatcg ctatcggatc ttcccggctt gcaaagaacc taacggtcag gaaaccggcc
     4201 acgagaatcg agagaaccat gagagagact ctgagtgccg tcggcatgct tacgagtgtg
     4261 gaggtataga cgccctggaa gaatatcgat ttcatgatcc accagactgt caagccggca
     4321 aggcatccca tcgccgccgc cgacagtatc actacggcga gctcggccga aagcatccgg
     4381 cgagtctctt tttctttcag cccgattatg cttcttatag atatttccga acgtcttgcg
     4441 cttacggttc ttattacagt taccacgccg atcaccatta ccacgaagaa ctgcaaatag
     4501 atgatgatct ccgttatcag tttcatgaac tgcgtctcct gtttatagga cttttcgaat
     4561 gtcgaattat ccaggatcat ctcagtctgt gaaaaaagaa gacttcttcg aatcttttcg
     4621 gcctctccgg ccgtgccata ggtcagaagc gttccacccg gaagcggtga ggcggccatc
     4681 acaccggaaa gccccttcaa tacggccgat acctcaacct ttgaataccc taacgatgcc
     4741 gcatcttcaa gtctttccga aaggcctttg ctgaccagca acgttcccgt tgccggtgtt
     4801 tcgttcaaca ggagcaaagc ccgggcatct tccggcgaga gcaccggtat atagacactg
     4861 ctcgttccaa agagcagctt tatcctgagc ttttcgagcc cggccttaaa ggacttcact
     4921 tccggggagg acgtaatatc ggatggaaac tccacgccgt cggccatcct cacatatgtt
     4981 ctgttttcga agaagatccc gccgtccctt attccatcgg aaagaccgga aagaagggaa
     5041 ccgaagaaaa atatcaaagc tacgaaaacg accacgttca caaaaagggg cgtataggcg
     5101 ccggttctca ccggataacg caaccagcct gtacgtatat ccctgaaacg actcctgaag
     5161 attccctttt ccctgctggc gctcctcagg agatttatga ggtcgcgttt cagatgaccg
     5221 gttatgagaa actgggtcag caacaccaca gccagcggaa ttattagggt gtagaacgag
     5281 ccgacgggat catttctgag gaaaagcccg gcgggttcga agaacctttt ctcgagaacc
     5341 gagatggtcc atacaccggc gaggaagcct gcgatccatc ctaaggtagc ttgaatcgaa
     5401 gactctatca agagcctgac cttaaggctt ccgcctgtat agccgaccgc ggacataacg
     5461 ccgtactcct ttctccgttc acgtagactt acagagttga gcatggtcag tcccacggta
     5521 aagatcagtg caactaccag attcacgaaa aagactcccg tgtaatagcc ggcgtactgc
     5581 tctcttatcc tgtcgatcga cgaggccggt ccattaaccg taatagacgg catatactcg
     5641 accagcgcgt tcttcaactc gttctcgacg gccatgagaa aaccttctct ggcgaagatc
     5701 atgtagccat aagtgccgcc gcccgaggag attcccgcag ggcctaaacc gaagatcgat
     5761 ggtcctttca atgaagccac gatagaatac tgaccgggtg tggacatctc cagcgtctct
     5821 tcgtagatct ggccgacttc cagtctgtcg gccttgagga aggaaccgga taccattata
     5881 tctctcgagt ctctctcgaa aaccgtaccc cgagctatct gcatctccga ctctcgcaga
     5941 agtctgggaa tatccgtctg tcttatcccg tacaccggga agttggctct ggcgctgccg
     6001 aagaggttgt aactgagata ggcgatatcg acttttatcg cgtcggaaaa gccaaaggtt
     6061 tccgagagcg ttttctcgat cacctcgtcc agccgtgaat ctctgccgtt actggagagg
     6121 tactgaatgg ttatccagct ttcgttgact gcggatatcc tgtttctggt ctcgccaaaa
     6181 gagagaaata tcgaaccgag gatcatgctg gccccgacac ccagggcaac gaccagaata
     6241 aacgccagaa ttctcaacgg cctccttttg aagtataacc aaatatactt aaacattggc
     6301 cgttacctcg ctcacctgac cgttctctac tttgaacctc ctcccgaatc cctctatcat
     6361 ttccggcgag tgagagacca ttataagaat ggcgttgttt ttcttgcagt atgatctgag
     6421 cagcttcaca actttctggc cgttatccct gtcgagcgat gcggtcggct cgtcggcgaa
     6481 aatcactcgt ggattgttga tcaacgcgcg ggctatggcc actctctggc tctgaccgag
     6541 agagagctcg aagggaaatc tcttctgcaa ttcaccaagt ccgaggaaac ccagtatctc
     6601 ctcgaggcgt tttctggcgt ctttcttatc gcttgccgga agtaaaacgt tttccctcac
     6661 cgtaagatgg tttacgagga aatgctcctg aaaaacaaac ccgaactctt tacgtctaag
     6721 ctcggccttc gctgaaggtg tgagcgcggt gatctccttg ccgtcgtact tcacctctcc
     6781 gcgactggga tctttgagcg tgctcattat gaagagaaga gaggatttgc ccgatccaga
     6841 agggccgtag attccgattt cttctccccg atctatcttc aatgtggtcg atttaagtgc
     6901 ggcgagagcc tgtttcctgg tttcgtaaag caacgctacg tccttcagtt cgaacatact
     6961 ctttattcct ccttttaatc cgctgccttt tcctttcaga ttatcccaca aaacaaagcc
     7021 ttctcaaacg gtctttcata atgagtctct tcgccgctta attctactgg ctgctcgata
     7081 acgcgtccgg ttgctatata ctttatatgg aggtgttttt gtgaaggtaa tgatagacat
     7141 ggacgatata ctgaccaact tcaatctggc ctttttgcag atcgcccacc ggatgtacga
     7201 agaagtgccg gtgagcgtcg aggtgaaggt ctgggatttc tggaagagcg ttccaaacct
     7261 cactctggag atggaagaga gagtgtggga agctatccgc agcacaccta acttctacga
     7321 gagccttccg gcctatgccg accgcgacga tttgttaaga ctcgcggaga ttatgagagc
     7381 ggggagacac gagatatact tcataacctc acgcttcccg acgaagggac ggaccgtcca
     7441 ggcacagtca cagagatgga tactggaaca cgtgggagaa cccggcgcga tcatcgtcag
     7501 ctcgcgcaag ggcgaactct gccaggtcct gggagtggaa ctggcggtgg atgatgcgcc
     7561 tcaccacatc gaaaaccttc tcgaccacgg aattaatacc tacataatgg actgggccta
     7621 caacagacat atcgaccaca gactccgggt aaagaacctg ggagagtttc tcgattgtat
     7681 aatggattaa aaataaggag gtgtcctatg aagaggttcg ttctgtccat tttcgtagct
     7741 gccattttct tatccctggc ttcagcagag cctgtatggg aagaccagat cgtgtacttc
     7801 gtcatgatcg acaggttcgc caacggcgat acttccaacg acgacatggg ctttggcgag
     7861 gccggaaacg acaattctcg ctacaacggc ggagatatcc agggattgat agacaggctg
     7921 gattacataa aagagctcgg cgcaacggcc gtatggatca ccccaccggt cgcgaaccag
     7981 tggtggaacc cctgggtgag ctacggggga tatcacggct actgggcgag ggatttcaag
     8041 aggatagacg aacatttcgg agatctggaa ctatacaggg aattcgttga caaagcccac
     8101 gagaagggcc tgctggtcat ccaggatatc gttccaaatc acgtcggcga ttatttcaga
     8161 ttcatagacg ggcagttcga actcaacgcc gatagctccc ccaccgctgc cccggagcag
     8221 tatcccttct cgctaaacaa ttacgagacc gactcggaga agaacatcta ccactggact
     8281 ccggacatct cgaacttcaa cgatcagctc cagaaattta cgtaccagat gtcgggactg
     8341 gacgacctca acacggaaaa ccccgaagtg atagaggccc tgaaagattc ctacaccttc
     8401 tggataaggg aagccggtat cgacggcttc aggatagaca cggttattta cgtcccctac
     8461 gaattctgga aggagtttct caagggagag aacgggatct accaggtggc taacgagagt
     8521 ggcaaaacgg gtttcataac tttcggagag gcctgggtca ggagcgatcc cctaagcgat
     8581 accggtgaga aggttatacg cgaattcttc gaaaacggta tgaactccat gctggacttc
     8641 ccgctgaaca tagagttgag gagtatattc aaagagggaa agcccactgc caacctcaca
     8701 tacaggctgg aaaagagaag cgagtatttc gatccttcca ggatgctcac cttcgtggat
     8761 aaccacgaca tggagaggtt cctcaaagga ggcggcctgg ccaatctgaa acaggcgctg
     8821 gcatttatat tcaccgttcc cggcatccct gtggtctatt acggcactga acagggcttc
     8881 aacgaaacac gcgccgcgat gttcgccgga gggttccagt cgggagatgt ggaccactac
     8941 aacaacaact cggagctttt caaatatata caggaactca ttacactcag gaagaacaac
     9001 ccggtcttcc gctacggaac tctgagggtc ttgaaggacg attccaacgg tccaggtatc
     9061 ttcgcctaca gcctcgagtg ggagggcgag aaggttttcg tcataatgaa cacctccggc
     9121 gagcggagga tactggccaa tatggatacg gggatggaga tgtgcgaggt ggtcagaccg
     9181 ctgtacgcct tcaactctct gttcaaagac tacacagtag agaacgatgg caaccttgtg
     9241 atcacgatga atccccgctc cgtctatgtg ggggtcgcca ccaaagagaa aaagagcgtc
     9301 actctaccgg acatagagtt caacgtcgat cttgaagaca atcagaagat agattcgaac
     9361 tacactataa ccggcagcgc aaaaggagcg acttcggcca gaataatatt cgacacaagg
     9421 atagaagacg gcaaggacat agagataatc gacgggaaat ggctctacga atgggatatc
     9481 tccaagtttg atcccggtat acattctatt ctcttcaaag tttacgggca aaccaggcgt
     9541 gattccatat acagtaaaga ttacaaagtg attctggaca tcccggagat cgttctcttc
     9601 agcggtgaag atcccgaagg cgacgacgcc gggccggaag gcagatatct ttatccgacg
     9661 gatataactt tcaagaggca gatggatctt ctcggagtta atgtgagaca gatcggcgca
     9721 tccctggtga tagctatgaa gataaaggac ctgaccgatt cgtggggacc ccagaacggc
     9781 ttcgatcatg taaccttcca gatctatatc gacgacccgt ccaaaaaagg agcgacggtt
     9841 ctgccgttcc agaacgcatc gatgcccgat ggactcgact gggactactt tattttcgcc
     9901 aacggctggt cgattgtcgc ctactcctca gagggcagtg gatcgaagtc tttcggcacg
     9961 gccatctctc cgacaccgct ggttcaaacg aacaaactca ccggagaggt tatactgaga
    10021 ataccgggcg agactctggg aaggctggag agtttcgaag gtttgaacat ctatataacg
    10081 acctgggatt tcgacggcat agaggccgtt tacagggaca tttacccgca accaaaggga
    10141 taccatttcg ggggagggac aaaagaggat ccgtatataa tggatgaaat gctcatcagg
    10201 ctgggaaaat gataaacgga gaggtctttt ctccgttctg ttcatggtgg tatatatgtt
    10261 cttcgttctt ataatcgcgg taattgcggt cgttgtagcg tatatcgtaa aatctttcag
    10321 tggagacgtc gaggagccgg aagaatccga agatatcgat atctcttttc atccgcacgc
    10381 ggttgccagg atgcaggaga gagagatcga tcctgaaaga gtgatggagc ttttgggcgg
    10441 cgaaggccgg caggtaaaac tggcgcacta caacaggata aaagtgacgg acggggaagt
    10501 aacggccata gtgggcaagg gattgcgaaa actcgaagtc ataaccgttt actggaacga
    10561 cggcgatcga gaagacatgg aagaagagat gtagatcgtc ttgcggatcg agaaaagttc
    10621 gtgagaattg accttaaaga cgatacgagc aacgagggcg aagtctcgat ctgttcggaa
    10681 tatatgtggg ggtaattgtg gaaaatttga agcgggacac tgaagatctg atgaaagctt
    10741 acgcggacct tccgctcgat gattacagaa gaaagctcga ccttctcaac aaactcatgg
    10801 ctcatatgtg cagag
//
LOCUS       DAOOLY010000059        10812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_6897_length_10812_cov_5.084410, whole genome shotgun sequence.
ACCESSION   DAOOLY010000059 DAOOLY010000000
VERSION     DAOOLY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6897_length_10812_cov_5.084410"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1598
                     /locus_tag="PLD15_04480"
     CDS             156..1598
                     /locus_tag="PLD15_04480"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB63191.1"
                     /translation="MDWPEGSLRDIVLDSHNYIDYGNYPDVNIGSTGTLIAQIPQVPH
                     TYQYFHSRYSFMNEKGVAISESTFSIDTSTDYGKEVRKVLWTDSMGIIDCWTAQDIAL
                     ERAATAREAVRVMGDLVEQYGWSTVGGGGETMTVADGEECWIAEFYGRDVWVAVRIPT
                     DHFTVAANRARIMEVDLNDTENVMASPNIVSFAVEQGWYDPASGKPFNIAEIYAPNDN
                     LYATRREWRAFDIVAPSLGLSPHDVRFPLSVKPERLLTVHDIFVIKGDYYQGTEYDLT
                     VGPAAGPWGDPIRYANTSRTGTWERSINMHRTCYVHIGETNSAYADPFKGVAWYGYGA
                     PDTAYIVPLWPIMRDLPKFYETGSRYEEFRRDSGWWVSSYVQQMAELHYNLAIQDIRN
                     YRDPKLEVLYKLTPQVQAIATEMYKTDPEAAIDFVSNYAFMNAVAWFEEWKLLGDRLL
                     GNYALGYVNFRTAAYPDWWNELIGYGFPER"
     gene            1778..2050
                     /locus_tag="PLD15_04485"
     CDS             1778..2050
                     /locus_tag="PLD15_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63192.1"
                     /translation="MQNIEKILDLVEELFDELENTDVQDLFESLPDMEQDEVVELRSS
                     IEDWVDGLDEVISRLPELQSSIVELKADLERLNDSVVELEEDFDEE"
     gene            complement(2091..2714)
                     /gene="wrbA"
                     /locus_tag="PLD15_04490"
     CDS             complement(2091..2714)
                     /gene="wrbA"
                     /locus_tag="PLD15_04490"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HPB63193.1"
                     /translation="MNTTKINVIFHSIHGHIYKLALAEAEGAKSVEGVEVKIYRVPET
                     LSREILEKMKAVESVESFSYLPVADLKTLSEADGLIFGTPTRFGMMTSQMRNYLDSTG
                     SLWSKAILVGKPATVFTSTSTQHGGQEAAILSFHTTLLHHGMILLGLPYVHSEMFEVE
                     KVSGGGPYGASTLTGSDGRRQPSEAELKLAFEQGKYLATIASRLKNK"
     gene            complement(2851..4971)
                     /locus_tag="PLD15_04495"
     CDS             complement(2851..4971)
                     /locus_tag="PLD15_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPB63194.1"
                     /translation="MVIDPRIDQKILDLLSYRFHWKELREIQGKTISPILDGDNILLI
                     AGTASGKTEAVMIPVLNRLLDAEAGLKCIYFAPLKSLINDIASRLEVMLSPFGLFVGK
                     WHGDLSRWDKESALKNSSILVTTPESVEGILSGSNACLFENIEFIIIDEVHAFIESPR
                     GAQLVSILERLRVLSQADPQRIAMSATVGNPDRMMKWLSGSSERRGVIVAEGKKVSRS
                     IEVFTDGEIEPVDYLLKLLKGTGEKILVFSYSRSKAEEFAAIAKSLGLNVPVHHSSVS
                     KSQRGQIEDEFKNNSDLRAVVATSTLEMGIDIGDIDRVVFLELPSSAASFLQRAGRSG
                     RKKGKSKITVFVNDSQSCYNLLGILKKLDENTVEPVEPIDFYPQLLAHQLIGLSYWRG
                     LLSAGDLDFLKRAFPFTRVGRDHFKMLVDHLIEREFLTERDGMIVPGASAAEIFGSGR
                     SKMDFVVLFPGSLEYSVVLSGEEIGRIHPLILNGQEGEGGENSFILGGKSYLVREIDR
                     KERVVHVVPGRGGRLPGWLGGSSAVTKSFARSIMNAMIDLPDYEETLLSEGTHKRLEE
                     ISRDVVADGRIRLISEKEGNTVITYAGDMSNIFLVTCLKAVFGVERTSSNWRDVKIKD
                     KLGTEELADMLLALAKMDHSELKTFLSLFLMGEPGRLRKMYDLFGDKLYDYAPENLIA
                     EFVVRNIFDPGLLKELEDVDCQLI"
     gene            complement(4965..6155)
                     /locus_tag="PLD15_04500"
     CDS             complement(4965..6155)
                     /locus_tag="PLD15_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HPB63195.1"
                     /translation="MDYHPMFEAIRAGAAVPVDYIKEILTGRDKYQVEIENDIDYILT
                     GRSKVRVFLGQYGLGKTTLARYAEYLAREKGMMISSLAEKDYDTLHKQDEFFRSVMKN
                     MSLVGISGNPLKIFLGAWAERIIEELKDQEIPAEEIMPVKEYLSKRDHYDPGGMFSQF
                     CAAYVVSTAKGETTEEFYAYLVGDSIDKRSMKKRGIYHFLQDDGWNFLRSFTSLMDTL
                     QVPGMVVIMDELESIMNRRRDVRERTFNQLREIIDRLSAGFLQRTYFLWLGTDVWFEN
                     KNRGIASYHALYVRMKNITGDDSGKSLILSLKPLELDGFKMLARKLIKLYRECYSFDG
                     GKEIEELLVKSLKQQYTGVDGKIRPTTREVTKLTVEFLDSMRQGRKPESSDKKPVKAV
                     DELW"
     gene            complement(6174..8219)
                     /locus_tag="PLD15_04505"
     CDS             complement(6174..8219)
                     /locus_tag="PLD15_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63196.1"
                     /translation="MSDLKDRGLKGFLSAVDVFEKADESFEGKGWRVIAETQSAAIIA
                     SRAPSIFSIFGKLFEPKFLFLYRGPGGDVLASQSEKLADLSQAVLDAALFQKDFNDSF
                     VDLYSLFKEAPDMFEKTIGHYATHISQGDKDLFSALRELKILYERVKKKFGQYAVKLS
                     LNNLVTSIFKLRCSFLPSIEEDMVDFLEQFPPGPEGMESFIENQILLAAGEPEESEIA
                     VQLSRVETVIHELQKYSSSYLQWFTVPEGVFVSYLEKGMQRIPSLEAWFEGVKDARTR
                     YGLLQTHWEQSRHWYFEKIGAGLIALFQRSMTGQTCWTVVEEKQIKDPLSHAGETVRY
                     SAIRTDFKIVRSDDQEGSYTLCSIDELNVTSSGLKKAAKTFPYENINCNFNELKISKE
                     AFLDSKDARFAGYIWKFLLPGGGQDREAKNNERVECFVYYVITILSKGFGTPLLKLFF
                     AKEETAKLFLEFLKEAAILGSWKEYPQNGLPASLKAKQRTHESDERNIGSQNPDVSVV
                     PAIERFPEILATIDEPEAVEEKKPFTLAGELVEKINSLHYELEWSVKSNRETVLHYYF
                     PEMTTEEVGTIAEEKLPVAGEKTDSLKELENKIEALSGKTLQTVCKILACEPEREYTL
                     EELGLSFWSVFVVAPKITTGGKPLIEVISGEKPRVIWGRNFDSRVKDVLKKKFSFES"
     gene            complement(8300..8788)
                     /locus_tag="PLD15_04510"
     CDS             complement(8300..8788)
                     /locus_tag="PLD15_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63197.1"
                     /translation="MSKREYLRKLLANGRGADEIADLFGLTTEKLQAYLGEEERSRLA
                     LSQVKSDLKRLEEFPLDSLESILLFSFRVILESIGFLFVDLERDLYDSSNDRTSRLIK
                     RLLDGAYLLLAINRLELVGAGIPALRKDTRFSRACQNKAFIAEKLLDIAERIERDLGG
                     DL"
     gene            8921..9868
                     /locus_tag="PLD15_04515"
     CDS             8921..9868
                     /locus_tag="PLD15_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63198.1"
                     /translation="MRKILLAVFLITAIFVSATDYFFGLGFQGLVNVNHTDGEWRISI
                     VPVTSLSSGGVESGGFVFNVLRDRGFALVSRLSLKPVTHVHTFHCEAIDVNAKTLEVF
                     VADGTHGLKIYKFYGPRLYKLVQTLPVKGWVNSLAYHENSILAGTEVDGLFLFKRGPE
                     GFEEIQRLFPSGIDRLEAGITVRAIVASGERFFVAAGSEGALVLEFSDGLLRVTGKLT
                     ANYALDVAVSEKYVAVVEPQESTISFFDVLDLKPITSLDFDASPVQAVIAKNWEDGKD
                     YLLCRFSDSVSIIEMGAFQELFSMKGAFFGIVKPVYGID"
     gene            complement(9865..10716)
                     /locus_tag="PLD15_04520"
     CDS             complement(9865..10716)
                     /locus_tag="PLD15_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63199.1"
                     /translation="MKIIKGQSLLVDVIIWALLIATALLILSPIVFMFTASLMPARDI
                     VKMPYRWIPEELYLDNYAQAIRGNDGSYIYPRNILNSFIVAGLVSVTTVLLSALTGYG
                     LAKFPFKGRNLVFLLIMATMMIPFEAIMIPLYLVATSLNIQNTYIGLILPFLTNAFGI
                     FLMRQYLITFPDEILDAARIDGSGELSIFWRIILPNSAPAVATLAILTFRSQWDNLLW
                     PLLISQSEEMKTIPLYIVKFAMEKHTNEGAMMAAASIASIPILILFITLSKYFVSGAS
                     LYSSRKG"
BASE COUNT         2614 a         2836 c         2565 g         2797 t
ORIGIN      
        1 ggcgctagcg cttccggcac ttatatcaac cgcaatcatc tggcgggtct gctggaaatg
       61 gcgctaccgg taagtctggc gctactggca gttacggtgg ggcaaggccg gccctcgcac
      121 gccagtagca gccggaggca gcgaaccttc cggcaatgga ctggcctgaa ggttcgctga
      181 gagatatcgt cctcgattcc cacaactata tcgattacgg taactatcca gacgttaaca
      241 tcggttccac cggcaccctg atagctcaaa ttccccaggt accgcacacg taccagtatt
      301 tccactcaag atactccttc atgaacgaga agggtgtcgc catcagtgaa tccactttct
      361 ccatcgacac aagcacagat tacggtaagg aagtaagaaa agtcctctgg actgacagca
      421 tgggcataat cgattgctgg acggcacagg atatcgctct tgagagagcg gcaactgcaa
      481 gagaggccgt cagagttatg ggcgatctcg ttgagcagta cggctggagc accgtcggtg
      541 gcggcggaga aactatgacc gttgcagatg gcgaagagtg ctggatagcc gagttctacg
      601 gaagagatgt atgggtcgcg gtcagaattc ccaccgacca cttcacagtg gcggccaaca
      661 gggccaggat catggaagtt gacctcaacg acacagaaaa cgtaatggcc tctccgaaca
      721 tcgtatcctt cgccgttgag cagggctggt acgatcccgc ctccggtaaa ccgttcaaca
      781 tagcggagat ctacgctcca aatgacaacc tttacgcgac aagaagagaa tggagagcct
      841 tcgacattgt ggcaccgtct ctgggtctca gtcctcacga cgtgaggttc ccgctctctg
      901 taaaacccga aagacttctc acagttcacg atatcttcgt tatcaagggc gattactacc
      961 agggtacgga atacgacctt acggtaggac cggccgccgg tccctggggc gatcctatca
     1021 gatacgccaa cacaagtaga accggaacat gggagagaag catcaacatg cacagaacct
     1081 gctacgtcca catcggtgag actaactccg cctatgccga tccattcaag ggcgttgcat
     1141 ggtacggtta cggcgcaccg gataccgcat acatagtccc actttggccc atcatgagag
     1201 acctaccgaa gttctacgaa accggaagca gatatgaaga gttcagaaga gactccggct
     1261 ggtgggtaag ttcctatgtt cagcagatgg ctgagcttca ctacaacctc gccatccagg
     1321 acataagaaa ctacagggat cccaaactcg aagttctcta caagttgaca ccgcaggtcc
     1381 aggcaatagc gaccgagatg tacaagactg atccggaagc tgccatcgac ttcgtttcca
     1441 actacgcgtt catgaacgcg gtagcatggt tcgaagagtg gaaactcctc ggcgacagac
     1501 tcctcggtaa ctacgctctg ggttatgtga atttcagaac cgcagcttac cccgattggt
     1561 ggaacgaact gatcggttac ggttttccag agagataata gatagacgga gcttcaaagg
     1621 cggccgatgg gccgcctttt tcttttggaa attccctggt ttgtggtagt gagtatcgat
     1681 tcattgtcga aaaaaaactg cattccaaac agtagaaaac tcacgggagt tgtgataaca
     1741 tcggtcagta tagaagaacc tgaaaggagt ggcacgggtg cagaacattg aaaagatact
     1801 cgatcttgtc gaagaactgt ttgatgaact tgagaatact gatgttcaag atttattcga
     1861 atcccttcca gacatggaac aggacgaagt cgttgagctc agatcaagca tcgaagattg
     1921 ggttgacggt ctcgacgagg taatctcaag acttcccgaa cttcaatctt cgatagtgga
     1981 actcaaagcc gatctcgaaa gattgaacga ttccgtagtg gagcttgaag aggatttcga
     2041 cgaggaataa attgcatgat gtggtaagga ggcccttcgt ggcctcctta tcatttgttt
     2101 ttgagccttg aagctatcgt ggcaagatac tttccctgtt caaaagccag tttcagttcg
     2161 gcctcgctcg gctgtctcct gccgtcgctt ccggtaaggg tcgatgcgcc gtatggacct
     2221 cctcccgaca ctttctccac ttcgaacatc tcgctgtgaa cgtaaggcag tcccagcaga
     2281 atcataccgt ggtggagcaa cgtggtgtga aaactgagta tggccgcttc ctgaccgccg
     2341 tgctgagtcg atgtcgacgt aaagaccgtc gcgggctttc caaccaggat cgctttcgac
     2401 cagagtgaac cggtggaatc cagatagttg cgcatttgag aggtcatcat accgaatctg
     2461 gtcggagttc caaagataag accgtccgcc tcggatagag tttttaggtc ggcaacgggg
     2521 agatatgaaa aactctccac cgattcgacg gccttcatct tctcgagtat ctccctgctc
     2581 aaggtttccg ggacccggta aatcttcact tctactccct caacggattt tgctccctcc
     2641 gcctcggcca gagccagttt gtatatgtga ccatgtatag agtggaagat cacattaatc
     2701 ttagttgtat tcaaagcaat tacctccaat tgatatccat tatattgtgt acaatatagt
     2761 ataacatcga aaatcgcttt tgtgaagtcg ccttcgagtt tcggctcgct catatcggtt
     2821 agcttgaatt gaccgcttta aagagcgcgg tcagatcaat tgacagtcaa cgtcctcaag
     2881 ttcctttagt agtcccggat cgaagatatt gcgtaccacg aattccgcta ttaaattctc
     2941 cggagcgtaa tcgtagagtt tatcgccgaa cagatcgtac atctttctca gccggcccgg
     3001 ttcccccatt aaaaaaagcg acaggaaagt tttcagctct gaatggtcca tttttgcaag
     3061 cgccagtaac atatcggcca gctcctcggt gccgagttta tcctttatct ttacatctcg
     3121 ccaattcgaa gatgtccgct cgacaccgaa cacggccttc aaacaggtaa cgaggaaaat
     3181 gttggacata tctcccgcgt aagtgattac ggtgtttccc tctttttctg aaatcagcct
     3241 gatccttccg tccgccacga cgtcgcggga gatttcttcc agcctcttgt gggttccctc
     3301 tgatagcagg gtttcttcgt agtcaggaag atcgatcatc gcgttcatta ttgaacgggc
     3361 aaaactcttc gtgaccgccg aagatccacc aagccaaccc ggcagccttc ccccgcgtcc
     3421 gggaaccaca tgaacgactc tctctttccg atcgatttct cttacaaggt aagactttcc
     3481 acccagtatg aacgaattct cgccaccctc gccctcctgt ccattcagta tcagcggatg
     3541 tattctgccg atctcttctc cggaaagcac cacggagtat tccagggaac cgggaaaaag
     3601 aacaacgaaa tccatctttg atcggccact accgaagatt tcggcagcgg aggccccggg
     3661 aacgatcatg ccgtcgcgtt cggtcagaaa ctctctttct attaagtgat caaccagcat
     3721 tttgaagtga tctctgccca ctcgggtgaa gggaaatgct cttttgagga agtccagatc
     3781 cccggcactc aacaagcccc tccagtacga gagtcctatc agctgatgag ccagaagctg
     3841 tggatagaag tcgattggtt ctacaggttc caccgtattt tcgtccagtt tcttgagtat
     3901 tccaaggagg ttgtagcagc tctgactgtc gtttacgaaa accgttattt ttgacttccc
     3961 tttcttacgt cccgaccgtc cggccctctg gagaaaggag gcggccgaag agggaagctc
     4021 gagaaagacc accctgtcga tgtcgcctat atcgattccc atctcgagag tcgaagtcgc
     4081 gaccactgcc ctcaggtcag aattattctt gaactcatct tcgatctggc ccctttgaga
     4141 tttcgataca gagctgtgat gtaccggaac attgagtcct agcgacttcg cgatcgcggc
     4201 gaactcttcg gctttcgatc gggagtaaga aaaaacgagt atcttctctc cggttccctt
     4261 caaaagcttc aacagataat cgacgggttc gatttctccg tccgtgaaga cctctatgct
     4321 cctggaaacc ttcttgcctt cggcgactat cacgccgcgc ctttcggagc ttccgctcag
     4381 ccatttcatc atcctgtccg ggtttcccac agtcgccgac atggctatcc tctgggggtc
     4441 ggcctgcgaa agaactctca atctttccag aatcgacacc agctgcgccc cgcgcggcga
     4501 ttctatgaaa gcgtgaacct cgtcgatgat gatgaactct atattttcga acagacaggc
     4561 atttgaaccc gaaagaatgc cttcaaccga ttcgggggta gttaccagaa tcgaagagtt
     4621 cttaagagca ctttccttgt cccacctgga gagatcgccg tgccacttgc ccacgaagag
     4681 gccgaaagga ctcagcatta cctcgagtct ggaggctatg tcgtttatga gagatttgag
     4741 cggcgcaaaa tatatgcact tcaggccggc ctccgcatcc aacagcctgt tcaacaccgg
     4801 aatcatgaca gcttcggttt tgccagaggc tgtgcccgct attaaaagta tattatcgcc
     4861 atcgagaatc ggcgagatag tcttgccttg aatctctctg agctctttcc agtggaatcg
     4921 ataagagagg agatccagaa tcttctggtc aattctcgga tcgattacca gagttcatct
     4981 accgccttca ccggcttttt atcagaagac tccggttttc gaccctgacg catactatcg
     5041 aggaactcaa ctgtcagttt ggtcacttct ctcgtggtgg gtcttatttt gccatccact
     5101 ccggtgtact gctgtttaag agacttcacg agcagctctt cgatttcctt tccaccgtcg
     5161 aacgaatagc attctctgta aagcttgatc agttttcttg ccagcatctt aaatccatcg
     5221 agttcaagag gcttgagcga aagaatcagg gactttccgc tgtcatcgcc cgtgatgttc
     5281 ttcatcctga cgtacagagc gtggtacgag gctattcccc tgtttttgtt ctcgaaccat
     5341 acatccgttc ccagccacag gaagtaggtg cgttgaagga atccggccga aagtctgtcg
     5401 attatctctc ttaactggtt gaaagtgcgc tctctaacat cgcgcctcct attcattatg
     5461 ctctcgagtt catccattat aactaccatc ccgggaacct gtagcgtgtc catgagggaa
     5521 gtgaaggatc tcaggaaatt ccatccgtcg tcctgaagaa aatggtagat accacgtttt
     5581 ttcatggatc ttttgtcaat gctgtcaccg acgagatagg cgtaaaactc ctctgttgtc
     5641 tcgccttttg ccgtgctaac cacataagct gcgcagaact gcgagaacat tccgccggga
     5701 tcgtagtggt ctctcttcga aaggtactcc ttcacaggca ttatctcttc ggccggtatt
     5761 tcctgatcct tcaactcttc gatgatcctc tcagcccagg cgcccagaaa gattttcagg
     5821 ggatttccgc ttataccgac cagagacata tttttcatga cagacctgaa gaactcgtcc
     5881 tgcttgtgta gtgtgtcgta atccttttcg gcaagactgg agatcatcat ccctttctct
     5941 ctggccaggt actcggcata tctggcgagg gtggtctttc ccagcccgta ctggcccaga
     6001 aagactcgta ctttgctcct gcctgtgagt atatagtcga tatcgttttc tatctcgacc
     6061 tgatacttgt ctctacccgt gaggatctcc tttatataat cgaccggaac ggccgctccg
     6121 gcccttatcg cttcgaacat cggatggtaa tccactggta gctctccttt cgcttagctt
     6181 tcaaaagaga atttcttctt gagaacgtcc ttaaccctgg aatcgaagtt acgaccccat
     6241 atgacccggg gcttctcgcc gctaataacc tcgatcagag gctttccacc ggttgtaatc
     6301 tttggggcga ctacgaaaac gctccagaag cttagaccga gttcttcgag tgtgtactcc
     6361 cgttccggtt cacacgccag aattttgcaa accgtctgta gcgtctttcc cgaaagcgcc
     6421 tctatcttgt tctcaagttc cttcagacta tccgttttct ctccggctac cggcagcttt
     6481 tcttcagcga ttgttccgac ctcttctgtg gtcatttcgg ggaagtaata atgcaggacg
     6541 gtttcacgat tgcttttcac ggaccactcc aattcataat ggagagagtt tatcttctcc
     6601 accagttccc cggctagcgt gaatggcttc ttctcttcga ccgcttccgg ctcatcgatc
     6661 gtggcaagga tctcgggaaa cctctcgatt gccggcacga ccgatacatc agggttctga
     6721 cttccgatgt ttctctcgtc cgattcgtga gtcctctgct tggctttcag agaggctgga
     6781 agtccgttct gcggatactc cttccagctt cccagtatgg cggcctcctt caaaaattcc
     6841 aggaacagct ttgcagtttc ctctttcgcg aagaaaagct tcagaagcgg tgtgccgaag
     6901 ccctttgaca gtatcgtaat cacataatat acgaagcatt caactctttc gttgtttttg
     6961 gcctctctgt cctgaccgcc accgggaaga agaaatttcc agatgtatcc ggcgaacctg
     7021 gcatctttgc tgtcaagaaa ggcctctttc gaaattttca gctcgttgaa attgcagttt
     7081 atgttttcat atggaaaggt cttcgccgct ttcttcaggc cactggaagt tacgttcaat
     7141 tcatctatgg aacagagcgt gtaggaacct tcctgatcgt cgctgcgcac tattttgaaa
     7201 tcggtcctta tcgccgaata acgcaccgtc tcgccggcgt gggagagggg atcctttatc
     7261 tgtttctcct ccactactgt ccagcacgtc tggccagtca tggatctttg aaacaacgct
     7321 atcaaaccgg ctcctatctt ttcaaagtac caatgtcgcg attgttccca gtgagtttgc
     7381 aaaagaccgt accttgtcct tgcatccttt acaccctcga accacgcctc cagcgaagga
     7441 atcctctgca ttcccttctc cagatacgat acgaagactc cttccggtac agtgaaccac
     7501 tgtaggtagc tcgaggagta cttctgcagt tcgtgaatca ccgtttcaac tctggaaagc
     7561 tggacggcta tttcgctttc ttccggttct cctgccgcca ggaggatctg gttttctatg
     7621 aagctttcca ttccctccgg tccgggcggg aattgctcga gaaaatccac catatcttcc
     7681 tcgatcgatg gcaggaagga acagcgcagc ttgaagatcg atgtaaccag gttgttcaaa
     7741 gaaagcttaa cggcgtactg gccgaacttc ttcttcactc tctcgtagag aatcttcagc
     7801 tctctcaaag cggaaaacag atccttatct ccctgcgaga tgtgcgtagc gtagtggccg
     7861 atggtcttct cgaacatgtc cggcgcttct ttgaaaagcg agtaaagatc tacgaaactg
     7921 tcgttgaaat ccttctgaaa cagggctgca tcgagaacgg cctgcgagag atcggcaagc
     7981 ttttccgact gagaagcaag gacatcgccc cctggtcccc tgtatagaaa aaggaatttc
     8041 ggctcgaaca acttgccgaa aatcgagaaa atggacggag ccctggaagc tataattgct
     8101 gcagactgag tttcggctat cactctccag cctttcccct cgaaactctc atcggctttt
     8161 tcaaaaacat caaccgccga aagaaacccc tttaacccgc gatcttttaa atcagacaag
     8221 actgtccctc ttttcgatca tcatccaggt tgattatact acacttatct ttgccgcttt
     8281 cgaacaactc ccacaacact cacaggtctc cgccaagatc tcgctcgatt ctctcggcta
     8341 tatctagcag tttttccgca ataaaggctt tgttctggca ggctctagaa aatctggtgt
     8401 ctttgcgcag agcgggaatg cccgcgccca ctaactcaag cctgtttatg gccagaagga
     8461 gataggctcc gtcaagaaga cgctttatca accttgaggt tctatcgttc gaagaatcgt
     8521 agagatcgcg ctcgaggtct acgaagagaa aaccgatgga ttcgagaatc actctgaaag
     8581 agaaaagcaa aatgctctca aggctatcca agggaaattc ttcgagcctt ttcaagtcag
     8641 acttcacctg cgataatgcg agtctggatc gttcctcctc acccaggtaa gcctggagtt
     8701 tttcggtggt gagaccgaaa agatcggcta tctcatcggc cccgcgtcca ttggccagaa
     8761 gtttgcggag atactctctc ttgctcattg tatcaccccg atacaattat atctctcaag
     8821 tcgtcaggtt tcagcgacca gagtaaggtt gagaccacca ctctcgaaag cgaatacttc
     8881 gatagtggtt ttgtaataga ctatagacgg aggtggttct atgagaaaaa ttctgctggc
     8941 tgtctttctc attaccgcca tttttgtatc ggcaacggat tatttcttcg ggcttggctt
     9001 tcagggcctg gtcaatgtga atcacacgga cggtgaatgg cgcatcagta tagtgcctgt
     9061 cacatccctt tcaagcggcg gtgtcgagtc tggcggcttc gttttcaatg tgttgagaga
     9121 tcggggcttc gccttggtgt cgaggttatc cctgaaaccg gttacacatg tgcacacttt
     9181 ccactgcgag gcgatagatg ttaacgcaaa gacgctcgag gtctttgtgg ccgacggaac
     9241 acacggcttg aaaatctaca aattctacgg cccacgcctt tacaagctgg ttcagaccct
     9301 gccggtcaaa ggctgggtga actccctagc ctatcacgaa aactccatac tcgcgggaac
     9361 ggaagttgac ggacttttcc ttttcaagcg tggccctgaa ggattcgaag agattcagcg
     9421 actctttcct tcgggaatcg acaggttgga agcgggtatt actgtacgtg cgatcgtcgc
     9481 ttccggcgaa aggttcttcg tcgccgccgg aagtgaaggc gcactggtgc tcgagttttc
     9541 cgacgggctt cttcgagtaa cgggaaaact gactgccaat tacgcgctcg atgttgctgt
     9601 tagcgaaaaa tacgtcgccg ttgtggagcc tcaggaaagt acgatatctt tcttcgacgt
     9661 tctcgatctc aaaccgatca catcgctcga tttcgacgct tcgccggttc aagcggtaat
     9721 tgcaaaaaac tgggaagacg gtaaagatta tctcctttgt aggttcagcg attcggtgtc
     9781 tattatcgaa atgggcgcct tccaggaact tttcagtatg aaaggcgcct tcttcggtat
     9841 agtgaaacct gtgtacggta tagattaacc ctttctagac gaatagagag aggcgccact
     9901 cacaaagtac ttcgaaagag ttatgaagag tatcagaatg gggatactcg cgattgaagc
     9961 cgccgccatc atggctccct cgttggtatg tttctccatg gcgaacttga ctatgtacag
    10021 aggtatcgtc ttcatctcct cgctctggga tatcaacagc ggccagagca agttgtccca
    10081 ctggctcctg aaggttagga tcgcgagagt ggccacggcc ggcgcgctgt tgggcagtat
    10141 tatcctccag aaaatgctca gttctcccga tccatctatt ctcgccgcat ccagtatctc
    10201 gtcggggaag gttatgagat actgcctcat caagaagata ccgaaagcat tggtcaaaaa
    10261 cggcaatatc agtcctatgt aggtattctg gatgttcaac gaagtggcca ccagataaag
    10321 cggtatcatg atcgcctcga agggtatcat cattgtcgcc atgatcagta aaaagacgag
    10381 atttcttccc ttgaatggga acttcgccag tccatagccg gtgagggccg acagcaagac
    10441 ggtcgtgacc gacaccagac ctgccacgat gaaggagttc aatatattac gggggtatat
    10501 gtagcttccg tcgttccctc ttatcgcttg tgcgtagttg tcaaggtaca gctcttcggg
    10561 tatccagcga taaggcatct tgactatatc cctggcgggc atcaacgaag cggtgaacat
    10621 gaaaactatg ggagatagta tcaacagtgc ggtggctatg agaagggccc aaattattac
    10681 atccacaagc aaagattgtc ctttaattat cttcacaatc cacctcacat ataatctact
    10741 tctccacccg acttgctggc tttgaattga agccaggtga agaaaagcat tatgataaag
    10801 agaatgatac tc
//
LOCUS       DAOOLY010000060        10682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7020_length_10682_cov_5.415357, whole genome shotgun sequence.
ACCESSION   DAOOLY010000060 DAOOLY010000000
VERSION     DAOOLY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7020_length_10682_cov_5.415357"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLD15_04525"
     CDS             complement(<1..600)
                     /locus_tag="PLD15_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB63200.1"
                     /translation="MRVCLEWLSDFIDIGDRSVENISRSLSLSGTEVERIEYPWAGLE
                     GAVVGRIESVSVHPAGDRLLVTGVNTGERTLSIVTSDTTVKAGETVAVLPEGSLFDGK
                     AIAAREFSGVRSEGMFLSLQELELEERSLHIMRLECARVGEDLKSLLRLDSPVIEVEL
                     TANRGDCLSMIGLAREVGAVLGLKTGRPPVENDIDTGEDP"
     gene            complement(597..1631)
                     /gene="pheS"
                     /locus_tag="PLD15_04530"
     CDS             complement(597..1631)
                     /gene="pheS"
                     /locus_tag="PLD15_04530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB63201.1"
                     /translation="MFNGLDAGLEEVLKRIMETASLNDLQEVKARYLGKQGLVTSLMK
                     NIKEISPEKRKEYGREVNELKNSIEEAIDNRKNELEELARLARESAEKPDCTVPGKKR
                     DIGSYHLITQVMEEVQDIWVSMGFEVTTGPEIETSYYNFEALNTPEWHPARDMHDTFY
                     TCDGQLLRTHTSPVQIRVLESRKPPLAIFSPGTCYRKDTPDTTHLPMFHQFEVLLVDK
                     GTSVSHLKGTLNHFAKRLFGADRRIRLRPSFFPFTEPSFEVDVSCGICGGQGCRSCRG
                     SGWLEILGAGMVHPNVFKNVGYDPEVWSGFALGTGLERIAMLKYDLEDIRDLLRNDRR
                     FLKGYRRAII"
     gene            complement(1658..2083)
                     /locus_tag="PLD15_04535"
     CDS             complement(1658..2083)
                     /locus_tag="PLD15_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPB63202.1"
                     /translation="MEIEETIALLKSRGYRITPQRVAILRILKDNTNHPGVEDVFRSV
                     IKIYPNISMATVYNVMEVLEREGIIKEIAVSNVSRRYDPDVNPHGHFICRVCEKVFDV
                     SFSNMRSLGKYSPRELDEFVIESLELIYRGVCKDCKDKT"
     gene            complement(2202..3083)
                     /locus_tag="PLD15_04540"
     CDS             complement(2202..3083)
                     /locus_tag="PLD15_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63203.1"
                     /translation="MKKLSILIVFLLLVGLAFGKDAVEELGKLINNLKNSSYEVERFV
                     QESGIITLARSEKVVKSGPYKLVTSYSSIKGEFGWVRNNNGQFAVFSQEEVAFEPLTN
                     LKDSEDNLIDLFNNKDYTVSLFLDLPNSTKVTLSSGGLTMEVTFDSGYRLLKLVKSYP
                     FHTVSVSYRNYSDLSGEGLARLTNATRGMSLVRPPVYLSEAMMEEYFSWSSMDFATDG
                     RSYLYTLLMYSPSYGKMILYFSFNSEPENLQQFSIKMAESNGFSYALEKISSTSALSL
                     LGMVDSATLRKILDSLH"
     gene            complement(3123..3614)
                     /locus_tag="PLD15_04545"
     CDS             complement(3123..3614)
                     /locus_tag="PLD15_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63204.1"
                     /translation="MDNERLVRIMDREIPVEELRVEEKKELKEFAKALVMFKEKCCYK
                     PSDQSRERAVAKIKKIKTKPYKMLGAVAACMIVGLFSVNFFTNNTILVKKKGDLYQLA
                     NVLGYGSGLNERLKVNLLATADTQNQNSVAMMESEMERSLGFLSSRPEQNDFEVSGIN
                     TTY"
     gene            complement(3607..4167)
                     /locus_tag="PLD15_04550"
     CDS             complement(3607..4167)
                     /locus_tag="PLD15_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB63205.1"
                     /translation="MDEKKLIDGLKKKKVWAYEVLYDDYAPRLGSVIKSYLGYDDVED
                     VVQEVFIKVFRNVRKFRGDAKLSTWLYRIAVNVCKDTLSKRKRNNEFLTDFLESEDKV
                     SLEPPASTNVFREVMDEMSFEELMNVVSKLSEDDRLLIKLRDIDDLSYEEIADILDKP
                     VGTVKSRLHYARKRLKELLEEVGYIG"
     gene            4393..>5159
                     /locus_tag="PLD15_04555"
     CDS             4393..>5159
                     /locus_tag="PLD15_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730937.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB63206.1"
                     /translation="MKKEEAKKLFEQLTYKRKNSWLSLDGETVFAYAKGYMKFIDRAK
                     TERLAVDCLVEYLEEKGFKPLESLDRVGKGERVYITRDGKSLVAAVLGENLADGIDMV
                     AAHLDAPRLDLKPYPLVEDSELAILKTHYYGGVKKYQWLNIPLAFVGTVVKKDGTVID
                     VSIGLDREDPVFVISDLLPHLDGREGDFRKVFQGKDLNALSASIPVIEQCEIENPVKL
                     TFLKLLNEKYGMVEEDFYSADVQLVPAFEAREVGLDRS"
     assembly_gap    5160..5259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5260..5622
                     /locus_tag="PLD15_04560"
     CDS             <5260..5622
                     /locus_tag="PLD15_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730937.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPB63207.1"
                     /translation="PTFKDVYDPVNTAKLGYGIAISKYTGSRGKSSTSEASAEFVGEV
                     RALFNRKGIHWQNALLGEVDKGGGGTVAKFFAEKGVTVLDAGVPVLGMHSPYEIISKA
                     DLYETYRAYRAFLEGDDA"
     gene            5615..6259
                     /gene="nth"
                     /locus_tag="PLD15_04565"
     CDS             5615..6259
                     /gene="nth"
                     /locus_tag="PLD15_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPB63208.1"
                     /translation="MPEDCSPDAVSGWMIENFKHDHGYREPFKVLISTILSQRTRDEN
                     TDEASRKLFSVFPTMESIAAARPEELYELIKQSGMYRQKAERIVNCAEILLERFGGRV
                     PDTIEELTSIPGVGRKTANIVLNISFGKEALAVDTHVHRISNRLGWVKTKSPEETEFA
                     LMKIISPHLWGPVNGSMVEFGRQICRPVGPRCEDCAISRCCDYYGRISKEDIPQ"
     gene            complement(6204..7754)
                     /locus_tag="PLD15_04570"
     CDS             complement(6204..7754)
                     /locus_tag="PLD15_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB63209.1"
                     /translation="MLVAAAILLPLVVSFGFSGLDSLGEAVSSPSFAGITLFTLRQAA
                     LSTSLALMAGLPAAIYFSRSRSRLSKLLGLTTYIPFFFPGISMAIGFLAIYGRNGLLN
                     SALALFGFERLQILYSFGAILLGHVFYNAPIVILILGNALKRIPSDLLETARIDGAGK
                     LTRFLKVELPLSVPALVNSALLIFSYCFTSFAVVLILGGAQFATLEVSIYMYFRLLGR
                     PQMAVALSVIQFAFIMLFGVLISISERRIPFEQGEQYIEKKTATRLYSLLFLVFEWVP
                     ILGAFTSSFYDRARGEFIFGRVAGLFKGELPLIGTSLARTVFNSLSLSTVASVTTVAI
                     TFYLAWTARKIRGGARQLKIVSLLALSVTPSMMAISYLTTIDFLPVPVLMLLLYSLVA
                     LPVSLNYINGQVQNAELDFLEAATLDGASRAAKLRYMILPFFKNTLFYAGTVVFAISM
                     GEFGGSLILGAQDFPTFAVAIYRLNGSRYLLEARFLSSMLGLMVISAVAIAGYLLSRS
                     GRSNRSSG"
     gene            complement(7774..8745)
                     /locus_tag="PLD15_04575"
     CDS             complement(7774..8745)
                     /locus_tag="PLD15_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB63210.1"
                     /translation="MKALSLLLLVLLASTGLSMLVVYSYDSFVPLALETFEEFTLKTG
                     IEVKLKTLSDSGSLVNYIVSMGEKFDGDVVIGIDNLLSRKAFEADIFLPYKPAGSENL
                     ISQDLIFDPLWRLTPYDYGAIALIYDSQSITSKIESLQDLTDGKFKKALILQDPRTSS
                     TGLAFLSWTYLLFGEDFEQFWRALKPSILTITLGWDDSFEKFEAGEAPIMVSYATDGA
                     YSAHYYGQSLYSVLIPQGKGYVQIEGAGIVRWTDNPEEAGMFLDFLLSPDFQRHVPLN
                     QWMFPVIEVEMPAVFQYAVKPSTILKLPPEVELEELIGKWERIIYEK"
     regulatory      complement(8779..8880)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8927..10162
                     /locus_tag="PLD15_04580"
     CDS             8927..10162
                     /locus_tag="PLD15_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HPB63211.1"
                     /translation="MNHLKAVILITGDEITKGIVKDTSGGYLAESLTAMGYEVQSIVT
                     VQDDAQCMIREMKGALERADLLVITGGLGSTFDDITLQTVARYAGRPLIYDGDYHGSL
                     ERSFESRFRRKAPADLERQAYTVEGSTILPNAAGSARGCFLKMGAGEIVVLPGPPMEM
                     KAVFAEAIKHMSRPAAPFTRTVGLVDITEPEIEKTLEELLDRFVNVKFVTRVKYYTGP
                     TVILSGPNGEQLEEIIVLLREKYGENIYTTIGEELADVVVKMLVERKKTVSTAESCSG
                     GRLSAILTSVPGVSAIFPGGIVAYSNEVKTSLLSVRRETLESFGAVSEETAGEMARGV
                     AAVFGTDIGLSVSGIAGPSGGSIEKPVGLVCTAISFGKRSWSYTDRFRGDRETIQLRA
                     AHTIFKRLWRILWKEDYTG"
     gene            10138..>10682
                     /locus_tag="PLD15_04585"
     CDS             10138..>10682
                     /locus_tag="PLD15_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPB63212.1"
                     /translation="MERRLYRVNDPVSWIEKNLSSFDRVLVIFPTERMLESFAAVHAR
                     KEEEGYFFSHDVFPFEEIGTSPRIRAERVSLLYNLSLGRLKKVFGSFHGLLRKTVPLD
                     VFGELTTAIRTGGELEVDEENLVRMGYCRTFNVTVPGEFSIKGSITDIFVPGNGHPLR
                     IETFDREIESIRFFDPSTQKSV"
BASE COUNT         2500 a         2829 c         2833 g         2420 t
ORIGIN      
        1 cggatcctct ccggtgtcga tatcgttttc gaccggaggt cttccggtct tcaaacccag
       61 tacggccccc acctctctgg ccaggcctat catggacaga cagtcgcccc ggttggccgt
      121 cagttctacc tcgatcaccg gagagtctag cctgagcagg gatttaagat cctctccgac
      181 ccttgcgcat tccagtctca ttatgtgaag cgacctctcc tcgagttcga gctcctgaag
      241 agagaggaac atcccttcgg acctgacgcc ggagaactct ctggccgcga tcgcctttcc
      301 gtcgaagagc gaaccctcgg gaaggacggc caccgtctct ccggccttca cggtcgtatc
      361 ggaggtgact atggacaacg ttctttcccc ggtgttcacc ccggtcacga ggagcctgtc
      421 accggcggga tggacggaga cggactctat tcttccgacg acggcccctt ccagtcctgc
      481 ccacggatac tcgatcctct cgacctccgt accgctcaaa gacagcgagc ggcttatatt
      541 ctcgacagat ctatcgccaa tatcaatgaa gtcacttagc cactcaaggc acaccctcat
      601 atgatcgccc tcctgtagcc tttcaggaat ctacggtcgt ttctcagcag gtctcttata
      661 tcctcgagat cgtacttcaa catcgcgatc ctctccagcc ccgttcccag agcgaatccg
      721 ctccagactt cgggatcgta acccacgttt ttgaagacgt tcggatggac cattccggcg
      781 ccgagaatct ccagccagcc cgagcccctg caactcctgc aaccctggcc gccgcatata
      841 ccgcagctga cgtccacctc gaagctcggt tcggtgaagg ggaagaagct cggtctgagt
      901 ctgatcctcc tgtcggcccc gaaaagcctt ttggcgaagt gattcagggt ccccttgaga
      961 tggctcaccg aggtcccctt atcgacgagg agcacctcga actggtggaa catggggaga
     1021 tgggtggtat cgggcgtatc cttgcggtag caggttcccg gagagaatat ggccagcgga
     1081 ggctttcgcg actccaggac tctgatctgc accggagagg tgtgtgttct gagtagctgg
     1141 ccgtcgcagg tgtagaaagt atcgtgcatg tccctcgccg gatgccattc gggagtgttc
     1201 aaagcctcga agttgtagta agaggtctcg atctccggac cggtggtgac ttcgaaaccc
     1261 atgctgaccc agatatcctg aacctcttcc atcacctgag tgatcagatg gtaggagccg
     1321 atgtctcttt tctttcccgg cacagtgcaa tcgggtttct cggcactctc tctggcaagt
     1381 cttgcaagct cttccagctc gttttttctg ttgtctatgg cctcttctat ggaattcttg
     1441 agttcgttca cctctctgcc gtattccttt cgcttttcgg gagagatttc cttgatattt
     1501 ttcatcagag aagtgaccag tccctgcttc cccagatacc gggccttcac ttcctgaaga
     1561 tcgttcaggc tggccgtttc cattatccgt ttgaggactt cttcaagtcc cgcgtcgagc
     1621 ccgttgaaca tatcgcacct ccggagttga ctggctttca ggtcttgtct ttgcagtctt
     1681 tgcagactcc tctgtatatc agttcaagcg attcaatcac aaactcgtcg agttcgcgag
     1741 gagaatactt tcccagactg cgcatgtttg agaaagatac atcgaagact ttctcgcaaa
     1801 ccctgcatat gaagtgaccg tgaggattca cgtccggatc gtaacgtcgg gagacgttgc
     1861 tgacggctat ttctttgatt atgccttccc tttctagcac ttccatgacg ttataaacgg
     1921 tagccatgga gatgtttgga tagattttta taaccgatct gaagacatct tctacgccag
     1981 ggtggttggt gttgtctttc agtatcctca gaatcgctac cctctgagga gttatcctgt
     2041 agcctctact cttcagaaga gctattgtct cttcaatttc catcagtcag gacctccaaa
     2101 aacatgtttt attgattata caataaaaac tgagagatta tgagaaagct tcttgaaaag
     2161 ctggtttatc gtgtggtaca tcggggtgtt gccggagttt ttcagtgaag gctgtccaga
     2221 atctttctta gcgtggccga atcgaccata cccagaaggc tcagggccga tgtgctagag
     2281 atcttttcga gagcgtaact gaagccgttg ctttcggcca tttttatgga aaactgctgc
     2341 agattttccg gttcgctgtt gaaagagaag tacagtatca ttttcccgta actgggagag
     2401 tacataagaa gggtgtacag ataagacctg ccgtcggtcg cgaagtccat ggaggaccac
     2461 gaaaaatact cctccatcat cgcctcggag agatagaccg gaggccttac cagactcatg
     2521 cctctggtcg cgttggtgag ccttgccagt ccctcgccgg aaagatcgga gtagttgcga
     2581 tagctcacac ttactgtgtg aaaggggtag gattttacga gcttcaagag gcgatagccg
     2641 ctgtcgaaag tgacttccat ggtcaacccg ccagaggaaa gggtgacttt ggtactgttg
     2701 ggaagatcaa gaaacagtga gacggtgtaa tctttgttgt tgaagagatc tatcagattg
     2761 tcctcggaat ctttgaggtt ggtcaaaggc tcgaaggcca cctcttcttg cgagaatacc
     2821 gcgaactggc cgttgttgtt tctcacccag ccgaattcac ctttgatcga ggaataggaa
     2881 gtgaccagct tgtacggacc ggatttcact accttttcgc tccttgccag cgttattatg
     2941 ccgctctcct gaacaaacct ttcgacttcg tatgaagagt tcttcagatt gttgatcagt
     3001 ttgcccagct cttcaaccgc gtccttacca aaggccagcc caaccagcaa caaaaaaact
     3061 atcaggatgg acagcttttt catggcgatt tcactccttc tgtgtccgag ggtgtgtcta
     3121 gttcaataag tggtgttaat acccgaaact tcaaaatcgt tctgttcggg cctggaactc
     3181 agaaaaccca gagatctttc catctcgctc tccatcatag cgaccgagtt ttgattctgc
     3241 gtgtcggccg tcgccagaag atttaccttc aacctctcgt taaggccgct gccgtagccg
     3301 agtacgttgg ccagctgata gaggtctccc ttcttcttca caaggatcgt gttgttggtg
     3361 aagaagttga cggagaagag gcccacgatc atgcaggccg ccacggctcc cagcatcttg
     3421 taaggcttcg tcttaatttt cttgattttc gctacggccc tttccctgga ctggtcggaa
     3481 ggtttgtagc agcatttttc cttgaacatc accagggcct tcgcgaactc ttttaattcc
     3541 tttttctctt ccaccctcag ttcctcgacc gggatctctc gatccataat acgtacaagt
     3601 ctttcattat ccaatgtacc cgacctcctc gagaagctct ttaagacgct ttcgggcata
     3661 gtgtaatctg ctcttgacgg ttcctaccgg tttgtccagt atatcggcga tctcttcgta
     3721 tgaaagatcg tcgatgtctc taagttttat aagcaacctg tcgtcttcag agagtttcga
     3781 aacgacgttc atgagctctt cgaaagacat ctcgtccatg acttccctga aaacgttggt
     3841 ggatgccggc ggttcgagcg atactttatc ctcgctctct agaaagtccg tgagaaactc
     3901 gttgtttctc tttcttttgg acagagtgtc tttacaaacg ttgacggcta ttctgtacaa
     3961 ccaggtcgag agtttggcgt cgccgcgaaa ctttcgaacg tttctgaaaa ccttgatgaa
     4021 gacctcctga actacgtctt caacatcgtc gtaaccgaga taagacttaa tgacactacc
     4081 cagtctggga gcgtaatcgt cgtacagaac ctcgtatgcc caaacttttt tcttcttcag
     4141 accgtctatt aacttcttct cgtccaacca gaccacccta atctgtctta tatgacatcg
     4201 caaagacgtt cggggttcaa acaatctcat accaattata ctcatctttc gccggaaagt
     4261 cgaaggaaac gtccggagag tttattgatc aaacgatgtg tgatgaaatt gcgggagctt
     4321 tcggctaaaa tctatttgaa gatttccaaa gggtatagat aattcattgt aatttcttgc
     4381 ggaggtgcga ttttgaaaaa agaggaagcg aagaaactct tcgaacaact gacgtacaag
     4441 agaaagaact cgtggctatc gctcgacggc gaaactgtct tcgcttacgc gaaggggtat
     4501 atgaaattca tcgacagggc gaaaaccgag agactggccg tcgattgcct ggttgaatat
     4561 ctcgaggaaa agggcttcaa accgctggaa agcctcgacc gggtcgggaa gggcgagcgt
     4621 gtctatataa ccagggatgg aaagtctctg gtggccgccg tcctgggtga gaacctcgcc
     4681 gacgggatcg acatggtcgc ggcccatctc gacgccccca gactcgatct gaaaccctat
     4741 cctctggtcg aggattccga gctggcgatt ttgaagacac attactacgg cggcgtgaaa
     4801 aagtatcagt ggctgaacat tcccctcgcc ttcgtgggaa cggtcgtgaa gaaggacgga
     4861 actgtaatcg acgtgtcgat aggcctggac agggaggacc cggtcttcgt catatccgat
     4921 ctgcttccgc acctggacgg cagagagggg gatttcagga aagtcttcca gggcaaggat
     4981 ctgaacgccc tttcggcttc cattcctgtc atagaacagt gcgagatcga aaaccccgtc
     5041 aagttgactt tcctgaagct cctcaacgag aagtacggaa tggtcgagga ggacttctac
     5101 tcggccgacg tgcagctcgt gccggccttc gaggccagag aggtaggtct tgacagatcn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ccaccttcaa ggatgtttac
     5281 gatccggtca acaccgccaa actcggttac ggcatcgcca tatccaagta caccggctca
     5341 agaggaaagt cgtcgaccag cgaggcgagc gccgagttcg tcggcgaggt gagggcactg
     5401 ttcaacagaa agggaataca ctggcagaac gctctgctgg gcgaggtcga caagggcggc
     5461 ggcggtacgg tggccaagtt cttcgccgaa aagggcgtca ccgtgctaga tgccggcgtt
     5521 ccggtactgg gaatgcactc gccgtacgag ataatctcca aggccgatct ctacgaaact
     5581 tacagggcct acagggcctt tctggagggg gacgatgcct gaggactgtt ccccggacgc
     5641 cgtctccggc tggatgatcg aaaacttcaa gcacgatcac ggttacaggg aaccgttcaa
     5701 agttctgata agcaccatac tgagccagag aacgcgggac gagaacaccg acgaagcttc
     5761 caggaagctc ttttcggtct tcccgaccat ggaatcgatc gccgcggcca ggccggagga
     5821 gctgtacgaa ctgatcaagc agtcgggtat gtaccgccag aaggcagagc ggatagtgaa
     5881 ctgcgccgaa atactgctgg aaaggttcgg cggccgtgtt cccgacacca tcgaagaact
     5941 gacctcgata cccggcgtag gcagaaaaac ggcgaacata gttctgaaca tatccttcgg
     6001 gaaagaggcc ctcgccgtcg atacgcacgt tcatagaata tccaacaggc ttggatgggt
     6061 caagaccaaa agccccgaag agactgagtt tgcgctcatg aagatcatct cgccgcatct
     6121 ctgggggccg gtcaacggtt cgatggtgga gttcggccgc cagatatgca gacccgtagg
     6181 tcccaggtgc gaggattgcg cgatcagccg ctgctgcgat tactacggcc ggatctcgaa
     6241 agaagatatc ccgcaatagc taccgccgag atgaccatca gaccgagcat cgagctgagg
     6301 aacctggcct ccagaaggta gcggctcccg ttcaatctgt atatggcgac cgcaaaagtg
     6361 gggaagtcct gtgcaccgag aatcaaagag ccgccgaact cgcccatcga aatcgcgaag
     6421 actacggttc ccgcgtagaa gagagtgttc ttgaaaaacg ggagaatcat atatctgagt
     6481 tttgccgccc tcgacgcccc gtcgagggtg gccgcttcca gaaagtccag ttcggcgttc
     6541 tgcacctgac cgttgatata gttcagcgaa acgggaagcg ccaccagcga gtacagcagc
     6601 aacatcaaaa ccggaacggg gagaaagtcg atcgtcgtga ggtaggagat ggccatcatc
     6661 gaaggcgtaa ccgagagagc cagcaatgag actatcttca actgtctggc tcccccgcgg
     6721 atcttcctgg ccgtccacgc cagataaaaa gttatcgcca cggtcgtaac cgatgcgacc
     6781 gtcgagagcg agagagaatt gaagacggtc ctcgccaacg aggtccctat gagcggcaac
     6841 tcccctttga agagcccggc cacccttcca aagatgaact ctcccctcgc ccgatcgtag
     6901 aaggaactcg tgaaagcccc gagaatcggc acccactcga aaaccagaaa aagcagagag
     6961 tacagtcgcg tggccgtctt tttctcgatg tactgttcgc cctgctcgaa ggggattctt
     7021 ctttcggaaa tggagatcag cacgccgaag agcataatga acgcgaactg gatcaccgac
     7081 agcgccacgg ccatctgggg ccgcccaagg aggcggaagt acatatatat ggagacctcg
     7141 agcgtggcga actgggcccc cccgaggatc aaaaccacgg cgaaggaagt gaagcagtac
     7201 gaaaaaatca ggagcgccga gttgaccagc gccggcacgc tcaggggaag ttcgaccttc
     7261 agaaagcgcg tcaattttcc cgcgccgtct attctggccg tctccagaag atcggaaggg
     7321 atcctcttca aagcgtttcc cagaatcagt atcactatgg gggcgttgta aaagacgtgg
     7381 cccagcagta tcgccccgaa cgagtagagt atctgaagcc tctcaaagcc gaaaagggcc
     7441 agcgcggagt ttaaaagccc gttcctgccg tagatcgcca gaaagcctat agccatggag
     7501 atccccggaa agaagaacgg tatataagtc gtcagcccca gaagctttga gagacgggat
     7561 ctgcttctgg aaaagtatat ggccgccggg agtcctgcca taagcgccag agaggtgctc
     7621 aacgccgcct gtctcagggt gaagagagtt atgccggcaa aggaaggcga ggagacggcc
     7681 tcgcccagcg agtccagacc ggaaaatccg aaggaaacta ccagcggaag gaggatcgca
     7741 gccgctacca gcaaagagag aactgtcggt acgctacttt tcgtagatta ttctctccca
     7801 ttttccgatc aattcctcga gttctacctc ggggggcagc ttcaggatcg tcgagggctt
     7861 tacggcgtac tggaagacgg ccggcatctc cacctcgatc accgggaaca tccactggtt
     7921 gagcggaaca tgcctctgaa aatccggact caacaggaaa tccaggaaca tcccggcttc
     7981 ctccgggtta tccgtccagc gtacgattcc cgcgccttct atctgcacgt agccctttcc
     8041 ctgtggaatc agaaccgaat agagagattg accgtagtaa tgcgccgagt acgcgccgtc
     8101 ggtggcgtaa gagaccatta taggcgcctc gccggcctcg aacttttcga aggagtcgtc
     8161 ccatcccagc gtgatggtca gaatcgaggg tttgagcgcg cgccagaact gctcgaaatc
     8221 ctccccgaag agcaagtatg tccatgacag gaaggccagt cccgtgctcg aggttcttgg
     8281 gtcctggagt atcagggcct tcttgaactt cccatccgtc agatcctgga gactctcgat
     8341 ctttgaggtt atcgactggc tgtcgtagat cagcgcgatc gctccgtaat cgtagggggt
     8401 cagtctccac aggggatcga agatcagatc ctggctgatc agattttcac tgccggccgg
     8461 tttgtagggc agaaatatgt cggcttcgaa ggcctttctt gaaagaaggt tgtcgattcc
     8521 gatcacgaca tcgccgtcga acttctctcc catcgagact atgtagttta cgagagagcc
     8581 ggaatcgctc agcgtcttga gcttcacttc gatccctgtt ttcagcgtga actcttcgaa
     8641 ggtttcgaga gccaggggaa cgaaggaatc gtatgaatac acgaccagca tggaaagacc
     8701 ggtcgatgcg agcaatacaa gcagcagaag agaaagagct ttcaccggaa acacctccat
     8761 tttctgaaaa atagccggag gcgtctcccg aagccttccc tacgccggta ctaaccggat
     8821 caggttcctg gggtctgcac ttaagcactc tcagcctctt gggctccccc ggcacccaat
     8881 tataacaccc gcgagttggt gatacaattg ctctggaggt ggtcatttga atcatctgaa
     8941 ggctgtaatc cttatcaccg gtgatgagat aacgaagggg attgtcaagg atacgagcgg
     9001 gggttacctg gccgagagtc tgaccgccat gggctacgaa gtccagtcga tcgttacggt
     9061 acaggatgat gctcaatgta tgatacggga gatgaaaggc gcgctggaaa gggccgatct
     9121 tctcgtaata accggaggtc tggggagcac tttcgacgac ataaccctgc aaacggtggc
     9181 ccgatacgcc gggcggccgc tgatctacga tggcgattat cacgggagtc tcgagaggag
     9241 cttcgaatcg cgcttcagga gaaaagctcc cgccgatctc gaacgccagg cctataccgt
     9301 agagggctcg actatcctgc ccaacgccgc cggaagcgcc cggggatgct ttctgaagat
     9361 gggcgccgga gagatagtag tcctgccagg tcccccgatg gagatgaaag ccgtcttcgc
     9421 cgaggcgatc aaacacatga gccgtccggc ggcgcccttt actcggaccg tcgggcttgt
     9481 agatataacc gaacccgaga tagaaaaaac tctggaggaa ttgctggata gattcgtgaa
     9541 tgtgaaattc gtcacccgtg tcaaatacta caccgggccg acggttatac tctccggccc
     9601 gaacggcgag cagctcgaag agatcatcgt cctgctcaga gagaagtatg gtgagaacat
     9661 ctatacgacc atcggcgagg aactcgctga cgttgtggtg aagatgctcg tcgaaaggaa
     9721 aaagacggtt tcgacggccg agtcctgcag cgggggaagg ctttcggcca tcctgacctc
     9781 cgttccgggg gtgtcggcca tcttccccgg tggtatagtc gcctattcga acgaggtgaa
     9841 aacctctctg ctgtcggtga gaagagagac gctggagagt ttcggagccg tttctgaaga
     9901 gacggccggg gagatggccc gcggcgtcgc cgccgttttc ggaacggaca taggcctttc
     9961 tgtgagtgga atcgccggtc cgtcgggcgg gtcgatcgaa aaaccggtcg gactcgtctg
    10021 cacggccata agtttcggaa agaggtcctg gagctacacg gaccgtttca gaggcgatcg
    10081 tgaaaccatt cagctgagag cggcccacac cattttcaag agactctgga ggatactttg
    10141 gaaagaagat tatacagggt aaacgacccc gtttcatgga tagagaaaaa cctctcctcg
    10201 ttcgacaggg tgctcgtaat ctttcccacc gagagaatgc tcgaatcttt tgcggccgtt
    10261 cacgccagaa aagaggagga gggttacttc ttttctcacg atgtctttcc cttcgaggag
    10321 atcggcacct cgccgcgcat acgcgccgaa agggtctcgc tgctctacaa tctctcgctg
    10381 ggcaggttga agaaagtctt cggctcattc cacggccttc tcagaaagac cgttccgctc
    10441 gacgttttcg gcgaactgac gaccgctata cgcacgggag gagaactaga ggtcgacgaa
    10501 gagaacctgg tcagaatggg ctattgtaga acgttcaacg tcaccgttcc cggcgaattt
    10561 tcgatcaagg gctcgataac ggatatcttc gttccgggca acggccatcc gctcagaata
    10621 gagaccttcg acagggagat cgagtctata aggttcttcg atccttcaac gcagaagagc
    10681 gt
//
LOCUS       DAOOLY010000061        10626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7070_length_10626_cov_3.395989, whole genome shotgun sequence.
ACCESSION   DAOOLY010000061 DAOOLY010000000
VERSION     DAOOLY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7070_length_10626_cov_3.395989"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLD15_04590"
     CDS             complement(<1..358)
                     /locus_tag="PLD15_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB63213.1"
                     /translation="MIDFQKLADDYGTPLYVYDAVEIRERIHRAREVFEGLNAGIVFA
                     LKANGNPALLKIMAQEGVGADVVSKGELLAAKMAGMKTIVWNGNGKCASERDHFTNKG
                     INYVCIDSFEELPLWDG"
     gene            complement(360..1457)
                     /locus_tag="PLD15_04595"
     CDS             complement(360..1457)
                     /locus_tag="PLD15_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB63214.1"
                     /translation="MLSRVYDHLSLKVERAHGVRISTDRGEFLDTFAGIGVLAFGHTY
                     EETIEAIKEKIGRFSHTSNYFLDEDAISLGQMLLRMSGGEGEVFFTNSGTEATEAAIK
                     AIRKLRRGKLVSFEGNFHGRTMGALSLTHSPKLRTPFEPLLPDTVFLPLSGKTFEEFV
                     GSNEIAGVFVEPVQGNSGVHPLPEELIEVISSMKEKYGFLIVADEIQSGLCRTGDFFA
                     YQHHDLRVDIITVGKAVGGGLPLGAAIFCGVRPFAIGDHGSTFAPNPVSLAAGKSVVE
                     RMDTTLLEDVKIKGEYLRHGMERLEWVKEVRGRGLMLGLSTLDPIRIKEKAFDRGVLL
                     NVTGGSIRLLPALNISIDEIDEILERLDFGG"
     gene            complement(1470..2672)
                     /locus_tag="PLD15_04600"
     CDS             complement(1470..2672)
                     /locus_tag="PLD15_04600"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPB63215.1"
                     /translation="MIVVQKYGGSSVADIERIKNVAARIKKRVETGDKLIVVVSAMGK
                     TTNELIALAGNVSSQPSPRELDMLLATGEQVSAALLSIALMELGVRSISYNAFQLNIN
                     TFGEFSNARIADINLNKIYSELEARDVLVVTGFQGINPEGDLTTLGRGGSDTSAVAIA
                     AKAGVPCEIYSDVAGIYTCDPKVHEKARKLEYITYDEMLELAALGAKVLHSRSVEIAK
                     KYNVELICLSSFSEEGGTRVVAKLPEWLEQPVVTGATIDTNQLKVTINYLPGDTDVLS
                     RIFNAVAKASFNVDMISVVNENGHSHLTFTVLSANPAAIRRTIEGVLKDVDGWELTLD
                     DNVAKVSTVGVGMRSSAGVAARFFSSLQKTGVRIIATTTSEIKISVLVPRTDASKALR
                     VLLDEFDL"
     gene            complement(2669..3373)
                     /gene="dapD"
                     /locus_tag="PLD15_04605"
     CDS             complement(2669..3373)
                     /gene="dapD"
                     /locus_tag="PLD15_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HPB63216.1"
                     /translation="MDAETIIKLISQSKKKTPALCYLSGKLHGIESSDLTYIGGEDFG
                     IIIGDLKEIKAFLERNKEVILNHYIEVRARNSALPLADLTKYEARIEPGAIIRDLVEI
                     GKNAIIMMGAVLNVGSVIGEGTMIDMNVVVGGRALVGRNCHIGAGAVLAGVIEPPSAK
                     PVVIGDGVLVGANAVVLEGVTVGEHSVVAAGAVVTKDVPPFSVAAGIPARVIKQFDEK
                     TASKTQLVKELREIQQ"
     gene            complement(3355..3972)
                     /locus_tag="PLD15_04610"
     CDS             complement(3355..3972)
                     /locus_tag="PLD15_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB63217.1"
                     /translation="MGHEIKEVFSAHELVLEVDESGIFRRGEPDVVVDFSNRSVLATT
                     LQLCSQYSAGLVIGTTALVEEDMQAIKELSNRSPVVQSYNFSTGINILKILLRESVSL
                     FEGMDVEIVETHHNKKKDAPSGTAILLKEALGREVPVHSLRIGGVPGDHTITFANDGE
                     VLQISHRAISRRVFAIGALRAAEFCMSVKSGFYRFEEVLRWTQKR"
     gene            complement(4002..4883)
                     /gene="dapA"
                     /locus_tag="PLD15_04615"
     CDS             complement(4002..4883)
                     /gene="dapA"
                     /locus_tag="PLD15_04615"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPB63218.1"
                     /translation="MFRGTGTALVTPFKNGKVDYDSFRRFLRFQLEGGVEALVVLGTT
                     GESPCVKDSERKELIRIAIEESGGKVPVIVGTGTNSTQKTRELSSRVFDDGADGVLVV
                     TPYYNKPTQEGLYRHYGELATRVGGPIIIYNVPGRTGVNMLPETVLRCAEFDNIVGIK
                     EASGNQYQVDYLISTVRSRGLSLKIWSGNDDQAFHLCCSGGDGVISVLSNVAPADTVT
                     MIKAVLAGDLEKARELHLRLLPLMNALFIETNPIPVKYAVHLLGYCENELRLPLVELS
                     DKGVKAVEEAMKTSGVL"
     gene            complement(4853..5569)
                     /locus_tag="PLD15_04620"
     CDS             complement(4853..5569)
                     /locus_tag="PLD15_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPB63219.1"
                     /translation="MKGINYSATGNTFLVVDSREERLDDETKHNIVVKNVDKNDGVIF
                     VEDGFMDFYNRDGLRASFCGNGARTYVYFIHEKEEREKVSFKSMAGEIEGVVSRGVVS
                     IRMPDPILTGSFEEEGFSGEIITVGVPHIVMEGDTEKIDWELLVPLRHLYDANVNVYS
                     VIEKGKLRIRTYERGVEAETGACGSGATSVSWIYAKKTGLKSVELKANGGWLTVTFED
                     GKAYLGGGVEKCSGELELLW"
     gene            complement(5571..6536)
                     /locus_tag="PLD15_04625"
     CDS             complement(5571..6536)
                     /locus_tag="PLD15_04625"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPB63220.1"
                     /translation="MKLAVVGATGEVGRMMLKKLEEEAITPEEIDLFSSSRSAGSLMK
                     FAGKSIEVRELREDSLRRDYDYVLFSAGASVSKTFAPLAASCGAVVIDNSSAFRQSEE
                     IPLVVPEINGCLLKGYRGIVANPNCSTIQMVLSLHKIHRRFGLKTIVVSTYQAVSGAG
                     NRGIVELERQEAGSQDQAVFVRKIHRNVVPVIGDILENSFSAEEMKMINETRKILGDS
                     KVSIWPTTVRVPVAHGHSEAVFCETEKPFELEELKEELRASEDVVLTDERFTPVEIAG
                     SDLVYVSRVRSFDSHRFLLWNVADNIRVGAATNAVRILKMHAGGA"
     regulatory      complement(6613..6781)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(6959..7408)
                     /locus_tag="PLD15_04630"
     CDS             complement(6959..7408)
                     /locus_tag="PLD15_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63221.1"
                     /translation="MILDDVERRLLNQIYARVFGREKRYLPVDVLAGESGICPEEFSR
                     VLAFLDEEDYVEVKPFRMLRITHKGILACEEGKIPEKREIRDRVMEKIKELSQGDTEV
                     FIVIDALAGEMNLMRYELFDILNYLQGEGLVKIHSRISVSLCEKTPE"
     gene            complement(7405..8175)
                     /locus_tag="PLD15_04635"
     CDS             complement(7405..8175)
                     /locus_tag="PLD15_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPB63222.1"
                     /translation="MFTNDQGRYFISNKIWDEEFSDSWISDQKMGKSQPPLEKPFVEE
                     GLLPLPDPLTVTLKKKDITSIIHDRKSRRVFTDEEITSGELSYLLWASQGIKEIMANN
                     YATLRTVPSAGARHPFETYLYINRVSGIEKGIYRYVASRHSLSLLKAGNFSDEMTEAN
                     LGQQFVGNSAVTFLWSVIPYRTEWRYNLHSYKVILMDAGHVCQNLYIACEALRLGTCA
                     IGAYSHEKINRLLNLEGEDEFVVYLAPVGRYRERRNPK"
     gene            complement(8578..9237)
                     /locus_tag="PLD15_04640"
     CDS             complement(8578..9237)
                     /locus_tag="PLD15_04640"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPB63223.1"
                     /translation="MVVFIRRNRVELLIRGSIVSWGWVIALKDRIGAGIYIYPMPLVV
                     VGSMAEDRPNYTTVAFCGVVEVQPPMIQITLAKTRFSCKNIREFESFSVNVPSMDMLR
                     GVDFMGIYSGKTTDKSELFKTFYGELQNAPMIEEAPLNMECRLVDVFDFGGTHETFIG
                     EVVQSYCSEFCVVNGLPDIKKINPATFSVYDNSYWSLGIHLGKAWNIGMKYTPPKTIH
                     D"
     gene            9320..10441
                     /locus_tag="PLD15_04645"
     CDS             9320..10441
                     /locus_tag="PLD15_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPB63224.1"
                     /translation="MSSVYEDVVWVDFDLLERFMVDVFKSVGVPAEDAKICANVLITS
                     DKRGIDSHGIGRLKPIYIDRIREGTQKPVTEFEIVKESPTTAVVDGHDGMGHVIAYKS
                     MKIAIEKARKFGMGMVAVRNSTHYGIAGYYPLMAIEAGMIGITGTNARPSIAPTFGVE
                     NMLGTNPLTFGMPTDEEFPFVLDCATSVAQRGKIEVYGRASKEIPEGWVIGPDGKTRT
                     DTQQILIDLNTGGAALVPLGGIGEETAGYKGYGYATVVEILSAALQGGSFLKMLTGIG
                     ENGEKKPIRIGHFFMAINVEAFTSLEEFKKTTGDILRELRASRKASGEERIYTAGEKE
                     YLAWLERKDRGAPLNDVLRKQLVQLRNEFKLGNYKFIWE"
BASE COUNT         2589 a         2798 c         2391 g         2848 t
ORIGIN      
        1 ctccatccca cagtggaagc tcttcaaagg aatcgataca tacatagttt attcccttgt
       61 ttgtgaagtg atccctctcg gaggcacatt ttccgttacc gttccagacg atcgttttca
      121 ttccggccat tttggccgcc agaagttcgc ctttggaaac gacgtcagct ccaacacctt
      181 cctgtgccat tatcttcagc agggcaggat ttccattggc cttcagggca aaaactatac
      241 cggcgttgag cccttcgaag acctcccggg ctctgtgtat tctctccctt atctccacag
      301 cgtcgtacac atagagcggt gtgccataat cgtccgccag tttctgaaaa tcgatcatct
      361 caacctccaa aatctaatct ttcaagtatt tcatctatct catcaataga gatgttcagt
      421 gcaggaagca accttatgct ccccccggtg acgttgagca ggacacctct atcgaaggct
      481 ttctccttta tccggatggg atcgagagtg gaaagcccca gcatcagacc ccttcctctg
      541 acttccttta cccattcaag tctttccatt ccatggcgta agtactcacc ttttatttta
      601 acatcttcga gtagagtggt atccattctc tccacaacgg atttcccggc ggccaggctc
      661 acgggatttg gcgcaaaggt tgaaccatgg tctcctatag cgaagggtct cacaccacaa
      721 aagatagcag cgccgagagg aagtccgccg ccgacggcct tacccacggt aattatatcg
      781 actcgcagat cgtgatgttg gtaggcgaaa aagtccccgg tcctgcaaag accgctctgt
      841 atctcgtcgg cgactatcag aaaaccatac ttttctttca tgctggatat aacttcgata
      901 agttcttcgg gcaaaggatg aactccgctg tttccctgta caggttcaac gaaaacgccc
      961 gcaatttcgt tgcttccgac gaactcctca aaagtctttc cagaaagcgg aagaaaaacg
     1021 gtgtctggaa gcaaaggctc gaaaggtgtt ctaagtttcg ggctgtgggt aagcgagagt
     1081 gctcccatcg tcctaccgtg aaagttgccc tcaaaggaga cgagttttcc ccttctcaat
     1141 tttctgatcg ctttgatcgc cgcctccgtc gcctcagtac ccgagttggt gaagaagacc
     1201 tcgccttccc ccccgctcat gcggagcaac atttggccga gtgatatggc atcttcatcg
     1261 agaaaatagt tggaggtatg agagaatctt ccgatctttt cttttatggc ttcaattgtt
     1321 tcctcatagg tatggccgaa ggcgagaaca cctataccgg cgaaggtgtc aagaaactcg
     1381 cctctatcag tcgatattct tacgccgtga gccctttcca ctttcagtga aagatgatcg
     1441 tacactctcg aaagcattca attcaccctc tatagatcga actcgtcgag caacactctt
     1501 agagctttgc tcgcatcggt ccggggaacc agcacagata tctttatctc cgaagtcgtt
     1561 gtggcgatta ttctaacacc ggtcttctgg agcgaggaga aaaacctggc agcaaccccc
     1621 gcactcgatc tcataccgac tcccactgta gatacttttg ccacgttgtc gtcgagtgtg
     1681 agttcccatc cgtctacatc tttcaagacg ccctcaatgg tccttcggat agctgccggg
     1741 ttggccgata gaacggtgaa ggtaagatga gagtggccat tctcattgac taccgagatc
     1801 atatcgacgt tgaaactggc ctttgcgacg gcgttgaata ttctgctgag tacatctgtg
     1861 tcgccgggga gataattgat ggttacttta agctgattgg tatctattgt agcgcctgtt
     1921 acaactggct gttcgagcca ttctggcaat tttgccacca ctctggtacc tccttcttca
     1981 ctgaaagatg agagacagat cagctccacg ttgtatttct tggctatttc cacagaacgc
     2041 gaatgaagta ccttcgcacc cagagccgcg agttcgagca tctcatcgta tgttatatat
     2101 tccagttttc ttgctttctc atgcacttta ggatcgcacg tatatattcc ggccacatcg
     2161 ctgtatatct cacatggcac accggctttt gccgcaatcg ccacggcaga ggtatcggac
     2221 ccacctcttc caagcgttgt aagatcgccc tccggattta taccctgaaa gcccgtaact
     2281 acgagaacgt ccctggcttc gagttcagaa tagattttgt tgaggtttat atcggcgatc
     2341 ctggcgttac tgaactcgcc gaaggtgttt atgtttagct gaaaagcgtt ataagagatg
     2401 gatctcacac cgagttccat cagggcgata ctcaggaggg ctgctgaaac ttgctcgccg
     2461 gttgccagaa gcatatcgag ctcgcgtggg ctgggctggg acgatacgtt accagcgaga
     2521 gcgatcaact cgttggtagt tttacccatg gctgaaacta ccacgatcag tttatcacca
     2581 gtctccaccc tcttctttat tctggcagcg acatttttta ttctctctat gtcggccaca
     2641 gatgaacctc cgtacttttg gactactatc attgttgtat ctccctcagt tccttaacga
     2701 gttgtgtctt gctcgccgtt ttctcatcga actgctttat aacccgtgcg gggatgccag
     2761 cggcaaccga aaagggtgga acatccttgg tgactaccgc gcccgcagcc acgaccgagt
     2821 gttcacccac cgtcacacct tctagaacta cggcgttggc acctaccaga acaccatcgc
     2881 ctatcactac cggcttcgcg ctgggaggtt cgattactcc tgcaagaacc gctcctgcgc
     2941 cgatatgaca gttcctaccg accagggcac gtcccccaac cacgacgttc atatctatca
     3001 tcgtgccttc tcctatcact gaaccgacat ttaacaccgc ccccatcatg atgatcgcat
     3061 tcttgccgat ctcgaccaga tcgcgaatta tggcaccggg ctctatcctg gcctcgtatt
     3121 tcgtgagatc ggcaagagga agtgcggagt tccttgccct cacctctata tagtgattta
     3181 aaatgacttc tttatttctc tcgagaaagg cttttatctc tttcaggtct ccgatgatta
     3241 tcccgaagtc ctctccaccg atgtatgtca aatcactaga ttcgattcca tggagcttac
     3301 cgctgaggta acacaacgcc ggcgtcttct tcttcgactg acttatgagc tttattatcg
     3361 tttctgcgtc catctcaaaa cctcctcgaa tctatagaag ccgcttttca cactcataca
     3421 aaactcagcc gctcttaggg caccgattgc gaaaaccctt ctcgaaatcg cacgatggga
     3481 tatctgtaga acctctccgt cgttggcgaa cgttatcgta tgatcgccag gtacgccgcc
     3541 gatgcgtaga gagtgtaccg gcacttctct tccgagagct tccttgagca gtatggccgt
     3601 gccggaggga gcatccttct ttttgttatg atgggtctca acgatctcca catccattcc
     3661 ctcgaagagg gagaccgatt ctctgagcag tatttttaat atgttgattc cggtcgagaa
     3721 attgtagctc tgaacgaccg gtgaacggtt tgataactct tttatagcct gcatgtcttc
     3781 ttccacaaga gctgtggtac ctatcaccaa gccagcactg tattgagagc atagctggag
     3841 agtagttgca agaaccgacc gatttgagaa atctacgaca acgtccggtt ctccacgtct
     3901 gaaaataccg ctttcatcta cttcgagaac cagttcgtgg gccgaaaaga cttctttaat
     3961 ttcgtgtccc attctcccgg aggatcccac cagcccgtac ctcataacac tccactcgtc
     4021 ttcatcgcct cttcaactgc ctttacacct ttatcgctca attccaccag aggcaacctc
     4081 aactcgtttt cacagtatcc cagaagatgc acggcgtatt tgacgggtat aggattcgtt
     4141 tcgatgaaga gtgcattcat aagtggcaag agcctcagat gaagttcacg cgccttttct
     4201 agatcgccgg ccaaaacagc ctttatcatg gttacggtat cggctggggc aacgttggag
     4261 agtaccgata tgactccgtc tccaccactg cagcagagat ggaaggcttg atcgtcgtta
     4321 cctgaccaga ttttgagaga cagccctctg gacctgacgg tcgagatgag ataatcgacc
     4381 tgatactggt ttccgcttgc ctcctttata ccgactatgt tgtcgaactc cgcgcatctg
     4441 aggaccgtct cgggaagcat attgaccccc gttcttccgg gaacgttgta aattatgatc
     4501 ggaccaccca cacgtgtcgc cagctcgccg taatgtctat aaaggccttc ctgagttggt
     4561 ttgttataat atggcgttac caccagtacg ccatcggctc cgtcgtcgaa gaccctggaa
     4621 ctcagctccc ttgtcttttg tgtagaattg gtccccgttc cgactatcac gggtactttc
     4681 ccgcctgatt cttctatagc gatcctgata agctcttttc gctcgctgtc ctttacacag
     4741 ggcgactcgc cggttgtgcc tagaactaca agggcttcaa ccccaccctc gagctgaaat
     4801 ctcaaaaacc tcctgaagga atcgtaatcg acctttccgt tcttgaacgg tgttaccaga
     4861 gcagttccag ttcccctgaa catttctcca cacctcctcc tagataggcc tttccatctt
     4921 cgaaagtgac tgtaagccag ccgccgttgg ccttcaattc cacgcttttt agtcctgtct
     4981 ttttcgcata tatccagctc acagaagtgg cgccacttcc acatgcaccg gtttcggcct
     5041 ctactccgcg ttcgtaggtc cttatccgca actttccttt ttcaatgacc gagtaaacgt
     5101 tcacgttggc atcgtagaga tgcctgagcg gtacaaggag ctcccagtcg attttctctg
     5161 tgtctccctc cataactatg tggggtactc caacggttat gatctctccg gaaaaccctt
     5221 cttcttcaaa actgccagtt agaatcgggt ccggcattct gatgctcaca actccccgcg
     5281 agacgactcc ttcgatctcg ccggccatac ttttgaaact aaccttctct ctttcctctt
     5341 tttcatgtat gaaatatacg taagtccttg cgccgttgcc gcaaaagctg gctctcagtc
     5401 catcacggtt gtagaagtcc atgaagccgt cttcgacaaa gatgacaccg tcatttttgt
     5461 cgacattttt gactacgatg ttgtgtttgg tctcatcgtc aagtctctcc tctctggaat
     5521 cgacaaccag aaaggtatta ccagtggccg aataattgat tcccttcatt ctaagcgccc
     5581 cccgcatgca tcttcaatat tctcacggcg tttgtggcag ctcccaccct gatgttgtcg
     5641 gccacgttcc acaagagaaa acgatgactg tcaaaggatc tgactcttga aacataaaca
     5701 agatccgaac cggctatctc gaccggtgtg aacctttcgt cggtcaaaac aacatcttcg
     5761 ctcgcccgaa gttcttcctt gagttcctca agctcaaagg gtttctcagt ctcgcagaaa
     5821 accgcttcgg aatggccatg cgcgaccgga actctgacgg tggtaggcca gatggatact
     5881 ttagagtcgc ccagtatctt tctggtctcg tttatcatct tcatctcctc agccgaaaag
     5941 gagttttcaa gtatatcgcc gataacagga accacgttcc tgtggatttt tcttacaaat
     6001 accgcttgat cctgtgatcc ggcctcctgc ctctccagct cgacaattcc cctgttcccc
     6061 gcacccgata cggcctggta ggtggatacg acaatcgttt ttaggccgaa cctcctgtgt
     6121 attttatgca gtgacagcac catctgtatg gtcgagcagt tgggattggc cacaatgcct
     6181 ctatagcctt tcaaaagaca gccgttgatt tccggaacca caaggggaat ctcttcactc
     6241 tgtctgaaag ctgaagaatt gtctatcaca accgctccgc aggaggctgc aagcggagca
     6301 aaggtttttg aaacggaggc cccggccgag aagagaacat aatcgtaatc cctccggaga
     6361 gaatcctctc tgagttctct cacttctata ctctttccgg cgaatttcat aagacttccc
     6421 gcgctccggc tcgaggagaa aaggtctatt tcctcgggtg taatcgcttc ttcctcgagc
     6481 tttttaagca tcattcggcc aacttcaccg gtggctccaa caactgcaag tttcataaca
     6541 tcacctccaa aataaaaagt gttcccgaat gagaccggga acacctaatg gtttgaaata
     6601 atcgcttatt ccaaacccac cggctctcca cggactcctc cgtgacagtc atacggatgt
     6661 tctccgtatg cccaggatga cgggatctgg gtctcccgtc tccttcggca ccctagcctt
     6721 tcgatccgcc cccattccct gatgggcgtc tactcttcac gggtgctcct ccgttgggtt
     6781 actttcgctt gattatagca cgcgttttcc tgactgtaat ttgacaatga aaatatacag
     6841 taaattgttc attttcttta tatcctcaat aaccatcgtt tatgtttatg tagcgatagt
     6901 ggcagtttat ggcttgttta tagcgagcgg gactaaagct ccataaacga acaaacggtc
     6961 attccggtgt cttttcgcag agagagacgg aaatcctcga atgaatcttc accagcccct
     7021 cgccctgaag gtaattcaga atgtcgaaca actcgtatct cattaaattc atttcaccgg
     7081 caagtgcgtc tatgacgata aaaacctccg tatctccctg tgagagctct ttgattttct
     7141 ccataactct atccctgatc tctctctttt cggggatctt accctcttca caagccagta
     7201 ttcccttgtg ggttattctg agcattctga agggttttac ctctacgtag tcctcttcat
     7261 ctagaaaggc taaaaccctg ctgaattctt cggggcatat gccgctttcg ccggccagga
     7321 catctacggg caaatacctc ttttcccgcc cgaaaactct cgcatatatc tgattaagca
     7381 atcttctttc cacgtcatcc aagatcactt cggattcctc ctttccctat agcggccgac
     7441 aggagccaaa tagaccacga attcgtcctc tccctcaagg ttcaaaagcc tgtttatctt
     7501 ctcatggctg taagcgccga ttgcacacgt accaagtcta agagcctcac aggcgatgta
     7561 aagattctgg cacacgtgac ccgcatccat aaggatcact ttgtaggagt ggaggttgta
     7621 tctccactca gttctgtacg gaatcaccga ccagaggaac gtcacggcag agttgccaac
     7681 gaattgctgt ccgagattgg cctcggtcat ttcatcggag aaattcccag ctttcaagag
     7741 gctgagcgag tgtctcgatg ccacatagcg gtagattcct ttttctattc ccgaaactct
     7801 gttgatatac agataagtct cgaaagggtg ccgtgcaccg gccgaaggta cagttctcag
     7861 agtcgcgtaa ttgttagcca taatctcctt tattccctga ctggcccaca aaaggtacga
     7921 aagctctccg gaagttatct cttcatccgt gaagacccta cggctttttc tatcgtgtat
     7981 tatcgatgtt atgtcttttt tcttgagagt aaccgtaagc gggtctggaa ggggtaacag
     8041 accttcttca acgaaaggtt tttctagagg cggttgagat tttcccatct tctggtctga
     8101 tatccaggaa tcgctaaatt cctcatccca tatcttgttg cttatgaagt atcttccctg
     8161 atcgttcgtg aacattcaaa ccctcccttg ccatcgctga taactaaagt ctagcatgcg
     8221 attcttgtgg aaagtacgta agattgagcg atctttataa gagacattgc acccgaactg
     8281 atggttagcc ggtacactta cagaccgata agaggaaaag aaaagtaacg gtgggtttct
     8341 tttacaacat tttctgcttc acagtgatat ccaggatcaa cgggtgaaat agattgtatc
     8401 ggggtttgaa gatagaaccg tcctcagatc ccacgagata gatgaaccag gtgcgaagga
     8461 aagttccagg cttgcaagga tcatttcgtg aactgcgaag agttttttgg gctattaggt
     8521 acccagagtc ggtacaaaag tcgcaatcgt cgaggtgccg atgacgctgc tatccgatca
     8581 gtcgtggatc gtttttgggg gtgtgtattt catcccgatg ttccaggctt ttccaaggtg
     8641 aattcccagt gaccagtaag aattatcgta caccgagaag gtcgcgggat ttatcttctt
     8701 tatgtcgggt aatccattga ctacgcagaa ctccgagcaa tagctctgca caacttcccc
     8761 gatgaaagtc tcatgagtac ctccgaaatc gaagacatcg acaagcctgc actccatgtt
     8821 caaaggcgcc tcttcgatca tgggagcgtt ctgaagctcc ccgtaaaagg ttttgaaaag
     8881 ttccgactta tcggttgtct tacccgaata gatgcccatg aagtcaaccc ctctgagcat
     8941 atccatggaa ggaacgttta cactgaacga ttcgaattcc cttatatttt tacagctgaa
     9001 acgtgtcttc gcaagagtaa tctggatcat tggaggctga acttcaacaa ctccacagaa
     9061 agccaccgtc gtatagttag gcctgtcttc cgccatactt cccactacta ccaacggcat
     9121 tggatatata tagattccag cacctattct gtccttcaaa gcaatcaccc atccccatga
     9181 aacgatactt ccccttataa gcaattctac acgatttctt ctaataaaga caaccatggc
     9241 cgccaaagtt ctggtcaggc ttatcggaca gatccattgt ataattccac tgtgacgaaa
     9301 cgaaagggag ggaaatgatt tgtccagtgt ttacgaagat gtggtgtggg ttgacttcga
     9361 tctgctggaa cgcttcatgg tagatgtctt caaaagtgtt ggtgttcccg cggaagatgc
     9421 gaagatctgt gctaacgttt taataacttc cgacaaaaga gggatagact ctcatggaat
     9481 cggaagacta aaaccgatct acatagacag aattcgcgaa ggtacacaga aaccggtgac
     9541 ggaatttgag atagtcaaag aaagtccaac aacagccgtt gtggacggtc atgatggtat
     9601 gggccatgtg atcgcgtata aatcaatgaa aatagccata gaaaaagcca ggaaattcgg
     9661 tatggggatg gtggcagtca gaaattcgac acactatggt atagccggtt actacccact
     9721 catggcgatc gaagcgggta tgatcggtat taccggaacg aacgcccggc cctcgatcgc
     9781 cccgacgttc ggagtggaga atatgctcgg taccaatccg ctaacgtttg gaatgccaac
     9841 cgatgaagag ttccccttcg tacttgactg tgccacatcg gtcgcccaga ggggaaagat
     9901 agaggtttac gggagggcca gcaaggagat ccctgaaggc tgggtgatag gtccggatgg
     9961 aaagacgaga acggataccc aacagatact gatcgatctc aacacgggcg gagcggctct
    10021 ggtccctctg ggaggcatag gcgaagagac ggccgggtac aaaggctatg gctacgccac
    10081 tgtggtcgag atcctgtcgg cggcccttca gggagggagt ttcctcaaaa tgctgaccgg
    10141 tataggcgag aatggcgaaa agaaaccgat aaggataggt cacttcttca tggctataaa
    10201 tgtggaagcc tttacatcgc tcgaagaatt caaaaagacc acgggcgaca tactgagaga
    10261 attgagagcc tccaggaagg catctggaga ggagagaata tacacggccg gtgagaagga
    10321 gtatcttgcc tggctcgaaa gaaaggaccg cggagctcct ctcaacgatg ttctgaggaa
    10381 gcagctggtc cagctcagaa acgaatttaa gctgggcaat tacaagttta tctgggaata
    10441 gaaatggagg tgatggagtc tctcaaaggg actcttatat atgaagatcg gagttgttgg
    10501 tttcggtgcc gctgcaattg gcttcgtaag tgagataatg aacaggaatc atgaaataca
    10561 tatcctggag agatcgaaag acatttacag ttccagcata agtgggattc gctcggatgg
    10621 aaagat
//
LOCUS       DAOOLY010000062        10505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7183_length_10505_cov_6.145263, whole genome shotgun sequence.
ACCESSION   DAOOLY010000062 DAOOLY010000000
VERSION     DAOOLY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7183_length_10505_cov_6.145263"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2912)
                     /gene="nifJ"
                     /locus_tag="PLD15_04650"
     CDS             complement(<1..2912)
                     /gene="nifJ"
                     /locus_tag="PLD15_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPB63225.1"
                     /translation="MARVMKTMDGNTAAAHVAYAFTEVAAIYPITPSSTMAELADAWA
                     ANGLKNIFGQVVDVIEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMVPNMYKIAG
                     ELLPGVFHVSARAIAGHALSIFGDHSDVMAVRQTGFALLASGSVQEIMDLGSIAHLSA
                     INSRVPFLHFFDGFRTSSEVQKIEVLDYEELAKLVDYEAVKRFKDDALNPEHPKTMGT
                     AQNPDIFFQAKEASNRFYDAVPDIVAKYMDEISRLTGREYKPFVYYGDREAENIIIAM
                     GSVTETVEETVDYLLKKGEKVGVLKVHLYRPFSAKHFFDVLPKTVKKIAVLDRTKEPG
                     SLGEPLYEDVKTLFYNCEDAPLVVGGRYGLGSKDTTPSQIKAVFDNLKNTAPKDHFTI
                     GIIDDVTNTSLEIKDKINTAPESTIRCKFWGFGSDGTVGANKDAIKIIGDYTKLYAQG
                     YFAYDSKKSGGVTISHLRFGKKPIKSTYLIDEADYIACHKPAYVNQYDLLDGLKKGGT
                     FVLNTGWSLEELDKHLPASMKRYLAENDIKFYTIDATKIAVEIGLGNRINMIMQSAFF
                     KLANVIPIEDAVEHLKDAIVKSYGRKGEKVVEMNYRAVEQGISALVKIEIPAAWKNAV
                     DEKIEDSDRPEFIKKVVDTMNRQEGDKLPVSAFVGRENGEFPSGTTAYEKRGIAIDIP
                     EWQLENCIQCNQCSYVCPHAAIRPFLVNAEEASKAPATFEMKKAVGGKAFEGLQYKIQ
                     VSPLDCTGCGNCADICPAPNKALIMKPLESQLEKEIPNWNFVNTISEKKDLMNVETLK
                     GSQFVKPLLEFSGACAGCGETPYAKLVTQLFGDRMLIANATGCSSIWGASAPATPYCV
                     NAQGHGPAWANSLFEDNAEYGFGMAMAIKQGRNKLASIIQELLVLDIPEDVKAPFEAW
                     LEGKEDAKASKAAALDILSVLSRDCKSEAARQLMKEIEERKDLLIKKSVWVFGGDG"
     gene            3231..3710
                     /locus_tag="PLD15_04655"
     CDS             3231..3710
                     /locus_tag="PLD15_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63226.1"
                     /translation="MTLSRKQIAIIIGAAVLFTGLFLPAISLKANNPLANIAGSSNYI
                     QADQAYGVILLLLSLIVVALAIVKKFQWAFIAAVGTLGILVFRLVTFLTDISKFNSLQ
                     SSMNPGPFSPEFDGVMGMTQEIAVGISLNWISWIVMFAGSIITIVVLLREDKIREIK"
     gene            complement(3736..4716)
                     /locus_tag="PLD15_04660"
     CDS             complement(3736..4716)
                     /locus_tag="PLD15_04660"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPB63227.1"
                     /translation="MKTAYIESNSFDPWLNLSIEEYLLDNYARDNAVLYLWQNAHTVV
                     IGRNQNAWRECRIENLERDGGKLARRLSGGGAVYHDLGNLNFTFLLPRLEYNLHRQLA
                     VIVDAVKGLGIEANFSGRNDILAEGKKFSGNAFYHGRQASYHHGTVLIDVDMSRLSMY
                     LNVSKAKMESKGVRSVASRVVNLKDLKPDLTIEIMKRAMYDGFIEEYGHIDRILDYDE
                     IASREEVKTLYSKYSSREWLLGKSPEFDFEIENRFVWGGIEIGLNVIKGMIREAVVYS
                     DSMDVDFIELLRSSLKGIEFSRESLTGTLASLPENKERADLIEWFDQVEL"
     gene            complement(4723..5562)
                     /locus_tag="PLD15_04665"
     CDS             complement(4723..5562)
                     /locus_tag="PLD15_04665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPB63228.1"
                     /translation="MRVKGLIFLVVLVFLCTALGNSYVRYLVKPGDKLFNIIRDYNIR
                     ISTVLDFNCIDDPRCLESGQAIYIPVRDGFFYDVQDGDSPEYIAKLFFALVEDIMSAN
                     RLSYSSKIYTGQRLFIPLEIVSVCSNMNISTQYSWPVYGKISSEFGWRKDPFTGASTF
                     HSGMDIAVQEGAPVFAARDGTVIEAADNGGYGLNIIILHADGSKARYAHLSHISVYSG
                     QRVARGELIGRVGETGRATGPHLHFEIVDSKNQCKDPRSYLSSSQYMYVRKESGSLGL
                     GGK"
     gene            complement(5787..6107)
                     /locus_tag="PLD15_04670"
     CDS             complement(5787..6107)
                     /locus_tag="PLD15_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63229.1"
                     /translation="MFEKIDYWVTAASYADRKGTWFLEAALIHPNIGESHEFDEEWTR
                     EQILEKCDVFVFCTIAKNEKGEWKRMEQVRKIKMEQGEFLRVDGKEKAGDQLGFLPTI
                     EEVL"
     gene            6196..8061
                     /locus_tag="PLD15_04675"
     CDS             6196..8061
                     /locus_tag="PLD15_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPB63230.1"
                     /translation="MTFINDTSRSSRAQVRPLALERVKLGGFMGRYNNLMRSRTLISQ
                     YELLESTGRLDNFRRAAGKIEGDFKGFFFNDSDVYKWLEAASYALIDNDDPELMEKIA
                     LTIDVIRDAQNAGNDGYLDTYFTFEKESERWTDFKNMHELYCAGHLIQAAIAHRRVTG
                     SEDLFAVALKFVDHIVKMFGKNGRETTTGHPELEMALVELYRETGTREYLELASRLID
                     NRGKGLAGGDEYRIDHVPFRQLKELTGHAVRMLYLVAGAADVFLETGDETLLAVIDRL
                     WADLVGRKMYITGGAGSRHEGESFGEAFELPNRRAYTETCAAIASFFWNWRMYMISGD
                     GKYLDVMERTLYNGVLPGISLDGLKYFYVNPLEDIGNHERKEWYSCACCPPNIARVLT
                     SFGGYMYGLTLDEIRVNFYETSEAVIPFRDGEINISQKTGYPNDGEIELTVSTDLDMQ
                     LSILLRIPGWTEGEFDLQIDGIKQKAKVEKGFIKLTDNWKGKTSILLDLPMEINMITS
                     NPMVRENTYKIAIERGPLVYCAEGRDNEGFSVWALSVPVKKGYDVQMNEELPGGYVTI
                     SGKGIAYDLDEWEGALYKPLAVVKNRGRNVRFTLIPYYAWNNRGSSPMCVWLHIH"
     gene            8087..9406
                     /locus_tag="PLD15_04680"
     CDS             8087..9406
                     /locus_tag="PLD15_04680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB63231.1"
                     /translation="MIHYRAGLMDGVSLEMEKWRAVLTGMGHSVEIVAGNSCPGVDTT
                     IPGIEYNDGQNLALNEKLYEKGEIETRELLEEIRERSEGLLRDFERELSRFDLLVPNN
                     IWSLGWSLPAGLALYMYARDSGKPFISHNHDFWWDRPYYSSPRRGVVEILERYFPPSL
                     DNISHITINSISAGDLLRRRKLHATVVPNVMDFAQPSWTSEKNLQLRRLAGIAENDVV
                     FLQATRVTERKAIELAVELVARFNTLSRVLKGRRLYTGREFSGRAHLVLTGLTEKQAL
                     GYRKKIDALASKLGVNVVDLSKQCSQSQKEFFQSYSIADIVTYPSILEGWGNQLLEAM
                     FARKPVALFKYAVFKSDIEDSGLSFVDLGDSYVLEGGLAKIPDEVFDGAVLNLVDLLF
                     NRQKYLAATENNFSTGSEKFSYGALAGILRELLSNLKAEASKQTGRR"
     gene            9410..10249
                     /locus_tag="PLD15_04685"
     CDS             9410..10249
                     /locus_tag="PLD15_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB63232.1"
                     /translation="MKHVRDIIRVSILITLFVVLSLCGCKSDGLTTVTEVIDGDTIVV
                     ESLEASVRLIGVDAPELRDGSKPAGQYAQEARLFILESLKKGNSKVRLELHGKDSYGR
                     NLAYVFTPDGKMLNTELLRNGLARPLTYEETSHYNDKIVSAYRDAFKNRKGIFSSYNT
                     LKTVEASKIKDNLKPYSQGGFLGKIVWVEFFVTDTSRLSISGKDIIAKVRQEEAELFM
                     PEGFDFDKLYRKTVRVYGEVWEDSSGKASILLRDPAIEIGNISDFVYQRPNIADIVYA
                     ARF"
BASE COUNT         2581 a         2673 c         2650 g         2601 t
ORIGIN      
        1 cagccgtctc cgccgaacac ccagaccgat ttcttgatca gaagatcctt tcgttcctcg
       61 atctccttca ttaactggcg ggccgcttcg ctcttgcagt ccctggatag gacgcttaga
      121 atgtccagag cggcggcctt ggatgccttc gcgtcttctt ttccttcaag ccaggcttcg
      181 aacggcgcct tgacatcttc gggaatgtcc agcaccagaa gttcctgaat gatggaagca
      241 agcttgtttc tgccctgttt gattgccatg gccataccga aaccgtactc ggcgttgtct
      301 tcgaagagag agttggccca tgccggtccg tggccctggg cattcacgca gtaaggagtt
      361 gccggcgccg aggcgcccca gatcgaggaa cagcctgtgg cgttggcgat cagcattcta
      421 tcgccgaaga gctgggtcac gagcttggcg tagggagtct ctccacaacc ggcgcatgcc
      481 ccggagaatt cgagcagggg ctttacgaac tggctgcctt tgagagtctc tacgttcatc
      541 agatctttct tttcggagat cgtgttgacg aaattccagt tggggatctc cttctcgagt
      601 tgcgattcga gcggtttcat gattagggcc ttgttggggg ccggacatat gtctgcacag
      661 tttccgcaac cggtacaatc aagcggcgag acctgtattt tgtactgcag tccttcgaag
      721 gcctttccac cgactgcctt cttcatctcg aaagtcgcgg gtgccttaga ggcttcctcg
      781 gcgttgacga ggaatggcct gatcgccgcg tggggacaga cgtaggaaca ctggttacac
      841 tgtatgcagt tttccagctg ccactcgggg atgtctatgg ctattccgcg cttttcgtaa
      901 gccgtcgttc cgctggggaa ctcaccgttc tctcttccga cgaaggcaga taccggcagc
      961 ttgtcgcctt cctgcctgtt catagtatcg acaaccttct tgatgaattc cggcctgtcg
     1021 gaatcttcaa tcttttcatc taccgcgttt ttccaggcgg ccgggatttc gatctttacc
     1081 agagcgctta taccctgttc gaccgccctg tagttcatct cgacgacttt ttcgcccttg
     1141 cggccatagg atttcacgat ggcgtctttc agatgctcta ccgcgtcctc tatcgggatg
     1201 acgttggcca gcttgaagaa ggccgactgc attatcatgt tgattctatt tccgagaccg
     1261 atttcaacgg ctatcttcgt ggcgtcgatc gtgtagaact tgatatcgtt ttccgccagg
     1321 tatctcttca tgctggccgg aagatgctta tcgagctctt cgaggctcca gccggtattg
     1381 agaacgaagg taccgccctt cttgagtcca tcgagaagat cgtactggtt cacgtaggcc
     1441 ggtttgtgac aggctatata gtcggcttcg tcgatgagat aggtggattt aatgggcttc
     1501 ttcccgaatc tgagatgcga gatcgtcacg ccgccggatt ttttcgaatc gtaggcgaag
     1561 tatccctgcg catagagttt cgtgtagtcg ccgatgatct ttatggcatc tttgttcgca
     1621 ccgaccgttc cgtcggaacc gaaaccccag aacttgcagc ggatggtgct ctccggagcg
     1681 gtgttgatct tgtctttgat ttccagggac gtattggtaa cgtcgtctat gattccgatg
     1741 gtgaagtgat ccttcggggc ggtattcttg agattatcga aaacggcctt gatctgagag
     1801 ggagtcgtgt ctttcgagcc gagaccgtac cttccgccta ccaccagagg ggcgtcttca
     1861 cagttgtaga aaagtgtctt gacgtcttcg tacaaaggtt cgcccagaga tcccggctct
     1921 ttggttctgt cgagaacggc gatctttttg actgttttgg ggagcacgtc gaagaagtgc
     1981 ttcgccgaga agggtctgta gagatgaacc ttgaggacgc cgaccttttc tcccttcttg
     2041 agaaggtagt ccacggtctc ctcgacagtc tccgttaccg aacccatggc aattattata
     2101 ttttcggctt cccgatcgcc gtaatacacg aacggtttgt attcgcgtcc ggtgagcctg
     2161 gatatctcat ccatgtattt ggcaactatg tccgggacgg catcgtagaa tctgttggaa
     2221 gcttctttcg cctgaaagaa gatatccggg ttctgggccg tgcccatagt tttgggatgc
     2281 tccgggttca gggcgtcgtc tttgaaacgc ttgacggctt cataatcgac cagcttcgcc
     2341 agttcttcgt agtccagcac ttcgattttc tggacttcgc tggaagttct gaatccatcg
     2401 aagaaatgca ggaagggaac cctggagttg atcgccgaaa ggtgggcgat cgaaccgaga
     2461 tccattattt cctgaacgct tccggaagcc agaagggcaa accccgtctg cctgacggcc
     2521 ataacgtcgg aatgatcgcc gaatatcgag agagcgtgac cggcgatggc gcgggcgctg
     2581 acgtgaaaca ctcccggtag aagttcgccg gcgattttgt acatgttggg aaccatgaga
     2641 agaagtccct gcgatgccgt gaaggttgtg gttaaagctc cggccgcaag agagccatga
     2701 accgcccccg ccgcaccggc ttccgattgc atttctataa catcgacgac ctgaccaaaa
     2761 atgttcttca acccgttcgc cgcccaggca tctgcgagct ccgccatggt cgaagacggg
     2821 gtgatgggat aaatggccgc tacctctgtg aaagcgtaag cgacgtgggc ggcagccgta
     2881 ttaccgtcca ttgttttcat tactcgtgcc attctttacc tcctggaatc cttgaagttt
     2941 tggctgatct atatggttcg cgatatacat gaatttagtt cagtgctccg gtcactggtc
     3001 atctacatta tagaacaagt tgcaccagct ttcatggaca gatttttttc agcctgtgaa
     3061 ttgcattaga caacctgccg gaaaatcgag aataccatta agtaaaaaat agaatataca
     3121 acgagtaaaa gtgaagtcat agatgactat atatcaaaat attttcccga cttgcaaaat
     3181 aaattgatat atgatataaa aatattatcg aaattcaaag gagtgatttc atgacattat
     3241 cgaggaaaca gatagcgatt ataatcgggg ccgccgttct cttcacgggt ctctttcttc
     3301 cggcgatttc tctgaaggcc aacaatcctc tggctaacat cgccggttcg agcaactata
     3361 ttcaggcaga tcaggcttac ggcgtgatac ttcttttgct ttcgttgatc gtggtggcgc
     3421 tggcgatcgt gaagaaattc cagtgggcgt tcatagccgc cgttgggact ctgggaatac
     3481 tcgtcttcag gctcgtcacc tttctcacgg atatctcgaa gttcaactct cttcagagca
     3541 gcatgaatcc aggcccgttt tcgcccgagt tcgacggagt catgggtatg acacaagaga
     3601 ttgcagtcgg tatttctctg aactggataa gctggatagt catgtttgca ggttctataa
     3661 taacgatcgt cgtactgctg agagaggata aaatccgtga aatcaaataa aaatcgagca
     3721 gaagaatctc gagtcttaca gttccacctg gtcgaaccac tcgataagat cggcgcgttc
     3781 cttattttcc gggaggctgg caagggttcc tgtcaggctc tccctggaaa actctatacc
     3841 cttcagggaa cttctcagca attctatgaa atctacgtcc atagaatcgg aataaacgac
     3901 agcttccctt atcattccct ttatgacgtt caatcctatc tctattccgc cccagacgaa
     3961 gcggttttcg atctcgaagt cgaactcggg actctttccc agcagccatt ccctggatga
     4021 atacttcgag taaagagttt tgacctcttc ccgggaggcg atttcatcgt agtccagaat
     4081 cctgtctata tgaccgtact cctctataaa gccatcgtac atggcccttt tcattatctc
     4141 tatcgttaga tcgggtttga gatctttcag attcaccact ctcgatgcga cggatctgac
     4201 gcctttagat tccattttcg ccttcgagac gttcaggtac atcgaaagcc tgctcatatc
     4261 gacgtctata agcaccgttc catggtgata cgatgcctgc cgtccatgat agaaggcgtt
     4321 gccggaaaac ttcttgccct cggccagaat atcgtttcgc ccgctgaaat tggcttcgat
     4381 ccccagccct ttaaccgcat cgactattac ggcgagctgc ctgtggagat tgtactctaa
     4441 ccggggaagg aggaaagtga agttgagatt tcccagatcg tgatagacgg cgccaccgcc
     4501 agacaaccgc cgggcgagtt taccgccgtc cctttcgagg ttctctatgc ggcactcccg
     4561 ccaggcgttc tggttgcgcc cgattaccac ggtgtgggcg ttctgccaga ggtacagcac
     4621 ggcgttatcg cgcgcgtaat tgtcgagcaa atattcttct atcgaaagat tcaaccacgg
     4681 gtcgaaagaa ttcgactcta tgtaagcggt cttcaacgtt tcttatttac ctccgagtcc
     4741 gagagagccc gactcttttc tcacgtacat gtactgactg ctggacagat aactcctcgg
     4801 atctttacac tggttcttgg aatcgactat ctcgaaatgc agatgcggac cggttgcccg
     4861 gccggtctct cctaccctgc cgatcaactc acctctggcg acccgctgtc cagaatatac
     4921 acttatgtgg ctcagatggg cgtaacgtgc tttcgaacca tcggcgtgaa gaatgattat
     4981 gttcaggccg taaccaccgt tgtcggcggc ctcgatcacc gtcccatctc tggctgcgaa
     5041 aaccggcgca ccctcctgaa cggctatatc cataccggag tggaaagttg aagcgcctgt
     5101 gaagggatcc tttctccagc caaactccga ggagatcttc ccgtaaaccg gccaggaata
     5161 ctgggtactg atgttcatgt tactgcagac actcacgatt tccagcggaa tgaacaatct
     5221 ctgacccgtg tagatcttcg aagagtaact caatctgttg gcgctcatga tgtcctcaac
     5281 cagagcaaaa aagagcttgg cgatatactc cggagagtcg ccgtcctgaa catcgtaaaa
     5341 gaaaccgtct cttacgggaa tgtagatcgc ctgacccgat tcgagacacc tgggatcgtc
     5401 gatgcagttg aaatccagaa ccgtactgat tctgatgttg taatccctga taatgttgaa
     5461 caatttgtcg ccgggcttta cgagataacg aacataactg ttaccgagag cggtgcaaag
     5521 gaaaaccaga accacaagaa agataagtcc ttttactctc atttgaaacc cccaaaccgt
     5581 cagtcttgtt atgactcgca aaatacagaa atagaaaatg atgataaaat aataccacaa
     5641 actccccatc caaaaacagc gaacgatcgg gatatttcac aggtcgaaaa aaccaaaaca
     5701 ctgttcttca ggagaaaaag acctgcacaa acgccagaaa aagctcattg aagcaggatt
     5761 cttctcagaa tctccacccg gagctcttaa agaacctctt caatggtggg caaaaagccc
     5821 agttgatctc cggccttttc ctttccgtcc accctcagga actcaccctg ttccatcttt
     5881 atctttctga cctgttccat cctcttccac tcgccttttt catttttagc tatcgtgcag
     5941 aaaacgaaaa catcgcattt ttcgaggatc tgttcccggg tccattcttc gtcgaactcg
     6001 tgtgattccc caatatttgg atgaatgagc gcggcttcta gaaaccatgt cccctttctg
     6061 tccgcgtagc ttgccgcggt gacccagtaa tctatctttt caaacatctt tatcccctcc
     6121 atcaaaagca tacataagtt atagtctttt ctgctactct agaagggaga gcgttttttg
     6181 tgggaggtgt gaagggtgac ttttataaac gatacgtctc gatcatccag ggctcaggtc
     6241 aggccgctgg cactggagag ggtgaaactg ggtggtttca tgggcaggta caataacctt
     6301 atgagatcca gaacactaat ctcccagtac gaactgctgg aatctaccgg caggctcgac
     6361 aatttcagga gggccgcagg taagatcgaa ggagatttca agggcttttt tttcaacgat
     6421 tccgatgttt acaaatggct cgaggccgct tcttatgctc tcatagacaa cgacgatccc
     6481 gaactcatgg agaagatcgc cctgacgatc gatgttattc gcgatgcaca gaatgccgga
     6541 aatgacggct atctggatac ctatttcact ttcgaaaaag agtcagagcg ctggacggat
     6601 ttcaagaaca tgcacgaatt gtactgtgcc ggtcatctta tacaggccgc gatcgcgcat
     6661 cgccgggtca cgggaagtga ggatctcttc gccgtcgctc tgaaatttgt cgatcatatc
     6721 gtcaaaatgt tcggcaaaaa cggtcgcgag acaaccacgg gccatccgga actggagatg
     6781 gcgctcgtgg agctttacag agaaaccgga acgagagaat acctggaact cgcatcgaga
     6841 cttatagata acaggggaaa ggggctcgcc ggaggcgatg aatacaggat cgatcatgtt
     6901 ccgttcaggc aattgaaaga gttgacgggc cacgcggtga gaatgcttta tctggtggcc
     6961 ggcgccgccg atgttttcct cgaaacgggc gacgagacac ttctggccgt tatagaccgc
     7021 ctatgggcag atctggtcgg gagaaagatg tacataaccg gtggcgccgg gtccagacac
     7081 gaaggggagt ctttcgggga ggccttcgag ctgcccaaca ggagagcgta caccgaaacc
     7141 tgcgcggcca tcgcgagttt cttctggaac tggcggatgt acatgatatc cggggatggc
     7201 aaatacctcg atgtaatgga gcggactctc tacaacggtg ttcttccagg tatatccctc
     7261 gacgggttga agtacttcta cgtgaacccc ctggaagata taggcaacca cgagagaaag
     7321 gaatggtact cctgtgcctg ctgtccgccg aacatagcca gagtgcttac ctctttcggg
     7381 ggttacatgt acggtctcac tcttgacgag ataagggtga acttctacga aacgagcgaa
     7441 gccgtcatac ctttcagaga cggagagatc aacatatctc agaaaaccgg ctatcctaac
     7501 gatggagaaa tcgaactcac ggtctcgaca gatctcgata tgcagctttc catactgttg
     7561 aggataccgg gctggacgga aggcgagttc gatcttcaaa tagacggtat aaaacagaag
     7621 gcaaaggttg aaaagggttt cataaaactc acagacaact ggaagggaaa gacctcgata
     7681 cttctcgatc tacccatgga gatcaacatg atcacctcca acccgatggt cagggagaac
     7741 acctacaaaa tcgccataga aaggggcccg ctggtttact gcgcagaagg aagagacaac
     7801 gaagggttca gcgtctgggc gctgtccgta ccggtgaaaa aaggttacga cgtgcagatg
     7861 aatgaagagt tgcccggcgg atacgtgact atctccggaa aagggatcgc ctatgatctc
     7921 gatgaatggg aaggggcgct ctacaagccg ctggctgtcg tcaagaacag gggtagaaac
     7981 gtgcgcttca cccttatacc gtactacgcc tggaacaacc ggggaagctc gcccatgtgt
     8041 gtctggttgc atatccactg agggggtaag agttataaga attggattga ttcactacag
     8101 agccggacta atggacggag tctctctaga gatggagaag tggcgggctg tgctcactgg
     8161 catgggacac tccgtcgaga tcgtagcggg taattcctgc ccgggagtag atacgaccat
     8221 tccaggcata gaatacaacg acgggcaaaa cctcgcgttg aacgaaaaac tgtacgaaaa
     8281 gggtgaaata gaaacgcggg aactgcttga agaaattcgt gagcgaagcg aaggacttct
     8341 tcgcgacttc gagagggaac tctcccgctt cgatctgctc gttcccaaca acatctggtc
     8401 gctgggctgg tccctgcctg ccgggctggc cctttatatg tacgccagag actccggcaa
     8461 gcccttcatc tcgcacaatc acgatttctg gtgggacagg ccttactatt cgagcccccg
     8521 ccgtggagtc gtcgaaattc tcgagaggta cttcccgcca tctctggata acatatctca
     8581 tataactata aactccatct ccgcaggtga tctcctgaga agaagaaagc tccacgccac
     8641 ggtagtgccg aacgttatgg atttcgcaca gccgtcctgg acatccgaaa agaaccttca
     8701 actgagaagg ctggccggta tcgccgagaa cgacgtggtc tttcttcagg ctacgagggt
     8761 tacggagcgc aaagccatag aactcgccgt ggaactggtg gccaggttca acaccctctc
     8821 tcgcgtgttg aagggcaggc gtctctacac gggtcgggag ttttcgggaa gggctcacct
     8881 ggttctcaca ggcctgacgg agaagcaggc tctcgggtac aggaagaaaa tcgacgctct
     8941 tgcatcaaaa ctgggcgtga acgttgttga tctttccaaa caatgttctc agtcgcagaa
     9001 agagttcttc cagtcctaca gtatcgccga catagtaacc tatcccagta tcctggaagg
     9061 ttggggcaat cagctcctgg aagccatgtt cgcacgcaaa cccgtcgcgc ttttcaaata
     9121 tgcggtcttc aaaagcgaca ttgaagacag cgggctttcc ttcgtggacc ttggagatag
     9181 ctatgtattg gaagggggac tcgcaaaaat cccggatgaa gtgtttgacg gggccgttct
     9241 gaacctggtc gatttgcttt tcaaccgaca aaaatacctg gcagccaccg aaaacaactt
     9301 ctccacagga agtgaaaagt tcagttacgg agcactggcg ggaattctca gagaactcct
     9361 ctctaatttg aaggcagagg cctccaaaca aactggaaga aggtgacaaa tgaagcacgt
     9421 ccgggatatc atcagagttt ccattctcat aacgcttttc gtggttcttt ctctgtgcgg
     9481 ctgcaaaagc gacggattaa cgaccgtaac cgaagtcata gacggcgata cgatagtcgt
     9541 agaatctctg gaagccagcg taaggctcat aggcgtagac gctccggaac tcagggacgg
     9601 cagcaaaccg gccggccagt acgcccagga ggccaggctt ttcattctgg aaagtttaaa
     9661 gaaagggaac tcgaaggtca ggctcgaact ccacgggaag gacagttacg ggcggaacct
     9721 ggcctacgtc ttcacccccg acggaaagat gctgaacacg gagcttctgc gcaacggtct
     9781 cgccagaccc cttacgtatg aagagacttc ccactacaac gacaagatcg tatcggccta
     9841 tcgcgatgct ttcaagaaca ggaagggaat cttcagctcc tacaacaccc tcaaaaccgt
     9901 tgaagcctca aagatcaaag ataatcttaa gccttacagc cagggcggat tcctgggaaa
     9961 gattgtatgg gtggaattct tcgtcaccga tacatctagg ctttctataa gcggaaagga
    10021 tataatagct aaggtccgac aggaagaagc ggagctcttc atgccggagg gattcgattt
    10081 cgataagctg tatcgaaaaa cggtgagagt ttacggagaa gtctgggagg acagctccgg
    10141 aaaggcgtcg atactcctta gagatccggc catcgagatc ggcaatatct cggatttcgt
    10201 atatcaaaga ccgaacatcg ccgatatagt ttacgcagct cgattttgaa gcttttagag
    10261 aaccttctct tttatcttta ataaacccga cacttcaact cgtgaatact ttcaattcca
    10321 gaccccggat gttcaaataa ccacgcttta ttattgactt atccccgaca agtttaggtt
    10381 tattttctcg ctgcgtatgt cccttattag ctaagacaat ccatgttaac ccgaaagact
    10441 ctctatctta ttcaccaaaa gaaaatcgcg gggatatact tcttccaaga acgattcgag
    10501 cctgc
//
LOCUS       DAOOLY010000063        10503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7188_length_10503_cov_3.932332, whole genome shotgun sequence.
ACCESSION   DAOOLY010000063 DAOOLY010000000
VERSION     DAOOLY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7188_length_10503_cov_3.932332"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLD15_04690"
     CDS             complement(<1..222)
                     /locus_tag="PLD15_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB63233.1"
                     /translation="MNYRELSQVCGLKYDTVRNYAKVLIDEGLVKAIDESTVSVVKKI
                     PQYTASGFTIKEAAHKAFEDVYRSSGSEEV"
     gene            419..1630
                     /locus_tag="PLD15_04695"
     CDS             419..1630
                     /locus_tag="PLD15_04695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63234.1"
                     /translation="MEIRKLDNSYRERIIEIFTICFDYKFDIDEDGPFFDDPSLWECV
                     WGAFDGQTLIASYISYKQRVKIRNRAIECRYLDGVATLPEYRNMGIIKDIFMRDCADA
                     LDNGINIMMLDPFKHDYYRKLGFETALENLNLSFDYSLLSREMVENDLRIVSGMLFQK
                     DSMKSLVEAALEKLWEESRYAEVKEISAYSQGIYREKDLLCAVALDDDDLPHGLILYS
                     KKERKMNIRRFSFTDLQGFYALKKFLLNHRDQLSSFEIRRVPPDFPVELLLHSGWQAG
                     KFAELKDFSSRMMRILSPVAVISELMNKKIPDRVIIEIEDRNLPQNNLSLSVGNGEVV
                     TSTDRHGLSISISDLVPIVTGRISASRLWRMGKLGKGECGKLSWGITEVPLDVQILDS
                     LFPRITTHNVH"
     gene            1651..2427
                     /locus_tag="PLD15_04700"
     CDS             1651..2427
                     /locus_tag="PLD15_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63235.1"
                     /translation="MRRVFLFLSLLSVCMAFSFVPLEEPITLGQFGFVDDVKTVKVKI
                     TPIGAQTPESQSYELTFDENGFITSQNMGEIAVEYRYDDQKRLMTKLFVDSSGHLLQR
                     ISIEYAGDSYTAIAYDGSNLEVGRTQYIFDIQNITLAFSNTSESSSSRNTLYFDSNYM
                     NYRLVSYITERVEESDLYVNMVVEIELDYNEFGQFKGQKMIVRVRNEGQERSMEITTR
                     ADWLLTDEKGNITSEIQRTEYSDGSNPPEEFIYSREFTYY"
     gene            2534..4195
                     /gene="hcp"
                     /locus_tag="PLD15_04705"
                     /gene_synonym="priS"
     CDS             2534..4195
                     /gene="hcp"
                     /locus_tag="PLD15_04705"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPB63236.1"
                     /translation="MFCYQCSETMKGTGCTVRGVCGKEPEAASLQDLLIWLLKGVSFW
                     GNGARKLGVRSEEVDLFVAEGLFTTITNVNFDPLSLGKKIEHTLKIRDRAEALFRKAF
                     ADKNGKDYTGTVPEAASWKLAGGIDVYEMKGSEVGVMETKDPDIRSLRELLVYGLKGI
                     AAYADHAYILKHADDSVLAFLQEALAATLDDSLSVNDYVNLVMKAGEHAVKAMALLDG
                     ANTSTYGNPEVTSVFTGKLEGPGILVSGHDLLDLEELLKQTEGKGINIYTHGEMLPAN
                     AYPGLKKYRHLVGNYGTSWYNQQKEFEEFRGPIVMTTNCIQKPVDTYKDRIFTTGLVG
                     WPDVSHIPNRTGKQPKDFSPVIEKALEIGDIGIKHGKHIVVGLAHDQLSKVADKIIDA
                     VRSGKIKKFVVMAGCDGHAKERQYYTDLAGALPGNTVILTAGCAKYRYNMLELGDIGG
                     IPRIVDAGQCNDSYSLAVTALKLKEAFGLEDINDLPIEYDIAWYEQKAVAVLLALLYL
                     GVKGIRLGPVLPAFVSPNVLKVLVDNFNIRPITNVKEDLASIIGR"
     gene            complement(4178..5143)
                     /locus_tag="PLD15_04710"
     CDS             complement(4178..5143)
                     /locus_tag="PLD15_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB63237.1"
                     /translation="MVAFLNTYAIAGSFFSQVRSAFKMIEQLFISSRQSKDYSIREDG
                     IHFPGGMVEGYLIIDYDVEYSRVDRETLTMDIYFPQKMKEESPTVIYVHGGAWASGNK
                     ESGPGLLIIAELVKNGYLVAAIDYRLAPAHIFPAQIMDVKTAVRFLRQNAAVYGIDAS
                     RIGAFGTSAGGHLVSLAGLTPDYDLFRNDQYKEVSDDLMAVANLFGPTDLESLFTGLE
                     KMLAEKIFGKGDDALKVASPVEYVRGDGPPFLIVQGDKDAVVPPYQSEEFYHSLVDSG
                     NYTELLIVKNAGHGFVPSGGKISPSLLEIAGIVVEFFDRFLSADY"
     gene            complement(5211..7397)
                     /locus_tag="PLD15_04715"
     CDS             complement(5211..7397)
                     /locus_tag="PLD15_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63238.1"
                     /translation="MKKILFIIFLFVSMIAFALDTDTLFNNMLSRSQDFVAFTGAGFE
                     ARFIPAYAVADGQDGQIEIEEDLYLLGLFVFRSERSMNIQLDSIQIMNATSDERIEDQ
                     IFRVEELFVPIQLPIVLPSGGIIVLTSQVALQDYFMDVRVQNILLPWRVRTVEPGRTV
                     EIAPVSIEVQPPSTNVADLEGYESINENIADLNERLNFLQDNVEGLNLQFRRLNTLFL
                     DTQIALAEYKASVDNSVIDLQEHRFIFEKRVEALESALSTTTFDSGALDSLKTSIENL
                     NTEMQGIELELQIIEEMRSSHEELKTLLGEQGKTVEELRVAIGSIPSSTSDPEKLSSI
                     EKAIEENRVALEALGGDLQNVKVSLADDLEIIKTSSLELIEAHGRIDERIIELEREIA
                     RVSSVSEDAASSLSEALFSMNGTLEQFESFKNIVMDELTRSYEESIASLGDMESRLSA
                     IETSLADIESLGTELSNQREELRAYEDILNRSIARIDNIDKSVEELEELAQSLDSVLK
                     NLTPSSHDSLNSFLGNLEEQIETLRDSVGSLSIGFVRMNSELIQVKESIPPEGVSPQT
                     FSQTVFEFNSKISDIETIFTTLEAKIGGLNVSLANIDKRLQAAAVEIDSLKGNFDGSV
                     TAIDNNLKSIQKLQSESAQLKMLIEENSRNLATFRREIESRLDSTLVSREEIQMIVDE
                     AVSAAKEETNREISSLKRSNNIWLTIAVLSSVAAIVMGVLNIMEIP"
     gene            7918..9594
                     /locus_tag="PLD15_04720"
     CDS             7918..9594
                     /locus_tag="PLD15_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63239.1"
                     /translation="MKKGFLLLLLAMILVSSLAIGQVYDRKETLYAGGGLWSPPNNWN
                     PFTPWAIMTGTNGLIYEFLFMFDPLSNEMIPWLAVDGGWIDGKTYELKLRDNVFWTDG
                     EKFDAEDVKFTFDIAKKYPGVHYSSMWNWMKEVEIIDTLTARVHFTEPLYQQWSFQLY
                     QLPMVPEHIWKNKTEAEILTGANEGPIGTGCYLAEGYGQDRMIYLRNENWWGIEQLGI
                     KPTPKRIVYLTVSGNNVALGMIFKGELDISNFFLPGVPAVKAAYGIHTYFDGAPYMLS
                     DNTAVLFLNNSRKPMDDVNFRKAVAWAINSDDIVTRVFENQVIRSNPLGFLPIDAWMK
                     YYDEKVVEQYGFKYDPVVSKKTLADAGYKDINGDGFVEAPDGSKVELSIIVPFGWTDW
                     MESIKIIANNLKTVGINAKAEFPDYSRYQDELYSGRFDMAINNFNSNLSNTVWSYYYW
                     LFWDIREQQTQGNYGKYNNPKAFELMGAFDRTPVDDYEAGQKIMLELEELFLKEIPYV
                     PLWFNGMWFQASTSVWSNWPSKHGPHYYPCTWNGKWQLGGIFMLSALKAE"
     gene            9681..>10503
                     /locus_tag="PLD15_04725"
     CDS             9681..>10503
                     /locus_tag="PLD15_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63240.1"
                     /translation="MGAYFRRKIVIYLLTFIFAVTIDSMIPRFMPGNPIQNLLSRFST
                     RSDTTEVIARYLNEIYGLDKSPLQQYFGFWAGLFRGDLGVSIYVTGAPVARVIARALP
                     FDLALLIPAIMLSYIAGNKFGAFAARKKRLDGIVLPVWYVLTATPYMWLGILLAWFFG
                     VALDILPIAGAYSFSMVPNWSWGFIWDFFKHWILPFSSLFIVQFGGWAIGMRNMIIYE
                     LEAEYCRYLESLGASRKLIRRYAYKNAVLPQITGLALQLGVIVAGALATEVVFSYP"
BASE COUNT         2794 a         2487 c         2514 g         2708 t
ORIGIN      
        1 gacctcttcg gatccagagc ttcgatacac atcttcaaag gctttgtggg cagcctcttt
       61 aatggtaaag ccactggctg tgtattgggg aatctttttg acgaccgaaa ccgtgctttc
      121 gtcgatcgcc ttcaccagac cctcatcgat aaggaccttt gcgtagttcc tgacggtgtc
      181 atacttaaga ccacacacct gagaaagttc cctgtagttc atggttaccc ctccatgctt
      241 cgattatcat acgccatata aagtatagca gagtgtccgc aaattccttc tcaatatccg
      301 tttctaacct ttcaaaaggt tttcggacat accatctggt atcatagttg atatgcaaat
      361 cgagtcggta ccgtttgaaa ctgttaaccg acgggtggag gaggtgtatt cgaaaaatat
      421 ggaaatcaga aaactcgata acagctacag agaaaggatc atagaaatat tcaccatttg
      481 cttcgattac aaattcgata tcgacgagga tggacctttc ttcgacgatc cttccttgtg
      541 ggaatgtgtg tggggcgcct tcgacggcca gacgcttatt gccagttaca taagctacaa
      601 acagcgagtg aagatacgta acagggcgat cgaatgccgt tatctggacg gtgtcgcgac
      661 tcttcctgaa tacaggaaca tgggtataat caaagatatc tttatgagag attgtgcaga
      721 tgccctcgac aatggaataa acatcatgat gttggatcct ttcaaacacg actactaccg
      781 taagctcggc ttcgaaacag cacttgagaa cctgaacctg agtttcgatt actccctcct
      841 ctcgagagag atggttgaaa acgatttgag aattgtgtcg ggcatgctct ttcaaaaaga
      901 ctctatgaag tcactggtgg aagccgctct cgaaaagcta tgggaagagt cgaggtacgc
      961 agaagtcaaa gaaataagcg cctacagtca gggcatttac cgcgagaagg atcttttgtg
     1021 tgccgtggcg ctcgatgatg atgacctgcc gcatggtttg attctgtatt ctaaaaaaga
     1081 acgtaaaatg aatatcagac gttttagttt cacagatcta cagggtttct acgctctgaa
     1141 gaagtttctg ctgaaccaca gagatcaact gtcatccttc gaaatcagaa gggttcctcc
     1201 cgacttcccg gtcgaactcc ttctacactc tggctggcag gccggcaaat tcgccgaatt
     1261 gaaggatttt tcttcgagga tgatgagaat cctatcgccg gtcgcggtca tttcggaact
     1321 gatgaacaag aaaattcccg atcgtgtcat aatcgaaata gaagacagga acctgccaca
     1381 gaacaacttg tcgttgtccg tcgggaacgg cgaagtggta acatctactg atcgacacgg
     1441 tctttcgata tctatctccg atctggtacc tatcgtgacc ggcaggattt cggcatctag
     1501 actgtggaga atggggaaac ttggcaaagg ggaatgcgga aagctctcgt ggggcatcac
     1561 cgaagttccc ctggatgtcc agatcctgga ctctctcttt ccccgtatca ctacccataa
     1621 cgtacattga gagtatagag gtggtttgtt ttgagaagag tttttctgtt tctttcgtta
     1681 ttgtccgtct gcatggcctt ttctttcgtt ccgcttgagg aacctataac acttgggcag
     1741 ttcggttttg tggacgatgt gaaaaccgtt aaagtgaaga ttaccccgat cggggcacaa
     1801 acaccggaat cccagtccta cgagttgacg tttgatgaaa acggtttcat cacctcccag
     1861 aatatgggag agatagccgt ggagtacagg tacgatgatc aaaaaaggct tatgacgaag
     1921 ctctttgtag attcctccgg ccatctgctt cagaggatat ccatcgaata cgccggagac
     1981 agttataccg ccatcgcata tgacggttct aaccttgaag tgggccgaac acagtacata
     2041 ttcgatattc agaacatcac actcgccttt tccaatacat cagagagcag ctcttcacgg
     2101 aacacgcttt acttcgattc gaactacatg aactaccgtc tggtttcata cataaccgaa
     2161 agagttgagg agagcgatct ttacgtcaac atggtcgtgg agatcgagct cgactataac
     2221 gagttcggac agttcaaagg tcagaaaatg atcgtaagag ttaggaacga aggtcaggaa
     2281 agatccatgg aaatcactac cagggccgac tggttattga ctgacgaaaa ggggaatatc
     2341 accagcgaaa tacagagaac agagtattcc gacggcagca accctccaga ggaattcatc
     2401 tattccaggg agttcacgta ctattgacga cgattcgctt caggtagcaa atccggccac
     2461 gtaacatatg ttacagacct agaatgcacc taccggttac tatatttgta accagtaaaa
     2521 ggagggatgc aaggtgtttt gctatcaatg ttcagagacc atgaagggaa cgggttgtac
     2581 tgtcagaggg gtctgtggaa aggaaccgga agcagccagt ctccaggatc tactcatctg
     2641 gttgctcaag ggagtctctt tctggggaaa cggcgccaga aaactcggtg tccggagtga
     2701 agaagtggat ctcttcgtgg ccgaaggact tttcacaacc ataaccaacg tcaacttcga
     2761 tcctttgagc cttggaaaga agattgaaca tacactgaag atcagggaca gggccgaagc
     2821 tctgttcaga aaagccttcg cagataagaa tggaaaggat tacacgggta ccgtcccaga
     2881 agctgccagc tggaaattgg ccggggggat cgatgtttac gaaatgaagg gttccgaagt
     2941 tggcgtaatg gaaaccaagg atcccgacat aagatctttg agagaactgc tggtctatgg
     3001 cctaaaaggc atagccgctt acgcagacca cgcatacata ctgaagcatg ccgacgattc
     3061 ggtactcgct tttctacagg aggcccttgc cgcaactctc gatgattctc tttccgtaaa
     3121 cgattacgtc aatctcgtca tgaaggccgg agaacatgcc gtgaaggcca tggccctgct
     3181 ggatggggcg aacacttcca cttatggtaa tcccgaggtc acctccgtct tcaccggtaa
     3241 gctagaaggt cctggaatac tcgtcagcgg tcacgattta ctcgatcttg aagagctttt
     3301 gaagcagaca gagggaaagg ggatcaacat ctacactcac ggtgagatgc tcccggccaa
     3361 cgcttatcct ggcctgaaga aatatcgaca tctggtgggt aactacggca cttcctggta
     3421 caaccagcaa aaggaattcg aagagttcag gggtccgatc gttatgacca ccaactgcat
     3481 tcagaaacca gtagatactt acaaggacag gatcttcacg acgggtcttg tgggatggcc
     3541 ggacgtctct cacataccca acagaaccgg taaacagccc aaagacttct cgccggtgat
     3601 cgaaaaagct ctggaaatag gtgacatagg catcaaacat ggaaaacaca tagtggtagg
     3661 cctggctcat gatcagctat cgaaagtggc agacaagata atcgacgccg ttagatcggg
     3721 aaagatcaag aagttcgttg tcatggccgg ctgcgacggc catgctaaag agagacagta
     3781 ctacacggac cttgcaggcg ctttgccggg aaataccgtt atactcaccg ccggttgtgc
     3841 taagtaccgc tacaatatgc tagaacttgg agatatcgga ggcattccaa gaatagtgga
     3901 tgccggtcag tgtaacgaca gctactcact ggcagtcacc gccctcaaac tcaaggaagc
     3961 tttcggactg gaggatataa acgatcttcc gatagaatac gacatagcat ggtacgaaca
     4021 gaaagccgtg gccgttcttc tggctttgct ttacctggga gtcaagggaa tcagattggg
     4081 gccggttctc cctgccttcg tatcgccgaa cgtattgaag gtgctggtcg ataacttcaa
     4141 cataagacct atcaccaacg taaaagagga cctagcttca ataatcggcc gatagaaatc
     4201 gatcgaagaa ctccaccacg ataccggcta tctcgagtaa tgaaggactg atttttccac
     4261 cgctgggcac aaaaccgtgc ccggcgtttt tcactatgag gagttcggta taattaccgc
     4321 tatccacgag cgaatgataa aactcttcgg actggtacgg gggtacgact gcatccttat
     4381 cgccctgtac tatcaaaaaa ggcggaccat cgcccctaac atattccaca gggctcgcta
     4441 ccttcaaagc gtcgtccccc tttccgaaaa tcttctcggc gagcattttc tccagaccgg
     4501 tgaagagact ttccagatcg gtaggaccga agaggttggc aacggccatc agatcatcgg
     4561 atacttcttt gtattgatcg ttcctgaaaa gatcgtagtc gggagtcaat cctgccagcg
     4621 agaccaggtg tccacctgcg ctcgttccga aggcaccgat tctggaagca tctatcccgt
     4681 aaacagccgc attttgcctg agaaatctaa ctgctgtctt cacatccata atctgagccg
     4741 gaaatatatg agcaggagcc agtctgtaat cgatggcggc aaccaaatag ccgttcttga
     4801 cgagctcggc tattatcagc agacccggac cggactcttt gttaccgctg gcccaggctc
     4861 caccgtgaac ataaatcacc gtgggagact cttccttcat tttctgggga aagtagatat
     4921 ccatagttag agtctctctg tctactctcg aatactcgac atcgtagtcg atgatgagat
     4981 acccttccac cattccaccg ggaaagtgaa tcccgtcttc tcttatgctg tagtccttcg
     5041 attggcgcga agagatgaag agctgttcga tcatcttgaa agccgatctc acctgcgaaa
     5101 aaaaggaacc ggcaattgcg tatgtattta gaaacgcaac caatacgagt agtggaagga
     5161 atcttttcat tatacttatc ctgattcgac ttcaaaccgg gctaagaaac tcagggtatc
     5221 tccattatgt tcagcacacc catgactatg gccgccaccg aagagagcac cgcgatagtc
     5281 agccagatgt tgtttgagcg cttcaaagaa gatatttctc tgtttgtctc ctctttggct
     5341 gcgctaacgg cttcatcgac gatcatttga atctcctctc tgcttaccag agtggaatcg
     5401 agtctgctct cgatttccct acggaatgtt gccagattcc tggaattttc ttcgattagc
     5461 atttttagct gtgctgactc gctctggagc ttttgaatgg atttcaggtt gttatcgatt
     5521 gccgtgaccg atccatcgaa gttacccttc aagctgtcga tttcgacggc ggctgcctgg
     5581 agacgcttat cgatattggc aagcgagacg ttcaggccgc ctatcttcgc ttccagcgtg
     5641 gtgaagatcg tttctatgtc cgatatcttg ctgttgaact cgaaaactgt ctgcgagaaa
     5701 gtctggggtg aaacgccttc gggagggatc gactccttga cctgtataag ttccgaattc
     5761 attctcacga agccgatcga taagctccca acggaatctc tcaaagtctc tatctgttcc
     5821 tctagattgc ccaaaaaact gttcagggaa tcgtgggaag aaggcgtaag attcttaaga
     5881 acgctatcca ggctttgcgc aagttcctca agctcttcga cgcttttgtc gatattgtcg
     5941 atgcgggcta tagatctgtt caaaatatct tcgtaagctc tcaactcttc ccgctggttg
     6001 ctcagctccg tcccgagaga ctctatatcg gccagcgaag tttcaattgc ggatagcctc
     6061 gattccatat cgcccaaact tgcgatcgat tcttcatacg atctagtgag ctcatccatc
     6121 acgatgttct taaagctctc aaactgctcg agtgtaccgt tcatggaaaa aagcgcttcc
     6181 gaaagagagc tggccgcatc ttcactaacg ctactcaccc tggcgatctc ccgctccagt
     6241 tcaatgattc tttcatcaat tctcccgtga gcttcgataa gttccagcga agaggtcttt
     6301 ataatttcta gatcatcggc aagggagact tttacatttt gaagatcccc ccccagagct
     6361 tccagagcaa cgcgattttc ttcgatcgcc ttttctatac tcgaaagttt ctctggatca
     6421 gacgtcgatg agggaatact tccaatagct actctcagct cttcgaccgt ctttccctgc
     6481 tcgcccagca aagttttgag ctcttcatgt gacgatctca tctcttctat gatctgaagc
     6541 tcgagttcaa tcccctgcat ctcggtgttc agattttcga tagaagtctt cagcgaatct
     6601 agcgcgcctg agtcgaaggt agttgtagag agagccgatt cgagcgcttc cactctcttc
     6661 tcgaagatga atctgtgctc ttgaaggtcg atcacgctgt tgtccactga ggctttgtat
     6721 tcggccaggg caatctgtgt atcgagaaag agagtgttca atcttctgaa ttgaaggttg
     6781 agaccttcga cgttgtcctg gaggaagttc agtctttcat tgagatcagc gatgttttcg
     6841 ttgatagact cgtagccttc aagatctgcc acgttggtag atggaggttg cacctctatg
     6901 ctcaccggcg ctatttcgac tgttctaccg ggttcaacgg ttctgactct ccagggaagc
     6961 agtatattct gaactctgac atccatgaaa taatcctgga gggctacctg gctggtgaga
     7021 actataatcc caccggaagg caaaacaatt ggaagctgga tcggtacgaa gagctcctcg
     7081 actctgaaaa tctgatcctc gattcgctca tcggaggtcg cgttcattat ctggatcgag
     7141 tccagttgta tgttcatcga tctttccgat ctaaaaacaa ataaccccag caaataaaga
     7201 tcttcctcga tttctatctg cccatcctgc ccgtcggcga cagcgtaggc cgggatgaac
     7261 ctggcctcga aacctgcacc ggtgaaggct acaaagtcct ggcttctgga aagcatgttg
     7321 ttgaagagag tatcggtatc caaggcaaaa gctatcattg aaacaaaaag aaagatgatg
     7381 aaaagtatct ttttcataat ctatacctcc gataagccat aaggcccgtt attgtatatc
     7441 agtatttctt gatcccctat cggtcataat aatagactat catatccctg cctgcagttg
     7501 taagtaagac cacttctcgg gattttctgt tcttcgaaac tggtttgtct tttaattatg
     7561 aagactgcgg cagaagatat gaaaatcatt tacatatttt tgccggtcaa atacgactat
     7621 cttttatgat cagcgtaatc tcaaaatcat agttagttat tgaatggtca actataaatt
     7681 atcttattgg tccagtaaag aaccacacaa cccgtaaatt aacctgcatt ttcgtatgta
     7741 atgtcttaaa gcttgataat ccattaattt tatctgaata aataaagcat gagactttgc
     7801 caggttataa tcggagaatt ccagtgaaaa tcggcccttt aactgttttg tgtgtaaatg
     7861 ttttcagtat taccatcgtt tcgggacccg aaagggctag aaaaggaggt tgggtatatg
     7921 aaaaagggct ttcttctatt acttctggcc atgatactcg ttagtagcct cgccattggt
     7981 caagtttatg atcgaaagga aacgctgtac gccggtggag gactgtggag tccgccgaac
     8041 aactggaatc cattcactcc ctgggctata atgaccggta ccaacggtct gatctacgaa
     8101 tttctgttca tgttcgatcc tctcagcaac gaaatgattc cctggctcgc tgtcgatgga
     8161 gggtggatcg atggaaagac ttacgaatta aaacttcgag acaacgtctt ctggacagac
     8221 ggagagaaat tcgacgctga agacgtgaaa tttaccttcg acatcgctaa gaaatatccg
     8281 ggtgtgcatt atagctccat gtggaactgg atgaaagaag tggagataat cgatacgctc
     8341 actgcgcgcg ttcacttcac cgaaccactc tatcagcagt ggtccttcca gctttatcag
     8401 ttgccgatgg taccggaaca catctggaag aacaagaccg aagcagaaat tttgaccggc
     8461 gccaacgaag ggccgatagg aacaggctgt tatcttgccg aaggctatgg tcaggacagg
     8521 atgatctacc tcagaaacga aaactggtgg ggaatcgagc aactgggaat caagcctacg
     8581 cccaagagaa tcgtctatct cacagtttcg ggaaacaacg tggctctggg tatgatattc
     8641 aagggagagc tcgatataag caacttcttc ctgccgggag ttcccgcagt taaagccgct
     8701 tacggaatcc acacatactt cgatggtgcg ccttacatgc tttcagataa cacggcggtt
     8761 ctcttcctaa acaacagcag aaaaccaatg gacgatgtta acttcagaaa ggctgttgct
     8821 tgggcaataa actcggacga catcgttacc agggttttcg aaaaccaggt aatcagatcc
     8881 aacccactgg gtttccttcc aatcgatgcc tggatgaagt actacgatga aaaggtagtt
     8941 gagcagtatg gcttcaaata cgatccggtt gtttcgaaga aaacactggc cgatgccggt
     9001 tacaaagata tcaacggaga tggtttcgtc gaagctccag acggttccaa ggttgaactc
     9061 tcgataattg tgcctttcgg ctggacagac tggatggagt caataaagat tatagccaac
     9121 aatctcaaaa ccgttggaat caacgctaag gcagaatttc cagactactc gaggtaccag
     9181 gacgaacttt acagcggtag attcgacatg gcgatcaaca acttcaatag caacctgtcc
     9241 aatacggtgt ggagctacta ctattggcta ttctgggata tcagagaaca acagactcag
     9301 ggaaactatg gaaaatacaa caatccaaaa gcctttgagt tgatgggtgc tttcgatcga
     9361 actccagtag atgactatga ggccggtcag aagatcatgt tggaactcga agagcttttc
     9421 ctgaaagaga ttccttacgt tcctctctgg ttcaatggta tgtggttcca ggcgagcacc
     9481 agtgtatgga gtaactggcc gagcaaacac ggtccacact actatccatg tacctggaac
     9541 ggaaagtggc agctcggtgg gatattcatg ttgtcggctc tgaaggccga ataggataca
     9601 ccggtccggt ccccgcagag aagggaatct gcgggggctt ttctgtagtt ctcttaagtt
     9661 aggggaagga gactcagact gtgggagcct atttcagaag aaaaatagtc atctatttgc
     9721 tcacgttcat ctttgcagtg acaatagact cgatgatacc gagatttatg cccggtaacc
     9781 cgattcaaaa ccttctctcg aggttttcga cccgctcgga taccaccgag gtgatcgcca
     9841 ggtatctcaa tgagatctac ggactcgaca aatcgccgtt gcaacagtat ttcggattct
     9901 gggcaggact cttcaggggc gatttgggag tgagcatata tgtcaccggg gctccggttg
     9961 ccagggtgat agcgagggcg ctgcccttcg atctggcctt gctgatcccg gcgatcatgc
    10021 tgagttacat agcaggcaat aagttcggcg ccttcgctgc aagaaaaaag aggctcgacg
    10081 gcattgtctt gcccgtctgg tacgtgctga ctgcaacacc ttatatgtgg ctgggtatac
    10141 tcctcgcatg gtttttcggg gttgcactgg atatacttcc gatagcaggg gcttacagct
    10201 tctcgatggt cccaaactgg agctggggct tcatctggga ttttttcaag cactggatcc
    10261 tccccttcag ctctctcttt attgtacagt tcggaggttg ggctattggg atgcgcaaca
    10321 tgattattta cgaactggaa gccgaatatt gcagatacct cgaatcactt ggagcctcgc
    10381 gaaaactgat aaggcgttac gcttataaaa acgcagtgct ccctcaaata acagggctgg
    10441 cccttcaatt gggtgtcata gttgccggcg cgctcgccac cgaggtggtt ttctcctatc
    10501 ccg
//
LOCUS       DAOOLY010000064        10484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7205_length_10484_cov_5.181225, whole genome shotgun sequence.
ACCESSION   DAOOLY010000064 DAOOLY010000000
VERSION     DAOOLY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7205_length_10484_cov_5.181225"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1282)
                     /locus_tag="PLD15_04730"
     CDS             complement(92..1282)
                     /locus_tag="PLD15_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63241.1"
                     /translation="MEKLTPHTLRKAQMERELQRLLGVETEIKSDSYLLSFYFPLENI
                     GRIDAEKHMRSLILEAMRSDEKLAGLGKLHQKIAEAVVLKMGNTADLGVGLAVFLQFS
                     AGESSREQNIGEVNLLALSRRPEREIYVGKTFDLDQLIWLTNSTVEALVLSLRREEAA
                     LYMLDRERLFEIAVLENEFIQEREKEYLEGYSPSPSAGTVFHGSDSEKIERLKDEENR
                     RFLQHVASVLKEEERTPRDADFIIVFYSSSFGEFVERIEDEVRLTLPAAHRVLIQKNI
                     TQEKQLLSESVKIVREEKPEIARGLMKKARENHDRYAQDWNDVARMANDRRIDTLFIN
                     PLVRKRGYILDKELVYTRAVKDSREVRNIGPWIVRSVTASNGKIVVVNGEVLESGIAA
                     RLRY"
     gene            complement(1397..2428)
                     /locus_tag="PLD15_04735"
     CDS             complement(1397..2428)
                     /locus_tag="PLD15_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63242.1"
                     /translation="MTAYILRRLISVPLTVIGVTLIVFCVIWSLGPNVLLNAYMTGST
                     GRAPNAAERIIEKYGLDEPAITRYFIWISNTLRGDLGYSMAGRMDVSEAILQMLPSSL
                     ELIFLSLIPIVLIGSRLGLRAAMNPNGPVDSVFGFLSTVGWATPDFILGLLILTGGFV
                     FFKWLPIGGLKLVAIKDWKDYTYSTLIDSILNGRIGIFFDQLVFLLEPVLTLFLVNSA
                     YIYQISRAGALEVKHADYIRTARSKGLSEREIMLKHVRKNAMIPVLTVGGELFASMVT
                     GVVFVETIFARTGIGSLIADAALMFDVLTLSGCILVISIFMITVNLLVDIAYSLVNPK
                     VTLWKELPG"
     gene            2500..3768
                     /locus_tag="PLD15_04740"
     CDS             2500..3768
                     /locus_tag="PLD15_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63243.1"
                     /translation="MANGLQGVLLGRIGKAFPAFKVKNYRYFWIGQLISVMGTWMQTA
                     AQSWLVLTITDSPFLLGLLGVAQFTPVLAFSLLAGVFVDRFPKRSILLITQFASMLLA
                     FALAVLVFTNTVQYWHIFLLSFGLGVVKALDIPARQSFMIELVGKDVLLNAIALNSTV
                     FNLARIVGPAVAGILMAMVGAGWCFLINGITFIAVILGIFKIDVRPVINKRPRGTVLP
                     DIKEGLAYLVKSPILTTTTLMLAIVSTFGMNYSVLIPVFARNQLGLDSQGYGWLMASL
                     GVGSLIGALIIAGKSAKGPSRARLFVVPFITSALFLLIGFNRSYLLSAFLICLMGLAN
                     IFFTTSANSLMQINSSNELRGRVMSVYSLVFAGSTPLGNLFVGTVSDRYGGASAFVWS
                     GAITFTLVSTVVIVHSLRKRIKRLSASGQV"
     gene            3825..4778
                     /locus_tag="PLD15_04745"
     CDS             3825..4778
                     /locus_tag="PLD15_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPB63244.1"
                     /translation="MPKIPYARISRFWGFKDKIKLVSGLNGRAYIVSSGRSLYVLKRR
                     PIDVAGFAVQQVFLSSLFQNGINVSLPLENPDGLFEVKIGKFVYSLFTYIEGNKADLD
                     ISCATPEMAREIGLFTGRLHSALSRLNDNTIQRRNLFTTLSSSLRFLSCQGDRLPYLD
                     RERIEEFFGEFFSLYYRLPVQAIHGDYHPGNIIVNSGRVSGVIDFEYSCREVKVLDLS
                     YLISSVLTEFKRMGSPSLALYLIPHLLEGYCLKNTLTIEERESIVYLLGAVLLNQVHF
                     FLARGCTEEAAFAESILEWVVDNRERINDKAGLTYTHSTSR"
     gene            complement(4825..4965)
                     /locus_tag="PLD15_04750"
     CDS             complement(4825..4965)
                     /locus_tag="PLD15_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63245.1"
                     /translation="MIHNHYPTIEDLKTGEIEIERELESLRNERIWRKIRRSLRDAKK
                     RR"
     gene            5200..6699
                     /locus_tag="PLD15_04755"
     CDS             5200..6699
                     /locus_tag="PLD15_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPB63246.1"
                     /translation="MIGLKVSIERNTNVPLFKQLTEQLRAAILRRDIPEGTRLPSERE
                     MARLLKLNRSVVIKAYNELKLDALLDSKSGSGTYVAAVTPLKKEFGMISWSDQLSTWA
                     TSSSGEARWSSLAKGRPADCIRLDTGVPEVRTDQMTLLKKILEGLTQEELDESLQYPS
                     LRGNPELLKYLSIRLNGSGMKVSAKNLLVTIGTEEAIYLLFSALARPGEAVIIENPTY
                     LNVIKISRMFQHRVLPVNRFSEGFDMATLENTLQRGSVKYIYTMSCSHNPTGANMPER
                     KKESLVRLAEKYSVPVIDDTVFSEIQYQSPMPRTLKYYDTHNCVIEIGGISKSYAPGL
                     RIGWIVADEALIEKLIEPMRMISLGVPNLSQLVAAKLLSSGEYDAYLERYIKLSLRKC
                     EKTRMACYRYLPQYVKVNEAQGGNFFWLELPDSMDCWKVAEESLKEGVAVSPGSLFTF
                     DSSGKNYLRLNPLGVPEEEIEKGIERLSKAIATVASHYRKEEQSLSVIV"
     gene            complement(6689..7720)
                     /locus_tag="PLD15_04760"
     CDS             complement(6689..7720)
                     /locus_tag="PLD15_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB63247.1"
                     /translation="MKRYNLHAVNAEKLPDPPFLLVGNHAYFIDAALIEAFVKYPIVW
                     AVAAGNFKLPLAASILKAAGAIEKRKGVPDVAAMRKILRVLKDGGIIGLMPEGSVTWD
                     GEFGEVPPGTEKFLDRVDVPVVAARMNGGYLTRPRWADHHRWGRIEIVFEVFRGSEAL
                     DFLSRSSDWEWQREKRILFKGRKRASGIERIVWFCEKCGGFRTIAARGDEAVCAECGA
                     ALIVDEYGYISGKSVPEVLKVQRELLAAYLSREGSLKVGPGEAVILNTLTEGIVKVRG
                     EVSIDGLELKVGERSYRLENARGFSTYLKRLNEFNYDRDVIRLKTEHSSYLLYCAYRI
                     LAGPEAATL"
     gene            7986..9881
                     /locus_tag="PLD15_04765"
     CDS             7986..9881
                     /locus_tag="PLD15_04765"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HPB63248.1"
                     /translation="MRDDQRNEVSKQLKAGKDNLSPGVEIRKKSPSKPKGETFNLFDY
                     NIEAPPESESKVSNSKRQELVDARYIYPNTEQALDELANLVDSTVLAIDTETTGLDPI
                     TDRVRLVQLGVPGKPVLLLDMDTITSVSGPLNELLGSEKTLKLFHNAKFDIEFLKIAG
                     IDVKGPLFDTMLAELLLTSGLAGTSAKLSDMSKKYLNVELPKEQQLSDWTGTLTQEQL
                     EYAARDVAVLLELYTPLSIAIEESGLSRVAELEFEALPAVVALELNGIFLDTRRLEKL
                     TAVLQSELEESVMECRELLDAGINLDSPKQLLRALSAKGIRVDNTSASTLEKLRNRHP
                     EVAAILRYRRAAKAISTFSNKLTDHIHPVSGRIHPSYWQIGASTGRFSCSNPNLQQIP
                     KEARFRDCFVPAPGNKFVIADYSQIELRVAAAISRDPVMIKAYKNNEDLHRETAAVLI
                     GKKPSEITKEERQLAKAVNFGLLFAMGARGLSNYAESGYGVKMSVKEAQEFIERFFNH
                     YTGLKYLKRRVGLSSQHEVRTLSGRRYLLSEDTGLSVKLNIPVQGTAADLLKIALGRL
                     PRALEDTGAMIVAIVHDEIILEVPESAAEKAKRILVEVMEGAGDGLMDVPLVAEATIA
                     DSWAGKA"
BASE COUNT         2535 a         2797 c         2663 g         2489 t
ORIGIN      
        1 aaacacgagc gaaaataggg actgatgggc tcctgacgtc tgtcccattt ttcgcgtgtc
       61 tccctttctc tctctctcct cttcccgctc ttcagtatct caatctggcc gcgataccgc
      121 tctcgaggac ttccccgttc acgaccacta ttttaccgtt gctggccgtt acgctccgca
      181 ctatccacgg gcctatgttc ctaacctctc tgctgtcttt cacggctctc gtgtagacga
      241 gctctttatc gagaatatac cctctcttcc ttacgagcgg gtttatgaac aatgtatcta
      301 ttctcctgtc gttggccatt ctggcgacgt cgttccagtc ttgagcgtat cgatcgtggt
      361 tctctctggc ctttttcata agccccctgg caatctccgg cttttcttct ctgactatct
      421 tcacgctttc ggaaaggagt tgcttctcct gggtgatgtt tttctggatc aagacgcggt
      481 gggccgcggg gagcgttagt ctcacttcat cttcgatcct ctctacgaat tcgccgaaag
      541 aagaggaata gaagacgatt atgaagtcgg cgtcgcgcgg cgtcctttcc tcctctttca
      601 aaacgctggc gacgtgctgg agaaatctgc ggttctcctc gtctttcaac ctctcgatct
      661 tctccgagtc gctcccgtgg aaaaccgttc ccgccgatgg tgagggcgaa taaccttcta
      721 gatactcctt ttccctctcc tgaatgaact cgttttccag tacggcgatc tcaaaaagtc
      781 tctctctatc gagcatatac agggccgcct cttctctcct gagacttagg accagcgctt
      841 cgaccgtgga attggtgagc catatgagct ggtcgagatc gaaagttttg cccacgtata
      901 tctccctttc gggtcttctc gatagggcga gcaaattcac ctctccaatg ttctgttctc
      961 tggagctttc accggcgctg aactgcaaga aaacggccag gcccacaccg aggtcggctg
     1021 tgtttcccat tttcaatacg acggcttcgg cgatcttctg gtgaagcttt ccaagtccgg
     1081 ccagtttttc gtccgagcgc atcgcttcta gaatcaaaga tctcatgtgt ttttcagcgt
     1141 cgattctgcc gatgttttcc agcgggaagt aaaaactgag aagataagaa tcggatttta
     1201 tttctgtttc aacgcctaga agtctctgta gttccctttc catctgagct tttcgaagag
     1261 tatgtggagt gagtttttcc atcgtatttc ctccttctgt gcccggtggt tgtgagtact
     1321 tcgatcgttg accgctttag attctatgct tttttgatcg gatggagacg atatatgttc
     1381 aaatgttccg aataacttat ccgggaagct ctttccagag cgttacttta gggttcacca
     1441 gtgagtaggc tatatccacc agcaggttca ccgtgatcat aaatatagag atcaccagga
     1501 tacaaccgga caacgtcaag acgtcgaaca taagggctgc gtcggcgatc aagctcccta
     1561 tgcccgttct ggcgaatata gtttcgacga atacgacccc ggtcaccatg gaggcgaaaa
     1621 gttcgccgcc gaccgtgagt acggggatca tagcgttttt tctcacgtgt ttgagcatta
     1681 tctctctctc gctcaagcct ttcgagcggg ccgtccgtat gtaatcggcg tgcttgactt
     1741 cgagcgcgcc ggccctgctg atctggtata tgtaggccga gttcaccaga aagagggtca
     1801 ataccggctc gaggagaaag accagctggt cgaaaaagat cccgatacgg ccgttcagga
     1861 tagagtcgat cagcgtcgaa taggtataat ccttccagtc tttgatcgcg accagtttaa
     1921 ggccgccgat cggaagccat ttgaaaaaga cgaatccccc tgtcaggatt aagagcccga
     1981 gtatgaagtc gggcgtcgcc cagccgacgg ttgaaagaaa gccgaagact gaatccaccg
     2041 ggccattggg attcatggcg gctctgagac ccaggcgaga acctatcaga acgatgggaa
     2101 tcagcgaaag aaagatcagc tctagactac tcggcaacat ctggagaatg gcctcgctaa
     2161 catccattct tccggccata gaatacccca gatcgccccg gagcgtattc gaaatccaga
     2221 taaagtacct ggttatggcc ggctcgtcta agccgtactt ttcaataatc ctctccgcag
     2281 cgttgggagc cctgccggtg ctgcccgtca tataggcgtt caaaagaacg ttcgggccga
     2341 gcgaccatat aacacagaat acgatcaggg tgactccgat caccgttaga ggaaccgaga
     2401 taagccttct taggatgtaa gcggtcatgt aaaacctcct gaacgtacat ctgattatag
     2461 aacaaaaaat gtagtacaat tgtactggag atgattcgtt tggctaacgg tttgcagggt
     2521 gtactcctcg gtcgtatcgg caaagccttt ccggctttta aagtgaagaa ttaccggtat
     2581 ttctggatcg gtcaactcat ttcggtcatg ggcacgtgga tgcagaccgc ggcccagtcg
     2641 tggctcgtgt tgaccataac ggactcgccc tttcttctgg gactcctggg agttgcccag
     2701 ttcacgccgg tactcgcctt ctcgctactg gcaggcgttt tcgtcgatcg cttcccgaag
     2761 agatctattc tgctgatcac ccagttcgcc tcaatgctgc tggccttcgc gctggccgtt
     2821 ctggttttca cgaacactgt gcagtactgg catatctttc tgctctcctt cggcctgggt
     2881 gtggtgaaag ctctggatat accggccagg caatccttca tgatcgagct ggtaggcaaa
     2941 gacgtgcttc tcaacgccat cgccctcaat tcaactgttt tcaacctcgc caggatagtc
     3001 ggcccggcag tcgccgggat attgatggcg atggtcggag ccggctggtg ctttctcata
     3061 aacggcataa cttttatagc ggtcatactc ggcatcttca agatagacgt gaggcctgtt
     3121 ataaacaaga gaccacgcgg aacggttctg ccggatatca aggaggggct tgcatatctc
     3181 gtgaagagcc caatactgac caccacgaca ctgatgctgg cgatagtgag caccttcggg
     3241 atgaattaca gcgtgctcat accggtcttc gcgcgcaacc agctcgggct ggactctcag
     3301 gggtacggct ggctcatggc ctcactcgga gtgggctcgc tgatcggtgc gcttataatc
     3361 gccggaaaga gcgcgaaagg ccccagccgc gcgcgactct tcgtggtccc cttcatcacc
     3421 tcggcgctct ttctgctgat cggcttcaac aggtcgtatc tcctctccgc cttccttata
     3481 tgcctgatgg gcctggccaa tatcttcttc acaacttctg ccaacagcct gatgcaaata
     3541 aactccagca acgaactgag aggcagagtc atgagcgtct attcgctcgt cttcgccggc
     3601 tcgacgccgc tgggtaatct cttcgtagga acggtctccg acaggtacgg aggggcgagc
     3661 gccttcgtct ggtcgggcgc gatcaccttc acgcttgtct ccacggtagt tatcgtacac
     3721 agcctcagaa aaaggatcaa acgcttgagt gcgagcgggc aggtttgagt cattttctcc
     3781 tgaatctgta gtataattct tttattgtat tccggggtga taaaatgcca aagattccct
     3841 atgcccggat ttccaggttc tgggggttca aagacaagat caagctcgtt tccgggctca
     3901 atggtcgtgc atatatagtt agttcgggcc ggtctcttta cgtcctcaag aggcggccga
     3961 tcgatgttgc gggttttgcc gtgcaacagg tttttctctc ctctctgttt caaaatggga
     4021 taaacgtctc gctcccgctc gagaatcccg acgggctttt cgaggtcaag atcggaaagt
     4081 tcgtttactc tctcttcacg tacatagagg gaaacaaggc agatctggat atatcctgcg
     4141 ccactccgga aatggcccgc gagatcggcc tcttcaccgg acggctgcac tcggcgctct
     4201 ccagactgaa cgacaacacc atccagagaa ggaatctctt cacgacgtta tcgagctcgc
     4261 tgcgttttct ctcctgccag ggagaccgtc tgccgtattt agaccgcgag aggatcgaag
     4321 agtttttcgg cgagttcttc tcgctctact acaggcttcc ggttcaggcg atacacgggg
     4381 attaccaccc gggcaatata atcgtcaatt ccggaagggt ttccggggtc atagacttcg
     4441 aatactcctg cagggaagtc aaggttttag atctctccta tctcatatcc agcgtgttga
     4501 ccgagttcaa gagaatgggc tctccctcgc tggcgctcta cctgatccct caccttctgg
     4561 aggggtactg cctaaagaac acgttgacga ttgaagaaag ggagagcatc gtgtatcttc
     4621 tgggggccgt tctcctgaac caggttcatt tctttctcgc ccgcggctgt accgaggagg
     4681 ccgccttcgc agaatcgatt ctggaatggg tcgtggacaa cagggagaga ataaacgaca
     4741 aggctggcct gacttacact cattccactt ccagatgagg cgactcagcc gattctgaag
     4801 actccgccgt gactcctgac aactttaccg tctttttttc gcatctctga ggctccttct
     4861 gattttcctc cagatccttt catttcgcag actctccagt tccctctcga tttcgatctc
     4921 cccggtcttg agatcctcta tagtcggata gtggttgtgt atcatctacg atccctccct
     4981 tcgctatgaa tatatcaccg cccaggaacg gctgtctttc caccggcaaa ggattggaac
     5041 gatccatttc ctctgccatc ctattcagca tcaattgctg aaagtctctt tgtataagca
     5101 ttaaatagac aagagataag tggtacgctc tgctggcctg tcagtggtat ggtagagcgg
     5161 accgcaaaag ttataatcgt ttcgtagggg gtgaggccgg tgataggtct gaaagtatcg
     5221 atcgagagaa atacgaacgt acctcttttc aaacagctca ccgagcagtt gagggctgcg
     5281 attttaaggc gggacatacc ggaaggaacg cgactgccct ccgagcgcga gatggccaga
     5341 ctccttaaac tcaacaggag cgtggtgatc aaagcctaca acgagctgaa gctggacgct
     5401 ctcctcgatt cgaagtccgg cagcggcact tatgtggccg cggtgacacc gctcaagaaa
     5461 gagttcggca tgatatcctg gagcgatcag ctcagcacct gggccacatc ttcctcggga
     5521 gaggcccgct ggtcatcgct cgcaaagggt cggccggccg actgcatcag gctcgacacg
     5581 ggagttcccg aggtgcggac cgaccagatg actcttctga aaaagatact ggaaggtcta
     5641 acacaggaag aactggacga atcgctccag tacccctcgc tgaggggcaa ccccgagctc
     5701 ctcaagtatc tctccataag gctgaacggc tcgggtatga aagtctccgc gaaaaacctt
     5761 ctcgtaacga tcggaaccga ggaggcgatc tacctgttgt tttccgctct ggccagaccg
     5821 ggagaggccg tcataatcga gaaccccaca tatttgaacg ttataaaaat ctccaggatg
     5881 ttccagcacc gggtcctgcc tgtaaacagg ttcagtgagg gcttcgacat ggccacgctg
     5941 gaaaacactc tccagagggg ctcggtgaag tacatataca ccatgagctg ctcccacaac
     6001 cctaccgggg cgaacatgcc cgaacggaaa aaggaatcgc tcgtcaggct tgccgaaaag
     6061 tattccgtgc ctgtaatcga cgatacggtc ttttcggaaa tccagtacca gagccctatg
     6121 cccagaacct tgaaatacta cgacactcac aactgcgtta tcgagatcgg cggtatttca
     6181 aagtcatacg cgccgggttt gaggatcggc tggatcgtgg ccgacgaggc gctcatagaa
     6241 aagctgatcg agccgatgag aatgatctcg ctgggtgtgc cgaacctgag ccagctggtg
     6301 gccgcgaagc tcctctcctc gggtgagtac gacgcttacc tggagcgcta catcaaacta
     6361 tcgctcagga aatgcgaaaa gacccggatg gcctgctacc ggtacctgcc gcagtacgtg
     6421 aaggtgaacg aagcgcaggg cgggaacttc ttctggctgg agctccccga ttccatggat
     6481 tgctggaaag tcgccgaaga atccctgaaa gagggcgtcg cagtctcgcc gggcagcctc
     6541 ttcactttcg attcctccgg aaagaactac ttgagactga accctctcgg ggtaccggaa
     6601 gaagaaatcg aaaaaggtat agaaagactc tcgaaggcca tcgcgacggt agcgtctcac
     6661 tacaggaagg aggagcagag cctctcggtt atagtgtagc agcctccggg cctgcaagaa
     6721 tgcgataggc gcagtagaga agataagagg agtgttccgt cttcagtctt atcacatcgc
     6781 ggtcgtagtt gaactcgttc aaacgcttca gataagtgga gaagcccctg gcgttttcca
     6841 gccgatagct cctctctccg accttaagct cgaggccgtc gatcgaaacc tcgcctctga
     6901 ctttcactat accctccgtg agagtgttca aaatcacggc ctctcccggg ccgaccttga
     6961 gggaaccctc tctcgataga tatgccgcca gaagctctct ctggaccttc aaaacctcag
     7021 gcacgctctt tcctgagata tagccatact catcgactat cagagcggca ccgcattcag
     7081 cgcagacggc ctcgtcgcct ctggcggcga ttgtgcgaaa gcccccgcat ttctcgcaga
     7141 accatacgat cctctctatg ccggaggccc ttttccgccc tttgaaaagt atcctcttct
     7201 ctctctgcca ttcccagtcg gaggaacgcg aaagaaaatc cagcgcctcg cttccgcgaa
     7261 agacctcgaa aacgatctcg atccttcccc agcggtggtg atcggcccac ctcggcctgg
     7321 taagataacc cccgttcatc ctcgccgcca ccacgggaac atcgaccctg tcgagaaact
     7381 tctcagtgcc gggtggcacc tcgccgaact cgccgtccca ggttacagag ccctcgggca
     7441 tcagaccgat tatgcctccg tccttcaaca ccctgaggat ctttctcatc gcggcgacat
     7501 ccgggactcc cttcctcttt tcgatcgcgc ccgccgcctt caaaatagag gctgccaggg
     7561 gaagcttgaa attgccggcc gcgacggccc acacgatggg atacttcaca aaagcctcta
     7621 tcagagcggc atctatgaaa taagcgtggt ttccgaccag aaggaagggc ggatcgggaa
     7681 gcttttcggc gttcaccgcg tgaagattgt agcgtttcat gaaggggcgc ctcagaatag
     7741 gccgcaaagc tcgccagata aacctgtcgc ccggcagtct ctctctccgc aaatccgtca
     7801 cttccccaag agtataagta tagtagatta tacagtggca aaaggactga aagctaatcg
     7861 aactgtagac cgcatgtata atgttaatta atcggagtaa cggtacctga atgagccggc
     7921 acaaaaaaga cgatccggtc gactatgcaa tgcactggaa ttgagcttca aatgggggta
     7981 agcttatgag agacgatcaa cggaatgagg tgtcaaagca attgaaagcg ggaaaagaca
     8041 acttatcccc cggagtggaa attagaaaga agagcccatc gaaaccaaaa ggcgagactt
     8101 tcaatctatt tgattacaat atagaggcgc ccccggaaag cgaaagcaag gtgagtaatt
     8161 caaagcgtca ggaactagtg gatgcgagat atatataccc gaataccgaa caggctctcg
     8221 atgagctcgc caatttagta gattccacag tgttagcgat agataccgag acgacgggat
     8281 tggatcccat aaccgaccgc gtaagactcg tgcagcttgg cgttcccgga aagcccgttc
     8341 tcctccttga tatggatacg ataacgtctg tctccggccc tttaaatgaa ttactgggca
     8401 gtgaaaagac tctgaagctc ttccacaacg ccaagttcga tatcgagttc cttaaaatcg
     8461 ccggaataga cgtgaaggga cctctattcg acactatgct tgccgagttg ctcctgacct
     8521 ccgggcttgc cggcacatcc gccaaactgt ctgacatgtc gaagaagtac ttgaacgtgg
     8581 agctgcccaa ggaacaacag ctgagcgact ggaccggaac actgacacag gaacagctcg
     8641 agtacgccgc cagggatgtt gcagtactgc tggagctcta cacaccgtta tcgatagcga
     8701 tagaggaatc aggactgtcc agagtggcgg agctcgaatt tgaagcactt ccggcagtgg
     8761 tcgcccttga actgaacggg atttttctcg acactcgcag actggaaaaa ttgaccgcag
     8821 ttcttcagag tgaacttgaa gaatcggtta tggaatgcag agagcttctc gatgccggta
     8881 tcaatctcga cagccccaaa caattgctga gagcattgag cgctaaaggt ataagagtcg
     8941 ataacaccag cgcctctaca ctcgagaagc tgagaaacag gcatcccgaa gtggctgcga
     9001 tattgcgcta tcgcagggcc gcaaaggcca tctcgacttt ctcgaataaa ttgacagacc
     9061 acattcaccc tgtcagcgga agaatccatc catcctactg gcaaatcgga gcctctacgg
     9121 gtcgtttttc ctgctcgaac ccgaaccttc aacagattcc caaagaggcc agatttcgcg
     9181 attgcttcgt accggcgccg ggaaacaagt tcgttatcgc ggactattca cagatcgaat
     9241 tgagggtggc ggcagctata agtcgagacc ctgtaatgat caaggcttac aaaaacaacg
     9301 aggatctcca ccgggaaacc gcggccgtgc tcatcggaaa gaaaccctcg gaaataacga
     9361 aagaggagcg ccagctagca aaagccgtga atttcggcct gctttttgca atgggagcga
     9421 ggggactttc gaattacgct gaaagcggtt atggtgtgaa aatgagcgtg aaagaagcac
     9481 aggagttcat tgagcgattc ttcaaccact acaccggatt gaaatatctg aaacgaagag
     9541 taggtttgag ttcacaacac gaagtcagaa ctctcagcgg cagaagatat ttattatcgg
     9601 aagacaccgg tctttctgtg aaattgaata ttcctgtgca aggcacggcg gcagatttgc
     9661 tgaaaatagc cctcggcaga ctccctcgag ctcttgaaga cacgggggcc atgatagtgg
     9721 cgatcgttca tgacgagatc attctggaag ttcccgaaag tgccgctgaa aaagcgaaga
     9781 ggatactggt ggaagttatg gaaggcgccg gtgatggtct catggacgtg cctctcgtgg
     9841 cagaagcaac catagcagac agctgggccg gaaaagctta actgactttg acagcaaaag
     9901 agaatcggtg gtatagaaag cgcataacat ttaggactaa cagatccatt tggaaagagc
     9961 cgtccttcgg aagagcgaga ggaggagaga gaagagagag gagagactga agagagagga
    10021 tcccgtatag gagcattacg ggatgacagg cttcctccct tcccgtcatg ccggacctcg
    10081 atccggcatc ccgctcttac ccacaacaca ccacgcacaa ccaacaacgt ttcttaagag
    10141 aattctcgtc ttctcgtgag cgaagcgaac tcttgacgct ctttctctcg tgtttttacg
    10201 aacaaccaac aaccattctt attaggaact ttcgggatga caatttcaca ggtgcgtgcg
    10261 aatgcatgtc aacggctatg gatataggtt tcaaattctc ccctccttac tctaattcga
    10321 agcgcataaa ttgagaagaa gagtttagat cactatacga attgataaat taacctaact
    10381 aatagtaaaa taggagagcc agcgttctta aaaacctttt tagtgaagtc ttctttggta
    10441 gtctctgctg aaagggaaag ggtgaacggc atgctaacgt tgat
//
LOCUS       DAOOLY010000065        10339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7384_length_10339_cov_4.726663, whole genome shotgun sequence.
ACCESSION   DAOOLY010000065 DAOOLY010000000
VERSION     DAOOLY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_10339_cov_4.726663"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLD15_04770"
     CDS             complement(<1..875)
                     /locus_tag="PLD15_04770"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HPB63249.1"
                     /translation="MLAALMGIMIAVLYIPWRFRFDRGYVKTALVTESTVEGVDSLRL
                     IPANELKSRPSEIGISVIFVDLKDHWSLLPEILPLQRPIVLTGISPYTAVQLAEMLDS
                     HSAYTGYMEFDERGEYVKEVLKLRREKSLVFRVHNLKKKEYPNYDVQAAVVRYIRAVR
                     ERSIDMLLFLKPENDFAYEDFVRQVDDKLGELNLLQSSVTSPRAGNSRFFILSTAFIF
                     FLVASLSPFLALVVLPLWIFFPSIGLPLGAISGHFAIYYYLSRTVRDTVRGILLFFVF
                     SLFLGLSINSSMLSPA"
     gene            complement(899..1678)
                     /locus_tag="PLD15_04775"
     CDS             complement(899..1678)
                     /locus_tag="PLD15_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63250.1"
                     /translation="MRVRSLLILWLLVSISLFAQVETKIRNEIRAANDELIEIYGFQV
                     RYNLTILTGQGHEQPAAIDDNGMYQIFLYTSNFRDGVARHELAHVYFFEYLRKIGSSP
                     ETIPLWYHELIAEGFQNLRSRSGRPPLRSGFFDFTVSIKNYPPEKDRSVFYGAVESFA
                     GFLLSRFDYENLLHLVDEFSSSGDMNSSFKAIFGSSLDNLIIKWRVLFLLPYSPFLVG
                     VVIFLYLLIGRRERYWRQFPLDPLNPESLKRGESGEIDQKS"
     gene            complement(1675..2544)
                     /locus_tag="PLD15_04780"
     CDS             complement(1675..2544)
                     /locus_tag="PLD15_04780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPB63251.1"
                     /translation="MDNGNYYERLDRFLRNVLNDLKLSSIYKLLRKGNVKVNGKRVKD
                     ESHRLSLGDVLQVYYLGDSSKMKRLEETRELVPQKIPLDILYEDDTTIAIDKPAGISV
                     HPGKGIQIITLIEGLLAYGREKGFEPHPVHRLDKHTSGVILFAKSPASAREISRLFRE
                     HEVEKIYLTLVKGCPQIESARLESLNEGILEVLEYSLEKRYREVSLLKVRLHTGKKHQ
                     IRRQMAQINCPVAGDDVYGDRTFNRNFKKASGLKRYFLHCQHLGFTCSSGLKIAVDSE
                     LPEDLLKVLASIE"
     gene            complement(2610..2861)
                     /gene="acpP"
                     /locus_tag="PLD15_04785"
     CDS             complement(2610..2861)
                     /gene="acpP"
                     /locus_tag="PLD15_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB63252.1"
                     /translation="MTHDEVFDRVKTIIAEKLGVEEDEIALDSDLTEDLGADSLDLVD
                     LVMAFEDEFNFKVEDEQIENISTVGDIVEQISKGLKVEG"
     gene            3007..3834
                     /locus_tag="PLD15_04790"
     CDS             3007..3834
                     /locus_tag="PLD15_04790"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPB63253.1"
                     /translation="MFNYFEAASSMIKEVGVKPEVALILGSGLGYLTGEFQGGVSVDY
                     SKIQGFPVSTVKGHSGRFVFGSFHDIPSVAMEGRFHFYEGHEIKDVVSPIYLFKEMGI
                     ERLLITNASGGINRTYSPGDIVAIKDIINLGFRNPLIGPNDDRFGVRFPDMSSVMDFK
                     WLSGLRERLSRDGRELKEGTYIWALGPSYESPSEIRSFEFLGADMVGMSTVPEVIAAN
                     HCRMKILALSCITNMASGILKEKLSHDDVVGTANRIRHRFSDVVGKALEVLKEIGHE"
     gene            3827..4831
                     /locus_tag="PLD15_04795"
     CDS             3827..4831
                     /locus_tag="PLD15_04795"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPB63254.1"
                     /translation="MNRTMNAVFSKLLQAQNILVCGHIMPDGDCVSSVVSLSMGLEKL
                     GKKTTMAIDWKIPSIFSPFPRVERIIDYSRYSTQLENSDLLVIVDASSPDRIGRFERL
                     LRYGMPSILIDHHATNTRFANLCWVEPSYSSTAQMIFDLLKLMEVSYDGDLALMNYLG
                     IATDTGFFRYSNTNSTVFEAAAELVKLGADPCLVATAILETRRLEEFFLERDAIDNLR
                     LLSNDRFAYSYLRVEDFERYSLGEDDFGGFVGDLRSIASVEVALFASESVGGEAHVSL
                     RSKSYFDVSSVAVAFGGGGHPRAAGFTLKYDGDLEEALQEAVELISRRLESHSKDATL
                     "
     gene            complement(4821..5447)
                     /locus_tag="PLD15_04800"
     CDS             complement(4821..5447)
                     /locus_tag="PLD15_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB63255.1"
                     /translation="MLRNFISLTPAIIVAILTHEYSHYYLARYFDRTRTEWSRPGFIR
                     RIDPVGLLMYYLFKFGWSRPYPVNYWKLRKLGYSKAILTALSGSLGNFVVGLLAGLIF
                     YLSGLHRYSTLMPDYLSSYPLSYFADVIYWIMFIDLSTAFFNLLPIPPLDGANIIAVI
                     VPENYVNWLVKYELYGILVLLVLSLIGVIQLVMWPVTQFIQILSWLIV"
     gene            complement(5440..6153)
                     /gene="rsmI"
                     /locus_tag="PLD15_04805"
     CDS             complement(5440..6153)
                     /gene="rsmI"
                     /locus_tag="PLD15_04805"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB63256.1"
                     /translation="MDSEYGRLYLVGTPIGNLEDITLRALRVLGEVELVIAEDTRRAS
                     NLMSALELKRREIISFNMHNQSRRIPALLARLKNGDKIALVSDAGMPVISDPGATLVR
                     ICRENCIEVDVVPGPSAVTTAVALSGFPGTNFTFVGFLPRGKNRRRLFRKISAGTFEE
                     SLIVFFESPYRLLETLRDLLEILGDRDIFVAREMTKKFQQSLNGKISEIIAHFASSEP
                     VGELTVVVSGRSGEDDRDA"
     gene            complement(6153..6821)
                     /gene="rsmG"
                     /locus_tag="PLD15_04810"
     CDS             complement(6153..6821)
                     /gene="rsmG"
                     /locus_tag="PLD15_04810"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPB63257.1"
                     /translation="MNLGESFSSDTCERFQRLVDLLISSPHNLTSVRDRERAMVAHIE
                     DSLLAFEGYKLEGSYIDIGSGGGIPGLVLAIAFPETRWVLVDSIAKKVREIERFSSTL
                     SLSNVTAVTSRIEEYPKGIDRFDGAVMRAVARSDAALELAAPLVENGGLIHLYKGPGW
                     KDEECYAFVASAKLGLQLQDERNYRLSDGSSRKLVTFKKVSETPKGFPRRPGMASKFP
                     LGEK"
     gene            complement(6818..7588)
                     /gene="folE2"
                     /locus_tag="PLD15_04815"
     CDS             complement(6818..7588)
                     /gene="folE2"
                     /locus_tag="PLD15_04815"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPB63258.1"
                     /translation="MRDVQNERDRRNIRINMVGIKSIKYPIIVMDRAFGNQHTVGTFD
                     LFVDLPKDFRGTHMSRFVEVLERHHKKITPKNMESILEDMRQALNADVAHIEVEFPYF
                     MRKKAPVSNSESFSSFVCSFISRKDGDFDFVLRVRVPVMTVCPCSKEISERGAHNQRA
                     EVTVSVRMNSLIWIEEIIDIVETSASAPTYSLLKREDEKFITEHSYDNPRFVEDLSRE
                     VVLKLNKDSRVDWYRVEVLSQESIHNHEAYACIEKGSR"
     gene            7639..7727
                     /locus_tag="PLD15_04820"
     tRNA            7639..7727
                     /locus_tag="PLD15_04820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7673..7675,aa:Ser,seq:tga)
     gene            complement(7747..8580)
                     /gene="lepB"
                     /locus_tag="PLD15_04825"
     CDS             complement(7747..8580)
                     /gene="lepB"
                     /locus_tag="PLD15_04825"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB63259.1"
                     /translation="MKSEKTSGKKGSRFLHEIKEWGKAILYAVVFGTIIRLFVFETML
                     VPTGSMIPTINPPARLFVEKITYSYREPDYGDIVVFWTPYVDIESQKYLRGFDKFMDF
                     FSPAEFKGHVKYVKRLVGKPGDILELRRAPDYTAARPVYQLYVNGEVPPKLENRRYVK
                     EGIFNDTRFYFGLAHPDDASVRFSLYYRLFQAYKGLIEYTEYYDSVLAPLGMETYIKT
                     DPETGSILVTVPEGFRFMMGDNSTESFDSRYFGFVPERAIVGSPMLTIWPLEDFGPLK
                     K"
     gene            complement(8596..8943)
                     /gene="rplS"
                     /locus_tag="PLD15_04830"
     CDS             complement(8596..8943)
                     /gene="rplS"
                     /locus_tag="PLD15_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPB63260.1"
                     /translation="MDQYIRSLENEFMRQDLPEIGPGDTVRVSVRIKEGNKERVQAFE
                     GIVMGIRGSGTGKSFTVRRVGAAGVGVERIFPFTSPSLEKVEVLRKGKVRRAKIYYIR
                     NVKGKVKIKEKRD"
     gene            complement(8946..9527)
                     /locus_tag="PLD15_04835"
     CDS             complement(8946..9527)
                     /locus_tag="PLD15_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPB63261.1"
                     /translation="MLNNIYLALMHYPVLGRHANIVSSAVTNLDIHDISRTCRTYNIK
                     GYYVVSNLPAQREIVDNVLSYWLEEFGREYNPSRSEALTVVRKASYLEDVIEDIESVE
                     GVRPHLVFTSAKRGPDRLSYGEMSGLLRTSEIPHLLLFGTSWGLPEEILKLCKFALEP
                     IRANSDFNHLSVRAAVAVILDRLIGEDTEKRRD"
     gene            complement(9517..10260)
                     /gene="trmD"
                     /locus_tag="PLD15_04840"
     CDS             complement(9517..10260)
                     /gene="trmD"
                     /locus_tag="PLD15_04840"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPB63262.1"
                     /translation="MKIDVLTIFPDLFEAVLKWGVISRAIESGLIEFGAVDLRDYTDD
                     RHRTTDDYPFGGGSGLVMKADPILRAVDTLRTDGPAPYVIYTSPQGEIFDNARARDLS
                     KKERLLFICGRYEGIDERAMSIVDEELSIGDFVLSGGEIPVLLMVEALSRFVPGVVGD
                     MESVVNDSFYRDLLDHSHYTRPRNVRGLEVPDVLLSGDHEKIEIFRRKESLERTMERR
                     PDIFLKHELDLYDKKAILSLFEELRKNVE"
BASE COUNT         2541 a         2649 c         2434 g         2715 t
ORIGIN      
        1 gccggcgata gcatagaaga gttgatcgac aatcccagga aaagcgaaaa cacaaaaaac
       61 aacaggattc ctctcacagt atctctgaca gtcctcgata gatagtaata tatggcgaag
      121 tgacccgata tcgctcccag aggcagtccg atagaaggga agaatatcca taacggcagg
      181 accaccagtg ccaggaaggg gctgagggag gctaccagaa agaaaatgaa agctgtagaa
      241 agaatgaaaa acctggaatt acccgctctg ggagaggtca ccgaactctg aagaaggttc
      301 agctctccaa gtttatcgtc cacctgtctt acaaaatcct cgtaggcgaa gtcgttttcc
      361 ggttttaaga acagcagcat gtcgatgctt ctctcgcgaa cggccctgat atatctcacg
      421 acggctgcct gaacatcgta attagggtat tcctttttct tcaggttgtg caccctgaag
      481 accagtgatt tctcccttcg caacttcaat acctctttga cgtactcgcc tcgctcatcg
      541 aactccatat atccggtgta cgccgaatga ctgtcaagca tctcggccag ttgcacggcg
      601 gtatagggag aaataccggt caatacaata ggtctctgga gggggagaat ctccggaaga
      661 agcgaccagt gatccttcag atcgacgaat ataactgaaa taccgatttc cgatggacgc
      721 gacttcaact cgttcgctgg gatcaacctc agagaatcga cgccttcgac agtgctttcg
      781 gtaaccaggg cggttttgac atatcctctg tcgaatctga atctccatgg aatgtacagt
      841 acggcgatca ttatacccat tagagcagcc agcaaaatca atcttccggt gagttgtttt
      901 acgacttttg atcgatctct ccgctttctc ctcttttcag actttctggg tttagtgggt
      961 ctaaggggaa ctgccgccaa tatctttccc tcctcccgat cagaagataa agaaatatta
     1021 caacaccgac cagaaatgga gagtaaggca atagaaatag cactctccat ttaatgatca
     1081 ggttgtccag actggatcca aaaatcgctt tgaaagacga gttcatatca ccactcgaag
     1141 agaactcatc gaccaggtgc agtaggtttt cataatcgaa cctcgaaagc agaaaaccgg
     1201 caaagctttc gaccgcaccg tagaagacag atctgtcttt ctctggcgga taattcttta
     1261 tagaaactgt gaagtcgaaa aatccagatc tgagcggggg tctccccgac cgggaacgca
     1321 gattctgaaa gccctcggca atcaactcat gataccacaa tggtatcgtt tcagggctcg
     1381 agcctatctt tcttaaatat tcgaagaaat agacatgtgc cagttcgtgc cgtgccaccc
     1441 catctctgaa gttagaagta taaaggaaga tctgatacat tccgttatca tctatcgcag
     1501 ccggctgttc gtggccctgt cccgtcaata ttgtcaggtt gtaccttacc tgaaaaccgt
     1561 atatctctat caactcgtcg ttggcggccc ttatctcgtt tcttattttc gtttcgacct
     1621 gcgcgaagag agagatagag acgagaagcc agagtatcag gagcgatcgt actctcattc
     1681 gattgacgcc agaaccttca ggagatcctc tggaagttct gaatctacag cgatcttcaa
     1741 tccagaacta cacgtgaatc cgaggtgctg gcagtgcaga aaataccttt tcaagccgct
     1801 tgcctttttg aagttcctgt tgaaggtcct atcaccgtaa acatcgtctc cggcgacggg
     1861 gcagttgatt tgagccatct gacgcctgat ctggtgcttc tttcctgtgt gaaggcgaac
     1921 cttgagtaac gacacttctc tatagcgctt ctcgagtgag tactcaagca cttcgaggat
     1981 cccttcgttc agggattcta gtctcgcgct ctcgatttgt ggacagcctt taaccagcgt
     2041 gaggtatatc ttctcgactt cgtgttctct gaacaatctt gaaatctccc tggccgaggc
     2101 aggggacttc gcaaacagga taacgccgga ggtgtgcttg tctaacctgt ggacgggatg
     2161 aggttcgaac cccttttctc ttccgtaggc cagcaaacct tcaatcaggg ttataatctg
     2221 tattcccttt cccgggtgca ccgagatacc ggccggcttg tctatcgcta tagtggtatc
     2281 gtcttcgtag agaatatcga gcggaatctt ttggggaacc agttctctgg tctcttccag
     2341 ccttttcatc tttgatgagt cacccaggta ataaacctgc aggacatctc ctaaggaaag
     2401 cctgtgggat tcatccttca cccgtttccc gtttacttta acgttccctt ttctgagaag
     2461 tttgtatatt gaagagagct tcaggtcatt cagaacattt ctcaagaacc tgtccagtct
     2521 ctcgtagtaa ttaccgttgt ccaccttgaa ttctctatag attcaaatca cctctggagc
     2581 aacggggagc ggtggctccc cgtgataatt catccttcaa ctttgagacc cttacttatc
     2641 tgctcaacta tatctccaac ggtggagatg ttctcgatct gttcatcttc gactttaaaa
     2701 ttgaactcat cttcaaaagc cataaccaga tcgaccagat ccagggaatc ggctccaagg
     2761 tcctcggtca aatcggaatc aagcgctatt tcatcctctt caacaccgag tttttcggct
     2821 attatggtct ttaccctgtc aaaaacctcg tcgtgtgtca aaagatttcc tcctttctcg
     2881 ttgaatctgg aaatcgaaac tggagcggaa acctcggtaa aaaaacgtta tcacccgtag
     2941 atttcatgat gctctcatta ttatataata actctgtacg ataaggtttg attccggagg
     3001 ttgtcgatgt ttaattattt cgaagcagcg agttccatga tcaaggaggt tggagtgaag
     3061 ccggaggttg ctctcattct cgggtcggga ctcggctacc ttactggcga gtttcagggt
     3121 ggcgtaagtg tcgattactc gaagatacag ggctttccag tttcaacagt gaaggggcac
     3181 tcgggaaggt ttgtttttgg tagctttcac gatattccct cggttgctat ggaaggacgg
     3241 tttcacttct acgaaggtca cgaaatcaag gatgtcgtat cgcccattta tcttttcaaa
     3301 gagatgggca tcgagcgcct tctgataacc aacgcttccg gaggcatcaa cagaacctat
     3361 tcgcccggag acatcgtagc gataaaagac ataataaatc tcggatttag aaacccgctg
     3421 ataggaccga acgatgacag gttcggtgtc aggtttccgg acatgtcgtc cgtgatggac
     3481 tttaaatggc tttcagggct cagggaaaga ctttcacggg atggacgaga attgaaagag
     3541 ggcacttata tctgggcact cggaccgtcc tacgagagtc cctcggagat cagatcgttc
     3601 gagtttcttg gagccgatat ggtcggtatg tcaacggtgc cggaggtgat agccgccaac
     3661 cactgccgga tgaagattct ggctctctca tgtataacga atatggcttc gggaattcta
     3721 aaagagaaat tgtcgcacga cgatgttgta ggaacggcca acaggataag gcacaggttc
     3781 agtgacgttg ttggcaaggc gctggaagtt ttgaaggaga tcggtcatga atagaactat
     3841 gaacgcggtt ttctcgaagt tgctgcaggc tcaaaatata ctggtctgcg gacatataat
     3901 gccggacgga gattgcgtat cttcggttgt atcactctca atgggactag aaaaattggg
     3961 aaagaagacg accatggcaa tcgactggaa gattccatca atcttttcac cttttccccg
     4021 tgtggaaagg atcatcgatt attcccgtta ttcgactcag ttagaaaatt ccgatttact
     4081 ggttatcgtt gatgcatctt cgccagacag gatcggaagg ttcgaaaggt tacttcgtta
     4141 tggaatgcct tcgattctta ttgatcacca cgcgaccaac acccgcttcg ccaatctctg
     4201 ctgggtcgag ccttcctatt catctaccgc acagatgatc ttcgatctct tgaagttgat
     4261 ggaagtctct tacgacggtg acctggcact gatgaactat cttggaatag caaccgatac
     4321 gggttttttc cgatattcca ataccaacag tacagtgttc gaagccgcag ccgaactcgt
     4381 aaaactcggt gcagatccat gtctggtagc caccgccatt ctcgagactc gaaggctgga
     4441 agagtttttt ctcgagagag acgctataga caatctcagg ctgctttcga acgacaggtt
     4501 cgcttactct tatctcaggg tcgaagattt cgagaggtac tctctggggg aggatgattt
     4561 cggtggtttc gtgggtgatt tgagatccat agcgagcgtg gaagtcgccc tttttgcttc
     4621 ggaatccgtc ggaggcgagg cgcacgtgtc gttacgttcg aagagctatt tcgacgtcag
     4681 ttctgttgcc gtggcatttg gaggaggtgg acaccccagg gccgctggct tcacgctcaa
     4741 gtacgatggc gatctcgaag aggcccttca ggaggccgtc gagttgataa gcagaaggct
     4801 ggagtcacac tcgaaagatg ctacactatg agccaggaaa gaatctgaat gaactgtgta
     4861 acgggccaca tgacgagctg gatcacgccg attagagaaa gcaccaggag gacaagtatg
     4921 ccgtacagct cgtacttcac gagccagttc acatagtttt ccggaacgat caccgcgatg
     4981 atgttggcac cgtccagagg cggtatgggc aaaaggttga agaaggcagt gcttagatcg
     5041 atgaacatta tccagtagat cacgtcggcg aaataactca acgggtagct gctaaggtag
     5101 tcgggcatca gagtggaata cctgtgaagg ccggaaagat agaagatcag gccggccagg
     5161 agaccgacga cgaagttacc aagagaaccg gaaagggcgg tcaatatcgc cttcgagtag
     5221 ccgagctttc taagtttcca gtagttaacg ggatagggtc tagaccaacc gaacttgaag
     5281 agatagtaca tcagcagtcc gacgggatca atccttctta tgaagcccgg tctgctccac
     5341 tcggttctcg ttctgtcgaa atagcgtgcc agatagtaat gcgaatactc gtgagtgaga
     5401 atggcaacga ttatcgccgg tgtcagagag ataaagttcc taagcatctc tatcatcttc
     5461 accgctcctt ccggaaacta caaccgtcaa ctctccaaca ggttcgctcg aggcgaaatg
     5521 ggctattatc tcggagattt ttccgttgag ggattgctga aacttcttgg tcatctctct
     5581 ggcaacgaag atatctctgt cgccaagtat ctccagcaga tctctcaacg tctccaaaag
     5641 tctgtacggc gattcgaaga aaacgatcaa gctctcttcg aacgtccctg cggagatttt
     5701 tctgaaaagc cttcgccggt tcttccctct gggaagaaag cccacgaagg tgaaattcgt
     5761 acccgggaag ccgctcaggg cgacggccgt cgtgacagcg ctcggaccgg gtaccacgtc
     5821 cacttcgata cagttctcgc ggcagattct gaccagcgtt gcaccggggt cggatataac
     5881 aggcattccg gcatcgctca ccagggctat tttgtcaccg tttttaaggc gggcgagaag
     5941 tgccggtatg cgtcgggact ggttatgcat gttgaaggag attatctctc tacgcttcaa
     6001 ttcaagggca ctcatgagat tcgatgccct tctggtgtcc tcggcaataa ccagctccac
     6061 ctctccaagc actctcaggg cacggagagt tatgtcctcc aggttcccta ttggagtacc
     6121 taccaggtaa agtcttccat attccgaatc catcacttct ctcccagggg gaatttcgat
     6181 gccataccgg gcctcctcgg gaagcctttc ggagtttcgg agaccttctt gaaggttacg
     6241 agtttcctgg aggagccgtc cgaaagtctg taattcctct cgtcctgaag ctgcaatccc
     6301 aatttggccg aggccacgaa agcatagcac tcttcatctt tccaacccgg tcctttgtac
     6361 aggtgtatca gacctccgtt ttcaacaagc ggggctgcca gttccagcgc cgcatcgctc
     6421 ctcgccacag ccctcatgac ggcgccgtcg aacctatcga tccctttcgg atactcctcg
     6481 atcctcgagg tcacggccgt tacgttagac agggaaagcg tcgatgaaaa cctctcaatt
     6541 tccctcactt ttttcgcgat tgaatcgaca agtacccacc tggtttctgg aaaggctatc
     6601 gcgagaacca gtccgggaat cccccccccg ctgccgatat cgatatagct cccttctagc
     6661 ttgtagcctt cgaaggccaa cagggaatct tcgatgtgtg ccaccatagc cctctcccgg
     6721 tccctcacgg aggtcaaatt gtggggagaa gagatcaaca aatctaccag ccgttgaaac
     6781 ctttcgcaag tgtcactaga aaaagattcc ccaagattca tctggagccc ttctcaatac
     6841 aggcataagc ctcgtggtta tgaatcgatt cctgactgag aacctcgact ctataccagt
     6901 caacacgcga atccttgttt agtttgagaa cgacttctct ggaaagatcc tccacaaacc
     6961 tcggattatc gtaagagtgt tcagttatga acttctcatc ttcccttttg aggagcgaat
     7021 atgtcggtgc gctggccgag gtttcgacaa tatcgattat ctcttctatc catatcaaag
     7081 agttcatcct tacagaaacc gtcacttctg ccctctggtt gtgggcgccc ctctcgctta
     7141 tctctttcga acagggacag accgtcatta ctggtacgcg aacgcgtaga acgaaatcga
     7201 agtcgccgtc cttcctagat atgaaggagc agacgaaaga gctgaagctt tcgctgttgc
     7261 taaccggggc tttttttctc atgaaatacg ggaactcgac ttctatatgg gcaacatcgg
     7321 cgttcaaagc ctgccgcata tcctcgagaa tagactccat gttcttcgga gtaattttct
     7381 tgtggtgcct ctcgagaacc tctacgaacc tggacatgtg ggtcccccgg aaatcctttg
     7441 gaaggtccac gaacaggtcg aaggttccta ctgtatgctg attgccaaac gccctgtcca
     7501 taactattat cgggtatttg attgatttga taccgaccat gtttattctt atgtttcgtc
     7561 tatctctctc attctgaaca tctctcacga tttatcgccc cttgaatgta tgaagacaaa
     7621 gagttataat cgcaccgtgg aggcgtatcc taattggcta aggagccggt cttgaaaatc
     7681 ggtggggttg acgccccttg tgggttcgag tcccaccgcc tccgccagta accgggagta
     7741 tcccggttat ttttttagcg gaccgaaatc ctccagcggc catatggtca gcataggact
     7801 gcctacaatt gccctttccg gcacaaagcc gaaatacctg ctatcgaaac tctccgtcga
     7861 gttgtcgccc atcatgaacc ggaatccttc gggcacggtc accagaatag aaccggtttc
     7921 gggatcggtc tttatgtacg tctccatacc gagcggagcg agtacggagt cgtagtattc
     7981 ggtgtattct atcagtcctt tatacgcctg aaacaatctg taatagagag agaacctcac
     8041 cgaagcatcg tcgggatggg ccaggccgaa ataaaatctg gtgtcgttga atataccctc
     8101 tttcacgtat ctcctgtttt ccagttttgg cggaacctct ccgttcacgt atagctggta
     8161 aaccggccgg gccgccgtat aatccggggc tctacgcagt tcgaggatgt cgccaggctt
     8221 tccgacgagc cttttgacgt atttcacatg gcccttgaac tccgccgggg agaagaaatc
     8281 catgaacttg tcgaatcctc tcagatattt ctgcgattcg atatccacgt aaggcgtcca
     8341 gaagaccact atatcgccgt aatctggttc tctgtatgag taagttatct tctccacaaa
     8401 aagcctggcg ggcgggttaa tcgttgggat catcgacccc gtcggcacga gcatggtttc
     8461 aaagacgaac aaacgaatta ttgtaccgaa gaccacggca taaaggatcg cctttcccca
     8521 ctctttgatt tcgtgaagaa agcgcgatcc ctttttacca gaagtcttct ccgatttcac
     8581 ggaagccaca ggacttcagt cccttttctc ttttatcttt accttaccct tcacatttct
     8641 tatgtagtat atcttcgctc ttctgacttt accctttctg aggacttcga ccttttctag
     8701 agaaggactg gtgaacggga agattctttc gactcccaca cccgccgctc caactcttct
     8761 gacagtgaaa gattttcccg ttccgctgcc ccttattccc atgacaattc cttcgaaagc
     8821 ctgtaccctc tctttatttc cttcctttat tcttacacta acccttacgg tgtcacccgg
     8881 gccgatttcg ggaaggtctt gcctcataaa ttcattctcc agagatctta tgtactgatc
     8941 catgtttaat ccctcctttt ttctgtatct tcacctataa gtctatccag tataacagcc
     9001 accgccgcac gaaccgaaag atggttgaaa tcggaattag cgcggatcgg ttcaagagcg
     9061 aacttgcata gtttcaaaat ctcttcgggt aatccccagc tggttccgaa gagaaggaga
     9121 tggggaatct ccgaagtcct cagcagccct gacatttcgc cataggaaag tctgtcgggg
     9181 cctctcttgg ccgaagtgaa aaccaggtgt ggtctgacgc cttcgacact ctctatatct
     9241 tcaataacat cttcgagata ggaggccttc ctcacaacgg ttaaagcttc actcctgctt
     9301 ggattgtact ctctgccaaa ctcttccagc cagtaactaa gaacattgtc cactatctcc
     9361 cgctgcgcgg gaaggttgct caccacatag taccctttga tgttgtaagt cctgcacgtt
     9421 ctcgatatat cgtgaatatc gagattggtt acggcgctgg agactatatt cgcgtgtcta
     9481 ccgagaaccg ggtagtgcat caaagccagg tatatgttat tcaacattct ttctcaactc
     9541 ctcaaaaagc gagaggattg cctttttgtc gtacaggtcc agttcgtgtt tcaaaaagat
     9601 atcgggtcgt ctttccatgg ttctctcaag actttctttc cttctgaata tctctatctt
     9661 ttcgtgatcg ccgctcaaga gaacatccgg tacctcgagc cccctgacgt ttcttggccg
     9721 ggtgtagtga gagtgatcca gaagatccct gtaaaaggaa tcgttaacaa ccgattccat
     9781 atctcccacg acacctggaa caaacctgga gagggcctcg accatcaaca gcaccgggat
     9841 ctctccgccg ctcagaacga aatcgcctat cgagagttct tcgtcaacga tggacatagc
     9901 cctttcatct atcccttcgt acctgccgca gatgaacaac agcctctctt tcttgcttag
     9961 gtctctagcc ctggcgttgt cgaagatctc gccctgcggt gatgtgtata tcacataagg
    10021 ggctggaccg tcggtcctca gagtgtcaac cgcccttagg ataggatcgg ccttcatcac
    10081 caggccgctc ccgccgccga agggataatc gtccgtcgtt ctgtggcgat cgtccgtgta
    10141 atcgcggaga tcgaccgcgc cgaattcgat caaaccgctc tcgatggctc tggagattac
    10201 tccccacttc agaaccgctt cgaaaagatc ggggaatatc gtcagcacat cgatcttcat
    10261 ctaaaccgcg tcaggagaca cagggcttca gcccggtgag gaatgacgct tccctccttt
    10321 cacataatag tgatgatga
//
LOCUS       DAOOLY010000066        10052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7677_length_10052_cov_5.036511, whole genome shotgun sequence.
ACCESSION   DAOOLY010000066 DAOOLY010000000
VERSION     DAOOLY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_10052_cov_5.036511"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /locus_tag="PLD15_04845"
     CDS             <1..1814
                     /locus_tag="PLD15_04845"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPB63263.1"
                     /translation="KTYSIYFTVKKIMISITAVPPDGGVFEGDGAYDYGDLVTIVATP
                     SIHHTFNGWYLDEPDELTFNESNDPVYTDPIVSFHASQDVDLLVEFDPMQYTFTVVAD
                     PHEGGEVTGGGTLNYGDSLNLSALPNTGYGFEGWWIDSYMFSDQAVDSVSTVEILNHT
                     ECITCTEHIVKGKFNPSAPQWQLDVTIEATPIYGGETWGEGLYPYGVDITVGATPNRH
                     FDFDGWYYGGEQLSEGATYTFPANEWYTEGVTYTALPLQGVFVDVLYKFNAVAEPPEG
                     GQVSGGGICTYGDYLAFLASANSSYSFLGWYYGEEYMSGSPSFTLNTGSFLDTHTLPI
                     TSTEHSFTFTGKFEPQTPPPGWLMITIDASPTGKGELSGAGKYPYGEDVTLGATPINY
                     YDFGGWYTEGNLLSEELPYTFNTSDWLPTQGVTDTLPIVAWFPPIEYQFNVVADPPEG
                     GEVMESGKFRHGDPMVLSAIPNDNYLFKNWTFEGAEFSDQPVWEGDSTFFLARSTCIG
                     CNEFTIVGNFDLDVPDSITVDLRPEPPEGGQVSGGGQFPKGSSTVISALPNPGYGFGG
                     WYYDPCGSFFSDYQSLTIGNLQEDWSLFASFYPLSGN"
     gene            complement(2023..4521)
                     /locus_tag="PLD15_04850"
     CDS             complement(2023..4521)
                     /locus_tag="PLD15_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB63264.1"
                     /translation="MKRYTELSGKYLSRRLRRTFYTLLGVALAVAFITAIMITFESIE
                     TSEIRALEESVGAYHGKVEKATDSEILDLSAHVLTKGLGIQKIAGSIDFPEEKASLLI
                     EEADGLTLHLRGKRVTEGRLPERPGEIAVDSFAAKLLGISSKIGKRVSLNINGNERVF
                     EVVGIISTARQESSLSRAFVSVSAQEFSAIASKSEVAKNVYFTVDAENKMLRIAAQKV
                     ASDLELTDRARYNGSLIYHLETLSPVNWPAVVLGLLVAVASMVSIYNTVHISVLERIR
                     EFGLLRAAGATPAQIRKVVLRESILVSVLGIPIGLAAGVLLSFAVALYAGSRLTGLNG
                     FATSITPLSLVLGAVMGFLSVVVSSLIPAFRAGKISPVEAMRQYGVESSGREIRKVAA
                     GESATGEKIPLKLAKRNMRRNLRTTVISVVSMTIAATLFIVFSYFAGNFDTERLARGV
                     VKSDFSIRVTGMYDDGPYGKTIEELQSIEGVETVAAAKFLTGRLLSEYEDPEMESKTY
                     VTVPNPQAGMRVTTLDQSGMYMALLAYNEAGIELMKSKVISGSIDLARMTSEPLVIID
                     VENSNRHNIKAGDRVLLRTYYLTESMVQLPINSEFTVGAVVQELPSVAYTVAGGILVA
                     CSEEIIDGLRPAGNDVHLTMMDYVDHYSYVDIYLRRGANPENIEPVVEETAKRYRNST
                     FISYSQYRKETENAIRTLSTLVYGLIAIVGFIGICGITNTINTTIILRRREFGILRAV
                     GMTGKQLKAMLVFEGLILGIISAISSVVLGVILSYAVYSLLKTEIGHLSWSIPWLGIV
                     LAVAGTLAVGVLTTLASSRKVTSLSITESIRTIE"
     gene            complement(4521..5243)
                     /locus_tag="PLD15_04855"
     CDS             complement(4521..5243)
                     /locus_tag="PLD15_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63265.1"
                     /translation="MEVLRATGLRKVYGSNASAVTALKGADLSIEEGEFVAIVGPSGS
                     GKSTLLHLLAGLDRPTAGSVYIDGKNLYGMSDTQLSIFRRRRIGFVFQFFNLVPVLTA
                     RENIELPLILDERKVDQPYLNELLRLMKLEDRTDHLPSALSGGQQQRVAIARALITKP
                     VIVFADEPTGNLDSKTSQEVMDLLKISARKFHQTLVIVTHEEDIAERADRIIALEDGE
                     IVSDYSNSDVGSVFSSALGGQK"
     gene            complement(5340..6596)
                     /locus_tag="PLD15_04860"
     CDS             complement(5340..6596)
                     /locus_tag="PLD15_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB63266.1"
                     /translation="MFRSLFRERQNRVIAMIGIALSLSIALIAIVTLYLSLEDHGRHL
                     YEREVSIIGRLVSLGIITHDDVAKMLSEERSEDYEAALELLKPFGYTEEPPGSFEFSD
                     GAFRSKVIVIASLSSITLAILWFSLLTLVAKKQSDFLKDTSLRLDALMNGRYNSESVY
                     YGEGDTAILSAQINALTRRLEKTMESVNFERDKMRGFISFISHELKTPLASLKTMNEL
                     LIDGKEMEREQMEEFLKRSGEDIERMEWLIGDVLNIARIESGAIRFNFKKADLAELAK
                     DVARRYSEIAKSKSLKISVGAESTATVFCDERWLSQALDNLVKNAVNYSPDGGTVSIN
                     IFRSEAYAYLTVTDEGPGIAAGDTPKIFQSFYRGSSAVGSRKGTGLGLALAKAIIERH
                     DGDIKLKSSTEKGSIFAIELPIRQEE"
     gene            complement(6597..7289)
                     /locus_tag="PLD15_04865"
     CDS             complement(6597..7289)
                     /locus_tag="PLD15_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB63267.1"
                     /translation="MLSILLVEDDKNLANGIRYALEQEGWSATVAGNVSKALELMENG
                     HFDLALLDVMLPDGNGFELCKKIRGRSDMPVIFLTARDEEVSVVMGLESGGDDYVTKP
                     FRLRELISRIKANARRIQKIHPVEGSALLESGPVKLDTAQIRAYRSGKEIFLTPTEFR
                     ILRSLIENSGAVLTRDQLLEKVWNIDGEFIEDNTLSVHIRKLREKVEEDPSKPKLIET
                     LRGLGYRWNGEK"
     gene            complement(7654..8517)
                     /locus_tag="PLD15_04870"
     CDS             complement(7654..8517)
                     /locus_tag="PLD15_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPB63268.1"
                     /translation="MKKAVVVVVSIISIAVLVIVLVIALKPKKDSPPTESIVDMILVE
                     KGTFTMGDTWGSGWDEERPAHEVTISYDFHIGKYEITFALYDLFCQETKRALPGDEGW
                     GRGQRPVINVSWMDAVAFCNWLSEREKLPKAYDANGYFLDKDGKVTTDPSKVVGYRLP
                     TEAEWEYGARGGNKSEGYIYSGGNEPDLVAWYSDNSGDMTQEVGQKSPNELGIYDMSG
                     NVSEWCSDAYDAFFYRRSPAKDPYNQALGNKSIRGRTWWDGVVRVTSRSYNSPNSAAN
                     ICGFRVCRTFN"
     gene            8968..9552
                     /locus_tag="PLD15_04875"
     CDS             8968..9552
                     /locus_tag="PLD15_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB63269.1"
                     /translation="MTIEEINTRERIFRATLEFIREGKDAHKLTTRQIGDRAGVNPSL
                     INYHFQSKENLIGQVVGTMMGEIIGQLRRSDAIESDPETRLREILLTTADAAFEFYNV
                     SRIAIAGELKEGCRNSCQMVLPLLQEIFKGYTFSDLNVIALQLMLPFHHIVVDPVLYG
                     KYLGADFFDEKQRRGKINQMVDLVLARYDKEAKK"
     gene            9549..>10052
                     /locus_tag="PLD15_04880"
     CDS             9549..>10052
                     /locus_tag="PLD15_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63270.1"
                     /translation="MKQAGISKAGSKIVNRTILVVVATMAIIFGIGGIGHGFFEALQG
                     FTPTNGLVIHAIGEANRMWEYGNEPAFTVIPNFLITGIASMAVGLAVIVWSVGFLHRR
                     NGPTVLLLLFVLLFMVGGGIGQVVFFSIIWVFSTFIHKRLKPSKSEKTGGLRKKTARF
                     WRPFLVAS"
BASE COUNT         2332 a         2585 c         2534 g         2601 t
ORIGIN      
        1 caaagactta ttcaatctac tttacggtga agaagataat gatctcgatc acggccgtcc
       61 cacccgacgg cggagtattc gagggagatg gagcatatga ctacggcgat ctcgtaacaa
      121 tagttgccac gccatctatt catcacactt tcaacggttg gtatcttgac gagccggacg
      181 agttgacctt caacgaaagc aatgaccctg tttatacaga tccaattgtc tctttccatg
      241 cttcgcagga tgtggatcta cttgtagagt tcgatccgat gcaatatacg tttacggttg
      301 tggcagatcc tcatgagggt ggagaggtta ccggtggagg aactctcaat tacggtgatt
      361 ctctaaatct gagcgcatta cctaatacag gttatggctt tgagggatgg tggatagaca
      421 gctatatgtt tagtgatcaa gctgtagact cggttagcac tgtcgaaatc ctcaatcata
      481 ctgagtgtat aacgtgtacc gagcacatcg ttaaaggaaa gttcaatcct tctgcgcccc
      541 agtggcaact ggatgtgacg atcgaggcaa caccgatcta cggaggagaa acctggggtg
      601 agggactgta tccgtacggc gtagatatca ctgttggagc tacgccgaat cggcacttcg
      661 atttcgatgg gtggtattac ggtggagaac agctaagtga aggcgctacc tacactttcc
      721 cggcaaatga atggtacacc gagggggtaa catacaccgc gcttccctta cagggagtgt
      781 ttgtagatgt actatacaaa ttcaacgccg ttgccgaacc tcccgagggg ggacaggtga
      841 gtggtggcgg tatctgtact tacggtgatt atctggcatt cttggcttct gcgaattctt
      901 cttattcctt cctgggatgg tactacggag aagaatatat gagcgggtcc ccgagtttca
      961 cccttaacac cggcagcttc ctggataccc acacgcttcc cataacaagc acagagcaca
     1021 gcttcacttt taccgggaag tttgaacccc agactccacc accgggatgg ctgatgatta
     1081 caatcgatgc ttcgccgact ggcaaaggcg agttaagcgg cgccggaaaa tatccgtatg
     1141 gagaagatgt cacgctggga gcaacaccga taaattacta cgatttcgga ggctggtaca
     1201 ccgaggggaa tttactgagc gaagagcttc catacacatt caatacgtcg gattggctgc
     1261 cgacacaggg tgtgaccgac acccttccaa tagtcgcctg gttcccgccg atagagtatc
     1321 agttcaacgt ggtggccgat cctcccgaag gcggggaggt tatggaaagc gggaagttca
     1381 ggcatggcga ccccatggtg ttgagtgcga ttcccaacga taattatcta ttcaaaaact
     1441 ggacattcga gggagccgag ttcagcgatc agcccgtatg ggaaggagac tcgacattct
     1501 tccttgctag atcgacctgt ataggctgca atgaattcac catagtcggt aatttcgatc
     1561 tggatgtgcc cgacagtatt accgtcgact tgagacccga gccgccggaa ggcggtcaag
     1621 taagcggtgg aggtcaattc ccaaaggggt caagcacggt tataagcgcc ctgccgaatc
     1681 ccggatatgg gtttgggggc tggtactacg atccatgcgg aagttttttc agtgactacc
     1741 agtctttgac aatcggaaac ttgcaggaag actggagtct tttcgcttcc ttctatccgt
     1801 taagtggcaa ctgaaatgcc gtatttcaag aagctaagac ttgcttctcg atcttcagca
     1861 gtcggtcaat agaaggcaaa atccgattca tccaggttac taaaaagcct gaggattcac
     1921 tccccaggct ttttctctcc gcaaaggctg ttctgaagac gtcgcaattc ttatatcaga
     1981 tttgcatatt ccatagggaa ttgttttgat gagaatcgaa tttcactcta tcgttcttat
     2041 cgattctgtt atgctgagag aggtcacctt tctggacgag gcaagcgttg tcaacactcc
     2101 gactgcaagt gttccggcaa ctgctagaac aatccctagc cagggtatcg accagctcag
     2161 atggccgatc tctgttttca ggagcgagta cactgcgtaa gagagaatca ctccgagaac
     2221 gaccgacgag atggcgctta tgattcctaa gatcaaccct tcgaatacca gcatcgcctt
     2281 gagctgtttt ccggtcatgc cgaccgccct gagtattccg aactctctac ggcgcaatat
     2341 gatcgttgtg tttatggtgt tggttatgcc gcagatgcct atgaagccga ctattgcgat
     2401 caatccatag accagcgtcg aaagagtcct gatagcgttt tccgtctctt tcctgtactg
     2461 gctgtaggag atgaaggtcg agtttctgta ccttttagcg gtctcctcca ccaccggttc
     2521 gatgttttcg ggattagctc ccctgcgtag atatatgtcc acataagagt aatgatccac
     2581 gtagtccatc atcgtgaggt ggacgtcgtt gcccgcaggc ctcagcccgt cgattatctc
     2641 ttcgctgcat gctaccaaaa taccacccgc aacggtgtag gccacagaag gaagctcctg
     2701 aaccaccgcg ccgacggtga actcgctgtt tataggcagc tggaccatcg attccgtaag
     2761 gtaataagtc ctgagcaaca ctctgtctcc tgcctttata ttgtgcctgt tgctgttctc
     2821 gacatctatg atcaccagag gttcggaagt catccgggcg agatctatcg atccggagat
     2881 cactttcgat ttcattagtt cgattcccgc ctcgttgtag gcgagaaggg ccatatacat
     2941 ccccgactga tcgagcgtag tgactcgcat cccggcctga gggttgggaa cggtcacgta
     3001 ggttttgctt tccatctccg ggtcctcgta ttcgctgagc agccgtccgg tgaggaactt
     3061 cgctgcggct accgtctcga ccccttctat tgactgcagc tcttcgatcg tttttccgta
     3121 gggaccatca tcgtacatgc ctgtgacacg tatcgagaag tcggacttca cgacgccccg
     3181 ggccagtctc tcggtatcga agttccccgc aaagtacgag aaaactatga agagagtcgc
     3241 agcgatcgtc atcgagacga cggagatgac agtggtgcgg aggtttcttc tcatgttcct
     3301 cttggccagt ttcaggggga tcttttctcc tgtcgcagat tcgccggccg cgaccttcct
     3361 gatttcccgt cccgatgact cgactccgta ctgtctcatg gcctcgacgg gagatatttt
     3421 gccggcgcga aacgcgggaa tcagcgatga aacaaccaca gacagaaatc ccataacagc
     3481 tccgagcaca agcgagagcg gtgtgatcga ggtcgcgaag ccattcaggc cggtcagccg
     3541 ggagccggca tacagagcca cggcgaatga aagcaggacg cctgccgcca ggccaatcgg
     3601 gatgccgaga acgcttacga gtatcgactc tcgaaggacg acttttcgta tctgggcagg
     3661 agtcgccccg gcagccctta gaagcccaaa ttcgcggatc ctctccagca ccgatatgtg
     3721 aaccgtgttg taaatggaga ccatcgaagc gacggctacg agaagcccca gaactaccgc
     3781 gggccagttc acaggagaga gagtctcgag gtgatagatc agagagccgt tgtacctcgc
     3841 cctgtccgtt agttcgaggt cggaagccac tttctgggcc gcgattctta gcattttgtt
     3901 ttccgcgtcg acggtgaaat agacgttctt cgccacttca gatttcgaag ctatcgcgga
     3961 gaattcctga gccgagacgc tcacgaaagc cctcgacagg gagctctcct gtcttgccgt
     4021 cgaaatgatc ccgacaactt cgaaaacccg ttcattcccg tttatattca gtgatactct
     4081 cttaccgatt ttcgaagaga tacccaaaag cttggcggcg aaggagtcaa cggcaatctc
     4141 gcccggcctt tcggggagtc ggccttcggt tactctcttt cccctcaggt gcagggtcag
     4201 cccgtcggcc tcttcgatca gcagtgaggc cttttcttcg ggaaagtcta tggaaccggc
     4261 tatcttctgg attccaaggc ctttggtcag tacgtgggcg ctcaaatcca gtatctcgga
     4321 atcggttgcc ttctcaactt tcccgtggta ggctccgaca gattcttcta gcgcccgtat
     4381 ttccgaggtt tctatacttt cgaaggttat catgatagcc gttatgaaag ctactgccag
     4441 cgccactcca agaagggtgt agaaagttct tctgagtctt ctggaaaggt actttccgct
     4501 caattcggtg tagcgtttca tcacttctgc cctccaagag cggaggagaa tacgcttccc
     4561 acatcgctgt tggagtaatc ggacactatc tctccgtctt ccagagcgat tattctgtcg
     4621 gccctctcgg cgatgtcctc ctcatgggtc actatcacca gcgtctggtg gaacttcctc
     4681 gcactgatct taagcaagtc catgacctcc tgactcgtct tgctgtcgag attgccggtc
     4741 ggctcgtctg cgaagacgat cacgggtttg gttataaggg cgcgggctat cgccaccctc
     4801 tgctgctgac cgccggacag ggcagaaggc aggtggtctg tcctgtcctc gagtttcatc
     4861 agcctgagaa gttcgttgag atatggttga tcgaccttcc tttcgtcgag tattagagga
     4921 agctcgatat tctctcttgc agtcaacacg ggaaccaggt tgaagaactg gaagacaaag
     4981 ccgatccgtc tccttctgaa gatagataac tgcgtgtccg acatcccgta caggttcttc
     5041 ccgtctatat acacgctacc ggccgtggga cggtctagcc cggcgagaag atgaagaagc
     5101 gtgctctttc ccgagccact gggacctacg atggcgacga actcgccctc ttctatggag
     5161 aggtcggccc ctttcaatgc ggttacggcg ctggcgttcg atccatacac ttttctcaat
     5221 cccgttgctc tgagtacttc catggtatgc ctccttaatt gtagcttcca ctgagaatat
     5281 aaggagtctg tcttatgacc ggatgaagaa gaagcttaag aaatcgcaag atggagaact
     5341 cactcttcct gtcttatcgg cagttctatt gcaaagatag atcccttttc agttgatgat
     5401 ttgagcttta tatcgccgtc gtgtctttcg atgattgctt tcgcaagagc gaggccaagg
     5461 ccggtaccct ttctcgatcc gacagccgag ctaccccggt agaagctctg aaagatttta
     5521 ggtgtatccc ctgcggcaat cccggggcct tcgtccgtga ctgtaagata ggcgtaagcc
     5581 tcgctcctga atatgtttat cgacaccgtg cctccgtcgg gggagtagtt gaccgcgttc
     5641 ttgacaagat tgtccagggc ctgtgaaagc cacctctcat cgcagaagac cgtggccgtc
     5701 gattcggccc cgacggagat cttcaggctt ttgcttttgg ctatctccga gtatcttctg
     5761 gctacatcct ttgccagttc tgccagatcg gccttcttga agttgaacct tatcgctccc
     5821 gattcaatcc tcgcgatatt cagaacgtcg ccgatcagcc attccattct ctcgatatcc
     5881 tctccgcttc ttttgaggaa ctcttccatc tgttctcttt ccatttcctt tccatctata
     5941 agcagctcgt tcatcgtttt caaagaggcg agcggagtct tgagttcgtg ggatatgaag
     6001 gagatgaagc ctcgcatttt gtcacgttca aaattgacgc tttccatagt cttctcgagc
     6061 ctcctggtta gcgcgttgat ctgcgccgag aggattgctg tgtcgccttc tccgtagtag
     6121 actgattcac tgttgtacct gccattcatc agggcgtcca gtctcagaga tgtatctttg
     6181 agaaagtcgg actgcttctt tgcgacaaga gtcaatagcg aaaaccagag gattgccagt
     6241 gttatagagg agagtgaagc tatgacgata accttcgatc gaaaggctcc gtcggagaac
     6301 tcgaaactgc cgggtggctc ttctgtatag ccaaaaggtt ttaacaactc cagcgccgct
     6361 tcgtagtctt ccgatcgttc ttcagagagc atcttcgcta catcatcgtg cgtgataatg
     6421 cctagcgaaa ccagcctccc gataatcgat acttcccgct cgtaaagatg gcgcccgtga
     6481 tcttccagag aaaggtaaag agtcacaatc gcgatcagcg ctatcgatag cgagagagcg
     6541 atgccaatca tggcaattac tctgttttgt ctttctctga acagggatct gaacatctac
     6601 ttctctccgt tccacctgta accgaggccc ctcagcgttt cgatcagctt cggctttgac
     6661 ggatcttctt ccactttctc cctgagtttc cttatatgaa ccgacagggt gttgtcttcg
     6721 atgaattctc cgtcgatatt ccagaccttc tcgagaagtt ggtctctcgt caggaccgca
     6781 ccgctgtttt caatgaggct tcgaagtatt ctgaactcgg tgggggtgag gaagatctcc
     6841 ttgccggacc tgtaagccct gatctgggcc gtgtcgagct tcacaggacc ggactcaagc
     6901 agagcgcttc cttcgacagg gtgaattttc tggattctcc tggcgttggc cttgattctg
     6961 gagattagtt ctctgagtct gaaaggcttt gtcacatagt cgtctccgcc actttccaga
     7021 cccatgacaa cactcacttc ctcgtccctg gcggtcagga agatcaccgg catatccgat
     7081 cgcccgcgga tcttcttgca cagctcgaac ccatttccgt cggggagcat gacatcgagc
     7141 agcgcaagat cgaaatgtcc gttctccatc aattcgaggg cttttgaaac gttcccggct
     7201 acggttgcgg accacccttc ctgctctagc gcgtagcgta taccgttcgc aaggtttttg
     7261 tcgtcttcga cgagaagtat gctcaacaaa cgcgtcacct ccagatggct atagccggcc
     7321 ttcgtaggcc cggcgcttct attatatatg cctgagagag aaagagaaga gatgtgaaaa
     7381 ccagcaacga gaagaagtgc tgtcggtagt tcgctgcact caagagagga cgagaattga
     7441 taatcgtcgg ccgtcatcga tccacagttc tccgagaaga gcctttattc agaagagaag
     7501 cgcgagatct ctgtattggc gttggctcgt tggccagcgt aacatcactg ccggatgccc
     7561 ttattaatag tcttcgagat tttttagatt tcttggtaca tcaaaagacc gatagaaact
     7621 tcaaaatagg actctcagac ttctttccgg gagttagttg aaagtccggc agaccctgaa
     7681 accgcaaatg ttggctgcac tgttcggtga gttgtagctg cggctggtta ctctaaccac
     7741 gccgtcccac caggtccttc cacggatcga cttgttacca agcgcctgat tgtaaggatc
     7801 ctttgccggg ctccttctgt agaagaaggc gtcatatgca tcggaacacc attctgatac
     7861 gtttccgctc atgtcgtata ttcccaattc gtttggactc ttctgtccga cttcctgtgt
     7921 catgtctccc gaattatcgg aataccacgc tacaaggtcc ggttcgtttc cacccgagta
     7981 aatgtatccc tcgctcttgt ttcctcccct tgcaccgtac tcccattcgg cttcggtagg
     8041 cagcctgtat ccaactactt ttgaaggatc tgtcgtcact tttccgtctt tgtccaggaa
     8101 gtagccattc gcatcgtatg ccttgggaag cttctctctc tcgctcagcc agttacagaa
     8161 agccactgca tccatccagc tgacgttgat caccggcctt tgcccgcgtc cccagccttc
     8221 atctccaggc agagctctct tcgtctcttg acaaaacaga tcataaagag caaaggttat
     8281 ctcgtacttt cctatgtgaa aatcgtagga gatagttacc tcatgggccg gtcgctcttc
     8341 atcccagcca gatccccatg tgtcgcccat tgtgaatgta cccttctcca ccaggatcat
     8401 gtccacgatg ctttcagtgg gcggagagtc ctttttgggt ttcagtgcta tgacaagcac
     8461 aataacgaga acggctatgg agattattga gaccacaaca actactgcct tcttcataat
     8521 atcgcctcct aaagagtggc tccacacctg gcagaggcag taagtctgaa gattagtctg
     8581 gaagaaacgg catgggacct cgtttcaata tatcaccggc ctttgggtga gaaaagaaat
     8641 ccacacggtg caagtcaaac gactttgtaa cactgttggg tttttccggc tgcattgtgc
     8701 tgaactgaga ccatgactta atagaagcag cataagcaga aaaaaatagt catcccaaaa
     8761 agagccagcc gcactaagta aggtaattca ttgcttatca ggggccaatc ttgcagcaaa
     8821 atatgttctg gttttcaaca aaatctgtgg ctccttcagt tgcgataaaa ctagccccct
     8881 tgacaaatct atatggatga atataatata tttgaacacg ttcgaaatgt attttaatta
     8941 tgttcaaata cagaggaggc gaaccgattg acgatcgaag aaatcaacac cagggaaagg
     9001 atcttcagag ccacgcttga atttattagg gaagggaaag acgcgcacaa actcactacg
     9061 aggcaaatag gcgacagggc tggagtgaat ccttcgctga tcaactacca tttccagtcg
     9121 aaggaaaatc tcataggtca ggttgtgggg acgatgatgg gagagatcat cgggcagttg
     9181 cggcgaagcg atgcaatcga aagcgatccg gaaaccaggt tgagagagat actccttacc
     9241 accgccgatg cggctttcga attttacaac gtgagcagga tcgccatagc cggcgagctg
     9301 aaagagggct gcaggaactc ctgtcagatg gttttaccgc ttttgcagga aatcttcaag
     9361 ggttacactt tctcagatct gaacgttatc gccctgcagc taatgcttcc gttccatcat
     9421 atagtcgtcg atcccgttct ttacgggaag tatctcggcg ctgatttttt tgacgagaag
     9481 cagcgaaggg gaaagataaa tcaaatggta gatctggttc tggccagata cgataaggag
     9541 gcaaagaaat gaagcaagcg ggtatttcaa aggcaggttc gaagattgtc aatcgcacga
     9601 tactggttgt cgtagcgact atggcgataa tcttcggtat tggcggcata ggccacgggt
     9661 tcttcgaggc gctgcagggg ttcaccccga ctaacggatt ggtgatccac gcaatcgggg
     9721 aggccaacag gatgtgggaa tacggcaatg agccggcctt cacagttatt cccaatttct
     9781 tgatcacagg gatcgcttcc atggcggtgg gactggccgt gatagtctgg tcggtcggtt
     9841 tccttcacag aaggaacggt ccgactgtgt tgctgctgct ctttgttttg ctgttcatgg
     9901 ttggcggggg aataggacag gtggttttct tctctattat atgggtcttc tcgactttca
     9961 ttcacaagcg gttgaagcct tcaaagagcg aaaagaccgg cggccttcga aaaaagacgg
    10021 cccgtttttg gcggcccttt ttggtggcct ct
//
LOCUS       DAOOLY010000067        10021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7711_length_10021_cov_5.284668, whole genome shotgun sequence.
ACCESSION   DAOOLY010000067 DAOOLY010000000
VERSION     DAOOLY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7711_length_10021_cov_5.284668"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1067
                     /gene="minD"
                     /locus_tag="PLD15_04885"
     CDS             255..1067
                     /gene="minD"
                     /locus_tag="PLD15_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPB63271.1"
                     /translation="MAKVFVVTSGKGGVGKTTVTANIGCALANKGAKVCLIDADIGLK
                     NLDIALGLENRVVHTILDVANGKVTASEALVRHKQIKGLYLLAASQIATKEMLSPEDM
                     KKIVAEIYPKFDYIIVDSPAGIERGFRNAIAAAERALVVTTPELPAITDADRVIGLLE
                     NSGMLESNIRLLINRFKIQMVKRGDMLTREDIQGNLAIDLIGIIPDSDEVIVATNKGV
                     PVVLNGNGEGIGRVFNNIAQRIKGEAIPVENDIQEQGSRGFLEFLKRLFRRN"
     gene            1084..1359
                     /locus_tag="PLD15_04890"
     CDS             1084..1359
                     /locus_tag="PLD15_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPB63272.1"
                     /translation="MFFGLFRKKKKTEGSRKEAKDRLESITGGRRYSVPVREVIPQEL
                     LQSSGKDMIYQIKAYVADKYKVKEENVKVQLEEHNGYVVIITNVVFH"
     gene            1363..1752
                     /locus_tag="PLD15_04895"
     CDS             1363..1752
                     /locus_tag="PLD15_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB63273.1"
                     /translation="MVYTIKSGKRVEFIDITDLVHQSLRESRVSHGVVNVFVPHTTAG
                     ATVNENSDPNVVEDMINWLGRLVPVSEEFRHFEGNSDAHIKATLVGSSVNIPFSDSEL
                     NLGVWQAVYFCEFDGPRKRNVVISVIG"
     gene            1805..4558
                     /locus_tag="PLD15_04900"
     CDS             1805..4558
                     /locus_tag="PLD15_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB63274.1"
                     /translation="MRKRSLLFYVVFIFGSSLFAIKGFTWDMGLPKFYFASVMISILI
                     IFLGMKAVRSPWELSMAFPQIIALMLAAYSCLTTLLLLESLPEVFPVSLGYAMNFLLF
                     VVFSVLISKDSGDKLLIFLQIFIVAGLVIALDALFAFYSGTSLLWGTESAPLSRGNLA
                     SVIGNVNFTTDLMGMLLLPVAFLVVTKKPVWKRETLRRVFYLVVFSVLLVVILIGQTR
                     SVYYSVIGSLIFVVLFSTVLLIRFRPKGVFTAFSRLFLLLLLLVTVAVVWTYSGDNFL
                     TQGSFSFSERLTYTFEDSISVDSRLLQWEAALEQWKGSKVLGTGFGSYKYFSTENMGV
                     VLATKPQYMYAAGLNSIRAHNEYTQMLGETGVVGLSLVFLFIFSMAIHSWKVVSRTDS
                     PKNALEYLFLVAGLLILFIDSILSFPAHLMPNALLGVFLFGFAMRREYYGGRRLTVKF
                     GGAVSLIAVVFSLGTSVLMSRNFISEGLFTRGYIAYKNLENINPQIPGLLESISSIKV
                     EKELLQNFTGTYTALATDTYVTSRENRLRERYPVAPERLLHDMAVQEQELAYKSAQSQ
                     LDSRLRAASSALMNARQDGNENFYRALRNLALSRELSPGQYMADVYFGYLYLTAQRKE
                     DFRLKLSMSQGNLEAVYREIFSREDAFSAWLNDSGQTGGLLNEISLDHAFLRNLPDAI
                     FHTVSSTDLQSLLDGLDSDLLIDFQLSLDAIDALLRSLSTAPDLQVVRNTASLLFRVM
                     IDSSGVSAELKEIVPILKDPATLNGIIADLDDLSSSHRREMVALYEAAIANSPGSWLK
                     DDPNIYEEYSRNLLMVYGEGELAKVLEIAESELFAWSYMKSQNVVPDGVLKVLRSFKE
                     DLKIGWVEALYGEAAAWCEERIAFLKPELENQDIPNVEREKLNVFLRRADGFIKLYKY
                     FLE"
     gene            4656..5387
                     /locus_tag="PLD15_04905"
     CDS             4656..5387
                     /locus_tag="PLD15_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63275.1"
                     /translation="MLEVKDIKLVRDGRAILNSLSAQFEKGKVYAVLGNNGVGKSTLS
                     YVLMGLESHRDYDGSIVLDGDRINGLSVPERAKKGITLGWQEPARFTGLTVKEYLTLG
                     GKMKLSSEELLEILAVVGLNGDYLDRFVDDTLSGGERKRVELASIMIVNPHVVILDEP
                     DSGIDIMSMEMIENVLEKLKDKGTTVIIITHREEIARMADEAYLLCGGRMLASGEPEE
                     IVQFYRKLCDKCQHINEPVRGDVST"
     gene            5384..6340
                     /locus_tag="PLD15_04910"
     CDS             5384..6340
                     /locus_tag="PLD15_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPB63276.1"
                     /translation="MIESSYEREFKAIAQEYERSGGNVSDFLRKDIVSIIVSGNKIIG
                     RNTVEGVHLRAKELDNGVEVWLDIDDGIVVDNPIHLCTGYLKPEGVQTVLIHNRIGDG
                     SKVKFISHCVFPSGKNFTHSMVADTNVGKGAEMLYEDTHMHSKDGGVTVMATYRTRVE
                     EGGRFQNLFYLTKTRVGKLYVKMYVDLEKNASAHLESKVYEREDDYLEIDEEINLNGE
                     GSSGIAKTTVFATDQSKAKIINKAYGNAPFSRGHIECNEIIKGDAVEVGTVPELYVKN
                     EKAELTHEASIGRVNVKQMETLMSKGLSEEEATDLIIKGMLR"
     gene            6362..7390
                     /locus_tag="PLD15_04915"
     CDS             6362..7390
                     /locus_tag="PLD15_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPB63277.1"
                     /translation="MKYLEDYTIRKILELVNIPSPSGFTEKIIEFVATQLREMGFEPR
                     ITRKGALVVELGGSGNPLVLVAHVDTLGAMVKSLKSNGRLEITNVGGFTMNSIENENC
                     TIHTKSGKTYTGTIQSLAPSVHVFENARTLERKMSNMEIRVDEEVSCQSDLEKLGIEA
                     GDFISFHPRAVVTDSGFIKTRHLDDKASAAILLGLARKVSIGELECGRKVYLFFSNYE
                     EVGHGASAAMPEDSEEIISVDMGAVGDTLKTDEYVVSICAKDSGGPYDGQTVKKLIDA
                     ARRAGADYRVDIYPFYGSDVEASLRAGYDVMFGLLGPGVEASHGYERTHCKAVDNTLK
                     LLQEYIKK"
     gene            complement(7417..7842)
                     /locus_tag="PLD15_04920"
     CDS             complement(7417..7842)
                     /locus_tag="PLD15_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63278.1"
                     /translation="MQISLDQFLSAIMARVDNLEATIDDLRLRTNIAMRLVKIMAPDF
                     TRDDVKKAVEDELEITMNAGLMEENERGEEITDRLTDNIYDWLQGDVSELKERMDEYR
                     KKMQEAMEAEKSKVIDVAPAGFVNQLGQERGSKKGKILF"
     gene            complement(7927..8496)
                     /locus_tag="PLD15_04925"
     CDS             complement(7927..8496)
                     /locus_tag="PLD15_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63279.1"
                     /translation="MKLKYSTLAIATAETVIAILAVMSFFLASSSYAGTVLFFSLIIG
                     PLQLRSIVPVDNPGNFRRMLSDIIKRPCTLLLIVLTAVTHGETWYFAILAALCMLSLV
                     FSFAFVSFVLFKRNLAVLESSIAALLGTGLIALGIIDSDFFGGFFWGKNLPSGWIDSV
                     AAGDLMAAALVLVLSAWLIRMRYRSQISR"
     gene            complement(8502..9254)
                     /locus_tag="PLD15_04930"
     CDS             complement(8502..9254)
                     /locus_tag="PLD15_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63280.1"
                     /translation="MIVARGLTRRFGNLIAVNDLNLCVESGEIYGFLGPNGAGKTTTI
                     KMLTGVMEPNSGEIEIAGLRMRESGMEIKRLIAVVPDEPKMYDNLKGIEFAMFIIDIY
                     RLPKRETLKRLYEIAEIFGIDYLGKYISDYSHGMKQKLMVSIALMRNPSVIFLDEPTV
                     GLDAASARKLKLTLRAMAGGGAAIFFTTHVLEVAEKMCDRIGIIDNGRLIAEGTLNEL
                     RSRSGKMEYSLEELFLDMTGNGETAYGNGSGE"
     gene            complement(9251..>10021)
                     /locus_tag="PLD15_04935"
     CDS             complement(9251..>10021)
                     /locus_tag="PLD15_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPB63281.1"
                     /translation="DETTTGFRPSDLVIVAARPSMGKTAFALSIACKMALEFRIPVGI
                     FCLEMSKEQLAQRLLCNVSNFELSKLRAGDINNEEWKRLTTGASVLQTAPIIIDDEPS
                     LDPRTLRAKARRIKMEHSIQVLFVDYLQLMHTKGKTDNRQQEISEISRSMKLLARELK
                     ITVVALSQLSRAVEQREDKTPRLSDLRESGAIEQDADTVMFLYREEYYKDKSEVSDMP
                     QVTKIIIGKQRNGPVGTLEMIFNPKTVAFFEKAFEVER"
BASE COUNT         2502 a         2421 c         2637 g         2461 t
ORIGIN      
        1 tttctgagga ggagtggcag agatcgataa acgaagcctt tcaggacagc aagaaactgg
       61 ggatcgtctc tttcagggga ggtcaacctc ccgaccaccc ggaggtcagt tccatatacg
      121 agacgaagta cctcaaattc cttatataca gggtgtttgg tatcaacgag ttttgatttt
      181 cctttctgtt gcgagctctt gttaatgtac aatgttataa ttcataaaag tctaacggct
      241 ggaggttgat ggaaatggcc aaggtattcg ttgtgacttc cggaaaaggg ggagtcggaa
      301 agacgacagt aacggccaat atcggttgtg ctctcgccaa caagggcgcc aaggtctgtt
      361 taatagacgc agacataggt ctgaagaacc ttgatatcgc cctgggtctg gagaacagag
      421 ttgtgcatac gatactggac gttgcgaacg gaaaagtaac ggcttctgag gctctggtga
      481 ggcacaaaca gataaagggg ctgtaccttc tggcggcatc gcagatagcg accaaggaga
      541 tgctctctcc cgaggacatg aagaaaatcg tggcggagat ttatccgaag ttcgactata
      601 tcatcgtcga ttcccccgcc ggaatcgaaa gaggcttcag aaacgctatc gctgctgccg
      661 aaagggcttt ggtggttacc accccggagc ttccagctat taccgacgca gacagagtca
      721 tagggctcct ggaaaactcc ggtatgctcg aaagcaatat acgccttctc ataaaccgtt
      781 tcaagataca gatggttaag cgcggagaca tgctcaccag ggaagatata cagggaaacc
      841 tcgccataga cctcatcggc atcatccccg acagcgacga agtgatcgtg gcgacaaaca
      901 agggcgtgcc ggttgtcctc aacggaaacg gtgaggggat cggaagggtt ttcaacaaca
      961 tcgcccagag aataaaaggg gaagcgattc cagtcgaaaa cgacatccag gaacagggat
     1021 cccgcggatt cctggaattc ctcaagcgac ttttcagaag aaactgagag ggagttgaag
     1081 cgtatgttct ttggactttt cagaaaaaag aagaagactg aaggaagcag aaaagaagcg
     1141 aaggatagac tggaatcgat caccggcggt agaagatact ccgtgccggt tcgagaagtg
     1201 attcctcaag agcttctcca atccagcggc aaggatatga tctaccagat aaaggcttac
     1261 gtcgccgaca aatacaaggt aaaagaagag aacgtcaagg tccagctcga agagcacaac
     1321 ggttatgttg tgataatcac caacgttgtc ttccactgag ccatggtata tacgataaaa
     1381 agcggtaagc gtgttgaatt catagacatc accgatctgg ttcaccagtc tctcagagaa
     1441 tcacgtgtct cccacggtgt ggtcaacgtg ttcgtacccc acacgactgc cggcgcaacg
     1501 gtaaatgaaa actccgaccc gaacgttgta gaggatatga taaactggct gggtcgtttg
     1561 gtgccggtca gtgaagagtt caggcatttc gaaggaaatt ctgacgccca tatcaaagcc
     1621 acccttgtag gttcttcggt aaatattcca ttcagcgata gtgagctgaa tctcggagtc
     1681 tggcaggccg tatatttttg cgagttcgac ggtccaagga agagaaacgt tgtgatctcg
     1741 gtgatcggat aggagagacg taaaaaagaa tactggtgcg aagggtacat tgaaaggagc
     1801 ctttatgcgg aaaaggtcgc tgcttttcta tgtagttttc atcttcggtt cttccctttt
     1861 tgccataaag ggtttcacct gggacatggg tcttcccaag ttctatttcg cctcggtcat
     1921 gatctctatt ttgatcatat ttctaggtat gaaggccgtt cgctcgccat gggagctctc
     1981 tatggctttt ccgcagatca tcgcgctgat gttggccgct tactcctgtt taaccaccct
     2041 gttacttctt gagagccttc ccgaggtctt tcccgtttca ctgggttacg ccatgaactt
     2101 cctgctcttc gtggtctttt cggttctgat ctctaaggat tccggagaca agctgctgat
     2161 ctttttgcag attttcatcg ttgccggtct cgtgatcgcc ctggacgctt tgttcgcttt
     2221 ctactcggga acgagtttgc tctggggtac ggaaagcgct cccctgtcgc gtggcaatct
     2281 ggcctcggtg ataggcaacg taaacttcac gacggatctg atggggatgt tactcctgcc
     2341 ggtggcattc ttggtcgtta cgaagaagcc cgtttggaag cgtgaaactc ttcgcagagt
     2401 gttttatctg gttgtcttct cggtcctcct tgtggttatt ctaataggtc agacgagatc
     2461 ggtatattat tcggttattg gttctttgat tttcgttgtt ctattctcaa cggtactctt
     2521 gataaggttc cggccgaagg gagttttcac cgcgttcagc cgcctcttcc ttcttttgct
     2581 gttgctggtg acagttgctg tcgtctggac ctattcagga gacaatttcc tcacacaggg
     2641 aagcttcagc ttctcggaaa gattgacgta cactttcgag gacagcattt ccgttgattc
     2701 gcgtcttctc cagtgggaag cggcgcttga gcaatggaag ggatcgaagg tcctcgggac
     2761 cggtttcggc tcctacaagt acttctcgac ggaaaatatg ggtgtggttc tcgccacgaa
     2821 accccagtac atgtacgcgg ccggtctgaa cagcataagg gcgcacaacg agtacacaca
     2881 gatgcttggc gagacgggtg tggtcggtct gtcgctggta tttcttttca tcttttcgat
     2941 ggcgatacac tcctggaagg tggtctccag gacagattct cccaaaaacg ctctcgagta
     3001 cctctttctg gtcgccggtc tgctcatact tttcatagat tcgatccttt catttccagc
     3061 ccatctgatg cctaatgccc ttttaggggt ttttctcttc ggctttgcca tgagaaggga
     3121 atactacggt ggcaggagac taaccgtaaa gttcggtggg gcagtatcgt tgatcgcggt
     3181 cgttttctcg ctggggactt cggtcctgat gagcaggaat ttcatctcgg aaggactttt
     3241 caccaggggt tatatagcct acaaaaacct cgaaaatatc aatccgcaga tcccagggct
     3301 tctggaatca attagcagca taaaggtgga aaaggaactg ttgcagaatt tcacgggtac
     3361 ctacacggcg ctcgcaacag atacctacgt cacctccaga gagaaccggc tgcgtgaaag
     3421 gtatcccgtt gctccggaaa ggctccttca cgatatggcc gttcaggaac aagaactggc
     3481 ttacaaatcg gctcagtctc agctggattc aaggttgagg gcagcttcta gcgctttgat
     3541 gaacgccagg caggacggga acgaaaactt ctacagggct ctcaggaacc tggctctatc
     3601 gagagagctc tcaccagggc aatacatggc cgatgtgtat ttcggctatc tctacctgac
     3661 tgcacagagg aaagaggatt tcaggctcaa gctttccatg agtcagggta atcttgaggc
     3721 agtctacagg gagatcttct caagagagga tgccttctcg gcgtggctta acgattccgg
     3781 tcagaccggc ggtcttctga atgaaatttc gctcgatcac gcgttcttga ggaaccttcc
     3841 ggacgcgata tttcataccg taagctcgac cgaccttcag tcgcttcttg acggactcga
     3901 ttcagatctt ttgatcgatt ttcagctttc tctcgatgcg atcgacgcgc tcttgaggtc
     3961 gcttagcacg gctcccgatc tgcaggtggt gagaaacacc gccagtctcc tcttcagggt
     4021 aatgatcgac tcatctggag tgtctgccga attgaaggag atcgttccga tcctgaaaga
     4081 tcccgccact ttgaacggaa taatcgcaga tcttgatgat ctatcgtctt ctcacaggcg
     4141 tgagatggtc gccctttacg aagcggccat agccaacagc cctggaagct ggctcaaaga
     4201 cgatccgaac atctacgaag agtactcgag aaacctcctc atggtttacg gcgagggcga
     4261 acttgcgaaa gtcctggaga tcgcggagag cgagctcttc gcctggagtt acatgaagag
     4321 ccagaacgtt gtgcccgacg gggttctgaa ggttctccgg tcttttaaag aagatctaaa
     4381 aattggatgg gtagaggctc tgtacggaga ggcggcggcc tggtgcgaag agagaatcgc
     4441 ctttttgaaa ccggagctgg agaaccagga tatcccgaac gtggaaaggg aaaagcttaa
     4501 cgtctttctt agaagagcag acggcttcat aaaattgtac aaatattttc tggaatagtg
     4561 aaaagtcacc aggatgtaac ataagcaacg tatctggcat tgctgacgtt attgatcgga
     4621 cttatgatag ttatagcgat gcctggaggt ataagatgct ggaagtaaag gatataaagc
     4681 tggttcgcga cggaagggcg attctgaaca gcctgtccgc gcagttcgaa aagggaaagg
     4741 tttacgccgt tctcggcaac aacggcgtgg gtaaatcgac gctttcgtac gttttgatgg
     4801 gactggaatc ccaccgcgat tacgacggca gtatagtact tgatggagat cgaataaacg
     4861 gcctgtcggt gcccgaaagg gcaaaaaaag gcattacgct gggctggcag gagccggcca
     4921 gatttaccgg attgaccgtt aaagaatacc tcaccctcgg tggaaagatg aagttatctt
     4981 ccgaagagct gctggaaata ctggcggtgg tggggctgaa cggcgattac ctggatcgtt
     5041 tcgttgacga taccttgagc ggaggcgaga gaaaacgtgt cgaactggcc tccataatga
     5101 tcgtcaaccc tcatgtggtt atactggacg aaccggattc cggtatagat atcatgtcga
     5161 tggagatgat cgaaaacgtt ctcgagaaac tgaaagacaa aggaacgacc gtcataataa
     5221 tcacccaccg cgaagagata gcgcgaatgg ccgatgaggc ttaccttctc tgcggtggaa
     5281 gaatgctcgc atcgggagaa ccagaagaga tagtccagtt ttacagaaag ctctgcgaca
     5341 aatgccagca cataaacgag cccgtcagag gagacgtttc gacatgatcg agtccagtta
     5401 cgaacgtgaa ttcaaagcga tagctcagga atacgaaagg tccggtggta atgtatcgga
     5461 ctttctcaga aaagatatag tatcgataat cgtgagcggc aacaagatta tcggaagaaa
     5521 caccgtcgaa ggagtccatc tcagggcaaa agaactggat aacggtgtcg aagtctggct
     5581 ggatatagac gacgggatcg ttgttgataa ccccatccac ctttgtacgg gatacttaaa
     5641 gcccgagggt gttcagaccg ttctcattca caacaggatc ggcgacggtt cgaaggtgaa
     5701 gttcatctct cattgcgttt tcccgagtgg aaagaacttc acgcattcga tggttgcgga
     5761 tacgaacgtg ggtaaaggtg ccgagatgct gtacgaagat acgcacatgc acagcaaaga
     5821 cggcggcgta acggtaatgg ccacttacag gactagagtc gaagaaggcg gacgttttca
     5881 gaacctcttc tacctcacaa agactcgcgt gggcaagctc tacgttaaaa tgtacgtgga
     5941 tctcgagaaa aacgcttccg cgcacctcga atccaaggtc tatgaaaggg aggacgacta
     6001 tctggagatc gatgaggaga tcaacctcaa cggcgagggc tcttcaggaa tagccaagac
     6061 gacggtcttc gccacagatc agagcaaggc taagatcatc aacaaggcct acggaaatgc
     6121 acctttctcg agaggacata tagaatgcaa cgagataatc aagggagacg ccgtcgaggt
     6181 cggtacggtg ccggaactgt atgtgaagaa cgaaaaggcc gaattgacac acgaggcctc
     6241 catcggcagg gtcaacgtga agcagatgga aacgctcatg tctaaaggtc tcagcgaaga
     6301 agaagcgacc gatttgataa tcaaagggat gctcagataa aaagaacagg gggtaggcgg
     6361 tttgaagtat cttgaagatt acacgatcag gaagatcctg gagctggtga atattcccag
     6421 cccttcggga ttcacggaga aaattatcga gtttgtggca acccagttgc gggagatggg
     6481 tttcgagccg cgtatcacca gaaagggagc gctggtggtc gagctgggcg gttcgggaaa
     6541 tccgctcgtt ctagtggctc acgtcgatac gctgggtgcg atggtgaagt ctctgaaatc
     6601 taacggcaga ctggagataa ccaacgtagg tggtttcacc atgaacagca tagagaacga
     6661 aaactgcacg atccacacga aatcgggaaa gacgtacacc ggaaccatac agagtctggc
     6721 gccgtcggta catgttttcg aaaacgccag aaccctggag cgaaaaatgt caaacatgga
     6781 aataagggtc gatgaagagg tctcctgtca gtccgatctc gaaaagctgg gaatagaggc
     6841 cggggatttc atctctttcc atcccagagc cgtggttacc gacagtggtt tcataaagac
     6901 cagacatctc gatgacaagg caagcgcggc cattttattg ggcctggcca gaaaggtctc
     6961 catcggagag ctggaatgcg gccggaaggt ctacctcttt ttctctaact acgaggaggt
     7021 cggacacggt gcttcggctg cgatgccgga agactcggag gagataatat ctgtcgatat
     7081 gggcgccgtg ggcgacacgc tcaagaccga cgagtatgtg gtttccatct gtgccaaaga
     7141 ttccggcggt ccctacgatg gccagacagt gaaaaaactc atcgatgccg ccagaagggc
     7201 cggtgccgat tacagggtgg atatctatcc cttctatgga tcggatgttg aagcgtcgct
     7261 acgtgccgga tacgatgtca tgttcggact gctggggccg ggggttgagg catcgcacgg
     7321 ctacgagagg actcactgta aagccgttga taacacattg aagttgttgc aagagtacat
     7381 aaaaaaatga ggcgctttac gcgcctcatt tttttatcag aagagtatct tccctttttt
     7441 agaacctctt tcctggccga gttgattgac gaagccggcc ggcgccacat caatgacctt
     7501 gctcttctcg gcttccattg cttcctgcat tttcttgcgg tactcgtcca tcctttcctt
     7561 gagttctgag acatcgccct gaagccagtc gtagatgtta tcggtcaatc tatccgtgat
     7621 ctcttcgcca cgctcgttct cctccatgag cccggcgttc attgtgattt cgagttcatc
     7681 ttcgacggct ttttttacgt cgtccctggt aaaatccggt gccataatct tcaccagccg
     7741 catcgcgatg ttggttctca atctcagatc gtctatcgta gcttcgaggt tgtccaccct
     7801 cgccattatc gcactgagaa attgatcaag agatatctgc atgtttttca actcccttct
     7861 aaattttcct cttatatgat actacctgga agtggtgaga gagttaacac tttgttttac
     7921 gtctaattat cgagagattt gagatctgta tctcatcctg atcagccatg cagataaaac
     7981 caggaccagc gccgccgcca tgagatcgcc ggcggccacc gagtctatcc atccggaggg
     8041 aagattcttt ccccaaaaga atcctccaaa gaagtcgctg tcgataatac ccagagcgat
     8101 gaggcccgtt ccaaggaggg ccgcgatcga cgactcaagg acggccagat ttcttttgaa
     8161 aagaacgaaa gatacgaatg cgaatgaaaa aaccagcgat aacatacaca gggccgccag
     8221 gatcgcgaaa taccaggtct ctccgtgagt gacggccgta agtacaatca gtagcaacgt
     8281 acagggtctt ttgattatat ctgaaagcat ccttctaaag ttgcccgggt tgtcaaccgg
     8341 tacgatcgat ctgagttgca gcggaccgat gatcaacgaa aagaaaagga ccgttccagc
     8401 ataggatgag ctcgccagaa agaaagacat aaccgccaga atcgcaataa ccgtctcagc
     8461 tgtggcaatg gccagagtcg aatatttcaa tttcaaaacc ctcactctcc agacccattg
     8521 ccgtaagcgg tctcaccgtt tccggtcatg tcaaggaaca gctcttctag agagtactcc
     8581 atctttcccg atcgactcct cagctcgttc aatgtaccct ctgctatcag tctgccgttg
     8641 tcgatgattc cgatgcggtc gcacattttc tcggccactt cgagcacatg agtcgtgaaa
     8701 aagatggcgg cacccccgcc ggccatcgcc ctcaacgtca gtttgagttt ccgtgcgctc
     8761 gcggcgtcca gtcccactgt gggctcgtcg aggaatatga ccgatgggtt cctcatcagc
     8821 gcgatagaaa ccatcagttt ctgtttcatt ccgtgagaat agtcgcttat gtacttgccc
     8881 aggtagtcta taccgaagat ctcggcgatc tcgtaaaggc gcttcagggt ctctctcttg
     8941 ggaagcctgt atatatcgat gatgaacatc gcgaattcta ttcctttcag gttgtcgtac
     9001 atcttcggct cgtcgggaac cacggcgatc aatctcttta tctccattcc cgattctctc
     9061 atcctcaaac cggcgatctc tatctcgccg gagttcggtt ccatgactcc ggtgagcatc
     9121 ttaatcgtgg tagttttacc cgctccgttc ggtccgagaa agccgtaaat ctcgccgctc
     9181 tcgacgcaga ggttcaggtc gttgacggcg ataaggttgc cgaacctcct ggtcaatccc
     9241 ctggctacaa tcatcgttca acctcgaagg ccttttcgaa gaaagccacc gtcttcgggt
     9301 tgaaaatcat ttcgagcgtc cccaccggcc cgttccgctg tttgccgatg attatcttcg
     9361 tcacctgcgg catgtcgctc acttcgcttt tgtctttgta atactcttcc ctgtacagga
     9421 acatcacggt atcggcatcc tgctcgatag cgccggattc cctcaggtca ctgagccgcg
     9481 gtgtcttgtc ttctcgctgc tcaacggccc tcgatagctg tgagagggca acgacagtta
     9541 tttttaactc tctggccagg agcttcatcg aacgcgagat ctccgagatt tcctgttgtc
     9601 tgttgtcggt tttgcctttc gtgtgcatca gttgcagata atcaacgaag agtacctgta
     9661 tactgtgctc catctttata cgcctggcct tggccctgag tgtgcgcgga tctagagagg
     9721 gttcgtcgtc tatgattatc ggcgctgttt gcaggacgct ggctcccgtt gtgagcctct
     9781 tccattcttc gttgttgata tcgccggcac gcagcttaga gagctcgaaa ttgctgacgt
     9841 tgcatagaag tctttgagcg agctgttctt tcgacatctc gagacagaag atgcccaccg
     9901 ggatcctgaa ctcaagcgcc attttacagg ctatgctcaa ggcaaaggca gtctttccca
     9961 tagagggacg cgccgcaact atcacaagat cggaaggtct gaaaccggta gtcgtctcgt
    10021 c
//
LOCUS       DAOOLY010000068         9819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_7977_length_9819_cov_5.227059, whole genome shotgun sequence.
ACCESSION   DAOOLY010000068 DAOOLY010000000
VERSION     DAOOLY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7977_length_9819_cov_5.227059"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PLD15_04940"
     CDS             complement(<1..1127)
                     /locus_tag="PLD15_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPB63282.1"
                     /translation="MLKIKIPGIESIFPGEKTEFALKHGDIEVAYSLSGSAGEMSLKA
                     VLRNTGSSSVKIGALKLLTIEEVNEPIYYNNWQSWFPFKAYWEQPVVKPFVQFADSTG
                     SSLFSATPIPGLLRNDVRPSDYFIATDELVAGFLSCSLTHPYFTWNDKEYYIDIWIEL
                     FNKAVGAGEEIEIEELLILHGRYLWEMLEAYAERIKEYNGVSFREFNGVGWCSWYHYF
                     TEIDFKELEKNVALLKEIREREAIPYSLVQLDDGYQKDIGDWLETNEKFPSLDEIASM
                     IKAGGFTAGLWLAPFSVSETSEIFSDHNDWLVRGLDGKPKLAYRNWQKNIYALDVTNP
                     EAAEHLYKTMRRLREAGFDYIKIDFLFAGAIPGERFDRSCTP"
     gene            complement(1289..1768)
                     /locus_tag="PLD15_04945"
     CDS             complement(1289..1768)
                     /locus_tag="PLD15_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HPB63283.1"
                     /translation="MIYRVRERIFSIGDHFTIEDEKGVPHFYVRGRVFSIGNKLSLED
                     MNGNELCFIEQKLLRLLPEYTLYFAGKAAATVKKTLTLLRPKFSISSIYGNFEIEGDP
                     WSHNFSILRDGKLAAEVSKRWFSMSDTYGVDVAGGEEPAFILALVIVIDQTLHDRKN"
     gene            complement(1765..2910)
                     /locus_tag="PLD15_04950"
     CDS             complement(1765..2910)
                     /locus_tag="PLD15_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB63284.1"
                     /translation="MKGFNTRSIHEGEEEKVKDAVTMPIFQTSNFLADDSKYASERSE
                     TFYTRVGNPTIGVLERKLASLFAGTGGVFFSSGMGAITTVFHSFLKHGDNIVVSKNVY
                     GGTQSLLRELPDEGVEVRRFDQSDYGSITGLIDGRTRIVYVESMVNPNLILTDIEAVK
                     AGISSSDALLVVDNTFLSPYNFRPLEFGADVDIQSLTKYINGHSDVVAGFAGFKNPET
                     ESKVRRKMVQFGTNGAPFDAFLVNRGVKTLGPRMEIHNRNALAIANYLKDRPEVLRIS
                     YPGLGRVPDCYGNCPGFGGVVYFEMRDRETAKRFMRSCSIFQEATSLAGVESLVTVPS
                     LTSHASFKESELAEAGISPGGIRLSAGIEDIQDLIEDIKWAIKECVR"
     gene            complement(2907..3917)
                     /locus_tag="PLD15_04955"
     CDS             complement(2907..3917)
                     /locus_tag="PLD15_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB63285.1"
                     /translation="MTKVRLGIVGAGIASRELHLPALRKLADLYEITAVNSRTVEKAE
                     DFARLVGTNPRIFSSYDDMLGSGEIDAVALAVPIFLNPGMIKRAVEAKVPVICEKPVA
                     PNIATAPALLALSREATVYIAENYRHIPVYRKAAEMVWKGKIGKPAVFSWQKWVAFGR
                     DNKYVQTGWRQSPEHIGGFISDGGVHDMAALRAVLGEFLEVSGFSRKNFDFLGAEDTA
                     VFNIRLESGATGEYSVFYGSPVARNRLEIVGDEAMISIDKDMAEITISGSRKERILVN
                     KSDGFVEEFRDFHRVLNGQKNVYGSVLEALKDLAIVEAGLLSSKEKRIVGVGGLLRGD
                     LR"
     gene            complement(3979..5067)
                     /gene="galK"
                     /locus_tag="PLD15_04960"
     CDS             complement(3979..5067)
                     /gene="galK"
                     /locus_tag="PLD15_04960"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPB63286.1"
                     /translation="MKRYFQAPGRINIIGEHTDYNDGYVLPAAIDRYVTLSIERTGRE
                     RIELSSAGREAISFREGEIEPAGNWGDYVKGIFHVLKSEKNVEFGGLSIEIESTVPEG
                     AGLSSSAALEVATITALNEVFGLSLTDEERYRLSQRAENEFVGVKCGIMDQFASVMGR
                     QDSAIFLDTVSMEYEYLPLELGAYSLVVFDSKVHHELLSGGYNSRREEAGKALEILGK
                     RSYRDVSMVDLFTARSKMEDLYYRRAMHVVSENMRVLEVVKILVNSNYENLGRFLVQS
                     HESLAYDYEVSCEEVDFTVDTLRNLNGVSGARMIGGGFGGSVLALCEKSEREKIVEFV
                     RERYFEKFNIAMDAYEVRPSRGAGEIRE"
     gene            complement(5064..6023)
                     /gene="galT"
                     /locus_tag="PLD15_04965"
     CDS             complement(5064..6023)
                     /gene="galT"
                     /locus_tag="PLD15_04965"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPB63287.1"
                     /translation="MQELRFNPLVGEWVIVSSTTNERPTQPVDNCPVCPNSLEFHSGY
                     DLATFDNRFPALSPLIEDVFQPDSRLLRNGIAAGKCEVIMYTSSHGLSVPNMDLAQLE
                     KLVEMWCERYLEISSFRHIKSIFIFENRGKEVGASIQHAHGQLYSFPFIPRRLELKAN
                     SFTDYQRANRSCLVCDLLKDESYSRNTIFETAGFTAVVPYFSRFPYEVHLYPKRHVAS
                     VIDLIASEKRDLAYSIRHTVRLYDSIYNQEFPYMMTLYNPPVNTGRIYDDSYHFHIEF
                     YPPKRAKDKLKWMASVETGTWAFVNPAEPEEIADFLRKVEVKI"
     gene            complement(6131..6370)
                     /locus_tag="PLD15_04970"
     CDS             complement(6131..6370)
                     /locus_tag="PLD15_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPB63288.1"
                     /translation="MEAKVVVYSTPTCPWCKRAKDYFKNNGISFKDYDVSKDKAKADE
                     MVRKSGQMGVPVITIGSHVIVGFDKNKIDSLLGTH"
     gene            complement(6474..8075)
                     /locus_tag="PLD15_04975"
     CDS             complement(6474..8075)
                     /locus_tag="PLD15_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPB63289.1"
                     /translation="MKRRFQRKDFFIIFMIVSGFFMFVAMGNGYWRENAPYKISSVDI
                     KASLDENGFLNVAETSLYELSRNNRTASKRLHLSYPAVLESFNVQVDQMTGVVREVSS
                     SPGGFDVRIFVNEDVGSFTAGTNYRLSGVVENGSDVSVLSYRFWEPNSDAMTRNISLS
                     LELPEQIARAITTESIFLKPYKEAKVTIEGNRVLLSMKSVLSSASAEIMVVFPRSIGN
                     NMSRAINTTISRSSIEKENETSMFQQAFLSGLIGFQIAVPIFVLFFTFVLFGIEPQIK
                     TEIGHRIDSAPGYLLNAIVKNPFQEVDRDGFLATILELHNSGEVKLQDRTILASAKNN
                     TMPTQQQWAIQAIKSLGSDGKDEVEIPDQEYKGEGIEAFNRHYGFWQKHAMKVVRSGR
                     FYEHLGSTVMSVFSIFFLITWSLILKYLFANWQVYLTFPDETLVLSIVLYADWCLGWF
                     LLMIPKSIFGRWTPSGRLFYMEWKTAEKEILAKQEFSDEDLEKLVALGYLQTLLADRK
                     RLSERQLGLLRTLQRLELLLTRPKE"
     gene            8139..8927
                     /locus_tag="PLD15_04980"
     CDS             8139..8927
                     /locus_tag="PLD15_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPB63290.1"
                     /translation="MLKGKKLFVSDMDGTFYLGDKLLPGSLDFAIKIRDVGARLVFLT
                     NNSSRTPDVYIRKLIKMGVNRELFEVYTSGEATVRFLMEKYPGRRVYLLSTPSVREMF
                     HRAGIDLVEEDPEVVVLTYDTTIDFGKIRKTALFLREGAVYVASHPDINCPTEDGPVP
                     DVGSFISLFESSTGRKPDWIIGKPSPTILQMLLEDYSQTADGCVIIGDRLYTDIECGL
                     RAGVDAILVLSGETTPEMVSPDHPYIIANDVGEIAKLISQEFAV"
     gene            8951..9514
                     /locus_tag="PLD15_04985"
     CDS             8951..9514
                     /locus_tag="PLD15_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB63291.1"
                     /translation="MVILFIVLGIVVVLALWLIGVYNNLVTLKKRVENAWSQIDVQLK
                     RRHDLIPNLVNSVKGYMKFEQDTLEKVMQARARAVSAQGVGDKIKAEQELGGVLGRLL
                     AIAENYPDLKANTNVSQLMEELTSTENKISYARQFYNDSATKFNTKIEIFPNNIVVGF
                     FGGKFEAFPLFEVGEVERETPNVDLSF"
     gene            9520..>9819
                     /locus_tag="PLD15_04990"
     CDS             9520..>9819
                     /locus_tag="PLD15_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HPB63292.1"
                     /translation="MAFTVDFYEQGRKNVRKTIILVMVFLVMMAAFGLIIDIIFGILP
                     IFTLIFLVVALVQLLISLSSGKEIVLKSVRAREARQDDPEERQLKNIVDELSLAAG"
BASE COUNT         2258 a         2612 c         2442 g         2507 t
ORIGIN      
        1 ggtgtgcatg agcggtcgaa cctttctccc gggatcgccc cggcgaacag aaaatcgatc
       61 tttatgtaat cgaacccggc ctccctcaag cgtctcatgg tcttgtaaag atgttcggcg
      121 gcttcggggt tggtgacgtc cagcgcgtag atattcttct gccagtttct gtaagccagc
      181 ttcggttttc catcaagtcc acggacgagc cagtcattgt ggtcggaaaa gatttccgat
      241 gtctccgaaa cgctgaaggg cgccagccag agaccggcgg tgaagccgcc cgcctttatc
      301 atggaggcga tctcgtcgag cgaagggaac ttctcgttgg tttcgagcca gtctccgata
      361 tctttctggt agccgtcgtc cagctgaacc agggaataag gtatggcctc tctttctcta
      421 atctctttga ggagagcgac gtttttctcg agttctttga agtctatttc cgtgaagtaa
      481 tggtaccagc tgcaccaccc gacgccgttg aattccctga agctcacgcc gttgtattct
      541 tttatcctct ccgcgtaggc ctcaagcatt tcccagaggt atcttccgtg aagaatcaac
      601 agttcctcga tctctatttc ttcacctgca ccgaccgcct tgttgaagag ctctatccag
      661 atgtctatat agtactcttt gtcgttccag gtgaaatagg gatgggtgag cgagcaggaa
      721 agaaaaccgg cgaccagctc gtcggtagcg atgaagtaat cgctgggtct cacatcgttt
      781 ctcagaagcc cgggtatggg agtcgccgaa aagaggctgc tgcctgtcga gtcggcgaac
      841 tggacaaagg gcttcaccac cggttgttcc cagtacgctt tgaaggggaa ccacgactgc
      901 cagttgttgt agtagatcgg ctcgttgact tcttcaatgg tcagaagctt cagcgcaccg
      961 atcttcacgc tcgaagatcc ggtatttctc aaaacggctt tgagcgacat ttcgcctgcc
     1021 gatcccgata gcgaataagc tacctcgata tctccgtgct tgagggcgaa ctccgttttc
     1081 tcgccgggaa atatgctttc tataccggga atttttatct ttaacaactc tcaattcctc
     1141 ccttccggct ttggtgacaa ttataggcta gagtcatttt ccattgaact gtatctacag
     1201 cctctgagag ttcaagagac gcggttaaag gctccgatcg gggccgtgaa agacaatact
     1261 ttcttcagcg tcgattatcc ggcctctatc agttcttcct gtcgtgaaga gtctgatcga
     1321 tcactataac cagggccagt atgaaggcgg gctcttcacc cccggccaca tcgacgccgt
     1381 aggtgtcgct catagaaaac caccgtttgg agacctcggc cgccagtttc ccgtccctga
     1441 gtatgctgaa attgtgtgac cagggatcgc cttcgatctc gaagttgccg tagatactgc
     1501 ttattgagaa cttaggcctt aaaagggtga gggtcttttt cactgttgcc gcggcctttc
     1561 ccgcgaaata cagtgtgtac tcgggcagta atctcagcaa tttctgctcg atgaagcaga
     1621 gttcgttgcc gttcatatct tccagcgaga gcttgttgcc tatagaaaag actcttcccc
     1681 tcacgtagaa atgtggaact cccttttcat cttcaatggt gaaatggtcg ccgatcgaaa
     1741 agatcctctc cctgaccctg tatatcatcg tacacactcc tttatcgccc attttatatc
     1801 ctctataagg tcctgtatgt cttcgatccc tgccgagagc cggataccgc cgggagagat
     1861 accggcctcc gccagctcgg attctttgaa ggaggcgtga ctggtgagcg aggggacggt
     1921 aacgagagat tcaaccccgg ccagactggt ggcttcctgg aagatcgaac aactcctcat
     1981 gaatctcttc gcggtctccc tatctctcat ctcgaaatag acgacccctc caaaccccgg
     2041 gcagttcccg taacagtccg gcacacggcc caggccggga taggaaatcc tgagaacctc
     2101 gggcctgtcc ttcaggtaat tggcgatggc cagcgcgttt ctgttgtgga tctccatcct
     2161 cgggcccagt gtcttcacgc cgcggttgac cagaaaggcg tcgaaggggg ctccgttagt
     2221 tccgaactgg accatctttc ttcgcacctt cgactccgtc tcgggattct tgaagccggc
     2281 gaatccggcc acaacgtcgg aatggccgtt gatatacttg gtgaggctct gaatatccac
     2341 atcggccccg aactccagag gtctgaaatt gtagggagag aggaaggtgt tgtcaaccac
     2401 cagaagggca tcgcttgagg agatcccggc cttcacggcc tcgatgtcgg tgagaataag
     2461 gttaggattg accatcgact cgacataaac gatcctggtt ctcccatcga tcagacccgt
     2521 tatcgatccg taatcggact gatcgaacct cctcacttcc acgccctcgt ccggcaactc
     2581 cctgagcagg ctctgcgtgc cgccgtaaac atttttcgaa acgacgatgt tgtcgccgtg
     2641 tttgagaaag gaatggaaga ccgtcgtgat ggcccccatg cccgacgaga agaaaacgcc
     2701 gcccgtgccc gcgaagagcg aggccagttt gcgctccaga acgcctatgg tagggtttcc
     2761 cacacgggtg taaaatgtct cggatctttc gctggcgtac ttggaatcgt cggccaggaa
     2821 gttggaagtc tggaagatcg gcatggttac ggcatccttc actttctcct cttctccctc
     2881 atgaatcgag cgcgtgttga atcccttcat cgtagatcac ctctcagcag cccgcctacg
     2941 ccgactattc ttttttcctt cgatgacagc agtcccgctt ccactatcgc gagatctttg
     3001 agagcctcca gcacgcttcc ataaacgttc ttctggccgt tgagtacgcg gtgaaaatcc
     3061 ctgaactcct caacgaaacc gtcggatttg ttgactagaa tcctctcctt gcgactgccg
     3121 gaaatcgtga tctcggccat gtctttgtct atcgagatca tcgcctcatc gcctactatc
     3181 tccagccgat tcctggcgac cggagagcca tagaagaccg aatactctcc ggtggctcca
     3241 ctctccagtc tgatgttgaa aacggcggtg tcctccgctc ccagaaaatc gaagttcttc
     3301 ctcgagaagc cgctcacttc gagaaactct cccagcaccg ctctcaaggc ggccatatcg
     3361 tgcacaccgc catcggagat gaaaccgcct atatgttcgg gcgactggcg ccacccggtc
     3421 tggacgtact tgttgtccct tccgaaagct acccatttct gccagctgaa aacggccggc
     3481 ttaccgattt ttcccttcca gaccatctca gcggcctttc tgtaaacggg gatgtgcctg
     3541 tagttttcgg ctatgtacac cgtcgcctcc ctggaaagcg cgagcagcgc gggcgccgtc
     3601 gctatgttag gagcgacggg tttttcacat atgacgggaa ctttcgcctc cacggctctc
     3661 tttatcattc ccgggttcaa aaatatcggc acggccaacg ccacggcgtc tatctccccc
     3721 gaacccagca tatcgtcgta actgctgaat atccgtgggt tggttccgac gagccgggca
     3781 aaatcctcgg ccttttccac ggttctgctg ttcacggccg ttatttcgta cagatctgcg
     3841 agttttcgca gggcaggcag atgtaactcc ctggaggcaa tccccgcccc tacaattcca
     3901 agtctcacct tagtcacact tacctcccgc actcggccct gagattgccc tgacgtgccg
     3961 ttgcatttct cagagggctt attctcttat ctctcccgct ccgcgggagg gcctgacttc
     4021 gtaagcatcc atcgcgatat tgaacttctc gaagtatctc tccctgacaa actcgacgat
     4081 cttctcccgc tcgctctttt cgcataaggc cagtacggaa ccgccgaacc cccctcctat
     4141 cattctggcg ccggagacac cgttcagatt tctgagagta tcgactgtga aatcgacctc
     4201 ctcgcaagag acttcgtaat cgtacgcgag cgactcgtgg gattgaacca ggaacctgcc
     4261 aaggttttcg tagttcgaat tgacgagtat cttgaccact tcgagtactc tcatgttctc
     4321 ggagaccacg tgcatggctc tccggtaata aagatcttcc atttttgatc tggcggtgaa
     4381 aagatcgacc atcgatacat cccgatagct cctcttgccg agtatttcca gggcctttcc
     4441 ggcctcctcc cggcgcgagt tgtacccgcc gctcagaagt tcgtgatgta ccttcgaatc
     4501 gaatacaacc agtgaataag cgcccagttc cagcggaagg tactcgtact ccatgctgac
     4561 cgtatccaga aagatcgcgc tgtcctgcct tcccatcacg ctcgcgaatt gatccatgat
     4621 gccgcacttg acgccaacga attcattctc ggccctctgc gataacctgt acctttcctc
     4681 atcggtgaga gagagtccga aaacttcgtt cagggccgtt atggtggcga cttcaagggc
     4741 ggccgaactc gacagcccgg ctccctcagg cacagtcgat tcgatctcga tcgaaagccc
     4801 tccgaactcc acgttcttct cgctcttgag aacgtggaag attcctttca cgtaatctcc
     4861 ccaatttccg gccggttcga tctctccctc tctgaacgag atcgcttctc taccggcaga
     4921 actcagttcg atacgctccc tccccgtcct ctctatcgaa agggtgacgt acctgtcgat
     4981 cgcggccggc aaaacgtaac cgtcgttgta atcggtgtgt tctcctatga tgtttattct
     5041 tccaggggcc tggaaataac gcttcaaatc ttcacctcca cctttctaag aaaatcagca
     5101 atctcttccg gttcggcagg attgacgaac gcccaggtgc cggtttcgac actcgccatc
     5161 catttgagtt tatctttggc tcttttaggc ggatagaatt ctatgtggaa atggtaagaa
     5221 tcgtcgtaga tccgtcccgt gttcaccggc ggattgtaca gggtcatcat gtacgggaac
     5281 tcctggttgt atatcgagtc gtagagtctg acggtgtgcc ttatcgagta agccagatcc
     5341 cttttctccg aggcgatcag atcgatcacg gaggcaacgt gtcgctttgg atagaggtgg
     5401 acctcgtagg ggaatctgga aaagtacgga acgaccgccg tgaacccggc cgtctcgaag
     5461 atggtgttgc ggctataact ctcatccttg aggagatcgc agaccaggca ggaacggttc
     5521 gcgcgttggt aatccgtgaa agagttggcc ttgagttcaa gacgtctggg aatgaacggg
     5581 aaagagtaca gctggccgtg cgcatgctgg atagaagccc ccacttcctt acccctgttc
     5641 tcgaagatga aaatcgattt gatatgccga aaggaggaga tttccagata gcgttcgcac
     5701 cacatctcca cgagcttttc cagttgagcg agatccatgt tggggacaga taagccgtgg
     5761 gaggaagtgt acataatcac ctcgcacttt ccggcggcga ttccgttcct gagaagcctc
     5821 gaatcgggct ggaaaacatc ttcgatcagc ggggagagtg ccggaaagcg gttgtcgaaa
     5881 gtggcgagat catatccgct gtggaactca agcgagtttg gacagactgg acagttatct
     5941 acaggttgag taggtctttc gttggtggtg gaagatacga taacccattc cccaaccaga
     6001 ggattgaatc tgagttcctg catcttaatc ccccttttgg tgtatcttta ttgacagaat
     6061 aacaccgcgg ttcaaaaaaa taaagcggac tcaacgtccg cctgtttcaa ttataaaggt
     6121 aaaggctgta tcaatgagtt ccgagaagac tgtctatttt gtttttatcg aaccccacta
     6181 tcacgtggct tcctatggtg attaccggaa cacccatctg tccgctcttt ctgaccatct
     6241 cgtctgcctt tgctttatcc ttgctcacat cgtagtcttt gaaggaaatc ccgttgttct
     6301 tgaaataatc tttcgctctc ttgcaccagg gacatgtggg agtagaataa acaactactt
     6361 tcgcttccat attttctcgc ctccatatac cccaccaggg tattattcat aaatattata
     6421 gaataatcgg gtgggtcgtg tcaagtgctt acagaaatca actggaaggc gcgtcattcc
     6481 ttcggtctag tcagaagcaa ctcgagtctc tggagtgttc gcaaaagccc gagctgtctt
     6541 tcactcaatc tctttctatc ggccaggaga gtttgcaaat aacccagggc cacgagcttt
     6601 tcgagatctt cgtccgagaa ctcctgtttg gccagaatct ctttctccgc ggtcttccac
     6661 tccatataga acagccggcc cgagggcgtc cagcggccga agatactctt tggtatcatc
     6721 agcagaaacc atccgagaca ccaatcggcg tacagaacga tcgaaagcac gagcgtctcg
     6781 tcggggaagg tcaggtaaac ctgccagttc gcgaagaggt atttcagtat cagcgaccag
     6841 gttatcagga agaatatcga gaagaccgac ataaccgtgg agccgaggtg ttcgtaaaac
     6901 ctccccgaac ggacgacctt catggcgtgt ttctgccaga agccgtagtg tctgttaaag
     6961 gcttcgatcc cttcgccttt gtattcctgg tcgggtatct cgacctcgtc ctttccgtcg
     7021 ctgccgagag atttgatcgc ctgtatcgcc cactgctgtt gagttggcat ggtgttgttc
     7081 ttggcgctgg ccagtatagt tctatcctgg agcttgacct cgccgctgtt gtgcaattcg
     7141 agtatcgtgg cgaggaaacc gtctctgtcg acctcctgaa agggattctt cacgattgcg
     7201 ttcagcaggt aaccgggagc cgagtcgatt ctgtggccta tctcggtctt tatctgcggc
     7261 tctatcccga agaggacgaa tgtgaagaac aggacaaaga tcggaacggc tatttgaaag
     7321 ccaatcagac cggagagaaa ggcctgctgg aacatgctgg tctcgttttc cttttcgatc
     7381 gaactcctgc tgatggtagt attgatggcc cttgacatgt tgtttcctat actccttgga
     7441 aagaccacca ttatctcggc agaagcgctc gaaagaacgg atttcatgct cagcaatacc
     7501 ctgttgcctt ctatggtaac tttcgcttcc ttgtagggtt taagaaagat gctttcggtc
     7561 gtgatcgccc tggcgatctg ttcgggtagt tccagagaga gtgaaatatt tctggtcata
     7621 gcgtcggaat tcggctccca gaaacgatag ctgaggacag agacgtcgct gccgttttca
     7681 actactccag aaagcctgta gttcgtgccg gcggtaaaac ttcccacatc ttcgttcacg
     7741 aatattctca catcgaaacc gcccggcgag gaggatacct ccctgactac accggtcatc
     7801 tgatcgacct gtacgttgaa agattcgaga acggccgggt aagaaaggtg aagtctcttc
     7861 gaagcggtgc ggttgtttct ggaaagctcg tacagcgatg tttctgccac gttcaggaaa
     7921 ccgttttcat ctagagacgc ctttatatcg acggagctta tcttgtaagg agcattttcc
     7981 ctccagtaac cgttccccat cgcgacgaac atgaagaaac cgctcactat catgaaaatg
     8041 atgaaaaaat cctttctctg gaatctcctc ttcaatttat ctcaccctct gcattatact
     8101 cttaatatag ttatgtcaac tgaaccccgg aggtagagat gctaaaaggc aaaaagctgt
     8161 tcgtttccga tatggatgga accttttacc tgggcgataa gctgctgccc ggtagtctag
     8221 attttgccat aaaaatacgt gatgtgggtg ccaggctcgt gtttctcacg aacaattcct
     8281 cacgtacacc tgatgtatat atcagaaaac tgatcaaaat gggagtgaac agagagttgt
     8341 tcgaagtcta tacatcgggc gaggctaccg tcagattctt gatggaaaag tatcccggca
     8401 gacgcgtata tctcctttca actccatcgg tcagagagat gtttcacagg gccggcatag
     8461 atctggtgga agaggatccc gaagtggttg ttctaactta cgacaccacc atagatttcg
     8521 gcaagatcag aaaaacggcc cttttcctca gagagggagc cgtgtatgtg gccagtcacc
     8581 cggacataaa ctgcccgaca gaggatggtc ctgttcccga tgtaggcagt tttataagtc
     8641 tcttcgagag ttcgaccgga agaaagccgg actggataat cggaaagcca agtcccacga
     8701 ttctccagat gctgcttgag gactattctc agaccgccga cgggtgcgtt ataatcggag
     8761 accgcctcta caccgatata gaatgcggtt tgagggccgg ggttgatgca attctggtac
     8821 tttccggaga aactacgccc gaaatggttt ctccagatca tccttacatt attgcaaatg
     8881 atgtggggga gattgcaaag ctgattagtc aggagtttgc tgtataattc tgatagctgg
     8941 aggtgattga atggtcattc tttttatcgt actaggtata gttgttgttc tcgctctttg
     9001 gctaataggg gtttacaaca acctggtcac cctgaagaag agagtcgaga acgcatggtc
     9061 tcagatcgat gttcagctca agagaagaca tgacttgatt ccaaaccttg taaattccgt
     9121 aaagggatac atgaaattcg aacaggatac gctggagaaa gtgatgcagg ccagggcccg
     9181 tgcagtatcg gcccagggtg tgggagacaa gataaaggcc gagcaggaac tgggcggggt
     9241 gctcggaaga ttgctcgcga ttgccgagaa ttatcccgat ctcaaggcca acaccaacgt
     9301 ctctcaactc atggaggaat tgaccagcac cgagaacaag atatcctatg ccagacagtt
     9361 ctacaacgat tcggcgacta aattcaatac caagatcgaa attttcccca acaacatagt
     9421 ggtgggtttc ttcggcggaa agttcgaagc cttcccgctt ttcgaagtcg gtgaagtcga
     9481 gagagaaact cccaacgtag atctttcgtt ctgagggata tggcctttac agtagatttc
     9541 tacgaacagg gcagaaaaaa tgtgagaaag accatcattc tcgtgatggt ctttcttgtc
     9601 atgatggccg cttttggact gataatcgat ataatcttcg gaatactgcc gatcttcacc
     9661 ctgatctttc tggtggtcgc tctggtgcaa ctcctgatat cactgagctc gggaaaggag
     9721 atagttctca agtccgttag ggccagagaa gcgcggcagg acgatccaga agagagacaa
     9781 ctgaaaaaca tagtcgatga gctgtctctg gcggccggg
//
LOCUS       DAOOLY010000069         9525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8363_length_9525_cov_4.999050, whole genome shotgun sequence.
ACCESSION   DAOOLY010000069 DAOOLY010000000
VERSION     DAOOLY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8363_length_9525_cov_4.999050"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PLD15_04995"
     CDS             complement(<1..1213)
                     /locus_tag="PLD15_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPB63293.1"
                     /translation="MKKTLLLISVVLLALVFASCMRVAPGNSAGANVEFVWKQIQDAP
                     YVEGRLIVGYSDIEALSEIVSLLDAEITIDIPRLNAAGLRFDSTVEEARGAIRELFVE
                     RPELVASIRYIEPNYERELIKPVKDDNLKLPELPVVLAEDTFPDLEGFLWGIKKVRAP
                     QAWAAGYDGTGIIVAVLDTGIDSTHPDLQGQITHRYDPFLDLEIDPDIDYSFEAHGSH
                     VAGTIAAKDDGSGVIGVAPGAKIMDIPIFQPNYIGDEFVAAGIVYAIENGATVLSNSW
                     GGKGFSTLLYDAIAYASENYVVFVNSAGNEHVNEVKSPAMYPGVVVVAASTAGDGITH
                     FSSRSVKLSVAAPGDFSILSTVPLWDVAEFAYPDLPYAYYGGTSMACPHVSGAIAILQ
                     QKYKDMGLTVYQ"
     gene            complement(1231..3201)
                     /locus_tag="PLD15_05000"
     CDS             complement(1231..3201)
                     /locus_tag="PLD15_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63294.1"
                     /translation="MKKSILLLVVIVAAMLVVTSCIKPLKPTEATLEITSIVVSGNEA
                     TISWKLNTTAEIVSASYTLHVGEAEYESQETSKTVTLAAGTHQVKVEGEIKGKYEATY
                     GQPAVHVESETETVEIMQEYGNTLLINLVEYMTGPAAAGVSLEVFDSETGALLGSGET
                     DLEGKAEIDLLWFLSPGLLEVREVTVKAERTGLAPSVLEGLAVPLIPSNELASAGSPL
                     PVDMAINRAMIDPLDGSYPEIEITLLDGNGNEIDPTDVSTNVYLNIEYASDLEYDVWD
                     VLYVGVGYVPWAGVRDFASFGMSKAAFAIPAEIFNGDLPVHVVVYNYNRARLDRVVNL
                     NFTAEKAGPAETSPPTGVWLWSWTSDANVNYYSLPKEINGRKLNLDEETFENLMGAPA
                     EGNIFVQIAWDLPENLAGLVGFNIYRSSDGVNYKKIAFRTALYGFDSGIGIEAGNRYF
                     YKVRSVYADGSESEDSNIASVVPLDIFKVKLLSPANNSTGVSRRPVLKWAPVDKVNSN
                     KVPVLGGGLLDESEITYHYTPWIYDTVQSDYQHLLAVDSEFETFDFSTYGPQQVSVKF
                     LDPFWLGKVMNWIFILNGQAYLYPFDQLEAFKTYEWGLDYAVATYYGYDEDGDGFYDS
                     VAYSTTIDLGYDFDRWVNDADYYNRFTTGGDN"
     gene            3400..4884
                     /locus_tag="PLD15_05005"
     CDS             3400..4884
                     /locus_tag="PLD15_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63295.1"
                     /translation="MKRISFMLVLLTALVLLVTSCFKLPFVVVTLSSDAGSNTVLAGT
                     TITFSWKFETADDFKEVVYKLHINDDILELETTEYATELVAGNYKVWVEALGKLAIES
                     NFRTFNSNELEFTVGSPSVIFDNTQAAYASKDVTVSWKPVDGLTHTYLYSVDGAAFVE
                     TDALKVDLKSLADGTHFVEVKTKEFNGLPSKYVFKVDTTGPSITIFGSDRYNGGLGDG
                     DFRPFGRYIFASWQFSEPAVKVEIRFRETTEAGYPWITDWLDWDPAVSGILLNDELVT
                     LYLTEGTFMMPYMLELGHTYLLYVRAYDELGNNKNTYVSFKLEDRYTGEDKPEIFIAP
                     ITAQPATDEEDGSLVIAVVAPNVKEYIDKIKEYFAPDNTRNGELMYLQNSLLYSQGLT
                     VTQVFFPDFIPDKKNVSNFVDFGDELVLVRGFVNEPAEIEEAGDVVAFVEFVFGPEMN
                     GQEVSLGLGTDYFIRDGANRTIDGIVVDNFIYGLDLPEIVGAEM"
     gene            4897..6345
                     /locus_tag="PLD15_05010"
     CDS             4897..6345
                     /locus_tag="PLD15_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPB63296.1"
                     /translation="MKKTLLVIIITLVSIATFGNELLQEAGSRAAEVNESIARLNLPW
                     RAGVPGALSEFAAHGIVDLDSIIEKWHGEVELPERMKMDLTKSLFVDQATTRYSQLIS
                     ALFVYFPYGDVHIPEGSFVQVHTPITNQAFHGTCWAFATVASFESGLLVQKDGLAGEE
                     ITEPWKLQHDTYDLSEQFASFHNIDWDIYLESIYDPLQSDVIIQDDSSDAGGNAIFST
                     YNSVRYGLPLESDFPYSMFDFNPWILWNPVNNNWEENIVKSTKTVQVYYGDELSWLGY
                     PYEFYNGAIKEAMIKFGSLAVSFRVPADFDYYTEGVYIPTSTTITGGHAVTLVGWLDM
                     AGLKAAFPDLIGPEATSITVNDPFSGLTWEATEFWIIKNSWGDWGWNGYYVVPAVSKQ
                     LYEFSANNGFGISPWMIEYNAMYVPIFEETYAMEDVDFNGDGVVDEKDFDILVNHMYT
                     ESIVHDISIPKDYWVDHEDISRFLLIWNSIID"
     gene            6552..7796
                     /locus_tag="PLD15_05015"
     CDS             6552..7796
                     /locus_tag="PLD15_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63297.1"
                     /translation="MRRLASLLLVVFLIFLLSSCFKQLNVSVEFENLQTEYATKNVTV
                     SWKPTGGVDHLYLYSIDGDGYVETSETELELTNLEEGQHTVEVKIAGSTRIPDKFTFI
                     VDTEGPLVRFMGSDRYSYGLFSEDFTPFGRYVFLKWEFSEEISKVEFRFVDTNSEEFQ
                     YITDWLEWNPASTSMLISDEGLTANLKEGNTVLDVFEVGKIYRMYIRAYDKLGNTRYS
                     DQYRPFVVFRLDERYVDEDKPLVYLEPGQILPAEGENRGSIEVAIVASNVAKYVESVK
                     EYFAPDNTRNGELMYLQFSVVHDEGLVFEEAFFPQFIENKTSFDGVIPSNREAVVVRG
                     FIDEAPLIPAVSDIVAILRFSFPQELSGESVSFKIGSRPKDALSGPMYFIRDEANRVI
                     DGITVDNQFVEVSLPELVAAGM"
     gene            7807..9279
                     /locus_tag="PLD15_05020"
     CDS             7807..9279
                     /locus_tag="PLD15_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPB63298.1"
                     /translation="MRKTLIILALVLLACASFAGTLLETAAVRSAEVERAISELGLPW
                     RAGVPAILKEYAANGVSDLDSLIDKWNGTLELLEPIGAPDAQDFEVQATTRYNQLLSA
                     MVNYDYCGDLFVPEGGFIQVHPAVRNQGAHGTCWAFATVASFESALLVQEDRLPGGWS
                     YAPWEYQYDLYNLSEQFLAFHNVDDDIYINSLRDPLQSDVIIQDSNEDTGGWPIFSTY
                     NCVRYGLPLDSDFPYIKYDRKNWIVWNPTNNYWEDNIIRSTKTIRIYGGSTLMSFLPY
                     DEYVMAIKELLVNYGALVTSFEVPVDFSYYKEGVYVPSTATIRGGHAVTLVGWLDMDG
                     LKAAFPDLINPQATSITIEDPFIEEQLFDLDEYKWEATEFWVIKNSWSEDWGWKGYYV
                     VPMISEELYNFLIEEFYALPKWMIECDDMYVPLFEESYETSEDIDFNGDGIVDRADFE
                     LLLANMFTTSEKYDISIPKDGRVDQEDASRFFVIFNSMLD"
     gene            complement(9327..>9525)
                     /locus_tag="PLD15_05025"
     CDS             complement(9327..>9525)
                     /locus_tag="PLD15_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63299.1"
                     /translation="ASPGHYSYSVVPYGGMEGFFEGKDVLMAGYVEYDNDPGRRIAFK
                     VLMSEGIGIIADGHSPLFSAF"
BASE COUNT         2296 a         2536 c         2477 g         2216 t
ORIGIN      
        1 actggtaaac tgtcagtccc atatccttgt atttctgctg gagaatggcg atcgcacccg
       61 agacgtgcgg gcaggccatc gacgtcccgc cgtaatatgc gtagggaaga tcgggatagg
      121 caaactcggc cacatcccac aacggaacgg tcgaaaggat cgagaaatcg ccgggagcgg
      181 ccaccgagag cttgacgctc cggctcgaga aatgggtgat cccgtcgccc gctgtggagg
      241 cggccacgac cacgactccc gggtacatgg caggactctt gacttcgttc acatgttcat
      301 ttccggccga gttcacgaag accacatagt tctcggaggc gtaagcgatc gcatcgtaca
      361 gaagcgtcga aaagcccttg cctccccagg agttcgaaag taccgtggcc ccattttcga
      421 tcgcatatac tatgccggct gcgacaaact cgtctcctat atagttgggc tggaagatcg
      481 gtatatccat tatcttcgct ccgggggcca cgcctatgac accgctgcca tcgtcctttg
      541 cggcgatagt accggcgacg tgagatccgt gcgcttcgaa ggagtagtct atgtcggggt
      601 cgatctccag atcgaggaac ggatcgtacc tgtgggttat ctgaccctgg agatctggat
      661 gggtcgagtc gatgcccgta tcaaggacgg cgacgattat gccggtcccg tcgtacccgg
      721 cggcccaggc ctgcggtgca cggaccttct tgattcccca gaggaatcct tcaaggtcgg
      781 ggaacgtatc ttcggcaaga accacgggca gttcgggcaa tttcagatta tcgtctttca
      841 ccggctttat aagttccctt tcgtagttcg gttctatgta ccttatcgat gcgacgagct
      901 ccggtctctc gacaaaaagc tccctgatag cgccccttgc ctcttcgacc gtcgagtcga
      961 accgcaaacc cgcagcgttc aagcgcggta tatcgatggt tatctcggca tcgagaagag
     1021 aaacgatctc gctcaaagcc tcgatatcgc tgtaacctac tatgagtctt ccttcaacgt
     1081 agggcgcatc ctgtatctgc ttccatacga actccacatt ggcgccggcg ctgttacccg
     1141 gggcaactct catacaggaa gcgaacacaa gggccagcaa tacgacactt attaacagta
     1201 aagtcttctt catctgcacc acctcccctc tcaattatct ccaccggtgg tgaagcggtt
     1261 gtagtaatcg gcatcgttga cccacctgtc gaaatcgtag cccaggtcta tagtggtcga
     1321 ataagcgacc gaatcgtaga agccgtctcc gtcttcatca tatccgtagt aggtggccac
     1381 cgcgtaatcc agaccccact cataggtctt gaaggcctcg agctgatcga aggggtacag
     1441 gtacgcctga ccgttgagaa tgaagatcca gttcatcact ttcccaagcc agaacggatc
     1501 cagaaacttc acggagacct gttgtgggcc gtaagtggag aaatcgaagg tttcgaattc
     1561 gctgtcgact gccagcagat gctggtagtc gctctgaaca gtgtcgtata tccagggcgt
     1621 atagtgatag gtgatctcgc tctcgtcgag gagaccgccg cccagtacag gcactttgtt
     1681 agaattgact ttgtcgacgg gcgcccactt cagtaccggt cttctcgaaa ctcccgtcga
     1741 gttgttcgcc ggggagagca gcttgacctt gaagatatcg agcggcacta ccgaggcgat
     1801 gttgctgtcc tccgactcgc ttccgtcggc gtaaaccgat ctgaccttgt agaaatacct
     1861 gttgcctgcc tcgatgccga ttccgctatc gaaaccgtaa agggcggtcc tgaaagctat
     1921 cttcttgtaa ttgacaccgt cactggagcg gtagatgttg aaacccacta accccgcaag
     1981 gttctcgggg aggtcccagg cgatctgaac gaatatgttg ccctctgccg gggctcccat
     2041 gagattctcg aaggtctcct catcgaggtt tagttttctg ccgttgatct ctttcggtag
     2101 cgagtagtaa ttgacgttgg cgtccgatgt ccacgaccag agccagactc ccgttggagg
     2161 ggacgtctcg gccggtccgg ccttttcggc cgtgaagttg aggtttacaa cacggtcgag
     2221 cctggctctg ttgtagttgt aaaccacgac gtgaaccggt agatctccgt tgaagatctc
     2281 cgccgggata gcgaaagcgg ctttcgacat gccaaaagac gcaaaatctc taaccccggc
     2341 ccagggaacg tatccgacgc ccacgtatag aacatcccag acatcgtatt ccagatcgct
     2401 tgcatactcg atattcagat aaacgttggt agatacatct gtcggatcta tctcgttgcc
     2461 gttgccgtcc agcagcgtga tctcgatttc ggggtaagaa ccgtcgagag gatcgatcat
     2521 ggccctgttg atcgccatgt caacaggcaa ggggctgcct gcggaggcca gttcgttgga
     2581 cggaataagc ggtacagcca gtccctcgag aacgctcgga gcaagaccgg ttctttctgc
     2641 cttcacagtc acttccctga cctctagaag acccggggac aggaaccaga gaagatcaat
     2701 ctcggccttt ccttcgagat cggtctctcc ggaacccagg agcgcgcccg tttcgctgtc
     2761 gaagacctcc agggaaactc ctgcggcggc cggtcccgtc atgtactcca ccagatttat
     2821 caggagcgta ttcccgtact cctgcatgat ttccaccgtt tctgtttcag attccacgtg
     2881 tacggccggt tgtccgtagg tggcttcgta tttgcccttt atctctcctt cgaccttcac
     2941 ctggtgagtt ccggccgcga gagtcacggt tttgctggtt tcctggcttt cgtactcggc
     3001 ctctccaaca tgaagagtgt aactggccga tactatctcc gcagttgtgt tgagcttcca
     3061 gcttatagtg gcttcattgc ccgaaacgac gatcgatgtt atctcgagag tcgcttcggt
     3121 cggtttgagt ggtttgatac aagaagtaac cacaagcatg gcagcaacga tcactaccag
     3181 taacaatatg cttttcttca tccctctacc tcctttataa ggatatcaga gttttttagt
     3241 tccatttgat cgttatgatt atacatattt catttccttc aaagcgagaa cttgaaagag
     3301 tctttcggga agattctgca acgtgttttt ccacacattg aaccagtttt ttctgtcagg
     3361 tgctaaaatt ggataactta cgaaaaaggg ggtatgtgga tgaagcgtat ttcttttatg
     3421 ctggttcttc taacggcact ggtcctgctt gtaacttcct gtttcaagct gccgttcgtg
     3481 gtggtcacac tcagctccga cgcggggtcg aacacggttc ttgcgggcac caccataacg
     3541 ttcagctgga agttcgagac ggccgacgat ttcaaggagg tagtctataa gctccatatc
     3601 aacgacgata tccttgaact agagacaacc gaatatgcca ccgagctggt ggccggcaat
     3661 tacaaagttt gggtggaagc tctggggaaa ctggccatcg agagcaattt ccgcactttc
     3721 aactcgaacg agctggaatt caccgtcggt agcccttcgg ttatcttcga caacacccag
     3781 gccgcctacg cctcgaaaga tgtcacggta tcctggaaac ctgtcgacgg actgactcac
     3841 acctatctct actcggttga cggggcggcc tttgtcgaga ccgacgcctt gaaagtcgat
     3901 cttaaaagcc tggccgatgg cactcacttc gtcgaagtaa agacgaaaga gttcaacggt
     3961 ctccccagca agtacgtctt caaagtcgat actacaggac cttccatcac tatcttcggc
     4021 tcggacagat acaacggagg tctgggcgac ggagacttca gacctttcgg aaggtacatc
     4081 ttcgccagct ggcagttttc cgaaccggca gtgaaggtgg agataaggtt cagagagacg
     4141 accgaagcgg gatatccctg gatcaccgac tggctggact gggatccggc agtcagcggt
     4201 attcttctca acgacgagct ggtaacgctc tacctcacgg aaggtacgtt catgatgccc
     4261 tatatgttgg agcttggaca cacctacctt ctgtatgtca gggcttacga cgagctgggc
     4321 aacaacaaga atacctacgt gagcttcaaa cttgaagacc gctacaccgg cgaggacaaa
     4381 cccgagatct tcatagcacc gatcacggcg cagccggcga cggatgaaga ggacggctcg
     4441 ctggtaatcg cagtcgtggc acctaatgta aaggagtaca tcgacaagat caaggaatac
     4501 ttcgctccgg acaacacgcg caacggagag ctgatgtacc tccagaattc gctcctttat
     4561 tcgcagggtt taacggtcac tcaagtcttc ttccccgatt tcattcccga caagaaaaac
     4621 gtcagcaact tcgtggattt cggcgatgag ctagttcttg tgagaggctt cgtaaacgaa
     4681 ccggcggaaa tcgaagaagc gggcgatgtt gtagccttcg tagaattcgt tttcgggccg
     4741 gagatgaacg gacaggaagt ctctctggga ctcggtacgg attacttcat aagggacgga
     4801 gccaacagaa ccatcgacgg tatcgtggta gataacttta tatacggtct cgatctgccc
     4861 gaaatcgtcg gggcggaaat gtgaggggtg atttgaatga agaagacctt actggtaata
     4921 ataatcactc tggtttctat tgctactttc ggaaacgagc ttcttcagga agccggttcc
     4981 agggcggccg aagtcaacga aagtatcgca cgtttgaatc ttccctggag ggccggcgtg
     5041 cccggcgcac tcagtgaatt cgcggctcac ggcatagtcg atcttgacag cataatcgag
     5101 aagtggcacg gagaggtcga gctccccgaa aggatgaaga tggacctcac caaatcactc
     5161 ttcgtagatc aggccactac acggtacagt cagttgatct cggcgctgtt cgtttatttc
     5221 ccctatggag acgtgcacat tcccgaaggt agtttcgtgc aggttcacac tcctataacg
     5281 aaccaggcct tccacggcac ctgctgggcc ttcgccacgg ttgcctcttt cgagagcggc
     5341 ctgctggtgc agaaagacgg tcttgccgga gaagagatca ccgagccctg gaagttgcag
     5401 cacgatacct acgatctctc ggagcaattc gcctctttcc acaacataga ctgggacatc
     5461 tatttagaat cgatctacga tcctttgcag tcggatgtaa taatccagga cgacagcagc
     5521 gatgccggtg gaaacgcgat cttctcgacc tacaacagcg taaggtacgg gctcccgctg
     5581 gaaagcgatt tcccctacag catgttcgat ttcaacccct ggatactctg gaatccagtc
     5641 aacaacaact gggaagagaa catagtcaag agcaccaaga ccgtgcaggt ttactacggt
     5701 gatgaactca gctggctggg ctatccgtac gaattttaca acggcgccat caaagaggcg
     5761 atgatcaagt tcggatcgct cgccgtctct ttcagagtgc cggcagattt cgactactac
     5821 accgaaggcg tttacattcc gacctccacg actataacag gcggtcacgc cgtaactctg
     5881 gtcggctggc tcgacatggc cggactcaag gcggccttcc ccgatcttat cggtccggaa
     5941 gcgacttcga tcaccgtcaa cgatcccttc tccggtctta cctgggaagc gaccgagttc
     6001 tggataatca agaacagctg gggcgactgg ggctggaacg gttactacgt ggtaccggcc
     6061 gtctctaagc agctctacga gttctctgcc aacaacggtt ttggtatcag tccgtggatg
     6121 atcgaataca acgccatgta cgttccgata ttcgaggaaa cctacgcgat ggaagacgtc
     6181 gatttcaacg gcgacggagt ggtggacgag aaggatttcg atatcctcgt caatcacatg
     6241 tacaccgaaa gcatcgttca cgatatctca atccccaaag attactgggt cgatcacgaa
     6301 gatatcagca gattcttact catctggaac agtataatcg actaaaacag gacagcattt
     6361 caaaagcgga cctcagggtc cgcttttttt gtcgaaatcg actatcggcc gacatcaatc
     6421 aagggcaggt tttggatttc cgcgcaagat ccgctcggct ttaaagggca aactcgttac
     6481 tttccggcgc catgataaag cagcccgtta gtgtagaatt gaaacaactt aatcaaaaag
     6541 ggggaccatg aatgagacgt cttgcatctc tgctgctcgt ggtttttctc atctttctgc
     6601 tgagttcatg tttcaagcaa ttgaatgtat cggtagagtt cgaaaatctc cagaccgaat
     6661 acgcgacgaa gaacgtaacg gtttcctgga agcctaccgg tggagtcgat catctttacc
     6721 tatattcgat cgacggagac ggttatgttg aaacttccga aactgagtta gaactcacca
     6781 atcttgaaga gggacagcat acggtcgaag tgaaaatcgc cggttcgacg cgcattcccg
     6841 acaagttcac cttcatagtg gacactgaag gtcccctcgt aaggtttatg ggatcggaca
     6901 ggtatagcta cggcctgttt tccgaagact tcacgccctt cggcaggtat gttttcttga
     6961 agtgggagtt ctccgaggaa atatctaagg tcgagttcag gttcgtggac acgaattccg
     7021 aagaatttca gtatatcacc gactggctcg aatggaaccc cgccagcact tccatgctca
     7081 taagcgacga agggctgacg gcgaacctga aggaaggaaa taccgttctg gatgtcttcg
     7141 aagtcgggaa gatatacagg atgtacataa gggcctacga caaactcggg aacactcgct
     7201 acagcgatca gtacagaccc ttcgtggtct tcagactcga cgaaagatac gtcgatgaag
     7261 acaaacccct ggtctatctg gagccgggac agattttgcc ggccgaggga gagaaccgag
     7321 gttcgataga agtggccata gtggcttcca acgttgcaaa atatgtggaa agcgtaaagg
     7381 aatacttcgc acccgacaac acgcgcaacg gagagctcat gtacctccag ttctccgtag
     7441 tccacgatga aggactcgta tttgaagagg ccttcttccc gcagttcata gaaaacaaga
     7501 ccagtttcga cggggtcatt ccctcgaaca gggaagccgt cgtcgtaaga ggtttcatcg
     7561 acgaagcacc gttaatcccc gccgtatcgg acatcgtcgc gatcctgaga ttcagtttcc
     7621 ctcaagagct gagcggagag agcgtgagct tcaagatcgg ctcgcgcccc aaggacgctc
     7681 tctcgggacc tatgtatttt ataagggacg aggcgaacag ggtcatagac ggcataaccg
     7741 tggacaacca gtttgtagaa gtctcgctgc cggaactcgt ggcggccggt atgtgagggg
     7801 ataacgatga ggaaaacact aattattcta gcgctggttc tactggcctg tgcatctttc
     7861 gcgggtactc ttctcgagac cgctgccgtg agatcggccg aggtagagcg ggccataagc
     7921 gaactcggtt tgccctggag ggcaggggtt cccgctattc tcaaggagta cgccgccaac
     7981 ggagtatccg atctggacag cctgatagac aaatggaacg gaacgctcga gttgctcgag
     8041 ccgatcggag cgcctgacgc ccaggacttc gaggtgcagg ccactacacg ctacaaccag
     8101 ctgttgtcgg cgatggtcaa ttacgactac tgcggcgacc tcttcgttcc cgaagggggt
     8161 ttcattcagg tacatcccgc cgtcaggaat cagggagccc acggaacatg ctgggccttc
     8221 gcgaccgtcg cttctttcga gagcgcgctt ctggtgcagg aggatagact ccccggcggc
     8281 tggagttacg ccccatggga ataccagtac gatctctaca acctctccga acagttcctg
     8341 gccttccata acgtagatga cgatatctat ataaattcct taagagatcc tctccagtca
     8401 gatgtgataa tacaggattc taacgaagac actggaggtt ggccgatatt ctcgacctac
     8461 aactgcgtca gatacgggct ccctctggat agcgacttcc cgtatatcaa gtacgacagg
     8521 aaaaactgga tagtctggaa tccgacaaac aactactggg aggataacat aatcagaagc
     8581 accaaaacta tcagaattta cggcggcagc acgttgatga gcttccttcc gtacgatgaa
     8641 tacgtgatgg cgatcaaaga gctcctggta aactacggcg ccctcgtcac ctccttcgaa
     8701 gttcccgtag acttctctta ttacaaagag ggagtttacg taccctctac cgcgacaatc
     8761 aggggtggcc acgccgtcac gctggtcgga tggctcgaca tggatgggct caaggccgcc
     8821 ttccccgatc ttatcaaccc ccaggcaacg tcgatcacga tcgaagatcc tttcatcgag
     8881 gagcagctct ttgatctgga cgaatacaag tgggaggcca ccgagttctg ggtaatcaag
     8941 aacagctgga gcgaagactg gggctggaaa ggttactacg ttgttcctat gatttccgag
     9001 gaactgtaca acttcctcat cgaagagttt tacgcccttc caaaatggat gatcgagtgc
     9061 gacgatatgt acgtcccgct cttcgaagaa agctacgaaa cgagtgaaga tatagacttc
     9121 aacggagacg gcatcgttga tagggccgac ttcgagctcc ttctggcaaa catgttcacc
     9181 accagcgaga agtacgacat ctccattccc aaagacggcc gggtcgatca ggaagatgcc
     9241 tcgcgcttct ttgtgatctt caactccatg ctcgactgat attccacaaa aagcagggct
     9301 ttgagccctg ctttttttcg attcgatcag aaggcgctga agagggggga gtgcccatcg
     9361 gcgatgatcc cgattccttc actcatcagg actttgaaag cgatcctcct gcccggatcg
     9421 ttatcgtact ctacatagcc ggccattaga acgtcctttc cttcaaaaaa cccctccatc
     9481 ccaccgtacg gcacgaccga ataactgtaa tggccaggag aggcc
//
LOCUS       DAOOLY010000070         9499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8403_length_9499_cov_4.249576, whole genome shotgun sequence.
ACCESSION   DAOOLY010000070 DAOOLY010000000
VERSION     DAOOLY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8403_length_9499_cov_4.249576"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PLD15_05030"
     CDS             <1..1229
                     /locus_tag="PLD15_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB63300.1"
                     /translation="IPYLCLIGSEKSVAKHELKPGEYLLKGPGYLHYLPEIVFSMLEK
                     HRLQKTLDFEREKYMGLVNSMGCGMLIIRKRDGNVIFSNKVAQRLLGYEESEIEKTRL
                     QDLVYDEPLALNLVLEREGVNSDREIVMLTRTNGKIYVIFNTSEMLYGAERVVQFTFM
                     DISKQKRDREELIFQWRFLDNAEEIAIAVDERGRIVYLNRFAAEIHGYGREEMIGNNL
                     SNYLVQDDMEAKSRQFEIMSSGKWKGRQLHRKKDGSVFVVESKRSVLRVDDNVYELVI
                     GMDLTESIELQRSYNLHSLLLNGTEDIAIATDMDGRVIYMNKAAQKFFRTSLESIRHK
                     LPEEIPSESLREFLRNGLSSEPCDEGSRIITVVDDSTNSNRVEFSGKVVRDEEGDVGT
                     IFIGRRIAGSGSKSVI"
     gene            complement(1239..2954)
                     /locus_tag="PLD15_05035"
     CDS             complement(1239..2954)
                     /locus_tag="PLD15_05035"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPB63301.1"
                     /translation="MIIRTAMAQINTTVGDLEANKKIILGSIDRARSTGAGILLFPEL
                     TITGYPPEDLLLHTGFLRDNLRVLKEVAAYTTGSDTMVVVGFVDFPMEIYNAAAIIQN
                     GEIKGVYRKMNLPNYSVFDERRYFTPGSRPLIIKSGSLKIGVNICEDIWVPAGPTNDQ
                     AIAGANLILNLSASPFTSTKNRTRMSLLQTRAMEYSCCMLYCNYAGGQDELVFDGRST
                     AIMPDGHRIVARSFEEDFLVIDIDTDQSTRYNLLEGKRKGYDRKVEVEEINLKIPHRK
                     SQEFLPEETYKYSSEEDDLIRSLELGLRDYAKKNGFKKVVLGLSGGMDSSLVAVLAVR
                     ALGKENVKGVMMPSRITSDESKKDAVELAENLGVETFEIPIESVFDSINGLMRPVFSD
                     RPHDVTEENFQARIRGIILMGLSNKFGWLVLVTGNKSEMATGYATLYGDMAGGLAILK
                     DLYKTQIYRVAERINEQAGTEIIPRNVFLKAPTAELREGQRDQDSLPLYEILDGILRL
                     YIEESFSLDEIVSKGFDRKTVEYVLRLLQRSEYKRKQGAPGIKVSKRAFGKDWRMPIT
                     NRYID"
     gene            complement(2951..3868)
                     /locus_tag="PLD15_05040"
     CDS             complement(2951..3868)
                     /locus_tag="PLD15_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPB63302.1"
                     /translation="MSGLKGTFIALVLLINAAVLSVVFFTDDGTAVERIVELLESAGR
                     EVILVSYSLDDREVMESLERLGARGVDIKIMIDDSTVGRALGELTTLEMLTDSTQALI
                     HSKFILVDRERVLFGTGNFTQGSLREDSNSFIQIDSKELAGIFVDFYEAIISGESREP
                     KRFENMEFYLCPSEKAKERVISELMKARHEISFAMFAFTDPSVLSALKFKASRGVRVS
                     GVLDSWNLSSPLEDFLSTGMEVIWGDASFTIHDKTFVIDGRKVITGSANATLSGWGKN
                     REIVAIIDSEGLSRQYREHIEYLKGGGVR"
     gene            complement(3908..3983)
                     /locus_tag="PLD15_05045"
     tRNA            complement(3908..3983)
                     /locus_tag="PLD15_05045"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3948..3950),aa:Ala,seq:ggc)
     gene            complement(4040..4963)
                     /gene="secF"
                     /locus_tag="PLD15_05050"
     CDS             complement(4040..4963)
                     /gene="secF"
                     /locus_tag="PLD15_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB63303.1"
                     /translation="MTFNIDFIGKKKYYITLSLLLIAVSLVFFFTKGFNFGVDFTGGI
                     EVSVSVPDENMTVAEVRGLLSAEDANLASARIIKQRPLLEEGTEEESGRFSIIINSNE
                     NEEIIRGKMLRALGSEGVTDKNILSISTISGYAAQEIRGYAWIAVVVSMALLLLYITI
                     RFKFSFAVGAILALVHDVVIVLGFYSIFGIEINAPVIASLLTLVGYSLNDTIVVYDRI
                     RENRKKMRGADGIETIVNRSINEVIVRSFNTSFTTFIAVLTLYIFSGEVLQPFAFGML
                     VGVVVGTYSSLYIASPIVITWLKSSENRKHA"
     gene            complement(4960..6471)
                     /gene="secD"
                     /locus_tag="PLD15_05055"
     CDS             complement(4960..6471)
                     /gene="secD"
                     /locus_tag="PLD15_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPB63304.1"
                     /translation="MRANQKRLIVTVVVLVGALLPLLIPHGNAPSGSSFLDKIAANIK
                     LGLDIKGGALLEYQMVTDVSKEEMSALADRVIEVLRTRLDAAGFTEATVEKITTAISF
                     GEDIPPVRVRVQIPGITDVLRAESLVGKTGKLYFADVLAIETSSSTPSIPAGMSLEIS
                     RRKLQGGEPFWIKELHFGEYVNGVQNNNWYFISPRIAVGSSYVELDGSVVTEAKSLVN
                     NQPRPGQGRFMVSLKFNSEGSRTFREITSVKSTYTDTDIRKRLAIVLDENVIIAPLVG
                     TQISDGNAVIEGLNSLDEAKEVSILVGSGNLPVELSAFNKRVLSPTLGRDIINASLWA
                     GIGGLILIMIYMLVIYGKMGIVADIALLYNGVLLMGMVSLTGSILTLPGIAGIVLTIG
                     MTVDGNVLIFERIKEELQLGKTTENAIDSAFSKVVWTIFDANITTILAGIVLFYFGTG
                     TVKGFAITLIIGVIGAMFTNIVVSRTVLTAMAGGIKPERYLKTKKVNEGREAR"
     gene            complement(6538..6927)
                     /gene="yajC"
                     /locus_tag="PLD15_05060"
     CDS             complement(6538..6927)
                     /gene="yajC"
                     /locus_tag="PLD15_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPB63305.1"
                     /translation="MLPILSFIAYAPAGDAAAPTTDAGATSGGFTGIIIWLVLMVAFF
                     YFLIIMPQRKRDKQFKQMMEKIKVGDKVVTAGGIIGKITLIKPDSLRLRTGTGTELDV
                     TRKAIAVVVNRGEGDKEEIEPDVTVKQ"
     gene            7089..7643
                     /gene="frr"
                     /locus_tag="PLD15_05065"
     CDS             7089..7643
                     /gene="frr"
                     /locus_tag="PLD15_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPB63306.1"
                     /translation="MSSLLKDAENRMNKSVEKIVEEYGQMRTGRPSPALLESVKVDYY
                     GVPTPINQLATITATEDRTLIIKPWDKTVLSQIEKAIFASKLDLTPMNDGVNIKLNFP
                     TPTTEQRDKWVKIARENAEKGKIAVRNIRRDVIKEARDLQKASEMTEDDLKKFEEDIQ
                     ELTNKYVLEMDKAFEKKSKEIMEF"
     gene            7643..8338
                     /gene="uppS"
                     /locus_tag="PLD15_05070"
     CDS             7643..8338
                     /gene="uppS"
                     /locus_tag="PLD15_05070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPB63307.1"
                     /translation="MSLPGHVAIIMDGNGRWAEKRGLERIEGHRRGAEVADAASHWCA
                     DLGIKYVTLYAFSTENWKRPEREVDFLFELMLMYIASQLQNMLDESVRMRFIGRINDL
                     PEKLRGFTKLIEARTAGGKKLTVIVALNYGGRAEIVDSINRFIESQKTDEKRRITEED
                     ITGNLYLPEVPDPDLIIRTSGEERLSNFLMWQSIYSEFYFTKTLWPDFTKEEFLEIME
                     EYSKRKRRFGAHQ"
     gene            8338..9186
                     /locus_tag="PLD15_05075"
     CDS             8338..9186
                     /locus_tag="PLD15_05075"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPB63308.1"
                     /translation="MKKLSETTIRIVTAAVIAPFVILCFVSYFSFIGLVSSVVLFASY
                     EYLKFSLKNSNHDVVRVVISGIIPAIIVVYGILLYRSSLMSFQRPELIFAVGVISLSS
                     IVIMTVSDVKSAKDIIVNSVFSLIYVGFDLAFFYHIYLRFGSSMALLALTSVWLFDTG
                     AYFSGKRFGHIRISPSYSPKKSLEGVIGGYVTTILFMIAFIYISGLLGLYNGPELGLF
                     QIAILAMVVSIFGTIGDIVESSFKRYHGVKDSGNLLPGHGGMLDRIDGVLFVTPMFYI
                     FLTLLT"
     gene            9199..>9499
                     /locus_tag="PLD15_05080"
     CDS             9199..>9499
                     /locus_tag="PLD15_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPB63309.1"
                     /translation="MRYMTGAEIRESYLKFFESKNHVRLPSASLIPNDPQLMFTVAGM
                     VPFKPIFWGKVEPTYKRVATCQKCVRTNDIENVGRTPRHQTFFEMLGNFSFGDYFK"
BASE COUNT         2336 a         2381 c         2349 g         2433 t
ORIGIN      
        1 ctattcccta cctctgtctg atcggaagcg agaaatccgt agccaagcac gaactcaagc
       61 cgggcgagta tcttctcaag gggccggggt atcttcacta tctcccggag atagtctttt
      121 cgatgcttga gaagcacaga cttcagaaga cgctggattt cgagcgagaa aagtacatgg
      181 gtctggtaaa cagcatggga tgtggaatgc tgatcataag aaaacgcgac ggcaacgtga
      241 ttttctcaaa caaagtcgct cagagacttc tgggctatga agagagcgaa atcgagaaga
      301 cacgcctcca ggacctggtg tacgatgagc cgctcgcact gaatctcgtt cttgagagag
      361 agggtgtcaa ttcagataga gagatagtta tgctgacccg caccaacgga aagatatatg
      421 ttatcttcaa cacctccgag atgctttatg gcgccgagag ggtcgtgcag ttcactttca
      481 tggatatatc caaacagaaa cgcgacaggg aagagctgat cttccagtgg cgcttcctgg
      541 ataatgccga ggagatcgcc atcgctgtgg atgaaagggg gagaatcgtt tatcttaacc
      601 gttttgccgc cgaaattcac ggctacggca gggaagagat gatcggcaac aatctgagca
      661 actatctggt gcaggatgat atggaggcca aatccagaca gtttgaaata atgagctctg
      721 gaaaatggaa gggcagacag ttgcacagga agaaagacgg ctctgttttc gtcgtcgagt
      781 ccaagagaag cgttctccgt gtcgatgata acgtttatga actggtcata ggcatggacc
      841 tgaccgagag catcgagctt caaagaagtt acaatcttca ttcgctgctg cttaacggca
      901 ccgaggacat agcgatcgca acagatatgg acggccgtgt tatttacatg aacaaggccg
      961 ctcagaaatt cttcagaacc tctcttgaat cgattcgcca caagctgccc gaggaaatac
     1021 catcggaaag tctgcgggag ttcctcagga acggactctc ctctgaacct tgcgatgaag
     1081 gctccagaat aattacagta gtggatgatt caaccaattc aaaccgtgta gagttcagcg
     1141 gcaaagtcgt cagagatgaa gagggcgatg tcggcacgat tttcataggg cggagaatag
     1201 ccggttctgg cagtaaatcg gttatataaa acctttcttc aatctatata cctgttcgta
     1261 attggcattc tccagtcctt gccaaaggcc cttttcgata cttttatgcc cggtgccccc
     1321 tgtttgcgtt tgtattcgct tctttgcagg agcctcaaga catattcaac tgtctttcta
     1381 tcgaatccct tgctcactat ttcgtccagt gaaaagctct cttcgatata aagtctgagt
     1441 atgccgtcca ggatctcgta aagggggagg gagtcctggt ctctctggcc ttctctgagt
     1501 tcggccgttg gcgctttcaa aaagacgttg cggggtatga tctcggtgcc ggcctgttcg
     1561 ttaattctct cggccaccct gtaaatctgt gtcttgtaga ggtctttcag tatcgcgaga
     1621 cctccggcca tatcgccgta aagtgttgca tatccggttg ccatttcact tttgttgccc
     1681 gtaaccagga ccaaccaacc gaatttgtta gacagtccca tcagaatgat ccccctgatt
     1741 ctggcctgaa aattctcctc ggttacatcg tgcggtctgt ctgagaacac cggtctcatc
     1801 agcccgttga tcgaatcgaa gacgctttct attgggatct cgaatgtctc tactcccaga
     1861 ttttcggcca gttcgacggc gtcctttttc gactcgtcgg acgttattct ggagggcatc
     1921 atcacgcctt tgacgttttc cttgccgagc gctcttacgg ccagtacggc aacgagtgag
     1981 gagtccatgc caccactgag accgagaacg acctttttga acccgttctt ctttgcgtag
     2041 tccctcaggc ctaactccag tgaacggatc aggtcgtctt cctcggacga atatttataa
     2101 gtctcttctg gcagaaactc ttgagatttc ctgtggggta tttttaagtt tatctcttcg
     2161 acttcgacct tcctgtcgta gccctttctc ttgccttcga ggaggttgta gcgcgtcgat
     2221 tgatcggtgt ctatatctat gacaagaaaa tcctcctcga aggatctggc gactattcta
     2281 tggccgtcgg gcattatcgc ggtacttctg ccgtcgaaaa cgagctcgtc ctgaccgccc
     2341 gcgtaattac agtaaagcat gcagcaggag tattccatcg ctcgcgtctg gaggagcgac
     2401 attctggttc ggttcttggt cgaagtgaag ggcgaggccg agaggttcag aatgaggttc
     2461 gcgccggcta tcgcctgatc gtttgtcggt ccggcgggaa cccagatatc ctcgcatatg
     2521 ttcactccga tcttcaggct gccgctcttg attatcagag ggcgggagcc gggagtgaag
     2581 taccgtcttt cgtcgaatac ggagtagttg gggaggttca tctttctata tactcccttt
     2641 atctcgccgt tctgtattat cgccgccgcg ttgtatatct ccatcggaaa atccacgaac
     2701 cccaccacaa ccatcgtgtc gctaccggtc gtgtatgccg cgacttcctt cagaaccctg
     2761 agattgtcgc gaagaaaacc cgtgtgcaac agcaggtctt cggggggata tcccgttatc
     2821 gtgagttccg ggaagagaag tattcccgcc ccggtggatc tggccctgtc tatcgaaccg
     2881 agaatgatct ttttgttggc ttccagatcg ccgaccgttg tgtttatctg ggccattgcc
     2941 gttctgatta tcaccgtacg ccccctcctt tgagatactc tatatgttca cgatactgtc
     3001 tggaaagtcc ctcggaatct attatagcaa cgatttctct gttcttgccc cagccgctca
     3061 gggttgcgtt tgccgagccg gttatgactt ttctgccgtc tatcacaaag gtcttgtcat
     3121 ggatggtgaa agaggcgtct ccccaaataa cctccattcc ggtggagagg aaatcttcca
     3181 gaggagaaga gaggttccag ctgtcaagaa cgcccgaaac ccgaactccg cgggatgcct
     3241 tgaacttgag agcggaaaga actgacggat ccgtgaaggc gaacatggcg aacgagatct
     3301 cgtgtctggc cttcatgagc tcgcttatca ctctctcctt tgccttttct gacgggcaga
     3361 gatagaattc catgttctcg aagcgcttag gctctctcga ttccccgctt attatagctt
     3421 cgtagaaatc gacgaagatc ccggccagtt cctttgaatc aatttgaatg aaactgttgg
     3481 aatcctccct gaggctgccc tgagtgaagt tgcccgttcc gaacagaacc ctttctctat
     3541 ccaccaaaat gaacttggag tgaatcaggg cctgtgtact atcggtgagc atctcgaggg
     3601 tggtgagttc tccgagggcc cttcctacgg tcgaatcgtc gatcatgatt ttgatatcca
     3661 ctccccttgc ccccagtctt tccaggctct ccatgacctc tcgatcgtcc agtgagtaag
     3721 agacgagaat aacctccctc ccggccgact cgagaagctc tacaatcctt tcaacggcag
     3781 tcccgtcgtc tgtgaagaaa accaccgaaa ggaccgccgc gttgattaaa agcaccagtg
     3841 cgataaacgt ccctttcaag ccactcacct ctaccagaaa aaaaacaggg agccaaagct
     3901 ccctgtctgg tggagacgaa gggattcgaa ccctcggcct atacaatgcc attgtatcgc
     3961 gctcccaact gcgctacgtc cccactccag aaaagattat acactaccgt ccttgacttt
     4021 tcaacaggtt aacaatcgat caggcgtgct tcctgttttc ggaactcttc agccaggtta
     4081 ttacgatcgg actggctatg tacagggacg agtacgttcc tacaacaact ccgaccagca
     4141 ttccaaaagc gaatggctga agtacctctc ctgagaaaat gtacagtgtc aaaacggcta
     4201 tgaacgtcgt gaacgatgtg ttgaaggatc tcacgataac ttcgtttatg cttctgttga
     4261 cgatcgtttc gataccgtcg gccccgcgca tcttctttct gttctccctt atcctgtcgt
     4321 aaacgactat cgtatcgttc agagagtatc ccaccagagt cagcagcgaa gctataaccg
     4381 gagcgttgat ctcgatgccg aaaatgctgt agaaacccag aacgatcacc acgtcgtgaa
     4441 ccagagcgag tatggccccg acggcgaaag agaatttgaa ccgtatggtt atgtacagca
     4501 ggagtaaagc catggatacc accacggcta tccaggcgta gcccctgatc tcctgggccg
     4561 cgtatccgga tatggtgctt atggagagga tgttcttatc ggtgacgcct tcgctaccca
     4621 gcgccctgag catctttccc ctgattatct cttcgttctc gttgctgtta atgattatgg
     4681 agaacctgcc cgactcttcc tcggtgccct cttccagaag cggtctctgc tttattatcc
     4741 tcgcggatgc taaatttgcg tcctcggccg aaaggaggcc cctgacctct gccacggtca
     4801 tattctcatc gggaacggag acgctgactt ctataccgcc ggtaaaatcc acgccgaagt
     4861 tgaagccttt agtgaagaag aaaaccaggg agactgctat caaaaggaga gaaagagtga
     4921 tatagtactt cttcttgcct ataaagtcta tgttgaaggt catctggcct ctctcccttc
     4981 gtttactttt ttcgtcttga ggtacctttc cggtttgatg ccgccggcca ttgcagtcag
     5041 taccgttctc gagacaacta tgttggtgaa catggctccg ataacaccta ttatgagagt
     5101 tatggcaaag cccttaacgg ttccagtacc gaagtagaag agaacgatgc cggcgaggat
     5161 agtggttatg ttggcgtcga agatcgtcca gacgactttc gagaaggcgg aatctatggc
     5221 attttccgtg gtctttccca gctggagctc ttcctttatt ctctcgaaga tgagaacgtt
     5281 tccatcgacg gtcataccta tcgtgagaac gataccggca ataccgggaa gtgtcagtat
     5341 cgaaccggta agcgagacca ttcccatcag tagaacgccg ttgtaaagca gggcgatgtc
     5401 ggccacgatg cccatcttac cgtagatgac cagcatatag atcattataa ggatcagccc
     5461 gccgatacct gcccagagac tggcgttgat gatgtctcta ccgagagtcg gcgagagaac
     5521 gcgtttgttg aaggccgaaa gttcgaccgg caggtttccg cttccgacca gaatggagac
     5581 ttctttggcc tcgtcgagag aattgagccc ttctatgacg gcgtttccgt cgctgatctg
     5641 tgtcccaacc agaggggcaa ttattacatt ctcgtctaga acgatcgcca atctctttct
     5701 gatatcagtg tctgtgtagg tcgatttgac cgaagtgatc tccctgaaag tcctggaacc
     5761 ttcggagttg aacttcagcg agaccatgaa tctaccctgc cccggcctgg gctggttgtt
     5821 aaccaacgat ttggcttctg tgacaacact gccgtcaagc tcgacatagc ttgatccgac
     5881 ggcgattctg ggacttatga agtaccagtt gttgttctga accccgttta cgtattcacc
     5941 gaaatggagt tcttttatcc agaaaggctc tccgccctgg agttttcgcc tacttatttc
     6001 cagagacatt cccgcgggaa tagatggagt cgaactcgat gtctctatgg caaggacatc
     6061 ggcgaaatac agcttacctg tctttccaac aaggctctcg gcgcgcaaca catctgtgat
     6121 tcccgggatc tggaccctca ccctgacggg aggtatatcc tcgccgaaag agatcgcggt
     6181 cgtgatcttt tcgacggttg cttcggtgaa accggcggca tccagcctgg tcctcaaaac
     6241 ctcgattact ctgtcggcca gagcgctcat ttcttctttc gaaacatctg tgaccatctg
     6301 gtactcaaga agggcccctc cctttatatc gagacccagc ttgatattgg cagcgatctt
     6361 atccaggaaa ctgctgccgg acggagcatt gccgtgcggt atgagcaggg gcagcaaggc
     6421 tcccacaagt accacgacgg ttacgatcaa gcgtttttgg tttgctctca tacggatttt
     6481 gcccgaagtt ctcggacaga cacccccttc caatatctac ggataaaact gctatagtta
     6541 ctgcttgact gtcacatcgg gctcgatctc ttctttgtcg ccctcgcccc tgttaacgac
     6601 aacggctatc gcttttctgg ttacgtcgag ttccgtaccg gtgcccgtcc tcaatctgag
     6661 gctgtctggt tttatcaatg taatcttccc gattattcct cccgccgtaa ctactttgtc
     6721 gcctaccttt attttctcca tcatctgctt gaactgcttg tctctctttc tctgaggcat
     6781 gatgataaga aagtagaaaa aggcgaccat gaggaccagc cagatgatga tcccagtgaa
     6841 gcctccgctc gtcgcaccgg cgtcagtcgt gggagcggcc gcatcgccag cgggggcgta
     6901 tgctataaaa cttaggattg gtaacattta cattcctcct gtcgtggata gagcattctg
     6961 taagatttta ccagaataga ttatgcaatt caactgagaa tgcatttgtg ggttgtctgt
     7021 gcactcgtca cccaaaacga tgacatgata taatttccca tgactaacgg aagaaaggga
     7081 ggtttatgat gagtagtctt ctaaaagatg ccgaaaacag aatgaataag agtgtggaga
     7141 agattgtaga ggaatacggt caaatgagga cgggaagacc ttcgccggca cttctcgagt
     7201 cagtgaaagt cgattactac ggtgttccca cccccataaa tcagctcgct accatcaccg
     7261 caaccgaaga cagaaccctg atcataaaac cctgggacaa gacggtcctc agccagatcg
     7321 aaaaggccat attcgcctcc aagctcgacc tgactccgat gaacgacggt gtaaatatca
     7381 agctgaactt ccccactccg actaccgaac agagagataa atgggtgaag atagccagag
     7441 aaaacgccga aaagggcaag atcgccgtga gaaacatcag gagagacgtg ataaaggaag
     7501 ccagagatct ccagaaagct tccgaaatga ccgaagacga tctgaagaaa ttcgaagagg
     7561 atatccagga actgaccaat aaatatgttc tcgaaatgga caaagccttc gagaagaaga
     7621 gcaaggagat catggagttc taatgagcct tcccggtcat gttgccataa taatggacgg
     7681 taacggccgc tgggcggaaa agagaggact ggaaagaata gaaggccaca ggagaggagc
     7741 ggaagttgcc gatgcggcct cccactggtg tgccgatcta ggcatcaagt acgtgacgct
     7801 gtacgctttt tccaccgaaa actggaagag accggagaga gaagtggact ttctctttga
     7861 gcttatgttg atgtacatag cgagccagtt gcagaacatg ctggacgaga gcgtaaggat
     7921 gcgcttcata ggccggataa acgatcttcc ggagaagtta agagggttca cgaagctgat
     7981 cgaagccaga accgccggcg gaaagaagct tactgtaata gtcgccctca attacggtgg
     8041 gcgtgcggag atagtagatt cgattaacag gtttattgaa agccagaaaa ctgacgaaaa
     8101 gagaagaata acagaggaag atatcaccgg caatctctat ttaccggaag tacctgatcc
     8161 cgaccttata ataagaacct ccggcgagga acggctcagc aattttctca tgtggcagag
     8221 tatatacagc gagttttatt tcacaaagac tctctggcca gacttcacca aagaggagtt
     8281 tctcgagata atggaggaat actcgaagag aaaacgaagg tttggtgccc atcaatgatg
     8341 aagaaactgt ccgagaccac tatcagaata gtaaccgcag cagtgatagc cccatttgta
     8401 attctttgct ttgtgagtta cttttctttc ataggattgg tctcgtccgt tgtgctcttt
     8461 gccagctacg aatatttgaa gttcagcctg aagaacagca atcacgatgt cgtaagggtg
     8521 gttatatccg gtatcattcc cgccataatc gttgtgtacg gcattttgct ttatagatca
     8581 agccttatga gctttcaaag acccgagctg atattcgcgg tcggcgtgat ctccctctcc
     8641 tcgatcgtta taatgacggt gtcagacgtc aagagcgcga aagacataat cgtgaactcg
     8701 gtcttttcac tcatatacgt aggattcgat ctggccttct tctaccatat atatcttcgc
     8761 ttcggctctt cgatggcctt gctggcgttg acctccgtgt ggctctttga cacaggcgca
     8821 tacttttcgg gcaagcgatt cgggcacatc aggatctcgc ccagttacag tcccaagaag
     8881 agtctcgagg gtgtgatcgg cggatatgtg acgaccatac ttttcatgat cgctttcatc
     8941 tatatcagtg gtctgctcgg cctctacaac ggaccggaac tggggttgtt ccagatagca
     9001 attctcgcca tggtagtatc gatcttcggt acgataggcg atatagtcga gtcttcattc
     9061 aagagatatc acggcgttaa ggattcgggc aacttgttgc ccggtcacgg tggtatgctt
     9121 gatcgtatag atggcgtgct cttcgtgaca cccatgttct atatatttct gactttgttg
     9181 acctgaggag gttccgccat gagatatatg actggcgcag agataagaga gagctatctc
     9241 aagttttttg aatcgaagaa tcatgtgagg cttccgagcg cttccctgat tcccaatgat
     9301 cctcaactga tgttcacagt tgctggcatg gtgccgttca aaccgatttt ttggggaaaa
     9361 gtcgaaccga cctacaaaag agttgcaacg tgtcagaagt gtgtgcgcac caacgatatc
     9421 gagaacgtcg gcagaacgcc gagacaccag acctttttcg aaatgctcgg taacttctct
     9481 ttcggagatt atttcaaga
//
LOCUS       DAOOLY010000071         9453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8459_length_9453_cov_4.673973, whole genome shotgun sequence.
ACCESSION   DAOOLY010000071 DAOOLY010000000
VERSION     DAOOLY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8459_length_9453_cov_4.673973"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1292
                     /gene="aroF"
                     /locus_tag="PLD15_05085"
     CDS             276..1292
                     /gene="aroF"
                     /locus_tag="PLD15_05085"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPB63310.1"
                     /translation="MVVILRNGSGEEEIKKVEVLSSSLGLNCHISKGAEKIVIGIIGD
                     DRYTSVERFEALECVEQVVRVLKPFKLVSREFHGEDLNVEVGDVSIGNGKFVVMAGPC
                     AVEDRSMIEESAAFLSEMGVRIIRGGAFKPRTSPYSFQGLGRKGLELLREAADRYGMK
                     TVTEVIGETTLEAVSSLSDILQIGARNAQNFQLIQRVASCGKPVLLKRGFMNTVEELL
                     LSAEYIAYGGNMSIILCERGIRTFETSTRNTLDISAIPLIKMQSPLPIIADPSHASGR
                     RDLIIPLSRAILAVGADGIEVEVHPRPESALSDSKQSLNFREFEKLMRELKSLVSARE
                     LVLT"
     gene            1308..2570
                     /gene="aroA"
                     /locus_tag="PLD15_05090"
     CDS             1308..2570
                     /gene="aroA"
                     /locus_tag="PLD15_05090"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPB63311.1"
                     /translation="MKILPAKKIRAEIKVPPDKSISHRALILSSMAGERSVVHNLLES
                     GDTLSTFQVMKEIGARFSGDFERMEILPARWREAVRPLDCGNSGTTARMISGLLAGTT
                     GLHILFGDESLSRRPMKRVIEPLKKMGASIFARNDDSLLPLSIKGGDLRGCVHDLQVA
                     SAQVKSAILLAGMKAEGITTVIEPAPSRDHTERMLVSMGAKMKIDGRAVSIERGELNP
                     VFFQIPGDVSSASFFVVLAILHPDASITIRNVGLNERRTGLLRTLKKMGAEIEWMVEE
                     TSPEPCGTIVAKSSRLTGLEIDAGLVPSMIDEIPLLALAGVNAHGSTVIRGAGELRKK
                     ESDRIASTIENFGKLGIGIEEFEDGFSVEGPQRIVGGSVDSAGDHRIAMLFTVAGILS
                     EEGIEIAGSDSVRISYPRFFQDIAEVCR"
     gene            2567..3346
                     /locus_tag="PLD15_05095"
     CDS             2567..3346
                     /locus_tag="PLD15_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPB63312.1"
                     /translation="MRFCIIGHPVSHSLSPMIYTRFFEMKRIDATYEALDIQTGEFPS
                     VIDGLLKEYDGLNVTIPFKEAVVAHVDSMIERSLRAVNCIFEGAGHNTDWIGFSHSLR
                     NIEIEEPVTILGAGGASRAVVYALKRSGVHRVNILNRSPARAERVAEDFNDGNFEVKT
                     FSIDSLRPIVRESKSIVNATSIGMKGERLDLRREDLQGISLVYDLIYSETPLQKVARE
                     CGVENVIDGKAMLFFQACENLKIWRLFDEKVFERAFEVVIG"
     gene            3343..4467
                     /gene="aroC"
                     /locus_tag="PLD15_05100"
     CDS             3343..4467
                     /gene="aroC"
                     /locus_tag="PLD15_05100"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPB63313.1"
                     /translation="MRFSVGGDSHGPAMYGLVEGLPAGFPVSIDEIDKNLQRRQRGYG
                     RGKRMGLEHDRAIVKSGLWKGLTTGAPVLIEIANLAPRSEMEMRSLPRPGHADYAAWT
                     KYRLDDLTVYAERSSARWTAALVSIGSIASQMLLKLGIEIVGSVVAIGKVHCSIPESV
                     CVVARDASPVYCHDPLASCEMVAEIEKAIDRGDTLGGIFAVSAFGLPPGIGGYGELFE
                     RLDSKIGRYFMAIPSVKGVFVGNSDVSLPGSQYHDPFTVSDGRVARRSNNAGGVEGGI
                     SNGQPVVVVASVKPIPTLGNGIDSVNLKNMNVEKTPYVRSDVTAVPAASVVGESMLGL
                     LLLESILEKYGNDNFDLLKGRLENESLPCWNDGLGEKHHR"
     gene            4415..5902
                     /locus_tag="PLD15_05105"
     CDS             4415..5902
                     /locus_tag="PLD15_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPB63314.1"
                     /translation="MRVFLVGMMGSGKSTIGKILSSVLDLEFVDMDSEIEARSGKTVE
                     KIFESEGEEVFRKLESNLLKELVRKDGIVVSTGGGIVLDSGNRRTLKEENTLYLKLPP
                     ADLFKRVDTLNRPLLKGGKEAIFRIWEERRELYEQFPSIEASNYSKWETVASICMRLF
                     PGDKKRIINGTHPVTLANGGFKAIGGMQNAVVSHRVLKVFSEWIPSSAFPIEDGEEAK
                     GFHTLFEIYDFLMERGLSRSDVLAAAGGGTVTDLVGFAASTFKRGVPLTLFPTTLLSQ
                     VDASIGGKNGLNFRGGKNMIGNFYFPTETVLDPVTTLSMDPGRFEEGLVEAFKIFLIT
                     GQWYDQFRSLCGKLKGRNLATLNKMMEEAVILKDRIVASDVHEKGVRKILNLGHTLGH
                     LYEPIVGVSHGRAVAWGLEREMYYFMNLGLVEESVYNEVCDTLSEIFGLDLPPLPIEE
                     ALRLLRNDKKGTACDEAEIEIPLLRSPGKYELLKVKFDELLAVVV"
     gene            5899..6321
                     /gene="aroQ"
                     /locus_tag="PLD15_05110"
     CDS             5899..6321
                     /gene="aroQ"
                     /locus_tag="PLD15_05110"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPB63315.1"
                     /translation="MKILVMNGPNLNMLGKRKSDIYGGLSYEDLIDIIKARALDLEVE
                     CEFFQSNHEGELIDRIHGLDFDALVINPGALTHYSYALRDALEIFTGPKIEVHISNIH
                     AREAFRNRSVVSPVCTGTIAGLGFTGYLLAIEFIAGQR"
     gene            6373..6849
                     /locus_tag="PLD15_05115"
     CDS             6373..6849
                     /locus_tag="PLD15_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63316.1"
                     /translation="MKKTLLFVLPLLVFAISGCLNVAGRPESILKSLEKEVLRGNVDR
                     ILELYAETVELSTTANPAGRSVTKIDLRYYYDQFLAAREISSYSLDVESVEKPSSGQA
                     VIYASFTLTFRCDDRDYSVSAQTSFSLSKVNRKWRITGEWNNPILSIYEPASQLPE"
     gene            complement(6862..7236)
                     /locus_tag="PLD15_05120"
     CDS             complement(6862..7236)
                     /locus_tag="PLD15_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPB63317.1"
                     /translation="MRYWINVSIFIAVSVTLMALVSDWHRNRPVEVPKPVEARPFGEL
                     QDGIYTGTEGYAVVQVEIKNGTILSAKVLQNKPGSYSKSAETVVDRVIEAQSLEVDMI
                     TGATATSRVIVDAIRNAFDTVE"
     gene            complement(7450..8688)
                     /locus_tag="PLD15_05125"
     CDS             complement(7450..8688)
                     /locus_tag="PLD15_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPB63318.1"
                     /translation="MIVLNEEALKLHKELRGKLEVKSRVRVDSMKAMSLAYTPGVADV
                     CRVINENNELVYDYTNKWNYVAVVSDGTAVLGLGDIGPEAGMPVMEGKALLFKEFGNV
                     DAFPLCINVHEPDEIIAFVQALSPTFGGVNLEDISSPKCFYIEQELQRRLDIPIFHDD
                     QHGAAIVATAALRNALKIVGKKMENLKVATVGIGAAGGATIRMLLAAGVRNIVAVDRN
                     GILNKNDPKTLLNEFHREIVEEINPDNLSGDLAKAVKGADVFIGTSVGGIISAEMIKS
                     MAPGAIVFALANPVPEIMPDLAKEAGAKIVATGRSDFPNQINNVLAFPGIFRGALDVR
                     SREINESMKLAATDALADAVGDDRLSVDYILPKAFEPGIARRVALAVAKASLESGSAK
                     LKISPDDVEAIVDTGFKKVG"
     gene            complement(8685..9185)
                     /locus_tag="PLD15_05130"
     CDS             complement(8685..9185)
                     /locus_tag="PLD15_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPB63319.1"
                     /translation="MKIEELRAGDELLYSGELLLMRDAAQKRLKMLLEEGEELPVSLD
                     GAIIFYAGPAKPTKDSFGAIGPTTSNRMDAFLEMLYLKGVLATVGKGKRGQQAVSLCK
                     EYGRAYFLAPSGAAAALAGRISSMTVVAFDDLGTEAIFRVRVKDFPLYVAIDYLGNDV
                     FIKNRG"
     gene            complement(9175..>9453)
                     /locus_tag="PLD15_05135"
     CDS             complement(9175..>9453)
                     /locus_tag="PLD15_05135"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPB63320.1"
                     /translation="GIGGSSDKAMVLAKLALTRSFDERHHDKDYAALEERILGEINDL
                     KIGYQGLGTGITAYSVHIETYPSHIAIMPVGLATDCYIARKGRVILED"
BASE COUNT         2383 a         2242 c         2567 g         2261 t
ORIGIN      
        1 acaaaataca atcgtttcgt agagataaca atccatcggc cggaaaattt gactcgtgcc
       61 gtaggaaaag tgtagaatct acttaattca atgaggagtg cgtatatgtc tatatttgat
      121 ttttcctgga taaatgcaaa cagctattat tatattatga gcatctgctc gtgagccagc
      181 tctccgggta gatgctttcg catttactcc ggataaagat tgagagggtc tccaatccgg
      241 agaccctctt tcatttttag aatgcggggt gaagtatggt agtgatttta aggaacggga
      301 gtggtgagga agagatcaaa aaggttgagg tgttgtcctc tagtctgggt ctcaattgcc
      361 acatctcaaa aggagcggag aagatagtga taggtatcat cggggacgac cggtacacct
      421 cggttgaacg ttttgaagcg ctagaatgcg tggagcaggt ggtgcgggta ctgaagcctt
      481 tcaagttagt atccagggag ttccatggcg aggatctcaa tgtcgaggtc ggagatgtat
      541 cgataggaaa tgggaagttc gttgttatgg ccggtccttg cgcggttgag gatcggtcga
      601 tgatcgaaga gagcgcggcc ttcctctccg aaatgggtgt gagaattatc aggggaggag
      661 ccttcaagcc aaggacatct ccctattctt ttcagggact tggcagaaag ggactcgagc
      721 ttcttagaga ggcggccgat agatatggaa tgaagacggt aactgaggtg ataggagaaa
      781 caacgctgga agcggtttca tcgctgagcg atatccttca gataggtgcg cgtaacgccc
      841 agaacttcca gcttattcag agggtagcca gttgcggaaa accggtgctt ttgaagaggg
      901 ggttcatgaa caccgtagaa gagcttcttc tttcggccga atacattgcc tacggcggca
      961 acatgtcgat aatcctatgc gagaggggta taagaacctt cgaaacatct acaaggaaca
     1021 ctctggatat ctcggcgatt ccacttataa aaatgcaatc gccactgcca ataattgccg
     1081 acccgagcca tgccagcggg cgaagagatc tgatcattcc cctgtctagg gccattctgg
     1141 ccgtcggagc cgacggtata gaggttgaag ttcatcccag accggagagc gctctgtcag
     1201 acagcaaaca gagtctgaat ttcagagaat tcgaaaagct gatgcgcgaa ttgaagagcc
     1261 tcgtgagtgc cagagagctc gtactgacct gatggtggag gaaagagatg aagatactac
     1321 cggcgaaaaa aataagggca gaaataaagg ttccacccga caaatcgata tcccacaggg
     1381 ctctgattct ctcatcaatg gcaggagaac gaagcgtcgt ccataacctt ctggagagcg
     1441 gggatacctt gagcactttt caagttatga aagagattgg agcgaggttc tccggtgatt
     1501 tcgagcgcat ggagatactc ccggcgcgtt ggagagaggc cgttcgacct cttgattgcg
     1561 gtaattccgg aacgacagcg agaatgattt ccgggcttct cgccggaact acgggccttc
     1621 atatcctctt tggcgacgaa tctctatcca gacggcctat gaaaagagtg atagagcctc
     1681 ttaaaaagat gggtgcctca attttcgcaa ggaacgacga cagcctgtta ccactgtcga
     1741 taaaaggcgg ggatcttcgc ggttgcgtcc acgatctcca ggtggccagc gcccaggtga
     1801 agagcgcgat actgctggct ggcatgaagg ctgaaggcat aaccacggtg atcgaacccg
     1861 ctccaagcag agaccataca gagagaatgc ttgtctcgat gggagcaaag atgaagatcg
     1921 acggaagagc cgtttccata gaacgtggcg aattgaatcc cgtgtttttc cagataccgg
     1981 gagacgtctc atcggcctct ttcttcgtgg tactcgcaat tctccatccc gacgcctcaa
     2041 ttaccatcag aaacgtggga ctcaacgaaa gacgcacagg cttgttgaga acgttgaaga
     2101 agatgggcgc cgagatcgaa tggatggtcg aggaaacctc gcccgaaccc tgcggtacaa
     2161 tagtggctaa atcttccaga ttgacggggt tggagatcga tgccgggctg gttccttcga
     2221 tgatagacga gattcccctt ctggcgctcg ccggggtaaa cgcacacgga tcgacggtta
     2281 tcaggggagc gggagaactc aggaaaaaag agtcggacag aattgcatcg accatcgaga
     2341 actttggaaa gctcgggatc ggaattgagg agttcgaaga cggcttttca gttgaagggc
     2401 ctcagagaat tgtcggagga agcgttgatt cggccggtga ccacaggata gccatgctct
     2461 tcacagttgc aggtatcctc agcgaggaag ggatcgaaat cgccggctcc gattcggtaa
     2521 ggatctccta tccgcgattc ttccaggaca tcgcggaggt gtgtcgatga ggttctgcat
     2581 aataggccat cctgtctcac acagcctctc gccaatgatc tacacccgtt tcttcgaaat
     2641 gaagagaatt gatgctactt acgaagccct ggatatacag actggagagt tcccatcggt
     2701 tattgacggt ttgctcaagg aatacgacgg tctcaacgtg actataccct tcaaggaagc
     2761 ggtcgttgct catgtcgata gtatgataga gagatctttg cgtgccgtga actgcatttt
     2821 cgagggagcg ggccacaaca ccgactggat aggcttcagt cattcactca ggaatataga
     2881 aatagaagaa cccgtaacga ttctaggcgc cggaggagct tccagagccg tggtttacgc
     2941 cctcaagaga tctggagtcc accgtgtaaa catactcaac aggagtccgg ccagggcgga
     3001 aagggtcgcg gaggatttca acgacggcaa tttcgaggtc aaaacctttt ctatagattc
     3061 tcttcggcct atagtgagag agagcaagag catcgtaaac gcgacgtcga tcggaatgaa
     3121 gggagaacgt cttgacctta gaagagagga tttacaggga atttctctgg tttacgatct
     3181 gatctacagc gaaacgcctc tccagaaggt cgccagagag tgcggagtcg agaacgtaat
     3241 tgacggaaag gctatgctct tcttccaggc ctgcgaaaat ttgaagatct ggagactctt
     3301 cgatgaaaag gttttcgaaa gagccttcga ggtggtgatc ggatgaggtt cagcgtaggt
     3361 ggagattcgc acggtccggc gatgtacgga ctcgttgaag ggttgccagc cggtttcccg
     3421 gtatcgatcg atgagataga caagaacctc cagaggagac agcgcggcta cggaagggga
     3481 aagagaatgg ggctggaaca cgacagggcc atcgtgaagt ccggactctg gaagggcctg
     3541 acgaccgggg caccggtttt gatcgagata gcgaacctcg ctcccagaag cgagatggag
     3601 atgagaagtc ttccccggcc cggccacgcc gattatgccg catggactaa atacaggctc
     3661 gacgatttga cggtttatgc cgaacgcagc agcgcaaggt ggacggctgc gctggtctct
     3721 atcggatcga tcgcatccca gatgctgttg aaactcggaa tcgagatcgt cggttcggtc
     3781 gtcgcaatag gaaaggttca ttgctcgata ccggaaagtg tttgcgttgt ggcgcgtgat
     3841 gcttcaccgg tctactgcca cgatccgctg gcgagttgtg agatggtggc cgaaatagaa
     3901 aaggcgatcg atagaggtga cactctggga ggaatcttcg ctgtcagcgc gttcggtctt
     3961 ccacctggaa taggtggata cggcgaactt ttcgagaggc tggattcgaa gatcggacgg
     4021 tacttcatgg ccatcccctc ggtgaaggga gtgtttgtgg gcaattccga tgtcagtctt
     4081 ccaggaagcc agtaccacga tcctttcacc gtcagcgatg ggagagtcgc gaggcgttcg
     4141 aacaatgccg gcggggtaga aggggggata tccaacggcc agccagtggt cgtagttgca
     4201 agcgtgaagc cgattcctac gcttggaaac ggtatagact cggtcaatct gaagaacatg
     4261 aacgttgaga aaactccgta cgtccgttcc gacgtgaccg cagtccccgc cgcttcggtc
     4321 gtcggagaga gtatgctcgg ccttttgctt ctcgagtcga tcctcgagaa atacggaaac
     4381 gataacttcg atttattgaa agggaggttg gagaatgaga gtcttccttg ttggaatgat
     4441 gggctcgggg aaaagcacca tcggtaagat cctctcttcg gttctcgatc tcgaatttgt
     4501 ggatatggac agcgaaatag aagccaggag cggaaagact gtcgaaaaga tcttcgagag
     4561 cgagggagag gaagttttca ggaaactaga aagtaacctt ctcaaagagc tggtaagaaa
     4621 agacggcata gtggtctcca caggcggcgg tatcgtactc gattcgggga atcgaagaac
     4681 gctcaaagaa gagaatacct tatacctgaa actaccaccg gccgatctct tcaaacgtgt
     4741 tgacacactt aacaggcctc tcttgaaggg aggaaaggaa gcgattttca ggatatggga
     4801 agagaggaga gaactgtacg agcaatttcc ctcgatagaa gcttcgaatt actccaagtg
     4861 ggagacggtt gcatcgatct gcatgaggct atttccgggg gataagaaga gaataatcaa
     4921 cggaacccat ccggtgaccc ttgccaacgg ggggttcaaa gcaatcggag gaatgcagaa
     4981 cgcagttgtc tcacacaggg tactcaaggt cttttccgag tggattccct catcggcctt
     5041 tccaatagaa gacggagaag aggcgaaggg ttttcacacg cttttcgaga tctacgactt
     5101 tcttatggaa cggggactct cgagaagcga cgttcttgcg gccgccgggg gaggtactgt
     5161 gactgatctg gttggattcg ccgcttccac cttcaagagg ggagttcccc tgactctctt
     5221 tcccacgact cttctctctc aggttgatgc ttccataggc ggaaagaacg gattgaactt
     5281 tcggggtgga aagaatatga taggcaactt ctattttcca accgaaacgg ttctcgatcc
     5341 ggtgacaacg ctttcgatgg atccgggaag gtttgaggag ggtctagtgg aggccttcaa
     5401 gatcttcctg ataaccggac agtggtacga tcaattcaga tctctatgcg ggaagttgaa
     5461 gggcagaaat ctcgctaccc tgaacaagat gatggaagag gccgtaattc tgaaggacag
     5521 gatagtggct tcagatgtcc acgaaaaggg agtcagaaaa atactcaacc tgggacacac
     5581 tctcggccat ctttacgagc cgatagtcgg tgtgtctcac ggaagggccg ttgcctgggg
     5641 actggaacga gagatgtatt atttcatgaa cctcggactc gtcgaggaat ctgtgtacaa
     5701 cgaagtatgc gacacgcttt cggaaatatt cggtctcgat ctgccacctc ttcccattga
     5761 ggaagcctta cgcctgttga gaaacgacaa gaaggggacg gcctgcgacg aggccgaaat
     5821 agagataccg ctcctgagat cgcccgggaa gtatgaactt ttgaaggtta agttcgacga
     5881 acttctggcg gtggtggtat gaagatactt gtcatgaacg gtccgaacct caacatgctc
     5941 ggaaaaagga agagcgacat ctacggtggt ctctcttatg aagatttgat agacattatc
     6001 aaggccagag ccctagatct agaagtggaa tgtgagttct ttcaatctaa ccacgaaggc
     6061 gaattgatag acaggatcca tggactggat ttcgatgcac tcgtcataaa tccgggagcg
     6121 ctgacacatt acagctacgc attgagagac gcgctggaga tattcaccgg tccgaagata
     6181 gaagtacaca tctcaaacat acacgccaga gaggccttca ggaacagaag cgttgtatcg
     6241 cctgtttgta ccggtacgat cgccgggctg ggctttacag ggtatcttct agccatagag
     6301 tttatcgccg gccaaagatg agggccaaat ctggtattat tgattagatt cgtaagtctc
     6361 gggggtgtgg tcttgaaaaa gactctcttg tttgtgttgc cgttgctggt atttgctatt
     6421 tccggatgtc ttaatgtggc gggtagaccg gaatcgattt tgaaatcact cgaaaaagaa
     6481 gtgctgaggg gaaatgtcga cagaattctg gagctttacg ctgaaaccgt ggagctctcg
     6541 acgacggcca atcctgccgg aaggtccgtt acaaagattg atctcaggta ctattacgac
     6601 caattcctgg cagcacgaga gatctcgagc tactctctgg acgtcgaaag cgttgaaaag
     6661 ccttcatcgg gtcaggccgt tatttatgct agcttcacgc tcactttccg gtgcgacgat
     6721 agagattact ctgtgtcagc ccaaacgtct ttctctctct cgaaagtgaa ccgaaaatgg
     6781 agaataaccg gagagtggaa caatccgata ctcagtattt atgaaccggc atctcaattg
     6841 cctgaatgat gtgggtggcg gtcactcgac agtatcaaac gcgtttctta tggcatcgac
     6901 aattaccctg ctcgtagccg tggcgccggt gatcatatcc acctcgaggc tttgagcctc
     6961 tataactctg tcgacaacgg tttcagcact tttagaatag cttcctggtt tattctggag
     7021 aaccttcgcg gagagtatcg taccgttttt gatctcgacc tgcacaactg cgtatccttc
     7081 tgttcctgtg taaatgccgt cttgcaactc cccaaagggt cttgcctcga ccggtttggg
     7141 gacttcaaca ggtctattcc tgtgccagtc gctcaccagt gccatcaaag tcacggaaac
     7201 ggctataaaa atcgaaacgt tgatccagta tctcatcgca tctcctccag gctggcaaat
     7261 tgcgaatctt ctcaattgaa ttatcactta tttagaatca cgacaaatta ctcgtatgaa
     7321 agattatgat taaatttatc ggaattattt acgacccgct ggtgttctat actttatata
     7381 gagccagcca gaatctgtgg tctcaaaaaa gaggccgcca tgcgagcagc ctcttttttt
     7441 atatcatatt caacccactt ttttgaatcc tgtgtctact atcgcttcga catcatcggg
     7501 cgagatcttc aactttgccg agccagactc cagggaggct tttgcaacgg cgagcgctac
     7561 tctgcgcgct attcccggct cgaaagcctt cgggagtatg tagtcgacag acagcctgtc
     7621 gtcgcctacg gcatcggcga gggcgtcggt cgccgcgagc ttcatggatt cgttgatttc
     7681 tcttgatctc acatcgagcg cacccctgaa tataccgggg aaggccagga cgttgttgat
     7741 ctggttggga aaatccgatc tgccagtggc aacgatcttt gcccctgcct ctttggccag
     7801 atcgggcatg atctcaggta cgggattggc cagggcgaaa acgatcgctc cgggcgccat
     7861 ggatttaatc atttcggcag agattatccc accgaccgag gtgccgatga aaacatcggc
     7921 gccctttacg gccttcgcaa gatcaccgga aaggttgtcg ggattgatct cttcgactat
     7981 ctcgcggtga aactcgttta gcagggtttt tgggtcgttt ttattgagaa taccgttcct
     8041 atcgaccgcg acaatgtttc taacgccggc cgcaagcaac attctgatcg tcgctccacc
     8101 ggccgccccg atgccgaccg tcgcgacctt cagattttcc atctttttac ccactatttt
     8161 caaagcgttc ctcaacgcgg cagttgccac gatagccgct ccatgttgat cgtcgtggaa
     8221 gatcggtata tcgagtcttc gctgaagctc ttgttcgata taaaaacact tcggagagga
     8281 tatgtcttcc agattcaccc caccgaacgt tggagagagg gcctgaacga atgcgattat
     8341 ttcatcgggc tcatgaacgt ttatgcagag agggaaggca tcaacattac cgaactcctt
     8401 gaagagcaag gctttgcctt ccataacggg cattccagct tcgggaccga tatctccaag
     8461 gccgagaact gccgttccat ccgatacgac cgcaacgtag ttccatttgt tggtgtagtc
     8521 atagaccagc tcattgtttt cgttgataac acggcaaacg tcggcgacgc cgggagtgta
     8581 agccagcgac atggccttca tgctatcaac cctgactctg cttttcacct cgagcttgcc
     8641 acgtaattct ttgtgtagtt tcagtgcctc ttcgttcaaa acaatcaccc tctattctta
     8701 atgaaaacat cgtttccaag gtaatcgata gcgacgtaga gcggaaagtc cttcaccctg
     8761 accctgaata tagcttccgt accgagatcg tcgaaagcca caaccgtcat gctggagatt
     8821 ctcccggcca gagcggcggc ggcgccgctg ggagccagga agtaagctct tccgtactct
     8881 ttgcagagcg aaacggcctg ctggcccctc tttccttttc ccaccgttgc cagaacgcct
     8941 ttcaaataaa gcatttcgag aaatgcatcc atcctgttcg aagtcgtggg tccaatagcc
     9001 ccgaaagaat ccttagtcgg tttggccggc cccgcgtaga agattatagc cccgtcaaga
     9061 gaaaccggta gttcttcgcc ttcctcaagc agcatcttca gacgtttctg agcggcgtct
     9121 ctcattagaa gtaattcgcc ggaataaagg agttcgtcgc cggcacgcaa ttcttcaatc
     9181 ttcaaggatt actctcccct ttcttgcgat atagcagtcc gttgcgagtc ccaccggcat
     9241 tatggcgatg tgcgagggat aagtctctat gtgcaccgag taagcggtta tacccgttcc
     9301 cagaccctga tagccaatct tcagatcgtt gatttctccc agtattcttt cttccagggc
     9361 cgcgtaatcc ttatcatgat gtctctcgtc gaaggatctg gtgagagcga gtttggcaag
     9421 taccatcgcc ttgtccgaac tcccgccgat ccc
//
LOCUS       DAOOLY010000072         9353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8587_length_9353_cov_4.818886, whole genome shotgun sequence.
ACCESSION   DAOOLY010000072 DAOOLY010000000
VERSION     DAOOLY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8587_length_9353_cov_4.818886"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLD15_05140"
     CDS             complement(<1..475)
                     /locus_tag="PLD15_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63321.1"
                     /translation="MSLIKPKRRRGPRMLWLILVPVLIVAALGLYVYLTMNGVNNSNE
                     VKARYVDYLFYLEEPGGGSYYYLLTSNDGRSSLVTFPVYSTIENSKEVLDPQLEASAI
                     KLIQSWLNTSGDYSYFVRLSDTLLANLSESLGIKASNAVQLIDGMAVRGFKLLDYW"
     gene            complement(462..1088)
                     /gene="yqeK"
                     /locus_tag="PLD15_05145"
     CDS             complement(462..1088)
                     /gene="yqeK"
                     /locus_tag="PLD15_05145"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPB63322.1"
                     /translation="MDAGFFRKAVRIMGSNSHVAKDLVKKARALCTVSRFEHIMGVER
                     LAVELSRLHGEDEEKASLAALGHDIFRDLPGGELLRLANVYGLEPCILEIKQPVLLHG
                     KVAASHIRNEYGIDGDIFESIYWHVSGYHSLPRLAKILMIADMGEETRQFTQAARVRE
                     QAKSDLEKSFLNVIRLKIQWSLNSEKYLLPETVLAWNYCIGGADYVSD"
     gene            complement(1122..1466)
                     /locus_tag="PLD15_05150"
     CDS             complement(1122..1466)
                     /locus_tag="PLD15_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPB63323.1"
                     /translation="MNVALALSVIKAAISFFSGGRVMHFGYMTYLVKLEGINSLKEKR
                     GVIRPVFNDLKKNFNASIVETGRHDSREEFEVTIGIVANTRGELDSLFNSVYERILWN
                     GFEVIGESGESW"
     gene            complement(1512..3584)
                     /gene="fusA"
                     /locus_tag="PLD15_05155"
     CDS             complement(1512..3584)
                     /gene="fusA"
                     /locus_tag="PLD15_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB63324.1"
                     /translation="MNKIPVDAKRDIALIGHHGSGKTQLVDAMLFNAKLIDRTGILAT
                     DTEEVEKEKKASFSMGVTSFAHGDNRIYVLDTPGMADFMAETVNGIYACENVVAVINS
                     TAGMEIQTERFGAIAGEQGKGIIAFFNMLDKERSSYQDALADLADTFERTPVLVQLQI
                     GSEGNFKGLVDLIAMKAYIYEGNSGTFSESAIPADLAQAAEEARTKMIEDIVQNDEEL
                     MMKYLEGEELSGDEIVGALKKAYLANEVVPVLLGSALKNIGVHQLIDFVVSVGKKTSE
                     SSPRSATLLSDDKIEVNPTEEEPLVAYIFKSVVDPFVGKLTYMKLLSGSLKQGDTFVV
                     VDQDSQEKAGHVILPEGLKEIEVDEATVGDVVKLSKLKKSAVGNTATHKDRQMKLDLP
                     VLPEPMISKSIQPKSKGDIDKISGGLARLAESDPTFKWENDPETNETVISGLGSVHLD
                     IMIERLKKLFSVDVEVGKPKIAYRETVRKTVETEYKHKKQTGGHGQYGHVQIRIEPNE
                     RGGGFEFIDKIVGGVVPKNYIPAVEKGVVEAMKKGIVAFYPVVDTKVTLFYGSYHDVD
                     SSDMSFQIAARQAFKNGMTDANPVILEPIMDIEVFVPDEATGDIMGEITSRRGRPMGM
                     EPQGKGTSKVVAQAPLAEMLDFANKLSSITSGRGYFTMKFNGYQETPPDVQQKIVVER
                     QRELEEQK"
     gene            complement(3608..5281)
                     /locus_tag="PLD15_05160"
     CDS             complement(3608..5281)
                     /locus_tag="PLD15_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPB63325.1"
                     /translation="MRRIFLLLFIVACLTALSKNLVYLDERGQMMTYDDVVISTPSGE
                     FVSIEIIDKILKGVVISQSLRQEETIMILPSGAIVSFSHKTSRATVEFGNEFDNALLI
                     RDGVAYINSELLSVITRASIFSETDSLVLYSQPLRIKKIDNQADSIRITLDRKVLPDF
                     ITYWWTGSGSLAITLRPAMVDPLLDYDEVSISSGRDFVRLVFEKPWGDIEYEIDGLSV
                     IFRNKSGLGRTVQIETASSYSLSLYESYVDGRRFSMSYLEMNPEKFVFSVELASNGIA
                     GLEKASDILNRRKSEIMINGGYFDQNQNLPIGLLIRNGEVLGLPSLGRPALYVTDKGR
                     VYVSRMDIIYLAEIGGRTVQITGVNSPYRGEVVLYTDEYRNTLPEFDDFQYLVIENSG
                     RVISKGYVSFARQGTNVLVLSPSAVQKSGNVSVGSTVKFKLLNSYGENLVSAVEGGPM
                     IIHDGAPVSDYERNYYSSSLIDVRAPRTLVGVKETGEIVFIVIDGYQQSSYGLSMKEM
                     IEFFRDKGFDSLMCLDGGKSSIMMVEGKVVNTPSSGIPSLPVVIMGTRK"
     gene            complement(5289..6590)
                     /locus_tag="PLD15_05165"
     CDS             complement(5289..6590)
                     /locus_tag="PLD15_05165"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPB63326.1"
                     /translation="MRIYDIILKKRNGLENTRSEIEELVMGYVKGSVPDYQMTAWLMA
                     VYFNHLSPGERSYLTHIMLNSGERIDLSSIEGIKVDKHSTGGVGDKVTLVVGPVVASC
                     GLVFAKLSGRGLGHTGGTIDKLESIPGFTTSLDADRFKRQASRIGIAIAGQTAEVAPA
                     DKKIYALRDVTATVEEISLIASSIMSKKLAIDSDGILLDVKTGRGAFMKNFEDARELA
                     LAMIDIGERGGKKVGAVISDMDQPLGRMVGNSLEVIEAIETLKGNGPADFELLCQVIS
                     ERMLIIGGRADEQSAPGIVKDAIDSGKALAKFDGFVAAQGGPTDFSTNYRKYLSPAGI
                     IEEVKAERDGFVSGIDAEGVGLVCMRLGAGRKTKEDVIDPVVGVELVKKIGDRVARGE
                     TLAIIHGNSRELVELEKKALTETFSFSSGEVVPPPTVHEVL"
     gene            complement(6587..7720)
                     /locus_tag="PLD15_05170"
     CDS             complement(6587..7720)
                     /locus_tag="PLD15_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB63327.1"
                     /translation="MVDVPQSAEFILIYKRVQQLLAQSREAYNRLSARQENLMTILDN
                     LGEALLITSDDGRIEYVNKSARDLSSKELLGRKISEGIDNYYIGDLLDEVIRTGENQE
                     SEINMYYPKRAIKECKIIRVALKEQVKHLILLRDITKEKELEAMRRDFVANVSHELRT
                     PLTSIHGYSETLAEDDLEDRETVYRFLKIIENESARMTRLINDLLDLEKLESGDAAFN
                     FEEVELVEVANYVLRIVKPLADEREVQLNTDLEEGIFIDGDFDRLVQLLLNLVDNAVK
                     YTAAKEHGSKEVWLRIYAQNNFALIEVEDSGVGIPEESLKHIFERFYRVDKARSRKMG
                     GTGLGLAITRFIVEKHRGSIHLESEYGTGTIFKVKLPLKKVFE"
     gene            complement(7839..8558)
                     /locus_tag="PLD15_05175"
     CDS             complement(7839..8558)
                     /locus_tag="PLD15_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB63328.1"
                     /translation="MAKKSILVIDDEPSIVELLTFNLKKEGYDVLKAYDAEEALKIAE
                     DNETDMFIVDIMLPGMDGFELVRNLRATEKFRQTPVIFLSAKSEEFDKVLGLELGADD
                     YITKPFSVREVLARIRAVFRRIQQSAQAKEERPKKIIARELEIDTEKYEVRVRNRMVS
                     LTPLEFELLRFLAENEGKVFSRDVLLDKLWGYDYYGDTRTVDVHIRRLRTKIEEDASN
                     PKYIITVRGKGYKFRDPGKED"
     gene            complement(8530..>9353)
                     /locus_tag="PLD15_05180"
     CDS             complement(8530..>9353)
                     /locus_tag="PLD15_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63329.1"
                     /translation="CARKCRFCVSGYRFGKARFRDLEELLAIMERQARITKRFGLVAA
                     TVTDYPFIDRLLERSLELGYDLSVSSLRLDALSENLLEVLKKGKQKVFTVAPEGGSQR
                     IRNLFGKGINDRDIESALEMGIKKGFKNIKLYFIYGASFENESDRSGIVEIAKLAVGM
                     GYSQVVLSLNPLVPKPGTPFEELPMENLQSLKKIERELRSKLRMPGVKSDFESLKESS
                     LQYALGNIDEAGSENFLEYLKEERNAIPFLQRYVEELNLRRKEWKMHGKEEYSCY"
BASE COUNT         2028 a         2526 c         2167 g         2632 t
ORIGIN      
        1 tccagtaatc gagcagcttg aaacccctca cggccattcc gtcgatcaac tgaacggcgt
       61 tggaagcttt gatccctaga gattcggaaa ggttggccag gagcgtgtca gagaggcgca
      121 cgaaatatga ataatcgcca ctcgtgttta gccagctctg gatgagcttt atggctgagg
      181 cttccagctg cggatcgagg acctctttgg agttttcgat cgtcgaatag acagggaagg
      241 tgaccagcga gctcctgccg tcgtttgagg tcagcaggta gtaataagaa ccgccgcccg
      301 gctcttccag gtaaaagaga taatccacgt atctggcctt gacttcgttg ctgttattga
      361 caccgttcat tgtcagatat acatagaggc ccagcgccgc tacgatcaat acgggcacca
      421 ggatcaacca cagcatcctc ggaccacgac gtctcttcgg tttaatcaga gacataatcg
      481 gcacctccaa tacagtaatt ccatgccaat acggtttcgg gaagtagata cttctccgag
      541 ttcaacgacc attgtatctt caatcttatc acgttgagaa aagatttttc cagatcactt
      601 ttcgcctgct ctctgaccct ggccgcctgg gtgaactgcc tagtctcttc gcccatatcg
      661 gcgatcatca atatcttcgc cagccgcgga agggagtgat agccgcttac atgccagtat
      721 atcgattcga agatatcgcc atcgatcccg tactcgttcc tgatatgaga agccgccact
      781 tttccgtgga gtagtacggg ttgcttaatt tcaagaatac acggttcgag gccgtaaacg
      841 ttggccagtc tcaacagctc gcctccggga aggtctctga aaatatcgtg tcccagagcg
      901 gccagcgagg ccttttcttc atcctcgccg tgtagtctgc tcagttccac cgcgagtctc
      961 tcgacgccca tgatgtgctc gaacctggat acggtgcaga gagctctcgc ctttttgacg
     1021 agatctttgg ctacatggga attagacccc attattctta ccgcctttct gaagaaaccc
     1081 gcatccattg aatccgtcca taaactttga actaaccgcc atcaccagga ctcgccactc
     1141 tcccctatga cttcgaagcc gttccagagg attctctcat agacagagtt gaagagagaa
     1201 tctagttctc cccttgtgtt ggcgactatc cctatcgtca cttcaaactc ttccctggaa
     1261 tcgtgacgtc ccgtttcgac aatggaggcg ttgaagtttt tcttgaggtc gttgaaaaca
     1321 ggtctgataa caccgcgttt ctccttcaag gaatttatac cctcgagctt tacaaggtaa
     1381 gtcatataac cgaaatgcat cacacggcct cccgaaaaga aagatatcgc tgcctttatg
     1441 acggataagg ccaaagccac gttcataaaa aaggagcccc gattccaggg ctccccgtag
     1501 aattttctgg attatttctg ctcttcaagt tcgcgctggc gttcgaccac tattttctgc
     1561 tgaacgtcgg gcggggtttc ctgataaccg ttgaatttca tggtgaagta gcccctgccg
     1621 ctggttattg agctcagctt gttggcgaaa tcgagcatct cagccagagg agcctgggcg
     1681 acgactttgg aagtaccttt tccctggggt tccattccca tggggcgacc ccttctcgag
     1741 gtgatttcac ccattatatc gccggttgct tcgtcgggaa cgaagacttc aatatccatt
     1801 atgggctcga gaatgacagg gttggcatcg gtcataccgt tcttgaaggc ctgtcttgca
     1861 gctatctgga acgacatatc ggaagaatca acgtcgtggt acgatccgta gaagagcgtg
     1921 actttcgtgt ccacgaccgg atagaaggct acgatgccct tcttcatggc ttcgactact
     1981 cccttttcga cggccggtat gtagttcttg ggcacaacgc ctccgacgat cttgtcgata
     2041 aattcgaagc cgccgccgcg ctcgttgggc tctatcctta tctgaacgtg accgtactgg
     2101 ccgtggccac cggtctgttt tttgtgtttg tactccgttt cgacggtctt tctgaccgtt
     2161 tctctgtagg ctatcttcgg cttgcccact tcaacatcga ccgagaagag tttcttgagc
     2221 ctctctatca taatgtcgag gtgaaccgag ccaagaccgc tgatgaccgt ttcgttggtt
     2281 tccgggtcgt tttcccactt gaaagtcgga tcggactcgg ccagtctcgc cagacctccg
     2341 cttatcttgt ctatgtctcc cttggatttc ggctggatag atttcgatat catgggttcg
     2401 ggcagtacgg ggagatcgag tttcatctgt ctatctttat gcgtcgccgt gtttccgaca
     2461 gcgcttttct tcaatttgct gagctttacg acatcgccga ccgtcgcttc atcgacctcg
     2521 atttctttta gaccttcggg cagtatcacg tgaccggcct tttcctggga atcctgatct
     2581 actacgacga aagtgtcacc ctgttttaga cttcccgaca gaagcttcat ataggtgagc
     2641 tttccaacga aaggatcgac gacggacttg aaaatatagg ctaccagtgg ctcttcttcg
     2701 gtcgggttga cctcgatttt atcatccgaa agaagcgtag cgctccttgg agaactttcg
     2761 gaagtctttt tgccgacgct tacaacgaaa tcgatcaact ggtggactcc aatgttcttg
     2821 agggccgagc cgagaaggac cggaactact tcgtttgcca ggtaggcctt tttcaacgca
     2881 ccaactatct cgtcgccgga gagttcctca ccttcaaggt acttcatcat gagttcttcg
     2941 tcgttctgca ctatatcttc gatcatcttc gtcctggcct cttcggccgc ctgggccagg
     3001 tcggccggga tagccgattc actgaaggtc ccgctgttgc cttcgtagat gtaggccttc
     3061 atggcgatga gatcgacaag tcccttgaag tttccttcac taccaatctg aagctgaacc
     3121 agtaccggtg ttctctcgaa ggtgtcggcg aggtcggcca gagcgtcctg ataggaagac
     3181 ctctctttgt ctagcatgtt gaagaaggcg ataatcccct taccctgttc cccggctatc
     3241 gcaccgaacc tctcggtctg tatttccatt cccgccgttg aatttatgac cgcaaccaca
     3301 ttttcacagg catagatccc gttaacggtt tcggccataa agtcggccat accaggggta
     3361 tcgagcacgt atattctgtt atctccgtga gcgaaactcg ttactcccat agagaagctg
     3421 gccttcttct ccttttctac ctcttcagta tcagttgcca ggatgccggt tctatcgata
     3481 agcttggcat tgaacagcat cgcatccacc agctgagttt ttccggagcc gtggtgtcca
     3541 atgagcgcga tatctctttt ggcatcgacc ggaatcttgt tcataatctg accacctcca
     3601 aatctatcta tttacgtgta cccattatta ctactggaag ggaaggtatt ccagaagaag
     3661 gagtgttaac aacttttccc tcaaccatca ttatagagga cttaccgcca tccagacaca
     3721 tcagggaatc gaaacctttg tccctgaaga attctatcat ttctttcatg gatagaccat
     3781 agctgctctg ctggtaaccg tcgataacta tgaaaactat ctcgccggtc tctttgacgc
     3841 ccaccagagt tcttggagcg cgaacgtcga tcaacgacga ggagtagtag ttacgttcgt
     3901 aatcgcttac cggcgcgccg tcgtggatta tcatcgggcc gccctcgacc gcagagacca
     3961 ggttctcccc gtaggagttg agaagtttga atttgacggt actgccgacg gaaacgtttc
     4021 ccgatttctg gaccgccgac ggcgagagga cgaggacgtt ggtgccctgt ctggcgaagg
     4081 agacgtaccc tttagagata actcttccgg agttctcgat aaccaggtac tggaaatcgt
     4141 cgaattcagg aagggtgttt ctgtactcgt ccgtgtagag aacgacctct cccctgtacg
     4201 gagagttgac gccggttatc tgtacggttc ttccgccgat ttcggccagg tagattatgt
     4261 ccatcctgga gacgtaaact ctacccttgt cggttacgta cagcgccggc ctgcccagcg
     4321 aaggcagccc cagtacctct ccgtttctta ttagcaatcc aatgggcagg ttctggttct
     4381 gatcgaagta tccgccgttg atcatgattt cggacttcct tcgattgagt atatcggaag
     4441 ccttttcgag cccggctatc ccgttggagg cgagctcgac ggagaaaacg aatttctcgg
     4501 gattcatttc gagataagac atgctgaacc tgcggccatc gacgtacgac tcgtaaagac
     4561 tcagagagta gctggatgcg gtttctatct ggaccgtcct gcccagtccg ctcttgtttc
     4621 ggaaaatcac ggacagccca tcgatctcgt actcgatgtc gccccagggc ttctcgaaga
     4681 ccaggcgaac gaagtctctt ccagatgata tcgaaacttc atcgtagtcc agcagcgggt
     4741 cgaccatagc tgggcgaagg gttatcgcca gcgagccgct cccggtccac cagtatgtta
     4801 tgaagtcagg aaggaccttc cggtccagcg tgattctgat gctgtcggcc tggttatcga
     4861 tcttcttgat tctcagaggc tgcgaataga gcaccagcga gtctgtttcg ctgaaaatgg
     4921 atgccctggt tataaccgag agcagttcgg aattgatgta tgcaacacca tcccttatca
     4981 gaagagcgtt atcgaattcg ttcccgaact ctactgtggc cctggaagtt ttgtgtgaaa
     5041 acgatactat cgccccggag ggcaggatca ttatcgtctc ctcctgtcgg agagactggg
     5101 aaatcacaac acctttcagt attttgtcga tgatttcgat gctcacaaat tcgccggaag
     5161 gagtcgagat gacgacatcg tcgtaggtca tcatctgtcc tctttcgtcg agataaacga
     5221 ggtttttcga aagggcggtg agacaagcaa caatgaaaag gagaaggaat atacgtctca
     5281 ttggcgcctc agagaacctc gtgcacggtc ggaggaggca ctacctcgcc agaggaaaaa
     5341 gaaaaggtct cggtaagggc cttcttctcc aactctacca gttcccgact gttcccgtgt
     5401 attatggcca gcgtttcacc tctggctact ctgtcgccga tcttcttcac taattccacc
     5461 cctacgaccg gatctatcac atcctcttta gtctttctgc ccgctccaag cctcatgcag
     5521 acaagcccga caccttctgc atcgattccg gagacgaagc catcgcgttc ggccttcact
     5581 tcttcgataa taccggctgg ggaaagatat ttccggtagt tggtggagaa gtctgtcgga
     5641 cctccttgag cagccacgaa cccgtcgaat ttggccaggg cttttcccga gtcgatcgcg
     5701 tccttaacta taccgggcgc gctctgttcg tcggctcttc ctcctattat caacattctt
     5761 tcggagatca cctggcacag cagttcaaaa tctgccggac cgttgccctt cagcgtctct
     5821 atagcctcga tgacctccag agaattgccc accatccttc cgagtggctg gtccatgtcg
     5881 cttatgaccg cgcctacttt tttgcctcct ctttcgccga tgtcgatcat ggccagcgct
     5941 agctcccttg catcttcgaa gttcttcatg aaggcgcctc tgccggtctt gacgtcgaga
     6001 agtattccat cggaatcgat ggcaagtttt ttactcatta tgctcgaggc tatgagcgag
     6061 atctcttcga ccgttgccgt gacatctctg agagcgtata tcttcttatc ggcaggcgcg
     6121 acttcggcag tctgaccggc gatggctatt ccgattctgg aggcctgtct cttgaatcta
     6181 tctgcatcca gcgaagttgt gaagcccggg atcgactcga gtttatcgat cgtacccccg
     6241 gtgtggccca gacctctgcc ggagagcttt gcgaagacca gcccgcagga ggccaccaca
     6301 ggccccacga cgagggtaac cttgtcgccg acgccaccgg ttgagtgttt atctaccttg
     6361 atcccctcga ttgatgacag atctattctc tctccggagt tcagcattat atgggtcagg
     6421 taggatcttt cacccgggga gagatgattg aagtaaacgg ccattaacca ggctgtcatc
     6481 tggtagtcgg gcaccgaacc tttcacatag cccattacca gttcttctat ttcggaccgg
     6541 gtgttttcca gtccgttgcg ctttttcagg atgatatcgt atattctcat tcaaacacct
     6601 tcttcaacgg cagttttacc ttgaaaatcg tacccgttcc atactcgctc tccaggtgga
     6661 tgctacctct gtgtttttct actatgaagc gagttatggc cagcccgaga ccggttccac
     6721 ccatcttcct tgatcgagcc ttgtcgactc tgtagaacct ttcgaaaata tgttttagcg
     6781 attcttcggg gatcccgaca ccgctgtctt ctacttcgat cagggcgaaa ttgttttgcg
     6841 cataaattct cagccagact tctttcgagc cgtgctcttt ggcagcggtg tatttgactg
     6901 cgttgtccac caggttcaga agtaactgaa caagtctgtc gaaatctcca tctatgaaga
     6961 tgccctcttc cagatcggtg ttcagttgca cttctctttc gtcggcaagc ggtttcacta
     7021 ttctgagaac gtaattggcc acctctacca gttcgacttc ttcgaaattg aaggccgcat
     7081 cacccgattc aagtttttcc agatcgagaa gatcgtttat caatctcgtc attctcgccg
     7141 attcgttctc gattatcttc agaaatctgt aaacggtttc tctgtcctcg agatcgtctt
     7201 cggccagcgt ctcggagtat ccgtgtattg aagtaagcgg cgttctcaac tcgtgcgaga
     7261 cgttggccac aaagtctctt ctcatcgctt ccagttcttt ctctttggtt atatctctca
     7321 gcaatattaa gtgcttcacc tgttccttca gagcaacgcg gatgatcttg cattccttta
     7381 tcgctctctt ggggtagtac atatttattt ccgattcctg gttttcgcca gtcctgataa
     7441 cctcgtcgag cagatctcca atataatagt tgtctatgcc ttcgcttatc ttccttccca
     7501 ggagttcttt gctggagaga tctctggccg atttgttcac atattcgatc ctcccgtcgt
     7561 cggaagttat taagagcgct tcgccgaggt tatccaggat ggtcatcaaa ttctcctgtc
     7621 tcgcgctgag ccggttgtaa gcctcccggc tctgggcgag cagttgttga actcttttgt
     7681 atattaaaat gaactcggcg ctttgtggaa catccaccat gtccgcaaag cgccttttag
     7741 cagcttcagc tcgttcgttc ttctgcttct cgacaaaaaa aagtataaag agagaaacag
     7801 ctacaattgt aaaaagtaga cagagaagga gcaatgaatc agtcttcctt tcccgggtct
     7861 ctgaacttgt aaccttttcc tctcacggtt attatgtact ttggattcga agcatcttct
     7921 tctatcttgg ttctgagcct tcttatgtgc acgtccaccg ttctggtatc gccgtagtag
     7981 tcgtagcccc acagcttgtc gaggagaacg tccctactga agacctttcc ttcgttctcg
     8041 gccaggaatc tcaaaagttc gaactccagg ggcgtcagac ttaccattct gtttctcacc
     8101 ctgacttcgt acttctcggt atcgatttcg agttctctgg cgattatctt cttgggtctt
     8161 tcttccttgg cctgggcgct ctgctgaatt cttctgaaaa ccgccctgat tctggccagg
     8221 acctccctta cactgaaagg ctttgtgatg taatcatcgg cgccgagttc cagtccgagg
     8281 actttgtcga actcctcgct ttttgcgctg aggaagatca ccggtgtctg tctgaatttt
     8341 tctgtagctc tcaagtttct taccagctcg aagccatcca tcccgggaag cattatatcg
     8401 actatgaaca tgtccgtttc gttgtcttcg gcaatcttta aggcttcttc cgcgtcgtag
     8461 gctttcagaa cgtcgtaacc ctccttcttc aggttaaagg tcaagagttc cacaatcgag
     8521 ggttcatcat caataacaag aatactcttc tttgccatgc atcttccact ccttccgtct
     8581 caaattcagt tcctctacat atctctggag gaaaggaata gcatttctct cctctttcag
     8641 atattccagg aaattctcag aacctgcctc atcgatgttt cctagtgcgt actgcagaga
     8701 tgattcctta agactttcaa aatccgattt tacaccgggc attctcaatt tgcttctgag
     8761 ttccctttcg atcttcttca gtgactgcaa gttttccatc ggaagttctt cgaaaggggt
     8821 tcccggtttg ggtacgaggg gattcaagct caacaccacc tgcgaataac ccatacccac
     8881 ggccaatttc gcgatctcca ctattccact cctgtcggac tcgttttcga aggaagctcc
     8941 gtatatgaaa tagagtttga tgtttttgaa tccttttttt atgcccattt ccagagcaga
     9001 ttctatgtct ctgtcgttta tacctttccc gaagaggttt cttatcctct gacttccccc
     9061 ttcgggcgct acggtgaaaa ccttctgttt accttttttt aaaacttcca gaaggttttc
     9121 cgacagagcg tccagtctca acgaagagac cgacaggtcg taaccgagtt cgagggagcg
     9181 ttccagtaaa cggtcgatga acgggtagtc ggtcaccgtt gccgcgacca gtccgaacct
     9241 tttcgtgatg cgtgcttggc gctccattat cgccagaagt tcttcaagat ctctgaatct
     9301 ggctttacca aagcgataac cagaaacaca gaatctacat tttcttgcac agc
//
LOCUS       DAOOLY010000073         9313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8629_length_9313_cov_4.918233, whole genome shotgun sequence.
ACCESSION   DAOOLY010000073 DAOOLY010000000
VERSION     DAOOLY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8629_length_9313_cov_4.918233"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2679)
                     /locus_tag="PLD15_05185"
     CDS             complement(<1..2679)
                     /locus_tag="PLD15_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63330.1"
                     /translation="MSKKVLVLLLASALAFGGLLLAVEKGAISDIIYFNVKMSQELGL
                     KDVSEGLTDIFMEGVSAPTLMGMDQATREKLDIYAVPSLTYSLFINPYPNKAPYFANI
                     AGKDFFNVMAINEIRFALNDLINRQYIVDEILGGAGGPMLSMATPGQPGTYRYGLVAN
                     KLGLSFEGNESKALEAIVKALEDASKLSENQGRLTRKDGKWYFDSDPVLVKFVIRADD
                     PNVRVKIGEYVALQLEKAGITVERLIWERTKANNVVYGSDPAEMQWNLYTEAWSAGAT
                     RAFWEHIARQMYSTGGTYMPGRGNADFWNFSNFELDELIEKAYTGNFLTEEEYWDLSL
                     TAVEMGLKDAVRLYVCYQEAYFTANKARMVDRFAYGLGDGLNEWSLKTALTQDKVLFV
                     TQFSSIGSLFMSSWDPIGPDGMSDTYTSNVVTILSDPSSFESPASAINTPFRVSWKAE
                     DITTKVERNAEGVVVGTIDVPENAIRFDSATKQWKEVGPGVKAMSSGTYTLLGGKMHN
                     GQPITVNDYLYAYAFTDEWMSKDGENDKYYEASYENYMRSSQDTILGWVVNDDNTITT
                     YFNYNFPASVDRTAQWGAPSLGVHRQPFVIVSWEIYEALAEIVALGSSSGTVYAFRND
                     SAITEVDVLVPNHVKDIRAKLVELRDKGHVPNSLKGLITAEDAKTRYQAAIDFIDRYG
                     HAFISNGPYYLSKYDSTANYAECRAFRDPSYPYESTYWKEYLKTVRLSIDSIDIPAII
                     MKGMDTTVLVNVSEVAYPSEEVSKASKGSVVVTIITPDGEINFNAELKEEGVFETVIK
                     AELLEGLEAGSYNVLAIASLEGFNLTYRELNEQTNTETDRKHQLEAAIATEEAAIEQE
                     KLGFIEKEKALQGMQHAFNDLLQVLRSKENDKNLAAQ"
     gene            3422..3850
                     /locus_tag="PLD15_05190"
     CDS             3422..3850
                     /locus_tag="PLD15_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB63331.1"
                     /translation="MERQINDIRDLIQEIAWHFGDHEFNGECCGGLTFVEFMALKKID
                     ESEYLSIQEVGRALNFTKSGATRLVNRLQTKKYIKREQSSKDGRVCCITLSSEGRNAL
                     NAIAGKYGEYLEKVLRELDQEQTELLGRILQLLANITKRE"
     gene            4000..4161
                     /locus_tag="PLD15_05195"
     CDS             4000..4161
                     /locus_tag="PLD15_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63332.1"
                     /translation="MTTIRTPRTMYHQDVDVPISLPARDAVTPRTVNVTAAPKAKTIE
                     SKNARFVLL"
     gene            complement(4231..4914)
                     /locus_tag="PLD15_05200"
     CDS             complement(4231..4914)
                     /locus_tag="PLD15_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HPB63333.1"
                     /translation="MGGFVLSLASALWFGILTSISPCPLTTNIAAVSFISRNIGSPAK
                     SMAAGIVYTFGRVLTYVVIGAVLVSSLTAAPWLSRILQKYMGKLIGPIMIIVGMFLLE
                     LLSFRNRGRNLDSRSQEKIKSKGLFGAFALGSLFALTFCPVSAALFFGSVIPLAVGEQ
                     SRLLIPAVYGIGTGLPVIIVAFGIAVGTKLVASMFKRMSQFEYWARTVTGTVFIILGI
                     YLSIERIFL"
     gene            complement(4915..5400)
                     /locus_tag="PLD15_05205"
     CDS             complement(4915..5400)
                     /locus_tag="PLD15_05205"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPB63334.1"
                     /translation="MNLKKLATFSLLLFVVVSVAYFVYDEIRLNSLVQKPEAGSTDSG
                     DTSLIEDYVTLYYFHGTIRCQTCLDMESNAKKVLETYFSSRVENGTLRWQVVNIDLPE
                     NYHFVDEYIIMYNTLVIQSYKNDEAGEWKELWEAWDLSEKGERYIQYVRDEVASFLEV
                     N"
     gene            complement(5422..5565)
                     /locus_tag="PLD15_05210"
     CDS             complement(5422..5565)
                     /locus_tag="PLD15_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB63335.1"
                     /translation="MRLAFFSDIHGNLEALEAVLEDMEKRGIDRCYCLGDLVGYGPRE
                     YLF"
     gene            complement(5562..5888)
                     /locus_tag="PLD15_05215"
     CDS             complement(5562..5888)
                     /locus_tag="PLD15_05215"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB63336.1"
                     /translation="MITLEVDLVGVLKSLSDSTNLKILALIKLYEELCVCEFEDLLES
                     PQPTVSRHLKALTDSGLIRVRKDGRWRYYGLQEIPSFVEEVIELAVETYGIIRRERRK
                     KCGDSK"
     gene            complement(5885..6946)
                     /gene="arsB"
                     /locus_tag="PLD15_05220"
     CDS             complement(5885..6946)
                     /gene="arsB"
                     /locus_tag="PLD15_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPB63337.1"
                     /translation="MSEQKLDIFSKYLSIWVAVAMVLGALLGNILPGLAYTLGNWQIA
                     NVSIPVAIVLLFMMFPIMLKIEFREIVNVRKNSKPLLVTLIVNWAIKPFTMALISWIF
                     IKAIFGPLISTGLGNEYIAGMILLGLAPCTAMVLVWTYLAGGNINYALVQVAINDLFI
                     LFLFAPMGKLLIGVSTGFPVPFSTIFWSVVFYVAIPLVLAVMTRRLVVRSKGERYLNE
                     NLIPKFDPFTKVGLLLTLVLVFMFQGRTIIDNPLHILLIALPLMIQTYLIFYIGYFAV
                     KRLKIDYAEAAPSTFIGASNFFELSIAVALILFGMNSGAALATVVGVLVEVPVMLSLV
                     AIMKKKRKLFDFSKGGMKS"
     gene            7097..7384
                     /locus_tag="PLD15_05225"
     CDS             7097..7384
                     /locus_tag="PLD15_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPB63338.1"
                     /translation="MDRRAVRIFKALSCNWRLKILEQLSRATMCMCELDELFAIDKTT
                     LSRHVKALVDSGLITETRNGTRKELSITDKRILRILEIAGEMTGDSRAGEE"
     gene            7388..7867
                     /locus_tag="PLD15_05230"
     CDS             7388..7867
                     /locus_tag="PLD15_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB63339.1"
                     /translation="MYSIVIYIITGVLLLISLIASRKKTLLALKKAWKSFEKILPELI
                     AIMLFVGLLIALLDPGTISALLGDNSGFLGLLIASVVGSITLIPGFVAFPMAAMLLNN
                     GAGITQIALFISTLMMVGVVTFPVERKYFGTKLTVLRNSFAFGFSFLVAGLMGMILR"
     gene            7864..8397
                     /locus_tag="PLD15_05235"
     CDS             7864..8397
                     /locus_tag="PLD15_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB63340.1"
                     /translation="MKCRRYIFPLIVAVVVAIFTLIDNQTGFKALSITGSSLLEMLSV
                     IPPIFVLLGLLDVWVPREKIIKHLGEESGLKGVVLSFVLGAAAAGPLYGAFPIAAVFM
                     KKGVKFSNVLIFIGAWSTTKIPMFMFEMGALGIEFALLRLAINVPGILVMALLLEKMT
                     GKEQREAIYTQAAKLSE"
     gene            8416..>9313
                     /locus_tag="PLD15_05240"
     CDS             8416..>9313
                     /locus_tag="PLD15_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB63341.1"
                     /translation="MERDLKRELTKFGLLAAGFLVFYFLPFSEKHVQEAIIGGFTMLG
                     SYAREHVLFCLVPAFFIAGTITVFIRKDAILKLLGPGARKIISYPVAAVSGGILAVCS
                     CTILPLFGGIYKRGAGLGPAVAFLFSGPAINVAAIFLTGNAIGWEMAVIRMVAAAISA
                     VFIGLIMQSIFKEKGSGGFAIQESGEQLSWQEAVGFLALQMAFLIVGGLKIDPIVKAS
                     IMTVSAVAVFIMAIHFKREMRNSWIGETWDFAKKILPYLFFGVFAAGVISIALPQSFV
                     ESLLGGNRFGSTLFASVFGAVMY"
BASE COUNT         2321 a         2258 c         2279 g         2455 t
ORIGIN      
        1 ctgggcagcc aggtttttat cgttttcttt cgagcgaagc acttgcagca agtcattgaa
       61 agcatgttgc atgccctgca gcgctttctc tttttcaatg aaacccagct tttcctgttc
      121 aatcgccgct tcttctgttg cgatggccgc ttctaactgg tgcttgcgat cggtttcggt
      181 atttgtttgt tcattcagtt cgcgataggt caggttaaaa ccttccagcg aagcaatggc
      241 gagtacgttg taagaaccgg cctcgagtcc ttcgagcaat tcagccttga tgaccgtctc
      301 gaagacgccc tcttccttca gttcggcgtt gaagttgatc tctccgtccg gtgtgatgat
      361 cgtcactacg accgatcctt tggaagcttt ggagacctct tcggaaggat acgcaacttc
      421 cgaaacattc accagaacag tcgtgtccat tcccttcatt attatcgcgg ggatgtctat
      481 ggaatctatc gacaatctca cggttttcag gtactccttc cagtaagtgg attcgtacgg
      541 gtacgaagga tccctgaagg ctctacattc agcgtagtta gccgtagaat cgtatttgga
      601 aaggtagtac ggtccgttac tgatgaaggc gtgcccgtac ctgtcgatga agtcaatggc
      661 tgcctggtat ctggttttcg catcttcggc agttatgagt cccttcagag agttaggtac
      721 atgcccctta tcccttagtt cgaccagttt ggctcttata tcctttacat ggttcggtac
      781 caggacatcg acttcggtta tcgcagagtc gtttctgaag gcataaaccg ttcctgagct
      841 gcttccaagg gcgacgattt ccgcgagggc ttcgtatatt tcccaggaaa cgattacgaa
      901 cggctgtctg tgaacgccga gcgatggagc gccccactgc gctgttctgt caacgctggc
      961 cgggaagttg taattgaagt aggtggtgat agtgttgtcg tcatttacaa cccagccgag
     1021 aatcgtatcc tgcgagcttc tcatgtagtt ctcataggaa gcttcgtagt acttgtcgtt
     1081 ttctccgtct ttgctcatcc attcgtctgt gaacgcgtaa gcgtagagat aatcgttcac
     1141 ggttatcggc tgaccgttgt gcatctttcc accgagtagg gtgtacgtac cgctagacat
     1201 cgccttcacg ccgggaccga cttctttcca ctgtttggtt gcgctgtcga accttatggc
     1261 gttttccgga acgtcgatcg tccctactac aactccttcg gcgtttcttt cgactttcgt
     1321 tgttatgtct tctgctttcc agctcactct gaagggagtg ttgatagcag aggccgggct
     1381 ttcgaaagaa ctcggatcgg agaggatcgt tactacgttg gaagtgtacg tgtcgctcat
     1441 tccatcagga ccgatcggat cccaggaaga catgaacaga gaacctatcg aggagaactg
     1501 tgtgacgaag agaaccttgt cctgcgtgag agcggtttta agagaccatt cgttaagacc
     1561 gtcgccgaga ccgtaggcga atctgtcgac cattctcgct ttgttagccg tgaagtaggc
     1621 ttcctggtaa cagacgtaga gtctgacagc gtctttgaga cccatctcta cggctgtgag
     1681 ggagagatcc cagtactcct cttcggtaag gaagttgccc gtgtaagcct tttctatgag
     1741 ttcatcgagt tcaaagttgc tgaaattcca gaaatcggca ttccctcggc cgggcatata
     1801 ggttccaccg gtggagtaca tctgtctggc gatgtgttcc cagaaggctc tcgtcgcacc
     1861 ggccgaccat gcttcggtgt agaggttcca ctgcatttct gccgggtcgc ttccataaac
     1921 gacgttgttc gcctttgttc tttcccagat caatctttct acggtgatgc ccgccttttc
     1981 cagctgcagg gcaacgtatt cgcctatttt gaccctgacg ttggggtcgt cggcccttat
     2041 aacgaatttc acaagtaccg gatcgctgtc gaagtaccac tttccatctt tcctggtcag
     2101 tcttccctga ttctcggaaa gcttcgaggc atcttcaaga gccttgacaa tagcttccag
     2161 agccttcgat tcgtttccct cgaagctcaa tccgagcttg ttggcaacca gaccgtacct
     2221 gtaagtgcca ggctgtccgg gagtagccat cgagagcatt ggaccaccgg ctccgccgag
     2281 gatctcgtcg acaatgtact gtctgttgat cagatcgttg agagcgaatc tgatctcgtt
     2341 gatggccata acgttgaaga aatccttacc cgcaatgttc gcgaagtaag gggctttgtt
     2401 tgggtacggg ttgatgaaca gggagtaggt cagcgaggga acggcgtaga tatcgagctt
     2461 ctcgcgtgta gcctgatcca tacccataag cgttggtgcc gagacgcctt ccatgaagat
     2521 gtctgtcaaa ccttcggaaa cgtccttaag accgagctcc tgagacatct tcacattgaa
     2581 gtaaatgatg tcggaaattg cgcctttctc gacggccaac agcagaccac cgaacgccaa
     2641 ggcgcttgca agtagcagta ccagaacttt cttactcaat ttgtttcgcc cccttttgca
     2701 gttagttctg tggtcgttcc acagaacgtc ttaaatcgtg tttacctatt aaaaataatt
     2761 ctcatcacga cttattttta tcaatataga ccggtactat ttgggaatta tttctttatc
     2821 tcaaaattat tttcctattc ccggatatat atcaaagtaa tatgaagttt atcacaataa
     2881 ttcgaagaaa gtcaagcaaa ttactgaagt atatctcgat atactttcta attattttgc
     2941 atgcactatc attgtactcg taattttggc cgtataggaa gtattatcag gaattttgaa
     3001 ggaaatttcc gtgtttatcg tacaatagct gcaaattgct accgtataat tcatatattt
     3061 cctttatagt caccaggaag taggtggaag atctattttc tgtacgcggt ttttctagtc
     3121 ggaatttgaa gagccatctg gtctccatgg agagcaaaag ctatatttac cccttcactc
     3181 gagcggagat taagatcaag aacatcacta actggcctgc acatggttga gaaaactttt
     3241 atagcgtttc atgattcttt tagaagtcat gaggtcagat tcatttctct gttattttca
     3301 tgtacatcgt gaggcgaaag aaaactggaa aaatttcaca ttactatagt tgactttatc
     3361 aactattttt cgatgtaata ttggttgaca atatcaacta tactcaaagg ggtaataatg
     3421 aatggaaaga caaataaatg atatacgtga tttgattcag gaaatagcct ggcacttcgg
     3481 agaccatgaa ttcaacggag agtgctgtgg aggtctgacg tttgtagaat tcatggcatt
     3541 gaaaaagata gatgagagtg aatacctgtc gatccaggag gttggaagag ccttaaattt
     3601 tacaaaaagc ggagcgacac gactggtaaa ccgtttgcag acaaagaagt acatcaagcg
     3661 cgagcaatcc agtaaagatg gaagggtttg ctgcatcact ttatcgagtg aaggaagaaa
     3721 tgctctcaat gccattgccg ggaaatatgg tgaatatctt gagaaagtgc tgagagagct
     3781 cgatcaagag cagactgaac ttctaggaag aatactgcag cttctagcaa acatcaccaa
     3841 aagagaatag ttcgtttgat tagaccaccg agcatacgga agaaccctaa aactgcgaga
     3901 atacccgcaa tgaatgctct gaaaaaacct ctcttagtat ttttggagat caagtagctt
     3961 gatagaatta tttcgaaaag accccacgtc tgcaaagcca tgaccaccat cagaacaccc
     4021 aggactatgt accaccagga tgtcgatgta ccaatcagtc ttccggcaag agatgctgtc
     4081 actcccagaa ctgtgaatgt taccgctgcg ccgaaggcaa agactattga aagcaaaaac
     4141 gcccgctttg tgctcctctg agctgtacct ccaacataac cgaacggacc gaagatgaat
     4201 tagctagaaa tgaccggcaa tcgttcttta tcaaaggaat atcctttcta tgctgaggta
     4261 tattcccaag attatgaaaa ccgtgcccgt gacagttcgg gcccagtatt cgaactgact
     4321 catccgtttg aacatgctgg ccaccagctt cgtgccgacg gctattccaa aagcgacaat
     4381 tataacgggc aaccctgtgc cgatgccgta gacggccggt atcaacagtc tcgactgctc
     4441 gccgacagcc aggggaatca cgcttccgaa gaacagcgcc gccgataccg ggcagaaggt
     4501 caaagcgaac agcgaaccca gggcgaaggc accaaaaaga cctttgcttt tgatcttttc
     4561 ctgagaccgg ctgtccaggt ttctaccacg gtttctgaag ctgagcaatt ctagcagaaa
     4621 catacccacg attatcatga tcgggccgat caatttgccc atgtattttt gaaggatcct
     4681 cgatagccat ggtgcagctg tcaggctcga tacgagtacg gccccgatca cgacgtaggt
     4741 caggactctg ccgaatgtat aaactatccc cgcagccata gatttcgccg gactgcctat
     4801 gtttctactt ataaaggaaa cggcggctat attggtggtg aggggacagg ggcttatcga
     4861 ggtcaatatt ccaaaccaca gcgccgatgc aagagaaaga acgaagccac ccattcagtt
     4921 cacctccaga aaggaagcta cctcgtcccg gacgtactgg atgtaccgct cccctttttc
     4981 ggaaagatcc caggcctccc agagttcttt ccattcaccg gcctcgtcgt tcttgtagct
     5041 ttgaatcacc agggtgttgt acatgatgat atactcatca acaaaatgat aattttccgg
     5101 gagatctata ttcaccacct gccatctcag ggtaccgttc tctacccggg aggagaaata
     5161 ggtctcgaga acctttttcg cattggactc catatccagg caggtctggc aacggatggt
     5221 cccgtggaag taatagagag ttacataatc ttcgatcaga gatgtatctc cggaatcggt
     5281 cgaaccggct tcgggctttt gaaccaagga attgagcctt atttcatcgt acacgaaata
     5341 ggcgacgctc acgacaacga aaagcagtaa agaaaatgtg gccagcttct tcaaattcac
     5401 ttttcacccc cagcgtgatt ttcaaaagag atattcccgc ggaccgtaac cgacgagatc
     5461 gccaaggcag taacacctgt cgatgcctct tttctccatg tcctccagta cggcttcgag
     5521 agcttctaaa tttccgtgta tatccgaaaa gaaggctaac ctcattttga atcaccacat
     5581 tttttgcgtc gctctctcct gatgattccg tacgtttcca ccgccagttc gataacctcc
     5641 tcgacgaaag aagggatctc ctgcagaccg tagtatctcc atcttccgtc tttccgcacg
     5701 cggatcaagc cggaatcggt cagcgctttc agatgcctgg aaacggttgg ttgcggcgat
     5761 tcgagaagat cttcgaactc acagacacat agctcttcgt atagttttat gagcgcgagt
     5821 atcttcagat tcgtcgagtc ggaaaggctt ttcaggactc caacgagatc gacttcaaga
     5881 gttatcaaga cttcattcct cccttcgaga aatcgaaaag ttttctcttt ttcttcatta
     5941 tagccaccag tgaaagcata acaggcactt caaccagaac cccgacgacc gtggcgagtg
     6001 ccgcgccgga attcataccg aagagtatga gggccaccgc tatcgagagt tcgaagaagt
     6061 tggatgcccc tataaaagtc gatggcgcag cttctgcata atctatcttc aaacgcttta
     6121 cggcgaagta ccctatgtag aagatgagat aggtctgtat catcaaggga agggctatca
     6181 gcaaaatgtg gagagggttg tcgattatgg ttctgccctg gaacatgaag accagcacga
     6241 gtgtaagtaa aagtccgacc ttcgtgaagg gatcgaattt tggaatgagg ttttcattca
     6301 ggtacctctc ccccttagac ctcaccacca ggcgtcgtgt cataactgcc aggacaagcg
     6361 ggatagcgac atagaagacg acagaccaga agatcgtgga gaatggcacg ggaaatcccg
     6421 tggatacgcc aatcagaagc tttcccatcg gggcgaagag aaagagaatg aaaagatcgt
     6481 ttatagccac ctgaaccagc gcataattta tgttgccgcc ggcaaggtaa gtccacacaa
     6541 gcaccatggc cgtgcaggga gccagtccga gcagtatcat gcccgcgatg tattcgttgc
     6601 ccaatccggt agaaatcaga gggccgaaga tcgcttttat gaatatccag gagatcagtg
     6661 ccatagtgaa cggcttaatt gcccagttga caattagcgt taccagcaga ggcttcgagt
     6721 tttttcggac gttgacgatc tccctgaact ctatcttgag catgatcgga aacatcatga
     6781 agagaagaac aatagccacc ggaatcgaca cgtttgctat ctgccagttc ccaagcgtat
     6841 aggcaagccc cggtaaaatg ttgcccagca gagcccccag taccatcgcg actgccaccc
     6901 agatcgagag atacttgctg aatatatcga gcttctgttc cgacatattt tcctccacaa
     6961 tattcatata ttcgaatatg cgaatatgat acccgaataa cattttgaaa gttttctcgg
     7021 agtgtaaaat ttcattgttg cacaacaatg caacttgagt tattcttgag attggaaatt
     7081 cgacaggagg tgccagatgg acagaagggc tgtaaggatt ttcaaggcgt tgagctgcaa
     7141 ctggcgtttg aagatactcg agcagctgtc gagagcaact atgtgcatgt gcgaactcga
     7201 cgagcttttt gcgatagaca agaccacact ctcgcgccat gtgaaggctc tggtagattc
     7261 cggactcatc accgagacca ggaacggcac gagaaaggaa ctgagcatca cggacaaaag
     7321 gatactgcgt atactcgaga tcgcaggaga gatgaccggc gattcccgag ccggcgagga
     7381 atagaagatg tacagtatcg ttatctatat aataaccggt gttctgctct tgatttcact
     7441 catagccagc cgcaaaaaga cgttactcgc cttgaagaag gcctggaagt ctttcgagaa
     7501 gatactgccg gaactcatcg ccattatgct cttcgtcggg cttttgatcg ccctgctcga
     7561 tcccgggact atatcggctt tgctcggaga caactcgggg tttctcgggc ttctgatcgc
     7621 ctcggtggtt ggatccataa cgctcatacc gggctttgtg gccttcccga tggccgccat
     7681 gctgctcaac aacggagccg ggataaccca gatcgcgttg ttcatttcca cgctaatgat
     7741 ggttggcgtg gtaacctttc cggtcgagag aaagtatttc ggaacgaaac tcactgttct
     7801 tagaaattcc ttcgccttcg gcttctcttt cctggtggcc ggattgatgg gaatgatctt
     7861 gagatgaagt gcaggagata tatctttccg cttatcgtgg ccgttgtggt cgctatcttc
     7921 acactcatcg acaatcagac agggttcaaa gccttaagca taaccggttc aagccttctt
     7981 gaaatgctct cggtcattcc tcccatattc gtgcttctgg ggcttctgga tgtctgggta
     8041 ccgcgcgaga agataataaa acatctggga gaggagtcgg gcctcaaggg cgtcgttctg
     8101 tctttcgttc tcggcgcggc cgcggccggc cctctctacg gcgccttccc catcgcggcc
     8161 gtcttcatga agaagggcgt caagttttca aacgttttga ttttcatagg tgcctggtcg
     8221 acaacgaaga tccctatgtt catgttcgaa atgggcgctc tgggtattga attcgctctc
     8281 ctgaggctgg cgataaacgt tccagggata ctcgtaatgg cgttgttgct ggagaagatg
     8341 accggcaagg aacaacggga agccatctac acacaggcag ccaaactcag cgaataaatc
     8401 agggggtgct ccgggatgga aagggatctg aagagagaat tgacgaaatt tggactttta
     8461 gcggcgggtt ttctggtttt ttattttctg cccttctcgg agaaacacgt gcaggaggcc
     8521 ataatcggcg gtttcacgat gctcggctct tatgccaggg aacacgtgct gttctgtctc
     8581 gttcccgcgt ttttcatagc cgggacgata accgtgttta tacgcaaaga cgcgatactc
     8641 aagctgctgg gtccgggtgc tagaaagata atatcctacc cggtggcggc cgtgtcaggc
     8701 ggaatcctcg ccgtctgttc ctgcacgata ctgccgctct tcgggggaat ctacaaacgg
     8761 ggagccggcc tcggcccggc cgttgccttc ctattcagcg gcccggctat caacgtggcg
     8821 gccatatttc tcaccgggaa cgcgatcggc tgggagatgg cagtcataag gatggtagcg
     8881 gcggcgattt cggcagtttt catagggctc ataatgcaaa gtatcttcaa agaaaaaggt
     8941 tcaggtggct ttgccatcca ggaatcgggc gagcagcttt cctggcagga ggccgtaggc
     9001 tttctcgccc tgcaaatggc tttccttata gtcggcggcc ttaagataga tccgatcgtt
     9061 aaggcttcta taatgaccgt ttcggccgta gcggtcttca tcatggcgat tcatttcaag
     9121 agagagatga ggaactcctg gataggcgag acctgggatt tcgctaagaa gatacttcca
     9181 tatctcttct tcggagtctt cgcggccggc gtcatctcca tcgcacttcc tcagagtttt
     9241 gtagaatctc ttctcggcgg aaacagattt ggttcgactc tgtttgcttc tgttttcggt
     9301 gcggtgatgt act
//
LOCUS       DAOOLY010000074         9275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8682_length_9275_cov_5.862690, whole genome shotgun sequence.
ACCESSION   DAOOLY010000074 DAOOLY010000000
VERSION     DAOOLY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8682_length_9275_cov_5.862690"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..952
                     /gene="ruvC"
                     /locus_tag="PLD15_05245"
     CDS             452..952
                     /gene="ruvC"
                     /locus_tag="PLD15_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPB63342.1"
                     /translation="MSDPVRVLGIDPGFGTLGYGIIEARASRVDLVAYGAIKTESGEP
                     IPDRLLKIYDELEAITRKYSPHESAVEELFFFRNVTTAIEVGEARGVILLVLRRLCVP
                     IYEYTPHQVKMAVTGYGRAEKGQIQRTLKAFLGMSETPKPDDAADALAVAWCHISSRR
                     FPGGVR"
     gene            949..5187
                     /locus_tag="PLD15_05250"
     CDS             949..5187
                     /locus_tag="PLD15_05250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPB63343.1"
                     /translation="MKLKIREINLPASRIIENVLGFYPDCQCEDSTLSDIILNPGKKE
                     ITFIFCGKTPDDLKDFLRTKFQKIASAQLKSKIQLTFRENTTEEPAGPSSRVAEFDPR
                     EILKWTNGASSYLSSSRIEYDGNKITITVGNHFSLQRINSKKADIRMAIEKVAGTAIP
                     FEITLEEMPEAEAEALAEAVILEREIEKQPESQPAIVERILLGREIKAVSRTMSEVIG
                     NESDLVVSGKVFGLEFRNNRFPVLMFNLTDHTDSLCVRITREDAKNLSTRLEDGDEIT
                     VRGKMETDTWLKEDVLVPRDIVRTSLARREDNAPVKRVELHLHTKMSALDSVVETKNL
                     IKTLKEWNHDAVAITDHGIVQSIPEFYFEAKKAGIKAIFGMEGYMINDSEPILYNLQD
                     EALSLEEASFVVFDLETTGLNPKEDEIIEIGAVRVEGNEIIDTFSSFVKPSKEVSSLI
                     TSITGITNEDLESAQDIKTVLPGFIKFCEGSILVAHNALFDYGFVRNTVKKHFGGDWE
                     MPCIDTLALAKSLIKSKSYSLDTIVKKLKLGHFEHHRASEDARVTSEVLRKFISMAKK
                     RGVEKVTELDRLRKNLNLNSLKPIHISILARNKKGLGNLYRLVTISHTEHYYMKPRIP
                     KSVLNELHEGLLIGTACISGEFARSYISGASTEELEEIAKFYDYIEVMPLDTIDSTEG
                     EANISAETLKNMYREFYKVGKKLNIPVVMTGDVHFLEPHEKIFRAAINAAQDYENFQN
                     QPDLHLHTTGEMLAKAMEIFEDEAVAREVVIDNTRYISSLIEEIVPVQGKLHPPIIEG
                     AEDEVRRLTMENARRIYGDPLPEIVEKRLEKELNAIIGHGYAVLYLIAQKMVQKSNED
                     GYLVGSRGSVGSSLVANVIGITEVNPLPPHYLCPECGNSIFPETDVESGYDLPDRFCP
                     TCSGKMKKNGQNIPFETFLGFEGDKVPDIDLNFSGEYQERAHSFIEELFGRDHVFRAG
                     TISTLAEKTAYGFVRAYSEKSGKTFRRPEQERIARGISGVKRTTGQHPGGLMIVPKDL
                     DVHDFTPVQHPANDTKSSTLTTHFAYEVIHDDLVKIDALGHDDPTFIKMLKDITGIDP
                     EDIPMDDRDTLSIFSSVKALGIRSQELGTDMGTLGIPEFGTGFVRGMLTDTRPATFGE
                     LVRISGLSHGTDVWLNNAKDIITSGQATLSKVIACRDDIMNYLISRGMEPAQAFKIME
                     KVRKGKGLKEDDEKAMRALKVPEWFLNSCKKIKYLFPKAHAAAYVSMGYRVAYFKVHH
                     PLAFYSTYFSIKGDEFDIDLCTSDLNSIKKEILRLRAEQDRNVKDRAKETLLEVVMEM
                     LLRGFGFLNVDLMKSHPTRFEIEGKTLRIPFNRLQNMGDKAAMSIDMEREKRPFSSVE
                     DLMRRTSLNKTSIEMLRKYGALKGLPEKEQITLF"
     gene            5196..6239
                     /locus_tag="PLD15_05255"
     CDS             5196..6239
                     /locus_tag="PLD15_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63344.1"
                     /translation="MEFSINSEEYSRLIDLLNRVAGTVEPTYRYFQIYHRRGLKFFVS
                     DGCAKLEFSSPIEVDPFTGVYTVPIDYARALKPGKRDTTVTFSTTGSQIKISTGSETL
                     TLDRTRTEELPLMERKFEFSFRYSLKEFRDGLDFVSAASMEGDSIEIFSKEEKTYMAA
                     AAGKLVLVASIDSLPKYGFAFSIPYVTARHLVKAFEELKVEEIDTGVGISELGFKVGP
                     LLFSVCTDETTFSPPISLIRNSGGHRVERRELLIALGKITRLSRRGFSVLMISRNSTL
                     SFYLKAANLRYESIASSFSGGSFVVQIDPHRLHSVVSRMKAPALNLSIQEKNLLVGSP
                     ESGRYLLIPLVQP"
     gene            6279..7667
                     /locus_tag="PLD15_05260"
     CDS             6279..7667
                     /locus_tag="PLD15_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB63345.1"
                     /translation="MSGSIVISLATVTGVVDLILETRAIGKSFDGHKVLDKIDFNIRH
                     GELHVIGGLNGSGKSAFVKILTGQLERDSGSVYIEGRQVEIESFKDARKLGMAMVHQE
                     YMMVPDLTLAENIFLGIGQFLTSRKKMNQRAGAILERVGLELDPSKKALEIDRDSQFF
                     LEIATAIASKPSLLIIDDALTGLSGSLHQRALKLLREFTAEGISVLLTTVDPEIMLLG
                     DTLSIIERGRLIDVTAENAGELPVEFHPQPPVPIENRSGEAFIVVEDLADTGKSLRLT
                     KGETVCLLCESRQGMRDITRAITRQNPFRENAVPIFEEAKNFFERFARKTSSHSAYFR
                     TNYKNNGFVICQMLQKRESFKFRRSFKKGASLNREPPVSVREYLASMGLAENFLPLIT
                     TRDRELADMGFKAFDLFIFLQPSIGLDPSLLRVLNGLFQRIVEAGKIAVIISDGDGFC
                     RGLCGRVERVEI"
     gene            7664..8677
                     /locus_tag="PLD15_05265"
     CDS             7664..8677
                     /locus_tag="PLD15_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB63346.1"
                     /translation="MIPDFSYTKERIDFSSGYIFKGKHYRCSLIRYPSRYAYSAKGSE
                     NVELYHFSPRENPVGSIIILHGLGTVNIPFLFWMGSHLASAGLQATVMILPGNYTRTA
                     TGSTSGKDYFSHDLSRLVRFWEQAVVDTLSTIDFLQQEKIWWDNNCLFGYCLGGMVAT
                     IVNALEDRIKNMILMTVGGNMARIIWGSPVLKSLRGSFARGLGSEGFLNDEKRLEERF
                     KRDIHRLHEFSSVEQLIESDLHPLFKIDPLAYARFSDRDRVTMIEALFDKTLPYQTRK
                     QLWEAFKRPKRYIIPIGHVSWFPFEYALGKFILKKMGTKEARRKLRLLETIKPGIEWN
                     HRK"
     gene            complement(8932..>9275)
                     /locus_tag="PLD15_05270"
     CDS             complement(8932..>9275)
                     /locus_tag="PLD15_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPB63347.1"
                     /translation="AFCGIDCANCPAYIATYENNDRLRTETAAKWSEMFHSSIKKEDI
                     NCLGCHSGGPLFSYCKECGIRKCALEQKIKETCAECADYSCQLLTEFHKMLQEEKKIL
                     DRLHSEIEMRS"
BASE COUNT         2453 a         2437 c         2434 g         1951 t
ORIGIN      
        1 gccgcgagtt caagtctcgt cgtccccgcc agagagagtc ctgagggact ctcttttttg
       61 tttcttcatg agacggtttg tacccggttt taaacacagc ctcaacagta aagatcccct
      121 tcttcacatt cttcactgta aagagggata gaatgtaaat tggtccgtga agggttgtgt
      181 ccgattcccc ggagatttat tcaaagtgcc cgttcgccgt tagataccgg tgcgttcata
      241 aagcatcgaa aatctttgat acgaaaaagc tggcgatcag aattatgttt ttcgtattgt
      301 aaaagaaact ttgtaaaccc ggttttttct ctgtaattgg cttctggaaa gactctaagt
      361 atgtcaaacc cctgagcgtt actgccacaa agagtttgga aggcctaaac aaatttccgg
      421 cgttagaatg atagaactgg aggtgcagaa attgtccgat cctgtcagag tgctcggaat
      481 cgatccgggt tttgggactc tgggttatgg aataatcgaa gcccgcgcat ccagagtaga
      541 tcttgtggct tacggtgcga taaagacaga atccggcgaa ccgattcccg acaggcttct
      601 gaagatatac gatgaactcg aagcgattac gaggaagtat tcgccacacg aatcggcggt
      661 cgaggagctc ttcttcttca gaaacgttac caccgccatt gaggtcgggg aggccagagg
      721 ggtgatactt ctggtcctca gaaggctttg cgtcccgata tacgaataca ctccacacca
      781 ggtaaaaatg gccgttaccg gctatggaag ggctgaaaag ggacagatcc agagaactct
      841 gaaggccttt ctcggcatga gtgagacgcc caaacccgac gacgcagccg acgcactggc
      901 cgttgcctgg tgtcatattt catcgagaag atttcccgga ggtgttagat gaagttgaag
      961 ataagagaga tcaacctgcc ggcatcccgg attatcgaga acgttctggg tttctacccg
     1021 gactgtcagt gtgaagacag cacgctctcg gatataatac tgaacccggg gaaaaaggag
     1081 atcactttta tcttctgcgg gaagaccccc gacgatctga aggactttct ccgcacgaaa
     1141 tttcagaaga tagcctccgc ccagctaaag tccaagatac agctaacctt ccgagaaaac
     1201 accacggagg aaccggccgg gccgtcttcc agagtagcgg aattcgatcc gagagagatc
     1261 ttaaagtgga ccaacggagc cagctcgtac ctttcctctt cgagaatcga gtacgatggt
     1321 aacaagatca ccataacggt cggcaaccac ttctcgcttc agagaataaa ctcaaagaag
     1381 gccgacattc gtatggcgat agaaaaggtc gccggaaccg cgatcccttt cgagataact
     1441 cttgaggaga tgccagaagc cgaagcggag gctctcgcag aagccgttat ccttgagaga
     1501 gaaatcgaaa aacagccgga atcccaacct gcaatcgtgg agagaatcct gcttggcagg
     1561 gaaataaagg ccgtttccag aactatgagc gaggtaatag gcaacgagag cgacctggta
     1621 gtcagcggaa aagttttcgg gctcgagttc cggaacaacc gcttcccggt cctcatgttc
     1681 aacctcacag atcacaccga ttctctctgc gtgaggatca cgcgtgaaga tgcgaaaaac
     1741 ctctccacca ggctcgaaga cggtgacgaa ataaccgtca ggggaaagat ggagaccgat
     1801 acgtggctga aggaggatgt cctggtgccg agggatatcg tcagaacgag ccttgcgcga
     1861 agagaggaca acgctcccgt caaaagagtc gagcttcacc ttcacaccaa gatgagcgcc
     1921 ctggattctg tggtcgagac caaaaacctt atcaagaccc tgaaagagtg gaaccatgac
     1981 gccgtcgcca taacggacca cggcatcgtg cagtcgattc ccgagtttta cttcgaggcc
     2041 aagaaggccg gaataaaggc catcttcggc atggaaggct acatgataaa cgattccgag
     2101 ccgatcctgt acaaccttca agacgaggcc ctctcgctgg aggaggcttc tttcgtggtc
     2161 ttcgacctcg agaccacagg cctgaatccc aaagaggacg aaataataga gatcggcgcc
     2221 gtgagagtcg agggcaacga gataatcgac accttctctt ccttcgtcaa accgtctaaa
     2281 gaggtctctt cactgatcac cagcataacc ggaataacca acgaggatct ggagagcgca
     2341 caagacataa agaccgttct cccgggattc ataaagttct gcgaaggctc catactggtg
     2401 gcccacaacg ccctcttcga ttacggcttc gtgcgcaaca cggtcaagaa gcatttcgga
     2461 ggagactggg aaatgccctg catagacaca ctggcactcg ctaaaagcct cataaaatct
     2521 aagagttact cgctggatac catcgtcaaa aaattgaagc tcggccactt cgagcatcac
     2581 agggccagcg aagacgccag agtcacctcg gaggtcctca gaaagttcat ctccatggcc
     2641 aaaaagaggg gcgtcgagaa agtcaccgag ctcgacagac tcaggaaaaa tctgaatctg
     2701 aactccctca aacccatcca cataagtatt ctggccagaa acaagaaggg actgggaaat
     2761 ctgtacaggc tcgtgaccat ctcccacacc gaacactatt atatgaagcc gagaattccc
     2821 aagagcgttc tcaacgaact gcacgaaggt ttgcttatcg gcacggcctg tatttccggc
     2881 gagttcgcgc gttcctacat ctccggggcc agcaccgagg aactggagga gatagccaag
     2941 ttttacgatt atatcgaggt aatgcctctg gatactatcg attcgaccga gggagaggcc
     3001 aacatatccg cggagacttt gaagaacatg tacagggagt tctacaaagt cggaaagaaa
     3061 ctcaacatac ccgtcgtaat gaccggcgac gtccacttcc tggaacccca tgaaaagata
     3121 ttcagggccg cgataaacgc ggcgcaggat tacgagaatt tccagaatca gccggacctt
     3181 catctccaca cgaccggaga gatgctggcc aaagccatgg agatcttcga agacgaggct
     3241 gtcgcgcgcg aggtagtgat agataacacc agatatatat cctcgctcat cgaggagatc
     3301 gttcccgttc agggaaagct ccatccaccg atcatagagg gcgccgaaga cgaggtgagg
     3361 agactcacaa tggagaacgc cagaaggata tacggagacc cgcttccgga gatagtagaa
     3421 aaacgtctgg agaaggagtt gaacgcgata atcggccacg gttacgccgt tctctatctg
     3481 atcgcccaga agatggtgca gaaatcgaac gaagacggtt atctggtagg ttcgagaggc
     3541 tccgtcggca gctcgctggt ggccaacgtg atagggataa ccgaggtcaa cccgctaccg
     3601 ccgcactatc tctgtcccga gtgcgggaat tcgatctttc ctgaaaccga tgtcgaatcc
     3661 ggttacgacc tcccggacag gttctgtccc acctgttcgg ggaagatgaa gaagaacggc
     3721 cagaatatcc ccttcgagac ttttctggga ttcgagggag acaaggtgcc cgatatagat
     3781 ctcaacttct ccggcgaata tcaggaaagg gcgcactcct tcatagagga gctgtttgga
     3841 agggaccacg ttttcagagc cggcacgatc tccacgctcg cggagaaaac ggcttacggt
     3901 tttgtcaggg cctacagtga aaagagcggg aaaaccttca gaagacccga acaggagagg
     3961 atcgcccggg gaataagcgg tgtgaagcgc accaccgggc agcaccccgg aggactgatg
     4021 atcgtcccca aggacctgga cgtacacgac ttcacaccgg ttcaacatcc ggcgaacgac
     4081 acgaaaagct ccacgctgac gactcacttc gcctatgaag tcatccacga cgatctcgtc
     4141 aagatagacg cgctgggcca tgacgatccc accttcataa aaatgctcaa ggatataacc
     4201 ggaatagacc ccgaggatat cccgatggac gatcgtgaca cgttatccat attctcctcc
     4261 gtcaaggctc tcggtatcag atcgcaggag ctgggcacgg atatggggac tctgggtatc
     4321 cccgaatttg gaacggggtt cgtccgcgga atgctcaccg acaccaggcc ggccaccttc
     4381 ggagagctgg tgaggatatc cgggctctcg cacgggacgg acgtctggct caacaacgcc
     4441 aaagacatca tcacttccgg ccaggccacc ctctcgaagg tcatcgcctg ccgcgacgac
     4501 ataatgaact acctgatatc caggggaatg gaaccggccc aggccttcaa gatcatggag
     4561 aaagtcagga agggaaaagg cctcaaagaa gatgacgaga aggcgatgag ggcgctcaag
     4621 gttcccgaat ggttcctgaa ctcgtgcaag aagatcaaat acctctttcc gaaggcacac
     4681 gcggccgctt acgtcagcat gggctacagg gtggcctact tcaaggtcca ccaccccctc
     4741 gctttctaca gcacttattt ctcaataaag ggcgacgaat tcgatatcga cctctgcacc
     4801 tccgatttga actctatcaa aaaagagata ctcagattga gagccgaaca ggacaggaac
     4861 gtcaaggaca gggccaaaga aaccctgctg gaagtggtca tggagatgct tctcagaggc
     4921 ttcggcttct tgaacgtgga tctcatgaag tcacatccca ccaggttcga gatcgaaggg
     4981 aagacgctga ggataccttt caatcgtctc cagaacatgg gagacaaggc cgcgatgtcg
     5041 atcgatatgg agagggaaaa gagacccttc tcctccgtcg aggacctcat gagaaggacg
     5101 tctctcaaca aaaccagcat cgagatgctc agaaagtacg gagccctcaa aggactcccc
     5161 gagaaggaac agataacgct tttctgaggt ggccgatgga attcagtatc aactctgaag
     5221 agtactccag actcatcgat cttctgaaca gggtcgccgg aacggtagag cccacttatc
     5281 gttatttcca gatctaccac aggcggggcc tgaagttttt cgtgagcgac ggctgcgcca
     5341 agctcgagtt ctcctctccg atcgaggtcg acccctttac cggtgtgtac accgttccga
     5401 tcgattacgc cagggctctg aagcccggaa aaagggatac caccgttacc ttctcaacta
     5461 ccgggagcca gatcaaaata tcgacgggct ccgagacgct gactctcgac agaactagga
     5521 cggaagaact gccgctcatg gagagaaagt tcgaattctc cttccgctac tcgctgaaag
     5581 agttcaggga cggcctcgat ttcgtctcgg ccgcctcgat ggagggagat tcgatcgaga
     5641 tcttttcgaa agaagaaaag acctacatgg cagccgccgc cgggaagctg gtcctggtgg
     5701 cttccataga ctccctgccc aagtacggtt tcgccttttc gatcccgtac gttaccgccc
     5761 gccatctggt aaaggccttt gaggagttga aggtcgaaga gatagacacc ggagtcggaa
     5821 tatccgaact cggcttcaag gtcggaccgc tactgttttc tgtctgcacc gatgaaacca
     5881 ctttctctcc gccgatttcg ctgataagaa acagcggtgg acaccgggtg gagaggagag
     5941 aactcctgat agcgctggga aagatcacaa ggctctccag aagaggcttc tcggtgctga
     6001 tgatctccag aaactcgact ctcagcttct acctcaaagc cgccaatctg cgctacgagt
     6061 cgatagcctc gagcttttcc ggaggcagct tcgtcgttca gatcgaccct cacagacttc
     6121 attcggtagt ttcgagaatg aaggccccgg cactcaacct ttcgatacag gagaagaacc
     6181 tgctcgttgg cagtccagaa agcggccggt atctcctgat ccctctggtt caaccctgat
     6241 agggaattaa ttcatcgtaa aactttttag agaccgccgt gagtggtagt atagtaatat
     6301 ctctggcaac agtcacagga gtggtggatt tgatacttga aaccagagcg atcggaaagt
     6361 ctttcgacgg tcacaaggtt ctggataaaa tagatttcaa cataagacac ggcgagcttc
     6421 acgtgatagg cggtctgaac ggttcgggca aatcggcgtt tgtgaagatc ctgaccggcc
     6481 agctcgagcg agatagcggt agcgtgtaca tcgaggggcg ccaggtggag atcgagagct
     6541 tcaaagatgc gaggaaactg ggaatggcca tggttcacca ggaatatatg atggtcccgg
     6601 acctcacgct cgccgaaaat atctttctgg gtatcggtca gttcctgacc agtagaaaga
     6661 agatgaacca gagggcaggg gccatactcg agagggtcgg cctggaactg gatccttcga
     6721 aaaaggccct ggaaatagac cgcgactctc agtttttcct cgaaatagcc accgccatcg
     6781 catcgaaacc ctccctgctg ataatagacg acgccctgac gggtttgtcc ggctctctcc
     6841 atcagagagc cctgaaactc ctcagagaat ttacagccga aggaatttcc gtgcttctca
     6901 ccacggttga cccagaaatc atgttgctgg gggacactct ttcgataatc gaacgcggca
     6961 ggctcatcga tgttacggca gaaaatgccg gcgagctccc cgtggagttt cacccgcaac
     7021 ctccggtgcc tatcgagaac agatccggtg aagccttcat cgttgtggag gacctggccg
     7081 acaccggcaa gagcttgaga ttgacgaagg gggaaacggt ctgcctgctc tgcgaaagcc
     7141 gacaggggat gagagatatc accagggcga taacccgtca gaacccgttc agagagaacg
     7201 ccgtcccgat attcgaagag gccaagaact tcttcgagcg tttcgcccgt aaaacttctt
     7261 cgcacagtgc gtactttcga accaactaca aaaacaacgg cttcgtgatc tgtcagatgt
     7321 tgcagaagag ggagagcttc aaattccgcc gatccttcaa gaaaggggcg tcgctcaaca
     7381 gggagccgcc ggtctccgtc agggagtatc tggcgagcat gggactggcc gagaatttcc
     7441 tccccctcat aacgacccgg gacagggaac tcgcagatat gggcttcaaa gccttcgatc
     7501 tcttcatctt cttgcagcct tcgatcggac tggatccctc gctgctgaga gtcctcaacg
     7561 gtctgtttca gagaatcgtc gaggccggga agatcgccgt gataatcagt gacggcgacg
     7621 gcttctgcag ggggctgtgc gggcgtgtcg agagggtaga gatatgattc cggatttttc
     7681 ttacaccaaa gagagaatag atttttccag cggatatata ttcaagggaa aacattaccg
     7741 ttgctcgctg atcaggtatc cctccagata cgcctacagc gcaaagggca gcgagaatgt
     7801 cgagctttat cacttctcac ccagagagaa cccggtcggc tctataatta ttttgcacgg
     7861 cctgggaact gtgaatattc cctttctctt ctggatggga agccatctgg ccagcgccgg
     7921 acttcaggcc accgtgatga tccttccggg aaattacacc agaaccgcca caggctccac
     7981 gagcggcaag gattacttct cccacgatct ctccagactc gtgaggttct gggaacaggc
     8041 ggtcgtagat acgctctcga cgatcgattt tcttcagcag gagaagatct ggtgggacaa
     8101 caactgtctc ttcggttact gtctgggagg aatggtggcg accatcgtga acgctctgga
     8161 agacaggatc aagaacatga tcctgatgac ggttggcggc aacatggcca ggataatatg
     8221 gggctctccc gtactcaaat ccctgagagg ctcattcgcc aggggactgg gatcggaagg
     8281 ttttctcaac gatgagaagc gtctcgaaga acgtttcaaa agggatattc accggctaca
     8341 cgaattctcc agcgtcgagc agttgatcga atccgatctc cacccgctct tcaagataga
     8401 cccgctggcc tacgccaggt tcagcgaccg ggacagggtg accatgatcg aagcgctctt
     8461 cgacaaaacc cttccctacc agactagaaa acagctgtgg gaggccttca aaagacccaa
     8521 aaggtacata atcccgatag gccacgtgtc gtggtttccc ttcgaatacg ccctgggcaa
     8581 gttcatactc aagaagatgg gtacaaagga agccagaagg aaattgagac tgctggaaac
     8641 gattaaaccc ggtatagagt ggaaccatag aaaataaaaa aggccggaag cgctttcaag
     8701 cgtctctggc cttttgccgg tctatgacta aattaagcgt atatttgtaa tggctgagtc
     8761 tctcttttcc ggggcggcga aagatatgta cactacgccg ccgaactcct taaccaattt
     8821 cctcgcacga tcgaaacact ctctttccaa catggacaat acccccgttc tctcgagcct
     8881 ccggcttaac acggatattc cgccggaacc ggggagacgc gacatccccg ttcaactgcg
     8941 catttctatc tccgagtgga gcctgtcgag tatcttcttc tcctcctgaa gcattttatg
     9001 gaactctgtg agcaactggc aggagtaatc ggcgcactcg gcgcaggttt ctttgatttt
     9061 ctgttccaac gcgcatttcc ttatcccgca ctctttgcag taggagaaca aaggaccgcc
     9121 actgtgacaa cccagacagt ttatatcttc cttctttatg gaagagtgaa acatctccga
     9181 ccacttggcg gccgtttcgg tcctcaaccg gtcgttgttc tcgtaagtcg cgatgtatgc
     9241 cggacaattc gcacaatcga ttccacagaa agcta
//
LOCUS       DAOOLY010000075         9267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8694_length_9267_cov_5.335866, whole genome shotgun sequence.
ACCESSION   DAOOLY010000075 DAOOLY010000000
VERSION     DAOOLY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8694_length_9267_cov_5.335866"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..514
                     /locus_tag="PLD15_05275"
     CDS             95..514
                     /locus_tag="PLD15_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63348.1"
                     /translation="MYGWIKFLGWFNFSLLVLNSSLFITRWFYRKFNKKLQKFPWEGF
                     KNLMKFLARIHPYTGSVLLITALYHGYLATGGFVIYSGSLVFFGVLAQFIVYLLGKYV
                     PAMKKSWRPIHRLLMLVTWALFFVHVFGPYSIWIPIF"
     gene            complement(491..1474)
                     /locus_tag="PLD15_05280"
     CDS             complement(491..1474)
                     /locus_tag="PLD15_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB63349.1"
                     /translation="MKILIIGVAVLAVILLVPFLIPVPDLKGTVDPSLLADEDSRFVW
                     LENVNIHYKVSGEGFPPVVLLHGFGASIFSWREVFSPLADRFTVMAFDRPGFGLTSRP
                     IGEEIKDFNPYTPENQVELTVRLMDHLGFEKAVLIGNSAGGLTALEIAIAHPERVIGL
                     VMVDAAVYTNDSDNLFFKLLTSTPQGRHLGPLVSRSFLNNAGSLLDLAWHDPSEITPE
                     MLEGYEKPLKANNWDRALWELTLARKKFDVSKLGSINIPTLVISGDDDRIVPVEDSVR
                     LSQEIPNAVLHVFPDTGHVPQEENPQEFLEVVLPFIESLSFMEQSKDRNPD"
     gene            complement(1493..2275)
                     /locus_tag="PLD15_05285"
     CDS             complement(1493..2275)
                     /locus_tag="PLD15_05285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB63350.1"
                     /translation="MESEVNVDMELIKHAINKLDCGFLIYGVRADLLYANEAGIKLAS
                     KCEGKIKEKIEELVAREETLSGRFECSQQEQGGRSFRYFFKPLSLNGKDYFLLQIEDI
                     TDLKIAESTIAQLAHYDGETGLPNYILFRDRLNMSIFRGKRHSDLSMVAAMEIKIGSR
                     NDSNPELLIIDLAQDLSRNIRKSDTLCRFSRREFLLLAEELKRPEDAEKIMRKMGETF
                     ETWKGNIPGCSFGLSYGYSLLPVDGVDPETLVSKAFNAIKEK"
     gene            2435..4174
                     /locus_tag="PLD15_05290"
     CDS             2435..4174
                     /locus_tag="PLD15_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPB63351.1"
                     /translation="MRLIVHSGEDVFEDDFPPGTPLLSALGELGIVIDTPCGGAGKCG
                     KCRVRVDLDSMLSPPGQAEQTILGEALKRGIRLSCMAEITGDCQVYLDPGYFDAIATI
                     DEISFEGCDPVVRRQSFVIPSGDGVRNQVSALVEEYLGEDLDLSDFYTGSLEGEVSVY
                     TRPGKLVAIDGRRSFNLGVAVDIGTTTIATALLDLDSGEVLAKENTMNPQRAFGHDVI
                     SRITHTVVEREGLKTLSSVVRKAVSKLIEGLCEQSGRRSGDINEVVVSGNTTMTHLLF
                     GFPVRSIATAPFSPFYGGIVRKKAGEIGLELSPFSEVLALPAISGYVGGDIVAGILST
                     NLREHEDELLIDLGTNGEIVVSSGGSLLCCSTAAGPAFEGGNIACGVGNVSGAIEKVS
                     FDGSLTLKTIGGTPPSGICGSGLISIVSLLLKNGLIEASGRFSNLESWPGELRARFDK
                     KCNGFMLSKDVYITLKDLRQFQLAKAALRTGIEVLLKSFYKRRLKKIRIAGGFGSKID
                     IGDLFITGILPPGPDCEIAVEGNTSLRGAIATLLYRSSLAEINAIIGCSKYIELSSLD
                     EFRNMYIDHMGYV"
     gene            4187..7261
                     /locus_tag="PLD15_05295"
     CDS             4187..7261
                     /locus_tag="PLD15_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPB63352.1"
                     /translation="MYYTKKDAYGKFSRAIGELVPYSIVRRNLKNTWIFQNREHVNPG
                     FLWSPRDFPVHFCRKIMLPYLHESEELSLKLWFGGESLVKIDGRTYGELNEHHRELDV
                     TEFAGKRILVEVETVPKGLFGSSVESPVFSEAFLIIYDTQTKGFITKLRNILEVFMHS
                     EDEVLCEKLYGVLNRTLSLVKIPRDSTNYARGLLDDPAIAREVTSIWKAEDFALAETR
                     SLPESFRRSFVEAESFLDRELRVIKDSTTTFGEMTVAGHAHIDYAWLWPLSETRRKIR
                     RTFANAVRLSKKYGDFVYSQSSAAMYADIMESDPELFGEIKRLIFEGRWEAIGGMWVE
                     SDTNLVNGESLVRQFLYGQRFFERQFGKRSRTAWLPDVFGFTWILPQIMKKAGIDFFF
                     TTKIYWSEKNKPQRDLFRWRGIDGSEVIYHSFNNPGNGYNGLLGPKEILTTMKNYKDK
                     ALHNETIFSFGYGDGGGGPTDEMLDNYRVLRDIPGMPTLKMRPFGEYFEDLPEGLELH
                     VHDGELYLELHRGTYTSQGRMKMAHRNAECSLYLAEALGTVTGYDTGKMERLWQTLLH
                     NEFHDILPGSSIREVYEDALGELFELKNEATRMIEGSLGELSDRDRTYLSLFNPSSSP
                     RRVFFSLPQELELSLETGEKVVAQKLQNGETLYYSDTEIAPMGSIILKASGSEMVMKR
                     ILETTAAGQENTLENDLLLVRVDEEGMSIYDKEFSREVFNGKAALMIYKDIPTYWDSW
                     DIERDYHLTGERLAPSHISRMESGKIRQSLRVVFNLGMTRVIQDITLHGHSRRVDLFN
                     TIDWHMRRTMLRANLPLNVLTRVARYDLSCGYIERPTTINNDFQEAAFEVPAHRWVDL
                     SEYGYGVSVLNNGKYGHGVRESTVSLNLLRSPIFPDFFADEGRHEFVYSIYPHGGSDL
                     LGTLIEGESINRDLLTIEGRLRLERPFIEMGAPTIGIMALKLAEEGSGKVLRVVELLG
                     ARGRAHIRTAFKFKHAWLCNLLEERIEPLTTDGENVALDYEPFGIYTIMFEE"
     gene            complement(7245..7724)
                     /locus_tag="PLD15_05300"
     CDS             complement(7245..7724)
                     /locus_tag="PLD15_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63353.1"
                     /translation="MDILSLIKIDRREGYLARLAVLEREVKTLAHQIELLKKVEDQVL
                     RDSLFESAIIRASKLVRNSGFTIKSFREFVRQSCSRPFRKELYDLLDGFEREETLLVE
                     RIVKLKNRRDRVIVHMDPRFAFHRERDGENTVEIEDLESILDYLKRHISIFTFTPRT"
     assembly_gap    7751..7850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7901..9076)
                     /locus_tag="PLD15_05305"
     CDS             complement(7901..9076)
                     /locus_tag="PLD15_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63354.1"
                     /translation="MYGVILGSVTIGFVFSIVNMLLIPLLKESFTPAVSGFIVSAGML
                     LAAAAGPLIGLWSDYIGKRLPFIGGLVVLSFSGTLLLIVNNSVASAFGGIILAFCAYS
                     FLGPYSSLVADRAGASGANRGFGAVMGVVNVSGFAASLVINRLYDTGTDVTLFALSAG
                     VLLPFIFPLLSAPRREYFKRSGPVEKISLRSVVETISKPALFFFFSMQFFAWFSIGGL
                     FPFITSFLSSEASMSLSTAAAWFGASTLLSGVTSFFTAVTAKAFGEKRLLVVSISVIA
                     SIGCLYSALYEKLIRGTDAGYAGVIVFLLFSLGLGFYYSLSSATLTKLVEPHEIGIAF
                     GVNSIFMILSQAVSVAIMGGLISIWGYRGMILVCTTGFILSALSAVRISPSVTGAGL"
     gene            complement(9078..>9267)
                     /locus_tag="PLD15_05310"
     CDS             complement(9078..>9267)
                     /locus_tag="PLD15_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63355.1"
                     /translation="SIRMGASVGGDTDTIAALAGLLSALHSGDHNIPEEIVEEVLNVN
                     RLDLSEIAEMIEKRRKSV"
BASE COUNT         2333 a         2244 c         2407 g         2183 t
ORIGIN      
        1 tggggctcta tacagaaaga caagtacggt agaaggtctt tataaaaagt tgtagctgag
       61 tgccgatctt tcgataacac ggaggtgtcc ggatttgtat ggctggataa agtttctggg
      121 atggttcaat ttttcgcttt tggtactgaa ctcatcgctt ttcataacgc gatggtttta
      181 cagaaaattc aacaaaaaac ttcagaaatt cccctgggaa ggtttcaaga accttatgaa
      241 gtttctggca aggatacatc cgtacacagg aagtgtattg cttataacgg ctctttacca
      301 cggctatctt gccacgggag ggttcgtgat ctacagtggc agtctggtct tcttcggcgt
      361 tctcgcacaa ttcatagtct atcttctggg aaagtacgta ccggcgatga agaagagctg
      421 gcgacctatc caccgtttgc tcatgctcgt tacctgggct ctcttcttcg ttcatgtgtt
      481 cggtccttac tcaatctgga ttccgatctt ttgactgttc cataaaactc aagctctcga
      541 tgaaaggcag aacaacctcc aggaattcct gggggttttc ctcttgaggc acgtggccgg
      601 tgtcgggaaa aacgtgcagc accgcgtttg ggatttcttg tgatagtctt acagagtctt
      661 cgacgggcac tattctatcg tcgtccccac tgataacgag ggtcggtata tttatacttc
      721 ccagttttga aacgtcgaac ttctttctag cgagagtcag ctcccagaga gccctgtccc
      781 agttgttggc tttcaagggt ttctcgtaac cctcgagcat ctccggcgtt atttcagagg
      841 gatcatgcca ggccaagtct aggagcgatc ccgcgttgtt gagaaaagaa cgtgagacca
      901 gcggtccgag atgccttccc tgaggggtcg aggtgagcaa tttgaagaag aggttatcgc
      961 tgtcgttcgt gtaaacggcc gcatcgacca tcaccaggcc gatcaccctt tcgggatgtg
     1021 cgatcgcaat ttccagggcc gtcaggccgc cggctgaatt gcctatgagt acggcctttt
     1081 cgaaccccag atgatccatc aatcgcaccg tcagctctac ctggttttct ggagtgtaag
     1141 ggttgaaatc ctttatctct tcccctattg gtctggaagt caagccgaag ccgggtctgt
     1201 cgaaggccat taccgtgaac cggtcggcga gaggcgagaa aacttcccgc caagaaaaga
     1261 tactggcccc gaacccgtgg aggagcacaa ccggcggaaa gccttcgccc gatactttgt
     1321 agtgtatgtt cacgttctcc aaccagacaa agcggctgtc ttcatcggcc agaagcgatg
     1381 gatctaccgt acctttcaaa tctggaaccg gtatgaggaa gggcacaagt aaaatcactg
     1441 caagtactgc cacaccaatg ataagtatct tcaatttaca tactcccttt gatcacttct
     1501 ctttaatagc gttaaaggcc ttgctgacga gagtctcggg atcgactccg tctaccggca
     1561 gcagggagta gccatagctc aagccgaagg aacagccggg tatattaccc ttccatgttt
     1621 cgaaggtctc gcccattttt ctcattatct tctccgcatc ttccggtctc ttcaattctt
     1681 ccgcaagcaa gagaaactct cttctcgaaa acctgcacag tgtatcgctt ttccttatgt
     1741 tccgtgaaag atcctgggcc agatctatga tcagcagttc cggattagaa tcgtttctgg
     1801 agccgatttt gatctccatt gcggctacca tcgagaggtc cgaatgtctt ttgcccctga
     1861 atatagacat gttgaggcgg tctctgaaga gaatgtagtt gggaaggccg gtctcgccgt
     1921 cataatgggc gagctgtgcg atcgtgcttt cggcgatctt caggtcggtg atatcttcta
     1981 tctggagaag gaagtagtcc tttccgttca aagagagcgg tttaaagaag tatctgaaag
     2041 atctaccacc ctgctcctgc tggctgcact cgaatcggcc gctcaacgtc tcttctctgg
     2101 cgactagttc ttcgatcttc tcctttattt tgccttcaca cttgcttgcc agcttgattc
     2161 cggcttcgtt agcgtacaac agatccgccc taacgccgta tatcagaaat ccacagtcga
     2221 gtttgtttat tgcgtgtttt ataagctcca tatccacatt tacttcactt tccacttgat
     2281 tccccccttc cgtactctgt agtcctccta aagcttttcg tctaagttat tttacaacaa
     2341 aggggtgctt gaataatact gttcgacatc tactatcgat ttgaactgcg agcaagatga
     2401 atgtaagatt gtaagggaag agtggaggtg tactttgaga ttgattgtcc attctggaga
     2461 agatgtgttt gaagatgatt ttcctcccgg aacgcccctg ctttcggctt taggtgaact
     2521 gggaatagtt atagatacac cctgtggtgg cgcgggaaag tgtggcaagt gtagagtacg
     2581 ggtcgacctg gacagtatgc tttccccgcc gggccaggcg gaacaaacta tcctgggaga
     2641 ggctttgaaa aggggaatca gactgtcctg catggccgag atcaccggag actgccaagt
     2701 atacctcgat cccggatact tcgatgccat cgccacgatc gacgaaatct cttttgaagg
     2761 gtgcgatcct gtagtaagac ggcaaagctt cgttatccct tcaggtgatg gggtgagaaa
     2821 ccaggtatcg gcgctggtgg aggagtatct tggagaagat ctggatctgt cggattttta
     2881 caccggatct ctcgagggag aagtctcggt ctacacgaga ccgggaaagc tggtcgcgat
     2941 agacggccgg agaagcttca atctgggagt cgcggtcgat atcggcacga ccaccatagc
     3001 caccgccctg cttgatctgg acagtggtga agttctcgcg aaagaaaaca cgatgaaccc
     3061 tcaaagagct ttcggacacg atgttatctc gcggatcact cacactgtcg ttgaacggga
     3121 gggactgaaa actctttcgt cggtggtaag gaaggccgtt tcgaaactga tcgaagggct
     3181 ctgtgagcaa tcggggagac ggtccggaga tataaacgag gtggtggttt ccgggaacac
     3241 gacgatgact caccttttgt tcggatttcc ggtgagatct atagccaccg ctccgttctc
     3301 gccattttac ggaggaatcg tgaggaagaa ggccggggaa atcggcctgg agctgagccc
     3361 tttttccgag gttctggccc ttccggcgat ttcgggctat gtgggaggag acatagttgc
     3421 agggatccta tccaccaatt tgagagaaca tgaagacgag ctattgatag accttggcac
     3481 caacggcgag atagtcgtct cttcgggagg aagcttactg tgctgctcca cggccgccgg
     3541 ccccgctttt gaaggcggta acatagcgtg tggagtcggc aacgtctccg gggcaatcga
     3601 gaaggtctcc ttcgacggaa gcctgacctt gaaaactata ggaggaactc caccctcggg
     3661 catttgcgga agtggattga tttctatcgt ttccctgctt cttaagaacg gccttataga
     3721 agcgtcggga agattttcga accttgaatc ctggcccggg gagctgcggg ctagattcga
     3781 taagaaatgc aacggattca tgctttccaa agatgtttac ataaccctga aggacctgag
     3841 acagttccag ctggccaagg ccgctttgag aactgggatt gaagttcttt tgaagagctt
     3901 ctacaagaga agattgaaaa aaataaggat agccggcggt tttggaagca agatagatat
     3961 tggagatctc ttcatcaccg ggatccttcc tccggggccg gactgcgaaa tagccgtgga
     4021 gggaaacacc tcgctgaggg gcgctatcgc gactcttctt tacagatcca gcctggccga
     4081 aatcaatgcg ataattggct gttcgaagta tatagaactt tccagtctgg atgagttcag
     4141 aaatatgtac atcgaccaca tgggatacgt ttgagaaggt gagtatatgt attacacgaa
     4201 gaaagatgcg tatggaaagt tctctcgcgc aatcggcgaa ctcgttccct attcgatcgt
     4261 caggagaaac ctgaaaaaca cctggatatt ccagaaccgg gaacacgtca atccgggttt
     4321 tttgtggtcg ccgcgggact ttcccgttca cttctgcaga aaaatcatgc tgccatacct
     4381 tcatgaaagc gaggagctgt cgttgaagct ctggtttggc ggcgagagtc tggtgaaaat
     4441 agacgggagg acctacggcg agctgaacga gcaccacaga gagctcgatg tgacggaatt
     4501 tgccggaaag agaatactcg tcgaggtcga gaccgttccc aaaggtcttt ttggatcgag
     4561 cgtggagagc cccgtctttt ccgaggcctt tttgatcatc tatgatacgc aaaccaaggg
     4621 cttcataact aaactgcgca acattctcga agtcttcatg cattcggaag atgaagtttt
     4681 gtgcgaaaaa ttgtacggcg ttcttaacag aactctctct ctggtcaaaa ttcccagaga
     4741 ttcgacaaac tacgcgcgcg ggctgctcga cgatccggcc attgcgcgtg aggtaacttc
     4801 tatctggaag gcggaggatt tcgccctggc cgaaacgcgt tcgctcccgg agtctttcag
     4861 aaggagtttc gttgaagcag agagttttct tgacagagaa ctgagggtta tcaaggactc
     4921 cactacaact ttcggagaga tgaccgtggc cggtcatgct cacatagatt acgcgtggct
     4981 atggcctctg agcgagacac gcagaaagat caggagaact ttcgccaacg ccgtcaggct
     5041 ttcgaaaaag tacggcgatt tcgtctattc ccagtcttcg gccgcgatgt acgcggatat
     5101 aatggagtcc gaccctgaac tgttcggaga gataaagaga ttgatctttg aaggccgctg
     5161 ggaagcaatc gggggtatgt gggtcgagag cgacaccaat cttgtgaacg gtgaatcgct
     5221 cgtgaggcag tttttgtacg gccagcgctt cttcgagagg cagtttggaa agaggagtcg
     5281 cacggcttgg cttcccgatg ttttcggctt cacctggata ctgccccaga taatgaagaa
     5341 ggccggtatc gacttcttct ttacgacaaa gatctactgg agcgagaaga acaaaccgca
     5401 aagagatctc ttcaggtgga gaggcataga cggttctgag gtgatctatc acagtttcaa
     5461 caaccccgga aacggataca acgggcttct tggaccgaag gagattctca caaccatgaa
     5521 aaactacaaa gacaaagccc tacacaacga gaccatcttc tctttcggtt acggcgacgg
     5581 cgggggcgga cctaccgatg aaatgctcga caactacagg gttttgaggg atattcccgg
     5641 gatgcccacg ctcaagatga gacccttcgg ggagtacttc gaagatctgc cggagggtct
     5701 ggagcttcac gttcacgacg gagaattata ccttgagctt cacaggggga cctacacgtc
     5761 ccagggaaga atgaagatgg cccacaggaa cgccgaatgt tcgctatatc tggccgaagc
     5821 gctcggcacc gtgaccggct acgataccgg aaaaatggaa agactctggc aaacgctcct
     5881 tcacaacgaa ttccacgaca tacttccggg ttcttcaata agagaagttt atgaagatgc
     5941 gctgggtgag ctattcgaac tcaaaaacga ggcgacgcga atgatcgaag gctccctggg
     6001 tgaactctcc gatagagaca ggacctatct atcactgttc aacccctctt cctctcccag
     6061 aagagtcttc ttctctcttc cgcaagaact cgaactgtct ctcgagacgg gcgagaaggt
     6121 tgtggcccag aagttgcaga acggggaaac gctttattat tccgataccg agatagcacc
     6181 gatgggttcg ataattctga aagcttccgg cagtgaaatg gtcatgaaga gaattttgga
     6241 gacgaccgca gccgggcagg aaaacacgct tgaaaacgat cttctactcg tgagggtaga
     6301 tgaagaggga atgagcatct acgacaaaga gttctccaga gaggttttca acggaaaggc
     6361 cgcactgatg atctacaagg atattccaac ttactgggat agttgggaca tcgaaaggga
     6421 ctaccatctg accggtgaac ggttggcgcc ctcgcacata agcaggatgg aatcgggaaa
     6481 gatcaggcag agcttgaggg tggtcttcaa cctgggaatg accagggtga ttcaggatat
     6541 aacccttcat ggtcattcaa ggagagtgga tctcttcaac acgatcgact ggcacatgag
     6601 aagaacgatg ctcagggcca acctgccttt gaatgtactt acacgtgtag cgagatacga
     6661 tctctcgtgc ggatacatcg aaaggccgac gaccatcaac aacgattttc aggaggctgc
     6721 tttcgaagtt cccgctcaca gatgggtcga tctttccgag tacggttacg gagtatcggt
     6781 tctcaacaac ggaaagtacg gtcacggagt gagggaaagc accgtctcgc tcaatctgtt
     6841 gcgttctccg atcttccccg atttctttgc cgatgaaggc agacatgaat tcgtctattc
     6901 catatatccg cacggcggga gcgatctgct gggaaccctc atagagggcg agagcatcaa
     6961 cagagacctc ctgaccatcg aaggaagact caggctcgaa agaccgttca tcgaaatggg
     7021 tgcgccgacc atcggcatta tggccctgaa actggcagaa gagggaagcg ggaaggtcct
     7081 gagggtggtc gaacttctgg gcgccagagg aagggctcac ataagaaccg ctttcaagtt
     7141 caaacatgcc tggctctgca acctgctcga ggagaggatc gaacctctca cgaccgacgg
     7201 ggaaaacgtc gccctagatt atgaaccgtt cgggatatac accattatgt tcgaggagtg
     7261 aaggtgaaga tagatatgtg tctcttcaag tagtcgagga ttgactccag atcctcgatt
     7321 tcgacggtgt tttcgccgtc cctttcgcga tgaaaggcga accttggatc catatggacg
     7381 atgactctat cgcggcgatt cttgagtttt acgattcttt cgacgaggag agtttcctct
     7441 ctttcgaagc cgtcgagaag atcgtaaagc tcttttctga aaggtctgga gcagctctgg
     7501 cgaacgaatt cccggaagct tttgatcgtg aatccgctgt ttctcacaag cttggaagct
     7561 cttattatag cgctctcaaa gagagaatcc ctcaaaacct gatcctcgac ctttttcaag
     7621 agctctatct ggtgcgcgag agtcttcact tccctctcca gtacggctaa cctcgccaga
     7681 taaccttctc tcctgtcgat tttgattaaa gacagtatat ccatatacag attatatatt
     7741 cacctgcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccatatacag
     7861 attatatatt cacctgcggc cagccggaag agatggaggt tcataacccg gctccggtaa
     7921 cggacggtga gatcctgact gcagacaggg ccgaaagtat aaagcccgtc gtacagacca
     7981 gtatcatccc tcggtatccc cagatggata tcaagcctcc catgatagcg acggaaacag
     8041 cctgtgaaag tatcatgaaa atactgttga cgccgaacgc gatccctatt tcgtgcggct
     8101 ctacgagttt tgtcagggtc gccgaactca acgagtaata gaagccaagg ccgagcgaga
     8161 agagaagaaa gacgattaca ccggcataac cggcgtcagt tccccttatc aatttctcgt
     8221 aaagggccga gtagaggcaa ccgatcgaag cgataaccga tattgaaact accagcaaac
     8281 gtttctctcc aaaagccttt gcagtaacgg ccgtgaaaaa ggaagtaact cccgataaaa
     8341 gcgtcgaagc gccgaaccat gcagcggccg ttgagagaga catagaagcc tctgaagaga
     8401 ggaaggatgt tatgaaaggg aagagaccgc cgatggaaaa ccacgcgaag aactgcatag
     8461 aaaagaagaa aaagagcgcc ggtttcgaaa tggtttcgac aaccgatctc agacttatct
     8521 tttcgacagg accggaccgt ttgaaatact cgcggcgtgg cgcagaaagc aaaggaaaga
     8581 tgaagggcaa caatacaccg gcagaaaggg cgaatagagt tacgtcggtg cccgtatcgt
     8641 ataatcggtt tatcacgagc gaggccgcaa aacccgaaac gttcaccacg cccatcaccg
     8701 ctccgaagcc acggttagcc ccggaggctc cggcccggtc ggccaccagc gatgaatacg
     8761 gccccagaaa actgtacgcg caaaaggcca gtatgattcc tccaaaggct gaagcgacgg
     8821 agttgttgac tatcagcaga agggtgccgg aaaaagagag cacgaccagc ccgcctatga
     8881 aggggagcct ctttccgatg tagtcgctcc acaaccctat caacgggccg gcagcggccg
     8941 caagcaacat gcccgcagag actatgaagc ctgaaacggc cggcgtaaag ctctctttta
     9001 gcaatggtat gaggagcata ttgactatgc tgaatacaaa tcctatcgtc acgcttccca
     9061 gtatcacgcc gtacatctca cacgctcttc cttctctttt cgatcatctc cgcgatttct
     9121 gagagatcca gtcgattgac gttgagcacc tcttcgacta tttcctcagg tatgttatga
     9181 tcccccgagt gaagcgccga caaaagacct gcgagggccg cgatcgtatc ggtgtcgccg
     9241 cccaccgaag cgcccattct tattgaa
//
LOCUS       DAOOLY010000076         9252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8712_length_9252_cov_4.153420, whole genome shotgun sequence.
ACCESSION   DAOOLY010000076 DAOOLY010000000
VERSION     DAOOLY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8712_length_9252_cov_4.153420"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLD15_05315"
     CDS             complement(<1..259)
                     /locus_tag="PLD15_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPB63356.1"
                     /translation="MYRCKIIRILESGFFDETSKSIEICEKMLKEHGYHFAGFFDVQP
                     VMSDLKECIYAASRSNDMVIVLGGTGLYREDIGPEAVLSLVD"
     gene            complement(275..2827)
                     /gene="pulA"
                     /locus_tag="PLD15_05320"
     CDS             complement(275..2827)
                     /gene="pulA"
                     /locus_tag="PLD15_05320"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HPB63357.1"
                     /translation="MRRVLFFLAILLGTTIMAFTGESMGKTAADYGAETVLIIHYHRY
                     SGNYDGWNLWVWPNMPASLDGKSYVFDRSDEFGVVSTVKFSTKHNRLGYIVRLNDWQA
                     KDVNADRFVDIPESGVAEIWVIEGEYDHVTDPATIDLRPRIKVAFLDSLNEIYASLSA
                     PVDTKMAEAKVLVKNEQRSVKSIEKADPTDVSLTNYVKVTLAGDIQPGEVSDPIELVI
                     EGFLPDEVIVRRALDDPSFYYEGQLGAVYAGNSTTFRVWSPVASYATLLLYDDYNSQK
                     STQIAMTKNSQGVWEATVEGDLHLKSYRYSLFSYGKLRETVDIYSKAVTRNSSRSVVT
                     DPARTVFDEWEMDARPLMEKFEDAIIYEIHISDMTADTKTNVVNRGKYLGLTERDRTG
                     PEGVKAGLDHLIELGVTHVHILPFNDIHYINEGVDGQYGWGYDPYLYMVPEGHYSTDP
                     ANPLSRIVEAKMMIKALHDSGIRVILDTVYNHTASTGEDSPFDQTVPYYYYRTDRTGA
                     YTNGSGVGNEIATERPMMRKHIIDSLKMWVNDYHIDGFRFDLLGLFDRETVLSIDREL
                     HELDPTLLLYGEPWGGWGANVTFTKGDQKGTQVAVFNDNLRDAIRGSVFDPKVKGFSL
                     GSRAKERRIMRGISGSIEYSYDIRDFTDDPAETINYVSAHDNQTLWDKNSAAMTDAPL
                     ELLLNAQKLSNAIVLLSQGIPFLHGGVDFARTKNGNDNSYNAGVELNKMDYSRKAQFI
                     DLFNYYRGMIELRKSHPAFRMATAQEIRENLVFLDTPRNIVAFIINGEAAGDTWKEIL
                     VIFNGNIEDAKITLPNGSWNLAMDEARVSNESLGEVSGEVILRAASAYVLYK"
     gene            complement(2841..3797)
                     /locus_tag="PLD15_05325"
     CDS             complement(2841..3797)
                     /locus_tag="PLD15_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63358.1"
                     /translation="MRSIVFNPLGLVLAYLLLLPVLLFGNAGVVSSLFGELEYVTPEA
                     SPNGHEVLIVGSNHGSSLSILEGGSLYTVTLLGVDFPKLNPYDGINLSLNSIMRSIVG
                     LKAIVESSKKFNFDGPAYLWLKDGERTVLLQSLILSNGLGNFNGQGYINLYDAQQHAK
                     SKGLGIWEENRDSLTKNSVSNLPSAQTTTTAGHMSSWVELIDVRIFDNGSFGLITLVT
                     REAGSMAGTIVSVVSNTDITKDEDSFTINDQRELKAGESYVVLLTIDRAQTPSFNYNT
                     IWVTYLDPSGGFLGLKNLNGARLLYEYEYSRKINGYVVTPLN"
     gene            complement(3937..4293)
                     /locus_tag="PLD15_05330"
     CDS             complement(3937..4293)
                     /locus_tag="PLD15_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic resistance protein VanZ"
                     /protein_id="HPB63359.1"
                     /translation="MRKLIVPLTTALYLSVIIYFYLAPARSGLIIGSDKFMHFSGFFA
                     GGLWLSLIHLLKTHRVNLLVVSIFLITGPVVLEMLQKFSPGRSVDPLDILANYLGWLV
                     PVSLYAFIKLIRSVIL"
     gene            4385..4576
                     /gene="rpmB"
                     /locus_tag="PLD15_05335"
     CDS             4385..4576
                     /gene="rpmB"
                     /locus_tag="PLD15_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPB63360.1"
                     /translation="MSKVCEICGKSPTTGNMVSHSNKKAKRWWKPNVHNVRAMVNGEV
                     KRVKICSKCLKAGKIQRAV"
     gene            4625..5749
                     /gene="alr"
                     /locus_tag="PLD15_05340"
     CDS             4625..5749
                     /gene="alr"
                     /locus_tag="PLD15_05340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB63361.1"
                     /translation="MNSRKTFASIDLEAYRYNLRYLTSKASPAKVMAVVKADGYGHGA
                     LQLARIAQDEGLGYLAVAFLEEGLKLREAGIKIPILVLNYVEPSSFEAAFENKLTLTI
                     VSHEQLKAIEASMKSRAAINSAFHIAVDTGMRRLGLEWRESVNLLKASLDLGLNVEGA
                     YTHFATADDKSSDLVAEQMKAFLSFLSQAERITKKTLLKHISNSAGILYVDNSRFDYV
                     RAGIASYGLQPSSQRDENLRPVLQWKTCVSFVKKIYPGYGVSYGQTFVADREMTVATI
                     PVGYADGYNRHLSNRGCVIISGRRCPVLGRVCMDQFVVDVTHLDYSPNVGDEVVLIGN
                     QGDENITAEEMAEWNGTINYEVTCAISSRVPRLYMKGENI"
     gene            5746..6798
                     /locus_tag="PLD15_05345"
     CDS             5746..6798
                     /locus_tag="PLD15_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB63362.1"
                     /translation="MKLRDIIGNDVLNQINPDHDKANKDGYPFVSDLRPGSTLLSVFK
                     VHSKRIQEARDGKKFLLLTLSDKTGAIRAIDWFNAEVNDSRLDQGTAVRVSGKLVLYE
                     DRIQLNVDPDGIQILEAGQYDPERFLAITSKDIPSMYDELLGTINGIVNENIKALLLE
                     IFQNDRKFIEKFIVSPAAAKVHHAYKGGLLEHTMSVVELCQFFARKYNDSLDGELLVA
                     GALLHDIGKVYEYAITQTGIDRTNDGELVGHIAMGIEIINKAIPKLTGFPRYLQTEIK
                     HLLLSHHGEMEWGSPVVPKTTEAIVLHMADDLDSKVAQFREIEEREFNGASASWSNYD
                     RFLNRRVFMRNRKSGD"
     gene            6821..9037
                     /gene="topA"
                     /locus_tag="PLD15_05350"
     CDS             6821..9037
                     /gene="topA"
                     /locus_tag="PLD15_05350"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB63363.1"
                     /translation="MPDKLVIVESPAKAKTIERYLGKGYEVTASKGHIRDLPESNLGI
                     DTETFEPTYEILKGKEKIVAELTKKAQGKRVLLASDLDREGEAIAWHIAQLLGLSSKD
                     TNRIVFNEITESAIKEAVGKPRTIDMSKVEAQVARRILDRIVGYKLSPLLWRTMTRGL
                     SAGRVQSVALKFMVELEKKIMVFVPHDFFKIFMLVGEDRFTLTKLHGKKFGNRSITTQ
                     EELDTVLKELKAGLFFVEEVKKRLSKRASPMPFITSTLQQSAISELGWSASRTMKVAQ
                     QLYEGIETEKGQIAFITYMRTDSTRISSQAREKAVEIIKNNYGERYIGPVKASKGGKK
                     VQDAHEAIRPTYPETCPEVAKKLISGDFLKLYTLIWNRFLASQMSGAEYDVTEVNVVD
                     KGDRFTFTLVGEKRVFDGFEKVLTRSSKDELNHEYNQGQEIRPDNFVWEKETTKPPSR
                     FTEASLVKELEKRGIGRPSTYATIISTLLERKYVLHQAKELRPTLLGAIVNEFLTDYF
                     PDVVDTNFTANMENELDEVENGSKKWKEVIESFYSEFKTDLDHIDRKIKNGELKIEFP
                     TDRECECGSHFKVVFGRYGGYLKCPECDKNESIDITVFSGVIDGKVILKNILQEQKKE
                     REIDEKCPQCGSSLLLRKGRFGEFIACSAYPKCKYTRNVEIPAPCPKCGGTVGKLRSK
                     KGKNYFKCSSCGELYWSEPSDRKCEVCDSTLFIRVKRGGKKVHYCEKCKKEYSVEE"
     gene            9044..>9252
                     /locus_tag="PLD15_05355"
     CDS             9044..>9252
                     /locus_tag="PLD15_05355"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730266.1"
                     /note="D-alanine--D-alanine ligase; DdlA; DdlB;
                     cytoplasmic; catalyzes the formation of D-alanyl-D-alanine
                     from two D-alanines in peptidoglycan synthesis; there are
                     two forms of this enzyme in Escherichia coli; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPB63364.1"
                     /translation="MERERIAVVYGGLSHEREISIKSGYNVIASLEKRGSEVVPFDLK
                     RSNIEKLLNIDADLFFLALHGKFGE"
BASE COUNT         2534 a         2175 c         2256 g         2287 t
ORIGIN      
        1 tatctaccag agagagaacc gcctcgggac caatatcctc tctgtaaagg ccggtccccc
       61 cgagtacaat taccatgtcg tttgacctgg aagcggcata aatgcactct tttagatcac
      121 tcattactgg ctggacatca aaaaaaccgg caaaatggta accgtgttct ttgagcatct
      181 tttcgcagat ttcgatcgat ttggatgttt cgtcgaaaaa ccccgattct aggatcctta
      241 tgatcttaca cctgtacatc tctacctccg gatatcattt gtaaagcaca taagccgaag
      301 cggcccttag aatgacttca ccactgactt ctcccagcga ttcattggat accctcgctt
      361 catccatggc caggttccag gaaccgttgg gaagtgttat cttcgcatct tcgatgtttc
      421 cgttgaatat gaccagtatc tccttccatg tatcgccggc cgcttcaccg ttaataatga
      481 aagcaacgat attcctcggt gtgtctagga agacaaggtt ctctcttatc tcctgagcag
      541 tagccattct gaaggccggg tgagacttcc gcaactctat cattcctctg tagtagttga
      601 agagatctat aaactgggcc ttcctggaat agtccatctt gttgagctca acaccggcgt
      661 tatagctgtt gtcgttgccg ttcttggttc tggcgaaatc gacccctccg tgaaggaagg
      721 gaataccctg cgagagcaac acgatcgcat tcgaaagttt ctgtgcgtta agtagcagct
      781 ctagcggtgc atcggtcatc gccgcgctat ttttatccca gagagtctga ttgtcgtgcg
      841 ccgaaacgta gttgattgtt tctgcagggt catcggtgaa gtcgcgtata tcatagctgt
      901 actctatgct accgctgatg ccgcgcatga tcctgcgttc cttggctctg cttccaagtg
      961 aaaaaccctt tacctttgga tcgaagacgc ttccgcggat cgcatctctc aagttgtcgt
     1021 tgaagacggc gacctgtgtg cccttctggt cgcccttggt gaaggtcaca ttggctcccc
     1081 aaccgcccca gggttcgccg tagagcagta gagtgggatc caattcatga agctctctat
     1141 cgatggaaag tacggtttcc ctatcgaaga ggccgagcag gtcgaatctg aaaccgtcga
     1201 tatggtaatc gttcacccac atctttaggg agtctataat gtgcttcctc atcatgggcc
     1261 tctccgtagc tatttcattt cctacgccgc taccgttggt ataagccccc gtcctgtcgg
     1321 tcctgtaata gtaataagga acggtttgat cgaagggcga gtcttcgcct gtagaagcag
     1381 tgtgattgta aactgtatcg agaataacgc gaataccact gtcgtgaaga gcctttatca
     1441 tcattttcgc ttctactatt ctgctgagtg gattggcagg atcggtactg taatgacctt
     1501 cgggaaccat gtataaatat gggtcgtacc cccagccata ctgcccatcg acaccctcgt
     1561 tgatgtaatg aatatcgttg aaagggagga tgtgaacgtg agttacacct agctcgatca
     1621 gatgatccag gcctgctttc accccttcgg gaccggttct atccctttcg gtaagaccga
     1681 gatatttgcc ccgattgact acgttcgtct ttgtatcggc cgtcatatcg cttatatgaa
     1741 tctcgtagat tatggcatcc tcgaactttt ccatcaatgg tctggcatcc atctcccatt
     1801 catcgaaaac cgtccttgcc ggatcggtca caaccgacct cgagctgtta cgcgttacgg
     1861 cttttgaata tatatccaca gtttcacgaa gcttaccgta agaaaagagc gagtatctgt
     1921 acgatttgag gtgaagatcg ccttcgaccg tcgcttccca tactccctgt gaattcttcg
     1981 tcatagctat ctgcgtgctt ttttgagaat tataatcatc gtacaacaac aaagtggcat
     2041 aagacgcgac cggtgaccag actctgaaag tcgtggagtt gcctgcgtat actgctccga
     2101 gctgtccttc gtagtaaaaa gaagggtcgt ccagtgctct tctcacgatc acttcatcgg
     2161 ggagaaagcc ttcaatcacc agttctatag gatcgctgac ttcgcccggc tggatgtccc
     2221 cggcgagtgt taccttcacg taatttgtaa gcgagacgtc tgtgggatct gccttctcga
     2281 ttgattttac cgaccgttgt tcgtttttaa ccagaacctt cgcttcagcc attttagtgt
     2341 ctaccggagc cgacagtgaa gcgtatatct cgttcaaact gtcaaggaag gctaccttta
     2401 ttctgggcct gagatctatg gtcgccgggt cggttacatg gtcgtactcg ccctcgatta
     2461 cccagatttc ggccactccc gattcgggaa tatccacaaa cctatctgca tttacatcct
     2521 tagcctgcca gtcattcaac ctgactatgt agccgagcct gttatgttta gtggaaaatt
     2581 tgacagtcga gacgacccca aattcgtcac tcctgtcgaa aacgtaagat ttgccatcca
     2641 agctggctgg catatttggc cagacccaca gattccatcc atcgtagttg ccgctgtatc
     2701 tgtgataatg tattataagt accgtttctg caccgtaatc ggctgctgtt tttcccatac
     2761 tttctcctgt aaaagccatt attgtagtac caagaagaat cgccaaaaag aacaacactc
     2821 ttctcattat aatgcctccc tcaattaagt ggggttacaa catagccatt tatctttctg
     2881 ctgtattcat attcgtagag aagtctggct ccgttaaggt ttttcagccc caggaacccg
     2941 ccgctcggat cgagataggt cacccatatc gtgttgtagt tgaaggaagg agtctgtgct
     3001 ctgtcaattg tcagtagcac tacataactc tcaccagctt tgagttctct ctggtcgttt
     3061 attgtgaaag aatcttcatc tttcgtaata tccgtgttcg agactaccga gacaatcgtt
     3121 ccggccatgc tgccagcttc tctggtaacc agcgttatta gcccgaatga accattgtcg
     3181 aagatacgca cgtctatcaa ctccacccag ctcgacatat gtcccgctgt tgttgtggtt
     3241 tgcgcactcg gcaaattgga gacagaattt ttggtgagac tgtctctgtt ctcctcccat
     3301 atccccaatc ctttgctctt ggcgtgttgt tgggcatcat aaagattgat atagccctgt
     3361 ccgttgaagt ttcccagacc gttggacagt atcagcgact gaagaagtac cgttctctct
     3421 ccatccttaa gccacagata ggccggccca tcgaagttga atttcttgct gctttctaca
     3481 attgccttta gaccaacgat gctgcgcatt atgctgttga gggagaggtt tatcccgtca
     3541 taagggttca actttgggaa atcaacgccc agtaatgtaa ctgtatagag ggagcctcct
     3601 tcaagtatcg atagcgagga accgtgattt gaaccgacga ttagaacttc atggccgttt
     3661 ggagaggctt cgggtgtgac gtactccagc tctccaaaaa gcgaggaaac tactccggcg
     3721 ttaccaaaaa gcaaaaccgg cagtaagaga agatacgcca ggactaaacc aagaggattg
     3781 aaaacaatgc ttctcaaaaa aactccaccc cccacaaggt ctcggctcga ctgaggaaag
     3841 tataacatcc ggaatactcc gttggtagaa aaatacaaac tgctctccac tactaggaac
     3901 tctctccatc gtttgttcta ccgcctttat ttaagactat aaaataactg agcgtatcag
     3961 ttttatgaaa gcataaagac tcaccggtac cagccagccc agatagttgg ctaaaatatc
     4021 cagcggatcc accgatcggc ctggagagaa cttctggagc atttcgagca caaccggacc
     4081 ggttattaga aagatagaaa ctaccaggag attcaccctg tgtgttttga gcaaatggat
     4141 caaagatagc cagagacctc cggcgaagaa gcccgaaaag tgcataaact tatcactgcc
     4201 gattatgaga cccgatctgg ccggtgcaag ataaaagtat attatcactg atagatacag
     4261 tgccgttgta agtggtacaa tcaatttcct catcatggaa ttatataccc cgcaacggtg
     4321 agttgaaaag ctgatacatc tttaatataa tgaagtgtaa tgcaattcga gaggaggaca
     4381 atacatgtcc aaggtttgcg agatctgtgg gaaaagcccc actacaggaa acatggtttc
     4441 ccactcgaac aagaaggcca agagatggtg gaagcccaac gtacacaatg taagggccat
     4501 ggttaacggt gaagtaaaaa gagtgaagat ttgcagcaag tgccttaagg caggaaagat
     4561 acagagagca gtatagtgca gagcgcgcca tgcgcgcttt tcttttagaa tatgggaagg
     4621 cattatgaat agcaggaaaa ccttcgcctc aatagatctg gaagcttaca ggtacaacct
     4681 acgatatctg acgtcgaaag cttcaccagc aaaggtcatg gcggttgtaa aggccgatgg
     4741 ttacggccat ggtgccttgc aactggctag aattgcccag gatgaaggat tgggatatct
     4801 tgccgttgcc ttcctcgagg aaggtctcaa gcttagggag gccgggatta agattcctat
     4861 ccttgtgtta aattacgttg aacccagctc ttttgaagcg gctttcgaga ataaactcac
     4921 gctcacgatc gtatctcacg agcaactgaa ggctatcgaa gcgtctatga aatccagagc
     4981 tgctataaac tcagcctttc acatcgctgt cgataccggg atgagaaggc tcggtcttga
     5041 gtggagagag tcggtgaacc tcttgaaagc gtcccttgat ctgggactga atgtagaagg
     5101 agcctatacg catttcgcca cggccgacga taagagcagc gacttagtcg ccgaacaaat
     5161 gaaggccttt ctgtcgtttc tcagccaggc cgagaggata acaaaaaaga ctctcttgaa
     5221 acatatctcc aacagcgccg gtatccttta cgttgacaac agtcgtttcg attacgttag
     5281 agccgggatc gcaagttacg gtctccagcc ttcttcacag cgtgacgaaa atctcaggcc
     5341 ggtgctccag tggaagacat gtgtttcttt tgtgaagaag atctacccag gctacggtgt
     5401 gagctacggt cagacttttg ttgcagatcg tgaaatgacg gtggctacca ttccagtcgg
     5461 ctacgccgac ggttacaaca gacatctctc caatagagga tgtgtcatta tctctggaag
     5521 gagatgtccg gtgcttggta gagtctgcat ggaccagttc gtggttgatg tcacgcacct
     5581 ggactactcg cccaacgtgg gagatgaggt tgtattaatt ggaaatcagg gcgatgagaa
     5641 tattacagca gaggaaatgg cagagtggaa tggtaccatt aattatgaag taacctgtgc
     5701 tatttcgtcg agagtgccca ggctctacat gaaaggagaa aatatatgaa actccgcgat
     5761 attatcggaa acgacgtact caatcagata aaccccgatc acgacaaagc caacaaagat
     5821 ggctaccctt tcgtctcgga tctgagacct ggcagtactc ttctgtctgt tttcaaagtg
     5881 cactccaaga gaatccagga agcccgtgat ggaaaaaaat tcctgctcct cactctatcc
     5941 gacaaaacag gtgccataag ggcgatagac tggttcaacg ccgaagtcaa cgattccaga
     6001 ctcgaccagg gcactgccgt gagggtgtcg ggtaaactgg tactttacga agacagaatc
     6061 cagctcaacg tcgatcctga cggcatacag atactcgagg ctgggcagta cgaccctgag
     6121 aggtttctgg ccataacatc caaagatatt cctagcatgt acgacgagtt attgggaact
     6181 ataaatggaa tcgtcaacga gaacataaag gcactgctac ttgagatttt ccagaacgac
     6241 aggaagttca tagagaagtt catagtttct cctgcagcgg cgaaggttca ccacgcatac
     6301 aaaggggggc tccttgagca taccatgtct gttgttgagc tctgccagtt tttcgcgcgg
     6361 aagtacaacg attcactaga cggtgaactc ctggtggcgg gtgcgttgct acacgacata
     6421 ggcaaggttt acgaatacgc gatcacccag acgggaatag atagaaccaa cgatggcgaa
     6481 ctggttggac atatcgccat gggtatcgag ataatcaaca aggctattcc caagctaaca
     6541 ggctttccta gatatcttca aaccgagatc aagcacctgt tgctttcaca ccacggggag
     6601 atggagtggg gctccccagt cgttcccaag actaccgagg cgatagtgct tcacatggcc
     6661 gacgatctcg attcgaaagt tgcgcagttc agggagatag aggaacgaga attcaatgga
     6721 gcctcggcct cctggagcaa ttacgatcgc ttcctcaaca gaagggtctt catgaggaac
     6781 aggaaatccg gcgattgaaa ttcagaaatg gagtgatatc ttgccagaca aattagttat
     6841 cgtagagtcc ccggcaaaag cgaagaccat cgagaggtac ctggggaaag gctacgaagt
     6901 tacggcttcg aagggacata taagagatct cccggaatcc aatctcggga tagatacgga
     6961 gaccttcgag ccgacttatg aaattctcaa ggggaaggaa aagatagtcg cagaacttac
     7021 aaagaaggcg cagggcaaga gggttcttct cgcgtccgac ttggaccgtg aaggcgaggc
     7081 gatagcctgg cacatcgcgc aacttctggg gctatcaagc aaggatacaa acaggatcgt
     7141 cttcaacgaa ataacggaat ctgccataaa agaggccgtt ggcaaacccc ggacgataga
     7201 catgtccaaa gtcgaagcgc aggttgcccg ccgtattctc gatagaatag tcggctacaa
     7261 actcagccct cttctgtgga gaacgatgac cagaggattg agcgccggaa gagtacaatc
     7321 ggtagctttg aagttcatgg tagagttgga gaagaagatc atggtcttcg tgccccacga
     7381 tttcttcaag atcttcatgc ttgtgggcga ggacaggttc acactcacta aacttcacgg
     7441 gaaaaagttt ggaaacagat cgatcactac gcaagaggaa ctggacactg tcttgaaaga
     7501 gctaaaggcc ggcctttttt ttgttgagga agttaaaaaa aggctttcaa agagggcttc
     7561 tcccatgcca tttataactt ctacgcttca gcagagcgcc ataagcgaac tcggctggag
     7621 cgccagccgg acgatgaagg tcgctcagca gctgtacgaa gggattgaaa cagagaaggg
     7681 gcagatagct ttcataactt acatgagaac cgactcgacc aggatttctt cgcaggccag
     7741 agagaaggcc gtcgagataa tcaagaacaa ctacggtgaa agatacatcg gtcctgtaaa
     7801 ggcatctaaa ggtggcaaaa aggtccagga cgcccatgaa gcgataaggc caacttaccc
     7861 ggagacctgt ccggaagttg cgaagaagct gatttcggga gattttctca aactttatac
     7921 cttgatatgg aacagatttc tggcctcgca gatgtccggt gcagagtacg atgtgacaga
     7981 agtcaacgtg gtagataaag gtgatcgttt cacctttacc ctcgtcggcg agaaaagagt
     8041 cttcgatggt ttcgaaaagg ttttgaccag atccagcaaa gatgaactca atcatgagta
     8101 caaccagggg caggagataa ggccagataa tttcgtctgg gaaaaggaaa ctaccaagcc
     8161 cccctctaga tttaccgagg cttcgctagt caaagaactg gagaagaggg gtatcggccg
     8221 gccctcaact tacgccacga taatctcgac cctgctcgaa agaaagtatg tactccatca
     8281 ggccaaagag ctgcgaccga cactgctggg agctatagtc aacgaattcc tcaccgatta
     8341 ctttcccgac gtggtcgata caaacttcac ggcgaatatg gaaaatgaac tggacgaggt
     8401 tgaaaacggg tcaaaaaagt ggaaggaagt aatagagagc ttctacagcg aatttaagac
     8461 cgatctggac catatagaca gaaagataaa gaacggagaa cttaagatcg agtttccaac
     8521 agatagagag tgcgaatgcg gtagccattt caaagtggtg ttcggcaggt atggcggtta
     8581 cctgaaatgt cccgaatgcg ataagaacga atcgatcgac attacagtct tttcaggcgt
     8641 tattgatggg aaggttatcc tgaaaaatat tctgcaagag cagaaaaaag aaagggagat
     8701 agatgagaaa tgtcctcaat gcggttcctc gctcctgctt agaaagggta ggtttggcga
     8761 attcatcgcc tgctcggcct atcccaaatg caagtacacc aggaatgtgg aaattccggc
     8821 cccatgtcct aaatgcggag gaacagtcgg aaaactgcgc agtaaaaaag gcaagaatta
     8881 cttcaaatgc tcttcctgcg gcgagcttta ctggagcgaa ccgagcgaca gaaagtgtga
     8941 agtatgtgac tccacgcttt tcataagagt caagcgcggg ggaaagaagg ttcattactg
     9001 cgaaaagtgt aaaaaggagt actctgtaga ggagtaatcg atcatggaaa gagaaaggat
     9061 agcggttgtt tacggaggtc tctcgcacga acgagagatt tcgataaaga gcggttataa
     9121 cgtgattgct tctctcgaaa agagaggcag cgaagtcgtc cctttcgatc ttaaaagatc
     9181 gaatatcgaa aaacttctga atatagatgc ggatctcttc ttcctggcac tccacggaaa
     9241 attcggagag ga
//
LOCUS       DAOOLY010000077         9199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8773_length_9199_cov_4.328412, whole genome shotgun sequence.
ACCESSION   DAOOLY010000077 DAOOLY010000000
VERSION     DAOOLY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_9199_cov_4.328412"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /gene="rpsJ"
                     /locus_tag="PLD15_05360"
     CDS             <1..205
                     /gene="rpsJ"
                     /locus_tag="PLD15_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPB63365.1"
                     /translation="VSGPVPLPTERTLYTVLTSPHKFKDAREQFEKLVHKRLIEILDP
                     TPKTIDSLMKVDLPAGVDVEIKL"
     gene            228..860
                     /gene="rplC"
                     /locus_tag="PLD15_05365"
     CDS             228..860
                     /gene="rplC"
                     /locus_tag="PLD15_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPB63366.1"
                     /translation="MKGILGRKLGMTTLYKDGKAFGVTVVKAGPCTVVQKKTLEGGEY
                     DAIQVGFEELTPERAKKILTKPLVKKFEAAKVKPQRVLKEFKVGNINDYTVGDIIEVG
                     VFSEGEKVDVTGYSKGRGFSGAMKRWNFRGGEASHGAKFHRELGSVGNHTEPAKIWKG
                     KKMAGQYGNEKKTIKNLTVVKVDAENGLLAIYGAVPGARGGLLVIKSATR"
     gene            892..1572
                     /gene="rplD"
                     /locus_tag="PLD15_05370"
     CDS             892..1572
                     /gene="rplD"
                     /locus_tag="PLD15_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB63367.1"
                     /translation="MAQADVVNMAGQKVGTVELNEAIFNVEPNLDVMFRYVNMQLAGR
                     RAGLASVKTRSEVRGGGRKPWAQKHTGRARVGSTRSPLWRHGGVIHGPKPKDWSIKMT
                     KKMKKVALRSALSLRLKEGNLIILDDLKFDRPKTRELRNVMGSLGLDKTQKTLFILPW
                     QKDEYQNVRLSGKNIYGVKVIIADNPGNSANGNKTNIDGLNVYDIVNHEKLVITTDLV
                     RKIEEVLG"
     gene            1572..1871
                     /gene="rplW"
                     /locus_tag="PLD15_05375"
     CDS             1572..1871
                     /gene="rplW"
                     /locus_tag="PLD15_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPB63368.1"
                     /translation="MADKKYYGDVLLRPLVTEKTVAASELNKYIFEVHRDANKYTVKD
                     AVEKLFNVKVDQVNIMNMKAKPKKRGVFVGKTRSWKKAVVTLVVGYRIKELEGQH"
     gene            1888..2715
                     /gene="rplB"
                     /locus_tag="PLD15_05380"
     CDS             1888..2715
                     /gene="rplB"
                     /locus_tag="PLD15_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPB63369.1"
                     /translation="MALRKFNPVTHSRRFMLLPDYKEITRSDPEKSLVEPNRSKAGRN
                     HHGRITVRHQGSGNKRLYRIIDFKRDKLGIPARVVSVEYDPNRTARIALLQYIDGEKR
                     YMLAPKGLKVGDSILNGENAEISVGNSMPLEKIPVGTIVHNIEFLPGKGGQIARAAGT
                     YAQLMAKEGRYALLRMPSGELRRVVVKCMATIGMVGNEEHSNEIHGKAGRKRWLGVRP
                     TVRGMTMNPVDHPMGGGEGRSKGHLPQSPWGQPARGYKTRKHKKASDNLIVKRRNQN"
     gene            2735..3019
                     /gene="rpsS"
                     /locus_tag="PLD15_05385"
     CDS             2735..3019
                     /gene="rpsS"
                     /locus_tag="PLD15_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPB63370.1"
                     /translation="MSRSKKKGPFIHPSLLKKIKELNEKGEKKPIKTWSRASMILPEM
                     IGHTIAVYNGMKHIPVYISENMIGHRLGEFSPTRRFGGHADKKSKKGEVK"
     gene            3034..3468
                     /gene="rplV"
                     /locus_tag="PLD15_05390"
     CDS             3034..3468
                     /gene="rplV"
                     /locus_tag="PLD15_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPB63371.1"
                     /translation="MPQAEQRKKRSAFHKARKEAEAATPVTSAKAVARYTRISPRKAR
                     SVVNAIRNKNVGDAFQILEFSPKKASRLVYKVLRSAVANAENNFGLNVDNLYVESAVV
                     DDGPRMKRLWPRGRGRADIQQKRFSHITVVVANREETSNPQE"
     gene            3479..4108
                     /gene="rpsC"
                     /locus_tag="PLD15_05395"
     CDS             3479..4108
                     /gene="rpsC"
                     /locus_tag="PLD15_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPB63372.1"
                     /translation="MGQKVHPYGFRLGVSKDWKARWINEKNYKEYLLEDLKIRGFLKK
                     NYMAAGVSEIYIERPEPGKVVITIKCARPGVMIGKKGSEVKLLRQKLEKLITRQFQLN
                     IEEVKTPETDAILVAEDIASRIEKRASYKRAMKRAIFTAMRKGAKGIKIMVSGRLNGA
                     DIARTEWYLEGRLPLQTLRADLDYGYTTAFTKMGIIGVKVWIYKGDVQV"
     gene            4133..4558
                     /gene="rplP"
                     /locus_tag="PLD15_05400"
     CDS             4133..4558
                     /gene="rplP"
                     /locus_tag="PLD15_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPB63373.1"
                     /translation="MLMPKRVKYRKQQRGKMNGNAKGGTLVHFGEWGIKALEPHWITA
                     QQLEACRIAITRTLKRTGNLWIRVFPDKPITSKGIGVRMGKGKGDVEGWVAVVKPGKI
                     MFEIGGVPDQLAKEALEKAASKLPIRTKIVPRYQIGGEL"
     gene            4558..4758
                     /gene="rpmC"
                     /locus_tag="PLD15_05405"
     CDS             4558..4758
                     /gene="rpmC"
                     /locus_tag="PLD15_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPB63374.1"
                     /translation="MKPVELQKFTDEELLQMLEDSKRKLMDLRFQLEMNRLKNHSQIT
                     SVKRDIARIKTILRGRELGIRR"
     gene            4766..5008
                     /gene="rpsQ"
                     /locus_tag="PLD15_05410"
                     /pseudo
     CDS             4766..5008
                     /gene="rpsQ"
                     /locus_tag="PLD15_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            5071..5439
                     /gene="rplN"
                     /locus_tag="PLD15_05415"
     CDS             5071..5439
                     /gene="rplN"
                     /locus_tag="PLD15_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPB63375.1"
                     /translation="MVQLESYLKVADNSGARVIKVIQVTGGYRKRSGTIGDIVVAAVR
                     DAAPHTDFKKGDIVRAVLVRTAKEIRRPDGTYIRFDDNAAVIIDKQNQPRGTRVFGPV
                     AREIRDKGFSKIASLAQEVW"
     gene            5452..5772
                     /gene="rplX"
                     /locus_tag="PLD15_05420"
     CDS             5452..5772
                     /gene="rplX"
                     /locus_tag="PLD15_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPB63376.1"
                     /translation="MSRVKKDDLVMVISGKDRGKKGKVLKTYPSEKKVIVEGVNFTKK
                     HQRPTNQYREGGIIERESPIYVSKVMVVCPNCDKPTRIAHKILENGEKVRACKKCGEI
                     IDKV"
     gene            5789..6349
                     /gene="rplE"
                     /locus_tag="PLD15_05425"
     CDS             5789..6349
                     /gene="rplE"
                     /locus_tag="PLD15_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPB63377.1"
                     /translation="MAYEYIPLKERYENEVIPAMTKEFSYKNKLEVPKVSKVIVNMGI
                     GEGSRNADLLEIHGKELTNIVGQKPVITRAKKSIANFKLRDGMPVGLKVTLRGPRMYN
                     FLYKLINIVLPKLRDFRGVDPDSFDGRGNYALGLPEQLVFPEIVPDQVKRVQGMDIIV
                     VTTARTDEEARRLLALLGVPFKRAMV"
     gene            6361..6546
                     /locus_tag="PLD15_05430"
     CDS             6361..6546
                     /locus_tag="PLD15_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPB63378.1"
                     /translation="MAKKSIIEKWKREPKFKVRKYNRCSICGRPRSVYREFGLCRVCF
                     RQLASEGKLPGVKKASW"
     gene            6560..6964
                     /gene="rpsH"
                     /locus_tag="PLD15_05435"
     CDS             6560..6964
                     /gene="rpsH"
                     /locus_tag="PLD15_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPB63379.1"
                     /translation="MWSDPIADMLTRIRNANAAFKESVDIPASNLKKKLLDILKAEGY
                     IDDYKYIEDGKQGVLKVFLKYKGDRRNKVNVITGIVRISKPGRRIYVNRDKLPKVKSG
                     MGIAIVSTSSGVMTDKQARMKGVGGEVICYVW"
     gene            6977..7531
                     /gene="rplF"
                     /locus_tag="PLD15_05440"
     CDS             6977..7531
                     /gene="rplF"
                     /locus_tag="PLD15_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPB63380.1"
                     /translation="MSRLLKHSIKLPAGVTHSIKDNVITVKGPKGELKQEILPLVKIE
                     GDEKEIWVKTNEDLVIRKSDHKRLAKYAGTFWSLINNMVLGVTQGFVKELEIVGVGYR
                     AQLQGKKLVMNLGYAHPVEIDPPQGITFEVPAPQSVVVKGFDKYLVGQVAANIKRWRI
                     PIVYSGKGIRYKGEAIRTKVGKKV"
     gene            7549..7917
                     /gene="rplR"
                     /locus_tag="PLD15_05445"
     CDS             7549..7917
                     /gene="rplR"
                     /locus_tag="PLD15_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPB63381.1"
                     /translation="MFKHKDKKEQRQKRHLRVRAKLSGTAERPRLAVYRSEKHIYAQL
                     IDDIKGITLVSASTVDREMRDSTEKPWNVEAASQVGKLLAKKALEKGISSVVFDRGGF
                     NFHGRVKSLADGAREGGLKF"
     gene            7934..8539
                     /gene="rpsE"
                     /locus_tag="PLD15_05450"
     CDS             7934..8539
                     /gene="rpsE"
                     /locus_tag="PLD15_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPB63382.1"
                     /translation="MSEEMMNDRGSRPSREGRRNPRTSRNAPVEKQVEDNEFEERIIE
                     IRRVTKVVAGGKNLSFRVVAIVGNREGKVGLGIGSAREVPIAIRKAVLEAKKNIVEVS
                     IRNETVPHETVGKQDSARIMLKPAGPGTGIIANSPVRAVVELAGVKNILTKSLGSSNT
                     LNMARAALNGLVSLRAPQEFARLRDISLKAVFHGLGEEDGQ"
     gene            8539..8727
                     /gene="rpmD"
                     /locus_tag="PLD15_05455"
     CDS             8539..8727
                     /gene="rpmD"
                     /locus_tag="PLD15_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPB63383.1"
                     /translation="MAKSIRVRLVKSPIGYNRRQLKTVKALGLGKLDSEMVHQDTPQI
                     RGMINAVRHLLAVEELAD"
     gene            8741..9187
                     /gene="rplO"
                     /locus_tag="PLD15_05460"
     CDS             8741..9187
                     /gene="rplO"
                     /locus_tag="PLD15_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPB63384.1"
                     /translation="MAFNIDDLKPTPGSRKRSKRIGRGTGSGMGKTATRGHKGQGRAT
                     GRVSAHFEGGQTPLFRRIPVKGFKNKSAVEYATVNLSTLEERFESGSEVTPEILMKMK
                     ILKNLKDGVKILARGEITKSLNIKANAFSASAKEKIESAGGKAEVI"
BASE COUNT         2894 a         1879 c         2582 g         1844 t
ORIGIN      
        1 agtatcaggt ccggtgccat tgccaacgga gaggacgctc tacactgttc tgacatcgcc
       61 ccacaaattc aaggatgcaa gagagcagtt cgagaagctt gtacataaga gattgataga
      121 aatcctcgat ccaacgccga aaacgataga ttctcttatg aaggtggacc tccccgcggg
      181 tgttgatgtg gagatcaaat tgtaagcaga cttcggaggt gtgctgaatg aaaggtatat
      241 tgggtagaaa gctcggtatg acaactctat acaaagacgg aaaagccttc ggagttaccg
      301 tagtgaaagc cggtccatgt acagttgtgc agaagaagac cctggaaggc ggagagtacg
      361 atgccataca ggttggattt gaagaattaa ctccagagag agccaagaag atactgacca
      421 agccgctggt gaagaaattt gaagctgcca aggttaagcc acagagagtt ctcaaggagt
      481 tcaaagttgg taacatcaat gactacaccg ttggtgatat catagaagtc ggtgtctttt
      541 cagaaggcga aaaggtcgat gtgaccggtt actctaaggg tagaggtttc tctggtgcca
      601 tgaagagatg gaatttcaga ggcggggagg cttcccacgg cgcgaaattc cacagagaac
      661 tcggttcagt cggtaaccac acagaaccgg cgaaaatctg gaaaggcaag aagatggccg
      721 ggcagtacgg taacgagaag aagacaatca agaacctgac tgtagtaaaa gtcgatgcag
      781 aaaacggact cttagccatt tatggcgcag ttccaggagc gaggggcggc ctcttagtga
      841 ttaagagcgc aactagatag ccccgaaaat gtgttggaag gaggacgcaa catggctcaa
      901 gccgacgttg tgaacatggc cggacagaaa gttggtacgg ttgagctgaa tgaagcgata
      961 ttcaatgttg agcccaatct ggacgttatg ttcagatatg tcaacatgca gctggccgga
     1021 agacgtgccg gattggcatc tgtcaagacc agatcggaag tgagaggcgg gggtagaaaa
     1081 ccctgggccc agaaacacac aggaagagca agagtaggat ccaccagaag tcctctctgg
     1141 aggcacggtg gcgtaattca tggaccaaaa cccaaggatt ggtccataaa gatgaccaag
     1201 aaaatgaaaa aggtggcact gaggtcggct ctcagcctca gacttaagga aggcaatctc
     1261 attattctcg acgatcttaa gtttgacaga ccgaaaacca gagaactgag aaacgttatg
     1321 ggcagtctcg gactcgataa gactcagaaa acccttttca ttctgccctg gcagaaggac
     1381 gagtatcaga acgtgagact ttcaggaaaa aacatttatg gcgtaaaagt cataatagcc
     1441 gacaaccctg gaaactcggc caacggtaac aagaccaata tcgacggtct gaacgtttat
     1501 gacatcgtga atcatgaaaa actggtcatc accactgatc ttgtacgcaa gatcgaggag
     1561 gtgctggggt aatggctgac aagaagtact acggagatgt tctcctcaga ccgctggtaa
     1621 cggaaaaaac agttgcagcg agtgaattga acaagtacat ttttgaagta catagagatg
     1681 ccaataagta cacagtgaag gacgccgtgg agaagctttt caatgtgaaa gtggatcagg
     1741 taaacattat gaacatgaag gcgaagccga aaaaacgcgg agtctttgtt ggtaaaacga
     1801 ggtcctggaa gaaggccgtc gttactctgg tggtcggtta caggataaaa gaactcgaag
     1861 gccagcatta aggtgaggtg agatgatatg gcattgagaa agttcaatcc cgtcacccat
     1921 agcagaaggt tcatgcttct cccagactat aaagagataa cgagatctga tcccgagaag
     1981 tctctggtcg aacccaacag gagcaaggcc ggaagaaacc atcatggaag aatcacggtc
     2041 aggcatcagg gtagcggcaa caagagactg tacaggataa ttgacttcaa gagagataag
     2101 ctcggcattc ctgccagagt tgtttctgtt gagtacgacc cgaatcgaac tgccagaata
     2161 gctcttcttc agtacattga tggcgagaag agatacatgc tggctcccaa gggtctaaaa
     2221 gtcggtgaca gtatcctcaa tggcgagaac gcagagatct ctgtaggaaa ttcgatgcct
     2281 ctggagaaaa ttccggtggg cacgatagtt cacaacatcg aattccttcc tggaaagggc
     2341 ggacagattg cccgtgcggc aggtacctac gcacagctaa tggcaaaaga gggcagatac
     2401 gctctgctga gaatgccatc gggcgaactg cgaagagtcg tagtgaagtg catggcgacc
     2461 attggtatgg ttggcaatga agagcattcc aacgaaatac atggaaaggc cggaagaaag
     2521 agatggctgg gagtcagacc tacggtcagg ggtatgacga tgaacccggt cgaccacccg
     2581 atgggtggag gagaaggtcg ttccaaaggt catctaccac agagcccctg gggtcaaccg
     2641 gccagaggtt ataagacaag aaagcataaa aaggcttccg ataacctcat agtgaagcgc
     2701 aggaatcaga attaatcagg gggtgtagtt gaagatgtcg agatctaaga aaaagggacc
     2761 attcatacac ccgagcctct tgaaaaagat aaaagagctg aatgaaaaag gagagaaaaa
     2821 accaatcaaa acatggagca gagcctctat gattctcccg gagatgatcg gacacactat
     2881 cgccgtttat aacggtatga agcatatacc agtttatatc tcagagaata tgatcggaca
     2941 cagactgggc gaattctctc ccacaagacg gttcggtggt cacgcggaca agaagagcaa
     3001 gaagggtgaa gtcaaatgag gagggatgcc gatatgcctc aagcagaaca gaggaagaag
     3061 cgttctgcct ttcataaagc tagaaaagaa gcagaagcgg cgacacctgt taccagcgcg
     3121 aaagctgtgg ctagatatac caggatctct cctagaaagg caaggtctgt ggtaaatgcc
     3181 ataagaaaca aaaacgtggg agacgctttc cagatactcg agttctctcc gaagaaagca
     3241 tccagactcg tgtataaagt tctccgttcc gcagtcgcca atgcagagaa caatttcggg
     3301 ctgaacgtgg ataacctcta cgtcgaaagc gccgtagttg acgacggtcc gagaatgaag
     3361 aggctctggc ctcgcggtag aggaagagcg gatatacagc agaagcgttt tagccacata
     3421 acagttgttg tagccaaccg agaagaaact agcaatcctc aggagtgagg tgacataagt
     3481 gggtcagaag gtacatcctt atggattcag gcttggtgtc agtaaagatt ggaaagcacg
     3541 ctggataaac gagaagaatt acaaggaata tctcctcgaa gatctcaaga tcagaggctt
     3601 tctcaagaag aactacatgg ccgccggtgt gtcggagata tacatagaga gaccggaacc
     3661 gggcaaagtg gtaatcacta tcaagtgtgc gagacccggt gtaatgatag gaaagaaggg
     3721 aagcgaggtc aagcttctca gacagaagct ggaaaagctt ataaccaggc agtttcagct
     3781 taatatcgaa gaagtgaaaa cacccgaaac cgatgcgata ctcgtcgctg aggacatcgc
     3841 atcaaggata gagaagagag cttcttacaa aagagccatg aaaagagcca tattcacggc
     3901 catgcgtaaa ggggcaaagg gaataaagat catggtctcc ggtagattga acggggccga
     3961 tatcgccagg acagagtggt acctggaagg aagacttcct ctccagaccc tcagagctga
     4021 tctggactac ggttacacca ctgcttttac gaagatgggc atcatcggag tgaaagtatg
     4081 gatctacaaa ggcgacgtgc aggtgtaatt gcaagagggg aggtagttca taatgttaat
     4141 gcccaaaagg gtaaaataca ggaaacaaca gagaggcaag atgaacggaa atgctaaggg
     4201 tggaacgctt gttcactttg gcgaatgggg cataaaggcc ctggagcctc actggataac
     4261 tgctcagcaa ctcgaagcat gccgaatcgc gataaccagg actctgaaaa gaaccggaaa
     4321 cctctggatc cgtgttttcc ccgacaagcc tataacctcg aagggaatcg gcgtgagaat
     4381 gggtaaggga aagggagacg tcgagggctg ggtcgctgtg gtgaagccgg gcaagataat
     4441 gttcgagatc ggcggagttc ccgatcagct tgcaaaagaa gccctggaaa aggcggcttc
     4501 gaagctaccc ataagaacga agatagtccc caggtatcaa ataggaggtg agctctgatg
     4561 aagccggttg aactgcaaaa gttcacagac gaagaactac ttcagatgct cgaagactcg
     4621 aaaagaaagc tgatggacct caggttccag ctagaaatga acaggttgaa gaaccattcc
     4681 cagattactt cggtcaagcg cgatatagcc aggataaaga ctatcctacg cggtcgtgag
     4741 ctggggataa ggaggtaagt ccgccatgcc gaggaaaaca ctgataggaa ctgtagtgag
     4801 caacaaaatg gaaaagacca tagtggtgag cgttacgagg acacacgttc atccatttta
     4861 cggtaagact ataaagacga cgaagaagta ccacgcggac gatccggaag gtctggccgg
     4921 aatgggcgac actgttgaga tcgaagaatg caaacctctc agcaaaatga agagattcaa
     4981 gctgatacgt gtcctgaaga aagatatcta caccggagat attcctgaga ctcccgagga
     5041 cgttgaaggc gctttcggag gtgagaagta atggttcagc tggaatctta cctcaaagtc
     5101 gccgacaatt cgggcgccag ggtcataaaa gtgatccagg ttaccggtgg atacagaaag
     5161 agatcgggaa cgatcggtga cattgttgtc gcggccgtca gagatgcggc acctcacaca
     5221 gacttcaaga aaggcgatat agttagagcg gttctggtga gaacggccaa ggagatacgc
     5281 agaccggacg gcacttacat ccgcttcgac gataacgcag cggtgatcat cgataaacag
     5341 aaccagccca gagggacacg tgtctttgga cctgttgcca gagagatcag agataaaggc
     5401 ttttctaaaa tcgcttctct ggcccaggaa gtctggtgag gagtgatctg aatgagtaga
     5461 gtgaaaaaag acgatctggt catggtcata tctggaaagg acagaggcaa aaagggaaaa
     5521 gtactcaaga catatccttc cgaaaagaaa gtgattgtcg aaggagtaaa cttcaccaaa
     5581 aagcaccaga gaccgacaaa ccagtatcga gaaggcggaa taatagagag ggagtccccg
     5641 atctatgttt ccaaagtgat ggttgtctgc cccaactgtg ataagcctac gagaatcgcc
     5701 cataagatac tcgaaaatgg tgaaaaggta agagcgtgta aaaaatgcgg cgagataatc
     5761 gacaaggttt gatggggagg agtagttgat ggcatacgaa tatatcccat tgaaagagag
     5821 atacgaaaac gaagtgatcc ccgccatgac caaagagttt agttacaaaa acaaactcga
     5881 ggttcccaaa gtttctaaag tcattgtaaa catgggtatc ggcgaaggtt cgaggaacgc
     5941 agatctgctc gagattcacg gaaaagagtt gaccaacata gtgggccaga aacccgtcat
     6001 caccagggcg aagaagagca ttgcgaattt caaactccgt gatggaatgc cagtaggatt
     6061 gaaggtcact ttgagaggcc cgaggatgta caacttcctg tataagctga taaacatcgt
     6121 ccttccaaag ttgagagact tcagaggtgt cgatccagat tcctttgatg ggagaggaaa
     6181 ctacgctctg ggacttcctg aacagctagt ctttcccgag atagtgcccg atcaggtgaa
     6241 gagggtacag ggaatggaca taatagtggt gaccacggcc agaaccgatg aagaggccag
     6301 aaggctcttg gctcttctgg gtgttccttt caaaagggcc atggtataag gaggtaaggc
     6361 atggctaaga aatctattat cgagaagtgg aaacgcgaac ccaagttcaa ggtaagaaag
     6421 tacaatagat gcagcatttg tggtaggccc agatctgttt acagagagtt cgggctgtgc
     6481 agagtctgct ttagacagct ggcttccgag ggaaaactcc ccggtgtaaa gaaagcaagc
     6541 tggtgaggag ggaattgtaa tgtggagtga tcccatagcc gacatgctca caagaatcag
     6601 aaacgcaaat gcggccttca aagagagcgt ggatatacca gcttcgaatc tgaagaagaa
     6661 actactggac attctgaagg ccgaaggtta tatagacgac tacaaataca tcgaagatgg
     6721 caaacagggc gttctgaaag tctttctcaa gtacaaggga gaccgcagga acaaagtcaa
     6781 cgtcatcacc ggaatagtga gaatttcaaa gcccggcaga cgaatctacg tgaacagaga
     6841 caagttgccc aaggttaaat caggaatggg aatagcaatc gttagtactt ccagcggtgt
     6901 gatgaccgac aagcaggcac gcatgaaggg tgtcggcggc gaagtcatct gctatgtctg
     6961 gtaaggaggg atttagatgt cgagacttct gaaacattca atcaaacttc ctgcgggtgt
     7021 tacccatagc ataaaagaca acgtgatcac cgtcaaaggc ccgaagggag aattgaagca
     7081 ggagatcctt cctcttgtca agatcgaggg cgacgaaaaa gagatctggg tcaagacaaa
     7141 cgaagatctg gtcattagaa agagcgatca caagaggctt gccaaatacg cgggtacttt
     7201 ctggtctctc ataaacaaca tggtgctcgg tgttacccag ggtttcgtca aggaactgga
     7261 gatagtcggg gtaggttacc gcgcccagct ccagggaaag aaactggtga tgaacctcgg
     7321 ttacgcccac cctgtcgaga ttgatcctcc gcagggaatt acttttgaag tacctgcccc
     7381 ccaatcggtg gtggtaaagg gtttcgataa gtatctcgtt gggcaggttg cggctaacat
     7441 caagcgctgg agaataccga tcgtttactc cggcaaagga atacgctaca agggtgaggc
     7501 catcaggaca aaagtcggta agaaggtctg atcgaggagg ctgctggaat gtttaagcat
     7561 aaagacaaaa aagaacagag acaaaagaga catcttcgtg ttagggcaaa gctttccggt
     7621 acggccgaaa ggccgaggct ggcagtttat agaagcgaaa agcatattta tgcccagctc
     7681 atagatgaca taaaagggat aactctggtt tctgcttcta ccgttgacag ggaaatgaga
     7741 gactctacag agaaaccgtg gaacgttgaa gctgctagcc aggttggaaa gcttcttgcg
     7801 aagaaagccc tggagaaggg gatttcaagt gtagtctttg atcgtggtgg gttcaacttc
     7861 cacggaagag tcaaatcact tgctgatggt gcccgcgaag gcggactgaa gttctgagga
     7921 ggtgagttgt taaatgtctg aagaaatgat gaacgaccgt ggttcaagac cctcccgtga
     7981 aggcaggaga aacccgagaa cgtccaggaa cgctcctgtc gaaaaacagg tggaagataa
     8041 cgaatttgaa gaaagaataa ttgagatacg aagggttacg aaggttgtgg caggaggaaa
     8101 gaacctttcc ttcagagtcg tcgcaatcgt cggcaaccgt gaaggtaaag tcggcctcgg
     8161 tatcggaagc gcaagggaag tccctatcgc aataagaaag gcagttcttg aggcgaagaa
     8221 gaacatagtc gaggtttcca tcagaaatga gaccgtacct catgaaaccg ttggaaaaca
     8281 ggattcggca aggataatgc tgaagccggc cggtcccggt accggtatta tcgcgaactc
     8341 gccggttcgt gccgtggtag agcttgccgg agtgaaaaac attctgacga agtctctagg
     8401 ttcttcgaac actctgaaca tggccagagc tgcgttgaat ggtctcgtaa gcttgagagc
     8461 ccctcaggaa ttcgcgaggc tgagagatat atctcttaaa gccgtcttcc atggtcttgg
     8521 cgaggaggat ggacagtgat ggcaaagagc ataagagtca gactggtgaa aagtcccata
     8581 ggctataaca gaagacagct caagacagtc aaagccctgg ggctcggaaa gctggacagc
     8641 gagatggttc atcaagatac gccccagatc agaggcatga taaacgctgt tcggcatctc
     8701 ctggctgtcg aagagttggc ggactgaagg gggtaagaag atggctttta acattgacga
     8761 tctaaaaccg actcccggct cgagaaaaag aagcaagaga atagggcgcg gtaccggttc
     8821 aggtatgggt aaaactgcca caagaggtca caagggtcag ggcagggcta caggaagggt
     8881 ttccgctcac ttcgaaggcg gacagacacc tcttttcagg cggatacccg ttaaaggatt
     8941 caagaataaa agcgccgttg aatatgcgac cgtcaacctt tctacgcttg aagagaggtt
     9001 cgagagcgga agcgaagtta caccggaaat actcatgaag atgaagattt tgaagaatct
     9061 caaagacggt gtgaagattc tcgcaagagg ggagataacg aaatccctga atataaaggc
     9121 caacgctttc agcgcttctg cgaaagagaa gattgaatct gctggtggaa aggcagaggt
     9181 gatctgacaa tgtggaatg
//
LOCUS       DAOOLY010000078         9168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8823_length_9168_cov_4.413366, whole genome shotgun sequence.
ACCESSION   DAOOLY010000078 DAOOLY010000000
VERSION     DAOOLY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8823_length_9168_cov_4.413366"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /gene="uvrC"
                     /locus_tag="PLD15_05465"
     CDS             <1..1446
                     /gene="uvrC"
                     /locus_tag="PLD15_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPB63385.1"
                     /translation="LLIGSKVYLYIYLSDDGYPYLEITRIKTRPGTYFGPYTSTGLVR
                     RILELLQRIFGIRGCTFDLSKVKKPCFLYHLKMCSAPCAGKITSEEYANRLESLKEFL
                     EGDTMKVRHALLERMNILSQTLQFERAAEIRDILSSMDQLYAFQGVEASYDLKADVLV
                     VSSGLATLLQVRGGMLLGKLVFDFPDGTPVDFISQFYYAKGNRLPKSLIVYGLNKSDL
                     KPFKKDFSYIGEPRDDQETRLLQIAYQNIDEELKMRLNASHSLKQAQQILGLGKFPSR
                     IEGMDISHTQGLYTVASVVVFDNGKPNKTEYRRYRISGITEPNDFESLATVVKRRYSK
                     HPLPDLLLIDGGEPQVRAVEKAFSEIGIDGYEIVGLAKELNELVFPDKRGRILLPQDH
                     PVLRMIISIDDEAHRFAVNYHRVLREKRYRSSKLDDIKGIGPKRKKALLKAFGGIPGI
                     MEASEEDLYAILKNRKAVEEVRNWIERKGGD"
     gene            complement(1462..1734)
                     /locus_tag="PLD15_05470"
     CDS             complement(1462..1734)
                     /locus_tag="PLD15_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB63386.1"
                     /translation="MNKKELINELSTRTGYTKKVSSDLIDTFVDIVGDKLAEGEEVKL
                     VGFGTFEVVERKPRKGVNPRTKKAIKIPGAKVPKFRAGKELKEKVQ"
     gene            complement(1861..3186)
                     /locus_tag="PLD15_05475"
     CDS             complement(1861..3186)
                     /locus_tag="PLD15_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPB63387.1"
                     /translation="MKKRIVVNLPVEELPKSWYNVKADLPFKMDPPLNPVTKEIMNPA
                     DMTAIFPKAIVEQEISEERFIEIPEPVLNEYAVFRPSPLIRATFLEEFLKTPARIYYK
                     YEGNSPTGSHKTNTAIAQAYFNRAEGVTELTTETGAGQWGSALSYAGRKFGITVRVFM
                     VRNSYETKPMRQHMMRMFNGTVEPSPGENTESGRNFLKNDKNYPGSLGMAISEAVEIV
                     LNSKDKKYSLGSVLDHVLLHQTVIGLEIEKQLEILNEKPTVILGCHGGGSNFGGTVLP
                     FVKRKLEGEKIEFVACEPESCPSLSKGEFRYDFGDSAGLTPMVKMYTLGREFVPPTIH
                     AGGLRYHGAAPIISRLVKEKIVTPENFPQEETFKAGILFATTEGIIPAPESSHAIAGA
                     IRRAISARERREEEVIVFTLSGNGLLDLSSYTSIIESNEKILELEGVKG"
     gene            complement(3464..5125)
                     /locus_tag="PLD15_05480"
     CDS             complement(3464..5125)
                     /locus_tag="PLD15_05480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB63388.1"
                     /translation="MKKAILIVFLAIFSITILAGGYVAQLTRAGVTLSEEFWISSEEL
                     QQALEATIANARSQGVELDPYFDNMYLPSEIALKLMLVSYIVDEKVVDIYALENSLLP
                     DESEVESETDAMLSQYASSPEMVAQIEAIYGSMDNFRKEIRNYIYQSLKAVVVQDNIA
                     PLNEEALSTYFEQNKENLKNSYEYIRPRHILLSDEATATGLKNRINAGEITFAEAALQ
                     YSIDSGTAANGGQLGTIQRGQTVAEFEEAILTAPIGVLYGPVKSEFGYHLIIVEERKS
                     IESLNDIVSDPQIYNDFVSAYQNDTYYAWIEKYIADNAFDYEILDPELLLYDDYTKVM
                     NDENGATAMVVTLATKLFGPSATENPLPVEYAVFIQLSDSLALTESPNYRLAIERLYE
                     IGEKRGMIMELIFQLKGDDPAVATDYYNGWLVELENIFADPQLLQTQLSQYGESFVQY
                     VFSTIDQIDAVLGQLLAGEMKVETRKTALDVLLRNNSLALDLNYDPVWQKEKLTSRLA
                     YLKDYASIEPSGDIQYEIDSIASQLEGLETQDSTPTDTQEATSTN"
     gene            5197..5589
                     /locus_tag="PLD15_05485"
     CDS             5197..5589
                     /locus_tag="PLD15_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63389.1"
                     /translation="MKVLEKKGLNLESFLSTSWKWLNSEEAELAGGEGSRQPQAIYFF
                     ADEQNRSFCRRYNREVNFKRDVEDIGLCDKCGMRFWNYRAAQICVFAPAEGYYTIDSR
                     NEGKETTEFPDKEVALSGISSFESKSWE"
     gene            complement(5617..6978)
                     /gene="lpdA"
                     /locus_tag="PLD15_05490"
     CDS             complement(5617..6978)
                     /gene="lpdA"
                     /locus_tag="PLD15_05490"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPB63390.1"
                     /translation="MYDAIVIGGGPGGYVCAIRLSQLGKKVALVEKEYLGGTCTNWGC
                     IPTKAMLTSAHLFSEIKDKSKRLGVDVSDLNYRLDGIMSHMNRTVTTSRKGIELLLKK
                     NGVDFFNDKAVVKDPHNVLLEKNNSLLEAKNIVLANGSEPSMFPPFSKVEGMWTSNDV
                     FKLAEMPSSLAIVGGGVIGVEFATFFSTFGVKTTIVELADHILPYEDTDVAEEIRKVL
                     KKKGVTILEKTKVVSVEKLDKGYLLNTTGDEEITIEAEKVLVAVGRRAVLSDDIKNLG
                     LEIVRGVVTDRRMKTSVQGVYAIGDIRAGIMLAHVASYEGIVAAHNIAGEELEMDYSA
                     VPSIIFSSPEVASTGLKESDLKDRSDVVVSRFPLSANGRARTILESSGFVKVLAEHGT
                     GRVLGMSIVSPSATDLIMEAVIAVKNGLTVEQLENSIHPHPTLSETVLGALEGIEGKS
                     IHI"
     gene            complement(6980..7549)
                     /locus_tag="PLD15_05495"
     CDS             complement(6980..7549)
                     /locus_tag="PLD15_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPB63391.1"
                     /translation="MNLKLPDLFKVFANQTRIEIATLIVDNCMTASEISERLQTDLST
                     IYRHLQHMKKLGILNSNFSKGVERFDFSSPRIYRLLEEAIAFISEKKEYRSLDCSEGI
                     CRYYLGETGVDVKPDLLLDMRGESCPVPDMQTKVTLSEMEEGKVLLVIVDYPLSGERI
                     PVFVQREGHEFLKKIVDDYGDIKIYIRRK"
     gene            complement(7626..7889)
                     /locus_tag="PLD15_05500"
     CDS             complement(7626..7889)
                     /locus_tag="PLD15_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrH/TusB family sulfur metabolism protein"
                     /protein_id="HPB63392.1"
                     /translation="MALIVIKYGKNNVAETIKFKAARKGDCVVLVQDGIFWALDDIET
                     EAEVFAVIDDATARGYSAEELTVPSITYEGFVEIIEKCEKTIG"
     gene            complement(7900..8253)
                     /locus_tag="PLD15_05505"
     CDS             complement(7900..8253)
                     /locus_tag="PLD15_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular sulfur oxidation protein"
                     /protein_id="HPB63393.1"
                     /translation="MKKILFVVYQAPGGSIWVNEAFRTAFGMYGEDIEPSVLLVEEAT
                     VALSKKAQPECLGLLPISICFRFIKRYETEVYGIKEHVEKFKIKDIEESFSVKLIDEK
                     DTGDFLHDFDNVIFM"
     gene            complement(8264..8620)
                     /locus_tag="PLD15_05510"
     CDS             complement(8264..8620)
                     /locus_tag="PLD15_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPB63394.1"
                     /translation="MKITIQVFVPPYTYEDLDTAIKLAEAALNRGHDVTIFLFADSIL
                     SINGSVKPIRVDRNIPEKLKTLIAEKGLKVEICGICMDYRGVTKDMIIPGSNPSGLPE
                     LATLIYSSDRFVNLMA"
     gene            complement(8631..>9168)
                     /locus_tag="PLD15_05515"
     CDS             complement(8631..>9168)
                     /locus_tag="PLD15_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HPB63395.1"
                     /translation="PTLATIFNINPWIPAAIVAALLLWYTLGTKTTDRPTKFNWKVAA
                     IALAILSPIAWITSEAAGRNYGFGITGGWISIFDTFISNQPLRWDGYEIISIILGALI
                     ASLLAKEFKLRMPKNPKTYLQVMVGGIMMGAGANLAGGCNIGHFLAGLPTLAISSIVA
                     SIFLILGNWTMAYILYRK"
BASE COUNT         2215 a         2315 c         2125 g         2513 t
ORIGIN      
        1 ctgcttatag gttcgaaggt ctatctttac atctacctct ccgacgacgg gtatccctac
       61 ctggaaataa cgaggatcaa aactaggcct ggcacctact tcggacccta taccagcacc
      121 gggctggtca ggcgtatcct ggaattgctt cagcgaattt tcggcatcag gggctgcacg
      181 tttgatcttt cgaaggtcaa aaagccctgc tttctgtacc atttgaagat gtgttcagct
      241 ccttgcgcgg ggaagataac atcggaagag tacgctaacc ggctggaatc tctcaaagaa
      301 ttcctggaag gagacactat gaaggttcgt catgctctgc ttgagagaat gaacatcctg
      361 tcccaaactc tccagtttga gcgcgcagca gagataaggg atatattgag ttcgatggac
      421 cagctttatg cctttcaggg cgtggaggcc tcttacgatc taaaagccga tgtattggtc
      481 gtttcatcag gactggcgac gcttctgcag gttcgcggtg ggatgttact tggaaagctc
      541 gttttcgatt ttccagacgg cactccggta gatttcatat cgcaattcta ctacgcgaaa
      601 ggtaatagac ttccgaaaag tctcatagtt tatgggttga acaagtccga ccttaagcct
      661 ttcaagaagg acttctcgta catcggagaa cccagggacg accaggagac caggttgctc
      721 cagatagcct atcaaaacat cgatgaagaa ttgaaaatgc gcctgaacgc atcgcattcg
      781 ctgaagcagg cgcagcagat tctgggtctg ggcaaattcc cctcccgtat cgaaggcatg
      841 gatatctcgc acactcaggg gctatatacg gtggcatcgg tcgtcgtttt tgacaacggc
      901 aaaccgaaca aaaccgagta cagaaggtac aggataagcg ggatcaccga accgaacgac
      961 ttcgaatcgc ttgcaacggt agttaagcga cggtattcga aacatcctct gcccgacctc
     1021 ctgctgattg acggcggcga acctcaggtg agggccgtcg aaaaggcctt ttcggagatc
     1081 ggaatagacg ggtacgaaat cgtcgggctc gccaaagagc tcaatgaact ggtcttcccc
     1141 gacaaaagag ggagaatact gcttccgcag gaccatccgg tgctcagaat gatcatctcg
     1201 atagacgatg aagctcacag gttcgccgtg aattatcaca gggtattgag agagaagagg
     1261 taccgaagtt ccaagctcga cgatataaag ggaataggtc caaaacgcaa aaaggctctg
     1321 ctcaaagcgt tcggaggtat cccgggaata atggaggcct cggaagaaga tctttacgcg
     1381 attctaaaga acagaaaagc ggtagaagaa gttcggaact ggatagaaag aaaaggtgga
     1441 gactagctcc accttctatg actattgcac tttttccttg agttctttcc cggctctgaa
     1501 ctttggaacc tttgcaccgg ggatctttat tgcctttttt gttctcgggt tgactccctt
     1561 tcgcggcttt ctctccacca cttcgaaggt gccaaaaccg actaacttga cctcttctcc
     1621 ctcagcgagc ttatcgccga cgatatcgac gaaggtatcg atcagatcgc ttgaaacctt
     1681 cttcgtataa ccggtcctcg tggaaagctc gttgataagt tccttcttat tcacgtaaac
     1741 accccctggc tagaattcag atgtcatcca tattctaaca tcttatggag aagtattgca
     1801 agggctgtgt tgcgcaaatc ctctaaataa aatacattca gaaaacccgc tcttttccgt
     1861 tcaacctttg accccttcaa gctcgagaat tttctcgttg ctttcgataa tgctcgtata
     1921 agatgagagg tccagcagtc cattaccgct cagagtgaag acgatgactt cttcctctct
     1981 tctctctctg gcgcttatgg ctcttctgat cgctccggct atggcgtgag atgattccgg
     2041 agccggaatg atgccctcgg ttgtcgcgaa gagtatcccg gccttgaagg tctcttcctg
     2101 ggggaagttt tccggtgtga ctatcttctc tttgaccagt ctggagatta taggggccgc
     2161 cccgtgatac ctcagtccgc cagcgtggat ggtcggcggc acgaattccc tgcccagtgt
     2221 gtacatcttc accataggcg tcaagcctgc gctgtcaccg aaatcgtacc ggaactcgcc
     2281 ctttgaaaga gacggacagg actccggttc gcaggcgacg aattcgattt tctctccctc
     2341 gagctttctt ttgacaaatg gaaggaccgt acccccgaag ttcgagccac caccgtgaca
     2401 gccaagtata acggtcggtt tttcgttcaa gatctctaac tgtttttcga tttcgagtcc
     2461 tatgacggtt tgatggagga gtacgtgatc gagcacacta ccgagcgagt actttttgtc
     2521 tttggaattc aatacaattt ccaccgcttc gctgatggcc attccaaggg aaccgggata
     2581 gtttttgtcg ttcttgagga agtttctgcc actctctgtg ttctcgcctg ggctcggttc
     2641 caccgtaccg ttgaacattc tcatcatatg ctgtctcatg ggcttggtct cgtaactgtt
     2701 ccttaccatg aagaccctca cggttattcc aaactttctt ccggcgtaag agagagcact
     2761 gccccattga ccggcgcctg tttctgtcgt cagttcggtg accccttcgg ctctgttgaa
     2821 ataggcctgc gcgatggctg tgttggtttt atggcttccg gtgggggagt tcccttcgta
     2881 tttataatat attctggcag gggttttaag gaactcttcc aggaaagtag ctcttatcaa
     2941 cggacttggg cggaacacgg cgtattcgtt taatactggc tcgggaattt cgatgaacct
     3001 ctcttcgctt atctcctgtt cgacgatggc cttaggaaaa attgccgtca tatcggcagg
     3061 attcataatc tcctttgtca ccgggtttag aggtggatcc atcttgaaag gcagatcggc
     3121 cttcacgttg taccagcttt ttggaagttc ctcaacaggt agattaacca cgattctctt
     3181 tttcatgcct ttacctccta aaaaataaag gggctctcat cacgagagcc ccttcaatgg
     3241 gtttaccttt atagcaaaag gctaaccccc attaaagggg ctaacccacc accagaactt
     3301 attcagtatg tatgaactca aatggttaat ttcatcaatt ctacttcgca tgattaacta
     3361 tactattttt taccgtcttc agtcaagcga tgatacgtaa agattacata aaaacgggga
     3421 tctcttttta cgaggtcccc gccatagaat tattctaatc ctgtcaattt gtggatgtgg
     3481 cttcttgggt atcagttggt gttgaatcct gggtttccaa accctcaagt tgagatgcga
     3541 tgctatctat ctcgtactga atatcgccgg aaggttctat ggacgcataa tccttcagat
     3601 atgcgagcct tgaggtaagc ttctctttct gccagactgg atcataatta agatcgagag
     3661 ccagactgtt gtttctcagc agaacatcga gagcggtctt tcgagtctcc actttcattt
     3721 caccggccag aagctggccg agaaccgcgt cgatctggtc tattgtactg aaaacatact
     3781 gaacgaaact ctccccatac tgactgagtt gcgtttgaag gagctgagga tcggcgaaga
     3841 tgttctctaa ctcgacaagc cagccgttgt aataatcggt tgctactgcg gggtcatctc
     3901 ctttgagctg gaaaatcaac tccattatca ttccacgctt ctcaccgatc tcgtaaagtc
     3961 tttctatcgc cagcctgtag ttgggacttt ccgtcagggc cagagaatcg gagagctgaa
     4021 tgaagaccgc gtactccacc ggcaacggat tttcggttgc cgaaggtccg aagagttttg
     4081 tcgccagagt cacaaccatc gccgttgcac cgttctcatc gttcatgacc tttgtgtaat
     4141 catcgtacag gagcagctcg ggatcgagta tctcgtagtc gaaagcgtta tctgctatat
     4201 acttttcaat ccaggcgtag taggtatcgt tctgataagc ggaaacaaaa tcgttgtaga
     4261 tctggggatc ggaaacgata tcgttcaacg attcgatcga ctttcgctct tcgactatta
     4321 tgagatgata accgaattca ctcttaaccg gtccgtacag tacgcctatg ggggccgtca
     4381 gtattgcttc ttcaaactcc gcgacggtct gacctctctg aatagtgccg agctggcctc
     4441 cgttggcggc cgtaccggaa tcgattgaat actgaagggc tgcctcggcg aaagtgattt
     4501 caccggcgtt gattctgttc ttgagaccgg tcgcggtggc ctcatcgctc aagagaatat
     4561 gtctgggcct tatatattcg tagctgtttt tcaggttttc cttgttttgt tcgaaatagg
     4621 tcgacagtgc ctcttcgttc aatggtgcta tgttatcctg aacgacaacg gcctttagag
     4681 actggtaaat gtaatttctt atctcctttc tgaaattatc catcgacccg tagatcgctt
     4741 ctatctgtgc aaccatttca gggctcgatg catattgaga aagcatagcg tcggtctcgc
     4801 tttcgacttc gctttcgtct ggcagcaacg agttctccag agcatagata tcgacaactt
     4861 tttcgtcaac gatatatgaa accagcatga gcttcaaagc gatctcgctc ggcagataca
     4921 tattgtcgaa gtaaggatcg agctccacac cctggctacg agcgttggct atcgttgctt
     4981 cgagggcttg ctgaagctcc tcagatgata tccagaattc ctcggacaga gttacgccag
     5041 ccctggtgag ctgggcgacg tatccgccgg ccaggatcgt tattgagaaa attgccagaa
     5101 aaactatcag tatcgctttt ttcaaaatga ttccccctcg tgtatttggt atattcatga
     5161 taacatttca ttgggttttg cgtgtcacaa ttagaggtga aagttttgga aaagaagggt
     5221 ttgaatcttg aaagttttct ctcaacttcc tggaaatggt tgaatagcga ggaagcagaa
     5281 cttgcagggg gtgagggatc tcgtcaacct caggcgatat actttttcgc cgatgaacaa
     5341 aaccgctcct tctgcaggag gtacaacaga gaggtcaact tcaaaagaga cgtcgaagat
     5401 ataggtctct gcgataagtg cggtatgagg ttctggaatt acagggccgc tcagatctgt
     5461 gttttcgctc ccgctgaggg gtactacacg attgactccc ggaatgaagg taaagaaaca
     5521 accgagtttc cagataagga agtagccctg tcaggtatat ccagttttga gagtaaaagc
     5581 tgggaataaa ttaagaagag gcctttcggc ctcttctcat atatggatac tcttgccctc
     5641 tattccttca agcgcaccca ggacagtttc tgacagagtc ggatgtgggt ggattgaatt
     5701 ctccagctgt tcaacggtca atccgttctt cacggcgatc acggcctcca ttatcagatc
     5761 agtggccgag ggagagacta tactcatacc caaaaccctt ccggtgccgt gctcggccag
     5821 tactttaaca aagccggagc tctctagtat cgttcgtgcc cgtccgttgg cgctcagggg
     5881 aaatctggag acgactacat cggatctatc cttgagatcg ctctccttca agccggtaga
     5941 ggcaacctct ggactggaaa agattatcga aggaacggcg gaatagtcca tttccagctc
     6001 ttcaccagct atattgtgtg ccgccactat cccttcgtaa cttgcaacgt gtgcaagcat
     6061 gataccggcc ctgatatcgc cgatcgcgta aacgccctgt acgctcgtct tcatacgcct
     6121 gtccgtaacg acgcccctta caatttccaa gcccagattc tttatgtcat cggagagaac
     6181 ggctctcctg cctaccgcga ccagaacctt ttcggcctcg attgtgatct cctcgtcgcc
     6241 agtcgtattg agaagatagc ccttatcgag cttctcgacg gatacgacct tggtcttttc
     6301 gaggatggtt acaccttttt tcttcaggac cttccttatc tcctccgcca catcagtgtc
     6361 ttcgtagggc agaatatgat cggccagctc gacaattgtc gttttgactc cgaatgtact
     6421 gaagaaagtg gcgaactcaa ctccgatgac tccgccgcca actatggcaa gactcgaagg
     6481 catctccgcc agtttgaaca catcgttgct cgtccacatt ccttcgacct tgctgaatgg
     6541 agggaacatg gatggttcgg aaccgttggc aaggactatg ttttttgcct ccaacaagga
     6601 gttgttcttt tcaaggagta cattgtgcgg gtctttaaca acagctttat cgttgaaaaa
     6661 atcgacaccg tttttcttta gcagcaactc gattcccttg cgtgaagtgg tcaccgtcct
     6721 gttcatgtgt gacatgattc catcgaggcg gtagttgaga tcggaaacat ccacgcccag
     6781 tctcttcgac ttatccttta tctcggaaaa caaatgggcc gaggtcagca tggccttcgt
     6841 cggtatgcaa ccccaatttg tgcaggtgcc tcccaggtat tccttctcca caagcgccac
     6901 tttctttccc agctgggaca atcttatggc acagacgtac cctccgggtc ccccccctat
     6961 aacgattgca tcgtacatct catttcctcc ttatataaat ctttatgtcg ccgtagtcat
     7021 cgacgatttt cttgagaaac tcgtgacctt ccctttgaac gaatacggga attctctcgc
     7081 cggaaagcgg gtaatccact atgaccagta gaaccttacc ctcttccatc tctgagagag
     7141 tcactttcgt ctgcatatcc gggaccgggc aggactcacc gcgcatatcg agaagaagat
     7201 cgggttttac atctacaccc gtctccccga gataataacg acaaatacct tccgaacagt
     7261 ccagggacct gtactccttc ttttcgctga tgaaggctat cgcttcttcg agcaatctgt
     7321 atatcctggg agaactgaag tcgaatctct cgactccctt cgagaagttt gagttcaata
     7381 ttcccagttt tttcatgtgt tgaagatgtc tatagattgt ggaaagatcc gtctgaagtc
     7441 tttccgatat ctccgaagcg gtcatacagt tatcaacgat cagagtcgca atttctatcc
     7501 tcgtttgatt ggcgaagact ttgaatagat caggtaactt gagattcatc atagaaatcc
     7561 actatcacct ttcgaagaaa ggtgcccttc caggaagagc acctctcgga tttcaaaatc
     7621 ggcaactaac cgatagtttt ttcacatttt tcgattatct ctacaaaccc ctcgtaggtg
     7681 atcgagggaa cggtcagctc ttcggcgctg taacctctgg ccgtcgcatc gtcgataacc
     7741 gcgaaaacct cggcctcggt ttctatatcg tctaaagccc agaatatgcc gtcctgaacc
     7801 aggacaacgc agtctccctt ccttgcggcc ttaaacttta tagtctctgc aacgttgttt
     7861 ttgccgtatt ttattacaat aagggccata tcaaacacct cacatgaaaa ttacgttatc
     7921 aaagtcgtgg aggaaatcgc ctgtatcttt ctcatcgatt aatttgacgc tgaaactctc
     7981 ttcaatatcc tttattttga acttttccac atgttctttg ataccgtaaa cctcggtctc
     8041 gtacctcttg atgaatctga agcagatcga tatcggaagc agaccgagac attccggctg
     8101 ggcttttttc gacagtgcga cagtcgcctc ttccaccaga agcaccgaag gttcgatatc
     8161 ttctccgtac atcccgaacg cagttctgaa ggcctcgttt acccatatgg agcctcccgg
     8221 cgcctgataa acaacaaaaa gtatcttctt catcaccatc acctcatgcc ataaggttca
     8281 cgaacctgtc gctgctataa ataagggtcg caagctcggg cagtccgctg gggttggagc
     8341 cggggattat catatccttg gtaactcccc tgtagtccat acagattcca cagatctcca
     8401 ccttcaaacc tttttcggct atcagggtct tcagtttctc cggaatgttt ctatcgactc
     8461 tgattggttt cacagaaccg tttatcgaga gaatagaatc ggcgaacagg aatatggtta
     8521 catcgtgacc tctgttcagg gccgcttccg ctaacttgat cgccgtatcc agatcttcgt
     8581 aagtgtacgg aggaacgaat acctgtattg ttatcttcaa aacttccgcc tcactttctg
     8641 tacaatatgt acgccatcgt ccagttaccg agaatcagga agatgctggc aactatggat
     8701 gaaatcgcaa gtgttgggag accggccagg aagtgcccga tgttacagcc accggccaga
     8761 ttggcaccgg cccccatcat tatacctcca accatgacct gaagatacgt ttttgggttt
     8821 ttcggcatcc tcagtttgaa ctctttggcc agaagagacg cgatcaaggc accgagtatt
     8881 atactgatta tttcatagcc atcccatctc agaggctggt tggatatgaa ggtatcgaag
     8941 atagatatcc agccgcccgt tatcccgaaa ccatagttcc tgccagccgc ttcgctcgtt
     9001 atccatgcga taggcgaaag tatggcgagc gctatcgcgg ccactttcca gttgaacttc
     9061 gtcggtctat ccgtggtctt cgtacccaga gtgtaccaga gcaacaaagc cgcaacgata
     9121 gctgcaggta tccatgggtt aatgttgaat atcgttgcca gtgtcggg
//
LOCUS       DAOOLY010000079         9118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_8897_length_9118_cov_4.744014, whole genome shotgun sequence.
ACCESSION   DAOOLY010000079 DAOOLY010000000
VERSION     DAOOLY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_9118_cov_4.744014"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLD15_05520"
     CDS             <1..429
                     /locus_tag="PLD15_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPB63396.1"
                     /translation="TDLDLFIEPGRSMVGNSAILVSQVILRSRKGDEDWVYIDAGVFH
                     GLTETIEGFRYEVLTEGKVDDTKISFHLAGPTCDSVDTVYHEIDLPKNIGYGDIVYFI
                     NAGAYTTEYATNFNGIEAPRVYFVEDFVDAQMPIEGDFAE"
     gene            complement(455..1303)
                     /locus_tag="PLD15_05525"
     CDS             complement(455..1303)
                     /locus_tag="PLD15_05525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB63397.1"
                     /translation="MNRYRNSTIVLIVINVAVYLFVAILGFFKGPGGISLRDLITLYG
                     GVSRYALSSGLIYTPVTALFLHGNFMHILFNMWALFQLGMVVEAVYGKKLYLLFYFVT
                     GIAGGLSAAAFSPALTIGSSSAIFGLVGILFTLGLKKDTPVMLRSITGYSLLPIILIN
                     LFLGFSIPGISNAAHIGGLIAGAAIGWFAKPAYARFGRQKRVYTRVTEKSSQEVSQEI
                     LIKYLPILNALKNGDSEERTVRLAQLRSELSNLRDEEVASKVLWELFKRDLISQEEFE
                     KLRKFL"
     gene            complement(1313..2233)
                     /locus_tag="PLD15_05530"
     CDS             complement(1313..2233)
                     /locus_tag="PLD15_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63398.1"
                     /translation="MIRLPSYLFFIGGFFSYASIFFASPAVSMTMSIIGMIISLYIWY
                     VLARNRDIHLKIMKVRKLIAEENLRNLKIYPNARLWVILYSASFIVMNISGLFVIKAI
                     IDNVDVTLEAPRMEELIEMLGTGYVLFSWVFFLSGIASILLYAKLIVLLYNDEMKIQS
                     LEGKARNIPLLVTKPLSVILVLLFTLVTYGLFSWFMRYRLSSFQKLHNFFEKKLDSES
                     MKLVSLQERGQDREVKSESEELAKDLLKKYSESLGRVNGPEDRKEVIALLFKDLGDLK
                     TDQARSLLDQLLSKELLSENEFNRLIRLLV"
     gene            complement(2230..3912)
                     /locus_tag="PLD15_05535"
     CDS             complement(2230..3912)
                     /locus_tag="PLD15_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPB63399.1"
                     /translation="MIFDGLVLKRVVNGLQNIVGQQLRQIYQKGKSEFFFKFSRSGLN
                     VSLDPSTPYISFGEKDESSSLETPFSIYLRRHLNGLFLESITQEKTDRIVRLILEGGN
                     AFGMREIYQLVIELMGPGSNMIVLDGKGTVTHAFREMITEKRTLARGVRYVPPEPRGK
                     DLSSLSDGEIRSSMLVSGEKLPKAIWSTFSGISKRTSHNITGYLGLEEVAPDELEDEK
                     LDELCTFLGKIASDFNSSILYTCLNEGEMEISPIPLDFKGDCKAMQASMVINEALVHF
                     GVDKEIDRRRRSIVGKLLKAIARQEKLIEKLERELQEVNDYDRYRYYGELLVANLYRL
                     KDRTPSIELEDWESGERIRIDLDDRLTPSENAQLFFKYYDKSRRKEIQVTRRLKELNG
                     EREYLEQLEEMVSLSENLVELDSFKDELRQAGIIRAESVEKKRKRTPPSGPRIFERHE
                     FKYLVGKNNTQNDEITKAASKEDFWFHARGIPGAHVILKKAGKDVPEKAIRFGAALAG
                     RYSKGRFSGKVEVVYTTAGNIIKPKGAKPGMVVYRKAKTILVDLSTELEEGL"
     gene            complement(4140..4982)
                     /gene="ispE"
                     /locus_tag="PLD15_05540"
     CDS             complement(4140..4982)
                     /gene="ispE"
                     /locus_tag="PLD15_05540"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPB63400.1"
                     /translation="MVADRNEPVEITLKCPAKINLFLEVGQKREDGYHEILTIFQTID
                     LYDELILHPGVPTGYFRSNIDLAWNGSNTLYKAVKAVEKHLGKELALGMELKKKIPAG
                     GGLGGGSSDAAALLRYLGETFHIEREALFEVACSIGSDVPFFLRGGTAIGTGRGEVLT
                     FPGDLEDYSVDLCFPGIGVSTPLAYTLLGRSADGHELGNKEVEKLLSALRERDLTLIG
                     RLSKNDFEGPVFAMFPEIGRAYDKFKEKREAIATRMTGSGSTLFSLFEGTKGEYRFIT
                     STDG"
     gene            complement(4949..5521)
                     /locus_tag="PLD15_05545"
     CDS             complement(4949..5521)
                     /locus_tag="PLD15_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPB63401.1"
                     /translation="MKEKVLIVDVDRLGELATRNGLLALPLECIKKPVELYGRFVPRE
                     EAESDESGRQIIPYVILRDEKRYLLMRRTNRQGESRLHDKYSIGVGGHINPEDGRNLW
                     QAFERGMRREIDEEVKVDISSLEYLGILNDLSTAVSRVHMGIVYIASVVFHGFNEKDK
                     FTGSMVEFEELEMYREKMETWSQIVMSRLK"
     gene            complement(5535..6656)
                     /locus_tag="PLD15_05550"
     CDS             complement(5535..6656)
                     /locus_tag="PLD15_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63402.1"
                     /translation="MKRFRTLLVISFIAAIGLLVSSCWPMLPSISLTIQGSNREPGKP
                     IMVEAMNFRFAPNAAGYIWKLEKELGGGGWQDITAQNLKIMKSTRFKDDTVVVYIPTV
                     PETGRVKITARATGQDDDGALVTVEYSQTVTYNPKTSVLVEVFEKAGTTFGQPSSWFG
                     AFSFSWSNRVPNFVRYKEGFLESAVGLDPYAQRLLFGIDPTSAGAIYNAGEVFFQAWT
                     KLPSNTYTSAQMSTMFNNAPTTGDWAIRIEPTTFSGWRATGWKVKVQNIDRFTDDLWL
                     FRARKSDNRATHLVKINSYYVPGAVEEFVEEDIDTFVSFAFSEGNLLPPGSRLWDENY
                     YFGVIVVQKTAPSSQYNEEDVIFTTLKGSAMVLFPNKSW"
     gene            complement(6657..>9118)
                     /locus_tag="PLD15_05555"
     CDS             complement(6657..>9118)
                     /locus_tag="PLD15_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB63403.1"
                     /translation="LSSRGLLLSFYKTGELKLERLNSLIVVDTSDIQRLPFSIRRLIT
                     AGTEVKFFDHHLQATGEKPGTYRDLGACTTLMCTLLRKKKKHITQNEASLFATAIYRE
                     TGNFTHANTKPQDLRTAAWLLDSGVHPDELGVYSSYRLSLSQQKLVETLLLNIETVDV
                     GGVEISFARAQRDSLPSGMSLAIEKLWSFLGVENLVVLVKVGERIYFTIRSRHMNLDL
                     NGLGGSLRNGVGAFTMGYFEEGDFEKADLAIRGAFEKNIERLIKVRDIMSSPVRTVLT
                     EMRVEDVLKIMQRTGHHTLPVVDQDKIVGIARHRNVEKASRHNLGDRKIVEIMDPFFV
                     VASANDSVQSVTDRMVENDTTSALVLENGILTGIVTRTDLLKSPFSRQRFLQSREQKE
                     DQNYVKLSIARLLEERVEGRLLTMLRFLGAVGSELGMPTYIVGGFVRDLLLNKTNLDL
                     DIVVEGNANSFAAAFKKYFDIKIVEHKEFLASSMFFHDGLRIDVATARTEFYKRPAAL
                     PEVDMSTIKKDLYRRDFSINAMAVKLNQEEFGMLIDFFGSRNDLARGTIRALHPLSFI
                     EDPTRVLRAVRFEQRFGFEIEVRTAELLKNCVREDYLDKVAGQRLRDELNKILLEPQP
                     LKAIRRLSTLGVIEKLFPGVVFDRRIDFAIEKYFLRRPRNNGYIGEDRILYTLMMILL
                     RYSINEQVQWNIQRYGLPRNFLERLRDARENAESISRENPEKPSQYHCAIKSKKAETL
                     NFLNSQLEDERDRSFVEYLKRLKETKLAVDGERLKRAYGLKEGPEIKKVLDALYCARL
                     DGLEIEKEDGFVKDFLRMEGI"
BASE COUNT         2026 a         2560 c         2142 g         2390 t
ORIGIN      
        1 accgatctgg atctctttat cgaaccggga aggtcgatgg tgggtaattc ggccattctg
       61 gtaagccagg tgatacttcg aagcagaaaa ggagacgaag actgggttta catcgacgcg
      121 ggagttttcc atggcctgac ggagactatc gaaggcttca ggtacgaggt cctgaccgag
      181 ggaaaggtcg acgataccaa gatctccttc cacctggccg ggccgacatg cgattccgtg
      241 gataccgttt accacgaaat cgatctcccc aagaacatag gttatggaga tatcgtttat
      301 ttcatcaacg ccggtgctta caccacagaa tatgcaacca atttcaacgg catcgaagcc
      361 ccgagggtct attttgttga agacttcgtg gacgcacaga tgccaataga aggcgacttt
      421 gcagaataag agcccctttc ggggctctta ttttctatag aaactttctc agcttctcga
      481 attcctcttg cgatatgaga tccctcttga agagctccca gagaaccttt gaagccacct
      541 cttcatctct gaggttgctc aactcgctcc tcaactgggc caacctcacg gttctctctt
      601 cggaatcgcc gttcttgagc gcgttcagaa tcggcagata cttgatgagg atctcctgcg
      661 agacctcctg ggaacttttt tccgtaaccc tggtataaac tctcttctgt cttccaaagc
      721 gtgcataggc cggctttgcg aaccagccga tggccgctcc ggcgatcaat ccgcctatat
      781 gggcggcgtt gcttataccc ggaatactga aaccgaggaa gaggtttatc agaattatgg
      841 ggagaagcga atagccggtt atcgacctca acatcaccgg ggtgtccttc ttcagtccca
      901 gagtgaagag aattccgacc aacccgaaga tcgcgctgct cgagcctatg gtgagggccg
      961 gcgaaaaagc cgcggcgctg agccctccgg cgatacctgt cacgaagtag aagagcagat
     1021 acagcttctt cccgtaaacg gcctcgacaa ccataccgag ttgaaagaga gcccacatgt
     1081 tgaaaagaat gtgcatgaaa ttcccgtgaa ggaacagcgc cgtcaccgga gtgtagatca
     1141 atccgctgga gagcgcgtac ctgctgaccc cgccgtaaag agttatgaga tcgcgcaggc
     1201 ttataccgcc aggacctttg aaaaagccaa gtatcgctac gaaaagatac acggcgacgt
     1261 ttatgactat caagactatc gtagagtttc tgtatcgatt caaaataccc tcctatacca
     1321 aaagtcttat caacctgttg aactcgttct cgctaagcag ctccttcgaa agcaattgat
     1381 cgagaagcga tctggcctga tcggtcttaa gatctcccag gtctttgaag agcaaggcga
     1441 tcacttcttt gcgatcttcc ggtccattga cacgacccag gctctcggaa tattttttca
     1501 gcaggtcttt ggctaattcc tccgattcac tttttacttc cctatcctgt cctctctcct
     1561 gtaaactgac caatttcata gactcgctgt cgagcttctt ctcaaagaag ttgtgcaact
     1621 tttgaaagga ggatagcctg tacctcatga accagctgaa aagcccgtag gttacgagag
     1681 tgaagagcaa cacgagaatc accgaaagcg gtttggtgac taatagcggg atgttcctcg
     1741 cttttccctc gagagattgg atcttcatct cgtcgttgta cagtagcact atcagcttgg
     1801 cataaagaag gatcgaggct attcccgaca gaaagaagac ccagctgaag agcacataac
     1861 cggttccgag catctcgatc aattcttcca tcctcggagc ttccagagtt acatcgacat
     1921 tgtctattat cgcctttatc acgaacagtc ccgagatgtt catcactatg aaagaggccg
     1981 aataaaggat gacccacagt ctcgcgttcg gatagatctt cagattcctc aggttttctt
     2041 cagcgatcag ttttctgacc ttcattatct tgaggtgtat atctctgttg cgggccagaa
     2101 cgtaccatat gtagagactt ataatcatgc cgatgatcga catcgtcata gagacggcgg
     2161 gactggcgaa aaagatcgaa gcgtaagaaa aaaagcctcc gatgaagaac aaataagacg
     2221 gaagtcttat caaagccctt cctccagctc cgtcgaaaga tcgactagga tagtttttgc
     2281 cttcctgtaa accaccattc ccggcttcgc tccctttggc ttgataatat tgccggccgt
     2341 cgtgtagacg acctccactt tgcccgaaaa tctgcccttc gaataacgtc ctgcaagtgc
     2401 ggcgccaaag cgtatcgcct tctccggaac gtctttcccg gccttcttaa gtataacatg
     2461 agccccgggt atcccgcggg catgaaacca gaagtcttct ttagaagcgg cctttgttat
     2521 ttcgtcgttc tgtgtgttgt tctttccgac caggtacttg aattcgtgcc tctcaaagat
     2581 cctcggcccg gaaggaggtg tcctctttct cttcttttcg acgctctcgg ccctgatgat
     2641 tccggcctgt ctcaattcgt ctttgaagga gtccagctcg accagattct ccgaaagact
     2701 taccatctct tcgagctgtt cgagatactc cctctcgccg ttgagttctt tcagtctcct
     2761 ggttacctga atctctttcc tgcgggattt gtcgtagtat ttgaagaaaa gctgggcgtt
     2821 ctccgaaggg gtgagccgat cgtccagatc tatcctgatc ctttcaccgc tctcccagtc
     2881 ttcgagttcg atactggggg ttctatcttt cagtctgtaa aggttggcca ccagcaattc
     2941 tccgtaatac ctgtatctgt cgtaatcgtt aacctcctgc aattccctct cgagcttttc
     3001 gatcaacttt tcctgtctcg cgatcgcttt gagtagcttc cctacaatcg atctacgtct
     3061 gcgatctatc tccttatcga ccccaaaatg gacgagcgcc tcgtttatca ccatagaggc
     3121 ctgcatggct ttgcaatcgc ccttgaaatc cagcggtatg ggcgagatct ccatctctcc
     3181 ctcattcagg caagtgtaaa ggatagaact gttgaaatcg ctggctatct tgccaagaaa
     3241 ggtacacagt tcatctagct tctcatcttc cagctcgtcc ggggcgacct cctccagacc
     3301 caggtaaccg gttatattat gtgaggtcct tttcgagata ccggagaatg tgctccatat
     3361 agctttgggg agtttctcac ccgatacgag catcgaactc ctgatctccc cgtccgacag
     3421 gctcgacaga tcctttcccc tcggttcggg cggcacgtac ctaaccccgc gggcgagggt
     3481 cctcttttcg gttatcatct cgcgaaaggc gtgggtgacg gttcccttac catcgagcac
     3541 aatcatattc gaaccaggtc ccatcaactc gatgaccaat tgatagatct ctctcatacc
     3601 gaaggcgttt ccgccctcca ggattaaacg gacgatcctg tccgtcttct cctgggtgat
     3661 cgactcgaga aagagtccgt tcaaatgcct gcgaagataa atactgaagg gcgtctccag
     3721 agaagagctt tcatccttct cgccaaaaga gatataggga gtcgaaggat cgagagagac
     3781 gttcaatccg gaccgggaga atttgaagaa gaactcgctc ttacccttct ggtaaatctg
     3841 acgtagctgc tgccctacta tgttttgcag tccgttgacc accctcttca aaaccagacc
     3901 gtcgaatatc acagatataa cacctcattt ctgaattcta actatagagc gaaagagccg
     3961 tccttggccc ttcgtccagg agcatgaacc cgtcctttga aagagcgtta ggagcggttc
     4021 tccgtttcga cgctgcgcgt ccaggttcct ggtaaaacag ttcacactcc acttctcaac
     4081 cctcaactca ccacccacta cctactctac ccaccatggc tcctgttgcc cttcatacct
     4141 cacccatctg tggaagtgat gaacctgtat tcacccttcg tcccttcgaa cagagagaag
     4201 agggtagatc ccgatcccgt cattctggtt gcgatggcct ctcttttctc tttgaattta
     4261 tcgtaagctc tcccgatctc cggaaacatt gcgaaaactg gcccctcgaa atcgttcttc
     4321 gagagcctgc caattagtgt caaatcccgc tcccttaaag ccgaaagaag cttttctacc
     4381 tctttattcc ctaactcgtg tccgtctgcc gatctgccca gaagggtgta agccagaggg
     4441 gtcgagaccc cgatccccgg gaagcagaga tctaccgagt agtcttccag atcgccggga
     4501 aaggtcagaa cttccccccg gccggtcccg attgccgtcc cgccccttag aaaaaagggt
     4561 acatcgctcc cgatagagca ggccacttcg aaaagcgctt ctctttcgat gtgaaaggtc
     4621 tctccgaggt atctcagaag cgccgcagcg tctgagctac cgcctccgag acccccgccg
     4681 gcaggtatct tcttttttag ctccattccc agagcgagct ctttgccaag gtgcttctcc
     4741 acggctttaa cagctttata aagcgtgttc gaaccattcc acgcaagatc gatgttgctc
     4801 cggaagtagc ccgtgggtac ccccggatgc aggatcaatt cgtcgtagag atctatcgtc
     4861 tggaagatag tcaatatctc gtggtaaccg tcctctctct tttggcctac ctcgagaaag
     4921 agattgatct tcgcgggaca tttcagggtt atttcaaccg gctcattacg atctgcgacc
     4981 atgtttccat cttctctctg tacatttcta gctcttcgaa ttctaccatc gaaccggtga
     5041 acttgtcctt ttcgttgaag ccgtgaaaga ctacgctggc gatatagact atccccatgt
     5101 ggactctgct cacggccgtg gagagatcgt tgaggatacc cagatactcc agcgaggaga
     5161 tatctacctt cacttcctcg tcgatttccc gcctcatgcc tctctcgaag gcctgccaga
     5221 gattccggcc gtcttcgggg tttatatggc cgccgacccc gatcgagtat ttatcgtgaa
     5281 gtctcgactc gccctgcctg ttcgtcctgc gcatcaggag atatctcttt tcgtccctca
     5341 aaatcacgta aggtattatc tgccggccgc tctcgtcgct ttcggcttcc tcgcggggga
     5401 cgaaacgtcc gtaaagctcg acgggttttt ttatacactc taaaggaaga gccagcaacc
     5461 cgttacgggt tgccagctct ccaagacgat caacatcgac tatgagaact ttttccttca
     5521 aaccgttctc cccactacca gctcttgttg gggaagagaa ccatggccga gcccttcagt
     5581 gtcgtgaaaa ttacgtcttc ctcgttgtac tgggaagaag gagccgtctt ctgtaccacg
     5641 ataactccga agtagtaatt ttcatcccag agacggctgc cgggcggcaa tagattgcct
     5701 tcgctgaagg cgaaactcac gaatgtgtcg atatcttcct cgacgaactc ctccactgct
     5761 ccgggtacgt agtaactgtt tatcttgacc aggtgtgtgg cccggttgtc gctttttctg
     5821 gccctgaaga gccacaggtc gtcggtgaag cgatcgatgt tctggacctt gactttccag
     5881 cccgttgcgc gccagccgct gaaagtggtg ggttctattc tgatagccca gtcacccgtg
     5941 gtgggagcgt tgttgaacat ggtgctcatc tgggcgcttg tgtaggtgtt gctgggcagt
     6001 ttcgtccagg cctggaagaa gacctcgccg gcgttgtata tcgccccggc gctcgtcgga
     6061 tcgattccga agagcaatct ctgggcgtag ggatcgagtc ccacggccga ttccaggaag
     6121 ccctctttgt agcggacgaa attggggacc ctgttggacc aggagaagga gaaggcgccg
     6181 aaccagctgg atggctgtcc gaaggtcgtt cctgccttct cgaagacctc caccagaacg
     6241 gatgtcttcg ggttgtaggt gaccgtctgg ctgtattcaa cagtcacgag agcgccatcg
     6301 tcgtcctggc ccgttgccct ggccgttatc ttaactcttc ccgtctcggg cacggtcgga
     6361 atatagacca ctactgtgtc gtctttgaat cgggtgcttt tcatgatctt gagattctga
     6421 gcggtgatgt cctgccagcc gcctccaccg agttccttct cgagtttcca gatataacct
     6481 gccgcgtttg gggcgaatct gaagttcatc gcctctacca ttatcggttt gcccggttcc
     6541 ctgttgcttc cctggatggt caacgatatc gatggaagca tcggccagca agaacttacg
     6601 agaagcccta tggcggcgat gaaggaaatc accagtaacg ttctgaatct tttcattcat
     6661 atcccctcca ttctaagaaa atctttcacg aatccatctt ccttctcgat ttcaagcccg
     6721 tccagtctag cacagtacag ggcatcgagt actttcttta tttccggtcc ctctttcaga
     6781 ccgtacgccc tcttcagacg ctcgccgtcg accgccagct tcgtctcttt gagcctttta
     6841 aggtactcca cgaaagacct gtctctttca tcctcaagct ggctgttgag aaagttcaac
     6901 gtctctgcct ttttcgactt gatcgcacag tgatattgcg aaggtttttc cgggttctca
     6961 cgggagatac tctcggcgtt ctccctggca tctctgagcc tctccagaaa gtttctgggg
     7021 agaccgtaac gttgaatgtt ccactgaacc tgctcattaa tactatacct caacagtatc
     7081 atcattagtg tataaagtat tctatcctcc ccgatatagc cgttgtttct cggccgcctc
     7141 agaaaatact tctcgatcgc gaaatcgatc cgcctgtcga agaccactcc cgggaagagc
     7201 ttctctataa cgcccagtgt cgaaagcctc cttatagctt taaggggctg aggctccagc
     7261 aggattttgt ttagctcgtc cctcaaccgc tggcctgcta ctttgtccag atagtcctcg
     7321 cgaacgcagt ttttaaggag ctccgccgtt cttacttcta tttcgaaacc gaacctctgc
     7381 tcgaatctta cggccctcag aaccctcgtc ggatcctcta tgaagctcag cggatgaagc
     7441 gccctgatgg ttcccctggc cagatcgttt ctcgaaccga agaagtcgat cagcattccg
     7501 aactcctcct ggttgagctt tacggccatg gcgtttatcg agaagtcccg cctgtaaaga
     7561 tccttcttga tggtgctcat atcgacctca ggcagtgcgg ccggtctctt atagaactcg
     7621 gtgcgcgcag tggcgacatc tatcctcagc ccgtcgtgaa agaacatcga actggccagg
     7681 aattccttgt gttccactat ctttatatcg aagtactttt tgaaggccgc cgcgaaactg
     7741 ttcgcatttc cttcgaccac tatgtcgaga tcgaggttcg tcttgttcag cagaaggtct
     7801 ctcacgaatc ctcccacgat gtaagtcggc atacccagct cgcttccaac ggcgcccagg
     7861 aatctgagca tggtcagtag cctgccttcg acgcgctctt ccagtagccg tgctatcgaa
     7921 agcttgacat agttctggtc ttccttctgc tctctgctct gaaggaacct ctggcgactg
     7981 aaagggctct tcaaaagatc cgttctggtc actatgcctg tgagaatacc gttttcaagg
     8041 accagcgccg aagtagtatc gttttccacc attctgtccg taaccgactg aacggaatcg
     8101 tttgccgaag ccacaacgaa gaaggggtcc atgatttcaa cgatcttcct gtcgccaagg
     8161 ttgtgcctgg aggccttctc cacgttcctg tgtctggcga tacccacgat cttgtcctga
     8221 tcgaccaccg gcagagtgtg gtggcccgtt ctctgcatta ttttcagaac gtcctcgact
     8281 ctcatttccg tcagaacggt cctgaccgga gaggacatta tgtccctgac ctttataagt
     8341 ctctctatat tcttctcgaa ggctcccctg atggccagat ctgccttttc gaagtcgccc
     8401 tcttcgaaat aacccatcgt gaaggcccct acgccgttcc ttaaacttcc gccgagcccg
     8461 ttgaggtcga ggttcatatg cctcgatctg atagtgaaat aaatcctctc tccaaccttc
     8521 accagtacca ccagattctc cacgcccaga aaagaccaga gtttctctat cgccagactc
     8581 ataccgctgg ggagagagtc tctctgtgct ctggcgaagc taatctctac ccccccgaca
     8641 tcgaccgtct cgatgttgag cagaagcgtc tcgaccagct tttgctgcga tagactcaac
     8701 ctgtagctgg aatagacacc aagttcgtcg ggatggacgc ccgaatccag cagccaggcg
     8761 gccgttctca aatcctgcgg ttttgtgttg gcgtgcgtga agttgccggt ctccctgtag
     8821 atcgccgtgg cgaagagcga tgcctcgttc tgcgtgatgt gcttcttctt ctttctcagg
     8881 agggtgcaca taagggtggt gcaggctccc agatcgcggt aggtaccagg cttctcgccg
     8941 gtggcctgaa gatgatgatc gaagaatttg acttccgtgc cggcggttat cagccgcctt
     9001 atcgaaaagg gaagcctctg aatatctgaa gtatcgacta ctatgagcga gttcagtctt
     9061 tccagtttca gctccccggt cttgtagaaa gagaggagca gtcctctgga agagagaa
//
LOCUS       DAOOLY010000080         8999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9039_length_8999_cov_5.187165, whole genome shotgun sequence.
ACCESSION   DAOOLY010000080 DAOOLY010000000
VERSION     DAOOLY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9039_length_8999_cov_5.187165"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLD15_05560"
     CDS             <1..465
                     /locus_tag="PLD15_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63404.1"
                     /translation="HINTRPLGLAKGIIAIGSVAVGVISLGGISLGVISLGGISLALS
                     LSLGGVSASLWVAFGGLAVALYMAVGGLAIAYDIAIGGAALAKNYALGGFASARVAIG
                     GEARGIIAVYYQKGLGDYLLRLPATAHQIVGFITSKTEGISGLFEWIIRLLF"
     regulatory      539..639
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     regulatory      664..764
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            816..1316
                     /gene="purE"
                     /locus_tag="PLD15_05565"
     CDS             816..1316
                     /gene="purE"
                     /locus_tag="PLD15_05565"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPB63405.1"
                     /translation="MALVAVVMGSKSDLPVARRCTETLERLSIGFELRIMSAHRTPST
                     VVDYALSAREKEIDVIVAIAGKAAHLAGVIAACTCLPVIGLPVKSSTLDGLDSLLSTV
                     QMPKGVPVATVAIDGAENAALLAARILSIKYPQLRERLESYARQMREEVLKNDEELRS
                     NTEGGN"
     gene            1324..2034
                     /locus_tag="PLD15_05570"
     CDS             1324..2034
                     /locus_tag="PLD15_05570"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB63406.1"
                     /translation="MRDLLYEGKAKKVFLTDRDDRYLIRYKDDATAFNGEKKGSIVGK
                     GAINNTMSAYFFQLLQSEGVESHFIEQTGPAEMVVRKVEIIPLEVIVRNIAAGSMSAK
                     LGIAEGTQLARPVVEFSYKRDDLGDPLVNDDHILALGLADGEELTKLRETALKVNTIL
                     REYLSKAGITLVDFKLEFGRLDNGEILLADEISPDTCRFWDSETGEKMDKDRFRRDLG
                     NIEEGYRKALKRVLGGGK"
     gene            2031..2276
                     /gene="purS"
                     /locus_tag="PLD15_05575"
     CDS             2031..2276
                     /gene="purS"
                     /locus_tag="PLD15_05575"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPB63407.1"
                     /translation="MKATVYVTLKNGIADPQGLAVRNALDSMGFDGVKRVRIGKIIEI
                     ELEDGCLDETKVEEMCRSLLANTVMEDFTFEMSGEKR"
     gene            2273..2989
                     /gene="purQ"
                     /locus_tag="PLD15_05580"
     CDS             2273..2989
                     /gene="purQ"
                     /locus_tag="PLD15_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPB63408.1"
                     /translation="MKVAIVVFPGSNCDLDAYHAVIEAGEEASYVWHMEKSLEGFDAV
                     ILPGGFSYGDYLRCGAIARFSPIMASVREMAESGRPVLGICNGFQILTEAGLLPGVLL
                     RNRDLKFICKNVFLRVESTASPFTKALESRSVLKIPVAHGDGNYFVDGETLWSLEENG
                     QVIFRYCSADGEVAPQFNPNGSVSNIAGVSNARGNVLGMMPHPERVCEEITGGTDGLL
                     LFKSMISFLKGGMAGELVNS"
     gene            2970..5174
                     /gene="purL"
                     /locus_tag="PLD15_05585"
     CDS             2970..5174
                     /gene="purL"
                     /locus_tag="PLD15_05585"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPB63409.1"
                     /translation="MSSSTLDYSLAGLSQSEFDLICEKLCREPNLLELAMFGVMWSEH
                     CSYKNSRALLKTLPTGGNAILQGPGENAGIVDIGEGLAVVMKVESHNHPSAVEPYQGA
                     ATGVGGIIRDIFTMGARPIALLNSLRFGELDKSRTRYLFENVVKGIGDYGNCMGIPTV
                     AGEIYFDDHYAGNPLVNAMCVGIMRHEEVKYARAKGVGNPVMLVGATTGRDGMGGAAF
                     ASVELTEQSLERKSAVQVGDPFMEKLLLEACIELFKTDAVIAIQDLGAAGLTSSCCEM
                     ASRGRSGIKIELSCVPRREEAMTPVEIMLSESQERMLVVVKRGREKEVGSIFEKWGLS
                     GTVIGEVTDDGLFTVLERGKVVASLPARLLADDAPVYTRESTRPAYLESVGLFDLHER
                     EDMNIKETLIALLARPTIAGKEWVYSQYDHMVRTSTVTVPGSDSAVLRIRGTDRAIAL
                     TIDGNGSYCYLDPFRGAAIAVAEAARNLVCSGARPLAITDGLNFGNPEKREIYWQFKE
                     TIAGLAAACNVLGTPVISGNVSFYNETETGAIFPTPIVGMVGVIEDISKVTESFFINE
                     GDAILLLGENTHELGGSEYLRMLTGKTIGECPAIDLEREKKVQRLCLEAIERRLLNSA
                     HDCSEGGLAVALSECCFGKGLGAKIELNDTLPIDALLFGEAQSRIVVSLDESKLGSLL
                     RLAGEFGVPVQVLGRVDRSELKIDVCGREVLSVPVGELEARWKGAIECAMRS"
     gene            5156..6577
                     /gene="purF"
                     /locus_tag="PLD15_05590"
     CDS             5156..6577
                     /gene="purF"
                     /locus_tag="PLD15_05590"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPB63410.1"
                     /translation="MRDEIVREELKEECGVFGLFSREGRASRTTYYGLFALQHRGQES
                     AGIAISEGEDIRLHKAMGLVGEVFDDARLEELKGDLAVGHVRYSTSGDSSVINAQPLV
                     FKTKHGAIALCHNGNLANSLALRESLKERGSIFQTTVDTEVIAALLAGSPDEDLVVSI
                     RNVMKELKGAYALIIMTCDRLIGVRDPLGIRPLCIGRKGNDLMLSSESCAFETVGGNL
                     MRDVLPGEIVVIDRSGMKGYLPEEKNEEKLCIFEFIYFGRPDSIMKGQSLYMSRKRAG
                     KILYREFPAEADFVCSVPDSGTPSAIGFSEASGIPFLEGLVKNRYVGRTFIKPSQSQR
                     EIGVALKLNVLEEVVRGKSIVLVDDSIVRGTTIKKLISRLRDCGAGEIHLRISAPPVM
                     WPCFFGIDTPSRDELLGARFSVEEIGRLVGADSIGYLSLEGLVEATSRSGFCTGCFSG
                     QYPVDIEGADGRYTLDTDQKVRT"
     gene            6574..7599
                     /gene="purM"
                     /locus_tag="PLD15_05595"
     CDS             6574..7599
                     /gene="purM"
                     /locus_tag="PLD15_05595"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPB63411.1"
                     /translation="MSDYRSSGVDIDAGDRAVELMKRHVETTRRPGVLGGIGSFGGLF
                     RPDFDGMKEPVLVSGTDGVGTKLKLAFAMDIHDSVGIDCVAMCVNDIISMGATPLFFL
                     DYIAIGRLEPVKIEKIVSGVCEGCRQASSALIGGETAEMPGLYAAGEYDLAGFSVGLV
                     DRQEIIDGSTIENGDILIGWPSSGFHSNGFSLVRKILSDEKINLHETVPGIARPIGLE
                     LLTPTRIYVSLFERLKGLCKPKGIAHITGGGLLENVPRVIPKGLKCEIHTDSWEVPPL
                     IEFIVERGSVETREAYRTFNMGLGLVTVVSPDEVDAVMHGMKDEKPVEIGAVVAGEGG
                     VTLCRRG"
     gene            7584..8237
                     /gene="purN"
                     /locus_tag="PLD15_05600"
     CDS             7584..8237
                     /gene="purN"
                     /locus_tag="PLD15_05600"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPB63412.1"
                     /translation="MSKRIAVLVSGNGSNLERLIELSRAGFIDGEISLVISSNEKAFA
                     LERAKRHSIPSRILHFNSRSCDAYSDVILQTIVDCEIDLIVLAGFLKILSGKIIERFR
                     NRIINLHPSLIPSFCGKGMYGERVHRAVIESGVRITGATVHFVDGGTDTGPIILQEAV
                     PVLCDDTAESLSGRVALVEHRLLPKAVSLFCKDRLTVRGKRVLITGGSYENSVVERL"
     gene            8209..>8999
                     /gene="purH"
                     /locus_tag="PLD15_05605"
     CDS             8209..>8999
                     /gene="purH"
                     /locus_tag="PLD15_05605"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPB63413.1"
                     /translation="MKTALLSVSNKEGIVDFAKQLSDRGFRILSSGGTQRELVASGVE
                     VFAVSDLTGFPECLDGRVKTLHPAVHAGILARRELPAHMEKLAQLGIDTIDLVAVNLY
                     PFKEAISGDGIMLEEAIENIDIGGPAMLRSAAKNYGDVIVLVDPADYTGVLRELDAGG
                     PSRIFRLKLASKVFRHVAHYDSMIASHLSGLCGETLTEKMSVTLEKVQDLRYGENPHQ
                     RAALYRYPLSYPLGGYVQIQGKELSFNNLTDAGAAIELLREFTSDT"
BASE COUNT         2176 a         2143 c         2747 g         1933 t
ORIGIN      
        1 catatcaaca caaggccgct cggcctggcg aaagggataa tagccatcgg gagtgtcgcg
       61 gtgggtgtta tctcgctcgg cggcatctcg ctgggtgtaa tatcgctagg tggtatttcg
      121 ctggcactct ctctatcgct gggcggagtt tcggcgagcc tctgggtagc cttcggtgga
      181 ttggccgtag cgctttacat ggccgtcggc ggcctggcga tcgcttacga catagccata
      241 ggcggagcgg cactggcgaa gaactatgcg ctcggtggtt tcgcttcggc cagagtggcc
      301 ataggcggcg aggcaagggg gataatcgcc gtctattacc agaaaggcct tggagattac
      361 cttctgaggc tgccggccac ggctcaccag atagtgggct tcatcacttc gaaaaccgag
      421 gggatcagcg ggctatttga gtggattata aggcttctct tctgatagga agtgttaaga
      481 gaaaattatc atagaaaaac tccctgttac tcgttttatg atagtatttc agagtaggaa
      541 aactgaataa agcatcgcct gtataatcgt gggaatatgg cctgcgagtc tctaccggca
      601 accgtaaatt gcctggctac aggtgacttt tcgtttttat aaccaaacga gagtcgccta
      661 tggctcttgt ttttgttttt cacctgtata atcgcgggaa tatggcccgc gagtctctac
      721 cggcaaccgc aaattgcctg gctatgggtg agtcctgtga tttaacgacc ccgcgggctc
      781 gcctttgcgg ggtcattcta ttctggaggt tttctatggc tttagtggct gtggtgatgg
      841 ggagtaagtc cgacctcccc gtggcccgaa gatgtactga aacactcgaa agactttcca
      901 tcggttttga attgagaatc atgtccgcac accgcacgcc ttccaccgta gtggactacg
      961 cactctccgc gagagaaaaa gaaatagatg tgatcgtggc catagccggg aaagcggctc
     1021 atctggccgg tgtgatcgcc gcctgcacgt gcctgccggt aatcggtctt ccggtaaaat
     1081 cctccacgct cgatggactg gactcgctgc tatccaccgt ccagatgcct aaaggcgttc
     1141 ctgtggccac ggtggcgatc gatggcgccg agaacgcggc gcttctggcc gccaggatac
     1201 tgtccataaa atacccgcaa ttgagggaaa gactggaatc atacgcgcgg caaatgcggg
     1261 aggaagtttt gaagaacgac gaggagttac gttcgaatac agagggaggg aattgagttg
     1321 aatgtgagag atctgcttta cgagggaaag gccaagaaag tcttcctgac agacagggac
     1381 gacagatacc ttataaggta caaagacgac gcgacggcct tcaacggaga gaagaagggc
     1441 agcatagtcg gtaagggcgc cataaacaac accatgtcgg cctacttttt ccagttgctc
     1501 cagagcgagg gtgtggagag ccatttcata gaacagacgg gccctgccga gatggtagtc
     1561 aggaaggtgg agataatccc gctcgaggtt atcgtaagaa acatcgccgc cggttctatg
     1621 tccgcaaaac tggggattgc agagggaacg caactcgcca ggccggtggt cgagttctcc
     1681 tacaagaggg atgacctggg agacccactg gtcaacgacg accatatcct ggcgctgggc
     1741 ctggcagatg gggaagaact caccaagttg agagagacgg cgctgaaggt taatacgata
     1801 ctgagggagt atttatcgaa agccggaata acactggtag atttcaaact ggaattcggc
     1861 aggctggata acggggagat cctgctggcc gacgaaatca gtcccgatac ttgccggttc
     1921 tgggatagcg agacgggaga gaaaatggat aaggatcgtt tcaggaggga tcttgggaac
     1981 atcgaggaag gttacaggaa ggctttgaaa agagtcctcg gaggtggaaa atgaaggcaa
     2041 ccgtctatgt aactctcaaa aacggcatcg ccgatccaca gggactggcg gtcaggaacg
     2101 cgctcgattc aatgggtttc gacggggtga agagagtgcg gataggcaag atcattgaga
     2161 tagagcttga agatggctgt ctggacgaga caaaagttga agagatgtgt cgttcccttc
     2221 tggccaacac ggtcatggaa gatttcacat tcgaaatgag cggtgagaaa agatgaaggt
     2281 ggccatcgtg gtctttccgg gatccaactg cgatctcgat gcctatcacg ccgttataga
     2341 agccggagaa gaggcgagtt acgtgtggca catggagaag agcctcgaag gtttcgacgc
     2401 ggtcatactt ccaggaggct tttcttatgg cgactatctc aggtgcggtg caatagcccg
     2461 tttctcaccc atcatggcgt ccgtcaggga gatggccgaa agtggaaggc ctgttctggg
     2521 tatttgcaat ggtttccaga tactgaccga agccggtctc ctgccgggag ttctgctcag
     2581 aaacagggat ctcaaattta tctgcaagaa cgtctttttg agggtcgaat cgactgctag
     2641 ccccttcacg aaggctctcg aaagccgaag cgttctcaag ataccggtcg ctcacgggga
     2701 cggaaactac ttcgtcgatg gagaaacgct ctggagttta gaagaaaacg gccaggtgat
     2761 cttccgctac tgctcggctg atggagaagt cgccccgcag ttcaacccca acggatcggt
     2821 ctcgaacatc gccggggttt ccaatgcacg gggaaacgtt ctgggaatga tgccgcaccc
     2881 ggagagagta tgtgaggaga tcaccggagg caccgacgga ctccttctct tcaagtccat
     2941 gatctctttc ctgaaggggg gtatggccgg tgagctcgtc aactcttgat tactcgctgg
     3001 caggtctcag tcaatcggag ttcgacctga tctgcgagaa actctgcaga gagccgaatt
     3061 tacttgagct ggccatgttc ggggtgatgt ggtccgagca ttgcagttac aaaaactcga
     3121 gagcgctcct caagacactt ccgacaggcg gcaacgcgat ccttcagggt ccgggcgaga
     3181 acgccggtat agtcgatatc ggtgagggat tggcggtagt gatgaaagtc gagagtcaca
     3241 accatccctc ggcagtcgag ccctaccagg gggccgcaac cggcgtcggt ggaatcataa
     3301 gggatatctt caccatggga gcaagaccga tcgctctgct aaattctttg aggtttgggg
     3361 aacttgataa gtccaggacc aggtacctat tcgagaacgt ggtgaagggg ataggcgatt
     3421 acggcaactg tatggggata ccgaccgtgg ccggggagat ctacttcgac gatcattacg
     3481 cgggcaatcc cctggtcaac gcgatgtgtg tgggaataat gagacacgaa gaggtaaagt
     3541 acgccagggc aaagggagtt ggcaaccccg tgatgctcgt gggcgccacc acggggcgtg
     3601 acggtatggg cggggcggcc ttcgcctccg tcgagctgac cgaacagtct ctggagagaa
     3661 aatcggccgt tcaggttgga gatcccttca tggagaaact gcttctagag gcctgcatag
     3721 aactcttcaa gaccgatgcc gtgatcgcca tacaggacct cggggcggcc gggttgacct
     3781 cctcctgctg tgagatggcc agccgcggca ggagcggaat aaagatagag ctttcctgtg
     3841 ttccgcgaag agaagaggcc atgacgccgg tagagataat gctttcggag tcccaggaga
     3901 ggatgctggt cgtcgtgaaa cggggaagag agaaggaggt cgggagtatc ttcgagaagt
     3961 gggggctcag cggaacggtg ataggagagg tgacagacga tgggctcttc accgtactgg
     4021 agagagggaa agtcgtcgcc tcgctaccgg cgagactgct ggccgacgat gcacccgtct
     4081 atacgagaga gtcaaccaga ccggcctacc tggaaagtgt cgggcttttc gatctacacg
     4141 aacgtgaaga tatgaatatt aaagagacgc tgatcgcgct cctggcaaga ccgactatag
     4201 ccggcaaaga atgggtttac agtcagtacg accatatggt taggacgagc acggtaacgg
     4261 ttccgggaag cgactcggcc gtactgcgca taaggggtac ggatagagcg atagccctaa
     4321 cgatagacgg caacggttct tactgttatc tcgatccatt caggggcgct gcgatagccg
     4381 tcgctgaagc ggcgagaaac ctggtttgct cgggcgccag accgctggcg ataactgacg
     4441 gcctgaattt cggaaatccc gaaaagagag agatctactg gcaattcaaa gagacgatag
     4501 ccggtctggc cgctgcctgc aacgttctgg ggactcctgt tataagcgga aacgtttcct
     4561 tctacaacga aaccgagacc ggcgcgatat tccccactcc catagttgga atggtgggtg
     4621 tgatagagga catctctaag gtgacagaga gctttttcat caacgagggc gacgcgatac
     4681 tcctccttgg cgagaatact catgaactgg gcggtagtga atacctcaga atgctcacag
     4741 gtaaaactat cggagagtgt ccggcgatag atctcgaaag ggagaagaaa gttcaaaggc
     4801 tctgtcttga agccatagaa aggaggctac tcaactcggc gcatgattgc agcgagggcg
     4861 gactggccgt tgcgctttcc gaatgctgtt ttggaaaagg cctcggggcg aaaatcgaac
     4921 ttaacgatac tttgccgatc gatgcgcttc tcttcgggga ggcccagtcg aggatcgtgg
     4981 tctcgctgga tgagtcgaag ttaggctctc ttctcaggct ggccggagag ttcggcgtgc
     5041 ccgttcaggt cttgggcagg gtcgaccgta gtgagctgaa gatagatgta tgcggcaggg
     5101 aggtcttgtc tgtgccggtc ggggagcttg aagctcgctg gaagggggcg atcgaatgcg
     5161 cgatgagatc gtgagagagg aattaaagga agaatgcggc gttttcggct tattctcgag
     5221 agagggaagg gcctccagga cgacctacta cggtctcttc gctctccagc acagggggca
     5281 ggagagtgct ggaatagcca tctcggaagg cgaggacata cgcctgcaca aggccatggg
     5341 tctggtcggg gaggtcttcg acgatgcgag gctggaggaa ttgaagggag atctcgcggt
     5401 cggtcatgtc cgctactcaa cctcgggaga cagcagtgtg atcaacgcgc agccgctagt
     5461 tttcaagaca aagcacgggg cgattgccct ctgtcataac ggtaatctgg ccaactctct
     5521 cgcacttcgt gaaagtctca aagagcgtgg atctatcttc cagacgacgg tagatacgga
     5581 ggtcatcgcg gcgctcctgg caggtagtcc cgacgaagat ctcgtggtct ctatacggaa
     5641 tgtgatgaag gaattgaagg gcgcttacgc tctcataata atgacctgcg acagattgat
     5701 cggcgttaga gatcccttag gtataagacc tttgtgtata ggaaggaaag gaaacgacct
     5761 gatgctctcc tctgagagct gcgccttcga aacggttgga gggaacctta tgagggacgt
     5821 gttgccaggc gaaatcgtcg tgatagacag gtcgggaatg aagggctatc ttcccgaaga
     5881 gaagaacgaa gagaagcttt gcatcttcga gttcatatat ttcggcaggc ccgacagcat
     5941 aatgaagggc cagagtctat atatgagtcg aaaaagggcc gggaagattc tgtacagaga
     6001 gtttccggcc gaagccgact tcgtctgttc cgttcccgat tccggtacgc cttcggccat
     6061 aggcttctcc gaggcctcgg gaataccctt tctcgagggg ctggtgaaga ataggtacgt
     6121 gggccgaacg ttcataaagc cttctcaaag ccagcgagaa atcggagtgg cgcttaaatt
     6181 aaacgtactg gaagaggtcg tcagagggaa atcgatcgtt ctcgttgacg attctatcgt
     6241 caggggaact accataaaga agcttatctc cagactcagg gactgtgggg cgggagagat
     6301 acatctgcgg atcagcgcgc cgcccgttat gtggccctgc tttttcggaa tagacacccc
     6361 ctccagggac gagctcctcg gggcgcgttt ctctgtggag gagatcggac ggctggttgg
     6421 agccgacagc atcggttacc tcagcctgga gggactagtc gaggccacct cccgatcggg
     6481 cttctgcacg ggctgcttct cgggacagta cccggtggat atagaaggcg ccgacggaag
     6541 gtacacgctg gataccgatc agaaggtgag aacatgagcg attacaggag ttccggcgtc
     6601 gatattgacg ccggtgacag ggccgtagag ctcatgaagc gacacgtgga gactaccaga
     6661 aggccagggg ttctgggagg cataggctcc tttggaggac tctttcgtcc ggattttgac
     6721 ggaatgaagg aacctgttct ggtctcggga actgatggtg tcggcaccaa attgaaactt
     6781 gccttcgcga tggatattca cgatagcgtt ggaatagatt gcgtggctat gtgcgtcaac
     6841 gatataattt ccatgggcgc gacaccgctc ttctttctag attatatagc gatcggaagg
     6901 cttgagccag ttaagatcga gaagatcgtg agcggcgtat gcgaaggatg ccgacaggcc
     6961 tccagcgccc ttatcggagg agagactgcc gagatgcccg ggctgtacgc agcaggcgag
     7021 tacgacctgg ccggtttttc cgtggggctg gtcgatagac aggagataat cgacggctcg
     7081 acgatcgaga atggagacat cctcataggc tggccctcat caggctttca ctccaacggc
     7141 ttttcactcg ttcgaaagat cctctcggat gaaaagataa atctgcacga gaccgtcccc
     7201 ggcatcgcga ggcctatcgg tctcgaattg ctcaccccca ccaggatata tgtctcgctc
     7261 tttgaaagat tgaaagggct ctgtaagccc aaaggaatcg cacacatcac cggcggggga
     7321 cttctagaga acgtaccgag ggtgataccc aaaggtttga agtgcgagat acacaccgac
     7381 tcctgggagg tgcctccttt gatagagttc attgtcgaga ggggcagcgt cgaaaccagg
     7441 gaagcctaca ggaccttcaa catggggctc ggtcttgtca ccgtggtctc tcccgacgaa
     7501 gtagatgccg tcatgcacgg aatgaaggat gaaaaaccgg tagagatcgg tgctgtcgtg
     7561 gccggggagg gcggtgttac actttgtcga agaggatagc cgtcctggta tcgggtaacg
     7621 ggagcaacct tgaaagattg atcgaactct ccagggccgg cttcatagat ggagagatct
     7681 cgctggtaat ctctagcaac gaaaaggcct tcgcgcttga gagagcaaag agacactcta
     7741 taccctcgcg catattgcat tttaacagtc gatcctgtga cgcctacagc gatgtgattt
     7801 tacagaccat cgtagattgc gaaatagatc tgatcgtcct cgcaggattt ctaaagatcc
     7861 tatctggaaa gatcatagag cgtttcagga acaggataat aaatctgcac ccttctctga
     7921 taccctcttt ctgcgggaag gggatgtacg gggagagggt tcacagagcg gttatcgaga
     7981 gcggagtcag gataaccggc gcaacggttc acttcgtcga cggaggaacc gacaccggtc
     8041 cgataatact gcaggaagcc gttcccgtcc tctgcgacga tacggccgaa agcctctccg
     8101 gaagggtggc tctggtcgag cacagattgc tgccgaaggc cgtctctctc ttctgcaagg
     8161 atagattgac cgtcagggga aagagagttt tgataacagg aggttcgtat gaaaacagcg
     8221 ttgttgagcg tctctaacaa agaagggata gtcgatttcg cgaaacagct ttcagataga
     8281 ggtttccgga ttctctccag cggaggcaca cagagggagc tggtcgcttc tggggtagag
     8341 gtttttgccg tatcggatct gaccggtttt ccggaatgcc ttgacggacg tgtgaagacg
     8401 ctccatccgg ccgttcacgc cggaatactc gccagaaggg agttgcctgc acacatggag
     8461 aagctggcgc aactggggat cgatacgata gatctcgtag ccgtgaacct ttaccccttc
     8521 aaagaggcga tctccggcga cggcatcatg ctggaggagg cgatagagaa tatagatata
     8581 ggaggtccgg ccatgctccg ttcggccgcg aagaattacg gcgatgtgat tgtgctggtc
     8641 gatccagccg actacacagg tgttttaagg gaactggacg ccggcggtcc ttccagaatc
     8701 ttcagactca aactggcctc aaaagtcttc aggcacgtgg cccattacga ttctatgatc
     8761 gcctcgcatc tgagcggatt gtgcggcgag actctgactg agaagatgag cgttacgctc
     8821 gaaaaggtcc aggacctcag gtacggtgaa aatccgcacc agagggcggc gctttaccgc
     8881 tatcccttga gttacccgct cggagggtac gttcagatac agggaaagga gctctccttc
     8941 aacaacctca ccgacgcggg agccgcgatt gaactgctga gagagtttac cagcgatac
//
LOCUS       DAOOLY010000081         8815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9311_length_8815_cov_4.063584, whole genome shotgun sequence.
ACCESSION   DAOOLY010000081 DAOOLY010000000
VERSION     DAOOLY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9311_length_8815_cov_4.063584"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLD15_05610"
     CDS             <1..578
                     /locus_tag="PLD15_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB63414.1"
                     /translation="PMEELVLAMDYIVRNGLAFYWGTSEWSAGELEEAHRVAEKLNAI
                     HPVVEQPQYNMLSRERVEKEYLPIYEKYGMGLTVWSPLASGLLTGKYNDGVPKDSRLA
                     RFPGLKKHLEENGMLSRKNLEKVGKLANVAKELETGLPQLALAWVLKNENVSSVILGV
                     SRVEQLEENLKALEVKEKLTDEVMKKIESII"
     gene            694..1290
                     /locus_tag="PLD15_05615"
     CDS             694..1290
                     /locus_tag="PLD15_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63415.1"
                     /translation="MKRWFFIPAILSMVLALSGCLYIMPVPIPITESLIEPIVFQLPG
                     TPFPINSFAFGSQSIPTLDEIIAEAQKSSPIQIPDNFQITSVQVKARVLWESEDGQLD
                     LKFYISRDPFTDIPLLITDPESIEDNELFNDTILPGENIFERDSGSTPAFSMIIDILN
                     SGVSEDINWLALYDYSATQDSTMTIYVSGTVWVKRFVE"
     gene            1754..2248
                     /locus_tag="PLD15_05620"
     CDS             1754..2248
                     /locus_tag="PLD15_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPB63416.1"
                     /translation="MALQFLSVYVIGFLIWLGFTGSFDPLELLLGLVITAIVAAIFSR
                     YSNFRFGLDFPLRVFKFIFRFLPVFIVEMVKANIDVARRVLNPDLPINPGMFRIDTDL
                     KGDFAKFCLANSITLTPGTLTVDMDEKGLLIHSIDLKEDLQSTEATGLIPESFEKRIK
                     EVFE"
     gene            2245..2499
                     /locus_tag="PLD15_05625"
     CDS             2245..2499
                     /locus_tag="PLD15_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPB63417.1"
                     /translation="MIAFSIFFSLILLGAFLTLYRLFKGPTTPDRVAALDILNVMLTG
                     AIVLFSLIDRNSLFLDIALVYAALSFLETVVVIRYLEGRK"
     gene            2496..2840
                     /gene="mnhG"
                     /locus_tag="PLD15_05630"
     CDS             2496..2840
                     /gene="mnhG"
                     /locus_tag="PLD15_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPB63418.1"
                     /translation="MIVLDILGYIMISIGAFFYFLGGLGMFRMPDVYNRLQAGTKATT
                     LGSFSLILGVGLANPAWLMKAIIIVTFIAITNPVGSSVIARAAMKKGIEPVGRKELVE
                     VDQRPSEGGEDE"
     gene            2837..3079
                     /locus_tag="PLD15_05635"
     CDS             2837..3079
                     /locus_tag="PLD15_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPB63419.1"
                     /translation="MNVFSLITGVAIVVLALVAIEARRLITSVIMLSAVGLLSVVLFV
                     IMKAPDVAITEASVGAGLTTAIFLLSLRKLKRDDIE"
     gene            3076..3834
                     /locus_tag="PLD15_05640"
     CDS             3076..3834
                     /locus_tag="PLD15_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HPB63420.1"
                     /translation="MKRIIAGIIALLFFFTMSTVLNTDSQTVSGIPRYGEFDLSERNS
                     HSYVTKSVNDTLKEVKFGSTTDAETGSANFVTSIVVNYRSFDTMGEVTVLFLSATGVG
                     LLLGGDKKRLLSPIKASPIVKTAAKIVSPLLLILGVYVFTHGHLTPGGGFPGGAIIAA
                     AFLLLVMVDDERRAPAMLKVLEGSMGLAYVLLGIGGLLISGSFLQNFLPTGVVGNLFS
                     AGLIPIVYSLIGLKVGAELSGIVDDFVSEGGDNI"
     gene            3831..4181
                     /locus_tag="PLD15_05645"
     CDS             3831..4181
                     /locus_tag="PLD15_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HPB63421.1"
                     /translation="MIQYFSLLLTAIGIYGLLSQKNLVKLIVSLNIAEIGVNLFIVSI
                     GYIEKGVVPILSSENNSSTLLFVDPLPQALVLTSIVIGVGITALALAIIVTISHSKGT
                     IDIDELTERGGAEE"
     gene            4178..5620
                     /locus_tag="PLD15_05650"
     CDS             4178..5620
                     /locus_tag="PLD15_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPB63422.1"
                     /translation="MNPVWLIAVPLALAFISTFANKISNWLLILSAIFNAVAGIFLSF
                     SSGTVSYSIGGWRPPFGIDLIVTDATRILLPVANILFFFAVIIHFSKKRASEKFSVVY
                     TISLASLNGILLTNDLFNLFVFLEIAGVAAYLLAMTGKKGESKVAAFKYLLLGSVGSL
                     LYLLGVAVLYAAAGTLNISAIATKVSDGLLNGDVMLVSSLLMIAGLGVESKLVPFNGW
                     VPGVFTKSSNMATLVLASVYPVAMISTFSRVILAVGDRRALDLVIVLGVATVLIGEVI
                     AFKQRNLRSTLAYSSIAQSGLAIFLVGIGTVEALTASVMLIINNALAKFVLFSVDSYT
                     TEELGADDLESLYGLGRMAPVVGLIFTVSALSISGMPLFFGFRAKLLAISSSFESLLV
                     VPIVILLAAVIEITYYFRWIFALYRPYQGEERARLSLPLELMAIGVVLAAILVFLGSS
                     SYETLMTFSKAGNAMVDAIITLGKNLIGGV"
     gene            5621..7453
                     /locus_tag="PLD15_05655"
     CDS             5621..7453
                     /locus_tag="PLD15_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPB63423.1"
                     /translation="MSILTLLVASLLAAPILYLLAKLNTKIAYGAYAALQVFSFIYVF
                     FTGRSNVSYEVIGITERLNFNFGITPTNWFFASIVLLIISMSAIFMFPLKKSPVKIFL
                     LALVSAGTLGAVFSQDFLTLMIFWEISTWASLILIIQDKEKSPREAIKYVTIASIGTY
                     SMLFAIFYMLDKLGSIDFSTVATRIESATVEVQLVILISLGVMVLAKMGVFPLHIWLR
                     GSHSSAPDEFSPILSGALTKIGAFLLFTITAALPSFRIFSQLPVFNGIPMVNYTFALL
                     GGISIVVGTVMAIRMEDAKELIAFSTVSNSGYIVMALSIGGTYATAGGLMHVLNHAMA
                     SAAMFMAIAAVAYRTGTTKMHEMGGLILKMPVTFATYLVAIISVAGIPPTSGFVSKWL
                     IYQQLVQNGLPFLAFAAFFGSIGSFMYVFKPLAGIFLGQLKPQHRNLKEVPLVMVVPM
                     AILTLLTVFWGMLPSNAIKTINAVTASVGINAVEVTFSRIVALTGEWDSILVTTVFAI
                     GFFISLFIFMRAKKARQVDLMDNYTAGDFLYTSELYHFSYRMYRPFDRLFEKWPSMED
                     WLSSLSDKIKELGALLKAIFYPANPQLYIGLAMVAIIGTFWWLR"
     gene            7459..8361
                     /locus_tag="PLD15_05660"
     CDS             7459..8361
                     /locus_tag="PLD15_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPB63424.1"
                     /translation="MIITILSAIALLILAFAYTVTLEGIARKIEARIHRRCGPPFWQN
                     FIDIFKSLTKHSISHGFIFDFGVLMALGGTIATVLFIPAGNLVVFPGLDNVFVIIYLL
                     AIGLLGMAMSAVGSGNPNASIGIGRALTQMLGYELPFMIVLLGVFFHFRTSSLSELAV
                     LQNSGGTYNVLIMPIGAVVGFVSLMGMLGKKPFDTFIAPAEIASGPLVEYSGKYLGLL
                     MTQHAFGTFIEIGLFVNLFLGGGKTIWEFLLKFFLVYFLAVIISSIMPRFRVEQAVKF
                     YWKWPLILSFIQVVIVVFIMGRRV"
     gene            8361..>8815
                     /gene="nuoB"
                     /locus_tag="PLD15_05665"
     CDS             8361..>8815
                     /gene="nuoB"
                     /locus_tag="PLD15_05665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPB63425.1"
                     /translation="MNNEQTAWERVANMLRARSLWMLYYCTGCGAIELPPTMTARFDM
                     ERFGIGPMATPRQADILLVTGYLSTKTLRRVIYSYEQMQAPKYIVGFGSCTINGGIYY
                     DSYATINRLDLYVPVDLYLAGCMPRPEAIVNGFKNLMDKIDQGEATGWK"
BASE COUNT         2135 a         2015 c         2166 g         2499 t
ORIGIN      
        1 cccccatgga ggagctagtt ctggcgatgg attatatagt cagaaatggg ctggccttct
       61 actggggaac ttcggagtgg agtgccggcg aactcgaaga ggcgcaccgt gtggcggaaa
      121 agctcaacgc aattcatccc gtggtagaac agccccagta caatatgctc tctcgagaga
      181 gggtggagaa ggaatatctc cccatatacg agaaatacgg gatgggactg accgtctgga
      241 gcccgctggc cagtggattg ctcaccggca aatacaacga tggagttcct aaagacagcc
      301 gcctcgccag attccccggt ttgaagaaac acctggaaga aaacggcatg ctgtccagga
      361 aaaatcttga aaaagtcgga aaactggcca atgtggcgaa agagctggag accggtttgc
      421 ctcaactggc tctggcctgg gttctgaaaa acgagaacgt gagttccgta attctcgggg
      481 tcagcagggt cgagcagctc gaagagaact tgaaagccct cgaagtcaaa gaaaaactga
      541 ccgatgaagt aatgaaaaag atagagagta taatctaaag tacagtcctg aacatctcgc
      601 ccggtgtgct tctcgagtag aaattcctgt tcgatccaca ccgggctttc ttgaagataa
      661 agcggctaaa atttctggag gggagagatt tttatgaaga gatggttttt cattccggcg
      721 atactttcga tggtactggc gttgagcggg tgtctataca taatgccagt tccaataccg
      781 ataacagaga gcctcatcga acccatagtc tttcagctac cgggcacccc tttcccgata
      841 aatagctttg ccttcggcag ccagagcatc ccgactctcg acgagattat cgcagaggct
      901 caaaaaagca gcccgattca aattccagat aacttccaaa tcaccagtgt tcaggtgaag
      961 gccagggtgc tctgggagag cgaagatggc cagctagatc taaagttcta tatttcccgc
     1021 gatcccttca cggatatacc tctactgata accgatccgg aaagcataga agacaacgaa
     1081 cttttcaacg acactatcct tccgggcgag aatatcttcg agagggattc cggttccact
     1141 ccggcatttt cgatgataat cgacatactg aacagcggag tttccgagga cataaactgg
     1201 ctggccctgt acgattacag cgccacgcag gattcgacaa tgaccatata cgtgagcgga
     1261 accgtctggg tcaagaggtt tgtggaatag attttttgat ttaacgcatt tcacattgaa
     1321 atcctttcca atggactggt gtcatgcact tttgaagtga aaaccagcag tgtttcaacg
     1381 ctcgagcttt agtcttgaga tttatgactt ctcaccgttt cgtataattt tacagtcttt
     1441 ctaaacggct tgcctgatag gtatgttgga ctattatata tatcagatgg cgatttttgt
     1501 cttcaagtaa tggctgaaaa tgcctgcgat ttgtcccggc tcgaaacagt ttggaagaca
     1561 gggccatgaa tcgcatatcg ttttgtacag caattccgat acgagaccgt attgaaatta
     1621 gtgaatattc gcgagttgag ttttggaact atcgatattc aatactgaga acgctttacc
     1681 catacagggt tatggtatta taaacagtat aaactcaatg catttcacaa atttcattat
     1741 aaggggtggt ggtatggccc tgcagttttt atcggtttac gtaattggat ttttgatctg
     1801 gctcggtttt acagggagtt tcgatccatt ggagttgctc ctgggattgg ttataacagc
     1861 catcgtggcg gctatcttca gcaggtattc gaattttcgt ttcgggctgg actttccgct
     1921 gcgtgttttc aaattcatat ttcgctttct gccggtattc atagttgaaa tggtaaaggc
     1981 aaatatcgat gttgcccgca gggtactaaa cccagatctt ccgatcaatc caggtatgtt
     2041 caggatagat acagacctga agggagactt cgccaaattc tgtctggcca attctattac
     2101 cctgactcca ggaacgctga ccgtggatat ggacgaaaag ggacttttaa ttcattccat
     2161 cgatttgaag gaagatctac agtcaacaga agcaacggga ttaattcccg aaagctttga
     2221 aaaacggata aaggaggttt tcgaatgatt gccttctcca tctttttttc cctgatactg
     2281 ctcggcgctt tccttactct ttacagactt ttcaaaggtc cgacgactcc agacagggtg
     2341 gccgctcttg atatcttgaa tgtaatgttg acgggcgcga tagttctctt ttcgctcatc
     2401 gatagaaact cacttttcct cgatatcgct cttgtctatg cggccctttc atttctggag
     2461 accgttgtgg ttatccggta tctggagggg agaaaatgat cgtactggat attctgggat
     2521 acataatgat ctctatcggg gccttcttct actttctggg gggattgggt atgtttagaa
     2581 tgcccgatgt atataacagg cttcaggcag gaacgaaggc tacgacgctc ggttcattct
     2641 ctctcatact cggcgtagga ctggcaaatc ctgcctggct tatgaaagct ataatcatag
     2701 tcactttcat cgcgatcaca aatccggtgg ggagctccgt aatcgccaga gctgccatga
     2761 aaaagggcat agaaccggtc ggtagaaaag aactggtcga agttgaccaa aggccctctg
     2821 aagggggtga agatgaatga acgtcttctc gctaataacc ggagttgcca tagtagtact
     2881 ggctctggtg gcgatcgaag ccaggaggct gataacatct gtcataatgc tgtcggccgt
     2941 gggattgcta tcggtagttc tatttgttat tatgaaggct ccggacgtcg ctataaccga
     3001 ggcttcagtg ggagccggac taactaccgc catctttctt ctatctttga gaaagcttaa
     3061 gagagatgat atcgaatgaa aaggattatt gccgggatca tcgctctcct cttcttcttc
     3121 acgatgagca ctgtactcaa taccgattca cagaccgttt cgggaatacc ccggtacgga
     3181 gagtttgatc tatcggaaag aaactctcat agttacgtta ctaaatctgt aaacgatact
     3241 ttgaaagaag tgaagttcgg ttcaaccacc gatgcggaaa ccggttcggc caacttcgtc
     3301 acttcgatcg tcgttaatta caggtccttc gatacgatgg gcgaagttac ggttttgttc
     3361 ctctcggcaa ccggcgttgg tcttctactg ggtggagaca aaaaacgtct tctaagtcca
     3421 atcaaagcca gccctatcgt aaaaacagcg gctaaaatcg tttctcctct cctgctgata
     3481 ctcggggttt acgttttcac acacggccat ttaactcccg gtggcggatt cccgggcgga
     3541 gcgattatag cagctgcctt tctgcttctt gtgatggtcg atgatgagag gcgtgctccg
     3601 gctatgctaa aagtcctgga aggttctatg ggactagctt atgttctgct ggggattggg
     3661 ggcttgttga tctccggttc gtttctgcag aactttctcc cgaccggggt tgttggaaat
     3721 cttttcagcg ctggtctgat cccaatcgtc tattctctta taggattgaa agttggggca
     3781 gagctttcgg gaattgtgga tgatttcgtt tcggaagggg gcgataatat atgatccagt
     3841 acttctcttt gttgctgacg gccataggta tctacggcct cctttcgcag aagaatcttg
     3901 tgaagctgat agtatctctg aacatcgccg agataggtgt aaatctcttt atagtctcaa
     3961 taggctatat agaaaaaggg gtagtcccta tactgtcatc tgagaacaat tcatcgacgc
     4021 tgctctttgt cgaccctctt cctcaggcac tcgttctgac atccatagtg atcggtgtgg
     4081 gtattactgc tctggcactt gccataatag ttactataag ccattctaag ggaacgatag
     4141 atatcgatga actgacagaa cgaggaggtg ctgaagaatg aatcccgtct ggctcatagc
     4201 cgtaccttta gcactggctt ttatcagcac tttcgcaaat aagatatcaa actggctgct
     4261 tattctatct gctatcttca atgcagtcgc cggcatcttt ctctctttct cttcaggcac
     4321 cgtgagctac tctatcggtg gctggagacc ccctttcggc atcgatctga tcgtgactga
     4381 cgcgacgaga atactccttc ctgtggcaaa cattcttttc tttttcgcag ttatcataca
     4441 cttcagtaag aagagagctt cggaaaagtt ttcggtcgtc tatacgattt cgttagcctc
     4501 gctgaatgga attcttctca caaacgatct cttcaatctc tttgtttttc tcgaaattgc
     4561 cggagtcgcc gcctacctat tagccatgac gggcaagaaa ggagagagta aggtcgccgc
     4621 tttcaagtat ctattgctcg gttcagtagg ttcgctgctg tatcttctcg gagttgccgt
     4681 gctttatgcc gctgcgggga ctctcaacat ctcagcgata gctacaaagg tttcagatgg
     4741 tctgttgaac ggcgatgtta tgctcgtatc ctctttgctt atgatagcgg gcctgggagt
     4801 tgagtcgaag ttggtacctt tcaacggctg ggtaccggga gtttttacga aatcatcgaa
     4861 tatggccact ctggttctcg cttcagttta tcctgtcgcc atgatctcaa ccttcagcag
     4921 ggttatattg gcggtgggtg accggcgtgc tctagatctg gtaatcgttc tgggtgttgc
     4981 gacggttctg atcggagaag tcatcgcctt caaacaaaga aatttgagat caacactcgc
     5041 ctattcgagt attgcccaat cgggtcttgc aatctttctg gtaggtatag gaactgtcga
     5101 ggcgttgacg gcaagcgtta tgttgattat aaacaatgcc ctcgccaaat tcgttctctt
     5161 ctcggtggac agctatacta ccgaagaact gggtgctgat gacctggaaa gcctttacgg
     5221 cctgggaagg atggcacccg ttgtcgggtt gatttttaca gtatcggctc tttcgatatc
     5281 gggaatgccc ctcttttttg ggttcagagc gaagcttctt gcgatctcct cgagcttcga
     5341 atcgttgctc gtagttccga tcgtgatctt gctggccgcg gttatagaga taacttacta
     5401 cttcagatgg attttcgccc tttatagacc ttatcaagga gaggagaggg caaggctttc
     5461 tcttccgctg gagttaatgg ccataggcgt cgttcttgca gcgattctcg tgtttctggg
     5521 ctcaagctca tatgagactt tgatgacgtt cagcaaagcg ggcaacgcta tggtcgatgc
     5581 gatcatcaca ctgggaaaaa atctgattgg aggggtatga atgagcatcc tgaccctact
     5641 ggtggcttcg ttgctggctg caccgatctt ataccttttg gcgaagctca atacaaagat
     5701 cgcctacgga gcttatgctg cgcttcaggt cttttcgttc atttacgttt ttttcaccgg
     5761 aagaagtaac gtttcgtacg aagtgatcgg aatcaccgag aggctgaatt ttaacttcgg
     5821 catcactcca accaactggt tttttgcctc tatcgtgcta ctcataatat ccatgagtgc
     5881 aatcttcatg tttccactga agaagagtcc ggtgaagatt tttctgctcg ctcttgtctc
     5941 ggccggtacg cttggagcgg ttttttcaca agatttcctt acattaatga tcttctggga
     6001 gatttcgaca tgggcttcac tcatccttat aattcaggac aaggagaaat cgccaagaga
     6061 ggctataaaa tacgtcacaa tagcctcgat aggtacttat tcgatgcttt ttgcgatctt
     6121 ttatatgctc gataaattag gctcgataga cttctcgacc gttgccacga gaatcgaaag
     6181 tgcgactgtt gaggtacaat tggttatctt aatttccctg ggcgtgatgg ttctggcaaa
     6241 gatgggagtc tttccactgc atatctggct cagaggttct cactcttcgg ctcctgatga
     6301 atttagcccc atactctccg gtgccctcac caagataggc gcttttttgc tgttcacgat
     6361 caccgcagcc ttgccatctt tcaggatttt cagccagctt cccgttttca acggaatacc
     6421 catggtgaat tacactttcg ccctcctggg agggatatcc attgtagtgg gaacggttat
     6481 ggcaataagg atggaggacg caaaggaact gatagccttt tcaaccgtaa gcaattccgg
     6541 ttatatagtg atggcactct cgatcggggg cacatacgcc acagcaggag gcttgatgca
     6601 cgttctcaat catgccatgg cctcggcagc tatgttcatg gctatagccg ccgtagccta
     6661 tagaactggt acaacgaaaa tgcacgaaat gggtggcctg atactcaaaa tgcctgtaac
     6721 ctttgccact tacctggtgg caattatctc tgtagcgggg attccaccca ccagtggttt
     6781 cgtatcaaag tggcttatat accagcaact cgttcagaac ggtctcccct ttctggcctt
     6841 tgcagccttc tttggaagta taggttcgtt catgtacgtc ttcaaaccat tggccgggat
     6901 ctttctaggt caattgaagc cccagcatag aaatttaaag gaagtaccgc tcgtgatggt
     6961 tgtcccgatg gcaatcctca cactgttgac ggttttctgg gggatgctgc ccagtaacgc
     7021 cattaagact attaacgccg ttacggcttc ggtcggaatc aacgcggtag aggttacctt
     7081 ttccaggatc gtagcgctca ctggcgaatg ggattcgata ttggttacga ccgttttcgc
     7141 tattggattt ttcatatcac tcttcatatt catgagggcg aagaaagcca gacaggtaga
     7201 tctgatggac aactatacgg caggagattt tctatacacg agcgagttat accatttctc
     7261 gtacaggatg tacagaccct tcgacaggtt gttcgaaaaa tggccatcga tggaggactg
     7321 gctttcttct ctatcggata aaatcaagga actgggagcg ctgctgaagg cgatctttta
     7381 tccggccaat ccgcagctgt acataggtct cgcgatggtc gccattatcg ggaccttctg
     7441 gtggttgagg tgatcggtat gattataact attctatctg caatcgcact gctcatattg
     7501 gccttcgcct atactgtaac tctcgaagga atcgcgagga agatcgaggc tagaatacat
     7561 agaaggtgcg gtcctccatt ctggcagaac ttcatagata tcttcaagag tcttacaaaa
     7621 cattcgatct ctcatggttt catatttgat tttggagtcc ttatggccct gggtggcaca
     7681 atagccaccg tacttttcat accggcagga aatctcgtcg tcttccctgg tcttgacaac
     7741 gtcttcgtta ttatctatct gctggctata ggtctactgg gaatggcgat gtcggccgta
     7801 ggctcgggaa atcccaacgc ctcaatcggg ataggcaggg cattaacaca gatgctggga
     7861 tacgagctcc ccttcatgat agtgttgttg ggtgtcttct tccatttcag aacctcgtcc
     7921 ctatctgagc tggcagtact gcagaattcc ggaggaacct acaacgtctt gatcatgcct
     7981 atcggggcag ttgttggctt cgtatctctg atgggcatgc ttggcaagaa gcctttcgat
     8041 acatttattg cacctgccga gatagcttcc ggaccacttg tagagtacag cgggaaatac
     8101 ctgggattgc tcatgaccca gcacgctttc gggaccttta tagagattgg gcttttcgtc
     8161 aacctttttc tgggtggcgg aaagactatc tgggaatttc ttttgaagtt cttcctggtt
     8221 tactttcttg ccgttatcat ctcgtccatt atgccgcgtt tcagggtcga acaggcagtc
     8281 aaattctact ggaaatggcc actcatacta tccttcattc aagtggttat tgtcgttttc
     8341 ataatgggga ggcgagttta atgaacaacg agcagaccgc gtgggaaaga gtggcgaata
     8401 tgctgagggc aagatcgctt tggatgcttt attactgtac cggttgcggt gccatagaac
     8461 taccaccgac aatgacagcc cgcttcgata tggagaggtt tggtatagga ccgatggcga
     8521 caccgcgtca ggccgatata ctcctcgtaa cgggatacct cagcaccaag accctgcgca
     8581 gagtcatata ctcctacgag cagatgcagg ctccaaaata catagtcggg ttcggttcct
     8641 gcaccatcaa cggcggcatc tactatgact cttacgcgac gatcaacagg ctcgatcttt
     8701 acgtacccgt agatctatat ttggccggct gtatgcccag accagaggca attgtaaatg
     8761 gttttaaaaa tttgatggat aaaatcgacc aaggagaggc gacgggttgg aagga
//
LOCUS       DAOOLY010000082         8729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9436_length_8729_cov_6.118976, whole genome shotgun sequence.
ACCESSION   DAOOLY010000082 DAOOLY010000000
VERSION     DAOOLY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9436_length_8729_cov_6.118976"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLD15_05670"
     CDS             <1..319
                     /locus_tag="PLD15_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPB63426.1"
                     /translation="GNTSGFCVPTFVVDAPGGGGKIRVMPQYNISESDRVVVLRNYEG
                     VITTYHEPEDTVSDVDDGLYKEKYTFTGVSDLLYGNGLSFEPTVLQRRERIKLWKEKR
                     VLK"
     gene            316..726
                     /locus_tag="PLD15_05675"
     CDS             316..726
                     /locus_tag="PLD15_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPB63427.1"
                     /translation="MIYDEFTVGQKYAVKKIITTEMVDAFAAITGDKNPVHVDGEFAK
                     TTRFGRRIAHGMLSVGIISAILGNDFPGPGSIYMKQDVKFFKPIYLEEEVEIEIELLE
                     KIEEKKRLLVRTTVKKSSGEIAVDGEALLLFTQE"
     gene            689..1723
                     /locus_tag="PLD15_05680"
     CDS             689..1723
                     /locus_tag="PLD15_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63428.1"
                     /translation="MTGRRFSSSRKSEKVSRLIELASLHPVVSIVGMEKNTGKTVVLN
                     HLIKGLSGESDVTGITSVGLEGERYDQVYLTEKPEITLRRGNLFATSEKDFRSRRFPA
                     TVLDVREFRSPLGRTVFARARSDGKVIISGPSIVDYLSQTVRMLREFGARRVLVDSST
                     SRLSPGSPAVVDAIILATGAALSISPGEIVRRTIHQVKIITLPGVEKEAGDIMEGLGN
                     GVWTVDGPEVKLLAVSSLSMESGLDLSGRTVYVNGSLTSRALGKLAAPGVRVVVRDFS
                     RIFVDEESLRSFQRHGGSVNVLHSARLLAVTLNPVSPSGYSLRSGQLKETLERFLPVP
                     VFNILEEEDR"
     gene            1720..3096
                     /locus_tag="PLD15_05685"
     CDS             1720..3096
                     /locus_tag="PLD15_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB63429.1"
                     /translation="MNLSAGCGFDYIKGLVETLTPMGRKRLYDISFLTERGRIEMEYD
                     RQEDLKEMAANPDKVVSIISRFRDISATLENLAAGAVLDDVELFEIKSFAIWNEKLRE
                     RLGDCPGWMRLPDLSTVIDLLDPEKTGLESFYISDLFDGSLPELRRKIAQCQRSDDPH
                     DRERAADLLEMNARVESKVRARLSKELEGFSDTLSLSLNRVGDIDLTLAKAILNERLK
                     LARPSIVEDAFSFEGLFNPEVASVLSEKNKEFQSYSLSLRRLALVIGGNMTGKSLFLR
                     TLALAQTMFQHGFFVPAKEASLTPYDRIVYYSGDHQSFENGLSSFAGEVEFLKEAVGI
                     VSRGERGLFLFDEIGKNTNPLEGPAFLLAAMEYLGRGNGCRAVFSSHYEEALENREIQ
                     MLMVRGLDHEKLAGRERRDISFYIDHTVQEIDEFCGFPREGTVIARLLGMDDDFLKLV
                     EKHLWRKE"
     gene            3093..4652
                     /locus_tag="PLD15_05690"
     CDS             3093..4652
                     /locus_tag="PLD15_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPB63430.1"
                     /translation="MKSKLDLDFRKVERARKLAVSIADGVQNFVAGRTTVSVERAVCR
                     LMGIDGVDSYGVPLPNVVVEHLASRNLLQEGASYCIANAMMKTGLSPQRIAEEISAGR
                     LDLLSMSYGGEEEVYGFLDPLAQRAVEKVRDRRSEREALIDRLAEGPRPYLYVIVATG
                     NIYEDVVQARAAVSQGADIVAVIRSTGQSLLDYVPYGPTTEGFGGTYATQENFRIMRA
                     ALDEMGEKVGKYIRLCNYCSGLCMPEIAAMGAMERLDVMLNDALYGILFRDINMQRTL
                     IDQFFSRVINGFAGVIINTGEDNYLTTADAYQEAHTVLASQFINERLALIAGIPEEQM
                     GLGHAFEMDPDIENGFLYELAQAQMSREIFPRAPLKYMPPTKFMTGNIFKGYVQNALF
                     NIVSIWTNQGIQLLGMLTEAIHTPFMGDRYLAIENAKYIFNNMRALGEEVVFKKDGII
                     HARANQVLDQALELLAEIEEDGLFRALERGVFAGVRRPVEGGKGLPGVVLKGERYRNP
                     FLEKMLGGYCR"
     gene            4649..5434
                     /locus_tag="PLD15_05695"
     CDS             4649..5434
                     /locus_tag="PLD15_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPB63431.1"
                     /translation="MSGYSLENKKPDRALDPTGIKPYGDTMNDGKVQLSFTLPVQNGP
                     EAVEAARLLLGKMGFKEPQIVFSREISEGFTFFVAYGVSIHEVDYSSIKVPKLSLEKM
                     SMEEIDRFIDERIRRKVVVVGASTGTDAHTVGIDAIMNMKGFAGHYGLERYRNFEAYN
                     MGSQVPNEELVAKAIEVGADAILVSQTVTAKNVHLKNMTDLVELLEAENLRKKIILIA
                     GGARITHELAKELGYDAGFGPGSFAEDVATFIVQKLETMIGNL"
     gene            5474..6376
                     /locus_tag="PLD15_05700"
     CDS             5474..6376
                     /locus_tag="PLD15_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPB63432.1"
                     /translation="MKTLVVVNPNASHGESGKDFETTIKGMIREKLGDFDLHVTTGQR
                     DAMSFVSDHGDYERIISVGGDGTLNEVVNGIMNSRSGAMLGMIAVGSGNDFARSIDLT
                     RDYGKMVDIACGSKARHVDLFEVRYRAFSGEKAARFAVNVVGTGFDAAVTNRMNRSRF
                     KTSGKMAYLLSFLIEFLTTRTYRLNYTIDSGEFSDRYYFLTMGNGNYFGGGMRIAPNA
                     VVDDGLIDIIGVAKMAKIRLLYHFPKIYKGEHLKIDAVCHHVSERISVSSDRDVIIQM
                     DGEVVGKLPMEISVHKKTLCILSA"
     gene            complement(6368..7243)
                     /locus_tag="PLD15_05705"
     CDS             complement(6368..7243)
                     /locus_tag="PLD15_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63433.1"
                     /translation="MKLAMGLIFVLVGILLIFGIFTGDTLLNAFVNLINYWPIILVFI
                     GLSILSGVKGLKWLKYLNALLTVCFILFIFFWPSAFLSGSRTVSETTLLETAEELTVV
                     EIQIDLPMVNIRIEAMEAAAPLSTVAHVDYKIRGAGLKIEESGSATLKKWTLKADSNL
                     SWLTSSTMVLKLNPAYKYIIKLNTAAVNGSFDIAGLKVEELDVNCAVLKADFRVPAVS
                     NSRIEVKTAISNGDIFVPENVRAFLRARAAIKNVKSDLNKTGSGEEYIYEGTGNEYNT
                     NLSFESAILNLKVLR"
     gene            complement(7248..7691)
                     /locus_tag="PLD15_05710"
     CDS             complement(7248..7691)
                     /locus_tag="PLD15_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPB63434.1"
                     /translation="MDTRKRLYKSRRDKVIDGVAGGVASYLGIDPVIVRLIFVALIFA
                     GGAGLIIYIIGMFIIPREPLNSENNVVIMDEEGKPIEDKPGVEISDDKSKIIIAVVLI
                     IFGIALLLGTFTPWNIFSGVFWKFVIGAVLVAGGGFVIYKSINRR"
     gene            7847..>8729
                     /locus_tag="PLD15_05715"
     CDS             7847..>8729
                     /locus_tag="PLD15_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63435.1"
                     /translation="MQFILVIPAITIVVFIGFLPIFTAFSDAVIINGTPSLLNFHYIL
                     QDQGFMYSLKISLVWALFNTLLSLSLGFLIAHHLNRARRWRLIYLALLVPWGLPVYIG
                     VPIWRAIVHGGGGTSILNLIGIDINLLTDPVASFLAALFVSVWFSLPLTVFVLWGSMA
                     TVQTSLVDAAKIDGGSLSTEAFSIYLPMMKPALITMFTINFIKFLKEFTVVFLMTDGG
                     PPLVSGFTKRSIIGATTTLDIFIYDIFTGRSSTGIASAYSVLTLLLVAFVMILWFLSS
                     RVELNAHLLIILVVAAQT"
BASE COUNT         2099 a         2161 c         2488 g         1981 t
ORIGIN      
        1 cggaaataca tccggcttct gcgttccgac tttcgtcgtt gatgctcccg gtggcggcgg
       61 caagatcaga gtcatgccgc agtacaacat ctccgaatcc gacagggttg tggtgttgag
      121 aaattacgag ggcgttataa cgacctacca cgaaccggag gataccgtca gcgatgtcga
      181 cgacggtctt tataaagaga aatacacctt cacgggagtt tccgatcttc tttacggaaa
      241 cggtctcagt ttcgagccaa cggttttgca gagacgcgaa agaataaaac tctggaagga
      301 aaagagagtg ctgaaatgat atacgatgag ttcactgtag gtcaaaagta cgccgtcaag
      361 aagataataa cgacggaaat ggtcgatgcc tttgccgcta taacgggcga taaaaatccg
      421 gtccacgtgg acggagagtt cgcgaaaacg acacgtttcg ggcgtcgaat cgctcacggt
      481 atgctgtcgg tggggataat ttccgccata ctgggcaacg actttcccgg gcccggttct
      541 atatacatga agcaggacgt gaagtttttc aagccgatct atctcgaaga ggaggtagag
      601 atcgagatag agctgctgga gaagatcgaa gagaagaaga gactgctggt gaggaccaca
      661 gtaaagaaat catccggaga gatcgccgtt gacggggagg cgcttctcct cttcacgcaa
      721 gagtgaaaaa gtgtcacgcc tgatcgaact tgcaagcctt catccggtcg tctcgatcgt
      781 cgggatggag aagaacaccg gcaagacggt cgttctgaac catcttataa agggactgtc
      841 cggcgaatcg gacgtcaccg ggataacctc ggtcggtctg gagggggaga ggtacgatca
      901 ggtatatctc accgaaaaac ccgagattac tcttcgcagg ggcaaccttt tcgcaacgtc
      961 tgaaaaggat ttccggtctc gccggtttcc ggccaccgtt ctggatgtca gagagttccg
     1021 ctcccctctg gggcgcaccg ttttcgcgag ggcgcggtcc gacggaaaag tcataatctc
     1081 cggcccttcc atagtagatt acctatcgca aacggtgagg atgctcagag agttcggcgc
     1141 tcggagggtc cttgttgaca gttctacgtc cagactgagc cccggctcgc cggcggtggt
     1201 cgacgccata attctggcga cgggtgcggc cctttcgatc agtccgggag agatcgtgag
     1261 gagaaccata catcaggtga agatcatcac tctgcccggg gtggaaaagg aagcgggcga
     1321 tataatggaa gggctcggta acggcgtctg gactgtggat ggaccggaag tgaaactcct
     1381 cgcggtttcg agtctttcca tggagtcggg cctcgatctt tcagggagaa ccgtttacgt
     1441 gaacggttcg ttgacttcca gggctcttgg aaagctcgcg gcgccgggtg tcagggtggt
     1501 ggtcagggac ttctcgagga tcttcgtcga cgaagaatcg ttgagatctt tccagaggca
     1561 cggcggcagc gttaacgtgc tccacagtgc gcgacttctg gccgttactc tcaatcccgt
     1621 atcgccttcg ggttactccc tgagatccgg tcagctgaaa gaaacgctgg agaggttttt
     1681 gccggtaccg gtcttcaaca ttttggagga ggaagataga tgaatctttc ggccggctgc
     1741 ggttttgatt atataaaggg actggtcgag acattaaccc caatgggtag aaagaggctt
     1801 tacgatattt cttttctgac tgagcggggc cggatagaga tggagtacga ccgtcaggag
     1861 gatctgaaag agatggcggc caaccccgat aaagtcgttt cgatcatctc gaggttcagg
     1921 gatatctcgg ccactctgga aaacctggcg gccggagcgg tgcttgacga cgtggaactc
     1981 ttcgaaataa agagcttcgc gatctggaac gagaaactca gagaacggct cggcgactgt
     2041 ccgggctgga tgagacttcc cgacctttcg accgtgatcg atcttctcga tcccgaaaag
     2101 acggggctgg agagtttcta catctccgat ctcttcgatg gttcgctgcc ggaactgagg
     2161 aggaaaatcg cgcaatgtca gaggagcgac gatccgcacg acagggagag agcggccgat
     2221 ctgctggaga tgaacgctcg ggtggagtcg aaagtcaggg cccggctctc gaaagagctg
     2281 gaaggctttt cagatactct atcgctttct ttgaacaggg tgggcgatat cgatctgacg
     2341 ctggccaaag cgatcttgaa tgagaggctc aaactggcga gaccttcgat agtggaggat
     2401 gccttctctt tcgaaggtct tttcaacccc gaagtggcca gtgttctttc ggaaaagaat
     2461 aaagagttcc agagctattc tctctctctg cggagattgg ccctcgtgat cgggggcaat
     2521 atgacgggga agtctctctt tttgaggacg ctggcgctag cccagacgat gttccagcat
     2581 ggcttcttcg ttccggcgaa ggaggcttcg ctgactccgt acgacagaat agtctattac
     2641 agtggcgatc accagtcgtt cgaaaacggc ctctcttcct tcgccggaga ggttgagttc
     2701 ttgaaggaag cggtcggtat cgtctcccgt ggagagaggg ggctctttct cttcgacgaa
     2761 atcggaaaga acaccaatcc gctggaagga ccggccttcc tgctggcggc gatggagtac
     2821 ctcggtcgcg ggaacggttg cagggccgtc ttctcttcgc actacgaaga ggcgctagaa
     2881 aacagggaga tccagatgct catggtcaga ggtctggacc acgagaaact cgcaggtcga
     2941 gagcgacggg atatctcttt ctatatagat cacacggtac aggagataga tgaattttgc
     3001 ggctttccga gggagggaac ggtcatagcc cggctgctcg ggatggacga tgattttcta
     3061 aagctggtag aaaagcactt atggaggaaa gaatgaaaag taaactcgat ctcgattttc
     3121 gaaaagttga acgggcaaga aaactggcgg tgagcatcgc cgacggcgtt cagaatttcg
     3181 tggccgggag aactaccgtg agcgtcgagc gggccgtttg ccggctgatg ggtatcgacg
     3241 gggtggactc ttacggggta ccgttgccga acgtcgtcgt agagcatctg gcatccagga
     3301 acctgctcca ggaaggcgct tcgtattgca tcgcgaacgc catgatgaag acgggactat
     3361 ctccccagag aatagccgaa gagatatcgg ccggccggct cgacctgttg agcatgagtt
     3421 acggcggaga agaagaggtc tatggttttc tggatcccct ggcccaaaga gccgtcgaga
     3481 aggtacgcga cagaaggtcc gagagagagg ctctcatcga cagactcgct gaaggaccgc
     3541 ggccttacct gtacgtgatc gtagcgaccg gaaacatcta cgaggacgtc gttcaggcac
     3601 gggcggccgt aagtcaggga gccgacatag tggcggtaat aagatcgacc ggccagagtc
     3661 tgctggatta cgtcccttac ggccccacga cggaaggctt cgggggaacc tacgccaccc
     3721 aggagaactt caggatcatg agggccgccc tcgacgagat gggcgaaaag gtaggtaaat
     3781 atatacgtct atgcaactac tgttcgggtc tgtgcatgcc ggagatcgcc gcgatgggtg
     3841 cgatggaacg tctcgacgtg atgctgaacg acgcccttta cgggatactc ttcagggata
     3901 taaacatgca gcggacattg atcgaccagt tcttctccag agtcataaac ggcttcgcgg
     3961 gggtgataat aaacaccggc gaggacaact atcttaccac cgccgacgcc taccaggaag
     4021 cccacacggt tctggcgtcc cagttcataa acgagagact ggccctgatt gccggtattc
     4081 ccgaagaaca gatgggactc ggtcacgcct tcgagatgga cccggatatt gagaacggct
     4141 ttctgtatga gctggcccag gctcagatga gcagggagat ctttccccga gcaccgctga
     4201 agtatatgcc ccccaccaag ttcatgaccg gtaacatctt caaaggttat gttcagaatg
     4261 ccctgttcaa catcgtttcg atctggacca accagggaat acagcttctg ggaatgctga
     4321 ccgaggcgat acacacgccc ttcatgggag acaggtatct ggccatagag aacgcgaagt
     4381 acatattcaa caacatgcgc gcgttggggg aggaagtggt cttcaagaag gatggaataa
     4441 tccacgccag ggcgaaccag gtgctcgatc aggcactcga gcttctcgcg gaaatagagg
     4501 aagacggtct cttcagggcg ctagagaggg gagtctttgc cggggtcagg cgaccggtcg
     4561 agggaggcaa gggactgccg ggagttgttt tgaaagggga gcgttacaga aatccattcc
     4621 ttgagaaaat gcttgggggg tattgcagat gagcggttac tctttggaaa acaaaaagcc
     4681 tgatagagcg ctggatccaa caggaatcaa gccttacggc gacacgatga acgacggaaa
     4741 ggtccagcta agcttcactc ttcctgtaca gaacggaccg gaggccgtcg aggcggccag
     4801 gcttcttctg ggaaagatgg gtttcaaaga gccgcagata gtcttttcca gggaaatatc
     4861 ggaaggcttc accttcttcg ttgcttacgg cgtgtcgata cacgaagtcg attactcgtc
     4921 aataaaagtc ccgaaactct ccctcgagaa gatgtcgatg gaagagatcg acagattcat
     4981 agacgagagg ataagaagaa aagtggtggt ggttggcgcc agcacgggaa ccgacgccca
     5041 tacggtcggg atagacgcga taatgaacat gaaaggcttc gccggtcatt acgggctgga
     5101 gaggtaccgc aacttcgagg cctacaacat gggaagccag gtgccgaacg aagagctggt
     5161 ggccaaagct atcgaggtcg gagccgatgc catactggtc tcccagacag ttacggcgaa
     5221 gaacgttcat ctgaagaata tgaccgatct cgtcgaactt ctggaggccg agaatcttcg
     5281 caaaaagata attctcatag cgggcggagc caggatcact cacgaacttg ccaaggagct
     5341 cggttacgac gccgggttcg gaccgggttc gttcgccgag gatgtcgcga ctttcatcgt
     5401 acaaaagctc gagaccatga ttggaaatct gtgatagaat ttcttatcgt tgactttctg
     5461 ggggtagatc cttatgaaga ctctggttgt cgttaatccc aacgccagtc acggtgaatc
     5521 ggggaaggat ttcgaaacga ccataaaggg aatgataagg gagaaactgg gagatttcga
     5581 tctgcatgtc actaccggcc agagagacgc gatgtctttc gtgtccgatc acggggatta
     5641 cgagagaata ataagcgtcg ggggcgacgg tacgctgaac gaggtcgtga atggaatcat
     5701 gaacagccgg tccggcgcca tgctcggcat gatagcggtc ggttcgggca acgacttcgc
     5761 cagaagcatc gacctgacgc gggactacgg caagatggtc gatatcgcct gcggtagcaa
     5821 agccaggcac gtcgatctct tcgaagtcag gtacagagcc ttctccggcg aaaaggcggc
     5881 gagattcgcc gtgaacgtcg tgggaacagg tttcgacgcg gcggtgacca atcggatgaa
     5941 ccgctcgagg ttcaaaacca gcggtaaaat ggcctacctc ttatcgttct tgatcgaatt
     6001 tttaaccacc agaacttaca ggctcaacta caccatagac agcggcgagt tctccgacag
     6061 gtactacttt ttgaccatgg gcaacgggaa ttacttcggc ggaggcatga ggatagcgcc
     6121 gaacgccgtc gtcgacgacg ggctcataga cataatcggg gtcgccaaga tggccaagat
     6181 caggttgctg taccactttc ccaagattta caagggcgag catctgaaga tagacgcggt
     6241 ctgtcatcat gtatcggaga gaatttccgt gagcagtgac agggacgtta tcatacagat
     6301 ggacggagaa gtcgtcggga agctcccgat ggagatctcc gtccacaaaa agactctctg
     6361 tattctttca gcgtaatact ttcagattga ggatggcgct ctcgaaggag aggttggtgt
     6421 tgtattcgtt tccggtgcct tcgtagatgt attcctctcc cgaaccggtc ttgttgaggt
     6481 cggacttcac gttctttatg gccgccctcg ctctcaaaaa ggcccgcaca ttttccggca
     6541 cgaagatatc gccgttcgag atcgcggtct taacttcgat cctggagttc gagacggcgg
     6601 ggactctgaa atcggccttc agtacggcac aattgacatc cagttcctcg actttcaaac
     6661 cggcgatgtc gaaactcccg ttgacggccg ccgtgttcag cttgattatg tatttgtagg
     6721 ccgggttgag cttcaaaacc atggtgctcg aagtcagcca ggatagattc gaatcggcct
     6781 taagggtcca tttcttcaaa gtcgcactgc cgctctcctc gatcttcaga ccggcgcccc
     6841 tgatcttgta atccacatgg gctacggtgg aaagcggagc ggccgcttcc atagcttcga
     6901 tccttatgtt caccatgggt agatcgatct gtatctccac aaccgtgagt tcttcggccg
     6961 tttccagcag ggtcgtttcc gatacggttc tgctgccgga aaggaaggcc gaaggccaga
     7021 agaagatgaa gagtatgaag cagaccgtta gcaacgcgtt caagtacttc agccacttga
     7081 gtcccttcac tcctgacagt atgctcaacc ctatgaaaac gaggattatg ggccagtagt
     7141 taataaggtt gacgaaggcg ttcaaaagag tgtcgccggt gaagataccg aaaatcagca
     7201 gtatccccac aagtacaaat atcaacccca tcgcgagttt catgctatca tctcctgttg
     7261 atggacttgt agatgacgaa tcctccaccg gccaccagaa cggctcctat gacgaacttc
     7321 cagaacacgc cgctgaagat attccatggt gtgaatgtgc cgagcaagag agcgataccg
     7381 aatattatca gcacaacggc tattattatc ttgctcttgt cgtcggatat ctccacaccg
     7441 ggtttgtctt ctattggttt gccttcctcg tccattatga caacgttgtt ctcgctgttc
     7501 aaaggttcac gcgggattat gaacataccg atgatgtaga ttatcagtcc ggctccgccg
     7561 gcgaaaatca gggctacgaa tatcaggcga actatcaccg gatcgatccc cagataactg
     7621 gccactccac cggcgacgcc gtcgatcact ttgtctcttc gcgatttgta aagcctcttc
     7681 ctcgtgtcca agagaaacac ctcctgtcag gatttgtcgt aaaatcctcc ttgaaaatgg
     7741 acattagcat tataatatgc gtaccgttaa atttatactt gttcattgat ttttgaattt
     7801 tttcagcgaa acatcccgtt ggttcggagg ttttcgggag gttgagatgc agttcatcct
     7861 cgttattccc gcaataacaa tagtggtatt tatagggttc ctgccgatct tcacggcctt
     7921 ttccgacgcc gtgataataa acggaacacc gagccttttg aacttccatt acatacttca
     7981 ggatcagggt ttcatgtaca gcctgaagat ctcactcgtg tgggcccttt tcaacaccct
     8041 gctatcgctc tcgctgggct ttttgatagc ccatcacctc aacagggcca gaagatggcg
     8101 attgatctac ctggcactgc tggttccgtg gggactgccg gtctatatcg gtgtgccgat
     8161 atggagagcc attgtgcacg ggggaggcgg cacctcgata ctcaatctga tcggcatcga
     8221 tataaacctt ctcaccgacc cggtcgcgtc gttcctcgcc gccctcttcg tcagcgtgtg
     8281 gttcagcctg ccgctgactg tcttcgttct ctggggttcg atggccaccg ttcaaaccag
     8341 tcttgtcgat gccgcgaaga tcgatggcgg gagcctgtcg acagaggctt tcagtatcta
     8401 cctccccatg atgaagccgg ccctgataac catgttcacg atcaacttca taaagttctt
     8461 gaaggagttc acggtggttt ttctcatgac cgacggaggt cctcccctgg tgtcgggctt
     8521 cacgaagagg agcataatcg gcgccactac aacgctggat atatttattt acgacatttt
     8581 caccggtcgc agctcaacgg gcatcgcctc ggcttactcg gttttgacgc ttctgctggt
     8641 ggccttcgtg atgattctct ggttcctctc gagcagggtc gaactgaacg ctcaccttct
     8701 gattatactt gtggtagcgg cgcagacca
//
LOCUS       DAOOLY010000083         8671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9523_length_8671_cov_4.557219, whole genome shotgun sequence.
ACCESSION   DAOOLY010000083 DAOOLY010000000
VERSION     DAOOLY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9523_length_8671_cov_4.557219"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /gene="dnaN"
                     /locus_tag="PLD15_05720"
     CDS             complement(<1..1097)
                     /gene="dnaN"
                     /locus_tag="PLD15_05720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPB63436.1"
                     /translation="MNFAVERNEILTRLEAVSGAAASKTVKPILSGIYFSVKGEFVIQ
                     ITATDLETAIKTEVKVKHIDGQDGFVVDAKVALEIVRNLPEGEIIFEVEETNLIIRAG
                     KSRFTLPTMDPEEFPDVDTTTGGVEFSVSVSAIELMIDRVIFCAARDEFMRNLNGVYW
                     EFEGGFLRLVAADGFRMSLAEERIDVSVDDHFLLTLKSMRDLQNSMKSAMSDEMKIVY
                     DGSRVGFYFDGTEMVSKVVDAEFPDYKRVLPKSFKSRVQLYTSNLSDAVRRASIAARL
                     GSDSVKLDIKDELFRIIARSPDHGEAIEEIEGKKEGDNLVIAFNPRFLSESLRKIDTE
                     EVELNFVDSNSPLQINPIDVQGYTYIIMPIRL"
     gene            1207..1884
                     /locus_tag="PLD15_05725"
     CDS             1207..1884
                     /locus_tag="PLD15_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HPB63437.1"
                     /translation="MKVKDLHSWNMDRSDAVEIQKLLKENIVIRDNPIEPSLVAGVDL
                     SFPDKSTGLAVVAVLNFPELELLEYSYHVGKVERPYIPGLLSFREGPEIIEALKNTPE
                     VDLVFFDGHGTAHPRGIGIASHIGLFLEVPTIGVAKSLLYGKCELPGSSRGDSSPILS
                     PAGELIGYSLRTKPGVKPVYVSPGNGLSMKQALEFSLKATGIYRLPEPTRQAHILSQK
                     LKAQLSL"
     gene            complement(1869..2819)
                     /locus_tag="PLD15_05730"
     CDS             complement(1869..2819)
                     /locus_tag="PLD15_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB63438.1"
                     /translation="MSDLVVGVDLGGTETKIGIVEENGRILSKSIIPTKVFEGRIAVV
                     TRIVEAIKQLIMETGISVDGVVGIGVGSPGSIDHETGTVLFSPNLPDWSGFELTAILK
                     KLTGIRTFVENDANAFVLGEWAFGAFKGCKHMIGLTLGTGVGGGVITHGTLMTGKNGY
                     GGELGHTIVQIGGPLCGCGSHGCLEALASATAVVNMAREYSKRFSKSLIYQAQQLTAR
                     VVFDCAREGDLAASMIVERVTDALAAAIGNFVHIFNPEHIVIGGGMSRAGNILIDGVR
                     RKLPRFVMPSFSETFQVDLSDLVENAGITGAASIVFYRLS"
     gene            complement(2835..4097)
                     /gene="rho"
                     /locus_tag="PLD15_05735"
     CDS             complement(2835..4097)
                     /gene="rho"
                     /locus_tag="PLD15_05735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPB63439.1"
                     /translation="MDISQLQNMPIREIYKLARKYDVANYTQMAKKDLIFAVLKAQTE
                     SYGYFFDQGVLEITDGGYGFLRTLENNLLPSSNDIYVSQSQIRRFNLTSGDTVAGQVR
                     PPKEGEKYFALLRIEAINHKAINFAAERITFQNLTPIFPEEQLMSESDTHVLSTRMLD
                     LFSPIGKGQRGLIVSPPKAGKTVLLKELANGIAENHPDTIRIILLIDERPEEVTDLKR
                     TTSAHVLAAPFDMHPEKQIKVAEMTLEMAKRLVEFNYDVVILLDSITRLARAYNLYVP
                     PSGKLLSGGVDPSALYKPKHFFGAARNIEEGGSLTIIATALVETGSKMDEVIFEEFKG
                     TGNMELVLSRQLANKRMFPAINLTLSGTRKEELLLPQDILRKVWVLRRMLSAMSEEEG
                     LRLITDKLRGTASNEEFLDLIEMEKKQY"
     gene            complement(4874..5530)
                     /gene="fsa"
                     /locus_tag="PLD15_05740"
     CDS             complement(4874..5530)
                     /gene="fsa"
                     /locus_tag="PLD15_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPB63440.1"
                     /translation="MKIFLDTANIEQIRNGMKLGLVDGVTTNPTLVSKENAKFEERIV
                     EICEAVKGPVSAEVIATDFDNMLSQARELASLNEHVVVKIPMTPDGIRAVKVLSEEGI
                     KTNVTLVFSSLQGLLAAKAGATYVSPFVGRLDDVGNDGMQLVSEMDEIFGNYGFATEI
                     IVASVRHPMHVLQAALIGADIVTIPYDVLLRLFNHPLTDNGLKRFQSDWQEYERKQRP
                     "
     gene            complement(5569..6015)
                     /gene="rplQ"
                     /locus_tag="PLD15_05745"
     CDS             complement(5569..6015)
                     /gene="rplQ"
                     /locus_tag="PLD15_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPB63441.1"
                     /translation="MRHRVSGSKLNMPHSQRVSLMKNLARELFEHGTIVTTVTKAKEL
                     RPFVESIITRAKKATTITQELGTKSAESPEAIELKSRNLALRREINRNFNDRRLVKKI
                     CDEVAVKYLTRNGGYTRIVKLGMRRGDASEMAVIQLVDNEEKKETK"
     gene            complement(6037..7056)
                     /locus_tag="PLD15_05750"
     CDS             complement(6037..7056)
                     /locus_tag="PLD15_05750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPB63442.1"
                     /translation="MIASIMPKNLRVEEEKEDQNSYYTRFVLSPMERGYAITIGNSLR
                     RVLLSSIPSMAITKLKIPGKYHEYDVIEGVKEDILEIILNLKKVQLKPALEFSDPVKL
                     TLEKVGPGVVSAGDIKTPTGVEVVNPSQYIATLNTDSIVYIELFAEIGKGFVPVSEME
                     IDHDVEMIYIDGIFSPVMKVNFQTENVRVGKRTDYDKLVLEVWTKKSTKPSEALLRAT
                     DILTRHFEVVSNGLGQQNQVSIVTPAEPEEEELVQEETQIASESPQTEEVDSNPLTSK
                     RVEELDLSVRSLNCLKRDKINTIGDLIDKTEADLLKIRNFGEKSMLEVVKKLRDKFNI
                     ILKKE"
     gene            complement(7111..7737)
                     /gene="rpsD"
                     /locus_tag="PLD15_05755"
     CDS             complement(7111..7737)
                     /gene="rpsD"
                     /locus_tag="PLD15_05755"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPB63443.1"
                     /translation="MARYTGPVCKLCRREGFKLYLKGERCFSPRCGQIKRPVAPGQHG
                     ASTRKLTQYGMQLRSKQVVKRIYGVLERQFRRYFEMAVRKHEETGNALLKILESRLDN
                     TVFRMGFASSRRQARQLVSHGHITVNGRRVNKPSFNLRVGDIVEIKEKSRVVLPVKEA
                     VEAAKERAAYPWLEINYEDFRGTYLRYPAREEVEIPVDLQSIIELYSK"
     gene            complement(7754..8140)
                     /gene="rpsK"
                     /locus_tag="PLD15_05760"
     CDS             complement(7754..8140)
                     /gene="rpsK"
                     /locus_tag="PLD15_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPB63444.1"
                     /translation="MAKRPAAKKRRKLTSDKGIVHIKSSFNNTIITLTDPDGNTLMWT
                     SGGTAGYSGSKKSTPYAAQLGADKIAKEAIKLGITRVGIEVKGPGSGREAAIRTIQAA
                     GLTIETLKDITPLPHNGCRPRRRRRV"
     gene            complement(8164..8532)
                     /gene="rpsM"
                     /locus_tag="PLD15_05765"
     CDS             complement(8164..8532)
                     /gene="rpsM"
                     /locus_tag="PLD15_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPB63445.1"
                     /translation="MARILGVELPNNKKTFVALTYIYGIGYTRANEILAGTEIDGDKR
                     AKDLTDEEVSKIAKFINEHYKVEGELRTEVDRSIKRLIEIGSYRGYRHRNGLPVRGQK
                     THSNGRTRKGSRSSKIRKKS"
     gene            complement(8545..8661)
                     /gene="rpmJ"
                     /locus_tag="PLD15_05770"
     CDS             complement(8545..8661)
                     /gene="rpmJ"
                     /locus_tag="PLD15_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPB63446.1"
                     /translation="MKVRASVKKRCEHCKIIRRNGRIRIVCEKNPKHNQRQG"
BASE COUNT         1899 a         2332 c         1914 g         2526 t
ORIGIN      
        1 agccttatag gcataattat ataagtataa ccctgaacat cgatgggatt gatctgcaac
       61 ggactgttgg aatcgacgaa attcaactcc acctcttctg tatcgatttt tctcaaagac
      121 tccgaaagaa acctcggatt gaatgcgatg acgaggttgt cgccttcttt ctttccttct
      181 atctcctcga tcgcttcacc gtgatccgga ctgcgcgcga ttattctgaa tagctcgtcc
      241 tttatgtcga gcttgacgga atcggaaccc agcctggcgg cgatcgaagc tcttcgaacg
      301 gcgtcggaga gatttgaagt gtacaactgg actctcgact taaaagattt tgggaggact
      361 ctcttgtagt cgggaaactc ggcgtcgaca actttggaaa ccatctccgt tccatcgaag
      421 taaaacccca ccctgctgcc gtcgtaaact atcttcattt cgtcagacat tgcgcttttc
      481 atactattct gaagatctct catgctcttg agtgtcagaa ggaagtgatc gtcaacgctc
      541 acatctattc tctcttctgc gagcgacatt ctgaagccat cggccgctac gaggcgaagg
      601 aaccctccct cgaattccca gtaaacgccg ttgagatttc tcataaactc atctctcgcg
      661 gcacagaata ttacacgatc gatcatcagt tctatcgcgg aaaccgatac tgaaaactcg
      721 acgccgccgg tagttgtatc cacatcggga aactcttcgg gatccatcgt tggcagtgtg
      781 aatctgctct taccggctct tatgattaga tttgtctctt caacctcgaa tataatctct
      841 ccctcgggta ggtttctgac tatttcgaga gctaccttcg catcgaccac aaaaccgtcc
      901 tgcccatcga tatgtttaac cttgacttcc gtcttaatag ccgtctcgag atcggtggcc
      961 gtaatctgaa tgacgaactc gcctttcacg gagaaatata tccccgaaag tatgggtttg
     1021 accgtctttg aagcagcggc gccggaaaca gcttcaagtc gcgtgagtat ctcgttgcgt
     1081 tcaactgcaa agttcatcgg tacacctcca cttaatgggc ttttgataaa ttataacatc
     1141 tgtatggcac aataattatt agtcatccgc tcattagtgg tacatggcgg ttatacggag
     1201 gagttcatga aagtaaaaga tcttcactcc tggaatatgg atagaagtga tgccgtcgaa
     1261 atacagaaac ttctgaaaga gaacatcgta ataagagata accctatcga accctccctg
     1321 gtcgccgggg tagatctttc ctttccggac aaaagcaccg gtctggccgt ggtcgcagtc
     1381 ttgaacttcc ctgaacttga actgctggaa tactcgtacc acgttggaaa ggtcgaaaga
     1441 ccgtatatcc ccggtctctt atcattcagg gaaggaccgg agatcataga agcccttaaa
     1501 aacaccccgg aagtcgatct ggtttttttt gacggccatg gaacggccca cccaagaggt
     1561 ataggtatcg cttcacacat cgggcttttt ctggaagttc ccacgattgg agtggcaaaa
     1621 agcctcctct acgggaagtg cgaacttccg gggagctccc ggggcgacag ttcacccata
     1681 ctctcaccgg ccggtgagtt gataggttat tctttgagaa cgaaacccgg cgtcaaaccg
     1741 gtgtatgtct ctcccggaaa tggactctct atgaagcagg cccttgaatt ttctttaaaa
     1801 gccaccggta tctacaggtt gccagaaccg accaggcagg ctcacatact cagtcagaag
     1861 ctcaaagctc agctcagcct gtagaaaact atcgaagccg caccggttat accggcgttt
     1921 tcaaccagat cactgaggtc gacctggaat gtttcgctga aagacggcat tacgaaacgc
     1981 ggcagttttc ttctgactcc atctatgagg atgtttccgg ctctgctcat gcccccgccg
     2041 attactatat gttcgggatt gaaaatatgc acgaaattac cgatcgcggc ggccaatgca
     2101 tccgtaactc tttcgactat catagaggcc gcgaggtcgc cttccctcgc acaatcgaac
     2161 acgaccctgg ccgtcagttg ctgagcctgg tagatgagcg acttcgaaaa cctcttcgaa
     2221 tactcccgcg ccatattcac gaccgccgtt gcagatgcca gggcttccag gcacccatgg
     2281 gaaccacagc cacacagcgg accccctatc tgtactatcg tgtggcccag ttcaccaccg
     2341 tagccattct tgccggtcat aagggtcccg tgggttatca ctccgccccc cacacccgtt
     2401 cccagagtca ggccgatcat gtgcttgcaa cccttgaagg caccgaaggc ccactctcca
     2461 agaacaaaag cgttggcgtc gttctctacg aaagttctta taccggtcag ctttttaaga
     2521 atggcggtca attcgaaacc cgaccagtcg ggaagatttg gggagaaaag cacggtaccg
     2581 gtttcgtgat cgatcgaacc gggcgaacca acgccgatac cgacaactcc gtcgacagag
     2641 atcccggtct ccataatcag ctgcttgata gcttccacaa tacgcgtcac taccgctatt
     2701 cttccttcga aaaccttggt gggtattata cttttggaaa gtatccttcc gttctcttcc
     2761 acaatgccta ttttggtctc agtgccacca agatcgactc cgaccaccag atccgacatt
     2821 caaatcaacc tcgttcagta ttgttttttc tccatttcga tcaggtccag gaactcttcg
     2881 ttgctggccg ttcctctcaa tttgtccgtg atcaatctca gtccctcttc ttcggacatg
     2941 gccgacaaca tgcgtctcaa cacccacact ttcctgagaa tgtcctgcgg aagaagcagt
     3001 tcctctttcc tcgtccccga gagcgtcaga ttgatcgcgg ggaacattct cttgttggcc
     3061 agttgcctgg agagaacgag ttccatattt ccagtgcctt tgaactcttc gaagatcact
     3121 tcgtccatct tgcttccggt ctccaccagg gctgttgcga ttatggtgag gcttccgccc
     3181 tcttctatgt tccgggcggc accgaagaag tgcttgggct tgtacagtgc cgacggatcc
     3241 actccaccag agagtagctt cccgctgggg ggaacgtaga ggttgtaagc cctggcgagt
     3301 ctggttatgc tgtccagcag gatcaccacg tcgtaattga actcgaccag cctcttcgcc
     3361 atttctagag tcatttccgc aacttttatc tgtttttcgg gatgcatatc gaacggagcg
     3421 gcaagaacgt gggcgctggt ggttctcttt aggtctgtca cttcttcggg cctttcgtcg
     3481 atcagaagta ttattctgat cgtgtcggga tggttctcgg cgatgccgtt ggccagttct
     3541 ttcaaaagca ccgtctttcc ggctttcgga ggagaaacta tgagacctct ctgacctttt
     3601 ccgattggag aaaagagatc gagcatcctc gtgctcagaa cgtgcgtatc cgattccgac
     3661 attaactgtt cttccggaaa tatgggagtc aggttctgga acgtgatcct ttcggctgcg
     3721 aagttgatgg ctttgtggtt gatcgcttcg attcgtagaa gggcgaagta tttttcccct
     3781 tctttcggcg gtctcacctg accggcaact gtgtcgccgc tggttaggtt gaaccgtctt
     3841 atctgcgatt ggctgacata gatgtcgttc gaactcggca aaagattgtt ctcaagagtt
     3901 ctaagaaaac cgtaaccgcc atcggttatc tccaggactc cctgatcgaa gaagtatccg
     3961 taggattccg tctgagcttt gagaacggcg aagatgagat ctttcttggc catttgagtg
     4021 taattggcga cgtcgtattt tctggccagt ttgtagatct cccttatcgg catgttctga
     4081 agctgagaga tatcgatctc gacctctctg atgttgtctt tgtccttttt tgcctctttc
     4141 agctcctcag gggtttcttg cctgggtccc tcgctgacct tttccgattc ttcggctatt
     4201 tctgccgtct cttcttcggt tgcctttact acttcgagcg gctcctcgac gggttcgatc
     4261 tttgagacct cggaaataat cttctcttct ttggcctcta ctttttcttt gggtttcgag
     4321 gttgttcttc ttctggcggg tttcttttct tccttttcaa ttttctcgat cgggatttct
     4381 tcactcttct tttttcttgg actcaaatca agcggtacac tcaacctacc attcagtaag
     4441 aatgtaccta tcctccttcc gtgtagtagg gattcagtgt tatttgaagc tattgaacaa
     4501 aagaaagcat gggagaccta cctccggtca accacccgcg accgaagtcg agggtttgca
     4561 gatcgaacgc gctcaggggc cagttgacga cagtccatcg gccggtgtat ccagacaaat
     4621 gatgggtaac gactgcggtg ccgtttttgc cttacagatt tcgatccgtt aaccaaagag
     4681 ttcgtgccct gatattacat aggtggtcac ggcaccgtat caacggtttt cacaaatcat
     4741 atcaaatatt cgaaggtaga ggatcggcaa ttcctcccct ggatctgtcc aggatcttcc
     4801 ttgccgaaat tatcgtgaga aacatcagta tccgtcagca ataatatacc atttaagaag
     4861 cagaaaacaa agcttatggg cgttgctttc tttcgtattc ctgccagtca ctctggaatc
     4921 ttttcaaacc gttgtcggtc aacggatggt tgaaaagcct caacaaaaca tcgtaaggaa
     4981 tggtcacgat gtctgcgcct atcaaagcgg cttgaagaac atgcatcgga tgtctcacgc
     5041 ttgccactat tatctccgtt gcaaaaccgt agttaccgaa gatctcatcc atctcgctca
     5101 ccagttgcat tccgtcgttc ccaacatcat caagtcttcc cacgaagggg ctgacgtagg
     5161 ttgcgccggc tttagccgca agaagtccct gcagcgaact gaagacaagg gtgacgttcg
     5221 tctttattcc ctcttcgctg agaactttca cggcccttat cccgtcgggc gtcatcggga
     5281 ttttgaccac gacatgttcg ttcagagagg ccagctctct tgcctgcgac agcatgttgt
     5341 cgaaatcggt cgctatgact tcggcggaaa cgggaccctt tacggcttca catatctcca
     5401 caatgcgttc ttcgaacttc gcgttttcct tcgaaacaag cgttggattg gtggtgacgc
     5461 cgtccacgag ccccaatttc attccatttc ttatctgttc gatgtttgcc gtatcgagaa
     5521 atatcttcat gtgccacctc gcaaaaaatg gctacctttc ggtagccatc acttcgtctc
     5581 tttcttctcc tcgttgtcca caagctggat gacggccatc tcgctggcgt ctccccttct
     5641 catgcccagt ttaactattc tggtgtatcc gccgtttcgg gtcaagtatt tcacagcaac
     5701 ttcatcgcag atcttcttca cgagccttct gtcgttgaag ttcctgttta tctctcttcg
     5761 aagggcgaga tttctggact tgagttcaat ggcctccggg ctttcggcgc tcttcgtacc
     5821 cagttcctgg gttatcgtag tagccttctt cgccctggtt attatagatt ccacgaacgg
     5881 tctcagctct ttggccttgg tgaccgtggt gactatggta ccgtgctcga aaagctctct
     5941 ggctagattc ttcatcagag acactcgctg gctgtggggc atattcaatt tggatccact
     6001 tactctatga cgcatggcta attgacctcc tcaccatcat tctttcttga gtatgatgtt
     6061 gaacttgtca cgcagtttct tgacaacctc cagcatcgat ttctcaccga agttcctgat
     6121 cttcagtaaa tcggcttccg ttttgtctat cagatctccg atagtgttta tcttatctct
     6181 cttcaggcag ttcagggacc ttaccgagag atcaagctct tcaactcttt tcgaggtgag
     6241 cggattggaa tcgacctctt cagtttgtgg agattcactc gctatctgcg tttcttcctg
     6301 taccagctct tcctcttccg gttcggcggg ggtgactatt gaaacctgat tctgctgacc
     6361 caggccgttc gagacaactt cgaagtgtct ggtcaggata tccgtcgcgc ggagtaaggc
     6421 ctccgacggc ttggtcgact tcttagtcca gacttcaagc acaagcttat cgtagtccgt
     6481 tctctttccc acacgaacgt tctctgtctg aaaattcacc ttcatcaccg gactgaatat
     6541 accatctata tagatcatct caacatcgtg atcgatctcc atctccgaaa ccggtacaaa
     6601 gcccttaccg atttccgcga agagttcgat ataaactatc gaatcagtgt tgagcgttgc
     6661 tatgtactga gaaggattca ccacttctac accggtcggt gtcttgatat ctccggccga
     6721 aacgactccc ggtccgactt tttccagtgt gagtttcacg gggtcggaga actccagagc
     6781 gggtttcagc tgaactttct taaggttgag tattatctcc aggatatcct ctttcacacc
     6841 ctcaatcaca tcgtattcat ggtacttacc cggaatcttg agtttagtta tagccatact
     6901 gggtatcgat gaaagaagca ctctcctcaa cgaattaccg atggtgatcg cgtaacccct
     6961 ctccattgga gagagcacga accttgtgta atagctattt tgatcttcct tttcctcttc
     7021 gactcgcagg ttcttgggca ttatagatgc tatcatagtt ctcgcccagc tttcgggcac
     7081 acacctcctt tcaagaaggt tagaatcggt ttacttggag tagagttcga taatggactg
     7141 tagatcaaca ggaatctcca cttcttctct ggccggatac cttagatagg tacccctgaa
     7201 atcctcgtag ttgatttcca gccatggata tgccgctctt tctttagcgg cttctacggc
     7261 ttccttaacg ggcagtacca ccctgctctt ttccttaatt tccacgatat caccgactct
     7321 taagttgaag gagggcttgt tcactcttct accgttcacc gtaatgtgcc catggctgac
     7381 gagttgcctt gcctgtctcc tgctggaggc aaaccccatt ctgaaaacgg tattgtcgag
     7441 cctcgattca agtatcttca acaaagcgtt acccgtctct tcatgcttcc ttacagccat
     7501 ttcgaagtat cttctgaact gtctttcgag gaccccgtaa atcctcttga caacctgctt
     7561 cgacctgagc tgcattccgt actgcgtcaa tttgcgagta gaagctccgt gctgtccagg
     7621 tgccaccggt ctcttaatct ggccgcacct tggagaaaaa catctctcac cttttagata
     7681 taatttaaaa ccttcacgac gacagagttt acaaacaggt cctgtatatc tggccattca
     7741 attccctcct caattacact cttcttcttc ttcttggtct gcaaccattg tggggcaatg
     7801 gtgtgatatc tttcagtgtt tcgatagtca aacctgccgc ctggatcgtc cttatagctg
     7861 cctcacgacc ggagcccggt cccttaactt cgattccaac cctggtgatg ccgagcttta
     7921 tagcttcttt tgcgattttg tctgcgccaa gctgagcggc atacggagtc gacttcttag
     7981 agcccgaata acccgccgta ccgccggatg tccacatcag tgtattccca tcgggatcgg
     8041 tcagggtgat tatagtgtta ttgaaagagg attttatatg tacaatccct ttgtctgaag
     8101 tgagctttct tcttttcttg gctgctggtc tctttgccat tcgatatacc tcccgctaat
     8161 tttctagctc ttcttacgga tcttgctcga tctggaaccc tttctggttc ttccattcga
     8221 atgagtcttc tgacccctga caggcaaccc gtttctgtgc ctgtaacccc tgtagctgcc
     8281 tatctcgatc agtctcttga tcgagcggtc aacctccgtt ctcaattcac cttcgacctt
     8341 atagtgttcg tttatgaact tcgctatttt actgacttct tcgtcggtca gatccttcgc
     8401 cctcttgtcg ccgtcgattt ccgttccggc aagaatctca ttggctcttg tgtaccctat
     8461 accgtaaata taggtcaggg caacgaaagt ctttttgttg ttaggaagtt caacaccgag
     8521 aatacgtgcc atctattttc ctcctcaacc ctgacgctga ttatgtttgg gatttttttc
     8581 gcagactatc cttattctcc cgttgcgtct tatgatcttg cagtgttcac atctcttctt
     8641 gaccgaagcg cggactttca attcgtttct c
//
LOCUS       DAOOLY010000084         8644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9557_length_8644_cov_5.169053, whole genome shotgun sequence.
ACCESSION   DAOOLY010000084 DAOOLY010000000
VERSION     DAOOLY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_8644_cov_5.169053"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1089)
                     /locus_tag="PLD15_05775"
     CDS             complement(55..1089)
                     /locus_tag="PLD15_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB63447.1"
                     /translation="MAGKTTIKDIAKSLGISPSTVSRALSNGPGVGGDLRKRIRKVAS
                     QLNYIPNSSAKSLRTRKTKTIGLIVSDIRNPFFLEFMGGVEAVLFPREFKFIVCNTGE
                     DSKKEEIYIRWLMEHGVEGILSSPYQDREGSNNGAFYKKAMKSGIHVVFYDRLLEGVG
                     GIDSVTLDNEQAIMNGVFYLRERGHERIALCLHKRGIYTIEERYSGFKKACAILGIHP
                     DDRWVLEDMTDYSVSLDKLRSVLTASDRPTAVISTNQYLNETVVRAARELGLDIPADL
                     SLMGFDDSSLNELIDPPVTTIRQPVVDMGKIAATLLLGRIDGDRGEQTRVVLKAELLE
                     RGSVASPAGK"
     gene            complement(1223..1627)
                     /locus_tag="PLD15_05780"
     CDS             complement(1223..1627)
                     /locus_tag="PLD15_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HPB63448.1"
                     /translation="MTKLAIIKGIESAIENKLKELGIVSVESLLEACATKKARTELAK
                     KTGIPEDKILTWSNHADLIRIRGVGGKYSELLEAVGVDTVPELSKRNPENLYLKMIEV
                     NEKEKLVESLPAKKQIIKWVEQAKKLPRVLQY"
     gene            complement(1712..2287)
                     /locus_tag="PLD15_05785"
     CDS             complement(1712..2287)
                     /locus_tag="PLD15_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPB63449.1"
                     /translation="MGLFDTLSKQLSEKSTAAKLGESVGVSPEKVQKLTEIGLPTLLQ
                     GLLKNSSTPEGAASLAKALDQHKNDNVSSITSFLNGVNKDDGVKMLSHIFSGKEKQVE
                     KSLAKETGLKTNQVAGLLSQLAPLLMGFLGQEKKKENLDASGIAGLLSNVASQGDSGM
                     MGMAANFLDSNKDGNIIDDVGNLFGKIFKKK"
     gene            2795..3511
                     /locus_tag="PLD15_05790"
     CDS             2795..3511
                     /locus_tag="PLD15_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB63450.1"
                     /translation="MIPIYLEIRSYMIKQIEKGRLVPGDKIPSERELVDLFKVSRMTV
                     RHAIDQLVNDGILVRMVGKGTYVSSERLSTALNVLNSFSEDIIASGHSPSAKLISVEK
                     TQTGNTISSLLRIDKSREVIKVERLRLVDGLEMAITTSFFPVSRALPLDELDFTATSI
                     YRQIESLGLRIEKASETVTAEAADKRSAGFLRVKENSPLLKIIRLTFISNNVPIEYME
                     GLFRPDRYVISQELYRQGVR"
     gene            3511..4335
                     /locus_tag="PLD15_05795"
     CDS             3511..4335
                     /locus_tag="PLD15_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HPB63451.1"
                     /translation="MKMFEEMFGKKLPIIGVVHLQPLPGAPRYEGMAMKEISRIAVEE
                     ATIMVENGIDGLIVENFRDMMFKKRVGPETVAAMTYIAFEVASRLSVPIGLCVLQSDA
                     ISALAIAKAVGGKFIRVPYYTETYIVDAGTMESVAADALRFRKMIEAGDVKIFADVHI
                     KHGYSLSRRSIEESAEDAYERGLADAIIVTGKKTGGKTKPEDVEAVRNYLPDVPLIVG
                     SGVTQENLSEYFPKLCDAVIVGTSLKKDGKTEGPIDPERVRQFSKSMEKCRKELDR"
     gene            4332..5150
                     /locus_tag="PLD15_05800"
     CDS             4332..5150
                     /locus_tag="PLD15_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HPB63452.1"
                     /translation="MTTVSEFNEIFATSKPIIGMIHLKPLPGSPVYDGKGLKKVIDQA
                     LEEAGKLIDGGVDGVQVENYNDPSYFPDVAPAETVSSLSIVAHEVHKAYPNVPMGICL
                     LADPIASIAVAHTSGARFVRATVFTEAAVDVSGLALRRPHEILRYRKFLDPSIKIFAD
                     VHIKHSAPLAMRPIEESAYDAAYFMADAVIISGKHTGFETPLEDLKKVRGVLPDYPIM
                     VGSGMNKVNAQKILEVASGAFVGSTFKIDGNSYKSVDCERVKEFMKVIGEIRNR"
     gene            5147..6106
                     /locus_tag="PLD15_05805"
     CDS             5147..6106
                     /locus_tag="PLD15_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPB63453.1"
                     /translation="MKKSVLVLGDINVDIIGRFDGFPVPGGSVYSEKPILNPGGSGLN
                     TFVGLKKLNVDASFETMIGNDLFGDYIKVCLSELGIEFTPKVSSSYPTGVVFSLSAQN
                     ERTFFSFRKNAADIHMTCEDLSQVYESPDLLYLTGVSIVEGAETFHTFLEAVKKAKRS
                     GTKIFFDPNMRKTDPVSIERIEQVIPYVDVFLPAQDELTVLFSKSRKFGFCLDLLDAG
                     ISEIWIKKGAKGCSFISGDFSIDFPASKVKVLDCTGAGDAFNAAVVWGHVNELDEKEI
                     GTYANVYAGMSTERIGAATSYPDRESMLKSEYYKSIKMGVKVL"
     gene            6103..7089
                     /locus_tag="PLD15_05810"
     CDS             6103..7089
                     /locus_tag="PLD15_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB63454.1"
                     /translation="MKRLILVLALLSFVAMGLGALKVAIVAGDAIGDRGFTDMAYIGI
                     QEAQKQFGIEYKVFECNVDPSKYFDSLRAAAMNYDLIFVDPGYFFDKELAEVSAMFPS
                     KTFVYIDGTSSLPNVVSVPFKQNEGAFLAGCLAALLTNKTELCKINAEKIVGFVGGFD
                     MPVIRDYQAGFEHGVKYVDPSVKVIARYAGDHYDPALGKETAYSVFKEGADVIFQAAG
                     PTGLGILEAARDYGFYAIGVDTDQGYLQPGFIVSSMMKKVDVAVLDIVRMQIENSLEK
                     GSVHVYGIANGGVGLAYNQFMMDTVPASVYIEISEIQEKISNGEIVVDSYLD"
     gene            7165..>8644
                     /locus_tag="PLD15_05815"
     CDS             7165..>8644
                     /locus_tag="PLD15_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63455.1"
                     /translation="MSKTYPNGLEALREVSFEVRKGEIHAIVGENGAGKSTLMNILFG
                     LIKPTSGEILFNNNHFSPKHPSEAIDLGIGMVHQHFKLVQSITVAENIVLGMENRFQS
                     KLRRVDREAMNESVASLIDRLRMSISPDDRIDSLPISKRQQVEILKVLYREADFVILD
                     EPTAVLAPNEVEDFLEFVRGIREMGKTVIFISHRLGEVFSLADRITILRRGNVIGTFD
                     KRDVDRESVANMMIGTATDLSEDPPDCLGNESVLSVEGISDVSGTIRDLSFQLREGEI
                     LGFAGVGGNGQEELFEALAGSLRLASGDIRLVGSSISSLDTLERRRAGLALITDDRMG
                     KGLAPDRSIVENCMAGHHKFGKFGKWLIKDTDARSFTESIVADYDVRCGKNIKTAVRA
                     LSGGNMQKLLVGREIAYEPVVLVAAQPTAGVDVAAKKLIHDSFRRLVKTGSGVILISG
                     DLEEIMGLANRIIVLYRGRAVTEMTHPHYDPTEISYYMTGIRG"
BASE COUNT         2223 a         2083 c         2218 g         2120 t
ORIGIN      
        1 catactcctc ctccttttat gccttaaaca gtggcgatca tcataaccgt tttactattt
       61 tcctgccgga gaggccacag aacccctttc cagtagctcg gccttcaaca cgaccctggt
      121 ctgctcccca cggtccccgt ctatcctgcc gagaagtaat gtggcggcta tcttgcccat
      181 atccacgaca ggctgtctga tagtcgtcac cggtggatct atgagctcat tcagagatga
      241 atcgtcaaag cccataaggg aaagatcggc cggtatatcc aggccgagtt cgcgggccgc
      301 cctgactacc gtctcattca ggtactggtt ggtggagatc accgccgtcg gtcgatcgga
      361 ggccgtcaat accgacctca gcttatctag cgagacggaa taatccgtca tatcctccag
      421 aacccacctg tcgtcgggat gtattcccag aatcgcacag gcctttttga atcccgagta
      481 tctctcttct atcgtataga tccctctttt atgaagacag agcgcaattc tttcgtggcc
      541 acgctctctc agatagaaaa caccgttcat tatcgcctgc tcgttgtcaa gcgtcacgga
      601 gtctatgccg ccaacccctt cgagaagcct gtcgtagaag accacgtgga ttcccgactt
      661 catagccttt ttgtaaaagg cgccgttgtt gcttccctcc ctatcctggt aaggggaaga
      721 gaggatgcct tccactccgt gttccatgag ccatcttatg tatatctctt cttttttcga
      781 atcctcaccc gtattacaca ctatgaattt gaattcccgt ggaaagagaa ccgcctccac
      841 tccgcccatg aactccagga agaagggatt tcttatatcg gagacgatga ggccgatagt
      901 cttggttttt cttgttctca gggattttgc ggatgaattg gggatgtaat tcagctgtga
      961 agcaactttg cggatccttt tacggagatc gccgccgacg cccggtccgt tcgacagagc
     1021 tctcgacacg gtcgaaggcg atattccgag actcttcgcg atgtctttga tcgtagtttt
     1081 tccggccaca acaaacctcc gcaaacgttt gcgtacaatt gcattataca atcaacctct
     1141 gagaaaaggt aggacccaga ggtcctaccc ttcgtgcttg gattgccata aactgtcagg
     1201 aagatcgaat cgccgaaccg cttcagtact gaagaactcg aggtaatttc ttggcctgtt
     1261 ccacccattt tatgatctgc tttttagccg ggaggctttc aaccagtttc tccttttcgt
     1321 taacctcgat catcttcaaa tacaggttct cggggtttcg cttcgacagt tccggtacgg
     1381 tatcgactcc tacggcttcc aggagttcgg aatacttccc gccaactccg cggattctga
     1441 tcaagtctgc atgattggac caggtaagaa tcttatcttc cggaattccg gtcttctttg
     1501 cgagttccgt tcgggccttt ttcgttgcgc aagcttccag aagagattct actgaaacta
     1561 tgcccagctc tttcaactta ttctcgatcg cggattcgat tcccttaatt atagccagtt
     1621 ttgtcacaga ctctcctcct ctcttaaaat gtattgaaca gacagatcca gattttatta
     1681 gtcgaccagc cgttagctat ttgtgcttaa attatttctt cttgaatatc tttccgaaca
     1741 ggtttcccac gtcgtcgata atgtttccgt ctttgttgct gtcaaggaag ttggccgcca
     1801 ttcccatcat tccgctatct ccctggctgg caacgttcga caacaaaccg gctattccag
     1861 aagcgtccag gttctctttc ttcttttcct ggccgaggaa acccatcaga agcggtgcca
     1921 gctgcgacag aaggccggca acctgattcg ttttcaagcc ggtctctttt gcgagacttt
     1981 tctcaacctg tttttccttg ccggaaaata tgtgactgag catttttaca ccgtcatctt
     2041 tatttactcc gttcagaaaa ctggtaatgc tgctgacatt gtcattcttg tgctgatcca
     2101 aagcctttgc cagcgaagca gccccttcag gagtggagga gttttttagc agcccctgaa
     2161 gaagtgtcgg taatccaatc tctgtgagtt tttgtacctt ttccgggctt acaccaacag
     2221 actcgcctaa cttcgctgcc gtgctttttt cgctgagctg cttagaaagc gtatcgaaaa
     2281 gacccatttt tttcctcctt ttaaaaataa gtaacatcaa acttattata cttctttatt
     2341 tataattgcc ctagctaatg agagagactc gaaaaagccg ggatctgaaa ccttaaggcg
     2401 atcagcggtt gacacagtat aagccccgag cgtgggttcc tggtaaaaca gaggccatag
     2461 agagggaggg agagactgaa gagatgaggt cccgtatagg tttcctgaat atgaacattt
     2521 cagggcaggc tcatcaggac aggctccacg gaatgacgac gcgaaaaacc ctcgacgctc
     2581 tttctcgtgt ttttaccccc acctctccac gcgcacccaa caaccgttct aagtggtata
     2641 agtccgcaac ttaccagttg aagtgtgttg gggttagctg tctaaacacc tctttgaaag
     2701 cttaattaca actgaagcat tggttaaaaa aagaagtata gaattcaggt ggtatagaca
     2761 tatagatatc tatacgcgat tgcgaggtgt cggcatgata cctatctatc tggaaatcag
     2821 aagctatatg attaaacaga tcgaaaaagg gagactcgtt cccggcgaca aaatcccatc
     2881 cgaacgagag cttgtcgatc tgttcaaggt aagccgcatg accgtgagac acgcgatcga
     2941 tcagcttgtc aacgatggaa tccttgtcag aatggtcggc aagggtactt atgtgagcag
     3001 cgagagactt tccacagcac tcaatgttct taacagcttt tcagaggata taatcgcttc
     3061 cggccacagt ccctccgcaa aacttatcag cgttgaaaag acccagaccg gcaatacgat
     3121 ctcttccctt ttgagaatcg ataagagccg ggaagtaata aaagtcgaga ggctaagact
     3181 ggtggacggt ctggaaatgg ccataaccac atccttcttc cccgtttcaa gagcgctacc
     3241 ccttgacgaa ctggatttca cggcaacttc gatttacagg cagatagagt ctctgggtct
     3301 gaggatagaa aaggccagcg aaaccgttac cgccgaagcg gccgacaaga gatcggccgg
     3361 ctttctgagg gtaaaggaaa actccccgct gctcaaaatc ataagactta catttatctc
     3421 aaacaacgtt cctatcgaat acatggaagg attgttcaga ccggatagat atgtgatatc
     3481 ccaggaatta tacagacagg gagtgagatg atgaaaatgt ttgaagagat gttcggaaaa
     3541 aagctgccga taatcggtgt cgttcatctg cagccactgc cgggagcgcc aagatacgaa
     3601 ggtatggcga tgaaggagat ctccagaata gccgtcgaag aagctaccat catggtcgaa
     3661 aacggaatag acggcctgat agtcgaaaac ttccgggaca tgatgttcaa gaagagggtg
     3721 ggaccggaaa ccgttgcggc gatgacttac attgcctttg aagttgcgtc cagactttcc
     3781 gtgccgatcg gactatgtgt ccttcaatct gacgcgattt ccgctctcgc gatagcaaag
     3841 gccgtgggtg gaaaattcat cagagtccct tactacaccg agacttatat tgtcgatgcc
     3901 ggaacgatgg agagcgtggc ggcagatgcc ctcaggttca gaaagatgat cgaggccgga
     3961 gatgtgaaga tatttgccga cgttcatatc aaacacggct attctctgtc gcgaagatcg
     4021 atcgaggaat cggccgagga tgcttacgaa cgcggactcg ccgacgcgat tatcgtaacg
     4081 ggaaagaaga cgggtggaaa gacaaagccg gaagatgtgg aggcggtcag gaattatctt
     4141 ccagatgtcc ctctcattgt cgggagcggc gtaactcaag aaaacctctc cgagtacttc
     4201 ccgaaactct gcgatgcagt aattgtcgga accagcctta agaaagacgg aaaaaccgaa
     4261 ggtcccatag atccagagag agtgaggcag ttctcaaaaa gcatggaaaa gtgcagaaag
     4321 gagctcgacc gatgacaact gtatctgaat tcaacgaaat cttcgctact tcaaagccga
     4381 taattggcat gattcacctc aagccgctgc cgggttcgcc cgtttacgac ggaaagggtc
     4441 tgaagaaagt tatagatcag gcgctcgaag aggcggggaa gctcatagac ggaggagtcg
     4501 acggcgttca ggtggagaac tacaacgatc catcgtattt tcccgacgtc gctccggccg
     4561 aaaccgtctc ctcgctttcc atagtcgccc acgaggttca caaagcctat ccgaatgttc
     4621 cgatgggaat atgccttctt gcagatccga tagcgtccat agcggtagct cacacctcgg
     4681 gtgccagatt cgtcagagcc acagttttca ccgaagcggc ggtcgacgta tccggtctgg
     4741 cattaaggag acctcatgag atcctaagat atcgcaagtt cctcgaccca tccatcaaga
     4801 tcttcgcgga cgtccatata aaacactcag cgccgcttgc gatgagaccg atagaggaat
     4861 cggcttacga cgcggcgtat ttcatggccg atgccgtgat cataagcggt aaacacacgg
     4921 ggtttgaaac acctctcgaa gatctgaaga aagtgcgcgg ggtacttccc gactatccga
     4981 taatggtggg atcgggtatg aacaaagtca acgcgcaaaa gatactcgaa gtcgcctccg
     5041 gcgcctttgt cggctctaca ttcaagatcg acggtaactc ctacaaatcc gtggattgcg
     5101 aaagggtgaa ggagttcatg aaagtgatcg gggagatcag gaatagatga agaaatccgt
     5161 tctcgtgctg ggcgacataa atgtggatat aatcgggagg ttcgacggct tccccgtgcc
     5221 tggaggctct gtctatagtg aaaaaccgat cctgaatccc ggtggctcgg ggctgaacac
     5281 atttgtgggt ctgaagaagc tcaacgttga cgcatcgttc gagacgatga taggaaacga
     5341 tctcttcggt gattatataa aggtttgcct ttcggagctg gggattgagt tcactccgaa
     5401 ggtttctagc tcgtacccga ccggagtggt tttttcgctt tcggcccaga acgagcgaac
     5461 tttcttctcg ttcagaaaaa atgccgcaga catacatatg acttgcgagg acctctcgca
     5521 ggtttacgaa agcccggatt tgctttatct caccggtgta tcgatagtcg aaggagccga
     5581 gacctttcat acgttcctgg aagcggtaaa gaaggcaaag cgttcaggaa cgaaaatttt
     5641 cttcgatcca aacatgagga agaccgaccc ggtttccatc gagagaatag agcaagtgat
     5701 tccttacgta gatgtcttcc tcccggctca ggatgaactg accgttctat tctcgaaatc
     5761 gaggaaattc ggattttgtc tcgaccttct cgatgcgggg atttccgaga tatggatcaa
     5821 gaaaggcgcc aaaggctgtt cttttatttc cggagacttc agtatagatt ttccggcttc
     5881 taaggtcaag gttctcgact gcacgggcgc tggcgatgct ttcaacgcgg ccgttgtctg
     5941 gggacacgtc aatgaactgg atgagaagga gataggaact tacgcaaatg tctacgcggg
     6001 aatgagcacc gagaggatcg gggcggcgac ttcttatcct gacagggaaa gtatgttgaa
     6061 aagcgagtac tacaaatcta tcaagatggg ggtgaaagtc ttatgaagcg tttgattcta
     6121 gttcttgcgt tactgagttt tgtagcgatg ggtttggggg ccttaaaggt tgccatcgtt
     6181 gccggcgacg ccattggaga ccgcggattt acagatatgg cgtacatcgg cattcaggaa
     6241 gcccagaaac agttcggaat cgagtacaag gtcttcgagt gcaatgtaga tccttcgaag
     6301 tatttcgatt cgctgagggc tgccgccatg aattacgatc tgatcttcgt ggacccgggt
     6361 tatttcttcg ataaggaact cgccgaagtc agcgcaatgt tcccctccaa gacttttgtc
     6421 tatatcgatg gcacatccag ccttccgaat gttgtctccg tgccattcaa gcagaacgaa
     6481 ggcgctttcc ttgccggttg cctcgcggcc ttgctcacga ataaaaccga actctgcaag
     6541 ataaacgcgg agaagatcgt gggattcgtc ggtggcttcg atatgccggt aataagggat
     6601 tatcaggccg gtttcgagca tggagtgaag tatgtagatc caagtgtcaa ggttatagcc
     6661 agatacgccg gtgaccacta cgacccggca ctcggaaagg aaacggccta ctctgtcttc
     6721 aaagagggag ccgacgtgat attccaggct gcgggaccga cgggactggg aatactcgaa
     6781 gcggccagag attacggctt ctacgcgata ggcgtcgata ccgatcaggg ctatctccag
     6841 cctggcttca tagtctccag tatgatgaag aaagtggatg tcgcagttct tgacatcgta
     6901 agaatgcaga tcgaaaacag cctcgagaag ggttccgttc atgtatacgg catcgcaaac
     6961 ggcggcgtcg gactcgcata caaccaattc atgatggaca ctgtgccggc atccgtctat
     7021 atagaaatca gcgagataca ggagaagatc tcgaacggag agatagttgt agattcatat
     7081 ctcgattaaa gattccttcc ggagccccta taggggctcc gggtttcata tcggggggtg
     7141 ttccaaattc tagtctgcaa aagagtgtcg aaaacatacc cgaacggtct ggaagctctc
     7201 agggaagtga gtttcgaggt tagaaaaggc gaaatacacg cgatagtcgg tgaaaacggc
     7261 gcgggaaaat ccactttgat gaacattcta ttcggtctga ttaagccgac ttcgggagag
     7321 atcctattca acaacaatca tttttcgcca aaacaccctt ccgaggcgat agatctggga
     7381 ataggaatgg ttcaccagca cttcaagctt gtacagagta tcaccgtggc cgaaaacatt
     7441 gtacttggaa tggagaaccg tttccagagc aagttgcgca gagtcgacag agaggcgatg
     7501 aacgagtcgg tcgcctcgct catcgacagg ctcagaatgt ccatctcgcc cgatgataga
     7561 atcgactctc ttcctatcag taaaagacag caggttgaga tcctcaaggt tctgtacaga
     7621 gaagctgatt tcgtaatact cgacgaaccg acggccgttc ttgcgcccaa cgaggtggaa
     7681 gatttccttg agtttgtgag gggaatacgc gaaatgggaa agactgttat cttcatttcc
     7741 catagattgg gagaggtctt ctcccttgcc gacagaataa cgattctgag aaggggaaat
     7801 gttattggaa cctttgacaa aagggatgtt gacagagaga gcgtggcaaa catgatgatc
     7861 gggaccgcca cggatctctc cgaggatccg ccggactgcc tcggaaacga gagcgttctt
     7921 tcggtcgagg gaatttccga tgtatccggt actataaggg atttgagctt ccagttgcgg
     7981 gagggagaga ttctcggctt tgcgggagtt gggggaaatg gtcaggaaga actcttcgaa
     8041 gctcttgcgg ggagtctgag attggcctcg ggggatataa ggctcgtagg ttcatctata
     8101 tcttctctcg acacgctcga aagaagacga gccggtctcg ccttaatcac cgacgaccga
     8161 atgggaaagg ggctggctcc cgatagaagc atagtggaga actgcatggc cggccaccat
     8221 aaattcggaa aattcgggaa atggttgata aaggacaccg atgcaaggtc tttcacagaa
     8281 tcgatcgttg ccgactacga cgtgcgctgc ggcaagaata tcaaaacggc cgtcagggct
     8341 ctctcgggcg gaaacatgca gaaactgcta gtcggtcggg agatcgctta cgaaccggtg
     8401 gttctcgtcg cggctcaacc cacggcaggg gtcgacgttg ccgcgaaaaa gctcattcat
     8461 gattcattca ggaggctggt caagacaggc tcgggagtaa tcctcataag cggcgatcta
     8521 gaggagataa tgggtcttgc gaacagaatt atcgtgctct atcgagggag agccgtcacc
     8581 gagatgacgc acccccatta tgatccgacg gagataagtt actatatgac ggggataagg
     8641 gggg
//
LOCUS       DAOOLY010000085         8634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9572_length_8634_cov_4.969693, whole genome shotgun sequence.
ACCESSION   DAOOLY010000085 DAOOLY010000000
VERSION     DAOOLY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9572_length_8634_cov_4.969693"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLD15_05820"
     CDS             complement(<1..611)
                     /locus_tag="PLD15_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HPB63456.1"
                     /translation="MVQPESSADLKKTTIALLGNPNVGKTSIFNCLTGSRQVVANWPG
                     VTVEKRVGSLRHGDLTLDIVDLPGTYTLGSRSLDEKIARDFLIDERPNVVVVIGDAIN
                     LERSLYLLLQVLELRGDVVLAVNAVDEARKAGLEIDKNELGKQLGIPVVITSAATGEG
                     IEELKEVLVKSARSSSHIHYLFSEEIEEKIEALAGRFRLVPALS"
     gene            complement(620..1066)
                     /locus_tag="PLD15_05825"
     CDS             complement(620..1066)
                     /locus_tag="PLD15_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPB63457.1"
                     /translation="MVVPLSRMCEGEKGKISRLELPVEVRERLCGLGFVNGEEVELVK
                     IAPFGDPKVFRIKGTNVTLRNDIAMWILVETSSIPLSSAQPGEYLVSLINGGWGLKEK
                     MRQYSIQEGATVKVVENSIRRIEVEVHGKTLFLSRGQAFRVIVRER"
     gene            1247..2050
                     /locus_tag="PLD15_05830"
     CDS             1247..2050
                     /locus_tag="PLD15_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPB63458.1"
                     /translation="MDAIYQVDSFTKLPFRGNPAGVYISDMEHDYETCIKIAREMNLS
                     ETAFPVRTTDDNHYKLRWFTPATEVPMCGHATLATSWVYFTELGVDGDIYYETLSGQL
                     KVSRFGDDLKMDFPSELPLRIDCDGPVGEAVGCDGTTYYATVTGKLLFEIAGREKLER
                     LNPDFSMLEKLSVGVPARGLIVTCKGGGEFDFFSRYFAPWVGINEDPVTGSAHTVLGP
                     FWSERLGKDKLRAYQASQRGGEVRLELLDGNRISIIGNAVTVIRGHMSI"
     gene            complement(2055..2798)
                     /locus_tag="PLD15_05835"
     CDS             complement(2055..2798)
                     /locus_tag="PLD15_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63459.1"
                     /translation="MKSISRNVLFYFIALWIFIYILSTTIAGVFPPEVPGYFRIGPWI
                     NPAWILVILTQFIFARTLDKREFDRFYLLSLYVSGALWILFSTFGVESVSLVLAIAFL
                     FLSWQVYRVFRPVYGKLSRQFRDMSIGSLLFSWSIIVLLSSLAMFWRSELTAVFFLFS
                     MVLISSILAHTRADPINSLVFSCFAFSTGIENLSQSYHVAVAGISSGTIFLMIFLFAV
                     MIAREDPAKRVFAAVNPPREEEIKLKINR"
     gene            complement(2855..4585)
                     /locus_tag="PLD15_05840"
     CDS             complement(2855..4585)
                     /locus_tag="PLD15_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPB63460.1"
                     /translation="MDYNQRFQKWLESRYIDDETRNELLSIKNSPEEIKDRFYTQLQF
                     GTAGLRGKIGAGDNRMNRYNIARVTQGLANYIASYGESAQKRGVVIAYDVRHHSREFA
                     EAAASVLCANGIKVFLFDDIRPTPELSFAVIHLSTMSGIMITASHNPKEYNGYKLYWQ
                     DGAQVLPEIADGVYREIEKVALFSGVRYVSLEKAKSEGFLVYTGKEIDDLYISKVKSL
                     SLRDSPEELDKDIRIVFTPLNGTGNGPIRRVLEERGFKKVFVVPEQENPDPDFTTVGY
                     PNPEDVKAFKLAVEMGEKVEADVLLAADPDADRLAVMVRHSSGYVSLNGNQTGALLVN
                     YILLSMEEKGVLPEKGFIVKSIVTGDLAKTIAENYGVSTYETLTGFKNICGKALEIEK
                     SREGNFIFGYEESIGYVTGIFVRDKDAVSSSMLFCEMAAYFLRRGLSIYEVLEGLFER
                     YGFFAEKQISLVLEGVHGQKRIERMMKEYRKDYPLSLDEVDLVSYIDYLEGIETLLRG
                     GGKRSIDIPRSDVVKYLFSDGSWYAVRPSGTEPKLKLYIYSRGKTREATERRIEVFER
                     EIMSRLNGIK"
     gene            complement(4649..6022)
                     /locus_tag="PLD15_05845"
     CDS             complement(4649..6022)
                     /locus_tag="PLD15_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63461.1"
                     /translation="MNELDTEKLHKKTRRLSFMDTFFYNGFFIITQGFILTGLALEYR
                     ADELVISVIGVLPVLSQLVQLLVPFIMNRTGTRKRALLSTALISRITVALISITLATG
                     MVHQSILLILLSVIAICNSFASNFWVSIMKDIVPANISGRFYSRRNLLSSFTAMSMTF
                     IYSSILDNVPGRKGFIIVSIIAAGFALIDFLVLALHHVPPRQEPSYSVGVFLRPLKDL
                     QFRKFISFSFVWNFALAISSPFFSYHQIINLKLDYSFMSIMTIVNSLSLMVFYLLWGK
                     ITDEIGGFDVVHFTLGITIFLPLTWVFTNPGTTFLIPINQVVSGFAWSGVNLTLFTTM
                     MALFPGERAEAYFAVLSFANGLGALLGSLLGGVLATLMKNIKLDLFGFPFYGIQLLFV
                     LSSLFRFLAWTLLKRIRVGKKSSIPRIIYNITAASANRSVARISEFPSIYAAIKGKVA
                     KKIARGD"
     gene            6278..7561
                     /locus_tag="PLD15_05850"
     CDS             6278..7561
                     /locus_tag="PLD15_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPB63462.1"
                     /translation="MLYRKIAIGENLESLPCEEHIQSLFDVDFDFLVNLGKETPTILF
                     IDGGNYSKDILDDWIFAFTNIILINNNPIRAFIIRGYSEETQEFKGYYRVVGSYKSPR
                     QIVQEIIDSIDRSLEKIIGNSDRVIQMKRMIVLSMFYDGSIMILGETGSGKNLLAETI
                     ARLSPRGNKPFYSINCAAIPETLLESELFGYKKGAFTGATGEKIGLIEQADNGTLFLD
                     EIGDMPLNLQAKILAITENREFFKIGATKPTKVDVRFITATNRTEDSAMRNDLRYRLS
                     AVKIDLPPLRERKMDIPLIFDSVLERKGYLVRFENIPRELKERFLSYNYPGNIRELLN
                     MVEEYLALNDVVQATVDSSISKNIKILAEEALVARVVKGTTYKDFLQEIYKGVSREIL
                     IQRAGVLDNDINALAGEFGLTPRRIRDLLSELEAQ"
     gene            7661..>8634
                     /locus_tag="PLD15_05855"
     CDS             7661..>8634
                     /locus_tag="PLD15_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPB63463.1"
                     /translation="MAVRKLNLPQEIEVAELFGEYDRKAKFIQKKLNVKISIIGNEIW
                     IKGEDGNGIEVAKNIVDELLQMTKDGHFVEWREFEYIVDQHLQHDEKDEEFKGVYSEV
                     RETSLLSNRIRPKTLGQKEYLETMKEKEMVFSIGPAGTGKTYLAVAMAVDFLKAGKVQ
                     RIILTRPAVEAGEKLGYLPGTLLDKVDPYLRPLYDALMEMIPTEKLGYYRENSIVEVV
                     PLAYMRGRTLNNSFIILDEAQNTTYQQMKMFLTRIGFNSRVVVTGDITQIDLPRDTNS
                     GLVVIQKVLGGIESIGFSYLTDQDVVRNPLVKEIIKAYDTYERDKQAK"
BASE COUNT         2354 a         2077 c         2052 g         2151 t
ORIGIN      
        1 gaaagggccg gtacgagtct gaacctgccg gcaagggctt cgatcttttc ctctatctct
       61 tcggagaaga ggtaatgaat gtggctggaa gaacgtgcac tcttgaccag aacctctttc
      121 agttcctcta taccttcacc ggtagctgcc gaagttatta ccacaggtat tcccagttgt
      181 ttaccgagtt catttttgtc tatctccagg ccggcctttc ttgcttcgtc aacggcattc
      241 acggcaagca ccacgtctcc cctgagctca agaacctgca gaagaagata tagactcctt
      301 tcgaggttga tcgcatcccc tatcacaaca accacgtttg gacgctcatc gatcaggaaa
      361 tctctcgcga tcttctcgtc gagactcctg gagcccagcg tatatgtacc cgggagatcg
      421 actatatcta gagtcagatc gccgtgcctg agagaaccaa cacgtttttc aaccgttacg
      481 cccggccagt tggcaactac ctgtcttgaa cctgtaagac agttgaatat gcttgttttg
      541 cctacgttgg gatttcccag tagcgctatt gtcgtttttt tgagatctgc tgagctttcc
      601 ggctgaacga ttacgatttt tatctctccc tgactatcac tctgaaggcc tgtcctctcg
      661 aaagaaacaa ggtctttcca tgaacctcta cttctatcct cctgatggag ttctccacga
      721 ccttaacagt agcaccttcc tggatcgaat actgtctcat tttctctttc aatccccagc
      781 caccgttgat gagactcact aagtattctc ccggctgggc gctcgacaga ggtatagatg
      841 atgtttccac caggatccac atggcaatat cgttcctcag cgttacgttg gttcccttta
      901 tcctgaagac tttggggtcg ccaaaaggag cgatctttac cagttcgact tcctctccat
      961 ttacaaaacc cagaccacac agcctctccc tgacttctac cggaagctca agtctgctga
     1021 tctttccctt ttctccttca cacattcttg acagcgggac taccatgggc atcatcctta
     1081 ttgagaatac gtctcaataa gattttacca tctatctcgc ggcttttcag caggtatcca
     1141 aaaaattctc gaaagacaca cgaacttggc agagaaagcg cctgtttttc ccgccagaac
     1201 cgggaaagta gtaacatatc aatatagaag cgacggaggt gtggtcatgg atgcgatata
     1261 ccaggtggat tccttcacaa aactcccttt caggggcaac cccgcaggag tttacatcag
     1321 cgacatggaa cacgactacg aaacctgcat caaaattgcc agagagatga atctctccga
     1381 gacggccttc ccggtgagga cgaccgatga taatcactac aaattgcgat ggttcacccc
     1441 ggcaacggag gtcccgatgt gcggacatgc aaccctggca acatcatggg tctatttcac
     1501 cgagctcggt gtcgatggag acatatatta cgagactttg agcgggcagt tgaaagtgtc
     1561 tcgcttcgga gacgatctga agatggactt tccgtccgag cttcctctca gaatcgattg
     1621 tgacggaccg gtgggagagg ccgtcggttg cgacgggaca acgtattatg caaccgtcac
     1681 cggaaaactc ctcttcgaga tagccggtcg cgaaaagttg gagagactga atccggattt
     1741 ctccatgctg gagaaactgt cggtcggtgt tccagcaagg ggcttgatag tcacctgcaa
     1801 aggcggcgga gaattcgatt tcttctccag atatttcgct ccctgggtgg gaataaacga
     1861 agatcccgtg acgggctcgg cgcacacagt cctcggccct ttctggtcgg agagactcgg
     1921 caaagataaa ctccgggctt accaggcttc gcaaaggggt ggtgaagtaa ggctggagct
     1981 tctcgatggt aacaggatat ccataatcgg aaatgccgtg accgttatca gagggcatat
     2041 gtccatctga tgggttagcg atttatcttt agcttgatct cttcctctct gggagggttt
     2101 acggccgcaa agaccctctt ggcaggatct tccctggcta tcataacggc gaacaggaag
     2161 atcatgagga aaatagttcc agacgagatg ccggccaccg cgacgtggta ggattgcgaa
     2221 agattttcta ttcccgtgga gaaggcgaag caggaaaaga ccagactgtt gatcggatcg
     2281 gctctggtgt gggccaggat cgaggagatc agaaccatcg agaaaagaaa gaatacggct
     2341 gtaagttcgg atctccagaa catcgccaga ctggaaagaa gtacaataat gctccacgag
     2401 aaaagcaaac ttccgatcga catatctctg aactgccttg agagctttcc atagaccggt
     2461 cggaaaactc tgtagacctg ccacgagaga aagaggaacg ctatggcaag caccagtgat
     2521 accgactcca cgccaaaggt tgaaaaaagt atccacaatg caccgctcac gtacagagag
     2581 aggagataga acctgtcgaa ctctctttta tcaagggttc tggcaaagat gaactgggta
     2641 agaataacga gaatccaggc cggattgatc catggaccta tccggaaata gccaggaact
     2701 tcgggaggaa agactcccgc gatcgtggtg ctgagtatgt agataaatat ccagagcgct
     2761 atgaagtaaa acagtacgtt cctggatata ctcttcaaag gacaccctcc gtttccttat
     2821 ttgatttata gaaccagact tcacagcgag agagtcactt tatcccgttg agcctgctca
     2881 ttatctcacg ttcgaatacc tcgattcttc tttccgtcgc ttctctggtc tttcctctag
     2941 agtatatgta caacttcagc ttcggctcgg taccggatgg ccttacggcg taccatgatc
     3001 catcactgaa gagatatttc acgacatccg agcgcgggat atctatagac cttttcccac
     3061 cgcctctcaa aagcgtttct atcccttcga gataatcgat gtacgaaacc aggtcgactt
     3121 cgtcaaggct tagaggataa tccttcctgt attctttcat cattctttct atcctcttct
     3181 ggccgtgtac gccttcaaga accagcgaga tctgcttttc cgcgaaaaat ccataccttt
     3241 cgaacagccc ctcaagaact tcataaatcg aaagtccccg tcttaaaaaa taagccgcca
     3301 tttcgcagaa caacattgaa gaggaaacgg cgtccttgtc tctgacgaaa atcccggtca
     3361 cgtaacctat actctcttcg taaccgaaaa tgaaattccc ctcccgactt ttttctatct
     3421 ccagcgcctt tccgcagatg tttttgaagc cggttaacgt ttcataggta ctcaccccgt
     3481 aattttccgc gatagttttc gctagatctc cggtgactat cgactttacg atgaaccctt
     3541 tttccggcaa gacccctttc tcttccatag acagaagaat gtagtttacc agcaaagcgc
     3601 ctgtctggtt accgttaaga gaaacgtatc cgctcgagtg tcttaccata acggccagac
     3661 gatcggcgtc gggatcggcg gcgagcagaa catcggcttc taccttctca cccatctcca
     3721 cggcgagctt gaaggccttg acgtcctcgg gattcggata accgacagtt gtgaagtcgg
     3781 gatcggggtt ttcctgctcg ggaaccacga aaaccttctt gaagcccctc tcttcgagca
     3841 ctctccgtat cggtccgttt cccgttccat taagcggggt gaagactatt cttatgtctt
     3901 tatcgagttc ttcgggtgaa tctctcaagg agagtgattt caccttgctt atgtacagat
     3961 cgtctatctc ttttccagta tatacaagaa atccctccga ctttgccttt tcgagggaga
     4021 cataacgaac acccgagaaa agagcgactt tctcgatttc tctgtaaact ccatcggcta
     4081 tctccggtag tacctgggcc ccgtcttgcc agtagagctt gtatccgttg tactctttgg
     4141 gattgtggct ggccgttatc atgattccag acatcgttga aagatgaatt acggcgaagg
     4201 agagttcagg agtgggccgt atatcgtcga agagaaagac ctttataccg tttgcacaga
     4261 gcaccgaggc cgcggcttcg gcgaattcac gcgagtggtg tctgacgtca taggctatta
     4321 caacacctct cttttgtgcc gattcaccgt aagatgctat gtaattggcc agtccctgtg
     4381 tgacacgggc tatgttgtat ctgttcatcc ggttgtcacc cgcgccgatc ttacccctca
     4441 agccggctgt cccgaattgc aattgcgtat agaacctgtc tttaatttct tcgggagaat
     4501 tttttatcga taggagctcg tttctcgtct catcatcgat atacctactt tccagccatt
     4561 tctgaaatct ctgattgtag tccatgcttt tccccctctc gataacgatt atatcttatg
     4621 gtgccgctcg aaaacatact ggacggtttt agtcgcctct ggctatcttc ttcgcaactt
     4681 taccctttat tgccgcataa atcgagggaa attcagatat cctcgccaca gacctgttgg
     4741 cgctggctgc ggtgatattg tagattatcc tcggtatgga actctttttg ccaactctga
     4801 ttcttttcag aagagtccat gccagaaaac ggaagagcga agagagaacg aacagcagtt
     4861 gaattccgta gaaagggaat ccgaagagat cgagttttat gttcttcatt aaagttgcta
     4921 gcaccccacc cagaagcgat cccaatagag cgccgagacc gttggcgaaa gagagaaccg
     4981 cgaagtacgc ctctgcacgc tcgcccggaa aaagcgccat catcgtcgtg aagagagtga
     5041 gattcacacc gctccaggca aaaccgctca ccacctggtt gatgggaatc aaaaaagtcg
     5101 ttccgggatt ggtaaaaacc catgtcaggg gcaaaaagat cgttattccc agagtgaagt
     5161 gtacgacatc gaaaccgcct atttcatcgg ttattttacc ccacagcaag tagaagacca
     5221 tgagcgacag actgttcacg atagtcatga tgctcatgaa agagtagtcc agtttaaggt
     5281 ttatgatctg gtgataggaa aagaaggggc tggatatggc cagggcgaag ttccatacga
     5341 aagaaaagga aatgaatttc ctgaactgaa gatccttcaa cggcctcaag aaaacaccga
     5401 cgctgtaaga aggctcctgt cgcggcggta cgtgatggag agccagtacc agaaaatcga
     5461 taagcgcgaa tccggccgct ataatcgata caatgatgaa tcccttacgt cctggaacat
     5521 tgtccagaat agaggagtag atgaaagtca tcgacatggc cgtgaaagaa ctcaagagat
     5581 ttctcctgct gtaaaacctc ccgctgatat tcgccggaac tatatctttc attatcgata
     5641 cccagaaatt cgaagcgaag ctgttgcaga tagcgataac cgataggagt atcagcaata
     5701 tcgactgatg caccatgccc gtcgccagcg tgatggatat aagagccact gtgattctcg
     5761 atataagcgc cgtgctgagc aaagctctct ttcttgtgcc ggttctgttc atgatgaaag
     5821 gtaccagcag ttggacgagc tgggaaagaa ccggcagaac tccaataacg gatataacaa
     5881 gctcgtctgc tctgtattcg agggctagac ctgtcagaat gaagccctgg gtgattatga
     5941 aaaagccgtt gtagaagaac gtatccatga aagatagtct tctggtcttc ttgtgcagtt
     6001 tctcagtgtc caattcattc atcgagccca tctaatcccc tttcgataac tattgtaaac
     6061 ccaaaacatt gaaagctgaa ttataagaaa agtgcggttg tcctttggac aaccgctatg
     6121 tttgagagat aaggggtgga attctccaca attgtattta tgcaaattcg tgccagtatt
     6181 ttggttaact ccggggtagt gtgtgaaatt gccccagaat tgactgcgta gttgtataat
     6241 cgctaagaac ggtttttaaa ccgctggagg tggtatcttg ctgtacagaa agatagccat
     6301 tggagagaac cttgagtctc taccctgtga agaacatatc cagagtctct tcgatgtaga
     6361 tttcgatttc ctggtgaacc tgggaaagga gactccgacg attcttttca tagatggtgg
     6421 aaactacagc aaagatatcc tcgatgactg gatctttgca ttcaccaaca taatacttat
     6481 aaacaacaac cccataaggg cgttcataat caggggttac tcagaagaga cgcaggaatt
     6541 caaaggatat tatcgagttg taggaagcta caaaagccct agacagatag ttcaagagat
     6601 aatagacagt atagacagga gccttgaaaa gataatcggc aacagcgaca gggtaattca
     6661 gatgaagcgg atgatcgtgc tttccatgtt ttacgatggt tctatcatga tactcggaga
     6721 gaccggctcg ggaaagaatc ttctggccga aactatcgcc agattgtctc cgcggggcaa
     6781 caaacccttc tactccataa actgcgccgc gatccctgaa acactgctcg agagcgagct
     6841 gttcggttac aaaaagggtg ccttcacggg tgccaccggc gaaaagatag gtctcataga
     6901 gcaagccgac aacggaacgc tctttctcga tgaaataggt gacatgcccc tgaatcttca
     6961 ggcaaagatt ttggccatca ccgaaaacag ggaatttttc aagattggag ccacaaaacc
     7021 gaccaaagtt gacgtgagat ttatcaccgc cacaaacaga accgaagact cggcgatgag
     7081 aaacgatcta aggtatagac tttcagcggt aaagatagat ctccctcctc tcagagagag
     7141 aaagatggat atcccgctga ttttcgacag cgttcttgag agaaagggat accttgtaag
     7201 gtttgaaaac atccctagag aattgaagga aaggttcctc tcgtacaact acccgggtaa
     7261 catccgcgaa ttgttgaaca tggtcgagga gtatcttgct ctgaacgatg ttgtacaggc
     7321 aactgtcgat tcatcgattt caaagaatat aaagatactc gctgaagaag ctctcgtagc
     7381 cagagtcgta aaagggacta cttacaaaga ctttcttcag gaaatctaca aaggtgtatc
     7441 cagagagata ctcatccaga gggccggagt tctcgataac gatataaatg cactggccgg
     7501 cgaatttgga ctcacaccga ggagaataag agatcttctg tcggagctgg aagctcaata
     7561 ggcctgaagc cccattcatc ggccttatgg ttgcgcggtt cgcgcaaaat ccctcgccgt
     7621 agataattaa tgtataatct caggagtagg aggtgttttt ttggctgtca gaaaactcaa
     7681 tcttccacag gagatagaag tagccgagct gttcggagaa tacgaccgga aggcgaagtt
     7741 cattcagaag aaactgaacg tgaagatatc gatcatcgga aacgagatct ggataaaggg
     7801 cgaggatgga aacggtatag aagttgcgaa gaacatagtt gatgagctgc tccagatgac
     7861 caaggacgga cattttgtgg aatggcgcga gttcgaatac atagtcgatc agcacctgca
     7921 acacgatgaa aaagatgagg aattcaaggg cgtttacagc gaagtaagag aaacatccct
     7981 gctttcaaat cgaataaggc ctaagactct cggccagaaa gaatacctgg aaaccatgaa
     8041 agaaaaggaa atggtcttct cgatcggtcc ggccggcaca ggaaagacct atctggcggt
     8101 tgcgatggcg gtggatttct tgaaagccgg aaaggttcag agaattattc tcaccagacc
     8161 ggccgtggag gctggtgaga agctgggata ccttccggga accctgctgg ataaagtgga
     8221 tccttacctg agacctctgt acgatgcgct catggagatg attcccaccg agaagctcgg
     8281 ttactacaga gaaaattcga tagttgaggt cgttccgctc gcctacatga gaggaagaac
     8341 gttgaacaac tccttcataa tcctcgatga agcgcagaac accacttatc agcaaatgaa
     8401 gatgttcttg accaggatcg gattcaactc cagagtcgtt gttacaggag acataacgca
     8461 gatagatctt ccacgcgaca ccaattcggg tctcgtggta atacagaaag ttcttggagg
     8521 tatagaatcg ataggttttt cctatctcac cgatcaggac gtggtaagga atcctctggt
     8581 taaggagata ataaaggcct acgacaccta tgaaagagac aagcaggcaa aaga
//
LOCUS       DAOOLY010000086         8517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9744_length_8517_cov_4.719333, whole genome shotgun sequence.
ACCESSION   DAOOLY010000086 DAOOLY010000000
VERSION     DAOOLY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9744_length_8517_cov_4.719333"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLD15_05860"
     CDS             <1..667
                     /locus_tag="PLD15_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63464.1"
                     /translation="LLNDHGVILSSVRLTYDDGGRLIRESHYGKDEKLESILLYKYEG
                     AVLRGIHSLDSSNRILEEVVTGGENGLNDRTQYSYSGDRAVRSSRTLADGYISVNEIV
                     DLTETDRPGRRVEISFALEPLVVMFDTEETLQTIISRNATGKITGVDFKSGNYLLAGF
                     KYYFDNAGRLLAREYAFGENSLRVDYRYDTAGNWIEERYFKLEDHEYRPVSTLARQID
                     YFE"
     gene            complement(785..1966)
                     /locus_tag="PLD15_05865"
     CDS             complement(785..1966)
                     /locus_tag="PLD15_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPB63465.1"
                     /translation="MVLSISLIIILSIPIISLFEKLRLPGLIGLIFLGVLLGPQILNL
                     IDRSLLDVSSEIRLLALIVILLRAGLGLDRNTMKKIGPLALRMSFLPGIFEGFSIMFF
                     AQWLGLDLVKGGMLGFIIAAVSPAVVVPEMLRFKESNMGKDREVPSTILAAASVDDVV
                     AITIFTVFLGIDRGENINIVTAVASVPLEIAFGIGTGALIGFLFALLFKKFHMRDTKK
                     VLLVLSSAFIFHQLENYMPVATLLGVMAIGFMLREKLPVAAERLAGKMERVWVLAEVF
                     LFVLVGASVSINAIGSSWQKGVLVVVLGLSFRSLGVAVSTLGSKLNLRERLFCMVSYI
                     PKATVQAAVGGIPLAMGVVYGDIILSVSILSIIITAPLGAIGIRYIGPRTLKKPAHFE
                     N"
     regulatory      complement(2013..2091)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2149..2352
                     /locus_tag="PLD15_05870"
     CDS             2149..2352
                     /locus_tag="PLD15_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63466.1"
                     /translation="MLTTFLAIVGALFLLYLAIRYFPAFIKLVIGVGVVLVVVFAFAG
                     LVVVFLPILIIIAIVLLVVKLVK"
     gene            2349..3260
                     /locus_tag="PLD15_05875"
     CDS             2349..3260
                     /locus_tag="PLD15_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPB63467.1"
                     /translation="MKTVDLTAIQVRFDATTFSRESFANAMGRYIRLARGLWRGERHV
                     TVFPEFFPTFLYPAFRGMALEGGPGKILLKYALKNTGLSLNPFRQAFLKHTLEIECFY
                     RETFGALAGEFKTYLLAPSILMPEVDIESSRGTFLRDKRLYNMAYLFNPQGRLIAKIA
                     KHNLTDAESGILFSRGSSFGNTAETSIGTLGVAICYDMFFESEVRQLDSRGCEILLVP
                     SCNFAPWKAPLTESTQERVWFRDGPISASKDRENIRFLVNAMAVGDVGGQTAQGRSSI
                     WHDGKVLAVSREFELEDVVNATVELDL"
     gene            3344..4273
                     /locus_tag="PLD15_05880"
     CDS             3344..4273
                     /locus_tag="PLD15_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPB63468.1"
                     /translation="MKIAVVHDEKPGEVGMKMLKSIAGVLEKHHEVFSVPLTPNFVEK
                     IESAGIDFAFNIATGGEGHRRQFYVPAILDFRNIPYTGSGVSANCLCIDKSITKVVMT
                     DSGIPTPKYVCFKQSHPVEEIDFYPAIVKPSCGGSAVGIWADSVVSDRIELERAVKRI
                     HREYGEPALVEEFIDGREFSIGIVGRTVLPILEIDFSFLPAGMERFYSYRVKQYLGEK
                     TGYICPADIPEDLKTKIESLSLELFDRLDLGDYARIDLRIDGQGEPYFLEVNSLPLLV
                     PDYSDIVKMAVAAGWEYDDLILAILDSAIERRR"
     gene            4278..5054
                     /locus_tag="PLD15_05885"
     CDS             4278..5054
                     /locus_tag="PLD15_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63469.1"
                     /translation="MKGGNVAFPPENEFTGLQSDYFMNHRERYELALKISRDLSGEMG
                     SNLISFCLYGSTCRGTDTRWSDLEMFAITIEETPQKSLLIGTVPAIVYTMTDSKLREI
                     LEKPGPQWPLYTGLMKGLMVLSGDTQAPSRYYSLALNVPGERLKEALKENLPGLVFES
                     YGRIFSSIVRKKHEDIYCAVVEVLLEMSTALCLLNGTHVNSSYFEGIRESFEFEKRPA
                     RYAALATRLWKSRNPFEIARDARELMKNYIALLESEGLIE"
     gene            5372..6850
                     /locus_tag="PLD15_05890"
     CDS             5372..6850
                     /locus_tag="PLD15_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HPB63470.1"
                     /translation="MYGRAVEFPDSGTWLNVDETLSMEKLKGHVVLLDFWTYCCINCI
                     HVIADLKWLEARFSNKPLVVIGVHSAKFENERQERNIQAAIQRYGIAHPVFVDNEYHL
                     WDRYAIRAWPSFVLIGSDGKILSRTSGEGKRDYLSNEIARALEDGERRGILSREKLEV
                     KPPVPTGGSTLSYPGKISFGNGETLFISDSNNNRILITRLGGKFDATVTDIIGGGSPG
                     MSDGGFEKATFNKPQGIVYSGGHLYIADTENHAIRRASIEERTVTTVSGDGLQGYNWN
                     YSGEASRARLNSPWDLQSDGAYLYIAMAGTHQIWRLDTRSNTIEAFAGNSMENIIDGS
                     LREASFAQPSGLFLNGRRLYVADSEVSAIRFIDLDGGTVHTLAGNGLFSFGHVDGSLS
                     HALFQHPLGIHGEGDFLYVADTYNHAIRRVSPEARRAETLLGTAGGGTCRVDGSECSF
                     LGLFEPNDVKYREGLLYIADTNNHLIRVFDGTKLHDLSIAYV"
     gene            6910..7392
                     /locus_tag="PLD15_05895"
     CDS             6910..7392
                     /locus_tag="PLD15_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63471.1"
                     /translation="MRKFFSLFVMVLVIVSLSMAVEIDLGVSIEVKQVLEQTLACARL
                     SILANVGVFEFDIPFSQVWPSERKFEFVDPLQTYAGFNFKLPITILYVKAGLGVQAAG
                     VIDLMAGKIQGWDLPSKARLALGVSESGLYLEGGISLGFTPALDNLFFCPYASVGVVF
                     "
     gene            complement(7331..8269)
                     /locus_tag="PLD15_05900"
     CDS             complement(7331..8269)
                     /locus_tag="PLD15_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB63472.1"
                     /translation="MRALGIDIGGTMIKTAIVDENGVISEKKLYPTQRKLREQLVQIV
                     REELAASNPEAIGIGTAGRVDPLTGEVNLATDNLTGWTGVPLKSFIQEETAVVTAVLN
                     DANAAALGEWFSNHRRTETLVMLTVGTGLGGGVVINGFPLLGKRGEAAEFGHVVIHPG
                     GRRCNCGKAGCAEQYVSMRLIHRLVAEVAGRELDRATLVEQYLGGDPIVGKAVDTVAG
                     DLAIVIDSIFLSFDPETVVVGGGICELGDRFLLSLREKLVAPSQSSLYAPEDVVLSLS
                     RNDSGIIGAAIFAMKNARTQLRPTRRGRKRDYPAPG"
BASE COUNT         2152 a         2127 c         2222 g         2016 t
ORIGIN      
        1 actgctcaac gaccacggag ttatcctctc ctcggtcaga cttacctacg acgatggcgg
       61 aagattgata agagagtctc attacggaaa agatgaaaag ctcgaatcga tcttgctcta
      121 caagtacgaa ggggctgttc tgaggggcat ccattctctc gacagcagca acaggatact
      181 cgaagaagtt gtcaccggcg gtgagaacgg acttaacgac agaacgcagt acagttactc
      241 aggagacagg gccgttagaa gttcgagaac cctcgcggac ggatatatct cggtaaacga
      301 gatcgtagat ctcaccgaaa ccgaccggcc gggtagaaga gtcgaaatct ccttcgcgct
      361 cgaacccctg gtcgttatgt tcgatacaga agagacgctt cagacgatca tatccagaaa
      421 cgcgactgga aagatcacgg gcgtcgattt caaaagcggc aactacctgc tggccggatt
      481 caaatactat ttcgacaacg ccggaaggtt actggccaga gaatacgcgt tcggtgaaaa
      541 ctctctcagg gttgattaca gatacgacac cgcgggaaac tggatagagg agaggtactt
      601 caagctggaa gatcatgaat acaggccggt ttcgacactc gcccgccaga tcgactactt
      661 cgaatgatac aaatgattag catttaataa tgacgacggg acttgtgggt gccaacggag
      721 ccttcgataa acatctataa tatggggtgc ccatcgaatc tggaaaatcg tttcaaggca
      781 cggatcagtt ttcaaaatgt gcaggttttt tcagcgttct gggaccgatg taacggattc
      841 caattgcacc cagtggagcc gttattatta tcgagagaat agaaacagag agaattatat
      901 ctccatatac cactcccatg gcgaggggaa tgccaccgac ggcagcctga acggttgcct
      961 ttggaatgta agataccata cagaatagcc tttcacgaag gttcaacttc gaccccaggg
     1021 tcgaaactgc aactccaagg cttctgaatg atagccccag gactacgacc aacacacctt
     1081 tttgccatga actgccaatg gcatttatac ttacgcttgc acccacaaga acgaagagga
     1141 aaacctctgc cagtacccac acacgctcca tctttcccgc cagtctttcg gcggcaaccg
     1201 gtagtttctc tcttagcatg aacccgatcg ccattacacc tagcaacgta gccaccggca
     1261 tatagttttc cagttgatgg aaaataaaag ccgatgatag gaccagtaga actttcttag
     1321 tgtccctcat atggaatttt ttgaataaaa gagcgaaaag aaaaccgatg agtgctccag
     1381 ttccgattcc aaaggctatt tccagcggta cgcttgcaac agcagtaact atgttgatat
     1441 tctctcctct atctatacca aggaaaacag tgaatatggt tatggcaact acatcatcaa
     1501 ctgaggccgc agcaagtatg gtcgatggta cttccctgtc ctttcccatg ttgctttcct
     1561 tgaagcgaag catttctgga actacgactg caggagaaac ggcggctata atgaaaccaa
     1621 gcattccacc cttcaccaaa tccagaccga gccattgagc aaagaacatt atcgagaaac
     1681 cttcgaaaat acctggaaga aagctcattc tcaaggcgag gggaccgatc ttcttcatag
     1741 tattacggtc aagaccaaga ccggcacgaa gaagaattac tatcaaagct agtaacctga
     1801 tttcactcga aacgtccaac agtgacctgt ctatgagatt gagaatctga ggaccaagta
     1861 gaacaccgag aaatataagt cctatcaggc cgggaagccg cagtttttcg aaaagagaga
     1921 tgatcggaat tgaaagaata attatcagcg agatactcaa taccattttt acctccagaa
     1981 cacggcaacc aacgacagcc accacaaatt tgacagtagt agaagctatc agcgtgcaac
     2041 gcggttctgg atggttaaaa ctccatggca agcttcattg ccttaggtat tatatgatgc
     2101 gataagttat actgtcaaat agatgctgaa cggaggtgag agagtgagat gctgaccact
     2161 tttcttgcga ttgtcggtgc gttgtttctg ctttatctgg cgataaggta cttccccgcc
     2221 ttcatcaagc tggtgatagg ggtcggagtc gttctggtgg tggtttttgc cttcgccggt
     2281 cttgtagtgg tcttcctgcc gatcctgatt attatcgcta tagtcctgct ggttgtgaag
     2341 ctcgttaaat gaagaccgtc gatctaacgg ccatccaggt ccgtttcgac gcaacgacgt
     2401 tttccaggga atctttcgcg aacgcgatgg gccgatacat tcggctggcc cgtggtctct
     2461 ggaggggcga gcgtcatgtc acggtctttc cggagttctt tccgaccttc ctctacccgg
     2521 ccttcagagg catggccctg gaaggtggtc cgggaaagat ccttttgaag tacgcgctca
     2581 agaatacggg tctctctctc aatccattca gacaggcctt tttgaaacat acgctcgaga
     2641 tagagtgttt ttatcgcgaa acgttcggag cgctggccgg ggaatttaaa acttatctgc
     2701 tggcgccctc gatactcatg cccgaggttg atatcgaatc ttcgaggggg accttcttga
     2761 gggataagag gctgtacaac atggcttatc tcttcaatcc tcagggccga ctcatcgcga
     2821 agatcgccaa acacaatctt acggacgcag agagcgggat actcttctcc aggggaagct
     2881 ccttcggcaa cacggccgag acttcaatcg gaacactggg cgttgccatc tgttacgata
     2941 tgtttttcga gagcgaagta cggcaactcg attctagggg ctgcgagatt ctacttgttc
     3001 cttcctgcaa cttcgccccg tggaaggccc ctttgaccga atcgacgcag gagagggtat
     3061 ggttcagaga tggtccgatc agcgcgtcga aagacaggga gaacatacgt tttctggtga
     3121 acgcgatggc cgtcggcgat gttggtgggc agaccgccca gggcagatct tccatatggc
     3181 acgacggcaa ggttctggcc gtctcgcggg agttcgagct ggaagatgtc gtgaatgcca
     3241 cagtggaact ggatttgtag ccgggcggac tttagagaat cgcgggtagt ataatctggt
     3301 cggcagttgt ataaaccgga ccgaattcaa aggaggtata tgagtgaaaa tagcggtggt
     3361 acacgacgaa aaacccggtg aggtcggcat gaagatgctg aaaagtatcg ccggtgtttt
     3421 agagaaacac cacgaagtat tctccgtgcc gctcacgccg aatttcgtcg aaaagatcga
     3481 gagcgcaggt atagatttcg ccttcaacat cgcgaccgga ggcgaaggcc accggagaca
     3541 gttctatgtg cctgccatac tcgacttccg gaatatacct tataccggtt ctggtgtatc
     3601 ggccaactgc ctctgcatag ataagtcgat caccaaagtt gttatgaccg acagcggcat
     3661 accaaccccg aagtacgttt gcttcaaaca gtctcatccg gttgaagaga tagatttcta
     3721 tccggctata gtaaagcctt catgcggagg aagtgccgtt ggaatatggg ccgattcggt
     3781 ggtcagtgat aggatcgaac ttgaaagggc agtgaagaga attcatcggg agtacggaga
     3841 accagctctg gttgaagagt tcatagacgg tcgagaattc agtatcggga tagtcgggcg
     3901 cacggtgctc cccattctgg agatagactt ctctttccta ccggcgggga tggaacgttt
     3961 ctactcctac agggtcaagc agtatctagg cgagaaaacc ggttacatct gcccggcgga
     4021 cataccggaa gatctgaaaa caaagatcga aagcttatcg ctcgagctct ttgataggct
     4081 tgatctgggc gattatgcgc gtatagacct aaggatcgat gggcagggcg agccgtattt
     4141 cctggaggtc aactctctcc cgctgcttgt gcccgattac tcggatatcg taaagatggc
     4201 ggtggcggca ggctgggaat acgacgatct gatactcgca atactggatt ccgcgataga
     4261 gagaagaaga taatcgggtg aaaggaggca atgtcgcttt tcctcccgag aatgaattca
     4321 cgggtctcca gagcgactac tttatgaatc atcgagagag gtacgaactg gctttgaaaa
     4381 tctcccggga cttatccgga gagatgggta gcaatttgat ttctttctgt ctttacggtt
     4441 ccacttgccg gggtacggac acgcgctggt ccgacctgga gatgttcgcc ataaccatag
     4501 aagagacacc tcagaaatct cttctgatcg gtactgtccc cgcgatcgtt tacacgatga
     4561 ccgattcgaa acttcgcgag attcttgaaa agcccggtcc acaatggcca ctgtacaccg
     4621 gtcttatgaa gggactgatg gttctttccg gagacacgca ggcgccgtcc aggtattatt
     4681 cgctagcgct taacgtgcca ggcgagagac taaaagaggc tcttaaagag aatctgcccg
     4741 gattggtctt cgaatcatac gggaggatct tctccagcat agtcagaaaa aaacatgagg
     4801 atatctactg cgcggtagtc gaggttctgc tagagatgag caccgcgcta tgtctgctga
     4861 acggcacgca cgtcaacagc agctacttcg aagggataag ggaaagcttc gaattcgaaa
     4921 aaaggcccgc gcgttacgca gccctcgcca ccaggctatg gaagagccgc aaccccttcg
     4981 aaatagctcg cgacgccaga gaacttatga aaaactatat cgcgcttctg gaaagcgaag
     5041 gccttatcga gtagtgagtc tgcatccaca agattattat gaaatgatga gaaggttgag
     5101 agtatcaaga ataagacgtt gaagcctggt gatttcagtc agaatatggc catacctttc
     5161 cgaataatcg attctagtca aagtctccat ctaaagaagc agttccctca ccgaatactt
     5221 ctcattcaac ccggtgccct tcagcctctt tcgcaatacg cttatcagaa taaccgctat
     5281 taactgaagg aagaagtctc ctatccattc gggaatacat ctttgatcga tttttgtatc
     5341 attccagtca taagtgaaag gagtgatgaa tttgtacgga agagcggtag aatttcccga
     5401 ttcgggaacc tggttgaatg tcgatgagac tctttcaatg gaaaaactca agggtcacgt
     5461 ggttcttctc gatttctgga cgtactgttg catcaattgc attcacgtta ttgccgatct
     5521 gaaatggctt gaggccaggt tcagcaataa gccgctggtg gttatcgggg tgcattcggc
     5581 gaaattcgaa aacgagcgac aggaaaggaa catacaggcg gcgatacagc gatacggaat
     5641 agctcacccc gtattcgtgg acaacgaata ccacctctgg gaccggtacg ctataagggc
     5701 atggccctct ttcgttctga tagggtccga cgggaagatc ctatccagaa cctcaggcga
     5761 gggaaaacgc gattaccttt caaacgagat cgccagggcg ctggaggatg gagagagaag
     5821 aggaatattg agccgggaaa aattggaagt caagcccccc gttcccaccg gcggatcgac
     5881 gctttcctac ccggggaaga tctctttcgg aaacggggaa acgctattca taagcgattc
     5941 aaacaacaac agaattctca taaccagatt gggcggcaag ttcgacgcga ccgttacaga
     6001 tattatcggc ggcggctctc cgggcatgtc cgacggcggc ttcgaaaagg ccacgttcaa
     6061 caaacctcag ggcatcgtct attccggcgg ccatctatac attgccgaca cggaaaacca
     6121 tgcgatccgc agggcctcca ttgaagagcg gacggtgacc accgtttccg gcgacggcct
     6181 tcaggggtac aactggaatt acagcggaga ggcttcgagg gcgaggctga actcgccgtg
     6241 ggatcttcaa tcagacggcg cctatctcta tatcgcgatg gccgggaccc atcagatctg
     6301 gagattggac acgagaagca ataccataga ggccttcgcc ggaaacagta tggaaaacat
     6361 aatagacggc tctctgagag aggcgagctt tgcccagccg agcggactgt tcctcaacgg
     6421 ccgcaggctt tacgtagccg attcggaagt ttcggccata aggttcatag atctcgacgg
     6481 gggaacggtc catactctgg ccgggaacgg tctcttttcc ttcgggcacg tggacggtag
     6541 cctgagccac gctctctttc agcatccctt aggaattcac ggcgagggcg attttctgta
     6601 cgtcgccgac acttacaatc acgccataag gagagtttct ccggaggcca ggcgcgcaga
     6661 aacgcttcta ggaacggccg gaggcggtac ttgcagggtc gacggttcag aatgttcctt
     6721 cctggggctt tttgaaccga acgacgtgaa gtatcgtgaa ggacttcttt acatcgccga
     6781 taccaacaat cacctgatca gggtcttcga cggtacgaag cttcacgatc tgagtatcgc
     6841 gtatgtttaa tattgcggat tattgatgta tcataataat ggcttcattt taattcgggg
     6901 gtgttgctca tgcgaaaatt cttctcactt tttgtgatgg ttctggtaat cgtttcgctt
     6961 tcgatggcgg tagaaataga tctcggcgtg agtatcgagg tgaagcaggt gcttgaacag
     7021 acgctggcct gcgccaggtt gtctatactg gcaaacgtgg gggttttcga gttcgatatt
     7081 cccttctccc aggtttggcc ttcggagagg aaattcgaat tcgtcgatcc tcttcagaca
     7141 tacgccggat tcaatttcaa gcttcccata acgatcctct acgtgaaggc cggtctcggg
     7201 gtgcaggcgg ccggcgtaat cgaccttatg gccggaaaga tacagggctg ggatctccct
     7261 tccaaggcac gtctggctct gggcgtatcg gaatcgggac tttatctcga agggggtatc
     7321 tctctgggat tcaccccggc gctggataat ctctttttct gcccctacgc gtcggtcgga
     7381 gttgtgttct agcgttcttc atcgcgaaga tcgccgcgcc tatgattccg gagtcgtttc
     7441 gcgaaagcga cagcaccacg tcctcggggg cgtaaagaga cgattgggag ggagcgacca
     7501 gcttctccct gagagatagc aggaagcgat cgccgagttc gcatattccg ccccccacaa
     7561 cgacggtctc aggatcgaaa ctcaggaaga tcgagtctat gacgatcgcc agatcaccgg
     7621 cgaccgtatc gacggccttc ccgactatcg ggtcgccgcc caggtactgt tcgaccagcg
     7681 tcgccctgtc cagctcgcga ccggcaacct cggccaccag cctgtgaatg agtctcatgg
     7741 agacgtactg ctcggcacac ccggcctttc cgcagttgca gcgcctcccg ccgggatgga
     7801 taaccacgtg tccgaattcg gccgcttcac cgcgctttcc caggagcgga aagccgttta
     7861 ttaccactcc tccgccaaga ccggtcccca cagtcagcat gaccagcgtt tccgttcggc
     7921 gatggttgga gaaccattcc cccagagcgg cagcgttggc atcgttcaaa acggccgtaa
     7981 cgacagcggt ctcctcctgt atgaaactct tcaggggcac tcccgtccag ccggtcaggt
     8041 tgtccgtggc caggttcacc tcgcccgtga gaggatcgac tctgccggcc gtgccgatgc
     8101 cgatcgcttc gggattgctg gcggcgagtt cttccctcac tatctgcacg agctgttctc
     8161 tgagctttct ctgcgttgga tagagctttt tttcggaaat cactccgttt tcatcgacaa
     8221 tcgctgtctt gatcatcgtt ccaccgatat ctataccgag agcacgcatt cacacacctc
     8281 tcttgagaac tcttctgaag tcgttccacg cctgtccgga gaagtaataa agccccgtct
     8341 gcaagatcat acatcatttt tcttatggat ccgccgtctg tggcataata tatagggatt
     8401 ctttttcaag gagaaggaaa tgactgaagc tttcggcttt tctttaccca cagcggttct
     8461 gttgttgaac ataggtatag tctcattcat aggcataata tgcctcatag gctattt
//
LOCUS       DAOOLY010000087         8472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9810_length_8472_cov_4.923488, whole genome shotgun sequence.
ACCESSION   DAOOLY010000087 DAOOLY010000000
VERSION     DAOOLY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9810_length_8472_cov_4.923488"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLD15_05905"
     CDS             <1..697
                     /locus_tag="PLD15_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63473.1"
                     /translation="LFLGPLPYFLTSVPAFVLFTVLRRKVPYISLVIAPLLPIIVELI
                     ALMMANFRGGFLVGDIVCLVTALLGLGEERPKERMIRRLGLSGKGSRNMVLSLFYTFG
                     RVERIEKVNMSQSYAMVHEGSFHFSVNIPGSGRVNMEIPLCDIEEVAVHQSTSSGDLY
                     YPSLRDMFLPARNIKLVGKPRIQDYFLVVRVAEDAYTFYEEPKTVLELQEEIEREREL
                     SVRSKQSPGEPPA"
     gene            786..3389
                     /locus_tag="PLD15_05910"
     CDS             786..3389
                     /locus_tag="PLD15_05910"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPB63474.1"
                     /translation="MKELGTRYSPSDLEKKWYSCWKENGHFEPKGSGKPFTIMIPPPN
                     ITAALHMGHALNLVLQDIMTRFKRMQGFRALWVPGEDHAGIATQNAVEKNLAKKGIDR
                     KKLGREKFLETTWEWTREYRQKIREQIEAMGCSVDWSRERFTLDERLSQAVRKSFVQL
                     YKEGLIYRGKYIVNWCPRCSTVLSDEEVEHEDEKGAFYHIKYPIKGSDEFVVIATTRP
                     ETMLADTGVAVYPSDERYLSLQGKTAILPLVNRELPVVADRYVDPAFGTGALKVTPAH
                     DPNDFQIGMRHNLALVEVFDKDAKITEAGGKYAGLDRYEARKEVVKDLEEQGYLIKIE
                     PMVHAVGRCYRCDTVVEPSLSDQWYVKVGPLAERGIKAVEDGEVVFFPETWRKVYLNW
                     MNEIRDWCISRQLWWGHRIPVWYCDECGEIIVEEHDPESCPGCGSGKIRQDEDVLDTW
                     FSSQLWPFTTLGWPEETEDLATYYPTSVLITGFDIIFFWVARMIMAGYHFRGEKPFEH
                     VFINRLIRDKTGQKMSKSKGNGIDPLDVIKEYGTDAMRFTLAILAAQNHDIKLDISFF
                     DIYKKFANKIWNAARFAIMNLQDFESLDLEKAELALEDRWILSRLQRSLESMTVSLES
                     YDFPAAAKTMYAFFWNEFCDWYIESAKPRLYEGKGDRKVAQNVLVRVLDISLRMLHPI
                     MPYITEELWQKLPGSDGALIVADWPKVEEKMIDPEAENAYSRLMDTVRGIRNVKAEMN
                     IPPSTRTDVYYLGTPLEKSYLDLIAHLSNSKAITLKDCKSRGSVSAWGDQSNLFYVVL
                     GEIDVPSEINRLQTKLEKERKDLEKTEVKLSNEDFLKNAPEEVVLENRERLVTSRDNI
                     ERIEKIIEDLR"
     gene            3386..4711
                     /locus_tag="PLD15_05915"
     CDS             3386..4711
                     /locus_tag="PLD15_05915"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPB63475.1"
                     /translation="MSRTYSQTIDYLYNSRPYGQIKYGLYRIKELLERLGNPQKACPV
                     VHITGTNGKGSVAATIATVFTLHGLKTGLNVSPHIVSFRERIQLDGHFIKEDEVVNLL
                     EEMQPALEEMDARGEEYSPSFFEVVTAMSFMYYREKKADVAVVEVGLGGRFDASNVID
                     SALVSIITSVGLDHMNILGDSEEKIAFEKSGIIKSGCPVVTGVTRPSVLGVIEETARN
                     LNSPFYAYWRDFNATGSSYSTNDNSFDYYGDRTLRDLHINLNGEHQTMNAAISLKALE
                     LSFEGLCLEMKEETLRKALRRIFWPGRFEVMHVQGKEIILDGAHNPEAAKVLKKTVER
                     YYPGQRLTLLFGSLDDKDFESNVRALAPVADRVVVCRVPNHRSVHPESVAGIWKKYTS
                     NVEYIDSYREALDTLLKGGEKRFLICGSLYLVSELRNILTGVKEYAGRR"
     gene            4695..5291
                     /gene="ruvA"
                     /locus_tag="PLD15_05920"
     CDS             4695..5291
                     /gene="ruvA"
                     /locus_tag="PLD15_05920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPB63476.1"
                     /translation="MLEGVEGRVVEKRETEVVVQVGGFFLRLNCTPDTLNRLAKGETT
                     FLLTHMAYSQDRPPELYGFADREELDLFNTLLKMSKIGPKSAMKILSATAPSRLRNLI
                     ATRNTGELSRLPGVGKKTAERMVVELASVLNIVEGEEEMKSLPMTQRSTGEDAVIALA
                     SLGFDEQQARKTVTGLLKKNPEIDTQELIKTALKEIRG"
     gene            complement(5281..8268)
                     /locus_tag="PLD15_05925"
     CDS             complement(5281..8268)
                     /locus_tag="PLD15_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPB63477.1"
                     /translation="MIENDVVLFIDTVPDEDIISKTIGRVPVVLYGSAVKALRLIGVE
                     ERGAIEESRVSLSDSPWDNRGFQSYRDHPLFNGLHGGFYSIYLNACEEMPDVFYYRGK
                     RAKVVAVEKRYISYIRERALIWLHTVNGFPVLSIGGFLPLECEDNPYRSEVERFVENT
                     LGFMSEGGQGGSYWAEAHCVCRHVFFDRKLEIPSLSPVENWPNVSIPVERAGGYLSTS
                     GERLLANMSVGRIEEVWAHPFRILKYLEFSIDGKPLSLSAGSISCSPERVEFKLEFGR
                     MNVFSSLKESFLFVQFDLDDRREHTIGVSFESDMRIMWPMDEAYSGDRFYGVFEKGVH
                     FSTQDGQMRASIVNSNPVRIEIGDDGRSVIGRFESRFEGTGCVAIIASINGEEFPRTI
                     DMQKEIELARDFYTAYLERGRVRTDDRRLNEALDLARVGAVKFRATTPGIGTGLMAGY
                     ASSRPGWFSARPGYAWYFGRDSLWCSLAFLDIGDFETVRENLELLVKYRRLDGKIYHE
                     LTSSGVVHYDAADSTPLFLYALYRYARHTGDLDFVRKNLDSLRAALDYCAGTDSDGDG
                     LIENTVAGHGWVEGGRLYGASASFYLNCIWLAALRGVGELAEKLHDGELRNAAATLEK
                     KATAGLEKFHDPSKGYALGIDQYGRPMPHRTVMASMGAIFEAVSGESIGEQIGEFATD
                     DFSTDWGVRIIGKSSGIFNPRGYHEGSVWPLFTGWAALAQFKIGADLDGYNHTLANLY
                     TCRDFSGGYIPEVLDGETYELSGVCPHQAWSETMGFHPFYEGVLGFEPDSLKGTLRLG
                     PSIPPNLERLEAGPLIFGDNSLDLRYASSRVFGGFIEMTGRFELKFKREVALYFTPHI
                     PAYAERIELRVNGRERPVKVYRGTTATACRIEPEGPVSGRELEIEVRYSVPFLLMPVE
                     PQLLSGKESCQPRIVSIARGTGGWELTLAVSGGRSCIPFLASKGLKAENAVLGDGKLY
                     VSSDRTGYNPAKVLLRHESPSTP"
BASE COUNT         2047 a         2375 c         2323 g         1727 t
ORIGIN      
        1 gctctttctc ggtccactcc cctatttcct gacgagtgta cccgccttcg ttctctttac
       61 cgtcctcaga cggaaagtgc cgtatatatc gctggtgatc gcccctctgt tgccgatcat
      121 cgtggaattg atcgccttga tgatggccaa cttcagggga ggttttctgg ttggcgatat
      181 agtctgcctg gtgaccgcgc tcctggggct gggagaggaa aggcctaaag agaggatgat
      241 caggcggctt ggtctctccg gtaaaggtag cagaaacatg gtcctatcgc tcttctacac
      301 cttcggcagg gtcgagagaa tagagaaagt caacatgagc cagagctacg ccatggtaca
      361 cgaggggagt ttccacttca gcgtgaacat acccggctcc ggtcgggtca acatggagat
      421 acccctgtgc gatatcgagg aagtggccgt ccaccagagc acatcgtcgg gcgatctgta
      481 ttatccctct ttgagggata tgtttctgcc cgccaggaac atcaagctgg tgggaaagcc
      541 caggatacag gactacttcc tggtggtgag ggtcgccgaa gatgcctaca ctttctacga
      601 agagcccaaa accgtgctgg aactccagga ggagatcgaa agagaaaggg aactctccgt
      661 ccgatcgaag caatcgcccg gtgaaccgcc ggcttaacct caaatgcaca acctctcccc
      721 attctctccg gcacttcgtg atataattcc tgttaatcgc tgcaagctcg caagggggta
      781 gtcctatgaa agaactggga accaggtaca gtccttccga cctggagaag aagtggtact
      841 cctgctggaa ggagaacggc cacttcgaac ctaagggtag cggaaagcct ttcaccataa
      901 tgataccgcc accgaacata acggcggctc ttcacatggg tcacgcgctc aacctcgtcc
      961 ttcaggatat catgacccgg ttcaagagaa tgcagggttt cagggctctc tgggtcccgg
     1021 gagaggatca cgccggcatc gccacgcaga acgccgtcga gaaaaacctc gccaagaagg
     1081 gtatcgacag gaagaaactc ggccgtgaaa agttcctgga aaccacctgg gagtggacca
     1141 gggaatacag acagaagatc agggagcaga tagaggccat gggctgttcc gtcgattgga
     1201 gcagggagcg attcaccctg gacgagcgat tgagccaggc cgtcagaaaa tccttcgttc
     1261 aactctacaa agaaggcctg atatacaggg gcaaatacat agtcaactgg tgtcccagat
     1321 gctcgacggt tctctcggac gaagaagtgg agcacgagga cgaaaaggga gccttctacc
     1381 atataaaata ccctatcaag ggcagcgacg agttcgtagt gatagccact accagaccgg
     1441 agaccatgct cgccgatacg ggagttgcgg tctatccctc cgacgagaga tacctgtcgc
     1501 tccagggaaa gacggccata ctgcctctgg taaacaggga gctgcccgta gtggccgaca
     1561 ggtacgtcga tccggccttc ggtacggggg cgctcaaggt gaccccggcg cacgatccga
     1621 acgacttcca gatagggatg aggcacaatc tggcgctcgt ggaggtcttc gacaaagacg
     1681 cgaagataac cgaagccggt ggcaagtacg ccggtctgga tagatacgag gcccggaaag
     1741 aggtggtcaa ggatctcgag gaacagggat acctgatcaa aatagaaccg atggtgcacg
     1801 cggtgggaag gtgctaccgt tgcgacacgg tggtcgaacc ctcgctctcg gaccagtggt
     1861 acgtcaaggt cggaccgctc gccgagaggg ggataaaggc cgtcgaagac ggagaggtcg
     1921 tcttcttccc cgaaacctgg aggaaggtct atctcaactg gatgaacgag ataagggact
     1981 ggtgcatctc cagacagctc tggtggggcc accgcatccc ggtctggtac tgcgacgagt
     2041 gcggagagat catagtcgag gagcacgatc ccgaaagctg ccccggctgc ggctctggca
     2101 agatccggca ggacgaggac gtactcgaca cctggttctc ctcacagctc tggcccttca
     2161 cgacgctggg ctggccggaa gagacggaag atctggcgac ttactacccg acgagcgtcc
     2221 tcataaccgg cttcgatatt attttcttct gggtggccag aatgatcatg gccggctacc
     2281 acttcagggg cgagaaaccc ttcgaacatg tcttcataaa ccgcctgata agagataaga
     2341 ccggccagaa gatgtcgaaa tcgaagggga acggcatcga cccgctggat gtgataaaag
     2401 agtacggaac ggatgcgatg cgcttcacgc tggccattct ggcggcacag aatcacgata
     2461 taaagctgga cataagcttt ttcgacatat acaagaagtt cgccaacaag atctggaacg
     2521 cggccagatt cgccataatg aacctgcagg acttcgaatc cctggatctt gaaaaggcgg
     2581 agctggctct cgaagaccgc tggatactct cgcgtctcca gagatctctg gaatcgatga
     2641 ccgtgagcct ggaatcctac gattttccgg ccgccgccaa gacgatgtac gccttcttct
     2701 ggaacgaatt ttgcgactgg tacatagaaa gcgccaaacc ccggctttac gaagggaagg
     2761 gcgacaggaa agtggcgcaa aacgtgctcg tcagggtgct cgatatatcc ctcaggatgc
     2821 ttcaccctat catgccgtat ataaccgagg agctctggca gaagctgccc gggtccgacg
     2881 gggctctcat agtcgccgac tggccgaaag tagaagagaa gatgatcgac ccggaggccg
     2941 agaacgctta ttctcgcctc atggatacgg tgaggggtat cagaaacgtc aaggccgaga
     3001 tgaacatccc gccttcgacc agaaccgacg tgtactacct gggaactccg ctggaaaaaa
     3061 gctacctcga cctgatagcc catctgtcca attccaaagc gataaccctc aaagactgta
     3121 aatccagggg cagtgtctcg gcctggggag accagtctaa cctcttctac gttgtcctcg
     3181 gagagataga cgtaccctcg gagataaacc gtctccagac aaaactcgag aaagaacgta
     3241 aagaccttga aaaaaccgaa gtgaaactgt ctaacgaaga cttccttaaa aacgctcccg
     3301 aagaggttgt cctggagaac agagaacgtc tcgtaaccag cagggacaac atcgaaagga
     3361 tagagaagat aatagaagac ctgagatgag cagaacatac agccagacca tagattacct
     3421 ttataactcg cgaccttacg gacagatcaa atacgggctc tacagaataa aagagctcct
     3481 cgaaaggctg ggcaacccgc aaaaggcctg cccggtcgtc catataaccg gaaccaacgg
     3541 caagggtagt gtagccgcaa ccattgccac agtcttcacg ttgcacggcc tcaagacagg
     3601 cctgaacgtc tctccgcaca tagtgtcctt cagagagagg atacagctcg acggccattt
     3661 tataaaagaa gatgaggtcg taaacctcct ggaagagatg caaccggctc tggaggagat
     3721 ggatgccagg ggcgaggagt actcccccag tttcttcgaa gtggtgacgg cgatgagctt
     3781 catgtactac agggaaaaga aagccgatgt cgccgtcgtg gaagtcgggc tgggcgggag
     3841 gttcgacgca agcaacgtta tcgactcggc cctggtctcg ataatcacca gcgtaggcct
     3901 cgaccatatg aacatactcg gcgacagcga agagaagatc gccttcgaga aatccggcat
     3961 aatcaaatcc ggctgtccgg ttgtaaccgg tgtcacccgt ccgtcggtgc taggggtgat
     4021 cgaagagacg gcccgcaatt tgaactctcc gttttacgcg tactggcgcg atttcaacgc
     4081 gaccggcagt agctattcga ccaacgacaa cagcttcgat tattacggcg acaggacgct
     4141 gagggatctg catatcaatc tcaacggcga gcaccagacg atgaacgccg ctatttcgct
     4201 caaggcgcta gagctctcgt tcgagggact gtgtcttgaa atgaaggaag agactctgcg
     4261 aaaggcgctc aggcggattt tctggcctgg aaggttcgaa gtgatgcacg tccagggcaa
     4321 ggagataata ctcgacggcg cccacaaccc cgaagcggca aaagttctca aaaagaccgt
     4381 ggagagatat taccccggcc agaggctgac gctcctcttc ggaagtctcg acgacaagga
     4441 ctttgaaagc aacgtcaggg cgctcgcgcc ggtggccgac agggtcgtgg tctgcagagt
     4501 tcccaaccac cgcagcgttc atcccgagag cgtcgccggc atctggaaaa aatacacaag
     4561 caacgtcgag tacatagatt cttaccggga ggcgctcgac accctcctca aaggcgggga
     4621 gaaacggttt ctcatatgcg gttcgctcta tctggtgagc gagctcagga acatcctcac
     4681 cggagtgaag gaatatgctg gaaggcgttg aggggagagt agtcgaaaag cgtgagaccg
     4741 aggtcgttgt gcaggtcgga ggcttttttt tgaggttgaa ctgcactccc gacaccctga
     4801 acagactcgc gaagggcgag acgacttttc tcctcaccca catggcctat tcgcaggaca
     4861 gacctcccga gctttacggt ttcgccgata gggaggagct ggatctcttc aacacattgc
     4921 tcaagatgag caagatcggt ccgaaatcgg cgatgaagat cctatcggca acggccccct
     4981 ccagattgcg aaaccttatc gccaccagaa ataccggcga gctgtccaga ctgccgggcg
     5041 tggggaaaaa gacggccgaa aggatggtcg tcgaactggc ctccgtgctg aacatcgtcg
     5101 aaggcgaaga ggagatgaaa tcgctgccga tgactcaacg ttcgaccggc gaggacgccg
     5161 tgatagctct ggcctcgcta ggcttcgacg agcagcaggc gagaaagacc gtcacaggtc
     5221 ttctcaagaa aaatcccgag atcgatacac aggaactgat caaaacggcc ttaaaggaga
     5281 tcagggggta gagggcgatt cgtgccgaag caagacttta gctgggttgt accccgtccg
     5341 gtcgctggaa acatacagct tcccgtcccc gagaacggcg ttttcggctt tcaaaccttt
     5401 cgaggcaagg aagggaatgc acgatcttcc gcccgaaacg gcgagcgtta actcccagcc
     5461 tccagtgcct ctcgcgatcg agacgatcct gggctggcag ctctcctttc cactgagcaa
     5521 ctgcggttct acgggcatca ggaggaaggg gacgctgtat ctgacctcga tttccagttc
     5581 ccttccgctc accggtcctt ccggctcgat cctgcaggcc gtcgccgtcg taccccggta
     5641 aaccttcacc ggcctctcgc ggccgttcac tctcaattcg atcctttcgg cgtacgccgg
     5701 tatatgggga gtgaagtaaa gggcaacttc cctcttgaat ttcaattcga agcgcccggt
     5761 catctctatg aagccgccga agactctgga agaagcgtaa cggagatcga gcgagttgtc
     5821 gccgaagatc agcggcccgg cttcgagcct ttcaagattc ggcggtatgg aaggcccgag
     5881 cctcaacgtt ccctttagag aatccggttc gaagcccagc acaccttcgt aaaagggatg
     5941 aaagcccata gtttccgacc aggcctggtg cggacagact cccgacagct cgtaggtctc
     6001 gccgtcgagg acctccggta tataaccgcc cgagaagtcc ctgcaggtgt acaggttggc
     6061 cagagtgtgg ttgtaaccgt ccagatcggc tccgattttg aactgcgcca gagcggccca
     6121 gcctgtgaag agaggccaga cgcttccctc gtggtaaccc cttgggttga aaatgcccga
     6181 gctcttcccg attatcctga cgccccagtc ggtggaaaag tcgtcggtgg cgaactcccc
     6241 gatctgttcc cctatactct ctcccgacac agcctcgaag atcgccccca tcgaagccat
     6301 gaccgttctg tggggcatcg gccttccgta ttggtctatg ccgagggcgt atcccttcga
     6361 aggatcgtgg aatttttcca gtcctgcggt ggccttcttc tccagagtag cagccgcatt
     6421 tcgtaactcc ccgtcgtgga gtttttcggc caattccccg actcccctga gcgccgccag
     6481 ccagatacag ttgagataga acgaggcgct cgccccgtaa agcctgcccc cttcgaccca
     6541 gccgtgcccg gcaaccgtgt tctctatgag gccatccccg tcagaatccg ttccggcgca
     6601 gtaatcgagc gccgcacgca gagagtcgag atttttcctt acgaaatcga ggtcgcccgt
     6661 gtgcctggcg tacctgtaga gggcgtacag aaagaggggc gtcgagtcgg cggcatcgta
     6721 gtgaaccacg ccgctcgaag tcaattcgtg atagatcttc ccgtcaagcc tccggtactt
     6781 gaccagaagc tcgaggtttt ccctcaccgt ttcgaagtcc ccgatatcga gaaaggccag
     6841 cgaacaccac agactgtcgc ggccgaagta ccaggcgtac ccgggcctgg cgctgaacca
     6901 ccccggcctg ctagaagcgt acccggccat aaggccggtt ccgattccgg gagtggtggc
     6961 cctgaacttt accgcgccca cacgcgccag atctagagcc tcgtttagac ggcgatcgtc
     7021 cgttctcact cttcccctct ccagataggc cgtgtagaag tctctggcca gctctatctc
     7081 tttctgcata tcgatcgtcc gggggaactc ctcgccgttt atactggcga ttatcgcaac
     7141 gcaaccggtc ccttcgaagc ggctctcgaa cctcccgatc accgacctcc cgtcgtcgcc
     7201 gatctctatt ctcaccggat tcgagttgac gatcgaagct ctcatttgac cgtcttgtgt
     7261 ggagaagtgg acaccctttt cgaagacgcc gtagaacctg tcgccactgt aggcttcgtc
     7321 catgggccac atgattctca tgtccgattc gaacgaaaca ccgatagtgt gttcccgtcg
     7381 atcatccaga tcgaactgca cgaagaggaa ggactccttc aggctgctga agacgttcat
     7441 tcttccgaac tcaagtttga actccaccct ttcgggcgaa caggaaatcg accctgcact
     7501 cagagagaga ggttttccgt cgatggagaa ctccagatac ttcagaatcc tgaaggggtg
     7561 ggcccagacc tcttctatcc gcccgacgct catattggcc agcaatctct ccccggacgt
     7621 gctcaggtaa ccaccggccc tctcgacggg gatggagacg ttcggccagt tttcgacggg
     7681 cgagagcgag ggaatctcca attttcggtc gaagaaaacg tgcctgcaaa cgcagtgtgc
     7741 ctccgcccag taagatcccc cctggccgcc ttcagacatg aatccaagag tgttttcgac
     7801 gaacctttcg acttcgctcc gatacgggtt gtcttcgcac tcgagcggta aaaagccgcc
     7861 gatcgaaaga acgggaaaac cgttgaccgt gtggagccag atcaaggccc tctcccttat
     7921 atagcttata taccgctttt ccaccgcgac cactttcgcc ctctttcccc tgtaataaaa
     7981 aacgtcgggc atctcttcgc acgcgttcag atagatgctg taaaagccgc cgtgcagccc
     8041 gttaaagagc gggtgatctc tgtacgactg gaagccccta ttatcccagg gagaatcact
     8101 cagagagact ctcgactcct ctatcgctcc tctctcttcg acaccaatca gccggagagc
     8161 cttcacggca gagccgtaaa gcactaccgg cactctgcca atagttttag atattatatc
     8221 ttcatcgggg actgtatcta taaatagaac tacatcattt tcaatcactg tatttaaatc
     8281 acgactgacg ctgaattttg tttttgaaac gatttcttca aggcggcttt tccgcctttc
     8341 aagtattcta atcatatcgg cccgttacct acctcggcca gagttcttgg agggtgtatt
     8401 cgacggcgaa gttccggtgg ggctcgttct ggtaccagta agcgacggtc gcgatatcgt
     8461 ctgtcaaagg ct
//
LOCUS       DAOOLY010000088         8466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9823_length_8466_cov_5.054928, whole genome shotgun sequence.
ACCESSION   DAOOLY010000088 DAOOLY010000000
VERSION     DAOOLY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9823_length_8466_cov_5.054928"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PLD15_05930"
     CDS             complement(<1..1020)
                     /locus_tag="PLD15_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63478.1"
                     /translation="MKFPGLLLNDLLLMRKSRAFVLLLFLLPLILALISTITSESGGY
                     TDLKIAIVNEDKTFIGLFFLQYATSMLKGENIVQLSSRDQIDGVLEDLDGVFIIPRGF
                     ANKLLFQEPSELIFVPNPGSMQTAVAIYQVLNNVLKEFKALPVVADPEFMKGVEIDPE
                     YVAPAITVEGIRERKLNFSSLLFPMVLSITIMFVTAIGAASGTFEDRRNGVIDLLKLS
                     NLSGMSYMAAKTLSFSIFSTLQVLVFLLAGKVLGLDVASNIFLYLLVIEVYIILFTSI
                     GLSIGMVFSSSRSAQLTSVTLVTSLLILSGTIIPHSMFPDWLGIFSKNLPTTRLMVSL
                     QGVSML"
     gene            1157..2491
                     /locus_tag="PLD15_05935"
     CDS             1157..2491
                     /locus_tag="PLD15_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63479.1"
                     /translation="MKKICFLVVLTIAAVSLASVTSMAERLPSDSHTVLVMEDLKENY
                     AKSKEVVLMDMILNQLAIEQLVAQYVEMLAYNNGLDPKDIYKAFDGDLGLAVWRDSSG
                     DDRLIAVLGPMKDPRTLKSAAEKILPQLLPVDFKIKMATDSEYLYVGDIDQYSQAVRG
                     FDSTKLIDGLPAGFGYLYSEMDESLTKASFRNQDKLIVMETLTLPTTEESIEAFRKAF
                     SGGEVTDLEDMSHLPLVSIFARLGDISMLADYAGDQLTSVPLDIEGLVPGNLNTDELD
                     DYLTGDFLVDIDLSVDDIFASLMGSQGTSSSQTSQMNITYIARVGFSGSLDVLKNSIE
                     KSSPETKIEVKGDTLLVEGQHMWIDNGWLFISSRDRDKTVQTLDNSVPLLNSAAYAEL
                     SSMIHEPGFARFFVDSGYVLTGLMGMDINSGLLFGTSYLAQSGAVKGILLIK"
     gene            2602..2826
                     /locus_tag="PLD15_05940"
     CDS             2602..2826
                     /locus_tag="PLD15_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPB63480.1"
                     /translation="MRLDKFLKQSRIIKRRILAKEAIEKGFISRNGIQAKPSSEVKPG
                     DTISIRYSNRTLVVKVLEDFEIEILKEIKE"
     gene            complement(2816..3541)
                     /locus_tag="PLD15_05945"
     CDS             complement(2816..3541)
                     /locus_tag="PLD15_05945"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPB63481.1"
                     /translation="MIGEKIISFSEIDSTNKFAIENLDRLPYGTVIWSLSQTFAYGRY
                     GRSWSSGDGGLWFSIIFKPLQITEPNAYTKLLSVAVVETVRDLKYRNAQIKWPNDILI
                     DGKKVAGILTESVFAGERLKGIVVGVGLNVNNELPKELLSSATSLSYLKNEYIPLNLL
                     IEKILRRVEVLRKKYMIKGKTKYLTRRWKKLQAFVEGDEITVSLNENEKLTGIIKRIS
                     PVSLQLQSYDGEIHNVVSGEIIL"
     gene            complement(3612..4412)
                     /gene="panB"
                     /locus_tag="PLD15_05950"
     CDS             complement(3612..4412)
                     /gene="panB"
                     /locus_tag="PLD15_05950"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPB63482.1"
                     /translation="MNVREIIASKDSRKLSMVTAFSYFEAKFALESEIDMILVGDSLG
                     TYVMGKKDTLSVTMEEMLWSLKAVRRGARDGFIIADMPFGSYQQSREKAVENAIAFLR
                     HGANALKIEGGYETAELIKYLVDRGFPVMGHVGITPHHASLEGTYQVQGKDEKSIKRL
                     IESVKNLEEAGVFAILLELVVEEVAGKLTQEASVPVLGLGSGRFCDGQFLKWHDLLGI
                     NDESEPRYVKRYANLKENIVSALKHFDRDVKEGRFPVEDHAFGGGEEI"
     gene            4632..5708
                     /gene="rodA"
                     /locus_tag="PLD15_05955"
     CDS             4632..5708
                     /gene="rodA"
                     /locus_tag="PLD15_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPB63483.1"
                     /translation="MKARFEWQLPFVMAILMAFGLVVLYSATFGEREEYMFGRQVVWA
                     LLSWAVFVFTAYVLKRKVWRDFAIALMALSIAGLVLVLFLNPIEGSRRWIDLGIASLQ
                     PSEIAKFSLILFLGYLYSLETRRYLSFLAGLLATFLIMGLIYLEPDLGTTIIIVVIWY
                     FMTLISKKFDRLMIVMTALIAVAVPAVLMFGLESYQRDRILSFLNPEEYSSGAAYNTI
                     QAIRAIGSGGWTGKGFVQGTMNRLGYVPADHTDFIFSVIGEEWGFVGAITVILLYSLL
                     LWRIWAITRRTGDFFGRLVMSGFFAMFAFHVVENIGMNLGLLPVTGIPLPLISYGGSS
                     SMIFALQLGIVQSYASRRADYDKK"
     gene            5741..6655
                     /gene="ftcD"
                     /locus_tag="PLD15_05960"
     CDS             5741..6655
                     /gene="ftcD"
                     /locus_tag="PLD15_05960"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPB63484.1"
                     /translation="MRLIESVPNFSEGRRAEVIEEIVNEGRKIDGVWILDYSSDPNHN
                     RSVVTLTGVPEAIEEALFDMTKKARDLIDLKNHSGEHPRMGSADVIPLIPVMNTTKDE
                     CIELSRRLGRRIGEELSIPVYLYEDSATSPERVSLSNIRKGEFENFNSKITQEAWKPD
                     FGPSSVHPSAGVVAVGCREYLIAFNVNLGTDNIEIANKIAKAIRHISGGFRYVKALGF
                     RLEDRGIVQVSMNMTNYRKTPLFRVYEVIKSEAERYGVSIVGSEIVGLTPLQALVDVA
                     EHYLKLEKFDSSSILEKRVLDFISREGE"
     gene            6657..7868
                     /gene="hutI"
                     /locus_tag="PLD15_05965"
     CDS             6657..7868
                     /gene="hutI"
                     /locus_tag="PLD15_05965"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPB63485.1"
                     /translation="MKLDLAILNLNSIVTPVAHGPVRGRDMARIECIKESNIGILDGK
                     IEYVGPEIPVAEKYIDGRGLIAMPGFIDCHTHIPFTGNRSKEFLMRLEGRSYMDIMKA
                     GGGIRSTTEAVRKAPATSLMAESSRFLDSMLSLGVTTVEGKSGYGLDRDGELKQLRVL
                     EALDEIHPVDVFSTFLGAHALPREYSSKDEYLSSLIETFDEVHKLTDTIDIFCEDGVF
                     NVDQSRRYMLAARDRGFRIRIHADELSSSGGGLLAVELGAVSADHLISADDATIKALA
                     KSDTIATLLPGTSFFLNESFASGRELIDRGAIVAIASDFNPGSCNIYNPFIVMFLAVT
                     RCGLKVEEAITAYTANAAAVLGAEDRKGLIREGYDADLVLLRAGDYSEVVYNFSRDIV
                     SNVIKSGTVIG"
     gene            7865..8332
                     /locus_tag="PLD15_05970"
     CDS             7865..8332
                     /locus_tag="PLD15_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPB63486.1"
                     /translation="MNIRVILTGKPKSDFVVQGIEQYDKWLKPYVRIEIRHLPLGGGT
                     DREREKIKEKEGKNYLKLLRPDETVVLLHEQGEQYSSVEFAQKLQKWQNIGVKNLSFL
                     LGGPLGFSDELLSREWQKLSLSRMTFTHEMALMLLLEQLYRAYSILDNRTYHY"
BASE COUNT         2193 a         2050 c         2123 g         2100 t
ORIGIN      
        1 cagcatcgag accccctgga gagagaccat caaacgggtt gtagggagat ttttgctgaa
       61 gatccccaac cagtcgggaa acatcgagtg aggaattatg gtaccgctca gtatcaacaa
      121 cgaggtgacc agagtaacag acgtcaactg ggcgctcctc gaagagctga agaccatccc
      181 gatcgagagg ccgatcgagg tgaagagaat tatataaact tcgatgacca gcagataaag
      241 gaagatgttc gaagccacat cgagacccag aacctttccg gccagaagga acaccagaac
      301 ctgcagagta gaaaagatgg agaaactgag cgtcttggcg gccatataac tcatcccgga
      361 gagattagag agtttcaaca ggtcgatcac tccgttccgc ctatcctcga aggttccgct
      421 tgccgctcca atggcggtca caaacattat ggttatcgaa agaaccatgg ggaatagcag
      481 agaagaaaag ttcaattttc tctcgcgaat tccttcgacg gtgatcgccg gagcaacgta
      541 ctccgggtct atctccacac ccttcatgaa ctccggatcg gccaccacgg gaagagcttt
      601 gaactctttc aacacgttgt tgagcacctg gtagatcgcg acggccgttt gcatcgatcc
      661 cggattaggt acgaagatca attcggacgg ttcctggaac aggagtttgt tcgcgaatcc
      721 ccttggaatg atgaagaccc cgtctaggtc ttccagcact ccgtctatct ggtctctact
      781 agagagctgc actatgttct cgcccttgag cattgaagtg gcgtattgca ggaaaaagag
      841 acctatgaag gttttgtctt cgttgacgat cgcgattttc aggtccgtat agccaccgct
      901 ttccgatgtt atggtagaaa tgagggccag tatcagaggc agcagaaaca acagcagaac
      961 gaaagctcga gactttctca tcagaagtag atcgttcagc aaaagccctg ggaatttcaa
     1021 ttgattagcc tccacgccaa gcgaattatt tctcgcgata ccggtcagaa ccgtacgatg
     1081 atagaattat atcatcgaga accagctcgg gagttcaagt gtcatagaag acaacagcac
     1141 taaggaggtg gacggtttga aaaagatatg ctttttagtg gttttgacga tagccgctgt
     1201 ttctctggcc agcgtaacct cgatggccga aaggcttccc tcagattccc ataccgtact
     1261 cgttatggag gatttgaaag aaaactacgc caaatctaag gaagtcgttt tgatggatat
     1321 gatacttaac cagctggcca ttgaacagct ggtggcccag tacgtagaaa tgctggccta
     1381 caacaacgga ctggatccta aggacatcta caaggccttc gatggcgatc ttgggcttgc
     1441 ggtttggaga gattcctcgg gcgacgaccg attgatagct gttcttggcc ctatgaagga
     1501 tccgaggact ctgaaaagcg cagccgaaaa gatactgccg caacttcttc cggttgattt
     1561 caagataaag atggctaccg acagcgaata cctgtacgtc ggggacatcg atcagtactc
     1621 tcaggctgtc agaggcttcg attcgacgaa actcatagac ggccttccgg caggattcgg
     1681 ttatctctat tcggagatgg acgaaagcct cacaaaggct tccttcagaa accaggacaa
     1741 gcttatcgtg atggaaactt tgacccttcc gacgaccgaa gaaagcatag aggccttcag
     1801 gaaagccttc tccggtggag aggtcacaga cctggaagac atgagtcacc ttccgctggt
     1861 ttccatattc gccaggctag gcgatataag catgctggca gactacgccg gagatcagct
     1921 aacctccgtt cctctggata tcgaaggcct ggtcccggga aatctcaaca ccgacgagct
     1981 agacgactac cttaccggtg acttcctggt cgatatagat ctctccgttg acgatatctt
     2041 cgccagtttg atgggctctc agggaacttc ctcatcccag acatcccaga tgaatatcac
     2101 ctatatagcg agagtggggt ttagcggttc actggatgtc ctcaaaaatt ccatcgaaaa
     2161 atcgagtccc gaaacaaaaa ttgaagtcaa aggagatact ctactcgtgg aaggtcagca
     2221 catgtggata gataacggat ggttgttcat ctcctccaga gatagagata agacggttca
     2281 gacgctcgac aacagtgtcc cgttgttgaa ttcagcagcc tatgccgagc tctcttcgat
     2341 gattcacgaa ccaggtttcg ccaggttctt cgtggatagc ggatatgtcc taaccggatt
     2401 gatgggaatg gatatcaaca gtggtctgct tttcggcacg agttatcttg cacagagtgg
     2461 cgcagtgaaa ggtatacttt tgatcaaata aaaaaagagc cccttcgcca agcggagtag
     2521 cgcggggcag aacatctcgt tgacattatt ataccctttc gacatagtgt tagtcaagtg
     2581 tatactcagg ggtggtcggg gttgagactg gataaattcc tgaaacagag cagaatcata
     2641 aagagaagaa tactcgccaa agaagcgatc gaaaagggct tcatatcgag aaacgggatt
     2701 caggccaaac cgtcttcaga agtaaagccg ggagacacaa tctcgataag atattccaat
     2761 cgaacgctgg tggttaaagt tctggaagac tttgaaatag agatattgaa agagatcaaa
     2821 gaataatttc acccgaaact acgttgtgga tctccccgtc gtaagattgc agctgaagtg
     2881 acaccgggga gatcctcttt attatacctg tcaatttctc gttttcgttg agagatacgg
     2941 ttatttcatc cccctcgacg aaggcctgaa gcttcttcca ccttctcgtg agatacttgg
     3001 tctttccctt tatcatatac ttctttctca agacctcaac cctgcggagt attttctcta
     3061 tcagcaggtt cagaggaata tattcgtttt tcaagtaaga gagcgaagtt gcagaactca
     3121 agagctcctt aggcagctcg ttgttcacgt tcaaaccgac ccccacgacg attcctttca
     3181 gtctttctcc ggcgaaaacc gattcggtca gtattcccgc aaccttcttc ccatcgatga
     3241 gaatatcgtt aggccatttt atctgagcgt ttctgtactt cagatcccta acggtctcaa
     3301 caaccgcaac ggatagaagt ttcgtgtatg cgttcggttc tgttatttga agcggtttaa
     3361 aaattatcga aaaccagagt ccgccgtcac cagaagacca cgagcgaccg tatcttccgt
     3421 aggcgaaagt ctgcgataga gaccagataa ccgtgccgta aggcagcctg tcgagattct
     3481 ctatggcgaa tttattggtc gaatctattt cagagaaaga gattatcttc tccccgatca
     3541 tcaaatcgct tcatccctct ccaagtttgt tatttggccc gccagttcaa gacggatcat
     3601 tatcaccttt tctagatctc ttctcctcca ccgaaggcat gatcctctac cgggaacctc
     3661 ccttctttaa cgtcccggtc gaagtgtttc agggcgctca cgatgttctc cttcaaattg
     3721 gcgtaccttt taacgtacct cggctcagat tcatcgttta tgcccagaag atcgtgccac
     3781 ttcaagaact gtccatcgca gaaccggccc gatccaagtc ccagcacggg aacggaggct
     3841 tcctgagtga gttttccggc gacttcttcc accaccagct ccagaagtat cgcgaagacc
     3901 ccggcctctt caaggttttt taccgattcg atgaggcgct tgatgctctt ctcgtccttt
     3961 ccctgtacct gatacgtccc ctcgaggctg gcgtgatgag gagtgatccc tacgtggccc
     4021 atgacgggaa atcctctatc gacaaggtat ttgatcagct cggccgtttc ataacctccc
     4081 tcgatcttca atgcgtttgc accgtgccgt aaaaaagcga tggcgttttc tacggccttc
     4141 tctctcgatt gctggtacga accgaagggc atgtcggcga tgatgaaccc atctctcgcc
     4201 ccgcgccgga cggccttcag agaccagagc atctcttcca tggttaccga cagagtatct
     4261 ttcttaccca tcacgtatgt accgagcgaa tcgcccacca gaatcatgtc gatctccgac
     4321 tctaaagcga acttcgcctc gaagtagctg aaagccgtta ccatcgataa ttttcttgaa
     4381 tctttcgacg cgattatctc acgtacgttc attcgagaag caccttccct tcagagaact
     4441 cgcttttatt gtgagataac aaccagtaaa ggaccgttgt attccctcat ctacgattat
     4501 acatttttct ctctactggt acagtaacgg cattctcact gtcaatattt cccggcgatt
     4561 gcaggctgtt ttgcttgtgt aatctcagtt tctctcacca ggttatataa ttgatggaag
     4621 gagagagttt gatgaaagct aggttcgagt ggcaacttcc ctttgttatg gcaattctca
     4681 tggcctttgg tctggtggta ctttacagcg cgacctttgg cgagagagag gaatacatgt
     4741 tcgggcgcca ggtggtgtgg gctcttcttt cgtgggccgt cttcgttttc acggcgtacg
     4801 tactgaaaag aaaagtatgg agagatttcg ccatcgcgct catggcgctg tccatagcgg
     4861 gccttgttct ggtgcttttc ttgaatccca tcgaaggatc gagaagatgg atagatcttg
     4921 gcatcgccag tttacagcca tcggaaattg cgaagttttc cttgatactc ttcctcggtt
     4981 atctttattc gctcgagacc aggcggtatc tttctttttt agcaggcttg ctggcgacgt
     5041 ttctgatcat ggggttgatc tatctggaac ccgatcttgg aacgacgata atcatcgtgg
     5101 tgatctggta ctttatgaca ctgataagca aaaaattcga cagattgatg atagtcatga
     5161 ccgctttgat agccgtggca gtgccggccg ttctcatgtt cgggctcgaa tcctaccaga
     5221 gagacaggat tttgagtttt ctcaatcccg aagaatactc ttccggggcc gcctacaaca
     5281 ccattcaggc tataagggcg atcggttcgg gtggatggac cggaaaggga ttcgtgcagg
     5341 gtaccatgaa cagactgggt tatgtgccgg ccgatcacac cgatttcatt ttttccgtga
     5401 taggcgagga gtggggcttt gtaggtgcga tcacggtcat attgctttat tcgctactgc
     5461 tatggagaat ctgggcgatc accagaagaa ccggtgattt ttttggtaga ctcgtaatga
     5521 gcggtttttt cgccatgttc gcttttcacg tggtggagaa tatcggtatg aatctcggtc
     5581 ttctcccggt aaccggtatt ccccttcctt tgataagtta cggaggatct tcatcgatga
     5641 tattcgccct gcaacttgga atagtgcaga gttatgctag cagaagagca gattacgata
     5701 agaagtagaa aaaacagcga tatttccagg gaggtatttt atgagactta ttgaatcagt
     5761 acctaatttc agcgaaggca ggagagccga ggttatcgaa gagatagtga acgaaggtag
     5821 aaagatcgat ggggtatgga tactggatta ctcgagcgac cccaaccaca atcgctcggt
     5881 tgtaacgctc acaggggttc ccgaggcgat agaggaagcc ctcttcgata tgacgaagaa
     5941 ggcccgagat ctgatagatc tgaagaacca ttccggagaa catcccagaa tgggctccgc
     6001 cgatgtcatt ccgctgattc cagttatgaa cacaacaaaa gacgagtgta tagaactttc
     6061 cagaaggttg ggcaggagaa tcggcgagga actgtcgata ccggtttacc tttacgagga
     6121 ttccgcaacc agcccggaac gtgtcagtct ttcgaacata aggaagggtg agttcgagaa
     6181 cttcaactcc aagatcaccc aggaggcctg gaaacccgat tttggacctt cttctgttca
     6241 tccttcggct ggcgtggtcg ctgtcggttg cagggaatac cttatcgcct tcaacgtcaa
     6301 tctgggaacc gacaacatag agatagccaa caagatagcc aaggccataa gacacataag
     6361 cggcggcttt cgctatgtca aagccctcgg gttcagactg gaagacagag gcatagtgca
     6421 ggtctccatg aatatgacca actacagaaa gacaccgctc ttcagggttt acgaagtcat
     6481 aaagtccgag gccgaacgat acggagtttc tatcgtggga tcggaaatag ttggccttac
     6541 acccctgcag gccctcgtag atgttgccga acactatctc aaactggaga agttcgattc
     6601 gtcgtcgatt ctcgagaaga gagttctcga cttcatttcg agggaaggag agtagagtga
     6661 aactcgatct ggccatactg aaccttaact caatcgtaac tccggtcgcc cacggaccgg
     6721 tcagggggag agatatggcc cgcatcgaat gtataaagga aagcaacatc ggaatccttg
     6781 atgggaaaat cgaatacgtc ggaccggaaa taccagtcgc tgaaaaatat atcgacggcc
     6841 gtgggttgat cgccatgccg gggtttatag actgtcacac acacatcccc ttcacaggta
     6901 acaggagcaa ggagttcctc atgcggctgg aaggccggag ttatatggac ataatgaagg
     6961 ccggcggtgg aataagatcg acaacggaag cggtgagaaa ggccccggct acctctttga
     7021 tggccgagtc ttcgcgattt ctcgactcca tgctctccct gggagtcacg actgtcgagg
     7081 gtaagagcgg gtacggtctc gacagagatg gggagctcaa gcagcttcgt gttctcgaag
     7141 cgctcgacga aatacacccg gtcgatgtct tctcgacttt tctgggtgct catgccctgc
     7201 cgcgagagta ttcatccaaa gacgaatacc tctcctcatt gatcgaaacg ttcgatgaag
     7261 ttcacaaact gacagatact atcgatattt tctgtgagga tggggttttc aacgtagatc
     7321 aatcgagacg ttacatgctc gccgccagag accgtggctt caggatcaga atacacgccg
     7381 acgagctctc ctcatcggga ggcggtttgc ttgccgtgga actgggggcc gtttccgccg
     7441 accacctgat atcggccgac gatgccacca tcaaggcgct tgcgaaaagc gacaccatcg
     7501 ccacgctcct tcccggaacc tccttctttt tgaacgagag tttcgcaagt ggcagagaat
     7561 tgatcgatcg gggggcgatc gtcgccatag catcggattt caacccgggc tcctgcaaca
     7621 tatacaaccc cttcatcgtc atgtttctcg ccgtcaccag gtgcggcctc aaggttgaag
     7681 aagcaataac cgcctacacc gccaacgcgg cggccgttct cggggccgag gacaggaagg
     7741 gattgatcag agaaggttac gacgccgatc tagtgctact gagagccggg gattactcgg
     7801 aagtggtgta caatttctcg agggatatag tctcgaatgt cataaaaagc ggaactgtaa
     7861 tcggatgaac ataagggtaa ttctaacggg aaaaccgaaa tcggattttg tcgtccaagg
     7921 tatcgagcaa tatgacaaat ggctcaaacc gtacgtgaga atcgaaatcc ggcatcttcc
     7981 cctgggcgga ggaacggaca gagagaggga gaagatcaag gagaaagaag gcaaaaacta
     8041 cctgaaactt ttgagacccg atgaaactgt cgttctgttg catgaacagg gtgaacaata
     8101 ctcatcggta gagtttgcac agaagctaca aaaatggcag aatataggag tgaagaatct
     8161 gtcttttctc ctgggaggac cgctcggttt cagcgacgag cttctctcga gagagtggca
     8221 gaaattatcg ctgtccagaa tgactttcac acacgagatg gctctcatgc ttctactcga
     8281 gcagctctac agggcctatt ctattctgga caacaggaca taccattact gatctggagg
     8341 tgtaaaaaag aagtgggcga ccctactagt agcgaggacc ctgtgtcgct tttattgaac
     8401 ctggcctttc tgattctatt gctgtatcta tcgggacttt tctctggatc tgaagcggct
     8461 ctaatg
//
LOCUS       DAOOLY010000089         8381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9957_length_8381_cov_4.546361, whole genome shotgun sequence.
ACCESSION   DAOOLY010000089 DAOOLY010000000
VERSION     DAOOLY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9957_length_8381_cov_4.546361"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLD15_05975"
     CDS             complement(<1..883)
                     /locus_tag="PLD15_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HPB63487.1"
                     /translation="MKIAVIGAGNGGQALAAVLAMRGNEVALYNRSQNRIQPILKSKK
                     LKVEGESGGTARLKYVGTDMGKAVSGAELIMIVVPAFAHAEVARKLSQFISEGQVVIL
                     NPGRTGGALEFDKIFKECGVRNKAVLAEAQTFLFASRISGPGMVRIFRIKNAVPVAVL
                     PSKDNELLMPVIGDVIPEFTLTDNVLYTSFNNIGAIFHPGAILMNAGWIESTHGDFQF
                     YLDGISPAVARVLEEVDRERCEVTSKLGVQAMGAREWLDYAYDARGEDLYNAIHNNEG
                     YRGIMAPISMENRYIIED"
     gene            complement(1088..2515)
                     /locus_tag="PLD15_05980"
     CDS             complement(1088..2515)
                     /locus_tag="PLD15_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPB63488.1"
                     /translation="MSAIVIFTGDNEELLGESLDLMAHRGEEKTIYESVDGFQMASTA
                     GRAFSASKMRHSSPEGAAVIDGFLLSPPGSDWSEEVMRLYRELGEGFLERLEGSFSLA
                     VNDGKETIIARDPIGLKPLYYGRIGRETIFASELKAIAPYCDEVALFPPGHYFTREEG
                     FKEYSTCREIIDSGETVTDKEKAIEDVREALEDSIRKRLEVLDCEPSIFLSGGLDSSC
                     IAASLKQFVGNFKTFTVGKSDGEDPGCARVVADHLGTEHLEYFYDLDEMLKVLPDVIY
                     HLESFDPLLVRSSIPNFILSKLASENGCEFVFMGEGADELFAGYDYMKKIESGEEIEA
                     ELLRITENGHRSGFQRDDRMALAHGIEYDVPFMDPDMLKLAFSIPVDWKLHGKDKTEK
                     WILRKAFEDRLPDEIVWRKKKKFSIGAGSYDLMERYAQESVSQEQFERDREKAGIRTK
                     EESLYFNIFKEVYHCEGAIETVYRN"
     gene            2871..4640
                     /locus_tag="PLD15_05985"
     CDS             2871..4640
                     /locus_tag="PLD15_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPB63489.1"
                     /translation="MRSILINGEVTYNATSQILNSNTFKKILSELIKDSEEKKNSLLP
                     LFEPFLKNTDTVDTAQKYDIEGIIELLLALTIDPIEKIDGSPYYSFPKMSNKKDLLVR
                     FVESLFSLWRNKHRFIIKRDEYTPNRFDRIFKQMLLVRTNTDLKGLVLSMYRQILINV
                     SDIRLKILRQEPGGAQVGFIVDKPAVDPRMKLKNADWLYELEYVWSIVFEPPVIFYTK
                     SNKRRGVFKVNDRPVLDRIDITDPKDWFGFPIHVGTKLIYVVVNKEYLALASGLGNLF
                     ELASFDIIKNKKPDGIYILGIDNQFFEEPDDFNGVIYKENDGTYVGLVGDDPSIDYFG
                     YMKKMILTIHNLLIIDEGRLPIHGALAHIKLRNGKKANVMLIGDSGAGKSETLDALNR
                     LQGEVSEVNILIDDMGSLDITGDGTVVAYGTETGAFVRLDDLQPGYAYSTMDRSIFMN
                     PNEINARVIVPYSNYTEIIKPTRIDYFLYANNYNEDVKEKIAFFKKAEDANDVFSKGA
                     RMAKGTTTEKGLTYSYFANPFGAVQRREKHEAIAQRYLKSMIDGGVKVGEVMTRLGIH
                     GFEMEGPLQAAKALLEAIEELES"
     gene            4707..5624
                     /locus_tag="PLD15_05990"
     CDS             4707..5624
                     /locus_tag="PLD15_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63490.1"
                     /translation="MPKTVIDVRNLKKHYGETKAVDDISFQLMEGEVLAILGPNGAGK
                     TTTVEMLEGLRKPDSGAVEYFEEKLPPSSDRVKEEIGVLLQSSSFFEYLSVKETLDLF
                     RGLYTKGVSSDELIEEVSLDEKKKTYTKNLSGGQLQRLAVAVSLVNDPKVVFLDEPTT
                     GLDPQARRMLWETVLNLKKRQKTIILTTHYMEEAEHLADRIIIMDHGKIISRGTLDEL
                     IASIETEDIVSFRVKGEEEIPQEFLQMEGLKHVENRTYAVTTKDVERTLGRLFERSKK
                     FEILLDDVTIRKPNLEDVFITLTGRKLRD"
     gene            5637..6800
                     /locus_tag="PLD15_05995"
     CDS             5637..6800
                     /locus_tag="PLD15_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63491.1"
                     /translation="MSRMLKFTSGLFKNEIREFQVMFWSFIFPLILYFILTSVFGGMA
                     SGNSQGISFNLGIVKEENLTGFGRVLDEVLGSISSHGGPFVQREYGNLDEALEDLKNG
                     KQDIVLVIPKGTNAKMTGSLMLQVGDVPLRMHYISGKESSSIAANILGEIMNEVNLEI
                     KKRQEENYIDIASTNNVVSAKQEKGFDYNEYIFPGVALMMILSVSLFNSPIGLIQYRV
                     SGTNKKLYTTPLKPLEYFSAHFVKLVFTMLMSLVLLYIIALTVYRVQGTIFDPRFILS
                     LLYSMLVSVSFGLMVASFAKKLSTATVVGQSLYQVMMFMGGLYFPVFDLPWGLRWIVY
                     LLPTTYLVELSRRFMGYTIAPISMTWLIVVPLIWMIFSVSVFALNFRKVMGYE"
     gene            6793..7857
                     /locus_tag="PLD15_06000"
     CDS             6793..7857
                     /locus_tag="PLD15_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63492.1"
                     /translation="MSKSKLKPMFIAFAKEAFRNKLEVFFTLFFPMLFLILFGFFFSG
                     SGGYSKSKLGLFQSGTMDIKPIIEESGAWSVTIYETRDKLLEAVSGGEVLLGVTFDGT
                     GIEYSYREGDLSQRSTITMAQSSISAAVEKRINDAKTVIVLEKIPETAGRTTATDADY
                     TMSGVIAISILSAGMFSVISVFGRYRKRGVLKRFKITPVKPMTFILGSTLTRFLVSFV
                     SILLIILVSKLLFKTTFQIDWPIFLISVISSTLGMMALGLFLTLIFKNPETADSAASI
                     LMVIMIFLAGIYFPLAFLPDYLRVVSMFMPVKYVGELMRHSLGIELLPVMNFVLINLV
                     LSLCGIALLYFTGRRYLKAS"
     gene            7977..>8381
                     /locus_tag="PLD15_06005"
     CDS             7977..>8381
                     /locus_tag="PLD15_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPB63493.1"
                     /translation="MSLGIKITDGIHWIGVNDFETHLFEALWPLPKGVSYNAYMIVDE
                     KVVLIDTVKGPYFSTYLDKVKSLLPAGKTVDYLVVNHMEPDHSGSIKVLREAFPEVKI
                     IGNQKTADMLGAFYGLTDNLVVVRDGEELSLGE"
BASE COUNT         2147 a         2089 c         2074 g         2071 t
ORIGIN      
        1 cgtcttcgat tatgtagcgg ttttccatgc tgatcggcgc catgatgccc ctgtaacctt
       61 cgttgttgtg gatcgcgttg tacagatctt ctcctctggc atcgtaagcg taatcaagcc
      121 attctctggc gcccatagcc tgaacgccga gtttgctggt aacctcgcaa cgctctctat
      181 ctacctcttc aaggaccctc gcgacggccg ggctgattcc gtcgagatag aactggaagt
      241 ctccgtgagt ggattctatc catccggcgt tcataagtat cgctccggga tggaatatcg
      301 caccgatgtt gttgaagctc gtataaagta cgttgtccgt aagcgtgaat tcgggaatca
      361 catccccgat caccggcatg aggagttcgt tgtcctttga aggcagtaca gccaccggta
      421 cggcgttctt gatccggaat atccttacca tccccggacc ggagatcctg gaggcaaaga
      481 gaaaggtctg tgcctcggcc agtaccgctt tgttcctcac gccgcactct ttgaatatct
      541 tgtcgaactc aagggcacct ccggttcttc cgggattgag aatgacgacc tgaccttccg
      601 agatgaactg tgagagcttt ctggctactt ccgcgtgcgc gaaggcgggc acgactatca
      661 tgatgagctc agcgccggaa acggcctttc ccatatccgt cccgacatat ttcaaacgcg
      721 cggttccacc cgattctccc tccactttga gttttttgct tttcagtata ggctggattc
      781 tgttctgact tcgattgtag agcgcaactt cgttcccacg catggcaagc accgctgcaa
      841 gagcctgtcc gccattcccc gcacctatta ccgctatttt cattcgacac ctcctgttct
      901 tcagacaacc actgaaaaga cctgcgttta actgcaattc tcaagctacg atcgatacac
      961 attacaatta tactttattc gtcgcacgtc cttttttaaa gtcgtgtata accattcaga
     1021 ggttttattg gtgttcggcg atacggtgtg aatcttcgaa agattccgac gacctgccga
     1081 ttttctatca gttgcggtaa acggtctcta tggctccttc acaatggtag acttctttga
     1141 aaatattgaa gtaaagcgac tcttctttgg tccttatgcc ggccttttct ctatctcttt
     1201 cgaactgttc ctgactaacc gactcttgcg cgtatctttc cataaggtcg taagaaccag
     1261 ctccgatact gaacttcttc tttttcctcc acactatctc gtccggcagc cggtcttcga
     1321 aggcctttct gagtatccac ttctcggttt tgtctttacc gtgcagtttc cagtcaaccg
     1381 gtatggagaa ggccagtttc agcatatccg gatccatgaa gggaacatcg tattcgatac
     1441 cgtgagccag agccattctg tcgtctctct ggaagcctga acggtgaccg ttctcggtta
     1501 ttcgcaacag ctcggcctcg atctcttctc cggactcgat cttcttcata taatcgtacc
     1561 cggcgaagag ttcgtcggcg ccctcgccca taaagacgaa ttcgcaaccg ttttcgctgg
     1621 ctagtttcga gagaatgaaa ttcggtatcg aactccttac aaggagcgga tcgaaggatt
     1681 ccagatgata gatcacgtcc ggcagaactt tcagcatctc atcgagatcg tagaaatact
     1741 ccagatgttc ggtacccaga tgatcggcga ccactcttgc gcaccccgga tcttcaccgt
     1801 cgctcttgcc taccgtgaag gtcttgaaat tgccgacaaa ctgcttcaaa gaggccgcga
     1861 tacaactgct gtccagtccg ccgctgagga agatcgaagg ttcgcagtcc agaacttcca
     1921 gtctcttcct tatagaatct tccagggctt ccctcacatc ttcgatggct ttttccttat
     1981 cggtaacggt ctcgccggag tcgatgattt ccctgcaagt ggaatactct ttgaaacctt
     2041 cctctctggt gaagtaatga ccgggcggga agagggcaac ctcgtcgcag taaggtgcga
     2101 tcgccttcaa ctccgaggcg aagatcgtct ctctacctat ccggccgtaa taaagaggct
     2161 tcagccctat tgggtctctg gcgattatcg tctctttgcc atcgttcacg gccagcgaga
     2221 aagagccttc cagtcgctcg agaaagcctt cgccgagctc cctgtaaagc cgcatgacct
     2281 cctccgacca atccgatccc ggcggagaca gaagaaaacc gtcgatgacg gccgcgcctt
     2341 ctggggatga atgtcgcatt tttgaggctg aaaaggccct gccggcggtg gaggccatct
     2401 gaaaaccgtc tacgctttcg tagatagttt tttcttctcc cctgtgagcc atcaagtcta
     2461 gagactcacc aaggagttct tcattgtcac ctgtgaatat gactattgcg ctcatactta
     2521 tctcctttcg agggtagttc tgttggaata taccgaacgc ggctaatatc ggctggattg
     2581 tttatctgct ttaatcgtat tataccatat cgacaatact ggacgctgta accttcattt
     2641 aaagccatat acagaaaaaa cattcgaagg gtgtgaggta gggggtaaga ggtctgaaag
     2701 gtcaagatct gaggatcttc tcacctctcg cctctatcct ctgacctctg acgacgacgg
     2761 ttcccgctct tccgaaggat gaaacgataa gccttcatgt taacgaaaag aatgcgacag
     2821 catcgtcagt ataatatagt gaaaacccgt gatttatgga ggaaaccaac atgagaagca
     2881 tactgatcaa cggagaagta acctataacg cgacatcgca gatattgaac agtaatacct
     2941 tcaagaagat tctatccgag ctaataaagg actccgagga gaagaagaat tcgctccttc
     3001 cccttttcga gccgttcttg aagaacaccg atacagttga caccgcacag aagtacgata
     3061 tagaggggat aatagagcta cttctggctc tcacgatcga tcctatagag aagatcgacg
     3121 gttcgcctta ttactctttc ccgaagatga gcaacaagaa ggatctgctg gtgaggttcg
     3181 tggagagcct cttctctctc tggagaaaca agcacaggtt catcatcaag cgcgacgaat
     3241 atacccccaa caggttcgac agaatattca agcagatgct gctggttaga accaacaccg
     3301 atcttaaagg actcgtactc agtatgtacc gccagatact cataaacgtc tcggatatca
     3361 ggctaaagat cctcagacag gaaccaggcg gcgcacaggt gggtttcatc gttgacaagc
     3421 ccgccgtgga ccccaggatg aagctcaaga acgccgactg gctgtatgaa ttggagtatg
     3481 tctggagtat agtgttcgag ccgccagtga tcttttacac caagtcgaac aagcgcaggg
     3541 gtgtcttcaa ggtcaatgac aggccagttc tcgatagaat cgatatcaca gatcccaaag
     3601 actggttcgg ctttccaata cacgttggaa ccaaacttat atacgtcgtc gtgaacaaag
     3661 agtatctggc tctcgcttcg ggattgggaa atctcttcga gctggccagc ttcgacataa
     3721 taaagaacaa aaagcccgac ggcatataca tactgggtat cgacaatcaa ttcttcgaag
     3781 aacccgacga cttcaacggc gtgatctaca aagagaacga cgggacgtac gtcggcctcg
     3841 tcggagacga tccatccata gactatttcg gctatatgaa gaagatgatc ctcacgattc
     3901 acaacctgct gattatagac gaggggaggc ttccgatcca cggagcgctg gcacatataa
     3961 agttgagaaa cggaaagaag gcaaacgtca tgctcatagg agacagcggc gccggaaagt
     4021 ccgagacgct cgacgctctc aacagacttc agggagaggt atcggaggtg aacatactca
     4081 tcgacgacat gggatcgctg gatatcacgg gcgacgggac ggtagtcgct tacgggactg
     4141 aaaccggcgc cttcgtcagg ctggacgacc tccagccggg gtacgcttac tccacgatgg
     4201 acaggagcat cttcatgaat cccaacgaga taaacgccag ggtgatcgtc ccttactcca
     4261 actacacgga gatcataaaa ccgaccagga tcgattactt tctctacgcc aacaactaca
     4321 acgaagatgt gaaagagaag atcgctttct tcaaaaaggc cgaggacgcc aacgatgtct
     4381 tttctaaagg cgcgcgcatg gccaaaggaa ccacgacgga aaaaggtctg acatacagct
     4441 attttgccaa cccctttggc gccgttcaga gaagagagaa acacgaagcg atcgcgcagc
     4501 gctacctgaa gagtatgata gacggcggtg tcaaggtggg cgaggttatg acccggctcg
     4561 ggattcatgg cttcgaaatg gaaggtccgc ttcaggctgc aaaggctctt ctcgaagcga
     4621 tcgaggaact cgagtcataa aatgtaaaaa taacagtaaa atatgatatc ctttgctacg
     4681 agatcttgtg aaagtggggt gttaacgtgc ctaaaacggt aatcgatgtg agaaatctga
     4741 aaaagcacta cggggaaacg aaggcggttg acgatatcag ctttcaactc atggaaggtg
     4801 aagtcctcgc catactcgga ccgaacggag ccggaaagac tacaactgtt gaaatgctcg
     4861 agggtctcag gaagcccgat tcgggcgctg tcgagtattt cgaagagaaa ctgccccctt
     4921 ccagcgacag ggtgaaggaa gagatcggtg ttctgctgca gtcttctagc ttttttgagt
     4981 acctgtccgt caaggagacg ctcgatcttt tcagggggct ctacaccaaa ggtgtttcgt
     5041 cggatgaatt gatcgaggaa gtttctctcg acgagaagaa aaagacctac acgaaaaatc
     5101 tgtcgggggg ccagcttcag aggctggcgg tagccgtttc tctggtgaac gatccaaagg
     5161 tcgtctttct ggatgaacct acgaccggtc tcgatcctca ggcaaggcga atgctatggg
     5221 aaaccgttct aaatctaaaa aagagacaga aaaccattat tcttaccact cattatatgg
     5281 aagaggccga acatctggcc gacaggataa taatcatgga tcacggaaag ataatatccc
     5341 gcggcactct cgacgaactc atagcttcga tagagactga agatatagtt tctttcagag
     5401 taaagggcga ggaagagatt ccacaggaat tccttcagat ggagggcctc aaacacgtcg
     5461 agaacagaac atacgcggtc actacaaaag acgttgagag aactctggga aggctgttcg
     5521 agagatccaa gaaattcgaa atactgcttg acgacgtaac tataagaaag ccgaatctgg
     5581 aggatgtgtt cataacgctt accggtcgaa aactcaggga ctgagggtga gattcaatga
     5641 gcagaatgtt gaagtttacc tcaggactct tcaaaaacga aatcagggag ttccaggtga
     5701 tgttctggag cttcattttt cccctgattc tctatttcat tctcacgtcg gttttcggag
     5761 gaatggcctc ggggaattcg cagggcatat ctttcaacct gggaattgta aaggaggaaa
     5821 acctcacggg attcggcagg gttctcgacg aagtgctggg aagtataagc tctcatgggg
     5881 gtccctttgt acagagggaa tacggcaatc tggacgaggc cctggaagat ctcaaaaacg
     5941 gtaaacagga tatcgtactc gtgatcccca agggaaccaa cgcaaaaatg acgggatcgt
     6001 tgatgctcca ggttggcgat gtgccgctcc gtatgcatta cataagcgga aaagaatctt
     6061 cgtctattgc ggccaacatc ctgggggaaa tcatgaacga ggtgaacctc gagataaaga
     6121 agcggcagga agagaactat atagacatag cttccacaaa caacgtcgtc tcggccaaac
     6181 aagagaaggg cttcgactac aacgaataca tcttccccgg tgtagcgctg atgatgattc
     6241 tctccgtttc gcttttcaac agccccatcg gattgataca gtacagagtt tccggtacca
     6301 acaagaaact ttacacgact cctttgaagc ctctggagta cttttcggcc catttcgtaa
     6361 agctggtctt cacgatgctc atgtcgctag tgctcctgta catcatcgcg ctcacagttt
     6421 acagggttca gggaactatc ttcgatccgc gattcatcct gtcgctccta tactcgatgc
     6481 ttgtctcggt atcgtttggc ctcatggtgg cctcgttcgc caaaaaactt tcgaccgcca
     6541 ccgtagtggg acagagtctt taccaggtga tgatgttcat gggaggtctc tacttccccg
     6601 tcttcgacct gccctgggga ttgagatgga tagtctatct gctacccacg acgtacctgg
     6661 tggaactcag ccggcgcttc atgggttaca cgatcgcccc gatttccatg acctggttga
     6721 tagtcgttcc cctgatatgg atgatcttct cggtgagcgt cttcgccctg aacttcagga
     6781 aggtgatggg ttatgagtaa aagcaaactg aagccgatgt tcatcgcgtt cgccaaagag
     6841 gctttcagga acaagcttga ggtttttttc acgctgtttt tcccgatgct gtttctgata
     6901 cttttcggct tcttcttcag tggttccggc gggtacagca aatctaagct gggtctcttt
     6961 cagagtggaa cgatggatat aaagccgata atagaggagt caggagcctg gtccgtcacc
     7021 atttacgaga ccagggacaa actgctcgag gccgtcagcg gaggagaggt tcttctgggc
     7081 gtcaccttcg acgggaccgg tattgagtac agctaccggg aaggagactt gagccagaga
     7141 agcaccataa cgatggccca gtcgagcata tcggccgcag ttgaaaagag gatcaacgat
     7201 gccaaaaccg ttatagtcct tgaaaagata cccgagacgg ccggcaggac gacggccacc
     7261 gatgctgact ataccatgtc cggagttatc gccatctcca ttctgtcggc aggaatgttc
     7321 tcggttataa gcgtcttcgg cagatacagg aagcgtgggg tgctcaaaag attcaagatc
     7381 accccggtga aacccatgac cttcattctg ggaagcacac tcacgaggtt tttggtgagc
     7441 ttcgtttcaa tactactcat aatacttgtg agcaaactcc tcttcaagac gacgttccag
     7501 atcgactggc caatattctt gatctcagtg ataagttcga cgctcggcat gatggccctc
     7561 ggtctcttcc taacgcttat cttcaaaaat cccgagacgg ccgattcggc ggcgagcatt
     7621 ctgatggtca taatgatctt ccttgcggga atatacttcc cgctggcttt cctgccagat
     7681 tacctgaggg tagtctcgat gttcatgccg gtcaagtatg tcggcgaatt gatgagacat
     7741 tcactgggaa tagagttgct gccggttatg aacttcgttc tcatcaatct tgtgctttct
     7801 ctgtgtggaa tcgccctgct ttatttcacc ggcaggagat atctcaaggc ttcctgaata
     7861 aatactcaat ctcgcagacc ggaggttata tatcctccgg tctttcttga ttcaagagaa
     7921 atcgcgaccg gtgttgtaga atattaccga cacttttatc tgaaaggggg acaatcatga
     7981 gtcttggaat caaaataacc gacggaatcc actggatagg agtgaacgac tttgaaacac
     8041 acctcttcga ggctctctgg cctctgccta aaggagtgtc ttacaacgcc tatatgatcg
     8101 tggatgagaa agtggttctc atagatacgg ttaagggacc ttacttttcg acttacctcg
     8161 ataaagtaaa atcgttgctc cctgcgggaa agacagttga ctatctggtc gtcaaccaca
     8221 tggaaccgga tcactcgggc tcgataaagg ttttgaggga ggccttcccg gaggtcaaga
     8281 taatcggcaa ccagaaaacg gccgatatgc tgggggcctt ctacggtttg acggacaatc
     8341 tggtcgtagt cagagatgga gaggaactct ccctgggcga a
//
LOCUS       DAOOLY010000090         8355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_9999_length_8355_cov_5.421928, whole genome shotgun sequence.
ACCESSION   DAOOLY010000090 DAOOLY010000000
VERSION     DAOOLY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_8355_cov_5.421928"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1861)
                     /locus_tag="PLD15_06010"
     CDS             complement(131..1861)
                     /locus_tag="PLD15_06010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB63494.1"
                     /translation="MRKIAVFLLALLSYSLVFCGGPEINSATAQEKITLFINGTVVTV
                     DEQMTEAQAVAVKGDRIIAVGTNEEIVKFITEDSEVINLKGKTLMPGFVEVHSHPFVK
                     MVVESAMVDIRPAVGYTDGEKIMDMIEEAVAKAEPGEYLVFFGWDPLLQKGLKNPTKK
                     ELDAIAPNNPLFIWGNSVHIGFANSMAFELAGITKDTPDPVGAMAGTFGRDENGELDG
                     RLDQGGPISMLIKNYLLEYFGGPQQLAEAVYHGWLVNASDGVTTISDNVLEKDILTLY
                     QLTERLQKTLRLRGYAVDYKNIVIPDDSEMIKMVGGKLFIDGSPWTGTISMREPYLVN
                     DTTTGIMDTPAGYMQEPYVTRQELQDFIDDILIRGISAAIHAEGDLAIDWAMDAIEAG
                     LQKYPTADHRIRLEHVPMIRDDQLERAKRLGVAVSFLMAHVRYWGDVIPTLVGEERGQ
                     RWCPVASAVKYDVSYSFHFDGPTSPNKPLESLQTAVTRETVNGRILGPEECISIDEAI
                     RGYTINAAYQLFMDKEIGSIEVGKLADLIILSENPREVDPEKISEIEVLATYINGEVF
                     WSKDSRLIHF"
     gene            2162..2326
                     /locus_tag="PLD15_06015"
     CDS             2162..2326
                     /locus_tag="PLD15_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63495.1"
                     /translation="MSGLPKSCSRIFLNGIIYFGTNHGKIVAVNCSSNGLDGGPWLKY
                     KRDNFNSGRQ"
     gene            complement(2531..3895)
                     /locus_tag="PLD15_06020"
     CDS             complement(2531..3895)
                     /locus_tag="PLD15_06020"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HPB63496.1"
                     /translation="MKTVLKNIEYLVTMNAKREELREAYISIVDGVIEEIGEGAGPAG
                     EVEIDLKGRIVTPGFINTHHHFYQSLFRSVKEVASAKLFDWLVFLYEKWKKLDGEAIF
                     VSSKIAIYEMMASGVTTTTDMLYLHPAGREGFIDSEIEAAKETGVRFHPTRGSMSLSR
                     KDGGLPPDSVVQSERKIIEDSVRLIEKYHDRRRNAMTRIALAPCSPFSVTGESMIATA
                     ELAEKYDVLLHTHLAETKDEDDFCIQKKGLRPVDYMEKVGWLNRRVWFAHLVWLNEKD
                     ISKLVENDCGMAHCPTSNMRLGSGIAPVKEMKAAGMRIGLAVDGSSSNDSGNMLLEIR
                     NALMLQRVLKGADALSPRDVLEFATLGGAKVLRMDDFTGSIEKGKAADIAAFRLDTLS
                     MAGGLSDPVASLVLCDPHGSDFVMVNGKVRVQNGRIIDSDLPELIKRQNHISAKLLEA
                     SQAQ"
     gene            complement(3940..6759)
                     /locus_tag="PLD15_06025"
     CDS             complement(3940..6759)
                     /locus_tag="PLD15_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPB63497.1"
                     /translation="MKLLSVELENFRNHENSLYEFDRGVNLILGKNGSGKSSIIEAIG
                     LALFGGGLRDRQEDAVMWERNRARVVVTFLADDGNQYRAEKVFGSTSNHELRQGELLV
                     ARGRDSVIEKVKTICNLQGDIAKTFENVIVAFQNRIAEQFLGSPAPRREYFNRVFQVD
                     LYRRIATDYMKEYLSGLKSEIALMDGEISLTGKVVEKKSELEKRQEAKLAELANDLKK
                     LEEVESKLTGKKTELETLRSKEREINSLGEKLSILLKDLVEEARRLKESHRQLNTSLR
                     AREITARSFKGHEKYNELLAREKELDGRKNELEVISRKVVEIGRKLAKKTTEQARLQE
                     RTAATERRKGEISESLKTLRERRMEQYGDLEEKRRLLRERELFLQAWNDRKSAAKVLR
                     EEYEKAGRGRIELEAMIKRQSDGLTAQPDPQERLEALDAQEKSLQENIARLESLLAAK
                     AGVSARLKALEESIASLSRGLCPLLNESCKNILERENYAESLERMVQELEDRADKIDR
                     GIEELEGSRQSLEELKKDRARLERDCELKAQAAGELQALREKLERSLERLEKSRRQIE
                     TVLKGSGTPEEMFEALEDGTREAITAVEKARSEVRLSEKGLADIDGELENKLALEVES
                     DRELKKLASAGGELVLELQEMQRESDGFESLLCVLPDLTLALEETRREMNSHKSDYDQ
                     YNRNSVLAGYLEEYTGNFRQSLELCREKSKRIGLSRHEFGELRRGFDRSKLKEVAEEF
                     EGIQEAQRSGRDEVTSIKKDLDYIETELDRIREHEKRLKELKERKALIERKVSLAEKM
                     RENLNLTGEKITAGFRDSIEKKATADYRKISGIEESIIWNEDYEVFLKSPGEAGASLR
                     SFSNLSGGEQVLVSLCLRAAMTNVLSGARLAIFDEPTSNLDRERKELLAQSMRELFGE
                     LDQAIIVTHDDVFSEMAQKVIRLGD"
     gene            complement(6756..7931)
                     /locus_tag="PLD15_06030"
     CDS             complement(6756..7931)
                     /locus_tag="PLD15_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPB63498.1"
                     /translation="MRILRCSDIHLGRRPIGSPGSPYSVARYEDYFKAFENIVNYALS
                     GAVDLFLVSGDLFDRKDINPDTLARTQQMLGRLKEASIDVIAIEGNHDKCFNAGESWL
                     LFLEKTGYLKLLAPFHDGRRYSFPCVETGGVRLFGLGYPGFLVDELLSELAERLEGRN
                     NVVLVHSAPGKNDFIPGLATPEKIEALGEKTLYVAGGHLHARASFPAGEPFFHVPGSP
                     EYWDVWESGEKGFNVFDTLTGEVEFHASSRRERQEFACDVDEELTVDRLNGFVEGLDI
                     HAGSLVLLTVSVPGDCVLYPEKIERRLEEMGALKSFLRVKRRDVPVSLEGFRSMGARE
                     IEREVLTNSREWRELGAKGDSLVRLIDSLKQSQQEGRYEQFKETLDIFLEGLAGESS"
     gene            8068..>8355
                     /locus_tag="PLD15_06035"
     CDS             8068..>8355
                     /locus_tag="PLD15_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPB63499.1"
                     /translation="MSGQTFFFSVLSVWVGFDIYLFLFHNRKVSEKLQEKRSKFVMLA
                     FVLVGMFGAVIVDGKSKEVFLQPFLWYRFMGVGMMIFAVVVRTMVVRELGGA"
BASE COUNT         1664 a         2395 c         2051 g         2245 t
ORIGIN      
        1 atatggtaca aaagaagtgt ccgtgtctcg ctcgcgacca cggaagagcc gaagaaccaa
       61 ccgttcttag aaagtagact gtccagtggc ggctactggc tatttgttta atcctccctc
      121 ttttctttaa ctagaaatgg ataagtcgag agtctttgct ccagaagact tctccgttta
      181 tgtaagtcgc cagcacttcg atctcggata tcttttcggg atctacctct ctggggtttt
      241 cgctcaggat gatcaggtcg gcgagcttcc caacctctat actgcctatt tctttatcca
      301 tgaacaactg ataagcggcg tttatcgtgt agccgcgtat agcttcatcg atcgaaatgc
      361 attcctccgg tccgagtatc cgaccgttca cggtttccct agttacggcc gtctgcaaac
      421 tttccagggg cttgttgggc gaagtcggtc cgtcgaaatg gaacgagtaa ctgacgtcat
      481 acttcaccgc agaagccaca ggacaccagc gctgtcctct ttcttcaccc acgagcgtgg
      541 gaataacatc tccccagtac cgtacgtgcg ccataaggaa gctcacggcc actcccagcc
      601 tcttcgccct ttcgagctga tcgtcgcgga tcatgggaac gtgttcgaga cggatgcggt
      661 gatcggcggt cggatacttc tgcaacccgg cctcgatcgc atccatagcc cagtcgatgg
      721 caaggtctcc ttctgcatgt atcgccgcgg agattcccct tattagtatg tcgtctatga
      781 aatcctgcaa ctcttgccgg gttacgtagg gctcctgcat ataaccggcc ggagtatcca
      841 tgattcccgt ggtcgtatcg ttcaccagat aaggctctct catcgatatc gtcccggtcc
      901 aggggcttcc gtcgatgaaa agctttccgc cgaccatttt tatcatttcg ctatcatcgg
      961 gtatgactat gtttttgtaa tccaccgcgt agccgcgcag acgaagagtc ttttgcagcc
     1021 gctccgttaa ctgatacagg gtcagtatat ctttctcgag aacgttgtcg cttatagtgg
     1081 tgactccgtc gctcgcgttg accagccacc catggtaaac ggcctctgcc agctgctggg
     1141 gacctccgaa atattcgagc aggtaattct ttatcaacat ggaaatcgga ccgccctgat
     1201 cgagccttcc atcgagttcg ccgttctcat cccttccgaa ggttcccgcc atagcgccga
     1261 cgggatctgg tgtgtctttg gttatacccg ccagctcgaa agccatcgaa tttgcgaaac
     1321 ctatgtggac gctgtttccc cagatgaaaa gagggttgtt gggcgctatc gcatccagtt
     1381 ctttctttgt ggggtttttt aaaccctttt gcaggagagg atcccagccg aagaagacca
     1441 aatactcacc tggttcggcc ttcgcgacgg cttcttcgat catgtccatg atcttctcgc
     1501 cgtcggtata acccacggcg ggccttatat cgaccatggc gctttcaacg accattttga
     1561 cgaaaggatg agaatgaacc tcgacaaatc ccggcataag ggttttgccc ttcaaattga
     1621 taacctcgga atcttcggtg atgaatttga cgatttcttc gttcgtaccg acggcgatga
     1681 ttctgtcgcc cttaacggcc acggcctgcg cctcggtcat ctgttcatcc acagtcacca
     1741 ctgttccatt gatgaacaga gttatctttt cttgagcggt ggcggaattg atttcgggtc
     1801 cgccacagaa aaccaatgaa tacgacagca gtgcaagcag aaaaacggct attttcctca
     1861 ctgggttcac ctcccttttc aggtccctta gatctctgtt tttgaagtat ctttcgcaca
     1921 aacatgccac acgagtgaac tagacaaaga acatggatta gcaaccggaa agccttgcgt
     1981 tcaaagtccg cagaaagaac tgattctttt ttcaaagagc cggtatgaat ctcgaaaaca
     2041 ggcaaactca tatgaatcgt aagtttgaaa ctaattatag cacgagaaat cgccattaca
     2101 gccttgccag caggcttctt cacttgaaat cataagttga gataactgaa gagtttgaga
     2161 tgtgtctgga ttgccgaagt cttgtagtag aatatttctt aacggaataa tctatttcgg
     2221 caccaaccac ggcaagatcg tcgccgtcaa ttgcagcagt aacggcctgg atggcggccc
     2281 ctggttgaag tacaaaagag acaacttcaa ttccggaaga cagtaattgg gaagaggaga
     2341 gagaaagaga gagaaagaga gagagagaag gagagagaaa gagagagaga aagagagagg
     2401 aaaaaatggg gacagacctc aggaaacagt cagtccccat tttcgcgaga gaacagaggt
     2461 gagaggtgtg aagagcgtga agatcttgat cttcttacct cttacctctc ggttcttgcg
     2521 cacccattac tcattgtgcc tgactcgctt ccagcagttt tgccgatatg tggttctgtc
     2581 tttttatgag ctcgggcagg tcgctgtcta tgattcttcc attctgtacg cgcactttgc
     2641 cgttgaccat cacaaaatcg cttccatgag ggtcgcagag aacgagcgag gcaaccgggt
     2701 cggagagccc gccggccatg gacagggtgt ccagtctgaa agcggcgata tcggcggcct
     2761 ttcccttttc gatcgagccg gtgaagtcgt ccattctcag gactttcgct ccgccgagcg
     2821 tggcgaattc gaggacatct ctgggagaga gcgcgtcggc ccccttcaaa accctctgga
     2881 gcataagcgc gtttctgatt tcgagcagca tgtttcctga gtcgttcgaa gagctgccat
     2941 ccacggccag acctattctc atgccggccg ccttcatctc ctttaccggc gctattcccg
     3001 aaccgagacg catgttagag gtcgggcagt gggccattcc acagtcgttt tcaaccagct
     3061 tcgatatatc tttttcgttg agccacacca ggtgggcaaa ccagacccgt cggttcagcc
     3121 agccgacctt ctccatgtaa tctaccggcc gcaggccctt cttttgtatg cagaaatcgt
     3181 cttcgtcttt cgtctcggcc aggtgagtgt gcaagagcac gtcgtacttt tcggccagtt
     3241 cggccgtcgc gatcatcgat tccccggtga ccgagaaggg cgaacagggg gccagcgcaa
     3301 ttctggtcat cgcgttcctc cgcctgtcgt ggtacttttc gatcaggcgc accgagtctt
     3361 cgattatctt tctttcgctc tggaccaccg aatcgggggg gaggccgccg tccttcctgg
     3421 agagagacat cgagccgcgg gttggatgga atctgacgcc ggtctctttt gccgcttcga
     3481 tttcactgtc gatgaagccc tccctgccgg ccgggtggag gtagagcata tcggtggtgg
     3541 tcgtgacgcc ggaggccatc atctcgtata tggcgatctt cgagctgacg aagatggctt
     3601 ctccgtcgag cttcttccat ttttcataga gaaagacaag ccagtcgaac agcttggccg
     3661 aagcaacttc tttgacgctg cgaaagagag actggtagaa atggtggtgg gtatttatga
     3721 agccgggagt cactatgcgc cccttgaggt cgatttccac ctcgcccgcc ggaccggccc
     3781 cctcgccgat ctcctcgatc actccgtcca ctattgatat ataagcctct ctaagctctt
     3841 cccttttcgc gttcatggta acgagatact ctatgttttt cagtaccgtt ttcaaagctc
     3901 acacctccgt tcggtggtta tacgatttca atctacggcc taatcgccca gccggataac
     3961 tttttgtgcc atctcggaaa agacgtcgtc gtgcgtgact attatcgcct ggtcgagctc
     4021 gccgaaaagc tccctcatcg attgcgccag cagttccttt ctctccctgt cgagattgga
     4081 agtgggctcg tcgaagatgg cgagtctcgc accgctcaaa acattggtca tggccgccct
     4141 caggcagaga gagaccagca cctgttcgcc gccggataag ttcgaaaagc ttctcagact
     4201 cgcgcccgcc tcgccgggag atttcaggaa aacttcgtaa tcttcgttcc agatgatcga
     4261 ctcctcaatt ccggagatct tcctgtaatc tgccgtggcc ttcttctcta tactgtccct
     4321 gaagccggcg gttatcttct cgcccgtgag gttcaggttc tctctcatct tttccgcgag
     4381 cgaaaccttt ctctcgatca aggcctttct ctctttcaat tctttcaagc gtttctcgtg
     4441 ttcgcgtatc ctgtcgagct ccgtttctat gtaatctagg tctttcttta tagaggtcac
     4501 ttcgtcccgt ccggaccttt gggcctcctg tatcccttcg aactcctctg cgacttcctt
     4561 caatttcgac cggtcgaagc ccctgcgcag ttcgccgaat tcgtgtctcg acagccctat
     4621 cctcttcgat ttctccctgc agagctccag agactggcgg aagttcccgg tgtattcttc
     4681 gagatagccg gccaggacgg agtttctgtt gtattggtcg taatcgcttt tatgagagtt
     4741 catctctctt ctcgtctctt cgagggcgag ggttaagtcc ggaaggacgc acagcaagct
     4801 ctcgaaaccg tcgctctctc tttgcatctc ttgaagctcc aggacaagtt ctcccccggc
     4861 tgaggccagt ttcttcaact ctctatccga ttccacttcc agagccagtt tgttctcgag
     4921 ttccccgtcg atgtcggcca ggcccttttc ggacaggcgg acttcgcttc tggccttctc
     4981 gacggccgtg atggcctctc tcgtcccgtc ttccagagct tcaaacatct cttcgggagt
     5041 tccgcttccc ttcaggaccg tttctatctg ccttctggat ttttcgagtc gctccagcga
     5101 tctctccagc ttctctcgaa gggcctgcag ttctccggcg gcctgtgctt tcagctcaca
     5161 gtctcgctcc agcctggccc tgtctttctt cagttcttcg agcgattgac ggctcccttc
     5221 aagctcttcg atccctcgat cgatcttgtc ggctctgtct tccagttctt gaaccattct
     5281 ctccagactc tccgcgtaat tttctctctc caatatgttc ttgcaacttt cgttgagcaa
     5341 ggggcagagg ccccttgaca gagaagcgat agattcttcc agagccttca gcctggccga
     5401 gacgccggcc tttgctgcta gcagcgattc gagccgggcg atgttttcct ggagcgactt
     5461 ctcctgcgca tccagtgctt cgagccgctc ctgtggatcg ggttgggccg tcagaccatc
     5521 gctctggcgt tttatcatcg cttcgagttc gatgcgcccg cgcccggcct tttcgtattc
     5581 ctccctgagc actttcgcag cgctctttct gtcgttccag gcctgcagga acaactccct
     5641 ttctctgagc agtcgccgct tttcctcgag atcgccgtac tgttccattc tcctctctct
     5701 cagagttttc agcgactctg agatctcgcc tttgcgcctc tccgtcgcgg cggttctctc
     5761 ctgcaatctg gcctgttcgg tggttttctt cgcgagcttt ctgccgattt cgaccacttt
     5821 cctcgagatg acttccagtt cattctttcg cccatccagt tccttctccc tggccaggag
     5881 ttcgttgtat ttctcgtggc ccttgaagga tctggcggtt atctcgcggg cccgcaagga
     5941 tgtattgagc tgtctgtggc tttctttaag ccttctggcc tcttcgacga ggtctttcag
     6001 cagtatcgag agtttctctc caagcgaatt tatctccctt tctttcgatc taagggtttc
     6061 aagctcggtt tttttgcccg taagtttact ttctacctct tccagttttt tcaggtcgtt
     6121 cgccagctca gcgagtttgg cttcctgtct tttctcgagt tcggatttct tttccacgac
     6181 ctttcccgtg agcgaaatct ctccgtccat gagagctatc tccgacttca gaccggagag
     6241 atactccttc atgtaatcgg tcgcgatacg tctgtacaga tcaacctgaa agacgcggtt
     6301 gaaatactct cttcgcgggg ctgggcttcc gaggaactgc tccgcgattc tattttgaaa
     6361 ggcgacgatg acgttttcga aggttttcgc gatatcgccc tggaggttgc agatggtctt
     6421 aactttttct atcacggaat ctctgcccct ggccacgagc agttcgccct gccgcagctc
     6481 gtggttcgag gtgctgccga ataccttttc ggcccggtac tggttcccgt cgtcggcgag
     6541 aaaggtcacc acgaccctgg ccctgtttct ttcccacatt accgcgtctt cctgccggtc
     6601 tctgagacct ccgccgaaaa gcgcaagccc gatcgcctct atgatcgagg atttgccaga
     6661 gccgttttta ccgagtataa ggttgacgcc cctgtcgaat tcgtaaaggg agttttcgtg
     6721 gtttctgaag ttctccagtt ccacgctgag cagcttcatg aactctcacc cgccaatccc
     6781 tccaggaaga tgtccagagt ctctttgaac tgttcgtacc gtccctcttg ctgagactgt
     6841 ttcagcgaat cgatcagcct gaccagcgag tcacccttcg cacccagctc tctccattct
     6901 ctggagttcg tcagcacctc gcgttcgatc tcccttgcgc ccatcgagcg gaacccttcg
     6961 agcgagacgg gaacatccct tctcttgacg cgaaggaagg atttgagggc ccccatctcc
     7021 tccagtcttc tttcgatctt ctcggggtac agcacacaat cgcccggtac gcttaccgtg
     7081 agcaggacga gcgaacctgc atgtatgtcc agcccttcga cgaaaccgtt cagtcgatcc
     7141 acggtcaact cttcgtcgac gtcgcaggcg aactcctgtc tctctctccg cgaggaggca
     7201 tgaaactcca cctctcccgt aagcgtgtcg aatacgttga agcccttctc gccgctctcc
     7261 cagacatccc agtattcggg cgaaccgggc acgtggaaga agggctctcc tgcagggaag
     7321 ctggccctcg catggagatg gccgccggca acgtacagcg tcttttcccc gagcgcttcg
     7381 atcttctccg gcgtggccag ccccggtatg aagtcgttct ttcccggcgc cgaatggacg
     7441 agaactacgt tgttcctgcc ctccagcctt tcggccagct ccgatagcag ttcatccacg
     7501 agaaatccgg gataacccag gccgaagagc ctgacccctc cggtttcaac gcatggaaag
     7561 gagtagcgcc ttccgtcgtg aaagggtgct agaagcttca aatagcctgt cttctccaga
     7621 aagagcagcc aggattcgcc ggcgttgaaa catttatcgt ggttgccctc gatcgcgatc
     7681 acatctatcg aagcttcttt gagcctgccc agcatctgct gggttctggc cagcgtatcg
     7741 gggttgatat ccttcctgtc gaagagatcc ccgctcacca gaaagagatc gaccgctccc
     7801 gacagagcgt aattgacgat gttttcgaag gctttgaaat aatcctcgta ccgggccacc
     7861 gagtaagggc tgcccggaga gcctatcggt ctcctgccga gatggatatc cgaacagcgt
     7921 agtattctca accaatcacc accttcaaga caatattact ataactcttc gcgcgaatag
     7981 agagatttca cacgatcgtc ggtcgatggt atcgttcatg ggactgatga tagaatcttc
     8041 atatgttatg gattccagga ggtggcgatg tccggtcaga cctttttctt ctcggtcctc
     8101 tcggtatggg tagggttcga tatatatctc tttctcttcc acaacagaaa ggtcagcgaa
     8161 aaacttcagg aaaagcgctc caagttcgtc atgctggctt tcgttctcgt cgggatgttc
     8221 ggcgccgtaa tcgtcgatgg aaaatccaag gaagtctttc ttcaaccctt cctctggtac
     8281 aggtttatgg gcgtcggaat gatgatcttc gccgttgtgg tcaggaccat ggtcgttcgc
     8341 gagctcggcg gggcc
//
LOCUS       DAOOLY010000091         8291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10114_length_8291_cov_4.787518, whole genome shotgun sequence.
ACCESSION   DAOOLY010000091 DAOOLY010000000
VERSION     DAOOLY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10114_length_8291_cov_4.787518"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..629
                     /locus_tag="PLD15_06040"
                     /pseudo
     CDS             40..629
                     /locus_tag="PLD15_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
     gene            645..2066
                     /gene="atpD"
                     /locus_tag="PLD15_06045"
     CDS             645..2066
                     /gene="atpD"
                     /locus_tag="PLD15_06045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPB63500.1"
                     /translation="MPDKKTTNAGKIVAITGPVVDVSFEGAALPDILNALEVENKFLK
                     KKIVLEVSQLIGDNSVRAIAMDSTDGLVRGQEVIDTGGQITVPVGEEILGRMFNLLGE
                     PIDELGDVEFKDRWQIHREAPPVTEQQTEIEILETGIKVIDLLAPFSKGGKIGFSGGA
                     GVGKTVLVMELIRNFAIEQKGLSVFAGVGERTREGNDLWLDMKEAGVIENTALVFGQM
                     NEPPGARFRIGLSAITMAEYFRDVQKKDVLVFIDNIFRFVQAGSEVSALLGRMPSAVG
                     YQPTLATEMGKLQERITSTRSGSITSVQAIYVPADDFTDPAPATTFSHLDATVNLSRS
                     IAEQGLYPAVDPLDSTSKNLDPVVVGSEHYEVARKVQEVLQRYKDLQDIIAILGMEEL
                     SEEDQLTVQRARKIQRFLTQPFFVAEKFSNIQGKYVPIAETVRGFREILDGKHDDLPE
                     QAFMMVGTIEEAVEKAKILTAGE"
     gene            2068..2466
                     /gene="atpC"
                     /locus_tag="PLD15_06050"
     CDS             2068..2466
                     /gene="atpC"
                     /locus_tag="PLD15_06050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPB63501.1"
                     /translation="MPLKTSIVTPYGIVWEGDAEYISFKSVEGEIGFLPERAPAIMKL
                     VVDVVEIRAIDGVHTFAVHGGYILQERDKTTIITDAAERPEEINVERAKQRLKRAEEL
                     LEISNSRRERARNQAKLQRHMLRIKLGSER"
     gene            2615..3526
                     /locus_tag="PLD15_06055"
     CDS             2615..3526
                     /locus_tag="PLD15_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB63502.1"
                     /translation="MLEQDHTGDGILDRAIKLERVLESLPDPSAIISSTGNILHGNSG
                     WERLASSLALESATFMESGKEFGRLVKRFAEEKKLEEFKDLLSGRRSSLNFELNLFEV
                     PEGRGFSLFVSRVALQDQPIFLAVVKDGTEINNLRCLLKEIIYRDNLTGLENENSFNL
                     YLSGRIHRAMNAGSSLFVLYIEIGNIKEITAMFGKRISNSLIVRICKRLRENLGGREF
                     FRVQHDAFAAVFESIDRGILYLELSSLIGKLEKKIICENLELVPDILAGVCEFPVDGR
                     NVDDILSNCLGALFRAKRENLKWSFYS"
     gene            complement(3532..5670)
                     /locus_tag="PLD15_06060"
     CDS             complement(3532..5670)
                     /locus_tag="PLD15_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63503.1"
                     /translation="MRRLFLIGLVAITSMVVITFALKEKTEIKVLDTPSLSVKIGEEL
                     RVNLSDFISGPNGPVNFKIIKGSGKIEGSIYTVSPRGLEDCEDVVEIVVEAGKSEAVI
                     TLNIEILNDPKAPSIEIGNQKVYEGETLMINLPDFTSADSSDVTYELVSGVGSIDEGV
                     YSYLPGSTEAPTAHRVTIKAMDGRGEWQCTTFSVIVIDVNNWPGEPANPFPADGTVDF
                     FREVTLLWSAEDPDGDDLFYDVYFGEKGELELLAKNIEENEWTISFLEAGREYFWRIH
                     ARDGRGGEVQGPLWSFSTKEIPSIFWKRLLGGSGRDSVNSLKALTDSTFVMTGWTESH
                     ELPKWRSEEDVFRSGWVVKLDSNGYLVWQKFLDFGWTEISDIIATEDGGFLLAGRSST
                     GNGFTSKEPSNLLLVSLDRFANVKWKTELGRTVSSEVSLAKADDGFLVLGTAAGSEEI
                     SRGDVLLIKLDLDGRPVWEKKYGGSSYERAIDMAIAGNGDIVIAAETTSKDGDSNGNV
                     SRVLKVNGIEIENSSVLVLRVDCSGGVLWTKVFGGIGEDSPAAVEIGPAGEIFVCGST
                     NSQSGDFKRKSGDYDGFIIKLDGDGELIWKKIYGGRSNDYLTDIAITGSGGAIVVGFT
                     ESDDVTLGSFHEGELDKLGLTYSDMWVFQVSRSGEILWSDCYGGTLGDVAMAISLCDD
                     GFAITGYTASEDGDIPINRGSFDIITLRFK"
     gene            complement(5675..7954)
                     /locus_tag="PLD15_06065"
     CDS             complement(5675..7954)
                     /locus_tag="PLD15_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPB63504.1"
                     /translation="MNIPAKVIELRRLLLVVLFVFFIWTTFFSVEYIPDETAFSINCI
                     ALTPDGKNLVAGYIDNTVKVWNLLDGKLVMNLLFHSSWVTSVTVSPDGRLGIAGYDDG
                     MIRVWEMETGKPLKSLSSHTSSVNYLLVSKDGKTLYSAGSDGTVKVWSLSELAFIRSL
                     RGHSGPVNSIVHWPEQNRLYSVSDDGTLRIWDTSTGQQVKMIKAEIGALSSVTMDEER
                     SILIVGSRDGPLKFYDVKTWSALRIVKAHNAEVTAIIIRDDLMFTSSCDRTVKILSLP
                     SGSLVKMITGHSWDVSSIILSSDSSVIYSGSSDGAVKIWDVDKGAALGTLIGFGDGEY
                     FSYNSDGNWVASSKGASRVKTSSGKRPDQQGTELPSLLLQLDKLPRIYIQSPQSITIY
                     DTDLTFVVSQPVVKVTVNDEEVPVGESGEVTFKTTSAGVLTVKAFDSTGNFDEKILEV
                     QFEETRMYVVGNIGPYRRGDRVVVSDRNATDLLVNGEWLSRGFFSDSAPDIEPPQVIG
                     QISQQVKIGQEKYLEFVVTDNDEVETVIVIGPDEKREEVSVNSPHESMRIKVGGTGEY
                     HVTAIDNEGNEIEVTLSLIASAKIFWVERDHGSLRRGQEVTVTAEGESSFFVREYGWV
                     EKDILTDTKITTESPVIVGEDVQYAEADKEKMLSFKVSDDIKVIEVEVSEKIYPVDLK
                     EKEMFVIVGDYGEYRVVARDLEGNTSEKTIRLAAPAKGEKKTNWLLVSVAVATVVILL
                     LLIFRPFFTRKKKRKVRLM"
     gene            complement(7996..>8291)
                     /locus_tag="PLD15_06070"
     CDS             complement(7996..>8291)
                     /locus_tag="PLD15_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPB63505.1"
                     /translation="FYRDFLGLELFKDQGSCRIYSVPSGGMIGFCEHLPVEAGMFSPI
                     VTFLTEFVDDFYEKALRLEMSPNKPSVNERFGIYHFFLKDPDGHRVEIQKFLD"
BASE COUNT         2216 a         2051 c         1879 g         2145 t
ORIGIN      
        1 agcggtgaag gtgacttgct actggtagta acggccgaca tgggattggc cggggccttc
       61 aacgccgaaa tactcaagct cgcggaacaa gaattgaaga agctgaacgt gaagcgactt
      121 gtaaccgttg gggtgaaagc agaaagctat ttcaaaaagc atggtgtcga actttccgct
      181 ttcagtcatt tctatgatac tccagatctt gatgaagccg cgattatcgt ggacgatatc
      241 atggacgcta tggaggccaa taacctcggt ggtttaagca tggtttacag caacttcaaa
      301 acgcctcttg cgcaggtacc caaagtagtg aatatactgc ctctgaaacc cttcgaagaa
      361 cggagaacga tctctacgat tttgagcctg acatagaact tctgtaccgc gatattctgt
      421 attcctggat agtatcgatc gtgcttcgag gtctttacga gaccaaggta ggcgagctgt
      481 atgccagaca gaacgccatg aagaatgcca cagagaacgc cagtaatatg atcgaaagac
      541 tcacactcgt gagaaacaaa cttcgccagt cgaacataac ccaggaaatc atagaggttg
      601 tgaacggtgc cgaagctctt aacgggtaat ctggaggtgt aagtgtgccc gataaaaaaa
      661 caactaacgc gggtaaaata gtcgccataa cggggccggt agtggatgtc tcatttgaag
      721 gtgccgctct tcccgatata ctgaacgctc tggaagtgga aaataagttt cttaaaaaaa
      781 agatcgtact tgaagtgtcc caactcatag gtgacaacag tgtcagagcg attgccatgg
      841 acagcaccga cggtcttgtg agaggacagg aggttataga tactggtggc cagattaccg
      901 tacctgtggg cgaagagata ctgggaagaa tgtttaacct tctgggagag ccgatagacg
      961 aactcggcga tgtagagttc aaagacaggt ggcagataca cagagaggct ccgccagtta
     1021 ctgagcagca gacagagatt gaaatactgg agaccggtat caaggttata gatctactgg
     1081 cgccttttag caaaggcgga aagataggct tttcaggtgg cgccggggtg ggaaagactg
     1141 tccttgttat ggaactcatc cgcaacttcg ccatagagca aaaaggactc tccgtattcg
     1201 ccggggtcgg agaacgtacg agagagggga atgatctctg gctggacatg aaagaggccg
     1261 gtgtcataga aaataccgcg ctggtttttg gccagatgaa cgaaccgccg ggagcacgtt
     1321 tcaggatagg cctctccgct ataaccatgg ccgagtattt cagagatgtt cagaagaaag
     1381 atgttctggt tttcatagac aacatattca ggttcgttca agccggttcg gaagtttcgg
     1441 ccttacttgg aagaatgccc tcggcagtcg gttaccagcc gacgctggcc acagagatgg
     1501 gaaagcttca ggaaaggatt acctcgacga gaagtggctc gatcacctcc gttcaggcga
     1561 tatacgttcc ggcggacgac ttcacggatc cagcgcctgc aactacgttc agccatctgg
     1621 atgccaccgt caacctttca agatcgatcg cggagcaggg actctacccg gctgtcgatc
     1681 cactggattc gacatctaaa aacctcgatc ctgtcgttgt tggaagcgaa cattacgaag
     1741 ttgcaagaaa ggttcaagaa gttctccaga ggtacaagga ccttcaagat ataatcgcaa
     1801 tacttggaat ggaagaactc tcggaggagg accagctaac tgtgcagaga gccaggaaga
     1861 tacagcgttt ccttacgcaa cccttcttcg ttgctgagaa gttcagcaat atacagggta
     1921 agtatgttcc gatagccgag accgtgagag gtttcaggga gatacttgac ggcaagcacg
     1981 atgatctacc ggaacaggct ttcatgatgg tcggaaccat agaggaagcc gtggaaaagg
     2041 cgaaaatact gacggcggga gaatgatatg ccgttaaaga caagcatagt gacgccttac
     2101 ggtattgtct gggaaggaga tgccgaatac atatccttca agtctgtaga gggtgaaatc
     2161 ggtttcctgc cagaaagggc gccggcgata atgaagcttg tcgtcgatgt ggtagaaata
     2221 agagccatcg acggagttca caccttcgcg gtacacggtg gatacatact tcaggaaaga
     2281 gataagacga cgattattac cgacgcggcc gaaaggccag aagagatcaa tgtcgagcga
     2341 gcaaaacaaa gattaaagag ggctgaagag cttcttgaga taagcaactc cagaagagaa
     2401 agggcaagaa atcaggctaa attacagaga catatgctcc gaataaagct aggttcggag
     2461 aggtagcaag agccgaaagc atagtttgaa ccgcttatta ccggggatat gttgattgta
     2521 tctccggttt ttcttattta ttgatctttg gcattgattt gcataaacaa tcaattggta
     2581 agctcataat agttaaccgg gagaagggac agatatgctg gaacaggatc acaccggaga
     2641 cgggattttg gatcgggcga taaaactcga gagggttctt gaaagccttc ccgatccgtc
     2701 ggccattatc tcttcgacag gaaatatcct gcatggtaat tctggctggg agagactggc
     2761 ttcctctctc gctctggaat cggctacctt tatggagtcg ggtaaagaat tcggaagatt
     2821 ggtgaaacgt tttgccgaag agaaaaagtt agaagaattc aaagatcttc tctcggggag
     2881 aagaagctct ctaaacttcg aacttaacct ctttgaggtt cctgagggca ggggtttttc
     2941 actgttcgtt tcacgcgttg ctcttcaaga ccagccaata tttctcgcag ttgtaaaaga
     3001 cggtactgaa atcaacaacc tccgctgctt gcttaaagaa atcatatacc gtgacaattt
     3061 gacaggacta gaaaacgaaa actctttcaa tctatatcta tctggcagaa ttcatcgggc
     3121 gatgaacgcg ggcagctctc tcttcgttct ctatatagag atcggtaata taaaagagat
     3181 aacggccatg ttcgggaaac gcatttccaa ttctcttata gtgcggattt gtaaaagatt
     3241 gagagagaat cttggaggta gagaattttt cagagttcaa cacgatgcct tcgctgcggt
     3301 cttcgagtcg attgacaggg gaattctcta tcttgaattg agctctctta taggaaaact
     3361 tgagaagaaa attatctgtg aaaacctcga actggtcccg gatatcctgg ctggtgtttg
     3421 cgaattcccc gtagacggca gaaatgttga cgatatcttg agtaactgtc ttggcgcact
     3481 gttcagggcg aaaagagaga accttaagtg gagtttctac agttaaaact gctacttgaa
     3541 gcgaagggta atgatgtcga agctgcctct gttaattggt atatcaccgt cttctgaggc
     3601 cgtgtagccg gttatcgcaa aaccatcgtc acacagggaa atcgccatgg cgacatcgcc
     3661 aagagtccct ccgtaacaat cgctccagag aatttccccg gaacggctta cctggaagac
     3721 ccacatatcg ctgtaggtaa gtcccaactt gtccagttct ccctcgtgga aacttcccag
     3781 cgtaacgtcg tccgattcag taaaacctac cactatggcc cctccagagc cggtaatggc
     3841 tatgtcggtg agataatcgt tcgagcgacc tccgtagatt ttcttccaga ttaattctcc
     3901 atcaccgtcg agttttatta tgaatccgtc gtagtctcca ctcttccttt tgaaatctcc
     3961 gctctgcgaa tttgttgaac cacacacgaa aatttcgcct gctggaccaa tttcgacggc
     4021 ggccggtgag tcctctccaa tacctccaaa gaccttcgtc catagaactc cgccactgca
     4081 gtcaactcta agaacgagta ccgaggaatt ctcaatctcg atcccgttga ctttcaagac
     4141 tctcgaaacg ttaccgtttg aatcgccgtc tttagaagta gtttcagccg ctatcactat
     4201 atctccgttt ccggctatag ccatatctat tgcccgctca taggagctac ctccgtactt
     4261 cttctcccag acaggtctac cgtcaagatc taacttgata agcaacacat cgccgcgtga
     4321 tatttcctct gaaccggccg ctgttccgag taccaggaag ccgtcatctg cctttgccag
     4381 agaaacctca gagctgactg ttcgcccgag ttcggtcttc cacttcacat tggcaaacct
     4441 gtcgagactt accagcaaca agttcgaagg ctctttgctt gtgaaaccat ttcctgtcga
     4501 gctcctgccg gctagaagga agccaccgtc ctctgtagct attatgtccg atatctcggt
     4561 ccagccgaaa tccagaaact tctgccagac taaatagcca ttactatcca gtttgactac
     4621 ccagccgctt ctgaagacat cttcctccga cctccatttt ggcaattcgt gagattccgt
     4681 ccagcctgtc ataacgaaag tgctatctgt aagtgctttg agtgagttaa ccgaatcacg
     4741 accactcccg cccagaaggc gcttccagaa aatggagggt atctcttttg tagaaaaact
     4801 ccacagcggg ccttgaacct ctccacccct cccgtccctc gcatggattc tccagaagta
     4861 ttcacgtcct gcctccagaa aggaaattgt ccactcattt tcctctatat tctttgcaag
     4921 aagctccagc tcacctttct ccccgaaata gacatcgtaa aaaaggtcgt cgccatcggg
     4981 atcttcagcg ctccagagga gtgtgacttc tctaaaaaaa tcaacggttc catcggcagg
     5041 aaaggggttt gccggctctc cgggccagtt atttacatct atgactatca cggagaatgt
     5101 tgtgcactgc cactcccccc gtccatccat cgcctttatt gtgacacggt gtgcggttgg
     5161 cgcttctgtg gatccaggga gatacgaata caccccttca tctatactgc caactccgct
     5221 cactagttcg taggtgacat cgctggagtc ggctgaagtg aaatcgggaa gatttatcat
     5281 caaagtttca ccctcgtaaa ctttctggtt gccgatctct atagagggag cctttggatc
     5341 atttaagatc tctatgttca gtgtgataac tgcttcgctc tttccagcct ccaccactat
     5401 ctcaaccaca tcctcgcaat cttccagtcc cctgggagaa actgtataga tacttccctc
     5461 tatctttcca ctgcccttga tgatcttgaa attgacaggt ccgtttgggc cggagataaa
     5521 atctgacagg tttactctca gctcttctcc tatcttcaca ctcagcgaag gcgtatccag
     5581 aaccttaatc tcggtctttt ctttcagagc aaaggttatt accaccatcg atgtgatcgc
     5641 taccaggcct atgagaaaaa gccttcgcat agtctcacat cagccttact tttcgcttct
     5701 tctttctagt gaaaaaaggt ctgaatataa gtaaaagaag gatgactacc gtggcaactg
     5761 caaccgacac cagtaaccag ttggtttttt tctcgccttt tgccggagct gccagcctta
     5821 tggttttttc ggaagtgttg ccttcgagat ctctggcaac gactctgtat tcgccataat
     5881 ctcccacaat aacgaacatt tctttttctt tgaggtccac cggatatatc ttttcagaaa
     5941 cttctacttc aattaccttt atatcgtcgc ttactttaaa gctcagcatc ttttccttat
     6001 cggcctcagc gtattgtaca tcttcgccca ctataacggg gctctcggtg gtaatcttcg
     6061 tatcagtcag tatatctttc tcgacccagc cgtattccct aacgaagaag gaactttcac
     6121 cctcggccgt aacggttacc tcctggccgc gtctgagact tccgtgatct ctttccaccc
     6181 agaagatttt tgcacttgca atcagtgaaa gggtcacttc gatttcattt ccctcgttat
     6241 ctatagccgt tacatggtac tcaccggtgc caccgacctt gattctcata ctctcgtgtg
     6301 gggagtttac agacacctct tcgcgtttct cgtcaggtcc aataacgatc accgtttcaa
     6361 cctcatcgtt gtcggtcaca acgaattcaa ggtacttttc ctggccgatc ttaacctgtt
     6421 gcgatatttg ccctattacc tggggaggtt ctatatccgg tgcgctatcg gaaaaaaaac
     6481 cccgcgaaag ccattcaccg ttaaccagaa gatccgtagc attacgatcg ctcacgacca
     6541 cccgatcgcc ccgcctgtac ggtccgatgt taccgaccac atacatcctg gtctcttcaa
     6601 actgaacttc caatatcttt tcatcgaaat tccctgttga atcgaaggct ttgacggtta
     6661 atacaccggc cgaagttgtc ttgaaggtca cttcgcccga ctctccaaca ggaacttcct
     6721 catcgttcac ggtgactttc acaacgggct gtgaaacaac gaaagtcaag tcggtatcgt
     6781 atattgtaat tgattgtgga gattgaatgt atatcctcgg gagtttgtcc agctggagca
     6841 atagactggg aagctccgtt ccctgctgat caggccgctt tccagaagag gttttgacac
     6901 gcgaagcacc tttactcgaa gcaacccagt taccatcgct gttgtaggag aagtattccc
     6961 cgtcgccgaa acctatcaag gtaccgagag cagcgccttt atcaacatcc catatcttca
     7021 cagcaccgtc acttgaaccg gaataaataa cgctagaatc gctcgataga attatcgaag
     7081 aaacgtccca ggaatggcct gttatcattt ttacaagaga gcctgacggt agagaaagta
     7141 tcttcactgt tctatcgcag gaagaagtga acattaaatc atctcttatg attatcgccg
     7201 ttacttccgc gttgtgagcc ttcactattc tcaatgccga ccatgttttt acatcataga
     7261 acttcagagg tccgtctctg gagccaacta tcagaatgct cctctcctca tccatcgtga
     7321 cggacgagag agctccgatt tcggccttaa tcatcttcac ctgttgacct gtagaagtat
     7381 cccaaattct caatgtacca tcatcagaca cagaatataa gcggttctgt tccggccagt
     7441 ggactatcga gttgaccggt ccagagtggc ctctcaacga tcttatgaag gccagttcag
     7501 atagcgacca caccttgact gttccgtcgg aaccggccga ataaagagtc ttaccgtcct
     7561 tcgagaccag caaataattc actgaactcg tatgactgct cagcgatttt aaaggtttac
     7621 cggtctccat ctcccagact cttatcatac catcatcgta tccagctata ccaagccttc
     7681 cgtctggcga gactgtaacc gaagtcaccc aggagctgtg gaataatagg ttcatgacta
     7741 gctttccatc cagtaagttc cagaccttca ccgtgttatc tatatagccg gccacaagat
     7801 tctttccatc gggtgttagt gcgatgcagt ttatgctgaa agccgtctcg tcgggaatgt
     7861 actccactga aaaaaaggta gtccaaatga aaaaaacgaa caaaacaaca agtaataatc
     7921 ttctcaactc tatcacctta gcaggaatat tcactcaatt ctatcatctg atgatgccct
     7981 gcattataca acctgtcaat cgagaaactt ctgaatctcc actctgtgac catcgggatc
     8041 tttgagaaaa aagtgatata ttccgaatct ctcgtttacg gaaggtttgt tcggcgacat
     8101 ttccagtctg agggcttttt cgtaaaaatc atctacgaac tccgtgagaa aagtcactat
     8161 tggcgagaac atacccgcct cgactggaag gtgttcacaa aagcctatca tacctccgga
     8221 tggtactgag taaattctac aacttccctg atccttgaac agctccagac ccagaaagtc
     8281 tctataaaat t
//
LOCUS       DAOOLY010000092         8249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10189_length_8249_cov_4.288992, whole genome shotgun sequence.
ACCESSION   DAOOLY010000092 DAOOLY010000000
VERSION     DAOOLY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10189_length_8249_cov_4.288992"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLD15_06075"
     CDS             <1..556
                     /locus_tag="PLD15_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB63506.1"
                     /translation="ALGSKIHDVKDPGKQLGNMFREVVGTLFALMEKYREKWEAIAGY
                     ETVPVYGEAVGGEAEPLQVDMDNMMSKCYEGIRTRLDFSESVLGGEITQKLSTFSKEN
                     AIVADDLWVEVVYKISSAYRKRELRSRLIELLVPVYFARVAGFVARTLEMNQEEAERE
                     TDRLLERFVDTKEELRKVWEKNTQ"
     gene            535..1356
                     /locus_tag="PLD15_06080"
     CDS             535..1356
                     /locus_tag="PLD15_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB63507.1"
                     /translation="MGEEYAINGKLKAMLKGLNPAEQKAARYIIDFPDDVIHHSISEL
                     SVLAGTSETTIFRLSKKLGFDGYQSLKIQIARELSLSTPVVYADKNELISELIGALNQ
                     QNQLLDDGQLDGISRRIVRANRLIFFGVASSGIVAEFGATLFMRAGFSTAYYTDPHLQ
                     IMTAVSLNEKDIVFGISASGNIRDTVKSIEVATDSGAYTIAITGGVGSRIVSAAKETI
                     YVAQGGHETGIPQLQPRVCQLVVIQLLLEKVIKMRDDTLKTLEKVDRTLDKKRYL"
     gene            complement(1668..2072)
                     /locus_tag="PLD15_06085"
     CDS             complement(1668..2072)
                     /locus_tag="PLD15_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63508.1"
                     /translation="MKRLSLLVVLAVAVIILLTGCPFIPKNISLAGTWNLYISVGGEV
                     VTDAAIMEITSHANGQLAGFVKANPGSGSIGSVAGTTSSDNITFIFTHISGERTVHFT
                     GKFSRNAMHGTAIAYDNGTYVSGPHNWTAQRQ"
     gene            2442..3440
                     /locus_tag="PLD15_06090"
     CDS             2442..3440
                     /locus_tag="PLD15_06090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPB63509.1"
                     /translation="MDYSKYTYEQLLKKVVELDMLNRELLREREQETRLDYSWAGNLG
                     HWYWNIKSNSVVFNPLKVTVLGYSMEELPREVNYQYFTERLHPDDYQGTMDAMLLHMH
                     GKKTVYEAEYRIQAKDGSWKWFYDRGKITQRDEKGAPLFATGIVFDITRNKERELNLE
                     RRSKDLERDARTDPLTGIGNRRAIIGELEDRLVEASINRTPISVIMLDIDHFKVLNDT
                     KGHLFGDSVLKEIATIIRNNLRGLDTVGRYGGEEFLVVLPNTAEENAASVAERIRRKV
                     EDFDFGEGSRVTISAGVGESPGGEDIETLIDLADKRLYEAKRTGRNVVIASRNGEP"
     gene            3578..4675
                     /locus_tag="PLD15_06095"
     CDS             3578..4675
                     /locus_tag="PLD15_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB63510.1"
                     /translation="MQQLFSNFAIPIGNLVMFAIGGILIYLAIKKDYEPMLLLPIGFG
                     AILTNIPGSSAIGEHGVLTILYEAGIANELFPALIFIGIGAMIDFTPLLQKPIMFFYG
                     AAAQLGIFLTIIVAAILGFDIREAISIGIIGTADGPTSIYVASRLAPQMLGPISVAAY
                     SYMALVPVVQRPIIKLMTTKDERRIRMDNRTEKVPKYVKILFPIFVTILAGIVAPVSV
                     ALIGMLMFGNLIRECGVLNKLSNAAQNELSNLITLFLGITIGSTMTAQAFLNTRTLLI
                     LVMGLVAFILDTMGGIAFAKIFNLFRKQKINPMIGAAGISAFPMSARVVHKLGLEEDP
                     SNFLIMYATGANVAGQIGSVLAGSIVLALFI"
     gene            4700..4834
                     /locus_tag="PLD15_06100"
     CDS             4700..4834
                     /locus_tag="PLD15_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63511.1"
                     /translation="MSEAVSKSLAIFAEGYPTTFLITVAFFIMIKLLVWTSSRKKKTD
                     "
     gene            5009..5911
                     /locus_tag="PLD15_06105"
     CDS             5009..5911
                     /locus_tag="PLD15_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPB63512.1"
                     /translation="MGKILSENGKALFAYVLVCFFWGSTYLAIKIGVQDMPPMFFASV
                     RFIVAGGLMLLFSKVRRLTFPSSFKEVALLSTVGLFMLLGGNGLVVFAEQWVDSGVAS
                     LMIATVPIFVAILEHFFIKGTKLTLKGVFGLLLGFLGVYFLLHPSGDSSSINLEGVAV
                     LLTASFLWSSGTVLSKKVRSNGSIVASIGIQMFAGGIGLMITSLISGELPRVRFTANS
                     LYAILYLIVFGSLIGYSSYIYLLQKWPATRAGTYAYVNPAVAVTLGALLLGERVTVLM
                     LISMVAILVGVIIVQRSKIRVDQK"
     gene            6011..6328
                     /locus_tag="PLD15_06110"
     CDS             6011..6328
                     /locus_tag="PLD15_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63513.1"
                     /translation="MCKGLFVVDLTMVVFDLGVIMQCERKTEVVRNLAGAIIESYNQS
                     STSERMHLSEVEEFLKLLELSLFIQCYDFYTRSSPLQGWLKLFYEGREERILSDAPFL
                     AWK"
     gene            6541..7488
                     /locus_tag="PLD15_06115"
     CDS             6541..7488
                     /locus_tag="PLD15_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63514.1"
                     /translation="MRFAGFAILFFVTFLLFVITLLSSFGYEPIMVPLLVLVITGFVI
                     ESVRALLSGKTTPTDRSHFLKTMVAVMAGAIGAYIMNCSLSISAIMAASLVGVIAVAV
                     TKKFAVEVYCGAFVGMVSPQVLHDFTHTIFAGLIAGIVFYLARDVFNGYGGKLGTIAC
                     SSWIIMTAISSVKLLDTQVEIRHSGLSIMFYSLGSALLTYMLSIRFKNGPVLASSVVS
                     LAGAVILPAVHGVSSGLLTGVVMAASFAGMSSKEKMRNELEILLSGALLGIMFIYSAN
                     HFNGAGGRLGTLAFGSVVSARGIIFLIQKAARRRPLLSN"
     gene            complement(7497..>8249)
                     /locus_tag="PLD15_06120"
     CDS             complement(7497..>8249)
                     /locus_tag="PLD15_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPB63515.1"
                     /translation="KSSLVDTLRSGGPYTLFAPSDIAFARVPAETREALLNEPQWLKA
                     VLLYHVVQGEYTTADLLNEKGLRSLHGELLNVKINEAGLGVDNTLITIGDIDTGNGVI
                     HVIDHVMMPREWRSGLPSIVEAAEQAGQFTTLLAALKATGLEETLSDGGLLTVFAPTD
                     EAFAKLGQETIDGLLKDTEKLKSILTYHVIGGEYLFSELKIAQSLFTVNGKPVKINLD
                     GTLTINNVNIISRDILTRNGVIHVIDAVLIPR"
BASE COUNT         2003 a         2009 c         2237 g         2000 t
ORIGIN      
        1 cgctctcggg agcaagatcc acgacgtaaa agacccgggc aaacagctgg gaaacatgtt
       61 cagggaagtc gtcggcactc tgttcgcact gatggaaaaa taccgcgaga agtgggaagc
      121 gatcgcagga tacgaaacgg tacccgttta cggagaggcc gttggaggag aggccgaacc
      181 tttacaggtg gatatggaca acatgatgag caaatgttac gagggtatcc ggacccggct
      241 ggacttcagc gagagtgttc tcgggggcga aatcacacag aaactctcga ccttctcgaa
      301 ggaaaatgcg atagtggccg atgatctgtg ggtcgaggtc gtatacaaaa tctcatcggc
      361 ttacaggaag agagaactca gaagcagact gatagaactt ctggtgccgg tgtacttcgc
      421 aagagtggcc ggcttcgtcg ccagaacgct ggagatgaac caggaagagg ccgaacgcga
      481 aaccgacaga ttgctcgaaa gattcgtcga caccaaagaa gagttgagga aggtatggga
      541 gaagaatacg caataaacgg aaaattgaaa gcgatgctca aaggtcttaa tccggccgaa
      601 cagaaggccg ccagatatat tatagacttc ccggacgacg tgattcacca ctccatcagc
      661 gagctgtccg ttctggccgg aactagcgag accacgattt tcagactttc gaaaaaactg
      721 ggcttcgacg gttaccagtc ccttaagata cagatcgcca gagagctttc tctctccacc
      781 cctgtcgtgt acgccgataa aaacgagttg atctcggaat tgatcggtgc gctcaatcag
      841 cagaaccagc tgctggatga tgggcagctc gatggaatat ccaggcggat cgtaagagcg
      901 aacagactga tatttttcgg agttgcctcg agcggaatcg tggccgagtt cggagccacg
      961 ctcttcatga gggccggttt ctcgacggct tactacaccg atccgcatct ccagatcatg
     1021 acggccgttt cgcttaacga gaaagatata gtcttcggta tctccgccag tggcaatata
     1081 cgtgacacgg tgaaatctat cgaggttgcc accgattccg gcgcctacac catagcgata
     1141 actggagggg tcggctcgag gatagtgagc gcggcgaaag agaccatcta cgtcgcccag
     1201 ggtggtcatg agacaggcat accgcagctg caaccaagag tctgccagct ggtcgtgatt
     1261 cagttgttgc tggaaaaagt gatcaaaatg agggacgaca cgctgaaaac tctggaaaaa
     1321 gtcgacagga cactggacaa gaagagatat ctgtagtctc gactcgcacg tttccgatca
     1381 acgaaaagcc gtagcccata tctttgagac gtccagcgtt gtcgtccaag agcgtggacc
     1441 cgtccttggg gaacctgagg cgagaggctg gaagagcgat aagatctcga ctttcttacc
     1501 tctggcctca tccctcttgc ctctgtcgtc tttgcatgcg ttccgccact gcacgtccag
     1561 gttcttgcgc tgtttacccc gatccatagc gcggccggcc gttttaagct ccgcctcgtt
     1621 tcttaactcg aagtgcgtcg agttttatag tgtattaggt tgaatttcta ttgcctttgc
     1681 gccgtccagt tatggggtcc cgaaacgtaa gtgccgttgt cgtaagcgat agccgttccg
     1741 tgcatagcgt ttctgctgaa cttgccggtg aaatgaacgg ttctttctcc cgagatatgc
     1801 gtgaaaatga atgttatatt gtcggaggaa gtagttccgg ctaccgaccc gatgcttcca
     1861 gacccgggat tagctttcac aaatccggcg agctgaccat tggcatgact ggttatctcc
     1921 atgatggccg catcggtaac cacttcacca ccgaccgaga tatagaggtt ccatgtaccg
     1981 gcaaggctta tgtttttagg aatgaacgga caccccgtta gcaagataat aacggcaact
     2041 gcgagaacaa ccaaaagaga taaacgtttc atgatcgaca tccctccctt atagttatag
     2101 gaccaatcta cgatacatgt ttgtattcgt tcagcgtaag ggaaaattac atcgagtctg
     2161 cttcggccac aggataggcc tcttaaaact acatctcgac aaataatata ggcaggaagc
     2221 atcaaagata tcattactat agcacttacc gacccagttt caatgatgga tggacagttc
     2281 gattttcacc gatacaccag gctgtctgga gttgaatgac cggactgtga taccggatat
     2341 gatagaattc tgtgaacgct cccctcttcc ccggccgcat ttggatagat caatccgccg
     2401 gtttcgggga gctgcgaact ccagaccaag gggtgaaaag catggattat tcaaaataca
     2461 cttacgaaca gcttctcaag aaggttgtcg agctggatat gctcaacagg gagctgctca
     2521 gggaaaggga acaggagacg aggctagatt attcgtgggc cggaaacctc ggtcactggt
     2581 actggaatat caaaagcaac tccgtagtct tcaatccatt gaaagttacc gttctgggct
     2641 acagtatgga ggagttgccg cgggaagtca attaccagta tttcacagaa aggctccatc
     2701 ccgacgatta tcaaggcacg atggacgcca tgcttctcca catgcatgga aagaagacag
     2761 tttacgaagc cgaatacaga attcaggcta aagacgggag ctggaagtgg ttttatgaca
     2821 ggggaaagat cacacagaga gatgagaaag gcgcgccgct tttcgctacc ggcatagtct
     2881 tcgatataac ccgcaacaaa gaaagagagc tgaaccttga gagaaggagc aaggatctcg
     2941 agcgggatgc ccggacagat ccattgacag gcatagggaa caggagggcc ataatcggcg
     3001 aactggaaga tcgattagta gaggcttcta taaacaggac ccctatttcg gtgataatgc
     3061 tcgatatcga tcatttcaag gttttgaacg atacgaaagg ccatctgttc ggagacagcg
     3121 ttctgaaaga aatcgcgacc attattcgaa acaacctccg tggactcgac acggtcggaa
     3181 ggtacggggg agaagagttc ctcgtcgtac ttccaaacac cgccgaagaa aatgctgcat
     3241 ctgttgccga aaggatcagg aggaaagtcg aggacttcga cttcggcgaa ggttccagag
     3301 tgaccatcag cgcaggggtg ggagaatcac cgggaggtga ggatatcgag acgctcatag
     3361 acctggccga taagagattg tacgaggcta aaaggaccgg gaggaacgtc gtgatcgctt
     3421 caagaaacgg agaaccctga agttatctgg atctacaaaa aaacccattg cagcgctgaa
     3481 atagcgcaga aaatgagata atattgtact gaaaatcggc gaggttcaaa agcagaatta
     3541 gaacgctaac tgaagatagg acaaaggggg aagttctgtg cagcagttat tttcgaactt
     3601 tgcgataccc ataggcaatc tggtcatgtt cgccatcggg ggaatcctga tctacctggc
     3661 cataaagaaa gattacgaac ctatgcttct gctgccgatc ggattcggcg ccatcctcac
     3721 gaatattccc ggttcttcag ccatcggaga gcacggggtg ctgacgattc tctacgaggc
     3781 gggaatcgcg aacgagctct tccccgcgct gatattcatc ggcatcggcg ccatgataga
     3841 cttcaccccg cttcttcaga aaccgatcat gttcttctac ggagcggcgg cccagctggg
     3901 tattttcctg acgataatag tggccgcgat ccttggattc gatataagag aggccatctc
     3961 gataggcata atcggaacgg ccgacggccc gacatccata tacgtcgcca gcagactggc
     4021 cccgcaaatg ctcggtccca tctcggtggc cgcttattcc tacatggcgc tagtgcccgt
     4081 agtgcagagg ccgataatca aattaatgac caccaaagac gagagaagga tcaggatgga
     4141 caacaggacc gagaaagtgc ccaaatatgt gaagatactc ttccccatct tcgtcacgat
     4201 tctggccggc atcgtcgccc cggtgagtgt cgcgctgatc ggaatgctga tgttcggaaa
     4261 tctgatccgc gagtgcggag tcctgaacaa gctgagcaat gcggctcaaa acgagctttc
     4321 caaccttata actctctttc tgggcatcac gataggctcc acgatgacgg cacaggcctt
     4381 ccttaacacc agaacgctcc tgatcctggt gatgggactg gtcgccttca ttctcgacac
     4441 tatgggcgga atagccttcg ccaagatctt caacctcttc cgcaaacaga agatcaaccc
     4501 catgatcggg gcggccggaa tctcggcctt ccccatgtcg gcgcgcgtcg ttcacaaact
     4561 cggcctggaa gaagacccga gcaacttcct catcatgtac gccaccggag ccaacgttgc
     4621 agggcagata ggttcggtcc tcgccggaag catcgttctg gctctcttca tatgagcacg
     4681 atcggaagga gggatctgta tgagcgaagc ggtatctaag agtctggcga tattcgccga
     4741 ggggtatccg acgacttttc tcatcactgt cgcgtttttc ataatgataa agttgcttgt
     4801 ctggacttct tcaaggaaaa agaaaaccga ttgaaagagc aaaagcctat tggtaatcat
     4861 gggctttccc gggtcggatc tatcgccgtt gatctatctg tcacacaatg tttcgtgaag
     4921 cgaacgaaca tctcccgtct cgtttgtaca taattcatca ataagccgcg tccgcatacg
     4981 gacagtttgt tttaccgggg ggtagacgat gggaaagatc ctgagcgaaa atgggaaggc
     5041 cttgttcgcg tacgttctcg tctgtttctt ctggggctcg acatacctgg cgataaagat
     5101 cggcgttcag gatatgcccc caatgttctt cgccagcgtc aggttcatcg ttgccggggg
     5161 tctgatgctc ctcttctcaa aggtcaggag attgactttc ccctcgagct tcaaagaagt
     5221 agcactgctt tcgacggtcg gtctcttcat gctccttggc ggcaacggtc tggtagtctt
     5281 cgccgaacag tgggtcgatt cgggcgtggc ctcgttgatg atcgcaaccg ttccgatatt
     5341 cgtggcgatt ctcgaacatt tcttcataaa ggggacgaag ctgactctca agggtgtttt
     5401 cggtctcctg ctgggctttc tcggagttta cttcctgttg catccatctg gagattcgag
     5461 ttcaataaac ctcgaaggcg ttgcggtgct tctgacagcg agtttcctct ggtcatcggg
     5521 aacggtgctt tcaaagaaag tcaggagcaa cggctcgatc gtggccagca tcggaattca
     5581 gatgttcgcg ggtggaatcg gactgatgat cacctccctg atctccggag agctgccgag
     5641 ggttaggttc accgccaact cattgtatgc gattttgtat ctcatagtct tcggttcgtt
     5701 gataggctac agcagctaca tctacctgct tcaaaaatgg cccgcgacca gggccggcac
     5761 ttacgcctat gtcaatcccg cagtcgcggt aacgctcggg gcgctcttgc tcggggagcg
     5821 tgtgacggtt ctgatgttga tctctatggt ggccatactg gtgggagtca tcatcgtaca
     5881 gagatcgaaa atcagggtcg atcaaaagta gaagcctcgc cagcagccct actttatcga
     5941 ctcataaaag taggggagaa tagcgagagc agggattctg gaaatcgcgt ctctgtacga
     6001 tttcgacgat atgtgcaaag ggctcttcgt tgtggacctg acgatggtgg tgttcgatct
     6061 cggagtgatc atgcaatgtg agcgaaaaac cgaagtcgtg agaaatctgg ccggggcaat
     6121 aatcgaaagt tacaatcagt ccagcacaag tgagagaatg catctgtctg aagtcgagga
     6181 gtttctgaag cttctcgaac tgagtctctt cattcagtgt tacgatttct atacccgatc
     6241 gagtcctctg caaggctggc tgaagctctt ctacgagggg cgggaagaga gaatactttc
     6301 cgacgcgcct tttctagcct ggaagtagtc ctgaagaaga gactcggcga cacgctttac
     6361 ttcgattgat ctgtcctcat acctcttatc tagccacatc gcgcacgcac atgcgatcat
     6421 tgcgaacgat tactgtataa ttgaattgtg attcttctat ttgtaccgga accaaatgta
     6481 ttaaaatccg atcgtggccc gttgtagatt tctttgccac gtagttttcc gggggttcgt
     6541 atgaggtttg caggattcgc catattgttc tttgtgacct tcctgctttt tgtaattacg
     6601 cttctttctt cttttggtta tgagcctatc atggtgcctc tcctcgttct tgtgattacc
     6661 ggatttgtga tcgaaagtgt gagagccttg ctttcgggaa aaaccactcc cacggatcgc
     6721 tcgcattttc tgaagactat ggtcgcggtt atggccggag ccataggcgc ctatatcatg
     6781 aactgctctc tctctatcag cgcgattatg gcggcgagtc tggtgggggt gatcgcggtc
     6841 gcggtcacga agaagttcgc ggttgaggtt tattgcggtg cttttgtcgg aatggtttcg
     6901 ccccaggttc tgcacgattt cacccacacg atattcgccg ggcttatcgc cggtatagtc
     6961 ttctacctgg ccagagatgt tttcaacggt tacggaggaa agctcggaac gatagcctgc
     7021 agcagctgga tcataatgac ggccatttca agcgttaagt tgctggatac tcaggtggag
     7081 atccgacact ccgggttgag tattatgttt tacagtctgg gatcggccct gttgacgtat
     7141 atgctcagta ttcgctttaa aaatggtccc gtgctggctt ccagcgttgt gagtcttgcc
     7201 ggcgcggtga ttttgccggc cgttcacggc gtgagctccg gtttgctgac cggggtggtg
     7261 atggcggcat cctttgccgg aatgtcttcc aaggagaaga tgaggaacga actggagatc
     7321 ctgctgagcg gagctctgct gggaataatg tttatctata gcgcgaacca tttcaacggc
     7381 gccggaggaa gactcgggac gctggccttc ggaagcgtcg tcagtgccag aggaattatc
     7441 tttctaatac agaaggccgc ccgaaggcgg cctctgttga gcaattaggg ttaagcttat
     7501 ctgggtatca ggacggcgtc tatcacgtgt atgacgccgt ttctggtcag gatgtctctg
     7561 ctgattatat taacgttgtt tatggtcagc gtaccgtcca ggtttatctt taccggttta
     7621 ccattgaccg tgaagagcga ttgcgctatc ttcagctcgg agaataagta ttctccgcct
     7681 atcacgtggt aggtgaggat cgacttcagc ttttcggtgt ctttcaggag tccgtcgatg
     7741 gtttcctgcc cgagtttggc gaaggcttcg tcggtcgggg cgaagaccgt caagaggcct
     7801 ccgtcagaaa gcgtctcttc tagaccggta gccttcaaag ctgcgagaag ggttgtgaac
     7861 tgtccggcct gttctgcggc ctcaacgatc gaaggcaggc cgcttctcca ttctcgcggc
     7921 atcatcacgt ggtctatcac atggatcaca ccgtttcccg tgtcgatatc gccgatcgtg
     7981 ataagggtgt tgtcaacgcc aagcccggct tcattgattt tcacgttcaa cagttctccg
     8041 tgaagcgagc gcaatccctt ttcgttgagc aggtcggccg ttgtgtactc tccctgcaca
     8101 acgtggtaga gaagcactgc cttgagccat tgtggttcgt ttagaagcgc ttctctggtc
     8161 tcggcaggaa ctctggcgaa ggcgatatcg ctcggggcga agagagtgta cgggccgcca
     8221 cttctgagcg tatccacaag gctggattt
//
LOCUS       DAOOLY010000093         8235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10210_length_8235_cov_4.186675, whole genome shotgun sequence.
ACCESSION   DAOOLY010000093 DAOOLY010000000
VERSION     DAOOLY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10210_length_8235_cov_4.186675"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLD15_06125"
     CDS             complement(<1..436)
                     /locus_tag="PLD15_06125"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HPB63516.1"
                     /translation="MLGYFRPGTSLSAESKRQYRIIYCSLCHSLRNTYGRRAALLLQN
                     DSLFFALQSGFMPPVDEEKVVKKRCVMQPKKVEILVSSPEVFEPLADFSLLTVLFAGW
                     DNELDERRGVKSRLFDTLYRRVFTKLGAKLRQRGVSLDGLKRE"
     gene            604..1362
                     /locus_tag="PLD15_06130"
     CDS             604..1362
                     /locus_tag="PLD15_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63517.1"
                     /translation="MKVCYYGDQCPWNSWARGEARKAADALGLSIEEYNLTHLSPPVG
                     LFFPFSIFLKDITIAGPVPARRLIDILKTGKPSEYFEPYSPNPKGSCDSIRLYSRETI
                     TDACRICTNGDNFGIAKKRAWLERFDSKDRLCAGAVAYSGERPVAFCEAIPSLESPYA
                     IPAEGSMAFINCIYNAMAEPLDYRAGLLEFFLGRLRALGYSKVGVLSGLRAPFPNGPE
                     SFFLGMGFTRTRQIPGTILMNSGLDEIVFLSREL"
     gene            complement(1470..2939)
                     /locus_tag="PLD15_06135"
     CDS             complement(1470..2939)
                     /locus_tag="PLD15_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPB63518.1"
                     /translation="MPSHNSLWDTWLFEENGLYHLFHLLRKKGSRSHAINHAVSEDLL
                     HWKEAGLALERGYKGEWDDGPLMTGTLCKNNGIYYMFYGAKREAIQRIGIAISEDLLT
                     WRKIPRHVLEPQGEWYETSVVDSSISSVAWRDPCIVRTDEGDFHMFFCARSRTGNWAG
                     RGAIGHAISRDLIEWNILPPIYVSDKYGFLETPDVFEINGRWFLIFSCGQWHSTRTSD
                     FEYAASGIRYLVGESLTGPFHEPSESSLLGSGDGMLGSYVGRTLPLRGEHESRLFYHH
                     NVFPEKITNFKSLTGSFSSPKILKLCGDYLSLQKYDDFDRKIFPNSSRLKREHLAARY
                     PESEDTGVQWIPRDMSIEALSSAGIAGVWFDKPIEDSAIRVDIRLKRGNGGILFGFEE
                     YNKGFGIILNYYKQRVELQHVFRAEFGLSTSMIQYRPLRLSFFKEYRIELIIVSPFVE
                     LYVDNFLILSQPVEGSIAGRLGLLIEGGLGIFSYPDILY"
     gene            complement(3129..3971)
                     /locus_tag="PLD15_06140"
     CDS             complement(3129..3971)
                     /locus_tag="PLD15_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63519.1"
                     /translation="MYLSKKKRIRRTLLVHAALFILTFIILLPVLWMILTSLKPTAET
                     LKFPSTILPDNPTIQNYLAAWRNMRLGTYFINSLYVSLMTTFVAIVLGCMGGYALARF
                     RMKGEKAYVMFILVTRMFPAVVFLIPFFIMMRNLQLTDSFTGLIISYTVFSLPFSIWL
                     LRGFFRNIPSELEEAARIDGCTRFKAFTRIILPLSAPGIAATAVFCFILAWNEFMFAN
                     TLIMSDSKRMLTVALVASISDFLVKWNELMAGGMITTIPVIIFYLFMQKYITGGLTEG
                     SVKG"
     gene            complement(3984..4829)
                     /locus_tag="PLD15_06145"
     CDS             complement(3984..4829)
                     /locus_tag="PLD15_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63520.1"
                     /translation="MSPTIAVMVLIIIVPLIIGLVMSFFNYNLLTLHRGIPFIGFSNY
                     SKALGDENFWMAFWNTLWWTAANVAGQVALGLSIALLLNQPVKGVGIYKALILIPWAV
                     PSAVAALSWMWLLHPEFGLVNDIAVRFGLVSEPVAWLGRPFTAQVWVVIARVWKEFPL
                     SALIFHAALQTIPKDLYEAAEVDGAGVWKRFWNITIPYLKASIVVAITLITIWTFNSF
                     NMIWVMTKGGPGGATEILATSIYKTAFQRYNFGFASSQSILMVIFLSVIIIFYLYKVE
                     GKKID"
     gene            complement(4974..6215)
                     /locus_tag="PLD15_06150"
     CDS             complement(4974..6215)
                     /locus_tag="PLD15_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB63521.1"
                     /translation="MTVKKILLIAAVMLVFFAFVGGKTVITMWVYDEMAANEEMELVK
                     AQKEFMERNPTIELKIENVPNRGLMEKFITASLAGVAPDIIHVSTTWAVELGAIGTLL
                     PLNDYISASGKDKEILKSAIDSSTLDGHIYALPWISDATALIINKTAFEKAGINYSRD
                     TAMNWSEFLEVAKKLTFDNQYGFGIRSGRGASYGWFPWLVANGGSLFSPDGTKVTFNE
                     PEGKEAFKFYAELFTTHKVIPPGAIAYDTWDDIRNSFLGGRIAMYIAGDWEIAPLRAA
                     NAAFEIDVIPHPMQVRRAASLGGSSLGIWSGSKAKDAAWAWLDFLTSAENMKVAFSYG
                     RFPSRVDALDSGFINDPLIEQFIREFPYGINHASLVGDYSRTVLGDAFTSVLVYGENP
                     DIALDKAAKTIQEALNEVLGK"
     gene            complement(6262..7029)
                     /locus_tag="PLD15_06155"
     CDS             complement(6262..7029)
                     /locus_tag="PLD15_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPB63522.1"
                     /translation="MLSEERRRQILRELSIKGKVRSTEIASELGVSEVTIRSDLSELE
                     QEGLLKRVHGGAIQTKLQRYDPEFQEKVAIDVDEKRHIALKATKFVEKYDVIFIDAGS
                     TTLFFFEELSKNPPTILTVVTNSLYIINAVVQYPNINLVVIGGTFQHNTMNFLDFEIN
                     PFLNRYNVNKVFLGVNGFDETGYYASNILEAETKKLICNACDEIFILATSSKHFKRSL
                     VLTQKWTGKERIITSDLTEDNRNRLMEVARNNNLSIY"
     gene            complement(7528..>8235)
                     /locus_tag="PLD15_06160"
     CDS             complement(7528..>8235)
                     /locus_tag="PLD15_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPB63523.1"
                     /translation="ILKTAINSVSVSDSDWQMMRNSNRSPAFEQIDSVAVLKIPSFHP
                     DYQEETLELMYRIPGDGYSDLLIDVRDNCGGFQLFLEAVLGYLTKERADLVEKIGTRD
                     SSGQYTIRTWNLPIYPDYSWKAKDSRIWILTNANVNSATLLFLSFMRRNTDAVVIGER
                     SSEPVNFGITNPFHTMPNTGLLFCLSKILVINGAEGEHIEPDLVIEQSLEEEINWYLG
                     NGDATLQKAIDTITGRN"
BASE COUNT         2228 a         1992 c         1806 g         2209 t
ORIGIN      
        1 attccctctt caacccgtcg agcgatactc ccctctgtcg gagtttcgcc ccgagttttg
       61 tgaaaactcg cctgtaaagc gtgtcgaaca acctcgattt cactccccgc ctctcatcca
      121 gttcgttgtc ccatcctgcg aagagaacag ttaaaagcga gaagtcggcc agaggttcga
      181 aaacttccgg gctggagacc aatatctcga cctttttggg ttgcatcaca catctttttt
      241 tcacgacttt ctcctcatcc acaggaggca tgaagcctga ttgaagcgcg aaaaagagcg
      301 agtcgttctg aaggagaagt gcggccctcc tgccgtaggt gtttcgtaga gaatggcata
      361 gcgagcagta tataatccgg tactgtctct ttgactctgc ggaaagcgag gtacctggcc
      421 tgaaataacc caacatcttg tctccttcgg cagctggatc ggttgttgag attatatcgg
      481 aaggtgcata accagtcaat tgcacaacta gaacgaaggc cggttgatac tcgcccggaa
      541 aacctataat attggtacca gatcgacata aattacacgg gcggggtgaa agagatcgaa
      601 agaatgaagg tttgctacta cggcgatcag tgtccctgga attcatgggc gcgtggagag
      661 gcaaggaagg ccgccgatgc tctggggtta tcaattgaag agtacaacct cacacatctg
      721 tctcctcccg tcgggctctt ctttccattc tcgatcttcc tgaaagatat cacaatagcc
      781 ggtccagttc cggcccgacg actgatagac atattgaaga ccggaaaacc atctgaatac
      841 ttcgagccgt attcgccgaa cccgaaggga tcgtgcgact ccataaggct ctattcgagg
      901 gagacgatca ccgatgcgtg ccgtatatgc actaacggcg ataacttcgg catagccaaa
      961 aagagggcct ggttggagag gttcgacagc aaagatcgcc tctgcgccgg agccgtggcc
     1021 tactcgggtg agagacccgt ggccttctgc gaggctatcc cctctctcga gtcaccttac
     1081 gccatcccgg ccgaaggttc gatggccttt ataaactgta tctacaacgc catggctgaa
     1141 cctctagact acagagccgg attgcttgaa ttctttcttg gtcggctgag agctctggga
     1201 tattcaaagg tcggtgttct ctcggggctt cgcgctccct ttccgaatgg tcccgaatcc
     1261 ttctttcttg ggatgggatt cacccggacg cgacaaatac ccggtacgat actcatgaat
     1321 tccggcctcg acgagatagt ctttctatcc agagagctct gaggaccggg ctaaaatggt
     1381 ttttcatatg gcaaaatctg caagcgcttc tattttagaa gagcattctg gaagtgccat
     1441 ctttcttgca ccctcaacga agatcgctat tagtaaagga tatctgggta tgagaaaatt
     1501 cctaatcctc cctcaattag aagtcccagt cttcctgcaa tactaccttc aacaggctga
     1561 ctgaggatca gaaagttatc aacatatagt tcaacaaaag gagagactat tatcagttca
     1621 attctatact ctttgaaaaa cgagagtctt aacggtctgt actgaatcat actggttgaa
     1681 agcccaaatt cagcacggaa aacatgttga agctcgacac gctgtttgta gtaattgagt
     1741 atgattccaa accctttgtt gtattcttca aatccaaaaa gaattccacc attgcctctc
     1801 ttaagcctta tatcaactcg gatagcgcta tcttcgatag gtttgtcaaa ccatactcca
     1861 gcgattccag ctgacgagag tgcctcgatt gacatatcgc gggggatcca ctggaccccc
     1921 gtgtcttcac tttcaggata tcgtgctgcc aggtgctcgc gtttcagtct ggaggaattc
     1981 ggaaagatct ttctgtcgaa atcatcatac ttttgcaggc taaggtaatc accgcagagc
     2041 ttaaggatct tgggagaaga gaaagagccg gtcaaactct tgaaattcgt gattttctcg
     2101 gggaagacat tgtgatgata gaagaggcgg gattcatgtt cgccccggag tggcagagtt
     2161 cttcccacgt aggaaccgag cattccgtct ccagaaccca acaacgaact ctccgaaggt
     2221 tcatggaagg gtcctgtgag agactcgcca accaggtatc taattccgct cgccgcgtat
     2281 tcgaagtcgc tggtgcgagt gctgtgccac tgcccacatg agaagatcag gaaccatctg
     2341 ccattaatct cgaaaacatc tggcgtctca agaaacccgt atttatcaga tacataaatg
     2401 ggtggcagta tattccattc aatcagatct cttgatatgg cgtgacctat agctcccctc
     2461 ccggcccagt tgccggttct tgagcgggca cagaaaaaca tgtggaagtc gccttcgtcg
     2521 gttctgacta tgcaggggtc tctccaggca acgctcgaaa tcgagctatc gacaaccgag
     2581 gtttcatacc attcaccttg aggttccagt acgtgacggg gaatttttct ccacgtcaac
     2641 aggtcctcag agatcgcgat tcctattctc tgaatggcct ctcgtttggc tccatagaac
     2701 atatagtaga tgccgttgtt cttgcagaga gttccggtca tcagaggacc gtcgtcccat
     2761 tctcctttat agcctctttc aagtgccagc ccggcttctt tccagtgcag cagatcttcg
     2821 gacactgcat gatttatggc gtggcttctt gaacccttct ttctgagaag atgaaagaga
     2881 tgatacagac cgttttcttc aaagagccag gtgtcccaca gactgttatg agacggcaca
     2941 aacagcactt caaccactcc tgtcagttca acagttgtct cttccagacc ggaacactag
     3001 tctggtaccc accaaaacga taggattatc cagataattt aacctccaga gattattgat
     3061 gaagattcca accatcgaga gaaaatataa cctactgaag tagagtcaca gaaacgaaga
     3121 gcaagcgctc aacctttcac gcttccttca gtaagaccac ctgtaatgta tttctgcatg
     3181 aacagataaa agattatcac cggaatggtt gttatcatac cgcctgccat tagctcattc
     3241 cacttcacaa gaaaatcgct tatagaagca acaagggcaa ctgtcagcat tctcttgcta
     3301 tctgacatta tcagagtatt tgcgaacatg aattcgttcc atgcaagtat gaaacagaag
     3361 accgccgtcg ccgcgatacc cggggcggat agaggcagaa ttatccttgt aaaggcttta
     3421 aaccttgtac aaccatcaat tcttgcagcc tcttcaagct cgctcgggat atttctgaag
     3481 aatcctctca ataaccatat tgaaaatgga agcgaaaaga ccgtatacga aattatcaaa
     3541 ccggtaaaag agtctgtcaa ctgaaggttt cgcatcataa tgaagaaggg gataagaaat
     3601 accaccgctg gaaacatacg ggtgacaagt atgaacatta catacgcctt ttctcccttc
     3661 atcctgaatc tggcaagtgc gtaaccgccc atgcacccaa gaacaattgc aacaaaggtt
     3721 gtcataagtg atacatacaa actattgatg aagtatgtac ccaatcgcat gtttctccag
     3781 gcggcaagat agttttgaat cgttggatta tcaggaagta tggtcgatgg aaacttcaga
     3841 gtctccgctg tgggctttag agaagtgaga atcatccaga gaactggtaa tagtattatg
     3901 aatgtgagaa taaagagagc agcatgaact aacagcgtcc ttcttatcct cttttttttg
     3961 ctcaaataca tgatcactca cctctaatct attttcttgc cttctacttt gtaaagatag
     4021 aagatgatta tcaccgacag gaatattacc atcagtatcg actgtgaaga cgcgaatcca
     4081 aagttgtatc gctggaaggc tgtcttgtat atactggtgg ccagaatctc agttgctcct
     4141 ccgggtccac ctttagtcat tacccaaatc atgttgaagc tgttgaaggt ccaaatagtt
     4201 atcaacgtta tcgcaactac gatggatgct ttcagataag gaatggtaat gttccagaat
     4261 ctcttccata ctcctgcgcc atcgacttcg gcagcttcat acagatcttt cggtattgtc
     4321 tgcagagcag cgtggaaaat aagagcactg agaggaaact ccttccagac tcgtgctatg
     4381 acgacccata cttgagcggt gaaaggtctc ccaagccagg cgaccggttc gctgacgagt
     4441 ccgaacctaa cggcaatatc atttaccaga ccgaattccg gatgcaacaa ccacatccag
     4501 ctcagcgcag caacggccga cggaacggcc cagggtataa gaatgagagc tttatagatt
     4561 ccgacaccct tgaccggttg atttagtagc aatgctatcg aaagaccaag tgcgacctga
     4621 ccggctacat tcgcggcagt ccaccaaaga gtgttccaga aagccatcca aaaattctca
     4681 tcgccaaggg cttttgaata attgctaaaa ccgatgaagg gtatccctct atgaagagta
     4741 aggaggttat agttgaagaa actcattaca agtccaataa tcaggggaac tatgattatc
     4801 aaaaccataa ccgctatagt cggactcatt agaaagaaac ccatatgctt atcgaaaaat
     4861 cgttttgtgt tcatccatta atcacgcacc ctgacataag attgtgatcg tcattaagaa
     4921 atcaggcaac ggagactaaa gactggtctc cgtcgccttt gtgcagattc tctttattta
     4981 ccgaggactt cgttaagggc ttcctggata gttttagcag ctttgtccag agcaatatcg
     5041 ggattttctc catagaccag taccgaagtg aatgcgtctc cgagaactgt tctcgaataa
     5101 tctccaacaa gagaggcatg gtttatgcca taagggaact ctcttataaa ctgctctata
     5161 agaggatcat taataaagcc actgtcaagc gcatctactc ttgaagggaa tcttccatag
     5221 gagaatgcca ctttcatatt ctctgcggag gtaagaaagt caagccatgc ccacgccgca
     5281 tcttttgctt ttgagccgct ccagataccc agactgctgc cacccagcga tgctgctctc
     5341 ctaacctgca taggatgggg aataacatct atttcgaaag cggcatttgc agcccttagg
     5401 ggagcaattt cccaatcgcc tgctatgtac attgcaattc gaccacccag gaaagagttt
     5461 cttatatcgt cccaggtgtc gtaggctatg gctcctggag gaatgacttt atgggtagta
     5521 aagagctcag catagaattt gaaggcttct ttaccttccg gttcgttgaa ggttaccttt
     5581 gttccgtccg ggctgaagag gctgccacca ttcgctacca accacgggaa ccaaccatat
     5641 gaggctccgc gaccacttct gattccaaaa ccgtattgat tgtcaaaagt caatttcttg
     5701 gcaacttcca aaaactcact ccagttcata gcagtatctc ttgaatagtt aatgcccgct
     5761 ttttcaaaag ccgtcttgtt tatgatcagt gctgtcgcat ccgatatcca gggaagagca
     5821 tagatatgcc cgtcgagagt gctggagtcg attgcacttt tgaggatctc tttgtccttt
     5881 ccacttgcag agatatagtc attcagtggc agcagtgttc caatagcgcc taattcgact
     5941 gcccaggttg tcgatacatg tatgatatcc ggggcaacac cagctagact ggcggtaatg
     6001 aacttctcca tcaatccacg gttaggaaca ttctcaatct tcagttcgat agttggatta
     6061 cgttccataa actctttctg ggccttgaca agctccattt cttcattagc tgccatctcg
     6121 tcatataccc acatggttat aactgtctta ccacctacaa atgcaaagaa tacaagcata
     6181 acagcggcga ttagaagaat tttcttaaca gtcatacaat ttccctcctc cgtaaggaat
     6241 gttgtcgtgc tggaagtctt ttcagtaaat cgatagatta ttgttcctgg caacttccat
     6301 gagcctgttt ctgttatctt ccgtcaagtc cgaggtaata atccgctctt tgcctgtcca
     6361 tttctgtgtc aacacgagcg accttttgaa gtgttttgaa ctggttgcca ggataaatat
     6421 ctcgtcacac gcattgcata tcagtttctt tgtttctgcc tcaagaatgt tcgatgcata
     6481 gtagccagtt tcgtcgaagc cgttgacacc cagaaagacc ttgttaacgt tgtacctgtt
     6541 caagaaggga ttaatctcaa aatcgagaaa gttcattgtg ttgtgctgga aagtgcctcc
     6601 tataaccact agatttatgt tcgggtactg aacgacagcg tttattatgt agagtgaatt
     6661 ggtgacaacg gtgagtatcg taggtgggtt cttgctgagc tcttcgaaaa agaaaagcgt
     6721 agtgcttcca gcatctataa aaattacgtc atacttctca acaaacttcg tagctttcaa
     6781 agcaatatgt cgtttttcat caacatcaat tgcaaccttt tcctggaatt cgggatcata
     6841 cctttgaagc tttgtctgga tggcaccacc atgaactctc tttagcaggc cctcttgctc
     6901 gagttcagag agatcgcttc tgatcgttac ctcggagacc cccagttcgc tggctatctc
     6961 agtggatcta acctttcctt taatagacaa ctctctaagt atctgtctgc gcctttcttc
     7021 agaaagaatt gtaatcgcct cctttgttga aactatacca ctccggattt ttgaaaagca
     7081 aattcaaaac aagccgaatt ttcgaattat ctgtctttat tttcgattat cttgttattt
     7141 ctattcgttt ctacagctta cgatttactg tatccgataa ataggcgaag aaataaataa
     7201 gtaaaaagaa aggaacacaa ctccatatag agtgtgtgtt tgcgaaaagc aaaacaaaac
     7261 gaaagagttc caaagaaacg ggagcaccag aggatcatag aaagcataaa aggcggaaca
     7321 tacataatcg actcgacacc gataaaggac atcacaagtc aggggataaa attaaagagt
     7381 acaatctcac atacctatct tctctcgccg agatcttctt tccattctcg atcttcctga
     7441 tatgaattct ccggcctcga cgagatagtc tttctatcca gagagctctg aggaccgggc
     7501 taaagccgaa atcgatatct ttcaagctca atttctgccg gttatagtat ctatagcctt
     7561 ttgaagagtg gcatcgccgt ttccgagata ccagttgatt tcctcttcca agctctgttc
     7621 gattacaaga tccggctcga tgtgctcccc ttcagctcca ttgataacta gaatcttcga
     7681 cagacagaag aggagacccg tattcggcat ggtgtggaac ggattggtta ttccgaagtt
     7741 gaccggctcg ctcgagcgct cgccaatcac cacggcgtcg gtgtttctcc gcatgaagct
     7801 caggaagaga agtgttgccg aattgacgtt ggcgttggtc agaatccata tcctgctatc
     7861 tttggctttc catgaataat cgggatagat aggcaggttc cacgtcctga tggtgtactg
     7921 gcccgatgaa tctcttgtgc cgatcttttc gacaaggtcg gccctctctt tcgtcagata
     7981 acccagaacc gcctccagga aaagctggaa tcccccgcag ttgtcccgga catctatgag
     8041 gagatcggag tatccatctc ccgggatccg atacataagc tcgagcgtct cttcctggta
     8101 atccggatga aagctcggaa tttttaggac cgctactgaa tcgatctgct cgaaggccgg
     8161 tgatctgttt gagtttctca tcatctgcca gtctgaatcg ctcaccgaaa cagaattgat
     8221 cgcagttttg agtat
//
LOCUS       DAOOLY010000094         8199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10260_length_8199_cov_3.917117, whole genome shotgun sequence.
ACCESSION   DAOOLY010000094 DAOOLY010000000
VERSION     DAOOLY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10260_length_8199_cov_3.917117"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..627
                     /locus_tag="PLD15_06165"
     CDS             328..627
                     /locus_tag="PLD15_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63524.1"
                     /translation="MRDFDYEGRLLMPEDNVLVELREVPYNAHIGDLLVTVVHRDRGR
                     LEAPEGWVLCSTRIDPDEVFSLYKSVFYHRVADMNETYTWELHGGSYLSVRQHQF"
     gene            640..1008
                     /locus_tag="PLD15_06170"
     CDS             640..1008
                     /locus_tag="PLD15_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63525.1"
                     /translation="MSVAEIPLEELYTDRLESLADIGRLQVALGMGLQVDQQGNPYQT
                     RLDTNVRIVRMIELELVRRQGYEATKAQLGDGPMAGMKLPPPSEDLMAKIAQAVSGIT
                     RELVEESFVTGGRSTKYGAD"
     gene            995..1342
                     /locus_tag="PLD15_06175"
     CDS             995..1342
                     /locus_tag="PLD15_06175"
                     /inference="COORDINATES: protein motif:HMM:NF023181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HPB63526.1"
                     /translation="MEPTSLPEGVDHPAHYNQHASGIECIDVVEGFNFNRGNAIKYIW
                     RAGEKGGTIRAEIQDLEKARWYIAREIERLQREADRIPGGDHGRTTIDVESGDDGSTP
                     SASPSTGFGSDHL"
     gene            1284..1667
                     /locus_tag="PLD15_06180"
     CDS             1284..1667
                     /locus_tag="PLD15_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63527.1"
                     /translation="MMVLLLVLLLALGLGLTISNGDGDPIGYARAEEAKAKASVDITQ
                     ILADLQGQIATLQAENDRLQIVLEYQDRAETRADTRQILNTVSMVALIISLAVFTLVL
                     AVAAGKFVGTQVQPTTKRRRHEDPR"
     gene            1651..1851
                     /locus_tag="PLD15_06185"
     CDS             1651..1851
                     /locus_tag="PLD15_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63528.1"
                     /translation="MKIHGNSPLAAMILFAIAISTTMLLMILITYVARGQSLEVEPTP
                     TPIFAPEPVIEFHNLYLAYVAG"
     gene            complement(2123..2347)
                     /locus_tag="PLD15_06190"
     CDS             complement(2123..2347)
                     /locus_tag="PLD15_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63529.1"
                     /translation="MNEENNERTDLKTLMNLGDDCAFLKTFRVGGQKLIIDSPTAQTP
                     QCSELRSSSGSSSMLLIALLLTILKIETDE"
     gene            2362..2790
                     /locus_tag="PLD15_06195"
     CDS             2362..2790
                     /locus_tag="PLD15_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63530.1"
                     /translation="MLELDLLLLAESHDTDCRCPVCFRFWQEEGPSEIDDNGVADYGP
                     FTTEEIEAASLERLLQGIDMSGLQSPNYTQGAFDYLSALARVPELSATSSTLGGAEHL
                     YARCDYCDREGPDVTHRVIERGSNVEEAWLCDDCYSDART"
     gene            2849..3397
                     /locus_tag="PLD15_06200"
     CDS             2849..3397
                     /locus_tag="PLD15_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63531.1"
                     /translation="MAATKPAVQEIDWSGLLDQFASQSGGGKVFYLKPGKNRIRMLPP
                     KGKNPFISVETSYRGRTRTKYLVGAVVPDAIDPERNVDDVGIRAVLLTRQQIHTILTN
                     QTEGFDLFSPAGNGLTIIKAGSGLNTTVTISVTPKPIVIADEVLEAWKEFDLEAVAKD
                     WIAYQKSRAEQGTNGETEEDED"
     gene            3467..4069
                     /locus_tag="PLD15_06205"
     CDS             3467..4069
                     /locus_tag="PLD15_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63532.1"
                     /translation="MNVNEFIPTIKASDYGLDDLIDFRESDFLGFCARESQNGGIDPT
                     VPLYMPKGQENVRVLFARTVEEILESLDASDEIHRQEELIDAWNFALSMLFLNTGTPA
                     PRIKDYLIVAVEKYRRDGVPSKDVVAGIKPFTELLDKLRNRSWQNNSQHPVFNGTPQL
                     VRALAKFHLWVEGQFETPDDFTVMNVAKHKVLEFRLQTNY"
     gene            4080..4760
                     /locus_tag="PLD15_06210"
     CDS             4080..4760
                     /locus_tag="PLD15_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63533.1"
                     /translation="MAIPINQLFQLILEEHQSQRTLHVTTYRPIEVELPDQSNAFSDY
                     EWGDFFDSVDSCIPGTLLGRVRYLTSNAHYKPRWDSPGRTHATFGNGCLRSYNRPLRT
                     LTWAKRSEPWCKLFSDMLYLRAVVDPDLQDAPHVVTFHLEKGSIFKNRHLPTLYYWAQ
                     KYGYATLPPFVRQRAEYHIEKLLEKLKRDRPISYFSVEAKWQVKYLELAGILDDQQLQ
                     YHLSLGKE"
     gene            4911..5543
                     /locus_tag="PLD15_06215"
     CDS             4911..5543
                     /locus_tag="PLD15_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63534.1"
                     /translation="MKAAGIPYTPAELVEVVAPILPFWEQNRQAMINWLGRELDVRIG
                     GFLGMDGLMGISTEQSAESYHPELAKYFEGNEASYYYMDRLYGGAPAMIKALSQEGSR
                     RAYWPIFREEDAIRAHRLTRIPCTLGYNWLIRPSTFLGSGWIMDAGIQQRSCDFDKFM
                     ISDLWFTYRLTTALAENFPVDHSFYDADVELDMVYHHAISLHAFIDSEIF"
     gene            5924..6442
                     /locus_tag="PLD15_06220"
     CDS             5924..6442
                     /locus_tag="PLD15_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63535.1"
                     /translation="MRTPKVGVDLALRKGGIAFIDPTGQAVVGSFDIGSGTTKLSVPQ
                     FIQRYEKTLIPKLLGYPADSQFYVDWSPNEFQFGGRTSLRAVSMKSFLAGYLYQRLIR
                     ERRQIVFLTPQEVRAALGFPKNLKKELVHVRFVQEFRLPDSITRNFTNWSPDSRDAYI
                     LAYVGGLLEEDD"
     gene            complement(6994..7407)
                     /locus_tag="PLD15_06225"
     CDS             complement(6994..7407)
                     /locus_tag="PLD15_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63536.1"
                     /translation="MSHENHERLVESVESDVERLEAGSPLLDGLIDSVGGDVDLGKRR
                     GVTEGLRRDVHHETIGLELLGARCVVSCVENARQDRLRFIDRTFAKRGTSVEQLSELS
                     DVTSGSNAEVKTGEFVGEGEIPEKTFRYPNLVGCD"
     gene            7399..8022
                     /locus_tag="PLD15_06230"
     CDS             7399..8022
                     /locus_tag="PLD15_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB63537.1"
                     /translation="MAQPGTGKTSITLSVAEELVKQGTQCWAFETELPAQVLQGRMAP
                     MLRRTSFASTGSRIFCGPYSIDDVIEMVKEDPNPNRIIIFDGPDAMFVKSSTGERRFD
                     LEQIYMKLVILKHMCKAVFATSQPRRNDQELKITSVAESWSKSWLADVLVGIQRHGPS
                     TLMLSVLKNRFGPGGGRIFFNYNYETMEWDLTAAGHLNAADEDWDDL"
BASE COUNT         1830 a         2320 c         2222 g         1827 t
ORIGIN      
        1 acacttcgcc ttcttcctct ccggtcgact cgaaggctcc tacacgaact ggtaatcgac
       61 tcaacgctca cacaacttcg tctccaacca acggggacga agttttattc taggaggact
      121 caccgatggc caccacacta cgttctctct cagccgctca gcgtgctgcc ttaatcaatc
      181 tatcacggga gaagtggatc tcagcctatg aggctcgagc cactctcaag acgctcgagg
      241 ccctgaagaa gaaaggtcta gttcgagacc aggctcaggt cggctacttc tcgatgccga
      301 ggacagccat cgaatggatc ctgaccgatg agggacttcg actacgaagg gaggctctta
      361 atgcccgagg ataatgttct ggtcgagtta cgtgaggttc cttacaacgc ccacatcggt
      421 gacttactgg tcaccgtagt ccatcgagac agagggcgtc tcgaagcccc tgagggttgg
      481 gttttgtgct caactcggat cgaccctgat gaggtcttca gcctctacaa gtcggtcttc
      541 taccacaggg ttgccgacat gaatgagacg tatacgtggg agctccacgg tggttcctac
      601 ctcagcgtcc gacagcacca attctagaag gagaaaaaga tgagcgtagc ggagattcca
      661 ctggaggaac tatacacaga tcgacttgag agcctggccg acatcggtcg acttcaagta
      721 gcgctcggga tgggcctaca ggtcgaccaa cagggtaatc cctatcagac caggttggac
      781 acaaacgtcc ggattgttcg gatgatcgaa ctcgagttgg tccgtcgcca aggttatgag
      841 gcgacgaagg ctcaactggg tgacggtccg atggccggga tgaaacttcc accgccgagc
      901 gaagacctga tggcgaagat cgcccaagcg gtctcgggaa ttactcgaga gctcgtggaa
      961 gagtcgttcg tgacgggcgg gaggtcgact aagtatggag ccgactagtt tgccggaagg
     1021 cgtcgaccac ccggctcact acaaccaaca cgccagtgga atagagtgca tcgacgtggt
     1081 tgagggcttc aacttcaacc gaggtaacgc catcaagtat atctggagag ccggcgagaa
     1141 gggcgggacg atccgcgccg agatccaaga ccttgagaag gcgaggtggt acatcgctcg
     1201 tgagatcgag cgacttcagc gagaggctga tcgaatcccg ggaggggatc atggaagaac
     1261 aacaatcgac gtggagtccg gggatgatgg ttctactcct agtgcttctc ctagcactgg
     1321 gtttgggtct gaccatctct aatggtgatg gagatcctat aggctacgct cgagccgagg
     1381 aggccaaggc caaagcctcg gtcgatatca ctcaaatcct ggccgatctc caaggccaaa
     1441 tcgctacact acaagcggag aatgatcgcc ttcagatcgt gctcgagtat caggaccggg
     1501 ctgagacacg ggccgatact agacagatac tgaacacggt gtcgatggtc gcgctgatca
     1561 tctctctagc cgtgtttaca ctcgtactgg ccgttgcagc cggtaagttc gtcggaacgc
     1621 aagttcaacc aaccaccaaa aggagacgcc atgaagatcc acggtaactc gcccttagcg
     1681 gccatgatcc tgttcgccat cgccatcagc acgacgatgc tcctgatgat tctgatcact
     1741 tatgtcgcac gtggacagag cctcgaggtc gagcctactc cgaccccgat cttcgcacct
     1801 gaaccggtga tcgagttcca caatctctac ttggcctacg tcgccggata atcatgtcga
     1861 agaaaagata ctcaacgatg tgcgacgacc aggaatgtct ctgtcactgg attgagggcc
     1921 acgttcactg tactacgtgc ggtgctctca tcgacgtctc tggccctgcc ggtctagaca
     1981 tccatcgctg tccgatgaag gagcccatgg agaaagcgaa gccgattacc tacgaagaag
     2041 atggagacgt cctatgggct tgactccata ccagtggctt accctgatcg gtagctcgct
     2101 cggtgccatc gctatcgtcg gactattcat cggtctctat cttcagtatc gtcaggagaa
     2161 gcgctatgag taacatggat gaggatccgc tggacgacct taattctgaa cattggggcg
     2221 tctgcgcagt cggtgagtcg attatcaact tctgtcctcc aactctgaag gtcttcaaga
     2281 aggcgcagtc gtcgccgagg ttcatgaggg tcttcaggtc cgttcgttcg ttgttctctt
     2341 cgttcacagg agctgcttcg tatgctcgaa ctcgatctgt tgttgctggc tgagagccac
     2401 gataccgatt gtcgctgccc ggtttgtttc cgcttctggc aggaagaggg gccgagcgag
     2461 attgacgaca atggcgtggc tgactatggc ccgttcacca ccgaggagat cgaagcagcc
     2521 agtcttgaaa ggttacttca aggtattgac atgtctggtc ttcaatctcc aaactacact
     2581 cagggcgcct tcgactattt gtccgcactc gctcgtgttc ctgaactgtc ggcgacttcc
     2641 tcgactctcg gtggtgccga gcacctctac gctcgctgcg actactgtga tcgagaaggt
     2701 ccagacgtca cgcatcgggt tatcgaacgt ggttcaaacg tcgaggaggc ctggctctgt
     2761 gacgactgtt acagcgatgc cagaacataa gtcgtatcaa caatcgcaat cgcagctatc
     2821 gcatttgcac taagaaagga cagtgactat ggccgctact aagcccgcag tacaggagat
     2881 cgactggtct ggtcttcttg atcagttcgc ctctcagtcc ggcggaggta aggtctttta
     2941 cctcaagccg gggaagaacc gcattcggat gctgccgccc aaggggaaga acccgttcat
     3001 ctctgtggag acgtcgtacc ggggccgcac tcgcacgaag tatctggtcg gcgccgtggt
     3061 tccggacgcc atcgaccctg agcgcaacgt ggatgatgtc ggtatccgcg ccgtgctctt
     3121 gactcgccag cagatccaca ccatcctcac caatcagacc gagggcttcg acctcttctc
     3181 tccggcgggg aacggtttga cgatcatcaa ggcaggctct ggcctcaaca cgacggtgac
     3241 catctctgtg acgccgaagc ccatcgtgat cgctgatgag gttctcgagg cgtggaagga
     3301 attcgatctc gaggccgtcg ccaaggactg gatcgcctat cagaagagcc gggcggaaca
     3361 gggcaccaac ggcgagaccg aagaagacga agactaggat cgactctact cactcgaaag
     3421 ggccgggctc ctgcccggcc tcacttttag aaaaggacca acctcaatga acgtgaatga
     3481 gtttattcca accatcaagg cgagtgacta tggcctggat gatctgatcg acttccgaga
     3541 atcggacttc ctcgggttct gtgcacgtga gtcgcagaac ggcggtatcg acccgaccgt
     3601 tcccctctat atgccgaagg gtcaggagaa cgtccgtgtt ctcttcgctc ggaccgtgga
     3661 agagatcctc gagtcgctcg acgcttcgga tgagatccat cggcaggagg agttgatcga
     3721 cgcctggaac tttgccctct cgatgctctt cctcaacacg ggtactccgg cgcctcggat
     3781 caaggattat ctcatcgtgg ctgtcgagaa gtatcggcga gatggggtac cttcgaagga
     3841 cgtggtggcc ggtatcaaac cgttcaccga gctcctcgac aagctgcgca atcggagctg
     3901 gcagaacaac tcgcaacacc cggtgttcaa cggaactccg caacttgtgc gtgcactcgc
     3961 aaagttccac ctctgggtcg agggtcagtt cgagacacct gatgacttca cggtgatgaa
     4021 cgtcgccaag cacaaggttc tcgagttccg tctccagacg aactactagg cggcgggcga
     4081 tggcgattcc gatcaaccaa ctattccaac tcatactcga agagcaccag tcacagagga
     4141 ctcttcacgt gacgacctac cggcccatcg aggttgaact tcctgaccag agcaacgctt
     4201 tctcagacta tgagtggggc gacttcttcg actcggtcga cagctgtatt ccgggcacac
     4261 ttcttggacg tgtccgctac ctcacttcta acgctcacta caagccccgg tgggattccc
     4321 cagggcgtac ccatgcgacg ttcggtaacg gctgtctacg gtcctataac aggccattac
     4381 gcacgcttac gtgggctaag cgtagtgaac cttggtgtaa gttgttctcg gacatgctct
     4441 atctccgcgc cgtggtcgac cccgacctac aagatgctcc gcacgtcgtg accttccacc
     4501 ttgagaaggg ttcgatcttc aaaaaccgcc acctgcccac tctctactac tgggcgcaga
     4561 agtacgggta cgccacactt cctcccttcg ttcgacaacg ggccgagtac catattgaga
     4621 agctccttga aaaactcaag cgggatcgac cgatctcgta cttctcagtt gaggcgaagt
     4681 ggcaggtaaa atacctcgag cttgccggga ttctcgacga tcaacaactt cagtatcatc
     4741 tgtctctagg aaaggaataa cactctatgc gaattttccc aactgtccgt gaagcactgc
     4801 tcgagatccg gcgtgatgtt tacaagtcac ccgaggttcc gagctcccgt gtccagagtg
     4861 tcgtcaccga gcatggccgc actcacgaag ccgtgtacta ttcctacggt gtgaaggcgg
     4921 cgggtatccc gtacacgcct gcagagctgg tggaagtcgt cgctccgatc cttccgttct
     4981 gggaacagaa tcgtcaggcg atgatcaact ggctgggtcg tgagctcgac gttcggatcg
     5041 gcggcttcct cggtatggat ggcctgatgg ggatctcaac cgagcagtcg gctgagtcgt
     5101 atcacccgga gttggccaag tacttcgagg ggaacgaggc gtcctactac tacatggacc
     5161 gtctctacgg tggcgctccg gcgatgatca aggcgctctc tcaggaaggg tctcgccgcg
     5221 cctattggcc gatcttccga gaagaagatg cgatccgggc acaccgcctg actcggatcc
     5281 catgcacgct cggctacaac tggctgatcc gtccgtcgac cttcctgggc agcggctgga
     5341 tcatggacgc cgggatccaa cagcgctcgt gcgacttcga caagttcatg atcagcgacc
     5401 tttggttcac ctaccggctg accacagcgc tggctgagaa cttcccggtt gatcattcgt
     5461 tctatgacgc tgacgtcgaa ctcgacatgg tctaccacca cgccatctcg ctgcacgctt
     5521 tcatcgactc cgagatcttc tagggtggct ctctcaaaac ttggccttac ggaggccgaa
     5581 cggacagcgc tggttgaact cctcgaggga acgacagatc cgctgttaca gaatgtcctc
     5641 cgtaaggcca aggacgcagc cgtcgtgaag aagacccgga agagggtgcg acctcgctac
     5701 tcagaagttg atgaggccat cggactttct cactgcggct tgctgttagc gtggacaatg
     5761 atcgaggaag gtccttccgg ctggcgagtc gtcggacact gtactcgctg caacgaacag
     5821 atctatcggt ccaagcgggt tgtaacctat ctgcgtgctc gggtaaccgt tgatcagata
     5881 gatcgactga acagaggctt aggccttagg aagagtgtac ttgatgagga ctcctaaagt
     5941 aggcgtagac cttgctctac gcaaaggtgg aatcgctttc atcgacccaa ccggacaagc
     6001 cgtggtaggc tccttcgaca tcggctcggg aaccacaaag ctgtccgtac ctcagttcat
     6061 ccaacgctat gagaagacac tgatcccgaa gttgttgggc taccccgcag actctcagtt
     6121 ctacgtcgac tggtctccga acgaatttca gttcggaggt cggacgtcgt tgagggctgt
     6181 ttcgatgaag tccttcctag ccgggtacct ctaccagcgg ctgattcgtg aacgtcgaca
     6241 gatcgtcttc cttactcctc aggaagttcg agctgctctg ggttttccaa agaacctgaa
     6301 gaaggaacta gttcacgtcc gatttgttca ggaattccgg ttgcccgata gtatcacacg
     6361 gaattttacg aattggtcac ccgatagtcg agacgcctat attctcgcct acgtgggtgg
     6421 actgttagaa gaggacgatt aaaccgtgca gacctttctg ccatactctg attttcaccg
     6481 tagcgctaag gtccttgacc gtcagcggct cggtaaacaa agagtcgagg ttttgcaaat
     6541 ctataaggcc ctctcctccg gcggtggttg gtctaaccat ccagccacgc taatgtggaa
     6601 agggtacgat tacgcactac tcgagtatgg cctagtcatc tgtcaggaat ggaggcgtcg
     6661 aggttataac gactcactgt tgccgcggtt cgagcgagag cttgaatcgc tctacccaac
     6721 gggttatcca gagtggttag gtatagagct gttacacgac tcacacaaag cacacttata
     6781 ccacaaggac cctgcctatt acttgatgtt tgcgagttac tcgacaagga tactactatg
     6841 gcccaaaacg atcatgacga cctctgggat ggaggtcaag tgcttggtac cgtcggatcc
     6901 gctccggatg gatctcaacc cgccgtcggt cacgcaaaga agtcccgacc caactttgca
     6961 ctaggtgtcg atgacttcag tcgactggtt tacctagtcg caacccacaa gattcggata
     7021 ccgaaaggtc ttctccggta tctccccttc tccgacgaac tctcccgtct tcacctcggc
     7081 gttggagccg ctggtgacgt cgctcagctc gctcaactgt tcaacgctgg ttcctctctt
     7141 ggcgaaggtt ctgtcgatga agcgaagacg atcctgtctc gcattctcga cacacgagac
     7201 tacgcatcga gctcccagaa gctcgaggcc tatcgtctcg tgatggacat ctcgtctcag
     7261 gccttcggtg actccacgcc ttttgccgag gtcgacgtct cccccgacgc tatctatgag
     7321 cccatccaga agtgggcttc cggcttccaa cctctcgaca tcgctctcga cggactctac
     7381 cagtctctcg tgattttcat ggctcaaccc ggtacgggta agacgtcgat taccctatca
     7441 gtcgctgagg agctcgtcaa acaaggtacc caatgttggg ccttcgagac ggagttaccc
     7501 gctcaggtac ttcagggtcg gatggctccg atgctccgcc gcacttcctt cgcctcgacg
     7561 ggttctcgga tcttctgcgg gccctactcg attgatgatg tgatcgagat ggtcaaagag
     7621 gatcctaatc ccaatcggat cattatcttc gatggcccgg acgccatgtt cgtcaagagc
     7681 tcgactggtg agcgacgctt cgacctcgag caaatctaca tgaagctcgt aattctgaag
     7741 cacatgtgta aggcggtctt cgctacttcg cagccgaggc ggaacgacca ggaactcaag
     7801 attacctccg tcgccgagag ctggtcaaaa tcgtggttgg cggatgttct cgtgggtatc
     7861 cagagacacg gtccatcgac gttgatgctc tccgtcctca agaatcggtt cggtccaggc
     7921 ggcggtcgca tcttcttcaa ctacaactat gagactatgg aatgggacct caccgccgcg
     7981 ggccatctca acgctgccga cgaagattgg gacgatctct aactcggccc gatttgccct
     8041 tacaaccgac aacgagaacc ggagttctgc tccggttttc ttttacctag aaaggatcgt
     8101 cggtgaacac aacacgaagg aaaatataca aacgctgtcc gtactgcggt gactcgcaga
     8161 agaacccgga caagcgccac atggccgtct tcgtagact
//
LOCUS       DAOOLY010000095         8178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10293_length_8178_cov_3.980180, whole genome shotgun sequence.
ACCESSION   DAOOLY010000095 DAOOLY010000000
VERSION     DAOOLY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_8178_cov_3.980180"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLD15_06235"
     CDS             complement(<1..292)
                     /locus_tag="PLD15_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPB63538.1"
                     /translation="MTHVHLLERDRALKLEIADITVQEVDAIINPANAYLRHGGGVAG
                     ALVRAGGREIQLESDALVRTDGPLEVSGVAVTGAGTLKVGKIIHVLGPRLGEK"
     gene            complement(289..1599)
                     /locus_tag="PLD15_06240"
     CDS             complement(289..1599)
                     /locus_tag="PLD15_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPB63539.1"
                     /translation="MSISETNWKPLSERIRPRTFDDLVGQEHLAGKKGIIRGAVESGR
                     LFSMILYGPPGTGKTTIGELIRAYLGNNYHFEFFSASLQGTADLKKHFSHGERLKAVG
                     QQLVLFVDEIHRLNKSQQDVFLPVTERGTIVLIGATTENPSFEVNPALLSRCRLVILK
                     QLNAADILRLLSRALERDTFLSTSGVSVDNEVLEVISGSSGGDARIALNLLDTLVESA
                     AALEREKLDLELMNELSVLPSGRYTKKGEEHYDIASAFIKSMRGSDPDAALYYMIRML
                     EGGEDPKFIARRMVIFSSEDIGLSDPMALVIAISAFEAVNSVGLPECILNLSEAAIYL
                     SVATKSNRVNEAMMTTRDRVRNTPNLPVPLKLRNAVTNMMKGLGYGKDYHYPHDSGGF
                     SKEYYLPEEIKQEIYYKPGNMGKERQVAERLRTLWKGLKEYPEE"
     gene            complement(1562..2506)
                     /locus_tag="PLD15_06245"
     CDS             complement(1562..2506)
                     /locus_tag="PLD15_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB63540.1"
                     /translation="MGENYLVTGGAGFIGSHVVDALIRDGKIPIVVDNLSSGKIENLD
                     PRALFYQQDITDCEMMERIFMLHKPKAVFHLAAQISVSKSVREPEEDARVNIIGTIRL
                     LQMAIKYGVRKVIFSSTGGAIYGDDVKKIPTDENEFPKPLSPYGIAKFSTENYLRFFA
                     GEHGFKYATLRYANVYGPRQDPHGEAGVVAIFSRRMLKGENVVIFGDGECVRDYVYVE
                     DVARANLLAMERLENDTVNIGTAIGTSVNGLFEKMKVTSGYSKEAIHEAPRPGDLKKS
                     ILNYSKASTLLDWEPSVPLEKGLEKTIEYFRNELETLK"
     gene            complement(2508..3137)
                     /locus_tag="PLD15_06250"
     CDS             complement(2508..3137)
                     /locus_tag="PLD15_06250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB63541.1"
                     /translation="MTDYVKRKEHLLGVISKINDCTRCPLSTTRNNVVPGEGSIQSPI
                     MFVGEGPGADEDASGRPFVGKAGQLLTKILESVKIDRQDVYITNIVKCRPPNNRVPTT
                     EEKNACSPYLLSQIAIVSPKVIVALGATALSFFVSDEKIRMTEARGKLFDWLGGIKIF
                     VMFHPSYLLRYPSRDPGSPKSLTWEDIQNIRKMYDRLIEGREIELQGGG"
     gene            complement(3149..7501)
                     /locus_tag="PLD15_06255"
     CDS             complement(3149..7501)
                     /locus_tag="PLD15_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPB63542.1"
                     /translation="MGTLIKYIGYQTLGSFFIGLGGFIVFVSLELLYYLSDMIIRYKV
                     GIDKLFLLIYYNLPEFIVMGVPVGVLLGIFWVLSRMRSDNELIALQTHGISLKKLVIP
                     FLILGVVFSGVAYLLNDYLVPNASSKATEAMARFVYRQPEVTLKENVFMQDNQGRMIH
                     VRRIDPETKELKGVSIYEVTRSEVTLTTATSAVISPNKWTLKDVRIYQVDSSGFLGAE
                     MQFNTAEFDIDQDIERYLASFKAPKEKTSAELRQDIKAFKNTGIDTSSLEVALQEKYS
                     MSIAPLVIVLLGVPVSLMFNLQSKAWSVILTFLLVVIYQGSGAWLSGMGKENLLNPTL
                     APWIPNIVFAIVGVIVYSLIDTKASYRLSEMLTRLLRISVILLAIIVPGTLIGANVKI
                     VGGSILGSGMGNEILLQDGVRVDYSSETLTATIDASNASILTSNGSPVSISFWGNVTM
                     VSSETTINSERLILDLTNEKLESMEIYTRTEIEVPAVRGEGSGDGSKVPFYVYGSYIQ
                     STIEASPTMNVTDGYITTCDRGHPHYRFKVSYAVVTPGKGMSVQNMVMYIGDLPVFYL
                     PYYYFPLDDPDRRPFDVDLSGIVDAKTRITLRYLELDWLLLQGSWYRNWLSTEDAFTA
                     KAALYTPFGDLELFGQFGQEKQTSYGGYVLIKPKLEWMRFQGGALYITGPSLKELQTP
                     FTGLNLGETITSRKVTQMDPFAIKQSMNATELTLGYVQFLSPEEWDVELDVFGAYARL
                     DDKTLWMVNLQKLTIPTEEGFDAGFFKFKTSEFNLSGLFGQRYINKEILYSLRTKASA
                     IQTEGELFDADFSVKSLNFSLASAAATITELFDPAKMEDFVLEVSTYLFTLGNLKIGG
                     DLKGTFDASTTQLNITMPADNKGIGKNYLTFVTDDGKLKINGRYALDFDSQHEKNKDR
                     VFWIDPLDLLYEDFVTVELKFYFESRYDGSFKIEGKKLRVYKDFELLKKEWGPVSLNV
                     ILEPAYEIAFEYESGSDFKLTKNQIPITLKNEVMWKPLEWYYLGVQYNPVLTYDFLKS
                     EWRLDHPGKLLTGINTEALKIDYSLELDYEELVASPTDMNWLGKGELNSEGKFDLWGT
                     SLSHKSDTIFMPVTSKASETSYSVSFESDIFSHSTENKVYWQSDDLFDDFVNKEKLTL
                     SLTTKTKLEFTLDWTYDASPNIVDNERRLKDLVFGSSITLEDFATFALKFRYPYLSGK
                     NKIYDRIRVEVNNLTVGEVKWKSAFLDFTTGFSTFDSKYKFPAKYFSAAETRARFAVW
                     ESKQLEISSLTVGEYFIATKTASNTNYSAYSLGFSNVKYGNSTVLGGSTGKFEEFGMS
                     MELAFSDDIDFVMHINDLYFPMGSLSDKPGILKRFSFGIDGSGGRNFVEIGTYAGDIA
                     LWDESMSKISPLYLDLHCMAAEIILKISFGSQVKTFTETLETIAVKFYIKELKDRYLV
                     IGLHKGAPFFRFRF"
     gene            complement(7519..8175)
                     /locus_tag="PLD15_06260"
     CDS             complement(7519..8175)
                     /locus_tag="PLD15_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HPB63543.1"
                     /translation="MLRSDILKMARYIQNICNKSSIAMLERNEHQARDVLYSDSVIDH
                     YQIELQKTIISLTGILTPTGKDLRFLTMTMSIVNTLERIGDKFVSICELAMVLIKKPP
                     IGYYEKLRTMFDGVSLMLKGSIDNLVDPSLLEAERLCRNDSNIDSLLEGVRNDLLSNM
                     EQSPSITSRAVDLLRVFCDLEEIADLCTELMEAAVYIDTGKYYRCMNDIFQPVDFKAY
                     "
BASE COUNT         1893 a         2111 c         1926 g         2248 t
ORIGIN      
        1 ctttctcacc aagtcgtggc ccgagaacgt gtatgatctt gccgaccttc agagtgcctg
       61 cacctgtcac tgcaactccc gagacttcca aaggcccatc ggtcctcacc agggcgtcgc
      121 tttccagctg aatctctctg ccgcctgccc tgacaagcgc tccggcgaca ccgcctccgt
      181 gtctgagata ggcgttggcc gggttgatta tcgcatcgac ctcctggacg gttatgtctg
      241 caatctccag cttcaatgcc ctatcccttt ccagcaaatg aacgtgcgtc attcctccgg
      301 atactccttc aatcccttcc agagggttct caatctttcg gccacctgcc gctctttgcc
      361 catgtttcca ggtttgtagt agatctcctg tttgatctct tcgggcaagt aatattcctt
      421 tgaaaagccg ccactatcgt gcggatagtg gtaatccttt ccgtagccga gtcctttcat
      481 catgttcgtc acggcgtttc tgagcttgag aggcaccgga agattaggtg tgtttctgac
      541 ccgatcgcga gtcgtcatca tggcttcgtt cacccggttg ctcttggtcg cgacagacag
      601 ataaatcgcc gcttccgaga ggttgagaat acattcgggc aatcccacgc tgttgacggc
      661 ttcaaaggcc gatatggcaa tcacaagggc cattgggtcg gagagtccta tatcctcgga
      721 ggaaaagatc accatgcgtc gggcgatgaa ttttggatct tctcctcctt ccagcattct
      781 aatcatgtaa tacagtgccg cgtcagggtc gctccccctc atgctcttga tgaaggccga
      841 tgcaatatcg tagtgctctt cacctttttt ggtgtaccgt ccagaaggaa ggaccgaaag
      901 ctcgttcatc agctctagat ctagcttctc tctttcgagc gcagcggcgc tctccactag
      961 agtatccagc agattgaggg cgatacgggc atctcccccg ctgctgcctg agataacttc
     1021 tagaacctca ttatctaccg atacgccgga ggtagataga aaagtgtctc gctcgagggc
     1081 acgcgacagc aggcgcaata tatctgccgc gttcaactgc ttgaggatca ccagacggca
     1141 acgcgacagc aaggccggat tgacttcgaa actggggttc tccgtcgtcg ctccgatgag
     1201 tacgatcgta ccccgctcgg taacgggtaa aaagacgtct tgctggctct tgttcaatct
     1261 gtgtatctca tctacgaaga gaaccagctg ttgaccgacg gccttcaacc tctcgccgtg
     1321 tgagaagtgt ttcttcagat ctgccgtccc ctgaaggctg gcactgaaga actcgaaatg
     1381 gtaattgttc cccagatagg ccctgatgag ctcacctatg gtggtttttc ccgtgcctgg
     1441 gggcccgtaa agtatcatgg aaaagagccg accgctttcg accgctcccc tgattatccc
     1501 tttctttcca gccaggtgtt cctgtccgac caggtcgtcg aaggtgcggg gcctgatcct
     1561 ttcacttaag ggtttccagt tcgtttctga aatactcaat cgtcttttcc agtccttttt
     1621 ccagcggcac cgaaggttcc cagtcgagaa gggtgctcgc cttggagtag ttcagtatcg
     1681 atttttttaa atctcccggt ctcggagctt catgaatcgc ttctttggag taacccgagg
     1741 tcactttcat cttttcgaaa agcccgttga ccgaggttcc gatcgccgta ccgatattca
     1801 ccgtgtcgtt ttccagtctc tccatggcca gcaggttggc ccttgccaca tcttccacat
     1861 acacgtaatc cctcacgcac tcaccgtcgc cgaagatcac cacattctca cccttcaaca
     1921 tcctccgcga gaagatggca acgactccag cctcgccgtg aggatcctgt ctgggtccat
     1981 agacgttggc gtatctgagc gtggcgtact taaatccgtg ctcgccggcg aagaaccgca
     2041 ggtagttttc tgtggagaat ttggctatcc cgtagggaga tagaggtttg ggaaattcat
     2101 tttcatcggt cgggatcttt tttacgtcgt ccccgtatat ggctccaccg gtggaagaga
     2161 agatgacttt tctcactccg tacttaatcg ccatttgtag cagtctgatc gtaccgatga
     2221 tgttgaccct cgcatcttct tccggttctc tcaccgattt cgatacgctt atctgtgctg
     2281 cgagatggaa tacggcttta ggtttgtgga gcatgaaaat tctctccatc atttcacagt
     2341 cggttatatc ctgctggtaa aaaagcgccc tcggatcgag gttctctatt tttccactcg
     2401 aaagattatc gacaactatc gggatcttac cgtctcttat aagcgcatct actacgtgag
     2461 agccgatgaa acctgcgcca cccgtaacaa ggtaattctc gcccatatca cccacctcct
     2521 tgtaattcta tttcccttcc ctctattaat ctatcgtaca tttttctgat gttctgtatg
     2581 tcttcccagg taaggctctt cggagagcca gggtcccggg aaggatatct taaaagatag
     2641 ctcggatgga acataacgaa gatctttatg ccgcctagcc agtcgaacag cttgcctctc
     2701 gcctccgtca ttcttatctt ttcgtcgctc acgaaaaagg acaaagccgt tgccccgagg
     2761 gcaacgatta ctttcggact gacgatagct atctgggaga gaagataagg tgagcaggcg
     2821 tttttctctt cggtggttgg aactctgttg ttgggtggac ggcacttcac gatgttcgtt
     2881 atatatacgt cctgtctatc gattttcacc gattctagaa tctttgtgag taactgccct
     2941 gccttcccta cgaacggcct tccggaagcg tcttcatccg cgcccggacc ttctcccacg
     3001 aacattatcg gagactgaat ggaaccctct ccgggcacca cgttgttgcg tgtcgtgctc
     3061 aggggacatc gtgtacaatc gtttatcttg cttataactc ctagcaggtg ttccttcctc
     3121 tttacgtaat cagtcacagc ttatcacctc aaaacctgaa tctgaagaaa ggcgctcctt
     3181 tgtgaagtcc aatgaccagg tatctgtctt tcaattcctt gatgtagaat ttcactgcaa
     3241 tcgtctccag cgtttccgtg aacgtcttca cctgacttcc gaaactgatt ttcagaatta
     3301 tctcggcggc catacagtgg aggtctaggt agagcggcga gatcttgctc atcgattcgt
     3361 cccacagggc tatatctccg gcgtaggttc ctatctccac gaaattccta ccgccgctgc
     3421 cgtcgattcc gaaggagaaa cgcttcaata taccgggttt gtctgataga gagcccatcg
     3481 ggaagtacag atcgtttata tgcatcacaa aatcgatgtc atcgctgaag gctaattcca
     3541 tgctcatccc gaattcttcg aactttcccg tgctgccccc cagtacagtg gagttgccgt
     3601 acttcacgtt agagaatccc agcgagtacg cactgtagtt tgtattggaa gccgtttttg
     3661 ttgctataaa atactctccg accgttaaag aggatatttc gagctgcttc gattcccaga
     3721 ccgcgaaacg cgccctggtt tcggcagcgc tgaagtattt ggccgggaac ttgtatttag
     3781 agtcgaaagt cgagaaaccg gtcgtgaagt ccaggaaggc gctcttccat ttgacctctc
     3841 cgacggtgag gttgtttacc tcgactctga ttctatcgta tatcttgttc tttccagaca
     3901 gataaggata tctgaacttc agcgcgaaag tggcgaagtc ttccagcgtt atggaggatc
     3961 cgaaaaccag atctttcagc cttctctcgt tatcgacgat atttggcgaa gcgtcgtaag
     4021 tccagtccag agtgaactcc agtttggtct ttgtcgttaa cgacagagtc agcttttcct
     4081 tattcacgaa gtcatcgaag agatcgtcag attgccagta taccttgttc tcggtactgt
     4141 gggaaaagat atccgactcg aagctcaccg aatatgaggt ctccgacgct tttgaagtta
     4201 ccggcatgaa aatggtatct gacttgtgac tgagagaggt tccccagaga tcgaatttcc
     4261 cttcgctatt caactctcct tttccaagcc agttcatatc cgtaggacta gcgacgagtt
     4321 cttcataatc cagctcgagc gagtagtcga tctttagagc ctccgtgttt attcctgtca
     4381 ggagcttgcc tggatggtcc agtctccatt cactcttgag aaaatcgtac gtgagaacgg
     4441 gattgtactg aactccgagg tagtaccact caagaggctt ccacatcact tcattcttca
     4501 acgtgatggg tatctggttc ttcgtcaatt tgaagtcgct tccagattcg tactcgaatg
     4561 ctatctcgta ggccggctcc agaatgacgt tgagagaaac gggtccccac tcttttttta
     4621 gcagttcgaa atccttatag actctgagct tctttccttc tatcttgaaa gagccgtcgt
     4681 acctggattc gaagtaaaac ttcagttcaa ccgtaacgaa gtcttcgtag agaaggtcga
     4741 ggggatctat ccagaaaact ctatctttgt tcttttcatg ttgagaatcg aagtccaggg
     4801 cgtacctgcc gtttatcttc agttttccat cgtcagtcac gaaggtcaga tagttctttc
     4861 caatcccttt gttgtctgca ggcatcgtta tattcaactg cgttgtcgat gcgtcgaagg
     4921 tacctttcag atctcctccg attttcagat ttcccagtgt gaaaagataa gtgcttacct
     4981 ccagaacgaa atcctccatt ttggccgggt cgaacagttc ggtgatagtg gccgccgcgg
     5041 aagccagcga gaagttcaac gacttaaccg agaaatccgc atcgaagagt tcaccctcgg
     5101 tttggattgc cgaagccttg gttctcagag agtacaggat ctctttattt atgtatctct
     5161 gcccgaagag accgctgagg ttgaattcgc tggttttgaa cttgaagaaa cctgcatcga
     5221 agccttcttc ggttggaata gtcagcttct ggaggttaac catccaaagc gtcttgtcat
     5281 cgagacgggc gtaagcccca aaaacatcga gttcaacatc ccactcttcg ggagatagaa
     5341 actgaacata tccgagagtc agctctgtgg cgttcatgga ttgcttgatc gcgaaggggt
     5401 ccatctgggt aacttttctg gaggtaatag tttcgccgag gttcaaaccc gtgaaaggcg
     5461 tttgcaattc tttgagcgag gggcctgtta tgtacaaggc tccaccctga aacctcatcc
     5521 attccaattt gggctttata agaacgtatc ccccgtaact ggtctgcttc tcctgtccga
     5581 actggccgaa gagttcgagg tctccaaagg gggtgtaaag agcggccttc gcggtgaagg
     5641 catcttccgt cgaaagccag ttcctgtacc acgaaccctg taaaagcagc caatccagtt
     5701 caagatatct caaagttatt ctggttttag catctacaat tccgctgaga tcaacatcga
     5761 acggtctcct gtcgggatcg tctagaggaa aatagtagta gggaagataa aacacaggca
     5821 gatcgccgat gtacatgacc atgttctgta ccgacatccc ctttcccggc gtaacgaccg
     5881 cgtaggacac cttgaatctg taatggggat gacctctgtc gcatgtagtt atgtaaccat
     5941 ccgtgacatt catggtggga gaggcctcaa tggtggattg aatgtaactt ccatagacat
     6001 aaaaaggaac tttagatcca tctccggagc cttctcccct caccgcgggt acctctattt
     6061 cagttctggt gtatatctcc atgctttcca gcttttcgtt ggttagatca agtatcagtc
     6121 tttctgaatt gatggtcgtt tcactgctga ccatagttac gtttccccag aaagaaatgg
     6181 atacgggact tccgttggaa gtcaagatgg aagcgttgga agcgtcgatg gtggctgtga
     6241 gagtttcact agagtaatca acccttacac cgtcctggag gagtatctca tttcccattc
     6301 ccgatcccag aatgcttccg cccactatct tcacgttcgc tccgataaga gttcccggta
     6361 cgatgatcgc cagaagtatc acggagattc tcaaaagcct cgtaagcatc tccgagagcc
     6421 tgtaactcgc tttggtgtct atgagcgaat acacaataac tccaacgatc gcgaaaacta
     6481 tattcggaat ccatggtgcc agtgtggggt tgaggaggtt ttccttgccc attccgctca
     6541 gccaggctcc cgaaccctgg taaatgacaa ccagcaagaa tgtgagtatt accgaccagg
     6601 ctttagattg aagattgaac atgagcgaca cgggcacacc cagaagaact attacgagtg
     6661 gggcaataga cattgagtac ttctcctgca acgccacttc gagcgaggat gtatctatgc
     6721 cggtgttttt gaacgccttt atgtcctgtc tcaactcggc gctcgtcttc tcttttggtg
     6781 ctttgaagct ggccaggtaa cgttctatat cctgatctat atcgaactcg gcggtattga
     6841 actgcatttc agcccccagg aaacccgaag aatctacctg gtagatcctg acatccttca
     6901 gggtccattt gttcggagag atgaccgcgg aagtggctgt tgtaagagta acttcagacc
     6961 gcgttacttc gtatattgag actcccttca attctttggt ctccggatct attcttcgaa
     7021 cgtgtatcat acgcccctga ttgtcctgca tgaagacgtt ttctttcagt gtcacctcgg
     7081 gctgtcgata aacgaacctt gccatcgctt cggttgcctt ggaagaggcg tttggaacaa
     7141 ggtagtcgtt gagtaaatag gctactccgc tgaatacgac tccgagtatc aaaaagggta
     7201 tgacgagttt cttgagggat ataccgtgag tctggagagc tatgagttcg ttgtccgaac
     7261 gcatcctgga gaggacccag aatattccca gaagcactcc cactggaacg cccataacga
     7321 tgaactccgg gagattgtag tatatgagga gaaagagctt gtctattccc accttgtatc
     7381 ggattatcat atcggaaagg taatacagaa gctccaggct gacaaacact ataaaaccac
     7441 ccagaccgat gaagaaagaa ccgagggttt gatagcctat atactttatg agtgtaccca
     7501 aaacactcgc ctcccttttc agtaggcttt gaaatcgacg ggctgaaaaa tatcgttcat
     7561 acagcgataa tactttccgg tgtcgatata gacggcggcc tccatgagct cggtgcacag
     7621 atccgcgatt tcctcgaggt cgcagaatac cctcaagaga tctacggctc tggatgtaat
     7681 cgaaggagac tgctccatgt tgctcaacag gtcgttgcga actccctcga gaagactgtc
     7741 gatgttcgaa tcgttccggc agagtctttc ggcctccaaa agagaaggat cgaccagatt
     7801 gtcgatggat cctttgagca tcagggagac tccgtcgaac atggttcgca acttttcgta
     7861 ataaccgatc ggcggcttct ttattaaaac catcgccagc tcgcatatag agacgaattt
     7921 gtccccgatt ctctcgagcg tgttcactat gctcatggtc atggtcaaaa acctcaggtc
     7981 ctttccggtc ggcgtaagga ttcccgtgag gcttattata gtcttttgca attcgatctg
     8041 gtagtggtca attacggaat cggaatagag aacatccctg gcctgatgtt cgttacgttc
     8101 aagcatcgca atagaacttt tattgcatat gttctgaatg tagcgggcca tcttgagaat
     8161 atccgaacgc aacagggc
//
LOCUS       DAOOLY010000096         8126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10384_length_8126_cov_3.922562, whole genome shotgun sequence.
ACCESSION   DAOOLY010000096 DAOOLY010000000
VERSION     DAOOLY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10384_length_8126_cov_3.922562"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLD15_06265"
     CDS             <1..226
                     /locus_tag="PLD15_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63544.1"
                     /translation="LYPLFYALVSIVRGMMTGFYPYWFLNPGAPYPSGIGSVWNLLLF
                     IIVIASLFVVAGYLLIFLKKLSSRKLKIGE"
     gene            481..1488
                     /locus_tag="PLD15_06270"
     CDS             481..1488
                     /locus_tag="PLD15_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB63545.1"
                     /translation="MKKVRVPVLLVLLVMIFMGGTTLYAKLTYGYVTPGPDTWYRKDV
                     EGFQYAASLVGVEVVVLNSDYDTEKEIANINTLVNMGVDGMCVFSFNPNGAFIAAREA
                     GMAGIPLVVTDNVGQVLQSEHDIVACIDFDWEGMGINVANHIAKNYPGEKIACIMGLF
                     EHVPVQMFRGTFEPEVEKLGVNTIVAIRDGKYTPTVAVDQAQDLIESGYDFSILFSFN
                     EEMAAAIVRMLKTRGLLNNPIKVITTNGAPYGIELIKEGSIMYSISTSPGWEGFVSFL
                     ALHAYTQGLIKEHDQQILLPNTPITPETIDDKTKVVPWDIDPVWIELTREYFPQYNSL
                     Y"
     gene            1567..3060
                     /locus_tag="PLD15_06275"
     CDS             1567..3060
                     /locus_tag="PLD15_06275"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB63546.1"
                     /translation="MERKELVKMDNLVKIYGHHRALDSVSFDLMEGEVHCLVGENGAG
                     KSTLIKILSGAISPEAGDIFIGGKTVGSLNPRQSIELGISTIYQDAELVDSLTVADNV
                     FLGDEKAGRFPLIVDSHGQEKRVGEIIDTLKMNLPVNVLVEELSASQKQMLQIVKALY
                     RDSKVLIMDEPTSSLGIDETQALMDIIRKLRQRGIGIIYISHYLEEIFEIGDRVTILK
                     DGVDMGTYNVADIKVDEVIRKMVGRDASLFYKRNSVEIGDAQLEIRDFGKTGIVEDVT
                     LSVRRGEIFGLGGLVGSGRSELVSLIFGAERPDRGEVVLNGRKLRIKSPGDAIRKGIA
                     LITEDRKKLAMLNGRDIVENSSLVHSENFKGFLLKNREERNLTNEIVNNLSVTVQDES
                     QKIEELSGGNQQKIIIGRWLIDDSLVYIFDEPTKGVDIGAKEQIYELMTGLAKRGKSI
                     IMISSDMPELLSMSDRIGVMRSGRLVEILDNAGIKEEDLIKRFIGVQ"
     gene            3080..4108
                     /locus_tag="PLD15_06280"
     CDS             3080..4108
                     /locus_tag="PLD15_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63547.1"
                     /translation="MNSVEASQGTKKVLSRRRTSILRSQWFFLLVAEIIIAVITGIVN
                     PRFFTTSNIMNILEQIAVLGIVSSGMTLLIISGEIDISVGANIGLSSCVMAMMIKSSN
                     GYLLPVIVGIALAVFNSLLVGITARAFKAPSFITSLAFTSVFQGVALAITKGSFQTIY
                     GQFEFIGTARLWQILPFSFIISIGAYIIVHFLLSYTKLGRRTYAVGSNPSAAYLSGIS
                     VNRSKLMAFLLNGIFIGIASMVLLSRIGAAQPSTGSGIELKAIGAVVIGGTPLSGGKG
                     RIIGTFFGVLLMGLISNSLNMLRVNPYFQTVTFGVLIIASLAISVLSTYRKRGKIRTS
                     SERKDEER"
     gene            4105..5037
                     /locus_tag="PLD15_06285"
     CDS             4105..5037
                     /locus_tag="PLD15_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPB63548.1"
                     /translation="MKRQNFEIENDRIRELYLKEKHRESERFTRRIDLSWSVWMFGLE
                     PIENSLERLKRNRLDFVELKGDVSMEVEKVRSALERYDMAISGVCGLFSPERDLSSPD
                     VDISKRAIEYIFGEIDFLSKFGGKYMIVVPGAVGRPEAIDSQELERSAKNLKTCAKAF
                     SARRVAAAIEPIRSAEVSIVHTTGEAIDYIASVDEPAIAHLNGDIYHMLNEERHIGRA
                     ILECGNRLVNLHIADSNRDAPGKGMIDIDTVIMASYLVGMNRPGRFLTFEPLGPYPDP
                     YVLSTQPCNVDVMEKLVSDSVSYFREREEIVRSL"
     gene            5051..5938
                     /locus_tag="PLD15_06290"
     CDS             5051..5938
                     /locus_tag="PLD15_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPB63549.1"
                     /translation="MKISFHTDAFNSAVFNFEKALQWAEKNDVHFIEPGVIDGVSWIH
                     GLGYFPHVSLAEDPMKMRKKMESYGVSLSQIDSAFPLSGNDGLYYGVPYVLKSLPWAK
                     IAGCPNIATTDGLKKPAGLSDGEALELMRRAYGTIVETAELYGININIEVHGYFTTNP
                     DLLELMLDFVDSDRLGLNLDTGNSFIAGNDPVEFCKRFIGKIRHVHIKDVSESLAAAM
                     RGKDTGIGISHSAIGDGVNAGNIKTILRLLKEHGYDGYISMECEGMGGPLIENSLAWV
                     RATLKELGIPEEKEPESCD"
     gene            6312..>8126
                     /gene="ileS"
                     /locus_tag="PLD15_06295"
     CDS             6312..>8126
                     /gene="ileS"
                     /locus_tag="PLD15_06295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPB63550.1"
                     /translation="MSGFKDVDLKRPAAKREEEVLSYWRENALPERSVNEREGYPEFV
                     FFEGPPTANGKPGIHHVISRTLKDCICRFKTMQGYQVKRKAGWDTHGLPVEIEVEKQL
                     GFSSKQEIEDYGIEKFNHKCKESVWKYESQWKKMTERIGYWIDMEHPYITLNDDYIES
                     VWWILKQYFDAGFIYEGHKIMPYCPRCGTPLASHEVAQGYRDETIDSIYVKFKLKGKD
                     NEYFLVWTTTPWTLPSNVALAVNPDFNYVKVEVTLDTGEREYYYLVRERLATLLGSEE
                     HYSIVGEMKGRELFKMEYEQLIPYGKVDKKAFYVVLGDFVSLEDGSGIVHMAPAFGED
                     DNILGKKFDLPMLQLVDREGKITKEVTPWAGMFVRDADPQIIRYLKDRDLLFKKDRLT
                     HSYPFCWRCDTPLLYYARKSWYIKTTAYKDRLIEINNTVNWYPKFVGEDRFGNWLENM
                     VDWALSRDRYWGTPLNVWKCESCGSLASVGSRQELVEKAIEEITPDIELHRPYVDDVH
                     LRCECGGTMGRTPEVIDCWFDSGAMPVAQHHYPFENREEFMEKLFPADFICEGIDQTR
                     GWFYSLMSISAFLFDQSPYKNVLVNNLVLDKSGQKMSKS"
BASE COUNT         2205 a         1872 c         2155 g         1894 t
ORIGIN      
        1 actttatccg cttttctacg cgttggtttc gatcgtcagg ggaatgatga cgggttttta
       61 tccctactgg tttctcaatc ccggagcgcc gtatccatcg gggataggat ccgtctggaa
      121 tctattgctg ttcataatag tcattgcatc gctcttcgtg gtggccggtt atctacttat
      181 ctttctcaaa aagctgtcta gcagaaagct aaagatcggt gaatgacagc gcatcttatt
      241 tttgattgcg gaaaacaatt aggagtcgga tttacgagca acggcctgag aatctcagga
      301 tattacaaat ggaggctcct gaatagccat agttggctga taagacaata gagtaccacc
      361 gggtagatga gcttatctga ctgacaagtt ggtataattc ttatgaatgc cgaaatatct
      421 cagcaatagt actgctccat cgttttgttc cctgtagatc atcaaaagga ggtaatcgct
      481 gtgaagaaag tcagagttcc cgtacttctg gtactgctgg tcatgatctt catgggtggg
      541 accacacttt atgccaaact cacctatgga tatgtgacac ccggtccgga tacgtggtac
      601 agaaaggatg tagagggctt ccagtacgcg gcgagtctgg tgggtgtaga agtcgttgta
      661 ctgaactccg actacgatac cgagaaggag atcgccaata tcaacactct ggtcaacatg
      721 ggtgttgatg gtatgtgtgt cttctctttc aatccgaacg gcgctttcat agccgccaga
      781 gaggccggca tggctggaat tccgctggtt gttaccgaca acgttgggca ggttctccag
      841 tctgagcacg acatagtggc ctgtatcgat ttcgactggg agggtatggg tatcaacgta
      901 gcgaaccaca ttgccaagaa ctacccggga gagaagatag cctgtataat ggggctcttc
      961 gaacacgtcc cggtccagat gttccgtggt acctttgaac cggaagtcga aaagcttgga
     1021 gtgaacacga ttgtggcgat aagagacgga aagtacaccc cgaccgttgc cgtcgaccag
     1081 gcccaggacc tgatagaatc cggttacgat ttctccatac tcttcagctt caacgaggag
     1141 atggccgcag caatagtcag aatgctcaag acaagaggtc tactcaacaa tccgatcaag
     1201 gtcataacca ccaacggtgc cccgtacggt atagaactga taaaggaagg cagtataatg
     1261 tactcgatct ccacctcgcc aggctgggaa gggttcgttt ccttcctcgc gctccacgca
     1321 tatacacagg gcttgataaa ggaacacgat cagcagatcc tgctgccgaa cactccgata
     1381 acgcccgaaa cgatcgatga caaaacgaag gtcgttccat gggacatcga tcccgtctgg
     1441 atcgaactga ccagagaata cttcccgcaa tacaattctc tctactgaga gtgagatgtt
     1501 atggggatgg agggacagac cttcatcccc gttttgttca tatttttgtg aggggaagag
     1561 aatatcatgg agcggaaaga actcgtcaaa atggataacc tggtcaagat atacggccat
     1621 cacagagcgc tggacagcgt ttctttcgac cttatggaag gcgaggtgca ctgtctggtc
     1681 ggtgagaatg gagccggaaa atcgactctc ataaagatcc tttcgggcgc catttctccc
     1741 gaagcagggg atatattcat cggaggaaag accgtcggct ctctcaaccc cagacagtcg
     1801 atagagcttg gtatctcaac tatatatcag gacgccgagc ttgtagattc cttaacggtg
     1861 gccgataacg tgtttcttgg tgacgaaaag gccggcagat ttccgctgat cgttgacagc
     1921 cacggacagg agaaaagagt tggggagatc atcgacaccc tgaagatgaa tctcccggtg
     1981 aatgttctgg tggaggagct atcggcttcc cagaagcaga tgctccaaat cgtcaaggcc
     2041 ctctacaggg actccaaagt gctgattatg gatgaaccga ccagttcact tgggatagat
     2101 gaaactcagg cgttgatgga cattatcaga aaactcagac agaggggtat cgggataata
     2161 tatatatccc actatctcga ggagatcttc gagataggag accgtgtaac gatcctcaag
     2221 gatggcgtag acatgggaac ctataacgtt gccgatataa aggtcgatga agtcataaga
     2281 aagatggtcg gacgcgacgc ttcgcttttc tataagagaa actccgtcga gataggagat
     2341 gcgcagcttg agattagaga ttttggcaaa accggtattg tggaagatgt taccttatct
     2401 gtgaggcgtg gtgagatctt cggactgggc ggtctggtgg gttctggccg cagtgaactg
     2461 gtatctctga tctttggtgc cgagaggccc gacagaggcg aagtggtttt gaatggaaga
     2521 aagctcagaa tcaaatcacc tggggatgcc atcagaaagg ggatcgcact gataaccgaa
     2581 gaccgcaaaa agctggctat gctcaacggc cgtgatatcg tcgaaaactc atcactggtc
     2641 cacagtgaga acttcaaggg ttttttgctt aaaaaccgtg aggagagaaa tctaacgaac
     2701 gaaatcgtca acaatctatc ggtgaccgtt caagatgaat cgcaaaagat tgaggaactc
     2761 tcgggcggaa accagcaaaa aataattata ggacgctggc tgatcgatga ctcgctcgtc
     2821 tatatcttcg atgaacccac caaaggagta gatattggtg ccaaagagca gatctacgag
     2881 ttgatgaccg gactggcaaa aagggggaag agcataatca tgatctcttc cgacatgcca
     2941 gaacttcttt cgatgagcga caggatcggt gtcatgcgca gcggccggct cgtggagata
     3001 ctcgacaacg ccggaataaa ggaagaggat cttattaagc gcttcatagg agtccaatga
     3061 ggttcgggag ggaattgcaa tgaatagtgt ggaggcttct caagggacaa aaaaagtact
     3121 ttcgagaagg aggacctcga tactgaggag ccaatggttc ttccttctag tggcggagat
     3181 aataatcgcg gtcataaccg gaatagtcaa ccccagattc tttaccacca gcaacatcat
     3241 gaacatactc gagcagattg cggttcttgg aatagtctcc tcgggcatga cgctcttgat
     3301 aatctccggg gagatagata tatcggtcgg agccaacatc ggactctctt cttgtgtgat
     3361 ggccatgatg ataaagagca gcaacggcta tctgcttccg gttatcgtgg gaatcgcgct
     3421 ggccgttttt aacagtttgc ttgtgggtat aacggcaaga gctttcaagg ctccgtcctt
     3481 tataacttct ctcgccttca ccagtgtttt ccagggtgta gccctggcaa taaccaaagg
     3541 ctctttccag actatatacg gacagttcga gttcatcgga actgcacgtc tctggcagat
     3601 ccttcctttt agtttcataa tcagtatagg ggcttatata atcgttcact tcctcctctc
     3661 gtacaccaaa ttgggcaggc ggacttacgc cgtcggaagc aacccttcgg ccgcctacct
     3721 atctggaatc tctgtgaaca ggtccaaact tatggctttc ctcttgaacg ggatctttat
     3781 agggatagcc tccatggtgc tgctttcaag aatcggtgcg gcacagcctt caacaggtag
     3841 cgggatcgag ctaaaggcga tcggagcggt cgtcatcggc ggaacgccgc tttcaggtgg
     3901 gaagggtagg ataatcggaa ccttcttcgg tgtgcttctc atgggtttaa tatccaactc
     3961 gttaaacatg ctcagggtca atccctactt tcagaccgtc accttcggtg ttctcataat
     4021 agcctcgctg gcaatcagcg tgctcagcac ttacaggaag cgcggaaaga taagaacttc
     4081 ctccgaaaga aaggatgagg agcggtgaaa agacaaaact tcgagatcga gaacgacaga
     4141 atccgcgaac tttatttgaa agaaaaacac cgggaatcgg aaaggttcac cagaagaatc
     4201 gacctatcat ggagcgtctg gatgttcggt cttgaaccca tcgagaactc tctggagagg
     4261 cttaagagaa acagactgga ctttgtggag ctcaaaggcg acgtttcgat ggaagtcgaa
     4321 aaggtgaggt cggcactcga gaggtacgac atggctattt ctggtgtctg cggtctcttc
     4381 tcgccggaga gagatctctc cagccccgat gtagatatct ccaaaagggc gatcgaatat
     4441 atattcggag agatcgactt cctctcaaag ttcggcggaa aatacatgat agtcgtaccc
     4501 ggcgccgtgg gaaggccgga agccatagat tcacaggaat tagagcggag tgcgaagaac
     4561 ctaaaaactt gcgctaaagc cttctctgcg cgtcgagtgg ctgccgccat agagcctata
     4621 cgttccgccg aggttagcat cgtccacacc acgggcgagg ccatagacta tatcgcttca
     4681 gtcgatgagc ctgccatcgc gcatcttaac ggtgacatat atcatatgct caacgaggaa
     4741 aggcacattg gccgtgccat cctcgaatgt ggcaataggc tggtgaacct ccacatcgcc
     4801 gacagcaaca gggatgcacc gggaaaggga atgatagata ttgacacggt cataatggcc
     4861 tcttatctgg taggcatgaa tagacccggt agatttctta ccttcgaacc gctcggaccg
     4921 taccccgacc cttatgtatt atccacacag ccatgtaatg tagatgttat ggagaagctg
     4981 gttagcgatt cagtgagtta cttccgtgaa agggaagaga tagtaagatc actttaaagg
     5041 gggaacagca atgaagataa gcttccacac agatgctttc aactcggcag tattcaactt
     5101 cgaaaaggct ctccaatggg cagaaaagaa cgatgtacac ttcattgaac caggagtgat
     5161 agacggtgtc tcatggattc acggtctggg ttattttcca cacgtgtcgc tcgcggaaga
     5221 cccgatgaag atgcgaaaaa agatggagag ctacggtgtt tcgctttcac aaatcgattc
     5281 ggccttcccg ctctcgggca atgatggttt gtattacggc gttccgtacg tattgaagtc
     5341 cttgccatgg gctaaaatcg ccggctgtcc caacatcgcc acgaccgacg gactgaaaaa
     5401 accggccggc ttaagcgacg gtgaagctct cgaacttatg agaagggcat acggtaccat
     5461 cgtcgagacg gccgagctct acggaatcaa cataaatata gaagtccacg gctacttcac
     5521 taccaaccct gacctcctcg aattgatgct cgatttcgtc gatagtgacc gcctgggact
     5581 aaacctggat acgggaaaca gcttcatcgc aggcaacgat cctgtggaat tctgtaaacg
     5641 tttcatcggg aagataaggc acgtacatat caaagatgta tctgagagtc tggcggctgc
     5701 tatgagagga aaggatacgg gaattgggat aagccactcg gccatcggag acggggtgaa
     5761 cgccggaaac ataaaaacta tacttcgact gctcaaagag cacggatacg acggctacat
     5821 aagcatggaa tgcgaaggca tgggcggacc tctgatagaa aactcacttg cctgggtacg
     5881 ggcgacgttg aaagagctgg gaattcccga ggagaaggag cctgagtcct gcgattgaac
     5941 gatcttgtat ttctcactag taaccatcgt gctttttcga aggatgggta cacgatttgg
     6001 gataattgac gctttttcca agtaccttgg acgactcttt tgctcttcac ggttgttgtc
     6061 ggcttgacaa agtgccaatg tattgtatta tttatacatc gagtcgagag agccaggagg
     6121 agatgtaacc gtgacggccg caaagactga accggggatt ataatctgaa ttttccttca
     6181 cagaccgcga tcatgtccgg tcttttctct cgcgcaccgc atgttacttt tcccttaatg
     6241 aaaacctcaa gagcaacgtt cataattgaa tcatcggatt ttgaaataca caaaacagga
     6301 ggagacttag tatgtcgggt tttaaagatg ttgacctgaa acgtcccgct gctaagaggg
     6361 aggaagaggt tctctcttac tggcgtgaaa atgcactccc ggagagaagc gtgaatgaga
     6421 gggagggata tccggagttc gtctttttcg aagggccgcc aacggcaaac gggaagcccg
     6481 gtattcacca cgttatttcc agaactctga aagactgcat atgtcgtttc aagactatgc
     6541 aaggatacca ggtgaagagg aaagccggct gggacactca cggtctccct gtagagatcg
     6601 aagtcgagaa gcaactcggc ttttcatcga agcaggagat tgaggactac ggcatcgaga
     6661 agttcaacca taagtgcaag gaatcggtat ggaagtacga gagccagtgg aagaagatga
     6721 ccgagcgcat tggttactgg atagatatgg aacatcccta tatcacgctc aacgatgact
     6781 acatcgaatc ggtatggtgg attctcaagc agtacttcga tgccggcttc atatacgagg
     6841 ggcacaagat aatgccctac tgccccagat gcggcacgcc tctggcctct cacgaagtgg
     6901 cgcagggcta cagggacgag acaatagatt cgatatacgt caagttcaaa ctgaagggca
     6961 aagacaacga atacttcctg gtctggacga ccactccctg gacgctgcct tccaacgttg
     7021 cgctggccgt aaaccctgat ttcaactatg taaaggtcga agttacactc gacactggcg
     7081 agagggagta ttactacctt gtaagggaga gactggccac ccttctcgga agcgaggagc
     7141 attacagtat cgttggagag atgaaaggca gagagctctt taagatggaa tacgagcagc
     7201 ttattcctta tggaaaggtc gataagaaag ccttctatgt ggtactcggc gatttcgtct
     7261 cgcttgaaga cggttccggc atcgtccata tggcgccagc cttcggcgag gacgacaata
     7321 tactgggaaa gaagttcgat ctgccgatgc tccagctggt cgatagagaa gggaagatca
     7381 caaaagaagt aacgccatgg gcggggatgt tcgtcagaga tgccgacccg cagattattc
     7441 gttacctcaa ggacagagac ctcctcttca agaaggacag gttgactcac tcctacccct
     7501 tctgctggcg ttgcgatacg ccactgcttt actacgccag aaaatcctgg tatataaaga
     7561 caactgccta caaagataga ctgatagaga taaacaacac ggttaactgg tatccaaagt
     7621 tcgtcggcga ggatcgcttc ggcaactggc tcgaaaacat ggttgactgg gcgctctcga
     7681 gagacagata ctggggaacg ccgttgaacg tatggaaatg cgaaagttgc ggctcacttg
     7741 cctcggtagg ttcaagacag gagctcgtcg agaaggctat cgaggagata acgccggaca
     7801 tagagctcca cagaccgtac gtcgatgacg tgcatcttcg ctgcgagtgt ggaggaacga
     7861 tgggcaggac tcccgaggtc atagactgct ggttcgactc cggtgcgatg ccggtcgccc
     7921 agcaccacta tcccttcgaa aacagagagg agtttatgga aaagcttttc ccggccgatt
     7981 tcatctgcga aggcatagac cagaccagag gctggttcta ctctctaatg tcgatctcgg
     8041 cctttctctt cgaccagtcg ccctacaaaa acgtgctggt taataacctg gtgctcgaca
     8101 agagcggtca gaagatgtct aaatcg
//
LOCUS       DAOOLY010000097         8120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10395_length_8120_cov_3.836082, whole genome shotgun sequence.
ACCESSION   DAOOLY010000097 DAOOLY010000000
VERSION     DAOOLY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10395_length_8120_cov_3.836082"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PLD15_06300"
     CDS             <1..1527
                     /locus_tag="PLD15_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB63551.1"
                     /translation="AMSSKEAQLRGEEAVHELSLARKRLAAIVEASPVPIISLDMEYN
                     VTEWNQAAEKVFGWKRSEVIGRPLPIVPEEDVQNHRDLTEKTRSGDYQRGTLLLRKRK
                     DGSKVVVRLHNATIFNEEGQPEGILGILEDVTRQQEAERALKESETRFRELYTNMNTG
                     VAIYKIGENDEAVFLDMNPAGCRITGVSRDEIVGRRVEEIFPGVIEMGLYDIIIQVFK
                     SGLSQRHPLKKYKDNRIAFWTDNYVYRHGKDEIVVIFEDITQREKTLEELERRVKKRT
                     AELEAVNKELESFAYSVSHDLRAPLRAIKGFAQIISERYAGVLPSEAGRYFDYIVKAG
                     ENMSILIQDLLDYSRLGRVALKIEAVSLAHTVDKALDTLCSKIDEERGKITVEGTLHF
                     VEADITLLNRVLANLIENALTYHAPERPPEINISSRREDGYVFLTIKDNGIGIDRKFH
                     NKIFEIFQRLHSADDYQGTGIGLAIVRKCVDLMGGEISLESMPGEGSVFSVKLKEALN
                     "
     gene            1529..1975
                     /locus_tag="PLD15_06305"
     CDS             1529..1975
                     /locus_tag="PLD15_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB63552.1"
                     /translation="MSAAVILLVEDNLMDVELTLDAFAQVGLKNTVTIARTGEEALNY
                     ICGKGKFADRKNFPTPDLVLLDLKLPGISGHEVLAELKNDERTKRIPVVVLTSSHEEG
                     DRAVCYDNGVNSYLVKPIDFGEFVKVVRAIGDYWLTLNVGPPEALR"
     gene            1991..3001
                     /locus_tag="PLD15_06310"
     CDS             1991..3001
                     /locus_tag="PLD15_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB63553.1"
                     /translation="MKTTRNWLLAYIALYIASLYLFPLTSRILTLSLILAMLIEVPTG
                     FFKSERARRLATLLTGVGLVVLVVYLLSSSIPMIISNISSFGNEIDRILEGERFNRLM
                     ERFPESVAGSVKDFLGSLNDMMIGFALDTARKVAAGASSWLTGTVLLVIGSVYLLRNK
                     GNFSEKIVPIVFPGCDREGLRKFLGGFNRDLQNYVFQRTLIALAVGAVIGVGAFLLGI
                     PHPLFFAILGAITNYIPYVGVVITGIPFLLVGNATHGLWGVFGVIVVLLVAGVIDGWV
                     MTPALMSNRLQINWFVILVAVIAIGELLGIYGMIIGIPALLFIRRFWSDFVMCRNDGI
                     LE"
     gene            3021..3650
                     /locus_tag="PLD15_06315"
     CDS             3021..3650
                     /locus_tag="PLD15_06315"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPB63554.1"
                     /translation="MKRYIDLTRTIVDEQAVYPGDDATSLKRSRNLSIDGFNNHRLQI
                     SMHSGTHIDGPMHMSDSPLYIDQMPLEKFVGSGVLLDVRGHSEILLREEYVDRIAERS
                     IVLFWTGRDEIFGNREYFVENPFITEDFAKLLVEKKVKAVGFDSSSPDRYPYPVHGIL
                     FSNGVLIIENLTNLASLQGVVNFEVLTLPLKIHADSSIARVIAAVQENE"
     gene            3662..4579
                     /locus_tag="PLD15_06320"
     CDS             3662..4579
                     /locus_tag="PLD15_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB63555.1"
                     /translation="MKIRKIGKRGVLFTFEDGDSPIGCDTSVYLVLGDKRAYLCDTFL
                     GPQWMDVIKLYLRDTHYEGRFVVFNSHSDFDHVWGNGAFEDSNILAHTLARKRMKERW
                     EFDYSKFSRFRRDEVSMRLPNITFNDGLFFEDDGIEFRHAPGHTVCSSVCLDWQESVL
                     FAGDLLEEPVPVTLWGDLVTFTETLKSLRDLNFSTVISAHSGVVGPELCDRNIEYIKD
                     LLLRKEVAFPAGADEGAHPFNVKYLQYLKYEELARAKEGKSFDLSKFKREFWALAGYG
                     QDRMAEESKIIKETPPERYEEAWERYLRR"
     gene            4938..7646
                     /locus_tag="PLD15_06325"
     CDS             4938..7646
                     /locus_tag="PLD15_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63556.1"
                     /translation="MRKVVLVSLVVLVVLLTGCIKIFRANEIKGTVFTDEYIENALVK
                     VLDVEGNPVVEGEFLTDEYGRFSIPVPEGLQFPVVVLATFDTGEEPEDTDALASIVEE
                     SFYTEQILVNPVSSVFAAYMFRMEISYKDAVDQVRDAFSVPPDINILSRLYPKFETTY
                     FSMERLASFVEDYFGGVYRDFVETIVEAIVGGARYNFGVAATLDFDISGLAKDLLKGI
                     TTGIISKGAISGISWVLKSLFGLGDQTTELINEILSQIQEVNARLDTMSEKLDTIDTE
                     IQSILTKLDQQYYDSYIRMVDQNYFTPIEICTGKYLYITTLPATDSTDLSIESLMQDI
                     WNSKLDQVMLNIKKVIVGGTAETSYLSLFEIYMTNAMNSLRTMGLIDEPTGDLGFYKR
                     CSPDSPAFIEYIQAYVALFKALTTRQLLALNLLVEYEHKQGTSLAPLHLQNYTAYIDE
                     EVEVFWQWLETFVTYALGGEALMTEPLYDPDKAWFFNEADNAALMALGKDSGIVVRVF
                     WNTQPMDFSVAGSTELLQYSLKTALGYLTPAYDIFKDPAKGVKLTLDKQEIQLTGVDE
                     AIQANDPNGELRTLNYTHQDVDNTLEPEILMRRYIFENLSEGRYSISRSTNSNIVQTS
                     YTHTLLENTHKNIANNLFIADEYLSESNYALNICSETSNVQSLAISAYMPSMRINNLE
                     REMTYGRSSNVADHDWVGVLNYALNRYMKISDESYWDRSWILFIPTWDSYGVDLVKVN
                     GTDREASGRYSCRLILTNKGDKGTGDGWGWQQGTVTKDHAVWMRAPSQLSDMCLAPYN
                     LSKYYSKDKKDDKYKMLSTTYCLYLNHETSQWDLIIEPTFINVLGLFFGGDKVYYGEL
                     VRLRQVSSGKYISFNKVYTDSSSYYNCGSVNDNFKYNMNNWWVFYR"
     gene            7920..>8120
                     /locus_tag="PLD15_06330"
     CDS             7920..>8120
                     /locus_tag="PLD15_06330"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPB63557.1"
                     /translation="MRKVFLVSLLLALLFCTSFGVTNLYLATGGTSGTYYPFGGVIAQ
                     VIGQRFPDIRIRVQSTGASAANI"
BASE COUNT         2119 a         1936 c         2163 g         1902 t
ORIGIN      
        1 gccatgtcca gcaaggaagc gcagctcagg ggtgaagagg cggtacatga actctctctg
       61 gccagaaaaa ggcttgccgc gatagtggaa gcttcgccgg tgccgattat cagtctggat
      121 atggaataca acgtgacaga atggaatcag gccgccgaaa aagtcttcgg ctggaaacgc
      181 tccgaagtga taggtagacc gctgcccata gttcccgagg aagatgtcca aaaccacaga
      241 gatttgactg aaaagacccg gtcgggagat taccagaggg ggacgctgct gctgagaaag
      301 agaaaggacg gctcaaaggt ggtggttcga ctgcacaacg ccacaatctt caacgaagaa
      361 ggtcagcccg aaggtattct ggggatactc gaagatgtca ccagacagca ggaggccgag
      421 cgggcgctaa aagagagcga aacgcgcttc agagagctat atacgaatat gaacaccgga
      481 gtagccattt acaagatcgg cgaaaacgac gaggcggtct tcctggacat gaatcccgcc
      541 ggatgcagga taaccggagt tagccgtgac gagatcgttg ggcggagagt cgaggaaatc
      601 tttcccggtg taatcgaaat ggggttgtac gacataataa tacaggtgtt taaaagcggt
      661 ttgtcccagc gccatcccct aaagaaatac aaagacaaca gaatcgcttt ctggacagac
      721 aactacgttt accgtcacgg gaaggatgag atagtcgtta tattcgaaga cataacccag
      781 agagaaaaaa cgctggaaga actcgagaga cgggtcaaga agagaacggc tgagctggaa
      841 gccgtcaaca aagagctcga atccttcgcc tattccgttt cgcacgacct cagggctcct
      901 ttgagggcca taaaaggctt tgcccagatc atttcggaac gttatgccgg ggtgctgcca
      961 tcggaggcag gcagatattt cgattacata gtaaaggccg gagagaacat gtcgatactg
     1021 attcaggatc ttctggatta ctcgagactc ggtagagtgg ctttgaagat cgaagcggtg
     1081 tcgcttgcac ataccgtcga caaagcgctc gataccctgt gttcgaaaat tgacgaagaa
     1141 agaggaaaga tcacggtaga gggaacgctt cacttcgtag aggccgatat cacgcttctg
     1201 aaccgagtac tggcgaatct tatagaaaac gcgctcacct accacgcacc tgaaagaccg
     1261 cccgaaataa acatatcctc caggcgagaa gacgggtacg ttttcttgac gataaaagac
     1321 aacgggatcg ggatcgacag aaagtttcac aataaaatct tcgagatctt tcagcgcctg
     1381 cattcggccg atgattacca gggtaccggt attggactgg cgatagtgag aaaatgcgtc
     1441 gatcttatgg gtggcgagat ctctctggaa tcgatgcccg gcgaggggag cgttttctcc
     1501 gtaaaattga aggaggcttt gaattgagat gagtgctgcc gttattttgc tggttgaaga
     1561 caacctgatg gacgtcgaac ttacgctgga cgccttcgct caggtcggtc tgaaaaatac
     1621 cgtaaccata gccaggacag gcgaagaagc gctgaactat atctgcggaa agggaaaatt
     1681 cgccgacaga aagaactttc ccacaccgga tcttgtcctg ctcgacctga aactgcccgg
     1741 tatttcaggg cacgaagtac tggcggaact gaagaacgat gagcgtacaa aaaggatccc
     1801 ggttgtcgtg ctaacctcct cgcacgagga aggcgacagg gccgtctgtt atgacaacgg
     1861 agtcaactct tatctcgtaa aaccgatcga tttcggtgaa ttcgtgaagg tcgtccgcgc
     1921 gatcggcgat tactggctca cactaaacgt cggaccgccg gaagccctcc gataagatta
     1981 gcggtgactc atgaagacta cacgaaactg gctgctcgcg tatatagctt tgtatatagc
     2041 ctctctttat ctctttcctc tcacctcgag gattctcacg ctctctctca tactcgccat
     2101 gttgatagag gtgccgacgg gttttttcaa aagcgaaagg gcacggcgtc tcgcgacgct
     2161 gctgaccggg gtcggtctgg tggtgctggt ggtatatctc ctttccagct cgataccgat
     2221 gataatttcg aatataagct ccttcggaaa tgaaatagac aggattctgg agggcgaaag
     2281 attcaacagg ctcatggaga gatttccgga atccgttgcc ggaagtgtca aagattttct
     2341 cggaagtttg aacgacatga tgatcggatt tgcgctggat acggccagaa aagtggcggc
     2401 cggagcctcg tcctggttga ccggtaccgt gttgctcgtt atcggctcgg tctatttatt
     2461 gagaaacaaa gggaatttct ctgagaagat agtgccgatc gtctttcccg gttgcgacag
     2521 ggagggtctg cgtaaatttc taggagggtt caaccgcgac ctgcagaact acgtctttca
     2581 aagaactctc atagccctgg cggtaggcgc ggtgataggc gtcggagctt ttctgctggg
     2641 aattcctcat ccgctctttt tcgcgatact cggagctatc accaactaca taccctacgt
     2701 gggcgtcgtt ataacgggaa tccccttcct gctggtggga aatgccacac acggactgtg
     2761 gggtgtcttc ggagtcatcg ttgtactgtt ggtcgccggt gtgatcgacg gatgggtaat
     2821 gacgcccgcg ctgatgtcga acaggctgca gatcaactgg ttcgtaatac tggtagccgt
     2881 gatcgcgata ggagagcttc ttggcattta cggtatgata ataggcatcc cagcgcttct
     2941 tttcataagg cgtttctggt cggatttcgt tatgtgccgc aacgatggta tccttgaata
     3001 atcgagaatc gaggtgaaac atgaagagat acatagatct tacgagaacg atagtcgacg
     3061 aacaggcagt ttatccggga gacgacgcga catcgctgaa acgctccagg aatctctcta
     3121 tcgacggttt caacaaccac aggctccaaa tctcgatgca ttcggggacc cacatagacg
     3181 gccccatgca catgagcgat tctcccctgt acatcgacca gatgccactc gagaagttcg
     3241 tggggagtgg tgtgttgcta gatgtgaggg gtcatagtga gatccttctg agggaggagt
     3301 acgtcgacag aatagcagaa cgtagtatag tgctcttctg gacgggcaga gacgagatct
     3361 ttggcaaccg tgagtacttt gtcgaaaacc cctttattac cgaagatttc gcgaaacttc
     3421 tggtcgagaa aaaagttaag gcagtcggct tcgactcctc ttcacccgac cggtatccct
     3481 acccggtgca cgggatactc ttctcgaacg gggtgttgat tatcgagaat ctgaccaacc
     3541 tcgcatccct gcagggagtt gtcaattttg aagtcctgac gctccctctg aagatacacg
     3601 ccgattcctc gatagcccgc gtgatcgcgg cggttcagga gaacgaatag ggggtgtcga
     3661 tttgaagata cgaaagatag gaaagagggg agtgcttttc acctttgaag acggcgactc
     3721 ccctataggc tgcgatacct ccgtctatct ggtgctggga gataagaggg cctatctctg
     3781 cgacaccttc ctcggtccgc aatggatgga tgttataaag ctatacctgc gagataccca
     3841 ctatgaaggg cgcttcgtgg tcttcaactc gcactcggat ttcgaccatg tatggggcaa
     3901 cggggctttt gaagattcaa acatactggc ccacactctc gccagaaaga ggatgaagga
     3961 gagatgggag ttcgactatt cgaagttttc gcgctttcgt cgggacgagg tctctatgag
     4021 gttgccgaac atcactttca acgacggcct tttcttcgag gacgacggaa tcgaattccg
     4081 tcacgcgccc gggcacacgg tctgctcttc ggtttgcctg gactggcagg aatcggtgct
     4141 cttcgccggc gatctgcttg aagaaccggt gccggttacg ctgtggggcg atctggtgac
     4201 cttcacggag actctcaaat ctctgaggga cctgaacttc tcgaccgtga tttcggcgca
     4261 ttcgggagtg gtgggtccgg agctgtgcga taggaacata gagtacatca aagatctgtt
     4321 gctgaggaaa gaggttgcct tcccggccgg cgccgacgaa ggcgcgcacc ccttcaacgt
     4381 caaatacctg caatacctca aatacgaaga actggccagg gcgaaagaag ggaagagctt
     4441 cgacttatcg aaattcaaaa gagagttctg ggcgctcgca gggtacggtc aggatcggat
     4501 ggcggaggaa tcgaagataa tcaaggagac cccgccggaa agatacgaag aggcctggga
     4561 gagatacctg cgcagatagt aactttcaga ccatacatag tggcacgttt atttcaatct
     4621 cgctttcatg gggtatcgac gactacggat acggcctgat gagcttcaag acgattccta
     4681 tccttcaacc ggccaggtac aattgccggg aagtatgaga tctatagagg ttctactccg
     4741 cagcagtaaa gggccgaacg ctttctgctt ctcttcacag aagatttttt ccgatagttc
     4801 gcacaggcga ctgatttctc cttttttttg tataataagt tgtcaaatca acttgtacgc
     4861 gctccatact ctttacttag ccgaatcttt caccttgaag ctgatttgcg aatcaggata
     4921 attgggggcg agttgggatg agaaaagtgg tgcttgtatc acttgttgtt ctggttgttc
     4981 tgcttacagg ctgtatcaag atcttccgtg cgaacgagat caagggaacg gtattcacgg
     5041 acgagtacat cgaaaacgcc ttggtaaaag tcctcgacgt ggaggggaac cccgtcgtcg
     5101 agggcgagtt tctcaccgac gaatacggga ggttctcaat tccagttcct gaaggcctgc
     5161 agtttcccgt agtggttctt gccaccttcg ataccggaga ggaacctgaa gataccgacg
     5221 ctctggcttc gatcgtcgaa gaatcgttct acaccgagca aattctggta aaccctgtat
     5281 cttctgtctt cgcggcgtat atgttcagaa tggagatcag ctacaaagat gctgtcgacc
     5341 aggtaaggga tgccttcagc gttcctcccg atatcaatat cctgagccgg ctttatccga
     5401 aattcgaaac tacctatttc tccatggaga gactggcgag tttcgtggag gattattttg
     5461 ggggagtgta cagggatttc gtcgaaacca tcgttgaagc gattgtcgga ggggcgaggt
     5521 acaacttcgg cgtggcggcg acgctggatt tcgatatatc cggtctggcc aaggatctct
     5581 tgaaaggcat aactacgggc ataatctcga aaggggctat cagcggcata tcgtgggttt
     5641 taaagagcct gttcgggctg ggcgaccaaa ccaccgagtt gataaacgag attctcagcc
     5701 agatacagga agtaaacgcc aggctcgaca ccatgtctga aaagctcgac actatcgaca
     5761 cagagatcca gagcatcctg acgaagctcg atcagcaata ctacgattcc tacatcagaa
     5821 tggtcgatca gaactacttc actccaatcg agatctgcac agggaaatac ttgtacatca
     5881 cgactctgcc tgcgaccgat tcgaccgatt tgagtataga atcgctcatg caggatatct
     5941 ggaacagcaa actggatcag gttatgttga atatcaagaa ggtcatcgtc ggaggaaccg
     6001 ccgaaaccag ttatttgtcc ctcttcgaga tttacatgac caatgccatg aactccttga
     6061 gaacgatggg attgatagat gagcccaccg gggatctcgg cttttacaaa agatgctctc
     6121 ccgactcccc ggcgttcata gagtacattc aggcttatgt cgcccttttc aaggcgttga
     6181 cgacccgcca gctgcttgct ttgaacttgc ttgtggaata cgagcacaaa cagggaacca
     6241 gcctcgcccc gcttcacctt caaaactaca ccgcctatat cgatgaagag gttgaagtgt
     6301 tctggcagtg gctcgagacg ttcgtaacgt acgcgctcgg cggagaagct ctcatgaccg
     6361 aaccattata cgacccggac aaagcctggt ttttcaacga agccgacaac gcggccctga
     6421 tggctctcgg aaaggattcg ggaatagtag tcagagtgtt ctggaacaca cagccgatgg
     6481 acttcagcgt tgcgggttcc acagagttac ttcaatatag tcttaaaact gccctgggtt
     6541 acctgactcc cgcttatgat attttcaagg atccggcgaa gggtgtgaag cttaccctcg
     6601 acaaacagga gattcagctc acgggtgttg atgaggccat acaggcgaac gatcccaacg
     6661 gagaactacg aaccttgaac tacacccatc aggatgtcga taacactctg gagccagaga
     6721 ttctgatgag gcgctatatc ttcgagaatc tctcggaggg aagatattcg atctccagaa
     6781 gcaccaacag caacatcgtt cagacatcct atacacacac gcttctcgaa aacacccata
     6841 aaaatatagc gaataacctc ttcatagccg acgaatacct aagcgaatcg aattacgccc
     6901 tcaatatctg ctcggagacc tccaacgtgc aatcgctagc aatatccgcc tatatgcctt
     6961 cgatgagaat aaacaatctc gagagagaga tgacctatgg cagaagcagc aatgtcgccg
     7021 accatgattg ggtaggagtc ctgaactatg cattgaatag atacatgaag atttccgacg
     7081 agtcgtactg ggatagatcg tggatccttt tcattccgac ctgggacagt tacggagtcg
     7141 atctcgtcaa ggtaaatgga acggacagag aggcatcggg aagatacagc tgccgtctga
     7201 tactcacaaa caagggcgat aagggaacgg gagacggatg gggatggcag cagggaacgg
     7261 tcaccaaaga ccacgccgtc tggatgaggg ctccatcgca gttgagcgat atgtgcctgg
     7321 ccccgtataa cctctccaag tactattcaa aagacaagaa agacgacaag tacaagatgc
     7381 tctcgactac ttactgtctg tatctgaacc acgagaccag tcagtgggat ctgatcatcg
     7441 agccgacgtt cataaacgtt ctggggttgt tcttcggtgg agacaaagtg tactatggcg
     7501 agcttgtgag gctcagacag gtcagtagcg gcaaatacat ctctttcaac aaagtatata
     7561 cagattcctc ttcatactac aattgcggct ccgtaaacga taactttaag tacaacatga
     7621 acaactggtg ggttttctac agataaacga cctcatttcc ttcaagtgat cacgcggtca
     7681 ggccagggcc cggccgcgct tttttcttag aaatttttac aggcaggcat gcagcagagc
     7741 ctttctggcg tgtaaaatcc ttgaaatgcg tgaagagttt atgctaaact atgtttaact
     7801 ccaaattccc gattattggt acgtaaaata aaatcgtctt tgtgtcaggt ttttctctgt
     7861 cgagttaagt atgcgttctg ttcgacatca agaaagatct actactggag gggatgtatg
     7921 tgagaaaggt tttcctggta agtttgcttc tcgcactgct tttctgcacc agttttgggg
     7981 taacgaatct ctatctggca acaggcggaa cctctggaac ttattacccg ttcggaggtg
     8041 tcatcgccca ggtcatcggt cagcgcttcc ctgacatcag gatcagggtt cagtcaacgg
     8101 gcgcctcggc cgccaacatc
//
LOCUS       DAOOLY010000098         8080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10459_length_8080_cov_3.955514, whole genome shotgun sequence.
ACCESSION   DAOOLY010000098 DAOOLY010000000
VERSION     DAOOLY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10459_length_8080_cov_3.955514"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(621..1865)
                     /locus_tag="PLD15_06335"
     CDS             complement(621..1865)
                     /locus_tag="PLD15_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB63558.1"
                     /translation="MKGFSELRFAGPKLVFVVFLIMFSLMFLPFHVFQSETVAYTQRV
                     YSNIYQKAVDALLTTLSIGYFQWNAMFNAFETENYEFIDSMFEEILNDISPVRSINVI
                     ERPKEFSAADDYELFVANEELYAYANVRDSLADNTTERFVVMISIDKRLVLESLSMAD
                     RISFSNKGDELFAYGLHVDIKGYDLYNWLVFHAFASGLIGVLLMMEVLAIAFSRYHHT
                     QGLQTTVYMWELQDPYTTYHSRNVAKIAEILGKRLKLRKKKLVELVNGAKLHDIGKMG
                     ISTSILRKRGPLNEIEWEVMLSHPERGKRLLEQFKYLGKYIPYAYMHHEREDGSGYPR
                     GLKGEEIPFEVKILAVADVFEALTANRPYREAYSFPQAAEMMTEMPLDQKIVSELCKA
                     LPELEKELSDRGVSGDTWVKVV"
     gene            complement(2418..2492)
                     /locus_tag="PLD15_06340"
     tRNA            complement(2418..2492)
                     /locus_tag="PLD15_06340"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2457..2459),aa:Glu,seq:ctc)
     gene            2882..4108
                     /locus_tag="PLD15_06345"
     CDS             2882..4108
                     /locus_tag="PLD15_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HPB63559.1"
                     /translation="MKMTFLPHSRLGKWGFALSISFVILISLQVRFSIIPIPVFATVS
                     LGVAGFVAAIVAFIRKDRSIGTFISIVIGAVILFVFGSILLDSTDFFKGFPVVKDYSQ
                     RTEGSDRSGDGMNLGEISQSGEHIYMVVKNRLYRVNADWTGRTKVTEFPVSSIFISGE
                     WIYFTDDPDISNLYRMKKDGSERTKLSEDNIAQYCVSDDMVLYSTKKSLVEFNEIKKK
                     AMTSVDILLETEAGTLNKMKTDGSEKTTLCKVSLEPGKIEIHAEWIYYEDYGKLYRIK
                     IDGTERTMISGKGRFGYVTDTWLYFTALRDTGTDAYYDLDVVRMKIDGNERSTLASLD
                     RVYFFTFDEGWFYFVLKSEKGLHRMRPDGTEMQKLNNINIWALDGVSGNWMYISDYSG
                     AYYRVKLDGSVGTRIN"
     gene            4160..4951
                     /locus_tag="PLD15_06350"
     CDS             4160..4951
                     /locus_tag="PLD15_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPB63560.1"
                     /translation="MFIRKWTASDAKANLIICHGIGEHSGRYDAFASFLTSRGFNVFS
                     TDLPGHGLHQGQRGYIDSFDDMKNAVVDLATAVRKGKRKLPLFLFGHSMGGLIGSRVI
                     EENPGIFRAAALSSPHLYSAKESVKKLLPVVKLVKKLAPKITFSSSSRFEPSDLSHNK
                     LAVQRYIDDPYVHDRVSPNLFLGMEESIEMVFSEVEKITIPIMIVIGSDDRVIDPSGA
                     REFYEKLGSEKRFLEIAGGMHEMFADEERKGILFEGVVSFFSEFL"
     gene            complement(4937..5824)
                     /gene="ribF"
                     /locus_tag="PLD15_06355"
     CDS             complement(4937..5824)
                     /gene="ribF"
                     /locus_tag="PLD15_06355"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPB63561.1"
                     /translation="MYVTCIGNFDGVHLGHRQIMKRTVELSRRLGVRSSAISMIYPWA
                     YYFPNFPGIIYPVSQRIELILSTGIHEVITANMAEIKHLEPREFINGLIGQGVTGIVV
                     GRDFTFGSGAKGNTALLEGMAGEMGFFVDTVPDCVLDGRRISSSWIREALAKGEIRQV
                     NALLGQRYSIRGRVYRDQQLGSKMGFPTANITRGEERLVTPKSGVYIVKSIIEGKELF
                     GLLNVGFRPTVQVTEEVKYEVYYFDFSGDLYDKNIEIEFLEFIRPELKFDGLQLLIEQ
                     IKHDEKIARRWLELHSEEL"
     gene            complement(5826..6767)
                     /gene="truB"
                     /locus_tag="PLD15_06360"
     CDS             complement(5826..6767)
                     /gene="truB"
                     /locus_tag="PLD15_06360"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPB63562.1"
                     /translation="MKSKMQKTTDGIILVDKPCDMTSHDVVNVLRRKLQTKRVGHSGT
                     LDPFASGLIIAGIGKGTRVLEYFLNMDKTYRAELELGRITDTFDLTGKIVEEREVPAL
                     SLEEVTGVLKAFIGEQLQTPPAYSAKKHNGERLYKLAREGNIINLPPSRVQIYSMEEI
                     SFDGRKISFTTRVSKGTYIRSLVMDIGYRFGCGATTTYLRRTAQGPFSLNSSSRLEEV
                     SALSIITLEEAIYFMPGLLLNEQESQRVLLGGQVHARNVVGILGSFVKDEIIRILGND
                     ERLLAVARSERTSSFVKTLQREESWERVAKLEKVLGA"
     gene            complement(6739..7110)
                     /gene="rbfA"
                     /locus_tag="PLD15_06365"
     CDS             complement(6739..7110)
                     /gene="rbfA"
                     /locus_tag="PLD15_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPB63563.1"
                     /translation="MSSSHRREMLESEIKKVLTEALSSYASHDPSVGMVSIVRVELSK
                     DKRYAVIYISAMGEEKKKKTLVERLNEDKGIFRTAIAKNIRLFKAPEIRFKEDIGIEA
                     TLRVAQLLQQIENEKQDAEDN"
     gene            7261..7950
                     /locus_tag="PLD15_06370"
     CDS             7261..7950
                     /locus_tag="PLD15_06370"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPB63564.1"
                     /translation="MNRKIGAILVLLSLASLLTAFTICIDPGHQEKANLSQEQIAPGS
                     SETKAKVSTGTRGVNTGIPEYVFNLELSFILRDRLQEENFDVVMTRERHDVDVSNIER
                     AELANSAAADLCIRVHANYSSDPSLKGMMLLIPSPTATYTAPIYEESRRASEKIMASL
                     QQIEGITSLGIRVREDMTGFNWSKVPVLIFEAGFMSNPEEDALLASQDYREALVEAIV
                     SGVVDYFLSRR"
BASE COUNT         2063 a         1940 c         1906 g         2171 t
ORIGIN      
        1 tcgaacattc ccaactacaa cgatctgatg caaaaaggat tcgaagcgta cctgaactga
       61 cgagataaaa gccgtcagtg cgggcgtttc aacaacgaaa acaaaccgga ggatcgtgtg
      121 caagttgtcc tccggttttt gtaatctgtc gttgcgaaaa gtcgttctcc accaaccgtc
      181 cgccgttgta tgcagacctc cggaagcgtt acaagtggtc cttgaaaaga ggggagagca
      241 tcatgagcct ttctcctgct gttcctatcg accgacggtt ctttgttaat tcaaatgtat
      301 tagttgagac ttgagtttcc acaacgattt gtagtatcat tactcattag ctcgcgaggg
      361 agttgtctgg cgaatcggtt atacgagtgt ttttgaaggc caatttcacc taaatcctcc
      421 ttatagttag tgattaatgc agtagatacc tccatgattc attttttccc tgttttttat
      481 cgaattccct cgcgtttctt ctctttcttc tcggctttat ataagtatta tcccgtcttt
      541 ggcatctatc aatgcccatc attatttgta tgtgaatcgt taatcagatt caaaaaacgt
      601 acggcattga gaaagctact ctaaacaact tttacccagg tgtctccgct aactcctcta
      661 tcactcaatt ccttttccag ttccggtagc gccttgcaca gttcggacac tatcttctgg
      721 tccagcggca tctctgtcat catctcggcc gcttggggga aggaataggc ctccctgtat
      781 ggtctgttgg ccgtgagagc ctcgaataca tcggcaaccg cgagaatctt cacttcaaag
      841 ggaatctctt cccctttcag tccccggggg tatcctgaac cgtcttctct ttcatggtgc
      901 atgtatgcgt aaggtatgta ttttccaaga tacttgaact gctcgagcaa tctcttcccg
      961 cgctcagggt ggctcaacat tacttcccat tctatttcgt tcaaaggacc gcgctttctg
     1021 agaatgcttg tcgagatccc catctttcct atatcgtgaa gcttggcacc gtttacaagc
     1081 tcgacaagct tctttttcct gagcttaagt ctctttccaa ggatctccgc tatctttgcc
     1141 acattccttg aatggtaagt ggtgtatgga tcctgtagct cccacatata gaccgtagtc
     1201 tgcagaccct gagtatgatg atacctggaa aacgcgatcg cgagaacttc catcatgagg
     1261 aggactccaa ttaatccgga tgcaaaggcg tgaaacacca gccagttgta taggtcgtaa
     1321 ccctttatat cgacatgaag accatatgcg aataattcgt cccctttatt tgaaaagctt
     1381 attctgtcgg ccatggaaag gctttcaaga actagacgtt tgtctattga aatcattacc
     1441 acaaatcttt cagtagtatt gtccgccagg ctgtccctca cgttcgcata ggcataaagc
     1501 tcttcatttg ccacgaataa ctcgtagtca tcagcggccg agaattcctt cggtctctca
     1561 attacattta tggatctcac aggagaaatg tcgttgagta tttcttcaaa catactgtcg
     1621 atgaactcat agttttcagt ttcaaatgca ttgaacatag cattccactg aaaataaccg
     1681 atggatagag tcgttagaag agcatcaacg gctttctggt agatgtttga gtaaaccctt
     1741 tgggtgtatg caactgtttc ggactggaaa acatgaaacg gcaagaacat gagcgagaac
     1801 ataatgagaa agacaacgaa gactaatttg ggtccggcga acctcagttc cgaaaatcct
     1861 ttcataaaac tcctcctaat cccacactcc cagatcactt ctgtttcatt ctattggtta
     1921 aatcaaaaat attatactaa ttactatatc atacgaatat tcaattctag cgttgcctgt
     1981 aagatccata gcctcatctg gattgtaaaa agtaacttat caaggaatag ataatttgtt
     2041 tttttgcgta acatactgca gaaagatttt gcatcgcttt cttcttggag gaataggcaa
     2101 tgcgatagag gttagagaca acaacccacc cagctaagtc aagaatgtcc agtaggagac
     2161 aaacccgaaa gctgatacac ttcatcgaca ggagcgtgcc gccacctctc tcagggggac
     2221 tcaagatcgg aagcatcagg agctgaccag ataggagact ggccgcacga attggtactg
     2281 gcaaagtcaa aatcttcgcc aattgcatat ggctgccgca gtcgactgga tattgcttta
     2341 agtcttctac ataatgaaaa atctcccttt cgggagatcc ttcacgggaa tttgagtaca
     2401 tcacaaggtt tcgacagtgg cagccccacg gggaatcgaa ccccgacctt cggactgaga
     2461 atccggtgga ctagccgtta tcctatgggg ccagatgaaa ataataatat catgggctct
     2521 ggcttttgta aagcacccga tttgcctcat aatagatcta tcataaatga taagtggatt
     2581 tccgtctcga ccaacgcaaa aatatgtgca aagagcgaat cgttgatttt gcccgactca
     2641 ctgaatagag atcaaatacg tcagcaactg caaacattcg gagattaata taggtccaga
     2701 ttctctcact tcgataccca acggtagcag aaagagttag tattactatg agagcgaaaa
     2761 agctacgagt atcaccgtaa attagcgccg ggattgatct atgttatcaa catcgaacca
     2821 ccgtgctgcc tatttggttt gctagacctc acggtgactg ctggcaaagg agatggcggg
     2881 tatgaagatg acttttctgc cccattcgcg gttgggcaag tggggttttg ctctgagtat
     2941 ttctttcgtg attctgattt ctctccaggt aagattctcg ataataccga tcccggtctt
     3001 tgcaacggta tctcttggag ttgcgggatt tgtagcggct attgtagctt tcataaggaa
     3061 ggacaggtcg atcgggacat tcatttctat cgtaatcgga gcggttattc tcttcgtgtt
     3121 tgggtctata ttgcttgact caacagattt tttcaaaggg tttccagtag taaaagacta
     3181 ctcgcaacga actgaaggaa gcgacaggtc gggggacggg atgaatctgg gagagatttc
     3241 ccaatcggga gaacatatat atatggtcgt caagaataga ctgtatagag taaatgccga
     3301 ctggacagga cggacaaaag taaccgagtt cccggtcagc tccatattca tttcaggcga
     3361 atggatatac ttcactgacg accctgatat aagcaatctg tacagaatga aaaaggacgg
     3421 aagcgaacgg actaagttga gcgaagacaa catagcgcag tactgtgttt cagacgacat
     3481 ggtcctctac agcacaaaga agagtctggt cgagttcaac gagatcaaga agaaagctat
     3541 gacgtctgta gatattttat tggaaaccga agcgggaaca ctcaataaaa tgaagaccga
     3601 cggatcggaa aaaacaaccc tttgcaaagt ctcgctagag ccggggaaaa tagagattca
     3661 cgctgaatgg atatactacg aagattatgg caaactttat agaataaaga tcgacggaac
     3721 cgaacgtacc atgatctccg gaaagggccg gttcggatac gttaccgata catggctcta
     3781 cttcacagcc ctaagagaca ctggaacaga cgcttattac gatctcgacg ttgtcagaat
     3841 gaaaattgac ggcaacgaaa gaagcactct tgcctcgctg gacagagtct acttttttac
     3901 tttcgacgaa ggctggttct atttcgtttt aaaatcagag aagggacttc acagaatgag
     3961 accggatgga actgagatgc agaaactgaa caacataaat atctgggctc tcgacggcgt
     4021 atctggtaat tggatgtata tctccgacta ttcaggagca tactaccgcg tgaaactcga
     4081 tggctcggtg ggaaccagga ttaattgaga tgaagagtac cctccgtaga tatataatct
     4141 acttcgaggg ggtgtttttt tgtttatcag aaagtggacc gcttcagatg caaaggccaa
     4201 tttgataata tgtcacggga taggcgaaca ttccggcagg tacgacgctt tcgcctcttt
     4261 cctgacatcc aggggcttca acgtcttttc gacggatctt cccggccacg ggctgcacca
     4321 ggggcagcga ggctacatag attccttcga tgatatgaaa aatgctgtgg tagatctggc
     4381 gacggccgtg agaaagggaa agcgcaaatt gcctcttttc ctcttcggac acagcatggg
     4441 cggtcttatc ggttcgaggg tgatcgagga aaaccccggc atcttcagag ctgccgcttt
     4501 gagttcgcct catctctact ccgcgaaaga atctgtgaaa aagcttcttc cagtagtcaa
     4561 gctcgttaag aaactggcgc cgaaaattac gttcagcagc tcatccaggt tcgaaccttc
     4621 cgatctctct cacaacaagc ttgcagtgca gcgatacata gacgatcctt acgttcacga
     4681 tagggtctct cccaatcttt ttctcggaat ggaggagagt atagagatgg tcttctcgga
     4741 agtggaaaag atcacgatac cgattatgat agttatcgga agcgacgaca gggttataga
     4801 cccctccggt gccagggagt tctacgaaaa attgggatcg gagaagaggt ttctcgaaat
     4861 cgccggagga atgcacgaaa tgttcgcaga tgaagagaga aagggcattc tgttcgaggg
     4921 agttgtctct ttcttctcag agttcctctg agtggagctc aagccagcgc cgcgcgatct
     4981 tttcgtcgtg ctttatctgt tctatgagca actgtaggcc gtcgaacttc aattcgggtc
     5041 ttatgaattc cagaaactct atttcgatgt ttttgtcgta taagtctccg gagaaatcga
     5101 agtaatagac ttcgtatttc acctcttcgg tcacttgcac ggtcggcctg aaccctacgt
     5161 ttagcaggcc gaagagttct ttgccttcta tgatcgattt tacgatgtaa acccccgatt
     5221 tcggagttac caggcgttcc tccccacgcg tgatgttggc cgtgggaaag cccatcttag
     5281 agccgagctg ctggtcacga tagactcttc cccttatcga gtatctctgg ccgagcagcg
     5341 cgttgacctg acgtatttca cctttcgcca gggcttcgcg tatccagctg ctgcttatcc
     5401 ttcgaccatc cagcacacaa tcgggaacag tatctacgaa aaagcccatc tcgccggcca
     5461 tcccttcaag cagcgcagtg tttcccttcg caccgcttcc gaaggtgaag tctctgccca
     5521 caacgatgcc ggtcactccc tgcccgatca ggccgttgat gaattcgcgg ggttccaggt
     5581 gctttatctc ggccatattg gccgtgatca cttcatggat cccggtcgag agaatcaatt
     5641 cgatcctctg agataccggg tagattatgc ccggaaagtt ggggaagtag taggcccagg
     5701 ggtaaatcat ggaaatagcc gaacttctga caccgagtct tcttgacagc tcgaccgttc
     5761 ttttcattat ttgacggtga ccgagatgaa cgccgtcgaa gtttccgatg caggtcacat
     5821 acatgttatg ccccgagtac cttttcgagt ttggcaaccc tttcccatga ctcttctctc
     5881 tggagtgtct tcacgaagga tgacgttctt tccgacctgg caacggcgag taatctctcg
     5941 tcatttccca gtatccggat aatctcgtct ttgacgaagc ttccaagtat tcctacgacg
     6001 tttctggcgt ggacctgacc gccgagaagc accctctggc tttcctgctc gttgaggaga
     6061 agcccgggca taaagtagat ggcctcttcc agagtgatta tggacagagc ggagacctct
     6121 tcgagccggc ttgacgagtt caacgagaaa ggtccctggg ctgtcctcct gagataagtg
     6181 gttgtggcgc cgcaaccaaa gcggtatcca atatccataa ccagagatct tatgtatgtc
     6241 cccttcgaga ccctcgttgt gaaggagatc ttccgtccat cgaaggagat ctcttccatc
     6301 gaatatatct gtacgcgact gggcgggagg ttgattatat tgccctccct ggcgagcttg
     6361 tacaggcgtt ctccgttgtg tttttttgcc gagtaagccg gcggcgtctg caactgctcg
     6421 ccgatgaaag ctttaagaac cccggttact tcttcaagtg aaagggccgg tacctctctc
     6481 tcttcaacga ttttacccgt gagatcgaaa gtgtcggtta tcctgcctag ttccagctcg
     6541 gctctgtagg ttttgtccat gttgaggaag tattccagca ccctcgttcc cttaccaatg
     6601 cccgctatga taagtccgct ggcaaatgga tcgagtgtgc ccgaatgacc taccctcttc
     6661 gtctgaagtt tcctgcgaag cacatttacg acatcgtggg atgtcatgtc acacggtttg
     6721 tctacgagta ttattccgtc agttgtcttc tgcatcttgc ttttcattct ctatctgctg
     6781 aagcagctgg gccaccctga gagtggcctc tatgccgata tcctctttga aacgtatctc
     6841 gggtgctttg aacaacctta tatttttggc tatcgccgtt ctgaaaatac ccttgtcttc
     6901 gttcaacctt tcaacgagag tctttttctt cttttcttca cccatagcgc ttatgtagat
     6961 caccgcatat ctcttatctt tactcagctc gactctgact atcgaaacca tgccgaccga
     7021 agggtcgtga ctggcgtaag atgaaagagc ctccgtgaga actttcttga tctccgattc
     7081 cagcatctct ctcctgtggc tgctcgacat agctctcacc tcctgaccaa ttataaccca
     7141 ccattgcggg agtttgtgga tgaatagctt cgagccttga aagtgtattg aatgccgccg
     7201 gatgctcctc gtcgaaagac tatataatag tagtaactta tagcgaacgg agtggatgtg
     7261 ttgaatagaa agataggtgc gattctagtt ctgctgtcgc tggcctcgct tttgacggct
     7321 ttcacaatat gtatagaccc gggtcaccag gagaaggcca atctctcgca agagcagata
     7381 gcgcccggct cgagcgagac caaggcaaag gtctcgaccg gaaccagagg cgtcaacacc
     7441 gggatacccg agtatgtctt caatctggag ctctctttca ttctgaggga caggttgcag
     7501 gaagagaact tcgacgtagt tatgaccagg gagagacacg atgtcgatgt aagcaacata
     7561 gaaagggcgg agctcgccaa ctccgcggca gccgatctgt gtataagggt tcacgccaac
     7621 tacagttcgg atccttcgct gaagggaatg atgcttctga taccttctcc gacggcgacc
     7681 tacacagccc ccatttacga agagagcagg agggcctccg agaagatcat ggccagtctc
     7741 cagcagatag aggggataac ctcgctcgga ataagggtaa gagaagatat gaccggtttc
     7801 aactggtcaa aggtccccgt tctaatcttt gaggccggtt tcatgtccaa tcccgaggaa
     7861 gacgcgttgc tggccagtca agattaccgc gaggcactgg tcgaggctat cgtctcaggg
     7921 gttgtggact actttctgag tcgtcgatga ccgatccatg gttcaaacga tagacgagag
     7981 atcggtcagg aagtctctga tctcgctgct ggccgattcg aagaactccc ggggatacgt
     8041 gtccttcagt attgagccat ctttcatgaa caaaaccctg
//
LOCUS       DAOOLY010000099         8073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10470_length_8073_cov_4.400474, whole genome shotgun sequence.
ACCESSION   DAOOLY010000099 DAOOLY010000000
VERSION     DAOOLY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10470_length_8073_cov_4.400474"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..641)
                     /locus_tag="PLD15_06375"
     CDS             complement(171..641)
                     /locus_tag="PLD15_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB63565.1"
                     /translation="MVLLEESSKIEEMLTLIKRIMSLIKQSFESDLKKMDVTESQILV
                     MRVLNHYGDMKVSDISRKLDLSNSTVSGIIDRLVEKKIVERKRSEKDRRIVMISLAEE
                     YQQPVKKHLIAFAQKMRRALSTATEEDLDFILEGLEKLRTILEESHNPTERGQN"
     gene            1785..2819
                     /locus_tag="PLD15_06380"
     CDS             1785..2819
                     /locus_tag="PLD15_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB63566.1"
                     /translation="MACAYADKESYFDSVSEDLGVDFKPGEELVRFLRFLRDDSNWPI
                     KVAVNVFSEIKDSVIFFCDMPPSSGKIVTVEETMESLKNDFRGKARCVVESILLNNLN
                     FSKEEVERALGGDLNIVAEAVDRASDLSENTTWFITQLVNFPQQSQEILMFALTVLKR
                     YYEDSGLRKKNLGCINETMESFGQEEYKEVASGFLELYGVTARDESPLYLLLQNTLQY
                     PGVRYAYACDKQFVVLNNQLKQLEEIVNPYITDHILRLFLKNFSDPTKMQIMVAVSQE
                     PRYVDELAKLLGLSKATISYHLSSLGELALVTGRKDRRRIYFSLNRKRIESIIKRLEA
                     FYEESDDSWK"
     gene            2816..3880
                     /locus_tag="PLD15_06385"
     CDS             2816..3880
                     /locus_tag="PLD15_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB63567.1"
                     /translation="MIAGYFEIYDLTMAIHFSLNTGPVEKVLKTLGVDYEPTKELIDF
                     RDTLLQDGEWTTRMYTTFMNELGVMAPILFPIKQKMLDGMIVTIEESLNISDEGLQMI
                     RERFIQVFADRRLKVSHETLIKMLLEQPSEVARAIDTIEGISTDTIWFINQLFFFPRT
                     AMDFLVANTLKILKLYEKSGLRELNLKTIRNYLENQSSQKIKDAFSNYLDYYDITPDE
                     SKPLYIALQNSVSHTNSGLMTYPTFHLLIMGLEEVTQDFSGRIPREVRLQSLLKMLSD
                     ETRFRILKRLNTQPVPQKRLVEFTGLAKSTISYHMGLLFKSSLIDIDPFSNIVSVRKE
                     TLRKAAADIRNLLAIKEKKL"
     gene            3877..4542
                     /locus_tag="PLD15_06390"
     CDS             3877..4542
                     /locus_tag="PLD15_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB63568.1"
                     /translation="MIKAVIFDMDGVMIDSERLYREACAEIVGRYGGAITPELFEKQM
                     GLKMEETQRVVVEIANLPLTPQEFGRMYIERFLEIARERLGPNPGLVELLEFLTPRVK
                     LGIASSTEKGVIMEIVEQIGVGRYFQAIVGGDEVSRSKPDPAIYLHAAGLLGCDPEEC
                     IVIEDSPNGIKSGTAAGMEVLAVLHDENRHLDLSGAGRVFENLIEVGEYLRRSDGLSS
                     RRR"
     gene            complement(4539..5276)
                     /locus_tag="PLD15_06395"
     CDS             complement(4539..5276)
                     /locus_tag="PLD15_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63569.1"
                     /translation="MLDIRKLEEISMNAWPGVYVRFYDGWVMRCAGGYTNRANSILPL
                     YESREDLMRKINEAERFCSSRGLQTIFKMTSISLPSELDRALESQGYVERDRAIVLTK
                     ELSGATRDLSDLDIFTTPSREWLGSFTRLSKKAAENEHWLGRILDSIVPDAYYLILKK
                     SEEAVGCAMAVREDEYFGLFGITIGQEYRRMGYGKELTEKLMALGGREGARVAYLQVD
                     IVNDVAIKLYSSLSFEEAYQYWYRVKV"
     gene            5393..6373
                     /locus_tag="PLD15_06400"
     CDS             5393..6373
                     /locus_tag="PLD15_06400"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPB63570.1"
                     /translation="MEEKRLDQNLYSTYLSRINSVLDYIEQHLAEPLTLEELSDVANF
                     SRFHFHRIFSVFMGETLSHFIWRLRLEKAAVLLANDSKKTVSEIALDCGFSSPSSFSR
                     SFKKYFELTPLEWRKEKLAQSNLDQEEGNFDHTQSNERKASFPAPIYNRSVEIMMRRT
                     NMEKMGKSVTIKQLPETDVAYVRYIGPYAGDEKLFETLFNKLCAWAGPRGLLQRSDAQ
                     YLIIYHDNPDVTEEDKLRLSVCVTVPKNTRVEGEIGRMTIAAGEYAAARFELDSSEYG
                     KAWDWLYGQWLPQSGYLPDDRLPFELYPTDVKSECEDKKVVDIYLPVKKI"
     gene            complement(6406..6756)
                     /locus_tag="PLD15_06405"
     CDS             complement(6406..6756)
                     /locus_tag="PLD15_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB63571.1"
                     /translation="MSTFSPKTFLASPVWNWGVFYEKVVREVMDKNWKSYQYWGGIKD
                     AIVDISMSPLVPQTISDNVRAEREKIISNDLHPFEGPLYDQSGKMRCSAGERPSDDEL
                     LSMNWFVRNVVGEP"
     gene            complement(6788..7228)
                     /locus_tag="PLD15_06410"
     CDS             complement(6788..7228)
                     /locus_tag="PLD15_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB63572.1"
                     /translation="MKVRLVSLLILLFAASIQAAPLRVGFVYVGSANDGGWSQKHDEG
                     RLYLEKVFGREIETGYAENVVEGEQDLEIMRSFAGHGFDIVFSTSFGFMDDVVEIAAH
                     YPETVFMHCSGYKTSENLGTYFGRICEPGYLTGLIAGGRSGGRR"
     gene            complement(7756..>8073)
                     /locus_tag="PLD15_06415"
     CDS             complement(7756..>8073)
                     /locus_tag="PLD15_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63573.1"
                     /translation="ARAVMNELYFDGGLKRYKKDTYYGGGSWILLSAYLAGHLKSNNE
                     RDISEEIKAWIERKADSKGFLPEQVPENLLEERMYAPWVEKWGEIASPLLWSHAAYID
                     MLL"
BASE COUNT         2068 a         2017 c         1928 g         2060 t
ORIGIN      
        1 tatagcctcg ggtatggcgt cttcgatacc gccctgttga ataccgacgt ttacgatatt
       61 cgacatataa tctgggaggg cgagctcgga catggcctgg acgaagagca gcgctaccgc
      121 gactatgatg aacacagtgt agggtttaag gtacctgatc agcttgaaca tcagttttgt
      181 cctctttctg ttggattgtg tgattcttct agtattgttc taagcttttc aagcccttcc
      241 agaatgaaat caagatcttc ttcagtggcg gtcgacagtg cccttctcat cttctgggca
      301 aaggcaatga ggtgtttctt gacgggttgt tgatactctt ccgcaaggct tatcataacg
      361 attcgcctgt ctttttcgct tctcttcctt tcaactatct tcttttcgac gagtctgtcg
      421 atgatccctg acaccgtgct atttgaaaga tcgagtttcc tgcttatatc gcttaccttc
      481 atatcgccat aatgattcag cactctcata acgaggatct gtgattcggt cacgtccatc
      541 ttcttaaggt cgctctcaaa actctgcttg ataagcgaca ttatcctctt gatcagtgtc
      601 aacatttctt ctattttcga gctctcttcc aacaaaacca ccttcttcgc acattattag
      661 acctttcgta gccggataat atgaattcga atatttcgca cacgaaatat attgtacagc
      721 aataagccga tagtgtcgat ggaaaaatca aaaaccggaa ctccatataa gaaaactacg
      781 gaatggcagt tcgggagcat tcgcgatgca acacgaaggg gtgtttcagg acagattgtt
      841 ccttcactca gtattttaga aaggatgtgt attgcactcg atgtttgata ttcatcgata
      901 cttaataatt atagtttgac aactatcaaa ccaggtgtta tgatctaact gtaagagata
      961 catcgaacta aggggaggga gcatgaatga aatcaacgaa agtcagatca gtgaaggaaa
     1021 ttttcgacca gatagaaaga gtcaaggtag acgccctcaa gttcggagcc ttctaccgct
     1081 tctaagaagc agaaagagaa cataacagag ccccttcaac atagaaagtc attggagtcc
     1141 ctaaaaggga ctcgtttttt tatagccgag catgctgtca acatcaaagc aaaaggcaat
     1201 ataaaaaaag aagggtctcg ctggagaccc aatgtcatct tttctttccg tgggggaaaa
     1261 aggggggttg ctttgtatgt tcggttctac tggtgtgcct tttaattaga gagccagaaa
     1321 attacccagt cttgcagaat ccgatctcgc tttgtctatg gctttctgaa tatctctaat
     1381 tcctgtgaaa gtcctttttc tcatatcaat cgctcccgca agttcgatat ttttcttaca
     1441 taatgatcat aacacctggt ttgatagttg tcaaactata attattaagt atcgatgaat
     1501 atcaaacaat actggtatga agatcattcg gcttccatta tatgttgact ctaaagacct
     1561 cgggccaaag aacggattat gaacatactt ttttggcgaa tatactgctc tattgtcggc
     1621 attcatggat gatcatatgc cggctgtttg cttattctgc atcttgagga attggttcta
     1681 agcatatcga acaccttgtg tggtataatt ctattcatat cgaacctgga gtgtggcttt
     1741 acttgcgaat ggttcccgga tatgttgaat tagttgaaat tctcatggcg tgcgcttacg
     1801 ccgacaaaga gagttatttc gattcagttt cggaggatct gggcgtcgac tttaagccgg
     1861 gtgaagaact ggtacgtttt ctccggttct tgagagacga ttcgaactgg ccgataaagg
     1921 tggccgtgaa cgtattttcc gaaataaagg atagcgtgat cttcttctgc gatatgcctc
     1981 cctcttctgg aaagatagtt actgtcgaag agactatgga aagcctcaag aacgatttca
     2041 gaggcaaggc cagatgcgtg gtcgagagta tccttctgaa caacttgaac ttctcgaagg
     2101 aagaggtcga aagggcgctg gggggcgatc taaacatagt agccgaagct gtcgataggg
     2161 ctagcgacct ttctgagaac acgacctggt tcattacgca gctggtgaac ttcccgcaac
     2221 agtcgcagga gattctcatg ttcgccctga cggttctgaa gaggtattac gaagattctg
     2281 ggctcagaaa gaaaaacctg ggctgtataa acgagaccat ggaatccttc ggtcaggagg
     2341 aatacaagga ggtcgcttcc ggctttctgg agctctacgg agtgacggcc agggacgagt
     2401 ctcctctgta tctccttctg cagaatacgc tccagtatcc gggcgtgaga tacgcctacg
     2461 cctgcgacaa gcagttcgtc gttctcaaca accagctcaa gcaactcgaa gaaatcgtca
     2521 acccctatat caccgaccac attctcagac tctttctcaa gaatttctcc gatccaacga
     2581 aaatgcagat catggtggcc gtttctcaag aacccaggta tgtagacgag ctggccaaac
     2641 ttttaggcct ttccaaagcg actatctctt accacctctc ttcactggga gaactggcgc
     2701 tggtcaccgg cagaaaggac cgcaggagga tatatttctc gctcaaccgc aaacggatcg
     2761 agagcattat caagaggctg gaagcctttt atgaagagag tgatgactct tggaaatgat
     2821 agcgggatac ttcgagatct acgatctcac gatggccata cacttctctc tcaacaccgg
     2881 cccggtcgaa aaagttctaa agacactcgg tgtcgattac gagccgacca aagaactgat
     2941 cgacttccgt gatacgcttc tccaggacgg tgaatggacg acgaggatgt acaccacctt
     3001 catgaacgaa ctgggtgtca tggctccgat actctttcca ataaagcaga agatgctcga
     3061 cgggatgata gtcacgatag aggagagcct gaatatatcc gacgagggcc tccagatgat
     3121 cagggaaaga tttatacagg ttttcgccga ccggcggcta aaggtttctc acgagactct
     3181 catcaagatg cttctcgagc aaccctccga ggtggcgcgg gccatagata ccatagaggg
     3241 aatcagcacc gataccatat ggttcataaa ccagctgttc ttcttcccca ggaccgccat
     3301 ggactttctg gtggccaaca ctctcaagat actgaaactg tacgaaaaga gcggcttgcg
     3361 cgaactcaat ctcaaaacca taaggaacta tctcgaaaac cagtcctctc agaagataaa
     3421 ggatgccttt tcaaactatc tcgattacta cgatataact cccgatgaga gcaaaccgct
     3481 ttatatagcg cttcagaact cggtctctca caccaattct ggcctgatga cctatcctac
     3541 cttccatctc ttgataatgg ggctggaaga ggtgactcag gacttctccg gaagaattcc
     3601 cagagaagtg cgtcttcaga gcctgttgaa gatgctctcc gacgaaaccc gtttcaggat
     3661 actcaaaaga ctcaacactc aacccgtacc gcagaagcgc ctcgtagagt tcaccggcct
     3721 ggcaaaatcg accatctctt atcatatggg gctgcttttc aagtcttcgc tcatagacat
     3781 agatcccttc agcaatatag tctccgtcag gaaggagact ttgagaaagg cggcggccga
     3841 tatcaggaac cttctggcga taaaggagaa aaagttgtga tcaaagctgt gattttcgat
     3901 atggacggag tgatgataga ttcggaaagg ctctacaggg aggcctgcgc ggaaatagta
     3961 ggtcgttacg gcggtgcgat aacgccggag cttttcgaga aacagatggg actgaagatg
     4021 gaggagacgc agagagtggt cgtggaaata gccaatcttc cccttacacc ccaggagttt
     4081 ggaaggatgt acatcgagcg cttcctggag atcgccagag aaaggctcgg tccaaacccc
     4141 ggtcttgtag agcttctgga gtttctcact ccccgcgtaa agctgggtat agcctcttcg
     4201 accgagaagg gcgtgattat ggagatcgtc gagcagatag gtgttggccg ctactttcag
     4261 gcgatagttg ggggagatga agtctccaga tccaagccgg atccggcgat ttaccttcat
     4321 gcggccggtc tgttgggctg cgatccggaa gagtgcatag tgatcgaaga ctccccgaac
     4381 ggtataaaga gcggcacggc ggccggaatg gaagttcttg cagtcctgca cgacgagaac
     4441 agacacctgg atctctcagg cgccggcagg gtattcgaaa acttgatcga agttggagag
     4501 tatctacggc gaagcgacgg actgtcgagt cgcaggcgct aaaccttcac cctgtaccag
     4561 tactgataag cctcttcgaa actgagcgac gagtatagct ttatagctac gtcgttcact
     4621 atatccacct gaagatatgc tacccgggct ccctctctcc cgccgagcgc catcagtttt
     4681 tcggtgagtt ctttgccgta gcccattctc ctgtattcct gaccgatcgt tatgccgaag
     4741 agaccgaagt attcgtcctc cctgacggcc atggcgcaac ctacggcctc ctcgctcttc
     4801 ttcaggatga ggtagtaagc gtcggggact atcgagtcga gtatcctccc cagccagtgt
     4861 tcgttttccg cggctttctt gctcaacctg gtgaaggaac ccagccactc tctggaggga
     4921 gtggtgaaga tgtcgagatc ggatagatcc ctcgtcgctc ctgagagctc ctttgtcagg
     4981 actattgcgc ggtctctttc gacgtaaccc tggctttcca gtgctctgtc cagctcggat
     5041 ggaagagata tggatgtcat cttgaagata gtctggagtc ccctgcttga gcagaaccgt
     5101 tccgcttcgt tgatctttcg catcagatcc tctctcgatt cgtaaagggg gagtatggaa
     5161 ttggccctgt tggtgtaacc cccggcgcac ctcataaccc agccgtcgta gaatcttaca
     5221 tacacacccg gccacgcgtt catggatatc tcttcgagtt tgcgtatgtc cagcatcaaa
     5281 acctccgtgt cgtttgtatg caatattata gcctttctac ggcctctttc tctcgcccct
     5341 cactttctcc gatcgatgat aaaataactg cgtgacaaag ggggtatgaa tggtggaaga
     5401 aaagcgtcta gaccagaacc tgtactcgac atatctctcg agaatcaatt cggtcctcga
     5461 ctacatagag cagcatctgg ccgaaccctt gacactcgaa gaactctccg atgttgccaa
     5521 tttttcgaga ttccactttc acaggatttt ctctgtcttc atgggagaaa cgctttcgca
     5581 tttcatctgg cgtctccgtc tggagaaagc cgccgttctt ctggccaacg actcgaaaaa
     5641 aaccgtctcg gaaatcgccc tggactgtgg attttcaagc ccctcctcat tcagccggag
     5701 cttcaagaag tatttcgaac tcactcctct tgaatggagg aaggaaaaac tcgcacagag
     5761 caatctcgat caagaggaag gcaacttcga tcatacacag agcaacgaga gaaaggcatc
     5821 ttttccggct cctatctaca atcgtagtgt ggaaatcatg atgaggagga cgaacatgga
     5881 aaagatgggc aaaagcgtca ccatcaagca attaccggaa accgacgtgg cctatgtgcg
     5941 ttacatagga ccttacgcgg gcgacgagaa gctcttcgaa actcttttca acaagctttg
     6001 cgcctgggcc ggaccgaggg gacttctcca gagatccgat gcgcagtacc tgattatcta
     6061 tcacgacaac cccgacgtca ccgaagaaga taagttgagg ttgagcgtct gcgtgacggt
     6121 gccgaaaaac acccgggtcg aaggagagat aggaaggatg accatcgcgg cgggagagta
     6181 cgccgcggca cgtttcgaac tggattccag cgagtacgga aaggcctggg actggcttta
     6241 cggccaatgg ctgccccaga gcgggtatct ccccgacgac agactgccct tcgagctgta
     6301 cccgacggac gtaaaaagcg agtgcgaaga caagaaagtg gtggatatct atctgccggt
     6361 caagaagatt tgaagtgtag cggggagtcg atctccccgc ttttattaag gttctccgac
     6421 cacgttgcgt acgaaccagt tcatcgagag gagctcgtcg tccgaaggcc tttctccggc
     6481 cgagcatctc attttaccgc tctgatcgta gagaggtcct tcgaagggat gaagatcgtt
     6541 ggagattatc ttctcccttt cggctctaac attatcggag atagtctgag gtaccagcgg
     6601 gctcatcgat atgtctacga tcgcgtcttt aattccaccc cagtactggt agctcttcca
     6661 gtttttgtcc ataacctccc tcacgacctt ctcgtaaaag acaccccagt tccacaccgg
     6721 agaggccagg aaggttttag gcgaaaacgt gctcatatct gcgttataac ctattgcata
     6781 aactcctcta tctcctgccg cctgaacggc cgccggcgat cagaccggta agataacccg
     6841 gttcgcatat cctgccgaaa taagtcccga ggttctcaga ggttttatag ccggagcaat
     6901 gcatgaaaac cgtttcagga taatgtgccg cgatctccac cacgtcgtcc atgaacccga
     6961 aactcgtact gaagacgata tcgaaaccgt gcccggcaaa acttcgcatg atttcaaggt
     7021 cctgctcacc ttcgaccacg ttttccgcat acccggtctc gatctcacga ccgaaaacct
     7081 tttccagata gagtcgccct tcgtcatgct tttgagacca gccgccgtcg ttggcggacc
     7141 caacatacac aaaaccgact cgaagaggcg ccgcctggat cgatgccgca aacaacagaa
     7201 ttagcaaaga aaccaatctt actttcaagt agacacctcc tttgttctct gcctgccaaa
     7261 acaaacattt ttgcccgcaa cttccgcccc tgtttccgat cgtctggaat tataacagcg
     7321 atcggctaaa gtgggatatc tttcaaacac cctctataac tgaaacgtga gggaagagcg
     7381 gttcatagtt ccgacgctgc gcgccaggtt cttcgttggt taagaagagc gagaagagta
     7441 gagggagact taagagatga gatcccgttt agtaatcctg aataggaaca tttcagggca
     7501 ggctctacgg aatgacagtc ttgctccctt tccgtcatgc cgtacccccc tttcctactc
     7561 gatgccctcg cccacaacgc acaaccaaca acggttcttg agaggattct ctccttctcg
     7621 tgagcgaagc gaactctcga cactctttct catgtttttc acccacaaca gttcatagaa
     7681 agccgactgg cctggtaaaa aaatgacgga ggggttagcc tccgtcgtta tggaattcta
     7741 gatatttttt atttgctaaa gcagcatatc tatgtacgcg gcgtgcgacc agaggagagg
     7801 cgaggcgatc tctccccatt tctcgaccca gggtgcatac attctttctt caagcaggtt
     7861 ttccggaact tgctcgggta gaaaaccttt ggagtcggct tttctctcta tccatgcttt
     7921 gatttcttcg gaaatatctc tttcgttgtt cgatttcaga tgaccggcta gataagcgct
     7981 cagcagtatc cagcttccgc caccgtaata tgtatctttt ttgtaccttt tgaggcctcc
     8041 gtcgaagtag agttcattca tcactgcccg tgc
//
LOCUS       DAOOLY010000100         8068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10477_length_8068_cov_4.469113, whole genome shotgun sequence.
ACCESSION   DAOOLY010000100 DAOOLY010000000
VERSION     DAOOLY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10477_length_8068_cov_4.469113"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..676)
                     /locus_tag="PLD15_06420"
     CDS             complement(167..676)
                     /locus_tag="PLD15_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPB63574.1"
                     /translation="MESNNEKKVLVVYYSYDDSTKTIAEMIADETGADLFELVPLEES
                     GGKRVKYMWEGESVIMNPTPRLKATPDNLGDYDLIFFGTPVWAMSYAPPFESLFNSTR
                     LHGKNVALFCTHEGLMGIVFQEMINRLKDNKIVGTVDFYDPVGSGIEYAANAGRNWAR
                     HVLKSILES"
     gene            complement(917..1663)
                     /locus_tag="PLD15_06425"
     CDS             complement(917..1663)
                     /locus_tag="PLD15_06425"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HPB63575.1"
                     /translation="MESAELFWKASIEDLKRGYSLDERNYVCHYCGMSFEEGVVYTVN
                     GVFFDAKRTVEEHIKSVHGSVFESLMAIERKYTGLTELQCELMGGFFSGLDDGEIARR
                     MNINRSTVRNHRFRLKEKAKQAKIFLVLFELMEEKIKKEDRIIDVHRGATMIDDRYVI
                     TEAEREEKIKKFFGEDGSLIRFPKKEKDKLIVLMKIAGEFTSNRIYSEKEVNEILKGY
                     YDDYVLLRRYLIEYGFITRKPDGSSYWSIF"
     gene            complement(1886..2806)
                     /locus_tag="PLD15_06430"
     CDS             complement(1886..2806)
                     /locus_tag="PLD15_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63576.1"
                     /translation="MEILPYSKDRQDEIMSVEKLYEEKYPLAESFDVSDLKNPLLNGR
                     RNILLALDDGKVVGFSKFLSRQNFSNKYYHHVWLDIKTILFDRALEVMGELFQSSIRP
                     ISKSTESYFRAAGTRICVRLHESEIERYRFFEGLGFLPWVDFYYMERDLELPVIPVKF
                     PEGLLVKVWKPKKEPEILKYLSAEQSCFPDSPLEYRYLNYFFQRPDWKSHGAVIAVFD
                     DRKNLVASLMVYPSNDGEYYTEEVFVVNKWRGKGVARALMSEALIYLQNRQARRALLS
                     VTSDRTTAMKIYRECGYDHTFTRKVLVLTV"
     gene            2912..3466
                     /locus_tag="PLD15_06435"
     CDS             2912..3466
                     /locus_tag="PLD15_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPB63577.1"
                     /translation="MESILIAFGLAMDAFAVSICVGVSLEKITPGQIFRVSFHFGFFQ
                     FMMPLLGWLLSSRFEKYIESFDHWIAFALLSFIGGKMFLEVFKKEEESLCIDRTRGVH
                     LIMLSIATSIDALAVGIAFAFLKKPILEPAIVIGVVTASLSLLGIKGGHRLGEKFGKS
                     MQAIGGLVLVSIGAKILIEHVFSH"
     gene            complement(3516..4715)
                     /locus_tag="PLD15_06440"
     CDS             complement(3516..4715)
                     /locus_tag="PLD15_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63578.1"
                     /translation="MRKNIFLLLIYTFISTITISMYRVVYNLYLRELGFCNNTIGNIT
                     SAQLWGSAIIGLIAAVIADRIGKRKILLFSAVVVPVVGIALAYVVDPGIVVLLSFIKG
                     GFTVTAFTVVMAAMTGVTKTENRAKIFGLNFGINMASGVVGNFVGGFFGDLFGLQSTL
                     LISMLGHFLAVIPIIQLRVIDTKSRFKELFDFSGLERDEKKVLTYYFVSTATVGFGAG
                     LFIHFGNLIFKDLFNMSATGIGIALSIAQFGTAAGSILSHRLGRRFGPLRFNLAMQLL
                     VVPLMLSLVVARQPVLFTVLYAFRFVFMNITNPIMTSIIYSYVPNSKLSTVSGLNGFL
                     NNTVRAIAAMVFGYIVGTYVSGYNQLFLLSTVFYGANAFVIFLFYREFGTKSKVMELY
                     KERNSRS"
     gene            4854..7181
                     /locus_tag="PLD15_06445"
     CDS             4854..7181
                     /locus_tag="PLD15_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB63579.1"
                     /translation="MTQSKLRRIGSEVLKPLKKVVEKAVAFSVALPWMILILFAVLTV
                     FFGMKTTSLKFETNIDNLATKSASEIKEIELAARDFSIWEPLYLVLVGDTRKLETWQK
                     IAQTTANLRKVENVSRALSPLDSTYITLQGFSIGNIPVASAVPSSEGELKIFFDNLSR
                     SFDSRFSVSSSGDAVVVRLYIRGGLGSRGKKAIDGIQKILQDEWGDGGYYLTGELFLG
                     YSVDKTVVADVMILFPLAIAVVFMVTILSFRSVSGVLAPTLTVLSTVVVTLGFMAMLG
                     KPLTIISAILPVLLIAMSSADSIHIFSRYREEISRGSQKRQAITQSMSSMVLPVVMTS
                     LTTAAGFGSLIFSSIIPMKELGYFSVFGIIYAMLFSLLAVPAVLSSLPAPRVDIAASR
                     NVEEPFMDKLLYRFSVAVAKKRRIVLLISIAVALVCIVGITNVQFESNLSRYFRKGSA
                     VSRGVTTFEDRFGGSSLLIIAVDSGREGGVMTVDFIRALGEMEKVLDGFDILSRTSSF
                     HSLVGSIYPGELNQGQITLIKSGVGSSGVQGYVSKDSSKAAIHTYIRNTSTREISRTL
                     VRVERELLKVLPAGATLTLTGTPRVIQLHMESFAQSQAVSLLFSAIIVWFIVSLMFLS
                     IFIGSLAMLPLLFTIVFSLGIMGLTGTPLDAATVLVASISVGVGIDYSIHFIERVKYE
                     LKSGNSLEKASNKASRTAGRAILINAITLISGFSILAFSNFLTVSAFGILMTFTMAAS
                     SVAALVIIPAILNSRRLGDRILKVFLRADVKESRQ"
     gene            complement(7202..>8068)
                     /locus_tag="PLD15_06450"
     CDS             complement(7202..>8068)
                     /locus_tag="PLD15_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63580.1"
                     /translation="IASYPGWYTYFSDSPDPSYNQWKNDYLNSLPAGGTDYLLSSNHT
                     LSSVNESELIIISPPTGAVSPLFRAFFDDSGLKIYYGDSGNFLHIPVSLVRSTTANVK
                     IMGDTVFGNDPHGISKVCGHYNISVYGDITINTNIVYDYLYDFVNNGDGNSPVGNKTK
                     EVSQDIISNMLTKLRTSSLTLASVGGDMIMQYIHGNSTHGNKSLTGTFFVWKDSGSGG
                     DVQFPDIQNLITGNGHTPQLFILGSITAYTFDEQGQATGLDSFVAVADAYVEDLVSSG
                     LGSLGLVGIQTW"
BASE COUNT         2028 a         1984 c         1930 g         2126 t
ORIGIN      
        1 tgaaaggagg gaagcgtcat tcctcaccgc gctgaagcac ggtgtatcct gacgctgttt
       61 agatgaaaat tgaccggaaa tacaccaata ccgcattccc ggccttttat catccatacg
      121 cttcaactgt acatatcaca tcgaatacgg ataacttctt tggccgtcac gattccagga
      181 tcgatttaag gacgtgtctg gcccaatttc taccggcgtt tgccgcgtac tcgattcccg
      241 aaccgaccgg atcgtagaaa tcaaccgtcc ccactatctt gttatctttg agcctgttga
      301 tcatctcctg aaagactatt cccatgagcc cttcgtgtgt gcagaaaagc gcgacgttct
      361 ttccatgcaa ccgggtggaa ttgaacagac tctcgaacgg cggagcgtag ctcatcgccc
      421 acaccggagt tccgaaaaat atgaggtcgt aatctcccaa attatccggt gtcgctttca
      481 aacggggagt gggattcatt atgacacttt cgccctccca catatacttc actctctttc
      541 caccgctctc ctccagcgga accagctcga agaggtcggc gccggtttcg tcggcgatca
      601 tctcggctat ggtttttgtg ctgtcatcat aggaataata aacgacgagt accttctttt
      661 cattgttcga ttccatttat aagacctccc aatggcgata aatattatcc atatttattt
      721 tcgcacatcc cgtccgcttt ttcaaacaga gttccggagt ctcaggattg atcagcccgt
      781 ttcatcgtgg ttcacaccac cagtgcgaga attaagagcg gttcgccgtt gcttgcgcgg
      841 aacgctgctc catattaatt aacacgcctt tcgccgctcg aaagagcgag atgaaaggcg
      901 ggagatcgta aaaaaatcag aaaatcgacc agtagctgct gccatcgggc ttgcgggtta
      961 tgaagccgta ttcaatcagg tatcttctca acagtacata atcgtcgtag tagcctttca
     1021 agatttcgtt aacctctttt tccgaataga tgcggttgct ggtgaactcc ccggcgatct
     1081 tcatgagaac tatcagcttg tccttttcct tcttgggaaa cctgatcaag ctaccgtctt
     1141 ccccgaagaa cttttttatt ttttcttccc tttccgcttc ggttatcacg tatctgtcgt
     1201 cgatcatggt agcacctctg tgaacatcga ttatcctgtc ttccttcttg attttctctt
     1261 ccatcaactc gaaaagaacc aggaagattt tcgcctgttt ggccttctct ttgaggcgaa
     1321 accgatgatt tctgactgtt gaacggttga tgttcattct cctggctatc tctccgtcgt
     1381 ccagaccgct gaaaaatcct cccattagct cacattggag ttcggtcaga ccggtgtact
     1441 tcctctcgat cgccatcaag gattcgaaaa ccgaaccgtg cacggacttt atgtgctctt
     1501 caacggtcct tttcgcatcg aagaaaaccc cgttgaccgt gtaaacgact ccttcttcga
     1561 aactcattcc acagtaatga cagacgtaat tcctctcgtc aagggaataa ccgcgtttaa
     1621 gatcttctat actcgccttc cagaaaagtt ccgcagattc catatctaca cctccatgga
     1681 acaatatacc acaccacgaa taaataatca aacaagaatc tattttgttt gattatattc
     1741 aactaattag ctacattgtt tgttataaag tggcatctct gtacacagac gggccgttgt
     1801 tgcgttcggc gttgctgagg tcccggttga aaagataatc gatcgcacgt tgttgtttgt
     1861 ggcattaaaa aaagaacatc tgtcttcaga ccgttaagac aagcactttt ctggtgaaag
     1921 tatggtcgta cccgcattcg cggtagatct tcatcgccgt ggtcctgtcg gaggttacgg
     1981 aaaggagagc cctcctggcc tgtctattct gcaaatatat gagagcttcc gacatcagcg
     2041 cccttgccac tccctttccc ctccatttgt tcacgacaaa aacctcttcg gtgtagtatt
     2101 cgccgtcatt cgatggatac accatcaaac tcgcgacaag gtttttcctg tcatcgaaga
     2161 ccgctataac ggccccgtgg gatttccagt ccggtctctg gaagaagtaa ttcaaatacc
     2221 tgtattccag tggagaatct ggaaagcagg actgttcggc gctcagatat ttgaggatct
     2281 ccggttcttt ctttggcttc cagactttca ccaacaggcc ctcggggaat ttcacaggta
     2341 tcaccggaag ttcgagatct ctttccatgt aatagaaatc gacccatgga aggaatccca
     2401 gtccttcgaa gaacctgtat ctttcaatct cgctttcgtg aagccttaca cagattctcg
     2461 ttccggcagc cctgaagtaa gactccgtag atttacttat cggacggata gaactttgaa
     2521 aaagctctcc cataacctcg agagctctgt cgaagaggat ggtttttata tctagccaca
     2581 cgtgatgata gtatttattc gagaagttct gtcgcgaaag gaatttcgaa aaaccgacga
     2641 cttttccatc atcaagtgcg agaagaatgt ttcttctccc attgaggagg gggtttttga
     2701 ggtctgaaac atcgaagctc tcggcgagcg gatacttctc ctcgtagagc ttttccaccg
     2761 acattatctc gtcctgtctg tctttcgaat agggcagtat ctccatcgct ccacctcccc
     2821 caagagtata tcaaaacttc tttatcgatt caggaacacc ccgcgtattt atgctagcat
     2881 tgtgaaaaga cttacagcgt cggggtttgt gatggaaagc attctcatag cttttggtct
     2941 ggcaatggac gcgttcgcgg tatcgatatg tgtcggtgtt tctctggaga aaatcacacc
     3001 cggacagatc ttcagggtgt ccttccattt cggattcttc cagttcatga tgcctttact
     3061 cggatggctt ctgagctcga ggttcgaaaa gtacatcgag tctttcgatc actggatagc
     3121 cttcgcactc ctctctttca tcggaggcaa gatgtttctc gaagtcttca agaaagaaga
     3181 ggaaagcctg tgcatcgata gaaccagagg agtgcatctt ataatgctat cgatagccac
     3241 ttcgatagat gcgctcgcgg tggggatagc cttcgctttt ttgaagaaac ccattctgga
     3301 accggccatt gttataggcg tggtaacggc ctctctctcc ttacttggca taaagggagg
     3361 tcacaggctt ggcgaaaaat tcgggaagtc gatgcaggcg ataggcggtc tggtgctggt
     3421 atcgatcgga gcgaagatcc ttatagagca cgttttcagc cactgagggg aacagagaga
     3481 ttaaaaagtc aagatctcat cgctcttcac acctttcagg atcgggagtt ccgctctttg
     3541 tacagttcca tgaccttgct cttggtcccg aattcgcggt agaagaggaa gattacgaac
     3601 gcgtttgccc cgtagaatac ggtgctgaga aggaagagct ggttgtatcc gctcacgtat
     3661 gtaccgacaa tgtacccgaa gaccattgcg gcgatcgctc tgaccgtgtt gtttaaaaaa
     3721 ccgttcagac cggacactgt ggacagtttc gaatttggaa cgtaggaata gattatggaa
     3781 gtcattatag ggttggttat gttcatgaac acgaacctga aggcgtacag cacggtgaag
     3841 aggaccggct ggcgcgccac cacaagagag agcataagcg gaactaccag cagctgcatc
     3901 gcaagattga acctcaaagg tccaaaacgc ctgcccagac ggtgagacag gatggaaccg
     3961 gcggccgttc caaattgcgc gatcgagagg gctattccaa tacctgtagc cgacatgttg
     4021 aagagatctt tgaagatcag attcccgaaa tgtatgaaaa gcccggcacc gaagcccacc
     4081 gttgccgtag atacgaagta gtaagtcaga accttttttt cgtcacgctc aaggcccgag
     4141 aagtcgaaga gctctttgaa tctcgacttt gtatcaataa ctcgcaactg aattatggga
     4201 attacggcca gaaagtgccc gagcatcgaa atcaaaagag tcgactgcaa tccgaagaga
     4261 tctccaaaaa agccccccac aaaattacca acaacaccgc tcgccatatt tatgccgaag
     4321 ttcaacccga agattttcgc cctgttttcc gtcttcgtga ccccggtcat agcggccatc
     4381 accactgtga aggccgtcac cgtgaagcca ccttttatga atgaaagcaa caccacaata
     4441 cccgggtcaa caacgtaagc cagggcgatt ccaacaaccg gcacgacgac agccgaaaag
     4501 agcagtatct ttctctttcc gatcctgtcg gcaattacgg ccgctataag acctattatg
     4561 gccgaacccc acagctgggc cgaagttatg ttgccgatgg tgttgttgca aaaacccagt
     4621 tctctgagat aaaggttata aactacccgg tacatggaga tggttatcgt tgagatgaac
     4681 gtgtaaatca ggagcagaaa gatgtttttc ctcaaaccgc aaccttcttt tgtttgatat
     4741 gctgacaata tactaccact tcgagcagta actttgaagt accgactgag ccttagagcg
     4801 ttcaacatga tatgatactt ttcaaagaac gtctattccg ggagggttat agattgacgc
     4861 agtcgaagct acggagaatc gggtcggagg ttttgaaacc cttaaagaaa gttgtcgaga
     4921 aggccgttgc tttcagcgtg gcgctaccct ggatgatact gattctcttc gcggtgctca
     4981 cggtcttctt tggaatgaag acaacttctc tgaagtttga aacgaacata gacaatctag
     5041 ccacgaaatc ggctagcgaa ataaaagaga tagagctggc ggcgagagac ttctcgattt
     5101 gggaaccgct ctatctggtt ctcgttggtg atacgagaaa actggaaacc tggcagaaga
     5161 tagcgcaaac cacggcgaac ctgcggaagg tagaaaatgt atcgcgcgct ttgagtcctc
     5221 tagattcgac atacataaca cttcaaggtt tcagcatcgg gaacatacca gtagcctccg
     5281 cagttccttc aagcgaagga gaactcaaaa tattttttga taatctctcc aggagttttg
     5341 acagtcgctt ctccgtcagc tcttctgggg atgcggtcgt tgtgaggctg tacattaggg
     5401 gcggtctggg aagccgtggg aagaaagcca ttgacggaat acagaagata ctgcaagatg
     5461 aatggggcga cggaggttac tatctcacag gagaactctt tttgggttat tcggtcgata
     5521 aaacggttgt tgcagatgtg atgattctct tcccgctggc catagccgtt gtctttatgg
     5581 tcactatact gagcttcaga agtgtctcag gagtgctggc tccaaccctc actgtgcttt
     5641 ccactgtcgt ggtgactctg ggcttcatgg ctatgctcgg taaacccctc acgataatca
     5701 gcgccatcct gcccgttctg ctgatagcaa tgagcagcgc cgacagtata catattttca
     5761 gtagataccg cgaggaaatc tcgcgtggta gtcagaaaag acaggcgatc acacaatcga
     5821 tgagctccat ggtgctgccg gtagttatga cctcgctgac gaccgctgcc ggttttggct
     5881 cactcatatt ctcttccatc attccgatga aagaacttgg ctacttcagc gttttcggaa
     5941 ttatctacgc tatgttgttt tcattactgg ccgtccccgc cgtactttct tcgcttcccg
     6001 caccgagggt tgatatagcc gcgtctagaa acgtagaaga gcctttcatg gataagttgc
     6061 tatacagatt ttccgtggcg gtagctaaga agaggcggat cgtgttgctg atttcgatag
     6121 ccgttgctct ggtctgtata gtcggtatca caaacgtaca gttcgaatcg aatttgtcaa
     6181 gatacttcag aaaggggagc gccgtatcca ggggcgtaac cacctttgaa gatagattcg
     6241 gcggatcctc ccttctcata atcgccgtcg attctgggag agaaggaggg gtaatgactg
     6301 tggatttcat aagggcgctc ggcgagatgg aaaaggtgct ggacggtttt gatatactgt
     6361 ctagaacgtc ttctttccac agcctggtgg gctcgatcta cccgggagaa ctgaaccagg
     6421 ggcagataac gcttataaaa tcgggcgtcg gaagctcggg ggtgcagggg tacgtcagca
     6481 aggattcctc gaaagccgcc atacacacgt atatcagaaa tacaagtacg cgggagatat
     6541 ccagaacact tgtccgggtc gaaagagaac tgctaaaggt tttaccagct ggcgccacgc
     6601 tcacactcac gggtacacca cgcgtcatcc agctacacat ggagagcttc gcccagagcc
     6661 aggcggtcag ccttctgttc tcggcgatta tagtctggtt catcgtatct cttatgttcc
     6721 tgtcgatttt cataggttct ctcgccatgc taccactact tttcacgata gttttcagtc
     6781 tgggaattat gggattgacg ggaacacctc tcgatgcggc aaccgttctg gtggcaagca
     6841 tatccgtcgg agtcgggata gattactcga tacatttcat agaaagggta aagtacgaac
     6901 tcaagtctgg caactcactg gaaaaggcca gcaacaaagc ttcccgcacg gctggacgcg
     6961 caatactcat aaatgccata actctcattt cgggcttttc gatactcgcc ttctccaact
     7021 ttctcacagt atcggcattt ggaatactga tgaccttcac gatggccgcc agttccgtgg
     7081 cggctctggt gatcatacct gccatactga actcgcgaag gctcggcgat aggatattga
     7141 aagtcttttt aagggcagac gttaaagagt cgagacagta gaaatgacgt tcccaaagca
     7201 cctaccaggt ttgaattccg accagtccca ggctaccgag tccgctggaa acgagatcct
     7261 cgacgtacgc gtcggccacc gccacgaaag aatccaggcc ggtcgcctgt ccctgctcat
     7321 cgaaagtata ggccgttatt gaacctagta tgaacagctg gggagtatga ccgtttccag
     7381 taatgagatt ttgaatatcc ggaaattgta catctcctcc gctcccggaa tccttccaga
     7441 cgaaaaaggt tcccgtgagt gatttgttgc cgtgagttga gttcccatga atgtactgca
     7501 tgatcatatc tccgccgacc gaggcaaggg tcagagagga tgtccgtaat ttggtgagca
     7561 tattgcttat tatgtcttgc gatacttctt tggtcttgtt gcccacagga gagttgccgt
     7621 cgccgttgtt cacaaaatcg tacagataat cgtacacgat gttggtgttt atagttatat
     7681 ccccgtaaac cgaaatgttg taatgaccgc agaccttgct gataccgtgt ggatcgttcc
     7741 cgaagacagt atcgcccatt atcttgacgt tcgccgtggt gcttctcaca agcgacacag
     7801 gtatgtggag gaagttcccg gaatcgccgt agtatatctt cagaccgctg tcatcgaaga
     7861 aagctctgaa gaggggagag acggcccctg tggggggcga tattattatc agctcgcttt
     7921 cattcaccga cgagagcgta tgatttgaac tgagaaggta atcggtgccc ccagcgggca
     7981 gagagttgag gtaatcgttc ttccactgat tgtaggaggg atctggtgaa tcggaaaagt
     8041 acgtgtacca gcccggatag cttgcgat
//
LOCUS       DAOOLY010000101         8065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10480_length_8065_cov_5.334956, whole genome shotgun sequence.
ACCESSION   DAOOLY010000101 DAOOLY010000000
VERSION     DAOOLY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10480_length_8065_cov_5.334956"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLD15_06455"
     CDS             complement(<1..562)
                     /locus_tag="PLD15_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63581.1"
                     /translation="MKVSTDKRIRLVSTAMMLTELPELELRKPHAHHFLYEDTMRHLA
                     GRKQPELLNSLLKDGTAYYFLFTYAVNLSWPDLEPLSFPDYLYRYEPALCASKLHLEL
                     RELLASADLESLWEGQKTGWEKLERDASTALEDNRIEELLRDFFGDHGRNLFFFPNPL
                     FPELASLGAASDNCLYCIARYPGRSSQ"
     gene            complement(565..1668)
                     /locus_tag="PLD15_06460"
     CDS             complement(565..1668)
                     /locus_tag="PLD15_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPB63582.1"
                     /translation="MIFDWTVQESFLKAFPQKVGTMLDTLKGHIDKGRDSLDEDAVHD
                     TRVWSRRLSGALSIGAILIDRKPSRSLEMLSALRTSLGRLRDNHVQRSLAIEYGIEGL
                     PEYLEAKGERLAAQASKSLADFDMERLERDFLHFHRELIVVCNDGNLLKQNTERISNR
                     IEKRYSSVGKSFTGSDPADFATLHSSRIAIKKLRYTLETLDGYTGLDGDTLALLKKIQ
                     DRLGKVQDLTVLLKTIGKLVNKRRLSVPGETVQRISIDRHMLVSEFLDGWRDELAAIG
                     RFIEEPATKQCEREDKDRRNLRSGAVEKFSDGIIEKILSLADEKGLSYRGVVAYLREN
                     PAVRDKLDIESLPSYQTLARRAGKLGKRKKPGG"
     gene            complement(1665..2159)
                     /locus_tag="PLD15_06465"
     CDS             complement(1665..2159)
                     /locus_tag="PLD15_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB63583.1"
                     /translation="MRRIFVVRHAEAEKGKKGDMQDIDRPLTDSGKTDSLSMAEIVKP
                     FIDGPAIIISSGAKRAMQTARKFRKAIGGIKLIENGELYTGTGETIAATITATDDKYN
                     CVIIVGHNPAIEEFMAAARCVARMATSSVAFFKVDCDSWKELDLNKFKLEFLLKPSLL
                     RKTK"
     gene            complement(2261..3094)
                     /locus_tag="PLD15_06470"
     CDS             complement(2261..3094)
                     /locus_tag="PLD15_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63584.1"
                     /translation="MIRELELKDRKSVLEIVKDIWEGDDYIPAAFDSWVSDGDCRFMG
                     MWKDEALVGVANLRRLSPTVGWLEGMRIKPEFQGKGYGRELAVEMMKLARNDRLEELY
                     FSTYFDNLASIRINESLGFSRVAVFTNLELELKPSADLEGELKYAGLQIGREIAEIET
                     YMSNDWFFIPPEIGRKSNWLKEPETLSREGNMAVVSRNPKVPECMEINYIEIGKSEDA
                     ENFLSLLAGYAREKGYSRVHTMVPENVELDAFLKNRFAPFERFHDVFIYRARTDELRI
                     D"
     gene            complement(3091..4161)
                     /locus_tag="PLD15_06475"
     CDS             complement(3091..4161)
                     /locus_tag="PLD15_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HPB63585.1"
                     /translation="MAYVEINKSNISKNARHLLNLTGRAGVEMASVTKVVCGDPEIVS
                     KIIEAGVREIGESRLENIDRIRKFGLSPAFLLLRLPSPQLYRKVVESVDSVLLSELKS
                     LKALKELSEEMGRRLKFIYMVDTGDLREGVMYDRAFEELGEAAKIAGRSLDGIGTNLG
                     CFGGVIATPEKFEILLALGDRLRREDGIEIERYSAGNTASLPLIENGTLPRGINHYRL
                     GESIMCGTDVTNSRAVPGTTRETFTLYGTVLELKRKPSVPIGEIGSDAFGRKPHFEDM
                     GERLKAILDIGEQDVVPSGLTPMLEGCRVLHASSDHLIVDVTDCPEKLSVDDRIPFRM
                     SYGALLRVMTSRYIAKEYRDLS"
     gene            4257..4679
                     /locus_tag="PLD15_06480"
     CDS             4257..4679
                     /locus_tag="PLD15_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HPB63586.1"
                     /translation="MDANRGVYLVLLELEHRTLISTGAREWELEPGMYIYVGSAMNSL
                     SERIGRHLRLEKRKHWHIDYLREKSRVLAALLLPSDRRMEEELSRFVSKYGESVKGFG
                     ASDCNVESNLYRIELEAVSEVFYAIVKNWRERDDSFFD"
     gene            4660..5445
                     /locus_tag="PLD15_06485"
     CDS             4660..5445
                     /locus_tag="PLD15_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPB63587.1"
                     /translation="MIAFLTDWGYDSYYVGIAKAVVREINRDVEILDICHNIKPFSVR
                     HAAHVIERAIKDLPADTVFLAVVDPGVGTSRKPVAMVLKNGMCMVGPDNGIFTLAAES
                     FGVHEVRELENRQYHRGVSRSFHGRDIFAPVAAHIAKGLAVEKLGSRLMNFEYLKYRK
                     ATRSGDALTGEVAFYDGFGNLETNIPLSLGTGLEEGDILELSRGRKQFTITFGNTYYD
                     VNFGQLMAHFDSSGYMEITVNKGSARELLGMDEGEQITVVKAR"
     gene            5429..6739
                     /locus_tag="PLD15_06490"
     CDS             5429..6739
                     /locus_tag="PLD15_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB63588.1"
                     /translation="MSKPDKVLFGSGYHYITGIVVAGAGLLASLLIGFNVESLLLVIF
                     SGLSATAFVYLVIVRQLNRLVKLIRSGERKPAAELEGQFHELYGFYRNLSLQVEQERN
                     YYEKLYRDYSELLNTLNISVVSLDGSGNIDLANEAFVKSFSYGAQNMRYLKLDHFIRR
                     TGLLFPLQEGQYDVYSKKLGRRYLVTVTKKKRSGFLVTLDDKTSQWKTRQLLEKTRRY
                     AVDAQTVADLAHGLKQPLANMRLALDLYIRSRKDSYLQTLSNELSGFQVKVGGILQIF
                     RYGEEFERLDLSESVRKVLSFMEPILAARSISLDTISLDDGKVLVQKGRLENVVKNLL
                     MNAVEASEDGGEIALKVRRSSEYLTLIVSDRGKGIDESIQAEVFKPFYTTKSEGSGLG
                     LYLIKNFCDENDVRLKMKSVPGKGTVFVLTFRRNDDESQDSSGR"
     gene            6711..8057
                     /locus_tag="PLD15_06495"
     CDS             6711..8057
                     /locus_tag="PLD15_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB63589.1"
                     /translation="MNRRILLVDDDLAFNGLLAAALREEGYDVTQVYNLKQAEKSLLD
                     EFFDCVILDVRLPDGSGLDLLPDAAQTAPVIVVSAHGDINTAIDAVRKGAFNFLEKPF
                     DLTHALLEVKRSIEYSELSRERDDLAGMVGGDTLSEFVGKSPSVIRLKETISMIAPKK
                     VTVLLEGESGTGKEIVARAIHQLSGRKRFVAINCGAIPENLFESELFGYEKGAFTGAL
                     HSRPGLIEESSRGTLFLDEVSEMPLALQVKLLRVLETSTNQRLGGNISRRLDLRVIAA
                     TNRDLESYVTAGQFRSDLYYRLNVVKLKIAPLREKREDIPVLVEYFLPRLCQELGLRK
                     NPMIPAGLLDEMMGYDWPGNIRELRNKLLSMLAVNGDREILSSIGLENPENEKCVEKG
                     FEEVSLDEMERRYISWLLKRHSGNKSETARVLKISKSTLYEKMKRWESFEGDNDPS"
BASE COUNT         1837 a         2074 c         2226 g         1928 t
ORIGIN      
        1 tctggctgga cctaccgggg tatctggcta tacagtatag acaattatcg ctggccgcac
       61 ctagagaggc cagttcggga aagaggggat tggggaagaa aaagagattt cttccgtgat
      121 cgccgaagaa atctctcagg agctcttcta tgcggttgtc ttcgagcgcc gtcgaggcat
      181 cccgctccag tttctcccag ccggttttct ggccttccca cagactttcg agatcggcgg
      241 aagccagtaa ctctctcaac tcgaggtgaa gtttgctggc gcaaagcgcc ggttcgtatc
      301 ggtaaaggta atctggaaag ctcaggggtt cgaggtcagg ccacgatagg tttacggcgt
      361 aagtgaagag aaaataatag gccgtcccat ctttgagaag cgagttgagg agttcaggtt
      421 gctttctgcc cgccaggtgg cgcatcgtgt cctcgtatag gaagtgatgg gcgtggggct
      481 tgcgcagttc cagctccggc aattcggtca gcatcatcgc ggttgataca agcctgatcc
      541 ttttgtccgt agatactttc aagatcaacc tcccggtttc tttctcttcc ccagcttgcc
      601 ggccctcctg gccagcgtct ggtatgaagg aaggctttcg atgtccagtt tgtcgcgcac
      661 ggccgggttt tccctgaggt aggccacaac gcctctgtaa ctcagtccct tctcatcggc
      721 cagagagagg atcttctcga taatcccgtc gctgaacttt tcgaccgcgc cggaacggag
      781 gtttctcctg tctttatcct ctctctcgca ctgctttgta gccggctcct cgatgaaacg
      841 gccgatggcg gccagctcat ctctccagcc gtcgagaaat tcactcacca gcatatgtct
      901 gtcgatagag atcctctgaa ctgtttcgcc cggaacggag agccttcttt tattaaccag
      961 tttacctata gtcttcagca gtacggtgag gtcctgaacc tttccgagtc tgtcctggat
     1021 ctttttgagg agcgcgagcg tatcgccgtc aagccctgtg tatccgtcaa gtgtctcgag
     1081 tgtgtaacgg agcttcttta tggcgatcct ggagctgtgg agcgtggcaa aatcggcagg
     1141 gtccgacccg gtgaagctct tcccgactga cgaataacgt ttttctatcc ggttagatat
     1201 tcgttctgtg ttctgtttca ggagattccc gtcgttgcag accactatga gctctctgtg
     1261 aaagtggagg aagtctctct cgagacgttc catatcgaaa tcggccagcg atttggaggc
     1321 ctgcgccgcg agtctttcac ctttggcctc caggtattcg ggaaggcctt cgataccgta
     1381 ttcgatggcc agagatctct ggacgtggtt gtccctgaga cgaccgagcg aggtcctgag
     1441 agccgaaagc atttcaagag atctggaggg ctttctgtcg atgagaatgg cgccgatcga
     1501 aagcgccccg gaaagtctcc ggctccacac cctcgtatcg tggacagcat cttcgtccag
     1561 cgagtctctt cccttatcga tatggccctt gagagtgtcc agcatcgttc cgaccttctg
     1621 cgggaaggcc ttgagaaaag actcctgaac ggtccagtcg aagatcattt cgtctttctt
     1681 aatagcgacg gcttgaggag aaattcgagc ttgaacttgt ttaggtccag ctctttccag
     1741 ctgtcgcaat cgaccttgaa gaaggccacc gatgaggtcg ccattctggc gacgcaccgg
     1801 gcggctgcca tgaactcttc gattgccggg ttgtggccga ctataatcac gcagttgtac
     1861 ttgtcgtccg ttgcggtaat cgtcgcagct atggtctccc ccgttccggt gtagagctca
     1921 ccgttctcga tcagcttgat ccctcctatg gctttccgga actttctggc cgtctgcatg
     1981 gcgcgcttcg ctccggagga gattattatc gccggtccgt ctataaacgg ttttacgatt
     2041 tcggccatcg acagtgaatc ggtctttccg ctatcggtga gcggcctgtc tatatcctgc
     2101 atatcgcctt ttttcccttt ttcggcctct gcgtgacgta ctacgaaaat tcttctcaat
     2161 cattccacct ctttagtgaa ccaaagtcgt tatccgttgg aacgtacgag aacaatgata
     2221 ctatctctcc ttccagaaaa ccaagagagc tagactctct tcagtctatt ctcaattcgt
     2281 cggttctggc ccggtatatg aaaacatcgt gaaacctctc gaagggtgcg aaccggtttt
     2341 tcaaaaaggc gtctaactca acgttttcgg gcaccatcgt atggacgcgg gagtaacctt
     2401 tttcgcgggc gtacccggca agcaacgaaa gaaagttttc ggcgtcttcg ctcttgccta
     2461 tctcgatgta gttgatctcc atgcattcgg ggactttcgg atttctggag actacggcca
     2521 tattgccctc ccggctcaag gtttccggtt ctttcaacca gttgcttttg cggccgattt
     2581 ccggggggat gaagaaccag tcgttgctca tgtaagtctc tatctccgcg atctcccggc
     2641 caatctgcaa tcctgcgtat ttcaattcgc cctcaagatc cgcggagggc ttgagttcca
     2701 gctccaggtt cgtaaagacg gcgaccctgg agaatcccag cgattcgttg atcctgatcg
     2761 aggccagatt gtcgaagtaa gtcgaaaaat aaagttcttc cagcctgtca ttccgggcca
     2821 gtttcatcat ttctacggcc agttctctgc cgtatccctt cccctgaaac tctggcttga
     2881 tcctcattcc ttcgagccag cccaccgtcg gcgagagacg cctgaggttg gccacaccaa
     2941 ctagcgcttc gtccttccac attcccatga aacggcagtc gccgtcgcta acccacgagt
     3001 cgaaggcggc cgggatataa tcgtcgccct cccatatgtc tttgacaatc tcgagaacac
     3061 ttttcctgtc tttaagctcg agttctctaa tcatgataaa tccctgtact ctttggcgat
     3121 atagcgggag gtcataaccc ttagaagggc tccgtaagac attctgaacg gtatcctatc
     3181 atccaccgaa agtttttccg gacaatcggt aacgtcaact atcagatgat cgctggatgc
     3241 gtggaggacc cggcaacctt caagcatcgg tgtgaggcct gatggaacga catcttgctc
     3301 accgatatcc agaatggctt tcaacctctc gcccatatcc tcgaaatggg gctttcttcc
     3361 gaaggcatcc gatcctatct ctccaatcgg gacggacggt tttctcttga gctccagaac
     3421 agttccgtag agtgtgaagg tctccctggt agttccgggt acggcgcggc tattggtcac
     3481 gtcggtgccg cacatgatgg attcgccgag tctgtaatgg ttgatccctc gcgggagtgt
     3541 tccattctct atcagcggta gcgatgcggt gtttccagcc gaatacctct cgatctcgat
     3601 accgtcctcc ctgcgcagcc tatcgcccag agcgagaagt atctcgaatt tttccggcgt
     3661 tgctatcaca ccgccgaagc agccgaggtt cgtgccgata ccgtctaaac tccgccccgc
     3721 tatcttcgcg gcctctccaa gctcttcgaa ggcccggtcg tacatcacgc cctctctaag
     3781 gtcaccggta tccaccatgt agatgaactt caagcggcgg cccatctctt ccgagagctc
     3841 cttgagagcc ttcagggatt tcagttcact aagaaggacg gaatcgaccg attcgacgac
     3901 ttttctataa agttgcggag atgggagacg caacaggaga aaggccggcg agaggccgaa
     3961 cttcctgatt cgatctatgt tctccagtct cgattcgccg atctctcgca caccggcctc
     4021 gattatcttc gaaactattt cagggtcgcc acagacgacc ttcgtgacgg aggccatctc
     4081 cacaccggct cttcccgtaa gattcagcag atggcgggcg tttttcgata tgttcgattt
     4141 gttgatttcc acgtaagcca tgcgatcacc tctcaaggaa ttatacgcaa cccgacggac
     4201 aactttcaag gagtctcttc tatcatattc gatcgctatc gtataatagc tacgtcatgg
     4261 atgctaacag gggagtttat ctcgttttgc tcgagcttga gcatagaacg ttgatttcga
     4321 ccggtgcaag ggaatgggag ctcgagccgg ggatgtatat atacgtggga tcggcgatga
     4381 actcgctctc tgagagaatc gggcgccatt tgaggcttga aaagaggaag cactggcaca
     4441 tagactatct cagggagaag tcgagggtcc ttgccgctct tctccttccc tccgacagga
     4501 ggatggaaga ggagctctcg cggttcgtct ctaaatacgg cgagagcgtg aagggttttg
     4561 gtgctagcga ttgcaatgtg gagtcgaacc tttacagaat agaactggaa gccgtttcgg
     4621 aagtattcta tgcgatagta aaaaactgga gggagagaga tgatagcttt tttgactgac
     4681 tggggatacg acagttacta cgtcgggata gccaaggccg tggtaaggga gataaacagg
     4741 gacgtggaga ttctcgacat ctgccacaat ataaagcctt tcagcgttag gcacgcagct
     4801 cacgtgatag agagggcgat caaagacctg ccggccgata cggtcttcct ggccgtggtc
     4861 gatcctggtg tagggacttc tagaaagcct gtggcgatgg ttctcaagaa tggaatgtgc
     4921 atggtcggac ctgacaacgg gatcttcacg ctggccgccg agagttttgg cgtccacgaa
     4981 gtgagggagc tcgagaaccg ccaataccac aggggagttt caaggagctt ccacggaaga
     5041 gatatcttcg caccggtcgc cgcccacata gccaagggac tggcggtgga gaagctggga
     5101 tccagactga tgaacttcga atacctcaaa tacagaaagg caaccagaag cggagacgct
     5161 ctcactggcg aggtggcctt ctacgacggt ttcggcaatc tcgagaccaa cattccgctc
     5221 tctctcggaa cagggctgga agagggggat attctggagt tgagccgtgg tagaaagcag
     5281 ttcactataa ccttcggcaa cacttattac gacgtgaatt tcggacagct tatggcccac
     5341 ttcgattcca gcggttatat ggagataacg gtcaacaagg gaagcgccag ggaactcctc
     5401 ggaatggacg aaggagagca gataacggtt gtcaaagcca gataaggtcc ttttcggttc
     5461 cggttaccac tacatcacgg gaatcgtggt ggcgggtgcc ggcttgctgg cctcgttgct
     5521 tatcgggttc aacgtggaga gtctcctgct ggttatcttt tcaggtcttt cggcgacggc
     5581 cttcgtttac ctggtgatcg tgagacagct caacaggctg gtgaaactca tacggagcgg
     5641 cgagcggaaa ccggctgccg aactggaggg ccagttccac gaactttacg gtttttacag
     5701 gaacctctcg cttcaggtgg agcaggagag aaactactac gaaaagctat acagggatta
     5761 ttccgagctg ctgaacacgc tgaacatatc cgtggtttcg ctcgatggga gcggaaatat
     5821 tgatctggcg aacgaggctt tcgtaaagag cttttcctac ggcgcccaga acatgagata
     5881 cctgaagctc gatcatttca taagaagaac cgggctgctc tttccactcc aggaggggca
     5941 gtacgatgtt tacagtaaaa aactgggcag gcgttacctg gtgacggtga ccaaaaagaa
     6001 gaggagcggc tttctcgtca ctctggatga caagactagc cagtggaaga ccaggcaact
     6061 cctcgagaag accaggcgct acgccgtcga tgcccagacc gtcgccgacc tggcccacgg
     6121 gctcaaacag ccgctggcca acatgaggct ggcgctggac ctgtatatac gctccaggaa
     6181 agactcctac ctgcaaacac tctcgaacga gctttcgggt ttccaggtga aggtgggcgg
     6241 aattttgcaa atcttcaggt atggcgagga gttcgaacgg ctcgatctct ccgaatcggt
     6301 caggaaggtt ctttcgttca tggaaccgat actggcagcc agaagtatat ctctcgatac
     6361 aatctcgctg gatgacggaa aggtcctggt tcaaaagggt aggctggaga acgtagtgaa
     6421 aaacctactg atgaatgcgg tggaggccag cgaagacgga ggggagatcg ctctgaaggt
     6481 tcgccgttcg agcgagtacc tcactctgat cgtgtccgac cggggaaagg gcatcgacga
     6541 gtcgatacag gccgaggtct tcaaaccctt ctacacgacc aaatcggagg gttcggggct
     6601 gggactgtac ctgatcaaga atttctgcga tgagaacgat gtaagattga agatgaaaag
     6661 cgttccggga aagggaacgg ttttcgtttt gactttcagg aggaatgatg atgaatcgca
     6721 ggattcttct ggtagatgac gatctggcct tcaacggcct tctcgcggcg gctcttcgcg
     6781 aggaaggtta cgatgtgaca caggtttaca atctgaagca agccgaaaag tcgctgctgg
     6841 acgagttctt cgattgcgtg atactggatg tgagacttcc agatgggtca ggattggacc
     6901 ttctcccaga tgccgcccag accgctccgg tcatagttgt gtcggcccac ggcgatataa
     6961 acaccgccat agacgcggtg agaaagggag ccttcaactt cctggagaaa cccttcgatc
     7021 tgacccacgc cctgctggag gtgaaaaggt cgatagagta ctcggaactc agcagggaga
     7081 gagacgatct ggcaggaatg gtcggaggcg atacgctctc ggagttcgtg ggaaagagtc
     7141 cgtctgttat tcgtctgaaa gagacgatct cgatgatcgc tcccaagaag gtaacggttc
     7201 tcctggaggg agagagtgga acgggcaagg agatagttgc acgggcgatc caccaattga
     7261 gcggacggaa gaggttcgtc gcgatcaact gcggggccat accggagaac ctcttcgaga
     7321 gtgagctgtt cggctacgaa aagggagctt tcaccggcgc gcttcattcc aggcccggcc
     7381 tcatagagga gtcgagcagg ggaactctct ttctagacga ggtttcggag atgccgctcg
     7441 cccttcaggt gaagctgctg agggtgttgg aaacctccac caaccagcgt cttggtggca
     7501 acatctcgcg caggctggac ctgagggtca tagcggctac caacagggac ctggaatctt
     7561 acgtgactgc cgggcagttc aggagcgacc tgtactacag gttgaacgtg gtgaagttga
     7621 agatcgctcc gctcagggag aaacgggagg acattcccgt gctggtcgag tactttctcc
     7681 ccaggctctg ccaggagctc ggtctgagaa aaaaccccat gataccggcc ggtttgctgg
     7741 acgagatgat ggggtacgac tggccgggca acatccgtga gctcagaaat aagctcctct
     7801 ccatgcttgc cgttaacgga gacagagaga tcctctcctc gataggtctg gaaaatcctg
     7861 aaaacgaaaa gtgcgtcgaa aaaggtttcg aagaggtatc actggatgag atggagagac
     7921 gctatatctc gtggttgttg aagagacact cgggaaacaa gagcgagacg gccagggtac
     7981 tgaagataag caagagtacg ttatacgaga aaatgaagag gtgggaaagc tttgaaggtg
     8041 ataacgaccc atcttaaccc cgatt
//
LOCUS       DAOOLY010000102         7975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10640_length_7975_cov_5.078409, whole genome shotgun sequence.
ACCESSION   DAOOLY010000102 DAOOLY010000000
VERSION     DAOOLY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10640_length_7975_cov_5.078409"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..457)
                     /locus_tag="PLD15_06500"
     CDS             complement(23..457)
                     /locus_tag="PLD15_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPB63590.1"
                     /translation="MRELAIVKELKRDTVLLEKARTQACSSCGSKNSCSVSQSDKIVT
                     IRALRNDVEVEPGDTVEIETGKLSATRVAMIVYGIPLVVFLTVLIVINTFLKSEGLAV
                     GLAFASVAVVYGIISIYDRRNRQKLMPRIIRKVVLPDGFITQ"
     gene            complement(471..980)
                     /locus_tag="PLD15_06505"
     CDS             complement(471..980)
                     /locus_tag="PLD15_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63591.1"
                     /translation="MVKKYLMAVLLVVSILCTGTPSIIIDGEHFASIGKETPSLVYDG
                     ISVAYLLDSGGKNSLELGGGFSFVSPVSSRDYFQNDFFNTVLKVFVGQAYDFTGESSA
                     GLRVVSRVGFLVLNFGKFGFFYSIAPVFYVRVGFLSFGVSVGLEIEQFFGEKTLPVFH
                     TGGEITYRF"
     gene            complement(1042..1869)
                     /gene="amrB"
                     /locus_tag="PLD15_06510"
     CDS             complement(1042..1869)
                     /gene="amrB"
                     /locus_tag="PLD15_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPB63592.1"
                     /translation="MTRKPVVSGKFYAGDSEDLKIQIKNCFLHPAGPGTLPVGRGQKR
                     GLSGLIVPHAGYMASGPVAAWGYYEASLLVQPAVVVIVGPNHTGLGTRLSLWTDGAWS
                     TPFGEVDIDEDLCRKLIENSNGLIQADTSAHLYEHSIEVQLPFLQYVYSQISLVPIVM
                     TDQRLELALILADIIKKSIGDREDMLIIASSDLNHYESHEKTMKKDSRLVQLLVEGKY
                     EEAYSLTREERISACGLGPMVAVRSLFTEMNVLKNTSSGEIIGDRYRTVGYLASILR"
     gene            complement(1866..7955)
                     /locus_tag="PLD15_06515"
     CDS             complement(1866..7955)
                     /locus_tag="PLD15_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPB63593.1"
                     /translation="MLRRDVESPLASREYEYTILEAGSQILETVRQVLEEMKIDVTVI
                     GYGNNMIIVGYREALDSAVNIISSISDRTDAIVVDEKLKYVFTEIPADKMDEFSSILD
                     NLKLPVKLLSSPSGVILVGEELETDKALEVVQSILSKSEIDETVESRLFNNLAGWEEG
                     KLTTYLRSYLGEKDWVKISITSIQSGYLIVGPSGVLDRIQKELSRLTGLEKPHYRIID
                     SLPTVQNLELLFERMGLSITVVITDGKTMLIGPERDVLQALKVLDELKEDVEITQTGE
                     EILFTFIDIPTEEIENFKAIFERLAITVDILGTPTGTIVVGVQGAIDRARETATSILS
                     RREAQIVPGEQSYLTLEIRDGFDLAAAQSVISAFNLNVYPLSVAGKLVMIGAQSELER
                     FLTIRNDIVDEHRITVIVPREVTLEELNGIKDTLKLDVSVFEMRSNFVVYGKEQDISS
                     LRSILNEVVVKQPVEETPQIEMLTNVGVDLDYLREILSSMGITIKVYGEGDSIVAVGK
                     PSDLNSLKNLLKKIGSASTATEPTVSFYPLLEGWTGDILSSFLQAMNVDVLVFQENRQ
                     GYLLIGVREDLDRVKVLLDGLEIKIKKVTELYAIPSGMSFEELRDILLSSGFNLSYTR
                     LGQSMSVSGPEEDVKMAIAMLDEVIRSGLGKVLSYTLIDLPTELTLEKFQKIMTDMGL
                     SVSTVQIDSRLMLIGSDSDLRKAGDIVEYLRPEEVLEDSEKIYSVVALPEALTIEETV
                     LLAERLGLNVEILPVAGNMVVIGTRSSVDSFKSLVTTISGALGAGSKETVSYRISSLP
                     AGVGLTEFESLLDSLKISSRIVQVSDSIVFVGTGEENALAEKLLSEFRPEEVPVQDVT
                     REYTTILMPPGFDLASLSGILEKIGLEADLTLAGNVLVLVGDAQSLPKVVSVISDLHG
                     TGSRPEVMKLAYEVIDVPADMNIDVVNEAINSLKIPSTIIRNGDKLLVTGSSADIEET
                     RRLVEIFRPEQSAEPVEKTYRLLSLPSGFTVFDVETALGKTGIELELLQVGNVLLLAG
                     NAESLDEAESLLEKLIMVSTGDTGERNTYEIVKIREGVTTQLLGQLFRLIGISVEILE
                     SEDWMVFTGSEGSVKAALDVFESLAKDVGGTEEPLSYTITILPKNLTIGQLQTAFESI
                     QISIRLIEAGDYAIMVGTKDSLNKGQQLISSLRIGVDASGTSTDGSISYVTFAIPVGT
                     EIDQLETIAGLMELNLKFQIVGDRVFMIGTEKDMRSFEEILKGLVVEETGVINQFRFV
                     KAITGIDSVTLDMYLTAKGIELAGIYDVSGGYLLVGDSESIDLAQVAVNYIADNQRVH
                     FDYIDLPENFDPGILQKMAEDLLLGVSLIQVSPSRYMLIGAAEDVQNLITVIFQAAGT
                     GEKLLDYSMVVLSPTLEKELDLDAFRNILAEIGIKVSVGRFSGRLIFVGKPDAIEAST
                     NLLEKLRSSMEEDGIGKLAFYELPVVGGWSIADIQNYLEAAGIKLSSVIEYAGRIVGI
                     GTQNDLAMAEEALLFIASKAARESVRIDKGLVTQIQLSEMISKLNLKAEFVELDKQWL
                     LIGDPDSLMKLTRTIEEAAADREISRYVTDLTVDPQELASLLREAIDGLTVQTFENLQ
                     MILLKSKSSVVLDNAEAMIKSIQESRKAVDPVEKGVTVSGSKVGINVVNQDLERLLRV
                     VAEKLDIPLLFIDVIDENITMSVKEIEWESLVKVINATKPVSISRIDDIYAVTKKEQK
                     SGQEATDIELVYRVYHNVEEVTKLIEFYGGEVLSDAVNGYIVVRGLAKSKVDSIFEEI
                     ASSLAKPKKQVRIETKLVDKSLVDEMQRDFNTAIGITNPDVIIQNGSIDLSFKVFENL
                     DISKILNSIVNTANASIGADLRDRDVDSDLISSPSIVSMSGEEATIHIGDTIPYLVKK
                     IEYVDGRPVEIETIENLNTGVELRITPTVNDDGKILLDLYIKVSEPEKYVDGTRTLYG
                     EKTREAKSKLVIGDGNTLTIGGLVANKDSVNVNKMPFLSDLPFIGKLFTTETKTTDKR
                     ELVIFITAEVVQP"
BASE COUNT         1851 a         2170 c         1808 g         2146 t
ORIGIN      
        1 ctccagaagg cgaagagcca cattactgcg tgatgaatcc atctggcaag accacctttc
       61 tgattattcg cggcatcaac ttctgtcggt tgcgcctgtc gtatatcgat atgatgccgt
      121 aaaccacggc gacggaagcg aaggccagcc cgacggccag tccctcggat ttcaggaatg
      181 tgttaataac tatcaggacg gtgagaaaaa cgaccagcgg aatgccgtac actatcatgg
      241 ccaccctggt ggcggagagc ttgccggttt ctatctcaac tgtatcgccc ggttcaactt
      301 cgacatcgtt tctcaatgcc cttatagtga ctattttatc gctctgtgaa acactgcagc
      361 tgtttttgga gccgcagctc gagcaggcct gagtgcgagc cttttcgagg agcacggtat
      421 cccttttcag ctccttcaca atcgccaact ctctcatgct tgatcctccg ttagaaccgg
      481 taagttatct caccaccggt gtggaatact ggaagcgtct tctctccgaa gaattgttcg
      541 atctccagtc ccacgctcac accgaacgac aggaaaccga cacgtacata gaaaacggga
      601 gctatggagt agaaaaaacc gaacttgccg aagtttaaga ccagaaaacc gacccttgac
      661 acgactctca aaccggccga cgattcgccg gtgaaatcat aggcctgacc gacaaagacc
      721 ttcagaaccg tattgaaaaa atcgttctgg aagtaatccc tggaagagac cggtgaaacg
      781 aaagaaaaac cccctccgag ttccagggaa ttcttgccac cggaatcgag aaggtaggcc
      841 acgcttatac catcgtacac tagtgatgga gtctctttgc ctatactggc gaaatgttcg
      901 ccatctatta ttatcgatgg agtgccggta cagagaatcg ataccaccag aagcacagcc
      961 atcagatatt tcttgaccat cgatcacacc tccagctcac aaaattatag catggtggtt
     1021 actccgtcgc gagaagagga attatctgag gatactcgcc agataaccga ccgtacggta
     1081 cctgtcgcct ataatttcgc cgctggaagt gtttttcaga acattcattt ccgtgaacag
     1141 actcctcacc gcaaccatcg gtcccagtcc gcaggcactt atccgttctt ctctggtcag
     1201 actgtaggcc tcttcgtact ttccttcgac cagaagctgg accagtctgg aatccttttt
     1261 catcgttttt tcgtgagact cgtagtggtt cagatcggag gaggctatta tcaacatatc
     1321 ctctctatcg cctatagact tctttatgat gtcagccaga atcagtgcta gctccagcct
     1381 ctgatcggtc ataacgatcg gaaccagact tatttgagaa tacacgtact ggaggaaggg
     1441 aagctgcact tctatggagt gctcgtaaag atgggccgac gtatcggcct gaataaggcc
     1501 gtttgaattt tctatcagct ttctacagag atcttcatcg atatcgactt ctccgaaagg
     1561 agttgaccag gctccatcgg tccagagaga gagtctggta ccgagtccgg tatgatttgg
     1621 acccactatc acgacaaccg ccggttgaac cagcagggag gcctcgtagt atccccaggc
     1681 agccaccggg ccgcttgcca tataaccggc atggggcact atgagaccgg ataatcccct
     1741 cttctgccct ctgccgaccg gcagcgtccc cgggccggcc ggatgcagaa agcagttctt
     1801 tatctgaatc ttcagatcct cgctgtcgcc ggcgtaaaac tttccgctga caacgggttt
     1861 tctcgtcaag gctgtaccac ctccgccgtg atgaagataa caagctctct cttatcggta
     1921 gtcttggtct cggtggtgaa aagcttaccg atgaacggca gatcactcaa gaaaggcatc
     1981 ttgttgacgt tcacggagtc tttgttggca accagtccac cgatagtcaa cgtgttgccg
     2041 tcgccaatca caagtttgct cttggcctct ctggtctttt cgccgtagag cgttctggtg
     2101 ccatcgacgt atttttcggg ttcgctgacc tttatataga gatcgagaag gatcttccca
     2161 tcgtcgttga ccgttggagt tatccgcaat tcgacaccgg tgttgaggtt ctctatggtc
     2221 tctatttcca ccggccgacc gtcgacgtac tctattttct tcaccagata aggtatggta
     2281 tctcctatat gaatggtcgc ctcttcaccg ctcatcgaaa ctatcgaggg gcttgagatg
     2341 aggtcggaat caacgtctct atcccttagg tcggcgccga tgctggcatt tgcggtgttt
     2401 actatgctgt taagtatctt cgagatatcg agattttcaa agaccttgaa actcagatcg
     2461 atagacccgt tttgaattat aacatcggga ttggtgattc ctatggccgt gttgaaatct
     2521 ctctgcatct catccaccag cgacttatcc accagtttgg tctctattct gacctgtttc
     2581 ttcggtttgg cgagcgaact ggcgatttct tcgaatatgc tatccacttt ggactttgcc
     2641 agccctctga ccactatgta accgtttacc gcatcggaaa gaacttcgcc accgtagaac
     2701 tcgatcagtt tcgtcacttc ttcgacgttg tggtaaacgc gatatacgag ttctatgtcc
     2761 gtcgcttcct gaccggactt ctgttccttc ttggttacgg cgtatatgtc gtctatcctg
     2821 cttatcgaga cgggtttcgt ggcgtttata accttaacca ggctttccca ctcgatctcc
     2881 ttgacggaca tggttatgtt ttcatcgatc acgtctatga atagcagcgg aatatctagc
     2941 ttttcggcca cgactctcaa cagtctttca agatcctggt taaccacatt gataccaacc
     3001 tttgatccgc tgaccgtaac gcccttctcg acaggatcca cagccttccg ggactcctga
     3061 atactcttga tcatcgcctc ggcgttatcg agaaccaccg aactcttgga tttaagaagt
     3121 atcatctgca ggttctcgaa ggtctgcacc gtcagaccgt ctatcgcttc cctgagcaga
     3181 gaggcgagct cctggggatc taccgtcagg tcggtaacgt atctggatat ctctctgtcg
     3241 gccgcagcct cttcgatcgt tctcgtcagt ttcataaggg aatccgggtc tccgatgagc
     3301 aaccattgtt tgtccagttc cacaaactcc gccttcaggt tcagtttgct tatcatttcc
     3361 gagagctgaa tctgtgtcac caggcctttg tcgatcctca cggattctct ggctgctttg
     3421 ctggcaatga acagaagcgc ctcctcggcc atagctaggt cgttctgagt acctatgcca
     3481 actatcctgc cagcgtattc gatcaccgaa ctcaacttta tcccggcggc ttcaaggtag
     3541 ttctgaatat ccgcgatact ccatccgcca accaccggaa gctcatagaa agcgagtttc
     3601 ccgattccat cctcttccat agagctgcgc aatttttcga gaagatttgt agaggcttcg
     3661 atcgcgtcgg gctttcctac aaatatcaat cttccgctga accttccaac cgaaaccttt
     3721 atacctatct ctgcgaggat gttccggaag gcatccagat caagttcctt ttcaagggtg
     3781 ggactaagaa caaccatact gtaatccagt agcttttcgc cggttccggc cgcctggaaa
     3841 atcacggtta tcaggttttg cacgtcttcg gcggcgccga tcagcatata gcgagatgga
     3901 cttacctgga ttagagaaac gcccagcagg agatcctcag ccattttctg tagaatcccg
     3961 ggatcgaagt tttcaggaag gtcgatgtag tcgaagtgta cacgctggtt gtcggctatg
     4021 taattcacag cgacctgtgc cagatcaatc gactctgaat cgcctaccaa aagatatcca
     4081 ccgctcacat cgtatattcc tgcgagttct attcccttgg ctgtgagata catgtcgagg
     4141 gtgactgaat ctattcctgt gatggctttg acaaacctga actggtttat cacgccggtt
     4201 tcctcaacaa ccaggccttt aaggatctcc tcgaaacttc tcatgtcctt ttccgttcct
     4261 atcatgaaaa ctctgtcgcc gacgatctgg aacttcaaat tcaattccat caaacccgcg
     4321 atcgtttcga gctggtcgat ctctgtcccg accggtatcg cgaaagtgac gtacgatatc
     4381 gatccatcgg tcgatgtgcc gctcgcgtcg acgcctatcc ggagtgagga tatcagctgc
     4441 tgccccttgt taagggagtc ttttgtacct accatgatgg cgtagtcacc ggcctctatc
     4501 agtcgaatcg atatctgtat ggactcgaag gccgtctgga gctgcccgat agttagattt
     4561 ttgggcagta tcgtgatcgt ataggaaagc ggctcttcgg taccgccgac gtctttggcc
     4621 agcgattcga agacatccag cgcggctttt acactgcctt cgctgccggt gaaaaccatc
     4681 cagtcttcac tttccaggat ttcaacagat atcccgatga gcctgaacag ctgacccagc
     4741 aactgtgtcg ttacgccttc ccttattttc acgatctcgt aggtgtttct ctcgcctgta
     4801 tcgccggtgg agaccattat cagcttttca agaagcgact cggcctcgtc aagagattcg
     4861 gcatttccgg cgagtaaaag cacattcccg acctgcagga gttctagttc gatgccggtc
     4921 tttcccagag ccgtttctac atcaaagacc gtgaatccag acggcagaga aagcaacctg
     4981 tacgttttct caacaggttc agccgactgt tcgggtctga agatctcgac gagtcttctg
     5041 gtctcttcta tgtctgcact gcttccggtc acgaggagtt tatcaccgtt cctgatgata
     5101 gtgctgggga tcttcagaga gtttatagct tcgttcacaa cgtcgatatt catgtcggcc
     5161 ggtacgtcga tgacctcata agcgagcttc ataacctcgg gcctgctacc ggtgccgtgt
     5221 aaatcgctta tcacacttac gaccttcggc agggattgtg catctcccac cagaacgagt
     5281 acgttgccgg ccagcgtcag atcggcctcc aggcctatct tttcgagaat gcccgagaga
     5341 gaggcgagat cgaaaccggg tggcatcaaa atcgtcgtat actctctggt aacatcctga
     5401 accggaactt cctcgggtct gaactcggaa agcaacttct cggccagagc gttctcttca
     5461 ccggttccta cgaagactat cgaatcggaa acctggacaa tcctggagga tatctttagt
     5521 gaatccagca aagattcgaa ctcggtcagc cctacaccgg ccggaaggga ggatatcctg
     5581 tatgagacgg tctctttgct tccggctccc agagcgcccg aaatagtcgt caccaggctc
     5641 ttgaaggaat ccacactgct cctagttcct ataactacca tgttcccggc aaccgggagt
     5701 atttccacgt tcaatccgag cctctcggcc agaagaacgg tctcttcaat cgtcaaagcc
     5761 tcgggaagtg caacgaccga gtagatcttc tcgctatctt ctaagacttc ctccggtctc
     5821 aaatactcaa cgatatcgcc cgcttttctg agatcgctgt cagaaccgat caacatcaag
     5881 cgactgtcga tctgaacggt ggagacactc aggcccatat cggtcattat cttctggaac
     5941 ttttcgaggg ttaattccgt gggcagatcg atcagagtgt aggagaggac cttccccaga
     6001 ccgctcctga tcacttcgtc gagcatcgcg atggccattt tcacgtcttc ttcaggcccc
     6061 gagacggaca tgctctgtcc gagtctggtg taggagaggt tgaacccaga agacaaaagt
     6121 atatcgcgca actcttcaaa gctcataccc gaaggaatgg cgtaaagctc cgttactttc
     6181 ttgatcttta tctccagacc gtcgagaaga accttcactc tatcaagatc ctcccgaaca
     6241 cctattaaca gataaccctg tctgttctcc tggaagacca gcacatccac attcatggcc
     6301 tgcaagaagc tgcttagaat atctcctgtc cagccctcga gaagaggata aaatgagacc
     6361 gttggttcag ttgcagtaga tgcggaaccg atctttttca acaggttctt gagggagttc
     6421 aggtcagacg gtttaccgac ggcgactata ctgtctcctt cgccgtaaac ttttatggtt
     6481 atccccatgc tcgacaggat ttctctcaaa taatccaggt caaccccgac gttcgtgagc
     6541 atctctatct gtggagtctc ttcgaccggt tgcttcacaa caacctcgtt caatatgctc
     6601 ctcagactcg atatatcctg ttcttttcca tagacgacga aattgcttct catttcgaac
     6661 accgagacgt ccagtttcag ggtatccttg ataccgttga gctcttcgag ggtgacctct
     6721 ctgggaacta tcactgttat cctgtgctca tcgacgatat cgtttctgat agtcaagaat
     6781 ctttccagtt cactctgagc acctatcatc accagctttc cggctactga aagaggatag
     6841 acgttcaggt tgaacgcgga gatcaccgac tgagccgcgg caagatcaaa accgtctctg
     6901 atttcgagcg tgaggtagct ctgctcccct ggaactattt gagcctctct tctcgacaga
     6961 atggaggttg ccgtttccct ggccctgtct atcgctccct gtacaccgac tacgatcgtt
     7021 ccggtaggag tcccaaggat gtccaccgta atagcgagtc tttcgaagat cgctttgaaa
     7081 ttctcgatct cctcggttgg aatatctatg aacgtgaaga gaatttcctc tcccgtctgt
     7141 gtgatctcaa catcttcctt cagttcatct aaaaccttca acgcctgcaa aacgtctctt
     7201 tcgggaccta tcagcatggt ctttccatcg gttatgacaa cggtgatcga gagtcccatt
     7261 ctttcgaaga gaagctccaa attttgaact gtgggaagtg aatctataat tctgtaatgg
     7321 ggtttctcca atccggtcag tctggaaagt tccttctgta tcctgtccag aacgccagat
     7381 ggaccgacta tcagataacc agattggatg gaagttatac ttatcttcac ccaatccttt
     7441 tccccgagat agcttctgag gtaagtggtc agctttcctt cttcccagcc cgccaggttg
     7501 ttgaacaacc tagattcaac cgtctcgtct atttccgatt tggaaagtat agattgaact
     7561 acttccagag ccttgtcagt ctccagctct tcaccgacca gtatcactcc cgaaggcgag
     7621 gaaagcagct tcaccgggag cttcaggttg tcgagaatac tagagaattc atccatcttg
     7681 tcggccggta tctccgtaaa tacgtacttc agtttctcat cgacgactat cgcatcggtt
     7741 ctgtcgctta tggaacttat tatatttacg gccgaatcga gagcctctct gtaacctaca
     7801 ataatcatgt tgttcccgta accgataacg gtgacatcta ttttcatctc ttcgagtacc
     7861 tgcctgacgg tttccagaat ttgagagcca gcctccagaa ttgtgtattc gtactcacgc
     7921 gaagcgagcg gtgattcaac atctcttcta agcatctgga ttagatccac cgttc
//
LOCUS       DAOOLY010000103         7892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_10807_length_7892_cov_4.193824, whole genome shotgun sequence.
ACCESSION   DAOOLY010000103 DAOOLY010000000
VERSION     DAOOLY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10807_length_7892_cov_4.193824"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..703
                     /locus_tag="PLD15_06520"
     CDS             53..703
                     /locus_tag="PLD15_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB63594.1"
                     /translation="MFPIAVIIFAYSYIALVPVIQPPVMKLLTTKKERRIVMGKQLRK
                     VSKLERIVFPVVSTIAISIIVPQSLPLIGMLMLGNLLRENGRTKRLVEASGKYISDTV
                     IILLCVSVGAKADGKIFLSVESIMVMVLGCAAFIVATASGVLFAKLMNLFSTNKVNPL
                     IGAAGVSAVPTAARVAQKVASEEDGTNFILMHAMGPNIAGVIGSAVAAGVFLSIIK"
     gene            721..945
                     /locus_tag="PLD15_06525"
     CDS             721..945
                     /locus_tag="PLD15_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB63595.1"
                     /translation="MAGKRQFRVNINHNFCKRCGICYWICPTKTITKGELGKPQVLDH
                     TTCIGCSMCENSCPDFAIDIQEVEAVIENA"
     gene            938..2092
                     /locus_tag="PLD15_06530"
     CDS             938..2092
                     /locus_tag="PLD15_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPB63596.1"
                     /translation="MREFVLLQGDEACALGAIKAGCRFFAGYPITPATEIAETMAKEL
                     PKVGGTFIQMEDEIASAAAIIGASLSGEKSMTATSGPGFSLMQEAIGYAAMTETPTVI
                     VNVQRGGPSTGMPTKTAQGDVMQARWGTHGDHQIIAIYPSNVEEVYKYIVTAFNYAEL
                     YRTPVIFLMDEILGHMRESVYLPYESEMMVVPRTGETGLAEDDIFHPFEGDDQMMATP
                     LVKMGKSRFHVSGLVHDESGFPVGTPSDVSRLLRRLDNKIRLHSDEIVIYDEFMTEDA
                     EILVLAYGSVARSAMKAVKMAREDKINVGLFKPVTIWPFPSTRLRQLLKKVSAVIVAE
                     MNLGQILFEVSRLNKRGVKLEHLGKVDGELITPQEILDSISRVKSEIMDL"
     gene            2217..2489
                     /locus_tag="PLD15_06535"
     CDS             2217..2489
                     /locus_tag="PLD15_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPB63597.1"
                     /translation="MEILKVSSKSNPNKVAGAIAGVISKNEQVELQAIGAGAVNQAVK
                     AVAIASRFLREQGTKVYMVPGFVEIQIGEEMRTGITIKIISGKEEE"
     gene            2595..3038
                     /locus_tag="PLD15_06540"
     CDS             2595..3038
                     /locus_tag="PLD15_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB63598.1"
                     /translation="MLTVNDMLSIALKIEGAGYSYYSKLAEKTTGKIKELFSHLADQE
                     RDHASRFREMLKDIENLPVTAEWEDNVGYLKSYAEISIFPKIESEEVPQNLNKAISAA
                     MEVEKDSIIFYGDLETFIPNSESLKKIIAEEKRHLIDLVKLYGTV"
     gene            3241..3405
                     /locus_tag="PLD15_06545"
     CDS             3241..3405
                     /locus_tag="PLD15_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63599.1"
                     /translation="MKPDPVIVENLALASIFIDGHHGFIMDGVINPDRHILEIDTLME
                     AVSIEKTVNE"
     gene            3486..4793
                     /locus_tag="PLD15_06550"
     CDS             3486..4793
                     /locus_tag="PLD15_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HPB63600.1"
                     /translation="MSKENSSYQQIQAQEDRSKGLNKGALLVLGLQHAFTMFGATVLV
                     PYLTGVPVNVALFTAGIGTLIFHLLTKWKVPIFLGSSFAYIAPINAVILYHANVSDKF
                     QSVPEAMAAGVSITPEMIAYATGGIMIAGLVQVGIAILIKFIGVEKFEKLFPPAVAGT
                     IIAVIGLNLAPVAIDMASSNWWIALVALGTAVVVRLYTKGFTRLIPVMCGIIVGYVVA
                     AITGNVSFAAVSEAGWIGVPGFVLPKFSLYALSVLVPVALAPTIEHFGDIFAVSAIAG
                     KKFFEDPGVHRTLAGDGIATAVAGLLGGPANTTYSENTGVLAITKVFNPVVMRIAAVF
                     AIVLSFVPKVGALIQSIPTSVMGGIEILLFGMISAVGMRTLIENRVKVDGKNLTIIAV
                     MLVIGIGGASIGIGNVKFEGIGLAALVGLILNAIFMLTKAPEE"
     gene            4876..5406
                     /locus_tag="PLD15_06555"
     CDS             4876..5406
                     /locus_tag="PLD15_06555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPB63601.1"
                     /translation="MLEGKQVRLREYRKEDVVLAHKYINDFEVKRLLVPGIPFPMRLE
                     EEEKWYESQSAFKDTYNFAIESLESGEYLGGCGINEVDWKNSVAVVGIFLGKPYWNKG
                     FGSDAMSILLGFIFDQMNINKVNLNVYSFNERAIASYVKCGFKVEGRLRQRIFRDGKY
                     HDEVIMGILRSEFEHG"
     gene            5983..7395
                     /locus_tag="PLD15_06560"
     CDS             5983..7395
                     /locus_tag="PLD15_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB63602.1"
                     /translation="MHYSLFKSYLQPAEAKEIRKTLFAMALPAIGENVLQMLLGISDT
                     AFLGHYDWRIMTAVGTANQVVFIFQAVLVALSTGSMVLISNSIGAGNRERVNSIAWHA
                     VYLSVAAGVVLSLGSLFSGQLLGLFFPSSDAFMQSNGSQYLGTIMAGFPAMSIMIVLG
                     ATLRGSGDTRSPMIVAAVANVLNVFLDYAMIYGKFGFPEMGAFGAALATVLSRIVGSA
                     IILLLLFKNSKISMSRKPARLSRWMTREIFSIGLPTSIENLGFSTGVLVFANILFIAG
                     PQAYAAHRIGIQVESLSFMPAWGMGVAITAMVGIYNGANERRKAIGSVRQGWLIALLI
                     SSIIGTTITIFPDLFISIFTNESSIVEAGRLPVRIIGLFQVVMGTDYAVTGALRGMGD
                     TSFPMKSSLVAMWFIRLPVGFLLVKYFNMGLLGAWIGMMSDMAFRTFLKFRRFYSGKW
                     EKTADAIQSRSKNLIDNESN"
BASE COUNT         2189 a         1709 c         1921 g         2073 t
ORIGIN      
        1 agtattagca gaaaggctat ccagaaaccc accaccaaga atcttacctc ctttgtttcc
       61 tattgcggtc atcatcttcg cctactctta tatagctctg gtccccgtaa tccaaccacc
      121 ggttatgaaa ctgctgacca cgaagaaaga acgaaggata gtcatgggaa aacaactgag
      181 aaaggtatct aagctcgaaa gaatcgtatt tcccgttgta tctacaatag cgatttcaat
      241 aatcgttcca cagtcgcttc cccttattgg aatgttgatg ctgggtaatc tgcttagaga
      301 gaacggcaga actaaacgtc tcgtagaggc ttcaggcaag tacatatccg atacggttat
      361 aatactcctg tgtgtttctg taggagcaaa ggccgatgga aagatattct tgtcagtgga
      421 gagtataatg gtaatggtcc tcgggtgcgc ggccttcata gtcgcaacgg cttcgggagt
      481 acttttcgcc aaactgatga acctcttctc gaccaacaag gtgaacccgt tgataggggc
      541 agcaggagta tcggccgttc caacggccgc cagagtcgct caaaaggtgg catccgaaga
      601 ggatggaacg aatttcatat tgatgcatgc catgggaccc aatatagctg gcgtcattgg
      661 ttctgcggta gccgccggag tatttctttc tatcataaag tagagcggga ggaattttaa
      721 atggcaggga aacggcaatt tcgagtgaat atcaaccaca acttctgcaa gagatgcggc
      781 atctgttact ggatctgtcc gaccaagacg ataacaaagg gagagctcgg caaacctcag
      841 gtactcgatc acacaacctg tatcggttgt tccatgtgcg agaactcctg tccggatttc
      901 gccatagaca ttcaggaggt agaggcggtg attgaaaatg cgtgagtttg tcctgcttca
      961 gggtgacgag gcctgtgcgc tcggagccat caaagccggg tgcaggtttt tcgccggata
     1021 ccccatcaca ccggctaccg agatagcgga aacgatggca aaggaacttc cgaaagtcgg
     1081 cggtactttc atacagatgg aagacgagat cgccagcgct gccgccatta taggcgcttc
     1141 gctttcaggc gaaaaatcaa tgaccgcaac aagcggacct ggattttcgc tgatgcagga
     1201 agctatcggc tacgccgcta tgaccgaaac tcccaccgtt atagtcaatg ttcagcgtgg
     1261 aggaccttcc acggggatgc ctaccaagac ggcgcaggga gatgtcatgc aagccaggtg
     1321 gggcacgcac ggcgatcacc agatcattgc gatttatcca tccaacgtcg aagaggtcta
     1381 taagtacatc gttaccgctt tcaattacgc cgagctttac agaacccccg tgatctttct
     1441 gatggacgaa atacttggcc atatgaggga gagcgtttat cttccttacg aatcggaaat
     1501 gatggtagta ccaagaacgg gtgaaacggg tctggccgaa gatgatatct tccacccctt
     1561 cgaaggcgac gatcagatga tggccactcc ccttgtaaaa atgggaaaat cgagatttca
     1621 cgtttcgggg ctggtgcacg atgaatctgg ctttccagtg ggcacaccct cggatgtttc
     1681 aaggctcctc agacgtctgg acaacaaaat ccgtttgcac agcgacgaga tagttatcta
     1741 cgacgaattc atgaccgagg atgccgagat cctggttctg gcatatggat cggtggcgcg
     1801 aagcgcaatg aaggctgtca aaatggcacg cgaagacaag ataaacgtgg ggcttttcaa
     1861 acccgttacg atctggccct ttccgagcac gaggttgaga cagctcctca aaaaagtcag
     1921 cgcagtgatc gttgccgaga tgaatctcgg tcagatactc tttgaagtgt cgagattgaa
     1981 caaacgagga gtcaagcttg agcatctggg aaaggtcgat ggcgagctca ttacgcccca
     2041 ggaaatattg gattcgatat caagggtgaa atctgaaata atggatcttt gatcgataga
     2101 tacggttctt ttctagactc cttctgcaga ataaatagaa tgttaaaact tttgcgaaat
     2161 gggtggcctc tgtggtagaa taacagcgaa aaaacccgga gaaggaggaa atcttaatgg
     2221 agatcctgaa ggtcagttct aaatcaaacc ctaacaaggt agcaggtgcc attgccggag
     2281 taatctctaa gaatgaacag gtggagcttc aggccattgg cgctggtgct gtgaaccagg
     2341 ctgtcaaagc tgtggccatc gcctcaagat tcctcagaga acagggaacg aaagtgtaca
     2401 tggttcctgg ttttgtggaa atacagattg gcgaagaaat gcggacggga atcactataa
     2461 agatcatctc aggaaaagaa gaagagtaac gataccacaa atacagaaaa gagatcgaga
     2521 gatctctttt cttcatttct aaacgattca aaaatgctag aatagtcaat gagaaattta
     2581 aaaggaggat tacgatgctt accgtaaacg atatgctttc aatcgctcta aaaatcgaag
     2641 gggccggtta ttcctactac tcgaaactag cagaaaaaac gactggaaaa attaaagaac
     2701 tcttctcaca tctggccgac caggaaaggg accacgccag caggttcaga gagatgctca
     2761 aggatatcga aaacttaccg gtaacggccg agtgggaaga caacgtgggc tatctcaagt
     2821 cttatgctga gatttcgatc ttcccgaaga tcgaatccga agaggtgcca cagaacctca
     2881 acaaggctat aagtgcagcc atggaagtcg agaaggacag cataattttc tacggcgatc
     2941 tagaaacctt catacccaac agcgagagtc tcaagaagat tatcgccgag gagaagaggc
     3001 atcttattga tctcgtgaaa ctctacggca cggtttaaga cgggaattac ttgttgcctt
     3061 ctgttcaatt tagaattagt ccctaaaaaa taacttaaca aatctctgaa accttcatcc
     3121 agtctctcct gtagtaatta ttggagagac ttttctttat aattgccata ctatcacttg
     3181 attttatact accagatttg atagaatctc ccttgataaa tctcttaagg agttgctcag
     3241 atgaaaccag atcctgttat cgtcgaaaat ctagctcttg catcgatttt catcgatggc
     3301 catcatggat tcataatgga tggggtgatc aaccccgaca ggcatatact cgaaatcgac
     3361 actcttatgg aagcggtgtc gatcgaaaaa acagtaaatg aatgacctca cggattcccc
     3421 gtgaggtttt tttttacacc ggaacaactc ttaatacaaa cccatcatcc aggaggaggg
     3481 atcttgtgag caaagaaaat tcgtcttatc aacagattca agctcaggaa gacagaagca
     3541 aaggtttgaa caaaggggct ttgctggttc taggtcttca acacgccttc accatgtttg
     3601 gcgcaaccgt actggtaccg taccttaccg gtgttccagt caatgtcgct cttttcaccg
     3661 caggaatcgg aacactgatt ttccaccttc tcacgaaatg gaaggtcccg atattcctcg
     3721 gttccagttt cgcctacata gctccgataa acgccgttat actttaccac gccaatgtct
     3781 cggacaaatt ccaatccgta ccggaggcga tggctgcagg agtttcgata acaccggaga
     3841 tgatcgctta cgcgaccgga gggatcatga tcgccggtct ggtccaggta ggtatcgcga
     3901 tattgatcaa gttcattggt gtcgaaaaat ttgaaaagct cttcccaccg gcggttgccg
     3961 gcacaataat cgcagtcata ggtctcaacc ttgctcctgt ggctatagac atggccagct
     4021 ccaactggtg gatagctcta gtggctctgg gaactgccgt tgttgtcagg ctctacacga
     4081 aaggtttcac gaggctgatt ccggttatgt gcggaatcat agttggatat gtcgtcgctg
     4141 cgatcaccgg taacgtcagt tttgccgcgg tttcggaagc aggatggata ggtgttcccg
     4201 ggttcgttct gccgaagttt tccctttacg ctctttcggt tctggttccg gtagctcttg
     4261 ctccgacgat agagcatttt ggagatatct tcgccgtttc cgctatagca ggtaagaaat
     4321 tttttgaaga tcccggagta cacagaaccc ttgccggtga cggtatagcg acagcggtgg
     4381 ccggcctcct cggaggacca gcgaacacaa catacagtga aaataccggt gttctggcca
     4441 taacgaaagt cttcaaccct gtggtgatga gaatagcggc cgtattcgcg atagttctct
     4501 catttgttcc caaggtcggc gcgttgatac agtcaatacc tacttctgtg atgggaggta
     4561 ttgaaatact tctgttcggt atgatctcgg cggttggtat gagaactctc atagaaaaca
     4621 gagttaaggt tgacggcaag aatttgacga taattgccgt gatgctcgtg atcggtatag
     4681 gcggtgcatc gatcgggatt ggaaacgtga aattcgaggg aatagggctt gcggctctgg
     4741 ttgggctaat tctgaatgct attttcatgc tgacaaaagc ccccgaagaa tgatgagaat
     4801 ttcgcaataa atataaagac gcccttgagg gcgtcttttt ttgatatact attcgaaaat
     4861 aagggggtga aggctttgct ggaaggcaaa caggtcaggc tcagagaata caggaaggaa
     4921 gacgtggttc ttgcgcacaa gtacataaac gacttcgagg tgaaaagact gcttgttccc
     4981 ggtattccct tccctatgag actggaagag gaggaaaagt ggtacgagag tcagagcgct
     5041 ttcaaagata cctacaattt tgcgatcgaa tctctggaga gtggcgagta cctgggtggc
     5101 tgtggaatca acgaagtcga ctggaagaac agtgtcgcgg tcgttggtat cttcctgggc
     5161 aaaccctact ggaacaaagg gtttggaagc gacgccatga gtatactttt ggggttcatt
     5221 ttcgatcaga tgaacataaa caaagtgaac ctcaatgttt actccttcaa cgagcgtgcc
     5281 atagcatcgt acgtcaaatg tggtttcaaa gtcgaaggaa gactgaggca gagaattttc
     5341 agggatggaa agtatcatga cgaagtcata atgggcattt tgagatcgga attcgaacac
     5401 ggttgataca tatcgtctgg actgcttctg atttggtgtt tacgtgagtt tctcaggatc
     5461 atgtctccta tagaatcata ttctctttct ggctgaaagc aaaaaaaaga gtggtgcctt
     5521 cgcaccactt taatccacat ctctaaacaa ggggttcatt tatgtcaact cgctgttgtt
     5581 gtgcaagact tcttcgactg ctgtttcact tttatcccat tcacaataaa taacataaca
     5641 tatgaaatca tatttgtcaa ctaatacggc gatataaatt gaataatttc ttgaaatgca
     5701 taataatatc accaataaag attgtttatc caggtaagtt tagtgttgaa aagcaataat
     5761 tgaaagtatt tcaatatact ccatcatatt tgcaataagt tgatacacga tttgcttaac
     5821 ttctaggtag agtgtacaat ctaaactcat gcgttctttt cgcttttttc aaaacgcatt
     5881 tttgtcaatc tagagtgaat tagttgagat aacagcactt tcaaagacat gcagtacgtg
     5941 gtaaactaaa tatagtacgt gatccttacc gtggaggaaa aaatgcacta cagtcttttc
     6001 aaatcatacc tgcagccggc ggaagccaaa gaaatccgaa agaccctttt tgcaatggct
     6061 cttccagcga taggagaaaa tgtattacag atgctcctgg gaatctcgga cactgccttt
     6121 ctaggtcact acgactggag aatcatgacg gcggtcggaa ctgccaatca ggtggtattc
     6181 atatttcagg cagttctcgt ggccctttct acagggtcca tggttctcat atccaacagt
     6241 ataggcgccg gcaacagaga gagagtgaat tcaatagcct ggcatgccgt ttatctaagc
     6301 gttgcggctg gtgtagttct ctcgctggga tcgctcttct ccggacaact actcgggctt
     6361 ttctttccat cgagcgatgc cttcatgcag agtaacggga gccagtatct cggaaccata
     6421 atggccggtt ttccggccat gagtatcatg atagttctcg gggctacact gaggggatcg
     6481 ggcgacacaa ggtcgcctat gatagttgcg gcggttgcga acgtgttgaa cgtttttctg
     6541 gactacgcca tgatatacgg caagttcggt tttcctgaaa tgggtgcttt cggcgccgca
     6601 ctcgcaactg ttttatcaag aattgtagga tcggcgataa tacttctctt gctcttcaaa
     6661 aacagcaaaa tctctatgag tcgaaagccg gcaaggctct caagatggat gaccagggaa
     6721 atcttctcga tcggactccc gacctcaata gaaaacttag gattctccac cggtgtgctg
     6781 gtctttgcaa atattctctt catcgccgga ccacaggctt acgcggccca cagaataggt
     6841 attcaggtcg agtcgctttc attcatgccg gcctggggca tgggcgttgc cataacggcg
     6901 atggtgggta tctacaacgg agccaacgaa cgaagaaaag caataggatc ggtcagacag
     6961 ggttggctga tcgcactttt gatttcttct ataataggga ctaccataac tatttttcca
     7021 gatctcttca tttcgatttt taccaacgaa agctcaatcg tagaggccgg tcgccttccc
     7081 gtgagaataa taggcctctt tcaggttgtc atgggaacag attacgctgt aaccggtgct
     7141 ttgcgtggta tgggcgatac atcctttccg atgaaatcct ctctcgttgc catgtggttc
     7201 ataaggcttc cagtggggtt cctactggtg aagtacttca acatgggcct tctaggtgcc
     7261 tggataggta tgatgtcgga catggcgttc agaacatttc tgaagttcag aagattctat
     7321 tccggtaaat gggagaagac cgccgatgct attcagagtc gttcaaagaa cttaattgat
     7381 aatgaatcca attgaaagaa gactaaacct ctccctggca acaccaacac acgggttgca
     7441 atgtttcagt tgtgaaagcg tgtaaacaga tatttcacag aaaatgagac aggttattct
     7501 cataaaaggt atataattat tatctggcgg tagtttcact ttttcggtca atggtgcgta
     7561 ataaatagtc agcttaaatt ttttttaaca catgttttga taaggattac gtaccagatc
     7621 aagtgagaga tacagactct cgttatgata atcgagtttc atcaggtttc atcgtttctc
     7681 agggcaaaat gagggaagag taaaaagcaa ggttttatgt tataaaggaa agcggagtcg
     7741 ttttgttcgc tcgttgaagg gtttcagccc atcaatatta ttcgttatgt attgttgctt
     7801 ctagtcagat gtcgatcttc tatattattt caggaggtaa aacatgaaaa aaaccctagt
     7861 cgttctgctt gcgctcctaa ttctctcgac ta
//
LOCUS       DAOOLY010000104         7782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_11027_length_7782_cov_4.169406, whole genome shotgun sequence.
ACCESSION   DAOOLY010000104 DAOOLY010000000
VERSION     DAOOLY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11027_length_7782_cov_4.169406"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLD15_06565"
     CDS             complement(<1..306)
                     /locus_tag="PLD15_06565"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492510.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HPB63603.1"
                     /translation="MEDRELRTLNHMLFMEHYTGGNAGLFMKMKRLVYSYQSPYQRID
                     IFEHPEFGMVLVLDGITMFTEADEFMYHEMLVHVPLFTHPDPKRVLIIGGGDGGSLRE
                     "
     gene            774..2435
                     /locus_tag="PLD15_06570"
     CDS             774..2435
                     /locus_tag="PLD15_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB63604.1"
                     /translation="MKSKRFDLFFILFLAAMVFFVYRLYGYQVKSNDKYVQQVENISL
                     RSTRTLSTRGSVLDRYSTPLAWDAPIYTVRKKVVFLADDLKRTVIDGFEDRDKAESFL
                     KRLEMYGSVETDLPIALAERLKEYPELEVSERIIRYYAENPGLAHVLGYLRGDGEAVM
                     GAEKQYNDLLTGTPGEKVIRVDSRGNTVAVESESSPLPGNNVVLTIDAQLSRFAYDRL
                     LATGKNGAAVVMKTNGEVLALVSVPSFNNNLFSRRISDRDWLRMNLDPSTPLINRAIS
                     PFSPGSVIKPFIAMVALAERYDPSIEVSCGGSFQYKNTSGEVQGIYRDWFLYGHGVTG
                     LSKAITVSCNVYFYQLGLELEIETMNVYGKRWGIFEPTGIDLPGEGTGLLPSPAWKKE
                     KTGEGWFPGDTILTSIGQGYLSITPLQLARMTAEIATRGAEVLPFIGRERSSERVVID
                     LDDESWDLMINAMKDVISKGGGAAESGTAYTAFRNFQWPAAGKTGTAEQGGSLPTHSW
                     FTGFTPVEEPEIVVSVFVQDGGYGSGLASEVSRDIMEFYYENRNR"
     gene            complement(2437..3069)
                     /locus_tag="PLD15_06575"
     CDS             complement(2437..3069)
                     /locus_tag="PLD15_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPB63605.1"
                     /translation="MGCTRSLRERATFEVNIKRSRFIGNCARVRDEENARAFVKEISV
                     AFPDASHSCWAYRVSAIGREVANYSDAGEPHGSAGMPILGAIDHLGLTDVTVVVTRYF
                     GGVKLGVRGLIDAYGNTALETLKKAGIVDYCRGFELKFRLGYSQWNDFTRLLSEGKDY
                     ATVSVDYAGDVSAVIVVLEENWPRVTGFLDERRIGYEKGSEMEMPFPTGD"
     gene            complement(3069..3926)
                     /locus_tag="PLD15_06580"
     CDS             complement(3069..3926)
                     /locus_tag="PLD15_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPB63606.1"
                     /translation="MRYKWIIPIAVVAILALVLLPGFFFTIDETEQAVVLRFGEIRNS
                     ITEPGLYFKTPFVDTVRKFDKRLQIYDVDAERIYSRDKKTILMDTYALWKIVDPQKFI
                     ETMKSEQIALTRIDDVVYSNVRNAFGKLDFEDIISGQRTDVLDEITKLSARDMADFGL
                     EIVSVRVKRADLPDENRNAVFNRMKSERIQEASLIRAEGDREARKLTAEADKQAQILI
                     AEAQKEADIIIGTGDASALTVYAEAYNQDPEFYEFMKRLEVYEGTLIDGKYILGPSME
                     FIDKLLRGE"
     gene            complement(3926..4909)
                     /gene="hflK"
                     /locus_tag="PLD15_06585"
     CDS             complement(3926..4909)
                     /gene="hflK"
                     /locus_tag="PLD15_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPB63607.1"
                     /translation="MVDVRIEEPDDGFSGSVTHNRGKNFRWTGLISVLVILAVVAIYF
                     LSGFFLVGPDQVGLIKRFGKYVRTVGPGLGYHLPYPIESAVVVDTSNLRKQEIGFRTV
                     RTGTYQSVANESLMLTGDGNIVSVELVIQYYIGEPEKYAFNIVSDSDIVKFTTESVLR
                     EEVAANTIDAILTTERDAISLKTAARVQAELDNLKTGIIVKNVFLQEVAPPQQVITAF
                     DDVNSAKQDKEKLIYEAEKYRNDLIPKAEGEAAQLTRVAEGYAQERILKAEGEAEKFL
                     AILKEYSKAPEVTRTRLYLETLNKILKGNDKYIVLDDSGILKLLDLEGSGK"
     gene            complement(4909..5826)
                     /gene="murB"
                     /locus_tag="PLD15_06590"
     CDS             complement(4909..5826)
                     /gene="murB"
                     /locus_tag="PLD15_06590"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPB63608.1"
                     /translation="MAKRDLRKYFGDLYMQGADIRLEEPMSWHTTMRIGGRVPFFVSP
                     YSVDSMIKILKLVESEKIPHRLIGGGSKVVCPEHYGGIVLSTAHLNAVRIDGTSVTAQ
                     AGVPVNTLIWLCLSEGLTGLEFLTGLPGTVGGAVYMNAGAFGGEIGSLVKSVEYIDEN
                     YDLHSIEGSLCRFGYRHSLFKERGYKIMNVEFSLVSGRREAIESKMSEILRKRLEKQP
                     LDFPSAGSVFVRPYQDFFVGSTIEKLGLKTLSVGDAQVSSKHAGFIVNRGKASYTDVV
                     LLIEKLKESVLEATGVTLSTEIELWREVE"
     gene            complement(5808..6422)
                     /locus_tag="PLD15_06595"
     CDS             complement(5808..6422)
                     /locus_tag="PLD15_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63609.1"
                     /translation="MERQELIDRIVEEKGEEAVPLLLDLMVSEDDETAQICFEALLQL
                     GGRTSPELLKRLREGGEDISILYLADLAGELGDKKAVPYLYDLLRRFDDERSQAVIYE
                     ALARLGEGSKVAGILSLLLQESDDESSRDQLIMAISLTASPEGVKTLARMYYDTKFDK
                     STRAFVLEGLHMLLSSNAKLKDVLLELDNGREIMERLYIWQKEI"
     gene            complement(6404..>7782)
                     /locus_tag="PLD15_06600"
     CDS             complement(6404..>7782)
                     /locus_tag="PLD15_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPB63610.1"
                     /translation="IEIEDGNQLLVSVNFTGQRTIYRLNGRMVPRQMIQILFKDLIEI
                     HSQHSSVALLDSNRHYQILDHALQGEKSLEEYREFYDRYSSIKRELDSLVMDPSQIER
                     EKDFLVFEINEIEQAELKPDEDGLLETRYRKYKNGKFLVQTFRELMDRLKDGDESVYN
                     SLNDILSLVTKVESFGYSRWLSHVQLALEELEGLYNLVGEESDGLEMDEEEFRAIEAR
                     ISLIQGLKRKYGRTVSDILQSCEEFKSKLKSLQELQKLEEKLRKEESIYLQKLREVGR
                     VLDGVREKRAIEMARDIRVHLEDLKMKGAELEFRLFQEEEPKYYGTSRVSIMVKTNPG
                     MEFMEIGRVASGGELSRCLLALESTLKDQLDIRTIVFDEVDSGVGQRLGQVVAEKLLE
                     ISSKVQSIVITHLPQIASRAERHFAVRKEQSELDTISTIEELSGEKRERELVEMSGLR
                     GEEWKGRN"
BASE COUNT         1765 a         2146 c         1835 g         2036 t
ORIGIN      
        1 ttctctcaag ctcccaccgt cgcctccgcc tattatcaaa actcttttgg gatcggggtg
       61 ggtgaacaga ggaacatgaa ccagcatttc atggtacatg aattcatcgg cttcggtgaa
      121 catggttatc ccgtcgagaa ccaggaccat cccgaactcg ggatgctcga agatatctat
      181 tctctggtaa gggctctggt atgagtaaac gagtcgcttc attttcataa acagaccggc
      241 atttccaccg gtatagtgtt ccataaagag catgtggttc aaggttctca gttcacggtc
      301 ttccatttta atctacacct ccaggtcgta tataataatt attcaattcc tctccaagga
      361 tataatacat ccacgcgggc aaaatcatct aatgttatca gacccgagct tacgctttgc
      421 gcgaatgcgg aatactcttc aaatctggac agcaaagtag attctatcac tttgcaggaa
      481 ggatagtcaa atttcctggc ctccgatttt cgcgccgctg cgacttttcc tgccttaggc
      541 aggaagtcgg ctttttctcc cggtcaaagg atggtaaaat tgtaaacgaa cgattctttc
      601 tcgcaggagg attcaggtta tgttcttcag aaaggattcg tgtatcgatc aattcttacg
      661 ccaggaaccg cgatatcggc caaaatgaca gtttcaatac aagggttctc acccctgccc
      721 tacggcagtc gcgatgcaaa gtttcgacaa cgacgaccga ggaggtcgag tatttgaaga
      781 gcaaaaggtt cgatcttttt ttcatcctct tcctggccgc aatggtcttt ttcgtttaca
      841 ggctttacgg ttaccaggtg aagagtaacg ataaatatgt tcagcaagtc gagaacataa
      901 gtcttaggtc cactagaact ctatccaccc gcggcagtgt attggatagg tactctacac
      961 cgctggcctg ggatgcacct atttacaccg tcaggaagaa ggttgttttt ctggccgacg
     1021 atctgaagcg tacggttatc gatggctttg aagacagaga caaagccgaa tcctttctca
     1081 agaggctgga gatgtacggc agcgtcgaaa ccgatctccc gatcgcgctt gccgaacgtt
     1141 tgaaagagta tcccgagctc gaagtgagcg agaggatcat aaggtattac gccgaaaacc
     1201 ccggattggc gcatgtattg ggttacctgc gaggagacgg agaagcggtc atgggagccg
     1261 agaagcagta caacgatctg cttacaggaa ctcccggaga aaaagtgatc agggtagata
     1321 gcaggggaaa caccgtggcg gtcgagagcg aaagctcccc ccttccggga aataacgtcg
     1381 tgctgacgat agacgcccaa ctgagcaggt tcgcttacga tagactgctc gccacaggga
     1441 agaacggagc cgccgtagtt atgaagacca acggagaggt tctggctctg gtctcggttc
     1501 ccagtttcaa caacaacctc ttctccagaa ggatctctga tagagactgg ttgaggatga
     1561 acctcgatcc atcaacacca ctgataaaca gggcgatcag ccccttctca cccggttcag
     1621 tgatcaagcc ctttatagcc atggtggccc tggccgaaag gtacgatcct tcgatcgaag
     1681 tgagctgcgg ggggagtttc cagtacaaga ataccagcgg cgaagttcag ggaatttaca
     1741 gagactggtt cctgtatggc cacggtgtaa cgggcctgtc gaaggccata accgtctcct
     1801 gcaacgttta cttctaccag ctcggtctcg agctcgagat agagactatg aacgtttacg
     1861 gcaaaagatg gggaatcttc gaacctacgg gtatagatct cccgggagag ggaaccgggt
     1921 tgctcccttc tccggcctgg aaaaaggaaa agactggtga aggctggttt cccggtgata
     1981 ctatcctgac ttctatcggt cagggatatc tcagcatcac tccgctccag ctggcaagga
     2041 tgacggccga gatagccacc aggggcgccg aggttctacc gttcatcggc cgggaaagat
     2101 cgagcgagag agttgtgatc gacctggatg acgagagctg ggatctcatg atcaacgcga
     2161 tgaaggatgt tataagcaaa ggcggcggcg ccgccgaaag tggaacggca tacaccgcct
     2221 tcaggaactt ccagtggccc gcggccggca agacaggaac ggccgagcag ggaggttcgc
     2281 tgcctaccca ctcctggttc acaggattta cgcccgtaga agaacccgag atagttgttt
     2341 ccgttttcgt tcaggatggc ggttacggtt ctggactggc ctccgaagta tcgagggata
     2401 tcatggagtt ctactacgaa aaccggaacc gataggtcaa tctccggtcg gaaaaggcat
     2461 ttccatctcc gatcctttct cgtatccaat ccttctttcg tccaggaagc ccgtaactct
     2521 gggccagttt tcctcgagca ctacgatgac agcagagaca tcaccggcgt aatctacgct
     2581 cacagtggcg taatctttcc cttcactcaa aaggcgggtg aaatcgttcc actggctata
     2641 accgagcctg aatttcagtt cgaaacccct gcaataatcg actattcccg cctttttaag
     2701 cgtttcgaga gccgtgttgc cataagcgtc tatcaaaccc cttactccga gcttaacccc
     2761 gccgaagtaa cgtgtcacga caacggtcac atctgtcaat ccaaggtgat cgatagcccc
     2821 cagtatcggc atgccggccg aaccgtgagg ctctccggcg tcggaatagt tggccacttc
     2881 ccttccgatg gccgagaccc tgtaagccca gcaactgtgg ctggcatcgg gaaaggccac
     2941 cgatatctcc ttcacgaagg cccgggcgtt ttcttcgtct cttacccggg cgcagttacc
     3001 tatgaatctc gatcttttaa tattgacctc gaacgtggcc ctctctctca acgatctggt
     3061 acagcccatt actcccctct caaaagcttg tctatgaact ccatcgaagg accgaggatg
     3121 tatttgccat cgataagcgt accctcgtaa acttcgagcc ttttcatgaa ttcgtagaac
     3181 tcgggatcct ggttataggc ttcggcatat acagtgagag cggaggcgtc tccagtaccg
     3241 ataattatgt cggcttcctt ctgagcttcg gcaattagta tctgtgcttg cttatcagcc
     3301 tcagccgtca attttctggc ttctctgtcg ccttcggccc ttatgagcga agcctcctgt
     3361 attctctccg atttcatacg gttgaagacg gcgttcctgt tttcatcggg caggtcggcc
     3421 cttttcaccc ttaccgagac tatctccagg ccaaagtcgg ccatatccct ggcggaaagc
     3481 ttcgttatct cgtccagaac gtccgtcctc tggcccgaga ttatatcttc gaaatcgagt
     3541 ttgccgaagg cgtttctgac gttcgaatag acgacgtcgt ctattctggt gagggctatc
     3601 tgctccgact tcatcgtctc gatgaacttc tggggatcta ctatcttcca tagcgcatag
     3661 gtatccatga gaatcgtctt cttgtctctt gagtagatcc tttcggcatc gacgtcgtat
     3721 atctgaagcc tcttgtcgaa ctttctgacc gtgtcaacga agggggtctt aaagtacagc
     3781 cccggttcgg taatggagtt gcggatctct ccaaacctca gcacaacggc ctgttcggtt
     3841 tcatctatgg tgaagaagaa gcccggcagc aagaccaggg cgagaatcgc aacgaccgcg
     3901 atcggtataa tccacttgta cctcatcatt taccactccc ttccagatca aggagtttca
     3961 aaatcccgct gtcatcgagg actatgtatt tatcgttgcc tttgagtatc ttgttgagtg
     4021 tctcaagata caacctggtc cgcgtcactt ccggcgcctt cgaatactcc ttcaggatcg
     4081 ccaggaactt ctcggcctcg ccttcggcct tgagtatcct ctcctgagcg tagccctcgg
     4141 cgactctggt gagttgggcc gcttctccct cggctttggg gatgaggtcg ttcctgtatt
     4201 tctcggcctc atagatcagc ttttctttat cctgtttggc actgttaaca tcgtcgaagg
     4261 cggttatcac ctgctgtggc ggggccacct cctgtagaaa gacgttcttg acaataatac
     4321 cggtcttaag attgtccagt tccgcctgga ccctggcggc cgttttcaag gatatcgcat
     4381 ccctttcggt ggtgagtatg gcgtcgatag tgttggccgc gacttcttcc ctgagtacag
     4441 attcagtcgt gaacttgact atatcgctat cactgacgat gttgaaggcg tacttctccg
     4501 gctctcctat gtagtactgt atcaccagtt ccaccgaaac gatatttccg tcacccgtaa
     4561 gcatcaacga ttcgttcgcc acagactggt aagtaccggt cctgactgtt ctgaaaccta
     4621 tctcctgttt tcgaaggtta gaggtatcca ccaccacggc gctctctata gggtagggca
     4681 gatgataacc cagtcccggc cccaccgtac ggacatactt gccgaacctc tttatgagcc
     4741 cgacctgatc gggtcccacc aggaagaaac cgctcaggaa gtaaatcgca accaccgcca
     4801 gtattaccag tacgcttatc aatccggtcc agcggaagtt tttccctcta ttgtgtgtca
     4861 cagatccgga aaacccgtcg tccggctctt cgattctcac atcgaccatt attcaacctc
     4921 cctccacaat tctatctcgg tactgagagt cactccggtc gcttcgagca cagattcctt
     4981 tagcttttca attagtagta cgacatcggt gtaactcgcc tttcccctgt tgactatgaa
     5041 gccggcgtgc ttgctagaaa cctgcgcatc gccgaccgaa agcgttttca agcccagctt
     5101 ctcgatggtc gaacccacga agaagtcctg atagggtctt acgaaaacgc tcccagcgct
     5161 cggaaaatcc aggggctgtt tttcgagacg cttcctcaat atctcgctca tcttgctctc
     5221 tatagcttct cttcgaccgc taacaagcga gaattcgacg ttcattattt tgtagccccg
     5281 ttctttgaag agactgtgcc tgtatccgaa tcggcataaa gaaccctcta tcgaatgtaa
     5341 atcgtagttt tcatctatgt actcaacaga tttcaccaga gaacctatct caccaccgaa
     5401 agctccggcg ttcatgtaaa cggcgccacc cactgtaccg ggaagaccag tcagaaactc
     5461 cagaccggta aggccttccg aaaggcagag ccagatgaga gtgtttaccg gtacaccggc
     5521 ctgtgccgtg acggaggtgc cgtcgatcct gacggcattt aggtgagcgg tagatagaac
     5581 tattcccccg taatgttccg gacagacaac cttcgaacct ccacctatga gcctgtgtgg
     5641 tatcttctcg ctttccacca gtttcaggat ctttatcatg gaatcgacgg aatagggact
     5701 cacgaaaaac gggactcttc cccctatcct catggtggtg tgccagctca taggctcttc
     5761 taacctgata tcggcacctt gcatgtacag atcgccgaag tattttctca gatctctttt
     5821 tgccatatat agagcctttc cattatttcc cttccgttgt cgagttctag gagaacatct
     5881 ttcaatttgg cgtttgaaga gagcaacata tggagacctt cgagcacgaa ggccctggtg
     5941 gatttgtcga attttgtgtc gtaatacatc cgggccagcg ttttcacccc ttccggagaa
     6001 gcggtcaggc tgatagccat gatcagctgg tctctggaac tttcatcgtc gctctcctgc
     6061 aaaagcagcg aaaggatacc ggccaccttc gaaccttcgc ccagccgggc gagagcttcg
     6121 tatattaccg cctgcgatcg ctcgtcatcg aagcgtctca gtagatcgta aaggtaaggc
     6181 acggcctttt tgtctcccag ttcccctgcc agatcggcca gatagaggat agagatatcc
     6241 tcgccaccct ctctcagtct cttcaacaat tccggagaag tcctcccacc cagctggagg
     6301 agtgcctcga aacagatctg cgctgtttca tcgtcttcgc tcaccataag atccaggaga
     6361 agcggtacgg cttcttctcc cttttcctca actattctat ctatcagttc ctgcctttcc
     6421 actcttcacc tctcaagcca ctcatttcaa cgagctctct ctcccttttt tctccggaga
     6481 gttcctctat tgtagatatt gtatcaagtt cagattgttc tttccttacg gcgaagtgcc
     6541 tttctgctct cgaagctatc tggggaaggt gtgtgattac tatcgattgc actttcgaag
     6601 agatctccag cagcttctcg gccaccacct ggcccagacg ctgccccact ccggaatcga
     6661 cttcgtcgaa gacgatggtt cttatgtcaa gctgatcttt aagtgtcgat tcgagcgcca
     6721 gcagacacct ggaaagctca cccccggagg ccaccctgcc gatctccatg aactccatac
     6781 cggggttcgt cttgaccatg atagagaccc tggaggtacc gtagtattta ggctcctcct
     6841 cctggaacag tcgaaactcg agttcggcgc ccttcatttt caagtcctct agatgaacgc
     6901 ggatgtcccg ggccatctcg atcgcccttt tctctcttac gccatccaga actcttccga
     6961 cctccctcag cttctgaagg tagatcgact cctcttttcg taatttctct tcaagctttt
     7021 gaagctcctg gagcgacttg agtttgcttt tgaactcttc gcagctttgg agtatatcgc
     7081 tcaccgtccg gccgtacttc ctttttagcc cctgaatcag ggagatgcgc gcttcgattg
     7141 ctctgaactc ttcctcgtcc atctcgagac cgtcgctttc ttctccaacg agattgtaga
     7201 gtccctccag ttcctccagt gccagctgaa catgtgacag ccaccgggag tatccgaagc
     7261 tctcgacctt cgtgaccagc gacaggatat cgttcagcga attgtaaaca gactcgtcgc
     7321 catccttcag acgatccatg agctcccgga aggtttgaac caggaacttc ccgtttttgt
     7381 atttcctgta ccttgtctcg agcaggccgt cctcgtcagg tttcagttca gcctgctcta
     7441 tctcgtttat ttcgaaaaca aggaagtcct tctctctttc gatctgagaa ggatccatca
     7501 cgagcgaatc cagctctctc tttatactgg aatacctgtc gtaaaactcc ctgtactcct
     7561 ccagagactt ctcgccctgg agggcgtgat cgagtatctg atagtgtctg ttcgaatcca
     7621 gcagggctac gctgctgtgc tgcgaatgga tctctataag gtctttgaaa agaatctgga
     7681 tcatctgcct gggcaccatt ctgccgttca atctgtatat agttctctga ccggtgaaat
     7741 tgacgctcac caacagttga ttaccgtctt cgatctctat tg
//
LOCUS       DAOOLY010000105         7756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_11079_length_7756_cov_4.375276, whole genome shotgun sequence.
ACCESSION   DAOOLY010000105 DAOOLY010000000
VERSION     DAOOLY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11079_length_7756_cov_4.375276"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLD15_06605"
     CDS             complement(<1..957)
                     /locus_tag="PLD15_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63611.1"
                     /translation="MKVYFLKRFLELIPIFFAISIIIFVIMNSMPGDPLLQMRMQNPR
                     AMANDPQRMKELREYYRLDDPLPLKYFTWLKSVLTGDLGYSSMYKTPVIDLIASRLPN
                     TLILTITAWLIGLVVALPIGILSAVKKYSTFDYTTTVFAFIGISLPSFWFALIAIIIF
                     SVNLKWLPVSGMATYGVTGFWNIFVDRLRYLILPAAILGLVQVASWVRYIRTSLLEVL
                     DQDYVRTAYAKGVPDRKVIIKHALKNAMIPIITIIALDIPYFFGGALIIETVFSWPGM
                     GRLMYQAVISSDYNLAISCLMFLAIITLISNLIADLLYVLVDP"
     gene            complement(954..2324)
                     /locus_tag="PLD15_06610"
     CDS             complement(954..2324)
                     /locus_tag="PLD15_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPB63612.1"
                     /translation="MKFTELIETVPDYKKFFTVEEMDRRSLALAEKYPDRVKVYEAGK
                     SRAGHPIYVLEIGKGSKNALLFGCPHPNEPIGAMMLDYLSERLASDDELLSKFDYTWH
                     MIKVIDVDGTKLNEGWFNDSSSIRKYAANFYRPPGHEQVEWTFPIDYKTLHFHSPLPE
                     TEVLMRLMEDKKPQFMYSLHNSGFGGVYYYISDDVPELYETFSKLPGLFGLPLNLGEP
                     EAPYLTELAPAIFKLFGMAQDYDYLEKNLGPDKDPASVIKAGTSSDDYASSVANTFSL
                     VCEMPYYYDPRVEDQSEADISRKEALRFNYEKSVESFNFTKEIMTSLEPEIKNRNSPF
                     YRVFKNVLETYPDQLQAQKNWIENDPSLERKAKKAEVFDNKIVSQFYQSLMLGMLRRL
                     IAENNDGSAKLSAVANMADEAFDRKIEYLEENLDYTTIPIKSLVCVQLLAGLNLADYL
                     QRKSDK"
     gene            complement(2387..4471)
                     /locus_tag="PLD15_06615"
     CDS             complement(2387..4471)
                     /locus_tag="PLD15_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB63613.1"
                     /translation="MKKLLISTLLLLFAVMTFAQISPEEAVPMIEGKGIITAADDSPL
                     TFEEFKTAVEKAFPGKGQLITGTGEVSRADFAMAMVKVLGLEAEANVVEEICTTAIDE
                     WEAPEEAWGALTVAYRSNRQILDFRYGHVIEATSPITKKEAAVSIYMAMNPPVKGGIA
                     TTAVAADAPGFNTIFTSSGLTWTICNIIGDGYTGTDNNGFYFPRMIKRMPSLENGLMK
                     ANEDGSLTITYELRKGMKWHDGTPVTARDAKFQWEVMTSGAPVTANYFENSVSEVTII
                     DDLTFSITLPEALSNAELGSSVYAYYFGWFQLPEHVYRKDFEQAKASGMWDSFVDKAT
                     KNPVMTGPYKLKEYAEGQFVILEAFDDYFMGRPNIDQIVMKIIPDSDVTFASTLNGEV
                     DFGRYTLDLKQSIQLQNQLPNVFSVFFTPNIAYDNLNLNLRDPKNTSNPNKFFGDKRV
                     RQAVLHAIDRQQISNVVYSGLAEVVDTWITDLHQMRDALKSPLVKHYEYNPARAKELL
                     AEAGWKLNNRGILEKDGVVFEFSIVGPAGSTQYQMMAQIIQSMLKQVGISVKVDLKPS
                     LVVWTEIMPYGDFDALLSGWGYGVSDDASNYWTTDLIPSDANYWGGTNYTGWANAEND
                     EIIYAAARELDPAKKVALYEKHFALWTEELPVLPLVVAPTPHFAKKYIKSFDSGYDNG
                     LGWIIQNWYIQK"
     gene            complement(4594..5445)
                     /locus_tag="PLD15_06620"
     CDS             complement(4594..5445)
                     /locus_tag="PLD15_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPB63614.1"
                     /translation="MWAFLDGEFVEEKDRHIKLENRGLTFADGLFEVIRTIENKILFF
                     EDHFSRMQKSAAHFDITLPYGSGYVKNAALELIKKNGIEDGELYLELTRGTDLHREHR
                     FPPKRTPSTFFMLALPLRHIDRESWKKGVKLLTYPDLRHRLCEHKTINLLPNVLAKNY
                     AYSNGGYEALMYREEGSYRYVTEGGSSNYFMVKDGVIHTPEIDNILPGITRGKVAKMI
                     CELGYELHERRVGVEEFLAADEVFLVSTVSRVMPVHSIDDFHFNAPGAVTLKVQEAYE
                     KIFFSFL"
     gene            5827..>7756
                     /locus_tag="PLD15_06625"
     CDS             5827..>7756
                     /locus_tag="PLD15_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63615.1"
                     /translation="MKKLFILLLFILPVLVLARNVLVFPNADVYYPGDYEDLAVYIGN
                     TFEFIRQKAIDLIGNDPGRINIVLLDKGTDTNGVALPFNHKTIHIYLWPSDAALSNRL
                     NVSNFYRQLLIHEFTHIVHLTYTTGIPAFISRILLGTELLSPQNLSPFIEGVTVFAES
                     TSYLSEGRLNNPLWGRELAYQNYVAGVFPDLAYALAVSREDYRSGALYYNYMAAFYDY
                     LVRRFGIDAVKDFHREKAGRLLPVIGTITSAKNAFGATLDDLYSDWEKEVALRSTAYA
                     TLTEIKTIENGQIMDMAGTGEGVVFSYIRNGEVSAWVGAYYRGLGEITDSGFDRRLSD
                     FGATSLKWKDGSTYLMTSVAERGRVSREIWQQRSSINVRLLDKGEMSAFDVFDGKLVT
                     AVYNKKEGVSTVYFDKMEVLRLPWLVRDMIVTEDSSYVMLLSRNGTNGAIGFFGEGEL
                     KIILDDPYMKGRGIDVRNGLVVYTAAYEAGYMDAFAVDLSTGEIYRLTEGANLEKAVL
                     SEDYIYGFGQSRLAKGMSIYRFDYSAVPYEVKEVESDSFEPAESEYESGDYLKKAFLH
                     FVNPILRAPLIEYDGKDFSLGFLFLNMSLDGEHTLTVNPSFNLGTLKPSLTVQYSGRI
                     IGGIDLALDLNLGKTDLPL"
BASE COUNT         1770 a         1928 c         2041 g         2017 t
ORIGIN      
        1 cgggtcgacc aacacgtaga gcagatcggc gatgaggtta gagatcaggg ttattatcgc
       61 cagaaacatc aggcagctta ttgccaggtt gtagtcgctg gatatgacgg cctggtacat
      121 gagacggccc atacccggcc aggagaaaac tgtttcgatt atcagcgctc ccccgaagaa
      181 gtaggggata tcgagcgcga ttatcgttat gataggtatc atagcgttct tcaaagcgtg
      241 ctttattatt accttcctgt cgggaacccc cttggcgtag gctgtcctga catagtcctg
      301 gtccagcact tcaagcaggg aggttcttat gtacctaacc caggaggcca cctgaaccag
      361 ccccagaatc gcagccggca ggatgaggta tctcagcctg tccacgaaaa tgttccaaaa
      421 acccgtgaca ccgtaggttg ccattcccga aactggcagc cacttcaggt tgacactgaa
      481 gatgattatt gcgatcaggg caaaccagaa actcggcaga gagatcccga tgaaggcgaa
      541 aaccgtcgta gtgtagtcga aggtcgagta ttttttcacg gccgacagta tgccaatagg
      601 cagcgccacg accagaccga tcagccaggc cgtgatcgta agtatcagag tgttgggaag
      661 cctggaggcg atcaggtcta tgaccggtgt cttgtacatg ctggaatatc caagatctcc
      721 tgtgagtacg ctcttcagcc aggtgaaata cttgagcggc agcgggtcgt ccagccggta
      781 gtactctctg agttctttca ttcgctgggg atcgttcgcc atcgccctgg ggttctgcat
      841 tcgcatctgg agaagtggat cgcccggcat cgagttcatt attacgaaga ttattatgga
      901 tatcgcaaag aagatcggta tgagttcgag gaaccgcttc agaaaatata ccttcactta
      961 tcgctcttcc tttgcagata atccgccagg ttcagccctg cgagcaactg aacgcagacg
     1021 agactcttta ttggaatagt cgtgtagtcc aggttctcct cgagatactc gatcttcctg
     1081 tcaaaggctt cgtcggccat gttcgctacc gcgctcaatt ttgccgagcc atcgttgttc
     1141 tcggctataa gccgtctaag cataccaagc atcagcgatt ggtagaactg gctcacgatt
     1201 ttgttgtcga agacctcggc ctttttagcc tttctctcga gggagggatc gttttcgatc
     1261 cagttcttct gagcctgcag ctgatcggga taggtctcca gaacgttctt gaagaccctg
     1321 tagaagggtg agtttctgtt ctttatctcc ggttctagcg aggtcatgat ctctttggtg
     1381 aagttgaaag attcaacgga cttttcgtag ttgaatctga gggcctcttt tctggagata
     1441 tctgcctctg actgatcctc gacgcgcgga tcgtagtaat atggcatttc acagacgagc
     1501 gagaaggtgt tggccacact cgaggcgtag tcgtccgagc tcgttccggc ctttatcacg
     1561 cttgccgggt ctttgtccgg accgaggttc ttttcgaggt aatcgtagtc ctgcgccatt
     1621 ccaaaaagct tgaatatggc gggagccagt tctgtcagat acggtgcctc aggttctccg
     1681 aggttaaggg ggaggccgaa gagaccgggc agcttgctga aggtctcgta aagctcgggg
     1741 acatcgtcgc ttatgtaata ataaacgcct ccgaagccgg aattgtggag ggagtacatg
     1801 aactgaggct tcttatcctc catcagtctc atcaacactt cggtctccgg gagtggagag
     1861 tggaagtgga gcgttttata gtcgatcggg aaggtccatt cgacctgctc gtggcccggt
     1921 ggccggtaaa agttggccgc gtattttctt atggaagagg agtcgttgaa ccagccttcg
     1981 ttcaacttgg tgccatcgac atctatcacc tttatcatat gccaggtgta gtcgaacttg
     2041 ctcagcagct cgtcgtcgct ggcgagtctc tcgctgaggt agtcgagcat catcgcgcct
     2101 atcggttcgt tcgggtgggg gcagccgaaa aggagcgcgt ttttggagcc tttgcctatc
     2161 tcaagaacgt atataggatg gcccgccctg gatttcccgg cctcgtaaac cttcactcta
     2221 tcggggtatt tctccgccag ggccagtgat cttctgtcca tctcttcgac ggtgaaaaat
     2281 ttcttgtaat cgggaactgt ttctataagc tcggtgaatt tcatgtcaaa cctccggaat
     2341 tatgatagag cggggacggg atcgacccgt ccccgccgaa agagaattac ttctgtatgt
     2401 accagttctg gattatccag cccagtccgt tatcgtaacc ggaatcgaag ctctttatgt
     2461 acttcttcgc aaagtgcgga gtcggcgcaa caaccagcgg gagcaccggc agctcttcgg
     2521 tccagagcgc gaagtgcttt tcgtagagag cgactttctt ggccggatcg agctctctgg
     2581 cagcagcata gattatttcg tcgttctcgg cgttggccca gccggtgtag ttcgtaccgc
     2641 cccagtagtt ggcgtcggac ggtataagat cggtggtcca gtagttggat gcatcgtcgc
     2701 tgactccgta tccccagccg gagaggagcg cgtcgaaatc tccgtaaggc attatttcgg
     2761 tccagacaac cagagaaggc ttgagatcga ccttaacgga tatgccgacc tgcttgagca
     2821 tgctctgaat tatctgtgcc atcatctggt actgcgtgct tcctgccgga cctactatgg
     2881 agaattcaaa aacaactccg tccttctcga gtatgcctct gttgttaagc ttccagccag
     2941 cctcggctag cagttccttc gccctggcgg ggttgtactc gtagtgcttt acgaggggac
     3001 tcttgagggc gtctctcatc tggtggaggt cggtgatcca tgtatccacg acttcggcca
     3061 gacccgagta aacgacgttg cttatctgct gtctgtctat cgcgtggaga acggcctgtc
     3121 ttactctctt gtcaccgaag aatttgttgg ggttcgaagt gttttttgga tctctgaggt
     3181 tgaggttcag gttgtcgtaa gcgatgtttg gagtgaagaa gacgctgaac acgttaggaa
     3241 gctgattctg aagctgtatg gactgcttca ggtctagcgt gtatcttccg aaatcgactt
     3301 cgccgttgag cgtggaagcg aaggtaacgt cgctgtcggg gattatcttc atcactatct
     3361 ggtctatgtt cggtctgccc atgaagtaat cgtcgaaggc ttctagaatg acgaactggc
     3421 cttcggcata ttccttgagc ttgtaaggac cggtcataac tggattcttg gtagccttat
     3481 caacgaaact gtcccacata ccgcttgcct tggcctgttc aaaatccttc ctgtatacgt
     3541 gttcggggag ctggaaccag ccgaagtaat aggcgtaaac cgaggaaccc agctcggcgt
     3601 tgctaagcgc ttctggaagg gttatagaga aggtgagatc gtcgattatc gtgacctcgg
     3661 aaacggaatt ttcgaagtag ttggcggtca ccggggcgcc gctggtcatt acttcccact
     3721 ggaatttggc atcccttgcc gttaccggtg tgccgtcgtg ccatttcata cctttgcgaa
     3781 gctcgtaggt aatggttaga gaaccgtctt cgtttgcctt cattagaccg ttctctaggc
     3841 ttggcattct ctttatcatt ctcgggaaat agaagccgtt gttgtccgtt cccgtgtatc
     3901 cgtctccgat tatgttgcag atcgtccatg tgagaccgga agaggtgaag atagtgttga
     3961 aaccgggagc gtcggcggct actgcggttg tcgcgattcc ccccttgacg ggaggattca
     4021 tcgccatgta gatggaaacg gcggcttctt tcttagtaat aggagaagtg gcctctatta
     4081 cgtgaccgta cctgaaatcg agaatctgtc tgttgcttct gtaggctacc gtcaatgcgc
     4141 cccatgcttc ttcgggagct tcccattcgt cgatagcggt ggtgcagatc tcttccacga
     4201 cgttcgcttc ggcttccagc ccaagaacct tgaccatggc cattgcgaag tcggctctcg
     4261 agacttctcc agttccagtt ataagctgcc ccttgccggg gaaggccttt tctacggcgg
     4321 tcttgaactc ttcgaaggtc agtggggaat catccgcggc ggttattatc ccctttccct
     4381 ctatcatggg aacagcctct tcgggagaga tctgggcaaa ggtcatgacc gcaaagagca
     4441 gcagtaacgt ggaaattagg agctttttca ttatgaaaca cctccaagaa agatgatgtg
     4501 gtactacaat tatagatact attatatatc agaacggctt cagatcggga agataaagcc
     4561 aaccgacaag tacattttac caaactcccc cgatcataaa aacgagaaga aaatcttctc
     4621 ataagcttct tgaactttca aagtaacggc gcccggtgca ttgaagtgaa aatcatctat
     4681 ggaatgcacg ggcatgacgc gtgagaccgt gctgaccagg aagacctcgt cggcggcgag
     4741 aaactcctct acccctaccc ttctttcgtg cagttcgtag ccgagctcgc aaatcatctt
     4801 cgcgaccttt cccctcgtta tccccggtag tatgttgtct atctccggcg tgtgaatgac
     4861 tccgtccttc accatgaaat agttggagct cccgccttcg gtaacatatc tgtagctacc
     4921 ctcttctcta tacatgaggg cttcgtaccc accgttcgaa taggcgtagt tcttggccag
     4981 gacgttcggc aaaaggttta tcgtcttgtg ctcgcagagt ctgtgtctca ggtccgggta
     5041 tgtcagcagc ttaacgccct tcttccagct ctctctgtct atgtgcctca ggggtagtgc
     5101 cagcatgaag aaagtggaag gtgttctctt agggggaaat ctgtgttctc tgtgaagatc
     5161 ggtgccgcgt gtgagttcca ggtacagctc tccgtcctca atcccgttct tctttataag
     5221 ttcgagagcg gcgtttttga cgtagccgct gccgtaaggg agagtgatat cgaagtgagc
     5281 ggcgctcttt tgcatcctcg aaaaatgatc ttcaaagaag aggatcttgt tttcgatggt
     5341 ccttatcact tcgaagagac cgtcggcgaa ggtcaaaccc ctgttctcca gcttgatatg
     5401 cctatctttt tcttcgacga actccccgtc cagaaatgcc cacataatat taacctccga
     5461 aagtgaaatc agtttctcct taaagtctat tatgcaatag gaaaatgtct tttggaacgg
     5521 aagggttgaa aacaatgtta gaatatactc gaaacgggca ggccagatca cattaacccc
     5581 tcgtctttag gttattttcc ggtgaacaac gctggtacgg ggtcctccgg aaattcttcg
     5641 aggtttctga aaaatcaatc cccggtgccc gggttctgcc cgagggtatt ctatcgcagc
     5701 ggctgaccga ccggatgatc gatctgagag gagtcgtaag ggaactggaa aaagcccggg
     5761 acgatttcaa aggatcgaac atccacagga cgaccgaaat cttttcggca ggagggtgag
     5821 ggtatcatga aaaagctttt tattttactt ctgttcatat tgcccgttct tgttttggcc
     5881 agaaatgtgc tcgtctttcc aaacgccgac gtttactacc ccggggacta cgaagatctg
     5941 gctgtctata taggcaacac tttcgaattt ataaggcaga aggccataga tctcatcggg
     6001 aacgatcccg ggagaatcaa catcgtactc cttgacaaag gcaccgatac gaacggcgtt
     6061 gcgcttcctt tcaaccacaa gacgatccat atatatctct ggccatcgga tgccgcattg
     6121 tcaaacaggc tgaacgtttc caacttttac aggcagttgc tgatccacga attcacgcac
     6181 atcgtccatc tgacctatac tacgggcata ccggccttta tctctagaat ccttctggga
     6241 acggagctat tgtcgcctca gaatctctcg cccttcattg agggtgtgac cgtcttcgca
     6301 gaatcgacca gttatctttc agagggaaga ttgaacaacc ctctctgggg aagagaactg
     6361 gcctaccaga actatgtggc cggcgtattt cctgatctgg catatgctct cgccgtttca
     6421 agggaggact accggagcgg cgccctttac tacaactata tggcggcctt ttacgattat
     6481 ctcgtccgac gcttcggaat agacgccgtc aaagattttc atcgtgagaa agcgggcagg
     6541 ctgttgcctg tgatcggtac gatcacctcg gctaaaaacg ccttcggcgc cactcttgat
     6601 gatctataca gtgactggga gaaagaagtg gctttgagat ccactgccta cgcgactctg
     6661 accgaaatca aaaccatcga gaacgggcag atcatggaca tggcaggcac gggagagggc
     6721 gtggtcttct cttacatacg caacggagag gtcagcgcat gggttggagc ttactatcgc
     6781 ggtcttggag agataaccga cagtggtttc gatagaagac tctccgattt cggggctacc
     6841 tcgctcaaat ggaaagacgg aagcacatat ctcatgactt ccgtcgctga gagaggccgt
     6901 gtatcgcggg agatatggca gcagcgttcc tcgatcaacg tcagactcct cgataaaggc
     6961 gagatgtcgg ccttcgatgt cttcgacggg aagctggtaa cggccgttta caacaaaaaa
     7021 gaaggcgtat cgacagtcta cttcgataaa atggaagttc tgagactccc ctggctggtc
     7081 agggatatga tcgtcacgga agatagcagt tacgtcatgc tcctctccag aaacggtacc
     7141 aacggagcga taggcttttt cggcgaggga gagctcaaga taatcctcga cgatccttac
     7201 atgaagggcc gggggatcga tgtccgtaat ggactggtag tttatacggc cgcttacgag
     7261 gcgggttata tggacgcctt cgcagtggat ctttccaccg gggaaatcta cagactcacc
     7321 gagggggcca acctcgagaa ggccgttcta tctgaggact atatatacgg tttcggacag
     7381 tcacgcctgg cgaagggtat gtccatttac cggttcgatt actcggccgt tccttatgaa
     7441 gttaaagagg tggagagcga cagcttcgag ccggccgaat cggagtacga aagtggagat
     7501 taccttaaaa aggccttcct tcactttgtg aaccctatcc tgcgggcacc cctaatcgag
     7561 tacgacggta aggacttttc actgggcttt ttgttcttga atatgtcgct cgacggagag
     7621 cacacgctca ccgtgaatcc ttcgttcaat ttaggaacgt taaagccttc cctgactgtt
     7681 caatacagcg gaagaataat aggcggtata gatctcgctc tcgatctcaa tctcgggaag
     7741 accgaccttc ctttgg
//
LOCUS       DAOOLY010000106         7602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_11403_length_7602_cov_5.036306, whole genome shotgun sequence.
ACCESSION   DAOOLY010000106 DAOOLY010000000
VERSION     DAOOLY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11403_length_7602_cov_5.036306"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PLD15_06630"
     CDS             <1..1060
                     /locus_tag="PLD15_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63616.1"
                     /translation="AAQTTLGGYTGLVFGGLYLLAVWKRGLFKYILIGDILVSLLLSL
                     FNGFLRGFNAATIISLMVFLILRREREGAAVVKSGDRALKLLTGIVLAGVLIASVLPL
                     LVLVWISLSGLSTTYIDSFLPRYFTLDNFVRVVGEEGIISTLLNTLFVSLVTGLAVPL
                     LTFQLALALRKIKNSLSDIAIVILNTVGVIGGMHTLVPLYSIFRTMGMIDSYFPVVLV
                     YISHAIPFSVFTIRTYLESVPESLEDQAKIDGVGPVAFGFRILLPLSRPVLTTSFIVA
                     FLGAWNGFLAPLLFLLSDSKYTMGVKLYTYVGSLGSGNPQWNLFGAAATINLLVMGVI
                     FYTFRGYLGKTPLSENYE"
     regulatory      1051..1178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1170..2564
                     /locus_tag="PLD15_06635"
     CDS             1170..2564
                     /locus_tag="PLD15_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB63617.1"
                     /translation="MKLRELTVLLWLLLNLPCLSFEVLLNDESIRSWDGSLLQYCYDL
                     PQGKAIPLLEILPPVVERDGCFLETERGRLSDVPMDSLLVWEEGDWFLHLEGEGVKIL
                     SVAVYGEPIDNDYLQVWLDWEGITALKESIQRFSSINGLRIETREVPKASSKLVSVVR
                     ARGEVPDVIMVQSSDISRLVYEEAIQSLDYIPVQGLSGLSSFNYEGRSWAIPFYFDCQ
                     LVFYNRGLVGEIDPGWTLEDLENLSKDPGSGGAGWNIYSAYWFIPFQLGFGKRDLIED
                     GRVTVLDGPTQEALGYLVERVDDGTFRALERDPMVTLFASGKVGIILSGSYMIEQFER
                     IGMDFGLAPYPEPLRPVLDYKGLAITRKTRNPILARRLIEFLTSKYVQASFCKASYKI
                     PSRREALDGMELNFAISSSLERGYPLPADPLYELYKDTMWKLIRLAISDRLGVREVLE
                     KGQKIIDENSGGVQ"
     gene            2561..3967
                     /locus_tag="PLD15_06640"
     CDS             2561..3967
                     /locus_tag="PLD15_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63618.1"
                     /translation="MKKLLAIMFTVLIAIASIAGITVTCNDSTFVFDRGDFPSLRVVT
                     RFEGPQPSSDRNGSDYIEYEAIPLISVLEYVGGMREGDLLKIEADDGYSKLFPFGAIY
                     GRTALGNPYIGYREEKPYFIFVPDDGEVSNEDMLASLGEEYSHFYDGLPSSKGLMVKS
                     VSTIRIETSRTVSPLIMETAAFPGPSPDWTVEIKGSGKKEISSIDFEADEADGGHYVE
                     ISLERKGETNTYRAIPLWLVVAAAREDEQKRFSQYRELWEGGFDITLTSVDGYSVTFN
                     TLDVPIEAIFIADEENGEKIAPAIVGEISGKLWVKGLASIELDTERYSYEDPRLSVSL
                     VLKAGGLEREFTIADLEAKSYYVENYGQYITSSGGKHGGLYGGVELVKLVGELVELKP
                     TSTIRTVASDGYETTYSAGEIESADGIWIVAFKLDGDYLPEDPGYFRTIKVGPNMPAI
                     ESRRSAKMVEIIEVIDLE"
     gene            4059..6434
                     /gene="leuS"
                     /locus_tag="PLD15_06645"
     CDS             4059..6434
                     /gene="leuS"
                     /locus_tag="PLD15_06645"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPB63619.1"
                     /translation="MYDFRVLEKKWQDYYAREKPFNIDLDKAERPFYNLMMFPYPSAA
                     GLHIGNMFSFIGSDVYGRFMKLKGYDVFEPIGFDAFGIHSENYALKVGKHPAELTPKS
                     IEYFREEQLKKLGNIFDWDSEAITSSPEYYKWTQWIFIQLYKRGLAEKRDSSVNWCPS
                     CKTVLADEQVIDGHCERCSSEVDKREMSQWFFKITEYAEKLLSDLDWLDWTDTTKNLQ
                     RAWIGKSAGASITFDVSGMEEKIEVFTTRPDTIYGVTYIVVAPEYPLVDKLITPVMEE
                     PFEEFRNNVKNMDMATRTSISRPKNGIFTGSYALHPLTGEELPIWIGDYVLAEYGTGA
                     VMAVPAHDERDFAFARKYGLEIRQVIECDPSELPYTEKGVMMNSDRYTGMKSTDFIDR
                     IDTIDASIKGSVNYHLHDWCISRQRYWGPPIPMIYCDRCGTVPVPDEDLPVMLPKTDD
                     YIPDGSGKSPLAKSPEFVNTTCPVCGGPARRETDVSDNFLDSAWYYLRYLSPKNEEIA
                     FERELVEKWCPVNMYIGGNEHATLHLMYSRFIAMALHDMGYLSFEEPFASFRGHGLII
                     KDGEKMSKSKGNIVNPNQYFDSHGVDALRTYLMFMGTFLEGGDFRDSGMDAMKRFLNR
                     VWDIANMGEGKSSLNLKIKMAETIEKVEYSITSIKPNTAIAAIMEFVNEASKESAIER
                     QLVIDMAKLLSPFAPFLCEEIYSIKGGKKRTILEDGFPSGYEKYASMANVELPVQISG
                     KMRGKVTLEQNSSQEKAMEAIMKDEKLASMLEGKTVRKIIYVQDKIINIIV"
     gene            7133..>7602
                     /locus_tag="PLD15_06650"
     CDS             7133..>7602
                     /locus_tag="PLD15_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPB63620.1"
                     /translation="MKKTMILILFALVSLVAFSQKNIVDIAVESGNFKTLVAAIETAG
                     LADVLKGSGPFTVFAPTDEAFENLPAGTVKALLDNVSKLRSVLLYHVVSGEYSFESIV
                     LYPALQTLHGQSLAIDTRGGLKIDGAAVTVKDIKASNGVIHIIDTVMIPREEANL"
BASE COUNT         1982 a         1915 c         2016 g         1689 t
ORIGIN      
        1 agcggcgcag accacgctcg gcggctacac tggcctcgtc ttcggaggtc tgtatctact
       61 cgctgtatgg aagaggggat tgttcaaata tatcctcatc ggcgatatcc tcgtcagtct
      121 tctgctcagt ctgttcaacg gatttctccg cggtttcaac gccgccacga taatctccct
      181 gatggtcttt ctgatactca gaagggagag ggagggtgca gccgttgtaa aaagcggcga
      241 tagagctctg aaacttctca ccgggatcgt gctggcgggc gtactgatcg cctctgtcct
      301 cccccttctc gttctcgtct ggataagctt atcaggactc tcgacaacgt acatagattc
      361 atttctcccc agatatttca ctctggataa tttcgtgagg gtggtaggag aggaggggat
      421 aatctcaaca cttttaaaca cacttttcgt ttctctcgtt actggactgg ccgtgccttt
      481 gctcacattt cagttagctc tcgctttgag gaagatcaaa aactcgctct cggacatcgc
      541 cattgtcata ttgaacaccg tgggcgtgat agggggtatg cacactctgg ttccgctgta
      601 ttccatattc agaacgatgg gaatgataga cagttatttt ccggtggtcc tggtttacat
      661 ctctcacgcc ataccctttt cggttttcac cataaggact tacctcgagt ccgtgccaga
      721 gagcctggag gatcaggcga agatcgacgg ggtcggaccg gtcgccttcg gtttcaggat
      781 acttctgccg ctctccagac cggtgctgac aacctcattc atagtggcct ttctgggcgc
      841 ctggaacggt ttcctggcgc cgcttctatt ccttctgagc gacagcaaat acacgatggg
      901 agtcaagctc tacacctacg tcggcagtct cggttcgggc aatccgcagt ggaacttatt
      961 cggtgcggcc gccacaataa acttgcttgt catgggcgtc atattctata cttttagagg
     1021 gtatctgggc aagactccgt tatcggagaa ttatgaataa tcctccgagc atttcctcct
     1081 gaaaggagtt cgctgtctcc catggagaaa tgccgatagg gtatgactgg aaacggtcat
     1141 gcctcccgtg cttggaaagg aggtatattt tgaagcttcg cgagctgaca gtcctgttgt
     1201 ggctgctgct gaatctgcca tgcctgtctt tcgaagtgct gctgaacgac gagtcgatca
     1261 ggagctggga cgggtcacta ctccagtact gctacgacct tccccaagga aaggcgatac
     1321 cgcttctgga gattctcccg ccggtcgtcg agagagatgg gtgttttctg gaaaccgaaa
     1381 ggggccgatt gtccgacgta ccgatggact cgcttctcgt gtgggaggag ggcgactggt
     1441 ttctccatct ggagggagaa ggcgtaaaga tactttccgt ggccgtttat ggcgaaccca
     1501 tcgataacga ttacctccaa gtgtggcttg actgggaggg tataaccgcc ctcaaagagt
     1561 cgatccagag attctcctct atcaacggcc tcagaatcga aacccgggaa gtcccgaagg
     1621 cttcgagcaa actcgtctcc gttgtcaggg cacgcggcga agttccagac gtgatcatgg
     1681 ttcagtcgag cgacatctcg cgactggtat atgaggaggc cattcaatct ctcgattaca
     1741 ttcccgtgca gggtctgtcg gggctgtctt cgttcaacta tgaagggaga tcgtgggcca
     1801 tccccttcta cttcgattgc cagctggtgt tctacaaccg tggactcgta ggggaaatcg
     1861 atcccggatg gacgctcgaa gatctcgaga acctttcaaa agatcccggg agcggcgggg
     1921 ccggctggaa catttactcg gcctactggt ttatcccttt ccagcttgga ttcggcaaga
     1981 gagatctgat cgaggatgga agagttacgg tcctggatgg gcccactcaa gaggccctcg
     2041 gctatctcgt cgagagggtc gacgacggaa ccttcagggc tctcgagcgc gacccgatgg
     2101 tcactctctt cgcttcggga aaggtcggga taatactctc gggctcctac atgatagaac
     2161 agttcgagag aatagggatg gatttcggac tggctcccta tcccgaacct ttgaggcccg
     2221 ttctggatta caaaggcctg gccataacaa gaaagactcg caaccccatc ctggccagga
     2281 gattgataga gttcctcaca tcgaagtacg tgcaggcgag cttctgcaaa gcttcctaca
     2341 agatcccctc gagaagggaa gcgctggacg ggatggaact gaactttgct atatccagca
     2401 gcctcgagag aggctacccg ctaccggccg atccgctgta cgaactttac aaagacacca
     2461 tgtggaaact gataaggctg gccatatccg acagactggg agtgagagaa gtgctcgaga
     2521 agggccagaa aatcatagac gaaaattcag ggggtgttca atgaaaaaac tactcgctat
     2581 catgttcacg gtgttaattg cgatcgcttc gatagccggg attaccgtta cctgcaacga
     2641 ttcgactttt gtcttcgaca ggggcgattt tccctcgctg agagtcgtaa cccgtttcga
     2701 gggaccgcaa ccgtcttcgg atcgaaatgg cagcgattac atcgaatacg aggcgattcc
     2761 tctgatctcc gtcctcgaat atgtcggtgg tatgagagaa ggagacctgc tgaaaataga
     2821 ggccgacgac ggatattcga aactcttccc tttcggcgcc atctacggta gaacggccct
     2881 gggcaatcct tacatcggat acagggagga aaagccttac ttcatcttcg tgcccgacga
     2941 cggcgaggta tcgaacgaag acatgctcgc ttcgctcggg gaggagtact cacacttcta
     3001 cgatggcctg ccctcatcca agggtttgat ggtgaagagt gtatcgacga ttcgtataga
     3061 aacttcgaga acagtctccc cgttgataat ggaaactgca gcctttccgg gcccttcgcc
     3121 cgactggacg gtcgaaatca agggaagcgg aaagaaggaa atctcctcaa tcgatttcga
     3181 ggcagacgaa gcggacgggg gacattacgt ggagatatct ctcgaaagaa agggagaaac
     3241 gaatacatac agggccattc ccctgtggct agtcgtggcg gccgccagag aggatgagca
     3301 gaagcggttc tcacagtaca gagaactctg ggagggcggc ttcgatataa cacttacctc
     3361 cgtcgatggc tattcggtta ccttcaatac tcttgacgta ccgatcgagg ctattttcat
     3421 agccgatgaa gagaacggcg aaaagatcgc gccggccatt gtgggcgaga tctccggaaa
     3481 actgtgggtg aaaggactgg catctatcga actggataca gaacggtact cttacgaaga
     3541 tccccgtctc tcggtttcac tggtgcttaa agccggtgga ctggaaagag agttcacgat
     3601 agcagatcta gaagcgaaga gctattacgt cgaaaactac ggtcagtaca tcacctcgtc
     3661 ggggggaaag cacggtgggc tgtacggcgg tgtggagctg gtaaaactcg tcggagaact
     3721 ggtcgagttg aaaccgactt cgacgatcag gacggttgcg agcgacggtt atgaaacgac
     3781 ttactctgcg ggcgaaatcg aaagcgccga cgggatctgg atagtagcct tcaaactcga
     3841 cggagattac cttcctgagg acccgggata tttcaggacg atcaaagtcg gtccgaacat
     3901 gccggcaatc gaaagccgtc gatctgcaaa aatggtagag atcatcgaag tgatcgacct
     3961 ggaatgaagc tcatctggaa atttactttc acatacgcat gttataatcc atctaagttg
     4021 cttttctcag gctttatact cattcgggga ggtagcatat gtacgatttc agggtgctcg
     4081 agaagaagtg gcaggattac tacgccagag agaaaccatt taatatagac ctcgataagg
     4141 ccgaaaggcc tttctacaat ctcatgatgt ttccctatcc ctccgccgcc gggctgcata
     4201 taggaaacat gttctctttc atcggatcgg acgtatatgg aaggttcatg aagctcaagg
     4261 gttacgacgt cttcgaaccg atcggtttcg atgccttcgg catacacagc gaaaactacg
     4321 ccctgaaggt gggcaaacac ccggccgagc tgacaccgaa aagcatagag tatttccgcg
     4381 aggaacaact gaagaaactg ggaaacatat tcgactggga cagcgaggcg ataacctcct
     4441 cgcccgagta ctacaagtgg acccagtgga tcttcataca gctgtacaag cgaggactgg
     4501 ccgagaagcg cgattccagc gtcaactggt gcccctcctg caagacggtc ctggccgacg
     4561 aacaggttat agacggccat tgcgagcggt gcagcagtga ggtggataag cgcgagatga
     4621 gccagtggtt cttcaagatc accgagtacg ccgagaaatt gctctccgac ctcgactggc
     4681 tagactggac cgatacgacc aagaacctcc agagggcctg gatcgggaaa tcggccggcg
     4741 cttcgataac tttcgacgtt tcaggaatgg aggagaagat cgaagtcttc accaccaggc
     4801 ccgatacgat ctacggtgtc acctatatag tcgtcgcgcc ggagtacccg ctggtggata
     4861 aactaataac tcccgttatg gaagagccct tcgaggagtt cagaaacaac gtcaaaaaca
     4921 tggacatggc gaccagaacc tctatatccc ggccgaagaa cggaatcttc accggctctt
     4981 acgcgctcca tccgctcacg ggcgaggagc tgcccatatg gatcggcgat tacgttctgg
     5041 ccgaatacgg caccggtgcc gtcatggccg ttccggctca cgacgagaga gacttcgcct
     5101 tcgccaggaa gtatggcctc gagataagac aggtaataga gtgcgaccct tcggagctgc
     5161 cctacaccga aaagggagtc atgatgaaca gcgacaggta cacaggcatg aaaagcaccg
     5221 acttcataga ccggatagat accatcgacg cctcgatcaa aggctcggtc aactaccacc
     5281 tccacgactg gtgcatatcc aggcagcgct actggggccc gccgatccct atgatttact
     5341 gcgacaggtg tgggacggtt cccgttccag acgaagacct gcctgtgatg cttcccaaaa
     5401 ccgacgatta catacccgac ggcagcggaa aatcgccgct ggccaaaagc cccgagtttg
     5461 tcaacaccac ctgccccgtc tgcggaggcc cggccaggcg cgagaccgat gtctccgaca
     5521 acttcctcga ttcggcctgg tactatctga gatacctctc acctaaaaac gaggaaatag
     5581 ccttcgaaag agaactcgtc gaaaaatggt gtcccgtgaa catgtacatc ggtgggaacg
     5641 agcacgcgac actccacctg atgtattcga gattcatcgc gatggccctt cacgatatgg
     5701 gatatctttc attcgaagag cctttcgcat cgttcagggg ccacgggctt atcataaaag
     5761 acggagagaa gatgtcgaaa tctaagggca acatcgtcaa ccccaaccag tacttcgaca
     5821 gccacggggt cgatgccctc agaacctatc tgatgttcat gggcaccttc ctcgagggcg
     5881 gagacttcag ggacagcgga atggacgcga tgaagagatt cctcaaccgc gtctgggaca
     5941 tcgctaacat gggcgaaggt aagagctcgt tgaacctgaa gataaagatg gccgagacca
     6001 ttgaaaaggt cgaatactcg ataaccagca taaagcctaa cacggcgatc gccgccataa
     6061 tggagttcgt caacgaggcc tcaaaagaga gcgcgatcga gaggcagctc gtgattgata
     6121 tggcgaagct cctctcgccc ttcgcaccct tcctctgtga agagatctac agtataaagg
     6181 gcggaaagaa gagaaccatc ctggaagatg gcttcccctc gggttacgag aaatacgctt
     6241 cgatggcgaa tgttgaactg cccgtccaga tatccgggaa gatgagggga aaggtgacgc
     6301 tcgagcagaa ctccagtcag gaaaaggcca tggaagccat catgaaggat gaaaaactcg
     6361 cttccatgct cgaaggaaag actgtcagaa agataatata cgtacaggac aaaataataa
     6421 acataatcgt ctgaatatca cgattatgaa gaaagaccgg ccgaatggcc ggtctttttg
     6481 ttacggacgt aactcgtcac tttcagttat gaaccggtta cgaaaggcag aaaagcaaag
     6541 ccacaaacaa agccgatgcc tgatacttcg atagacagct aactactctc tcttattcat
     6601 ttcaataaat tcttcaagga aaatttctag actaattgaa atccataaac ctctctagac
     6661 gaagcttatg gaataaataa gaaatttaag aaagttataa tcggaaagct aagccagcaa
     6721 gcgggatatc cagagaacaa atgcgccttc ttcgtctcta ttcgtcctta aacgcttgca
     6781 gtcagttggg attgccctga aagatgatat tgcgagattt gcatgttcga ttataatgct
     6841 agaattctca tggagcggac atgagccgcg acaaaaagag gtggctagga tgccgggcaa
     6901 gaaggatgta aagaaagaag taaagaaaga gccaaaaaaa gaaaccccaa agaagtaata
     6961 gatacagtca cactcaaaaa agaggcgggc cagcgaaggc tcgcctcttt tatttaacct
     7021 tgctctctac agaagagcca gatgaaccgg caccagactt ttctcttctc aaataaaccg
     7081 gtgcaatcgt tgtagaatat cactgaacag aaaccaaaag gaggtgtgca agatgaagaa
     7141 aacaatgatc ttgattctct ttgccctcgt aagcctggtg gctttttccc agaaaaatat
     7201 agtagatata gccgtcgagt ccggtaattt caagacgctg gtcgcggcca tcgagacggc
     7261 aggcctggca gacgttttaa aggggagcgg acccttcacg gtcttcgccc cgaccgacga
     7321 agccttcgaa aaccttcctg cgggtacggt caaagccctt ctggataacg tctcgaaatt
     7381 gagatccgtt cttctctacc acgttgtatc gggcgaatac tcttttgaga gcatagtgct
     7441 ttatccggcc cttcaaacac tgcacggaca gtctctggcg atagatacgc gcggcggctt
     7501 gaaaatcgac ggagccgcgg ttacggttaa ggacataaaa gcctccaacg gcgtgataca
     7561 tattatcgat accgttatga tccccagaga agaggccaac ct
//
LOCUS       DAOOLY010000107         7523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_11559_length_7523_cov_4.006829, whole genome shotgun sequence.
ACCESSION   DAOOLY010000107 DAOOLY010000000
VERSION     DAOOLY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11559_length_7523_cov_4.006829"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..2077
                     /locus_tag="PLD15_06655"
     CDS             263..2077
                     /locus_tag="PLD15_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB63621.1"
                     /translation="MANSKRVFRSVLSDFKASALSLATFSILSKFVVASLSIPAVALF
                     LRSLVSMPVRSKSGSWEFFEYSGSFPGVVLVMIVVFFWLVGFLVEQSGLLLIASVKVR
                     GGVPKIREVLLKFLRTAPRIVGLSSFQAGVYTLCFVPLLAVFAFVTRNIIDSGNGGYP
                     SFFTGFFGWLLAAASLLVLVRLMLSWLFAMHFGLLCREKFLKALGSSKKLVKGNRMRL
                     AHILGRFWLLTVVILLVFSLAFRGGRELMVSYSREPTSFNIFRLSLFASAETVFITVF
                     WIVVAGLFSILVTRLFLELVAENNLEALEEAGTYLSKEPKIQKRPWILLLLGLFAFQA
                     TYFDYYGKLLVESEVDLPLVTAHRGSSLKAPENTMSAIIQAVHDGADIIEIDVQLTSD
                     GHVVLNHDRTLSKVAGVSKSVPEMTLEEIKAIDVGRRFSRNFAGERIATLEEVIEYMN
                     EVDTKIKLNIELKDYEKTPMIVQAVLDTLEKHEFTERIVITSTSRDRLAAVRESDPAI
                     RIGLIVTYITRDLWSLDVDFYSVSSTILNGAFMIRARSYGRDVHVWTVNDTESMLRYT
                     SMGVSSIITDRPETLSQLLIRKAQLSIFQRRVLAVLSF"
     gene            2177..2803
                     /locus_tag="PLD15_06660"
     CDS             2177..2803
                     /locus_tag="PLD15_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HPB63622.1"
                     /translation="MGVYAVVTADVVRSREHDLSLEILKETLEGFASGLLVKPFSVSR
                     GDELQAVLSDLEALPMLLRRLRYCVLPLRLRIGVGIGFIEDSAFLKAGSSWDMSGKVF
                     FDARSALDGAKKSKDAQTVVACEEENLRLFLNTVFILKDAIESDWTEKQWEAVHTYER
                     EGTYERAATALGVTAPNVQNHCAKAKWNAIKSVETNMSAALRERFSGI"
     gene            2889..3662
                     /locus_tag="PLD15_06665"
     CDS             2889..3662
                     /locus_tag="PLD15_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HPB63623.1"
                     /translation="MPLLLSVLAHLLADFIFQTDKTVEDRLALRFSGFFKHGITVFLT
                     MLALLLPYGFISSFLYSLAVSLIHIFLDIMKSSLERRRSAVASFYLFLIDQFLHIATI
                     ILLVPLFHLKVTASFEGLIGSMAAYTGIDPGRLPVGRMLFTAIVYLSVVFGGAVFIRK
                     LFDLIYRNEPDYLSRIMGSNAVLNNVRTGKVIGIFERLVVLTIYLTGNVASIGIVIAA
                     KSFARFKHFDNKDFAEYYLIGTLASVMLAIIGGIILRTF"
     gene            complement(3705..5048)
                     /locus_tag="PLD15_06670"
     CDS             complement(3705..5048)
                     /locus_tag="PLD15_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPB63624.1"
                     /translation="MKKALKFWDLVALEIGMTIGAGIFVYMPLAYRSAGAGTIMAFVF
                     AFLPMAVIMINVMLLGSTLPTTGGTFKYGAFLFSPKVAFLGLWAYLFGAFVGLFPLNA
                     LALASYMKGIWNDLSLVPVALIILTFFYVVNLLGLKMASRVEIISVALLFLAIAVYTV
                     PGMGSIDSKNLEAVFSTGMGSMIYASALLTFTFAGSNAVIELGGEVENARKNLPLSVI
                     FSLAVVLICYLLMAVVSFGVGGDTLENGTLNDVASNYLSGFLFYVFAFGGPILAIATT
                     INATYMWGTRSLLALCRLRVFPSKLGAVNRRGTPWVLLTIVWLLSSIMLISVGESGLN
                     LFAAFASIGGIAVIVPAMFAVFRLKRDPRLKEKAPAIVNKKWFGIIPVLGTAFSIMIM
                     LILLYQVGVDFSTSFFLLFIVWEVFGMIYFLFRLKYLDRVKKNPFSRNDLSAFDD"
     gene            complement(5195..>7523)
                     /locus_tag="PLD15_06675"
     CDS             complement(5195..>7523)
                     /locus_tag="PLD15_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPB63625.1"
                     /translation="GLEDFLENAFEEGVDPADLREVFLKGFYSRLLSLFYGEDTILNS
                     FSGSEQNILVEEFRRLDIKQQHYARKRLASLLAQKIPVSDFARSGSAETSILKREVAK
                     KRRNKSIRRLFSEIPNLLQVLKPCMMMSPLSVSVFIDPERFRFDLVIFDEASQVFPED
                     SVGAIMRADQVVIVGDNKQLPPTSFFKISEPDVAELEEEESDLESLESILDECSTAGL
                     MEKKLLWHYRSRHESLIAFSNHHFYENRLNTFPSSNFDGPGCGISMTFVDNGIYDRAR
                     SRKNQEEALRVATMVMNHARSKPLVSLGVVAFSEAQQMAIMDCLELLRREDPSLEEFF
                     KEESPEPFFVKNLENVQGDERDEMIFSIGYGRDHLGRLTMNFGPLNKNGGERRLNVAI
                     TRARQKVTVVSSIKAGDIPADVQTPGVLALREYLNFAESGGHTGFLQSERRNREADFD
                     SPFEEEVYRELTECSLAVHSQVGCSSYRIDLAVVDDRRPGKYLLGIECDGAMYHSART
                     ARDRDRLRQKVLENLGWKITRVWSRDWVFDRKAERDRLLSEIEKARSNSSVEGESNTV
                     ETIPEENLQKLENDKPSLPVYERFNPPDLIELSRSGKLSSAGQLIQAVMEKEAPVHLD
                     EVVSRILGLYENHPTLLPAMNRNMYTQSGSISRKRLKQYLLFNLPKEKYELEGDFILN
                     RSETVLPRRATGANARKADHIPPVEVREAIRLCLKDAFSMNREDLKTDVSKLFGFRRV
                     GTTMNELIEKEIKFLIQNGQLESDTDRLSLSDGAK"
BASE COUNT         1761 a         1876 c         1892 g         1994 t
ORIGIN      
        1 ggacccacca tcgcgactta cgccggcatc gggggattga ttctcgctct ttgaggatta
       61 ccttacgtcc tctcttacta tctgtatcat ctcttcggaa atgaccggaa tctcctgacc
      121 atctgaacgc tcatctcaca gatacaaata cccgtcgttt tataacgatc gcgctttttt
      181 taccaaaccg tagcgagcct taagaaactc gcccgatcca ctgtggtgtc taaatggaaa
      241 gcatttccgg gagttgtact cgttggcaaa ttctaagaga gttttcagat cggttctttc
      301 ggatttcaaa gcctcggctt tgagtctggc tacattctct atcctgtcga aattcgtagt
      361 cgcttcgctc tcgattccgg cggtggctct ttttctcaga tctctcgtat cgatgccggt
      421 gaggtcgaaa tcaggaagct gggagttttt cgagtactcg gggagctttc cgggcgtggt
      481 tctggtgatg atcgtcgtct tcttctggtt ggtaggtttt ctggtcgagc agagcggact
      541 gcttctcatc gcttcggtaa aagtccgggg aggtgttccc aaaatcagag aggtgttgtt
      601 gaagtttctc agaaccgcgc caaggatagt gggcctttcc tccttccagg ccggcgttta
      661 caccctctgt ttcgttcctc tccttgcagt cttcgctttt gtaacaagaa atatcatcga
      721 ctccggcaac ggtggatatc cctctttctt cacaggcttt ttcggttggt tgctcgcggc
      781 agcctctctg ctggttcttg taaggttgat gctcagctgg cttttcgcta tgcacttcgg
      841 tctgctctgc cgggagaaat ttttgaaggc ccttggatct agcaagaaac ttgtaaaagg
      901 aaacagaatg agactggcgc acattctggg gcgtttctgg ctcttaactg tggtgatcct
      961 tctggtattt tctctcgcct tcaggggggg ccgcgaactt atggtcagtt acagcagaga
     1021 accgacatct ttcaatatat tcagactctc gctcttcgct tccgctgaaa cggtttttat
     1081 cacagtcttc tggatagtcg tcgcgggact gttttcaata ctggttaccc gcctcttcct
     1141 tgaactggtt gccgaaaaca accttgaagc tcttgaggag gcaggaactt atttgtctaa
     1201 agagccgaag atacagaaaa gaccctggat tctattgttg ttaggtcttt tcgcgttcca
     1261 ggcgacttat ttcgattatt atggaaaact cctggtggaa tcggaggtcg atcttccact
     1321 ggtgaccgcc cacaggggga gttctttgaa agcccctgag aataccatga gcgctataat
     1381 tcaggccgta cacgacggtg ccgatatcat agaaatcgat gtccagctga caagtgacgg
     1441 ccacgtagtg ttgaaccacg acagaacgct ctccaaagtt gccggagtaa gcaagagcgt
     1501 ccccgagatg actctggagg agataaaggc gatagacgtc gggaggagat tttcaagaaa
     1561 cttcgcgggc gagagaatcg cgacactcga agaagttatc gagtatatga acgaggtcga
     1621 tacgaaaata aaactcaaca tagagctgaa ggactacgaa aaaacgccca tgatcgttca
     1681 ggctgtcctc gatacccttg agaagcatga attcacggag agaatcgtga taacttctac
     1741 aagcagggac cgattggcag ccgtgagaga gagcgaccct gccattcgga ttggactgat
     1801 agtcacttac ataaccagag atctctggtc gctggatgtg gatttctaca gcgtgtcttc
     1861 taccatactg aacggagcct tcatgatacg cgcgcgttcc tatggcagag atgtgcacgt
     1921 ttggacggta aacgataccg agtcgatgct gcgctacaca agcatgggag tctcttccat
     1981 aatcacagac aggcccgaga cgctttcaca gcttctgatc agaaaagcac agctctcgat
     2041 atttcagaga agagtcctcg cggtcctctc attctaggat catcattacc cacaagttag
     2101 aagacgttct ggagtacggg aaggcgactg tgatagaata attttagagt ttgcagcggg
     2161 attggatgtg gggcttatgg gagtgtacgc ggtcgtgacg gccgatgttg tacgttcaag
     2221 agagcacgat ctctctctgg agattttgaa ggaaacgctt gagggtttcg ccagcgggct
     2281 tctggtgaag cctttttccg tatcgcgtgg cgatgagcta caggccgttc tctcagattt
     2341 agaagcgctc ccgatgcttt taaggcgttt gaggtattgt gtcctaccac tgcgtcttag
     2401 aataggcgta ggtattggtt tcatagagga ttctgctttt ctgaaggccg gaagctcctg
     2461 ggatatgagc gggaaggtct tcttcgatgc taggagcgct ctcgacgggg cgaaaaagtc
     2521 gaaagacgcg caaaccgttg tcgcctgcga agaagagaat ctcaggcttt ttcttaacac
     2581 cgttttcata ctcaaagacg cgatcgagag tgactggacc gaaaagcagt gggaagcagt
     2641 ccatacttac gagagagagg gaacttacga gagggccgcc acggctctcg gcgttacagc
     2701 gccgaatgtt cagaaccact gcgcaaaggc gaaatggaac gcgattaagt cggtggagac
     2761 caacatgtcc gcggctttgc gtgagaggtt ttccggtata taaatctata tagatttata
     2821 tattgaatat aaacctattt aagtttattt atgggcaata aaacccccgg gatttaaaag
     2881 gaggatcgat gccgctactt ttatcggtac ttgcacattt actggccgat ttcattttcc
     2941 agactgacaa gacggtagaa gacaggcttg cgcttcgctt ttccggtttc ttcaaacacg
     3001 ggataacggt ctttctgacg atgttagcgc tgttgcttcc ctacggcttt ataagtagct
     3061 ttttgtacag tctcgccgta tctttgattc acatcttcct cgatattatg aaaagctccc
     3121 tcgagaggcg gagaagcgcg gtcgcaagtt tctatctctt tcttatagac cagtttcttc
     3181 acatcgccac gataattctg ctcgttccgc tcttccactt aaaagtgacg gccagttttg
     3241 aaggtctgat cggcagtatg gccgcttata ctgggatcga tccaggcagg cttcccgtag
     3301 ggaggatgct cttcactgcg attgtctatc tttcggtggt ctttggaggc gctgtcttta
     3361 tacgaaagct tttcgacctg atatacagga acgagccgga ctatctctcg agaataatgg
     3421 ggagcaacgc cgtccttaac aacgtcagaa cgggtaaggt tatcgggatt ttcgagaggc
     3481 ttgtcgttct gactatctac ctcacgggca acgtggcgtc gataggtata gtgatcgccg
     3541 ccaaatcctt tgcgaggttc aaacacttcg ataacaagga tttcgccgaa tactatctca
     3601 taggaactct cgccagcgtc atgctcgcca taattggcgg cattattctc agaaccttct
     3661 gaacgtgtct gcctgccgtg ggtttttgcg gtggttatga ccggttaatc gtcgaacgcc
     3721 gatagatcgt tcctggaaaa cgggttcttc tttacacggt cgaggtattt caatctgaag
     3781 aggaagtata tcattccaaa gacttcccag acgatgaaga gcaggaagaa agaggtgctg
     3841 aaatctactc cgacctggta aagaagtata agcattatca ttattgaaaa tgccgtgccg
     3901 agaaccggta tgatgccgaa ccatttcttg ttcactatag ccggggcctt ttctttaagc
     3961 ctggggtctc tcttcaatct gaagacggca aacattgcag gaacaatgac cgcgatcccg
     4021 cctatcgaag cgaaggccgc gaagagattc agtccggatt cgccgaccga aatcagcatg
     4081 atcgaagata gaagccatac gattgtgagc agtacccagg gcgttcctct tctgttgacc
     4141 gctccaagtt tcgagggaaa gacgcgcagc ctgcagagcg caagtaaaga cctagttccc
     4201 cacatatatg tggcgtttat cgtggtggcg attgcaagaa tcggaccgcc gaacgcgaag
     4261 acataaaaaa gaaaaccgct cagataattc gaagcgacgt cgttcaacgt gccgttttcc
     4321 agcgtatctc cgccaacgcc gaacgagact acggccatca gaagatagca gatcagcaca
     4381 actgcaaggg agaagatcac cgagagcggg agattcttcc tggcgttctc cacctctccg
     4441 ccgagctcta tcacggcgtt tgaaccggca aaagtgaatg tgagaagcgc cgaagcatag
     4501 atcatcgaac ccattccggt cgagaaaacg gcttcaaggt ttttgctgtc gatacttccc
     4561 atgcccggta cggtgtaaac ggctatggca aggaacaaga gagccacgga aattatctcc
     4621 acacgagagg ccattttcag tcccaggagg ttgaccacgt agaagaaggt taaaatgatc
     4681 aaggccacgg gcacgagaga gaggtcattc cagatccctt tcatgtacga cgccagagcg
     4741 agcgcgttca gagggaaaag acctacaaaa gccccgaaga gatacgccca gaggcctaaa
     4801 aacgccactt ttggcgaaaa aaggaaggcg ccgtatttga aagttccccc cgtagttgga
     4861 agcgtcgagc cgagtaacat gacattgatc attatgaccg ccatcgggag gaaggcgaaa
     4921 acaaaagcca ttatagttcc cgctcccgca ctcctgtaag cgagcggcat atagacgaat
     4981 atcccggctc cgatcgtcat gccgatttct agagcgacga gatcccagaa cttgagggct
     5041 tttttcatgt tgagtacctt tccggccgat tgcgaggggc aacaaaacgg tccgagtttg
     5101 tatatcgtgt attatacaat atgcggctgc ccctgttata gccataccgt acccgttaca
     5161 gagcatggca aaaaaatggt acccttgaga tcgactattt tgcgccgtcc gacaggctca
     5221 gtctgtctgt atcgctttcc agttgaccgt tttgaataag gaactttatt tctttttcta
     5281 taagttcgtt catcgtggtt cccactctcc tgaagccgaa cagcttcgag acatcggtct
     5341 tcagatcctc cctgttcatg ctgaaggcat ctttcaggca gagtcttatc gcttccctga
     5401 cttcaacagg aggtatatga tcggccttcc ttgcgttcgc gcccgtcgct ctcctgggca
     5461 agaccgtttc gctacggttc aggatgaagt cgccttcgag ttcatacttt tccttcggaa
     5521 gattgaacag cagatattgc ttcaatctct ttctggagat gctacctgat tgcgtataca
     5581 tgttccggtt catcgcgggg aggagtgtcg ggtgattctc ataaagcccg agtattctgg
     5641 atacgacctc gtccagatgt accggcgcct ctttctccat cacagcctgt atcaactgtc
     5701 cggcactcga gagttttccg gatctcgaaa gctcgatcag atcaggcgga ttgaatctct
     5761 cataaaccgg caacgacggt ttatcgtttt cgagcttttg caggttctcc tccggtatgg
     5821 tttctacggt atttgattcg ccttcgacac ttgagttcga ccgggccttc tcgatctcgg
     5881 agaggagtct atccctttct gcctttctgt cgaataccca gtctctggac cagacccgcg
     5941 tgattttcca tccgaggttt tccagaactt tctggcggag tctgtctctg tcgcgggcgg
     6001 ttcttgccga atggtacatg gcgccgtcgc actctatccc cagcagatac tttccgggtc
     6061 tcctgtcgtc cacaacggcg agatctattc tgtaagagga acagccgacc tggctgtgaa
     6121 cagcgagaga gcattcggtc aactctcgat agacctcctc ttcgaatggt gaatcgaaat
     6181 cggcttccct gttcctcctt tcactctgaa gaaaccctgt atgaccgcca ctttctgcaa
     6241 aattgaggta ctccctcagg gccagcacac cgggcgtctg cacatcggcg ggaatatcgc
     6301 ccgcctttat cgagctcact accgttactt tctgtctcgc ccttgttatg gctacattca
     6361 accttctctc gcccccgttt ttgttgagag gaccgaagtt catggtgagc cttcccaaat
     6421 ggtctcttcc ataacctatg ctgaagatca tctcgtccct ctcgtcgccc tgaacatttt
     6481 cgaggttctt tacgaagaag ggttcaggag actcctcttt gaaaaactcc tcgagtgatg
     6541 gatcttctct tctgagcaat tcgagacagt ccattatagc catctgctgc gcctcgctga
     6601 aggcgaccac tccgagcgag accagcggct tgcttctggc atgattcatg accatcgtgg
     6661 cgaccctgag tgcctcttcc tgattctttc tgcttctggc cctatcgtag atgccgttat
     6721 ctacgaaggt catagaaatt ccacagccgg ggccgtcgaa attactggag ggaaaagtgt
     6781 tcaggcgatt ttcgtaaaaa tggtgattcg agaaggctat gagcgattca tgacggctcc
     6841 tgtaatgcca gagcagtttc ttttccatca atccggctgt gctgcactcg tccagtatgc
     6901 tctccagact ttctagatcg ctttcttcct cttcgagttc cgcaacgtcc ggctcggata
     6961 ttttgaagaa gctggtaggc gggagttgtt tattgtcgcc gacgatgact acctgatcgg
     7021 cccgcatgat cgccccgacg gagtcttcgg gaaagacctg cgacgcctcg tcgaagatca
     7081 ccagatcgaa tctaaatctt tccgggtcga tgaaaaccga gactgaaagt ggactcatca
     7141 tcatacaggg cttgaggact tgaagaaggt tgggtatctc ggagaagaga cgccttatgg
     7201 acttatttct tctcttcttg gcgacctctc ttttcagtat cgaggtctcg gcgcttcctg
     7261 acctggcaaa gtcgctgacg ggtatcttct gcgccagaag cgaagcgagt cgttttctgg
     7321 cgtagtgttg ctgcttgatg tccagccttc tgaattcctc cacgagtatg ttctgctcac
     7381 tccctgagaa tgaattgaga atcgtatctt ctccgtagaa gagtgaaagg agcctactgt
     7441 aaaaaccttt gaggaagacc tctcgcaggt cggccggatc tacaccttct tcgaaggcgt
     7501 tttccaggaa gtcctccagt ccg
//
LOCUS       DAOOLY010000108         7516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_11581_length_7516_cov_4.057097, whole genome shotgun sequence.
ACCESSION   DAOOLY010000108 DAOOLY010000000
VERSION     DAOOLY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11581_length_7516_cov_4.057097"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..931
                     /locus_tag="PLD15_06680"
     CDS             353..931
                     /locus_tag="PLD15_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPB63626.1"
                     /translation="MRKVVFSAVLVLLCSVVLASGLLMPVVKAPMIVTTLGQSPGALM
                     FRLVCVRSKIACVQEDLLTATQLKELAQAPDGPKTLVITTGTSLKGMGAAGIDINLEV
                     ERIENLIKQAKELGMTVIGAHIEGMARRVDATDEKSITTVIPKSDLILVIASSDEDGY
                     FTKFSETSGIPLLKVNESLDIGPALLELINAN"
     gene            991..2283
                     /locus_tag="PLD15_06685"
     CDS             991..2283
                     /locus_tag="PLD15_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPB63627.1"
                     /translation="MYFHAGLILAVIAVTFAVAKYFKLSVELSMLLAAIAAGIVHAGG
                     AIPVRHLVEGSFTYLDVCLIFITATFFMNLLKESGGVAFMVRKIVITFHKTRLLCLFF
                     LTVLLLIPGALTGSGAATVLTVGTLVGTVLSYMGISEKRVAAIIFICAAMSAAAPPVN
                     LWAMMAAAGSNMPYVGFFQPLLVLSAIGAFFAMFFLGAKGKNVELEKALKELPEPPEK
                     MNWLRVGIPFAVLIFLIMAGRIWPFSTPTIGLPLMFVLASVSAILLSPKKLQILRIAT
                     ETIHSLIPLVGIMIVVGILIQVMALSGARGLISLGVVTLPLWVLFSTLFLILPVSEGL
                     LQYAVAPLLGVPLILLFNMKGMNAIIALSAMAVMWPIGDILPPTAVVGRATVMELKFK
                     GHYYKDFLKTALIPMGFVLLVATLFLIFSNELSFLVGG"
     gene            2311..2475
                     /locus_tag="PLD15_06690"
     CDS             2311..2475
                     /locus_tag="PLD15_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63628.1"
                     /translation="MAILNSIISVCYYLISAFVVFVLAKSIIKTKNVQDAILYSVMLM
                     PFILRILRIK"
     gene            2488..3654
                     /locus_tag="PLD15_06695"
     CDS             2488..3654
                     /locus_tag="PLD15_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HPB63629.1"
                     /translation="MTDRNLLIVKLVSLALVAVALVIAGIQFHDHRNFQEEVVKGPGV
                     TQVKKLSDYFEPLKGSINDCNIYIIDSGVPGGTAFLIGSSHPEEPAGNLTAQLFVENA
                     KLAKGRLIVAIRANQSASRVSRPGDAYPLYYTIETPWGEKKFRMGDRWSSPLDSWPDP
                     EVYVNYPSGQLLAYMDVRNFNRTWPGRANGMITEQTNYAFMNVIKEEGVDIFIDLHEA
                     ELEYPVISTIVAHQKGADIAGMASMLLTSTEFRIGMEYSPKSLHGLSHREVGDHSDAV
                     SILLEAPEPFLDRVRGITDEKLLLTGQDEFVVTAGKYGLLYEKIDEKGWPIDVRVGRH
                     CSTVLMIFELWGDFNPGKEVVIQQVPRYDEVVQNGTGYYFRDPSKAKTEDIYYE"
     gene            3699..5282
                     /locus_tag="PLD15_06700"
     CDS             3699..5282
                     /locus_tag="PLD15_06700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB63630.1"
                     /translation="MKRVLITIILVLAFFYTGLMACTDIAVGKLATVDGSVISAQSVD
                     GSYDSRLLIQPAADHEPGSMTPVWEWIIYADRRPLVQLGEIPQVEHTYQWIQTSYPFS
                     NEKGLLIGETTIYGARATANGPDAIMTIEQLEAFALQRCTTAREAIELMGALAVEYGY
                     RESCARGEGLTIADGNEVWFFEVYGVGPLWKPGDGPGAIWAAQRVPDDHMTVIGNASI
                     IGEIDLTTNRPPNVPAEDFMICDNYLQSAIDLGLWTEGQEGPFVWKKVIGAIGSWNPR
                     VWRVFSKYQPSGNWEYGSDTSAYPFSFKPDTQVSVYDIIELYRDVMADTPADQLANEA
                     WNYVTNPGTDREQTRKSDLATPQPGSSIRNLIGLPSTQRLIAVQSTSCFFINQTREWM
                     PDTIGNVSWFGLGNGHKAVVVPVYCGITRVPDSWKNVNRGISKINRDEAFWAFYTVDR
                     LSQQRYGDMMPRIDAVRIPLQQKMYDMQEAIEATALALYETDPALANEFLTNYTNSLM
                     IEVERAYWDLADQLILRLP"
     gene            5340..6950
                     /locus_tag="PLD15_06705"
     CDS             5340..6950
                     /locus_tag="PLD15_06705"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB63631.1"
                     /translation="MNILRRALLILLVFLFTFGAMAMACTSILVGKAASVDGSTMTTH
                     TCDGSYDARIQIIPGGEHAEGETVSIYKGLCQAGIPGRTVSYVGEIPQAPVTYTYFHI
                     GYPFMNENQIIMGETTWSGKREIRNAQGWMTIEQLQVLGLQRAKTAREAIQIMGELAE
                     KYGYGDGGEGLTIIDGEEAWLFEIGGPGPFWSPGSEEPGAVWVAQRIPDDHVLVHANR
                     SRIGEIDLENTDYFMASPNVISLAVELNLWDPNSGEPFKFYAAYCDKTPFYNTRREWR
                     VLDLLAPSLELDPWAERYPLSVKPDHLVSAEELMMIKRDHYEGTEFDLTQGLAAGPFG
                     NPNRYATAANQRPAGVNYDGWERAISMFRCSYVIVGQARSWLPAAIGGVLWFGEDAPH
                     STVYIPFYAGNTSVPTSFSQGARDYFDRTSAWWAFNHVSNLADLRYDVAIKIIQAEAS
                     KFEKEFLANQPSIEKAALVLYEDNPALAIDFLTNYSNNAANRVVDRWWKLADELIYTL
                     NDGYVNGSTAGYPTWWLEAVGYGDTTRR"
     gene            7190..>7516
                     /locus_tag="PLD15_06710"
     CDS             7190..>7516
                     /locus_tag="PLD15_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63632.1"
                     /translation="MKRRDRGAISVAQAKRRRKAHNYLTLAGFLVGVLSVILIAVSHE
                     MSTSLVERADSIRLFFVFGILLGPVAYLLYLVSQIAALSNGIPRWNYLLCALAAIQVS
                     LSLFFAR"
BASE COUNT         1849 a         1981 c         1959 g         1727 t
ORIGIN      
        1 tttcaaccgc tttccccggt ccagataaag acggcgcgtc atatggccct aaatcgacga
       61 aaagtccatc atatcggaac taatcaacaa tatacgactt taatcgtttt tattcatcta
      121 taattgctcc tcaggcgatt acacaaacga aaagcgggat gatagaatta actgtaaacg
      181 ataagcatcc cgtacttatc attattattt accaatttat atttatatgg tctccacagc
      241 tgtttaaatg gctttctatc gtagtagcat ccgtattcga tgtttatcat cagttgaagt
      301 atgttcagcg cagttgcgcc ataattttgt gcttcagaaa gggaggtttt ttatgagaaa
      361 ggttgttttc tccgcggtac tggtacttct gtgttcagta gttctggcct ccggcctgtt
      421 gatgccggtg gtcaaagcgc ctatgatcgt caccactctc gggcagagtc caggagccct
      481 gatgttcaga ctggtatgtg taagaagcaa aatcgcgtgt gttcaggaag atcttctcac
      541 ggcgacccag ttgaaagaac tggcccaggc cccggatggc cccaagacgc tcgtcatcac
      601 caccggaact agcctcaagg gcatgggtgc ggccggaata gatatcaacc tggaagtaga
      661 gagaatcgaa aatctaatca agcaggcaaa agagctcggt atgactgtga tcggtgccca
      721 tatcgagggt atggcaaggc gtgtcgacgc caccgacgag aaatcgataa ccacggttat
      781 tcccaagtca gacctgatac tcgttatcgc ctcgagcgac gaggacggtt acttcacgaa
      841 gttctccgaa acgagcggaa taccgctgct caaggtaaac gaatcactgg atatcggccc
      901 ggcgttgcta gagctgatta acgccaactg acgacttaga ctttgatgac tcatcgatct
      961 ctcgatcggt attgaagggg gagggtacta gtgtacttcc acgctggtct catacttgca
     1021 gttatcgcgg tcaccttcgc cgtggccaaa tacttcaagt tgagtgtaga gctatccatg
     1081 cttcttgcgg caatcgccgc ggggatcgtc catgcgggtg gagccattcc tgttcgccat
     1141 ctggtggagg gttcctttac gtacctggat gtctgtttga tcttcatcac cgcaactttc
     1201 ttcatgaatc ttctgaagga gtccggtgga gttgcgttca tggtaagaaa gatcgtcata
     1261 acttttcata agacgagatt gctatgtctt ttcttcctga ccgtcctgtt gcttataccg
     1321 ggcgcgttga ccggttcggg agcggctacc gttctcacgg tcgggactct cgtcggaacc
     1381 gtcctgtcct acatggggat ctccgaaaag cgggtggccg caattatctt catctgtgcg
     1441 gccatgagcg cggcggctcc accggtcaat ctctgggcca tgatggccgc tgccggttcc
     1501 aacatgccgt acgtaggctt cttccagccg cttctagttc tatctgccat aggagcgttt
     1561 ttcgcgatgt tcttcctggg tgcgaaggga aagaacgtag agcttgaaaa ggccttgaag
     1621 gaactgccgg aaccgcctga aaagatgaac tggctgagag ttggaattcc cttcgcggtc
     1681 cttatattcc tcataatggc cggaaggata tggcccttct cgacgcccac catcggactg
     1741 ccgttgatgt tcgttctggc ttcggtgtcg gccatacttc tcagccccaa gaaactccag
     1801 atcctcagaa tagccactga aaccatacat tcccttatac cgcttgtcgg aattatgatc
     1861 gtcgtgggaa tcctcatcca ggtaatggct ctgagcggcg ccagaggtct tatatcgctt
     1921 ggagtagtaa ctctgccgct ctgggtgttg ttctcgactc tcttccttat actgcccgtt
     1981 tcagagggat tgttgcagta tgcagtcgca ccgcttctgg gtgttccgct catattgttg
     2041 ttcaatatga agggcatgaa tgcaataata gccctgtcag ccatggctgt aatgtggccg
     2101 ataggcgata tccttccacc taccgccgta gttggtcggg ccacggtcat ggaactgaaa
     2161 ttcaaagggc attactacaa agactttttg aaaaccgcgc tgattccaat ggggttcgtt
     2221 ttgcttgtcg caactctctt cttgatattc agcaacgaac tctccttctt ggtcggcggg
     2281 tgatgtcatg gctgaaagga agtgtttata atggcgatcc tgaactcgat aataagcgtc
     2341 tgctactacc tgataagcgc attcgtggtc ttcgttctgg ccaagagcat cataaagacc
     2401 aagaatgttc aagatgctat tctttactcc gtcatgctta tgcccttcat cctgcgaata
     2461 cttcgcataa agtagggggt gaagagtatg actgacagaa acctgttgat cgttaagctc
     2521 gtctcgctcg ccctcgtggc cgtcgccctc gtgatcgccg gaattcagtt ccacgatcac
     2581 aggaacttcc aggaggaagt ggtgaagggg cctggcgtaa cccaggtcaa gaaattgagc
     2641 gactatttcg aacctctaaa gggaagtatc aacgattgca acatatacat catcgatagc
     2701 ggtgtccccg gaggaactgc cttcctcatc ggaagctcac atcctgaaga acctgcaggc
     2761 aatctcacgg cccagctgtt cgtcgagaac gcgaagctgg cgaagggaag actgatcgta
     2821 gctatcaggg ctaaccagag cgcctctcgc gtctcaagac cgggagatgc ctatcccctc
     2881 tattacacca tcgaaacacc atggggagaa aagaaattca gaatgggaga ccgttggtcc
     2941 agcccgctag actcatggcc cgatcctgaa gtctatgtca actatcccag cggccagttg
     3001 ctggcctaca tggacgtcag aaacttcaac agaacgtggc cgggcagagc gaacggtatg
     3061 atcaccgaac aaaccaacta cgccttcatg aacgtaataa aggaagaggg cgtcgatatc
     3121 ttcatcgatc ttcatgaagc cgaactcgaa taccccgtca tcagcaccat agtcgctcac
     3181 cagaaaggtg ccgatatcgc cggtatggct tctatgttgc tcacctcgac cgaattcagg
     3241 atcggaatgg agtactcccc gaagtccctc cacggactct ctcacagaga ggtcggagac
     3301 cactcggatg ccgtctcgat cttgcttgaa gcgccggaac ctttcctcga cagagtgagg
     3361 gggatcaccg acgagaaact tcttctcacg ggacaggatg aattcgtagt gacggccggc
     3421 aaatacggtc tcctctacga gaagatcgat gaaaaaggct ggccgataga cgtcagagtt
     3481 ggaaggcatt gctcgacggt tttgatgatc tttgaactct ggggagattt caatccggga
     3541 aaggaggtag tgatccagca agtacccagg tacgacgagg tagtacagaa cggaacgggc
     3601 tactatttcc gtgatccgtc gaaagcaaaa acggaagata tttattacga atgaccggac
     3661 agattttggg taacacctca caaaggaggg gttaagtagt gaaaagggtt cttataacta
     3721 ttattcttgt attagcgttc ttctacacag gtttgatggc ctgtacagac atcgccgtag
     3781 gaaagctggc gacggttgac ggatcggtta tttccgccca gtccgttgac gggtcttacg
     3841 actccagact cctgatacag cccgccgccg accacgaacc gggaagcatg acacccgtct
     3901 gggaatggat aatctacgcc gacagaagac ctcttgttca gctgggcgag atcccccagg
     3961 tagaacacac ctaccagtgg attcagacat cctatccttt ctcgaacgaa aagggtcttt
     4021 tgatcgggga aacaactata tacggggcaa gagctacggc caacggtccg gatgctatca
     4081 tgaccatcga gcagctcgag gccttcgcac ttcagagatg tacaacggcc agagaagcca
     4141 tagagctcat gggagccctc gcagttgaat acggttaccg cgaatcctgc gcacgtggcg
     4201 aaggcttgac gattgccgac ggtaacgaag tatggttctt cgaagtgtat ggagtcggcc
     4261 cgttgtggaa accgggcgac ggtccaggcg cgatatgggc agcccagcgt gttcccgacg
     4321 atcatatgac cgtaataggc aacgccagca tcatcggtga gatcgatctt actaccaaca
     4381 gaccgccgaa cgttccggcg gaagacttca tgatctgtga caactacctg cagagcgcca
     4441 tcgatctcgg cctctggaca gagggtcagg aaggaccttt cgtatggaag aaagtaatag
     4501 gcgccatagg ctcttggaac ccgagagtct ggagagtctt ctccaagtac cagccgtcgg
     4561 gaaactggga atacggcagc gacacctccg catacccgtt ctccttcaag cccgacaccc
     4621 aggtctccgt atatgacatc atcgaactct accgcgatgt catggccgac acaccagccg
     4681 atcagctggc caatgaagcc tggaattacg tgaccaaccc cggaaccgat agagagcaga
     4741 ccagaaagag cgatctggca acacctcagc ccggtagctc tataagaaac ctcatcggac
     4801 tgccgtccac acagaggctc atcgccgtcc agagcaccag ctgcttcttc atcaaccaga
     4861 ccagagaatg gatgcctgac acaataggta acgtttcctg gttcggtctc ggcaacggcc
     4921 acaaagccgt cgttgttcct gtgtactgtg gaataaccag agtacccgat tcctggaaga
     4981 acgtcaacag aggcatctcc aagatcaaca gagacgaagc tttctgggcc ttctacaccg
     5041 tcgatagact ctcccaacag aggtacggcg atatgatgcc gagaatcgac gccgtaagga
     5101 ttccgttgca gcagaaaatg tacgacatgc aggaagccat cgaggcaacc gcgctggcac
     5161 tgtatgaaac cgatcccgca ctggccaacg agttcctgac caactacacc aactctctga
     5221 tgatcgaagt cgagagggcg tactgggacc tcgcggatca gttaatactc agattaccgt
     5281 aatatctttg gaccatattt cgggccggtt acttgaccgg cccacttttc gaaaagggag
     5341 tgaacatttt gagacgagct ttattgattc tactggtgtt tcttttcact tttggagcca
     5401 tggcaatggc atgtacctct atcctggtgg gtaaggctgc ctccgtcgat ggctcgacga
     5461 tgaccacgca cacctgcgac ggcagttacg acgcgagaat ccagataatc cccggcggcg
     5521 agcacgccga aggtgaaaca gtcagcattt acaagggtct ctgccaggcc ggtattcccg
     5581 gaagaaccgt cagctacgtc ggcgagatac cccaggcgcc cgtgacctac acctacttcc
     5641 atatcggtta ccccttcatg aacgagaacc agataattat gggcgaaacc acatggagtg
     5701 gaaagagaga aataagaaac gctcagggat ggatgacgat cgagcagctc caggtcctcg
     5761 gactccagag agcgaagaca gctagggaag ccatccagat catgggtgaa ctcgccgaga
     5821 agtacggtta cggagacggc ggcgaaggcc tcacgatcat cgacggcgaa gaagcctggt
     5881 tgttcgaaat aggcggcccc ggcccattct ggtctcccgg cagcgaagag cccggagcgg
     5941 tatgggtagc ccagagaatc cccgacgatc acgttctggt gcatgccaac agatccagaa
     6001 taggtgagat cgatctggaa aataccgatt acttcatggc ctctccaaac gtcatatcgc
     6061 tggccgtaga gctcaacctt tgggacccca acagcggcga gcctttcaag ttctacgcgg
     6121 cttactgcga caagacgcct ttctacaaca cgcgccgtga gtggagagtc ctcgaccttc
     6181 tggcaccttc tctggagctc gatccgtggg cggagagata cccgctgtcc gtgaagcccg
     6241 atcacctggt ttccgccgag gaactcatga tgatcaagcg cgaccactac gaaggaacgg
     6301 aattcgacct gactcaggga cttgcggccg gacctttcgg caaccccaac agatacgcca
     6361 cggcggccaa ccagagacct gcgggcgtaa actacgacgg atgggaaaga gcgatctcca
     6421 tgttcagatg ctcctacgtt atagtcggtc aggccagaag ctggctgcca gcggcgatcg
     6481 gcggagtact ctggttcggt gaagacgcac cccactccac agtttacatc ccgttctacg
     6541 cgggcaacac gagcgttccg acctctttca gccagggcgc gcgcgactac ttcgacagaa
     6601 catctgcatg gtgggccttc aaccacgttt ccaacctggc cgacctgaga tacgacgttg
     6661 cgatcaagat aatacaggcc gaagccagca agttcgagaa ggaattcctg gccaatcagc
     6721 ccagcatcga aaaggcagcc ctcgtgctgt acgaagacaa cccggctctg gctatcgact
     6781 tcctcacgaa ctacagcaac aacgcggcca acagagttgt cgacagatgg tggaagcttg
     6841 cagacgaact gatatacacc ctcaacgacg gttacgtcaa cggttcgacg gcaggttacc
     6901 cgacctggtg gctcgaagcg gtcggctacg gcgatacgac cagaagataa ttcacgtttt
     6961 tctacaaaaa gggcgggctt tccgcccttt tttcttttta taaaacgacc tgtccggctt
     7021 ttgaagggag atatgtactt ccagaatatt aaatacgaca tatgttctaa acagtagtgt
     7081 tgcttttaag agcgattaga attagaatat agatatgcgg cttttcagta cacccgtgtg
     7141 gcggggcgat ctgtcgcgga tttgattatt ctgtggtctg gtgattatgt tgaagagaag
     7201 agatagggga gcgatcagcg tcgctcaggc gaagcgccgg cggaaggctc ataattatct
     7261 gactctggcg ggctttttgg ttggtgtttt atcggtgatc ttgatagccg tctcgcacga
     7321 gatgtcaacc agtctcgtag aaagggccga ctcaataaga ctgttcttcg tcttcgggat
     7381 actcctcggg ccggtcgctt atctgctgta cctggtctcg caaatcgccg ccctctccaa
     7441 cggaataccg cgatggaatt atcttctgtg cgctctggcc gccatacagg tctcgctctc
     7501 gctcttcttc gccaga
//
LOCUS       DAOOLY010000109         7451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_11749_length_7451_cov_5.384667, whole genome shotgun sequence.
ACCESSION   DAOOLY010000109 DAOOLY010000000
VERSION     DAOOLY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11749_length_7451_cov_5.384667"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1195
                     /locus_tag="PLD15_06715"
     CDS             125..1195
                     /locus_tag="PLD15_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPB63633.1"
                     /translation="MKKACVIGVVFALGIALLNSGCIPLEKLYGYSAWVVGDVAKDGK
                     AMILYSDDSGGSWSRQGTDVLPDGENLSDVYAVSMYEVWAVGSGGLLIKTNNGGKNWK
                     VVDLPDELKAGELAHISIFEDDIWLSGNEGLIGFSKDGGKSWTVADLPEGSQEYLLQG
                     IHAINNDLVYAVGNKSTGTETGIVLRTEDGGLTWDRIVLPNDYNVNGWIGVKATDENH
                     VIIFGGKGHYAVTANGGKQWVAGGPLFWKDLNSLVMLDERTYWAACDFDTIILTRDSG
                     ISWEEQPSAGTGNSFLLGIAALDRNHALIAGGSAGYPQFGKILRTSNGGQSWEVVLDG
                     DECDVPLWHVAIAKHNYMAPLP"
     gene            1316..2701
                     /locus_tag="PLD15_06720"
     CDS             1316..2701
                     /locus_tag="PLD15_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB63634.1"
                     /translation="MLSDEAYSKIINRILSGGADFAEVFVEERYTNNISMIQGEIEDS
                     SSGKLLGAGLRAFKGNRSIYAYTNDVSFEGLMDVAGRLKAVVGQEIDRDLALDFRAGE
                     HKDICPILLMPNSVSKKDKAAVMRLAHEGASGFSGRISQVVVNYFDYSQNVWIYNSEG
                     VKASDSRVRTRLMVSAVASVNGDKEVGFYGPGAAQGFELFNRIDPRAAGERAARIADR
                     MIRAEYAPAGKMPVIISNEFGGVIFHEACGHALEATSVAKGASVFAGKLGEKVAGECV
                     SAVDDPTIPNAWGSANVDDEGTPTRRNLLIEKGILKSYMIDRLGGLKMKMEPTGSSRR
                     QNYTFAPTSRMSNTFILPGDHYPEEIIAATDYGLYARSMGGGSVMPATGEFNFAVNEG
                     YLIEKGRITKPVRGATLIGKGNEVLMKIDMVGNDLARGQGMCGSISGSIPADVGQPTL
                     RVSELIVGGRS"
     gene            2701..4044
                     /locus_tag="PLD15_06725"
     CDS             2701..4044
                     /locus_tag="PLD15_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB63635.1"
                     /translation="MEWKKFKDTVFALAKKKGFDEAEIYYSSGSEFQLSSLRGEIDSY
                     NDATSIGVYFRGLKSGKIGAAFAEELTDETASMLVDEAFENYSIASGNDVYFLHDGSG
                     EYPLFEGFSGAFSAQPVEEKIERVIALERSALEYDKRIIMAPTCRLGDSRSEVFIVNT
                     LGLDRHYSSDGGVAVVVALAGEGNSKKTGFTFTIAREPGDIDVSALGKEASKRAIDQL
                     GAGRVKSGKYRVLFRNDMFGQLFATFTSMYSAENVQKGLSIIADKEGTKIASELLTVY
                     DDPLLPISPNSRPFDDEGVPTVKKTLVEKGLLKTFLYDLKTAAKAGTKSTGNAIKSSY
                     RSKPSISMINTVVKPGEKSFNTLVGELDEGIVVTELTGLHSGANPISGEFSLGASGFY
                     VKNGSIVNPVEQFTISSSILKVYNSIVAVGSDAMPSIFRVSSPSVMVSEIDVASE"
     gene            4062..4265
                     /locus_tag="PLD15_06730"
     CDS             4062..4265
                     /locus_tag="PLD15_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB63636.1"
                     /translation="MPLYRFVCDKCGREESLLLKMSAPVPPCGGCGGALVKQITNISS
                     YGCSNGSESCGSCSGGSCSSCGH"
     gene            complement(4372..5697)
                     /gene="glnA"
                     /locus_tag="PLD15_06735"
     CDS             complement(4372..5697)
                     /gene="glnA"
                     /locus_tag="PLD15_06735"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPB63637.1"
                     /translation="MTVEKLIQEAKSGEIKFVRLQITDINGMLKNVEVPSKMLPQILE
                     KGAMFDGSSIDGFVRIEESDMYLKPDVNTYATLPWDGNQGKTVRLICDVFKPDGSPFE
                     GDPRYVLKKVIERAWKLGYEAFAGPEPEFFIIKREKDCVGPNGRFLDMGSYFDLLPVD
                     GGEEVRKKIVLSLQEMGFEVEAAHHEVAPSQHEIDFRYTDILKTADNIQTFKWVVKTI
                     SIVNGLHATFMPKPFEGVAGSGMHVHLSLFSKGRNAFYDPESEHGLSEVALNFIGGII
                     AHAKEITAITNPTINSYKRLLPGFEAPVNISWALGNRSAMIRIPSAREEATRLEFRSP
                     DPTCNPYLALALALAAGLDGIENKITPPNPVDENIFDMKGSRKKQLMIDNLPQSLWHA
                     LEYTKVSEFVKGVLGQHIFSTFIRARESDWSEFGAAVTSWEVKKYMDLY"
     gene            5849..7273
                     /locus_tag="PLD15_06740"
     CDS             5849..7273
                     /locus_tag="PLD15_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HPB63638.1"
                     /translation="MNIKRAFISVHDKTDIEKLGRYLAKNGVEIVATDHTAEYLDSFG
                     VRVSKVGDYTGFPPVFDGRLKSLHPKVIGGVLAIQDSKIHQGEMESNGILPFDMVVVN
                     LKPFDLAVEESPEESNLSKEIDVSGPALLMAAAKNHRDVVAISDRGDYDEIIESLEFC
                     GDVLLLKRRRYALKAIYSVMLYVSSIHRALSQIFASEKYDYTIMEHIMPLLYGDNPSQ
                     KANLLKLSVEPGLLDEVQIGNPSVGLKKSQLRNINVFMELYYYTDNISVFLDKGKITK
                     VSSAMVPGELNFGGPDTMFASTYAISDELMVALRERGVEAFGGVFDLEAIRTARMNDV
                     MVLTIPEREAVSLSGEAYSGFGNYFVKEESFKMSILDLQPDEKLALFCSRVNRANNVS
                     VFRDGESLLLKGGLSFKELNELDFSKYDLKGSTFASNQDLDDDLIDKLIGAGVWKIIL
                     PATKRSYSRDAIVQLGYDFTRLNN"
     gene            7319..>7451
                     /gene="ffs"
                     /locus_tag="PLD15_06745"
     ncRNA           7319..>7451
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD15_06745"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         1686 a         1869 c         2229 g         1667 t
ORIGIN      
        1 ttccagcctc tcgcctctta cctctagcct caggttctcc aaggacggct ctttctgctc
       61 tttccgcccc tcgatagagt agaatttctt cgtgggaagt tcaccgcaaa agggagggtt
      121 ttcaatgaag aaagcatgcg tgataggggt ggttttcgct ctgggcatcg ctcttttgaa
      181 cagcggttgt atccccctcg aaaagctgta tggctactcc gcctgggtcg tcggggacgt
      241 agcgaaggac ggcaaggcta tgatactgta ttcggacgat tccggcggga gctggagccg
      301 tcaggggacg gacgttctcc ccgacgggga gaacctgtcg gacgtgtacg ccgtgagcat
      361 gtacgaggtc tgggccgtcg ggagcggagg tctgttgatc aagacgaaca acggcgggaa
      421 aaactggaaa gtagtcgatt tacccgatga gctcaaagct ggcgagttgg cgcacatttc
      481 gatattcgag gacgatatct ggctgagcgg gaacgaggga ttgatagggt tttcgaaaga
      541 tggaggcaaa agctggaccg tggccgatct gcccgaaggc tctcaggagt atcttcttca
      601 gggaatacac gcgataaata acgatctcgt ctacgccgtg ggaaacaagt ccacggggac
      661 cgagaccgga atcgttctcc gcaccgaaga cggcggtctt acctgggata gaatagtcct
      721 tccgaatgac tacaacgtga acggctggat aggagtcaag gcgaccgacg aaaaccacgt
      781 gataattttc ggcggaaagg gacattacgc agtgaccgcc aacggaggga aacagtgggt
      841 cgccggcggg ccgctcttct ggaaggatct caactctctg gtgatgctcg acgagaggac
      901 ctactgggcg gcctgcgatt tcgacacgat aatactcacc agagacagcg gcataagctg
      961 ggaggaacag ccgtcggccg gaacgggcaa ctcttttttg ctcgggatag ccgcgctgga
     1021 tagaaatcac gcactcatag cgggtggctc tgcaggatac ccgcagttcg gcaagatact
     1081 gcgaactagc aacggcgggc agagctggga ggtcgtcctg gatggcgacg agtgtgatgt
     1141 ccctctatgg cacgtggcga tagccaagca caattacatg gcccctttgc cttgattgct
     1201 gggcgaagtc gagagccggt ctttcaatag cgaggaatag tatcttcttt tgtctccggt
     1261 tgagtcgctt tcatgttgta gaatagtaag aataacaaac aaaaggaggt gctgaatgct
     1321 ttcagacgaa gcctattcga agataataaa ccgtattctc tccggcggag ccgatttcgc
     1381 cgaggtgttc gtggaggaga gatacaccaa caacatcagt atgatccagg gagagatcga
     1441 ggacagcagt tcgggaaagc ttctcggagc tggattgcgg gctttcaaag gaaacaggag
     1501 catttatgcc tacaccaacg atgtgagttt cgagggtctt atggacgtgg ccggcaggct
     1561 gaaggcggtg gtgggacagg agatcgacag ggatctggct ctcgatttca gggccggtga
     1621 acacaaagat atctgcccga ttctcttgat gcccaacagt gtctcgaaaa aagacaaggc
     1681 cgcggtcatg agactggcgc acgagggggc gtcgggtttt tccggcagga tctcgcaggt
     1741 cgtcgtgaat tacttcgatt actcccagaa cgtctggata tacaattccg aaggcgtgaa
     1801 ggccagcgac agccgtgtca gaacgaggct catggtttcg gccgtggcga gcgtgaacgg
     1861 ggacaaggaa gtcggtttct acggtcccgg cgcggcgcag ggcttcgagc ttttcaacag
     1921 gatagatccc cgcgcggccg gagagagggc ggcaagaata gcagaccgca tgatccgggc
     1981 cgaatacgcc ccggccggca agatgcccgt gataatctcc aacgagttcg gcggcgtcat
     2041 tttccacgaa gcctgcggcc acgcgctgga agccacaagc gtcgccaagg gagcctccgt
     2101 attcgcgggg aaactcgggg agaaggtagc gggtgaatgc gtctcggccg tcgacgaccc
     2161 gacgattccc aacgcctggg gttcggccaa cgttgacgac gagggaacgc ccaccaggag
     2221 aaacctcctg atcgagaagg ggattctcaa gagctacatg atcgacaggc tgggcggtct
     2281 gaagatgaaa atggagccca ccgggagctc tcgaaggcag aactacactt ttgctccgac
     2341 ttcgaggatg agcaacactt tcatactgcc gggcgatcat tatccggagg agataatagc
     2401 cgcgacagat tacggacttt acgccaggtc catgggcggc ggttcggtga tgccggcgac
     2461 gggcgagttc aacttcgcgg tgaacgaggg ttatctgatc gagaagggga ggataactaa
     2521 acccgttcgc ggcgcgactc tgatcggtaa gggcaacgag gtgctcatga agatcgatat
     2581 ggtgggaaac gatctggcca ggggccaggg aatgtgcggt tcgatctcgg gctccattcc
     2641 ggccgatgtc gggcagccga cgctcagggt atctgaattg atcgttgggg ggcgaagctg
     2701 atggagtgga agaaattcaa agatactgta tttgcgctgg ccaaaaagaa gggtttcgac
     2761 gaggccgaaa tctactactc cagcggtagc gaattccagc tcagttccct gagaggcgag
     2821 atagacagct acaacgacgc cacctctatt ggggtctatt tcagaggcct gaaatcggga
     2881 aagatcggcg cggccttcgc cgaggaactg accgatgaga ccgcctccat gctcgtggac
     2941 gaggccttcg aaaactactc tatcgcgagc ggcaacgacg tgtactttct ccacgacggg
     3001 tcgggggagt acccgctgtt tgaaggcttc agcggcgcct tttccgccca gccggtcgag
     3061 gagaagatag agagggtaat tgcgctggag cgaagcgctc tcgagtacga caaaaggata
     3121 atcatggcgc ccacgtgccg gcttggagac agccggtcgg aggttttcat cgtcaatacg
     3181 ctggggctcg acagacatta ctcgtccgat ggcggggtcg ccgtcgtcgt ggctctcgcc
     3241 ggcgagggta attcgaaaaa gaccggcttc accttcacga tcgccagaga gcccggagat
     3301 atagacgttt cggcgctggg caaagaggcc tcgaaaaggg cgatagacca gctcggagcc
     3361 gggagggtca agagcggaaa gtacagggtg ctgttcagaa acgatatgtt cggccagctc
     3421 ttcgcgacct tcacgagtat gtattcggcc gagaacgttc aaaaagggct ctccataatc
     3481 gcggacaaag aaggtacgaa gatcgcctcg gaactgctca ccgtttacga cgatcccctc
     3541 ctgccgatca gcccgaacag ccggcccttc gacgacgagg gcgtaccgac ggtgaagaag
     3601 acgctggtcg agaagggcct tctgaagacc ttcctttacg atctgaagac ggccgccaaa
     3661 gccggaacca aatccaccgg caacgctatc aagtcgagtt acaggtcgaa gccttcgatc
     3721 tccatgataa acaccgtcgt taagcccggg gaaaagagct tcaacacgct ggtcggagag
     3781 ctggacgagg gaatagtggt gaccgagctc acgggcctcc attcgggagc caaccctata
     3841 tccggcgagt tctctcttgg cgcgagcggg ttttacgtga agaacggttc gatcgtcaat
     3901 cccgtcgagc agttcactat ttcctccagc attttgaagg tctataatag tatcgtagcc
     3961 gttggaagcg atgcgatgcc ctctatcttc agggtgagct ctccctccgt gatggtatcg
     4021 gagatagatg tcgcttccga atagatctgg aggtgttttc tttgcccttg tacagattcg
     4081 tctgtgataa atgcggacga gaggaaagtc tcttgctgaa aatgagcgcg cccgttcccc
     4141 cgtgcggcgg ttgcggaggc gctctggtca aacagataac caatatatcc agctacggct
     4201 gctcaaacgg atcggagagc tgcggctcct gctccggcgg aagctgttcc agctgcggcc
     4261 attgagagac tgcgatacat atctcgaaac ggtaataaaa aagatgccgg agttgccggc
     4321 atctttttta ctggtagtct atgattcggt cgggctcttt tacggccggt ttcagtagag
     4381 gtccatgtat tttttaactt cccagctggt gacggccgcg ccgaattcgc tccagtcgct
     4441 ctctctggcc cttatgaagg tgctaaagat gtgctggccg agaacccctt tcacgaattc
     4501 gctgaccttt gtgtattcga gggcgtgcca gaggctctgg ggaaggttgt ctatcatgag
     4561 ctgtttcttt ctggagcctt tcatgtcgaa tatgttttca tcgacagggt tgggcggtgt
     4621 gatcttgttc tctattccgt ccagtccggc cgccagtgcc agcgccaggg ccagataggg
     4681 attgcaggtg gggtcggggc ttctgaattc gagcctggtg gcctcttctc tggcgctggg
     4741 tatcctgatc atcgcgctcc tgtttccgag ggcccaggat atgtttacgg gggcctcgaa
     4801 accggggaga agtcgcttgt agctgttgat ggtagggttg gttatcgcgg tgatctcttt
     4861 tgcgtgggcg attattccgc ctatgaagtt cagggccacc tcgctcagcc cgtgctcgct
     4921 ttcggggtcg tagaaggcgt ttctcccttt cgagaagagg ctgaggtgga cgtgcatccc
     4981 agagccggcg acgccctcga agggtttggg catgaaggtg gcgtgcaggc cgttgactat
     5041 ggatatcgtc ttcaccaccc atttgaaagt ctgaatgttg tcggccgtct tcagtatatc
     5101 cgtgtacctg aaatcgatct cgtgctggga gggcgccact tcgtggtggg ccgcctcgac
     5161 ttcgaagccc atctcctgca gagagagaac gatctttttc ctcacttcct ctccgccgtc
     5221 taccggaagg agatcgaagt aactccccat gtccagaaat ctgccgttgg gtccgacaca
     5281 atctttttct cttttgatga tgaaaaactc gggctcggga ccggcgaagg cctcatagcc
     5341 gagtttccaa gccctttcga ttaccttttt gagaacgtat ctgggatcgc cttcgaaggg
     5401 cgatccgtcg ggtttgaaga cgtcgcagat caaccggacg gtctttccct ggtttccgtc
     5461 ccagggaagc gttgcgtagg tgtttacgtc gggtttgagg tacatgtccg actcttcgat
     5521 ccggacgaat ccgtctatcg aggagccgtc gaacatcgcg cccttttcca gaatctgcgg
     5581 gagcattttc gatgggactt ccacgttctt cagcataccg tttatatcgg ttatctgaag
     5641 ccttacgaat ttgatttctc ccgactttgc ctcctgaatc aatttctcga cagtcattgt
     5701 tcccccctta tatacccggc ccggtgggta tgagatactt cagtatagta gtattaagct
     5761 aacggcgaaa taagcgaaca tgaaagttct acgggaaata tgatgtaaac ttgtctcatc
     5821 tggaaaattt cgatggaggt gtcgaggagt gaacataaag cgcgcattca taagcgttca
     5881 cgataagact gacatagaga agctcggccg gtatctggcg aagaacggtg tggagatagt
     5941 cgcgaccgat cacacggccg agtacctgga ttctttcggg gtcagagtgt ccaaagttgg
     6001 cgattacaca ggtttcccac cagttttcga tggcaggttg aagtctttgc atccaaaagt
     6061 gatcggtggg gtcctggcga ttcaggattc gaagatccac cagggagaga tggagagcaa
     6121 cggtattctc cctttcgata tggtcgtggt gaacctgaag cccttcgatc ttgccgtcga
     6181 ggagtcgccc gaggagtcga acctttccaa agagatagat gtcagcggcc cggccttgct
     6241 tatggcggcc gctaagaatc acagggacgt tgtggcgatc tccgatcgcg gcgattacga
     6301 cgagataata gagagcctcg agttctgcgg cgacgtgctg cttttgaaga gacgcagata
     6361 cgcgctcaag gccatctatt cggtgatgct ttacgtatct tccattcaca gggcgctctc
     6421 gcagatattc gcctccgaga agtacgacta tacgataatg gagcatataa tgcccctgct
     6481 ttacggcgac aacccttcgc agaaggccaa tctgctgaag ctttccgtcg agccggggct
     6541 gctcgacgag gtacagatcg gcaacccttc ggtcgggttg aagaagtctc agctgagaaa
     6601 tataaacgtc ttcatggagc tctattacta cacagacaat atatcggtct ttctggacaa
     6661 ggggaagatc acgaaggtat cgtccgcgat ggtgccgggc gagctgaatt tcgggggacc
     6721 ggacactatg ttcgcctcga cctacgcgat aagcgacgag ctgatggtcg cccttcgcga
     6781 gcgcggcgtg gaggccttcg gaggcgtttt cgatctggaa gccataagga ccgctcgaat
     6841 gaacgacgtg atggtactga ccataccgga gagagaggcc gtttctctgt cgggggaggc
     6901 atattccggt ttcgggaatt acttcgtcaa ggaggagtcc ttcaaaatga gtatcctcga
     6961 ccttcaaccc gacgagaaac tcgcgctttt ctgctctcgc gtgaaccggg ccaacaacgt
     7021 ctccgttttc agggatgggg agagtctcct cctgaagggc gggctcagct tcaaagagtt
     7081 gaacgaactg gatttttcca aatacgatct aaagggttcc acgttcgcct ccaaccagga
     7141 tctggacgat gatctcatcg ataagttgat cggggcagga gtctggaaga tcattctacc
     7201 ggcgacaaag aggagctatt ccagagatgc gatagtccag ctcggttacg acttcaccag
     7261 attaaacaat tgatgagcac ttgaaagctg gcccgacaga atatataatg tatttacacc
     7321 atgctaggcg gggagctggc ggttccctgc acccgaaatc cgccatacgc ggggccgaaa
     7381 gccctccgag gctttgtcgg tttggtgtcg gtttgtctaa aggtgttgag gattgagtcc
     7441 tgcgcaacgg g
//
LOCUS       DAOOLY010000110         7258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12162_length_7258_cov_4.547411, whole genome shotgun sequence.
ACCESSION   DAOOLY010000110 DAOOLY010000000
VERSION     DAOOLY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12162_length_7258_cov_4.547411"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2312
                     /locus_tag="PLD15_06750"
     CDS             <1..2312
                     /locus_tag="PLD15_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB63639.1"
                     /translation="IDSDLMKILPENDPVIRQYESFIEGNSSRDVTYIVIKSDDKSPG
                     GIDNLVAAAREIFDSADQFSSIEAFVRFDVISDLGPLGFVLIDSSELESLSGSNQDMV
                     RNVQRLVNYDFSAIHSIGEMLAGFDGFKDMLKEDESAGRFENFIRLPESIPQKDPMIL
                     VMGFKLDGPSSDVAYVARVIPALRKWISEKLEPYDLTYGMAGDHFGTYDSHRQATDDF
                     MLTTVISLTGISLLFLLAYSSFKITLFVFSSLAVSMFITLGAAYLIFGSLNIITSFVT
                     SITLGLGIDYGIHMITRLSDGAKKERNAQRLLREAYKSLLKPLMASMLTTAAVFVILA
                     LIDSPAARELGILTSFGIVIFFIVMITFLPALSIKTVMKQGRSANIHLLDRFFLRLAR
                     LIRKLGVVSGGIVFILILSLGYLGYISITNFSYTPPGLMATDSEQIAVPALIERTFGG
                     SILNSVPFMVGDVESLRRAHENLNNNPNIRSVFSVLSILEDGSQDQVNRIQSITRQIN
                     SMRNSPLVEALLKKAQYYDFMIELLDKAEGVKSSDELIDLIAEVIPDSLKERVLYEDG
                     QGKRYFVINAEPTSIIYRNNVIKILFDSVGEELREAAGGYPKVFYYLMDVVRKISIPV
                     SLFAFVIVFLIVTIERKSLFDGLKLFALMVGILMTMFGLMEISGIETTFITVISAPLI
                     IGIGVDALVYVVHSSGKRDEKELARTLKSITMSSATTMLAFFSFAFAEGKLLSSFGLS
                     LASGVLMSLVVATFLVPVLPWNSKERNGG"
     gene            2316..2696
                     /locus_tag="PLD15_06755"
     CDS             2316..2696
                     /locus_tag="PLD15_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63640.1"
                     /translation="MPFHLLVAYYVVDHAFVNNTKLSKMDDKKLWTHFIWVVLVFLAF
                     TFDVFLSNPLGILLLILSIGVTVTLDMGRKKLSNHLIEIIAFFIFLFFTLLGKSYLSR
                     SFVTIEFPGIFWGCLPQPWASRIF"
     gene            2708..2932
                     /locus_tag="PLD15_06760"
     CDS             2708..2932
                     /locus_tag="PLD15_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63641.1"
                     /translation="MAENATDSIGIAERMSIFIFVLAGHWEWTLIAIGAALTFRVVFS
                     KDGKIEWLISPVAGVGLSFLWQLLMRSMLG"
     gene            2929..3546
                     /locus_tag="PLD15_06765"
     CDS             2929..3546
                     /locus_tag="PLD15_06765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB63642.1"
                     /translation="MTYAVGDIHGCLDVLRRLVQSLEAKSSDVFVFIGDYVDRGPDSK
                     GVIDYLIALSERYECHFIRGNHEQMFLDYLRREPGAMIWAYNGMERTLMSYGSLRAVP
                     DCHIDFLNRTVYSYTIKKFVFVHAGVRPGIPLEEQEERDLLWIREPFLSNRNPIKGKT
                     VIFGHTPQFGGPLIEKSKIGIDTGCVYGGYLTAYRIEDRHWVCEK"
     gene            complement(3594..4847)
                     /locus_tag="PLD15_06770"
     CDS             complement(3594..4847)
                     /locus_tag="PLD15_06770"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPB63643.1"
                     /translation="MAEISLFENALKQFNKAADVMKLSSSMRQVLTSTKRELTVNFPV
                     RMDNGEIKVFTGHRVQHNVARGPAKGGIRYHQNVTLDEVKALAFWMTWKCAVVGIPYG
                     GGKGGVCVNPQELSMGELERLSRRYFSEIQIIIGENEDIPAPDVNTNAQIMSWYMDTY
                     SMNVGHSVLGIVTGKPLEIGGSKGRPEATGRGVRVIAEEAAKYKGMDPKNATFAVQGF
                     GNVGSFAAKLISEEIGSKVVALSDVSGGLYNPAGFDIDEVIAYRDQNKGVIEGYPKGQ
                     KISNNDLLTLDVDILVPAALENAITANNAGDIKAKIIVEGANGPITPEADEIILSKNI
                     FIVPDFLANAGGVTVSYFEWVQGLQHYFWDIEDVRRALHKIMKEAFNSVASTANKYDI
                     DMRSAAYVDAIEKVALATKLRGIYP"
     gene            complement(5040..5114)
                     /locus_tag="PLD15_06775"
     tRNA            complement(5040..5114)
                     /locus_tag="PLD15_06775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5079..5081),aa:Arg,seq:cct)
     gene            5237..6094
                     /locus_tag="PLD15_06780"
     CDS             5237..6094
                     /locus_tag="PLD15_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPB63644.1"
                     /translation="MERIVALESTVIAHGLPFPENVNTALEMERAVRENGGLPKTIGI
                     IDGDVRIGLQKEEILSLAKASDVMKVGTAEVAYATALKRKAATTVSATMFLARLHGIS
                     VFSTGGIGGVHRDIDWDVSQDIIELSRNDIIVVSAGVKSILDVQKTLEFLETFQVTVL
                     GYRTDYFPLFHCRDSQYKLNLRVDSPEEVVEVLKEKRRLGIKGGILVANPIPEESAIG
                     LDEINHLVDQAVSLARKEGVGGKALTPYLLSKIGFLSDGKTLRANISLLINNAALGGK
                     IAASQAWVV"
     gene            6091..>7258
                     /locus_tag="PLD15_06785"
     CDS             6091..>7258
                     /locus_tag="PLD15_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB63645.1"
                     /translation="MNYILKNSRVYSVDTGEFSDRDLYITDGRISDRAGPESLTMDLS
                     GKFIYPGFVDSHAHLIGTGLEKMVVDLRNCRSMEDLRDRLQIESHLVRARGWDQETLG
                     FLPERRILDRITSQPAVLVRRCGHVATVNSAVIDRFSLEALDGLDETDIETGVIKERA
                     LERLNSIVKLAPEEVEEAIRRGSVEFLKYGVTCVHSDDLHGMELETLVESLKRIDNIR
                     VFEKIAVERPDQLSMVSMARNGETRFFKVIAAKIYLDGSLGARTAAMKEPYSDSPRET
                     GVLYMDCETLKSIVERADREGIQLCIHVIGDRSLEEALKAFHGSRALELKHRLIHVQI
                     ASPEEIHRIADLGLTASIQPVFFDSDAHIAPGRFGEARIKNAYPFRQMRDAGIRM"
BASE COUNT         1836 a         1674 c         1832 g         1916 t
ORIGIN      
        1 gtatcgattc cgatctgatg aaaatacttc ccgaaaacga tcccgtcata aggcaatatg
       61 agagtttcat agagggaaac tcctcaagag atgtgactta tattgttatc aaaagcgacg
      121 acaagtctcc cgggggtatc gacaacctgg tagccgctgc acgagaaatc tttgacagtg
      181 ccgaccagtt cagcagtata gaggctttcg taaggttcga tgtaatttcg gacctcggac
      241 cgctcggctt tgtgcttata gattcctctg aacttgaatc gttgagcggt tccaatcagg
      301 atatggtcag aaacgtacag agactcgtca attacgactt ttcggccata cacagcatcg
      361 gcgagatgct ggccgggttc gatggattca aagatatgct gaaagaggat gagagcgcgg
      421 ggcgttttga gaatttcatc aggctcccgg aaagtatccc tcagaaagac ccgatgatcc
      481 tggtcatggg tttcaagctc gacggtccct cgtcagatgt ggcgtacgtg gcccgtgtaa
      541 ttccggctct ccggaaatgg ataagcgaaa aactcgaacc gtacgacctt acctacggaa
      601 tggccggcga tcatttcggc acttacgatt cccacagaca ggcaactgac gatttcatgc
      661 taacaaccgt gatctctttg accggtatat cgctgctctt cctgctcgcc tactcgagct
      721 ttaaaatcac tctcttcgtg ttttcatcac tggcggtttc tatgttcata actctgggag
      781 cggcttacct tatttttgga tcgctgaaca taattacctc tttcgtaacc tccataactc
      841 tgggcctcgg tatcgattac ggaattcata tgatcaccag gctttccgac ggcgcaaaga
      901 aagagagaaa cgcgcagaga ctcctcaggg aggcttacaa atctcttttg aagcctttga
      961 tggcaagcat gctaacgacg gccgccgttt tcgtgatact cgcgttgatc gattccccgg
     1021 cagccaggga gcttggaata ctcacttctt tcggcatagt tatatttttc atcgttatga
     1081 ttacgttttt acccgcgctg agtataaaaa ccgtcatgaa acaaggaaga tcggcaaaca
     1141 tccacctttt agatcgattt tttttacggc tggcgaggtt gataaggaaa ctcggtgtgg
     1201 tttccggtgg tatcgtcttc atacttatac tttctctagg ttatctcggt tatataagca
     1261 ttaccaattt ttcctacact ccgccgggat tgatggccac cgattccgaa cagatagccg
     1321 ttccggcgtt gatcgaaaga acgttcggag gaagtatcct gaactcagtt ccattcatgg
     1381 tcggtgacgt tgaatctttg agacgcgcac atgaaaatct aaacaacaat ccgaacataa
     1441 ggagtgtatt ctccgtgctc tcgattctgg aagatggaag tcaggatcag gtgaacagaa
     1501 tacaatcgat cacgagacag ataaattcga tgcgcaactc gccgcttgtg gaggcactgc
     1561 tcaagaaagc acagtactac gatttcatga ttgaacttct cgataaggcc gaaggtgtga
     1621 aaagctccga cgaactcatc gacttaattg cagaggtcat accagacagt ctaaaggaga
     1681 gggttcttta cgaagatggc cagggaaaga gatacttcgt tatcaacgcc gagccgacat
     1741 ctattatcta cagaaacaac gtgatcaaaa tccttttcga cagtgtgggt gaagagctca
     1801 gagaagccgc cggtggttac ccgaaagtct tttattacct gatggatgtt gtacggaaga
     1861 tatcgattcc agtttcactc ttcgcgtttg taatagtttt tttgatagtg accatcgaaa
     1921 ggaagagcct gttcgatggg ttgaaactct tcgccttgat ggttggcatt ctgatgacga
     1981 tgttcggatt gatggaaatt tcaggtatcg agacaacgtt cataacggtc atctcggccc
     2041 cgctcataat aggtattggg gtcgatgcgc tggtgtatgt tgtccacagt tcgggaaaga
     2101 gagacgagaa ggaactcgcc aggacgctga aatccataac gatgtcatct gccacaacga
     2161 tgctggcctt cttttccttt gccttcgcgg aaggtaagct cttgagcagt ttcgggctca
     2221 gtctggcttc gggtgttctt atgtcacttg ttgttgcaac ttttctcgtt cccgtgctac
     2281 catggaacag caaagaacga aatggaggtt aagacatgcc ttttcatctt ttggtcgcgt
     2341 attacgttgt tgatcatgcc tttgtgaaca acaccaaact ttcgaaaatg gacgacaaga
     2401 agctttggac acatttcatc tgggtggttc tggtctttct tgccttcact ttcgacgtgt
     2461 tcctttccaa cccccttggc attctgttgc ttatactctc tatcggcgtg acggtaactc
     2521 tggacatggg aaggaaaaag ctctccaacc atttgataga gataatcgcc ttcttcatct
     2581 ttctgttttt tacgcttctc ggtaagagtt atctgagcag gtcctttgtg accatagaat
     2641 ttcctggtat cttttgggga tgcttgccac aaccgtgggc gtcacgtatt ttctgagagg
     2701 gcggattttg gcggaaaatg cgaccgattc cataggcata gccgaacgta tgtccatatt
     2761 catattcgtt cttgcaggac actgggagtg gactctaata gctatcggcg ccgcgctaac
     2821 cttcagagtg gttttctcga aagatggaaa aatcgaatgg ttaataagtc ccgtagcagg
     2881 tgtcggactg tcattcctct ggcaactgct gatgaggagc atgctgggtt gacctatgct
     2941 gtaggagaca tccacggttg cctcgacgtt ttgagaagac ttgtacagtc tctggaggca
     3001 aagagttccg atgtcttcgt ttttatcggt gactacgttg acagagggcc cgattcgaaa
     3061 ggtgtcatcg actatctgat agccctttcg gaaagatacg aatgtcattt cattagaggc
     3121 aaccatgaac agatgtttct tgattatcta aggcgagaac cgggtgcaat gatctgggca
     3181 tacaacggta tggagagaac cttaatgagt tatggaagtt tgagggccgt tcccgactgt
     3241 cacatcgatt ttctcaacag aactgtctac agctacacga taaagaagtt cgtttttgtt
     3301 catgccggtg tcaggccggg gataccgctc gaagagcaag aagagcgaga cttgctgtgg
     3361 attagagagc cttttctaag caatcgcaac ccgatcaagg gtaagacagt aatcttcgga
     3421 cacacaccac aattcggcgg gccgttgatc gaaaaaagta aaataggaat agacaccggc
     3481 tgtgtctatg gaggctacct tacggcgtac aggatcgaag atagacactg ggtgtgcgag
     3541 aaatgaaaac gggcggaagg tcttccgccc gtttttcttg atggatcgat atatcagggg
     3601 tagatgcctc tcagtttagt cgcgagtgca actttctcta tagcatctac gtaggctgca
     3661 gacctcatat cgatgtcgta cttgttcgcc gttgaggcta cggagttgaa ggcttccttc
     3721 atgatcttgt ggagagccct tctcacatct tcgatatccc agaagtaatg ctgcagaccc
     3781 tggacccatt cgaagtaaga gactgtgact ccaccggcgt ttgcaaggaa atccggaacg
     3841 atgaagatgt tcttggagag tattatttca tccgcttcgg gcgttatcgg gccgttggct
     3901 ccttcgacta ttatcttggc cttgatatcg ccggcattat tcgccgttat ggcgttttcg
     3961 agtgccgcgg ggacgagaat atcgacatcc agagtcaaca gatcgttgtt gctgattttc
     4021 tgaccctttg gataaccttc gatgacaccc ttgttctggt ctctgtaggc tatcacttca
     4081 tcgatatcaa aaccggcagg attgtacaga ccaccggaaa cgtcgctaag ggcaaccact
     4141 ttagagccta tctcttcgct tatcagtttc gccgcgaaag aaccgacgtt tccgaaaccc
     4201 tgaaccgcga aggtggcgtt tttcggatcc atgcccttgt actttgcagc ctcttcggcg
     4261 atgactctga ctcctcttcc agtagcttcc ggtcttccct tcgaaccgcc aatttccagc
     4321 ggtttaccgg tgactatgcc gagaacggag tggccaacgt tcatcgaata agtgtccatg
     4381 taccaggaca ttatctgtgc gttggtgtta acgtccggtg cgggtatatc ctcgttctct
     4441 ccaatgatta tctgtatctc cgaaaagtag cgtctggaaa gcctttccaa ttcacccatc
     4501 gagagttcct gaggatttac gcagactcca cccttgcctc cgccgtaagg aataccgact
     4561 acggcacact tccaggtcat ccaaaaagcc agtgccttta cttcgtccag ggtgacgttt
     4621 tgatggtatc tgattccgcc cttcgccgga cctcttgcaa cgttgtgctg aactctatgg
     4681 ccggtgaata cttttatctc gccgttatcc attctcaccg ggaaattcac cgtgagttcc
     4741 cgcttcgttg aagtgagaac ctgtcgcata ctgctgctca gtttcattac atcagcagct
     4801 ttgttgaact gcttcagtgc attttcaaac aaacttatct cggccacgaa aatacctcct
     4861 gctccttgtt tttggtgtca taccatttga attttatgat tatattcaga gcttcacaaa
     4921 tccatgtacg gtcgaaagga gccgcttaaa gtcaattaaa gactcctagg tgttcttttc
     4981 aaggtgagtt ttctctatgc tagcaactat caaaaaaaag cgaggagaaa acctcgctct
     5041 ggtaccccca gaaggaatcg aaccttcatc tgcggattag gaatccgcca ttctatccgt
     5101 tgaactatag gggcgcaacg tggagattat agcatcacgt tcaagccttt tcaacagctt
     5161 ctctgctaca attggaaagt gctgatctag attgatcgac gggattttgt acagatccag
     5221 agagaggtgg acacatttgg aaagaattgt ggctcttgaa tctacggtta tcgctcacgg
     5281 acttcccttt cccgagaacg tcaacaccgc actcgaaatg gaaagagcgg tgagagagaa
     5341 tggaggtctg ccaaaaacca taggcataat cgatggagat gtaaggatcg gtcttcaaaa
     5401 agaagagata ctttcactcg cgaaagcgag cgacgtgatg aaggtcggga cggccgaggt
     5461 agcctacgcg acggcactca agagaaaggc cgcgacgaca gtcagcgcga ctatgtttct
     5521 ggcaagactt cacggtatct ccgtcttctc caccggaggg ataggtggtg tacacagaga
     5581 tatcgactgg gatgtttcac aggatatcat cgagctgtcc agaaacgata tcatagttgt
     5641 atcggcgggt gtcaagagca tcctcgatgt tcaaaagacc ctggagtttc tagagacgtt
     5701 tcaggtgacg gttctgggct acagaaccga ctattttccg cttttccatt gtagagacag
     5761 tcagtacaag ctcaatctac gggtggactc accagaagaa gtggtggaag ttctgaagga
     5821 gaagaggcgt cttggaataa aaggcggaat tctggtcgcc aatccgatac ccgaagagtc
     5881 ggcaataggt ttggacgaaa tcaaccacct ggtcgatcag gccgtgtcct tggccagaaa
     5941 agagggggta ggcggcaagg ccctgactcc ctaccttttg agcaaaatcg gttttctctc
     6001 ggatggaaaa actctccgtg caaatatatc ccttctgata aacaacgccg ctctcggcgg
     6061 gaaaatagcg gcctctcagg cgtgggtcgt atgaattata tacttaagaa ttcacgggtc
     6121 tattccgttg atactggtga gtttagcgac agggatctgt acataaccga cggtagaatt
     6181 tcagatagag ccggtccgga atccctaacg atggacctgt ccggtaaatt catatatccg
     6241 ggattcgttg attctcacgc tcacctgatc gggacggggc tggagaagat ggtagttgat
     6301 ctgcgaaatt gcagatcgat ggaggatctt cgcgaccggc tgcagataga aagtcatctc
     6361 gtcagggcca gaggctggga tcaggagact ctgggctttt taccggaacg tcggattctg
     6421 gacaggatca cttcgcagcc ggccgttctc gtcagaagat gcggtcatgt ggccactgtc
     6481 aactcggcgg tcatagatcg tttctcgctc gaagcgctgg acggtctgga tgaaaccgat
     6541 atcgagactg gtgtgatcaa ggaaagggca ctcgaaagat tgaactcgat tgtgaagtta
     6601 gcccccgaag aggtcgagga ggccatacgc aggggaagcg tggagtttct gaagtacgga
     6661 gtaacctgtg ttcacagtga tgatcttcat ggtatggaac ttgagacgct ggttgagagt
     6721 ttgaagcgaa tagacaacat aagagtattc gaaaagatag ccgtggaaag acctgatcag
     6781 ctttcgatgg tcagtatggc cagaaacgga gaaacgaggt ttttcaaagt tatcgccgcg
     6841 aagatatatc tggacggctc tctcggagca aggacagcgg ctatgaaaga accgtattcc
     6901 gattcgcccc gtgaaactgg tgtcctatac atggattgcg aaactctgaa aagtatcgtc
     6961 gaaagggccg accgtgaagg aattcaacta tgtatccatg ttatcggcga tagatcgctg
     7021 gaagaagcgt tgaaagcctt tcacggttct agagcccttg aattgaagca ccgactgata
     7081 cacgtacaga tagcatctcc ggaagagatc cacagaatcg ccgacctggg attgacggct
     7141 tcgattcagc cggttttctt cgattccgac gcacatatcg ctcccggccg ttttggagag
     7201 gcacggataa aaaatgctta ccctttcaga caaatgaggg acgcgggaat cagaatgg
//
LOCUS       DAOOLY010000111         7226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12235_length_7226_cov_4.166504, whole genome shotgun sequence.
ACCESSION   DAOOLY010000111 DAOOLY010000000
VERSION     DAOOLY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12235_length_7226_cov_4.166504"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1788)
                     /gene="aspS"
                     /locus_tag="PLD15_06790"
     CDS             complement(16..1788)
                     /gene="aspS"
                     /locus_tag="PLD15_06790"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPB63646.1"
                     /translation="MKKRTHTCGELRISNTGTVVTLNGWVDRVRDLGGIKFVLLRDRY
                     GFTQVVFDPRNEALYSIALKLGNEYCVSVTGTVRERPGDARNPGMSTGDIEIVADELE
                     ILSESAIPPIYINIDEESSEEMHLRYRYLDLRRSAMQNNIITRHRFVQAVRSYLNGNG
                     FIDVETPALGKSTPEGARDFLVPSRLKPGKFYALPQSPQIFKQLLMVAGLDKYYQIAK
                     CFRDEDFRADRQPEFTQIDIEMSFATEEDVFSTTEGLMKHAFKEAVGVDLETPFKRFT
                     YREVMDLYGSDKPDTRYAMEIRDLSPQFDGTSFSLISDALGHGMKVKGFMARGLAGKF
                     SRKRIDDFTQQSKELGLGGLMWIKVESDSIKSSIIKHCQREVEKVVEFFGARVGDLIL
                     LSIGESLPLSRALGQLRIRIVKEENLKAEGFDFLWVTEFPMFSFNKEEGRIEAEHHPF
                     TMPDLDDLEEFADTQPLKVRSRAYDLVLNGYEIASGSVRIHRQDIQSRVFDLIGLSRE
                     EAMEKFGYLLEAFQYGPPPHAGIAPGLDRIVAIIVGAPSIRDVIAFPKVASGADLMTS
                     APSEVSHKQLDLLKLQLKDSGDRE"
     gene            1895..3637
                     /locus_tag="PLD15_06795"
     CDS             1895..3637
                     /locus_tag="PLD15_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPB63647.1"
                     /translation="MKKSLLLASLLIFGLVVSSVAAPVNYVDVAQNHWAYNAVINLTN
                     LGILNGVQGADGKLYFNGNDPLTRYQTAVMLQRTIDYVELNFARQGTIPQSSVSDAGL
                     RSRLEALELSLTDASGKLIDTLDLQLRLTALENRVNSVGVGTTGTTVTQSTIDSLRNQ
                     IMKFVEDLSLTTKKIDTLALDVQNMQNSMANLSVMDAKVNDAVRRVDTLSSSLSAIQM
                     SFSTHDRAISNLETSVQTISTEVNEFDAKISTLTNRASTNAMEIASIKDTINTMSGNT
                     SSIRDLQNAINSLETQIANIKLPADATKKLDDLSARVNTIASEYVKVGDTQNFVTKTD
                     LKANLNLYAGIDELAKVKEDSASLANSLAALRGDFNSETGIIKSDIGNLQRSLNAVNE
                     IVAVHENDLSSLKNSLSLVTSLRTDLTALNSKFNALTDQQAMNLSATQANIADLEKRM
                     NEAHNLLKVSIDASLSKIALSMDTVNTQLGKNENDIKTLKTRTDTLESRLNATVTNLD
                     RYLKTSDLETQPVITNLSDRVAEINKTTVDAASKSDVETLQKKLQPWLIFSVISGLAG
                     LGVAIYVLIAGPIS"
     gene            3724..5022
                     /gene="asnS"
                     /locus_tag="PLD15_06800"
     CDS             3724..5022
                     /gene="asnS"
                     /locus_tag="PLD15_06800"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPB63648.1"
                     /translation="MKVVDISDLRSHTGEEVTVRGWLRRKRSSGKIQFLFLRDGSGFV
                     QAIAERSSVSDGVFQNANNLKMESSVVVKGVVKAEERAPGGVELLVTDIEVVQIPEKD
                     FPINKPDHSIDFLMDNRHLWLRTQRQTHILKIRHEIIKAVREFYNDRDFVLVDTPIFT
                     GSIGESAGNTFEIDYFDYGKAYLAQTGQLYLEAAAMALGKVYNLGPTFRAEKSKTRRH
                     LIEFWMNEAEVAYYKHEDNIKLQEDLVSYVVQKTLERAGDNLLAIGRDIGKLKKIEAP
                     FPRMTYDEAVRLLQKKGFTIAWGDDIGADEETAVASEFDKPVVVEKYPKMMKAFYMQP
                     DPERPEVVFCDDMLAPEGYGEIIGASERIHERELLIERIKEYGLDVSSYDWYLELRDY
                     GTVPHSGFGMGIERVVAWIAGLEHIREAIPFARTLYRIHP"
     gene            5032..6078
                     /gene="ruvB"
                     /locus_tag="PLD15_06805"
     CDS             5032..6078
                     /gene="ruvB"
                     /locus_tag="PLD15_06805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPB63649.1"
                     /translation="MGEERFLTPGEVQEDSTGKSLRPKTLSEYIGQPHIRKRLGISIE
                     AAKVRGEALDHVLLAGPPGLGKTTMAHIISNELGANIYVTSGPVIEKQGDLAAILTGL
                     DRNDVIFIDEIHRLNRSVEEILYSAMEDYQLDIMIGKGPSARSIRLDLNPFTLVGATT
                     RSGFIGAPLRNRFGMIMEMEFYSEMDLKQIIQRSAGLLDTSITEEAALLLARRSRGTP
                     RIANRLLRRVRDVVTIEGGREITIDTVQSAMTLMSIDEDGLDAMDKKILTVLIENYHG
                     GPAGLKAIAASVGIEPETISEVYEPFLLQTGFLVRTNRGRMVTDKAYRHLGRTEEGGS
                     GLLWNFDTGNQDEV"
     gene            6080..6283
                     /locus_tag="PLD15_06810"
     CDS             6080..6283
                     /locus_tag="PLD15_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63650.1"
                     /translation="MFLLSNFLYSIAVVARLFLQFEVLSIIVFTLLGLFNPYMIPGVK
                     SFFWKDVQTHYGPGAKVVPFLER"
     gene            6491..7207
                     /locus_tag="PLD15_06815"
     CDS             6491..7207
                     /locus_tag="PLD15_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPB63651.1"
                     /translation="MDGSSCQVEDATVIITFGGDGTVLKAVPTAYTRNLPIMSFRVGS
                     VGFLASFEINMLHAILDLFLQNALQSSERTLMKITFKDEVYYSLNDFVIERSSPSRTV
                     TFTVSIDGQSSYPIVGDGIIFSTNTGSTAYSMAAGGALVDPEALCFQITPICPHNPFV
                     GSLVLSSFRKIRLEVTQDKGFPLEAYVDGIFSDRLSKGDVVEVQMSKKKIRLLREGNL
                     DFVALLKKKLAFGGRLKDDI"
BASE COUNT         1898 a         1730 c         1838 g         1760 t
ORIGIN      
        1 cgtcgatagc gattctcact cccgatcgcc cgaatctttc agctgaagtt tcagcagatc
       61 caattgcttg tgggagactt ccgagggggc cgaagtcata agatcggctc cactggcaac
      121 ctttggaaag gctattacat ctctaatcga tggagccccg actatgatcg cgactattct
      181 gtccagccca ggggcgattc cggcatgtgg tggaggaccg tactggaagg cctccagaag
      241 gtagccgaac ttctccattg cctcttctct ggagagacct atgaggtcga aaactctgct
      301 ttgtatatcc tgacggtgta ttctgaccga accgctggcg atttcgtaac cgtttagaac
      361 cagatcgtaa gctctggatc tgactttcag aggctgtgta tctgcaaatt cctcaagatc
      421 gtcgagatcc ggcatggtga agggatggtg ctcggcttca atccttccct cttctttatt
      481 gaaagagaac atcgggaatt ccgtcaccca aaggaaatcg aaaccttcgg ccttgaggtt
      541 ttcctctttc actatcctta ttcttaattg accgagagcc ctggaaagcg gaagactttc
      601 acctatgctg aggagtatca aatcgcccac cctggcgccg aagaattcga ctaccttctc
      661 aacttctctc tgacagtgtt tgattatgga ggatttaatg gagtcgcttt cgactttgat
      721 ccacatcaaa ccgccgagcc ccagctcttt ggattgttgt gtgaaatcgt cgattctctt
      781 tctggagaac ttccctgcca gtcctctggc catgaaacct ttgaccttca ttccatgacc
      841 cagcgcgtcg cttatgagtg aaaaggaagt gccgtcgaac tgtggtgaga gatctcttat
      901 ctccattgcg tatctcgtgt cgggtttatc gctcccatac agatccatta cttctctgta
      961 ggtaaatctc ttgaaaggtg tctcaagatc tacgccaacg gcctccttga aagcgtgctt
     1021 catcaatcct tccgtcgtcg agaagacatc ctcctcggtc gcgaaggaca tctctatgtc
     1081 tatctgggtg aattcaggct gcctatcggc cctgaaatcc tcgtccctga aacacttggc
     1141 tatctggtag tatttatcga gtcccgccac catgagcagt tgtttgaaga tctgcgggga
     1201 ttggggaagc gcgtagaatt tgccaggctt caatctcgaa ggaacgagaa agtctctcgc
     1261 gccttcgggt gtggattttc ccagcgcggg cgtctcgaca tctataaaac cattcccgtt
     1321 aaggtatgat ctgacggcct gaacgaacct gtgcctggtt attatgttgt tttgcatagc
     1381 agagcgtctg agatcgagat accggtacct caggtgcatc tcttcggaag attcttcgtc
     1441 tatatttata tatattggtg gtatagccga ttcagagaga atttccagtt cgtcggcgac
     1501 gatctctata tctccggttg acattcccgg atttcgcgca tcgccgggac gttctctgac
     1561 ggtcccggtt acagaaacac agtactcgtt gccaagtttc agtgctattg aatatagagc
     1621 ttcgttccgc ggatcgaaaa ccacctgggt gaaaccgtat ctatcacgta aaagaacgaa
     1681 ttttattcct ccgagatctc ttactcgatc cacccagccg tttaacgtta ctaccgttcc
     1741 agtgttcgat atcctcaatt cgccgcaggt atgagtcctc ttcttcaagt acaacacctc
     1801 ccccattcat aaatattacc acgggagttt ataggatgga ataatgattt gtggtagaat
     1861 caattcgaat atttccttcg gggaggtgta gaaattgaaa aagagtcttt tgcttgcctc
     1921 actgcttatt ttcggtctgg tagtctcttc agtggcggcg cccgtcaatt atgtcgatgt
     1981 tgctcagaat cactgggctt acaatgcggt tatcaacctg acgaacctcg gtatattgaa
     2041 cggtgttcag ggtgccgacg gcaaactgta cttcaacggt aatgacccgc tgaccagata
     2101 tcagacagca gtaatgctac aaagaaccat agactatgtt gagttgaact tcgccagaca
     2161 gggtacgatt ccgcagtcaa gtgtatcgga cgccggtcta agatcgcggc tggaagctct
     2221 cgaactgtct ttgacggatg ccagcggaaa gttgatcgat acgctcgatt tgcagctgag
     2281 attgacagcc ctcgaaaaca gggtgaactc cgtaggcgtc ggaacgaccg gaaccacagt
     2341 gacacagagc acgatcgatt cgctcagaaa ccagataatg aagttcgtgg aggatctgtc
     2401 gctgactaca aagaagatcg acacgctcgc cttagacgtt cagaacatgc agaacagcat
     2461 ggccaacctt tcggttatgg atgctaaggt caacgacgcc gtcagaaggg ttgatactct
     2521 gagcagtagt ttgagcgcca ttcaaatgag tttctcaacg cacgacagag ccatatcgaa
     2581 cctggaaacc agcgttcaga ctatctcgac tgaagtgaac gaattcgatg ccaagataag
     2641 cactctgacg aacagagcct ctacaaacgc catggagata gcatctatca aagataccat
     2701 caacaccatg tctgggaaca cgtcttccat cagggatctt caaaacgcta ttaattctct
     2761 ggaaacacag atagcgaaca tcaagctccc tgccgacgca accaagaaac tcgacgatct
     2821 atctgcaagg gtcaatacga tcgcatcgga atacgtcaaa gtcggtgata ctcagaactt
     2881 cgtcaccaag accgatctga aggccaacct gaacctctac gccggaatcg acgaactggc
     2941 gaaggtaaag gaagacagcg cgtcgctggc caacagtctt gcggctttga gaggcgattt
     3001 caattcggaa acgggtatca tcaagagcga tatcggcaat ttgcagagaa gcttgaacgc
     3061 agtcaacgag atagtggccg tacatgaaaa cgacctctct tcgctgaaga attcgttgag
     3121 tcttgtaacc tctctcagaa cagatctcac ggcattgaac agcaagttca acgccctcac
     3181 cgatcagcag gctatgaatc tttcagccac tcaagcgaat attgccgatc ttgagaagag
     3241 gatgaacgaa gcccataacc tgctcaaagt ttcgattgat gccagccttt cgaagatcgc
     3301 gctgagcatg gatactgtca acactcaact cggtaagaac gagaacgata tcaagacgct
     3361 gaaaaccagg accgatacgc tcgagtcaag gttgaacgca accgtcacta atctcgacag
     3421 atatctcaaa accagcgacc tcgaaaccca gcccgtcata accaacctct cggacagggt
     3481 cgcggaaata aacaagacaa cggtcgacgc cgccagcaag tccgacgtgg agactctgca
     3541 gaagaagctg caaccctggt tgatcttctc ggttatcagt ggactcgcgg gacttggagt
     3601 ggccatctac gtgttgatcg ccggaccgat atcttgatgt catagttact gataaaaaac
     3661 ccggggcctg acccgggttt tttgttataa ttgaagctga aaagttcccc ctggaggtgt
     3721 tccatgaaag tcgtagatat ttcggacttg agaagtcata ctggagaaga agtcacggta
     3781 cggggctggt tgagacgaaa gagatccagt ggaaagatcc agtttctctt tttgagggac
     3841 ggtagcggtt ttgtacaggc gatcgcggag agatcatcgg tttccgacgg cgtttttcag
     3901 aacgccaaca acttgaaaat ggagtccagc gtcgtagtga agggtgtcgt taaggctgaa
     3961 gaaagggcgc cgggaggggt tgagctcctc gttacggaca tagaggtggt acagataccc
     4021 gaaaaagact tcccgatcaa taaaccggat cactcgatag attttctgat ggacaacagg
     4081 catctgtggc tcagaactca gagacagaca catatactga agatcagaca cgagataata
     4141 aaggccgtca gggagttcta caacgaccgc gatttcgtcc tggtggatac accgatcttc
     4201 accggatcga tcggcgaaag cgccggcaat accttcgaga tagattactt cgattacggc
     4261 aaagcgtacc tggcccagac cggtcagctc tatctcgagg cggcggcgat ggcgctggga
     4321 aaggtttaca acctgggtcc gactttcagg gccgaaaagt ctaaaacgcg acgtcacctc
     4381 atagagttct ggatgaacga ggccgaggtt gcctactaca aacacgaaga taacataaag
     4441 ttacaggaag atcttgtctc atacgttgtg cagaagaccc ttgaaagggc cggcgacaat
     4501 cttctggcga tcggaagaga tatcggcaaa ctcaaaaaga tagaagcccc cttcccgaga
     4561 atgacttacg acgaggccgt caggcttctt cagaagaagg gtttcacgat agcctgggga
     4621 gatgacatag gcgccgacga agagacggcc gtcgcgagcg agttcgataa gcccgtcgtt
     4681 gtcgagaagt atccaaaaat gatgaaggct ttctatatgc aaccagatcc cgaaagaccg
     4741 gaagtcgttt tctgcgatga tatgctggct ccggagggtt acggtgagat aataggcgct
     4801 tccgagagga tacacgagcg tgagcttctg atcgagagaa taaaggagta cggtctggac
     4861 gtctcatcat acgattggta tctggagctt agagactacg gaacggttcc ccacagcggt
     4921 ttcggcatgg gaatcgaacg tgttgtagcc tggatcgcag gtctggaaca tataagagag
     4981 gctataccct ttgcaaggac tctgtacagg attcatccct aggaggtctc catgggggaa
     5041 gagaggtttt taacacccgg tgaggtacag gaagacagta ccggcaaaag tctgcgcccg
     5101 aaaactctca gcgagtatat cggtcaacct cacataagga aaaggctggg catctccata
     5161 gaagcggcca aagtcagagg agaagctctg gatcacgtat tgcttgccgg gccgccaggt
     5221 ctgggaaaga ccaccatggc tcatataata tctaacgaac taggagccaa catttacgtc
     5281 acatcgggac cggtgataga aaaacaggga gatcttgcgg ctatcctcac aggtctggat
     5341 aggaacgatg taatcttcat agatgagatc cacaggttga accgcagcgt cgaagagata
     5401 ctctattctg cgatggagga ctaccagctg gatataatga tcggtaaagg cccaagcgct
     5461 cgctcgataa gactggatct aaaccccttc actctcgtag gtgcaaccac cagatcgggt
     5521 ttcattggtg cgcctttgag aaacaggttt ggcatgatca tggagatgga gttctattcg
     5581 gaaatggatc tcaagcagat aatccagcgt tccgccggtc ttctggatac atcgataacc
     5641 gaagaagccg cactgctcct ggcgcgacgg tcgagaggaa ctccgagaat cgccaatcgc
     5701 ctcctaagaa gggttagaga tgtagtgacc attgagggag gccgcgaaat aacgatagac
     5761 actgtacaat cggcgatgac gttgatgtct atcgacgaag acggacttga tgccatggac
     5821 aaaaagatac tgacggtgct cattgagaac taccacggcg gacctgccgg tttgaaagcg
     5881 atcgccgctt cggtaggtat cgagccggaa acgataagcg aagtttacga gccgtttctg
     5941 ctgcaaaccg gttttctggt aagaacgaat agaggtagaa tggttaccga caaggcctac
     6001 agacatctcg gcagaaccga agagggtggc tccggtttac tctggaattt cgatactgga
     6061 aatcaagacg aggtttgaag tgtttctcct ttcgaatttt ctgtattcga tcgccgttgt
     6121 ggccaggtta tttttgcaat tcgaagtgct gtcgataata gtattcactc tgttagggct
     6181 tttcaatccg tacatgatcc ccggtgtaaa gagttttttt tggaaagatg tccagactca
     6241 ctatgggccc ggtgcaaagg ttgtgccctt tcttgaaagg tgatgcactt gatttcacgc
     6301 cagtcgtgac ggtgctggca atagttttca tagatttttt cctggtttct tctctttttg
     6361 atctggcgca aaatctgagg tgagtcaatt gaaggccgct gttttttgta acagaaccag
     6421 aattgcgtcc gctacattag accttttgac acgggagata gagtcccacg gccttggggt
     6481 ttatcattgc atggatggca gttcctgcca ggttgaggac gccaccgtca taatcacttt
     6541 cggtggagac ggtaccgttc ttaaggctgt tccaacggcc tataccagaa atttacccat
     6601 catgagcttc agggttggca gtgtgggatt cctagcttcc ttcgaaatca acatgcttca
     6661 tgcgattctg gacctctttc tccagaacgc actgcaatca agtgagagga cattgatgaa
     6721 aattaccttt aaagacgagg tctattactc gttgaatgat tttgtcatag aacgatcttc
     6781 tcccagcagg acagtaacat ttaccgtatc gatagacgga cagagtagct atcccatcgt
     6841 tggagacggg ataattttct cgaccaacac cggttcgact gcgtattcga tggctgccgg
     6901 aggcgcgctg gtggatcccg aggccctttg tttccagata acaccgatct gtccacataa
     6961 ccccttcgtg ggctctctcg ttctctcctc cttcaggaaa atcaggctcg aagttacgca
     7021 ggataaggga tttccactgg aagcctacgt agatggcatt ttcagcgatc ggctgtctaa
     7081 aggcgacgtg gtggaagtcc agatgtccaa aaagaagatc aggctactcc gtgaagggaa
     7141 cctggatttc gtggcgctgc tgaaaaagaa actggctttc gggggaaggt tgaaagatga
     7201 tatctaaggt cggacttgaa agaata
//
LOCUS       DAOOLY010000112         7210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12290_length_7210_cov_4.608106, whole genome shotgun sequence.
ACCESSION   DAOOLY010000112 DAOOLY010000000
VERSION     DAOOLY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_7210_cov_4.608106"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLD15_06820"
     CDS             <1..259
                     /locus_tag="PLD15_06820"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB63652.1"
                     /translation="ASLFLTGKDPAVIDEVYDFCETVGLPTTLAEIGLGNVTDEQLMK
                     VASASCAEGETIHNELVEITPQTVFSAIKAADAYGKYRKQE"
     gene            361..1287
                     /locus_tag="PLD15_06825"
     CDS             361..1287
                     /locus_tag="PLD15_06825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63653.1"
                     /translation="MGFKIRTYEREEDYWKLREFLREVFKANGLREYSWHLARLDYWR
                     WHIMENCLKIDSLNDCIFFWEDSNDRIVAAVNRDGTGEAHLQVHPSFDCEELEEEMLE
                     VSERNLFSEKDGLRSLWVFTDSQDKRRIDILKRRGYSQGDWPEHQHRRDLSGQIAEVY
                     IPEGFDIRSLGTAEELPSRSWASWRAFHPNEPDEKYEGWEWYLNIQKQPMYRRDLDIV
                     AVAPDGEIAGFCTIWYDDVTRTACYEPVGVMPEYHKRGLGKAMLTEGLHRLKRMGAVR
                     VFVGGYSTAANALYDSAVSPVCDLNEPWLKKF"
     gene            1476..2330
                     /locus_tag="PLD15_06830"
     CDS             1476..2330
                     /locus_tag="PLD15_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPB63654.1"
                     /translation="MKKTRLVLIVLTFICLHLSAAQPNESSFVRVSGGSFLMGSVSEK
                     SFDNESPVHVVDITYDFLISKYETTFAQFDEFCEQTVREKSSDNGWGRQNRPVIFVSW
                     WDATEYCNWLSSKAGFPPAYNSSGYLIDGKGEVTSSPSKAVGYRLPTEAEREYVARGA
                     DLSKTTLYSGHDEPDIVAWYNINSDWKTSEVGAKQPNEIGVFDMSGNVWEWCHDWYAS
                     YPYLEQTDPCVFSEGNYRVIRGGSWHDFEKYLRVSARSGYTPTGAAKDVGFRVCRTLS
                     RHTLEPDY"
     gene            complement(2400..3014)
                     /locus_tag="PLD15_06835"
     CDS             complement(2400..3014)
                     /locus_tag="PLD15_06835"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPB63655.1"
                     /translation="MKRIILSSIILVILVLSATTALSSGPIQIKPPILATAVGDGEGS
                     VILNLVCGWKGIECESVLDLTPARLEERLSGIDGPGTLVVATGAIVEGDLYTICNIEK
                     MSIEKESSRIEGLIAVAKSSRIPVIGLHIDRGITSPDTDPGKSLDLIMPYADAIILVT
                     HIGMDEYFEEIAAAGAIPLIVIDNSDKLVEALEALFSMNLGECE"
     gene            complement(3219..4496)
                     /locus_tag="PLD15_06840"
     CDS             complement(3219..4496)
                     /locus_tag="PLD15_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63656.1"
                     /translation="MRKSFTGREASMKEEKVKVGIGFATGRRYFQRVLRSYVYNWKES
                     RLVDSEKFSLNLFVAYDLSYRNTKKSHYTQLSSSVDEMLDSKCFIGQEEVEEEKKSLV
                     KEGVVSSEEVSLVFGKGYASQRNIVLYSALKNHVDYLLYLDDDEYPVAVTDKLGSTSW
                     IGQHVLKTHLKNIDRADITYGHHCGYISPIPSIEFSTTLPEDTFRLFIGAISNDIVKW
                     ETLKKLIKAGGVSYADPEILNSDDVVEVPEIKHAKFISGSNLCLNLKDPGRLFPFYNP
                     PDARGEDTFLSTCLSERKVLRVPCYAFHNAFSTYNHILMGVLPTKLRPIVADSKEIVR
                     RFYRSCIGWIRYKPLLLYITDRDRYSETIEEMKTSLKRTLPAISAHFGTESFFNISKE
                     LNKYHRLVEKHYDQFARVKRIWSNICSYLREID"
     gene            4734..5555
                     /locus_tag="PLD15_06845"
     CDS             4734..5555
                     /locus_tag="PLD15_06845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB63657.1"
                     /translation="MGIYDFLLSGRDQWEMVGKGFMNGQEEIEKRFFETPEEQLSPAD
                     QLIKAVALWYRGKFDEFKRLMKRSREAIYTEEYNSAMVQSVYHTVELIKNENGVVLDL
                     ATGMGGLLEELLTYTEREYISVDISPSSSFGLLQYLRYRGWGDRVYQIIADGKKLPFR
                     NDSLDLIVSAAGFQNMENSREVFTETRRVSRKLITLCIFFEEDDPNLSHVKDRNLHVS
                     RLFIEGLEEAGWNVTVENVIKARAEPTPQSMILGIRPDQLPVTPTTRNFEIIVAE"
     gene            5660..6874
                     /locus_tag="PLD15_06850"
     CDS             5660..6874
                     /locus_tag="PLD15_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPB63658.1"
                     /translation="MKRGHHSKNHGQEWSVAAALLNGCNTIEQLKDYFSIMGRRFGIF
                     FEIFPYGKKAHEEKDLDSFLNTAIQKMKESGWVIQDGERYLLTGKGRHQAKEMLAELE
                     NSGRLLARATQPKTVAKITVIAHFVLAALKLPAALLSGSTGLLNDSFDTLLDGVSSIF
                     VYWGVKKKRENLVSFILLFFMGITGIFSLFEALSRLVSGEIPSPDLLTFAAVVISGLI
                     CAFLWFYQKYSGLKNRSFPLITQSTDSRNHVLVAVSVSIGLIVSLMKVPYVDALVGIM
                     ISLLILKGATDLLIDLIKSSRGEAMDFEKYGFSLFNKFRAKQLKRWFLFMIEQGKIKT
                     REQLICEAKASMVYQDIEPLRTLGLSDSQFDELIVNTALEALDSEKLISETAGQLSLT
                     EEGFSELKFTRK"
BASE COUNT         1942 a         1608 c         1776 g         1884 t
ORIGIN      
        1 cgcttcgctt ttcctgacgg ggaaggatcc ggcagttatc gatgaagttt acgacttctg
       61 tgaaacggtc ggacttccca cgactctggc ggaaatcggt ctgggcaacg ttaccgatga
      121 gcagctgatg aaagtcgcaa gcgcttcctg tgcggagggc gaaacgattc acaacgaact
      181 ggttgaaatc accccccaaa ctgtgttctc cgccataaag gcggcggacg cgtacggaaa
      241 ataccgcaag caagaataga atatttttca acaagccaac catgccgggc gtgaaaaatt
      301 tcgcccggct ttatcataga ataggacaat ccagtattga ttagaagtgc gggtgatgag
      361 atgggattca aaatacgaac atatgagcgc gaagaagatt actggaaact gagagagttc
      421 ttgagagaag tattcaaagc caacggactg cgcgaatact cctggcatct ggccagactg
      481 gattactggc gctggcatat catggagaac tgcctgaaaa tcgactcgtt gaatgactgt
      541 attttcttct gggaagattc taatgacagg atagtggcgg ccgtcaaccg tgatggaact
      601 ggcgaggccc atctacaggt acatccatct ttcgactgtg aggagctgga ggaagagatg
      661 ctcgaagtct cggagaggaa ccttttcagt gagaaagacg gcctcagaag cctctgggtg
      721 ttcaccgact cccaggacaa acgaaggata gatattttga agagacgagg ttactcgcaa
      781 ggtgactggc cggaacatca gcatcgacga gacctgagcg gtcaaattgc agaagtctac
      841 attccggaag gcttcgatat acgctccctt ggaactgcag aagaacttcc ttccagaagc
      901 tgggcttcat ggagggcctt tcatccgaat gagccggacg aaaaatacga gggctgggaa
      961 tggtatctca acatccagaa gcagcctatg tacaggcgag atctagacat tgtcgcagtt
     1021 gctcccgacg gcgagatagc aggcttctgc acgatatggt acgacgacgt caccaggacc
     1081 gcatgttacg agcctgtcgg ggttatgccg gagtaccaca agagaggtct ggggaaggcc
     1141 atgctaaccg aagggctcca cagactgaag agaatgggag cggtaagggt cttcgtgggc
     1201 ggctactcca cggccgcaaa tgctctgtat gactcggcag tctctccagt ctgcgacctc
     1261 aacgaaccct ggctgaagaa attctgattt tcgatccctt ataccgacgg cccggatgaa
     1321 tgtttactgg cggtttcttg tatacagttc gggtcaaaga aacgaggagc gttgggatag
     1381 agctcgttgc cagtcttatt gggggccggc cttctttcac aggcaagctc ttacgttatt
     1441 tacccttttg agagtgtatc caatggaggt cctatatgaa aaaaaccagg ctggtattga
     1501 tcgtgttaac cttcatttgt cttcatcttt ccgcggcaca gccgaatgaa tcctcttttg
     1561 ttcgagtgag tggaggaagt tttttgatgg gcagtgtttc agaaaagagt ttcgataacg
     1621 agtcgcctgt tcacgttgtc gatatcacat acgatttttt gatttcaaag tacgagacga
     1681 ctttcgcgca attcgatgaa ttctgcgaac aaaccgtacg ggagaaatct tctgacaatg
     1741 ggtggggaag gcaaaacagg cccgtgatat tcgtgagctg gtgggacgca acagagtact
     1801 gcaactggtt aagctcaaag gcagggtttc ctccggctta taattcttct ggatatctta
     1861 tagacggaaa gggagaagta acttcgtcac cttcaaaggc ggtggggtat cgtctgccaa
     1921 ccgaagcaga gcgggagtat gtcgcaagag gggcggattt aagtaaaacc accctctact
     1981 ctggacatga tgaaccggac atcgttgcgt ggtacaacat caattccgac tggaaaactt
     2041 ctgaagtggg agcgaagcaa ccgaatgaaa tcggggtttt cgacatgagt ggaaacgtct
     2101 gggaatggtg tcatgactgg tatgcaagtt acccgtatct tgagcagacc gacccctgcg
     2161 tcttttcaga gggaaattac agggttatca ggggtgggtc ctggcacgat ttcgaaaaat
     2221 acttgagagt gtcggcaaga agcggctaca caccgacggg agcggcaaaa gatgtcggat
     2281 tcagagtctg tagaacgctt tctcggcaca ctctagaacc ggactattga aactatctgg
     2341 attttagtga agcaacgaca cgatacagca acccagaagc gaaattcaac cgacgtgctt
     2401 tattcgcact cacccagatt catcgagaag agcgcctcga gagcttcgac gagtttgtct
     2461 gagttgtcga tcacgatcaa cggtattgcg ccggccgcag cgatctcttc aaaatactcg
     2521 tccatgccga tgtgcgtgac aagaattatg gcatcagcgt aaggcattat aagatctaaa
     2581 gacttgccgg gatcggtatc gggagaggtt attccccggt cgatgtgaag accgatcacc
     2641 ggaatcctgc tggattttgc gacggcgatc aacccttcta ttctcgacga ctccttttct
     2701 atgctcattt tctcgatatt gcaaatcgta tagagatcgc cctccacaat ggccccggtg
     2761 gcgacaacaa gcgtccccgg gccgtcgatt ccactcagtc tctcctccag ccgggcagga
     2821 gtcagatcga gaaccgactc gcattctatt cccttccatc cgcaaacgag gttcaagata
     2881 accgagccct ctccatcgcc cacggccgtt gccagaatcg gtggcttgat ctgtataggc
     2941 ccgcttgaaa gagcggttgt tgcgcttagg accagtataa ccagtatgat agagctcaaa
     3001 attattcgct tcattttttc tcctccaaat aatagttgtt ccatatatta atatgactaa
     3061 ttttatcaga tttattcact ctgtgcctgt agaatttatt atccaggtag aaactttgaa
     3121 aactcccgcg ctttcgttat aggttctata agtattgacg ggctgagaac gttttcttca
     3181 ggtagcagaa gaagacctca tctgcattgg ttgtagattc agtcgatctc tcgcagataa
     3241 ctgcatatat tcgaccatat tctctttact ctcgcgaatt gatcgtagtg tttctcaacc
     3301 agcctatggt atttgttcaa ctctttggag atattgaaaa aactctcggt gccaaagtgc
     3361 gccgagatcg cgggcagtgt tctcttcaga ctggttttca tctcttcgat cgtttcggaa
     3421 tatctatcac ggtcggttat atagagaagg agcggtttgt atctgatcca tccaatgcag
     3481 gacctgtaga aacgccttac tatctctttt gaatcggcta ctatcggtct gagctttgtg
     3541 ggaagtacac ccatcaaaat gtggttgtaa gtcgagaagg cgttgtgaaa agcgtaacag
     3601 ggaactcgaa gaacttttct ctcactcagg caggtgctca aaaatgtatc ttccccgcgt
     3661 gcgtcggggg ggttataaaa ggggaatagc ctgcccgggt ccttcagatt caggcataga
     3721 ttcgatccag atatgaactt ggcgtgtttg atttccggaa cttccacaac gtcatcgctg
     3781 ttcaatatct cagggtcggc atacgatact cctccggctt tgatcaattt cttaagtgtc
     3841 tcccacttga ctatgtcgtt gcttatcgcc ccaatgaaca gtctaaaagt atcctcggga
     3901 agagttgtcg aaaactctat ggaagggatc ggcgatatat agccacagtg atggccgtaa
     3961 gtgatatctg cccggtcaat gtttttcaaa tgggttttca atacgtgctg gcctatccac
     4021 gaagttgagc ctagtttatc tgttacggca actggatact cgtcatcatc cagatatagc
     4081 agatagtcaa cgtgattctt aagtgcacta tagagaacga tgtttcgctg agaagcatat
     4141 ccttttccaa acaccagaga aacttcttca gaactgacga ctccctcttt tacaagagac
     4201 tttttctctt cttcaacttc ctcctggcct atgaagcatt tcgaatcaag catctcatct
     4261 acactagaac tcagttgagt ataatgactt ttcttcgtat ttctataact cagatcataa
     4321 gctacaaaga gattcagact gaatttttcg ctgtcaacca atctcgattc cttccagttg
     4381 tacacgtaac tcctcagaac tctctggaag taccttctcc ctgttgcaaa accgattcca
     4441 acctttactt tttcctcttt catggaagcc tcccttcctg tgaacgactt tctcatacag
     4501 ttactttcac gcttcgaccg tccaatttta tcacgggcta attttccaac ggggcgaatc
     4561 ccatttttga atcgtcggga aagatagtaa aatacttact ggatgtatat aaggagggag
     4621 acaaaatgat tgaagtagag agtttgttgc agtgtccttc gtgtagctct tctcttttaa
     4681 gccaagaaga tagtttcatg tgctcccgtt gcggtaagag attcaaaaag cgaatgggaa
     4741 tctatgattt cttgttgtcc ggaagggacc agtgggagat ggtaggaaag ggcttcatga
     4801 acggtcagga agagattgaa aaacggttct tcgagacccc tgaagagcag ctttctcccg
     4861 ccgatcaact aataaaggcc gttgccctgt ggtaccgcgg aaagttcgat gaattcaaga
     4921 ggcttatgaa acgttcaaga gaggccatat acacagagga atacaacagc gcgatggttc
     4981 agagcgtcta ccataccgtc gaactcataa agaatgaaaa tggtgttgta ctggatctcg
     5041 ctacaggtat gggcggtctt cttgaagaac tcctcacgta tacggagcga gagtacatat
     5101 cagtagacat aagcccatcg agcagttttg gactccttca gtatttgaga tacagaggtt
     5161 ggggagacag ggtttatcag attatcgccg atggtaaaaa actgccgttc agaaacgact
     5221 ctctggactt aattgtaagt gcggcgggat ttcagaacat ggagaactcc agggaagtct
     5281 ttacggagac tcgaagagtt tcaaggaagc tgattactct gtgcatcttt ttcgaggaag
     5341 acgatccgaa tctatcacat gttaaggaca ggaatctcca cgtttcgcgg ctttttattg
     5401 aaggccttga agaagctggc tggaatgtaa ccgttgagaa tgtcattaaa gccagagccg
     5461 aaccgacacc tcaatccatg attctgggaa tcagaccgga tcaactgcct gttactccga
     5521 caaccaggaa tttcgagatt atagttgccg aatagttcag tgcaggaatt gacaacttgt
     5581 gggtaatcga tgttaatctt ttttcatggc tcttcgatgg ttttgcttac ttgagcgaac
     5641 ataggattag gagaaggtaa tgaaacgcgg gcatcattca aaaaaccacg gccaggaatg
     5701 gtccgtagca gccgcccttc tcaatggttg caacacaata gagcaattga aagattactt
     5761 ctctataatg gggagaagat tcggcatatt cttcgagatc ttcccttatg ggaaaaaagc
     5821 gcacgaagaa aaggatctag attcattttt aaataccgct attcagaaaa tgaaagagtc
     5881 cggctgggtc attcaggacg gtgaaagata tttgctgacg ggaaaaggac ggcatcaagc
     5941 aaaggagatg ctggccgaac tggaaaacag cggcagactt ctcgcccgcg caacacagcc
     6001 caaaaccgtc gcaaagatca cggtgatagc ccatttcgtt cttgcggctt tgaaactgcc
     6061 ggccgctctt ctttcaggaa gcaccggtct cctgaacgac tccttcgata ctcttctgga
     6121 cggagtctca agtatttttg tgtactgggg agttaagaag aagcgcgaaa acctggtaag
     6181 ctttattctt ctattcttca tggggataac gggaatcttt tcgctgtttg aggcattatc
     6241 gaggcttgtc tcgggtgaaa taccatctcc agatctcctc acttttgcgg cagttgttat
     6301 ctcgggtttg atctgcgcct ttctctggtt ctatcagaaa tactcgggtc tgaaaaaccg
     6361 ttctttcccg ttgatcaccc aatcaacaga ttccagaaac catgttctcg tagctgtgag
     6421 cgtatctatc gggcttattg tatcgctgat gaaagttcca tatgtcgatg cccttgtagg
     6481 aattatgatt tcccttctta ttctcaaagg tgcgacagat ctgttgatag atctcataaa
     6541 atcatcaagg ggagaggcga tggactttga gaaatatggt ttctctctct tcaacaagtt
     6601 cagggcgaaa cagctcaaac gctggtttct tttcatgatt gaacagggaa agatcaaaac
     6661 ccgcgagcag ctgatctgcg aagcaaaggc atctatggtt tatcaggata tcgaaccgtt
     6721 gagaactctg gggctttcag attcccagtt cgacgagcta attgtaaata ccgcgctgga
     6781 ggccctggac agcgaaaagc ttatctctga aacggccgga cagctctccc tgactgaaga
     6841 gggtttcagc gaactaaaat tcacccgaaa gtgaaaaaag aagaacaaac ataccaccgc
     6901 agaagactgt tgttttatcg gcttctaaag tcgccggtgc tataatcgtt ttgaattcag
     6961 acgtttcata gaatcgcttt cccgccgtgt cgaacacttc tcaaagatag aatgaaaacc
     7021 agccagataa gactcgagca gagaataaaa actttgatct gtgatcgcca ggagaaaaac
     7081 aagagcaaac agaggggtga gaagggtgaa gaaagtcctt ataacactgc tagtagttgc
     7141 tttctcggtg atggttttct ggctaaagga gatggttgtt gtagagaagg gaagtttcac
     7201 gatgggtgat
//
LOCUS       DAOOLY010000113         7081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12575_length_7081_cov_4.193994, whole genome shotgun sequence.
ACCESSION   DAOOLY010000113 DAOOLY010000000
VERSION     DAOOLY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12575_length_7081_cov_4.193994"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLD15_06855"
     CDS             <1..837
                     /locus_tag="PLD15_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB63659.1"
                     /translation="GEWVQEVIQKPVKEEELIGKIREHIDFTPIGVEESRVLVVEDNE
                     ANQFLIKKILEKAGYLVNIASNGLEATELVRREKYVLVLMDMQMPVMDGYEATRILRD
                     EGFKMPIVALTAHTMQGDEERSISAGCDGFLGKPVKQTDLIETVRYYTGIYGKTGKKR
                     ETLTLNATDGGGNRSPSDEKIEIFAREMGLTLEEASEMFEEYGKHIGNTIELIRSLLE
                     RGDLESLAREGHSLKGSGRMYGVEEISELGSKLEIAAKNLRKTELSDILSQLEKLRRK
                     LW"
     gene            879..1958
                     /locus_tag="PLD15_06860"
     CDS             879..1958
                     /locus_tag="PLD15_06860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB63660.1"
                     /translation="MRRTLFICIVVLFSSIVTANALNEVVQRVADFEGFWGAILVARG
                     DVILHAAGYGMADVERKIPNTPEKKFRIASITKQITAAAILQLAERGELTLDDPLSMY
                     IEGFPNGEKIQLRYMLNHSSGIPTMINTGELLERIRGYEDGKSLQGAEIEYISTLPLR
                     FEPGTSFLYSNSAYFLLGVVIEKVSGMPYDEYLRENIFEPLGMNSTGYDFNEYDSGWA
                     RPYYCQSYVIDNECIREADWVDRRLPGGAGGLYSTVYDLYLWNRALYGTDILSRESVK
                     IMETPASQLYEAGLGVFVGTEFIAGQYRRLVYHDGDTKGTSTRINRYVEDDIFVVVLS
                     NIEGKDFTSLAHELARAVITNDMGR"
     gene            2045..2515
                     /locus_tag="PLD15_06865"
     CDS             2045..2515
                     /locus_tag="PLD15_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPB63661.1"
                     /translation="MISERVLRVLERYDLKYREFPDGSTPTAVTAADMLGVAVGQIAK
                     SILFVCKKNGAFLVVCPGDRKVSSSKLKKVTGEKPRLASADETEALTGFLPGGVCPFG
                     LNGVKILIDEWLREYDVVYPSAGTSGSAVETTFEDLVRITGGEAFDLTNPLDQS"
     gene            complement(2506..3285)
                     /locus_tag="PLD15_06870"
     CDS             complement(2506..3285)
                     /locus_tag="PLD15_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63662.1"
                     /translation="MDDVIVIRDLRFGYSREEVLSGIDLTLKQGEVLGILGPNGSGKS
                     TLMAIICGILKNWSGSIEIMGSPLNDYAGKRLARILSYIPQSFDPSFDLKVETIVSFG
                     RNPHLKGLSGPGVRDYEVIDEVMELTEIYGFKDRFFSTLSGGEKQRVVIAKALAQEGR
                     ILLMDEFTSHLDPGHSRKVGKIATDLVRAKGLSAMAVFHDLNQAIEMSDRLVFMKEGK
                     IEALGEVWETVTPDIIWKVYGLRAQIITNPVTELPLVVFYD"
     gene            complement(3278..4270)
                     /locus_tag="PLD15_06875"
     CDS             complement(3278..4270)
                     /locus_tag="PLD15_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPB63663.1"
                     /translation="MKKILLFISIATASLIGMLWLSSFGTVKYSMFEVIQMISGSLTG
                     SRANIYMNLRLPRLLVAFLIGSGLAVGGNGLQLTLKNPLADPYIIGISAGASFGAVFS
                     LFIKEMSRTTINMDMMAFLFAVFSAFLTYTISRRGGRIPITTLVLSGVIVSSLFNAAV
                     TFLVVFAWRNVITLHFWSLGSLSGVTWGDVLKLLPAILFQYFVFTVIRRKMLILATGE
                     EHAITVGVNPERIKFVVFFTVSIVSAIAVSTAGLIGFVGLIIPHMVRLAFGTDNRLNL
                     PATALLGGFFLIVCDTFARTLFQPTELPIGAVTALVGAPVFIYLLRSKEVVRNG"
     gene            complement(4267..5151)
                     /locus_tag="PLD15_06880"
     CDS             complement(4267..5151)
                     /locus_tag="PLD15_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63664.1"
                     /translation="MKKLVLVLSVLLAISTVMAIELVDDLGRLVSIDKTPGRVVIASP
                     AITYYLELLDVTHTIVGVTDWDSFALSREVTKIGNLTPLNLEMILSLEPDLVLISGGF
                     QEAEVEKLERIGIDAFVVNPNSFEDIYRNLTVLGNIFAIPERGRNVAAEFRNGVLEVA
                     KNVYNVPQDERPKVMYVMIAGSVSDIWTCGTGSFVNQAIAYAGGANVAAPYSGNNGWF
                     PVSPEFVLNTQPDVILVPYYYEGGQREAVEMVTSYKPFAGVPAVKNGRVYPLSDSLTA
                     YANPRFIDLVKALMEYFY"
     regulatory      complement(5206..5382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5374..>7081)
                     /gene="mutL"
                     /locus_tag="PLD15_06885"
     CDS             complement(5374..>7081)
                     /gene="mutL"
                     /locus_tag="PLD15_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPB63665.1"
                     /translation="VENSIDAGASLVEVEIRSGGKEYIRVRDNGTGMTAEEARLSLKP
                     HTTSKIAAIEDLDSLSTFGFRGEALSTIASVSRMLLSTVGGEDTLGVTLEVTAGEVSG
                     EKFFKGARGTTVEVFDLLFNTPARRKFLKSVSIEGRMVTEMMQRFILSFHNVDFLYIR
                     DNQTIYDTRGLTGIEDRVLMIYPELSRRDLLPFREETREMRVEGLVTLPVRTRRNRMG
                     ENIFVNGRYVRQFELNYALERGYGEALEKGNFPFAVVFIQVDPVEVDVNIHPQKLEVK
                     FASPATVLEGLKRAVRSAIRNAGSFTIDIQPPSTGGEESTVEERDEPQIRYRERAFPT
                     DRTDRKSDEVKEPSWRNYDRNESMPLSVERRLFRPYTESRKDEALQKRVQDRTGFLGV
                     LGERYILVESGEGLLIVDQHAAHERILYERLKEKMVIDSQNLLVPVELSFEAGREELL
                     TLKRERIEQLGFNFRFENDKILLVAIPQLLPVESAVETLGEVIDELRLEGLEEPERVF
                     DNLLATIACRSAIRTGDKLDIGQARELFEELRKRQLLVCPHGRPISMVIRQEDLDRYF
                     SR"
BASE COUNT         1735 a         1855 c         1840 g         1651 t
ORIGIN      
        1 ggggagtggg ttcaggaggt catccagaaa ccggtgaaag aggaggaact gatcggaaag
       61 atccgcgaac acatcgattt cacgcctatc ggtgttgaag aaagcagagt tctcgtcgtg
      121 gaggacaacg aagccaacca attccttata aaaaagattc tggaaaaggc gggatacctg
      181 gtgaatatcg ccagcaacgg tctcgaagcg acagaactgg tgagacgcga aaaatatgta
      241 ctggttctga tggatatgca gatgcccgtc atggacggtt acgaggctac cagaattttg
      301 agagacgaag gtttcaaaat gcccatcgtc gccctcaccg cccacacgat gcaaggagac
      361 gaggagagat cgatctcggc cgggtgtgac ggcttcctcg gcaaacccgt aaagcaaacc
      421 gatcttatcg aaacggtgag gtattacacc ggaatatacg gcaagaccgg aaaaaagcgt
      481 gaaacgttga ctttgaacgc caccgatggt ggcggcaaca gaagtccctc cgacgagaag
      541 atagagatat tcgccagaga gatggggctg acactcgaag aggcttcaga gatgttcgag
      601 gagtacggaa aacatatagg gaacacgatc gagttgatcc gctcgctact tgaaagaggg
      661 gatctcgaat ctctggcgcg cgaggggcac agcctcaagg gatctggcag gatgtacggg
      721 gtagaagaga tatccgagct cggttcgaag ctagaaatcg cagcgaaaaa cctcaggaaa
      781 accgagctct cagatatctt atcgcaactg gaaaaactcc ggcgaaagct ctggtgaagc
      841 cggccaacaa ggggaacatg atttgaaagg aagtggaaat gagaagaact ttgtttatct
      901 gtatcgttgt gcttttctct tcaatagtca cggccaacgc cctgaatgag gtcgttcaga
      961 gagtcgctga tttcgaaggc ttttgggggg cgattctggt tgcccggggc gacgttatac
     1021 tccatgcggc cggttacggc atggccgacg ttgagagaaa aattcccaat acacctgaaa
     1081 agaagttcag gatagcctct ataacgaaac agatcaccgc cgcggcgata ctgcagcttg
     1141 ccgaaagagg agaactgacg ctcgatgatc ctctgtcgat gtacatagaa ggctttccga
     1201 acggagagaa aattcagctg agatacatgt tgaatcactc ttcggggatt ccaaccatga
     1261 taaacaccgg ggaattgctg gagaggatac gcggttacga agacggtaaa tctcttcaag
     1321 gtgcggagat agaatacata tccacgcttc cgctcaggtt cgaaccaggc acatccttcc
     1381 tttacagtaa cagcgcttac ttcctgctgg gagtggttat agaaaaggtt agcggaatgc
     1441 catacgacga atatctgagg gaaaatatct ttgagcccct cgggatgaac agcaccggct
     1501 acgatttcaa cgaatacgac tcgggatggg cacgcccgta ttactgccag tcgtacgtta
     1561 tcgataatga gtgcatcagg gaagccgact gggtcgatag aagactgccc ggaggcgccg
     1621 gcggcctcta ttcaacggtg tacgatctct atctctggaa cagggcattg tacggcactg
     1681 atatactatc cagagagtca gtgaagatca tggaaacgcc tgcaagccag ctttacgaag
     1741 ccggtcttgg cgttttcgtg ggaacggagt tcatcgccgg acagtacagg cgactggttt
     1801 atcacgatgg cgacaccaag ggaacctcta cccggataaa ccggtacgtg gaagacgaca
     1861 ttttcgtcgt agtactcagc aacatcgaag ggaaggattt cacctcgctg gcacatgagc
     1921 tggccagggc ggttataacg aacgatatgg gaagataata tcgatcatac agcactgcgc
     1981 atcacgctca ggagaaattc agacgctgtt tctattatgg tcgtgcaaaa gaaggaggag
     2041 gaagatgatt tccgaaagag tcctgagagt tctggagagg tacgatctga agtaccgcga
     2101 gtttcccgac ggttccaccc ccacggccgt aaccgcagcc gatatgctcg gggtcgcggt
     2161 cggtcagatc gcgaagtcga tcctttttgt ttgcaaaaaa aacggggcct ttctcgttgt
     2221 ctgtccggga gatcgcaaag tctcctcctc aaaattgaag aaagtgaccg gagagaaacc
     2281 ccgcctggcg agcgccgatg aaacggaagc tttaaccggt ttcttgcccg gcggtgtgtg
     2341 tcccttcggt cttaacggag tgaagattct gatcgacgaa tggttgagag aatacgacgt
     2401 agtttatcct tctgcgggaa cttcgggctc ggctgtggag accactttcg aagatcttgt
     2461 gaggataacc ggcggtgaag ccttcgacct gaccaaccct ctcgatcaat cgtagaaaac
     2521 caccagtggc agctccgtca cgggattggt tattatctgg gctctgagac cgtaaacctt
     2581 ccatatgata tcgggggtca ccgtctccca gacttcgcca agtgcctcga tctttccctc
     2641 tttcatgaaa accagtctgt ccgacatctc tatggcctga ttgagatcgt gaaagacggc
     2701 catggcgctc agacctttgg cccgaacgag atcggtagca atcttgccga cctttctcga
     2761 gtgtccggga tcgagatgcg aggtgaactc gtccatgagg agtatccttc cctcctgggc
     2821 gagagctttg gcgatgacca ctctctgctt ttctccaccg ctcagggtac tgaaaaagcg
     2881 gtctttgaat ccgtatatct ctgttagttc catgacctcg tctatgactt cgtagtccct
     2941 taccccggga ccggacagtc ccttgaggtg ggggttcctg ccgaaagaga ctatcgtctc
     3001 gaccttcaga tcgaaggaag gatcgaaact ctgtggtatg tacgaaagta ttctggccag
     3061 tcttttgccc gcatagtcgt tcagcggact ccccattatc tctatcgatc cactccagtt
     3121 ttttagaatc ccgcatataa tggccatcaa agtggatttt ccgcttccgt tgggtccgag
     3181 aataccgagc acttcgccct gtttgagggt gagatctatg ccgctcagaa cctcttctct
     3241 cgagtagccg aagcggagat cccttatgac tataacatca tccattcctg accacttctt
     3301 tactcctgag aaggtagatg aaaactggag cacccaccag agccgtaacg gccccgattg
     3361 gcagttcagt aggctgaaag agcgttctgg cgaaggtatc gcaaacgata agaaaaaagc
     3421 cacccaacaa cgccgttgcc gggaggttca gcctgttgtc cgtaccgaag gccagtcgca
     3481 ccatgtgcgg gattatcaag cccacgaagc cgatcaatcc ggccgtggac accgctatcg
     3541 cggagacgat cgaaaccgtg aagaagacca cgaacttgat tctctcaggg ttcaccccaa
     3601 ccgttatcgc gtgttcctct cccgtcgcca gaatcaacat ctttctcctg atcacggtga
     3661 aaacgaagta ctggaagagg atcgccggca gcagcttgag gacatctccc cacgtgactc
     3721 ctgagaggga accgagtgac cagaagtgaa gcgttatgac gtttcgccag gcgaagacca
     3781 ccagaaaggt cacggccgcg ttgaaaagcg aggagacgat cacaccggag agcaccaggg
     3841 tcgttatcgg aatccggcca cccctgcgcg agatggtgta agtcaaaaag gctgagaaga
     3901 cggcgaaaag aaaggccatc atatccatat tgatggtagt tctgctcatc tcctttatga
     3961 agagtgagaa gacggcgcca aaagaggccc ccgccgatat gcctatgata taaggatcgg
     4021 cgagagggtt cttcaatgtt agctgcagac cgttaccgcc aacggccagt ccggagccaa
     4081 tgaggaaggc gaccagaagt ctgggaaggc gcaggttcat gtagatgttc gcacgcgatc
     4141 cggtcaggct tcccgagatc atctgaatga cttcaaacat cgagtatttt acggttccga
     4201 aactcgataa ccacagcatc ccaatcaggg atgccgtggc gatcgatatg aaaagcagta
     4261 tcttcttcaa tagaagtact ccatcagagc ctttacgaga tctatgaatc tggggttggc
     4321 gtaggcagtg agcgaatctg agagagggta aaccctcccg ttcttcactg ccgggacgcc
     4381 ggcgaagggt ttataggacg ttaccatctc tacggcctcg cgctgtccac cctcgtagta
     4441 gtagggaacc aggattacat ccggctgcgt attgagtacg aactccgggc tgacggggaa
     4501 ccagccgttg ttgccactgt aaggcgcagc aacattggca cccccggcgt atgcgatcgc
     4561 ctggttcaca aaagaaccgg taccgcaggt ccatatatcg ctcaccgaac cggctatcat
     4621 tacatacatc accttcggtc tttcatcctg aggaacgttg taaacgttct tggcgacttc
     4681 aagcaccccg tttctgaact ctgcggccac attccttccc cgttcgggaa tcgcgaagat
     4741 attgccgaga acggtaagat tgcggtagat atcttcgaaa gagtttggat tgaccacgaa
     4801 cgcatcgatc cctatcctct cgagcttttc cacttcggct tcctgaaagc ccccggagat
     4861 gagtaccaga tcgggttcaa gagatagaat catctcgagg ttgagagggg tgaggttccc
     4921 tattttggta acttccctcg aaagggcaaa ggagtcccag tcggttacgc ctactatggt
     4981 gtgggtgacg tccagaagtt ccagatagta ggttatcgcc ggagaggcga tcacgactct
     5041 cccgggagtc ttatcgatcg aaactagacg cccaaggtca tcgaccagtt cgatggccat
     5101 aaccgtagag atcgcaagaa gaaccgataa caccagaacc agttttttca taaaaactct
     5161 cccctttttg caaggaattt caccatcctt ctgcctcgga aggatggtga tatctcaact
     5221 ccgctcaggt ctcctgactc acggctcaac ctaatctcca cgccttccca gtttcccagt
     5281 ggcaccttgt ggatttcgta accgttcaca gtggcgggac cgtaccggac tctcaccggt
     5341 ttcccttgat gcggagttac aaagtcattg gaactatctg gaaaaatacc tgtccagatc
     5401 ctcctgtcgt attaccatgg atatcggtct tccatgggga cagacgagca actgcctctt
     5461 cctgagctct tcgaaaagct ccctggcctg tccaatgtca agcttgtctc cagttcttat
     5521 ggcggatcta caggcgatcg tcgcgagaag gttgtcgaag actctttccg gttcttcgag
     5581 tccttccaga cgtaattcgt cgatcacttc cccgagagtt tcgaccgcac tttcaacggg
     5641 cagcaactgg ggtatcgcca ccaacaggat tttatcattc tcgaacctga aattgaagcc
     5701 gagttgctcg atcctctctc ttttcagggt gagaagctct tctcttccgg cctcgaaaga
     5761 gagttcgacg ggtacgagta gattctggga atcgataacc atcttctcct tcaatctctc
     5821 gtacaggatt ctttcgtggg cggcgtgctg atcgacgatc aacagtcctt cacccgattc
     5881 gaccagtatg tacctctccc cgaggacgcc gagaaagcct gttctgtcct gaacacgctt
     5941 ttgaagagcc tcgtcctttc tggattcggt gtagggtctg aaaagccgac gctccacact
     6001 cagcggcatg ctttcgtttc tgtcgtagtt tctccaggaa ggctctttaa cctcatcact
     6061 ctttcggtcc gttcgatctg tcgggaaagc tctctctctg tagcggattt gaggttcgtc
     6121 tctctcttct accgtcgatt cttccccgcc ggttgaggga ggctgaatgt ctatggtgaa
     6181 gctcccggca tttcttatcg cggaccttac cgctctttta agaccttcaa ggaccgtcgc
     6241 cggagaggcg aacttcacct ctagcttctg cgggtggata ttgacatcga cctcgaccgg
     6301 gtctacttga ataaaaacga ccgcgaaagg gaaattaccc ttctccaggg cctctccgta
     6361 ccctctctcg agggcgtagt tgagctcgaa ctgcctcaca tacctgccgt tcacgaatat
     6421 gttctctccc attctgttgc gtctcgtcct taccggaagc gtgacgagtc cttcgactct
     6481 catctcgcgc gtctcttctc tgaacggtag taggtctctc cttgaaagct cggggtagat
     6541 catcaagaca cgatcttcga tacctgtcag accccttgtg tcgtaaattg tctgattgtc
     6601 gcgtatgtat aagaaatcaa cgttatgaaa ggatagtatg aatctctgca tcatctcggt
     6661 gaccatccgc ccctcgatag agacggattt aagaaacttc cttctggcag gcgtgttgaa
     6721 gagaagatcg aagacctcca cagttgttcc cctcgcccct ttgaaaaact tctctccgct
     6781 cacttctccg gccgtaactt ccagcgtcac tcccagagtg tcctcaccgc ccacagtgga
     6841 gagcagcatt ctggaaacgg aggcgatggt ggaaagcgct tcgcccctga agccgaaggt
     6901 cgaaagagaa tcgagatctt cgatagccgc gatcttgctc gttgtgtgag gtttcagaga
     6961 gagcctggcc tcttcggccg tcatccccgt gccgttgtcg cgcaccctta tgtactcttt
     7021 tccaccggat cttatctcga cctccacaag cgatgcgcct gcatcgatgc tgttttcgac
     7081 c
//
LOCUS       DAOOLY010000114         7045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12644_length_7045_cov_4.027611, whole genome shotgun sequence.
ACCESSION   DAOOLY010000114 DAOOLY010000000
VERSION     DAOOLY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12644_length_7045_cov_4.027611"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLD15_06890"
     CDS             complement(<1..271)
                     /locus_tag="PLD15_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB63666.1"
                     /translation="MAYMTVNGVNIYYELEGKKDTGETVAFFNGVMASTSSWASQRKL
                     FEKFGFKILLHDFKGQLLSEKPAGPYSFAQHALEARLLMKELGIER"
     gene            complement(304..1089)
                     /locus_tag="PLD15_06895"
     CDS             complement(304..1089)
                     /locus_tag="PLD15_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63667.1"
                     /translation="MRQIRSIMGVLSVYMRSFVDVMSDRSLMNRSLFSSNLSIYRALK
                     WFNIAFSFLLFIPVFASLVSFNLPHLLGYLIMAFLGYATWIFQGITGVPIFEEIRFLA
                     LLHLNLHSVFGMWLEFYNRFVIYDDILHIHGGFLGAVAIFPFVLGSSLAWSNLPERAV
                     KWKVWFSALSIVNMFGVFWEIFEFISDRIFRNYPGYRMAQENSLNDTMLDLIYNNIGA
                     SIGILVFWWYLRKSRDVNAFMEKMGRKLGEFLETGRKRVRTEN"
     gene            1296..1652
                     /locus_tag="PLD15_06900"
     CDS             1296..1652
                     /locus_tag="PLD15_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB63668.1"
                     /translation="MIEVKGNKYYDTKEVAEILSCTPWTIRKWINDGTLKATKLGKRY
                     FVDEWTIQKKLDWNAKGGEETEEPCTSGKKHSGTLTEIERKAAQFKELPDGRYAILDG
                     EGNRIGTLIKEENDLH"
     gene            1951..2811
                     /locus_tag="PLD15_06905"
     CDS             1951..2811
                     /locus_tag="PLD15_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPB63669.1"
                     /translation="MEKSFLSPSENAVSFIETGCKKTSLTAAKEFVLGILGGAFIAFA
                     AEGSNVAIHTIGSVGLAKALAGAIFTTGLMMVIIAGAELFTGNSLIVISCLKSKSRWL
                     ALLKNWFFVYTGNFVGSLLVVYFILHSGQLGFSGNLLGGFTIKTAVYKTGLSFENAFF
                     MGILCNWLVCIAVWMASTARDIAGKVLAIFFPIWLFIASGFEHSVANMYYIPAGILAK
                     ANEGFVQAAISLGVKSDQIVNLNWGTLFTKNLIPVTLGNIVGGALFVATIYWFVYLRD
                     GKAKGDKSIA"
     gene            complement(2923..3696)
                     /locus_tag="PLD15_06910"
     CDS             complement(2923..3696)
                     /locus_tag="PLD15_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPB63670.1"
                     /translation="MKILIIGGGSIGGSMALRLCERGCRVSVFDTCEKILSTLFYSGI
                     ETGPLDLLLPGNEITVIATPMSVELELLESMEFNGPVMDVASVMRPFREVASRRKIRF
                     INGHPMAGNEHSGPAGWDRRMFEGRPFFLSPCGMARNSDVSTALKIVNDLGARPEFIE
                     PAEHDKMLSRISQGLYYLSRVAMKLGKNHEKYAGPGFASTTRLGRQNREMVLDMARYN
                     GDNIAEALEKIEEYLHKVKEAIRSKDMMELVKLIDLEEI"
     gene            complement(3693..4052)
                     /gene="aroH"
                     /locus_tag="PLD15_06915"
     CDS             complement(3693..4052)
                     /gene="aroH"
                     /locus_tag="PLD15_06915"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPB63671.1"
                     /translation="MKAIRGATSVENNEREEITARTIELMEEIFAKNDIWEIHSVIFT
                     VTRDVTAFNPATAFRQRFDYSDVALLCINEADFQGSSNGIIRVLIHCESTSKNFVYLH
                     RARDLRPDLVRKDEDNP"
     gene            complement(4074..5423)
                     /locus_tag="PLD15_06920"
     CDS             complement(4074..5423)
                     /locus_tag="PLD15_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPB63672.1"
                     /translation="MEFLSVPLPEKIIKHESEGAFEKATREIDFQLSLSLPSILRERL
                     LWERERLRRIRENYIYTIDIAFEILDSELADFTRAEFDELKKGGYLDFMVIDGEEFYE
                     KRFDKNLLFARNDYEKRLKEKDIERNKKKELLQTQIDRLISERVPATFRVRARSSMKA
                     RVQAGDKGEYLKCWLPVARPGDQIENVKILNTSQKNYFLAPPDAPQRTIYMEAPMGEV
                     NFSVEFEYEISEISTSIDPFSIRPVDEARFYHYLRERAPHILFTPFLKSLASEIAGDE
                     RNPYYIAKSFYDWICDNVRYSFMSEYALYGNLSEFAASNMRGDCGLKALLFMTLCRIK
                     GIPSRWQSGWYATPAGASPHDWAFFWIEPYGWIPVDGSFGGARKNIPAYREFYFGNLD
                     AYRMVANSAFMAPLTPSKNFYRFDPYDNQTGEIETNLRPIRSDEKEHTIEILDFERI"
     gene            complement(5484..6128)
                     /locus_tag="PLD15_06925"
     CDS             complement(5484..6128)
                     /locus_tag="PLD15_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPB63673.1"
                     /translation="MILGICGNIGAGKSSLTALLEERLGFRGVYEAVDENPFLKDFYS
                     DMGRWAFHSQLFFLIKRFDFLKRVNTEGAVVLQDRTIYEDVKIFARNLHLMGYIDSRD
                     WELYMDTFKTLSDHLSTPTGLVYIKCSTPVLMKRIKKRGRGFESDIKEEYIERLNRLY
                     DEWIEDADFCGKLIIDGDKYDFMESDQDRDDVLHLISRFNAELVAGVQSKLFSR"
     gene            complement(6125..6739)
                     /locus_tag="PLD15_06930"
     CDS             complement(6125..6739)
                     /locus_tag="PLD15_06930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPB63674.1"
                     /translation="MFSIASGKYIAVEGVIGVGKTTLVRNLSLKYSIPTVLEVVEENP
                     FLPNFYEDMDRWAFQTQLFFLVSRFDQQSAVKRTAAQGQGVVSDYTFYKDHLFASLTL
                     KGDQLELYEKIFKVLQDQVQTPDLVVYLYADVNVLMSRIALRDRPFERSMDRNYISML
                     SEAYEEHMGSERNFPVLRIDTSAIDFVRNQKDLHEIFERIEASL"
     gene            complement(6860..>7045)
                     /locus_tag="PLD15_06935"
     CDS             complement(6860..>7045)
                     /locus_tag="PLD15_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63675.1"
                     /translation="VADSSLCLYRVAAGTIRESLILCIPGYHKSVANYLDPVIDVLKP
                     LFDGWKERAEASGEENA"
BASE COUNT         1809 a         1687 c         1618 g         1931 t
ORIGIN      
        1 ccctttcgat cccaagctct ttcatcagga gtctggcttc tagtgcatgc tgggcgaagg
       61 agtaaggtcc ggccggtttc tctgaaagga gctgcccttt gaagtcgtgg agcagaatct
      121 tgaagccgaa tttttcgaac aacttcctct gcgatgccca gctgctagtt gaagccataa
      181 cgccgttgaa gaatgccaca gtctctccgg tgtctttctt tccttccaat tcgtagtaaa
      241 tattcactcc gttcactgtc atatatgcca taaagaaaac ctcccgtggt cagcaatacg
      301 atttcagttc tccgtcctca ctctctttct accggtctcc aggaactctc caagcttgcg
      361 acccattttt tccatgaagg cattcacgtc tcttgatttc cttaaatacc accagaagac
      421 tagaatgcct atcgaagcgc cgatgttgtt atagatcaaa tctagcatgg tgtcgttcag
      481 actgttctcc tgggccatcc tgtagcccgg gtagtttctg aatatcctgt ccgaaatgaa
      541 ttcgaagatc tcccagaaaa ccccaaacat gttcacgatc gagagagctg agaaccagac
      601 cttccacttg acggcgcgct cgggcaagtt cgaccaggca agggatgacc ccagaacgaa
      661 gggaaaaatg gcaactgcac ccaggaaacc tccatgaatg tgcaaaatgt cgtcgtatat
      721 aacaaatctg ttgtagaact ccagccacat accgaacaca ctgtggagat tgaggtgcag
      781 cagtgccaga aatcttatct cttcgaagat tggaacgccg gttattcctt gaaagatcca
      841 tgtcgcgtaa cccagaaagg ccataatcag atagcccagc agatgcggaa ggttgaagct
      901 caccagcgac gcaaaaaccg gtatgaagag gagaaacgaa aaagcgatgt tgaaccattt
      961 caaagccctg taaatactta aattactgga gaaaagcgat ctgttcatta agctcctgtc
     1021 ggacatgaca tccacgaagc tgcgcatata gacgctcaaa actcccatta tagacctgat
     1081 ctgcctcacc gaatcacctc cactgtgtct ggagaccgct agctgtatta tatatcgcaa
     1141 ttgtttttag ggcgaaacaa gcttttggca tcaccatgtc tgagaactcc attttttagc
     1201 tctatcctaa aacaaagcct atgaatatgt ttagattaaa taagattgat atatattaga
     1261 ataaatggta atatattgaa gaggagggga atataatgat agaagttaaa ggaaacaaat
     1321 actacgacac aaaggaagtg gcagaaattc tgagctgcac accctggacc atccggaaat
     1381 ggataaatga cgggactttg aaggccacca agctggggaa aaggtacttc gttgacgagt
     1441 ggacaatcca gaaaaagctg gactggaacg ccaaaggagg cgaagaaaca gaagaacctt
     1501 gcacaagcgg gaaaaaacac tccggaacac tgaccgaaat cgaaagaaag gccgctcaat
     1561 tcaaagagct tcccgatggc agatatgcaa tccttgacgg ggaaggcaac aggataggta
     1621 cgctaattaa agaggaaaat gatctacatt gacggatgtt tatgggattg agctccttat
     1681 tagaatagag aagcgcaaca aaagacatga aaccgggaaa ggcggataga ttatacgata
     1741 aagaatagtt cttctcacct aacggattta acctattggg tgacctgacc cttcttctct
     1801 ttgtcactac aaattgaatt aattgagact atcctttctt cataggctgt ggtaacattt
     1861 tgtcaggttt gctaaatgta tttcgatctg caaaatcacc tgttttgctg gaaatttcct
     1921 atcatattaa tcgcaggggg aagtgtgtgt atggaaaagt cttttctgag tccttcggaa
     1981 aatgctgtga gtttcattga aacagggtgc aaaaaaacca gtttaacggc cgcaaaagaa
     2041 tttgttctgg gtattctagg gggtgcgttc atcgcctttg ccgcagaggg gtccaatgtt
     2101 gcaattcaca caatcggctc tgtcggattg gcaaaggccc tcgccggagc gatattcacc
     2161 acgggactga tgatggtgat tatagccggt gccgagttgt tcacaggaaa cagtcttatc
     2221 gtcatttcgt gtttgaagag taagtcaaga tggctggctt tgttgaagaa ctggttcttt
     2281 gtatatacgg gaaactttgt agggtctctt ctagtagtat atttcatact acattccggt
     2341 cagctgggtt tttccgggaa tctactggga ggattcacta tcaagacggc cgtttataaa
     2401 acaggtctga gctttgaaaa cgccttcttc atgggtatat tatgtaactg gctggtctgt
     2461 atcgccgtgt ggatggcctc cacggccaga gatatagccg gaaaggttct tgccattttc
     2521 tttccgatct ggttattcat agcatctggt tttgaacaca gcgtggcaaa catgtactat
     2581 atacccgcgg ggattctcgc caaagccaac gaaggtttcg tccaggcagc catctcgttg
     2641 ggcgtcaaga gcgatcagat agtaaacttg aattggggaa ctctcttcac taaaaacctc
     2701 attcctgtga cgctcggcaa catcgtaggt ggcgcactgt tcgttgccac aatctactgg
     2761 ttcgtgtatc tgcgagatgg gaaggcaaag ggtgataaaa gtatcgctta gttctccgga
     2821 gaatcaccgg ttgatgtttg aaatataagt caatcacaat ctcaaattga gctgcgtcat
     2881 ggtactctct gaagtgctag atgcagtgta tggtgtgttt ttctatattt cttcaagatc
     2941 tatgagtttg acgagttcca tcatatcttt agatcgtatc gcctctttga ctttgtggag
     3001 gtactcttcg attttttcaa gagcttcggc gatgttgtct ccattgtatc tggccatatc
     3061 cagtaccatt tctctgttct gcctgccgag tctggttgtg gaagcaaaac ccggaccggc
     3121 atatttttca tgattctttc ctagtttcat tgcgacccgg gacagataat aaagcccctg
     3181 gctgatacgc gagagcatct tatcgtgttc ggccggttct atgaactcag gtctggctcc
     3241 caggtcgttt actatcttca acgcggtgct gacgtcgctg tttcttgcca ttccacacgg
     3301 agagagaaaa aaggggcgcc cttcgaacat tcttctgtcc cagccggccg gtccagaatg
     3361 ttcgttgccg gccatcggat ggccgtttat gaagcgtatc ttccttctcg acgccacttc
     3421 acggaagggc ctcatcacac ttgccacatc catcacagga ccattgaact ccatactttc
     3481 gagtagttcc agttccacgc tcatgggagt agctatcacg gttatttcat ttcccggtag
     3541 caacagatca aggggaccag tctcgatccc gctgtaaaag agcgttgaaa gtatcttttc
     3601 gcatgtatcg aagacagaga cacggcaacc ccgctcacaa agtctgagag ccatcgatcc
     3661 accgatcgat ccgcccccga ttataagaat cttcatgggt tatcctcatc tttcctaacc
     3721 agatcgggtc tgaggtcacg tgctctgtga agatagacga agttcttcga ggttgattca
     3781 cagtggatga gaacccttat aattccattc gatgatccct ggaagtctgc ctcatttata
     3841 cacaaaagcg cgacatcgct ataatcgaat ctctgcctga aggccgtagc tggattgaag
     3901 gcggtgacat ctcgcgttac ggtgaagatc accgagtgta tttcccaaat atcgtttttg
     3961 gcgaagatct cctccatcaa ctctatagtc ctggcagtga tctcctcacg ttcgttattt
     4021 tcaaccgatg ttgctcctct tatcgctttc atctcttacc cccgcgatga aacttatatc
     4081 ctttcgaaat ccagtatctc gattgtgtgt tctttttcat cgcttcgaat cggtctcaga
     4141 ttggtttcta tctcacctgt ttggttgtcg tacggatcaa atctgtagaa gttctttgac
     4201 ggtgtcaggg gagccatgaa ggccgagttg gccaccattc tgtaagcatc aagatttccg
     4261 aagtagaact ccctgtaagc cggtatattt ttgcgggcgc ctccgaaaga gccatccacc
     4321 ggaatccacc cgtaaggctc gatccagaaa aaagcccagt cgtgagggct agctccggcc
     4381 ggggttgcgt accagcccga ttgccacctg gaagggatac ctttgatcct gcaaagtgtc
     4441 ataaagagga gggctttaag accgcagtca cctctcatgt tgctggcggc gaactccgac
     4501 aggttgccgt agagggcgta ttcgctcatg aaagagtacc tgacgttgtc acatatccag
     4561 tcgtaaaagc tttttgcgat gtagtaaggg ttcctctcgt cgcctgcgat ctcacttgcc
     4621 agactcttga gaaagggtgt gaaaagtatg tgaggggctc gctcacggag atagtgatag
     4681 aaacgggctt catcaacggg tcggattgaa aaggggtcga tagaggtcga gatttccgag
     4741 atttcgtact cgaactcaac cgagaagttg acttccccca taggtgcttc catgtatatg
     4801 gttctctgcg gggcatcggg cggggccaga aagtagttct tctggctggt gttgagtatc
     4861 ttgacatttt cgatctggtc gccgggccgt gcaactggca gccagcactt aaggtactcg
     4921 cccttgtctc ctgcctgaac tctggccttc atggaagacc gggctctgac tctaaaggtg
     4981 gccggtactc tctcggatat caaacgatct atttgggtct gcagcagctc ttttttcttg
     5041 ttacgctcga tgtctttctc tttcaagcgt ttttcataat catttctggc aaagagcagg
     5101 tttttatcga accgcttttc atagaactct tcgccgtcga taaccatgaa atcgagataa
     5161 ccacccttct tcaattcatc gaactctgcg cgagtgaagt ccgccaattc cgaatctagt
     5221 atctcgaaag ctatatcaat ggtatagatg tagttctcac gtatccgcct caacctctcc
     5281 ctttcccata gcaacctttc acggagtatt gaaggaaggc ttaaggagag ttgaaagtct
     5341 atctcccggg ttgccttttc aaaagcacct tcgctctcat gttttattat cttctctggc
     5401 agtggaacag atagaaactc catacgaacc tcccgattat atgagattta cggttacagc
     5461 caatatattt acaatgagct cctctatctg gagaagagtt tcgactgtac gccagccacc
     5521 agctcggcgt tgaatctgga aatcagatga agaacatcgt ccctatcctg atcactctcc
     5581 atgaagtcgt atttgtcgcc gtctataatc agttttccgc agaagtctgc gtcttcgatc
     5641 cattcgtcgt acagtctgtt gagcctttct atgtactcct cttttatatc gctttcgaag
     5701 cctctaccgc gtttcttgat cctcttcata agcacaggag ttgagcactt gatatacacc
     5761 agcccggtcg gagtactgag gtgatcggag agtgtcttga aagtatccat gtacagttcc
     5821 cagtcgcgac tgtctatata gcccatcaag tgaaggtttc ttgcgaagat tttaacatct
     5881 tcgtatatgg tcctatcctg aaggacgacg gctccttctg tatttaccct cttgagaaag
     5941 tcgaaacgtt ttataagaaa gaagagctga gaatggaaag cccagcgtcc catatcagaa
     6001 tagaagtctt tcagaaaggg gttttcatct accgcttcgt acactcctct gaaaccgagt
     6061 ctctcttcca gaagagccgt tagcgaagac tttcccgcac ctatgtttcc gcaaattcca
     6121 agtatcataa gctcgcctcg attctttcga aaatctcgtg taagtctttc tggttcctca
     6181 cgaagtctat ggcggaggtg tctatcctca acaccggaaa gtttctttca gagcccatgt
     6241 gttcctcgta agcctcgctg agcatagaga tgtagttccg atccatgctt ctctcgaagg
     6301 gtctgtccct gagggctata cggctcatta gaacgttgac gtctgcatag agatagacca
     6361 ccaagtcagg tgtctgtacc tgatcctgca aaactttgaa gatcttttcg tacagttcta
     6421 gctggtcacc tttcagagtc agcgaagcga agaggtgatc cttgtaaaaa gtgtagtccg
     6481 aaactactcc ctgtccctgg gcagccgtcc ttttcacggc cgattgctga tcaaacctgc
     6541 taacaagaaa aaagagctgc gtctggaagg cccatctgtc catatcttca tagaagttgg
     6601 gcaagaaagg attctcctcg actacctcca ggacggtcgg tatcgagtat ttcagagaga
     6661 ggttcctgac aagggttgtc tttccaacac caatcacacc ttcaacggcg atatacttcc
     6721 cgctagcgat tgaaaacata ttttattcct acgcacaaca cttccggaaa gtctccggga
     6781 ggcaaatggc gcttctcctt tcacagttgt cttgaagact cagaccggat tcagatgttg
     6841 taacccatta aaatcgagtt caggcattct cctcgcctga agcttcggct ctttctttcc
     6901 agccatcgaa tagcggtttc aaaacgtcta tgaccggatc gaggtagttg gcgaccgatt
     6961 tgtggtaacc aggaatgcag agtatcaaag attccctgat agttccagcc gctaccctgt
     7021 aaaggcatag cgaagagtcg gcaac
//
LOCUS       DAOOLY010000115         7013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12721_length_7013_cov_5.096723, whole genome shotgun sequence.
ACCESSION   DAOOLY010000115 DAOOLY010000000
VERSION     DAOOLY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12721_length_7013_cov_5.096723"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLD15_06940"
     CDS             <1..201
                     /locus_tag="PLD15_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPB63676.1"
                     /translation="LDPVSAQKVMELTQRINREYDVTIVMITHNMEFANAYGERLLRL
                     IDGRVAQDGVRDLKVRAVAALC"
     gene            complement(193..1305)
                     /locus_tag="PLD15_06945"
     CDS             complement(193..1305)
                     /locus_tag="PLD15_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPB63677.1"
                     /translation="MTRTVTVVGAGPAGLAAARILGREGIDFTLFDSKTFPRYKGCGG
                     GLTPRSIRVLGEIFPEIEPEGFETRELSFRSIDEGVARDMVTYMSKRPLFFTVDRKEF
                     DHSLLKSVIEREGDFVVSRVREIEKTDCGFLVKGENLEIESKFVIVAGGVFGAKLVNL
                     EPPEYGIIYHGYSSPTGNASITFIQDGYLWKFPGESFDSIGGGVYPDAPNILAYEEMA
                     TLVKKTFDEDVELMGTPMPLFNTEKVLELNRAFEGLFFAGDSAGLVDNWTGEGIDFAL
                     DSGRQAALSIVEDGIKGKTAGQRYLERLYPIVRHLQVADAFRRRFHANLQGNVLLLKS
                     RRSARLFVNYISRYAGNLSLMVLKSFFTGGIRHRST"
     gene            complement(1298..2458)
                     /locus_tag="PLD15_06950"
     CDS             complement(1298..2458)
                     /locus_tag="PLD15_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63678.1"
                     /translation="MDIKRVFSSISSYAFFLFGFTSIILGSALPAIEKSFGIDHQIAG
                     ILLSLPTIAFMFSAIVVSTLSNRVGPFKLLTMGISFIAGSLVLLSIGRSFWLLLIGSM
                     ALSFGTGATETSIGIGVSGLKYRKPGSALNLMHSLFAVGSIISPFIVAAFLVDYNSWW
                     KPFFVALFAAVPLLFFTPMLYRIPYTGTDEKISRYKKELFSQKIFWLILAGVFLYVAY
                     EIGFTSWLSSFVFESKGIEMRYASIFPAILWSGILVGRLVAGFVVEKLGYELSLLFMT
                     VISFLSFGAALLMKSAVWIGVAVIFAGFGFSGTFPTLQAILISRMKNGIAFALGLFTV
                     ASSLGGAMANYLVGLLGQIFGMYAGIIFILVLIAFEMVVALMIMRLRVKTND"
     gene            complement(2624..3889)
                     /locus_tag="PLD15_06955"
     CDS             complement(2624..3889)
                     /locus_tag="PLD15_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63679.1"
                     /translation="MKDLKRVLFTLLVTAVFLCFTDTAVATGRIMPEDFEYLGAFLVP
                     QWIDGTPDAESWEWGGMSMTYDPSGDPKGKKDGFPGSIYGTGHDVWNLVSEIDIPVPV
                     ISPTRSISDLNTARTLQPFADVKDGLFAWAEEMPRVGLEILEPQGAQSSRKLYLCWGA
                     HFQDEYFYTHMWCETDLDDPRPAGAWRIEGINPYNGNDYLFAIPSEWADLYTPGMRLA
                     TGRYRDGGWSGFGPTLAAIGPWNQGNPPPDGTTLQSVVLIKYSDYFEGEPEPWYQMNG
                     YAHSDEWTGGAWLTAGDRSAVVFVGTKGMSEAWYGDTLRECMDDCELPYLRGWWSLYF
                     EGWFLFYDTSDLAKVAQGVLEPYQVQPYAHLNVDDVLYYVHGKQEKYHLGACCYDREN
                     RLFYVFEPGRESPSGEGERTIVHVWRIKE"
     gene            4295..6037
                     /locus_tag="PLD15_06960"
     CDS             4295..6037
                     /locus_tag="PLD15_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HPB63680.1"
                     /translation="MKRIVLTFFCLLLLVGIGFSVITASVGKLQASNTGMVAAGNPYA
                     AAAGAEILEKGGNAIDAAIAVSLALGVVEPYASGIGGEGYAVVSFNGEKIAVDFRSSA
                     PGLASIATAAELGLKASDFRTGPLSICTPGVLAGIIELWLYGGSLPFAELIAPAIRLA
                     SEGFEVNYTFASSCGGSYDVLLENAPGFLKEGLFAWEAGDIATNPELAETYKYLAENG
                     PQAFYNGTLADKIDALMQSRGGLLRKSDLVNYRALVKTPLHGTYREYDLFVPGIPVGG
                     PYLLETLNILEHFNLSAFAWDDPLVMNIINQALTFAMIDLLSYSGDPAYVYVPVETLI
                     SKEYSKTRFMNIDLNQALDSALYYTDYIGKPHLFEDTDSYVEALLAEAGVIELVPSTA
                     DVGQDTTHFAVIDRNGNAVAWTQTLSGFFGSGVYLDGFFINNEMGNFTLDETSRGLVP
                     GKRPVTTICPTIIEKDGKVVYLIGTPGGYYIQSTITNVIVDLIDFGMTLDEAQMTPKL
                     IGSPNYPEFRMETGFPESSRLFLEKIMGHKVREFQFPYRSFGSLNLIQVQPDGLMIGI
                     GAFRREGGASAPEF"
     gene            6102..6614
                     /locus_tag="PLD15_06965"
     CDS             6102..6614
                     /locus_tag="PLD15_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63681.1"
                     /translation="MKKLLVILLILMGTSLSLAVFFRPDIRPGYGVTETKWLSYYFEP
                     LEGTNMDTVVYFMDSGKAGPTFLLMGGTHAMEIAGTVAATIFIENAIVERGRVIVIPF
                     SNSSGASIATVFYPNQPDHFLQIQSKSGPRFLVYGDRNTDVTTRAGPIRKCISTILRV
                     KNYPVTSRET"
     gene            6674..>7013
                     /locus_tag="PLD15_06970"
     CDS             6674..>7013
                     /locus_tag="PLD15_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63682.1"
                     /translation="MELIKKENVDFNLDMHESDTPDNYDIVNGVKVPGGRLAYMLVCH
                     QRGMEIGALATMEIEFNYGVSLKLEESNIQFRGLSHREIGDATGSISFLVESPNPGFD
                     FIWREKPDTLY"
BASE COUNT         1750 a         1887 c         1808 g         1568 t
ORIGIN      
        1 ctggatcccg tcagcgcgca gaaggttatg gagctgaccc agaggataaa cagagagtac
       61 gatgtcacga tagtgatgat aacccataac atggagtttg caaacgccta cggcgaaagg
      121 cttttgaggt tgatcgacgg aagagtagcc caggacggcg tcagggacct caaggtgagg
      181 gccgtcgccg cgctatgttg atctatggcg tattcctcct gtaaagaagc tcttgaggac
      241 cataagagat aaatttccag cgtaccggga aatgtaattc acgaaaagtc tggcggaacg
      301 cctgctcttg agaagaagaa catttccctg aaggttggca tgaaatcgtc ttctaaaggc
      361 atctgcaacc tggagatgcc tcacgatcgg gtacaacctc tccagatatc tctgaccggc
      421 ggtcttccct ttgatcccgt cctccacgat cgacagggcg gcctgccttc cggaatcgag
      481 ggcgaaatct atcccctctc ccgtccagtt atccaccaga cccgcgctgt cccctgcaaa
      541 gaaaagcccc tcgaaagccc tgttcaattc gagaaccttc tccgtgttga agagcggcat
      601 aggagtgccc ataagctcta cgtcttcatc gaaggtcttt tttaccagcg tcgccatctc
      661 ttcgtaagcc agaatgttcg gcgcatccgg atagacgccg ccgccgatcg agtcgaagga
      721 ttctccggga aacttccaga gatacccatc ctgtataaag gttatggatg cgtttcccgt
      781 cggtgagctg taaccgtggt agattatccc gtattcggga ggttccagat tgaccagttt
      841 cgctccgaac acacctccgg caacgataac gaacttgctt tcgatctcga ggttttcgcc
      901 tttgactaga aaaccgcagt cagttttttc gatctctctg actctggaaa cgacgaaatc
      961 accttctctt tcgatcaccg atttgagaag agagtgatcg aactccttgc gatccaccgt
     1021 gaaaaacaga ggacgcttcg acatatacgt caccatatct ctggccacac cctcgtctat
     1081 gcttctgaaa gagagctctc tcgtttcgaa gccttcgggt tcgatctccg ggaagatctc
     1141 tcctagaact ctaattgagc gtggagtcag accacctcca cagcccttgt aacggggaaa
     1201 ggttttcgaa tcgaaaagag tgaaatcgat accctccctg ccgagaatcc tggcggcggc
     1261 caaaccggcc ggtccggcgc caaccacggt aaccgttcta gtcattggtc ttcaccctca
     1321 atctcattat catcagcgcc acaaccatct cgaacgctat cagtactagt atgaatatta
     1381 taccggcata catgccgaag atctggccca ggaggcctac cagataattg gccatggccc
     1441 cgccgagcga agaggcaacc gtaaaaagac caagtgcaaa ggctattccg ttcttcatac
     1501 gcgatatcag aatggcctgc agggttggaa aggtaccgga aaaaccgaat ccggcgaaga
     1561 ttacggccac gccgatccac accgcggact tcatcaacag cgctgcgcca aaagacagaa
     1621 acgagatgac cgtcataaac aagagggaga gctcataccc tagtttttcg actacaaagc
     1681 ccgcaaccag tcgcccgacc aggataccgc tccagagaat cgccggaaag atactggcgt
     1741 acctcatctc tatgcctttc gactcgaaca cgaaactcga aagccaggaa gtaaaaccta
     1801 tctcgtaggc tacatacaga aaaactccgg ccaggattag ccagaagatc ttctgcgaaa
     1861 atagttcctt tttgtagcgc gagatctttt catccgttcc cgtataagga atcctgtaca
     1921 gcatcggcgt gaaaaagagt agaggaacgg ccgcgaagag ggcaacgaag aagggcttcc
     1981 accaggaatt gtaatctacg agaaaagcgg cgacgatgaa tggagaaatg atcgaaccga
     2041 cggcgaacag tgaatgcatt agattcaaag cgcttcccgg tttccggtac ttcagaccgg
     2101 agaccccgat accgatcgaa gtctcagtgg cgccggtccc gaagctcaga gccatgctac
     2161 cgatgagtaa cagccagaaa cttcttccga tcgagagcag aacaagcgag ccggcaatga
     2221 aagaaatacc catcgtaagc aacttgaacg gccccacacg gttagaaaga gtgctcacca
     2281 cgatcgccga aaacatgaac gcaatagtcg gaagcgaaag cagaattcca gcgatctggt
     2341 gatctatgcc gaaactcttc tcaatagccg ggagtgcgct tccgagtatt atcgaagtga
     2401 aaccgaataa aaaaaacgcg tacgacgaaa tcgagctgaa aaccctcttg atatccatga
     2461 ttgaattcta tcacgtcgct gcaattgatg acatacaagc cagtggcaga agtttcccac
     2521 atgaactcga aaaagagaga gcgagtggag ctataaccct ggagccgtat tcgattaagc
     2581 tatctcattc gggcgaaaag gtcctcttcc ggatagtccg tttttattcc ttgattctcc
     2641 atacgtggac gatcgttctt tcgccttctc ccgaagggct ctcgcgaccg ggctcgaaga
     2701 catagaagag tcgattttcg cgatcgtaac agcaggcgcc cagatggtat ttctcctgtt
     2761 ttccgtgaac gtaatagagc acgtcgtcaa cgttcagatg ggcgtatggc tgcacctgat
     2821 agggttccag cactccctgc gccactttgg ccagatccga cgtatcgtag aagaggaacc
     2881 agccttcgaa gtagaggctc caccagccgc ggagataggg aagttcgcag tcgtccatgc
     2941 attctcgcag agtatcgccg taccatgctt cgctcattcc tttggtacca acgaagacca
     3001 ccgcagatct gtcgcctgca gtgagccagg ctcctccggt ccattcgtcc gaatgggcgt
     3061 aaccgttcat ctggtaccat ggttcgggtt ctccctcgaa gtaatcggag tatttgatca
     3121 gaacaaccga ctggagagtc gttccgtcgg gaggtgggtt accctgattc caggggccga
     3181 tagccgccag cgtaggcccg aaaccggacc atccgccatc ccggtaccgt cccgtcgcca
     3241 gacgcattcc aggtgtgtag agatcggccc attcagaagg gatcgcgaaa aggtagtcgt
     3301 tcccgttgta cggattgatc ccttctatcc tccaggctcc ggccgggcgg ggatcatcca
     3361 gatcggtctc gcaccacata tgcgtataga agtattcgtc ctggaaatgc gcaccccagc
     3421 agagatacag tttcctggaa ctctgtgctc cctgcggctc cagtatttcg agtcccacgc
     3481 ggggcatctc ttcggcccag gcgaagagtc cgtccttgac atcggcaaac ggctggaggg
     3541 tacgggcagt gttgaggtcc gaaatgctcc tggtgggtga gatcaccggg actggtatat
     3601 ctatttccga gacaaggttc cagacatcgt ggcccgtacc gtagatggag ccgggaaagc
     3661 cgtccttctt ccctttcgga tcgcccgacg ggtcgtaggt catagacatg cctccccact
     3721 cccagctttc tgcatccggt gtcccgtcta tccactgcgg caccagaaag gcacccagat
     3781 actcgaaatc ttcgggcatt atccgtccag tggcgacggc agtgtccgta aagcacagaa
     3841 aaactgctgt aaccagtagc gtgaagagaa ctctctttaa atctttcatg ccattttccc
     3901 tccttcactt gaacgagagc ttttgacggt gaattcttca acgaaagcac gagagttcgt
     3961 caggtagttt tgattaaccg gcagcggaaa gtggaatacg accaattata gaccataaag
     4021 aaccatggag ggtggtgagt ggtggtgagt aagaaccgag agaaagagca tcgagagttc
     4081 gcttcgctca cgagaaggcg agagcgaaaa taggaactga cgatgctcca cacgcctctt
     4141 actctctctt ctctctctcc tcatctcgct ctttccgagg acggctcttc gctcttttgc
     4201 tgtataattg attcaacagc cggaacccgt cgaaccgctt ttcttgttca accatcgctt
     4261 aattgcggtg agcaactcat ttgggaggtt ttgtatgaaa agaattgttc ttactttctt
     4321 ctgtctttta ttgttggtgg gaattggctt ttcagtaatt accgcgagtg tcggaaagct
     4381 gcaggcttca aataccggta tggtcgcggc cggaaacccg tacgccgccg ctgccggtgc
     4441 cgagattctc gagaaaggtg gaaacgctat cgatgcggcg atcgccgtca gtctagcgct
     4501 gggggtcgtt gaaccctacg cttcgggaat cggtggagag ggttacgctg tcgtttcctt
     4561 caacggagaa aagatcgccg tcgacttcag gagctcggca ccgggacttg cctctattgc
     4621 caccgcggct gagcttggtt tgaaggcttc cgatttcagg accgggccgt tgagcatctg
     4681 tacgccgggt gttctggccg gcatcatcga actctggctt tatggaggct ctctcccatt
     4741 cgcagaattg atcgcacctg cgataaggct cgcgagcgaa ggctttgaag tcaactacac
     4801 attcgcctca tcgtgtggtg gtagctacga tgttctgctc gaaaacgccc ccggtttcct
     4861 gaaagaaggt cttttcgcct gggaagccgg tgacatagcc accaatcctg aactggcgga
     4921 aacctacaag tatctggcgg agaacggtcc gcaggccttc tacaatggaa cgcttgccga
     4981 caagatcgat gctctgatgc agtccagggg cggcctgctg aggaagagcg acctcgtcaa
     5041 ttacagggcg cttgtaaaaa cgccgcttca cggtacttac agagaatacg acctattcgt
     5101 tcctggcata ccggtcgggg gtccctattt gctggagact ctgaacatac tcgagcattt
     5161 caacctctcc gcgttcgcct gggacgatcc gctggtgatg aacatcatca atcaggcgct
     5221 cacgttcgcg atgatcgatt tgctctccta cagcggtgac ccggcgtatg tttacgtgcc
     5281 ggtagaaacc ctgatcagca aagaatactc caaaaccagg ttcatgaaca tcgatcttaa
     5341 ccaggctctc gacagcgccc tttactacac cgactatatc ggcaaacccc atctctttga
     5401 agatacggac tcttacgtcg aagctctgct agctgaagca ggagtcatag agttggtacc
     5461 ttctaccgcc gatgtcggcc aggatacgac gcatttcgcc gttatcgaca gaaatggaaa
     5521 cgccgttgca tggacacaga ctctgtcagg cttcttcggt tcgggcgtct accttgacgg
     5581 tttcttcata aacaacgaaa tgggaaactt caccctcgac gagacttcaa ggggcctcgt
     5641 tccgggcaag agaccggtta ccacgatctg cccgacgata atcgagaagg acggaaaagt
     5701 cgtgtacctg attggaacac cgggtggata ttacatacag tccaccataa caaatgtcat
     5761 cgttgacctg atagactttg ggatgactct cgacgaggcc cagatgacac ccaagctcat
     5821 cggatctcct aattacccgg aattcagaat ggaaacgggc ttccccgaat cctcgaggct
     5881 tttcctggag aagataatgg gccacaaggt gagagaattc cagttcccct accgctcttt
     5941 cggctctcta aacctcatcc aggtacagcc cgacgggctc atgataggaa taggagcttt
     6001 caggagagaa ggaggggcat cggcgccgga gttttaagtt tttggagagt tggagcactt
     6061 aacctgacga gagtttcggc tatgaacgga ggtactgcgc aatgaaaaaa ttacttgtaa
     6121 tactgctgat cctcatggga acgtcgcttt cgctggcggt tttcttcagg ccagatatcc
     6181 ggccgggtta cggggtcacg gaaacgaagt ggctctccta ttacttcgaa ccgctagaag
     6241 gtaccaacat ggacacggtt gtctacttca tggacagcgg gaaagccggg ccgaccttcc
     6301 ttctgatggg tggcacacac gccatggaga tcgcgggcac agtagcggcg actatattca
     6361 tagaaaacgc gatagtcgag cgcggcaggg tgatcgtaat tcccttcagc aacagcagcg
     6421 gggcttctat cgccacggtt ttctatccca atcagcctga tcactttttg caaatacagt
     6481 ctaagagcgg gccacgcttc ctcgtctatg gcgacaggaa cacagatgta acgaccaggg
     6541 ccggcccgat ccggaagtgt atctccacta tccttcgggt caagaattac ccggttacga
     6601 gtcgagaaac ctgaaccgca attacccggg acggcccgac ggaactccga ccgagaagct
     6661 cgccttcgca ataatggagc tcattaagaa ggagaacgtt gatttcaacc tggatatgca
     6721 cgagagcgat acgccggaca attacgacat cgtcaatgga gtgaaagtgc ccggcggcag
     6781 actggcctac atgctggtat gtcaccagag aggtatggag atcggggctc tggcaactat
     6841 ggagatagag ttcaactacg gagtctcgct gaaactggaa gaatcgaaca tccagttcag
     6901 gggactaagc catagagaga ttggcgatgc cactgggagc atctcttttc tggtggaatc
     6961 tcccaacccc gggttcgact tcatatggcg cgaaaaaccc gacacgctct acg
//
LOCUS       DAOOLY010000116         6986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12779_length_6986_cov_5.573366, whole genome shotgun sequence.
ACCESSION   DAOOLY010000116 DAOOLY010000000
VERSION     DAOOLY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12779_length_6986_cov_5.573366"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..404)
                     /locus_tag="PLD15_06975"
     CDS             complement(243..404)
                     /locus_tag="PLD15_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63683.1"
                     /translation="MLLTIIMIFLGLVFLGLAIKLAFFALTVIFGLAALGFVVFGVFW
                     LLTRVLSIF"
     gene            complement(427..3429)
                     /locus_tag="PLD15_06980"
     CDS             complement(427..3429)
                     /locus_tag="PLD15_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB63684.1"
                     /translation="MTENTGEYRDSMEMDFTGRNFYEEYAWECDLANGVITFNQEWQK
                     RLGYSAEAVKPGMDGWKSLIHPDDRERTIKSLMDYIEGRLEKYEAIYRIKARDGKDVW
                     VLSSGKALRTDKSGKITRVGGVQRLISDIETVELNLRERKKELEFFFAFSKLIDDCGD
                     SLEKICAGLTELLPKAFFDSEHTGVEIVYRDGVYSSKNLLKDSRFIRKPLKRDVEEIG
                     SISVYYSGYRDVRFLSEEDRVLSSIAERLGKIAIRIENSKALKESEELFKVTLASVGD
                     AVLATDAFGIVTFVNGTSCEMLGLEMQKILGRHVNSVMHIFNELTGEPAEIPVDRVLS
                     SGRKVGLANHTCLKSENGKIYFIADAASPIVMENGEIVGVVMNFRDVTSEKERERRIL
                     ESEEKFRHLIENMIGGMIILYSDDGGKTFKVRDIKSGISGLHFDRESVGGKDIGEVLP
                     AISSTSLSAAIESVWESGKPLALDPIYYDDGVLKGWWENYIYRLPSGDVVTLNYNVTE
                     RKELEEFQRLSHSNLVELSKSVASRNFDLQSFMEKAVETVGRMLRVDRAGIYMLNENS
                     EFDIRLLYEVSADKISSNAGRAMDQKLAEKYLSHIKDRRVLSIENVLEENIEDLFIRS
                     LIDVQVYGILDIPLRIKGEIIGSLYCDMRGPRSWSYEERSFAASVGDILTMALEEDRL
                     VRSEMRYRNMFEASSAMMVLIDPDSGRIVDSNPVACDFYGYGREEMRQRYTSHLGLGD
                     LEMMSDIKNGRSRRFTTRHLLAGGEIRDLEIFVGPVEIDGKMLLNCIVNDITDRVNAE
                     NKVRETMVELEHSLKDTVMLISKIVEARDPYTAGHQANVSRLAGEIAEKMGLDKKRVQ
                     WVKTAGMLHDIGKVSIPAEILSKPGKLNELEWSLIKNHPLLGREILSEVYLGGPISEI
                     VLQHHEKMNGSGYPNGLKGEEILLEARIIAVADVIEAMSSHRPYRASRGLEEAIREIV
                     MNSGELYDPAVVKACIQILESGFNLSGK"
     gene            complement(3422..5455)
                     /locus_tag="PLD15_06985"
     CDS             complement(3422..5455)
                     /locus_tag="PLD15_06985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPB63685.1"
                     /translation="MSIRSGKLMSSLEKISSLPILEGEGMDMAMEILALAGRAISADC
                     LYIYRIGNIERGHKNGFPVVKWASPSLSEIYSGLGKTCESMEEDSTILSGLEKGEIVF
                     SQSGSRVSIPVMKDRFRGAICCEDYSENRIWSDDEIAFLRIVGHSIASLFIVHSLIKR
                     DEPLEEDIEKPKQVLSAKGSENSQALKKMEYQLIVRDSVMAMINEISMLSLKAKTLEE
                     AAGIIITEVSEKPGVYRCRLSISRKRNTDIEVSSGEGFDVPFNEGQIEDRAVELSGPG
                     YLLSRHLKDGAAYRRLCISMELDSDSTGMLEVIFDGSGELDGSVSYLFGVASHFLKLA
                     LYRVKYLSEIERTRKVSQSLRRATEIITKSLRMERTIKVIVEELGKVIPFYSASLELL
                     DGDELVIVGGKWPDQLDFTGRHFTIEENSPGYDVLHRSEAILSKQAQKDYPQFNIPDF
                     HFIKSWMGVPLIIKEKPIGIIAVDSDRENAFDGLHLEALRAFADIASIGINNSRLHEE
                     VEQQAIRDYLTGIYNRRGLFELGEGELKKAKRYHTDTGLIMFDIDNFKKLNDRHGHLA
                     GDFVLKVATKICGAELRSADIFARYGGDEFVVILPMTDLAKTREAAKRLCNALCSSAV
                     EYNETRFNIRASFGVTSYRYDDTLEGMIKRADRSLYISKNAGGNRVSGGEIND"
     gene            5695..>6986
                     /gene="gatA"
                     /locus_tag="PLD15_06990"
     CDS             5695..>6986
                     /gene="gatA"
                     /locus_tag="PLD15_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPB63686.1"
                     /translation="MDINPLKLRLSEIRGLRGAFSFYRNRIERFDKDLKAFLQVLQSS
                     GEETGHSFPYALKDNILALGTRTTCSSRILENYESSYDATVTCKLKESGGHLLGKTNL
                     DEFAMGSSTENSAFFTSRNPWDLTRIPGGSSGGSAAAVAAGLTPFALGSDTGGSVRQP
                     AAMCGIVGFKPTYGLVSRYGLVAFASSLDQIGPMTRCAQDAADVMNIISGHDVKDSTT
                     MKSSFSFNIDLKSDVKGLRMAVPEEMLDYPGLEDGVKVSFMDAVEELKRAGAVVEKIS
                     IPSVKYAVATYYLIAPAEASSNLSRFDGIRFGPRHEAADYDGLVSRNRDLGFGREVKR
                     RILLGTFTLSATYYDAYYRKALRTRRLMANELKAVLTEFDFILNPSSPVVAPVIGEIS
                     DPLSYYLMDIYTIPANVAGLPSISVPIEGRPATLAGIV"
BASE COUNT         1546 a         1864 c         1683 g         1893 t
ORIGIN      
        1 aatcggtgtt acgggtgatg aaacacttgt aagaagcgat gaagaggccg gtagattccc
       61 caatttcaaa gaaatagtgc tgggttacct gcagagcaaa aaggccttcg cgccgttcga
      121 aggagagttt caacccaaat tcaaaatctg aaccggaaca tacagagaga atcatcgaac
      181 acggggcatg cgacagcgat agcattgccc cgttgtcgtt ttgtacgttg gacttttgat
      241 cctcagaaga tcgacagtac tctggtcagt aaccagaaga caccaaagac cacgaagccg
      301 agcgcggcca gtccgaagat aacagtgagg gcgaaaaagg cgagctttat cgccagaccc
      361 agaaacacga gacccaggaa tatcatgata atcgtcagca gcattttgaa cactcctttc
      421 tatttttcat ttgcccgaaa ggttgaaccc cgattcgagg atctgtatgc aagctttcac
      481 gactgcggga tcgtacagct cgccggagtt catcactatc tctcttatcg cctcctcgag
      541 tccgcgcgat gcacggtaag gcctgtgtga actcatagct tcgatcacat cggccaccgc
      601 tattatgcgg gcctccagga gtatttcctc acctttaaga ccgttcgggt aacccgatcc
      661 attcatcttt tcgtggtgtt gaaggactat ctctgagatg ggacctccta ggtaaacctc
      721 gctcagaatc tctctgccca gcagcggatg attctttatg agcgaccatt cgagttcgtt
      781 cagttttcca ggtttggaaa gtatctcggc cggtatcgag acttttccta tatcgtgcaa
      841 catcccggcc gtctttaccc actggaccct ctttttgtcc agtcccatct tttcggcgat
      901 ctcaccggcc aatctgctaa cgttcgcctg atgacctgcc gtgtaaggat cgcgagcctc
      961 cactatcttg cttataagca tcaccgtatc tttcagagaa tgctcgagct caaccatggt
     1021 ctctctgact ttattctcgg cgttgactct atcggttatg tcgtttacga tgcaattgag
     1081 cagcatcttt ccatcgatct cgaccgggcc gacgaagatt tccaggtcgc gtatctcgcc
     1141 gcctgcgagg agatgcctgg ttgtgaaacg tctggaacga ccgttcttaa tatcgctcat
     1201 catttcaagg tctccgagcc cgaggtgcga agtgtaacgc tgcctcatct cttctctgcc
     1261 atatccatag aagtcgcacg ctacggggtt ggaatcgact attctgccgc tatcgggatc
     1321 gatgagaacc atcatggcgc tgctcgcctc gaacatgttg cggtaacgca tttcgcttcg
     1381 aactagcctg tcctcttcaa gggccatcgt gagtatgtcg ccgacggaag cggcgaaaga
     1441 tctctcttcg tatgaccagc tcctgggccc tctcatgtcg cagtagagcg aaccgatgat
     1501 ctcacccttg attctcaggg gtatgtccag tatcccgtaa acctgaacgt ctattaggct
     1561 gcgtatgaag agatcttcga tgttttcctc taacacgttc tctatcgaaa gaactcttct
     1621 atctttgata tggctgagat acttttcggc cagcttttgg tccatggctc ttccggcgtt
     1681 tgaagagatt ttgtcggccg acacctcgta aagtagtctt atatcgaact cgctgttttc
     1741 gttgagcata tatatgccgg cccggtcaac ccgcagcatt ctgccgaccg tctcgacggc
     1801 cttttccatg aacgactgta agtcgaagtt tctcgatgca acggatttgg ataattcgac
     1861 caggttgctg tgcgagagtc tctggaactc ctccagctct ttcctttcag tcacgttgta
     1921 gttgagggtc accacatctc ctgaagggag ccggtagatg tagttctccc accagccttt
     1981 caatacaccg tcgtcgtaat atataggatc cagggctagg ggtttaccgc tctcccatac
     2041 gctctctata gccgccgaca gggaggtcga tgaaatggcg ggcaggactt ccccgatatc
     2101 ctttcccccg accgattctc tatcgaagtg aagaccgctt atccccgact tgatatcgcg
     2161 taccttaaaa gtctttccgc cgtcgtcgct gtacaggata atcattccac cgatcatgtt
     2221 ttcgatgaga tgtctgaact tctcttcgct ttcgagtatg cgtctttccc tctccttttc
     2281 ggaagttacg tccctgaagt tcatcacaac tccgacgatt tcgccgttct ccatgactat
     2341 cggggaggcc gcgtcggcga tgaagtagat tttcccgttt tccgatttga gacaggtatg
     2401 atttgcgagt ccgaccttcc tgccggagct gagtactcga tccacgggta tttcggctgg
     2461 ctctcccgtg agttcgttga agatatgcat aaccgaattc acgtgtcgcc ccaggatctt
     2521 ttgcatttcc agaccgagca tttcacagga cgtgccgttc acgaaagtga ctattccaaa
     2581 cgcatcggtc gccagaacgg cgtcgcccac gcttgcgaga gtgaccttga agagttcttc
     2641 actctctttc aaggccttcg agttctcgat tctaatcgct atcttcccga gtctttcagc
     2701 gatggatgaa agaaccctgt cctcttcgct aaggaaacgg acatctctgt aaccggagta
     2761 ataaacgctg atcgatccaa tttcctcgac atctctcttg agtggtttcc tgatgaacct
     2821 ggagtctttt agcaggtttt tggagctgta caccccgtcc cgatagacaa tctcaacgcc
     2881 cgtgtgttcg gaatcgaaaa aggccttcgg caagagttcg gtcagtccgg cacatatctt
     2941 ttccagtgaa tcgccgcaat cgtcgatcag tttagaaaaa gcgaagaaga attccagttc
     3001 cttctttctc tctcgcaagt tcagctcgac cgtctcaatg tccgatatca accgttgaac
     3061 accgccgacc ctcgtgatct tcccggattt gtctgtccgg agcgcttttc ccgaagacag
     3121 gacccatacg tctttgccat ctcttgcctt aattctgtaa atggcttcgt acttttccaa
     3181 tcttccctct atgtagtcca taagggattt tattgtccgc tcccgatcgt ccgggtgaat
     3241 gaggcttttc cagccatcca tacctggttt taccgcttct gcggagtatc cgagtcgctt
     3301 ttgccactcc tggttgaagg ttatgacccc gtttgccaga tcgcactccc aggcgtattc
     3361 ttcgtagaaa ttgcggcccg taaaatccat ttccatcgaa tctctatact ctccggtgtt
     3421 ttcagtcatt gatttcgcct ccactaaccc ggtttccacc ggcgttcttg gaaatgtaca
     3481 gcgatctatc ggccctcttg atcattccct caagggtatc gtcgtaccgg taagaggtca
     3541 cgccgaagct ggcgcgtata ttgaacctgg tttcattata ctccacagcg gaggagcaaa
     3601 gggcgttgca caatcttttt gccgcttctc gcgtttttgc gaggtccgtc atcggcagga
     3661 tcaccacgaa ttcatcgccg ccgtatctgg cgaagatatc ggccgatctc agttcggcgc
     3721 cacatatctt tgtagcgacc ttcaaaacga aatcgcccgc gagatgaccg tgcctgtcgt
     3781 tcagtttctt gaaattatct atatcgaaca tgatgagtcc cgtatcggtg tgatatcttt
     3841 tggccttttt tagttccccc tctcccagtt cgaagaggcc ccttctgttg tatatgcctg
     3901 tcagatagtc tcttatcgcc tgttgctcga cttcttcgtg gagcctggaa ttgttgatcc
     3961 cgatagacgc gatatcggcg aaggccctaa gcgcttcgag gtggagaccg tcgaaggcgt
     4021 tttcgcgatc gctgtcaacg gcgatgatgc cgatcggttt ttcctttatg atcaagggca
     4081 cgcccatcca tgatttgatg aagtgaaagt cggggatgtt gaattgcggg taatccttct
     4141 gtgcctgttt tgaaaggatc gcctcggacc tgtgtagcac atcgtatccg ggactgttct
     4201 cttctatcgt gaaatgcctt ccggtgaaat caagttggtc cggccactta ccaccgacta
     4261 tcaccagttc gtctccatcc agcagctcta gcgaggcact gtagaagggg atgaccttgc
     4321 cgagctcctc tacgatgact tttatcgttc tttccatcct gagactcttg gtgatgatct
     4381 ccgtggcccg gcgcagcgat tgggagacct ttcttgtcct ttcaatttcg ctgagatact
     4441 tcactctgta gagcgcgagt ttcaagaagt ggctggccac gccgaagaga tagctaacgc
     4501 tgccgtccag ttcacccgat ccgtcgaaga tcacttccag cattccggtg ctgtcgctgt
     4561 ccagctccat cgatatgcag agccttctgt aggcggcacc gtctttcaga tgtcttgaca
     4621 ggaggtagcc cggtccgctc aactctaccg ctcgatcttc tatctgtccc tcattaaagg
     4681 gaacgtcgaa accttcaccg cttgagacct cgatatcggt atttctcttc cttgaaatcg
     4741 ataggcggca tctgtaaaca ccgggttttt cggatacttc ggtgatgatt atcccggccg
     4801 cttcctccag cgttttggct ttcagggata gcatggatat ttcgtttatc atcgccataa
     4861 cgctatcacg gactatgagc tggtattcca ttttcttcaa ggcttgcgag ttttcggagc
     4921 ctttagccga aaggacctgt ttcggctttt caatgtcctc ttcgagcggc tcgtctcttt
     4981 ttatgagcga gtggacaatg aagagagaag cgattgaatg gccgactatc ctcaaaaacg
     5041 cgatttcatc gtccgaccag atcctgttct cggagtaatc ttcgcaacag atcgcaccgc
     5101 gaaaacggtc cttcataacc gggatagata cacgcgatcc cgactggctg aaaacgatct
     5161 ctcccttctc aagaccggaa aggattgtgc tgtcttcttc catgctttcg caagtctttc
     5221 ccaagccgga atatatttcc gatagcgaag gactggccca tttgactacg gggaagccat
     5281 ttttatgacc tctctcgata ttccctatcc ggtaaatgta aaggcaatcg gccgatatag
     5341 ccctgccggc aagcgccagt atctccatag ccatgtccat tccttcccct tccaggatcg
     5401 gcaacgaact tattttttcg agactggaca tcaatttgcc tgaacggata gacaccggaa
     5461 cccctccaga ctttgaaagt aactggttat ctggttatta ttttaattat aatgcaaagc
     5521 tgtgccgaat gcatggaaaa tgcagtttgc ggggtttctt atatgagata cacctctcac
     5581 tcccatcctt cgatctgtca ttacggacct ggagatgtta aaattatcta gataaaacaa
     5641 gcatgattca aaccgcataa agggtaccaa tcgcgattat aagaggtggc tgatttggat
     5701 atcaatcctt tgaaattgag actttctgag attaggggct tgcgtggcgc tttttccttt
     5761 tacaggaatc gcatagaaag gttcgataaa gatctgaagg cctttctcca ggtgttgcaa
     5821 tcctcagggg aggagaccgg gcattccttt ccctacgctc tcaaagataa tattctagcg
     5881 cttggaaccc gcactacgtg ttccagcaga attctggaga attacgaatc ttcttacgac
     5941 gcgaccgtta cttgcaaatt gaaagagagc ggcggacatt tactcggaaa gacgaatctt
     6001 gacgagttcg cgatgggctc ttcgactgag aattcggcct tcttcacttc tagaaacccc
     6061 tgggacctta ctaggatacc gggtggaagt agcggtggca gcgcggcggc cgttgcggcg
     6121 ggtttaacgc ctttcgcgct cggtagcgat acgggcggtt cggtgagaca accggcagcg
     6181 atgtgcggaa tcgtcggttt caagcccacc tatggtttgg tgtccagata cggcctcgtg
     6241 gccttcgcct cctcgctcga tcagatcggt cctatgacaa ggtgcgcgca ggacgcggcc
     6301 gatgtgatga atatcataag cggtcacgat gtgaaagatt ctacgaccat gaagagttcc
     6361 ttttccttca atatagatct gaagagtgat gtcaagggat tgaggatggc cgttccagag
     6421 gagatgctag attaccccgg tctcgaagat ggggtaaaag tctccttcat ggatgcggtg
     6481 gaggagctga aaagggctgg cgccgtggtc gagaagatct caattccttc agtcaagtac
     6541 gccgtcgcga cttactacct gatcgcaccg gccgaggcca gttcgaatct ttcgagattc
     6601 gacgggatca ggttcggacc cagacacgaa gccgccgact acgatgggct ggtgagcagg
     6661 aacagagacc tgggttttgg cagggaggtc aaacggagga tactgcttgg aaccttcacc
     6721 ctcagcgcca cttattacga cgcttactac cgtaaggcac tcagaaccag gagattgatg
     6781 gccaatgaac tgaaggccgt actgacggag ttcgatttca tattgaaccc ctcctctcca
     6841 gtggtcgcac ctgtgatcgg cgagataagc gatccccttt cgtactatct catggatata
     6901 tacacgatcc cggccaacgt tgccggcctg ccctcgatat cggtcccgat cgagggcagg
     6961 ccggcaacgt tggccgggat cgtgta
//
LOCUS       DAOOLY010000117         6953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_12880_length_6953_cov_4.236155, whole genome shotgun sequence.
ACCESSION   DAOOLY010000117 DAOOLY010000000
VERSION     DAOOLY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12880_length_6953_cov_4.236155"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PLD15_06995"
     CDS             complement(<1..1132)
                     /locus_tag="PLD15_06995"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HPB63687.1"
                     /translation="MKEIIEKYFEKNRDFMVEDIAKLVRIKSDKQEAKEGKPFGEGPA
                     EVLAVALEIASKMGFKTHNYDNYAGAIDLNDHERKLDILAHLDVVPAGNGWSITEPFE
                     PLVRDGRIYGRGTTDDKGPAVAALYAMKAVKDLNIPIKAGVRLILGTDEECGSSDIRY
                     YYGIEPEAPMTFSPDTGFPVVNIEKGGLQGRFVYELEGSNKTPRVTALRAGVKSNVVP
                     GDSIAVVEGMGPEEIERYCKAVSERTGVFFDLSTGGNTTTVKAKGVQVHAATPGKGNN
                     ALTAILELVSSIPFPESAGIEKLRALNAIFPHGDWLGKSAGVAMSDELSGDLTISLNM
                     LEYDGSSFSGTFDCRAPLCATRENLSDVIRKRLAEHRIHLENS"
     gene            complement(1148..2353)
                     /locus_tag="PLD15_07000"
     CDS             complement(1148..2353)
                     /locus_tag="PLD15_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB63688.1"
                     /translation="MGYDFETLVNRENQGNMKYIYTPGIVKKMNLISYAGAEMDFKTA
                     PVIIEALVERAKNGLMGFTLADEKYLESVRWWMRNMRGWEVQSDWIVPTYGTIHSVAT
                     TIRAFTVEGDGVIVQPPVYNRYEQAVRRTKRKIVYNHLIYRDGKYHMDFNDLEKCMKV
                     ERNKMMVLCNPHNPVARVWERSDLETIAFIAEKYGVMIFSDEIFGEITFNGHLVLPYA
                     SVAGPESRSIVSTSLGKVFNFTGVNNANMIIPEPSTREKFRTQRDADHYGSIDPMVYT
                     AVCAGYGPEGADWVVKMRSYLSENISMIREFFDRKIPAVTASEVEGSFVMWIDWRVLG
                     LGEDELYYFLLNEAYMDLDRGVNYGTEGKGFSRMNFAAPRSKIEESLGFLFDAASKRG
                     YTVSKIVNE"
     gene            complement(2329..2820)
                     /locus_tag="PLD15_07005"
     CDS             complement(2329..2820)
                     /locus_tag="PLD15_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPB63689.1"
                     /translation="MFIEKRIEELGIELPSSPLPGAMYVPVKRLGKALYISGQVPMKD
                     GKPIYTGKVGSERSIEYAQEAARLCVINMLAAVREYLGDLDRVADIVKLQAFVNSETG
                     FTNQHIVANAASQLLYDIFGEAGRHTRTAVGTNQLPMDFTVEIEAIIEIRESGEWDTI
                     SKH"
     gene            complement(2852..3469)
                     /locus_tag="PLD15_07010"
     CDS             complement(2852..3469)
                     /locus_tag="PLD15_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB63690.1"
                     /translation="MDGEEALDFLNRVARGIAEMFGSNCETLVHDMSVPKHPIVVIYN
                     GHITNRKIGSTEDVYGDLAKYRSSYLESDFVNHLAVYKSGKFIKSTTFHLKGEGYHYA
                     LGINYDFTYMREFSGMLENFTRVESDLQSAISEAGENKLSSIFNSCLEAIGKPIESLT
                     KSDRLRLISLLKREKAFAFSKSVPYVSRRLNLSRYTIYKYIKESG"
     gene            complement(3518..4867)
                     /locus_tag="PLD15_07015"
     CDS             complement(3518..4867)
                     /locus_tag="PLD15_07015"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB63691.1"
                     /translation="MSYLDRYRHALGSLPEGVYEAEVDAERRVSTLVKRSGGEIVETG
                     SSDTTELFVRASGDKTGYAYTQDLEEEPGEVLMKALGNSSLVEREQRDRLNGAFVRRA
                     SPNGIADPGILSMKKAARELEEVMRSADQYISSVTVEIRAEGFSRSVINSRGLDVEAS
                     MNLYSATATVMAEKNGLQYNTSCYTSAEDPNRLDLAGLKERIAANLGHQYDATEIESG
                     EYRVLLDATVVTNILMTAWQLFSGIKYDDGSSALSGRLGEAIGSSFFSVVDSPSHELT
                     GYRYEFDCEGSPCVKQVLVDKGRLVGLMHNITSATRLGVSSSGNAGRYALLSGSIPTD
                     IIVTPRIIHVEPGSKSVGELLEELGNGIYITDSYDVFHSINIGSGGFSIPCRGTVIKN
                     GREDRNVTGMTMNGELIGLFARVLAVGGDLYIEEFLRKSYCVGAPSLLIERLQISGK"
     gene            complement(4870..6270)
                     /locus_tag="PLD15_07020"
     CDS             complement(4870..6270)
                     /locus_tag="PLD15_07020"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB63692.1"
                     /translation="MLELEVDNLKRVIGKALSSGGDFAEIFLEDKDELNIKCSGGTIS
                     GVTTVRIKGAGIYVLYGRNSVYVYTNNLSLQSLTGCAERAGELLGALRRRSGDMDGKK
                     LSMQKTVNPNRFEIYPSSVDLREKIRVVRETDLAARNAGETIRQLNVDYFDTDQKVVI
                     FNSEGLNAEDRRITSRLRLQATVEAERLFKYEWGDYTRPQGFEAFRTKNDYTSFAREF
                     IRDMAADLKAVPVKSCNVPVVFEAGSCGTFWHETCGHQLEASAVSRNASDFAGKIGLA
                     VASEKVTLIDDGTLPGLYGSAAIDDEGFPTRKNVLIENGILRSYLCDRLCGRKLGIPS
                     TASGRRQGYMYAPVPRMSNTYLAAGNDDDEEMIRSVDRGLFVKKLGGGTGGREFSIAV
                     SEGYWIENGRISHRLRSGFILNGRGIDMIKRVDMVGRKLQNEGGSFCGADSGLCTVTS
                     FQPRMRISRMSVGGED"
     gene            complement(6432..>6953)
                     /locus_tag="PLD15_07025"
     CDS             complement(6432..>6953)
                     /locus_tag="PLD15_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63693.1"
                     /translation="FSGGQRQRIGIARALAVGPEFLVCDEAISALDVSIQAQIINLLI
                     DLQKKLDLTYLFIAHDLSIIRYISDDTIVMYLGTLVEKAETEELFLNPLHPYTIGLLE
                     AVPIADPEYEKSHKHDLLSGEVPSPINPAPGCRFASRCKFAKEICKRERPFLIYAGNG
                     HMVACHMVHSDSW"
BASE COUNT         1421 a         2001 c         1638 g         1893 t
ORIGIN      
        1 cgctgttttc aaggtgtatt ctatgttcgg ccaatctctt tctgatcacg tccgacaggt
       61 tctctctagt ggcacagagc ggtgctctgc aatcgaaggt tccgctaaaa ctggaaccgt
      121 cgtactccag catgttcagg cttatagtga gatcacccga cagctcgtcg ctcatggcga
      181 ctccggccga tttacccagc cagtctccgt gggggaagat agcgttgaga gcgcggagtt
      241 tttcgatacc cgcgctctct ggaaacggga tcgaggatac aagttcaagt atcgccgtca
      301 gtgcgttatt gccttttccc ggtgtagctg cgtggacctg tactcccttc gcctttacag
      361 tcgtagtatt gccgccggtc gaaaggtcga aaaagacgcc cgttctttcg cttaccgctt
      421 tgcagtatct ctcgatctcc tccggtccca taccctccac gactgcgata gaatcgcccg
      481 gtaccacgtt gctctttaca cctgctctga gagcggtaac ccggggcgtt ttgttcgatc
      541 cttcgagctc ataaacaaac cttccctgaa ggcctccctt ttctatgttg accaccggaa
      601 agcccgtatc gggagagaag gtcatcgggg cctctggttc gatcccgtag tagtacctta
      661 tgtctgagga gccgcactcc tcgtccgttc cgaggatgag cctaacgccg gctttaatag
      721 ggatgttcag atccttcact gccttcatcg cgtacagagc cgccacggcc ggacctttat
      781 cgtcggtcgt gccacggccg taaattctgc cgtctctgac gagcggttcg aagggttcgg
      841 tgatcgacca gccgttcccg gccggtacga catccagatg cgccagtata tcgagtttcc
      901 tttcatgatc gttcaggtct atcgccccgg cgtagttgtc gtaattatgg gtcttaaatc
      961 ccattttgga ggctatctca agcgctaccg ccagaacttc agccggccct tctccaaagg
     1021 gctttccctc cttcgcttcc tgtttgtcgc tcttgatcct taccagtttt gcgatatctt
     1081 cgaccatgaa atccctgttc ttttcaaagt acttttcgat aatctctttc atagataaat
     1141 cccacctcta ttcattaacg attttcgaga ctgtgtatcc gcgttttgag gccgcgtcga
     1201 aaagaaagcc cagagattcc tcgattttcg accttggcgc cgcaaagttc atacgggaga
     1261 agcccttccc ctcggtacca taattgacgc ccctgtccag atccatgtag gcttcgttca
     1321 gaaggaagta ataaagctcg tcctcgccga gtcctagtac gcgccagtct atccacatca
     1381 cgaaggaacc ttcgacctcc gacgctgtga cggccggaat cttcctgtcg aagaattctc
     1441 ttatcatgga tatgttttcc gacaggtagc tcctcatctt gacgacccag tctgcaccct
     1501 caggtccata cccggcgcag acggccgtat agaccatagg gtcgatgctg ccgtagtgat
     1561 cggcgtcgcg ctgtgtccta aacttttctc tggttgatgg ttccgggatt atcatgttgg
     1621 cgttgttcac gcctgtgaaa ttgaagacct ttccaagcga tgtggagact atcgatctgc
     1681 tctccggtcc ggcgacgctg gcataaggaa ggaccaggtg accgttgaag gtgatctctc
     1741 cgaagatctc gtcgctgaag atcataacgc cgtacttctc tgctatgaag gcgattgttt
     1801 caagatcgga acgctcccac accctggcaa cagggttgtg cgggttgcac aggaccatca
     1861 ttttgttcct ttccaccttc atacacttct ccaggtcatt gaaatccatg tggtacttcc
     1921 catctctgta tatcaaatga ttatagacga tttttctttt tgttcttctt acggcctgct
     1981 cgtacctgtt gtaaaccgga ggctgcacta tcacgccatc gccctccacc gtgaaggctc
     2041 tgatagttgt ggcaaccgag tgaatcgtcc cgtaagtcgg cactatccag tcgctctgca
     2101 cctcccagcc acgcatgttt cgcatccacc agcggacgct ctcgagatac ttttcgtcgg
     2161 ccagcgtgaa acccatgagg ccgttcttcg ccctttcgac gagagcttcg attatgacgg
     2221 gcgccgtctt gaagtccatc tcggcaccgg cgtagcttat gagattcatt ttcttcacta
     2281 tcccgggagt atatatatat ttcatgttac cctggttctc tctgttgact aatgtttcga
     2341 aatcgtatcc cattctccac tctctctaat ctcgattatg gcctctatct caaccgtaaa
     2401 gtccatcgga agctggttcg tgccgacggc cgttcgtgtg tgtctccctg cttcgccgaa
     2461 aatatcgtag agtaactgcg aggcggcgtt ggcaactata tgctgatttg tgaagcctgt
     2521 ttcgctgttc acgaaagcct ggagcttcac gatgtcggct accctgtcga gatctcccag
     2581 gtattccctg acggcggcaa gcatatttat gacgcacagc cttgcggcct cctgagcgta
     2641 ttctatggat ctttcacttc cgactttacc agtatagatg ggcttgccgt ctttcatggg
     2701 tacctggccc gagatataca gagcctttcc caatctcttc accggaacat acatcgcacc
     2761 gggaagcggg gacgacggca gttcgatccc cagctcctct attctcttct caataaacat
     2821 tgttttctcc ttctgagcgt atgtatctat gtcaaccgct ttcctttata tatttataga
     2881 tcgtgtatct agagaggttt agtctcctgg agacgtatgg gacggacttc gagaacgcga
     2941 aagccttctc gcgtttcaga aggcttataa gtctcagtct atcggatttc gtaaggcttt
     3001 cgatcggctt tcctatcgcc tccaggcagc tgttgaagat cgacgagagt ttgttctcac
     3061 cggcttcgct gatcgccgac tgaagatccg attcgaccct ggtgaagttc tccagcattc
     3121 ccgagaattc ccgcatatat gtgaagtcgt agtttattcc gagcgcgtag tggtacccct
     3181 cgcccttcag atggaaagtc gtcgatttta tgaattttcc ggacttatat acggccagat
     3241 ggtttacgaa atcgctttcg agatatgaag acctgtattt tgcgagatcg ccgtatacat
     3301 cttcggtcga gccgattttt ctgttggtga tgtgcccgtt gtatattacg actatcggat
     3361 gctttggaac actcatatca tgtaccagag tttcgcagtt gcttccgaac atttcggcta
     3421 ttcccctagc cactctgttt agaaagtcga gcgcctcttc tccatccata atcgactcac
     3481 tccctcttct tgtcgaatgt gtcgatgtag cttcatgtta ttttccactt atctgtagcc
     3541 tctctatcaa cagactcggt gcgcctacac agtaactctt gcgcaggaac tcctcgatgt
     3601 acagatcccc tcccacggcc agcacccgcg cgaagaggcc gatcagctca ccgttcatcg
     3661 tcatcccggt gacgttgcga tcctctctcc cgttttttat aactgttccc ctgcagggta
     3721 tggaaaaccc tcccgagccg atgtttatcg agtggaaaac gtcgtacgag tcggtaatgt
     3781 agattccgtt tcccagttct tcgagcagct ctcccacaga cttgcttcca ggttctacgt
     3841 ggatgatcct gggggttact atgatatcgg tcggaatcga gccgctgagc agggcgtatc
     3901 taccggcatt gccgctcgag gacactccca gtcttgtggc gctagtaata ttgtgcatca
     3961 atccgacgag ccttccttta tcgacaagaa cctgtttcac gcaaggactc ccctcgcagt
     4021 cgaattcgta acgataccct gtgagttcat gcgacggcga atcgactacc gaaaagaagg
     4081 acgatccgat cgcttctccc agtcttcccg ataatgccga ggagccatcg tcgtatttga
     4141 tcccgctgaa gagctgccag gccgtcatca atatgttcgt gactacggtg gcgtctagca
     4201 gaactctata ctcgccagac tcgatttcgg tcgcgtcgta ctggtggccg aggttggcgg
     4261 caatcctttc ctttaatccc gcgagatcga gacggttcgg atcctctgcg ctcgtgtaac
     4321 aggaggtgtt gtactgcagg ccgttctttt cggccataac cgtcgccgtc gcactgtaga
     4381 gattcatgga cgcttcgacg tcgagtccgc gggagttaat cacgcttctg gaaaaacctt
     4441 cggccctgat ttcgaccgta acgctcgata tatattgatc ggcgctcctc atgacctctt
     4501 ccagctctct tgcggctttc ttcatcgaca gtatgccagg gtcggctatt ccgttaggtg
     4561 acgcccttct cacgaaggct ccattgagtc tgtctctctg ttctcgctcc accagactgg
     4621 aatttccaag cgctttcatg aggacctcgc ctggttcttc ttccagatcc tgcgtgtagg
     4681 cgtacccggt cttgtcacct gaagcccgta cgaagagctc tgtggtgtcg gacgagccgg
     4741 tctctacgat ttccccgccg gaacgcttga ccagtgtgct gaccctccgt tcggcatcta
     4801 cctcagcctc gtacacccct tctggaaggc ttcccaaagc gtgacgatat ctatccaggt
     4861 aactcatgat caatcctccc cgccgactga catacgcgat atcctcattc tgggctggaa
     4921 actggtgaca gtacacaacc ccgaatcggc tccgcagaaa ctgccgcctt cgttttgaag
     4981 ctttctcccg accatatcga ccctctttat catgtctatg cccctcccgt tgaggatgaa
     5041 accgctcctg agtctgtggg agatcctgcc attctctatc cagtaacctt cactgactgc
     5101 aatcgagaac tccctgccac ccgtgccgcc tccaagcttc ttcacgaaga gacccctatc
     5161 aacagaacga atcatctctt catcgtcgtc gttgccggcg gccagataag tgttgctcat
     5221 gcggggtacc ggagcgtaca tgtatccctg cctcctcccg cttgccgtag aagggattcc
     5281 cagttttcta ccgcacagcc tgtcgcacag gtagctcctc agaattccgt tttcaatgag
     5341 gacgttcttg cgtgtcggga agccctcgtc gtcgatggca gccgaaccgt aaagacctgg
     5401 aagcgttccg tcatcgatca gagtgacttt ttcagaagcg acggctagac ctattttacc
     5461 ggcgaagtcg ctcgcgtttc tcgagacggc cgaggcctcc agctggtggc cgcaggtctc
     5521 gtgccagaat gtaccgcaag agccggcctc gaagacgacc ggcacattgc acgattttac
     5581 cggcacggcc tttagatctg cggccatatc tcttatgaat tccctggcga aggaggtata
     5641 atcgtttttt gtcctgaagg cttcgaagcc ctgcggacgt gtgtaatcac cccattcgta
     5701 cttgaaaagc ctttcggcct ctactgtagc ctggagtctc agtctgctcg ttattcgcct
     5761 gtcttcggca tttagtccct cgctgttgaa tatcacgacc ttctgatctg tgtcgaaata
     5821 atcgacattg agctgcctga tggtttctcc ggcgtttctg gcggcaagat ccgtctcgcg
     5881 cacgaccctg atcttctccc tgaggtccac cgaagaggga tagatctcga atctgttcgg
     5941 atttacggtc ttttgcatag aaagcttttt gccgtccata tcgccagatc gccttcttaa
     6001 agcgcccaaa agctcgcctg ccctttcggc gcaacctgtg aggctctgca aactaagatt
     6061 gttggtatat acgtacacac tgttgcggcc atacaacacg tatattccgg cccctttgat
     6121 ccgcaccgta gtgactccgc ttatcgtccc accagagcat tttatattca attcgtcctt
     6181 atcctccagg aagatctcgg cgaagtcacc tcccgaggac agagcctttc ctataactct
     6241 cttcagatta tctacttcaa gttcaagcaa agcaaagcct cctttttaat cgtaattgag
     6301 aagggctgtc ttccaatgag agcgtcaggc gtcgttcttg gaaagaccga gaagaacaat
     6361 tctccgtcca ccgtcctccg agaatagcgg aaggagccgt ccttggttct tccaccactt
     6421 ctcctactcc ctcaccagct atcgctgtgg accatgtggc aggctaccat gtggccgttg
     6481 cctgcgtata ttaagaatgg cctctccctt ttgcagattt ccttcgcgaa cttgcatcgc
     6541 gatgcgaaac gacagcctgg ggcggggttt atcggactgg gaacctctcc cgaaagcaga
     6601 tcgtgtttgt ggctcttctc gtattcggga tcggctatcg gaacggcctc aagcaatccg
     6661 atcgtatagg ggtgcagagg attcaaaaag agttcttcgg tctcggcctt ctcaacaagt
     6721 gtacccagat acataacgat tgtatcgtcg gagatgtatc tgattatact taaatcgtga
     6781 gctatgaaca ggtacgtcaa atccagcttc ttctggagat ctatcagaag atttatgatc
     6841 tgggcctgta tcgaaacgtc aagcgccgaa atcgcctcgt cgcataccag gaactccggc
     6901 cctaccgcca gtgcccgcgc gatcccgatg cgctggcgct gcccgccgct gaa
//
LOCUS       DAOOLY010000118         6871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13109_length_6871_cov_5.659331, whole genome shotgun sequence.
ACCESSION   DAOOLY010000118 DAOOLY010000000
VERSION     DAOOLY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13109_length_6871_cov_5.659331"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..2443)
                     /locus_tag="PLD15_07030"
     CDS             complement(224..2443)
                     /locus_tag="PLD15_07030"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPB63694.1"
                     /translation="MKRALVVIVLVTLIPLIVFSSLIHTAEDGVIRGTIKSYRDGVLT
                     FQLAAARVDVDLDIAKVLKISFDEKTIRGQGLVMKNGLNIPYTILVSIEDGKAIFALP
                     FGMMYVDSLEKLAFLNTQDFTELSLPEEKDFLVETVTEAKFEAALEKAEGDGYHFASP
                     YGTLIVPASHITKMRIGQGPYSESGTTLPGNVVIDGAIHSFDGKYHTLKTDFGEIKVL
                     AGALNSWVNEAPGDPIEAESFLIFKNLQQVTGSVRNWSSGTVEYATPWGALQIPQNEI
                     AQIIMGKSIGLQITTTPSGADVSFDGEPTGKSPVLISTLTEGKHTLAVSMPGYRTVER
                     EVYTTPMMYSQIEVVLEYGNFWETKTPMPTARSSHGAVVCDGRIYAIGGFDGNSRLNR
                     VEAYDPATDGWTEVAPMPTARSSIAAVLCQDRIYVIGGHDGSKRLSKVEAYDPASNAW
                     TTVSPMETARNSLTAVAYEERIYVIGGFDGSKSQSRVDVYDPRTDKWSSAAPMPTARY
                     GLAAVVYGGRIYAMGGNDGSVRLDRVEVYDPATDGWTEVAPMPTAREWLTAVVYGERI
                     YAIGGYSGSVSNRVEVYDPANDSWIEAVPMSAGREWFAAVVSEDWIYAVGGSDGKNHL
                     NRVEAYNPAGIPPVASSTEVADDLADRAEFDLRLVVVAPITADFYLGESHDLEIELVG
                     PTNFKESFENVASGDPNTVTIASLKAGTYEIRTKWGDASRTWTVELNDGESTVRFMM"
     gene            complement(2694..3350)
                     /locus_tag="PLD15_07035"
     CDS             complement(2694..3350)
                     /locus_tag="PLD15_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPB63695.1"
                     /translation="MIREKLVEYAKLAWDRKLTESTGGNMSVRHGSEILITPTTVIKH
                     FLVPEDIVAMTPEGKQTGGLRKASSEYRMHLRIYEKCPDVGSVFHAHPAHATAMAVMQ
                     RAIPVNTLPEAALMLAPITYLPYKMPGTDEFADAFNEGLEMGSRVFVLQNHGVTAAGK
                     DIEEAYAKLETLEFLAQIAFLSGAKPGHLEIPTEDILLFLEHVLGRKVTDYRSDYWFK
                     "
     gene            complement(3377..4783)
                     /locus_tag="PLD15_07040"
     CDS             complement(3377..4783)
                     /locus_tag="PLD15_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB63696.1"
                     /translation="MKYNSLNLELLKELQSMLTVPIKYDEETLERYSKDETAELKAVK
                     PEVVVFPVSTEEVSGVMKFANRYSIPVTPRGAGTGLSGGAVPAFHGIVMSLEKMNRIL
                     DFDAANMMITVEPGVITGEIQKLADRHGLVYGGDPCSSESSSIGGNIAENAGGNKVMK
                     YGPTGYHVYGLEVVLPSGEVTRLGGKMIKDVSGLDLIHLIVGSEGTLGIATGITLRLL
                     PKPKYSVALLIPFPDIDTAIAAVPKLMTGSGVVPTSLEFMDASSIKAVCSFLNMEFPY
                     ADAGAHLIVEVEGSGRDSVFDDYVKLGESAIEAGGLEAFVADNRNTREKLWKARKSIA
                     EALASISPVHCMEDVSVPMASIPELIKFSEEIARDRGLEMIAFGHAGDGNVHVSFIKG
                     DMPEERWNEALPLALKELYAKTTALGGCISGEHGIGLKRKKYLASITDKAQLDLIRGI
                     KRAFDPSYILNPGKIVDL"
     gene            complement(4780..5778)
                     /locus_tag="PLD15_07045"
     CDS             complement(4780..5778)
                     /locus_tag="PLD15_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPB63697.1"
                     /translation="MSEIWVIAEKRGTQIHSSTYELLGKARELAQKNGSSVALALLIQ
                     ERPTGDTFRSLFRGGADRVILAVDKSFERFDFGPYIRVLKEMVLQRPPEVILAPATSS
                     GRTYMPGLAALLGTGLTADCTGLDIEEGTGNLLQTRPAIGGNIMATIKTPKHRPQMAT
                     VRPKIFSPLGEVYSEHGEIVDFPVRPELVESPARLLKFDSIGEGEKGVQDAEIIIAGG
                     MGLRRMENMAPIYKLARMINGTVGASRKIVDSKWIGHEAQVGLSGHTVKPKIYIAAGI
                     SGAVQHIAGMQTSEFIVAINRDRNAAIFNFADIGLVGDATEILTRLVEAMEMEVRE"
     gene            complement(5771..6163)
                     /locus_tag="PLD15_07050"
     CDS             complement(5771..6163)
                     /locus_tag="PLD15_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63698.1"
                     /translation="MDIPAITYVSRIIEVGEDGMVAEREVEDGRETWKVPYPVLLSVT
                     KNVNEPRLPTLKGKKKARNANIEKMGSEALGLEPSTIGLTGSPTRVVKINTPKLSRQA
                     RLYEGSELVEGIDRLKALLKPYLEVNHE"
     gene            complement(6163..6564)
                     /locus_tag="PLD15_07055"
     CDS             complement(6163..6564)
                     /locus_tag="PLD15_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63699.1"
                     /translation="MRILVLLKQVPDTDEVKLDPENGTMIREGVGTVINPLDLHALES
                     AIRIKESGEHELTVVSMGPPAAEEALREAIAMGADRAVLLTDRKFAGADTWATAKTLS
                     GMIELEGPYDLILAGEKATDGEDRLGRKRRP"
     gene            complement(6616..>6871)
                     /locus_tag="PLD15_07060"
     CDS             complement(6616..>6871)
                     /locus_tag="PLD15_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63700.1"
                     /translation="NCNTGFFGRILEGYGLKPKISMTGDSFGVFPVVSVGDRGERLVY
                     VFNEDNFERRIRLRFEEEPLFGGEELFLRAREVAVEMNYP"
BASE COUNT         1220 a         2076 c         1844 g         1731 t
ORIGIN      
        1 ttctcccatc gccaatgtta tccgacagtt tctcgttagc agtactgctc ctacctccca
       61 catttatata taatcgatta tactacctga gcttcgacag ccgtccccgg cctttctcag
      121 gatggcaggt ccatctctgg cagaaaaaat cccgttcgtg aattccgaac gggattttag
      181 ggcagattac acgggataat ttcgaaaggt taagagttca ctcttacatc atgaatctca
      241 cggtcgattc accgtcgttc agttcgacgg tccaggttct gctcgcgtct ccccacttcg
      301 tccttatttc gtacgttccg gccttcaggg aggcgatcgt cacggtgttc ggatcgcctg
      361 aggcaacatt ttcaaaggat tctttgaagt tagtcggtcc aaccagttct atttccagat
      421 cgtggctctc gcccaggtag aagtcggccg ttataggggc gaccacaacg agtcggagat
      481 cgaactcggc ccgatccgca agatcgtcgg cgacctctgt cgatgaggcg acgggaggta
      541 ttccggcggg gttgtacgct tcgaccctgt tcaaatggtt ctttccgtcc gatccgccaa
      601 ccgcgtagat ccaatcctca ctgaccaccg cagcgaacca ctcccttccg gccgacatcg
      661 gcacggcctc gatccagctg tcgttagcgg ggtcgtaaac ctcgactctg ttagatacgc
      721 ttccggagta cccgcctatg gcgtagatcc tctcgccata aactacggcc gtcaaccact
      781 ctctggccgt cggcatcggc gcgacttctg tccagccgtc ggtggcggga tcgtaaactt
      841 cgaccctgtc gagccggacg cttccgtcgt taccgcccat ggcgtatatc ctgccgccgt
      901 aaaccaccgc ggccagtccg tacctcgcgg tgggcatcgg tgcggccgaa ctccacttgt
      961 cggtgcgggg atcgtacaca tctactctgc tctggctctt gctgccgtcg aacccgccta
     1021 tcacgtagat cctctcttcg taagccaccg ccgtgaggct gtttcgggca gtctccatag
     1081 gcgaaactgt cgtccatgca ttcgaggcgg gatcgtatgc ttcgactttg ctgaggcgtt
     1141 tgcttccgtc gtggccgcct atcacgtaga ttctatcctg gcagagaacg gccgctatac
     1201 tgctcctggc agtcggcatc ggcgcgactt cggtccagcc gtcggtagcg ggatcgtacg
     1261 cttcgactct gttgaggcgg ctgtttccgt cgaagccgcc tatcgcgtag atccttccat
     1321 cacagaccac cgcgccgtgg ctgctcctgg ccgtcggcat cggtgtttta gtctcccaga
     1381 agttcccata ctccaggacg acctctatct gcgagtacat cataggagtg gtgtaaacct
     1441 cgcgttcgac cgtccggtag ccgggcatgg aaacggccag ggtgtgcttc ccctcggtga
     1501 gcgtggagat caggacggga ctctttcctg tcggttctcc gtcgaaggag acgtcggccc
     1561 cggagggcgt cgtcgttatc tgcagaccta tcgactttcc catgattatc tgcgctattt
     1621 cgttctgcgg tatctgcagt gctccccagg gagtcgcgta ctccaccgtt ccactcgacc
     1681 agttccgtac cgagccggtg acctgctgga ggttcttgaa gatcaggaaa gactcggcct
     1741 ctatcggatc gccgggcgct tcgttgaccc atgagttcag ggctccggcg agaaccttga
     1801 tctctccgaa gtccgtcttc aacgtgtgat acttgccgtc gaagctgtga attgcgccgt
     1861 ctatcacgac gtttcccgga agagttgttc cagactcact gtaaggaccc tgcccgatcc
     1921 tcatctttgt tatgtgggag gccggcacga tcagtgtacc gtagggagag gcgaagtgat
     1981 acccgtctcc ctccgctttt tcgagggccg cttcgaactt cgcctccgta accgtttcga
     2041 ccaggaagtc cttttcctcc ggtagcgaaa gttccgtgaa gtcctgggtg ttgaggaagg
     2101 ccagtttttc aagggaatcg acgtacatca tgccgaaggg aagggcgaag atcgccttgc
     2161 catcctctat ggagacgagg atggtgtaag gtatgttcag gccgttcttc atcaccaggc
     2221 cctgccccct gatggtcttc tcgtcgaagg agatcttcag gaccttcgct atgtccagat
     2281 ctacatctac tctggcggcg gccagctgga aggtgagaac cccgtcccga taagatttta
     2341 tcgtgcccct tattaccccg tcttcggccg tgtggataag tgaagagaac actatcagcg
     2401 gtatcagagt tacgagaacg ataacaacga gcgcccgttt catctcaaac accccctatt
     2461 cagtaagtgc ctcttgctga atccacctcg attatattcg ctttgtgtcg ctcgtccaag
     2521 ttgaagttga agacacgacc gggcggagtt gttcgccgat aacgggccgc aactgtcgca
     2581 ctccgataac cggaccggaa agccggggat agtcgagttt gaactgaacg gcacgtgtcc
     2641 ggcccgcctt gagcctccag atccggagag ctttttggga ttctatgaag taattatttg
     2701 aaccagtaat cgctcctgta gtcggtgacc ttccggccga gcacgtgctc cagaaagagc
     2761 agtatgtctt ctgtcggtat ttcgagatga cccggtttcg ccccggaaag gaaggcgatc
     2821 tgcgcgagaa actcgagcgt ctccagtttt gcgtacgcct cttcgatatc ctttccggcc
     2881 gccgtcacgc cgtggttctg aagtacaaag acacgcgaac ccatttccag accttcgttg
     2941 aaggcatcgg cgaattcgtc cgttcccggc attttgtaag gcaggtaggt tattggagcc
     3001 agcatcaggg ccgcctcggg cagagtgttg accgggatcg cccgttgcat aacggccatg
     3061 gctgtggcgt gggcaggatg ggcgtggaag accgacccca catcgggaca tttttcgtag
     3121 atcctcaggt gcatccggta ttccgaagag gccttccgca agcccccggt ctgtttccct
     3181 tcgggcgtca tcgcgactat atcttccggc accagaaagt gttttatcac cgttgttggg
     3241 gtaatcagga tctcgctccc gtgtcgtacg ctcatattgc ctccggtgct ttcggtgagc
     3301 ttccggtccc aggccagttt agcgtactcg accagtttct ctcttatcat cgtttgcctc
     3361 caggttttta tttattctat agatcgacga tcttgccggg gttgagtatg tacgacgggt
     3421 cgaaggctct cttgataccc cgtatcaggt cgagctgggc tttgtctgtt atcgaggcca
     3481 ggtacttctt cctcttcagg cctatcccgt gttcgccgct tatgcagccc ccgagcgcgg
     3541 tggttttggc gtaaagctcc ttgagcgcca gcgggagggc ttcgttccat ctctcctctg
     3601 gcatgtcgcc ctttatgaag gatacgtgga cgttaccgtc gcctgcatgg ccgaaggcga
     3661 tcatctccag acccctatcc cgggcgatct cttcggagaa ctttataagc tcgggtatgc
     3721 tggccatcgg aaccgatacg tcttccatgc aatgaaccgg gctgatcgag gccagcgcct
     3781 cggctatcga tttgcgggcc ttccagagct tttcgcgcgt gttcctgttg tcggcgacga
     3841 aggcctccag ccctccggcc tctatggcgc tctctccgag tttgacgtag tcgtcgaaga
     3901 ccgagtctct gccgctccct tcgacctcga ctatcaggtg ggctccggcg tcggcgtagg
     3961 ggaactccat gttgaggaag ctgcagacgg cctttatcga tgaggcgtcc atgaactcca
     4021 gcgaggtcgg taccactccc gaaccggtca tgagcttcgg aacggccgcg atcgcggtgt
     4081 ctatgtcggg gaaaggtatc aacagggcca ctgaatattt gggtttgggc aggagcctca
     4141 gcgttatccc ggtagctatg ccgagcgttc cctccgatcc gactatcaga tgtatcagat
     4201 cgaggccgct gacgtctttg atcatctttc cgcccagacg ggttacctca cctgacggga
     4261 ggacgacctc cagtccgtaa acgtggtagc cggtcggccc gtacttcatg actttgtttc
     4321 cgccggcgtt ttcggcgatg tttccgccga tcgacgagct ctcgctgcta caggggtctc
     4381 ccccgtacac cagtccgtgt ctgtcggcca gtttctgtat ctcgccggtt atgacgcccg
     4441 gctcgaccgt tatcatcatg ttggccgcgt cgaaatcgag tatcctgttc atcttctcca
     4501 ggctcatcac tatgccgtgg aaggccggga cggcaccgcc ggaaagccct gtgccggcgc
     4561 ccctgggggt taccggtatc gagtatctgt tggcgaattt catcacgccg gagacttcct
     4621 cggtactcac ggggaagacg accacttcgg gcttcacggc cttcagttcg gccgtctcgt
     4681 ccttcgaata tctttcaagc gtctcttcat cgtatttgat cggcaccgtg agcatggatt
     4741 gaagctcctt caaaagctca aggttcagag agttgtactt cattcgcgca cctccatttc
     4801 catagcctcg accagccggg tcagtatctc ggtggcgtct ccgacgagtc ctatatcggc
     4861 gaagttgaag atcgcggcgt tcctgtccct gtttatggcc acgatgaact cggaggtctg
     4921 cattccggcg atgtgctgca ccgccccgga gataccggcc gctatgtata tcttcggctt
     4981 caccgtgtgt ccgctcaaac ctacctgggc ttcgtggcct atccatttgg aatcgacgat
     5041 ctttctggaa gcgcctacgg tgccgtttat cattctggcc agtttgtata tgggcgccat
     5101 gttttccatc cttctcagcc ccattccgcc ggcgattatt atctcggcgt cctggacgcc
     5161 cttctcgccc tctccgatcg agtcgaactt caggagtctg gccgggcttt cgacgagttc
     5221 cggtctcacc ggaaagtcaa ctatctctcc gtgctcgctg taaacctcac cgagcgggga
     5281 gaagattttc ggtctcacgg tcgccatctg tggcctgtgc tttggagtct ttatggttgc
     5341 cattatgttg ccgcctatcg ccggtctggt ctggagcagg ttgcccgttc cctcttctat
     5401 gtccagcccc gtacagtcgg cggtgaggcc cgtgccgaga agcgccgcta aaccgggcat
     5461 gtacgtgcgg cccgagctgg tggccggggc cagaatcact tctggcggtc tttgcaggac
     5521 catctctttc aaaaccctta tgtacggccc gaagtcgaac ctctcgaagc ttttgtcgac
     5581 ggcgagtatc accctgtcgg cccctcccct gaagaggctc ctgaaggtgt ccccggtcgg
     5641 cctttcctgt atcagaagcg cgagcgctac ggacgaaccg ttcttctgcg ccagctccct
     5701 ggcctttcca aggagttcgt aggtgctgga atgtatctga gtaccccttt tctcggcgat
     5761 cacccatatc tcactcatgg ttcacctcca ggtaaggctt caggagagct ttgagcctgt
     5821 cgatcccctc gaccagttcg cttccttcgt aaagcctcgc ctggcgcgag agtttgggag
     5881 tgtttatctt gacgaccctt gtgggcgaac ccgtgaggcc gatcgtcgag ggctccagtc
     5941 ccagagcctc gctgcccatt ttctctatat tcgcgtttct ggccttcttt tttcctttca
     6001 gcgtggggag gcgcggttcg ttgacgtttt tggtcaccga cagaagcacg gggtaaggca
     6061 ccttccaggt ttctctgccg tcttccactt ctctctccgc caccattccg tcctcgccga
     6121 cttcgattat cctggaaacg taggtgatgg cagggatatc catcatggcc gccgcttccg
     6181 gcccaacctg tcctccccgt cggtggcctt ttcgccggcg agtatcagat cgtacggccc
     6241 ttccagctct atcatcccgg agagagtctt cgcggtagcc caggtatcgg cgcccgcgaa
     6301 ctttctgtcc gtcagcagca cggccctgtc ggcccccatg gctatcgcct cgcgcagcgc
     6361 ctcctcggcg gcgggaggcc ccatggaaac gacggtcagt tcgtgctccc cgctctcttt
     6421 gatccttatg gcgctctcga gcgcgtgcag gtcgagtgga tttataaccg tcccgacccc
     6481 ctctcttatc atcgttccgt tctcagggtc gagttttact tcgtcggtgt cgggcacctg
     6541 tttaagaagt actagtatcc tcaacgctac tcccccttca aaattagatt catatgtcta
     6601 tatattaata cagcttcagg ggtaattcat ctccacagcc acctctctcg cccggagaaa
     6661 gagttcttcc cccccgaaaa gcggctcctc ttcgaagcgg agtcttatac gcctttcgaa
     6721 gttgtcttcg ttgaagacgt aaacgagcct ctctccgcgg tcgccgacgc tcacgaccgg
     6781 gaagaccccg aaggaatcgc ccgtcatgga gatcttcggt ttcaatccgt agccctcgag
     6841 aattcggccg aagaagccgg tgttgcagtt g
//
LOCUS       DAOOLY010000119         6817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13240_length_6817_cov_4.339397, whole genome shotgun sequence.
ACCESSION   DAOOLY010000119 DAOOLY010000000
VERSION     DAOOLY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13240_length_6817_cov_4.339397"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..802
                     /locus_tag="PLD15_07065"
     CDS             167..802
                     /locus_tag="PLD15_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63701.1"
                     /translation="MKRYLSVMAFFALSCTLIFAHIPTFLKAEDFSGGSYFVEDIDLS
                     QIFYYEFSGEETIVFSFEGLPGKNLHILFGVPVGVPVMETTYSFRPGLAIYRPDGQLK
                     DGFDFTQTEPEVMYEFFGDTNSYKHLGYDSLMESEGTYRIEISSREAGRAWITFGLRE
                     NFTFKQILLLPEWIRQIREFHYMKGLARWEIYGLVGLGVLTAGVIALIVFL"
     gene            1190..2284
                     /locus_tag="PLD15_07070"
     CDS             1190..2284
                     /locus_tag="PLD15_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB63702.1"
                     /translation="MQKPKYLTKVDLIEELSEDQIGQMNEVIEQFPFRANDYYLSLID
                     WDDPNDPIKRIIMPDIEELDEWGEMDASNEHLYSVVPGMEHKYEDTALLLVSRVCGSF
                     CRFCFRKRLFSTENREVTNDVGPGIEYIRKHREITNVLLTGGDSLILSTDKLSDIVGR
                     LREIEHVGIIRFGSKMVAFNPYRIINDPALPEMIRKYSTSEKRIYIMAHFNHPAELTD
                     VAVEGLNILRNAGAVICNQTPMIRGVNDDADTMAELFRKLSFAGIPPYYVFQCRPTRG
                     NHTYAVPAEEGYTVFKKAIDRVSGLAKRARFVMSHATGKIEYVGIDENKIYMKYHRAA
                     DPRRYDLFMAFDRNPDAYWLDDYVDPDSLV"
     gene            complement(2308..3045)
                     /locus_tag="PLD15_07075"
     CDS             complement(2308..3045)
                     /locus_tag="PLD15_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPB63703.1"
                     /translation="MNPRVTEERYRVKYYELTRNWKASVASIMDYFNDVVTLQSVEVG
                     HDVETMRFSGYAWLLLRWEIDIQRSPAFMENLNVRTVPYSMNKFYAYRRFEVIDDSGA
                     LIVHGKSQWILIDASRRRPVRIADRHYEVYGLSKDFNEPLKFEDPPEPGKSDLSTVLK
                     VRESDLDTNGHANNVSYVRWIMQTVPGDFESKELKKLCIQYKHESMKGEEVLVESGFE
                     DLDGLKIGLHRITSSGKKLAIACTKWL"
     gene            3155..3496
                     /locus_tag="PLD15_07080"
     CDS             3155..3496
                     /locus_tag="PLD15_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPB63704.1"
                     /translation="MLAYCGIDCSNCPVYRATMTNDEILKRETADKWGREFGFVLEKS
                     DMNCTGCRSEILFKLCFDCPFKDCCSKKGIDNCGQCAEYPCQTLERFLRPIPEARASL
                     DEIHRNFHGGT"
     gene            3499..4401
                     /locus_tag="PLD15_07085"
     CDS             3499..4401
                     /locus_tag="PLD15_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPB63705.1"
                     /translation="MKKTFTLLILQVIFAAMILALTLVPVKVETASGPKDLTYATEFF
                     DLWNSVQDYTHAKVINVVDGDTLLVSIDGVETTLRLIGIDTPETVHPTKEVEFFGIEA
                     SNLAKGVLSDKEIIISYDWNPTDVYGRTLAYVWLEVEYYGFKQYIMYNLLGVINGYGR
                     AYLAFAFDEKLMDLFAQAQDIPRTLSLGLWNEPEDEEEEIYYDGPVQIAYILADSLDE
                     YVMIYNIGDKPVNLKGWQILAVTNGQKYTFDDLILEPGMAVHIHSGPEASVNVWKKSY
                     IWRNKKAEGRLFDSSGNLVSVYSY"
     gene            4651..5676
                     /gene="ltaE"
                     /locus_tag="PLD15_07090"
     CDS             4651..5676
                     /gene="ltaE"
                     /locus_tag="PLD15_07090"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HPB63706.1"
                     /translation="MKWIDLRSDTVTLPGEEMRAAMANAEVGDDVYGDDPTVNRLEEI
                     AAEVLGKESAVFVPSGTFGNQLSILTHTLRGDEVIIPASNHIIVHEAGASAVIAGVQM
                     RTLDCDDGMPSVDRILKAIRGEDLHYPRTGLICLENAHSSGRVLPMEYMKEVYSLARE
                     RAVPVHLDGARIFNAAVSLGIDAGEIASKADSVMFCLSKGLGAPIGSMLVGTKEFISR
                     ARKGRKIMGGAMRQAGIIAAAGIMAIEKMIDRLGEDHENARYLAKKLEEIKGIEIMRD
                     RLDINMVFFKLDQGRPEAIVGELYREGIKINPPEGDERRLVTNLDVSRSDLDTFIARF
                     TKAVKSC"
     gene            6240..>6817
                     /gene="cas6"
                     /locus_tag="PLD15_07095"
     CDS             6240..>6817
                     /gene="cas6"
                     /locus_tag="PLD15_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HPB63707.1"
                     /translation="MSLIKESLKAFSAEEFERIYGKRAGRNIVFNCYFGEDMQIDKEN
                     ENIILKSPSFLLEVSSPDTKLIAMIYNGLRKNREYTVFGSRIRLKEIKPVNTLIRTKD
                     KIRVRSVCPILVKSSPDSGSRYLLPGEESFYGSLNHFCKVRYEEFFSEEFKGSVELLP
                     LELKRVVVKHMGTYHTGFKGIFELSSSSKMLQ"
BASE COUNT         1830 a         1638 c         1702 g         1647 t
ORIGIN      
        1 gcctacggtg ctgatcctgc tggaaggccg ggaattgaac agatggagca ggtatttttc
       61 gatcgaggag atcgtgagat acctcgagag gatagacagt ctccggtgat tatcatcccc
      121 gatctgataa aataaattat agttgttcta aacaaggagg aactatatga agcgatattt
      181 atcagttatg gctttcttcg cactgtcatg cacgttgatc ttcgctcaca ttcccacatt
      241 cttgaaagcg gaggatttct caggcggctc atatttcgtg gaagatatcg atctctcgca
      301 gatcttctat tacgagttct caggagagga gacgatcgtc ttctcgttcg aaggcctgcc
      361 aggcaagaat ctacacatcc ttttcggcgt accggtggga gtacccgtca tggagaccac
      421 ctactctttc aggccgggac tggccatata caggcccgac ggccagctga aagacggctt
      481 cgatttcacg cagaccgagc cggaggtcat gtacgagttc ttcggagaca ccaattccta
      541 caagcacctc ggctacgaca gcctaatgga atcggaggga acttacagga tcgagatctc
      601 ctcgagagag gctgggaggg cctggataac cttcgggttg agggaaaact tcacgttcaa
      661 acagatcttg ctgctgcccg agtggataag acagataagg gagttccact atatgaaggg
      721 actggcgagg tgggagatat acggtcttgt cggactgggg gttctcacgg cgggagtgat
      781 agcgctcatt gtgttcctct gaaagcgaaa gcggcgtaaa gcggaaacgg ccgatagata
      841 atagcaaaga gacgtccttt tcgacgtctc tttttatatt ttatcacgga taatctttaa
      901 agtcaaccgg tctggcgatt aaccgcgtgt aacttatgcc tctggaagcg cctcgaagcg
      961 ccgcatttta atacactgcc aatacctgtg cggggtacac atccaggagg ctcataagat
     1021 ataatatata gtgaatggtg ttgaaaattc cgtatcgtaa ttctttcgta gtcatctgat
     1081 aggaccggct gttcgaacaa ttgaacagtt atatccagcg agtgcagaga tacacggagt
     1141 tttttcatgc tcaacaatat cgaatcaaac agaataggga ggtagtaatt tgcagaaacc
     1201 aaaatatctt acaaaggtag atctgatcga agagcttagc gaagatcaga tcggacagat
     1261 gaacgaagtg atagagcagt tccctttcag ggccaacgat tattacctga gcctcataga
     1321 ctgggacgat ccgaacgatc ccataaagag gatcataatg cccgatatcg aagagctcga
     1381 cgagtggggg gagatggacg cctcgaacga gcatctctac agtgtcgtgc cgggcatgga
     1441 acacaagtac gaagacaccg ccctgctcct ggtctccaga gtttgtggca gcttctgcag
     1501 gttctgcttc aggaagaggc tcttctcgac ggagaacagg gaagttacaa acgacgtcgg
     1561 accggggatc gagtacataa gaaagcacag agagataacc aacgtactcc tcaccggcgg
     1621 agattccctg atcctctcga ccgacaaatt atccgatatt gtcgggcggc ttcgcgagat
     1681 cgaacacgtt ggaatcataa ggttcgggag caagatggtg gccttcaacc cttacaggat
     1741 aatcaacgat cccgcgctgc ccgagatgat tagaaagtac agcaccagcg aaaagaggat
     1801 ctatatcatg gcgcacttca atcatcccgc agaactgacg gacgttgccg tggaggggtt
     1861 gaacattctg aggaacgccg gcgcggtcat atgcaaccag acgcctatga taaggggcgt
     1921 caacgacgac gccgatacga tggccgagct tttcaggaaa ctgtcgttcg caggaatccc
     1981 gccctattac gttttccagt gcaggccgac caggggcaac cacacctacg ccgtgcccgc
     2041 ggaggaggga tacacagtct tcaagaaggc catagacagg gtatccggtc tggccaaacg
     2101 cgccaggttc gttatgtctc acgcgactgg aaagatagag tacgtgggca tcgacgaaaa
     2161 caagatatat atgaaatacc acagggcggc cgacccgaga agatacgatc tcttcatggc
     2221 cttcgacaga aatcccgacg cttactggct ggacgattac gtcgatcccg actcactggt
     2281 atagaaaagg cggcgaaggc cgtcttttca taaccacttc gtacaggcga tagcgagttt
     2341 cttcccgctt gaagtgatcc tgtgcagacc gattttcagg ccgtccagat cttcgaaacc
     2401 gctttcgact agcacttcct ctcccttcat ggattcatgc ttgtactgaa tgcagagctt
     2461 cttcaactcc ttgctctcga aatccccggg aacggtctgc attatccagc ggacgtacga
     2521 gacgttgttg gcatgaccgt tggtgtccag atccgactct ctcactttca atacggttga
     2581 aagatcggac tttccgggtt ctggcgggtc ttcgaatttg agcggttcgt tgaagtcttt
     2641 cgacagtcca tagacttcgt agtgtctgtc ggctatcctc accggtctgc gcctggaggc
     2701 gtcgatgagg atccactggg atttgccgtg cactatcagg gctcccgagt cgtcgataac
     2761 ctcgaaacgc ctgtatgcgt agaatttgtt catagaatag ggaacggttc tcacatttag
     2821 gttctccatg aaggccggag atctctgaat gtctatctcc cagcgaagca acagccacgc
     2881 gtacccgctg aaacgcatcg tttcgacgtc gtggccgacc tcaacgctct ggagggttac
     2941 aacgtcgttg aaatagtcca ttatggaggc gactgaagcc ttccagttcc tggtcagttc
     3001 gtagtacttc actctgtatc tctcttcggt gacgcgggga ttcaaaagaa aacctccaat
     3061 cctttgcaga acttaaacat gtgaacgtgt tatgctttta atagcaattc aaatctatca
     3121 cggtttcgac gggatgtctt gagaggttgt gaagatgctg gcttactgcg gaatagactg
     3181 ttcaaattgc ccggtttaca gggcgactat gacgaacgac gagatcctga agagagagac
     3241 ggccgataaa tggggaaggg agttcggttt tgttcttgaa aagagcgaca tgaactgcac
     3301 cggatgcagg agcgagatac tcttcaaact gtgcttcgac tgtcctttca aggactgttg
     3361 ttcgaagaag ggaatagaca actgcggcca gtgcgccgaa tacccctgtc agacactgga
     3421 aaggtttctt cgcccgatcc ccgaagcgag ggcgagcctg gacgaaatcc atagaaattt
     3481 tcacggaggt acttaattat gaaaaaaact ttcacactat taattcttca agtgatattt
     3541 gcagctatga tcctggcgct gacgctcgtt ccggtgaagg tcgaaaccgc ctcgggcccg
     3601 aaggacctca cttacgccac cgagttcttc gatctctgga acagtgttca ggattacacg
     3661 cacgcgaaag tgatcaacgt tgtcgatggc gatacccttc tggtgagcat agatggcgtt
     3721 gagacaaccc tgaggctcat cgggatcgat acgcccgaga ctgttcatcc caccaaagaa
     3781 gtcgagttct tcggaataga ggcctcgaac ctcgccaaag gcgttctctc cgacaaagag
     3841 ataatcataa gctacgactg gaaccccacg gacgtctacg gaagaaccct ggcgtacgta
     3901 tggctggaag tcgaatacta cggtttcaaa caatacatca tgtacaatct gctcggggtt
     3961 atcaacggtt atgggcgggc ctatctggcc ttcgccttcg acgagaaact tatggatctc
     4021 ttcgcccagg cccaggacat tccgagaact ctctctctcg gtctctggaa cgaacccgag
     4081 gatgaagagg aagagatcta ctacgacgga ccggttcaga tagcctatat attggccgat
     4141 tctctcgacg aatacgtgat gatatacaac ataggcgaca aacccgtcaa tctgaagggc
     4201 tggcagatac tggccgtcac caacggccag aaatacacct tcgacgacct gattctcgag
     4261 ccgggaatgg cggtccacat ccactccggt ccggaggcga gtgtaaacgt atggaaaaaa
     4321 agctacatct ggcgtaataa aaaggccgaa ggtagattgt tcgattcctc cggaaacctt
     4381 gtctcggtgt actcttattg atgttctcta tctatgggac gcatgaattt ttctgacaga
     4441 atttaaggat taagcaatct ataagaaagt tacttgatac ttcaacacaa cgcattatta
     4501 gcacaaacaa attagaatta ttttagttta taacccccct atccccctta gataggaacc
     4561 ctatatagtc cggatcatcc gatccggctt tttttgtttg ttttggtcta taattatctg
     4621 taatcctaac caaatatcgg aggtgcgccc ttgaaatgga tagacttgag aagtgatacc
     4681 gtgacgcttc cgggagaaga gatgcgcgcg gccatggcga acgccgaagt cggcgacgac
     4741 gtttacggcg acgaccccac cgtcaaccgt ctggaagaga tcgccgccga agtcctcgga
     4801 aaggaatcgg ccgttttcgt tccctcggga actttcggaa atcaactctc gatactgact
     4861 cataccctca ggggagacga ggtcataata ccggcctcga accacataat cgttcatgaa
     4921 gccggcgctt cggccgtcat agccggcgtt caaatgagaa cgctcgactg cgatgacgga
     4981 atgccctctg tcgatagaat actcaaagcg ataaggggag aggaccttca ctatcccaga
     5041 acgggattga tctgtctgga gaacgcgcat tctagcggaa gggttctccc tatggaatat
     5101 atgaaagagg tttattcgct tgcccgtgag cgcgcggttc cggttcatct cgacggagcc
     5161 aggatattca acgcggccgt ctctcttgga atagatgccg gagagatcgc ctcgaaagcc
     5221 gacagcgtca tgttctgcct ctccaaaggg ctcggggcgc cgattggctc catgctggtc
     5281 ggaacgaaag agttcatctc cagagcccgg aaaggacgca aaatcatggg tggagcgatg
     5341 cgacaggccg gcatcatagc cgcggccggg atcatggcta tcgagaagat gatcgatcgg
     5401 ctcggcgaag accacgaaaa tgcccggtat ctcgcgaaaa aactcgaaga aataaagggc
     5461 atcgaaatca tgagagacag actcgacata aacatggtct tcttcaagct cgaccagggc
     5521 agaccggagg ccatcgtcgg cgagctctac agggagggaa taaagataaa cccgcccgag
     5581 ggcgacgaaa ggaggctggt gaccaacctc gacgtttcac gctccgacct cgacacgttc
     5641 atcgctcgtt tcacgaaggc cgttaaatcc tgctaaaagc aacccgtatt aactttccag
     5701 ggggctcatc actacttcgg gtagtgatga gtcccttttt ttctcgaaat acatctatgt
     5761 atttatttgc cattatcgct aacaagcaac caaggacgac tctttcgctc tcccgctccc
     5821 tcccgacaaa tctcttcatg tctgttctcc ttcactatta tttttagatt ctaaaatgct
     5881 tccagacctg cttccgcaat ataagaaaag ttgcattagt tctttgtgtt agcagagaat
     5941 tttcttttgc acttctaaat agatagaacc ataaaacttg gttgactaga ttaaaaaaga
     6001 atctaagatg aggttttgtt ttttatgtta cgaagcgtat aattacagca aatgaatcct
     6061 gaatcatctt ttcgtagagg aacacaacat gcaattgatt tgaagcatcg cattatttta
     6121 ttacaaatga ttgtcttata aatactgttt ataaaacaag aattgaggtg atagtattgc
     6181 gattacttgg aacatatgag actccgggtc cctttcctct ggactaccgg aagggattca
     6241 tgtcgcttat caaggagtcc ctaaaagcct tctcagctga ggaatttgaa aggatttacg
     6301 gcaaacgtgc cggaagaaat atcgtcttca attgctactt tggggaggat atgcaaatag
     6361 ataaagaaaa cgagaatatc atcctcaaat cgccctcttt tctccttgaa gtctccagcc
     6421 cggataccaa actaatagca atgatctata acggcctcag gaagaatcgt gaatacacgg
     6481 tctttggttc caggataagg ctcaaagaaa taaagcctgt aaacactctt ataaggacga
     6541 aggataagat ccgcgtccgt tctgtctgtc ctatcctggt gaaatcgagc ccggatagtg
     6601 gatcaagata tctgctgcct ggagaagaga gcttctatgg cagtctaaac catttctgca
     6661 aggtgagata cgaagaattc ttctcagaag aattcaaagg gagcgtagag ctgcttcctc
     6721 ttgaactgaa aagagttgtc gtaaaacata tgggaaccta ccacactgga ttcaaaggca
     6781 ttttcgagtt atcctcgtct tctaaaatgc ttcagtt
//
LOCUS       DAOOLY010000120         6812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13258_length_6812_cov_4.115584, whole genome shotgun sequence.
ACCESSION   DAOOLY010000120 DAOOLY010000000
VERSION     DAOOLY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13258_length_6812_cov_4.115584"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="PLD15_07100"
     CDS             <1..1975
                     /locus_tag="PLD15_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63708.1"
                     /translation="SSTVLFSIYRKVRIIKLRFVSILSMFVLLIPLVACSDRTGYSVS
                     IKQARCEFAECYLDLDIRYPAKDRPEILITVDRIPVDAMDGVRTDDPKYSFKLESSGS
                     HKIYVTLVKGKRFLTSPVSTDILVDTRIPETPSIIWRVSGGMLLIHLATQGNISYTAF
                     KILVNSGIEKISTSPYFEIDIEKGRDYIIQGYTSRGVNQSEPGILDFLLNEDEAPKIN
                     TFIESPYSGGPIMLTLEDDWDRSSRLSVNAFAELSGIRFLYDGQRLIPESPLPQGEDA
                     IVVKVMDSSGNCTEYWESFNIVKRTSESLPELYIEEGAVRNAKWAAQDGTTVLQKFGG
                     GEWTTISTHDRDEKTTTIPRDSLSIEGDLYRLLVKSASEITLPSMPVFAKESFFKRFS
                     TEVISSLLGMDALLVGENDYRLFGNIVVRENSIMKVESGSTLTIARGNTLLVSGVLDL
                     EGRKDRIALNPGGSYGTLEVSNNGILIARNVDFNKTRIVVKNASVIAMDDCTLGAGLE
                     IRGARTVQLYNCEIAGDILFDGVIDLYIHSSRTGSGKFVLSNSLSSTVSRSKIENDTV
                     EIRNSRVSFLLGAVSSQNLKMHQLSRVSMTGLEISSEKLEVLEASSLSLEEIYSDGKF
                     LLSARTLSRVSLNEELAGELELETDEKSVIATY"
     gene            1962..2657
                     /locus_tag="PLD15_07105"
     CDS             1962..2657
                     /locus_tag="PLD15_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB63709.1"
                     /translation="MRRTEMKVFGDFDPGVLNGLIFDISYPLYRINHGTVLLSWFCKP
                     PGKTRRSLELGSGSGAISIYLASRYGITASGIEVDGQLHKVSLENARRNGVEDRVSFF
                     NCSVSDFLTSKRETHHDFDIVVSNPPHYLHPGIESPDSRRNTARRISPGLMNEFVRAT
                     GALLKNRGAFFFLVHPRDLTKWIRRMEDERLGIHRIRFAHGKSGGQAQLVLIAGRKNS
                     TSEILVEPPIILR"
     gene            2654..3295
                     /gene="tmk"
                     /locus_tag="PLD15_07110"
     CDS             2654..3295
                     /gene="tmk"
                     /locus_tag="PLD15_07110"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPB63710.1"
                     /translation="MNAGMFICFEGIDGSGKTTQIKLLQEYLSQLRVEHIIVREPGGT
                     KAGEEIRNILLHSDFRLKPESELLLFMAARAQIVREVIRPALEKGLVVLADRFMDSSL
                     AYQGIGRSIGIEIVRTINGFAVGSTIPDIIIYIDVPATEAVLRMRKEKKNDKIEVESL
                     EFFEKVRNGYMKIIREKLDNYEIVDGTRSMIEVHECIRNIIDRFLQRFRLNGR"
     gene            3302..5797
                     /locus_tag="PLD15_07115"
     CDS             3302..5797
                     /locus_tag="PLD15_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPB63711.1"
                     /translation="MYFDKFSEGAAQVFVTAQDEARGLGHPYVGTEHLLLAIIKVDNE
                     QTTKILRNYSITYERVSREVTSMVGTNVHQSVVGAPQMTPRARRVIELANDESKMLGQ
                     DKIDVEHILLGIVREGEGIAAHILKQMGVNLSQLRREIIENITGDWDDGGSEQPENVF
                     SPQQNTSTSAAIKQLEGFGTDLTSMARTGKLDPIIGREIERQRLMEVLSRRKKNNPVL
                     IGEPGVGKTAIVEGLAQMIVDEEIPETLKNKVIFALDVASLIAGTKYRGEFEKRLKKL
                     IHVVKSNGNIILFIDEVHTIVGAGSAEGAVDAANILKPALASGEIRCIGSTTPDEYRK
                     YIEKDAALERRFQKIYVTEPSPEQSVEILKGIRHKYELHHKVKYTEIALEAAVMLSHR
                     YVSDRFLPDKAIDIIDEAGSRARLQKLTIPEELKKMQTELELLKIKRETAAADQEYEK
                     AAQLKEKERILQQKYRDSYNQWRSRVDSEIVTLDEDEIAEIVSNWTGIPLRKLEETDT
                     EKLLTLESALHERIVSQDEAISAVAKSIRRARSGLKDPRRPIGTFLFLGPTGVGKTEL
                     AKALAEYLFGDDKSLVRFDMSEYMERFSVSRLIGAPPGYVGYDEGGTLTERIRKRPFS
                     VILFDEIEKAHPDIFNILLQIMDDGRLTDSQGRQVDFRNTIVIMTSNLGGEFINKTKT
                     SIGFVESTRQDREYEAMKTSVLEEVKKTFRPEFLNRLDEIVVFHQLTKEQIKVIIDIL
                     MRDMRKRLKDKHLELVLTDKAQDFLVEEGFNPVYGARPLKRAIQKYVEDPLSEELLRG
                     RFHEGDMITIELKNKLLVFTRKKDEKAEVSATT"
     gene            5909..>6812
                     /locus_tag="PLD15_07120"
     CDS             5909..>6812
                     /locus_tag="PLD15_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPB63712.1"
                     /translation="MKKKQSYVCDACGYESPAWFGKCPSCEEWNTAVAFNPTDNSSSQ
                     ETRQPDIKPLSDVSTVESPRLRSGIEEFDRSIGGGIVPGSAVLISGEPGIGKSTFMLQ
                     LSNYIARDSQILYVSGEESPRQLKMRADRLGLANTGNIHIMSANDISFIRAGDISRYA
                     LMVFDSLQTLKLSDVPSLPGSIVQVRECANALVSNAKSSEVPVFIVSHVTKGGQIAGP
                     KLIEHLVDTVLYFEASKTGYRFLRTVKNRFGPSDEIAVFEMTSSGLKEIADISSVFVE
                     DFISSPGNTIAVISEGSRAFLVEIQ"
BASE COUNT         1976 a         1535 c         1774 g         1527 t
ORIGIN      
        1 atcctcaact gtgttattct ctatttatag gaaggtgagg attataaagc tcaggtttgt
       61 ctcgattcta tcaatgttcg ttctcttgat tccactggtt gcctgcagcg accggaccgg
      121 ttattctgtt tcaatcaagc aggctaggtg tgaattcgcc gagtgttatc tcgatcttga
      181 tataaggtat cccgcgaaag ataggccgga gattcttatc accgttgaca gaatacccgt
      241 agatgcgatg gacggagtca gaactgacga tccgaagtac agtttcaagc ttgaatcgtc
      301 gggttcccac aagatatacg taactctcgt aaagggcaag aggttcttga ccagtccggt
      361 ttcaaccgat atcctggtcg ataccagaat ccccgaaaca ccatcaatca tctggagggt
      421 gtctggagga atgctgttga ttcacctggc cacgcaaggg aacatctctt atacggcctt
      481 caaaatactt gtcaactccg ggattgaaaa gatctccaca tctccatatt tcgagatcga
      541 tatagaaaag ggaagagatt acatcataca gggttacact tcgcgcggcg taaaccagtc
      601 agagccaggg attctagatt tcctgctcaa cgaagatgaa gcgcctaaga taaatacctt
      661 catagagtct ccatactccg ggggcccgat tatgctcaca ctggaagacg actgggatag
      721 aagcagtagg ctttcggtga acgctttcgc agaactgagc ggtattagat tcctctacga
      781 tggccagaga ttgattcccg aatcgcctct accccagggt gaggacgcga tagtggtaaa
      841 ggtgatggac tcttcaggca attgtacaga gtattgggag agtttcaaca tagtcaagag
      901 aacctcggag agccttccgg agctgtatat tgaagaaggg gcagtacgaa acgctaagtg
      961 ggctgcgcag gacggaacaa ccgttttgca gaagttcggc ggtggtgagt ggaccaccat
     1021 ctcgacccat gaccgggacg aaaaaaccac caccatccct agagactctc tttctatcga
     1081 aggcgatctt tacaggttgc tcgtaaaaag cgcttcagag atcacacttc catcgatgcc
     1141 ggtctttgca aaagaaagct tcttcaaaag attcagcaca gaggtaatct catcgctttt
     1201 gggaatggat gcccttctgg taggagagaa cgactatcgc ctctttggaa atatcgtggt
     1261 gagagagaac tccataatga aggtcgaatc gggttctact ctgaccattg caagaggtaa
     1321 caccctcctc gtgagcggtg tccttgatct ggaaggtaga aaggatagaa tagccctgaa
     1381 ccccgggggt tcttacggaa ctttagaggt ctcaaacaac ggtattttga tcgccaggaa
     1441 cgtcgatttc aacaaaacgc gaatagtcgt aaaaaacgcc tcggtgatcg caatggatga
     1501 ttgcactctg ggagccggtt tggagatacg cggcgccaga accgttcagt tgtacaactg
     1561 tgagatagcc ggagacatac tcttcgacgg tgtgatagat ctttacatac attcatccag
     1621 aaccggctcc ggaaagttcg ttttgagcaa ttctttatct tctacggttt ccagatcgaa
     1681 gatagagaac gatacggtag aaatcaggaa ttcgagagtg agtttccttc tcggagctgt
     1741 ttcgtcgcag aatttgaaga tgcatcagtt gtccagagta tctatgaccg gactcgagat
     1801 atcctctgag aaacttgaag tgcttgaagc ctcctcgcta agcctggaag agatttacag
     1861 cgatggaaag ttcttactgt cagcaagaac tctctcgaga gtaagtctta atgaagaact
     1921 cgccggagaa ctggaacttg aaaccgacga aaagtcggta attgcgacgt actgagatga
     1981 aggtttttgg agatttcgat cccggagtcc tcaatggact gatttttgac atttcctacc
     2041 cgctgtacag aataaaccac ggaacggttt tgctatcatg gttctgcaaa ccgcccggaa
     2101 aaacccgcag atcactcgag ctgggttctg gaagcggggc gatttccatc tacctggctt
     2161 caagatacgg cataaccgct tcgggaatcg aagtggatgg gcaattacac aaagtatctt
     2221 tggagaatgc ccgaagaaac ggtgtggaag acagggtctc ttttttcaac tgttcggtca
     2281 gtgattttct cacttccaaa cgtgagacgc accacgattt cgatatagtg gtatcgaacc
     2341 ctccacacta ccttcacccg ggaatcgaga gtcccgacag tagaagaaac acggccagga
     2401 gaatatctcc cgggctcatg aacgagttcg tcagagcgac cggggctctc ttgaagaaca
     2461 ggggagcttt cttttttctc gtccatccca gagacctcac gaagtggata aggagaatgg
     2521 aggacgagag actcggaatc cacagaatca gatttgcgca cggcaagagt ggaggacagg
     2581 ctcagctagt tttgattgct ggcagaaaga actccacgtc cgaaatactg gtagaaccgc
     2641 caataatttt gaggtgaatg caggaatgtt catttgtttc gaaggaatcg acgggtctgg
     2701 aaaaacgact caaatcaaac ttttacagga atacttgagt caattaagag tagaacatat
     2761 aattgtaagg gaaccgggag gcaccaaagc cggtgaggag ataaggaaca tcctactcca
     2821 cagcgatttc aggctgaagc cggagtctga gttgctgttg tttatggcag caagagccca
     2881 gatagtgaga gaagtcatca gacctgcgct tgaaaaagga cttgtggtgc tggcagacag
     2941 gttcatggat tcctcgctgg cgtaccaggg aataggtaga tcgattggaa tagaaatagt
     3001 cagaaccata aacgggtttg cagtcggttc aactatcccg gatatcatta tatacatcga
     3061 tgttcctgca actgaagctg tcttgcggat gagaaaagaa aagaaaaatg ataaaataga
     3121 agtggagagt ttggagtttt tcgaaaaagt aaggaacggt tatatgaaaa ttatcaggga
     3181 gaaattggat aattatgaaa ttgtggatgg aaccagaagt atgatagagg ttcacgaatg
     3241 tatcagaaac attattgatc gttttctaca gagattcagg ctcaacggga ggtgaaatag
     3301 aatgtatttc gacaagtttt ctgaaggcgc cgcgcaggtt ttcgtaacgg cacaggacga
     3361 ggccagaggt ctcggccacc cgtatgttgg aaccgaacac ctccttctgg caatcatcaa
     3421 ggtcgataat gagcaaacaa ccaagattct caggaactat tccataacat atgaaagagt
     3481 ctccagagaa gtgacttcga tggtaggcac caatgttcat cagtctgtag tcggagcccc
     3541 acagatgacc cccagggcga gacgagtcat agagcttgca aacgatgaat caaaaatgct
     3601 cggtcaggac aagatcgacg ttgaacatat tttgcttggt atcgtacgcg aaggagaagg
     3661 gatcgcagcg catattttga agcagatggg tgtgaacctc tctcaactca ggagagagat
     3721 tatcgaaaac atcaccggag actgggacga cggcggctca gaacaaccgg agaacgtttt
     3781 ttctccgcag caaaacacga gcaccagcgc ggctatcaaa cagctcgaag gttttggaac
     3841 agatctgact tcgatggccc ggaccggaaa gctcgatccg ataataggca gagagataga
     3901 acgccagagg ttaatggagg tcctatccag aagaaagaag aacaatcccg tgttgattgg
     3961 cgaaccggga gttggaaaaa ccgcgattgt cgaaggtctg gcacagatga tagtcgatga
     4021 ggagataccc gagactctca agaacaaggt tatcttcgcg ctcgacgtgg cctctttgat
     4081 agccggaacg aaatacaggg gagagttcga gaaacgtctg aagaaattga ttcatgtagt
     4141 caagtccaac ggcaacatca tacttttcat cgacgaagtg cacacgatcg tcggagccgg
     4201 ttcggccgaa ggtgccgtgg acgccgccaa tattttgaaa cccgcactgg caagcggaga
     4261 gataaggtgc attggctcta cgactcccga cgaatacagg aagtatatag agaaagacgc
     4321 cgccctggag agacgtttcc agaagatata cgttaccgaa ccgtcaccgg agcagtccgt
     4381 cgagattctg aagggaataa gacacaagta cgaattacac cacaaagtca aatacacaga
     4441 gatagcactg gaagcggccg tgatgctttc ccatcgctac gtttccgata gattccttcc
     4501 cgacaaagcg atagatataa tagatgaagc cggttcgagg gcaagacttc agaagttgac
     4561 catacctgaa gagctcaaaa agatgcagac cgagctggag ctgttgaaaa taaagaggga
     4621 gacggccgca gctgatcagg agtacgaaaa ggctgcccag ttgaaggaga aggaaagaat
     4681 actccagcag aagtacagag atagctacaa ccagtggcgc agcagggtcg atagcgaaat
     4741 cgtgacactc gatgaagacg agatagcgga aatcgtttcc aactggaccg gtattcctct
     4801 caggaagctc gaagagacgg atactgagaa gctactaact ctagagtcgg cccttcatga
     4861 gagaatagtc tctcaggacg aggctatctc cgccgtcgcc aaatccataa gacgtgcccg
     4921 cagcggcctc aaagacccca gaagaccgat aggcactttc ctcttccttg gacctacagg
     4981 tgtggggaag accgagctgg ccaaggcgct ggccgagtat ctcttcgggg acgacaaatc
     5041 tctggtcaga ttcgatatgt ccgagtacat ggaacgcttc tcggtatcga gacttatcgg
     5101 ggctcctccg ggatatgtcg gttacgacga aggtggaact ctcaccgaaa gaatcaggaa
     5161 gagacctttc tctgtgatac tgttcgacga aatcgagaag gctcacccgg acatattcaa
     5221 tatccttctc cagattatgg acgacggacg gctcaccgat tcacagggaa ggcaggtcga
     5281 tttcaggaac accatagtaa taatgaccag caatctggga ggtgaattca tcaacaagac
     5341 caagacttcg attggtttcg tggaatccac ccgtcaggac agggagtacg aggctatgaa
     5401 gacctccgtc ctggaagagg tcaagaagac tttcagaccg gagttcctca acaggcttga
     5461 tgaaatcgtt gtattccatc aactgactaa ggaacagata aaggtgatta tagatatcct
     5521 tatgagagat atgcgtaaac gtctgaaaga taagcacctg gaactggtac tgacagacaa
     5581 ggcccaggat ttcttggttg aggaaggatt caacccggta tacggtgcga gacctctaaa
     5641 gagggccata cagaagtatg ttgaagatcc gctctcagag gagctccttc gcggaaggtt
     5701 ccacgagggc gacatgatca ccatcgagtt gaagaacaag ctactggtct tcaccagaaa
     5761 aaaagatgaa aaggcggaag tcagtgctac aacttgatct acagttacga ccgttatatt
     5821 gactaaaaaa gggggcacat ttattgtgcc ccctgtgtat aataaatcga aagatacatg
     5881 aagaaaggag agctcgatcc agaagtctat gaagaaaaaa caaagctatg tctgtgatgc
     5941 ctgcggttac gaatcaccgg cctggttcgg gaagtgtccg tcctgcgaag agtggaacac
     6001 ggcggtagcc ttcaatccca ctgataacag ctcctctcag gagactcgcc aaccggacat
     6061 aaaacccctt tcagacgtaa gtactgtaga atcgccgcgc ctcaggagcg ggatagagga
     6121 gttcgacagg tccatcggag gcgggatcgt tcctggaagc gcagttttga taagcggaga
     6181 accgggaatc ggaaaatcca ccttcatgct tcagctctcc aactacatag ccagagacag
     6241 tcagattctc tacgtttcag gcgaagaatc cccccgacag ttgaaaatga gagccgacag
     6301 actcggcctg gcgaacaccg gaaacatcca tatcatgtcg gcaaacgata tctccttcat
     6361 cagagcggga gacatctcca ggtacgctct catggtcttc gactctcttc aaacactcaa
     6421 gctatcggat gtcccttccc tacccggctc gatagtgcag gttagagagt gcgcaaatgc
     6481 cctggtgtcg aacgccaaga gctccgaagt gccggttttt atcgtctcgc atgtaactaa
     6541 gggcggacag atcgccggac ccaagttgat agagcatctg gttgacacgg ttctatattt
     6601 tgaagccagc aagacgggat atcgcttcct gaggaccgtg aaaaaccgtt tcggtccatc
     6661 cgacgagata gccgtattcg aaatgacctc ctccggcctc aaggagatcg ccgatataag
     6721 cagcgttttt gtcgaagact tcatcagttc gccggggaat acaatagcag tgatctccga
     6781 gggatcgagg gcttttctag tggagatcca gg
//
LOCUS       DAOOLY010000121         6806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13273_length_6806_cov_4.897793, whole genome shotgun sequence.
ACCESSION   DAOOLY010000121 DAOOLY010000000
VERSION     DAOOLY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13273_length_6806_cov_4.897793"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..2006)
                     /gene="groL"
                     /locus_tag="PLD15_07125"
     CDS             complement(384..2006)
                     /gene="groL"
                     /locus_tag="PLD15_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731609.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPB63713.1"
                     /translation="MAKIIKYSEDARRALERGVDTVAEAVKITLGPKGRNVVLEKSWG
                     SPTITNDGVSIAKEIELEDKFENLGAQLVKEVASKTNDIAGDGTTTATVLAQAMIKEG
                     LKNVTAGANPILLKKGIQKATERAVAHIRSLSKKISSREDIASVAAISANDTEIGELI
                     AEAMDKVGQDGVITVEDSKTLETYVEFVEGMQFDRGYISPYFVSDPEKMEAIIKEPLI
                     LITDKKLSAVKPIVPMLEKVAQSGKPLVIIAEDIEGEALSTLVLNKLRGTLDSVAVKA
                     PGFGDRRKAMLQDIATLTGGTVISEEVGLTLENATINDLGSAGMVKVKKDDTIIVDGK
                     GDADKIKQRIGQIKAQIEQTTSEYEKETLQERLAKLSGGVAVIKVGAATETELKEKKH
                     RIEDALSATRAAVEEGIVAGGGVVLARAKKVLQELLDSTDNPDIKVGIKVVLKALDTP
                     MKQIVENAGLDGAVVVDKILNNDDPAFGYDALNDVYTDMFKVGIIDPAKVTRSALQNA
                     ASIAGILLTTEAALTEKPEEKPQAPMPQMPEY"
     gene            complement(2020..2289)
                     /gene="groES"
                     /locus_tag="PLD15_07130"
     CDS             complement(2020..2289)
                     /gene="groES"
                     /locus_tag="PLD15_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPB63714.1"
                     /translation="MKVVPLGSRLLIKPYEEEKRTSGGIVLPDAAKEKQMTAKVIAVG
                     EKVEDIDVKENDKVLYSKYSGTEIKIDDEDYIIIDQDDILAKIEG"
     gene            complement(2518..3936)
                     /gene="pyk"
                     /locus_tag="PLD15_07135"
     CDS             complement(2518..3936)
                     /gene="pyk"
                     /locus_tag="PLD15_07135"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPB63715.1"
                     /translation="MRKTKIVCTIGPATESPEMLTKLVFRGMNVARLNTTHGDLEEHR
                     ERIRRIKEVRNSLKVPLSLLLDLSGPKIRTGSFKRERVELLSGRDFLLTTSAIEGDEN
                     AVSINYDRLPGEVKPGHMILMDDGKIKLKVFSTDENHILTKIVNGGIVTHRRGINLPG
                     IDIGIPAVTDKDRRFIKLGIEEGVDYFALSFVRKAEDVILAREIVTDSHGSIPIISKI
                     ETAQALKSIEEIAAVSDGLMVARGDLGVEIPVEEVPVAQKKIIKFGNYNRIPVITATQ
                     MLESMIENPVPTRAETTDITNAIVDGSDAVMLSGETSIGKHPLEAVAVMDLTARATER
                     YLNQNPDILWWTREKVLTDDHTDAICRAAWDISEALKVKVIVSSTFSGHTARNVSGFR
                     PRAHILAITPNEETYFRLGLIWGAHPVLMGLGESTDDLVKVAGPLAKKLKLVKKGDTI
                     ILTAGIPFGTSRSTNILKLEKT"
     gene            complement(3946..4908)
                     /gene="pfkA"
                     /locus_tag="PLD15_07140"
     CDS             complement(3946..4908)
                     /gene="pfkA"
                     /locus_tag="PLD15_07140"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPB63716.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRAAVRTAVTDGLEVVGIHRGYSG
                     LLDEDFTDMDYSSVGGIMERGGTILRSSRCEEFRLEEGRAKAADILKKHGIGGLVVIG
                     GEGSLSGAKLLMEEQGIPVVGLPGTIDNDIALTDMCIGVDTCLNTCVDTIQKLKDTAS
                     SHERAFVVEVMGRSSGYVALASGMAVGAEAIIIPEIPVNYEEIADKIWRERKRGKINC
                     IIVVAEGASSAYTVARHVEHRIGYETRITILGHIQRGGSPTAFDRVLASRMGYASIKA
                     LEESEAGTMIALQGGRMVRVSLEKILSQKKTIDMELVEMAKILS"
     gene            5056..5286
                     /locus_tag="PLD15_07145"
     CDS             5056..5286
                     /locus_tag="PLD15_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPB63717.1"
                     /translation="MKVLKSGVPVYEAKMYQEILLKEGIFAELVDSHFAYTDSIYFGS
                     GGMVDIIIPDDDYETAVSIFEDLKVEGREDPE"
     gene            5283..6389
                     /gene="gcvT"
                     /locus_tag="PLD15_07150"
     CDS             5283..6389
                     /gene="gcvT"
                     /locus_tag="PLD15_07150"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPB63718.1"
                     /translation="MSDLKRTPLYKEHLRLNGKIVDFAGWEMPLQFDSIIEEHNLVRT
                     KAGLFDVSHMGEIEIVGSEAISYSDYLVSNSVDTMKNGEIVYSPMCNEKGGVVDDVLV
                     YRISNNKTLFVVNASNKDKDYDWIHKNSTGFDVQVKDVSDNYAQIAFQGPLAEEILSE
                     ISQVRLDQIPFYHFAEGRVNGIKALVSRTGYTGEDGFELYTEPDAAIPLWRKLLEIGG
                     PKGVKPIGLAARDTLRFEACYMLYGNELNDHNTPLEAGLKWTVKLDKNFIGRDVLARQ
                     SEEGTEYRLKGIEIAGKAIARHGYEIVDGDNVIGWVSSGLFSPTLGKSLAMAYLKKDY
                     WKLGTQVGVSVRGKVVPANVVKTPFYRGSVKSKG"
     gene            6406..6777
                     /gene="gcvH"
                     /locus_tag="PLD15_07155"
     CDS             6406..6777
                     /gene="gcvH"
                     /locus_tag="PLD15_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPB63719.1"
                     /translation="MKKYTKTHEWVSVEGDIATVGISDHAQDHLGDVVYIDLPEAGKS
                     LKKGDVLCTIESVKAASDVYAPVSGNIVEVNAGLDSSPETINSDAEGAGWIAKIKLSS
                     IAELDSLMDLEAYKKHCEEEG"
BASE COUNT         1554 a         1723 c         1638 g         1891 t
ORIGIN      
        1 ccttaagctt ctcggtgagt aaactcaaag ttagtctcct aattcttcga atacggtact
       61 attctaattg ggcggacatt atccttccaa agacccgtcc ggctctgacg tgttgtaggt
      121 ttattcggga ttcccgtcat tcgaaaacta aaggttcttt gttgaagttg ttggctcaat
      181 tatacctttt cgatcctttt ttgtggacag aaatttccga ttaacgcatg aagagaattg
      241 gcactttgac ttagcaaaag agctgtcctt ggaagagccg tccttggaaa gaacagaaga
      301 gtggttcttc gaaacgtata agttggaaaa aaagggagag caacgccctc ccttatttta
      361 tgagtatcgc ttttctattt ctatcagtac tccggcatct gtggcatcgg cgcctgaggc
      421 ttttcttcag gcttttctgt cagcgcggct tccgtggtca gaaggatgcc ggcgatcgaa
      481 gctgcgttct gaagcgcgct tctggtaacc tttgccggat cgattatacc gaccttgaac
      541 atatcggtat aaacgtcgtt gagagcgtcg taaccgaaag cggggtcatc gttgttgagg
      601 atcttgtcca caacgaccgc gccgtcgagt ccggcgtttt ccacgatctg tttcatgggt
      661 gtatcgagag ccttgaggac taccttgata ccgactttga tatccgggtt atccgtgctg
      721 tcgagaagct cctgaagtac cttcttggcc cttgccagaa cgacaccgcc tccggcgacg
      781 attccttctt caacggctgc cctggttgcg gagagggcat cttcgattct gtgcttcttc
      841 tccttcaatt cggtctcggt ggccgctccg acctttataa ctgcaactcc accggagagt
      901 ttggcgagcc tctcctgaag ggtttccttt tcgtattcgg aagttgtctg ctcgatctga
      961 gcctttatct gtccgattct ctgctttatc ttgtcggcat cgcccttgcc gtcaacgatg
     1021 atcgtgtcgt ctttcttaac cttcaccatg ccggcgcttc cgaggtcgtt gatggtggcg
     1081 ttttcgagag ttaaaccgac ttcctcgctg atcacagtac cgccggtgag cgttgctata
     1141 tcctgcaaca tggcctttct cctgtctccg aaaccgggag ccttcacggc aacggagtca
     1201 agagttcctc tcaacttgtt gagaacgaga gtggagagcg cttcgccttc gatatcttcg
     1261 gcaattatta ccagaggctt tccggactgt gcaacctttt ccagcatcgg aacgatcggc
     1321 ttaaccgccg aaagtttctt gtcggtgatg agaatgagcg gttccttgat gatagcttcc
     1381 atcttctcgg ggtcggaaac gaagtaggga gagatgtagc ccctgtcgaa ctgcatgcct
     1441 tcgacgaatt cgacgtaagt ctccagcgtt ttggaatctt cgacggttat gactccatcc
     1501 tgtccgacct tgtccatggc ttcggctatc agttcgccga tctctgtatc gttggccgag
     1561 atagccgcga ccgacgctat gtcctctctg gaggagatct tcttggaaag ggatctgatg
     1621 tgtgccaccg ctctttcagt tgccttctgg ataccttttt tgagaaggat cgggttggcg
     1681 ccggcggtaa cgttcttgag accttccttt atcattgcct gtgccagaac cgtggcggtc
     1741 gtggtaccgt caccggctat atcgttggtc ttggaagcca cttctttgac tagctgagca
     1801 ccgaggtttt cgaacttatc ttcgagctcg atttctttcg cgatggatac tccgtcgttg
     1861 gttatcgtgg gagaacccca gctcttttcg agaaccacat ttctgccctt gggaccgaga
     1921 gttatcttta ccgcctcggc tacggtgtcc actcctcttt ccagagccct gcgagcatct
     1981 tcactgtatt ttattatctt agccataccg cttcctcctt cacccttcga tctttgccag
     2041 gatatcgtcc tgatcgatga ttatgtagtc ttcgtcatcg atcttgatct ctgttccgga
     2101 atacttggaa taaagcacct tgtcgttttc tttcacatcg atatcctcga ccttttctcc
     2161 gaccgctata acctttgcgg tcatctgttt ctcttttgcc gcatctggaa gaactatacc
     2221 gccagatgtt ctcttctctt cctcataagg cttgattaac aatctcgaac caaggggaac
     2281 tactttcatc taaaaagcct ccttccaatc tgtatagtta gcactcgata tatgagtttg
     2341 ctaattaatg ataaaacaac atcacgattt attcaagcac cttttttgtt aaatgatgtt
     2401 gaccagagaa gattatacat ctatagagac aattattcca gcaaatctcg ttatttctcg
     2461 ctttataaat aaaaaatggc aggaaatctc tagatcctgc cattcaaaga ttttctctca
     2521 agttttttcc agttttaaga tgttggtgga tctgctggtt ccaaagggaa taccggcggt
     2581 gaggattata gtatcgcctt tcttgacgag cttgagcttt tttgccagtg gcccggcgac
     2641 ctttactaga tcgtctgtcg attctcccag gcccatcagc accggatgag ctccccagat
     2701 caagcccagt ctgaagtaag tttcttcatt aggtgtaatg gcaagaatat gagcccttgg
     2761 cctgaaacca gatacgtttc tcgcggtatg gccggagaaa gttgagctga caataacttt
     2821 cactttcagc gcttcgctta tgtcccaggc ggccctgcag atagcatcgg tgtgatcgtc
     2881 ggtcaggact ttttcgcgcg tccaccatag tatgtcggga ttctggttaa gatatctctc
     2941 cgttgccctg gcggtaagat ccataaccgc gaccgcttcg agcggatgtt tccctatcga
     3001 tgtctccccc gaaagcatta cagcatcgct tccatctacg atagcgttgg ttatgtcggt
     3061 cgtttcggct ctggtgggca cggggttctc tatcatcgat tcgagcatct gtgttgccgt
     3121 gatcaccggt attctgttgt aatttccgaa ttttattatc tttttctgag cgaccggtac
     3181 ttcttcgacc ggtatttcga ccccgagatc gcccctggca accatcaggc cgtcggaaac
     3241 ggcggcgatt tcttcgatgc tctttagtgc ctgcgccgtt tctattttcg agatgatcgg
     3301 tatggaacca tggctatcgg tcacgatttc acgggccagg ataacgtcct ccgcctttct
     3361 tacgaaagag agggcgaagt aatcgactcc ttcttcgatg cccagcttta tgaaccttct
     3421 gtctttatct gtgaccgcag gaattccgat gtcgattccc ggaaggttta tacctcttct
     3481 gtgagtgacg atgcctccgt tgacgatctt cgttagaatg tggttttcgt ccgtagaaaa
     3541 gaccttcagc tttattttgc cgtcgtccat caatatcatg tgtccgggtt tgacttcacc
     3601 tggcagacga tcgtagttta tgctcaccgc gttttcatcg ccctcgatag ccgaggttgt
     3661 caggagaaag tccctaccgg aaaggagctc gactctctct cttttgaagg aaccggttct
     3721 tatcttgggt ccggaaagat ccagaagcag cgacagaggg actttcagag agttgcggac
     3781 ctccttgatt ctacgaatcc gctctctgtg ttcctccagg tctccgtgtg tcgtgttcag
     3841 tctggcaacg ttcattcccc tgaaaacgag ttttgtcagc atctccgggg attccgttgc
     3901 cggaccgatt gtacagacta tcttcgtttt tctcatttcg cacctttaag atagaatttt
     3961 ggccatctcg accagttcca tatcgatcgt tttcttttga gaaaggatct tttcaagcga
     4021 aactctgacc attcttcctc cctgaagagc gatcatagtg ccggcctcgc tctcctccag
     4081 agcctttatg gaggcgtaac ccattctaga tgctagaacc ctgtcgaagg ccgttggcga
     4141 tcccccgcgc tggatatgtc ccagaatggt aattctggtt tcgtacccta tcctgtgttc
     4201 tacatgcctg gcaaccgtgt aagcgctcga ggcaccttcg gccacgacta ttatgcagtt
     4261 gatctttccg cgcttcctct cccgccagat cttgtcggcg atctcctcgt agttcaccgg
     4321 aatctccgga attattattg cctcggcgcc tacggccatt cccgaggcca gggccacgta
     4381 acccgagctc ctgcccataa cctccacaac gaaggcgcgt tcatgcgaag aggccgtatc
     4441 tttcagtttt tgaatggtat ccacgcaggt attcaagcat gtatcaaccc ctatacacat
     4501 atccgtcagg gctatgtcgt tgtctatagt gccgggaagt ccgaccacgg gaattccctg
     4561 ctcttccatc aaaagtttgg ccccactgag actaccctct ccgccgatga ctaccagtcc
     4621 gcctatcccg tgttttttca gtatatcggc cgcctttgcc ctgccctctt cgagtctgaa
     4681 ctcctcgcat ctagaactgc gcaggatggt tccccctctt tccattatcc ctccgacaga
     4741 agagtaatcc atgtccgtga agtcttcgtc gagaaggccg gagtagcctc tatgaattcc
     4801 gacaacttcc agaccgtccg ttaccgcagt cctgaccgct gcccttatcg ccgcgttcat
     4861 ccccggagca tcaccgccgc tggtcagaac tccgattttc tttatcatca atatccctcc
     4921 tgagttttca atcttgctgt acgccgtaat tttagcatat cagcatatta atttcacgag
     4981 agacgaagct tcactaagtt aactaataac atgagttggt gtataatctc aagtgaaaca
     5041 gggggagtgg ttataatgaa ggttcttaag tcgggagttc ccgtgtacga ggcaaaaatg
     5101 taccaggaaa tacttctcaa agaagggatc ttcgcagagc tagtcgattc acatttcgcg
     5161 tacaccgaca gtatctattt tggatctggc ggcatggtgg acataattat tccagacgat
     5221 gactacgaaa cagctgttag tatatttgaa gacttaaaag tcgaagggag agaggatccg
     5281 gaatgagtga tttgaaaagg acacctttgt acaaggagca tctcaggctg aatggtaaga
     5341 tagttgactt cgcaggttgg gaaatgcctc ttcaattcga ttcgatcatc gaagagcaca
     5401 accttgtcag gactaaggcg ggactcttcg atgtctctca tatgggagag atagaaatcg
     5461 taggttccga agcaatttcc tactccgatt acctggtcag caactccgtt gacactatga
     5521 agaatggtga gatagtatat tcacccatgt gcaacgaaaa gggcggagtg gtagacgatg
     5581 ttctggttta cagaataagc aacaataaaa ctctctttgt ggtgaacgct tccaacaaag
     5641 acaaagacta cgactggata cacaagaact ccacaggttt cgacgtgcag gtgaaggatg
     5701 tttcggacaa ttatgcccag atagccttcc agggaccgct agccgaggag atactttccg
     5761 agatttccca ggtaaggctc gaccagatac ccttctacca tttcgccgaa gggcgggtca
     5821 acgggataaa ggctctggtt tctagaaccg gttacaccgg tgaagacggt ttcgaacttt
     5881 acaccgaacc ggatgcggcc atacccctgt ggagaaagct cctcgagatc ggtggaccta
     5941 aaggcgtaaa gccaatcggc ctcgccgcca gagatacgct cagattcgaa gcctgttaca
     6001 tgttgtacgg aaatgaattg aacgatcata acacgccgct ggaagcggga ttgaaatgga
     6061 ctgtgaaact cgacaagaac ttcataggta gagatgtact ggccaggcag agcgaagagg
     6121 gaaccgaata caggttgaaa ggcatcgaga ttgccggaaa ggccatagcg cgccacggtt
     6181 acgaaatcgt cgacggagat aacgtgatcg gctgggtttc tagcggtctc ttctcgccca
     6241 ctctgggaaa atctctcgcg atggcttacc tgaagaaaga ttactggaag ttaggcaccc
     6301 aggttggcgt ttcggtgagg ggaaaggtag taccggcaaa cgttgtgaag acacctttct
     6361 atagaggttc cgtgaagtca aaaggataaa aaggaggagt aaaaaatgaa gaagtacaca
     6421 aagacacacg agtgggtatc tgtcgaagga gacatcgcga cggtaggtat ttccgatcat
     6481 gcccaggatc atctcggaga tgtggtctat atagacctac ccgaagcagg caaatccctg
     6541 aagaaggggg acgttctctg cacgatcgaa tctgttaagg ctgcaagcga cgtgtacgca
     6601 cccgtgagcg gtaacattgt ggaggttaac gccggactcg attcctctcc cgagacgata
     6661 aacagtgatg ccgaaggcgc cggctggatc gcaaaaatca aactgagcag tattgccgag
     6721 ctcgacagcc tgatggatct ggaggcttac aagaagcatt gtgaagagga gggctaatat
     6781 gcctgaattc ccgtatcttc ctcaga
//
LOCUS       DAOOLY010000122         6795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13304_length_6795_cov_4.160534, whole genome shotgun sequence.
ACCESSION   DAOOLY010000122 DAOOLY010000000
VERSION     DAOOLY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13304_length_6795_cov_4.160534"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PLD15_07160"
     CDS             complement(<1..1174)
                     /locus_tag="PLD15_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB63720.1"
                     /translation="MAINTVVLVFGTAVSLFLLFFAFKALKKKRLVDDTPTSKAHGVF
                     IGLVEVSGKVECPSPLRSYLTESECVYYRWNVSEHWSKTVTETYTDSQGKTKTRTRTE
                     SGWKTIASDEQMSHFMLRDESGAIQVRPEDSKVDGECILNYYCGPSDPMYYGKGPAGS
                     IMNSDYRRCFTETIIPNGSTLYVMGQAREREDKVAAEIAYDREAPVFLISMRDEKKVS
                     RSYGFNYWLLALGGLAVAFFTVFISNRTGYGWIPFSYMNQIIAAGLYLVAWTIGWFWT
                     VFNALKSLRERTRQGKSQIEVQLKRRKDLVPSLVSTVLGLMRHEQGVQEALASLRSEA
                     YNGFEGVGGRLLALIEKYPSLKSSEVVLKLQKELADTETRIELSRAYYNDIVTFYNT"
     gene            complement(1190..2416)
                     /locus_tag="PLD15_07165"
     CDS             complement(1190..2416)
                     /locus_tag="PLD15_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HPB63721.1"
                     /translation="MRRKFLFFVVLLIISPTIAGNVNLRDVTANEWREDIAFLERTLV
                     KQHPDAFNKVYQEEFNRALQKLQTGLESLQWYEIYTSLTALVAMIGDGHTVIYPDTKL
                     RAFPVYPYAFNNGIYIIATDENNRELLNSRLVGVDELDVKELLDVLRPVVSHDNEWGF
                     ISALPNYFTRVEIMKALNLTGEEDSLKLKLDKDGEIFERIVLPAEGDFQWVQERAQET
                     FRSQTNDNYWYELYPDSKLLHFHYDRCKSQEGKPFIFFTCEMFSYMRRQSAEKLLLDL
                     RYNGGGNSLILEFFMLRVLLDCKYNREGRLFVAIGRETFSSAILNAISMKKRTRAIFL
                     GEPTGGSPRHYGAPRSFTLPNSGIIVHCSTTFWRTTSDTSEAMLPDVLIERSFEDYYY
                     MRDPVVEYIESLPIPE"
     gene            2663..3397
                     /locus_tag="PLD15_07170"
     CDS             2663..3397
                     /locus_tag="PLD15_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB63722.1"
                     /translation="MLLIYVFVLEPNTLMVERLKFSDTPSLKIAFFADIHIWIKKPLH
                     SLFLNELKQEKVDLILFGGDVLSPYTDMKYFAELFTELASIAPIYAVYGNWEESETAV
                     VDRIFKQAGVQVLHTRSTTVEVRGKKIGITGAPSHHYFSWTRFLPEEEFDLKILMVHA
                     PNMLEGSVDILKKYDLVLAGHTHGGQFYLPWLTEQVLKSTRGFSGRYFRGIYELGETQ
                     IYVTRGLGGWFPGRFASLPELVIIEL"
     gene            complement(3398..3898)
                     /locus_tag="PLD15_07175"
     CDS             complement(3398..3898)
                     /locus_tag="PLD15_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPB63723.1"
                     /translation="MQIRSREKGFSMIELLIVLSIIAALIALITPIAMNASQKARATE
                     IAKNMKTLADSIERIALTDGVDSSDKIKGVNSLSDVARDVNESTYHIIYYVTDQVKTY
                     DAYIVHKGDKLLEKVREVLSDASSTTWSDLESRWNSYTPVYLEDSPLEESDNIIYYKI
                     SFYIYQ"
     gene            complement(4263..4904)
                     /locus_tag="PLD15_07180"
     CDS             complement(4263..4904)
                     /locus_tag="PLD15_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HPB63724.1"
                     /translation="MFFGKKEMAIIQLFEEHLEYIGRTLENLEKVFLAILQNDETSIE
                     IYAEEVHKMESAADSKRREMENAMYQGAFLPNFRGDFLGLAESFDKVANEAENVVDQI
                     VLQNLIISDDLKGDLLKQVQLSVETYRVCREAAVNLFQELDVAEEKIKETEKLENIED
                     SHERALVKKIFEKDISLAEKRQLRELVISIGNIANLSEDCSDRMEIIVLKRRV"
     gene            complement(5123..>6795)
                     /locus_tag="PLD15_07185"
     CDS             complement(5123..>6795)
                     /locus_tag="PLD15_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB63725.1"
                     /translation="IKVRAVEVVDGMYRPYEAITGVEFGQAIAKVVFGVDEEIDYGAK
                     LAGLVYAPAELLSLKRPLRREEAAQILSNFVDSPDFQVITILATADIHGHIEPYTPSG
                     AKYPIGAMEKMAYYVNTERAMQPNLLLLDVGDAPYNTNVANLFEGEPVIKIMNMMGYD
                     AMAIGNHDFDFPFNVMERNSKLASFPFLSANTLFMEKSVEFLKEYVIKEIGGLKVAIV
                     GLTDDSSAWYTHPRNVAGITFEEQYTAAQRVLDEVAPQADLVIALAHLHTGNRILPAK
                     VSGYDLIFEGGRDVVAFPEKINGSWVISSGKHGELISKTNLNIFNGEVIGINFAHVFM
                     SQNLPQDEAVVEVVNGYVAQLDEKMDTVIGRTEVDLDGERGTVRLKESNLANAIADSL
                     REMTGTDFAIQNGGGVRASIPKGDITIKDVYTVLPFDNLVVAVEITGKQIWDVLEHGI
                     STYPAAAGQFLQVSNLEYTFDGSKPPYSRLISVTSNGVPLDMEKTYTLTANDFLTGGG
                     DKFVMFLETKKIIVTKSFLRDAFAEYVIRHGTIAPQFEGRITIINPAN"
BASE COUNT         1669 a         1636 c         1536 g         1954 t
ORIGIN      
        1 tggtgttgta gaaagtaact atatcgttgt agtacgctct tgaaagctct atcctagtct
       61 cagtgtctgc taattccttc tggagcttca aaactacctc gctggatttg agggaagggt
      121 acttctcaat cagggccagc aacctgccgc caacaccctc aaaaccgtta tatgcttcac
      181 tcctcaaaga agccagtgcc tcctgtactc cctgttcatg ccgcatcaac cccaagacgg
      241 tcgagacaag tgaggggacc aagtccttcc tccgcttcaa ctgaacttca atctgggact
      301 taccctgtct ggttctctct ctgagagatt tgagagcgtt gaaaactgtc cagaaccatc
      361 caatcgtcca ggcgaccagg tatagtccag cggctatgat ttgattcatg taggaaaaag
      421 gtatccagcc gtatcctgtc cgattagata tgaagaccgt aaagaaagct acagccaatc
      481 caccaagcgc caagagccag taattgaagc cgtaggaacg actcaccttc ttttcatcac
      541 gcattgaaat cagaaagacg ggagcttctc gatcgtaggc tatctcagcg gcaaccttat
      601 cctctctctc gcgtgcctga cccataacgt aaagcgtaga gccgttgggt attatcgtct
      661 ctgtgaaaca cctcctgtag tcggagttca ttatcgagcc ggccggaccc tttccgtaat
      721 acatgggatc agaagggcca cagtagtagt tgaggataca ctcgccatct acttttgagt
      781 cttccggtct cacctgtatt gcaccgctct cgtctctgag catgaagtga gacatctgct
      841 catcggaagc gattgtcttc cagccacttt cggttcttgt tctagtcttt gtcttgccct
      901 gcgaatcggt gtaggtttca gttactgttt tactccagtg ctcggatacg ttccatctgt
      961 aatatacaca ttcgctttcg gtcaaataac tacgaagagg tgaggggcat tcgacctttc
     1021 ccgaaacttc aactaatcca ataaaaactc cgtgagcctt cgaggtgggg gtatcgtcca
     1081 ccagccgctt ctttttcaaa gccttgaaag cgaaaaaaag cagaaacaat gaaacggccg
     1141 taccaaatac aagaacaacg gtgttgatag ccatctatat tactcccggt tattccggta
     1201 tgggaagtga ctctatatat tcaacgaccg gatcacgcat gtaataataa tcttcgaaac
     1261 tcctctctat caggacgtca ggcagcatcg cctcggaagt atcggaagta gtcctccaga
     1321 aagtcgtcga acagtgaacg atgattccac tgttggggag agtaaaactt ctgggcgctc
     1381 cgtagtgacg gggactgcca ccggtaggct cacctaaaaa aatcgccctg gtacgctttt
     1441 tcatcgatat tgcattcagg atcgccgagg aaaatgtctc tctgccgatt gcaacgaaga
     1501 gcctgccttc tctgttgtat ttgcagtcca gcagcaccct caacatgaaa aactccagaa
     1561 tcagcgaatt accgcccccg ttgtagcgga ggtcaagtaa aagcttttcg gcactctgcc
     1621 ttctcatgta cgaaaacatc tcgcaggtga agaatataaa aggttttcct tcctggctct
     1681 tgcagcggtc gtagtgaaag tggagaagtt tcgaatcggg atagagttcg taccagtagt
     1741 tgtcgtttgt ctggcttcta aaagtttcct gcgcacgctc ctgaacccac tggaaatctc
     1801 cttcggctgg caggacgatt ctttcgaaaa tctctccgtc cttatccagt ttcagcttca
     1861 ggctatcttc ctctcccgtc aaatttaggg ccttcattat ctcgactctg gtaaaataat
     1921 tcggtaatgc tgagataaag ccccattcgt tatcgtgaga aacaacaggc ctgagcacgt
     1981 ctagaagttc tttcacatcg agttcatcta caccaacgag ccttgagtta aggagttcgc
     2041 ggttgttttc atcggtagcg attatgtata tcccattatt gaaggcgtaa ggatatactg
     2101 gaaaagctct cagcttggtg tctggatata ttacagtatg accgtcccct atcatagcaa
     2161 ccagcgctgt gagagaggta tatatttcgt accactgtag agattcaagg ccggtctgga
     2221 gtttctgcaa agcccggttg aactcctcct gatagacttt gttgaaagca tcgggatgtt
     2281 gcttcaccag agtcctttct agaaaagcta tgtcctctct ccactcattc gccgtgacgt
     2341 ctctcagatt aacattcccg gcgatagttg gcgagataat gagtagcacg acaaaaaaca
     2401 gaaattttct gcgcatttgc gacctccttt cataccaatg gatattttaa cactattggc
     2461 ttctaaaata atacggtcag ctttgagagc gacttttagt tgtttggtcg catcagcaga
     2521 ttgaaagact caattttagt gtaaagaggt ttttggagga ttgtgtctcc ctggaggttt
     2581 tttgatatac aattgagtag aatatttctg tgaaatgggg attgtttatt tgaagcactg
     2641 gaagctctgt ttattattat ttgtgctctt gatttatgtt tttgttctgg agccgaatac
     2701 tctgatggtc gaaaggttga aattcagcga tacgccctcg ttgaaaatag ccttctttgc
     2761 cgatattcat atttggataa agaagcccct ccactcgctt ttccttaacg agctcaagca
     2821 ggaaaaagtc gatctaatac tttttggggg agatgtgctc tcgccttaca cagacatgaa
     2881 atatttcgcc gaacttttca cggagcttgc ttctatcgct cctatttacg ctgtctatgg
     2941 caactgggag gaatcagaga cagcggtcgt tgaccgtatt ttcaaacagg ccggtgtgca
     3001 ggttcttcac acacgttcaa ccacagtcga agttcgcgga aagaagatcg gaataaccgg
     3061 tgccccctct caccattatt tctcgtggac acgttttttg cccgaagagg aattcgatct
     3121 caagatactc atggttcacg cacccaacat gctggaaggt tcggttgata tattgaagaa
     3181 gtacgacctt gttctggcag gccacacaca tggaggtcaa ttctatctgc catggttaac
     3241 cgaacaggtt ttgaagagca caagagggtt cagcggcagg tatttcagag gtatatacga
     3301 actgggtgag acacaaatat acgtcacaag gggtctcgga ggctggttcc cggggaggtt
     3361 cgccagtctt cccgagcttg ttataatcga gctatgatta ctggtagata tagaaactga
     3421 tcttgtaata aataatattg tcgctttcct ccagcgggct atcttcaaga tagacgggcg
     3481 tgtaggaatt ccaccgactc tccagatcgg accacgtcgt agatgaagca tcactcaaca
     3541 cttccctgac cttttccagt aacttatcac ctttatgcac aatgtaagca tcgtatgtct
     3601 ttacctggtc ggttacataa taaattatgt gataggtcga ttcattgacg tctctggcaa
     3661 catcggaaag agaattgaca ccctttatct tgtcgctgct atcaacacca tcagtgagag
     3721 ctatcctctc gatcgaatcg gccagagttt tcatgttttt tgcgatttcg gttgcccttg
     3781 ctttctgaga ggcattcatg gctatcggtg tgataagggc gataagtgcc gctattattg
     3841 agagtactat aagtagctca atcatcgaga agcctttttc tcttgaacgg atctgcacgc
     3901 ttttcaaatc aggtcccctc ccggctttaa gacaaactgt gcaatttcat gccaagtatt
     3961 tttccgagcg agaattcatt caaacgattc tgcaagcctt acatacggat tttctggaaa
     4021 taaaactgaa gcgggtttcc cggttgaggg gatttccgtg aaatcaattt cgtgatgaat
     4081 acctgtggga agtggtattt gattcaattc atctatttgg attcacaatt ttagctgaag
     4141 ttcgatggtg tttgtttcag attctcaaga atactaccgt aggccgaata caggctccaa
     4201 aagtagcgaa gttaatatct gtcacacgtt gcatgtcctg aaagtcttta gaggtaaaaa
     4261 ggctacactc ttcgcttcag aacaattatc tccatcctgt cagaacaatc ctcggagagg
     4321 tttgcgatgt tgcctataga gatgacaagt tctctgagct gtcgcttttc agccagagag
     4381 atgtcttttt cgaagatctt tttcacgagt gctctctcgt gactgtcttc gatgttctcc
     4441 agtttttcgg tttctttgat cttctcctcg gcgacatcca attcctggaa aaggttgaca
     4501 gcggcttcgc ggcagactct gtaagtttcc accgaaagct gtacctgttt caacaggtct
     4561 cctttgagat cgtccgagat tatgagattc tgcagaacga tctgatcgac aacgttctcg
     4621 gcttcgttgg caactttatc gaaagactcc gccagcccca gaaaatctcc cctgaagttc
     4681 ggtagaaaag caccttggta catcgcattt tccatttcgc gtctcttact gtccgcagcc
     4741 gattccatct tgtgaacttc ttcggcgtag atttctatcg atgtttcgtc gttttgcagg
     4801 atggctaaaa agactttttc gaggttttcg agagttcttc cgatatattc cagatgctct
     4861 tcaaacagct gtattatggc catttctttc ttaccgaaga acataagagg tccacctcca
     4921 ggcttgagga tacttatgaa tccattagag ccgtttcctc tcataaaaat cacatccgtc
     4981 actgagattt cggttccggc aacaagaatg gctactaata gattttatta cttatatatt
     5041 atgacacttt tatgtgattg tgttctcagt tatcgaaaaa gcggagaggt aaacctctcc
     5101 gcttcgttga aaagcccgct cttcaattgg cagggttgat tatggttatt ctcccttcga
     5161 actgcggagc tatggttcca tgccttatta cgtactcggc aaaggcgtct ctgaggaatg
     5221 acttcgtcac tattattttc ttcgtttcaa ggaacatgac gaacttgtct ccacccccgg
     5281 tgagaaagtc attggccgtt agcgtgtagg ttttctccat atcaagaggg accccgttcg
     5341 aagttacaga gatcagcctg ctgtatggag gtttcgaccc atcgaaggta tattcgaggt
     5401 tcgagacctg caggaactgg ccggcggcag ccggataggt agaaataccg tgttcgagaa
     5461 cgtcccatat ctgtttacct gtgatttcga ctgcaaccac caggttatcg aacgggagta
     5521 cggtataaac gtccttaatc gttatatcgc ccttaggtat acttgccctt accccgccac
     5581 cgttctgtat agcgaagtcg gtgccggtca tctcccttaa gctatcggct atggcgttgg
     5641 ccagattaga ttccttgagc ctcaccgtgc ctctctctcc atccaggtca acttccgttc
     5701 tgccaatgac ggtgtccatt ttttcatcga gctgtgccac atagccgttc acgacctcaa
     5761 cgacggcttc gtcctgagga aggttctggc tcatgaagac gtgtgcgaag ttgataccta
     5821 tgacttcgcc gttgaagata ttcagatttg tctttgaaat aagctctccg tgttttccag
     5881 aagaaatgac ccagctaccg ttaatctttt cggggaaggc tacgacgtct ctaccgcctt
     5941 cgaatattag atcgtaaccc gaaacctttg caggaaggat cctgttcccc gtgtggagat
     6001 gtgccagggc gatcaccaga tcggcctgtg gagctacttc atccagtact ctctgggcgg
     6061 ccgtatattg ttcctcgaag gttattccgg cgacgtttct tgggtgggtg taccaggcgc
     6121 tgctatcgtc tgtaagcccg actatggcga ctttgagacc tccaatctcc ttgataacat
     6181 actcctttag aaattccaca gacttttcca taaagagagt attggcactc aggaagggaa
     6241 aactagcgag tttgctgttt ctctccatca cgttgaaagg gaagtcgaag tcgtgatttc
     6301 cgatcgccat tgcgtcgtac cccatcatgt tcattatctt aattaccggt tcaccctcga
     6361 agaggttggc aacgttagtg ttatacggcg catctcctac atccagaagc agaaggttag
     6421 gctgcatcgc acgttctgtg ttgacatagt aagccatttt ctccatcgca cctatcgggt
     6481 atttcgcacc actgggtgtg taaggctcta tatgcccgtg aatgtcggcg gtggcgagta
     6541 tggtgataac ctggaagtcg gggctatcaa cgaagttaga gagtatttgt gcagcctctt
     6601 cccttctcag cggccttttc agggatagta actcggcggg agcatagacc agcccggcga
     6661 gtttggcccc gtagtcgatc tcttcgtcaa caccgaagac cactttcgct atcgcctgac
     6721 cgaattcgac accggtgatc gcttcgtagg gacggtacat accgtcaact acctctacgg
     6781 ctcgaacctt tatca
//
LOCUS       DAOOLY010000123         6760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13413_length_6760_cov_4.380015, whole genome shotgun sequence.
ACCESSION   DAOOLY010000123 DAOOLY010000000
VERSION     DAOOLY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13413_length_6760_cov_4.380015"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="PLD15_07190"
     CDS             complement(<1..1660)
                     /locus_tag="PLD15_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPB63726.1"
                     /translation="MLKSNSGESTNVVGKMVKRVDALEKVNGCARFAADIAFDGQIYC
                     ALVIAEKPHGILRSIDVSLASKMEGVEAVFTWRDIPGDNQLGEAIKDMPCLVKEGEKI
                     RYWGDVVAVVAARDRISAENAAKSVRLDIEDLEPVLSIEEALEDRIKIHGGSNVKVTK
                     LIRKGDPETVIKNSKEVFEGEFYAHYQEQAYMEPQGITVVPDLDYGYTVYGTMQCPYY
                     VQSAVAHVLGLPMNRIRVIQTETGGAFGGKEDVPSYVASYAAVAAFNLKRPARLIYSR
                     EIDIQTTSKRHPIKSFYRVAMDGKKIKAIEVMAYMDMGAYATLTPIVMFRTLVHAAGA
                     YDIENVNVDVYGVYTNKVPPGAFRGFGSPQVLFAVESMIDDIAWKLRIDPMELREENT
                     LRPGSRTSTDHLIVESVGAPETLARVKESSHWKDLVKECIEFNRANEDKKRGAGLSHI
                     FYGVSLGAGGQALDKSGAHVLINRDGSVEVRIGGTEMGQGAKTVIAMMAAEELGQRME
                     RILVHQPDTAFVPDSGPTVASRTTIYSGNAVRRACITLKGKLISV"
     gene            complement(1653..3395)
                     /gene="ade"
                     /locus_tag="PLD15_07195"
     CDS             complement(1653..3395)
                     /gene="ade"
                     /locus_tag="PLD15_07195"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPB63727.1"
                     /translation="MNIIDIIPVSRGDSPADVLLKNCRIVNVFSGEIEKGNIALFRKR
                     IAGIGDYGEGREVIDLEGAYIVPGLIDAHLHIESSMVSPVEFAKTILARGTTTAIADP
                     HEIANVMGLSGIEYMIRSTEGIPVNLYIMIPSAVPASSMETSGARISVMDMIGFVEKF
                     QSRVLGLGEVMNFPDIINGDRDSIAKIELIRHKYKKIDGHIPSVLGKPLNAYISAFVR
                     SEHECTFVEEAREKLSRGMQILMREGSVERNLVPLLPLINDRTYPFVSFCTDDKHPVD
                     IIREGHIDHNVRLAIEHGIDPIIAIRAATINTARHYNLRSMGAIAPGYKADLVVIDNL
                     KDFNPIMVFKDSKIVARNGRLVTEIISQNFPQEKVNTFKCQKINESDLEIRAEGRLVR
                     AIELLGSEVLTGGCVMGAKIENGKAVGDLSKDLLKVAAICRYCEGKSMSVAFATGSGL
                     KKGAVATSVGHDSHNLGVLGTNDPDMVAAANRVMEMGGGLVAVIDGKIISELPLRIAG
                     LMSDMTSKEVAERLIELKDATRTMGSKLPDLFMTLSFMQLSVIPKLKLTNLGLVDVEK
                     NDFVSLFVEEGQDA"
     gene            complement(3465..5411)
                     /gene="thrS"
                     /locus_tag="PLD15_07200"
     CDS             complement(3465..5411)
                     /gene="thrS"
                     /locus_tag="PLD15_07200"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPB63728.1"
                     /translation="MGIEIKLPDGSKKQYEKGARPLDIAMDISEGLAKRAFGAIVNDE
                     YWDLGRPLETSCDFRLVLDKDSEAPVFFRHTMAHIMAQAVMRLYGEDRVKLAIGPTIE
                     NGFYYDIDLDDVRLTEEDLPKIEAEMEKIIKEDLPIERFELPVSEAVILMNNQLQPYK
                     VELIEDLVKDYGTEFVTFYRQGDFTDLCRGPHLPSTGKVKFFKLTSVSGAYWRGDEKN
                     KMLQRIYGTAFSKKADLEEYLAKIEEARKRDHRKLGPALGLFTINYDYAPGMPIFLPK
                     GTDMLNELLKFSREKHIEDGYREVSTPQIMSDSLWRTSGHWDHYSDNMYFTEKEEQQF
                     AVKPMNCPGHILAYKSSPVSYRDLPMKIFEFGKVHRYERSGVLHGLFRVRGFVQDDAH
                     VFCTREQIQQEVIGVIDFVKKIYEPFDFQYRAELSTRPENFMGEIELWDIATKALRDS
                     LKAKKMDYIVNEGDGAFYGPKIDYHIKDSLGREWQCATIQLDFMMPERFGIKYIGPDN
                     NEYQPVMIHRAIFGSTERFMGILIEHYAGAFPTWLAPVQVKVIPIADRHTDYARSVVV
                     SLRGKGFRAEVDDRQKSTNYKIREAQLNKIPYMLIVGDREMQNGQVSVRLRSEKDLGS
                     IDLEEFIHKLTEEVKDRKGKSIFE"
     gene            complement(5425..5802)
                     /locus_tag="PLD15_07205"
     CDS             complement(5425..5802)
                     /locus_tag="PLD15_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPB63729.1"
                     /translation="MLRVISTDKAPAAVGPYSQAVAAGGLLFVSGQIPLNPLTGELAT
                     EFEAACKQSLENVLQLVTAGGSSLKNVVKVNIYIRDMGKFAILNEIYSSYFKDHKPAR
                     AVVEVSNLPKNAPVEIEAVAVIP"
     gene            5919..6650
                     /locus_tag="PLD15_07210"
     CDS             5919..6650
                     /locus_tag="PLD15_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63730.1"
                     /translation="MIREIMASSALTRSRIPVASYTINPFVGCTVGCKYCFARFIGAF
                     KYTGGLWGRDVGVKRNIVELLRREVNSLGKATVFLSSTCDPYQHIEEKYKLTRELLVT
                     LISHRIPIFAMSKSALIRRDVDLFKLAGEEARVLISITTDREDVRKLLEPGSSTFEER
                     LETIDFLRKNGVDAGAFVGPVLPMNAERVSEELARRVGTVHLDALNYISQVRQLYLKM
                     GWERWLYREKFQEVQAVFERRLTVE"
BASE COUNT         1448 a         1846 c         1666 g         1800 t
ORIGIN      
        1 aaacggaaat caatttccct ttcaaggtga tacaggcccg tctgacggcg ttgcctgaat
       61 agatggtcgt tctggaagcg actgtcggtc cgctgtccgg cacgaaagcc gtgtccggct
      121 ggtgaacgag tatcctctcc attctttgac ccaactcctc ggctgccatc atggctatga
      181 cggttttggc gccctggccc atctccgttc cacctatcct gacctcgacc gaaccgtccc
      241 tgtttatgag cacgtgcgcg ccggacttgt cgagcgcctg acccccggcc cctaaactca
      301 ctccgtagaa tatatgagag agaccggcac cgcgtttctt gtcttcgttg gctctgttga
      361 attcgataca ctccttcacc aggtccttcc agtgcgacga ctccttaact ctcgcgagcg
      421 tttcgggagc cccgacgctt tcgacgatca ggtgatctgt cgaggttctc gaaccgggtc
      481 gtaacgtgtt ttcctcccgc aattccatcg gatcgatacg cagcttccag gcaatatcgt
      541 cgatcatact ctcgaccgcg aagagaacct gcggcgagcc gaaacctctg aaggctccgg
      601 gcggaacctt gttggtgtaa acgccataga catcgacgtt aacgttttcg atatcgtacg
      661 cacccgcggc atgcaccagc gttctgaaca tcactatggg ggtaagcgtc gcgtaggctc
      721 ccatgtccat gtatgccatg acttcgatgg cctttatctt ctttccgtcc atcgccacgc
      781 ggtagaagga ttttatcggg tgacgtttgg aggtagtctg gatatctatc tcgcgagaat
      841 agatgagcct tgccggcctc ttaagattga aggcggccac cgccgcgtac gaggcgacgt
      901 acgagggcac atcctccttg cccccaaagg ctcctcccgt ctcggtctgt atgacacgta
      961 ttctgttcat gggcagtccc agaacgtgag cgacggcgct ctgaacgtaa tagggacact
     1021 gcatggtgcc gtaaactgta taaccgtagt cgaggtcggg caccacggtt ataccctgtg
     1081 gttccatata ggcctgttcc tggtagtggg cgtagaattc gccttcgaat acttctttcg
     1141 aattcttaat aacggtttcg ggatcgccct ttcttatcag tttagttact ttcacgttcg
     1201 aaccgccgtg tatcttgatt ctatcctcta gcgcctcttc tatagatagt acgggttcga
     1261 gatcttctat atcgagtctc acgcttttcg ccgcgttctc ggccgagata cggtctctgg
     1321 cggcgacgac ggccaccaca tcgccccagt aacgtatctt ctcgccttct ttgacgaggc
     1381 agggcatatc ctttatcgct tcgccgagct gattatcgcc cgggatatcc ctccaggtga
     1441 agacagcttc taccccctcc atcttggagg cgagagatac atctatcgat cggagaatgc
     1501 cgtgcggctt ttcggcgatc acaagcgcgc agtagatctg accgtcgaag gctatgtcgg
     1561 ccgcgaacct ggcacaaccg tttaccttct ccagggcgtc gactcttttc accatctttc
     1621 ctacaacatt cgtcgattct ccggaattac ttttaagcat cttgcccctc ctcgacaaac
     1681 agggatacga aatcgttctt ttccacatcg accagtccga ggttggtcag cttcaacttc
     1741 gggataacgg agagctgcat gaaagagaga gtcataaaga gatcgggtaa tttgctcccc
     1801 attgtcctgg tggcatcctt caattcgatg aggcgttcgg cgacctcctt gctggtcata
     1861 tcggacatca gaccggcgat ccgcaagggc aattccgaga tgatcttgcc gtctatcacc
     1921 gccaccagac caccacccat ctccataact ctgttggcgg cggcaaccat gtccggatca
     1981 tttgtaccga gaacgcccag gttatgtgaa tcgtggccga ccgaggtcgc caccgcgcct
     2041 tttttgagac ccgagccggt cgcgaaggcc accgacatcg attttccttc gcagtagcgg
     2101 cagatcgcgg caactttcaa gagatcttta gatagatcgc cgacggcctt gccgttttcg
     2161 atcttcgcac ccatcacaca gccaccggtg aggacctcgc tcccgagcag ttcaatggct
     2221 ctcaccagcc ttccttcggc ccgtatttca aggtcgctct catttatctt ctgacacttg
     2281 aaggtattca ctttctcttg cgggaagttc tgagagatta tctcggtcac gagccggccg
     2341 tttctggcca cgatcttcga atctttgaaa accatgatgg gattgaaatc ttttagattg
     2401 tctatcacga ccaggtcggc cttatacccc ggcgctatgg cgcccatcga tcggaggtta
     2461 taatgccgcg ctgtgtttat cgtcgccgcc cttatggcga ttattggatc tataccgtgc
     2521 tctatcgcta gcctgacgtt gtggtcgatg tgtccctcgc gaattatatc taccgggtgt
     2581 ttatcgtcgg tacagaagga tacgaagggg taagtcctat cgtttataag aggcagcaac
     2641 ggtacgaggt ttctctcgac gctcccctct ctcataagta tctgcattcc cctggagagt
     2701 ttttcgcgcg cctcctcgac gaaagtacat tcgtgctcgg atctgacgaa agcgcttata
     2761 tatgcgttca ggggttttcc gagaacgctg gggatgtgac cgtcgatctt tttgtatttg
     2821 tggcgtatca gctctatctt ggctatggaa tcgcgatcgc cgtttatgat atccgggaag
     2881 ttcatcacct ctcccaaacc cagtacacgg ctctggaact tctctacgaa tccaatcata
     2941 tccatcacgc tgatcctggc acccgaggtc tccatcgaag aggccggaac ggccgaaggg
     3001 atcatgatgt acagattcac gggtatgcct tctgtcgagc gaatcatgta ctcgatgccg
     3061 gacagaccca tcacgttcgc tatctcgtgc ggatcggcta tcgcagtcgt cgtacctctg
     3121 gccagaatcg ttttggcgaa ctcaaccggc gagaccatcg aggactctat atgaaggtgg
     3181 gcatctatca accccggtac gatgtaggca ccctcgaggt cgatcacctc cctaccttcg
     3241 ccgtagtcgc cgatcccggc tattctcttt ctgaaaagcg ctatattgcc tttctctatc
     3301 tcgccgctga atacattcac gattctgcag ttcttgagaa gtacatccgc agggctgtcg
     3361 cctctggaaa cgggtattat gtctatgatg ttcataagag atgcctcctt tctgccttag
     3421 ccggaaagaa ataaaagccc ccgctacgca ggggcttaaa acaatcattc gaagatactt
     3481 ttgccttttc tatctttcac ttcttcggtg agtttgtgta tgaactcctc gagatcaata
     3541 cttcccagat ccttttcact tctcaacctg acggagacct gtccgttctg catctccctg
     3601 tctccgacga tcagcatgta cggaattttg tttagctggg cctctctgat cttgtaattg
     3661 gtagatttct gcctgtcgtc gacctcggcc ctgaagcctt tgccacgaag cgagacaacc
     3721 acagaacgtg cgtaatcggt gtgcctgtcg gcgatcggga tcactttcac ctgtaccggc
     3781 gccagccacg tcgggaaggc cccggcgtaa tgctcgatca agatacccat gaacctctcc
     3841 gtcgaaccga agattgccct gtggatcatt accggctggt attcgttgtt gtccggaccg
     3901 atgtacttta ttccgaatcg ctcgggcatc atgaagtcga gctgaatcgt cgcacattgc
     3961 cactcccttc ccagagagtc ctttatatgg taatcgatct tggggccgta gaaggcaccg
     4021 tcaccttcgt tgacgatgta gtccatcttc tttgctttta gagaatctct gagcgccttc
     4081 gtcgctatat cccagagctc gatctctccc atgaagtttt ccggtctcgt cgaaagctcg
     4141 gccctgtact gaaagtcgaa tggctcgtaa atcttcttca cgaaatcgat gacgccgatc
     4201 acttcctgct gtatctgctc gcgtgtacag aagacgtggg cgtcgtcctg cacaaagccc
     4261 ctgactctga aaagtccgtg tagaacgccc gaccgctcgt atctgtgaac ttttccgaat
     4321 tcgaagatct tcatcggaag atccctgtag ctcaccggag aggatttata agcgaggatg
     4381 tgaccgggac agttcatggg cttgacggcg aactgctgtt cttctttttc cgtgaaatac
     4441 atattgtcgc tgtaatggtc ccagtggccg gatgtacgcc agagtgagtc ggacattatc
     4501 tggggcgtac ttacttccct gtagccgtcc tcaatatgct tttctctgga gaatttgagc
     4561 agttcgttga gcatgtctgt gcccttcggc aggaagatgg gcatgccggg cgcgtagtcg
     4621 taattgatcg tgaacagacc gagtgctgga ccgagctttc tgtgatccct cttcctggcc
     4681 tcttcgatct tcgccaggta ttcttccagg tcggctttct tggagaaggc agtgccatat
     4741 atcctctgca acatcttgtt cttctcgtct ccacgccagt aagcgccaga aacggaagtg
     4801 agtttgaaga acttcacctt acccgtagag ggtagatgag gtccccggca gaggtctgtg
     4861 aaatcaccct ggcggtagaa cgttacgaac tccgttccgt aatctttcac taggtcttcg
     4921 atgagttcga ccttgtacgg ttgtaactgg ttgttcataa gtattacagc ttcggaaacc
     4981 ggcaactcga acctctcgat cggtagatct tccttgatga tcttctccat ttcggcctcg
     5041 atcttcggca ggtcttcctc tgtaagtctc acatcgtcga gatcgatgtc gtagtagaag
     5101 ccattctcta tggtaggacc gattgccagt tttactctgt cctcgccgta gagtctcatc
     5161 acggcctggg ccatgatgtg cgccatcgta tgcctgaaga aaacgggggc ctcactgtcc
     5221 ttgtccagca ccagcctgaa atcgcagctt gtctccagag gccttccaag gtcccagtac
     5281 tcgtcgttga ctatcgctcc gaaagccctt ttggcgaggc cttcagatat gtccattgct
     5341 atatccaggg gtcttgcacc cttttcgtac tgttttttcg aaccatcggg taactttatt
     5401 tctataccca ttttttcctc ctgattaagg tatcactgca acggcttcga tctctacagg
     5461 ggcgtttttc gggagattgc ttacctccac cacggccctc gcgggcttat ggtctttgaa
     5521 gtaagaggag tatatctcgt ttagaatcgc gaatttgccc atgtctctga tatagatgtt
     5581 gaccttaacc acatttttca gactcgaccc gccggcagtg accaattgaa gtacgttttc
     5641 tagcgactgc ttacacgcag cttcgaactc tgtcgccagc tcaccggtca acgggttgag
     5701 ggggatctgt ccggaaacaa agagaagacc tccagcggca acggcctgag aatacgggcc
     5761 gactgctgcc ggcgctttgt cagtactgat aaccctcaac acactaacac ctcccatttt
     5821 ggtttattat atcactgact gctacgtgat gctgcgcatt atctactata tgagaaaaaa
     5881 cgtgtggaag atatacgaat gtattgtaga atttcgttat gataagagag ataatggcct
     5941 cttcggcctt gaccagatcg cgaatacctg tcgccagcta tacgataaat ccctttgtcg
     6001 gttgcacagt gggctgcaag tactgtttcg cgcgcttcat cggggccttc aaatataccg
     6061 gaggtctctg ggggcgggac gtaggagtga agaggaacat tgtggagctc ctgagacgcg
     6121 aggttaattc tctgggcaaa gccacggtct ttctcagcag cacctgcgat ccgtaccagc
     6181 atatcgaaga gaagtacaaa ttgaccagag aactgctggt tacactcatt tctcacagga
     6241 taccgatctt cgcgatgtcc aaatccgcac ttataaggag agatgtcgat cttttcaaac
     6301 tggctggcga agaggctcga gtgttgataa gtatcaccac agatcgggag gacgtgagaa
     6361 aactgctcga accgggatcg tcaaccttcg aagagaggct ggagaccatc gatttcttga
     6421 gaaaaaacgg tgtcgacgcg ggagccttcg tcgggccggt tctcccgatg aacgcggagc
     6481 gcgtctccga agagctggcc agaagagtcg gaactgttca tctagacgcg ctgaactata
     6541 tttcgcaggt gagacagctt taccttaaaa tgggctggga gagatggctt tacagagaga
     6601 agttccaaga agtgcaagcc gtgtttgaaa gaaggctgac ggttgaatga acattgtcga
     6661 ataatggctc ccaataattt tagcgactta caaagcacca tgtctgtcag cgttttttaa
     6721 gtttctggac taatttctta ccttgaaccg ctgcggtggc
//
LOCUS       DAOOLY010000124         6706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13568_length_6706_cov_3.971283, whole genome shotgun sequence.
ACCESSION   DAOOLY010000124 DAOOLY010000000
VERSION     DAOOLY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13568_length_6706_cov_3.971283"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLD15_07215"
     CDS             <1..192
                     /locus_tag="PLD15_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB63731.1"
                     /translation="VAYGIASLVAEASEKLYALKRLVEKYSPDFEKESEDVSKRSLNT
                     TCIIRIDIQHISCKGHIEV"
     gene            272..751
                     /locus_tag="PLD15_07220"
     CDS             272..751
                     /locus_tag="PLD15_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63732.1"
                     /translation="MNKETLFRIAIGSIMVLFGLLIILVPSFTVNLWLLFVWAPAIFM
                     EYRAVTGGDKGLFVPGGILLVISLILTLNVIFPNFIAAGGWALFIMAPAVGLFQLFIA
                     EEKKDSGLLFAASLLAAISVFFVLMNVAPKAMGIFVGLVLLAIGVFVVFKNIRSNKR"
     gene            762..1871
                     /locus_tag="PLD15_07225"
     CDS             762..1871
                     /locus_tag="PLD15_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPB63733.1"
                     /translation="MKAMILAAGAGTRLKPLTNKIPKPMLPIVEKPVIEFIIELLARH
                     EIKEIMINISHLGWVMQNYLKSGYRYGVKIGYSFEGHFEKGQLVADPIGSAGGMRRIQ
                     DGSAFFDETFIVVCGDAVIDFDLKRALDFHNSRGSIATILTKEVPNEQVSNYGIVVSD
                     SQGRVGSFQEKPPLEKAGSNMANTGIYIFEPAVFKYIPSGTFYDIGSQLLPDLVKRKE
                     SVYSFTAEMEWFDIGRNSDYLSILGQALLGKIKGFKPSGKEITAGLWKGISSVVEKTT
                     RIITPVFIGSNTRIEKNVRLEGPTMIGSNCEICEGVDLSNVFVGDYTRIKTGFKGKNI
                     LITPDFFIRSDGSTGSISEGPYAEFISDTRNVDIR"
     gene            1951..3141
                     /locus_tag="PLD15_07230"
     CDS             1951..3141
                     /locus_tag="PLD15_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63734.1"
                     /translation="MLKISRLSSTRDVKDFISLPTKIYKDYPLWVPPFRHEMKAIMRG
                     KGSALFANGPHEFFLARENGSVVGRIAVGIEQTMNREKSVDHAYFTLFESIDSKPVAD
                     ALLKTAESWAKNRGMKFLKGPVSPTNGDDFRGLLVDNFEDTPAILMPYNPPYYIDIFK
                     EYKVYLKYLAFNYNLENVISEREIKGTEIAMRRYNFYVEEANFRHIKDLARDIHRVTV
                     EAMPDWEEDVIKPTFEEIYQAARTLKLVADSRMVIIARSEGRPIGYFVAFPDFSPIVR
                     EIGGKLFPFGWYKFLTKRKTIERVRAAILFVVPEFRNRGVPSAMFVKAYNNVREMGYK
                     KIEGSSVSWMNSTMIANALRAGGDEYKHYIVFGKSLIKRPLSLREIYGYAADRFISKE
                     RVFA"
     gene            complement(3113..4429)
                     /locus_tag="PLD15_07235"
     CDS             complement(3113..4429)
                     /locus_tag="PLD15_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB63735.1"
                     /translation="MKRFLLLAIFCTLFLSSLLTVAVVFSGGGGRGAYQVGVYGALLD
                     LDIEIEGIFGASVGAINAAGIISEGYEATRDFWYGMDYTQLMKASPELYDLLQGKFFS
                     LNTLELASALRLLTAKGGIDVSPLREKLQKLISEEKIRKSRMDFGVVAYSLSDVRPFV
                     IYKENCPKGLLVDYVLASANFPAFKREEIGNELFIDGGVYSNIPLFMARERGFKEVVA
                     VDVMGLRIGDSLAYVNLFNEGMEVTLLEPSQQYGTVMTFDGEISVKYAICGYLDAMKT
                     FGKLVGDRYFIFGDGDPLADRFLCLDLVSRCEALALVGLEAVEKAPAEYHYYRQLIPW
                     LETVMGCELTSPSLTVMKILEVLAGFLGIDRLWPYTPGLILEEVKRSWDPPLYIEERS
                     IQFLTKYRKLLLLVAYVSEKSPYSGVESPDFTEFLEKFRQRLALSK"
     gene            4596..6332
                     /locus_tag="PLD15_07240"
     CDS             4596..6332
                     /locus_tag="PLD15_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63736.1"
                     /translation="MQLIRYFLKQNWYRYLIGILFLISVDTLMLITPRIMGSIVDELR
                     SGVQDMEYIGLLILAVIGVAFGLFITRLFWRVFIMGSARRFEYYARKTLFQKLLDMPP
                     TFYDKTRVGDLMARFTNDVNAVRMAMGPAIVMTIDSIFLISVTIIAMGSFTSWELTWI
                     AIIPLPLLAVVSTFFGRIIHKRFKKVQAAFSNLTDTVEESVSGARLIKSYGIEGLRND
                     TLSEKSDSYVQENIKLIKVWGMFFPLIQMLSMLGSVLATLFGGQMVVLGQLTLGEYVT
                     FTSYLGMLIWPMTAFGWVINMVQRGRASYKRLLEVLEEKNNIVVEDPVSLEDFNGHIS
                     LKRLTFTYPTGNRPVLHDIDFEVIPGSKVAIVGTTGSGKSTLAKLIARLYPVEKGMLF
                     IDGYDINRLPPEVIRERIAYVPQETFLFSETIRNNIRFGNMKLTDSEVEKYAAIASIH
                     EDILGFPKGYDTLVGERGVTLSGGQKQRVAIARALIKQTPIIVLDDCLSAVDAETEKK
                     ILDSLKDSQGKRSIVVVSHRLKAVVDADTIYVMHEGKIVERGTHHELVENGGLYQRMY
                     ERQMIEEKLKED"
     gene            6335..>6706
                     /locus_tag="PLD15_07245"
     CDS             6335..>6706
                     /locus_tag="PLD15_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63737.1"
                     /translation="MAQNVYSEAEEKLKVEDWKVIFRLWKYTKPYLPILILALVLIGA
                     STGLDLLPPLLSKMVIDDYMNNEYTLVVIHEGDSVRFIDTEEGTFSLKQSESGDYVVT
                     DGVLQYPITREQLRELRTEDLN"
BASE COUNT         1863 a         1527 c         1650 g         1666 t
ORIGIN      
        1 gtggcttatg gaatcgcctc gttggtggcc gaagcttcag agaaactgta cgctctgaaa
       61 agactcgttg aaaagtactc acccgatttc gaaaaagaaa gtgaagacgt atccaagagg
      121 tctttgaata cgacctgtat aatcaggatc gatatacagc atatctcttg caaaggacat
      181 attgaagtat agtgagtgtt tttatgcgtt tttgtgtgcc gcttcggact tgatataatt
      241 ccgttgatcg aaatgattgg aggcgattgt tgtgaacaaa gaaacgctgt tcagaatagc
      301 tataggttca atcatggtgc tgttcggttt gctgataata ctcgtacctt ccttcactgt
      361 aaatctatgg ttgctcttcg tatgggcacc ggctattttt atggagtaca gggccgttac
      421 cggaggagat aagggacttt tcgtacctgg aggcatatta ctggtcattt cgttgatatt
      481 gactctaaat gtgatcttcc cgaatttcat tgccgccggt ggctgggctc tgttcataat
      541 ggcgcctgca gttggactct tccagctttt cattgccgag gagaaaaaag attcaggtct
      601 actgttcgcc gcttcccttc tagcagccat ttccgtgttt ttcgtgttaa tgaatgtagc
      661 cccaaaagcc atgggaatct tcgttggtct ggtgcttctg gcaattggag tgttcgttgt
      721 ttttaaaaat atcagatcga acaaaagata ggaggtaatc aatgaaagcc atgatcctgg
      781 ccgcaggtgc cggaacacga ttgaaaccgc tcaccaacaa gattccaaaa ccgatgcttc
      841 cgatagtcga gaaacctgta atcgagttca taatagagct tctcgcccgt catgaaatca
      901 aggagatcat gataaatatt tcacatctcg gctgggttat gcagaactac ctcaaaagcg
      961 gttacaggta tggagtgaag ataggatact ctttcgaggg ccatttcgag aaaggtcaac
     1021 tcgtggccga tccgatcggc tcggccgggg gtatgcgaag aatacaggac ggttctgctt
     1081 tcttcgatga aaccttcatc gtggtctgtg gggatgcggt catagatttc gacttaaaaa
     1141 gagctctcga ttttcataac tccagaggtt cgatcgcaac tatcctgacc aaagaagttc
     1201 cgaacgagca agtctccaat tacggaatag tggtgagcga ttcgcaaggc cgtgtaggtt
     1261 cctttcagga gaagccgcct ctggaaaagg ccggatctaa tatggccaat acaggcatct
     1321 acatctttga accggccgtt ttcaagtata ttccttccgg aacgttttat gacataggca
     1381 gccagcttct cccggatctc gtgaagagaa aagagtctgt atattctttc acggccgaaa
     1441 tggaatggtt cgatatcggc aggaattccg attacctgtc gatattgggt caggctcttc
     1501 tgggaaagat caagggattc aagccgtcgg ggaaggagat tactgcgggg ttatggaagg
     1561 gaatcagctc ggtagtcgag aaaacgacca gaataatcac acctgttttc ataggttcca
     1621 ataccagaat cgaaaagaac gtgagactcg aagggccaac aatgataggt tccaactgtg
     1681 aaatctgcga gggggtcgat ctcagcaacg tctttgttgg agactacacg aggataaaga
     1741 cgggattcaa aggtaaaaac atactcatca ctcccgactt tttcataagg tcggacggct
     1801 caaccggctc gatttcggaa ggtccttacg cggaattcat ttccgatacc agaaatgtgg
     1861 atatccgttg atctcttcga aaaaacgctc cagtacgcag tgtgttagaa tctgaaagtg
     1921 ttgccagtct ttaaattgga gtgtcgatcg atgttgaaga tatccagatt atccagtacc
     1981 agagacgtta aggatttcat ttctctgccc acgaagattt acaaggacta ccccctgtgg
     2041 gtgccaccgt tcaggcatga gatgaaagct atcatgagag gcaagggatc ggccctgttt
     2101 gcgaacggcc cacacgaatt tttccttgca agagaaaacg ggagtgttgt cggcaggatc
     2161 gccgttggca tagaacagac gatgaacaga gaaaagagtg tagatcacgc ttattttaca
     2221 ctcttcgagt cgatagactc taaaccagtg gccgatgcgc ttttgaaaac ggcagaaagc
     2281 tgggcaaaga acagaggaat gaagttcctc aagggtcctg tatcaccaac caacggtgac
     2341 gactttcggg ggttactggt cgataacttc gaagatacac cggcgatatt gatgccttac
     2401 aatccgcctt attatataga tatcttcaaa gagtacaaag tgtatctcaa gtatctcgct
     2461 ttcaactaca atcttgaaaa cgtgatttcc gaaagagaaa tcaagggaac cgagatagcc
     2521 atgcgacgtt acaacttcta tgtggaagag gccaacttca gacacatcaa agatcttgcg
     2581 cgggatattc acagggtaac agtcgaggca atgccggact gggaggaaga cgtaatcaag
     2641 cccacattcg aggaaatata tcaggcggcc aggacgctta agctagttgc cgacagtaga
     2701 atggttatca tcgcaagatc tgaaggccga ccgataggtt atttcgtagc gtttcctgac
     2761 ttcagcccga tagtgagaga gatcggtggt aaactatttc ccttcggctg gtacaaattc
     2821 cttacaaaga gaaagaccat agaacgggtg cgtgcggcca tactctttgt cgttcccgag
     2881 ttcaggaaca ggggagttcc ttctgcgatg ttcgtgaagg cttacaacaa cgtgagagag
     2941 atggggtaca aaaaaataga aggttcatcg gttagctgga tgaactcgac gatgatcgcc
     3001 aacgccctca gggccggtgg agacgagtat aagcattata tagtcttcgg aaaaagcctg
     3061 atcaaaaggc cgctgagtct cagagagata tacggttacg ccgcggatcg tttcatttcg
     3121 aaagagcgag tctttgcctg aacttttcca aaaactccgt gaaatcaggc gactcgacac
     3181 cgctgtatgg agatttctcg gacacatagg caactaaaag aagtagtttc cggtattttg
     3241 tgaggaactg gatggatcgc tcctctatgt agagaggcgg atcccaggag cgcttcactt
     3301 cctccaggat caaccctggc gtgtaaggcc acaatctatc gatcccgaga aaaccggcca
     3361 gcacctcaag tatcttcata acggtcagcg agggggatgt taactcgcac cccataaccg
     3421 tctccagcca gggaattaac tgccgataat aatgatactc tgccggagcc ttttctaccg
     3481 cttcaagacc gacgagcgcc agcgcttcgc accttgacac aagatcaagg cataaaaaac
     3541 gatcggcaag cggatcaccg tctccaaaaa tgaagtatct atcgcctaca agctttccga
     3601 aggttttcat agcgtcaaga taaccacaga tcgcgtactt cacggagatt tcaccgtcga
     3661 aagtcataac ggtgccgtac tgctgtgacg gttccaggag agtcacttcc attccctcgt
     3721 tgaagagatt gacgtatgca agagaatcac cgattctcaa tcccatcaca tcgacggcca
     3781 cgacctcttt gaaacccctc tctctggcca tgaaaagcgg aatgttcgaa tatacgccgc
     3841 catctatgaa gagctcatta ccgatctcct ctctcttgaa agccggaaag ttagcgctgg
     3901 caagaacata atccacaaga aggcctttgg gacagttttc tttgtagatt acaaatggcc
     3961 tgacatcgct caacgagtaa gcgaccacac cgaagtccat tcggcttttt cgtattttct
     4021 cctcgcttat tagtttttgg agcttttccc gcaaaggcga gacgtctatc ccccccttag
     4081 ccgtaagtaa tctcaacgct gaggccaact ctaaagtatt aagggagaaa aactttccct
     4141 ggagaagatc gtagagctcc ggagaggctt tcatcagctg cgtatagtcc ataccgtacc
     4201 aaaaatccct ggttgcttca tagccttcgc tgattatacc cgcggcgttt atcgctccca
     4261 ctgatgcacc gaagatcccc tcaatctcta tatccagatc caggagagct ccgtaaacac
     4321 cgacctgata ggctccacgt cccccgcctc cggagaaaac aaccgctaca gtcaaaaggc
     4381 ttgaaaggaa cagggtacag aatatcgcga gcaataaaaa ccttttcaac cggatacacc
     4441 tgccaatgtc tgtttgatcg aatccgttga tgatattata atacatttaa gaaaatgacc
     4501 gacagttgat cgggagaatg ttaatctttc catcaccagt ggaagaggct tatctcttat
     4561 gattgataac gtcagttaag ctaacttgtg gagtggtgca gttgataagg tatttcttga
     4621 agcaaaactg gtacagatac ctaatcggta tccttttttt gatctccgtc gatacactca
     4681 tgcttataac acccagaatc atgggaagta tagtcgatga actaaggagc ggcgtccagg
     4741 atatggaata cattgggctc cttatactgg cagttatagg tgtcgccttc gggttgttca
     4801 taacacgtct cttctggcgt gtattcataa tgggatcggc caggagattc gaatactacg
     4861 ccaggaagac gctttttcaa aaactgctgg acatgccccc aactttctac gacaagacga
     4921 gggtgggaga tctgatggcc agattcacca acgacgtcaa cgccgtgagg atggcgatgg
     4981 ggccggcgat agtcatgacg atagattcga tcttcctcat atccgtcacg ataatagcaa
     5041 tgggctcctt caccagctgg gagctcacat ggattgcgat catccccctg ccccttctag
     5101 cggtggtatc gacatttttc ggcagaatca ttcacaagag gttcaaaaaa gtccaggctg
     5161 ccttttcaaa cctgaccgac acggtcgagg aatctgtctc cggcgccagg ttgataaaga
     5221 gttacggtat agaaggattg agaaacgata cattgagcga gaaatccgac agctacgttc
     5281 aggagaatat aaagttgata aaagtgtggg gaatgttctt tcccctaata cagatgctat
     5341 ccatgctcgg ttcggtgctg gcaacgcttt ttggagggca gatggtggtt cttggccagc
     5401 tcacactcgg agaatatgtc accttcacat catatctggg aatgcttatc tggccgatga
     5461 cggccttcgg gtgggtaatt aacatggttc agcgaggacg tgcttcttat aaaaggttgc
     5521 tggaggtact tgaagagaag aacaacatag ttgtcgaaga tccagtatcg ctcgaggatt
     5581 tcaacggcca tatttcactg aaaagactca cgttcacata tcctaccgga aatcgtccag
     5641 ttctccatga tatagatttc gaagtgatcc ccggttcaaa ggtcgcgata gttggaacca
     5701 ccggatctgg caaatctaca ctggcgaaat tgatcgcccg tctctacccg gtggaaaagg
     5761 gaatgctttt catcgatgga tacgatataa accgtcttcc acctgaggtc ataagagaga
     5821 ggattgccta cgttccgcag gagacctttc tcttttcgga aacgataagg aacaacataa
     5881 ggttcggtaa tatgaaactc accgatagcg aggtggaaaa gtacgcggcc atagcatcga
     5941 ttcatgagga tatactgggc tttcccaaag gttacgacac acttgttgga gaaaggggag
     6001 tgacgctttc cggtggccag aagcagagag tggccatcgc ccgagctctt atcaaacaga
     6061 cgcccattat tgttctggac gattgcctct cagccgtcga tgcggagacc gagaagaaga
     6121 tactcgactc cctgaaagac tcacagggga aacgttcgat agtggttgtc tcccacaggc
     6181 tcaaggccgt agtagatgcc gatacgattt atgtcatgca cgaaggaaag atcgttgaga
     6241 gaggtacgca tcacgagctg gtcgagaatg gaggcctgta ccagcgtatg tacgaacgtc
     6301 agatgataga ggaaaagctc aaggaggatt agcgatggcc caaaatgtat atagcgaagc
     6361 ggaagaaaaa ctgaaagtcg aagattggaa agtgatcttc aggctttgga aatacaccaa
     6421 gccttacctc ccgatactca ttctggcact cgttctcatc ggtgcctcca cgggattgga
     6481 tcttctccca cctctcctgt cgaagatggt gatagacgat tacatgaaca atgaatacac
     6541 gctcgttgtg attcacgagg gagattcggt caggttcatc gatactgaag agggaacctt
     6601 ctcgctgaaa cagtcagaaa gcggtgatta cgtcgtcact gacggagttc tacaataccc
     6661 tatcactcgg gagcaactac gtgaactgag gactgaagat ctgaac
//
LOCUS       DAOOLY010000125         6683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13632_length_6683_cov_4.320911, whole genome shotgun sequence.
ACCESSION   DAOOLY010000125 DAOOLY010000000
VERSION     DAOOLY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13632_length_6683_cov_4.320911"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1127)
                     /locus_tag="PLD15_07250"
     CDS             complement(186..1127)
                     /locus_tag="PLD15_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63738.1"
                     /translation="MPAEITAVDVVRILGTLSDEGGDRSSRNRFIHGYLTKYDRPEPV
                     LADIENLLASYHGFGGDIESLERAYGDLLNRLAEVAGFSVDYIKYSGREKITGIWKVN
                     ESFEIRVSAIIGKSFEEAKGIARNALLILPQELELGRPFVTVKRLGELFTMVSQIELP
                     VSSVASILMTDDDYDKRIASFMELIFLAFRKELSRKVQPSSLKREWESNELNDFIKSL
                     KLPTVGMLFALSESPCNGEELVERINHFLEKAGGERLKSPMALGAIMGALSKHYSGIK
                     EELVLREGKSYRVAEKYRESISEIKTRFWRRFQEDNR"
     gene            complement(1266..2108)
                     /locus_tag="PLD15_07255"
     CDS             complement(1266..2108)
                     /locus_tag="PLD15_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPB63739.1"
                     /translation="MRISVVGNINIDLNAVIGDSVNSEENRIKKLGLSSGGSAANTAI
                     ILSRLGREVFLQGAVGDDLFGDYLIERLSRERVETGYIKVLSGGTGLCFAAIGEQGQR
                     HLYTYRGANERYETVAGGFDFYHFAGIATGQLSVMLNSLNIERFSYNPGGIVTFERPR
                     EVSKLAERAEVLFLNETEWAFLSPFLNRTNTVVVTMGAAGSRIESGPSVEALPVEVVD
                     TTGAGDAFNAGFLHAYLSGRQGSDCLKVGNLLGAIVAMHVGATPEFEYGYILEKSELY
                     KVKL"
     gene            2205..3443
                     /locus_tag="PLD15_07260"
     CDS             2205..3443
                     /locus_tag="PLD15_07260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HPB63740.1"
                     /translation="MVAFPEGFLWGVSTAGHQIEGGNRHSDWYEWEKKGKVKGGETSG
                     VACGSWDHLERDIKALEELGVKAYRYSIEWARIEPESGKFDEDALKRYAGFTEELVRK
                     GIHPVLTVNHFVLPLWFARMGGWEKRENLIHFEKFVRKVVQSIGQYVHYWVTINEPNV
                     YASMSYLLGEWPPQKKDMELARKVLTNLIFAHTVAYDAIKSAVPTAMVSIALNMMPFL
                     PFRNWHPADFIAARFLGKLYNYSFVDSIKLGRMARPLGNEEAAPEIKGKIDFLGINYY
                     SRTFVRYSRPFPQLLNGQEFERTDMGYEFFPQGLEGVVFKAYKRYRLPIMITENGIAD
                     STDEKRWPYIDGALQALGKSMNRGAKVFGYLYWSLMDNFEWKEGYSMKFGLYETVRDT
                     LELKARPSAEKFRRFISENR"
     gene            3674..5668
                     /locus_tag="PLD15_07265"
     CDS             3674..5668
                     /locus_tag="PLD15_07265"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPB63741.1"
                     /translation="MELISALIAVTVSLFSVILGIYLFRWVGRHPVGSERIREIGEAI
                     REGADAFLKKEYVILAFFSIAICILLAIFLSISSAIAFLVGAISSGVAGLLGMKVALK
                     ANVRTATAADHEGLEKAFLIAFRGGSVMGLLVVGLALAGVSVIYGIALKAEILLSFSF
                     GASILSLFAKAGGGIYTKTADISADLVGKVEIGIPEDDPRNPAVIADNVGDNVGDVAG
                     MGADLFDSYVASIVAAMVLGETLGVKMVVLPLILASVGVLSSLVGIFSVRVRGEKNPG
                     RALDLGTYITSAIFALATLVISLALSYDLKFWGAAMAGLVAGTVIGFTTDYFTSIDRH
                     FVKRTAQASQSGAAITILTGYSYGLMSLLPSLVGIGVASGVAFALCGVYGIAVSAVSM
                     LAIVGMIISSDAYGPIVDNAKGISEQSGLSEEVISLTDKLDAAGNTSKAITKGFAIGA
                     AGLTVIALLATYQERVAAITGQSITFDLMNPLVMLGLLTGLCVPALFSAMIMLGVSKN
                     AFRMIAEIRRQFKEIKGLSEGTARPDYARCVEIATTGALKELMLPSLISIGITLLIAF
                     IGGIYALGGFLGGAILSGLFNALLMANSGGMWDNSKKYIEDGAFGGKGSDAHKAAVIG
                     DTVGDPFKDTAGPSLNTLITVLSLVASLISPLVILYAVFK"
     gene            complement(5720..6289)
                     /gene="yihA"
                     /locus_tag="PLD15_07270"
     CDS             complement(5720..6289)
                     /gene="yihA"
                     /locus_tag="PLD15_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPB63742.1"
                     /translation="MIKSVELCRTVFKSGEYPPPLEREIAFAGRSNVGKSSLLNSLFG
                     RKLAHTSSTPGKTRSINFYTVNGSVYFVDLPGYGYAKASIKEKEKWERLIIDYFDSRK
                     SLTLLAVLMDSRHPFQAVDMQMLEWVIYYSLPYLVVLTKADKLSRSQQSITLKSVKDG
                     LSESGALPPVFMVSAEKRQGTEELINTLL"
     gene            complement(6290..>6683)
                     /locus_tag="PLD15_07275"
     CDS             complement(6290..>6683)
                     /locus_tag="PLD15_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPB63743.1"
                     /translation="RKLCDGEKYAEIFEKRDFHIHVPEGAVPKDGPSAGITLTTAFIS
                     AVSGRKVRNDVAMTGEITLRGKVLPIGGLKEKLMAAYRSQMKTVIVPLANKRDLEKVP
                     EEIKSRLEFIFVEDIMEVLQIALLDKGD"
BASE COUNT         1550 a         1725 c         1686 g         1722 t
ORIGIN      
        1 gtcataatca acattatgta tcattttatt tattgtacca catgaggtta tttccacaac
       61 cttgcaaaag cctttaaaat agccatttac agtactagac cttttaaaat gaccgataac
      121 gagtttttcc gtccccgcat gcctttttac ctatagatcg ccctaaaaat gcaacaggga
      181 gcgttttatc gattatcctc ctgaaaacgt ctccagaacc gtgttttgat ttcggagata
      241 ctttctctgt atttttcggc gactctgtaa ctctttccct ccctcagtac gagctcttcc
      301 ttgatacccg agtagtgttt cgacagtgca cccattatcg caccgagtgc catgggactt
      361 ttcagtcttt ctccacccgc tttctcgaga aagtggttta ttctctccac aagttcttct
      421 ccattgcagg gtgactccga aagcgcgaaa agcattccga cggttggcag tttaagactt
      481 ttaatgaaat cgttcaactc gttgctctcc cactctcttt tgagacttga aggctgtacc
      541 tttctggaga gctcctttct gaaagccagg aagattagtt ccatgaagga agcgatcctc
      601 ttgtcgtaat cgtcgtcggt catgaggatc gaggccaccg aactgacggg gagctctatc
      661 tgcgagacca ttgtgaacag ctctcccagt cgtttgaccg taacgaaggg tcttcccagt
      721 tccagttcct gcggaaggat cagcagcgcg tttctggcta tccctttggc ctcctcgaag
      781 ctcttcccga ttatagccga gacccttatt tcgaagcttt cattgacctt ccagataccc
      841 gttatctttt cccttccgga gtacttgatg tagtccacag aaaagccggc cacttcggct
      901 aatctgttaa gaagatcgcc gtaagccctc tccaggctct cgatatctcc tccaaaaccg
      961 tgatagctgg cgagaaggtt ttctatatcg gcaagtacag gctcgggccg gtcgtattta
     1021 gtcaggtaac catgaatgaa cctgttcctg ctcgaacgat cgccaccttc gtccgaaagc
     1081 gttccgagaa tccttacaac atctacggcc gttatttcag ctggcatata cttcaccccc
     1141 taacttatac taccacgaat atatacgatt ctgcagacaa aatataatcc atatattcaa
     1201 gaacaatagt gattatataa gtataattta tatattcaga ttcacattaa atatgaaact
     1261 atatatcaca acttcacctt gtacaattcc gatttttcta gtatataccc gtattcaaat
     1321 tcaggtgtag caccaacatg catagctaca attgcgccta gaagatttcc gaccttcaga
     1381 caatcactgc cttgtcttcc ggagagatac gcgtggagga aaccggcgtt gaaggcgtct
     1441 ccggcgcctg tggtatccac gacctctacg ggtaaggcct cgaccgaggg accgctctcg
     1501 atcctcgaac cggccgctcc cattgtcact acaacagtgt ttgttctgtt caggaagggt
     1561 gaaagaaagg cccactccgt ctcatttaag aaaagaactt cggccctttc ggcgagcttt
     1621 gagacctcgc gcggtctttc gaaggtcaca atgccgccgg ggttgtagga aaacctttct
     1681 atgttcagcg agttcagcat caccgatagc tgaccggtgg caatgcctgc gaaatggtag
     1741 aaatcgaatc ctccggctac agtttcgtac ctctcgttcg cgccacggta agtgtaaaga
     1801 tgtcgttgtc cttgctcgcc gatagccgca aaacaaaggc ctgttcctcc gctaagcact
     1861 ttgatgtacc cggtctcgac tctctcgcgt gacaaccttt cgatgaggta atctccgaag
     1921 agatcgtctc ccaccgcccc ctgcagaaag acttcccttc caaggcgcga gagaatgatc
     1981 gccgtgttgg ccgcacttcc tccagaggag agacccagtt tttttatcct gttttcctcg
     2041 ctgttaacgc tgtctcctat tactgcgttc aagtctatgt ttatgtttcc aacgactgaa
     2101 atcctcaatt ttctctccct ttcccgatta ggtttacgtt ctttctctct ttataattat
     2161 aattggaagt gataggaaca gagctttcga ggggggtgaa aactatggtt gcgtttcctg
     2221 aaggttttct atggggtgtt tcaacggccg gtcatcagat agaaggtgga aaccgtcact
     2281 cggactggta cgagtgggaa aagaagggga aggtaaaagg cggggagact tccggggtgg
     2341 cctgcggaag ctgggatcac ctcgagagag acatcaaggc tcttgaggag ctcggggtga
     2401 aggcttaccg ttactccata gagtgggcca gaatcgaacc ggaaagcggt aaattcgacg
     2461 aggacgctct taaaaggtat gccggcttca ccgaagaact cgtaaggaag ggtatccacc
     2521 ctgtgttgac agtgaaccac ttcgtcctgc cgctgtggtt cgccaggatg ggtggttggg
     2581 agaagcgtga aaatctgatc catttcgaga agttcgtcag aaaggtggtc cagtccatag
     2641 gccaatacgt tcactactgg gttaccatca acgagcctaa cgtctatgcc tccatgtcgt
     2701 atttgcttgg agaatggcca ccgcagaaaa aagatatgga actggctcgt aaggttttga
     2761 cgaatctgat tttcgcgcat acggtggctt acgacgcaat aaagtcggcg gttccaacgg
     2821 ccatggtgag catcgccctg aacatgatgc ccttccttcc ctttagaaac tggcatccgg
     2881 cggactttat cgccgccaga tttctgggaa aactctacaa ctattctttt gtcgactcta
     2941 taaaactcgg caggatggcc aggccactgg ggaacgagga ggccgcaccg gagataaagg
     3001 gaaagatcga ttttctcggt ataaattatt acagccgcac cttcgtcagg tattccagac
     3061 cctttccgca attgctcaac ggtcaggaat ttgagaggac ggatatgggt tacgagttct
     3121 tcccgcaggg tctggaagga gtggtcttca aagcttacaa aagataccgt ttgcctataa
     3181 tgattacaga aaacgggata gccgactcca ccgatgaaaa gcgctggcct tacatcgatg
     3241 gcgccctaca ggcactcggc aaatctatga acaggggagc gaaggtcttc ggttatcttt
     3301 actggtcttt gatggacaac ttcgaatgga aggaaggtta ttcgatgaag ttcggtctct
     3361 acgagaccgt tcgcgataca ctggaactca aggcgagacc tagcgccgaa aagttcagac
     3421 gtttcatatc tgaaaaccgg tgatcgaaag ggtcgtggag gtgattggat ggacgagcta
     3481 acatatatgc ttgccccgtg cggcatcgac tgcgatgggg ctcgatttac ctaatgactc
     3541 ctttcgaata ttcccaccac cttgcagaca ttcagattta acctgtcgat cgataccatt
     3601 ccgccgatat gtggtgctag aatcatgttg acttctcatc gtgtggttcg tccataaggg
     3661 ggcgtaaatg aatatggaac tgatttctgc actgatagcc gtgacggttt ccctgttttc
     3721 ggtaatcctc ggtatctatc tctttcgctg ggtcggcagg cacccagtcg gtagcgagcg
     3781 cataagggaa ataggcgagg cgatccgcga gggagccgac gcctttctaa agaaggaata
     3841 tgtgatactg gccttcttct caattgcgat ctgcatcctt ctggcgatat ttttgagtat
     3901 ttcttcagcg atcgccttct tagtcggagc catctcttct ggcgttgcgg gtctactggg
     3961 aatgaaagtg gcgctcaagg ccaacgttcg tacagcaacg gctgcagacc acgagggcct
     4021 cgaaaaggct tttctgatag cttttcgggg cggttcggtg atgggtctgc ttgtggtggg
     4081 actggcactt gccggtgtga gtgtgattta cggtatagct ctgaaggcgg aaatacttct
     4141 gagcttcagc ttcggggcga gcatcctttc gctttttgca aaggccggtg gaggtattta
     4201 caccaagacg gccgatataa gcgccgacct cgtcggcaag gtcgaaatag gaattcccga
     4261 agacgatccc agaaatccgg ccgtcatcgc cgacaacgtc ggtgacaacg tgggagacgt
     4321 ggccggtatg ggagccgatc tcttcgattc ttacgtggcc agtatcgttg ccgcgatggt
     4381 cctcggagag actctgggag taaagatggt ggtcctgccg cttatcctgg catcggtcgg
     4441 ggtcctctcc tcgctggtcg gaatcttcag cgtccgtgtc agaggggaga agaatcccgg
     4501 acgcgccctt gaccttggaa cctacatcac ctcggcgatt ttcgctctgg caacgctggt
     4561 gatctccctt gcgctctctt acgatctaaa attctggggc gccgcgatgg ccggcctcgt
     4621 cgccggaacg gtgataggct tcaccacaga ttatttcact tccatcgaca gacacttcgt
     4681 gaagagaacg gcccaggcct cgcaatcggg tgcggctatc actattttga cgggctactc
     4741 ttacggattg atgagccttc tcccatcgct ggtgggaatc ggtgtagcct ctggcgtggc
     4801 ctttgccctg tgcggagttt atggaatagc cgtaagcgcc gtctccatgc tcgcaattgt
     4861 tggaatgata atctccagtg acgcttacgg ccctatcgtg gacaacgcca aaggtatctc
     4921 cgaacagtct gggcttagcg aggaagtcat atcgctaacc gacaagctcg atgctgccgg
     4981 aaatacttcg aaagctatca ccaaaggttt tgcgataggg gcggccggac tcacggtcat
     5041 agcgctgctg gcgacttacc aggaacgcgt ggcagccatc accggccaga gcataacctt
     5101 cgatctcatg aatccgctgg tgatgctcgg tcttctgaca ggcctttgcg ttcccgcact
     5161 cttttcagct atgataatgc tgggcgttag caaaaacgcc ttcaggatga tagccgagat
     5221 caggaggcaa ttcaaagaga tcaagggtct cagcgaagga acggccagac ccgattacgc
     5281 gcggtgtgta gagatagcta cgacgggcgc tctaaaggaa ttgatgcttc ccagtctgat
     5341 ctctatagga atcaccctct tgatagcctt cataggcggg atatatgctc tgggaggctt
     5401 tttgggcgga gccatcctct cgggactgtt caacgcattg ctgatggcca acagcggagg
     5461 catgtgggac aactcgaaga aatacataga ggacggcgct ttcggcggaa agggctccga
     5521 cgcgcacaag gcagccgtaa tcggcgacac tgtgggagac cccttcaaag acaccgccgg
     5581 accttcgctg aacactctca taacggttct ctccctggta gcctctctga taagcccgct
     5641 ggttattctt tacgccgttt tcaagtgatt atctactgaa aaaggcgggg aaaatccccg
     5701 ccttttctat tgccgcagat cagaggagcg tgttaatcag ctcttcagtt ccctgtctct
     5761 tttcggccga gaccatgaac acggggggca gagccccact ctccgacaga ccgtctttga
     5821 cggacttgag agttatggat tgctggcttc tcgaaagctt gtcggccttc gtcagaacga
     5881 ccaggtacgg cagggagtag tagataaccc attccagcat ctgcatgtca acggcctgaa
     5941 aggggtggcg gctgtccata agaactgcca ggagcgttag agactttctc gaatcgaagt
     6001 aatcgattat gagtctctcc cacttctcct tctcctttat ggaggccttt gcgtaaccgt
     6061 aaccgggcag atccacgaaa tagaccgacc cgttgacggt gtagaagttt attgaacggg
     6121 tcttgcccgg agtcgaactc gtgtgggcca gcttcctccc gaaaagggag ttgagaagcg
     6181 aagacttgcc gacgttagac cggccggcaa aggctatctc cctttccagc ggaggcgggt
     6241 actctcctga cttgaagacc gttctgcaca gctcaacgct ctttatcact caatcacctt
     6301 tgtccagcag agcgatctgg aggacttcca tgatatcctc cacgaaaatg aactccaacc
     6361 gggattttat ctcttcggga accttttcca gatcccgttt gttagccagg ggtacgatca
     6421 ccgtcttcat ctgggaacga taagcggcca tcagcttctc tttcaatccg cctatcggca
     6481 gaacctttcc ccgtagggtt atctcgcccg tcattgcgac atcgtttctt acctttctgc
     6541 cggaaacggc cgaaatgaag gctgtagtca gagttattcc agcactgggt ccatctttgg
     6601 gaacggctcc ctccgggaca tggatgtgga aatccctctt ctcgaagatc tcggcgtact
     6661 tttcaccatc gcagagcttt ctg
//
LOCUS       DAOOLY010000126         6651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13719_length_6651_cov_5.727865, whole genome shotgun sequence.
ACCESSION   DAOOLY010000126 DAOOLY010000000
VERSION     DAOOLY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13719_length_6651_cov_5.727865"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1803)
                     /locus_tag="PLD15_07280"
     CDS             complement(94..1803)
                     /locus_tag="PLD15_07280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPB63744.1"
                     /translation="MTDEKIVKQPRRYLPEDLDLSVWKNVEAELKRLEEYPVDSVDSL
                     EEFLYRWSELYMVIQEQLAWRYIEMTRFADSEEKRKNYSNFYAGVFSRAEPYKMRLLK
                     KYYDSPYRAELDTLRYENFDRIVANTIEMFREENLPLEIKEKELSSAYGATIGSLTAE
                     YDGKEYTLSQLSRFQQEPDRKVREETWRLSMEKLSTVHEKLEELFDQLKEIRIPQARN
                     AGFENYRDFMHARKNRFVYSVEDIFRFHDSVEAVVVPFVKKLNEGRRRELGVESLRPW
                     DTQVEPSGRVLKPFDGMEDFIGKAVAVLSRVDRQFGLNLQKMKNTELLDLENRKGKAP
                     GGYNYPLDETGAPFIFMNATGTPGNVRTILHESGHAMHSFSTVDERIILYRHTTHEAA
                     ELASMSMELLTMDHWDEYYPDGEELKLAKKEELTGTISFLPWCMTVDAFQQWIYSNPD
                     HTPDERDEKFAGIFARFEGGIDWSGLEGERRTRWLLQPHIFTNPFYYIEYGIAQLGAL
                     AIYRNYRLDREKAVADYKRFLSLGYSRPLDELYEAAGIKFDFSESYISELVDFVEGEI
                     ERL"
     gene            2331..3053
                     /locus_tag="PLD15_07285"
     CDS             2331..3053
                     /locus_tag="PLD15_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63745.1"
                     /translation="MNGLERRLDRMFSRGSLIVAAADHGQFAGVPQGLEDIGRYVSEI
                     ETLDIDGILLNPGVVSRIASFDANKLLIIRVTHAGSALSGDIGNTKYFLDPEQALRLG
                     ADAVIVMGIVGQERDSDALHALSKAIWDYQRYGIPVIAEMLPYRKEDYSSPEVIGNIS
                     RIGAELGANVIKTMMTANYGEVVRSCPVPVIVAGGEKNRDFFASLEDAAKAGAKGAAI
                     GRNLFQEKERTGFLKKVREVFT"
     gene            3064..4026
                     /locus_tag="PLD15_07290"
     CDS             3064..4026
                     /locus_tag="PLD15_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HPB63746.1"
                     /translation="MKMDEVSLSYRAAEMYYINEMNQEEIAIELGLSRSKVSRLLTSA
                     RRLGMVKIELVGPESFDLSSLEREIVEKYAIREAHVVASIKGTDKEIKQRISLSFQKN
                     LTKLLEGKRYVGLGWGTTVYEAVMSLPENLARFPEMKIIPLLGGLGQSEKTYQINNIV
                     EKMAGSLGAIPIFLAAPAIFNNPEQLKIMKQVQSVQTAVNEWSNLEAVVFGLGAPAGM
                     SAVLDSNLPGDMILELVRKHAVGDIVSRFMGKGGEIVCQNIEEVLLGIPFGDLLKVPE
                     RICLSGGEHKVEGIRAALSRGYITTLFTDIRTAQMLVTQQEVHG"
     gene            4023..5006
                     /locus_tag="PLD15_07295"
     CDS             4023..5006
                     /locus_tag="PLD15_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB63747.1"
                     /translation="MKAVMYEGIGKISIRDVEKPKIGEDGILVKILYSFICSTDIKTY
                     KQGHPMIKPPTILGHECSGRIVETGRNVSEFEVGDYVAVAPFVNCGECEQCINGYPEG
                     CRTRDFPSNGAITEFLSIDAGYARKGVAKVSKEKVKEAALAEPMACALTSCRHMELQP
                     GNSALVVGAGIMGMLNAITLRDVYGQTVVITDKRKERLELAEELGFAASSEDTGKYNS
                     IVLTAPVPELIDEYLPRVKLFGNMVLFGGYSKGTKALFDPNIIHYNGVKLTGTTGFAS
                     RDFMAAIALINSGKLDLRSFTKRIYEFSDFEEAFKDAVEGRSIKVGIKVGE"
     gene            4999..5793
                     /locus_tag="PLD15_07300"
     CDS             4999..5793
                     /locus_tag="PLD15_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63748.1"
                     /translation="MSELFGKANRLSRIFNRSGKTIMLALDHGMALGPMQGLERPAEM
                     LGKLTPFTDSIMLNKGILQNCYIPNGHVGIVLRVSGAATIAGEDITNESITTTVTEAL
                     RLSADAVATSIYVGTPNEYSTIRELARLCDDCETFGLPVLAVTAIGKDRERKTDPRYL
                     SLAVRVAVEMGADIVKTYYCDEKFEKVIEAAAGIPVVIAGGPKMDSVLDVLKIAENAT
                     RAGARGVDMGRNVWQHERPVEMLLALKDIVNDGMPAEEAYSKRFGR"
     gene            5796..>6651
                     /locus_tag="PLD15_07305"
     CDS             5796..>6651
                     /locus_tag="PLD15_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB63749.1"
                     /translation="MKVVEMISVRKEFPGVVALDNIDFALEEGEIHGLVGENGAGKST
                     LINILAGVFQQTSGELRLFGKPVKLSSPSEALHRGVGVVYQDTVLDPFFTAEENIWLG
                     REPSRLSVLKGREIRSNTKRLCEKYGIEVPLRVPVGTLSVAKQKMVEILRALSMNSKV
                     LVLDEPTASITQSDTDNLFEILKNLKKSGISIIFVSHHLDEVFNLCDRISVLRNGRYE
                     GTSVSSELSTRDLIKLMTNRDLVDQYPKQASEIGEGILKVSKLNVARLGLKDISFEVK
                     RGEIVGFFA"
BASE COUNT         1608 a         1705 c         1798 g         1540 t
ORIGIN      
        1 cctcctaaac tattctattc tcaacggctt tccgtgagtc ctgttttcga gactctgctc
       61 cccacaaata aaaattacga ccgtatcgtt tttctagagc ctctctatct ctccctcgac
      121 aaaatcgacc agctcggata tatacgactc cgaaaagtcg aacttgatcc ccgccgcctc
      181 gtacagttcg tcgagaggtc tggagtatcc cagggagaga aaccgcttgt aatcggctac
      241 ggccttttcc ctgtcgagtc tgtagtttct gtatatggca agggctccga gctgggcgat
      301 accgtactca atgtaataga aggggttggt gaagatatgg ggctgaagca gccagcgggt
      361 tcgccgctcc ccctctagac cggaccagtc gatcccgcct tcgaacctgg cgaagatccc
      421 ggcgaacttc tcgtccctct cgtcgggcgt gtggtcggga ttggagtaga tccactgctg
      481 gaaggcatcg accgtcatac accagggcag gaagcttatc gttccggtca gctcctcttt
      541 tttcgccagt ttcagctctt cgccgtccgg gtagtattcg tcccagtgat ccatggtgag
      601 cagttccatc gacatcgaag ccagctcggc cgcttcgtgc gtcgtgtgtc tgtacagtat
      661 tattctctcg tccacggtcg agaaggagtg catggcgtgg cccgattcgt ggagaatagt
      721 tctgacgttg cccggcgttc cggtggcgtt catgaatatg aagggcgccc ccgtctcgtc
      781 cagcgggtag ttatagcccc cgggagcctt cccctttcta ttttccagat ccagcagttc
      841 tgtgtttttc atcttctgca ggttgagacc gaactgtctg tctactctgg agagaacggc
      901 gacggccttt cctatgaagt cctccatgcc gtcgaagggc ttcaataccc ttcccgaggg
      961 ttctacctgc gtatcccagg gccttagcga ctccactccg agctctcttc tccggccttc
     1021 gttcagtttt tttacgaagg gaacgaccac ggcttcgact gaatcgtgga acctgaagat
     1081 atcctcgacc gaatagacga acctgttctt cctggcgtgc atgaaatctc tgtagttttc
     1141 gaaaccggca tttctggcct gaggtatcct tatctccttt agctgatcga aaagctcctc
     1201 cagcttttcg tggacagtgg aaagcttctc catcgagagt ctccaggtct cctctctcac
     1261 ttttctgtcg ggctcctgct ggaatctgga gagttgagag agcgtgtact ccttaccgtc
     1321 gtattctgcc gtcaaagagc ctatcgtagc gccgtaggcg gaggataact ccttttcctt
     1381 tatctcgagc ggaagatttt cctctctgaa catttcgatg gtgttggcca ctatcctgtc
     1441 gaagttctcg tacctgagag tgtcgagctc ggccctgtag ggactgtcgt agtacttctt
     1501 cagcagcctc attttgtagg gttcggctct ggagaagacc ccggcgtaga agttcgaata
     1561 gttcttcctc ttttcttcac tgtcggcgaa tctcgtcatc tctatgtatc tccaggccag
     1621 ttgctcctgg atcaccatat agagttcgct ccatctgtag agaaactctt ccagagaatc
     1681 cacgctgtcc acaggatact cttcaagcct cttcagctcg gcctcgacgt tcttccacac
     1741 cgaaagatca aggtcctccg gtaaatacct gcgcggctgc ttcacgatct tctcatctgt
     1801 catctcttaa cctcccgtac tggatttatt agattagcga cgtaaacgat tttgccacaa
     1861 atgctttcct tccggcaagg tttttccata cctcagcacc gaagaacaat cttgacaact
     1921 tagaagagac gtccttaggg aaccgtaacg ctgaacctga ggctagaggt aagaggggag
     1981 aggtgtgaag agcgtgaagg tcttgacttt ctaacctcta acctgtcacc tctaacctct
     2041 gtttaagttt gcgagagagc gacgagaaga acgagatccc gtactgcgaa aaaacagacc
     2101 tcaggagccg gtcagtcccc attttcgctc tctcttttct ctcactatct ctctttctct
     2161 cctcttctcg cgttccttaa ccaactaaga acctggacgc gcagcgtcgg aacgaataat
     2221 cgctttcccc acgacgcggc cgccttgact tggcgctttc aaaagtgata ttataggtct
     2281 gaagtgaata attgtgatgc acacttgtgc aagaggaatg gaggaacttt atgaacggtc
     2341 tggaaagaag attggacaga atgttttccc gtggttctct gatcgttgct gcagccgatc
     2401 acggccagtt cgccggtgtt ccgcagggtc tggaggatat cggacgctat gtgagcgaga
     2461 tagaaactct cgatatcgat ggaatactgc tgaaccccgg agtggtttcg cgaatcgctt
     2521 ctttcgacgc gaataaactt ctgattataa gggtaacgca cgccggttcg gctttatcgg
     2581 gcgatatcgg caacacgaaa tactttctcg atcccgaaca ggcactgagg cttggagccg
     2641 atgcggtaat agttatggga atcgtcggtc aggagagaga ttccgatgcc cttcacgctc
     2701 tttcgaaagc catctgggac taccagaggt acgggatacc tgtaattgcc gagatgcttc
     2761 cttacaggaa ggaggactac tcctcccccg aggttatagg aaatatatct cgaataggag
     2821 ccgaacttgg agcgaacgtt ataaagacca tgatgaccgc aaattacggc gaggtcgttc
     2881 gatcgtgccc cgttcccgtg atcgtcgcag gaggcgagaa gaaccgcgat ttcttcgctt
     2941 cccttgaaga cgcggcaaag gccggcgcta aaggcgcggc gataggtcga aacctcttcc
     3001 aggaaaagga aaggaccggt ttcttgaaga aagtcagaga ggtattcacc tagaggtaag
     3061 attatgaaga tggatgaagt cagcctctct tacagggccg cagagatgta ttacatcaac
     3121 gagatgaacc aggaggagat agccatcgaa ctgggtcttt cgaggtcgaa agtctccagg
     3181 ttgctcacca gcgcgcgcag actgggaatg gtgaagatcg agctcgtcgg gccggaatct
     3241 ttcgatctct cctctctcga gagagaaata gtcgagaaat acgccataag ggaggcccat
     3301 gtcgtagcct ctatcaaggg caccgataag gagataaagc agaggatctc tctctccttc
     3361 cagaagaacc taaccaagct tctagaaggc aaacgttatg tcgggctggg ctgggggacg
     3421 accgtttatg aagccgtaat gagtctcccg gaaaacctcg ccaggtttcc cgagatgaag
     3481 ataataccgc tcctcggggg tctgggacag tcggagaaga cttatcagat caacaacatt
     3541 gtcgaaaaaa tggccggttc tctgggcgcg atccctattt ttctggccgc tcccgcgatc
     3601 ttcaacaacc ccgagcagtt gaaaatcatg aagcaggtac agtcggttca gacggccgtc
     3661 aacgagtggt cgaacctcga agcagtcgta tttggtctgg gagccccggc cggaatgtcg
     3721 gccgttctcg actcaaatct tcccggcgat atgatactcg agctggtcag aaagcacgcc
     3781 gttggcgata tagtctctcg cttcatgggc aaaggcggcg aaatagtctg ccagaacatc
     3841 gaagaggtcc ttctgggaat ccccttcggc gatctgctga aagttcccga gaggatctgc
     3901 ctgagcggcg gggagcacaa agtcgaagga atacgggcag ccctttcaag aggctacata
     3961 accactctct ttacggatat aaggacggcc cagatgctag ttacacagca ggaggtgcat
     4021 ggatgaaggc agtgatgtac gaaggaatcg gaaagatctc aattagagat gtcgagaagc
     4081 caaaaatcgg agaagatgga attcttgtca agatactcta ctcattcatt tgttccacag
     4141 atataaagac ctacaagcag ggacacccga tgatcaagcc tccgaccata ctgggccacg
     4201 aatgttcggg aaggatagtc gaaaccggtc ggaacgtttc ggagttcgaa gtcggcgatt
     4261 acgtcgccgt agcccccttc gtgaactgcg gcgagtgcga gcagtgtatc aacggttacc
     4321 ccgagggatg cagaaccaga gactttccca gcaacggagc cataaccgag ttcctttcga
     4381 tcgacgccgg ttacgccagg aagggcgttg caaaggtttc gaaagaaaag gtcaaagagg
     4441 ctgccctcgc ggaacctatg gcctgcgcgt tgacctcttg cagacatatg gagctgcagc
     4501 cgggcaactc ggcgctggtc gtcggggccg ggataatggg tatgctgaac gctataacct
     4561 tgcgggatgt ttacggccag acggtggtga ttaccgacaa aaggaaagag aggctggaac
     4621 tggccgaaga actgggcttt gccgcctcca gcgaagacac gggcaaatac aactcgatcg
     4681 tgctaacggc gcccgttccc gaattgatcg atgaatacct gccccgggtg aagttgttcg
     4741 gaaacatggt gctcttcggc ggctattcga aagggacgaa agctctcttc gatcccaata
     4801 taatccacta caacggtgtg aaattgaccg gtaccacggg ctttgcctct agggatttca
     4861 tggccgccat cgcgcttata aacagcggca agctcgactt gagatctttc acaaaaagaa
     4921 tctacgaatt ctccgatttc gaggaggcct tcaaagacgc cgtagaaggc agatctatca
     4981 aagtcggaat aaaggttggt gagtgagttg ttcggcaagg caaacagact ttcaaggata
     5041 ttcaacagat ccggaaaaac tataatgctg gctctcgatc acgggatggc tctaggaccc
     5101 atgcagggtc tggaaagacc ggccgagatg ctaggcaaac tcacaccgtt caccgattcc
     5161 ataatgctca acaaagggat actgcagaac tgctacatac cgaacgggca cgtcgggatc
     5221 gtactccggg tgagcggcgc ggcaacaatc gccggcgagg atataaccaa cgaaagcata
     5281 accacaacgg tgaccgaggc gttgagattg tctgccgatg ccgtcgccac ctcgatttac
     5341 gtcggcactc caaacgagta ctccactata cgcgaactgg cccggctgtg cgacgactgt
     5401 gaaaccttcg gccttccggt gctggccgta accgcgatcg ggaaggaccg ggagcgcaaa
     5461 accgatccga gatacctctc gctggccgtg agggttgccg tggagatggg cgccgatatc
     5521 gtcaaaacgt attattgcga cgaaaaattc gagaaggtca tcgaggccgc tgccggcata
     5581 cccgtagtca tagccggcgg tccaaagatg gattcggtgc tggatgtcct gaagatcgcc
     5641 gaaaatgcca caagggcggg agctcgcggg gtcgatatgg gtagaaacgt ttggcagcac
     5701 gagagaccgg tggagatgct gctggctctg aaggatatag tcaacgacgg catgcccgcc
     5761 gaagaggctt attcgaaacg cttcgggaga tagtcatgaa agttgttgag atgatctcgg
     5821 tcaggaagga gtttcccggg gtcgttgctc ttgacaatat cgacttcgct ctggaagaag
     5881 gggagattca tggactggta ggtgaaaacg gggccggaaa gtccacgctc ataaacatac
     5941 tcgccggagt ctttcagcag acttcgggag agctcagact gttcggcaag ccggtaaaat
     6001 tgagcagtcc ttccgaggcg cttcacaggg gcgtaggggt agtttatcag gacaccgttc
     6061 tggatccgtt ttttactgcc gaggagaaca tctggctggg gagggaacct tcccggcttt
     6121 cggtgttgaa aggcagggag atcagatcca acacgaagag gctttgcgag aagtacggaa
     6181 tcgaagtgcc gctgcgtgtt ccggttggaa ctctaagtgt tgccaagcag aagatggtcg
     6241 agatcctcag ggcgctgagc atgaactcaa aagtactggt tctagacgag ccgacggcct
     6301 cgataaccca gagcgatacg gataatcttt tcgagatcct aaaaaacctg aaaaagtccg
     6361 gcataagcat catcttcgtc tcccaccacc tggacgaagt cttcaatctc tgcgatagaa
     6421 tctccgtcct cagaaacggt cgatacgaag gcacatccgt ctcttcggag ctatcgacac
     6481 gcgacctcat aaagctgatg accaacaggg atctggtaga tcaatatccc aagcaagctt
     6541 cggagatcgg agagggaatt ctcaaggttt cgaagctcaa cgtagcgaga ctgggattga
     6601 aggatatctc cttcgaggtg aagaggggag agatagtggg cttcttcgcc t
//
LOCUS       DAOOLY010000127         6640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13747_length_6640_cov_4.484282, whole genome shotgun sequence.
ACCESSION   DAOOLY010000127 DAOOLY010000000
VERSION     DAOOLY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13747_length_6640_cov_4.484282"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLD15_07310"
     CDS             complement(<1..534)
                     /locus_tag="PLD15_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPB63750.1"
                     /translation="MEVKGTWTVKKGFAEMFKNGVIMDVTNPEQARIAQEAGAVAVMA
                     LERVPADIRKEGGVARMAKIGLIKEIMESVSIPVMAKARIGHIAEAKILEAIGVDFID
                     ESEVLTPADDRYHIDKRLFTVPFVCGARNLGEAVRRISEGAAMIRTKGEAGTGNIIEA
                     VKHMRTVNEEVRKVQMMN"
     gene            complement(524..1852)
                     /locus_tag="PLD15_07315"
     CDS             complement(524..1852)
                     /locus_tag="PLD15_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPB63751.1"
                     /translation="MEKLRLDPRVFKVPIDKMRAGYYSDKYFTRLVEVLKCANKSTRV
                     LYQFFPRRDATIVGIDEALAILRFGTGYYADEGRAKGLFEEILEVEKQINHAAWDMDR
                     EALVELSSWKWDLKMKLNDLWQDRWEELDVEALYDGDEARDMEPIMTVEGDPRNFGYL
                     ETILLGVIARASSTATAVKSVVKAARGKEILFFSARFDHFWTQATDGYAALKAGAFGV
                     STDANADYWGVESMGTIPHALIAVYGGDTAQAALEFDKYIDSKVNRIVLVDWNNDVIG
                     TTYDVVAKAYTALTGREFTLGQTDPSPVIGTGKGRVWGVRFDTSGSLRDKSVVPKDRS
                     SLGVCPELVWRAREEFDKNGMHDLKIIVSGGFTAEKIDLFEKLEVPADVYGVGSSLLK
                     DKLDFTADIVEVDGRPCAKVGRGKGDLSRLSRVESDYWNDNKKRGVEHGS"
     gene            complement(2040..3008)
                     /locus_tag="PLD15_07320"
     CDS             complement(2040..3008)
                     /locus_tag="PLD15_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63752.1"
                     /translation="MKFGGIVAIFLGLLIILSVTHVLPSFSFAVEIFLAIVFLYNGFK
                     VFRRFKAEHMGSLIFGAILVVDLLIGFNIIKNFRGWGFWELVVAMIGSYIVGWGIVVL
                     FRKSFFRVGKEPSSTRQTINVSRPEEFGELDVELEANLTKVLLMDNSSNGIDANISFD
                     KLSFNGNLQYNMDKNRATLKAKCKAKAGVSSVLSKSRMNMEISSRPLLRVEANLDGAD
                     TVLDFSNLNLDRARVKTSLSRLSIIPSTLRDCIIDIDCEVTSLNIRVPKDVGISIVHE
                     GELNWSNFNNLMERERGYVSTNIDRASSTCQINVKSDMSKISIDWI"
     gene            complement(3005..4138)
                     /locus_tag="PLD15_07325"
     CDS             complement(3005..4138)
                     /locus_tag="PLD15_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPB63753.1"
                     /translation="MEKKFEFDSAGIEALKVSAASADLQVELVEQGNIVAFVSVSSSD
                     YVPEADKSGSKLHIRFQRGVNIGIPFIKNLFDVQAKVENIRLQVPRNILELDLNNASG
                     DIKLADFELRMLKVSNVSGDTIIESCSAAEFNLNTVSGDLSFVASNYRRGKFNSVSGD
                     LTIKGLPPMERETNVSSVSGDVYVSYSGDPKVDAAVSSVSGDINSDIKLIKVEKKHYR
                     TSEGQPQEYLKMSSVSGDLIISTAYGGKPAREVKQKDDGEPVVAKKTEKASSIEENLQ
                     DRETNKIVRLYEEGKLTRNYAFEMLSILGYSSVEIEDMLRVDEEARRARKNEESGYEE
                     SEDGKMMEAVEEINDKNEWHEPPQVPPAPEQPEPPEKKEGDEK"
     gene            complement(4150..4512)
                     /locus_tag="PLD15_07330"
     CDS             complement(4150..4512)
                     /locus_tag="PLD15_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63754.1"
                     /translation="MDKAEVTKILKLVADGKISPEQGTDLLGEFVETYGTTGSRRKFN
                     IEVFNMNSGKIEANIKLPVKLASFVGNFIKEKDANFKIGNENIDMNIKEVLEEVIKTG
                     ESVEIEADGRRITIRIED"
     gene            complement(4521..4895)
                     /locus_tag="PLD15_07335"
     CDS             complement(4521..4895)
                     /locus_tag="PLD15_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HPB63755.1"
                     /translation="MKYSITNCPVCGGKMTITEYKCDSCGTSIRGRFELDDIMKLSPE
                     QLDFLKVFIKNRGNLSEVQKELDISYPTARNRLEDIVRGMGYQVVDKDREEASKILEK
                     LESGELQPDEALEMLRKVRKKK"
     gene            complement(5185..5877)
                     /locus_tag="PLD15_07340"
     CDS             complement(5185..5877)
                     /locus_tag="PLD15_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPB63756.1"
                     /translation="METITERKLDPMISGDNSNEAFNAISHLLAALLALAGLVLLIVF
                     SAIERKWMHLVSFSIYGFSVFVSMTLSSILHFFLWFKRYFKIFGILDHSAIYLLIAGT
                     YTPFCLVVVKGGLGWSIFGIIWGLAIFNIVLKAVFFSRMPLWLSMGGYLLMGWLSLSM
                     VYSVYIRLGIWSILLMLMGGLFYTIGAVIFITEKPNPFPGKIAHHELWHILVMLGNTT
                     FLLMMFLYVLPF"
     gene            complement(5991..>6640)
                     /locus_tag="PLD15_07345"
     CDS             complement(5991..>6640)
                     /locus_tag="PLD15_07345"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB63757.1"
                     /translation="TGIVGIGRDITDIKRTEGALKRSWDQLILTMSRMLRIKDPYTAG
                     HQERVKEIAAVVSEELGLSFKSRETVRIASLLHDIGKLAIPADILNKPGKLNEIEWGL
                     IRQHPEQGFELLKDIDFGMPVAEVVRQHHERIDGSGYLFGLKGDEISIEARVLAVADV
                     FEAISSHRPYRASLGVEEAVAELEKNSGVLYDPKVVDALLRVIERGFVFDTGSLE"
BASE COUNT         1454 a         1805 c         1521 g         1860 t
ORIGIN      
        1 gttcatcatc tggacctttc tcacttcttc gttaacggtt ctcatgtgct tgacggcctc
       61 tattatgttt cccgtgccgg cctcgccctt ggtccttatc atggccgcgc cttcggatat
      121 ccttctgacg gcctctccga ggtttctcgc gccacagacg aacggcaccg tgaagagtct
      181 cttatctatg tggtacctgt cgtcggccgg ggtcagaacc tccgattcgt cgatgaagtc
      241 aactccaatg gcttccagta tcttcgcctc ggcgatgtgt cctatgcggg ccttggccat
      301 caccgggatg gagaccgatt ccatgatctc ttttataaga ccgatctttg ccatcctggc
      361 tactccgcct tcttttctta tatcggccgg tactctctca agcgccataa ctgcaactgc
      421 gccggcttcc tgagcgattc tcgcctgctc gggattggta acgtccatga tcacgccgtt
      481 tttgaacatt tcggcgaatc cttttttcac agtccacgtt cccttaactt ccatgttcaa
      541 cacctctctt tttattgtca ttccagtaat cgctttcaac acgggataac ctcgaaagat
      601 caccctttcc ccttccgact tttgcgcagg gtcggccatc gacttccacg atgtccgcgg
      661 tgaagtcgag tttgtccttc agaagcgacg aacccacgcc gtaaacgtcg gccgggactt
      721 cgagcttttc gaagagatcg atcttctcgg cggtgaatcc gcctgagacg attattttca
      781 ggtcatgcat accgttttta tcgaactctt ctctggctct ccagacgagc tccgggcaga
      841 ccccgagcga ggatctgtcc ttgggaacca ccgatttgtc cctaaggctg ccggatgtgt
      901 cgaacctcac gccccagact ctgcctttac ccgtacctat aacgggcgaa ggatctgtct
      961 ggcccagagt gaattctctc ccggtgaggg ccgtgtaagc cttcgccacg acatcgtaag
     1021 tggtgcctat cacgtcgttg ttccagtcaa ccagtactat cctgttcact ttagagtcta
     1081 tgtatttgtc gaattccagg gctgcctgtg ccgtgtcgcc accgtagacg gctattaggg
     1141 cgtgcggtat ggttcccatg ctctcgacgc cccagtagtc ggcgttggcg tcggttgata
     1201 cgccgaaggc accggctttg agagcggcat atccgtcggt agcctgggtc cagaagtgat
     1261 cgaaacgggc gctgaagaag aggatctctt ttccccttgc agccttgacc accgatttca
     1321 cggccgttgc agttgaagag gcccttgcaa tgacaccgag aagtatagtc tccagatatc
     1381 cgaagttgcg cgggtcacct tccacggtca ttatcggttc catatcccgg gcttcatcac
     1441 cgtcgtacag agcttcaaca tctagctctt cccatctatc ctgccagaga tcgttcaatt
     1501 tcatcttgag atcccatttc catgaggaaa gctcgacaag ggcttcacga tccatgtccc
     1561 atgcggcgtg gtttatctgc ttttcgactt cgagtatttc ttcgaaaagc cctttggcgc
     1621 gtccctcatc ggcatagtag ccggttccga accgcagtat tgccagcgct tcatcgatgc
     1681 cgacgatggt ggcatccctt ctgggaaaaa actggtacag cactctggtg ctcttgtttg
     1741 cgcatttcaa aacttcgaca agcctcgtga aatatttgtc ggagtagtag ccggccctca
     1801 tcttgtcgat ggggactttg aacactctgg gatcgagcct gagcttctcc atagatgccc
     1861 cctgcatatt tatgtgaatc gccttcgact caacatcaaa acacaccgga aaagagcccc
     1921 ggtaacctga ttatataata ataagatagt ttcggcttgt tggttttgtt aattgcaacg
     1981 ggtcggagcg atccaaaaga aaaccggcct tacggccggt ctccttcgtt gttattccat
     2041 catatccagt cgatggaaat cttcgacata tccgacttga cgtttatctg gcaggtagat
     2101 gaagcacgat cgatgttggt cgatacgtag cccctttctc tctccataag attgttgaag
     2161 ttgctccagt tgagttcgcc ctcgtggact atgcttatgc cgacatcctt cggaactctt
     2221 atgttgaggg aggttacctc acaatcgatg tctattatgc aatctctgag cgtcgatgga
     2281 attatggaga gtctcgatag ggaggttttc accctggccc tgtcgaggtt gaggttcgaa
     2341 aaatccagca ccgtgtcggc tccatccagg ttcgcctcca ctctcagaag aggcctggag
     2401 cttatttcca tgttcattct cgatttcgaa agaacgctgg aaacaccggc tttagcctta
     2461 catttggctt tcagagtcgc tctgttcttg tccatgttgt actgcaagtt gccgttgaag
     2521 ctcagtttat cgaaacttat attcgcatct ataccgttcg aagagttgtc cataagcaga
     2581 accttggtca ggttggcctc taactccacg tcgagctctc cgaactcttc gggacgggag
     2641 acgttgatag tctggcgcgt cgaggatggc tctttgccta ctcgaaaaaa gctctttctg
     2701 aaaagcacca ctattcccca tcccactatg tatgaaccga tcatcgcaac caccagttcc
     2761 cagaaacccc atcccctgaa gttctttata atgttgaagc cgatcaaaag atctacgacg
     2821 agaatcgctc cgaagatcag gctacccatg tgttcggcct tgaatctcct gaagactttg
     2881 aaaccgttgt acaggaaaac tatggccaaa aagatctcaa cggcgaagga gaagctaggt
     2941 agcacgtggg tgaccgacag gattatgagt aaccccagga agatcgccac tatcccgccg
     3001 aacttcactt ttcatcacct tccttttttt ctggtggttc tggttgctcg ggagctggtg
     3061 ggacttgagg aggctcgtgc cattcgtttt tatcgtttat ttcttcgacg gcctccatca
     3121 tcttcccgtc ttcgctttct tcatagccgg attcttcatt ttttcgggcg cgccttgcct
     3181 cttcgtcgac ccggagcata tcttctattt cgaccgacga atagccaaga atcgaaagca
     3241 tctcgaaggc gtaattcctc gtcagcttgc cttcttcgta caatctgact atcttgttgg
     3301 tctcccggtc ttgaaggttc tcctcgatgc ttgaggcctt ctcggtcttt ttcgccacca
     3361 ccggttcgcc gtcatccttc tgcttaactt cccttgcggg tttgccgcca taggctgtgc
     3421 ttattataag atcgccggaa acgctgctca tcttcaggta ctcctgtggc tgaccttcgc
     3481 tggtgcggta gtgctttttc tcgactttta tcagcttgat atcggaattt atatccccgc
     3541 tgaccgagga aactgcggca tcgaccttag gatcgcccga gtaagacacg taaacgtcgc
     3601 cgctgaccga tgaaacgttg gtctctcgct ccatgggggg caatcctttg atcgtgaggt
     3661 ctccggatac ggagttgaac ttccccctgc ggtagtttga agccacgaaa gagagatcgc
     3721 cgctcacggt gttcaagttg aactcggcgg ccgagcagct ctcgatgatg gtgtcgccgc
     3781 tcacgttgct gactttgagc atccggagtt cgaagtcggc cagtttgata tcccccgagg
     3841 cgttgttcag atcgagttcg agtatgtttc tgggtacctg gagccgtatg ttttcaactt
     3901 tagcctgcac atcgaagagg ttcttgatga acggaatgcc tatattgaca cccctctgaa
     3961 atcttatatg gagtttactg ccggacttgt cggcttcggg aacgtaatcg ctcgaagaga
     4021 cgctcacgaa ggccacaata ttgccctgct cgacaagctc gacctgaaga tcggcactcg
     4081 cggcactgac cttcaaagct tctatgccgg cgctgtcaaa ttcgaatttc ttctccatga
     4141 tattccccct caatcctcta tccttatcgt tattctcctg ccgtccgctt ctatttcgac
     4201 gctttctccg gtttttatta cttcttcgag cacttctttg atgttcatgt cgatattctc
     4261 gttcccgatc ttgaagttgg catccttttc tttgatgaaa tttcccacga aactggccag
     4321 cttgaccgga agttttatgt tcgcctctat tttaccgctg ttcatattga acacttcgat
     4381 gttgaacttt ctcctggacc ctgtcgtacc atacgtttcc acaaattctc caagaaggtc
     4441 cgtgccctgc tcgggagaaa tctttccgtc ggccacgagc ttcaggattt tagtcacttc
     4501 agccttgtcc atactatcac ctacttcttc tttcttactt ttctgagcat ttcaagagct
     4561 tcgtcgggct gcaactctcc cgattcgagt ttctcgagga tcttggaagc ctcttcgcgg
     4621 tccttatcca ccacctggta gcccattccc cgcactatgt cttccagcct gttccttgcc
     4681 gtcgggtacg agatatccag ttccttctgg acttcggaga gatttccccg attcttgata
     4741 aagactttca aaaaatccag ctgttcgggc gagagtttca ttatatcgtc cagttcgaac
     4801 ctccccctta tactggttcc acaactatcg cacttgtact ccgtgatcgt catcttcccc
     4861 ccacagacgg gacagttggt tatagagtat ttcatcgatg cacctccaaa acatgagtcg
     4921 attcaacaat tgttgtcaac tgtgttcatt atacaactta aaacttgaaa agtcaaatat
     4981 gtaattaaat atctttaagt acacttcaat ttattcgaag catcgttgag taatttacat
     5041 cgatacattg aaatccccgt ctaagagttt ttttcgcgct tagtactgaa gagtttcaag
     5101 ttgtctgaga agcagattct cctggggaag taccggaact agatagattt aatggattat
     5161 gtaatatctt agataggaga gatttcagaa cggaagcaca tagaggaaca tcatgagaag
     5221 aaaagtggta ttcccgagca tgaccagtat gtgccagagt tcgtggtggg ctatcttccc
     5281 cgggaaggga ttgggttttt ccgttatgaa aatgacggcc ccgatcgtgt agaatagtcc
     5341 gcccatgagc atgaggagaa tagaccatat ccccagtctg atatacacgc tgtacaccat
     5401 agagagcgat agccagccca tcaggaggta ccctcccatc gaaagccata gcggcattct
     5461 ggagaagaaa acggctttga gcacgatgtt aaagatcgcc agcccccata taataccgaa
     5521 gatggaccag cccaggccac ctttcaccac gaccaggcag aaaggcgtgt acgttccggc
     5581 tatgagcaga tagatcgcac tgtggtcgag aatgccgaag atcttgaaat accgtttgaa
     5641 ccagagaaag aaatgaagga tactgctcag agtcatagat acgaagacac tgaaaccgta
     5701 aatcgagaag ctcacaagat gcatccactt ccgctctatc gccgaaaaga cgatcagcaa
     5761 gaccagcccg gcgagcgcta atagcgcggc cagaagatgg cttatcgcgt tgaaagcttc
     5821 gttggaatta tcgccggata tcatgggatc cagctttctt tccgttatag tctccaaagg
     5881 acttcctttc ttggaaaaga gatgcggtac ttttcaatta taaacggtgt tctcaactta
     5941 cgcgttaaga gagaaactgg gggcagagga cagagacgcg agggcgcctt ttattcgaga
     6001 gagccggtat cgaacacgaa gcctctttct attactctca gcagcgcatc gaccactttc
     6061 ggatcgtaca gaacccccga gttcttctcc agttcggcca cggcttcttc cactcccaga
     6121 gacgccctgt atggtctgtg gctcgatatg gcttcgaaga catcggcgac tgccagcacg
     6181 cgcgcctcga tcgatatctc atcgccttta agcccaaaca ggtatcccga tccatcgatt
     6241 cgctcgtggt gctgacgaac cacttctgcg acgggcattc caaaatctat atccttgagt
     6301 aattcgaagc cctgttcggg atgttgcctg atgagccccc actctatttc gttcagcttt
     6361 cccggcttgt tgagtatatc ggctggtatc gccagctttc ctatgtcgtg aaggagcgaa
     6421 gctatcctca cggtttcccg gctcttgaaa gagagtccca actcttcgga aaccaccgcc
     6481 gctatttctt tgactctctc ctggtgaccg gccgtgtacg gatcttttat ccttagcatt
     6541 cgcgacatgg tcagtatcaa ttgatcccag cttctcttga gagccccttc ggtccttttt
     6601 atatcggtta tatctctccc gatgccgact attcccgtta
//
LOCUS       DAOOLY010000128         6640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13750_length_6640_cov_3.945786, whole genome shotgun sequence.
ACCESSION   DAOOLY010000128 DAOOLY010000000
VERSION     DAOOLY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13750_length_6640_cov_3.945786"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLD15_07350"
     CDS             complement(<1..499)
                     /locus_tag="PLD15_07350"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB63758.1"
                     /translation="MAELISGYFAQSGFTNLSWENIVMFAIASLLMYLAVAKDAEPLL
                     LIPIAFGMIIANIPPQVTGVFEPGTGFMWILQQGLVLGIYPPLIFLGIGAVTDFSFVL
                     ANPKTLFLGAAAQIGVFVTFIGANLLGFSLLALKGDKKAQLITWVREHKNTLLMMEGL
                     SLILFA"
     gene            complement(565..3207)
                     /gene="ppdK"
                     /locus_tag="PLD15_07355"
     CDS             complement(565..3207)
                     /gene="ppdK"
                     /locus_tag="PLD15_07355"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPB63759.1"
                     /translation="MSRKYVYYFGKTKAEGDAKMKNLLGGKGANLAEMVRLGFPVPPG
                     FTISTEVCKYYYDHGEKYPGELTGEVKAAMEMLEKETGKGFGSAERPLLVSVRSGAAI
                     SMPGMMDTILNLGLNNETVTGLINESGNPRFCYDAYRRFLQMFGDVVLKIEHSEFEKA
                     LASVKKEKGVKLDTELDATDMEEVVKRYLEVYKKAGKEFPQDPWEQLWMSTNAVFGSW
                     MNERAIKYRALNGVKEGDLLGTAVNICTMVFGNTGDDSGTGVAFTRDPNTGERKFYGE
                     YLPNAQGEDVVAGTRTPYPMTRLHEINPAAYNQLLDIFEKLEKHYRDMQDIEFTVEHG
                     TLYLLQTRNGKRTPYASVKIAVDLVKEGLISKEEAIMRVTPEHIETLLHPYFTNETLE
                     SAEKLAKGIAASPGAASGVIAFDPETAERMVKEEGKVVILVRPETSPEDIAGMAAAQG
                     ILTSTGGKTSHAAVVARGMGKTCIVGCEAIEVDEHAKEMKVNGKILKEGDWLSIDGTT
                     GEVFMGKLDSVKPQGLEGPLAEILVWADKIRKLGVRTNADTPKDASIARGFGAEGIGL
                     TRTEHMFFQEDRIFAMRQMITSNTLEQRKKALSKLLPMQEDDFYGIFKAMEGYPVTIR
                     LLDPPLHEFLPKEDEDVRDLAKEMGMGYYELKATIEELHEFNPMMGFRGCRLAVVYPE
                     IAEMQTRAIIGAAIKLVKEGMNVIPEIMIPLIIELEELKYVKAIIVKAADEMIREAGV
                     DLVYKVGTMIEVPRAAITADEIAREAEFFSFGTNDLTQMTFGFSRDDYGKFVSHYLEK
                     GILPTDPFQKLDRVGVGQLVKMGTEKGRSTRPDLKVGICGEHGGEPSSVEFCHMVGLN
                     YVSCSPYRVPIARLAAAQAVIKNR"
     gene            complement(3361..4530)
                     /locus_tag="PLD15_07360"
     CDS             complement(3361..4530)
                     /locus_tag="PLD15_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63760.1"
                     /translation="MKKYFHSFNFYAFSFSVLLYLLSSSINVKGAEWGLSYIEIGLIN
                     FGGSIFYVSMASTLGRIGDKIGFKKSLSIGMIAMAVFLNLGFFWRGVMDITLFAFGMN
                     LFFGFFFPSLEGLISKGERREGIDPLTTVIRFVLSWSAGNIVGMALGPYLIQKMPKVV
                     FMFGILLSLMSSVMIFRSRKKWGESLPGPYSEKLKKPMPQADLPKIKEYRRSYRLAFL
                     LAGIIYTSGMALFPKLISASGLPLENIGFLTVGANVGVFLSFIFMSRFKFWIGNPGKA
                     LILMMVIFSTAVLSFFMPQTAPVFFAVTFLCGTTYAVPYIFAIFYGLNSQENDHGKQG
                     GVHESMVGIIFGVGPLIGGYFLQVWPDLKSVGVMTLILISVIFINQIRFIRKVKR"
     gene            complement(4600..5862)
                     /locus_tag="PLD15_07365"
     CDS             complement(4600..5862)
                     /locus_tag="PLD15_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63761.1"
                     /translation="MRKSILLVVMFAILAVFVMAKTEIEFWHAMGGAQGTTVTEIVNS
                     FNEANPDIEVKAIYVGNYNALQQKLLVSAESNTLPALSQAYGNWTARLIPRGVVQELN
                     TLIADPEAGLTDEEWAAIWSPFKRMVTWGSTIYAVPFNKSTYVLYYNTDLFEQYGLEV
                     PKTMEDLLFAAMMLTEDKNKDGTIDQYGFGFRTTIDHFMTFLRANKGEAVEILNDGSV
                     KVIINSPQAKEALQLMYDMAHTYGVALFQGGYLDAPFGDGQIAMFIETIASASYVDSG
                     SRGKHGWAWAPVPMWEVQAPPFAGTDVVMFAGITAEQKVAAWKFMKYLVSPEIQAYWS
                     VKTGYMPVTQLALDTPQWKAYEQQNPTVAVPISQIPFGSVDPNVALWDDVRNVLGTMV
                     GDVINQKRTVDEGIAWAEQELLRIIANQ"
     gene            complement(5905..6471)
                     /gene="coaE"
                     /locus_tag="PLD15_07370"
     CDS             complement(5905..6471)
                     /gene="coaE"
                     /locus_tag="PLD15_07370"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730308.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPB63762.1"
                     /translation="MLGVAGKAGSGKSTIASALEKSGAIRVSLDEIGHQALDSMKEEI
                     RREFGEFVMTGDSVDRKKLGNAVFISPELLERLNRIVHPVIRSVALAKMSDDAKLYVI
                     DGALLHEIGLSEFCDKILWVECSPEETVRRLALRGMTECKARSILQSQQHLDRIRDSA
                     DAVVDTSGDAATTMRKVEEVLFEWGIML"
BASE COUNT         1570 a         1744 c         1542 g         1784 t
ORIGIN      
        1 ccgcaaaaag gatcagggat aaaccttcca tcatcagaag cgtgtttttg tgctctctca
       61 cccaggtaat tagctgcgct tttttatctc cttttagagc cagcaatgaa aaaccaagaa
      121 gattcgcccc gatgaacgtc acaaagaccc ctatctgcgc cgccgcaccc aggaaaagag
      181 tcttgggatt tgcaaggacg aacgaaaaat ctgtcacagc tccgatcccg agaaaaataa
      241 gaggaggata gattcccaga accagaccct gctgcaggat ccacatgaaa cccgttcccg
      301 gctcgaaaac accggtcacc tggggaggaa tgttggcgat tatcatccca aaagctatcg
      361 gaataagcaa tagaggctcc gcgtctttcg ctacggcaag gtacattaga agcgaagcta
      421 ttgcgaacat gactatgttt tcccagctca gatttgtgaa accggactga gcgaaatacc
      481 ctgagatcaa ctctgccatg ataggctaac ctccagaaaa ataatgaaag ccgcgacccc
      541 gcgcgagagt cacggcgaaa gtaattatct gtttttgatg accgcctgtg cggctgccag
      601 ccttgctatc ggtacacggt agggtgagca gctcacgtag ttgagaccaa ccatgtggca
      661 gaattccact gaggaaggtt ctccaccgtg ttcaccgcag atcccgactt tcaggtccgg
      721 tctggttgaa cgacccttct cggtacccat tttgacgagc tgtcccacgc caaccctgtc
      781 gagtttctgg aaggggtcgg tgggaagaat acccttttca agataatgac ttacgaactt
      841 tccatagtcg tctctgctga acccgaatgt catctgggtt aaatcgttgg tgccgaagct
      901 gaagaactcg gcttccctgg cgatttcatc ggcagtgata gccgcccttg gcacctcaat
      961 catggttcca actttgtaaa ctagatcgac accggcctcc cttatcattt catcggccgc
     1021 ttttacgatt atggctttga cgtacttgag ttcttccagc tcgattatga ggggtatcat
     1081 tatttctggg attacgttca taccctcttt aacgagcttg attgcggctc ctatgattgc
     1141 tctggtctgc atttcggcga tctccggata aactaccgcc agtctgcagc ccctgaaacc
     1201 catcatcggg ttgaattcgt ggagctcttc aatagtcgcc ttcaactcgt aatatcccat
     1261 tcccatctct ttggccaggt ctctcacgtc ttcgtcttct ttcgggagaa attcgtgtag
     1321 agggggatcg agaagtctta tagtaacggg atagccttcc attgccttga agatcccgta
     1381 gaaatcatct tcttgcatag gaagcagttt agaaagggct tttttgcgct gctcaagagt
     1441 gttggaagtg atcatctgac gcatggcaaa tatcctgtcc tcctggaaga acatatgttc
     1501 cgtcctggta agaccgatac cttccgcgcc aaagcctctc gcaatcgaag cgtctttagg
     1561 tgtatcggcg ttggttctga cacccagctt tcttattttg tcggcccaga ctagtatttc
     1621 agctagaggt ccctcgagtc cctgcggctt cacggaatcg agctttccca tgaagacctc
     1681 gcccgtagta ccgtctatac tcaaccagtc tccctctttg aggatctttc cgttgacctt
     1741 catttctttg gcatgctcgt caacttcgat tgcttcgcat ccgactatac aggtcttgcc
     1801 cattcccctg gccacaacgg cggcatggga tgtcttgcca ccggtagagg taaggatgcc
     1861 ctgcgcggcg gccattccgg cgatgtcctc tggagaggtt tccggtctga cgagaataac
     1921 cacctttccc tcttctttga ccattctctc ggctgtctcg gggtcgaacg cgataactcc
     1981 agaagctgct cctggagaag cggcgatgcc cttggcaagt ttttcagcgc tttccagggt
     2041 ttcgttagtg aagtagggat ggagaagagt ctcgatatgt tcgggagtga ctctcattat
     2101 ggcttcttcc ttggatatta gtccttcttt aaccagatca acggcaatct tcacactcgc
     2161 gtagggtgtt ctctttccat tccttgtctg aaggagataa agcgtaccgt gttcgaccgt
     2221 gaattcgata tcctgcatgt ccctgtagtg cttttccagt ttttcgaaga tatcgagcaa
     2281 ctggttgtaa gcggccgggt tgatctcatg aagcctcgtc atcgggtaag gagtcctggt
     2341 accggcaact acgtcttcac cttgcgcgtt tggcaggtac tctccgtaga attttctttc
     2401 acccgtgttg ggatctcttg tgaaggcaac gcctgtaccg ctgtcatccc cggtgtttcc
     2461 gaaaaccatg gtgcatatgt taacggccgt tccgagaaga tcaccctcct tcactccatt
     2521 gagcgccctg tatttgatgg ctctttcatt catccagctt ccaaaaacag cattagtcga
     2581 catccaaagc tgctcccatg gatcctgagg gaattctttg ccggcctttt tgtagacttc
     2641 caggtatctc ttaaccactt cttccatatc cgtggcatcc agttcggtat cgagtttcac
     2701 gcccttctct tttttaacgc tggcaagagc cttttcaaac tcggaatgct ctatcttgag
     2761 cactacatct ccgaacatct gaaggaacct tctgtaagcg tcgtagcaga acctgggatt
     2821 tcccgattcg tttatgagcc ctgtgactgt ctcgttgttg aggcccaggt taagaatcgt
     2881 atccatcatt cctggcatgg agatggccgc tcccgacctc acagatacca gaagcggtct
     2941 ttcggcagag ccaaagcctt tgccggtttc tttctcgagc atttccattg ccgctttgac
     3001 ctcgccggtg agttcaccag ggtatttttc accgtgatcg tagtagtact tgcagacttc
     3061 tgtagagatc gtgaagcccg ggggtaccgg gaatccgagt ctaaccattt cggccagatt
     3121 ggctccttta ccgcccagga gattcttcat cttcgcgtcg ccctcggctt tcgtcttacc
     3181 aaaatagtac acatatttcc tcgacacttt gtttcctccc ttcctgaaaa ctattcgagg
     3241 cgattataac acagcctttc acgggtgatg tttagaatga aatcactgcc gtgctttaag
     3301 tacgttcaag gatcttcgct gactaacagt gaagtattcg aaatactttc gattgaagag
     3361 tcaccttttg acttttctta taaagcgaat ctgattgatg aatatcaccg agatgagtat
     3421 gagcgtcatg acgcccacgc ttttcaaatc gggccatact tgaagaaaat accctcctat
     3481 caacgggccg acaccgaaga ttatgccgac catggattcg tgcactcctc cttgcttgcc
     3541 atgatcgttt tcctgtgagt ttagaccgta gaagatcgcg aaaatgtagg gcaccgcgta
     3601 agtggtgccg caaaggaaag tcacggcgaa gaacactgga gccgtttggg gcatgaaaaa
     3661 tgacagcacg gctgtcgaaa aaattaccat catcagtatc agggccttac cgggattgcc
     3721 gatccagaac ttgaatctac tcatgaaaat aaaagagaga aagactccga cgttcgcacc
     3781 aaccgttaaa aatcctatgt tctccagagg tagaccggag gccgaaatca actttggaaa
     3841 gagcgccatt ccgctcgtat atattatccc tgctaggagg aaggccagtc tgtaagatct
     3901 tcgatactct tttatttttg gaaggtcggc ttgcggcatc ggctttttga gcttttcact
     3961 gtaaggtccg gggagcgatt cgccccattt tttcctgctt ctgaaaatca tcaccgaact
     4021 cataagggag agaaggattc caaacatgaa gacgaccttc ggcatctttt gaattagata
     4081 aggaccgaga gccataccga cgatattacc ggcgctccag gacaggacga agcgtatcac
     4141 cgtcgtcaaa gggtctatac cttctctcct ctctcccttg gagatcaaac cctcaagaga
     4201 cggaaagaaa aagccgaaga agaggttcat cccgaaggcg aagagagtta tatccatgac
     4261 acctctccag aaaaaaccca gattcagaaa aacggccata gcgatcattc cgatggagag
     4321 actctttttg aagccgattt tatctcctat cctgcccaaa gtgctggcca tcgatacata
     4381 aaagatactc ccaccgaagt ttatcagccc gatctctatg tatgaaagcc cccattcggc
     4441 tcctttaacg tttatggatg aggatagtaa atacaaaagt accgaaaagg aaaaagcgta
     4501 gaaattgaag gaatgaaaat actttttcac tgacccatac ctcgtcaatt aaacaataaa
     4561 aaagggaggc gactgcctcc ccagatttta ctacactttt tattgattgg ctataattct
     4621 tagcagttcc tgctctgccc aggctatacc ttcatcaacc gttctcttct ggttgatgac
     4681 gtcgcccacc atcgttccga gtacgtttct cacatcatcc cagagagcaa cgttcggatc
     4741 gacagaaccg aaggggatct ggcttatcgg aacggcaacg gtcggattct gctgttcgta
     4801 tgccttccac tgaggtgtat cgagggcaag ctgcgtaacc ggcatgtagc ctgtcttcac
     4861 cgaccagtaa gcctggatct ccgggcttac caggtacttc atgaatttcc atgcggcgac
     4921 cttctgttcg gcagtgatgc cggcaaacat tactacatcc gtcccggcga acggtggtgc
     4981 ctggacttcc cacatcggaa ccggtgccca cgcccagccg tgctttcctc tggaaccgct
     5041 gtcaacgtaa gaggctgacg ctatagtttc gatgaacata gcgatctgac cgtccccgaa
     5101 cggagcgtcg agataaccac cctggaagag agcgacaccg taggtgtgtg ccatatcgta
     5161 catgagctgc agagcttctt ttgcttgcgg agagttgatt ataaccttga ccgaaccgtc
     5221 gttgagtatt tcgacagctt cgcctttgtt ggccctgagg aaggtcatga aatggtctat
     5281 ggtcgttctg aaaccgaatc cgtactggtc gatggtcccg tctttgttct tgtcctcggt
     5341 aagcatcatg gccgcgaaga gcaggtcttc catcgttttt gggacttcaa ggccgtactg
     5401 ttcgaagaga tcggtgttgt agtagagaac atatgtactc ttgttgaagg gcacggcgta
     5461 gatcgtgctt ccccaggtca ccattctctt gaaaggcgac cagatggcgg cccattcttc
     5521 gtcggtcaga ccggcttcgg gatcggcgat aagggtattc agttcctgca cgacgcccct
     5581 ggggatcaat ctggctgtcc agtttccgta agcctgagag agggccggaa gggtgttact
     5641 ttcagcactc acgaggagtt tttgctgcaa tgcattgtag ttacccacgt agatagcctt
     5701 gacttcgata tccgggttgg cttcgttgaa cgaattcacg atctctgtaa cggtcgttcc
     5761 ctgtgcgcca cccattgcgt gccagaactc gatttccgtc ttcgccatta cgaagactgc
     5821 gagaatcgca aacatcacta ctagaagaat acttttgcgc atctctttac ctccttattc
     5881 aaagtgtttc aagcgtcgaa gtaattatag cattatgccc cattcaaaaa gaacctcttc
     5941 cacttttcgc atagtcgtgg cagcatctcc agatgtgtca acaacagcgt cggcagagtc
     6001 tcttatgcgg tcaagatgtt gctgggactg taagatcgat ctggctttgc attcggtcat
     6061 tccacgtagc gcgaggcgcc gcaccgtctc ttctggggag cattccaccc acaagatctt
     6121 atcgcaaaat tccgaaagac caatttcgtg aagcagcgcg ccatcgatta cgtaaagctt
     6181 ggcatcgtcg ctcattttcg caagtgccac agaacgaatc actggatgaa cgatacggtt
     6241 caacctttcc aggagttctg gacttatgaa gacagcattt cccagttttt ttctatccac
     6301 cgaatctccg gtcattacaa attctccgaa ctctctgcga atttcctctt tcattgaatc
     6361 gagcgcctgg tggcctattt cgtcgaggga tactcggatc gcaccggact tttcgagcgc
     6421 cgaagcaatc gtagatttgc cagaaccagc cttaccggct acgcccagca ccgtcaaatc
     6481 aatctctcac ctttctccat gacgtttatt accacttttg agatctcgct gggtgaaaac
     6541 ttaagggcct ctttctcatc gggagtgagg gttatgatat cggcttcaat ctcttccatt
     6601 tcgccgattt tcttcagcaa cgactctgat tgcgcggaga
//
LOCUS       DAOOLY010000129         6609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13842_length_6609_cov_4.890143, whole genome shotgun sequence.
ACCESSION   DAOOLY010000129 DAOOLY010000000
VERSION     DAOOLY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13842_length_6609_cov_4.890143"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..845)
                     /locus_tag="PLD15_07375"
     CDS             complement(120..845)
                     /locus_tag="PLD15_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB63763.1"
                     /translation="MKEVSVPVYYRIYKELKQRIARGTYSVKLPTEKNLCDEFNVSRL
                     TLRRALEELKRESIIEASKGRGTFVLEEKHEEKIGTLTGFTEEAKRDGREATSLVLKN
                     TLVAPEEDVAELFEIPSGGMVVVLERVRYLDGEPYGMERAFLNPMVDIRILNVVQRDM
                     SKESLYYIMKSELGIVLDHAQETIEVCRMTRDEARHLQVKEGNFAIARERHTYTDHGL
                     CVEMVKSVYRGDRYRLKVVRRVE"
     gene            complement(842..1753)
                     /gene="murQ"
                     /locus_tag="PLD15_07380"
     CDS             complement(842..1753)
                     /gene="murQ"
                     /locus_tag="PLD15_07380"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPB63764.1"
                     /translation="MMKFEKLETERSNPRTRNIDELPTLEMLRLMNEEDATVPLSVRD
                     VLAEIASAVDLCAGAIENGGRIIYAGAGTSGRLGVLDAAEVVPTFGVSKDLFVTLMAG
                     GNDALTTPVEQAEDSEALGASEVEKTSITAKDVVVGITASGRTPFVGGILKYARKKGA
                     RTILVCNVGEPGLKDHADVTIAVRTGPEVITGSTRLKAGTAQKMVLNMISTITMVKVG
                     RVFGNYMIGVKILNEKLVDRATRIVSEISDLPYGESAKILEESGRDVPLAILMALTGA
                     KREECEKILKKNRGNIRLSLKELGGVS"
     gene            complement(1756..3294)
                     /gene="nagZ"
                     /locus_tag="PLD15_07385"
     CDS             complement(1756..3294)
                     /gene="nagZ"
                     /locus_tag="PLD15_07385"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPB63765.1"
                     /translation="MNLDQAVGRLFLIGLPGKEMDSETEEILKDIKPGAVILFAKNIE
                     SPVQVRTLLEDINRVLGYPLAVAIDQEGGIVTRLREGFSVSPGAMALAATGSVENVSL
                     ASGIMAREMRALGVNWNLAPVVDINCNPKNPGIGVRSFGDDPEKVVKYATAFVQAMKR
                     EGVMSCLKHFPGKGRVEVDAHIDLPTLDVPIATMLSSELRPFREIPGDSIMPSHIYMP
                     QMQAKRVPASMSREILIELARNMLGYRGVLVADDLGMGGVSNYFSPEEAAVEGLRNGM
                     DFLTFCHVPEVQKRAKKAVIKAVEQSGELTGRLEESLERVETFRRKATAMKLEPLNVI
                     GTDESLSTMQRISDASITAILEDESLVPLSLEAVTAIYSVRLSRLVQVEDGPLKGVPA
                     VARELAQMSDCPLIDFDGKISVEEAIILADKAPRKGVRIVFTENAHLSAGQREFLMQL
                     SRRPGRTLLIALRNPYDAFIKGVKNSILSYGYETLSQKSLLKVLTGEIKPQGVLPVEI
                     PQEV"
     gene            3573..4592
                     /locus_tag="PLD15_07390"
     CDS             3573..4592
                     /locus_tag="PLD15_07390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPB63766.1"
                     /translation="MIWNRVDDTVLSGIGKAVYPGAALVVGKDNEMLYSKCYGTIAVD
                     DNRPVDLETVYDLASMTKIVATTTAVMLLVSRGEVSLWDTLGDFFEVPESKKNIALYH
                     LLTHTSGMQPYSEAWRELRGRELLESIIRLEPLSGLMEKIDYSCLNFITLMAVVEKVT
                     CRKYDEFCLNEIFKPMGMDNTTYLPPLAHSIAPTCERDGVTLRGLPDDELAYYLGGVS
                     GNAGLFSTAGDLFKFVSGLMYARLIPSRVFESFVGETVLIGEDKRHLGWMSPSKGSSG
                     GDMLGEDAFGHTGFTGTSLWVDPQSGLYVILLTNRTNISRRETIPQMQQIRRRLHNLV
                     FGGLG"
     gene            4589..5704
                     /locus_tag="PLD15_07395"
     CDS             4589..5704
                     /locus_tag="PLD15_07395"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPB63767.1"
                     /translation="MTWSNFGEILSKESRNIVGVMSGTSADGLELALTRCAGSGKKMK
                     VELLKHSSVAFDRAFQDEVVATYNPSTSGVDRVNRMNFLIGRTHARHIKSFLNSLAAP
                     VDAIAYHGQTVYHDPADGSTLQIGEADFVSHETGLPVIFDFRKKDMVVGGEGAPLIPY
                     LDWILFEEGTYAVNLGGIANVTYVSESFDGVIAFDSGPANCLIDLTVKKYFNVDYDRD
                     GLIASRGKIDGSILETLIERDRTYFLRKPPKSTGREIYNETFLEGITHGKPEDLVRTL
                     VRFTVHQLVESIRSYLSLGNEIVVTGGGALNPVMMEDLKTLSGLKVIVPDRAFLQAKE
                     AMGMAVLAQEFLNGVGANVPSVTGARQRVVLGKLALP"
     gene            5744..>6609
                     /locus_tag="PLD15_07400"
     CDS             5744..>6609
                     /locus_tag="PLD15_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63768.1"
                     /translation="MKRILLVILVSLCAVMVLAAPFVEDTLLTPDKNPKMGGTLYLAL
                     TGAPQSFNFYGTLDNNAYTVAGQMLTGLVEAHPVTSAIRPALAESWEVSENNKEVIFH
                     LRDVNWSDGAPLVADDVIFTFKYFIMNPVARGNSVDRFTIESQPIEWVKIDDKTVKAI
                     LPAPYAAFFTVLSHAYIYPSHALDSKIDKSRPDSVNDLWLTNTPPSEIPANGPFILSE
                     YIVDQKVVMTRNPNYWKVDVFGNKLPYVDRIEYLVVSDAQVRLAKFMAGELDYMAVSG
                     ADFPILKERELSG"
BASE COUNT         1489 a         1849 c         1664 g         1607 t
ORIGIN      
        1 actgacggcc gtcagattca ctccggtccg gtaactcttt ctgagctgtc catctccgga
       61 aatagccgta accctgaggt gcgtaccgcc tccgtccata gatatcacgc ttttgatact
      121 cattcgactc tcctcaccac ttttaacctg taacggtctc cccggtaaac cgacttgacc
      181 atttccacgc aaagcccgtg atcggtgtat gtatgacgtt ctctggctat cgcgaagttt
      241 ccttccttta cctgaagatg cctggcctca tctctggtca tccggcagac ttctatagtc
      301 tcctgcgcgt ggtcgagcac gatacccagt tcacttttca ttatgtaata gagagactcc
      361 ttcgacatat ctctctgaac gacattcaaa atccgtatat ccaccatcgg attgagaaag
      421 gccctctcca tgccgtaagg ttcaccgtcc agatacctca ctctctccag aaccactacc
      481 atgccgcccg aagggatctc gaagagctcc gcaacgtcct cttcgggagc tacgagcgta
      541 tttttcaaca cgagagaagt ggcctctcgt ccgtcgcgtt tcgcctcctc ggtgaatccg
      601 gtgagggtcc ctattttctc ttcgtgcttt tcctccagca cgaaggttcc gcgtcccttc
      661 gaagcctcga tgatcgactc tctcttgagc tcttccagag ccctccttag ggttagtctg
      721 ctgacgttga actcgtcgca gaggttcttc tccgtgggca attttaccga gtaagttccc
      781 ctcgcgattc gctgcttgag ctctttgtag atacggtaat agactggaac ggaaacttcc
      841 ttcaactaac tcctccaagc tctttcaagg aaagccttat gtttccccgg tttttcttca
      901 agatcttttc gcactcttct cttttggcgc ccgtgagagc catcagtatc gccagcggca
      961 catctcttcc agactcttcc agtattttag cagattctcc atacggcagg tcgcttattt
     1021 ccgaaacaat ccttgtggca cggtcaacga gcttttcgtt gagaatcttc actcctatca
     1081 tatagtttcc gaagactctc cccaccttaa ccatggtgat cgtgctgatc atgttgagga
     1141 ccatcttttg ggccgtgccg gccttcagtc gggtgctacc ggttatcact tcagggccgg
     1201 tgcgaaccgc gatcgtgaca tccgcgtgat ctttcaatcc gggctccccg acgttacaga
     1261 cgagaatcgt cctggctccc ttttttctgg cgtatttcaa tatacccccg acaaagggag
     1321 ttcttcccga ggccgttata ccgacgacaa cgtccttcgc ggttatcgag gtcttttcca
     1381 cctcgctggc gccgagagct tcgctgtctt cggcctgttc taccggagtt gtcagcgcgt
     1441 cgttgcctcc ggccataagg gttacgaaga gatcctttga aaccccgaaa gtgggaacga
     1501 cctccgcggc gtcgagaacg ccgagccgac cgctcgttcc cgcaccggcg taaattattc
     1561 tccccccatt ttcgatggcg ccggcgcaca ggtcaacggc cgaagcgatt tcggccagca
     1621 catctctcac cgaaagggga acggtggcat cctcttcgtt catcaatctg agcatctcca
     1681 acgtaggcag ttcatctata tttctcgttc tgggatttga cctttccgtt tcgagcttct
     1741 cgaacttcat catgcctaga cctcctgagg aatctctact ggaagaaccc cttgcggttt
     1801 tatctccccc gtaagtactt taagaagact cttctgtgag agcgtttcgt aaccgtagct
     1861 gagaatgctg tttttcacac cctttataaa agcgtcgtac ggatttctca gtgctataag
     1921 gagcgttcta cccggacgac gcgacagctg catgaggaac tcgcgctgac cggcggagag
     1981 gtgtgcgttc tccgtaaaga cgatcctgac ccctttcctt ggggctttgt cggcgagtat
     2041 tatcgcttcc tctactgaga tcttcccgtc gaaatctatc agagggcagt cggacatctg
     2101 cgccagttcc ctcgcgacgg ccggaactcc ttttagcggt ccgtcctcta cctgaaccag
     2161 tctggacagc ctcacggagt agatcgccgt aacagcctca agagaaagcg gaaccagaga
     2221 ttcatcctct agaatggccg tgatcgaagc gtcggaaatt ctctgcatcg tcgagagact
     2281 ctcgtcggtt ccgataacgt tcagaggttc gagcttcatg gccgtcgcct ttctcctgaa
     2341 ggtttctaca cgctcgagcg attcttcgag ccttccggtc agttccccgg attgttcgac
     2401 ggctttaatg accgccttct tcgcgcgttt ctggacttcg gggacatggc agaaagtgag
     2461 aaagtccatc ccgtttctca atccctcgac cgcggcttct tcgggggaga agtagttgct
     2521 gacccctccc atgccgagat cgtcggccac cagaacaccc ctgtagccca gcatatttct
     2581 agccagttcg atcaaaatct ccctcgacat cgacgcggga accctttttg cctgcatctg
     2641 tggcatatat atgtgggaag gcataatcga atcgccgggt atctccctga aaggcctcaa
     2701 ctcactggaa agcatcgtcg cgatcggaac gtcgagagtg ggcagatcga tgtgagcatc
     2761 tacttccacc cggccctttc cgggaaagtg cttaaggcag ctcataacgc cttccctttt
     2821 catagcctgc acgaaggcgg tggcgtattt caccactttt tcgggatcgt ctccgaaact
     2881 tctaacgccg attcccgggt tcttcggatt gcaatttata tcgaccaccg gcgcgagatt
     2941 ccagttgact cccagggctc tcatctctct ggccattata ccggaggcca gagacacgtt
     3001 ctcgacgctc ccggtggcag ccagcgccat ggcaccgggc gagaccgaga agccctccct
     3061 caaccgcgtc acgataccgc cttcctggtc tatggcgacg gcgagcggat atcccagaac
     3121 cctgttgatg tcctcaagaa gagttcttac ctggacgggg ctttctatgt tcttggcaaa
     3181 gagaatcacc gcacccggtt ttatatcttt aagtatctcc tcggtctcac tgtccatttc
     3241 ttttccgggg agccctataa ggaaaagtct tccgactgcc tgatcgagat tcataaagtc
     3301 gctttctcct tctatactca tatacatcca tattctatgg cttaatcact tgtcttgtca
     3361 aataactatt tgtatagtat gaatatacat aacaactatc cggtgccctc atcgacttta
     3421 atcgcccgta gataccggca atgacagaaa aaaagggttg ttactatatg ccgttttgag
     3481 ttagcatagg cagtgacggg acaattatat ttcgccggaa gttgaactat actgttattc
     3541 aggtacgaat ctattcacag ggaggtatca ctttgatctg gaaccgtgtt gacgatacag
     3601 tattgtcggg aatcgggaag gcggtctacc cgggagcggc cctggtggtc ggcaaggaca
     3661 acgagatgct ctactcgaag tgttatggaa cgatcgcggt agacgacaac aggccggttg
     3721 acctggagac tgtgtacgat ctggccagca tgaccaagat agtcgcgacc acgactgccg
     3781 tgatgttgct ggtatccaga ggtgaagttt cgctctggga cacgctggga gacttcttcg
     3841 aagtgcctga atccaagaaa aatatcgcgc tgtaccacct cctaacccac acgtccggca
     3901 tgcagcccta ttcggaggcc tggagggagc tgagaggaag ggaattgctg gagagtataa
     3961 tcaggctcga gcctctgtcc ggtctgatgg agaagatcga ctactcctgc ctgaatttca
     4021 taacccttat ggccgtggtc gagaaggtca cctgcagaaa gtacgacgag ttctgcctga
     4081 acgagatctt caaacccatg ggcatggaca acacaaccta cctccccccg ctcgcccatt
     4141 ccatagctcc gacctgcgag cgtgacggag taaccctgag gggactcccc gacgacgaac
     4201 tggcctatta cctgggaggc gtcagcggaa acgcgggact tttttccacc gccggcgatc
     4261 tcttcaaatt cgtatcgggc cttatgtacg ccagactcat accatcgaga gtcttcgaga
     4321 gtttcgtcgg ggagaccgtc ttgattggcg aggataagcg ccatctgggc tggatgtctc
     4381 cctcgaaagg ttccagcggc ggcgacatgc tgggagagga tgcgttcggc cacacgggtt
     4441 tcacgggcac ctctctatgg gtcgatcccc agagcggcct gtacgtgata ctgctgacga
     4501 accggaccaa catcagcaga agagagacca ttccacagat gcaacagata aggcgtaggt
     4561 tgcacaacct tgtttttgga ggtcttggat gacctggagt aatttcggcg agatactttc
     4621 gaaggaaagt agaaatatcg tcggcgtcat gagcggtacc tcggccgacg gcctggaact
     4681 ggcgctcacc agatgcgccg gttcgggcaa aaagatgaag gtggaactgt taaagcactc
     4741 gtccgtcgcc ttcgacaggg cttttcagga cgaagtcgtg gccacgtaca acccttccac
     4801 ctcgggtgtc gacagggtca acaggatgaa ctttctcatc ggcagaacgc acgcccggca
     4861 cataaaaagc tttctgaact cgcttgccgc ccctgtcgat gctatcgcct atcacggcca
     4921 gacggtgtac cacgatccgg ccgacggctc tacgttgcag ataggtgagg ccgatttcgt
     4981 ctcgcacgag accgggctgc cagtgatctt cgatttcagg aaaaaggaca tggtggtggg
     5041 cggcgaaggc gctccactca taccctatct ggactggatt ctcttcgaag agggcactta
     5101 cgcggtcaac ctcggcggca tagccaacgt cacgtacgtc tcggagagtt tcgacggcgt
     5161 gatcgccttc gacagcggac cggccaactg tctgatcgac ctcaccgtaa aaaagtactt
     5221 caacgtcgat tacgacaggg acggtctcat agcttcgaga ggaaagatag acgggagtat
     5281 tcttgaaaca cttatagaaa gagatagaac ttactttttg agaaaaccac caaaatctac
     5341 gggaagagag atatacaacg aaacctttct tgaaggtata actcatggaa agccggagga
     5401 tcttgtcagg actctagtaa gattcactgt tcatcagctg gtggagagta tcagatctta
     5461 tttgtctttg gggaatgaga tagtcgttac cggaggcggt gccctcaatc ccgttatgat
     5521 ggaggatcta aagaccctga gcggtctgaa agtgatcgtt cccgatcgcg catttctcca
     5581 ggcgaaggaa gccatgggaa tggccgtgct cgcccaggag tttttgaacg gggtcggagc
     5641 gaacgttcca tcggtaaccg gtgcgagaca aagagttgtt ctgggcaagt tagctttacc
     5701 atgatctccc agacgggaga gtttatacaa aggaggtttt agcgtgaaac gtatcttatt
     5761 ggtgattctc gtatcgctct gtgctgtgat ggttctggcg gctcccttcg ttgaggacac
     5821 actgctcacg cccgacaaga acccgaaaat gggtggaacg ctgtacctgg cgctaacggg
     5881 ggctccgcag tcgttcaact tctacggtac gctggacaac aacgcctaca ccgttgccgg
     5941 ccagatgctc acaggtctcg ttgaagctca cccggtgaca tcggccatca ggccggcgct
     6001 cgccgagtcc tgggaagtat cggagaacaa caaggaagtc attttccacc tcagagacgt
     6061 caactggtca gacggcgcac cgctggtcgc cgacgacgtc atcttcacct tcaagtactt
     6121 cataatgaac ccagtcgcga gaggaaactc cgttgaccgc ttcaccatag agtcgcagcc
     6181 gatcgaatgg gtgaagatag acgacaaaac cgtaaaagcc atactcccgg cgccttacgc
     6241 cgctttcttc acggttctgt cgcacgccta tatctacccc tctcacgcgc tcgatagcaa
     6301 gatcgacaaa agcagaccgg attccgtcaa cgatttatgg ctgaccaaca caccgccgag
     6361 cgaaatcccg gccaacggtc ccttcatact gtccgagtac atagtcgatc agaaagttgt
     6421 aatgaccagg aacccgaact actggaaagt agatgttttc ggcaacaaac tgccttacgt
     6481 ggacagaata gaatacctgg ttgtcagcga cgctcaggta agactggcaa agttcatggc
     6541 aggagagctc gattacatgg ccgtatccgg ggcggacttc ccgattctca aggaaaggga
     6601 gctttccgg
//
LOCUS       DAOOLY010000130         6608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13848_length_6608_cov_5.213948, whole genome shotgun sequence.
ACCESSION   DAOOLY010000130 DAOOLY010000000
VERSION     DAOOLY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13848_length_6608_cov_5.213948"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLD15_07405"
     CDS             complement(<1..365)
                     /locus_tag="PLD15_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63769.1"
                     /translation="MSLVDYPGKVALTVFTSSCNFDCAYCQNETLKRRVSEELSEETV
                     IDYLDKRGALIDAIVITGGEPTLRTGELIPFIGRLRERVPGKLVKLDTNGWNPALLAR
                     LMERVDFVAMDLKSLDYSL"
     gene            complement(307..2460)
                     /locus_tag="PLD15_07410"
     CDS             complement(307..2460)
                     /locus_tag="PLD15_07410"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPB63770.1"
                     /translation="MLNVRKRNGSIVEFDLNKIINAMKKAFDATRTHYNEDILERVAL
                     RVVADFQPKVSDNLVDIEDIQDSVERTLENLGFAEVAKAYILYRTHREKLRELKSSML
                     DFSETVNSYLNMLDWRVRENSTVTYSIGGLILHNSGTVTANYWLREMYDPQIGNAHRE
                     GDIHIHDLSMLSGYCAGWSLRQLIEEGLGGVRGKVSSSPARHLSTLVNQMVNFLGILQ
                     NEWAGAQAFSSFDTYLAPFVKIDNLSLKEVKQNIQSFVFGVNTPSRWGTQAPFTNVTL
                     DWTVPKKMRDEPAIVGGKRLDFTYGDCQEEMDMINRAFLEILDEGDANGRGFQYPIPT
                     YNITKDFDWENENAKLLFEITSKYGTPYFQNFINSDLDPDDVRSMCCRLQLDKRELKR
                     RGGGLFGSDEFTGSIGVVTINLPRISYLSKNETEFLERLSRVMELASRSLEVKRSVVE
                     KLNGEGLYPYTRRYLKNFDNHFSTVGLVGMNEACLNARWIGKTIAENEGYNFSLRVLD
                     FMRNKIMTFQETTGHLYNLEATPAESTSYRLAKIDKKKFPQIITAGKDSPYYTNSSHL
                     PVDFTSDIFSALDLQETLQQKYTGGTVFHAFLGEKISDWQTTRELVRKIAGNYRIPYF
                     TLSPTYSVCQNHGYLSGEQYSCPKCGAATEVYSRITGYYRPVQNWNIGKQEEFKRRRV
                     YQIRRESIDELRRLGKSESGRLSRESSPDGIYLKL"
     gene            complement(2689..3243)
                     /locus_tag="PLD15_07415"
     CDS             complement(2689..3243)
                     /locus_tag="PLD15_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB63771.1"
                     /translation="MKIAGMEFVVLDTETTGFDVNRGARLLEVAGVRIRDWRIVEEDF
                     FDSLIDPGCIVPITITGLTGISNLTVKGMPPVEKVLRDFFRFVEDSILVIHNAPFDLS
                     FLNHFGERCGLGRLSNTYIDTIEMSRSLFRRSRNNLDILLARLGIVADSRHRALGDAM
                     ATARAFLAMSEKMGKESVTRFVRK"
     gene            3372..3692
                     /locus_tag="PLD15_07420"
     CDS             3372..3692
                     /locus_tag="PLD15_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63772.1"
                     /translation="MDDKVNHTEEEIRERYRKAIESSLQKAPVRTGERIYLTDLWAVT
                     SIPEEIIIEILSSCEVKLPEEVRCIVDDRRKKKRVLYGEKESNEQGGDPKQPRIRKVQ
                     GEDS"
     gene            3613..4686
                     /locus_tag="PLD15_07425"
     CDS             3613..4686
                     /locus_tag="PLD15_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB63773.1"
                     /translation="MERKSQMNRVAILSSPEYAKCREKIAEGFDLLNFDDDVREKKVL
                     LKPNLLSARTPDSSVTTHPEIVRAVAEIFLRRGAKVSVGDSPARVSTITAARAAGIES
                     VCRELDIPLVDFDDPVSVVLQEGTYRNFEIARPVLEADLVVNLAKLKTHSLTLVTFAV
                     KNLFGCIPGARKQGWHFRARNMGDFSRMLLDLARYLKPSLTVLDGVEGMDGNGPSNGR
                     KIYPGIVALSRNVFSLDDAIAELFQVKHSKVPLLRMAREENLVGEYEIVGDGPVPARL
                     VLPETNYIAVYGAGFLRHFVTKFPRIDRKNCIECRNCEKACPVGAIEIDRFRIDYSKC
                     ITCYVCHEICPENAIVFKRRFHI"
     gene            4703..6163
                     /gene="glpK"
                     /locus_tag="PLD15_07430"
     CDS             4703..6163
                     /gene="glpK"
                     /locus_tag="PLD15_07430"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPB63774.1"
                     /translation="MSYILAIDQGTSSSKAVLFDEEFYQVAVSQEEFRQIYPNPGRVE
                     HDPRDIILSTMSSVENVVSDARVSYREIEAIGITNQRETVVAWDAITGQPLCNAIVWQ
                     DRRTTERCKELREKFGEKIHLKTGLMPDPYFSATKMEWMLQNVPGVREAAKRRTLRFG
                     TIDSWLVWNLTREKRYVCDYSNASRTMLFNIRELKWDGELLDMFGIDEEFLPKVVDSC
                     SPLGASIYGPEIAGIAGDQQASLFGQTAFSRGDAKCTYGTGSFILMNTGSEPKYSRHG
                     LLTTIGWKLGKDTVYAIEGSIFASGALITWLKEGLGIIANPSETESLAREVQDNGGVY
                     FSGALTGLGAPYWDASARGTLIGLTRGTKKGHIIRAVLEYMAFRTREILELMEEETGI
                     KLKKLLVDGGVTRNNFLMEMQANVLGIPVDRSLIKETTALGAAMFSGLCTGLWDIEVL
                     KNRRISEVEFEPNGNRMEEEFSKWKEVLRRSMNWAR"
     gene            6121..6597
                     /gene="tsaE"
                     /locus_tag="PLD15_07435"
     CDS             6121..6597
                     /gene="tsaE"
                     /locus_tag="PLD15_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB63775.1"
                     /translation="MERSTEKIYELGQMKIDQLLNLAGKIGERLSGGETILLYGDLGT
                     GKTTFVRGLARGLRIEERLVRSPTFNIINTYPGRLILVHADLYRLEDRRELLDLDLEG
                     LGDYNTVMAVEWPEMYRPFVEGPCLIVRLEHWSENERVVSLSSMDVEADEILSFLN"
BASE COUNT         1593 a         1612 c         1810 g         1593 t
ORIGIN      
        1 aagagcgagt aatccagcga cttcagatcc atagctacga aatctacacg ctccataaga
       61 cgggcaagaa gagccgggtt ccagccgttg gtgtcgagtt taaccagttt gcccggcacc
      121 cgctccctca gacggcctat gaagggtatc agctctccgg tcctcaaagt cggctcgccc
      181 ccggttataa cgatggcgtc tatcagagct ccgcgcttat cgagataatc tatcacggtt
      241 tcttcgctta attcttcgct tactcgcctc ttgagcgttt cgttctggca gtaggcacag
      301 tcgaaattac agcttgaggt aaataccgtc agggctactt tccctggata atctaccaga
      361 ctcactcttt cccagcctgc gaagttcatc tatgctttct ctccttatct gataaaccct
      421 tctccttttg aactcttcct gtttgccaat gttccagttc tggactgggc ggtaatatcc
      481 tgtgattctt gaataaacct ccgttgcggc gccgcatttc gggcacgagt actgctcgcc
      541 gctcaggtaa ccgtggttct ggcaaacgga ataggtgggg gacagggtaa agtaaggtat
      601 tctgtaattg cccgctattt ttctcactag ttccctggtt gtctgccagt cagatatctt
      661 ctcgcccaga aaagcgtgga agaccgtgcc gcccgtgtat ttctgctgga gagtctcctg
      721 aaggtcgagc gcggagaaaa tatcgctcgt aaaatccacc gggaggtgcg aggagttcgt
      781 atagtagggg ctgtcctttc cggcggtgat tatctggggg aacttctttt tgtcgatctt
      841 ggccaggcgg taagaggttg attcggccgg agtcgcctcg aggttgtaca ggtgcccggt
      901 ggtctcctgg aaggtcatta ttttgtttct catgaagtcg agcactctca gggagaagtt
      961 gtacccctcg ttttcggcta tagtcttccc gatccagcgt gcgttgagac aggcctcgtt
     1021 cattccaacc agcccgacgg tcgagaaatg attgtcgaag ttcttgagat atctgcgggt
     1081 gtaagggtat aggccctcac cgttcaattt ctccaccacc gaccgcttca cctctagact
     1141 cctgctggcc agttccatca ccctggacag tctttccaga aactccgttt cgttcttgct
     1201 gagatatgag atcctaggaa ggttgatcgt caccactccg atcgagccgg tgaattcgtc
     1261 cgagccgaac aatccccctc ccctcctctt gagttcgcgt ttatccagct gcagcctgca
     1321 gcacatgctt ctgacatcgt cgggatcaag gtcggagttt atgaaattct gaaaataagg
     1381 tgttccgtat ttactcgtga tttcgaagag aagcttcgca ttttcatttt cccaatcgaa
     1441 atctttcgtt atgttgtaag tagggatcgg gtactggaag ccccgtccgt tggcgtcgcc
     1501 ctcgtcgagt atctccagga aggcccggtt tatcatgtcc atctcttcct gacagtctcc
     1561 gtaggtgaaa tcgagtctct tccctcccac tatggccggt tcgtctctca tctttttcgg
     1621 caccgtccag tcgagcgtta cgttggtgaa gggtgcctgc gttccccatc tggaaggcgt
     1681 gttgacgccg aagacgaagg attgtatatt ctgtttcact tccttcagag agaggttgtc
     1741 tatcttcacg aagggagcca gatacgtgtc gaaagacgag aaggcctggg cgccggccca
     1801 ttcgttctgc aagataccca ggaagttcac catctgattc accagagtcg aaagatgtct
     1861 cgccggtgaa gaggatacct tgccgcgcac ccctccgagc ccctcctcaa tcagctgtct
     1921 caaactccaa cccgcgcagt agccagatag catagaaaga tcgtgaatgt gaatatcacc
     1981 ctcgcggtgg gcgttgccta tctggggatc gtacatctcc ctcaaccagt agttggccgt
     2041 caccgttccg ctgttgtgaa ggattagacc acctatggaa taggtcacgg ttgagttctc
     2101 tctgacacgc cagtccagca tgttgaggta gctgttgact gtttccgaga agtcaagcat
     2161 cgaggatttg agttctctga gcttttcgcg gtgagttctg tagagaatgt aagccttggc
     2221 gacctccgcg aacccaagat tttcgagcgt cctttcgacc gagtcctgga tatcttctat
     2281 atctacgagg ttgtcgctca cctttggctg aaaatcagcc acgacacgca gcgcaaccct
     2341 ctccagaatg tcttcgttgt agtgggtcct tgtggcatcg aaggctttct tcatggcgtt
     2401 tattattttg ttcaggtcga attctacgat tgatccattt ctttttctga cgttcagcat
     2461 catcatacct ccatgaataa gctcaataaa tagtggacga taaacattat accacaatat
     2521 atagtgaatc cgaagtcgaa acgggggtcg attttggcag ataacggcga ttggctccgt
     2581 ttagattcta ccagatcgaa atgttggggt ttttaagttt ctgtgggcgg attctttccg
     2641 tacggattct ctggaaatca ttgggccata ccttttcaga accacggcct acttccttac
     2701 gaatctcgtt acactttctt tacccatctt ctcgctcata gcgaggaagg cgcgagcagt
     2761 cgccatcgcg tcgccaagcg ctctgtgtct ggagtccgcg actattccca acctggcgag
     2821 aagaatatcc agattgttcc ggctacgtct gaagagactt cgggacattt cgatggtatc
     2881 tatgtaggtg ttggaaagtc ttccaagacc gcatctctca ccgaagtggt tgaggaaaga
     2941 cagatcgaag ggagcgttgt gaatgaccag tatcgaatct tcgacgaagc gaaagaaatc
     3001 tcgaagaacc ttttcaactg gaggcatgcc cttcacagtt agattgctta tcccggtcag
     3061 gcccgtgatc gtgatgggaa cgatgcagcc gggatcgata aggctgtcga agaaatcttc
     3121 ctccactatt cgccagtcgc ggatcctcac accggctacc tcaagtaacc tggcaccgcg
     3181 attgacgtca aagcccgtcg tttcggtgtc gagaaccaca aactccattc ctgcgatctt
     3241 catctggcac ttccctccct tccttttaaa attttacaac aagaacgaac acacggtcca
     3301 gaacacaacg ggagcgttta ttcatttgcg agtgatgcgt atagagtata attctactga
     3361 ggtgactttc catggatgat aaggtgaatc atacagaaga ggaaatcaga gaaaggtaca
     3421 ggaaggctat cgaatcatcc ctgcaaaagg cgcccgtgag aacgggcgaa aggatatatc
     3481 tcacagatct ctgggccgtt acatcgatcc cggaagagat aataatagag atactctctt
     3541 cttgcgaagt gaagcttccc gaagaggtgc gctgcatagt ggatgatcgt agaaagaaga
     3601 agagggttct ttatggagag aaagagtcaa atgaacaggg tggcgatcct aagcagccca
     3661 gaatacgcaa agtgcaggga gaagatagct gagggcttcg atctgctgaa ttttgacgac
     3721 gatgttcgag aaaaaaaggt actgctgaag cctaacctgc tctcagcaag aacgccagac
     3781 agctcggtga cgacccatcc cgaaatagtc cgcgcggtgg cggagatttt cctccggcgc
     3841 ggggcaaagg tcagtgtagg agattccccg gccagggtca gtaccattac cgcggccaga
     3901 gcagcaggga ttgaaagcgt ctgcagggaa ctggacatac cccttgtaga tttcgacgat
     3961 cccgtttctg tggttctgca ggaggggacg tacaggaact tcgagatagc ccgcccggtc
     4021 ctggaggccg acctcgtagt caatctggcc aagctcaaga ctcattcgct cacgctcgtc
     4081 acgttcgcag tcaagaacct cttcggctgt atccccggtg caagaaagca gggctggcat
     4141 ttcagagcga gaaacatggg ggatttttcc aggatgcttc tggacctggc gaggtatttg
     4201 aagccctcgc tgacggtgct ggacggagta gaggggatgg acggaaacgg accgtcgaac
     4261 gggaggaaga tctatcccgg aatcgtggcg ctctcaagaa atgtcttttc tctggacgat
     4321 gcgatagcgg aactcttcca ggtgaagcat tcgaaagtcc cgcttttgag aatggccagg
     4381 gaagagaatc tggttggcga gtacgaaatt gtgggcgacg gacccgtgcc ggcgagactg
     4441 gttctgcccg aaacaaacta catcgcggtt tacggtgccg gtttcttgag gcatttcgtc
     4501 acgaagtttc cgaggataga caggaagaac tgcatcgagt gcaggaactg cgaaaaggcc
     4561 tgcccggtcg gggcgataga gatagacagg ttcaggatcg attatagcaa gtgcataacc
     4621 tgttacgtct gccacgagat ctgtccggag aacgcgatcg ttttcaagag acgttttcat
     4681 atatgaagga ggcggcgtta ggatgagtta cattctggcc atcgatcagg gaaccagcag
     4741 cagtaaggcc gtactattcg atgaggagtt ttatcaggtg gccgtttcac aggaggagtt
     4801 caggcagata taccccaatc ctggcagggt cgaacacgat ccgagagata ttattctcag
     4861 taccatgagt tctgtggaaa acgtggtatc cgacgccagg gtctcttacc gtgagatcga
     4921 agccataggt ataaccaacc agcgcgagac agtggtcgca tgggatgcca taaccggcca
     4981 gcctttgtgc aacgctatcg tctggcagga taggagaacc acggaaagat gcaaagagct
     5041 gagggaaaag ttcggggaga agatacacct caagactgga ttgatgcccg atccgtattt
     5101 ctcggccacg aagatggaat ggatgctgca aaacgttccc ggagtccggg aagccgcgaa
     5161 gaggcgcacc ttgcgtttcg gcacgatcga ctcctggctg gtctggaacc taaccagaga
     5221 gaagcgatat gtgtgcgatt actcgaacgc ttcaagaacg atgctcttca atatacggga
     5281 gctgaagtgg gacggcgaac ttctcgacat gttcggaata gatgaagagt tcctgccgaa
     5341 ggtcgtggat tcctgcagcc ctctgggagc ttcgatatac ggtccggaga tagccggtat
     5401 agccggagac cagcaggctt cgctcttcgg acagacggcc ttctcgcggg gcgatgccaa
     5461 gtgcacctac ggcaccggct ctttcatact catgaatacc gggtcggagc cgaaatactc
     5521 aaggcacggc cttcttacaa cgataggatg gaaacttggg aaggatactg tctacgccat
     5581 agaaggatcg atcttcgcct ctggggccct tataacttgg ttaaaagagg gtctgggaat
     5641 aatagccaat ccctcggaga ctgaatcgct tgcgcgtgag gttcaggaca acggcggagt
     5701 ttacttctcg ggagctctga ccggtctggg ggcgccgtac tgggatgctt cggccagggg
     5761 gacgctgata ggactgacca gagggacgaa gaagggccat ataatcagag ccgtgctgga
     5821 atacatggcc ttcaggactc gcgaaatact ggaactgatg gaagaggaga cggggataaa
     5881 actgaagaag ctcctggtgg acggcggagt caccaggaac aacttcctga tggagatgca
     5941 ggccaacgtg cttggcatcc ctgtagatag atctctgata aaggaaacaa cggcgctggg
     6001 tgctgcaatg ttctcgggcc tttgcacggg cctgtgggac attgaggtgc tgaaaaacag
     6061 acgtatcagc gaggtggagt tcgaacccaa cggaaataga atggaggaag agttttcgaa
     6121 gtggaaagaa gtactgagaa gatctatgaa ctgggccaga tgaagataga ccagttgttg
     6181 aacctggccg gcaagatcgg tgaaaggctc tccggaggcg agacgatact gctttacggc
     6241 gatctgggca ccggaaagac gacttttgtg aggggactgg ccagagggct aagaatcgaa
     6301 gagagactgg ttagaagtcc gacgttcaac ataatcaaca cctatcccgg caggttgatt
     6361 cttgttcacg ccgatctcta cagacttgaa gatcgaagag aattactgga tttagatctc
     6421 gaaggtctcg gcgattataa tacggttatg gccgttgaat ggcccgagat gtacagaccc
     6481 ttcgtagaag ggccgtgtct gatagtgagg cttgagcact ggagcgaaaa cgagagagtc
     6541 gtgtccctgt cgtcgatgga cgtggaggcc gatgaaatcc tctctttttt gaactgaaaa
     6601 gccggtat
//
LOCUS       DAOOLY010000131         6568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_13974_length_6568_cov_3.820820, whole genome shotgun sequence.
ACCESSION   DAOOLY010000131 DAOOLY010000000
VERSION     DAOOLY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13974_length_6568_cov_3.820820"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLD15_07440"
     CDS             complement(<1..1185)
                     /locus_tag="PLD15_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB63776.1"
                     /translation="MKVYKRLGEMLVEHGLLSEDDLKQAVAIQKKVGKPLGETLVGMG
                     VISWEDIYGALSRQYGLKVLEEVPNLLSPEILRMIPKPVADRLKVIPIEYLPEINTIR
                     VVTTDVLKVPQIDRELSFLTGSRVNTLLVSPPIFDALYKSSYEESASSEIIEHTFNIE
                     DSEIGIDEEKGQDSEDTPVAKFINSLLDNGIRSDASDVHLEPYEKMAVARLRIDGVLR
                     KVLSYPRKAHNSVVSRIKVMCNMDISDRRFPQDGKFYIRRGGEQFDLRVSTMPTIFGE
                     KVVLRILRVSNARKRLEDLGLSDYNRERFESIINSPYGIILVSGPTGSGKSTTLVAVL
                     NQVTSDRVNVLTAEDPVEYTIEGIAQCQVNSEIGLTFARYLRSFLRQDPDIIMVGEVR
                     DRE"
     gene            complement(1189..2238)
                     /gene="ftsZ"
                     /locus_tag="PLD15_07445"
     CDS             complement(1189..2238)
                     /gene="ftsZ"
                     /locus_tag="PLD15_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPB63777.1"
                     /translation="MSFELDTGKKSKTEIRLPAIKVIGVGGAGGNAVNRMISEGIHGV
                     TFIAANTDIQVLESNKAELKIQLGTELTRGLGAGGNPNVGERAAEESVDEIGTFLEDT
                     DLLFITAGMGGGTGTGAAPIVASIAREMGILTVAVVTTPFFFEGNTRLRTGHEGLRKL
                     KNSVDTLIRISNNKLLQELPPNTSIVDAFAKADETLHHGIKGISELITKRGYINLDFA
                     DVESVLRNAGTAMLGIGVGSGERRAEEAARRALESRLLEKPIDNATGIILNVSAKNIT
                     LREMNIAAAIVRQNCSEDADVKLGLIVDPEMSNDELNITLIAAGLELDEGELMGDASD
                     IPAIYRFGLDINEEE"
     gene            complement(2228..3520)
                     /gene="ftsA"
                     /locus_tag="PLD15_07450"
     CDS             complement(2228..3520)
                     /gene="ftsA"
                     /locus_tag="PLD15_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPB63778.1"
                     /translation="MARGKDYTVSLDVGTNTLKGVVVSREQTGQMVLEAYGSVKTVGL
                     DKGEVKDAVALKQSIQKLIEELTGQMGKKDVEADFRISFTDGDYSVFSENIEEILSEE
                     KQVMVTEKTIQNLMSRLKTEKMKTGNTNIHRSYIRKYILDADKVVFNPVEMYARKLDV
                     EMVFVSSEGKSVEIFRRLFEELFGRGDFLISPALISASEAVLTDTEKQHGVVCVVLGH
                     SFSEIVIYRENLPVYISRIPLGIRHIVLDVARVLGTSVDEAERLLVNYGHCSMYPPDP
                     EDVVEYFGLDERTRKNVSVRRLSTVIYARVKELLNKIRKEIQLFVINNPEYSEERIPG
                     GVVFSGGGSKLRGLTDVGVESLKMPVRIGTYETSFNRRIENSHDVSNDPIFSSCLGNL
                     VSPEEVQGEILEGAVERPKKGFGSFIKSLFFGGVDDEL"
     gene            complement(3527..4165)
                     /locus_tag="PLD15_07455"
     CDS             complement(3527..4165)
                     /locus_tag="PLD15_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4894 domain-containing protein"
                     /protein_id="HPB63779.1"
                     /translation="MSLSLRAAICMVTIMTVWFVAIINFVVESFFTVPLAQFTKIVNP
                     EETVIEFPLAFRRRWKTDIIYPPATVDVSGNRVFLKPGKYIVFYDNSYYWVSDDFVIV
                     DHASMDEITSFPVVGGLNFVLVEGVYKAVDNDIDDFTGAVEGILAERRISGLVTYFDY
                     RNNTLFLRRGITVKVLDWEKLNASKELLLQLENASDRSEYIFLSDGKLLRAR"
     gene            complement(4162..6057)
                     /gene="gyrB"
                     /locus_tag="PLD15_07460"
     CDS             complement(4162..6057)
                     /gene="gyrB"
                     /locus_tag="PLD15_07460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPB63780.1"
                     /translation="MSELYNAQNIKILKGLDPVRKRPGMYIGSTGKSGLHHLVYEIVD
                     NSIDEAMGGFCDRIDITILEDGSVMVSDNGRGIPVDTHPDTGTSALEVVMTTLHAGGK
                     FSKDSYKISGGLHGVGASVVNALSEWMEVRVMTDGKIYRQKYERGKAVTPVDIIGETD
                     INGTVTTFKPDPLVFTVTDFDFDILESRFKELAYLNGGLRLNFEDRRIEESRSYHFEG
                     GIVEFVRALSRSKKTIHKDPIYLEGSFNDVKIQLAMQYTTAYEEETLTFVNNIKTVEG
                     GTHLTGFKTVLTKTMNDLGRKHNILKEKDQNLQGEDVREGLTAILSVFVKEPQFEGQT
                     KAKLGNEEAYEAVIKVVKEKMEEFFDYNQKDLKLILTKAIEAARARLAAKKAREMVRR
                     KNVLENTTLPGKLADCISENIEETEIFIVEGDSAGGSAKMARSRDTQAILPLRGKILN
                     VEKASFDKMLKSETISNIVVALGTGIGEDFEIAGLRYGRIIIMTDADVDGAHITTLLL
                     TLFYRYMKPLITTGKVFVAQAPLYKIEMNRQKHYFYSDEELNAFVEKYPDKKLNYSRF
                     KGLGEMNPEQLWETTMNPTDRKLVQITMEDAEEADRVFTILMGSEVEERRSFIERHAL
                     SISNLDV"
     gene            complement(6074..6367)
                     /locus_tag="PLD15_07465"
     CDS             complement(6074..6367)
                     /locus_tag="PLD15_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPB63781.1"
                     /translation="MKWNHQTLGQIFEGLARRPGLFRKLRVLDLNRDWATIVGESIAG
                     HSSIVDYSDGVLIVAVNDGMWLHELKLRESAFIEKINEAVGSEAVKRIRFRVG"
     gene            complement(6330..>6568)
                     /locus_tag="PLD15_07470"
     CDS             complement(6330..>6568)
                     /locus_tag="PLD15_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPB63782.1"
                     /translation="MERPVASWEMFSADELFLTHTSVGIVPIRKLEEKILIEDFTDGM
                     TRLLLDNFEGYIMTEDSNWSGLDEVEPSDFRADI"
BASE COUNT         1425 a         1802 c         1468 g         1873 t
ORIGIN      
        1 ttctctgtca cggacctctc caaccattat gatatccggg tcctgcctca ggaaggatct
       61 caaataccgg gcgaaggtta ggcctatctc cgagttgacc tgacactgag ctatgccttc
      121 gatggtatat tctacggggt cttcggcggt caaaacattt accctgtcgg aggtgacctg
      181 attcaagacc gcgaccagcg tggttgactt accgcttccg gtcggccccg aaaccagtat
      241 tatgccataa ggggagttta tgatgctttc gaaacgttcc ctgttgtaat cggagagtcc
      301 gaggtcttcg agccttttcc tcgcgttgga gaccctcagg atcctcaaaa cgactttctc
      361 tccgaagatg gtgggcatgg tggaaaccct gaggtcgaac tgttcgccgc cgcgtcttat
      421 gtaaaacttt ccgtcctgag gaaatcttct gtcggagata tccatgttgc acatgacctt
      481 aattctcgaa acgactgaat tgtgagcttt tctcggataa ctcaacactt tcctgagaac
      541 accgtctatc ctgagccggg ccacggccat cttttcataa ggttccagat gaacgtcgct
      601 ggcatcgctt cttataccgt tgtcgagcag agagttgatg aacttcgcga ccggggtatc
      661 ttcgctgtcc tggccttttt cttcatctat tccgatctcg gaatcctcta tgttgaaggt
      721 gtgctctatg atctcgctag aggccgattc ttcgtaggaa gacttgtaga gagcgtcgaa
      781 gatgggcgga gaaaccagca acgtgttaac acgacttcca gtaaggaaag aaagctccct
      841 gtctatctgc ggaactttca aaacatccgt cgtgacgacc cttatggtgt ttatttccgg
      901 caggtattct atgggaataa ctttgagtct atcggccacg ggcttgggga tcatcctgag
      961 gatctcggga gagagcaggt tgggaacctc ttcaaggacc ttgagaccgt actgtcttga
     1021 aagggcgccg tagatatctt cccaggagat cacccccatt cccactagag tctctccgag
     1081 gggtttcccg acttttttct ggatcgcgac ggcctgctta agatcgtctt cgctcaggag
     1141 accgtgttcg acaagcatct ccccaagcct tttatagacc ttcaatgact attcctcctc
     1201 gttgatgtcc agtccaaatc tgtaaatagc cgggatgtcg gaagcgtctc ccatgagttc
     1261 tccttcgtcc agttccagac ctgcggctat cagtgtaata ttcaattcat cgttgctcat
     1321 ttctgggtcg acgatcaagc ccagcttaac gtcggcatct tcgctgcagt tctgtctgac
     1381 tatcgccgcg gctatgttca tctccctgag ggttatgttc ttggccgaga cgttcaggat
     1441 gatacctgtg gcgttatcga tgggcttttc gaggagtctg ctttccaggg cccttctagc
     1501 cgcctcttcg gctctgcgtt ctccagaacc gacaccgata ccgagcattg cggttcccgc
     1561 atttctgaga accgattcca cgtcggcgaa atcgaggttg atgtaacctc tcttggttat
     1621 caattcggag atacctttga ttccgtggtg aagcgtctcg tctgccttgg cgaaggcatc
     1681 gacgatcgag gtgttaggtg gtagctcctg gagtagtttg ttgttggata ttcttatcag
     1741 agtgtccacg gagttcttca gctttctcaa tccctcgtgt ccggtcctca atctggtgtt
     1801 gccctcgaag aagaaaggcg ttgtgacgac ggcgaccgtt agtattccca tctctctagc
     1861 gatcgacgcc acaatcggcg cggcccccgt acctgtaccg ccgcccattc ccgcggttat
     1921 gaatagcaga tccgtatctt cgaggaaagt tcctatctca tcgacactct cctctgcggc
     1981 ccgctctccc acgtttggat ttccaccggc ccccagaccc cttgtgagtt cggtacccag
     2041 ctggatcttc agctcggcct tgttactctc gagaacctgg atatcggtgt tggcagctat
     2101 gaaagtgact ccatgaatcc cttcggaaat cattctatta acggcgtttc caccggcacc
     2161 tccgacgcct attaccttta tggccgggag ccggatttct gtcttgcttt tcttacccgt
     2221 gtcaagctca aagctcatca tcgacacctc caaagaagag ggattttatg aatgatccaa
     2281 aacctttttt aggtctttca actgcgcctt ccaaaatctc tccctgtact tcttcggggg
     2341 acacgagatt gcccagacag gaactgaata tcggatcgtt ggagacatcg tggctgttct
     2401 ctatacgacg attgaaactg gtttcgtagg taccgatcct cacaggcatt ttcagagatt
     2461 caaccccaac atcggtcaac cctctcaatt tcgaaccgcc gccggagaag actactccac
     2521 caggaatcct ctcttcagag tactcgggat tgtttatcac aaacagttgg atttcctttc
     2581 tgattttgtt caacagctct ttcactcttg cgtatatgac tgtagatagt ctcctgacgg
     2641 agacgttctt tctcgttctt tcatcgagtc caaagtattc tactacatct tctggatcgg
     2701 gcggatacat actgcagtgg ccgtagttca cgagcagtct ctcggcctcg tcgaccgaag
     2761 tcccgagcac tcttgccacg tcgagaacta tatgtcttat cccgagaggt attcgggaaa
     2821 tatagaccgg gagattttct ctgtatatca ctatctcaga gaaactatgc cctagaacca
     2881 cgcagacgac accgtgctgc ttttcggtat cggttagaac cgcctccgag gccgatatca
     2941 gtgccggtga tatcagaaag tccccgcggc cgaacagctc ttcgaagagc ctgcggaaga
     3001 tctcgacgga tttcccttcg ctggatacga agaccatctc cacatcgagt tttcgagcgt
     3061 acatctccac agggttgaag accactttgt cggcatctag tatgtatttt ctgatgtaac
     3121 tcctgtgtat atttgtgttg ccggtcttca ttttctcggt cttgagacgg ctcataaggt
     3181 tctgtatggt cttctcagta accatcacct gtttctcttc ggaaagaatc tcctcgatgt
     3241 tttcggagaa aaccgagtag tcgccgtctg tgaaacttat tctgaagtcg gcttcgacgt
     3301 ccttctttcc catctgtccc gtaagctctt caataagttt ttgtatcgat tgctttaggg
     3361 caacagcgtc cttcacctcg cccttgtcga gccccacggt ctttacactc ccgtaggcct
     3421 cgagaaccat ctgtcccgtt tgctctctac tgactacaac acctttcagt gtgttggtac
     3481 ctacatcgag agaaactgta taatccttcc ccctggccat ctagtgtcac ctcgctctca
     3541 acagttttcc atcgcttaaa aagatgtatt cacttctgtc tgaggcgttt tcaagctgaa
     3601 gaagcaattc tttgctcgca ttgagcttct cccagtcaag taccttgacc gttattcccc
     3661 ttctgagaaa caacgtgttg ttcctgtaat caaagtaagt gaccaatccg gagattcttc
     3721 tttcagcaag gataccctca accgccccgg taaaatcatc tatatcatta tcgacagctt
     3781 tatagacgcc ttccaccaaa acaaagttta acccgcccac cacgggaaaa ctggtgattt
     3841 cgtccatcga agcgtgatcc acaatgacga aatcgtccga aacccagtaa taggaattat
     3901 cgtagaacac gatatacttc ccgggtttca gaaagactct attccccgaa acatccactg
     3961 ttgccggtgg gtaaattata tcagttttcc accttcttct gaaagccagc ggaaattcta
     4021 taacggtttc ctcgggattg acgatctttg tgaactgggc cagaggaacc gtgaaaaaag
     4081 actcaacgac aaaattgatt atcgcaacga accatacggt cattatagta accatacaga
     4141 tcgccgctct caaagaaaga ctcatacgtc aagattggaa atgctcaaag cgtgtcgttc
     4201 tatgaacgat cttctctcct ccacctcgct acccataaga atcgtgaaaa ctctatcggc
     4261 ctcttctgca tcttccatag ttatctggac tagttttcta tcggtcgggt tcattgtcgt
     4321 ctcccatagt tgttcgggat tcatctcccc gagtcctttg aacctagagt agttcagctt
     4381 tttatcggga tacttctcga caaaggcatt cagctcctcg tcgctataaa agtaatgctt
     4441 ctgtcggttc atctctattt tgtaaagtgg cgcctgggca acgaagacct tcccggtggt
     4501 tataagcggt ttcatgtatc tgtagaaaag tgtgagaagc agcgtagtta tgtgagcccc
     4561 atctacatcg gcatcggtca tgattatgat cctgccgtac ctgagtccag caatttcgaa
     4621 gtcctcgcct atacccgtgc ccagagccac gacaatgttg cttatagtct cgctcttaag
     4681 cattttgtcg aagctggcct tttctacgtt caaaatcttg cctctgagtg gcagaatcgc
     4741 ctgggtatcc ctcgagcgag ccatttttgc cgagcccccg gccgaatcgc cctccactat
     4801 gaagatctcc gtctcttcta tgttctcgga tatacagtcg gccagttttc ccggcagcgt
     4861 cgtgttctcg agaacgttct ttctccttac catctccctg gcctttttag ctgcaagcct
     4921 tgcccgggct gcctctatgg ctttggtcag tatgagcttt aggtccttct ggttgtaatc
     4981 gaagaactct tccatcttct ctttgacgac ctttatcacg gcttcgtagg cctcttcgtt
     5041 tccgagcttg gccttcgtct ggccttcgaa ctgcggctct ttcacgaaga cactcagtat
     5101 cgcggtaagg ccttctctaa catcttcgcc ctggaggttc tgatccttct ccttcaggat
     5161 gttgtgtttt ctgccaagat cgttcatcgt ctttgtcaga accgtcttga aaccggtgag
     5221 gtgtgtaccg ccttccacag tctttatgtt gttgacaaag gtcagcgtct cctcttcgta
     5281 agcggttgtg tactgcattg cgagctggat tttaacgtcg ttgaaggagc cctcgagata
     5341 gatcgggtct ttgtggatcg tttttttgct cctgctgaga gccctgacga actcgactat
     5401 cccgccttcg aaatggtagc tcctgctctc ttctatcctt ctgtcctcga aattcaacct
     5461 cagacctccg ttgagatagg ccagctcctt gaagcgcgat tcgagaatat cgaagtcgaa
     5521 atccgtaacg gtaaagacca gcggatcggg tttgaacgtg gttacggtac cgtttatgtc
     5581 cgtctcgccg ataatatcca ccggggtaac ggcctttccc cgttcatact tctgtctata
     5641 gatctttccg tcggtcatga ccctcacttc catccactcg gaaagggcgt tcacgacgga
     5701 ggcaccaaca ccgtgaaggc ccccactgat cttgtacgaa tccttcgaga acttgcctcc
     5761 ggcatgcagg gtggtcatga cgacctccag cgcgctggta ccggtgtcgg gatgggtatc
     5821 gactggaatg cctctcccgt tgtcgcttac catgactgat ccgtcttcga gtatcgtgat
     5881 atcgattcgg tcgcaaaagc cacccatcgc ctcgtctatg ctgttatcga caatttcata
     5941 gaccagatga tgaagacctg atttcccagt agagcctatg tacattcccg gcctctttct
     6001 gacggggtcg agacctttaa gtatctttat attctgagcg ttgtatagtt cagacattca
     6061 gcacctccaa tttctatcct actctgaatc tgattctctt cacagcttca cttccaacgg
     6121 cttcatttat cttctcgata aaagcgctct ctcgcaattt caattcatgt aaccacatgc
     6181 cgtcgttgac ggccacgata agaaccccgt ccgagtaatc gacgatcgac gagtgtccgg
     6241 cgatagattc ccctactatc gtggcccagt ctctgttcaa atccaacact ctgagcttcc
     6301 tgaaaagccc gggtctgcgg gccagccctt caaatatctg ccctaaagtc tgatggttcc
     6361 acttcatcca gaccactcca gttgctgtcc tctgtcatga tataaccctc aaagttgtcc
     6421 agcaatagcc gggtcattcc atcggtgaag tcttctatca agatcttctc ttcgagtttc
     6481 cggataggta cgatcccgac actcgtgtgg gtcaggaaga gctcgtcggc agagaacatc
     6541 tcccaggaag cgacaggcct ctccataa
//
LOCUS       DAOOLY010000132         6552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14018_length_6552_cov_5.104048, whole genome shotgun sequence.
ACCESSION   DAOOLY010000132 DAOOLY010000000
VERSION     DAOOLY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14018_length_6552_cov_5.104048"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PLD15_07475"
     CDS             <1..1283
                     /locus_tag="PLD15_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB63783.1"
                     /translation="GLIRRTLTGAKREEPTNGSDIYLSIDIDLQRMVYEELKKTVEKN
                     MADGGLAILLDSKTGKVLSYAGTYNWDLGLMGIFEPGSTIKPLIYSLALTVGAINEEM
                     TFSCDGRIQPVPGLNIVIRDVEGERHGVQTFREAIINSCNVATVQVGGKIFNTLGRNE
                     MYTALEKIGFGRLSGVDLPGETPGIFPQASTWSLISPYQFPIGQGLGVNIFQMVKALN
                     VFPAGGQFIVPTFLKAIRSEDGFVNVPKKVDGILFSPELVATMLPVLESVVLNGTGTL
                     AQVDGVRIAGKTGTAQKAGPGGYNEETYYALFYGFFPVENPSYTLYIMIDTPKAGVYY
                     GGSVAAPMFASIVKKIYGIDVEKEKYEGIFGWKMPDLTGYTLIDVNEVREIYGIDKLI
                     VHGAGKVMRQFPESGHPLEDYIEVWLGVGETDGI"
     gene            1273..2199
                     /locus_tag="PLD15_07480"
     CDS             1273..2199
                     /locus_tag="PLD15_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPB63784.1"
                     /translation="MVFEMTGESLVLKEFFISARAPNCQRISSDHDEKEYKLRTVISS
                     SNMFGETAVRLDGFSRWKKEERKSVERLLKSIGSSPDVFVDGSIDPGIEKTKVSFELP
                     KPWEEERWIDHGINVASQLQMKATRDSIKRLIEKVGRDEMRILSELEKLKAVARNITA
                     SMVDRYVVKDREVEVETLVFTFLSRGSEFLSCFDYRQLPFPLFSSVLSKILIEIGTVL
                     ERKSAGNSLSWREVKSLSEATGISTSRISKIVGFNFSGSGDQNTDITAMLDRKKIAAL
                     LLCLQDLDEEFKSGDINQEIAYFALIKSSSQV"
     gene            2324..2698
                     /gene="rpsL"
                     /locus_tag="PLD15_07485"
     CDS             2324..2698
                     /gene="rpsL"
                     /locus_tag="PLD15_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPB63785.1"
                     /translation="MPTINQLIRHGRKQLKQKSASPALENNPQKRGVCVRVSTMTPKK
                     PNSALRKIARVRLSNGTEVTAYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYKIIRGTL
                     DAEGVANRKQARSRYGAKRPKK"
     gene            2713..3180
                     /gene="rpsG"
                     /locus_tag="PLD15_07490"
     CDS             2713..3180
                     /gene="rpsG"
                     /locus_tag="PLD15_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPB63786.1"
                     /translation="MRRRQSEKREVVLDPIYNDAVVTRLINKIMIGGKKSKAEMTVYK
                     ALEALSEKTKQPPMEAFRKAIGNVKPLLEVRPRRVGGATYQIPFEVPERRAVSLAIRW
                     IVSSARSKSGKPLRDKLALELIDAYNGQGNAVKKREDVHKMAEAGKAYAHFRW"
     gene            3206..5278
                     /gene="fusA"
                     /locus_tag="PLD15_07495"
     CDS             3206..5278
                     /gene="fusA"
                     /locus_tag="PLD15_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB63787.1"
                     /translation="MKERITTLEKTRNIGIMAHIDAGKTTTTERILFYTGKNYKLGSV
                     DEGTATMDWMDQEKERGITITSAATTAFWKDHRINIIDTPGHVDFTIEVERSLRVLDG
                     AIAVFDAQAGVEPQSETVWRQADKYHVPRIAFMNKMDKIGADFKAAIRTMVDKLKANP
                     IAIQLPIGSESSFDGIVDLVRMKAIKWYNQEGTEFGYEEIPESLQDEAEEAREDLIMH
                     AAEYDEGLIEIYLEGDEIPVDRLKAAIRKGTLDGKMTPVMCGTAFRNRGIQPLLDAVV
                     DYLPSPKDLPPVVAHVLDRDGEIDIFPNEEDSFVAMAFKIMVDPFVGKLTFLRVYSGS
                     LEKGSYVYNTNKNQKERISRLLFMHADKREEVDYIRAGDIVAVIGLRNTMTGETVVVD
                     EPRVVLEKIEFPEPVISIAIEPQTKDDATRLTKALVALVEEDPSLKSFVDSETGETIL
                     SGMGELHLEVIVERIKREFNVSLRVGNPQVAYRETIRSSASGEARYIRQTGGRGQYGH
                     VILRVEPIKDNSSNFLFEDKTVGGTIPREFIKPIERGVREAMDTGYLAGYPMVNIKVT
                     VLDGSYHEVDSSEIAFSIAGSMAFKEATRKASPVLLEPIMAVEINTPEEYMGDLIADL
                     NSRRAKIEGFESRGDLKIIKSHVPMSELFGYATVSRSLSQGRAIHVIQFSHYAEVPEK
                     IAEKILGK"
     gene            5311..6507
                     /gene="tuf"
                     /locus_tag="PLD15_07500"
     CDS             5311..6507
                     /gene="tuf"
                     /locus_tag="PLD15_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPB63788.1"
                     /translation="MAKEKFERSKPHLNIGTIGHIDHGKTTLTAAITKSLAFKGLADF
                     SPFDAIDKAPEEKARGITINVSHIEYSTEKRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGVMPQTREHVLLARQVNVPAMVVFINKVDAVDDEELVELVEEEVRELLSS
                     YEFPGDELPVIRGSALLALEADKPNEWTEKIYELMKACDDYFPDPIRETDKPFLMPVE
                     DIFTITGRGTVVTGRIERGAVHVGDDVEIIGLSYETKKTVCTGVEMFRKLLDEGQAGD
                     NIGALLRGVAKEDVKRGQVLAKPGSITPHKKITANVYVLKKEEGGRHSPFTKGYRPQF
                     FIKTADVTGEIADLPEGVEMVIPGDNIEMTIQLIYPVAIEKGMRFAIREGGRTVGAGV
                     VSSIIE"
BASE COUNT         1878 a         1548 c         1766 g         1360 t
ORIGIN      
        1 acggattgat cagacggact ttaacgggcg cgaagagaga agaaccgacc aacgggagcg
       61 acatttatct ctccatagat atagatctcc agagaatggt ttacgaagag ctcaaaaaaa
      121 ccgtcgaaaa aaatatggcc gatggagggc ttgccatact tctcgatagc aagaccggca
      181 aagtactctc ctacgccgga acatacaact gggatctcgg cttgatggga attttcgaac
      241 cgggatcgac cattaaacct ctgatctatt cgcttgccct cacagtggga gccatcaacg
      301 aagagatgac tttcagctgc gacgggagga tacaaccggt ccctggactg aacatagtca
      361 tacgcgacgt cgaaggtgaa agacacggtg tccagacctt cagggaggcg attataaact
      421 cctgcaacgt ggcgaccgtg caggtcggtg gaaagatttt caacactctt ggaaggaatg
      481 aaatgtacac cgcgcttgag aaaataggct ttggaaggct atcgggagtg gatcttcccg
      541 gagagacgcc ggggattttt ccgcaagcct ctacctggtc gctcatatcg ccttaccagt
      601 ttccgatcgg tcagggtctg ggtgtgaaca tctttcagat ggtcaaggct ctcaatgtct
      661 tcccggcggg cggtcagttc atcgttccca cttttttgaa agccataagg agcgaggatg
      721 gattcgtcaa cgttccgaag aaagtcgatg gaattctctt ctccccggaa ctggttgcaa
      781 cgatgctccc cgttctcgag agcgtcgtcc tgaacggtac gggaacgctc gcgcaggtcg
      841 atggggttag aatcgcagga aagaccggaa cggcccaaaa agccgggccg ggcgggtaca
      901 acgaggaaac ttactacgcg ctcttttatg gattcttccc ggtggaaaat ccttcataca
      961 cgctgtacat catgatcgac acaccgaagg ccggtgtcta ttacggtggc tctgtggccg
     1021 cccccatgtt cgcttcgata gtcaaaaaga tatacggaat agatgttgaa aaagagaagt
     1081 acgaaggaat attcggctgg aaaatgccgg atctaaccgg ctatacactg attgacgtga
     1141 acgaggtaag ggaaatctac ggaatcgaca agctcatcgt gcacggggct gggaaggtca
     1201 tgaggcagtt ccccgagtca gggcaccctc tggaagacta catagaagta tggctgggag
     1261 ttggagagac cgatggtatt tgagatgacc ggcgagagtc tggtgctgaa agagtttttc
     1321 atatcggcca gagctccgaa ctgccagagg atatcctccg atcacgacga aaaagaatac
     1381 aaactcagaa cggttatctc ctcttcgaat atgttcggcg aaacggccgt caggctcgac
     1441 ggtttctcca gatggaaaaa ggaagagagg aaatcggtag agagacttct caaatcgatc
     1501 ggttcatcgc ccgatgtctt tgtggatggt tccatagatc cgggcatcga aaagacaaag
     1561 gtatctttcg agctccccaa accgtgggaa gaggaacgat ggatagatca cgggatcaac
     1621 gtcgcctcgc agttacagat gaaggctact cgcgattcga tcaagagatt gatcgaaaaa
     1681 gtcggaagag atgagatgag gatcctgagc gaactcgaaa agctcaaggc cgttgcccga
     1741 aatataaccg cctcgatggt cgacaggtac gtggtgaagg atagggaggt cgaagtcgag
     1801 actctggtct tcactttttt gagccgcggt tcggagttcc tgtcgtgttt tgactacagg
     1861 caattaccct tcccgctgtt ctcctccgta ctctcgaaga tcctgatcga gataggcacg
     1921 gtgctcgaac gtaaaagcgc ggggaactcg ctctcgtgga gggaagtcaa atcgctctcc
     1981 gaagcgaccg gcatctcgac atcgagaatc tcgaagatcg taggattcaa cttctctggc
     2041 agcggcgacc agaacaccga tattaccgct atgctcgacc ggaaaaagat cgccgctctc
     2101 ctgctgtgcc tccaggatct ggacgaggaa ttcaaaagtg gcgatataaa ccaggagatc
     2161 gcctacttcg ctcttataaa gtcttcttcg caagtgtaag taaaatgtct gtaaatttct
     2221 ttttttcaac atcataagga cgccgttcta atttgacagt tccaaaagtc cattgtacaa
     2281 ttgctttcgg caaaatgctt agaaggaggc gaatcgaaac cagatgccca caataaatca
     2341 attgataaga cacggaagaa aacagctgaa acagaaatct gcttcaccag ctctagaaaa
     2401 caatcctcag aagcgtggtg tttgtgtaag ggtctcgact atgactccca agaagccaaa
     2461 ctctgctttg agaaagatcg caagggttag actttcgaac ggaaccgaag taacggctta
     2521 cattccagga gaaggccaca accttcagga gcactccgtt gtactggtta ggggcggtag
     2581 agtcaaagac cttcccggtg ttcgctacaa gataatcaga ggcactctcg atgccgaagg
     2641 agtcgccaac aggaagcagg ccagaagccg ctacggagca aagagaccca agaaatgagg
     2701 aggcaagtct agatgagaag acgacagtcc gaaaagcgcg aagtagtact ggatccaata
     2761 tacaacgatg cagtggtcac gagactgata aacaaaataa tgataggcgg aaagaagagc
     2821 aaggcggaga tgaccgtcta caaggcgctc gaagcccttt cggaaaagac caagcagccg
     2881 ccgatggaag ctttcaggaa ggctatcggc aacgtcaagc ctcttctgga agttcgaccg
     2941 cgaagagtcg gcggtgcgac ctatcagatt ccattcgaag taccggaaag aagagcggtt
     3001 tctcttgcga tcagatggat agtctcctcc gctcgctcga aatcaggtaa gcctctcagg
     3061 gacaagctcg ctctcgagtt gatagacgcg tacaacggcc agggaaacgc cgttaagaag
     3121 agagaagatg tccataaaat ggcggaggct ggaaaggcat acgctcactt cagatggtaa
     3181 gcgtccttcc gcgggaggga agatagtgaa agagagaatt actactttag aaaaaacccg
     3241 aaatattggc ataatggccc atatagacgc cggtaaaact acgactaccg agcggattct
     3301 tttctatacc ggaaagaatt acaagctcgg aagcgtggac gaaggtaccg cgactatgga
     3361 ctggatggac caggagaagg aaagaggaat cactatcact tctgccgcta ctacagcatt
     3421 ctggaaggac cacaggatta acattatcga tacacccgga cacgttgact ttacaataga
     3481 agtcgagaga tcgctaagag tgttggatgg agcgattgcc gttttcgatg cccaggccgg
     3541 agtggagcct caatcggaga cggtctggag gcaggccgat aagtaccacg tgccccgcat
     3601 agccttcatg aacaaaatgg ataaaatcgg agccgatttc aaagccgcga ttagaaccat
     3661 ggtggacaaa ttgaaggcca atcctatcgc gattcaactt ccaatagggt ccgagtccag
     3721 ctttgacggg atagtggatc tggtaagaat gaaagcgata aagtggtaca accaggaagg
     3781 tacggagttc ggctacgaag agatccccga gtcccttcaa gacgaagcgg aagaggcccg
     3841 tgaagatctg ataatgcacg cggccgaata cgacgaaggg ctaatcgaga tttacctcga
     3901 aggggacgaa attcctgtcg acaggttgaa ggccgccata agaaagggaa ctctcgatgg
     3961 caaaatgaca ccggtcatgt gcggaactgc tttcaggaac aggggtattc agccgcttct
     4021 ggacgccgtt gtagactatc tgccttcgcc gaaggatctg ccgccggttg ttgcgcacgt
     4081 gctcgaccgc gacggggaga tagatatctt tcccaacgag gaggactcct ttgtagccat
     4141 ggctttcaag ataatggtcg atccgttcgt gggaaagcta acttttttga gagtctattc
     4201 cggttccctt gaaaagggat cgtatgttta caacaccaac aagaatcaaa aagagaggat
     4261 ttccagactt ctgttcatgc acgccgacaa aagagaagaa gtcgattata taagggctgg
     4321 agatatagtt gctgtgatcg gtttgagaaa caccatgacc ggagagacgg tagtcgtcga
     4381 tgaaccgcgg gtcgtgctgg aaaagatcga gttccccgaa ccggtaatat ccatcgccat
     4441 cgaaccccaa acgaaggacg acgcgacaag attgactaaa gcgctggtcg cactcgtgga
     4501 ggaagatcct tcgttgaaga gtttcgttga cagcgaaacg ggagaaacga tactttcggg
     4561 tatgggcgag ttgcacctgg aagtcatagt cgagaggata aagagagagt tcaacgtttc
     4621 ccttcgtgtt ggaaaccccc aggtggccta tcgcgaaacg ataaggtcct ccgcctcagg
     4681 agaagccagg tacataagac agaccggcgg taggggccag tacggtcacg taattctcag
     4741 agtcgaaccg ataaaagaca acagctcgaa cttccttttc gaagacaaga ccgtgggtgg
     4801 aaccataccc agagagttca taaaacctat agagcgcggc gtgagagagg ctatggatac
     4861 tggatatctt gccggctatc caatggttaa tataaaggtc acggtacttg acggctccta
     4921 tcacgaagtg gattcttccg agatcgcttt cagcatagcc ggttcaatgg cctttaagga
     4981 agctaccaga aaggccagcc cggttctgct cgagccaata atggccgtag aaataaacac
     5041 ccccgaagaa tatatgggtg atctcatagc cgacctgaac tcgagacgcg ccaagatcga
     5101 aggatttgaa tctagaggcg atctgaagat catcaaatct cacgtaccca tgtcggaact
     5161 attcggctat gcgacggtca gtagatctct ttcgcaggga agggccattc acgtaatcca
     5221 gttctcgcat tacgcggaag taccggagaa aatagccgag aaaatacttg gaaaataaag
     5281 atcccaaaaa gtttttggag gagggaaacc atggctaagg aaaaattcga acgttctaaa
     5341 ccccatctta acatcggtac catcgggcat atcgaccacg gcaagacgac acttactgcg
     5401 gcaataacga agagtcttgc tttcaaaggc ctcgcagact tctcgccgtt cgatgcgatc
     5461 gataaggcac ccgaagagaa ggcaagagga atcacgatca acgtttcgca catcgaatat
     5521 tcgaccgaga agagacatta cgcacatatc gactgccccg gacacgcaga ctacataaag
     5581 aacatgatca cgggtgcggc ccagatggac ggagccattc tcgttgtcgc cgccactgac
     5641 ggcgttatgc cccagaccag ggaacacgtt cttctcgcca gacaggtcaa cgttcccgcg
     5701 atggttgtat ttataaacaa agtcgacgcc gtcgacgatg aagagctcgt cgaactcgta
     5761 gaagaagaag tcagagagct tctctcgtct tacgaattcc cgggcgacga acttccggta
     5821 atcaggggtt ctgcccttct cgctctggaa gcagacaaac cgaatgagtg gacggaaaag
     5881 atatacgaac tgatgaaggc ctgcgacgat tacttcccgg atccaataag agaaaccgac
     5941 aagcccttcc tgatgccagt tgaagacatc ttcaccatca ccggaagagg aacggtcgtc
     6001 accggtagaa tcgaacgcgg tgcggtccac gtgggagacg acgtcgaaat catcgggctc
     6061 tcttacgaaa ccaagaagac agtctgtacc ggagtagaaa tgttcagaaa gctactcgac
     6121 gaaggacagg ccggagacaa catcggagct cttctcagag gagttgcgaa ggaagatgtc
     6181 aaaagaggtc aggtccttgc caagcccgga tcgatcactc ctcacaagaa gattactgcc
     6241 aacgtttacg tcttgaagaa ggaagaaggc ggacgccatt ctccattcac gaagggttac
     6301 agaccgcagt tcttcattaa gacggccgac gttaccggag agatcgcaga tcttccagag
     6361 ggtgtagaga tggttattcc cggcgacaac atcgaaatga cgattcaact gatctacccg
     6421 gtcgccatcg aaaagggtat gagattcgct atccgcgagg gtggcagaac cgtgggcgcc
     6481 ggagtcgtca gctccataat cgagtgattt aagggtcttt ccaggagaag ggagatttct
     6541 ctcccttccc tg
//
LOCUS       DAOOLY010000133         6518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14125_length_6518_cov_5.000928, whole genome shotgun sequence.
ACCESSION   DAOOLY010000133 DAOOLY010000000
VERSION     DAOOLY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14125_length_6518_cov_5.000928"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1891)
                     /locus_tag="PLD15_07505"
     CDS             complement(<1..1891)
                     /locus_tag="PLD15_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPB63789.1"
                     /translation="MIRLVSFATLIAVVVVLFLGIGGLYFEPSPGSFLSDDDPELMAF
                     SRVAREFGDTGTVMIVLNSSGLSLEKLKEVTQKIDGLDWVNAVASVFDAVKLGKLNLL
                     TLQIPRTEYVSQSDGKLVLDMDILDDPFYRDLIISSDGDYYGILITVASGNEFKSGSN
                     ILSLKATLKELGIKEYRLIGESVANSTTFRSIMDLTFVYPPFIFLAIFTVYIVKFRRI
                     SLALLTLVPPAGAAVLVVGIMGLINTQINSLTVMIPSFIVIIGSAYGMHFLSRFQENC
                     ATQENPIRRTIHEERVPILFSALTTMAGFSSYILLDMRAFRDMGMLVCVGIFSSAVFT
                     LSILPGLVSPQRCAKVSLTSAPVQIPPRIRKTLLWVVVVMCAASPVLIMTIPMSIDQY
                     AFFKENSEIRQNAEKMKEAFGWLTNYTLMITPEGGGKLAITGEQASQLVDFQKELEAL
                     EDISKVLSPVDISRQTNIPLPLLIRALDNTGVLGSATSLMISQRALRVNLFSPASDSL
                     TAEKLKSSVRELIAKYPLLNEKFDFTLAGTTLIWKSVNSSVVSNQIQSLVVSFGLILL
                     LLFTIFKSVKSTLIATIPIALTTLFNFIFMALFRISLSISTALISGMLMGLVIDYAIH
                     FTIWLR"
     gene            2083..2838
                     /locus_tag="PLD15_07510"
     CDS             2083..2838
                     /locus_tag="PLD15_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63790.1"
                     /translation="MIICRKLTKTFDVTTVVDGLDLQVRAGEIYGFLGPNGAGKTTTI
                     RMLTGTLRPTSGEVEILGMNFAANELQIKSRIGVVPDEPRIYSYFTGMEFLEFIMDIF
                     PAKKGEAMKRVGELCEAFGVDYLGKMISEMSHGMKQKIMVISVLMRRPEVIFLDEPTV
                     GLDARSAKILKMLLEKYKNEGSTIFMTTHVLEIAEKMCDRIGIINKGRLISQGTMEEM
                     RKSMGAGERESLEDIFLQLTGENEDIQDIVNAL"
     gene            2843..4354
                     /locus_tag="PLD15_07515"
     CDS             2843..4354
                     /locus_tag="PLD15_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63791.1"
                     /translation="MSGLNDLKLLFKYKLFIFPNKNGTRGGFSPQLMVFLIFTCMIGI
                     FLWNVFGSIRGVEIDGTPIATVMAGFFLTVSGLFFIMSFSATTSYLFIRNSEIDMLLV
                     LPVRRTSIAIYQIAISTVYQGLTLSVFLGIALSYHLRADSRPIVGILALLALLIDTVL
                     LASIISVAFGRFMSRAAARKLMFVVQIISGFMFFIIVQLVPRNPDNIPVYLQKLTGAW
                     EILANPVNLFTWSVRASGEPLFLLLSVVMIPVFGAIFYRIAGSLKFEPLSYSRSRERR
                     VVFSEKRGSALIRRELKIYRRYEQLIYYLFYPVIFGLIFGLISEDLTTSIFTMVLIST
                     IFTTIQSAFLMGREYPFIETTKALPLSLERIVFIKTILPVLLGILLFTGVLLISILVK
                     NTTFLYLAVVPIVFVLYVTSSLLGIKGVLESPPDRMDNPNAFMRTKFVLLNQIICMGL
                     SFGSIMPITMIMGGAPGSPGIWIAVLISLSSTAVAAVLSVFTRRRVLAKMKKV"
     gene            4401..5084
                     /gene="elbB"
                     /locus_tag="PLD15_07520"
     CDS             4401..5084
                     /gene="elbB"
                     /locus_tag="PLD15_07520"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPB63792.1"
                     /translation="MCRAGVILSGCGGKDGTDIAEAVMLFLALDRAGVETICMSIDKD
                     QFEVVNHLEGSTQTSGQKRNMLIESARLACGGIVNLDGVENVDFDILVIPGGKGVLKN
                     LSTFMSEELEFRVNSVVQRIIVNTHLSGKPVGAMCGAVVLLAKSLENFVESLKITATG
                     QSEEGRIAIERAGFSHHDCQEGEVLIDDENLIVTSPALSPDVDLEVVRSTAESMVKAL
                     LTLITDKAL"
     gene            complement(5098..6039)
                     /locus_tag="PLD15_07525"
     CDS             complement(5098..6039)
                     /locus_tag="PLD15_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPB63793.1"
                     /translation="MALSSLLLKLRKLLPLLGVALFLVSVAVLIRELGRYTISDIADK
                     VGAIPLSRFLTAVMLTFLAYANLTLYDLISSLHLGVKKGYGRTALVSFIAYTFSKNIG
                     FTFLSGTSVRFRMYGKWKVGSGKILGIIVLNYLTFWLGFFALSGVVFTLWYPRMQTVI
                     RMPFSSLQIFGAIAMAIYCLYLFMVIIRKKPFRFRNFRFSLPGKAFTFSQTAISTVDW
                     LLSAGVLYFLLPPAQYVPILAVFLLSQFAGLSSQVPGGLGIFEAMTLVILSPLIEADV
                     LIAALLLYRVIFYLIPFLVALILFGLEEFLWSRLMRQ"
     gene            complement(6040..>6518)
                     /locus_tag="PLD15_07530"
     CDS             complement(6040..>6518)
                     /locus_tag="PLD15_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HPB63794.1"
                     /translation="VIGTAPAKESISCGTPGFHGGNLDTNDICAGSKVFLPVFVEGAN
                     LALGDVHARMGNGEICGTGIEIRATVRISVKLDRSSIDGPMVENDNAFYMLYSAKTFD
                     DATRGAVERAVRFIADRKSIPQPVAYMLASVTCDLTVSQIVNPLVTVRVKIPKEIL"
BASE COUNT         1663 a         1580 c         1701 g         1574 t
ORIGIN      
        1 ttcggagcca tatggtgaag tgtatcgcat aatcgatcac caaacccatg agcatcccgg
       61 atatcagggc cgttgaaatg ctcaggctta ttctgaacag ggccatgaag atgaagttga
      121 aaagcgtggt cagcgcgata ggtatagtgg caatcaaagt ggattttacg cttttaaaga
      181 tcgtgaaaag caaaagcagt atcagcccga aagagaccac caggctctgt atctggttgg
      241 acaccaccga cgagttaacg ctcttccata ttagcgtcgt accggcgagg gtgaaatcga
      301 atttttcgtt gagaagggga tacttcgcga taagctctct cacagagctc ttcaatttct
      361 ccgcggtgag gctgtcgctg gccggagaga agaggtttac cctcagggct ctttgactta
      421 tcataagcga ggtcgcgctg ccaagaacgc cggtgttgtc cagagccctt attagcaaag
      481 gcagaggaat gttggtctgg cgggagatat cgaccgggct cagcactttg gatatatcct
      541 ccagggcttc cagttctttc tggaaatcga ccagttgact cgcctgctct ccggttatgg
      601 ccagttttcc gccgccttcg ggtgtgatca taagcgtgta gttggttaac cagccaaagg
      661 cctctttcat cttctctgcg ttctggcgga tctcagaatt ctctttgaag aaggcatact
      721 ggtctatgct catggggata gtcattatca gtaccgggga ggccgcgcac atgactacaa
      781 ccacccatag caaagtcttt ctgatcctcg gcggaatctg caccggtgcc gacgtgagag
      841 agactttggc gcacctttgc ggcgatacca gtccgggaag tatgctcaag gtgaaaacgg
      901 ccgaggagaa gatacctacg cagaccagca tccccatatc gcggaacgcc cgcatatcca
      961 ggaggatgta tgaagagaag ccggccatcg ttgtgagggc agaaaaaagt atcggcaccc
     1021 tctcttcgtg aatggtccgc cttataggat tttcttgtgt ggcgcagttc tcctggaatc
     1081 tcgatagaaa gtgcatgccg taggcgcttc cgattatcac aatgaaagac ggtatcatca
     1141 cggtgaggct gttgatctgc gtgttgatca atcccattat accgaccacc agaacggcgg
     1201 cgccggcagg gggaacgagc gtgaggaggg caagcgagat ccttctgaat ttgactatgt
     1261 aaactgtgaa gatcgctaga aagatgaacg gtggatagac gaaggtcagg tccattatcg
     1321 acctgaaggt cgtcgaattg gctacgctct cgcctatcaa tctgtattct tttataccca
     1381 gttctttcaa agtagctttc agcgagagta tattcgaacc gctcttgaat tcatttccgc
     1441 tcgcaacggt tatcagaatg ccgtaatagt cgccgtctga agatatgatc aggtctctgt
     1501 agaagggatc atctagaata tccatatcca gaaccagttt tccgtctgac tgggagacat
     1561 attccgttct ggggatctgc aacgtcagga ggtttagctt tcccagcttg acggcatcga
     1621 acacggaggc tacggcgttt acccagtcga gtccgtcgat cttttgagtg acctctttca
     1681 acttctcgag cgagagaccg gaggagttca gcactatcat caccgttccc gtgtctccga
     1741 actcccgggc gacacggcta aacgccatca gctcgggatc atcgtcgcta agaaacgacc
     1801 ccggagatgg ttcgaaatag agaccgccga taccaagaaa cagaactacc acgactgcaa
     1861 tcaacgtggc gaaggagacc agccttatca ttcaaacacc tcacagactc tggatattat
     1921 atcatctatg gtagagttga aaggaggtgt tcgagaggat cccgtttccg tatcgacctc
     1981 cccggagtgg aggacaacga cggccggacg acgtagaata tacattggtc ggagagtgtg
     2041 atgttattta aatgaattac ggaacgatgg agggaaacga ctgtgattat ctgcaggaaa
     2101 ttaacgaaaa ctttcgacgt gacaaccgtt gtcgatggcc tcgatctcca ggtgagagcc
     2161 ggggagatat atgggtttct gggtcccaac ggggccggga aaacgactac gatcagaatg
     2221 ttgaccggaa ctctgagacc gacctccgga gaggtggaga tactcggcat gaacttcgcc
     2281 gcgaacgaac tccagataaa gagcaggata ggggtcgtcc ccgacgagcc caggatctac
     2341 tcgtacttca ccggaatgga gtttctcgaa ttcataatgg atatttttcc agcgaaaaag
     2401 ggagaggcca tgaagagagt cggcgaactg tgcgaggctt ttggagtcga ttatcttggg
     2461 aagatgattt ccgagatgtc tcacggcatg aagcagaaga taatggttat ttccgtcctc
     2521 atgagaagac cggaagtcat atttctcgat gaaccgaccg tcggtctcga cgccaggagc
     2581 gctaaaatat tgaaaatgct tctggaaaaa tacaaaaacg agggctccac gatcttcatg
     2641 accacgcatg ttctcgagat cgccgaaaaa atgtgcgaca gaatcgggat catcaacaag
     2701 ggaaggctca tatctcaggg aactatggaa gagatgcgca aatccatggg cgcaggcgag
     2761 agagaaagcc tcgaagacat cttccttcag ctaaccggag agaacgaaga tatacaggac
     2821 atagtgaacg ccttatgagg gagtgagtgg cttgaacgat cttaaacttc ttttcaagta
     2881 caagctgttc atcttcccga acaagaacgg aaccagaggc ggcttttcac ctcaactcat
     2941 ggtttttctc attttcacat gcatgatcgg tatctttctg tggaacgtat ttggctcgat
     3001 acggggtgtc gaaatcgatg gcactccgat cgcaactgtt atggccggct tttttctcac
     3061 cgtttccggt ctgttcttta taatgtcttt ctccgcaacc acttcgtatc tcttcataag
     3121 aaacagcgag atcgatatgc tgctggtttt gcccgtcagg aggacaagca tagctatcta
     3181 ccagatagca atctccacgg tctatcaggg gttgacgctt tcagtctttc tcgggatcgc
     3241 tctttcgtac cacctgaggg ccgattccag accaatcgtc ggaatccttg cgctcctggc
     3301 tctgctaatc gatacggtcc ttctagcctc gatcatttcc gtcgccttcg gcagattcat
     3361 gtccagagcc gcggcgagga agctgatgtt cgtggtgcag ataatcagtg gattcatgtt
     3421 ttttataata gtacagctcg tcccaaggaa ccccgacaat ataccggtgt atctccagaa
     3481 actgactggc gcctgggaga tactggccaa tcccgtgaac ctcttcacct ggagcgtcag
     3541 agcatcgggc gaaccactct ttcttcttct cagcgttgtg atgatcccgg ttttcggcgc
     3601 catcttctac aggatagccg gctcgctcaa attcgagccc ttgtcctatt cccggagtag
     3661 ggaaagacgt gtcgtgttca gcgagaaacg cggcagcgcg ctgatccgta gggaattaaa
     3721 gatatacagg cgttacgaac agctcatcta ctatcttttt tatccggtga tcttcggttt
     3781 gatcttcggc ttgatatcgg aggacctcac gacttccatc ttcacgatgg ttctcatcag
     3841 cacgattttc accacgatcc agtcggcctt tctcatggga cgggagtatc ccttcatcga
     3901 aactaccaag gctctcccgc tttcgctgga aaggatagtg ttcatcaaga cgatcctccc
     3961 cgttttactc ggcatacttc ttttcacggg agttctcctt ataagcattc tcgtgaagaa
     4021 caccacgttt ttgtatcttg cagttgttcc gatcgtcttc gtactatacg tgacttcctc
     4081 tttactcggt ataaagggag ttctcgagag tcctcccgat cgtatggata acccgaatgc
     4141 cttcatgcga actaagtttg tgttacttaa ccagataatc tgtatgggtc tgagctttgg
     4201 atctataatg ccgataacaa tgatcatggg cggtgcacca ggcagtcctg gaatatggat
     4261 cgcggtgctg atatcgcttt cttcgacggc cgtggcggcc gtgctgtcgg ttttcacacg
     4321 caggagagtt ctggcaaaga tgaagaaagt atgaaaccgg ttatttgaga tccctatccg
     4381 aaatggacag gaggtgctat atgtgcagag cgggagttat actctcgggg tgcggcggga
     4441 aagatggaac cgatatagcc gaggcggtga tgctgtttct tgcactcgat agagccgggg
     4501 tcgagactat atgcatgtct atagacaagg atcagttcga agttgtaaac catctggaag
     4561 gcagtacaca gacgagcggg cagaagagga acatgctcat cgagtcggcg cggctggcct
     4621 gcggaggtat tgtcaacctg gacggcgtcg aaaacgttga tttcgatata ctggtaattc
     4681 caggtggtaa gggagtgttg aagaatctat cgacctttat gagcgaagaa ctggaatttc
     4741 gcgtcaatag cgttgttcag aggataatag tgaatactca tctatctggc aaacctgtcg
     4801 gggccatgtg cggagcggtg gttctgctgg ccaaatcgct ggagaacttt gttgaaagtc
     4861 tgaagataac cgccaccggt caatcggagg agggcaggat cgcgatagag agggcaggat
     4921 ttagccacca cgattgccag gaaggagaag tccttataga cgacgagaat ttgatagtta
     4981 catcccccgc cctgtcccca gatgtcgacc tcgaggttgt gagatcgacg gccgaatcga
     5041 tggtgaaggc actcctgact cttataacag acaaagcgct ttagtctaac ccttcattca
     5101 ttgacgcatg aggcggctcc agagaaactc ttccagacca aacagtatca gggcgaccag
     5161 aaaggggatc agatagaaaa tcactctgta cagtaaaaga gcggcgatca gcacatcggc
     5221 ctctattaaa ggcgaaagaa taaccagggt catagcttcg aagatgccta gtcctccggg
     5281 tacctggctg gaaagaccgg cgaactgtga aagcagaaaa acagccagga tcggtacata
     5341 ttgggctgga gggaggagaa agtaaaggac ccctgccgac agaagccagt ccaccgtgga
     5401 aatcgccgtc tgtgagaatg tgaaagcctt gcccggtaga gagaatctga agtttctgaa
     5461 tctgaacggc ttttttctga taatcaccat aaaaagataa agacagtaaa tcgccatcgc
     5521 gatagctcca aagatctgaa gcgagctgaa aggcatccgt atcaccgtct gcattcgcgg
     5581 ataccagaga gtgaagacaa ctccagagag cgcgaagaaa cctagccaga aagtcaggta
     5641 attcaatacg attattccga ggatcttccc ggaaccaact ttccactttc cgtacattct
     5701 gaatcttacg ctggtgccag acagaaaggt aaaaccgatg ttcttgctga aggtataagc
     5761 gatgaaggaa accagagccg tcctgccgta accctttttc acgcccagat gaagcgaaga
     5821 gatcaaatcg tacagtgtca ggttcgcata agcgaggaat gtcaacatta cggccgtcag
     5881 aaacctggac agggggatgg cgccgacctt gtcggctatg tcgcttatgg tgtatctacc
     5941 cagttctctt atcagaaccg ccacggaaac caagaacagg gccaccccaa gcaggggaag
     6001 cagctttctc aacttaagca gtagagagct cagtgccatt caaagtatct cttttggtat
     6061 ctttacgcga acagtaacta gcgggttgac gatctggcta accgtgagat cgcacgttac
     6121 gctggccagc atgtacgcca ccggctgtgg aatcgatttc ctgtcggcta tgaagcggac
     6181 ggctctttca actgctcccc tggttgcatc gtcgaaggtc tttgcactgt aaagcatata
     6241 gaaagcgtta tcgttttcaa ccatcgggcc gtcgatagaa cttctatcca gcttgacgct
     6301 tatcctgacg gtcgctctta tctctattcc cgttccacag atctctccgt tgcccatcct
     6361 ggcgtgcaca tcgccgagcg cgaggttggc cccttccaca aagaccggga gaaagacctt
     6421 cgatccggcg catatatcgt tggtatcgag attgcctccg tgaaagccgg gcgttccgca
     6481 agatatcgat tcctttgcgg gcgctgtccc tatgacgc
//
LOCUS       DAOOLY010000134         6493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14191_length_6493_cov_4.811587, whole genome shotgun sequence.
ACCESSION   DAOOLY010000134 DAOOLY010000000
VERSION     DAOOLY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14191_length_6493_cov_4.811587"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..159
                     /locus_tag="PLD15_07535"
     CDS             16..159
                     /locus_tag="PLD15_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPB63795.1"
                     /translation="MRGRYGVSVRKNLIHASDSLESALREIELFFEEGEIMDYTLTLE
                     GEL"
     gene            159..1334
                     /locus_tag="PLD15_07540"
     CDS             159..1334
                     /locus_tag="PLD15_07540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPB63796.1"
                     /translation="MIVARLKKGKERMILNGYPWVFRDELLSIDGPQRLGEVNVFSYD
                     YGFLGRGFYNPESNRAVMVLTNRDEDIGKVLFKRRIMRALRKREALYELPYYRLIHGE
                     GDKLPGLVVDRYGEILVVQFRNRIVQSLKETIIETLVEETSPAGIYERSDFEMGVEDK
                     IDRNTGLLYGEVPKRIEVIENGLKFLVDIEKSQKTGFFFDQRESRAFCSSLVKKLSLK
                     KGLDLFSFTGAFGINMAAAGAEVICVDKSVEDLKLAKLNSELNGLDKCVQFAQSDVID
                     FIGRFEKDDAFDIVVLDPPSLVKHRKELSTGVGLFRDMIKGLLPLVRDRGVLGICTCA
                     YNIGLEHLVEAVRRSTENSGITFSHLAVTLQSPDHPWLMQVPESLYLKCLWGTIEKE"
     gene            1340..2200
                     /locus_tag="PLD15_07545"
     CDS             1340..2200
                     /locus_tag="PLD15_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63797.1"
                     /translation="MPGFWTHIIFGKDVLQEVGLDPDSERKGEFNLGCLFTDIGKYCE
                     KNSPEYLLWKLSHTTGCDAFAGVLCRELEGVSRFYVMGVFCHFFLDLSVSQYIMARAG
                     TGYRSGQLEIMIDRTILKERLTADILKLRPLAELPPSIDEELERTIQRTAGEVYNIDS
                     PSITRAVDSMKDIFDDYYGRSRLFLFFKALFLKGESIESAFFRDTHDDPMNFGMRPWF
                     HSIAGWESRLTFRDLYGKAVSDAVRFLHRYLDGEEDWSLPAMSLMTGLPLMEQEDNKN
                     VEGRETTARH"
     gene            2222..3349
                     /gene="glgD"
                     /locus_tag="PLD15_07550"
     CDS             2222..3349
                     /gene="glgD"
                     /locus_tag="PLD15_07550"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HPB63798.1"
                     /translation="MKVLGIILAGGRGEYLGPLTQVRTSAALPVFGKYRSIDFTLSNM
                     VNAGISKVGIITQYSPRSLMDHIGSGKEWDLDRKQGGLFILQPYYSPYNPSMGYKGTA
                     DAIFQNINILRRGNEDYVMIGSGDHIFKADLSEIFRYHLETGSDITALTASNIENYNF
                     GNVSLLKCKDGRVVEWIEREGPLEDLGGSNCVALGVYFMNKFLLRELLYSTVPNGENE
                     LVKGVIIPNLGSLNVREYRYNGYWRDIKQSKSCYYRTNLDILNPEIRRELFYEHGRVF
                     TKLKDLPPPKITGTAFISNSVIADGCVVGGKLEDSVLFRDTRVLAGATVKGSVLLEGC
                     IVEEGAYVENVILDKYCTVRSGKSFVGEQSDPTVIEKHGVI"
     gene            3366..4634
                     /locus_tag="PLD15_07555"
     CDS             3366..4634
                     /locus_tag="PLD15_07555"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPB63799.1"
                     /translation="MAKKVVALILAGGQGTRLGILTDEIAKPAVPFGGKYRIIDFALS
                     NCVNSGIYTVGVLTQYRPHVLSRHIGIGRPWDLDRKDGGVVILPPYVAQDDSNWYKGT
                     ANAVYQNIDYIDAYSPEIVVVLSGDHIYSMDYNEMIDYHLSKGADGTVACMRVPLSEA
                     HRFGMMVTDFENRIIDFQEKPEKPRSDLASLGIYVFNWSFLKERLLEDEQTPESSNDF
                     GKNILPETVDRDLGKLFAFVFEGYWRDVGTLQALWESNIELTRPIPPLNLHDPAWRFY
                     TQTEEYLPAYVGRSSRVRNSLINEGAEIYGRIDSSVIFQGVQIGEGSVVRNSVIMTNT
                     RIGKGVIIDKAIIGENSVINDDCVLGYGEEVPHETHPGIYDSGIVVVGYDAVIPAGTK
                     IGKNCAIANHVREGDFSGDVQGGKTIAAKE"
     gene            4597..4947
                     /locus_tag="PLD15_07560"
     CDS             4597..4947
                     /locus_tag="PLD15_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63800.1"
                     /translation="MYRAERRSLRRNDGSLSLGLLIALLFVAAAMTSMMTPLSLATSA
                     FRECLWEFRLAITLKSIYLSDFEEVEMNGLKIEKSSRSVFSDKTEIEIIHYHITEGSN
                     SFDFEKVGKITNIS"
     gene            4961..6418
                     /locus_tag="PLD15_07565"
     CDS             4961..6418
                     /locus_tag="PLD15_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB63801.1"
                     /translation="MKKFLLIALIVTLSIFTLAQRLTILHINDTHGHIWPEGEFGGLA
                     AIATLVNQARMSNPNTIFLHAGDINTGVPESDLQDAAPDIVALNLMKLDAMTLGNHEF
                     DNDATVLAKQMDLATFPFLSANIYKDGKPAFTQYTIKEVGGIKVAIVGFTAQETEILE
                     YLYAKDYEWKEVVEVAKELIPQLEAQADIIVALTHMGSNPVLVGPNSWELAAAVDGID
                     VIVDGHSHTFYERPEVINGTLIVSAGEWSKYLGKLDLEVINGTITFVSFRPIKVESSI
                     APDFAVATVLDYFKKAGGEALNVVVGETTIKLEGDRNVVRAQDTNLGYLICDAMVWKS
                     GADIAISNSGGIRASIAAGPITYRDILTVLPFGNSLYVIEISGADFMKVLEFTATIAA
                     GQGAMPQVSGVSYTIENGTVKDVLVNGEPVVMDKIYKVATNNYLASGGDGYTALANHP
                     GYDTGFVLADVVVEYVGQISPITSYQESGRINRVK"
BASE COUNT         1727 a         1493 c         1778 g         1495 t
ORIGIN      
        1 gccgacgcgg gaagcataag gggcaggtac ggagtatcgg tccgaaagaa ccttattcac
       61 gcttctgaca gcctcgagag cgctttgaga gagatagagc tgtttttcga agaaggagaa
      121 ataatggatt acaccctgac cctcgaggga gagctttgat gatcgtcgct cgcttgaaaa
      181 agggaaagga aagaatgata ctcaacggtt acccgtgggt attcagggat gaactgcttt
      241 caattgacgg tccgcagcgg ctaggagagg tgaatgtctt ctcgtacgat tacggctttc
      301 tgggaagagg cttctacaac cccgagtcga acagagccgt gatggtcctc acgaacagag
      361 atgaggatat aggcaaagta ttgttcaaaa gacggataat gagggcactg agaaagagag
      421 aggccctgta cgaactccct tactacagat tgattcatgg agagggagac aaactgcccg
      481 gcctggttgt tgacaggtac ggcgaaatcc tcgtcgttca attcaggaac cggatcgtgc
      541 agagtcttaa agaaacaata atcgaaacgc tcgtagagga gacatcccca gcgggtatct
      601 atgaacggag cgatttcgaa atgggcgtcg aagataaaat cgacagaaat acagggctgc
      661 tctacggaga ggtacccaaa agaatagagg tgattgagaa cggattgaag ttcctggtgg
      721 acatcgaaaa gagtcagaag accgggttct ttttcgatca gagggagtct agagcctttt
      781 gttcttcgct ggtgaagaaa ttgtctctca aaaagggtct cgatcttttt tccttcacag
      841 gcgctttcgg aatcaacatg gcggccgcgg gcgcggaggt gatctgtgta gacaagtctg
      901 tggaggacct gaagctggcg aagttgaact cggagttgaa tggactggat aaatgcgttc
      961 aattcgctca gagcgatgtt attgacttta taggtcggtt tgaaaaagat gatgcatttg
     1021 atatagttgt tttggatcca ccctctctcg ttaagcacag gaaggagcta tcgaccgggg
     1081 tcggcctctt cagggacatg ataaagggcc tgctgcccct tgtcagagac agaggggtac
     1141 tcggcatttg cacatgcgcg tacaacatcg gtctggagca ccttgtcgag gcagtgagga
     1201 ggtcaacaga gaacagcggg atcacgttct cgcaccttgc ggttactctt caatctccgg
     1261 accacccttg gcttatgcag gttccagaga gtctttattt gaaatgtttg tggggcacga
     1321 ttgaaaagga gtgaagttct tgcccgggtt ctggactcac atcatctttg gaaaggacgt
     1381 tctccaggag gttggactgg atccagacag cgagagaaag ggagagttca acctcgggtg
     1441 ccttttcaca gatataggca agtactgtga aaaaaactct cctgagtatt tgctctggaa
     1501 gttatcacac acaaccgggt gcgacgcctt tgccggtgtt ctatgcaggg agctcgaggg
     1561 agtttcgagg ttctatgtta tgggggtttt ctgtcatttt tttctagatc tttccgtcag
     1621 tcagtacatc atggcgcggg ccggtaccgg gtacagaagc ggacagctcg agataatgat
     1681 agacagaacc attctcaagg agagattgac tgcggatata cttaagttga gacctctggc
     1741 ggagcttcca ccctcgatag acgaagagct cgaacggacc attcaaagaa cggccggtga
     1801 ggtctataac atcgatagcc cttcgatcac cagggccgtt gattccatga aggatatttt
     1861 tgacgattat tacggtagat ccaggttgtt tctctttttt aaagctcttt ttctgaaggg
     1921 tgaaagcatc gaaagcgctt ttttcagaga tacgcacgat gatcccatga actttggaat
     1981 gaggccctgg tttcacagca tagcgggctg ggaaagtcgg ttgactttca gggatcttta
     2041 cgggaaggcg gtttccgatg cggtcaggtt tcttcacaga tacctagatg gagaggaaga
     2101 ctggtcgctt ccggcaatgt cgttgatgac cggcttgcct cttatggaac aggaagacaa
     2161 caaaaatgtt gaagggaggg aaaccacggc ccgacactag ataagggcgc gtggtgagag
     2221 aatgaaggta ctcgggataa tcttagcagg cggcaggggt gaatacttgg gaccgctgac
     2281 acaggttcgg acgagtgctg ccctgccagt gttcggtaaa tatcgctcta tcgacttcac
     2341 tctcagcaac atggtaaatg ctggaatttc aaaagttgga ataataactc agtacagccc
     2401 cagaagcctt atggatcaca taggttctgg caaggaatgg gatcttgata gaaaacaggg
     2461 tggtctcttc atcctgcaac cttactattc cccatacaac ccgtccatgg gttacaaagg
     2521 aacggccgac gctatattcc agaacatcaa catactgagg aggggtaacg aagattatgt
     2581 gatgataggt tctggcgacc atatcttcaa agccgatctc agcgaaatct tccgctacca
     2641 cctcgagacc ggttccgaca tcactgccct gacggcgagc aatatcgaga attacaattt
     2701 cggaaatgta tctctcctga aatgcaagga cggcagagtt gtcgagtgga tcgagaggga
     2761 aggaccgctc gaagacctcg gaggctccaa ctgcgtggct ctgggagtct atttcatgaa
     2821 caagttcctg cttcgcgaac tcctctactc gaccgtgccc aacggggaga atgagctggt
     2881 gaaaggagtg ataattccca accttggctc cctaaacgtg agagaataca ggtacaacgg
     2941 ttactggcga gacataaagc agagcaagag ctgttattac cgcaccaatc tcgatattct
     3001 caaccccgaa atcagacgtg agcttttcta cgagcatggg agagtcttca cgaagctgaa
     3061 ggatcttccg ccgccaaaga taaccggtac ggcctttatt agcaactccg ttatcgccga
     3121 cggatgcgtg gtcggtggaa agctagaaga ttccgtgctt ttcagggata cacgggttct
     3181 ggcaggtgcc acggtgaaag gttcagtact tctggagggc tgtatcgtcg aagaaggggc
     3241 ttatgtggag aacgttatac tggacaagta ctgcacagtc agatcgggga agagtttcgt
     3301 aggcgagcag tccgatccca cagtgatcga gaaacacggc gttatatagg gtttgaggtg
     3361 ataatatggc gaaaaaagtg gttgctttaa tactggctgg cggccagggt accagactcg
     3421 gtattctaac agacgagatc gccaaaccgg ccgttccttt cggcggaaag tacagaataa
     3481 tcgattttgc gctcagtaac tgcgtcaact cgggtattta caccgtcgga gtgctcacac
     3541 agtacagacc gcacgttctt tcgaggcata taggtatagg aaggccctgg gatctcgaca
     3601 gaaaagacgg cggggtggtg attcttccac cttacgtggc gcaggacgat tctaactggt
     3661 acaaggggac ggcaaacgcg gtctatcaaa acatcgacta tattgatgct tacagccccg
     3721 aaatagtggt cgttctctcc ggcgatcaca tttactccat ggattataac gaaatgatag
     3781 actaccatct ctccaaaggt gcagacggca ccgtggcatg catgcgcgta cctctttcag
     3841 aggcccatcg cttcggcatg atggtcaccg attttgaaaa ccgaatcatc gatttccagg
     3901 aaaagccgga aaagccgcga tcggatctag cctcgctcgg catctacgtt ttcaactgga
     3961 gtttcctcaa agaaaggttg ctagaggacg aacagactcc ggaaagcagc aacgatttcg
     4021 gtaaaaacat actacccgaa acagttgaca gggatcttgg aaagcttttc gccttcgttt
     4081 tcgaaggcta ctggagagac gtaggaactc tccaggctct ctgggaatcc aacatcgagc
     4141 tgaccaggcc gattccgccc ctcaacctcc acgacccggc ctggaggttc tatacgcaga
     4201 ccgaagaata cctgccggcc tacgtcggga ggagttcgag ggtcaggaat tctcttatca
     4261 acgaaggagc agagatctac ggacgtatag acagttcggt gatctttcag ggtgtgcaga
     4321 taggcgaggg ttcggtcgtt cgcaattccg tgataatgac caacaccagg ataggtaaag
     4381 gcgtgattat cgacaaggca ataatcggcg agaattcggt gataaatgac gactgtgtac
     4441 tggggtacgg tgaggaagtt ccccacgaaa cgcatccggg tatatacgat tccggtatcg
     4501 tcgtggtcgg atacgacgcc gtcatcccgg ccggaacaaa gattgggaag aactgcgcga
     4561 tcgcaaatca tgtgagggaa ggagattttt cgggcgatgt acagggcgga aagacgatcg
     4621 ctgcgaagga atgatggctc tctctcttta ggtctgctga tcgcgctcct gtttgtggcg
     4681 gcagcgatga cgtccatgat gacacccttg agtctcgcaa ccagtgcttt cagagagtgt
     4741 ttgtgggagt tcaggctggc aatcacactg aaatcgatct acctatcgga cttcgaagag
     4801 gtggaaatga acggcctgaa aatcgaaaaa agctcgagaa gtgtcttctc agataaaaca
     4861 gaaatcgaga ttatccatta ccatattact gaggggagca attcctttga tttcgagaaa
     4921 gtgggaaaga taaccaacat ttcttaaaag gaggttcgat atgaagaagt ttctgttaat
     4981 cgctctgatc gtaacactat cgatttttac actcgcccag agactcacca ttctccacat
     5041 caacgacacc cacggccaca tatggcccga gggagagttc ggcgggctcg ccgccatcgc
     5101 gaccctggtg aatcaggcga ggatgtccaa tccgaacact atctttttac atgctggaga
     5161 cataaacacc ggcgtacctg aatccgacct tcaagatgcc gcgccagaca tagtcgccct
     5221 taaccttatg aagctcgatg ccatgacatt gggaaaccac gagttcgaca acgacgccac
     5281 cgttctggcc aagcagatgg atcttgctac cttcccgttc ctgagtgcca acatatacaa
     5341 agacggtaaa ccggccttca cacagtacac aatcaaggaa gtcggaggaa tcaaagtggc
     5401 gatagtcggt ttcacagctc aggaaactga aatactcgag tatctgtacg caaaggatta
     5461 tgagtggaaa gaagtagtcg aagttgctaa ggaactgata ccccagctcg aagcccaggc
     5521 cgacataatc gtagcgttga cacatatggg tagcaacccg gttctggtcg gaccgaattc
     5581 ctgggagttg gcggcggccg tcgatggcat tgacgtgatc gtggacggtc acagccacac
     5641 tttctacgag agaccggaag tgataaacgg cacattgata gtatcggccg gcgaatggag
     5701 taaatatctt gggaaattgg atctggaagt gatcaacgga acgatcactt tcgtctcctt
     5761 cagaccgatc aaagtagaga gctccatcgc tccggatttc gccgtcgcca ctgtgctcga
     5821 ttacttcaag aaggccggcg gagaggccct gaacgtggtg gtcggcgaga cgaccataaa
     5881 gctagaaggt gacagaaacg tggtcagggc acaggacacg aacctcggat accttatttg
     5941 tgatgccatg gtctggaagt ccggcgccga catcgcgata agcaactccg gtggtatcag
     6001 agcttcgata gcggccggtc ctataactta ccgcgatatt ctaaccgttc ttccgttcgg
     6061 caactctctg tacgttatcg aaatcagcgg tgccgacttc atgaaggtcc tggaattcac
     6121 tgccacgata gcggccggcc agggagcgat gccacaggtc agcggtgtca gctacacaat
     6181 cgaaaacggt accgtcaaag atgttctcgt taacggagaa ccggttgtga tggataagat
     6241 ctacaaggtt gctacaaaca actacctggc ttccggcgga gacggctata ctgccctcgc
     6301 caaccaccct ggttacgata cgggattcgt tctcgccgat gtggtagtcg agtatgtggg
     6361 ccagataagc ccgatcacat cttaccagga gagcggaagg atcaatcgcg tgaagtaatc
     6421 gtttcccagc aactgaacat tgtactataa taggagcgga gcgatccgct ccttttattg
     6481 gtcagtattg ttt
//
LOCUS       DAOOLY010000135         6474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14252_length_6474_cov_4.031158, whole genome shotgun sequence.
ACCESSION   DAOOLY010000135 DAOOLY010000000
VERSION     DAOOLY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14252_length_6474_cov_4.031158"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLD15_07570"
     CDS             complement(<1..272)
                     /locus_tag="PLD15_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPB63802.1"
                     /translation="MERTFAYLKPNAIQRGLVGEIITTLENKGLKIVALKMIEMDKYL
                     AGELYREHVGKDFFEPLMKFVQSGPVVAMVLEGENAIKRLRHTVGKT"
     gene            441..1355
                     /locus_tag="PLD15_07575"
     CDS             441..1355
                     /locus_tag="PLD15_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63803.1"
                     /translation="MIGLDFSWDRKRLTEDFLLIAPENGNIGIEKVREIEAHFLHRPP
                     AGRRKFILITQCERMTPESANALLKILEEPPDFGRIILNTTSWNSLLATIRSRVIPIN
                     IEVPDVIMEEIKRKYRDKVIEIYAGSIEDYSILSYFRDLEAEALTVDALELPEGENCV
                     EYLEDVENEDPIFKIKRRKAYMNLIKRFIAENDFFALAGRIAPGFGGVGSFEKCRLLV
                     FYARGLLRDLLVVSRTSLWSRVVNIDLIEWLMEYREGPNTLEDFLWCDRFLRRRTASL
                     NPTLVFFRAMYSVRRNFDRDAAGGRMNA"
     gene            1348..2232
                     /gene="ricT"
                     /locus_tag="PLD15_07580"
     CDS             1348..2232
                     /gene="ricT"
                     /locus_tag="PLD15_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPB63804.1"
                     /translation="MPEIYGTVYGVEFHTVGKMYEYSSSEGVLKTGDFVLAMSEFGLD
                     VGKVRFGPVDMRIDELKEELRPIVRMMTDEDWETYNKNKEEAISAMRICQDLIEKHSL
                     PMRLLEARYMFDRSRIVFYFGADSRVDFRELVKDLARAFRTRIELRQVGIRDEVKMTG
                     SLGLCGMTACCARFLRQFESITLKHAKKQQLLINPAKISGRCGRLLCCLSYEQELYEH
                     ELMDIPDEGSLVDYEGKTCKVLTVNIFMKVITLVADDGQMIKVQFDDFRKSQKSIIQD
                     ANAELLIKNRDEDISMDD"
     gene            2251..4149
                     /locus_tag="PLD15_07585"
     CDS             2251..4149
                     /locus_tag="PLD15_07585"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB63805.1"
                     /translation="MTGNAERGIEELKKLGRICRGDIIKMTTIANSGHPGGSMSSIDM
                     YLSIYNKANINPSRPYDPNRDRVIVSHGHTSPGVYAALGRLGFFNIDDVISGFRHAGS
                     IFEGHITRGIPGVEWTTGNLGQGLSVGVGMALASKLTGRNFRVFVAMSDAEQAKGQVS
                     EARRTAVKYRLNNLVVVIDYNDAQISGRARDIMYVDIKANYLADGWDVIEVNGHDYHQ
                     IMEALSRSFGGEKPVAVICRTVMGKGISFMEGDVSYHGKPLSFETAAKALEELGLEND
                     LDRYAEMRKALPSHHEPVKPVDEPIKPAIGTPITYPVDKKTDNRSAFGKALADIGKTN
                     RNGTPVVVFDCDLKPSTKVNEFEKVWPENFIQIGVQEHNAAVVAGAASVCGTLSFFAD
                     FGVFGIDETYNQQRLNDINKANVKVAVTHVGIDVGEDGKTHHCLDYIGALRNFFGFGL
                     IVPADPNQTDRAVRYMSQTQGNCVIAMGRSTMNPIADEKGGPFFGDSYKYEYGKIDVI
                     RKGKGITLLSTGQVTHQAVLAADQLKKDGLEVTVLNLSAPLASDLDSARDYLVGTVIT
                     LEDHNVNTGLASIVEEYFVREGFLPEKFVKIGVDRYMYSGDNEKLYDLVGLSRDSIVE
                     KVREILRQ"
     gene            4149..4883
                     /gene="rnc"
                     /locus_tag="PLD15_07590"
     CDS             4149..4883
                     /gene="rnc"
                     /locus_tag="PLD15_07590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB63806.1"
                     /translation="MNQEEEKKVIEFCSINSISADRELIFKALCHSSFANELAQNTSR
                     ILESNERMEFLGDAVLEFSLATLLYRNFALSEGEMSKIRAMVGSEKVLSEVARELRIG
                     EYLFLGKGERQTGGADRESILADAFEALLAAIYLSYGIETALNFVRERLYRYVNKALE
                     GALVLDYKTSLQEFTQASFGSRPSYTTIQEDGPPQDKLFKVEVVVEDRLLGVGEGRTK
                     KAAEQSAARKALEALLKEEIDRENSP"
     gene            4880..5695
                     /locus_tag="PLD15_07595"
     CDS             4880..5695
                     /locus_tag="PLD15_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPB63807.1"
                     /translation="MKYRHLVVGIKEEQRDLFEAWSWEEGFMNLYFEELPDMTVTLHV
                     ILAEGEELPEFLTEVEFVDLGFSSEEDWYEKWRQTLRPFSLCGGITVVPMEEERPVES
                     LSEIGLIPGMAFGTGLHESTRLAASLIKKVVRSGDRVLDVGCGTGILSVLAMKCGASE
                     AIALDIDEHALEKTRETARINGVSVEVVKSDFLSALSEGESFDLIIANMIAELLERFA
                     GDMKKFLRKSGSIVLSGIYREKIRSISKLIDEDFTIDDTEEDGDWKALLLKER"
     gene            5665..6201
                     /locus_tag="PLD15_07600"
     CDS             5665..6201
                     /locus_tag="PLD15_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPB63808.1"
                     /translation="MESASPEREIVSLQLDRELKNDFSTARFCKWGYPQVIRSELISG
                     AKPFPTLFWLTCPLLRRLVSTMESGGLIQEFEYRIERESVYRERYLKAHEETALMKRE
                     SLEGLELSEWQKEKLFGSGIGGIANLLKVKCLHLQLANYLGGVSNPVGRDVWESLGEK
                     ECSGERVICLELLRRNER"
     gene            6191..>6474
                     /locus_tag="PLD15_07605"
     CDS             6191..>6474
                     /locus_tag="PLD15_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HPB63809.1"
                     /translation="MNDSLLERVSQLPEEPGVYIFKSDKDETIYVGKAKKLRRRVQSY
                     FRESTWSKNEKVRNIAVEASDLQFILVTSEREALLLEANIIFKEKPKFNVL"
BASE COUNT         1774 a         1426 c         1806 g         1468 t
ORIGIN      
        1 gtctttccta ctgtatggcg gagcctcttt atggcgttct caccctcgag caccatagct
       61 acgacaggtc ccgattgaac aaacttcata agcggttcga agaagtcttt gccgacatgt
      121 tctctgtaaa gctcaccggc caggtatttg tccatttcta tcattttgag ggctactatc
      181 ttaagacctt tgttctcgag agtggtgatt atctcgccga ccagacccct ctggatagcg
      241 ttcggtttta gataagcgaa tgttctctcc atcttttcga cctccgggat aattgaacca
      301 ttcattgcta taattaaatc atggactcta ccaatagaga tttaagagag agactttccc
      361 gtatcctggc gggaagtgcg ggcttaagta tagcacttac tggaagagat tgccgttatc
      421 tcaagacctt ctcagaggat ttgattggtc ttgacttttc ctgggacaga aaaaggttga
      481 cagaggattt tctgttgatt gcgccggaga acggaaatat cggtatagag aaggttcgcg
      541 agatcgaagc tcattttctc cacaggccgc ccgcgggacg gcgaaagttc atcctcataa
      601 ctcagtgcga gcgaatgacc ccggagagcg ccaacgcttt gcttaagata ctggaagagc
      661 ctccggactt cgggagaatc atcctcaaca ccacttcctg gaactcgttg cttgcgacta
      721 tacgcagccg tgtaatcccc ataaacatcg aagtgccgga cgtgataatg gaggaaataa
      781 aaaggaaata cagagataaa gtcatcgaga tctacgccgg ctctatagaa gattactcga
      841 tcctgtctta cttcagagac ctcgaagcgg aggctttgac tgtcgatgct ctcgagctcc
      901 cggaaggtga aaattgtgtc gagtatctgg aagacgtgga aaatgaagac cccatcttca
      961 aaatcaaaag aagaaaggct tatatgaatc tgataaagcg cttcatagcg gagaacgact
     1021 ttttcgcgct cgccggaagg attgcaccag gattcggcgg cgttggttcc ttcgaaaagt
     1081 gtcgtcttct ggtgttctac gcgagaggac ttctgcgaga tctgctggtc gtctcgagga
     1141 cttcgctctg gagtcgagtt gtcaacatag atctgataga atggcttatg gaatacaggg
     1201 agggaccaaa tacgttggaa gatttcctct ggtgcgatag attcctgaga agacgcaccg
     1261 cctctcttaa cccgacactc gtctttttca gggctatgta ttcggtcagg aggaatttcg
     1321 atagagacgc tgctggaggt agaatgaatg cctgagatat acggaaccgt ttacggagtt
     1381 gaatttcata cggtcggaaa aatgtacgaa tactcctcga gcgaaggggt tttgaaaact
     1441 ggagacttcg ttctggctat gagcgagttc ggactggatg ttggaaaagt cagatttggc
     1501 ccggttgata tgcgtatcga tgagcttaaa gaagaactgc gaccaatcgt gcggatgatg
     1561 acggacgaag actgggagac gtacaacaag aataaggagg aggcgatctc ggccatgcga
     1621 atctgtcagg atttgatcga gaagcactct ctgcccatgc gcctgctgga ggcgaggtac
     1681 atgttcgatc gttcgcgtat cgtattctac ttcggggccg acagccgggt ggattttcgc
     1741 gagctggtca aggacctggc aagagctttc agaacgagaa tagaacttcg ccaggtcggc
     1801 ataagagatg aagtcaaaat gaccggcagt ctaggccttt gcggcatgac ggcctgctgt
     1861 gccaggttcc taaggcagtt cgaaagtata actcttaaac acgcgaagaa acagcaacta
     1921 ctgataaatc cggcgaagat atcgggccgt tgcggtagat tgctctgctg tctctcgtat
     1981 gaacaggagt tgtacgaaca tgaactgatg gatatacccg acgagggttc tctagtggat
     2041 tacgagggaa agacctgcaa ggtgttgacg gtgaatatat tcatgaaggt gataactctg
     2101 gttgccgacg atgggcagat gataaaggtc cagttcgacg attttcgcaa gagtcagaaa
     2161 tccataatac aggacgccaa cgccgagttg ctcataaaga acagagatga agatatttca
     2221 atggacgatt gaattgagtg gaggtgtgaa atgacaggaa acgctgaaag gggaatagaa
     2281 gaactcaaaa aactcggaag gatatgcagg ggagacataa taaagatgac aacgatcgcc
     2341 aactccggtc accccggggg atcgatgtct tcaatagata tgtacctgtc tatatacaat
     2401 aaagcaaata taaatccttc aagaccgtac gatccgaaca gggacagggt catcgtgagc
     2461 cacggccaca cctctccggg agtttatgct gctttaggaa gactgggttt ttttaacatc
     2521 gacgatgtca tatctggctt caggcacgca ggttccatat tcgaaggaca tatcaccagg
     2581 ggaattcccg gtgtggaatg gacgaccggt aatctcggtc aggggctctc ggtaggtgtc
     2641 ggcatggctc tggcctcgaa gctgaccggc agaaacttca gggtcttcgt ggccatgagc
     2701 gatgccgaac aggccaaagg acaggtgagc gaggccagaa gaacggccgt caagtacaga
     2761 ttgaacaacc tggtcgtggt catagattac aacgatgccc agatatccgg cagagcacga
     2821 gatatcatgt acgtcgatat caaggccaat tatcttgccg atggctggga tgtgatagaa
     2881 gtcaatggac acgattatca ccagataatg gaggctcttt cgaggtcctt cgggggcgaa
     2941 aaacccgttg cagtgatctg cagaactgtt atgggaaagg ggatctcctt catggagggc
     3001 gatgtctcct atcatggaaa acctctcagt tttgaaacgg ccgcgaaagc cctcgaagag
     3061 ctcggacttg aaaatgatct tgacagatac gcggaaatga gaaaggcttt accctcccac
     3121 catgaaccgg ttaagccggt cgatgagccc ataaagcccg ctatcggcac accgatcact
     3181 tatcccgtgg acaagaagac agacaacaga tcggctttcg ggaaggcgct ggccgatatc
     3241 ggaaagacca acagaaacgg cacccccgtg gtggttttcg actgcgactt gaagccttcg
     3301 accaaggtga acgaattcga gaaggtctgg ccagagaact tcatacagat aggagtgcag
     3361 gaacacaacg cggcagtcgt agcaggcgcc gcctctgtat gcggcactct cagctttttc
     3421 gcagattttg gagtcttcgg aatcgacgag acctacaacc agcagagatt gaacgatata
     3481 aacaaggcca acgttaaagt ggccgtcacg cacgtgggaa tcgatgtcgg tgaggacggg
     3541 aaaacccacc actgcctgga ctacataggg gctttgcgga acttcttcgg tttcggtctg
     3601 atcgttcccg ccgaccccaa ccagaccgac agggccgtga ggtatatgag ccaaacgcag
     3661 ggtaactgcg taatcgccat gggaaggtca accatgaatc ccatagccga tgagaagggc
     3721 ggacccttct ttggagacag ttacaaatac gaatatggca agatagatgt tatcagaaaa
     3781 ggaaagggta taacgctcct atcgaccggc caggtcacgc accaggcagt tctggcggcc
     3841 gatcaactga agaaggacgg tctggaagtc acggtgttga atctcagcgc cccgctggct
     3901 tccgatcttg atagtgctag agactacctg gtgggaacag ttataacgct cgaggatcac
     3961 aacgtcaaca cgggactggc ttcgatagtc gaagagtatt ttgtgagaga gggatttcta
     4021 ccagagaagt tcgtcaagat cggtgtcgat agatacatgt actccggcga caacgaaaag
     4081 ctgtacgatc tggtggggct ttccagagac agtatcgtcg aaaaggtgag ggaaatcctg
     4141 agacaatgat gaaccaggaa gaagagaaaa aggtgattga attctgttcg atcaactcga
     4201 taagcgccga tcgcgaattg attttcaaag cattgtgcca ctcatctttc gccaacgagc
     4261 ttgcgcagaa cacatccagg atactcgaat ccaacgagag aatggagttt ctgggagacg
     4321 ccgttctgga gttttctctc gcgacgcttc tttacaggaa ttttgccctc tcggaaggcg
     4381 aaatgtcgaa aatcagggcc atggttggca gtgagaaggt tctttccgaa gtcgccagag
     4441 agctcaggat cggtgagtac ctctttctcg gaaaagggga aagacagacc ggtggggcag
     4501 acagggaatc gatactcgcc gacgctttcg aagcgcttct ggcggcgata tatctgagct
     4561 atggcataga aacggccctg aatttcgtca gagagcggtt gtaccgctac gtgaataaag
     4621 cgttggaagg cgccctggta ctggactaca aaacatctct tcaggagttc acacaggcca
     4681 gtttcggctc gcggccctct tatacgacga ttcaggaaga cgggccaccg caggataagt
     4741 tattcaaagt ggaggtagta gtagaagata ggcttctagg agttggggaa ggcaggacga
     4801 agaaagccgc cgagcagtcg gccgcaagga aggctcttga ggcgctcctt aaggaagaga
     4861 tagatcggga gaattcacca tgaagtacag gcatcttgtt gtcggaatca aagaagaaca
     4921 gcgggatctt ttcgaagcct ggagctggga agagggcttc atgaatcttt atttcgagga
     4981 attgcccgac atgacagtaa cgcttcacgt tatactcgct gagggcgagg aactgccgga
     5041 gtttctcaca gaggtggagt tcgtagatct aggcttttcc agtgaagagg actggtacga
     5101 aaagtggcgt cagactctgc ggccattttc tctctgtggt gggatcacag tagtcccgat
     5161 ggaggaggag agaccggtcg aaagcttgag cgaaatcggc ctgataccgg gaatggcttt
     5221 cgggacggga cttcacgaat ccaccagact ggcagcctct ctgataaaaa aggtcgttcg
     5281 ttccggtgac cgtgttctag atgtggggtg cgggacggga atactctcgg tactggccat
     5341 gaaatgcggc gcttcggaag ctatcgcgct ggatatagat gaacacgcgc tggagaagac
     5401 gcgcgagacg gccaggataa acggggtttc cgtcgaagtc gttaaatccg atttcctttc
     5461 ggcgctttca gaaggcgagt ctttcgatct gataatcgcc aacatgatcg ccgaacttct
     5521 ggagcgcttc gccggagaca tgaagaaatt tttgaggaaa agcggttcga tagtgctctc
     5581 aggaatttac agagagaaga tacgatcgat ttcgaaactc atcgatgaag attttacaat
     5641 cgatgataca gaggaagatg gcgattggaa agcgcttctc ctgaaagaga gatagtatct
     5701 ctccagctgg atagagaatt gaagaacgac ttctcaactg ccaggttctg caagtgggga
     5761 tatccacagg taattcggtc ggagctgata tctggagcga aacccttccc gactcttttc
     5821 tggctgacct gtcccctgtt gagaaggttg gtctcgacta tggaaagcgg aggattgatt
     5881 caagaattcg aatacaggat cgagagagag agcgtctacc gcgaaaggta tctgaaagcc
     5941 cacgaagaaa cagctctcat gaaaagagag agcctggagg gattggagct ttctgagtgg
     6001 cagaaagaga agttgttcgg gagtggaata ggcggtatag ccaatctgct caaggtcaag
     6061 tgtctccacc tgcaactggc caattatctt ggcggggttt caaaccccgt tggaagagat
     6121 gtctgggaat ctttgggcga aaaggaatgt agtggtgaaa gagtcatctg tctggagttg
     6181 ttgaggagaa atgaacgata gcttgctcga aagagtgtcg cagctgcccg aagaaccggg
     6241 cgtttatata ttcaaaagcg acaaagacga aacgatatac gtcggaaagg ccaagaaact
     6301 ccgtcgccgt gtccagtcgt acttcagaga atccacctgg tcgaaaaacg aaaaagtcag
     6361 gaatatagcc gtcgaagcct ccgatctcca gttcattctc gtaacttcgg aaagagaagc
     6421 gctcttactg gaagcgaata tcattttcaa agagaagccc aagttcaacg tact
//
LOCUS       DAOOLY010000136         6446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14344_length_6446_cov_4.994054, whole genome shotgun sequence.
ACCESSION   DAOOLY010000136 DAOOLY010000000
VERSION     DAOOLY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14344_length_6446_cov_4.994054"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1810)
                     /locus_tag="PLD15_07610"
     CDS             complement(32..1810)
                     /locus_tag="PLD15_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63810.1"
                     /translation="MTVSEDIRKWLVSSGVLTRVGKPSRYIGKELNAVLKDTMGKLRV
                     LLAFPDAYEVGMSHLGFKVLYRELNSRDSTFAERTYLPWKDMIDEMRGNSVPLFSLET
                     YSPALEFDIVGITLQYELGYTNVLALLELAGIPLFQRDRTHEPIVLGGGPCASNPEPM
                     SDFFDAFVIGDGEEAMLEVCGLIEKNLDLLRSGKRRELLEIISEVRGVYIPSAGKRNV
                     VKAAVADISDYGIDGRPVVPFMKVIHDRAIVEVMRGCNRGCRFCQAGIFYRPVRERVK
                     EEIAEGVREILRGTGYDEVSLLSLSTMDHSQIGELTDSLLPLMEPRKIALSLPSSRID
                     AFDVEIASRIASVRKTGLTFAPEAGTQRLRNVINKNVTEKDLLDCAVIARKKGWQRLK
                     LYFMIGLPTETSEDIEGIVKLSRRVKAIGFKELAVSVAVFIPKPHTPFQFYRQIDENE
                     ARQKAEFLRGFSGRGIDIKVHNPSNGPVEGILSRGDRKVGRAVYEAYRLGAIFDEWDD
                     QFDRRIWDEALKIASIDTEGYFKGYSTDETLPWEHISLGVSKKYLVSEYFRSLKEQTT
                     PDCRWTGCTDCSVCGNLGISNVLRKV"
     gene            complement(1807..2808)
                     /locus_tag="PLD15_07615"
     CDS             complement(1807..2808)
                     /locus_tag="PLD15_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB63811.1"
                     /translation="MDLDRLTEVVFVPGITGREEMIRSKILEMIPQEIEPQIDDLGNV
                     TIEIGQGDSSIAFAAHMDEIGLLITGVNSDGTLNFKKLGGVPDELLVGRHMDVITSSG
                     ESIDGVIGITPPHLKEKTDLSWQSLSIDLGASSKEEAESMGIEVLDYAVFRKQISILN
                     EKFVAVRSLDDRFGCLALLDLLDLLVKIDLPRKVLFSWTVQEEIGLIGARALSERIKP
                     SLFFPVDSFACCSRLTGDVRPGNGPVLRMSDTSSLGDYQTGRKIVEHARDKGLPLQVG
                     VTGGGTDGIPFQQHGVKMVPLSLAVKNLHSETEYISMEDYDNLVKLMFSLATEFQIP"
     gene            3114..4784
                     /locus_tag="PLD15_07620"
     CDS             3114..4784
                     /locus_tag="PLD15_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB63812.1"
                     /translation="MNNPENLKKVKEEWKEKKVLPLLRKFPERKSQFKTSFDGDIDIL
                     YTPEDLPPDGYDQALGFPGQYPFTRGVQPTMYRGRFWTMRQYAGFGTAEESNERYRYL
                     LSQGQTGLSVAFDLPTQIGYDSDDQMAEGEVGKVGVAIDSLEDMEVLFEGIPLDKVST
                     SMTINSTAAILLAMYIAVGEKQGVDPSRLTGTIQNDILKEYIARGTYIFPPDASMRLI
                     TDIFEYCSKALPDFNTISISGYHIREAGADAAQEVAFTLADGIAYVESAIKAGLDPNV
                     FGKRLSFFFNSHNNFLEEIAKFRAARRLWAKIMKDRFGVTDEKAMMLRFHTQTGGSTL
                     TAQQPMNNIIRVAFQALAAVLGGTQSLHTNSFDEALGLPTQDSVTIALRTQQIIACET
                     GVTDTVDPVGGSYYVENLTDRIEKLALDYLQKIDDLGGMVKAVQEGYIQKEILNTAYN
                     YQLKVEREEQVIVGVNQYLSDENLKKDILKIDPVIEEKQRNKLAALKRRRNSQVVEEA
                     LKELKKAALGSENLFPIILNAVKSYATLGEISNTLRDVFGEYTEAVIL"
     gene            4802..5194
                     /locus_tag="PLD15_07625"
     CDS             4802..5194
                     /locus_tag="PLD15_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPB63813.1"
                     /translation="MKRVKVLVAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSAD
                     EVVKAALQEDVDIIGLSILSGAHMRICEKVLNLMKEKGIDEKPVFVGGIIPAEDALEL
                     KKMGILEVFGPGTPLKAIVDSIKGAVDK"
     gene            5191..6060
                     /gene="meaB"
                     /locus_tag="PLD15_07630"
     CDS             5191..6060
                     /gene="meaB"
                     /locus_tag="PLD15_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB63814.1"
                     /translation="MSERLFYANQLAISRTLTRIENDRSVAREIIGSLEERDYHVVGI
                     TGSPGAGKSTLTDRLALLSSKEKRTAIIAIDPSSPFTGGAFLGDRIRMKSSTKSEEIF
                     VRSMASRGKVGGLSPSIYDAVEFLGRSGFDRVFVETVGAGQSETDIENLADVVLLVLA
                     PGLGDDVQTMKAGIMEIGDIFAVNKMDLPGASQLATRTGAILEMSGRELPVILTNSIN
                     GQGVEELHNLIEKALGELDISGAIEEKRRRRTLYNNLNSVYQIIKEDFSENAKLEELM
                     NYLLENRRLSDES"
     gene            6050..>6446
                     /gene="mce"
                     /locus_tag="PLD15_07635"
     CDS             6050..>6446
                     /gene="mce"
                     /locus_tag="PLD15_07635"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPB63815.1"
                     /translation="MRADRIDHIGVAVKSIEERLKVYRDLLKLDPVKIEELPERGLRV
                     AFVNVGDTRIELLEPIAENSQVSKFLETRGEGIHHMAFHVDDIEAAIKEAVELEIVPL
                     TKEAQPGASGTRVVFLHPKSAGGVLIELVE"
BASE COUNT         1677 a         1629 c         1661 g         1479 t
ORIGIN      
        1 gacattacag aagcgtcaaa aatgagtgct ctcaaacttt gcgcaacaca ttagatattc
       61 ccagatttcc acataccgaa caatccgtgc atccggtcca cctgcaatca ggtgtagtct
      121 gttctttcag tgacctgaag tattcgctca ccagatattt ctttgagaca cccagcgaga
      181 tatgttccca gggtagcgtc tcgtcggtag agtaaccctt gaagtaaccc tccgtatcga
      241 tcgaggcgat ttttaaggct tcgtcccaga tcctccggtc gaactggtcg tcccattcat
      301 cgaatatcgc tccaagtctg taggcctcgt aaacggctct ccctactttt ctatcgccgc
      361 ggctcaaaat accctcgacc ggcccgttcg agggattgtg aactttaatg tctatacccc
      421 ttccggaaaa gcccctcaag aattcggcct tctgacgggc ctcgttttcg tctatctgcc
      481 tgtaaaactg aaacggcgtg tgcggttttg gtatgaaaac ggcaacagat acggccaatt
      541 ctttgaatcc gatcgccttg acccttctgc tgagtttcac tatcccttcg atatcttcgg
      601 aagtttctgt gggaaggccg atcatgaagt acagcttcaa cctctgccag ccttttttcc
      661 tggctatcac agcgcagtcc agaagatctt tctcggtcac gttcttgttt atgacatttc
      721 tcaacctctg cgttccggcc tcaggtgcga aagtcagacc ggtcttcctt acggaagcta
      781 ttctggaagc gatctccacg tcgaaagcgt cgattctaga actcggtaga gagagggcaa
      841 tcttacgggg ttccattagc ggcaaaagag agtcggtaag ttcgcctatc tgagagtggt
      901 ccatcgttga gagcgagagc aaggaaacct cgtcataccc ggtccctctc aatatttctc
      961 tcactccttc cgcgatctct tccttcactc tctcgcggac tggccgataa aagatcccgg
     1021 cctggcaaaa cctgcaaccc ctgttacagc ccctcatcac ttcgacgatc gccctgtcgt
     1081 gtatcacctt catgaaaggc accacgggcc ttccatctat tccgtaatcg gagatgtcgg
     1141 caacggcagc ctttacgacg tttctctttc cggccgaggg aatataaacg ccacgaactt
     1201 cggagatgat ctccagaagc tccctccttt tccccgatct gagaagatcg aggttcttct
     1261 cgatcagtcc acacacttcc agcatcgcct cttcgccgtc tccgataacg aaagcgtcga
     1321 agaagtcaga catcggttcg ggattggaag cgcatggccc gcccccgaga acgataggct
     1381 cgtgcgttct atctctctga aacagaggta tccccgccag ttcgagaagc gcaagaacgt
     1441 tcgtgtaccc gagctcgtac tggagcgtga taccgactat gtcgaattcc agagccggag
     1501 agtaagtctc cagagagaaa agcggcacgg aattccctcg catttcgtcg atcatatcct
     1561 tccagggaag ataagttctc tcggcgaagg tagagtctct ggagttcaac tctctgtaaa
     1621 ggactttgaa acccaggtgc gacattccca cctcgtaagc gtccggaaag gccagcagga
     1681 ctctcagctt tcccatcgta tctttgagga ccgcgttcaa ctccttacca atataccggc
     1741 tgggctttcc tacacgcgtc aggacgcccg aggataccag ccacttcctt atatcttcag
     1801 agacggtcat ggaatctgaa actctgtggc tagagagaac atgagcttga cgaggttgtc
     1861 ataatcttcc atagatatgt attcggtctc cgagtgaagg ttcttaacgg ccagagaaag
     1921 aggcaccatc ttcacgccgt gctgttgaaa aggtattcca tcggttccgc cgccggtaac
     1981 tccgacttga agtggaaggc ctttatccct ggcgtgttcc actatttttc tgccggtctg
     2041 gtaatcgccc agagaagatg tgtccgacat tcgaagcaca ggcccgttgc ccggcctgac
     2101 gtctccggtg agtctggaac agcaggcgaa agagtcgacg ggaaagaaga gcgaaggttt
     2161 gattctctcg gagagggccc gtgcaccgat caatcctatc tcttcctgca ccgtccagga
     2221 aaacaacacc ttcctgggaa gatcgatttt cacgagaaga tcgagaagat ccagaagcgc
     2281 gagacagccg aacctgtcat cgagagacct caccgccaca aacttttcgt tcaggatcga
     2341 tatctgcttt ctgaaaacgg cgtaatcgag aacttctatg cccatagact cggcctcttc
     2401 cttcgaagaa gctcccaagt ctatcgagag actctgccaa gacaggtccg tcttctcctt
     2461 aaggtgggga ggggtgatgc ctattacacc gtctatggac tctccggagg aagttatcac
     2521 gtccatatgt cgccccacca gaagttcgtc gggaacaccg cctagcttct tgaaattgag
     2581 tgtcccgtcg ctgttaactc ctgttatcag aagacctatc tcatccatgt gcgctgcgaa
     2641 ggcgatactg gaatcgccct gacctatttc gattgtgacg tttcccagat catctatttg
     2701 cggttctatc tcttgaggta tcatttcgag aattttggac cttatcatct cttcgcggcc
     2761 ggtgattccc ggaacgaaca ccacttctgt caatctgtcg agatccatga gattacctcc
     2821 ttgtggtagt caaccattgc gtcatattta tagaccgtcc cgaaaacgga actctcgcgg
     2881 gattgtggcc gatccttcgg cgatggaagt ctgagcgttt taccgcgcat atcttttctc
     2941 ccactcgttc gatttcctcc ctattatagc ctacggaaaa gaagatatga acttgagtcc
     3001 acaaaggagg tctggccagt tataatcggt attgatcaca attgacgggt ggctgtattt
     3061 gatttatctt gaatatgaaa gatctataaa gacttcgaaa tggaggatga gaaatgaaca
     3121 atccagaaaa tctcaagaaa gtcaaagaag aatggaagga aaagaaggtt ttgccactgt
     3181 tgaggaaatt ccccgagaga aagagtcaat tcaaaacgtc gttcgacggt gatatcgata
     3241 tcctctatac ccccgaagat cttccgcccg acggctacga ccaggcgctt ggctttccgg
     3301 gacagtatcc gttcacgcga ggtgtacaac cgacgatgta caggggaagg ttctggacta
     3361 tgaggcaata cgccggattt ggaacggcgg aagagtcgaa cgaaaggtac agatatctcc
     3421 tctctcaagg tcagaccggt ctttcggtgg ccttcgatct ccccacacag atcggttacg
     3481 attcagacga tcagatggcc gagggagagg tcgggaaggt gggcgtcgcc atcgactcgc
     3541 tcgaagatat ggaggttctt ttcgagggga taccgctcga caaggtaagt acgtctatga
     3601 cgataaactc cacggcggcc atacttctgg ccatgtacat agccgttgga gaaaagcagg
     3661 gagtcgatcc ctcgagatta acgggaacga ttcaaaacga tatattgaag gaatacatcg
     3721 ccagaggaac ctacatcttt ccgccagacg cttcgatgag actgataacc gatatattcg
     3781 aatactgttc gaaggcacta ccggatttca acactataag cattagcggg taccacataa
     3841 gagaggcagg cgcagatgcc gctcaggaag tggctttcac gcttgccgac ggtatagcct
     3901 acgtagaatc tgcgataaaa gccggtctgg atccaaatgt ttttggaaag aggctttctt
     3961 tcttcttcaa ttctcacaac aacttcctcg aagagatcgc caaatttcgt gccgcacgaa
     4021 ggctctgggc gaagataatg aaagacaggt tcggtgtcac cgacgaaaaa gccatgatgc
     4081 tcagattcca cactcagacc ggcggttcta ccctcaccgc acagcaaccg atgaacaata
     4141 taatcagggt ggctttccag gccctggccg ctgttctggg cggtacgcaa tcccttcaca
     4201 ccaactcctt cgacgaggcg ctcggcctgc cgacacagga ttcggttacc atagccctca
     4261 gaacacagca gataatcgcc tgtgaaaccg gagtaacgga cacggtagat ccggtaggag
     4321 gatcttacta cgttgaaaac ctgaccgaca ggatcgagaa gctcgcgttg gattacctcc
     4381 agaagatcga cgatctcggg ggaatggtca aggccgtcca ggaaggttac atacagaagg
     4441 agattctgaa caccgcttac aactaccagc tcaaagtcga aagagaagag caggtgatcg
     4501 tgggtgtaaa ccagtacctc tccgatgaaa atctgaagaa ggatattctc aaaatcgatc
     4561 cggtcatcga agagaagcag agaaacaaac tggctgcgct caaacgaagg cgaaattcac
     4621 aggtggtcga agaggccttg aaggaactca aaaaagccgc gctgggctct gaaaacctct
     4681 tccctatcat actgaatgca gtcaaaagtt atgcgacact cggagagatt tcaaataccc
     4741 tcagggatgt cttcggagag tacaccgaag ccgtcattct ttgagataaa gaggtgaaat
     4801 gatgaaaaga gtaaaggtac tcgttgcaaa accgggactc gacggacacg acaggggcgc
     4861 taaagtagtg gcgatggcgc tcagggatgc cggcatggaa gtgatctaca ccggcctcag
     4921 gcaatcggcc gacgaggtgg tgaaggctgc ccttcaggaa gacgtggata taatcggtct
     4981 ctccatcctt tctggtgcac acatgaggat ctgcgagaag gttctcaatc tcatgaagga
     5041 aaaggggata gatgaaaagc ccgtcttcgt ggggggtata ataccggccg aagacgctct
     5101 ggagctaaag aagatgggga ttctcgaagt cttcggtcca ggaacgcctt tgaaggcaat
     5161 agtcgattcg ataaagggtg cagtagataa atgagcgaga ggctttttta tgcaaatcaa
     5221 ctcgccatct cgagaacttt gaccagaata gagaacgaca gatcggtcgc cagagagatc
     5281 ataggttcgc tggaagagag agattatcac gttgtgggta tcacgggcag ccccggtgcc
     5341 ggaaaatcta cccttaccga ccggctcgcc ctcttgagtt ctaaagagaa aagaactgcg
     5401 ataatcgcca tcgatccttc aagccccttc acaggcgggg ccttcctggg agacaggata
     5461 aggatgaaga gctccacgaa gagcgaagag atattcgtga gatccatggc cagccgcggt
     5521 aaggttggcg gcctgagccc ttcgatctac gatgccgtcg agttcctcgg ccggagcggt
     5581 ttcgacaggg tgttcgtcga aacggtcgga gccggccagt ccgaaacgga tattgagaac
     5641 ctggccgacg tggttctgct ggttttggcg ccgggtttgg gagacgatgt tcagacgatg
     5701 aaggcgggaa taatggagat cggggatatc ttcgcggtaa acaaaatgga cctgcccgga
     5761 gcgtcacagc ttgcgacgag gacaggtgcc attcttgaaa tgagcggcag agagcttccg
     5821 gtaattctga ccaattcgat caacggtcag ggagtggaag aacttcacaa cctcatcgaa
     5881 aaggctctgg gagaactcga tatcagcgga gcgatcgaag agaagaggcg ccggcgtaca
     5941 ctttacaaca atttgaacag cgtgtaccaa atcataaagg aagacttctc ggaaaatgct
     6001 aaactcgaag agttaatgaa ttatcttcta gaaaacagga ggttatctga tgagagctga
     6061 caggatagac cacatcgggg tcgccgttaa atccatcgaa gagaggctga aggtttacag
     6121 agatcttcta aaactcgacc cggtgaagat cgaagaactc ccggaaaggg gtctgagagt
     6181 ggcttttgtg aatgttggcg acacacgaat cgaactgctc gaaccgatcg cggagaacag
     6241 ccaggtatcg aaattcctgg agacgagggg cgaggggata catcatatgg cctttcacgt
     6301 cgatgatatc gaagccgcca tcaaagaggc cgttgagctg gagatagtcc cgctcacgaa
     6361 ggaggctcag cccggagctt caggtacgag ggtggtattt ttgcacccca aaagtgccgg
     6421 tggtgttttg atagagctcg ttgaag
//
LOCUS       DAOOLY010000137         6392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14504_length_6392_cov_4.520751, whole genome shotgun sequence.
ACCESSION   DAOOLY010000137 DAOOLY010000000
VERSION     DAOOLY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14504_length_6392_cov_4.520751"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..832)
                     /locus_tag="PLD15_07640"
     CDS             complement(200..832)
                     /locus_tag="PLD15_07640"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HPB63816.1"
                     /translation="MSGEILKATFETLYMILVSGFLATLFGIPLGVALFLLSRSKKWR
                     RLYIVFDLLVNVFRSIPFIILVLLLIPVTKNVMGTIIGPNAAIFNLTIAAIPFMARLS
                     ENSFNGVPRGIIDSSDSMGLNRWQFIFRVLIPETLPEQIGNITVLLINLVTYTAIAGA
                     VGAGGLGQLAINYGYYRFQWDVVLYAVIVLVSISQVLQFTGGRLSRLLRR"
     gene            complement(832..1821)
                     /locus_tag="PLD15_07645"
     CDS             complement(832..1821)
                     /locus_tag="PLD15_07645"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HPB63817.1"
                     /translation="MILAVKNLNLSFEDRAGRRKILDDVSFSVEEGQIVGIVGLSGAG
                     KTSLLRTLNLLQPVDSGEIFFDGKNIVNLKDEEIRKMRKKIGVVFQSFNLFRNRTVSQ
                     NIAFPLKIQGLGSREVKEKVEKISGELGLDQRIQAYPSQLSGGEQQRAAIARALVMDP
                     KMILLDEPTSALDPKTTGRLLDLVVELNQKHRITFVVVTHDMDVIKRTCDKVAYLENG
                     KLKFFGPAHEFFVKVEKNEVRDFGTPLELDRKAIEHRGKIIRVLFWGKKTHEAVMWKV
                     ARELDVSVNILYGKIEELKHGPFGAMIIGIDGEQQDLFIQRLRESVYSLEEVQ"
     gene            complement(1836..2615)
                     /locus_tag="PLD15_07650"
     CDS             complement(1836..2615)
                     /locus_tag="PLD15_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPB63818.1"
                     /translation="MKKLLLVWVLLGLVLAFGAEKIKVGATPVPHAEILEIVKEDLAA
                     LGYELEIVVFNDYVLPNIALSTGELSANYFQHVPYLVSFTTQRGIKGLISIKAIHVEP
                     MGFFLKKPLESLEAGDKIAIPNDPTNEGRALILLHNNGFIQLKDPTKLESTVRDIETN
                     PRKLSFVEIEAGFIPRVYTDDKTVVGAVINTNYALTINLNATKDAEFIEGGESPYANI
                     ITIREADKDKDWVKALVSVLTTEKVRNFINEKYGGAVVPVF"
     gene            complement(2846..3754)
                     /locus_tag="PLD15_07655"
     CDS             complement(2846..3754)
                     /locus_tag="PLD15_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB63819.1"
                     /translation="MKSKNSSVPELVLVTGFLGSGKTTFLKVLMSQYRDVHKGVIVND
                     FGKVAIDGSLLEEGIFKEEISGGSIFCSCRENEFVEALKSMIKKGPEIIFVETSGMSD
                     PSGIERLLPIAGKVEYISAYCVVDAPRFLALSDIFSVVEKQVASSDVVIINKVDKATG
                     AELERIKQEISMIGNMPQYQARYGMTGKAIPLFKRKKTGTESENHPDNRPKSLLIEWE
                     GEVTIEQLKNFVGEILGLVLRVKGFCRTESGLVLVSGAGSSVEITLADKASETGLSVI
                     SERNKPVLKSLKETWERIFNIEMKIT"
     gene            complement(3726..5099)
                     /locus_tag="PLD15_07660"
     CDS             complement(3726..5099)
                     /locus_tag="PLD15_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB63820.1"
                     /translation="MEFIAREETGKELLLESLENKTLPRAPWVPFAGVHAGKLKGYTA
                     REVLTDGEKLVQSLLEVNRLYRPDGQPVVFDLQLEAEALGCDLVWSDVNPPSVSGHPL
                     KKDREIHGLLPDESSGRIPLVLEAMREMKRKVGRHTALFGLVCGPLTLASHLRGTDFF
                     MDIIEEPQYAQDLLSYTSDVCNRMAGMYIEAGMDVIAFVDPMVSQISPRHFKRYLSGP
                     FSGLFASVRQNGAKSSFFVCGDATKNIEAMCQTGPDSLFVDENIDLVSAKEITDSYGV
                     VIGGNIPLTTTMLYGNQKDNMKYVIELLERLGTRNYIVAPGCDMPYDVPVENGIAIQQ
                     AVRDVERVKAILENYETTEEGIEIDLPDYEHLEKPLIEVFTLDSATCAACTYMMSVAR
                     LALEEFKDRVDVVEYKFTVRENIHRIKKMGVKNLPSIYINGRLKYSSIIPDRREFFSE
                     IEKLLSS"
     gene            complement(5189..5566)
                     /locus_tag="PLD15_07665"
     CDS             complement(5189..5566)
                     /locus_tag="PLD15_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63821.1"
                     /translation="MSSREVPDRSQLFTRLDMGMVDASPIARDNGIKLPVILTEKLWD
                     EVVQTDEDSRLYGQKEEKRLASLLRTLKMELIKCRAKDLSFTFIACKGPGSTSCRLLK
                     ASYREDKSGKLYLVLSVEGEKLS"
     gene            complement(5708..6133)
                     /locus_tag="PLD15_07670"
     CDS             complement(5708..6133)
                     /locus_tag="PLD15_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPB63822.1"
                     /translation="MSATKSYRKELYFNTETRRKIVNITPQVELAVRESGVKEGLVLV
                     NAMHITASVFINDDEPGLHQDFEKWLEGLAPEKPYSQYAHNGFEDNADAHLKRTVMGR
                     EVVVAITEGELDFGPWERIFYYELDGKRKKRVLIKIIGE"
     gene            complement(6165..>6392)
                     /locus_tag="PLD15_07675"
     CDS             complement(6165..>6392)
                     /locus_tag="PLD15_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB63823.1"
                     /translation="LFKVIRHPAYAAEIVGFLGLALVFNYPLSSVLAFALPTAGIIYR
                     IFVEEKNLRQIFGKEYLLYSRRTWRLIPFVF"
BASE COUNT         1384 a         1775 c         1534 g         1699 t
ORIGIN      
        1 gggagagaac cggtctcgta gaagtcgttt tgaacatcga caaccagcag cgctttgcgc
       61 atttctatca cctcgcttca gtgttttacc gaaacgcccg gaacaaaaag agcgatcgct
      121 ttcgacggtt cgagtttctg tgtttgccct taggattcaa cgaaaccgac ccaaataatc
      181 ttctattctc gccgcgtctt tatctcctca atagtcgcga gagccttcct cccgtgaatt
      241 gcagtacctg gcttatgctc accagaacta ttacggcgta aagcaccacg tcccactgga
      301 acctgtagta accgtagttt atggccagct gtcccagacc gccggcgccg acggctcccg
      361 ctatcgcggt gtaagtcacg agattgatca gaagaacggt gatattgcct atttgctccg
      421 gcagcgtctc gggaatcaga actctgaata tgaactgcca tctgttgagt cccatggagt
      481 cggaagaatc tataatccct cgcgggactc cgttgaagga gttctccgaa agcctggcca
      541 tgaagggaat cgcggctatc gtgagattga agatagcggc gtttggccct atgatagtac
      601 ccatgacgtt tttcgtgacg ggaatcagca atagaaccag tattatgaag gggatcgagc
      661 ggaaaacgtt gactaacagg tcgaagacta tgtagagcct tcgccacttc ttggatctcg
      721 agagcaggaa gagggccact ccgaggggta ttccaaagag cgtcgcaaga aaaccggaga
      781 ccagaatcat atatagcgtc tcaaacgtgg ctttcagtat ctcgccggac atcattgaac
      841 ctcctcgagc gaatagaccg actccctgag gcgctggatg aacaggtcct gctgttcacc
      901 gtctataccg attatcatcg caccgaacgg tccatgcttc aattcttcga ttttgccgta
      961 cagtatgttg accgagacgt ccagttccct ggcaactttc cacattaccg cttcgtgcgt
     1021 cttcttaccc cagaagagga cccgtattat cttgcctctg tgttctatcg cttttctatc
     1081 cagttcgagc ggcgtgccga aatccctgac ttcgtttttt tcgactttaa cgaaaaattc
     1141 gtgggccggt ccgaagaact tcagtttccc gttttcgagg taggccactt tatcgcaggt
     1201 tctctttatc acatccatgt cgtgggtaac tacgacaaag gtgattctat gtttttggtt
     1261 gagttcgacg accagatcca gcaaccggcc ggtcgtcttc ggatcgaggg ccgaggtcgg
     1321 ttcgtccaga agtatcatct taggatccat gacgagcgcc ctggctatgg cggccctctg
     1381 ctgctctcct ccggaaagtt gagacgggta tgcctgtatc ctttgatcta ggcccagctc
     1441 gccggagatc ttttcgacct tctctttcac ttccctgctc cccagtccct gtatcttgag
     1501 gggaaaggcg atgttctgag ataccgttcg gtttctgaag agattgaagc tctggaaaac
     1561 gacgccgatc tttttgcgca tctttcttat ctcttcatct ttcaaattga ctatgttctt
     1621 tccatcgaag aatatctcgc cgctatcgac aggctggaga aggttgagcg tccggagcag
     1681 ggaggttttc cccgccccgg aaagcccgac tatgccgacg atctggcctt cttccaccga
     1741 aaagctcaca tcgtccagga tctttcttct cccggctcta tcttcgaagg aaaggttcag
     1801 gtttttgact gccaggatca aagcgaccgc tcctctcaga ataccggtac taccgctccg
     1861 ccgtacttct cgttgatgaa gtttctcact ttctccgtgg tcagcacgct cacgagcgcc
     1921 ttgacccagt ccttgtcttt gtcggcctcg cggatcgtta tgatgttggc gtacggagat
     1981 tcgccgcctt cgatgaattc tgcgtctttg gtggcgttga ggttaatcgt cagggcgtag
     2041 ttcgtgttga tcacggcgcc gaccacggtt ttgtcgtcgg tgtaaaccct tggaatgaaa
     2101 ccggcttcga tttcgacgaa actgagtttt ctcggattgg tctcgatgtc acgcacggtc
     2161 gattccagct tcgtgggatc tttcagttga ataaagccgt tgttgtggag aagtatcagc
     2221 gcgcgcccct cgttggtggg gtcgttcgga atggcgatct tgtcgcccgc ctccaggctc
     2281 tccagaggtt tcttcaggaa gaagcccatc ggttcaacgt gaatcgcttt gatggatatc
     2341 agtcccttta ttcccctctg ggtagtgaag gagaccaggt acggcacgtg ctggaagtag
     2401 ttggcgctca gctcgcccgt ggagagcgct atgttcggaa gcacgtagtc gttgaatacg
     2461 actatctcga gttcgtaccc gagagccgcc agatcctctt tcactatctc cagtatctcc
     2521 gcgtgtggaa cgggagtcgc cccgactttg atcttttccg ccccgaaggc caaaactaaa
     2581 cccagcagaa cccacaccag tagcagtttc ttcatacaaa tccctcctga tataaaaaaa
     2641 gggcgtctcc tttgagacgc cccgcttcta tttttccagg tacagctaag cacccgaaga
     2701 aacaagggcg ttgccgtaca tcatcataga tattctcttc ttcccgccga ctgctctaaa
     2761 atccataggt ttcccccaac agttcgtttg agaaattata actcacggct gctttcaaca
     2821 atgcaaacgt actgtaaaca atccctcaag tgattttcat ttcgatattg aagatcctct
     2881 cccatgtctc ttttaagctc ttcaaaaccg gtttgttccg ctcggatatg accgaaagcc
     2941 cagtttcact ggctttgtcg gccagcgtta tctccaccga cgaccccgca ccgctcacga
     3001 ggacgagacc ggactcggtt ctgcaaaaac ccttcactct caagaccaag ccgaggatct
     3061 ctcccacgaa gttcttcaat tgctcgatgg tcacctcacc ttcccattct ataaggaggg
     3121 atttcggacg gttatccggg tggttctccg attccgtccc ggtctttttc cttttgaaaa
     3181 ggggaatagc cttgccggtc attccgtacc tggcttggta ttgaggcatg tttcctatca
     3241 tggatatttc ctgttttatt ctctccagtt ccgcacccgt tgccttatct actttgttga
     3301 ttatcactac gtcggacgag gcgacctgtt tttcgacgac cgagaagatg tcgctgaggg
     3361 ccaggaacct cggggcatcg accacacagt aagcagatat atactccacc tttccggcga
     3421 tcggaaggag cctctcgata ccggacgggt cggacatgcc cgaagtctca acgaagatga
     3481 tctccggtcc ctttttgatc atggacttca gagcttccac gaattcgttc tccctgcagc
     3541 tgcagaagat cgagcctccc gagatctctt ccttgaagat tccctcttcc aggagcgatc
     3601 catcgatggc caccttcccg aagtcgttta ctatgacgcc cttatggaca tcacggtact
     3661 gcgacatcag gaccttaaga aaagtcgtct ttcccgatcc aagaaaaccc gttacaagaa
     3721 ccagctcagg aactgaggag tttttcgatt tcactgaaga attccctcct gtcaggaata
     3781 atcgatgagt acttcaaccg cccgttgata taaatggaag ggaggttctt cactcccatc
     3841 ttttttattc tatggatatt ttcgcgaacc gtgaatttgt attccacaac atcgactctg
     3901 tctttgaact cctcgagggc gagtctcgcc acgctcatca tgtaggtgca ggccgcgcag
     3961 gtggccgaat ccagtgtaaa aacctcgatc agcggctttt cgagatgttc gtaatcggga
     4021 aggtcgatct cgatcccttc ctccgtggtt tcgtagtttt cgagaatcgc tttcaccctt
     4081 tcgacgtccc ttacggcctg ctgtatggcg atgccgtttt cgacaggcac atcgtagggc
     4141 atgtcgcatc cgggagccac tatgtagttt ctcgttccca gtctctccag aagctctatc
     4201 acgtacttca tgttatcctt ctggtttccg tacagcatcg tggtcgtgag gggtatgttg
     4261 ccgccgataa ccactccgta gctgtcggtg atttccttcg cgctcaccag gtcgatgttc
     4321 tcatcgacga ataaggaatc cgggccggtc tggcacatcg cttcgatgtt cttggtggcg
     4381 tctccgcaga cgaagaagga actcttcgcc ccgttttgcc tcaccgaggc gaaaagcccg
     4441 gaaaatggtc cgctcaggta tctcttgaaa tggcgcggag agatctgcga aaccatggga
     4501 tcgacgaagg cgattacgtc catcccggcc tcgatgtaca tcccggccat gcggttgcaa
     4561 acgtcgctgg tgtatgagag caggtcctga gcgtactgcg gctcttcgat gatgtccatg
     4621 aagaagtcgg ttccgcgtag atgcgaggct agagtcaaag gaccacagac gagcccgaac
     4681 agcgcggtgt gacggcccac cttcctcttc atctctctca tggcctccag taccagagga
     4741 atcctccccg aactttcatc cgggagaagg ccgtgtattt cccggtcctt cttcaaaggg
     4801 tggccgctga ccgagggggg attgacgtcg ctccagacca gatcgcaacc cagagcttcg
     4861 gcttccagct ggaggtcgaa aacgaccggc tggccgtcag gtctgtagag tctgttgact
     4921 tcaagcagag attgaaccag cttctcgccg tcggtaagta cctctctggc ggtgtaacct
     4981 ttgagtttgc cggcatgaac gcccgcgaag ggtacccagg gcgcgcgggg taaggttttg
     5041 ttctcgagcg actccaacag cagctcttta ccggtctctt ctctcgcgat aaactccatt
     5101 gtaaacctcc cggaaaaaaa cctttctatt attctaacaa aaaagggagc tccctgaagg
     5161 gcctccctct ttatgaaact cttaagattc agctcaattt ctcgccttca acactcaaaa
     5221 cgagatacag ttttccggat ttgtcttccc tgtagctggc cttcaaaagt ctgcaactgg
     5281 ttgaaccggg ccctttacag gcgataaacg tgaacgagag atctttcgcc ctgcacttta
     5341 taagctccat tttcagagtc ctcaaaagac tggcgagcct cttctcttct ttctggccgt
     5401 atagcctcga gtcttcgtcg gtctgaacca cttcatccca gagcttctcg gtgaggatta
     5461 cgggaagctt tataccgttg tccctggcga taggtgaagc atcgaccata cccatatcca
     5521 gccgggtgaa aagctgagag cgatctggca cctctctgct gctcatacag cacctccagt
     5581 tagttgtcac tttaaacata acattcatac tgtctcgagt caaaagccga caacgaacca
     5641 caggtatgtt cagggtcgtt taggtgactt tatctgcaat agtctccggg aaactccccc
     5701 gggtgagtca ttctcctatt atttttataa gcaccctttt ctttctcttg ccatccagct
     5761 cgtagtagaa tatcctctcc cagggaccga aatccagctc cccttcggtt atggccacca
     5821 cgacctctct gcccatgacg gttcttttca ggtgggcgtc ggcgttgtct tcgaacccgt
     5881 tgtgggcgta ctgcgaatat ggcttttctg gcgcgagccc ttcgagccat ttttcgaaat
     5941 cctggtgcag tcccggttcg tcatcgttta tgaagacgct cgccgttatg tgcatggcgt
     6001 tcaccagcac gaggccctct ttcacaccgc tctctctcac ggccagttct acctgagggg
     6061 tgatgttgac gatctttctc ctggtttccg tgttgaaata cagttccttc ctgtatgact
     6121 tcgttgcact cacctccatt caaaatccgc actccaaaat tcgttcagaa cacgaagggt
     6181 atcaatctcc aggttcttcg cgagtagagc agatactcct tcccgaagat ctgtcgcagg
     6241 tttttctcct cgacgaatat tctatatatt atgcccgcag tcggtagcgc gaaagccagt
     6301 accgagctga ggggatagtt gaaaacgagt gcaaggccca gaaagcccac tatctcggcg
     6361 gcgtaggccg gatgccttat cactttgaaa ag
//
LOCUS       DAOOLY010000138         6385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14518_length_6385_cov_5.083412, whole genome shotgun sequence.
ACCESSION   DAOOLY010000138 DAOOLY010000000
VERSION     DAOOLY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14518_length_6385_cov_5.083412"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2915
                     /locus_tag="PLD15_07680"
     CDS             <1..2915
                     /locus_tag="PLD15_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPB63824.1"
                     /translation="VGSYYDYYGSLTLDKITPAAPGSKASLSFFSSSELDLWIIADFS
                     GTFSQFPYHALAGKNAVEVEIPEGYAYESFVLYLVSYKNYQMNYSWMIEVDSPLKDYS
                     VKLITEERYAPGENVNLKVEVTDPDGKPASVGLTVAVVSQAMLSLFEGDQDYWKGSLM
                     NPFYRGFGSITLVDLYYSPYPTIARLGDLLESQPPAAESKMLANEAYGMGDRGGSGEG
                     LTSDVKARKLFSDSALWSVGTFTDEAGRAELAFVVPEDLDTWTVRALASDMGGGFSYA
                     RSSFETWKPMTVSSFLPEFLIAGDKVQLVFSVKNNTDSQMPVLSGFYVDGKVIEEKGS
                     TVPAFQSSTFRYEVNVPDLEASQKDEKLTVKFVVEGSRGSDGVEYEIPVKPRFTYVRF
                     GELDFLNGERTLEFSANSVGWITVASTIDPILLEAIRYLVDYPYGCVEQTMSRFLPAV
                     AASKLLESADEAFADRVKVVTDQSLERLYGYQHYDGGWGWWRDDQTDAFMTAYVMYGF
                     YLAMENGYEINSDVIRMGYSAMKDLHSSSPDPFLQYVIALFSRKLRDPISAIVDYKSD
                     VPSMILSSLTAKVFNLNERAKSLFDEAMSYVDLESDKPIKGSSFNYFFDETVTLSFLL
                     KAAVNLQISPDAIGAISRKLLTMGDGSYWYRTASTALAVASLASFSSSLSSDAEITVT
                     ANGSPIEAGSNGTSLTFAIVPGVMTIKTTGLVAVSINGESAIPAEEVKPSESGIGLER
                     ILRRKLSVPMDNTYVLTTPQLDSPYVVASLKLLSPDEVEGLEIDVARQLEGMELAVVD
                     GELMLGSYHLGLKIYEGEIMGSTPEGFVLKTVESDYYLSGVEIYLMTLKPQSPVKAGE
                     TVISEVRVNIPENAPYIVVEDMLPSTGIAIEENPEGDMLGFSKFYYFDYWWGYTFRED
                     RFDRVAFFFRYGGEFVTRTTWRVLTTGEFLMPAVQAWAMYDGQYSANTSSILLKVE"
     gene            2912..3454
                     /locus_tag="PLD15_07685"
     CDS             2912..3454
                     /locus_tag="PLD15_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63825.1"
                     /translation="MKRVAIVSFLCASILLAGYTSSHIGWDSRELSVYRSALERLTGR
                     TVAGTYNVIIASSAGEFSRLSRLGYWFVAATHGDTIYLQPLSLIPDLARTLAHEMSHI
                     FFQRFDLPYWLEEGLVCTITGEWVGREEPLLENIEELDHTGMDFMTYRAYSFSCWRRV
                     GQLLREHGFNELISRFVAAQ"
     gene            3536..4075
                     /locus_tag="PLD15_07690"
     CDS             3536..4075
                     /locus_tag="PLD15_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HPB63826.1"
                     /translation="MDLGGECLKRWELAGFFFIFFAGALLHFAYELSGENVFVGALTP
                     VNESVWEHLKLVFLPGVILFFMEIGFCKGISGSSLLAGKTLGIYIMCGTILGGFYLYT
                     LFVRHNLIIDLLLMAFAIYLGQAVSFKIASAGKATVATAVVATIFLIALGAIFVVFTF
                     RPPELEPFRDSLTGKYGTG"
     gene            4139..4498
                     /locus_tag="PLD15_07695"
     CDS             4139..4498
                     /locus_tag="PLD15_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HPB63827.1"
                     /translation="MSGLLRYLIKIMISAIIITVVSEVSKKTGYIGGLIASLPLTSML
                     ALFWLYNDTKDVDKVAGLSTSILLFVIPSLIFFVVMPLLLKKDMNFYLALAISSGAMV
                     SGYAFFSFILSRFGIKL"
     gene            4627..>6385
                     /locus_tag="PLD15_07700"
     CDS             4627..>6385
                     /locus_tag="PLD15_07700"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPB63828.1"
                     /translation="MSLNSTIGEAMTIKLDDRLPPEPVFEPSIRRAPKRELNLGGAEI
                     ELALKNALRYIPGELHERLAPEFLRELLTRGRIYGYRYRPQGRIKGKPIEEYRGNCIE
                     GRAFQVMMDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLIKRYLEELTDEQTLVVM
                     SGHPLGLFKSHRHSPRVVITNALMVGMFDNQREWIRAQALGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRMKLGIPDAGDLKGHLFVSSGLGGMSGAQGKAVRIAGGVGIIA
                     EVDISRIKTRLEQGWIDSLARSPEESFRIAAEKLASGESHAVAFHGNIVDLLQHAVDE
                     KIPVELLSDQTSCHAPYDGGYCPQGLSFEERTEMLASDKERFRRLVDDSLRKHFGLIK
                     TLVERGTYFFDYGNSFMKAVFDAGAKEIARNGRDTSEGFIFPSYVEDIMGPMLFDYGY
                     GPFRWVCLSGLREDLLKTDRAAMECIDPDRRGQDRDNYIWIRDADRNGLVVGTQARIL
                     YQDAEGRKKIALKFNEMVRKGEIGPVMLGRDHHDTGGTDSPFRETSNIKDGSNVMADM
                     ATQCFAGNAARGMSLVALHN"
BASE COUNT         1452 a         1693 c         1835 g         1405 t
ORIGIN      
        1 acgtaggctc ttactacgac tactacgggt ctctgacgct ggacaagata acaccggccg
       61 ctcccggttc gaaagcgagc ctctccttct tctcctccag cgagctcgac ctctggatta
      121 tcgccgattt ctccggcaca ttctcccagt tcccttatca cgcgctcgcc ggcaaaaacg
      181 cggttgaggt cgaaataccc gaaggctacg cctacgagag tttcgtgctg tatctggtca
      241 gttacaagaa ctaccagatg aattacagct ggatgataga ggtcgattct cccctgaagg
      301 actacagtgt gaagttgatc accgaagaga gatacgcccc cggagagaac gttaacctga
      361 aggtcgaggt aaccgatccc gacggtaaac cggccagcgt cggattgacg gtagcggtcg
      421 tctcgcaggc catgctctcg ctcttcgaag gagaccagga ttactggaag ggttcgctca
      481 tgaatccctt ctacagaggt ttcggctcga tcactctggt agatctctac tactccccgt
      541 atccgaccat agcgcgactg ggcgacctcc tcgaatcgca gcccccggcg gccgaatcga
      601 agatgctggc caacgaggcc tacggaatgg gagatcgcgg cggttcggga gaaggtctca
      661 catcggacgt gaaagcaaga aaactcttca gcgacagcgc cctctggtcg gtcggaacct
      721 tcaccgatga ggctggccgg gcggaactcg cgttcgtagt tcccgaagat ctcgacacct
      781 ggaccgtgag agctctggcg tccgatatgg gcggagggtt cagctacgcc agatccagct
      841 tcgagacgtg gaagccgatg actgtcagca gcttccttcc cgagttcctg atagccggcg
      901 acaaggtgca gctcgtcttc tcggtcaaga acaacaccga ctcgcagatg ccggttcttt
      961 cgggtttcta cgttgatgga aaggttatcg aggagaaggg ttcgacggtg ccggccttcc
     1021 agagctcgac tttcagatac gaggtgaatg tacccgatct cgaagcctca cagaaagacg
     1081 agaaactcac ggtgaagttc gtcgttgaag ggagcagagg ctcggacggc gtggaatacg
     1141 aaatacccgt gaagccgagg ttcacttacg tgaggttcgg cgaactggac ttcctgaacg
     1201 gcgagagaac gcttgaattc tccgccaact ctgtcggctg gataaccgtc gcctccacga
     1261 tagatcccat acttctggag gccatacgtt acctggtaga ctatccgtac ggttgcgtag
     1321 agcagaccat gagccgtttc ctgcccgccg tcgccgcttc gaagcttctc gaaagcgccg
     1381 atgaagcctt cgccgataga gtgaaggtcg taacggacca gagtctggag agactgtacg
     1441 gctaccagca ctacgatgga ggctggggct ggtggagaga cgaccagacc gacgccttca
     1501 tgacggctta cgttatgtac ggtttctatc tggccatgga aaacggctac gaaataaaca
     1561 gcgatgtcat caggatgggt tactcggcca tgaaggatct gcacagttcc tctcccgacc
     1621 cattcctgca gtacgtgata gccctcttca gcagaaagct cagagatccg ataagcgcca
     1681 tcgtcgatta caagagcgat gtcccctcca tgatcctttc ttcgctcacg gccaaggtgt
     1741 tcaacctcaa cgaaagggcg aaaagcctct tcgacgaggc catgagctac gtggatctgg
     1801 aaagtgataa accgatcaag ggcagcagct tcaattactt cttcgacgag accgtgacgc
     1861 tctccttcct gttgaaggcc gccgtcaatc tccagatttc gcccgacgcg atcggagcca
     1921 tttccagaaa gcttctcacg atgggtgatg gcagttactg gtacagaacg gcttcgacgg
     1981 cgctggcggt agcctcgctg gcctctttca gctcttctct atccagcgat gcggagatta
     2041 ccgttacggc aaatggttcg cctattgaag ccggaagcaa cggtacctcc ctgacattcg
     2101 ccatcgtgcc cggagtcatg acgatcaaaa ccaccggact cgtcgccgtt tcgatcaacg
     2161 gagagagtgc gattccggcc gaagaggtaa aaccctccga gagcggaatc gggctggaaa
     2221 ggatactgag aaggaagttg agcgtcccga tggataacac ttatgttctc acgaccccac
     2281 agctcgattc gccgtacgtt gtggcttccc tcaagctgct ctctcccgac gaagtcgaag
     2341 gactcgagat agacgtcgcc aggcagctgg aaggcatgga gctggccgtc gtagatggtg
     2401 aactcatgct cggttcgtac cacctcggac tgaagatata cgagggcgag atcatgggca
     2461 gcacccccga aggcttcgtg ttgaagacgg tcgaatccga ttactatctc agcggcgtgg
     2521 agatatatct catgaccctt aagcctcagt cgccagtgaa ggccggagag accgttattt
     2581 cggaggtgcg cgtcaacata ccggaaaacg cgccttacat agtcgtggaa gatatgttgc
     2641 cctcgacggg gatcgcgatc gaggagaatc ccgagggaga catgctgggc ttctcgaagt
     2701 tctattactt cgattactgg tggggctaca ccttccgtga agacaggttc gacagggtgg
     2761 ccttcttctt caggtacggc ggcgaattcg tgaccagaac gacctggaga gtgctcacga
     2821 ccggcgagtt cctcatgccg gccgtgcagg cctgggccat gtacgacggt cagtactcgg
     2881 ccaacacgtc gtcgatcctt ttgaaagtgg aatgaagaga gtcgcgatcg tttcattcct
     2941 ttgtgcatcg atccttctgg caggctacac cagctcccac atcgggtggg actcccggga
     3001 gctgtcggtt tacaggagcg ctctggagag actgacgggc agaacggtcg cgggcaccta
     3061 caacgtaatt atcgcttctt cggcgggaga gttttcaagg ctctcccgcc ttggttactg
     3121 gttcgtggcg gccacacacg gagacaccat atatctccag ccgctcagcc tgattcccga
     3181 cctggcccgg acactggctc acgagatgtc gcacatcttt tttcaacgct ttgacctccc
     3241 ctactggctg gaggagggtc tcgtttgcac aatcacgggc gaatgggtcg gcagggaaga
     3301 gccgttgctg gaaaacatcg aagagctgga tcacaccggg atggatttca tgacttacag
     3361 ggcctacagt ttctcctgct ggagaagggt gggccaattg ctcagggagc acggcttcaa
     3421 cgaactgatc tcgagattcg tggccgctca gtgaagccgg tccactccga cctgccatac
     3481 agggagtcgt ctcgcggttt tccggagtct tcgcgataga tagagatccg gggtgatgga
     3541 tttgggtggc gagtgtttga agcgatggga actggcgggt ttctttttca tcttcttcgc
     3601 gggcgcactg ctccacttcg cgtatgaact gagcggagag aacgttttcg tcggagcgct
     3661 taccccggtg aacgagagcg tctgggagca cctcaagctc gtttttctac cgggggttat
     3721 tctctttttt atggaaatcg gattctgtaa aggcatttcc gggagctcct tgctggccgg
     3781 caaaacactg ggaatatata taatgtgcgg cactatcctg ggcggctttt acctatatac
     3841 gctgttcgtc cgccacaatc tgatcatcga tcttctcctg atggccttcg ccatctatct
     3901 gggacaggca gtctctttca aaatcgcctc ggccggaaaa gcaaccgtgg cgactgccgt
     3961 tgtagccacc atttttttga tagctcttgg agcgatcttt gtagttttca ccttccggcc
     4021 gcccgaactg gagccgttca gagattccct gacggggaaa tacggtacag gatgaggcgg
     4081 tgacggttct taagatcatt tgagaagttt tcgaaaaaca ggcagaataa atcgcggggt
     4141 gagtgggttg ctgagatact taattaaaat catgatatcg gcgataataa taaccgttgt
     4201 ttcagaggtc agcaaaaaga ccggctacat aggcggactt atagcctcat tgcctctcac
     4261 atcgatgctg gcgcttttct ggctctacaa cgacacgaag gacgtcgaca aagttgccgg
     4321 gctctccacc agcattcttt tgtttgtgat tccatccttg attttctttg ttgttatgcc
     4381 actgctcctg aagaaagata tgaatttcta cttggcgcta gcgatttcaa gcggggcaat
     4441 ggtgtcaggg tacgcgtttt tttcgttcat cctttccagg ttcggtatca agttgtgatt
     4501 ttcacacgaa acgttgcggt ttcctgatcg ctatcttttc cctttcaaat agcgctcgat
     4561 gtccactttt tcattggtag ttggataatg atagaattgt cactataaag gaaagggggg
     4621 gtttgcgtgt ctcttaattc caccattgga gaggcgatga cgataaagct tgacgatcgt
     4681 ctgccaccgg aaccggtctt cgagccgtcc ataaggaggg ctccgaagag agagttgaat
     4741 ctcggcgggg ccgaaatcga actggccctc aagaacgctc tcaggtacat ccccggtgaa
     4801 ctccacgaaa gactggcgcc ggaattcctg cgggagcttc ttacccgggg cagaatttat
     4861 ggttacagat acagaccgca gggcaggata aaagggaaac ccatagagga atacaggggt
     4921 aactgcatag aggggagggc cttccaggtg atgatggaca acaacctgga cttcgatgtc
     4981 gcgctctacc cgtatgaact cgtcacttac ggcgaaaccg gccaggtctg ccagaactgg
     5041 atgcagtaca ggcttataaa gagatacctg gaggagctga ccgacgagca gacgctggtg
     5101 gtcatgtcgg gccacccgct gggtctcttc aagtctcaca ggcacagccc gagggtcgtt
     5161 atcaccaacg cccttatggt cgggatgttc gacaatcagc gggagtggat acgtgctcag
     5221 gcgctcggtg tggccaacta cggacagatg acggccggcg gttggatgta cataggccct
     5281 caggggatcg tccacggcac tttcaacacc attctcaacg cggggagaat gaagctgggc
     5341 atacccgacg ccggcgacct gaagggtcac ctcttcgtgt cgtcgggtct gggcggcatg
     5401 agcggcgcgc agggaaaggc ggtgcgtatc gccggaggag tcggcatcat agcggaggtc
     5461 gatatctcca ggataaagac gcgtctcgaa cagggctgga tcgattctct cgcccggagt
     5521 ccggaagagt ctttcaggat cgcagccgaa aagctcgcct cgggcgagag ccatgcggtg
     5581 gccttccacg gaaacatcgt ggatctcctt cagcacgcgg tggacgagaa gatacccgta
     5641 gagctgctct cggaccagac ttcctgtcac gctccgtacg acggtggcta ctgtccgcag
     5701 ggcctgagtt tcgaggagcg gacggagatg ctggcctccg acaaagagag gttcaggaga
     5761 cttgtggatg attctctcag aaaacacttc ggcctcataa agacgctggt ggagaggggg
     5821 acctatttct tcgattacgg caactccttt atgaaggccg tcttcgacgc gggtgcaaag
     5881 gagattgcca gaaacggccg cgacacttcc gaaggcttca tcttcccctc ttacgtcgag
     5941 gatataatgg ggcctatgct cttcgattac gggtacggtc ctttcagatg ggtctgcctg
     6001 agcggcctga gggaagacct tctcaagacc gacagggcgg ccatggagtg catagacccc
     6061 gacaggcgcg gtcaggacag ggacaactac atctggataa gggacgccga cagaaacggg
     6121 ctggtggtcg gaacacaggc caggattctc taccaggacg ccgaaggccg gaaaaaaata
     6181 gcgctcaagt tcaacgaaat ggtcagaaag ggagagatag gaccggtgat gctgggcagg
     6241 gaccatcacg ataccggagg aaccgactca cccttcagag agacctccaa tataaaggat
     6301 ggaagcaacg tgatggccga catggcgact cagtgtttcg ccggaaacgc cgccaggggt
     6361 atgagtctgg tcgccctcca caacg
//
LOCUS       DAOOLY010000139         6330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14653_length_6330_cov_5.133227, whole genome shotgun sequence.
ACCESSION   DAOOLY010000139 DAOOLY010000000
VERSION     DAOOLY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14653_length_6330_cov_5.133227"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLD15_07705"
     CDS             <1..739
                     /locus_tag="PLD15_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB63829.1"
                     /translation="QCKTGRMHICRDMEILGVHRDGVFAEYVLVPEIVLWKVDPSIPL
                     EYASIMEPFGNAIHTALVTDLTGKNVLITGAGPIGAMAVAVAKASGAACVMVSEVKDF
                     RKDLAKKMGADVVIDPLKEDMPTRVRSLTNGDGADVLLEMSGSPIAMKQGLDSVTNGG
                     IVSLLGVFPGDLAFDINNLFTFKGLTMYGITGRKMFDTWRIADQLLKSKRVDLSPVVT
                     HTFPAERFEEGFDLMEKGLSGKVILQF"
     gene            768..1952
                     /locus_tag="PLD15_07710"
     CDS             768..1952
                     /locus_tag="PLD15_07710"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPB63830.1"
                     /translation="MFNFDVLKDEMRELEEKGLLVKIRTLESPQGAWLNVDGRKVLNM
                     CSNNYLGLCFDEELKQAAIEGIKKWGVGPGAVRSIAGTLELHNELERELAQFKKVEAT
                     LVVQSGFNANQAVIGPIVSEEDAILSDELNHASIIDGVRLTKAKRYVWKHKDVADLEE
                     QLKAAKADGARRMLIITDGVFSMDGDLAPLPEIVEKAEKYGAIVMVDDAHGEGVVGSN
                     GRGIVDHFHLHGRVDIEVGTLSKAFGIVGGFIAGKKALIDYLKQKARPFLFSSSLSPA
                     ETAAATAAVRKLSSSDELVQTLWSNAGYFKERLKSLGFDTGHSETPITPVMLYDAKLS
                     SNFSKRLFEEGIFASSIGFPTVPRGKARLRIMISAAHSRKDLDFAISKFEMIGRELGV
                     IK"
     gene            2036..2995
                     /locus_tag="PLD15_07715"
     CDS             2036..2995
                     /locus_tag="PLD15_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPB63831.1"
                     /translation="MIWTLVLLGIGFLLLIKGADYLIEGAVAIAKRFGLSELLIGLTI
                     VAFGTSAPELAVSFQAALKGSGIALGNVLGSNIANIALILGAAALISPLKVSNSTMYY
                     EIPFIIIISAASGAMFFGRNFTLGRSDGIVLLAFFVIFMMYIFSMARQDRAISQAVQI
                     QEKKDTISFGRDIKWAWIATIGGLVAVIIGGDLVVDAGSHIARSFGVSDMLIGVTIVA
                     IGTSLPELVTSITAGRKGRNDLAVGNVIGSNIFNTLFILGASALASPLKADRPFMMDI
                     AFNVGLSIALPLLIMKRRVLSKFSGLLLVALYTVYLLLNTIAG"
     gene            3097..5091
                     /gene="uvrB"
                     /locus_tag="PLD15_07720"
     CDS             3097..5091
                     /gene="uvrB"
                     /locus_tag="PLD15_07720"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPB63832.1"
                     /translation="MKFELDSTYKPQGDQPQAIEKLTEGLSKGDRFQTLLGVTGSGKT
                     FTMANVISNLQRPAIVISPNKTLVAQLYREFKTFFPASRVELFISYYDYYMPESYIPS
                     KDMYIEKEAEINETLERMRVSTLKSVLTRNDTVVLASVSAIYASGDPRDFATMNIHLQ
                     KGSRFNRNELAIRLANIQYNRSEDLSAGGVFHMKGEIFEIFPPYEDFGIRICFFDDEI
                     ERIVTFEPLNRKVMDELDMVTIYPAKEFVTTQDKILGAMKSIEEELEERVKELEREGK
                     YLEAQRLRQRTTYDMEMLSSVGYCSGIENYARFFDGRRRGEKPYTLLDYFDREKFITF
                     IDESHIGVPQIRAMYRGDFSRKKNLVDYGFRLPAAFDNRPLKFEEFLETVGQIVFVSA
                     TPSEYELGLSRQVVDQVIRPTGLVDPEVIVQPTDSQVDDFIFRMKPVRKRNERALVTV
                     LTKKAAEMLSDYLNEVGIKSEYLHSELDAIERVEVLRKLREGTIDVVVGVNLLREGLD
                     LPEVSLVAIMDADKEGFLRSEITLVQTIGRAARNINGQVVLYADTMTGSMQRAINETN
                     RRRLKQLIYNKEHGIIPESIVKPLYRNIFEEFAGENLDKDREDKARTTYIEGVFALKE
                     NLDTEDYMALLNEEMIRAAGELRFEDAAVIRDEMYRLKKK"
     gene            5336..6103
                     /locus_tag="PLD15_07725"
     CDS             5336..6103
                     /locus_tag="PLD15_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB63833.1"
                     /translation="MKKVMLVVFLVVVSLAFSSLLEDIRSRGVLRVGQDAGYMPLYGT
                     DPDGKRIGLEVEILKEMASILGVKLEFVIVNWDGIIPALVSNKFDIIWSGMTITPERA
                     LKVNFSDPYLTVGQTILYNTKKFSTPPTLEDINTKETKIAVQLGTTGAEAANRLLTKA
                     QIFTFETTDEAGFQVASGRADAMIFDSIYAKFVAKKYEQLDVTDELLTMENLGVAIPK
                     GDFETLQWLNTFIQWLKTTEKITELEQYWFVEYAPEF"
     gene            6113..>6330
                     /locus_tag="PLD15_07730"
     CDS             6113..>6330
                     /locus_tag="PLD15_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB63834.1"
                     /translation="MKRAVSRITLGLFVAGLIWFSYFSVSQAYPFNWKVLPRYYKLFL
                     DGLVTTLELSVIALGFAIVVGIVVALART"
BASE COUNT         1687 a         1469 c         1737 g         1437 t
ORIGIN      
        1 tcagtgcaag accggtagaa tgcatatttg cagagatatg gagatactcg gcgtacatcg
       61 agacggggtc tttgccgaat acgttctggt tccggagata gttctctgga aagtcgatcc
      121 gtcgatcccg ctggagtacg cctcgataat ggaaccgttc ggtaacgcaa tacacacggc
      181 cctggtaacc gatctaaccg gtaaaaacgt tctcataacc ggagccggcc ctataggagc
      241 catggccgtg gccgtggcga aagcatcggg ggccgcctgt gttatggtga gcgaagtgaa
      301 ggatttcaga aaagatctgg caaaaaagat gggcgccgac gtcgtaatcg acccgctcaa
      361 ggaagacatg ccaaccagag tgagatcctt gaccaacggt gatggagccg atgttcttct
      421 agagatgtcg ggaagcccca tcgccatgaa acagggtcta gacagcgtca ccaacggtgg
      481 aatagtatcg ctgctgggag tatttcccgg agatctggcc ttcgatataa acaatctttt
      541 cacgtttaaa gggcttacca tgtacggaat aaccggtaga aagatgttcg atacctggcg
      601 gatagcggat cagcttctga aaagcaagag ggtggatctt tcaccggtgg tgactcatac
      661 attccctgcc gagcgtttcg aggaaggttt cgacctgatg gaaaaagggc tctccggcaa
      721 ggtaatactc cagttctaaa ccaacgttga aacgggaggt gtgatggatg ttcaatttcg
      781 atgttttgaa agacgagatg agggaactcg aagaaaaggg actgctagtg aaaattcgaa
      841 ctctcgagtc tccgcagggt gcctggctga acgtggatgg cagaaaagtg ctcaacatgt
      901 gttccaacaa ttacctggga ctttgcttcg acgaggagtt gaaacaggct gcgatagaag
      961 gtataaaaaa gtggggcgtg ggtccgggtg cggttagatc catcgcgggt actcttgaac
     1021 tccacaacga actcgagagg gagctggcgc aattcaagaa agtggaggcg acactggttg
     1081 tgcagtcggg tttcaacgcc aatcaggccg tgataggccc gatagtctcc gaagaagacg
     1141 ccattctctc cgacgagctg aaccacgcaa gtattataga cggcgtaaga ttgacgaaag
     1201 ccaaaaggta tgtttggaag cacaaggatg tcgccgatct cgaagaacaa ctgaaagcgg
     1261 ccaaggccga tggcgccagg agaatgctta tcatcaccga cggcgttttc tcaatggacg
     1321 gcgacctggc acccctgcct gagatagttg aaaaggccga aaagtacggc gcgatagtga
     1381 tggtggacga tgcgcacggt gaaggtgttg taggaagcaa cggacgcggc atcgtggatc
     1441 acttccatct ccacgggagg gtggatatcg aagttggaac tctttccaaa gccttcggta
     1501 tcgtaggcgg ttttatcgcg ggcaagaaag ccctgataga ttatctgaag caaaaggcac
     1561 gccccttcct gttcagttct tctctctctc cggccgaaac cgcggccgcc acagcggcgg
     1621 tgcgaaagct ctcctcgtcg gatgaactcg ttcaaaccct gtggagcaac gccggatact
     1681 tcaaagagag gttgaagtcc ctcggtttcg atacgggaca cagcgaaacc cctatcactc
     1741 cggtgatgct ttacgacgca aagctctcat cgaatttttc gaagcgactc ttcgaagagg
     1801 gcatctttgc cagctcgata ggcttcccga cggtgccgag gggaaaggcg agattgagaa
     1861 taatgattag cgcagctcat tcgagaaagg atctcgattt tgccatctcg aagtttgaga
     1921 tgataggcag ggagctgggt gtaataaaat aatcacttcc atttagcgag attccgatta
     1981 taatagtaaa caatgcagcg gccttgagcc gctgttttgt cgggaggtat agaaattgat
     2041 atggactctg gttctactcg ggatcggttt ccttctactt ataaaggggg ccgattatct
     2101 aatagaagga gccgtagcca tagcgaaacg tttcggcctt tccgagttgt tgataggact
     2161 cacaatagtg gctttcggaa ctagcgctcc ggagctggcg gtaagctttc aagccgcgct
     2221 gaaaggctcg ggaatagcct taggaaacgt gctgggttcg aatatcgcaa acattgctct
     2281 cattctcggt gctgcggccc tgataagtcc tttgaaggtc tcaaactcga ccatgtatta
     2341 cgaaattccc ttcataataa tcatatcggc cgcatcgggc gccatgtttt tcggaagaaa
     2401 tttcactctc ggcaggagcg atgggatagt cttgctcgcc ttttttgtga tcttcatgat
     2461 gtacatattt tcgatggcca gacaggatag ggcgataagc caggcagtac aaatacagga
     2521 aaagaaagac acaataagct tcggaagaga tataaaatgg gcctggatag ccacaattgg
     2581 cggtttggtg gctgttataa tcggaggcga cctggttgtc gatgccggtt cacacatcgc
     2641 ccggtctttc ggtgtgagcg atatgttgat cggtgtgacc atcgttgcta tcggcacatc
     2701 tctaccggag ttggtgactt ctataacggc aggaagaaaa ggcaggaacg atcttgcagt
     2761 tggcaacgtg ataggttcga acattttcaa cactcttttc atactcggtg cctctgcttt
     2821 ggcctctccg cttaaagcgg accgcccttt tatgatggat atagcgttca acgttggcct
     2881 ttcgattgcc cttcccttgt tgatcatgaa gaggagagtg ctgagcaagt tctctggctt
     2941 acttcttgtt gcgctttaca cagtttacct gttgctcaat acgatcgcgg gttgaagtat
     3001 agatattcga taacgggtgg tatataatat tatactgcaa cgacttcgca agcgtatttt
     3061 gcctgccagt tcgaagattg aaagggggca tttcgattga agttcgaact cgattcgaca
     3121 tacaaacctc agggagatca accgcaagcc atagaaaagc tcacggaggg actttcaaag
     3181 ggggacaggt ttcaaaccct tctgggggtg accggctcag gcaagacctt caccatggcc
     3241 aacgtgattt cgaacctcca gcggccggcg atagtgatct ctccgaacaa gactctagtc
     3301 gcacagctat accgggagtt caaaacgttt tttcccgcca gcagggtcga gctgttcata
     3361 agttactacg attactacat gcccgaatcg tacatacctt cgaaggatat gtacatcgag
     3421 aaggaggcag agataaacga aaccctggag agaatgcgcg tatctaccct gaagtcggta
     3481 ctgacacgca acgatacggt cgttctggca agtgtttcgg ccatctacgc cagcggagat
     3541 ccgagagact tcgcgacgat gaacatacac ctccagaaag gctccagatt caacaggaac
     3601 gagctggcca taaggctggc aaacatacag tacaaccgat cggaagatct ttcggccgga
     3661 ggggtttttc acatgaaagg agagatcttc gagatcttcc cgccctacga ggatttcggc
     3721 ataaggatct gcttcttcga cgacgagatc gaacgcatag tcactttcga accgctcaac
     3781 cgcaaggtga tggatgagct tgacatggtt accatatatc cggccaaaga gttcgtaacg
     3841 acccaggaca agatccttgg cgcgatgaag agtatcgagg aggaactgga agagcgcgtg
     3901 aaagaactgg aaagagaggg aaagtacctg gaggcccagc gtctccggca gcggaccacc
     3961 tacgacatgg agatgctctc cagcgttggt tattgctcgg ggatcgagaa ttacgcccgc
     4021 ttcttcgacg gcagaaggag aggtgaaaaa ccctatacgt tgcttgacta tttcgacagg
     4081 gagaagttca taaccttcat agacgagtcc cacataggag tgccgcagat cagggcaatg
     4141 tacaggggcg atttctcgag gaaaaagaac ctcgtcgatt acggttttcg actgcccgcc
     4201 gctttcgaca acagacccct caagttcgag gagttcctcg agacggtcgg ccagatagtt
     4261 ttcgtatcgg ccactccctc cgagtacgaa ctgggactct ccaggcaggt tgtcgatcag
     4321 gtgataagac ccaccggtct ggtcgatcca gaagtgatcg tgcagcctac cgacagccag
     4381 gtagatgatt tcatattcag aatgaagccg gtgaggaagc ggaacgaaag agcgctcgtg
     4441 acggttctta ccaagaaggc cgccgaaatg ctctcggact atctgaacga agtcggtatt
     4501 aagtcggagt atctccactc tgagctcgac gccatagaga gagtggaggt tttgagaaaa
     4561 ctgcgcgagg gcacgatcga cgtcgtggtg ggtgtcaatc tcctcagaga ggggctggat
     4621 ttgcctgaag tctctctcgt ggcgataatg gatgccgaca aagagggctt ccttagatcg
     4681 gaaatcaccc tggtgcagac cataggaagg gcggcaagaa acataaacgg ccaggtggtt
     4741 ctctacgccg acaccatgac cggctcgatg caaagggcga taaacgagac gaacaggaga
     4801 aggctcaagc agctcatata caacaaggaa cacgggatca tccctgaaag catcgtcaaa
     4861 cccctttaca gaaacatctt cgaagagttt gccggagaaa acctcgacaa ggatagagaa
     4921 gataaagcca gaaccacata catagagggg gtctttgcgc tcaaggagaa cctcgacacc
     4981 gaggattaca tggcgctcct caacgaagag atgataaggg ccgccggaga gctgcggttc
     5041 gaagatgcgg ccgtaataag agacgagatg tatcgcctga aaaagaaata actttagttc
     5101 agagaaacgt aacttgacat acgataccgg atgctgttag tatcttctca acgaaaacac
     5161 gacggaaggt gaagcgatgt acgaatacac tgtgtcctta aaaaccgaat catccgctct
     5221 tcatcatcat aaactgtggc gcccggtagt gggaggcggc accgtttagt cgtgctctca
     5281 taaaaaagat aaccaaccgg gcttaaagcc cggtttttta tttggaggtg ttggtatgaa
     5341 gaaagttatg ctggtggttt ttctggtcgt tgtgagtctc gcgttctcca gtttactgga
     5401 ggacataagg agcaggggag tactaagagt cggacaggac gccggttata tgccgctcta
     5461 cggtacagat cccgatggaa aacgtatagg gctggaagtg gagatactca aggaaatggc
     5521 ctcgattctc ggtgtcaaac tcgagttcgt tattgttaac tgggacggta tcattcctgc
     5581 gctggtatcc aacaaattcg acatcatatg gtcgggaatg accatcaccc cggaaagagc
     5641 gctgaaagtc aacttcagcg atccttatct cactgtgggg cagacaattc tctacaacac
     5701 gaaaaaattc tccacaccgc cgacgctgga ggatataaac actaaagaaa cgaagatagc
     5761 cgttcagctc ggtaccaccg gtgccgaggc cgccaacaga ttgttgacca aagcacagat
     5821 cttcactttc gagacaaccg atgaggccgg cttccaggtc gcctcgggca gggccgacgc
     5881 catgatcttc gattcgatct acgccaaatt cgtagccaag aagtacgaac agctcgacgt
     5941 caccgatgaa ctgttaacca tggaaaactt gggggtcgcc attccaaagg gtgactttga
     6001 gacactccag tggctgaaca cgtttataca gtggctaaag acgacggaaa agataacaga
     6061 actcgaacag tactggttcg tggaatacgc cccggaattc tgaggctagg cgatgaagag
     6121 agcggtttca cgtataacgc tgggtttgtt tgtggccgga ttgatctggt tctcttattt
     6181 ctcggtttca caggcatatc catttaactg gaaagtgctg cccagatatt acaagttgtt
     6241 cttggatggg ctggtcacga ccctcgaatt gagtgtaatc gctctcggtt tcgctattgt
     6301 ggttggaatt gtcgtggcgc tggcgagaac
//
LOCUS       DAOOLY010000140         6267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14836_length_6267_cov_4.317933, whole genome shotgun sequence.
ACCESSION   DAOOLY010000140 DAOOLY010000000
VERSION     DAOOLY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14836_length_6267_cov_4.317933"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLD15_07735"
     CDS             <1..612
                     /locus_tag="PLD15_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB63835.1"
                     /translation="LQMELSHEGYSVEVITEGETALKRLEKKLPDLLILDVMLPGANG
                     FRVLNKVREYISTDLPIIMLTARGDVEDRVRGLKGGADDYIPKPFVIEELLARMEAIF
                     RRRGILDRVSFHEISIDNNTKEVTVGGEPVQLSKTEFELLFALLTNAGIVMSKERLLE
                     KVWGSEEWGNPNVVEVYINYLRKKLGQSGERIKTVRGSGYVVR"
     gene            602..1876
                     /locus_tag="PLD15_07740"
     CDS             602..1876
                     /locus_tag="PLD15_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB63836.1"
                     /translation="MSDKRLFSTSTQVAFNSVVLLVVILSVSALFIYRVSESVFIRNY
                     SEEMRKIFMDSPMGRGMRMGIGRIIGRGGTEFYVRIEGIVVQNPNGLSNVPDVEGYMK
                     TRIGGSSYLFYGFTNGNDKILLGAQMDDLDFFLQSLSTTLVLSTIIGVALSILSGYLL
                     GRRVSKPLREATSLLKEITLEDLSRRVEIDPKTSELAELKKALNTALDRIEDGYKRQE
                     QFSSDIAHEIRSPLTSILGFSRMIQRWGSKDPDALKEAAESITATAGKMLTITEGLLF
                     LARPVIETNRESFNLGELVNELVQSTPVPGNITVEVDVENVQVTTDRALLKIALKVLL
                     ENAMKYGKGRPIVFYWADGSLHVRDHGDGINEGEIDRIFDRFYRSDSSRSGGGHGLGL
                     SIVRKICDTLDLTITAENNEDGGAVFSIGRLL"
     gene            1873..3384
                     /locus_tag="PLD15_07745"
     CDS             1873..3384
                     /locus_tag="PLD15_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB63837.1"
                     /translation="MKTLSYIAESILLWGVLPLWILSGSASGIGFLAVAGLVTAVVSA
                     AFSLYSTLVAFYWTGKFPGLLTVQNQTVTSGPYRFIRQPLYVGYSLFLLGVVLLSGKY
                     LFLVLWAGICCCLLLYTLFIEKKLVDRDERYAKYRETVPLLLPGRGKYIPFDFTRCVP
                     WLFIATSLVVKFLVLVLLPSKVKNARVLKERKPFIIALAHQTHYDGPLLFYSTWRYIR
                     FVGTAIYVDRMKLLRNFGTIPVKRYTIDTSAIRQMLSTIKEGIPLGIAPEAARSWDGK
                     FLSVKKEIWKLFKMLKTPIIPVKFYGIQRLWPRWAGIFSPGFSTVEFGDPVQPDDPEM
                     ERKISDFLSKEDPTFDKPYRSYRHIERLIWRCPSCGKVGSIESFRRGFSCNSCGKSWN
                     RPSVNEVIELHEKIKPGNMGLSFPVKDRVFFRGEEVDGLIMEDSAMIGDFSLKYSDIK
                     NSSIEKSIEPVFGTADEMVIFKSNSSALMWQEIIDFQIKFRLKRGDYHTDLWG"
     gene            3574..4059
                     /locus_tag="PLD15_07750"
     CDS             3574..4059
                     /locus_tag="PLD15_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63838.1"
                     /translation="MRWYGSAKGVSLVETVMNMLLTAIVISIVMVMLSITFKIGFQIH
                     SQLTIDEEIVKLSSNLKYIVSRNWTGLDLSEGLDGDTITLESRFPLIPEASVATITYL
                     DGRLMFIYRESENTLARTVLAEHLDQFTFYDLEGQYLYYRAVFEYRGATKEIDGAVRI
                     Y"
     gene            4091..4435
                     /locus_tag="PLD15_07755"
     CDS             4091..4435
                     /locus_tag="PLD15_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63839.1"
                     /translation="MLAVLAMLISSGIALILQYRSMSATLEISTNLHSAKLLVEGIVR
                     SANRVSEENIIDRIEQLSNYPGFQDVEVASVEATNIEDSPTRRIFEVVLRDRRVGVEE
                     VFHVFRFDPFAE"
     gene            4404..6032
                     /locus_tag="PLD15_07760"
     CDS             4404..6032
                     /locus_tag="PLD15_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63840.1"
                     /translation="MYSDSIPSRNRGFVLALTLILMTFATTMIIAASIIIQRTSRRLN
                     EYSLLSDLRVASNNLVEGAAMVLAEKWNQDNFNSAWEGFEEFLQFVSSRGGYEGDLWS
                     EVIQTLDSEGWWLINGDEDFASLLEDASFSELTSIGAAVNLTGGKYSIVSWVEKAGVK
                     RYSYGLVLAESLLEHAALRFGDISRVFYEVTTGEKNKDDILTGRGDVVNGQAIVIGGV
                     AVSDSSINLELVFPYGLTARSITPDGSYTYTQTASDSEQYFSTLLADHEDWLASLSPV
                     ATYTAPFNDEITANEDGLIVFKPNDSSGAPYTIYFPDAAGSEHRYLILKYKTESGVFT
                     TKIYAEDFPVHIAIHGNLFIGDSQSDPLKLYYINGQYSITVYGSITVNCQLMYGDFDD
                     EFDEARTQGGKVNNQEINTWSKVKELLNDFSEREAEDHLSLVSIGGDIMNTYVIGNSG
                     KGTHAIRALAGEFSAFKKNGIGGAFTFPDLGTVVKNERTKLGQLFFFGSITGNRFGTI
                     EEMNYLDNLFVSSTRGISGGTFGKRLILAGLRAW"
BASE COUNT         1630 a         1438 c         1671 g         1528 t
ORIGIN      
        1 cttcagatgg aactatcaca cgaaggttac tccgttgaag tgataaccga aggagaaaca
       61 gctttgaaaa ggctggaaaa aaagttaccc gatctcctca tactggacgt catgcttccc
      121 ggagcgaacg gtttcagagt tttgaacaaa gtgagggagt acatatctac agatcttcca
      181 attatcatgt tgacggcccg gggagatgtt gaagaccgtg tacgcggcct taaaggcgga
      241 gccgacgatt atatacccaa acccttcgta atagaagaac tgctggccag gatggaggcg
      301 atattcagac ggaggggaat tctggacagg gtgagttttc acgaaatatc gattgacaat
      361 aatacaaaag aggtaacggt tggcggtgaa ccagttcagt taagtaagac ggaattcgaa
      421 ctccttttcg cgcttctgac gaacgccgga atagtgatgt cgaaggagag actccttgag
      481 aaagtctggg gcagcgaaga atggggaaac ccaaacgtgg tggaggtgta catcaactac
      541 ctgcgcaaaa aactcggaca gtcgggcgag agaatcaaga ctgtgagagg ttctggttac
      601 gttgtcagat aagaggttat tttcaacttc aacacaggtg gccttcaact ccgtggtttt
      661 actggtggtg attctatctg tttcggcgct tttcatatac agggtgtcgg aaagtgtttt
      721 cattcgcaac tactcagaag agatgagaaa gatcttcatg gactctccca tgggtagggg
      781 tatgagaatg gggattggcc gtattatcgg tcgcggtgga acggagttct atgtgaggat
      841 agagggaata gtcgtgcaaa atcctaacgg tctgtcgaac gtacctgatg tggaaggtta
      901 catgaagaca agaatcggcg ggagctctta cctgttctac ggcttcacca acggaaacga
      961 taagattctg cttggtgcgc agatggacga tctggacttc ttcctgcaga gtctttccac
     1021 cacgctggta ctctcgacga tcatcggagt agcgctgtcc atactgagcg gctatctgct
     1081 cggaaggagg gtttcgaaac cgctgagaga ggcaacctcg ctactgaaag aaataacgct
     1141 ggaagacctg tcaagaagag tggagataga tcccaagact tctgagcttg cagagcttaa
     1201 aaaagccctg aacacggcgc tagataggat cgaggacggt tacaaaaggc aggagcagtt
     1261 ctcttcagat atcgcccacg agatacgctc gcctctgacg tcgatactcg gcttttcaag
     1321 aatgatacag aggtggggct ctaaagatcc ggatgctctt aaagaagccg ccgaaagcat
     1381 aacggctact gccggcaaga tgcttaccat aaccgaagga ttgctctttc tggcgcgccc
     1441 ggtaattgaa acgaacagag agagcttcaa cctcggggag ttagtcaacg agctggtgca
     1501 gtccaccccc gtacccggca acataaccgt cgaagtcgat gttgaaaatg tgcaggtcac
     1561 gaccgacagg gctctgctta aaatagcgct aaaggtgttg ctcgagaatg cgatgaaata
     1621 tggaaaagga agacccatcg ttttttactg ggccgatggt tcacttcatg ttagagatca
     1681 cggcgacggt atcaatgagg gggaaataga tagaatattc gatagattct accggagcga
     1741 ttcctcaaga agcggtggag gtcatggtct cggtctttcg atcgtcagaa agatctgtga
     1801 tacccttgac ctgacaatta cggctgaaaa caacgaagac ggaggggccg tcttttcgat
     1861 cgggaggtta ttatgaaaac cctgagctat atcgcagaat caatattact ttggggtgtg
     1921 ctccctctgt ggatactgtc gggaagcgct tccggtatcg gatttttggc cgtggccggc
     1981 ctggtgacgg cggtcgtctc ggcagcattt tcgctgtatt ctacgctggt cgctttctac
     2041 tggacgggaa agttcccggg attgttgacg gttcagaatc aaacggtcac cagtggtccg
     2101 taccgtttta taagacagcc actttatgta ggatactctc tctttctgct tggtgttgtg
     2161 ctgttatcgg ggaagtacct ttttcttgta ctctgggccg gtatctgctg ctgtcttctc
     2221 ctgtacacgc ttttcataga aaaaaaactg gtggatagag acgaacgata cgccaaatac
     2281 agagaaaccg taccgttgct cctgcccgga cgcggcaagt atataccctt cgacttcact
     2341 agatgtgttc cgtggctctt catagcaact tctctcgtgg tgaaattcct tgtactggtg
     2401 ctgctgccct cgaaagtgaa gaacgccagg gtgctgaagg agagaaaacc cttcattatc
     2461 gcacttgcgc accagactca ctacgacggc cccttgctat tttactcgac ctggaggtat
     2521 atcagattcg tcggcacggc gatctacgtg gacaggatga agctactgag aaacttcgga
     2581 acgattcccg tgaaacgcta tacaatagat acctctgcta taagacagat gctgtcaacc
     2641 ataaaggaag gtattcccct cggtatagcg cccgaggcgg cccgttcatg ggacggcaag
     2701 ttcctgagtg taaaaaagga gatctggaaa ctcttcaaga tgctgaaaac tcccatcatc
     2761 ccggtcaaat tctacggaat acagagactg tggccccggt gggccggtat cttctccccc
     2821 ggtttctcca cagtggagtt cggcgatccc gtgcagcctg acgatcccga aatggagcgc
     2881 aagataagcg acttcctctc aaaagaggat cccacctttg ataagcctta cagaagttac
     2941 aggcatatcg agagactcat ctggaggtgc ccctcgtgcg gcaaagtcgg ctctatcgag
     3001 agttttcgta ggggcttttc ctgcaactcc tgcggaaaga gctggaatag accatcggtc
     3061 aatgaggtaa tcgaacttca cgaaaaaatc aagcccggca atatggggct ttcctttccc
     3121 gtgaaagaca gggtcttttt caggggtgaa gaagtagatg ggctgataat ggaagattcg
     3181 gcgatgatag gagatttctc tctcaagtac agcgatataa agaattccag catcgagaaa
     3241 agcattgaac cggtcttcgg aacggccgac gagatggtga tcttcaaatc gaatagtagt
     3301 gcactcatgt ggcaggagat aatcgatttc cagataaagt tccgcctcaa acgcggcgat
     3361 tatcataccg atctctgggg atagttccat caaacagctt cttatggctc atcactcatg
     3421 atcttgagtc tttctccgtt tgccagaatg cgtaagcagg gtgatatgtt aaaatttaat
     3481 aagtaacatt caagcgtgtg gtgttttccg aattttttat tcaatgctga gcgatcacga
     3541 gagcggcatc tcacgacggg tgagtgatta tctttgagat ggtacgggtc ggcaaagggt
     3601 gtttcgcttg tagaaaccgt gatgaatatg ttgttgactg cgatagtcat atcgatagtg
     3661 atggttatgc tgtcaataac tttcaagatc ggttttcaga ttcacagtca attgactatc
     3721 gacgaagaga tcgtgaagtt gagcagcaac ctgaagtaca tcgtctccag gaactggacc
     3781 gggcttgatt tgagtgaagg actcgacggt gatactataa ctctcgaatc gagatttcca
     3841 ctgattcccg aagccagtgt ggcaactatc acatatctcg acggcaggct gatgttcata
     3901 tatagggaaa gtgaaaacac tcttgcccgg acggttctgg ccgagcatct tgatcagttc
     3961 actttttacg acctcgaggg gcagtatctg tattaccggg cggtatttga gtacagagga
     4021 gccaccaaag agatcgacgg ggcggtgagg atctattaga aaggcttttt cgagtgcgga
     4081 aattcttgtc gtgcttgccg tgctggccat gctgatcagt tcggggatcg ccctcattct
     4141 tcagtacaga tccatgtcgg cgactctcga aatctccacc aatctccatt cagccaagct
     4201 gctggtggaa ggtatcgtga ggagtgccaa ccgcgtgagt gaagagaaca taatcgatcg
     4261 aatcgagcag ctatctaatt atccgggctt tcaagacgtt gaagtggctt cggtcgaggc
     4321 gaccaacatc gaggacagcc ctacgagaag gattttcgag gtcgtcctca gggacagaag
     4381 agtcggtgtg gaggaggtgt ttcatgtatt cagattcgat cccttcgcgg aatagaggtt
     4441 tcgttctggc gttgacacta attctgatga ccttcgctac tactatgata atagccgcta
     4501 gtataatcat ccagcgcacc tccaggagac tcaacgagta ttctttgctt tcagacctca
     4561 gggtagcttc gaacaatctg gtggagggag ccgccatggt cctcgccgaa aagtggaacc
     4621 aggacaactt caattccgcc tgggaaggtt tcgaggagtt ccttcagttc gtttcctcaa
     4681 ggggcggcta tgagggagac ctgtggtcgg aagttatcca aactctcgac tctgaaggat
     4741 ggtggttgat caacggagat gaagacttcg cttctctgct agaggacgca tccttttcgg
     4801 agttaacttc cataggggcg gcggtaaacc tcaccggcgg taagtattcg atagtttcgt
     4861 gggtggagaa agccggcgtg aaaagatatt cctacggtct ggtacttgcc gaatcgcttc
     4921 tggagcatgc ggctttgaga tttggggata tatccagggt tttctacgaa gtcaccacgg
     4981 gtgagaagaa caaagacgat atactgacag ggcggggcga cgtggtaaac ggacaggcga
     5041 tcgtgatcgg aggagtagcc gtgagcgact ccagcataaa cctcgagctg gtcttccctt
     5101 acggcctaac cgccaggagc ataacccccg atggatcgta cacgtacaca cagacggcct
     5161 ccgattcgga gcagtatttt tcaactctcc ttgcagacca cgaagactgg cttgcgtccc
     5221 tctctcctgt ggcaacgtac acggcgcctt tcaacgacga aatcacagcc aatgaagacg
     5281 gcttgatcgt tttcaaaccc aacgacagca gcggcgcacc ctacaccata tattttcccg
     5341 acgccgcggg gagtgaacac aggtatctca tactaaaata caaaactgag agcggagtat
     5401 tcaccacaaa aatttatgcc gaggacttcc cggtgcacat cgccattcac ggaaacctgt
     5461 ttataggaga ttcacaaagc gatcccctca agttgtacta tataaacgga cagtacagca
     5521 ttaccgtcta tggttcgatt accgtgaatt gccagctgat gtacggcgat ttcgacgacg
     5581 agttcgacga ggccagaacg cagggaggca aggtcaacaa ccaggagatc aacacctgga
     5641 gcaaggtcaa ggaactgctg aatgacttct cagagcgcga ggcagaggat catctctcgc
     5701 tggtcagtat aggtggagac ataatgaaca cttatgtgat cggtaactct ggcaagggca
     5761 cccacgctat cagggctcta gcgggggagt tttcagcctt caagaagaat ggaatcggcg
     5821 gtgccttcac cttccccgat ctgggcaccg ttgtcaaaaa cgaaaggacc aagctgggcc
     5881 agcttttctt ttttggatct atcacgggta accggttcgg caccatagaa gagatgaact
     5941 atctggacaa cctctttgta agctccacca ggggcatatc tgggggaaca ttcggtaaac
     6001 gtttgattct agccggtttg agagcatggt aggaaatggg attggtctat atgccaatga
     6061 acgccttcgc cgccgatagg aaactataca gaccttcgtt atcgccttcc tctctgacaa
     6121 ccacaatgaa aaggtggggt ctcccccacc cgtatcagat ttgccacatc aagtctttcg
     6181 ttctctcgta gagctggcgg aatagcgcgt aagatttttc gtatatctcg cgattattcg
     6241 ggtcgggatt gatcggttct ctgaact
//
LOCUS       DAOOLY010000141         6257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_14872_length_6257_cov_4.544663, whole genome shotgun sequence.
ACCESSION   DAOOLY010000141 DAOOLY010000000
VERSION     DAOOLY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14872_length_6257_cov_4.544663"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..881)
                     /locus_tag="PLD15_07765"
     CDS             complement(135..881)
                     /locus_tag="PLD15_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB63841.1"
                     /translation="MTRGEVMGETPVPLYYKLYVELKEGLQSGKYKKGDKLPTEKELC
                     QKHGLSRLTVRRAMDELRREGFIERLKGKGTFVTGSKREEQLATLTGFTDEAKKRGCE
                     TRSVVLENRLVKVPVDAVEFFKIPADALVVYLKRVRLLEEEPYAIEEAYLNVGADIRL
                     LNIVQRDMEKESLYDILRNEFEINISYAEEEMELARLKKDEARFLKQEMNDCAILRKR
                     FTYTKSDVCVEFVVSLYRADKSKFKIVRRI"
     gene            complement(878..2005)
                     /locus_tag="PLD15_07770"
     CDS             complement(878..2005)
                     /locus_tag="PLD15_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPB63842.1"
                     /translation="MSFLVLQGIDILSRRDFADLKDLSIGLITNYSFIDNSLRCGIDM
                     MFERGLKIRKIFTPEHGLGGIADGAVYDDSIHPRYNVPVVSLYGSKRKPRTEDLDGLD
                     ILVYDIQDVGLRYYTFIYTLAFTMEAAAENGKRYMVLDRINPLGRGVFGSRIENDLQT
                     FVGGYRLPLQYGLTSGELARYFKKLGGLDLDLRVVPAEGWKGGSFDETSLVWNVPSPN
                     IPTFESLLGYAGTCFFEGTNVSEGRGTFKPFLVIGAPWIDGFGMTEKIKGDFPSVKVR
                     RREFMPFYRKYANASCDGVEFFPSKSDNYFLLALSVMEYLLKYEKFEISDRLDDLMGV
                     KESSRKIRSGSLNPDQWESSGEEFIEFVQDCLIHPGRLFYR"
     gene            complement(2155..3393)
                     /gene="gntH"
                     /locus_tag="PLD15_07775"
     CDS             complement(2155..3393)
                     /gene="gntH"
                     /locus_tag="PLD15_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis MBL fold
                     metallo-hydrolase GntH"
                     /protein_id="HPB63843.1"
                     /translation="MVGLIRRKVLRSLVLCVIMAIASCAFGYATYSGFPGQIISAFEP
                     FIPGEQLADDQMRITFMGTSVVPRIAQQCNSVFVELGNGDSFVFDFGSGVSSNYVAAG
                     IPPSRMDKVFLTHLHGDHVGDLITLYCFGPSQDRKTPLYLYGPSGDDPEEGTQAFAEN
                     LKKLMKWHVEAFSFLSTGLKDGRDGYEIIAKELPYMTVGGVAYEENGVKITHFPAVHD
                     RNGSISYKLEWNGLSMIFSGDTIPNYYMIEQAKGIDVLIHEMVVPPEVWASKNSGLNP
                     GDPGWERAVAFALAVQRNSHTSQKAFGYILSQTNPRLGVATHFQANEDTVGPAIEDIR
                     LSYEGPVAIATDLLVINVSKNDILLRRAVVSDYAWYPQPYMYPQDQIAPPKYDGPFAQ
                     LNDTLLGSIVPEEIYEVGDK"
     gene            3655..4068
                     /locus_tag="PLD15_07780"
     CDS             3655..4068
                     /locus_tag="PLD15_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63844.1"
                     /translation="MTVNLINIFTFLMFLSRVKWPKVEILFGTATIILALPAVTIGAL
                     NLLRSANFLLWFPAILYGFWALLALVVDYLLKIEFRNPAKPSILVPFLILFYFSVGGM
                     AAAFYRIDLYLWLISGVTSILNLYGAYYAGKQGKG"
     gene            4380..5702
                     /locus_tag="PLD15_07785"
     CDS             4380..5702
                     /locus_tag="PLD15_07785"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HPB63845.1"
                     /translation="MKLRSHLIFWGSLALISWGLSRFNYLFYHSTVEIFTISVAMMLV
                     AVALIARSKNNIVFRLGSLYAVVMVFDILHTLTFKGMSVFQNWSANQSTQFWILGRSL
                     EALGLALAIVLPNCKRCNIFYWIFVAASGITGILLVGSGVFPDCYLAETGLASFKIIM
                     ELVILGVIAFTIFLLMKTDNKEIRPYKNYYLTALFFTALAELFFTIYTDPYGFTNTLG
                     HIFRLISYFVILEGIVVRSFTEPLDTLYNDMNTMVEELTSIISETTEIKDPYTAGRQK
                     RVAALSEEIAKKMHLSGERRKELVLASRLHDVGTLFVPTDIISKVTPVSQIEYNLIKQ
                     HVIKSYELLKGLPLSWRIFEIIRQHHERIDGSGYPRALKGDEILLEAKILAVADVLEA
                     MNYDRPHRKAPGMEKAVEELKRNSGILYDARVVEVCIEVIDGGFKFDG"
     gene            6057..>6257
                     /locus_tag="PLD15_07790"
     CDS             6057..>6257
                     /locus_tag="PLD15_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63846.1"
                     /translation="MKKVYVYFAVALLLILITGCPSFWKAADATLKQLMYNGISVPGF
                     DAKKTGYSVELPAETTEPPTVTA"
BASE COUNT         1534 a         1582 c         1422 g         1719 t
ORIGIN      
        1 tgaaacgaag atcgcttatt ttcatttgct cctcccgatt aaagaaagac tttgacgaaa
       61 aaactataac tatgatactc catagatggc acagccaaaa cagagggatt taatcagata
      121 ataccatcgc tttcttatat tcgccttacg atcttgaact tactcttatc ggcccgataa
      181 agagaaacga caaattccac gcagacatcg cttttggtgt aggtgaagcg ctttctcaag
      241 atcgcgcagt cgttcatttc ctgcttcagg aagcgcgcct cgtctttctt cagcctcgca
      301 agttccatct cttcttcagc gtaggagata tttatttcga attcatttct tagtatatcg
      361 tagagcgatt ccttctccat atcgcgctgg acgatgttca gcagtcttat atcggcaccc
      421 acattcagat aggcttcctc gatcgcgtac ggctcctctt cgagcaacct gaccctcttc
      481 agataaacca cgagagcatc ggccgggatt ttgaaaaact ccacggcatc gaccggcact
      541 tttaccagac ggttttcgag caccaccgac ctggtctcgc agcccctttt cttggcctca
      601 tcggtgaaac cggtcagcgt cgccagttgc tcctctctct tggagcctgt gacaaaggtc
      661 cctttgcctt tgagcctttc gatgaacccc tctcttctca gctcgtccat ggctctcctg
      721 accgtgagcc ggctgagccc gtgtttctgg cagagctcct tttcggtggg aagtttgtca
      781 cccttcttgt acttccccga ctggagccct tccttcaatt cgacgtacag tttgtaataa
      841 agcggaacgg gcgtttcccc catgacttcc cctctcgtca tctgtagaaa agtctccccg
      901 gatgtatgag acagtcctgc acaaattcga tgaattcctc tcccgaactt tcccattgat
      961 cggggttgag tgatcccgac cttattttcc tcgaactttc tttcactccc ataagatcgt
     1021 cgaggcgatc ggaaatctcg aatttctcat attttagcaa atactccata acacttagcg
     1081 ccagcaggaa ataattgtcg cttttcgacg ggaagaattc gacaccgtcg cagctcgcgt
     1141 tggcgtattt gcggtaaaag ggcatgaact ccctccttct gaccttcacg gacgggaagt
     1201 ccccttttat tttctcagtc atgccgaagc catctatcca gggcgcccct ataacgagga
     1261 agggtttgaa ggtcccccgg ccttccgaga cgttggtccc ttcgaaaaag caggtgccgg
     1321 cgtacccgag aagagattcg aaggtcggta tgttcggcga gggaacgttc cacacgagcg
     1381 aggtctcatc gaaagacccg cccttccagc cttcggccgg taccacacgc agatccaggt
     1441 cgagcccacc cagctttttg aagtacctgg ccagctctcc cgaagttagc ccgtactgga
     1501 gtggcagccg atagccaccc acgaaagttt gaagatcgtt ttctattctg gagccgaaaa
     1561 cgcccctccc cagaggattg atccggtcca gcaccatgta tcgcttcccg ttttctgcag
     1621 ccgcttccat cgtgaaggca agagtgtaga tgaaagtgta gtagcggaga ccgacgtcct
     1681 ggatgtcgta aacgagtata tccagaccgt cgagatcctc ggttcgcggt ttacgcttgc
     1741 tgccgtagag actcaccacc ggcacgttat acctgggatg gatactatca tcgtacacgg
     1801 ccccgtcggc tattccgccc agcccgtgct cgggagtgaa gatcttgcgt atcttcaaac
     1861 ccctttcgaa catcatatct atcccgcagc gaagagagtt gtcgatgaac gagtagttag
     1921 ttatcagacc aatcgataga tctttcaggt cggcgaaatc ccgtctcgac agtatatcta
     1981 tcccctgtaa taccaagaag ctcacctcca aacgaaatct ctcaagtaca ataatgccat
     2041 attgtcatgc catttagtat ctttctatcg tgtcctcatt aatactccag ctaaaaaaag
     2101 gccgatctct tcgtcggcct ttttcctgtc ttactggcgg tagtatctgg ctttttattt
     2161 atccccaact tcgtagattt cctcgggaac aatgcttccc aggagcgtat cgttaagctg
     2221 ggcgaacggg ccgtcatatt tgggaggagc gatttgatcc tggggataca tgtacggttg
     2281 cggataccag gcgtaatccg aaaccactgc tctgcgaaga aggatatcgt tctttgagac
     2341 atttatcacg agcaggtcag tggcgattgc gacgggccct tcataagaca ggcgtatgtc
     2401 ttcaatcgca ggcccgacgg tatcttcgtt ggcctgaaag tgcgtggcga ccccgagcct
     2461 cgggtttgtt tgactgagta tatagccgaa ggctttctgt gaggtgtgag aattcctttg
     2521 cacggccagc gcaaatgcca cagccctttc ccagccgggg tctccaggat tcaatcccga
     2581 gtttttactg gcccacactt cgggcggcac aaccatctca tggatcagaa catcgatacc
     2641 cttagcctgc tcgatcatat agtaattggg tattgtatcg cccgagaaga tcatcgaaag
     2701 accgttccat tcgagtttat agcttatgct tccatttctg tcgtgaacgg cgggaaaatg
     2761 tgtaattttt acaccgttct cctcgtatgc gactccgccg actgtcatat acggcagctc
     2821 cttcgctatt atctcgtaac cgtcgcgacc gtctttcagc cctgtgctca aaaagctgaa
     2881 agcctccacg tgccatttca ttagtttctt tagattctcg gcgaacgcct gagttccttc
     2941 ttcgggatca tctccggaag gtccatacag atagagagga gtcttgcgat cttgagaggg
     3001 accgaaacag taaagcgtta ttaaatctcc aacatggtct ccgtgcagat gagtgaggaa
     3061 aaccttgtcc atgcgtgatg gtggaatgcc ggccgcaacg taattggatg aaacgcctga
     3121 tccaaaatcg aatacgaagc tgtctccatt acccagttcg acaaacacgc tgttgcactg
     3181 ctgggcgatt ctcggcacaa ccgatgtccc catgaatgtt atcctcatct gatcatctgc
     3241 gagctgctca ccgggaataa aaggttcaaa agcgctgatt atctgtcctg gaaaaccact
     3301 ataggtggcg tatccgaaag cgcaggaagc tatcgccata ataacgcata aaaccagaga
     3361 tctcaaaact tttctccgaa tcaatcctac catcgttctc cctcctactt ttccttgagt
     3421 ctttcacttt tgaattgctg taactccatg gaattttccg taaaactata gatacacaca
     3481 cttcgctgtg aatgaattga accttataga atatataaca gccgtatgcc cgatatctcc
     3541 gaagacctac tgtgaaattt cgatcactgt tttgcgctgt cgatagtata atactgctcc
     3601 gcgcgcgtga taagatacta accggaggcg atcgggatgc aattaccgct cttcatgacg
     3661 gtgaatctga tcaacatatt cacttttctg atgtttctat ctagggtaaa gtggccgaaa
     3721 gtcgagatct tgttcggaac ggcgacgatc atcctggctc ttcccgcggt gactatcggc
     3781 gctctcaacc ttttgaggtc ggcaaatttc cttctctggt tccctgcgat tctttacggt
     3841 ttctgggctc tgctcgcact ggttgtggat tatcttttga agatagagtt caggaatccc
     3901 gctaaaccat ccatactggt acccttcctg attctttttt acttctccgt cggtgggatg
     3961 gctgcggcat tttacaggat tgatctgtac ctgtggctca tctcgggagt aacctctatc
     4021 ctcaatctgt atggagctta ctacgccggt aaacaaggca aaggttgatc tctatattga
     4081 atggaacaat acatgatttt gaaataatga ctattttaca gaattagtga tcttgattct
     4141 cctggatgca taagtctgtt cgtcaactgt ggtgaaaatc atcacattga aattgatttt
     4201 ggcaacggaa atcagtgaat catggaagta ctatcttcaa tgcttaataa caccttttca
     4261 ttctctcgat tgacttgtgt ggataacata caagattgaa ccgtgatttt gatagaatta
     4321 cggcatatcg acagtgattt ttcaaaggct ccggtctctt tttaatcggg aggtaagcga
     4381 tgaagctgag gagtcatctt atcttttggg gttctctagc tttgatttcg tggggattgt
     4441 cgagattcaa ctatcttttt taccactcta cagtcgagat attcaccata tctgtcgcaa
     4501 tgatgctggt ggccgtcgcc ttaattgcca gaagcaagaa caacatcgtg ttcaggctgg
     4561 gcagtcttta cgctgtggtg atggttttcg atatacttca tacgctaacc ttcaaaggca
     4621 tgagtgtctt tcagaactgg tccgccaatc aatcgacgca gttctggata ctcggaaggt
     4681 ctctggaagc cttgggcctg gcgctggcaa tagtgttgcc aaattgcaaa aggtgcaaca
     4741 tcttctactg gatatttgtc gcggcttcgg gcatcacggg tatcctgctg gtgggaagtg
     4801 gcgtttttcc cgactgctac ctcgccgaaa ctggtctggc ttctttcaaa ataatcatgg
     4861 agctggtgat ccttggagtc atagcattca cgatcttcct gctcatgaaa acggacaaca
     4921 aagagatcag accgtataaa aactactatc tgacggcact tttcttcacc gccctggccg
     4981 aactgttttt cacaatttac acagaccctt acggtttcac taatacactg ggacatattt
     5041 ttaggctaat ctcctatttc gtgatactgg aagggatagt cgtgcgatcc ttcaccgagc
     5101 ctctggatac gctctataat gacatgaata ctatggtgga ggaactcacc agtatcatta
     5161 gtgaaaccac cgagatcaag gatccgtaca ccgccggtcg ccagaaaaga gttgcggcgc
     5221 tttcggaaga gatagccaaa aagatgcacc tctccggcga gagaagaaag gagctggttc
     5281 ttgcgagcag attgcacgac gtgggcactc tcttcgtccc cacggatatc atctcgaaag
     5341 tcacacctgt atcgcagatc gaatacaacc tgataaagca gcacgtgatc aaatcctacg
     5401 aacttctcaa gggcctgcct ttgagctgga gaatcttcga aataatacga cagcaccacg
     5461 aaaggatcga cggttcggga tatcccaggg cgctcaaagg agatgagata cttctggagg
     5521 ccaaaatcct ggcagtcgcg gatgtactgg aggctatgaa ctacgacaga ccacaccgta
     5581 aggcaccggg aatggagaaa gccgtcgaag agctgaaaag aaactcgggc atattgtacg
     5641 atgccagggt tgttgaagtc tgcatagagg tgatcgacgg cggcttcaaa ttcgacggat
     5701 agtcgttcaa agtcaatcca gtgctttcaa cagatgaccc ctgctgaacc gcccccttct
     5761 tttaaggctg acttccaaca tctgaaaggc gtgaacccat gtttgatcgt tagttggctc
     5821 tttgggtagt cttagatctt ttctgaattg ggtttattta tttaaaactt cttcgagatt
     5881 atatacctga gatgatagac ttaaaacaaa gaatgtacta acgccgccct cttttcactc
     5941 tacacagaac ttttctatca tttttttgct cacggtacgg tgatgataag gctattagag
     6001 atctacaatg gctttttcga ttcattaata tcactcttca cgaaggaggc atgaaaatga
     6061 aaaaggtata tgtgtatttc gcagttgcat tgcttcttat attgattacc ggctgcccgt
     6121 ctttctggaa agcggccgat gccactctga agcagttgat gtacaacggg ataagtgttc
     6181 ctggctttga tgccaaaaaa accggttatt cggtcgaact tcccgcggag acgacagagc
     6241 caccaacggt gacggcc
//
LOCUS       DAOOLY010000142         6220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15000_length_6220_cov_4.162855, whole genome shotgun sequence.
ACCESSION   DAOOLY010000142 DAOOLY010000000
VERSION     DAOOLY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15000_length_6220_cov_4.162855"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLD15_07795"
     CDS             complement(<1..302)
                     /locus_tag="PLD15_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HPB63847.1"
                     /translation="MDIDEKVGQKIEKWKRDLLDFSMRNRLINFRYGKTGTLKLLSPS
                     VNVIFETLAVRERVISFVPTPPRESGNFTDDFSGHLKSGQVLTEREEKEMNLVLNRL"
     gene            866..2029
                     /locus_tag="PLD15_07800"
     CDS             866..2029
                     /locus_tag="PLD15_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPB63848.1"
                     /translation="MSKLMELRTEEILRITREKKRTSRVQLAKVTGLSKPTVSSIVND
                     LVRKGILTETGLGKSKATGGRKPINLSFSPDFKYVLSVDIGGTKAIFALLDLDGNILV
                     RETVPSELFRNGKGILKELEERLERYIKKAGREKILGITLGIPGTVDRSSQVIKYMPS
                     FDQGDLDLKSPLEDKYKISVLVENDVTLAAFGESWIGAAREYGDVVLVSIGTGIGAGI
                     VIDNTVYRGYAGSAGEIGEFVTDWTLESGESSGFGRLENWFSGHSLEAFCKENDLPIT
                     VEGLFERMESDEKTRERIVNGCEHLALAFANSLMLLDPAKLLIGGGIGFNQYDRIFPI
                     IDDTLKRVLPAELYRPDLLSRAVLEPYSVVIGGAYFAQKELLLREVLGGASRE"
     gene            2062..2985
                     /locus_tag="PLD15_07805"
     CDS             2062..2985
                     /locus_tag="PLD15_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB63849.1"
                     /translation="MKKAVFVILLLVMAVAVVLATDVAVLLPGTVEFFSVQRRGLDAA
                     AQEFGLNLIYADAEWDAGKQLSQVENFIARKVDLVLLCAADNMALRTAVTRCNEANIP
                     IITFTNSIGTDPEGKYPGVISHIGRSEIGSGVVQAEFVEKLFGNASIDIILIEGNPGT
                     TAQRMREEGFISIANKYPNWKIIEKRPIDGWTKEGTLAFMEAFLQSGRNVDVIACQWW
                     SGAIAASMALKERGISDVYVLGLEYARELVPYIESGDVYATTYFSVVEEGYKAVEAAH
                     KYLTGQEVPKFVEIKQIIVTKDNVKEFEPEM"
     gene            3024..4241
                     /locus_tag="PLD15_07810"
     CDS             3024..4241
                     /locus_tag="PLD15_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63850.1"
                     /translation="MSLAERVKILSQLFLNEELTPDLADRLKRKLEKMKRLGLEALMI
                     WSKDTDNLGRLASICAREGVSPYLWYPMLADTPARFEAKDFQVVAAVESGRSQILEAQ
                     KAGGEEFAFLCPNRVGGEERFLELFEETLDLADFAGVFFDRIRFPSPANGLKEILTCV
                     CDDCLERSGGVIESLRGDLQRLLKEPGKAVGVEAMPGILRELFASIEEAIVFRTTSVS
                     ALLADYARAARKRNLDIGIDLFPPSLARLVGQDYTELSRHVDWIKPMIYCKTMGPAGL
                     PMELLSLAKILRELNGNISERDSLWVLEQLTGLDLPESFGELASKGLTLDNYDRELEK
                     LESLDRGGAVRIYPGFEAVAFPPVCSVDPAIVGEYVRRTLNRGYRGFTLSWDIRQIPP
                     ANLEAVGDFLAGW"
     gene            4228..5769
                     /locus_tag="PLD15_07815"
     CDS             4228..5769
                     /locus_tag="PLD15_07815"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB63851.1"
                     /translation="MPGGKSGTRNIIEIRNFSKSFPGVQALKDVSMDIRVGEVHALLG
                     ENGSGKSTLTKCIVGAYHRDSGTMIYDGVESSFLSPAESFKNGISVIYQERSLIPKLT
                     VEQNVFLGDELLSAGLLSGKQMRKRYTALCEKFGFDLPPDEKIYGLGVAQQKLVEIMK
                     ALSRNSKVVIMDEPTASLSKQEADHLFKIIRDLREKEISILYITHILEEVFRITDRIT
                     ILRDGRKVSTLDTAQTGKEEIVNLMVGEVFHDAEYISSYASYDRQPVLSAKNVVKLPR
                     VRDISLDIYPGEVLGITGLTGSGKTELARVLFGAEKIESGEIFVEGRRVDLNSPVDAI
                     RAGIALIPEDRKSDGLVLLLEVFKNITLPAIGEFTVGPGLLSVRREYVKSQEYHKRLN
                     IRSASMNQQTKYLSGGNQQKVVIAKWLLTNPKLLIMDEATQGIDVKAKSEIYGIVREL
                     AESGFSVLFISSEVQEVLRVSDRILVTRDGEIAGEFGRGTRQDEILRTTLGGKIESSN
                     RQVVR"
     gene            5770..>6220
                     /locus_tag="PLD15_07820"
     CDS             5770..>6220
                     /locus_tag="PLD15_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HPB63852.1"
                     /translation="MSNPGASSKITGFLGKYKILIAFLVLIIVISFMTPNFLSWRNII
                     NVFRQSSIIGIMAIGATFVIIGGGFDISVGSLLALTAAMVVGLQSYMHWFPAMLVVLL
                     VGAAFGVANGFLTSKIHIPPIIATLGTMTIIRGIVYMYTGGYPLYVDS"
BASE COUNT         1592 a         1507 c         1742 g         1379 t
ORIGIN      
        1 agtctgttca gaaccaggtt catctccttc tcttcccttt cggtaagtac ctggccggac
       61 tttagatgtc cggaaaagtc atccgtgaag ttgccgcttt cacgtggggg agttggaacg
      121 aagctaatca ctctctccct aacggcaagg gtttcgaaga taacgttaac agagggagag
      181 agaagtttga gcgtgccggt ctttccatac ctgaaattta tcaatctgtt tctcatagag
      241 aaatcaagta gatccctctt ccacttctcg atcttctgcc ctaccttttc gtcaatatcc
      301 atgactcacc ccgcttgcat cgaacggtct tgcagaataa aaaaaccgtg cgacgaatct
      361 tttaatgccc ttgattcaag gcgtttcaag gtttgataat aaacgacaac tcccgttccg
      421 gacagacccg tatgctccac gaagctaaaa gccgattgca aaaagaagag attgccgtat
      481 aatactctag cacttataac cggaaaatag aaaacacgaa aaagagcgcg tattttccac
      541 aaacagtccg cgatcgagtt gctggttttc caacaatcct gacacgtctt agcgttcgcc
      601 tacatacaac taacaatgtt tacttacatc cggtggctag tagatcgacc gccgttctct
      661 cggcctttcc tttgtcgttt gtgaagaatg agctataatt tcaccgaaat tggttagtat
      721 tttttattta ccaatgttgt gtataaattc ccgcagtaat cgaagaaacg ttcttaacag
      781 gatagatcaa gcaaaattcc cgccgaatct cactttgact gtggaagctg aacgtttcgg
      841 ccggaggttc tggaggaagc gagtggtgtc gaagctcatg gaacttagaa cagaagaaat
      901 tttgagaata acgagggaaa agaagcgaac atcgcgcgtg cagctcgcga aagtcacggg
      961 cctcagtaag ccgacggtct cttccatcgt caacgatctg gtgagaaaag gcattttaac
     1021 ggagaccgga ctagggaaga gcaaggccac cggcggaagg aagccgatca atctctcctt
     1081 ttccccggat ttcaaatatg tattgagcgt tgatataggc ggcacaaagg cgatattcgc
     1141 cctgctggat ctcgacggta acatactcgt tcgcgagacg gtcccgtcag agctgttccg
     1201 caacggaaag gggatattga aggagctcga agagaggctg gagagataca taaagaaggc
     1261 cggcagagaa aaaatactcg gtattacgct gggaataccg ggaacggttg acagatcttc
     1321 tcaggtcatc aaatatatgc cttcgttcga tcagggggat ctcgatctca aatcgcccct
     1381 ggaggataag tacaaaatct ccgttctcgt cgaaaacgac gtgacactgg ccgccttcgg
     1441 cgaatcctgg atcggggcgg cgagggagta cggcgatgtg gttctcgttt ccatcggtac
     1501 gggaattggc gcaggtatag tcatagataa cactgtttac aggggttacg ccggaagtgc
     1561 cggcgagatc ggggaattcg tgacagactg gactctggaa tcgggtgaaa gttcgggctt
     1621 cggccgactg gaaaactggt tctccggcca ctcactggaa gcgttctgta aggagaacga
     1681 tctgccgata acggtcgaag ggcttttcga gagaatggaa agcgacgaaa agacaagaga
     1741 aagaatcgtg aacggctgtg agcatctggc gctggctttc gccaactcat tgatgcttct
     1801 ggatcctgcg aagttgttga ttggaggtgg tattgggttc aaccagtatg accggatctt
     1861 tccgatcatc gatgacaccc tgaaaagagt cctgcccgcg gagctctaca gaccggatct
     1921 tttgagcagg gccgttctcg agccttacag cgtggtcatc ggtggcgcgt acttcgctca
     1981 gaaagagcta cttctcagag aggttcttgg gggtgcttca agagaatgag aaaaggcaat
     2041 ccaaagacag gaggagtgaa tatgaagaag gcagtttttg taattctttt acttgttatg
     2101 gccgtagcag tggttttggc gactgatgta gccgttcttc taccgggaac cgtcgagttt
     2161 ttcagcgtgc agcgcagagg tctcgacgcg gcggcccagg agttcggact caatctgatc
     2221 tacgccgatg ccgaatggga cgccggcaaa cagctttcac aggtcgagaa cttcatcgcc
     2281 agaaaggtcg atctcgttct gctctgtgcg gccgacaaca tggcgctcag aacggccgtc
     2341 acccgctgca acgaggcaaa tatcccgata atcactttca ccaacagcat cggaaccgat
     2401 cccgaaggaa agtatcccgg tgtcatctct cacatcggac ggtccgagat cggttctggg
     2461 gtcgtgcagg ccgagttcgt cgagaagctc ttcggaaatg cttcgataga cattatactc
     2521 atcgagggca accccggcac aacggctcag agaatgcgtg aagaggggtt catttcaatt
     2581 gcgaacaagt acccgaactg gaaaataatc gagaaacgac cgatagacgg ctggacaaaa
     2641 gaaggaactc tggctttcat ggaagccttc ctccagagcg gtagaaatgt cgatgtgatc
     2701 gcctgccagt ggtggtccgg cgccatagcc gcttcgatgg ctctcaaaga gagaggcata
     2761 agcgatgtct acgtcctcgg actggagtac gcgagggaac tggttcccta catcgaatcg
     2821 ggcgatgtct acgcgaccac atatttctcg gttgtagaag agggttacaa agcggtagag
     2881 gccgcacaca agtatctcac cggtcaggaa gttccgaagt tcgtcgagat taagcagatt
     2941 atagtgacca aagacaacgt gaaggaattc gaacctgaga tgtaattgga tgtaattgag
     3001 cgaacggaaa cggaggtcga ttcatgtcgc tagcggaaag agtgaagatc ctttcgcagc
     3061 tttttctgaa cgaagagctt actccggatc ttgccgacag gctgaagaga aaactggaaa
     3121 aaatgaaacg tctgggactg gaagcactga tgatatggtc caaagatacg gacaatctcg
     3181 gccggctggc ttcgatttgc gcccgcgaag gtgtctctcc gtatctgtgg tatcccatgc
     3241 tggcggacac accggcgcgt ttcgaggcaa aagatttcca ggtggtggcg gcggtcgaaa
     3301 gcggccgttc ccagatactt gaggctcaga aggccggcgg agaggagttc gcctttttgt
     3361 gtccgaacag ggtaggggga gaggagagat ttctcgagct cttcgaggag actctcgatc
     3421 tggcagattt cgcgggggta tttttcgaca gaataaggtt tccctctccg gccaacggat
     3481 tgaaggagat cctcacctgc gtgtgtgacg attgcctcga gcgatcgggg ggcgttattg
     3541 aaagtctgcg aggggacctg caaagacttc tcaaagagcc gggaaaggct gtcggcgtag
     3601 aggccatgcc ggggattctc agagaacttt tcgcttcgat cgaagaggcg atcgtatttc
     3661 gaacgacctc cgtttccgcg cttcttgccg attacgcaag agcggcgcgc aagaggaacc
     3721 tcgatatcgg tatagatctc tttccgccat cgctggcgag gctggtcggt caggattaca
     3781 cggaactgtc caggcacgtt gactggatca agcccatgat ctactgtaaa accatgggac
     3841 cggccggcct tccgatggaa ctcctgtcgc tcgcgaagat acttcgagag ctcaacggca
     3901 acatctcgga gagagactcc ttatgggtcc tcgaacagct gacggggctc gaccttccgg
     3961 aaagcttcgg agagctggcc tcaaagggac tgacactgga taactacgac agggaactcg
     4021 aaaagctgga gagtctggac cggggcggcg ccgtcaggat ctatcccggc tttgaagcgg
     4081 tggcttttcc gcccgtctgc tccgtcgatc cggccatagt tggagaatac gttcgtagaa
     4141 cactgaacag agggtataga ggattcacgc tatcgtggga tataagacag ataccgcccg
     4201 cgaacctcga agcggtgggt gattttcttg ccgggtggta agagtggaac cagaaatatt
     4261 atcgagatca ggaacttctc gaagagcttt cctggagttc aggccctgaa agacgtgagc
     4321 atggatataa gggtcggaga agtccacgcc ctgctggggg aaaacggttc gggcaaatcg
     4381 actctcacca agtgcatagt aggagcctac cacagagatt cgggaacgat gatctacgac
     4441 ggcgtcgaga gcagctttct ctccccggcc gagtctttca agaacgggat cagcgtcata
     4501 taccaggagc gcagcctgat tccaaagctc actgtcgagc agaacgtctt cctcggggat
     4561 gagctactga gcgccggtct tttgagcggc aaacagatga ggaagagata cacggcactc
     4621 tgtgaaaagt tcggtttcga tctcccgccc gacgaaaaga tatacggact cggtgtcgcc
     4681 cagcagaagc tggtcgagat catgaaggct ctttccagaa attcgaaggt cgtcatcatg
     4741 gacgaaccga ccgcatcgct ctcgaaacag gaggccgatc atctattcaa gatcatacgt
     4801 gacttgaggg aaaaggagat atcgatcctc tacataactc acattctcga ggaagtgttc
     4861 aggataaccg atagaataac catcctgaga gacggcagaa aggtatccac actcgacacc
     4921 gcccaaaccg ggaaagaaga gatcgtgaac ctcatggtag gggaggtctt ccacgatgcc
     4981 gaatacatat ccagctacgc cagctacgac cgccagccgg tgttgtcggc caaaaacgtc
     5041 gtgaaacttc caagggtgag ggatatctcc ctggatattt atcccggcga agtcctcgga
     5101 ataaccgggc tcaccggttc gggaaagacc gagctggcca gagtgttgtt cggcgcggag
     5161 aagatcgaga gcggggagat attcgtcgag ggaagaaggg tcgatcttaa ttcgccggtc
     5221 gacgccattc gcgcggggat agccctgata cccgaggaca gaaaatccga cggtctcgtg
     5281 ctgctcctgg aggtcttcaa gaacataacc ctgcctgcga taggagagtt cacggtcggt
     5341 cccggactgt tgagcgtccg cagggagtac gtaaagtcac aggaatacca caagagactc
     5401 aacatacgtt ccgcgtcgat gaaccagcag accaaatacc tgagcggcgg gaaccagcag
     5461 aaagtggtaa tcgctaaatg gcttctcacc aacccaaagt tgctcataat ggacgaggcg
     5521 actcagggta tagacgtgaa ggccaaaagc gagatctacg gcatagtgag ggaactggcc
     5581 gagagcggct tctccgtgct tttcatttct tcggaagtac aggaagtgct gcgcgtgtcc
     5641 gacaggatcc tcgtgaccag agacggagag atcgccggag agttcggacg cggcacccgt
     5701 caggacgaga tcctcagaac gactctgggc ggcaagatag aatcatcgaa tcgacaggtg
     5761 gtgagataaa tgtcaaatcc cggcgccagt tctaaaataa ccggctttct gggtaaatac
     5821 aaaatcctga tagcctttct cgtgttgata atagttatat ccttcatgac ccccaacttc
     5881 ctgtcgtggc gaaatataat caacgtgttc agacagagtt ctataatcgg aataatggct
     5941 atcggagcca cttttgtcat cattggcggc ggcttcgaca tttccgtcgg atcgcttctc
     6001 gcgcttacgg ccgcgatggt tgtgggcctc cagtcgtaca tgcactggtt tcccgcgatg
     6061 ctggtggtcc tgctcgtggg ggcggccttt ggggtcgcga acggttttct gacctcgaag
     6121 atccatatcc ccccgataat agccaccctt ggaacgatga cgatcatcag gggcatagtg
     6181 tatatgtaca ccggaggtta cccgctgtac gtggattccg
//
LOCUS       DAOOLY010000143         6212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15032_length_6212_cov_4.551892, whole genome shotgun sequence.
ACCESSION   DAOOLY010000143 DAOOLY010000000
VERSION     DAOOLY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15032_length_6212_cov_4.551892"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1184
                     /locus_tag="PLD15_07825"
     CDS             195..1184
                     /locus_tag="PLD15_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB63853.1"
                     /translation="MKYRKVKKIEEKLSTLGYGCWGISGPSFWDGTTDDDSIKTIRKA
                     VGEGINFFDVAPVYGFGHAEEVLGKAIKGMRNDIFIATKCGLVWDQQNRISNCLKPES
                     IRREIEASLRRLQTDRVDLYQLHWPDPRVDIEETMDAVNELKKAGMIRYIGLSNFSLS
                     LAERAMKVSDIASMQGLYNMLERNPKSYHNIPLEYAVEKEVLPFVKENGMAFLPYSPL
                     FQGLLTGTFDLERENFSSSDVRSANPKLSGPDFEKYYSVVKELNAISGEIGKPLSQIA
                     INWLLYHKEVTSIIAGAQNIAQLEQNLGAVEWEIDNGLYGRIETLVKNSGIDL"
     gene            1193..1678
                     /locus_tag="PLD15_07830"
     CDS             1193..1678
                     /locus_tag="PLD15_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB63854.1"
                     /translation="MKAAYVKAPFQFQIREVELPEPGPNDVLLSIKASGICGTDLHIA
                     RTEARDWQPFGHEIVGLVEKIGSNVGNVSLGDKVVVESGSFCGKCADCRNGRVDLCHD
                     RTPNVFSRVESDGFAMGFAEKIIVDSQNVVPFEGVSFQEASLVEPMGVALDLSYTVKP
                     N"
     gene            1720..2220
                     /locus_tag="PLD15_07835"
     CDS             1720..2220
                     /locus_tag="PLD15_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPB63855.1"
                     /translation="MATQIAKAMGARHVFAANHSRSKIRIDVARQLGVDDVIEVDKTP
                     ISSYKFPRGGVDKALVTAPPYVIPEVLGVMNYGGVVGFIGIDYGEGRFITFDANAFHF
                     NKLQLRASHAVPALYFPACLDMIKSGAVNVKPLITDTFTLEDIERMMKRAEKEKDRVI
                     KAVMIG"
     gene            complement(2207..2869)
                     /locus_tag="PLD15_07840"
     CDS             complement(2207..2869)
                     /locus_tag="PLD15_07840"
                     /inference="COORDINATES: protein motif:HMM:NF021285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPB63856.1"
                     /translation="MILPYHFERTPAGYRSGQLFLDPRTDVLRDRFENAVVDALTCLE
                     YLRATCPPPYYLMGYSFGGFIAVITAAIDGGISGLSLTVTGGNFYHITWKSFVTGVMR
                     VKYEEDGSCNRDKCLEMHGKLFHDYAASLEGPEVGLGSAPISCLEYDPLTYARFVRCP
                     TLLTGALFDIFIPRKSTLELSRAIPDCKVKWLPSGHLSSILFKRRVLREAATFFVNIS
                     RS"
     gene            3197..4378
                     /locus_tag="PLD15_07845"
     CDS             3197..4378
                     /locus_tag="PLD15_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HPB63857.1"
                     /translation="MNTLAVVIAIVVLIVSLRLFKDISLSLLFSVLAIGLILLIHPGN
                     YLQAFTTEIIDWNFWKVIITVFSIYLLGETMDKSGNSTRFVRAVEKLFPEPRVSISLM
                     PAIIGLLPMPGGAMFSAPMVRDLARSDSSITDEDAMVFNYWFRHAMEFFWPLYPALVI
                     AAGISATSINKLVIWLLPVGLVALVSGYLLMIRRPIRIRYSNYAIKELLISAWPIIAV
                     IVLVILNQPGWLAVMGTALIYLLLNKNKKKVIVAALKYKTFILLLTVFFYKNLVDIAG
                     IPQAMGNELIAWSIPPITLVILLPFLMGFMTGVTQAGVGLSLPLILSMGNGYGTLSTV
                     ILAYTFAVVGVLVSPVHLCTVLTVEYFKTEYTRVMKRISVVTLLSIAASIVVFLLPKS
                     T"
     gene            4449..5576
                     /locus_tag="PLD15_07850"
     CDS             4449..5576
                     /locus_tag="PLD15_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63858.1"
                     /translation="MKDYSVVLTNVTKIFDNEFTAVDNVSLKIEQGEFFSLLGPSGCG
                     KTTILRMIAGFEDPTSGNITLNGKNIIGTPPNEREGNTVFQNYALFPHLSVFENIAFS
                     LRLRKEKEGEIRRKVMNMIDLTKLNGHVDKMPSQLSGGQKQRVAIARALVGKPTVLLL
                     DEPLAALDAKLRQHMLVELDTIHDEVGITFIYVTHDQSEAMSISDHVAVMNEGEIIQY
                     GTPFEVYESPVNAFVANFIGETNFFTGTVEKVEEEYFMLDTGRFGKIWFYKDMEVKIG
                     QEIQVSLRPEKVKVTKEKPIAPQGVKINILEGIVDETIYLGSQTKYTVAVGDHYLQAF
                     KQHVRYLLDEVIITWKDRVYVWWFADDSYVLREPGGVPVEE"
     gene            5566..>6212
                     /locus_tag="PLD15_07855"
     CDS             5566..>6212
                     /locus_tag="PLD15_07855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB63859.1"
                     /translation="MKSKRDFTGFLVSMPGILWLTVFFLIPYIIIIIYSFLTSGIYGG
                     VELPFTLEAYSKMLINAGYWQILWRTVWISMIATLICVGLGLPAAYFIATSKHRNMFL
                     TLVIIPFWTNFLVRIFGWMVILGRNGVINSFLQLLGLKEPLNLMYTPGAVILVIVYMY
                     LPYMILPLYSAIEKFDFRLIEAAMDLGAKRTQAIWKVLIPSIRGGIGAGVILGFIP"
BASE COUNT         1594 a         1520 c         1603 g         1495 t
ORIGIN      
        1 cattataccg acaaagacgc tgaaggtccc cgggagatag gctatctctt cgatacagcc
       61 aatgggcaat ctttttgagc agtcgcttct catcctcacc gacatagtag tgatgagact
      121 catggaaaag ctcggcatgg attcgaaaga aatgttcaag aaccacgcca accttgaata
      181 aggaggtagc gaaattgaaa tacagaaaag tgaaaaagat agaagagaaa ttatcgaccc
      241 tcggttacgg ttgctggggt atttccggcc catctttctg ggatggaaca accgacgatg
      301 attcgatcaa gacgatacgc aaagccgtgg gcgagggcat aaacttcttc gatgtggccc
      361 cggtttacgg attcggtcac gccgaagagg ttcttggaaa ggccatcaag ggtatgagga
      421 acgatatctt catcgcgacc aagtgtggcc tcgtctggga tcaacagaac aggataagta
      481 actgcctgaa gccggaaagt atcaggcgcg agatagaggc cagcctgagg agactccaaa
      541 ctgaccgcgt ggatctttac cagcttcact ggcccgatcc ccgggtcgat atagaagaga
      601 cgatggatgc ggtcaatgaa cttaaaaagg ccggtatgat aaggtacata ggcctgtcga
      661 acttctccct gtcccttgca gaaagggcga tgaaagtctc ggatatcgcc agtatgcagg
      721 gactgtacaa catgctcgaa aggaacccca aaagttacca caacatacct ctcgaatacg
      781 cggtggaaaa agaggtcctt ccattcgtaa aagaaaacgg gatggccttt ttaccttata
      841 gtccgctctt tcagggactc ctgaccggga ctttcgatct ggagcgggaa aacttcagtt
      901 cgagcgacgt gagatcggcc aaccccaaat tgagcggccc ggatttcgag aagtattatt
      961 cagtagtgaa ggaactcaac gcaatttccg gggagatcgg taagcctctg agtcagatcg
     1021 ccatcaactg gctgctctac cacaaagaag ttacctcgat cattgccggc gcgcagaaca
     1081 tagcccagct cgagcagaac ctcggggccg ttgaatggga gatcgacaac gggctttacg
     1141 gcaggatcga gacgctggta aagaactcgg gaattgatct gtgaggtgtc aaatgaaagc
     1201 cgcctatgtg aaagctccct tccagtttca gataagagaa gtcgaactcc ccgaaccggg
     1261 gccgaatgac gttctgcttt cgataaaggc ttccggaatc tgcgggaccg acctccatat
     1321 agcccgaact gaggccaggg actggcagcc cttcggccac gagatcgtcg gcttggtcga
     1381 aaagataggt tccaacgtcg gaaatgtttc gcttggcgat aaagtggtgg tcgagagcgg
     1441 ttctttttgc ggtaagtgcg ccgactgcag aaacggtcgc gtagatctct gccacgacag
     1501 aacaccgaac gttttcagca gggtcgagag cgatggtttc gctatgggtt tcgccgagaa
     1561 gataatagtc gacagtcaaa atgtcgtccc cttcgaggga gtttctttcc aggaggcctc
     1621 tctcgtagaa ccgatgggag tcgctctgga tttgagctat acggttaagc cgaactgaac
     1681 gacgatgtgc tggtcgtcgg tcttggaccg atcggcctga tggctactca gatcgcgaag
     1741 gccatgggcg ccagacacgt cttcgcagcc aaccactctc gttccaaaat caggatcgat
     1801 gtcgccaggc agctcggagt ggacgacgtg atagaggtgg ataaaacgcc cataagttcg
     1861 tacaaattcc cccgcggcgg ggtggacaag gcacttgtga cagcccctcc gtacgttata
     1921 cccgaagtgc tgggagtcat gaactatgga ggtgtcgttg ggttcatcgg tatcgattac
     1981 ggcgagggcc gattcataac tttcgacgcc aacgccttcc atttcaacaa actccagctc
     2041 agagcctcgc acgccgtgcc cgccctttac ttccccgcct gtctagatat gatcaagagt
     2101 ggcgcggtga acgtgaagcc cctgatcacc gacactttca cactcgaaga tatagagagg
     2161 atgatgaaga gggccgaaaa ggaaaaggac agagtaatca aagcagttat gatcggctga
     2221 tattaacaaa aaaagtggct gcctcccgca gtacccgtct tttgaaaagt atggaagaca
     2281 ggtgccctga gggcagccac ttaactttgc aatcgggtat cgctcgcgat agttcaaggg
     2341 tcgatttcct gggaatgaag atgtcaaaca gtgcaccggt gagcagtgtg gggcatctca
     2401 cgaacctggc ataagtgaga ggatcgtatt caaggcaaga tatgggggcg ctccccagcc
     2461 ccacttcggg accctcgaga gaagcggcgt agtcgtgaaa gagttttccg tgcatttcca
     2521 ggcacttatc tctgttgcag gaaccatcct cttcgtattt aactctcatg actcccgtaa
     2581 cgaagctctt ccaggttatg tgataaaaat ttcctcccgt aaccgtcagt gaaagcccgg
     2641 agatgcctcc gtcgatagcg gccgttatca cggcgatgaa ccccccgaaa ctgtaaccca
     2701 taaggtaata cgggggagga caggtcgctc ttagatactc cagacaggtc agggcatcca
     2761 ccacggcgtt ctcgaatctg tcgcgcagta cgtcggtgcg gggatcaaga aacaactggc
     2821 ccgacctgta tccggccggt gttctctcga aatggtaagg caggatcatc atcacacccg
     2881 aaaggccgta tctgccgaaa ttctcgggaa accagcgcag gtactcgaga ttcctatctc
     2941 ccctgccatg aagaaagaga acggttccct tctcgccttc tctgtttata taaagaggga
     3001 tttgaatgtt ctcacgaaag tcgcacggga agtttgacct aaggacgaca cgttcgtttc
     3061 ttcccgaacg ggctttctcg acaatcgaag gctctctgga ataacgtttg gcgaaaatct
     3121 cttttttgat tctatatcac ctcaattaat tatataatag tgcgataagc taaccgtgct
     3181 ttgttaggag gccgatatga acactctcgc ggttgtaatt gccatcgtcg ttcttattgt
     3241 atcgctgagg cttttcaaag acatttctct gtctctgctc ttttcggttc tcgccatagg
     3301 gttgatactc ctgatccatc cgggtaatta tctccaggcc ttcaccacag agataattga
     3361 ttggaacttc tggaaggtaa tcatcaccgt cttttctatt tacctacttg gcgagactat
     3421 ggacaagagc gggaattcga ccaggttcgt gagagccgtc gaaaaactct ttccagaacc
     3481 cagggtttcg atatcgttga tgcctgcaat catcggttta ctccccatgc ccggaggcgc
     3541 gatgttctcg gcgcccatgg tgagagatct cgccaggagc gattcctcta tcaccgacga
     3601 agatgcgatg gttttcaact actggttcag acacgccatg gaattcttct ggccgctcta
     3661 tcccgctctg gtcatagcgg ccggtatatc ggcaaccagt ataaacaagc tggttatctg
     3721 gcttcttccg gtgggccttg tcgcgctcgt gagcggctac cttctgatga taaggagacc
     3781 cataagaatc aggtacagca attacgctat caaagagctc ctgataagcg cctggcctat
     3841 tatcgcggtt atcgttctgg taatactgaa ccagccgggc tggctggcgg ttatgggaac
     3901 ggccctgata tatctgttgc tgaacaagaa caagaaaaag gttatagtcg ctgcgctcaa
     3961 atacaagacc tttatacttc ttttaacggt ctttttttac aagaatctgg tcgatattgc
     4021 agggataccg caggcgatgg gcaatgaatt gatagcctgg tcgatacccc ccatcactct
     4081 ggtgattctg ttgccgtttc tcatgggttt catgactggc gtcacacagg ccggtgtcgg
     4141 cttgagtctc cctctcatac tcagtatggg taacggttat ggtacactct ctactgttat
     4201 actggcgtac acgttcgcgg tcgtgggggt cctggtctct cccgtccatc tttgcaccgt
     4261 gctcactgtg gaatacttca aaactgaata cactagagtc atgaagagga tatccgtggt
     4321 cacgctcctc tcaattgcgg caagcatagt ggtttttctg ctacccaaat caacttgaca
     4381 ttaacacata gggagaccgc cacggggtat acttctactt actgatctcg attggaggtg
     4441 agaaggaatt gaaagattac tccgttgtcc ttaccaatgt cacaaagata ttcgacaatg
     4501 aattcaccgc tgtggacaac gtttctttga aaatagaaca gggcgagttt ttttctcttc
     4561 tgggaccctc tggttgtggc aagactacca tcctcaggat gatagccggc ttcgaagatc
     4621 caacttccgg caacataact ctgaacggta aaaatattat cggtacacct ccaaatgaaa
     4681 gggaagggaa cacggttttc caaaactacg ctctctttcc ccatctgagc gtcttcgaaa
     4741 atatagcttt cagtctcagg ttgagaaagg agaaagaggg agagatccgg cggaaggtca
     4801 tgaacatgat cgatttgact aagctgaatg gccacgtcga taagatgccc tcgcagcttt
     4861 caggaggaca gaagcagagg gtggcgatcg ccagagcgct tgtgggaaag cccaccgtgc
     4921 ttcttctgga cgaaccgctg gcggcgctgg acgccaaact gagacagcat atgctcgtcg
     4981 agctcgacac gatccacgac gaagtgggaa taaccttcat ctatgtaact cacgatcaaa
     5041 gcgaggctat gtcgatatcc gatcacgtgg cggtgatgaa cgaaggcgaa attattcagt
     5101 acggaacgcc cttcgaagtc tatgagagtc cggtcaatgc cttcgtcgcc aattttattg
     5161 gagaaaccaa tttcttcacc ggaaccgtcg aaaaggtaga agaggaatac ttcatgctcg
     5221 acacaggccg gttcggaaag atctggtttt acaaagatat ggaagtcaag atcggtcaag
     5281 agatacaggt ttcgctacgt ccggaaaaag tcaaagtgac taaagaaaag cccatcgccc
     5341 cgcaaggtgt caagataaat atactcgaag gaatcgtaga tgagactata tatctcggaa
     5401 gtcagaccaa gtacaccgta gcggtgggcg atcattatct acaggccttc aagcaacacg
     5461 tgagatattt gctggacgag gtgataatta cctggaagga ccgcgtgtac gtctggtggt
     5521 tcgccgacga cagttacgtc ctgcgcgaac ctggtggtgt ccccgttgaa gagtaagaga
     5581 gatttcacgg gttttctggt ttcgatgccc gggatactct ggctgaccgt ctttttcctg
     5641 atcccgtaca tcataatcat aatctacagc tttctgacat cgggaatcta cggcggcgtg
     5701 gaacttccgt tcactctcga ggcctactcg aagatgctga tcaacgccgg ttactggcag
     5761 atactctgga gaaccgtctg gatatccatg atcgctacgt tgatctgcgt ggggcttggt
     5821 cttccggcag cttatttcat tgccacgtca aaacacagaa atatgtttct cactctagtc
     5881 atcataccct tctggacgaa ttttctggtc agaatcttcg gctggatggt catactggga
     5941 aggaatggcg tgatcaactc ctttttacag ctacttggcc tgaaagagcc cttgaatctt
     6001 atgtatacac ccggggccgt gatactggtg atcgtgtaca tgtaccttcc gtacatgata
     6061 ctaccgcttt acagcgcgat agagaaattc gatttcagac tcatagaagc ggccatggac
     6121 ctgggagcta aaagaaccca ggccatctgg aaagtcctca taccttcgat tagaggtgga
     6181 ataggagctg gagtcatact gggtttcata cc
//
LOCUS       DAOOLY010000144         6140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15269_length_6140_cov_4.739688, whole genome shotgun sequence.
ACCESSION   DAOOLY010000144 DAOOLY010000000
VERSION     DAOOLY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15269_length_6140_cov_4.739688"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLD15_07860"
     CDS             <1..995
                     /locus_tag="PLD15_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HPB63860.1"
                     /translation="VSGFEEKVASFIEKEIEGKVDELWKDSVGNLMAIKKGNGKSSRR
                     LMLLAHTDEVGLMVKRINEDGSLSFTAIGGVDPRVLMGKKVFVGEEMLPGVIGFEAIH
                     TQESSTLLKTPSMDKLRIYLGYSKKEEAQKSHRIGDPVFFSTAYEETGEYAVAKAFDD
                     RTGCEVLLRVLDDVQGLSMDYDLCFAWVVQEEVGLRGSGIAARQVKPDAALVFENTTA
                     GDNPELPEYRWATNLGMGPVLTFAHSGLVLDRRIFDTIVETARRNSIKFQYKRRIAGG
                     TDAGRLARTMSGIPAGVISTPSRYIHSPTSIININDFLGVAELASVLVKEGKVL"
     gene            996..2003
                     /locus_tag="PLD15_07865"
     CDS             996..2003
                     /locus_tag="PLD15_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB63861.1"
                     /translation="MKELIKKLVEISSPSGREDAIREAIAEEVRDYVDELRVDALGNL
                     ICMKRGKSGKKLLLDAHMDEIGVVVTHIDEKGFLRIDMLGGVSPYMLLGSRIIFESGA
                     CGIVSTEGESAREAGEIMKNLDFDHIFVDIGASSKEEAEKLAPIGTFGTYDSKFRDLG
                     EKLVSKSMDDRIACAILVQTIKELKEPADDVYFVFAVQEEVGIVGASVAAFDIMPDMA
                     VAVDVTAGPDTPKSFKRMGFKLGAGPTIKIKDRGSVSSSRVVSRLKEVADIRSIPVQY
                     EVLIFGGTDARGYQITGKGIAAGTVSVPTRYVHSPNEVVDYNDVLNTVRLLKALAEEG
                     V"
     gene            2012..3001
                     /locus_tag="PLD15_07870"
     CDS             2012..3001
                     /locus_tag="PLD15_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPB63862.1"
                     /translation="MKRVAFTSESSSNCQLIYGEKLLERLPESLKILDSGARKALQIH
                     PEDEYLMPGGEHIKSIDRVLEVYQVLRTSGRRAITVIGGGSLIDLVGYAASTHGEVDR
                     LFLYPTTTVSQVMPPLSGFSINFEFVKNLLNSSGLPDRIYVDTELTYGTMFQIRRGEI
                     FFPLLVAYSLDERLFKYILNHIVSGGEVTRELWGDIIYSASSAFVKSLGSSRMVVGCG
                     VGDLIQSASRLRTEYSTALIYGAILELHIARTSGLIDGAGKEDLYEALRVLGKPDRSI
                     KIDVSSLVDLVNQKGGVKIALPSRSGESSHWVTARDFETQVRDRPWMGFGSIL"
     gene            2998..3738
                     /locus_tag="PLD15_07875"
     CDS             2998..3738
                     /locus_tag="PLD15_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HPB63863.1"
                     /translation="MSGESQDIVVKIMIVIVLALSGIVFTLAGYAILLRQENKKYDLI
                     IEEIRSSVLPDLSGGATPLELPPISQGVTEVPRDVQNIPVEQHFFLETFDYRKLILNS
                     FDFLVEGSGFNFVVVEQDVAFKLCVEMGQGKYFITRVNDMYGVMFIGDNPLPGLYESK
                     TAYGIQLVSHTISDGIAPQVIDLRSEGYPACVYKSVTTDGRTFYAAVLGIFPDAAAAR
                     EYSNSLDVQSLQRITGWNITDRFPRQLR"
     gene            3735..4511
                     /locus_tag="PLD15_07880"
     CDS             3735..4511
                     /locus_tag="PLD15_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HPB63864.1"
                     /translation="MRKLSIKEIQIIGSVAVLVLIGFVAVSGSLKSVPDTVNTPEAGK
                     TVTFPLDLNISTEEELDLLPGIGPSKAKAIVEYRKASGGFNDVEELLNVKGIGAKTLD
                     KLKPLIKVGESSGRETKAKASPESIDINTASLEELKEIPGIGDSKAKAIIDHREKNGK
                     FVSVSDLLSVPGIGPSNIEVILEKIKPLEVAMAGSGKINVNTASESELDRLPGIGQVL
                     ASRIVEYRKSNGRFTKCEELLEVKGIGESILKDIEELIEF"
     gene            4508..5308
                     /locus_tag="PLD15_07885"
     CDS             4508..5308
                     /locus_tag="PLD15_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPB63865.1"
                     /translation="MTLIHVCCAPDLLSTVLKREFPEAVFYFYNPNIFPESEYEKRLE
                     AFKTVCHRLNLNYMEGRYDPEEFSAFFERYFDEEEGGARCGKCMEMRLKNAALTSGKI
                     HAKSFTSTLLASPRKSIDTINEIGMEISNVYGTEYTGGNFRTGRKEIKPYLEDIYVQN
                     YCGCEASLVKSRRDREERERMDFELLSGKFRNLVHLWKYRGRLIRIEEIPIRDESSLK
                     EFLAVLKPSGLLVKKGSELYGRNWLKAGKYNCRILGESDIYGESGQKN"
     gene            5283..5591
                     /locus_tag="PLD15_07890"
     CDS             5283..5591
                     /locus_tag="PLD15_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63866.1"
                     /translation="MENLAKKIRELGETCGMVFRIVDKNGKSLEEGYDLEEIPPLVIA
                     LSKATGSRSSTLSGGVPLTAVYLDGMRNECYLIVVGEFREDNAFRLLKNIAEAFEVRL
                     "
     gene            5588..>6140
                     /locus_tag="PLD15_07895"
     CDS             5588..>6140
                     /locus_tag="PLD15_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase, N-terminal
                     domain-containing protein"
                     /protein_id="HPB63867.1"
                     /translation="MTKFCLRIDKPLDLDATLDCGQTFRWTARDGWWTGIVRNTVIRL
                     KQERNELRVKASSDSLLGRDIDRGLISYLGLEDNLEEIHIDIEKKLASMNSKVSDISK
                     KALEEARGLRILRQDPFEMVVEFILSTRNNIPTIRKMSDGLSAIFAENRVEFEGETFF
                     LFPDLEQARRIGQQQLEELKIAFR"
BASE COUNT         1682 a         1363 c         1725 g         1370 t
ORIGIN      
        1 gggtttcggg atttgaagaa aaagttgcct ccttcatcga gaaagagata gagggaaagg
       61 tcgatgagct ctggaaagat tcggtgggca acctcatggc gataaagaaa gggaacggca
      121 aaagctccag aagattgatg cttctggccc acaccgatga agtcggcctg atggtcaaga
      181 ggatcaacga agatggatcc ctatctttca ccgccatagg cggtgtcgat ccgagggtgc
      241 tgatgggaaa gaaggtcttc gtaggcgagg agatgctgcc gggcgtgata ggcttcgagg
      301 cgatccacac acaggaaagt tcgacgctgt tgaaaacccc gtccatggac aaactgcgta
      361 tctatctcgg ctattcgaag aaggaagagg cccagaagag tcacaggatc ggggatccgg
      421 tcttcttttc cactgcttac gaagagaccg gagaatacgc tgtggccaaa gccttcgacg
      481 acagaaccgg ttgcgaggtc cttcttagag ttctggacga tgtacagggc ctctctatgg
      541 attacgatct ctgtttcgcc tgggtcgttc aggaagaggt tgggctcaga gggagcggaa
      601 tagccgccag gcaggtcaag cccgacgcgg cactcgtttt cgagaacacc accgccggag
      661 acaaccccga actcccggag tatcgatggg ccacaaacct cggtatggga ccggttctca
      721 cctttgcgca cagtggcctc gttctggaca gaaggatctt cgacaccatc gtcgagacag
      781 ccagaagaaa ttcgatcaaa ttccagtaca aaaggcggat agccggaggt acagacgccg
      841 ggcgtctagc gagaaccatg tcggggattc ctgccggtgt catttcgacc ccgtcaagat
      901 acatccattc tccgacttcg ataataaaca taaacgactt tcttggagta gcagagcttg
      961 ccagtgttct ggtaaaagaa gggaaggtgc tttagatgaa ggaattgata aagaaactag
     1021 ttgagataag ttcccccagc ggaagagaag atgcgataag agaagccatc gcggaggaag
     1081 taagagatta cgtcgatgaa ctgagggtcg atgcactggg aaacctgatt tgcatgaaaa
     1141 gaggaaagag tggaaagaag ctgcttctgg acgcccatat ggacgagatc ggagtggtgg
     1201 tgacccatat cgacgagaaa ggcttcttga gaatcgatat gctcggaggc gtttcaccgt
     1261 acatgcttct gggttcgagg ataatttttg aaagcggcgc ctgcggtatt gtaagcaccg
     1321 aaggtgaatc ggccagagaa gctggcgaga taatgaagaa cctcgacttc gatcacattt
     1381 tcgtagatat cggagcctcc agcaaagaag aagccgaaaa gctggctccg atcggtacct
     1441 ttggcacgta cgattcgaaa ttcagagatc taggtgaaaa actcgtttca aaatctatgg
     1501 acgacaggat agcctgcgcc attctggtgc agacgataaa agaactcaaa gagccggccg
     1561 acgatgttta cttcgtcttc gccgttcagg aagaggtggg aatagtcggg gccagcgttg
     1621 ccgcgttcga cataatgccg gatatggctg ttgcggtcga tgtaacggcc ggaccggata
     1681 caccaaaatc cttcaagaga atgggattta agctgggggc tggacctacg ataaagataa
     1741 aggatcgcgg aagcgtcagc tcctccagag tggtatccag attgaaggaa gtggcggata
     1801 tacgttctat tcctgtccag tacgaggttc ttatcttcgg cggtacggac gccaggggat
     1861 accagatcac cgggaagggc atcgcggccg gaacggtttc cgtaccgacc agatacgtcc
     1921 attctcccaa cgaagtggtg gattacaacg atgttctcaa cacggtccgt ctcttgaaag
     1981 cgctagccga agaaggagtc tagagaaggc aatgaaaaga gtcgctttca ccagtgaatc
     2041 ttcgagcaat tgtcagctga tttacggtga aaagttgctg gaaagactgc cggagtctct
     2101 gaaaatactc gattccggtg cacgcaaggc tcttcaaata catcccgaag atgagtacct
     2161 gatgcccggc ggagagcaca taaagtctat agatcgtgtt ctagaagtct atcaggtact
     2221 caggacctcg gggaggaggg cgataaccgt cataggcggg ggctcgctca tagacctggt
     2281 cggttacgcg gcatccacgc acggcgaggt cgacagactc tttctgtatc cgacgaccac
     2341 cgtcagtcaa gttatgcccc cgctttcggg tttcagtata aacttcgaat tcgtcaagaa
     2401 cctgttgaac tcgagcggac tccccgacag aatatacgtg gataccgagc tgacgtacgg
     2461 caccatgttt cagatacgca gaggggagat ctttttcccg ttgctggtgg cttactcgct
     2521 ggatgagcgc ctgttcaaat atatcctgaa ccatatcgtc tccggtggcg aggtgacccg
     2581 cgaactttgg ggcgacatca tctactcggc atcttcagcc tttgttaaat cgctgggcag
     2641 ttccaggatg gttgtcggat gcggggttgg cgatctaatt caatcggctt caagactgag
     2701 aacagaatac tccaccgccc tgatttacgg agccatattg gaactacaca tcgcgagaac
     2761 ctccgggctt attgatggag cggggaagga agacctttac gaggcgttga gagttcttgg
     2821 gaaacccgac cggtccatta agatcgatgt cagctctctg gtcgatctgg tcaatcagaa
     2881 gggcggagtg aagatcgccc tgccttccag gtcgggtgaa agctctcact gggtaacggc
     2941 aagggatttt gaaacgcagg tcagggatag accctggatg ggatttggga gtattttatg
     3001 agcggagaat cacaggatat agtcgtcaaa ataatgattg taatcgtgct tgccctctcc
     3061 ggcatagtct tcacgcttgc cggttacgcg atacttctca ggcaagagaa caaaaagtac
     3121 gatctcataa tcgaagagat aagaagttcg gtccttcccg atctgtcagg cggggcgacc
     3181 cccttagagc tcccgccgat ctcacagggt gtgaccgaag taccgcgcga tgttcaaaac
     3241 atcccggtgg aacagcattt ctttctcgaa accttcgact acaggaaact cattctaaat
     3301 tcgttcgatt ttcttgtcga gggatcgggt ttcaattttg ttgtggttga acaggatgta
     3361 gctttcaagt tgtgtgtcga gatgggtcag gggaagtatt tcatcaccag ggtgaacgac
     3421 atgtatgggg taatgtttat aggcgacaac ccactccccg gactgtacga atcgaagact
     3481 gcctacggta tccagctggt ctctcatacg atttccgacg gaatagcgcc acaggtaatt
     3541 gacctgagga gcgaaggata tcccgcatgt gtctataaat ccgtcaccac agacgggaga
     3601 actttctacg ctgccgtgct gggaatcttc cccgatgcgg cggccgctag agagtactcc
     3661 aattctctgg atgtacaatc acttcagaga ataactggct ggaatattac cgataggttt
     3721 cccagacagt tgagatgaga aagttatcga taaaagagat tcagatcata ggttccgtag
     3781 ccgttcttgt tttaatagga tttgtagccg tttcgggcag tctcaagtcc gttcccgata
     3841 cggtgaatac gccggaagct ggtaagaccg tgaccttccc gctggatctc aatatatcga
     3901 ccgaagagga actggatttg ctcccgggta taggaccctc gaaggcgaaa gcaatcgtgg
     3961 aatacagaaa agccagcgga gggttcaacg acgtggaaga gttgttgaac gttaagggga
     4021 taggtgctaa aacgctcgat aagctgaaac ctttgataaa agtcggcgaa agctccggga
     4081 gagagacgaa ggcgaaggcc tcacctgaaa gcatcgacat aaacacggcc tcgctcgaag
     4141 agctcaagga gatcccggga atcggtgatt ccaaggcaaa agccataatc gatcatcgtg
     4201 aaaagaacgg gaagtttgtg tccgtttccg atcttctctc cgtaccgggt ataggtcctt
     4261 cgaatatcga ggtgattctg gagaaaataa aaccgcttga agtagcaatg gccggttctg
     4321 gaaagataaa cgtaaacact gcgagcgaaa gcgaactcga cagacttcca gggatcggac
     4381 aggtgctggc ctcgagaata gtcgagtaca ggaagagcaa cggccgcttt actaaatgcg
     4441 aggagttgct cgaagtgaag gggataggtg agagtattct gaaggatata gaggagttga
     4501 tagaattttg acgctcatac acgtctgctg cgcgcccgac ctgttgagca cggttttgaa
     4561 aagggagttt ccggaggccg ttttctactt ctacaatccc aacatcttcc ctgaaagtga
     4621 atacgaaaaa cgactcgaag ccttcaaaac cgtctgccat aggttaaacc ttaactacat
     4681 ggagggacgg tacgatccgg aagagttttc agcgtttttc gagagatact tcgatgaaga
     4741 agaaggtgga gccagatgtg gaaagtgcat ggagatgagg cttaaaaacg ccgccctgac
     4801 atcgggaaag atccatgcca aaagcttcac cagtactttg cttgccagtc ccagaaagtc
     4861 tatagacacg ataaatgaga ttggaatgga aattagcaac gtttatggaa ccgaatatac
     4921 aggcggcaat ttcagaaccg gcagaaaaga gataaaaccg tatctcgagg acatctacgt
     4981 tcagaattat tgcggttgtg aggccagtct tgtaaaatcc agaagggaca gggaagagcg
     5041 agaaagaatg gattttgaac tcctctccgg aaagttccgg aatctcgttc acctgtggaa
     5101 atacaggggt aggttaataa ggatcgaaga gatacccata agagatgaaa gctctctgaa
     5161 ggagtttctg gccgttctaa aaccctccgg tctgttggtg aagaaaggat cggagctgta
     5221 cggaaggaac tggctgaaag cgggaaaata caattgcagg atactcgggg agtctgatat
     5281 atatggagaa tctggccaaa aaaattagag agcttggaga gacctgcggg atggtctttc
     5341 gaatcgtcga taagaacggg aaatccctcg aagagggtta cgatctcgag gagataccac
     5401 ctctggttat cgcgctttcg aaagccaccg gttcgaggag ttcgaccttg agcggtggtg
     5461 tgccgttaac cgccgtttat ctggatggaa tgcgaaacga gtgctacctt atagttgtgg
     5521 gcgaattccg tgaagacaac gccttccggt tgctcaaaaa catcgcggag gctttcgagg
     5581 tgagattatg acgaagttct gtttgagaat agataaacca ctggatcttg atgccacgct
     5641 agattgcggg cagacttttc gatggaccgc cagagatggc tggtggacag gaatcgtcag
     5701 gaacaccgtg atccggctga agcaagaaag gaacgaactc agagttaagg cctcttcgga
     5761 tagccttttg ggaagggata tagaccgtgg attgatctct tatctcggac ttgaagacaa
     5821 tcttgaagag atacacatcg atatcgagaa gaagctcgct tcgatgaatt cgaaagtttc
     5881 ggatatatct aaaaaggcgc tcgaagaggc gcggggactg agaatactca ggcaggaccc
     5941 cttcgagatg gtggtcgagt tcatactttc cacaagaaac aacatcccca caatcagaaa
     6001 gatgagcgat ggtctatcgg cgatcttcgc tgaaaacagg gtggagtttg aaggggagac
     6061 cttcttcctc ttccctgatc tggagcaggc cagacgtatc ggacagcaac agctcgaaga
     6121 gctgaaaatc gccttcagag
//
LOCUS       DAOOLY010000145         6139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15271_length_6139_cov_4.621631, whole genome shotgun sequence.
ACCESSION   DAOOLY010000145 DAOOLY010000000
VERSION     DAOOLY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15271_length_6139_cov_4.621631"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLD15_07900"
     CDS             complement(<1..369)
                     /locus_tag="PLD15_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63868.1"
                     /translation="MHRRYVVILAVLVSTVFLLAVPFAGSKIENIASARFIDGDGNGI
                     TIFSEKVVTTVLPVYGFIVKPDGTFANPGQIESGKAGATVDLKNTVTNTGNIETYVYL
                     KAGQVFETDLEEETFFKVASS"
     gene            complement(460..2952)
                     /locus_tag="PLD15_07905"
     CDS             complement(460..2952)
                     /locus_tag="PLD15_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63869.1"
                     /translation="MGRKFLVILIAVTIAVAAIAVPPAAGTKIKNQAAATYLDNRGVT
                     RTVLSNQVITTVLPIYSVLVTPDLIDAPGSAGQTMQFQFRVYNLANTSDSFNTSVRID
                     DASTFIPQNIEVYCDENGNGVVDPGEELWKNTTSIPSGEFRYAIVRYRVPSAAAAETT
                     ATVYLDVDSVNDPVNAFDHDNAARVTIYNDAVVTIFKSATPQEVKARDEVTYLLSGSN
                     SGTKAAANVTITDNLPSSLEYLGFQDKVPDGMTVNVVGNTVTLHIANLNAGSSYQVRL
                     RARVKADTLAGIVENVASMSYVISTGATVTRNSNVSTIMVGGSGYETVKVWIGPKDAP
                     EAVDPNDITIKPGVAGTLVEFVNTVKNAGLSTDIINVTMAGVVPAELTNVVAISFFAE
                     NLIPLPDSDDNGMQDTGPLAPGETFDVTVKIYIPSDISELIDSITATVRAISSISETA
                     SDTTIDKVEPITFPDVAIGNATGTAFDTEVDKNPVNMNGDPGSYVYFPLDVANKSDAA
                     DTFSLSASVPAGYVVRFYIDADGDGILDPEEMIPVTNTGQVNGNSGIRIIARVMIPEG
                     TLYTGTAVPVEFFATSLTRSSVSDSQINTITVNAVRSIVLQPSRNGSAAPGQNIDYEH
                     TLTNNGNIAVTVKLYPSSMRNWAFTFLSGGVPVPLDATFVLQPGESISGIFRLEVPSD
                     EPLGVTDVAYLKAEVQEDTTVTSTVIDITVVVSANVTITKAADKLEAQPGEQIIYTIT
                     YADVGTEAINDLIIYDTIPFYTSLDPVIVTDTVNFTEPSQVSKDYGVTWEDWVTFNKA
                     DIALITTVKWIIGNLTSGQSGTVVFPVLIDGY"
     gene            complement(2975..3484)
                     /locus_tag="PLD15_07910"
     CDS             complement(2975..3484)
                     /locus_tag="PLD15_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63870.1"
                     /translation="MKKIVLFIVSLVLAVSALGATEEPVNVSIESYKIWDSQTATVTY
                     EPLEEVFPGDEILYVLTYENLSDIDIEGLQMVAMIPEGTFYIKGSATGEKREHATWEA
                     TGLELLFSYDGGNVFAKSPLFVEEVKGGMTVKRLVNPMDYTNIMWICNARFKPSDILK
                     VLYKVKVLS"
     gene            complement(3498..>6139)
                     /locus_tag="PLD15_07915"
     CDS             complement(3498..>6139)
                     /locus_tag="PLD15_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPB63871.1"
                     /translation="GAVVNSNQAVFGGGTAGVENVPVAQIQGKEIEINCNDLSHFVRT
                     ATGGIDFLLKNMAFFNGGGNLGDSFAPVILNGPGFLLESIVTGARANGTRPTDVLLNR
                     RYGLIINSSGTLSNSYIAHNGSGLLLTGSDLTVTGVHVLDNGIGVAGTDGNGISLAAP
                     AGPVTISNSIIDSNGGTHAGVNHGNGIYTSGDTSTTLENLTVSGSTASGLSFNSSNLP
                     SVKKSIVRGSASGPGIRVSSDSKFGDFSMNSYYSNKGLAIDLLRSLTAVIIEGVNPND
                     GVLNPDYGNDGVDHPVISLASKSGNTLSVSGYVGQNAGSPVFQGAVVEIYAATAGEGD
                     SYGGLEYGEGATYLGELPVDAQGNFEGSLSAGSLEIVALTAITRYESFSDSLGSFAYS
                     TSEFGPNKPIGAGLKITGYLFEDLNFNRFKDSGEPGINSVRIELWKLEGTEWTLQTYT
                     LSDSGGFYSFNVSQGSFRVVEDALDLYNSSTGGSDPSGYISTTPNYIEVMVDTSNLEV
                     NFGDFRGFTVTGYVFDDSGGGVPSQANNALKDPGEKGIAGARVTLTNGSQIYTRYTDQ
                     AGLYRFFVSGGPVYPLVIRETDMPAYTSTGDHDSDGSASLEERNRITINSGVDFGEYY
                     NFADVKRLLLEGINTVSTLPGLVAYLDHTLIVSTAGNVSITAISARGFGLTVYETDRT
                     GNVLGIWNDASLRLPGEYYIRVAVDVPSDAINGTLDNITIRAVQDWLNSTGEDTAETY
                     DTVSVESGGLTITKATRNPALSGDWGFSSEGKPGDTIEYRITFTNNGLYPLTSIVITD
                     PLAYTLTLLQNCYSLGGSWGNVLLSFDGIDRLLVAEPGGDSNIDGAYLSETTLSIDIT
                     RVVGTLRPGSSGSFIFRTTIREQ"
BASE COUNT         1287 a         1634 c         1667 g         1551 t
ORIGIN      
        1 ggacgacgcg actttgaaga aggtttcctc ttccagatcg gtctcgaaga cctgtccggc
       61 cttcaaatac acataagtct ctatgtttcc cgtattcgtg accgtgttct tcagatcgac
      121 cgtagctcct gcttttcccg actctatctg gccgggattt gcgaaggttc cgtcgggctt
      181 cactatgaag ccgtagacgg gaagcaccgt ggtgacgacc ttctcggaaa agatggtgat
      241 accgttgccg tcgccgtcga tgaaacgggc cgaggctata ttttctattt tcgaaccggc
      301 gaacggtacc gccaggagaa agaccgtgct taccagtacc gcgagtatca ccacatatct
      361 tctgtgcacc tgatcacccc atgttgttac ctctcgtctc tgctatcgtt accgatagca
      421 gagatgaaag gccatggccc ggaggccatg gcttttggat caatatccgt cgatcaagac
      481 cgggaagaca acggtgcccg actgtcccga ggtcagattg cctattatcc atttaacggt
      541 ggtaatcaga gcgatatcgg ctttgttgaa agtaacccag tcttcccagg tcactccgta
      601 gtccttactc acctgggacg gttcggtgaa gtttacggtg tcggttacta ttacagggtc
      661 cagagacgtg tagaagggaa tggtatcgta gattatgagg tcgtttatag cttcggttcc
      721 aacgtctgcg taagttatcg tgtagatgat ctgctctccc ggctgggctt ccagcttgtc
      781 ggccgccttg gttatcgtta cattggccga aacgacaacc gtgatatcga taactgtgga
      841 tgtgacggtc gtgtcctcct gaacctcggc tttgagatag gcgacgtccg taactcccag
      901 aggctcgtcg ctgggtactt ccagcctgaa gatcccggat atgctttcac ccggttgaag
      961 gacaaaggta gcatctagcg ggaccggcac cccgccgctg aggaacgtga aggcccagtt
     1021 cctcatggaa ctcggataca gtttcaccgt aaccgctatg tttccgttgt tagtcagtgt
     1081 gtgctcgtag tctatgtttt gaccaggtgc ggccgagccg tttctggacg gctgaagaac
     1141 tatgctgcga actgcgttga ccgttatggt gttgatctga gaatccgaga ccgacgacct
     1201 ggtgagagag gtcgcgaaga actccacagg gactgctgtt cccgtgtaga gagtcccttc
     1261 gggaatcata acccttgcga tgatcctaat tccgctgttg ccgttgacct gtccggtgtt
     1321 ggtgacggga atcatctctt cgggatcgag gatgccgtcg ccgtcggcgt cgatatagaa
     1381 cctgacaacg tatccggccg gaacggaggc cgagagactg aaggtatcgg ccgcatcaga
     1441 cttgttggcg acgtcgagcg ggaaatagac gtaagagccg ggatctccgt tcatgttcac
     1501 aggattctta tcgacttccg tgtcgaaggc cgttccggtc gcattaccga tggccacgtc
     1561 ggggaaggtt atgggttcga ctttgtctat cgtggtgtcc gaggcagtct ccgatataga
     1621 agagatagcc ctgacggtcg ccgtgataga gtctatgagc tcgcttatgt ccgaaggtat
     1681 gtagatcttc acggtaacgt cgaaggtttc ccctggcgca agcgggcccg tatcctgcat
     1741 gccgttgtcg tcgctgtcgg gaagcggtat gaggttctcc gcgaagaagg atatagcgac
     1801 gacgttggta agttcggccg gaaccacacc ggccatcgtg acgttgatta tgtccgtcga
     1861 gagaccggcg ttcttgacgg tattgacgaa ctccaccagc gtgccggcaa cacccggctt
     1921 gatcgttata tcgttaggat ctacagcttc tggagcatct ttgggaccga tccagacctt
     1981 aacggtctcg tagccggagc cgccgaccat tatcgttgac acgttggagt ttctcgtgac
     2041 ggtcgcgccc gtactgatta cataggacat cgaagcgacg ttctccacga ttcccgcaag
     2101 cgtgtcggcc ttcacgcgcg ctctcaacct cacctgatag ctagaacccg cgttcaggtt
     2161 ggcgatgtgg agagtgaccg tgttgcccac gacgttgacg gtcataccgt ccgggacttt
     2221 gtcctggaag ccgagatact cgagactcga gggcaggtta tcggtgatgg ttacatttgc
     2281 ggcggccttg gtacccgagt tgcttcccga gagaagataa gtcacttcgt ccctggcctt
     2341 tacttcctga ggggtcgcgg atttgaagat agtcacgacg gcgtcgttgt agattgtcac
     2401 tctggcggcg ttgtcgtggt cgaaggcgtt tacagggtca tttaccgagt cgacgtccag
     2461 atagaccgtc gcggttgttt cggccgcggc ggcgctggga accctgtatc tcacgatagc
     2521 gtagcggaac tctcccgatg gaatcgaggt cgtgtttttc cagagttcct ctccgggatc
     2581 gaccacaccg tttccatttt catcgcaata aacttctatg ttctggggga tgaaagtgga
     2641 agcgtcgtcg atcctgacac tcgtgttgaa agagtccgaa gtgttggcca ggttgtagac
     2701 cctgaactgg aactgcatgg tctggccggc cgatccgggt gcatctatca ggtccggtgt
     2761 gaccagtacc gagtagatcg gtagaactgt cgttatcacc tggtttgaaa gtacggttct
     2821 agttactcct ctgttatcca ggtaagttgc ggccgcctgg ttcttgatct tggttccggc
     2881 agccggtggt acggctatcg cggcgacggc tatcgttacc gcgataagga ttacgagaaa
     2941 tttcctgccc ataaagctca cctcccgtgc atttttaact cagtaccttt accttgtaga
     3001 gaactttcaa tatatctgag ggtttgaatc tcgcattgca tatccacatg atattcgtgt
     3061 aatccattgg attcaccagt ctcttgacgg tcattcctcc tttcacctct tcgacgaaca
     3121 acggcgattt ggcaaagacg tttccgccgt cgtagctgaa aagcaactcc agtcctgttg
     3181 cctcccaggt ggcgtgttct ctcttttcac cggtcgcgct ccccttgatg tagaaagttc
     3241 cttcggggat catggcgacc atctgcagac cctctatatc aatgtcagag aggttttcgt
     3301 atgtcagtac gtacagtatc tcgtctccgg ggaagacctc ttcaagaggt tcgtaagtga
     3361 cagtcgccgt ttgagagtcc cagatcttgt aactctcgat actgacgttc accggctcct
     3421 cggttgcgcc aagtgctgaa actgccagca ccaacgagac gataaataaa acgattttct
     3481 tcatttacat tcctccctta ctgctccctt atagttgttc tgaatatgaa tgagcccgac
     3541 gagccgggcc tgagtgttcc aactaccctg gttatatcta tgctaagagt ggtttcactc
     3601 aaatatgcgc cgtcgatgtt tgaatctccc ccgggttcgg ccaccagaag gcgatcgata
     3661 ccgtcgaaag agagtaggac gtttccccat gaaccgccca gcgaataaca gttctgaagc
     3721 agcgtcagag tatatgccag aggatcggtt atcacgatcg atgtgagcgg atagagaccg
     3781 ttgttggtga atgttatcct gtattcgatg gtatctccgg gcttgccttc ggaggaaaat
     3841 ccccaatctc cggacagcgc gggattcctt gtggccttcg tgatcgtcag ccctcccgat
     3901 tcgaccgaga ccgtatcgta agtttcggcg gtatcctcgc cggtggagtt cagccagtcc
     3961 tgcacggccc ttatcgttat gttgtccaga gtgccgttga tcgcatcgct cggcacatcc
     4021 acggcaaccc ttatatagta ttctcccgga agtctgagag atgcatcgtt ccagatgccc
     4081 agcacattgc cggttcggtc ggtttcatat acggtgagtc cgaatcccct ggcggatatg
     4141 gccgtgatcg agacgtttcc ggccgtcgaa acaatgagcg tgtgatcgag gtaggccacc
     4201 agaccgggaa gagtcgagac ggtgtttatc ccctctagga gaagtcgctt cacgtccgcg
     4261 aagttgtaat actctccgaa atcgacaccg gaatttatcg ttatcctgtt gcgttcttcg
     4321 agacttgccg agccgtcgct gtcgtggtct cccgtggatg tgtaggcggg catatccgtc
     4381 tcccttatta ccagaggata tacgggaccg cccgatacga agaacctgta gaggcctgcc
     4441 tgatctgtgt atctcgtgta aatctggctg ccgttagtca gggttaccct ggcgccggct
     4501 ataccctttt ctccaggatc tttcagagcg ttgttggcct gagaaggaac accgcctcca
     4561 gaatcgtcga agacatatcc cgtcaccgtg aagcctctga aatcgccgaa gttgacttct
     4621 aagttagaag tatcgaccat cacctcgata taattgggag tggtcgatat ataaccggac
     4681 ggatcggagc ctccggtcga tgaattgtag agatccaggg catcttccac aaccctgaac
     4741 gaaccctggg atacgttgaa gctgtaaaag cctccggaat cgctcaaggt gtatgtctgg
     4801 agcgtccact cagttccttc gagcttccac aactctattc tcacgctgtt gattccgggc
     4861 tctccggaat ctttgaacct gttgaagttc aggtcttcga agagataacc ggttatcttc
     4921 aagcctgctc caataggttt attgggaccg aattccgatg tgctgtacgc aaaagaaccc
     4981 agagaatcgc tgaaactctc ataacgcgtg atggccgtta aggcgacgat ctctagagaa
     5041 cctgccgaga ggcttccctc gaagttgccc tgcgcatcta ccgggagctc tcccagatat
     5101 gttgccccct cgccgtactc cagaccgccg tacgagtcgc cctctcccgc ggtcgcggcg
     5161 tatatctcca ccaccgctcc ctgaaagacg ggactgccgg cgttctggcc cacgtatcca
     5221 ctgacggaaa gggtattgcc gcttttcgac gccagcgata tcaccggatg atcgacacca
     5281 tcgtttccgt aatcgggatt gaggacaccg tcgttcgggt tgaccccttc gattatcacc
     5341 gctgtgagag agcgtagcag gtcgatcgcg agacctttgt tcgagtagta ggaattcatg
     5401 gagaaatcac cgaacttcga atcactgctc acccttattc cgggaccgct ggccgagccg
     5461 cgaacgatcg acttctttac cgatggaagg ttggaagaat tgaaagaaag tccgctggcc
     5521 gtcgagccag aaaccgtgag gttctcgagg gtggtcgaag tatcgccgga ggtgtagatg
     5581 ccgttgccgt ggttgactcc ggcatgtgta cctccgttgg agtctattat ggaattgctt
     5641 atcgtcaccg ggcctgcggg ggcggctaac gagatgccgt tcccatcagt tcccgcaacc
     5701 cctataccgt tgtccagaac atgtacgcct gtaacggtta aatcgcttcc ggtcaggagt
     5761 aaccccgagc cgttgtgggc gatgtaggag ttggacaggg tgccgctaga gtttattata
     5821 aggccgtaac gtctgttgag aagcacgtcc gtcgggcgtg tgccgttcgc cctcgcaccg
     5881 gtcactatac tctctagaag aaagccagga ccgttgagta tgaccggtgc gaaggaatcc
     5941 ccgaggtttc ccccgccgtt gaagaaggcc atgttcttca aaaggaaatc tattccaccg
     6001 gtggcagttc tcacgaaatg gctgaggtcg ttgcagttta tctcgatctc tttaccctga
     6061 atttgagcta ccggaacgtt ttcaactccg gccgttccgc cgccgaagac agcctgattg
     6121 ctgttgacga ctgcgccct
//
LOCUS       DAOOLY010000146         6122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15325_length_6122_cov_4.507170, whole genome shotgun sequence.
ACCESSION   DAOOLY010000146 DAOOLY010000000
VERSION     DAOOLY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15325_length_6122_cov_4.507170"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PLD15_07920"
     CDS             <1..1478
                     /locus_tag="PLD15_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB63872.1"
                     /translation="LNKLTSHMYREGKFLLAVETARAELPRFEEHKCLELANITHNMI
                     KYCCLVGDYDSSIDYGLKALELFRQFGTADDINDVIANTGGVYISMNQYEKGLEYTQR
                     ALEYAKSKNDREALSFFLNNCGIALNKLGRHDEAVVNYEEAISIKLELGKKRELCNSY
                     MNLAQVFLDIGNYSKAFEVLEKVGPIAEEISDLQILKELSFHMADYYRGNGLFDSALE
                     LLSSYVEFHTEAGSLSKIPNALKEMADIHETIGETKAALDEYKRLVSINERLYREASN
                     ARMVELESSFRVQQKMQEIEMLNSQNHELEEAKLTIERRNRELLETQEKLEIANEMLK
                     TQAETDPLTGLLNQKRMYPIIEMEIERALRYGGFLSMIMIDLDDFKRINDIYGHLTGD
                     NVLKAAAVIVKNSVRRSDYAFRYGGEEFLLLLPSTNIENASATANRLREEIPASLKPR
                     VTLSAGISTWKGEDATDFIRKTDALLYLAKENGKDRIEKEV"
     gene            1508..2788
                     /locus_tag="PLD15_07925"
     CDS             1508..2788
                     /locus_tag="PLD15_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPB63873.1"
                     /translation="MVKFFADWLFSLGMENLNIVFANIITGTIIVALAFLSKFLFEKV
                     VIKPIEHFIKKSPNKWDDILVRHHVLGRISLMIPAIVIVSFAPVFPMVEELIKRLSTA
                     YLIFIAAVVMDAILEAITDAYQFLEISRDKPIKSYITVVKIMMYIIISVIVISILTNT
                     SPWGILSGIGALTAILLVIFRDSLLGLVASVQISKNNLVSIGDWIEVPKYQADGDVID
                     ITLTTIRIQNWDKTITTIPSYALISESFKNWKGMFQAGGRRIKRSVFIDTSSVRFIDD
                     ELFERLHRIQILQPYLDSRKREIEEFNRKNNIDVTESVNGRRMTNIGTFRAYLNAYLR
                     SHPGTNKDLIIMVRQLQPTDAGLPLEVYVFSKDTSWVNYESLQSDIFDHIFAAVPHFG
                     LRVFQNPTGNDMRSLGNIFDVKSIDREQSDRELS"
     gene            complement(2797..4212)
                     /locus_tag="PLD15_07930"
     CDS             complement(2797..4212)
                     /locus_tag="PLD15_07930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB63874.1"
                     /translation="MNRFRSFGVILVVALIIAAGFVAAFIINFNSYRAIIKQDIYNIT
                     RLVSTNVFSEIQNLLVKPVYVSLTMANDSFVKKWLEEEHEEDRTRISNYLSGIKDKYS
                     YSSAFLISASTLNYYNVEGLFKTVSRKDPHDDWYYSFVDENSEFRLDIDTDQANRNLL
                     SLFVNCRIDDSEGNLLGVTGVGLEISGIREIFSNYNRLLDIDTYLIDSHGIVRLHEDE
                     DMILKGSIFDDPELALYKARILENLEDFDVIEYKNTPFENYLVSRYIAETGWYLIVTK
                     DTTPLNNSFRKQLLYDIVIITIVLALFTIIALKVISVNQKRLSTIAMTDALTGIYNRR
                     ALMEILPIELSRGKRLLYPITFMMIDIDGFKKINDLHGHIEGDRVLREFGGILQISIR
                     KQFDSAFRYGGDEFLVCLPDCGREDVNEVIERIRKLASIKEIAFSTGVHTVDPKSDNF
                     DLLDILSLIDKEMYVSKGAKN"
     gene            4628..5281
                     /locus_tag="PLD15_07935"
     CDS             4628..5281
                     /locus_tag="PLD15_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HPB63875.1"
                     /translation="MKTITRKELTEIIRPGSSAMIGGFLGCGTPDEIIDELIVSRIGG
                     LTVIANDTAFPDRGIGRLVVNRLVSRAIVSHIGTNPETQRQMIEGEIEVELVPQGTLA
                     ERVRARGVGLGGILTPTGVGTVVEEGKETLVVGEQKYLLELPISAEFAFIKAKRADYC
                     GNLSYSLTARNFNPLMAMAAECVIAEVEEVVPVGSISPEEIHTPGVIVDYILVRGCR"
     gene            5281..5931
                     /locus_tag="PLD15_07940"
     CDS             5281..5931
                     /locus_tag="PLD15_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPB63876.1"
                     /translation="MDPREVIARRVAKELKDGDLVNLGIGIPTLVANYIPSGVNVFFQ
                     SENGIIGMGPEPLEGMENPDMTNAGGMFVTALPGAMAFDSAMSFGFIRGGHLDITVLG
                     ALQVDEKGMLANWMIPGKMIPGMGGAMDLVTGAKKVIVAMTHTGKGTPKIVRKCNFPL
                     TSIRPVDLIVTELAVIEPGPEGLVLKEVAPNVSIEEIQKSTDARLIISDRLCEMTL"
BASE COUNT         1745 a         1392 c         1526 g         1459 t
ORIGIN      
        1 ttctcaacaa acttacgagc catatgtaca gagagggcaa gtttttgctc gcggttgaaa
       61 cggcacgcgc tgaacttcca agatttgaag aacataaatg tctcgaactg gccaacatca
      121 cccacaacat gatcaagtat tgctgcctcg tcggcgatta cgatagttcg atcgattacg
      181 gactgaaggc tcttgagctt ttccgccagt tcggtacggc cgacgatatt aacgatgtga
      241 tagccaacac aggtggagtc tatatatcca tgaaccagta cgaaaagggt ctggagtaca
      301 cacagagagc cctcgaatac gcaaagtcca aaaacgacag ggaggcgctt tccttctttc
      361 ttaacaactg tggaatcgct cttaacaaac ttggacgcca cgacgaagcc gtcgtaaatt
      421 acgaagaggc catatcgatc aaactggaac tgggtaaaaa aagagagctc tgcaacagtt
      481 acatgaacct tgcgcaggtc tttctggaca tcggaaacta cagcaaggca ttcgaagttc
      541 tcgagaaggt agggccgatc gccgaggaga tttccgatct acagattctg aaagagttga
      601 gtttccacat ggccgattac tacagaggaa acggtctttt cgacagcgcc ctggagctcc
      661 tgtcgtcgta tgttgaattt catacagagg cggggagtct ctccaagata ccgaacgcat
      721 tgaaggaaat ggcggacatt cacgagacga tcggcgaaac taaagccgcg ctcgatgagt
      781 acaagagact ggtttcaata aacgaaaggc tttacaggga ggccagtaac gcaagaatgg
      841 tcgagctcga atcgtccttc agagtacaac agaaaatgca ggagatcgaa atgctgaaca
      901 gccagaacca tgaactggaa gaagcgaaac tcacaataga gagacgcaac agggaactgc
      961 tggaaaccca ggagaagctc gaaatcgcca acgagatgct gaagacccag gccgaaaccg
     1021 atcctcttac cggtcttcta aaccagaaga gaatgtaccc tatcatagaa atggaaattg
     1081 aaagagcgct cagatacggg ggattcctgt ctatgatcat gatagatctg gatgatttca
     1141 agagaataaa cgatatttac ggtcatctca ccggagacaa cgtgctgaaa gccgcggcgg
     1201 tgatcgtaaa aaactctgta aggcgcagcg attacgcttt caggtacggc ggcgaagagt
     1261 tcctgttgct actgccttcg acgaatatcg aaaacgcctc cgcaacggcc aacagattga
     1321 gggaagagat accagcctct ctgaaaccta gagttacgct gagcgcaggc attagcacct
     1381 ggaagggtga agatgccaca gacttcatca gaaagaccga cgcgctgctg tatctggcaa
     1441 aagaaaatgg aaaggaccgc attgaaaaag aagtctaacg tatcgtagca gcattggagg
     1501 cgatgaagtg gtcaaattct ttgccgactg gcttttttca ctgggaatgg agaatttgaa
     1561 tatcgtgttt gccaacataa tcaccggaac gattatcgtg gctctggcct ttctttcgaa
     1621 attccttttc gaaaaagtcg tgataaaacc gatcgagcat ttcataaaga agtcacccaa
     1681 caaatgggat gacatacttg tcagacacca cgttctggga aggatctccc tgatgatccc
     1741 ggcgatagtg atagtctctt tcgcgcccgt cttccccatg gtggaggagc ttataaaaag
     1801 actatcgacg gcctatctta tattcatagc cgccgttgtc atggatgcga tactcgaagc
     1861 tataactgac gcttaccagt tcctggaaat ctccagagac aagcctatca agagttacat
     1921 aacggtagtc aagataatga tgtacataat aatcagcgtc attgtcatat ccatacttac
     1981 aaacacttct ccctggggta ttctcagcgg tatcggcgcc ctgacggcga tactcctggt
     2041 gattttcaga gactcgcttc tgggccttgt ggcaagcgta cagatatcca aaaacaacct
     2101 ggttagcatc ggtgactgga tcgaagtccc caagtaccag gccgatggcg acgttataga
     2161 tataaccctg acgactatca ggattcaaaa ttgggacaag actatcacga cgatcccctc
     2221 ttacgcattg atttccgagt ctttcaagaa ctggaaggga atgttccagg ccggtggcag
     2281 gaggataaag cggtcggttt tcatagatac ctcgagcgtc cgctttatcg acgacgagct
     2341 cttcgaaaga ctccacagaa tacagattct gcaaccttat ctcgacagca ggaaaaggga
     2401 aatagaggag ttcaacagga aaaacaacat cgatgtgacc gaatcggtca acggcagaag
     2461 aatgaccaac ataggaacgt tcagggccta cctgaatgca tatttgagaa gtcacccggg
     2521 aacaaacaaa gatctcataa tcatggtcag acagcttcag cctaccgacg ccggacttcc
     2581 actggaggtt tatgtcttca gcaaagatac atcctgggtt aactacgagt cgttacagtc
     2641 ggatatcttc gaccacatat tcgccgccgt tcctcacttc ggcttaaggg tcttccagaa
     2701 ccccaccggc aacgatatgc gttctcttgg caatatcttt gacgtaaaga gtatcgatcg
     2761 tgagcaatcg gacagagaac tttcctaggc cggagatcag ttcttcgcgc ctttgcttac
     2821 gtacatttcc ttgtcgatca gcgagagtat atccagtaaa tcgaaattgt cgcttttcgg
     2881 atccacggtg tgcactcctg tagagaaggc aatttccttt attgatgcga gtttcctgat
     2941 tctttcgata acctcgttaa catcttctct gccacaatcg ggaagacaga cgaggaactc
     3001 gtcgccgccg tatctgaagg ccgagtcgaa ctgcttgcga atactaatct gaagaatgcc
     3061 gccgaactcc cttagcactc tgtcgccttc gatatgaccg tgaaggtcgt ttatcttctt
     3121 gaatccgtcg atatctatca tcatgaaagt tatgggatac agaagcctct ttccacgcga
     3181 cagctcgatt ggaaggattt ccattagcgc ccgcctgttg tatatacccg tcagggcatc
     3241 ggtcatagcg atcgtgctta acctcttctg gttaaccgaa ataaccttca aggctattat
     3301 cgtgaagagg gccagaacta tggtaattat tacgatatcg tacagcaatt gcttacggaa
     3361 agaattgttc aacggagtgg tatctttggt cacaataaga taccagccgg tttcggcgat
     3421 gtacctggat accagataat tttcgaaagg cgtattcttg tattcgatca catcgaaatc
     3481 ttcgagattt tcaagaattc tggctttgta aagcgccagc tctgggtcgt cgaagatgct
     3541 gcccttcagt atcatatctt cgtcctcgtg gagtctcacg ataccgtgcg aatctataag
     3601 gtaagtatct atatccagca acctgttgta gttggaaaag atctccctta tcccggaaat
     3661 ctccagacct acgccggtta ctccgagaag gttgccttct gaatcgtcga tcctgcagtt
     3721 gacgaaaaga gaaagcagat ttctgttggc ttgatcggta tcgatgtcca gacgaaattc
     3781 ggagttttca tccacaaagg agtaatacca gtcatcgtgt ggatctttcc tgctaactgt
     3841 cttgaataac ccttcaacgt tgtagtaatt caaagtactg gcggagatca gaaaggccga
     3901 actatatgag tacttatctt ttataccgga aagatagttg ctgatcctgg ttctatcttc
     3961 ttcatgttct tcttcaagcc acttcttaac aaaggaatcg ttggccatcg tgagagaaac
     4021 atatacgggt ttgaccagta aattctggat ctcggagaag acgttcgtcg atacgagtct
     4081 ggtgatgttg taaatatcct gcttgatgat agcacgatac gaattgaagt tgatgataaa
     4141 ggctgctacg aaccccgctg cgattattag agcgacgacc aggataactc cgaaacttcg
     4201 aaatctgttc aaattaatac tcccacccag ccagtcaccc ggataaacat cgaatccgcg
     4261 ctcagggatt aatgaaagta ttatatacat tatgaaggcg aagttctgta actaaagtta
     4321 cattgtgagt ttctattctc tcttccagta gttctcgtgc tcccggtctt tgaaacttcc
     4381 cttgcaagca gacaggccgc cagaggcgac tgaaaaatac gcggcgatac tggatttagt
     4441 atcgaaatac ttagatttgc acagaagcgg cacaaaaagc atcggcgtcc gaaaaagcta
     4501 taattgcttc agtttgcttc agccggcgat ataagagatt cgaaagcgat cccatctata
     4561 ggttatactc tctgcaaggc cacatagtac ttatcgatac gcgccgttaa cgagaggagg
     4621 atacagtttg aaaacaatta ccagaaagga actcacggaa ataatcagac caggttcgtc
     4681 agcgatgatt ggcggcttcc tcggctgcgg cacgcccgat gagataatcg acgagctgat
     4741 tgtctcgaga atcggtgggc tgacggtgat agcaaacgac acggctttcc ccgacagagg
     4801 aataggcagg ctcgtcgtga acaggctggt gagcagggcg attgtctctc acataggaac
     4861 caaccccgaa acccagcgtc agatgataga aggagagatc gaagttgaac tcgttccaca
     4921 ggggacactt gccgaaaggg tgcgtgccag aggagtgggg ctcggcggca tacttacccc
     4981 taccggtgtc ggaacggttg tagaagaagg gaaagagacc ctagtggtag gagagcagaa
     5041 atacctgctg gaactcccga taagcgctga atttgccttt ataaaggcga agagagccga
     5101 ttactgtgga aatctctcat actccctcac ggccagaaat ttcaatcccc taatggcaat
     5161 ggccgccgaa tgtgtcatag ccgaagtgga ggaggtcgtg cccgttggtt cgataagtcc
     5221 agaggaaata cacactcccg gcgtaattgt ggattacata cttgtaaggg ggtgtcgctg
     5281 atggatccca gagaggtaat agccagacgc gttgctaaag aacttaaaga cggagacctg
     5341 gtgaatctgg gaataggaat tccgacactg gtggccaatt atatcccatc gggcgtgaat
     5401 gttttcttcc agtcggaaaa tgggataatc ggaatgggac cggagccgct tgaaggcatg
     5461 gagaacccgg atatgaccaa cgcgggcggg atgttcgtga cggcccttcc cggtgctatg
     5521 gccttcgata gcgcaatgtc cttcggtttc ataaggggag gccatctcga tattacggtg
     5581 ctcggcgcct tacaggtgga tgaaaagggt atgctggcca actggatgat tcccggaaag
     5641 atgattcccg gcatgggggg cgctatggac ctggtaaccg gcgcgaaaaa agtgatagta
     5701 gcgatgactc atacgggtaa aggtaccccc aaaatagtta gaaaatgtaa ttttccgttg
     5761 acttcaatca gacctgtaga tctcattgtg acggaacttg ccgtaataga gcccggtccg
     5821 gaggggctgg ttttgaagga agtggccccg aacgtatcga tagaggagat acaaaaatcg
     5881 acggatgcgc ggcttataat atccgaccgg ttgtgtgaaa tgacactgta gtattaaaat
     5941 cattgggagc ttgtatatgc aaaaaactaa cgcgactaga attctcgata aaatgggaat
     6001 agagtacgag gttctcgaat acgatgtgga tgaagatcaa ctgggtgccg agcacgtggc
     6061 tgaagaattg gggataagcg ccgacatgac catgaagacg ctggttgtaa agggtgacag
     6121 aa
//
LOCUS       DAOOLY010000147         6082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15468_length_6082_cov_4.088269, whole genome shotgun sequence.
ACCESSION   DAOOLY010000147 DAOOLY010000000
VERSION     DAOOLY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15468_length_6082_cov_4.088269"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PLD15_07945"
     CDS             complement(<1..1608)
                     /locus_tag="PLD15_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB63877.1"
                     /translation="MPVSTYKRKVNTLQINIASPERIKGWSSGEVKKPETINYRTFKP
                     ERDGLFCEKIFGPTKDYECTCGKYKGKKYEGTVCERCGVRVESKEARRRRMGHIELAA
                     PVTHVWYLKSAPSVLSSLLNIPAKDLENIIYYGSKRVIERLYVVTDPKDTDFFSGMTM
                     YQTEYEIYTKKLDFEVEQGYKVKSPQGPVTTEIDGKVTIERIDSNTQREMNWIIIRDE
                     TGIEKKYPTFEGSLVYVQNGDEVHKGDTLADRFLFEDEVLSSTEYRVFDEYYPGKFEV
                     ETDTESDRPIVIITEIDPSVAEETGRAVGEVLIENEYEAYRELYPGKITCDYGAAAIK
                     KLLQGMDLEELQVAIEHELKSIPKSSARALKLLRRLKYIKDFIASKNRPDWMVMDVVP
                     VIPPDLRPMIQIEGGRFATTDLNDLYRRVINRNNRLAKLLQIGAPDIIIRNEKRMLQE
                     AVDSLIYNGRVGKAVSDKSGRPLKSLTDLVKGKKGRFRRNLLGKRVDYSGRAVIVVGP
                     DLKIHECGIPKRVAMELFKPFVLSKLLH"
     gene            complement(1649..5173)
                     /locus_tag="PLD15_07950"
     CDS             complement(1649..5173)
                     /locus_tag="PLD15_07950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB63878.1"
                     /translation="MRKANFGKRERWVFGRVYEPLEIPNLIEIQTRSFNRFIDEGLLS
                     VLKKYSPIKSQIQRSDLKKGEKGFSLDFVETRIGEAKHSIQECKDKGLTYSVPLYVTV
                     RITDLYTGEIKEEEAFFGYLPYMTDNASFIINGAERVIVNQLVRSPGVYFVDEPTKAA
                     SGSLPIYVAHFLPVKGAWFEILLNPNKQIMQARIDRRRKLNFFVFLKTLGYEDDVELL
                     KLFENVIDATDEDELLANVGSMVLKEIRTPGGEVIAEKGKPLTEEMVAKIVESDVKTV
                     SLPLPIAMRTYLALQKTYGQGMSEDDAFIELFRKLRPGEIPRINAAKSYINNLYFSPD
                     RFDFSDVGRYKVNARLKKVYQDYLREVEGKKVSKDTDYAESSGMLTPLDVVLAARHLT
                     EIESRPELLDTKDHLGNKRVRTVGELMETEFEKAFIKIHKLLEEKLTIYTSFDKITVQ
                     SLVNVRTLMTTLHQFFATSQLSQFMDQVNPLAELTNKRRLSAIGPGGLKREHARFEVR
                     DVHHSHYGRMCPIETPEGANIGLMTSMATYATIDKYGFLLTPYRKVEKGKVTDDIVYL
                     AADEEERYNIAHSTVKMDGNGKIADDSVEIRYAGEVRYVKKEDVDYMSVSPKQIVSIS
                     TSLIPFLEHDDANRALMGSNMQRQAVPVVKSEAPIVGTGVEGVAARDSGYVVLAKHGG
                     RVNKVDGRKISIQRLDEKSEPVLDINGKPIEDEYLLHKFVRTNQDTAVNQRPIVSVGE
                     IVREGDAIADGPSTDMGELALGKNVLVAFMPWEGYNFEDAILVNQELLGEDTFTTIHV
                     EMYETIARDTQLGPEEITADIPNVSKESLKNLDESGIVRVGAYVTSQDILVGKVTPKG
                     ESETTPEEKIIRSVFGDRGRDVKDSSLKLPHGVEGRVIDVKVFDKEEVSELGSGINKL
                     VKVFVGIRKPLDVGDKLAGRHGNKGVVSNIIPKEDMPFLPDGTPIHLVLSPLGVPSRM
                     NVGQVLETHLGWLGKLTNRYFATPIFDGATEEEIMTELYHVRRELGLDEGDSEGQKSG
                     KVTLRDGRTGEPFDYPVVVGVMYMLKLVHIAKDKIHARSTGPYSLIHQQPLGGKAHFG
                     GQRFGEMEVWALEAHGAAHTLNEMLTIKSDDIKGRNDVYKAILKGINIPEPGIPESFK
                     VLIKELQGLSLDVKVYDQNSIELDVDKL"
     gene            complement(5280..5666)
                     /gene="rplL"
                     /locus_tag="PLD15_07955"
     CDS             complement(5280..5666)
                     /gene="rplL"
                     /locus_tag="PLD15_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPB63879.1"
                     /translation="MTKEELISAIKAMTVAELAELVKALEEEFGVSAAAPVAVAAAPV
                     AGGAAVEEEEEQTEFKVVLKSFGAKKIDVIKVVRAITGLGLKEAKDLVEKAGTPEGVV
                     KENMPKAEAEELKKQLEEAGAEVELK"
     gene            complement(5688..>6082)
                     /gene="rplJ"
                     /locus_tag="PLD15_07960"
     CDS             complement(5688..>6082)
                     /gene="rplJ"
                     /locus_tag="PLD15_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPB63880.1"
                     /translation="RARFSVTKNTLVRLALANAGYEESEWMNTVSSTTAVLTIDDEDP
                     VSALRIMIDFNKSNKILPIIKGGYLERGFFRGDQAIELSKLPSRNQLIAMVVGGFAAP
                     ITGLVYTLNGVLTKFLYALNAIKDKKAE"
BASE COUNT         1236 a         1677 c         1404 g         1765 t
ORIGIN      
        1 atgaagtagc ttggagagaa cgaagggttt gaacaactcc atcgctactc tcttggggat
       61 tccacactcg tgtatcttca gatccggtcc tacaacgata acggctctac cggaataatc
      121 gactcgctta ccgaggagat tccttctgaa ccttcccttc tttcctttga ccagatccgt
      181 cagagatttc aaaggccttc ccgatttgtc ggatacggcc tttccaaccc ttccgttgta
      241 gatgagcgaa tcgacggcct cctggagcat cctcttttca tttctgatta ttatgtcggg
      301 ggctccgatc tgtaagagtt tggcaagccg gttgtttctg tttataactc tcctgtacag
      361 gtcgttgaga tcggtagtgg cgaaccttcc gccttcgatc tggatcatgg gtcgtagatc
      421 cggtggaatg accggcacaa cgtccatcac catccagtcg ggcctgttct tggaagctat
      481 gaaatccttg atgtatttga gccttctgag aagcttaaga gccctcgcgc tgctcttggg
      541 tatagacttc aactcgtgtt ctatggccac ctgcaactcc tcgagatcca ttccctgaag
      601 cagcttcttt atcgcggcag ctccgtaatc gcaggtgatt ttccccggat agagttccct
      661 gtaggcttcg tactcgtttt cgatgagcac ctcgccaacc gctcgaccgg tctcctcggc
      721 aacggacgga tcgatctcgg ttattatcac tatcggcctg tcgctttcgg tatccgtttc
      781 cacttcgaac ttgccgggat agtattcatc gaagactcta tactccgtag aagagaggac
      841 ctcgtcttcg aagaggaagc gatcggccag agtgtctccc ttgtggactt catctccgtt
      901 ctgtacgtag accagagagc cttcgaaggt ggggtacttc ttctctatac cggtttcatc
      961 cctgatgatt atccagttca tctctctctg cgtgttgctg tcgattcttt cgatcgttac
     1021 cttaccatct atctctgtgg taaccggacc ctgcggagac ttgactttgt aaccctgttc
     1081 cacttcgaaa tccagtttct tggtgtatat ctcgtattcg gtctgataca tcgtcatacc
     1141 cgagaagaag tcggtgtctt tcgggtcggt aacgacgtaa agcctttcga tgactctttt
     1201 ggagccatag tagattatgt tttccagatc tttggccggt atgttcagaa gagaagagag
     1261 aacgctcggc gcgctcttca agtaccagac gtgtgtcacc ggtgcggcca gttcgatgtg
     1321 acccattctt cttcttctgg cctctttgga ttcgaccctc actccacacc tttcacagac
     1381 ggtgccttcg tacttcttgc ccttgtactt tccgcaagta cattcgtagt ctttggtcgg
     1441 tccgaaaatc ttctcgcaga agagtccatc tctttcgggc ttgaaagtgc ggtaattgat
     1501 cgtctccggt ttctttacct caccgctcga ccagcctttg attctctccg gcgaggcgat
     1561 gttgatctgg agagtgttga cttttctttt atacgtgctc acaggcatct gcttccctcc
     1621 ttgaggggta gctcccttct atttatcttc atagtttatc cacatccagt tcgatgctgt
     1681 tctggtcgta gaccttaaca tccagagaga ggccctggag ctcctttatc aacactttga
     1741 aactctcggg aattccaggt tccggtatgt taataccctt cagtatagcc ttgtacacat
     1801 cgtttctacc ctttatatcg tcggacttta tggtcagcat ttcgttgagt gtgtgggccg
     1861 ctccgtgtgc ctccagcgcc cagacctcca tctctccaaa tctctggcca ccgaaatgtg
     1921 ccttacctcc cagaggttgc tggtgaatca gggagtaagg cccggtagaa cgggcgtgga
     1981 ttttatcttt ggctatgtgc accagcttga gcatgtacat gactccaacg actaccgggt
     2041 agtcgaacgg ttcgccggtc ctgccgtccc tcagagttac tttaccagat ttctgtcctt
     2101 cgctgtcccc ttcgtccaga cctagctctc ttctcacatg gtaaagctcc gtcattattt
     2161 cttcttcggt tgcaccatcg aatatcggag tcgcgaagta tcggttggta agttttccaa
     2221 gccagcccag atgggtctca agaacctgtc ccacgttcat tctcgatgga acgccgagcg
     2281 gagaaagcac taaatgaatt ggtgtaccgt ctggaaggaa gggcatatct tcctttggaa
     2341 ttatattaga aacgacacct ttgtttccgt gacgaccggc cagtttgtct ccgacatcga
     2401 gcggctttct gatccccacg aagactttga cgagtttgtt gatacccgaa cctagttccg
     2461 agacttcctc cttgtcgaag accttcacgt cgatcaccct gccttcgaca ccgtgcggga
     2521 gtttcagtga agagtccttg acatccctac ccctgtctcc gaagacggac ctgattatct
     2581 tctcctcagg cgtagtctcg gactctccct tgggtgttac cttaccgacg aggatatcct
     2641 gcgacgtcac gtaagctccc accctcacga ttccgctttc gtcaagattt ttgagcgact
     2701 ccttcgaaac gttcgggata tcggcagtga tctcttcggg tcccagctgg gtatctctgg
     2761 cgatcgtttc gtacatctcc acgtgtatcg tggtgaatgt atcctcaccg aggagctcct
     2821 ggttcaccag tatggcgtcc tcgaagttgt aaccctccca gggcatgaag gccaccagaa
     2881 cgttctttcc aagggccagc tcgcccatat ccgtcgaagg gccgtcggct atggcgtcgc
     2941 cttctctaac gatctccccg accgatacta tgggtctctg attaacggcc gtgtcctggt
     3001 tcgttctgac gaacttgtgg agaaggtact catcttctat cggttttccg ttgatatcca
     3061 gaaccggttc acttttttca tccagtctct gaatggatat ttttcttcca tcgaccttgt
     3121 tcacacggcc gccgtgcttt gccagaacca cgtagccgga atctctggcg gcgacacctt
     3181 cgacacccgt accgactata ggcgcctcgg acttgactac cggaacggcc tgtctctgca
     3241 tgttagaacc cattagagcc ctgttggcat catcgtgctc gaggaacggt atcagggagg
     3301 tcgaaatcga tacgatttgt ttgggggaaa ccgacatata atcgacatcc tccttcttga
     3361 cgtacctgac ctcaccggcg taccttatct ctacactgtc gtcggcaatt ttgccgtttc
     3421 cgtccatctt caccgtcgag tgcgctatgt tgtacctctc ctcttcgtcg gccgccagat
     3481 acactatgtc gtcggtgacc tttccctttt caaccttcct gtaaggtgtg aggaggaatc
     3541 catacttatc tatcgtcgcg tacgtggcca tcgatgtcat aagacctatg ttcgcacctt
     3601 cgggagtctc tatcggacac atcctcccgt aatgagagtg atgcacgtct ctgacttcga
     3661 acctggcgtg ctccctcttc aatccaccgg gaccgatggc cgaaagtctt ctcttgttcg
     3721 tcagctcggc aagtggattg acctggtcca tgaactgcga cagctggctg gtggcgaaga
     3781 actggtggag cgtggtcatc agggttctca cattcaccag actctgaacg gttatcttgt
     3841 cgaaggaagt atatatggtc aacttctcct cgaggagttt gtggatcttt ataaaggctt
     3901 tttcgaactc cgtttccata agctctccga cggttctgac cctcttgttc ccgagatggt
     3961 ccttggtgtc aagcagttcg ggacggctct ctatctcggt caggtgtcgg gctgccagaa
     4021 ctacgtccag cggtgtcagc atgcccgatg attccgcata atcggtgtct ttgctcacct
     4081 ttttaccttc cacttcccta aggtaatcct ggtaaacctt cttcagtctg gcgtttacct
     4141 tgtatctgcc gacatcggaa aaatcgaatc tgtcgggact gaaatagagg ttgtttatgt
     4201 agctcttggc ggcgtttatc ctgggaattt ctcccggtct gagttttctg aagagctcta
     4261 tgaaggcgtc gtcctcgctc ataccctgcc cgtaggtctt ttgcagcgcg agataagttc
     4321 tcatggctat gggtagagga aggctgacgg tcttgacatc ggattcgacg attttcgcta
     4381 ccatctcttc ggtcagcggc tttcccttct cggctatcac ttctccgccg ggggttctga
     4441 tctccttcag aaccatactg ccgacattgg ccaggagttc gtcctcgtcg gtagcgtcta
     4501 tcacgttttc gaaaagcttc aacagctcaa cgtcatcttc atagcccaga gtcttcaaga
     4561 agacgaagaa attcagcttt cttcttctgt cgattctggc ctgcattatc tgcttgtttg
     4621 gatttaaaag aatttcgaac caggcgccct tcaccggaag aaagtgggca acatatatgg
     4681 ggagggaacc cgaggctgct ttcgtaggtt catcgacgaa gtaaacgccg ggagatctca
     4741 ccagctgatt gacgatgact ctttccgcgc cattgattat gaaggaagcg ttatcggtca
     4801 tgtagggaag gtacccgaag aaagcttcct cttctttgat ctctccggtg taaagatcgg
     4861 tgattctcac agttacgtac agggggacgg agtaagtgag acccttatcc ttgcactcct
     4921 gtatggaatg cttcgcctca cctatacggg tttcaacgaa atccagtgag aagccttttt
     4981 cacccttctt gagatcggat ctctggatct gcgacttgat gggtgaatac ttcttcaaaa
     5041 cactcaaaag cccttcatcg atgaacctgt tgaaggaacg agtctggatc tcaatgagat
     5101 tcgggatttc aaggggttca taaactcttc caaagaccca tcgctctctt ttgccaaaat
     5161 tggcctttct catcagatca cctctcgtac atttaagcac accaaaccca catcagagag
     5221 tctctctgat gtgggtgtag aaaattatat cgaaactatg taaccaatca attgcctaat
     5281 tacttcagtt cgacttcggc gccggcttct tcaagttgct tcttgagctc ttcagcctct
     5341 gccttcggca tattctcctt gactacacct tcaggtgtac cggccttctc gactagatcc
     5401 ttggcttcct tgagacccaa cccggtgatg gcccttacaa ccttgataac atctatcttc
     5461 ttcgcaccga aggacttcag gacaaccttg aattcagtct gctcttcttc ttcctcaacg
     5521 gctgctccgc cggctaccgg ggctgccgcc accgctactg gtgccgccgc gctgacacca
     5581 aactcttctt ccagggcctt tacaagttcc gcaagctcgg ccaccgtcat tgcttttatt
     5641 gcgctaatga gttcttcctt agtcatctta ccacacctcc gtctttatta ttctgctttt
     5701 ttatctttaa ttgcgttcaa cgcgtacagg aatttcgtga gtacgccatt caaagtgtaa
     5761 accagcccgg ttatcggggc ggcaaaaccg ccgactacca tagcaatcaa ctggtttctc
     5821 gatggcagct tggagagttc gatagcctgg tctcccctga agaagcctct ctcgagatac
     5881 ccgcctttga ttatcgggag aatcttgttg ctcttgttga aatcgatcat tatcctcaag
     5941 gccgaaacgg gatcttcgtc gtcgatcgtg agaacggccg tcgtactcga aacggtattc
     6001 atccattccg attcctcata gccggcgttg gcgagggcaa gcctgactag cgtgttcttc
     6061 gttacgctga acctggccct gt
//
LOCUS       DAOOLY010000148         5956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_15933_length_5956_cov_5.231825, whole genome shotgun sequence.
ACCESSION   DAOOLY010000148 DAOOLY010000000
VERSION     DAOOLY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15933_length_5956_cov_5.231825"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /gene="thrC"
                     /locus_tag="PLD15_07965"
     CDS             <1..1168
                     /gene="thrC"
                     /locus_tag="PLD15_07965"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB63881.1"
                     /translation="GERKGTLEVIYDFDIVRREYSLNQSDIGKDGIWAFDFLLPFEGD
                     HFRPNLLVGNTPLYRCENLSEKYAVEVFIKDDGRNPTASFKDRASSVAAAKAVEKGYG
                     TVFCASTGNAASSLSGMAAVSKLKSVIFVPATAPQAKLTQLQVYGSKVLAIEDSYDRA
                     FDLSMEIGLSKGWYCRNSAINPYLLEGKKTCALELAFSMIDRLPDYIFVSAGDGTVLS
                     SFHKGFEDLREMGVIDRLPVIVGVQASGAAAIKKTYEGGLPFEPVDIQASSVADSICV
                     GKPRDVVKACRYTHKNKGMLIDVSDKEILEGIIEMARESGVFAEPAGATALAGFKKCA
                     RQGLLSLDSRVVIVVTGNGLKDLKSPSSIMPALRKLPPDRKIIEEEIGEDRFQS"
     gene            1146..1619
                     /locus_tag="PLD15_07970"
     CDS             1146..1619
                     /locus_tag="PLD15_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB63882.1"
                     /translation="MKIDFKVNGRDYSLEVREDMRLLDFLREELKLTGTKEGCGEGEC
                     GACTVIIDGKAVNSCLVLVPEIDGREVITVEGLERDGRLHPLQESFIEKGAVQCGFCT
                     PGMLMSAKALLDGNADPSDEEIMEAIEGNLCRCTGYYKIVQAIRDAAGKLRNGKA"
     gene            1606..2181
                     /locus_tag="PLD15_07975"
     CDS             1606..2181
                     /locus_tag="PLD15_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB63883.1"
                     /translation="MAKPERVALILLAAGTSSRFKSEKLTAFFEGKRLLQWALDSFSL
                     LELEKILVIKPGFPLGLFSPDGFRVVVNYDYETGIASSIKAGLKSIDPRSEGLVLTLG
                     DMPFIERRDVERLLAEAPDGKELTAFAFQGIKGFPTFVPETLFFDLSKIEGDRGAFAL
                     VKEGIAKFRSIEGEKRHTVDIDVPEDIESPL"
     gene            2365..3174
                     /locus_tag="PLD15_07980"
     CDS             2365..3174
                     /locus_tag="PLD15_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPB63884.1"
                     /translation="MPVDERELKSMLGGEDCKIMAGGTDLLVRIRSGRLSPSRIVSLA
                     RMKGMDGILDLGEEVSIGSRVTMSELLKCELIAASCPILPMVVREIGSPQIRNRATLA
                     GNLVNASPAGDSIMALYLSNATVVISGVNGERREKVSSFIKGPGRTTLSRDEYVGAIL
                     LEKTPGYFPQFYKVGQRSAMAIAVASVGSLISGQEIRMAFGSVAPTVAVPEKACEYFE
                     KAGEKFDDGEFARLAMLAISPIDDVRASAWYRSTVVKNLVQRTLNVWRKKG"
     gene            3177..4013
                     /locus_tag="PLD15_07985"
     CDS             3177..4013
                     /locus_tag="PLD15_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPB63885.1"
                     /translation="MLSNVKEYFRPKTVEEAFEMMEADREHSIFLSGGTIVALMGSSR
                     IERIIDLKSLRLDSVSIKEDEIVVGATTTVEAFRKDPLMRKEFGDFFYDSFSLVGSWQ
                     IRNMATIGGSIAPKLGWSDVSACLLATGAGLMVYGPDGYRRIMLSDYFALPAGEKPLI
                     THIIIKKDGWVSAFEKFSKTTFDIATVNVAISVRPERERIKAARVVCGSRPQYPMRFE
                     TIENALEELEINDVMPSIVRSLVFDNFTGGSNMLASFEYRKHLASVMAQKAAEKIKEM
                     VR"
     gene            4010..4483
                     /locus_tag="PLD15_07990"
     CDS             4010..4483
                     /locus_tag="PLD15_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPB63886.1"
                     /translation="MKITLNVNGNLHEINIDPGDYLLEILRDLGYKSVRRSCHSASCG
                     TCTVLVNGKPILSCSTFAASVDGQTIETVEGLSQGEKLHPIQEAILEEGAAQCGYCIP
                     GIVLTGEALLRENPEPSDDDIKLALNGNVCRCTGYESQNRAIKKAAEAMRGDDYE"
     gene            4476..>5956
                     /locus_tag="PLD15_07995"
     CDS             4476..>5956
                     /locus_tag="PLD15_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB63887.1"
                     /translation="MSEAFETVGKPKKKLDGRALVRGKPVYTPDYDVPGALTVKLLRS
                     PHAHAIIKTIDTDQARAVEGVVAILTHEDLERIPYSRAGQGYPEPSPYDAYLLDRKVR
                     YVGDAVAIVAATSEKAALKALKLIEVEYELLDPVLNMEDSMKEGSPVIHDEEDCSGLF
                     DKSRNIAAHFEMEIGDVEGTLNGCDYVITRTYHLDTQLHAALEPHNAVTYLDPAGRLI
                     VVTSTQVPFHCRRILSRILNKPISEIRVIKPRIGGGFGGKQSIIAEPYAAIVTLKTGR
                     PARIIYTREEVSMASNIRHEMKYDITVGADGNGRIKAISMHGLSNTGAYGEHCLTTFM
                     VSGSKTLPLYNKVDAVRFGGDVVYTNLPPAGAYRGYGAPQGLFALDCAIDELAHELKI
                     DPLKVKEMNSIREGETSPIFKIMGEGREGVPQIMKSCKLQECIDLGKKAIGWDEKRGR
                     RTRRGSRVRGLGCALAMQGSGIPRVDMGAATLKMNEDGSFNLLV"
BASE COUNT         1456 a         1523 c         1818 g         1159 t
ORIGIN      
        1 cggcgagaga aagggtacgc tggaagtgat ctacgatttc gatatcgtca ggcgggagta
       61 ttcgctaaac cagtccgata tcggcaaaga cggcatctgg gccttcgatt ttctcctccc
      121 cttcgagggc gatcatttta gaccgaacct gctggtgggc aacacgccgc tttacaggtg
      181 cgagaatctg tcagagaagt acgccgtcga ggtgttcata aaggacgacg ggagaaatcc
      241 cacggcctcc ttcaaagaca gggcaagctc ggtggccgcg gccaaggccg tcgagaaggg
      301 ttacggaacg gtcttctgcg cctcgacggg caacgccgcg agttctctct cgggcatggc
      361 cgccgtttca aagctcaaga gcgtcatctt cgttccggcg acggctcctc aagccaaatt
      421 gacacagctc caggtttacg gctcgaaggt tctggcgata gaggacagtt acgacagggc
      481 tttcgatctg tcgatggaga tcggtctttc aaagggctgg tactgccgca acagcgccat
      541 aaatccctat ctgcttgaag ggaaaaagac ctgcgcgctc gaactggctt tctcgatgat
      601 cgacaggctt cccgactata ttttcgtctc ggccggcgac ggcacggttt tatcgagctt
      661 ccacaagggt ttcgaagacc tgagggagat gggagttatc gacaggcttc cggtcatagt
      721 aggagtacag gcctcaggag cggctgcgat aaagaagact tacgagggcg gcctcccctt
      781 cgaaccggtc gatattcagg ccagctcggt agccgacagc atttgcgtgg gcaagccgag
      841 agacgtggtg aaggcctgtc gttacacgca caagaacaaa ggtatgttga tagatgtttc
      901 cgataaggaa atccttgagg gtataatcga aatggccaga gaaagcggcg tcttcgccga
      961 accggccggc gcgactgccc tggccgggtt caagaagtgc gcgcggcagg gtctgctatc
     1021 gctcgacagc agggtcgtga tcgtggtaac gggtaacggt ctcaaagatc tcaagagccc
     1081 ttcgtcgatc atgccggcgc ttcgcaagtt accgcccgac aggaagatta tcgaggagga
     1141 aataggtgaa gatcgatttc aaagttaacg gtagggatta ctctctcgaa gtgagagaag
     1201 acatgaggct tctggacttc ctcagggaag agctgaagct caccggaacc aaggaaggct
     1261 gcggtgaagg tgaatgcgga gcctgcacgg tgataatcga cggcaaggcc gtcaactcct
     1321 gcctggttct cgtaccggaa atagatggaa gggaagttat aacggtggag ggcctggaaa
     1381 gagacggccg gctgcatccc cttcaggagt cgtttataga gaagggggcc gtccagtgcg
     1441 gattctgcac gcccggcatg ctgatgtcgg ccaaagcgct cctggacggg aacgcagatc
     1501 cctccgacga ggagataatg gaggccattg aaggcaacct ctgccggtgt actggctact
     1561 acaagatagt tcaggctata agggatgcgg ccggaaagtt gagaaatggc aaagcctgaa
     1621 agggttgcgt tgattcttct ggcggccggc acttcgagcc gcttcaagtc ggaaaagctg
     1681 acggctttct tcgaaggcaa gagactgttg cagtgggcgc tcgacagttt cagccttctc
     1741 gagctggaaa agatactggt gatcaaaccg ggcttcccgc tcgggctctt ttcgcccgac
     1801 gggttcaggg tagtggtgaa ttacgattac gaaacgggca tcgcctccag cataaaggcc
     1861 ggcttgaaga gcatcgatcc ccggtcggag ggactggtac tgacgctggg agatatgccc
     1921 ttcatagaac ggagggacgt agaaagactc ctcgccgaag ccccggatgg aaaggagctg
     1981 acggccttcg cttttcaggg cataaaaggc tttcccacct tcgttccgga gacgctcttt
     2041 ttcgatcttt cgaagatcga gggcgacagg ggagccttcg cgctggtcaa ggaagggatc
     2101 gcgaagttca gatccataga gggagagaaa cgccatacgg tggatataga tgttccagag
     2161 gatatagaga gtccgctttg agggaaagtg ttccaaatta caggtaaaag acgggactgg
     2221 ataaccagtt catacccgga gcccggaact tcaatacttg tagacggttc taaacgctct
     2281 gatatatatc gatgtttcgt cgggactcgc ggttcgcgat gaggtttgaa agggtggtgg
     2341 tagcgtattt ccttcaaatt gttgatgccc gttgatgaaa gagaactcaa gagcatgctc
     2401 ggcggcgaag actgcaagat aatggccggc ggtacggatt tgctggtcag gataaggtct
     2461 ggcaggctat ccccgtccag gatagtcagt ctcgccagaa tgaagggcat ggacggcata
     2521 ctcgatctgg gcgaggaggt ttcgatcggg agcagggtga cgatgagcga actgctcaag
     2581 tgcgaactca tagccgccag ctgtccgatt ctgccgatgg tcgttcgcga gatcggaagc
     2641 ccgcagataa ggaacagggc aactctggcg ggcaacctgg taaacgcctc tcccgccggg
     2701 gactcgatca tggctctcta cctctcgaac gcgacggtgg tgatctccgg cgtgaacgga
     2761 gaacggaggg agaaggtttc ctccttcata aagggcccgg gaaggaccac gctctcgaga
     2821 gacgagtacg tcggcgcgat actgctcgaa aagaccccgg gttactttcc ccagttctac
     2881 aaggtagggc agaggagcgc catggcgatc gccgtcgctt ctgtcggctc tttgatatcc
     2941 ggacaggaga tcagaatggc tttcggctcg gtcgcgccca cagtggccgt gccggagaag
     3001 gcctgcgagt acttcgagaa ggccggggag aaattcgacg acggggagtt cgccagactg
     3061 gctatgcttg cgatatcgcc gatagacgac gtgagggcca gcgcctggta cagatcgacg
     3121 gtggtcaaaa accttgttca aagaactcta aatgtctgga gaaagaaggg gtaatcatgc
     3181 tttcgaatgt aaaagagtac ttcaggccaa agacggtcga ggaagccttc gagatgatgg
     3241 aggccgacag ggagcattcg atcttcctct cgggcgggac gatcgtcgcg ttgatgggat
     3301 cctcgagaat agaacggata atagatctga aaagtctcag gctcgattcg gtctccatca
     3361 aggaggacga gatagttgtc ggcgcgacca ccacggtcga agccttcagg aaggacccgc
     3421 tgatgaggaa ggagttcggc gatttctttt acgacagttt ctctctggtc ggctcctggc
     3481 agataaggaa catggccacg attggcggat cgatcgcccc caagctcggc tggtctgatg
     3541 tgagcgcctg cctgcttgcg accggagccg gtctaatggt ctatggcccc gatggttata
     3601 gaagaataat gctctcggat tacttcgctc ttccggccgg tgaaaagcct ctaataacac
     3661 atataatcat aaagaaggat ggctgggtat cggccttcga gaagttttcc aaaactactt
     3721 tcgatatcgc gaccgtgaac gtcgccatat ccgtcaggcc ggagagagaa cgtataaagg
     3781 ccgccagagt cgtatgcggt tcgagaccgc aatacccgat gaggttcgag acgatagaga
     3841 acgccctgga agaactggag atcaacgacg taatgccctc tatcgtacgt agtctggtct
     3901 tcgacaactt caccggtggc tcgaacatgc tcgcctcctt cgaatacaga aagcacctcg
     3961 cttcggtgat ggcacagaag gccgccgaga agataaagga gatggtcaga tgaaaataac
     4021 tctcaacgtc aacggaaacc tccacgagat caacatcgat ccgggcgatt atctcctcga
     4081 aatactgcga gacctgggct acaagagcgt gagaagaagc tgccactcgg ccagttgcgg
     4141 cacctgtacg gtgctggtga acggaaaacc gatcctttcc tgttccacct tcgccgcctc
     4201 cgtggacggc cagacgatcg agaccgtcga aggactttcg cagggcgaga agctccatcc
     4261 aattcaggaa gcgattctgg aggagggagc ggcgcagtgc ggctactgca taccgggaat
     4321 cgttctgacg ggcgaggcgc tccttaggga gaatcccgaa ccttcagacg acgatataaa
     4381 gctggccctg aacggcaacg tctgtcggtg caccggctac gagtcccaga acagggcgat
     4441 aaagaaggcg gccgaagcga tgagaggtga cgactatgag tgaagccttt gaaaccgttg
     4501 gaaagccgaa gaagaaactc gacggcagag cgctggtgag agggaaaccc gtatatactc
     4561 cagattatga tgtccccggt gcgctcaccg tgaagcttct gcgctctcca cacgctcacg
     4621 cgataataaa aaccatagat accgatcagg cccgcgccgt cgagggcgtc gtcgcgatcc
     4681 tgacgcacga ggatctggaa aggattccct actccagagc cggccaggga taccccgagc
     4741 cttctccata cgacgcttac ctgctggaca ggaaggtgcg ctacgtggga gacgcggtcg
     4801 cgatcgtggc ggctacctcc gagaaggcgg ccctgaaagc cctgaaattg atagaggtcg
     4861 agtacgaact tctcgatccc gttctgaata tggaagactc gatgaaggaa ggatcacccg
     4921 tgatacacga cgaggaagac tgctcgggcc tcttcgataa atcgagaaac atcgcggcgc
     4981 acttcgaaat ggagatagga gacgtcgagg gaacgctcaa cggttgcgat tacgtcatca
     5041 cgagaaccta ccacctcgac acacaattac acgccgcgct tgagccgcac aacgcggtga
     5101 cctacctcga tccggccggc cggctcatag ttgtgacctc tacgcaggtc ccctttcact
     5161 gccggcgcat cctgtcgaga atactgaaca aaccaatatc ggaaatacgc gtcataaagc
     5221 ccagaatagg cgggggcttc ggcgggaagc agtcgataat agccgagcct tacgccgcga
     5281 tcgtgacgct caagaccggg aggccggcca gaataatcta caccagggaa gaggtctcga
     5341 tggcctcgaa catacgacac gagatgaagt acgacataac ggtcggggcc gacggcaacg
     5401 gcaggataaa ggccatctcc atgcacggtc tctccaacac gggtgcctac ggcgagcact
     5461 gtctgacgac cttcatggtc tcgggctcga aaactcttcc gctgtacaac aaggtcgatg
     5521 ccgtccgctt cggcggggac gtggtttaca ccaacctgcc gcctgccggc gcctacaggg
     5581 gatatggagc gcctcaggga ctcttcgcgc tcgactgcgc catagacgaa ctggcgcacg
     5641 agctgaagat cgacccgctg aaagtgaagg agatgaactc gatcagggag ggagagacct
     5701 ctccgatctt caagataatg ggcgagggcc gtgaaggcgt tccccagatc atgaagagct
     5761 gcaagctcca ggagtgtata gatctgggaa agaaagcgat aggatgggac gagaagaggg
     5821 gcaggcgcac gagaaggggt agcagggtca ggggtttagg ctgcgcgctg gccatgcagg
     5881 gatcgggtat acccagggtc gatatgggcg ccgcgacgct caagatgaac gaagacggct
     5941 ccttcaacct tctggt
//
LOCUS       DAOOLY010000149         5882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16231_length_5882_cov_5.336365, whole genome shotgun sequence.
ACCESSION   DAOOLY010000149 DAOOLY010000000
VERSION     DAOOLY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16231_length_5882_cov_5.336365"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLD15_08000"
     CDS             complement(<1..698)
                     /locus_tag="PLD15_08000"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPB63888.1"
                     /translation="MKVAFPAQMRRIDGLIIERGVPSMLLMENASRSVLEEVLKYLSV
                     KEVAILCGGGNNGGDGYAVGRLLLNAGKTVSVIECYRPSTPDTIFNASLFVSLGGRIH
                     RFEEGTAESLLSRADMIIDSVFGTGFHGELQGKVAELVEYGNGLKSIKLAVDIPSGVS
                     GFDGSVRGVAFRADKTVTFGAGKVGHYLHPGRDYCGEVVVRSEGFSREILDGQLDSSV
                     IDEELVLKLLPERPV"
     gene            771..1475
                     /locus_tag="PLD15_08005"
     CDS             771..1475
                     /locus_tag="PLD15_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63889.1"
                     /translation="MKRILVFLFVLISLYAFSTEEYNDLTLFIREIVESGDIDARVKM
                     EFHIIDSDGDGSSYRDILVGARLMLSGGEDLYIEFLEPEEMSEIAFVYLMREEVIFSI
                     VEGVPWKQYRWSYETGLIENLLGQFFRGLMEQSNFSWEVETLSDRSIYKILPSESRMR
                     ILGLLGGGGYLPNLMHIYLSLKRREGYFPNLEFLKITDRLEKEYLYIEFLDLQMKADK
                     QLFSDLRKSFYETFQK"
     gene            complement(1466..1807)
                     /locus_tag="PLD15_08010"
     CDS             complement(1466..1807)
                     /locus_tag="PLD15_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63890.1"
                     /translation="MSLSFNEVILYGIATSDPVSTERGGFFNIGVKRFSKVGEVLEIF
                     EVRFLKKSAGRVVTEIHAGSLLLLKGALRIYKRTHHIDLEDFVVTTDLFDRDLGIEIP
                     RIVTKLGRDYF"
     gene            1907..2314
                     /locus_tag="PLD15_08015"
     CDS             1907..2314
                     /locus_tag="PLD15_08015"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPB63891.1"
                     /translation="MSRIPRVALIAHDKKKIDLIMFVREHLDIFKNCRLFATSTTGNL
                     LKEKVKLELTSFKSGPLGGDLQIGSLIANDEIDFVIFLRDPLTAQPHEPDVTALLRVC
                     DVHNKPLATNLATAEALVMEIGRFLDSSDLGKE"
     gene            complement(2231..3502)
                     /locus_tag="PLD15_08020"
     CDS             complement(2231..3502)
                     /locus_tag="PLD15_08020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPB63892.1"
                     /translation="MVLSSTGERLKMTGIEYVAHIDMDAFFASIEQAANPCLKGRPIA
                     VTGIGKRHSVVTSASYEAKRLGVRSGMPFFQAVKLCPGLMAVKVQSRKYEYVSREIMK
                     MLSGVSPRIMVASIDEAYVDLGFYTKLEEALKSFIKFKHDLKESFGITASVGVTVNPV
                     LSKIASDYSKPNGFVVVKKGLERAFLKHVPSGDVPGIGRHTLIKLESMGYKTVGEVLE
                     EFERNPLRLYTAFGNSFLGLIQSLTKNSFSREIFFREEPPKSVGHSMTLSVDITGREL
                     VMRVANFLAARVVYRLGRYSMESAGLTVHLRYSDGKSRRLSKRLGSMISSIKDMTEIL
                     PWMIESIWKGEAVRAVGITCWNLTKKRRGQQLSLFEAKRNPLETALEIERKFGEYSIF
                     PGNILFLPEIRRVEKPSDLHYQGFCRRQVSC"
     gene            complement(3472..3711)
                     /locus_tag="PLD15_08025"
     CDS             complement(3472..3711)
                     /locus_tag="PLD15_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63893.1"
                     /translation="MAQKRLSKALVYYNEEYFIPIRFSYDGIKIFIKVITSNWIESAG
                     RERIYHFSVLTNMGAYSLEFHEALKKWYCHPPENG"
     gene            complement(3782..4150)
                     /locus_tag="PLD15_08030"
     CDS             complement(3782..4150)
                     /locus_tag="PLD15_08030"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPB63894.1"
                     /translation="MKFPDDRELVNLSRILSLKVFGVFPDIPFSFNSRLKRVLGRLVY
                     LKAAGRFKPLRIEIASSIYENEELLTGTILHELSHYYLMINGRDFSHSSQDFKKLSSE
                     LGFDIRVSAKVESSRGSENS"
     gene            complement(4197..4622)
                     /locus_tag="PLD15_08035"
     CDS             complement(4197..4622)
                     /locus_tag="PLD15_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPB63895.1"
                     /translation="MRRFRVRVNGKEYDVEIEEVSSQTQVQQTVSEAPETPVAARKTE
                     PVKKVEEVKRSEPEVDERPSQSGDNGGTNVVSPMSGTILEVFVSVGDSVSVGQKLLIL
                     EAMKMENSILAESSGMVQQVKVRKGDNIDAGETMIVLGS"
     gene            complement(4619..4969)
                     /locus_tag="PLD15_08040"
     CDS             complement(4619..4969)
                     /locus_tag="PLD15_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPB63896.1"
                     /translation="MDQYLQITIIGIVIVFLTLAILYFVFNILGRLLSRDDKKKITPR
                     VVAPSPSADSGRVGERPSQEAETVAAISAAILATIGHGNFQIKSLKPVPTVLPSAWKR
                     RDPVVYWRLRRSKN"
     gene            complement(4980..>5882)
                     /locus_tag="PLD15_08045"
     CDS             complement(4980..>5882)
                     /locus_tag="PLD15_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPB63897.1"
                     /translation="GALVHNSKSGNAHFIADNDKEAMSLVRKLLDFIPQNNAEEPDRL
                     PSPGMADEEKLRTIIPDNPKQSYDVKDIISLLVDGGEFVEVHRHYAKNMVVGFARIGG
                     RALGIVANQPAVFAGSLDINASDKAARFIRFLDAFNLPIITLVDTPGFLPGVSQEHGG
                     IIRHGAKLLYSYSEASVPKVTLILRKAYGGAYIAMGSQHLGADMVFAWPGAEIAVMGP
                     EGAANIIFRKEIESSSEPEKTREEKINEYKELFANPYVAASRGYIESVIDPARSRAEI
                     CRALDMLETKTEGRPAKKHGNIPL"
BASE COUNT         1388 a         1538 c         1374 g         1582 t
ORIGIN      
        1 acgggacgtt ccgggaggag tttcagaacc agctcctcat ctatgaccga cgaatcgagt
       61 tgtccgtcga ggatctctct ggagaagcct tcgctcctca caaccacctc gccgcagtaa
      121 tcgcgcccgg gatgaaggta atgacctacc ttcccggctc cgaaagtcac agttttatcg
      181 gccctgaaag ccactcctcg aacgcttcca tcgaagccgg aaactccgga gggaatatcc
      241 accgccagct ttatggattt cagtccgttg ccgtactcta ccaattcggc gacttttccc
      301 tgcaactctc cgtgaaagcc cgtcccgaat acggagtcta ttatcatatc ggcacgcgaa
      361 aggaggctct ctgccgtacc ctcttcgaat cggtgtatcc ttcctcctaa agagacgaag
      421 agagaagcgt tgaagatcgt atcgggcgtg gaaggacgat agcattcgat tacagagaca
      481 gtctttccgg cgttcagcag caacctcccc accgcgtaac cgtccccgcc gttgttacct
      541 ccaccgcaaa gtatcgcaac ctccttgacc gagaggtatt tcagaacttc ctcgagtacg
      601 ctcctcgaag cattttccat gagcaacata gagggaaccc ctctttcgat gatcagtccg
      661 tctattcttc tcatctgcgc tggaaatgca actttcaaag tatcacctca taaagatctt
      721 atcattttta cccccgattg acatgttata atgtgccgag gtgaaagcga ttgaagcgaa
      781 tcctggtttt tctctttgtg ctcatttccc tgtacgcctt ttcgaccgaa gaatacaacg
      841 atctgacact cttcataagg gagatagttg aaagcggcga catagatgca cgcgtcaaga
      901 tggagtttca tatcatcgat tccgacggcg atggttcgag ttatcgagat attctggtcg
      961 gtgcccgttt aatgctgagc gggggcgagg atctgtacat tgagtttctc gagccggaag
     1021 aaatgagtga aatagctttc gtatacctta tgagggaaga ggttatcttc agtatcgtag
     1081 agggagttcc atggaagcag taccgctgga gttatgaaac gggactgatc gagaacctgc
     1141 tgggacagtt tttcaggggt ctcatggagc aatccaattt cagctgggaa gtcgaaaccc
     1201 tttccgatag aagtatttac aaaatactcc cttcggaatc cagaatgagg atactcggtc
     1261 ttctcggcgg cggcggttac ctccccaacc tcatgcacat atatctttcg ttgaagagaa
     1321 gggagggtta ttttccgaac ctcgagttct tgaagataac cgacagactg gagaaggagt
     1381 atctgtatat cgaatttttg gatctgcaga tgaaagccga caagcagttg ttttccgatc
     1441 tcagaaagag cttctacgaa acctttcaga agtaatctct cccgagcttg gtgactattc
     1501 tgggtatctc tatacccagg tctctgtcga agagatcggt ggtaacgacg aaatcttcga
     1561 ggtcgatgtg gtgggtcctt ttataaattc tcagtgctcc cttcaggagg aggaggctgc
     1621 cggcatgtat ttccgtgacc accctcccgg cgctcttctt caagaaacga acctcgaata
     1681 tctccagaac ctccccgact ttagagaatc tcttcacccc tatattgaaa aagccgccgc
     1741 gctctgtaga tacaggatcg gacgtggcta tgccataaag gatgacttcg ttaaaagata
     1801 aagacaaagg tcacctcgat tcaacaactc gataactcga tctgcctccc ggtcgctaag
     1861 ggtcgcgttg tggtatcatc ttttcaggaa atcaaaggag gtaattatga gcaggattcc
     1921 aagagttgcc ctcatagccc acgataaaaa gaaaatcgat ctcataatgt tcgtcagaga
     1981 acatctggat atcttcaaaa actgccggct cttcgccacc agtacgaccg gaaaccttct
     2041 gaaagagaaa gtcaaactag agctgacttc cttcaaatcc ggtccactcg gcggagatct
     2101 tcagatcggt tctttaattg ccaacgacga gatagatttc gttatctttc tgagagatcc
     2161 tctcacggca cagccccacg aaccggacgt gaccgccctg ctcagagtct gcgatgttca
     2221 caacaaacct ttagcaacta acctggcgac ggcagaagcc ctggtaatgg agatcggaag
     2281 gtttctcgac tcttctgatc tcgggaagga atagtatatt accgggaaag atcgaatact
     2341 ctccaaactt tctttcgatc tccagtgccg tttccagcgg gtttctcttc gcttcgaaaa
     2401 gtgagagctg ctgtccgcgc ctctttttgg tgaggttcca gcaggtaatg ccgacggccc
     2461 tgacggcctc tcccttccag atcgattcga tcatccacgg caggatttcg gtcatatcct
     2521 ttatggagga gatcatggaa ccgagtcttt tcgaaagtct gcgcgacttt ccgtcggagt
     2581 acctaagatg aacggtgagt cccgcacttt ccatcgagta cctcccgagt ctgtaaacaa
     2641 ctctggcggc caggaagttg gctactctca tcaccagttc ccggccggtg atatccacag
     2701 aaagagtcat ggaatggccg acggatttgg gtggttcctc tctgaaaaag atctcccgtg
     2761 aaaaactatt ttttgtgaga ctctgaatta gacccagaaa gctattcccg aaagcggtgt
     2821 agagtcttaa gggatttctt tcgaactcct cgagaacttc tccgaccgtt ttgtaaccca
     2881 tcgactccag cttgataagc gtatgcctcc cgatgccggg gacgtcgccc gaaggtacat
     2941 gcttaagaaa agccctttcc agacccttct tcacgacgac aaaaccgttc ggcttcgaat
     3001 aatcgctcgc gattttggaa agcaccggat tgaccgtgac ccccacagag gcggttatcc
     3061 cgaaactttc tttcaaatcg tgtttgaatt ttatgaaact cttcagagcc tcctcaagct
     3121 ttgtatagaa accgagatcg acgtaagcct cgtctatact ggccaccatt attctcgggg
     3181 aaacgccgct tagcattttc atgatctctc tggagacgta ttcgtacttc ctggattgca
     3241 ctttcaccgc catcaaacct ggacagagct taacggcctg aaagaagggc attcccgatc
     3301 tcactccgag tcttttggct tcgtaactgg ccgaagtcac taccgaatgg cgtttgccga
     3361 ttccggtgac ggcgatcggc cggcctttca agcaagggtt ggccgcctgc tctatactcg
     3421 caaagaaagc gtccatatct atgtgagcca cgtactctat gcccgtcatt ttcaaccgtt
     3481 ctccggtgga tgacagtacc attttttcag tgcttcgtgg aactccagac tgtaggctcc
     3541 catgttggtg aggaccgaga agtggtatat tctctctcta cccgcggact ctatccagtt
     3601 agaagtgatg acctttataa aaatttttat accatcatag ctgaatctta taggtatgaa
     3661 gtattcttca ttgtaatata caagcgcttt ggatagcctc ttttgagcca taattccatc
     3721 accgttataa ttatttatac caaatggtat aaataattat agcagatgat ttccggaaaa
     3781 atcaactatt ttccgatcct ctggaagatt cgacttttgc agatactctt atgtcgaaac
     3841 ccagttcact ggagagtttc ttgaagtctt gcgaggaatg cgagaagtct ctaccgttta
     3901 tcatgagata ataatgcgag agctcgtgca gtatcgtgcc tgtgagaagc tcttcgtttt
     3961 cgtaaatcga ggaggctatc tctattctca acggcttgaa ccgaccggca gccttcaggt
     4021 aaacaagcct gccgagaact ctttttagtc tcgagttgaa cgaaaaaggt atatcgggaa
     4081 agactccgaa aacctttaag gagaggattc ttgacaggtt gaccagttct ctgtcatctg
     4141 gaaacttcat aacgcttgaa agacttcccg aatcataaag gcggaaagat ccgcctttat
     4201 gatccgagaa ctatcatagt ctctccggca tctatattgt cgcccttgcg gaccttcacc
     4261 tgctggacca ttccggaact ctccgccagg atgctgttct ccattttcat ggcctccagt
     4321 attaacagtt tctggccgac gctcacgctg tctccaacgg agacaaaaac ctcgaggatc
     4381 gttccagaca tcggcgagac aacgttagtc cctccgttgt cgcccgattg agaaggtctc
     4441 tcatcgacct ccggttcgga tcttttcact tcttcgacct ttttcacggg ttccgttttt
     4501 ctggcagcta caggtgtctc cggagcttcg ctcaccgtct gttgaacttg tgtctgcgaa
     4561 gatacttctt cgatttctac gtcgtattcc tttccattaa ccctaaccct gaaccttctc
     4621 aattcttgct cctccttagt ctccagtaaa ccacagggtc ccgtcttttc caggccgatg
     4681 gcagaacggt cgggaccggc ttcaatgatt ttatctgaaa gtttccatgt ccgatggttg
     4741 ccaatatagc cgcacttata gcggcaacgg tttcggcttc ctgcgaaggt ctctctccga
     4801 ctcttccgga atcagccgac ggagagggtg cgacgactct gggtgtaatc ttctttttat
     4861 cgtccctgga taaaagtcta cccagtatgt tgaatacgaa atagagaatc gcaagcgtta
     4921 ggaagacaat tacgatacct atgatcgtta tctgaagata ctgatccatc gctcacctct
     4981 taaaggggta tatttccgtg ctttttggcc ggtcgcccct cggtctttgt ttcgagcata
     5041 tccagagccc tgcatatctc ggccctgctt ctcgccgggt ctatgacgga ctctatgtat
     5101 cctctggaag cggcgacgta gggattggcg aagagttctt tgtactcgtt tatcttctct
     5161 tccctggtct tctcgggctc cgaagagctt tcgatttcct tcctgaatat gatgttcgcc
     5221 gccccttcgg gccccatgac ggctatctcg gcaccgggcc aggcgaaaac catgtcggct
     5281 cccagatgtt gactgcccat ggcgatgtag gcaccgccgt aagcctttct caggatgagc
     5341 gtcacctttg gcaccgaggc ttcgctatag gagtaaagga gcttggcgcc gtgccttatt
     5401 atccctccgt gttcctggga tacgcccgga aggaagcccg gcgtatcgac gagcgtgatt
     5461 atgggaagat tgaaggcgtc caggaacctt atgaacctcg ccgccttatc ggaggcgttt
     5521 atatcaagcg agccggcgaa gacggccggt tggttggcga ctattcccag ggccctcccg
     5581 ccgatccttg caaaaccgac caccatgttt ttcgcgtaat gcctgtggac ttcgacaaac
     5641 tccccaccgt ccaccaggag cgatattata tcctttacat cgtacgattg cttcgggttg
     5701 tcgggtatta tggtcctcag cttctcttcg tcggccatcc cgggtgaagg tagtctatcg
     5761 ggttcttcgg cgttgttctg gggaatgaag tctagcagct ttctgaccag agacatggct
     5821 tctttatcgt tatcggctat gaaatgtgcg ttcccgcttt tgctgttgtg gacaagtgca
     5881 cc
//
LOCUS       DAOOLY010000150         5871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16281_length_5871_cov_5.185695, whole genome shotgun sequence.
ACCESSION   DAOOLY010000150 DAOOLY010000000
VERSION     DAOOLY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16281_length_5871_cov_5.185695"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..936
                     /locus_tag="PLD15_08050"
     CDS             4..936
                     /locus_tag="PLD15_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB63898.1"
                     /translation="MTHVYGVVPSRRLGRSLGVSTIPFKTCNYSCIYCQLGRTTNMTN
                     TRTSFYPPGEIINQVRDFVNEKGEESFDVVTVVGEGEPTLYRPLDHLAGAIRELTSKP
                     LVLITNGSLLFDADLRDEISGFDIVMPTLDAYDRESFRRINRPFGKLEYEEVYEGLVD
                     FSGEFAGEVWLEVMLVKGYNDDERSLALLKERLSRIRARRVYINVPARPPAEDGVTIP
                     DNATLKLARELLGAASIENLPVSNFLSLESDAESTVIEIIKRHPMSKEDIESLLFSRF
                     GPGSAEGVLEKLSSREDVERREYAGRIFYRYRSV"
     gene            1117..2043
                     /locus_tag="PLD15_08055"
     CDS             1117..2043
                     /locus_tag="PLD15_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPB63899.1"
                     /translation="MGRVAVIGELLIDLISDTQVSELSRAKSFNRFFAGSPGNLVFNL
                     HDLGVDSAILSRVGDDSFGRAYIDLLAKRGIDVSYVQFDRRRHTSFVIVSRSDSTPQF
                     LALRGADCMLEPPVEIDRFLDGVQFLHLTSWPLSLSPARETTVSVVQKAAERGIKISL
                     DPNYREVIWECGHDGPAFIKKMLRYIHIVKPSDDDARHIFGEMQPREYLDLFHEFGAK
                     NVILTLGKRGAVVSDGFRIEEIAPCARRVVDTTGAGDAFWSGLYRGLLERKDIFEAAR
                     YGNMCAAFRIEHEGKDTQLPAIEELKTIYERC"
     gene            2047..3495
                     /locus_tag="PLD15_08060"
     CDS             2047..3495
                     /locus_tag="PLD15_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB63900.1"
                     /translation="MRVAFINPQGNFDSRDSYWTTHPDFGGQLVYVKELAVAMSGLGI
                     NCDIITRRITDPDWPEFAGDMDSYPGVENVRIIRIPFGPEGFLKKEELWPHLDQFRKG
                     VIEFYRQDRTMPSFITAHYGDGGLVGAMLFKDTGIPFSFTAHSLGAQKLEKLLQTGDD
                     RGELEEKYNFSYRIAAERLAMKYSAVNFVSTSMERYQQYSHRLYRDFVNIGDDSRFRV
                     VPPGVNTSIFKTESTDLDAHIEEKLNSALKGFVEPSRHSLPLIIVSSRLEPKKNHTGL
                     VRAFSTDTALNEASNLLIVVRGMENPYDEYEKTDDPERVVLSEIIEIVRRNGVEKKVL
                     FLNIESQLELAALYRVAGKLRSIFALTSLYEPFGLAPIEAMACGLPVVATSNGGPSES
                     LNENGVEYGVLVDPADTQDISRGLKRLINCDQAVWKSFRERAMDRVMDKYTWAATAKG
                     YLDAIKELIKSTYPEPEIPDYIYTGIDVPFIE"
     gene            3712..5277
                     /locus_tag="PLD15_08065"
     CDS             3712..5277
                     /locus_tag="PLD15_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPB63901.1"
                     /translation="MQTYKGWAFVVSSGLLFYFMIFREFRGKAKANVLLTRQKDFSNS
                     VLNSAGVYVAVIDDRGTIARINEKFERLFSSSGGEIIGKSIYDIFLNPSTASGLRSAV
                     DDIRDGDGEQVFESGCPVPGNTLHIRWSLRFLASWKEETDYFVLTGVDITKVVESERS
                     ATKRLNNIQALHEIDLAVSYNVELEKMLDVFLEKSISRLEVDGADIYLLSEDNKLRFT
                     HGMGSISGKSLPVDLPLEGTVPGRVVLTGEPYSGPLRPDSGVNCPRLEMIKGMKISEY
                     HAVPLQTRGKTLGVLEVFSVDERERDSEWHDFLITLAGQGSLAIDVAIMIESLKASNK
                     MIKHAYDQTLEGLAMALELRDIEMEGHSRRVTELSMALAREMGLSDGELESFYRGALL
                     HDIGKISVPDEILFKKGPLDESEWEIMKAHTVYARKLLSQIDYLVPSLDIPYCHHERW
                     DGSGYPQGLKGEEIPLSARIFAIVDVYDALTSERPYKHAWSEEEALKYLRENSGKLFD
                     ERIVDRFLKIVKS"
     gene            5305..>5871
                     /locus_tag="PLD15_08070"
     CDS             5305..>5871
                     /locus_tag="PLD15_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB63902.1"
                     /translation="MQYDFDRKIERRGTNCLKWDHADWFFGTNDLLPMWVADMDFEAP
                     EPVLEAIRERAGHGAFGYTVKPESYHEALVEWMKKRHRWEIKKEWLAHAPGVVPAVHL
                     SIMAFSHPGDTVLVNSPVYYPFYSAVKNTGRQLLLSPLKSEEGRYEMDFEDIERRIDS
                     RTRLFILSNPHNPVGRVWKIKELERLGEI"
BASE COUNT         1469 a         1404 c         1666 g         1332 t
ORIGIN      
        1 ttcatgactc acgtgtacgg tgttgtgcct tcgcggagat tgggcaggtc gctgggtgtg
       61 tcaacgattc ccttcaagac ctgcaattat tcatgtattt attgccagct cgggagaacg
      121 acaaatatga ctaacacccg cacgagtttc taccctcccg gggagataat aaaccaggtc
      181 agggatttcg tgaacgagaa gggcgaggag agtttcgacg ttgtaactgt cgtgggcgag
      241 ggcgagccca cgctgtacag accgctcgat catctggcgg gggcgatcag ggagttgact
      301 tccaaacccc tcgtgctgat caccaacgga tcgcttctct tcgacgcaga cctcagagac
      361 gagatctccg gtttcgacat cgtgatgccg acactggacg cttacgaccg ggagagtttc
      421 agacgaatca acaggccgtt cggaaagctc gagtacgaag aggtttacga gggtctggtg
      481 gatttctctg gagagttcgc cggagaggta tggctggagg tcatgctcgt aaagggttat
      541 aacgacgacg aaaggtccct tgcactgttg aaagagcggc tttccagaat tagagcgagg
      601 agagtgtata taaacgttcc cgccaggcca ccggccgaag atggtgtcac aataccggat
      661 aacgccactt tgaaactcgc acgtgaattg ctgggcgctg cgagtatcga aaaccttcca
      721 gtcagcaatt ttctatcgct cgaaagcgat gcggagagca ccgttatcga gatcataaag
      781 cgccacccca tgtcgaaaga agatatagaa agcctcctct tttcgcggtt cggtccgggt
      841 tcagccgaag gggttttaga aaagttatcc tctagagaag atgtggaaag gcgcgaatac
      901 gcagggagaa tcttctacag atacaggtcg gtctgatcga aggccggaaa taaatctcgc
      961 ctcgcgattt ttagatcttg ccgtcacagg acgaaaaagt cctcttccac ttctgcccac
     1021 tgtctcctgt gttgtgcctc gggtgaggta ctgcaaaggc tttccgtatt tgtcggtcat
     1081 aatgataaac ttgaatcaag gaaggagggc tttccgttgg gaagagttgc ggtgatcggc
     1141 gagctactca tagatctgat ttccgatacg caggtcagcg aactttcacg ggcgaagagc
     1201 ttcaacaggt tcttcgcggg ctcgccgggc aacctcgttt tcaatcttca tgatctcggc
     1261 gttgattccg ctatcctttc cagagtcggg gacgactcct tcggccgggc ctacatcgat
     1321 cttctcgcaa agagggggat cgatgtctca tatgttcaat ttgacagaag gaggcacact
     1381 tcgttcgtga tagtatcgcg ttccgactcg actccgcaat ttttggccct gcgcggcgcg
     1441 gattgtatgc tggagcctcc cgtggagata gatcggtttc tcgacggtgt gcagtttctt
     1501 catctcactt cctggccgct atcactttca ccggcccgcg agacgaccgt ctcggtagtt
     1561 cagaaagctg cggagagagg gataaagatc tcgctcgatc ccaactatcg cgaagttatc
     1621 tgggagtgcg gacacgacgg acctgccttc atcaagaaga tgttgagata tatccacatc
     1681 gtcaagccct cagacgacga cgcgcggcat atattcgggg agatgcaacc gcgagagtac
     1741 ttagatctct ttcacgaatt tggagcgaaa aacgtgatac tgacactcgg gaaacgtggt
     1801 gcggttgtct ccgacggttt caggattgaa gagatcgctc cctgcgccag aagggtcgta
     1861 gataccaccg gggccgggga tgccttctgg tcgggcctgt acagagggct gctcgagagg
     1921 aaagatatct tcgaagcggc ccgttatgga aacatgtgcg ccgcctttag aatagaacac
     1981 gaaggaaaag atacgcaact ccctgcgatc gaagagctaa agacgattta tgagaggtgt
     2041 tgaagcatga gagtagcatt tatcaatcca caggggaatt tcgattcgcg cgacagttac
     2101 tggaccactc atcccgattt tgggggacag ctcgtatatg tcaaggaact ggccgtcgcg
     2161 atgtccggac tgggtatcaa ctgcgacata atcacccgaa gaatcactga tccagattgg
     2221 ccggagttcg caggcgatat ggattcgtat ccgggtgtgg aaaatgtgag aataataaga
     2281 atcccgttcg ggcccgaagg cttcctgaaa aaggaagagc tctggccgca tctggaccag
     2341 ttcagaaagg gggtaattga gttctaccgt caggacagaa ctatgcccag cttcataacc
     2401 gcccattacg gcgacggcgg cctggtcggg gcgatgcttt tcaaggatac aggcatcccc
     2461 ttttccttca ctgcccattc gctcggcgca cagaagctcg aaaagcttct tcagaccggc
     2521 gatgacaggg gcgagcttga agagaagtac aacttttcgt accggatagc ggccgagagg
     2581 ctcgcaatga agtattcggc cgtcaacttc gtctccactt cgatggaacg ttaccagcag
     2641 tacagccaca ggctttacag ggactttgtg aatatcgggg atgactccag gttcagagtc
     2701 gtgccccccg gtgtgaacac ttcgatcttc aagacggaat ccaccgatct ggacgcccat
     2761 atcgaggaga agctgaattc agctttgaaa gggtttgtag aaccttcaag acactctctg
     2821 cccttgatca tcgtctcgag cagactggaa cccaagaaaa accacaccgg cctcgtaagg
     2881 gccttctcaa ccgatactgc attgaatgag gccagcaatc tcttgatagt cgtgagggga
     2941 atggaaaatc cgtacgatga gtacgaaaag accgatgatc ccgaaagagt tgttctttcc
     3001 gagattatcg agatcgtcag aagaaacggc gttgagaaaa aggttctctt tctcaacatc
     3061 gaaagccagc tagagttggc cgctctgtac agagttgccg gcaagctgag atcgatcttt
     3121 gcgctgactt ctctgtacga accgttcggc ctggcgccga tcgaagccat ggcctgcggg
     3181 ctaccggtag ttgccacttc gaacggcggt ccatccgaga gcttgaatga aaacggagtc
     3241 gagtacgggg ttctggtcga tccggccgat acgcaggata tttcccgcgg gttgaagagg
     3301 cttataaact gcgatcaggc tgtctggaaa tccttcaggg aacgagccat ggacagagtg
     3361 atggacaaat acacctgggc agccacggca aaggggtatc tcgatgcgat caaagagctt
     3421 atcaaaagca cgtatcccga gccggagatt cccgactata tatataccgg gatcgatgta
     3481 cccttcatcg agtgagaagg ctaaccgttc gataaccggg ccaatatgtt tgccagtcag
     3541 gaagggaggc gtgccgctta accctttgaa cggggtcctt ggcggtgagt tgctgtgaag
     3601 aatccggcct tgaaggttgc attcgcatat attgtatttg gagccttgtg gattttgttc
     3661 tcggatatgc tggtcaacgc gttttttccg gataaggctt ctttatccac cgtgcagacc
     3721 tataagggct gggcctttgt agtctccagc ggtcttctct tttacttcat gatcttcagg
     3781 gagtttcgtg gaaaagcgaa ggcgaacgtg ctgctgacga ggcagaagga cttttccaat
     3841 tccgttctca acagcgccgg ggtctatgta gcggtaattg acgacagggg aacaattgcg
     3901 agaatcaacg agaagttcga aaggcttttc tcctcttccg gcggagaaat catcgggaaa
     3961 agcatatacg atattttctt gaatccctcg acggcgagcg gtttgagatc ggccgtagat
     4021 gatatccgtg atggagacgg cgaacaggtt ttcgaaagcg gctgcccggt tcccgggaat
     4081 actctgcata ttcgctggtc gttgcgcttt ctcgcctcct ggaaggaaga gaccgattat
     4141 ttcgttctca ccggagtcga tataaccaaa gtcgtcgaat cggagaggag cgcaactaaa
     4201 agattgaaca atattcaggc tcttcacgag atagatcttg cggtcagtta caacgtagag
     4261 ctcgagaaga tgctggacgt ctttctggag aagtccatat ccaggctgga agtggatggc
     4321 gccgatattt acttgctgag tgaggacaat aaattgagat tcacgcatgg aatgggatcg
     4381 atatccggca aaagtctccc tgtcgatctt ccgcttgaag gtaccgttcc cgggagggtc
     4441 gtgctcacgg gcgagcctta ttcggggccg ttgcgccccg attcaggcgt aaactgcccc
     4501 agacttgaga tgataaaagg gatgaagata tccgaatacc acgcagttcc ccttcagacg
     4561 cgcggcaaga ccctgggagt gctggaggtc ttcagtgttg atgaaaggga gcgtgacagc
     4621 gaatggcatg actttctgat cacactcgcc gggcaggggt cactggcaat agatgtggct
     4681 atcatgatag agagccttaa agcgagcaac aagatgatca agcacgctta cgatcaaacg
     4741 ctagaaggcc tcgcgatggc cctcgagttg agagatatag agatggaggg gcattcgcgc
     4801 agagtgacgg aactgtccat ggccctcgcc agagagatgg ggctgagcga tggagagctc
     4861 gagagctttt acaggggcgc gcttctgcac gatatcggaa agatttccgt gccggacgag
     4921 atactgttca aaaaaggtcc gctggatgaa agcgagtggg agataatgaa agcgcacaca
     4981 gtctatgccc gtaagctcct ttctcagatc gactacctgg tgccgtcgct ggatataccc
     5041 tactgccatc acgaacgctg ggacggttcc ggttatcccc aaggactgaa aggtgaagag
     5101 attcctcttt cggccaggat tttcgcgatc gtggatgttt acgacgcgct gacttcggag
     5161 aggccgtata aacacgcctg gtccgaagaa gaggcgctga agtatttgcg agagaattcg
     5221 gggaaattat tcgacgaaag gatcgttgat cgcttcctga aaatagtaaa aagctgaaga
     5281 attataaaaa cggaggttgc gcttgtgcag tacgatttcg acagaaaaat agaacgcagg
     5341 ggtacgaact gtcttaaatg ggaccacgcg gactggtttt tcggcacgaa cgatcttctg
     5401 ccgatgtggg tggcggacat ggatttcgaa gcgcccgaac cggtgctgga ggcaatcagg
     5461 gagagggcag ggcacggggc ctttggatat accgttaaac cggaaagcta ccacgaggcc
     5521 cttgtcgagt ggatgaaaaa aaggcacaga tgggagatta agaaggaatg gctggcgcac
     5581 gctcccggag tggttcccgc agttcatctg agcatcatgg ccttttccca tcccggcgat
     5641 acagttctgg tcaacagccc ggtctattac cccttctaca gcgccgtaaa gaacaccggc
     5701 cgacagcttc ttttgagtcc tctcaaaagc gaggaaggcc gttacgaaat ggatttcgaa
     5761 gatatcgaga gaagaataga ctccaggaca agactcttca tactcagcaa cccccacaat
     5821 ccggtgggca gggtctggaa aataaaagaa ctggaaagac tgggagagat c
//
LOCUS       DAOOLY010000151         5844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16379_length_5844_cov_4.851097, whole genome shotgun sequence.
ACCESSION   DAOOLY010000151 DAOOLY010000000
VERSION     DAOOLY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16379_length_5844_cov_4.851097"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLD15_08075"
     CDS             <1..580
                     /locus_tag="PLD15_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB63903.1"
                     /translation="RDVIEALRQPLEDGVITVARAKLTATYPARFMMVAAQNPCPCGW
                     YGDKTRECTCSWYEIQRYNRKISGPMEDRIDIFVDMPRLDFDKYMETSIAESSASVRE
                     RVIRARNRQLERYRGMNLFSNSQLGHSLLKEYVNLDPTSRSLLSGAVDKMKLTARSID
                     RILKVALTIADLEENKVLARHIAEAIQYRKKT"
     gene            969..3389
                     /locus_tag="PLD15_08080"
     CDS             969..3389
                     /locus_tag="PLD15_08080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPB63904.1"
                     /translation="MKEYVVKGMTCAACVRAVEKAASKAEGVSGPVVSLATEKLTFEL
                     SGGFDEEKLFNLVKKAGYELEEVRKERIVTLEIDGMTCAACVSAVEKAIAKLDGVSEV
                     SVNLAAEKATVGYDPDRLRISSIKEAIEKAGYRASAFTNADSEQSRLKKEAAIRSYKQ
                     KFLVSIVFALPLLIIAMGHMIGLKLPSFIDPHMNPLNFALAQLFLTIPIIIAGKDFYL
                     KGIPNLFRGHPNMDTLVGLGTGAAFIYGVFATVQIASGNHNYAGDLYFESAGVIIALI
                     SLGKYFENLSKGKTSEAIRKLMNLAPKKALVKREERFVEIPLEEVEAGDVLLVKAGMS
                     IPVDGIVIDGTTSVDQSMLTGESIPVDITEGSKVTGGTMNINGAIEIKASRVGSDTVL
                     SKIIKMVEEAQASKAPIARLADTVSGYFVPFVLVVALVAFVTWIALGYGFVFSMSMTI
                     AVLVIACPCALGLATPTAIMVATGRGAGMGILFKNGEALETTHKVDAVIFDKTGTITR
                     GKPELVDFKVLNGFAEEESIRLVASIASRSSHPLDRAILDSYDGDLLQVSDFQAVAGG
                     GISATVNGGKVKLGNPGFIGTTGSEVDEFVRSLSELGKTPVVASYDGKVMAVLGIADV
                     IKDSSARAIERLKRSGVETYMMTGDNERTARAIAAQVGVDNVIAEVMPQHKAGKVGQL
                     KERGFTVAMVGDGINDSPALAAADVGIAIGSGTDIAIEAADVVLMKDDLNDVVNSINL
                     SKATIRNIKQNLFWAFFYNTIGIPVAAGLFYVAFGLRLNPMIAGAAMAFSSVSVVTNA
                     LRLKKIKI"
     gene            3403..3609
                     /locus_tag="PLD15_08085"
     CDS             3403..3609
                     /locus_tag="PLD15_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPB63905.1"
                     /translation="MKLIIEGMSCNHCKMRVEKALSSIEGVKKARVDLDDGSAVVESS
                     VELDREFLREVIDEAGYVLKEIKD"
     gene            3611..3895
                     /locus_tag="PLD15_08090"
     CDS             3611..3895
                     /locus_tag="PLD15_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HPB63906.1"
                     /translation="MENHSHHHKHDEALKILKTARGQIDGIIRMIEDERYCIDISTQL
                     LAVVSLLKKANTSVVNRHIETCVREAVKTGEVDEKIKELETLMKYIEKSL"
     gene            complement(3913..4230)
                     /locus_tag="PLD15_08095"
     CDS             complement(3913..4230)
                     /locus_tag="PLD15_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63907.1"
                     /translation="MTGKVERLESIRRTLAMLLDDVQWKLDDVVLTIGDLIDEEKKLS
                     LDTLKNLGMLKRERRDMLSIYMKAIEDRNEVVKRMVENLWDLPENNLKEALEKLHELL
                     KDE"
     gene            complement(4279..5496)
                     /locus_tag="PLD15_08100"
     CDS             complement(4279..5496)
                     /locus_tag="PLD15_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPB63908.1"
                     /translation="MTDLKKAWLVVYSGLGVNLTLGVLYSWSIISAALIDNYGWSATQ
                     TQIPYMLASAVFALTMVPGGKLQDRLGPKPVLLISSVLAGLGFILSGLNLTVAGLTLF
                     FGVVFGLAMGFGYASPTPAAVKWFHPEHRGLISGIVVSGFGLAPVYIAPLTSSLIGLL
                     GLPATLITLGIIFFLVVFSLSFTISNPPPGFQRIELKRRPARAHQITAKDYKVREMVK
                     TVQFYLLWTMFFFGTFAGLLIIGQMSKIGLEQASISNGFLLVVVYAIFNFIGRVTWGT
                     ISDYLGRTITLLTMFLIQAVVYFSFAALTSPVALLIGKSLVGFTFGGMLAIFPVITAD
                     FYGMKNLGVNYGVMITAWGVGGILGPLLGGIARDITGGYGISYLVSAILSIAGAAISL
                     LVRHPDRESRSLK"
     gene            complement(5612..>5844)
                     /locus_tag="PLD15_08105"
     CDS             complement(5612..>5844)
                     /locus_tag="PLD15_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63909.1"
                     /translation="SFGVLYEPFLWQSSIEKSGECLMLWKRGVKLARNAAFRRVEIQG
                     STLLIKSKGIWKIVCIQYFVPVSFKSLLSKKK"
BASE COUNT         1586 a         1385 c         1543 g         1330 t
ORIGIN      
        1 acgagatgtc atagaagctc tcaggcaacc tctggaagat ggagtcataa ccgtagcacg
       61 ggccaagctc acggcaactt atccggccag attcatgatg gtagcggcgc aaaacccctg
      121 tccctgtggc tggtacggag acaagaccag ggaatgtaca tgctcctggt acgaaataca
      181 gagatacaac agaaagatct ccggtcccat ggaggacagg atagatatat tcgtcgatat
      241 gccccgcctg gatttcgata aatatatgga aacctctatc gctgaaagct ccgccagcgt
      301 aagggagaga gttatcaggg cgcgcaacag gcaactggag agatacagag gaatgaatct
      361 cttctcaaac agccagcttg gacactccct tctgaaggaa tacgtgaatc tcgacccgac
      421 atcgaggtct ttactgtcgg gcgcggtgga taagatgaaa ttgacggccc ggtctataga
      481 cagaatactg aaagttgccc tgaccatagc cgatcttgaa gaaaacaagg tactcgcccg
      541 gcacatcgcc gaggcgatac agtacaggaa gaaaacgtga taggttgttc gtaaaacacg
      601 agaaaaagag tattcgagag gttcgcttcg ctcacgagaa gacgagaatc ctcttaagaa
      661 ccgttgttgg ttgtacgcgg tgcgttgtgg gcaagggcat cgagtaggag aggggtcagg
      721 catgacggaa agagagcaag actgtcatct cgtagttctc ttatactgga tttcatctct
      781 tcagtctctc cctctctcct tttctcgtgc tctttaacta acaacgaaga gccgctcttc
      841 gcagccagtt ttctttctct ggtacatata ccagtctatt gaaatctgat aaaaaatgtt
      901 gtattattac aatatcccct ccccccgggg agtggttaat gtagatcgtc tccagactgg
      961 aggttgttat gaaagagtat gttgtaaaag gcatgacctg cgcggcctgt gtaagggcag
     1021 ttgagaaggc tgcatcgaag gcggaggggg tgtccggtcc ggtagtcagc ctcgcgacgg
     1081 aaaagctgac attcgaactt tcgggcggct tcgacgagga gaagctcttc aatcttgtga
     1141 agaaggccgg ttatgagctc gaagaggtgc gcaaggagag aatagttaca cttgagatag
     1201 acggcatgac ctgtgcggcc tgcgtttcgg ctgtggaaaa ggctatcgcg aagctagacg
     1261 gtgtgagcga ggtttccgta aatctggcgg ccgagaaggc aaccgtcggg tacgatcccg
     1321 ataggctgag gatatcttca ataaaggagg caatcgaaaa ggctgggtac cgggcaagtg
     1381 cctttacaaa cgccgattcc gagcagtcca gacttaaaaa agaagccgct atcaggagct
     1441 ataaacagaa atttctcgtt tcgatcgtct tcgcccttcc gctcctgatt atcgcgatgg
     1501 gtcatatgat aggattgaaa ctgccttctt tcatcgatcc tcacatgaat cccttgaact
     1561 tcgctctcgc ccagctattt ctcactatcc ctataataat cgccgggaaa gatttctacc
     1621 tcaagggaat tccaaatctc ttcaggggcc atcccaatat ggatacgctg gtaggtctcg
     1681 gaaccggagc agcctttatc tacggtgttt ttgcgaccgt acagatagct tctggcaacc
     1741 acaattatgc cggcgatctc tacttcgaat cggccggagt tataatcgca ctaatttctc
     1801 tgggaaagta cttcgagaac ctatcgaaag gaaagacttc ggaagccata agaaagctga
     1861 tgaatctggc gccaaagaag gctctggtga aacgtgaaga gaggttcgtc gagattcctc
     1921 tcgaagaagt cgaagcagga gatgttttgc tggtcaaggc gggtatgtcg attcccgtgg
     1981 atggaatcgt tatcgacgga accacttcgg tagatcaatc gatgctgacg ggagaatcga
     2041 tacccgtaga tatcacagaa ggctcgaaag taaccggcgg tacgatgaac ataaacggcg
     2101 caatagagat aaaggcctct cgcgtgggaa gtgacaccgt tctatcgaag attataaaga
     2161 tggtcgaaga ggcacaagcc tcgaaagctc cgatcgcacg ccttgcagat acggttagcg
     2221 gttacttcgt tcccttcgtt ctggtggttg ccctggtcgc ttttgtaacc tggatcgctc
     2281 tgggttacgg cttcgtcttt tccatgtcga tgacgatcgc cgtactggtt atcgcctgcc
     2341 cctgtgccct tggactggca acgccgacgg cgataatggt cgcaacgggc cgtggtgcgg
     2401 gaatgggaat actcttcaag aacggtgagg ctcttgagac gacccataaa gtcgatgccg
     2461 ttattttcga caaaaccggc acgatcacca gagggaagcc ggagctggtc gatttcaagg
     2521 tattgaacgg cttcgccgaa gaggagtcca tcagattggt cgcaagcata gccagcagaa
     2581 gttcccatcc gttggacagg gcgatactcg atagctacga cggcgatttg ctgcaagtat
     2641 cggattttca ggccgtcgcc ggaggaggaa taagcgccac agtaaacggc gggaaggtga
     2701 agctggggaa tcccggtttt attgggacca caggaagcga ggtcgatgaa ttcgttcgct
     2761 ccttatccga actcggaaag acgccagtcg tcgcgtctta cgatgggaaa gtaatggccg
     2821 ttctgggaat cgccgacgta ataaaggatt cgtcggccag agccatagaa aggctgaaaa
     2881 gatctggtgt ggagacctat atgatgaccg gagacaacga aagaacggcc agagccatag
     2941 cggcacaggt tggtgtagac aacgtaatcg cggaagttat gcctcagcac aaagctggga
     3001 aggtcgggca attgaaggag agaggcttca ccgtcgccat ggtgggggac ggaataaacg
     3061 attcgccggc gcttgcggca gccgatgtcg gaatcgctat aggctccggg acagatatcg
     3121 cgatagaagc tgccgatgta gttctcatga aagacgatct caacgacgtg gtgaactcaa
     3181 taaacctttc aaaggcgacg ataaggaata tcaagcagaa cctgttctgg gccttcttct
     3241 acaacacgat aggtattccc gtagcagccg ggctcttcta cgtagccttc ggtctaagat
     3301 tgaatcccat gatagccggt gcggccatgg ccttctccag cgtcagtgtc gtgacgaatg
     3361 cgctgagact gaagaaaatt aaaatataaa ggaggagaca agatgaaact gataatcgaa
     3421 ggaatgagct gtaaccattg caagatgaga gtggaaaagg ctttaagttc tattgagggc
     3481 gtgaaaaaag ctagagtcga tctggacgat ggatcggctg ttgtcgaaag ctccgtggag
     3541 ctagacagag aatttctgag agaagtgata gacgaggccg gatacgttct gaaagagatc
     3601 aaagattgat atggagaatc actcgcacca ccataaacac gatgaggctc ttaaaatatt
     3661 aaagacagcg aggggccaga tagacggaat aatcaggatg atcgaggacg agaggtactg
     3721 tatagacata tccacgcaac tcctggctgt ggtctcgctt cttaaaaagg ctaatacctc
     3781 cgttgtcaac aggcacatcg aaacctgtgt aagagaggca gtgaaaaccg gcgaggtaga
     3841 tgagaagata aaagaactag aaacgcttat gaagtacata gagaagagcc tctaggctgg
     3901 tcctggaggt tcttactcat ctttcagaag ttcgtgcagc ttttcgagag cctctttgag
     3961 attattttcc ggaaggtccc agagattctc caccattctc ttcacgacct cgttcctgtc
     4021 ttctatagct ttcatataaa tggaaagcat atcccgtctc tctctcttca acatccccag
     4081 atttttcaga gtatctagcg agagcttttt ttcttcgtct ataaggtcgc ctatggtcag
     4141 gacgacatcg tccagtttcc actgcacatc gtccagcagc atcgccagtg ttctgcgtat
     4201 cgattccagt ctttcaactt tgcctgtcat attcaacctc cagcagcaat tcaagagata
     4261 ctgcgagtcc cttcaggtct attttagtga gcgagattcc ctgtcggggt gccttacgag
     4321 caggctgatc gccgcgcctg cgatgctgag tatggctgat accagataac tgatcccgta
     4381 cccgccggtg atgtctcgcg caatgcctcc gagaaggggg ccaagtatgc ctccgacacc
     4441 ccaggccgtt atcatcaccc cgtaattcac tcccaggttc ttcatcccgt aaaaatctgc
     4501 ggtgatcacc gggaatatgg ccagcattcc gccgaaggta aagccgacga gactcttgcc
     4561 tatcaacaga gcgaccgggc tggtaagagc agcgaacgag aaatagacga ccgcctgtat
     4621 gagaaacata gtcaaaagcg tgatcgttct tccgagataa tccgatatgg taccccacgt
     4681 aacccgtccg atgaagttga atatggcgta aacgacaacc agcaggaatc cgttactgat
     4741 cgaagcctgt tcgagcccga tcttcgacat ctggccgatt atgagcaggc ccgcgaaagt
     4801 cccgaagaag aacatggtcc agagaaggta gaactgaacc gtcttgacca tctcgcgaac
     4861 tttgtagtcc tttgccgtta tctgatgggc cctagccggt ctcctcttca gttcgattct
     4921 ctggaaaccg ggcggcggat ttgaaatggt gaaagagagc gaaaaaacga ccagaaagaa
     4981 gattataccg agagtgatta aagttgccgg aagccccaat aatcctataa gggaactggt
     5041 caagggggcg atataaacgg gtgcgagacc gaaaccgctt acaactatgc ccgatatcaa
     5101 gcctctgtgt tcggggtgaa accattttac ggcggcgggc gtgggtgaag cgtaaccgaa
     5161 acccatcgcc agaccgaaga ccacgccgaa gaataaagtg agccccgcaa ctgtgagatt
     5221 gagtccggag agaatgaatc ccagaccggc cagcactgaa gaaatcaaaa gcaccggttt
     5281 tggtccgagc ctgtcctgca attttcctcc cggaaccatc gtgagcgcga aaaccgcgga
     5341 agctagcata taggggatct gcgtctgtgt tgcgctccaa ccataattgt ctatcaaggc
     5401 tgcggagatg atgctccagg agtaaagcac tccaagggtt aagttaactc ccaacccact
     5461 gtaaactacg agccaggcct tttttagatc ggtcacgaca gatttgtcct cctaatgaaa
     5521 agtaatgcac accgggcaga tctgtttcat aaagtagcac agcttacccc atttttacaa
     5581 tgaccacaac ggtagtgttg aacagtcagg atcacttttt cttgctcaac aggcttttga
     5641 aagagacagg cacaaaatac tgaatgcaga cgatcttcca tattcccttt gatttaatca
     5701 ggagcgtgga accctggatt tcaactctcc tgaaagcggc gtttctggct aacttaactc
     5761 cgcgtttcca caacataaga cattcgccgg acttttcgat actgctctgc cagaggaaag
     5821 gttcgtaaag aactccgaaa gagt
//
LOCUS       DAOOLY010000152         5811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16513_length_5811_cov_4.721161, whole genome shotgun sequence.
ACCESSION   DAOOLY010000152 DAOOLY010000000
VERSION     DAOOLY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16513_length_5811_cov_4.721161"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..978
                     /locus_tag="PLD15_08110"
     CDS             367..978
                     /locus_tag="PLD15_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HPB63910.1"
                     /translation="MKLIYATTNISKIEYMRKMLDGLGIDILAPDRHIALPGIPEEGD
                     TPAENAYIKARAYYEVIGKPLFSCDSGLYIQGLDDNRQPGSRVRRVNDRELSDEEMIE
                     YYSKLVKELGGIARASYRNAICLIIDRERVVTYDGEDLAESFLLVDKPHAIRKPGFPL
                     DSLSVEISSGEYFVELYEKGKINSDGCDKGFRKFFTSLRLNSR"
     gene            1761..3329
                     /locus_tag="PLD15_08115"
     CDS             1761..3329
                     /locus_tag="PLD15_08115"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB63911.1"
                     /translation="MRRLLIAILFVLFIFHASLFACTDIMVGKLATTDGSVISSQTVN
                     GTYDSRIIIVPAADHEPGEMAPVYEWIVYGDRMPLVKLGEIPQVEHTYQYFNNAYPFG
                     NEHQLLIGETTQSGARETANSEFAIMTIEQLEVFALQRCKTAREAVQLMGDLAVEYGY
                     RESCNLGEGLSVADPNEVWFFEVYGVGPLWKPGEGPGAVWAAQRVPDDHVSINGNYSM
                     IGEIDLKTNRPPNVPEGDFLICDNYLDTAIEIGRYDPNSGEPFVWKYVYGNIKGKWSS
                     RTARFFTLLNPSGNWDYNKTDDYPFSFKPDNKLSVYDVIELFRDVATGTPNDAEAAEA
                     WYYKDSKGNPVKSNLASPQPGNDIKALLGIATVSNFASHSTSCFYINQCRSWLPDPIG
                     GVVWFGLSNSQKTVVVPLYCANTEVPVSWTIVNRSDKTVDRNAAYYAFATVNKLSNQR
                     YQVFMPQVIEVRDPIQQRLYKMQDSIEKVALELYNQDPALAIEFLTNYTSSVMLEVEK
                     AYWKLADTLVLQLQ"
     gene            complement(3629..5725)
                     /locus_tag="PLD15_08120"
     CDS             complement(3629..5725)
                     /locus_tag="PLD15_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB63912.1"
                     /translation="MSMKILVVDDSATDRVLIERILSEYDVLTACDGVDAMKQIDANN
                     EIGLIILDLNMPRMNGFEVLKVLKSEEKYEGIRTIILTNYDELDNEIKGLELGAVDYI
                     RKPIHLASLKTRVGIHLELIKIQRRLEQKLVEQRSTFDTIFNQAPIGISISYSSDSSD
                     AISDVDTQINSMFEQIAGRTKEEMIELGWANITHPEDLEKDLEYYRKLKSGEIESYAM
                     EKRFVRPDGSIVWVEMTVASLKLMGESEIAHIALLKDITRRKAIEENLKYSYEHDSWT
                     GLYNRNYMENLLESDSRRGFHEKRAVVSINLSAMQSLTTTYGFHYTQELNKKIADILG
                     QYCNGGRVLFNIYENRFAFYIKGYEDKNELTEFSEEIAKTLEPLLITERISGGIGIVE
                     IDEENGRDVDGLLKKLLITSERAIDIDNREIGICFYDAAIEEQMVREQEIKRALTEIA
                     ADESYSRLFLQYQPIIELKTDVIIGFEALSRLNIDKLGLIPPLEFIPIAEKTKLIVPI
                     GRRIMLQAFRFLKRLNDLGYSSIGVSINVSAIQILRDDFTSSLFRMIEDARVHPEKIC
                     IEITESVVASNYEEMNRILGKLSDSGIHIAIDDFGTGYSSLARERDLNVNCLKIDKSF
                     INRLMYLKSEEAITSDIISMAHKLGHCVVAEGVEYQKQREYLERWGCDKIQGYLISKP
                     LNEEAAIEFLKTYNHW"
BASE COUNT         1524 a         1377 c         1320 g         1590 t
ORIGIN      
        1 cgaacgacgg tgtaagagaa ctgaagaatg cccaggacgg aacaggctac aggcgtggcg
       61 cctctggcga aaagggaccg ggactcggca gaggattcgg aaaagcggca cagcagtaat
      121 tgctaaaacc cctacccatt caattcaagg gcccgaaagg gcccttgttc ttgtgataat
      181 aaaatcgaag acaacagacg tcaggatccc gtcaatcttc attttcgcct tgcctgtcat
      241 cccgtagtga tcctatacgg aatttcgttc tattgattag attaaggaaa acttgaaaac
      301 ttgtaatcca ggtcatgata acatcttttt gcggctccat acttgcaacg gaggtcaaac
      361 tactttatga aactgatata tgcgactacc aacataagca agatagagta tatgcgaaag
      421 atgctcgatg gtctggggat cgatatactg gcaccggaca ggcacatcgc gctccccggg
      481 atacctgagg aaggcgacac cccggccgaa aatgcgtaca taaaggctcg agcgtattac
      541 gaagtgatcg gaaagcctct cttttcctgc gactccggcc tctatataca gggtctagat
      601 gataatagac aacccggatc gagagtaagg agagtcaacg acagggaatt gagcgacgaa
      661 gaaatgattg agtattactc taagctcgtg aaggagctcg gaggcatagc gagggcgagc
      721 tacaggaacg cgatctgcct gattattgat cgtgagagag tcgtaactta cgacggggaa
      781 gacctggccg agagtttcct gctcgtcgat aagccccacg cgataagaaa acccggcttt
      841 cctctcgact cgctgtcggt ggaaatcagc agcggtgagt acttcgtcga gctctacgaa
      901 aagggaaaga tcaacagcga tggctgcgat aagggcttca ggaagttctt caccagctta
      961 agactcaact cacgataatc ccggcttacg caatttgact tagccgtggt cacccagtga
     1021 gatagggtga gaaaaatgaa ttcgcaactc gagaattaag tttctcagtc ggaatatgga
     1081 ttaaatgagc ttgtttccgg atcggtaaac gcaaaacaga agcatataat taacagtgac
     1141 attctctgtc gcctgaggac gatagtcttt gctttccata tcccgtggag gtgatcaaat
     1201 gatgataatc gccagcttaa ttccactcgt catcgcgggt cttgctattg ccggtatcgt
     1261 tgtaatagcc tactggatcg cgctgattat aaagtacttt tagaaaccat aatctaatta
     1321 gcgggtggag actgcctgtt gaaatcgaag cgattcccgc gtagttgttt cccatcaact
     1381 caaactgcca gcagttagga tttttcggat tacgtacaat atagaagaag tgatatgatg
     1441 caactgtaga atattagcat cgcgaacttg cttcgaatag ccctgagggt tgccaatacg
     1501 gttttacgag ttttctagcc gtacggtctt tcgatcatat tttgcttcca ggaaaatgaa
     1561 taccggtgat ctggctcgat aattctgagc ctttttgttg tgcaaaacac atgagactcc
     1621 ttactccttt gaagggaggc catgggagag gatgagcgag gagcacaaat agccgtatct
     1681 tctttgatta cttcattcga agccagacat tcttcacaaa acaaaatact gtttatgatt
     1741 ttcaaggagg ggaattgata atgagaagat tgttgatcgc cattcttttc gtgttgttta
     1801 tcttccacgc aagtctgttc gcctgtaccg atatcatggt cggaaagctt gcaaccaccg
     1861 acggttctgt catttcttca cagactgtga acggtacata cgattccaga atcatcatag
     1921 tgcccgcggc agaccacgaa cctggggaaa tggctcccgt gtacgagtgg attgtctacg
     1981 gtgacaggat gccgcttgtg aaactcggtg aaatcccgca agtcgagcac acgtaccagt
     2041 actttaacaa cgcatatcct tttggaaatg agcaccagct ccttataggg gaaaccactc
     2101 agagcggtgc aagggagacg gcaaatagcg agttcgcaat catgactatc gagcagctag
     2161 aagttttcgc tctccagaga tgcaagactg ctcgtgaagc ggtacagctc atgggcgacc
     2221 ttgccgttga atacggctac agagagtcgt gcaatttggg cgagggttta tcggttgccg
     2281 atccaaatga agtctggttt tttgaagtct atggagttgg acccctctgg aaaccgggcg
     2341 aaggtcccgg tgcagtatgg gcggcccaga gagtgcccga cgaccacgta agcattaatg
     2401 gaaactacag catgataggt gaaatcgacc ttaagactaa tcgcccgcca aatgttcctg
     2461 aaggcgactt cttgatctgc gacaattacc ttgatacagc gatcgaaatc ggcaggtatg
     2521 atccgaacag cggggagccg ttcgtttgga aatacgtgta cggaaacatc aaggggaaat
     2581 ggagttcaag aaccgcaaga ttcttcacgt tattgaaccc gtccggcaac tgggactaca
     2641 acaagaccga tgactatcct ttctctttca agccggacaa caagctatcg gtgtacgatg
     2701 tcatcgagct attcagagat gtcgcaactg gaaccccgaa tgacgcggaa gcggccgaag
     2761 catggtacta caaagacagc aaaggtaatc cggtgaagag caacctggcc tcccctcagc
     2821 ctggaaacga tatcaaggca cttctgggga tcgcgacggt cagtaatttc gcttcacatt
     2881 ccaccagctg tttctacata aaccagtgtc gtagctggtt acccgatccg ataggcggtg
     2941 ttgtgtggtt tgggctttcc aactcgcaga agacagtcgt ggttccgctt tattgtgcta
     3001 acaccgaagt cccggtgtcg tggacaatag taaatagaag cgacaagacc gttgatagaa
     3061 atgccgccta ctatgccttc gcgacggtga acaagctttc taaccagagg taccaggttt
     3121 tcatgcctca agtcatcgaa gtcagggatc cgatacaaca gaggctgtac aaaatgcagg
     3181 attcaattga aaaagtggct ctggagctat ataaccagga tccggctctt gcaatcgagt
     3241 tcctgacaaa ctacacctcc tctgtaatgc tggaggtcga aaaggcgtac tggaaactgg
     3301 cagacactct ggtacttcag cttcaataag ttcagtcact tagttccata tagaatatga
     3361 gctgctgatt taggtcagca gctctttata ttgcggggag gctttttatt gaaatatcta
     3421 tcaggatgtt tatctcggcg ttgatatcgc gatagttcat tgacccacac taagggcaga
     3481 accattctct gccggaaata ctcaaattct ccttaattga aatcgagagt cttcttggtg
     3541 tttgtttata aagaagcggt gttagattgc agactttcga agaacttaca attcctcttt
     3601 gcggcatcca tcaatcgatt cgaattcact accaatgatt atacgtcttt aggaattcga
     3661 tcgccgcttc ttcattcagt ggtttactga tcaagtaccc ctgtattttg tcgcaacccc
     3721 atctctccag atactctctt tgcttttggt actccacacc ttccgccaca acacagtgtc
     3781 ccagtttatg agccattgaa atgatgtcgc tggtgatggc ctcctcgctt ttgaggtaca
     3841 tcagtctatt tatgaaggat ttgtctatct taaggcagtt cacatttaga tccctctccc
     3901 tggcaagaga ggagtagccc gttccgaaat cgtctatggc tatatgaatc cctgaatcac
     3961 taagcttgcc tagaatacgg ttcatttcct catagttcga agccacgacc gattccgtga
     4021 tctcgatgca gatcttttca ggatgaacac gggcatcttc tatcatccgg aagaggctgc
     4081 ttgtgaaatc atctctcagt atctgaatgg cggagacatt gatcgaaaca cctattgaag
     4141 agtaaccgag atcgttcagc cttttcaaga agcgaaacgc ttgaagcatg atccttcttc
     4201 cgatcgggac aatgagcttc gtcttctcgg ctatcgggat gaattccaga ggaggtatca
     4261 ggccaagctt atctatgttc agtctggaca gagcctcaaa accaattatc acatcggttt
     4321 ttagttctat gatcggctgg tattgtagaa acagcctgct gtagctctca tcggctgcga
     4381 tttcggtgag cgcgcgtttt atttcctgct cgcgtaccat ctgttcttca atcgccgcgt
     4441 cgtagaaaca tatgcctatc tccctgttgt cgatatcgat agccctctcc gatgtgataa
     4501 gaagtttctt caggagtcca tcaacatctc gtccgttctc ttcatctatt tcaacgattc
     4561 ctattccgcc actaattctt tcagttataa gaagcggttc cagggttttt gcaatttcct
     4621 cggagaattc agtcagttca ttcttgtcct cgtagccttt tatatagaaa gcgaatcgat
     4681 tctcgtatat gttgaaaagc actcgcccgc cgttgcagta ttggccgagt atatcggcga
     4741 tcttcttatt cagttcctgc gtgtagtgaa atccatatgt agtcgtcaat gattgcatcg
     4801 cgctcaggtt gatgctcact actgcccttt tctcgtgaaa tccccttctc gaatcgctct
     4861 ccagaagatt ctccatgtag ttgcggttgt aaagtcccgt ccagctatcg tgttcatagc
     4921 tgtacttgag attttcctcg atcgcttttc ttcttgttat atccttaaga agagcgatat
     4981 gggcaatttc actttcaccc ataagtttga gggaagctac cgtcatttca acccatacga
     5041 tggaaccgtc aggcctgaca aatctctttt ccattgcata actttcgatt tctcctgatt
     5101 tcagcttcct ataatactct agatccttct ccagatcttc tggatgcgtg atatttgccc
     5161 agccaagctc gatcatctct tctttcgtcc tgccggcaat ttgttcgaac atcgagttga
     5221 tctgtgtatc tacatcacta attgcatctg aggagtcact gctgtaagaa atcgaaattc
     5281 caatcggtgc ctggttgaag atcgtatcaa acgtcgatct ttgctcgaca agtttctgct
     5341 ctagtcgccg ctgtattttg atgagctcca gatgaattcc gacccttgtc ttcagagaag
     5401 ccagatgaat gggttttctt atgtagtcga cagctccaag ctccaaccct ttgatttcat
     5461 tgtccagctc atcgtagttt gtcagtatta ttgtgcgaat cccctcgtat ttttcttccg
     5521 acttcaggac tttcaacact tcgaatccat tcattctggg catattcaga tccaaaatta
     5581 tcagacctat ctcattgttc gcgtctatct gcttcatggc atctacgcca tcgcaagcag
     5641 tcaatacatc gtactcgctc aaaatacgtt cgataagaac cctatctgta gccgaatcgt
     5701 cgacaaccag gattttcatt gacatagatc atccccatta aacaatagaa tcatcacaga
     5761 ctcataaaaa aagaggctta cagaactgcc cgccatacta cacgctcacg t
//
LOCUS       DAOOLY010000153         5770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16687_length_5770_cov_4.302362, whole genome shotgun sequence.
ACCESSION   DAOOLY010000153 DAOOLY010000000
VERSION     DAOOLY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16687_length_5770_cov_4.302362"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLD15_08125"
     CDS             <1..685
                     /locus_tag="PLD15_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPB63913.1"
                     /translation="EIANMHWLGLTTITYDDFDYIAQFNEDAAGVIVKADAPWNTVHE
                     LLADIKANPNKFFFSGSSAASIWDLARIGMFSEAGVPVDSVTWIPTTGAAPSIIELLG
                     GHVDVITCSIAEAASQLDSGQLKALAVMSDERLQRFPNVPTLKEQGINWSAGTWRGIS
                     VPKNTPTDVKAILEAEVLKIANSEAFKEFMDINGFGIKIRNGVEFYEFAKQQDSAWKS
                     VLELGGFIQ"
     gene            749..1246
                     /locus_tag="PLD15_08130"
     CDS             749..1246
                     /locus_tag="PLD15_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPB63914.1"
                     /translation="MNIKNIFFGISLLVLSGLIFFFGSSFPNYVVRGNKLPGPKFFPF
                     TMAVIIIILGAYYIIKSLIMIKLKRIPKDAALAPSDKITLDGIKNVIAIIAGIIVFVP
                     LINLLGFILGATIISTALMIILNVKVWRSLVYSVILVVAIFLIFGMVFKIPLPEGIVV
                     AMFQG"
     gene            1251..2741
                     /locus_tag="PLD15_08135"
     CDS             1251..2741
                     /locus_tag="PLD15_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPB63915.1"
                     /translation="MDIILSILSPDVIFPVLFAMTFGIFVGGLPGLTATMAVALIIPI
                     SYYMSPLAGLGMVLGVSFTSIFSGDIPATFLRIPGTPASGAATLDGFELAKKGKGSLA
                     LTLDLFCSAIGGLIGILLLILIAPPLANFALQFTNFEYFWLGLFGLSMSAVLSKGNMM
                     KGLTSAVLGLAISTIGIDITTGYPRFTFGNIELMDGIGFIPAMIGLFGISEVLKRVQQ
                     GAAKLQLPPITEKVKISPIEALKIIWKEKMTLIKAAFVGTFIGALPGAGADIAAWVAY
                     GVEKKTSKNSEDFGTGNIKGVIAPTSANNAALGGTWIPALVFGVPGDTITAIVLGAML
                     MYGLKPGPLIFTESRELVNGVFGIAVLANILMIPIGYLGIKAFAQVLKLKTSVVLTGV
                     VLFSMIGSYAIRNSYFDIYVMLAFGFIGYIMERLAVPLAPMILGIILGPMVEDNLRVG
                     LIKTGGSMNQFFTRPISLVLFLLILLVFLGGPTVGLLKKAFKSKKS"
     gene            3476..>5770
                     /locus_tag="PLD15_08140"
     CDS             3476..>5770
                     /locus_tag="PLD15_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63916.1"
                     /translation="MRRFVLFILFAFSLFFVITGCVVSNPKVERLVPPENAPEEVIFA
                     PLSDSGVQSSSLIVGADTVIGRIEYWVDDEEKLLYIDFYATENGWLLTETRVATALSL
                     AELPRNPETRRLDPDYYPNFNIHNPPVGISHFVLPLGELEPGTTTKLYFSAYAEAIRK
                     ENKYFSQIGITAPCVYYVITYTEPVIELRPILNVEVDGKIALSRVTEYDWQLTKEATP
                     EAIVDLERGDSATVEYTLTATRSVPEIKDTYTASGVATVTNYGSLEATKVGLKVTLLG
                     KKGTDWLEIDSKVLIEISSNTTIAASDLVGIGKPFTFTFDAGDYTEVKILAQAEDDWP
                     ESVSDEENHMVPVSPTGETKIDESASVIDMPVNLADFESRGFLVDHTGTWPWDESPWI
                     LDDVSGGQKIYSISIKNESADENSFGVTLTNIATLTESDTLEIREDNARVLVTTPEPF
                     IETPDLDMTLEATVSWTGVLEYDWTIEKSATPTSLTLAQGNSGTVNYSVAVTRVSGDA
                     TYTYTISGTATVLNTGNIALNVAGAVNLDGYAGKTVSIGPLIIAAGASHEVPFTFTVE
                     SATEIINFTVKGNAHSVDLPVNVDKSLGLVSPGTPESYTEIDEMADVIDKITGLPSGF
                     KATASVEPREWKDLTDDATFTYIVTVTNEEATSGGKLTNTATVTENDTDEEDSDDEEV
                     TVNVPGLNMVLEATVNWSKEIEYDWSIAKDATPTSLTLVQGSSGTVDYTVVVTKESKD
                     ATYTYTVSGKVIVQNTGTVGLTGVN"
BASE COUNT         1626 a         1241 c         1380 g         1523 t
ORIGIN      
        1 ggagatagct aacatgcact ggttaggcct tacaacgatt acttatgacg acttcgatta
       61 tattgcacag ttcaacgaag acgccgcagg tgtcatagta aaggccgatg ccccatggaa
      121 tactgtccat gaacttctgg cagatatcaa ggcaaatccg aacaagttct tcttctcagg
      181 ttcgagcgcg gcatccattt gggatctggc gaggatcgga atgttcagtg aggcaggagt
      241 tccagtggat tccgttacat ggataccgac gacaggggcg gcgccttcga taattgaact
      301 gcttggcggc cacgtcgatg ttataacctg ttcgatcgcc gaagctgctt cccagcttga
      361 cagcggccag ctaaaggctc tggctgttat gtcagatgaa aggctccaga ggttccccaa
      421 cgttcccaca cttaaagaac agggaatcaa ctggtcggct ggtacgtgga gaggcatctc
      481 cgtaccgaag aatactccga ccgatgtcaa agcgatcctc gaggctgaag tactcaagat
      541 tgcaaattcc gaggctttca aagaattcat ggatatcaac ggctttggta tcaagataag
      601 aaatggagtg gaattctacg aatttgccaa gcaacaggac agcgcctgga aatctgtgct
      661 cgaactcgga ggattcattc agtaattgcc gttaacgggt ggcccggagg gctacccgtt
      721 tcttcaatta agtaatcggg gtgattggat gaacataaaa aacattttct ttgggatttc
      781 acttctggta ctttccggtc ttattttctt tttcggctcg tccttcccga actacgtggt
      841 gcggggaaac aagttaccag gaccgaagtt tttccctttc acgatggctg taatcataat
      901 catactgggc gcatactaca tcatcaagag cctgataatg ataaagctaa aaaggattcc
      961 taaggacgct gctcttgcac cttctgacaa aatcacttta gacggtataa aaaatgtaat
     1021 agcgattatc gcgggaataa ttgtcttcgt acctttgata aacttgctgg gcttcattct
     1081 tggagccacg atcatatcta cagctttgat gataattcta aacgtcaagg tctggcgttc
     1141 tttagtatat tcggtgattc tggtggtcgc tattttcttg atattcggca tggttttcaa
     1201 gattccgctt cccgagggaa tcgttgtggc catgttccag gggtgatcgt atggatatta
     1261 ttctctcgat cttaagcccg gatgtgatat tccccgtact tttcgccatg actttcggca
     1321 ttttcgtcgg tggattgccc ggtctaacgg ctaccatggc agttgctttg ataataccta
     1381 tcagttatta catgagccct ctagcgggtc tgggaatggt actgggggtt tctttcacat
     1441 cgatcttctc cggcgatatc cccgcgactt ttctcaggat tccgggaact ccagcctctg
     1501 gagcggccac cttagatggt ttcgaactgg cgaagaaagg gaagggctcg ctggcgctga
     1561 cgctggatct cttctgctcg gcgatcggcg ggttaatcgg aatactttta ctcatattaa
     1621 ttgcaccgcc actggcaaat tttgcccttc agttcaccaa tttcgaatac ttctggctcg
     1681 ggctttttgg cttgagtatg agcgccgttt tgagcaaggg aaacatgatg aaaggactga
     1741 catcggccgt cctgggactt gcgatatcta caattggtat cgatataacc acgggatacc
     1801 cgcgcttcac ttttggaaat atagagctta tggatggcat aggtttcatc cccgcaatga
     1861 tcgggctctt tggaatctcc gaagttctca agagagttca gcagggagcc gccaaactcc
     1921 agcttcctcc tataaccgag aaggttaaaa tctcgccgat agaagccctg aagataatat
     1981 ggaaagaaaa gatgacccta atcaaggcgg cctttgtggg gactttcata ggcgctcttc
     2041 caggtgccgg tgcagacatt gcagcctggg ttgcttacgg tgtagagaag aaaacctcca
     2101 aaaacagcga agacttcggt acaggcaaca taaaaggggt tatcgccccc acaagcgcca
     2161 acaacgccgc gcttggcggt acctggattc ctgcactggt cttcggtgtt ccgggtgata
     2221 ccataaccgc catcgtactc ggcgccatgc tcatgtacgg attgaaacct ggaccgttga
     2281 tattcaccga atcgagagaa ctcgtgaatg gagtcttcgg aatcgcagtt ctggcaaaca
     2341 tactcatgat acctataggt tatctaggaa taaaggcttt cgcgcaagtt ttaaaactca
     2401 aaaccagtgt agttcttacg ggagtagttc tcttctcaat gatagggtct tatgctatca
     2461 ggaacagcta tttcgatatc tacgtgatgc tcgctttcgg attcataggc tacatcatgg
     2521 aaaggcttgc agtgcctctg gctcccatga tactggggat catattgggt cctatggtcg
     2581 aagataatct cagggtcggg ctaataaaga ccggtggaag catgaatcag ttcttcacca
     2641 ggcctatttc tctggtgctg ttcctgttga tcttgctggt cttcctcggt ggaccgacgg
     2701 tcgggttact caaaaaagcc tttaaaagta agaagtcata atcctctaga attgcaaata
     2761 tgaagagaaa gaaccgattg gattcaatcg gttctttttt tttatgtatc aagtccctgg
     2821 cggaaaacgg gttaaagaac aactcataag aagaatcgag taaggaaaag gtattcgata
     2881 taaatcaaca tacaaaaaca ggggtgttcc atactacagg gtttcttgaa tgatcttgta
     2941 ccagactcac tttgaaatgc cttctttttt ctttttttga agtatatacc gtgcaatttt
     3001 caaatgtgaa atagaaaaaa acggtataga tacagatatt tgttttaatg acaatcactt
     3061 ttccgattat caatttggcc agttagagct atgaacagaa aaggcgctaa agttctttgt
     3121 gtgtatatat tctatcgtat gattatatca tatatagtta atcgcacgaa taacacaaat
     3181 aatgaatggg tatttttaat agtataaagt catgtcgaag ccgctgatag attgtatata
     3241 gctattttag ctcttagtat tattagcaac tgatactata acaattctca agaatctata
     3301 gcgactcaat tttttactct tttgacagat aatttgtaac ataataagtc aatcgaatgt
     3361 attggatttt tttgaattta tcttataata attctcgaat aggaatagtt cagttttctt
     3421 tgaaacactt tcattagttt gcgtttttgg ggtggaaatt taggaggcta taaatatgag
     3481 aagatttgta ctcttcattc tgtttgcttt ttcactcttc ttcgttataa caggctgtgt
     3541 ggtttccaat ccaaaagtag agaggctagt tccacccgaa aacgcacctg aggaggttat
     3601 ttttgcccct ctaagcgata gcggtgtaca gtcatcttcg ctcattgttg gggccgatac
     3661 ggtgattggc cggatagaat actgggtaga cgatgaagag aagcttctgt acatagattt
     3721 ctatgcaacg gagaatggct ggttgctgac ggaaacgaga gtagctactg cgctttctct
     3781 tgcagaactt ccaagaaacc cagaaaccag gcggttagac ccagattact atccaaattt
     3841 caacattcac aacccaccgg taggcatatc gcactttgta ttgcctctgg gtgaactgga
     3901 gcctggaaca acaacaaaac tttacttctc ggcctatgcc gaagcgataa gaaaagagaa
     3961 caaatacttc tcgcagatag gaatcactgc tccgtgtgtt tactatgtta taacctacac
     4021 tgaaccggtg atcgaactta gaccgatact caacgtagag gtagatggca aaatcgctct
     4081 ctcaagagta accgaatatg attggcaact gacgaaagaa gccacccccg aggcaatagt
     4141 cgaccttgag cgcggcgata gcgccacggt cgaatatacg cttacagcta ccagaagcgt
     4201 accggaaatc aaagatactt atactgcatc cggagtggct acagtcacca attacggaag
     4261 tctggaagca accaaagtcg gtctcaaagt caccttactg gggaaaaaag gtacggactg
     4321 gcttgagata gattcgaaag tattgataga aatcagctcc aacacaacga tagcggcgag
     4381 cgatctggtg ggtataggaa aaccattcac tttcaccttc gacgcaggcg attacacaga
     4441 ggtgaagatt ctcgcgcaag ccgaagacga ctggcctgaa tcagtgagtg atgaagaaaa
     4501 ccatatggtg cctgtctctc ctaccgggga gacaaaaata gacgaaagcg cttcggtgat
     4561 agacatgcca gtcaatctgg cggattttga aagtcgaggg ttccttgtgg accacaccgg
     4621 tacatggcca tgggacgaat cgccatggat cctggacgac gtttccggtg gtcagaagat
     4681 ctattcgata tcgattaaaa acgaaagtgc tgacgagaac tctttcggtg tgacgctcac
     4741 gaacatagca acgctcacag aaagtgacac cctggagata cgtgaggaca acgccagagt
     4801 attggttacg actcccgagc cattcataga gaccccggat ctagatatga ctctggaagc
     4861 aactgtaagc tggacaggag tgctcgaata cgactggaca atagaaaaga gtgcaacccc
     4921 gacttctttg acactggcac agggaaactc tggaaccgta aactattcgg tggctgttac
     4981 cagagtgagc ggggatgcga catacacata tacaatatcc gggacggcta ctgttttgaa
     5041 cacgggaaac atagccttga acgtagcagg tgccgtgaat ctcgatgggt acgcgggaaa
     5101 gacagtctct attggtcctt tgataattgc tgcaggggcg agtcacgaag ttccattcac
     5161 cttcacggta gaaagcgcaa ctgaaataat caacttcact gtgaaaggaa atgctcattc
     5221 ggtagatttg ccggtcaatg tagataaatc tctagggctt gtttcacccg gtacacccga
     5281 gagctataca gagatagatg aaatggcgga tgtcatagat aaaatcacag ggttaccttc
     5341 aggattcaaa gcaactgcaa gtgttgaacc gagagaatgg aaggacctta cagacgacgc
     5401 aacattcacg tacattgtaa ccgtgactaa cgaagaagcc acttccggtg gcaaattgac
     5461 caacacggca acggttacag agaacgatac ggatgaagaa gactcggacg atgaagaagt
     5521 gacggtaaat gtacccggac tgaacatggt actggaagcg actgtgaact ggtcaaagga
     5581 aattgaatac gattggtcta tcgcaaaaga tgccactcca acttcgttga cccttgtaca
     5641 gggaagttca ggtactgttg attacactgt agtagttaca aaagagagta aagatgctac
     5701 atacacctac actgtgagcg gtaaagtaat cgttcagaac acaggaacag ttggactaac
     5761 gggtgttaac
//
LOCUS       DAOOLY010000154         5727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16843_length_5727_cov_3.597849, whole genome shotgun sequence.
ACCESSION   DAOOLY010000154 DAOOLY010000000
VERSION     DAOOLY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16843_length_5727_cov_3.597849"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLD15_08145"
     CDS             complement(<1..263)
                     /locus_tag="PLD15_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate
                     carboxylmethyltransferase"
                     /protein_id="HPB63917.1"
                     /translation="MVNLARTLAKMGYLNDERLFEALTMIDRRHFVEKDLVSAAYSDR
                     VCISFYERGKILSTSTQPSLLVSMIESLCLENHYRVLEIGTGTG"
     gene            complement(310..1014)
                     /locus_tag="PLD15_08150"
     CDS             complement(310..1014)
                     /locus_tag="PLD15_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB63918.1"
                     /translation="MTGTLAGFINVVGGGGSILTLPVLMWIGLPSSVANATNRIAILS
                     ESIAGAVGFSSKGIRPGRTVIPAIIFASTGSILGSLFVLSVQTERLNRVIAILLLLAA
                     FITLFGPPKGKVGKGSLLSTAVIFLAVGFYGGFIQAGVGFLLMAAITFVFGFNLVITN
                     ATKIMTVLVYTSFSVIVFASRGMIDWIPGLVLSLGNMTGAFLAARLSVRKGVKFTRWI
                     LVVVVLINAIKYLLFD"
     gene            complement(1090..1545)
                     /locus_tag="PLD15_08155"
     CDS             complement(1090..1545)
                     /locus_tag="PLD15_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB63919.1"
                     /translation="MIEEEVLSSKDDLLKLDRQLCFALYSGARGIIRMYRPILEKLDI
                     TYPQYLVLLVLWEYGQMNVRDMGEKLFLDSGTLTPLLKRMERQGIVLRERSIKDERTV
                     LISLTDKGRMLKEEALSIPLQLANNLDIDRKEYETLMVALKRLIAKMNL"
     gene            complement(1542..2018)
                     /locus_tag="PLD15_08160"
     CDS             complement(1542..2018)
                     /locus_tag="PLD15_08160"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPB63920.1"
                     /translation="MSIYDFDMVSIEGWNRSISEYRDKVLLIVNTASKCGFTPQYEGL
                     QKLYNDYKDRGFVVLGFPANDFLRQEPGTNEEIKEFCSVSYSITFPMFEKIHVKGKEI
                     HPFYEYLTAGGGKEEFAGKISWNFAKFLIGRKGEIIARFDPKDDPEKIRPYIEEAL"
     gene            2601..5084
                     /locus_tag="PLD15_08165"
     CDS             2601..5084
                     /locus_tag="PLD15_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HPB63921.1"
                     /translation="MKRGLVLLVLLCLIVVTGFAKTYVFEAIPETGLAKTILALEKLP
                     VFGTVFMIGAHPDDEDNGLIAYLAKGLHLETYYLVSNWGQGGQNEIGPELYEALGVLR
                     SQELASARAIDGGKQLCFNAYDFGFSKTAEETFNNPDWDKETIIGNLVELIRTYKPDI
                     VLSRHAPTGGHGHHQACGVLVPEAVAAAADPNMYPEQLEAGLQVWQAKKLYQAGSGGV
                     RVNTGEYDYVLGYSYNQIGRIARGSHQCQGMAGSLGTPGDAFSNYKLLSSTVGMEETG
                     MFDNLDFSLKAISNGIAGDPEVISAIDGMLLELNGITSSILAGFNAMDPTGIGDEVLE
                     GLSLVREIIAFAALSNLSDDNKELLVERLVMKEADFLAAAREVFSTKFLVSGNDSAIV
                     PGQSFEVTATFWNLGKEVIDKAELALVLPEGWTVDKESEAFEAINFNTNGQAKFTVTV
                     GADAAYTDAFDENPISAVAVWEVMDTSVWTESVADIRVVPKVSVSLDPERLMTTASSS
                     AITRTINVKVQNNSKEAVSGTVDLELPAGWEIVTKDTSFSLGKENESKSIQVEFKIPA
                     NAPLGESEIVAEAIVDGEVFNKGFQVIAYPHIETKNYYKSSVARVAVIDVEVASGLKV
                     GYVDGGLDINTVYLRQMGVDVTELSPADLASGDLSKYDTIVIGMRAATTRPDIEANSA
                     RLNEYVFNGGNLLVQYHKTREYLGSWAPFDFTISSNRVTVEEAEIKILVPDHPIFNWP
                     NVIGDSDWEGWKQERGLYFPSKWAPEFTPLIDTDDPGEDIPAGSLLIANYGKGTYIYT
                     ALVWYRQLDYLVPGGYRVFANMLSLPKVK"
     gene            5324..>5727
                     /locus_tag="PLD15_08170"
     CDS             5324..>5727
                     /locus_tag="PLD15_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPB63922.1"
                     /translation="MRIVRCEGLISRADIARKSGLSKPVVSVVVNNFISQGSIVEAKE
                     GESSRKGGKKPILLSFVPDYKYIVGVDVGGNKLISILSDLDGKIIEKAKFSTKSISDE
                     KAFFELVEKSVLAVMKVPVSKVMGIGIGVPGTV"
BASE COUNT         1571 a         1383 c         1320 g         1453 t
ORIGIN      
        1 ccagtccctg tgcctatctc gagaactcga taatgatttt caaggcataa actttcgatc
       61 atcgaaacaa gaagcgaagg ctgtgtcgat gtcgatagaa tctttcctct ttcatagaag
      121 cttatgcaga ctctatcgct gtaggctgcg ctcacaagat ccttttcaac gaagtgtctt
      181 cgatcgatca tagtcagagc ttcaaagagc ctttcgtcgt taagatatcc cattttggcg
      241 agagttctcg ccagatttac catagacaca gaaatccctc catgcaacca tcaccagaag
      301 agctcatcgc tagtcaaaaa ggaggtattt gatggcgttt ataaggacaa caacgacaag
      361 tatccacctg gtgaatttga caccctttct caccgacagc ctggccgcca gaaaagctcc
      421 agtcatgtta cctagagaaa gcaccaaacc gggaatccaa tcgatcattc ccctggaagc
      481 gaaaacgatc accgaaaatg aagtatatac cagtaccgtc atgatttttg ttgcgttcgt
      541 tataaccaga ttgaaaccga acacaaacgt tatcgccgcc atcaacaaga aacctactcc
      601 ggcctgaatg aaccccccgt agaaaccaac cgcgagaaaa atcaccgccg ttgaaagcag
      661 agagcctttg ccaacctttc ctttcggagg tccgaagagt gttatgaacg ccgccaatag
      721 aagcaaaatt gcgattactc tgttcagcct ctccgtttgc actgatagaa cgaaaagaga
      781 acccagtatt gaacctgtac tggcaaatat tatcgccggt attaccgtcc taccgggtct
      841 tatccccttt gaagaaaagc caaccgctcc ggcgatactc tccgaaagga tcgctatacg
      901 gttggttgca ttggccaccg acgaaggcaa cccgatccac atcaaaaccg gaagggttag
      961 gatcgacccg cccccgccga ccacatttat aaaaccggcc agcgttccag tcaaataaac
     1021 tatcaggaac tgagaccatg acagaatctc tccagatgcc gacaagatta ttgctctctt
     1081 ttttaaccgc taaagattca tctttgcgat cagtctcttc agcgcgacca taagggtttc
     1141 gtattctttc cgatcgatat ccagattgtt cgccagctga agtggaatgc tcagggcctc
     1201 ttccttcaac attcttccct tatccgtcag actgatcaga acggttctct catcctttat
     1261 agatctctct ctcaagacta tgccctgtct ttccattctc ttcaacagag gagttaatgt
     1321 ccccgaatcc agaaaaagct tttctcccat atcccggaca ttcatctggc catattccca
     1381 gaggaccagt aataccaggt actgcggata ggttatgtcg agtttttcaa gaatgggtct
     1441 gtacatcctt atgatgcctc tggcaccgga gtaaagagca aaacacagtt gcctgtcaag
     1501 ttttagcaga tcatctttac tggaaaggac ttcctcttca atcataatgc ttcctcaata
     1561 taaggtctta tcttctcggg atcatccttt ggatcgaaac gggcgattat ctctcccttt
     1621 ctaccaataa ggaacttcgc aaaattccag ctgatctttc ccgcgaactc ctctttgcct
     1681 ccccctgctg ttagatattc gtaaaaagga tgtatctcct tacctttgac atggatcttc
     1741 tcgaacatgg ggaaggttat actgtatgaa acactgcaaa actcctttat ttcctcattg
     1801 gtgcccggtt cctgtcttaa aaaatcgttg gccggaaaac cgagaacaac aaatcctctg
     1861 tctttgtagt cattgtaaag cttctgaaga ccttcgtact gcggagtgaa gccgcattta
     1921 ctggccgtgt tgactatcag gagaacttta tccctgtatt cggagataga cctgttccat
     1981 ccctctatag agaccatgtc gaaatcgtat atactcattt ctgcacctcc atataattgt
     2041 gtacaatatt tttgctatca ataatatagc acgtattttt ggaaattgca aataccaaac
     2101 tttcaactaa cacgctttga attaagagcc gtctgagacc accgagaaga tgatcgcatt
     2161 acccgtgacg atcgattgtt gaaaaaaatt gccgagaaca caataaaaac gagaagcttc
     2221 tttatgatgt ccttcgcttt agagaagcct tatggactgc atcagccagt tgtttccttc
     2281 cttgattttg aaccctgtct ttttctcgca cttgaaatgc gtgagtcaag acactagaaa
     2341 aacattcttg attttcggtc agcatttgcc ggtaacagta gattacggtg taccgaatca
     2401 attaagaccg ttcttcttat caaatcactg ctgaaggctt tgttctttta tctagacttc
     2461 agtcatcggt atttattata cgggtgggta ttaaggtata atatagtatg ttagtcatgt
     2521 ttactaacgt gttatccttt ttccggtcta gtgtttttga aaaagtgttc gtcgccagaa
     2581 aaaacaaggg aggtatggaa atgaagagag gtctggttct acttgtgctt ttgtgcctga
     2641 ttgttgtcac cggtttcgcc aagacatacg tgttcgaagc gataccggaa accggtctcg
     2701 caaaaacgat acttgcactt gaaaaacttc cggtatttgg aactgtattt atgatcggtg
     2761 cccaccccga tgacgaagac aacgggttaa ttgcttatct ggcaaagggt cttcatctgg
     2821 aaacatacta tctggtttcc aactgggggc agggtggcca gaacgagatt ggaccggagc
     2881 tttacgaagc tcttggcgtt ttgagatcac aggaactggc atctgccagg gcaatcgatg
     2941 ggggaaagca actctgcttc aatgcttacg acttcggttt ctctaagacg gccgaagaga
     3001 ccttcaacaa tccagactgg gacaaagaga cgataatcgg taatctggtt gaactcataa
     3061 gaacctacaa acccgatatc gttctctcca gacacgcccc aacaggcggt cacggccatc
     3121 accaggcctg tggcgtgttg gttccggaag ctgtagcggc tgccgcagac cccaacatgt
     3181 atcccgaaca gctcgaagcc ggactgcagg tgtggcaggc caagaaactt taccaggccg
     3241 gttccggcgg tgttagagtc aacaccggcg aatacgacta tgtgctgggc tactcctaca
     3301 accagatagg acgtatcgct cgcggtagcc accagtgcca gggaatggcc ggaagtctcg
     3361 gaacgccggg cgatgccttc tccaactaca agctccttag cagcactgtg ggtatggaag
     3421 agacaggcat gtttgacaac ctggatttct ctctcaaggc catctccaac ggcatagcgg
     3481 gagaccctga agttatttcg gcgatagacg gcatgctcct cgaattgaac ggcatcacca
     3541 gttcaattct cgccggattc aacgcgatgg atcctacggg catcggcgat gaagtcctcg
     3601 aaggactgtc gctggttaga gaaataatcg cattcgcggc cctatcgaat ctctccgatg
     3661 acaacaaaga acttctggtc gaaagattgg ttatgaaaga ggcagacttc ctcgcagcgg
     3721 ctcgcgaagt cttctcgaca aagtttttgg taagcggcaa cgactcagcc atagtgccgg
     3781 ggcagagttt cgaagtgacc gcgactttct ggaacctcgg caaagaagtc atagacaaag
     3841 ccgagctggc tctggttctt cccgaaggct ggaccgtcga taaggaaagc gaggctttcg
     3901 aagctatcaa tttcaacacc aacggacagg ccaagttcac cgttacggtc ggtgcagatg
     3961 cggcctatac ggacgctttc gacgaaaacc cgatcagcgc tgtggcggtg tgggaagtta
     4021 tggatacatc agtctggacg gaatcggtag ccgatatcag ggtagttccg aaggtctccg
     4081 tctctctcga tcctgaaaga ttgatgacta cggcatcttc ttcggccata accagaacga
     4141 tcaacgtgaa ggttcagaat aactccaaag aagctgtttc tggaacagtg gatctagaac
     4201 tccccgcagg ttgggaaatt gtaaccaagg atacctcttt ctctctgggc aaagaaaacg
     4261 agtccaagtc gattcaggtc gagttcaaga tcccggccaa cgcccctctt ggcgaatcgg
     4321 aaatcgtcgc agaagcgata gtcgatggag aggttttcaa caagggcttc caggtcattg
     4381 catatcccca catcgaaacc aagaactact acaagtcatc cgtcgcgaga gtggcagtta
     4441 tcgatgttga ggtagcctcg ggtctaaagg ttggctatgt ggatggcgga ctggatatca
     4501 atactgtgta tctgagacaa atgggagtgg atgtcaccga attgagccct gccgaccttg
     4561 cttcgggaga tctcagtaaa tacgacacta tcgtgatagg catgagagca gctacaacca
     4621 ggcccgatat tgaggcaaat tcggcaagac tgaacgagta tgtgttcaat ggcggtaatc
     4681 tcctcgttca gtatcacaag acacgcgaat atctcggcag ctgggctccc ttcgatttca
     4741 ccattagcag caacagagtc accgtagagg aagccgaaat aaagatactc gtccccgatc
     4801 acccgatttt caactggccg aatgtgatcg gagattcaga ctgggaaggc tggaagcagg
     4861 aaagaggtct ctacttcccg agcaaatggg caccggaatt cacgcctctg atcgatacgg
     4921 acgaccctgg cgaagatata ccggccgggt ccttactgat agcgaactac ggaaagggta
     4981 cctacatcta cacagcactc gtctggtaca gacagctcga ttacctcgta ccgggtggct
     5041 acagagtctt tgcaaatatg ctgagtttac caaaggtaaa gtgatataat cgtcgaaata
     5101 ccggggacct ttggggtccc cggttcttta aaagagcgat acttaagaaa acaacgataa
     5161 tcgatcctaa ttgcttctta tgtccttgta ccaggctcaa ttctccagaa ttccagttcc
     5221 ttgagaagac gctcatgatg tataatgtta gtaagttagg ctacttaaca aaataaaggt
     5281 gattccagtg agtcgatgct tacgtgaaac tgaagaaaaa gtaatgcgaa ttgtgagatg
     5341 tgaaggactg atctccaggg cagatatagc cagaaagagc ggtttgagca agcctgtggt
     5401 ttcggtcgta gtgaacaact tcatctctca aggttcaata gttgaagcaa aagaaggcga
     5461 gagttcacgt aagggtggaa aaaaacctat ccttctgtct ttcgttcccg actataagta
     5521 catagtcggc gtcgatgttg gcggtaacaa gctgatctct atactatcag accttgatgg
     5581 aaaaataatc gaaaaagcta agttctccac gaagtctatt tccgatgaaa aggccttctt
     5641 cgaacttgta gagaagtcag tgctagcggt gatgaaagtt ccggtctcaa aagttatggg
     5701 gatcgggatc ggtgttcccg gaactgt
//
LOCUS       DAOOLY010000155         5726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16848_length_5726_cov_4.198025, whole genome shotgun sequence.
ACCESSION   DAOOLY010000155 DAOOLY010000000
VERSION     DAOOLY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_5726_cov_4.198025"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLD15_08175"
     CDS             <1..496
                     /locus_tag="PLD15_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492612.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPB63923.1"
                     /translation="IIAGGDSMRNYIDWNNKIYEGGEIILRGEVPILGICLGHQIVSR
                     VYGSVMYYSEERRWHNVKTLLDDPILEGLSDTLLVWENHAYAVAKLPDGFRLMASGSV
                     TPIQMIKHESKTIYGVQFHPETPGEFLSTPQGWKLLSNFAKISKAETVPVPLPPNGRK
                     VFPY"
     gene            complement(524..1138)
                     /gene="plsY"
                     /locus_tag="PLD15_08180"
     CDS             complement(524..1138)
                     /gene="plsY"
                     /locus_tag="PLD15_08180"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPB63924.1"
                     /translation="MRYLIFAAIGYFMGSIPFSYLVPLLFGVNITKVGSGNVGGTNVL
                     RNMGGWLGAATMVMDGVKAFVPIIIARSVFGISMTEGMMIGFFAAVGHSYSIFLKFRG
                     GKAVACTVGTLSALIPWYVAVFFGIWLPIVLITQFVSLGSVLALLLLSIILFFTQGMG
                     VGLWMLAFFALALFRHRKNVRALIEGKERKTDLIAAFKKKRGRQ"
     gene            complement(1135..2457)
                     /gene="der"
                     /locus_tag="PLD15_08185"
     CDS             complement(1135..2457)
                     /gene="der"
                     /locus_tag="PLD15_08185"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPB63925.1"
                     /translation="MATVLIIGRPNVGKSTLFNRLVGGRRAIVDDIPGVTRDFTVGRV
                     NWQQKTFQLVDTCGLFENPENIVEEKMKEVTLELLGEGDLLIFLVDGRQGVTSADIEL
                     SNYLRKQKKEIVFVANKVESEGAFYTAANDIYALGFGDPVKISAAHGLNIDELLDEIF
                     ERLEGIGHSVHMEEEEQSSLKVAIVGRPNAGKSSIFNWIVGSPRSLVTEVAGTTRDTV
                     DETLAIDGKAMTFIDTAGIRKKSKVKVMNVEYYSVMRAIDAIERSDVIVMVIDSTEGV
                     GNQDQRIAGLAEKNGKATILVFNKSDLIDERRRKALIRTVKQDLYFIDYSPVIFTSAI
                     SGKGMDLLIDSIKLVSEKIDLRIPTPLLNTLLSRYSETTPPPGKGKKRGKIYYGSHIS
                     GRPPLIMLNVNDPDLFTETYLRGIRNTIRQNFDRYEGTPVFLKLRRKR"
     gene            complement(2457..4172)
                     /locus_tag="PLD15_08190"
     CDS             complement(2457..4172)
                     /locus_tag="PLD15_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPB63926.1"
                     /translation="MEEKLERQDENLSMEEIFEQMDVSSARKGSRKEAEVFSIQPDGL
                     MVIMEGKLDGFVPLDQLVNDLNEYSVGDKIEVMVIKTNEEEGRSTVSEKRVHARQALS
                     RVEKAFREGTPIEGRIVSETNAGYNIRLLRTVPAFLPGSESGIRKGENAPEGNLKFKV
                     LRFKRQRNGINVVVSLRAFQDEAIKEYFEKLEVDQITEGTVESIKNFGAFVRLSDNVT
                     ALIPASEMSWDPGARISDLLKIGSTVKAKIIGIDESEKKISLSLKQMSEDPWATLEER
                     YPVDSTAAGIVKNITPFGFFVSLEPGVEGLVHISEVFWGNIKKDLKSVVEVGDEVKVN
                     IKEINIEKRTLSLSYREAMGDPWENIGQKYNEGDIVKAKTAKVLPTGVILELEEYVSG
                     FVPVSEVSWNFVDRIEDVVKEGDETEVKILSIDSVNRRMRLSIKQATADPWERVSREL
                     SVGDYISGTVTRLTKSGAIVMVDSYGVEAFLPVSQVSIERVENIEDAVSIGNHNQYKI
                     IRLVYDPDNDTRNMVVSIRQLIKDKEAAETEEVMKDLNGNGMTISSNLGEMIKNQLKE
                     EGETL"
     gene            complement(4144..>5726)
                     /gene="cmk"
                     /locus_tag="PLD15_08195"
     CDS             complement(4144..>5726)
                     /gene="cmk"
                     /locus_tag="PLD15_08195"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPB63927.1"
                     /translation="LSETTGPTETTVERFGRSGVRIAIDGPAGSGKSTVAKLVARDLN
                     FVYIDTGAMYRALALKAHSLKIEFSDESPMVELMNHTYFSLSENGITLDGRFLGEEIR
                     TREVSMLSSNIARYPYVRAYLTEQQRSLSRQGNVVMEGRDIGTIVLPDAELKIFLTAS
                     IEERARRRYAELIASLKEVDYESVLREMKARDLNDSSRELAPLKPAKDAIRLDTTGLS
                     ISEVVEKVAAMARKRQRVLLSKSVGFCYGVDRAVNEAIKILKSGKSVFATGEIVHNEA
                     VMKNLKDLGLEIIEDISSYDRVDGIAIIRAHGIDPGSEKRLREVFTGVIDLTCPIVYN
                     VFSLAVDLQARGSFVVVFGKRNHPEVTALSGRLERYLIVEPGEDYSSVVDRLKAIERI
                     AVVSQTTMSTDDFKDFSSFLEEELGDRVEIHNTICRITVERESEARRLACEADTVIVV
                     GGKNSSNTKKLLDIVGRLGKRAIHVQTTGDLPQGELGRTGLISGTSTPYDQIQKILDY
                     IDNEREVTDNGRETGTAR"
BASE COUNT         1241 a         1575 c         1292 g         1618 t
ORIGIN      
        1 aatcatagcc ggcggagatt ccatgaggaa ctacatagac tggaacaaca aaatatacga
       61 aggtggcgag ataatccttc gcggtgaagt cccgatcctg ggcatctgcc tgggccacca
      121 gatagtgtca cgggtgtacg gatcggttat gtactacagc gaagagcggc gctggcacaa
      181 cgttaaaact ctgttagacg atccgattct cgaggggttg tccgatactc tgttagtctg
      241 ggagaaccac gcgtacgctg tggctaaact tcccgacgga ttcagattga tggccagcgg
      301 cagcgtcacc cctatacaga tgattaaaca cgaaagtaaa acgatctacg gagtgcagtt
      361 tcatccggag actcccggag agtttctgtc aacccctcaa ggctggaagc tcctttcgaa
      421 ttttgcgaag ataagcaaag ccgagaccgt tccggtacca cttcccccaa acggtagaaa
      481 ggtctttccg tactgaaaga taacaacggg gtgggcgaag atattactgt ctgccccttt
      541 ttttcttgaa cgcggcgatc aggtcggtct ttctctcttt accttcgatc agtgccctga
      601 cgttctttct atgcctgaag agcgcgagtg cgaaaaaggc gagcatccag agacctacac
      661 ccattccctg ggtgaagaaa aggattatcg agagcaggag caaggccagg acggagccga
      721 gtgaaacgaa ctgcgttatc aggactatcg gcaaccagat cccgaaaaaa acggcaacat
      781 accaggggat cagagcagag agtgttccga cggtgcaggc gacggccttg ccgcccctga
      841 acttgaggaa gatcgaataa ctgtgaccga cggccgcaaa gaatcctatc atcatgcctt
      901 cggtcataga tatgccgaaa acactcctgg ctattattat cggcacgaag gcttttacac
      961 cgtccatgac catggtggcc gctcctagcc agcctcccat gttgcgcagg acattagttc
     1021 ctcctacgtt gccgctgcca actttcgtta tattcacgcc aaagagcagg ggaacgagat
     1081 agctgaaggg tatagatccc atgaagtagc cgattgctgc gaaaataagg tatctcatct
     1141 cttcctcctt agtttgagga atacaggggt accttcgtat ctatcgaagt tctgcctgat
     1201 cgtgttcctt atccctctga gataagtttc cgtgaagagg tccgggtcgt tgacgttcag
     1261 cattatcaac ggtggtctac ccgatatatg agaaccgtaa tatatctttc ctctcttctt
     1321 tcccttaccc ggcggcggag tagtttcaga atacctcgaa agcagcgtgt tcaaaagcgg
     1381 ggtaggaata cggagatcga tcttttcact tacgagttta atgctgtcta tgagcagatc
     1441 catccccttg ccgcttatgg ccgaggtgaa tatcactggg ctgtagtcga tgaaatatag
     1501 atcctgtttg actgtgcgta taagcgcctt tctccttctt tcatctatga gatcgctctt
     1561 gttgaatacc agtatagtgg ccttcccgtt cttctcggcc agtcctgcga tcctttgatc
     1621 ctggtttccc actccttcag tagagtcgat gaccatcacg atcacgtcag acctttcgat
     1681 cgcatctatg gccctcatga cgctgtagta ctcgacgttc atgactttca ctttcgattt
     1741 cttcctgatg ccggcagtat ctatgaaggt catcgctttt ccatctatgg cgagcgtttc
     1801 atctactgta tcccttgtcg taccggcgac ctccgtgaca agactccggg gacttcccac
     1861 tatccagttg aagatggacg actttcccgc attaggtcta cccacgatcg ccactttcag
     1921 agaactttgc tcttcctctt ccatgtgaac tgagtggcca atcccttcga gtctctcgaa
     1981 tatctcgtcg agaagctcgt cgatattcag gccatgggca gcggagatct ttaccggatc
     2041 gccgaaaccc agggcataga tgtcgttggc cgcggtgtaa aatgctcctt cgctctccac
     2101 tttgttggcc acgaatacaa tctctttttt ctgctttctg agataattcg agagctcgat
     2161 gtcggcgctc gtgactccct gacggccgtc aaccagaaaa atcaagagat caccctctcc
     2221 aagcagttcg agggtgacct ctttcatctt ttcttccact atattttcgg ggttttcgaa
     2281 taacccgcag gtgtccacca gctggaaggt cttctgctgc cagtttaccc tgcctactgt
     2341 aaaatcacgc gttacacccg gaatatcgtc cactatggcc cttctccctc caacgagtct
     2401 gttgaagagc gtggactttc cgacgttcgg acgtcctatg ataaggacag ttgccactag
     2461 agggtttctc cttcttcctt gagctgattc tttatcattt cacccagatt gctggagatc
     2521 gtcatgccgt tgccgttgag gtccttcatc acttcttcgg tttcggccgc ttctttgtcc
     2581 tttatcagct gtcttatcga cacaaccatg tttctcgtat cgttgtcggg atcgtaaacc
     2641 agtcttatga tcttgtactg gttgtgattc cctatactga cggcgtcttc gatattctcc
     2701 actctttcga tggaaacttg agaaacgggt aggaaggctt ccacaccgta ggagtccacc
     2761 atcactatgg ctccggactt cgtcagtctc gtcacggtac cggatatgta gtcgcctacg
     2821 gataactcgc gagagactct ctcccacggg tcggccgtag cctgtttgat gctcagtctc
     2881 attcttctgt taacgctgtc gatggagagt atcttcacct cggtctcgtc accctctttg
     2941 accacatctt ctatcctgtc aacaaagttc cacgatacct ccgatacagg aacgaagccc
     3001 gacacatact cttcaagttc gagaattacc cccgtaggaa ggactttggc cgtctttgct
     3061 ttcacgatat cgccttcgtt gtacttctga ccgatgtttt cccagggatc gcccatggct
     3121 tccctgtaag aaagggacag agtcctcttc tctatgttta tctctttgat gttgactttt
     3181 acttcgtccc cgacttcgac gacacttttg agatccttct tgatgtttcc ccagaacacc
     3241 tctgaaatat gtaccaaacc ttccacgccg ggttcaagag aaacgaagaa accgaaaggc
     3301 gttatgttct tcactatacc cgcagcggtg gaatccaccg gatatctctc ttcaagcgtt
     3361 gcccagggat cctcgctcat ctgcttgaga ctcagagaga tcttcttttc actttcgtcg
     3421 attccgatga tctttgcctt caccgtactg ccgatcttta aaagatcgct tattctcgct
     3481 cccggatccc agctcatttc cgatgccgga atcagagctg ttacgttgtc gcttagccta
     3541 acgaaggctc cgaaattctt tatcgattcc acagttcctt ccgtgatctg atcgacttcc
     3601 agcttctcga aatattcctt gatcgcttcg tcctggaagg ctcttagcga aacgacgacg
     3661 ttaatgccgt ttctctgtct cttgaatctg agcaccttga atttcaaatt gccttccggg
     3721 gcgttctcgc cctttcttat ccccgactct gaacccggga gaaatgcggg aacggttctc
     3781 agtagcctta tattgtagcc ggcgttcgtc tcggaaacta ttctgccttc tattggcgtt
     3841 ccttccctga aagccttttc cactctactg agcgcctgtc tcgcgtggac ccttttttca
     3901 gatacagtgc ttcttccctc ttcttcgttg gtctttatga ccatgacctc tatcttatcg
     3961 ccaacactgt attcgttaag gtcgtttacc agttgatcca ggggaacaaa accatctagc
     4021 ttgccttcca tgatgaccat aaggccatcg ggctggattg agaaaacctc agcttccttt
     4081 ctactgccct tcctagccga ggagacatcc atttgctcga agatctcctc catgctcagg
     4141 ttttcatctt gccgttccag tttctcttcc attgtcggtt acctccctct cgttgtctat
     4201 gtaatccagt atcttctgga tctgatcgta cggtgtcgaa gttccactta tcaaaccggt
     4261 cctccccagc tctccctgcg ggagatcccc ggtggtttgg acgtgtatcg ctctctttcc
     4321 aagtcttccg actatgtcta gaagcttttt ggtgttggag ctgtttttcc cgccaaccac
     4381 aattactgta tcggcctcgc aggccagcct tctggcttcc gattcgcgct cgacggtgat
     4441 tctacagatc gtgttgtgta tctccacgcg atctccaagt tcctcttcta ggaaagatga
     4501 gaaatctttg aagtcgtcgg tgctcatcgt agtttgggag acgacagcga ttcgctcgat
     4561 cgctttcaat ctatcgacga cggagctgta gtcttccccc ggctccacta tgaggtaccg
     4621 ctcgagtctt cccgaaagag cggtgacctc cgggtggttt ctcttcccaa atacaacaac
     4681 gaaacttccc cgggcttgaa gatcaaccgc aaggctaaat acattgtaca ctataggaca
     4741 tgtaagatct atcaccccgg taaaaacctc gcgtaatctc ttctccgatc ccgggtcgat
     4801 accatgcgct cttatgatgg ctataccatc cacacggtcg tagctggaga tatcctcgat
     4861 tatttccagg ccgaggtctt tcagattttt catgacggct tcgttatgta cgatctcgcc
     4921 ggtcgcgaaa acgctttttc cgcttttgag aatctttatg gcttcattca cggcccggtc
     4981 aacgccgtag cagaatccca cggacttcga gagcaacact ctctgtcttt ttcttgccat
     5041 agccgccact ttttccacga cttcggagat actcaaaccg gtcgtgtcga gtcgaattgc
     5101 atcttttgct ggttttaagg gagccagttc tctggaagaa tcgtttaggt ccctggcttt
     5161 catttcccgg agaaccgatt cgtaatctac ctcctttaac gaagctatga gctcggcgta
     5221 tcgcctccga gccctctcct cgatcgaggc cgtcaggaag attttcagtt cggcgtcggg
     5281 aaggactatg gtaccgatgt ctctgccctc catcaccacg tttccctgcc tcgagaggct
     5341 tctctgttgt tcggtaagat aagccctcac gtaagggtac cgggcgatgt ttgacgagag
     5401 catcgaaact tcgcgcgtcc ttatctcttc tccgagaaat ctgccgtcga gagtaatccc
     5461 gttctccgaa agagagaagt aagtatggtt catcagttca accatggggc tttcatcgga
     5521 aaactctatt ttgagactat gagctttcag cgctagggcc ctgtacatag ctccggtgtc
     5581 tatatagacg aagttcaaat ctcgtgcaac aagtttcgct acggtggact tcccggaacc
     5641 ggcaggtccg tcgatagcga ttctcactcc cgatcgcccg aatctttcaa ctgtagtttc
     5701 agtaggtcca gttgtttctg agagac
//
LOCUS       DAOOLY010000156         5706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16943_length_5706_cov_4.678818, whole genome shotgun sequence.
ACCESSION   DAOOLY010000156 DAOOLY010000000
VERSION     DAOOLY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16943_length_5706_cov_4.678818"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLD15_08200"
     CDS             complement(<1..513)
                     /locus_tag="PLD15_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63928.1"
                     /translation="MLPEGVNFCDSRNYDFLSSLFRLQNNELIMERFKRLPPSLEMEP
                     NERILKWVEKTRKTLPEGIREKLEIFFDRESFFALFLTNHIAAKKLMEPESLLSYLST
                     MSDRDLIVNFLCSGHGFSGEADLLKPISASDDERELIEYISKSMAMPTKQKADLLKLC
                     NDPSAARKELI"
     gene            complement(598..1305)
                     /locus_tag="PLD15_08205"
     CDS             complement(598..1305)
                     /locus_tag="PLD15_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB63929.1"
                     /translation="MYDRQEIARAIELRITQRKFENRRLQESEIASVRDSMNGIEHLE
                     SAGPLQWYYSCNFPIASGIVLAKMKDFSTEKLVEYGFEGEQIVLNLTLKGFGTSWARL
                     PNYLSMIVLGYPEKNEITDIERAARHLYRNSNRKPLEELITGRTELLDSEMREILLSG
                     RLAPSSFNRQPWIFEILGEREIAIHGWKKLPAIYEEVISIDLGVVLSHVYLMTRAIKD
                     EVALERKSSRTYLLRWS"
     gene            complement(1383..3725)
                     /locus_tag="PLD15_08210"
     CDS             complement(1383..3725)
                     /locus_tag="PLD15_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPB63930.1"
                     /translation="MEWFKDFSILVVDDDLRSENTGGRATREIIKELQKRGLSVIESY
                     SGYDCKIEFMSHSNISCILLDWDLVIRSDEEFLGPGEIIEIIRSKNALIPLFLMTEKL
                     RVKEIPLEIVSQIDGYVWKLEDSPSFIAGRIEEAIERYMEELLPPFLKELIHYVDEFK
                     YSWHTPGHSGGEAFLKSPTGKLFHRFFGENVFRSDLSVSVPELGSLLDHTEAIGAAEK
                     FAAKIFGADETYFVTNGTSTSNKIVFHSCVTPGDIVLIDRNCHKSIMHSIIMTGAIPI
                     YLTPSRNSLGIIGPIHEEEFDWAEIEKKINSSSLVSDKENFRIKLAVVTNSTYDGLCY
                     NTRKIIDRLEGVVDYVLFDEAWYAYANFHPMYVGRYGMSPEIDRERAPTVFTTHSTHK
                     LLAAFSQGSMIHVKDGKKKIDHGRFNEAYMMHMSTSPQYAIIASLDVSSKMMAGNSGR
                     FLIDETIQEAIVFRKKMKHLSEEIEDKEIDRKRRWWFEIWQPSQITMENEKGERKRED
                     IEDIDEQLLKDHPEYWCLKGNEDWHGFGKLESDYILLDPIKVTVMTPGITKQGRMRNW
                     GIPATIVTTFLRDRGIVVEKSGHYSFLILFSLGLTKGKSGTLLAELFTFKKLFDENAP
                     LDDVFPDIVKKHPKKYGKMTLQELSEQMHEYLRKARIARVLKDVYSQAPEQVMLPARA
                     YSELVNGNTELVRIRELQNRISAVMVVPYPPGIPVIMPGERYTPETKRIVEYLNFSED
                     FDNRFPGFENEMHGLKVKLDSNNKKRYYTYCLKETPQEST"
     gene            complement(3741..4589)
                     /locus_tag="PLD15_08215"
     CDS             complement(3741..4589)
                     /locus_tag="PLD15_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPB63931.1"
                     /translation="MKIDPRCIPCNMSALVRILSQNGVKREEQLVAAGLFLKDMAEMD
                     LEAETPIEIGRNVGKALKAVMGEIDAFREVKKAADDLLIEVYNELKEKLRALEDPLIG
                     ALKLSVAGNIIDFAPGHRIDIDSTMRQALAAPFAIDDLEELSERIKGAKSMLIVGDNC
                     GEVVLDKLLMEIAGVPETYFAVRSKPALNDVTMKEALEVGLDSVATLIDSGADAPGMQ
                     EDRMSDEFEDIYTGADVVISKGQGNLEGLSSAKREIFFLLMAKCEVIGSFLNVPKGSF
                     VAYRKA"
     gene            4901..5434
                     /locus_tag="PLD15_08220"
     CDS             4901..5434
                     /locus_tag="PLD15_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPB63932.1"
                     /translation="MKSIVIYFSFDESTRTLAEAMASSIGADLLRLVPAGDFARTYYH
                     HVENPEQIRSTPGYNSEREHVWGSESVTMNNKPELEPYDFDPGLYEMIIIGTPVWALT
                     YAPPFNTFLEKAAIKGKRIALFCTHQGIIGAALEDLKKALKGNEIVQEIDFESPASRM
                     DEYVETSREWARELLHL"
     gene            5546..>5706
                     /locus_tag="PLD15_08225"
     CDS             5546..>5706
                     /locus_tag="PLD15_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB63933.1"
                     /translation="MKVLLTNDDGIEAAGIRLLAKRLALTHDVTVVAPEGNRSGVSHS
                     ITWLTPVRIR"
BASE COUNT         1332 a         1431 c         1362 g         1581 t
ORIGIN      
        1 tatcagctct tttctggctg cagacggatc attacagagc ttcaagaggt cggccttttg
       61 tttcgtcggc atggccatgc ttttagatat gtactctatc agctctcgct cgtcatcgct
      121 ggccgaaatc ggcttcaaaa gatcggcctc tccggagaat ccatgaccgg agcagagaaa
      181 gttcactatc aggtccctgt cggacattgt tgaaaggtag gaaagtagag actccggttc
      241 catcaacttc ttagctgcta tatggttggt caggaagagg gcgaaaaaag actccctgtc
      301 gaagaagatc tcaagttttt ctctgattcc ctcgggaagt gtttttcttg tcttctcgac
      361 ccacttcagg attctctcgt tcggttccat ttcgagactg ggaggtaacc ttttgaatct
      421 ctccattatc aactcgttgt tttggagtct gaaaagagag gatagaaagt cgtagtttct
      481 tgaatcgcag aaattcacac cttcaggcaa cacaaaaatt cctccattcc aggaagttga
      541 tccggtatat tatacatcag tcgaagtcga tgcacagata tccgggccga agagacttca
      601 ggaccatctg agcagataag ttctggagct ctttctctcc agggcaacct cgtccttgat
      661 ggcgcgcgtc atcagataga cgtgggaaag taccacaccc agatctatcg atatcacttc
      721 ctcgtatatg gctggcagct tcttccagcc gtgtatggct atctctctct cacccagaat
      781 ttcaaagatc cacggctgac ggttgaacga agagggagca agtcttccgg aaaggagtat
      841 ttcccgcatt tcagaatcca gcaattccgt tctgcctgtg atcaattctt cgagtggttt
      901 tcgatttgaa ttccggtaaa gatgccgtgc agccctttct atatccgtga tctcattctt
      961 ctcgggataa cctagaacga tcatggagag gtaattgggg agtcttgccc agctggtgcc
     1021 gaaacctttg agcgtgagat tcaaaactat ctgttcccct tcgaagccgt actccacaag
     1081 cttctcggtc gaaaaatcct tcatctttgc cagaactatc cctgacgcga tgggaaagtt
     1141 acacgaatag taccactgga gaggtccggc gctctcgaga tgctctattc cattcataga
     1201 atctctcacc gaagctatct cagactcctg gagccgtctg ttttcgaatt tcctctgtgt
     1261 aatcctgagt tctatcgctc tggcgatttc ctgtctgtcg tacataaaat caccccttta
     1321 ctgtatttta cataatacag ataataaaac tcaagattat tccactcaca gattcgtgcc
     1381 aattatgtgg attcctgagg tgtttctttc aggcaatagg tgtagtatct ctttttgttg
     1441 ttgctgtcca gcttcacctt gagaccgtgc atctcgtttt cgaatccggg gaacctgttg
     1501 tcgaaatcct cggaaaaatt gagatactcg actatcctct tcgtttcggg cgtgtacctc
     1561 tcaccgggca tgatcaccgg tatgcctggg ggatacggaa ctaccattac ggcagatatc
     1621 ctgttctgaa gttcccttat cctgaccaat tcggtgttgc cgtttactag ttccgagtag
     1681 gctctggcag gaagcatcac ctgttcgggg gcctgtgaat agacgtcttt caaaaccctg
     1741 gcgattctgg cctttcgaag gtactcgtgc atctgttcgg acagctcctg gagagtcatt
     1801 ttgccgtatt ttttcgggtg tttcttgact atatccggga agacatcgtc tagaggggcg
     1861 ttttcatcga acagcttttt gaacgtgaaa agctctgcca gcaatgtacc agattttcct
     1921 ttggtgaggc ctagcgaaaa gagtatcagg aaggaatagt gtccggattt ctcgaccact
     1981 atcccgcggt ccctgagaaa agtggtcact atggtggccg gtatccccca gtttctcatt
     2041 ctgccctgtt tggttatccc cggtgtcatc acggtcactt tgatggggtc gaggagtatg
     2101 taatcgcttt ccagctttcc aaagccgtgc cagtcctcat tccccttcaa gcaccagtac
     2161 tcgggatggt ccttgaggag ttgttcgtcg atatcttcta tatcttctct tttcctctca
     2221 cccttttcgt tctccatggt gatctgtgaa ggctgccaga tctcgaacca ccatcttctc
     2281 ttgcgatcga tctctttgtc ctctatctct tcgctcaggt gtttcatctt cttcctgaag
     2341 acgatcgcct cctgtattgt ttcgtctatt aggaaccgac cggagtttcc ggccatcatc
     2401 ttcgaggaaa cgtcgagcga ggcgattatt gcgtactgag gagaggtcga catatgcatc
     2461 atataggctt cgttgaatct cccgtgatct atcttcttct ttccgtcttt cacatgtatc
     2521 atcgaaccct gtgaaaaagc ggcgagaagt ttgtgagtcg aatgagtcgt gaaaacggta
     2581 ggagccctct ctcggtctat ttccggggac atcccgtacc tgcccacgta catcggatga
     2641 aaattggcgt acgcgtacca tgcctcgtcg aagagaacgt aatccacaac gccctcgagt
     2701 ctgtcgatta tcttcctggt gttgtaacag agtccgtcgt acgttgaatt cgtcacgact
     2761 gccagcttga ttctgaagtt ttctttgtca ctgaccagcg aactggaatt gattttcttt
     2821 tctatctcgg cccagtcgaa ctcctcttca tgaatgggac cgataatgcc gagcgagttt
     2881 ctactgggtg taaggtatat cggaatagcg cctgtcatta ttatcgagtg cattatagat
     2941 ttgtggcagt tcctgtcgat gagaacgatg tcgccgggcg tgacgcatga atggaagaca
     3001 atcttgttgg acgtggatgt tccgttggtg acaaagtacg tctcgtcggc tccgaagatc
     3061 tttgccgcga acttttcggc cgcgccgatg gcttcggtgt gatcgaggag agatccgagt
     3121 tcggggaccg atacagagag atcgctgcga aagacgttct ctccgaaaaa cctgtgaaag
     3181 agcttcccgg ttggggactt caaaaaggct tcgccaccgc tgtgtcccgg ggtgtgccaa
     3241 gagtatttga actcatccac gtaatggatc agctctttga gaaagggcgg tagaagctcc
     3301 tccatgtatc tctcgatagc ctcttcgata cgaccggcta tgaaagatgg actgtcttct
     3361 agtttccata cgtaaccatc tatctgactg actatctcga ggggaatctc tttgaccctg
     3421 agcttttcgg tcatcaggaa gagcggtata agggcgtttt tgctcctaat tatctctatg
     3481 atctctcccg ggccgaggaa ctcttcatcg gatcttatca cgagatccca gtcaagcaag
     3541 atgcagctta tgttggagtg agacatgaat tcgattttgc agtcgtaacc cgagtacgat
     3601 tctataacgg acaggcccct tttctggagc tcttttatta tctctcgagt tgctcttcca
     3661 ccagtgtttt ccgagcgtag atcgtcgtca accaccagaa ttgagaaatc cttgaaccac
     3721 tccatatccg ctccccccga tcatgccttt ctgtaggcta cgaacgaccc ttttgggaca
     3781 tttagaaaac tgcctatgac ctcgcacttg gccatcaaaa ggaagaagat ctctctcttg
     3841 gcagaactca atccctccag attgccctgt cccttagaga ttaccacgtc ggcaccggta
     3901 tatatatcct caaactcgtc actcattcga tcttcctgca taccgggtgc gtctgctccg
     3961 gaatcgataa gagtggcgac gctgtccaga ccgacttcga gcgcttcctt catggtgaca
     4021 tcgttcaagg ccggtttgct gcgaactgcg aaatacgtct ccgggactcc ggctatctcc
     4081 ataaggagtt tatcgaggac cacttcgccg cagttgtcgc ccacgatcaa catactcttt
     4141 gcccctttga ttctctcaga tagttcttca agatcgtcga tcgcaaaggg agcggcgagg
     4201 gcctgcctca tagttgaatc gatgtcgatc ctgtggcccg gggcgaaatc tatgatattg
     4261 ccagccaccg agagcttcag agccccgatc aaagggtctt cgagcgcacg gagtttctct
     4321 ttgagctcat tgtaaacctc aattaagaga tcgtcggccg cttttttaac ctccctgaag
     4381 gcgtcgatct cgcccatgac cgcttttaga gccttcccca cattacgccc gatttcaata
     4441 ggagtttcgg cctccagatc catctccgcc atatctttca agaatagtcc ggcggcgacg
     4501 agttgttctt cgcgcttcac accgttctgg gaaagtattc ttaccagcgc actcatattg
     4561 catggtatgc atcttggatc tattttcatt acatcacctc gacggaatta tacatgccgg
     4621 atgcgaatag ataattaaga tgatcgaaag agcggggcac cagaggagag gaagagagag
     4681 aaagagagag agaaagagag agagtgaagc gaactctcga tgttctttgc cggctcttat
     4741 caataatctg ctacgcacag ccctaacaat gagtcctgaa atcgacggtt tttcagtcta
     4801 taatatatgt gagagtatct aagaggtctg tcttttctta tccagtgaga cattccattc
     4861 agcgagattt tgttagaccg ccaatgagga ggcgaagagc atgaagtcaa tagtaatcta
     4921 cttttctttc gatgaaagca ccaggacgct ggccgaggct atggcgtctt cgatcggcgc
     4981 tgatttgttg agactggtgc ctgccgggga tttcgctcgt acctattacc accacgtgga
     5041 gaatcccgaa cagattagat cgacacccgg atacaattcc gaaagggaac acgtttgggg
     5101 cagcgaatcg gtaaccatga acaacaaacc cgaactcgag ccttacgatt tcgatcctgg
     5161 gctttacgaa atgatcataa tcggcacacc tgtatgggcc ctcacttacg caccgccgtt
     5221 caacacattt ctcgagaagg cagccattaa aggcaaaagg atcgccctct tctgtacgca
     5281 ccaaggaatt ataggcgcag cgctggagga cctgaaaaaa gcccttaagg gaaacgagat
     5341 cgttcaggag atcgatttcg aatcgcccgc ctcgagaatg gacgagtatg ttgagacttc
     5401 aagagaatgg gcccgggaac tccttcatct ctgaatccaa aagcggaaag acggacgcgg
     5461 ctgaaaatgc gtttctgact cggtctctgt attggccggt tgatgggtcg atcgatactt
     5521 aaagtttatt gggtggtgaa ttcaattgaa agtacttctt accaacgatg atggcattga
     5581 ggccgcgggg attcggcttc tcgccaaaag gctcgcgctc actcacgatg ttacggttgt
     5641 ggctccggaa ggtaaccgga gcggtgtgag ccattcaatt acgtggttga ctcccgtgag
     5701 aatacg
//
LOCUS       DAOOLY010000157         5692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_16995_length_5692_cov_5.172787, whole genome shotgun sequence.
ACCESSION   DAOOLY010000157 DAOOLY010000000
VERSION     DAOOLY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16995_length_5692_cov_5.172787"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..597)
                     /locus_tag="PLD15_08230"
     CDS             complement(67..597)
                     /locus_tag="PLD15_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63934.1"
                     /translation="MKRVILAVLLAFILSSTAFGVQNTSTQELRIVVQPVALLDVSLL
                     TVVFEIPQEIVPGAPLHFENIEGGNIRYTSLIEQGRSRKITASLVDTSILDYVEIGIT
                     VAAPEKGTGKLGVSTGRISLSSIPQPVIADIGNGWTGTEETDGASVFYDIDILSADNL
                     GSGTVTIDVLFTLAEE"
     gene            complement(1096..1926)
                     /locus_tag="PLD15_08235"
     CDS             complement(1096..1926)
                     /locus_tag="PLD15_08235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB63935.1"
                     /translation="MKEPEKWKLKDVRAVFSDIDGTLLDSEERLSGENLKAIQEIVNR
                     GILMVFASGRSTGSIRNLFAGLDEHNFNAIAFNGSEVTLRGEVISRTKLDSVTASQIV
                     RTVSEINIHIHAYVDGELVFSKLTPTAERYAIHTGLEPMIVGDLAVYLQENPPIKLVV
                     LSEPGYLPSLREKLAGQFPEVSFTRSGGIYLDVTRSNVDKGRGLIEICEREGIAPENA
                     VAFGDSDNDIEMLKAAGLSVAMSNGSEEVRRIATVTTVSNDENGFSKMMKKLLRNMTP
                     "
     gene            complement(1932..2765)
                     /locus_tag="PLD15_08240"
     CDS             complement(1932..2765)
                     /locus_tag="PLD15_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63936.1"
                     /translation="MKRGNLVSKATVYLVLVIFSLMILMPLIWLTTTAFKTKTEIFSN
                     PFNILPGSPDFGNFKNAWSYAPFASYYLNTIITSFGLLAIQLVTITMAAYAFARLQFP
                     GRDFFFILFLTQLMITPQSTILPNYLTISQMGLIDTRLGVMLPYFASAMGTFLMRQAF
                     KSVPESLEDAGKLDGCNTFQLIWHVFLPIVRPSLIAFSIISVTYHWNEFLWPLLVTET
                     SRSRTLTVGLTIFAQQAEGGAEWSLLMAATLIVIGPLLIAFTIFQKLFVQSFANSGMK
                     G"
     gene            complement(2770..3657)
                     /locus_tag="PLD15_08245"
     CDS             complement(2770..3657)
                     /locus_tag="PLD15_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63937.1"
                     /translation="MRLSRKWRTYLLGYLLILPSFVFLIVFTYYPVVRSVWLSLFQKM
                     AGGVTKFVGLANYEYMFGSRLFRQVMQNNLVYSLGSVIPAIILGLVFAILLNKRLKLR
                     GAFRFSIFYPTMIPIAAASMVWIFLFSQSYGLLNKVLSLLGLSSNIDWLNTTPYAMLS
                     IILVSVWKFAGYYMLLFLSGLQSIDESLYEAAVLEGANAWQKFRKITLPLLSPTTFFV
                     TLMAIINSFQSVDQVYVMTRGGPYNTTNVLLYYIYQNGFVYWDTGIASSASVVLFSIL
                     LVFTFIYYFGLQHFVHYER"
     gene            complement(3722..4990)
                     /locus_tag="PLD15_08250"
     CDS             complement(3722..4990)
                     /locus_tag="PLD15_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63938.1"
                     /translation="MRKFFVIALVVFAVFATSLIAADPVILRFYFPVGVAGPLARYMG
                     ELADKFNSTHPDIIVEPIYSGGYPETLQRALTASKAGNPPDVALLTAADIWTAADEKI
                     IISLESFIEAEGGETFLEPYFQAFLEDCDVAGELYAIPFQKSTPIFYYNKDMFREAGL
                     DPDRPPSTWTELKEYATKLAKKEGGNTVRWGVEIPIDQWLLSAFIMQNGGLVNNQAGT
                     ETYLNSPEAIGALEFMRSLVAEGLMPARRLFGDSSADFVAGTTAMMYNSTGSLTFVRD
                     SATFDWGVAYLPENVRRAVPTGGGQLVIMAGIPAERQKAAWEFIKWMTLPEQAATWSM
                     KTGYVAVREDAFDVPEMKAYVEGFPFAIVAREQLQFVLVGEPPATHAARQISRFMTDA
                     LEGAIAGMISAKDALNTAQAEAERVLRPYR"
     gene            complement(5021..>5692)
                     /locus_tag="PLD15_08255"
     CDS             complement(5021..>5692)
                     /locus_tag="PLD15_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB63939.1"
                     /translation="DRVNNLNYEKDRENALKFARIMSSLSVPYYPILGNHDIHYLTKE
                     ENREIFDRPVENRVAFVKGYKLIFLDTEEPVIEGVGGSVSNEQLKWLERELNSDDLSK
                     IIFAHHPADDQEIRDNPHFVQFPQLAFVENREKVRETVGRGKNVLAYINGHVHWLGLY
                     ECNRIPYISVPSFTEAWPEKSGAPGMFAQVFISENGRLELTIRSLNPRRILGKFFWNS
                     TCSSL"
BASE COUNT         1413 a         1456 c         1387 g         1436 t
ORIGIN      
        1 acgatcgact tcaaaatgag gaagaagagc gaacagataa tcataataga ttccggtgta
       61 ctctgatcac tcctcggcga gtgtgaagag cacatctata gttacggtac cgcttccaag
      121 gttgtccgcc gacagtatat cgatatcgta gaaaacactg gcaccatcgg tctcttccgt
      181 tcccgtccag ccgttgccta tatctgcgat aaccggctgc ggtatcgagc tgagagagat
      241 ccttcccgtg gatactccca gttttccagt tcccttttca ggcgccgcca cggttattcc
      301 tatttcaaca tagtcgagga tcgacgtatc taccagcgaa gccgttatct ttcttgacct
      361 gccctgctcg atgagcgagg tgtatcttat attcccgcct tctatgtttt caaaatgcaa
      421 aggtgcaccg gggactatct cctgggggat ttcgaagacc accgtcagga gagatacatc
      481 cagtaatgcc actggctgca caactattct aagctcctgg gtagatgtgt tctgtactcc
      541 aaaagctgtc gaagataaaa taaaagccag caagactgct aaaatcactc ttttcatcta
      601 tttcatcccc tcataaatga gcttctcgaa catattttac cattaaaacc gataaaaata
      661 atctatggat tatattatta tattaaatca tatgattgaa taagaaagat caacaggagc
      721 cacagtgggt ggtggatagt gagtagtggt tagtgaatgg tgtgttgtgg gtaagaatcg
      781 agaaagagcg tcaagagtta accaggagcc gttatccgtc gtccgtccac cgccctccga
      841 gaagagcaag agcgggatgc cggatcgcga tccggcatga cgggaaggga gtaagactgt
      901 catcccgtag tgatcctatc acgagaagac gagagcgaaa aacacgagag aaagaacgtt
      961 gagagtccgc ttcactcacg agaaggcgtc tcgctctctc tacttctctc tcttactctc
     1021 tcattctctc ttttcctctc tcttcctctc ctctgacctc tgttttacca ggaacctgga
     1081 cgcgaaagcc ttgagttatg gggtcatatt tcgcagcaat ttcttcatca tcttcgaaaa
     1141 tccgttttca tcgttcgata cagttgttac agtcgcgatt ctccgcactt cttcggaacc
     1201 gttagacatg gccacggaaa gaccggccgc tttaagcatt tcgatatcgt tgtcactatc
     1261 tccaaaggct acggcgtttt cgggcgctat accttctctt tcgcatattt caatcaatcc
     1321 acgacctttg tcaacgttcg agcgcgtgac atccaggtag attcctccgg atctggtgaa
     1381 actgacctct ggaaactgcc cggcgagctt ctccctcaaa gagggaagat agccgggttc
     1441 tgagagtacg accagcttga tcggcgggtt ctcctggaga tatacagcaa gatctccaac
     1501 gatcatcggc tctaagccgg tatgtattgc atacctttcc gccgtagggg tcagtttcga
     1561 aaagaccagc tcgccatcca cataagcgtg aatatgaata ttgatctcac ttacagtcct
     1621 gactatttgc gaagccgtta cggagtctag cttagttctg gaaatcacct ctcccctcag
     1681 ggtaacttca gaaccgttga aggcaatggc gttgaaatta tgttcatcga gtccggcgaa
     1741 gaggtttctg atcgatccgg tcgatctacc gctggcaaaa accattaaaa tgccccggtt
     1801 cacgatctcc tggatcgcct tcaggttctc accggacaat ctctcttcgc tgtctagaag
     1861 cgtgccgtct atatcactga aaacggcccg cacgtctttt aacttccact tctccggctc
     1921 tttcatggag gtcatccttt catcccggaa ttggcgaagc tctgaacgaa aagcttctga
     1981 aatatagtga acgcgatcaa aagaggccct atgactatca gagtcgctgc catgagcaaa
     2041 ctccattcgg cccctccctc ggcctgctgg gcgaagatcg tcagacctac cgtcagggtt
     2101 ctcgatctgg acgtttcggt aaccaggagc ggccagagaa attcattcca gtgatatgtg
     2161 acgctaatta tcgaaaaggc tatcagcgaa ggccggacga tcggcagaaa gacgtgccag
     2221 atcaactgaa aggtgttgca accgtcgagt ttgccggcat cctccagaga ttccgggacg
     2281 cttttgaaag cctgtctcat caaaaaggtg cccatggccg aggcgaagta cgggagcatg
     2341 acccccagcc tggtatcgat caatcccatc tggcttatgg tcaggtagtt tggaagaatc
     2401 gtgctctgcg gggtgatcat cagttgcgtg aggaagagta tgaaaaagaa atcccgtccg
     2461 ggaaactgga gcctcgcgaa ggcgtaggct gccatcgtta tcgtaactag ctgaattgcc
     2521 agcagtccga aagaggttat tatagtgttc agatagtagc tcgcaaacgg cgcgtatgac
     2581 caggcgtttt tgaaattgcc gaagtcagga ctgcccggca aaatgttgaa agggttggag
     2641 aagatctcgg tcttcgtctt gaaagccgta gtcgtcaacc atatcagagg catcaggatc
     2701 atgagcgaaa agatcaccag tacgaggtaa accgttgctt tcgatacaag atttccccgt
     2761 ttcatacgat cacctctcgt agtggacgaa gtgttgcagt ccaaagtagt aaatgaaggt
     2821 gaaaaccagc aggatcgaga agagaaccac cgacgcgctg ctggcgatcc cggtgtccca
     2881 gtaaacgaaa ccgttctggt agatgtagta gagtaagacg ttggtggtgt tgtaaggtcc
     2941 tcccctggtc atgacatata cctgatcaac ggactggaag gagtttatga tcgccatcaa
     3001 agttacgaaa aaagtcgtcg gagagagtag tggcagggtt atctttctaa acttctgcca
     3061 ggcgttggca ccttccagca cggccgcttc atacagactc tcatcgatgg actgaagccc
     3121 cgatagaaag agcaacatat aatagccggc gaatttccaa acagagacta ggatgatcga
     3181 gagcatcgcg taaggggtgg tgttcaacca gtcgatgttc gacgagaggc ccagaagact
     3241 cagtactttg ttcagcaatc cataggactg gctgaaaaga aagatccata ccattgaggc
     3301 ggccgcgatt ggaatcatag tggggtagaa tatcgaaaat cggaaagccc ctcgaagctt
     3361 caaacgtttg ttcaggagta tcgcaaaaac cagtccgaga attattgccg gtatgacgct
     3421 tccgagtgag taaacgagat tgttttgcat tacctgcctg aagagacggc ttccaaacat
     3481 gtattcgtag ttggccagac cgacgaactt cgtgactcct cccgccattt tctgaaagag
     3541 gctcaaccat acggagcgaa ccaccggata gtaagtgaat actatgagaa atacgaagct
     3601 tggaagaata agcaaataac cgagtaaata agttctccac ttacgggaca atctcattct
     3661 ctcacccccg attcaaaaaa ggaaaggagg agcaagtttc gcgctcctcc ttccacgctt
     3721 cttatctgta aggtctcaga actctttcgg cttcggcctg agccgtgttc agagcatctt
     3781 tcgcgcttat cattccggcg attgccccct ccagtgcatc agtcatgaag cgggagatct
     3841 gtcttgccgc gtgagttgcc ggcggttcac cgaccagaac gaactggagc tgttctctcg
     3901 cgactatcgc gaagggaaat ccttcgacgt acgctttcat ttcaggaaca tcgaaggcat
     3961 cctctcttac ggccacgtac cctgtcttca tgctccaggt tgcagcctgt tcgggaagcg
     4021 tcatccattt aatgaattcc cacgccgcct tctggcgttc tgccggtatt cccgccatta
     4081 ttacaagctg cccaccaccc gtcggaacgg cccttctgac gttctcggga agataggcca
     4141 caccccagtc gaaggtcgcg ctgtccctga caaaagttag actgcccgta gagttgtaca
     4201 tcatggccgt agtgccggcg acaaaatcgg cagaagagtc tccgaagagt cttctggcag
     4261 gcataagacc ttcagcaact agagatctca tgaactcgag agccccgatc gcttctgggc
     4321 tgttgagata ggtttccgtt ccggcctgat tgttcacgag tccgccattt tgcattatga
     4381 aggccgagag caaccactga tctatcggga tttccactcc ccagcgcacg gtgttcccgc
     4441 cttccttttt cgcgagtttt gtcgcgtatt ctttcagttc cgtccatgtc gaaggcggtc
     4501 tgtcgggatc gaggccggcc tctctgaaca tgtccttgtt gtagtagaag atcggagtgc
     4561 ttttctggaa tggtatcgcg taaagctctc cggcgacgtc acagtcctcc agaaaggcct
     4621 ggaagtaggg ttcgaggaag gtctctcctc cttcggcttc gataaacgat tcgagagaga
     4681 tgatgatctt ctcatcggcc gcggtccata tgtcggcggc ggtgaggaga gccacatccg
     4741 gcgggttccc ggcctttgac gccgtcagag ccctctggag agtttccgga taaccaccgg
     4801 agtatatcgg ttcgacgatg atatccgggt gggtactgtt gaacttgtcg gccagctctc
     4861 ccatgtacct ggccagcgga ccggcgacac ccacagggaa gtagaatcta agaattaccg
     4921 gatcggctgc gatcaacgat gttgcaaaca ctgcaaaaac aactagagca attacaaaga
     4981 acttacgcat gcatttcccc tccttttctc tggttgattc ctataaagaa ctgcacgtgc
     5041 tgttccagaa gaacttcccc aggattcttc gaggattcaa gcttcgtatg gtcagttcca
     5101 gacgaccgtt ttctgaaata aaaacctgcg caaacattcc gggggcgccg ctcttctccg
     5161 gccacgcttc cgtaaaagac ggtactgaga tatacggtat tcgattgcac tcgtacaggc
     5221 ctaaccagtg tacatgcccg ttgatataag ccaagacatt ctttcctcta ccaaccgtct
     5281 ctcttacttt ttctctgttc tccacgaagg ccagttgcgg aaactgaacg aagtgagggt
     5341 tgtctcttat ctcctgatcg tcggcgggat ggtgggcaaa gattattttc gacagatcgt
     5401 cggaattcag ttcgcgttca agccacttca gctgttcatt agagacactt ccgccgaccc
     5461 cttctataac cggctcttcc gtatccagaa atatcagctt gtaacccttc acgaaagcga
     5521 ctctattttc aaccgggcgg tcgaatatct ccctgttctc ttcctttgtg aggtagtgta
     5581 tatcgtgatt tcccaggatc gggtaataag gtaccgatag agaactcatt atgcgggcga
     5641 atttcagtgc gttttcacgg tctttttcgt agttgaggtt gtttactctg tc
//
LOCUS       DAOOLY010000158         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_17070_length_5672_cov_4.561688, whole genome shotgun sequence.
ACCESSION   DAOOLY010000158 DAOOLY010000000
VERSION     DAOOLY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17070_length_5672_cov_4.561688"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLD15_08260"
     CDS             <1..1035
                     /locus_tag="PLD15_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB63940.1"
                     /translation="RKWTNLCLLILAAAGTGLTGFAPSMRLFSVFRFLSGLGYGGLLP
                     VVNAYLTEFSSIKIRGLYLTLLESSWALGSIMVGAFTMLTLDSLGWQWSYYFLFIFGI
                     PLIMIGLFLPESPKFEFMKKGKKALEKILKTSIKEEVEMHEREKQPLLSIFKKGLARR
                     TLMIWFSWFTVSFVYYGIYTWAPKIFASKGLTPVSSLWYTFFMLIMQLPGYLTAAFLI
                     EKIGRKTTLTFFFVATAISALIMGLVTSSGFLIFASILISMSVLGTWGMVYAYTPELY
                     PTEMRGLGNGTSGVMARTAGIIAPYFTNFFMGRTGSVLYVMIFMSVMSVLAAIIVALF
                     GVETKEKIIE"
     gene            1099..1851
                     /locus_tag="PLD15_08265"
     CDS             1099..1851
                     /locus_tag="PLD15_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPB63941.1"
                     /translation="MAMRPEDVLFMMILFGRGIVSSGGSATDVEVALESIGKKNGFKT
                     EVASTPTVIHISLIEPDGRSWSRIERIKVERPDFYRITAYRNLLREYLDDETSWVDIV
                     ESLKLLESTRERYPLLLRSICSGIAAMAFSYVFGGNLLESVFSGVITAPIFLLLSLIP
                     SNRMLIDFLAGFMTTILAVLCGYFFDLNSSKIIVGSIMPYVPGILITNSILELANRNL
                     VSGSSKFTEAILILVSLVFGASSAFFVAGGLV"
     gene            1848..2294
                     /locus_tag="PLD15_08270"
     CDS             1848..2294
                     /locus_tag="PLD15_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPB63942.1"
                     /translation="MILRVLASLIAGAGFAIIFSVPAGIIPLASCVAALGWLVYEFTS
                     GLLGLALSVFLSSLIIGILSRLIATLKHWPLQPLIVVGIVPLVPGSSAFYAMQSFMSE
                     DLFRAFEYSYRTFSSASGIVIGLVASSTVFNIIEKIIELSNKNNNI"
     gene            2548..2931
                     /locus_tag="PLD15_08275"
     CDS             2548..2931
                     /locus_tag="PLD15_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HPB63943.1"
                     /translation="MLSLKRRLTKCPVCDGDLKITEYTCPSCKITIRGDFYQDEIVSL
                     SEELLDFLKVFIKNRGNLSELQKELNISYPTARSKLEELVTSLGYEVAGRQVQEASKI
                     IKKLEDGLIEPEEAVKLLNRMKGKR"
     gene            complement(2906..3934)
                     /locus_tag="PLD15_08280"
     CDS             complement(2906..3934)
                     /locus_tag="PLD15_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB63944.1"
                     /translation="MTSRERVFARIEGRSVDQVPNLNIIMAFAAKYIGKTYREYATDF
                     RVLCEGNIRCCEDFGIDMVSAISDPAREVNDLGGEVIFPGDDIPYFGLPLLSAAEPLK
                     LKVVEPSTGRRMSDRIGAIDFYNNHASAEYPVLGWVEGPLALAADLRTPVGIMEDFFE
                     RPEFVVELLEFCLEQETIFALEQVRAGADFIGIGDAICSVIGPRSYSKFGLPYEKLLI
                     KRVHEAGAKVKLHICGDTTPILSLMINTGADIIDVDWMVDFASAVELGGGKISICGNF
                     DPVSVMFKGSVEKVADSVEKCLDVSDGRTFIAAGCEIPRGTPLENMAQVKNVLKARGG
                     FNACPSYG"
     gene            4069..4974
                     /locus_tag="PLD15_08285"
     CDS             4069..4974
                     /locus_tag="PLD15_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB63945.1"
                     /translation="MKAIFTVLFVLLIAVIVHAIFSVTMARIYLGRMDQDGEYITVGE
                     EKLYIEVSGAGDPIILIHGFLGSYLDFDAIVDGLSTFRKVYVIDLPGFGLSRASLEGD
                     YSKKGYADLVSGLMHILNLNLADVLGHSMGGEVALNLAYYHPERVKSLILVDSMGYSK
                     MNFLPSLVEDNEFLNHVLMRYGFQTYLVQRFLYRQKLGDVSKFNRESFHLSFSLINHI
                     SPAFLYRFNLQDDSGALSGKIRDIKSRTLIVWGEKDRIAPVTDGERFKIDIPGSELEI
                     ITGVGHSPMLENPSLFLEKLKLFLK"
     gene            4991..>5672
                     /locus_tag="PLD15_08290"
     CDS             4991..>5672
                     /locus_tag="PLD15_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPB63946.1"
                     /translation="MAVKRQEAFMGKFARPEVVVSRCLGFESCRYNAQTVPSNFVSTL
                     GNFVNYTTVCPEVDIGLGVPRAPIRLIALDSVVRLIQPSTGLDVTEKMNAYASSLLSS
                     LENVDGFILKALSPSCGIKDVKVYPGAGKVMATGKDSGIFGGEVVTRFGHLPVEDEGR
                     LTNFVLREHFLTTLFTVTRFRELNLNRSIKGLVDFQSDNKLLFMSYNQNEMRAMGRIA
                     GNLEKKDLN"
BASE COUNT         1380 a         1333 c         1389 g         1570 t
ORIGIN      
        1 agaaagtgga ccaacctttg tctgctgata ctcgccgcgg caggaaccgg tctcactgga
       61 ttcgccccat cgatgagact gttctcggtg ttcagatttc tttccggtct gggttacggt
      121 ggacttcttc ccgttgtcaa cgcctatctc acagagtttt cttcaatcaa gataaggggg
      181 ctctacctca cgcttctaga atcgagctgg gctcttggaa gtatcatggt tggagccttc
      241 accatgctta cgcttgattc cctgggctgg caatggtcat attatttcct cttcattttc
      301 ggaatccccc tgataatgat cggcctcttc cttccagaaa gccccaaatt cgagttcatg
      361 aagaagggga agaaggccct ggagaaaata ctcaaaacct ccataaagga agaagtggag
      421 atgcacgaaa gggaaaagca accccttcta agtatcttca aaaaaggtct ggcaagaaga
      481 actctcatga tatggttcag ctggttcaca gtaagcttcg tttattacgg aatctacacc
      541 tgggcgccca agatattcgc ttcaaagggg ttaacccctg tctcatcgct gtggtacact
      601 ttttttatgc tcatcatgca gcttccgggc taccttacgg ctgccttcct catagagaag
      661 atcggaagaa aaaccaccct gactttcttt ttcgtcgcca ccgcgatttc ggcgctgata
      721 atggggttgg ttacgagttc gggctttcta atcttcgcaa gcattctgat ttctatgtct
      781 gtcctcggaa cctggggaat ggtttacgct tatacaccgg aactctatcc aacggagatg
      841 aggggattgg ggaatggcac ttccggagtg atggccagaa cggccggcat aatcgccccg
      901 tatttcacga acttcttcat gggccgtacg ggcagcgttc tatacgtaat gatcttcatg
      961 tcggtcatga gcgttctggc tgcgataata gtggctcttt ttggagttga gacgaaagaa
     1021 aagataatag aatgatctcc tggcgacttc ccttgaggat cttatgggtg gtagtattgt
     1081 cttcgggagg ttttggagat ggcgatgaga cctgaagatg tgctattcat gatgattcta
     1141 ttcgggaggg gaattgtgag ctccggaggt tccgcaaccg atgttgaagt ggccctggaa
     1201 tcgatcggca aaaagaacgg cttcaagacc gaggttgctt cgacacccac ggtaattcat
     1261 atatctctca tcgaaccgga cgggagaagc tggtccagga tagaacggat aaaagttgag
     1321 cggcctgatt tttatagaat aaccgcttac agaaaccttc tgagggagta cctcgatgat
     1381 gaaacttcct gggtcgatat cgtcgagagc cttaagctcc tcgaatcaac cagggaacgt
     1441 tatcctctcc tgctgaggag tatttgctcg ggcatcgcag ccatggcctt ctcctacgtg
     1501 tttggcggta acttacttga atcggtattt tctggcgtga taaccgctcc gatttttctt
     1561 ttgctttcgc tgatcccttc caacaggatg ttgatagatt ttctggcggg gtttatgact
     1621 acaattctgg cagttctctg cggttacttt tttgatctca attcttcgaa aataatcgtc
     1681 ggttcgataa tgccatatgt cccaggtatt cttataacga actcgattct cgagctggcc
     1741 aaccggaacc tcgtgtcggg cagttcgaag ttcaccgaag cgatactcat actcgtctct
     1801 ctggtgttcg gtgcaagttc ggcctttttc gtagcaggtg gtctggtatg attctcagag
     1861 tgctggcctc gctcattgcc ggagcgggat tcgctatcat cttcagtgtt cctgcgggca
     1921 taatacctct cgcttcgtgc gttgccgcgc tcggctggct ggtctatgaa ttcacctctg
     1981 gtttgctggg gctggctctt tcggtttttc tctcatcgtt gatcataggg attctttcca
     2041 ggttaatagc gacactgaag cactggcccc ttcagcccct tatagtggta ggaatagttc
     2101 ctcttgttcc cggttcttcg gctttctacg ccatgcagag tttcatgtca gaagatctct
     2161 tcagagcttt tgagtacagc tatcggacct tttccagtgc ctcaggcatt gtcatagggc
     2221 tggtggcctc atcaacagta ttcaatataa tagagaagat tattgagcta tcaaacaaaa
     2281 ataacaacat atagcggcct tgtagttaaa taatatgtat gattttataa taataatgaa
     2341 agcaactttt atctttagca tatatgcttt gaattaaatg acttatttac tggtattttt
     2401 gctgaagatt ccgtgattgg ggttttatac tttcgataca cgctgatttt attgtttatt
     2461 tgacccttga aatattgact ttccacttta aaaactggta tagtgaacac aagtactctt
     2521 ttttgctgtt tgccgaccag aatgggggtg ctgtcattga agagaagact aaccaagtgc
     2581 ccggtttgcg atggagatct caagataact gaatacacat gtccttcatg caaaataacc
     2641 ataagagggg acttctatca ggacgagata gtctcacttt ccgaagagct gcttgacttt
     2701 ctaaaagttt tcatcaagaa taggggaaat ctctccgaat tgcagaaaga gctgaacatc
     2761 tcgtatccca cggccagaag caagctggaa gagctggtca ccagcctggg ttatgaagtc
     2821 gcaggtcggc aggttcagga ggcttctaaa atcataaaga agctcgaaga tggtttgatc
     2881 gagcctgaag aggccgttaa gctactcaac cgtatgaagg gcaagcgttg aaaccacccc
     2941 tggctttcaa tacatttttc acctgtgcca tgttttccag aggagtcccg cggggtatct
     3001 cacaaccggc ggctataaag gttctgccgt cggatacatc cagacatttc tcgactgaat
     3061 cggcgacctt ttccacgctg cccttgaaca tgaccgatac cgggtcgaag ttaccgcata
     3121 tcgatatctt tcctcctccc agctccaccg ccgaagcgaa atcgaccatc cagtcaacat
     3181 cgattatatc ggcccctgtg tttatcatta aagaaagaat cggtgttgta tccccgcaga
     3241 tgtggagttt gacctttgcg cccgcctcgt gaacacgctt gatcagaagc ttttcgtagg
     3301 gaagtccgaa tttggagtac gatctaggtc cgataaccga acagatcgca tcgcctatgc
     3361 ctatgaagtc ggcccccgcc ctcacctgtt ctagggcgaa gatagtctcc tgctcgagac
     3421 agaattccag taactccacg acgaactccg gtctctcgaa gaaatcctcc ataattccga
     3481 ccggagttct gaggtcggca gccagggcga gcggcccttc gacccatccg agaaccgggt
     3541 attctgccga ggcgtgattg ttgtaaaaat cgatggcacc gattctgtcg ctcatcctcc
     3601 ttccggtgga gggttcgacc accttcagct tcaacggctc cgctgccgaa agcagtggaa
     3661 ggccgaagta agggatatcg tctcctggaa agatcacctc tcccccgagg tcgttaacct
     3721 ctctggcagg atcggaaatg gcgctgacca tatctattcc gaaatcctcg caacacctga
     3781 tgtttccctc gcaaaggacc ctgaaatcgg tggcgtactc tctgtaggtc ttgccgatat
     3841 atttcgccgc gaaggccatt attatgttca aattcggtac ctgatccacg cttcgtcctt
     3901 cgattctcgc aaagactctc tccctagaag tcaaaaccgc acctcctaaa aataagagac
     3961 ttgaatcctc tcccatatag attgtaatat cttttctgag agaatgtaga ataaaattaa
     4021 agtggataat catttcggtt ctggaagggg tgttttcgtt tgcgggttgt gaaggcgata
     4081 ttcaccgtgc tcttcgtttt gctcatcgcg gtcatagtgc atgcgatctt cagtgttaca
     4141 atggccagaa tatacctggg acgcatggac caggatggcg aatacataac cgttggagaa
     4201 gaaaagctgt atatcgaagt ctccggagcg ggcgatccga taatattaat acacggcttt
     4261 ttgggctctt acctcgattt cgatgctata gtggatgggc tttcgacctt cagaaaggtc
     4321 tatgtgatcg atctgccggg gtttggcctt tccagagctt ctctggaggg tgattacagc
     4381 aaaaagggtt atgccgatct tgtgagcggc ttgatgcata ttctcaattt aaatctggcc
     4441 gatgtcctgg gtcactccat gggcggggaa gttgctctta atcttgctta ttatcacccg
     4501 gagagagtga agagtctcat actggtcgat tcgatgggat actcgaagat gaatttcttg
     4561 ccgtctcttg ttgaggataa cgagtttctg aaccatgtgc tcatgaggta tggctttcag
     4621 acctatctgg ttcagcgttt tctttataga cagaaactag gggacgtttc gaagttcaac
     4681 cgtgaatcct tccatttgag cttttcgctg ataaatcata tctccccggc ctttctttac
     4741 aggttcaatt tgcaggacga tagcggagcg ctttcaggga agattcgaga tataaagtcc
     4801 agaactctga tcgtctgggg agagaaagac agaatagcgc cggttacaga cggcgaaagg
     4861 ttcaaaatcg atatcccggg cagtgagcta gaaattataa cgggtgtcgg tcactcgcca
     4921 atgctggaaa atccgtctct ctttctggag aagttgaagc ttttcctgaa ataaacacga
     4981 gatcattaca gtggccgtga aacggcagga ggctttcatg ggtaaattcg cccgaccgga
     5041 agttgttgtc agcaggtgcc tgggatttga aagctgcagg tacaacgctc agaccgtacc
     5101 tagcaatttc gtatcgactc tgggtaattt cgtcaattac acaactgtct gccccgaagt
     5161 agatataggg ctcggcgtcc cgagggcacc catcaggctg atagccctgg atagtgtggt
     5221 caggttgatt cagccttcca caggattgga tgtaacggag aagatgaacg cctatgcctc
     5281 ttcccttctc tcttctctcg aaaatgtcga tggattcatt ctgaaggctt tatctccgtc
     5341 ctgcggaatc aaggacgtca aggtttaccc gggtgcggga aaggttatgg ctacgggaaa
     5401 agacagcggt attttcgggg gagaggttgt aacccgcttc ggccaccttc cggtagaaga
     5461 cgaggggaga ctcaccaact tcgttttgag agaacacttc ctcaccacgc tcttcaccgt
     5521 gacccgattc agagagctca atctgaaccg ttctatcaaa ggcctcgtgg attttcagag
     5581 cgataacaag ctactcttca tgtcctacaa tcaaaacgag atgagagcta tggggagaat
     5641 agccggaaat ctagaaaaga aagatctgaa cg
//
LOCUS       DAOOLY010000159         5665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_17102_length_5665_cov_4.018004, whole genome shotgun sequence.
ACCESSION   DAOOLY010000159 DAOOLY010000000
VERSION     DAOOLY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17102_length_5665_cov_4.018004"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLD15_08295"
     CDS             complement(<1..849)
                     /locus_tag="PLD15_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPB63947.1"
                     /translation="MSILLKNGTIYPITSKPFAGDILVSKGKIESIGVDLKCEGAEVI
                     DASGKYIFPGFIDAHSHIGLFEEGVGGYYQDGNEMTDPLTPDVSVVDAFSPQDAAIKR
                     ALSGGVTTVMVVPGSANPIGGQGAIFKFKNTMIVDDMVVRAPAGLKMATGENPKRVYG
                     EGFKKTPGTRLGTAAVIRGYFQKVQGYMEKKAIVARDGKSFTEIDPKLEIGEKVLRKE
                     IPARIHAHRLDDILTAVRLSKEFDFKLVIEHATEGYKIVDYLKENNVPVVIGPIFGFR
                     TKLEDRK"
     gene            949..1377
                     /locus_tag="PLD15_08300"
     CDS             949..1377
                     /locus_tag="PLD15_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPB63948.1"
                     /translation="MLEDSVANHYCATDVERNCSEALIYGTNDEFLLGLPVEALRTMG
                     PFGGGMRIESICGAITGALAVIGVLYNRDNPTERDRMKEIAERFLSEFKNRYGCLECL
                     CLKDKYRDEKKKCEPVVRMSARLLEEIIKVEESRRQFEKE"
     gene            1677..2756
                     /gene="ychF"
                     /locus_tag="PLD15_08305"
     CDS             1677..2756
                     /gene="ychF"
                     /locus_tag="PLD15_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPB63949.1"
                     /translation="MEIGIFGLPLSGKSTLFSLLTGVEPHATHKNEASTAVAKVYDNR
                     VEELSKIFNPKKTIYATVTFVDIPGYDLSSGQKEKSRILQFIQNSDAIVAVVRAFKEA
                     SVPWPAGASSPSKQLETIETELLVRDMEVVENRISRLDEARKKRKLSKEEERELELLG
                     EIEKGFEENRFVSVMGFSEEDLKLLGSLALFTAKPIIVAVNVDEDQFTSKEYPEKTAI
                     TARVAESGFAYIEISGKIEVELNELPEEDRGMFMEELGISESGIQRLSRVAYEHLGLI
                     SFLTVGEDEVRAWTIRKDTPAKAAAGKIHTDLEKNFIRAEVIPYEEFMTVKSMHEAKN
                     KGLIKLVGKEEIVRDGDIFHVRANA"
     gene            2776..3534
                     /locus_tag="PLD15_08310"
     CDS             2776..3534
                     /locus_tag="PLD15_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPB63950.1"
                     /translation="MDTHAHVSFPQFDHDRDRIKAQLESGELSMLIEVGTTVEDSLRA
                     MEVFKGVKNVLLSVGVHPHESDGLDDRGLETLKSLLKDRQVVAVGEIGLDFYRDLSPR
                     EVQKSVFLKQLEIAFSFSLPVVVHVRDAYEEVYEILRRFKGLKGVIHAFSGGPAMAGR
                     FLELGYYLGIGGPITYRKNDGLREIVRLTPIEKLISETDCPYLPPVPFRGKRNEPLYV
                     RYVVEEIARQKEMDIYTCSKQLMYNAKELFTPDL"
     gene            3548..3898
                     /locus_tag="PLD15_08315"
     CDS             3548..3898
                     /locus_tag="PLD15_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPB63951.1"
                     /translation="MGGFEEFKSFREKMNVRILETGSINTKRFFSLDGAVYKDGALDT
                     ITKELMGLVASTVLRCDDCISYHIIQCKSIGITRQEIMEALDIALVVGGSIVIPHLRR
                     AVALLDELEEKGAG"
     gene            3898..>5665
                     /locus_tag="PLD15_08320"
     CDS             3898..>5665
                     /locus_tag="PLD15_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPB63952.1"
                     /translation="MAGRLFLFDGTAIFYRGYYGIDVSLTNSKGEPTNALFGLARMLS
                     KFTREHMDEGDYALFAFDRKELTHRHKMFEAYKATREAMPEALVAQLKDVPGLVEAFG
                     LKYFSLAGLEADDIIATVTKKYRDSVEKVFIISGDKDILQLVDDKVNVLRFVTGLTDL
                     ELYDRQAVIDKFELEPERLHSLLTLAGDQSDNIPGVSGIGIKTAQKLLKEYGSIEGIY
                     ENIRQLSQSLKQKLIDGKRSMELSRELVKLLTDEKLDLTLDELEYHGYIKEYLREILL
                     RLEFSSMIDEYGLTSEAEANLSDIDGYTLIASDEELRKLLEKVRSSPVVSLDLETSSL
                     DPHSARIVGVSISIEEKSGYYIPVGHDTQRWQANCRWLLSQLREILENPGTRIVGQNL
                     KFDYSILALNEIHPVIPEFDTMIAAYLLSPDSRRFNLDELALKFLGYKTVTFEDLMKR
                     NQLGDDFTKVPLEEAARYSVEDADISLQLSSVLRKKIYEQKLEKVFREIELSLIPVLS
                     DLELKGVYFDIQSLEKLSGDYGRKIDFILGELFELTGEQFNPNSPAQVGRVLFDKLGL
                     APSKKTRGGSFSTSADALEELSE"
BASE COUNT         1597 a         1248 c         1429 g         1391 t
ORIGIN      
        1 cttccgatct tctagttttg ttctgaagcc gaagattggt ccgataacca ccggtacgtt
       61 gttttccttt aaataatcga caattttata cccttcggtg gcgtgctcaa tcacgagctt
      121 gaaatcgaat tcttttgaga gccttacagc cgtgagaatg tcgtctagcc tgtgggcgtg
      181 aattctagcg gggatctcct tgcgtaacac tttttcgccg atttcgagtt tcggatctat
      241 ctccgtgaaa gacttcccat ctctggcgac tatagccttt ttttccatgt aaccttgcac
      301 cttctggaag tatcccctta taaccgcagc cgttccaaga cgcgttccag gggtcttttt
      361 aaagccttca ccatagactc tcttcggatt ttccccggtc gccattttca aacctgctgg
      421 cgctctgacg accatgtcat cgactatcat ggtgttttta aatttgaata ttgctccctg
      481 gccaccgata gggttggcgc ttcccggaac caccataacg gttgtaaccc cgcccgaaag
      541 agctctcttt atcgctgcat cctgcggact gaaggcgtca accacagaga catccggtgt
      601 cagaggatcg gtcatctcgt tcccgtcctg gtagtatcct cctacgccct cttcgaaaag
      661 ccctatatgt gagtgagcgt ctatgaaccc ggggaagata tacttaccgg atgcatctat
      721 tacttcggca ccttcacact tgaggtctac tccgattgac tcgatctttc ccttgctaac
      781 aagtatgtct cccgcgaacg gtttcgaggt gataggatag attgttccgt ttttcagaag
      841 tatcgacatg gtaaccccct ttgaaaatcg aattattgaa tgtattttat caaaaatggt
      901 tcttattaca aactataaga acgacttcaa aagaacggga gagaaaatat gcttgaagac
      961 agtgtagcga accattactg tgcaacagat gttgagagga actgctctga ggctttgatc
     1021 tatggtacga acgatgagtt cctacttggc cttccagtag aagctttgag gacaatgggt
     1081 ccttttggcg gggggatgag gatcgaaagt atttgcgggg caataacagg tgctctggcg
     1141 gtgataggtg ttctctataa cagagacaat ccaaccgaga gagacagaat gaaggaaatc
     1201 gcggagcgat tcctgagcga gttcaaaaac aggtacggct gtctggaatg tttatgtctg
     1261 aaagataaat acagagacga gaagaaaaaa tgcgagccag tagtcaggat gtcggcaaga
     1321 cttctcgagg agataataaa agtagaagag agcagaagac aatttgaaaa ggaataaaaa
     1381 cccttccggt atgtaagttc ggatcgttca tgatcaaaac tttgatcgta cgatgaaagt
     1441 cttatacaaa tgaatgaatt taataacagc ccagattcaa taccatgagc aattctgata
     1501 atatagatat gaaaagcgaa acaaatacga tgaaggaaga aattatatcc cactctggtg
     1561 gagcttatgg aacagaggtt ttcgcaggaa gtgtcgatgt ttcacgaaaa gtttcattct
     1621 gaagtattgc atggtaattc cgacaacctc tggttcacgc acaaggaggc gatgaggtgg
     1681 aaatagggat tttcggtctg ccgctgagcg gcaaaagtac tcttttttca ctgctgaccg
     1741 gggttgaacc gcacgcgact cacaaaaacg aagccagcac agcagttgca aaggtttacg
     1801 ataacagggt cgaagagctt tctaagattt tcaacccaaa gaaaaccatc tatgccaccg
     1861 tcacttttgt agatatcccc ggttacgacc tctcttctgg ccagaaagaa aagagcagga
     1921 tactccagtt cattcagaat tccgatgcca tagtagccgt agttagagcc ttcaaagagg
     1981 cttccgttcc ctggccagcc ggcgcaagca gtccttcgaa gcaattggaa acaatagaga
     2041 ctgagctact ggttcgtgat atggaagtag tggagaaccg tatctcccgg ctggacgaag
     2101 ctagaaagaa aagaaagcta tcaaaggagg aagagcgcga gctggaactt cttggagaga
     2161 tagaaaaagg ttttgaagag aacaggtttg tttcagttat gggcttttcc gaagaggatc
     2221 ttaagcttct gggttcgctg gcgctcttca ccgcaaagcc gatcatcgtt gccgtgaacg
     2281 tggatgaaga tcaattcaca tcgaaagagt atccggaaaa gacagcgatc acagcccgtg
     2341 tcgcagaaag tggattcgcc tatatagaga tctctggaaa aattgaagtc gagctcaacg
     2401 agttgccaga agaggataga ggcatgttca tggaggagct aggcatctct gaaagcggca
     2461 tacaacgtct ctcaagagtt gcgtacgaac atcttgggtt aatctcattt ctgaccgtcg
     2521 gcgaggatga ggtcagggca tggacgataa gaaaagatac gccggccaaa gcggctgccg
     2581 ggaagattca tacagatctc gagaagaatt tcatcagggc cgaagtgata ccttacgaag
     2641 agttcatgac tgtgaaaagt atgcacgagg ccaagaacaa aggattgata aagctcgtgg
     2701 gaaaggaaga gatcgtgagg gacggtgata tcttccatgt cagggcaaac gcctgataat
     2761 atccatcttg aactggtgga tactcacgcg catgtttcct ttccacagtt cgatcacgat
     2821 agggatagaa ttaaagccca gctcgagagc ggcgaactgt ctatgctgat agaagtcgga
     2881 acaaccgttg aagattctct gagggcaatg gaggtcttca aaggtgtcaa gaacgtctta
     2941 ttatcagtcg gggtccatcc ccacgagagt gatggtctcg atgaccgggg tttggaaact
     3001 ctgaaatcgt tgttgaaaga tagacaagtc gtggcagtgg gagaaatcgg cctggacttc
     3061 tacagagatc tttcgccaag agaagtgcaa aagagtgttt tcttgaagca acttgagatc
     3121 gctttctctt tttcgctgcc ggtcgtggta catgtgagag atgcatacga agaagtttat
     3181 gaaatactcc gtcgattcaa gggactaaaa ggagtgatac atgccttttc gggaggtccg
     3241 gcgatggctg gcagatttct cgagctgggt tactaccttg gaataggtgg accgatcaca
     3301 tacagaaaga acgacgggtt gagagagatc gttaggctga cgcctattga gaagcttata
     3361 agcgagacgg actgtccata tcttcccccg gtcccgttca ggggaaagag gaacgaacca
     3421 ctttacgtaa gatacgttgt cgaagagata gcccgccaga aagagatgga tatctacacc
     3481 tgctcgaaac agcttatgta taacgcaaaa gaactgttca ctcctgacct ctgaaggagg
     3541 tgtgatcttg ggaggatttg aagaattcaa gagttttcga gaaaagatga atgtaagaat
     3601 actggagacg ggaagtatca atacgaaaag gttcttctcc ctggacggtg cagtttataa
     3661 agatggcgct ctcgatacca taacgaagga gctgatgggt ctggtagcct cgacggtgct
     3721 aagatgcgat gactgcattt cctaccacat aattcagtgt aaatcaattg gaataacccg
     3781 acaggaaatt atggaagccc ttgacattgc tcttgtcgtt ggcggatcga tagtgatacc
     3841 ccacctccgg cgcgccgtag cgcttctcga cgaactcgaa gagaaaggag ccgggtgatg
     3901 gccggcaggt tatttctctt cgacgggacc gccatcttct acagaggata ctacggtatc
     3961 gacgtttccc ttaccaattc aaaaggtgaa ccgaccaacg ccctcttcgg tctggcccgg
     4021 atgctttcaa aattcacccg tgaacatatg gacgaaggtg attacgcact atttgctttc
     4081 gatagaaagg aattgaccca cagacacaaa atgttcgaag cctacaaagc gaccagagaa
     4141 gcgatgccgg aggctcttgt tgcacagttg aaggatgttc ccggtctagt ggaagctttc
     4201 ggattgaaat acttctctct cgccggactc gaagccgacg atataatcgc cacggttaca
     4261 aagaaataca gagatagcgt cgaaaaggtc ttcataattt caggtgacaa ggatatcctt
     4321 caactggtcg atgacaaagt taacgtcctg cgtttcgtca caggccttac cgatcttgag
     4381 ctatacgaca ggcaggcagt gatcgataaa ttcgagctgg aaccggagcg gcttcattct
     4441 cttcttactc ttgccggtga ccagtccgat aatattccag gtgtttctgg aatcggtata
     4501 aagacagccc agaagttgct caaagagtac gggagtatag aagggatcta cgaaaacata
     4561 aggcagcttt cgcagagtct caagcagaag ctgattgacg gcaagagatc gatggaactt
     4621 tccagagaac tggtgaagct cctgaccgat gaaaaactcg acctcacact tgatgagctg
     4681 gagtatcacg gctatataaa ggagtacctt agggaaatac tgctaaggct ggagttctct
     4741 tccatgatag atgaatacgg cctaactagc gaggctgaag ccaatctgtc cgacatagat
     4801 ggatatactt tgatcgccag cgatgaagag ttgcgaaagc tcctggaaaa ggtcaggagc
     4861 agtccggtcg tctcactgga tcttgagacc tcctctctcg atcctcacag tgccagaatt
     4921 gtcggtgttt cgatatctat cgaggaaaag agcggatact acatacctgt cgggcacgac
     4981 actcagaggt ggcaggccaa ttgcagatgg ctgctttcac agctgcggga gatactcgag
     5041 aatcccggta ccaggatcgt cgggcagaat ctgaaattcg attactcaat ccttgccctg
     5101 aacgaaatcc atcctgtaat acccgaattc gacaccatga ttgcggccta tctactctct
     5161 cccgattcga gacgcttcaa cctggacgag ctcgcgttga agtttctcgg ctacaagacg
     5221 gttaccttcg aagatctaat gaaacgcaat caactgggcg atgacttcac aaaggtacct
     5281 ctagaagaag ctgccaggta ctcggtcgaa gatgcagata tatctttgca gctctcttcc
     5341 gttctcagga aaaagatata tgagcagaag ctagagaagg ttttcaggga gatagaactg
     5401 tccttgatcc ccgttctatc agatttagag ctgaaaggtg tttatttcga cattcaatca
     5461 ctggagaaac tctcaggcga ttacggcaga aagatagatt tcatactggg cgagctcttc
     5521 gaactaacgg gtgagcaatt caaccccaac tcaccggcgc aggtgggaag agttctcttc
     5581 gataaacttg gcctggctcc ttccaagaag accagggggg gttctttctc gacgagtgca
     5641 gatgcccttg aagaactctc cgaag
//
LOCUS       DAOOLY010000160         5649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_17162_length_5649_cov_4.740972, whole genome shotgun sequence.
ACCESSION   DAOOLY010000160 DAOOLY010000000
VERSION     DAOOLY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17162_length_5649_cov_4.740972"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLD15_08325"
     CDS             <1..363
                     /locus_tag="PLD15_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63953.1"
                     /translation="LKQFFDTVPREFSDAAKLDGANEWKIFLKVYLPLSKPALATSAL
                     FIFIWTWTDFLNPLIYLNDDRMYTLSIGLQQLSTSRTTAWPQLMGGSVLMTLPIIVLF
                     FLAQKTFIQGIQVGGIKS"
     gene            393..1424
                     /locus_tag="PLD15_08330"
     CDS             393..1424
                     /locus_tag="PLD15_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPB63954.1"
                     /translation="MFKKGILLLLALMVVSFMFAGEFNVSAGKTYTLSPEPNPNYADT
                     GTKLTDGSFNFSWGDMVGWENPVANPTIVVDLGAVFEEVSYVALKIMYSAPSAVNLPE
                     YFIVSISEDGELYEDLGMGITYAEAPVPNDTIATLYWASDEFVGYGRYVKIEVVPGGE
                     AWTMVAEVIVGDGAFPEELGAVVGGTVDLTEAATVSVGKPYMLIPLSSDAYADTDSIE
                     VTDGFARYSWADMIGFDNPAINPTIIIDLLEPTSIVRVSSHFMRSFASAVNLPNSLLI
                     GVSDDGMEFRTVGLATITNPYPAQNEFINEIYWVAGEPVVARYVKIEIRPRGNAWTML
                     AEATVWAVK"
     gene            1429..2922
                     /locus_tag="PLD15_08335"
     CDS             1429..2922
                     /locus_tag="PLD15_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HPB63955.1"
                     /translation="MFDKRVVFLPVDERFCTRDYFLLLARAANINVVTPPKSYLGAKK
                     TPPDIRRLLKWLEETVVPGDYLVLSIDMLVHGGLIPSRMSIETLDTLKERLGLLEKLK
                     DRDVKIYATTTVTRTPFYNSAEEEPDYWQYFGLDVYDLSRLLARSFRGERVHRSVIEK
                     MGKIPVHFVKDYLVRRVRNRKLVSLVIELVERGVLDFLNLVLDDNSKESISLAESEIH
                     QARVDSLGIGPKVSIHAGADEATLSLLSRVLSESLGVVPTFEVRYSSPEYKELVPPYE
                     GSPLHRGIRNHIEAAGGRVESEGDIVLLTNNPEIGLDSPQQPADPADPGPYERFFADL
                     KAAGRKIKGIADVKYTNGADNYLVKGLLKGELDWLETNYSAWNTAGNTLGTVCAHSIV
                     QFLGQKGLLNLERSKIKELQAIFFLEHWAFQSNVRQKLLVEAEKKGARPWTVIPVESW
                     AEEFVRKELTAFVPPVRKALGIDFEIERLFFPWHRSFELGLCVATKR"
     gene            2946..3662
                     /locus_tag="PLD15_08340"
     CDS             2946..3662
                     /locus_tag="PLD15_08340"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HPB63956.1"
                     /translation="MDFGKIEKKIIVSCQALEDEPLHGRGVMVLMAKAAIMGGASAIR
                     ANGAQDIEDIKAAFDIPLIGLNKRNIEGYRVYITPTSEDALAVLAAGADVVAIDCTLR
                     KRPEPLDSIFFNIRREFPDRLIMADISDLKDAKAVLDLRPDFLATTLSGYTDDSSDKP
                     KPDIELVRVLSREFDIPVVAEGNYWEPEQVVRAFEAGAFSVTVGSVITRPQLITRRFT
                     SYLEEWKKDRLMSRAGGRSH"
     gene            3659..4933
                     /locus_tag="PLD15_08345"
     CDS             3659..4933
                     /locus_tag="PLD15_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB63957.1"
                     /translation="MIRLKKDVVIVGAGISGVHAAIAAGKAGLDVLLVEREGSVGGIS
                     TLGLCNPFMRFWLDSGSLVGGIFEEILKDLARRGGLLANSFDSEIMKIIYFEKLKSAG
                     VSLVIHSIPIEVERCGRLIKSVKFFSSQGSAFEVEARYFVDATGDATLSYMAGAETFQ
                     GDESGSNQAMTLMFTVSGIDYARVREDIKSNGENFFAWVKPDFEIMSVAGYFEEIERA
                     RKAGYDKLHNYFFFIELPGSGRASVNTTHSFDKSAVDPFELSEAVTDCMAQTDQLITF
                     AREYVRGFEKSGIEKIATLMGVRESRRVRGLYVFSGEDVKAHRKFPDGVVRATYGIDV
                     HNRSTQRVSEEERKAIPVYNDYYEIPLRSLISADLDNLAIIGRCFSSDFSGQSAARVM
                     PTVAGMGQAIGRVLYQAISRGESLSEIAIDGR"
     gene            complement(4941..5459)
                     /locus_tag="PLD15_08350"
     CDS             complement(4941..5459)
                     /locus_tag="PLD15_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB63958.1"
                     /translation="MTEAFGFSLPTAILLLNIGIVSFIGIICLIGYFKTRGWQRIFST
                     TLTGVLLVVFIFLFIVFPLSNNIRVDDNGVILNALPFGNKIIALNSAEIAGIIDWQES
                     RDFEPTLKTNGASTGSYKVGWFKLRNGSKAFLMTARSKVFVFKSEDIYYLISPDELEK
                     FSQAVFSRSQSL"
BASE COUNT         1383 a         1438 c         1610 g         1218 t
ORIGIN      
        1 ctgaaacagt ttttcgacac cgtaccgaga gagttttccg acgcggcgaa gctcgacggc
       61 gccaacgagt ggaagatctt cctgaaggtt tatcttccac tgtccaaacc ggctctggcg
      121 acttcggcgc tgttcatatt catatggacc tggactgact ttctgaatcc gttgatctat
      181 ctgaacgacg atcgaatgta caccctctcg atcggtctcc agcaactgtc gacatcgcgt
      241 acgacggcct ggccgcaact catggggggt tccgtactga tgacccttcc cataatcgtt
      301 cttttctttc tggcacagaa gacattcatt caaggaatac aggttggagg tatcaaatca
      361 tagagcacct tgaaaaagga ggaggaataa aaatgttcaa aaagggtata ttgttacttc
      421 tcgcgttaat ggtggtttct ttcatgttcg ccggtgagtt caacgtgtcg gccggcaaga
      481 cctatacgtt gagtcctgag ccgaatccca attacgcgga tactggaact aaactcaccg
      541 atggcagctt caacttcagc tggggagaca tggtgggctg ggagaacccc gtcgccaacc
      601 ctacgatcgt tgtcgatctc ggcgctgtct tcgaagaggt gtcttacgtc gctctgaaga
      661 tcatgtactc ggccccttcg gccgtcaatc tgccggagta tttcatagtg tccatctcgg
      721 aagacggcga actatacgag gatctgggca tggggataac atacgcagag gctccggttc
      781 ccaacgacac tatcgcaacg ctctactggg ctagcgacga gttcgtcggc tacggaagat
      841 acgtgaagat cgaggtagtt cccggaggag aggcctggac tatggtcgcc gaggttatcg
      901 tgggagacgg ggccttccca gaagagctcg gagccgtcgt cgggggaacc gtggatctga
      961 cggaggccgc gactgtttct gtcggcaagc cgtacatgct cataccgctt tcctcggacg
     1021 catatgcgga caccgattcg atagaggtaa ccgacggctt tgcgaggtac tcctgggccg
     1081 acatgatcgg cttcgacaac ccggcgatca atccgacgat aataatagat cttctcgaac
     1141 ccacttcgat agtcagggtt tccagccact tcatgcgctc gttcgcctcg gcggtcaacc
     1201 tgcccaacag tcttttgata ggcgtaagcg acgatggaat ggaattcagg accgtcggtc
     1261 ttgccacaat taccaatccc tatcctgcgc agaacgaatt cataaacgag atatactggg
     1321 tggccggtga acccgtagtg gcgagatatg tgaagatcga gatcagaccc aggggcaacg
     1381 cgtggaccat gctggccgag gccacagtct gggcagtgaa gtaaagatat gttcgacaaa
     1441 agagtcgtat ttctccctgt agacgagcgc ttctgcacga gagactattt cttgctactg
     1501 gcaagagcgg cgaatataaa cgtcgtgact ccgccaaaat cctatctggg ggcaaagaaa
     1561 acgcccccgg atattcggcg actgctgaaa tggctggagg agaccgtcgt accgggcgat
     1621 tatctcgtcc tttcgataga catgctcgtg cacggcggct tgataccctc gcgaatgagc
     1681 atagagactc tcgatactct caaagaaagg ctgggactgc tcgagaagtt gaaggatcgc
     1741 gacgtgaaga tctacgcgac gacgaccgtt accagaacgc ccttttacaa ctcggccgaa
     1801 gaggaacccg attactggca gtacttcggc ctggatgtgt acgacctttc caggctgctg
     1861 gccaggagct tcaggggcga gagagtccac cggtctgtga tcgagaagat gggaaagatt
     1921 ccggtccatt tcgtgaagga ttatctcgtg agacgcgtca gaaatcgcaa actggtttca
     1981 ctcgtgatag aactcgtcga gaggggcgtg ctggactttt tgaacctggt tctcgacgat
     2041 aacagcaagg agagcatctc tctggccgaa agcgagatcc accaggccag ggtagattct
     2101 ctgggaatcg gcccgaaggt ttccatacac gcaggcgccg acgaggccac gctcagcctt
     2161 ctatcgagag ttttgtcgga gtcgctcggg gtcgttccga cttttgaagt gcgctactcc
     2221 tctcccgaat acaaagagct ggtgccgcct tacgaaggct ctcccctgca ccggggcata
     2281 cgcaaccaca tcgaagcggc cgggggaagg gtggagagcg agggtgacat cgtacttctg
     2341 accaacaacc ccgaaatagg cctagacagc cctcaacagc cggccgatcc ggccgatccc
     2401 ggtccctacg agagattttt cgccgatctt aaggccgccg gtagaaagat aaagggtata
     2461 gccgacgtga agtacaccaa cggcgcggac aactatctgg taaaggggct tctgaaaggc
     2521 gaactggact ggctggaaac gaactactcg gcctggaaca cggccggaaa cacgctcggt
     2581 accgtgtgcg cccactcgat agtgcagttt ctcggccaga aggggttgct caacctcgag
     2641 cggtcgaaga tcaaggaact ccaggcgatc ttcttcctcg agcactgggc ctttcagtcc
     2701 aacgtgaggc agaagctgct ggtcgaggcc gaaaagaagg gagcccggcc gtggacggtt
     2761 atccccgtgg aatcgtgggc cgaggaattc gtcaggaagg aactgacggc ttttgttcca
     2821 cccgttcgca aggcgctggg aatagacttc gaaatcgaga ggctcttctt cccctggcac
     2881 aggtccttcg agctgggcct ctgcgtagcg acgaagcgtt gaactctgag gtgatagtat
     2941 tgggtatgga ttttggaaaa atcgaaaaga agataatagt ctcctgccag gctttagagg
     3001 acgaacctct ccacggccgc ggggtcatgg tgttgatggc gaaggccgcc atcatgggag
     3061 gggccagcgc cataagggcc aacggcgcgc aggacattga agatataaag gccgccttcg
     3121 acatacctct tattgggctg aacaagagaa acatagaggg ttaccgcgtc tatataaccc
     3181 ccacttcaga ggatgccctc gcagtcctgg cggccggcgc cgacgtggtg gccatagact
     3241 gtaccctgag gaagaggccc gaacctctgg actcgatatt tttcaatatc aggagggagt
     3301 tccccgacag actgataatg gccgacatct ccgacttgaa agacgcgaaa gccgtgctgg
     3361 acctgaggcc tgattttctc gcgaccacgc tttcgggata caccgacgac agcagcgaca
     3421 aacctaaacc cgacatagag ctggttagag tgctgtccag agagttcgac attcccgtgg
     3481 tggccgaggg caattactgg gaaccggagc aggtcgtcag agccttcgag gccggggcct
     3541 tctccgttac cgtggggagc gtcataacca gaccccagct gataacgagg agattcacga
     3601 gctacctcga agagtggaag aaggaccggc tcatgtcccg ggcgggaggt cgctcacatt
     3661 gatcaggttg aagaaggacg tggtgatagt cggggccggc atttccggcg tgcacgcggc
     3721 gatagcggcc gggaaggccg gtctagacgt tcttctcgtc gagcgcgagg gctctgtcgg
     3781 aggcatatcc accctcggtc tctgcaaccc cttcatgagg ttttggctgg actcggggag
     3841 ccttgtggga ggaatcttcg aggagatact gaaggatctt gccagacgcg gagggctgct
     3901 cgcgaactcc ttcgattcgg agataatgaa gataatatat tttgaaaaac tgaaaagcgc
     3961 cggtgtctct ctggttatac attctattcc gatcgaagtc gaaagatgcg gccgactgat
     4021 aaaatccgtc aagtttttct cgtctcaggg gagcgccttc gaggtagagg ccaggtattt
     4081 cgtggatgcg accggggacg ccacgctctc atacatggcc ggggccgaaa ccttccaggg
     4141 cgatgaaagt ggctcgaacc aggccatgac tttgatgttc acggtatcgg ggatcgatta
     4201 cgcccgggtc cgggaagata taaaaagcaa cggggaaaac ttcttcgcct gggtgaagcc
     4261 ggacttcgaa atcatgtccg tggccggcta tttcgaagag attgagagag ccaggaaggc
     4321 cgggtacgac aaattgcaca attacttctt cttcatagag ctccccggaa gcggaagggc
     4381 ctcagtcaac accacccatt ccttcgataa aagcgcggtc gatcctttcg aactgtccga
     4441 ggccgtcacc gactgcatgg cccagacgga ccagctgata acctttgcca gagagtacgt
     4501 caggggcttt gaaaaatcgg gaatagagaa aatagcgacg ctcatgggcg ttagggaaag
     4561 cagaagggtg agagggcttt acgttttcag cggcgaggat gtgaaggccc acaggaagtt
     4621 ccctgacggc gtcgtgaggg ccacttacgg catagacgtc cacaacaggt caacacagcg
     4681 ggtgagcgag gaagagagaa aggccatccc cgtctacaac gattactacg aaataccgct
     4741 gagatctctg ataagcgccg atttagacaa tctcgcaatc atcgggcgct gtttttcgag
     4801 cgacttttct ggtcagagcg ccgcaagagt catgccgacc gttgccggca tgggccaggc
     4861 gataggccgg gtgttgtatc aggcgatctc ccgtggagag agcctctctg aaatcgccat
     4921 cgacggccgc tgaccagttc ttacagagat tgagatctgg aaaaaacggc ctggctgaac
     4981 ttctccagtt cgtccggaga aatcaggtaa tatatatctt cggatttgaa aacgaagacc
     5041 ttcgaccggg ctgtcataag gaaagcctta gaaccgtttc tcagcttgaa ccagccaacc
     5101 ttgtaactgc cggtagatgc gccgttcgtc ttcagagtcg gttcgaaatc gcgactttcc
     5161 tgccagtcga tgattccggc tatttcagca gaattgagag cgataatttt gtttccgaaa
     5221 ggtaaggcgt tcaggatgac tccgttgtcg tcaactctga tattgttgct caagggaaat
     5281 actatgaaga gaaagatgaa gacaaccagc agtacaccgg tcaatgtggt ggaaaagatc
     5341 ctctgccatc ccctggtctt gaaatagcca atgaggcata ttatgcctat gaatgaaact
     5401 atacctatgt tcaacaacag aatcgctgta ggtaaagaaa agccaaaagc ttcagtcatt
     5461 tccttctcct taaaaagaat ctctatatat tatgccacag acggcggatc cataagaaaa
     5521 atgatgtatg atgatcttgc agacgggtct ttcgctcgtc tgcggacagg caccgaacta
     5581 cttcagaaga gttctccaaa gaggtgtgtg aatgcgtgct ctcggtatag atatcggtgg
     5641 aacgatgat
//
LOCUS       DAOOLY010000161         5560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_17529_length_5560_cov_4.219982, whole genome shotgun sequence.
ACCESSION   DAOOLY010000161 DAOOLY010000000
VERSION     DAOOLY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17529_length_5560_cov_4.219982"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLD15_08355"
     CDS             <1..741
                     /locus_tag="PLD15_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB63959.1"
                     /translation="IAAMEALIPITPAGLTPYYENPAWTWIWFHLLWQHGGDLLTDDF
                     KKPAFAKAGIEACKLMLTMQEKGILPMQVADPGVSFQSGDSTVLITGIWTIQPWMEQL
                     GEAFGYAVSPQLGTTKAVFGGSHVLAMPKVMVEDPKVLDAATTFMKYLWDNAIDWYAA
                     GQTPARISISQSQELKERLPHIYVVSQESEYVKQFQMFPLISEIEAEIAVYLDEILVL
                     HSISPEEGMKEAEFAVQEILDDYWSRAR"
     gene            833..1687
                     /locus_tag="PLD15_08360"
     CDS             833..1687
                     /locus_tag="PLD15_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63960.1"
                     /translation="MFLLPHLFFFVLFVAIPLVFGMVISFFNWSLLSDNVFVGASNYV
                     RTWNDSRFWPVVQNTVIFAIVSVPLTIIVAILFAHILNKKRFGQLWLLIAFVSPAFFG
                     SVGILSSWKWIFASFPSGLANYYLNKTGFINGAVSWFGSTGVAWGVIILVTIWWIVGF
                     SILLYMGALQRIPPEQYESAKLDGAGPWKRFLYITLPWIRSVLFFDVVRQVILAFGLF
                     DQAYILSVGGPAGTTRTMVYYLYLVGFERQDFGRAASISWFIFAIVLIFAVIQLVLVT
                     KSIRSTEG"
     gene            1692..2570
                     /locus_tag="PLD15_08365"
     CDS             1692..2570
                     /locus_tag="PLD15_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63961.1"
                     /translation="MTSTVAINKKKLSKRITAVVVTILLWAFAAFWFAPIFWMLSTSL
                     KSTAVAVIQTPPEWIPENPTLENYKIIFAPSGGLSLIRATLNSVIVAVGSTLATLALS
                     IPAAYALSRLRFRGRMLVFWIYVAVLAFPGVLFLVPHFFIIQGMGLTNSYLALILPGL
                     GGTFGVFLFRQYMLDIPGELEDAAWIDGCSKFRFLISIVIPYVKPAMLVLGLMTFLGS
                     WNSFLWPLLILSKSDKFTLPIALIRFSAGWGDPYRGIGPMMAGAFFSVAPSLIIFVVF
                     HRYLMKGISLGSLGKE"
     gene            2696..3796
                     /locus_tag="PLD15_08370"
     CDS             2696..3796
                     /locus_tag="PLD15_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPB63962.1"
                     /translation="MPEVDTSLFEERIENDRKIPISISIVDEQGRPVSKAFLTAKQLS
                     HEFYFGNAPEYLLYAYAQSSYNRGRRFGTRPLPEEDLAEYMRLYLELFNFATLPSFYW
                     ADYESTQGRLRLVDASKKIAEWLKENGVPVKGHTLVWGNPPSVGVPGWVERMGKLEQW
                     SEVRDLLFKRVAREVEEFKDLVQYWDVVNEPIVQNWFDSLGTDYIAQSYRIVKETDPD
                     AITVLNEYGVLVNTGTRRAFISRARQLINEGVQIDAIGAEAHIFTAQDLLGQLGSLES
                     IYLAIDELAQLGKPVHISEFQIPLPAVIDAFKVSISEAEEIQAEIAKIFYKVFFSHPA
                     VEVITYWNFTAPGSREVDSCAMICLSNRSSTN"
     gene            3829..3999
                     /locus_tag="PLD15_08375"
     CDS             3829..3999
                     /locus_tag="PLD15_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63963.1"
                     /translation="MKIDVGSSGEVAFKGFAGNYEITVATDGFSKTFSLDVGGKKENN
                     FVLVIGKEQEVR"
     gene            4000..>5560
                     /locus_tag="PLD15_08380"
     CDS             4000..>5560
                     /locus_tag="PLD15_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPB63964.1"
                     /translation="MRPSIAISIVLFMALLFVPASVLVGVDHVMAVNPINLGPVISPF
                     LYGVFFEDINHAVDGGLYAELVRNRSFEHTDRIEGWSVVLEKGCKASFSIKSERPVNG
                     NNTSYLRFDIESDDRRVILSNDGYDGIPLVGGESYIFSSFIRGDDYSGEIEIRLMDSQ
                     GNSIITASLGSKFADLWERYSVVLEVPEDCHDGRLALILKGSGLICLDMISLLPERNW
                     HGMRPDLLEMLKELKPGFLRFPGGCLVEGDSLENSYRWKDTIGPVEERKANYNLWGYN
                     QSYGIGFFEYLILAEYLGAEPVPIFNAGISCQVRGAEYCSMNTMDGWVQDVLDFIEFA
                     NGPTESSWGAKRAELGHPEPFNVTYIGVGNENWGNEYHERFALFQKAIKDKHPEIVIL
                     FSGPPSYEGASFNRAWRWARQNAVEIIDEHIYAVPEWMLRNTERYDKYDREGPKVMLG
                     EYAAHEAGRRNTLQAALAEAALMTGLERNSDVVIMAAYAPLFNRPGWSQWTPDLIWFD
                     STRVYGTPSYHV"
BASE COUNT         1297 a         1345 c         1510 g         1408 t
ORIGIN      
        1 atagcggcga tggaagctct gatcccgatt acccctgccg gattgactcc ctactacgag
       61 aatcccgcct ggacatggat atggttccat ctgctatggc agcacggcgg cgatcttctg
      121 accgatgact tcaaaaagcc ggccttcgct aaagcgggaa tcgaggcctg caaattgatg
      181 ctcacgatgc aggaaaaggg cattctcccg atgcaggtag ccgatcctgg cgtatcgttc
      241 cagtcgggcg atagcactgt tctgataacg ggtatctgga cgatccagcc ctggatggaa
      301 cagctcggcg aggccttcgg ctacgcggta tcacctcagc tgggtacgac caaggccgtc
      361 ttcggaggtt cgcacgttct ggcgatgccg aaggtaatgg ttgaagaccc gaaggttctg
      421 gacgccgcaa ctaccttcat gaaatacctc tgggacaacg cgatagactg gtatgcggcc
      481 ggtcagacac cagccagaat ctccatttcg cagagtcagg agctgaagga gagactgcca
      541 cacatttacg ttgtctctca ggaatcggaa tatgtaaagc agttccagat gttcccgttg
      601 atatcggaaa tcgaagccga aatcgctgta tatctcgacg aaatactggt gctgcactcc
      661 atctctcccg aagagggtat gaaagaagcc gaatttgcgg tgcaggagat tcttgacgat
      721 tactggtcaa gagcgaggta agctttcatt aattgggtga agggaggagg agcaattgag
      781 acaatcacaa tcgaagtcgc atagacgttt ccacagggag gtagggtcgt ggttgttcct
      841 cctccctcac ctgtttttct tcgtcttgtt cgtcgccatt cctctggtct ttgggatggt
      901 aatcagtttc ttcaactgga gtctgctcag cgacaatgtc tttgtaggag ccagcaacta
      961 cgtccgcaca tggaacgact cgagattctg gcctgttgtt cagaacactg tgatattcgc
     1021 gatagtaagc gttcccctta caataatagt ggccatactc tttgcccata tcctcaacaa
     1081 aaaaaggttc ggacagttat ggctgttaat cgcttttgtc tcaccggcct tcttcggatc
     1141 ggtgggcatt ctcagctcgt ggaaatggat attcgcttcc tttccatccg gtctggccaa
     1201 ttactacctg aacaaaaccg gtttcatcaa tggtgccgtt tcatggttcg gcagcactgg
     1261 agttgcctgg ggtgtgataa tactggtgac catttggtgg atagtggggt tcagcattct
     1321 gctctatatg ggcgcgttgc agaggatccc tcccgagcag tacgaatcgg cgaaactgga
     1381 tggagcggga ccatggaaga ggtttttgta cattaccttg ccctggataa ggagcgtact
     1441 cttcttcgat gtggtcagac aggttattct cgctttcggg cttttcgacc aggcttacat
     1501 actatcggtc ggcggtcctg ccggcaccac gagaacaatg gtctattacc tgtacctggt
     1561 ggggttcgag agacaggatt tcggaagggc cgcgtcgata tcctggttta ttttcgcgat
     1621 agtgcttatc tttgcggtaa tacagctcgt gctcgtgacg aagtcgatac gctcgacaga
     1681 ggggtgagag aatgacttct acagttgcca taaacaaaaa gaaactcagc aagcgcataa
     1741 ctgccgtcgt tgtaacgata cttctctggg catttgcggc tttttggttt gcgccgattt
     1801 tctggatgct gtcaacttcg ctgaagtcaa cggcagtggc tgtcattcag acgcctcccg
     1861 agtggatacc tgaaaacccc accctggaga attacaaaat cattttcgcg ccttcagggg
     1921 ggctttcgct catcagggca actctgaaca gcgtgatcgt cgccgtcggt tccacgctgg
     1981 caacgctggc gctttctatt ccggccgctt atgctctctc gcgtctgcga ttcaggggtc
     2041 gtatgcttgt tttctggatc tatgtggccg tgctcgcctt ccccggcgtg ctgtttttgg
     2101 tgcctcactt cttcataatt cagggaatgg gactgacaaa ctcctatcta gccctgattc
     2161 taccgggcct cgggggaact ttcggcgtct ttctctttag acagtacatg ctcgatattc
     2221 ccggcgagct ggaggatgcc gcctggatag acggctgttc gaagttcagg ttccttataa
     2281 gcatagtaat tccatatgtc aagccggcga tgcttgtgct gggcttgatg acctttctgg
     2341 gttcgtggaa cagttttctc tggccgcttt tgatactcag caagtccgac aaattcacgc
     2401 ttccgatagc gcttataaga ttcagtgccg gctggggcga cccctaccgc gggataggtc
     2461 cgatgatggc cggggccttc ttttcggttg caccgtcgct gataatcttc gttgtcttcc
     2521 acagatactt gatgaaaggg atatctctgg gatctcttgg aaaggagtag gtctcatgtc
     2581 gtttgagcag tcttcttcga agaagggatt tgtacttcct aactttatcg tgcggacctg
     2641 ttgtctgctt ttcatagcct tgttcgtaag cacttccgtc tttggaggta atgttttgcc
     2701 tgaagtcgac acaagtcttt tcgaggaaag gatagaaaac gatagaaaga taccgatcag
     2761 tataagcatc gtggatgagc aggggagacc ggtgagtaaa gcttttttga ctgcaaaaca
     2821 gctttcacac gagttttact ttgggaacgc tccggaatat ctcctgtacg cttatgccca
     2881 gtcaagttac aacaggggta ggagattcgg aaccagacct ctgcctgagg aagacctcgc
     2941 ggagtatatg cggctctatc tggagctctt caacttcgct acccttccgt ccttctactg
     3001 ggccgactac gaatccactc aagggcgtct acgcctggta gatgcttcaa aaaagatcgc
     3061 cgaatggctc aaggaaaacg gtgttcccgt gaaaggtcac acactcgtct ggggaaaccc
     3121 tccgagtgtc ggtgttcccg gctgggtgga aagaatggga aagctggaac agtggtcgga
     3181 ggttcgcgat cttcttttca aaagggtggc tagagaggtt gaagagttca aagatcttgt
     3241 ccaatactgg gatgtcgtta acgaaccgat cgttcagaac tggttcgact cactgggcac
     3301 ggactatatc gctcaatcgt acagaatagt taaggagacc gaccccgatg caatcacggt
     3361 tctcaacgaa tacggcgttc tggtcaacac aggtaccaga agggctttca tttctagagc
     3421 caggcagttg ataaacgaag gtgtgcagat agacgcgata ggtgccgagg cccatatctt
     3481 caccgcacag gacctccttg ggcagttggg atcgctcgag agcatttatc tggcgataga
     3541 cgaactggct caacttggaa aacccgtaca catttcggag tttcagattc ctcttcccgc
     3601 ggttatcgat gccttcaaag tgtctataag cgaggccgaa gagatccagg cggagatcgc
     3661 gaagatcttc tacaaagtct tcttcagcca tccggcagta gaagtcatta cttactggaa
     3721 ctttaccgcg cctggcagtc gggaagtgga ttcctgcgcg atgatctgtc tatcaaaccg
     3781 ctcttctacg aactgaagaa cttgatccat ggagaatgga agacggacat gaagatcgac
     3841 gtcggttcct ccggagaagt cgctttcaaa ggattcgccg gtaattatga aatcaccgtt
     3901 gccactgatg gtttttcaaa gactttctcc ctggatgtgg gcggcaaaaa ggaaaacaac
     3961 ttcgttctcg taatcggaaa ggaacaggag gtgcgatgaa tgagaccttc catagcgatt
     4021 tcgatcgtcc tgtttatggc tttgctgttc gttcccgcat ccgttctggt gggcgtcgat
     4081 catgtgatgg cagtgaaccc tatcaacctc ggccctgtaa taagtccctt tctctacggc
     4141 gttttcttcg aagatatcaa ccatgcggtt gacggtggtc tttacgccga gctggttaga
     4201 aaccgctcct ttgaacatac cgatcgcata gaaggctggt ctgtggtcct tgagaaaggc
     4261 tgcaaggcga gcttttcgat taaatccgaa agacctgtca acggtaacaa cactagttat
     4321 ctgaggttcg atatagaatc cgacgatagg cgcgtgattc tctccaacga tggttacgat
     4381 ggaattcctc tcgtcggggg cgagagttat atcttctcct cgttcatccg tggcgatgat
     4441 tacagcggag aaatcgaaat ccgcctcatg gattcacagg ggaactcaat aatcaccgcc
     4501 agcctgggat cgaaattcgc tgatctgtgg gagagatatt ccgtggtcct agaagttccg
     4561 gaggactgtc atgatggtcg gctggcgttg atcttgaagg gctccggtct gatatgtctg
     4621 gatatgatct cgctcctgcc agagaggaac tggcacggta tgaggccgga tcttctggaa
     4681 atgctcaagg aattgaagcc ggggttcttg aggtttcccg gtggttgtct ggtagagggt
     4741 gacagccttg aaaactccta ccgctggaag gacacgatcg gtccggtgga agagagaaaa
     4801 gctaactaca acctatgggg ctacaaccag tcctatggaa tagggttttt cgagtatctt
     4861 attctggctg aatatctggg cgccgagccc gtgccgatat tcaacgcggg aatctcctgc
     4921 caggttcgcg gggccgagta ctgttcgatg aatacaatgg acgggtgggt tcaggatgtt
     4981 cttgacttca ttgagttcgc caacgggccg acagaaagtt cctggggggc gaaacgtgcc
     5041 gaactcggtc atcccgaacc tttcaacgtt acatatatag gcgtaggaaa cgagaactgg
     5101 ggaaatgaat accacgagcg cttcgcactc tttcagaaag ccataaaaga caaacatccc
     5161 gagatcgtca tactcttcag cggaccgcct tcctacgagg gcgcatcgtt caacagggcc
     5221 tggagatggg cccggcagaa cgcggtggag attatcgacg agcatattta cgccgtgccc
     5281 gaatggatgc tgagaaacac cgaaagatac gacaaatacg atagagaggg tccaaaggtt
     5341 atgctcgggg aatatgcagc ccatgaggct ggaaggagaa acaccctgca ggcggcattg
     5401 gccgaagcgg cattaatgac cggactggaa cgcaatagtg acgttgtgat aatggcggcg
     5461 tacgctcccc ttttcaacag accgggctgg tcgcagtgga ccccagatct aatctggttc
     5521 gacagcacga gggtttatgg aacacccagc tatcacgtcc
//
LOCUS       DAOOLY010000162         5518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_17719_length_5518_cov_4.748124, whole genome shotgun sequence.
ACCESSION   DAOOLY010000162 DAOOLY010000000
VERSION     DAOOLY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17719_length_5518_cov_4.748124"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="PLD15_08385"
     CDS             <1..1547
                     /locus_tag="PLD15_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63965.1"
                     /translation="LITFIGALGGIGCSVTSSIAAQNAGADIRRNLFSKVQHLSFGSI
                     DRFGTGSLITRLTDDVVQFQHFIMLLLRMFVRAPLLFFGSVIMAFSISWRLSLIIVAI
                     IPLIVIIIYFSMKKVLPLFTRVQKEVDKVNSVVRDNVSGIRVVKSFASSNQESAKFAK
                     TNDEYMRLSMKAARILVVIMPLFSLILNFGIVAVIWIGGIQVEAGGLQTGQIMAFVNY
                     LTRMLMSIMMIGMMLIFVSRAQASARRISEVLETPEEEDIDTTEKFDLDGAIEFQNVN
                     FSYEGNERKVLHDINLTINPGETVAFLGDTGSGKSTLVNLIPRLYEVTSGRIMLNGID
                     ITKIPRRELRREIAFVFQETVLFSGKISDNIAYGNPDVSLEDIQQAADMARVGDFIST
                     LPDGYDTNLSQMATNLSGGQRQRVAIARALASGARIIVFDDSTSALDARTEAAVMKAI
                     NDRLKCTKIIIAQKISSVVNSDRIYLLEAGRVVERGTHGELMKTSDLYRDIYRSQFGE
                     VGEESA"
     gene            1531..3387
                     /locus_tag="PLD15_08390"
     CDS             1531..3387
                     /locus_tag="PLD15_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63966.1"
                     /translation="MRKVPEEKRERPTDIQINIGPRRGGPGAGRTAPVKKAEDPRKTL
                     FRLLAFLGHEKKGLLYVVIFTILSSIASIFGPFLLGKAIDDHILVGDLAGLAIIAILM
                     AAIYASSSLFQWLQGYVMIGVSQRTVRRLRQKVFDKYQTLPVKFFDTHSHGELMSRVT
                     NDINNISTTLSESVTQLLSSLLSLGGIGIMMFILSPILALVSLTSVPLVVLVTGLIAK
                     QSKKNFVSQQKALGELNGYIEERISGEKVVKAYGREEFTIAQFQEINESYRKSAILAQ
                     IFAGMFGPVMNMVNNFGYAIVAFAGGWMSIEGMISVGLVASFVVYVGHFNRPINQIAQ
                     LYNSIQAALAGAERVFEVLDEKGEKKSDEDFFPEKIEGKVELREVDFSYDGKTTILKK
                     VSLEARPGQIVALVGPTGAGKTTIVNLLTKFYDIESGTIYIDDHDISFFDREQLRRKL
                     GLVLQDTQLFMGTIRDNIRYGKPDAKDDEIRTVAKMANADHFIESLPEGYDTFISSNA
                     DSISKGQRQLLAIARIMLLDPEILILDEATSNVDTRTEMHIQEAMRNLMRGRTSFVVA
                     HRLSTIKNADKIYVINKGEIIESGTHSELITRGGFYNSLYKTQFSLPKEKTA"
     gene            3526..3987
                     /locus_tag="PLD15_08395"
     CDS             3526..3987
                     /locus_tag="PLD15_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB63967.1"
                     /translation="MEEKVRFVEVTEDNLIECLKLSKTLLENQDKAVASNAVSIAQAH
                     FAKNAWFRAIYAGDTMVGFIMLDYTPVPELGDIKHAFLWRFMIGGNYQKKGYGGDALR
                     LLFENLKEQGYEKLLTSCVVKEPSPLQFYLKLGFVDTGRWDDDEKILLRDL"
     gene            complement(4104..4179)
                     /locus_tag="PLD15_08400"
     tRNA            complement(4104..4179)
                     /locus_tag="PLD15_08400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4144..4146),aa:Pro,seq:cgg)
     gene            complement(4213..5250)
                     /gene="mnmA"
                     /locus_tag="PLD15_08405"
     CDS             complement(4213..5250)
                     /gene="mnmA"
                     /locus_tag="PLD15_08405"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB63968.1"
                     /translation="MKVGMALSGGVDSAVAAALMIESGYNVTGYHMIVLPSDGTTTQT
                     AEDAIRVAEHLGIELKIVDLSYQFRETVIEYFNGSYAAGLTPNPCVLCNDTIKFGILA
                     ERILADNNEAVATGHYARIISDEDGRFFLARASFDRKDQAYFLSRVKKEKLPFIRFPN
                     GELSKEEVRQKARSLAIPVHSKKDSQEICFIPDNDYRSFLHSQGIEGSTGDIVKGTGE
                     VIGRHNGLTDYTIGQRRGLGIHFREKLYVKDLDFNANRLVLGRREELVSFCLVAVDPN
                     WYIEPEREFDCLCKIRSSMNAVKAHVRLLEADRVAVSFPDGAWGVTPGQLAVLYSDEV
                     VLGSAFIERSC"
BASE COUNT         1458 a         1326 c         1394 g         1340 t
ORIGIN      
        1 tattgataac gttcataggt gcgctgggcg ggataggctg ttctgtgacc tcttcgatcg
       61 ccgcccagaa tgccggagcc gatatcagaa gaaatctctt cagtaaagtc cagcaccttt
      121 ctttcggttc aatagacagg ttcggtacag gcagcttgat aacaagactc accgacgacg
      181 tcgtccagtt ccagcatttc atcatgttgc tgctcaggat gttcgtcagg gcgcccctgc
      241 tttttttcgg aagcgtcatt atggcctttt ctataagctg gcggctttca ctgatcatcg
      301 ttgcgatcat tcccctgata gtgataataa tatacttttc gatgaaaaaa gtactgccgc
      361 tcttcacgag ggttcagaag gaagtcgata aggtcaactc agtggtgaga gacaacgttt
      421 cggggataag agtggtcaag tctttcgctt cttcgaatca ggaatcggcg aagttcgcca
      481 aaacgaacga cgaatatatg aggctatcga tgaaagcggc caggattctc gtggtcatta
      541 tgccgctctt ctcactgatt cttaacttcg gtatagtagc cgtgatatgg ataggtggta
      601 ttcaggtcga agcgggcggc ctccagaccg gtcagataat ggctttcgtc aattacctca
      661 ccagaatgct catgtccatc atgatgatcg gcatgatgct catcttcgtt tccagggctc
      721 aggcttccgc cagaaggatt tccgaggttc tagaaactcc cgaagaggaa gatatcgata
      781 ccacggagaa gttcgatctc gatggggcta tcgaattcca gaacgtgaat ttctcatatg
      841 aaggaaatga aagaaaggtc ctccacgaca tcaatttaac gataaaccct ggtgaaacgg
      901 tagccttcct cggagatacg ggctcgggta aatcgacact ggtcaatctg attccccggc
      961 tctacgaagt gacttccggt agaattatgc tgaacggtat cgatataaca aagattccca
     1021 gaagggaact caggagagag atagccttcg tctttcagga gacggttctc ttctccggaa
     1081 agataagcga caacatcgcc tacggtaatc ccgatgtttc actggaagat atacagcagg
     1141 cggccgatat ggccagggtc ggagatttta tctcaacgct tcccgacggt tacgacacaa
     1201 acctgagcca gatggcaaca aatctctcgg gcggtcagag acagagagtc gccattgcca
     1261 gagctctcgc ctccggagcg cgaatcatag tctttgacga cagtaccagc gctctcgatg
     1321 cccggacgga agctgccgta atgaaggcga taaacgacag actcaagtgc accaagatca
     1381 taatcgccca gaagataagc tcggtagtca attcagacag gatatacctc cttgaagcag
     1441 gccgcgtagt ggaaagagga actcacggcg agcttatgaa aacgagtgat ctttacagag
     1501 acatctaccg atcgcagttc ggagaagttg gtgaggaaag tgcctgaaga gaagagagaa
     1561 agacctaccg acatccagat caacatagga ccccgcaggg gcggacccgg cgccggaaga
     1621 acagcgccgg tgaagaaggc cgaagaccca cgcaagacgc ttttcagact gctggcattt
     1681 ctcggtcacg aaaaaaaggg tcttctttat gtagtgattt tcactattct ctcctctatc
     1741 gccagtatct tcggcccctt tctcctcgga aaggccattg acgatcacat actcgtggga
     1801 gacctcgcgg ggctggccat tattgcgatc ttgatggccg ccatttacgc ctcgtcttcc
     1861 cttttccagt ggcttcaggg atacgtcatg atcggtgttt cacagagaac ggtacggcga
     1921 ctcaggcaga aagtcttcga caaataccag acgcttcctg tgaaattctt cgatacgcac
     1981 tcgcacggtg aattgatgag cagggtgacg aacgatatta acaacatctc taccacgcta
     2041 agcgaaagcg tgactcagct cctatcaagt ctcctttctc tgggcggtat aggaataatg
     2101 atgttcatcc tcagccccat actggctctt gtgtcgttga cctcagtccc tctggtagtt
     2161 ttggttacgg gcttgatcgc aaaacagagt aaaaagaact tcgtttcgca gcagaaagcc
     2221 ctgggagagt tgaacggcta tatagaggag aggatctccg gagaaaaggt tgtcaaagcg
     2281 tatggaagag aagagttcac gatcgcgcag ttccaggaga taaatgaatc ctaccgaaag
     2341 tcggctatcc tcgcccagat attcgcaggt atgttcggtc cggtgatgaa catggttaac
     2401 aacttcggat acgccatcgt tgccttcgcc ggagggtgga tgtcgataga gggaatgata
     2461 agcgtcggcc ttgtggccag ctttgtggtt tacgtcggcc atttcaacag gcccatcaac
     2521 cagatcgcac agctttataa ctccattcag gccgctcttg caggagccga aagggtcttt
     2581 gaggttctgg acgaaaaggg agagaaaaag tcagacgagg acttcttccc cgagaagata
     2641 gaaggaaagg tggaactgag ggaggtagat ttcagctacg acggaaagac gaccattctc
     2701 aaaaaggtaa gcctggaggc tcgaccgggc cagatcgttg cgctggtggg tccgaccggt
     2761 gccggtaaaa ccacgatcgt gaatctcctg accaagtttt acgacatcga gagcgggacc
     2821 atttacatag acgatcacga tatatccttc ttcgacagag agcagttgag aagaaagctg
     2881 ggtctagtgc ttcaagacac acagcttttc atgggaacga taagggacaa tatacgctac
     2941 ggcaagccgg atgccaaaga tgatgagatc cgcacagtcg caaaaatggc caacgccgat
     3001 cacttcatcg agagccttcc ggagggctac gatactttta tatcttcgaa tgccgacagt
     3061 ataagcaaag gtcagaggca gttgttggca atcgcgagga taatgcttct cgatccagaa
     3121 atactgattc tggatgaagc caccagcaac gtggatacca gaacggagat gcacatacag
     3181 gaagcgatga gaaatctgat gcggggcagg accagctttg tcgtcgccca tcggctgagc
     3241 accataaaaa acgccgacaa aatatacgtc ataaacaaag gcgagataat tgaaagcggt
     3301 acacacagtg aactaatcac ccggggcggt ttctataaca gtctctataa gactcagttc
     3361 tcgctgccga aggaaaagac cgcttgagga ggcgaagatg attttcaaaa acttattttc
     3421 gaagcgaggt ttcaagtgtc cattcataag gttgatagaa aagatgaaaa gtaagaagcc
     3481 caaattctac gttgaatgaa gcatggcgct caccggaggt atattatgga agaaaaagtg
     3541 agatttgtag aggtaacaga ggataatctg attgaatgtc tgaaattgag taagactctt
     3601 ctggaaaacc aggacaaagc cgtcgcatcc aacgcagtgt cgatagcgca ggcgcatttt
     3661 gcgaagaatg cctggtttag agcgatatac gcaggggata cgatggtggg tttcataatg
     3721 ctagattata cgcccgttcc cgagctgggc gatatcaagc acgcctttct ctggcgtttc
     3781 atgatcggcg gcaactacca gaagaagggt tacggcggcg atgcgctccg tcttctcttt
     3841 gaaaacctca aagagcaggg atacgagaag ctcctgacct cgtgcgtcgt taaggagccc
     3901 agcccgcttc aattctacct gaagcttggc tttgttgaca ccggcagatg ggacgacgac
     3961 gaaaaaatcc tgctcagaga tctctaagca tccatcaaga acagatatcg attcgcactg
     4021 tggagttttc cgacagtggt agttagtcga tccgacttaa atatattgca aacggcagac
     4081 cattaatggt ctgccgtttt atctggtcag gaagcgggga tttgaacccc ggacctctgg
     4141 ttcccgaaac cagcgttctt ccagctgaac tacttcctgt ctgaaaaatt ctatcacgtg
     4201 gagcacgcag tttcaacagc ttctttctat aaaggcactt ccaagcacga cttcgtcgct
     4261 gtagagtaca gccagctgtc cgggggtcac cccccatgcg ccatctggaa agcttacggc
     4321 gactctatcc gcttccagaa gcctgacatg agccttaaca gcgttcatag aactacgaat
     4381 cttgcacaga caatcgaact ctctttccgg ttctatgtac cagttcggat ctacagcgac
     4441 cagacagaag gaaaccagtt cttctctgcg gcccagaacg agtctgttag cgttgaaatc
     4501 gagatccttc acatataatt tttctctgaa gtgtatcccc agcccccttc gctggcctat
     4561 cgtgtaatcg gtgagaccgt tatgacggcc aattacttca ccggttcctt tgactatatc
     4621 accggtggag ccttctattc cctgactgtg aagaaagctc ctgtaatcgt tgtcgggtat
     4681 gaagcagatc tcctgggaat ccttcttcga gtgtaccgga attgccagcg aacgcgcctt
     4741 ctgtcgtact tcttctttcg agagttcacc gttcggaaag cggatgaagg gaagtttctc
     4801 cttcttcact ctcgagagga agtaggcctg atctttcctg tcaaagctcg cccgggcaag
     4861 aaagaaccgc ccatcttcat cactgattat cctggcgtaa tggcctgtgg ccaccgcttc
     4921 gttgttatct gccagaattc gttcggcgag gattccgaac tttatcgtat cgttgcacag
     4981 aacgcacggg ttgggggtga gcccggcggc gtaactgccg ttgaaatact ctatcaccgt
     5041 ctctctgaat tgatagctga gatcgactat cttcagttcg atacccaggt gttccgcaac
     5101 cctaatcgca tcttcggcag tctgggttgt cgttccgtct gacggaagta ctatcatgtg
     5161 gtatcccgta acgttgtatc cggattctat catcagtgcc gcggcaacgg cgctgtccac
     5221 accgccgctc agtgccatac cgaccttcat ctttcatcac ccagacaatg ttagcagcct
     5281 tttttggtga gagtgttgcg aaagggtata ataaaagtgt gaagaatggc cgaaaggata
     5341 gttcgttgtt tgagacaatc gttgcactat tgaacttgtt tagttccatt acggaaaaac
     5401 gcgggtatca caaattcatt tttttgagtt cataatgact tcatcttcaa tcaaaaggag
     5461 gggtactatg aaaaagatct tgcttgtatc actcgtgatt cctgtttcgg caatttcg
//
LOCUS       DAOOLY010000163         5493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_17811_length_5493_cov_4.935822, whole genome shotgun sequence.
ACCESSION   DAOOLY010000163 DAOOLY010000000
VERSION     DAOOLY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17811_length_5493_cov_4.935822"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLD15_08410"
     CDS             <1..1638
                     /locus_tag="PLD15_08410"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB63969.1"
                     /translation="VVAAIVSIGWACTTIIVTKGASVDGSVMTSHSCDCGACDFRYVY
                     VPAADYEPGAQRAVYPFLEGYPRYVGKDMGPTYDDPNYPPTEPLGYIDQVEHTYAYYD
                     GAYGIINEHQLAIGECTCSAKVYAQAKAGECIFDVSALSKVALERCTTAREAIELMGA
                     LAVEYGYYGWGETLTVADTEEAWVFEICATPDQKSALWAAKKVPDGEVFVEANIFRIR
                     ELDPNSEDNMFSPNLIEVATAAGWYDPSSGPIDWMATVSTGEYSYPYYSLRRIWRVLD
                     RVAPSLELSPWVEDGFTKDYPFSVVPDKKLSVADVIDLFRDFYEGTEFDLTQGLAAGP
                     FGNPNRYSGSSRLIKGSWERAISIFRCDYVFVSQSRGWLPDPIGGVIWWGPSAPHESI
                     IIPMYCGITDIPDAYDTGSLEKFDYNAANWALNFMGNWAELKFSYMYPEIKALQQKIE
                     GKLFAVQPAIEAAAAQLYAIDPELCKEFLTDYVAGVSNRVMAEVWEFNEYLITKYRDG
                     YINIPNVGASAGYPDAWLKEVGYADGHIFGPDGYKAK"
     gene            complement(1851..2204)
                     /locus_tag="PLD15_08415"
     CDS             complement(1851..2204)
                     /locus_tag="PLD15_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63970.1"
                     /translation="MRRFKAISGELIVVAGTLLVAMAFFLVFTWGDSAVTMTYITVTD
                     KIDNSREGKFDFTVVAEGGEVFALRNSESHGIYSASKVFKTLEIGKRYQVLVTGRRLP
                     LLGLKRNIIQASLVQ"
     gene            2303..3340
                     /locus_tag="PLD15_08420"
     CDS             2303..3340
                     /locus_tag="PLD15_08420"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB63971.1"
                     /translation="MQTEMIRLDPENPGVDGIEKAAGLIKAGELVVFPTETVYGLGAS
                     ALDPGAVEKIYRAKGRPQDNPLIVHVSSLEMAGECTVQDLGRYSSILDVLWPGPVTLI
                     FKKSEAIPACVTAGLDSVGLRFPSHPVALRLIEAAGVPIAAPSANISGRPSPTSEEHV
                     IEDMNGRVGGIILAGDTLFGLESTIVDLSREKPTLLRPGPVDAERLGELLPGLEIPGF
                     VRARDEYRGEALSPGLKYRHYSPEIPLILIEGSPGNVVSRAGILARRDKSVVLCSQEL
                     ADYYPVHVKKVVLGSRNDLYAVASGLFKALRDRDNLNYTQIISEPFPETGIGLAIMNR
                     LRKAAWRIYRV"
     gene            3375..4097
                     /locus_tag="PLD15_08425"
     CDS             3375..4097
                     /locus_tag="PLD15_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB63972.1"
                     /translation="MLVLAHRGMGKGRYENTLKSFKEGLGHGAHGIETDVRLTGDGVV
                     ILSHDATLKRTMGLETVVAEKTLGELDSMGLIGEDGLTTLEELYRTLPGDKYFDVEIK
                     APEAVPGVIDIVRKFDALDRTMFSSFHHECLDAFRKAFGDKTMIAPIIDREVLKEGAE
                     EFLDETVKKYGPFALNLNKELFNYMGIERGREMLEKFRAEGVKISLWTLNDPELFAQV
                     RDVCDYLITDRSDIMMQFCRNH"
     gene            4114..4617
                     /locus_tag="PLD15_08430"
     CDS             4114..4617
                     /locus_tag="PLD15_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63973.1"
                     /translation="MKRIPLFFTVLLMILAITSCDKKAPENTESVSLSKGEILSLLGI
                     EEKEIVFSKVELDLSGKPLLTIAAYDKESRELFSRDFSQREIKGGYITVAVLKSPVIN
                     ALRFLYAITDRNGVQEAYVQDLPYYQYQQVEIEKAQFSKITSKGEFKILTASFGQNQQ
                     NIYLKVK"
     gene            4644..5333
                     /locus_tag="PLD15_08435"
     CDS             4644..5333
                     /locus_tag="PLD15_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HPB63974.1"
                     /translation="MRKILLVVIIVASFTAFGASIPRDRIQKGLEVIVELSGTEDSGS
                     FIELLRKSNGIVIYPDMVKAALGFGGQYGEGFLLRRDEEGRWYGPLFVKLYGISYGFQ
                     AGVQKSGLVLVIMNKQGIEGFLGNNITLGGSASVSAGPTGKTLAADTDYKLEAAIYSY
                     SVSNGLFAGISLGGSIIRQDSDADREYFGQSLTPREIIDTPASGKEIEELIAYLNGLT
                     NPAGGENPTIE"
BASE COUNT         1384 a         1420 c         1564 g         1125 t
ORIGIN      
        1 gttgttgccg cgattgtaag catcggttgg gcatgcacga ctatcatagt tacgaagggt
       61 gcttccgttg acggatcggt tatgaccagt cattcgtgcg actgtggagc ctgtgatttc
      121 agatatgtct atgttcctgc cgcagactat gaaccgggtg cacagagagc cgtttatccg
      181 ttcctggaag gatatccccg ttatgtgggc aaagatatgg gaccgaccta cgacgatcca
      241 aactatccgc cgacagaacc tctcggttat atcgatcagg tcgagcacac atacgcttac
      301 tacgatggcg cttacgggat catcaacgag caccagctcg cgataggcga gtgtacctgt
      361 tcggcaaagg tttacgcgca ggccaaagct ggcgaatgta tcttcgacgt ttccgcgctc
      421 tcgaaagtgg ctctcgagag atgcacgacg gccagagagg ctatcgagct catgggcgcg
      481 ctggctgtag agtacggcta ctacggctgg ggcgaaactc tcactgtcgc agataccgaa
      541 gaggcgtggg tcttcgagat ctgtgcgaca cccgaccaga agagcgctct ctgggcggcc
      601 aagaaagtcc ccgacggaga agtcttcgtt gaggccaaca tcttcaggat cagggaactc
      661 gatcctaaca gcgaagacaa catgttctcc ccgaacctga tcgaagtcgc gacggcagcc
      721 ggctggtacg atcccagctc cggtcctatc gactggatgg caaccgtcag caccggcgaa
      781 tactcctacc cctactacag tctcagaaga atctggagag ttctcgacag agtggctccg
      841 tccctggaac tttctccgtg ggtagaagac ggtttcacaa aggattaccc gttctctgtg
      901 gttcccgaca agaagctctc cgtggccgat gtaatcgacc tcttcagaga tttctacgaa
      961 ggcacggaat ttgatctgac acagggtctc gccgcaggac cgttcggcaa ccccaaccgc
     1021 tattcaggct cgagcagact gatcaagggc agctgggaaa gggctatctc catattcaga
     1081 tgcgactatg tgttcgtatc tcagtcgaga ggctggctcc ccgatcctat cggtggagtg
     1141 atatggtggg ggccgtcggc accgcacgaa tcgataatta tccccatgta ctgcggtatt
     1201 accgatattc cggatgcgta cgatacggga agtcttgaaa agttcgacta taacgctgcc
     1261 aactgggcgt tgaacttcat gggtaactgg gccgagttga agttcagcta tatgtacccg
     1321 gaaatcaagg cgctccagca gaagatcgaa ggcaagctct tcgcggtaca gcctgcgata
     1381 gaggcggctg cggcacagct ttacgcgata gatcctgaac tctgtaagga attcctgacg
     1441 gactacgtcg ccggtgtatc gaacagagta atggcggaag tctgggaatt taacgaatac
     1501 cttataacca agtaccgcga cggctacatc aacatcccca acgttggcgc gtcggcaggc
     1561 tatccagatg catggctcaa ggaagtcgga tacgccgacg gtcatatctt cggaccggac
     1621 ggttacaaag ccaagtaaat cttctcgcct gccccgtcaa aagggcaggc gattcttctt
     1681 aaatataaac gactctaccg caactgcgac aatcaaaaga gatattgccg ggcttttcta
     1741 ccgaagaagg caacggcaga tggatcaatt tggaggcggt tccacggttc tctaagtgga
     1801 ttttcgcgag agttccgtaa aaaccggcag cggatggttt cgatatagat ctattgaacg
     1861 agtgaagcct ggatgatgtt tctcttcagg ccgagcaagg gaagccttct gcccgtgacc
     1921 agcacctggt atctcttgcc gatctctaga gtcttgaaga cctttgaagc cgagtaaatc
     1981 ccatggctct ccgagtttct caacgcgaaa acctcgccgc cctcggccac aacggtgaaa
     2041 tcgaacttcc cttcgcgcga attgtctatc ttgtctgtaa cggtgatgta ggtcatggtc
     2101 accgcgctgt ctccccacgt gaagaccagg aaaaaggcca tagccaccag gagcgtcccg
     2161 gcgacgacga tcagctcgcc ggatatagcc ttgaatcttc tcattattcc aacctcgctt
     2221 tcccatcgag aaatataaca cacacaaccg gaaaagacat gaatgagata acgttgtatt
     2281 atttatcgtg aggaggctcc atatgcagac ggagatgata aggctcgacc cggagaatcc
     2341 gggggtcgat ggcatagaaa aagcggccgg tctcataaag gccggtgaac tcgtcgtctt
     2401 tccgaccgag accgtgtatg gcctgggagc cagcgcgctc gatcccggag cggtcgagaa
     2461 gatataccgg gccaagggaa ggccccagga caacccgctc atagtccatg tttcttcgct
     2521 ggagatggca ggagaatgca ccgtgcagga cctggggcgg tactcttcca tactggatgt
     2581 gctctggccc ggtccggtga ccctcatatt caaaaagagc gaagcgatcc cggcatgtgt
     2641 gacggcggga ctggacagcg tcgggctgag gtttccttcc catcccgtag cgttgaggct
     2701 catcgaggcg gccggggtac cgatcgccgc gcccagcgcc aatatatccg gcaggcccag
     2761 tccgacctcc gaggaacacg tcatagagga catgaacggc cgcgtgggcg ggataatact
     2821 cgccggagac acgctcttcg gcctggaatc tacgatagtg gatctctcca gagagaagcc
     2881 gactctgctc cggcccggcc cggtggatgc cgaaagactt ggcgaattac tgcccggact
     2941 cgagatcccc ggtttcgtcc gggcccgcga cgagtacagg ggcgaggcgc tctcgcccgg
     3001 tttgaaatac cgccactact cgccggagat tccactgatt ctgatcgaag ggagcccggg
     3061 caacgtggtc tctagggccg gaatccttgc cagaagggat aaatcggtcg ttctctgctc
     3121 gcaggaactg gccgattact acccggtaca cgttaaaaag gtcgttctcg gctcgagaaa
     3181 cgacctctac gcagtcgcgt ctggcctctt caaagccctg cgcgaccggg acaaccttaa
     3241 ctacacacag atcatctcgg agcccttccc ggaaacgggg atcggcctcg ccataatgaa
     3301 cagactgagg aaagcggcct ggagaatata cagagtataa atgagagtat tcgactgatc
     3361 ggacggaggt aaatatgctg gttctggcgc acaggggaat gggaaaggga agatacgaaa
     3421 acactttgaa atccttcaag gaggggctgg gtcacggcgc acacggaata gagaccgacg
     3481 tgaggctcac cggggacgga gtggtgatcc tcagtcacga cgcgaccctg aagcgtacca
     3541 tgggactgga aacagtggtg gccgaaaaga ctctcgggga gctcgactct atggggctga
     3601 tcggcgaaga tgggcttaca acgctggaag agctttacag gaccctgccg ggcgataaat
     3661 acttcgacgt cgaaataaag gccccagaag ccgttcccgg cgtgatagac atcgtgagaa
     3721 agttcgacgc actcgataga accatgttct catcctttca ccacgaatgt ctggacgctt
     3781 tcagaaaggc cttcggcgac aaaaccatga tcgcacccat aatagacaga gaggtgctga
     3841 aggagggggc cgaagagttc ctggacgaaa cggtgaaaaa atacggaccc ttcgcgctca
     3901 acctgaacaa agagcttttc aactacatgg ggatcgaaag gggaagggaa atgctcgaaa
     3961 agttccgcgc ggagggcgtc aaaatatcgc tctggactct caacgatccg gagctgttcg
     4021 cgcaggtgag ggacgtctgc gattacctga tcacagaccg ttcagatata atgatgcagt
     4081 tctgccgaaa ccattgattg gaggattggc tgcgtgaaga gaatacccct gtttttcacc
     4141 gtattgctta tgattttagc tataacttcc tgtgacaaaa aagctccgga aaacaccgag
     4201 agcgtctcgc tatcgaaagg agagatactc tcgctattgg gtatcgaaga gaaagagatc
     4261 gtcttctcga aggtagagct cgacctgtcg ggcaagccgc ttctgacgat agcggcttac
     4321 gacaaagagt cgcgggaact tttctccaga gacttctcgc aacgcgagat caaggggggt
     4381 tacatcacgg tcgcggttct gaaatcgcct gttataaacg ccctcaggtt tctctacgcg
     4441 atcaccgaca ggaacggggt acaggaagcg tacgtacagg acctccctta ctaccagtac
     4501 cagcaggtcg aaatagagaa ggcacagttt tcgaaaataa cttccaaagg cgagttcaaa
     4561 atactgacgg ccagtttcgg acagaatcaa cagaatatat acctcaaagt caagtgagaa
     4621 cctgctccta aaggaggttt cagatgagaa aaatcttact ggtggtgatc attgtggctt
     4681 cattcacggc cttcggggca agcatcccga gagacaggat acaaaaggga cttgaagtga
     4741 tcgtagaatt atccggcacg gaggacagcg gctcctttat cgaactgctg cgcaaatcca
     4801 acgggatagt catatacccg gatatggtga aggcggcgct cggttttggg ggccagtacg
     4861 gcgagggctt cctactgcgc agggatgagg agggcagatg gtacggtccg ctcttcgtca
     4921 aactctacgg cataagctac ggctttcagg ccggagtcca gaaaagcggc ctggtcctgg
     4981 tgattatgaa caaacaggga atcgaaggct tcctgggaaa caacataaca ctgggaggca
     5041 gcgcctccgt atcggccggc ccgacaggaa agacgcttgc ggccgacacc gactacaagc
     5101 tcgaagcggc catctattcc tattcggtca gcaacggcct cttcgccggc atttctctcg
     5161 gcggctcgat cataaggcag gactcggacg cggacaggga gtacttcggc cagagcctga
     5221 caccgcggga aataatagac acgcccgcat cgggaaagga gatcgaagag ctgatcgcct
     5281 acctcaacgg actcacaaac cccgccgggg gcgaaaaccc gaccatagag tgataaagag
     5341 gggttgtccg taatgccccc attttgtgct cttctcggag gacggtggac ggcgtgtgga
     5401 acagaggtaa gaggtatgag gtaagaggta tgaggtaaga ggtatgaggt atgaggttag
     5461 aggtatgagg ttagaggtgt gaagatcaag atc
//
LOCUS       DAOOLY010000164         5422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18132_length_5422_cov_3.832681, whole genome shotgun sequence.
ACCESSION   DAOOLY010000164 DAOOLY010000000
VERSION     DAOOLY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18132_length_5422_cov_3.832681"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..423)
                     /locus_tag="PLD15_08440"
     CDS             complement(85..423)
                     /locus_tag="PLD15_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPB63975.1"
                     /translation="MECVFCKIAGGHIKSALVSVNEDFVAFDDINPVASCHVLVVPKR
                     HIKSIKELSGFSGQALKNLLDIIDIVADEKGVKETGYRVIVNQGSDAGQEVEHLHFHV
                     IGGRKLGRLG"
     gene            complement(436..1098)
                     /locus_tag="PLD15_08445"
     CDS             complement(436..1098)
                     /locus_tag="PLD15_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HPB63976.1"
                     /translation="MILALGIFAPEVKPIIDQMLIIESGEILGRSYSRGVIGNNEVVA
                     CSGFVGKVETAFMTQKLIDHFRPRLVMLLSGAAALDQSLKPGDLVIGTEFEEYDTVFP
                     LSHGKMVIKAPDNLVMRFLKIYFKDWRFGKIVSGDGVVYSVEERDKIASSHRALALDM
                     DSAAFAKMANENNQSYVVIKTILDMADENTQSDFDRNFPLFCGKPARLMVEMMKTHYI
                     DR"
     gene            complement(1153..1536)
                     /locus_tag="PLD15_08450"
     CDS             complement(1153..1536)
                     /locus_tag="PLD15_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPB63977.1"
                     /translation="MEERDELELHNCEDCCDGDCDCNHEIEPERFSVTDEDGIEHHFE
                     IIYELENDGKFYWITQEFFEGEDGEPIEDENESEYVVFRAEYSEDDDPVLYSVEDEEF
                     EKVSVAWNKIVEEMLAQADDTEEEE"
     gene            1651..2226
                     /locus_tag="PLD15_08455"
     CDS             1651..2226
                     /locus_tag="PLD15_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63978.1"
                     /translation="MLEIERKFLYSGSMNDSTFEKAEVCLLIIQWYLSPPDTRRLRLA
                     VSPESSRMIETVKTGFGMVREESEREISVEILHEMAYRLKCEKAVVKARYVYPRKSFE
                     GVIDRYFVPDIGFVVEIETSQIQNVIPDPWVFWDLPAGYFMEVTENPIYTARSLAVKI
                     SDTTGLLPSSIPLVEPFQRQEFERSLSIVYG"
     gene            complement(2249..3583)
                     /locus_tag="PLD15_08460"
     CDS             complement(2249..3583)
                     /locus_tag="PLD15_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPB63979.1"
                     /translation="MHKQYFMVPEGTSIEMLSPKFWTRRITGQGKRWLKESELSAFNE
                     ELLTNAERTGLERFYRNLEEFPTRVELDSIRAMIGETIPEGFFTRTLVSPEGEEVPAE
                     IRDDILENATNLMNQDMVLQMGLTTRRTHLRAMPTDLILVEGFLDNDDLQLTSVSLGS
                     PFVALARSRDKSWLFVQTRTYRGWIKGDHVAVAKNRSDVIYYCSGEEFLLACGSRVEI
                     EPDPFLPDTWDLFLQMGDRLPLEKPKDVENPHSQGPYGCYAVKIPFRNRKGTLEFRTG
                     LIRASEDVSRGFMKLTTKNVLKQAFKMLGERYGWGGSYNRRDCSRFIQDIYKCFGLEL
                     PRDSGIQEKLIPSPRIEFTGSAEDREMQLASLRPGNPLYMPGHTMIYLGRCDGRFYVI
                     HDGSGYTDRHDRQVSVHGVFVMELSLRTIKSQKSYIELLTSAPEIDKGLLKR"
     gene            complement(3606..4436)
                     /locus_tag="PLD15_08465"
     CDS             complement(3606..4436)
                     /locus_tag="PLD15_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB63980.1"
                     /translation="MKAKTKTNIKKAISLLILAILASLAFIPLYWMVATAFTRPSLTM
                     KFPPDVIPKNPTLQNFKDLFERSIIFRWTLNSFIVAGTVTIAQIFVCAMAGYSLAKKR
                     FPGSKALFSIYIASMMIPTQVTLVPLYILISKMGMVDTYQALILPAIAAPFGVFLMRQ
                     FMVSVPTEIIEAARIDGAGEFRTFIEIIVPISKPAMAVLGIFTFMGQWNSFLWPLIVT
                     NTTQMRTLQAGLSLLQEQVPMQYSYLMASATYSAIPMIIVFLAFQKYFLKGITVGAIK
                     "
     gene            complement(4448..5083)
                     /locus_tag="PLD15_08470"
     CDS             complement(4448..5083)
                     /locus_tag="PLD15_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB63981.1"
                     /translation="MWLGKALLVTVLIYPFRKSIRTFFKAAFYLPHVTSSVIISMIWL
                     WVFNPNFGLLNYLMTLLGLDPVIWLGRSLTAMPSLIIMQVIMGGGSTIVLLSAAMASI
                     PEYYFEAATLEGASSWAIFRKITVPLLKPTILYALVMGTIANFQTFSNIYIMTSGGPE
                     FSTTTIAYLIYETAFENYNLGLASAMSMVMFVILVVLGVIQFKWLGSNVEY"
BASE COUNT         1373 a         1397 c         1245 g         1407 t
ORIGIN      
        1 gaccgttttt gagatcgaac ttcactgtct tgatcgttct cggcggttgt ttttgatcag
       61 tgtaatagtg ctcaaagcct tctttcatcc cagtcttccg agtttccttc cgccgataac
      121 gtgaaagtga agatgctcca cttcctgacc ggcatcgctt ccttgattga cgatcaccct
      181 gtaacccgtc tccttgacac ctttttcgtc ggcgacgata tctataatat cgagcagatt
      241 cttgagcgct tgaccggaaa agccagacag ctctttgata ctttttatgt gcctcttcgg
      301 aaccactaga acatggcagg aagctacggg atttatatcg tcgaaggcta cgaaatcttc
      361 gtttacggaa acgagggcgc tctttatatg accgccagcg atcttacaga acacacattc
      421 cataatccac actccctatc tatctatgta atgggttttc atcatctcta ccatcagccg
      481 ggcaggtttt ccgcagaaaa gcggaaagtt cctgtcgaaa tcactctgtg tgttttcgtc
      541 ggccatatcg agaatggtct ttatcacaac gtaactctgg ttgttctcgt tagccatctt
      601 ggcgaaggct gccgaatcca tatccagagc cagtgctctg tgggagctcg ctatcttatc
      661 tctctcttcg acgctgtaaa ctacgccgtc gcccgagacg atcttgccga acctccagtc
      721 cttgaaataa atcttcaaaa acctcataac cagattatcc ggtgccttta tgaccatttt
      781 tccgtggcta agagggaaga cggtgtcgta ctcttcaaac tccgtgccta tcaccaggtc
      841 gcccggcttt agagactgat caagagcggc cgctccggag agaagcatga ccagcctggg
      901 tcgaaaatgg tctatcagct tttgggtcat gaaggcggtt tccacctttc ccacgaagcc
      961 gctgcaggca acgacttcgt tgttacctat gacacccctg ctataactcc ttccaagaat
     1021 ctctcctgat tcgataataa gcatctggtc tataataggt ttcacttctg gtgcaaagat
     1081 acccagagcg agaatcatcg gatcaccccc acaagagatt ataacaaaaa aggggacccg
     1141 gggtcccctc tcttattctt cctcttctgt gtcgtccgcc tgggcgagca tctcttcgac
     1201 tattttgttc caggcgacac tcaccttctc gaattcttcg tcttctaccg aataaagcac
     1261 cggatcgtca tcctcactgt actccgctct gaaaacaacg tattccgatt cgttctcatc
     1321 ctctatgggt tccccatctt cgccttcgaa gaactcctgg gttatccagt agaacttgcc
     1381 atcgttttcc aattcgtaaa ttatctcgaa atggtgttca ataccatctt catcagttac
     1441 ggagaatctt tcgggctcaa tttcgtggtt gcagtcgcag tctccgtcgc agcagtcttc
     1501 acagttgtgc agttccagtt catctctttc ttccatcgta aacctccatt atttgcgatt
     1561 cggttgatac taacacagta agtggaaagt cgcaacaagc aaagcgtagt agaattgctt
     1621 aagaaaactt cgtcgaagag aggtcgcaaa atgctagaga ttgaacgaaa gttcctttac
     1681 agtggaagta tgaacgattc aactttcgag aaggcggaag tctgtctcct cattatacag
     1741 tggtatctga gtccgcccga caccaggaga ctccggcttg ccgtttctcc cgagagctcc
     1801 agaatgatcg aaaccgtgaa gaccgggttc ggaatggtca gagaggagag cgaacgggaa
     1861 atctccgtag agatccttca tgaaatggcc tatcggttga agtgcgagaa ggcagtggtg
     1921 aaagcgaggt acgtttaccc gcgaaagagt ttcgaaggtg tgatagacag atacttcgtt
     1981 cccgatatag gctttgtcgt cgagatagag acatctcaga ttcaaaacgt catacccgac
     2041 ccctgggtgt tctgggacct accggccgga tatttcatgg aagtaaccga aaatccgatt
     2101 tacacggctc gctcactggc tgtcaaaata agcgacacaa ccggacttct tccatccagc
     2161 atcccactcg ttgaaccctt ccagagacag gaatttgaac gatctctctc tatcgtttac
     2221 ggctgaactt ccgaagttct cgcctccgct accttttcaa aagtccctta tctatctctg
     2281 gagcgcttgt gagtagctca atatagcttt tttggctctt aatggttctg agggagagct
     2341 ccataacaaa aaccccgtgt accgagacct gcctgtcatg cctgtcggtg taaccacttc
     2401 cgtcgtgtat cacgtagaag cggccgtcgc atcgccccaa atagatcata gtgtgtccgg
     2461 gcatataaag gggatttccc ggtctcaaag aggctagctg catttctctg tcttccgcac
     2521 tcccagtaaa ctcgattcgg ggagagggta tcaacttttc ctgaattccg gaatctctgg
     2581 gcagttccag gccgaaacat ttatatatat cctggatgaa ccgcgagcag tcgcgcctgt
     2641 tataggagcc gccccatcca tatctttcgc cgagcatctt gaaggcctgt ttgagtacgt
     2701 tcttggtagt caatttcatg aaacccctgc taacgtcctc actcgcccgg atgagcccgg
     2761 ttctaaattc cagagttccc ttgcgattac gaaatgggat cttcaccgcg taacagccgt
     2821 aagggccctg ggaatggggg ttttcgacat ctttaggctt ctccaggggt aatctgtcac
     2881 ccatttgaag gaagaggtcc catgtatcgg gaagaaacgg gtcgggttct atctctaccc
     2941 tgctcccgca ggcaagcaaa aactcttccc cgctgcagta atagattacg tcgctcctgt
     3001 tcttggccac agcaacgtga tcacccttga tccagcctct gtaggtccgt gtttgtacga
     3061 agagccagga tttatcgcga cttctggcca gagcaacgaa tggactgccc aggctcaccg
     3121 aagtcaattg cagatcatcg ttgtccagaa acccctcgac caggatcagg tctgtcggca
     3181 tagccctcaa atgtgtcctg cgtgttgtga ggcccatttg aagaaccata tcctgattca
     3241 taagattggt agcgttttcc agaatatcgt cacgtatctc cgcggggacc tcttcccctt
     3301 cgggtgaaac cagcgttctt gtgaaaaagc cttccggaat cgtctctcct atcatggccc
     3361 ttatcgaatc cagctcaacc ctggtgggaa actcctccag gttacgatag aacctctcca
     3421 gaccagttct ttcggcgtta gtcagcagct cttcgttgaa agcgctcagc tcggattctt
     3481 ttagccatcg cttaccctgg cccgttattc tccgagtcca gaatttggga gaaagcattt
     3541 cgatcgaagt gccttccgga accatgaaat actgtttatg cataaacatc actcccggtt
     3601 catgtctatt tgatcgctcc cacggtaatt cctttcaaga agtatttctg gaacgcgaga
     3661 aatacgatta tcatgggaat ggctgaatat gtggcgctgg ccatgagata agaatactgc
     3721 attggcacct gttcctgcaa cagcgaaaga ccagcttgca gggttctcat ctgtgtggtg
     3781 ttggttacga tcaaaggcca tagaaaactg ttccactggc ccatgaaagt gaatatgcca
     3841 agcacggcca tcgcgggttt cgatattggc acgattatct cgatgaaagt cctgaattcg
     3901 ccggcaccgt cgattcttgc cgcttcgatt atctccgttg gaacggaaac catgaactgc
     3961 ctcatcagaa agactccgaa tggagccgcg atcgcaggca gaattagagc ctggtaagta
     4021 tctaccatcc ccatcttgga tatgagaatg tacagaggga caagcgtcac ctgagtcggt
     4081 atcatcatag aagcgatata gatggaaaat agcgctttgc tgcctgggaa acgcttcttc
     4141 gcgagagagt atccagccat ggcgcaaacg aagatctgcg ctattgtgac ggtaccggcc
     4201 actatgaagc tgttcaaggt ccatctgaag ataatgctcc tctcgaagag atctttgaag
     4261 ttctgaagcg tcgggttttt gggtatcaca tccggaggaa acttcattgt gagcgacggc
     4321 cgtgtgaaag ccgttgccac catccagtaa agtggaatga aggccagcga ggcgagtatg
     4381 gccagtatca acagcgagat tgcctttttt atattcgttt tagttttcgc tttcacggac
     4441 aatcacctca atactccaca tttgaaccca gccacttgaa ctgtatgact cccagaacga
     4501 cgagtatcac gaacatcacc atagacatcg ccgaggccag tccaaggttg taattctcga
     4561 aagccgtttc atagatgagg tacgcaatag ttgtagttga aaactcgggg cccccactgg
     4621 tcatgatgta gatgttggag aacgtctgga agttcgcaat ggtacccata accagcgcat
     4681 agaggattgt cggcttgagt aggggaaccg ttatctttct gaaaattgcc caggagctcg
     4741 ccccttcaag cgtcgcggct tcgaaatagt actcgggaat cgaggccata gccgcagaaa
     4801 gcagcactat ggtcgacccg cctcccataa tcacctgcat gattatcaaa gagggcatgg
     4861 ccgtgagtga cctgcccagc catatgacag gatccagccc taacagagtc atgagatagt
     4921 tgagaagacc aaagttggga ttaaacaccc agagccagat catcgaaatt atgacggagg
     4981 aggtcacgtg gggcaaataa aaagccgctt tgaagaaagt tcgtatcgat ttgcggaatg
     5041 gataaatcaa aacggtcacc agcaaagctt ttcccaacca caaaggcact actccgaatg
     5101 tataaacgaa ggtgtttttt aaggtcttca aaaaaagcca gtcgctcaag attcttttat
     5161 aattgtcaaa ccaaatgaat gtttgtgaga aaacattgtg tcggaacata cttaggtaca
     5221 tgccccagcc cacgggcacc agaaagaaaa ccgcaaacag agcaaaatgt ggtagaacga
     5281 agaaatatcc cgatcgattc ttccatattt cttttagcac gttcttcttc ttaagcgatt
     5341 tagtcggaat ctgcttcaaa actcatcacc ccatcaaaca ggaacagccc cagacccgac
     5401 gggggccgag gctgttcctt tg
//
LOCUS       DAOOLY010000165         5414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18172_length_5414_cov_5.170927, whole genome shotgun sequence.
ACCESSION   DAOOLY010000165 DAOOLY010000000
VERSION     DAOOLY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18172_length_5414_cov_5.170927"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLD15_08475"
     CDS             <1..624
                     /locus_tag="PLD15_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB63982.1"
                     /translation="KSIFGAHEIDSGEITINNEKTRIKGPSKAMKAGVYLCPEDRRRE
                     GVVQDMSVIDNLTSPFLGTLTRLGVISSRESNARASKVSSDLKIKTPSLKTIISHLSG
                     GNQQKVVLGKWLLGPESRIFIFDEPTQGIDVGAKSEFYRIMQEIAKKGCGVLFVSSDI
                     RELLGISDRIYVMRLGRITAEFKREEYDQHSILENALSDSLAVRSEK"
     gene            624..1565
                     /locus_tag="PLD15_08480"
     CDS             624..1565
                     /locus_tag="PLD15_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HPB63983.1"
                     /translation="MNKIGLLFQKYGTILALIGIVVFFSIMTETFLTSRNLINILSHI
                     SMLTIIATGMTVCMIPGDFDMSIASVASLSGVIVTSLILNGLGVFGSILITVGMGALF
                     GVIAGILITRLKISAFIATLALGTIATGINFMFTRGQEVYGIFPDSFLAIAQNRVLGI
                     PVQVFIMAAVIVVFTFVMEKTKMGKWMYSIGGSVKASFLSGINVRLLRVIGMIISGAL
                     AAFTGCILASRLGSGQPTAGDAYLMDAIAASFLGMTTIKVGRPNVPGTFVGALIIGVI
                     NNGLVIMGVSYFFQYIAKGAIIILAVAMTSFRNSESF"
     gene            1592..2521
                     /locus_tag="PLD15_08485"
     CDS             1592..2521
                     /locus_tag="PLD15_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB63984.1"
                     /translation="MKKVVLVLAIMLLVSLAVGVEIGFVAANLSAVTQATIAQNLEAI
                     SKTNGWTIYVSNSAGSWETMNNLVENYVSKKVDLIVVAMGQASSLTSSLTAAKEAGIP
                     VIGIDSEYSDLLTADILTNNWEMGAKIATYLVDRLNHKGNIIVFKFDQFYGTRFRGKA
                     LDVVLTEEPNIKVLEVHHLPPAGFVEDAQRTMESYLLKYGDKIDAVWCAWDDPAYAVS
                     LAIRAAGYTQEDMFVVGIDGNERNIGMIAANNTAVVATILQPFGDSATRAAELIQKIV
                     VEGNSPEEVIGAVRVIYMDAPLITSANAQDYLK"
     gene            2646..4166
                     /locus_tag="PLD15_08490"
     CDS             2646..4166
                     /locus_tag="PLD15_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB63985.1"
                     /translation="MIHVHDLEREGVSAVKERELFAGYDIGTTSSKISITDNEGRIVA
                     SAKVNHSQSFSDDGYPEQDARVWWQDFLKLTAMAGKEIPLSSIKAIGLSSMCPNVLPV
                     SGDGTPLRKSFMYGIDQRAAKEIELLNEFMRQAGDNPLYTEYSSQSILPKILWLRKNE
                     PQIFERTARVLTANGYIAFRLTGQFASDIFSLSAGNLIDLNTLEIFEDPFDHADLPTE
                     IIPDIKWATDLTGKVTRRASEETGLVCGTPVFTGTGDACSDAITNACLEPGNVSISLG
                     GTSIYIQCLDRPVRSPALFVETGAVPGSFTAGGATSCGGLLTDWVTDRLFGLDMDEKE
                     SLLSGFRAEEYEPSNMSLLPFFGGARTPFNNPKARGILFGLSMETGRKEILSSVFEAI
                     ALDISMIGDEIKRTALEPNQICVSGGGARSEVLLQLIATVLDHELLVSPRQYDSATGA
                     ALIALSATSGKALEKVVEEIKKKYDRVEPRAEYSSYMKERKNIFKELYTANIGLFR"
     gene            4767..>5414
                     /locus_tag="PLD15_08495"
     CDS             4767..>5414
                     /locus_tag="PLD15_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63986.1"
                     /translation="MKRVPLLMLLLFIMLLSTAFPVEVFTRRQLLEDIDFLVGKIESL
                     FYDPWRKIDEEVFRDLVEEAKSSLKDGMTLSEFFLVAGRITDSLKDQHSGMSFPGEVN
                     VLPFKTREVRGRALVIESESSIPVGSFILEIEGFTIDELYEKYGSSMGYHESEETGPN
                     IFINWFIHAIPQFMNSPEVEVTYLFNDEIRKAAINSISVSDSDWQMMRNSNRSPAF"
BASE COUNT         1434 a         1361 c         1388 g         1231 t
ORIGIN      
        1 aaatctatct tcggtgccca cgaaatcgac agcggcgaaa taaccataaa caacgaaaaa
       61 accaggataa aaggcccttc gaaagctatg aaggccggtg tatatctctg cccggaggac
      121 agaaggaggg agggggttgt ccaggatatg tcggttatag acaacctgac ctctccattc
      181 cttgggacgc tgaccaggtt aggtgtgatc agctcgagag agtccaacgc cagggccagc
      241 aaagtatcga gcgatctgaa gataaagacg ccgtcgctga aaacgataat ctctcatctg
      301 agcggcggca accagcagaa ggtggttctg gggaaatggc ttctgggacc ggaatccagg
      361 atcttcatat tcgatgagcc gacccaggga atagatgtcg gagccaagag cgagttttac
      421 aggataatgc aagaaatcgc taagaagggt tgcggggtac tgttcgtctc ctcggatatc
      481 cgggagctgc tgggtatatc cgacaggatc tatgtcatgc gcctggggag gatcacggcg
      541 gaattcaaaa gagaggagta cgatcaacac tcgattctcg aaaatgcgct cagcgacagc
      601 ctcgccgtaa ggagtgaaaa ataatgaata agatagggtt gctctttcag aagtacggca
      661 ctattctggc cttgatcggg atagtcgtct tcttctcgat aatgaccgaa acgtttctca
      721 cttccagaaa tctcataaac atcctctccc atatttccat gctgacgata atagcgaccg
      781 ggatgaccgt ctgcatgata ccgggagatt tcgacatgtc tatcgcttcg gtggcgagcc
      841 tttcgggagt tatagtgacc tctctgatct tgaacggcct gggcgtgttc ggttccatac
      901 tcatcacggt aggcatgggc gcgctcttcg gcgtgatcgc cggaatcctg atcaccagat
      961 tgaagatctc cgccttcatc gcgaccctcg ccctcggtac gatcgcgacc ggtatcaact
     1021 tcatgttcac cagaggccag gaagtttacg ggatcttccc cgattccttc ctcgcgatcg
     1081 cgcagaacag agtcctggga atccccgtcc aggttttcat aatggcagcc gtcatagtcg
     1141 tcttcacctt cgtgatggaa aagacgaaga tgggaaagtg gatgtactct atagggggca
     1201 gtgtgaaggc ctcttttttg tcgggcatca acgtcaggct tctccgcgtg atcggaatga
     1261 taatctccgg cgccctcgcg gccttcaccg gctgtattct cgcctccagg ctgggatcgg
     1321 gacagccgac cgcgggcgac gcttacctca tggatgcgat agccgcctcc ttcctgggaa
     1381 tgaccacgat caaggtgggc aggccgaacg taccggggac tttcgtggga gctttgatca
     1441 tcggagttat aaacaacggt cttgtaataa tgggagtttc gtatttcttt cagtacatcg
     1501 ccaaaggagc gatcataatt ctggccgtcg cgatgacctc attcagaaac tctgagagct
     1561 tctaaggcaa acaacaggag gtgtggtaac tatgaaaaag gtagttcttg ttcttgcgat
     1621 tatgttactc gtttcgctgg ccgtcggtgt cgagatcggc ttcgtcgcgg ccaacctttc
     1681 tgcggtaacc caggcaacga tcgcccaaaa tctcgaagcc atttcaaaga ccaacggctg
     1741 gaccatctac gtctcgaact ctgcaggctc ctgggagacg atgaacaacc tggtggaaaa
     1801 ctacgtgtcg aagaaagtag accttattgt ggtcgccatg ggtcaggcaa gctcgctcac
     1861 ttcctcgctc accgcggcga aagaggcggg catccccgtg atcgggatcg attccgagta
     1921 cagcgacctg ctgacggccg acattctcac caacaactgg gagatgggtg caaagatcgc
     1981 cacctatctg gtcgacagac tgaaccacaa gggaaatata atcgtgttca aattcgatca
     2041 attctacggg acccgcttca gaggcaaggc cctggatgtt gtgttgactg aagagccgaa
     2101 catcaaagtg ctcgaagtcc atcacctgcc gccggcggga ttcgtcgaag acgcccagag
     2161 aaccatggaa tcgtacctgc tcaagtacgg cgacaagatc gacgccgtct ggtgcgcctg
     2221 ggacgacccg gcttacgcgg tatccctggc tataagggct gccggctaca cccaggaaga
     2281 catgttcgtg gtcggaattg acggcaacga aagaaacata ggaatgatag cggccaacaa
     2341 cacggccgtc gtagccacga tcctgcagcc cttcggagac tccgccacca gggcggccga
     2401 gctcatacag aagatagtcg tcgagggaaa cagccccgaa gaggtcatcg gagccgtccg
     2461 ggtaatatac atggacgccc cgcttataac ctcggcaaac gctcaggatt atctgaaata
     2521 accaactgaa agaagtactg aagcgctttc gggagcctgt tgcattcacc tgtcgagagt
     2581 cgaggggtag atacgattcg aggatttttt taatgcaaag gctcccccgt ttttcttcat
     2641 tacccgtgat tcacgtacac gatctagaaa gggagggtgt atcggccgtg aaagaaagag
     2701 agcttttcgc aggatacgat atcggtacga cttctagcaa aatatccatc acagacaatg
     2761 aaggccgtat tgtcgcaagc gcaaaggtca atcattcaca gagtttttcc gacgatggct
     2821 atcccgagca agacgcccgg gtgtggtggc aggactttct gaaactgact gcgatggcgg
     2881 gcaaagagat ccccctctcg agcataaaag cgataggact cagctccatg tgtccgaacg
     2941 tgttgcctgt atcgggcgat ggaactcccc tgcgaaagtc tttcatgtac ggaatcgatc
     3001 agagggccgc taaagagatc gaactgctca acgagttcat gagacaggcc ggagacaatc
     3061 cgctgtacac agagtattca agccagtcca tccttccaaa gatcctctgg ctgagaaaaa
     3121 acgaaccgca gatattcgaa aggacggccc gggttcttac cgcgaacggt tacatagcct
     3181 tcagattgac cggccaattt gcctcggata tcttctcgct ttcagcgggc aacctcatag
     3241 acctcaacac cctggagatt ttcgaggatc ccttcgatca cgcagacctc ccgacagaga
     3301 taattccaga tataaaatgg gctaccgacc tcaccggcaa ggtaacgaga cgggcaagcg
     3361 aagaaactgg gctggtttgt ggaactcccg ttttcaccgg aacgggagac gcatgctccg
     3421 acgccataac caacgcctgc ctcgagccgg gaaacgtctc catctcgctg ggagggacct
     3481 cgatttacat ccagtgtctc gacagacccg taagatcgcc cgccctattc gttgaaaccg
     3541 gtgccgtccc gggatccttc accgccggtg gcgctaccag ctgcggcggc ctcctcaccg
     3601 actgggtgac cgacagactg ttcggcctcg acatggacga gaaagagagt ctgctgtcgg
     3661 gttttagagc ggaggaatac gaaccttcaa acatgagcct tcttccgttc tttggaggag
     3721 cgcgcacccc cttcaacaac cccaaagcca gaggaattct cttcggactg tccatggaaa
     3781 ccggcagaaa agagatcttg agttcggttt tcgaagccat tgccctggac ataagcatga
     3841 taggggacga gataaagaga actgcgctcg aaccgaacca gatctgcgtc tcgggcggcg
     3901 gtgccaggag cgaagttctg ctacagctga tcgccaccgt gctcgatcac gagctgctgg
     3961 tctcgccccg ccagtacgac tcggcgacag gtgccgccct gatcgctctg tcggccacga
     4021 gcggtaaggc tctcgaaaaa gtcgttgaag agataaagaa aaagtacgac cgggtggagc
     4081 ccagagccga atacagttcg tacatgaaag agagaaaaaa tatctttaaa gaactctaca
     4141 cggcaaacat cggccttttc agataataaa taaatctgtg ccggtctcca aacatatttc
     4201 taaaactaac gaagtgagag ttaaaaagca caccatagct caaacctttt caaggccagt
     4261 ttttctaaat gaaggcaaaa ggactcagaa actatttctc atctttcttt gtgctctgcc
     4321 aagtataaga taatagatta ctgacatgac tgtgacaaca tatacacctg cgaccaggct
     4381 ccacaatctt gggttttgga ttagcatgtc gtatcgtgtt acaacgcagt atgttgtgta
     4441 aatacttgca acgatcattg cgatagtcaa cacacctaca gagaacttca agctcttgag
     4501 ctcggctttt cttcttggta cgagcctcat tttactaata tccccctatt acccacagaa
     4561 tcaaaatcac tcaatctata gttagattag cgaaattaat tataacaaat aagtatttaa
     4621 aaaatcaaat gatcgtctat acttttattt gaaaagtaaa tatatgaatt gttaggtaat
     4681 caataaaaaa tcaaactaaa tcactgcgat ttctcttagc aggaatttgt aagtccatag
     4741 catataaata taaggagaga ttcctaatga agagagtgcc tttgcttatg cttttattgt
     4801 ttatcatgct cctttcaact gcctttccgg tagaggtttt cacccgcagg cagcttctgg
     4861 aagatatcga ttttctcgtc gggaaaattg aaagtctttt ctacgatccc tggcggaaga
     4921 tagatgagga ggttttccgc gatctcgtgg aagaggcgaa gtcgtcactg aaagatggga
     4981 tgaccctgag cgagttcttt ctggtcgcgg ggaggattac cgatagcctg aaggatcagc
     5041 actcgggaat gtctttcccc ggagaggtca atgtactgcc tttcaaaacc agggaagtac
     5101 ggggcagggc gctggtgatt gaatctgaga gttcaatacc cgtcgggagc tttatccttg
     5161 agatagaagg attcacaatc gacgagctct acgagaaata cggcagctcg atgggttacc
     5221 acgagagcga ggagaccgga cctaacatct tcattaactg gttcattcat gcaatcccgc
     5281 agtttatgaa cagccccgaa gtggaggtca cttacctttt caacgatgaa atacgcaaag
     5341 cggccatcaa ttccatttca gtgagcgatt cagactggca gatgatgaga aactcaaaca
     5401 gatcaccggc cttc
//
LOCUS       DAOOLY010000166         5407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18204_length_5407_cov_4.349776, whole genome shotgun sequence.
ACCESSION   DAOOLY010000166 DAOOLY010000000
VERSION     DAOOLY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18204_length_5407_cov_4.349776"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLD15_08500"
     CDS             complement(<1..401)
                     /locus_tag="PLD15_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB63987.1"
                     /translation="MLKVANLNVYYGGIHALKGISFNVEEGKIVALIGANGAGKSTTL
                     RSICGLKKVREGEITFKSEAITNKPTNDIVKSGMTLVPEGRRIFPNLTVTENLMLGAF
                     LRNDGDEIAKDLEWVNTLFPRLKERKDQKGGT"
     gene            complement(394..1167)
                     /locus_tag="PLD15_08505"
     CDS             complement(394..1167)
                     /locus_tag="PLD15_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63988.1"
                     /translation="MSLLKLEHVTKRFGGLTAVSDFNLDLQEGELVGLIGPNGAGKTT
                     IFNLITNTYLVSEGKVILGGKDITGMKTDRITAEGIARTFQNIRLFGELTALDNVRTA
                     FHLRLKSSVLSAVFGLPGYRKEEKEIDDKAMLLLKTLGLEKYKDDLAGSLPYGLQRKL
                     EISRALATNPKVLLLDEPAAGMNPEETLALAALIMRIRKEFNLTVLLIEHDMSLVMSI
                     CERIVVLDHGVTIARGLPKEIQNNPEVITAYLGTGDLYA"
     gene            complement(1164..2189)
                     /locus_tag="PLD15_08510"
     CDS             complement(1164..2189)
                     /locus_tag="PLD15_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB63989.1"
                     /translation="MKLILTIVSLLVFILLLWLAESYMDPFIKRILNVAAIYVILGVS
                     LNLINGFTGQFSLGHAGFMSLGAYASALLYMSPALKATNFFIEPIIWPLSEIQIPFFF
                     ALLVGGAIAAVAGFAVGAPCLRVGGDYLAIVTYGFAEIIRVILNNLQNITNGPLGLKG
                     LPTYTNLYWSWGWALFTIFFIKKLVDSSYGRALKSIREDEVAAEAMGVNLFKHKTLAF
                     VIGAFFAGIGGGLLGSLLMTIDPNSFNISLTFQIVMIVLLGGLGSITGSVVMGVSIAF
                     LMELLRFVESPMTVFGLTIPGISGMRMLIFALILVITVIFFRRGLFGQKEFSWDWIFR
                     SKRRNAE"
     gene            complement(2207..3094)
                     /locus_tag="PLD15_08515"
     CDS             complement(2207..3094)
                     /locus_tag="PLD15_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB63990.1"
                     /translation="MSPDVFLQHLVNGISLGFIFGLIAIGYTLVYGVVKLVNFAHGDV
                     FMMATFFVFYGFTLFMMPWWLAVIFGIVVTILLGVGIERIAYRPLRDAPRISSLCAAI
                     GMSFLIQNIATVVFGGRQKSFYVPPSLTTTFVIGGVRIQAITIATIVVSVIVLLLLTF
                     LIKRTKMGLAMRALSVDFDTAKLMGINVNRTIAFTFAIGSALAALSAILWALRYPQIW
                     PFMGVFPGWRAFTAAIIGGIGSIVGALFGGFMLGMVTILLVAFFPEAAGYRDAIIFVL
                     LVVILLVKPTGLFGEKKSR"
     gene            complement(3195..4322)
                     /locus_tag="PLD15_08520"
     CDS             complement(3195..4322)
                     /locus_tag="PLD15_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB63991.1"
                     /translation="MKRILVLAVVLLLGFVLFAVDPIVVGVFEPMTGPYAAGGQLTME
                     GITLAAEQVKEILGRPVELVLVDNKSEKVEASNAVSRLIQFNKASVIIGSYGSAVAIP
                     GSEVANAAGVPMIGCSPTNPLVTVGKPYAFRVCFIDPFQGSVMAKFAVEELKAKTAVI
                     IQDIASDYSVGLSHYFQNSFKSLTGDNKSISGVISYQTGDQDFTAQLAYAQGKKPDVI
                     FIPAASYGEAALIIKQARELGIQAQFLGGDTWEVPEFLQVGGAAVEGSFFSTHFDVAV
                     APTPKAAGFIEAFKAKYGVEPSAFAALGYDAFMLAIDAIVRANSAVPEDVKNALSATV
                     NFEGVTGYITIDENGDAVKDAIVRKVENGAFKFVSVVKPAQ"
     gene            5013..>5407
                     /locus_tag="PLD15_08525"
     CDS             5013..>5407
                     /locus_tag="PLD15_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB63992.1"
                     /translation="MESETMIAIVKERPGPGLTLKRVPVPGEPGPHDVLVKVKRASIC
                     GTDLHIYKWDEWSQKRIRPPQIGGHEFTGEVIKVGDNVTTVAVGDSVVAETHIPCQKC
                     LQCKTGRMHICRDMEILGVHRDGVFAEYVL"
BASE COUNT         1438 a         1391 c         1244 g         1334 t
ORIGIN      
        1 gtgccgcctt tctgatcctt tctctctttc aaccttggaa aaagcgtgtt gacccactcg
       61 agatccttgg ctatctcgtc gccatcgttc ctcaaaaaag ctccgagcat gagattttcg
      121 gttaccgtga ggtttggaaa tatccttctt ccctccggaa ctagggtcat tccggatttc
      181 acgatatcgt tcgtcggttt gttggttata gcctcggatt tgaaggttat ttctccttct
      241 ctgacttttt tgaggccgca tatgctcctc agcgtagtgg acttcccggc tccgttggct
      301 cctataagcg caacgatctt gccttcctca acgttgaagg aaatgccctt taaggcgtga
      361 atgcctccgt agtaaacgtt caggtttgcg actttaagca tacaaatcac ccgttccaag
      421 gtaagctgtg ataacttcag gattgttttg aatctctttg ggtagaccgc gcgcgatagt
      481 cacaccgtga tcgagtacca ctatcctctc acagatgctc ataacaagac tcatatcgtg
      541 ttctatcaac aaaaccgtta agttgaattc ctttcttatc ctcattataa gggccgccag
      601 cgccagcgtc tcctcggggt tcattcccgc agcgggctcg tccagcagca aaactttcgg
      661 gttagtcgcg agggccctgg atatctcgag ctttctctgt agcccgtagg gaagcgaacc
      721 ggcaagatcg tccttgtact tctccagacc cagtgttttc agcaggagca tggctttgtc
      781 gtcgatctcc ttttcctctt tcctgtagcc tggtaatccg aaaacggcgg acagtacaga
      841 ggatttaaga cgcaggtgaa aggccgtcct cacgttgtca agcgccgtga gttcgccaaa
      901 gagacgtatg ttctggaagg ttctagctat gccctcggca gttatcctgt cggtcttcat
      961 tcccgttata tcttttccgc cgaggataac cttgccttcg ctcaccagat aagtgttcgt
     1021 tatgaggttg aatatcgtag tctttccggc tccgttcggt cctatgagac ctacgagttc
     1081 accttcctga agatcgaggt tgaagtccga gactgccgtg agcccgccga atctcttggt
     1141 cacgtgttca agtttaagca gactcattct gcattcctcc tttttgatct gaagatccag
     1201 tcccaagaga actccttctg accgaagagg cctcgcctga agaatataac ggtaataacc
     1261 agtatgagcg cgaatatcag cattctcatg ccagaaattc ccggtatcgt caggccgaat
     1321 acagtcatcg gtgactcaac aaacctcaag agctccatca ggaaggcaat cgatacgccc
     1381 ataacaacac taccggttat gcttcccaga cctccaagca acacgatcat aactatctgg
     1441 aaagtcagag agatgttgaa agagttcgga tcgatcgtca tgagaagact tcccagcaat
     1501 cctccgccga tgcccgcaaa aaacgctcca ataacgaaag ccagagtttt atgtttgaag
     1561 agattgacgc ccatcgcttc agccgcaacc tcgtcctctc ttatcgattt cagagctctt
     1621 ccatagctgc tatcgaccag tttctttatg aagaaaatcg tgaacagggc ccagccccag
     1681 ctccagtaga gattggtata agtcgggagc cctttcaaac cgagcggacc gtttgtgatg
     1741 ttctgaaggt tattcagtat tacacggatt atctcggcaa aaccgtaggt aacgatcgcc
     1801 agataatctc ctcccactct gagacatggc gcaccgaccg caaatcccgc aaccgctgcg
     1861 atggctcctc caactaacaa cgcaaagaag aaaggtatct gtatctcgct gagcggccag
     1921 atgatgggct ctatgaagaa attggtggcc ttcagcgccg gagacatata aagcagagcg
     1981 gatgcataag ctcccagaga cataaacccg gcatgtccga gagagaactg acccgtgaaa
     2041 ccgtttataa ggttaagact gacacccaga attacataga tagccgccac gttcagaatc
     2101 cttttgatga aaggatccat atagctttca gcgagccaga gcaacaaaat gaagaccaga
     2161 agactcacta ttgttaagat taacttcatt ctgcgatcca caacagtcac ctacttttct
     2221 tctcgccaaa caggccggtc ggcttgacga gcagtattac aacgagaagg acgaatatta
     2281 tcgcgtctct gtaacctgcc gcttccggga aaaatgccac aagcaagatc gtcaccatgc
     2341 ccagcatgaa tccgccgaac agagcaccta cgatgcttcc tataccgccg attatcgctg
     2401 ccgtaaaggc tctccatccc ggaaagacgc ccatgaaggg ccatatctgc ggatatctga
     2461 gtgcccagag tatagcgctg agggccgcaa gcgccgaacc gatagcaaat gtgaacgcga
     2521 tcgttctatt tacatttatg cccattagtt ttgccgtatc gaaatcgacc gagagagctc
     2581 tcatggccag tcccatcttt gtacgcttta taaggaaagt aagtaacaga agcacaatta
     2641 cggaaactac tatcgtggct atagtgatcg cctgaattct tacgcctccg atcacgaaag
     2701 tggtggtcag tgaaggaggc acatagaagg atttctgtct tcccccgaat acaaccgtcg
     2761 caatgttttg aatcaaaaac gacataccta tggccgcaca gagagaggat atcctcggcg
     2821 catctctgag aggtctatag gctattcttt caataccaac tccaagcaat atcgttacca
     2881 caattccaaa tataacagca agccaccaag gcatcataaa caaagtaaaa ccatagaaaa
     2941 cgaagaacgt agccatcatg aacacgtcgc cgtgcgcgaa attcacaagc ttgaccactc
     3001 cgtagacaag agtgtaccct atggctataa gtccgaatat gaagcccaaa gaaatcccgt
     3061 taacaaggtg ttgcaaaaat acatcagggc tcatcctgcc accgccttta ctttaactac
     3121 tacaattcag tatagacgca ggcacaggcc tgcgtccaat acctatgctt cgaaacagct
     3181 gccttcagtg agatttattg ggcaggcttg acaacactga caaacttgaa cgcgccgttt
     3241 tcaactttcc tgactattgc atcctttacg gcgtctccat tttcgtcgat cgttatgtaa
     3301 ccggtcacgc cttcgaaatt cacagttgcg ctgagggcat tcttgacatc ctcaggaact
     3361 gcagagtttg cccttacgat cgcatcgata gccagcatga atgcatcgta tccgagcgct
     3421 gcgaacgcac ttggctcaac gccgtacttc gccttgaagg cttcgatgaa tccggccgcc
     3481 ttcggagtcg gtgcgactgc aacgtcgaag tgagtgctga agaagcttcc ttcaacggcg
     3541 gctccgccga cctgcaggaa ctccggtact tcccaggtgt ctccaccgag gaactgcgcc
     3601 tgaattccca gctcacgtgc ctgcttgatg atcagtgcag cttcaccgta agatgcggcg
     3661 gggatgaaga ttacgtcggg tttctttccc tgagcgtatg cgagctgcgc tgtgaagtcc
     3721 tggtcaccgg tctggtaaga gatgacaccg cttatcgatt tgttgtctcc cgtaaggctc
     3781 ttgaaggagt tctggaagta gtgcgaaaga cctaccgagt agtcggaagc gatatcctga
     3841 ataatgacag cggtctttgc cttcagttct tcaactgcga atttggccat aacgcttccc
     3901 tggaacgggt cgatgaagca gactctgaaa gcgtagggct tgccaactgt cacgagcggg
     3961 ttggttggcg aacatccgat catcgggact cctgccgcgt tggcgacttc gctacccggt
     4021 atcgccacgg cgcttccgta actaccgatg attacagatg ccttgttgaa ctgaatcaat
     4081 ctggaaactg cgttggaagc ttcgactttc tcactcttgt tgtcgaccaa aaccagttct
     4141 accggacggc cgaggatttc cttcacttgc tcggctgcaa gggtaattcc ctccatagta
     4201 agctggccac cagctgcgta aggccctgtc atgggttcaa aaacaccaac gacgattggg
     4261 tcaactgcga acaacacaaa acctaacagc agaacaactg ccagaacgag aatcctcttc
     4321 acttacctca cctccacaat tgtgatcgtc tacgatcgtc taaggctgaa attaccccac
     4381 taaatataga tggtactcaa aggcacggtt gtaagtgtta gcggacaact gtgcaaaata
     4441 taaaaacttt cgggtaagaa acaagaaaca cctggcgaca tttcttaaga caactacttc
     4501 agactctgag ttcactagtt acttaacttt caattttaga caaccaaatc ggccattcaa
     4561 tatggtaacc accaaacaat ctattatcat gataccacgt gagcactcta gaatctctct
     4621 ccaaaagtcg cccttttgag tcgatacaag cgatcatgga cgattatcga gttaaatcgt
     4681 ttggaaaacg aagaataagt gagcgggagg taagaaacgt taagcgtacg aattttgtaa
     4741 gatcgagtat tgaggagtat gcttttaatg atagcacatg aaagaacctt ccagtcttta
     4801 ataattatga ctacccgggt gatttacagt tacctgtttg ataactggaa cacttcgtga
     4861 gtcgtacggt acgttaccgg gagtattgaa caactggaga gctgtcagta ttgacagttg
     4921 cccatagatg cccggcggtg aggtttttta agttcgacgt ttaacttaca attggagtat
     4981 ggcggctaac aaccagaagg agggaaattg cattggaatc agaaactatg atcgcaatag
     5041 taaaggaaag accgggtccc ggactgacat tgaagagggt tcccgtaccg ggagaacccg
     5101 gcccacacga tgttctggta aaggtcaagc gcgcttcgat atgcggcacc gatcttcaca
     5161 tctacaaatg ggatgaatgg tcccagaaaa ggatacgccc tccacagata ggcggccacg
     5221 aattcaccgg agaggtaata aaggttggcg acaatgtcac gaccgtcgcc gttggagatt
     5281 ccgtggttgc cgaaacgcac atcccctgtc agaaatgtct tcagtgcaag accggtagaa
     5341 tgcatatttg cagagatatg gagatactcg gcgttcatcg agacggggtc tttgcagaat
     5401 acgttct
//
LOCUS       DAOOLY010000167         5382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18303_length_5382_cov_3.754083, whole genome shotgun sequence.
ACCESSION   DAOOLY010000167 DAOOLY010000000
VERSION     DAOOLY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18303_length_5382_cov_3.754083"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLD15_08530"
     CDS             <1..1153
                     /locus_tag="PLD15_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB63993.1"
                     /translation="QKNKALIAYIGTGSLGVAWYKRGRLYSTQNIKLGSLKLTELLGE
                     LQSKTNRFHQIVEDYLSSFKEMIPKSSPFGKIDHFVVCGQEIEIIASLCATSAGTEIT
                     QIPIAKLDELYDNLKSKTPSEVAQMYGIDEEMAEILLPSLGIYRLLASFTRSKYIYAS
                     RVTLQDALLKEILLTREAKKRLESFREDIIQCARTLGKRFFFDEEHGNRVVRNAMAIF
                     QKLKTPYGLDENDALILKVASLVHDIGKYVNFTEHYRRSYELIRGSNLPGVSQADLET
                     IALISLFHSNLVPSESDPALGLLSMEQRVRVSKLAGILRLADALDRSHSEKIDVMKVR
                     MKDNEISLTIHSREDILLEEWAFNSKARLFEEAFGLKVNLKKKVNPVES"
     gene            1143..3254
                     /locus_tag="PLD15_08535"
     CDS             1143..3254
                     /locus_tag="PLD15_08535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HPB63994.1"
                     /translation="MNLDDPNLYLNRELSWLDFNERVLEEAFDKENPVLERLKFLSIT
                     SSNLDEFFMIRVAGLKEQEEAGYAGRDPSGMTATEQLKAISIKVHELVERQNNCLHRS
                     LLPALKKENIFFLKLDEFDGEQKEYIERYFIDTVYPVVTPMAIDQSRPFPFLQNRSIN
                     LGVVLEQEEQPLFAVVQIPPVLPRLIKLPSKRGECFTFMGDILKSHMNRLFSGYHIRD
                     IHAFRITRNADLSIEEEDAHDLLIEIEKSLQRRRWGFPVRLEIERGMNVELREYLKEM
                     LELADEDIYVSGPPLDLTLWMKFLQLKAFDNLRYPKALPQASPDLYGQDDLFKIIRDE
                     DTMLHHPFESFQHISDLVYKAAEDPRVLAIKQTLYRVSGNSPIIDALVHAADNGKQVT
                     VLVEIKARFDEESNIQWAKTLERAGCHVVYGLVGLKTHVKMLLVVREEDDGIRRYLHL
                     STGNYNDTTARLYTDIGLLTVRDQLTTDASALFNVLTGYSLPPRWKAFAVAPYNLRET
                     FLRLVQSEIDNASRGYEARIIIKINSLLDKECIKKLYEASIAGVRIDLIVRGICCLKS
                     GIEGVSDNIRVISVVGRYLEHSRIFYFQNRDNPKFFLSSADLMPRNLDRRIEVMFPVE
                     DERLRKRLKSILEIVLKDTVKARLQFPDGKYKKVDRRGKPLVNSQEMLHKAAISRLRK
                     FEKERISELASNPVLRSREKA"
     gene            3422..4600
                     /locus_tag="PLD15_08540"
     CDS             3422..4600
                     /locus_tag="PLD15_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB63995.1"
                     /translation="MISRKLDSENIGKSNRRLVLQLLRKNLITTRRDLASVSGLNPST
                     VTKIIREFLLAGLCEEISTEVTDRIGRKAIELRLNRKAYVSIVVDIGVEETSIGKGYF
                     DGTTSTISSFSTPSTFEEFTEVLVEKTGEVVKKIPKYKFLGYSISVPGMVDVERSKIV
                     YVPHLGWKDVFLKDSLSKRYPVVLDNEANLSLIAEKWNNPGLAGLGDIVFVYVSEGIG
                     CGIMLGGQIHRGRDYSAGEIGHMTVQLDGPKCYCGNSGCWETFASSEAIVRKAQSEGL
                     ELKGSNNNERYLDILKNYEKSEYSFLIEEIEKSLAVGIVNIVNSLDPEVIVLGGVASM
                     LPEEALKRLTGIVNSRVLYATGQNIEVRRSILDTHGKTSSNMIGAALHIIERRVDDFI
                     "
     gene            4612..>5382
                     /locus_tag="PLD15_08545"
     CDS             4612..>5382
                     /locus_tag="PLD15_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPB63996.1"
                     /translation="MEVFVGIDIGTTSVKAIAVDSKGKIQKSLSRSLPLHTPKPGWVQ
                     QNPKDWWQAVVEVLSELSKSGCRIAAISVSGQMHSLVALDDRDEPVYPSILWCDQRTE
                     KQCEELTGKLGGERAVIESFGNPILTGFTLPKFLWLSENEPERFSTIIKWMLPKDYVA
                     YRLTGRSTIDYSDASGTSALTLEGEFSTRALELCSINRSSNPELVSSGEVIGSVLEGT
                     IPGLEGIPVVAGGADNASAAFGCGVEWPGDTMVSLGTSG"
BASE COUNT         1576 a         1209 c         1355 g         1242 t
ORIGIN      
        1 gcaaaagaac aaagctttga tcgcctacat aggcacgggc agtctcggag tggcctggta
       61 caaaagaggg aggctgtaca gcactcagaa tataaaactg ggatctttga aattgacgga
      121 actgctcggg gagcttcaat cgaaaaccaa caggttccac cagatagtcg aggattatct
      181 gagttccttt aaggaaatga tacccaaatc ctcgcctttc ggaaagatcg accatttcgt
      241 tgtatgcggc caggaaatag agatcatcgc ttccttatgt gcaacctctg ccggtacgga
      301 gatcactcaa attccgattg cgaagctgga cgaactgtac gataacctca agagtaagac
      361 accatcggag gttgcccaga tgtatggaat tgacgaagaa atggcggaaa tactcctccc
      421 ttctctggga atctacaggc ttcttgccag tttcaccaga agtaagtaca tatacgccag
      481 ccgtgtcacc cttcaggatg ccctgctgaa ggagattctt ctgaccagag aggctaaaaa
      541 acgtctcgaa agcttcagag aagatataat acagtgtgct aggaccctcg ggaaacgttt
      601 tttcttcgac gaagaacacg gaaacagagt ggtcaggaac gctatggcga tcttccagaa
      661 attaaaaaca ccttacggac tggacgaaaa cgatgcgctg attcttaaag ttgcttcact
      721 cgttcacgat ataggcaaat acgttaattt cacagaacat tacagaagat cctacgaact
      781 cataaggggt tcgaatctgc cgggtgttag tcaagcggat ctggaaacaa tagccctgat
      841 ctctcttttt cacagtaatc tcgttccatc ggaatcggac cccgcattag gacttctttc
      901 catggagcag agagttcggg tttccaaact ggcaggtata ttgaggctcg ccgatgcgct
      961 cgacagatcc cactcggaaa aaatagatgt tatgaaggta aggatgaagg ataacgagat
     1021 ttccttaact attcactcga gagaggacat tcttctagaa gagtgggcct tcaacagcaa
     1081 ggccagactt ttcgaagagg cttttggcct gaaagttaac ctcaaaaaga aggtgaatcc
     1141 cgttgaatct tgacgatccc aatctgtacc tgaacaggga actcagctgg cttgatttca
     1201 acgaaagagt tctggaggag gctttcgaca aggaaaatcc ggttttagaa aggctgaaat
     1261 ttctttcgat cacatcctct aacctcgacg aattttttat gataagagta gccggtctca
     1321 aagaacagga agaggccggg tatgcaggta gagacccatc aggtatgacc gcgactgaac
     1381 agctcaaggc catttcaatc aaggttcacg aattggttga acgtcaaaac aactgccttc
     1441 acagatcgct actgccggcg ttgaaaaaag aaaatatctt ctttctcaaa ctcgacgaat
     1501 ttgacggtga gcagaaagaa tatatcgaga ggtacttcat agataccgta tatcccgttg
     1561 tcacaccgat ggcgatcgat cagtcgagac ccttcccttt tctccagaac agaagcatca
     1621 atcttggggt ggtactggag caggaggagc aaccgctctt tgccgtggta cagatacctc
     1681 ccgttcttcc tagattgatc aagctgcctt cgaagagggg agagtgtttc actttcatgg
     1741 gagatatctt gaaatcccat atgaaccggc tcttctcggg ataccatatc agagatatcc
     1801 atgccttcag gatcacacga aacgccgact tgagtatcga agaggaagat gcccacgatt
     1861 tgctgatcga gatagagaaa tcccttcaaa gacgtagatg gggctttccg gtgaggctag
     1921 agatcgaaag ggggatgaac gttgaattga gagagtacct gaaagagatg cttgagctgg
     1981 cagatgagga tatatacgtt tcgggaccgc ctctcgatct cacgctgtgg atgaaattcc
     2041 ttcaactgaa agctttcgat aacctcagat atccaaaggc acttccgcag gcttcgcccg
     2101 acctttacgg tcaggacgat ctcttcaaga tcataaggga cgaagataca atgcttcacc
     2161 accctttcga atcatttcaa catatatctg atctggttta caaagcggcc gaagaccccc
     2221 gtgtactggc cataaagcag acgctctaca gggttagcgg aaattctccg atcatcgatg
     2281 cgctggtgca tgcggccgat aatggaaaac aagttacggt actcgtggag atcaaggcca
     2341 ggttcgacga agagagcaac atacagtggg ccaaaacgct cgaaagagcg ggctgccacg
     2401 tggtgtacgg actggtgggg ttgaagacac acgtgaaaat gctactggta gtgagggaag
     2461 aagacgacgg tataaggcgt taccttcatc tgagcacggg aaattacaac gatacgacag
     2521 caaggctata cacggacatt ggactgctga cggtgagaga tcaactaaca accgatgctt
     2581 cagcactttt caacgtactc acgggttact ctttgcctcc aagatggaaa gccttcgccg
     2641 tggctcctta caacctacgc gaaactttcc tgagactcgt tcaatcagag atagacaacg
     2701 cctccagggg ttacgaagcc cgcataatca tcaagatcaa ctccctgctg gataaagaat
     2761 gcataaaaaa actctatgaa gcttcgatcg ccggtgtcag gatcgatctt atagttcggg
     2821 gcatctgctg cctcaagtca ggcatcgagg gcgtcagcga caatataaga gtcataagcg
     2881 ttgtaggcag ataccttgaa cacagccgga tcttctactt tcagaacaga gacaacccca
     2941 agttctttct ttccagtgcc gatctcatgc caagaaacct cgacagaaga atagaggtga
     3001 tgtttcctgt agaggacgag aggctcagga agaggctgaa atcaatactg gagattgtgt
     3061 tgaaagatac tgtaaaggcc agactccagt ttcctgacgg gaaatacaaa aaagttgaca
     3121 gaaggggtaa accactggtg aattctcaag agatgctgca taaagccgcg atatccaggc
     3181 tcaggaagtt cgaaaaagaa agaatctctg agctggccag caacccggtg ttgagatcga
     3241 gagaaaaagc atgaaaatga acgaacgtcg gaaagggtct cttcattttt tgcagcaata
     3301 aataaaatag aaaaaatgca ctcaacgtcg atattcattc aagaatggct gtaaacggcc
     3361 gattccaggt ttattcgttg agagattaag tagtaagcta ttactgaagg gggagttctc
     3421 tgtgataagt cgaaaactcg actctgagaa cattggaaaa tcaaatcgca ggcttgttct
     3481 tcaactactg aggaaaaacc tgatcacgac gaggagagat cttgcctccg tctccgggct
     3541 aaatcccagt actgtgacta aaataatccg tgagttttta cttgccggtc tttgcgagga
     3601 aatcagcacg gaggtgaccg acaggatcgg tagaaaggcc atagaactcc gtctgaatcg
     3661 taaggcctat gtatcgatcg tcgtagatat tggggtcgaa gaaaccagca taggcaaggg
     3721 gtatttcgat ggaacgacct caacaataag tagtttcagt actccttcaa cattcgagga
     3781 attcacagag gttctcgttg agaagacggg agaggtagtg aaaaaaatac ctaaatacaa
     3841 atttcttggg tactccatat ccgtccccgg catggtagat gttgaacgat ccaagatagt
     3901 ttacgtgccg cacctcggat ggaaagatgt tttcctgaag gactctctct caaaaagata
     3961 tcctgtcgta ctggacaacg aagccaatct ctcactgata gcggaaaagt ggaacaatcc
     4021 cggtcttgcc ggactcggag atatcgtatt cgtatatgtt tcagaaggta tcggttgcgg
     4081 gatcatgctg ggcggacaga tccaccgggg ccgcgattac tcagccggag aaatcggcca
     4141 tatgacagtg cagctcgatg gacctaaatg ctactgcggc aacagtggtt gctgggaaac
     4201 cttcgcatcc agcgaggcaa ttgtgagaaa ggcgcaatcg gagggacttg agctgaaagg
     4261 ttcaaataat aacgaaagat acctggatat tttgaagaat tatgaaaaga gtgaatattc
     4321 ctttttgatt gaggaaatag aaaagagttt ggccgttgga atagtcaaca tagtgaattc
     4381 actggacccg gaggtaatcg tccttggggg agtggcttcc atgcttccgg aagaagcgct
     4441 caaacgcctc accggaatcg tcaactccag agttctgtat gcaaccggac agaacataga
     4501 agtgaggaga agcattctcg acactcacgg caagaccagt tcgaatatga taggcgcagc
     4561 actacatatc atcgagcgca gagtagatga tttcatctga aggggcgatc tatggaggtc
     4621 tttgttggaa tcgacatcgg tacaacctca gtgaaggcga tagccgttga tagcaagggc
     4681 aagatacaaa aatctctctc tagaagcctt cctttgcata cgccaaaacc gggatgggtc
     4741 cagcaaaacc cgaaagattg gtggcaagct gtcgttgaag tgctctcaga gctttctaaa
     4801 agcggttgta gaatagccgc catctccgtg agtggtcaga tgcacagcct ggtcgcacta
     4861 gacgatagag atgaaccggt gtatccatcc attctctggt gcgatcaacg aaccgagaag
     4921 cagtgcgaag aactcaccgg taaactcggt ggtgagaggg cagtcattga atccttcggg
     4981 aatcccattc ttacaggctt cacccttcca aaatttttat ggctctcaga aaacgaacct
     5041 gaacgttttt cgacgatcat caaatggatg cttcctaaag attacgtagc ctaccgcctc
     5101 acgggaagat ctacgatcga ctactcggac gcttccggca cctcggcctt gactctggaa
     5161 ggagagtttt cgacacgggc gcttgaactg tgtagcataa atcgatcatc aaatcccgaa
     5221 ctcgtcagtt cgggcgaagt cataggatct gtactcgaag gaacaatccc gggactggag
     5281 ggaatcccgg tggtggctgg aggagccgac aacgcctccg cggccttcgg ttgtggagtg
     5341 gaatggcctg gcgataccat ggtcagcctc ggaacttcgg gc
//
LOCUS       DAOOLY010000168         5341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18499_length_5341_cov_5.039917, whole genome shotgun sequence.
ACCESSION   DAOOLY010000168 DAOOLY010000000
VERSION     DAOOLY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18499_length_5341_cov_5.039917"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLD15_08550"
     CDS             <1..567
                     /locus_tag="PLD15_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB63997.1"
                     /translation="WILEKVGLRPEYMYRYPHEFSGGQRQRIGIARAIILNPELVICD
                     EPVSALDVSVQAQVINLLEDLKHDFQLTYLFIAHDLAVVKHICDRISVMYLGKIVETA
                     ETDELFRNPLHPYTQGLLASIPIPNPHMRKIGKRELVKGDIPSPVDPPDNCRFVKRCP
                     KAFDRCLKEAPRLTETGSGHYVSCFLYE"
     gene            complement(585..1871)
                     /locus_tag="PLD15_08555"
     CDS             complement(585..1871)
                     /locus_tag="PLD15_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPB63998.1"
                     /translation="MKRIVPTILILCVSATFLYARSEALAERYLQLFLRGSFYLANEM
                     QSVKVRKDYTVDDMQKELERLRENYGNYLFIFSTETSEIRGYTVYIFHSQFEKGYIDF
                     NIVIDNFGKVDAFVVQPTLQPGAIPDYIDTSRFDEFEITIGSDRWKLPGVLTVPKNLD
                     RYPLVILIHDSGALDRDSTIGPNKPFRNIAWGLATRGVAVMRYDKRTFVFGEKLSQAS
                     PSIETEIIEDVMNAVNMASKMPAVSSIFLVGHGLGGKIAPTVAARNENVDGIVLMGTS
                     ARRELQVIIDRQNYVSELFYSDRERQQLKLLIDYLQKALDGKLLPGTPVLAATAGYYY
                     EIDALDPIETIKKLNIPVLILQGGADYESTTDDYMTFMNSLWTSLNVYFQLFPNLDHY
                     FMPVEREKSTPDDYYEFRHVDRELVESLFSWIFVFN"
     gene            2400..3509
                     /locus_tag="PLD15_08560"
     CDS             2400..3509
                     /locus_tag="PLD15_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB63999.1"
                     /translation="MPRGGGSGSRSSGGSSSSNRSSGGRSGGSFSSGRSSSGSSSSSS
                     SSRKSSGGFFSGGTGSSSSSSSNKVSNRKIGVPIIGGLGTFRRVSNSGGGVNQSVGGV
                     GSGCSGCSGCSFVALGIIVVAVIIVIVIVSAQGAPNIQTVSKNEITRSTIVRKPLPDG
                     SVKETAYFTDELGWITNQNQLLEGMKHFYKKTGVQPYLYITDTIYGSHFPSEEQLNAF
                     THELYERLFTDEAHLLLVFFEYEERYMDRYVAGVQAKTVIDEEAADILLDYIDRYYYD
                     TGLTDDAFFSKAFTDAANRIMTVTKSPWISVLIVFGIVVAILILFAWWSRVRRQKYLE
                     AKQTEEMLKVPLEKFGDKEAEDLSKKYEENNSNEK"
     gene            3577..4254
                     /locus_tag="PLD15_08565"
     CDS             3577..4254
                     /locus_tag="PLD15_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HPB64000.1"
                     /translation="MGILERFNDIIKSNINALLDKFEDPSKMIDQYMREMIEDLAEVK
                     KGTASVMAEETRTKRLVDENAAEVAKYANYAKRALMSGSEEDARVFIAKKQELEDAGA
                     GLMTAYAAAHENAIKMRQMHDKLVENIETLKARREMIKAKVSVAKTQKTINEAASTSG
                     RTQGAMDAFNRMEDKAQKMLDEATAMAELNSEPIDKAKALEEKYASKSSFSVEEELNR
                     MKSELEK"
     gene            4433..>5341
                     /locus_tag="PLD15_08570"
     CDS             4433..>5341
                     /locus_tag="PLD15_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPB64001.1"
                     /translation="MKSKAIPAFFLLLFLSFTCIFATRIMVIHSYDEDYVWSREMLEG
                     IKSVFADESIEWKVFYMDTRLQTNEEWKIKAGKMAAREVEAFQPDIVIACDDNAQEYF
                     AKDFTALPLIYCGVNLDPARYSYPRSNATGIRETLFSKETVEMLLDFFPQVREVHFIG
                     DDSETVAGMREQLEATSFGDLSTKIHTVGSFNEWKEVVKSLSGEGNAIVILADRTLKD
                     EHGRTVSTEYVTEWTVKNTGLAVFSVLDFVVADGAMAGVVNSAFEEGKRAGLIALKIL
                     SGLSPDEIPPENSSNGRSMVNIETLLS"
BASE COUNT         1506 a         1235 c         1347 g         1253 t
ORIGIN      
        1 tggatactcg agaaggttgg cctgagaccg gaatatatgt accgctatcc gcacgagttt
       61 tccggggggc agagacagag gataggtatc gcccgagcga taattctgaa tcccgaactc
      121 gtgatctgcg acgaaccggt atcggcattg gatgtctccg ttcaggccca ggtaataaac
      181 cttctggagg accttaagca cgacttccag ctcacatacc ttttcatcgc ccacgatctg
      241 gctgtggtaa aacacatctg cgacaggata tcggttatgt acctgggtaa gatagtggag
      301 accgccgaaa cggacgagct cttcagaaac cctttgcatc cttacacaca gggacttttg
      361 gcctcgatac cgatccccaa tccccacatg agaaagatcg gcaagagaga actcgtcaag
      421 ggagatattc cctcgccggt cgatccaccg gataattgcc gattcgttaa aagatgccca
      481 aaagccttcg atagatgctt gaaagaagcg ccccggctaa ccgaaacggg aagcgggcac
      541 tacgtctcct gttttctgta cgaatgaagc aatgccgaga gggatcagtt gaagacgaag
      601 atccaggaga agagactctc gacgagctca cgatcgacgt gacggaattc gtagtagtcg
      661 tcgggagtcg acttctctct ctctaccggc atgaagtaat ggtccagatt gggaaagagc
      721 tggaagtaaa cgtttagact ggtccagaga gagttcatga aagtcatata atcatcggtc
      781 gtcgattcat aatctgcccc accctgaaga atcaaaaccg gtatattcaa tttctttatc
      841 gtttctatcg gatcaagggc atctatttcg tagtaataac ctgccgtcgc tgccagaaca
      901 ggcgttcctg gaagcaactt tccatccagg gctttctgga ggtaatctat cagaagtttc
      961 agctgctgcc gttccctgtc cgaatagaag agctccgaga cataattctg gcggtctatt
     1021 atcacctgca attcccttct ggccgatgtt cccataagta caataccgtc tacattctca
     1081 tttctagctg ccacggttgg ggctatcttg ccacccaatc cgtgaccaac aagaaagata
     1141 gaagagacgg ccggcatctt cgaggccata ttcacagcat tcatcacgtc ctcaataatt
     1201 tctgtctcga tactcggcga agcttgagac agtttttctc cgaagacgaa agtccttttg
     1261 tcgtaacgca ttacagccac accgcgcgta gcaagtcccc aggctatgtt tctgaaaggt
     1321 ttgtttggtc cgatcgtcga atctctgtcg agggcgcccg aatcgtgtat gagaatgacc
     1381 aggggatatc tgtccaggtt cttcggcacg gtcaacactc ccgggagttt ccatctgtcg
     1441 ctcccgatgg ttatctcaaa ctcgtcgaac cggctcgtat ctatgtagtc ggggatggcg
     1501 cctggttgaa gagttggttg cacgacaaaa gcatcgacct tcccgaaatt atcgatcact
     1561 atgttgaaat ctatgtagcc cttctcgaat tgcgaatgaa agatataaac agtgtatccc
     1621 cggatctcgc ttgtttcggt agagaagatg aagagataat tgccataatt ttccctgagg
     1681 cgctcgagct ccttctgcat gtcgtccacg gtgtagtctt tccgcacttt cacgctctgc
     1741 atctcgtttg ccagataaaa gctcccccgc aggaacagtt gaagatacct ctcggccaga
     1801 gcctcagacc tggcgtatag aaaagttgcc gaaacgcata aaatcaaaat ggtcggtacg
     1861 atccttttca agacaacgct tctcccaaaa tagactgaga ggcaaataca cacctcgatt
     1921 aaatattaaa ccacactgca ccgtctttac tattaagaga tagaggcaag attaaccttc
     1981 acaaattcgg tccaggcttg caagaaggaa gcatctttga aatgttccta attaagagat
     2041 gaaaggcatc tcactcttct caaaggacac cgggtaagag atggctctat tatgcttgga
     2101 acctgcaact tgaaaccact ctcgatggac ggactcataa tccgtgactc gaaagttgat
     2161 tgaatagctt gtataattaa actgagtcaa taagttggaa taaagataac aacgatcggt
     2221 ttgtggcttt gatatctttg aatacaccca tcaagacggg ggaatataaa agcttattgt
     2281 accggaaccg gcgccgggat caaagtcaac ggtctattgt gtattgagcg gttggattta
     2341 tatgttttag tgattataat taattgaaag tatgcctgta tttgaaggga gatagagaaa
     2401 tgcctagagg tggaggttcg ggtagcagga gcagtggtgg ctcttcttcg agcaacagga
     2461 gcagcggcgg gcgtagcggc ggttcttttt ccagcggtag atctagcagt ggaagtagtt
     2521 cctcttccag cagtagcagg aaaagcagcg gcggcttctt cagcggcgga accgggagca
     2581 gctcgagcag ttcaagcaac aaagtcagca acagaaaaat cggcgttccc attatcggag
     2641 gactcggtac tttcagacgt gtcagtaaca gcggtggagg agtcaatcag agcgtcggag
     2701 gagtcgggag tggctgtagc ggttgcagtg gctgcagttt tgtggcgcta ggaataatcg
     2761 ttgtagcggt gattatagta atagttatcg tttctgccca gggagcgccc aacatacaga
     2821 ctgtctccaa gaacgagatc accagatcga cgatcgtgag aaagccctta cccgatggct
     2881 ctgtgaagga aaccgcttat ttcacagacg agttaggctg gatcaccaat caaaaccagc
     2941 ttcttgaagg aatgaagcac ttctacaaaa agactggcgt acagccttat ctttatataa
     3001 ccgatacgat atacggttcg cactttccgt ccgaggagca attgaatgca ttcactcatg
     3061 aactttacga aaggcttttc acagatgaag cccacttatt gctcgtattc ttcgaatacg
     3121 aggaaagata catggacaga tacgttgccg gagtacaggc caaaaccgta atcgatgaag
     3181 aggcggccga tattctgctc gattatatcg atcgctacta ttacgatacc ggcctgacag
     3241 atgacgcttt cttcagtaaa gccttcacgg atgcggccaa caggattatg acggttacca
     3301 aatcaccctg gataagcgta ctgatagttt tcggaatcgt agtcgctatt cttatactat
     3361 tcgcctggtg gagccgggtg agaagacaga aatatctcga ggccaaacag accgaagaaa
     3421 tgctcaaggt accgctcgaa aagttcggtg acaaagaggc cgaagatctt tcaaaaaaat
     3481 acgaggaaaa caatagcaac gaaaagtaac gtttaggcca aaatgagcag atttgtctat
     3541 gacggtcagg cttaaactaa agggaggaca aagaggatgg gtattttgga aagattcaac
     3601 gacattatca agtcgaacat caatgcatta ctggacaagt ttgaagatcc atccaagatg
     3661 atcgaccagt acatgcggga gatgattgaa gatctggccg aagtaaagaa aggcacggcc
     3721 tccgttatgg ccgaagaaac ccgcaccaag cgtctcgttg acgaaaacgc ggccgaagtg
     3781 gcgaagtacg ccaactacgc caaaagagct ctgatgtccg ggagcgaaga agatgcccgt
     3841 gttttcatcg ccaaaaagca ggagcttgaa gatgccggtg ccggccttat gacggcctac
     3901 gcggcggccc acgaaaacgc tataaagatg cgtcagatgc acgataagct ggtagaaaac
     3961 atcgaaactc tgaaggcgcg gcgcgagatg ataaaagcca aagtctctgt ggccaagact
     4021 cagaagacga tcaacgaagc ggcctcaact tctggaagaa cacagggagc gatggatgcc
     4081 ttcaacagaa tggaggacaa ggcccagaag atgctggacg aggcaaccgc aatggccgaa
     4141 ttgaacagcg agcccatcga caaggcaaaa gccctggaag agaaatatgc ctcaaagagc
     4201 tctttctcag ttgaagaaga actgaataga atgaagagtg aactcgaaaa gtagcagcaa
     4261 ccgatattaa ccctaccagg accccgctca ctgaaaaatg tgcggggtct ttttcattct
     4321 gtgagatgcg agatactttc atttcaggtg tcgcttacgt ctccggattc gatctatcct
     4381 gagctataat tcaaacgttg agatgtttct aaagggaaaa gggggagaaa tcatgaagag
     4441 taaagcgata ccggcgttct ttctgttgct ctttctttct tttacgtgta ttttcgccac
     4501 caggatcatg gtgattcata gctacgacga agattacgtg tggtcgaggg agatgcttga
     4561 aggtatcaag agcgttttcg ccgacgaatc gatcgaatgg aaggtcttct acatggacac
     4621 acgtcttcaa acaaacgaag agtggaagat aaaagccgga aagatggccg ccagggaggt
     4681 cgaagctttt caacccgata tagtcatcgc ctgcgacgat aatgcgcagg agtatttcgc
     4741 gaaagacttc accgcgctcc cgctaattta ctgcggtgtc aacctcgatc cggcaagata
     4801 ttcgtacccg cgcagcaacg ccacgggtat cagggaaacc ctattttcga aagagacggt
     4861 cgagatgctt ctcgatttct ttccacaggt tagagaggtc catttcatcg gtgacgatag
     4921 cgaaaccgtc gccggtatga gagaacagct cgaggcgact tcctttggcg atttatctac
     4981 gaaaatacat acggtcggca gtttcaacga atggaaggaa gttgtgaaaa gcttatccgg
     5041 agaagggaac gccatagtta tcctcgccga caggacactc aaagacgaac acggaagaac
     5101 ggtaagcact gaatatgtta ccgaatggac ggttaaaaat acgggtttgg cagtcttttc
     5161 tgttctcgat tttgtagtgg ccgacggcgc gatggccggt gttgtgaact ccgctttcga
     5221 ggaaggtaaa cgggccgggt taatcgcctt gaaaatactc tccggacttt ctcccgatga
     5281 gatcccaccc gagaattcct caaacggccg gtcgatggtc aacatcgaga ctcttctctc
     5341 g
//
LOCUS       DAOOLY010000169         5339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18510_length_5339_cov_4.236942, whole genome shotgun sequence.
ACCESSION   DAOOLY010000169 DAOOLY010000000
VERSION     DAOOLY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18510_length_5339_cov_4.236942"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLD15_08575"
     CDS             <1..868
                     /locus_tag="PLD15_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64002.1"
                     /translation="VKADPEHFKYWLPEGNLVFLNLNNLKWPFDSYNLRAAIASAIDP
                     FEITRIMASGAVPASPAGVKASYLRLAENGLSEYGIEFDPDYARYLIEREGYKLSRSG
                     IYEKDGKALSLNLYVPTGWTDWIMGAEEVARQLKEVGIEVIVTLAAWPSPYQDMIFNG
                     NYEMLFGISVTGTSPYYQFDNWVHSKHWAPIGERTKNYYGMRYKNDRIDELLDSYRKL
                     TDPQAQDEVMEEVIAIFLRDLPSVPMFFNPVWFEYSTRNFVGWPSAENPYAEPRPTGM
                     DKMPVLLSVHLR"
     gene            1063..1959
                     /locus_tag="PLD15_08580"
     CDS             1063..1959
                     /locus_tag="PLD15_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64003.1"
                     /translation="MPGDPAQDLIDKMQGIPLESLEAIRAAFGVDSNDPLLVQYGKYL
                     LNLLKGDLGISISRFPTPVWRVLQVAIPWTVGLMGTATIISFFLGTLIGIGVAWKRNT
                     KLASFTVGLFIFVRSFPYFWLGLLLIYFLAFRLPIFPLGNAYTPQLQRGTMEFFFSVL
                     RHAILPALTIVISSMGAWILTMRNNMINVLAEDYITVAEAKGLPLRRIKTLYAAKNAI
                     LPSITGFAMSLGFVVGGGLLTEMVFAYPGVGLMLYQAVQSKDYPLMQAIFLFISAAVL
                     VANFIADIAILILDPRVRDGGK"
     gene            1959..2936
                     /locus_tag="PLD15_08585"
     CDS             1959..2936
                     /locus_tag="PLD15_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64004.1"
                     /translation="MLKDTFSLFIENRKALFGLMILVFFSGVAIFAPLIAPYDPKTNK
                     VDVLRIVEEEMGRTTTVEREQIDEFTERRVYTQSIATTYERVTTKLPLNARPSRQHLL
                     GTTKAGQDLFSQMVYGTRITLAVGLVTGLFTTILALTLALLAGYFGGVVDDIISLFTN
                     VFLVIPSLPLMIVIAAYITVRGVIPIVLILGLTSWPWSTRILRSQVVSLRNRDFVRVS
                     RILGERPLYILFREILPNMISLVMASFFTSTMAAIMGEATLEFLGLGNVSIVSWGTIL
                     YWAQNSGALLSGAWWWFLPPGICIALIGTSFALMNFAIDEISNPKLRKR"
     gene            2933..3928
                     /locus_tag="PLD15_08590"
     CDS             2933..3928
                     /locus_tag="PLD15_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64005.1"
                     /translation="MIELDRLEMTPLLQCKNLVAGYRIKSGFVHAVDDVSFDLDRGGF
                     LGIAGESGCGKSTLAYAIMRLLKDNATIESGSLLFKGADLAKLDEEQMKKIRWVDISM
                     AFQSAMNALNPVLSVGEQLTDAIHAHEEATQSDARKRALEVLKLVDIPKDRFNSYPHQ
                     LSGGMKQRVMIAMALILNPELIIMDEPTTALDVVVQRTIIEKIQELRKTLGFSVIFIT
                     HDLSLLVEISDTLAIMYAGKIVEYGASESLFNRPLHPYTKGLMNSFPSLTGMIKRMGG
                     IEGKPPDLLKPPEGCRFHPRCPQAIPICRESCPSLEEIADRHSVACFLHKGAEIL"
     gene            3925..4893
                     /locus_tag="PLD15_08595"
     CDS             3925..4893
                     /locus_tag="PLD15_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64006.1"
                     /translation="MNENILVLEGVNKHFPVNSFRMHGKVVHAMDDVSFSLRRGEVLA
                     IVGESGSGKTTTANVMARIYKQTSGRVTFEGREISKNMSREDELRYHKKVQMIFQDPF
                     GALNPTRTVGRIMERPFVIHKIAGRRKAVEESIDKILIQVGLEPPEQFTRKFPHELSG
                     GQRQRVNIARAFAVEPELILADEPTSMLDVSNRMSIMNMMLYLKESHSVSFIYITHDL
                     SGARYMSDRIIVMYAGMIMEIGPAEKIINNSFHPYTRLLKSAAPSPEMGLKRKRLITG
                     GDIPSLIDPPGGCRFHPRCPFVSDICSLEVPAMKKIDEGHYVRCIL"
     gene            complement(5005..>5339)
                     /locus_tag="PLD15_08600"
     CDS             complement(5005..>5339)
                     /locus_tag="PLD15_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB64007.1"
                     /translation="EDFMQTKRTKRLGRIKPTLPESDFILGDINLVLPRRISVSITEF
                     IEKLSEVIPGVAYPDNLMYAVEVKFYSNKIDNDRYRNLKFIGDCSGHTRSITYATGHG
                     RLLGSSLK"
BASE COUNT         1337 a         1247 c         1356 g         1399 t
ORIGIN      
        1 cgtcaaggcc gaccccgaac acttcaaata ctggcttcct gaaggaaacc tagtcttctt
       61 gaacctcaac aatctcaagt ggcccttcga cagctacaac ttgagggcag ctatagcctc
      121 ggcgatagac ccgttcgaga taaccaggat aatggccagt ggagcagtcc ccgcctctcc
      181 agccggcgta aaggcgagtt accttagact ggccgagaac ggtctcagtg aatatggtat
      241 agaattcgat cccgattacg caaggtatct aattgaaagg gaaggttaca agcttagcag
      301 gagcgggatc tatgaaaagg acggtaaggc tctctctctg aatctctatg tgccgacggg
      361 gtggaccgac tggataatgg gagctgaaga agttgcaagg caactgaaag aggtgggaat
      421 cgaggttatc gttactctcg ccgcctggcc ttccccttac caggacatga tcttcaacgg
      481 aaactacgag atgctttttg ggatctccgt cactggaaca agcccctatt accagttcga
      541 caactgggtg cattccaagc actgggcacc tataggagag aggaccaaga attactatgg
      601 aatgcgctac aagaacgaca ggatagatga actcctcgac agctacagga aactgacgga
      661 tcctcaggcc caggatgagg taatggaaga ggttatagcc atcttcctga gagatcttcc
      721 ttccgtaccc atgttcttca atccggtatg gttcgagtac agcaccagga atttcgttgg
      781 ctggccgagc gcagaaaatc catacgcaga gccgagacct accgggatgg ataagatgcc
      841 ggtattgctt tctgtacacc ttaggtagtt tttcgcgtgg cctcgcgggc aactgagccc
      901 gcgaggttca tcctcattca cgagaggggg gtaaggaagg cctgctttcc gtataaggaa
      961 gacgctttct gaacagcatt ggcctatttc gcaagaaaaa ttggattgtt gttattcgcc
     1021 ctttttgttg cagttactct gaatttcttc attccgagaa tgatgcccgg tgatcctgca
     1081 caggatttga ttgacaagat gcagggtatt ccgttggaat cgctcgaagc aataagggct
     1141 gcctttggcg tcgattctaa cgatccgttg cttgtccagt acggaaagta tcttctgaac
     1201 ctcttgaagg gcgatctggg aatttctatc tccagattcc ctactccagt ctggagagtc
     1261 ttgcaggtcg ccattccctg gactgtcgga cttatgggta ctgcaacgat catcagtttc
     1321 tttctgggca cattgattgg aataggcgtt gcgtggaaac gtaacacgaa gctcgcttcc
     1381 ttcactgtcg ggctcttcat tttcgtgcgt tccttcccgt atttctggct cgggctgctg
     1441 ctgatatact ttctggcttt caggcttccg atatttcccc tcggaaatgc ctacactccc
     1501 cagcttcaga gagggacgat ggaattcttt ttctctgttc taagacacgc gatacttcct
     1561 gccttgacaa tcgttatctc ttctatgggg gcgtggatat tgaccatgag gaacaacatg
     1621 atcaacgtgc tggccgagga ctacatcacc gttgccgaag ccaagggcct cccgctgaga
     1681 agaatcaaga ctctatacgc cgcaaaaaac gccattctgc catctataac tgggtttgcg
     1741 atgtctctgg gatttgtcgt tggaggcggt ttgttgactg aaatggtctt cgcctacccg
     1801 ggagtaggct tgatgctcta tcaggcggtt cagagcaagg attatccgct gatgcaggcg
     1861 atatttcttt tcatttcagc ggccgttctc gtagcgaatt tcatcgcgga tatcgcgatt
     1921 ctgattctcg atccccgtgt tcgggacggt ggcaagtgat gttaaaggac actttttctc
     1981 tcttcataga gaaccgaaag gctttgttcg ggctgatgat acttgtcttt ttctccggag
     2041 ttgcgatctt tgccccattg atcgctcctt atgatccaaa gacgaacaag gtcgatgtcc
     2101 tgaggatagt ggaagaggag atgggtagaa ccacaactgt tgaaagggag cagatcgatg
     2161 aattcacaga acgaagagtc tacactcagt cgatcgcgac tacatatgag agagtaacaa
     2221 caaaactccc gctaaatgca aggccttcca ggcaacactt gctcggtacg acgaaggccg
     2281 gacaggatct tttctctcag atggtctacg gaacgagaat cacgcttgcc gtgggtcttg
     2341 taaccggact gtttactacg attcttgctc taacacttgc gcttttggcc gggtatttcg
     2401 gaggcgttgt tgacgacatc atctctctgt ttacgaacgt cttccttgtt atcccgagcc
     2461 tcccgttgat gatagtcatt gcagcgtata taacggtcag gggagttatt ccaatcgtcc
     2521 ttatactagg acttaccagc tggccatggt ctacgagaat actgcgttct caggttgtga
     2581 gtcttagaaa cagagatttc gtaagggtgt ccagaattct cggcgagagg ccgctataca
     2641 tactcttcag ggagatactg ccgaatatga tctcgctggt aatggcatcc ttctttacat
     2701 caacgatggc ggcgatcatg ggagaggcta cccttgagtt tttgggactg ggcaatgtct
     2761 cgatagtgag ttggggaaca atcttatact gggcacagaa cagcggagct cttctgtcgg
     2821 gtgcatggtg gtggttcctt cctccgggta tctgcatagc cttgatcggg acttcgtttg
     2881 cgctcatgaa ctttgccatc gacgagatct caaatcccaa gcttagaaag agatgattga
     2941 gttggacaga ctcgagatga ctccactact tcaatgcaaa aaccttgtag caggttacag
     3001 gatcaagagc ggcttcgttc acgcagtgga cgacgtctcg tttgacctgg atagaggggg
     3061 ctttttgggc atcgcaggcg aatcgggatg cggcaaatca acgctggcat acgcaattat
     3121 gaggcttctc aaagacaacg ccacaatcga aagcggaagc cttttattca aaggcgccga
     3181 tcttgcgaaa cttgacgaag aacagatgaa aaagatacgg tgggtggata tttcgatggc
     3241 ttttcaatca gccatgaatg cactaaaccc tgttctttcg gttggtgagc agctcactga
     3301 tgccatacat gctcacgaag aggcaacaca atcagacgct cgaaagcggg ctcttgaagt
     3361 gctgaagctg gtagatattc ccaaagacag attcaactct taccctcatc agctttcggg
     3421 aggtatgaag cagagggtta tgatagcgat ggccttgatt ctgaatcccg agctaatcat
     3481 aatggatgag ccgacaacgg cactggatgt tgtagtacag cgaacaataa tcgagaagat
     3541 tcaggaactg aggaagactc taggcttctc agttatattc atcactcacg atctctcttt
     3601 gctagtggag atcagtgata ctctggccat catgtatgct ggcaagatcg tggagtatgg
     3661 agcctccgaa tctcttttca atcggccctt acacccatac actaaggggc ttatgaactc
     3721 cttcccatcc cttacgggaa tgataaagcg gatgggcgga atcgagggaa agcctcccga
     3781 ccttttgaaa ccacctgaag gctgtagatt tcaccctcgt tgtccgcagg caatacctat
     3841 ttgcagagag tcctgcccct cactggagga gatcgccgat cgccacagcg tcgcttgttt
     3901 tcttcacaaa ggagctgaga tcctgtgaac gaaaatatcc tggttcttga aggagtcaac
     3961 aaacacttcc cggtaaactc tttcagaatg catggaaagg ttgttcatgc catggatgac
     4021 gtctcttttt ctctgcgaag aggagaagtg ctcgcgatag ttggcgaatc cggcagcgga
     4081 aaaaccacta cggcaaacgt gatggccaga atttacaaac aaacttccgg tagagtcacc
     4141 tttgagggca gagaaataag taaaaatatg tcgcgcgaag atgaactgag atatcacaaa
     4201 aaagtccaga tgatctttca ggatcccttt ggagccctaa accctacacg aactgtcgga
     4261 aggataatgg agcggccttt cgtgattcac aaaatcgccg ggagaagaaa agccgtagaa
     4321 gagagtattg ataagatact catccaggtg gggctggaac cgcctgagca attcaccaga
     4381 aagtttcccc acgaactatc tggaggacag aggcaaaggg taaatatagc gagagctttc
     4441 gctgtcgagc cagagctgat tctggccgac gaacccactt cgatgctcga cgtttcgaac
     4501 agaatgagca ttatgaatat gatgctttac cttaaagaaa gtcacagcgt atccttcata
     4561 tacataaccc acgatctttc cggtgcgcgc tacatgagcg acagaataat tgtcatgtat
     4621 gcgggcatga tcatggagat cggcccggca gaaaagatta taaacaactc gttccatcca
     4681 tacacaaggc tgctcaaatc ggccgctcct tccccggaga tgggtttgaa gcggaagcga
     4741 ctgattacgg gtggagatat tccgtcgctg atcgatcctc ctggtggttg tcgttttcat
     4801 ccgaggtgtc ctttcgtaag cgatatttgt tctctggagg tgccggcaat gaagaagatc
     4861 gatgaaggtc attatgtgag gtgtattcta tgacaaatct gctgaggttc caacttagta
     4921 ttgtgtttat cgtaaagaag gggagtggtc gattttgaag ctattatttt ttttatcaac
     4981 cgcttccccc agttaggtga agccttattt aagcgaactt cctagcaatc ttccgtgtcc
     5041 ggtggcgtac gttatcgatc tggtgtgtcc gctgcaatcg ccgatgaatt tgaggttcct
     5101 gtagcgatcg ttgtctatct tgttggaata gaacttcact tctaccgcgt acatcaggtt
     5161 gtccggatat gccactcctg ggataacttc actcagcttt tctatgaact ccgttatgga
     5221 gacagaaatt ctcctcggaa gtacaaggtt tatatctccg agtatgaaat cgctttccgg
     5281 gagggtcggt ttgattcttc ctagcctttt ggtccgcttg gtttgcatga aatcttcgt
//
LOCUS       DAOOLY010000170         5337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18520_length_5337_cov_4.957781, whole genome shotgun sequence.
ACCESSION   DAOOLY010000170 DAOOLY010000000
VERSION     DAOOLY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18520_length_5337_cov_4.957781"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLD15_08605"
     CDS             <1..803
                     /locus_tag="PLD15_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB64008.1"
                     /translation="YWFEKADIDGWRLDTVEYLHPDFVRAVRETSKEMKPEAYVLGEL
                     LHLGTSWFKGGYLDGAMNYRLRKYLLSFFVFNNIDAKTFGEKLYVLRRSYPDWANYAM
                     YNMIDSHDRPRITTLCNGRKEIVRLILLFLFAYPGVPAIYYGDEIGIEGGNDPDCRRS
                     MIWDERLWDRELLEFCKRLIELRKTSPALRKGAFIPGIAENGLFTFEREYGNERVVVC
                     LNNSPVAGEYEIGKPYDVLLSGSARIDSFTLSLDPYGFAVLRKTAENV"
     gene            complement(861..3599)
                     /locus_tag="PLD15_08610"
     CDS             complement(861..3599)
                     /locus_tag="PLD15_08610"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB64009.1"
                     /translation="MKKWMIYTLIAAVAVAIVLIATIPALLNKEPVMELPVQVVNQGE
                     KLTVDLRAFIKDEKADEVTLEKVDGPGTITGSVFTFEPAFKYVGEVIAKIKATDKQGK
                     NSTGELKINVIRVNRPPEIDTTPLKVLEGESMSLDLLTIVKDPDNDELSLAVDGPGKL
                     EGNTYVYAPGYLDAGKKVLRITAKDSEGNETVRDVQLEVVDVNAPPALVVSDQTVREG
                     DSLAVDLAALVSDAEGDGVTLTLIEGPGEIVDGVFIYKPGFAEAGESVVSISAKDSRG
                     GESTSTFKVTVTETNRPPRIFLSDMVISEGEELVVDLSSRLIDPDNDPLEITVEGPGA
                     VQENRYVYTPGYRDAGDKTVTIMVSDGKGGTGRASFTILVQDANRPPEGSIANETIFE
                     GERLNLYLKAFAYDPDGDGIFFELLEGPGSVEGDSYTYEPDFESAGLKEVKIKVSDSN
                     GEEKILVFEIDVVDVNRAPVAVLPSIAASIKETFTLGLDLKSFFTDPDGEELSFAILE
                     GPGSIEGGNYKYTPSYNDQGEKTVTIVARDSSGLSANLPIIISVMDLNRSPRILLSDK
                     MIDEGANLVVDLRMQSIDLDGDELTFTLMEGPGSVENAIYTFEPSFDESGDHTVTIQV
                     SDGLASVTGEFMVEVVDVNRTPETTIKEIKSSIREGFSLGMNLNTFFSDPDGDELTLS
                     LEGPGTLEGNGYTYSPGYEDSGEKFILVTASDGRGGGVTLPIIITVLDVNRLPEIKEP
                     FPEVTLEEGKTVEIELGDFISDPDGDDLLFEIREGPGSIVDGKYVFEPRTGEAGQKDV
                     MIKVSDGKSELTVPLKIFVEAGHRVVSITYALFSSGIDKVKLVAGSHEIISSGQRAEL
                     EVDWPLDFDTVSIWKVTEESAEFIGAARITASSSSRNIPVVSEGGETKGSVRIELK"
     gene            complement(3691..4227)
                     /locus_tag="PLD15_08615"
     CDS             complement(3691..4227)
                     /locus_tag="PLD15_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HPB64010.1"
                     /translation="MIHRIERIAELSTARMTTLLIFEPEKESIVPGTSQRYIFIVPFQ
                     VRGYLDLKSIDSNSIEIYEGESRRIKLVLPPPELEVTIPQSKIPDIRVINQSGVLVKV
                     FGNRDMLKTFQSYSKEIEQQALISAHEMGIEETSRESARNFFHGLLLGMGFDEVVVLF
                     KELERPQSLRESTNTAIR"
     gene            complement(4345..4638)
                     /locus_tag="PLD15_08620"
     CDS             complement(4345..4638)
                     /locus_tag="PLD15_08620"
                     /inference="COORDINATES: protein motif:HMM:NF036614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HPB64011.1"
                     /translation="MGSIAGMIANPFSIFGLISAIVALVMGLRLRRTKRELETFIHTK
                     APVNLEIGLDSMMKYFRIQGVLIILAIVFVLITLIAVIVMGAAFNFNFDFYNT"
     gene            4968..>5337
                     /locus_tag="PLD15_08625"
     CDS             4968..>5337
                     /locus_tag="PLD15_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPB64012.1"
                     /translation="MEYRKVGRSGLRISELSLGSWLTFGAQLDIENAREAMRRAYREG
                     INFFDNAEAYASGMSESMMGEILKEFKREEVVVSTKIFWGGNKPNQKGLSRKHLLEGT
                     WNSLKRLQLSYVDILYAHRPD"
BASE COUNT         1247 a         1400 c         1279 g         1411 t
ORIGIN      
        1 gatactggtt cgagaaggcc gatatcgacg gctggcggct cgacactgtg gaatacctgc
       61 atcccgattt cgttcgcgcc gttcgagaga cctccaaaga gatgaagccg gaggcttacg
      121 ttctgggcga gcttcttcat cttggaactt cctggttcaa gggaggttat ctggacggag
      181 cgatgaacta cagattgaga aagtatcttc tctccttttt cgtcttcaac aacatcgacg
      241 cgaagacctt tggagaaaag ctctatgtct tgcgacgtag ctatcccgac tgggcgaatt
      301 atgcgatgta caacatgatt gacagccacg atcgccccag aatcaccaca ctctgcaatg
      361 gcaggaagga gatagtcaga ctcatactat tgttcttatt cgcctacccg ggcgttcccg
      421 cgatctatta cggcgacgag ataggcatcg aaggcggtaa tgatccggac tgccggaggt
      481 cgatgatatg ggacgagaga ctctgggacc gggaacttct tgagttctgc aagaggctca
      541 tagagcttcg aaaaaccagt ccggctctga gaaagggagc tttcataccc ggtatagctg
      601 aaaacggact tttcacgttc gagagagagt atgggaatga acgggttgtg gtgtgcttga
      661 acaactcgcc cgtcgccgga gaatacgaaa tcgggaagcc ttacgatgtt ctgctttcgg
      721 gatctgccag gatcgattca tttactctgt ctctagatcc gtacggtttc gcggtattga
      781 gaaagacggc ggaaaacgtc tgatctagat cgcaataaaa ccggcagtca tctggctgcc
      841 ggttttaatc gttgccggat ttatttcaat tctattctga cagacccttt cgtttctcca
      901 ccttccgata cgacgggaat gtttctcgag gatgagctgg cggttattct ggccgctccg
      961 atgaactccg cgctttcttc agtcactttc catatggata cggtatcgaa atccagaggc
     1021 caatcgactt caagctctgc gcgttgcccg ctagagatta tctcgtgaga gccggcgacc
     1081 agtttcactt tatcgatccc cgaagagaac aacgcgtatg ttatggaaac tacgcgatga
     1141 cccgcttcca cgaagatctt taaaggaacg gttaactcgc tctttccatc gctaaccttt
     1201 atcataacat ccttctgacc ggcctctcct gtgcgaggct cgaaaacgta tttaccgtcc
     1261 acgatgcttc caggcccttc tcgaatctca aacagcaaat catcgccgtc gggatcgctt
     1321 atgaaatccc cgagttcgat ttcaacggtc ttcccctctt cgagggtaac ttcgggaaac
     1381 ggttccttaa tttcgggtaa cctgttaaca tcgagaaccg ttatgatgat gggcagtgtc
     1441 acaccgccac ccctgccatc gctggcggtt acgagtatga atttttcgcc ggaatcctcg
     1501 taaccggggc tgtaggtgta gccgttccct tcaagtgttc ccggaccctc cagagagagt
     1561 gtcaattcat cgccgtcggg gtcgctgaag aaggtgttca ggttcatgcc aagggagaac
     1621 ccctctctta tgctgctctt gatctctttg atcgtagtct cgggggtgcg attcacatcc
     1681 accacttcta ccatgaattc gcccgtcacc gacgctagac catcggatac ctgtatagta
     1741 acagtgtggt ctccgctctc atcgaaagaa ggttcgaaag tgtagatcgc attttcgacg
     1801 ctcccgggtc cttccatgag agtgaaggtc agttcgtcgc cgtcgagatc tatcgactgc
     1861 atccggagat ccacaacgag gttagccccc tcgtctatca tcttatcgct gagaagtatt
     1921 ctgggggatc tgttaagatc catcacagag ataataatgg gtagatttgc ggagagaccc
     1981 gaggaatctc tcgcaactat cgttaccgtc ttttccccct ggtcgttgta cgaaggcgtg
     2041 tatttataat tacccccctc gatgcttccc ggtccttcta aaatggcgaa ggacagttcc
     2101 tcgccgtccg ggtcggtgaa gaagctcttg agatctagac ccagcgtgaa ggtctccttt
     2161 atgcttgccg caatcgaagg caggacagcc acgggagctc tatttacatc aacgacatct
     2221 atctcgaaga cgagtatctt ttcttcgcca ttggagtcgg agacctttat cttgacttcc
     2281 ttgagaccgg ccgattcaaa atccggctcg taggtgtaac tatcgccctc tacacttccc
     2341 ggtccttcaa gcagttcgaa gaatatccca tcgccgtcgg gatcgtaggc aaaggctttg
     2401 aggtaaaggt taagcctctc tccttcgaag atggtttcat tagctatcga tccttcgggt
     2461 ggtctgttcg catcctgcac caaaatcgtg aaagaagctc tgccagttcc gcctttgcca
     2521 tcggaaacca ttatagtaac ggtcttatca ccggcatcgc ggtagcctgg agtataaacg
     2581 tatctgttct cttgcaccgc gccgggccct tccacggtga tctctaaagg atcgttgtcg
     2641 ggatctatca ggcggctcga gaggtccact acgagttctt caccttcgga aataaccata
     2701 tcgctcaaga agattctggg cggtctgttc gtctcggtaa ccgtcacttt gaaagtcgag
     2761 gtcgattcgc ctcccctgct atctttggcg gagatcgaaa ccacactttc accggcctcc
     2821 gcaaagcccg gcttatagat gaagactccg tcgacgatct ctcccggtcc ttcgattagc
     2881 gtcagtgtca ccccgtcccc ttcagcatcc gaaacgagag ccgcgagatc tacggccaga
     2941 gagtctcctt cccggaccgt ttgatcggac accaccagag ccggcggcgc gttcacgtcc
     3001 accacttcga gttgcacatc cctcaccgtt tcgttgccct cggaatcttt tgcggtgatt
     3061 ctcaggactt tcttaccggc atccagatac ccgggagcat acacatacgt atttccctca
     3121 agctttccag gaccatcgac ggctagagac agctcatcgt tatcgggatc tttaacgata
     3181 gtcaaaaggt cgagggacat actctctccc tctaaaacct tgagcggtgt cgtgtcgatt
     3241 tccggaggtc tgttgactcg tatgacgttt atcttcagct ctccggtcga gtttttcccc
     3301 tgcttgtcgg tcgcctttat ctttgcgatc acttcgccca catacttgaa agccggctcg
     3361 aatgtgaata ccgatccggt tatggttccg gggccatcga ccttttcgag cgtgacttca
     3421 tcggcctttt catcttttat aaaggccctc aaatcgacag tgagcttttc tccctggttg
     3481 accacctgaa ccggcagctc cataacgggt tctttgttca gcagtgccgg aatcgtcgct
     3541 atgagaacga tcgccacagc gaccgcggcg atgagcgtat agatcatcca cttcttcatt
     3601 ttttctcccc cctcaacttt ttttatcaac caaagacctt ttatttctca aaacgtatat
     3661 cgctttttag atagttataa atcgcctgac tcagcgaatc gcggtatttg tgctctcccg
     3721 gaggctttgc ggtctttcca gctctttgaa aagcactacc acctcatcga accccattcc
     3781 caggagcaat ccgtggaaga agtttctggc gctctctctg gaagtctctt caatacccat
     3841 ttcgtgcgcg ctgatcaacg cctgttgttc tatctctttg ctgtaagact ggaacgtctt
     3901 gagcatatcc ctgttcccga aaaccttaac cagcacacca ctctgattta tcacacgtat
     3961 atccggtatc ttgctctggg gtatggtcac ctcgagctcc ggaggaggga gaactagctt
     4021 tattcttctg ctctcccctt cgtatatctc gatagagttg ctatctatgc tttttagatc
     4081 gagataaccg cgaacttgaa agggaactat aaaaatatat ctctgcgaag tgcccggcac
     4141 tatgctttcc ttttcaggtt cgaatatgag cagcgtggtc attctggccg tgctcagctc
     4201 ggcgattcgc tcgatcctgt gaatcaccga ttcggttctg acggtcgtct tcttgcgcga
     4261 ctgtaacagt atcagcagca gtatgcagac cgcgattatc agaacgatgt ttagaacgat
     4321 gagtaaaacc tccctgttgc catcttatgt gttatagaaa tcgaaattga aattgaaagc
     4381 cgcccccatt acgatcacgg cgatcaatgt gataagaacg aagacgatgg ccaggattat
     4441 aaggacgccc tggatcctga aatacttcat catgctgtcg agacctatct cgagattcac
     4501 aggagccttg gtgtgaatga aggtttccag ctccctcttc gttcttctta gcctgagacc
     4561 catcaccagc gcaacgatag cactgatgag accgaatata gaaaaggggt tcgctatcat
     4621 tcctgcgatc gagccgatta ttccgagtgt agcgccgatg agagtccata cgccgacgaa
     4681 tccggcccag tagctctaac cgctcagagt ctggtaagag attccttcga tgccagaatt
     4741 accttcgctt ctttcgtaca tacgaaacac cccctttttt attcacaaat aaagtttatc
     4801 agagaaagcg aaaaaaactg taatgctttg ccgagttggc gcttttgttg aactttcagc
     4861 aggggaagag tttcggtcga tatattgtta gttaagctta cggtggtata atcaaagcaa
     4921 acaaagtata tcggaattaa aatcattgag agtgaaggag gttatttatg gaatatcgca
     4981 aagttggaag aagcggcttg aggatcagtg aactgtctct cggttcgtgg ttgactttcg
     5041 gagcgcagct ggatatcgag aacgccaggg aggccatgcg cagagcatac agagagggaa
     5101 taaacttctt tgacaacgcc gaggcctacg cgagcggaat gtctgaatcg atgatgggcg
     5161 agatactaaa ggagttcaaa cgcgaggaag tcgtcgtttc taccaagatc ttctggggtg
     5221 gaaacaagcc gaaccagaaa ggcctttcgc ggaagcatct tctcgagggc acctggaact
     5281 ctctgaagcg actccaattg agctacgtcg atattctgta cgctcacagg ccagatc
//
LOCUS       DAOOLY010000171         5335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18530_length_5335_cov_4.545076, whole genome shotgun sequence.
ACCESSION   DAOOLY010000171 DAOOLY010000000
VERSION     DAOOLY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18530_length_5335_cov_4.545076"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..946)
                     /locus_tag="PLD15_08630"
     CDS             complement(89..946)
                     /locus_tag="PLD15_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB64013.1"
                     /translation="MTFNSDLLGRIQRESDDFTPTMKRIADAILENPSEAVNLSITQL
                     MNKSRVGSESTIVRFYRQLGFESFHEFKVTMAAEIAGSSFYHPYEDIRIDDSPGAVVK
                     KIFNGCIMALKENMEKISSQVITGAVELIEKSRRIICIGYGVSGSIANNLSFKLNLLG
                     KDAIYCPDSHTNAITLSKLKKDDCVFAISHSGESKDVVLPLQKVRNTVRIIALTGFID
                     STLAKIADLCIVTSVPEEMNIRTDAIVSRCVQMVVVDSLFACLAVREGEESLDTLSLS
                     RKSLSYLKF"
     gene            1214..1849
                     /locus_tag="PLD15_08635"
     CDS             1214..1849
                     /locus_tag="PLD15_08635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB64014.1"
                     /translation="MLTVYLMRHGQSQANVERIFANGDDGYPLTEEGRAQAALAAELL
                     ALKNIKRIYTSPILRAKQSAAIVEKRLGIEPVVLDEIREFSVGELEGQFISGEAESAF
                     MRLMIQWISGNSSDKIPNGESHLEIMARMKKAIDIIASENHTGEVLLVSHGGFLTMTL
                     PFICSGIDIREFFSRPSYGIGNCSITTVRVSNGAQSPSIELVEWANSSHLE"
     gene            1991..3949
                     /locus_tag="PLD15_08640"
     CDS             1991..3949
                     /locus_tag="PLD15_08640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPB64015.1"
                     /translation="MKKYTLKSGDIPTFVSEGLDEEQLKAVVESSARSLIVAGPGSGK
                     TRVITYKIAYLVSKGINPENILLVTFTRAASREMIERARRTSSSDLKGMLAGTFHHIC
                     NYFLRKYSAAAGLKENFTILDREDSKDLIKHCRTELLEERKVINTSTLPSAGVLQGIY
                     SYSVNVLASLRESITRQNRKFLGSYDEIEEIWKRYVREKAAQNCVDYDDLLLMALKLF
                     NENPSILTHESQRFKWILVDEFQDTNILQFRLVEMLSSVHGNLIVVGDDAQSIYSFRG
                     ARFENVYDFLNMKDSQIFKIQTNYRSTPQIVKLVNGIFPENSIEKELRAVRLDGPFPV
                     IAETWDNLEEASFVAQRIQEHINDGIDPDRIAVLYRSHFHSLELQMELDKRKMNYTLF
                     SGPRFTETAHVKDVLAIMKVIQNPLDQISWGRVLRLYPGVGNTTAFRIIQEISAAVND
                     GHLQVEVIRSHTSNRVRLENLIVLLEGLEPDALPAEVIRRFYESFYGDYLDEKHQDAR
                     ERRLDIERLMEIAGRYDSLADMLEDLAVSEKIDIEKESGERTPSIILTTVHQAKGLEW
                     DVVFVLAVNPGDFPNSMAIMEGSLEEEQRIFYVAATRAKDYLYIMRQKGGRSRPMIGN
                     RYVFRSGHDFIERIPGDTVERWDIGWDF"
     gene            4009..>5335
                     /locus_tag="PLD15_08645"
     CDS             4009..>5335
                     /locus_tag="PLD15_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPB64016.1"
                     /translation="MNALYLPLVAGIISTLFAFIMLKRTLGYSPGNDKMQQLSKYIQE
                     GASAFLEEEARKIFLVAVIIAAVLGVIFQSFKYPIALLFGALVSELAGVIGMYAATRA
                     NTRVAAGAEQGLSSAFKVAFSSGSVMGLAVAGFSLTGLALLMLIFKSSFLFENITDLS
                     RAFGSISYIDGVMIISSYSLGASMIALFDRVGGGMYTKAADMSADLVGKIEEHIDEDD
                     PRNPATIADNVGDNVGDVAGLGADILESYVASVVSAIVLAIFMKFADHGTMTEAQYYG
                     LILLPVLIAGVGVLASLVGVLVVANMKTKDAQKSLSFGNFFTGGLVLVSVAIVIGIAA
                     PDYPFKDSFIIGPGFVSKWRLFFAIASGLIAGIIISKLSEYYTSDQYKPTKKLAKDTQ
                     SGVAINITGGLALGMGSTIWPVVTLALAILGAYWSAGVYGIAIAALGMLS"
BASE COUNT         1433 a         1184 c         1319 g         1399 t
ORIGIN      
        1 gtcatccact tttcccgacg aatcgaacgt cgttagcact ggcgggataa ttcccttgaa
       61 aacagccatg ttgcacctcc tggtagaatt aaaatttcaa gtatgatagt gattttctgg
      121 ataaagacaa tgtatcgagc gattcttcgc cttctctgac agccagacaa gcgaagagag
      181 agtctacaac taccatctgg acacatctag aaactatggc gtcggttctt atgttcatct
      241 cctccggtac cgaggtaacg atacagagat cggctatttt agccagcgtc gaatcgatga
      301 agcctgtcaa cgcaattatg cgcaccgtgt tcctgacctt ttgaagggga aggactacat
      361 ccttgctctc cccggaatgt gagatggcaa aaacacagtc gtctttcttg agctttgata
      421 gagtaatcgc gttcgtatga gaatccgggc agtaaatagc atcttttccc agaaggttga
      481 gcttgaagga gaggttgttg gctattgagc cggaaactcc gtaaccaatg catattattc
      541 ttctgctttt ctcaatcagt tcgacggctc cggttatcac ctgactggag attttctcca
      601 tgttttcctt gagcgccatt atacaaccat tgaagatttt cttcaccact gctccgggtg
      661 aatcgtcgat tcttatatct tcgtacggat ggtaaaaaga ggaacctgcg atttcggcag
      721 ccatggtcac tttgaattca tgaaaactct cgaaacccaa ctgccggtaa aatctcacga
      781 tcgtagattc gctaccgacg cgtgatttgt tcataagttg agtaattgaa agattgaccg
      841 cttctgaggg gttttcgagt atcgcgtcgg caattctttt cattgtagga gtgaagtcat
      901 cgctttcgcg ctgtattctt cccagcagat cgctgttgaa tgtcacgact cacctccgaa
      961 aaaaatttct accataatat accatttaga gaaaaagtat tcaaatctat ctgttccata
     1021 tttaaggcaa ttaaagctat ttgagcttgt ttatagcctt ttaatctata aatgctgcct
     1081 tatcattaaa agaatttaat agaaaaaatt tctttctatc aattatgaag acccaatcag
     1141 aaacggtccg tgtgcagatt gcttttcagg ctgttatcat tagtagtgaa cgaagaaaac
     1201 tctggaggga attatgctga cggtatatct tatgcgtcat ggacagagcc aggcaaatgt
     1261 agagagaatc tttgccaatg gcgacgatgg ctatcctctg accgaggaag ggagagctca
     1321 ggccgctttg gcggctgaat tactagctct gaaaaatata aagagaatat acacttcgcc
     1381 aattctgagg gcaaagcaga gtgccgcgat agttgagaaa aggctgggca tagagcctgt
     1441 ggtgctggat gagataagag aattcagtgt cggggagctc gaagggcagt tcatttcggg
     1501 agaggccgaa tcggctttca tgaggcttat gattcagtgg atctccggaa actccagcga
     1561 taaaatcccg aacggagaaa gtcatctgga gataatggcc agaatgaaga aagccataga
     1621 tattattgca agtgaaaatc acactggtga ggttctactg gtgagtcacg gtggcttcct
     1681 gactatgact ctgccgttca tttgcagcgg gatagatatc agggagttct tctcgagacc
     1741 ttcctacgga atcggaaact gttcaattac caccgtgagg gtcagtaatg gcgcgcaatc
     1801 cccatcaatc gaactggtgg aatgggcaaa ctcctctcat ctggagtgaa agagaatttc
     1861 ccgtgatcct taacggacct gccagtctct taaaagttta aagccagaaa tgacttttat
     1921 cgcactgttc gacaatgaag gcgattcttg attgtggtaa aatttggcag aagggagtca
     1981 cgggtttgtt atgaaaaagt acacacttaa aagcggggac attccaactt ttgtgagtga
     2041 aggactcgat gaagaacaac tgaaggctgt cgtagaatcc tccgcccgct ctttgatagt
     2101 tgccggcccc ggctcgggca agacgagggt cattacttac aagattgcct acctggtcag
     2161 taaaggaatt aatcccgaaa atatactgct ggtaaccttt accagagctg cttctagaga
     2221 gatgatcgag agagccagaa gaaccagcag ctcggatttg aagggaatgc tagccggtac
     2281 ttttcaccat atttgcaatt acttcctccg aaaatactcg gctgcagccg gtctgaaaga
     2341 gaacttcact atactcgaca gagaagactc aaaggatttg ataaagcact gcagaacaga
     2401 acttctggaa gaacgcaaag taattaacac ttccacgtta ccatctgcag gtgttttgca
     2461 gggcatttat tcttactcgg tgaatgtcct ggcttcttta agagaatcga tcacgagaca
     2521 gaacaggaaa tttctaggct cttacgacga gatcgaggag atctggaagc gctatgtcag
     2581 ggagaaagca gcccagaact gcgtcgatta cgacgatctt ctactgatgg ccctgaagct
     2641 tttcaacgag aatccatcaa ttcttaccca tgaatcgcaa aggttcaaat ggatactggt
     2701 cgatgagttt caggatacca atatacttca attcaggctg gttgaaatgc tttcctcggt
     2761 tcacgggaac ttgatcgtag tgggtgacga tgcccagtct atctactctt tcagaggagc
     2821 cagattcgaa aacgtttacg attttttgaa catgaaggat tctcagatct tcaaaataca
     2881 gaccaactac cgctctacac cgcagattgt gaaattggtc aatgggatct tccctgaaaa
     2941 ttccatagag aaggaactca gagccgtgag gcttgatgga ccgtttcctg taatagctga
     3001 gacctgggat aacctcgagg aggcctcttt cgtagcccaa cgtattcagg aacacataaa
     3061 cgacgggatc gatccggata ggatagccgt tctatacaga tcgcactttc actcgcttga
     3121 actccagatg gaactggaca agagaaaaat gaactacact ctcttctccg gtcccagatt
     3181 caccgaaaca gctcatgtga aagatgtact ggcaatcatg aaagttattc agaacccgct
     3241 ggatcagatt tcctggggaa gggtactcag actctacccc ggagtgggaa ataccacagc
     3301 cttccgtata atacaggaga tatccgcagc cgtaaacgat ggccatcttc aggttgaagt
     3361 tatcaggtct catacgagca acagagttag acttgaaaac ctgatcgtcc ttctcgaagg
     3421 tcttgaaccg gatgcactcc ccgcggaagt cataaggagg ttctacgaga gcttttatgg
     3481 ggactatctg gatgaaaagc atcaagacgc gagagagagg cgcctggata tagaaagact
     3541 tatggaaata gccggtaggt acgattcgct tgccgatatg ctcgaagatc tagctgttag
     3601 cgagaagata gatatcgaga aggagtccgg ggagcgcacc ccttctataa ttcttactac
     3661 cgttcatcag gcgaagggac ttgaatggga tgtggtcttc gttctggcag ttaatcccgg
     3721 tgattttccc aactctatgg ccataatgga aggttccctt gaagaagagc aaaggatatt
     3781 ctatgttgct gcaaccaggg cgaaggatta tctgtacata atgagacaga agggcggcag
     3841 atcgcgtccc atgattggca acagatatgt attcaggagc gggcatgact tcatcgagag
     3901 gattccgggc gacaccgtcg agcggtggga cataggctgg gatttttgat tttttatttt
     3961 gtcgtcttat agatcgcttc acatagtcga aacatgggag ggaaacagat gaatgctttg
     4021 tacttaccac tggtagcagg tattatctct acgttattcg cgtttattat gctgaagaga
     4081 actctcggct actctccggg caatgacaag atgcaacagc tttccaagta cattcaggaa
     4141 ggagcgtcgg cttttctaga agaggaagcg agaaagatat ttctggtggc cgtcataata
     4201 gccgccgttc tgggagttat cttccaatct ttcaagtatc ctatcgcatt gctatttgga
     4261 gctcttgtct cggagttggc tggagttata ggtatgtacg ccgccacgag agccaacacc
     4321 agggtcgcag ccggggcaga acagggtctt tcgagtgctt tcaaagtggc cttttccagc
     4381 ggttcggtca tgggcctggc tgtcgcgggg ttctctttaa ctggcctggc cttgcttatg
     4441 ttgattttca agagctcctt cctgttcgag aacataaccg acctttcgag agccttcggt
     4501 tcgatctcct atatagatgg agtcatgatt atcagctcct attcgctcgg tgccagtatg
     4561 atcgcgctct ttgatagagt cggtggcggt atgtacacca aagctgcaga catgagcgcc
     4621 gatctagtcg gtaagataga ggaacatata gacgaggacg acccgagaaa tcccgcaact
     4681 atcgccgaca acgttggaga caacgtagga gacgtggccg gactcggggc ggatattctt
     4741 gaatcctatg tggcctcggt cgtttcggca atagttctgg ccatcttcat gaagttcgcc
     4801 gatcacggca caatgaccga agcacagtat tacgggttaa tacttcttcc tgtccttata
     4861 gccggtgtcg gcgttctggc atcgctcgta ggggttctgg tcgtggccaa catgaaaaca
     4921 aaggatgcac aaaaaagcct cagtttcggg aatttcttca ccggtgggct cgttctggta
     4981 tcggtggcaa tcgtcatagg catagcggca ccggattatc ctttcaaaga ttccttcatc
     5041 atcggtccgg gtttcgtttc gaaatggaga ttattcttcg ccatcgcctc gggccttatt
     5101 gcaggtataa taatctccaa gctcagtgaa tactacactt ccgatcagta caaacccaca
     5161 aagaaactcg ccaaagacac ccagagcggt gtcgcgataa acatcaccgg tggtctggcg
     5221 ctgggtatgg gaagcactat ctggccggtc gtaacgcttg ccctcgcgat tcttggagct
     5281 tactggtcgg ccggagttta cggaatcgcc atagctgcgc tgggaatgct gtcgt
//
LOCUS       DAOOLY010000172         5320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18602_length_5320_cov_4.940171, whole genome shotgun sequence.
ACCESSION   DAOOLY010000172 DAOOLY010000000
VERSION     DAOOLY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18602_length_5320_cov_4.940171"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLD15_08650"
     CDS             <1..447
                     /locus_tag="PLD15_08650"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPB64017.1"
                     /translation="HPIARGNKGLFLGGVKVSDEFYLHGHSDGDVLSHAIVDAILGAA
                     GAGNIGLWFPENEVNRGRRSIEFLAEVRTGLTDSWEIVNIDSVVVIGEVRLSGRIAEI
                     EESIAQALKVSSEIISVKPKSGNGGGTQFVQSQAICLLRRVGNVDA"
     gene            440..1639
                     /locus_tag="PLD15_08655"
     CDS             440..1639
                     /locus_tag="PLD15_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPB64018.1"
                     /translation="MPDFRYEVINSKGKPEKGKLTASSKLDALQILSGRGYTVSAIRP
                     IASQDKVRTSIFPLSPKEVSLFARQLSTMVSSGVRIRDALQVLSTQALFSRRFRRVIT
                     RVVLDIESGMSFNESLEKSGVFEPLFTNLVKAGETGGVLDESLQRVSEFYEGMVELQN
                     EVKSAMAYPLFMMVFAVGIVGIISFFILPNLISAFGGNFKPSGVMGILLSVNDILKSH
                     WPFILIGLVVVLIGGWLFFKSKYGNYVKEIFGKIIPPIRKLRNMTAMERFTRTTAVLI
                     ASGVDLPTVLELAGQVSQSPSLEHAVQISISQIKAGESIHVALAQQKVFPAIVVSMVA
                     TGEETGKLDEVMFKVADFYHTQVQSSLKKLVSLVEPIMIMFIGGFIGFLAYTMYNTIF
                     AMEQSIG"
     gene            1636..2016
                     /locus_tag="PLD15_08660"
     CDS             1636..2016
                     /locus_tag="PLD15_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64019.1"
                     /translation="MSVVELVCCLAIVGICMGCLMAFSGEMISNLSIELSKVELDCFL
                     ENQRLEAVRRSKTVELIYNYRQRKLFSNTGDVLEGCLWNNLEDFRIIFNGEGSIAILS
                     GSTTLCFENGTVLTIQPVTGKVTY"
     gene            2042..3079
                     /locus_tag="PLD15_08665"
     CDS             2042..3079
                     /locus_tag="PLD15_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64020.1"
                     /translation="MFQTMITALNVDDGYTEIARAVVEGRYLKPVFSVLKTGPKSLDE
                     AVRIFMQYNALPSDEIFVTSFHPKNVVFQHLEVPKVKSMKNLLSVASYKAASQFSLPP
                     DEVSVACLNSLTALKSGIVPAFVITRRKVLDEFISNLRQSGFPEPNVVDVKPFQIFKI
                     AQLDALEGNYILFTVDSDYSLLVMFKGDEIVGLNYINGGFSDVADKFKEDDSLSPNTD
                     IQREFLTGSKAYYDSLIEEASEQLENMISYQLRMYLTNTLSSSPNTSAADEASFSKFF
                     VVGQSSLSTAVYGRAFQRMLGGDAGVLVLPLKIKEAIGITYTTAGLLMRGGEQLGRRK
                     LVFKEETSLQA"
     gene            3087..3599
                     /locus_tag="PLD15_08670"
     CDS             3087..3599
                     /locus_tag="PLD15_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64021.1"
                     /translation="MIILLLIVVLVMGARFLASQAFEFRLRQISRLKGYLFDNTGTYL
                     TGNLRQDIQTIRDKKQEYINSKNRLSIQVRDLRTHVQGKSLEMNIFRTLNAFLEDESA
                     IKTILTRMDFENGKGNAFYYLIYDGNTVSFPTPSSTPVLLSTPNVRGNLEFADLYTLQ
                     IIDLEVGDEQ"
     gene            3596..4201
                     /locus_tag="PLD15_08675"
     CDS             3596..4201
                     /locus_tag="PLD15_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64022.1"
                     /translation="MSKNDSDDLDFKEIEFSDRSVTGVPESSEFFIHKDFKKRLSRKL
                     TFLDRKGINAKLLLGALVLIAVAGFSVLVVIYSYNAFKNTATLLAEVNELENAFLEVQ
                     LLTRVRNETIGSMSTRSYLGDLQKILGISNKPGDYRGISLSGRSRDILDLLEMIVNEP
                     RMMIKKLELRSNLGFPILPESTLPSNIMIELRTDVSITGSF"
     gene            4217..4657
                     /locus_tag="PLD15_08680"
     CDS             4217..4657
                     /locus_tag="PLD15_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB64023.1"
                     /translation="MEKMVVIIVVLICALVTGGVLLAFFLQPVEITEVTANRIRVEPI
                     NTSSLIEPVKMQDHFTPLFASPSEVISSMLEVGQEAENISYLAYFRLNRVDYAVLDTA
                     GTQVTVRVGERVDPSYIVYGITEFAVLLNDTSTNSFVVVKYFRS"
     gene            4695..>5320
                     /locus_tag="PLD15_08685"
     CDS             4695..>5320
                     /locus_tag="PLD15_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64024.1"
                     /translation="MRKVAVLFVVLSAIALAFSNQLGSVIPSMDVESVTLTLVFNEPF
                     VQSYNIQSNPSKTIYSLSLNGVNGKDMNLPLRMGPVEGLWIRQEPSRLSINTALLTPA
                     RVEPEIIIRENVVIIRFFRSSAEVDIDKFTTYGMTIASAMTYLFSEEILNLSYILSPS
                     VRDEKILVGFNLALPEEILRNILTSLGDRVAYAYLSDGTFYLGTPDEV"
BASE COUNT         1402 a         1182 c         1352 g         1384 t
ORIGIN      
        1 catccaatag cgagaggtaa caaagggctt tttcttggtg gagtgaaggt atccgacgag
       61 ttttatctcc acggtcattc cgatggcgat gtcttatccc atgcgattgt tgatgctatt
      121 ctcggagcgg ccggtgccgg caatatagga ctctggtttc ctgaaaacga agtgaacagg
      181 ggtaggagaa gcatagagtt tctcgccgaa gttcgaacgg gactgacgga ctcatgggag
      241 atagtcaaca tcgattcggt ggtggtgata ggagaggtga ggttatccgg gcgcatcgcg
      301 gaaatcgagg aaagcatcgc gcaggcgttg aaggtttcaa gtgaaatcat aagcgtaaag
      361 ccgaaatccg gtaatggagg cggcactcaa ttcgtacagt cacaggcgat ctgtcttctc
      421 agaagggtgg gaaatgtcga tgcctgactt tagatacgaa gtcataaaca gtaagggtaa
      481 gcccgaaaaa ggaaagctga ccgccagctc caaactcgat gcccttcaaa ttctatcggg
      541 caggggttac acggtgagcg ccataaggcc tatcgcatct caggacaaag tccggacctc
      601 gatctttcct ctgtctccga aagaggtaag tctctttgcc aggcaactat ctaccatggt
      661 ttcttcgggg gtcaggataa gagatgcgct tcaggttctt tcgacccagg cgctcttcag
      721 tcggaggttc aggagggtta tcaccagggt agttcttgat atagagagcg gtatgagttt
      781 caacgaatcg cttgaaaaat ccggagtctt cgaacctctc ttcaccaacc tggtcaaggc
      841 cggggagacc ggcggtgttc ttgacgagtc gctccagaga gtttcggagt tctacgaagg
      901 gatggttgaa ttgcagaacg aagtaaaatc cgcaatggcc tatcccctgt tcatgatggt
      961 tttcgcggtc ggtatcgtgg gaataatatc ttttttcata ctgccaaacc ttatcagtgc
     1021 cttcggtggt aatttcaaac catcgggcgt gatgggaatt cttctgagcg taaacgatat
     1081 tcttaagtcc cactggcctt tcattctgat tggactggtc gtcgttttga tcggtggatg
     1141 gctcttcttc aaaagcaaat acggcaacta cgtcaaagaa atctttggaa aaatcatacc
     1201 tcctataaga aagttgagga acatgacggc catggaaaga ttcaccagaa cgaccgcagt
     1261 tctcattgca agcggtgtcg atctgccgac cgtactggaa cttgccggtc aggtctctca
     1321 aagcccctct ctagagcacg ccgtacagat ctcgatctcg caaatcaagg ccggcgagag
     1381 tattcacgtt gccctggccc agcagaaagt ctttccggcg attgtggtga gtatggtcgc
     1441 aacgggcgag gagaccggaa agctcgacga ggttatgttt aaggtggccg acttctatca
     1501 cacacaggtt cagagttcgc tgaagaaact ggtttcactt gtcgagccta taatgatcat
     1561 gttcatagga ggattcatag gttttctggc ttacaccatg tacaacacga tattcgcaat
     1621 ggagcagagt attggatgag tgtagtagaa ctcgtatgtt gtctggccat cgtcggcata
     1681 tgtatgggtt gtctcatggc tttctcggga gaaatgatct ccaatctgtc gatagagttg
     1741 agcaaagttg agctcgactg ttttctcgaa aatcagagac tggaggctgt cagaagatcg
     1801 aagaccgttg agttaatata taactacagg caacggaagc ttttctcgaa caccggagat
     1861 gtgctcgaag gttgtctgtg gaacaatctg gaggacttca ggataatctt caatggcgaa
     1921 ggttcgatag cgatactcag tggctcaaca acgctgtgct ttgagaacgg tactgttttg
     1981 acgatacaac ccgtaactgg gaaagtcaca tactgagcaa cgatccgggg agtgaagcca
     2041 aatgttccag accatgataa cggcactgaa cgttgatgac ggttataccg aaatagccag
     2101 ggctgtagtc gaaggtcgtt acttgaaacc tgtcttttcg gttctaaaaa ccggtccgaa
     2161 gagtctggat gaagcggtca gaatctttat gcaatacaat gcccttccct ccgacgaaat
     2221 cttcgtaacg agttttcatc caaagaacgt cgttttccag catctggagg ttccaaaggt
     2281 caaatccatg aagaaccttc taagcgttgc gagttacaaa gcggcttccc agttctccct
     2341 accaccggac gaggtcagtg tggcatgcct taattctttg accgctctta aaagtggaat
     2401 cgtaccggct tttgtcatta cccgaaggaa ggtcctcgat gagttcataa gcaatctcag
     2461 acaaagcggc ttccccgaac ccaacgttgt tgatgtgaag ccttttcaga tcttcaaaat
     2521 cgctcagctg gatgcgctcg agggtaacta cattctcttt acggttgaca gcgattactc
     2581 tctcctggtt atgttcaagg gcgatgaaat agtgggattg aactacataa acggcggctt
     2641 ctcggacgtg gcagacaaat tcaaggaaga tgattctctg tcaccgaaca cggatattca
     2701 aagggagttt ctcacagggt ccaaggctta ctacgattca ctcatcgagg aggcctccga
     2761 acagcttgaa aacatgatat cttatcagtt aaggatgtat ttgaccaata ccctatcgag
     2821 ttccccgaac acctcggccg ccgacgaggc atctttcagt aagtttttcg tggtcggtca
     2881 gtcgagcctt tctacggccg tttatggaag ggctttccag aggatgctcg gcggggacgc
     2941 cggtgttctt gtcttacctt tgaagataaa ggaagcgatc ggaattacgt acactacggc
     3001 cggacttctc atgaggggtg gtgagcagct tggtagacgt aaacttgtat tcaaagaaga
     3061 aacgagtttg caggcttaga aatgctttga tcatactgtt gctcattgtt gttctggtta
     3121 tgggagcgcg ctttttagcc tcacaggctt tcgaattcag gttgaggcag atctcgaggt
     3181 tgaaaggcta tctcttcgat aacaccggaa catacctcac aggtaacctc aggcaggata
     3241 ttcaaacgat aagggataaa aagcaggaat acatcaactc aaagaacaga ctttcaattc
     3301 aggtcagaga tcttagaaca catgttcagg gcaaatccct tgaaatgaat atcttcagaa
     3361 ctttgaacgc ctttctggaa gatgagagcg cgatcaagac tatcctcacc agaatggatt
     3421 tcgaaaacgg caagggtaac gctttctact atctgatata cgatggaaac acagtctcct
     3481 tccctactcc atctagtact ccggttttgc tgtcaacccc caacgtacgg ggcaacctgg
     3541 aattcgcgga tctttacact cttcagatca ttgaccttga agtgggtgat gaacaatgag
     3601 caagaacgat agcgacgatc tggatttcaa agagatagag ttctccgatc gatcagtaac
     3661 gggagtcccg gagtccagcg agttttttat tcacaaagat ttcaagaaac gcctcagcag
     3721 gaaattgacc ttcctcgaca gaaagggtat caacgcaaag ctcctgcttg gagctctggt
     3781 gcttatagcc gtggccggtt tctccgttct ggttgttatc tattcgtaca acgctttcaa
     3841 aaatacggct actcttctgg cggaggtgaa cgaactcgag aacgcttttc tcgaggtgca
     3901 gcttctcacg agggttagaa atgagacgat cggctccatg agcacgagga gttatctcgg
     3961 cgatctccag aaaatcctgg gtatttctaa caaaccggga gattacaggg gaatttcctt
     4021 atccggcagg tcgagggaca ttctcgatct tcttgagatg atagtgaacg aacccaggat
     4081 gatgataaag aagctagaat tgagatcaaa tctgggtttc cctatacttc cagagtcaac
     4141 tctgccatcc aacattatga tagaattgag aacagatgtc tctatcaccg gaagttttta
     4201 ggataggatt gatggtatgg agaagatggt tgttataatc gtcgttttga tatgcgcctt
     4261 ggtaactggt ggtgtactgc tagcattctt tcttcaacct gtggaaataa ccgaagtcac
     4321 ggccaataga ataagggttg agccgatcaa cacttcaagt ttgatcgaac cggtgaagat
     4381 gcaagatcac ttcactccgc tttttgccag cccgtccgag gttataagct ccatgctcga
     4441 agtggggcag gaggcggaaa acatatctta tttagcgtat ttcagattga acagggtgga
     4501 ttacgccgtt ctcgacacgg ccggaacaca ggtcacggtc agggttggag aacgtgtaga
     4561 tccctcgtat atagtgtacg gaataaccga atttgccgtt ttgcttaacg ataccagcac
     4621 gaacagcttt gtggtcgtga aatatttcag gtcttgaact cctctgactc tagggtgaat
     4681 atggggtgat ttctgtgaga aaagtcgcgg ttctcttcgt ggttctgagt gccatcgctt
     4741 tagcattttc gaatcaactg ggttcggtga ttccttcgat ggatgttgaa tccgttactc
     4801 tgacgcttgt cttcaacgaa ccgttcgtcc aatcgtacaa catccagtca aacccatcga
     4861 agacgatata ctcgctttct ctgaacgggg tgaatggaaa ggacatgaat cttccgcttc
     4921 ggatgggacc tgttgagggt ttgtggatca ggcaggagcc gtcacgactc tccattaata
     4981 ccgcattgct cacacccgcc agagtcgagc cggaaatcat cataagagaa aatgttgtga
     5041 tcataaggtt tttccgatcg agcgcggagg tggatataga taaattcaca acttacggaa
     5101 tgaccatcgc cagcgcgatg acctatctct tttcagagga gatattgaac ctttcttaca
     5161 tactctcacc aagcgtgaga gacgagaaga tacttgtagg cttcaacctg gctctaccag
     5221 aagagatact tcgaaacatc ctaacctcgc tcggtgacag ggtggcatac gcatatttga
     5281 gcgacggtac tttttatctg ggtactcccg atgaagtgag
//
LOCUS       DAOOLY010000173         5279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18774_length_5279_cov_4.528905, whole genome shotgun sequence.
ACCESSION   DAOOLY010000173 DAOOLY010000000
VERSION     DAOOLY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18774_length_5279_cov_4.528905"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1171)
                     /locus_tag="PLD15_08690"
     CDS             complement(2..1171)
                     /locus_tag="PLD15_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB64025.1"
                     /translation="MRYQLECSKLEFFRPDAFAFINKLKTQPSKYPMINLSIGAPNRP
                     TPAWIIEAMKTHLDNPAYHTYPPQHGAPELCEAVAFWYGKRFGVEIDPKDNVLVTVGI
                     KEAIFNALHSLVNAGDVVMVPDPGYPTYFEAVLFTGGKLLTYNSDAESDRVLNEIEQK
                     ARLHGPKIVIVNYPSNPTGRIVGLDFYKSLSEIASRYDFAVMSDLAYSEIAFDGLTVH
                     SYLEARPGLELGFEYFAFSKTYNMAGWRVGAIVGDKRLVDAVKLYKSKIDSNVFYPIQ
                     QAAATALIETPDSFYRELSGMYQQRRDALVHGLSDIGLRFSPPQGAMYTWVETPEGID
                     PWLFTEKLYKEYGILVVPGTAYGLNGGQRVRMGLVQETPLMEEAARRLAEGGLMR"
     gene            complement(1168..1812)
                     /locus_tag="PLD15_08695"
     CDS             complement(1168..1812)
                     /locus_tag="PLD15_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB64026.1"
                     /translation="MQDISLMQQLSRLGIAFLSNLAFLPFVLAIGIILGIVIAFVRFH
                     RIPVLSQTLTVLVEIVRGSPFLIIVYAIYFALPYVGIELGAFQTGIVVLSITATAYLS
                     EVFRSGLVAMDKGQFEAAEALGMSYFKKVTLIILPQIIKTTMPSIVGQIVMTIKDTSI
                     VSLVGMVEIVRTSRQIMQLTLNPFMAFSIVSVFFILVCYPLIIVSKKLEGGNNR"
     gene            complement(1812..2492)
                     /locus_tag="PLD15_08700"
     CDS             complement(1812..2492)
                     /locus_tag="PLD15_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB64027.1"
                     /translation="MRLISQYLPEFFEALFETLKVAALSGVFALIIGTVVGIISTMKS
                     KLAIIPVGIYTGFIRSTPLLVQLYFVHYGLPITGIMLTSFQSAIIAFSLNSGAYISEI
                     VRGGLASMDKGQSEASRALGLSWFQTMRLIILPQVFKKILPPLISQFSYLIKDTSLAA
                     VLVIPELTYTARKIAARTYMPFESFGVPMLMYFGIYLILALLSRFISGKKEPGRSARR
                     WFGFGKVV"
     gene            complement(2510..3241)
                     /locus_tag="PLD15_08705"
     CDS             complement(2510..3241)
                     /locus_tag="PLD15_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPB64028.1"
                     /translation="MERVVRIRGLHKSFGKLHVLKNIDLDVYESDVLVLCGPSGAGKS
                     TLLRCINMLEHYHEGTIDVLGKDIKSSRANLSYVRKNSGMVFQHFNLFPHISVMDNVA
                     LAPIHVKKIPKDQAYEKAKGLLDQVGLSAKYDAYPGQLSGGQKQRVAIARALAMDPKL
                     MLFDEPTSALDPEMIKEVLDVMIKLALGGMTMIIVSHEMGFAREVANKIGFLDDGKII
                     ELTPKNEFFTNPRSERTKEFLSKIL"
     gene            complement(3313..4098)
                     /locus_tag="PLD15_08710"
     CDS             complement(3313..4098)
                     /locus_tag="PLD15_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64029.1"
                     /translation="MKKLLVLVVIAVLLVSSTMAVTALENIVKKGKIVVATDMTAVPM
                     QYRDPAGKPTGFTVELMELAAREMGVQIEWQDMAWESLIPSLLSGKVDMIAANMSMTL
                     TRMKSIRFSDPYFLTGIVAIARKDSPLVSWKDLAAPSVKMGATMGSVHADFIEQTWGK
                     KASLYDNLAEWMTDLKLGRIDSVMDDEMICIELANKNPELKILEGYVRPDTYGLAFRQ
                     DQDSDSLVNWFNWFIKWEKLTGEYGKIYEKYVGKEWVPSFVVD"
     gene            complement(4406..5161)
                     /locus_tag="PLD15_08715"
     CDS             complement(4406..5161)
                     /locus_tag="PLD15_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPB64030.1"
                     /translation="MTEHFKAFKKVDEIFELFFEKNKWRFSDMVARLGYPKATVHNIL
                     AAMTNLDYLARDGNNYMLGNRILQLSGLMRRNMEFREIALPHLEELRNRINETIHLTS
                     LYRDQVIYIECLHPTHALRPHSTVGVTASLYCTGVGKALLAFQSDSYINGYLERVKLV
                     KRTPNTITDPELLKKEIDTIRCNGYSYDRIEQEESIKCIAAPILDNHGVARYAVSIAG
                     PADRMTDEAIEAYKEPFLRTMKILNDLYRDFAN"
BASE COUNT         1237 a         1357 c         1281 g         1404 t
ORIGIN      
        1 ctcatcgcat taatcctcct tctgccagcc tccgggctgc ctcttccatg agcggcgttt
       61 cctgcaccag tcccattctc accctctgcc ccccattgag cccgtacgcc gttcccggca
      121 caacgagaat tccgtattct ttgtatagtt tctcggtgaa gagccaggga tcaatgcctt
      181 caggggtttc cacccaggtg tacatggcgc cttgcggcgg agagaatcta aggcctatat
      241 cgctcaatcc gtggacgaga gcatcacgcc tttgctgata cataccgctc agttctctgt
      301 aaaaagaatc gggcgtctct atcaaagccg ttgcagccgc ttgctgtatc ggatagaaga
      361 cgttcgagtc gatttttgat ttgtacagct tcaccgcgtc gaccagcctc ttgtcaccga
      421 cgatggcgcc caccctccag ccggccatgt tataggtctt cgagaaggcg aagtattcga
      481 atcccagttc caggccggga cgggcctcca gataactgtg gaccgtcagc ccgtcgaaag
      541 ctatctccga gtacgccaga tcgctcatca cagcgaagtc gtagcgtgag gctatttccg
      601 agaggctctt atagaaatcc agaccgacaa ttctgcccgt gggattcgat gggtagttca
      661 cgataacgat cttcgggccg tgaagtctgg ccttttgttc gatctcattc aaaaccctgt
      721 ccgactcggc atcgctgttg taggtcaaca acttgccccc ggtgaacaga acggcctcga
      781 aataagtcgg gtagcctgga tctggtacca tgacgacgtc tcctgcatta accagcgagt
      841 gcaaggcgtt gaatatagct tcctttatac ccacggtgac gagtacgttg tctttggggt
      901 ctatctcgac tccaaaacgc ttgccgtacc agaaagccac cgcttcgcag agctcgggag
      961 cgccgtgctg gggcggatac gtgtggtagg ccggattgtc gagatgcgtt ttcatggcct
     1021 cgattatcca ggcgggggtg ggtctgttcg gagcaccgat ggaaaggttg atcatggggt
     1081 atttggatgg ttgtgttttc aacttgttta tgaaagcaaa agcatccggc ctgaagaact
     1141 caagctttga gcactccagt tggtatctca tctgttatta cctccctcga gttttttcga
     1201 aacgattatc aacggatagc atacgagtat gaaaaaaacc gatacaatgc tgaaagccat
     1261 gaacgggttc agcgtgagct gcatgatctg acgggacgtt ctgactatct ccaccatccc
     1321 taccagcgag actatagatg tgtcctttat tgtcatcacg atctgaccga caatcgaagg
     1381 catcgtcgtc ttgattatct gtggcagtat tatgagcgtg acctttttaa agtagctcat
     1441 accgagcgct tcggcagctt cgaactgccc tttgtccata gccacaagac cgcttctgaa
     1501 aacttccgag aggtaagcgg tcgccgttat agaaagaacc actattccgg tctgaaaggc
     1561 tcccagttca attccgacat atggcagggc gaagtaaatg gcgtaaacga ttatcaggaa
     1621 gggagagccc ctgacgatct cgaccagtac ggtcagggtt tgcgacagta cgggtattct
     1681 gtgaaacctt acaaaagcta tcactattcc cagtattatt cctatggcga gaacaaaggg
     1741 cagaaaggcc aggttgctca gaaaggctat gccgagcctg gaaagctgct gcatgagcga
     1801 tatgtcctgc atcataccac cttcccgaag ccgaaccagc gccttgcact acgcccgggc
     1861 tctttcttgc cgctgatgaa acggctcaaa agcgcgagta taagataaat gccgaagtac
     1921 atgagcatcg gcactccgaa cgattcgaag ggcatatacg ttctcgcggc tatctttctg
     1981 gccgtgtagg tgagttccgg tatcacgagg accgcggcca gactcgtatc ctttatgaga
     2041 tacgagaatt ggcttatcaa cggcgggagt atcttcttga acacctgggg aaggattatc
     2101 aacctcatgg tttgaaacca tgaaagcccc agcgccctcg aagcttccga ttgtcccttg
     2161 tccatagaag cgaggccacc cctcactatc tcggatatgt acgccccgct gttgagagag
     2221 aatgcgatga tagcactctg aaaagaggtc aacatgatac cggttatggg aagcccgtaa
     2281 tgcacgaagt agagctgaac gagcaaaggt gtggatctta taaaacccgt gtaaattcct
     2341 acaggaataa ttgccagctt tgatttcatt gtcgatatta ttccaacgac ggttccgatt
     2401 atcaaagcaa aaacgccgct gagtgcggct accttgagag tttcgaacaa cgcctcgaaa
     2461 aattccggca gatactggga tatcaatctc atatcaagca cggtatcacc tacagtatct
     2521 tgctcaggaa ttctttggtc ctttcactcc tggggtttgt gaagaactcg tttttcggtg
     2581 taagttcgat tatctttccg tcgtctagaa agcctatctt gttggccact tctctggcga
     2641 agcccatctc gtggctgact attatcatcg tcatgccgcc gagcgccagt tttatcatga
     2701 catcgagaac ttcctttatc atctccgggt caagagccga agtaggttcg tcgaaaagca
     2761 tcaattttgg atccatggcg agagctctgg cgatcgccac tctctgtttt tgaccgcctg
     2821 atagttgtcc cggatacgcg tcgtatttgg ccgagaggcc tacctggtcc agtaaaccct
     2881 tcgccttttc gtacgcctga tctttcggaa tcttcttgac gtgtatcggc gccagagcga
     2941 cgttgtccat caccgaaatg tgaggaaaga gattgaagtg ttgaaaaacc atgccggagt
     3001 tcttcctgac atagctgaga ttggcgcgac tggacttgat gtcctttcca aggacatcga
     3061 tcgttccctc gtgataatgc tcgagcatgt taatacacct gagcagcgta ctcttgcccg
     3121 cgccgctcgg gccgcagaga accagcacgt cgctttcgta aacatcgagg tctatgtttt
     3181 ttagcacatg gagttttccg aatgatttgt gtaagcctct tatccttaca acacgctcca
     3241 tagaattccc ctttcaattc ctgaagtggg atggggagga ccccatccca cagaataatg
     3301 tttccagaac gctcagtcaa ccacgaacga aggaacccat tctttgccga catacttttc
     3361 atatatcttt ccgtactcac ctgtcagctt ttcccacttg atgaaccagt tgaaccagtt
     3421 aaccagcgaa tcggaatcct ggtcctgtct gaaggccagt ccgtaagtat cgggtcttac
     3481 ataaccttcc agaatcttca gctcggggtt cttgttcgcc aattcgatac agatcatttc
     3541 gtcgtccatg accgaatcga ttctgccgag cttgagatcg gtcatccact cggccaggtt
     3601 atcgtacaga gaggccttct tgccccaggt ctgctcgatg aaatcggcgt ggaccgatcc
     3661 catggtcgcg cccatcttaa cgctgggagc cgcgagatcc ttccagctga caagcgggga
     3721 gtccttcctt gcgattgcca cgattccggt caggaagtac ggatcggaaa aacggatcga
     3781 tttcattctg gtgagggtca tcgacatgtt cgcggcgatc atatcgactt ttcccgagag
     3841 gagcgaagga ataaggcttt cccaagccat atcctgccat tcgatctgga ctcccatttc
     3901 cctggcggcc agttccatca attcaaccgt gaatccggta ggtttcccgg cgggatctct
     3961 gtactgcatg ggaacggctg tcatatcggt ggccacaacg atttttccct tcttcacgat
     4021 gttctccagc gccgttaccg ccatcgtgga gctcacaagc agaaccgcaa taactacaag
     4081 aacaagcaac ttcttcattt tttcttcccc tttcgtcttt tagtatgctt accgtcacgg
     4141 taagtgaaaa ctcatcacgg attgaaaaca gacatagttt actggaaatt tgttcaataa
     4201 tatagaacac tattcaacaa tgtagtaatt catgtaaaaa cgataccaga taactcccga
     4261 taaaacaaaa acatcctggt ctgatttatg acattctaaa acaatgtagg gtgtttatac
     4321 ctgattatga agtgcaattt gatttcactg actgtggatg agtgcttttc gaccgctagc
     4381 tttatgaatc tcgagtttaa ggagattagt tggcgaagtc tctataaaga tcgttgagaa
     4441 tcttcatcgt tctcaggaaa ggttccttgt aggcttcgat cgcttcgtcg gtcattctgt
     4501 ctgccgggcc ggcgatgctc acggcatatc tggccactcc atggttatcc agtatgggag
     4561 cggcgatgca ttttatgctc tcctcctgct cgatcctgtc gtaggagtat ccgttgcagc
     4621 gtatcgtgtc aatctctttc ttcaacagtt ccgggtccgt gattgtgttt ggcgtcctct
     4681 tcaccagctt aaccctttcc agatagccgt ttatatatga gtcggactga aaagccagca
     4741 gggctttgcc gactccggtg cagtaaagcg aggcggttac gccgacggtc gaatgcggcc
     4801 tcaaggcgtg ggtgggatgg aggcattcga tgtatatcac ctgatctctg taaagcgatg
     4861 tgaggtggat cgtttcgttg atcctgttcc tcagctcctc cagatgggga agtgcgatct
     4921 ccctgaactc catgtttctt ctcataagac cgctgagctg cagtattctg ttgccgagca
     4981 tgtaattgtt gccgtcccgg gcgaggtagt ccagattcgt catcgcggcg agtatattgt
     5041 gaacggtggc tttgggatag ccgagcctgg cgaccatatc gctgaatcgc catttgtttt
     5101 tctcgaaaaa cagttcgaat atctcatcga cctttttgaa tgccttgaag tgttccgtca
     5161 tattggactc cattcaatat tgtcgtaagt agtaatagga tatcagaaaa attcacgctt
     5221 tgcccggggt gtcgattttt agacactctg actcccgttg aaagcggtcg ctcccgctt
//
LOCUS       DAOOLY010000174         5271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18808_length_5271_cov_5.268021, whole genome shotgun sequence.
ACCESSION   DAOOLY010000174 DAOOLY010000000
VERSION     DAOOLY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18808_length_5271_cov_5.268021"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..696)
                     /locus_tag="PLD15_08720"
     CDS             complement(64..696)
                     /locus_tag="PLD15_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPB64031.1"
                     /translation="MKISRVISHFFPNRCEACDCEIEVDAVLCSECGKLIKGPIMAPF
                     ELAHIDRAYSYWSYDTPLRELIHAYKFGDRPKMAGLFSKMLLEMFLAFGGGVDTVVPV
                     PTTAEAFRKRGFDTNHLILKKLSGDLDVKIEDVLVATGKRSPQSRLKLADRHGNVEGK
                     FRVKRELSTGKIILFDDVVTTGATVGQCARVLREKGVKEITLFSIAKAKK"
     gene            complement(693..3251)
                     /locus_tag="PLD15_08725"
     CDS             complement(693..3251)
                     /locus_tag="PLD15_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPB64032.1"
                     /translation="MEDILLGKVFRRDSRKRISLRYVDQEKPFVSLDIEELDGYMKGL
                     GNGCLFIPGLADAEKLTEYSVDERFLKNFLAAATAEDVRDRIREYSETLKKLPKGLTS
                     ILVRLPGREGLRNFLQKFLKMSQVIEPWEDSISSSLELVERRERKTQRIDIQKAIRLV
                     FSEGGLLQDLLSGYEYRQEQFMASLEIAESIVNENGIMIEAGTGTGKSMAYLAPIAYA
                     CVSTMNQAVVATRTRVLQDQLVKKDLPVIRQLPNLKDLRSLSLKGRERYLCLRKLYES
                     LRIVSNGLVASDSAWEIFGVLLWSILTPDGDLDSLTLGDDTRMTLAGNRFDCTRRLCP
                     LYDTCPYYVARDNARNADIVVTNHSLLFSEANLRIMDGEEEDFEALGTLLPSFRYLVL
                     DEAHEVENSLTQAMSFTLDPAEAVSSLKKAIKSVRSCFGFLKRHFDEEFLRKLWVKMN
                     AATEELEGILRELVEHGRTLSNGEKRTFTKETLPELVEILLKMADRLQIHIAVSGQTI
                     QMLDDVQEQSEGKGEELSMILKGLHAELREWARQIAGITATESSKVVYIHKQERLNGN
                     GLKITSSPVQNDRIMSSVFPNVKVKIFISATLWVYSKGSDGFNYARRVIGAREDYPAI
                     RLGTSFDYTQQLKFFVPSDMPDYRPNSLSYLESASRFIETALSIVDGGSLVLFTSYDD
                     MKTTAGRIEKNLEGITVNVQERNDSPAILLAEHINSKRSVIFGTRAFWEGIDLPGEQL
                     KLLIVYKLPFERPDEPLIEARIKHYGTRNPSEGMNKYYYPKMITAFRQGIGRLIRSRR
                     DHGVVIVLDARMVDPNRPYSKKLRASMTPGIEIQSVRSNQIFTRLRSLKKEGWL"
     gene            complement(3304..3391)
                     /locus_tag="PLD15_08730"
     tRNA            complement(3304..3391)
                     /locus_tag="PLD15_08730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Leu,seq:gag)
     gene            complement(3458..3673)
                     /locus_tag="PLD15_08735"
     CDS             complement(3458..3673)
                     /locus_tag="PLD15_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPB64033.1"
                     /translation="MPRVVRLFQIKNLVKEDEADRIAKKLRSLDGVVHVQVDVNSGVL
                     EIEYENAIIDRYVIREELLKIGFEMLI"
     gene            complement(3677..4609)
                     /gene="fmt"
                     /locus_tag="PLD15_08740"
     CDS             complement(3677..4609)
                     /gene="fmt"
                     /locus_tag="PLD15_08740"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB64034.1"
                     /translation="MRIVFMGTPDFAASHLEALLESNYNVVGVFSQPDRPKGRGRKLT
                     PTPVKLVSLKYGIPVFQPDSVNTGDGFDTLCKLSPDVIMTVAYGKLLKKRVIELPSLG
                     CYNVHASLLPRYRGAAPIQRTLENGETETGITIFKIDEGMDSGPIAVAESIEIEMSDS
                     FGTLFEKLQTLGRKMLLNFAASLDNRNPFLTPQSDEEASFAPKILPDDLLLKDFSSAT
                     RVFNKIRAFDPAPGVRTSLGGEIVKLFGARPGAEETIGIDGEIVEIDDSGMVVSCGSG
                     SVIISLVQFSGKKKMHPVDALSGRLLGKGMVLGG"
     gene            complement(4614..5102)
                     /gene="def"
                     /locus_tag="PLD15_08745"
     CDS             complement(4614..5102)
                     /gene="def"
                     /locus_tag="PLD15_08745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB64035.1"
                     /translation="MKVIYIGNPLLRLVSKKIEVFDEKLREFVEELSKAMYVEDGVGL
                     AAPQVGVSKRIFVYDAGEGIRVVINPEFLWKSEETVQMEEGCLSIPGIYADLFRPSSV
                     KLRYQDVEGNFHEEELSEYAARIVQHEADHLEGVLFVDHLSSAKRALLKSKLNQIMRE
                     KV"
BASE COUNT         1102 a         1474 c         1329 g         1366 t
ORIGIN      
        1 cggactctgg atccgccgat ggaggttcga gtcctcctct cccagccagg ggccttcgag
       61 gccctatttt tttgctttcg cgatcgagaa gagcgtgatt tctttaacgc ctttctctct
      121 taagactcta gcacattgcc caacggttgc accagtcgtt acgacgtcgt cgaagagtat
      181 tattttcccg gtcgaaagct cccttttgac acggaacttg ccctctacgt ttccatgtct
      241 gtcggccagt ttgagacgcg attgtggcga tctcttaccc gtcgccacaa gaacgtcttc
      301 gatcttcaca tccaggtctc cggagagttt tttcagtatg aggtggttgg tgtcgaagcc
      361 cctctttctg aaggcttccg cagttgtagg aacgggtacg accgtatcta cgccccctcc
      421 aaaggccagg aacatttcaa ggagcatttt cgagaagagg ccggccattt tcggcctgtc
      481 gccgaatttg taggcgtgaa tcaattctct gagcggggta tcgtaactcc agtaagagta
      541 ggccctgtct atatgcgcga gctcaaaagg agccatgatc ggccctttta tcagctttcc
      601 gcactcgcta cagagaacgg cgtctacttc gatttcgcag tcgcaggcct cacacctgtt
      661 cgggaaaaag tgcgaaatca ccctcgaaat cttcaaagcc acccttcctt tttcaaactt
      721 ctcagccggg taaaaatctg gttactcctg acgctctgga tctctatgcc cggcgtcatg
      781 gacgcccgga gcttcttcga gtagggtctg ttgggatcga ccattcttgc gtcgagcact
      841 atcaccaccc cgtgatccct tctagatctt atgagtcttc cgatcccctg tctgaaggcc
      901 gttatcatct tcgggtagta gtatttgttc atgccctccg aagggttgcg ggttccgtaa
      961 tgctttatcc tcgcctctat cagaggttca tccggccttt cgaacgggag tttgtagacg
     1021 attagcaact tgagctgctc accggggagg tcgatccctt cccagaaggc cctggtgccg
     1081 aagatgaccg atctcttcga atttatatgc tcggccagca ggatggccgg gctgtcgttt
     1141 ctctcctgaa cgttgactgt tatcccttcc aggttttttt cgatcctccc ggccgttgtc
     1201 ttcatatcgt cgtaagaggt gaacagtacg agagatcccc cgtccactat gctcaaggca
     1261 gtctcgatga accgggaggc gctttccaga taagatagag agtttggcct gtaatcgggc
     1321 atgtccgagg gcacgaagaa tttcaactgc tgggtgtagt cgaaggatgt gccgagcctt
     1381 atggccggat aatcctccct ggcgcctatc acgcgccttg cgtagttgaa tccgtcgctg
     1441 cccttgctgt aaacccacag ggtcgccgag atgaagatct tgaccttcac gtttggaaag
     1501 acgctggaca tgatcctgtc gttctgaacg ggcgaggagg ttatcttcaa tccatttccg
     1561 tttaatctct cctgcttgtg tatatagacg actttcgagc tctccgtcgc cgttatgccg
     1621 gcgatctgtc tggcccactc gcggagctcg gcgtgcaacc ccttcaggat cattgagagc
     1681 tcttcgccct ttccctcgct ctgctcctgg acatcgtcga gcatctgtat cgtctgaccg
     1741 gaaacggcga tatggatctg gagtctgtcg gccatcttga ggagaatttc taccagttcc
     1801 ggaagggtct ctttcgtgaa agtcctcttc tctccattcg aaagcgttct gccgtgctcg
     1861 accagttccc gcagtatgcc ttccagctct tctgtcgccg cgttcatttt gacccagagt
     1921 tttctgagaa actcctcgtc gaagtggcgc tttagaaaac cgaaacagct tctgacggat
     1981 tttatggcct tcttgagtga agagaccgcc tcggccgggt caagcgtgaa actcatggcc
     2041 tgggtcagcg agttttccac ctcgtgagcc tcgtccaaaa ccaggtatct gaaggacggc
     2101 aggagggtgc cgagggcttc gaaatcctcc tcctccccgt ccatgatcct caggttcgcc
     2161 tcgctgaaga gcagagagtg gttggtgacg actatatcgg cgttcctggc gttatcgcgg
     2221 gctacgtaat aagggcaggt atcgtacagc gggcataatc tccttgtaca gtcgaatctg
     2281 ttccctgcca gagtcatcct tgtgtcgtct ccgagcgtga gcgagtcgag atctccgtcg
     2341 ggagtgagga tagaccagag aagaacgccg aagatctccc aggcggaatc gctggctacc
     2401 agtccgtttg aaacgattcg aaggctctcg tacagcttgc gcaggcagag gtatctttcc
     2461 cttcccttga gggagagcga tctgagatct ttgaggttcg gcagttgtct tatgaccggc
     2521 agatctttct ttacgagctg atcctgcagg actctggtcc ttgtcgcgac gacggcctga
     2581 ttcatggtgg atacgcaggc ataggcgatc ggggccaggt aggccatact cttgccggta
     2641 ccggttccgg cctcgatcat gatgccgttt tcattcacta tcgactcggc tatctccaga
     2701 ctcgccatga attgctcctg ccggtattcg tatccggaca gaagatcctg caggagaccg
     2761 ccttcgctga agaccagacg gatggctttc tggatatcta ttctctgggt tttcctctcc
     2821 ctacgctcga ccagctccag agaggatgag atggaatcct cccagggctc gatcacctgg
     2881 ctcatcttca aaaacttctg gaggaagttc cgcagccctt ctctgccggg gagcctgacg
     2941 aggattgagg tcagcccttt ggggagtttt tttagagtct ccgagtactc tcttatacgg
     3001 tctcgaacgt cctccgcggt cgccgcggcg aggaagttct tcaggaacct ctcatctacc
     3061 gaatactctg tgagcttttc ggcatcggcc aaaccgggaa tgaaaagaca gccgtttccc
     3121 agacccttca tgtatccgtc gagctcttcg atatcgagcg agacgaaagg tttttcctgg
     3181 tccacgtatc tcagcgatat cctttttctg gaatctctcc tgaaaacctt accgagcagg
     3241 atatcctcca aactgcacct ctccgaaaag cgaaaagaaa aaccgggcct aaacccggtt
     3301 cggtggtgcc gagggcggga cttgaacccg cataggctaa acgcccacat ggacctcaac
     3361 catgcgtgtc tgccaattcc accacctcgg caacttcacg agaaaaatta taacttgacc
     3421 ggattccttt gtcaagaagc cacaacgcgg ataaagctca tatgagcatc tcgaaaccta
     3481 tcttgagaag ttcttctctg attacgtaac gatcgataat cgcgttctcg tattcgatct
     3541 cgagaacacc gctgttcaca tctacctgca cgtgcacgac tccgtccagc gaacggagct
     3601 tcttcgctat cctgtcggct tcatcctctt taacgaggtt tttgatctga aagagcctga
     3661 cgactctcgg caaagttcat ccccccaaaa ccataccttt tccgagcagt cttccactca
     3721 aagcatcaac gggatgcatc tttttcttgc cggaaaactg cactaacgat atgataacac
     3781 ttcccgaccc gcagcttaca accattccag agtcatctat ctcgacaatc tctccgtcga
     3841 ttccgatcgt ttcctccgct cccggtctgg cgccgaacag tttgacgatt tctcctccca
     3901 gagaggtcct gactccggga gcggggtcga aggccctgat tttgttaaag acacgcgtgg
     3961 cgctggagaa atctttcaac agaagatcgt cgggaagtat cttcggggca aaagaggcct
     4021 cttcgtcgct ctgcggtgtc aggaaagggt tgcggttatc gagagaagcg gcgaagttca
     4081 gaagcatttt cctcccgaga gtctggagct tttcaaagag cgtaccgaaa ctgtcggaca
     4141 tctctatctc tatcgattcg gccacggcga tcgggccgct gtccatgccc tcgtctattt
     4201 tgaaaatcgt gatcccggtc tcggtctcgc cgttttccag cgtccgctga atcggagcgg
     4261 cgcccctgta ccttggaagc agcgacgcgt gaacgttata acatccaagc gaggggagct
     4321 cgatcactct ttttttcaag agctttccgt aggctaccgt cattataacg tcgggcgaga
     4381 gcttgcacag agtatcgaac ccgtctccgg tgttgactga atcgggctgg aagacgggaa
     4441 taccgtattt gagagatacc agcttaaccg gggtgggcgt caacttccgg ccgcggcctt
     4501 tgggtctgtc gggctgtgaa aagactccca ctacgttgta gttgctttcc agcagcgctt
     4561 ccagatggga ggccgcgaaa tccggtgttc ccatgaagac tattctcatg ggactagact
     4621 ttctccctca ttatctgatt cagtttggat tttaaaaggg cccttttggc ggacgagagg
     4681 tgatctacga agagaacgcc ctcgaggtga tcggcctcgt gctggactat gcgcgcggcg
     4741 tactcggaga gttcttcctc gtggaagtta ccctctacgt cctgatagcg gagcttgaca
     4801 ctcgagggtc tgaagagatc ggcgtagatg ccgggaatgc tcaggcagcc ctcctccatc
     4861 tgcactgtct cttcgctctt ccagaggaac tcggggttga taaccactct gatcccttcg
     4921 cccgcgtcat agacgaagat ccttttcgaa acgccgacct gcggagcggc caggcctacg
     4981 ccgtcttcga cgtacatcgc cttgctcagt tcctcgacga actccctcag tttttcgtcg
     5041 aaaacctcga tttttttgga aacgaggcgt aataaggggt tccctatgta aatcactttc
     5101 atctaagcag cgtcaggaga cacagggctt cagcccggtg aggaatgacg cctccctcct
     5161 ttcacataat aatgatgatg atctttctac aagttttagt tttcttacaa caatgcagac
     5221 taacatatag tccgcctcct tacggagtaa ttctcccatc gccaatgtta t
//
LOCUS       DAOOLY010000175         5247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18916_length_5247_cov_4.472072, whole genome shotgun sequence.
ACCESSION   DAOOLY010000175 DAOOLY010000000
VERSION     DAOOLY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_5247_cov_4.472072"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLD15_08750"
     CDS             <1..1406
                     /locus_tag="PLD15_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB64036.1"
                     /translation="RDENSELLDLERVVMKELPGEERIDSFIEFVKGYSREQAIAEAA
                     RCVDCELCVEACPVNMDIPRYIESVFHDDTEEGANWIYKTNPLPSVCGRVCTHKCETV
                     CSIGNRGKPLAIRWLKRYIMDQEKTEDVIRYASNGEVVKKACGKVAIIGAGPAGLSAA
                     YYLSLMGYSATIFESKALAGGVMRYGIPRYRLPDEALDKDIKVIESLGVEIKCLTTVG
                     KDVTLEEIRDEYDAVFLGTGFSKGRSTGVEGVDSEGVIMAIPLLEKIRDYLRQGDGEK
                     PPVTDSVIVIGGGNVAMDAARSVARIQKMERNHIDVKVTSLESMEEMPADLEEILEGK
                     EEGIKYFPSRGPKEVLLKDGKIYGLKTIACTRVFDDDGRFNPRFDESDLSTIEGTMII
                     EAIGQAPDYDFLPVEIKGEIQFIRGRILVNEKGQTALAWLFAGGDIVNGPDIIHGVAD
                     GHRAAIGIDEFLAGVKK"
     gene            1403..1900
                     /locus_tag="PLD15_08755"
     CDS             1403..1900
                     /locus_tag="PLD15_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB64037.1"
                     /translation="MSDLKKILELALSFERSGQEFYKSNMEKVKQVLARETFMYLMEM
                     ESSHVEFIKKMIEKLKTDETINIEATGEKDEIFKERLKSQALSSNTYSNDLADLSILR
                     MAYLIEKDFVEYYGKASEKIEDDRAKKLLTTLRVWEEGHMELVKRLMERIYEKNALDL
                     GFYPF"
     gene            2133..>5247
                     /locus_tag="PLD15_08760"
     CDS             2133..>5247
                     /locus_tag="PLD15_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPB64038.1"
                     /translation="MKRKLLFFASVAALLMLLLTGCPNGPKSFTLTITTSPDTALQIT
                     IDLVNQQTPFSQKYVEGTNVNVQVTSPQERDGSAFVVGNDTKYTFQQWNDSNNSNPRN
                     FTVNGNFTYTAQMNVQYKVDVSSSPAGALVTGGGWYNKNANATLTAPAMAGYIFDHWV
                     VNGVNIGNTNPLTVPVNEPKNVVAHYVSAQFTLTVTTTPDTGLVIRIAGVQYNSPRTV
                     SVSEGAVVQISVDTPQERDGSAFVVGNDTKYTFQQWNDASNTNPRNVTVNGNVTYTAQ
                     MGVQYKVDVSSSPVGALVTGGGWCNKNANATLTAPAMAGHVFSHWVVNGVNVGNTNPL
                     IIPVNEPKNVVAQYNADVQYTLTVTTLPDIALDVRIDGTAYPSPKSMVVQGGSNKQIA
                     VVAYQEKDVSPWMTGIDSKYTFVNWNDGSAQNPRNVTLDSDKTFTANMTTEYRIDVSA
                     SPTLWETGVYWTPRGAVWQFDFSGDLGPYNFSYWLVNGQNVGSARPLELTIDKPHNVT
                     AVFAMQEPDYTLTVTTSPHTNLNISIGGTPYTSPKSVVLNSGTATQINVTSPQSKDTS
                     AQVAGDDTKYTFQQWNDLNTQNPRNITVYSDITYTAQMKLEYLVATSSNPAGTAIAGA
                     GWHIAGEIVNFSAPQKAGFNFSHWVVNGVNAGAANPLAVTIDTPKNVVAHYVAAAGRN
                     IWGNVTPYTGDVKTADLDEYDIISNPDIRTVPEEAEYVEGEYILKVTSFKEAESFFKT
                     ATTGSIEILRRIESADDSLRFLLVSTDLSMKELENLTGVVSASRNYLFYPLVTTPNDT
                     YYPVQWNYPLMNLPQAWDYTVGSRSVVVAIIDSGFSVNHPDLTGVFTAGYNFIDNNTN
                     VSEPNTSEDSHGTHVAGTVAALSNNALGVAGVTWGGFGISIIPIRGIKDSSTLISSLI
                     YAVDHGAKIINMSLGGPAGTEPLHDAIKYADRNGVVMVAASGNNGDGNILYPAKYPET
                     IAVGAVWKDGSTVKRSNYSCFGPELDVVAPGGWMTSYTDPNGIYSTGWTPAGNNYMYM
                     QGTSMATP"
BASE COUNT         1465 a         1303 c         1404 g         1075 t
ORIGIN      
        1 ctcgcgacga aaactctgaa cttctcgatc tggaaagagt tgtgatgaaa gagcttcccg
       61 gagaggaacg catcgactcc tttattgagt tcgtgaaggg ttattcgaga gaacaggcca
      121 tagcggaagc ggccagatgc gttgattgtg aactctgcgt ggaagcctgc cctgttaaca
      181 tggacatccc ccgatacata gagagtgtct tccatgatga cactgaggaa ggagccaact
      241 ggatttacaa aaccaacccg cttccatcag tttgcggaag agtatgtacc cacaaatgcg
      301 aaactgtatg ctcgattggt aatagaggaa agcctcttgc gataaggtgg ctgaaacgtt
      361 acatcatgga ccaggagaaa actgaagacg tgatacgtta tgcttcgaac ggagaggtag
      421 tgaagaaggc ctgcgggaaa gtagccataa taggtgcggg ccctgccgga ctttcggccg
      481 cttactatct ttcgctgatg ggatactctg cgaccatatt cgaaagcaaa gctcttgccg
      541 gaggagttat gaggtacgga ataccgagat atagacttcc tgacgaagcc ctggacaaag
      601 acataaaagt tatagagtcg ctcggggttg aaatcaagtg tctcactacc gtcggaaagg
      661 atgtaacttt agaggaaata cgcgacgaat acgatgcggt tttcctggga actggattct
      721 ctaagggtag gagtaccggt gtcgaagggg tagatagtga aggggtaatc atggcgatac
      781 ctcttctaga aaagattcgc gactacctaa ggcaaggaga tggagagaag ccaccggtaa
      841 ccgatagcgt tatcgttata ggtggaggca acgtcgcgat ggatgcagct agaagcgtag
      901 cccgcataca gaaaatggaa aggaatcaca tcgatgtgaa ggtaacatcg ctagagtcga
      961 tggaagagat gccggccgat ctggaagaga ttctcgaagg taaggaagaa ggcataaagt
     1021 actttccatc tcgtgggcca aaagaagttt tgttgaaaga tggaaagatc tacggattaa
     1081 aaactatagc ttgtacaagg gtctttgacg acgacggtag atttaatcct cggttcgatg
     1141 aatcagatct ctcgactata gaaggtacga tgattattga ggcgattggt caagctccag
     1201 attatgactt tcttcctgtc gaaataaagg gagagattca attcataaga ggcagaattc
     1261 ttgttaacga aaagggacag actgctttag cgtggttatt tgctggagga gacatagtga
     1321 acggaccgga tataattcac ggcgttgccg acggccatag agctgcgatc ggtatcgatg
     1381 aattcttggc gggggtgaag aagtgagtga tctgaaaaag attctggaac ttgcccttag
     1441 ttttgaaaga tcagggcagg agttttacaa aagcaacatg gaaaaagtta agcaagtgct
     1501 ggcaagggaa acctttatgt atctgatgga gatggaatcc tcacatgtgg aatttataaa
     1561 gaaaatgatc gaaaaactca aaaccgatga gaccataaat atcgaagcaa ccggagaaaa
     1621 ggacgaaatc ttcaaagaac gtctgaagtc acaggctctt tccagtaaca cctacagcaa
     1681 cgatttggcc gatctttcga tactgagaat ggcttacctt atagagaaag acttcgtgga
     1741 gtattacggt aaggcctcgg aaaagataga agatgatcgg gccaaaaaac tgctgacaac
     1801 tctcagagtt tgggaagagg gacacatgga gttggtgaaa agacttatgg aacgtatcta
     1861 tgaaaaaaac gcgctggatc tgggcttcta cccgttttaa tcgaaaggtg attatcgatc
     1921 tacaccgatg aatttcggaa acgaacagag ccgatgaatg gaacctgaac gtttaaaaag
     1981 tgatagtatc tttctatacg agcgtaagac catagcagaa ttcaactctg gctatcagca
     2041 ccgtcagtac gctcttgctt tcgtgttcca tatttggcgt tcaatgcagt gaagaatgac
     2101 catagttttt ctattagaga ggaggtcgtg caatgaaaag gaagcttctg ttcttcgcat
     2161 cggtggcggc tctgttgatg ttgcttctta ccgggtgtcc caacggtcca aaaagcttca
     2221 ctttgacaat aaccacttcg cccgatacgg cgctgcagat aactatcgac ttggtcaatc
     2281 aacagacgcc cttcagccag aagtacgtgg agggaacgaa tgtgaacgtc caggtcacaa
     2341 gccctcagga aagggacggt tcggcgttcg tagtcggcaa cgataccaaa tacaccttcc
     2401 agcaatggaa cgattcaaac aacagcaacc cgagaaattt caccgtcaac ggaaatttca
     2461 cgtacacggc ccagatgaac gttcagtaca aagtcgacgt ctcatccagt ccggccggcg
     2521 cgctcgtgac cggcggcggc tggtacaaca aaaacgcgaa cgccacgctg acggcgccgg
     2581 cgatggccgg ctacattttc gaccactggg tagtcaacgg tgtcaacatc ggcaatacga
     2641 atcctttgac tgttccggtg aacgaaccca aaaacgtcgt ggcccattat gtatccgcgc
     2701 aattcaccct gactgttacc accacccccg acacaggcct ggtcataaga atcgccggtg
     2761 tccagtacaa ctcgcccaga acggtttcgg tgagcgaagg cgcggtcgtg cagatctccg
     2821 tagacactcc ccaggagagg gacggttcag cgttcgtagt cggtaacgat accaaataca
     2881 ccttccagca gtggaacgac gcgagcaaca ccaaccccag aaatgtcacg gtcaacggaa
     2941 acgtgactta cacggcacag atgggagtgc agtacaaagt cgacgtctcc tccagccctg
     3001 tcggtgctct cgttacgggc ggcggctggt gcaacaaaaa cgcgaacgcc acgctgacgg
     3061 cgccggcgat ggccggtcat gtcttcagtc actgggtagt caacggtgta aacgtcggaa
     3121 atacgaatcc tctgattatt ccggtgaacg aacccaaaaa cgtcgtcgcg cagtacaacg
     3181 ccgacgtaca gtacaccctg acggtcacca cccttcccga catcgctctg gacgtgagaa
     3241 tagacggaac ggcttatccc tcccccaaga gcatggtggt acagggtgga agcaacaagc
     3301 agatcgccgt ggtcgcctac caggaaaaag atgtgtcgcc gtggatgacg ggaatcgatt
     3361 ctaaatatac atttgtgaac tggaacgacg gcagcgcgca gaatccgaga aacgtcaccc
     3421 tcgattccga taagaccttc accgccaaca tgactaccga gtacaggatc gatgtcagcg
     3481 cctcgcccac actgtgggag acaggcgttt actggacacc cagaggagcc gtctggcagt
     3541 tcgatttctc cggtgacctc ggaccttaca acttcagtta ctggctggtc aacggtcaga
     3601 atgtgggaag cgcgagacct ctggaattga ccattgacaa gcctcataat gtaaccgccg
     3661 tttttgccat gcaggaaccc gattacacgc tgacggtcac cacctcgccc cacacgaatc
     3721 tgaacataag catcggggga actccttaca cctctcccaa gagcgtggtg ctcaacagcg
     3781 ggacagccac acagatcaat gtgacttctc cgcagagcaa ggatacttcc gctcaagtcg
     3841 ccggagacga cacgaaatac accttccagc agtggaacga tctcaacacg cagaacccga
     3901 gaaacatcac cgtgtattcg gatataacct acacggccca gatgaagctg gagtatctgg
     3961 tggcaacttc gtccaacccc gccggaacgg ctatcgcggg cgcgggctgg cacatcgccg
     4021 gagagatcgt gaacttctcg gcgccccaga aggccggttt caatttcagt cactgggtcg
     4081 tgaacggtgt caacgcaggg gccgccaacc cgctggccgt aacgatagat actcccaaaa
     4141 acgtcgtggc ccattacgtc gcggcggccg gcaggaacat ctggggaaac gtgactccgt
     4201 atacgggaga cgtgaagacc gccgatctcg atgagtacga tatcatatcc aaccccgata
     4261 taagaacggt tcccgaagag gccgagtacg tcgagggaga atacatactg aaagtgacct
     4321 ccttcaaaga ggcggaaagc ttcttcaaga ccgcgacgac cggaagtatc gagatcttga
     4381 gaaggatcga atcggccgat gattccctga ggttcctcct tgttagtaca gatctttcaa
     4441 tgaaggagct cgaaaatctg accggcgtgg tgagcgcatc ccggaactac ctcttctatc
     4501 cgctggttac aacacctaac gacacgtact accccgtgca gtggaactac ccgctcatga
     4561 acctgccgca ggcctgggac tacaccgtcg gttccaggag cgtcgtggtt gccataatcg
     4621 attcgggatt cagcgtaaac catcccgacc tgaccggggt attcaccgcc ggatataact
     4681 tcatagacaa caacacgaac gtttctgagc ccaacacctc ggaagacagc cacggcactc
     4741 acgtagccgg aactgtggcg gccctttcga acaacgcgct gggagtggcg ggagtcacct
     4801 ggggaggttt cggcatctcc ataatcccga taaggggaat aaaggactcc agcactctca
     4861 ttagcagtct catatacgcg gttgaccacg gggcgaagat aatcaacatg agtcttggcg
     4921 gaccggccgg gaccgaaccg ctccacgacg ccataaagta cgccgacagg aacggtgtcg
     4981 tcatggtggc ggcctcgggg aacaacgggg acggtaacat actgtacccc gcgaagtacc
     5041 ctgaaacgat agcggtcggc gcggtctgga aagatggaag cacggtcaag agatcgaatt
     5101 actcctgctt cggtcccgaa ctggacgtcg ttgcaccggg aggctggatg acatcctaca
     5161 ccgacccgaa cggaatatac agcaccgggt ggactcctgc gggaaacaac tacatgtaca
     5221 tgcagggcac atcgatggcc acgccac
//
LOCUS       DAOOLY010000176         5233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_18988_length_5233_cov_5.593086, whole genome shotgun sequence.
ACCESSION   DAOOLY010000176 DAOOLY010000000
VERSION     DAOOLY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18988_length_5233_cov_5.593086"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLD15_08765"
     CDS             complement(<1..199)
                     /locus_tag="PLD15_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPB64039.1"
                     /translation="MRRLAILGSTGSIGTQTLDVIEKIEGLEIVAMSCGHNFSLFTKQ
                     LERYRPAFAASLSTEENPLALS"
     gene            complement(202..894)
                     /locus_tag="PLD15_08770"
     CDS             complement(202..894)
                     /locus_tag="PLD15_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPB64040.1"
                     /translation="MTIVYSVLLMAILGVGAGVFLAFASAKFAVKEDPRVKLIEAALP
                     GVNCGACGFPGCPAFAKAMAEGKATLDGCLPGRRSGVPEKLKVIMDSETEKLVALYEE
                     ADEDPQKALAKLLESSGKEIKQAPQKPVRPSQEEIDTYKEKLRDDKRATLIYSVLPNI
                     NCGICGSPGCAAFALKVAAKEEQAEKCVPGKRQSVPDRVAKIMSLKDEEVSKVLEETS
                     GDPAEIKKKFEV"
     gene            complement(905..1486)
                     /gene="rsxA"
                     /locus_tag="PLD15_08775"
     CDS             complement(905..1486)
                     /gene="rsxA"
                     /locus_tag="PLD15_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HPB64041.1"
                     /translation="MATRLLFIFLSAILINNFVLSRFLGICPFLGVSKKIDTAIGMSL
                     AVIFVMILASIITWLLNILLVSLGLSFLTTIVFILIIATLVQFVEIVLKKTSPGLYEA
                     LGIYLPLITTNCAVLGVALLNVQENYTLIETVVNSAGAALGFALALILFSTIRERMEL
                     SDIPAPFKGTASALITAGLLSMAFMGFQGLVKL"
     gene            complement(1499..2110)
                     /locus_tag="PLD15_08780"
     CDS             complement(1499..2110)
                     /locus_tag="PLD15_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPB64042.1"
                     /translation="MAESKFTILTNGFFKQNPIFAQVLSMCPTLATTTSAENGLGMGL
                     ATTAVLAMSNITISLVRKFIPDKIRIPAYIVIIASFVTIIDLLMHGFIYDLWKTLGLF
                     IPLIVVSCIILGRAEAFASKNNVFDSLLDGLGMGLGFTGALVLLGSVREFLGNGSIFG
                     AHLSDVKMFVMILPPGAFIALGMLIALFNYLGIRREKAKKATK"
     gene            complement(2110..2832)
                     /locus_tag="PLD15_08785"
     CDS             complement(2110..2832)
                     /locus_tag="PLD15_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPB64043.1"
                     /translation="MRDYFKTGITLMIITVIAAFALSAVYSIVKEPIANAELGAKLLA
                     IRSVLKDSTSGGSLVSDERIPSTAGDLAKFEWSPSGFQVEDGIIYRSDNGRGKVDNPV
                     YRFTSDDGREIYIAIASAVGYGGDVKTMASFISTGTGLKLNGIKVLEYSQETPGLGAN
                     IANSDIQKRFYPVEPSGLERELKVNKDAGVTPIGEEIDLKRETEGIVTVTDVMTGATI
                     TPRAVTVSINTMYQFLKKAGVK"
     gene            complement(2825..3766)
                     /locus_tag="PLD15_08790"
     CDS             complement(2825..3766)
                     /locus_tag="PLD15_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPB64044.1"
                     /translation="MKLTMTAAPHLRSGNSVRSIMLDVLIALTPAAIWAVVTFGIKAL
                     VLILVCTAGAEALDFFIIRFLRNKKSFRPDLSGSVTGLLLALNLSLAVEWWQALIGVF
                     VSIAIAKAVFGGLGKNFFNPALVGRVFLLISFPVQMTSWFSPFDLQTSATPMAILKEG
                     SSRIFSLQEMFLGTIPGSLGEISALLLLLGFGWLLLRRRVSPVIPVAYIGTVALMAAI
                     FNGVNPAFGSPLYHILGGGLMLGALFMATDMVTSPMSIKGHLIFGVGCGVITMVIRFF
                     GGYPEGVSFAILVMNAFVPLIDMVSKPKIFGKVKSNA"
     gene            complement(3763..5082)
                     /gene="rsxC"
                     /locus_tag="PLD15_08795"
     CDS             complement(3763..5082)
                     /gene="rsxC"
                     /locus_tag="PLD15_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPB64045.1"
                     /translation="MKLPTFRGGVHPPERKELAKDVALRILPLPEKVYVFLANHAGVP
                     AKPVVGPGERVKTGQLIAQSNGFISANLHSPLTGEVKEIGKYYHPTLSKPDDAIVIER
                     TSEDDWELLGPETSFEKLSPGEITERIKQAGIVGLGGAMFPTSVKLSPPKDKKIDLLV
                     INGAECEPYLTIDYRYMLERANGVVRGTLAIMKALGVQRAVIGIEDNKPQAVAKMSEA
                     VKGTGIEVRKLITKYPQGAEKQLIFALEKRVVPSGGLPLDVGVVVDNVGTAYAVYEAV
                     EKGRPLIERGITVTGEGVKSPANFVARIGTMAQELIAFAGGSDEETSKVIFGGPMMGM
                     TVPKIDVPVVKGTSGITLMKDTLPEEEFPCIRCGNCTFVCPMGLQPFLLNLYGSARQY
                     DKAVENGLMDCIECGSCSYICPARIELVKNFKLHKKIYRSLKGGAKK"
BASE COUNT         1241 a         1433 c         1225 g         1334 t
ORIGIN      
        1 aagagagcgc taggggattt tcttcggtgg agagggaagc cgcgaaagcc ggcctatacc
       61 tttccagttg tttggtgaag agagagaaat tgtggccgca actcatggcc acaatttcca
      121 gtccttcgat cttttcaatt acatccagag tctgagttcc aattgagccc gttgagccca
      181 atatggcaag tcttctcaac ttcacacctc gaatttcttc tttatctcgg ccgggtctcc
      241 ggaagtttct tccagtactt tgctgacttc ctcgtccttg agcgacatga tctttgccac
      301 tctgtcggga acgctctgcc tctttcccgg gacgcacttt tctgcctgct cctcctttgc
      361 cgctactttc agtgcgaagg ccgcacaacc cggggaaccg cagattccgc aattgatgtt
      421 tggcaacaca gagtaaatca gtgtagccct tttatcgtcc ctcagtttct ctttgtaggt
      481 gtctatctct tcctgggagg gtctcaccgg tttctgggga gcctgcttta tctctttgcc
      541 cgagctctcc agaagtttag cgagtgcctt ctgcggatcc tcgtccgctt cctcgtaaag
      601 cgcgacgagc ttttcagttt cgctgtccat tatgactttc aacttttcgg ggactcccga
      661 tcttcttccg ggcaaacagc catccagagt tgccttacct tcggccatcg ctttcgcgaa
      721 ggccggacat cccggaaaac cgcacgctcc acaattgacg cctggcagtg ctgcctctat
      781 cagtttaact ctaggatcct ctttgacggc gaattttgcg gaggcgaaag cgaggaacac
      841 accggcgccc actccaagaa tcgccattaa gagtaccgaa taaactatcg tcatagctca
      901 cacctcacag cttcacaagg ccctggaaac ccatgaaggc cattgataga agtcccgccg
      961 ttatcagtgc gctcgccgtt cccttgaagg gggcggggat atctgaaagt tccatcctct
     1021 ctcttatcgt agagaaaaga ataagtgcga gcgcaaatcc aagcgcggca cctgctgagt
     1081 taaccaccgt ttcgatcagg gtgtagttct cctgaacgtt taaaagagca accccgagga
     1141 ctgcgcaatt ggttgtgata agcggcagat agatacccag agcttcgtaa agacccgggc
     1201 tagtcttctt cagcactatc tccacaaact gcacgagcgt cgctattatt agtatgaaga
     1261 cgatggtcgt caaaaaggaa agtcccagag agaccagcag aatattcaac agccatgtga
     1321 ttatcgatgc caggatcatc acgaatataa cggcaaggct cataccgatg gctgtatcga
     1381 tctttttgga aactcctaga aagggacaga tccccaggaa cctagagaga acgaagttgt
     1441 taatcaatat cgcagataag aatatgaaca acagtctggt cgccatactt caacctcctc
     1501 acttagtcgc tttcttcgct ttttcgcgtc ttatcccaag gtaattgaag agggcgataa
     1561 gcattccaag agctatgaag gcaccgggtg gcaggatcat gacgaacatc ttgacgtctg
     1621 acaggtgtgc cccgaagata gaaccgtttc cgaggaactc tctcaccgat ccaagcaaga
     1681 ccagcgcgcc cgtaaaaccc aatcccatgc ccaggccgtc gagaagagaa tcgaagacgt
     1741 tgttcttgga cgcgaaagcc tccgcacgac caagaattat gcagctgacc acgatcagtg
     1801 gaatgaacag tcccagcgtc ttccagagat cgtaaatgaa gccgtgcata agcaaatcga
     1861 ttattgtgac gaacgacgct attataacga tatacgcagg tattctgatc ttgtctggaa
     1921 tgaatttccg caccagcgat attgttatgt tggacatcgc cagtacggct gtagttgcca
     1981 gtcccatgcc caggccgttt tcggcgctgg ttgtggttgc cagcgtcggg cacatgctaa
     2041 gaacttgagc gaaaatcgga ttctgtttga aaaacccatt ggtcagtatc gtaaatttcg
     2101 attcggccat tacttcacac ctgccttctt caggaactga tacatcgtgt tgatcgatac
     2161 ggtgacggcc ctcggagtta tcgtcgcgcc ggtcatcacg tccgtaaccg taacaattcc
     2221 ttcggtctct cttttcagat cgatctcctc gccgattgga gtgacacccg cgtctttgtt
     2281 gactttcagc tctctctcca ggccggaagg ttccaccgga tagaatctct tttgaatatc
     2341 ggaattggct atgttcgctc ccagaccggg cgtctcctgc gaatattcga gaacctttat
     2401 tccattcaac ttcaatccgg tgccggtaga aatgaatgac gccatcgttt tgacatctcc
     2461 gccgtacccc acagcacttg cgatggctat atagatctcc ctcccatcgt cggatgtgaa
     2521 cctgtaaacg ggattgtcta cctttcccct tccgttgtcg cttctgtata ttatcccgtc
     2581 ttcaacttga aaacccgaag gagaccactc gaatttggca agatcgcccg cggttgaagg
     2641 aatcctttca tcgctcacca atgatccgcc actggtagaa tccttcaaaa cgcttcttat
     2701 tgccagtaat ttcgctccaa gttcggcgtt cgcgatgggc tccttgacga tcgaatagac
     2761 cgcagaaagg gcgaaagctg ctattactgt aattatcatc agtgtgattc cagttttgaa
     2821 ataatcacgc attggacttc accttcccga agatctttgg cttggaaacc atatctatca
     2881 ggggtacgaa ggcgttcatg accagaatcg cgaaggagac gccttcggga tatccgccga
     2941 agaatctgat caccattgtg atgactccac atcccactcc gaatatcaag tgtcccttga
     3001 ttgacatagg gcttgtcacc atatcggtag ccatgaagag cgctccaagc ataagtccgc
     3061 ctccaagtat gtgatacagt ggcgatccga aagccggatt gactccgttg aagatcgcgg
     3121 ccataagggc cacagtacct atgtaagcaa ccggtatcac cggagagact cttctgcgaa
     3181 gcaacagcca gccaaatccc agtagcagca gaagagcgct gatttcgccc agagaaccgg
     3241 gaatggttcc gaggaacatc tcctgtagtg aaaagatcct tgacgaaccc tctttgagga
     3301 tcgccattgg cgtcgcgctg gtctggagat cgaaggggct gaaccagctt gtcatctgaa
     3361 cagggaagga tatcaaaaga aagaccctgc ccactagagc ggggttgaag aagttcttcc
     3421 caagtcctcc gaatactgcc ttggcgatcg ctatggatac gaagactccg atgagggcct
     3481 gccaccactc cacagccaga gagaggttca gggccagaag gagaccggtt acggagccgc
     3541 tcaggtccgg tctgaaactc tttttgtttc tcaagaagcg gataatgaag aaatcgagtg
     3601 cttcggcccc tgcggtacag accagaatca gcaccagggc cttgattccg aaagtaacta
     3661 ctgcccagat tgcagcggga gtgagcgcta tcaggacatc cagcattatg cttcttacac
     3721 tgttgcccga cctcaggtga ggcgcggccg tcattgtcaa cttcactttt ttgcccctcc
     3781 cttcagactg cggtatatct ttttgtgcaa cttgaagttc ttcaccagtt cgattctcgc
     3841 cggacagatg taagaacagc tcccacattc tatacagtcc atcaagccgt tttcgacggc
     3901 tttgtcgtac tgtcttgccg atccgtaaag gtttagaaga aacggctgaa gccccatggg
     3961 gcagacgaac gtacagttgc cgcaccttat gcagggaaac tcttcttcgg gcagggtatc
     4021 tttcatcaaa gttattcccg atgtgccttt tacaacaggg acatctatct ttggaacggt
     4081 cattcccatc atcggaccgc cgaagattac tttcgaagtc tcttcgtcgc tccctccggc
     4141 gaaagctatc aattcctgag ccatggttcc gattctggcc acgaagttcg cgggtgattt
     4201 tacgccttcg cccgtgacgg tgattcctct ctcgataaga ggtctgccct tttcgaccgc
     4261 ttcgtaaacc gcgtaggccg ttcccacgtt atcgacgacc acgccaacgt ccagcggaag
     4321 accgcccgaa gggaccactc tcttctcaag cgcgaatatc aactgctttt ccgcgccttg
     4381 cggatatttg gttatcagtt ttctgacttc gatcccagtt cctttcacgg cttcgctcat
     4441 cttcgctaca gcctgcggtt tattatcctc tattcctata acggctctct gaacccccag
     4501 agccttcatg attgccagtg ttccccttac gacaccattg gcgcgttcca gcatgtaccg
     4561 gtagtctata gtgaggtaag gctcgcactc ggcgccgttg atcacaagca gatcgatctt
     4621 cttatctttt ggaggcgaaa gctttacact ggttggaaac atggctccac cgagaccgac
     4681 gattccggcc tgctttattc tctctgttat ctctccgggc gagagcttct cgaaagaagt
     4741 ttcgggcccc aggagttccc aatcatcttc cgaggtcctc tcgatgacga ttgcatcatc
     4801 gggtttagac agggtgggat ggtagtattt tccaatttcc tttacctcgc ccgtgagtgg
     4861 agaatggaga ttagcggaga tgaaaccgtt cgactgggct atcaactgac cggtcttaac
     4921 tctctctccc ggaccgacga cgggctttgc cggaacacct gcatgatttg caagaaaaac
     4981 gtagactttt tctggaagcg gaaggattct caacgcaaca tctttagcca gttccttcct
     5041 ctctggcgga tgcacgcccc ctctgaaggt tggcaacttc atcgtatcac ctctcaacgg
     5101 ttttggcaat gactgactta gttttcgata atatcccgca aaaaaacatc tttgcagctc
     5161 cctgaagctg cagtcacgat tacccgtaaa caattcttac aaacaaaagg agcggatcgc
     5221 tccgctccta tta
//
LOCUS       DAOOLY010000177         5228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_19007_length_5228_cov_5.531026, whole genome shotgun sequence.
ACCESSION   DAOOLY010000177 DAOOLY010000000
VERSION     DAOOLY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19007_length_5228_cov_5.531026"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLD15_08800"
     CDS             <1..699
                     /locus_tag="PLD15_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPB64046.1"
                     /translation="YTPPGAAVVIRNNLPEAIAVVGKGSHIFRDDLDAIVELAKKADS
                     IVIGPGIGRDEETVETVLELIKIFSKSRMVLDADGLYALSKDLTVLSHTEDILLTPHP
                     GEFRRLGGGETGLKSLMDFCEKFRCRVILKGSSSVLCNPDGKLSINIAGTTGLAKGGS
                     GDLLSGTIGSFAAQTGDLERAAILGMYFMGKAAELSDESDASNTASSIGKEYSRVFSC
                     LVGRVRGEKHGIGT"
     gene            683..1879
                     /locus_tag="PLD15_08805"
     CDS             683..1879
                     /locus_tag="PLD15_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HPB64047.1"
                     /translation="MELGHEVSQKLEQRQGLSLRSQQALKLLQLNSQEITAELEEIVQ
                     ANPLLEIKRDDYDLIEDSEDKERRNEDEENVEDGDLYSQPIQYSVYMREESSDREGWD
                     FDRLEAQQPTFREILKDFAYYVLDDRQYGLFETLLDNMDDYGLLSRDLEAVSKEGNCS
                     LEELDSIIGVLRESGFDGVFARDREELSELMDGGLYPSSGYSDGTYVRYVEPDLYIEF
                     FDEKFMVTVRDCSLILNIEDSYREILENGEKSARDYLEDKLEEAQFFVSALERRKTTL
                     LAIGNEIVMRNAVFLLNRGDKIRPLKMNEIADKLGVVVSTVSRAVKGKFIQTPAGTFP
                     LRYFFGNLQEKEQALEIIAEIVRNNEEISDSRLVNELNNRGISIARRTVNKYRNLLGL
                     GKDSQS"
     gene            1876..2526
                     /locus_tag="PLD15_08810"
     CDS             1876..2526
                     /locus_tag="PLD15_08810"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPB64048.1"
                     /translation="MKAGLFLGGQYPSSNDYYSRRISKMGVIVAADSGAEKLRELERP
                     PEILVGDMDSISPATLDWCKKSGAQILIFPPEKDDTDTTLAIKTLYERGALDVDIFGA
                     SGRREDHFLATLFSIYGADTGMKLLITEENFQAGLMKDGDSCTIMEAIEGETWSFLPF
                     GGGLPVVTLEGFKYPLKGQTLDYTRPLGVSNVATGPLVKVLCRGGALIYFRWLKEF"
     gene            2508..3044
                     /gene="yfcE"
                     /locus_tag="PLD15_08815"
     CDS             2508..3044
                     /gene="yfcE"
                     /locus_tag="PLD15_08815"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HPB64049.1"
                     /translation="MVKRILILSDTHGAFEITKAIIDKFGPFEMILHCGDYLYHGPRN
                     PIPADYDPISLSTLFKDMGDRLVGVKGNCDSEIDLTLIGKDRLPDSRVVHINGLDIYM
                     YHGHRKESFPFERGLVISGHTHLRRLVREGSLLLLNPGSPSLPKDASPGSFGTIDFKK
                     REISVFNLTGARSEGETF"
     gene            3114..3740
                     /locus_tag="PLD15_08820"
     CDS             3114..3740
                     /locus_tag="PLD15_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB64050.1"
                     /translation="MLLKWHGHSCFSVVSKERTLLIDPFDEGVGYPLPAVRPSIIVES
                     HQHHDHNSHDRYAGEYVLINRTGKTQVHGFSIEGHPTFHDEFGGKKRGPNIVFEITTH
                     DGFRIVHCGDLGHRLTEELSSRLEGPDVLLIPVGGVYTIDAAVAREITKRIGPSFVVP
                     MHYKTKALRFELGSVDSFIAGEPFESLKELRLEAKSADGTKIVVLSYS"
     gene            3754..4080
                     /locus_tag="PLD15_08825"
     CDS             3754..4080
                     /locus_tag="PLD15_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPB64051.1"
                     /translation="MDEKKLMSLLGIGRKANKVVFGKDQLRSYLRQPVKNKILFLASD
                     TSASIKEDWISRCKSHGARCVLLKEHDRIALGKAIGKNNISAVAISDGGLAEEISKMM
                     TQAGGD"
     gene            4085..>5228
                     /locus_tag="PLD15_08830"
     CDS             4085..>5228
                     /locus_tag="PLD15_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HPB64052.1"
                     /translation="MPKARVYELAKRLNISARELLDELEELGVTVKNHMSVLDDETVN
                     IIVGLYEEEKKETARKNEKAKIKVQEKEVKKLEKKLVSKEETPLEKKAPVQQAARTIK
                     LKSDEFKLDTLAKKMNIPISKIIKDQFMKGIILRPGQILSMDDTRNIASQYEWEIELV
                     QEDEVNPFERLVESYQNTYREGVRLVQRPPVVTVMGHVDHGKTTLLDRIRKAKVAEGE
                     IGGITQSIGAYQVEIRGKKITFIDTPGHEAFTEMRARGAQATDIVVLVVAADDGVMPQ
                     TIEAYNHAKTANVPIIVAINKIDKPNANIDLTKQQLASKLGLVPEDWGGDTVTVPVSA
                     KTGKGVEDVLEMILLVAEVADIKCIPQGHARGIIIDSRLDKAVGPLA"
BASE COUNT         1446 a         1191 c         1458 g         1133 t
ORIGIN      
        1 tacactcccc cgggagcggc cgtggttata cgtaacaacc ttcccgaagc aattgcggta
       61 gtcggcaagg ggtcgcacat atttcgcgac gatctcgatg cgatagtcga actcgcgaaa
      121 aaggccgatt ctatcgttat tggtccggga atcggaagag atgaagagac ggtagaaacc
      181 gttctggagt tgatcaagat cttctcaaaa agccggatgg tcctggatgc cgatggtctg
      241 tatgctctct cgaaggattt gacggtacta tcgcacacgg aggacatcct cctgaccccc
      301 caccccggcg agttcaggcg tctgggcgga ggcgaaaccg gtctaaaatc tctgatggat
      361 ttctgcgaga aattccggtg tagagttatc ttgaagggat cgagttccgt tttgtgcaac
      421 cccgacggaa agttgagcat aaacatcgcc ggcacgaccg gactcgcgaa gggcggcagc
      481 ggcgatcttt tgagcggtac gattggaagt tttgcggctc agaccggaga tctcgaaaga
      541 gccgccattc ttgggatgta tttcatgggg aaagcggcgg aattgagcga tgaatctgac
      601 gcgtcgaata ccgcttccag tatcgggaaa gaatactccc gggtattttc ctgtctggtt
      661 ggaagagtaa ggggagagaa acatggaatt gggacatgaa gtatctcaga agctcgaaca
      721 gaggcaaggc ttatccttaa gatctcagca ggccctgaag ttgctgcagt tgaactctca
      781 ggaaataacc gcggagctgg aggagatagt gcaggccaat cctctgctcg agatcaagag
      841 ggacgattac gatctcatcg aagacagtga ggataaagaa agaagaaacg aagacgaaga
      901 gaacgttgag gatggagatc tctactctca gccgatacag tattcggttt acatgagaga
      961 agagagttca gacagagaag gctgggactt cgacaggctc gaggcgcaac aacctacttt
     1021 cagagagata ctcaaggatt ttgcttacta cgttctcgac gatcgacagt acggtctttt
     1081 cgaaacgctc ctggataaca tggacgacta cggtctcctc agcagggatc tcgaagcggt
     1141 atcaaaggag ggtaactgca gtctggagga actcgactcg attatagggg tactcaggga
     1201 gagcggtttc gatggtgtct tcgccaggga cagggaggaa ttgagcgaac tgatggacgg
     1261 ggggctttac ccctcctcgg gctacagcga cggaacttat gtccggtatg tcgagcccga
     1321 tctgtacatc gaatttttcg acgagaaatt catggtgacc gtcagggact gttcactgat
     1381 tctcaacatc gaggattctt acagagagat ccttgaaaac ggtgagaaga gcgccaggga
     1441 ttatttagaa gataagcttg aagaggccca gttctttgtc agcgctctcg agaggagaaa
     1501 gacaacgctt ctggccatag gaaacgaaat agtgatgaga aacgctgtct ttctcctcaa
     1561 ccggggagat aagataagac cgctcaagat gaacgaaatt gccgacaaac tcggcgttgt
     1621 ggtgtcaacc gtaagccgcg ccgtgaaggg taaattcatc cagacacccg caggtacttt
     1681 tcctttgagg tatttctttg gaaacctcca ggagaaggaa caggctctgg agatcatagc
     1741 ggagatcgta agaaataacg aagagatctc agattcccgg ctggttaacg agttgaacaa
     1801 cagggggata tcgatcgcca gacgcaccgt gaacaagtac cgtaaccttc tgggcctcgg
     1861 aaaggacagc cagtcttgaa ggccggactc tttctgggag gtcagtaccc ttcctcgaat
     1921 gattattact cgagaaggat ttcgaagatg ggtgtgatag tcgccgccga ttctggcgcc
     1981 gaaaaactcc gcgaactcga gagaccgccg gagatactgg tgggagacat ggattcgata
     2041 tctcccgcga ctctcgactg gtgcaaaaaa agtggcgccc agatcctgat ctttccgccg
     2101 gagaaggacg atacggacac gactctggcc ataaagaccc tttacgaaag gggtgcgctc
     2161 gatgtcgata tcttcggtgc atcgggaaga cgtgaggatc acttcctggc gacgcttttc
     2221 tctatctacg gcgccgatac cggcatgaaa ctcctcataa ccgaggagaa cttccaggcc
     2281 ggtctcatga aggatggtga ttcctgcacg atcatggaag ctatcgaggg cgaaacctgg
     2341 tcttttctac ctttcggtgg tggccttccg gtcgtgactc tggagggatt caagtatcct
     2401 ctaaaagggc agactctcga ttacacaagg ccgctcgggg tttcaaacgt ggccacggga
     2461 cctctggtta aagtgctgtg cagaggaggt gcgcttatct atttcagatg gttaaaagaa
     2521 ttctgatact gtccgataca cacggggctt ttgaaataac aaaagcgatc atcgataaat
     2581 tcggaccttt cgaaatgatc ctccactgcg gagattatct ctaccacggt ccgcgcaatc
     2641 caattcccgc cgattacgac cctatttccc tttcgacgct cttcaaagat atgggggatc
     2701 gactggtggg tgttaagggg aattgcgata gcgaaataga tctgactttg atcggtaaag
     2761 acagacttcc agactcgaga gtcgtacata taaacgggct cgacatttac atgtaccacg
     2821 ggcacagaaa ggagagtttt cccttcgaaa ggggattagt gatcagcggc catacccatt
     2881 tgcggagact ggtgagagaa ggctccctgc ttttgctcaa tccgggaagc ccttcacttc
     2941 caaaggacgc ctctcccggt tctttcggta caattgactt caaaaaaaga gagatctccg
     3001 ttttcaatct caccggagcc aggtcggaag gcgaaacttt ctgactctct gcgacggctt
     3061 tagaaaaaca tgttacagaa gccatgatat aataagtatc tgggtgaggt attatgttgt
     3121 tgaaatggca tggacattca tgtttttctg tggtttccaa agagaggact ctactgatag
     3181 accccttcga tgagggagtc ggttatccgt tgcctgcggt aaggccgtcc ataatagttg
     3241 aatctcatca gcaccacgac cacaattctc acgacagata tgcaggcgag tacgttctga
     3301 ttaacagaac cgggaagacg caggtccatg gattctctat cgaaggtcat cccaccttcc
     3361 acgatgaatt cggcggtaag aagcgcggtc ccaacatcgt gtttgagata accactcatg
     3421 acggtttcag gatagtccat tgtggcgatc tcggtcacag actcaccgag gaactgtcga
     3481 gcagactcga ggggccggat gtcttactga tcccggttgg tggtgtctat acgatcgatg
     3541 cggccgtcgc cagagagata acaaagagga tcggtccctc gttcgtggtc cccatgcact
     3601 acaagacgaa agccctgagg ttcgaactgg gttctgtaga ttcgttcatc gccggcgaac
     3661 ctttcgagag cttgaaggag ctacggctgg aagctaagag tgcagatggg acgaagatag
     3721 ttgttctgag ttacagctga tcgaaggtga agcatggacg aaaagaagtt gatgtctctc
     3781 ctgggaatcg gtagaaaagc aaacaaggtt gtgtttggca aggatcaact gagaagttat
     3841 ttgaggcagc cggttaaaaa caaaatactt tttctggcga gcgataccag tgcttccata
     3901 aaagaagact ggatttcaag atgcaaaagc cacggtgcca ggtgcgtcct cctcaaagaa
     3961 cacgaccgta tcgctctggg aaaagccatc ggaaagaaca acatatcggc ggtagcgata
     4021 tccgacggtg gtttagcaga ggagatttca aagatgatga cgcaggcggg aggtgactga
     4081 gcttatgcca aaggccagag tgtacgagct tgctaaaaga ttaaatatat cggcaagaga
     4141 actgctggat gagctggaag agctcggtgt tactgtcaag aaccatatgt cggttctcga
     4201 cgatgaaact gtgaatatca tagtgggcct ctatgaagaa gagaaaaaag aaacggcgag
     4261 aaagaacgaa aaggccaaga ttaaggttca ggaaaaggaa gtcaaaaagc tcgaaaagaa
     4321 gctcgtttcc aaggaggaga cgcccctgga gaaaaaggct cctgtccagc aggcggccag
     4381 aacgataaaa ctcaaaagcg atgaattcaa actcgatacg ctggccaaga agatgaatat
     4441 tccaatttcg aagataatca aagaccagtt catgaaaggc ataatcctga gaccgggaca
     4501 gatactttcc atggacgaca cgagaaacat cgcctctcag tacgaatggg aaatagaact
     4561 cgttcaggaa gacgaagtta atcccttcga gaggctcgta gagtcctacc agaacaccta
     4621 ccgggagggg gtcaggctgg ttcagagacc gccagtggtc actgtgatgg gtcacgttga
     4681 ccacggaaag acaacgttgc tcgacaggat aaggaaggct aaggtcgccg aaggtgagat
     4741 cgggggcata acacagtcca taggtgcgta ccaggtagag ataaggggta agaagataac
     4801 cttcatcgat acgcccggcc acgaagcctt cacggagatg agggcgcgcg gtgcacaggc
     4861 tacggatatc gtggttctgg tagtggcagc ggacgacggc gttatgccgc agaccataga
     4921 ggcttacaat cacgcgaaaa ccgctaacgt accaataatc gtcgctataa ataagataga
     4981 caaacccaac gccaacatcg acctcacgaa gcagcaactg gcttcgaagc tgggcctggt
     5041 tccggaagac tggggaggcg acaccgttac cgttcccgta tctgccaaaa ccggcaaagg
     5101 agtcgaagac gtcctcgaga tgattctact ggtcgccgag gtggccgata taaagtgcat
     5161 acctcagggt catgcgcgcg ggataatcat cgattccagg ctagacaaag ctgtggggcc
     5221 tctggcca
//
LOCUS       DAOOLY010000178         5193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_19178_length_5193_cov_5.255547, whole genome shotgun sequence.
ACCESSION   DAOOLY010000178 DAOOLY010000000
VERSION     DAOOLY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19178_length_5193_cov_5.255547"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLD15_08835"
     CDS             <1..313
                     /locus_tag="PLD15_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64053.1"
                     /translation="YIRTARAKGVPENIVIQKHAKKNAMIPVLTVAGGSVIGLLGGTV
                     FVETIFSRVGMGRFLADAATLLDYWSIIGGALFFSFIMIVGNLVVDVSYALVDPRIRL
                     E"
     gene            327..1208
                     /locus_tag="PLD15_08840"
     CDS             327..1208
                     /locus_tag="PLD15_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64054.1"
                     /translation="MAVKRKSEFRKIMKKYWKNGTAVLGTCLLVFFIIVAVFAPQIAG
                     VNEMGNNYQIPRVSWSSKPIAPTSEHPFGVIGGRDIFYGVVWGTRTAFRLGLIITGFS
                     ALIGVAIGSISAYFGGWVDEILMRIVDIFLSIPFLVAAMVMTTILGKGLDKVIIALII
                     FGWMSTARLIRGNILQAREEQYVLAAKALGQKDWKIIMKHILPNTIFPVVVQMSMRIG
                     SYVITAAGLSFLGVGAEPGYADWGTILSYSRNWMTMLNESWFAIVFPGTAMVLFVLAW
                     NLVGDALRDIFDPRMRS"
     gene            1222..2238
                     /locus_tag="PLD15_08845"
     CDS             1222..2238
                     /locus_tag="PLD15_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64055.1"
                     /translation="MDSKNALLQVKDLRTYFHTEDGIVKAVDGVTFDVYSGETLGIVG
                     ESGCGKSVTSLSVMRLLDEKGEIAGGKIIFEGRDVMAIPESQMMKIRGNDMAMIFQEP
                     MTALNPVFTVGFQIMEAILLHQDVNKEQARTMAIDMLRKVGIPEPEKRVDEYPHELSG
                     GMRQRAMIAMALSCNPKLLFADEPTTALDVTIQAQILELMKSLQEQYGMALVMITHDL
                     GVIAEMAQRVVVMYAGKVVEYAEVLTLFKKPRHPYTWGLMNAIPKLDEDKEVLYNIPG
                     VVPDPLDFPDGCRFNTRCPLATDKCRKEEPPLVEVEENHTSACWYVDKLVEMIKVSRA
                     GEGA"
     gene            2235..3428
                     /locus_tag="PLD15_08850"
     CDS             2235..3428
                     /locus_tag="PLD15_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64056.1"
                     /translation="MSKITDSRLLLRADRLVKHFPVRAGVFRRVVAQVKAVDDISFDV
                     YEKETLGLVGESGCGKTTAGMSVLRLYEPTSGRIIMEGEDTTYYFMPALKAQKYIRDI
                     YIDRFTELKSKGGSAEAAIQSFQNDFDRKIAERYFKEFNEDRNAFVRSMLSNREEKRK
                     AFRRNAQIIFQDPFSSLNPRMRVRNIIGEGARIHGLATGSDVLDKVADIMTKVGLSKD
                     HMSRFPHEFSGGQRQRIGVARALILNPKLIVCDEAVSALDVSIQAQILNLLNDLQKEF
                     GLTYLFIAHDLGVVKHVSRRVAVMYLGKVAELADKKKLFDDPLHPYTVSLMSAIPEAN
                     PEKKKQRIILQGDVPSPINPPSGCRFHPRCPIAKEICAREEPQLQDVGGGHFVSCFFP
                     GQLKR"
     gene            complement(3455..>5193)
                     /gene="priA"
                     /locus_tag="PLD15_08855"
     CDS             complement(3455..>5193)
                     /gene="priA"
                     /locus_tag="PLD15_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPB64057.1"
                     /translation="IVDYLLSVSSEEISVLEKKLGLNSRSPIETLVRKGILSMEEVEE
                     DDSQWVIPSVVDLTQEQKKVSRALLDKRNGVSLLYGLTGTGKTEVYFSVMEYWLNRAK
                     QVLLMVPEVSLTPQLLARVRGAFPARVIRQYHSYMTAGQRQRIWLDATEGNVDILVGT
                     RSSLWVPMKRTGLIIIDEEHDASFYQQSVPFYDGVEVAMKKGRLLDIPIILGSATPRV
                     THFYAASTGGMDLLELRNRPVGSFPVIEIIDMKEEKNPIISARALKEIENTLSQGKQV
                     FVFVHRKGFSNYVVCHTCGQTVTCPHCSVSLTYHKIDNAMKCHYCGYRQHVPRSCPTC
                     GSMTLSARGFGTERVENDLQKFFPSVSIMRMDRETVDNPLAYEKALQAISQKKSQIIV
                     GTKMITKGLDFPDVEMVLVIDADRLMSFPSYDSPETAFQHIAQVSGRSGRAGKGRAFI
                     QSFNPGNRVISKAFERDYDSFFRQELDLRKELRYPPFVKIVEIVCTGNSEEESLDLGK
                     QVVAGLEKSKDAYLELFGPIVPLLSKVKSSYRTKIVLKLENDSPLDFLQPLIKKHSAG
                     LQTIVNGIGGML"
BASE COUNT         1292 a         1304 c         1370 g         1227 t
ORIGIN      
        1 ttacataaga accgcgaggg caaagggtgt accggagaat atcgtaatcc agaagcatgc
       61 caaaaagaac gctatgattc ccgttctcac agttgccggc ggttctgtca taggattgct
      121 ggggggaacc gtcttcgttg aaaccatctt cagcagggtc ggcatgggga ggtttctggc
      181 cgatgcggcg acccttctgg actactggtc gataataggc ggggcgcttt tcttctcctt
      241 cattatgata gttggcaatc tggttgtgga tgtatcgtat gctctcgttg acccgcgaat
      301 aaggcttgag tgaggaggca aaatagatgg cagtcaaaag aaaaagcgaa tttcgaaaga
      361 taatgaagaa gtactggaag aacgggaccg ctgttctggg tacctgtctc ttggtcttct
      421 tcataatcgt cgcggtcttt gctccccaga tagccggagt caacgagatg ggaaacaact
      481 atcagatccc gagggtttct tggtcttcca agcccatcgc accaacttcc gaacacccgt
      541 tcggagtaat cggcggaaga gacatcttct acggcgtggt atggggaaca agaacggcct
      601 tcaggcttgg tttaatcata accggtttct cggcattgat cggtgtcgcg atcggatcca
      661 tctccgcgta tttcggaggg tgggtcgacg aaatactgat gagaatcgtg gatattttcc
      721 tttcgatacc tttccttgtc gcggcgatgg tgatgacaac cattcttgga aaagggctgg
      781 acaaagtgat aatcgccctc atcatattcg gctggatgag caccgccagg ttgattagag
      841 gaaacatact ccaggcgagg gaggagcagt acgtcctggc cgcgaaagct ctgggacaga
      901 aggactggaa gattatcatg aagcatattc ttccgaacac tatcttcccg gttgtagtac
      961 agatgtctat gagaatagga tcctatgtaa taaccgcggc gggattgagc ttcctcggag
     1021 taggcgccga acctggatac gcagattggg gaactatact ctcttactcg cgcaactgga
     1081 tgaccatgct caacgagtcc tggttcgcaa tagttttccc cggcacagca atggtccttt
     1141 tcgttctcgc ctggaacctc gtgggtgatg ccctcagaga catcttcgac ccgagaatga
     1201 gaagctgagg aggattttga cgtggatagc aaaaacgcac ttttacaggt gaaagatcta
     1261 agaacctatt ttcatacaga agacggtata gtgaaagcgg tagacggcgt cactttcgat
     1321 gtttattccg gcgaaacgct cgggatagtc ggtgaatccg gttgcggaaa gagcgtcact
     1381 tccctttcgg tgatgagact tctggatgaa aagggcgaaa tcgccggcgg aaagatcatc
     1441 ttcgaaggta gagacgtgat ggccattccc gagtcacaga tgatgaagat cagaggaaac
     1501 gacatggcca tgatcttcca ggaacccatg acggctctca acccggtctt tacggttggt
     1561 tttcaaataa tggaggccat tctccttcac caggatgtca acaaggaaca ggctagaacg
     1621 atggcgatag atatgcttcg caaggtcggt attcccgaac cggaaaagag ggttgacgag
     1681 taccctcacg aactctccgg aggaatgaga cagagagcca tgatagccat ggcgctttcc
     1741 tgcaatccga aactcctctt cgcggatgag cccaccacgg ctctggatgt gaccattcag
     1801 gcgcagattc tcgagctcat gaagtcgctt caagaacaat acggcatggc tctggtgatg
     1861 attacgcacg accttggagt gatagcggag atggcccaga gagtcgtagt gatgtacgcc
     1921 ggaaaggtag tggaatacgc cgaagtccta acgctcttca aaaagccaag gcatccttac
     1981 acctggggtc taatgaacgc gatccccaaa ctcgatgagg acaaagaggt tctctacaac
     2041 atacccggtg tcgttcccga ccctctagat tttccggatg gttgcagatt caacaccaga
     2101 tgtccgctgg cgaccgacaa atgcagaaaa gaagagccgc ccctggtgga agtagaagag
     2161 aaccacacat cggcctgctg gtacgtggat aaactcgtcg aaatgatcaa agtatctagg
     2221 gcaggtgagg gcgcatgagt aaaataaccg atagtagact acttctaagg gccgaccggc
     2281 tggtgaaaca cttcccggta agggccggcg ttttcagaag ggtagtggca caggtcaaag
     2341 ccgtcgacga tatcagtttc gatgtttacg agaaagagac tctcggactc gtcggagaat
     2401 ccggttgcgg taaaaccacg gctggtatgt cggttttgag gctttatgag ccgacttccg
     2461 gtagaataat catggaaggg gaagatacga cctactattt catgcccgct ctgaaagccc
     2521 agaagtatat aagagacata tacatcgacc gattcaccga gttgaaaagc aagggtggat
     2581 cggccgaggc tgcgattcaa tcgtttcaaa acgatttcga tagaaagata gccgaaagat
     2641 acttcaaaga attcaacgaa gaccggaacg ccttcgtgag aagtatgctt tcgaaccgcg
     2701 aagagaagag aaaggccttc agaagaaacg cgcagatcat cttccaggac cccttttctt
     2761 cgctcaatcc cagaatgcgg gtcaggaaca tcatcgggga gggtgccagg atccacgggc
     2821 tggcaactgg aagcgacgtt ctggacaaag tcgccgatat tatgacgaaa gtcggtctct
     2881 ccaaggatca catgtccaga ttcccgcacg agttcagcgg gggacagaga cagcgtatag
     2941 gggtcgccag agccttgatt ctaaatccca aactcatagt ctgcgacgaa gccgtctcgg
     3001 cccttgacgt ctcgattcag gcccagattc tcaatttgct gaacgacctt cagaaggaat
     3061 tcggccttac ctatctcttc atcgctcacg atcttggcgt tgtgaaacac gtcagcagga
     3121 gggtggccgt catgtacctc ggaaaggtag cggaacttgc cgacaagaaa aaactattcg
     3181 acgatcctct acacccttac accgtatcgc tgatgtcggc cattcccgag gccaacccgg
     3241 agaagaaaaa gcaaaggatc atcctccagg gcgatgtccc cagtccgatc aatcctcctt
     3301 ccggctgcag gttccatcca cgctgtccca tagccaaaga aatctgtgcc agagaggaac
     3361 cccagttgca ggatgtcgga ggtggacatt tcgtctcctg cttcttcccc ggacagctga
     3421 aaaggtagaa gaccggtacg atagaataga tctatcaaag cattccgccg attccgttca
     3481 ctattgtctg aagtccggcg gaatgttttt ttatcagagg ttgaagaaaa tcaagcggac
     3541 tgtcgttttc cagtttcagg acgatctttg ttctgtaaga actcttgacc ttcgagagaa
     3601 gcggaactat ggggccgaag agttccagat aagcatcttt cgacttttcc agcccggcca
     3661 ccacttgttt accaagatca aggctttctt cctctgaatt tccggtgcat acgatctcca
     3721 cgatcttgac gaaaggcggg tagcgtaact ctttccttag atccagctcc tgtcggaaga
     3781 aactgtcgta atccctctcg aaggccttgc ttataaccct gttgccggga ttgaaactct
     3841 gaatgaaggc ccttcccttg ccggccctgc ccgagcggcc gctaacctgc gcgatgtgct
     3901 gaaaggcagt ttcaggactg tcgtagctgg gaaaactcat aaggcggtcg gcatcgatca
     3961 ccagcaccat ttcgacgtcg ggaaagtcga gccctttcgt gatcatttta gtccccacta
     4021 ttatctggct ctttttttga gagatggcct gcagagcctt ttcgtaggcg agcggattat
     4081 cgacggtttc gcgatccatg cgcattatgc tgacggacgg aaagaacttc tggagatcgt
     4141 tttccactct ctccgtaccg aacccccgcg ccgaaagtgt catcgatcca caggtcggac
     4201 aggatctcgg cacgtgctgc ctgtatccgc agtagtgaca cttcatggcg ttgtcgatct
     4261 tgtggtaagt cagagagacg gaacaatgag gacaggttac ggtttgaccg cacgtgtggc
     4321 agaccacgta gttagagaat ccctttctgt gaacgaagac gaagacctgt ttgccctgcg
     4381 agagggtgtt ttctatctct ttgagcgccc ttgcgcttat gatagggttt ttctcctctt
     4441 tcatatcgat aatctcgatc accgggaagc ttccaaccgg cctgtttcgg agctccagca
     4501 gatccatgcc gccagtgctc gcagcgtaga agtgtgtaac tctgggcgtc gccgagccga
     4561 gaataatcgg gatatcgagc aaacgccctt tcttcatggc cacctctaca ccgtcgtaaa
     4621 aaggcacgct ctgctggtaa aagctggcgt catgttcttc gtcgattatt atcaggccgg
     4681 ttcttttcat gggaacccac agcgaactcc ttgtcccgac caggatgtcc acgtttccct
     4741 cggtggcgtc gagccagatt ctctgcctct gtccggcggt catgtaactg tggtactgcc
     4801 tgatgactct agccgggaaa gcccccctga cacgcgccag aagctgggga gtcagggaaa
     4861 cttccggaac cattaataga acctgttttg ccctgttcag ccagtactcc atgacgctga
     4921 agtacacctc ggtctttccc gtacctgtaa ggccgtaaag aagcgatacc ccgtttcttt
     4981 tatcgagcag tgctctggag actttcttct gctcttgagt gagatcgacg accgacggga
     5041 taacccattg agaatcgtct tcctctacct cctccatcga gagaattccc ttcctgacga
     5101 gcgtctcgat cggactgcga ctgttcaatc cgagtttttt ctcgagaacg gagatctcct
     5161 cggagctgac ggagaggaga taatcgacta tcg
//
LOCUS       DAOOLY010000179         5164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_19333_length_5164_cov_4.779017, whole genome shotgun sequence.
ACCESSION   DAOOLY010000179 DAOOLY010000000
VERSION     DAOOLY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19333_length_5164_cov_4.779017"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1678
                     /locus_tag="PLD15_08860"
     CDS             <1..>1678
                     /locus_tag="PLD15_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB64058.1"
                     /translation="PEKISPENFRALLSGKLSDRSGEEKYLSRLEREVSVICLKGIQD
                     YFMTVSRIVSLAREIGCWIGPGRGSAAGSLVSYLLSITKVDPVKEGLYFERFLSVKRD
                     DPPDIDIDVEDRMRQELLLKLRDFFGDDRFCLIRTTSTFGFKGAAREIGRELGIDETR
                     VSRLIDWSNDGKRFPRSFERDGEMRRLFDLSRELVGFYSGFSIHAAGVLLSDVPLNTM
                     IPLEREGEFQVSLWDMESLQAMGLQKIDILGLRNLTLLKRLTQGKEPWEYGTSNRKTY
                     EVLKKGYTTGIFQLESPFATRIVKSMDLSSINDVSIAIALNRPGPIKSGVADRYMKLN
                     RVPGELERVRSRDSILSETNGFLVYQEQLLSIAGKELALSLDDGELLRRALSKKDAEK
                     VDQILNRAAGFNSLPDEKRRRVYSFLMNFAGYAFNKSHSLCYALISYWLAYFKANRPG
                     RFYGEVIREVSGHNLSRLVAELRDSGIEISTRGEPARENVELSLSIPSSLGYGELPAR
                     PEEESFFSFVRTHRRKLQAKDLERLIKSGYFDLYGNRKELLRQINNALTG"
     assembly_gap    1679..1778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1779..2341
                     /locus_tag="PLD15_08865"
     CDS             <1779..2341
                     /locus_tag="PLD15_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB64059.1"
                     /translation="YGNRKELLRQINNALTGMAPELKSIRSVFGFKEEVQEKKEYDTD
                     MERALMEIEALGFNLTFIKGPEISDEIADTSVTAALARLQTGLATFRRVDYMGRSYIT
                     DGRTFIEVNDSLPIHGYVVFKDGKPSVWKESVREVSRIVHGSVDRDFIVEASPGESVI
                     IKVKGKTRLIQGARLRTLEPDEILLK"
     gene            2357..2980
                     /locus_tag="PLD15_08870"
     CDS             2357..2980
                     /locus_tag="PLD15_08870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPB64060.1"
                     /translation="MQILFVRHGTTEWNKEKRWQGSSDVPLSDVGRAQARVTARYLLE
                     QFEVPEAIYTSDLSRALETSGFIAGEFGKEPIATPLLREAHIGLWSGVEIEESFKTHG
                     RLIQKWRSDPWADMPETESLGELQRRSVGFLKYVEGLYKDGQSIIVVSHALFIKSVLC
                     YVLGMPLQNNYSFNLDNCSISTIRAGEGGTGWRLFELNIHRHLAQVR"
     gene            2986..3681
                     /locus_tag="PLD15_08875"
     CDS             2986..3681
                     /locus_tag="PLD15_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPB64061.1"
                     /translation="MKIKVNFLPVELSGEVDLAVVVDVLRATSTIVTALHAGASRVMP
                     VETVEEAFATKRREPRVLLAGERGGHMIPRFDLGNSPRDFTEELVRGREIVMTTTNGT
                     PAALRSKGAGKIVAASFLNLARVIEEIGNEPGLVAIQCAGSGGQFSMEDVLLAGAAAS
                     VFEDRLLDDASRAAVFLFRSANVPLDRFLVDHCEHAKKLSSIGYFEDVAFCAKINMYD
                     LLPVWRENGFVRG"
     gene            3685..3897
                     /locus_tag="PLD15_08880"
     CDS             3685..3897
                     /locus_tag="PLD15_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64062.1"
                     /translation="MDQISNVLFIIAMGAFFLSVIFFEIGTKKVRKPKGELKPQDYRL
                     YDKKGWISLLVAGSFLGLSLLFAVVF"
     gene            3918..4556
                     /locus_tag="PLD15_08885"
     CDS             3918..4556
                     /locus_tag="PLD15_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64063.1"
                     /translation="METKLAVEEIVSKLPGVHGARIVNDEDGEMVEIHVLADVSKPAK
                     QLVRDIETAIFAASGVKIDRKIVSIAQIALDSEEKKTSATDSFTKEDSQGYSFQLQSL
                     ETKATSKKLDITITLDHDGKELQGTSIVDLGDDEKYMAVVEATVAAIRDEIPGFRVEF
                     IEKLMYGMNEIVMAVGSSLVNGKRKKEAGARLCKKDSLNDFAMVVLEIVNKI"
     gene            4598..>5164
                     /locus_tag="PLD15_08890"
     CDS             4598..>5164
                     /locus_tag="PLD15_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB64064.1"
                     /translation="MEFSRLVSSVTPSATLEFNKKALELAKAGENVVKFTAGEPDFPT
                     PRPIIDAAILALNEGKTKYTNATGIDELRKRIALKLKADNGLDYSADEIVVANGGKQA
                     IYNVLKAILNPGDEIVIISPAWVSYEAQIRLCGGEPVIVESKIENGFVPEIAEIEKAL
                     TSKTKGIIINSPNNPTGAVYSQKSISDLV"
BASE COUNT         1283 a         1250 c         1483 g         1048 t
ORIGIN      
        1 tccccgagaa gatttcgccg gagaatttca gagcgcttct ctccggcaaa ctttccgaca
       61 ggagcggcga ggagaagtat ctatctcggc tggagagaga agtatcggtc atctgtctca
      121 aggggatcca ggattacttc atgaccgtgt cgagaatagt gtcgctggca cgggagatcg
      181 gttgctggat aggtccgggg aggggctcgg ccgccggttc cctggtgagt tatcttttga
      241 gcataactaa agtcgacccc gtgaaggaag gcctctattt cgaaaggttt ttgagcgtaa
      301 aaagagacga tccgcccgac atagacatag acgtcgaaga ccgcatgaga caggagcttc
      361 tcctgaaact gagggacttc ttcggcgacg atcgcttctg tttgataagg accacctcga
      421 ctttcggctt caaaggcgcg gcccgcgaga tcggcagaga gctggggata gatgaaacca
      481 gggtttcgcg cctcatagac tggagcaacg acgggaagag gttcccccgc tctttcgaga
      541 gagacgggga gatgagaagg ctcttcgatt tatcgcgcga gctggtgggc ttttattcgg
      601 gtttttcgat ccatgccgcc ggcgtgctcc tgagcgacgt tccgctgaat acgatgattc
      661 ccctcgagag ggagggggag ttccaggtct cgctctggga tatggagtcg cttcaggcga
      721 tggggttgca gaagatagat atactgggac tgcgcaacct gacgctcctg aagagactca
      781 cacagggcaa ggagccctgg gagtacggca cgtcgaacag aaagacatac gaggtgttga
      841 agaaaggcta cacgacgggc atttttcaac ttgaaagccc cttcgccacc aggatagtca
      901 aatccatgga cctctcctcg ataaacgatg tttccatagc catcgccctc aaccgccccg
      961 gtccgataaa atccggcgtg gccgatagat acatgaaatt gaacagggtt cccggcgagc
     1021 tggagagagt gaggagcagg gactcgatac tctcggaaac gaacggcttt ctggtctatc
     1081 aggagcagtt gctgagcata gccggcaagg aactggccct gagcctcgac gacggcgaac
     1141 ttctgagaag ggctctctcg aaaaaggacg ccgaaaaggt cgatcagata ttgaaccggg
     1201 ccgccgggtt caacagcctc ccggacgaga aaagaagaag ggtttactcc ttcctgatga
     1261 acttcgccgg ttacgccttc aacaagtcgc acagtctctg ctacgccctg atctcttact
     1321 ggctggccta cttcaaggcc aaccgtcccg gccgcttcta cggagaggtc atcagagagg
     1381 tctccggcca caacctttcc aggctcgtgg ccgagttgcg cgacagcggg atagagatct
     1441 cgacacgggg agagcccgct cgagagaacg tggagttatc cctttccata ccatcttcgc
     1501 tgggatacgg cgagttgccg gcgaggcccg aagaggagag cttcttctct ttcgtcagga
     1561 cacacagaag aaagctgcag gccaaggatc tcgagcggct gataaagagc gggtatttcg
     1621 atctttacgg aaaccgcaaa gagctcctcc gacagataaa caacgcgctg acgggtatnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tacggaaacc gcaaagagct
     1801 cctccgacag ataaacaacg cgctgacggg tatggccccg gagttgaagt ctatcagatc
     1861 ggtcttcggc ttcaaagaag aagtgcagga aaagaaggag tacgacaccg atatggagag
     1921 ggcgctgatg gagatagaag cgctggggtt caacttgacc ttcatcaaag ggcctgaaat
     1981 ttcagatgag attgccgaca caagcgtgac ggcagccctg gcgagactgc aaacggggct
     2041 ggcgactttc agaagggtag attatatggg taggagctat ataaccgatg ggaggacctt
     2101 catagaggtc aacgattcgc tcccgattca tggatacgta gttttcaagg acggcaaacc
     2161 ttcggtgtgg aaggaaagcg tcagggaagt ctcgaggatc gtgcacggga gtgtcgacag
     2221 agacttcata gtagaggcgt cgcccgggga gagcgtaatt atcaaggtga aaggaaagac
     2281 ccgcctcatc cagggcgcac ggctcagaac gctggaaccc gacgaaatac ttttgaagtg
     2341 aaccgggagg aaattgatgc agatcctttt cgtaagacac ggcaccaccg aatggaacaa
     2401 agagaaaaga tggcagggga gttccgacgt gccgctttcc gatgtcggtc gcgcccaggc
     2461 cagggtcacg gccaggtatc tcctcgaaca gttcgaagtt ccggaggcga tctacaccag
     2521 cgatctgtcg agggcgctgg agacttcggg gtttatcgcc ggagagttcg gcaaagagcc
     2581 catcgccaca ccgttactga gagaggccca catcggtctc tggagcggtg tcgaaatcga
     2641 ggagtctttc aagactcacg gcaggttgat tcagaagtgg cgcagcgacc cctgggcaga
     2701 tatgccagaa acggaatctc tgggcgaatt gcagaggcgc tcggtcggtt ttctgaaata
     2761 cgtcgagggg ctctataaag acggccagtc cataatagtc gtctcgcacg cccttttcat
     2821 aaagagcgta ctctgttacg ttctgggcat gccgcttcag aacaactaca gtttcaatct
     2881 cgacaactgc tcgatatcga ccatacgcgc cggtgaaggc ggaacgggct ggaggctctt
     2941 cgaactcaac atccaccgcc atcttgcgca agtgcggtga tctgaatgaa gataaaggtt
     3001 aacttccttc ccgtcgaact ctccggagag gtggacctgg ccgtcgtcgt cgatgtcctg
     3061 agggcgacga gcaccatcgt caccgcactc catgcgggag cgagccgggt gatgccggtg
     3121 gagacggttg aagaggcctt cgcaacgaag cggagagagc cccgggttct tctggccggc
     3181 gaaaggggcg gtcacatgat accaagattc gatctgggaa actcgccccg ggacttcacg
     3241 gaggaactgg tgaggggcag ggagatagtc atgacgacga ccaacggaac tccggccgcc
     3301 ctgcgttcga agggggccgg aaagatagtg gccgcctcct ttctcaacct cgccagggtt
     3361 atcgaagaga tcggaaatga accgggactg gttgcgattc aatgcgccgg gtcgggaggc
     3421 caattctcta tggaggatgt gctcctggca ggcgccgccg cttcggtttt cgaggatcgt
     3481 ctcctggacg acgcttccag agcggccgtg tttctcttca ggagcgcaaa cgttcctctg
     3541 gacagattcc ttgtagacca ctgcgaacac gctaaaaaac tctcctccat cggttatttc
     3601 gaagatgtgg cattttgtgc taaaatcaac atgtacgatt tgctacccgt ctggcgggag
     3661 aatggattcg tgcgggggta gtccatggat cagatatcta acgttctttt cataattgcg
     3721 atgggtgcct tttttctgtc ggttattttt ttcgagatcg gcacgaagaa ggtgcgtaaa
     3781 ccaaagggtg aattaaagcc tcaggattac aggctgtacg acaagaaagg ctggatttcc
     3841 ctcctggttg ccggcagttt tctgggtctt tcgttgctct ttgcggtagt tttttgaagg
     3901 tctagggggt tgattttatg gaaaccaagc tcgcggttga ggaaatcgtt tcaaagttac
     3961 cgggtgtgca cggcgcgcgc atcgtcaacg atgaagacgg tgaaatggtc gaaatccatg
     4021 tgctggcaga tgtctccaaa cctgcgaagc agctggttcg agacatcgaa acggccatct
     4081 ttgcagcctc tggagtcaag atcgacagga aaatagtgtc catagcgcag atagcgctgg
     4141 actccgaaga gaaaaagact tccgcaacgg acagcttcac caaagaagat tcgcagggat
     4201 actccttcca gttacaaagt ctagaaacga aggccaccag caagaaactc gatatcacaa
     4261 tcactctcga ccacgacgga aaagagcttc agggaacctc gatcgtcgat ttaggcgacg
     4321 acgaaaagta catggcagtg gttgaagcga cggtggctgc catcagggac gaaataccgg
     4381 gcttcagagt ggagttcata gagaagttga tgtacgggat gaacgaaatc gtcatggccg
     4441 taggttcttc cctggtgaac ggcaagagaa agaaggaagc cggtgcaagg ctctgtaaga
     4501 aagatagcct gaacgatttc gcaatggttg tacttgagat cgtaaataaa atctgatctt
     4561 ccaagcgtat acaagtattt attgtggagg tgctccaatg gagttttcac gcttagtttc
     4621 ttcagttacg ccttctgcta ctctggaatt caacaaaaag gcactggaac tggcaaaagc
     4681 gggcgagaac gtggtcaagt tcaccgccgg cgagccggat ttcccgacgc ccagaccgat
     4741 aatcgacgcc gccatactcg ctttgaacga aggaaagacc aagtacacga acgcgacggg
     4801 tatcgacgag ttgagaaaga gaatcgccct gaaactgaag gccgacaacg gactcgacta
     4861 ctcggccgat gagatcgtag tcgccaacgg cgggaagcag gctatctaca acgttcttaa
     4921 ggccattttg aaccccggcg acgagatagt tataatctcg ccggcctggg tcagttatga
     4981 agcccagata agactctgcg gcggagagcc cgtgatcgtg gagtcgaaaa tagagaacgg
     5041 cttcgtaccg gaaatcgccg aaatagagaa ggcgctcact tcaaaaacca aaggtataat
     5101 cataaattca ccgaacaacc cgacgggtgc cgtgtattcg cagaagagca tctccgacct
     5161 cgtc
//
LOCUS       DAOOLY010000180         5089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_19753_length_5089_cov_4.615614, whole genome shotgun sequence.
ACCESSION   DAOOLY010000180 DAOOLY010000000
VERSION     DAOOLY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19753_length_5089_cov_4.615614"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..956
                     /locus_tag="PLD15_08895"
     CDS             390..956
                     /locus_tag="PLD15_08895"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64065.1"
                     /translation="MRKVLIVIFLLYLLTMLTSCLIKPVVLTLSAIPQGGGTVAGTGV
                     YLKGDYITITADATECFMFIGWYDVENDSLVSLDREYAFAIQKSQNLAAKFEKAFLEV
                     DFLLLGSNIAGCKYDVKIKVNGRVTKIDFQDRDEISFPKSEVFPDETGWIRFSEFYTN
                     PGVEETTILAYAGNTRVSYCTFSIEQKP"
     gene            1083..4880
                     /locus_tag="PLD15_08900"
     CDS             1083..4880
                     /locus_tag="PLD15_08900"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64066.1"
                     /translation="MKKRALTIAFFVFAVFVLFLLSGCLKFRGEVKIDARFTQVAKWP
                     LLNQNEFKAELSVAKSNAAVSLEIIADGFVSQSDFKLEKGQATVEDLVVESGVKELTL
                     VARDTNGKELARKSVSIAAAEDEFVTGSVDSRTGIYITASSFPVDEDFFLVYPYAGWF
                     GPADIGGERLFVFYSADYLEELSPVDRYRISRVILRAEDPVNAGEAAFLLRVEDIWRY
                     FDFPEAAKKTDYEVAIGNDLISPTAVDARSRRRFDIKDRVYLYEVAFHAVPEGWGDPA
                     INAGSVVYHGSSCAEGVSDNLVADAFPGVDYVFLAWYDDPGLEEAHRIGEADPLLYHL
                     TAGDVPQFPGKFDIYANYGPSYFEITVDVDPSNSGTITVDNHEVTAPYAGDHRYGEEI
                     RLEAFPTDCYDFVGWYENGTEVSISNPYAFEVYHGRDLTARFAISQHTVKIDPDPQDA
                     GKIKVDGAAAVETYEATHLCGSTLEAEAVPGECHYFRNWTEDGQELSTERIYEILVNG
                     DRTIEANFLGWSDLFDSLVTVTVYHGLGMASYDIILRGDASELPPEVVELEIWPNTGV
                     LFVGGYGEEEGPFSQGTPYALTGAKLDATDWPHEITLTYGEGETLDDYPEKLYVRVND
                     TQVCVVELGVPQPAWEGWGSSHSLFTEASPEEGGIVEGAGTYEAGQKVHLTALPAENF
                     EFVNWTATAGSVESPNEAETNYTMPSEDATVTANFARTGYAISFKEVNNLAGVSIQLY
                     KYFSDPFYDYLPVGDPLVTDANGETSIALESGSYKFKASKQGYEDSELYFDVNNKNLD
                     FQIAMQAKRYAVNFKTTAKVLTGLFEIKIYSKASMEEGDLVATLNHGNAETGTANLLA
                     GSYWFRLKHRSTYTGRNVDFVFAGAFEVKDVTDLEYYLENSLALPDDIYKYRIHFTYD
                     HFSTAAAISSEGRFKVEEGLEIDMQMKTDTYEEPGTYLFRNKGQTEVFIEAWGGGGGG
                     GGRSSGRTTTSARGGGGGAYARSVVTITNPAYNIVVGDGGNGGVGNQNGNDGSSSVFG
                     TDLVVAAGGKGGSYIPTGSAGSYAGDGGSTDDSVGDVKYGGGDGDKGGGGGAGSTEDG
                     ENSNGNASGAGGYEGGGAGGWMPVYNGSNNGQKAGGGGSGAYRTSNGGTVNGGKGADG
                     KVRITYIEIPSAVLMKINYIDYDEVYRELPGIGWEHQLIVRMSVQDYDGYPVPDAYVT
                     ATIYRNNSYYRDLLGYVGLSTDFYISPPLEAGFYKTTVQSLEAAGYLWDGVTPNNGKS
                     FSF"
BASE COUNT         1319 a         1173 c         1433 g         1164 t
ORIGIN      
        1 gtacgaggtt gtgaaaggag gaaccctcat gttgtatgca ggagtcgaca tatcgctttc
       61 tcgtgagcgg agcgaactct cgacgttctt tttcggctct taccagcaac aaacaacgca
      121 ccaccaacaa ccgttcttgt taggaacact actggatgac agtcttattc ccttcaggtc
      181 gttttcttgc attgtcaggt ttagaagagg ctttgtattt tgcaagtgaa atgatataat
      241 atctcatcta aaatatacag tcgttggttt gaatacttga aattaccgag taccagggat
      301 gtctttccaa tcgcacattt tcgtaacttg gagtatagcc ggattggaat tctccgcttc
      361 tgaaagatcg tcgagtggag ggaatctgga tgcgaaaagt actgattgtg atcttcttgc
      421 tgtatctcct tactatgctg acttcctgcc ttataaagcc ggttgtgttg acgttgtctg
      481 cgatccctca gggcggcgga accgtagctg ggacgggagt atatctgaag ggtgactata
      541 taacaataac cgcagacgcg accgagtgct tcatgtttat cggttggtat gacgtagaga
      601 acgactctct ggtcagctta gaccgtgaat atgccttcgc catacagaaa agccaaaatc
      661 tggcggcgaa atttgaaaag gcctttctag aagtcgattt tcttttgttg ggttcgaata
      721 tagccggctg caagtacgat gtgaaaatca aagttaatgg aagagtgaca aaaatcgact
      781 tccaagacag agacgaaatc tccttcccaa agagcgaggt ctttccagac gagacgggct
      841 ggatacgatt ctccgagttc tacaccaatc ccggcgtaga ggaaacgacg attctggcat
      901 atgcggggaa cacaagggtc tcttattgca cttttagcat agagcaaaaa ccttagattt
      961 tcttggcact tcttttttgc gcctttgaga agacttttgg atgaaaggat tgttttggat
     1021 tgtcgagtag atttggatcg ccgagaccat aaacggtttt tggacgggga ggtcggtatg
     1081 ccatgaagaa aagagcgctc actatagctt tcttcgtatt tgctgttttt gtgttgtttt
     1141 tgttatccgg ctgtctgaag ttccggggag aggttaagat agatgccagg ttcactcagg
     1201 tcgcaaaatg gccgctgttg aatcagaatg aattcaaggc agagctgtct gtcgcgaaga
     1261 gcaacgccgc ggtaagcctg gagatcattg cggacggatt cgtatctcaa agcgatttta
     1321 agcttgaaaa gggacaggca actgtcgagg atctagtagt cgagagtggc gtcaaagagc
     1381 tgacgctcgt tgccagagat acgaacggca aagagctcgc cagaaaatcg gtctctatag
     1441 cagccgctga agatgaattc gtaacgggtt ctgtcgacag ccgcacgggt atctatataa
     1501 cggcgagctc gttcccggta gatgaagatt tcttcctcgt ctatccttac gcgggatggt
     1561 tcggcccggc ggatatcggc ggagaaaggc tctttgtatt ctactctgcc gattacctcg
     1621 aagagctatc acccgtagac agatacagga taagcagggt gatactcagg gcggaagatc
     1681 cggtgaatgc cggagaggcg gcctttcttc taagggtgga ggatatctgg aggtatttcg
     1741 actttcctga agccgccaaa aagacggact atgaagtcgc gatcggaaat gatctcataa
     1801 gcccgaccgc ggtcgatgct cgctctcgca gaagattcga catcaaggac cgcgtctatt
     1861 tatacgaagt tgcttttcac gctgttcccg agggctgggg agatcccgcg atcaacgccg
     1921 gctcggtcgt atatcatgga agctcctgcg cggaaggagt gtccgacaac cttgtcgcag
     1981 acgcctttcc cggcgttgat tacgtctttc tggcctggta cgacgatccg ggcctggaag
     2041 aggctcacag gatcggagag gccgatccgc tgctctatca tctgacggcc ggcgatgtcc
     2101 cgcaattccc cgggaagttc gacatctacg ccaattacgg gccgtcttat ttcgagataa
     2161 ctgtcgatgt cgatccctct aattccggga cgattaccgt ggacaatcat gaagtgaccg
     2221 caccttatgc cggagaccac cgctatggag aagaaatcag attggaggcc ttccccacag
     2281 attgctacga ttttgtcggc tggtatgaaa acgggacgga ggtcagtata agcaaccctt
     2341 acgctttcga agtctatcat gggagagacc tgaccgccag gttcgcgata agccagcaca
     2401 ctgtcaagat cgatcccgac cctcaagacg ccggaaagat aaaggttgac ggcgcggcgg
     2461 ccgtagaaac atacgaagcg acgcaccttt gtgggagcac actggaggcc gaagcggtgc
     2521 cgggagagtg tcattacttt cgcaactgga ccgaggacgg ccaggagttg agcacggaaa
     2581 ggatatatga gatcctggtg aatggagaca gaactatcga ggcgaacttt cttggctgga
     2641 gcgacctgtt cgattcgctg gttaccgtca ctgtctatca cggattgggc atggcctctt
     2701 atgacatcat tctgagaggc gacgcatcgg aactgccgcc ggaggtcgta gaactcgaaa
     2761 tctggccgaa cacgggagtc ttgttcgtcg ggggctatgg agaagaagaa ggccctttct
     2821 cgcagggaac accgtacgcg ctgaccggtg cgaagctcga tgctaccgac tggccgcatg
     2881 agatcaccct cacttacggc gaaggcgaga cgctggacga ctatcctgaa aagctgtatg
     2941 taagggtcaa cgatacgcag gtgtgcgtcg tggagctcgg cgttcctcaa ccggcctggg
     3001 agggctgggg ttcaagtcat tctctgttta cagaggccag tcccgaagag ggcggaatag
     3061 tcgagggagc cggaacttat gaagcgggtc agaaggttca cctgacggct ctgcctgccg
     3121 agaacttcga attcgtcaac tggaccgcga cggccggctc tgttgaatct ccaaacgaag
     3181 cggaaaccaa ctacacgatg ccctctgaag atgcgacagt taccgcgaac ttcgcacgca
     3241 caggttatgc aatctccttc aaggaagtaa acaatcttgc aggtgtatcg atacagcttt
     3301 ataagtattt ttccgatcca ttttacgatt acctcccggt gggtgatccg ctggtcacgg
     3361 acgcgaacgg cgaaacgtcc atcgcccttg agagtggatc ctacaagttc aaagcttcaa
     3421 aacagggata cgaggactcc gaattgtatt ttgacgtgaa caataaaaac cttgacttcc
     3481 aaattgccat gcaggcaaag aggtacgcgg tgaacttcaa gactaccgcg aaggttctca
     3541 caggactgtt cgagatcaag atctactcga aggcgagtat ggaagaagga gatctcgtgg
     3601 cgactctgaa ccacggcaac gctgagacgg gaacggcgaa ccttctagcg ggaagttatt
     3661 ggttcaggct caaacaccgg tcaacctata ccggaagaaa cgttgacttt gtctttgcag
     3721 gcgccttcga agtgaaagat gtcacagatc tcgagtacta tctcgagaac tcactcgccc
     3781 taccggacga tatatacaag tatagaattc acttcactta cgatcacttc agtactgccg
     3841 cggccatatc ttccgagggc agattcaaag tagaagaggg gctcgagata gatatgcaga
     3901 tgaagacaga tacatacgaa gaacctggga catatctctt ccgcaacaaa gggcagacag
     3961 aggtttttat cgaggcctgg ggtggtggag gaggcggagg aggcagatcg tccggaagga
     4021 cgacgacttc ggctagaggt gggggcggtg gagcctacgc cagaagcgtt gtgaccatta
     4081 caaatccggc ctataacatc gtagtcggtg acggtggaaa cggaggcgtg ggaaaccaga
     4141 acggaaacga cggctcgtca agcgtttttg gaactgatct cgtcgtcgcg gccggcggaa
     4201 agggaggctc ctatattcct acaggcagtg caggctctta cgcgggtgac ggaggatcga
     4261 cagacgactc tgtcggcgac gtgaagtatg ggggcggaga tggagataaa ggtggaggcg
     4321 gaggagctgg ctcaactgag gacggagaaa actctaacgg caacgccagt ggtgcaggag
     4381 gttacgaagg cggaggcgcc ggaggctgga tgcctgtcta caacggttca aacaacggac
     4441 agaaggccgg cggaggagga agcggcgctt atagaacaag taatggagga actgtaaatg
     4501 gaggtaaagg tgccgacgga aaagttagaa taacctacat agaaatcccc tcggcggtcc
     4561 tgatgaagat aaactacata gactatgacg aagtgtacag agagttaccc ggtataggtt
     4621 gggagcatca gctaattgtg agaatgagtg tccaggacta cgatggctat cctgtacccg
     4681 atgcatacgt aacggcaact atctaccgta ataacagcta ttacagagat ctgctcggat
     4741 atgtcgggct ctcaaccgat ttctatattt ctcccccact tgaagcggga ttctacaaaa
     4801 caaccgttca aagtctcgaa gcggccggat acctttggga cggcgtcaca ccgaacaacg
     4861 gaaagagttt cagtttctag attattgaaa ttacggagat gacggatcgg attccgccat
     4921 gacggtatgc gagctgtacg ctggataaga gccgggaaag agcaccgaga gttcgctccg
     4981 ctcacgagaa agcgagagcg aaaataggga ctgacgagtt cctgacatct gtcccatttt
     5041 tcgtgtatag ttctcccatg cagtcatcac gtaatgttcc tatcatgag
//
LOCUS       DAOOLY010000181         5065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_19865_length_5065_cov_5.041317, whole genome shotgun sequence.
ACCESSION   DAOOLY010000181 DAOOLY010000000
VERSION     DAOOLY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19865_length_5065_cov_5.041317"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLD15_08905"
     CDS             <1..488
                     /locus_tag="PLD15_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64067.1"
                     /translation="MDAMMAFPGILFALMFVSIFGVGIRNTIIAIGIMSIPSFARITR
                     SGFIQHKEFEYVKNARVKGVSNFRIMFSHILPNVLSPIIVAASMGFSSAVLSEAALSY
                     LGLGVQPPDPSWGRMLNESQVFITLSPWYTIAPGIFITLIVLGFNFLGDGIADLQDRQ
                     Q"
     gene            497..1483
                     /locus_tag="PLD15_08910"
     CDS             497..1483
                     /locus_tag="PLD15_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64068.1"
                     /translation="MERIISDRALLQVRDLNISFTTAKGDFPAVRGISFDVGRNSIVG
                     LVGESGCGKSVTALAIMRLLPGNARLSGTIDFSGRNIVAMHEEEIRKLRGSEISMIFQ
                     EPLTSLNPLWKVGRQIDENLKMHTELKGDDRFQSVVDIMRDVGLPDPERLYHKYPHEL
                     SGGMRQRIAIAIAVACRPALIIADEPTTALDVTIQAQILQLLKDINREKGSSIIFISH
                     NFGIIREICTSICVMYAGHLVEIAPTEKLFCEPRHPYTIGLMKAIPKTDSKGKELYDI
                     PGKVPSITDTLPGCPFEPRCEYAFERCKRELPQFEVLGEQHESRCFLAGELR"
     gene            1480..2442
                     /locus_tag="PLD15_08915"
     CDS             1480..2442
                     /locus_tag="PLD15_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64069.1"
                     /translation="MSGIILEVRDLVKHYPLRRTKLLESPGRVRALNGVSFAIARGES
                     FGLVGESGCGKTTAGHTIIKLLEPTDGKIFFEGKDITTIKGERLKDLRRKIQIIFQDT
                     AGSLNPRKTVKWILSEPLRAQGVRNRLEIERTIRESLELVDLDESFLDRYPHELSGGQ
                     KQRIGILGALILEPEFIIADEPVSALDVSIQAQILNLMKNLQKRLSLTYLFISHDLGV
                     VHFFCDRIAVMYLGEIVEMAEATELYVNPCHPYTQSLLSAIPGENAASQRIILQGDAP
                     DPTNPPQGCPFHPRCFKRMEICTHTRPRSVEVSKGHSVGCHLYP"
     gene            complement(2470..3315)
                     /locus_tag="PLD15_08920"
     CDS             complement(2470..3315)
                     /locus_tag="PLD15_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPB64070.1"
                     /translation="MKQLRTVRKIVTGKDAVDGAGVKLVRVIGYRDTAEFDPFLMLDA
                     FDSTNPDDYTKGFPWHPHRGIETITYLISGDIEHGDSLGNSGSILDGDCQWMTAGSGI
                     IHQEMPKASERLFGVQLWLNLPARDKMVDPQYGDIKSEDITVVDEDGSRVRIIAGRYR
                     DSNGAFEGRYVKATYLDVEVAAGRDWSFESQRDSTLFIYILQGEGLFDPTGMESITSK
                     RAVLFDEGDGFWVKAGDEGIRFLLLSGKPLKEPIAWGGPIVMNTRQELDQAFRELNEN
                     TFIKK"
     gene            3474..4412
                     /locus_tag="PLD15_08925"
     CDS             3474..4412
                     /locus_tag="PLD15_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB64071.1"
                     /translation="MEILRGKVAAAVTAIICSILWGSAFPVLKIGYEEMAIASDDISA
                     KLVFAGLRFLGAGLMVLLLSIPFGRKERQKFTAGDYLHFIALGTMQTFLLYFFFYNGL
                     SYTTGMKSSIIMAGENFLVILLAHYIYHNDRLSWRKTLGMLLGFLGVFLANFNEGFTL
                     SFVFKGDGFMLIATSIGAIGTIYAKWLSASRSPFLVSGWQLSIGGLMLLLFGLPGLKA
                     GALVFTPKGVSLLIYSMLLSAIAFSLWYALLSYHKAGEITMFRFVIPVAGVFLSAAFV
                     PGEAINSYTLLSLLLVALGIIVVNWRFSKAPAAGKH"
     gene            complement(4409..>5065)
                     /locus_tag="PLD15_08930"
     CDS             complement(4409..>5065)
                     /locus_tag="PLD15_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GCN5-related N-acetyltransferase"
                     /protein_id="HPB64072.1"
                     /translation="IEKGISLFIISGKRGLYSRFGAIEPDGFYKLRVLPGKASRVREA
                     SEEDLSRIAWLYMRRPVRYFRDLSQFSKIFATGRVMVRAARTFIGDKAYVTVIKFRDS
                     NYIVEHAGCESDVIEAARGYLQLSGEKECVLVLDSLFPSEEREKTGFEGTMKVISIDN
                     FLDQLEGYYREVMTDVEFEAFREEARKMTPAEFTRLVLLESNMKGSPIPLPVYGFDYI
                     "
BASE COUNT         1165 a         1395 c         1296 g         1209 t
ORIGIN      
        1 tgatggacgc gatgatggcc tttccgggaa ttctcttcgc cctgatgttc gtctcaatct
       61 tcggggtcgg tataagaaac acgatcatag cgataggtat catgtcgatt ccctctttcg
      121 ccaggataac caggagcgga ttcattcaac acaaagagtt cgaatacgtt aaaaacgcgc
      181 gggtcaaagg cgtctcgaac ttcaggataa tgttcagcca catacttccg aacgtgctgt
      241 ctcccataat cgtcgcggcc tcgatgggtt tttccagcgc cgtgctctcg gaggcggccc
      301 tgagttatct tggccttgga gttcagccgc ccgatcccag ctggggcagg atgctcaacg
      361 agtcgcaggt cttcataact ctcagtccgt ggtacaccat agcgccgggg atcttcataa
      421 cgctgatagt tctcggcttc aacttcctgg gcgacggtat cgccgatctc caggacaggc
      481 agcagtaggt gtaaacgtgg agcgaataat ctcagatagg gctctccttc aggtgaggga
      541 tctgaatatc tccttcacca cggctaaggg agacttcccg gcggtcaggg ggatctcctt
      601 cgatgtcggg agaaactcca tcgtaggtct ggtaggggaa tcgggttgcg gaaaaagcgt
      661 gactgccctg gccatcatga gactccttcc gggaaacgcc cggctttcgg gtacgatcga
      721 cttctccggc aggaatatag tcgcgatgca cgaggaagag ataagaaaac tgcgcggcag
      781 cgagatttcc atgatcttcc aggaaccgct tacctcgcta aatcccttgt ggaaagtcgg
      841 tagacagatc gacgagaacc tgaagatgca caccgaactg aagggcgatg ataggtttca
      901 aagtgttgta gatatcatga gagacgtggg gctgcccgac ccggagagac tataccacaa
      961 atacccccac gaactgtccg gcggaatgcg ccagaggata gccatcgcca tagccgtagc
     1021 ctgcaggccg gccctgataa tcgcagacga gccgacgacg gcgctcgacg tcactatcca
     1081 ggcacagata ctccagctgc tgaaggatat aaacagagag aagggctcct ccataatctt
     1141 catttcccac aacttcggca taataagaga gatatgcaca tcgatctgcg tgatgtacgc
     1201 cgggcatctt gtggagatcg ccccgaccga aaagctcttc tgtgaaccgc gtcatcccta
     1261 cacgataggt cttatgaagg caattccgaa aaccgattcc aaaggcaagg aactttacga
     1321 catccccgga aaagtgccgt cgataaccga tacgcttccc ggctgtccct tcgagcccag
     1381 gtgtgaatac gccttcgaaa ggtgtaaaag ggagttgccg caattcgaag ttctcggaga
     1441 gcaacacgag tcgagatgct tcctggccgg tgagctgcga tgagcggcat aattctcgaa
     1501 gtcagagacc tcgtgaaaca ctacccgctg cgaaggacga agctgctgga gagcccgggc
     1561 agagtcaggg ctctcaacgg tgtctccttc gccatagcga ggggagagtc cttcggactg
     1621 gtcggggaat cgggctgcgg aaagacaacg gccggccaca cgataataaa gctgctcgag
     1681 ccgacagatg ggaagatatt tttcgagggc aaggatataa caactatcaa gggcgagaga
     1741 ttgaaagatc tcaggaggaa gatccagata atctttcagg ataccgccgg ctctctcaac
     1801 cccaggaaaa ctgtgaagtg gatcctgagt gagcctttac gcgcacaggg tgtgagaaac
     1861 cgcctggaga tcgaaaggac aatccgggaa agcctggagc tggtggatct ggacgagagt
     1921 ttcctggatc gttacccaca tgaactgtcg ggcgggcaga agcagaggat cggcatactg
     1981 ggcgctttga ttctcgagcc ggaattcatc atagcggatg aacccgtttc ggcgctggac
     2041 gtctccatac aggcccagat actcaacctg atgaaaaacc ttcaaaagag gctttcgctg
     2101 acctacctct tcatatccca cgatctcggc gtagtacatt tcttctgcga caggatagcc
     2161 gtgatgtacc tcggagagat cgttgaaatg gctgaagcga cggagctgta cgtcaatccc
     2221 tgtcatccat acacgcagtc gctactctcg gcgataccgg gagagaatgc cgcgagccag
     2281 cggataatcc ttcaggggga cgcacccgat ccgaccaacc ctccacaggg ctgtcccttc
     2341 catcccaggt gcttcaaaag aatggagata tgcactcaca ccagaccaag gagcgtcgag
     2401 gtatcgaagg ggcattcggt tggctgtcat ctctacccgt gaaatggagc gcctccccgg
     2461 acggacggtt cacttcttga taaacgtgtt ttcgttgagt tctctgaaag cctgatcgag
     2521 ctcctgacgg gtgttcatca cgatgggacc tccccatgcg ataggttctt taagtggttt
     2581 gccagaaagc agcaaaaacc tgatcccttc atcgccggcc ttgacccaga atccgtcgcc
     2641 ttcgtcgaac aaaacggccc gtttgctggt tatggattcc attcctgtcg gatcgaagag
     2701 tccctcgccc tgaagaatat agatgaacag agtcgagtct ctctggcttt cgaaagacca
     2761 gtctcttccg gccgcgacct cgacatccag ataggtagct ttcacatacc ttccttcaaa
     2821 tgcgccattc gagtctctgt aacgacccgc gatgatgcgt actctgctcc cgtcttcatc
     2881 tacgacagta atgtcctcgc tttttatgtc gccgtactga ggatctacca tcttgtctct
     2941 ggcgggcaga tttagccata gctgaacacc gaagagcctc tcgctcgcct tgggcatctc
     3001 ctgatggatt atccccgatc cggccgtcat ccactggcaa tcgccgtcga gaatgctgcc
     3061 gctgtttcca aggctgtctc cgtgctctat atccccgctt atgaggtatg ttatcgtctc
     3121 gataccacgg tgtggatgcc agggaaagcc tttcgtgtaa tcgtccgggt ttgtggagtc
     3181 gaaggcgtcc agcataagaa aagggtcgaa ctctgcggta tctctataac cgatcaccct
     3241 taccagtttc accccggctc catcgaccgc gtccttgcct gtaactatct ttcgcacggt
     3301 gcgcaattgc ttcataggaa tcttctcctc tcataaatcc tattacaatg ataccatcgg
     3361 tgggtatcta acaaacgaag cggccgtccc ccaacctacc acgacggtta tacggagctg
     3421 tatcgtcagc tttccgcatc aactatttaa tggtgaaggt gtaaactcgg ttcatggaga
     3481 ttctaagggg taaagtcgcg gcagccgtga cggctataat atgttcgata ctctggggaa
     3541 gcgccttccc ggttctcaag atcggttacg aagagatggc catcgcttcc gacgatattt
     3601 cggcgaagct ggttttcgcc ggtctgcgtt ttttgggcgc cgggctgatg gtactgcttc
     3661 tttcgattcc cttcggcaga aaggaaagac agaagttcac ggccggggat tacctccact
     3721 tcatagctct gggtacgatg cagaccttcc tgttgtattt cttcttttac aacggtctca
     3781 gttacaccac cggcatgaag tcctcgataa tcatggccgg agaaaacttt ctggtcatac
     3841 tcctcgccca ctatatatac cacaacgacc gtctcagctg gagaaaaact ctcggaatgt
     3901 tgcttggctt tctgggcgtc tttctggcca atttcaacga aggtttcacg ctgagcttcg
     3961 ttttcaaggg cgacggtttc atgctgatcg ccacgtcaat aggggccata ggaacgatct
     4021 acgccaagtg gctttcagcc agcagatcac ccttcctggt gtcgggctgg cagctgtcga
     4081 tcggcggtct tatgttgctt ctcttcggtc ttcccggcct gaaggccggc gcgctggtct
     4141 tcacaccgaa aggagtctca cttttgatct actctatgtt gctttcggcg atcgccttct
     4201 ctctctggta cgccctgctc agttatcaca aggccggtga gataaccatg ttccgtttcg
     4261 tcatacccgt cgccggagtc ttcctgtcgg ccgccttcgt tcccggtgaa gcgataaact
     4321 cctatacact actctcgctt ctcctcgtcg ctttgggcat catcgtggtc aactggaggt
     4381 tttcaaaagc tccggctgcc ggaaaacact agatgtaatc gaaaccatag acgggcagcg
     4441 gaatagggga gcccttcata ttactctcaa gcagcaccag tctggtgaat tcggccggag
     4501 tcatttttct ggcctcctct ctgaaggctt cgaactctac gtccgtcatt acttccctgt
     4561 aataaccttc gagttgatcg agaaagttat caatggatat gaccttcatc gttccttcga
     4621 agccggtctt ttctctctcc tcgctaggga agagactgtc aagaacaaga acgcattcct
     4681 tctctccgct caactggaga tagccccttg ccgcctcgat aacgtcgctc tcgcatccgg
     4741 cgtgttcgac gatgtaattg ctgtcacgga actttatcac cgtgacgtaa gctttatcgc
     4801 cgatgaaggt tctcgccgct ctaaccatca cccggcctgt ggcgaaaatt ttactgaact
     4861 gcgaaagatc cctgaaataa cgtacgggcc tcctcatgta gagccaggcg attctcgaaa
     4921 gatcttcttc gctcgcctct ctgacacgtg aagcctttcc cggcaagact ctcaacttgt
     4981 agaagccgtc cggctcaatg gctccgaacc tgctgtacag accacgctta cccgagatta
     5041 taaagagcga tatgcccttc tcgat
//
LOCUS       DAOOLY010000182         5061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_19887_length_5061_cov_3.679984, whole genome shotgun sequence.
ACCESSION   DAOOLY010000182 DAOOLY010000000
VERSION     DAOOLY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19887_length_5061_cov_3.679984"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLD15_08935"
     CDS             <1..749
                     /locus_tag="PLD15_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPB64073.1"
                     /translation="DSDYRTGQNYVGETIAWQRERIHFACPKPEDLADLMEGLVTAHK
                     RMDASDVSPVVHAAVIAYGFVFLHPFEDGNGRIHRFLIHNILARRGFTPKGVMFPISA
                     SMLKNPEDYDASLEAFSRHIMPLVDYSLDKEGSMTVHNDTARWYRYIDMTPQVEALFK
                     FIDQTIEEELPRELAFLASYDETKKAIQEIVDMPDRLIDLFIRFCLQNNGRLSAQKRK
                     SHFDFLSDEEIAHIEQAVLAVYGKRTLNDD"
     gene            1031..2437
                     /locus_tag="PLD15_08940"
     CDS             1031..2437
                     /locus_tag="PLD15_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPB64074.1"
                     /translation="MKKSFISVFLFLLLAVVILLITGCPWFWKEGYVKNEPPEVNKVS
                     GPDGEISENSSTFTWTGKEYDGTITKYEYRKDEGSWESNATNTSYTWNGYSEGPHKFE
                     VRAKDDNGKYSTAVSWIFTYNKANQPPVVAKVSGPNGETSENGSTFTWTGSDPDGIIE
                     NYLFRKDDWSWESNGTGTSYTWNGYSEGPHKFEVRAQDNEGAYSNIVSWSFAYSTGVG
                     EMVLVEGGTFMMGDEFGDLVDFCRPVHEVTLTYDFYIGKYETTFDEYDAFCVVTGRSK
                     PNDEGWGRGSRPVIYVSWWDAIAYCNWLSEKEGLPKAYDTIGNLLDKDGKVTTDITKV
                     PGYRLPTEAEWEYAARGGNDSNGYMYSGSDDVGEVAWYYSNSEDKTHEVGKKAPNELG
                     IYDMSGNVWEWCSDWWYDYTETTKINPYNGTAGSYRVERGGSWYKVATCARVANRGNY
                     SPTETYSGLGFRICRTVP"
     gene            complement(2490..3083)
                     /locus_tag="PLD15_08945"
     CDS             complement(2490..3083)
                     /locus_tag="PLD15_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HPB64075.1"
                     /translation="MKCVVEGGGEYFVQIRTIYPTKIYSNSKGDRRIVVEGRPGPAEK
                     ARLISLWEEFNGHSFKGVNIFVDGSCRGQVSSYAAVIIENGLVIGMISGCFRDGAGMR
                     NVAGEIVAVEKAVEFCLKKSITAVKIHYDYEGLYGWAEEYWQAKTPRTIEYRKRMREI
                     SESLSIKWVKVKAHSGNSYNEMADKLAKKALQECKMV"
     gene            complement(3155..3925)
                     /locus_tag="PLD15_08950"
     CDS             complement(3155..3925)
                     /locus_tag="PLD15_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPB64076.1"
                     /translation="MWYPLALIVPIFFIFGLVFGSFSNALIYRIPSKEYTIFKPRRSI
                     CPSCKHELAWKDNIPLFSYLTLKGRCRYCGSKISPRYPLVELMTASLYALNAALFPLS
                     EAISLSLMVTGLVVSAFIDLEHYMIPDSGVILVGLGSFFWAFFRGRFPENLIGALIVT
                     GAMVAFFLIANQFRKDSFGFGDVELLAVLSLATGILGSLYTIMIAAFVALASYAIASA
                     VKRRKFDRLAQLPFGPFIASAGYLTVLVLDLIEGLYAF"
     gene            complement(3910..4980)
                     /locus_tag="PLD15_08955"
     CDS             complement(3910..4980)
                     /locus_tag="PLD15_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB64077.1"
                     /translation="MELIRLKYDGNIYSITDTLPFSVAILDQIYDGDLNLCLEDLGGT
                     KIEPDLETLKSLIAAFEDIVEPEIYAPIEYLEFNEYMNECGLTVKNFARGTFGGFQDK
                     ILSIADRGDYESAQKFLDLLMDANVDKATLCELKGNIYLEEGNEEEGIRWLQQALMID
                     PSLLSAHSFLGQTYYNRGEYDKAAQYWNREISIAPDHLVTYFMLTDAYIQAGRIDEAM
                     NVLRTLSQRDPSSILTKAEMVELCQKAGDFEQAGKIEEEILNSRPVYINDVEVWAKTQ
                     FRYGRFDTVQEQVLEFLKKEPEKPELKMLLVVTFMKLKNCEEARRLMKSFEKGQLWYF
                     YGKKELFNEFLTEEERRQCGIL"
BASE COUNT         1309 a         1247 c         1273 g         1232 t
ORIGIN      
        1 gagactccga ttaccggacg ggccagaact atgtcggaga gacaatcgcc tggcagaggg
       61 aaagaataca tttcgcatgt ccgaagcctg aggatctggc cgacctgatg gagggcctgg
      121 taaccgctca taagcgtatg gacgccagcg atgtgtcccc cgtggttcac gccgccgtta
      181 tcgcctacgg gttcgttttt ctgcacccgt ttgaagacgg caacggtcga attcaccgtt
      241 ttctgatcca caacattctt gcccgccggg gctttacgcc caaaggcgtc atgtttccca
      301 tatcggcgtc gatgctgaag aaccctgaag actatgacgc ctctctggaa gccttctcac
      361 gtcatattat gcctctggtg gactactcgc tcgataaaga aggcagcatg accgttcaca
      421 acgataccgc caggtggtac cgctatatcg acatgacgcc ccaggtggag gcgctcttca
      481 aattcatcga tcagaccata gaggaggaac tcccccggga attggcgttt ttggccagct
      541 acgatgagac aaagaaagcc atccaggaaa tcgttgatat gcccgaccgg ttgatagacc
      601 tcttcatccg cttttgcctg cagaacaacg gcaggctttc tgcacagaaa cgcaagagcc
      661 acttcgactt tctgtcggac gaggaaatcg cacatattga acaggccgtt cttgcagttt
      721 acggaaagag aaccttgaac gatgattgat ggcgccccgc gaaactcatg acgatcggcg
      781 acactcatct ccgggccgga agataagttt atgcgtgcca gactagacga ctgatagaaa
      841 gcgatgaaca gggcttatcc accgataaac gaatcgcaga aattgaaatt cacacgagtg
      901 aatattcctt tgtgcagtat gtaataactg ctatattagt cttatgagtg cattgcaccc
      961 cttgcagtgt attgtctgag tcttcaagtg tttacttcga gtgcgaggcc tcgcttatgg
     1021 aggtggggta atgaagaagt cgtttatttc ggtttttttg tttttactgc tagcggttgt
     1081 aatacttctt ataacaggat gtccctggtt ttggaaggaa ggatatgtta agaacgaacc
     1141 tccagaagtt aataaagtga gcggtccaga cggagagatc tcagaaaaca gcagcacctt
     1201 cacctggact ggaaaggaat atgacggaac gataactaag tacgaataca gaaaagatga
     1261 agggagctgg gagagtaacg ccacgaatac gagttacacc tggaacggtt actcggaagg
     1321 gcctcacaag tttgaggtca gggcgaagga tgataatggg aagtattcga ccgcagtgag
     1381 ctggatcttt acatataaca aagcaaacca gccacctgtc gtggcgaagg tcagcggtcc
     1441 aaacggagag acctcagaaa acggcagcac cttcacctgg actggaagtg accccgatgg
     1501 aattatagaa aattacttat tcaggaagga cgactggagc tgggagagta acggtacggg
     1561 tacgagttac acgtggaacg gttactcgga agggcctcac aagtttgagg tcagggcgca
     1621 ggacaacgaa ggtgcttact ccaacatagt aagctggagc tttgcatatt cgacgggagt
     1681 cggagaaatg gtactcgtcg agggtggaac tttcatgatg ggcgacgaat ttggagactt
     1741 agtggatttt tgcagaccag ttcacgaggt aactctcaca tacgacttct acatcggcaa
     1801 gtatgaaact actttcgacg aatacgacgc cttctgcgtg gttacaggcc ggagcaaacc
     1861 gaacgacgaa ggttggggaa ggggaagcag acctgtaatc tacgtgagct ggtgggacgc
     1921 gatagcatac tgcaactggt tgagtgagaa agaaggactt cccaaagcgt acgacaccat
     1981 cggaaatcta ttggacaaag acggaaaagt aacgacagac ataacaaagg tacccggata
     2041 cagattgcct actgaagcgg aatgggaata cgcagcccgt ggcggtaacg atagcaatgg
     2101 atacatgtat tccggtagcg atgatgtagg tgaggtggcc tggtactatt cgaattccga
     2161 ggataaaact cacgaagtcg ggaagaaggc tcccaacgag ctgggtatct acgatatgtc
     2221 cggaaatgtg tgggagtggt gttccgactg gtggtacgat tacacagaga caacaaagat
     2281 aaatccatac aacggtactg ctggttccta ccgggtagag cgtggcggca gctggtacaa
     2341 agtcgcgacg tgcgcacgtg tggcgaatcg cggcaactac tcgcccactg aaacatacag
     2401 cggcctgggt tttcgtattt gcaggacggt gccctgatta cactttagcc tttttactac
     2461 gggatgacag tgcaagagta ccatggaaat tacaccatct tacattcctg cagtgctttt
     2521 tttgcgagtt tatcggccat ttcgttgtaa gaattcccac tgtgtgcctt tactttcacc
     2581 cactttatcg agagactttc tgagatctct ctcatgcgct ttctgtactc gattgtgcgc
     2641 ggtgtcttcg cctgccagta ttcctctgcc catccataaa gcccttcgta atcgtagtgg
     2701 atcttcactg ccgtgatgga ctttttcaaa cagaactcca cggctttttc gaccgccact
     2761 atctctcccg cgacgttccg cataccggcg ccatccctga aacagcccga gatcatccca
     2821 atcactaatc cgttttcaat aatgactgcg gcgtaagagg atacctggcc acggcaactc
     2881 ccgtccacga agatattaac ccctttgaag ctgtggccgt tgaactcctc ccaaagggag
     2941 atcaaccggg ctttttccgc cggtccgggt cggccttcga ccacgatcct tctgtctccc
     3001 tttgagttac tgtatatttt agtgggatat atcgttctga tctgtacgaa atactcaccg
     3061 ccaccctcta cgacacactt cattcctctc tctgtgagaa aagtacagaa ttcatccgct
     3121 gcttcgttca gaagagttct ccagtctttt gtactcaaaa tgcgtacagt ccttctatga
     3181 ggtcaagtac cagtaccgtc agatacccgg ccgatgcgat gaacggaccg aagggaagtt
     3241 gggccaacct gtcgaacttt cgccttttga cggccgatgc gatcgcgtaa gaggccagtg
     3301 ctacgaaggc tgctatcatg atagtgtaca gagagcctag tatccccgtg gccagtgaga
     3361 gcaccgccag aagctcgaca tcgccgaaac cgaaactatc cttccggaac tggttggcga
     3421 taagaaagaa agccaccatg gcaccggtta cgataagcgc tcctataaga ttctccggga
     3481 aacggccccg gaagaaggcc cagaagaaac tgcccagccc caccagtatc actcccgaat
     3541 cgggtatcat gtaatgctcc agatctatga aggcagaaac aactagcccg gttaccataa
     3601 gagaaagcga aatcgcctca gacagtggaa agagcgcggc gttgagcgca taaagcgagg
     3661 ccgtcataag ctctacgaga gggtatcggg gcgatatttt cgagccgcag tacctgcatc
     3721 ttcctttaag ggtgagataa ctgaaaagcg ggatgttatc cttccaggcc agctcgtgct
     3781 tgcacgaagg acatatgctt cttctgggct tgaatatcgt gtactccttc gatggtattc
     3841 tatagataag agcgttagaa aaactaccga aaaccagccc aaaaataaaa aaaatcggta
     3901 cgatcagcgc tagaggatac cacactgtct tctctcttct tctgtgagaa attcgttgaa
     3961 aagctccttc tttccgtaga agtaccagag ctggcccttc tcaaaggatt tcatgagcct
     4021 tctggcttcc tcgcagttct tgagtttcat gaaggttacc accaaaagca tcttcagctc
     4081 gggcttttcc ggttctttct ttagaaactc cagaacctgt tcctgaacag tgtcaaagcg
     4141 gccatatctg aactgcgtct tggcccaaac ttcgacgtcg tttatataga caggtcggga
     4201 attgagtatc tcctcttcga tcttgcctgc ctgttcgaaa tcgccggcct tctgacacag
     4261 ctcaaccatt tcggccttgg tgaggatact tgaggggtca cgctgggaga gcgtcctgag
     4321 aacgttcatc gcctcgtcta ttctaccggc ttgtatatag gcatcggtca gcatgaaata
     4381 cgttactaga tggtcggggg ctatcgagat ctcacgattc cagtattgtg cggccttgtc
     4441 atattccccc cggttgtaat aggtctgacc gaggaaggag tgtgcactca aaagcgaggg
     4501 atcgatcata agggcctgct gaagccatct tataccctcc tcttcgtttc cttcttcaag
     4561 atagatgttg cccttgagtt cgcacagagt ggctttgtca acgttcgcat ccattaaaag
     4621 atcgaggaac ttctgtgcgc tttcgtaatc gcctcgatcg gcgatggaaa gtatcttgtc
     4681 ctggaaaccg ccgaaagtac ctctcgcgaa gtttttcacc gtgagtccgc actcgttcat
     4741 gtactcgttg aactccaggt actctatggg ggcgtaaatc tccggctcga caatatcctc
     4801 gaaagctgct attaaagact tgagagtttc gaggtccggt tcgatcttcg tcccgccgag
     4861 atcctcaagg cagagattga ggtctccgtc gtaaatctga tcgagaatcg ctaccgagaa
     4921 aggcaaagta tccgttatag agtagatgtt accatcatac ttgaggcgaa tcaactccat
     4981 ttccccacct cttttccgtg tttcttagaa tataccatgt ttgcgaccgg ataacactaa
     5041 cgttgcctta tatggtacaa a
//
LOCUS       DAOOLY010000183         5016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20128_length_5016_cov_4.530337, whole genome shotgun sequence.
ACCESSION   DAOOLY010000183 DAOOLY010000000
VERSION     DAOOLY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20128_length_5016_cov_4.530337"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLD15_08960"
     CDS             <1..273
                     /locus_tag="PLD15_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64078.1"
                     /translation="IGRSLMANPDLIMMDEPSLGLAPIVVEEVFKAIQSLNNKGVTIL
                     LVEQNAAKALEISRYAYVLETGCVTLQGDAKKLLESEEVRKVYLGI"
     gene            complement(281..922)
                     /locus_tag="PLD15_08965"
     CDS             complement(281..922)
                     /locus_tag="PLD15_08965"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB64079.1"
                     /translation="MELIYSAVFAYMIGSIPTAYLIGRVFFGFDIRNRGTGNMGASNM
                     VSVMGWKIGILTGAIDFMKGMGTILIIKGLDPHWTYQMLFVAATFATIGHMYPVFLGF
                     KGGKGTATLAGGLIGLDYQVALICMTILLVFIATTDYSAPGVIAVSSVMIFLFRIFSY
                     RWGISLMLLPIALIMIARNLENLHRISSGKETGLKAYIRKKKKERSPTEGAQG"
     gene            complement(936..1703)
                     /locus_tag="PLD15_08970"
     CDS             complement(936..1703)
                     /locus_tag="PLD15_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HPB64080.1"
                     /translation="MIEVVLFNALVVLAYMSIFFLIASAVKDNSIVDVAWGTGFVLIA
                     YFTLLFFGEFNLRQFLVTIAVTLWGLRLAVHIFRRNKGRGEDFRYAAMREKWGRYVLI
                     RSFLQIYVLQGFFMLVVAYPVMLVNSTPGPSFSWLDFLGLSIWIAGFLFEAVADSQLR
                     EFVKTKRPGEIMKSGLWRYSRHPNYFGEAVQWWGIFIVALSLDGGWWTIVSPILITFL
                     LRFVSGVPYLEKKYRQYQQFREYMKETNAFIPWFPKK"
     gene            complement(1713..3530)
                     /locus_tag="PLD15_08975"
     CDS             complement(1713..3530)
                     /locus_tag="PLD15_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB64081.1"
                     /translation="METKILNVIDGPTLFNAFFSGARAVIKEQGYLNKINVFPVPDGD
                     TGTNLASTMRSIIELTAVSQSVGKTSRSIADAALMGARGNSGAIFAQYIHGLSNAIGN
                     KESLDSGSFAKAVQEAVPYAYDAMSTPVEGTMLTVMKDWAEALNSAGNSENSFLEMIT
                     RSNEVAKKSLEETPEKLDVLKKAGVVDAGARGFVAFVEGIKIFLSGKTMEMEDEVIPD
                     IELRNHVHVDETNIQFRYCTEAIIEGTGIDHASLKRDASSFGDSVVVVGSDKRTRVHI
                     HTNEPADLFYRLGKYGSITQQKVDDMEMQYRVSQKRKYPIALVTDSVCDLPREIIDYY
                     QIHVVPLFLNFGESHYLDKITIKPDQFYTMLDSTETYPVSAQPGMNSFQNLYHFLATH
                     YDSVIAIHVSDKLSGTWNSSLKAAERLKGKKISVINSRHVSGSFGLLVLRAAQLIEKG
                     MDHDEIVAAIESLTKNSRIFVSVNTLKYMVKGGRVSPTKGLLAKIMNLKPIVSVDEEG
                     NSKLYGKAFSRLGNMKKILGFIGELNRKCRVENYNITHARAPKQARAFEQSLTKLLGK
                     KPEFIMEVSPVVGISAGVGAVSVSVLCERNTWSEEIKVR"
     gene            complement(3711..3911)
                     /locus_tag="PLD15_08980"
     CDS             complement(3711..3911)
                     /locus_tag="PLD15_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPB64082.1"
                     /translation="MTGTVKWFNGTKGYGFITKDEGGDVFVHFSAIEMDGFKTLDEGQ
                     RVEFEVEDGPKGPQAAKVRIAK"
     gene            complement(4089..>5016)
                     /locus_tag="PLD15_08985"
     CDS             complement(4089..>5016)
                     /locus_tag="PLD15_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64083.1"
                     /translation="MRNLESEEHPEETTSTQEQKNLEWLQYGQTTAEPESPGRDMLRA
                     PRIVDEAPPEAGVIESIIISVFYTLSLVLVASIAWKTFQITRGFAKLIPPFLILISLL
                     IFMIGGFMYLRSLPLADNIEFLSSGDTLSPAPSSNVVEVESSASVDTAPEESALAGIL
                     FETLRWSSFAAIALLSIALALAIVFVTAGSDEGRKRGGDHEKTLKGPQDSQKLPDRQV
                     PPLKYDRKFILDGYRWLRMNFFKDYVQLTPYELLEEIDQEKRGAFLEATSIYVPVKYG
                     QIDPSREECVKFHSLLVELFQTREAGSSTKKA"
BASE COUNT         1200 a         1316 c         1179 g         1321 t
ORIGIN      
        1 ataggcagaa gcctgatggc caatcccgat ctcataatga tggatgaacc ctcgctcgga
       61 ctggctccga tcgtggtgga agaggtcttc aaagcgatac agtctctcaa caacaaaggt
      121 gtcacgatac tgctggtgga gcagaacgcc gcaaaggctc tcgaaatatc ccggtacgct
      181 tatgttctgg aaaccggatg cgtgacactg caaggggatg caaaaaagct cctcgaaagt
      241 gaagaggtta gaaaggttta cctgggtata tgaaagagaa tcacccttga gccccttcgg
      301 tggggctcct ttcttttttc ttctttctaa tgtaggcttt cagaccggtc tcttttccgc
      361 tggatatcct gtgaaggttc tctagattcc tggcgatcat tatcaatgca ataggaagaa
      421 gcatcaacga aattccccag cggtaactga atatcctgaa aagaaaaatc ataaccgaag
      481 atacggctat cactccggga gccgagtaat cagttgtggc tataaacacc agcagtatgg
      541 tcatgcagat caaggccacc tggtaatcta ggcctatcaa ccctccggcg agcgtggccg
      601 ttcccttacc gcctttgaat cctaagaaaa ccgggtacat gtgtccgata gtcgcgaagg
      661 ttgcggctac gaagagcatc tggtaagtcc agtgcgggtc taaccctttt attattagta
      721 tagttcccat acccttcatg aaatctatcg ctccggtcag aattcctatc ttccagccca
      781 tcacagaaac catgtttgac gcgcccatgt tacccgttcc cctatttctg atatcgaacc
      841 cgaaaaaaac tctcccgatc aggtaggccg tgggtatgga accgatcata tacgcgaaga
      901 ccgcagagta tataagttcc atctgatttc accctctact ttttcgggaa ccatggaatg
      961 aaggcgttgg tttctttcat gtattccctg aattgctggt actgtcggta cttcttttcg
     1021 aggtaaggga cgcccgaaac gaatctcaag agaaaggtta ttagaatcgg actaacgatg
     1081 gtccaccatc cgccatccag tgagagtgca acgataaaaa tcccccacca ttgaacggct
     1141 tcaccgaagt agttggggtg ccgggaatat ctccagaggc cgctcttcat tatctcgccg
     1201 ggtcttttgg tcttcacgaa ctctctcagc tgactgtcgg caacggcttc gaagaggaaa
     1261 ccggcgatcc agatcgacag gcccagaaag tccagccagc tgaaggatgg gccgggcgtg
     1321 ctgttcacaa gcataaccgg atacgccaca acgagcatga aaaagccctg aaggacatat
     1381 atctgcagga aggagcggat caagacatat ctcccccact tctccctcat cgccgcgtac
     1441 ctgaaatctt cacccctgcc tttgtttcgc ctgaaaatgt ggactgccag tctcaacccc
     1501 cagagagtta cagcaatcgt taccagaaac tgtctgaggt tgaactcacc aaagaagaga
     1561 agggtgaaat aggcgatcaa cacaaacccc gttccccagg cgacatcgac aatcgaatta
     1621 tccttcactg ccgaggcgat caggaagaag atactcatat aagcaagtac cacaagagcg
     1681 ttaaacagaa cgacttcgat catacagact cctcagcgta cttttatctc ttccgaccag
     1741 gtgtttctct cgcacaacac cgaaacgctc accgcgccga ccccggcgct tattccgacg
     1801 accggggaga cttccattat gaattccggc tttttcccca gcagcttcgt gagactctgc
     1861 tcaaaagccc tggcctgctt cggtgcgcgg gcatgcgtta tgttgtaatt ctcgaccctg
     1921 cactttctgt tcagttcacc gatgaaaccc agtatcttct tcatgtttcc gagcctgctg
     1981 aaggccttcc cgtaaagttt cgagttgccc tcttcatcca ccgatacaat cggctttagg
     2041 ttcataattt tggccagcag gcccttcgtc gggctgactc ttccaccctt gaccatgtat
     2101 ttcaaagtgt tcacactcac gaagatcctt gagttctttg tgagcgattc gatggctgcg
     2161 acaatttcat cgtggtccat tcctttttct atgagctggg cggccctcag gacgagcaat
     2221 ccgaaggagc cggagacgtg cctggaattt attacgctga ttttctttcc tttaagtctt
     2281 tctgccgctt tcagacttga attccaggta ccgctcagtt tatcggaaac atggatggcg
     2341 atgactgaat cgtaatgtgt cgccagaaag tggtagagat tctggaagga attcatcccc
     2401 ggctgggccg aaaccggata ggtctcggtg ctgtcaagca tcgtatagaa ctggtcgggc
     2461 ttgatggtta tcttgtccag ataatgactt tccccgaaat tcagaaacag cggcacaacg
     2521 tggatctgat aataatctat gatctctctc ggcagatcgc acaccgagtc ggtgacgagt
     2581 gcgatggggt attttcgctt ctggctgact ctgtactgca tctccatgtc gtcaactttc
     2641 tgctgcgtga tgctgccgta ttttccgagc ctgtaaaaga gatcggccgg ctcgttggtg
     2701 tggatgtgaa ccctcgtgcg tttgtcggag ccaaccacta cgacggaatc tccgaaagaa
     2761 ctcgcatctc tcttcagaga ggcgtggtcg attcccgttc cctctattat cgcttcggtg
     2821 cagtacctga actgtatgtt ggtctcatcg acatggacgt ggttgcgcag ctcgatatcg
     2881 ggaatgacct cgtcttccat ctccatagtc ttgccgctca ggaatatctt tattccctcc
     2941 acgaaggcca cgaaacccct ggccccggca tctacgacac cggccttttt caatacatct
     3001 agcttttcgg gcgtttcttc cagagatttc ttcgcgactt cgttggatct ggttatcatt
     3061 tcgagaaagg agttttcgga gtttccagcc gagttcaacg cttctgccca gtccttcata
     3121 actgtgagca tcgttccctc taccggtgtg gacattgcat cgtaagcgta agggacagcc
     3181 tcctgaacgg ctttggcgaa acttccgctg tcgaggctct ctttgttgcc gatcgcgttg
     3241 ctaagcccgt gtatgtactg ggcgaagatc gctccggaat tgcctctggc acccatgaga
     3301 gcagcatcgg cgatcgatct ggaagtctta ccgacggatt gagagacggc cgttaactct
     3361 attattgatc tcatcgtaga tgccagattg gttcccgtgt ccccgtcagg aaccggaaag
     3421 acatttattt tgttgaggta accctgctcc ttaataacgg ctctggcgcc agagaaaaat
     3481 gcgttgaaaa gtgtcggtcc atcgattacg ttcagaatct tggtctccat gttctcctcc
     3541 tgtcatgaca ccgcttcatg aatatgttag cacaacagag attggagtac agcgcactgt
     3601 atatagtggt tttggctttt tagagtgctc cgggagggcc ggtggagtgt cagaagaaaa
     3661 aaatcgcccg ctgtcaagcg ggcgatggga ttgcttatat agacattcga ttacttcgca
     3721 attctaactt ttgctgcctg aggacccttt gggccgtcct caacttcgaa ctctactctc
     3781 tgaccttcgt caagagtctt gaagccatcc atttcgattg cggagaaatg aacgaagaca
     3841 tccccacctt cgtccttagt aataaagccg tacccctttg ttccattgaa ccatttcaca
     3901 gttcctgtca tacttaagtg ttcctcctta aaattacctt gcaccacttg caaagcgata
     3961 gtgtccttct ggacagattg attataacac acttaattca ataaattaca agtacccggt
     4021 ccgaaataaa aagaatattt ttgtcattcc ctttcccaaa ctaccagttc ccttctccgg
     4081 atgcttcgtt acgctttttt ggtcgagctt cccgcttctc tcgtctggaa aagttccacc
     4141 agaagcgagt ggaacttcac acactcttcc ctggagggat cgatctgtcc gtatttgacc
     4201 ggtacataga tcgatgtagc ctccaggaac gctcctcgct tttcctgatc gatctcctcg
     4261 agcagttcgt agggtgtgag ctggacgtaa tctttgaaaa agttcatccg cagccatctg
     4321 tagccgtcaa ggatgaattt tctgtcgtat ttgagaggcg gcacttggcg gtccggtagt
     4381 ttttgactgt cttgaggtcc tttaagggtt ttttcatgat cgccaccgcg ctttcggcct
     4441 tcatcgctgc ccgcagttac gaaaactatc gcgagtgcga gggcgatcga aagaagggcg
     4501 attgcggcga atgaagacca cctcagagtt tcgaacagga tcccggccaa cgcggactct
     4561 tcaggagctg tatcgacact cgcggagctt tcaacctcta cgacgtttga agacggagcc
     4621 ggagacagag tgtctccgct cgacaaaaac tcgatgttat ctgccagggg aagcgatctt
     4681 agatacatga atccccctat catgaagatc aaaagagaga tcaatatgag aaaaggcggt
     4741 atcagcttag caaatccacg cgttatctgg aacgtcttcc acgctatcga agccactagc
     4801 actaacgaaa gggtatagaa caccgatatg attatcgatt ctataactcc cgcttccggt
     4861 ggagcttcgt caacaatacg gggagcgcga agcatgtctc tccccggact ctctggttcg
     4921 gccgttgtct ggccgtactg aagccactca agattttttt gctcctgagt ggaagtggtt
     4981 tcttcgggat gctcctcgct ttccaaattc ctcata
//
LOCUS       DAOOLY010000184         5012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20150_length_5012_cov_4.511398, whole genome shotgun sequence.
ACCESSION   DAOOLY010000184 DAOOLY010000000
VERSION     DAOOLY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20150_length_5012_cov_4.511398"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1182)
                     /locus_tag="PLD15_08990"
     CDS             complement(196..1182)
                     /locus_tag="PLD15_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64084.1"
                     /translation="MKIIETKELKKYFPIKAGVFLQVVGHVKAMESVNFHINRGETVG
                     VVGESGCGKSTLGRTIVKIYEPTGGRITYYDNDAGEHDITKGLDKKIRLKFRRDIQMI
                     FQNPFDSLDPRMTIRDVIREPIEAHNLVPKNEIDGYVAELLMKVGLYPEYSQRYPHEF
                     SGGQRQRIAVARSISVKPRLVICDEPTSALDVSVQSQIINLLQDLKAENDISYIFISH
                     NLDVVHHMSDRILVMYLGNVVESAGATDLFDNPAHPYTGALMASIPGWDPLDRKLQNI
                     RLEGEPPSPINPPPGCAFHPRCPYRMDRCDKEKPPVVEIAPGHETACWLHTK"
     gene            complement(1179..2150)
                     /locus_tag="PLD15_08995"
     CDS             complement(1179..2150)
                     /locus_tag="PLD15_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64085.1"
                     /translation="MSGILEVKNLKTYFRTPDGLVKAVDDISFELQKGEILALVGESG
                     CGKSVTVQTVMGLIKVPPARVEGQILYREKNLVGLSNKEYKNIRGKEISMIFQEPMST
                     FDPLYTIGYQLSEVAKAHMPWDDARTREEIISVLRKVNIPEPEKRYREYPHEMSGGML
                     QRIMIAMALITGPEIIIADEPTTALDVTIQAQVLNIMRDLQSEFNGSIIFITHDLGVV
                     SEIADRVHVMYAGKIVEKAGVNELYKKPAHPYTKGLLNSRVKRDFKGQKLPYIEGYVP
                     RAYEFPEGCRFNPRCPHAMEICKKDPPEMLVSENHTAACWLYGEGGI"
     gene            complement(2147..3238)
                     /locus_tag="PLD15_09000"
     CDS             complement(2147..3238)
                     /locus_tag="PLD15_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64086.1"
                     /translation="MQSTKKRIVRNFFKHKLGVIGLVALGIMYLLVILSDFISPYNYV
                     TSHKNFVYAPPSKIRFFDEDGKWHGPFVHPMVKVRNPVTYRLEFSEDKSKIVPIKLFV
                     RGEEYKLWGIFKTDIHLFGVEESSDKAMVLLFGADRFGRDLFSRVLIGGKVSMTVGLV
                     GTLISVFIGAIVGALSGYYGGLVDVLIQRFIELLRSFPRIPLWLALATILPPSWPSTW
                     VYFGIVIVLSLIGWMGVARVERGMVLSLREKEFVLAARVSGVSNMKIITRHLIPNTIS
                     YLIVVSTLSIPGMILGESAISFLGLGIKEPMTSWGLLLKQAQSLSELEAHPWLMIPGI
                     FIIIAVLSFNFVGDALRDAVDPYRAVEKV"
     gene            complement(3240..4208)
                     /locus_tag="PLD15_09005"
     CDS             complement(3240..4208)
                     /locus_tag="PLD15_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64087.1"
                     /translation="MWSFIVRRLLIMIPMMFFISVICFVITELQPGDFVSQYLDNPRI
                     SPAQIELLRKNLGLDEPAYVRYWNWIKGIVTRGDFGYSFAFQRPVGELIWERMGWTIF
                     VALGAIVFQWLLAVPMGIYSALHPYSPGDYTLTVVGFIGLSIPDFFMALILMYLMLKM
                     GATSIGGLFSPQFVGAPFSLAKLVDLLKHLWVPLVVVGMSGLAGLMRVMRGNMLDVVS
                     SPFITSLRAHGLDEKTVKRHAIKNALNPMVSIAGTELPNVFSGTIITAIVLNLPTMGP
                     FFYNALLNHDQYLVMSFLMFIAIITQIGNLLADIALAVLDPRIRMS"
     gene            complement(4268..>5012)
                     /locus_tag="PLD15_09010"
     CDS             complement(4268..>5012)
                     /locus_tag="PLD15_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64088.1"
                     /translation="YALDRERIIEEAYNGLAVLGGVPVLPSNKAFYNPKIEEIRRPFD
                     LAKAAEILDSLGLKDVNGDGLREFPDGSKFEFVLTVQNSPQDYQDIAYIFSRDLLDIG
                     VKVNLQILDSSLTGQMFGAGSFEVGIRAFGNQPDPQLRKAIWQPGTQLYYWHRTTRDP
                     ETNTAIFDNMLEWEKRVYDLFEKGQIAMDPVIRKTFYDEWQEIYAEYLPVIFVCKGMN
                     LYAANKALGNVEQNAEGLLSYASWTVFKK"
BASE COUNT         1033 a         1366 c         1372 g         1241 t
ORIGIN      
        1 cgttctccgc acgcgacgta aagtgcgaag agcggtttat cgttacgacg ctgcgcgtcc
       61 aggttcctcg cgaaaattgg gtacaacaga cgtgaggtga atcgtcagtc cccattttcg
      121 ggttcctggt taaggagcgc aagatgagcg acccacaacg caccacgcac cacccaccac
      181 ggctcttaga ggatgttatt tcgtgtgtag ccagcaggcc gtttcgtgtc cgggagctat
      241 ctccaccacc ggcggttttt ctttgtcgca tctatccatt ctgtaaggac acctggggtg
      301 aaaggcgcaa ccgggcggag ggttgatcgg actgggcggc tctccctcca gccttatgtt
      361 ctgtaatttt ctgtccagcg ggtcccagcc gggtatggag gccatcaagg cgccggtgta
      421 aggatgggcc gggttatcga agagatctgt ggcgccggcc gattcgacca cgttgccgag
      481 gtacatgacg agtatcctgt cgctcatatg atggacgaca tccaggttgt gagagatgaa
      541 gatgtaggag atatcgttct cggccttcag atcctgcaac aggttgatta tctggctctg
      601 gacggacaca tcgagcgcgg aggtcggttc gtcgcagatg accagcctcg gtttgacgga
      661 gatggatctc gccacggcga ttctctgtct ctggccgccg gagaattcat gcgggtacct
      721 ctgcgaatat tcggggtaga gtccaacttt catcaacagc tcggcgacgt atccgtctat
      781 ctcgttcttc ggcacgaggt tgtgggcctc tatcggttcc ctgatcacgt ccctgatggt
      841 cattctagga tcgagagagt cgaaggggtt ctggaatatc atctgtatat cccgccggaa
      901 cttcaatctg atctttttgt ccagtccctt cgttatatcg tgctctcctg cgtcattgtc
      961 gtagtaggtt atcctgccgc ccgtcggttc gtagatcttg actatcgttc tgcccagcgt
     1021 gcttttgccg cagccggatt caccgacgac tccgacggtt tcgccgcggt ttatgtggaa
     1081 gttaacgctt tccatggcct taacgtgtcc cacgacctgc aggaagactc cggccttgat
     1141 cgggaagtat ttcttgagct ctttagtctc gattatcttc atataccacc ctccccgtag
     1201 agccagcagg cggccgtgtg gttttcactc actaacatct cgggcggatc tttcttgcat
     1261 atttccatcg cgtggggaca cctcgggttg aagcggcacc cttcggggaa ctcgtaggct
     1321 ctgggaacgt agccctctat gtaaggcagt ttctggcctt tgaagtctct cttcactctg
     1381 gaattgagca atcccttcgt gtacgggtgg gccggttttt tgtaaagttc gttgacaccg
     1441 gccttttcga cgatctttcc ggcgtacatc acatggaccc tgtcggctat ctcggaaacg
     1501 actcccagat cgtgcgtgat gaatatgatc gagccgttga actcgctctg gaggtctctc
     1561 atgatgttga gcacctgcgc ctgaatggtc acgtccagcg cggtcgtagg ttcgtcggcg
     1621 attatgattt ccgggcccgt gatcagggcc atggcgatca ttatcctctg caacatcccg
     1681 ccgctcattt cgtgcgggta ctctctgtac ctcttttccg gttcgggaat gttcaccttc
     1741 cgcagcaccg agattatctc ttcacgggtt ctggcgtcgt cccacggcat gtgcgccttc
     1801 gcgacttcgg aaagctggta gcctatggtg tagagcggat cgaaggtcga catgggttcc
     1861 tgaaagatca tggagatctc tttgcctctt atgtttttgt actctttgtt cgagaggccg
     1921 actaggtttt tctccctgta aagtatctgg ccttccaccc gtgccggcgg aacttttatg
     1981 agccccatga cggtctggac agtcacgctc ttgccgcagc ccgattcgcc gaccagcgcc
     2041 agtatctcgc ccttttgaag ctcgaagctt atatcgtcca cggccttcac taaaccgtcg
     2101 ggggttctga agtaggtttt caggtttttg acttcgagta ttccgctcat accttttcca
     2161 ccgccctgta cggatcaacg gcgtctctga gcgcgtcgcc gacgaaattg aaggacagca
     2221 ccgcgattat tatgaagata ccgggtatca tgagccacgg atgggcctca agttcggaaa
     2281 gagactgggc ctgtttaagc agcagtcccc agctggtcat aggctccttt atgcccagcc
     2341 cgagaaaact tatagcgctc tctcccagga tcatgccggg aatcgatagc gtcgatacga
     2401 cgatcagata gctgatggtg ttgggtatga ggtgacgggt gattatcttc atgttggaga
     2461 cgcccgacac tctggcggcc aggacgaatt ccttttccct caggctgagc accattcccc
     2521 tttcaactct ggccactccc atccagccta tcagggaaag aacgatcact attccgaaat
     2581 agacccacgt gctcggccag ctgggcggga ggatcgtcgc gagggcaagc cagaggggga
     2641 ttcgcgggaa ggatctcagc aattcgatga acctctggat cagaacgtcc accaatcctc
     2701 cgtaatagcc ggacagcgcc ccgacgatgg cgcctatgaa gacgcttatg agcgttccga
     2761 ccagtccgac ggtcatagag actttcccgc cgatcaagac acgcgaaaag aggtcgcgtc
     2821 cgaacctgtc ggcgccgaag agcagcacca tcgccttatc ggaggattcc tcgaccccga
     2881 agaggtggat gtcggtcttg aagatgcccc agagtttgta ctcctctccc ctgacgaaga
     2941 gctttatggg tacgatcttg cttttgtcct cggagaattc gagcctgtat gttaccgggt
     3001 ttctgacctt caccatagga tggacgaagg ggccgtgcca tttgccgtct tcgtcgaaga
     3061 accttatctt cgagggggga gcgtaaacga agtttttgtg ggacgtgacg tagttgtacg
     3121 gagagatgaa atcggatagt atcaccagca ggtacatgat acccagcgcg accagaccga
     3181 tcacgcccag tttatgcttg aagaagttgc gtacgattct cttctttgta ctctgcatct
     3241 cagctcatcc ttatcctggg gtcgagaacc gccagcgcga tatccgcgag caggtttccg
     3301 atctgggtta tgatggcgat gaacatcaaa aagctcatga ccaggtactg gtcgtggttc
     3361 agaagtgcgt tatagaaaaa cggtcccatc gtaggcagat taagaactat cgcggttatt
     3421 atggttccgc tgaacacgtt cgggagctcc gttccggcga tgctcaccat ggggttcagc
     3481 gcatttttta tggcgtgtct cttgacggtc ttctcgtcca gcccgtgcgc cctcagggac
     3541 gtgatgaaag gcgaggagac cacgtcgagc atgtttcccc tcatgactct catcaggccg
     3601 gccagtccgc tcatgcccac caccacgagg gggacccaca ggtgcttgag tagatctacg
     3661 agtttggcaa gactgaaggg cgcgcccacg aactgcggcg agaagagccc gccgatggag
     3721 gtggcgccca ttttcagcat caggtacatc agtattagag ccatgaagaa gtccggtata
     3781 gagagtccga tgaaaccgac gacggtcagg gtataatcgc ccggggagta agggtgcagg
     3841 gccgaataga ttcccatggg gacggccaga agccactgga agacgatcgc tcccagagcg
     3901 acgaagatgg tccagcccat cctctcccat atcagttcgc ccaccggtct ttgaaaggcg
     3961 aaggaatagc cgaaatctcc cctggttacg atccccttga tccagttcca gtatctcaca
     4021 taggccggtt cgtccagccc caggttcttt ctgagcaact cgatctgtgc cggcgagatt
     4081 ctggggttgt ctagatactg gctgacgaaa tctcccggct gaagctccgt tatcacgaaa
     4141 catatgaccg atataaaaaa catcatcggt atcattatga gtaagcgcct cactatgaaa
     4201 gaccacatag ccttcctccg tcaaagaaaa cgcgggagga gcgccctcct cccgcagtgt
     4261 ttacaagtta cttcttgaat accgtccagg acgcgtaaga gagaagtccc tccgcgttct
     4321 gctcgacgtt tccaagcgct ttgttcgccg cgtaaaggtt catgcccttg cagacgaaga
     4381 taacgggcag gtactcggcg tagatctcct gccactcgtc gtagaaggtc tttcttatga
     4441 ccggatccat ggcgatctgt cccttttcga agaggtcgta aactctcttt tcccattcca
     4501 gcatgttatc gaagatggcc gtgttcgttt cggggtctct ggtggttctg tgccagtaat
     4561 aaagctgggt gccgggctgc cagatggcct ttctgagctg tgggtcgggc tggtttccaa
     4621 aggctcttat accaacctcg aaggagcctg cgccgaacat ctggccagtg agagagctat
     4681 cgagtatctg aaggttaacc ttcacgccta tgtcgaggag gtcccgggag aagatgtagg
     4741 caatgtcctg ataatcctgc ggagagttct gaaccgtgag tacgaattcg aacttgctcc
     4801 cgtcggggaa ttctctcaat ccgtcgccgt tgacgtcctt caagccgagg ctgtcgagta
     4861 tctccgccgc cttcgccaga tcgaacggtc tgcggatctc ttcgatcttc gggttgtaga
     4921 aggctttgtt ggagggcagt acgggaacgc caccgagaac tgccagtccg ttgtaagcct
     4981 cctcgattat cctctctctg tcgagagcgt ac
//
LOCUS       DAOOLY010000185         4999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20227_length_4999_cov_5.282160, whole genome shotgun sequence.
ACCESSION   DAOOLY010000185 DAOOLY010000000
VERSION     DAOOLY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20227_length_4999_cov_5.282160"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLD15_09015"
     CDS             <1..263
                     /locus_tag="PLD15_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB64089.1"
                     /translation="MFLGERRGTARADVFAFIEAGAGPELYDVLQFSKSASVGTPMPN
                     VPEMASVWGAMGDALGLIINGQDTVENALSAAVEKIKTTIGN"
     gene            352..2094
                     /locus_tag="PLD15_09020"
     CDS             352..2094
                     /locus_tag="PLD15_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB64090.1"
                     /translation="MSGRRFAAFLVFLAILAIFNGFALWSIYLLYADGNFGLMVTMVI
                     LTLLIDLIALNPKGYPYRYMIPAMVLLFILTLYPMYYTFRTAFTNYGTGHLFTRQQSI
                     QKLLSDYFYIPEKPQEYEFSIYIQLKDYNPTDNFITLLQSKESGELFAAPRPQAINRD
                     ATGNITLATAKMLEIKGDTFSIGGVNYSLSRSPTGSVLAIRSDTGERFVYFYSPQDQS
                     TRPNASFYFSEIRGVWLRNAEFTDSEGNQVRLFPNSLYTTFATTERKYALRAETVFSA
                     GRAIQETVVYNRQTGRKLVEEGGFFYDVDENGNDITVEGYISDVGFWNFVRMFRDPKI
                     SKPFFQVFGWTFTWAGLSVFFSFTIGLALAMTLNDRRLKGKKIYRTLMIIPWAVPAFI
                     SALVWRNGFFNETYGVLNRFIVQGLFGLEPIKWLNDAFWAKVSVLIVNTWLGFPYMMT
                     VSLGALQSIPEEFYEASSIDGATRLQRFKKITFPLLMVTVAPLLVGSFAFNFNNFTGI
                     YLVTQGGPAIVGASTPAGATDILISYTFKLAFEGRGQDFGFASAISIIIFIIVAGFSW
                     INFKLSGSFEEVSR"
     gene            2094..4865
                     /locus_tag="PLD15_09025"
     CDS             2094..4865
                     /locus_tag="PLD15_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB64091.1"
                     /translation="MAVERRIYWLRHLVLIAVVVIVLFPMVWLVSTSIRRDQAAFSSN
                     LFSSRVTLQHYRNLLLPERSVGRLILDLQSATYATGDYRGKSQEELKKNVESYLAKFD
                     SLMEESQRMVSNIESGMAEIEKDVVSKEKQILLEMNEIRIKDKELFENRLKQIGTFEE
                     PQMAYAVGRILETTVPSLEGVYPFYTDLLGERAAPITEAMEGYRQMYESTLKYRDETL
                     KALVGLEFENREEVITSLNSIDKYISLSSVDYSEWRRVEWLRVINRYLREVESSLPEM
                     EASVLNQARTNLYESFRAAGDSWEAVLTSNGEVSQELSSLAGEAFGSGYYKYIEASEV
                     LSGMENSIAELKKTLAVSESAVLELEDSFEIAIPTVISETRKLSALIPPFELVASKGI
                     ESSDPVSGAKLTGSLDGVLSARETINLVNGQLAGMKNVEELSSELSDLSSKLSWFVDN
                     REVLMKASGNSEISKGADVINIALSNLSRVLPVLQTSVSEYLEVSKSAEESKSRLQTL
                     EGEKEKLLDTISNHEEEARKAEESYVHALEAINIRSAMLYTDREILSIEEARNYVLNL
                     TNVLNSYFNIKAPVRYRTLVWYDDFAKADTEARDGTVLLKELISSMRVMKDDLASKIE
                     NYIELRFLGTSITLDDFGKMQETYNSLFQQVNAKYQRASRLISDLIEKPTSYSESYAA
                     ELREADKALFRANQIWVQKESTYFYFVRWLLNSVIVALAVALISVTVAALAAYPFSRM
                     RFPGRKQGLLALLLVQMFPSIMFMVALYALLQFLGSVVPFLGLNTLGGLIFVYSGGIA
                     FNIWLIKGYYDTISNSLEEAAMIDGATKFQAFTKVILPLARPILAVVAILTFMNIFNE
                     YLIARIILQDMNKWTYAVGLWQFSGRFETSWGPFTAAALIGAVPMVIFFLVLQDYIVG
                     GLTQGGVKE"
BASE COUNT         1279 a         1185 c         1269 g         1266 t
ORIGIN      
        1 gtatgttcct tggtgagaga aggggaacgg ccagagccga cgtatttgcg ttcattgaag
       61 cgggcgccgg tccggagctt tacgatgttc ttcagttctc caagagcgct tccgtcggta
      121 ccccgatgcc gaacgttccg gaaatggcaa gtgtctgggg agccatgggt gacgcgcttg
      181 gcctcataat caacggccag gacaccgtgg aaaacgcgtt gagcgcagca gttgaaaaga
      241 tcaagaccac tataggcaat tgattctatt cttatcacat ctctaaacat ggttaggctg
      301 agcgcggggc tattccccgc gctctggttc taattgcctg gagggattac cttgagtgga
      361 agacgttttg ccgcatttct cgtttttctg gctattttag ccattttcaa cggtttcgcg
      421 ttgtggagta tctatctact ctatgcagac ggaaatttcg gcctgatggt taccatggtg
      481 attcttacgc ttctcataga tttgatcgcg cttaatccta aaggataccc gtacagatac
      541 atgatcccag ctatggttct ccttttcatt ctcactctat accccatgta ttacacgttc
      601 agaactgctt tcaccaatta cggcaccggg catctcttca ccaggcaaca gtctattcaa
      661 aaattgttga gcgactactt ctatattcct gaaaaaccgc aagagtacga gttttccata
      721 tatatacagc tcaaagacta caacccgacc gataacttca taactctact ccagtcgaaa
      781 gaaagcggag agctttttgc cgccccgaga ccgcaggcga taaacaggga tgcgactgga
      841 aatataactc ttgccactgc caaaatgctt gaaatcaagg gtgatacatt ttcgatcggc
      901 ggcgtcaact attcactatc ccgctcgccg acggggagtg tcctggctat aaggtcggat
      961 acaggggagc gtttcgtcta tttctactcg ccacaggacc aatcgacaag accgaacgca
     1021 tccttttatt tcagcgaaat aaggggtgtc tggcttcgca acgccgagtt caccgatagc
     1081 gaaggcaacc aggtaagact ctttccaaac tccctttaca ccacttttgc gacaacggaa
     1141 agaaaatacg ctttgagagc cgaaacggtt ttctcagccg gcagggctat tcaagaaacg
     1201 gttgtgtaca acagacagac gggaagaaag ctcgttgaag agggcggctt cttttacgat
     1261 gtcgatgaaa atggaaacga tatcactgta gaaggttata tatcggatgt cgggttctgg
     1321 aacttcgtca ggatgttccg tgacccgaag atcagcaagc ccttctttca ggtgttcggc
     1381 tggactttca cctgggccgg gctcagtgtt ttcttctctt tcacaatcgg tctggctctt
     1441 gcaatgacgc tcaacgaccg caggttgaaa gggaagaaaa tctaccgaac cctcatgatc
     1501 ataccgtggg cggttccggc gttcatatcg gcccttgtgt ggagaaacgg tttcttcaac
     1561 gagacttacg gtgttctcaa cagattcata gtacaggggc ttttcggtct ggaaccgatt
     1621 aaatggttaa acgacgcttt ctgggcaaaa gtatccgttc tcatcgtaaa tacctggctg
     1681 ggtttcccgt acatgatgac cgtcagcctt ggggcgcttc aaagtattcc cgaggagttt
     1741 tacgaagcat cttcgatcga tggcgcaacc cggttgcaga gatttaaaaa gatcaccttt
     1801 cctctgctga tggttacggt ggctcctctg ctggtcggga gtttcgcctt caacttcaac
     1861 aacttcacgg ggatatatct ggtgacccag ggaggtccgg cgatcgtggg cgcatccacg
     1921 ccggccggag cgacggatat actgatctct tacactttca agctggcctt tgaggggaga
     1981 ggtcaggact tcggctttgc cagcgccatt tcgatcatca ttttcataat cgttgccgga
     2041 ttcagctgga ttaacttcaa gctttccggt tcctttgaag aggtgagcag ataatggccg
     2101 tcgagagaag aatttactgg ttgagacatc tagtacttat agccgttgtc gtgatagttc
     2161 tctttccgat ggtatggctg gtgagcacat ctataaggcg cgatcaggcg gccttttcat
     2221 cgaacctctt ttccagtaga gtgactctcc agcattacag gaacctgcta ttacccgaga
     2281 gaagcgtcgg aagattgatt ttggatttac agagtgccac ctacgctaca ggagattacc
     2341 ggggaaagag tcaggaagag ctcaaaaaaa acgttgagag ttacctggcc aaattcgatt
     2401 ctctcatgga agaaagccag agaatggtgt cgaacatcga atcgggtatg gcggaaatag
     2461 agaaagacgt cgtaagcaaa gagaagcaga tactcctcga gatgaacgag ataagaatta
     2521 aagacaaaga gcttttcgaa aaccgtttga aacagatcgg tacttttgaa gagccccaga
     2581 tggcctatgc tgtcggcagg attctcgaga ccaccgttcc atctctggaa ggagtttatc
     2641 ccttctacac cgatcttctc ggagaacggg cagctccgat aactgaagct atggaaggct
     2701 acaggcagat gtacgagagt acactgaaat accgcgatga gactctaaag gcgctcgtcg
     2761 ggttagagtt tgagaaccgt gaagaagtga taacatcact gaattcgata gacaaataca
     2821 tctctctttc ttccgtggac tactccgagt ggaggagggt ggaatggtta agggtaatca
     2881 acaggtattt gcgtgaagtg gagagttctc ttccggaaat ggaagcctct gttcttaatc
     2941 aggcgagaac aaacctttac gaatctttca gggcggccgg agattcctgg gaagcggttc
     3001 taacatccaa cggggaagtt tcacaggagc tctcatccct tgccggtgaa gctttcggtt
     3061 ccggttatta taaatacatc gaagcaagcg aggttctttc aggcatggaa aactccatag
     3121 cggaactcaa aaaaaccctt gccgtttcag aatctgccgt tctggaactc gaagactcgt
     3181 tcgagatagc tattccaact gtgatatccg aaaccaggaa actgtcggcc ttgattcccc
     3241 catttgagct agtggcctca aaaggtatcg aaagcagcga cccggtctca ggcgccaaac
     3301 tgacaggctc gctagatggg gttctgtcgg ccagggaaac cataaatcta gtgaacggtc
     3361 agctggcagg tatgaagaat gttgaggaac tctcttcaga gctttccgat ctttcatcga
     3421 aactctcctg gttcgtcgat aacagagaag tgctcatgaa agcctcgggc aactctgaga
     3481 tctccaaggg agccgatgtg atcaatatag ccctgtcgaa tctttcgaga gtcttgccgg
     3541 ttctccagac cagtgtttcc gagtatcttg aggtttcgaa gagtgccgaa gagtctaaat
     3601 ccaggctgca gactctggaa ggcgagaagg aaaaactgct agatacgatt tcaaatcacg
     3661 aagaagaggc gagaaaggcc gaagagtctt acgtgcatgc actggaagct attaacataa
     3721 ggtcggcgat gctttacacg gaccgggaga tcctctcgat agaagaggcc agaaattacg
     3781 ttttgaattt aacgaacgtt ctgaacagct atttcaatat caaagccccc gtccgctacc
     3841 ggacgctggt atggtacgac gacttcgcca aggccgacac cgaggcaaga gatggcaccg
     3901 ttctgttgaa agagttgata tcttcgatga gagtgatgaa ggacgatctc gcttcgaaga
     3961 tagagaacta tatagaactc aggtttctgg gaacatcgat aacgctcgac gatttcggga
     4021 agatgcagga aacgtacaac tctctcttcc agcaggtcaa cgccaagtat caaagggcgt
     4081 ccagactgat ttcagacctt atagaaaaac ccacttctta ttccgaatcg tacgcggccg
     4141 aactcagaga agcggataaa gcccttttca gggcgaacca gatctgggtt cagaaagaga
     4201 gcacgtactt ctattttgtc agatggcttc tgaactccgt catagtggcg ctggccgttg
     4261 cactcataag tgttacggtc gctgctctgg ccgcgtaccc attcagtaga atgagattcc
     4321 caggcaggaa acagggactt ctggcactgc ttctggttca aatgtttcca tcgatcatgt
     4381 tcatggttgc cctgtacgcg ttgcttcaat tcctgggaag cgttgttccc ttcctggggc
     4441 tcaacactct cggaggtttg atattcgtgt actccggggg gatcgcattc aacatatggc
     4501 tcataaaggg ttattacgac acgatatcta attcgctgga agaggctgcc atgatcgatg
     4561 gagcaacgaa attccaggcc ttcaccaagg tcattctacc ccttgccaga cccattctgg
     4621 cggtggttgc gatactgacc ttcatgaata ttttcaacga atatctcatc gcaagaataa
     4681 tccttcagga tatgaacaaa tggacgtatg ccgtcggcct ctggcagttt tccggtaggt
     4741 ttgaaaccag ctgggggcca ttcacagcgg ccgccttgat aggtgcggtt ccgatggtca
     4801 ttttcttcct ggttttgcag gattacatag tcggaggcct cacacagggt ggcgtgaaag
     4861 agtaattcca acgacggggc ggatctctcc gccccgcttt tatggaggtt ttatggaact
     4921 cgtctttccc gactacgaag gaagttcgat agtgaatttc tcggctggcc ttctggaaca
     4981 ctttatggcc cagcgcccc
//
LOCUS       DAOOLY010000186         4998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20234_length_4998_cov_4.702610, whole genome shotgun sequence.
ACCESSION   DAOOLY010000186 DAOOLY010000000
VERSION     DAOOLY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20234_length_4998_cov_4.702610"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1597)
                     /locus_tag="PLD15_09030"
     CDS             complement(77..1597)
                     /locus_tag="PLD15_09030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB64092.1"
                     /translation="MYLIGCDIGTQGTKSVMVDESGRVLVEAQREYDVIKPGSNWAEQ
                     WPDVWARAAFETIWEVVEKSGVAKEEIAALAISGLYGGSGVPVDREMKPLYPCLIWMD
                     RRATYQTGWVKDNIPKEEIFSITGNYVDSYYGFTKMMWLRDNRPEIWKKIYKFVTPKD
                     YVVYQLTGENAIDYSSAGNIGGVFNIRKLTWSTDMCKALGIPIEYLPDRIVRSSDVVG
                     KITRASSKLCGLPEGTPVVSGGIDAPVAQLSAGALEEGEHVAMVGTSTCWGTVHDGSE
                     LPFGLVSFPYVVHDTERIYSFGGSATTGALARWFKEEFADSETSVGLRTGISPYQLLD
                     REVETIPAGSDGIIVLPYFMGERSPIWDPFARGVFFGVTLVHTRGHMYKALMEGAAFA
                     LRHNIEEGIKAGLKLNDECWIVGGVAKSSAWNSIFADITGYKMRQVSSLVEAPFGDAF
                     LAGLGVGLIDRPEKIKEWVKFREPINPDPNNREIYEKSYALFRQLYERTKDLMWQI"
     gene            complement(1612..3036)
                     /locus_tag="PLD15_09035"
     CDS             complement(1612..3036)
                     /locus_tag="PLD15_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HPB64093.1"
                     /translation="MYQKLKLGFAPTRRNVFSKKDAQEHKMLIEKKLKAWNVDFVGLD
                     WLNEEGLIYDPKDALSVAERFRNAGVNAIFAPHVNFGTEEAVAKLSRELNLPLLLWGP
                     RDDAPLPDGSRTRDTQCGLFATGKLLRRFGVPFTYIVNSSVDDSLFERGFKNFLAAAS
                     VVRAFRNMKIGVISNRPRDFWTVINNEGELLEKFGIELIPTTLIDIVEEAKKLLDSKE
                     LDQELAGLKDRVEYSSAGEESFKKVLTLKIVMERWAIEEGLSGIAIQCWNALQKAYGV
                     MPCFAHSLLSDSKIPVACETDVHGAISMELAQAATMYRKPSFLADLTIRHPSDDNAEL
                     LWHCGPFPLSLAKEKPYVGKHFILESHSPGVSEWEIEGGKLSILRFDGDFSNYKVFVS
                     HAESTDGPFNKGTYIWAKFKNWPKLEEKLVCGPYIHHVAGVHDRIMPVIYEAVKYMKG
                     VELDPGEPGIDEVQSWLRHEIDEI"
     gene            complement(3137..4621)
                     /locus_tag="PLD15_09040"
     CDS             complement(3137..4621)
                     /locus_tag="PLD15_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPB64094.1"
                     /translation="MQTYVRVRPEKLGKINDMIYGHFTEHLGRCIYDGIFNEKSSKSD
                     SRGFRRDIIDSVKKIKCPILRWPGGNFASAYHWQDGIGPVEKRPQLLNYIWGGLESNR
                     FGTDEFIQYCCELGTEPYLAVSLGTGTLDEAVAWLEYCNLDTPTRYAGMRRENGHAEP
                     YGVKYWGIGNEVYGPWQVGHCSATEYAEKLRQYTQFMKGVDRSIKVIAVGADNPDWDM
                     TVLKHAGHLIDYISIHEYHGSDDYFGTVASVYYCEQRLKLLENLIKHLRLDHVKISFD
                     EWNVWYKISAEREGTEKGTVLIEEPYALKDGLFAAGIFAVMHRMNDTVQMANLAQMVN
                     ALGMIKTKGDEIVLTPIYHAFDLFVNHTGRTRLAMDIDTQRYDIKARTFVEGRNSFQL
                     KGVPYIDGSATYDERRNVLSVALINYYQKETDIRLDLSGLEAKKKADRYVLTGSAPDE
                     MNDFDNPDRIHLVNDELAVGTREMNLRLAPMSASVIEFTLEKTL"
     gene            complement(4660..>4998)
                     /locus_tag="PLD15_09045"
     CDS             complement(4660..>4998)
                     /locus_tag="PLD15_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPB64095.1"
                     /translation="LTDEDVEVIGYWFKSLYHSCSFDYETGDLIIKVVNPWPEDKEVL
                     VEIDEATKVTGKGEATVITSDSIFDGNTFEDPERIVPMTTVLSDFSNRFTFSFEGNSI
                     TVLRLNTGSL"
BASE COUNT         1054 a         1414 c         1185 g         1345 t
ORIGIN      
        1 agtaaactat tcagatcttc gctatcgcct ttccctctga cagccacaat gaaaaggtgg
       61 ggtctccccc accgtatcag atttgccaca tcaagtcttt cgttctctcg taaagctgac
      121 ggaagagtgc gtaagatttt tcgtatatct cccgattgtt cggatcggga ttgatcggtt
      181 ctctgaactt cacccactct tttatcttct caggtctgtc gatcaatccc acgcccagcc
      241 ctgccaagaa ggcatctccg aagggagcct ccacaaggct ggatacctgt ctcatcttgt
      301 agccggtgat atcggcgaag atgctgttcc aggcagagga tttagccact ccgccgacta
      361 tccagcattc atcgttcagt ttcaggccgg ccttgattcc ctcctcgatg ttatgtcgca
      421 gggcgaatgc cgctccttcc atgagggctt tgtacatgtg gcccctcgtg tgcacgaggg
      481 tcactccgaa aaaaacccct ctggcaaagg gatcccagat cggcgatctt tcgcccatga
      541 agtaagggag tactattatt ccgtcgcttc cggccggtat cgtctcgact tccctgtcaa
      601 gaagctgata cggtgaaata cccgttcgca aacccacaga cgtttcgctg tccgcgaatt
      661 cctctttgaa ccatctggcg agcgctccgg tagtggcaga accaccgaag gagtatatcc
      721 gctcggtatc gtgaaccaca tacggaaaac tgacgagccc gaagggtagc tcgctgccgt
      781 cgtggacggt tccccagcag gtagaggtgc cgaccatggc gacgtgttcg ccctcttcga
      841 gcgccccggc cgaaagctgg gcgacggggg cgtcgattcc accggaaacg acgggcgttc
      901 cctcgggcag gccgcagagt tttgaactgg cccgagtgat ctttccaacc acatcgctag
      961 atctcacgat tctatcggga aggtattcga tcgggattcc cagagcttta cacatatctg
     1021 tagaccatgt aagttttctt atgttgaaaa caccgcctat attccctgcc gatgagtagt
     1081 cgatggcatt ttctccggta agctgataga caacgtaatc tttcggcgtc acgaacttgt
     1141 atatcttctt ccagatctcg ggtctgttgt ccctgagcca catcattttg gtgaagccat
     1201 agtaggaatc gacgtaattg ccggttattg aaaagatctc ttctttgggt atgttgtcct
     1261 taacccagcc agtttgatac gtcgcacgcc tgtccatcca tattagacag gggtagagcg
     1321 gtttcatctc tctatccacc ggtacaccgg agccgccgta gagaccgctg atcgccagag
     1381 cggcgatctc ttccttcgcc actcccgatt tttctaccac ttcccatatc gtttcgaagg
     1441 cagccctcgc ccagacgtcc ggccactgtt cggcccagtt cgagccgggt ttgatgacat
     1501 cgtactctct ctgcgcctcg acgagaacac gtccgctttc gtccaccata acgcttttcg
     1561 taccctgagt cccaatatcg cacccgatca aatacatatc cataccacct tttagatttc
     1621 gtctatttca tgtctcaacc aagattgtac ctcatctatt ccaggctcgc caggatcgag
     1681 ctctacgccc ttcatgtatt tgacggcctc atagatcaca ggcattatcc tgtcgtgaac
     1741 accggccacg tggtgaatgt agggaccgca taccagtttc tcttcgagct ttggccagtt
     1801 cttgaatttc gcccagatgt acgttccctt gttgaaaggt ccgtccgtgc tttcggcgtg
     1861 agatacgaaa accttgtaat tcgagaagtc tccgtcgaat ctgagaatgc tgagcttacc
     1921 cccctctatc tcccattccg atacccctgg actgtggctc tcgagtatga agtgtttccc
     1981 gacgtatggt ttttccttcg cgagcgaaag cgggaaaggc ccgcaatgcc acagaagctc
     2041 ggcattgtcg tcggacggat gtctgatcgt cagatcggcc agaaaggagg gttttcgata
     2101 catggtggcc gcctgggcca gttccatgct gatcgccccg tgaacgtcgg tttcacaggc
     2161 aacggggatt ttactgtccg aaagcagaga atgggcaaaa cacggcataa cgccataagc
     2221 cttctgtaga gcgttccagc attgaattgc tattccgctc aagccctctt cgatcgccca
     2281 cctctccatc acgatcttga gcgtcaggac ctttttgaaa ctctcttcgc ccgcgctgga
     2341 gtattcaacc ctgtccttga gtccggccag ttcctggtcg agctctttcg agtccagtaa
     2401 ttttttcgcc tcttccacta tatctatcag ggtagtaggg atgagctcta taccgaactt
     2461 ctccagaagc tcgccttcgt tgtttataac ggtccagaag tctctcggcc tgttgcttat
     2521 gactccgatt ttcatattgc ggaaggccct gaccaccgaa gcagcggcga gaaagttttt
     2581 aaatccccgt tcgaagaggg aatcatcgac cgaggagttc actatatagg tgaagggaac
     2641 gccgaagcgt ctcaacaact tacccgtcgc gaagagaccg cactgggtgt cacgcgttcg
     2701 actgccgtca ggcagcggag catcgtctcg cggcccccag aggagcaggg gcaagttgag
     2761 ttctctggag agcttggcaa cggcttcttc ggttccgaaa ttcacatgag gcgcaaagat
     2821 ggcgttcact ccggcgtttc tgaaacgttc cgccactgag agcgcgtctt tgggatcgta
     2881 aatgagcccc tcttcgttca gccagtcgag accgacgaaa tctacattcc aggccttcag
     2941 ctttttttcg atgagcattt tgtgttcctg agcgtctttc ttgctgaaaa cgtttctcct
     3001 tgtgggagcg aacccgagtt ttagcttttg atacatcctt tccctccttt caacctcccg
     3061 gatgactcag tcggccggac ttggaagaat acgcccgtcg tagtttttct gccgttagct
     3121 tgtcgatttt tcaaactcaa agtgtttttt ccagagtgaa ctcgattaca ctggccgaca
     3181 tgggagcgag cctgagattc atctccctgg ttcccacggc cagttcatcg ttcacaagat
     3241 gtatcctatc ggggttgtcg aagtcgttca tctcgtcagg agccgagccg gtcagaacgt
     3301 accgatcggc ctttttctta gcttccagcc ccgaaagatc gagacgtata tcggtttcct
     3361 tctgataata gttgatcagc gctacgctga gtacgtttct cctttcgtcg taagtggccg
     3421 aaccgtcgat gtatggaaca cccttcagct ggaagctgtt gcgcccctcg acgaacgttc
     3481 tggcctttat gtcatacctt tgtgtgtcga tatccatggc gagtctggtc cggcccgtgt
     3541 ggttcacgaa gagatcgaag gcgtgataga tcggagtaag gacgatctcg tctcccttcg
     3601 tctttatcat acccagggcg ttaaccatct gcgccagatt ggccatctgt accgtatcgt
     3661 tcattctgtg catcacggcg aatattccgg ccgcgaagag cccatccttc agtgcgtacg
     3721 gttcttcgat gagtaccgtt cccttctctg tgccctccct ttccgcactg attttgtacc
     3781 agacattcca ttcgtcgaac gagatcttca catgatccag gcgaaggtgc tttataaggt
     3841 tctcgagcag cttcaatctc tgctcgcagt aatacaccga agccacggtg ccgaagtaat
     3901 cgtccgaacc gtggtactca tgaatcgaga tataatcgat caggtgcccg gcgtgtttca
     3961 acaccgtcat atcccagtcg ggattgtcgg cgccaacggc tataaccttt atcgaacggt
     4021 ccacgccctt catgaactgt gtgtactgcc tcagcttttc ggcgtattcg gtcgcgctgc
     4081 agtgaccgac ctgccagggg ccgtacactt cgtttccaat cccccagtac ttaactccat
     4141 acggctcggc atggccattt tctctcctca ttcctgcgta tctggtcggc gtatcgaggt
     4201 tacagtattc gagccaggcg acagcctcgt cgagcgttcc cgtccccaga ctcacggcca
     4261 gatagggttc ggtgccaagc tcgcaacagt actgtatgaa ttcatccgtt ccgaacctgt
     4321 tgctttccaa cccgccccat atatagttca gcagctgcgg ccgtttctcc accggtccga
     4381 taccgtcctg ccagtggtac gcggacgcga agttgccacc aggccatctc aagatggggc
     4441 acttgatctt ttttacggaa tctatgatat ctctcctgaa tcctcgcgag tcggactttg
     4501 aggatttttc attgaagatt ccgtcgtaga tacatctacc cagatgctcc gtgaaatgtc
     4561 catagatcat atcgtttatc tttccgagtt tttccggtct gaccctgacg tatgtctgca
     4621 ctgtaatccc tccctgattg actaatcgtt atgagatttt taaagactcc cggtattcaa
     4681 tctcaagacc gtgatcgaat taccttcgaa gctgaatgtg aatcgattgg agaaatccga
     4741 caaaaccgtg gtcatgggta cgatcctttc gggatcttcg aaagtgtttc cgtcgaagat
     4801 actatcggaa gttatcactg tcgcttcccc tttgcccgtc acttttgtgg cttcatctat
     4861 ttccaccaga acctctttat cctcgggcca ggggttgacg acctttatta tcaaatctcc
     4921 ggtttcgtag tcaaagctac aggaatgata aagcgacttg aaccaatagc ctatgacttc
     4981 gacatcttcg tccgtaag
//
LOCUS       DAOOLY010000187         4992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20274_length_4992_cov_4.390521, whole genome shotgun sequence.
ACCESSION   DAOOLY010000187 DAOOLY010000000
VERSION     DAOOLY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20274_length_4992_cov_4.390521"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..633)
                     /locus_tag="PLD15_09050"
     CDS             complement(70..633)
                     /locus_tag="PLD15_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB64096.1"
                     /translation="MDEKREEAINNMDQNETPFGVQENSFETESASKVEIDELKASVK
                     DLIEENARLRAEFQNFKTAIRREAEEQVRRSKERTVLRIIDIYMDLNRALANSQNRDD
                     GLFLGIELISKSMEKLMSDEGVSFIEPEIGMPFDPFANEVDETVSTNEVPDMAVYKVN
                     AIGYNFNGKVLRPARVVVALNSSPTDE"
     gene            complement(652..1695)
                     /gene="hrcA"
                     /locus_tag="PLD15_09055"
     CDS             complement(652..1695)
                     /gene="hrcA"
                     /locus_tag="PLD15_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPB64097.1"
                     /translation="MSRRRMIGPDLNSRQVRVLYCISREYISTGKPVSSKQVLEHSNL
                     NFSSATVRNDMRKLEFLGYINQPHTSAGRVLTDKGLRFYLDSIKSISRDLQESKVAIA
                     IRQASFMGDTERLLQGMTRILAHAASAFVIIEKPKLDRLVVRSISISRIAESYLNVSV
                     ITDLGVSLNTTVFTGFSEFDVDTFQRQINMAVVGKTIGEIRKGIRNVEIKEDQWYDRR
                     VQEILHFLQSIFDKENEEKYFRYGLEFVIVSEYLDSQDVTDLVRAIENPKNLEGLLKE
                     LGEVDSPKAFIGQEIGREEMKNYSIFASPYTRNDEKIGTVYIVTPKLSYYEKITGYLE
                     FSINRLSEVFSNR"
     gene            1803..3299
                     /locus_tag="PLD15_09060"
     CDS             1803..3299
                     /locus_tag="PLD15_09060"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPB64098.1"
                     /translation="MRLSQIIELLGNNVLQVVNPGNVDPEFEHVESDSRILKENVLFI
                     CIKGQAFDSHLIAKELQKKGAVALIAETVIEDEELFIPIVYVKSTRLVEALLTMEEYN
                     HPYRKLTTIGVTGTNGKTTITTLIYHVLSIFERKASLIGTVRNVVGENVYTNPKNTTP
                     GPFELARLLQLSNKLKSEYFVMEVSSHSLSMNRVEGMRFDVGIITNVTRDHLDFHFSF
                     EDYYRAKMHLFSLLKSNGIAVVNGDKINIADIHISRSRIITYGFGDESDYRIEDLDVS
                     RTGMDFTIHTPFGSSQRVYTRLIGEHNAYNVAAAIAALNSLNYDMDHIIKAISSFGGV
                     PGRFEFVEEATKYGFDVVIDFAHTPDALEKLLRTARRLSPGRLILVFGAGGSADKGKR
                     PLMSEVSSRFSDVVILTSDDPKNDDPLEILQDLESGIDKFKPYLVIPDRKEAISVALT
                     LANRQDMVLIAGRGHEDFQILKDRKVPFNDKQVVKDILETKFRRHIKK"
     gene            3296..4621
                     /gene="murF"
                     /locus_tag="PLD15_09065"
     CDS             3296..4621
                     /gene="murF"
                     /locus_tag="PLD15_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPB64099.1"
                     /translation="MNSNEAVEEFLKLTENRKISVDSREIKEGDVFLALKGEKIDGND
                     FVLDALNRGAASAFTSRKFNDKRAIYCQDIKDLLMRASRLLLAGAHFEKLISITGSNG
                     KTTTKEIVAFLLSKIGRTFKTEGNLNTEIGLPLSLINGRREFLFARFGVFELGTNNKG
                     DLEKLVSLLEPQVCVLLNVGSAHLGNFGSPEDLLKEKLSIFNSRRMTTAITNGDDTRI
                     REFVRNLKCEKYFFGTGRYDFTLVDFSYDDRSTKVHFVSDGDKFVRLNGLWSAGQIMD
                     FGAAYLVAKGCGLEEPAIDIAGLSLSFNDRFKVEKIHGMTLISDFYNSSLESWRSAVD
                     SIKKIESKRKIAVVGSILEQGTHSGDTHMELGKILGAFDEVLFFNTDRDIEMACGTVK
                     PVLISDDVRELAWWLKENAKKGDLIFFKASRSVFLERVFGKFMELIKDG"
     gene            4614..>4992
                     /locus_tag="PLD15_09070"
     CDS             4614..>4992
                     /locus_tag="PLD15_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPB64100.1"
                     /translation="MVDQVLSFLIPLVATAILLEPFKRFQRKYKIGQYIRQEGPDLHN
                     YKTGTPTASGVIFIPISLIVMILFSRQPETLIVVSAGILFGAVGLIDDISKIAKKNAA
                     GLSGKRELLLQFVFASIVVLMIQL"
BASE COUNT         1246 a         1227 c         1189 g         1330 t
ORIGIN      
        1 ttgcttcggg atgccctcga acgggccgga accctcgatg gagccatgaa aatcacctgc
       61 cacaggttct cattcgtccg ttggagagct gtttagagct accactaccc ttgccggtct
      121 gagaaccttc ccgttgaaat tgtaaccgat cgcattcacc ttatacactg ccatgtccgg
      181 tacttcgttc gttgatacgg tctcgtccac ttcgttggcg aaaggatcga aaggcattcc
      241 gatctcaggc tcaatgaagg agaccccctc gtccgacatc agtttttcca tcgacttcga
      301 tatcaattct atacccagaa aaagaccgtc gtctcgattt tgactgttgg ccagcgccct
      361 attcagatcc atgtagatat ctattatcct cagaaccgtt ctctctttgc ttcttctgac
      421 ctgttcttcc gcttccctgc gtatcgccgt tttgaagttc tggaactcgg ctctcaacct
      481 tgcgttttct tctatcaggt ccttgaccga tgccttcaat tcatctatct caacttttga
      541 agcactttcc gtttcaaatg agttttcctg cactccgaaa ggagtctcgt tttgatccat
      601 gttgttgatg gcttcttcgc gcttttcgtc cataaagttt cctcctgtct gctatctgtt
      661 cgaaaaaacc tccgaaagtc tgtttataga gaattcgaga tagcccgtta tcttctcgta
      721 atacgacagc ttcggagtca ctatataaac cgtacctatc ttctcatcgt ttctggtgta
      781 aggagaagcg aatatagagt agttcttcat ctcctccctg ccaatctcct ggcctatgaa
      841 agcctttgga gaatctacct ctcccagttc ctttaggagt ccctcaaggt ttttgggatt
      901 ctctatcgcc cttacaagat ctgtgacatc ttgagagtca agatactcac tgactatgac
      961 gaactctagt ccatacctaa aatacttctc ttcgttttcc ttgtcgaata tactttgcag
     1021 gaagtgaagt atctcctgta ctctcctgtc gtaccattga tcctctttta tttcgacgtt
     1081 cctgatgccc tttcgtatct cgcctatggt ctttccgacc accgccatgt tgatttgtct
     1141 ttggaaagta tcgacgtcaa attcgctaaa gcccgtaaaa acagtggtgt tcaaactcac
     1201 gcccagatcg gtgatcaccg aaacgttcag gtaactctcg gctattctgg atatcgaaat
     1261 cgacctcacg accagtctat ctagtttcgg cttctcgatt atcacgaagg ccgaggctgc
     1321 gtgagcgaga atcctggtca tgccctgtag tagtctttcg gtatcgccca tgaagctggc
     1381 ctggcggatg gctatggcca ctttgctctc ctgcaagtcc ctggagatgc tctttattga
     1441 atcgagataa aatctcaatc ccttgtccgt cagaacccta ccggccgatg tgtgcggctg
     1501 gttgatatac cccagaaact cgagctttct catatcgttc cttaccgtcg ccgagctgaa
     1561 attgaggttt gaatgctcga gaacctgttt cgaactcacg ggcttgccgg tggatatgta
     1621 ctctctcgat atacagtaaa gaactcttac ctgtctggag tttagatccg gaccaatcat
     1681 tcgccttcta gacatttagc actccttcca aaagactgct aattaatgtt agcatacgcc
     1741 gcctcagttg tcaagttcag ggagcgatga tacaatcttc tagagcgaaa ggggtgtgat
     1801 tcatgagatt gtctcagatc atagaactcc tcggcaacaa cgtccttcag gtagtgaacc
     1861 ccggtaatgt tgaccccgaa tttgagcatg tggagagcga ttcaagaatt ctcaaagaga
     1921 atgttctctt catatgcata aagggtcagg ctttcgactc gcacctgatt gccaaagagc
     1981 tgcaaaaaaa gggtgcggtt gcgctgatag ccgagactgt cattgaagat gaagagttat
     2041 tcattcccat cgtttacgtg aaaagtaccc gtctcgtaga ggctttgttg accatggagg
     2101 agtacaacca cccctatcgt aaactaacga ctataggagt caccggtaca aacggaaaga
     2161 cgacgataac cactctcata tatcatgtgc tctctatttt tgagaggaaa gcctccctca
     2221 tcgggactgt gagaaacgtg gtcggcgaaa acgtatatac gaaccccaag aacaccacgc
     2281 ctggtccttt cgagctggcc agactccttc aactttcgaa caaattgaag tccgaatact
     2341 tcgtcatgga agtctcttca cattcgcttt ccatgaacag agtcgagggc atgaggttcg
     2401 atgttggtat cataaccaac gttacgcggg accacctgga ttttcacttc tcctttgaag
     2461 attactacag ggctaagatg cacctgtttt cgcttttgaa atccaacggt atagccgtag
     2521 tgaacggaga caaaataaac atcgcagaca ttcacatatc cagaagccgc atcataacgt
     2581 acggtttcgg agacgagtcg gattacagga tcgaagatct cgatgtttcc cgcaccggaa
     2641 tggattttac cattcacact cctttcggtt cctctcagag ggtttacacc cgcctgatag
     2701 gggagcacaa cgcgtacaac gtggcggccg ccatcgccgc gctcaattcg ttgaattacg
     2761 atatggatca tataataaag gctatttctt cttttggtgg agtgcccggg aggttcgaat
     2821 tcgtcgaaga ggccaccaag tacggtttcg atgtcgttat agacttcgcc cacacgccag
     2881 atgctctgga aaaacttctg cgcaccgcca gaaggctcag ccctgggagg ttgatcctcg
     2941 tctttggagc aggtggatcg gcagacaaag gaaagagacc tttgatgtcg gaagtttctt
     3001 cgcggttcag cgatgtggtg attctgacct ccgacgatcc gaaaaacgac gatcctctgg
     3061 agatacttca agatctggag agcggcatcg acaaattcaa gccttatctc gtgatcccgg
     3121 ataggaaaga ggccatttcc gtcgccctta cgctggccaa caggcaggat atggttttga
     3181 tagccggcag ggggcacgaa gacttccaga ttctcaagga caggaaagtg cctttcaacg
     3241 ataagcaagt cgttaaagat attttagaaa ctaaattccg gaggcacatc aagaagtgaa
     3301 ttctaatgaa gcggtcgaag agtttctgaa gctcacggaa aatcgtaaaa tctccgtcga
     3361 ttccagagaa ataaaggaag gtgacgtttt tctggccctt aagggagaga agatcgacgg
     3421 caacgatttc gtgctcgatg ccctaaacag gggggcggct tccgctttca cttcacgaaa
     3481 attcaacgat aaacgtgcca tatactgtca agatataaag gatctactga tgagggccag
     3541 caggctgcta ctggccggag cgcatttcga aaagctgatc tcgataacgg gctcgaatgg
     3601 caaaacaacg accaaagaga tagtggcctt tctcctctcg aagatcggca ggactttcaa
     3661 aaccgagggc aatctgaaca ccgaaatcgg gctgccgtta tctcttataa acggcaggag
     3721 agaatttctt tttgccagat tcggtgtctt tgagcttggc accaacaaca agggcgatct
     3781 ggaaaagctg gtatcgcttc tggagcctca ggtctgcgtt cttctcaatg tcggcagcgc
     3841 ccatctgggc aattttggga gtccggaaga ccttctcaag gaaaagctgt ccattttcaa
     3901 ttctagacgc atgaccaccg cgataacgaa cggtgacgat acgagaatca gggagttcgt
     3961 tcgaaacctc aaatgcgaga agtacttttt tggaaccggc aggtacgatt ttactctcgt
     4021 tgatttttcg tacgacgacc ggagcactaa ggtgcatttc gtgagcgacg gggacaagtt
     4081 cgtaaggttg aacggtttgt ggagcgccgg acagataatg gatttcggtg cggcatacct
     4141 cgtcgcgaaa ggctgtggcc ttgaggaacc ggccatagat atcgccggtt taagcctctc
     4201 cttcaacgac cgtttcaaag tcgaaaaaat ccacggaatg actctgataa gcgatttcta
     4261 caacagtagt ctcgagtcgt ggaggtcggc cgtcgattct ataaagaaaa tcgagtcgaa
     4321 aaggaagata gccgttgtgg gctcgatact tgaacagggt actcacagcg gcgatacaca
     4381 catggaactg ggaaagatac tcggagcgtt cgacgaagtc ctcttcttca acacggaccg
     4441 tgatatcgag atggcttgcg gaacggtgaa gccggttctc atcagcgatg atgtgagaga
     4501 gctggcttgg tggttgaagg agaacgcgaa gaaaggcgat ctgattttct tcaaggcctc
     4561 gcgcagcgtt tttctggaga gagtcttcgg aaagttcatg gagttgatta aagatggttg
     4621 atcaggttct ctcgttttta attccgctgg ttgcaacggc aatccttctg gaacccttca
     4681 agcggttcca gaggaaatac aagataggcc agtacatacg gcaggaggga ccggatctcc
     4741 acaattacaa aaccggtact ccgacggcct ccggcgtcat cttcataccg atctcgctga
     4801 tcgtcatgat ccttttttcc cggcaaccgg aaacgctcat cgtcgtgtcg gccggtatcc
     4861 tcttcggcgc agtcggtttg atcgacgata tttcgaagat agccaagaag aatgctgccg
     4921 gtttgagcgg caagagggag ttgctcctgc agttcgtatt tgcttctatc gtggtactta
     4981 tgattcagct tt
//
LOCUS       DAOOLY010000188         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20367_length_4976_cov_4.543385, whole genome shotgun sequence.
ACCESSION   DAOOLY010000188 DAOOLY010000000
VERSION     DAOOLY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20367_length_4976_cov_4.543385"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLD15_09075"
     CDS             <1..488
                     /locus_tag="PLD15_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489264.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPB64101.1"
                     /translation="GEISVAEEHLATSLMNRLMASTYSMIDTVPSHPRRKAVVLSSQN
                     EYHELGARILADLLELNGWNVSYLGANTPVSEVLKHLKKTIPFLLAISVTMSFNLDHT
                     KKLIQSVRFDSDFKGMKIMVGGLVINESEDLWKRLGADGTASSAAEAVEMAKLWWEER
                     E"
     gene            481..1416
                     /locus_tag="PLD15_09080"
     CDS             481..1416
                     /locus_tag="PLD15_09080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB64102.1"
                     /translation="MNDLREAILGSLDRFFNSLESFSGAILVLFDLEGRILDHSPSLS
                     ALIGGRKEFVGENFGSYIVDNAIKMMMKERTGLVRVGITDSRSNIHLVSGFVIKIDVS
                     RWLFFGEKIGLSAGDIMENMSKTTDELASLTRELAKKNKELQAANAEIERLMNCDPLT
                     GLSNRRDFMKKLDEEIESLCSTCKPLSIIMADIDDFKKVNDTRGHPEGDRVLQLFAEI
                     LNEIISPRRTTVRYGGEEFIVMLPETDGKEAAEIAEEVRINFERETEKQLGFKVTASF
                     GVATFRPEDTKASILKRVDDALYSAKSGGKNRVVR"
     gene            complement(1527..3140)
                     /locus_tag="PLD15_09085"
     CDS             complement(1527..3140)
                     /locus_tag="PLD15_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64103.1"
                     /translation="MFRKILVTVILLASVLVFSTQFKRSVEDKIVIEPGGNAVITRTE
                     YVPSSDLAKVYIQHSEALSDREVSDEVFNNFYDEISKGYFFLYSSVPGFVDRDIDISI
                     DKGGNYKSVITMKISGLITQSKEFPGSYEFSRKNFSEEKIMLKYFEDLIDGKAFETAF
                     LGSSKNSLVTTKTTTIVLPEESEIFELLSPHGEKPLKDWSLDLGGGTKFKASLDYDKN
                     TISLKEEITTGGGSPDNLMKPDNEEIMAKLRDYGAFTIVFKSPKTRALTEPKPYLFKE
                     DYSGSWSFYVSHTFSNEFSYQTLTVEPGLTVSLTFGASLLWEHEWKKISWWNYKYVLK
                     KFQTTVSFSPSLTPFIEVSSGAAITKNWTKNITTKTKWITFWVSCVPVALVMEVKFDA
                     NAEAGISGVIGFNASTTLSANTTLTVKYQNGWSKSVSYSANYSGLQFDANAKVNAWAK
                     GSLPLTLSAYVYYVAGPFVQFVPWIKGETNASAGSATQVGYKVTAGFDVNGGVHMAGW
                     LKDLCDGLPSVSYTFWNKSWTIANSTITF"
     gene            3579..4103
                     /locus_tag="PLD15_09090"
     CDS             3579..4103
                     /locus_tag="PLD15_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64104.1"
                     /translation="MPWIYQICRLFVEEGLLKLDMNGENVMALSPLQGLRFKERSGAF
                     RGEITQESGEIRLSLISDGGMKQFLPFIEPLSIDELSEYTGRYYCRDIDTGYDISVAE
                     RGLLLRNINPHHDGMNFEYRPAIKDIFYTFAPPYIACYFSVEFLRNHNNIIEAFVFRD
                     YDKDGREYLRFLKV"
     gene            4341..>4976
                     /locus_tag="PLD15_09095"
     CDS             4341..>4976
                     /locus_tag="PLD15_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB64105.1"
                     /translation="MKGFSELKFAGPKVISAVFLIMFLLMFLPFHVFESEVIQYTHKV
                     YSDIYQKAVDQFVTSISRGYFQWNAMFSAFISQDREFIDSMFQEILTDFPYAREIMTV
                     ERPSGMTTEKDYTVSMKNEELYAYVNIMDSLSQDIVDDVVVMVGIDKYRVLEGLSMVD
                     RVIFSDEGKKIFAYGLHASIRGYGLYNWLVFHAFISGLIGVLLMMEVLSVSF"
BASE COUNT         1313 a         1100 c         1272 g         1291 t
ORIGIN      
        1 cgggcgaaat atctgtcgca gaggagcatc ttgccacctc gctcatgaac aggctgatgg
       61 ccagcacata cagtatgata gataccgttc cctcccatcc aagaagaaag gcggtagttt
      121 tgagctcgca gaacgagtat catgagcttg gggctagaat tctcgccgat ctgctggagt
      181 tgaacggctg gaacgtcagc tatctgggcg cgaatactcc ggtttcggaa gttctgaagc
      241 atctaaagaa gactattccc tttttgcttg ccatttctgt cactatgtcg ttcaacctgg
      301 atcacacgaa gaagctgata caatcggtga ggttcgacag cgatttcaaa gggatgaaaa
      361 tcatggttgg cggactggtg ataaacgaat cggaagatct gtggaagaga ttgggggccg
      421 atggaacggc ttcctctgcg gcagaagccg ttgaaatggc gaaactctgg tgggaagagc
      481 gtgaatgatc tgagagaggc gatcctgggc agcctggaca gatttttcaa tagtctggaa
      541 agcttctcgg gagcgattct ggttctattc gacctggaag gtcgaatact ggatcacagt
      601 ccctcacttt ccgctttgat cggaggcagg aaggagttcg tcggagagaa cttcggcagt
      661 tatatcgtgg ataatgcgat caagatgatg atgaaggagc gtacgggtct cgtcagagta
      721 gggataaccg actcgaggtc caatatacat cttgtgagtg gcttcgtaat aaagatagat
      781 gtttctcgct ggttgttctt cggagagaag atagggctct ctgccggaga tatcatggag
      841 aacatgtcga agacaaccga cgagctggcc tctctcacga gggaactggc gaagaagaat
      901 aaggagctcc aggcggctaa cgctgaaatc gagagactca tgaactgcga ccctctcaca
      961 ggattgtcga acaggcgcga tttcatgaag aaactcgatg aggagatcga gagcctttgt
     1021 tctacatgca agcccctttc gataatcatg gccgatatag acgacttcaa gaaagtgaac
     1081 gacacgagag gtcaccccga aggtgacagg gtactgcagc tgttcgccga gattctaaac
     1141 gagataatct caccgaggag gaccacggta aggtacggcg gcgaagagtt catagtcatg
     1201 ttgcccgaaa cagatggaaa agaggccgcc gaaattgccg aagaagtcag gatcaatttt
     1261 gaaagagaga cggagaaaca gctcggcttc aaggtaacgg ccagcttcgg agttgccacc
     1321 ttcaggcctg aagatacgaa ggcaagcata ctgaagcgcg tggacgacgc cctttacagc
     1381 gcgaagtctg gcggaaaaaa cagagtagtg cgttaagaac aaatccaggt gccttcggca
     1441 gccagttttt gtttcgaaaa tgtaagttcc aaagagcggg atgcagtatc acaatccggc
     1501 atgacgggac agtacatccc ggcgctctag aaggtaatag tcgaattggc tatagtccag
     1561 ctcttattcc agaatgtgta gctaacgcta ggcaagccat cgcaaagatc tttaagccag
     1621 ccggccatgt gtactccgcc gtttacgtcg aagcccgccg tgactttgta gccgacctgc
     1681 gtggcgctcc cggcagaggc attcgtttct ccttttatcc atggcacgaa ctggacgaaa
     1741 ggtccggcga catagtagac atatgccgat agagtcaacg gtagcgaacc cttggcccag
     1801 gcgttgactt ttgcgtttgc gtcgaactgg aggcccgagt agttggccga gtaggaaacg
     1861 ctcttgctcc acccgttctg atacttgact gtgagcgttg tgtttgccga gagtgttgtc
     1921 gaggcgttga agcctattac accggaaatg ccggcctccg cgttcgcgtc gaattttact
     1981 tccataacga gcgccaccgg aacacaggat acccagaagg ttatccactt ggtcttggtt
     2041 gttatgttct ttgtccagtt ctttgtgatt gccgctcccg atgagacttc aatgaacggg
     2101 gtcaatgaag ggctgaagct cacagtcgtc tggaatttct tcaagacgta tttgtagttc
     2161 caccagctga ttttcttcca ttcatgctcc cacagaagcg acgcgccgaa tgtcaggctg
     2221 acggttaatc caggctcaac cgtcaatgtc tgatacgaga attcgttcga gaaagtatga
     2281 gaaacataga aactccacga accggagtaa tcttccttga agagatacgg tttcggttcg
     2341 gtcaatgcgc gggttttcgg actcttgaag acgatggtaa aggcgccata gtctctcagc
     2401 ttcgccatga tctcttcgtt gtccggtttc ataaggttgt ctggtgaacc gcctccggta
     2461 gttatttcct ccttgaggga gattgtgttc ttgtcgtagt caaggcttgc cttgaacttc
     2521 gtacctccgc ccagatctag cgaccagtct ttgagaggtt tctcgccgtg aggtgatagt
     2581 aattcgaaga tctccgattc ctctggaagt acaatcgtag ttgtctttgt agtaacgagc
     2641 gagttcttgg aggaacccag aaaggccgtc tcaaaagcct ttccgtctat caggtcttcg
     2701 aagtacttga gcatgatctt ctcttcggag aagttctttc tcgagaactc gtaacttccc
     2761 ggaaattcct tggattgtgt gataagcccg ctgattttca tcgtgataac gctcttgtaa
     2821 ttgccgccct tgtcgatcga gatgtctatg tctctgtcta cgaaaccggg cacagatgag
     2881 tagagaaaga agtagccctt tgagatttcg tcatagaagt tattgaagac ttcatcactc
     2941 acctcgcgat cggaaagagc ttcactgtgc tgaatgtaga ctttcgccag atcagaggaa
     3001 gggacatatt cagtacgggt aatgaccgcg ttaccgcctg gctcgatcac gattttgtcc
     3061 tctacggatc gcttgaactg tgtagaaaag accagtaccg acgctagaag aataaccgta
     3121 accagaatct ttctgaacat gcttgcaccc cctttcaagt catacgtctg agtatagcat
     3181 cgtcggtatg tgtgtagtct caagttatgc gacagtaatt tcattaattt gagacttatt
     3241 tttcaaactc acaggtttag gcaaagagct ctcgtggaaa atacagacag acaatgaact
     3301 gacgagggac ttgttcgatt ccccattctt gttgggctac gttcgccacc tttctcacgt
     3361 acaaggccgt ctggataggt aaagaagtac gtaagatagc caggtggtat ccatcctcta
     3421 aaaccttttc cgggtgtggt ttcaagagag agtatttccg acagagaggg aagacaacaa
     3481 caaactgacg aaaaatatag tatcgggtta ctgcaactac aactctctgc atccggagtc
     3541 tttcaaagat acgaccagcg aaccaggagg agtctgtaat gccctggatc taccagattt
     3601 gcaggctttt tgttgaagaa ggtttgctga aactggatat gaacggcgaa aatgtaatgg
     3661 cgctatcacc cttgcaggga ctgcgtttca aagaaagaag cggagctttc agaggcgaga
     3721 tcacacagga gtctggagaa atcagacttt ctttaatcag cgatggtggc atgaagcaat
     3781 ttctaccctt tatcgagccg ctatctatag acgaactctc ggagtacacg ggaagatatt
     3841 actgccggga tatagacacg ggctacgata tctctgtcgc tgaaaggggc ttgctcttgc
     3901 gcaacataaa cccgcaccac gatggtatga atttcgaata ccggccggcg ataaaggata
     3961 tcttctacac ttttgcgccg ccgtacatcg cctgctactt cagtgttgag ttcttgagaa
     4021 accataacaa tatcatcgaa gcttttgtct tcagagatta cgataaggac ggcagagagt
     4081 acctgagatt tttgaaagtc tagcactctg aaataactat atcctgcaaa aagtgaaaag
     4141 ccttttcatc gcttcgcgca gtaatttcga agcgatttcc tccggtgtta cgatactctt
     4201 tgcaacattt ttggagagag tctatcggaa atactggaat tgaatattcg tatgatatat
     4261 taattggaat gatactttag attaattcga aagactcaac cataaatgaa ctgggagtgt
     4321 ggaacatagg aggaattcca atgaaaggat tttcggaact gaagttcgcc ggtcccaaag
     4381 tgatttctgc cgtctttctt atcatgttct tgcttatgtt tttgcctttt catgttttcg
     4441 aatcggaagt cattcaatac acccacaaag tttactcgga tatctaccag aaagctgtcg
     4501 atcagtttgt tacgtcgata tccagaggtt atttccagtg gaatgccatg ttcagcgcat
     4561 tcatatcaca agaccgggag tttatagata gcatgttcca ggagatactg actgactttc
     4621 cttatgctag agagatcatg acagtcgaaa gaccttcggg aatgacgacg gaaaaggact
     4681 acacggtatc tatgaaaaac gaagagcttt atgcgtatgt aaatataatg gacagccttt
     4741 cacaggacat cgtagatgat gtggtcgtca tggtgggcat cgacaaatac agggttcttg
     4801 agggcctctc catggtcgat agagtcatct tttcagacga agggaaaaag atatttgcgt
     4861 acgggcttca tgccagcata aggggctacg ggctctacaa ctggctggta tttcacgcct
     4921 tcatctccgg attgataggc gttcttttga tgatggaagt tttgtccgtc tctttt
//
LOCUS       DAOOLY010000189         4949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20506_length_4949_cov_4.581324, whole genome shotgun sequence.
ACCESSION   DAOOLY010000189 DAOOLY010000000
VERSION     DAOOLY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20506_length_4949_cov_4.581324"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1259
                     /locus_tag="PLD15_09100"
     CDS             300..1259
                     /locus_tag="PLD15_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64106.1"
                     /translation="MEAIYASELVKKFGELRALDSLSIEIEKGDFFCLLGPNGAGKST
                     FIRVASGLMRPTSGLVRVAGMDVTTSLSKVRRKVSLVSEKVILYDNLSPVENLLFFAS
                     MSGKGRKAALRKILELLERVDMLDWKDKPVRVFSTGMRQRINFVRALINEPEIIFMDE
                     PTLGLDPHTTITIREMVGEVNGSGTTVILTTHLMREAEGMARHIGIMHRGKMLASGDL
                     ESLRKIIGGDLVRFKLSGESVGDLTDIEGYIGDRQLDGYRLLEVKPSTSIEKVVFSLS
                     SRGARLFDIEHVRPTLEEVFIELTGEEGCPNRPKNQRSTITNI"
     gene            1288..1500
                     /locus_tag="PLD15_09105"
     CDS             1288..1500
                     /locus_tag="PLD15_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HPB64107.1"
                     /translation="MTDIKRNDTEIIRVSKREFKGHEFLDLRIYYEGDDGQYKPTKKG
                     ITLNPKLVGELIEALNKEKDSEPERE"
     gene            complement(1668..2861)
                     /locus_tag="PLD15_09110"
     CDS             complement(1668..2861)
                     /locus_tag="PLD15_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB64108.1"
                     /translation="MIEKMERNVRVYNRFRFSQNMLIIGPVLVPYMIFKGLSYSEIML
                     LQSISAISVVLFEVPTGSLADRISRKFSLVISSLIMGVGLSMYIFMNGFAAFAIAEVL
                     FGLGLTFSSGADSAILYESLSRIDRKGDYSNIEAASMSYIFVGQAVGSIASGFLYSLS
                     PYIPFWISVAMLFLSGIFSLMFVEPPERMKSRQSYLVHIVRCFNTAFHKPRVRWAMYF
                     AALMGVMLRATFWLYEPYFKLINLDVAFYGVVFAVFNLVSAFSSRYMVKAVESVRPRK
                     VLIALGFMISGAYLLPLLFRGFFSIVLLLPGQLVRGMYTPVVRFYVNNQVEDSFRATM
                     LSIVSLSANLSFSIFSPLIGAGLDGIGTVPVYLTMGVTAAVVNLSLLQLRRVNKRKGA
                     VTGGA"
     gene            complement(2929..3525)
                     /locus_tag="PLD15_09115"
     CDS             complement(2929..3525)
                     /locus_tag="PLD15_09115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB64109.1"
                     /translation="MNFDEWKNRLEQLAKEGPFAIADMDIDKFAAINSEFGRDCGDRV
                     FEILDRTLKENLPDKLSYVRFGDEFFVYSDEYTLETLFMEMQDLKHTIANLSFECKGK
                     TVRFTVSGSVGEYPRNASTVEKLLNLLSEGMRKAKMGGRGQVIFAPLERESKMVLKSN
                     YYLKSQLDGLAKLTALLNRTESSLLREALDDLLRKYRL"
     gene            3659..4579
                     /locus_tag="PLD15_09120"
     CDS             3659..4579
                     /locus_tag="PLD15_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPB64110.1"
                     /translation="MIRLKFCAKILPIYFFCLAWTFATGFDIQGHRGCRGLMPENSLL
                     AFEHALKLGVDTIELDTVVTSDGVVVIYHDPFLNPERVRENGQFITERVLIKNLTLSR
                     LREYDIGSIAEPGKWPGQLQVDGQIVPTLEEVIQLVEVHNSLSEKKVRLNIEIKTSPL
                     EPELTVALHEHVEGVMDLVERYGMGELVIIQSFFWKVLKIVDEIDGNVLTAALISSTN
                     LDKTVWTDGLRLRQFGFDIGKMLASIGVDIVAPSYTIMADSWIQSAHKAGLKIIPWTV
                     NDTSEMKRLIELGVDGIITDYPDRLIQLLR"
     gene            complement(4672..>4949)
                     /locus_tag="PLD15_09125"
     CDS             complement(4672..>4949)
                     /locus_tag="PLD15_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HPB64111.1"
                     /translation="NSDMYPPHHVSGESPFVQTLLKCYELYTGNKGYCIAIGGGSYVH
                     RLKNGVGFGCTMPGTDNNMHGADEFAVIDELVTSAKIFTQAIIELCE"
BASE COUNT         1317 a         1197 c         1260 g         1175 t
ORIGIN      
        1 ggaagacagg atttgaacct gcgacctcct gtgcccaaga caggcgcgct tccatctgcg
       61 ctactccccg ttcacgaatc ctaatatatc agattttggc cctatcgtca atagtacagg
      121 attgtaatac tgctgcatat ttttacggga agcagcccgt aaaccatcac tcccaatagg
      181 catgtcaaag atctaggctg atatttagac gttaattgta taaagcggtt catggccatc
      241 gccgtgtgag ggggacgatc ggtgacgttg tagtagaata agtatcgagg tggtttattt
      301 tggaggcaat atacgccagc gaacttgtca agaaattcgg agagttacgg gccctggaca
      361 gtctttcgat agagatagaa aagggagact tcttctgcct tctcggcccc aacggcgcag
      421 gcaaatcgac tttcataaga gttgcatctg ggttgatgag accgacttcc ggtcttgtga
      481 gggtcgcagg catggatgtg accacatcgc tttccaaggt gcgccggaag gtttctctgg
      541 ttagcgagaa ggtaatactc tacgacaacc tctcgcccgt ggagaatctg ctcttcttcg
      601 cgtctatgag cgggaaaggg agaaaagcgg ctctgagaaa aattctcgag cttctggaga
      661 gggttgatat gctcgactgg aaggacaaac cggtgagagt cttctccacg gggatgcgtc
      721 agaggatcaa tttcgtgagg gcgctgataa acgagcctga gataattttc atggatgaac
      781 cgactctcgg cctcgacccc cacacgacga tcaccataag ggagatggtc ggagaagtca
      841 acggctcggg aacgaccgtt attttaacca cccatctcat gagagaggcc gaggggatgg
      901 caagacacat aggcataatg cacaggggaa agatgctcgc ctcgggtgat ctagaaagtc
      961 tgagaaagat tatcgggggc gatctagtgc gtttcaagct ctccggagag agtgtcggcg
     1021 atcttacgga tatcgaaggc tatataggcg acagacagct cgacgggtat cgcttgctgg
     1081 aggtaaaacc ttcgaccagt atagaaaagg tcgtcttttc tctgtctagc aggggtgcca
     1141 ggctgttcga tatcgagcat gtgaggccga cgctggagga ggtcttcatc gagctgaccg
     1201 gtgaggaagg gtgtccaaac agaccgaaga atcaacgatc cactataacg aatatttaga
     1261 ctaactccta gaaaggatgt gcgaaacatg accgatatca agcgaaacga caccgagata
     1321 ataagggttt ctaagagaga gttcaaaggc cacgaatttc tggatctgag gatctactac
     1381 gagggcgacg acggacagta caaacccacc aagaagggca taacgcttaa cccaaaactt
     1441 gtcggtgaac tcatagaggc cctgaacaaa gagaaggatt ccgaaccgga aagagagtga
     1501 aagaaccttc gaaccgaagg aggacttgaa tatacaatct tcgtaatccg tgccgaaaaa
     1561 tccgtgtcgc ctgtgggttg aaacctgctg gtgtaaaatc cggagtccgt tagtttaagt
     1621 ttcgcccgga ctccggattc cgttcaggtt cgccggaggc ttttccgtta cgcgcccccg
     1681 gtgacggctc cctttctttt atttaccctt ctcagctgta agagagaaag gttgacgacg
     1741 gccgccgtca cacccattgt cagatagaca ggaaccgttc cgatcccgtc tagaccggcg
     1801 cctatcaggg gtgagaagat cgagaacgaa aggttggcgc tcaaactcac tatcgagagc
     1861 atcgtggctc tgaaactgtc ttctacctgg ttgttcacgt agaacctcac caccggtgtg
     1921 tacatgcctc ttaccagctg tccaggcagg agaaggacta tggagaagaa accgcggaag
     1981 agcagaggta gaaggtatgc tccggagatc atgaatccca gcgctataag gaccttccgg
     2041 ggcctcacac tttccacggc tttgaccatg tacctcgaag agaaagcgct tacaagattg
     2101 aaaacggcga agacaacccc gtaaaaggct acgtcgagat ttatcagttt gaaatatggc
     2161 tcgtaaagcc agaaagtcgc cctgagcatc acacccatca aagctgcgaa gtacatcgcc
     2221 cagcgtactc ttggcttgtg aaaagccgtg ttgaaacacc tcacgatgtg aacaaggtag
     2281 gactgcctgg atttcattct ttccggcggc tcaacgaaca tgagtgaaaa gattcccgat
     2341 aagaatagca tcgccacaga gatccagaaa gggatataag gggagagaga atagagaaaa
     2401 ccgctcgcga tggagcccac ggcctggcct acgaatatat acgacattga tgcggcttct
     2461 atattgctgt aatcgccctt tctgtcgatt ctcgacaggc tctcgtaaag tatcgcagaa
     2521 tcggctcccg aactgaaagt aagtccgagg ccgaacagga cttcagcaat cgcgaaggct
     2581 gcaaaaccgt tcataaagat gtacatggat agaccaaccc ccattatcag ggaggagata
     2641 accagtgaaa attttctcga gatcctgtcg gcgagtgaac ctgtcggcac ttcgaagagc
     2701 acgacggaaa tagctgagat ggattgcaaa agcattatct ccgaataaga taaccctttg
     2761 aagatcatat acggcacgag taccggtccg attatcaaca tattctgtga aaaccgaaaa
     2821 cggttgtaaa cgcgaacgtt gcgttccatt ttctctatca tacgatcatc tcccgaataa
     2881 aaaaaggtgg ctcgccggcc acccgctatc gtttgtgggt aatactctct atagccggta
     2941 ttttctcaac agatcgtcga gcgcctccct caggagcgaa gactcggttc gattgagaag
     3001 tgctgtcagc ttcgccagtc cgtcgagctg ggatttaagg tagtagttcg acttgaggac
     3061 catcttcgac tccctttcga gcggggcgaa gataacttgc cctctccctc ccatcttggc
     3121 tttcctcatg ccctctgaca gaaggttgag aagcttctcg acggtcgatg cgttcctggg
     3181 atactctcca accgaaccgc tcacggtgaa cctgactgtc ttgcccttgc attcgaaaga
     3241 gaggttggcg atcgtgtgct tcagatcctg catctccatg aacagagttt ctaaagtgta
     3301 ttcatcgctg taaacaaaga actcgtctcc gaacctcacg tacgaaagtt tatcggggag
     3361 gttttccttc aaagtcctgt ctagtatctc gaagaccctg tcgccgcaat ccctgccgaa
     3421 ttcgctgtta atcgccgcaa atttgtcgat atccatgtcc gcgatcgcaa acggcccttc
     3481 cttggctaat tgctccagtc tgttcttcca ttcgtcgaaa ttcatattag tacctccata
     3541 tatatatagg gatatattac atgctatata ttactatatc ttataaacga cattaagttt
     3601 ctacgcaacg gccactacga ctgatagaat agttaggatg acacctaaac tcgtgggagt
     3661 gattcgattg aagttttgtg cgaagatact gccgatttac tttttttgtc tggcatggac
     3721 tttcgcgacc ggctttgata tccagggcca caggggctgc cgcggactga tgccggaaaa
     3781 ctctcttctt gcctttgagc atgccttaaa gctgggtgtt gacacgatag aactggacac
     3841 ggtcgtcacc tccgatggag tagtagtgat ctatcacgat ccctttctga atcccgaaag
     3901 ggtccgcgaa aacggacaat tcataactga aagggtgtta atcaagaacc tgacgctttc
     3961 tcggctcaga gagtatgata tcggcagcat agccgaaccc ggaaaatggc ccggtcagct
     4021 tcaggtagat ggtcagatcg tacccactct cgaagaagtc attcaactgg tcgaagtgca
     4081 caactcgctt tcggaaaaga aggtccgtct gaacattgag ataaagacct ccccgctgga
     4141 gccggagttg acggtcgctc tacacgagca tgttgagggc gtaatggacc ttgttgaacg
     4201 atacggaatg ggcgagctcg ttatcatcca gtctttcttc tggaaggtcc taaaaatagt
     4261 cgacgaaatc gacggaaatg tcctcaccgc ggcactgata tcctcgacca acctcgacaa
     4321 aacggtctgg accgacggat tgaggctgcg gcaattcggc ttcgacattg gtaaaatgct
     4381 cgcaagtatc ggggtggata tagtagcacc cagttacaca atcatggcag acagctggat
     4441 acaaagcgca cataaggctg gacttaaaat aatcccctgg acggtcaacg acacttccga
     4501 gatgaagaga ctgatagagc tgggagtcga tgggatcatc acagattatc cagacaggct
     4561 tatacagtta ctcaggtgag ttggatgtca gaataaagat aagaggcaag aaggtcaaga
     4621 tcttttgccc ttctcacctc tttcacaaaa atgaactttc ccgccttttc tttactcgca
     4681 gagctctatt atcgcctggg taaatatctt cgcactcgtg accagttcgt cgatcacagc
     4741 gaattcgtcg gctccgtgca tattgttgtc ggtcccgggc attgtgcatc cgaagcccac
     4801 accgttcttc agtcgatgaa cgtaagagcc gccgcctatg gcgatgcagt atcccttgtt
     4861 tccggtgtag agttcgtagc atttcaacag tgtctggacg aacggtgatt cgcccgatac
     4921 atgatgcggt ggatacatgt cgctgtttt
//
LOCUS       DAOOLY010000190         4945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20523_length_4945_cov_5.323517, whole genome shotgun sequence.
ACCESSION   DAOOLY010000190 DAOOLY010000000
VERSION     DAOOLY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20523_length_4945_cov_5.323517"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1167)
                     /locus_tag="PLD15_09130"
     CDS             complement(61..1167)
                     /locus_tag="PLD15_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB64112.1"
                     /translation="MEENIGGDTVDLSARLGKLSLSNPVMPASGPLTGDDKKMLSIAA
                     FGVGAMVTKTISTVAAKVPRPCIIATNNHVVNTELWSEFSYERWLEEFIPAFREKSDL
                     PLIVSLGYSPIDLEKLVPLFCKDADAFELSTHYVADDPSLMEELISAVKRNSDRPVFL
                     KLDPSVADPAAMVRAIEKAGGDGVVAINSLGPVYPYDAALKRSPMGSGDGFGWVSGPA
                     IKMLALSMVRRIREVTDLPVIGVGGIASAGDVVDFLSAGADAVQLLSGALIKGKDIYA
                     RIISSLPKALESRGFNSVVEAVSSVRQPAQSFEVRNPVIDHDRCTRCGLCVSVCPYFA
                     LSLDERVEIDGEACFGCGLCQSRCPVGAIGGVLI"
     gene            complement(1152..2450)
                     /locus_tag="PLD15_09135"
     CDS             complement(1152..2450)
                     /locus_tag="PLD15_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB64113.1"
                     /translation="MQLNNVSIYTNDGEFIERGYVTIEGDEIVAVRAGESRYEGENLD
                     FAGSLVMPSFVNAHTHIYSTLSRGMRLSPFNPGSFTELLEQLWWRLDRSLTLEELKIS
                     GYVAAIESLKSGVTTLFDHSASPNAISGSLETIAGSVNETGLRYCGAYEVSDRDGTKA
                     RDEGIKENMEFSRFNTPFRRGFFGLHASLTLSRETLSLVSKEISGSIPIHVHIAEGPE
                     DQERSLSLFDQRVLERFHRSGLMSPGSIYAHCIHTTPKERELIPGTGGSIALNVQSNT
                     NNGVGLPDWKGFLREGVETAIGNDGYGFNLCHDVRFALLTPHYLSRDPRVSGSPDLKD
                     TLFGANYRLATRTFGANLGTVREGSAADLVVIDYRSPTPVDAGNFLDHYFFGICDNIK
                     VTDAFVSGRHVLSGGRATLVDEEAIYSEARRLSANIWKRI"
     gene            complement(2437..3822)
                     /locus_tag="PLD15_09140"
     CDS             complement(2437..3822)
                     /locus_tag="PLD15_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HPB64114.1"
                     /translation="MIYDLALENGTVCNGEELFPANVYIRDEVIELITERKMKFQARQ
                     SLDCSGLFLLPGFIDPHVHFDLFLGKYTSRDDFDSGSALALSGGVTTVIDFLDPVRTF
                     EELKEAVQRRMRSSRVSRVDYAFHVTLAGNPEFTPQAIVEAAMNLGMPSIKVFTTYSG
                     SGRRTDDGTLLDLLALSRSSGLVVLAHAENDEIILSRERELRKDCDPTYRDLPHLRPA
                     IAELSEVAKLVLFSKYVDGQLYVVHVSSGDTVKALSLMNTEWKNNTVLETCPQYLMLN
                     DEALGGESGFLNTYCPPARSERERKLLVRGLLEGNIGSIGTDHCPFTTQDKRESFSSI
                     PFGTGSLGLSFSVLNTLFDGDLVKVASLLSINPARLLGLHPERGTLAPGRAANIAVVD
                     RERLYDYSRSPLTGSDHSLFEGISLRGEVVTTVMRGRPAFHENRVLLETGSGKFVERK
                     RVFWGDRSAAE"
     gene            complement(3819..4808)
                     /locus_tag="PLD15_09145"
     CDS             complement(3819..4808)
                     /locus_tag="PLD15_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPB64115.1"
                     /translation="MSSMLRGKDFITTQDFDRTEIDLMLDLSSDLKRRFAINDPTPLL
                     PYQTVFLMFFDQSTRTRNSMEAGITQLGGHAHYLDPSTMQTAHGEVPRDTAMILSRYG
                     HAIAVRHCKFKAGNQYLRTLARYSDVPILNLQDDIYHPMQVMADMMTIRERFGTNLNG
                     LKVAITWAYAESHLKPLSVPQSQILLFTRYGMDVRLAYPEGFDLMPDIVEQAKSNALG
                     SGAKFEILHDMDEAFKEADIVIPKSWGGFYVSENTDEIMTEVRKNRDWICTPERMKLA
                     DRRAVYMHALPADRGREVVDEVIDGPQSIVIDEAENRLHTAKAIMALTMGGRP"
BASE COUNT          985 a         1480 c         1372 g         1108 t
ORIGIN      
        1 actccacctc agccgggtcg tactcgcgcg agcaggttat gcacctgaga cggtacttgc
       61 tcaaatcagc acccccccga tcgcgccgac cgggcagcgc gattgacaga gcccgcaacc
      121 gaaacaggcc tctccatcga tctccaccct ctcatccagc gagagcgcga agtaagggca
      181 gaccgagaca cagaggccgc atctggtgca tctgtcgtga tcaatcaccg gattgcgcac
      241 ctcgaaggat tgggccggct gtcggacgct ggagacggcc tccacgacgc tgttgaagcc
      301 ccggctctcc agtgccttcg gaaggctgga aattattctt gcgtatatat cctttccctt
      361 tatcaacgct ccggaaagca gctgcacggc atcggctccg gcgctcaaga aatcgaccac
      421 atcccccgcg ctcgcgatac ctcccacgcc tataacgggg agatcggtga cctcgcgtat
      481 tctcctgacc atcgagagcg ccagcatctt gatcgccgga ccgctgaccc agccgaagcc
      541 gtcgccgctg cccatgggcg accgcttcaa tgcggcgtcg tagggataga ccgggccgag
      601 agagtttatg gccaccacgc catcgccccc ggccttctcg atcgcccgca ccatggcggc
      661 cggatcggcg acggaagggt cgagtttcaa aaaaaccggc cggtcgctgt ttctcttgac
      721 ggccgatatc agctcctcca tcagggaggg atcgtcggcc acgtaatggg tcgatagctc
      781 gaaggcgtcc gcgtctttgc agaagagagg gaccagtttt tcgagatcga tcggcgaata
      841 tcccagactg acgatcagag gaagatcgga cttctccctg aaggccggga tgaattcctc
      901 gagccatctt tcgtaagaaa actcgctcca aagctcggtg ttgacgacgt gattgttcgt
      961 ggcgattatg cacggccgcg gcactttcgc cgccacggtg gagatagttt tagtcaccat
     1021 cgccccgacg ccgaatgcgg ctatcgagag catcttttta tcgtcgcccg tgaggggccc
     1081 ggaagcgggc atcacgggat tggagagtga cagtttgccg agtcttgcgg acaggtccac
     1141 agtatcgccc cctatattct cttccatata ttggccgaga gtcttctggc ctccgagtag
     1201 atcgcttctt catcgaccag cgtcgccctg ccgccggaaa gtacgtgtct accggaaacg
     1261 aaggcgtccg taaccttaat gttatcacag atcccgaaga agtaatgatc gagaaagttc
     1321 ccggcgtcga ccggcgtcgg actccggtag tcgatcacga ccagatcggc cgcgcttccc
     1381 tcgcgaacgg ttccgaggtt ggcgccgaaa gtcctggtcg ccagacggta attggctccg
     1441 aaaagcgtat ccttcagatc cgggctaccc gacaccctcg ggtctctcga gaggtaatgg
     1501 ggcgtgagaa gggcgaacct cacatcgtgg cagaggttga aaccgtaacc gtcgttcccg
     1561 atcgccgttt cgacaccctc tcttaaaaag cccttccagt ctggcagacc gactccgttg
     1621 ttcgtgttcg attgtacgtt caatgctatg ctcccgcccg tcccgggaat caactccctc
     1681 tctttgggcg tggtgtggat gcagtgggcg tatatggagc ccgggctcat caggccgctc
     1741 ctgtgaaacc tctccagcac tctctggtcg aagagcgaaa gacttctctc ctggtcctcc
     1801 ggcccctcgg cgatatgcac gtggataggt atcgagccgg agatctcttt cgagaccagc
     1861 gacagagtct ccctcgacag tgtcagcgag gcgtgcagtc cgaagaagcc ccgccggaaa
     1921 ggcgtgttga agcgtgaaaa ctccatattc tccttaatgc cttcatcgcg tgccttcgtg
     1981 ccgtctctgt cggagacctc gtaggccccg cagtacctga ggccggtctc gttgacggac
     2041 ccggcgatcg tctccagaga gccgcttatc gcatttggcg aggcgctgtg atcgaaaagc
     2101 gtcgtgacgc cgctcttgag cgactctatc gcggcgacat agccgcttat cttcaactcc
     2161 tccagcgtga gcgaacggtc gagcctccac cagagctgct ccagaagttc ggtgaaagag
     2221 ccgggattga agggagagag cctcatcccc ctggagagag tcgagtatat gtgagtgtgg
     2281 gcgttcacga aggagggcat cacgagactg cccgcgaaat cgagattctc gccttcgtac
     2341 cggctctctc cggccctcac cgcgactatc tcgtcgcctt cgatcgtcac ataaccgcgc
     2401 tctatgaact caccgtcgtt ggtgtatatg gaaacgttat tcagctgcac ttcgatcccc
     2461 ccaaaaaact ctctttctct ctacgaactt tccgcttccg gtctccagca agaccctgtt
     2521 ttcgtggaag gccggccttc ccctcatcac ggtcgtcact acctctcccc tcagtgaaat
     2581 accctcgaag agcgagtgat cactccctgt cagaggcgac ctggaatagt cgtataacct
     2641 ttccctgtcc acgacggcta tgttggccgc ccgtcccggt gccaacgttc ccctttcggg
     2701 gtggagaccg agcagccggg ccgggttgat cgaaaggagc gaggccacct tcacgagatc
     2761 gccgtcgaag agagtgttca atacggaaaa ggagagtccc agcgatcccg tgccgaaagg
     2821 tatcgaggaa aagctctctc tcttgtcttg cgtcgtgaag ggacagtgat ctgtgcctat
     2881 cgacccgata ttcccctcga gaagcccgcg caccagaagc ttcctctccc tctcgctcct
     2941 tgccggggga cagtaagtgt tcaaaaagcc cgactcgccg ccgagggcct catcgttcaa
     3001 catcaggtat tgtgggcatg tctcaagcac agtattattt ttccattctg tattcataag
     3061 tgacaacgcc ttcaccgtat ctccgctgga gacgtgcacc acgtaaagct gtccatcgac
     3121 gtattttgag aacagtacca gtttcgccac ctccgagagc tcggctatcg ccggccgtag
     3181 gtgtggcaga tccctgtatg tcgggtcgca gtctttccgc agttctctct ctcgcgacag
     3241 tattatttcg tcgttttcgg cgtgggcgag gacgactagg ccgctagacc tcgaaagggc
     3301 gaggagatcg aggagtgttc cgtcgtcggt cctcctgccc gagccgctgt aggtggtgaa
     3361 gactttgatc gacggcatcc ccagattcat ggccgcctcg actatcgcct gaggcgtgaa
     3421 ctcggggttt ccggccaggg ttacgtgaaa ggcgtaatcg acccggctga ctctggaaga
     3481 tctcattcgc ctctgcacgg cctctttcaa ctcttcgaac gttctcaccg ggtcgagaaa
     3541 atcgatcacg gtcgttaccc cgccgctaag cgccagggcc gaccccgaat cgaagtcgtc
     3601 gcgggaggtg tatttcccca ggaagagatc gaagtgaacg tgggggtcta tgaagcctgg
     3661 gagcaaaaaa aggccagagc agtccagcga ctgcctggcc tggaatttca tctttcgctc
     3721 tgttataagt tcgatgacct cgtcccggat atacacgttc gcggggaata gctcctcccc
     3781 gttgcagacc gtcccattct ccagggcaag gtcgtagatc atggcctgcc gcccatcgtc
     3841 agggccatta tcgctttggc cgtgtggagc ctgttctcgg cctcgtctat cactatcgat
     3901 tgcggaccgt ctatcacttc gtccaccact tctctgcccc tgtcggccgg gagcgcatgc
     3961 atgtaaacgg ccctcctatc ggccagtttc atcctctcgg gtgtgcagat ccagtccctg
     4021 tttttcctga cttccgtcat gatctcgtcg gtgttttccg agacgtagaa gccgccccag
     4081 gacttcggaa tgactatgtc ggcctccttg aaggcctcgt ccatgtcgtg aaggatctcg
     4141 aatttcgcgc cgctaccgag ggcgttcgac ttcgcctgtt ccacgatgtc gggcatgaga
     4201 tcgaagcctt cggggtaggc gagcctgacg tccatcccgt atctggtgaa gagaagtatc
     4261 tgggactgcg gaacggaaag aggcttcaga tgactttcgg cgtaagccca ggttatcgcc
     4321 accttcaatc cgttgagatt ggttccgaag cgttcgcgaa tagtcatcat gtcggccatc
     4381 acctgcatcg ggtggtagat gtcgtcctga aggttaagta tcggaacgtc ggagtatctg
     4441 gccagggttc tcaaatactg gtttccggcc ttgaacttgc agtgcctcac ggctatcgcg
     4501 tggccgtacc tggaaagaat catggccgta tctctgggaa cctctccgtg tgccgtttgc
     4561 atcgtggaag gatcgaggta atgggcgtgg cctccgagct gcgttatgcc ggcctccatg
     4621 gagtttctgg tcctcgtcga ttgatcgaaa aacatcaaaa agacagtctg gtaaggcagt
     4681 aggggagtag ggtcgttgat cgcgaacctt ctcttcagat cggaggacag gtccagcatc
     4741 agatcgatct cggtcctgtc gaaatcctgc gtggttatga aatcttttcc tctaagcatg
     4801 ctgctcatat tttacctcca gctctattgt gaaagcctca agggcaatag tgcgtacatc
     4861 gccgccgccc tgaccaggtg atcgaccggg cattgatcgt cgggactgtg ggcgtagatc
     4921 tcgttggccg gcccgaagcc gatcg
//
LOCUS       DAOOLY010000191         4944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20535_length_4944_cov_5.168951, whole genome shotgun sequence.
ACCESSION   DAOOLY010000191 DAOOLY010000000
VERSION     DAOOLY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20535_length_4944_cov_5.168951"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLD15_09150"
     CDS             <1..265
                     /locus_tag="PLD15_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64116.1"
                     /translation="SIETIRQSLDLVKAFREHEVRITLYPPYIKREDFAELAELCRGA
                     SRIALQQYRRIEGNDQEPYPSGVLEEFSGVLSRISGAKIVLRE"
     gene            complement(323..1513)
                     /locus_tag="PLD15_09155"
     CDS             complement(323..1513)
                     /locus_tag="PLD15_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPB64117.1"
                     /translation="MKRVMVLAVFAVLVVSLFGVNRIVEITVEGNVKTLASTVRNLSG
                     VKIGDEFDIEKIYRAKRTLQDSGLFTDVYMQIKPVENDYRLVITVEETNTIYPYLGQY
                     LTIGVGERNLLGTGVNIYGGLRFLRFTDKKFLGFIPQPQLFWGGFTIGAGYAQALGTG
                     IDFITELTLFKEVPWQTDLLLTLNRFKLGASYPFGDYVAGVSYTFENATFDASPANDY
                     SISIISGALAFGDVENYRQERANLYGHTVLSYGFGKGYSYLQQTAELNFWYRIIGRIY
                     ILSTTRVGVTYLGTAPVTKQFYIGGSTDLKGWNAYSFNPGHFVFEAVEAGVPLTESFG
                     IAPSDELSAYIPKLYLMGVAGTDKTLGLSIGIGFEWRTPLGIAFEPQIFFGGDGFKFY
                     FELR"
     gene            complement(1522..2766)
                     /locus_tag="PLD15_09160"
     CDS             complement(1522..2766)
                     /locus_tag="PLD15_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64118.1"
                     /translation="MKKYIASVFLLLLVFTGIGKVTLTFWHSMSDYQKPIIDRLVNDF
                     NRSQNEITVKAVFQGSYDDTVTKLKAALLTGKGPDVSMINIEHLQIFTKDGSLLDLTD
                     LLANDPAISPEDFVDNFWTTIIRDGKPYALPFNISALMLFYNKDHFRAAGLDPDKPPR
                     TWEEMEEYARKLTIKKPSDSRPSQYGLLWGVDAMFYQFEPLVWQNGGEIFNEDMTECV
                     INSAEGIEALERWQGWFLEGLSPIDLTIDEGIQSFTMGKISMGPMTSGGIRYALENIP
                     WNLGVAPLPENKQRTTTLGGASLSIMSGLSDEKVKAAWTFLKWMVSEKNTLFWYEGTG
                     YMPVKKSAMESLEIQLIWQRFPQLKAPIESIEFARPRPVHTNIIEIRNILYNSIEQVR
                     KGVTEPEKAFNEAVEKVNGLLK"
     gene            complement(2787..3608)
                     /locus_tag="PLD15_09165"
     CDS             complement(2787..3608)
                     /locus_tag="PLD15_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64119.1"
                     /translation="MNRRRVKTLKYIILIAGALVMIFPFFWSLMTSFKDLREILQNPF
                     SLLPRQFTFKNYLSVFRKVPFARYLLNSAVVASVTTLLQLGTASLAAFAFARMRFKGR
                     DLLFYTFLATMMVPQQVVMIPQYLVVMSLNLENSYTGLIIPHAATAISIFFLRQFFLT
                     IPQDLEDAATIDGCGALRILTNVFLPLTRPAMATIAIFSFMWSWNNYFWPLLVISEPE
                     MRTVQLGLAMFKSEGGIQWGEFMAATVVATLPIMIAYFIAQKQFVKGITLTGLKG"
     gene            complement(3601..4536)
                     /locus_tag="PLD15_09170"
     CDS             complement(3601..4536)
                     /locus_tag="PLD15_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64120.1"
                     /translation="MRKRITPYLFILPAGAVIFFFLLYPLVYSFFISLHEWDLSPGMT
                     FVGLGNYAKILSSPEFWDSMLYTAYYMVGVLPFSLVLGFLFASLLNDATLKGIGFFRT
                     VYFLPVVTSPIAAGMGFSFLFSTELGYVNHFIRSVGGDYVNWLTNPEGIMQILFNWTG
                     IQLPKILQGPSVALFVIILMGIWQNVGYAMVVYLAGLQGIPETYYEAASLSGATRFQC
                     LRKITIPLLSPTTFFLLIMFSINSFQVFGPVMVMTPEGGPLNTTSVVVFRLYREAFAY
                     YRFGYAAAMAFVLFIFVISMTAFQVKTLEGRVHYE"
     gene            complement(4680..>4944)
                     /locus_tag="PLD15_09175"
     CDS             complement(4680..>4944)
                     /locus_tag="PLD15_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB64121.1"
                     /translation="ASYVLHGHKIDGRVRFYGETKRICKKELLLHEFFPNKNPLVSFV
                     EFLERSDYRNFVSTAYEELKRFYPVVYRVRLSATSGWYICRCK"
BASE COUNT         1267 a         1289 c         1207 g         1181 t
ORIGIN      
        1 gtccatcgag accataagac agagtctcga tctggtcaaa gccttcagag aacacgaagt
       61 acggatcacg ctctaccctc cctacataaa gcgggaagat ttcgcggaac tggccgaact
      121 gtgcagaggg gcctccagaa tcgccctcca gcagtatcgc agaatcgagg gaaacgatca
      181 agaaccatat ccatccggag ttctggaaga attttccggg gttctttcaa ggatatccgg
      241 agcgaagata gttctgagag aatgatgaat cacagtcata aagaaaccgg ggactaccgt
      301 ccccggtttt gtttgatttg gactaacgta gctcgaagta gaacttgaac ccgtcacctc
      361 cgaagaatat ctgaggctcg aaagcgatgc ccagaggtgt tctccactcg aaacctattc
      421 cgatcgacag tccgagggtt ttatccgtcc ccgccactcc cataagatag agcttgggaa
      481 tgtaggcgct cagttcatcg gagggggcta tgccgaaact ctccgttaga gggactccgg
      541 cttccacggc ttcgaagacg aagtggccgg gattgaacga ataagcgttc cagcccttga
      601 gatcggtgct gccaccgatg tagaactgct tcgttaccgg ggcagtcccc agataggtga
      661 ccccgaccct ggtagtgctg agtatgtata tccttccaat tatcctgtac cagaagttca
      721 gctctgccgt ctgctgcagg taactgtatc ccttgccgaa gccgtagctg aggaccgtgt
      781 ggccgtacag attggccctc tcctgccggt aattctcaac atcgccgaag gctaaggcac
      841 cgctgattat cgagatggag taatcgttcg ccggggaagc gtcgaaagta gcgttctcga
      901 aagtgtagga aactccggcc acataatcgc cgaaaggata actagcgccc agcttgaacc
      961 tgttcagggt cagtagaaga tcggtttgcc agggaacctc cttgaaaagc gtgagttcgg
     1021 ttatgaaatc gatccccgtt ccaagcgcct gcgcgtatcc agctccaata gtgaaacctc
     1081 cccagaaaag ctgcggttgc ggaataaaac ctagaaattt cttatcggtg aacctgagga
     1141 atcggagacc tccgtatatg ttcactcccg ttccgagcag atttctctct cccaccccga
     1201 tcgtcagata ttgaccgagg tacggataga tcgtatttgt ctcttcaacc gttatcacaa
     1261 gcctgtaatc gttttcaacg ggtttgatct gcatatagac atccgtgaag agaccgctat
     1321 cctgcaaagt ccgcttggcg cggtagatct tctctatgtc gaattcatcg ccgattttca
     1381 caccggagag attcctcacg gttgaggcca gcgtcttaac attaccctct accgttattt
     1441 ccactatcct gttcactcca aacagcgaaa cgactaacac ggcaaatact gctagtacca
     1501 tcactctctt catagtccac ctcacttcaa aaggccgttg actttctcaa cggcttcgtt
     1561 gaaagccttt tccggctcgg taactccctt tctgacctgt tcgatggagt tatagagtat
     1621 gttccttatc tctattatgt ttgtatgaac gggtctcggc ctggcaaact ctatagactc
     1681 gatgggagcc ttcaattgcg ggaaacgctg ccagattagc tggatctcca gagactccat
     1741 agcagacttc ttcaccggca tataccccgt cccttcgtac cagaagagag tgtttttctc
     1801 cgagaccatc catttgagga aggtccaggc cgctttcacc ttctcgtcgg aaagacccga
     1861 cattatcgac agcgaggccc ctccgagcgt tgttgtcctt tgcttgttct cggggagagg
     1921 ggccactccg aggttccagg ggatgttttc cagggcgtac ctgatccccc cgctggtcat
     1981 aggtcccatc gagatcttgc ccatggtgaa agactggatc ccttcgtcga tggtaagatc
     2041 gatcggtgaa agcccttcaa ggaaccagcc ctgccacctt tctagtgcct cgatcccttc
     2101 ggccgagttg atcacgcatt ctgtcatatc ttcgttgaaa atctcgccgc cgttctgcca
     2161 gaccagaggt tcgaactggt aaaacatggc gtccaccccc cacaggagcc cgtactggga
     2221 cggtctggaa tcggaaggtt tctttatcgt cagctttctt gcgtactctt ccatctcttc
     2281 ccacgttctg ggcggtttgt cgggatcgag gccggccgcc ctgaaatgat ccttgttgta
     2341 gaagagcatg agcgcgctta tgttgaaagg caaagcgtaa ggtttcccgt ccctgatgat
     2401 cgtcgtccag aagttgtcca cgaaatcctc cggggatatg gccggatcgt tcgccagaag
     2461 atcggttaaa tcgaggagcg aaccgtcttt ggtgaagatc tgtaaatgct cgatatttat
     2521 catggagaca tcgggtccct ttccggtcaa aagcgcggcc ttcaacttgg tgaccgtatc
     2581 gtcgtacgaa ccctgaaata cggccttcac cgtgatttcg ttctgagatc tgttgaaatc
     2641 gttcaccagc ctgtctatta tcggcttctg gtaatccgac atggaatgcc agaaggtgag
     2701 agtcacctta ccgataccgg tgaaaacgag caataagaga aaaactgaag cgatatactt
     2761 tttcatgcct gacctccttt tctctatcaa cctttaagac ctgttagcgt tatgccttta
     2821 acgaactgtt tttgagctat gaagtaagct atcattatag gtagagtcgc cacgactgtg
     2881 gcagccatga actcgcccca ctgaatgccc ccttcggatt tgaacatggc cagccccagc
     2941 tggacggtgc gcatctcagg ttcacttatc accagaagcg gccagaagta attattccac
     3001 gaccacatga aagagaagat ggcaatggtt gccatcgccg gccttgtcaa cggcaaaaag
     3061 acgttagtca gtatcctgag agccccgcaa ccgtcgatcg tggcggcatc ttcaaggtcc
     3121 tgcggtattg tgagaaagaa ctgcctgagg aagaatatgg agatcgccgt cgctgcgtgc
     3181 ggtattataa gacccgtgta ggaattttcg agattcaggc tcatgactac caggtactgc
     3241 gggatcatta ccacctgttg cggcaccatc atcgtcgcca ggaaggtgta gaagagcaag
     3301 tcccggcctt tgaacctcat tctggcaaag gcgaaagcgg ccagcgaggc cgtccccagc
     3361 tgaagaagcg tcgtgacgct ggccactacg gcactgttta ggagatacct ggcgaaaggc
     3421 acctttctga aaacgctcag atagttcttg aaagtgaact gccgcggtaa cagcgagaac
     3481 gggttttgaa gaatctccct caggtccttg aaggacgtca tcaaagacca gaagaaagga
     3541 aagatcatca ccaaagcgcc ggcgataaga attatatact taagcgtctt gactctcctt
     3601 ctattcatag tgaacccttc cttcgagagt tttcacctgg aaggccgtca tcgagatcac
     3661 gaagatgaag agtacgaagg ccatcgcggc cgcgtaaccg aagcggtaat aggcaaaggc
     3721 ctctctataa agtctgaata ccactaccga agtcgtattc agcggaccgc cctcgggcgt
     3781 catcaccatc accggaccga agacctgaaa ggagtttata ctgaacataa tcaacagaaa
     3841 aaaggttgtg ggcgagagga gaggtatggt tatctttctg agacactgga atctagttgc
     3901 tccgctcaga gaggcggctt cgtaatacgt ttctggaatt ccctgcaaac cagccagata
     3961 aaccaccatg gcgtaaccga cgttctgcca gattcccatg agtattatca caaaaagcgc
     4021 cacactggga ccctggagta ttttcggaag ctgtatgcca gtccaattga agaggatttg
     4081 cattattcct tcgggattgg tcagccagtt cacgtaatcc cctcccacgg acctgatgaa
     4141 atgattgaca taacccagct cggtgctgaa gagaaagctg aaccccatcc cggcggctat
     4201 tggcgaggtg actaccggga ggaaataaac agtcctgaaa aaccctatac ccttcaacgt
     4261 cgcatcgttg agcagtgagg cgaagagaaa acccagaacg agagaaaaag gcagaacacc
     4321 gaccatgtag taggctgtgt acagcattga atcccagaat tctggggaac taagtatctt
     4381 cgcgtaattt cccaggccca cgaaagtcat accgggcgag aggtcccatt cgtgaagact
     4441 tataaagaag gagtaaacga gtgggtagag aaggaaaaag aagataaccg cccctgccgg
     4501 caaaatgaag agatatggag ttatcctctt tctcatggtt catcccccag atcgtaccga
     4561 tagaagatat gaaacacctg cccttccatt tctttcgtcc tagataaata ctaacatctc
     4621 ggctccgcaa atccaccacc gataaccgga ttcctacgaa taaaacgtcg aaggtttctc
     4681 tatttgcaac gacatatgta ccagccgcta gtcgcggaaa gcctgactct ataaacaacg
     4741 ggatagaatc tctttagctc ttcgtacgca gtagatacga aattcctgta atcgctcctt
     4801 tcgagaaatt caacgaacga gaccagaggg tttttgttgg ggaagaattc gtgaaggagc
     4861 aactccttct tgcagattct tttcgtttcc ccgtagaacc tgacccttcc atcgattttg
     4921 tgtccgtgta acacataaga ggct
//
LOCUS       DAOOLY010000192         4918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20658_length_4918_cov_6.278635, whole genome shotgun sequence.
ACCESSION   DAOOLY010000192 DAOOLY010000000
VERSION     DAOOLY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20658_length_4918_cov_6.278635"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..786)
                     /locus_tag="PLD15_09180"
     CDS             complement(64..786)
                     /locus_tag="PLD15_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPB64122.1"
                     /translation="MNLKPSRRAFFIRYIIFPYYFVLGIIFYLNFDLSGIDGNNRLFL
                     IFGWIALTVVPGILLAFSRRRFGWFFWPSLINAAGWLVRFIFPENIYMDFKPVFDNGP
                     VLLFMIVSLIAIIYLELYRNTFKYDLSEKGVEISFGFFGANTHMIVSKHITNVMLKRN
                     FLELILGVGHVIPVTSSGIGTGDKGVVGGFTGDTGTKNVRGGAFIGSTASEKEFVANP
                     KNCIYGVSKPRKVMDSLKEMVL"
     gene            complement(776..3694)
                     /locus_tag="PLD15_09185"
     CDS             complement(776..3694)
                     /locus_tag="PLD15_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB64123.1"
                     /translation="MRSDIFISASAGTGKTYNLVKNYIEVFEEAFRHGEALDVHNVVA
                     ITFTNKAAKEMKDRVISRIDERIALGHPGKWKTLRNRLTYAWISTIHSFCERLLRESA
                     LFLGIDPGFQILSGMKRVMLEQQTVRGFFEENLERLEPLIELTGLDEAFKIFRDSLSK
                     LRHELAVHPSPAGYSSLELGEHAEKVMAGSECFCVNFRELLDRYEARSYRMGLVDFDQ
                     LLTKTRDLLVEMPQVREKYRERFKYILVDEFQDTDELQSEIVSLLRKGGMNYCLFVGD
                     AKQSIYRFRGADVTVFNRTMDEFKSGGLEPLHLAVNRRSHPTLVEFQNHIFQKIMPPD
                     YSGDYFRSVYEKEVEALPYESDEPLERIRLIRSETSDDSRTVAAAIRALLEEEIHFRR
                     KDGSCLKRKIKPGDIAILLRTFSRISNYERALEELNIPYYTIGSKNFYDRPEVAGPLA
                     WLDLLVDPLDDWNFVTFLLSPSFGCSLDDVLSLRSLPGSKGRPLYYALQESKDRKFLH
                     LKELFDKFAQLKHVLSPSEILERFVSEIDYLARLATLNGAERMIANVRKMLELARELD
                     RLGSSLRELSSNLKAFVDSSEESEASLETEESDSVKIMTVHKSKGLEFPVVVLADLFW
                     KEKGSLRRILFDDKGFIIVKEGSGRRSDEETVPGRLTHREEQKSFEEEKRTLYVALSR
                     ARDMLLMSANGRGNATRPWSRMLGGTLLDIESGELLPEMEDIVSHFEPVRLPPLPQKE
                     VEEGYGLPDDRYVLPVEDRSYVKYLSPTAITADIDDEFELSDSDGDAGLGRKSMDLGT
                     LAHSLLEPLGIRGMHGTSPSLGGILEGGMPATVDRIRFTREDLDGVKRVLDRLRGHRI
                     VKEIEESRRAMSEVHFQHGFRKYVLMGIVDKLYLKDGEWKIVDFKFARKNPSNIDKYR
                     FQMEFYLYMLRELLSPVEATLFFLKDGETETVRPARPGVFEKKLLEKIIGSGGDRGES
                     "
     gene            complement(3684..>4918)
                     /locus_tag="PLD15_09190"
     CDS             complement(3684..>4918)
                     /locus_tag="PLD15_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPB64124.1"
                     /translation="REKQQRLNLYLSITTAGEAIHFTLPESTIEGEPILPSPYLKDIL
                     DSSGATVQRRGLIAGKRQGLIPELKDAMSRLELDVAASKYFKGPFWPGLRERFGLQRL
                     EASLIDFNREFRWEVKDLEKLRKHLGQTFSFSRLKSYDDCPFLFFLSYVAGLDQKTDH
                     IFELTPLDEGNVYHAVLRDYFSGKGEEWEGSLAENLTKHLLHDSSVVFKFEFARLREI
                     LLDYITIRESKRPRLMSGEYYPFDFEKAFGLSGTARVEVIPGVYLRGKIDRVDLDETS
                     DSIYIIDYKRGNSGDKEQLILYSLAADTLFADSGYTVAGGVFKTLTGTTVNRAAFKVE
                     TVNGERVWRFVGGRGEGGDWSESDALAWVRGITEGLYAGKFAPVCLSQSGKCFNCRFK
                     KMKRVATWRDGKEFADEE"
BASE COUNT          989 a         1440 c         1281 g         1208 t
ORIGIN      
        1 agactgcgga tctggaggcc gggcgttcaa atcgccccgg gcacgccata tccctttctc
       61 acttcaaagc accatctcct tgagcgaatc cataactttt ctaggctttg aaacaccgta
      121 gatgcagttc ttgggattgg ccacgaattc cttttcggag gcagtgcttc ctatgaaagc
      181 tccgcccctg acgtttttcg ttccggtgtc gcccgtgaaa ccgccgacga ctcccttatc
      241 gcccgttcca attcccgagg aggtgaccgg tatgacgtgg cccaccccca gaatcagctc
      301 caggaagttt cgcttgagca ttacgttggt tatgtgcttg gacactatca tgtgagtgtt
      361 ggcgccgaag aagccaaagc ttatctcgac gcctttttcg ctgagatcgt acttgaaggt
      421 gtttctgtac agttcgagat aaattatcgc tatcaggctc acgatcatga agagcaggac
      481 cggcccgtta tcgaacacgg gtttgaagtc catgtatata ttctcgggaa agataaacct
      541 taccagccag cctgccgcgt tgatcaggga gggccagaag aaccagccga atctcctgcg
      601 cgagaaggcg agcagtatcc ccggtacaac cgtgagggct atccagccga agataaggaa
      661 gagcctgttg ttgccgtcga tccccgaaag gtcgaaattc aaatagaaga tgatgcccag
      721 aacgaagtag tacggaaaaa tgatatacct gataaaaaac gcccgtctcg atggcttaag
      781 attcacctct atcacccccg cttccgatta ttttctcgag cagtttcttt tcgaaaactc
      841 ccggcctcgc cggtctcacc gtttcggtct caccgtcctt gaggaagaaa agcgtcgcct
      901 cgaccggtga gagaagttcc ctcagcatat atagatagaa ctccatctgg aatctgtatt
      961 tgtctatgtt cgacggattc tttcgcgcga atttgaaatc gacgatcttc cattctccat
     1021 ccttcaagta tagtttatca acgattccca tcaatacgta tttccggaaa ccgtgttgaa
     1081 agtggacctc gctcatggcc cttcgactct cttctatctc tttgacgatc ctatggcccc
     1141 tcagcctgtc cagaacgcgc ttcaccccgt ccaggtcctc ccgggtgaat cttatcctgt
     1201 cgaccgtggc cggcatcccg ccctccagta tccctccgag agagggagag gttccgtgca
     1261 tgcctctaat ccccagaggt tccagcaggc tgtgggccag cgttcccagg tccatcgact
     1321 ttctgcccag tcccgcgtct ccatcgctgt cggagagctc gaactcgtcg tcgatatcgg
     1381 ccgtgatagc cgtcggggag aggtacttga cgtaagagcg gtcctcgacg ggaagcacat
     1441 acctatcatc cggcagcccg tagccttcct ccacctcttt ctgcggaaga ggcggaagcc
     1501 tgaccggttc gaagtgagag actatgtcct ccatctctgg cagcaactcg cccgattcga
     1561 tatccaggag cgttccgccc agcatcctcg accagggcct ggtggcgtta ccccttccgt
     1621 tggcgctcat gagtagcatg tctctggccc tcgacagggc gacgtacagc gttctcttct
     1681 cttcctcgaa gctcttctgc tcctccctgt gggtgagcct tccaggcacg gtctcttcgt
     1741 cgctcctcct gccggagcct tccttgacta ttatgaagcc cttgtcgtcg aagagtatcc
     1801 tcctgagcga acccttctct ttccagaaga gatcggccag cacgacgacc gggaactcca
     1861 gccccttgga tttgtggacg gtcatgattt tgacgctgtc ggactcctcc gtctcgagag
     1921 aggcctccga ttcctcgctc gaatcgacga aggccttgag gttggaggag agctcgcgca
     1981 gagagcttcc cagtctgtcc agttccctgg ccagttcaag catctttcta acgttggcga
     2041 tcattctctc ggcaccgttc agcgtcgcga gtctggccag atagtcgatt tccgagacga
     2101 acctttccag tatctcgctg ggagacagta cgtgcttcag ttgagcgaat ttgtcgaaga
     2161 gttctttgag gtgcagaaac tttctatcct tgctctcctg aagcgcgtag tagagaggtc
     2221 ttcccttcga tcccggaagg ctcctgagcg agaggacatc gtccagcgag cagccgaacg
     2281 agggcgagag aaggaaggtc acgaagttcc agtcgtccag cggatccacc agcagatcga
     2341 gccaggccag cggtccggcc acttccggtc tgtcgtagaa gttcttgctt ccgatcgtgt
     2401 aatagggaat gttaagctcc tccagcgccc gttcgtagtt cgaaatcctc gagaaggtcc
     2461 tgagcagtat cgcgatatcg cccggcttta tcttacgctt caggcagctt ccatcctttc
     2521 gcctgaagtg gatctcctcc tccagcagcg ccctgatggc ggccgccacg gttctcgagt
     2581 cgtcggacgt ttcgctccgg atcagcctta tcctctcgag cggctcgtcg ctttcgtaag
     2641 gtagggcctc tacctccttt tcatagacgg agcggaagta atcgcccgag taatcggggg
     2701 gcatgatctt ctggaaaatg tggttctgaa attcgaccag cgtcgggtgc gaacgcctgt
     2761 tgacggccag gtggagcggt tctagcccgc ccgatttgaa ttcgtccata gttctgttga
     2821 agaccgtcac atcggctccc ctgaacctgt agatggactg tttcgcgtcg cccacgaaga
     2881 ggcagtaatt catgcccccc ttcctgagga gcgatacgat ctcgctctgc agctcgtcgg
     2941 tatcctgaaa ttcatcgacc agtatgtatt tgaaacgttc cctgtacttc tccctgacct
     3001 gcggcatctc cacaagcaga tcgcgggttt tggtcagcaa ttggtcgaaa tccaccaggc
     3061 ccattctgta actgcgggcc tcgtatctgt cgagcagttc gcggaaattc acgcagaagc
     3121 actccgaacc ggccatgact ttttcggcgt gctcccccag ctccagactg gaatagccgg
     3181 cgggggaggg atgaacggcc agttcgtgtc ttagctttga aagcgagtct ctgaagattt
     3241 tgaaggcctc gtcgagtccg gtgagttcga tcaggggctc cagtctttca aggttctctt
     3301 cgaaaaagcc gcgaacggtc tgctgttcga gcatcaccct cttcatgccg ctcagtatct
     3361 gaaagcccgg atcgatcccc agaaagaggg cgctctcgcg gagaagtctc tcgcagaagg
     3421 agtgtatcgt ggagatccag gcgtaggtga gcctgtttct gagagtcttc catttgccgg
     3481 gatgtccaag ggcgattcgc tcgtcgatcc ggcttattac acggtctttc atctccttgg
     3541 ccgctttgtt ggtgaaggtt atggccacaa cgttgtggac atccagggcc tcgccgtgcc
     3601 tgaaggcttc ttcgaagacc tctatatagt tcttgacaag gttgtaggtc tttccggtac
     3661 ccgccgaggc ggatatgaag atgtcactcc tcatcggcaa actcctttcc atctctccat
     3721 gtggccacac gtttcatctt cttgaaacgg cagttgaaac acttgccgct ctgggaaagg
     3781 cagaccgggg cgaactttcc cgcgtacagt ccctccgtta tcccccttac ccaggccagc
     3841 gcatcactct cgctccagtc gccgccctct ccgcgacctc cgacgaagcg ccagaccctt
     3901 tcgccgttga ccgtctcgac tttgaaggcc gccctgttga cggtggttcc ggtcagcgtt
     3961 ttgaagacac cgccggcaac ggtatagccg ctgtcggcga agagcgtatc cgcggccagc
     4021 gagtagagaa tcagctgctc tttatctccg ctgttgcccc tcttgtaatc tatgatgtat
     4081 atagaatcgc tcgtctcgtc cagatcaacc ctgtcgatct ttcctctgag atagacgccg
     4141 gggatcactt ccacccttgc cgtgccgctg agtccgaaag ccttctcgaa atcgaacggg
     4201 tagtactctc cgctcatcag ccgtggcctc ttagattcgc ggatcgttat atagtccaga
     4261 aggatctctc tcaatctggc gaactcgaat ttgaagacga cactgctgtc gtggaggagg
     4321 tgcttggtga ggttctcggc cagcgagccc tcccactctt cgcccttacc cgagaagtaa
     4381 tctctcaaca cggcgtgata gacgtttccc tcgtccaggg gggtgagctc gaagatgtgg
     4441 tcggttttct gatcgagtcc ggctacgtac gagaggaaga agagaaaggg acagtcgtcg
     4501 taagatttga gcctcgaaaa agagaaggtc tgccccagat gcttgcggag cttttctaga
     4561 tccttcactt cccacctgaa ctccctgttg aaatctataa gcgaggcctc cagtctttgc
     4621 aggccgaatc tttccctcaa tccgggccag aagggacctt tgaagtattt cgaggcggcc
     4681 acgtccagtt ccagacggct catcgcgtct ttcagctcgg gaatcagccc ctgcctcttt
     4741 ccggctatca ggccgcgcct ttgaacggtc gcacccgagc tatccagtat gtctttgaga
     4801 tatggagagg gcaggatcgg ctcgccctct atcgtcgatt cggggagcgt gaagtggatc
     4861 gcctcgcccg ccgtcgttat ggagagatag aggttcaacc tctgttgctt ctcgcgcg
//
LOCUS       DAOOLY010000193         4898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20772_length_4898_cov_4.573198, whole genome shotgun sequence.
ACCESSION   DAOOLY010000193 DAOOLY010000000
VERSION     DAOOLY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20772_length_4898_cov_4.573198"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLD15_09195"
     CDS             <1..880
                     /locus_tag="PLD15_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPB64125.1"
                     /translation="GRVKALINENGKRIKEAHPSDPLQIIGFDEVPDVHSIVYVVDDL
                     DTSRGLSQYSKGQLQKEKSVTNKRHVRLEEFLSMTGESNEKKDLKLILKADSFGTVEA
                     LKQAMLKLENTDVRIEVVHSGIGSVNSSDVMLASASDAVIMGFKVKPDAQARRQAEVE
                     GVQIKVYEIIFNLIDDLKKALEGLLEPEEVEEVTGRGHVKKIFKISKVGTIAGVQLIE
                     GHVLKKGKMRVYRNNEEIADTDIEFLKHYKDDASRVDAPKECGIKAVGFDEFEDGDQL
                     EFHTKKSVRRTIDFNE"
     gene            complement(900..1508)
                     /locus_tag="PLD15_09200"
     CDS             complement(900..1508)
                     /locus_tag="PLD15_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPB64126.1"
                     /translation="MPDFKTHVTWGLFSYPVYMLVAMLIIEIAKLPIIVDSRIIGTGY
                     LLYILGSDLPDIDSKQALIKRTLEVMIAGVVSSIIYSSLISPKIQPILLSWIYSLPVA
                     VTISFSMAIICGIVTSKILDLLSHRGFFHTIWAGLLYGAIVLALLLPRSGASAGNFSY
                     TEIGFLSLAGTTGYFLHLLLDRVETSRKKRKRALSVQEKGPQ"
     gene            complement(1501..2016)
                     /locus_tag="PLD15_09205"
     CDS             complement(1501..2016)
                     /locus_tag="PLD15_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64127.1"
                     /translation="MSETARQTLKMFYPISIKNIRQLKAGDLIQYSGRVLLANENTID
                     KYRMYEKLEGTPLLNLSREILFFCASVETGLSQEPADIDPDDLEYLFMSGVTATAGLD
                     ISDKEMFDIYRRFSRVNFSLCNRKVLSPVDTDREVPEEIECGLSVSLKDVLFFVDVSS
                     SGEKFGGICDA"
     gene            complement(2009..2884)
                     /locus_tag="PLD15_09210"
     CDS             complement(2009..2884)
                     /locus_tag="PLD15_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4895 domain-containing protein"
                     /protein_id="HPB64128.1"
                     /translation="MIYYAGPESYRNRLERVYNSISGRFMEIGSLVNERSDKLDNAFG
                     HFMTLVVQTMRRDFPVLSIFVDSEGRGFCGLSNSSPFETASALYRFGKEEAEEMREAF
                     SDFFGDAELTFQRAIFQTPLKLYFLSYYGNERLLKREILKDSLRGKEYFRLPELIDGE
                     LIDICIKNYRRWLEFPSGEVFIFPVQNVLKIAFGLPKSERNIDKSVVMELSRLFRSEV
                     TRKYGTLRIASVTPDMRIDRPVATVFEIDLVRAREILPALPHLYEFYCGCVERTLASM
                     VDQIENYFPLKKRGE"
     gene            complement(2881..4056)
                     /locus_tag="PLD15_09215"
     CDS             complement(2881..4056)
                     /locus_tag="PLD15_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HPB64129.1"
                     /translation="MERAVFELGKESPFYNFLLLFVDRIPSSATRTVRLRVTSRGRFQ
                     LLYNPDVLGNKPLAFTKALLKHECLHIVNGHILIPVNRSREKVVWDLSMDAAVNQYIP
                     ELDAFSLPLDSLLSEGCGTDNERFFAGPPMAHPGETAEFYHSWAMDFMRKNRSIDLEL
                     LEGNLSRVDSHEDFGSFELPKEFIEDLLKQVVSETLEKARAGMPEGLEEVMKLVLDNP
                     VLDWRNVIRRFFGSSTYVGRYRTPMRPNRRYDDQPGWKSDYAAKLVVVVDTSGSVIED
                     EFNAFFGEIDAVTRITDSRIWLVQVDETVQSVMKYGKGMWRDLKLVGRGETDLQPAVD
                     YSQENLRPEGLIIFTDGYTDLPSVARRVLFVLPKSHNSDFASEARSIYGKSSVVFLS"
     gene            complement(4064..>4898)
                     /locus_tag="PLD15_09220"
     CDS             complement(4064..>4898)
                     /locus_tag="PLD15_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB64130.1"
                     /translation="EVNRAHRSIRNAIMQLLIDKRIHNHFLPEDSWIIAAANPPDEEY
                     DQVELITDPAFMSRFFHIELTPDPEEWLRWSKTQNVPDCVTRFIGEYPEFLSRDTIVS
                     MRLELRPSPRSWYKLGRVFGILESDEIEKYGYVLAAGIVGPEAARTFMSKIQGNQLLP
                     SPRTLLMELDDSILKRISQSDISGISASVMRITRHLSELDEMQVQEYFQMVPRIAGNL
                     LEIGRVIPRDSFFSIIRHIVHQMEGEKGLKRALFENLLEELAMDHDVMKFLQGSHESA
                     K"
BASE COUNT         1225 a         1246 c         1220 g         1207 t
ORIGIN      
        1 cggaagggtc aaggcgctga taaacgagaa cggcaagcgg attaaagagg cacatccctc
       61 tgatccctta cagatcatcg gtttcgatga agtaccggac gtccacagta tcgtctacgt
      121 tgtggacgat ctcgatactt ccagaggcct ttcccagtac agcaaaggcc agctacagaa
      181 agagaagtcg gtaaccaaca agcgccacgt tcgactggag gagttcctct cgatgaccgg
      241 agaatcgaac gagaagaaag atctcaagct catactcaaa gccgattctt ttggaaccgt
      301 cgaagctctg aagcaggcca tgttgaaact ggaaaataca gatgtacgaa tagaagttgt
      361 gcactcgggg ataggctcgg ttaattccag cgacgttatg ctcgcgtcgg cctccgatgc
      421 cgtaataatg ggtttcaagg tcaaacccga cgctcaagcc cgtcgccagg ccgaagtgga
      481 gggtgtacag ataaaggttt acgagatcat attcaatctt atcgacgatc tgaagaaagc
      541 gctagaaggc ttgctggaac ctgaagaggt tgaagaagtg accggccggg gacatgtaaa
      601 gaagatcttc aagatcagca aggtcggtac gattgccggt gttcaattga tcgaaggaca
      661 cgtcctcaaa aaggggaaga tgcgcgtcta tagaaacaac gaggagatcg ccgatacaga
      721 tatagagttt ctcaagcact acaaagacga cgcgagtagg gtcgatgcgc ccaaggagtg
      781 tggaataaag gccgtaggat tcgacgaatt cgaagatggc gatcagctgg agtttcacac
      841 caaaaagagc gtcaggagaa ccatagattt caacgaatag tggatcttga attttgaatt
      901 tattgagggc ccttttcctg tacggagaga gccctttttc ttttcttcct tgaagtctcg
      961 actctgtcca gtagaaggtg caggaagtac ccggtagtcc ccgcaagcga aaggaaacct
     1021 atctccgtat aagagaagtt cccggcagaa gcccccgatc tggggagcag aagagcaaga
     1081 acgatagcgc cgtagagcag cccggcccat attgtgtgaa agaaacccct gtggctcagt
     1141 aaatctagaa tctttgaggt cacaattccg caaattatcg ccattgaaaa ggaaatggtc
     1201 accgcgaccg gaagagagta gatccatgaa aggaggattg gttgaatctt tggtgatata
     1261 agcgaagaat atataatgga tgagactact ccggctatca tcacctcaag tgtacgtttt
     1321 ataagtgcct gtttcgaatc gatgtccggc agatcgctcc ccagaatgta gaggaggtaa
     1381 cctgtcccga taatccgact atcgacgata attggaagtt tggcgatctc tatgatcaac
     1441 atagcaacga gcatatacac cggataggag aagagccccc acgtcacgtg cgttttgaag
     1501 tcaggcatcg cagattcccc cgaacttctc gccgctgctg gaaacatcga cgaagaagag
     1561 aacatccttg agagatacac tgaggccaca ttcgatttct tccgggacct cccggtcggt
     1621 atcgacaggc gagagaacct tcctgttaca caacgaaaag tttaccctgg aaaaacgcct
     1681 gtagatatcg aacatctcct tatcgcttat atccagaccc gcggtcgccg taacgccgga
     1741 catgaaaagg tattctagat cgtcgggatc gatatcggcc ggttcctgac tcaaaccggt
     1801 ttcaacggac gcacagaaga agagtatctc tctggaaagg ttcagcaggg gcgttccctc
     1861 gagtttctcg tacatccggt acttatctat cgtattttcg ttggccagaa gcacacggcc
     1921 gctgtactgg ataagatcgc cagccttgag ctgccttata tttttgatgg atatcgggta
     1981 gaacatcttc agggtctgtc tggccgtctc actcaccccg cttcttcagg ggaaaatagt
     2041 tttcgatctg atctaccata cttgcgaggg ttctttctac gcagccacag tagaactcgt
     2101 acagatgcgg caaagccggc aagatctccc tggcccgcac cagatctatc tcgaaaaccg
     2161 ttgcgacggg ccggtctata cgcatatccg gggtgacgct ggctatcctg agagtgccgt
     2221 attttctggt gacttcggat ctgaaaagcc ttgaaagctc catgaccaca gatttatcga
     2281 tgtttctttc ggacttcggc agaccgaagg caatcttcaa aacgttttga accgggaaga
     2341 tgaagacctc gccggaaggg aactccagcc atctcctgta gttcttgatg cagatatcga
     2401 tgagctctcc gtctatgagt tccgggagcc ggaaatactc ctttcccctc agagagtcct
     2461 tcagaatctc cctcttcaga agtctctcgt ttccatagta agaaaggaag tagagcttta
     2521 ggggagtctg aaagatcgct ctctggaatg tcagttcggc atcaccgaag aaatccgaga
     2581 aagcctccct catctcttcg gcctcttcct ttccaaacct gtagagggca ctggcggttt
     2641 cgaatggaga ggagtttgaa agtccgcaga agcccctgcc ctcgctatct acgaaaatcg
     2701 atagaaccgg gaaatccctc ctcatcgtct gaacgaccag agtcatgaaa tgtccaaagg
     2761 cgttgtcgag cttgtcggaa cgctcgttca caaggcttcc gatctccatg aaccttccac
     2821 ttatgctgtt gtaaactctc tcaagcctgt ttctgtaact ctccggtcct gcatagtaaa
     2881 tcaactcaga aacaccaccg acgatttgcc ataaatggat ctggcttccg aagcgaaatc
     2941 ggaattgtgg ctcttcggaa gtacgaacaa cacccttctg gcaaccgacg ggagatccgt
     3001 atagccgtcg gtgaagatta tcaacccttc cggcctcagg ttttcctgtg agtaatctac
     3061 ggccggttga agatcggtct ctcccctgcc caccaacttg aggtctctcc acataccctt
     3121 gccatacttc atcacgctct ggacggtctc atcgacctgt accagccaga ttctggaatc
     3181 ggtgattcgg gtcaccgcat ctatttcacc gaagaaagcg ttgaattcgt cttctatgac
     3241 gctaccgctg gtatcgacca ccacaaccag cttggccgcg tagtcgcttt tccagcccgg
     3301 ttgatcatcg tagcgcctgt tcggtctcat aggggttctg tatctaccca cgtaggtcga
     3361 tgaaccgaaa aaccttcgta tcacgttcct ccagtcgagc acgggattgt cgagaaccag
     3421 tttcattacc tcttccagac cttcaggcat tcccgccctc gccttttcca gcgtttcgga
     3481 gacgacctgc ttcaaaagat cttcgataaa ttctttcggc agttcgaagc tgccaaaatc
     3541 ttcgtgagaa tccactctgg agagattacc ctcgagaagt tcgaggtcga tggatctatt
     3601 ttttctcata aagtccattg cccacgaatg gtagaactca gccgtctcgc ccgggtgggc
     3661 catgggcgga ccggcgaaga atctctcgtt gtcggtgcca cacccctccg aaagcagaga
     3721 gtccagagga agactgaagg cgtccagctc aggtatgtac tgattcaccg ccgcatccat
     3781 agagagatcc catacgactt tctctctgga cctgttcacg ggaatcaata tatgtccatt
     3841 cactatgtgg agacattcat gtttcaacag agcctttgta aaggccagag gtttgttgcc
     3901 cagaacgtcc gggttgtaga gtaactgaaa ccttcctctc gatgtcacgc gcagcctgac
     3961 ggttcttgtg gctgaggaag gtattctatc aacgaaaagg agcaagaagt tgtagaaagg
     4021 gctttccttc cccagttcaa aaacggcacg ttccatcagt tcttcatttt gcactctcat
     4081 ggctcccctg aaggaatttc atgacgtcgt gatccatcgc gagctcttcg aggagatttt
     4141 cgaagagagc acgcttcagg cctttttctc cttccatctg gtggactata tgcctgatga
     4201 tcgagaagaa agagtctctc gggatcactc tccctatttc gagaaggttt cccgcgatcc
     4261 tcggaaccat ctggaagtac tcctgaacct gcatttcgtc gagctccgaa agatgtctgg
     4321 ttattctcat gacggaggcg ctgatcccgg atatgtcact ctgggagatt ctcttcaaaa
     4381 tactgtcgtc cagttccatg agcagggttc tgggagaggg gaggagctgg ttgccctgta
     4441 tcttgctcat gaaagttctg gcggcttccg gtccgacgat tcccgcggcc agcacgtagc
     4501 cgtacttttc tatctcgtca ctttcgagga taccgaagac ccttccgagt ttgtaccagc
     4561 ttctcggact tggcctgagt tccagtctca tcgaaacgat tgtgtccctg gaaaggaatt
     4621 cggggtactc tccgatgaat ctggtgacac agtcgggaac gttttgagtt ttcgaccatc
     4681 tcagccattc ttcgggatcg ggagtcaatt ctatatgaaa gaaccttgac atgaaggccg
     4741 ggtcggttat gagttcaacc tggtcgtact cctcgtctgg cgggttggcg gcagcgatta
     4801 tccagctgtc ctcgggtaga aaatggttgt gtattcgctt gtcaatgaga agctgcatta
     4861 tggcgttcct tattgatctg tgggccctgt ttacctcg
//
LOCUS       DAOOLY010000194         4888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20829_length_4888_cov_3.891579, whole genome shotgun sequence.
ACCESSION   DAOOLY010000194 DAOOLY010000000
VERSION     DAOOLY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20829_length_4888_cov_3.891579"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLD15_09225"
     CDS             complement(<1..835)
                     /locus_tag="PLD15_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64131.1"
                     /translation="MRRILKKGIEHKKARWGWFFVLPSMAFFAVFSFYPIVNALYTSL
                     FRKRLLSLRPPTFIGLENYEYLLKSPDFWNSIKATFIFTLGTFIPILVFSLLLAAFIM
                     SRKRFQKFFQMAFYSPAVLSSVVAAAIWLLILDPRGLANMGANFLLGTKGVDYKWLAS
                     ANMVRFSTILVYFWKYIGYFTIIYITGLASIPQSLHEAAKIDGASGWKDFWHITIPLL
                     KPTTVLVSIMSMLQCLKTFSTQYLFTQSGAPLEPINVITLNIYNTAIRDHNIGRASAM
                     SI"
     gene            complement(903..2183)
                     /locus_tag="PLD15_09230"
     CDS             complement(903..2183)
                     /locus_tag="PLD15_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64132.1"
                     /translation="MNRRSFFVMFLCLVIPISLLVGKTIELQFWTHEDPNRTVIENRY
                     IEEFQKMYPDVVIKRVTQSSTKIQELVLTAFAANQGPDIFNMSIEDEYAYIVNQRVAS
                     VNYEAAGYENKEALINAYLPGTLDPVVFEGEIYGLPLEITNWCILLNKKVFKDAGLDP
                     EKDYPKTWEEMVEVSEKLTIREGQIITRRGFDFRYPYYLVAFIPMVEQLGGKLISDDG
                     KTAIVNDEAWLKFLDFMAAWGPNGLNLGSPTYKNARSLFNMDNNDIGMCTTGLYQLAR
                     IKADNPAFYDSREWMVVPFPQFENAVNYVPAHYYGHYYMVNVQKPKENQEMAWKFIAF
                     MLSHPEEYLDKVAIIQPTNELLESDTFKNYPYSDVFISDLEKSTVVYFSEYSAKIQSL
                     VKEAVESVMLSNASSRDALNALRRKVQEVLDDQY"
     gene            2383..3186
                     /locus_tag="PLD15_09235"
     CDS             2383..3186
                     /locus_tag="PLD15_09235"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HPB64133.1"
                     /translation="MKVTILITVLIVSTALLGSSFLVNITPLESDQPFQFQNQAGSRT
                     SSIGYTVISNTDSDSTIIVLGGWLFKPSGEEETLETSLEIYSENDSYSKKLTLVREGM
                     YYTLKPLLVSYPSGYSLKVMGIEAMISADGGEDMTVVNYLLVDLVAKDRLSAGIEIGL
                     EKEGKFVETPLTGIEPVVIIKAGSKPTGGYQLSIQNVKLSGDQIQVQAKLKSPGKNDY
                     VTQAFTYPFIAFRLLDLMVGEYRILVNMDVEVDSSIVKTEQFSGTFYVD"
     gene            complement(3174..4523)
                     /locus_tag="PLD15_09240"
     CDS             complement(3174..4523)
                     /locus_tag="PLD15_09240"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPB64134.1"
                     /translation="MRREGIHKANNISSKTGRFVLYWMQASQRAVDNDALDYACEISN
                     YLSLPLLVLFVLHGEYPSANRRSFTFMLEGLEETEKRLKERGITFLLKAGNPVDKVLE
                     IAKNASVLVSDVGYLRHQQFWRERIAHRVESPFFVVESNVIVPVGQVSRKEEFSAATI
                     RPKISRLLIDYLESRPVESRRLKGLGVEDFETVSLSACLKDLKFVDDVPAVSSFKGGT
                     SQAIEMLESFLRDKIDIFCRERNDPNSDALSNMSPYLHFGQISPSYIARKITERESPC
                     AEAYLEELIVRRELSMNFTLYNPDYDRFEGLPAWAKKTLNEHREDMREYIYSREQFES
                     RSTHDPYWNAAQNEMALTGKMHGYMRMYWGKKILEWSESPEAALDTAVYLNDKYELDG
                     RDPNGYAGIMWCLGKHDRAWAERKVTGKVRYMNSAGLRRKFDADGYVRRIDRLTQST"
     gene            complement(4540..>4888)
                     /locus_tag="PLD15_09245"
     CDS             complement(4540..>4888)
                     /locus_tag="PLD15_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HPB64135.1"
                     /translation="AGNLEKKDLDEVFDRYAPHFYRAMSTPPKHTSHINVLMHGLGYF
                     SRYLSSSEKKFFLDSLERYRQAKIPLSVPLHILKSYVVAFENEYLARQTFFEPYPEEL
                     VEISDSGKGRALK"
BASE COUNT         1230 a         1212 c         1143 g         1303 t
ORIGIN      
        1 tgatactcat cgcgctggct cttccgatat tgtgatctct gatcgccgtg ttgtagatat
       61 tgagagtgat aacgtttatc ggctccagcg gagcccccga ctgcgtgaac aggtactggg
      121 tactgaaagt cttcaggcac tgcaacatag acattatcga aaccagaact gttgtaggtt
      181 ttaaaagagg tatcgttata tgccagaagt ccttccaccc gcttgcgccg tctatctttg
      241 ccgcttcgtg cagactctgc gggatactcg ccaatcccgt gatgtatatt attgtgaaat
      301 aacctatata cttccagaag taaaccagta tcgtcgaaaa cctcaccatg ttagccgatg
      361 ccagccattt ataatcgacg cccttagttc ccagtagaaa attagcgccc atgttggcca
      421 gccctctagg atccaatatc agcaaccata tcgccgcggc aacaacggag gaaaggacgg
      481 ccggtgaata gaaagccatc tggaagaact tctggaatct cttcctggac attatgaagg
      541 ccgcaagcag aaggctgaag accagtatgg gtataaaagt tccaagtgtg aagatgaagg
      601 ttgccttgat cgagttccag aaatcgggag atttgagtag atattcgtaa ttctccagtc
      661 caatgaaagt agggggccgc agcgaaagca gcctttttct gaaaagactc gtatacaggg
      721 cgttcactat cggataaaag gaaaaaactg cgaagaaggc catcgacggt aacacaaaaa
      781 accatcccca ccgggccttc ttgtgctcta ttcctttctt gagaatgcgc ctcaagggtg
      841 aacccactcc tttcgcgaaa agaataaacg gcccgccaaa gcggcgggca cgaaatcaat
      901 catcaatact ggtcgtcgag gacttcctga acctttcttc tgagcgcgtt caacgcgtct
      961 cgagacgagg cgttgctcaa catgacagat tctacggcct cttttactag tgactgtatc
     1021 ttcgccgagt attcggagaa ataaacaacg gtcgatttct caaggtcgga gatgaatacg
     1081 tccgagtagg gataattctt gaaagtgtcc gattccagca attcattcgt cggctgtatt
     1141 atcgctactt tatcaaggta ttcttcggga tggctcaaca tgaaggcaat gaacttccat
     1201 gccatctcct ggttttcctt cggcttctga acattcacca tgtagtagtg gccgtagtaa
     1261 tgagccggca cgtaattcac ggcgttctcg aactgcggga aggggactac catccattcg
     1321 cggctatcgt agaaagctgg attgtcggcc ttgatccttg ccagctggta gagaccggtt
     1381 gtgcacattc ctatatcgtt gttgtccatg ttgaaaaggc ttctggcgtt cttgtaggtt
     1441 ggagatccca agttaagacc attcggtccc cacgcggcca tgaagtcgag gaacttcaac
     1501 caggcctcgt cattgacaat cgccgtttta ccgtcgtcgc ttatcagctt tccaccaagc
     1561 tgctccacca tggggatgaa agcgaccaga taatacggat acctgaaatc gaaacctctt
     1621 ctagttatta tttgaccttc tctgatggtt agtttctcgg acacttcgac catctcttcc
     1681 caggtcttcg gatagtcttt ttccgggtcg agtccggcat ctttgaagac cttcttgttc
     1741 aacaggatac accagttggt tatctccaga ggcagaccgt agatctctcc ctcaaagact
     1801 acagggtcaa gcgtgcctgg aaggtaagcg tttatcaggg cctctttgtt ttcgtatccg
     1861 gcagcttcat agttgaccga ggccacgcgc tggttaacta tataagcata ctcatcttcg
     1921 atggacatat tgaagatgtc cggaccctga ttcgccgcaa atgctgtgag aacaagctcc
     1981 tggatttttg tggaagattg tgtcactctc ttgataacca catcggggta cattttctgg
     2041 aactcttcga tgtagcggtt ttcgatcaca gttctgtttg gatcctcgtg agtccagaac
     2101 tgcaactcga tagtttttcc aacaagcaac gaaatcggaa tgacaagaca gagaaacatg
     2161 acaaagaaag aacgcctatt cataaaaaca cctcccgtaa agttggggta aagtaccccg
     2221 tatctctata atacaactct agcgatcaat tacgaaaagc gtttataggc ttttcgagtc
     2281 tttgggaggc ttttgaatac tacaaatgag gtgttatgat caattgctta aaccggctac
     2341 gacttcttgc agtcatatag tcaggatagg gaggtgtatc ttgtgaaggt tacaattttg
     2401 atcacggttc tgatcgtttc taccgcgctc ctcgggagca gttttctggt gaacattact
     2461 ccgctggaaa gcgatcaacc ctttcagttt cagaaccagg caggcagcag gacttccagc
     2521 atcggataca cagtgatttc caacaccgat tccgatagca cgattatcgt tcttggagga
     2581 tggctcttca aaccctcggg tgaggaagag acacttgaaa cctctctgga gatatattcg
     2641 gaaaacgatt cctattcgaa gaaactgacg ctcgtccggg agggaatgta ctacactctg
     2701 aaaccgctgc tcgtatcata tccctctgga tactcgttga aagttatggg gatcgaagcc
     2761 atgatctccg cagatggagg cgaagatatg acagtggtga attacctttt ggtcgatctg
     2821 gttgctaagg ataggctttc cgccggaatc gaaattggtc tggaaaagga agggaagttc
     2881 gtagaaacac cgctgaccgg aattgagcct gtcgtaataa tcaaagccgg ttcaaagccg
     2941 accggaggtt accagctatc gatacagaac gtaaaactct ccggcgacca gatacaggta
     3001 caggcaaagt tgaagagtcc cggcaaaaac gattacgtca cacaggcttt cacctatccg
     3061 tttatcgctt tcaggctact ggatttaatg gtcggagagt acagaatcct agtgaatatg
     3121 gatgtcgaag tcgattcttc gatagtgaaa actgagcagt tctcgggcac cttctacgtc
     3181 gattgagtga gtctgtctat tctcctgacg tatccgtcgg catcgaactt gcgccttaaa
     3241 ccggccgaat tcatgtaccg aactttaccg gtgacttttc tctcggccca tgccctgtcg
     3301 tgttttccaa ggcaccacat gatgccggcg taaccgttgg gatcgcgacc gtcaagttcg
     3361 tatttgtcgt ttagatatac agccgtgtca agtgccgctt ccggcgattc gctccactca
     3421 agtatctttt ttccccagta catgcgcatg tacccgtgca tctttccggt gagcgccatc
     3481 tcattttgag ccgcattcca gtaaggatcg tgcgtggagc gactctcgaa ttgttctcta
     3541 gagtaaatgt actccctcat atcttccctg tgttcgttca gtgtcttttt cgcccacgcc
     3601 ggcagacctt caaagcgatc gtagtcggga ttatacagag tgaaattcat actcagctcc
     3661 cgcctgacta ttagctcttc cagataggcc tctgcgcatg gcgattctct ttcggtgatc
     3721 ttcctggcaa tatatgaagg cgatatctga ccgaaatgaa ggtacggact catattcgat
     3781 aatgcatcgc tgttcgggtc gttcctctcc cggcagaaga tatcaatttt gtctctcaga
     3841 aagctctcca gcatctcgat agcctgcgaa gttccacctt tgaaactgga aaccgccgga
     3901 acatcgtcca cgaacttcag atctttcaaa caggccgata gagaaacagt ctcgaagtct
     3961 tctacaccaa gacccttcaa acgacgactt tcgaccggcc ggctttccag gtaatcaata
     4021 aggagacgac ttatcttcgg cctaatagtg gccgcactga attcttcttt tctgcttacc
     4081 tgtccaactg gcacgataac gttactctct acaacgaaaa agggggactc cactctatgg
     4141 gcgatcctct ctctccagaa ctgctggtgc ctgagatatc cgacatccga caccagaacc
     4201 gatgcgttct tagcaatttc cagaacctta tccacaggat ttccggcctt caagagaaag
     4261 gtgattcccc tttctttcag tcttttttcc gtttcttcca gaccctccag catgaaagtg
     4321 aagctcctcc ggttggccga agggtattcg ccgtgaagaa cgaataaaac gagaagcggt
     4381 aaagaaaggt agttcgagat ttcgcaggca taatcgagtg cgtcgttatc aaccgctctc
     4441 tgagaagcct gcatccagta gagcacgaat ctccccgttt tcgaagagat gttgtttgct
     4501 ttgtgaatgc cctctcttct catgccgacg tccggggctt catttcaaag ccctcccttt
     4561 ccccgagtcg cttatttcca caagctcttc ggggtacggt tcgaaaaagg tctgcctcgc
     4621 caggtactcg ttctcgaagg caactacgta cgatttcaat atatggagcg gaacgctcag
     4681 ggggatttta gcctggcggt atctttccag agagtccagg aagaatttct tctccgacga
     4741 tgaaagatat cgtgaaaagt agcccaatcc atgcatcaaa acattgatgt gagatgtgtg
     4801 tttcggcggc gttgacatcg cacggtagaa gtgtggcgcg tatcgatcga agacttcgtc
     4861 cagatctttt ttttcgagat ttccggct
//
LOCUS       DAOOLY010000195         4872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_20927_length_4872_cov_4.301225, whole genome shotgun sequence.
ACCESSION   DAOOLY010000195 DAOOLY010000000
VERSION     DAOOLY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20927_length_4872_cov_4.301225"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLD15_09250"
     CDS             complement(<1..782)
                     /locus_tag="PLD15_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64136.1"
                     /translation="MKKFLVVTLMIFSTLLSATVSFINPVGPTLVPIAQLLSSNVSAE
                     IPGLEINLWRSVDEAISLIVGKKAQISLLPVTIGAKLASSGIDIKLAAVSMWNGFYFI
                     TVEKAISSVEDLAGMEIFTLQAPGQTADIILKGAVEKSGLKIGKDIKIAYVGGAEAVQ
                     LLASGKTKAILVPEPFATLAMTRVEKAVRSMPIEELWASFNEEKINIPTSGLFVSGDL
                     DRSVVEAFLMLYGQSMSLSLTDRTGTASVVSEKMGGFPIPVL"
     gene            998..1270
                     /locus_tag="PLD15_09255"
     CDS             998..1270
                     /locus_tag="PLD15_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB64137.1"
                     /translation="MNKKELIAEIAERTGVTKKDAGKTLDTVIEIVEKTLSKGEVVRL
                     VGFGTFMVASRKARKGVNPRTKKPITIPGGKVPKFVPGKELKEKVK"
     gene            1331..2212
                     /locus_tag="PLD15_09260"
     CDS             1331..2212
                     /locus_tag="PLD15_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPB64138.1"
                     /translation="MKTVLIVSLVAALTVISVITLIYTVGLRNEILSSTNNIGTKNLE
                     IELKALSAEIGKVKDAVESLNIVDYSPQILSFKEDFTSLQTVLDKLNITNSQEYSTLN
                     ETLDSLEESVNELHLLLGENKEVPENQYKIEELAGKIELMESEFLKNVETIKVELISK
                     LDSIRLPEIPTVPTYDATEGSGIRIRIESGFKGSSILHDSDLLVVMNELYSLKATSIS
                     LNGKRILPYTYIRCVGSTVIIDGEPTTISPIVIEVLGDYDYLVSGLGLLSEAFKGREI
                     GMTFLPLEFITIPAGGG"
     gene            2218..4011
                     /locus_tag="PLD15_09265"
     CDS             2218..4011
                     /locus_tag="PLD15_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPB64139.1"
                     /translation="MKKIILLVIASASLLFFAGCDSIFITRDDFLLRMDALEKKIDSL
                     ATEQRRIVQLEDAIEELTFRFNFVQAIEPASIGYEEIEQLKYELRLIKNLLAESVIDS
                     ASSAAIVRTLYDKIDGVLGSSVQSNIVTKEIDDLNRKVQALETLNYSRYTELVERISE
                     INTYDSSIDDSKYLELLARLDEIEIQPPVINIQEPDDGRYEELMAKIEELASRSDNTE
                     VDNAKYESLLTRLGELERTVGSSAIDRELFNELVSRIDEMDARTRSLAENVQSESSDD
                     YVSMSTFLGEISDIRSRLGAAAYETIEPVSYISYIVKSGDNLSSIAKAYGITMDQLKE
                     MNPEISNWNLIYRGDEIKIPLSLDNLMAKASLASHFGLNLGIEFLVDSIESNFGSYDY
                     GYANPGIDLTVPVNSRITAFLPGKVILSERVNDLYGEMVVVDHGNNIKTVYARLGSRT
                     VSKGDFVRVGDTIGIASEIKGNLHFEFWKSDVPVNPADIIFDNAGTFQVTMYTEWDDG
                     KNPTSPSFKMTASGNYVKGYRTVATDPTVIPTGSIVYIPFFSSSPNKGFFVVEDTGSS
                     IKGNKLDVYTHDFNIASNFKEELLVYVVKKP"
     gene            4041..4856
                     /locus_tag="PLD15_09270"
     CDS             4041..4856
                     /locus_tag="PLD15_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64140.1"
                     /translation="MIVSIASGSVVIGQRYPLGIMPSGIVRVENGYGTYYVTLVKGDS
                     EMIVFDSSFKPVTIMDAVKNDGVNDIIYRDGKLYCFGFFSGRLVVIDASGYPGRWKKI
                     DEIPTSSRLITGAFIDGRLGILSNENELIVLDTGNRTILKRYKLPVTALTIAEDSSRF
                     YVSLFYNYNTLNRSMETEKGLMVFDIDGQLSVSLDIGKRPSYISIDGDRLFLVSYMEE
                     NLKILNKNTLKEIKTIELGRYPNYPVMQNGKLWISIVASNVWEPGVYGWEIYS"
BASE COUNT         1343 a         1150 c         1234 g         1145 t
ORIGIN      
        1 tccagtaccg gtatcgggaa gccgcccatc ttttcggaaa cgaccgaagc agttcccgtt
       61 ctatctgtca gagagaggct catcgattga ccgtagagca tcaagaacgc ttctacgacg
      121 cttctatcta gatcgccgct gacgaaaagc cccgaggtcg ggatgttgat tttctcttcg
      181 ttgaaggagg cccatagttc ttcgatgggc atcgatctaa cggctttttc gactctggtc
      241 atcgcaagag tagcgaaggg ttctggcaca agaattgctt tagtctttcc ggaggccagg
      301 agctggacgg cctcggcacc gccgacgtat gcgatcttta tatcttttcc gattttgaga
      361 ccgctcttct cgacggcccc tttcaggata atatcggccg tctgcccggg ggcctgaagt
      421 gtgaagattt ccataccggc cagatcctcg acgctggaaa tcgccttttc tacggttatg
      481 aagtagaaac cgttccacat actgacggca gccagtttaa tgtctatacc actcgaagcg
      541 agtttcgccc cgatggtgac aggaagaaga ctgatctgag ccttttttcc gacgatcaat
      601 gaaatagctt cgtcaactga acgccagagg tttatctcga ggccggggat ttctgccgaa
      661 acgttagaag aaagaagctg ggcgatcgga accagcgttg gtccgacggg atttatgaag
      721 cttactgtgg ccgacaaaag agtcgaaaaa atcataagag taactaccag aaatttcttc
      781 acgaataacc acctccatca aaattataca ctgacttcgg atatgtagcg agggcttttc
      841 agctttccac gccatataaa gagactgaaa gccattattt ggttctcaga gtgaaatccc
      901 ggagatagcg aatgaaatcg acctctgaaa gggatttggt aattgagccg cggagtggta
      961 gaataatagc gacatctcat gcgaaggggg gaactgtatg aacaaaaagg aactcatcgc
     1021 tgaaatcgca gagagaacag gcgtaaccaa gaaagatgcc ggcaagaccc tggacaccgt
     1081 aatcgaaata gtcgaaaaga ccctgtccaa gggcgaagtt gtcagactcg taggtttcgg
     1141 tacgttcatg gtggcttcca gaaaggcaag aaagggagtc aacccgagga cgaagaaacc
     1201 tatcaccatc cccggcggaa aggtaccgaa gttcgtacct ggcaaggaac tgaaagagaa
     1261 ggtaaaatag tcttcaaggc tgagcggcgg ctctcaagcc gccgcttttg ttttggaggt
     1321 gaagttttta atgaagaccg tcttgatcgt ttcgctggtg gccgctctaa ctgtcatttc
     1381 ggtaataact ctcatataca ctgtcggcct gaggaacgag atcctttcca gcacaaacaa
     1441 tatcggaacg aaaaacctgg aaatcgaact gaaggcgctc tccgctgaaa tcgggaaagt
     1501 gaaagacgcg gttgaatctc tcaatatcgt tgattacagc cctcagatac tctctttcaa
     1561 ggaggatttt acaagcctcc aaacggtcct cgacaaactc aatattacca attctcaaga
     1621 atactcgacg ctgaatgaaa cactggatag tctcgaagag tccgtaaatg aattacactt
     1681 gcttttgggc gagaataagg aagtcccgga aaatcaatat aaaatagaag aactggccgg
     1741 gaagatagag cttatggagt cggaattcct caagaacgtt gagacaatta aggtcgaatt
     1801 gatttcgaag cttgactcca taagactccc ggaaatcccg actgtaccca cgtacgacgc
     1861 gaccgagggc agcggtataa ggatcagaat cgaatctggt ttcaagggga gcagtatact
     1921 gcacgacagc gatctactgg tagtgatgaa cgaactttac tcgcttaagg caacgagcat
     1981 ctccttgaac ggaaagagaa ttttgcctta tacatatata agatgtgtag gctcaactgt
     2041 tataatcgat ggtgaaccta cgactatttc acccatcgtg atagaggttc tgggtgatta
     2101 cgattatctg gtatccggtc tgggcctttt gagcgaggct ttcaagggga gagagatagg
     2161 tatgactttc ttgcccctcg agtttataac cattcccgcg ggagggggtt gattcaggtg
     2221 aaaaagatca tccttctggt aatagcttcg gcgagcctgc ttttcttcgc aggctgtgat
     2281 tctattttca taacccggga tgattttctt ctcaggatgg acgccctgga gaagaagata
     2341 gattccctgg ccacggaaca gcggcgaata gttcaactgg aggacgcgat cgaagaactg
     2401 actttccgtt ttaatttcgt ccaggcgata gagccggcct ctattgggta tgaagagata
     2461 gaacagctca agtacgaact ccggctaatc aagaatctgc tggcagaatc ggttatagat
     2521 tctgcctctt cggctgccat agtgagaacg ctttacgaca agatcgacgg cgtcctcggc
     2581 tcttcggtac agagcaacat agtcactaaa gagatcgacg atctgaacag gaaagtgcag
     2641 gctctggaga ctctcaatta ctccagatac accgaactgg tcgagagaat ctccgagatc
     2701 aacacctacg acagctccat agacgactcc aagtacctgg aacttctggc caggctcgac
     2761 gagatcgaaa tccagccgcc tgtgataaat attcaggaac ccgacgacgg caggtacgaa
     2821 gaattgatgg cgaagatcga ggaactggcc tccagatccg acaacaccga ggtcgataac
     2881 gcaaaatacg aatcgcttct cacaaggctg ggcgagctcg aaagaaccgt gggatcctcc
     2941 gccatagaca gggagctttt caacgagctg gtctcgcgca tagatgagat ggacgcaagg
     3001 accaggagtc tggccgaaaa cgttcagtcg gagagctcgg acgactatgt ttcgatgagc
     3061 acttttctcg gcgagatctc cgacataagg agcagactcg gggcggcggc ttacgaaacc
     3121 atagaacccg tctcttatat ttcctatata gtgaaatccg gcgacaacct atccagtatc
     3181 gcgaaggctt atggaataac aatggatcag ctgaaagaga tgaacccgga aataagcaac
     3241 tggaacctta tatacagggg cgatgagatc aaaatacctc tctcgctgga caaccttatg
     3301 gccaaagcct ctctggcttc acacttcggg ctcaatctgg gaatcgagtt tcttgtcgat
     3361 tctatcgaat ccaacttcgg aagctacgat tacggctatg ccaaccccgg tatagacctc
     3421 acggtgcctg tcaattcgag aatcacggca tttctgcccg gcaaagtaat actctcggag
     3481 agggtcaacg acctgtacgg agaaatggtc gtggtggacc acgggaacaa catcaagacg
     3541 gtctatgcgc gtctgggaag cagaaccgta tcgaaagggg attttgtaag ggtgggcgac
     3601 acgatcggaa tcgcgagcga aatcaaggga aacctgcact tcgaattctg gaaatccgac
     3661 gttcccgtca acccggccga catcatcttc gacaacgccg gtactttcca ggtcaccatg
     3721 tacaccgaat gggacgacgg caagaatccc acctctccgt ctttcaagat gaccgccagc
     3781 ggcaattacg tgaagggtta caggaccgtc gccacagacc cgactgtcat tccgaccggt
     3841 tccatagtgt atataccgtt cttctcgtca tctcccaaca aaggcttctt cgttgtcgag
     3901 gatactggaa gttctattaa aggcaacaaa ctggacgttt atactcacga cttcaacata
     3961 gcctcgaatt tcaaagagga attgctggtt tacgtggtga agaagccttg aagagattat
     4021 tctttatttc gttctgtttg gtgattgtat ctattgcatc cggttcggtc gtaatcggcc
     4081 agaggtaccc gctgggcata atgccctctg gaatcgtgag ggttgaaaac ggttacggta
     4141 cctactacgt caccctggtc aagggcgatt cggagatgat agtcttcgat tcctctttca
     4201 aaccggtaac tattatggac gcggttaaga acgatggtgt gaacgatatt atctacaggg
     4261 acgggaaact gtactgtttt ggcttcttca gcggcagact cgtggtcata gacgcctcag
     4321 gttaccccgg caggtggaaa aagatagacg agatacctac ttccagcagg ctcataaccg
     4381 gcgctttcat cgatggaagg ctcggcatac tctcgaacga aaatgagttg atagttctcg
     4441 ataccggcaa cagaacgatt ttgaagcgtt ataaactgcc ggtgacagcg ctcacgattg
     4501 ccgaggattc cagtcgcttc tatgtctctc tcttctacaa ctacaacacg ttgaaccgct
     4561 ctatggagac ggaaaagggt ttgatggtct tcgatatcga cggtcagctc tcggtcagtc
     4621 tcgatatcgg caagagacct tcctacatct ctatcgatgg ggatagactg tttctggtct
     4681 cgtacatgga agagaatctg aaaatcctca acaaaaacac cctcaaggaa ataaagacga
     4741 tcgagctcgg aagatatccc aactacccgg tcatgcaaaa cggcaagcta tggatttcca
     4801 ttgttgcgag caatgtctgg gagcctggtg tgtacggctg ggagatatac agttaggagg
     4861 ggataacttg aa
//
LOCUS       DAOOLY010000196         4857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21009_length_4857_cov_5.048521, whole genome shotgun sequence.
ACCESSION   DAOOLY010000196 DAOOLY010000000
VERSION     DAOOLY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21009_length_4857_cov_5.048521"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLD15_09275"
     CDS             complement(<1..355)
                     /locus_tag="PLD15_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64141.1"
                     /translation="MSLPAGRPDNKERMASTNEVAGWFSAKARTAGGIDASGNLLDQN
                     GEITVDTRNVACYRLTIEAEWEFAAREGFRRTWLGGSKEQRNWLTVESFSVHRGEFRS
                     NSIKIPTRSFVGMTES"
     gene            951..2786
                     /locus_tag="PLD15_09280"
     CDS             951..2786
                     /locus_tag="PLD15_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB64142.1"
                     /translation="MKRTIRLKMITIFIFLFVVLGTIAIWSITNFTRLGSAIEAIMEA
                     NYQSVVSAQSMTLALERQDSAELASMFSPEDSTADIFVYNQNEFLKALSRAEDNITEP
                     EEPAILSAIGELYVEYVNRYYVLSDIRRESTMEEASSYYYNNILPLFEEVKQLTRDLL
                     TVNQEEMLAKRNRALEVARNASFSTAIISSVAILLGVLFSMYLVVKIVRPITLLIEKI
                     KLIAQGDYKQRLPGKGNDEIAELSKEFNTMAARLNSYEEMNVQKLMEEKQKAEGIVDS
                     IGDVVLVTDSENRLIVLNRAAEKIFDIRKDEVLRNHFLEVIKNEEIFEQVKRTLGGEK
                     NEEKNYVDVNLEKDGEKKYFRVKSKPIKNEQREIIGVVTLMQDITKLKEVDSMKSEFV
                     STVSHEFRTPLTSMGMAVNLMLDGTTGDINEEQRELLQALKEDQERLNSLVSDLLDLS
                     RIESGKIQMDIQPNSLYEIVENALSPFHIQLKENDVKLKIDVSEDLPKVKADFNKISW
                     VIANLVGNALRYVPKDGTGKIEISAKKTMNKVLVSVSDNGTGIPEDMQEKIFEKFVKA
                     NDSQGGTGLGLAISREIMLAHGGEIWVSSKQGAGSTFYFTLYFSG"
     gene            2797..3153
                     /locus_tag="PLD15_09285"
     CDS             2797..3153
                     /locus_tag="PLD15_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB64143.1"
                     /translation="MTILIVEDEKNIVLSLKLMLEKMGHDVVCARSGVEALEISRKIS
                     PDLVFLDIVLPGMDGFLVCQAMRKESDTSKTPIIFISALSREEDIKKAFNAGASEYIV
                     KPFTPEKIRETVEKWL"
     gene            3167..3829
                     /locus_tag="PLD15_09290"
     CDS             3167..3829
                     /locus_tag="PLD15_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB64144.1"
                     /translation="MKKILIVDDAQNIRVTLKRCLSSEDNELDVAMNGEEAFAKIMEN
                     DYDLVFLDLQLPAMSGIEVLRKLRYEGNHVNVVIITAYGTVENAVEAMKLGAIDFVQK
                     PFSPGEIRRVVSAVWARNSISESQLKSFEDFLEFSKQKIMEGDYEKAESSLGKALTLN
                     PNDAEANYYQGLLFENKGEIEKAKDSYRKVLKLQPSYSQAEENLNHLLSAGRKLRRDE
                     EE"
     gene            3816..4253
                     /locus_tag="PLD15_09295"
     CDS             3816..4253
                     /locus_tag="PLD15_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPB64145.1"
                     /translation="MKKNKPSKYVIIIGCGGLGSSIAGQLSAAGNNVVVVDRDREAFK
                     KLPPEFSGFTIMTEVIGKSALVDSKIEQAHILIASTDDDNTNIMIAQIAKMIYHVPKV
                     IARVFEPSRMRVYKDLDIETISPTILAASVVSDSILNEGDVES"
     gene            4250..>4857
                     /locus_tag="PLD15_09300"
     CDS             4250..>4857
                     /locus_tag="PLD15_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPB64146.1"
                     /translation="MKIIVVGGGMKAYFLAKNLISMGYSVTMINKNKEDALQLAKIDR
                     ATVVHGDATLPEILEDAGTHTADVVVALTSSDPDNLVICQTAARLFGVRKTVAIVNDP
                     GNVEVFKTLGIDYVVSTVRTISSLIQQSVGNETIMNLTPVEEGKVMILEVDVNTEDPI
                     SGKQLNLFEMPHEAIIGCIIREGKPIIPRGDTTVSPGDRLIIL"
BASE COUNT         1384 a         1042 c         1251 g         1180 t
ORIGIN      
        1 tagattccgt cattccgaca aagctcctgg tcggaatctt gatgctattg cttcgaaatt
       61 ctcctcggtg aacggagaac gattcaacgg tcaaccagtt cctttgctct ttcgatcccc
      121 ccaaccacgt tcttcgaaag ccttcgcggg cagcgaactc ccactcggcc tctatggtaa
      181 gcctgtagca agccacattt cttgtatcaa ccgtgatttc cccattctgg tcaagcagat
      241 tgccactcgc atcgatgccg ccggctgttc gtgcctttgc cgaaaaccag ccggcgactt
      301 catttgtgga agccatccgt tccttattgt ctggccggcc cgctggcaat gacatatgga
      361 ctgctcttgc ctggtgcgtc ggaatcttgg ttgttgcata tgtattcgca atgaaggcat
      421 ataagaagcg tgtgtagatg cccgtttcgc gaagaagata ctaagatcgt ccctccaaat
      481 caagaacccg gcttcttcga acgacggtat atggatttca ttactctaca gcgaaccgac
      541 aaacaagagc caggagcatt ctcttcaatg agcccacaga gctttagatt gacgctttcc
      601 cgcatagcgt tattttcaaa gatcaatcat aaacccagtt gcttgtttcc gcacagctta
      661 taaagaatct gccaattctt tcaaaacctc acctataacc acgccaagtc tagaaccggg
      721 cttcagtacc gaacggcttt ctggcggggt gctgatggct ttcagggaac attctagcaa
      781 aggctaagta agcattcaca ccctttctgc cttgtcaaat agcagaaaga ctttgggatg
      841 aatttcaatt ggaggtttcg gtattgggcc cggctttctg gcctgcttcc ttcattttca
      901 tggacaagcg ccttgggatg ggtctataat gatgattgtg aggtgagagt gtgaagagaa
      961 ctattagact gaagatgatc acgatcttta tttttctctt cgtagtactc gggactatag
     1021 ctatctggtc aatcacgaat ttcaccagat tgggttctgc tattgaagct atcatggagg
     1081 caaattatca gagtgttgtg tctgctcaga gcatgacgtt agcgctggag aggcaggaca
     1141 gcgccgaact ggcaagcatg ttttcccctg aggattcaac ggccgatatt tttgtttaca
     1201 atcagaatga atttttgaag gctctttcca gagcagagga caatattaca gagcctgaag
     1261 agccggcgat actgagtgct ataggcgagc tatatgtgga atatgtgaat agatattacg
     1321 tgctctcaga catcagaaga gagagcacca tggaagaggc cagttcttac tactacaaca
     1381 acatcctccc tctatttgaa gaggttaagc agcttacacg cgatttactc accgtgaacc
     1441 aggaagagat gctggcgaag cgaaacaggg cacttgaggt tgcccggaac gcttcctttt
     1501 ccacggcaat tatctcttca gtagcgatcc ttcttggagt gctcttttcg atgtacctgg
     1561 tcgtcaagat tgtcaggcct atcacccttc taatagagaa gatcaagctt attgctcaag
     1621 gcgactacaa acagcgcctg cctgggaagg gaaacgacga gatcgcggaa ctttcaaaag
     1681 agttcaacac gatggctgca aggctgaatt catatgaaga gatgaacgtg cagaagctta
     1741 tggaagagaa gcagaaggcc gagggcatag ttgacagcat cggagatgtt gttctggtaa
     1801 ccgacagcga gaataggctt attgttctca acagggccgc cgaaaagatc ttcgacattc
     1861 gaaaagatga ggtccttaga aatcacttcc tggaggtaat caagaacgaa gaaatatttg
     1921 agcaagtaaa gaggaccctt ggcggggaaa agaacgaaga aaagaactac gtcgatgtga
     1981 acctggagaa ggacggggaa aagaaatact tcagggtgaa atccaaaccc attaagaacg
     2041 agcagaggga gataataggt gttgtgactc taatgcagga tatcacgaag ctcaaagaag
     2101 tcgattcgat gaagtcggaa tttgtttcta ccgtttccca tgaattccgg actccgctga
     2161 cttcgatggg tatggccgtc aatctgatgc tcgatggcac aactggcgac ataaacgaag
     2221 aacagagaga attgcttcaa gctctcaaag aggatcagga gagacttaac tctctggtta
     2281 gcgaccttct tgacctttcg cggatcgaat ctggaaagat ccagatggat attcagccca
     2341 actccctcta tgagattgtc gaaaatgcat tgagcccatt tcacatacaa cttaaggaaa
     2401 acgatgtgaa gctgaagata gacgtaagtg aagatctccc gaaggtgaaa gcggacttca
     2461 acaagatatc ctgggtgata gcaaaccttg taggaaatgc cctgagatat gtgcccaaag
     2521 atggaactgg aaagatcgag atttccgcga aaaaaactat gaataaagtc cttgtctctg
     2581 tttcggataa tggtacgggc atccccgaag atatgcagga gaagatattc gagaaatttg
     2641 ttaaggcaaa tgatagtcag ggaggaacgg gacttggcct tgcaatcagc cgagaaatca
     2701 tgcttgcaca tgggggcgag atttgggtaa gcagcaaaca gggcgcgggc agcacctttt
     2761 acttcaccct gtatttttct ggttagaggt gcacgagtga cgattcttat agttgaagat
     2821 gaaaagaaca ttgtcctatc actgaagctt atgctggaga aaatggggca cgatgtggtt
     2881 tgcgctcgca gcggtgtcga ggcgctcgag atttcgagga agatttcgcc cgatcttgtg
     2941 tttctggaca tagtcctgcc cggtatggac gggtttctcg tctgtcaggc gatgagaaag
     3001 gaatccgaca cttcgaagac accaataatc tttattagtg ctttgtccag agaagaagac
     3061 ataaagaaag ccttcaatgc cggggcaagc gaatacattg tgaaaccctt tacaccagag
     3121 aagattcggg agactgtcga gaaatggctt tgaggaggta atgattatga agaagatact
     3181 tatcgttgac gatgctcaga acataagagt aactctaaag aggtgtcttt caagcgagga
     3241 caacgagctt gacgtggcaa tgaacggaga agaggccttc gcaaaaatta tggagaacga
     3301 ttatgatctt gtcttcctgg atctccagct gccagcgatg agtggcatcg aggtcctcag
     3361 gaagcttagg tatgaaggca accacgtaaa cgtcgtgatt atcacggctt atggtacagt
     3421 cgaaaacgca gtggaagcga tgaaactcgg agcgatcgat ttcgttcaga aaccattttc
     3481 ccccggtgaa ataagaagag tggtgtcggc cgtatgggcg aggaattcga tctctgaaag
     3541 tcagctcaag agtttcgagg attttcttga gttttcaaag cagaagataa tggagggaga
     3601 ttatgaaaag gccgaaagtt ctctcggcaa ggcgttgacg ctgaatccaa acgatgccga
     3661 ggccaattat tatcagggac tgctgtttga gaacaaggga gagatagaaa aggcgaagga
     3721 tagctatcgg aaggttctga agttgcagcc gtcttacagc caagcggagg agaatctcaa
     3781 tcatcttctc agcgctggca gaaaactacg aagagatgaa gaagaataaa ccttcgaagt
     3841 acgtgataat catcggctgc ggcggactgg gatcaagcat cgccggccag ctttctgctg
     3901 ccgggaataa cgttgttgtt gtggacaggg atcgagaggc tttcaagaag cttccccctg
     3961 agtttagtgg cttcacgata atgacggaag tcattgggaa gagcgccctc gtggactcaa
     4021 agatagaaca ggctcatata ctcatcgcat cgactgacga tgacaacaca aatatcatga
     4081 ttgcacagat tgccaagatg atttatcacg ttcccaaggt aattgcaagg gtcttcgaac
     4141 cctccagaat gagagtctat aaagatctgg atatcgaaac aatctccccg acaattctgg
     4201 cagcatctgt agtgagcgac agtattttga atgagggaga tgttgagagt tgaagataat
     4261 cgttgtaggc ggggggatga aagcttattt ccttgccaaa aacctgattt ccatgggcta
     4321 ttccgtcacc atgatcaata aaaacaagga agatgccttg cagcttgcga aaattgacag
     4381 ggcaacggta gttcacggtg atgcaacgct gccggaaata ctcgaagatg ccggcaccca
     4441 tacggcagat gtagttgtgg cacttacttc aagcgacccc gacaatcttg tgatttgcca
     4501 gaccgcggcg cgtcttttcg gcgtgaggaa gaccgtggcg atagtcaatg atccgggcaa
     4561 tgtagaggtc ttcaaaactc ttggcataga ttatgtggtt tccacggtaa ggactatctc
     4621 ttcgcttatc cagcaaagcg tcgggaatga gacaataatg aatctgaccc ctgtcgaaga
     4681 gggaaaggtc atgatactgg aggttgacgt gaataccgaa gacccgattt cgggaaaaca
     4741 actcaatctc ttcgagatgc ctcatgaagc aattatcggg tgcataatca gggaaggcaa
     4801 gcccataata ccgcgcggcg atacgacggt ctctccgggc gacaggctga taattct
//
LOCUS       DAOOLY010000197         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21064_length_4846_cov_4.507201, whole genome shotgun sequence.
ACCESSION   DAOOLY010000197 DAOOLY010000000
VERSION     DAOOLY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21064_length_4846_cov_4.507201"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PLD15_09305"
     CDS             <1..1026
                     /locus_tag="PLD15_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64147.1"
                     /translation="TRTLSFRKLLLSLLLVLCILSVWALIIWFGGESPFKGFRYIIEG
                     SLGNHYQFLSTLNKMVPLILAAAAVAVPAWTGMWNIGGEGQLLLGAFGAALVGFSIDF
                     GVGLVNIVVALIVSAAFGMAWAAWPAFFKIKLRMDEVVTTLMGNYIAAQLIAYLINNP
                     FRDPRSPLAQTPYIKSSFVIPYLFEGSQFSATFFVAIGMVLVLFIFRKRFITGYEFDI
                     TGSNPVFAGLSGIRVKKTKLLSMLIGGALAGLAGGLLVLGMTQRVMQSFSPGYGFTGL
                     LICLLAFNDPLLIVGIAFLFSALQTGSVNLQLFTSVPAEISGVLQAVMVLFVAAFKTF
                     IVGRREG"
     gene            1029..1928
                     /locus_tag="PLD15_09310"
     CDS             1029..1928
                     /locus_tag="PLD15_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64148.1"
                     /translation="MDIELITTLFRTSIRMATPILLASLGGALTYHAGIINVAMEGLM
                     LISAFFSVVFSYLTGSAFYGIIAGVVASMLISLLYSVFVSFLKANNFAIGFAINILIS
                     SLTLFLMRVMFQGKNAFNSPDIASIMELKVKTGNYFLDNYLLNFSILTYLSIAIAFLL
                     AYIIYKTSFGLRLRVCGEMPQALTAAGIKVGVIQLWASILCGGLCGLAGAQLSLYNVR
                     MFSRDMTGGRGFVALAAILISGGKPKRVLFISLLFGLFDALSVKLQNEKIAPQFALML
                     PYIMSVVVLLLFHLKTRKQYGKA"
     gene            2056..3819
                     /locus_tag="PLD15_09315"
     CDS             2056..3819
                     /locus_tag="PLD15_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPB64149.1"
                     /translation="MKRNSVRIFFLLLVVFLPLSVAMARGVTFLVNPSYSPFSYEENG
                     ELKGLAVELVKLLSSEFGLEAEMTSMPFEEAYAKLQDGGEYALPTLVFTPQRKSQFNW
                     VGPIAITPTYLYARDTSPDIRSIDDAKKAGKVGAVRGYYSQQLLAEQGLDTVIFDDEA
                     ALLKSLLEGDIDLAPFNFDVYTRLTEKIPESSKLRATIVLDLDMTYIGFSKDVPTDTL
                     RRWQEGLDGLKSSGDFAGLYEKWLPGRPVPGVYTLLTEEYPPVTYMGDGGPAGFVTEI
                     VRELMERNGMKEEIFLVPWAIGYELASNLPNVLLFSMDQTELRKPLFQWIGPVGRNTA
                     YLYGRADSAIAPADLDEARKVGTIATTKDWWTEQLLRDLGFTNLKSFLDPLDTVRQLM
                     NGEADLAIFTDLTVSELVQNAGYQMKDLNRLLEIQTNYFYVAASSGTDPEMVLILQKT
                     LDSMKRDGSFEEILRKYVPNVLITPLLLQSSTPATVRLQSVVNSLQIGELASLTLEEN
                     GSTGYIWDVRVSNPNVLSLAFVKHGVSDAGEESLTGASYEVEWTFRAERPGECVIVFS
                     LRRPWESVHPVKTFAINVKVE"
     gene            complement(3916..>4846)
                     /locus_tag="PLD15_09320"
     CDS             complement(3916..>4846)
                     /locus_tag="PLD15_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB64150.1"
                     /translation="TKQYTVVKGDTLWSIARRYGVDWTEIASFNGIENEYSLKVGTIL
                     KIPHSFSEQTVLITNGDHEIPAVVCMPDGDGPFPAVVMLHGTGSNKDEAGGGYLLTAP
                     ALAKAGIASIRFDFIGNGDSEADYINYNFMSAVSDSNAAFDYMAGLNGIDPNRIGVMG
                     WSQGGTIAMLAAGRNRSFRSVVCWAGAPDLKSIGTAENYEIAKANGYYELTFDWRPSL
                     KLGLQWFEEVYNTDVLEVFSNSFAPVLAINGAMDTVVDPVNAQRIVDASSNKNSRVLL
                     IEGADHTFNIFTGDMSAFNELIAATVDWFADTL"
BASE COUNT         1048 a         1288 c         1305 g         1205 t
ORIGIN      
        1 actcgcactc ttagtttcag gaagttgctt ctctcgcttc tgctggtgtt gtgcatatta
       61 tccgtctggg cattgataat ctggttcggc ggcgaatcgc ctttcaaggg attccgctac
      121 atcatcgaag gctctctggg caatcattac cagtttctct ccacactcaa caagatggtg
      181 ccgttgattc ttgccgcggc cgccgttgca gtccccgcct ggacgggtat gtggaacatc
      241 ggcggggagg gccagttgct tctgggagct ttcggagcgg cgcttgtcgg tttttcgatc
      301 gattttgggg tcggtctagt gaacatcgtc gtcgctttga ttgtttctgc cgcatttgga
      361 atggcgtggg cggcatggcc ggccttcttc aaaataaagc tccggatgga cgaggtcgtc
      421 accacgctta tgggtaatta tatcgcggcg cagttgatag catacctgat caacaaccct
      481 ttcagagatc cgcgctcgcc tctggcacag actccatata tcaagtcgag ttttgtgatt
      541 ccatacctgt tcgaaggctc tcaattctcc gcgactttct tcgtggccat cggtatggtt
      601 ctggttctct tcatattccg aaagaggttc attacgggct acgagttcga tatcaccggc
      661 agtaacccgg tttttgccgg gctgagtgga ataagggtaa agaaaacgaa gttattgtcg
      721 atgcttatcg gaggggctct cgcgggtctt gccggcggcc tcctcgtcct tggaatgact
      781 caaagagtga tgcagagctt ttcgcccgga tacggtttca ccggtctgct catctgtctt
      841 cttgccttta acgaccccct gctcattgtg gggatcgcct ttcttttctc ggctcttcaa
      901 acgggtagcg tcaatctcca gttgttcacg tccgttccgg ccgagataag cggtgtcctt
      961 caagctgtta tggtgctttt tgtggcggcg ttcaagacgt tcattgtcgg gcgaagggaa
     1021 ggttgaaaat ggatatagag ctgattacca cactattcag aacatctatc aggatggcaa
     1081 cccccatact tctcgcctcg cttgggggag cgctcactta tcacgcgggg ataataaacg
     1141 tggctatgga aggtctcatg ctcataagcg cgttcttctc ggtggtgttc tcatacctaa
     1201 ccggcagcgc cttttacggt atcattgccg gggtggtcgc ttcgatgcta atatcgcttc
     1261 tgtattcggt attcgtaagc ttccttaagg caaacaactt cgccataggc ttcgcgataa
     1321 acatacttat aagttcgcta accctctttc taatgagagt gatgttccag ggaaagaacg
     1381 ccttcaactc ccccgacatc gcctctataa tggagctcaa ggtaaagacg ggaaactact
     1441 tccttgacaa ctacctgctt aacttcagca ttctcaccta tctctctatc gcgatcgcct
     1501 tcctgctggc atacattatc tacaagacct ctttcggact gaggctcaga gtctgcggcg
     1561 agatgcccca ggccctcacc gctgccggta taaaagtcgg tgttattcag ctctgggcaa
     1621 gcatcctgtg cggcggtctc tgtggacttg ccggcgctca gctctcgctc tacaacgtga
     1681 ggatgttctc gcgcgacatg acgggcggga ggggcttcgt tgcgctggcc gcgatactca
     1741 tctcgggagg aaaaccgaag agggtcctct tcatctctct tctcttcggc ctctttgacg
     1801 ccttatccgt caagctgcag aacgagaaga tcgcgcccca gttcgccctg atgctcccgt
     1861 acataatgag cgtcgtggtt ctgctgctgt tccatctcaa gacgagaaag caatacggga
     1921 aggcctgagc gatagataaa tcctgcccgg ggcacttttt ctatcacaga gacacaactc
     1981 cctttctgtt gtcgcatacc tgtgtgatat attgaattaa tgccttgaaa gaggttttag
     2041 cagggaggag ataaagtgaa gcgaaattcg gtcaggatat tctttcttct gttggtagtg
     2101 tttctcccac tctcagtggc gatggcgcgg ggagtgacct ttctcgtgaa cccgtcctac
     2161 agccccttca gctacgagga aaatggcgag ctcaagggtc tggcggtaga acttgtaaaa
     2221 ttgctctcgt ctgaattcgg tctcgaagca gagatgacct ctatgccctt cgaagaggct
     2281 tacgcgaagc tgcaggacgg cggtgagtac gctctgccca cgctcgtatt caccccgcag
     2341 cggaaatccc agttcaactg ggtgggccct atcgcgataa cgcccacgta tctttacgcc
     2401 cgcgatacct ctcccgacat tcgctcgata gacgatgcaa agaaagccgg gaaggtcggc
     2461 gccgtgcgcg ggtattactc ccagcaactc ctggccgagc agggtctgga tacggtgatc
     2521 ttcgacgacg aggcggcttt gttgaagagc cttctggagg gtgacatcga tctggcccct
     2581 ttcaacttcg atgtctatac gcgtctgacg gagaaaatac ctgaatcttc aaagttgcgt
     2641 gccacgatcg ttctcgacct cgatatgaca tatataggtt tctcgaaaga cgtaccgacc
     2701 gacacccttc gaaggtggca ggagggactt gacggtctga aaagctccgg cgactttgcc
     2761 gggctttacg aaaaatggct gccgggccgt ccggtccccg gcgtctatac cctgctcacc
     2821 gaggagtacc cgcccgttac atacatgggc gatggaggac ctgcgggctt tgttaccgag
     2881 atagtgagag agctcatgga acgaaacggt atgaaagaag agatcttcct cgttccgtgg
     2941 gcgatcggct atgaactggc ttcgaacctg cccaacgttc ttctcttcag tatggatcag
     3001 acggagcttc gaaagccgct cttccagtgg atcggaccgg tcggcaggaa cacggcttac
     3061 ctttacgggc gagccgattc ggcaatagcg cccgccgatc tcgacgaggc cagaaaggtc
     3121 ggaacgatag ccactacaaa ggactggtgg actgaacagc tcctgagaga tctgggcttc
     3181 acgaatctga agagttttct agaccctctg gacaccgtgc gccagctgat gaacggtgag
     3241 gccgatcttg cgatattcac cgatctcacc gtcagcgaac tggtacagaa cgccggttac
     3301 cagatgaagg atctaaaccg cttgctcgaa attcagacga actatttcta cgtcgcggct
     3361 tccagcggca ccgatcccga aatggttttg attctgcaaa agacgcttga cagcatgaag
     3421 cgcgacggca gcttcgaaga aatccttcgt aaatacgttc ccaatgtgct gatcacaccg
     3481 ttactcttgc agtcgtcgac acccgctacc gtcaggctgc agtcggtagt caactcgctg
     3541 caaattggag agctggccag cttgacactc gaggaaaatg gaagtaccgg ttacatatgg
     3601 gatgtaaggg tctccaaccc gaacgtgctt tcactggctt ttgtaaaaca tggcgtctca
     3661 gatgctggag aagaaagtct cacgggagcc tcttacgaag tagaatggac cttccgggcc
     3721 gaacgtccgg gagagtgcgt gatagttttc tcgctgcgga gaccctggga gagtgtacac
     3781 ccggtgaaga ccttcgcaat aaacgtcaaa gtggaatagt tgaagtggac cataaaaaac
     3841 ggccgggttt cttcccggcc gtttcatttg aaaagtctgt gaggtattac ttcgaatctt
     3901 ctatcatgaa aaagtttaaa gagtatctgc gaaccagtca acagtcgcgg cgatgagttc
     3961 gttgaaagcg ctcatgtcgc cggtgaagat gttgaacgtg tggtccgcgc cttctatcag
     4021 aagcactctg gagtttttgt tgctggacgc atccactatg cgctgcgcgt tgactggatc
     4081 gaccacggta tccattgccc cgttgatagc cagcacggga gcgaaggaat tcgagaagac
     4141 ctccagaaca tcggtgttat atacttcttc gaaccactga agaccgagct tcaacgacgg
     4201 gcgccagtcg aaggtgagtt cgtaatagcc gttggctttg gcgatttcgt agttctcggc
     4261 cgttcctatc gacttgagat cgggagcgcc ggcccagcaa actaccgatc ggaaggagcg
     4321 gttacgaccg gcggccagca tggctatcgt gccaccctgg ctccagccca taacaccgat
     4381 gcggttgggg tcgataccgt tcagtccggc catataatcg aaggctgcgt tgctgtcgga
     4441 tacggcggac atgaagttgt agtttatata gtcggcctcg ctgtcaccgt tgccgatgaa
     4501 atcgaaacga atgctggcga tccccgcttt tgccagggcc ggcgccgtca aaagatagcc
     4561 cccacccgct tcgtctttgt tcgaacccgt gccgtgaagc atcacgactg ccgggaaggg
     4621 accgtctccg tcaggcatgc acacgaccgc cggaatttcg tggtcgccgt ttgtgatcag
     4681 aacggtctgt tccgaaaagc tatggggaat cttcagaatc gtccccacct tcagggagta
     4741 ctcgttctcg ataccgttga aggatgcgat ttccgtccag tccacgccgt acctccgggc
     4801 gatgctccac agggtgtcgc ccttaacgac ggtgtactgc tttgtt
//
LOCUS       DAOOLY010000198         4836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21108_length_4836_cov_5.119640, whole genome shotgun sequence.
ACCESSION   DAOOLY010000198 DAOOLY010000000
VERSION     DAOOLY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21108_length_4836_cov_5.119640"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLD15_09325"
     CDS             complement(<1..774)
                     /locus_tag="PLD15_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPB64151.1"
                     /translation="MRKVWLMIMLLNFLLLSNSLSGGEIRTVFVTAPSSFPDRGKLDA
                     GVAALRNAGFRVVVGRSCQAFLSPREKAQELNEAFSNPEYEAIITARGGYGSYNILDW
                     LDWESIAANPKPFVGYSDITALLLAIYFKTGIVTYHGPMVAVELGSDGQSLKNIMDLF
                     NGNRTIRFNYTSTPVIPGLMTGRLIPANLSLFQALQGTEYLGSLKDAILVLEDVSESK
                     ESLERMIWNIAHINGFSSLRGVIFAGFTEIKNGDLNDIKR"
     gene            complement(865..2898)
                     /locus_tag="PLD15_09330"
     CDS             complement(865..2898)
                     /locus_tag="PLD15_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB64152.1"
                     /translation="MLPALLVLLALISLQFVVRKNWTLGLPAFLIVLLTIVGPILWAL
                     FISFTSYGVYQERPLFAGFDNYLKILEDSGFKLSLKLTALWSFLRTSLEISLSFLLAL
                     KLRKSSRTGRLALLLLALGWFVPSFITVSGWRAFIQGYGGYSLLNIVLGTGVDLTLNP
                     AQAFLATLLVSVWLSIPFSTMIIVGMLGRVSGELDDMMRLDGAGDFAGATLLLGEIRH
                     LMIPYALFQLARATKEFTSVFLLSGNGPLLPEGFTPQTLVGSTSFAGVVLFRKFAAVK
                     DYGLLSAYAVFAGGFAILWILGAVFSRNSVPKRHRQILFLSAVAHLFLGAYTGWSFWF
                     LVPVTAYSLLPFTIPRHRRFFRLTVGALIIYEIIMNVVKISLEGVSGIVPASFLSVPL
                     ILLSMRYRFPSIDFTLSRWLKSSLLILSLLPTVFLVAYILLLSFSVEGEIIPSLNALT
                     AANYPRVIGDGLLNTIFNTLKTALWCFAIILGTIIPLAYLFATKKGFLAKATSSLVLL
                     GSLYTGMHTLLPLYFIFRGIGLTDSFFGVALVVTVQSAPLALLVLGGFFASVPRELRE
                     ISRLDGLSGTGYLWRILFPLSLPVVGGVLTYVLVSCWSSFTLPLIMLNRSEMMPFSLK
                     VFSYAGEIRSYYTSWNLFAAASVIGTVPLLLLFRIARSMIYSPGLGDRGVGYD"
     gene            complement(2900..4006)
                     /locus_tag="PLD15_09335"
     CDS             complement(2900..4006)
                     /locus_tag="PLD15_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64153.1"
                     /translation="MYRFICSLLILTVVVPLAGLTVWVSWEGEDFYREVASSFEREKG
                     IKVELEYFPKIEEKLGVALKTGDLPDMALVKDTYSGNLGASKRALEIAADDLARFKGE
                     YLEAYMYDGTVVALPFYADVQVAFINRTVFDKAGLPLPGELDLGELEAIVQELKKVVQ
                     YPVAFDFTSPYMMFPYISDPSPVGEGGRPFLSGRERVEAVEAVKVYFDTGVLSRLERA
                     AMNGLFRNGKIGIMLQGSYMAEKFAAANLDFAMVPLPDLEGRKIRGVIDSKGFALFKE
                     ESYAQSIEFARYLMEKSEAFCGQYWKYPLFETVQGDPELERIIASGQYMPNGPGYQAM
                     LFEAFEPALQAIFSGAMGVEQALNGAQGYIDSTW"
     gene            complement(4008..>4836)
                     /locus_tag="PLD15_09340"
     CDS             complement(4008..>4836)
                     /locus_tag="PLD15_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPB64154.1"
                     /translation="ATDIGTGSDTILAQIAAEVLSVPLEKIIVYSSDTDVTPFDVGAY
                     ASSTTYVSGNAVRLAAEKVKKQILELAANFLEREEKNLKLSAGIVTCDDGSSVSLEEL
                     CTRHMYTFNQQQIAATASYVGEESPPPFLATFAEVEIDTETGKVYLINLVSYADCGLA
                     LNPIQAMGQLEGASMQGIGWALYEDIIYSTGGRMLTNDFFTYKVPSRMDYGKLTCGLV
                     ESYEPTGPFGAKSIGEIGIDSPIPAIGNAIFDATGIRLRHAPFRSEKIFFEILNLEV"
BASE COUNT         1145 a         1349 c         1341 g         1001 t
ORIGIN      
        1 cctctttata tcgttaagat cgccgttctt tatctcggtg aaaccggcga agattacccc
       61 gcgcagagac gaaaagccgt ttatgtgagc gatgttccat atcattctct cgagcgattc
      121 cttcgactca ctgacgtctt ccagcaccag aatcgcgtct ttcaaggagc ccagatactc
      181 ggtgccctgc agggcctgga agagcgacag gttggccgga atcaatctcc ccgtcataag
      241 gccgggtatc accggtgtgg aagtgtagtt gaaccttatg gtcctgttgc cgttgaagag
      301 gtccattatg ttcttaagcg attggccgtc gcttccaagc tccacggcga ccataggccc
      361 gtgataggtg acgatccccg tcttgaagta gatggccagc aggagtgccg ttatatcgct
      421 gtaacccacg aagggtttgg ggttggcggc gatactctcc cagtccagcc agtcgagaat
      481 gttgtacgat ccgtagccgc ccctggccgt tatgatcgct tcgtactcgg ggttcgaaaa
      541 ggcttcgttc aactcctggg ccttctcccg cggagagaga aaggcctggc agctccttcc
      601 gacgacgacc ctgaaaccgg cgttcctcag cgcggcgaca ccggcgtcga gtttgcccct
      661 gtcgggaaaa ctagaaggag ccgtcacgaa gactgtcctt atttctcctc ctgagagcga
      721 gttgctgaga agcaagaagt tgagcagcat aatcatcaac cagacctttc tcattagaat
      781 atcctccagt gaaagctata cttaatagtt tgccatcgac gggagtcaaa atcaataagc
      841 ctttgaaaag attcttcgca aaactcaatc ataacctacg cctctatcgc ccagaccggg
      901 gctgtaaatc atcgaccgag cgatcctgaa aagcaggagg agcggtaccg tgccgataac
      961 cgaggccgcg gcgaagaggt tccaggaagt gtagtaactc cttatctcgc cggcgtagga
     1021 gaaaactttc agcgaaaagg gcatcatttc gctccggttc agcatgatca ggggcagcgt
     1081 gaaggaactc cagcaggaaa ccagtacata ggtcagaacc ccgccaacga ccggcagcga
     1141 aagggggaag agtatcctcc agaggtagcc ggtcccggag agaccgtcga gcctagatat
     1201 ctccctcaat tcacgaggca ccgaggcgaa aaagccgccg agaacgagca gggccagagg
     1261 cgccgattga accgttacga ccagggcgac cccgaagaaa ctgtcggtca gaccgatacc
     1321 cctgaatatg aagtagagag gcaacagtgt gtgcatcccc gtgtagaggc ttcccagcag
     1381 caccagcgag gacgtcgcct tcgcaaggaa gccttttttg gtggcgaaga gataggccag
     1441 aggtattatg gttcccagaa ttatcgcaaa gcaccagagg gccgttttaa gagtgttgaa
     1501 gatggtgttc aaaagcccgt cgcctatgac cctcggataa ttcgccgctg tgagcgcatt
     1561 cagcgaaggg attatctctc cctctacgga gaaggagaga aggagaatgt acgcgacgag
     1621 aaaaaccgtg ggcaggagcg agaggatcag cagcgaactt ttgagccatc gggaaagcgt
     1681 gaagtcgatc gagggaaacc tgtacctcat cgaaaggaga atcagaggaa cgctgagaaa
     1741 gctggccggc actatcccgc tcacgccctc cagcgagatc ttcaccacat tcatgattat
     1801 ttcgtatatg atcaaagctc cgacggtaag gcggaaaaac ctcctgtggc gcggtatggt
     1861 gaaggggaga agcgagtagg ccgttaccgg taccagaaac cagaagctcc agccggtgta
     1921 ggcccccaga aagagatgcg ccacggcgct caggaagagg atctgtctgt ggcgtttcgg
     1981 gacgctgttt ctggagaaga cggctccgag tatccataga atcgcgaagc cccccgcgaa
     2041 gacggcgtag gccgagagga gaccgtaatc cttgacggcg gcgaacttcc tgaagagaac
     2101 taccccggca aaagaggtcg agccgaccaa cgtctgtggt gtgaaaccct ccggcagcaa
     2161 cggcccgtta cccgaaagga gaaagaccga tgtgaactcc ttcgtcgccc ttgccagctg
     2221 gaagagggcg taaggtatca tgagatggcg tatctccccc agaagaagcg tcgcgcccgc
     2281 gaaatcacca gcgccgtcaa gcctcatcat atcgtccagc tccccggaaa ccctgccgag
     2341 cattccgact attatcatcg ttgaaaatgg tatcgacagc cagacgctca ccagaagcgt
     2401 ggcgaggaag gcctgggccg gattgagcgt gagatcgacg cccgttccca gaacgatgtt
     2461 cagcaaagag tagccgccgt agccctgtat gaaggccctc catcccgaca cggttatgaa
     2521 ggagggaacg aaccagccga gcgccagcag tagcagcgcc agccttccgg ttcttgagct
     2581 ttttctcagc ttcagggcta gaagaaacga gagcgatatc tccagcgagg ttctaagaaa
     2641 ggaccacaaa gccgtcagct tgagcgagag tttgaagccg gaatcttcga ggattttcag
     2701 atagttgtca aaaccggcga agagaggcct ctcctgatag acgccgtaac tggtgaagga
     2761 gatgaaaagg gcccagagaa tagggcccac gattgtcagc agaacgatca ggaaggctgg
     2821 aagaccgagc gtccagttct tcctgaccac gaactgcagc gaaattagag cgagtagaac
     2881 gagcagcgct ggaagcatct caccaggtcg aatctatgta tccctgtgct ccgttcaacg
     2941 cctgctcaac acccatcgca ccactgaaaa tggcctgaag ggccggctcg aaggcttcaa
     3001 agagcatcgc ctgatagccc ggcccgttgg gcatgtactg tccgctcgcg atgatcctct
     3061 ccagttcggg atcgccctgg acggtttcga agagcgggta tttccagtac tgcccgcaga
     3121 aggcctcgct cttctccata aggtatctcg cgaactctat agactgcgcg tagctttcct
     3181 ctttgaatag ggcgaagccc ttggaatcta tgacacccct tatcttcctg ccttctagat
     3241 ccggtagcgg caccatcgca aagtccagat tcgcggctgc gaacttctcg gccatatagc
     3301 taccctgaag cataatccct atcttcccgt ttctgaagag gccgttcatt gcagccctct
     3361 ccagtctgct tagcactccg gtgtcgaagt ataccttgac cgcttcgacg gcctctacac
     3421 gttccctgcc gctcaagaac ggccttccgc cctctccgac cggagatggg tcggaaatgt
     3481 agggaaacat catgtagggg gaggtgaaat cgaaggcgac ggggtactga acgactttct
     3541 tcagctcctg cacgatcgct tcgagctctc ccagatcgag ctcaccggga agtggcagcc
     3601 cggccttatc gaagacagtt ctgttgatga aggctacctg gacatctgcg taaaagggaa
     3661 gcgccacaac ggtcccgtcg tacatatagg cctccaggta ctcccctttg aaacgggcca
     3721 gatcatcggc ggcgatttcg agcgctctct tcgaagctcc cagattcccc gagtaagtat
     3781 ccttgacgag agccatgtcg ggcaggtcgc ctgtctttag agcgactcca agcttttcct
     3841 cgatcttggg gaaatactcc agttcgacct ttattccttt ttcccgctcg aacgacgagg
     3901 cgacttccct gtaaaagtcc tcgccctccc agctcaccca gacggtcagc cctgcgagcg
     3961 gaacgacgac tgtcagtatc aaaagactgc aaataaacct gtacatttca aacctccaga
     4021 ttcagtattt cgaagaagat cttctcgctc ctgaaggggg catgtctcaa tcttattcca
     4081 gtggcatcga agatcgcgtt cccgatcgcc ggtatcggcg aatctatgcc tatttccccg
     4141 atgcttttcg ctccgaacgg cccggtgggt tcgtagcttt cgaccagtcc gcaggtcagc
     4201 ttcccgtaat ccatcctgga gggaaccttg taggtgaaga aatcgttggt gagcatccgg
     4261 ccgccggtgg agtagattat atcctcgtac aacgcccagc ctatgccctg catcgaggca
     4321 ccctccagct gtcccatggc ctgaatgggg ttgagtgcca gcccacagtc ggcgtaagag
     4381 acgaggttta tcaggtaaac cttgccggtt tcagtatcga tctcgacttc ggcgaaggtg
     4441 gcgaggaagg gcggtggcga ctcctcgccc acgtaggagg ccgtagcggc gatctgttgc
     4501 tggttgaagg tgtacatgtg cctggtgcag agctcctcca ggctcaccga gctcccatcg
     4561 tcgcacgtca cgattccggc cgaaagcttc agatttttct cttctctctc caggaagtta
     4621 gccgccagct cgagtatctg ctttttgacc ttttcggcag ccagcctcac cgcgttgccc
     4681 gagacgtagg tagtactcga ggcgtaggcg cctacgtcga agggtgtaac atcggtgtcg
     4741 gaagaataga cgattatctt ctcgagggga acggagagga cctcggccgc gatctgggcg
     4801 aggatggtgt cgctcccggt gccgatatcg gtggcg
//
LOCUS       DAOOLY010000199         4824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21176_length_4824_cov_4.510380, whole genome shotgun sequence.
ACCESSION   DAOOLY010000199 DAOOLY010000000
VERSION     DAOOLY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21176_length_4824_cov_4.510380"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..183
                     /locus_tag="PLD15_09345"
     CDS             58..183
                     /locus_tag="PLD15_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB64155.1"
                     /translation="MELSRAYYNDIVTFYNTRLERIPDVLVAKIARLTPQGLFKN"
     gene            complement(241..1932)
                     /locus_tag="PLD15_09350"
     CDS             complement(241..1932)
                     /locus_tag="PLD15_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPB64156.1"
                     /translation="MAGKKLLIIAIMVTFLSVMALSISFEISIDEATVGEIAALGLET
                     PINGRVFVIVTKDSSSEPMYQTDVRGVPFWGKDVFGFTSDDLVSISSDDLEVIGYPIK
                     IEELPAGDYYVQAFLNVYTTFNRADGHTVLMHDDTGDGQWFWESIGNAYSVAKKTYID
                     PMNWGTIKLTLSKVFKPDYELEPGMVLQQGNYKDTEWVKYVKIKSEAVSEFWGKDMYI
                     GANVLLPKGYYDNPDVYYPVIYYQGHFPGSSAPVGFRVNNNVYKFWTSEGIANFIAVS
                     FRDATPYYDTSYSVDSVNSGPYGTAITEELIPYLESQFRMIPEKWARILAGGSTGGWE
                     TLAMKVFYPDIFGRTYVWYPDGVDFNYYQLINIYNDDNAYYSDFGWLKTERPSARDTH
                     GNIRFTVKQENYFEMAAGPSNRSGGQWSVWESTYGPVGVDGFPQPIWDQVTGEINKNV
                     AEYWKENFDLNYILQENWEELGPKIAGDVHVAVGDMDNYYLNEAVYLLKNAMEKMENP
                     VSDMTFDFGPNQGHGWKGWSPTKPNVALANEEWLEQLSNYMIEHAPENANKNWIY"
     gene            complement(2360..2599)
                     /locus_tag="PLD15_09355"
     CDS             complement(2360..2599)
                     /locus_tag="PLD15_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HPB64157.1"
                     /translation="MFYRSRDRGIFNLELFVNHGARGKSYIATLSQKEEMVIENLRGE
                     EDALLFEEFIWASLQRVRDYIEFKDFESFSHGRRQ"
     gene            complement(2641..3654)
                     /locus_tag="PLD15_09360"
     CDS             complement(2641..3654)
                     /locus_tag="PLD15_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64158.1"
                     /translation="MLEVLSIKNLTKLFPLPGRFFRTSRSTLRNVHAVDDVTFEISDN
                     DSLALVGESGCGKTTTGRLIVGLEKPSGGRIFFEGKEVTDRLTDGKHANNVDRSDIGE
                     IRRKIQMIFQDPYDSLNPRMTIDEIVSEPLVVNQLGTAKDRTVKVKETLEAVGLSPAS
                     AYTQRYPHELSGGQRQRIAIARAVILNPAFIVADEPTSMLDVSVRAGIMELFRNLTER
                     IGATYLYITHDLAVARYMVQRIAVMYLGKIVEIGPTEKILANPLHPYTKALLQAVPVP
                     DPEVQKGEPDILGQISKPIDPPDYCRFYDRCPLRIDSCAREKHPPLRDYDNGHCAACY
                     VID"
     gene            complement(3647..4639)
                     /locus_tag="PLD15_09365"
     CDS             complement(3647..4639)
                     /locus_tag="PLD15_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64159.1"
                     /translation="MNHPVLEVRDLKMHYTIKGGSVKAVDGVTFSLAKGEALGVVGES
                     GCGKTSLAMAILRLLTENASFVSGSIFFSKDGDKIDLVRLGEEEMRRYRWKEISMIFQ
                     AAMNSLNPVYRVGDQIAEAMRTHFPGISKEEISDRISYLFRLVGLDPARVNRYPHEYS
                     GGMKQRAIIAMALICNPSVVIADEPTTALDVIVQGRILKRLRNIQEESKTSIIYISHD
                     IAAIAQVCDRIAIMYAGKIVELAGTAAILKRPAHHYTSALMAAFPSITGQKKKLVAIE
                     GEPPDLVNPPVGCRFASRCGAAEKICFESEPECAEIGEEHLAACHFPLEVEIHA"
BASE COUNT         1152 a         1204 c         1095 g         1373 t
ORIGIN      
        1 ttcaatccct ttttcttctc ttcatctctt tcctgttaac ttggggcagg gtcagggata
       61 gagctttcaa gagcgtacta caacgatata gttactttct acaacaccag gctcgaaagg
      121 attccagatg tattagtggc aaagatcgcc aggcttactc cgcagggact tttcaaaaac
      181 taattaaaaa gaccggaagg gctctccctt ccggtcattc cagcattctg aatcggttta
      241 tcagtagatc cagttcttgt tcgcgttctc cggagcgtgc tcgatcatgt aattagatag
      301 ctgttccagc cattcttcgt ttgccagcgc aacgttcggt ttggtcgggc tccagccttt
      361 ccagccgtgg ccctgattgg gaccgaaatc gaatgtcata tctgacacgg gattctccat
      421 cttttccatt gcattcttga gaagatatac cgcttcgttc agatagtagt tgtccatatc
      481 gccgaccgcc acgtgaacgt ctccggcaat ctttggtccc agttcttccc agttctcttg
      541 aagtatgtag ttcagatcga agttttcctt ccagtattcg gcaacattct tgttgatctc
      601 gccggttacc tgatcccaga tcggttgagg gaagccatcg actcccaccg gaccgtaggt
      661 cgattcccac acagaccatt gaccgccgga cctgttgctc ggaccggcag ccatttcgaa
      721 gtagttttcc tgcttgacgg tgaatcggat attaccgtgt gtgtctctgg cactgggtct
      781 ctcggttttt aaccatccaa agtcactgta ataagcgtta tcgtcgttgt agatgtttat
      841 cagctggtag tagttgaagt caacgccatc gggataccag acataggttc taccgaagat
      901 atcggggtag aagactttca ttgcaagagt ttcccagcct ccggtagatc cgccggccag
      961 tattctggcc cacttttccg gtatcattcg gaactggctt tcgaggtatg gaatcagttc
     1021 ttcagttata gccgtcccat aaggcccgga gttcacagag tccacagaat aagaggtatc
     1081 gtaataaggc gttgcatccc tgaaggaaac ggctatgaag ttggctatcc cttcggaagt
     1141 ccagaacttg taaacattgt tgttgaccct gaatccgact ggagcgctcg acccggggaa
     1201 gtgtccctgg taatagataa ccggatagta cacatcgggg ttgtcgtagt aaccttttgg
     1261 aagcagtaca ttggcgccga tgtacatatc ctttccccag aactccgaaa ccgcttcgct
     1321 cttgatcttt acgtatttga cccattccgt atctttgtaa ttaccctgct gaagtaccat
     1381 tcccggttcc agttcataat ctggtttgaa tacctttgag agtgtaagct ttatcgttcc
     1441 ccagttcatg ggatcgatgt aggttttctt cgcgacgcta taggcgtttc caatggattc
     1501 ccagaaccac tgtccgtcac ctgtatcgtc gtgcatcagc accgtatgac cgtcggccct
     1561 gttgaaggtt gtatatacgt tcaaaaaggc ctgaacgtag taatcgccgg ccggtagttc
     1621 ttcgatcttt attggatagc cgatcacttc aaggtcgtcg gaagatatac taaccaggtc
     1681 gtcactggtg aatccgaaga catccttccc ccagaagggg acacctctga catccgtctg
     1741 gtacatcggc tcgctagatg agtctttggt gacaataacg aaaactctgc cgttgatagg
     1801 tgtttcgagt ccaagagcgg cgatttcacc aactgtagct tcatcgattg atatttcaaa
     1861 gctgatggat agagccatca cgctcaagaa agtaaccata attgcaatga taaggagctt
     1921 tttccctgcc attttccacc tccatgtagc tgatatacaa ctggctcata ccttcgaccg
     1981 aagaattctc cgatcggaag agcagttcga aaattacgga atttgagaat gatgaattaa
     2041 aaaaactaaa atgaatcaga atgctggaag tactatagat agattatcgg atagtgtcaa
     2101 cgaagacgaa gagaggattc tctgcgcgaa acgaagaagt gtgcgccgct agcagtatta
     2161 gcaagagctt ttggtgaaag gaaagccttt aagaaatctg acaattccat ctgccttacc
     2221 ccctcttcac cggtcgatac gattcaccgg ttgttattgg cattatacat cgcatccttt
     2281 ggtttttgaa cgccgtgcgg acttatttac atttttgttc gtattccgta tagttacgga
     2341 cggtcgccga ccatgatttt tattgtcttc gtccgtgaga gaagctctca aaatccttga
     2401 attctatata gtcgcgcacc cgctggagtg aagcccatat gaactcttcg aagagaagag
     2461 catcttcctc gccccggaga ttttcgatta ccatttcctc tttctggctc agggtagcta
     2521 tatagctttt gcctctggcg ccgtgattca caaagagttc aaggttgaaa attcccctat
     2581 ctctagatct gtagaacaaa gaaaccacct ccatagtttt ttactggtgt ttggcagtat
     2641 tcagtcgatc acatagcaag cagcacagtg gccattatcg taatccctga gcgggggatg
     2701 tttttctctg gcacagctat ctattctcag ggggcagcga tcgtaaaacc tgcaataatc
     2761 tggaggatct atcggcttgg atatttgccc cagtatatct ggctcaccct tttgaacttc
     2821 cgggtccgga acgggtacgg cctgcagtag tgctttagtg tatgggtgaa gcgggtttgc
     2881 aagtatcttc tcggtcggtc cgatctcaac gatctttcca agatacatca ccgctattct
     2941 ctgaaccatg tacctggcga cggccagatc gtgcgtaata taaagatagg tagctcctat
     3001 tctttcggtg agatttctga aaagctccat aattcccgct cttaccgaaa catcaagcat
     3061 ggatgtaggc tcatcggcca ctatgaaggc cgggtttagt ataacggcac gcgctatcgc
     3121 tattctctgg cgctggcctc ccgaaagctc gtgagggtac cgttgcgtgt acgctgaggc
     3181 cggagagagt ccgacagctt ccagtgtctc tttcactttc actgtcctgt ctttcgccgt
     3241 gcctaactgg ttaaccacta gaggttccga tacgatctcg tcgatcgtca ttcttgggtt
     3301 gagcgaatcg tatggatcct ggaaaatcat ctgtatcttt cgtcgtattt ctccgatatc
     3361 agatctatct acattgttag catgtttacc gtcggtgagc cggtcggtta cctcctttcc
     3421 ctcgaagaat attctccccc cggaaggttt ctcaagacca actattagtc tgcctgtggt
     3481 tgtcttaccg cagccggact ctcccactag agccagggag tcgttgtcgg agatttcgaa
     3541 ggtgacatcg tccacggcgt gaacatttct caaggtagat cttgaggttc tgaaaaacct
     3601 tccaggcagt ggaaaaagct ttgtgagatt ctttatagag agaacttcaa gcatggattt
     3661 ccacctccag cggaaaatgg caggctgcca gatgttcttc accaatctca gcacattcgg
     3721 gttcactttc aaagcatatt ttttcggccg ccccgcatct ggaagcgaaa cggcaaccta
     3781 ccggaggatt gaccagatca ggtggctctc cctctatagc tacaagtttt ttcttttgtc
     3841 cggttatcga cggaaaggct gccatcaaag ctgaagtata atggtgagcc ggtctcttga
     3901 gtattgctgc ggttcctgcc agttccacta tcttaccggc gtacatgatc gctatcctgt
     3961 cacagacctg ggcaattgcc gctatatcat gagagatgta gataatcgat gttttcgact
     4021 cttcctgaat attcctcagc ctcttcaata tcctgccctg aactatcacg tctagggccg
     4081 tcgtcggttc atctgcgata acgaccgaag gattgcagat cagtgccata gctattatag
     4141 cccgttgttt cattcctccg ctgtattcgt gtggatagcg attcactcta gcagggtcaa
     4201 ggcccacaag gcggaagagg tacgaaatcc tgtcggatat ttcttctttc gaaatgcctg
     4261 gaaaatgtgt cctcatggcc tcggctatct gatctccaac cctgtaaacg ggattcaggg
     4321 agttcatggc ggcctgaaaa atcattgaga tctctttcca cctgtaacga cgcatttcct
     4381 cttctcccaa cctcaccaga tctattttat cgccgtcctt cgaaaagaaa atgcttccac
     4441 tgacgaagct ggcattttcc gtgagcaacc tgagaatcgc catcgcaagc gaggtctttc
     4501 cgcatcccga ttcgcctaca acaccgagcg cttctccttt tgcaagactg aaagtcactc
     4561 catccacggc cttcaccgag ccgcctttaa ttgtgtaatg cattttgagg tctctcactt
     4621 ccagtaccgg atggttcaag tcggaccccc ctcaaagagt tcatagccaa caggccaatt
     4681 atatcattca agcattgcag cccttcgtta ctgagaacga tggagagtgg agcgaaattt
     4741 aattgcaatt ttgttactat ctgtcttttc aggtattata tatgaccgta tccaataaat
     4801 aggcgaggaa agaaataagg caaa
//
LOCUS       DAOOLY010000200         4775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21494_length_4775_cov_5.679873, whole genome shotgun sequence.
ACCESSION   DAOOLY010000200 DAOOLY010000000
VERSION     DAOOLY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21494_length_4775_cov_5.679873"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..2246
                     /locus_tag="PLD15_09370"
     CDS             45..2246
                     /locus_tag="PLD15_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPB64160.1"
                     /translation="MRVAVEKNGAVRYPTGAVITSPEEELESGGANGFIPGIDFHATG
                     RPGSISIEESAEGFVIRITLGEDQGVFGLGQEIGPLNKRGRIYEMYNTDDPDHSPSKT
                     RLYSTLPVFYLISPGGCTGFFLDHSGYSKFDVAFEKRDTLLISVEGSAFDLYVMSGTP
                     GEMIKKIFRLTGKPLFLPVWSLGFQQSRWSYPDEESVMNIASKMREKEIPCDVIYLDI
                     DYMDDYKVFTWNARRFPDPRSMVKRLEEMGFKVVTIVDPGVKAAEGYGVFEEGKRENV
                     FCKREDGRDFRPAVWPGESRFPDFLNSKARKWWGDLYREFVELGISGFWNDMNEPSIF
                     YTAESLADLSEMMKALKNDGGIETDALFGKVVFLKKHYDHGRDFYQEDDAGIRHLHRN
                     VRNVYGFNMARAVFEGLKRVRPEERVFSITRSSYTGIQRYAILWTGDNESQWEQLSSE
                     IKMVQSISLAGVSFTGCDVGGFGGNCHGELLARWTQFGAFLPFFRNHSAMGTRPQEPW
                     AFDDEIERIVKKTVELRYSLLPYIYSVLRDSSEGNSTMIRPLVMIWPEDRDTYQADDQ
                     YMFGPSLMVAPVYTLNARGRHVYLPGCDWLNLSSGEIVRKGHRWAEAPLDTVPLYLKE
                     DSLIFSTEPSQYLESAVWSRIEVKGFVRNRAVFDLYEDDGTSEAYTRGEYSLKRITVE
                     KLNGGILVDISPQEGELKLPEREVSIEITDGDRTYRTTLADSSEGARISIK"
     gene            complement(2235..4706)
                     /locus_tag="PLD15_09375"
     CDS             complement(2235..4706)
                     /locus_tag="PLD15_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64161.1"
                     /translation="MSGKFFLSVFLLFTVISFGYTFRDFDWYQHESEHFIYIYHPEIE
                     ESVPLVEEIAEESYTLLSRFFGNHLSRKIAIVLWGYEDNPNGLANPLIDSVYSITIGL
                     NYVHREDGYWLRTVIGHELSHIFQLTSNGPFGSLIRKYLSGAYLPGALQPMWLSEGFA
                     QFGSHLLKADGYDYRRVSFLKDQLALETPFSEETIVAGYGPIGGEAYYNFGFAFLMYL
                     ADQYGDEKLREFSSLKSGILGLAGIEPAFSIVFGRSYRQLKEDFIARGRETFDLDEPV
                     SLYNSPTENSQTLQEYSPKSCGGKLYYAIYDRDRGYFSLNGNGVELLSTTMEILDYSV
                     FENEIAIVLLEKQNGSEARLYIFKDGKLTRTKHSGILSLEYLGKERLAVLKNEKGVLR
                     VQTLSLRNDRTSDIFVAPDSRVQISTVRATGDGTQIAFRVNFEGRKYLALYLFRENEL
                     GFYEFESDFEMGSCFSDGFLVSVQRELSSDVFLFRSGGTMEGLASFSRYVRDPVRVKN
                     TVIAAGQLNGLKLLKSDAPVPAARSVPRGTLEIEEIRPIESRKRYDGLNSWRFVSVIP
                     FDGVSLLFSDPTFNNHLIAGGSLNFETLEPKLHLQLSSSNYLAVDFEGKAKLTGLKPE
                     AYLDIYRQYPLTPKLSFGWKVGLEYPLKLYGSLNVMLEDASALYGGQARLKTSFTVRD
                     TQTATANVADASINVNLEWTRSNFELSSRIFSRVTLGKNSSVIPVKLWNFENDSSIVV
                     GLSVSSEYILSRRNLNFFNLVHLSKEGIGGRIDILLGSKLSWRLVLYKIETVFLYAAY
                     PFEVKAGLMLEGARLLPYFDFELLY"
BASE COUNT         1147 a         1241 c         1323 g         1064 t
ORIGIN      
        1 gatgaaaaat ggggagaagc ttttaccgct acgcggattc agttttgaga gtcgccgttg
       61 aaaagaatgg agcggttaga tacccgacgg gcgcggtgat aacctctcct gaggaagagc
      121 tggagagcgg cggtgcaaac ggctttatac ccgggataga tttccatgcg accggccgac
      181 ccggctcgat ctccatcgag gagtccgcgg agggtttcgt gatcaggata acgctcgggg
      241 aagatcaggg agtcttcggg ctcggtcagg agatcggtcc cctcaacaaa agggggagga
      301 tatacgagat gtacaacacc gacgatcccg accattcccc ctccaaaacg cggctttact
      361 ccaccctgcc ggtcttctat ctcatatctc cgggcggatg cacgggcttc ttcctcgatc
      421 actccggtta ctcgaagttc gacgttgcct tcgaaaagag agatacgctg ctcataagcg
      481 tagaaggtag cgccttcgat ctttacgtta tgtccggaac gcccggggag atgataaaga
      541 aaatcttcag gctgaccgga aagccgctgt tcctgcccgt atggtcgctc ggcttccagc
      601 aatcgagatg gtcctacccc gacgaggaga gcgtgatgaa catcgcctcg aagatgcgag
      661 aaaaggaaat cccctgtgac gtgatctatc tcgatataga ctacatggac gattacaagg
      721 tcttcacctg gaacgccagg cgtttccccg atccccggtc catggtgaag aggctcgaag
      781 agatgggctt caaggtcgta acgatagtcg atcccggtgt caaggccgcc gaaggatacg
      841 gggtcttcga agaggggaag agagaaaacg tgttctgcaa aagggaagac ggccgggact
      901 tcagaccggc ggtgtggcct ggcgagtcga ggtttccgga tttcctgaat tcgaaggcga
      961 gaaaatggtg gggagacctg tacagggagt tcgttgagct gggcatctcc ggtttctgga
     1021 acgatatgaa cgagccctcg atcttttaca cggccgaatc tctcgccgat ctctccgaga
     1081 tgatgaaggc tctcaaaaac gacggcggta tcgagactga tgctcttttc ggaaaggtgg
     1141 tctttttgaa aaagcactac gatcacggcc gggactttta ccaggaggac gatgccggca
     1201 tcagacacct ccacagaaac gtcagaaacg tgtacggttt caacatggca agggcggtct
     1261 tcgaaggcct gaagagggtc agaccggaag agagggtctt cagcataaca cgctcctctt
     1321 acacggggat tcagcgttac gcgatccttt ggaccgggga caacgagagt cagtgggagc
     1381 agctttcgag cgaaataaag atggttcagt cgatctctct ggccggggtt agcttcaccg
     1441 gctgcgatgt cggaggcttc gggggaaatt gccacggcga actgctggcc agatggacgc
     1501 agttcggcgc cttcctgcct ttcttcagaa accacagcgc catgggcacc agaccgcagg
     1561 agccctgggc cttcgacgac gaaatcgaaa ggatagtcaa aaagaccgtg gagctcagat
     1621 actcgctcct cccctacatc tactccgttc tcagggactc gagcgaggga aattccacga
     1681 tgatcaggcc cctggtcatg atctggccgg aagacagaga cacataccag gccgacgatc
     1741 agtacatgtt cggcccttcg ctcatggtag cccccgtata taccctcaac gcaaggggac
     1801 gccacgttta cctccccggc tgcgactggc tgaacttgag cagcggagag atcgtccgga
     1861 aaggtcatcg ctgggccgag gctccgctgg acacggtacc gctctatttg aaggaagact
     1921 ccctcatatt ttccacagaa ccttctcagt atctggaaag cgctgtgtgg agccgaatcg
     1981 aggtaaaagg tttcgtcagg aaccgggccg tcttcgatct gtacgaggac gacggtacga
     2041 gcgaggcata cacccgtggc gagtattctc tgaagaggat cacggtggaa aaactgaacg
     2101 gcgggatcct cgtagatatc tctccgcagg agggtgagct gaaactcccg gagagggaag
     2161 tatctatcga gataaccgat ggagacagga cttacagaac aacgctggcc gactctagcg
     2221 agggcgcgag gatatcaata aagtaattcg aagtcgaagt aaggcagcaa tctggcgcct
     2281 tccagcatca ggccggcctt cacctcgaat gggtatgccg cgtagagaaa aacggtctct
     2341 attttgtaaa ggacgaggcg ccacgatagt ttcgaaccca ggagtatatc tatcctgcca
     2401 ccgatcccct ctttcgagag gtgtacgagg ttgaagaagt tgaggtttct cctggatagt
     2461 atgtattctg agctcacgga gaggccgacg acgatgctgg aatcgttctc gaagttccag
     2521 agcttcaccg gaatcacgga agagtttttg cccagcgtga ctctcgagaa tatccgcgag
     2581 ctcaattcga aattgcttct cgtccattcc aggtttacgt ttatgcttgc atcggctacg
     2641 ttcgccgttg ccgtctgggt gtccctcacg gtgaaggagg tttttaaacg ggcctgacct
     2701 ccgtaaaggg ccgaggcatc ctcgagcata acgttcaaag aaccgtagag cttcaacgga
     2761 tattccagtc cgaccttcca gccgaaagag agcttcgggg tgagggggta ctgcctgtat
     2821 atatccagat atgcctcggg tttcaaccct gtcagcttcg cctttccctc gaaatcgacg
     2881 gccagatagt tgctgctaga cagctgaaga tgcagtttcg gttccagtgt ctcgaaattc
     2941 aaggaaccgc cggcgatcaa atggttgttg aaagtcggat cggagaagag cagggacaca
     3001 ccgtcaaagg gaattaccga gacgaaacgc cagctgttga gaccgtcgta ccgtttcctt
     3061 gactcgatgg gccgtatttc ttctatttcc agagtgcccc gcggaaccga tctcgccgcc
     3121 ggtacaggcg catcgctttt caagagcttc aagccgttaa gctgtccggc cgcgatcacc
     3181 gtgtttttca ctctcacagg atctctgacg tacctggaaa aactggccag gccttccatg
     3241 gttccgccgg atctgaagag gaaaacgtcg ctcgagagct ctctctgaac ggagaccaga
     3301 aagccgtcgg agaaacagct gcccatctcg aaatcactct cgaattcgta aaaacccagt
     3361 tcattctctc tgaacaggta gagagccagg tattttctcc cctcgaagtt taccctgaag
     3421 gctatctgtg ttccgtcgcc ggttgccctc accgtactta tctgaacgcg gctatccggc
     3481 gcaacgaaga tatccgaggt tctgtcgttt ctcagcgaca gtgtctgtac cctcagaact
     3541 cccttctcgt ttttcaatac ggccagacgt tctttgccga ggtattcgag actgagtatg
     3601 cccgaatgtt tcgtcctggt cagcttgccg tctttgaaaa tgtacagtct ggcctccgat
     3661 ccattttgct tttcgagcag gacgatggcg atttcatttt cgaagacgga gtagtccagt
     3721 atctccatgg tggtgctcag cagttcaaca ccgttgccgt tgagggagaa gtagcccctg
     3781 tctctgtcgt agatcgcgta atacagtttc ccgccgcagg acttgggcga gtactcctga
     3841 agcgtctgcg aattctccgt agggctgttg taaagagata caggctcgtc cagatcgaac
     3901 gtctcccttc cccgggctat gaaatcttct ttcagctgcc ggtaactcct gccgaaaacg
     3961 atagaaaaag ccggttcgat tccggccaga ccgaggatac ccgatttcag actggaaaac
     4021 tcccggagct tttcatcgcc gtattggtcg gcaagataca taaggaaggc gaacccgaaa
     4081 ttgtagtagg cctcgccgcc gatcgggccg tatccggcga ctatggtctc ctcggaaaag
     4141 ggagtctcca gcgctagctg atccttgagg aaagagactc tccggtagtc gtaaccgtcg
     4201 gccttcaaga gatggctgcc gaactgggcg aagccctcgg acagccacat cggctggagc
     4261 gcaccgggga gataggctcc ggataagtat tttctgatca gagagccgaa aggtccgttc
     4321 gaagtcaact ggaagatatg gctcaactcg tggccgataa ccgttcttag ccagtagccg
     4381 tcctctctgt gaacatagtt caggccgatc gttatggaat agaccgaatc gatgagggga
     4441 ttggccagac cgttgggatt gtcttcgtac ccccacagaa cgatggcgat cttccttgag
     4501 agatggtttc cgaagaaccg gctgagcaga gtgtaactct cctcggctat ctcctcgacc
     4561 agcggaacgc tctcttctat ttcggggtgg tagatgtaaa tgaaatgctc tgactcgtgt
     4621 tggtaccagt caaaatccct gaaggtatat ccgaaagaaa tcacggtaaa aagcaagaaa
     4681 acggacagga aaaactttcc ggacaattgt aaagcaccac cctctggaat atatgaatct
     4741 gtactactat tatagactat gatgtctatc tgaag
//
LOCUS       DAOOLY010000201         4775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21495_length_4775_cov_4.897034, whole genome shotgun sequence.
ACCESSION   DAOOLY010000201 DAOOLY010000000
VERSION     DAOOLY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21495_length_4775_cov_4.897034"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1249
                     /locus_tag="PLD15_09380"
     CDS             92..1249
                     /locus_tag="PLD15_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB64162.1"
                     /translation="MNIAMFSDTYAPQVNGVVTMIKMLEENLQKRGHNVYIFTVDHPK
                     ATSQENVYRVPSLRFPWEKQHRIGLPTNYRELIQIITNLKIDLIHSHTSIIVGYLANL
                     VTSKLGLPGVTTYHTMMEEYVHYIPFMEPILRVYIRKQDKRFCDRNRAVIAPSIKIKK
                     LLETYGVSSHIEVIPNGVDLTPFRREISEEERRNFRNRYNIGEEDPVMIFVGRMGGEK
                     SIDKLIENFARVNGEIPNSHLLLVGDGPLRTQLKELSVELGVGSNLHFTGFLKWPAEI
                     SLAYKSSDLFIIASHTETFGLVTLEAMASGLPVAAYKDDSIANMVFDRDNGFMCPSKD
                     DLYKVIIEVLKDDSMRKRMSERSREISLDFSAEANTEKTLDLYGRVLRNEV"
     gene            complement(1233..2243)
                     /locus_tag="PLD15_09385"
     CDS             complement(1233..2243)
                     /locus_tag="PLD15_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB64163.1"
                     /translation="MKRSKRNFLIALSLSVVIIIVILGATDFRKSISYLLNAKPLWIA
                     LIFGLSICSWFFEALTLKVFAVMRSLSVPLTYLFKITVIGTFFNGITPFSSGGQPAQI
                     VIMQRRNISVGESTAMLVSRFLIYQSVITVLGVMAIFGAYPVVSGKISNLAVLSIFGF
                     LINSAVLFFLVLFSLSPGFTAKTISVIVKILGWLRIVKNVDLAIEKSLKELGFFHSSM
                     SELMKRPLVLILASLATFAQLICQISIPYFVALSMGIEMNYFEITAVQLILFLVVSLI
                     PTPGASGVSEGGYLLFFRPFFGESIGAALLVWRFFSYYVNIIFGGLATAHEIGFKSKL
                     RS"
     gene            complement(2240..3244)
                     /locus_tag="PLD15_09390"
     CDS             complement(2240..3244)
                     /locus_tag="PLD15_09390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB64164.1"
                     /translation="MKVLLYSEGKKLFSKSGVGRALKHQMIALEKVGVAYTTDEEDNY
                     DLAHINTIGPGGEKVLKKSRKSGLPVIYHTHTTFEDFRNSFMFSNSLAPLIKSRIKRL
                     YSSADFLISPSEYTRDLVRGYGVNLPIKVISNGVDKEKFSRNAFLAESFRKQYGIEGP
                     LVISVGLPFSRKGVVDFCDIAANKADIRFFWFGAKITSLLPAKIKKLLNHPPKNVIFP
                     GFVPQETIMGAYSACDAFFFPSYEENEGIVVLEALSMEAPILIRDIPVYRGWMRSEEN
                     CLKGRDNRELSLLLDRLVADKKLAGKLTSTGKETALERDLSIIGQKLKNTYLEVLEKR
                     "
     gene            3601..4119
                     /locus_tag="PLD15_09395"
     CDS             3601..4119
                     /locus_tag="PLD15_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPB64165.1"
                     /translation="MKYFVTGEKNVGKTYLVEKIKPLVNVKGFETRFEPEGQRLFLHF
                     LNGLSFMIGERNSGRLRIIEEGFKEAARELGTMEIPESCFLIMDEIGFLEEESPEFQQ
                     AVVAAIRRSKNCICILRKGNYSFIDHLKRSMGIRSVELTVDNRDEILNGLLREVEKER
                     TDTSSPGGDEGI"
     gene            complement(4098..4174)
                     /locus_tag="PLD15_09400"
     tRNA            complement(4098..4174)
                     /locus_tag="PLD15_09400"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4138..4140),aa:Cys,seq:gca)
     gene            complement(4528..>4775)
                     /locus_tag="PLD15_09405"
     CDS             complement(4528..>4775)
                     /locus_tag="PLD15_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPB64166.1"
                     /translation="SADSLDEYSWLHLIADKSAVELYSTPLSNGMFMISKDGRIAYLD
                     DYVVQAGDGSIPVYPVFRIPTFGMTGSKWFREFVYND"
BASE COUNT         1281 a         1203 c         1178 g         1113 t
ORIGIN      
        1 gtccgctgtc ctaccaattg gacgatctcc caagacagta aagattataa agcacaagat
       61 aattggtgtc aagcgatctg gaggccttct gatgaatata gcgatgttca gtgacactta
      121 cgcgccgcag gtcaacggtg tggtgacgat gataaagatg ctcgaagaga atctccagaa
      181 aagggggcac aacgtctata tcttcacggt cgatcacccg aaggccacga gtcaggaaaa
      241 cgtgtatcgg gtaccctctc tgaggtttcc ctgggaaaaa cagcatcgaa tcggccttcc
      301 gacgaactac cgggagttga tacagatcat aacgaacctc aagatcgatc ttatacactc
      361 ccacacctcg ataatagtcg ggtatctggc caacctcgtc acctcgaaac tcggcctgcc
      421 cggtgtcacg acttaccaca ccatgatgga ggagtacgtt cactacatcc cattcatgga
      481 acccattctg agggtatata taagaaagca ggataaacgc ttctgcgaca ggaacagggc
      541 ggtgatagcc ccttccataa aaatcaagaa acttcttgag acgtacggcg tctcctcgca
      601 tatagaggta attccaaacg gagtggacct cacacccttc agaagagaga tatcagaaga
      661 agagcggagg aattttagaa accggtacaa tatcggcgag gaggatccgg tgatgatctt
      721 cgtcggcaga atgggcggcg agaagagcat agacaaactt atcgagaact tcgcccgtgt
      781 caacggggag attcccaact cccacctgct gctcgtgggc gacggcccgc tgagaacaca
      841 gctgaaagag ctttcggtcg agctgggtgt gggaagtaac ctgcatttca cgggattcct
      901 caaatggccc gccgagatct ctctggccta caagagcagc gacctcttca tcatcgcctc
      961 tcacaccgag accttcggtc tagtcactct ggaagcgatg gcctcgggcc ttccggtggc
     1021 ggcctacaaa gacgacagca tcgccaacat ggtcttcgac cgcgacaacg gtttcatgtg
     1081 tccctcgaag gacgatctct acaaagttat catagaggtt cttaaggacg attccatgag
     1141 gaagaggatg tcggaaagat cgcgcgagat ctctctggat ttctcggccg aggccaacac
     1201 cgaaaagacc ctcgacctct acgggagagt tctcaggaac gaagtttaga tttgaaacct
     1261 atctcgtggg ccgtggccag cccgccaaat attatgttca cgtagtaact gaagaaccgc
     1321 cacaccagga gcgcggcgcc tatgctttcg ccgaagaagg gccggaagaa gagcaggtac
     1381 ccgccctcgg aaacgccgct ggccccgggc gtcggtatga gcgagacgac aaggaagagg
     1441 atcaactgaa cggccgtgat ctcgaagtag ttcatctcga tccccatcga cagagccacg
     1501 aagtacggga tggagatctg gcagatcaac tgggcaaatg tggccaaact ggccagaatc
     1561 aacaccaaag gccttttcat gagttccgac atggaagagt gaaaaaagcc cagctccttc
     1621 agcgactttt cgattgccag atcgacgttt ttcactattc tcaaccagcc cagaattttc
     1681 acgatcacgc ttatcgtctt agctgtgaac ccgggactga gagaaaagag aaccagaaag
     1741 aagagcacgg cgctgttgat caaaaaaccg aagatcgaaa gaaccgccag attcgagatc
     1801 ttgcccgaaa ccaccgggta ggccccgaag atggccataa ctccgagaac cgtgatcacg
     1861 ctctggtaga taagaaacct ggaaaccagc atagccgtcg attcgccgac ggatatgttt
     1921 ctgcgctgca tgatgactat ctgcgccggc tggccgcccg aagagaacgg tgttatcccg
     1981 ttgaaaaaag ttcctatcac ggttattttg aagaggtacg tgagcgggac ggaaagcgat
     2041 ctcatcaccg cgaagacctt aagcgtcaga gcctcgaaaa accagctgca gatcgagagg
     2101 ccgaatataa gggctatcca caggggcttg gcgttcaaaa gataggagat cgactttctg
     2161 aaatcggtgg cgcccagtat aacgatgatg atcaccacac tgagcgacag cgcgatcagg
     2221 aagtttcttt tggatctctt catctttttt caaggacctc gaggtatgtg tttttgagtt
     2281 tctggccgat tatcgagaga tctctctcca gagccgtctc ctttcctgtg gaagtgagtt
     2341 tccccgcgag ttttttatcc gctaccaacc tgtccagaag gagtgaaagc tcccggttgt
     2401 ccctcccctt cagacagttc tcttcactcc tcatccagcc tctgtaaacg ggtatgtccc
     2461 ttatcaatat aggtgcctcc atcgataggg cttcgaggac gacgatacct tcgttttcct
     2521 cgtagctcgg aaagaagaag gcgtcgcagg ccgagtaggc gcccatgata gtctcctgcg
     2581 gaacgaagcc cgggaagatc acgtttttcg gaggatggtt gagtaatttc tttatctttg
     2641 cgggaagaag agaggtgatt tttgcgccga accagaagaa cctaatatcc gctttgttcg
     2701 ctgcgatatc gcagaagtct accactccct tcctcgagaa gggcaacccc acagagataa
     2761 ccagcggtcc ctctataccg tactgttttc tgaaagattc tgccaggaag gcgtttctgc
     2821 tgaacttctc tttgtctacg ccgttagaaa tgacttttat gggaagattc acaccgtaac
     2881 ccctaaccag gtccctggtg tactcgctgg gtgaaatcag gaaatcggcg gaagagtaga
     2941 gcctctttat cctgctcttt ataagagggg ccagagaatt agagaacatg aaggaattcc
     3001 tgaaatcttc gaacgtggtg tgcgtatggt agatgacagg caaaccggac ttcctggact
     3061 ttttgagcac cttctccccg cccggaccga ttgtgtttat atgagccaga tcgtaattat
     3121 cctcttcatc ggttgtgtag gcgaccccaa ccttctccag cgctatcatc tgatgcttca
     3181 aggcccttcc cacgccggac ttgctgaaga gcttttttcc ttcggaataa agcagaacct
     3241 tcatttaaac ctccaaccga tttgccaggt tcctttcaaa tgtgctcaac tttaaaaaag
     3301 tatagatcgg ttgcgaaagc ctcccggcaa attgactaat gcgaagaatc gaattatact
     3361 gaaaacgtgt cgatagtaat tactctttct caatgtgagg tgataactcg ctctcatgtt
     3421 tcaccatgca ctccaggaaa gtaactctta tccagaaaag cgcactctct tccgaaagca
     3481 cgtttatcca ccttttctct ggtattctac atcagatgcc atatttttga aacaccgtta
     3541 gtttctttcc aattgtcagc tggtccaccg gaccatagta atttgaactg ggggaatgag
     3601 atgaaatact tcgttacagg tgaaaaaaac gtgggcaaga cctatctagt agagaagata
     3661 aagcccctcg taaacgtgaa gggcttcgag acaaggttcg agccggaagg tcagaggttg
     3721 tttctccact ttctaaacgg cctttccttc atgatcggcg agagaaactc cggaaggttg
     3781 aggataatcg aagaaggctt caaagaggcg gcaagagagc tcggtaccat ggagataccg
     3841 gagagttgct tcctcatcat ggatgaaata ggttttctcg aagaggagtc tccggaattc
     3901 cagcaggccg tggtggcggc tatacgacgt tcgaaaaact gcatctgcat ccttcggaag
     3961 ggaaactact ctttcatcga ccacctcaag agatccatgg gaataaggag cgtagaattg
     4021 accgtcgata acagggacga gatactcaac ggactcctca gggaagtaga aaaagagagg
     4081 acggacacgt cctctcctgg aggcgacgaa gggatttgaa cccttgagta gagattttgc
     4141 agacctccgc ctttgacccc ttggctacgt cgccttgacg ggtttaattc tagcatgtaa
     4201 tttctatcaa ttcaacacaa aagggccgct ttcggcggcc ctcggggtgg aaaattcttc
     4261 aggagggagg ttggctccaa atattatggg cctggaacca actcgcaaca ggacgcgggg
     4321 acatcctttg caaattaatg atacagtcta ttttcaggaa acccaagccc gaatttgtcc
     4381 aacctgaagg gagcaaagtg atttagcact ctttttctac actatgaacg gctatttgaa
     4441 agtatgctcc ctacttttac gtttgccgta tctaaaaaca cttaacactg cttcttttca
     4501 ggcgcttttc tcaagctttc ggaggattca atcgttatat acgaactccc tgaaccactt
     4561 tgaacccgtc atcccgaaag tgggaattct gaacaccgga tatacgggaa tcgaaccgtc
     4621 gcccgcctgg acgacataat catccagata ggctatcctg ccatccttcg agatcatgaa
     4681 cataccgttc gacaggggtg tgctgtaaag ctccaccgcc gatttatcgg ctatcaggtg
     4741 caaccaggag tattcgtcaa gagaatctgc agaag
//
LOCUS       DAOOLY010000202         4767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21547_length_4767_cov_4.300722, whole genome shotgun sequence.
ACCESSION   DAOOLY010000202 DAOOLY010000000
VERSION     DAOOLY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21547_length_4767_cov_4.300722"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..469)
                     /locus_tag="PLD15_09410"
     CDS             complement(146..469)
                     /locus_tag="PLD15_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB64167.1"
                     /translation="MDIGKLATEMNRGFIQLLMMVMLDEPMYGYEIVRTLQERNFLID
                     ENTLYPLLRRLEEKDILASEWRVEENKPRKYYSISSEGRKVREELLKIWQEQDKLIKS
                     FVGEE"
     gene            1209..1481
                     /locus_tag="PLD15_09415"
     CDS             1209..1481
                     /locus_tag="PLD15_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64168.1"
                     /translation="MAVERDGNYSVVMIRDFGKAWKRRTARIMLIKPSVTEEELKNIT
                     LRIWEENGQDVDEMITVFFLPGMNTDSVAYSFGSCMKDGIPRISYR"
     gene            complement(1578..2108)
                     /locus_tag="PLD15_09420"
     CDS             complement(1578..2108)
                     /locus_tag="PLD15_09420"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB64169.1"
                     /translation="MSISTMTGDEGQTSLWSGERVDKDSLRVEAYGTVDELNSFLAEA
                     KHHTCEEVSRYILSVQRFLFKVGGQLASKDVQYVEPVSEEDVEKLTKSVHYFEEIVGL
                     KGFVIPGSTIASAKLDICRTIARRAERRVIALSRLEKVPDPVRKYINRLSDFLYIIAR
                     YEEFKEKKLLYKNDVE"
     assembly_gap    2351..2450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2451..4751
                     /locus_tag="PLD15_09425"
     CDS             <2451..4751
                     /locus_tag="PLD15_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HPB64170.1"
                     /translation="AGRTLTTILVVLVVSLAGISLCLVTAERARNSLSNELLNEAKLL
                     EAALNKARLKSLHGTIDDLANPAYLRLKQQLIQVKKIFPQYRFIYLMGIREDGEIYFF
                     IDSELPGSTDESLPGDPYRGATENDRKVFKEKKSTVESPIKDNWGTWASATVPVVDEL
                     SGEAIAALGIDLDIKNWNGIILEKTLPMIVLSVLLLFFSVIGLALTWWKKALPQERRE
                     KRFPRYLDAITVLFISLTITLMAAFSVNENQKSSRNEAFESLAISKATFVSQAIRDMR
                     AYELESLSLFFESSESVERREFETFVRPILSKGDMTIWGWAPAVASDERSEFESKIST
                     ELSGSFEIWEMNDQEKIVRAGERALYYPILYLEPLVDHREFLGLDLGSNAITKAVIEE
                     TVETGFMTASDPTTLLESTGEAKGVLILKPSYKEAYGAFQGFVLAFFRPEVFIKLTES
                     ISLKDGTTVMGIYLLDDVGRPVFLASTVGEHDRIDDSVSELRHYHAEEMNVIMPIFEF
                     GKVLVIVVHPSLDYERLYPLQEGRMTLAIGAIISLFLTVVVGSLNNRSYHLQREVQQR
                     TEDLRTSLDKLTRTMEGSVRALASAVDLRDPYTSGHQRRVTALAVAIAEKMGIDSERI
                     NGLRLAASVHDVGKIQVPAEILSSPRRLSALEYEMIKMHPGAGHSLFEGIEFPWPVAE
                     IIYQHHERLDGSGYPRGLSGEEIMLEARIIAVADVMEAMTSHRPYRPAPGLVEAVKEI
                     RDNSGKLYDPAVVSALIELIEEGYTF"
BASE COUNT         1158 a         1133 c         1134 g         1242 t
ORIGIN      
        1 gtatcgttta tctctccgtg ctcgcgttct gcccgctcca gtatatggct ctctatctct
       61 ctcaagatct catcgacttt ttccggctgc ctccccggta gaaaagtcct aacgttgtcc
      121 aggtactttt tcaatctttc cattttcatt cctccccaac gaagcttttt atcagtttgt
      181 cctgttcttg ccagattttc agtagttcct ctctaacttt tcttccttca ctagagattg
      241 aatagtactt cctcggtttg ttttcctcga ctctccactc gctcgccagt atatctttct
      301 cctccaggcg tctcaatagg gggtaaagag tgttctcgtc tatgaggaaa ttgcgttcct
      361 ggagtgttct cacgatttcg tacccgtaca ttggctcatc gagcataacc atcatcaaca
      421 actggatgaa tcccctgttc atttccgtgg caagctttcc tatatccata tcatcacctc
      481 ggtattgtgt tgacatgatt atactgtata taacacagta ctattatcac gaaatagtat
      541 agtatatgtt aaatgtgttt ttcaacgaag atgaagattc cagatcgcga tctcaatatg
      601 atgttgtcag agaaattgac cgtcataccg aaaatgccag tatcgttcgt aataccggtt
      661 gcctcaggta gtcagactga tgtcaagtaa tggttgttag taggttgtgg gtaagaccgg
      721 aatgccggat cggggtctgg catgacggga gggagaaaaa cagggatcct ggccctggct
      781 tagagataga ggatcccgtt ccggctctcc cgctccttaa ccaagaacga aaaacctgga
      841 tgtgcagcgt cggaactaag aaccgttctt cccgctctct caactactcg tctgagatgt
      901 cttgttgcac cgctacctca tgtgtaatac atagtttctt taacatatta actttatagt
      961 tccgattcac agccacgtat agtcagtata gaattgacag gttttgaaat ggtccgttca
     1021 gctatcttcg tgatagctga ctgaaaagga gagcaggaat cgcctttaac cggcgacgaa
     1081 tgaagcaact atgatttcca aacctaacta agatttatct ctacttaatt attgaagtat
     1141 taatggcact tggaaatttt ttgtaacatg atgtattata tatgcattac tacgatggag
     1201 ggagtatcat ggcagtcgag cgggatggaa actattctgt agttatgata cgtgattttg
     1261 gaaaggcctg gaaacggcgc acggccagaa taatgctcat aaagccttct gtcaccgaag
     1321 aggaactgaa gaatatcaca ctacgaatat gggaagaaaa cggtcaagat gtggacgaga
     1381 tgataaccgt tttctttcta cccggaatga acaccgattc ggtagcttac agtttcggtt
     1441 cctgcatgaa ggacggcata cccaggatca gttacaggta accgcgtcga atcgcctttc
     1501 gaaaaggcta taatcgattt caagaaactg atcggccatt ttttaaccgc gatatgcttg
     1561 aagcggttta ttttttatca ctcgacatcg ttcttgtaaa ggagtttttt ctctttgaat
     1621 tcctcgtatc ttgctatgat ataaagaaaa tccgagagcc tgttgatgta cttcctgacg
     1681 gggtcgggga ctttttcaag tctggagagc gctattaccc ttctctcggc tcttctggct
     1741 atcgttctgc agatatccag cttcgccgat gcgatcgtgc ttcctggaat cacgaatcct
     1801 ttgaggccaa cgatctcttc gaagtagtgg acgcttttcg taagtttttc aacgtcctcc
     1861 tcgctaaccg gctcgacata ttgaacgtcc ttagacgcca gctgtcctcc gactttgaag
     1921 aggaatctct gtacagaaag tatgtatcga ctcacctctt cacaggtgtg gtgtttcgcc
     1981 tctgcgagaa aggagttcag ttcatctaca gtcccgtagg cctccaccct cagactgtct
     2041 ttatccacgc gctcgccact ccagagactc gtctggcctt cgtcgcccgt catggtagat
     2101 atactcatcg ttatcatcct ccttccagaa aattatacct aaattcccga tgtgattatg
     2161 atcgtttcaa cacactctcg atatgtgaag atacagatcg atcttctact tacggtcatt
     2221 gcccgcaaac cccttctcgt tgctgatttt ctaaccaatc gatagctcca aacgttttat
     2281 gctggctgat ctggagaacc tgatcgattc aggctataat ttacggatga tgacatgtta
     2341 aaatcataaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctggaagaa
     2461 cccttacaac tatcttagtc gttctggtcg tttcgcttgc gggaatctct ttgtgtcttg
     2521 tcactgcgga aagggccagg aacagcctca gcaacgaatt gctgaacgag gccaaacttc
     2581 tagaagccgc gttgaacaag gctcgcctta aatctctgca cggtaccatc gatgacctcg
     2641 ccaatcccgc ctatttgagg ttgaagcaac aactaatcca ggtcaagaag atttttcccc
     2701 agtaccgctt catctacctc atgggaatta gagaggatgg agagatctac tttttcatag
     2761 attcggagct ccccggatca accgacgagt ctctgccggg tgacccatac aggggcgcca
     2821 cggaaaacga tcgaaaggtc tttaaggaaa agaaatcgac agttgagtca cctataaaag
     2881 acaattgggg cacgtgggcg agcgcaacgg ttccggtagt ggacgagctc tcaggagagg
     2941 ccatcgcagc gcttggaata gatctggata taaagaactg gaacggcatt atcctggaaa
     3001 agactcttcc gatgatagtt ctctcggtgc tgttgctgtt tttttctgtg atcgggctcg
     3061 cgcttacgtg gtggaagaag gcccttcccc aggagagacg ggagaagcgc ttcccaagat
     3121 acctcgacgc tataacggtt cttttcattt ctctgacgat aaccttaatg gccgcttttt
     3181 ctgtgaacga aaaccagaaa agctcgagaa acgaagcctt cgaaagcctg gcgataagca
     3241 aagcgacttt tgtatcgcag gcgattcgcg atatgcgcgc ttacgaacta gagagtctct
     3301 cacttttttt cgagtccagc gaaagcgtcg agcgccgtga gttcgaaacc ttcgtgcgtc
     3361 ccatcctttc taagggtgat atgaccatct ggggctgggc gccggccgtc gcctccgatg
     3421 aaaggtccga attcgagtcg aaaatctcca cagagctctc cggtagtttc gagatctggg
     3481 aaatgaacga tcaggaaaag attgtcaggg ccggtgagag ggctctttac tatccgattc
     3541 tttacctgga accgctggtg gatcaccggg aatttctggg actggatttg ggttcgaacg
     3601 cgataacgaa ggcggttatt gaagagacgg ttgagaccgg gttcatgacg gcctccgatc
     3661 ctacgactct gcttgagagt acgggcgaag caaaaggtgt cttgattctg aaaccctcat
     3721 ataaagaagc ttatggcgcc tttcagggat tcgtgctggc tttcttccgg cccgaagtct
     3781 tcattaaatt aaccgagtcg atctccctta aagacggaac gactgtgatg gggatttatt
     3841 tgctcgatga tgtaggcaga ccggtctttc tggcatcgac ggttggcgaa cacgatagaa
     3901 tagacgactc ggtgagcgag ttgcgccact accacgcgga ggaaatgaac gtgatcatgc
     3961 cgatctttga attcgggaag gtcctcgtga tcgttgtgca tcccagcctc gattacgaaa
     4021 ggctgtatcc ccttcaggaa ggccggatga cgctggctat cggagcgatc atctcgctgt
     4081 tcctgacagt ggtggtaggt tcgctaaaca accgcagtta tcatctccaa agagaggtcc
     4141 agcagaggac ggaagacctg cgaacgagcc tggataaact cacccggacc atggaaggtt
     4201 cggtcagggc gctggcctcg gcggtggacc ttcgcgatcc ttacacgtct ggccaccagc
     4261 gccgcgtgac cgcgctggcg gtagctatcg ccgaaaaaat gggtatcgac agcgaaagga
     4321 tcaacgggtt gcgcctagcg gcgtcggttc acgatgtcgg aaagatccag gtaccggctg
     4381 aaatactgag ctcccccaga agacttagcg ccctcgagta cgaaatgata aagatgcacc
     4441 cgggcgctgg acattccctg tttgagggga tcgaatttcc ctggcctgta gccgagataa
     4501 tatatcagca tcacgagaga ctcgatggca gcgggtatcc ccggggtcta tcaggcgaag
     4561 aaatcatgct tgaggccaga ataatcgccg ttgccgatgt catggaggcc atgacctccc
     4621 acagacctta ccgccccgct ccgggactgg tagaggccgt caaggaaata agagacaatt
     4681 cgggaaagct ttacgacccg gcggtggttt cggccctcat agagctcatt gaagagggat
     4741 acaccttcta gaggaaacag actttcc
//
LOCUS       DAOOLY010000203         4693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_21981_length_4693_cov_5.497197, whole genome shotgun sequence.
ACCESSION   DAOOLY010000203 DAOOLY010000000
VERSION     DAOOLY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21981_length_4693_cov_5.497197"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PLD15_09430"
     CDS             complement(<1..855)
                     /locus_tag="PLD15_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64171.1"
                     /translation="MRKLTAVLIILLVAMSFASAKLVIWSSENQIPALEKLAADFESD
                     YGIQVEIQQVNFGDIKSKFLTAAPAGEGPDIIVGAHDWVGELAINGLIEPIPFLPDRE
                     QYYEVALDALNYGGKLYGVPYTIEAIAIIYNKDLVFDVPKTLTELEQLALEASDDEVV
                     GFIYTAGDFYHSAPFILGRGGYIFKETSAGQDVKDIGLSNQGAVAGGNLIKSWFDKGL
                     IPGGPNYNLMDSLFKDGLAAFIVNGIWATPSYRDAGIDYAIIPFSEIAFDDGTTPRPF
                     VGVQGFMIN"
     gene            complement(1065..2459)
                     /locus_tag="PLD15_09435"
     CDS             complement(1065..2459)
                     /locus_tag="PLD15_09435"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPB64172.1"
                     /translation="MRKVCFVDTTMRDAHQSLLATRVTTGELLEVAALVDRVGYHAVE
                     VWGGATFDSCVRYLNENPWERLDALKGEFKNTKLQMLLRGQNLLGYRNYADDVVDLFV
                     ETMSRHGIDIVRVFDALNDFRNLEKSTQAIKKYDMHLQIAMSYTTSPVHSIEYFVELS
                     DRAIEFGADSLCIKDMAGLLKPGTAYELVSEIKKRHAIPLEVHSHFTTGLADMTYLKS
                     AEAGADYLDTATSSMAFGTSQPGVESLWTALADIGLAEKPDYRLLEEINGYFEKVRRN
                     HKGTDAGFTINTSVLENQIPGGMYSNLINQLKAQNMQERFPEVLEEVPRVREELGYPP
                     LVTPTSQIVGVQAVLNVITGERYKMITKEVEKYIKGFYGKPPADVSSELKKRVLGDGE
                     PISFRPGDAIEPEVEKGRKELGLLARNDEDLLIYLLLGEVGKKYLYEKYREDLKIDFD
                     IARDYQDGMTVYPV"
     gene            2508..4388
                     /gene="mnmG"
                     /locus_tag="PLD15_09440"
     CDS             2508..4388
                     /gene="mnmG"
                     /locus_tag="PLD15_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPB64173.1"
                     /translation="MCSNKRDYEFDIVVIGAGHAGIEAALSSSRMGLRTLVLNINLDT
                     VGWAPCNPAIGGPAKGVVVREIDALGGEMAKTTDETSLNIRVLNTSKGAAVRALRAQI
                     DKYEYSKLMKRKLEEQNDLSLQSGIVTDIEAENGRIVAVTTHFGLKYSAKAVIVTTGT
                     FLGGKIFIGPREFPAGRLGEIPATELSRSLIKLGFNVRRFKTGTPARIAGRSINFERM
                     ERQDTSSSPLAFSHFSEPFVLENNHPCWLTRTNDKTHEIIKRDLHFSPLYGDVKLINS
                     RGPRYCPSIEDKVIKFSSKDSHQLFVEPESASSNEYYLNGLSTSLPYETQLEMIRTIP
                     GLEKAEIVRPAYAVEYDYIDPTQLYPTLESKIVENLYFAGQVNGTSGYEEAAGQGLLA
                     GINASAKLLGLRQMIVGRSEAYIGVMIDDLVTRGVDEPYRLLTSRAEYRLLLRHDNAS
                     LRLVEYGYRYGLIPKWFFEKICRLKEEVAKQIDRLSKISIKPSGRLNDLLVKAGSTAI
                     DTTVKFISLLRRAGIGYNDLEEFDPQPLKDQDLIEQVDIETRYGGYVERLKEEINRLG
                     ELENESIPRTLNYDEVANLSTEGREKLKKTAPESLAQAMRIPGITPSDIMNLSFHLRM
                     KK"
     gene            complement(4407..4580)
                     /locus_tag="PLD15_09445"
     CDS             complement(4407..4580)
                     /locus_tag="PLD15_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64174.1"
                     /translation="MLDSSLAGLQTSVDPVQEMTVRYPRILVIKAAFALLKDGKSIEH
                     RDLEKTLQLFLSK"
BASE COUNT         1213 a         1174 c         1145 g         1161 t
ORIGIN      
        1 gtttatcatg aacccctgaa ctccgacgaa gggtctcggc gtggtgccgt cgtcgaaggc
       61 aatttcgctg aaaggaatta tagcatagtc gattccggcg tctctgtagc tcggtgtagc
      121 ccatataccg ttgacgatga aggccgccag tccatccttg aacagagaat ccatgaggtt
      181 gtagttcggt ccgccgggga tgagcccctt gtcgaaccag gacttaatca aattaccacc
      241 ggcgaccgca ccctggttgc taaggccgat atctttgaca tcctgtccgg cagatgtttc
      301 cttgaatata tatccgcctc ttcccagtat gaagggagcc gagtgataga agtctccggc
      361 tgtgtagata aagccgacca cttcgtcatc gctggcttca agggcgagct gttctagctc
      421 tgtcagtgtt ttgggtacgt cgaaaaccag gtctttgttg taaatgatcg ctatggcttc
      481 gatcgtgtat ggaactccgt aaagtttccc gccgtaattt aaagcatcca gagcaacttc
      541 gtaatactgc tctctgtcgg gaaggaaggg aatcggttcg ataagaccgt tgatggccag
      601 ttctcctacc cagtcgtggg caccgactat tatgtccggt ccctctccgg ccggagcggc
      661 cgttagaaac ttcgatttaa tgtcgccgaa attcacctgt tgtatctcga cctgaatacc
      721 gtagtcgctt tcaaaatctg cagcaagttt ttcgagtgcg ggtatctgat tttccgaaga
      781 ccagatgacg agctttgcac tggcaaaaga catagctaca agcagaatga taagaactgc
      841 cgtgagtttt ctcattcttt agaacctcct ttgtggttat tggcgtatcg ccttacaaaa
      901 attttaccag aaatccagtc ttctaattat taatgttcac actgtggcag tgagaaaaat
      961 cttacccacg ggcaattaga ggcgtataag tggaaatact gtatgttttc tcttctttag
     1021 aaaatcgaat atggtttcat ttgtttcgct ttcaaaaccg tactttaaac tggataaacc
     1081 gtcataccgt cctgataatc cctggcaata tcgaaatcta tcttcaggtc ttccctgtac
     1141 ttctcgtaaa gatacttctt gccgacctct ccgagcagga gatatatcaa gagatcctcg
     1201 tcgttcctgg caagcagacc cagttctttc ctgcccttct ccacctccgg ttctatcgca
     1261 tcgcccggtc tgaaagatat gggttcacca tctccgagaa ctctcttctt caattcggag
     1321 gagacatctg cgggtggttt gccgtaaaat cctttgatat atttctccac ctctttggtt
     1381 atcatcttgt acctctcgcc tgttataacg ttgagaaccg cctgaacacc gacgatctgc
     1441 gaggtggggg taaccagtgg cggatagccg agctcctctc tgactctggg aacctcttca
     1501 agaacctcgg gaaatctttc ctgcatgttc tgcgctttca gctgatttat caggttcgaa
     1561 tacatccctc cggggatctg gttttcaagc acggaagtgt tgatggtgaa tccggcgtcg
     1621 gtacctttat ggtttctcct aaccttttca aagtaaccgt ttatctcttc cagcaatcta
     1681 taatccggct tttctgccag cccgatgtct gccagtgccg tccagagact ctcaacaccg
     1741 ggttgcgagg tgccgaaggc catagaggag gttgccgtgt ctagatagtc ggctccggcc
     1801 tccgcactct tgaggtaagt catgtcggcc aggccggtag tgaaatggga atgcacttca
     1861 aggggaatgg catgccgctt ctttatctcc gagacgagct cgtatgccgt accgggtttt
     1921 agaaggcccg ccatgtcctt tatacatagc gaatcggcac cgaattcgat cgctctgtcg
     1981 gagagctcga cgaaatactc gatactgtgc accgggctag tggtatagga catggctatc
     2041 tggagatgca tgtcgtactt cttgatggcc tgagtcgatt tctcgaggtt tctgaagtcg
     2101 ttcagcgcat cgaagaccct gacgatatct atgccgtgcc tggacatcgt ttcgacgaag
     2161 agatcgacta catcatccgc gtagttcctg tatccgagaa ggttttgacc cctgagaagc
     2221 atctggagtt ttgtgttctt gaactcaccc ttcagagcgt ccaatctttc ccagggattc
     2281 tcgttcaaat acctcacgca ggaatcaaag gtcgcgcctc cccagacctc gacggcgtgg
     2341 taacccactc tatctaccag ggcagctacc tctagaagct cacccgtcgt aaccctcgta
     2401 gccagtaaag attgatgcgc atctcgcatc gtagtatcaa cgaaacagac ttttctcaaa
     2461 tcgatgcccc ctctctgtgt acaatactat gatagaggag tgatgttatg tgttcaaata
     2521 aaagagatta cgaatttgac atagttgtca tcggagccgg tcacgccggt atagaggcgg
     2581 cactttcaag ctcccgtatg ggtctgagaa cactggttct gaacataaat ctcgatacgg
     2641 tcggatgggc accctgcaac cccgccatcg gcggccccgc aaagggggtc gtcgttagag
     2701 agatagacgc cctgggtgga gagatggcca aaactaccga cgaaacatct ttgaacataa
     2761 gagttttgaa tacaagcaaa ggtgcggccg tcagggccct gagggcacag atagataaat
     2821 acgaatacag taaactaatg aagagaaagc ttgaggaaca aaacgattta tcgctccaga
     2881 gcggaatagt tacggacatc gaagccgaaa acgggcggat cgtcgccgta acgacgcact
     2941 tcggtctaaa atactcggct aaagctgtga tcgtcaccac cgggaccttc ctgggaggga
     3001 agatcttcat aggccccagg gaatttccgg ccggaagact cggagagatc cccgcgacag
     3061 aattatcccg ttcgcttata aagctgggat tcaacgtaag acgcttcaaa acaggtactc
     3121 cagcgaggat cgccggccgg tccataaatt tcgaaaggat ggaacgtcag gacacgtcaa
     3181 gctctccgct ggccttttca cacttcagcg agccctttgt gctcgaaaac aatcaccctt
     3241 gctggctaac gagaaccaac gataagaccc acgaaataat aaaaagagac ctccacttct
     3301 ctccactgta tggagacgta aagcttataa attcacgcgg accgcgttac tgtccgtcaa
     3361 tagaggacaa ggtaataaaa ttttcctcga aagacagcca tcaactcttt gtcgaaccgg
     3421 agagcgcttc gagcaacgag tattatctga atggcctgtc cacgagtctg ccttatgaaa
     3481 cacagctgga gatgatcaga accatccccg gtcttgaaaa ggccgagatt gtcaggccgg
     3541 cctacgccgt agaatacgat tacatagatc cgacgcaact gtacccgacg cttgaaagca
     3601 agattgtcga gaatctctac ttcgccggtc aggtcaacgg gacgagcggc tacgaagagg
     3661 ccgccggcca gggacttctg gcagggataa acgcctccgc aaagctgctc ggtctccggc
     3721 aaatgatcgt ggggcgatcg gaggcttaca tcggcgtcat gatagacgac cttgtcacca
     3781 ggggagtgga cgaaccttac agattgctca cctccagggc cgagtacaga ctcctgttga
     3841 gacacgacaa cgccagtctc agattggtcg agtacggata caggtatgga ctgataccga
     3901 aatggttttt cgaaaagatt tgcagattga aggaagaagt cgcgaagcag atcgacagac
     3961 tctcgaaaat ttctataaaa ccttcgggaa gactcaacga tcttctcgtt aaagccggaa
     4021 gcacagcgat cgatacaacc gtaaagttca tctccctgct gaggagggcc ggcatcggat
     4081 acaacgatct cgaggaattc gatcctcagc cacttaagga tcaagatttg atcgaacagg
     4141 tcgatataga aaccaggtac ggtggatatg tggaaaggtt gaaagaggag atcaacaggc
     4201 tgggcgagct ggaaaacgag tccattccta gaaccctgaa ctacgatgaa gtggccaatc
     4261 tctcgaccga gggaagggaa aaactcaaaa aaacggctcc ggaatctctc gcgcaggcga
     4321 tgagaatacc cggtatcact ccttcggata taatgaacct ctcgtttcat ctgaggatga
     4381 aaaaatgaaa gacgctctat acacgatcat tttgaaagaa acaactgcag agttttctca
     4441 agatctctat gttcaataga ctttccatct ttgagaagag cgaatgcagc ctttatgaca
     4501 aggattcttg gatacctgac tgtcatctct tgaacgggat cgacagatgt ctgaagtcct
     4561 gcaagactgc tatcaagcat attatcatct cctgaacctc ttgacataag ccaagaggga
     4621 tattataatc aattctgtgt tcgggctcgt agctcagttt ggttagagct tccggctcat
     4681 aaccgggcgg tcg
//
LOCUS       DAOOLY010000204         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_22028_length_4686_cov_3.705247, whole genome shotgun sequence.
ACCESSION   DAOOLY010000204 DAOOLY010000000
VERSION     DAOOLY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22028_length_4686_cov_3.705247"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLD15_09450"
     CDS             <1..287
                     /locus_tag="PLD15_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB64175.1"
                     /translation="LQKKLSDLEWENESLRSELQESKEVIENLIRKLESLSEEETPAT
                     SELVVYRQDVNTTVDAFKKALKSAGQGFAQFIHWLFDTESEKPVEENREE"
     gene            597..1226
                     /locus_tag="PLD15_09455"
     CDS             597..1226
                     /locus_tag="PLD15_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPB64176.1"
                     /translation="MNSELDFADIFARIAQLEEIYEELNASINRTMLQTGLRCLPGCR
                     SCCETPSSNIEVSILEFLPLSMNLWQNDKAGQLLEILELKTEDDPCVLLQNDASLLPE
                     GGCSHYGFRPLMCRLFGFSAMIGRDAFPVPVICKLVKVNYPDIMRKVVDSVNGGLDIP
                     VFSNFARRVRGIDPYLAERVYPINQALKKAIEFIGLRWHFGSLGYDKPA"
     gene            1331..2572
                     /locus_tag="PLD15_09460"
     CDS             1331..2572
                     /locus_tag="PLD15_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64177.1"
                     /translation="MRKFILFLLFMTISIALFGQLGLRTLTPEATQTTPAVSPLETLT
                     LRTVDPLLQSFRDGWKEPEDPTWTSFIVGNRELKLPQHFSISTQKYDVNYDVRIYDSE
                     GRLFGWLFYYQLESYDLQELLNTVVSSVYGDKATGEKPFEEKRSFDNGLAAYLASLKI
                     TGQTVDFPSVIVYSPKESLDVTSAGSVAIFFFEPSNFTGPALDMAKSWIAGVVGSFVY
                     QTEAIVEPVVTPEIIEPPVVEVTVPDDPFIRIIASLLAGEDVEIENIDSWKDVEGEYF
                     GFAMPPEFSVEFLMGDGFEVADLGIGGMILAKFMVGEFYESAVTTEILGEIVGTYLGA
                     FGGYSIAASHTIKIEGESSINLYALDFSGQFCWIALFSESPNPETFGPGEFFVFLGLV
                     DPTMKDQLAGYYAGMLATLNF"
     gene            complement(2643..3962)
                     /gene="xylA"
                     /locus_tag="PLD15_09465"
     CDS             complement(2643..3962)
                     /gene="xylA"
                     /locus_tag="PLD15_09465"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPB64178.1"
                     /translation="MIFKDIDRIRYMGPDNRDPLAFSFYDAEEEVLGVKMGDYLRFSV
                     AFWHTFKGDGKDMFGDPNVRRGWSTIKDEMTLAKIKIDGLFELCEKLDIKYFCFHDRD
                     IAPEGETLREANRKLDEIVVHIKDHLKDGKVRVLWGTANLFSNPRFVHGAATSCSAEV
                     FAYAAAQVKKALEITRELGGENYVFWGGREGYETLLNTDMSLEIDNLGRFMRMAVDYA
                     KEIGFTGQFLIEPKPMEPTKHQYDFDVANSLAFLRKYGLENHFKFNIEANHATLAGHT
                     FQHELRYARINSMLGSVDANQGDTLLGWDTDQFPTDLYTTTLAMYEIALNGGLKPGGL
                     NFDAKVRRGSMEEKNLFAAHIAGMDTFALGLKATALLLEEGSIERLLQERYSSFSKGV
                     GKEIVDGTASFKSLESYILDMKDISPEPSNQEYLERLVNWAIANAAR"
     gene            complement(3976..>4686)
                     /locus_tag="PLD15_09470"
     CDS             complement(3976..>4686)
                     /locus_tag="PLD15_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB64179.1"
                     /translation="TSGTVVAVTNSAVPDTTGGIHLFRHVSSDSFYHMAVILSATNSL
                     NWFKERFAGEIELGEIEAIVSGTPVGSNGIIFLPYLNGERTPHRDPNARGTLFGFSSF
                     HGKGDVFRSIYEGVAFALREGADLIKRLGTSIERIRIVGGGSRSDTWCQIIADNLGRE
                     VWSPRVDEGAAYGSARLAAMAMGLESDSWIKLDRCFTPNCEGKDVYDAIFEIYRGLYS
                     SLKENFLVLGELQKRLVK"
BASE COUNT         1165 a         1139 c         1118 g         1264 t
ORIGIN      
        1 tacttcagaa aaagctctcc gatctagaat gggaaaacga atctctgaga agcgaacttc
       61 aagaaagcaa agaagtgata gaaaacctta taagaaagct ggaatcatta tcggaggagg
      121 aaacgcctgc aacttcggag ctcgtcgtgt acagacagga tgtgaatacg acggtagacg
      181 ccttcaaaaa ggctttgaaa tctgccggac agggttttgc tcagttcatt cactggcttt
      241 tcgacaccga gtcggagaaa ccagtggaag aaaaccggga agagtgaagt ataagttcaa
      301 gggcctcctc ttatttgatg atatagaaga caacaactct tcaaagcaaa gagtaaccat
      361 cttcgtatta catgacagcc ccttgtttgt gtaactgttg cgagattctt taagaatacg
      421 aactacggga attaatctgc gaagattagt atttgaaagt gaagcattac ggagatttac
      481 acctatatcc gggtgtggta gccgctgaat gtatctaaac ggctttcttg aataatcaag
      541 tgtatttatt tcgaaacccg gcacgttgat cttccggctc tccaggagtg tgatctatga
      601 acagtgaact ggacttcgca gacatatttg caagaatcgc ccagctcgaa gagatctacg
      661 aagagctgaa cgccagcata aacagaacga tgcttcaaac cggtctcagg tgtcttcccg
      721 gctgcagaag ttgttgcgag acgccttctt cgaacatcga ggtttctatc cttgagtttc
      781 ttccgctctc tatgaactta tggcagaacg ataaagccgg acaattgttg gaaattctgg
      841 aattaaagac agaagatgat ccctgtgttt tgctgcaaaa cgatgcctca ttgcttccag
      901 aagggggttg cagccactat ggattcagac cgttgatgtg ccggcttttt ggcttttccg
      961 caatgattgg aagagacgca tttccggtgc ccgtgatctg caaactcgtg aaagtcaact
     1021 acccggatat aatgcggaag gtcgttgata gtgtcaacgg cggcctcgat atacctgtgt
     1081 tttccaactt cgcaaggcgg gtaaggggta ttgatccgta tctggctgaa agagtatatc
     1141 ccatcaatca ggctctgaag aaggctattg aattcatagg attgagatgg cattttggtt
     1201 cgctggggta cgataaacct gcctgatcta cagatgagtc actttccact gtgcatatat
     1261 ctataagccg tggtagtaca attttcttta gaccagagta atacctggaa tctcacttgg
     1321 aggtgtttat atgaggaaat tcattttatt ccttctcttt atgactattt caatcgcgct
     1381 ttttggacag ctgggtctaa gaaccttgac cccggaggca acgcagacca caccggcagt
     1441 ttctccgctt gagacactca ccctgagaac ggtcgatcct ctgctgcaat ccttcagaga
     1501 tggctggaaa gaaccggaag acccgacatg gacctctttt atcgtcggca atcgagagtt
     1561 gaagctgccc cagcattttt cgatatcgac tcaaaaatac gatgtgaatt acgatgtgag
     1621 aatatacgat tcagaagggc ggcttttcgg atggcttttc tactatcagc tcgaaagcta
     1681 tgaccttcag gagttgctga acactgttgt ttcatctgtg tatggagata aagctaccgg
     1741 agagaagcct ttcgaagaga agaggtcttt cgacaacggg ctggcagcct atctggcttc
     1801 gttaaaaata acggggcaaa ccgttgattt tccctcggtg atcgtttact ctccgaagga
     1861 gagccttgat gttacaagcg ccggctccgt agccattttc ttcttcgaac catcgaattt
     1921 caccgggccg gctctggaca tggccaaatc ctggatagcc ggtgtggtgg gaagcttcgt
     1981 ttaccagact gaggcgatag tcgaaccggt agttacaccc gagataatcg aaccccctgt
     2041 cgtggaagtt accgtacccg acgacccgtt cataagaatc atcgccagcc tgctcgccgg
     2101 ggaagatgta gaaattgaga acatagattc ctggaaagac gttgagggcg agtattttgg
     2161 cttcgcaatg ccaccggaat tttccgttga attcttgatg ggcgatgggt tcgaagttgc
     2221 agacctgggt attggtggaa tgatcctggc gaaattcatg gtaggggagt tttatgagtc
     2281 tgccgtgacg accgaaatcc tcggcgaaat agtcggtacc tatctcggtg ccttcggagg
     2341 gtacagcatc gctgccagtc acacaataaa gatagaaggc gagtcgtcga tcaatcttta
     2401 cgcactcgat ttttcaggtc agttctgctg gatagcgctg ttctcggaat cgccaaatcc
     2461 cgaaacgttt ggcccgggtg agttttttgt cttcttgggt ctggtagacc cgacgatgaa
     2521 ggaccagctt gccgggtact acgccggcat gcttgccaca ctcaacttct gaatatcgaa
     2581 gaaccagccg atgaagggcc atgccttttc aggcatggcc ctacttttaa gtcggttgcg
     2641 ctttatctgg cagcgtttgc tattgcccag tttacaagtc tttcaaggta ttcctgattc
     2701 gaaggttccg gtgatatatc tttcatgtct aggatataac tctcaagact tttaaaagat
     2761 gccgttccat ccacaatctc ttttccgact cctttagaaa agctcgaata tctctcctgc
     2821 aacagccttt cgatagagcc ttcttcaagg agcagagcgg ttgccttcag accaagagcg
     2881 aaggtgtcca ttcctgcaat gtgggcggcg aagaggttct tctcctccat tgagcctcta
     2941 cggaccttag catcgaaatt caatcctccg ggttttaaac cgccgttcag agctatttcg
     3001 tacatggcta gcgttgtggt gtacaaatcg gtcgggaact ggtcggtatc ccatccgaga
     3061 agcgtatcac cctggtttgc gtcaacactg ccaagcatac tgttgatcct tgcgtacctc
     3121 aattcgtgct ggaaggtatg acctgccaga gtagcgtggt tggcctctat gttgaatttg
     3181 aagtggtttt ccagtccgta ctttctgagg aaagcgagtg aattggccac atcgaaatcg
     3241 tactgatgct tcgttggctc cataggcttt ggttcgatta aaaactggcc ggtgaaaccg
     3301 atctccttgg cgtaatcgac ggccattctc atgaacctgc ccaggttgtc tatctcaagt
     3361 gacatatctg tgttgaggag agtttcgtac ccctctctac caccccagaa aacgtagttt
     3421 tctccgccca gctctctggt aatttcgagg gccttcttga cctgtgctgc tgcgtaagcg
     3481 aaaacttctg cactacacga agttgcagct ccatgaacga atcgcgggtt tgaaaagaga
     3541 ttggcggtcc cccaaaggac gcgaactttc ccatctttca gatggtcctt tatatgcacg
     3601 actatctcat caagtttcct gttggcctct cgcagagttt caccctccgg agcgatgtct
     3661 ctatcgtgga aacagaagta ctttatatcc agcttttcac agagttcgaa gagtccgtcg
     3721 attttgatct tcgcaagagt catttcatcc tttatcgtgc tccatccccg tcttacgttg
     3781 ggatctccga acatatcctt tccatcgcct ttgaaggtat gccagaaagc tacagagaaa
     3841 cggagatagt cgcccatttt gaccccgagg acttcttctt ccgcgtcgta aaagctgaaa
     3901 gccagaggat ctctgttgtc cggtcccata tacctaattc tgtcgatatc cttgaagatc
     3961 atgttgtccc ccttttcatt tcaccaatct tttttgtagc tcgcccaaaa ccaaaaaatt
     4021 ctcttttaag ctgctgtata gacccctgta tatctcaaag atcgcatcgt aaacgtcttt
     4081 tccttcacag ttcggcgtaa agcacctatc gagctttatc caggaatccg attccaaccc
     4141 catagccatg gctgcgagtc tggcagagcc ataggcggca ccttcatcga cccgaggaga
     4201 ccagacctct cttcccaggt tgtcggcaat tatctggcac catgtatcac ttctcgaacc
     4261 gccacccact attctgatcc tttctataga cgttcccaat ctctttatga gatcagctcc
     4321 ttccctaagc gcgaaggcga ctccttcgta aatgctcctg aacacgtctc ccttgccgtg
     4381 gaaggaagaa aaaccaaaaa gcgtacctct ggcgttcggg tcacgatgtg gagtcctctc
     4441 gccgttgaga taaggtagaa agataattcc gtttgaacct acgggcgtac cggaaacgat
     4501 agcctcgatc tcgccgagct cgatttcacc ggcgaacctt tccttgaacc agttcagcga
     4561 gttggttgcc gaaagtatga ccgccatgtg gtagaaggaa tcactggaaa cgtgcctgaa
     4621 gaggtgaatg cctccggtgg tatcgggtac cgcactgttc gtaaccgcca ccaccgtgcc
     4681 cgaagt
//
LOCUS       DAOOLY010000205         4655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_22211_length_4655_cov_3.425435, whole genome shotgun sequence.
ACCESSION   DAOOLY010000205 DAOOLY010000000
VERSION     DAOOLY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22211_length_4655_cov_3.425435"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="PLD15_09475"
     CDS             complement(<1..1865)
                     /locus_tag="PLD15_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPB64180.1"
                     /translation="MHLKKTIMIVLLALLPILVTAYKIRPGDVLYVSVYGSAELSGEI
                     VVGPDGTASIPPLGSVAVLGKTLEEAESLLSGKYLSGGILATRPPVTVSVKSYAPFMF
                     YVLGEVNKPGAIEWRKDSLTVSQMIALAGGLKDGADLSASFLVESNGNRKHIDLSSFL
                     VGGEASSITLAEGTTLFIPAGAKAWIRSIGEFRSPALIRYNPGLTLTQVVAQSGGLTE
                     NADGNEILIIGEGVQGGKRVVDLNSVLSGVTVDPLIPAGSIVVANDSSVKSVKLLGEV
                     GSPSSISFRDGLTLIQAIGQAGGVKSGAGSQLFIIRGELSEPLRIELIPLLRGEIKDV
                     PLKPGDTIFVPRESEKFVYISSPTFGGKVEFGVDESITLANALVKVDLYNPAGDESLI
                     LVEPSGERVEVRLNEDRILQSGSMILVSGGVKSVYIAGEVNSPGMMQFGNFDEITLAK
                     VISRAGGYTEKAGGVEIISPGGKSVYSTEQALRSDVKLSVQSIVLVGRVEERYVYVVA
                     PGSGGRIDFKENESFTLKNLLARLNLLNLNSSKEIEVIEPSGKVNAVAASRLKESDLP
                     LQAGAIVIVPEMSSWVYVFGEVSRPGKIEVDGEDLTLTRVLSSSGGYTGVADISSV"
     gene            complement(1893..2243)
                     /locus_tag="PLD15_09480"
     CDS             complement(1893..2243)
                     /locus_tag="PLD15_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB64181.1"
                     /translation="MKKILIVEDEKNMRFLLKEELKEEGYEVTVASNAQEAEKILDGG
                     VRFDLVTIDIEMEGMNGLELAGLMRKRLPDLKIVLLTAYTHYKHDLSSWAADAYIVKS
                     PDFSELKALIRELI"
     gene            complement(2221..>4655)
                     /locus_tag="PLD15_09485"
     CDS             complement(2221..>4655)
                     /locus_tag="PLD15_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB64182.1"
                     /translation="RDLLFLNHEGDPLGLLSLPRGSSIASTLKGWISELEMALHYSLT
                     RNYEQEGYRRLKLVSRLTSVLESETMIERLIYSSMEVAREMLRVRWIFFLEQSGSKFA
                     LKAAIGEGRSPLKSIEFQLSVQQLDYLTSGRLFVSAKKSDISSVFFDDGAAVGSFIVV
                     PLLSEGDFIGFFLAADREEVEGEFRPYRHLDEEDVRLLDDVGRRMGVAISKIRLTSRL
                     ELEVRKLKQLGKKHEESISEQKDQLFKLGALHKISQAMRKSLDYERIVSILLVGATHS
                     AGLRFDRAFFLKKNSRMALLTVEAKVARDEGGEFERAVYGDLSRYLQYVSLERHFVTD
                     KDNHISVSYLGNMILERVVNRRKIVHVTPDMGRFRSEELSMLNKIVGTQDYLVAPVSG
                     EKDVQGVIVVDKALTGNKISNADTEILGLLADSAGVALELTGNYERLLEITDSLEKER
                     NLSNYYRKFVSSILQSLESSIVVCSPFGEITEVNRTAELLLDAKREELIGKNVEILTG
                     KLEGVLELLFDVLRIGETITLSDQRFESLGERYFDVKITPLRQDSGGHLAGVIISMDE
                     VTRRRKLEQEFKSREKLAALGEMSARVAHEIRNPITIIGGFIKRMAATNDREKMGEYA
                     TILREELGRLDGIVREVLEISRARTSIQEEGFDLLALTKEVLDGFQERAQEDNVKLIL
                     ESKEEKLEYYADKNRIKQVIINLVQNAIEASEHYGEVLVKIDKDPRNVVFSVWNSGRV
                     IPQEILKRIFEPFFTTKTLGTGLGLSICRKIVQEHGGEIEAESGEKGTVFTFRLPVQK
                     IGRFDREKNTYR"
BASE COUNT         1029 a         1361 c         1025 g         1240 t
ORIGIN      
        1 acgctcgata tgtcggccac ccccgtgtat ccaccgcttg agctaagcac cctggtgagt
       61 gtgagatctt ctccgtcgac ttcgatcttt cccggtctgg acacctctcc gaagacgtaa
      121 acccacgaac tcatctcggg cacgatgacg atcgcccctg cctgaagcgg cagatcactc
      181 tctttcaacc gcgaggcggc caccgcgttg accttccccg acggctctat aacttcgatc
      241 tcctttgagg agttcaggtt gagaaggttg agcctggcca ggagattctt cagagtgaaa
      301 ctttcattct ccttgaaatc tatcctccca ccgctccccg gagccacgac atacacgtat
      361 ctctcttcga ccctccccac caggactatc gattgcacag agagcttcac atcgctcctc
      421 aaggcctgtt cagtcgaata gaccgacttt ccaccgggag agattatctc cacgccaccg
      481 gccttttcgg tgtagccgcc ggccctcgat atcactttgg ccagagtgat ttcgtcgaaa
      541 tttccgaact gcatcatacc cggagagttg acctcgccgg cgatataaac gcttttaaca
      601 ccgccgctaa cgagtatcat agaacccgat tgaaggatcc tgtcctcgtt gagacgaact
      661 tctacacgct ctccagatgg ttctaccagt attaagctct cgtcgccggc cgggttgtac
      721 aaatccacct tcaccagagc gttggccagg gtgatcgact catcgacgcc gaactccacc
      781 tttcctccga aggtcggcga ggagatgtaa acaaacttct cgctttcacg cggaacgaag
      841 atggtgtctc ccggtttgag tggcacatcc ttgatctcac ctctcaatag agggatcaat
      901 tcgattctca aaggttcaga gagctcgccc cttattataa acagctggct cccggcgccg
      961 gatttgacac cgccggcctg cccaatggct tgaatcaggg tcagtccatc cctgaaactt
     1021 atcgacgacg gcgaacccac ctcgcccagc aacttcaccg atttaacgga ggagtcgttg
     1081 gcgaccacta tactacccgc tgggatcaac gggtcaactg tgacaccgga gagcactgag
     1141 ttgagatcga ccaccctttt tccaccctga acgccctctc ctattatcaa gatctcattt
     1201 ccgtcggcgt tttccgtcag tccgcccgac tgggcaacca cctgggtgag tgtcaacccc
     1261 gggttgtacc ttatcagggc aggggatctg aactctccga tgctcctgat ccaggctttg
     1321 gcaccggcag gtatgaaaag cgtcgtaccc tcggccagtg taatcgacga tgcctctccg
     1381 ccgaccaaaa aagaagagag gtctatatgc tttctatttc cgttgctctc gaccaggaag
     1441 gaggcggaaa ggtctgcccc gtccttcagc cctccggcca gagctatcat ctgcgatacg
     1501 gtaagactgt ccttcctcca ttctattgca ccaggcttgt taacctctcc gagaacgtag
     1561 aacatgaaag gcgcgtaaga ttttaccgag accgtcaccg gtggcctcgt cgccagaatt
     1621 cctccactca ggtatttacc agataaaagc gactcagcct cttcgagcgt cttgcccagc
     1681 accgccacgc tgcccagcgg tggtatcgac gcggtgccgt ccggccccac gacaatttct
     1741 cccgacagct ctgcggaacc atagaccgaa acataaagaa catcacccgg tctgattttg
     1801 taggccgtaa ctagaatggg caggagtgcc agaagcacaa tcatgatcgt cttttttaag
     1861 tgcattttat gtccccctct ctttaaattc tcctatatca gttctcttat caacgctttc
     1921 aattcggaaa aatccggtga tttcacgatg taagcgtccg ctgcccagga agagagatcg
     1981 tgtttgtaat gcgtgtaggc cgtgagaaga actatcttca agtcgggcaa ccgctttctc
     2041 atgaggccgg ccagttcgag gccgttcatc ccttccatct ctatatctat cgtaacgaga
     2101 tcgaagcgaa ctccgccgtc gaggattttc tcggcctcct gggcgttgct tgcgacggtc
     2161 acttcgtaac cttcttcttt tagctcttcc tttaagagaa atctcatgtt tttctcatct
     2221 tcaacgataa gtattttttt cacgatcaaa cctcccaatc ttttgaaccg gcaatctgaa
     2281 ggtgaacaca gtgcctttct ccccgctttc ggcttcaatt tcgcctccgt gctcctgaac
     2341 tatcttcctg catatcgaaa ggcccagacc ggtacccaga gttttagttg tgaagaaagg
     2401 ttcgaatatt ctcttcagaa tctcctgagg aatgactctg ccactattcc ataccgaaaa
     2461 gacaacgttt ctaggatctt tatctatctt gacaagcacc tctccgtaat gttcagatgc
     2521 ctcaatcgcg ttctgaacaa ggttgattat aacctgtttg attctatttt tatctgcgta
     2581 atattccagc ttctcttctt tcgattccag aattaactta acattatctt cctgagctct
     2641 ttcttgaaaa ccgtccagca cttctttcgt aagtgccaga aggtcgaatc cctcttcctg
     2701 gatactggtt cttgctctgg agatttccag cacttccctc actataccgt cgagtcttcc
     2761 gagttcttct ctcaatattg tcgcatattc tcccatcttt tccctatcgt ttgtagcggc
     2821 cattcttttg atgaatccac ctattatcgt gatgggattc ctgatttcgt gggcgactct
     2881 ggccgacatc tcaccgaggg cggctagctt ttctctgctc ttgaattcct gttcaagctt
     2941 tctgcgcctg gtcacttcgt ccatggagat aatcacgccg gcgagatgtc ctccgctgtc
     3001 ctgtctgaga ggagtgatct ttacgtcgaa gtatctttcg ccgagacttt cgaatctctg
     3061 atcgctgagc gttatagttt caccgattct taaaacgtcg aagagcaact ccaggacgcc
     3121 ttccagtttt ccggtcagaa tctcgacatt cttaccgatc aactcctcgc gctttgcatc
     3181 tagcaagagt tcggcggtgc ggttgacctc ggttatctct ccgaacggag aacagaccac
     3241 aatcgacgac tcgagagact gaagaatgct ggacacgaat tttcggtagt aattggaaag
     3301 gtttctctcc ttctcgaggc tgtcggttat ctccagcagc ctctcgtaat tccccgtcag
     3361 ttctagcgcc acaccggcgc tatcggccag tagacccagt atttccgtgt cggcgttact
     3421 tattttgttt ccggtcaggg ccttatcgac cactatgaca ccctgaacat ccttttctcc
     3481 ggaaaccggc gccaccaaat aatcctgcgt tccgactatt ttgttgagca tggagagctc
     3541 ttcgcttctg aaccggccca tgtcgggtgt tacatggaca atctttcgac ggttgacgac
     3601 tctctcgaga atcatattcc caagatatga aacggaaatg tggttatctt tatcggtaac
     3661 gaagtgacgt tctagcgaaa catactgcag atatctcgaa agatctccgt acactgctct
     3721 ttcgaactct ccgccttcgt ctctcgcgac ctttgcctcc acagtcagaa gagccatgcg
     3781 cgagtttttc tttagaaaga aagccctgtc gaacctcagg cccgcagaat gtgtcgctcc
     3841 gaccaggaga atactcacta tcctctcata atctaggctc ttcctcatag cctgagatat
     3901 cttgtggagc gcgcccagct tgaacagctg atccttttgc tcgcttatcg attcttcgtg
     3961 tttcttgcca agctgcttca gttttctgac ttccagttcc agccttgaag tcagccggat
     4021 cttgcttatt gcgacgccca ttctccggcc gacatcatcg agcaatctca cgtcttcctc
     4081 atccagatgt ctgtaaggcc tgaattcccc ttccacttcc tcccggtcgg cggccagaaa
     4141 gaagcctatg aaatcgcctt cggagagtag aggcacgacg atgaaagatc ccaccgctgc
     4201 cccgtcgtcg aagaaaaccg aggatatatc tgattttttc gccgaaacga acagccttcc
     4261 tgaagtcaga tagtccagct gctggacgga aagctggaac tcgatgcttt tcaggggact
     4321 cctgccttcc ccgatcgcgg ccttcagagc gaatttactt ccagattgct ccagaaagaa
     4381 gatccacctc acccttagca tttctctggc gacctccata gaagagtaga tcagacgctc
     4441 tatcatcgtt tcgctctcaa gtactgacgt gaggcgcgat accagcttca gtcttctgta
     4501 accttcctgt tcgtagttac gtgtcagcga atagtggagc gccatctcca gttcggaaat
     4561 ccatcctttc agggtgctgg ctatagacga gccacgcgga agtgagagca gcccgagcgg
     4621 atcgccctcg tggttcaaaa agagtagatc cctcg
//
LOCUS       DAOOLY010000206         4623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_22425_length_4623_cov_4.967601, whole genome shotgun sequence.
ACCESSION   DAOOLY010000206 DAOOLY010000000
VERSION     DAOOLY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22425_length_4623_cov_4.967601"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PLD15_09490"
     CDS             <1..1482
                     /locus_tag="PLD15_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPB64183.1"
                     /translation="KEVLFSVIPIAVLVLVLNFLFVSLSWWLIIRFVIGSFLIVVGMS
                     LFLFGVSIGISPIGHFMGNFIAKTNKVWIIAALGFVLGFFISIAEPALHIHASQVELV
                     SSGLLSSGSIVITASIGIAVFLAFGLVRIVYNVALQKLMTVFYGIILVLSLFSSGEFL
                     AISFDASGATTGAMTVPFILTLALGISILKRDSRSSENDSFGLLGVASAGAIISVLLI
                     GIVSNNTSMESAPDYLVSESTAILGYFFGPVPGIAREVLLSLLPILLTFLLLQLTSLR
                     LSRRSVRQITFGLFFTFLGMVIFLVGVNAGFMDVGREIGYGIASTGNKAFVVIVGFIL
                     GLVTILAEPSVYVLTHQIEDVTSGAVKRRIVLVALSAGVSLAVGFSMVRIVVPGIQLW
                     HFLLPGYVISIFLSFFSPKLFVGIAFDAGGVASGPMIGTFVLAFAQGAVRAVDGANIL
                     LDAFGLISMVAMTPIIALQILGLIYKMKIRKRGVESSDDSRES"
     gene            1460..2128
                     /locus_tag="PLD15_09495"
     CDS             1460..2128
                     /locus_tag="PLD15_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPB64184.1"
                     /translation="MTTLENRNVFRLIYVVVNFGRGSDVLRIAKEKGISGGTILLGRG
                     TIRNGILNFFAFYESRKEIVMMVADGETAQTALEALNNEMRFDRPNHGIAFTTGICDV
                     EGSRELELSLPEKERGEEKTMRHLITVIVDKGNAEKVIDAATEAGSKGGTIVNARGSG
                     IHERSKLFAMEIEPEKEIVMIVSKRDTTQKIIANIRETLRIDEPGKGIIFTQLVDEAY
                     GLLE"
     gene            2579..4210
                     /locus_tag="PLD15_09500"
     CDS             2579..4210
                     /locus_tag="PLD15_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64185.1"
                     /translation="MKDFFIIVKWRSKENARLVTRQGKKTILVSSLAIIFLVMVSVPL
                     IFYMRRYFIPNFSRIAGLSDQLRELTGIDLKMIVITFFSAIIFSILLGSDLPIVISNL
                     FFSDRVKLLLQMPVKKSTIAKVQISEVLTTGGLPVLLFVPVFLAALSGLGFSGSRFWL
                     ALVLLLVFVIDILCITSVISFAVVFLSRGRFLKVLSSIMTMVTLFTFIFTLRFMDFSA
                     IDLARPDRVAEQFGTLQGVITAPYLPWTPFVRAIVGEIGDVVLFLLYSLAIVAILEFL
                     ELFLYPGVVQKLLTQPTGSTKDFRRASRGRSHSFSGILKKDFLLLIREPKLTFAFLYP
                     SLFVPVVVMVNPALLRSFGILQLLGLIVFLFSNYSTVSSTALFAFERQLGDNGFLFPV
                     KRVGVVISKASIVTFLYSLIIFLVGLYVVSKASVYRNFMFTFLFFMLPTIFSLSLLGG
                     YLEKSFGTRDTSNVFKALTLAGAVISFVLSTLLPVFTTLPLAMYTTGGIASFLGFLNL
                     PAADPWTWLFGMAIPFLLWAGIIWASLKSLCYNFV"
     gene            4234..>4623
                     /locus_tag="PLD15_09505"
     CDS             4234..>4623
                     /locus_tag="PLD15_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB64186.1"
                     /translation="MKAQDVKYLSRKIMEAGEIPLLWGHFGVGKTDLAREIAEETGRK
                     LVILVISQMEPGDLIGMPSRDGERTLFLRPDWWPTDGNVILMIDEVNRAHRSIRNAIM
                     QLLIDKRIHNHFLPEGSWIIAAANPPDE"
BASE COUNT         1098 a         1077 c         1143 g         1305 t
ORIGIN      
        1 aaggaagttc tgttttccgt tatcccgatt gcagtactgg ttctggtact gaacttcctg
       61 ttcgtctcgt tgagttggtg gctcataatt agatttgtta taggttcttt cttgatcgtt
      121 gtcgggatgt ctttatttct tttcggagtc agcataggaa taagcccaat cgggcacttc
      181 atgggtaatt tcatcgccaa gacaaacaag gtatggatta tagccgcttt ggggtttgta
      241 cttggcttct tcatatcgat tgccgaaccg gccttacaca ttcatgcctc tcaagtcgag
      301 ctagtgtctt caggcttgct ttctagcggg agcatcgtga tcaccgcatc aattggtatt
      361 gcagtctttc tcgcttttgg acttgtgaga atcgtctata acgtcgctct gcaaaaactg
      421 atgactgtgt tttatggaat aatactcgtt ctttcgctct tctcttcggg agagttcctg
      481 gcgatctcct ttgatgcttc tggagccact accggggcaa tgaccgtacc cttcattctc
      541 acgcttgcat taggtatttc tatactcaaa agagacagta gatcgtctga aaacgacagc
      601 tttggactcc tgggagttgc ctccgccgga gcgatcatct cagtactgct tatcggtata
      661 gtatccaaca ataccagcat ggaatctgcc ccggattacc tcgtatcaga atcaactgcg
      721 attctgggat acttcttcgg gccggtaccc ggtattgcca gagaagtact gctttcgttg
      781 ctgcctatac tgttgacttt cttgctgctt cagctgacct ccttgaggtt atccaggaga
      841 tcggtgagac agataacgtt cggactgttc ttcacttttc ttggtatggt gattttcctg
      901 gtcggggtca atgccgggtt catggatgtt ggcagagaaa tcggatacgg aatagcatcg
      961 acgggcaaca aggcatttgt tgtaatcgtc ggcttcatcc tgggccttgt gacaatcctc
     1021 gcagaacctt cggtttacgt cctcactcat cagatagagg atgttacaag cggcgccgtg
     1081 aagagaagaa tagtccttgt cgccctttcg gcgggcgtca gtctggcagt tggcttttcc
     1141 atggtgagaa tcgtggtccc aggcatccag ctgtggcatt ttcttcttcc gggctatgtg
     1201 atttccatat tcctctcatt tttctctccg aagcttttcg tcggaatagc tttcgatgcg
     1261 ggaggtgttg cttctggtcc gatgatagga acgtttgttc tcgcttttgc tcaaggcgcc
     1321 gtcagagccg ttgacggagc gaacattctc ctggatgcct tcggtcttat atccatggtt
     1381 gcaatgacac ccattatcgc tctacagata ctggggttaa tatacaagat gaagataaga
     1441 aaaagaggtg ttgaatccag tgacgactct cgagaatcgt aatgttttca gactcatata
     1501 cgtagttgtg aacttcggcc ggggaagcga tgttcttcga atagcaaagg aaaaagggat
     1561 aagcggtggc acgatacttc tggggagagg taccatcaga aatgggatac tgaacttttt
     1621 cgcattttac gagagccgaa aggaaatcgt tatgatggtt gctgacggtg aaacagcgca
     1681 gacagcactc gaagctctca ataacgaaat gagattcgac cggcctaatc acggaattgc
     1741 tttcactact ggcatctgcg acgtggaagg gtcaagagag cttgagctct ccttgcccga
     1801 aaaagaaaga ggtgaagaaa agacaatgcg tcacttgatt accgtaatag ttgacaaagg
     1861 aaatgctgaa aaagttatcg acgctgccac tgaggcggga tctaagggtg gaacgatagt
     1921 aaacgcaaga ggttccggaa ttcacgaaag aagcaagtta ttcgcgatgg aaatagagcc
     1981 tgagaaggaa attgttatga tcgtttcaaa gagagacaca actcaaaaga tcatagccaa
     2041 tataagagag actctcagaa tagacgagcc gggtaaagga atcatcttca ctcagctggt
     2101 cgatgaagct tacggactgc tcgagtaaag cctttgttca agaaaaagac acgagatgat
     2161 ccgcacgtta ggggccgtcc ttggttcttc gtcccagatc atggacccgt ccttagaaag
     2221 aacggaaggg cgagaccccc gtattagagc cctgaatagg atcatttcag ggcaggctat
     2281 tacgggatga cagtgcttgg gcattcacgg gattatagtc ccttcccgtc atgccggact
     2341 ccgatccggc atccttcgaa agagcgaaag aactgttgtt cgacgatgct gcgtgtccag
     2401 gttcttggtt aaggagtgcg agaagaggag agagatgccg aaaatcgggt acaacagacg
     2461 ccaggagcac gtcagtcacc attttcgctc ttacccacaa cccacaacag ttcatagagc
     2521 gatactaatt ttgagaacaa taaataaatg ctgagcctat ttatggcaga atatagttat
     2581 gaaagatttc tttatcatag ttaagtggag aagcaaagaa aacgctagac tggtcacccg
     2641 ccagggaaag aaaaccatac tcgtctcttc tctggcgatt atctttttgg ttatggtcag
     2701 tgttccgctc attttctata tgaggagata cttcatcccc aacttttcga ggattgccgg
     2761 actgtccgat cagttgaggg aactgaccgg catagatctg aaaatgatag tcataacgtt
     2821 tttttctgcg atcatcttct cgatccttct gggcagcgat ctccccatag tcatttcgaa
     2881 tctcttcttt tccgacagag ttaaactact actacagatg cccgtcaaga aatcgaccat
     2941 tgccaaggta cagatttccg aagtcctcac caccggcggt ttgccggttc tgctgtttgt
     3001 gccggtcttt ctggcagccc tgagcggatt gggtttttcc gggtcccggt tctggttggc
     3061 gttggttctg ctcctcgtct tcgttataga tatcctgtgc attacaagcg tgatatcttt
     3121 cgcggtagtt ttcctctcca ggggcagatt cctcaaagtt ctctcctcga taatgactat
     3181 ggtgacactc ttcacgttca tctttacact taggttcatg gacttctcgg ccatagatct
     3241 cgccagaccc gaccgtgtgg cagagcagtt tggcacactt cagggagtga taacggcccc
     3301 ttacctgccc tggacaccct tcgtcagggc aattgtagga gagatcggcg atgtggttct
     3361 attcttgctt tactctcttg cgatagtggc gatcctcgaa tttcttgagc tttttctata
     3421 ccctggagtc gttcaaaagc tgctgacgca gccgacaggt tctacaaaag acttcagaag
     3481 agctagtcgt ggccgatcgc attccttctc cggaatcttg aaaaaggatt ttctccttct
     3541 gattagagag ccaaaactca ctttcgcctt tctgtatccc tctctcttcg tacctgtggt
     3601 cgttatggtc aaccccgcac tcctgagaag tttcggaata ctgcaattac tgggattgat
     3661 cgtcttcctc ttcagtaact attctaccgt ctcttcaacg gcacttttcg ccttcgaaag
     3721 acagctgggc gataatggct ttctcttccc ggtgaaaagg gtcggtgtgg ttatatcgaa
     3781 agcctcgata gtcacatttc tttactcgct catcatattc ctggtcgggc tttatgtggt
     3841 atcgaaagcc tcggtttaca ggaatttcat gttcactttt ctcttcttca tgcttcccac
     3901 gatattctcg ctgtcgctgc tgggcggcta tctcgaaaag agcttcggta ccagggatac
     3961 gagtaacgtt ttcaaggctc tcacacttgc cggcgctgtg atctcgttcg ttctatcgac
     4021 tcttctacca gtcttcacga cgcttcccct ggctatgtac actaccggtg gcatagcctc
     4081 atttctgggt tttttgaatc ttccagccgc cgacccgtgg acctggctct tcggaatggc
     4141 cataccgttt ctgttgtggg ccggtatcat ctgggcatcg ttaaagtcac tgtgctataa
     4201 ttttgtctga atgaggtttt tggagggatg ttgttgaaag ctcaagacgt taagtatctt
     4261 agcaggaaga tcatggaggc cggagagata ccgctccttt gggggcattt cggagtgggc
     4321 aagactgacc ttgccagaga gatagcagaa gagaccggca ggaaactggt gatacttgta
     4381 atctcccaga tggaaccggg agatctaata gggatgcctt ccagagacgg ggaacggacg
     4441 ctgtttttga gaccggactg gtggccgacc gatggaaacg tgatacttat gatcgacgag
     4501 gtaaacaggg cccacagatc aataaggaac gccataatgc agcttctcat agacaagcga
     4561 atacataacc attttcttcc agagggtagc tggatcatcg cggctgccaa cccgccagac
     4621 gag
//
LOCUS       DAOOLY010000207         4604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_22551_length_4604_cov_4.575951, whole genome shotgun sequence.
ACCESSION   DAOOLY010000207 DAOOLY010000000
VERSION     DAOOLY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22551_length_4604_cov_4.575951"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLD15_09510"
     CDS             <1..656
                     /locus_tag="PLD15_09510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64187.1"
                     /translation="TGFTLKWYAELFRQSDIWAAFLNTIIIAVTSSVAATAIGTFAAL
                     ALYWYQSRLKSYLGILVYTPLIIPDILMGVSMLVFFVAVGLPLGLFTIFLAHVTFCVS
                     YVTVTVMTRLEDFDYSVVEAARDLGARSRQVFTKVVAPIIMPGIMAGFLLSLTLSIDD
                     FVITFFVAGPGSTTLPLRIFSMIKFGVSPVINALSTLLLIGTLFVSFFGSKFRKLIF"
     gene            672..1685
                     /locus_tag="PLD15_09515"
     CDS             672..1685
                     /locus_tag="PLD15_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64188.1"
                     /translation="MKRAAIFLFLVLLAVLSLGKGNLILYGWSDYIPEEVIENFSKEY
                     DVRVTYDTYDSNETMFAKLKAGARGYDLAMPSADYTSIMIKENMLLKIDISKIDNLVN
                     IDPDIIAQMYYDPNNEYSIPYMVGTTGIAVNTLYLKDYPKSWEIFQIPQLRGKMTLLD
                     DMREVFGAALKYLGYSVNTTDETQLREAKNLILEWKKNIAKFDNELFAKGVISGEFWV
                     VQGYGENIFYEATEEELENIDFFIPQEGSTMWIDSFVILKGARNIDNAHLFINYILRP
                     EVSAKISDYLLLPSPNVPARELMEEEPVYTAQEMENTELIKDLGRDLRLYNDLWNEIR
                     FGL"
     gene            complement(1856..2572)
                     /locus_tag="PLD15_09520"
     CDS             complement(1856..2572)
                     /locus_tag="PLD15_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPB64189.1"
                     /translation="MITVVLAAGQGKRLRSSVPKPLVKIKGKTMIEHVIEKAFYATEN
                     RKAVVVINPDFEGDFLSWLKVPVTFAYQSIPKGTADALARTLKLIEDSEDILVMYADL
                     VLISRDSLKSLVSLHTERNCDITFLSGIAEKKYPYALVERDGSGRVIAFQEKKVPDFD
                     PPWEFYIGPIVLKKSVVEAYIEKLSPNESTGEIYIADIVSLAIADNRNICGFVTKDEK
                     EFLGVNTPEDLKAVEETIEQ"
     gene            3001..>4604
                     /locus_tag="PLD15_09525"
     CDS             3001..>4604
                     /locus_tag="PLD15_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB64190.1"
                     /translation="MRSRLTPFLIAVGSTLFMLVVILMMVENRAIKTEKQLASSTKLF
                     VEETIKTVLVGYFHRDCMLKSIKDGDIESANYHLGNLVEGNDSLSGVRVVKNGTTFIE
                     LGETIAYTDSVVVQGERPYMIFYDKGSVMAATAISDSHGAQNLDGSYALIGIKLSGIV
                     DLLTDEKYALSDEADTKIYGSLGIKVARGLNLGNAFSLVFAFVISYLFMIFIQAKTER
                     SVAVAKNREQTFLLNSIPSMIWCLKDSRTFGMVNRQFAHFFGKRSEEIENHVIEEILP
                     ASEVESCIESNEEVMRSKTPVSFKQSSTNHAGEKRTIAITKIPGISESGEVDSIVCTA
                     EDVTDKEAAESRLRLIQFALDNVNEEAFWISKEGDILYVNIAACKALGYDYEELVNMK
                     VCDVDPTEEAKHRNMNWEQVKKSGQDMIETYHRRKDGTVFPVEVNRNHLIYEGQEYEF
                     SFARDISERRSAQSRLEREKRRIERLHETALAMERCREKREVFELIIQAARSILDFDI
                     CFVSIAENDHFSIKISSNLSPEDPTT"
BASE COUNT         1270 a         1083 c         1138 g         1113 t
ORIGIN      
        1 ggacgggctt caccctgaag tggtacgcgg aactcttccg gcaatcggat atttgggcgg
       61 cattcttaaa cacgatcata atcgccgtca cctcctcggt cgcggctaca gcgatcggca
      121 ccttcgcagc attggcgctc tactggtacc agtcgcgtct caagagctat ctcggaattc
      181 tcgtttatac acccctgata attcccgata tcttgatggg tgtatccatg ctggttttct
      241 ttgtggcagt ggggttacct ctaggtctgt tcacaatatt tctggcccat gtgacgttct
      301 gtgtatcgta tgtcaccgtg acggtaatga cgagactcga agacttcgac tactctgtgg
      361 tcgaggctgc gagggatctg ggagctcgca gccgccaggt ctttacaaaa gtggttgcac
      421 cgataattat gcctggaatc atggccggtt tccttctatc gctgacgctc tccatagatg
      481 attttgtcat aacctttttc gtggccggcc ccggttcgac cacgctgccg ttgaggatat
      541 tttcgatgat taaattcgga gtctctccgg tgataaacgc tctttcgacc ctgctcctga
      601 taggaacgct tttcgtctcc tttttcggga gcaagttcag aaaactaatc ttttaggagg
      661 tgggaacgta gatgaaaaga gctgcgatct ttcttttcct ggttttactg gccgtacttt
      721 ctctgggtaa gggcaatctg atcctttacg gctggtcgga ttacattccc gaagaggtaa
      781 tagagaattt ctcgaaggaa tacgatgtaa gagtgacata cgacacttac gattccaacg
      841 aaacgatgtt cgccaaactc aaagccgggg cgaggggtta cgacctggcg atgccctcgg
      901 ccgactacac aagtatcatg atcaaagaga atatgctgtt gaagatcgat atatcgaaaa
      961 tcgacaacct ggtcaacatc gatcccgaca taatcgcgca gatgtactac gatccgaaca
     1021 acgagtacag cataccttac atggtcggca cgaccggaat agccgtcaac accctctacc
     1081 tcaaggacta cccgaaatca tgggagatct tccagatacc tcaattgagg ggcaagatga
     1141 ctcttctgga cgatatgaga gaggtcttcg gtgccgcgtt gaaatacctt gggtattccg
     1201 ttaatacgac cgacgagact cagctcagag aagcgaagaa tctcattctg gaatggaaga
     1261 agaacatagc caaattcgac aacgaactct tcgccaaagg ggttatttcc ggagagttct
     1321 gggtggttca aggatacggc gagaacatat tctatgaggc taccgaagag gagctcgaaa
     1381 atatcgactt tttcatccct caagagggga gcaccatgtg gatagacagc tttgtaatcc
     1441 tcaaaggtgc aaggaacata gacaacgcgc acctgttcat aaactatata ctaaggcccg
     1501 aagtgtcggc gaagatctcc gattatctcc ttctcccctc tccaaacgta ccggccagag
     1561 aactgatgga agaggaaccg gtttacactg cccaggaaat ggaaaacacc gagctgatca
     1621 aggaccttgg aagggatttg aggctgtaca acgacctctg gaacgagata aggttcggcc
     1681 tctgagttcg aacagccaaa taaaatcgaa tagaaaaaga taagaccggg agagactccc
     1741 ggtcttcgta actcaggggt aatcgtgctg ctgacaatcg ctttgcattt caaaggggtt
     1801 gtgcctgtgg tttctctggt ctctgattat gagacttgac cccccaaaaa aaggttcatt
     1861 gttcaattgt ttcttcgacg gcttttaagt cctccggggt attcactccg agaaactcct
     1921 tttcgtcttt tgtcacaaag ccgcatatat tcctgttgtc agcgattgcc agcgatacta
     1981 tgtcggctat gtaaatttct cctgtacttt cgttcggaga taacttttct atatacgcct
     2041 ccaccacgga cttcttcaga actatcggac cgatgtagaa ctcccagggc ggatcgaaat
     2101 cgggaacctt cttctcctgg aaagctatca ctctgccact cccgtctctt tcgaccagag
     2161 cataggggta tttcttctcc gcaattccgg aaaggaaagt gatatcgcag tttctctcag
     2221 tgtgcagcga gaccaaggat ttcaaagaat ctctcgagat aagcaccagg tcggcataca
     2281 taaccaggat gtcctcacta tcctctatca atttcagagt ccgggcaagc gcatcggccg
     2341 ttcccttcgg aatgctctgg taagcgaaag taacgggaac tttcagccag gaaaggaaat
     2401 ctccttcgaa atcgggatta ataactacta ctgccttgcg gttttccgtg gcatagaaag
     2461 ccttctcaat aacgtgttcg atcatagttt tgcccttgat cttcactagc ggtttcggca
     2521 cggaggatct caggcgtttt ccctgtccgg cggcaagaac aaccgttatc aattgaaaca
     2581 cctctttctg cacatcctta tccagaatga taacacgttc gaaagacaga aaaggtgaaa
     2641 ttgactcgta cttctttggt taaggaccac gagatgagga gggaagagag agagaagaga
     2701 gagaagagag agaaaagaga gggaaagagg gcgagacgcc ttctcgtgag cgaagcgaac
     2761 tctcgacgct ctttctcgtg tttttcactc tcacccacaa cctctcctag aaagctgtct
     2821 ggtcgccgta agcaactgat gatctcgaag cgatatgggc aaggaaaccc tgagttgcca
     2881 gcgatgagtt gaaccgtttc ccggtagtaa taaatttgat ttcttccaaa tctccggcaa
     2941 aaatatatca agtgttaaaa tctgtatagg ttcacacggc ttcttaatga ggggattatc
     3001 ttgaggtcga gactaacgcc gtttttgata gccgttggca gcaccctatt catgctggtt
     3061 gtaatactga tgatggtgga gaacagggcg atcaaaacgg agaaacagct cgccagctct
     3121 accaagctct tcgtcgagga aaccattaag accgttcttg tcggttactt tcaccgcgat
     3181 tgcatgttga aatcaatcaa agacggagac atcgaatcgg ccaattatca cctcggaaac
     3241 ctcgtggaag gcaacgattc cctgtcagga gttagggttg taaagaacgg aacgactttc
     3301 atcgaactgg gtgagacaat agcttacacc gacagcgtgg ttgtgcaggg tgagaggcct
     3361 tacatgatct tctacgacaa gggttccgta atggctgcga ctgcgattag cgatagccac
     3421 ggagcacaaa acctggacgg ctcatacgca ctcatcggaa taaaactgtc cggtatcgtc
     3481 gatcttctaa ccgatgagaa gtacgccctg tcagatgagg ccgatacgaa gatatacgga
     3541 agtctcggaa taaaggtcgc gagaggtctg aatttaggaa atgctttttc tcttgtcttt
     3601 gcgtttgtaa tcagctatct atttatgatt tttatccagg caaagacaga aagaagtgtc
     3661 gcggttgcaa agaacagaga acagaccttc cttctcaaca gcataccgtc gatgatatgg
     3721 tgtctgaagg acagcaggac cttcggaatg gtgaatcgtc agtttgcaca ctttttcggc
     3781 aagcgatctg aagagataga gaaccatgtt atcgaagaga tcctgccggc aagtgaagtc
     3841 gaaagttgca tcgagagcaa cgaagaagtt atgagaagca agacccctgt ctccttcaag
     3901 cagagctcaa cgaaccacgc cggtgagaag agaaccatag ccatcaccaa gattcccgga
     3961 atcagcgaga gcggagaagt ggactctata gtctgcactg ccgaagatgt gaccgacaag
     4021 gaagctgccg aaagtaggct cagattgatt cagttcgctc tggacaacgt caacgaagaa
     4081 gccttctgga tctccaaaga gggcgatata ctctacgtta atatagcagc gtgcaaggcc
     4141 ctgggatatg actacgagga actcgtaaac atgaaagtct gcgatgtcga tccgaccgaa
     4201 gaggcaaagc acaggaacat gaactgggaa caggtcaaaa agagcggtca ggatatgata
     4261 gagacttacc atagacggaa agacggcact gtctttcccg tagaagtgaa cagaaatcat
     4321 ctgatttacg aaggccagga gtacgaattc agcttcgcca gagacatatc tgaacggcga
     4381 agtgcacaga gcagactgga aagagaaaag cggaggatcg aaaggctcca cgaaacggct
     4441 ctggccatgg agagatgcag agaaaagagg gaggtcttcg aacttataat tcaggcagcc
     4501 agaagcatcc tagatttcga tatttgcttc gtttcgatcg ccgagaacga tcacttctcc
     4561 atcaagatct cgtccaacct cagtcccgaa gacccaacga ccat
//
LOCUS       DAOOLY010000208         4586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_22674_length_4586_cov_4.216288, whole genome shotgun sequence.
ACCESSION   DAOOLY010000208 DAOOLY010000000
VERSION     DAOOLY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22674_length_4586_cov_4.216288"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLD15_09530"
     CDS             complement(<1..625)
                     /locus_tag="PLD15_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPB64191.1"
                     /translation="MKAKIPPSSVESEEAVLGSILIDPDVVPDIMELLTSQDFYLRKH
                     QLIFATMERLFDEGNPVDIVSVTERLRTSGSLEEIGGEVELAKLADLVPTSANFSYYA
                     KTVKEKSLLRSLITAASSIVESAYENNDTDEILDNAEKAIFQITESQVSKTYQPIGKI
                     MHEVFQNLEKLKENALSGRITGVATGYRKLDETTTGFRPSDLVIVAAR"
     gene            complement(600..1769)
                     /locus_tag="PLD15_09535"
     CDS             complement(600..1769)
                     /locus_tag="PLD15_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPB64192.1"
                     /translation="MLEAIREIRLGDSTTLVGICYGNVYVDGEGGQLGDRGSVDDHKI
                     LSVKKDGELCSIEIEGFIHKKAGETVDIEIDIRRRRDISVQHTAQHLLSAVFDRELGA
                     RTVGFQMGEEYSTIDLTISILTDEMQELVEKISNSMIAENRGVGIEMMPGDTAGKLDL
                     RKAISEKVVESGKDVRIVTIEGIDKSACGGFHVESTGDLRVLKIIKREKVKGDLIRIF
                     FVAGERALLDYGRKHGVLVRASSTLSCGFLDLPGRVDSLVEEVKEAKASLRKLSERVA
                     ETVARELNLTDGETIFVEDSEEVVSLIPKYLEKKEYLFAGRFGDRVLLSSKGFDCGTI
                     VTYLRDKLEVKGGAGKERGQFIFSGDSSLLKEHIHEAAVNLQKAGEKSESKDSTQ"
     gene            1881..2918
                     /locus_tag="PLD15_09540"
     CDS             1881..2918
                     /locus_tag="PLD15_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64193.1"
                     /translation="MQERPILSVKNLSTHFKMVEGVVKAVQDATFDLYRDEVLGIVGE
                     TGSGKSVTVKAIAGMIDNPGFIAGGEVLFLTDEFSKSGEKEYIDLAKLPKESFSRIRG
                     KHIGMIFQDPMTSLDPMYTIGNQMIETIVHHKGVTVEEARERAVKLLEQVGIPKPSER
                     INDYPFQLSGGQRQRVVIAIALSCDPDILIADEPSTALDVTVQAQILELMKDLQEQFN
                     SSMMFITHDMAVIAEIATKITVMYGSYQMEMGQSKDIFEHPMNPYTYALLECIPRLDV
                     KQDRLLPIPGQPPVMLNPPDLCPFLPRCSRASEKCYKDLPQLEQTGDSHFVRCWNPIA
                     GKVKLVKEAQG"
     gene            2915..4081
                     /locus_tag="PLD15_09545"
     CDS             2915..4081
                     /locus_tag="PLD15_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64194.1"
                     /translation="MKLLEVKNLKKYFPIKQGFLVERVVGFVKAVDDVSFSVDRGKTI
                     GIVGESGCGKTTIGKSIIRLHEVTDGEMLIDEEDTTFYFMKRKRALQYLKSKYFKDQD
                     FDGDGSGLETYKKKIHDVFVKSDRNPSKTLNTLFENLEEKKRLLRRKAQIVFQDPMSS
                     LNPRMTVGQMLAEPLLFHKMAEKMDDAIAMVKDLLVKVGLKAYHVDRYPHQFSGGQRQ
                     RIAVARAISVNPDLIVLDEPTSALDVSVQAQIVNLFEELQKQLNAGYVFISHNLALVR
                     FICQEVSVMYLGRIVEQGDSESIFAAPLHPYTKALLAAAPVPDPNKKRNRKDLIGGQV
                     PSPINRPSGCFFNPRCKYRMDICTREYPPMFKVDQKHFVACHLYSTSQEQGGEK"
     gene            4081..>4586
                     /locus_tag="PLD15_09550"
     CDS             4081..>4586
                     /locus_tag="PLD15_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64195.1"
                     /translation="MKKFLVLVSVLLIAGLALSATEYKVENFNGKTGGTFYYWGLGDP
                     KSFNIDWAQETSSTVPLGFIHATLLEADEGGMPTESGLAKEWWFSDDGLTFFVKIREG
                     LKWSDGAPFTIDDVYWTFVDVSLNPEQTANGNGSYMDSNDQLPVVEIVDSTTISFTWT
                     VPMVTALR"
BASE COUNT         1178 a         1142 c         1096 g         1170 t
ORIGIN      
        1 gacgcgccgc aactatcaca agatcggaag gtctgaaacc ggtagtcgtc tcgtccagtt
       61 ttctgtaacc ggtggcgact ccggttatcc ttccactgag cgcgttttct ttgagtttct
      121 cgaggttttg aaagacttcg tgcattatct tgcctatagg ttgataagtc ttggaaacct
      181 gtgactccgt gatctggaat atagccttct cggcgttatc gagaatctcg tcggtatcat
      241 tgttttcgta agcgctttcc acgatagaac tggccgcggt tataagtgat ctcaaaagcg
      301 atttttcttt gaccgtcttc gcgtaatagg agaagttggc cgaggtcggc acgaggtcgg
      361 ccagtttggc gagttcaact tctccaccta tctcttcgag cgatccggat gttctcaacc
      421 tctcggtgac ggagacgata tcgacggggt tcccctcgtc gaagagcctt tccatcgtcg
      481 cgaaaatcag ctggtgcttt ctcaaataga agtcctgact tgtgagaagc tccatgatat
      541 ctggaacgac atcgggatcg atcaatatgc tcccgagaac tgcctcttca gattcgacgc
      601 tactgggtgg aatctttgct ttcactcttt tcaccagcct tttgcagatt tacagcggct
      661 tcatgtatgt gttctttcaa aagcgaggag tcaccggaaa agataaactg tcccctttcc
      721 tttcctgcgc cccctttgac ttccagtttg tctctgagat aagtcacgat cgttccgcag
      781 tcgaaacctt tgctagaaag caagaccctg tctccaaacc ttcccgcgaa gaggtactcc
      841 ttcttttcga gatatttggg aatcaatgag acgacctctt cgctatcttc gacgaaaata
      901 gtttctccat cggtcaggtt caattcccgt gctaccgtct ccgcaactct ttcagagagt
      961 tttctaaggg acgctttcgc ttctttgact tcctccacca gcgagtcgac acggcctgga
     1021 agatcgagaa agccgcagct cagagtcgaa gaggccctga cgagcacacc gtgctttctg
     1081 ccgtaatcca gcagggccct ctcgccggca acgaagaaga tccttatcag atcgcccttg
     1141 accttttctc gtttgattat tttcaaaact ctcaaatcgc cggtggattc cacatggaag
     1201 ccgccgcaag cgctcttgtc gatgccttct atggtgacga tccttacgtc tttgccgctt
     1261 tccacaacct tttcgcttat ggcctttcta agatcaagct ttccggcagt gtcgcccggc
     1321 atcatctcga tgcctacacc cctgttttcc gcgatcatag agttcgatat cttctcaacc
     1381 agttcctgca tttcgtcggt gagaatggat atggtgagat cgatcgtaga atactcctcc
     1441 cccatctgga accctacggt tcttgcacca agttctctat cgaagacggc cgaaagaagg
     1501 tgctgggcgg tgtgctggac ggagatatct cttctccttc tgatgtctat ctcgatatca
     1561 acggtctccc cggctttttt gtgaatgaaa ccttctatct ctatcgagca aagctctcca
     1621 tcttttttca ccgacagaat tttgtggtcg tcaacactcc ccctgtcacc taactgtcca
     1681 ccttcgccat caacgtaaac attaccgtaa caaattccga ccaaggtagt actgtctcct
     1741 aaccttattt cccttattgc ttctaacata catcgcctcc agccacttga tattatatgc
     1801 aaatatatgt aataattata cagtaaggta cgatccgctc cgtgggtgat atctcgaaag
     1861 atgaatatga ggtgatttgg ttgcaggaaa gaccgatcct ttcggttaaa aacctctcca
     1921 cacatttcaa aatggtagaa ggtgtcgtaa aagccgttca ggacgccact tttgatctat
     1981 atcgtgatga ggttctgggc attgttggag agaccggctc cggcaagagt gttacggtca
     2041 aggccatcgc cggaatgata gacaatccgg gattcatagc gggcggagag gtcctctttc
     2101 taaccgacga gttttcgaag agtggtgaaa aagagtacat agatctcgcc aagcttccca
     2161 aagagagctt ttcacgaata cgtggtaagc acatcggaat gatttttcag gatccgatga
     2221 cctctctgga ccctatgtac accataggta atcaaatgat agagaccata gtacaccaca
     2281 agggtgttac ggtagaggaa gcccgagaaa gggcagtcaa gttgctcgaa caggtgggta
     2341 ttccaaagcc ttcggagagg ataaacgatt accccttcca gctttccggg ggtcagaggc
     2401 agagagtggt aattgcaata gcgctctcgt gcgaccccga tatactgata gccgacgagc
     2461 cttctacggc cctcgacgta accgttcagg ctcagatact ggaattgatg aaggatcttc
     2521 aggaacagtt caacagcagc atgatgttca tcacgcacga tatggcggta atagccgaaa
     2581 tcgccacgaa aataaccgtg atgtacggga gttaccagat ggagatgggg caatccaaag
     2641 atatattcga acaccctatg aatccatata catatgcgtt gcttgaatgt atcccgagac
     2701 tagacgtaaa gcaggacagg cttctcccaa ttccaggcca gccgccagtg atgctcaatc
     2761 cgccggacct ttgtcccttt ctgcctcgat gttcaagggc gagcgaaaag tgttacaaag
     2821 atcttccaca gctggagcag acgggcgaca gtcatttcgt tagatgctgg aatccaatcg
     2881 caggaaaagt caagctggtc aaggaggctc aaggatgaag cttctcgaag tcaaaaattt
     2941 gaaaaagtac tttccgataa agcaaggctt cctcgtagag agggtggtcg gattcgtcaa
     3001 agccgtggat gacgtctctt tttccgttga ccggggtaag acgattggaa tagttggaga
     3061 atccggttgc gggaagacca cgataggcaa atcgattatc aggttgcatg aagtgaccga
     3121 cggagagatg ctcatagacg aggaagacac gactttctac ttcatgaagc gcaaaagggc
     3181 tctccaatat ctgaaatcta agtacttcaa ggatcaggat ttcgatggcg atggaagtgg
     3241 cctagaaacg tacaagaaga agatacacga cgtttttgtg aagtccgata gaaatccttc
     3301 aaaaacgttg aacactcttt tcgagaacct tgaagaaaag aagagactct tgagaagaaa
     3361 ggcccagata gtctttcaag acccgatgtc gtcgctcaac cccagaatga cggtcgggca
     3421 gatgctcgcc gaaccacttc tcttccacaa aatggccgaa aaaatggatg atgcgatagc
     3481 gatggtcaaa gaccttctcg tcaaggttgg tcttaaggcc taccatgtgg atagataccc
     3541 tcaccagttc agcggaggcc agcgacagag aatagccgtg gcgagggcta taagcgtgaa
     3601 tcccgacctg atagttctcg acgaaccgac ctcggctctg gatgtttcgg ttcaggcaca
     3661 gatagtgaac cttttcgaag agcttcagaa acagctgaac gcaggttatg ttttcatctc
     3721 tcacaatctt gcgctcgtga gattcatctg ccaggaagtc tctgtcatgt atctcggaag
     3781 gatcgttgaa cagggcgata gcgagtcgat attcgccgct ccactccacc catacaccaa
     3841 agccttgctc gcggcggccc cggtccccga tccgaacaaa aaacgcaatc ggaaagatct
     3901 aataggtgga caggttccaa gtccaataaa caggccatcc ggttgcttct tcaatccgag
     3961 gtgcaagtac aggatggata tatgtaccag ggaatatcca ccgatgttca aggtggatca
     4021 aaagcatttt gtagcgtgcc atctttattc cacatctcaa gaacaaggag gggaaaagta
     4081 atgaagaaat tcttagtact agtttcagta ctcttgatag caggccttgc attgtctgcg
     4141 acggagtaca aagtagaaaa cttcaacgga aaaaccggtg gaactttcta ttactgggga
     4201 ctgggcgatc ccaaatcctt taacatcgat tgggcgcagg aaaccagttc gaccgttcct
     4261 ctgggcttta tccatgctac gttgctcgaa gccgatgagg gcggtatgcc gaccgaatct
     4321 ggccttgcta aggaatggtg gttctccgac gacggactga ccttcttcgt caagataaga
     4381 gaaggtctca agtggtccga tggagctcct tttaccattg acgacgttta ctggactttt
     4441 gtggatgttt cacttaaccc cgaacagacg gccaacggca acggctctta catggactcc
     4501 aacgaccaac tccccgttgt agagatagtt gacagtacaa ccatctcctt cacctggacc
     4561 gttccgatgg tgactgcgtt gagaca
//
LOCUS       DAOOLY010000209         4535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23029_length_4535_cov_4.302679, whole genome shotgun sequence.
ACCESSION   DAOOLY010000209 DAOOLY010000000
VERSION     DAOOLY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23029_length_4535_cov_4.302679"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLD15_09555"
     CDS             complement(<1..717)
                     /locus_tag="PLD15_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64196.1"
                     /translation="MSWFRYARKRAFALPMTLILLTFCTFILLAASSQLGRNTEKMKS
                     FKTSSKMQNVATNAVEIVSHLIIQSDVEEALNGDESWDGIDEFKAFLSTKGGVEKFYW
                     ASFLSDIDSSTGGFDFTDELQSFIDDPGLGGGEYTLSAHVYKVGERYTVVTSAQIGDY
                     RTYALGLVSKLRGGILLPAVRMAGMDRVLTLMNVSGGKGNPKVTGDLIYGGAIILGGV
                     ILDMTASSTPGEIITGGVTSK"
     gene            complement(695..1069)
                     /locus_tag="PLD15_09560"
     CDS             complement(695..1069)
                     /locus_tag="PLD15_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64197.1"
                     /translation="MALAILAGFLTLGIALVISHKRMSQETTRASEIEAVASLVGSFI
                     RQDSVLKDASNAQEIAGRMNDLMIILNDTYIETSGFRDTEVSTITFHRTTDSEDLYTI
                     NYTLVDEMSEREEVYHVVVSLR"
     gene            complement(1098..1652)
                     /locus_tag="PLD15_09565"
     CDS             complement(1098..1652)
                     /locus_tag="PLD15_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64198.1"
                     /translation="MKRPKTEIRNLQPGMSLAEILAALAVSTIVLATVSMIASLSLKM
                     SGKVNASLSLDEEITKLHTSIRSIVSRQWTLLAGVPNVSDGVEGAKQAIVLSHSIPEK
                     NVLGWETALSTITYISSQKQIVHIFDTDENGGTSTSILASFVDNFSVEPIENYLKYDA
                     TFTYYSPSGVPILRRRAQGAVRFY"
     gene            1820..2776
                     /locus_tag="PLD15_09570"
     CDS             1820..2776
                     /locus_tag="PLD15_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPB64199.1"
                     /translation="MPEINSFGENLKRNMSRVIVGKGEIIERALAILLSGGHVLINDV
                     PGVGKTVFARSLAISLGLDFRRIQCTPDLLPSDVTGLNVLDIKNNEFIFKKGPVFTEV
                     LLVDEINRTTPRTQSALLEAMGERQVTIDGKTFPMSRPFFVIATQNPVEFEGTFPLPE
                     AQLDRFSVSLSMGYPGEEYEVALLKGMLLEHPLENLKTVSSRDELLKAMKRVKEIKVE
                     RSVLEYVVAIVSKTRIHRDLSLGSSPRGSIAIVDAARALAGLRGREFVIPDDIKEIAP
                     EALSHRIMLRPEARLMRRTARDIVEEILKTVPVPLQNERTKA"
     gene            2757..4040
                     /locus_tag="PLD15_09575"
     CDS             2757..4040
                     /locus_tag="PLD15_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB64200.1"
                     /translation="MRELRLESLSSRKRRLFTVSTAVFVWVILSVDTFSLMIGSLVAL
                     LWIHYFFVALSIKRLNITRTIDRERLFPGEYLTLKYSISATPLMQLELIAMPTLRGRY
                     DFASTRGERVIVRSDRTVSFQYTVSFRKRGEKDLSKLEILYRDPMKLFAIKATYSLEE
                     KIMILPRIIDPGSFPLRLRELLPGRESDFKLMEDLTDFRGIREYDREPLNRIHWKASA
                     RMGSLYSKEFGYTAVSKTFLYLDLNLSDEVFARDVWERIRVDYEETAIEVAGYLIRKI
                     IADSGRLTLVTVGEKVSYMDDTGRDWIDFFEALSAVHGCEKGPSLSECMSRDMGRYDP
                     ATTVVVLSLYLGESILPDLVKARGRANRVVVLLMPFGLRKSGQLPGKSYQMLPRAMEE
                     LKRRSSLLEEEQIIVRVVEENRSLAETFGEAGTHR"
     gene            4075..>4535
                     /locus_tag="PLD15_09580"
     CDS             4075..>4535
                     /locus_tag="PLD15_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64201.1"
                     /translation="MAVLALMQPGGLLIAIIGGSIVGLLFAYGRNRAGCSVYFLLPIT
                     LFALIILLSGTLRVDAIHTLSTVAMIAGIILAMDWRPYPALTGLLILGVAFSISPIPP
                     EIAAPVFLLLICYRLFTIPGFSRLLLVSVLVLSVLVLIVSREPVGGLMRNL"
BASE COUNT         1092 a         1120 c         1181 g         1142 t
ORIGIN      
        1 tttcgaggtt actcccccgg ttattatctc cccgggtgtg ctggaggcgg tcatatcgag
       61 gattacgccc ccgagaatta tcgctcctcc gtatatcaga tcgcccgtta ccttcggatt
      121 cccttttcca ccggagacgt tcataagtgt gagcactctg tccattccgg ccattctcac
      181 cgccggaagg agtatcccgc ccctgagttt cgaaacgagg ccgagggcgt aggttctgta
      241 atctccaatc tgcgcagaag tcacaacggt gtagcgttcc ccgactttgt agacatgggc
      301 ggaaagagtg tattcgccgc cccctaggcc gggatcgtcg atgaaggact gaagttcgtc
      361 ggtgaagtca aatccgcccg tactcgaatc tatatcgctg aggaaactgg cccagtagaa
      421 cttttcgaca ccgccttttg tggataagaa ggctttgaat tcatctatgc cgtcccagga
      481 ttcgtcaccg ttcaaggctt cttccacatc ggactggatg atgaggtgag acacaatctc
      541 aacggcatta gtggcaacat tctgcatttt cgacgatgtc ttgaaggact tcatcttttc
      601 ggtgttccgg ccgagctgac tgctggccgc aagcaagatg aatgtgcaga aggtcagcag
      661 aatcaacgtc atcggtaagg caaaggccct ctttctagcg tagcgaaacc acgacatgat
      721 aaacctcctc cctttcgctc atctcatcta caagagtgta gtttatcgta tagagatcct
      781 cactgtctgt agtgcggtga aacgttatgg ttgagacctc cgtgtctctg aagcccgaag
      841 tctctatata ggtgtcgttc aaaataatca tcagatcatt catcctcccg gcgatttctt
      901 gagcgttcga ggcgtctttc aaaacgctgt cctgccttat gaaactgccc acaagactgg
      961 ccactgcctc tatttccgag gctctggtag tttcttgcga cattctcttg tgagatatga
     1021 ccagcgcgat tcccagcgtc aaaaagcccg cgagtatcgc cagagccaca agagtctcgg
     1081 cgaaagtgaa ccctctctta atagaacctc accgccccct gagccctcct gcgcaatatt
     1141 ggtacccccg atggcgagta gtacgtgaat gttgcatcgt acttcagata gttctcgatc
     1201 ggttcaacgc tgaaattatc tacgaagctc gcaaggatgc tggtcgatgt tccgccgttt
     1261 tcatcagtgt cgaatatatg cacgatctgc ttttgcgaag agatataggt tatcgtggaa
     1321 agggccgtct cccatcccaa cacatttttt tcgggaatgc tgtggctaag aactatggcc
     1381 tgttttgcac cttcgacacc gtcgcttaca ttcggcacac cggccagcag tgtccactgt
     1441 ctggaaacta tcgaacggat cgacgtgtgc aacttcgtta tttcctcatc cagggagagt
     1501 gaggcgttca ctttaccgga catcttcaga gatagcgaag cgatcatact gacggtcgca
     1561 agaacaattg ttgaaacagc aagcgcagcc agtatttctg ccagcgacat tccaggttgt
     1621 agatttctga tttctgtttt tggccttttc atgaataaca tcacccggat acagatagaa
     1681 atagtaatac aaacatttaa gtctattgaa tgatatcatt tattgagtta caaatcaaga
     1741 aaaacataac catttcaccc tcctccaccc acacgtgata aactttttag tagttgttaa
     1801 agattacaga ggtgaagaaa tgccggaaat aaattccttc ggtgaaaatc ttaaaagaaa
     1861 catgtccaga gtgatagtgg gcaagggcga gataatagaa agggcgctgg ccattcttct
     1921 gagtggcggc catgttctca taaacgatgt tccaggtgtt ggaaaaacgg tttttgccag
     1981 aagcctggca atttccctgg ggctggattt caggagaata cagtgtacac ccgaccttct
     2041 ccccagtgat gtgacgggtc ttaacgtgct ggatatcaaa aacaacgagt tcatcttcaa
     2101 gaagggaccg gtcttcacgg aggtactact ggtagacgag ataaatagaa ccaccccgag
     2161 aacccaatcg gctcttctgg aagccatggg cgagagacag gtcacaatcg acgggaagac
     2221 cttccctatg tcaaggccat tctttgtaat agcaacccag aatcctgtag agttcgaagg
     2281 gacctttcca ctccccgaag cccagcttga caggttctcc gtttcgctct cgatgggata
     2341 tcccggtgaa gaatacgaag tagcgcttct gaaaggtatg ttgctcgagc atcctctgga
     2401 aaacctcaag accgtctcca gcagagacga gctgctgaag gcgatgaaaa gagtgaagga
     2461 aataaaggta gagagatcgg tactggagta cgtggttgcg atagtatcta aaaccagaat
     2521 tcacagggac ctatcgctcg ggtccagtcc caggggttcg atagccatcg tcgatgcggc
     2581 cagggcgctg gcgggactca ggggaagaga gttcgttatc ccggacgata taaaggagat
     2641 agctccggag gcactatccc acaggataat gctcagacct gaggcccgcc tgatgagacg
     2701 aacggccaga gacatcgtag aggaaatcct gaagaccgtt ccagttccgc ttcaaaatga
     2761 gagaactaag gcttgaaagt ttatcttcca ggaagagacg gctcttcaca gtctcgacgg
     2821 cggtcttcgt ttgggttatt ctgtcggtgg ataccttctc tttgatgatc gggtcattgg
     2881 ttgcccttct ctggattcac tactttttcg tggcattatc gatcaaaaga cttaatataa
     2941 cccgcacaat tgacagagaa agacttttcc ccggagagta tctcaccttg aaatactcga
     3001 tctccgccac tccgctgatg caactcgaac ttatcgcgat gcccacgttg aggggcaggt
     3061 acgattttgc cagcacaagg ggcgagaggg tgatcgtcag aagcgatcgc accgtgagtt
     3121 ttcaatatac ggtgagtttc agaaagcgcg gcgagaagga cctttccaaa ctcgaaatcc
     3181 tttacaggga ccccatgaag ctcttcgcca ttaaggctac ttactctctg gaagagaaga
     3241 ttatgattct tccgcgtata atcgacccgg gcagtttccc gctaaggtta cgcgaattgc
     3301 ttccgggaag ggagagcgat ttcaagttga tggaggattt gaccgatttc cgtgggatca
     3361 gggagtacga cagggaacca ctcaacagaa ttcactggaa agcctctgcg agaatgggca
     3421 gtctttattc gaaagagttc ggctacacgg cagtcagcaa gacctttctc tatctggatc
     3481 taaacctttc cgacgaagtc tttgcacgcg atgtgtggga gaggatcaga gtagattacg
     3541 aggagacggc catcgaggtc gccggctatt tgatcagaaa gattatagcc gattccggcc
     3601 gactgacgct cgttacggtg ggagagaaag tgagttacat ggacgacacc ggcagggact
     3661 ggatagattt tttcgaagcc ctttcggcgg ttcacggctg tgaaaaaggg ccgtcgcttt
     3721 cggaatgtat gtcgcgcgat atgggccgct acgatcccgc tacgacggta gtggtcctct
     3781 ctctgtacct cggggagagc atactccccg atctcgtaaa ggccagaggc cgtgccaatc
     3841 gcgtcgtggt gcttctcatg cctttcggtt tgagaaagtc cggccaactg ccgggaaagt
     3901 cttatcagat gcttcccagg gcgatggaag agttgaaaag gaggagttct ctgctcgagg
     3961 aggagcagat aatcgtgcgg gtggtggagg aaaaccggtc tctggcggag accttcgggg
     4021 aggctggtac gcatagataa gactggcagg atttcggcgt tcgttgtcgc ctctgtggcg
     4081 gtcttggcgt tgatgcagcc cggcgggctg ttgatcgcaa ttatcggtgg atcgatagtc
     4141 ggccttcttt tcgcctacgg aaggaacaga gccggctgta gtgtgtattt tctactaccg
     4201 ataactcttt ttgcactcat aatactcctg agcggaaccc tccgtgtcga tgctatacat
     4261 accttatcca cagtcgcgat gatagccggt ataattctgg ctatggattg gcgcccttat
     4321 ccggccctga cgggcctgtt gatcctgggg gtcgcctttt ctatcagccc gatcccgccg
     4381 gaaatcgcgg ctccggtctt tctgctcctt atctgctaca gattgttcac gatacccggc
     4441 ttttccaggc tgttgcttgt ctcggttctg gtgctgtcgg tgcttgtcct gatcgtctcg
     4501 agagaaccgg tcggcgggct tatgaggaat ttgga
//
LOCUS       DAOOLY010000210         4530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23066_length_4530_cov_4.879106, whole genome shotgun sequence.
ACCESSION   DAOOLY010000210 DAOOLY010000000
VERSION     DAOOLY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23066_length_4530_cov_4.879106"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLD15_09585"
     CDS             complement(<1..186)
                     /locus_tag="PLD15_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB64202.1"
                     /translation="MEALATASTDELTSVTGIGEELANSISEFFSRETVKREIDALRS
                     HVNTQEEESGVPKPLKGL"
     gene            complement(188..1783)
                     /gene="ligA"
                     /locus_tag="PLD15_09590"
     CDS             complement(188..1783)
                     /gene="ligA"
                     /locus_tag="PLD15_09590"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPB64203.1"
                     /translation="MQADEKIIERLRKLREEINHHSYRYFVLDDPVISDGEYDELMRE
                     LIDIERHYPELVTPDSPSVRVGLKPVEFFRQVVHTNRLYSLDNTYSRDEVVKFDERVR
                     EALGGESVRYACELKIDGLSISLKYEKGVLSLGATRGDGFVGEDVTTNIRAIKSIPLR
                     LRKSLDIEVRGEVYLPRDVFDELNGERSSEGLPVFANPRNAAAGTLRQLDPGQVLKRR
                     LSAFFYQVVEPMNYGLRRQEELYEFMRELGFRIEPNHETVSDTEEIVSYWKKWTTRKG
                     GLNYAIDGVVVKVDDFLQQQELGYTAKAPRWSMAFKFPAEQVSTVLTGVTFQVGRLGN
                     ITPVAELVPVQLAGTTVKRASMHNFDFIRERDIRINDTVMVEKAGEIIPQIVKSVQEK
                     RDGREIEIEMPSNCPVCGGDVGREKEGEVALKCLNPLCPAKLIRRITFFSSRDAMDIE
                     GLGERLVERLVGSGLVSGFSDLYRLTKEDMLKLGEGVGDRMAENLLSAIEKSQKNPLH
                     RLIAPGHSRDRNETGEGSGETLR"
     gene            complement(1795..2124)
                     /gene="acpS"
                     /locus_tag="PLD15_09595"
     CDS             complement(1795..2124)
                     /gene="acpS"
                     /locus_tag="PLD15_09595"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPB64204.1"
                     /translation="MKAGIDIVKISRMTERIALRILGEEEKRIYDSLGHPSRKQEFAA
                     GRFAAKEAFVKASGRKDIPFSAIEFLSNEDGSPGPSGALLEIAGSAVTVSISHEKEYA
                     IAVVILF"
     gene            complement(2135..4444)
                     /locus_tag="PLD15_09600"
     CDS             complement(2135..4444)
                     /locus_tag="PLD15_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HPB64205.1"
                     /translation="MDSSRNSVALRLLEFEAVLSEVASKTVSRYGRERVMSLEPFVED
                     PLLLYKRSSEFENIMRIQGEPPFSVFHDLYVYIDKVNLGYSLIGEELNRCATTMENTC
                     RLKEFFEPLVDSSPNVASLASQLSCERGFIAEVRKKISEEGLVKDQASPELREIRNEL
                     RGLTRNLRNRLDSIMSRYKDGLTDTLVLSRDGRYVLPLSSGKKNEYQGIIHGSSSSGA
                     TVYFEPQELISLNDTLKILQSREEEEIRRILRELTFLFQGTFERLGPSLEALGVLDSL
                     YGICRYARKRKGVFIEPSNDRTFVLREARHPLIEDDKVVPITFHMRDGINGVFITGPN
                     TGGKTVAMKTIGLSVVLMLCGLPVLADQTSSIPYFKNIYADIGDEQSIEQSLSTFSSH
                     ISRIIGIIKNTDTDSLVLLDELGAGTDPVEGAALSIAIIEKLLSTDCRLILTTHLTPI
                     KLYAMEREDVENASVEFDVNTLRPTYRLLMGIPGSSNAIEVSKRLGLPLEIIDKAQRY
                     MDSDARDLEVVIGKLHKERSTLENERRTLESTKRELESRTREYEDKLSLIKEKRYREV
                     SEEMASLEERLGRILKEIENSINLSRSERERDKVSAVKKLHDLKIQVEKHRYFAVNKN
                     EEIPEVGDTVRLLDGGTEGKVSALDGDRVIVDSGKVTIKVPVSRIRVIGKVDRTVDEI
                     PFEVQTPITSSEIDIRGNYTEDVPILVSDFIHSLKRNGLKQGYIIHGKGTGKLAESVW
                     NYLRRTKGVKNFRIGTAREGGSGVTVVEV"
BASE COUNT          913 a         1276 c         1062 g         1279 t
ORIGIN      
        1 cagcccctta agcggcttcg ggactcctga ttcttcctcc tgtgtgttca catgagagcg
       61 gagagcgtcg atttcacgct tcacggtttc tctggagaag aactcactta tagagttcgc
      121 gagctcttcg cctatgcccg taacggatgt caattcatcg gttgaggccg ttgcaagagc
      181 ctcaatatta ccgaagcgtt tcgccagatc cctcgccagt ttcgttccga tcccgggaat
      241 gcccaggggc gatcagcctg tgaagggggt ttttctggct cttttcgatt gccgacagca
      301 ggttctcggc catcctgtcg cccacaccct cacccagttt cagcatatct tctttcgtaa
      361 gcctgtagag gtcggaaaag ccgctcacaa gacctgaacc caccagtctt tccacgagtc
      421 tctctcccag tccctctatg tccatggcgt cgcgagaaga gaaaaaggtt atgcgcctga
      481 tcaactttgc cgggcagaga gggttgagac atttcaacgc cacttctccc tccttctccc
      541 gtccgacatc gccaccacac accggacagt tcgacggcat ttcgatctcg atttccctgc
      601 cgtccctctt ttcctgtacg ctctttacga tctgtggaat tatctcgccg gccttctcga
      661 ccataaccgt atcgttgatc cttatgtcgc gctcccttat gaaatcgaaa ttgtgcatgc
      721 tcgctctctt gacagtcgtt ccggccagtt gaaccggcac aagttcggcg acaggtgtta
      781 tgtttcccag tctgcctacc tgaaaagtta ctccggtgag tacggtactc acctgttcgg
      841 cagggaattt gaaggccatc gaccatctgg gggcctttgc cgtatatccc agctcctgct
      901 gctgtagaaa gtcatcgacc ttgacaacca cgccgtcgat cgcatagttg agcccgccct
      961 ttcgcgtggt ccatttcttc cagtaagaga cgatctcttc tgtgtcgctg acggtctcgt
     1021 ggttgggttc aatcctgaat cctagctcgc gcatgaattc gtagagctct tcctgtctcc
     1081 tcaacccata attcatcggc tctacaacct ggtaaaagaa ggcgctcaac ctgcgtttca
     1141 agacctgtcc cggatctagc tgtctcaaag tgccggccgc tgcgtttctg gggttggcaa
     1201 agacgggtag tccctccgaa cttctctccc cgttgagttc gtcgaaaaca tccctcggga
     1261 ggtaaacttc gcctcgcact tcgatgtcca gactcttcct gagtctcagc ggtatagatt
     1321 taatagctct tatgttggtg gttacgtcct caccgacaaa accgtctcct ctggtcgctc
     1381 caagcgagag gacccccttt tcgtacttca gggaaatgga cagtccatcg atcttcagct
     1441 cgcaggcata acggacggat tcaccgccga gggcttctct gactctctcg tcgaacttca
     1501 ccacttcatc tctggaatag gtgttgtcga gtgaatacaa tctgttggta tgtaccacct
     1561 gacgaaagaa ttcgaccggc ttcaaaccga ccctgacgct tggagaatcc ggggtgacca
     1621 gttccgggta atgtctttct atatcgatca gttctctcat gagttcgtcg tattcgccgt
     1681 cggatatgac gggatcgtcc agaacaaagt atctgtaaga atgatgattg atctcttctc
     1741 tcagctttct caatctttct attatcttct cgtcggcttg caagatcttc cctcctaaaa
     1801 aaggatgacc acggcgatcg cgtattcttt ttcgtgagat atactcacag tgacggccga
     1861 accggcgatc tcaagaagag ccccggacgg accgggagaa ccgtcttcgt ttgagaggaa
     1921 ttcgatggca gagaagggta tatcctttct gcccgatgcc ttcacgaagg cctctttggc
     1981 cgcaaaacgc cccgccgcga actcctgttt tctcgatgga tgaccgaggg aatcgtagat
     2041 ccgcttctct tcttctccaa gtattctgag ggctatcctc tctgtcattc tggatatttt
     2101 tactatatct atccccgctt tcataggccg tccttcagac ctccaccacg gtgacgccgc
     2161 ttccgccctc tctggccgtt ccgattctga agtttttcac gcccttggtc ctgcgcaggt
     2221 agttccatac gctctcggcc agttttcccg ttccctttcc atgtataatg tatccctgct
     2281 tcaaaccgtt tctcttcaga gaatgaatga aatccgacac caggatcggg acatcttccg
     2341 tgtagtttcc gcggatgtct atctccgaac tggtgatggg agtctgaact tcgaagggga
     2401 tctcatcgac ggttctatcc actttcccga tcacccttat cctggagacc ggcactttga
     2461 tggttacctt gcccgaatct acgataaccc tgtcgccgtc gagcgccgag accttccctt
     2521 ctgtgccacc gtctaacagt ctgacggtgt ccccgacctc ggggatttct tcgttcttgt
     2581 tgactgcgaa gtacctgtgc ttttcaacct gaatcttaag atcgtgcagt ttcttgaccg
     2641 ccgaaacctt gtccctctct ctttccgatc tggaaaggtt gatcgagttt tctatctctt
     2701 tgagtattcg gcccagtctt tcttccagag atgccatctc ttcgctgact tctctgtatc
     2761 tcttctcttt gatgagagaa agcttatcct cgtactctct tgttctgctt tccagttctc
     2821 tctttgtgct ctccagggtt ctcctctcgt tctcgagagt cgatctctct ttgtggagtt
     2881 tgcctatcac gacttcgagg tcgcgagcat cgctgtccat gtatctctgt gccttgtcta
     2941 tgatctccag gggaagaccg agtctcttcg agacttctat tgcgttagag gagccgggaa
     3001 ttcccattaa aagtctatag gtgggtctga gagtgtttac atcgaactcc acgctggcgt
     3061 tttccacgtc ttccctttcc atagcgtaca gctttatggg agtcaggtgc gtcgttagta
     3121 tcaaccggca gtcggtggat aagagtttct ctattatagc tatcgaaagt gccgcccctt
     3181 caacaggatc ggtaccggcc cccagttcat ccagcaggac cagagagtcg gtgtccgtgt
     3241 tctttattat gcctatgatc ctcgaaatgt gcgaagagaa ggtactgagg ctctgctcta
     3301 tggattgttc gtctcctata tcggcgtata tgtttttgaa gtagggtata gaggaagtct
     3361 gatcggccag aaccggcaat ccgcaaagca taagcactac ggaaagtccg atcgtcttca
     3421 tcgcgaccgt ctttcctccg gtgttcgggc cggttatgaa gacgccgttt atcccgtctc
     3481 tcatgtgaaa ggttatggga acgactttat cgtcttctat gagcggatgg cgcgcctccc
     3541 tgagaacgaa ggttctgtcg ttcgacggtt ctatgaagac gcctttcctc tttctggcgt
     3601 atctacatat tccgtagaga ctgtccagca ctcccagcgc ttcgagagag ggcccgagcc
     3661 tttcgaaagt gccctggaag agaaaagtta gctccctgag tattcttcta atctcctcct
     3721 cttcccgcga ttgaaggatc tttagggtgt cgttcagcga gatcagctcc tgtggttcga
     3781 aatacacggt cgctccgcta ctcgaagagc cgtgtattat accctggtac tcgttctttt
     3841 tacccgagga gagggggagc acatacctcc catccctgct cagaacgagc gtgtcggtca
     3901 ggccgtcttt gtaacgcgac atgatcgagt ccagtctgtt tctaaggttt ctggtcaatc
     3961 cccggagttc gtttcttatc tctctcagtt ccggggaggc ctgatccttc accagtccct
     4021 cttcagagat tttctttctc acctctgcaa tgaaacctct ctcgcacgac aactgcgatg
     4081 ccaacgacgc cacgttagga ctggaatcga ccaggggttc gaagaactcc tttagtctgc
     4141 aggtgttttc catggtagtt gcgcacctgt tcagctcctc gcctatgagc gaatagccga
     4201 ggttgacttt gtcgatgtaa acatatagat cgtggaaaac cgaaaagggc ggttcgccct
     4261 gaattcgcat gatattctcg aactcgctgg agcgtttgta aagaagaagg ggatcctcta
     4321 caaatggttc tagcgacatc accctttccc tgccgtacct ggaaaccgtt ttagatgcga
     4381 cctccgaaag tacggcttcg aactccagaa ggcgaagagc cacactattg cgtgatgaat
     4441 ccatctggca ggaccacctt tctgactatt cggggcatca atttctggcg gttgcgcctg
     4501 tcgtatatcg atatgatgcc gtaaaccact
//
LOCUS       DAOOLY010000211         4517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23155_length_4517_cov_4.147019, whole genome shotgun sequence.
ACCESSION   DAOOLY010000211 DAOOLY010000000
VERSION     DAOOLY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23155_length_4517_cov_4.147019"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..620
                     /locus_tag="PLD15_09605"
     CDS             12..620
                     /locus_tag="PLD15_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB64206.1"
                     /translation="MKEGFDHYNTDKKQPPRTIKTVKFDLKNGHQYIFKSPEGVFAYG
                     KVDRASTILVENCVVSAQDRVLDIGSGFGMVGITLKREYPDISLFMSDVNTRAVTFSK
                     INARDNNVQADVRKGDLYEPWKDMEFDVILSNPPMAAGKSVWEKIVLEAPTYLALGGS
                     LQVVAFHNKGGSRIEGIMRQVFGNCTALVKSGGIRVYLSRKA"
     gene            667..1311
                     /locus_tag="PLD15_09610"
     CDS             667..1311
                     /locus_tag="PLD15_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64207.1"
                     /translation="MISNVSLVLHQGESLAIIGLNGSGKTSLLRSIAGILEEVSGEIR
                     VDGRIGMVFQDPGLSLFGTTVEENLLFSLSGCEDNGEIISSKIETSLQRYSIVELRKR
                     DILSLSGGERQRVALAAATVISPDLLLIDEPFSMLDRLEIKNMLDILHTLTERGTSIV
                     LSTNRFSDLSVFDRFLVLGNGSMVFYGNWELLKKNREIFTLAGMPVPFELELSC"
     gene            1308..2090
                     /locus_tag="PLD15_09615"
     CDS             1308..2090
                     /locus_tag="PLD15_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64208.1"
                     /translation="MKIKNLSFAYPSRRSELVEVISDISFEANEGSLVLLTGSSGSGK
                     TTLLKLLCGLLKAQSGEVTLDDESVERRATMVFQSPEESLFNATVREEIIFAFKESCY
                     PDVEDRYRDIMSSCGLDHEKYSSRSPLSLSGGEQRRVGLAAMLGLSKKLLLLDEPAAG
                     LDEPGIENVRKIVVSERAAGRIIFIATHNLMDFLDLATHVATLDRGRLKYFVTIDGYI
                     LEKVRAEDLELKGKLMVDYYRNFGRFPGDEELLSYWRGEMNC"
     gene            2084..2761
                     /locus_tag="PLD15_09620"
     CDS             2084..2761
                     /locus_tag="PLD15_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPB64209.1"
                     /translation="MLNISVIVNTLAVIAGSLLGIFGGKLVKESYRKVFFNVIGLLTM
                     ALGIEMFLDTSSVLVVLGSLIVGGAIGQALDIEEKIGKLTGKKSDDENFVKGFITASV
                     LFVAGPMTVIGSLKAGLSGENELIFIKSLMDGISSIMLAAAMGRGVLLSAGAVYVVQG
                     SLVSLAGLLSFLQGPIYLGDFSGVGGLMLLGLGLRLLEIKEIRVGNFLPALIVSPLLT
                     WVSTLFT"
     gene            complement(2754..3605)
                     /locus_tag="PLD15_09625"
     CDS             complement(2754..3605)
                     /locus_tag="PLD15_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPB64210.1"
                     /translation="MTYFRECEPAWCPGCGNFPLRKNLIETLDELKIPKELFVMVSGI
                     GQAAKMPHYLEMNFFNGLHGRSLPVAFAIKAVNPRLTVVAESGDGDMYGEGGNHFIHG
                     IRRNIDITVLIHDNQIYGLTKGQGSPTTQKGQVTSSQLQGVINEPLNPLALAITMDAT
                     FVGRTFIGDSEHFKRIVTAAIKHRGFSVVDIMQPCVTFNKVNTFKYYKNRLYDVKEEN
                     PSYDPGNKARAFEKSLEWGERIPMGIIYTRDKPSYSELHAVLKEGVVPVKDSKEFNLE
                     RELEVFR"
     gene            complement(3602..>4517)
                     /locus_tag="PLD15_09630"
     CDS             complement(3602..>4517)
                     /locus_tag="PLD15_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPB64211.1"
                     /translation="VGASFAGARSLVTTSGGGFALMQEGVSLAAMTETPLVIVDAQRP
                     APATGLPTRTAQEDLHFVIHAGHGEFPKYVVAPRTARDAFEITQKAFYIADKYQIPVI
                     ILLSQCLTDSSATIEKPTYIEEYLERAIVDGEENYKRYILTSNGVSPRAIPGGKALVK
                     VDSDEHDEYGHITEDLKIRKEMVEKRMKKLDNLVRELSEPLKFNLDAPLVLVGWGDSW
                     GAIDEFARSRKGVGYIHYNEVFPLDASVANLLRGKKVVTVEGNFTGQFATLLKSITGL
                     EVKHMGRYDGRPINAAWIAKEIEREGLS"
BASE COUNT         1030 a         1123 c         1184 g         1180 t
ORIGIN      
        1 ggaagactgg gatgaaagaa ggctttgacc actataacac tgataaaaaa caaccgccga
       61 gaacgatcaa gacagtgaag ttcgatctca aaaacggtca tcagtacatc ttcaagagcc
      121 ccgaaggcgt gtttgcctat ggcaaagtcg atcgggcatc gacgatactg gtcgaaaact
      181 gcgttgtttc cgcccaggac cgcgtgctgg atataggtag cggtttcggg atggtcggaa
      241 ttaccttgaa gcgcgaatac ccggatatat ctctcttcat gagcgacgta aacaccagag
      301 cggtcacctt cagcaagatc aacgccagag ataacaacgt tcaggccgat gtgagaaagg
      361 gtgatcttta cgaaccatgg aaagacatgg aattcgatgt aatcctttcc aatccaccca
      421 tggcagccgg aaaatcggtt tgggagaaga tcgttctgga ggcaccgaca tatctcgcgc
      481 tgggtggaag tctgcaggta gtcgcctttc ataacaaagg cggatcgcgc atcgagggga
      541 taatgaggca agtcttcggc aactgtaccg ctctggtgaa gtccggtggt atccgtgtct
      601 atctctccag aaaagcgtga aattctctct ctgagaggag tatccatctc cttccgtggt
      661 aatcgggtga tttcaaacgt atctcttgtt cttcatcagg gtgagtcact cgctatcata
      721 ggcctcaacg gttcggggaa gacatctctt ctcagatcca tagccgggat attagaggaa
      781 gtctcgggcg agatccgcgt cgatggcaga atcgggatgg ttttccagga cccgggccta
      841 tcgctcttcg gtacaaccgt agaagaaaac cttttgttct cgctcagcgg ttgcgaagat
      901 aacggagaaa taatctcttc gaagatcgaa acctctcttc aaagatactc tatagtggag
      961 ttgagaaaga gggatatact ttcgctctcc ggtggagagc gccagcgtgt tgctctggcg
     1021 gccgctaccg ttatctcacc ggacctcttg cttatcgacg agcctttctc gatgctggac
     1081 aggctcgaga taaagaatat gctcgatatc ctgcacactt tgacggagag aggtacttca
     1141 atagtgctga gtaccaacag gttctccgat ctctcggtct ttgaccgctt tctcgtactt
     1201 ggaaacggtt cgatggtctt ctacggaaac tgggagctgc ttaaaaagaa tcgtgagatc
     1261 ttcacgctgg ccggcatgcc ggtgccattc gaactggagc tctcatgttg aaaatcaaaa
     1321 acctttcctt tgcctatccc tcgagaaggt ccgagctggt agaggtcata tcggatatct
     1381 ccttcgaagc aaatgaaggt tcgcttgttc ttcttacagg atcgagcggc tcgggaaaaa
     1441 cgactctcct gaagcttctt tgcggtcttc tgaaagcgca atcaggtgaa gtaaccctcg
     1501 acgacgagag tgtggaaaga cgtgcaacga tggttttcca gtctcccgaa gagtcactct
     1561 tcaatgccac cgtcagggag gagattattt tcgccttcaa agagagctgc tatcctgacg
     1621 ttgaagaccg gtacagagat atcatgagca gttgcggact agatcacgag aagtattcat
     1681 cgcgttctcc gctcagcctt tccggtggtg aacaacggcg agtaggactt gcggcgatgc
     1741 tcggcctttc gaagaagctc ctccttctcg atgaaccggc cgccgggcta gacgagcctg
     1801 gaattgaaaa cgtacgaaaa atcgtcgtgt ccgagcgtgc cgccggcaga ataatcttta
     1861 tcgcaacaca caatttaatg gattttctcg atcttgcaac ccacgttgcg actctagaca
     1921 ggggaaggtt gaaatatttc gttacaatag acgggtatat tcttgaaaag gtccgtgccg
     1981 aagatctaga actcaaaggg aagttgatgg tcgattacta tcgcaatttt ggtcgctttc
     2041 ccggtgacga ggagctttta tcctattgga ggggtgaaat gaattgctaa acatctctgt
     2101 gatcgtcaac actctggccg ttatcgctgg ttcgctcctg ggaatcttcg gtggaaagct
     2161 tgtcaaagaa agctacagga aggttttctt caacgtgatc gggttgctga ccatggcact
     2221 gggaatagag atgtttctcg ataccagcag cgtattggtg gtcctcggaa gcctcatagt
     2281 cggaggagcc atcggccagg ctctggatat agaggaaaag attggaaaac tcacgggaaa
     2341 gaaaagtgac gatgagaatt tcgtgaaagg cttcataact gcttccgtgc tcttcgttgc
     2401 cggtcccatg actgtgatag gttctttgaa agccggtttg agcggtgaaa atgaactcat
     2461 cttcataaag tcgttgatgg acggtatctc ttcgataatg ctcgcggctg ctatgggcag
     2521 aggcgtacta ctctcggccg gagcggtata tgttgtacag ggttctctgg tttccctggc
     2581 cggtcttctc tcctttctcc agggtccgat ctatctgggc gatttttcgg gagtcggagg
     2641 cctgatgttg ctggggctgg ggttgaggtt gctcgaaata aaagaaatca gggtcggcaa
     2701 tttcctcccg gccctgatag tatctcctct acttacatgg gtttcgacgc ttttcacctg
     2761 aagacttcga gctctctctc caggttgaac tccttcgaat ctttcactgg aaccacgccc
     2821 tccttcagaa cggcgtgcaa ttcactgtaa gagggtttgt ctctggtgta tatgatgccc
     2881 atcggaatgc gctctcccca ttccagagac ttttcgaaag ctctcgcctt gttgccggga
     2941 tcgtacgatg ggttctcttc tttgacgtcg tacagacggt ttttgtaata tttgaaagtg
     3001 ttcaccttgt tgaaagtcac gcagggttgc attatatcga ccaccgaaaa gcccctgtgc
     3061 ttgatcgcgg ctgtgactat tctcttgaaa tgctcgctgt cgccaataaa ggttctgccg
     3121 acgaacgtcg catccattgt gatcgcaagc gccagggggt tgagcggttc gtttatcaca
     3181 ccctgtaact gggaactcgt gacctgaccc ttctgagtcg taggagagcc ctgccccttg
     3241 gtgagaccgt agatctgatt gtcgtgaatg aggacggtga tgtctatgtt tctccttatg
     3301 ccgtgaatga agtggtttcc accctcgccg tacatatctc cgtcgcccga ttcggcaacg
     3361 accgtgagtc gcggattgac ggcctttatc gcgaaggcca ccggtaagct cctgccatga
     3421 agaccgttga agaagttcat ttcgagatag tgtggcatct ttgcggcctg gccgattccc
     3481 gaaaccataa cgaagagctc tttagggatt ttcaattcat ccagcgtttc gatcagattc
     3541 tttctgaggg gaaagttccc gcaaccgggg caccatgcag gttcacactc tctgaaatat
     3601 gtcatgacag cccctccctt tctatctctt tcgctatcca ggcggcgttt atcggtctgc
     3661 cgtcgtacct tcccatgtgt ttcacctcta gtccggtgat cgattttaga agcgtggcga
     3721 actggccggt gaagttccct tccaccgtaa cgaccttttt accgcgcagg agattcgcca
     3781 cggaggcatc gaggggaaac acttcgttgt aatgtatata acccacgccc tttctgctcc
     3841 tggcgaactc gtcaatcgca ccccagctgt cgccccagcc gaccagaacg agcggggcat
     3901 cgaggttgaa cttcagaggt tcggagagtt cacgaacaag gttatctagc tttttcatcc
     3961 gtttctcaac catctctttg cgtatcttca gatcttcggt tatatgaccg tattcatcgt
     4021 gttcgtcgga atcgaccttc accagcgctt taccgccggg tatggctctt ggagatacgc
     4081 cgtttgaagt gaggatgtat cttttgtaat tctcctcgcc atcgacaatc gctctttcca
     4141 ggtattcttc tatgtacgtc ggtttctcta ttgtcgcgga tgaatccgtc agacattgac
     4201 tcagaagtat gatgacgggt atctggtact tatcggctat gtagaaggcc ttctgtgtga
     4261 tctcgaaggc gtcccgggca gttctcggag cgaccacata tttcgggaat tcgccgtggc
     4321 ccgcgtgaat cacgaagtgg agatcttcct gcgcggttct cgtgggcagt ccggtcgccg
     4381 gagccgggcg ctgagcatct actatcacaa gcggggtttc cgtcatcgca gccagcgaaa
     4441 caccttcctg catcagggcg aacccccctc cggaagttgt tacgagagat ctggcaccgg
     4501 cgaaggaagc gcccacg
//
LOCUS       DAOOLY010000212         4500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23263_length_4500_cov_4.757480, whole genome shotgun sequence.
ACCESSION   DAOOLY010000212 DAOOLY010000000
VERSION     DAOOLY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23263_length_4500_cov_4.757480"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1412)
                     /locus_tag="PLD15_09635"
     CDS             complement(42..1412)
                     /locus_tag="PLD15_09635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF041004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HPB64212.1"
                     /translation="MFGRDFVFGVSLSGFQFEMGRACPDSVDRGSDWYLWTHDKLNIE
                     NGIVSGDFPEDGPNYWNNYGEYHRLAAYSGMKMVRIGLEWSRIFPSPTFDITDVELEG
                     ICDRSTLEHYRKIIKDIKERGMKVVVNLNHFTLPLWLHEPVKVNRLSDFTAGGWVDPR
                     SVEQFRKFAALCGRELGDLVDMWSTQNEPNVVAMLGYRNRASGFPPSIIRPELVEVAR
                     NNLIEAHLVAYDELKRTSDLPVGLIYAFSWIDGEESAVNLAMKKTQFEFVDRISNKLD
                     FLGCNYYSRMVVESDPSSDNGYRTLRGYGQNCEPNSLSGYGRPTGDFGWEIYPEGLYN
                     ILKMICRRYDVPVYVMENGIADMADHYRPYYLISHLKALEKLLEEGYNIKGYLHWSLT
                     DNYEWSSGLGKQFGLIGVDFSDGKLTPRPSFYLFKEIIGQGTVKNFEKMLATPYRIFD
                     TDLLIE"
     gene            complement(1417..2319)
                     /locus_tag="PLD15_09640"
     CDS             complement(1417..2319)
                     /locus_tag="PLD15_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64213.1"
                     /translation="MLELINLTKVFKTGTFSSGRLKAVNNVSFEIERGEVTSLIGESG
                     SGKSTIGKMILRLLTITAGEIKLDERSISTIKGAELRQYYRKVQGVFQDPFSSYNPIF
                     KVDRVFENLRDVFFRELRHSEWKDKVNSALKLVGLDSEQVLGKYPHQLSGGQLQRILI
                     SRALLLDVSFLVADELISMLDASTRIDVLNLLADLKKGGLSILFITHDLSLGYYLSEK
                     AVILYRGHVVEMGSTDRVFLNPVHHYTRRLISSVPRLDRKWKDIDDLQKNEEHSLKCF
                     DPIEDGELEMVEVEKDHYVAVKRR"
     gene            complement(2320..3270)
                     /locus_tag="PLD15_09645"
     CDS             complement(2320..3270)
                     /locus_tag="PLD15_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64214.1"
                     /translation="MSLNVANLRVYYQTLKGDVKALDGVTFNLRDGEIAGIAGESGCG
                     KSTLSNSLITLRLPMKFVEGEVSLDGNELPVHDHKKMVDFRYKKISIIPQFAMNALNP
                     TVKLRRLISDLLNCHGVGYDEILPELNRRLDMVGLDRTVLDMYPVELSGGMKQRAVMV
                     ISTLLNPSLVIADEITSALDVSTQRAVAEMIVEFRDKKLVKSVIFVTHDISILYQIAD
                     SIIIMYAGKLVEHAGTEIIMKKPIHPYTEMLISSLPEVGVYHNDKRLKGIPGKPPQLL
                     SPPPGCRFKERCPQAFKKCEEEPPMTEIEPDHWVACWGVR"
     gene            complement(3273..4121)
                     /locus_tag="PLD15_09650"
     CDS             complement(3273..4121)
                     /locus_tag="PLD15_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64215.1"
                     /translation="MKTKNEFLYFALKNGRLKVGLFILLFFVLLAIFGPLFAKYGPLD
                     YAGPGYRPPSNEFWLGTTTFGQDVFSQTVHGLRATFVVGLVAGGLSTLLGILVGFIAG
                     YRGGMVDELLNMLTNIVTVIPTLALLLIVAAYLPYRGILVESIFIGLTAWPWAARAIR
                     AQTFTLRTRDFVDLARITGMKPSKIIFKEIAPNMMSYLFMTFILQFGGAILAAVTLDF
                     IGLGPTQGISLGLMMQNAVLWSAIQLGMWWWLIPPGVAITAIVGALYFMNTGLDEVFN
                     PKLREM"
     gene            complement(4118..>4500)
                     /locus_tag="PLD15_09655"
     CDS             complement(4118..>4500)
                     /locus_tag="PLD15_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64216.1"
                     /translation="GGWAIGMRNMIIYELEAEYSRYLESLGASRKLIRRYAYKNAVLP
                     QITGLALQLGVIVAGALATEVVFSYPGIGHLLKEAILNQDYFLIQGCFLFIIIGVLLA
                     NIILDFAYILIDPRIRHSAGGETI"
BASE COUNT         1115 a         1154 c         1016 g         1215 t
ORIGIN      
        1 cggccgcgac gtcttttatc gtaaccattg aaactttccc cctattctat aagcagatcc
       61 gtatcgaaaa ttctgtaagg tgttgcaagc atcttttcaa agtttttcac cgtaccctgt
      121 ccgataatct ctttgaaaag atagaacgaa ggtcttggtg tcagtttccc gtctgagaaa
      181 tccactccga tcaacccgaa ttgctttccc agaccggatg accattcgta attatcggtg
      241 agcgaccagt ggagatatcc ttttatgttg taaccttctt ccagcagttt ctccaaagcc
      301 ttgagatggc ttatcaggta gtaaggtctg tagtgatcgg ccatgtcggc gataccgttt
      361 tccattacgt acacaggtac gtcgtatctt ctacaaatca tcttcagtat attgtacagc
      421 ccttcgggat agatctccca gccgaagtct cccgtcggtc ttccatatcc ggaaagtgag
      481 ttgggttcac aattctgtcc atagccacgg agagttctat atccattgtc gctagagggg
      541 tcactctcta caaccatccg tgaataatag ttacaaccca gaaaatccag tttatttgag
      601 attcgatcga caaattcgaa ctgagtcttt ttcattgcca gatttacggc cgactcctct
      661 ccgtcgatcc agctgaaggc gtagataagt cccaccggca aatccgaagt cctcttcagt
      721 tcgtcgtacg caaccaggtg agcctcgatc aagttgtttc gggcaacttc gaccagttcc
      781 ggtcttatga tcgaaggagg aaagcccgag gccctgtttc tatagccgag catggccacg
      841 acgttgggtt cgttttgagt tgaccacatg tccactagat cgcctagctc tctgccacaa
      901 agcgcagcga actttcgaaa ctgctcgacc gatctgggat cgacccagcc tccagccgtg
      961 aaatcggata atctgttgac cttaactggt tcatgaagcc agaggggtag tgtgaagtgg
     1021 ttaaggttga ccaccacttt catcccccgt tctttgatat cttttatgat tttccggtag
     1081 tgttctagag tgctacggtc acagatacct tcaagttcaa catcggttat atcaaaagtg
     1141 ggggacggga aaatcctcga ccattccaga cctattctca ccattttcat tcctgagtaa
     1201 gcggctagcc tgtggtactc cccgtaatta ttccagtaat tcgggccgtc ttcgggaaaa
     1261 tctccgctca cgataccgtt ttcgatgttc agtttatcgt gagtccatag ataccagtcc
     1321 gaacctcgat cgacactatc tggacaggct ctccccatct cgaattgaaa acctgaaaga
     1381 gaaacgccga atacgaaatc tctgccaaac atatgttcat ctccttttca cggctacata
     1441 gtggtctttt tcaacttcca ccatttcgag ttctccgtct tcgatgggat cgaaacactt
     1501 cagactatgt tcctcatttt tctggagatc gtcgatgtcc ttccattttc tgtccagcct
     1561 cggcactgag gagatgagtc tgcgagtata atggtgaacg ggattaagga agaccctgtc
     1621 tgtacttccc atttccacaa cgtggcctct gtataaaatg accgcttttt cgctcagata
     1681 gtatccaaga gacagatcgt gagtaataaa gagtatcgaa agtccccctt tcttcagatc
     1741 tgcgagaaga ttaaggacgt cgatccttgt cgaggcgtcc agcatactga taagttcgtc
     1801 tgctacaagg aaactcacat ccagtaatag agccctggat atgaggattc tctgtagctg
     1861 ccctccactg agctgatggg gatactttcc aagaacctgc tcagagtcca gtccaaccag
     1921 cttgagggcc gaattcactt tgtctttcca ctcgctgtgg cgcaattctc tgaaaaagac
     1981 atcgcgcaag ttctcaaaga ctctatccac cttgaaaatg gggttgtatg aactgaaagg
     2041 atcttgaaag acgccctgga cttttctgta gtactgtctc aattccgccc cttttattgt
     2101 gcttatgctc cgttcgtcta gctttatctc gcctgcggtt atagtgagca gcctcaatat
     2161 catctttcct atagtgctct tcccgctacc gctctctcca atcagagagg tcacttcgcc
     2221 tctttcaatt tcgaacgaaa cgttgttcac agccttgagc cgcccggagg aaaacgtccc
     2281 cgtcttgaaa actttggtaa ggtttattaa ttcaagcatc tacctcaccc cccagcacgc
     2341 cacccagtgg tccggttcga tttcggtcat ggggggctct tcttcacatt ttttgaaagc
     2401 ctgcgggcag cgttccttga atctacaacc cggcggggga gagaggagtt gaggtggttt
     2461 tcccggtatt cccttcagtc ttttatcgtt gtggtataca cctacctcgg gtagagaaga
     2521 tattagcatt tcggtgtatg gatgaatagg cttcttcatt ataatttccg tgccggcgtg
     2581 ttctacaagc ttgccggcat acattattat tatcgaatcg gctatctggt agagaattga
     2641 gatatcgtgg gtgacgaata tcacgctttt tacaagcttt ttgtccctga attcgacgat
     2701 catctccgcc acagcccgct gggtcgagac atcgagggcc gaggtgatct catcggcgat
     2761 cacaagcgac gggttcagaa gtgtggaaat gaccatcacc gctctctgtt tcatcccccc
     2821 ggaaagctcc acagggtaca tatcgagtac agtccggtct aggccgacca tatcgagcct
     2881 cctgtttaat tcaggaagaa tttcatcata ccccactccg tgacagttta gaagatccga
     2941 gataagtctg cgcagtttca ctgtggggtt gagagcgttc atggcaaact ggggtattat
     3001 tgagatcttt ttatatctga agtcaaccat tttcttatga tcgtgaaccg ggagctcgtt
     3061 cccatccagg gagacctccc cttcgacgaa tttcatggga aggcgtagag ttatcaacga
     3121 attgctgagt gtcgattttc cgcaaccaga ttccccggca attcctgcta tctctccgtc
     3181 tcgaaggtta aaagtaacac catcgagagc cttcacgtct cctttgagcg tttggtaata
     3241 taccctcaga ttggcaacgt tgagagacat aatcacatct ccctcaattt tggattgaag
     3301 acttcgtcaa ggcctgtgtt cataaagtac aacgccccaa ctatggccgt tatggcgact
     3361 cccggcggta tcaaccacca ccacattccg agttgaatcg cactccagag aacggcgttc
     3421 tgcatcataa gccccagcga aattccctga gtgggaccca acccgatgaa atctagggta
     3481 acggctgcaa gtatcgctcc accgaactgg agtataaagg tcatgaaaag gtacgacatc
     3541 atgttggggg ctatctcttt gaaaattatc ttcgaaggtt tcatacctgt gatcctggca
     3601 agatctacga aatctctcgt tctcaaggtg aaagtctgtg cccttatcgc cctcgccgcc
     3661 cacggccagg cggtcagacc tatgaagata ctctcgacga gtatccccct gtagggcaag
     3721 taagccgcta ctatcaataa aagagccagt gtaggtatca cggtgactat gttggtcaac
     3781 atgttaagca gttcatcaac catacctcct cggtaaccgg cgatgaagcc gacaaggatt
     3841 ccgagcaagg ttgagagacc accggcgacc agtccgacta cgaaagtggc cctgagaccg
     3901 tgaaccgtct gggagaaaac atcctggccg aaggtcgtgg tccctagcca gaattcgttc
     3961 gatggaggac gataacccgg tccggcgtag tcaaggggcc catacttggc gaatagtgga
     4021 ccaaagatcg ccaacaacac gaagaatagg agtatgaaga ggccgacttt caaccgtccg
     4081 tttttcagtg cgaaatacag aaattcgttc ttcgtcttca tatcgtctct ccccccgccg
     4141 aatgtctgat tctcggatcg atcagaatgt atgcaaaatc cagtattata ttggccagca
     4201 agactccgat gattatgaag agaaaacatc cctgaattag aaagtaatcc tgattaagta
     4261 tagcttcctt gagtagatgt ccgatgccgg gataggagaa aaccacctcg gtggcgagcg
     4321 cgccggcaac tatgacaccc agctgaaggg ccagcccagt tatttgagga agtacggcat
     4381 ttttgtaggc gtaacgcctt atgagttttc gcgaggctcc gagtgattcg agatatctgg
     4441 aatactctgc ttccaattcg taaattatca tattgcgcat tccaatagcc caacctccga
//
LOCUS       DAOOLY010000213         4498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23279_length_4498_cov_4.137070, whole genome shotgun sequence.
ACCESSION   DAOOLY010000213 DAOOLY010000000
VERSION     DAOOLY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23279_length_4498_cov_4.137070"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..475)
                     /locus_tag="PLD15_09660"
     CDS             complement(86..475)
                     /locus_tag="PLD15_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPB64217.1"
                     /translation="MEGHITCVICPVGCKVSVRKEGVQYTIEGNRCARGEEYARNELM
                     MPKRILTTSIGVSNGTLPLVSVKTPRPIDRARIKEIMKEIKNLSIAAPVAVGDTVVED
                     IDGEGTPLLATRAVSCNGGREKTTLPT"
     gene            complement(475..1713)
                     /locus_tag="PLD15_09665"
     CDS             complement(475..1713)
                     /locus_tag="PLD15_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB64218.1"
                     /translation="MLKREKTDVLVIGGGAAGMACALSAAKEGVYVTLLERESSVGGV
                     LNQCIHNGFGLQFYHQELTGPEFASRLIDDMNMENVKIISQSYVRDIDVRKRVAQVLS
                     PDGAYEIQAGALVISTGARERPFGSLLIPGDRPAGVMPAGVAQRYVNLENRIPARKAM
                     ILGSGDIGLIMARRLTLEGVEVVSVLERMSYPGGLTRNIVQCLEDFDIPLHLSTTVTG
                     ISGDGRLRSVEISKVDENFTPVPGTSRTIDVDALILSAGLLPQVEDFSDDLEVDPMNR
                     GFLVSNTCESSTEGVFAAGNNVAIFDLVDYVAAEGWIAGRHAALFSRGVNTSGDRVPV
                     FRGKNISVLVPGIVDMEEHLRLYIRLRKPMEKGVVVLKELKIEKKFSYGVPSEMIQLV
                     VNRDKLQPLMNYGKLTVEVQ"
     gene            complement(1707..3158)
                     /locus_tag="PLD15_09670"
     CDS             complement(1707..3158)
                     /locus_tag="PLD15_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB64219.1"
                     /translation="MRSVVIGGGVIGTLIARQLSKFEGEVILIEKREDLGQGITKANS
                     AIIHGGYDDPPASLRGRLCARGNELYDRLARELKVPVNRIGSIVVARNEEELKKLEEL
                     LVNGQANGVRDLRIVGREELHELEPAISSVFNYALYCGSAGITEPWMVAIAASINAAR
                     NGAEIITGDGVVGGRVEEGSIKSLRLASGREIKNIDIVINATGLFYEKVARLFDVEVP
                     PVKLRRGEYILLDKRASDLVRRIVFPLPTASGKGRLVLPTIDGGVLLGPTSEELQDFT
                     PQELKTTAEGLYSVRQSGKELIPGIDSPQWYVKSFAGIRPESSGKDFYIKAACEIDNF
                     ITVGAIRSPGLTAAPAIAEYVVLELLPSIGVSLEEKRNFDPFIEEQTRIKELPFEAIS
                     SLIESDPLYGKIICQCNEVSEAEIVQSIRDGARTIDGVKFRTRAGFGRCQGGFCSWKI
                     ASILSRELGKDLSEIRQNVPESWITDGKVRERC"
     gene            3337..3876
                     /locus_tag="PLD15_09675"
     CDS             3337..3876
                     /locus_tag="PLD15_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HPB64220.1"
                     /translation="MLKGIIAALWDRKTRPEEVDPRTVFFLNGGLFEIQDRINEFKAA
                     GKSVFVDIDFISGLSGDEDSVLFLKKCGIDGIITAKIRISKQAISLGMNSLLRFFALD
                     SRAVDKGIQQILSNGVKNIEILPGLVSTKVAPRIRAMVPDVNLVAAGLIDTSEELDLL
                     KKYVDGVSTSSTVLWRYKW"
     gene            complement(3877..>4498)
                     /locus_tag="PLD15_09680"
     CDS             complement(3877..>4498)
                     /locus_tag="PLD15_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64221.1"
                     /translation="MFGVQKSMIIASSAIAISALIGTMLGLISGYLSGATDLVIMRIM
                     DSFFAFPSLILALFIIALFGSNMVNLVFAIGLVYVPIFARTVRGAALSLRESLYVKAS
                     RALGKKSLSIMLTDILPNVSSVLIVTFTTNVSTALLTEASLGFLGLGVLPPEPTLGGM
                     VGQGTAFLLSAPWVVLFPGLVIALIVLSLNILGDGLRDILDPRINR"
BASE COUNT         1023 a         1213 c         1062 g         1200 t
ORIGIN      
        1 ccaaccgtcc ggttaacagc cggatgctct gccattgagc cacgctggaa cacattgatt
       61 attgtaactc gaaactttct ttatttcaag taggtagagt tgttttttct ctcccaccgt
      121 tgcagctgac ggctctggtt gcaagcagcg gcgtaccttc tccatcgatg tcttcgacta
      181 ctgtatcgcc gactgccacg ggggcagcta tcgacaggtt ctttatctct ttcattatct
      241 ccttgatacg tgctctgtcg atcggtctgg gtgtcttcac agagaccaga ggtaatgtac
      301 cgttggatac cccaatactg gttgtcagaa ttcttttggg catcatcaac tcgttgcggg
      361 cgtactcctc accacgggca cagcggttgc cttctattgt gtattgaact ccctcttttc
      421 tgacggatac tttgcaccct actgggcata tcacgcacgt tatgtgacct tccatcactg
      481 cacctctact gtgagtttgc cgtaattcat cagcggttgt agtttgtctc tgttcactac
      541 cagctgtatc atctcgctgg gtactccgta cgagaacttc ttttcgatct ttagttcctt
      601 caggaccact acgccttttt ccatcggttt tctcagtctt atgtatagtc tcaaatgctc
      661 ctccatatcg acgattcccg ggaccagaac gcttatgttc tttccccgga agaccggcac
      721 tctatctcct gaagtattaa ctcccctgga gaatagtgcg gcgtgacgac ctgcaatcca
      781 gccttcagcg gctacataat ccaccagatc gaagatggcg acattgtttc cggccgcgaa
      841 gactccttct gtgctgcttt cacaggtgtt tgaaacgagg aagcccctgt tcatcggatc
      901 gacttcaagg tcgtcgctga aatcttctac ctgcggtaga aggccggccg aaagaataag
      961 tgcatctacg tcgatggttc tggaagtacc cggaactggc gtaaagttct cgtcgacctt
     1021 cgagatctcc acacttctca gtcttccgtc gccactgatt cccgtaaccg tggtagagag
     1081 atggagcggt atatcgaagt cttccaaaca ctgtactatg tttctagtca gtcctcctgg
     1141 gtaagacatt ctttctagaa cgcttactac ctcaactccc tccagagtga ggcgtcgcgc
     1201 cataataagt ccgatgtctc cggagcccag aatcatagct tttctggccg gtattcgatt
     1261 ttcaaggttt acgtatctct gcgcaactcc ggccggcatg acgccggccg gcctgtcgcc
     1321 cggaattaga agcgatccga aaggtctctc tctggcgccg gtggagataa ccagagctcc
     1381 ggcctgtatt tcgtaggcac cgtctggaga aagtacctgt gcgactctct ttcgaacgtc
     1441 gatatcgcgt acgtacgact ggctgatgat ttttacgttt tccatgttca tatcatcgat
     1501 cagcctggaa gcgaactccg ggcctgtgag ttcctggtga tagaattgca ggccgaatcc
     1561 gttgtgaatg cactggttca aaacgccccc cacactcgat tctctttcga gaagcgttac
     1621 atatacacct tctttcgcgg cgctcagggc acaggccata ccggcagctc cgcctccgat
     1681 cacgagaaca tccgttttct ctctcttcag catctttctc tcaccttccc gtcggttatc
     1741 cagctttcag gaacgttctg acgtatctcg gagagatctt tccccagttc tcttgagagt
     1801 atcgaggcga tcttccagct gcaaaaacca ccctgacatc gaccaaaacc ggccctggtt
     1861 ctaaacttaa ctccatctat cgttcgcgcc ccgtctctta tcgactgaac gatttcagct
     1921 tccgaaactt cgttgcactg gcagattatc tttccgtata ggggatcact ttcgatcaag
     1981 ctggagatgg cttcaaatgg gagttccttt attctggtct gttcttctat gaaaggatcg
     2041 aagtttctct tctcctcaag tgaaacgcca atagaaggca gcagctcaag aacgacgtat
     2101 tccgcgatcg cgggagcagc cgtcaagcct ggcgatctaa tggctccaac ggttatgaag
     2161 ttatcaatct cacaagcggc ttttatgtag aagtccttgc cggagctttc cggtctgata
     2221 cctgcgaagg atttgacgta ccattggggt gaatcgattc ctggaatcag ctcctttcca
     2281 gattgcctga cagaatacaa tccctccgcc gttgtcttca gctcttgcgg agtgaaatcc
     2341 tgtagctctt ccgaagtggg tccgaggagt acgccaccgt cgatcgtggg cagcaccaat
     2401 cttccctttc ctgaggcggt agggagcgga aagacgattc ttctgacaag atcgctggct
     2461 cttttatcca ggagaatata ctcgcccctt ctcaatttga ccggtggtac ctcaacatca
     2521 aaaagccgtg ccaccttttc gtagaagagg ccggttgcgt ttatcactat atcgatgttc
     2581 tttatttctc tgccggaagc cagacggagg ctctttatag agccttcttc aaccctgccg
     2641 ccgaccactc cgtctccggt gataatttca gctccgttgc gagcggcgtt tatcgaggcc
     2701 gctatggcta ccatccatgg ctcggtgata ccggcgcttc cacagtaaag ggcgtagttg
     2761 aacacactcg agatagccgg ctccagctcg tgcagttcct ctctccccac tattctcaga
     2821 tcgcgaacgc cgttagcttg gccgttgacc agtagctctt ccagtttttt caactcttct
     2881 tcgtttcttg cgacgacgat tgagccgatc ctgttgactg gaactttcaa ctctctggcc
     2941 aacctatcgt acagttcgtt gcccctggcg cacaacctgc ctcttaggct tgccggcgga
     3001 tcatcgtacc ccccgtgaat gatggccgaa tttgccttgg tgataccctg gcccagatcc
     3061 tctctcttct ctatcaggat tacttcacct tcgaatttac tcagttgccg ggcaattagc
     3121 gtcccgataa ctccccctcc aattaccacc gatctcatcg aaacacctcc aaagaaaaac
     3181 cacgccacga aagcatggcg tggttttctc ataagctcta ccacgtgtcg ataaaattat
     3241 agcatatagg actttctctt tttgcgcggc tggaagaatg gtcggagaaa acccgggctt
     3301 tttaagatgg tagaatattg gcaaggaggt taatccatgc tcaaaggtat tattgccgct
     3361 ttatgggata ggaaaacccg gccggaggag gtagatccgc ggacggtttt ctttctgaac
     3421 ggaggcctct tcgaaattca agaccggata aatgaattca aggccgccgg caagtctgtt
     3481 ttcgtcgata tagatttcat atcaggtctt tcgggcgacg aagacagtgt tctcttttta
     3541 aagaaatgcg ggatcgatgg tataatcacc gccaaaataa ggataagcaa gcaggcaatc
     3601 tctctgggaa tgaacagtct cttgagattc ttcgccctgg actcacgggc ggtggacaag
     3661 ggaattcagc agatactctc caacggtgta aaaaacatag aaattctacc aggtctggta
     3721 tcgaccaagg ttgcacccag aatacgcgcg atggttccgg atgtgaacct ggtggcggcc
     3781 ggtcttatcg ataccagcga ggagttagat cttctgaaga agtatgttga cggtgtttct
     3841 accagttcga cagttctatg gaggtataag tggtgattat ctgtttatcc tgggatcgag
     3901 tatgtccctc aatccgtcgc ccaggatatt gagactgaga actatcagag ctatcaccaa
     3961 gccgggaaag agtactaccc acggcgcact caaaaggaag gccgtgccct gtcccaccat
     4021 ccctcccagt gtgggttctg gcggtagtac tccaagaccg agaaagccaa gagaagcctc
     4081 cgtcaggaga gccgtcgaca cgttggtagt gaaggtcact atgagaaccg aagagacgtt
     4141 gggcagaata tccgttaaca ttatagaaag gctcttcttt ccgagagccc tcgaagcctt
     4201 tacgtagaga ctctccctca gggagagtgc agcccctctc acggttcttg cgaagatggg
     4261 aacgtaaacc agccctatgg cgaatactag attcaccatg ttcgatccga agagagcaat
     4321 tatgaagagt gccagtatca aggagggaaa ggcgaagaag ctgtccatta tcctcattat
     4381 caccaggtct gtggcaccgc tgagatagcc tgatatcaag cccagcatgg taccgatgag
     4441 ggcagatatt gcaatcgcgc tggaggcgat tatcatactc ttttgcaccc cgaacatg
//
LOCUS       DAOOLY010000214         4459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23536_length_4459_cov_4.589010, whole genome shotgun sequence.
ACCESSION   DAOOLY010000214 DAOOLY010000000
VERSION     DAOOLY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23536_length_4459_cov_4.589010"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLD15_09685"
     CDS             complement(<1..1150)
                     /locus_tag="PLD15_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64222.1"
                     /translation="MEEIFLSVRNVQKTYRSNGVRALDGVSLDTYAGEVNVLLGENAA
                     GKTTLMKIIAGIERADDGEMFMKGRNYSPANPVDAIRAGVGLVSQKLRVIPQLTVGEN
                     LLLGMDLSGFERKKGRKTIEEAVHRFGLGIELPVRVEDLSIAQRQKVEILRVLIRSPE
                     LIVFDEPTAVLPDNDREELMNIIARLKEGGKTVIFITHKLQEAYRIADRISIMAKGKI
                     TGTFLKGEVTERSLRTLVAGEDLAASGFSGRKTTATGEVLRVESLDVEGRYRNLIAVK
                     NVSFVSRAGEILVITGISGNGQEELIESLFGMRRVLSGRVYLEDTDITGWTPADLRKI
                     GVVALIGDRDTTASCPGLSIIDNVVLNRVRDRFFLKEKPLKTWTRELLS"
     gene            complement(1267..>4459)
                     /locus_tag="PLD15_09690"
     CDS             complement(1267..>4459)
                     /locus_tag="PLD15_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB64223.1"
                     /translation="IVVGPDLKIHECGIPKRVAMELFKPFVLSRLLHGSESSKTARKL
                     KKTIIEKEMPEAWEVLEDVIKGHPVLLNRAPTLHRISIQAFIPRLVEGNAIQLHPLVC
                     APFNADFDGDQMAVHVPLSAAAQAEAKFLMLSRYNVISPASGKPISMPGKDIIVGVYY
                     LTTVDQQYEKMEKEIMGYYAQLLKDEKVTRVEARRRVREKALEWLDWKFSSTGEALMA
                     YDTGFISLHDPILVKLDPDPTVGELTLTTVGRIIFNTIVPEKLRDYSKKFNKKAIKKL
                     IYDCFHEYGIDRTANLLDDMMSLGFHYATYSGLTISIKDIVVYPEKDKVIAAAQKRVN
                     QVESLYRSGFLTDEGRSQEIIRIWSDTTGDIMDKTYSEFEKYPFNPIFMMVDSGARGN
                     IDQLKQLAGMRGLMADPSGKTIEVPIKSNYRDGLTELEFFTSTHGARKGSADTALRTS
                     FAGYLTRRLVDVSQTITVTTPNCGTHEGIEAIELLADGVTIEKLEDFLFGRVLATDVI
                     DPRTGKVLKNSKTGKEYSRDTMIRDEDASFLSSFSTEVDVVREDVLNLSKVTRLASYT
                     ELNEDIKLGEKSHKSGTHIGSDLLHQLKNSDLGEVKVMTYPCVGQVFAGTTVKNGNNE
                     TIVKYQEKIDELTAKKLQANETGKILVRPLIKVRSVLTCESDNGVCAMCYGMDLSNHE
                     IVNVGESVGVVAAQSIGEPGTQLTMRTFHTGGIATGQDITRGLPRAEELFEARKKLKD
                     PEALFIEKDDGGFVKDLVSDEKGRRKVYVETKDGRISEYDLSSAIKPKIEIGDKVVEG
                     EAITTGTVRPRKLMEKLGLVPTALYLLTEIKRVYAEQGVEIHDKHFELIIRQMLSKVE
                     LTDPGDTDFLPGDLLDIVEVRKINKTIEEANSKVQENREYVMDKRLARRVLGEDSDGK
                     IQKIASEGEPITEELLGKILKSGIKQISIYDVEEDQYQKLIEDLNPDTVIPEKKVQIM
                     PKDLMKFKRKLLRITKASLESEGWLSAASFQQTPQILTEASVAGKCDYLLGLKENVIV
                     GQLIPAGTGLDMYANLQVEEAAVSAQFEEEELA"
BASE COUNT          898 a         1241 c         1038 g         1282 t
ORIGIN      
        1 tcgaaagtag ctcgcgggtc catgttttca acggtttttc ttttagaaag aatctatccc
       61 ggaccctgtt tagaacaacg ttatctatta tcgaaaggcc cggacagctc gcggtcgtat
      121 ccctgtcacc tatcagggct acaactccga tctttctgag atcggccggc gtccaaccgg
      181 ttatatctgt atcttcgagg taaacacgcc ccgaaagaac tcttctcatg ccaaataagc
      241 tctcgataag ctcttcctgg ccgtttccag agataccggt tataacgagt atctcgcccg
      301 ctctggagac gaaggagacg ttcttcaccg ctatcaggtt tctgtaccga ccctcaacat
      361 ctagcgattc gacccgaaga acctctccgg tggccgttgt ctttcgaccg ctgaatccag
      421 atgccgccag gtcctctcca gccaccaggg ttcttagcga cctctccgtc acttctcctt
      481 tgaggaaagt tccggttatt tttcccttag ccatgatcga gattctgtcg gcgattcgat
      541 aggcttcctg cagtttatga gtaataaaaa tcacggtttt tccgccttct ttgagcctgg
      601 ctatgatatt catcaattct tctctgtcgt tatcggggag aacggccgtc ggttcatcga
      661 agactatcaa ttccggagac ctgatcagta cacgaagaat ctcgaccttc tgcctctgag
      721 ctatcgatag atcttcaacc ctgacgggga gttctatacc cagaccgaac ctatgaacgg
      781 cttcttcgat cgtcttgcgg cctttcttgc gctcaaagcc cgataaatcc atacccagaa
      841 gtagattctc accaaccgtt agttgcggta ttactctcag cttctgtgaa accagaccga
      901 caccggcccg gatggcgtca acgggattgg ccggagagta gttcctgccc ttcataaaca
      961 tctcgccgtc atcggccctc tcgatcccgg caattatctt catcaatgtg gtttttcccg
     1021 ccgcgttctc acccagaagc acgttcacct ctccggcgta tgtatccaga gaaacaccgt
     1081 cgagggctct gactccgttg cttctatagg ttttttgaac attgcgcacg cttaggaata
     1141 tctcttccaa ccgactcacc atttcccgac tttccggcaa atttcatatg ctcattgata
     1201 atgttaccat caaagaataa aaaagcggga agccgttttt cagcttcccg caacaggatt
     1261 atcttttcag gccagctctt cttcttcgaa ctgcgcagat acggccgctt cttcgacctg
     1321 tagattcgcg tacatatcga gaccggttcc ggccggtatc aactgcccaa ctatcacgtt
     1381 ctccttaagg cctagcagat agtcgcactt gccggctacg gaggcctcgg taagaatctg
     1441 cggtgtttgc tggaacgatg cggcggatag ccagccttca ctttccagag aggccttggt
     1501 tattcgcagg agcttcctct tgaacttcat gagatctttg ggcattatct gcaccttttt
     1561 ctcgggaata acggtgtccg ggttcaggtc ttcgatcaac ttctggtatt gatcctcttc
     1621 gacatcgtat atcgagatct gcttgatacc gctcttgagg atcttgccaa gaagttcttc
     1681 agttatcggc tcgccttcgg aagcgatttt ctggatcttt ccgtcactgt cttctcccag
     1741 aacccttctg gcaagtctct tgtccataac atactctctg ttttcctgga ccttgctgtt
     1801 ggcctcttcg atcgttttgt ttatctttct aacctctact atgtccagaa gatcgcctgg
     1861 caggaaatcg gtatcccccg ggtcggttag ctcgaccttc gaaagcatct gtcttattat
     1921 cagctcgaaa tgcttgtcgt gtatctcgac accctgttcg gcgtagaccc tcttgatctc
     1981 tgtgaggagg tagagggcag tcgggacaag acccagtttt tccatcagtt tccttggtct
     2041 gacggttccg gtagtgatcg cttctccttc gacgacttta tctccgatct ctatcttcgg
     2101 cttgatggcc gatgagagat cgtactcgct gatacggccg tctttggtct cgacgtaaac
     2161 cttgcgtctt cccttttcgt cggacaccag atcttttacg aagcctccgt cgtccttttc
     2221 tatgaagagg gcctcgggat ccttgagttt ctttcgtgcc tcgaaaagct cttcggccct
     2281 tggaagacct ctggttatat cctgtccggt cgcgatgcct ccggtgtgga atgttctcat
     2341 agtcaactgt gtacctggtt cgccgatcga ctgcgccgca acgactccta cggactcgcc
     2401 gacgttcacg atctcgtggt ttgaaagatc cattccatag cacatcgcac agacaccgtt
     2461 gtctgactcg caggtcagaa ccgatctcac cttgatcaac ggtctcacaa ggattttccc
     2521 ggtttcgttg gcctgaagtt tcttggcggt cagttcatct atcttttcct ggtatttgac
     2581 aatcgtttca ttgttgccgt tcttcaccgt agtgcctgcg aacacctgtc ctacacacgg
     2641 gtaggtcatc acttttacct ctccgagatc ggagttcttt agctggtgaa gaagatcgct
     2701 gcctatgtgg gtaccggact tatgagactt ttcaccgagc tttatatcct cgttgagctc
     2761 ggtgtagctc gccagtctgg tcaccttgga caggttcaga acgtcttcgc gtaccacatc
     2821 gacttccgtg ctgaaactcg agaggaagga ggcgtcttcg tcccttatca tggtgtctcg
     2881 cgagtactcc ttaccggttt ttgagttctt cagcacctta ccggttctgg ggtctataac
     2941 gtcggttgcg agaacccttc caaagaggaa atcctccagc ttctctatcg tgacgccgtc
     3001 ggccaggagc tctatcgctt cgatcccttc gtgggtaccg cagttgggcg ttgtaacggt
     3061 tatcgtttga gaaacatcta ccagtctcct ggtcagataa cctgcgaacg aggtcctcaa
     3121 ggcagtatcg gcagaaccct ttctggcgcc gtgcgtcgag gtgaagaact cgagttctgt
     3181 caaaccgtct ctgtagttcg actttatcgg aacttctatg gtctttccgg aaggatcggc
     3241 catcaatcct ctcatccccg ccagttgctt gagctggtct atgtttcctc tcgcacccga
     3301 atcgaccatc atgaatatcg ggttgaaggg gtacttctcg aattcgctgt aggtcttgtc
     3361 catgatatcg cccgtggtgt ccgaccagat acggattatc tcctgactcc tgccctcatc
     3421 ggtcaaaaag cccgatctgt agagactctc gacctggttg accctcttct gggcagccgc
     3481 gattactttg tctttttcag gatatacgac tatgtccttt atcgagatgg tcagaccgga
     3541 gtaagtggcg tagtggaaac ccagcgacat catgtcgtcg agaagattgg cagttctatc
     3601 tatcccgtac tcgtggaaac aatcgtagat gagcttcttt atggccttct tgttgaactt
     3661 cttcgaatag tctctcaact tctcgggcac aatcgtgttg aagattattc tcccgaccgt
     3721 cgtgagcgtg agttcaccga ccgtgggatc ggggtccagt ttgaccagta taggatcgtg
     3781 cagcgatatg aaaccggtat cgtaggccat cagcgcttcg ccggtcgagg agaacttcca
     3841 gtccaaccac tccagtgctt tctctctaac ccttctcctc gcttcgactc tggttacttt
     3901 ctcatctttc aacaactgag cgtaataacc cattatctct ttttccatct tctcgtactg
     3961 ctgatcgacg gtcgtgagat agtaaacccc gactattatg tcctttcccg gcatcgagat
     4021 cggtttcccg ctggccggtg aaatgacgtt gtagcggctg agcatgagaa atttcgcctc
     4081 cgcctgggct gcggccgaaa gcggcacgtg tacggccatc tggtctccgt cgaagtcggc
     4141 gttgaacggg gcgcacacca aggggtgaag ctgtatggcg tttccttcga ccagtctggg
     4201 tatgaaggcc tgaatcgaga tcctgtggag cgtaggagcc ctgttgagaa gcacgggatg
     4261 tcctttgatg acatcttcaa gcacttccca ggcttccggc atttccttct ctattatcgt
     4321 tttcttgagc tttctcgcgg tcttgctcga ctcgctacca tgaagcagcc tggagagaac
     4381 gaagggtttg aacagctcca tcgccactct cttggggatt ccacactcgt gtatcttcag
     4441 atccggtcct acaacgata
//
LOCUS       DAOOLY010000215         4428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23738_length_4428_cov_5.214041, whole genome shotgun sequence.
ACCESSION   DAOOLY010000215 DAOOLY010000000
VERSION     DAOOLY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23738_length_4428_cov_5.214041"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2569)
                     /locus_tag="PLD15_09695"
     CDS             complement(<1..2569)
                     /locus_tag="PLD15_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64224.1"
                     /translation="MGCPVFDENRVNGDRPIKLLLPLLFLILIPAFLIAADFELILST
                     PPPGQVRPGEIVTLAVLVTNNSQDRLNLSEELLLPSEWRNVFGTSSFEIEPDSFDIRL
                     LSFLVPARTLAGDYQLSYRVKSDDGRVSGLNFTVKVASNVAIRVELIDGPSYVRAGEG
                     YSLVFLITNNGNIPVALNLRVNDNLSYALKSSVSKLELEPARSGRVTVEVKTSRTLLE
                     QNNHIVILIASIESGDGGSVSGRSSVLLLPPIDSLASRFRLLPARVSITTGYSQSFFL
                     AAGLRMEGFLNDEKTRSVSLNTSIPVTGANRDNRFEADLRYRGEILEAGYGSLPAAGQ
                     ASTPVGTSTRGFDLQLKLDSLKLGMIVSNSLSRYGLFIDWRPTQELLASFAFDRNLDS
                     SGPSYASVVLESDEKPVGARSVLRVPFVEGKFDGILLDYTFVSRLESWQLRSSIGYSK
                     RLTGPSNNELLKLDVGATWSRKDSLRVSLEGTVQRNNPFDFMLPGVLSGYSLRARVDL
                     PAMSGLRVSLGAHYVSSGTRNPASTVRNYGGNLSLAYSISILNVYGSLSGQASQIGGL
                     LKSTLQLDIYGSYRFSDLAYLSARITLKNYVGEWFETTAALSGSYIFESGTRVNLSLG
                     YAMKDLTPSNISFGVALSKSFVDGGNFTASAAVSIAERDSWKPAFEFKAGYSFPFSLP
                     VWPRNDLASLSGKVVVIEDGKERPIVGVVVRLNNLVAITGKEGEFAFKGIRPGTYYVE
                     IDGQSIERGLVSVDKLPLKLELKADEESGISLGFLKSSTLRVRVINEEKEPVNLGGIY
                     FTLSNGEENFRLSTRENGEISVPNLRPGKWTLRIDGDTLPRGFVIDNQTAELELKSGS
                     "
     gene            complement(2536..3315)
                     /locus_tag="PLD15_09700"
     CDS             complement(2536..3315)
                     /locus_tag="PLD15_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64225.1"
                     /translation="MIKKIVLLIFILAFISVKADILIINGLTHIFQASPGQVLSGVIE
                     LQNNSMDPARVRIYLSDYFFYHTGSTEYPEAGSLQRSNANWIELQAPEITVPGNANLT
                     YNFSAVVPSDESLSGTYWAVIMVEPTDDFKDDEVEGITVRTKTRYAIQVVTNFGEPQA
                     KSVAILGRSLSKNNGLYSLNMDIQNTGNWWMRPELSASVFNQSGETVGEFMGNSMRLY
                     PGTSGHFVVSFVDLAPGDYRVLALIRDGEDVWGAQYSMKIE"
     gene            complement(3353..3922)
                     /locus_tag="PLD15_09705"
     CDS             complement(3353..3922)
                     /locus_tag="PLD15_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64226.1"
                     /translation="MSLLPGTYLALKQRKALLLCLGVLIFAVSTGAVTLSATGKVTGD
                     IAWEIIAVSPELAGDGPVLNASSQMVSPADSTKIVVNRPEITGGYGQERKYRVIVTRS
                     SGMKISLRLSEIPVAWTPSNQSGSVTPLYTGYLELSMAGVSYDFVDIVFRPRGEIVTF
                     YIQYALDPDDLDLMGGTSITFTVIDMGNL"
     gene            complement(3931..>4428)
                     /locus_tag="PLD15_09710"
     CDS             complement(3931..>4428)
                     /locus_tag="PLD15_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64227.1"
                     /translation="FRRILRPLRPNYAHEEDPRKSPGLGRHRKKRPRRAVTVVQDPTY
                     LRYTSLRFGGLERKVTTTFSTLPAGFSISLAAGTTDSSAKGNVGTGATLEFVGTTLTG
                     DLVTSIGAGLARTGIESNAGHPLTYTAMIDETALANLGDGLADGGFLNAVDTVITVTF
                     TLTDI"
BASE COUNT         1113 a         1181 c         1057 g         1077 t
ORIGIN      
        1 cacttcccga tttcagttcg agttcggcgg tttgattatc tatcacaaaa cccctgggca
       61 gagtatcgcc gtcgatacgc agagtccatt ttcccggcct caggtttgga accgagatct
      121 cgccgttctc cctggtgctc aatctgaagt tttcttcgcc attactcaac gtgaaatata
      181 tcccgcccag attgacgggc tccttttcct cgtttattac tcttaccctc aaggtcgaag
      241 acttaagaaa gcccaacgat atcccggact cttcgtcggc tttcagttca agcttaagcg
      301 gcagcttatc gacgcttacc agtcccctct caattgactg gccatctatt tccacatagt
      361 aagtccccgg ccttatacct ttgaaggcaa actcaccttc cttgccagtg atggcgacca
      421 ggttgttgag ccttacaaca acccccacga tcggtctttc tttcccgtct tcgataacta
      481 ccacctttcc agatagagaa gccagatcgt ttctgggcca tacgggaagc gagaacggaa
      541 aggaataacc ggccttgaat tcaaacgccg gcttccagct gtcccgttcg gcgatagaca
      601 ccgccgcgga tgccgtgaag ttacctccat ccacgaagga tttagaaagc gcaacaccga
      661 agctgatatt tgaaggagta aggtccttca tcgcataccc cagcgataag ttcactctgg
      721 tgccgctttc gaatatataa gaacccgaga gcgccgctgt cgtttcgaac cactctccca
      781 cataattttt cagcgttatt ctcgccgata gatacgccag atcggagaac ctgtaggatc
      841 cgtaaatgtc caattgcagc gtcgatttca aaaggccacc tatctggctt gcctgtccag
      901 acagagagcc ataaacattc aaaatcgaaa tagagtaggc cagcgagagg ttaccaccgt
      961 agttcctgac tgttgaggcg ggattcctcg taccgcttga gacgtagtga gctcccaggc
     1021 taactctcaa acccgacatc gctggaagat ccacgcgagc acgcaaagaa taaccgctca
     1081 atactccggg caacataaaa tcgaagggat tgtttctctg taccgtccct tcgagggaga
     1141 cccgtaaaga gtccttcctg ctccatgtgg cacctacatc tagcttgagt agctcattgt
     1201 tcgatgggcc ggtaagcctt ttggagtaac ctatcgaaga tcttagctgc caactttcga
     1261 gacgactgac aaaagtgtaa tccagcagga taccatcgaa tttaccctcg acaaagggga
     1321 cgcgcaaaac cgaccttgca ccgacgggct tctcatcgga ttcgagaacc acggacgcgt
     1381 aagaaggacc ggaggagtcg agattcctgt cgaaggcaaa gctcgccagc agctcttgag
     1441 taggtctcca gtcgataaaa agaccgtacc tggacaggct gttcgaaact atcatcccga
     1501 gttttaaaga atcgagtttc aactggagat cgaagcctct tgtggaagtg ccgactggtg
     1561 tcgatgcctg tcctgccgcc gggaggcttc cataaccggc ttcaaggatt tctcctctgt
     1621 accgcagatc cgcctcgaat ctgttgtccc tgttggcacc ggtcacaggg atggaagtat
     1681 tcaacgacac gcttctagtc ttttcgtcgt ttaaaaagcc ttccatcctt agaccggctg
     1741 ccagaaagaa gctctgcgag taacctgtgg ttatcgatac tctggctgga agcaacctga
     1801 acctgctggc cagagagtcg atcggtggaa gcagcaagac cgaggaacgg cccgaaacgc
     1861 ttccgccatc tcccgactct atgctagcta tgagaattac gatgtgattg ttctgttcca
     1921 gaagcgttct ggatgttttg acttctaccg ttacccttcc cgatcgggcc ggttcaagct
     1981 cgagtttgga tacgcttgat ttcaaagcgt acgacaggtt atcgttcact ctcaaattaa
     2041 gcgctacggg aatattgcca ttgttggtga tcaaaaaaac cagcgagtac ccctcgccgg
     2101 cccgaacata cgagggaccg tcgataagct caacccttat cgccacattc gacgcgacct
     2161 tcactgtgaa gttcaacccg gatacccttc catcgtcgct tttgaccctg taactcaact
     2221 ggtaatcacc ggccagcgtt ctggccggta caagaaaact caaaagcctg atatcgaagg
     2281 aatccggttc gatctcgaaa gaagaggtcc cgaatacatt tctccattct gaaggtagca
     2341 gcagttcttc gctgagattc agtctatctt gcgagttgtt ggtcacaagc acggccagag
     2401 taactatctc tccaggcctg acttggccgg gagggggtgt agaaagaatg agttcaaagt
     2461 cggcagcgat cagaaaagcc ggtatgagaa ttagaaagag aagaggtaaa agtagtttta
     2521 tcggccggtc tccattcact ctattttcat cgaatactgg gcaccccata catcttcacc
     2581 gtctcttatc agggccagaa ccctgtaatc gcccggggcc aaatcaacga acgaaaccac
     2641 gaaatgcccg gaggtccctg ggtacagtct catcgaattt cccatgaact ctccgacagt
     2701 ttcaccggat tgattgaaga cactcgcgga aagctccggc ctcatccacc agtttccggt
     2761 gttctgaata tccatgttca gcgagtaaag gccgttattt ttcgatagac ttcttcctaa
     2821 aatcgcaacg ctcttggctt gaggctcccc gaagttggtg acaacctgaa tcgcgtagcg
     2881 tgtcttagtt ctgacggtaa ttccctctac ttcgtcgtct ttgaagtcat cggtgggttc
     2941 gaccatgatc actgcccagt aagttccaga taagctctca tcggacggaa cgaccgccga
     3001 aaagttgtaa gtcaggttcg cgttgcccgg aacggttatt tcaggtgcct gaagctcaat
     3061 ccagttcgca ttggatcgct gcaggctacc ggcttcgggg tattccgtcg aacctgtgtg
     3121 gtagaaaaaa taatccgaca gataaatacg aacacgtgcc gggtccatcg agttgttttg
     3181 cagctcgata actccggaca aaacctgtcc gggagaggcc tgaaaaatat gtgtgagtcc
     3241 gtttattata aggatatctg cctttacgga tataaatgcg agtataaaaa ttaacagtac
     3301 gattttctta atcatatgaa tcagctccaa atcaacagca ctaccaaagg tattataggt
     3361 tacccatatc gattaccgtg aaagtaattg aagtgccacc catcaagtcg agatcatccg
     3421 ggtctaaggc atactgaatg tagaaggtca cgatttctcc ccgaggtctg aagactatat
     3481 ccacgaaatc gtaagatacc ccggccattg aaagttcgag ataacctgtg tagagtggcg
     3541 tgacagaacc gctttgattc gaaggagtcc acgctaccgg tatttctgac aacctcagcg
     3601 atatcttcat accggaagaa cgggtcacga ttactctgta ctttctttct tgtccgtaac
     3661 cgccagtgat ttccggtctg ttcaccacta tcttcgtgga atctgccgga ctcaccatct
     3721 gcgaagacgc attaagcaca gggccatccc cggccaattc cggagacacc gcgatgattt
     3781 cccaggcgat atcgcccgta actttacccg tggccgataa cgtgaccgca ccggtcgaaa
     3841 cggcgaaaat cagaacacca agacagagaa gcagcgcttt gcgctgcttc aatgccagat
     3901 atgttccggg aagaagactc atctttcaga tcagatatcc gtcagagtaa acgtgacagt
     3961 tattactgta tctactgcgt tcaagaaacc accatccgca aggccgtctc caaggtttgc
     4021 aagtgcagtc tcatcgatca tggctgtgta ggtcagggga tgaccggcgt tggactctat
     4081 gccagttctt gccaggccag ctcctataga agttacgaga tcaccagtta gagttgttcc
     4141 tacaaactca agcgtggcgc cggtccctac gttacccttt gccgaagagt cggtggtgcc
     4201 tgccgccaga gatatggaga aacccgcggg aagagtgctg aaggttgtgg tgacctttct
     4261 ctcgaggcct ccaaatctca gggatgtgta tctgagatat gtagggtctt gtacaaccgt
     4321 gacagctcgg cgaggtcgct ttttgcggtg gcgcccaagg cccggcgact ttcgaggatc
     4381 ttcttcatgc gcgtaattcg gccgcaaagg tcgcaagatc cggcggaa
//
LOCUS       DAOOLY010000216         4406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_23910_length_4406_cov_5.450011, whole genome shotgun sequence.
ACCESSION   DAOOLY010000216 DAOOLY010000000
VERSION     DAOOLY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23910_length_4406_cov_5.450011"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..698
                     /gene="rimP"
                     /locus_tag="PLD15_09715"
     CDS             240..698
                     /gene="rimP"
                     /locus_tag="PLD15_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPB64228.1"
                     /translation="MEDLKEELRELAEEVAKSLGFSVYDFSFSRRGRKRLLKVTIDKL
                     EGYVSIKDCEAFSNSFGKALDTADIVPFPYDLVVESPGVERALRNMDEFKRFTGEKVK
                     IVFNEPLNGNSTLSGKILSCEGKKIVVESADNQMEVVISFENVKRANLKL"
     gene            721..1749
                     /gene="nusA"
                     /locus_tag="PLD15_09720"
     CDS             721..1749
                     /gene="nusA"
                     /locus_tag="PLD15_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPB64229.1"
                     /translation="MNLNLLEALDQLEQDKGIEKDEVINILEKALQSAYKKNFASESD
                     VEVHIDRLTGDIEVYEMLTIVEEIENQATETTLEEALKISSGAEIGATIERKLNIKKF
                     KRIAAQTARQVLIQKIREKEKENLFDKYVDIKGTVSTSEVLRITPEWVDLRIGKIETR
                     IPTKELIPGEQPRLNSLMKVYVVDVSKSSKGPRIMVTRKMPEFVVELLKLQVPEIASG
                     DVTVKSIAREEGIRSKVAVYSQNAKVDPVGACIGESGTRIAEVLREIKPEKVDILRWS
                     NDPAELVGNSIAPATALEVRVVSSQNREAVVYVSPTQLSLAIGKGGQNARLAAKLTGW
                     KIDIKPIM"
     gene            1776..2243
                     /locus_tag="PLD15_09725"
     CDS             1776..2243
                     /locus_tag="PLD15_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPB64230.1"
                     /translation="MKCDQLRTELKKRNQRMTAQRDIVLKSFIEAETDHLSADEAYQK
                     VMEKNCRISKATVYRTVELLAGLGLLRRIVFRDGIIRYELVKKGEHHHHHVVCNRCGS
                     VVEFPLDNLDDLEELVREKTGYTIEDHQLKFYGLCPKCQSEKKTQNNRDSETK"
     gene            complement(2174..3520)
                     /locus_tag="PLD15_09730"
     CDS             complement(2174..3520)
                     /locus_tag="PLD15_09730"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPB64231.1"
                     /translation="MKSNTEFPLIYYLFAIILGGLSILLAFSSPAGTIALLSLSTAAF
                     LISQRSVNRFLWLAFFLAGLSLQMMVPRKIEPGGIEYTGYVTRGGTGFVEASEGKILI
                     NGNWRSLRDTVQIRLSKSNEELYTGQIIWAAGNMEVRSIFPLYVIDSVADGHFERFRL
                     LSLPGRYSRDKLKKYGLLDTMAGSVFLGDRRSMESGTRNEVSNLGVAHLFAVSGLHIG
                     MMYLFANYALSRFLLGRRTKLMLSVSLIFLYTMATGPSISAMRSFLMLAIYSFFKFID
                     YRQHPLNVLGLTGILMMLSQPSIIVSISFQLSFFATAMLLIFLPVFTEHNFMFQAFMT
                     GVIAQIAVIPLSLNVFGTISVASIPLTILMVPLFIIPSYIGVISLMIFDALNFGPICD
                     FLAGALKQLSRIFEISTETISGLIPVMRLEGVGAYLVSLSLLFCVFFSLWHLGHKP"
     gene            3632..>4406
                     /locus_tag="PLD15_09735"
     CDS             3632..>4406
                     /locus_tag="PLD15_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPB64232.1"
                     /translation="MRQFPFGGLTLVGYYGYDNLGDDLLLLSSLTLLEEIEFSGPVFL
                     PAATPVEKVSGRFPEKLDIRIIKRFDPRELALAIGRSVMTVFGGGNLLQDCTSWRSFI
                     YYYGLARYTLRKGKPLLFLSQGFGPVEHRSNKKALDRILSNPLTSGVMRDDCSFQHFY
                     SLSKNVHPGTDYGPYYLFKKEILPKRRRSETGLAVIVLKNGTKVDDVLKALKLNNITA
                     VCAIGFHNHHDEEKRRELEEKARVNGFKIYKTSTDMEGVI"
BASE COUNT         1281 a          965 c         1169 g          991 t
ORIGIN      
        1 ggatttcacc ataggtgagg tcctttatcc tgttctgata tcgctgctga tctcgatatc
       61 tttgatcttc ctgtcgagtt tcatgtattc cagggagtcg atcacgatgg ccgagtagct
      121 acgaagattg actgagctgt agaaaaaagg tataatccca tgtagtaagt cgtattgtaa
      181 tactcccaaa gggagagggc ttttgtcgcc ctctattttt tattatggtg gtgttgatta
      241 tggaagacct gaaggaagag cttagagaac ttgccgagga ggtagcgaag agtcttggat
      301 tctctgtgta cgacttcagt ttttccagaa gaggaaggaa gagactcctc aaagtcacta
      361 tagacaaact ggagggatac gtatcgataa aggattgtga agccttttcc aacagttttg
      421 gaaaggctct ggatacggcc gatatagtgc cttttcctta cgatctcgtc gttgagtcgc
      481 ccggtgtcga gagggcactt agaaacatgg atgaattcaa aagattcacc ggtgagaaag
      541 tcaaaatcgt tttcaatgaa ccgttaaacg gaaattccac actttcggga aagatcttat
      601 cttgcgaagg aaagaagatc gtcgtcgaaa gtgcggataa tcaaatggaa gtggtcatat
      661 ctttcgagaa tgtcaaaaga gcaaacttaa aattatgata cataacagcg aggtgttacc
      721 gtgaatctca atctcttaga agctttggat cagcttgagc aggataaggg aatcgaaaaa
      781 gatgaggtaa taaacatact tgaaaaggcc ctgcagagtg cctacaagaa gaatttcgct
      841 tccgagagtg atgtcgaagt gcacatagac cggctaaccg gcgatatcga agtttacgag
      901 atgctgacga tcgtggaaga gatcgagaac caggctaccg agacaacact cgaggaggct
      961 ttgaagataa gctccggtgc agagatcggg gcgaccatcg aaagaaaact gaacatcaag
     1021 aagttcaaaa gaatagccgc ccagacggcc agacaggtat tgattcaaaa gatcagggaa
     1081 aaggaaaagg aaaatctctt cgacaaatat gttgacataa agggtaccgt cagcacttcg
     1141 gaggttctca gaataacccc tgaatgggta gatttgagaa ttggaaagat agagacccgc
     1201 attccgacca aagagttgat acccggtgag cagcccagac tgaacagcct gatgaaagtt
     1261 tacgtcgtcg atgtatcgaa atcctcgaaa ggaccgagga tcatggttac gagaaagatg
     1321 ccggaattcg tcgtggagct tctcaaactg caggtccccg agatagccag tggggatgtg
     1381 accgtcaaat cgatagccag ggaggaaggg ataagatcga aggtagcagt ctacagccag
     1441 aacgcaaagg tagatcccgt cggagcctgt ataggtgaga gcggaactag aatcgccgaa
     1501 gttttgcgcg agataaagcc ggaaaaagtc gatatacttc gctggagcaa cgatccagcc
     1561 gagctggtag gaaactccat cgctcctgcg accgctctgg aggttagagt cgtgagttcc
     1621 cagaaccgtg aagcggtggt ttatgtctct ccaacacagc tttcgctggc aataggaaag
     1681 ggcgggcaga acgccagact ggccgcgaag ctcacgggct ggaagatcga tataaaacca
     1741 atcatgtagt tttatctcat gaatggaggt ttatcatgaa atgcgatcag ctcagaacgg
     1801 agctgaaaaa gagaaaccag aggatgacgg cccagagaga tatagttctg aagtctttta
     1861 tcgaggcgga aacagatcat ctgagcgccg acgaagctta ccagaaagtg atggagaaaa
     1921 attgcagaat aagcaaagcg accgtctaca gaacggtcga gctcctcgcc ggactcgggc
     1981 tgttgcgcag gatcgtcttc agggacggaa taataaggta tgaactggtg aagaagggcg
     2041 agcatcacca ccatcacgtt gtctgcaaca gatgcggtag cgtcgtcgaa tttcctctgg
     2101 ataatctcga tgatctggaa gaactggtca gggagaagac ggggtacacg atcgaagatc
     2161 atcagttgaa attctacggc ttgtgcccta aatgccagag cgaaaagaaa acacagaaca
     2221 acagagacag tgaaaccaaa taagcgccca caccttccag tctcatcacg ggtatcaatc
     2281 cagagatcgt ttcggtagag atttcgaaga ttctggaaag ctgcttgagt gctccggcca
     2341 gaaagtcgca gatagggccg aagttcagcg catcgaatat cattagcgag atgacgccta
     2401 tataggaagg tatgatgaac agcggtacca tgagtatggt gagcgggata ctggctaccg
     2461 agatggttcc gaaaacgttg agggacagag gtatgacggc gatctgtgcg ataacgcccg
     2521 tcatgaaggc ctgaaacatg aagttgtgct ctgtgaatac tggcaaaaag atgagcagca
     2581 tcgcggtcgc gaagaaactg agctggaaag agatcgatac gattatcgac ggctgagata
     2641 acatcataag tataccggtg agtccgagaa cgttcagtgg gtgttgccga tagtcgatga
     2701 atttgaagaa cgaatagatg gcgagcatta ggaagctcct catcgccgag atcgagggac
     2761 cggtcgccat tgtgtagaga aagatgagcg atacggaaag catcaatttc gtccttcttc
     2821 cgagaagaaa tctggataag gcatagttcg cgaacaggta catcatgccg atatggaggc
     2881 ccgaaacggc gaacaggtgg gcaaccccca gattggaaac ctcgttcctt gtaccgcttt
     2941 ccatagatct cctgtcacct agaaagacgg agccggccat tgtatccagc aacccgtact
     3001 tcttcagttt gtctctggaa taccttcccg gcaaagagag tagtctgaac ctctcgaaat
     3061 ggccgtcggc aactgaatct ataacgtaaa gcgggaagat gcttctgacc tccatatttc
     3121 cggcggccca gattatctgt ccggtgtaga gctcctcatt gctttttgaa agccttatct
     3181 ggaccgtgtc tctaagagag cgccagttcc cgttgatcaa tatctttcct tccgatgcct
     3241 cgacgaatcc cgtccctcca cgcgtaacgt atccggtgta ctcgataccg cccggttcga
     3301 tctttcgagg caccatcatc tgtaaagaga gaccggccag gaaaaaggcc agccacagaa
     3361 acctgttgac cgatctctgc gagatcagaa aggctgcggt cgaaagcgac aggagcgcaa
     3421 tagtaccggc aggagacgaa aaggctagga gtatcgacag cccgcctaga ataatagcaa
     3481 agagataata gatcagtgga aactctgtgt tactcttcaa aagtccatcc ccccgactaa
     3541 ttgtacaacc cgtttgaaat cttttgtata ggggtatctg aaattcactt gtgataaaat
     3601 actattgtct aatgttcccg gaggttatgt tgtgagacaa tttccgtttg gcgggttaac
     3661 tctggtgggt tactacggtt atgacaacct tggtgacgat cttttgttgc tctcttctct
     3721 tacgctactg gaagagatag aattcagcgg tcccgtcttt ctgccagccg caactccggt
     3781 cgaaaaggtc tctggaaggt ttccggagaa actggacata cggatcataa aaagattcga
     3841 cccgcgagag ctcgccctgg ccataggccg ctcggtgatg accgtcttcg gcgggggaaa
     3901 tcttttgcag gactgcacta gctggagaag cttcatctac tactacggac tggccagata
     3961 cactttgaga aagggaaagc cgctcctctt tctttcccag ggtttcgggc ccgttgagca
     4021 tagatccaac aaaaaggctc tggacagaat cctctcgaat cctctcacct cgggagtgat
     4081 gagggacgat tgcagcttcc agcacttcta ttcgctttcg aagaacgtcc atcccggcac
     4141 cgattatgga ccttactacc ttttcaagaa ggaaattctc cccaaaagga ggcgaagtga
     4201 aacgggcctg gcggtgatcg ttctgaaaaa cggaacgaag gtcgatgatg ttctgaaagc
     4261 cttgaaactg aacaacataa ccgccgtctg cgcgatcggt tttcacaacc atcacgacga
     4321 agagaagaga agagagctcg aggagaaggc gagagtgaac ggcttcaaga tctacaagac
     4381 ttccacagat atggaaggtg tcatag
//
LOCUS       DAOOLY010000217         4383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_24057_length_4383_cov_5.112061, whole genome shotgun sequence.
ACCESSION   DAOOLY010000217 DAOOLY010000000
VERSION     DAOOLY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24057_length_4383_cov_5.112061"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..889
                     /locus_tag="PLD15_09740"
     CDS             224..889
                     /locus_tag="PLD15_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB64233.1"
                     /translation="MKILFADDERNMRILVADFLEMEGYKVLLAVDGEEALRKFCENP
                     DLSLIILDIMMPRMSGIEVLEEIRERCHIPVIMLTAKSAETDELEGFQCGADEYISKP
                     FSPRILVARVNALLRRSCPMQKETKIGVLHVKPSSMEVYVNDRRIVLSRTEFNLLSCF
                     VSNSGITLTRDQLLDSVWGYDYQGTDRTVDTHINRLRSKLREASEYIETIRGYGYRFG
                     VRQ"
     gene            886..2370
                     /locus_tag="PLD15_09745"
     CDS             886..2370
                     /locus_tag="PLD15_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB64234.1"
                     /translation="MSPIRRKLFLQILAVILFIISALWLANTFMLEPYYMKTKKDQLI
                     KARNSINSFGVSDYQEKVDFLVDMGNELNAMILIFNGSGEPVYTTHLIPQEPGGFVPG
                     IDTRRIPPSRKPLETVYSEKIDSGTEIELAIGRLGEQRFLSLKSRLDNGFTLHMSIPV
                     SSIESSVSVVNGFLLVVGLVSSALALVIALIISRRFTGSILHMNRITRKMKDLDFSEK
                     CEIKSKDEFGQLAGSIDDLSESLDATLKELSQKNEALKKEIEHKKAVEEMRKQFITNV
                     SHELKTPLSLIQGYAEGLELNIASSDEKRNFYCGVIKEETAKMGRLVKDLLDLSMMET
                     GNLTLYKSEFCVDELIKRVTGKFEKVFLKNGIEPKYAEGERTVVYADSFRTEQILTNY
                     LNNAVYHVDERKEIFVRKEIQENSVIVRVFNSGRNIPEEESEKIWTSFYRTDSARTRE
                     EERYGIGLSIVKAICDLDGKSCGFVNLPDGVEFFFELEKAKTSD"
     gene            complement(2367..3299)
                     /locus_tag="PLD15_09750"
     CDS             complement(2367..3299)
                     /locus_tag="PLD15_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPB64235.1"
                     /translation="MILAFLPAVFFGWALGRNDAANIYGTTVTNGLLNYRFAAITASL
                     SIIAGAIIGGRNGLETLASLSSQTLVSAAIVAAAAGLSVIILNHFGLPVSSSQAVVGA
                     IMGIGLLNGSVDWFVVAKIVICWITTPLGAALLGYVLYRIIARFFRRIKSIVVQDITL
                     KALAIVIGILGSFALGANNVANITGTFVGSISIDEAALIGGLSIAAGAIIFSKKVMYT
                     VGKRIVSLEAFSAVVAVLAQSLTVLIYAYIGVPVSVSQAIVGAVIGVGLARGSRNFDG
                     RLIRRILLGWLQTPIIAGCVSASLFAIFSFFGLS"
     gene            3711..4217
                     /locus_tag="PLD15_09755"
     CDS             3711..4217
                     /locus_tag="PLD15_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPB64236.1"
                     /translation="MYNRSEDSTRIVIGIIGGIIYAVGVGLIIFSFASFFLAPAIGTV
                     FTSMFTFVILGIVASSIGRALVGMARTRKYGGSVDDQSSPRREVQEDMTYDYTFVSDR
                     EAKTERKKPLSYSRCPECDAENDDRASTCQKCGASLVGYKKCSLCYKLNEMEKRFCSN
                     CGHDFNLD"
BASE COUNT         1214 a         1065 c         1109 g          995 t
ORIGIN      
        1 ctccatgatg tgtctgatct tgctcttgag agagatatag tcgaaacagc ttatataata
       61 cttgagttcg tgccttccct tgatctcttc catacggttc ctccagtttc atcgttgatc
      121 gattttaagg agcttctgtc acagaattat cacagcggct ctgtgataga tttgtgacat
      181 ggcgatatta gaataaagcc ataccggaca gggaggtcgt gggttgaaaa tcctcttcgc
      241 cgacgatgaa agaaatatgc ggatactggt ggccgatttt ctcgaaatgg agggctacaa
      301 ggtgcttctg gcagttgatg gcgaggaagc tctccgtaag ttctgcgaaa atcccgatct
      361 gtctctcatc attctggata taatgatgcc gcgtatgagc ggtatagagg ttctcgagga
      421 gataagagag agatgccata tccccgttat aatgctcacc gcgaaatccg ccgaaaccga
      481 cgaactggaa ggcttccagt gcggcgccga cgaatacata tccaagccct tcagtcccag
      541 aatactcgtg gccagagtga atgcgctact caggaggagt tgcccgatgc agaaggaaac
      601 gaagatcgga gttcttcatg taaagccctc cagtatggaa gtgtacgtca acgacaggag
      661 aatcgttctc agtcgcaccg agttcaatct gctcagctgt ttcgtctcca acagcggaat
      721 aacgctcaca agagaccagc tcctcgatag cgtctgggga tacgattacc agggtaccga
      781 tagaacagtc gatactcaca taaacaggtt gagatcaaag ttacgggaag cgagcgagta
      841 catagagact atcagggggt acggttacag gttcggagtt cgccaatgag cccgattcgc
      901 aggaaactct ttttacagat tcttgcggtg atactcttta tcatttcggc actgtggctt
      961 gcaaatacct tcatgcttga accgtactac atgaaaacga aaaaggatca gctgatcaag
     1021 gccaggaaca gcatcaattc cttcggagtc tccgattacc aggagaaagt cgattttctc
     1081 gtagatatgg gcaacgaact caacgccatg atcctcattt tcaacggttc gggcgagccg
     1141 gtctatacga cgcaccttat tccgcaggag cccggtggtt tcgtccccgg gatcgacacc
     1201 agaaggatac ctccgtccag aaaacctctc gaaacggtct acagcgaaaa gatcgattcc
     1261 ggcacggaga tcgagctcgc cataggcagg ctgggagagc agaggtttct aagcctgaaa
     1321 tccaggctgg acaacggttt cacccttcac atgagcattc ccgtgtcctc catcgagagc
     1381 agcgtctcgg ttgtgaacgg ctttcttcta gtagtcggac tggtctcctc ggcgctggct
     1441 ctggttatag ctctcatcat ttcaagaagg ttcaccggtt cgatccttca catgaacaga
     1501 atcaccagaa agatgaagga cctggatttc tctgaaaagt gcgaaatcaa atccaaagac
     1561 gagttcggac agcttgccgg tagcatagac gatctttcgg aatctctcga tgcgacgctg
     1621 aaagaactca gccagaagaa cgaggcgctc aaaaaagaga tagaacacaa aaaagccgtt
     1681 gaagagatga ggaagcagtt cataacgaac gtctcgcacg aattgaagac ccccctgtcg
     1741 ctcattcagg gctatgccga aggattggaa ctaaacatag cctccagtga cgaaaagcgg
     1801 aacttttact gtggagtgat caaggaggag acggcgaaaa tgggcaggct cgtcaaagac
     1861 ctcctcgatc tatcgatgat ggaaacgggg aatttaacgc tatacaagag cgagttctgc
     1921 gtcgatgagc tgataaaaag ggtgacggga aagttcgaaa aggtctttct aaaaaatgga
     1981 atcgagccga agtatgcaga gggggaaaga acggttgtct acgccgattc tttcaggacg
     2041 gagcagatac tcaccaacta cctgaacaac gccgtttacc atgtcgatga aagaaaggaa
     2101 atttttgtcc gaaaagagat tcaagagaat tcggtgattg tgcgggtgtt caactccggc
     2161 cggaacatac ccgaggaaga gagcgagaag atctggacca gtttttatag aaccgacagt
     2221 gccagaacaa gggaagagga gaggtacggc atagggcttt cgatagtcaa ggccatctgc
     2281 gatctggacg gcaaaagctg tggttttgtg aatcttccag atggcgtgga gtttttcttc
     2341 gagcttgaaa aggcgaagac ttcggattag ctcaatccaa aaaagctgaa gatagcgaat
     2401 agagacgccg acacacagcc ggcgatgatc ggggtttgca gccagcccag caagatcctc
     2461 cggatcagac ggccgtcgaa gtttctcgaa cccctggcca gtcccacacc tataacggct
     2521 ccgactatag cttgagatac ggagactgga actcctatgt aagcgtaaat gagaacggtg
     2581 agactctgcg ccaggaccgc cacgaccgcg gaaaaggcct ccagggagac tatcctcttg
     2641 cccaccgtgt acatgacttt cttgctgaag attatcgcgc cggcggcgat gctcagcccg
     2701 cctatcagcg cggcttcgtc gatcgatatg cttccgacga aagtaccggt tatattggcc
     2761 acgttgttgg cccccagagc aaaggagccg agaattccga tcacgatggc gagagccttt
     2821 agcgttatat cttgaaccac tatcgatttt atcctgcgaa agaacctcgc gatgattctg
     2881 tacaacacat atccgagcaa ggccgcgccc agaggagtcg ttatccagca gatgacgatc
     2941 ttggctacca cgaaccagtc gacgcttccg ttgagaaggc cgatacccat gatcgcgccc
     3001 actacggcct gagaggaaga taccggcagg ccgaagtgat tcaggattat caccgacaaa
     3061 cccgcggcgg ccgccacgat cgccgccgaa acgagagtct gactgctcaa actcgccaga
     3121 gtctccagtc cgttcctgcc gccgattatc gccccggcga ttattgaaag actcgcggtt
     3181 atcgccgcaa atctgtaatt caggagtccg ttggtaacag tcgtcccgta tatgttggct
     3241 gcgtcgtttc ttccgagtgc ccagccgaag aaaaccgcgg gtaaaaaggc taaaatcaat
     3301 tctatccctt catccagagt aatgaatagt attatagcac cacgctcata atattgggaa
     3361 accctttaat agaacaaaaa aacactccgg aaacccgtcg tttccgtgag tgcttcagcg
     3421 tgaatcgtta taatggcccg cctttcgtcg tgtcgcccgg agccggctgt ccctgaagcc
     3481 tgtgttgctt attccagttt tctgcgttat ttgtagagga ttatcaaaac ggctatgaca
     3541 ctaaagaaca gagcgagaag caaaaaaacc gtcgatgtat ccacaactct ccccccagtt
     3601 ttctataaaa caattgtaca actttacaaa ttgaatgtag aggttcttct acatgatatc
     3661 tccgaataga ggtctctatt gtagaatcta aatgaaagga cggtggtatt atgtacaacc
     3721 gctcggaaga ttcgacaaga atagtaatag gaataatagg aggaataatc tacgctgtcg
     3781 gcgttggatt gataatattc agtttcgcca gtttctttct cgcgcccgca atcgggacgg
     3841 tcttcacaag catgttcact ttcgtgatac ttggaatcgt cgcctcctct atcggaaggg
     3901 ccctggtcgg aatggccagg acgaggaaat acggcgggtc tgttgatgac caaagctctc
     3961 cccggagaga ggttcaagaa gatatgactt acgactacac ctttgtatcc gatagagagg
     4021 caaaaaccga aagaaagaaa cctcttagtt actccagatg ccccgaatgc gatgccgaga
     4081 acgacgacag agcctctacc tgccagaaat gcggggcttc tctggtgggt tacaaaaagt
     4141 gctcgctctg ttataaactg aacgaaatgg agaaaaggtt ctgctccaac tgcggccacg
     4201 atttcaatct cgattgatac tgcggaaaga gccgtctttg ggaagaaccg tattcacccc
     4261 tcactatcca ctaaccacca cccaccacgg ctcttaagat ggttatcgct gtaagtcttc
     4321 atgagagcaa aaaaaggatt cctgactggc gtcaggaatc gaagggggaa ttgctacgaa
     4381 acg
//
LOCUS       DAOOLY010000218         4363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_24200_length_4363_cov_4.782266, whole genome shotgun sequence.
ACCESSION   DAOOLY010000218 DAOOLY010000000
VERSION     DAOOLY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24200_length_4363_cov_4.782266"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLD15_09760"
     CDS             complement(<1..265)
                     /locus_tag="PLD15_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPB64237.1"
                     /translation="MGNLLQSYNENLHLLTPTEERILDFMISNPEKCLECTINELSKV
                     LKVSQSMIVKAARKLGYSGFTGLKLVIAGELNILVQREKSPVLL"
     gene            complement(269..1252)
                     /locus_tag="PLD15_09765"
     CDS             complement(269..1252)
                     /locus_tag="PLD15_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPB64238.1"
                     /translation="MRGFLLAIVLFGCVISLAAPFSVGILITGEIGGNAIYELVQQGA
                     QRAAGDAIEIRIVEGGYNQSKWEPLLLSMAATGKYDLLMTFTEGMPASVAKVARAFPN
                     QKFTLLDGIVPEPLANVYSVAFKDDEMTFLAGVLAGMITSSGIGGTNPEKTVGLIAGD
                     TYPAMINTMKPAYEAGVKSIEPEATVLFGIVGSWSDPTRGRELASRQYEGMADIILVI
                     AGGSGMGSIEEAAGRGRYVITVDSNALAMKPGVILASFLKHIDLLVYETIIKAGEGSL
                     PFGSNSRVGISEGMIDYTRDDPLFIKYVPEEIREKMFQWYESIKNGGLPAF"
     gene            complement(1347..2399)
                     /locus_tag="PLD15_09770"
     CDS             complement(1347..2399)
                     /locus_tag="PLD15_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64239.1"
                     /translation="MNLISRGLFFLFLIVFLSVGLVASDPADTYSATVTAYFEDIARE
                     ILELNSFEELLKLEGFFIDGPQEQPVETVKQDPASVPATTPVASRVVSLLEPVRTFAA
                     TETVYVEYDGGPLPGGVEFRFEPGDPGMGLPDVIEFYKDGVLIDTLSGKTILSPIIAL
                     DTPTVECETAVFLFLTGDYATPMGAFYLTQTDRAIYTLTVAVDPGQVRNVVDIDGDGT
                     KELRVLDDHMVKSLEVTELSEFTRFFKWTSSGWIVDKPGDFPGEYAALSLQNLEDVSV
                     KSPRTPFVWSFAYHSYMAGVELAVIRETVSELVGEYSLDWLIEPVELVKAIDLNISDY
                     AGYVMRKPSDSIIPNM"
     gene            2688..3584
                     /gene="rsgA"
                     /locus_tag="PLD15_09775"
     CDS             2688..3584
                     /gene="rsgA"
                     /locus_tag="PLD15_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPB64240.1"
                     /translation="MKGEVVNVQKGMYHVKSEDGDFFRCLLRGKLLKLEREEKSILAV
                     GDEVLFERLQKDQGIITAIEERRSSLVRKGAGKKGRHLEQVVAANIDQLLVVVAVKDP
                     PYKKSLIERYITSARSSGLDVIIVFNKADLGFSERTLEDSKVYGELGYTVKFTSVLTG
                     KGIDELAGLLSGKSTVLAGSSGVGKSSLVNAMLNGDSAKTGEVSQSTHKGKHTTTSSS
                     VYYLPGGGKLIDVPGMREFAVSDTEGLDEAFNDIAELSRGCRFNDCTHTVEPGCAVLK
                     AVEEGTLEKRRLRNYLRIKQYE"
     gene            3716..3958
                     /locus_tag="PLD15_09780"
     CDS             3716..3958
                     /locus_tag="PLD15_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64241.1"
                     /translation="MTIDQAMSFWQKGVIEPTVLHYELTTSNQWVRIGFGAPPPEKDL
                     VIYVDNSDLGWVKTDWDGRNDYCYLKVLYVYRTNTY"
     gene            3985..>4363
                     /locus_tag="PLD15_09785"
     CDS             3985..>4363
                     /locus_tag="PLD15_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64242.1"
                     /translation="MAEAYRFFFTVIIVVREAAMEKSLPILEHDYERPAVIEPAKVIP
                     PIPDMPERAIVIFYQEVIDSLNERGLLKKIVNRRSEVGLFPVYGIEHKGERVALLNPG
                     LGAPSAAGFYDELIALGVRKTVAC"
BASE COUNT         1067 a         1151 c         1155 g          990 t
ORIGIN      
        1 tcagcaggac gggcgacttt tcgcgctgga cgagaatgtt caactccccg gcgatgacga
       61 gtttaagccc ggtgaaaccg gagtatccga gttttcgggc ggctttgact atcatggact
      121 gggaaacttt cagaactttc gaaagctcgt ttatcgtaca ctccaggcac ttctccgggt
      181 tggatatcat aaagtcgagt atcctctcct cggtcggcgt caagagatgg agattttcgt
      241 tgtaggactg caacaggttt cccatgtctc aaaaggccgg cagcccgccg tttttaatgc
      301 tttcgtacca ctggaacatc ttctctctta tctcctccgg aacgtatttt atgaacagag
      361 ggtcgtcgcg cgtgtaatcg atcatgccct cggaaatacc aaccctcgaa ttggagccga
      421 agggaagaga tccctcgccg gcctttatta tagtttcata gacgagcaga tctatgtgct
      481 tgaggaagct ggccagtatg acgcccggtt tcatcgccag agcgttggaa tctacagtga
      541 ttacgtacct gcccctgccg gcagcctctt cgatcgagcc cattccgctt ccgccggcga
      601 tcaccagtat gatgtcggcc atcccttcgt actgtctcga tgccagctcc cttcccctgg
      661 tcgggtccga ccagctgccc acgatgccga agagtacggt ggcttcgggc tctatgcttt
      721 ttacgccggc ttcgtacgcc ggtttcatcg tgtttatcat cgccggatac gtatcgcccg
      781 cgatcaaccc cacggtcttt tcaggattgg ttcccccgat acccgaggag gttatcatcc
      841 cggccaggac accggccagg aaggtcattt cgtcatcctt gaaggccacg gagtaaacgt
      901 tcgccaaagg ctcgggcaca atgccgtcga gaagagtgaa cttctggttc gggaaggccc
      961 gggcgacctt tgcgacactg gcgggcatcc cctcggtgaa agtcatcagc aggtcgtatt
     1021 tccccgtagc agccatgctt agcaggagcg gctcccactt cgactgattg taaccgcctt
     1081 cgacgattct tatttctatg gcatcgccgg ccgctctctg cgctccctgt tgaaccagtt
     1141 cgtatatggc gtttccgcct atctcgcctg tgatcaaaat ccctacgctg aaaggcgccg
     1201 ccagggatat aacgcaaccg aacaacacaa tcgccagaag aaaacctctc atactatcac
     1261 tcccgaaaat caagataata cgagtatata gtatgagaac tattttctca agcatgagaa
     1321 ttttattctg agaacggggt attggtctac atgttgggga ttatactatc tgagggtttc
     1381 ctcataacgt atcccgcata atcgcttatg ttcaggtcaa ttgctttcac aagctccacg
     1441 ggctctatca gccagtcaag cgagtattcc ccgaccagct cgctgaccgt ttcccgaatc
     1501 accgccagtt ccactccggc catatacgag tggtaagcga aagaccagac gaaaggcgtt
     1561 cgggggctct tcacggagac atcctcgagg ttctgcagag agagtgccgc atactcgccc
     1621 ggaaaatccc ccggcttatc tacgatccat ccgctggaag tccatttgaa gaagcgcgtg
     1681 aactccgaca gctcggttac ctccagcgat ttgaccatat gatcgtccag cacgcggagc
     1741 tctttggttc cgtcgccgtc gatatccact acgtttctca cctggcccgg gtcgaccgca
     1801 acggtgagcg tatagatggc cctgtcggtt tgagtgagat agaaggcccc catgggagtt
     1861 gcgtaatcgc ccgtgagaaa gaggaacacg gcggtctcgc attcaaccgt cggcgtgtcg
     1921 agggctatga tcgggctgag tatagtcttt cccgaaagtg tgtcgatcaa caccccgtct
     1981 ttatagaatt cgatcacatc gggaaggccc attcccgggt ctccaggctc gaagcggaat
     2041 tccacccctc cggggagagg gcctccgtcg tactcgacgt acaccgtttc ggtggccgcg
     2101 aacgtgcgaa ccggctcaag aagagacaca accctggagg cgaccggtgt tgtggccgga
     2161 acggacgcgg gatcttgctt tacagtttcg accggctgct cctgcggccc gtcgatgaaa
     2221 aagccttcga gcttcagcag ctcttcgaag gaattcagtt ccagtatctc tctggctata
     2281 tcttcgaaat acgcggttac ggtagcagaa tatgtgtcgg cgggatcgct ggccaccaga
     2341 ccgacagata aaaagacaat taaaaataga aaaaataacc ctctagaaat caaattcatc
     2401 tcaacctcac ccgattatac acatcaatta tatactcaaa acggccgctt ggaaaaacgg
     2461 aggaacttgt tgtataataa ctcgatctgt cgaaaggagg tggtgcagaa tgaaggataa
     2521 ttttacgatg cttcttaacc ccacgggttc cggcaccgcc atccagacgg ctcgcataag
     2581 ctgacggcac caacagtccg attattgtga ccacaggcgt tccggagcct gtggtctttg
     2641 tttaattcag tcttttcaaa atcgaaaaca ataatcggag gtcatcgatg aaaggtgaag
     2701 tagtaaacgt tcaaaaggga atgtatcacg taaagtccga agacggcgat tttttccgct
     2761 gtcttcttag aggaaagctc ctaaaactgg aaagggaaga aaagagcatt ctcgccgtcg
     2821 gagacgaggt gcttttcgaa agacttcaaa aagatcaggg catcatcacc gcgatagagg
     2881 agagacgcag cagcctcgtt cgaaagggcg ccggaaagaa gggaaggcac ctcgaacagg
     2941 tggtcgccgc caacatagac cagctccttg tagtggttgc cgtcaaggat cccccctaca
     3001 aaaaaagcct catagagcgg tacatcactt cggcgaggag ttccggtctg gatgtgataa
     3061 tagtcttcaa caaggccgat ctgggatttt ccgaaagaac gctcgaagat tcgaaggttt
     3121 acggagaact cgggtacacc gtgaaattca cgagcgtgct tacaggaaag ggtatagatg
     3181 aactcgcagg cctgctgagc gggaaatcga ccgtactggc cggtagttcc ggcgtcggca
     3241 aatcctctct cgtcaacgcc atgctcaacg gcgattccgc aaaaacaggc gaggtgagcc
     3301 agtccaccca caaagggaag cacacgacca cctcttcgag tgtttactat ctgcccggcg
     3361 gaggaaagct gatagacgtg cccgggatga gagagttcgc cgtgagcgat acggaaggac
     3421 tcgacgaagc cttcaacgac atagccgagc tctccagggg ctgccgcttc aacgattgca
     3481 cccacacggt tgagccgggc tgcgcggtcc tcaaagccgt cgaagaggga acgctagaga
     3541 aaagaagact gagaaactac ctgaggatca aacaatacga atgaggcccg gacatccggg
     3601 ccttttcttc tggcggaggc accgctcgct ctttcggtcg ccgcgtttgt atttttccgt
     3661 tgctctgaaa aacactgtac agatgatggc cgttgtttga attgaaattt gggtaatgac
     3721 tatcgatcag gccatgagct tctggcaaaa aggagtaatc gagccgacag ttttgcatta
     3781 cgagctgacg acatccaacc agtgggtaag aataggtttt ggcgcgccgc ctccggagaa
     3841 agacctggtt atctacgttg acaatagcga cttaggctgg gtgaagactg attgggatgg
     3901 caggaacgac tactgctacc tcaaagtatt gtatgtatat cgcactaata cctactgatg
     3961 ctagatcggt ttcaaaggcg tcttttggcc gaagcatatc gtttcttctt cactgttata
     4021 atcgtcgtaa gggaggcagc catggagaag agtttaccca ttctagagca tgattatgaa
     4081 aggcccgcag tcatagaacc ggcgaaggtc atcccgccga tccccgatat gcccgaaagg
     4141 gcgatcgtta tcttctatca ggaggtaatc gacagcctga acgagagagg gcttctcaag
     4201 aagatcgtga acagaagaag cgaagtggga ctatttccgg tctacgggat cgaacacaaa
     4261 ggcgagcggg tggccctctt gaatccgggg ctcggggctc cctccgcggc gggcttttat
     4321 gacgagctta tcgcgctcgg tgtcaggaag acggtggcct gcg
//
LOCUS       DAOOLY010000219         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_24685_length_4299_cov_4.245759, whole genome shotgun sequence.
ACCESSION   DAOOLY010000219 DAOOLY010000000
VERSION     DAOOLY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24685_length_4299_cov_4.245759"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..791
                     /locus_tag="PLD15_09790"
                     /pseudo
     CDS             74..791
                     /locus_tag="PLD15_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            791..1684
                     /locus_tag="PLD15_09795"
     CDS             791..1684
                     /locus_tag="PLD15_09795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPB64243.1"
                     /translation="MEVIIAFGLSLVFNYIFKILGTKLGFVDKPAGALKPHEKPIPYL
                     GGTAILLSISPWFLNYPEYFFPFIVMWTVGFVDDKRGLSPKVRLFMELAGGFSISYII
                     FGYSFFDSTILAFVFAVAVNAYNMVDGLDGICAGNTIIFAIFAFIAGFSPDISLAIAG
                     AFAGYLMYNFPPAKIFMGDQGSYLAGTFVGLLLIQSWESQNFVRIAAFCWPVVLDLFI
                     GFARRSLAGKSPLKGDRDHYYDKIFAITGKKKRATFFITSSIALFYALVGFLLPVTAI
                     PFVLMASSVVQVFLLKSLRST"
     gene            complement(1677..2609)
                     /locus_tag="PLD15_09800"
     CDS             complement(1677..2609)
                     /locus_tag="PLD15_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPB64244.1"
                     /translation="MREILVSEYGKLKSIDTPIEGCWINITSPNSEDISFLKEMGFEE
                     DFVYDSLDQEERARFEQDEDLVYIITKLPYFDSEDKAVPYKTMTLGIAMKSGYFVTVS
                     SFKSAILEDLIEGRVRDVSTKKRNRFLLRIFDRGTVYYLRYLKEIRRLSNEIESELHR
                     STRNKELVAMLNLEKSLVFFTTSLRSNELMYEKLKRAHILTLYEEDEELFEDISIENR
                     QAIEMANIYSDILTGMMDAFASVISNNLNVVMKILTIVTLTLQIPTLIASIYGMNVSL
                     PFMDSPSIFYWSMGLSGLAAIIVGFILFKIRWFK"
     gene            2675..2803
                     /locus_tag="PLD15_09805"
     CDS             2675..2803
                     /locus_tag="PLD15_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64245.1"
                     /translation="MDEKDQGGGSLSLLYNSMYLTSSFRIIARRAAVDLEKFEKHI"
     gene            2840..3952
                     /locus_tag="PLD15_09810"
     CDS             2840..3952
                     /locus_tag="PLD15_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB64246.1"
                     /translation="MLHYFRPDRLQLILKELKELMKVRKFLVVLISMIACLTFGVYLS
                     DVTGEQITITWFTEEPSDSYLIVYDGSNEWIHSETEVTLHRFRVSGLRPGTSYAYIVK
                     SMRGDSLVYRGGGVLTTSPQRNSPFRFAAYGDSRSDLEVHKAVTAAIAAENASLVINT
                     GDLVSSDDDLESWKDFMQAVENLSSGVYYSAIGNHESDAINYLRFFAYPGNERYYSFW
                     YGDIFFVCLNTNESFDKYSQQYTWLLTELEKAAVLDPAFTIVFFHHPPYSFGPHGDHF
                     YVKEYLVPVFESYGVDIVLNGHDHGYQRIERNGVNYIITAGGGAPLYEILPGPGLKAY
                     GQSYHYVLFEYTLDGISIQAKTPQGEIIDSLFVRRR"
BASE COUNT         1021 a         1063 c         1079 g         1136 t
ORIGIN      
        1 cgagactgaa aagctgtctc taaaaggttt cctgcccgat ctcttcatgg gtgtcgatat
       61 tttcggccac ccgatggact gggaggggat cgaagcgatc tgccagaagt acgcgatccc
      121 gatagtcgag gattcctgcg aggctctggg cagcggttat ctcggtagga gggccgggtc
      181 cttcggactt gccggcgcct tctttttacc ccaacaaaca gataactacc ggtgagggcg
      241 gcatcatcgt aacggacgat ccgtctatag ccgagctttc caggagcatg agaaaccagg
      301 gccgtggcgt cgctgaagac tggctggaac acgttaggct tggctacaac tatcgtctgg
      361 acgagctttc tgccgccctg ggcgtcgaac agatgaaaag gatcgacgaa atactccaga
      421 agcgcagagt cgtggccgac cgctactccg ccttgctctc gaagatcgga ggagttaccg
      481 ttccttacct cgaagattac gtgacttcaa tggggtggtt tgtgtacgtg ataaggcttg
      541 atgagcggat cgacaggggg cggtttatgg atcagctgag agaaaacggg gtgcagtgtc
      601 gcgagtactt cagaccggtt catctccagc ctttctacat agaggagttc ggttaccgcg
      661 agggaagctt tcctgtgacg gagagagttt caaggcaaac tgtggccatt cccttcttca
      721 gcaacctctc tcccgatgaa caggagtacg tcgcacaggt cgtagcgaag accgtcgaaa
      781 gggtcggata atggaagtaa taatagcctt cggtctgtct ctggttttca actatatctt
      841 caagatactc ggaacgaagc tgggtttcgt cgataaacca gccggagcgc tcaaacctca
      901 cgaaaagcct ataccttatc tcggtggaac ggccatactt ctgtcgatct caccatggtt
      961 cctcaactat cccgagtact tctttccttt catcgttatg tggacggtag gttttgttga
     1021 cgacaaaagg ggactctcgc ccaaagtgag gcttttcatg gagctggccg gtggcttttc
     1081 gatatcctac ataatcttcg gttattcctt cttcgattcg accatactcg ccttcgtttt
     1141 cgctgtagcg gtcaacgctt acaacatggt ggacggtctt gatggcatct gtgcgggaaa
     1201 cacgatcatc ttcgcgatct tcgccttcat tgcgggattt tctcccgaca tatcgctggc
     1261 tatcgccggc gcttttgccg gttatctaat gtacaacttt cctcccgcga agattttcat
     1321 gggagatcag gggtcgtatc ttgccggaac tttcgtggga ttgcttctaa tacagagctg
     1381 ggaaagccag aatttcgtca ggatcgccgc cttctgctgg ccggtcgtgc tggatctctt
     1441 cataggtttc gccagacgtt cgctggcagg aaaatcgccc ctgaaaggag acagagacca
     1501 ttattacgac aagatctttg cgatcaccgg caaaaaaaag agggctacgt tttttataac
     1561 ctcttcgata gccctctttt atgctttggt aggctttctt ctaccggtaa ccgcgatccc
     1621 cttcgttctc atggcctcat ctgttgtgca ggtttttctg ctcaaatcgc tacgctctac
     1681 ttgaaccacc ttatcttgaa aaggatgaac ccgacgataa tggctgccag accggaaagc
     1741 cccatcgacc agtagaagat gctcggactg tccatgaagg gaagactcac attcattccg
     1801 tagatcgagg cgatgagcgt cggtatctgc agcgtgagag taacgatcgt gagtatcttc
     1861 atcactacgt tcaggttgtt cgatataacc gaagcgaagg cgtccatcat accggtcagt
     1921 atgtcgctgt agatgtttgc catctcgatg gcttgcctgt tttcgatgga tatgtcctcg
     1981 aagagttcct cgtcttcttc gtaaagggta agtatgtggg ccctttttag cttctcgtac
     2041 atcagttcat tggatctcag agatgtcgtg aagaagacca gagatttttc gaggttcagc
     2101 atggccacca gctccttgtt ccttgtagag cggtggagct ccgactctat ctcgtttgaa
     2161 agccgcctga tctctttgag atatctcagg taataaaccg ttcccctgtc gaagattctc
     2221 agcaggaatc tgttgcgttt ttttgtggaa acgtccctga cgcgcccttc gataagatct
     2281 tcgaggatgg cgcttttaaa actactgacg gtgacgaagt agcctgactt catggctata
     2341 ccgagagtca tagtcttgta cggaacggct ttgtcttcac tgtcgaagta ggggagtttg
     2401 gtgatgatat atacgaggtc ctcgtcctgc tcgaacctcg ctctctcttc ctggtcgaga
     2461 gagtcgtaca caaagtcctc ttcaaaaccc atttccttga gaaaggatat atcttcgctg
     2521 ttcggagaag tgatatttat ccagcagcct tctatgggtg tatcgataga cttcaactta
     2581 ccgtattcag ataccagtat ctctcgcaca ttgtttacct ccttcttgtt atcaagtcaa
     2641 ggattggttt ccccgtcccg agaaggaggt ctcaatggac gagaaagatc aaggtggcgg
     2701 ttcactctcg ctactgtata attccatgta tctcacctcc agcttcagaa ttatagcacg
     2761 tagagcagcg gtggaccttg agaagtttga aaaacacatc tgaagtattc cttgtttaag
     2821 cgtttcctct accgtccagg tgcttcacta cttcaggccg gacaggttac aattgatctt
     2881 gaaggagttg aaggagttga tgaaagtgag gaaattcttg gttgtgttga tttcgatgat
     2941 cgcctgtctt acttttggcg tttatctcag cgatgtcacc ggcgagcaga tcactattac
     3001 atggttcacc gaggaacctt ccgattccta cctgatcgtc tatgacggat cgaacgagtg
     3061 gatacattcc gagactgaag taacgctcca cagattccgt gtttccggcc tgagaccggg
     3121 cacgagttat gcgtacatcg tgaagagcat gcggggagac agcctcgttt acagaggcgg
     3181 tggagtgcta acgacctcgc ctcagaggaa ctctcccttt agattcgcag cttacggcga
     3241 cagcaggagc gatctcgagg tccataaagc cgtaacagcg gccattgcag cagagaacgc
     3301 ctctctggtg ataaacacag gagacctggt ttccagcgat gatgatctgg aaagctggaa
     3361 agacttcatg caggccgttg agaacctctc cagcggcgtt tactacagtg ccatcggcaa
     3421 ccacgaaagc gatgcgatca attaccttag attcttcgca tatccgggaa acgaacgcta
     3481 ctacagtttc tggtacggtg atatcttttt cgtctgtttg aacacgaatg aatccttcga
     3541 taaatactca caacagtata cctggttact cacagagctc gagaaggcgg ccgttctcga
     3601 tcctgccttc acaatcgtct tcttccacca ccctccttac agttttgggc cacacggcga
     3661 tcacttttat gtgaaagagt acctggtgcc ggtctttgaa agctacggcg tcgatatagt
     3721 tctgaacggc cacgatcacg gctaccagag gatagagaga aacggagtga actatataat
     3781 aacggccggc ggtggcgctc ccctctacga gatcctgccc gggccggggt tgaaggctta
     3841 cggccagtca taccactatg ttctgttcga atacaccctt gacggtattt ccatccaggc
     3901 taaaacgccg caaggagaga tcatagacag cctcttcgtc aggagacgtt aaccgagtgt
     3961 tttgacagct caacccgatt aacattatta acgccggtat aatttacact accttgatcg
     4021 acaaaaccca ccaccgatgg gagtaacgcg ggtaatattc aggcaaactt cccgcgataa
     4081 cgggaagcgg caaaaggctt tttcctactg accgcttcta aagccggttt tgcgaaggat
     4141 ccggtcatta atactcgact aaaactcgaa taacgctccc ggagtataaa cgtattgtag
     4201 aaaaacttcg ggaggtgcaa aagatgaaaa agacagttat agctattgct ctggcgactc
     4261 tttcggtggt actggcattc ggttcaacac tggttataa
//
LOCUS       DAOOLY010000220         4258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_25011_length_4258_cov_3.934095, whole genome shotgun sequence.
ACCESSION   DAOOLY010000220 DAOOLY010000000
VERSION     DAOOLY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25011_length_4258_cov_3.934095"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLD15_09815"
     CDS             complement(<1..953)
                     /locus_tag="PLD15_09815"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HPB64247.1"
                     /translation="MCGIVGMVGRDLTIKKLVDALRKLEYRGYDSAGIAFRTNEGLRV
                     EKAAGKIDVLESHLGNMMNTDVLQGMAHTRWATHGGPSDFNAHPHTDCTGKIAVVHNG
                     IIENFDELRTGLESNGHIFKSSTDTEVIAHLVEDHYRGDLVTALRHTLVDLEGAYAIG
                     VMHQDHPDIIVAARKGSPLVIGATQGGGFMASDVTPLLKYLRDVHFMEDGELAVIKSG
                     NVEILKLDGTPKVKNPTRITWSEDAAEKGGYPHFMLKEIYEEPQTLRNAITGRIKNKL
                     PHFRELEKMGEDLTKIKGVTVLACGTSYHAGLVFQRFVQDYA"
     gene            complement(959..1954)
                     /gene="plsX"
                     /locus_tag="PLD15_09820"
     CDS             complement(959..1954)
                     /gene="plsX"
                     /locus_tag="PLD15_09820"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPB64248.1"
                     /translation="MPGVRIALDSAGGDRAPKVNLEGALLALKEFPDLEILLVGRQEE
                     LSPGLKGYPEASRIKIVDAREVFPMSEKPSLLLRKKETSLYITALQVKEGGADAMVSA
                     GNTGGVLVAALFVVGRIKGVERGAIAGKIPSKNGYTILIDAGANAEVRAEHLRDFGIM
                     GYEYARFLRREPVKVGLLNVGEEEEKGTELTRMAFNYLKSELGDSFVGNVEGRDINFG
                     EVDVIVSGGFEGNVALKAIEGAGKLVSQRLREEIKKSGIAGLLGALLLKKALGRLRNS
                     MNPSEYGGAFILGVRGVVSKAHGNSDSLAIKNAIRVAYEGVKGELVEKIGKRFGG"
     gene            complement(1958..2140)
                     /gene="rpmF"
                     /locus_tag="PLD15_09825"
     CDS             complement(1958..2140)
                     /gene="rpmF"
                     /locus_tag="PLD15_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB64249.1"
                     /translation="MAVPKQKRSRSRTHLKRAKTYRAITVSVSTCPNCGEPKQPHRVC
                     LHCGHYGGRQILEIGE"
     gene            complement(2158..2694)
                     /locus_tag="PLD15_09830"
     CDS             complement(2158..2694)
                     /locus_tag="PLD15_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPB64250.1"
                     /translation="MNYRRDLVIDLNTFEFKKAIDASFGTDFIDEVTLLEPVNVHIDL
                     YKDKDSVIVTGSVSTAVEESCARCLKPVVIQIKGTIEATYVHEIMLKSVDDAGKDELE
                     NVIRLEGELLDLSDRVIEAIIVEVPLKTLCSKDCAGLCQVCGTNLNENPDHKCQDRAI
                     VDDSWHKAISKLKTKLKS"
     gene            complement(2756..3445)
                     /locus_tag="PLD15_09835"
     CDS             complement(2756..3445)
                     /locus_tag="PLD15_09835"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64251.1"
                     /translation="MLLKIDGLTKRFDGETVINNLNYSTEGKFFLTILGSSGCGKTTL
                     LRILGGVLKPDEGTVHISSERLAFVFQDDRLIPWITAGENIDIVSPGCDVNRYLRLVG
                     LEGHGGKYPSQMSGGMKRRLNIARAIAFEPDLILMDEPFNSLDVVMKDRLLEEIKEIW
                     HERKLSIVMVTHDPREAARLSTEIMLVRDGFGKTEIVKLENPMTRSADDIDTISRRLL
                     ERMKEFSSFVQ"
     gene            complement(3429..4190)
                     /locus_tag="PLD15_09840"
     CDS             complement(3429..4190)
                     /locus_tag="PLD15_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64252.1"
                     /translation="MGSSSKKKVISPLLSIGILILVWFVAAEIVSSNLLLPGPAETFE
                     GLWKIVTSIDGWETILETCAKAFAGLFIALGMALVSGFVMGLVEIVYELLKPVMTVLQ
                     SIPIVSWLALAIFWWGVGFKSPVYIVFLTLFPIFTINIAEGVRNVDKKLVEMAKIYRL
                     PGRVVLTKIYFSSAFPFIVSSMRVGIGIMWKSVAVAEFMVGTSGIGRVIADSKYSINI
                     VRVFAYTLVLVVLGIVTEKALDFMIRKGNRYALKN"
BASE COUNT          974 a         1201 c          923 g         1160 t
ORIGIN      
        1 gcgtaatcct gaacgaaacg ctggaaaaca agcccggcat ggtagctggt tccgcaggct
       61 agcacggtta ctcccttaat tttcgttaaa tcctcgccca ttttttcaag ctctctgaaa
      121 tgcggcagtt tgttctttat tcttccggtg atagcatttc tgagtgtctg gggttcttcg
      181 tatatctctt tcaacatgaa gtgaggataa ccgcccttct cggccgcgtc ttcgctccag
      241 gtgatcctcg tcgggttctt gactttggga gtaccgtcca atttcaagat ctccacgttt
      301 cccgatttta ttacggcgag ctccccgtct tccatgaaat gcacatcccg cagatacttg
      361 agcaaaggag tgacatccga agccatgaac cctccgccct gtgttgcacc tatgacgaga
      421 gggctaccct ttctggccgc gactattata tcgggatggt cctgatgcat cacgcctatc
      481 gcgtacgcgc cctcaaggtc aactagagtg tgcctcaagg cggtgaccag gtctcccctg
      541 taatggtcct caacgagatg ggctatcacc tcggtgtccg tggaggattt gaatatatgg
      601 ccgttgctct ccaggccggt tctgagctca tcgaagttct cgattatgcc gttgtgaacg
      661 acggcgatct ttccagtaca gtccgtgtgt ggatgtgcgt tgaagtccga aggccctccg
      721 tgggtcgccc acctggtgtg agccatgccc tgaagaacgt cggtgttcat catattcccg
      781 agatgagact cgagaacatc tatcttcccg gcagcctttt ccactctcag accctcgttt
      841 gttctgaagg ctatgccggc cgagtcgtaa ccccggtact caagcttcct gagcgcatca
      901 acgagtttct tgatcgttaa atcgcgtccc accattccca ctattccaca caaaccactt
      961 accccccaaa cctctttcca attttctcca caagctcgcc cttgacacct tcgtacgcaa
     1021 ctctaatggc gttctttatg gcgagagagt cggaatttcc gtgggctttg gagaccactc
     1081 ccctgacacc cagtatgaaa gccccgccgt actcgctggg attcatggag tttctgagcc
     1141 ttcccagggc cttcttcaat agcagggcac ctagaagacc ggctattcca ctctttttta
     1201 tctcttctct tagtctttga gagacgagct ttccagcccc ttcaatggcc ttcagggcaa
     1261 cgttgccttc gaagcctccg ctgactatga cgtcaacctc gccgaagttt atatctcttc
     1321 cctcgacgtt tccgacaaag ctgtctccca gttccgactt caagtaatta aaggccattc
     1381 tggtcagttc ggtgcccttc tcttcctcct cgccgacgtt gaggagcccg acttttaccg
     1441 gctctcttct cagaaatctg gcgtactcgt aacccataat tccgaaatcc ctgaggtgtt
     1501 cggctcttac ttcggcgttc gcgccggcat ctatgaggat cgtatagccg tttttagaag
     1561 gtatttttcc ggcgatcgcg cctctttcga cacctttgat cctgcctaca acgaaaagcg
     1621 ccgcgacgag tacgccacct gtgtttccgg ccgataccat cgcgtcggct ccgccttcct
     1681 tgacctgcag ggccgttata taaagagaag tctctttctt tctcaacaaa agagagggct
     1741 tttcagacat tggaaagacc tctctcgcat cgactatttt tattcttgaa gcctctggat
     1801 atcctttcaa accgggggaa agttcctctt gcctgccgac cagaagtatt tccagatcgg
     1861 gaaactcttt cagagccaga agagcacctt caagattcac tttcggtgcc ctgtctcctc
     1921 cagcagaatc gagcgcgatt ctcacgccgg gcatggatta ctcaccaatc tcaagaatct
     1981 gtcttccccc gtaatgacca cagtgcagac aaaccctgtg aggttgcttg ggttctccgc
     2041 agttaggaca cgtagaaaca gagacggtga tcgccctgta cgtcttcgct ctcttgagat
     2101 gagttctgct tctacttcgt ttttgctttg gaacggccac tttaatccct cctgtattta
     2161 actcttcaat ttggttttca atttggagat cgctttgtgc cagctatcat caacaatcgc
     2221 ccgatcctgg cacttgtgat cgggattctc gttcagattg gttccacata cctggcacaa
     2281 accggcacag tccttgctgc atagagtctt cagcggaacc tcaacaatta tagcctcaat
     2341 aactcgatct gacaagtcca gaagctctcc ctctaatctt ataacgttct ccagctcgtc
     2401 tttgccggca tcatcgacgc tcttcagcat tatctcgtga acgtaagtcg cttcgattgt
     2461 tccctttatc tgtataacca ccggcttcag gcagcgggca caactttctt cgaccgcggt
     2521 actgacgcta ccggtaacta tgaccgaatc tttgtctttg tagagatcta tatggacgtt
     2581 gaccggttcc agcagagtca cttcatcaat gaagtctgtc ccgaacgaag catctatcgc
     2641 ctttttgaac tcgaacgtat ttaggtctat caccagatct cttctatagt tcacaataat
     2701 cacctctttg ctgccagcaa atactacatc aaggttgttt cgattcattc gctttttatt
     2761 gaacaaaaga agaaaattct ttcattctct ccagcagtct tctcgatatg gtatcgatgt
     2821 cgtcggcgct tctggtcatc gggttttcca gcttaactat ttcagttttg ccgaagccat
     2881 cgcgaaccag cattatctca gtcgacagtc tcgccgcctc tctcgggtcg tgtgtaacca
     2941 ttactatact gagtttcctt tcgtgccata tctcctttat ctcttccagc aagcggtcct
     3001 tcattacgac gtccagtgag ttgaagggtt cgtccatgag tataagatcc ggctcgaagg
     3061 cgatcgccct cgctatattg agccttcgtt tcataccgcc gctcatctgt gagggatact
     3121 tccctccgtg gccctcaagg cccaccagtc tcaggtatct gttcacgtca cagccgggcg
     3181 agactatgtc gatattttct ccggcagtta tccacggtat cagccgatcg tcctgaaaga
     3241 caaaggcaag gcgctcgctg gatatatgca cagtaccctc atcaggtttg aggacaccgc
     3301 cgagaatacg gagcaaagta gtctttccac accccgaact gccgagtata gtgagaaaga
     3361 acttgccctc tgtactgtag ttgaggttat tgataaccgt ctccccatcg aacctcttcg
     3421 tgagtccgtc aatttttaag agcatagcgg ttacccttcc ttatcatgaa gtcgagagcc
     3481 ttttccgtga ctatgcccag tacaaccagc acaagcgtgt aggcgaaaac cctcacgata
     3541 tttatagagt acttggaatc ggcaatgact cttccgatcc cggacgttcc aaccataaac
     3601 tcggcaacgg caacggactt ccacatgata cctatgccaa ccctcatcga agagactatg
     3661 aaaggaaaag ccgaagagaa gtagatcttt gtaagcacga cccttccggg caatctgtag
     3721 atcttcgcca tttcgaccag tttcttgtca acgttcctta caccctctgc gatgtttatg
     3781 gtaaatatgg ggaacagtgt caggaagacg atataaaccg gtgatttgaa ccccactccc
     3841 caccagaata tggccagtgc aagccaggaa actatgggga tagactggag aacggtcata
     3901 acgggtttga gtagctcgta aacgatctcg acaagaccca tgacaaaacc gctgaccagc
     3961 gccattccca gggctatgaa aagcccggcg aaggctttgg cacacgtttc aagtatcgtt
     4021 tcccagccat cgatgctggt aacgattttc cagagaccct cgaaagtctc ggcgggtccg
     4081 ggaagaagaa ggttgctcga aactatctcc gccgccacaa accatacgag tatcaatata
     4141 ccaatagata atagcggaga tattaccttc tttttagaag aagaacccat ctagattcac
     4201 ttcacctgtc aactcaggat caagctcaaa aagcctttcc aggtaaatcg tgacttct
//
LOCUS       DAOOLY010000221         4254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_25046_length_4254_cov_4.262205, whole genome shotgun sequence.
ACCESSION   DAOOLY010000221 DAOOLY010000000
VERSION     DAOOLY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25046_length_4254_cov_4.262205"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2773)
                     /locus_tag="PLD15_09845"
     CDS             complement(158..2773)
                     /locus_tag="PLD15_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64253.1"
                     /translation="MLKSICFLLFPLVFVSCILAQDRPVFPIYSGAIVSSVSPVFDQT
                     IYAVISTNDKTEKVGEWYLSSLKQAGWKIEEEDVGEYNDGWSINASHKTYNLQLEMNT
                     NIAGDTLTVMIMLQGEGVHKEIFGGQTPSIGQIEDGKLVDDQGIPLDGAVDLPEELEN
                     YFALSDDLFKLLDEFCLEPSPEKAQQIVSKIETYMAASEVAGYMLGDRALSQDNSFAA
                     SAYISGSQMDFQIGFICFFLETALNEPDRFDELAEQVCKMKNGLQNDQMPRMKELARA
                     AEELSKELENYGYWNSFAEPTLMTDDFVVEVGETPPEYSTRLTATRIFTPSREFWAER
                     LRETWINLRNWNAGCPEGDQSVDTDGDGWDDTLDCKAFGLIPFEDEIRHCLGLPRTEE
                     REIEFLSDPMTVYCMVALAVEVGNYEQLLSDGELRVWVNNEPLMTVVLPADNLKRASL
                     NPWDYQIDLEIQGFSNPLGNPAEPFLNGVRLVYGDEAADYYRAAYRYPPSVSEATGGE
                     FNGFVYFLHIGEPIILLSEDPNDGVILLSGSKDDIATQYLKFMNLARKNHPALEKVNR
                     IRIQAIAGGVPGNIVEFTIPAVFSNLPEQVGTWEGWPSDRPVSEWNFGGELGETPLPD
                     VEPKIEITPGSGMVMLEENKRYAVTCTLHVLEHWIKMVQEMTIEAVVKKATSTENMPT
                     PGSNAFLKCFGETSGGKYITNAVNNLKNWYPQHASAAVSEYFSKTLLNFTGEYMGKTI
                     DIVTGKTLFQTGKQLAQQIDIMQDVTFRGLTTEAKMRISHHRYLMWKMANLANTELKL
                     WREIDKPLKEVLGVFEGIVGKEAAAYLTQPVNWFRRSVYGLDAIHHLYTIYKIQVELY
                     SIERILMDMGSWISM"
     gene            3209..3766
                     /locus_tag="PLD15_09850"
     CDS             3209..3766
                     /locus_tag="PLD15_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64254.1"
                     /translation="MADRSKKQSRTATKKNALDIINGNDALLILKALANEDRSIAKRI
                     EQIALEYLRDVDFEKIASQVYYALERIEVEDLWDQSGRMRDGYVEPSERAWEMFEEAL
                     EPFTNELKKYLDLSLDNEAKNYCMGILKGLHRFDKESTSQFKDWVEDAPDEFFERILN
                     DWKKACKKPEHIQEMEDFIERGLGN"
     gene            3967..4137
                     /locus_tag="PLD15_09855"
     CDS             3967..4137
                     /locus_tag="PLD15_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64255.1"
                     /translation="MSRMVLMLAGVIVLVMALLASLNIATVVEPVWHVVLKWVGGAIM
                     VLVAYIDKGKKV"
BASE COUNT         1084 a         1034 c          984 g         1152 t
ORIGIN      
        1 ccggtcagtc ccccattttc gaaaagaggt aagaggcatg aggttagagg ttagaaaatc
       61 aagatcttcc gctcttcaca cctctcacct ctggcctctg acctctgttt taccaaaaaa
      121 cctggacgcg cggcgtcgga actaacaacc gcccttttca cattgaaatc cagcttccca
      181 tgtccatcag gattctttct atgctgtaca gttcgacctg tatcttgtaa atggtataga
      241 ggtgatggat cgcatcgagt ccatagacgc tcctcctgaa ccagttaacc ggttgtgtga
      301 ggtaagcagc ggcctccttt cccactattc cttcgaagac accgagaact tccttcaaag
      361 gtttgtcgat ttctctccag agtttcagtt ctgtgttggc aagattggcc atcttccaca
      421 tcagataacg atggtggctt attcgcatct tcgcttccgt ggtcagccct ctgaaggtga
      481 cgtcctgcat tatatctatc tgctgggcta gttgcttgcc tgtctggaag agggtttttc
      541 cagtcactat atcgatcgtt tttcccatgt attcacctgt aaagttcagt aaggtcttgc
      601 tgaagtattc ggaaacggct gcactggcgt gttgaggata ccagttcttc aagttgttca
      661 cggcgttcgt tatgtatttg ccgccgctcg tctcgccaaa acacttgagg aaggcattgc
      721 ttcccggagt gggcatattc tcggtcgatg tcgccttctt gacaacggcc tcgattgtca
      781 tctcctgcac catttttatc cagtgttcaa gcacgtgaag tgtacaggtc accgcgtatc
      841 tcttgttctc ttcaagcatc accattccgc tgcctggagt gatttcgatt ttcggttcca
      901 catccggcag tggtgtttca cccagttccc cgccaaagtt ccattcactt acaggacgat
      961 cgcttggcca gccttcccag gtccccacct gttcgggaag gtttgaaaag accgccggta
     1021 ttgtgaattc aactatgttt ccaggcacgc ctcctgcgat cgcctggatt ctgattctgt
     1081 tgactttctc gagagcggga tgattctttc gtgcgaggtt catgaacttc aggtattgag
     1141 tcgcgatgtc gtctttgctt cccgaaagta gtattacccc atcgttcggg tcttcggaca
     1201 gcaatataat cggctctcca atatggagga agtaaacgaa gccgttgaac tccccgcccg
     1261 tcgcttcgct tacgctcgga ggataacggt aagccgccct gtaataatcg gccgcctcgt
     1321 cgccatacac aagcctgaca ccgttaagga acggttcagc gggattaccc agagggttcg
     1381 agaagccctg aatttctaga tcgatctggt aatcccacgg gtttagcgag gccctcttga
     1441 gattatccgc gggaagaact accgtcatca gcggttcgtt gtttacccat actcgaagct
     1501 ctccatcgga aagaagttgc tcgtagtttc ctacctctac ggcaagcgca accatacagt
     1561 aaaccgtcat cgggtcgcta aggaactcta tctcgcgctc ttcggtcctc ggcaatccca
     1621 ggcagtgacg aatttcatcc tcgaagggga tcagaccaaa ggccttgcaa tcgagggtat
     1681 catcccatcc atctccatcg gtatcgacac tctgatcgcc ttccggacag ccggcattcc
     1741 agtttctaag gtttatccag gtttcgcgca atctttccgc ccagaactct ctgctcggag
     1801 taaagatgcg tgtagccgta agccttgtcg agtattcggg aggcgtctcc cccacttcaa
     1861 cgacgaaatc gtcggtcatc aaagtaggct cggcgaagct gttccagtag ccgtaattct
     1921 cgagttcttt ggaaagttct tcggctgccc tggcaagttc tttcattctc ggcatctgat
     1981 cgttctgcag gccgttcttc attttgcaga cctgttcggc cagttcgtca aatctatccg
     2041 gttcgttaag ggcagtctcc agaaagaaac agataaaacc gatctgaaaa tccatctggc
     2101 tcccagatat ataagccgaa gcggcaaagg aattgtcctg agagagcgca cgatcgccaa
     2161 gcatataacc tgcaacttca cttgccgcca tgtaagtctc gatctttgac actatttgtt
     2221 gagccttctc cggagagggt tcgagacaga attcgtcaag aagtttgaag agatcgtccg
     2281 agagggcgaa gtaattctcc agttcttccg gaaggtcaac agccccatcg agaggaatac
     2341 cttgatcatc aaccagtttc ccatcctcta tttgcccgat cgaaggagtc tgcccaccaa
     2401 aaatctcttt gtgtaccccc tctccttgaa gcataatcat cacggtgaga gtgtcgcccg
     2461 ctatgttcgt attcatttct agctgaaggt tgtaagtctt atgactggcg tttatagacc
     2521 agccgtcgtt gtattcgccc acatcctctt cttcgatctt ccagccggcc tgtttaaggc
     2581 tggataggta ccattcaccg actttctccg tcttgtcgtt cgtggaaatc acggcatata
     2641 ttgtttgatc aaaaactgga gaaacgctgg atacgatcgc tcccgaatag atagggaaca
     2701 cgggcctatc ttgcgcaaga atacaactca caaaaaccag tggaaaaaga agaaaacaaa
     2761 tactcttcag catataccac acccccgaaa aagagacggg ttgaaaatcg tctggagttt
     2821 ttctcaaggt ttttggtgaa cgaatagaat atagtcaaaa catgttcgat acgattctta
     2881 ttagactatt caactcgttt agacgattat ataatggcac tgaaccaaaa cagatcgatt
     2941 tcaagcatac gatctattga cttgtgtatg gcaaaatgga agtccttatt cctcaattct
     3001 ggtctcacta ttgtccgtag attggcttct caggactgta cgatcagata ttagcatatc
     3061 tatttcattc gcccttttct gcgttgaatc ctccgcactc atcataatcc caactggaga
     3121 ccacaatcat ctagctcact gtcagagagg tataatcacg ttaacctgta ccttctgagc
     3181 gtaacgacgt gggtgttgag gtgaaacaat ggcggatagg agcaagaaac aatctcgaac
     3241 agcaactaag aagaacgctc ttgacataat caacggaaat gatgctcttc ttatactgaa
     3301 agctttggcc aacgaagatc gaagcattgc aaaacgcatt gaacagatcg cattggaata
     3361 cctgcgcgat gtagattttg agaaaattgc ttctcaagtg tactatgcgt tggaaagaat
     3421 tgaagttgag gatctctggg accagtcagg cagaatgagg gatgggtatg tggaaccatc
     3481 agaaagagcg tgggaaatgt ttgaagaggc tttagaacca ttcacaaatg agctgaaaaa
     3541 atatctggac ctttcactgg acaacgaggc gaagaattac tgcatgggta tcctgaaagg
     3601 actgcaccga ttcgacaaag aatcaacatc ccagttcaaa gactgggtcg aagatgcccc
     3661 agatgagttt tttgaaagga ttctgaatga ctggaagaag gcctgcaaaa agcctgaaca
     3721 tatacaggaa atggaagact tcatcgaacg gggtttggga aactgacagt ttggacaaag
     3781 acgaacaaag attggcaata taaaaggtgc acctatctct tgatcttgtc aacacgctaa
     3841 tgtacgtgta gtcgacccct cctttgaaaa aaagtctagt ttgactgtac ttgttataga
     3901 atcacagtag cagatggagt caagctatgc accagatacc tgaaaatagc agaaggggtg
     3961 atcgtcatgt ccagaatggt tttgatgctc gccggtgtga tagttctggt tatggctctg
     4021 ttagcttcgc tcaacatagc aacggttgta gagcccgttt ggcatgtagt gctcaagtgg
     4081 gtcggcggtg ccataatggt actcgtcgcc tacattgaca agggcaagaa agtgtagttt
     4141 aactaagact atcaaaggcc cggagagagt tctccgggcc gtttattttt ttaagacatt
     4201 tttcggtgat ttgaaaaatc agggtgtcta tcccggtatt tatcattcgc gcca
//
LOCUS       DAOOLY010000222         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_25416_length_4206_cov_4.439171, whole genome shotgun sequence.
ACCESSION   DAOOLY010000222 DAOOLY010000000
VERSION     DAOOLY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25416_length_4206_cov_4.439171"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLD15_09860"
     CDS             complement(<1..290)
                     /locus_tag="PLD15_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB64256.1"
                     /translation="MKRLSRKISLQFFIFVLAVLVVLIVLLSVIFVKAATDEYTNYLE
                     SKKQAVYRWFIDLRRDFKSYVDGYSMEGFIDNNLDLLVEKGESTVILRNRPGL"
     gene            complement(338..841)
                     /locus_tag="PLD15_09865"
     CDS             complement(338..841)
                     /locus_tag="PLD15_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64257.1"
                     /translation="MKRISIIALFMVLISNIFGVVLADDILSLDTDPTLERSISFTDF
                     QATVTVIPISALNELEIEIENLIGLMIFELPPDWSRVDFEYISVSGRCRRGQPIEDSW
                     ADLFLIGDGSIVQTVRYNPLIVVSSGRLVLALSAYIDTGSRYSLSLNSSRGSAYWEFD
                     AGQKSVH"
     gene            1218..2276
                     /locus_tag="PLD15_09870"
     CDS             1218..2276
                     /locus_tag="PLD15_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB64258.1"
                     /translation="METIKVYILDNGSSELNSVFRKISRIKGNIQITILTEEPASKNN
                     LQNDRCERANNLLKTKYEGFSNLIEDLVFLVKLDDPANPGKIVEINNRVVEKLGYSYE
                     TLLSKSFYSLMSDPDGDFGLIRADLTLKGRCNFNSSLTGSDGSMLPVEMWMKVLDLQG
                     DIVTMVVARDQSSYEKIQAELQWRLEFEKVISKVSGILKDYDDIDRAIDKALAELCRL
                     TGADRSYVFLFDHESDTMSNTHEWCREGVQSEKETLQKLQLSDYPWWMKELQRGEVMN
                     IVVDGLPEDAKPEAEVMRRQKIQSLVVIPIFARQKLVGFAGLDSLEGYFEWSGADIEL
                     LKVFVSLISEVVGYSLLT"
     gene            2595..3698
                     /locus_tag="PLD15_09875"
     CDS             2595..3698
                     /locus_tag="PLD15_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64259.1"
                     /translation="MRKLVLIVLVVFLSVSAFSTIKVGAILPMTGGVAAFGQMIWQGV
                     QLANELFPQVLGQEIEIVLLDNNSDKVQAVNTTRRAIEQDKVVAIIGQVISSNTIAGG
                     TVAEESGVAMVSPSSTNPLVTQDRKYVSRVCFSDPFQGVAAALLAFNNMGAKNVAVFV
                     DVEQDYAVGFANFFKDTAIELGGTVFYEYYKSGDQDFTAQISDALSKNADAFFIPGYY
                     QEIALIAIQARQLGFYDPIIAGDGAAVPETIEIGGAAVNGLYFTTHYDSGSPAVTDNA
                     KLFVEAYTKKYGEAPGTFTALGFDTYLVIRDAIERAGSTDREAIAQAVRQTKDFPAVT
                     GIITINESGDAIKSVAIVKIENGKFVYDTTINP"
     gene            3775..>4206
                     /locus_tag="PLD15_09880"
     CDS             3775..>4206
                     /locus_tag="PLD15_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB64260.1"
                     /translation="MDPKTLLQNFVNGLSLGSLYALIAIGYTMVYGVLRLINFAHGDI
                     FMMAAYFTFFFVTLTQLPWYFAAIFAIASAAVLGFGVDRIAYKPIRKAPRISALITAI
                     GVSFFLESLAVVIFSGIPRSFRTIFPQFLNEMIILGGHMEGK"
BASE COUNT         1114 a         1001 c          995 g         1096 t
ORIGIN      
        1 agtcccggtc tgttgcgaag gataaccgtg ctttcaccct tctcaaccag caggtctagg
       61 ttgttgtcta tgaagccctc catcgagtaa ccatcgacat aagacttgaa atcgcgtctc
      121 aagtctataa accatctgta aaccgcctgt tttttagatt ctagatagtt cgtatattca
      181 tcggtggcgg ctttgacaaa tatcaccgag agaagaacta taagaacaac cagaacagcc
      241 agaacaaaga taaaaaactg cagggatatc tttctgctta accttttcat agcccctccc
      301 gtccgaaaag aagcttctcc cccgaccctt tgaacgatca gtgaacgctc ttttgcccgg
      361 catcgaactc ccagtatgcg cttccccttg aagaattcag cgatagactg taccggctcc
      421 ctgtatctat gtaagccgat aaagctagaa caagccttcc ggagctcacc acaatgagag
      481 gattatatct gacggtctgt acgattgaac cgtcacctat caaaaagaga tcggcccacg
      541 agtcttctat aggctgtccg cgcctgcatc taccgctcac ggatatatat tcaaaatcga
      601 cccgactcca atctggcggt agttcgaaga tcatcagacc gattaagttt tcgatttcta
      661 tttccagttc attcaatgca gagatcggga tcaccgtgac tgtagcctgg aaatctgtaa
      721 aggagatgct tctctctaga gtcggatccg tatccagtga gagaatatca tcggctaaaa
      781 ccacaccgaa aatatttgag ataagcacca taaaaagagc gattatcgag attctcttca
      841 tgaatacctc cgcaaatccc gaaaggagtc gccgtaatcg cccctccggg gtcatataga
      901 ttatagtatc tttcgcatct ttctcccaat ccgcaaccga acttgtgagt ttcccgttaa
      961 gggacctcat cgttacccac gagtttgtcg tgtcagtctg ccctcgctta acgatgtctc
     1021 tggctcctaa taagaacggt tgttggttgc gcatagcagg ttgtgcagtg aaaaccacga
     1081 gaatgaaaga gcctttcgag aggttcgata cgctcttccg ctttttcgaa agacgggtcc
     1141 acagtctttg ttaaacatca acttccgggc aactttaact gtataatata ttattgattt
     1201 tttggctggg ggaagctatg gaaacgataa aggtttatat tctagataac ggttcctcgg
     1261 agttgaattc ggttttcaga aagatctcca gaatcaaagg aaacatccag atcactattt
     1321 tgacggagga gccggcaagt aaaaacaatc ttcagaacga tcgttgcgaa agggcgaaca
     1381 accttctaaa gacgaaatac gagggctttt cgaacctcat cgaagatctc gtctttcttg
     1441 tgaagctgga tgatccggcc aatccgggaa agatagtcga gataaacaac agggttgttg
     1501 aaaaattggg gtactcttat gaaactcttt tgagtaagag cttttactcg ctgatgagtg
     1561 atcccgatgg tgatttcggc ttaataaggg ccgatctgac gttaaaaggc agatgcaatt
     1621 tcaattcatc cctgacaggc agcgatggtt cgatgctgcc cgttgaaatg tggatgaagg
     1681 tactggatct tcagggggat atagtcacca tggttgtcgc cagagaccag agctcttatg
     1741 aaaagataca ggcagaatta cagtggagac tagagttcga aaaggtgata tcgaaagtat
     1801 cgggaattct caaggactac gatgatatag acagggcaat cgacaaggca ctcgccgagc
     1861 tctgccgttt gaccggagcc gacagatctt acgtatttct cttcgatcac gaatccgaca
     1921 ccatgagcaa cacgcatgaa tggtgcagag aaggcgtcca atctgagaaa gagactctcc
     1981 agaagcttca attgagcgat tatccctggt ggatgaaaga gctccagagg ggcgaggtta
     2041 tgaacatagt cgtcgatgga cttccggaag atgctaaacc cgaggcagag gttatgagaa
     2101 gacagaaaat acagtctctg gttgtgattc cgattttcgc gaggcaaaaa ctggtaggtt
     2161 tcgccggtct ggacagtctg gaaggttact tcgaatggag cggcgccgat atagagcttc
     2221 tgaaggtctt cgtgtccctc atttcagaag tcgttggtta ttcgcttttg acctgagagt
     2281 aaggaacaat ctccttaaga aaagtcaact ccctctatac cccttctgag gacattaaac
     2341 ccgattggaa agtcctatct ttgatatgtt accgaatatc tactctttgt tgcacttgtt
     2401 tacagtaaat tcttctccta aatgctaata ttccaacaat acaaaaaaac acgcaaatct
     2461 ccgcataagt gaacacagag tcattttttt gcgcttattg actttaggtg attctaaaag
     2521 cacctggcga cgcttgaaaa tcaattttgg gttagtctca tttacttgtc accacttcag
     2581 gaggaggtaa gagtatgaga aaactcgttc ttattgtact ggtcgtcttt ctttccgttt
     2641 ccgcgttctc gacaatcaaa gtaggggcta ttcttccgat gacgggtggg gtagcagcct
     2701 tcggacagat gatctggcag ggtgttcagc tagccaatga gctctttccg caggttttag
     2761 gacaggaaat cgaaattgtt cttctcgaca acaattccga caaggtccag gctgtgaaca
     2821 caacccgtcg tgccatcgaa caggataaag tagtagccat catcgggcag gtaataagct
     2881 caaacaccat cgcagggggt accgtggcag aagagagcgg cgttgcgatg gtctctccga
     2941 gttccaccaa ccctctcgtg acacaggatc gtaaatacgt ttcccgtgtt tgtttcagcg
     3001 atccatttca gggtgttgcg gccgcgctgc tggccttcaa caacatggga gctaaaaacg
     3061 tagccgtctt tgtagacgtt gagcaggact acgctgtcgg tttcgccaac ttcttcaaag
     3121 atacggcaat tgaactcggc ggaaccgtct tctacgaata ctacaagtcg ggggatcagg
     3181 atttcaccgc ccagatttcc gatgctctct cgaagaatgc ggacgccttt ttcatccccg
     3241 gttactacca ggagatagct ctaattgcca ttcaggcgcg tcagctgggt ttctacgacc
     3301 ccataatcgc cggtgacgga gctgccgttc ccgagaccat agagatcggc ggtgcggccg
     3361 taaacggtct ctatttcacc acccactacg attctggtag tccggcagtt acagataacg
     3421 caaagctctt cgtcgaagcc tacaccaaaa agtatggaga ggcaccggga acgttcaccg
     3481 cgctgggctt cgatacttac ctggtgataa gagatgccat cgaacgtgca ggaagcaccg
     3541 atcgcgaagc gatcgcccag gccgtgcgcc agaccaaaga ctttccggcc gtaaccggta
     3601 tcatcaccat caacgagtcc ggtgatgcca taaagtccgt agcgatcgtg aagatagaga
     3661 acggtaagtt cgtgtacgat acgactataa atccgtaatg gaatcggggg accgcacggt
     3721 ccccctttcc cttcgccttc ttcactcgga cagaacgcag ccggaggtgt agatttggat
     3781 ccaaagaccc ttcttcaaaa tttcgttaat ggtctcagtc tcggttcact ttatgcgctg
     3841 atagcgatcg gttatactat ggtctatggt gttttgagac tcatcaactt cgctcacggt
     3901 gacatcttta tgatggccgc gtacttcact tttttctttg taacgctcac acagttgccc
     3961 tggtattttg ccgcaatttt tgcgatagcc agtgcggccg tgctgggatt tggtgttgac
     4021 agaattgcgt acaagcccat aaggaaggcg ccgagaatat cggctttgat taccgcaatc
     4081 ggtgtttcct tctttctgga gagtctggca gtcgttatat tttccggtat tcccagatcc
     4141 ttcagaacca tcttcccgca gttcctgaac gaaatgataa ttctcggagg tcacatggaa
     4201 gggaag
//
LOCUS       DAOOLY010000223         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_25856_length_4149_cov_3.994871, whole genome shotgun sequence.
ACCESSION   DAOOLY010000223 DAOOLY010000000
VERSION     DAOOLY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25856_length_4149_cov_3.994871"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLD15_09885"
     CDS             <1..581
                     /locus_tag="PLD15_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64261.1"
                     /translation="EELNRIKTRIEKAKEYLAKSSYRGEKVRLLIYTSKEQMELAEYI
                     KLFLSKIGINVVVDPQGHEAVTAALKKADFDMSLTTYSLGFHPEMAFYHLHSSRGTMQ
                     NGQVSGFNYGGVNIPELDETLNIIWTAFDEKVQRDSFHILQEQVREFVPVVPICIPNR
                     LEAFSRKNLDGWSISETEGVLSTETLKNLKSK"
     gene            592..1554
                     /locus_tag="PLD15_09890"
     CDS             592..1554
                     /locus_tag="PLD15_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64262.1"
                     /translation="MTLSYLVKRILIMILTVFLAVTLSFILIRSLPGDPAERYYGDPR
                     LPEEIKEEIKAVFGLDKPLITQYFIFIGNAFRGDLGVSYTYRRNVVGVILERLPWTLM
                     LTVVPTVLSMFVALWIGTFIAFNRGKKFDRFMRFFAFGLNSVPTFWLALLLVMGTGFY
                     LNIFPLQGMFDPLESGMARVISILHHAALPWITLFIVGMPSFAIQVRNIALNILGEDF
                     IITAKAKGLREQEIRRKHVLRNALGPLFSLLTLRIAGLVGGAVLIETIFSWKGMGLLV
                     LEASKNSDYPLLQATVLMTIVLVILANFLADVLQAVFDPRVIYT"
     gene            1551..2408
                     /locus_tag="PLD15_09895"
     CDS             1551..2408
                     /locus_tag="PLD15_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64263.1"
                     /translation="MKKYRKVLRDIWKAPEGKVSLFILVLFVFLAVTADWISPYDPYD
                     ITQRGFPLEAPSKEYPLGTDGFGVDILSQIIHGSRVSLTIGFVTGIGIAFMGGFIGVM
                     AGTYGGLADLFSMRAVDFIMVLPGLPIMILLMTTLGSSFWVMVMIFVLFGWASVARVT
                     RAIVLSERKRGYVEAARCAGAKKSHILLRHLLPATYSILFVTAAFAAGGSILAEAGLA
                     FLGFGDPRLVSWGKMLNYARTYNAMVLGAWWWIIFPGIAVFLTSFSIMMLGVALEKTF
                     NPRLRERDR"
     gene            2409..3101
                     /locus_tag="PLD15_09900"
     CDS             2409..3101
                     /locus_tag="PLD15_09900"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB64264.1"
                     /translation="MVEYIENTAKFEFRGKSYEYRLHEMPEEFFRWQVGNRLKGLKIF
                     LGEEKGMPNFSPHTVVMNTLEYENEFCINSCIKGLGLVPRSDKIQELGERAMELIRKS
                     YEIGADKTFRERVGMLVELYSNPETFDRSVLSSMEMYKKRTYKNVLEDPRANLLFYDN
                     QTGYSVMFSVVCEFVPRGGAYYNYVSAIHDLFHLPKDPSIKTDRYDFAYRFHLVEAFE
                     KTPGPRASTRIY"
     gene            complement(3146..3853)
                     /locus_tag="PLD15_09905"
     CDS             complement(3146..3853)
                     /locus_tag="PLD15_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPB64265.1"
                     /translation="MLLPGDRGRVERIAKYFENPVHVAKNREYFTITGEISGERVSVM
                     STGMGGPCMAIAVEELRALGVHTLIRVGSAGSLQKKLKMGEGVIVTGAVRDDGTSGSY
                     VPEIYPAVAHHHVLRALEKAAAIVEHRCHMGIVLSTDSYYGARFNSETAKMIGLLKKA
                     NTLCVEMESSTLLTLGGVFGLRTGSILTIREEIGEDETLKAQAGEVFEDGLEKCIQIS
                     IKTLELIIEKDRLFKFD"
     gene            complement(3912..>4149)
                     /locus_tag="PLD15_09910"
     CDS             complement(3912..>4149)
                     /locus_tag="PLD15_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB64266.1"
                     /translation="KERLAILRALQNTSYTPIAMSKRLKIPSGELNNHIEKLKKARLI
                     ESFLDGEHLKYTANPQEIKKIVDQSLKRLLEEGD"
BASE COUNT         1023 a         1021 c         1045 g         1060 t
ORIGIN      
        1 ctgaagaatt aaaccgtatt aaaacccgta ttgaaaaggc aaaggagtat ctggctaagt
       61 catcttacag aggagagaaa gtcagacttc tgatttacac cagcaaggaa cagatggagc
      121 ttgccgagta cataaaactc ttcctctcta agattggaat aaacgttgta gtcgatcctc
      181 agggccatga agcggtgacg gcggcgctca agaaagctga cttcgacatg tcgctcacca
      241 catacagtct ggggttccac ccggaaatgg ccttctatca tctccactct tcgagaggga
      301 cgatgcaaaa cggccaggtt tcaggtttca actacggagg cgtgaatata ccggagctgg
      361 atgagactct taacattatc tggactgctt tcgacgagaa ggtacagagg gactctttcc
      421 atattctcca ggagcaggtc agggagttcg ttccggtagt tcccatctgc atacccaaca
      481 ggctggaagc cttctcgcgc aagaaccttg acggctggag tatatcagaa accgagggtg
      541 tattgtcgac ggagacgttg aagaatctga aatcaaaata gggagtttgc catgactctc
      601 tcctatctgg tcaaaagaat cctcataatg atattgacgg tcttcctggc ggtgacgctg
      661 agtttcatcc tcatcaggtc cctgccggga gaccctgcgg agagatatta cggcgatccg
      721 aggttgcccg aagaaataaa agaggaaatc aaggccgttt tcggactcga caagcccttg
      781 atcacacagt acttcatatt catagggaac gcgttcaggg gtgatctcgg tgtctcgtac
      841 acataccgca gaaacgtggt cggtgtaatt ctcgaaaggc tcccatggac gctgatgcta
      901 accgtggtgc cgaccgtctt gagtatgttc gtggcgctct ggataggaac attcatagct
      961 ttcaataggg gcaagaagtt cgatcgcttc atgaggttct tcgctttcgg attgaactcc
     1021 gttccaacgt tctggcttgc ccttctgctg gtcatgggaa cggggttcta tctaaacata
     1081 tttcctctgc agggtatgtt cgatccgctg gagagcggta tggcgagagt gataagcata
     1141 ctccaccacg ccgctcttcc ctggataact ctcttcatcg tagggatgcc gagttttgcg
     1201 atacaggtca ggaacatagc gctgaacatc ctgggggagg atttcataat aacggcgaag
     1261 gccaaaggtc tgagagagca agaaattcgg cgcaaacacg tgctcagaaa cgctctcgga
     1321 ccacttttct cgcttcttac cctgagaata gcggggcttg tgggaggggc agtactgata
     1381 gagactatct tttcctggaa gggaatggga ctgctggtgc tggaggcttc caaaaattct
     1441 gactacccgc ttcttcaagc caccgtcctg atgaccatcg tactggttat tctggccaat
     1501 tttctggcgg acgttctcca ggccgtcttt gatcccaggg tgatttacac atgaaaaaat
     1561 acagaaaggt tttgagggac atttggaaag ctccagaggg aaaggttagt cttttcatac
     1621 tggtcttatt cgtttttctc gccgttaccg ccgactggat ctcgccttac gatccttatg
     1681 acattactca gagaggcttt ccgctggaag cgccttcgaa ggagtatccg ctcgggaccg
     1741 atggtttcgg agttgatata ctcagtcaga taattcatgg cagtagggtc tcgctaacaa
     1801 tagggtttgt cacaggaatc ggcatagcct tcatgggcgg cttcatcgga gtgatggccg
     1861 gcacttacgg cggcctggcg gatctctttt ccatgagagc agtcgatttc ataatggtgc
     1921 tacccggact gcccataatg atactattga tgacaacttt gggaagcagt ttctgggtga
     1981 tggtgatgat cttcgttctc ttcggctggg cgtcggtcgc cagggttacg agggcgatag
     2041 ttctctcaga aagaaagcga ggctacgtcg aggcggcccg gtgcgccgga gctaaaaaaa
     2101 gccacattct cctcagacat ttgctcccgg caacctattc tatactcttc gtcaccgcag
     2161 ccttcgcggc gggtggttcg attctggccg aggccggtct tgcctttctg gggttcggag
     2221 atccccggct ggtttcgtgg ggaaagatgt tgaattacgc ccgcacttac aacgcgatgg
     2281 ttctcggcgc gtggtggtgg attatattcc ccggcatcgc tgtttttctt acgtcctttt
     2341 cgataatgat gctgggagtt gcactggaaa agacctttaa tccaagattg agagagaggg
     2401 acaggtaaat ggtagagtac attgaaaaca cggcaaagtt cgaattcagg ggaaagagct
     2461 acgagtacag acttcacgaa atgccagaag agttctttcg ctggcaggtc ggcaacagac
     2521 tcaaaggttt gaagatcttt ctgggggaag aaaagggtat gccgaacttc tctccccata
     2581 ccgtagttat gaacacgctc gaatacgaaa atgaattctg catcaactca tgcataaaag
     2641 gactcggact ggttcccaga tccgataaaa ttcaggaact gggagagagg gccatggaac
     2701 tcataagaaa atcttacgag atcggagcgg acaaaacctt tagagagagg gtgggaatgc
     2761 tggttgaact ctattccaat cccgaaacgt tcgataggag cgttctctca tcgatggaga
     2821 tgtacaaaaa gagaacctac aagaacgttt tagaagaccc aagggccaac ctgcttttct
     2881 acgataacca aaccggctac agcgtaatgt tcagcgtggt gtgcgaattc gtgcccagag
     2941 gaggcgctta ttacaattac gttagtgcca tacacgacct gtttcatctt cccaaagacc
     3001 catcgataaa gacagacagg tacgatttcg catacaggtt ccacctggta gaagccttcg
     3061 aaaagactcc cggccccagg gccagcacca gaatctattg atcaatcaaa aatcggatgg
     3121 gccgatcccc agcggtcggc ccctttcaat cgaatttgaa gagtctgtcc ttctctatta
     3181 tcagttcgag ggtcttgatc gaaatctgta tgcacttctc caatccatcc tcgaagacct
     3241 caccggcctg agcctttaga gtctcatcct caccgatttc ttcgcgtatc gtcaggatcg
     3301 agccagttct tagaccaaac acacctccta gagttaggag cgttgaactc tccatctcaa
     3361 cgcataaagt gttggctttc tttagaagtc cgatcatctt cgcggtttct gagttgaagc
     3421 gagcaccgta atacgaatcg gttgaaagca ctatgcccat gtgacatctg tgttcgacta
     3481 ttgccgccgc cttctctaga gccctcaaaa catggtgatg ggcgactgca ggatagatct
     3541 caggaacgta cgaacctgac gttccgtcgt ctctaacggc accagtaacg ataacccctt
     3601 ctcccatttt tagttttttc tggagacttc cggcactacc cacccttata agggtatgga
     3661 cacctagcgc ccggagctct tcaaccgcga tagccataca gggacctccc atcccggttg
     3721 acattaccga gactcgctct cccgagattt cgccggtgat tgtgaaatat tcgcgatttt
     3781 tcgccacatg gaccgggttt tcaaaatact tcgcgatcct ttccacacgg ccacgatcac
     3841 ccggaagcaa cacgtactta ccgatctttt cggcatcact gatgcgaatg tgaaaagtag
     3901 ggctttccaa ttcagtcgcc ttcctccagc agcctcttca gagactgatc aactatcttc
     3961 tttatctctt gcggattagc cgtgtacttc agatgttcac cgtccaagaa gctttctatc
     4021 agtctggcct tcttgagttt ctctatatgg ttgttgagtt ccccggaggg aattttgagc
     4081 ctcttggaca tggcaatcgg cgtgtaggac gtattctgca aagccctgag gattgctagc
     4141 ctctctttg
//
LOCUS       DAOOLY010000224         4123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_26074_length_4123_cov_4.327188, whole genome shotgun sequence.
ACCESSION   DAOOLY010000224 DAOOLY010000000
VERSION     DAOOLY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26074_length_4123_cov_4.327188"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="PLD15_09915"
     CDS             <1..1767
                     /locus_tag="PLD15_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64267.1"
                     /translation="LTLIPLFLFAQTEELKAKLAANPADIESLEALLKIYEENYDDNS
                     YINTVIEVARALTEIPEGMVAIFEKTIEKALNNYYSEQAGELSKIYFDNFKNRKSLLY
                     HLRSLNSSWYLSESDVRYAVTSIENEDHLELYKYLYEESKELYPTDLSLIVSRILVED
                     FGESEFLIPYIENLFYEGDFSTAEELLMEYSQNLTEEPMNYFLKGLLALNYGNYDEAV
                     ELFLQGKSFNDEAALGKAIEILDYIYIPDYVSELFRAIVLSSDEASLQNNIQMYMIGH
                     EELDWIYSWLSRVPAPKKINELLEGEEVVISYNTLNMDYESGNVGFFDIEGNLFGSLT
                     DAIYGQFLDSRTFVYISLQNERVVVDGERKARFNGYTVYSLSPDRSKFLLLDNYGEKI
                     VMIGPELTQIWSADGDFGMVPVSWSPDGYRVAVSTDYDVFSIFNADTGEVLETVEDWY
                     DLVFLSNDNAYAIDYDGNLVDLATDETLKTAKTAVWAEFGSENKIIYFVPPEEYGDFS
                     YIALRVYDLETGTDRVLSNRVLWMDAPVPSIKIEGPYIYFTEKDANGMHRVLVMDYTT
                     NELLFESMPSYDVLIFPDVRPE"
     gene            complement(1876..3408)
                     /locus_tag="PLD15_09920"
     CDS             complement(1876..3408)
                     /locus_tag="PLD15_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPB64268.1"
                     /translation="MKVLMVYPEYPETFWSFKHALKFVSKRAAYPPLGLMTVSAMLPE
                     EWEKKLIDMNTDTLSDEDILNSNYVMISSMDIQFDSAKKVLQRCKDLGVKTIAGGPLF
                     TTRPEEFSEVDHLVLGEAEVTLAPFLHDLEKGRAKHIYRSDGFPDISNSPIPDWKLLD
                     MRKYSSMNIQYSRGCPYNCEFCDIVLLNGRIPRTKRAEKLIGEMEALYIAGWRGGVFI
                     VDDNFIGNKTKLKREILPAIAEWMKNRNYPFLLNTEASIDLSDDDELMKLMVDANFGT
                     VFVGIETTEEESLVECGKYQNRNRDLLSSVRKIQEFGLQVQGGFIVGFDHDRPSVFRN
                     MINFIQKSGIVTAMVGVLTAPTGTRLFLRLKDENRIASEFSGNNTSILTNIVPKMGLN
                     NLVDGYYKILRSIYAPKPYRQRVITFLKNYKPNSLRHVRPLSDNLKAFVKSLWVLGIR
                     EKGRINYWLLLLWTAFFKPSLFPLSVEFAIYGYHFRKSFSFGLYRENKTQPPSYAERD
                     SK"
     gene            complement(3876..>4123)
                     /locus_tag="PLD15_09925"
     CDS             complement(3876..>4123)
                     /locus_tag="PLD15_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64269.1"
                     /translation="LGTIFTIFSLSRAIGKKGKTTPAEDAARVVVGMLAVLVYVLLRG
                     IPYLLPVWIMNMAILIYRHLADMKALLVNHASKRNGR"
BASE COUNT         1069 a          973 c          987 g         1094 t
ORIGIN      
        1 ctgactttga taccgctctt cttattcgcc cagaccgaag aattgaaagc gaaactggct
       61 gccaaccccg cggatataga aagtctcgaa gcgcttctca agatatacga agaaaactac
      121 gatgacaaca gctatatcaa caccgtgatc gaagttgccc gggccctcac ggagataccc
      181 gaaggcatgg tggcgatctt cgagaaaacg atcgaaaagg ctctgaacaa ctattactcg
      241 gagcaggcgg gcgaattgtc gaagatatac ttcgataact tcaagaacag gaaatcgctc
      301 ctctaccatt tgcgcagtct caacagctcc tggtatctca gcgaatccga cgttcgctat
      361 gccgtgacat cgatcgagaa cgaggatcac ctggagctct acaagtatct atacgaagag
      421 tccaaagaac tgtacccgac cgatctctcg ctgatcgtgt cgaggatact ggtcgaagat
      481 ttcggagaga gcgagttcct gataccctat atcgagaatt tattctacga gggagacttc
      541 tcgaccgccg aggaactgct catggaatac tcgcagaacc tgactgagga accgatgaat
      601 tacttcttga aaggcctgct ggctttgaac tacggtaact acgacgaagc tgtggagttg
      661 ttcctccagg gaaagagttt caacgacgaa gccgcgctgg gaaaggctat agagatactg
      721 gattatatat atattcccga ttatgtttcc gagctgttca gggcgatcgt gctctccagc
      781 gacgaggcat cgctgcagaa taatattcag atgtatatga tcggccacga agagctagac
      841 tggatatact cctggctgtc gagagttccg gcgcccaaga agataaacga actgctggaa
      901 ggagaagagg tagttatctc ctacaacacg ctgaatatgg attatgagag cgggaacgtc
      961 ggtttcttcg atatcgaagg taatctcttc gggagtctga cagatgcgat atatggccag
     1021 ttcctcgatt caaggacatt cgtctatata tcgctgcaga acgagagggt cgtggttgac
     1081 ggagagagaa aggccagatt caacggctac accgtctatt cgctttcccc ggatcgctcg
     1141 aaattcctgc ttctggacaa ttacggcgag aagatagtaa tgattggacc ggaattgacg
     1201 cagatctggt cggccgacgg agatttcgga atggtaccgg tctcctggtc gcccgatggt
     1261 tacagggtgg ctgtctccac cgattacgat gtcttctcca tattcaacgc agatacgggc
     1321 gaggtgctcg agaccgtgga agactggtac gatctcgttt tcctctccaa cgacaacgct
     1381 tacgcgatag actacgacgg caacctcgtc gacctggcga cggacgagac gctaaaaacg
     1441 gcaaaaacgg ctgtctgggc ggagtttgga agcgagaaca agatcatcta ttttgttccg
     1501 cctgaggagt atggcgattt ctcttacatc gccctgcggg tgtacgatct tgaaaccggc
     1561 accgaccggg tactcagtaa cagggtactc tggatggacg cgccggtccc ttcgatcaag
     1621 atagaagggc cttacatcta cttcacggaa aaggatgcca acggtatgca cagggttctg
     1681 gtcatggatt acaccaccaa cgaacttctc tttgaaagca tgccgagcta cgatgttctg
     1741 atcttccccg acgtcaggcc ggaataaaaa agccgttgcg aaccatcaaa acaaggccgg
     1801 agggtaaatg ccctccggtt ttttctggta gagctttaga aggagccatc gagaatcgct
     1861 gacatcagtc tgcggtcact ttgaatctct ttctgcgtaa ctcggtggtt gtgttttatt
     1921 ctctctgtat aacccaaaag aaaaggattt tcggaagtga tagccgtata tcgcaaattc
     1981 aactgacaat ggaaaaagcg atggcttaaa gaaggccgtc cagaggagca acaaccaata
     2041 gttaattcga cctttttccc ttattccaag tacccacagc gacttaacga aagccttcag
     2101 attgtcggaa aggggcctga catgtctaag tgaattcggt ttgtagttct tgagaaaggt
     2161 tattactctc tggcgatagg gtttcggcgc gtagatgctt ctgagtattt tatagtaacc
     2221 gtctacaagg ttattgagac ccatcttggg aactatgtta gtcagaatgc tggtgttgtt
     2281 gccggaaaac tccgacgcga tcctgttctc atctttcaac ctcaggaaga gtcttgtgcc
     2341 cgtaggcgct gtgaggacac caaccattgc cgtcactata ccgctcttct ggatgaaatt
     2401 gatcatgttc cgaaataccg aaggcctgtc gtgatcaaag cctacaataa atccgccctg
     2461 aacctgcaga ccgaactcct gtatcttcct tacagaggat agcaagtctc tgtttcggtt
     2521 ctggtacttg ccgcattcaa cgagactttc ctcctcggta gtctctatgc cgacgaagac
     2581 ggttccaaaa ttcgcgtcga ccatgagttt catcagttca tcgtcatctg aaagatcaat
     2641 tgaggcttcc gtattcaaca aaaacggata attcctgttt ttcatccatt cggcaatagc
     2701 cggaagaatt tctctcttca gtttagtttt atttccgatg aagttatcgt caactatgaa
     2761 tactcctcca cgccagccgg ctatatacaa cgcttccatt tcgccaatga gtttttccgc
     2821 tctcttcgtc ctgggaatac gtccgtttag cagaacaatg tcgcagaact cgcaattata
     2881 aggacaacct ctagaatact ggatgttcat tgaagagtac tttctcatgt caagaagctt
     2941 ccagtctgga atgggactgt tagagatatc tggaaagccg tcggatctgt aaatgtgctt
     3001 agcccggcct ttctctaaat catgaaggaa cggcgccaga gtcacttctg cttcaccaag
     3061 aactagatga tcgacttcac tgaactcctc tggtctagtt gtgaaaagcg gcccgcccgc
     3121 tatagttttg acacctaaat ctttgcacct ctgaagaacc ttctttgctg aatcgaactg
     3181 aatatccatt gaactgatca ttacgtaatt ggagtttaag atatcttcgt cacttagagt
     3241 gtccgtgttc atatcgataa gtttcttctc ccactcttca ggcaacatag ccgaaacggt
     3301 cattagtccg agtggtgggt acgctgccct ttttgagacg aattttaatg cgtgcttgaa
     3361 actccaaaat gtttctggat attctggata gaccatcaaa accttcatgt ctccacttcc
     3421 ctttttgtga cggcactcaa acctttgcta aaaactactt gctctacaag ttgatgcatt
     3481 gtgcgggatc aataattgct gaggtttgta actgttggaa agggtaatca tcggttgaag
     3541 gtcgaaatat acaattcatc ccgcgttgcc atctaatcaa tattatcaca acggaccaat
     3601 caaaagagct aatcactcaa cctatgcgtt tcgaggtgca taagttgcga ttatggtttc
     3661 tttggcctac cgacagacga taccatcctc gttatgagtt ggcgggaacc gaggttaaaa
     3721 aacggtttgt ttacgataaa atattcaggg aggtgatttt atgcttactt tgatatcgct
     3781 ggccgtcggt gttgtcctgg tgacggttct cggtgttgtt ttttatttcg taatcgccag
     3841 atgattccgg tccgttttgg tatttttcat ttgcttcacc ggccgtttct ttttgaggcg
     3901 tggttgacga gaagcgcttt catatccgcc aggtgcctgt agataagtat cgccatgttc
     3961 atgatccata cgggcagcag atagggaatg ccacgcagaa gaacgtaaac cagaacggcg
     4021 agcatgccaa ccacgaccct cgcggcgtcc tcggcggggg ttgttttacc tttcttgccg
     4081 attgccctcg agagagaaaa gatggtgaag atcgttccga gca
//
LOCUS       DAOOLY010000225         4024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_26957_length_4024_cov_4.194759, whole genome shotgun sequence.
ACCESSION   DAOOLY010000225 DAOOLY010000000
VERSION     DAOOLY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26957_length_4024_cov_4.194759"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLD15_09930"
     CDS             <1..682
                     /locus_tag="PLD15_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB64270.1"
                     /translation="SGSFKAAQSVEKVNLKSHIEERFEVPTFVSNDVTLNALGEMWAG
                     AAKGCRNVFLTALGTGTGAGLIINNELYEGTSGMAGEIGYMITDWSREKNLSFVFGSL
                     ESWFSGYAFEKLLSEFENEPTVAQMFDYSDINPRFKEIVTVACEHLSVVVANVITLLD
                     PDVVVITGGIGYNQYDRILSLIMPVLKRTVPGEILERVTFKRGELGELGVSLGAVCNV
                     QRKVFMEV"
     gene            711..1955
                     /locus_tag="PLD15_09935"
     CDS             711..1955
                     /locus_tag="PLD15_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64271.1"
                     /translation="MKKALLLLLVVSVIFAGFAGAVKITFWQYYYETKVKLVDQLIEE
                     FEKQNPGIEVEQVTFPYENFNEKVAVSVPAGVGPDVLTLFYGWIPLYVTSGYLVPLPA
                     AEFSAEYLSSEFFPFVSKGIEFDGAPYALPTAVRALALFWNKDLFVEAGLDPETPPTT
                     FAELVDYAKKLTKYDDKGNIIQEGLTMQPSGQGHQWIREVLVRQVGGTPYTADNRTVL
                     YHLTAPEALKVYTDLITKDKVGYPGFLNDDVTAFRSLKAAMTIDGSFRLGTFRPLTNL
                     KWGVTELPSMNGVKSNFASFWAHAITTNATGEKLDASIKFLKFITSEYAQKLWLEQVG
                     ELPANPNVAAAYYDHPEYGPFLKGLEYAHATFFVDETGQRQVMMDAVDKVWIQGMDPV
                     KAFKEAAEEDQAIIDEFWSRVE"
     gene            2012..2899
                     /locus_tag="PLD15_09940"
     CDS             2012..2899
                     /locus_tag="PLD15_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64272.1"
                     /translation="MKISQKRIVTAYVFLAVPLVFYIVVRFYPMIYAFWLSFTNWKLV
                     SPKQDFVGFSNFVKLFKDKVFMTSLWNTIKYVLFGVPLVIVISLFLAATLNRVKHLQW
                     FYRLLYVMPYITPLVAVSWVWRWIYQRPPAGILNNLLMAFGLDAQPFLTSMNQSLPAI
                     VVTTVWVNLGYCIIIFLAGLQTIPQEYIEAAKIDGATSRQSFFKITIPLLSPITVFLV
                     VTQSITFLRIFTQVYNMTDQGSGGPLNATKPLVLYIYQKAFRSFDMGLASSATVILFL
                     IIMGITLVQLLVLNKRVQY"
     gene            2915..3736
                     /locus_tag="PLD15_09945"
     CDS             2915..3736
                     /locus_tag="PLD15_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64273.1"
                     /translation="MTRTDRKTSFFAYIFLTIFAIVMVFPFVWMFSSSFKTSEEIYEM
                     TVIPENPSLANYEFIFNYSMFPRWFLNSMIVAVVTTISVLFFDSLVGYTLAKYNFPGK
                     NIIFILILSTLMIPTEMLVIPWYIMVRQLKWMETYWSIMFPGMISAFGVFLMKQFMST
                     IPDDLLDAARIDGVSEFGIFMRIVMPLVKPALATLAIFNFIGNWNAFLWPLIVAQTPK
                     MYTLPVGVSYFSSENLTRWELIMTGAAVSTIPLIIFFIIFQKQIVKGIAMSGLKG"
     gene            3742..>4024
                     /locus_tag="PLD15_09950"
     CDS             3742..>4024
                     /locus_tag="PLD15_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64274.1"
                     /translation="MRIIDSHIHFPTPDFFGKSEGSVHPWLAQERARWRKAWQFDGAE
                     ALRSQDELVERWYREAIEHDISVVFVTSGGNDAMSEIVKAHPDRFYGYAH"
BASE COUNT         1012 a          975 c         1004 g         1033 t
ORIGIN      
        1 ttcaggtagc tttaaagctg ctcaaagtgt cgaaaaagtc aatttgaaga gtcacatcga
       61 agagaggttc gaagtcccta ccttcgtttc gaacgacgtt acactcaatg ccctgggaga
      121 aatgtgggcc ggagccgcga agggctgcag aaacgtcttt ttgactgccc tcggtacggg
      181 gacgggcgcc ggattgataa taaacaacga actttacgaa ggcaccagcg gcatggccgg
      241 tgaaataggc tacatgataa ccgattggag cagggaaaag aacttaagtt tcgtctttgg
      301 aagcctggaa agctggtttt ccggatacgc ctttgaaaaa ctcctttccg agttcgaaaa
      361 cgaaccgacg gttgcacaga tgttcgacta ttccgatata aatccccggt tcaaagaaat
      421 agtcacggtc gcctgtgaac accttagtgt ggtggttgcg aacgtcatca cactgctgga
      481 tccggatgtt gtcgtcataa ctggtggaat cgggtacaac caatacgata ggatactcag
      541 tctaataatg ccggtactaa agaggaccgt tcccggtgaa atactcgaac gcgtgacatt
      601 caagcgcgga gaactcggtg agctgggcgt ttcgcttgga gctgtctgta atgttcaaag
      661 aaaagtcttt atggaagtat gagctacatc actattttgg aggtgggtaa atgaaaaaag
      721 cactattgtt gttgctggtt gtttccgtta tcttcgccgg tttcgccggt gcagtgaaga
      781 taactttctg gcagtactac tacgaaacga aagtgaaact ggttgatcag ttgatcgagg
      841 agtttgaaaa gcagaatccg gggatcgaag tagaacaggt tactttcccc tacgagaact
      901 tcaacgaaaa agtcgctgtg tcggttcctg ccggagtcgg accggatgtt ctcacccttt
      961 tctacggatg gattccactt tacgttactt ctggatacct ggttccgctt cctgcggctg
     1021 aattctctgc tgaatacctg agcagtgaat tcttcccgtt cgtgtcaaaa ggaatagagt
     1081 ttgatggtgc accgtacgct ctcccgactg cggtaagggc tctcgcgctt ttctggaaca
     1141 aggatctctt cgtcgaagcc ggtctcgatc cagaaacccc gccgacgact ttcgccgagc
     1201 tggtcgatta cgcgaaaaag cttaccaagt acgacgacaa gggtaacata atccaggaag
     1261 gtctgactat gcagccttcc ggccagggtc accagtggat aagagaggtt ctcgtcagac
     1321 aggtaggtgg aacgccttac accgctgaca acagaaccgt tctttaccat ctgacagctc
     1381 cggaggccct gaaggtatac accgacctga tcaccaagga caaggttggt tacccaggct
     1441 tcctgaacga tgacgttacg gccttcaggt ctctgaaagc cgcgatgact atcgacggtt
     1501 ctttcagact cggaaccttc agacctctca ccaatctcaa gtggggagta acggagcttc
     1561 catcgatgaa cggcgtgaag tccaacttcg cctctttctg ggcacacgcg ataacgacaa
     1621 atgcgaccgg cgaaaagctg gacgcttcga tcaagttcct caagttcata acttccgaat
     1681 acgctcagaa actgtggctc gagcaggtgg gcgaacttcc ggccaacccg aatgttgccg
     1741 ccgcctacta cgatcatcct gaatacggac cgttcctcaa gggtctcgag tacgcccacg
     1801 caactttctt cgttgacgag accggacagc gccaggtaat gatggatgcc gtcgataagg
     1861 tctggataca gggaatggac cccgttaagg ctttcaagga agcggccgaa gaagatcagg
     1921 caataatcga cgaattctgg tcgagagtag agtaatttga agagcgggtg gccgtggcgt
     1981 cgccacggcc attctttttg ggggtaaaat aatgaagata agtcagaaga ggatcgtaac
     2041 cgcatatgtt ttcctggctg tcccgctggt tttctatata gttgtccgtt tctatccgat
     2101 gatatatgct ttctggttga gtttcaccaa ctggaagctg gtttcaccca aacaagattt
     2161 cgtcggtttc agtaatttcg ttaaactctt caaagacaag gtgttcatga cctcgctatg
     2221 gaacactata aagtacgttc tgttcggtgt tccactcgtg atagtcattt cgctctttct
     2281 ggccgcgaca ctcaacaggg tgaaacatct acagtggttt tatcgcctgc tttacgttat
     2341 gccatacatc acaccactgg tggcggtgag ctgggtctgg agatggatct accagagacc
     2401 gcccgcggga atacttaaca accttttgat ggccttcggt ctcgacgcgc agccttttct
     2461 gaccagcatg aaccagtccc tgccggccat agtggtaaca acggtctggg taaatttggg
     2521 atactgtata atcatatttc ttgccggatt gcagaccata ccccaggagt acatagaggc
     2581 agccaaaata gacggggcga catcaagaca gtctttcttc aaaatcacca tacctctcct
     2641 tagtccgatc accgtatttc tcgtggttac acagagtatt acgttcctga ggatcttcac
     2701 gcaggtttac aacatgaccg atcagggatc gggaggtcct ctcaatgcga ccaaacccct
     2761 ggtgctttat atatatcaaa aggccttcag atcttttgac atgggtctcg cttctagcgc
     2821 aaccgtcata cttttcctga taataatggg tatcacgctg gtgcaactgc tggtattgaa
     2881 caagcgggtc caatactgat tggtgggtgt atatatgact aggacggaca ggaaaacaag
     2941 tttttttgcc tatatatttt taacgatctt tgcaattgtg atggtcttcc ccttcgtctg
     3001 gatgttttct tcgtctttca aaacatcaga ggagatctac gagatgaccg taatacctga
     3061 gaacccatcg ctggccaact acgagttcat tttcaactac tccatgtttc ccagatggtt
     3121 cctgaacagc atgatagtgg ccgtggtgac gactatctcc gttctctttt tcgactcgct
     3181 ggtgggctac acactggcaa aatacaattt cccgggcaag aacatcattt tcatactcat
     3241 actcagcaca ctcatgatcc cgaccgagat gctggtcatt ccgtggtaca tcatggtcag
     3301 gcagttgaaa tggatggaga cctactggag cataatgttc ccgggtatga taagcgcctt
     3361 cggtgtgttc ctcatgaaac agttcatgtc gaccataccg gacgacctgc ttgacgcggc
     3421 gagaatcgac ggagtttcgg aattcggcat attcatgaga atagtgatgc ctctcgtcaa
     3481 gccagcgctt gcaacgcttg cgatcttcaa cttcatcggt aactggaacg cttttctgtg
     3541 gcccctcatt gttgcacaga caccgaagat gtacactctt ccggtcggtg tgtcttactt
     3601 ctccagcgaa aacctcacca gatgggagct cataatgacc ggtgccgccg tttcgactat
     3661 accgctgatc attttcttca tcatcttcca gaagcagatc gtgaagggga tcgccatgtc
     3721 gggattgaag ggataaaagc catgcgaata attgattctc acatccattt tcccacaccg
     3781 gatttctttg gaaaatcaga aggcagcgtt catccatggt tggcccagga aagagcgcgc
     3841 tggagaaagg cctggcagtt cgacggagcc gaagccttgc gttctcaaga tgaacttgta
     3901 gagagatggt acagagaagc aatcgagcac gatatctcgg tagtctttgt gacttctggc
     3961 ggtaacgatg caatgtcgga gatagttaag gctcaccccg acagattcta cggttacgct
     4021 catc
//
LOCUS       DAOOLY010000226         4015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27041_length_4015_cov_4.423990, whole genome shotgun sequence.
ACCESSION   DAOOLY010000226 DAOOLY010000000
VERSION     DAOOLY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27041_length_4015_cov_4.423990"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLD15_09955"
     CDS             complement(<1..197)
                     /locus_tag="PLD15_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64275.1"
                     /translation="MKKLMKYLKPYWVFVVLAPALMMLEVAIDLFQPRLLEEIIDVGI
                     KQNNLDVVVNTGLKMLLITFIG"
     gene            455..1840
                     /locus_tag="PLD15_09960"
     CDS             455..1840
                     /locus_tag="PLD15_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HPB64276.1"
                     /translation="MAVFRIRGVVEGFYGKPWSMKDRLDIVQFMGNHGYNLYIYAPKD
                     DELQRFRWRESYDDDFVKGFSMVVERGRQYGVDVAMAVSPGLTVEYSSEVDLHTFIEK
                     LLSFTGMGIKNFALFYDDISFRLSSERDKERFNSLAEAQVFFANRVFEALKRELPAFD
                     FIICPTQYHGRAESEYIRELGEGLLPDIKIMWTGPQVCSQHIPESDAIKVSEAFKREI
                     LYWDNYPVNDGSMIPEMHIGPYVGRDPEIVNHSCGIVLNPMNQAHASMIVLGAAADFL
                     NDPHSYDPTISWVNAMRKLTPGLIDEMLLFGEYSLISPIHPDQSERPRKVIEEFRTLY
                     SQGRVEEAIVWLEKEARTILDSAKNLKDKLPSLFLEEMALWIYEFERWGEALSVTVEI
                     LKSRMKLYVEIPEKKDLQQVKKLIEKAEKRMMELTRARTLSGGCAYREFIMEILIRTK
                     GYFTLKERNWL"
     gene            complement(2054..2815)
                     /locus_tag="PLD15_09965"
     CDS             complement(2054..2815)
                     /locus_tag="PLD15_09965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB64277.1"
                     /translation="MIDLKKALITGASSGIGREFARELASNGYETILVARRYDRLKDV
                     SEELTAQFNSPSTPVQADLSQSVDLERISGYLSGVDLLVNNAGFGLIGSFSELESEKE
                     LEMIHLNVESLYRLSKGYVIARKGQGGGIINVASTAAYLPVPFMAVYAATKAFVLSFS
                     EALSEELKDSNFKVMVLSPGPTSTEFFEVASGKNRKMYGAMTPDKVVKKAMAAYEKSK
                     RSIVSGFSNIVVAGGSRIAPKGLLLKVAGRAFRDF"
     gene            2930..3493
                     /locus_tag="PLD15_09970"
     CDS             2930..3493
                     /locus_tag="PLD15_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64278.1"
                     /translation="MKKLALFLILLFVVTSFATWMPLEDYSGVKYVYYKINYTQYEEE
                     KEMIYGVEIGMDEEKYILNYSTTVFLPKDQPLGSDVLFEQELSMFMYTFLNPMFSFFY
                     EAIDLNEQMNTKIFGFGSIKYEGQVTVQSKNNEYTGTKVVLYNEDNELSMYWVLNQDI
                     PFPIITYTGDDYSDGSTIVQLWDYELR"
     gene            3573..>4015
                     /locus_tag="PLD15_09975"
     CDS             3573..>4015
                     /locus_tag="PLD15_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB64279.1"
                     /translation="MNLVEIRHKLHMMPEIAFREFKTQAYIKELLTDFAIEYEEVAGT
                     GILARWEGGEGPYILFRADMDALPMSEETGCEFSSRNEGFMHACGHDVHMSVLLGLVE
                     RVSIYRPPVNILFLFQPAEEAGGGAELCLKPLEKYPIEEVWALHVT"
BASE COUNT         1134 a          881 c          980 g         1020 t
ORIGIN      
        1 cctatgaacg ttatcaataa catcttcaat cccgtattga caactacgtc caggttgttc
       61 tgctttatgc cgacgtcgat tatctcttca agtaatctcg gttgaaaaag atctatggcg
      121 acttccagca tcatcaatgc cggtgccaga actacaaaaa cccagtaggg tttgagatac
      181 ttcataagtt ttttcatcaa catacctcaa tatacctcta acagcaaggc ctggaaaaag
      241 tgaatctcgt cgaacatgaa tttcaagaag tccgatttgc gatcaactaa agattataca
      301 ttatccagga agcttaatga tcgttgtggt tttcaccaga gctttgagaa aacctttctg
      361 aatttccaga acctttgaca cgagaaaatt gtggagcaag aatgtgctag actttatcaa
      421 atccagaaca aaatggcgaa aggtgtgatc gaaggtggca gtatttagaa taaggggagt
      481 ggtagagggc ttctacggga agccctggtc tatgaaggat cgactcgata tcgtccaatt
      541 catgggcaat cacgggtaca acctgtacat atatgctccg aaagacgacg aacttcaaag
      601 gtttcgctgg agggaaagct atgacgacga ttttgtgaag ggtttttcca tggttgtcga
      661 aaggggcaga caatacgggg tcgatgtggc gatggcggtc agccccggtt tgacggtcga
      721 gtattccagc gaggtcgacc tccatacatt catcgagaaa ttgctgagtt ttaccgggat
      781 gggaatcaag aacttcgccc tgttttacga cgatatatcc ttcaggttat ctagcgaaag
      841 ggataaagag aggttcaact cgctcgcgga ggctcaggtg ttttttgcca acagggtttt
      901 cgaagctttg aaaagagaac tgccagcttt cgattttata atctgcccca cccaatacca
      961 tggcagagcc gaatccgaat atatccggga gctgggcgag gggctcctgc cggatatcaa
     1021 gataatgtgg accggtccgc aggtctgttc gcagcacata cccgaaagcg acgccataaa
     1081 ggtcagcgaa gccttcaaga gagaaatact gtactgggac aactatcccg taaacgatgg
     1141 gagcatgata ccggaaatgc atatagggcc atacgtcggc agggatccag aaatcgttaa
     1201 tcactcttgt ggtatagtgt tgaacccgat gaaccaggcc cacgcttcca tgatagttct
     1261 cggtgcggcg gcagactttt taaacgaccc ccatagttac gatccaacga tctcctgggt
     1321 gaatgctatg agaaagctca ctcctggatt gatcgacgag atgctcctct tcggcgaata
     1381 cagcctaatc agtccgatac atcctgacca atcggaaaga ccaaggaaag tgatcgaaga
     1441 gttcagaacg ctttactccc agggaagagt tgaagaggcg attgtgtggc tggaaaaaga
     1501 ggcacgaacg atactcgatt ctgcaaaaaa cctgaaagac aaactcccat cgctgtttct
     1561 tgaggaaatg gccctctgga tatatgaatt cgagcgctgg ggcgaggcgc tctcggtgac
     1621 tgtggaaatt cttaaaagca gaatgaaact gtatgtcgaa atacccgaaa agaaagactt
     1681 gcagcaggtg aaaaaactca ttgagaaggc ggaaaaaaga atgatggaac tgaccagagc
     1741 cagaactctc tctggaggat gcgcataccg ggagttcatt atggaaatcc tcatacgcac
     1801 gaaaggttac tttaccctta aagagagaaa ctggctttga acactggatc tgcaatcgaa
     1861 ctcgatttgc tctgaagact aaactaaggt ggctacaggg aatgcataat tcactacctt
     1921 tattctttat taggaagcag gctactgaga ccacaatgtg atactcacgg gaaactcttc
     1981 ccgtattgat acgaagctta ctgggagagt tcgagatcac atttttatga atcggctgtc
     2041 tggtcgctat aagtcaaaag tcgcgaaatg cccttcctgc aacttttaga agcagtcctt
     2101 ttggagcgat tctcgatcct ccggcaacta ctatgttcga gaatccgctc actatggatc
     2161 tcttgctttt ttcataagcc gccatggctt ttttcaccac tttatccggg gtcatggcgc
     2221 catacatctt tctgtttttt ccggaagcga cttcgaagaa ttccgtgctg gtcggtccgg
     2281 gcgatagaac catgactttg aaattactgt ctttgagctc ttctgagaga gcttcgctga
     2341 aactcaacac gaaggccttg gttgccgcgt aaacggccat gaaaggaacg ggaagataag
     2401 cggcggttga ggctacgttg atgatgcctc caccctgtcc ttttctggct atgacataac
     2461 ccttcgacag ccggtaaagc gattcaacgt tgagatgtat catctcgagc tctttctcac
     2521 tttccagttc actgaaggaa ccgatcaatc cgaagccggc gttgttgacc agcagatcca
     2581 ctccggaaag gtagccggat attcgttcga gatcgacact ttgagagaga tctgcctgaa
     2641 ctggagtgga aggggagttg aattgcgcgg tcaactcctc cgaaacatct ttcaatctgt
     2701 cgtatcttct cgctacaagt attgtctcgt aaccattcga agccagttct cgggcgaact
     2761 ctcttccaat gcctgacgaa gctccggtta tcaatgcctt tttcaaatct atcacctcgg
     2821 gatgttagac ttgcactcca atgaacaagt ttgtgtcgat agcatggtat taatcgacaa
     2881 aagcgatata atatacagga tacatagaaa tctgaaaggg gtgaattttg tgaagaaact
     2941 ggcactattt ctaattttac tgttcgttgt cacgtctttt gcaacctgga tgcctctgga
     3001 agactacagt ggcgtcaaat acgtttatta caaaatcaac tacactcagt atgaagaaga
     3061 gaaggaaatg atctacggtg tagagatcgg aatggatgag gagaaataca tcctcaatta
     3121 cagtacaacg gtttttcttc caaaagatca gccgctcggc agcgatgtct tgtttgaaca
     3181 ggaactttcc atgttcatgt acacattttt aaacccgatg ttttcctttt tctatgaagc
     3241 catcgatctg aatgagcaga tgaacacaaa gattttcgga ttcggttcga taaagtacga
     3301 agggcaggta actgttcaga gcaagaataa tgaatacacc gggactaaag tcgtacttta
     3361 caacgaagac aacgagcttt ccatgtactg ggttctcaac caggatatcc ccttcccgat
     3421 aatcacttac acgggcgacg actattcaga tggttcgacc atagtgcagc tttgggatta
     3481 tgagttgaga tgaacatatg aaatgaaaag gcgccttaaa ggcgcctttt ttgtttataa
     3541 tctaacaaat aagggagatc gaggaggcag gaatgaatct ggtagaaatt agacacaaat
     3601 tgcacatgat gcctgagata gcttttaggg agttcaagac acaggcttac ataaaggaac
     3661 ttctgacgga ctttgcaatc gagtacgagg aagtcgcggg gactggcatc ctggccagat
     3721 gggagggcgg cgaaggacct tacattttgt tcagggccga catggacgcc ctgcccatga
     3781 gcgaagagac cggttgcgag ttttcctcga gaaacgaagg gttcatgcat gcctgtggtc
     3841 acgatgttca catgtccgtg ctgctgggat tggtggagcg agtttcaatt tacagaccgc
     3901 cagtaaacat actgtttctc tttcaaccgg ccgaagaggc cggcggaggc gccgagctct
     3961 gtctgaagcc gctggaaaaa tacccgatcg aagaggtgtg ggcgctccat gtaac
//
LOCUS       DAOOLY010000227         4013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27054_length_4013_cov_5.027539, whole genome shotgun sequence.
ACCESSION   DAOOLY010000227 DAOOLY010000000
VERSION     DAOOLY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27054_length_4013_cov_5.027539"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /gene="ftsH"
                     /locus_tag="PLD15_09980"
     CDS             complement(<1..1401)
                     /gene="ftsH"
                     /locus_tag="PLD15_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB64280.1"
                     /translation="MEQRPRLGMILFYVVLGIFLLVALRGLYPSETSVEVPYTKFLDD
                     FNNIIDIVIYDNGKVVYVTGDNPRRSLETYFPSQTLITQTFQDQIDQLAGRGVNITFE
                     RGGDSLFWVNLLGTIIPLAIIVFIWFFAMRSLSGRNSQAFTFTKSPAKKYTSSDKRVT
                     FKDVAGVDEAQQELADVVSFLKDPGIFTDTGARMPKGILLVGPPGTGKTLLARSVAGE
                     AGVPFFFISGSDFVELFVGVGAARVRDLFAQAKVSAPAIVFIDEIDAVGRHRGAGLGG
                     GHDEREQTLNQILVEMDGFDAKTGVIVIAATNRPDILDKALLRPGRFDKKISVDPPDL
                     NGRAAILKIHMRGKPIDPDVDVQLLARRTPGFVGADLENLINEAAILSARKKKKMIGM
                     FELEEAIDRVLAGPAKKSRIISDKEKKILAYHELGHAVVGLVLPNAFPVHKVTIIPRG
                     TSTLGFTESLPLEDRYL"
     gene            complement(1406..2785)
                     /gene="tilS"
                     /locus_tag="PLD15_09985"
     CDS             complement(1406..2785)
                     /gene="tilS"
                     /locus_tag="PLD15_09985"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPB64281.1"
                     /translation="MHVLEEETLGFIGRWNLLKPGQRVLVAVSGGKDSMALLNILWNL
                     SPLLSIELVAANLDHGLRERGPSEEAEMIKRICIEKGIAFFHERRDVKDYMSKVPGLS
                     VEEAARKVRYDFLREVKNAQLCDVIAVAHNRDDLTETMLLRMARGTGLKGVVGLRPSN
                     GDIVRPLLFCDMQRIMDYVTINMVTFMEDETNLDTSFDRNSVRLKIVPEFKRLNPSFN
                     EAMWRFFENLYDGYSIVERDVKEALSSFKAIDGILFVESVILKGVYRALLFETVREIV
                     SRMSSDGYPPSRERVEAFYDLLMSDRNGWTVEFREDLKSSRLGDYVFFYKKGLVFETL
                     EAVEVFSLPFEFSHGVWRLRIREGGKPFDRLDGRFLSVCGIGRMALPLKLRPVDKKDS
                     IVPFGMSRTRRVERIVAEKGMKGFWSKLMVLENAKGEILWVPGVVTSELCRADPSYTE
                     LAVFSLERR"
     gene            complement(2831..2906)
                     /locus_tag="PLD15_09990"
     tRNA            complement(2831..2906)
                     /locus_tag="PLD15_09990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2871..2873),aa:Thr,seq:cgt)
     gene            complement(2952..>4013)
                     /gene="rny"
                     /locus_tag="PLD15_09995"
     CDS             complement(2952..>4013)
                     /gene="rny"
                     /locus_tag="PLD15_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPB64282.1"
                     /translation="EHDLAQKLKQLKDQYDEEGSRYAKWVIVNSIQRYAADYTGDVTV
                     STVSLPSDEMKGRIIGREGRNIRAFEKLTGSDLVIDDTPEVVVVSCFNPLRRAIAKMT
                     LDKLVEDGRIHPARIEEMYEKSKKEIYNEIKEAGQEALMKVGIPSMHPELVKLLGRLK
                     FRTSYGQNVLQHSIEVAHLAALMASELGLNVDKVKRGAILHDIGKAVDHEIEGSHSII
                     GGEIAKRFGEKTQVVNMIQYHHGETEAMTPEAVLVAAADAVSASRPGARRESLDMYIK
                     RLESLENIATSFKHIEKAYAIQAGREIRVIVEPEKVDDILAEKLAMDIAKKIEEELEY
                     PGVIKVTVIRERRSVSYAS"
BASE COUNT          926 a         1106 c          878 g         1103 t
ORIGIN      
        1 caggtagcga tcctctagcg gaagactctc ggtgaagccc aatgtcgaag tgccgcgtgg
       61 tatgatcgtt actttgtgaa cggggaaggc gttcggcaaa acaaggccta ccaccgcgtg
      121 tcccagttca tggtaggcga ggattttctt ctccttatcg ctgattatcc tggatttttt
      181 cgccgggccg gccaggactc tgtcgatagc ctcttccagc tcgaacatgc cgatcatctt
      241 tttcttcttt ctggcagaga gaatggccgc ttcgtttatc aggttttcca gatcggcacc
      301 aacgaagccg ggggttcttc ttgccagaag ctgcacatct acatcggggt cgatcggttt
      361 accgcgcatg tgtatcttca gaatagccgc tctgccattg agatctggtg gatcgacgga
      421 gattttttta tcgaatcttc ccggtcttaa aagagctttg tcgaggatat ccggtctgtt
      481 cgtagcggcg atcactatta ctccggtctt ggcgtcgaag ccgtccattt ctaccagtat
      541 ctggttgaga gtctgttcac gttcatcgtg accgcctccc aggccggccc ccctgtgcct
      601 gcctacggca tcgatttcgt ctataaaaac gatggccgga gcagatacct ttgcctgcgc
      661 gaagagatcc ctgactctgg ctgcaccgac accgacgaaa agctctacga aatccgagcc
      721 gcttatgaag aagaaaggaa ccccggcttc tccggcgacc gatcttgcca gaagagtctt
      781 tcccgttccg ggaggtccga ccagaaggat tcctttgggc attctggcac ccgtgtccgt
      841 aaatataccg ggatctttca ggaagctaac cacatcggcc agttcctgct gagcttcgtc
      901 aacaccggca acatccttga aggtcaccct cttatcgctg ctagtgtact tcttggccgg
      961 gctctttgtg aaagtgaagg cctgagagtt tctcccagaa agcgacctca tggcgaagaa
     1021 ccatatgaag actattattg ccagagggat tatagttccc agaagattca cccagaagag
     1081 cgaatcgcct cccctttcga aagttatgtt cacacccctt ccggcgagct gatctatctg
     1141 atcctggaag gtttgagtga tcaatgtttg agaggggaag taggtttcga gagaacgtct
     1201 ggggttgtcc cctgtaacgt acacaacttt tccattgtca tagataacta tatctattat
     1261 gttattgaaa tcatccagaa acttggtata ggggacttca acgctggtct ctgacgggta
     1321 cagaccccga agagcaacaa gcaagaatat gcccaaaaca acataaaaaa ggatcatccc
     1381 gagcctgggc ctttgttcca ctgaattacc tcctttcaag gctaaaaact gctaattcgg
     1441 tatatgaagg gtctgctctg caaagttcac tggtaacaac accgggaacc cagagaatct
     1501 cgccttttgc attttccaaa accatcaatt tgctccaaaa acccttcata cctttctccg
     1561 cgactattcg ttcaactctc ctggtacggc tcatcccgaa aggaacgata gaatcttttt
     1621 tatcgaccgg ccttagtttc aatggaaggg ccattcttcc gataccgcac acgcttaaaa
     1681 accgcccatc cagcctgtca aaaggctttc ccccttctct gattctcaac ctccagactc
     1741 catgagagaa ttcaaagggc aaagaaaaca cctctaccgc ctcaagagtt tcaaaaacca
     1801 gacccttctt gtaaaagaac acgtaatctc ccagcctgga gcttttgagg tcttctctga
     1861 attcgacagt ccagccgttt ctatcgctca taagaagatc gtaaaaggcc tcgaccctct
     1921 ctctagaagg cggataaccg tctgagctca tcctcgagac gatctctctc acagtctcaa
     1981 agagcaatgc tctgtaaaca cctttcaaaa ttacactctc aacgaagagg attccgtcta
     2041 tcgctttgaa cgacgacagt gcctctttaa catctctttc gactatcgag tagccgtcgt
     2101 aaagattttc aaaaaaacgc cacatcgctt cgttgaaaga cgggttaagc ctcttgaact
     2161 ccggcactat cttgagacgt acagagtttc tgtcaaagct ggtatccaga tttgtctcgt
     2221 cttccataaa tgtaaccatg ttaattgtaa cataatccat aatacgttgc atatcacaga
     2281 acagtaatgg tcttactata tcaccattcg acggtcttag accaaccact cccttcaatc
     2341 ctgttccccg tgccatacga agcaacatgg tttcggtcag gtcgtcgcgg ttgtgagcca
     2401 cggctattac atcgcacaat tgtgcgtttt tgacttctct caggaaatcg tatcttactt
     2461 ttcttgcagc ttcttcgacc gatagaccgg ggactttcga catgtaatcc ttcacgtctc
     2521 ttctctcgtg aaaaaaagcg atcccctttt cgatacaaat tcgctttatc atctcggctt
     2581 cttccgacgg tcctctttcg cgaagaccgt gatccaggtt ggctgcgacc agttcgatcg
     2641 aaaggagagg actcaggttc cacagtatgt ttaaaagtgc cattgaatct ttcccgccgg
     2701 aaacagctac gagaactctc tgaccgggct ttaacaggtt ccatcgtccg ataaaaccaa
     2761 gtgtctcttc ttcaagcaca tgcatccttt acaccaccat ctataacaat gcgcccatta
     2821 agggcgcatc tggagccaac gaagggaatt gaacccccaa cctgctcatt acgaatgagc
     2881 cgctctgccg attgagctac gttggcacct ggaatcaatt ttatatcacc cgacagtcta
     2941 tgtcaacgag ttcacgatgc gtaggaaacg ctcctgcgtt ccctgataac agttaccttt
     3001 atgactcccg ggtattccag ttcttcctcg atctttttgg ctatgtccat cgcaagcttt
     3061 tcggccagta tgtcgtcgac cttctccggc tcgacgatga cccgtatctc cctgccagcc
     3121 tgtatggcgt aagccttttc gatatgtttg aagctcgtgg ctatattttc cagactttcc
     3181 agtctcttta tgtacatatc aagcgattct ctcctggcac cgggtctgga tgcggaaacg
     3241 gcatcagccg ccgccactag aacggcctcg ggtgtcatgg cttcggtctc accgtggtgg
     3301 tactgaatca tgttcaccac ctgggtcttc tctccgaatc tcttggctat ctctcctccg
     3361 atgatcgagt gcgatccttc aatttcatga tctacggcct ttcctatatc gtggagtatg
     3421 gctccgcgct tgaccttgtc aacgttgagc cccagctctg aagccatgag ggcggccaaa
     3481 tgtgcgacct ctatggagtg ttgcaggacg ttctgaccgt aactggttct gaacttcagc
     3541 cgtccgagaa gcttgaccag ttcgggatgc atggaaggta tcccgacctt cataagcgct
     3601 tcctggccgg cttcctttat ttcgttgtag atttccttct tcgatttttc gtacatctct
     3661 tcgatgcggg ccggatgaat tctgccgtct tcaaccagtt tatccagggt cattttcgct
     3721 atggccctcc ttaggggatt gaagcatgag acaacaacca cttcgggcgt gtcgtcgatc
     3781 accaggtccg atccggtgag tttctcgaag gcccttatgt ttcttccctc tctacctatt
     3841 atccttccct tcatctcgtc ggacggcagg ctcacagtgc ttacggtcac atcaccggtg
     3901 taatcggccg catacctctg tatagaatta acgatgaccc attttgcgta acgacttccc
     3961 tcttcgtcgt attggtcctt aagctgctta agcttctgtg ccagatcgtg ctc
//
LOCUS       DAOOLY010000228         3978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27384_length_3978_cov_3.512108, whole genome shotgun sequence.
ACCESSION   DAOOLY010000228 DAOOLY010000000
VERSION     DAOOLY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27384_length_3978_cov_3.512108"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLD15_10000"
     CDS             complement(<1..270)
                     /locus_tag="PLD15_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HPB64283.1"
                     /translation="MTSLATHSISVYSNPQSLEKPLGDFCFKGYRRGMKLIFISNRIT
                     RSDFKKALSLYGLDLEEMCRSGQLIVSDKEESYLKGGLFKPENMLR"
     gene            complement(427..2793)
                     /locus_tag="PLD15_10005"
     CDS             complement(427..2793)
                     /locus_tag="PLD15_10005"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB64284.1"
                     /translation="MGTSGEHRKTKGLGDEMIALKELTDSIGDVFYIIDMKAHLIYVN
                     GNTEDMYGYSVGEMLALSFQEIDMSTVERFQRNCENATIGKSYSIETMHRRKDGTLFP
                     VRLNLTLIEKGSERLLVGLASDLSTRKNGEAEAAKISRTLPEFEDDFEPPELEDLIDF
                     QSIHSLMDDFYKLTKITISIIDNHGKSLVATGGRSICSILDCVDLQTPRNCMIDYLKM
                     AENVEAGAFRLYKCANNMWQMVTPIFAGDIRLGSIYLGQFFMEGEEIDYEFYRRQAKE
                     RGFDEEKCLEAIEGLPRLKVETVDTITSFYSKFANIVAVRSLSSMELTRTTAQRDRLN
                     KTVIDNEKRYALFVEHTDDLVLMINSDGILTYVSPAGERLFGYAMENLIGHLYGEFIH
                     PDDLKIVMDALGRSFNEGPHHTTSTLEFRIRHADGSWEWLSASGYVTKDCEDCPSYFI
                     GIARDITGRKNTEEALKRSEEEKSLILNATKETIVYYDRDLRINWINRAMAKRLGMEV
                     GDLIGKKCFTIWKAGEKCNDCILERVIKSREQKKMELTNEMGEFWLVRAYPVMGTDGE
                     VSGVVEVSEDITERKRASKKLEAAFDALVRTASDIVSARDPYTADHQRNVSKLAVAMG
                     REMGLNDEICESIRVAGLLHDIGKITVPTELLTRLGKLNEIEFDLIKEHSRSGYEILK
                     DVDLPWPVAEIVLQHHERLDGSGYPDGLKGEQIRLESKIIAVADVVETITSHRPYRSA
                     LGVDAALNEIINNSGKLYDPEIVKACLALFKRGYTFEFELGLPAVRER"
     gene            complement(3035..3880)
                     /locus_tag="PLD15_10010"
     CDS             complement(3035..3880)
                     /locus_tag="PLD15_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPB64285.1"
                     /translation="MKRNPALRISLLLIAFLLTSVWLFATEPEIGEMVLVERGTFIMG
                     DASENAEHGERPAHVVTLTYDFFIGKYEVTFDQYDVFCDATGRSRSGDRDWGRGLMPA
                     INVSWWDAIAYCNWLSENEELPLAYDINGNFLDTDGNITFDPSSVAGYRLPTEAEWEF
                     AARGGNESHNYARAGGNNVENVAWYKLNASSKTHEVGGKAPNELGLYDMSGNVWEWVS
                     DRYGLYGEAPLTNPFNGTEGTLRVNRGGSWIDCQMGVRIICRGSSLPTYANVNVGFRI
                     ARTER"
BASE COUNT          926 a         1042 c          836 g         1174 t
ORIGIN      
        1 tctcaacata ttctcgggtt tgaatagacc acctttcaga tagctttcct ctttgtccga
       61 cacgatcaac tgtcccgatc gacacatctc ttctagatcg agtccgtaca gcgaaagggc
      121 tttcttgaag tcgctcctgg tgattctgtt ggagatgaaa ataagcttca taccccttct
      181 gtagcctttg aaacaaaaat cgccgagagg cttctccaga ctttgtggat tgctgtacac
      241 agatatacta tgagtggcca acgatgtcat agcaatccgc ccctattcgg ttctctcgta
      301 tcaacacctc cgagatttaa acatgacaac ataccgaaat tataacactc agttattcca
      361 accagttact tttttttcac tcgaaaagta ttcaatttta gcactgtggt cggccgtaaa
      421 tgagatttat ctctctctaa cagccgggag ccccagttcg aattcgaagg tgtaacctct
      481 cttgaaaagg gccaggcaag cctttactat ctccggatcg tacaatttcc ccgaattatt
      541 gattatctca ttcaacgctg catctacgcc cagcgcgctg cgataaggtc tgtgagaggt
      601 tatcgtttct acgacatcgg caaccgctat tatcttcgat tcgagtctta tctgttcacc
      661 cttcaaacca tcggggtagc cggagccgtc gaggcgttcg tggtgttgaa ggactatctc
      721 ggcaaccggc cagggcaggt caacgtcttt aagaatctcg tatccactcc gtgaatgttc
      781 ttttataagg tcgaattcta tttcgttaag ttttcccagt ctggtcaaaa gctccgttgg
      841 aacggttatt ttgcctatat cgtgaagcaa gccggctacc cttatggatt cgcagatctc
      901 gtcattcagt cccatttccc tacccatcgc caccgcgagt ttgcttacgt ttctctgatg
      961 gtcggctgtg tagggatctc ttgcgctcac tatatcagac gcggtgcgaa ccaatgcgtc
     1021 gaaggctgcc tccagctttt tggaagcccg ctttctttcg gtgatgtcct cgctgacttc
     1081 cacaacgccc gatacctctc cgtccgtccc cataactgga taggcccgca caagccagaa
     1141 ctcgcccatt tcgttggtga gttccatttt tttctgctca cggcttttga ttaccctttc
     1201 cagtatgcag tcgttgcact tctcaccggc tttccagatc gtgaagcact tttttcctat
     1261 caggtcgccg acctccatgc ccaaacgctt cgccatagct ctgttgatcc agtttattct
     1321 gagatctctg tcgtaataaa ctatagtttc tttggttgcg ttgagtatca aggatttctc
     1381 ttcctcggac cgtttcaatg cctcttcggt gttttttctt ccggttatgt ccctggcgat
     1441 gcctatgaaa taactggggc agtcttcaca gtctttggtt acatagccgc ttgccgaaag
     1501 ccactcccag ctcccgtccg catggcgaat cctgaattca agtgtggatg tggtatggtg
     1561 ggggccctcg ttgaatgatc tccccagagc atccatgaca atttttaggt catcgggatg
     1621 aatgaactcc ccgtacaaat gacctataag attctccatc gcgtaaccga aaagcctttc
     1681 accggccggc gatacataag tcagtattcc gtcggagttg atcataagga ccagatcgtc
     1741 ggtatgctct acaaagagtg catatctctt ttcattatcg attacagtct tgttgagcct
     1801 gtctctctgt gcggttgtac gtgtgagttc catgctagag agacttcgaa cagccactat
     1861 atttgcaaac ttcgaataaa agcttgttat ggtatctacc gtttcaactt tcaatcttgg
     1921 caggccttcg attgcctcca aacacttctc ttcatcgaag ccacgctctt tggcctgacg
     1981 acggtaaaat tcgtagtcta tctcttcgcc ctccatgaag aactgaccga gatagatgct
     2041 tccaagacgt atgtcgcctg caaagatggg cgttaccatc tgccacatat tgttcgcgca
     2101 tttgtacaac ctgaaggcac cggcttcaac gttctcggcc atctttagat agtcgatcat
     2161 gcagttgcgg ggtgtttgga gatccacgca gtccaaaatg gagcagatac tgcgtccgcc
     2221 ggtggcaacc aggctttttc catggttgtc tattatcgag atagtaattt tcgtgagctt
     2281 gtagaaatca tccatcaacg aatgtataga ctggaagtct atcaggtctt ccaattcggg
     2341 cggttcgaaa tcatcttcaa attctggaag agttctcgat atctttgcag cttccgcttc
     2401 gccattcttt ctggtggaga gatcgctcgc caggcctacc aggagccttt cgcttccctt
     2461 ttcaatgagt gttaaattaa gccgtaccgg gaagagcgta ccatctttcc tgcggtgcat
     2521 cgtctcgata gaataggact ttccgattgt agcgttttca cagtttctct ggaatctctc
     2581 aacggtgctc atatcaattt cctgaaaaga tagggccagc atctcgccaa ccgaatagcc
     2641 gtacatatct tcggtattgc cattcacgta aatcagatgt gccttcatat cgattatgta
     2701 aaaaacatcg ccaatggaat ccgtcaattc cttaagggct atcatctcat ctcccagacc
     2761 tttagttttg cggtgttcgc ctgaggttcc catagctgta attttggttc gatgtcttcc
     2821 gccactttcg taaaggacaa taaacctgta ttgaacacct caattcaaga accactgcac
     2881 ttatatctta tcaactaaaa cggataaaac aataacggtt taataagttc gtatatgctt
     2941 catttattta taatattatc tcatccaaga agacgacaag aaatataaga ggcgactgct
     3001 tcgttttctc tcatatggta attgaaaatg gcttttatcg ctccgttctg gctattctaa
     3061 atccaacgtt gacgttggcg tatgttggca ggctgcttcc acggcagatt atccgaacac
     3121 ccatctggca atcgatccag ctacccccgc ggttgactct taaggtacct tccgttccgt
     3181 tgaaggggtt tgtcagcggt gcctcgccgt acagtccgta ccggtcgctc acccattccc
     3241 agacgtttcc gctcatatcg taaaggccga gttcgttcgg cgcttttccc ccgacttcat
     3301 gggtcttgct cgaagcgttc aatttgtacc aagctacgtt ttcaacgttg ttgccacctg
     3361 ctctggcgta gttgtgactc tcgtttcccc ctcgcgcggc gaactcccac tcggcttcgg
     3421 ttggcaatct gtagcccgcc accgaagagg gatcaaaagt gatgttccca tctgtatcga
     3481 gaaaattgcc attgatatcg taagccagtg gcaattcttc gttttcgctt agccaattac
     3541 agtaagctat tgcgtcccac caactgacat ttattgccgg catgagaccc ctaccccaat
     3601 ctctgtctcc agacctgctc ctgccggtgg cgtcgcaaaa gacatcatat tgatcgaaag
     3661 ttacttcgta cttaccgata aaaaagtcgt aagtgagcgt caccacgtgt gcaggtcttt
     3721 caccatgctc ggcgttctca gaagcatctc ccataatgaa ggtacccctc tcgaccagca
     3781 ccatttcacc tatttcaggt tcggtggcaa agagccagac cgaagttagt agaaaggcta
     3841 taagcaataa cgatattctc aatgcgggat ttcttttcaa atccttccct ccgtccataa
     3901 tgaatgtggt taaacaatac taatatatca tccggtttgc cggaacggca gagttggcaa
     3961 gcctcttctg taaagtgt
//
LOCUS       DAOOLY010000229         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27584_length_3957_cov_3.977960, whole genome shotgun sequence.
ACCESSION   DAOOLY010000229 DAOOLY010000000
VERSION     DAOOLY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27584_length_3957_cov_3.977960"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLD15_10015"
     CDS             <1..880
                     /locus_tag="PLD15_10015"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPB64286.1"
                     /translation="LGGMSHNYYLYDRDGQFVMLPWDFNMAFGGFGGFGGDRGSGGSF
                     YIDTPAVNMASLPIVNRLLTREEYLERYHGYLQLIIDGYLNDEFFAERVEEVAAMIDP
                     YVKDDPTALYSYEQFLASFNSTDGRSTGLIDYNSARVETILKQLSGEIPSKSDGSDST
                     ASSFFERMPAAGNEGWNVPANAMPVRNNRNAENIDPLEMLPEREERVQNMQPPDTQPP
                     QGMPVDAGGMVEGRANMQPPSEMPDFDMGNRARGEAEQIPEKSDDLTRSETITIYASM
                     LALGLSITILKLKRKI"
     gene            1015..1524
                     /locus_tag="PLD15_10020"
     CDS             1015..1524
                     /locus_tag="PLD15_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64287.1"
                     /translation="MKKALLLLILVAVAVSAFGYTFNTNNIGGSIAYFPFILQTFSAR
                     GYYHIGFKLADPWSVGPLEFTQMMLYAGPTVSYSSDLGGASFEFGISGRLFTGIENLS
                     FPFFGRKFMVAVGLRGSSTYSFSKGKFSMPQISPALSIIDVTKMTDRLNYSLYFWPLP
                     VLLGFDVYF"
     gene            1651..1884
                     /locus_tag="PLD15_10025"
     CDS             1651..1884
                     /locus_tag="PLD15_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPB64288.1"
                     /translation="MVVLYKSKYGSCEKYARWIAEDTGADISDISSFDTERLGNYDVV
                     VFGGSLYAVGIIGLSVIDSRTTRKACWHRTQCR"
     gene            1962..2501
                     /locus_tag="PLD15_10030"
     CDS             1962..2501
                     /locus_tag="PLD15_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPB64289.1"
                     /translation="MRVFSVLASPRIKGNTSVVLKRFLEGVRENHSGVEERTAILHEK
                     SIRPCMACDNCKTGPKVCVISDDMQQLYREVEAADVIVLATPIYWWNMTAQLKTFIDR
                     LYAMDFEKSFKGKRLVLLMTYGGEDPNSGAEIVEKSFREICEFLSMEFTGKFGLCSEN
                     PIELRERSLEEAYSLGRKL"
     gene            complement(2545..3714)
                     /locus_tag="PLD15_10035"
     CDS             complement(2545..3714)
                     /locus_tag="PLD15_10035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB64290.1"
                     /translation="MSKLRTFINWIRKERSLNNSIVQKTLETNLRRIYYLALIGIPSH
                     IAHVIMFWITDPSSSEEAVWRTGIIVVHSIHLLVSIAIAIIISIIRKQKHLSKKAVYI
                     QYAFIVSLSAMAISLVTIDQYVTTNITPFLVLCLGLAVIFLLRPSSTLIVFGVVLTAY
                     LLLIGTVQKEPAILLTNRVNGITFVVFGMYLSLILWNTTRKNLFQEEEIKRQQKELEE
                     KNAELQRLAFLDTLTGLYNRRKWYEFAEEESLSICRYSTSGSVVLMDIDHFKKINDDY
                     GHPIGDKLLKEIAGLLRKNLRAVDKPCRWGGEEFIVLLPNTSVEEARKVAEKLRTMIE
                     RTEFSIDGESIRVKASFGIASIRCEENSLDRAYERVDRALYTAKQSGRNIVVSET"
BASE COUNT         1028 a          941 c         1021 g          967 t
ORIGIN      
        1 actgggcgga atgagccaca actactacct ctatgacaga gacggtcaat tcgtgatgct
       61 accatgggat ttcaacatgg ccttcggggg attcggggga ttcggcggcg acagaggaag
      121 cggcggcagc ttctacatag acacaccggc cgtcaacatg gcctcgctcc cgatagtcaa
      181 cagactgctc accagggagg agtacctgga gagatatcac ggctatcttc agcttataat
      241 agacggctat ctgaacgacg aattcttcgc agaacgggtt gaagaagtcg ctgcgatgat
      301 cgatccttac gtcaaggacg atcccacagc gctgtattcg tacgagcaat tcctggcttc
      361 cttcaactcc actgatggcc ggagcacggg actgatcgac tacaacagcg ccagagtcga
      421 aacgatctta aaacaactga gcggggagat tccctctaaa agcgatggat ccgacagtac
      481 ggcgagctct ttcttcgaaa gaatgcccgc ggccggtaac gaaggctgga acgtgccggc
      541 caacgcgatg ccggtgagga acaacaggaa cgcagagaat atcgatcccc tcgagatgct
      601 tcccgaaagg gaggaaagag tgcagaacat gcaaccccca gatacgcaac ctcctcaagg
      661 aatgcccgtc gatgcgggcg gcatggtcga ggggagggca aacatgcaac caccctctga
      721 gatgcccgat ttcgatatgg gaaacagggc gaggggagaa gccgagcaaa tccccgaaaa
      781 gtccgacgat ttgacccgga gtgaaactat aacgatatac gcctccatgc tcgctctggg
      841 attatctatt acgatcctaa agttgaaacg taaaatctag agtgagggac aacccggttt
      901 ttcacttttc cagacaagag gagtgttcct gacttcggcg agcggaaaga ggtttcgagg
      961 cttctggctc cgaaaaggga agaaatgtta tactaaggcc gggaggtgaa aaaaatgaag
     1021 aaagcgttgc ttttactgat tctcgtggct gtagccgttt cggccttcgg ctatactttc
     1081 aacaccaaca acataggtgg gtcgatagcg tattttccgt tcatccttca aaccttctcg
     1141 gccagagggt attatcacat agggttcaaa ttagccgatc cctggagtgt cggtccgctg
     1201 gaattcaccc agatgatgct ttatgccggt ccgacggtct cctacagctc cgacttgggg
     1261 ggagcgagtt ttgaatttgg aataagtgga aggctcttca cgggtatcga gaatctgtcc
     1321 ttccccttct ttggaaggaa gttcatggta gccgtcggat tgcgaggctc ttcaacgtac
     1381 tcgttttcca aaggaaagtt ttctatgccc cagatatctc cggctctgag catcatcgac
     1441 gttacgaaga tgaccgacag gctcaattac tccctgtact tctggcccct gccagtattg
     1501 ctgggtttcg acgtttactt ctgatagatt tgaaataaaa tagtgaaggc ccttaactgg
     1561 gccttttctt cctggagata caggatattc aaatgatgat acaattccct ttgaagaatc
     1621 cggttttctg ggggaattgt tttgtctaaa gtggttgtgc tatacaaatc caaatacggg
     1681 agctgtgaaa aatacgccag atggattgcc gaagataccg gggcggatat ttccgacatc
     1741 tcttcgttcg atactgaaag gcttggcaac tacgatgtgg ttgtcttcgg cgggtcgctt
     1801 tatgctgtgg gtataatcgg tctgtcggtg atcgactcac gaacgacgag aaaggcatgc
     1861 tggcatcgta ctcagtgccg gtgaactgga caaacaggaa gagcacagcg ccgctggtag
     1921 aaagtataga agaattcatc aagaaggggc agtgatcgta agtgagagta ttttcagttc
     1981 ttgcaagtcc gagaatcaag gggaacactt ctgtggttct gaagagattt ctcgaaggag
     2041 tcagggagaa tcacagcggt gtcgaagagc gcaccgcgat tctacacgag aagagcataa
     2101 ggccctgcat ggcctgcgac aattgcaaga ccggtccgaa agtgtgtgtc ataagcgacg
     2161 acatgcagca gctttacaga gaggttgagg ccgcggacgt tatagtgctc gctaccccca
     2221 tctactggtg gaacatgacc gctcagctga aaaccttcat cgacaggctt tacgcgatgg
     2281 actttgaaaa atccttcaaa ggcaagagat tggtcctcct gatgacttac ggcggtgagg
     2341 atcccaactc cggcgccgag atagttgaaa agagctttag agagatctgc gagtttctct
     2401 ccatggagtt caccggtaag ttcgggctgt gcagcgaaaa tcctatcgag cttagagagc
     2461 gctccctcga ggaagcttac agtcttggca gaaagttgta accgactcgg acgtaccagg
     2521 aaactcaaca agtcgaaaat cggctcacgt ttcggacacg actatgtttc ggccgctttg
     2581 tttggccgta tatagagccc tgtccacgcg ctcataagct ctatccaggg aattttcctc
     2641 acatcttata gacgcgatcc cgaaactcgc cttcactctt atgctttctc catcgatcga
     2701 aaactcggtc cgctcgatca tagttctgag tttttcggcc acctttctag cctcttcgac
     2761 agatgtgttg ggaagcaaaa cgataaactc ttctccgccc caacgacagg gtttatcgac
     2821 ggcgcggaga tttttcctca acaggcctgc gatttccttc agaagcttgt ccccgatcgg
     2881 atggccgtag tcgtcgttga tttttttgaa gtgatcgatg tccatgagca ccacactgcc
     2941 gctggtactg tatctgcaga tcgataggct ctcttcctcg gcaaattcat accattttct
     3001 cctgttgtaa agaccggtga gggtatcgag aaacgccagt cgctggagct cggcgttctt
     3061 ttcttcgagt tctttctgct gtcttttgat ctcctcttcc tggaagagat tctttcgggt
     3121 tgtattccac agaataagcg aaaggtacat tccgaatacg acgaaggtga taccgttcac
     3181 tctgttggtc aaaagtatgg cgggctcctt ctggacagta ccgatcaaaa gcaggtaagc
     3241 cgtcagaaca actccgaaga ctattaaagt ggatgatggt ctcaaaagaa agataaccgc
     3301 caatcccaaa cagagaacaa gaaaaggcgt tatgttggtg gtcacgtact ggtcgatggt
     3361 caccagagat atcgccatgg ctgaaagtga aacaataaaa gcgtactgga tatagacagc
     3421 ctttttagat agatgctttt gctttctgat tatggaaata attattgcaa tagcgatact
     3481 gactagcaga tgaattgaat ggacgactat gatacccgtt cgccaaacag cctcttcgct
     3541 ggaggaagga tctgtaatcc agaacatgat cacatgcgct atatgagacg gaatacctat
     3601 cagggccaga tagtagatcc tgcgcaggtt cgtctccaga gtcttttgga ctatggaatt
     3661 gttcagcgat ctttcctttc taatccagtt tatgaaagtt cttaatttgc tcacgctaga
     3721 gtccatactt ctcctgaaat aatcctctgt atattctacc acggtatatt ctaccaccgg
     3781 ctaacggcaa tttcaccagt ttatagtgca acttcgtgcc ataaaaccgt gcgatatgcc
     3841 gaagagaaga gcggaagagc cgtccttggt ccttcgtcca ggagcgtgaa cccgtccttg
     3901 gggaacctga ggctagaggt aagaggcgag aggctggaag agcgggaaga cccgtcc
//
LOCUS       DAOOLY010000230         3938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27770_length_3938_cov_3.893639, whole genome shotgun sequence.
ACCESSION   DAOOLY010000230 DAOOLY010000000
VERSION     DAOOLY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27770_length_3938_cov_3.893639"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..503
                     /locus_tag="PLD15_10040"
     CDS             24..503
                     /locus_tag="PLD15_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64291.1"
                     /translation="MPEERVSTKSSIAQVFMGVIVIALGLSIIFVPSLLENFWLLFIW
                     VPGLIMEYNAIRGEKGLFVPGGVLLTVALALTLGVIFDGFYEGGGWALFILAPAVGLL
                     QLYLAGGRKPKALLTPIFVLTIVAVISLIPSTLSKWAVIIVGALLIGFGVLILLKKK"
     gene            complement(504..1031)
                     /locus_tag="PLD15_10045"
     CDS             complement(504..1031)
                     /locus_tag="PLD15_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB64292.1"
                     /translation="MEEVYRSIEGLEIVRICEEDAQKVIEYVNRVSGESDYLSFGENE
                     FMTTVEEEREVIRSYRERDNCLIIAARLGGNIVGLLTFEGGRRERLKHRGEFGLTVSK
                     EYWGMGIGKRLIEYMIKAVKESGITRIGLQVRVDNVRAIKLYSNLGFEIEGRIKKFMK
                     IKEKYFDFLQMGFDL"
     gene            complement(1043..1396)
                     /locus_tag="PLD15_10050"
     CDS             complement(1043..1396)
                     /locus_tag="PLD15_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64293.1"
                     /translation="MEEKTIEGILKLKEEQINSMDFKKLLAYIRSIDEYFRSNTNPDV
                     ETALKTYQKAIQLLTAARMRLMVLKKEKEELDRKYSQFLASISGETIGESNSGETEED
                     EEKSSDTQKNGRLPF"
     gene            complement(1398..2936)
                     /gene="xseA"
                     /locus_tag="PLD15_10055"
     CDS             complement(1398..2936)
                     /gene="xseA"
                     /locus_tag="PLD15_10055"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPB64294.1"
                     /translation="MEELTIEFKDIENMLEHIRSVLVRVGLNNLRVKFPADVTNVGAK
                     GNYVYINVSQDYTERGAKRKIDLNMIADMAAFGRMGRQLGLKSSNELKGKKWQFQGTI
                     SFYKPRASFSIWIDTIAPLGESDITVRRREIYAALKSNNALRTEEHELSELQPIRKIA
                     VISSATAAGLGDFYSNLNLKEPYRPVVHLYESFMQGSETVPGILKALNMIKTSLIAYD
                     VVVITRGGGSAIDLMYFDDLDLGMAISAFSRDYCPILSAIGHERDFTIPDFVSWKRFD
                     TPTAAAKAITEQINTHSESLNQAAATLKKEMEWLFEKTSTLAEFERLREFATRVETLI
                     ERSDEQFETVAERTSRRLLSEIERAASFIDSLDRDSYAITIERTFERVLILTDSLFER
                     ASTILDRHLKESASALDKVNVDVSMDSLLFRTDTSLDLFEKGVLRSFERVESNAVNNL
                     EELSSGLVEHGGYAASLLLGGVVLKRDSHVVRSAREVTRGDRLECYFADGCATTIIDE
                     VTEV"
     gene            complement(3029..3406)
                     /locus_tag="PLD15_10060"
     CDS             complement(3029..3406)
                     /locus_tag="PLD15_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64295.1"
                     /translation="MKRNIIRLALVIVLIVLGLCLYVLGKEHKIFVDNRDIVINGTEL
                     KSTNPFIVYVDNVDLEEVGKGKRKVAYSPGPWHTVKAIEKLPDGTTREFVKKFTLSPK
                     DTAIINLPALVSEKDGWLKIEGE"
     gene            complement(3409..>3938)
                     /locus_tag="PLD15_10065"
     CDS             complement(3409..>3938)
                     /locus_tag="PLD15_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64296.1"
                     /translation="IIIGLCIFVYWFTKTKLGQDMRAVGQDMQVAATAGINVDRTRTL
                     SIIISTVLACFGQIIFLQNIGTINTYNSHDQVGTFAIAALLIGGASVVKATIPNVFIG
                     ITLFHLTFIVAPRAGKELLGQAQIGEFFRVFVSYGIIAISLALYAWRRQVEKETERRQ
                     AKAAIKAAIEDKAEG"
BASE COUNT          902 a         1007 c          855 g         1174 t
ORIGIN      
        1 agttgaatgg aggaggtgta atcatgcccg aagaaagagt ttcaaccaaa agcagtattg
       61 cccaggtctt catgggcgtt atagttattg ccctgggact gtcgattatt ttcgtccctt
      121 cattgctgga gaatttctgg ttattgttca tatgggttcc cggactgata atggaataca
      181 acgccatcag aggggagaag gggcttttcg ttccaggagg agtgttgctt accgtcgctc
      241 ttgcactcac tctgggagtg atcttcgatg gtttctacga aggtggaggc tgggcccttt
      301 tcatacttgc tcctgccgtt ggtctcttgc agctgtacct ggccggtgga agaaaaccta
      361 aagcacttct gacaccgata tttgtactaa ccattgttgc agtgatatcg ctgataccct
      421 ctactctgtc caaatgggcg gttataattg tcggtgccct tctgatcggg tttggagtgt
      481 tgattcttct caaaaagaag tgatcaaagg tcgaaaccca tctgtagaaa atcgaagtac
      541 ttttctttta ttttcatgaa tttcttgata cggccttcga tctcgaaccc aagattcgaa
      601 taaagcttta tagctcgtac gttatcgact ctaacttgaa ggccgattct tgtgatgccg
      661 ctctctttta ccgctttgat catgtattcg ataagccttt ttcctatacc cattccccag
      721 tattctttag agactgtaag accgaactca cctctgtgct tcagcctttc tctgcgtcca
      781 ccttcgaagg tcagcaatcc cactatattc cccccgagcc gggcagcgat tatcaggcag
      841 ttatcgcgtt ctctgtaaga tctgatgact tctctctcct cttcgacagt agtcatgaac
      901 tcattttcac cgaaactcag gtagtcgctc tctccgctca cccggttgac gtattcaatg
      961 actttctgcg catcttcttc acagatccgg acgatctcta aaccctctat acttctgtaa
     1021 acctcctcca ctgcactcgc acctaaaaag gaagcctgcc attcttctgg gtgtcgctcg
     1081 atttctcttc atcttcttct gtttcgccgg agttcgattc cccgatcgtt tccccggata
     1141 tggaggcgag aaactgagag tattttctat cgagttcctc tttctctttc ttcaacacca
     1201 ttagcctcat tctggcagct gtcagtagct gaatcgcttt ctgataggtc ttgagggccg
     1261 tttcaacatc gggattggta ttactcctga agtattcgtc gatcgatctg atatatgcaa
     1321 gaagcttttt gaaatccatg gagtttatct gttcttcttt gagcttcagt atcccctcta
     1381 tcgtcttctc ttccatctca tacctccgta acttcatcta taatggttgt cgcgcaccca
     1441 tcagcgaaat agcattcaag cctatcgcct ctggtcactt ccctggcgct tctaacaacg
     1501 tgagaatctc ttttcaaaac aactccgcct aaaagtagag atgcggcata gccgccgtgt
     1561 tccaccaacc ccgaagatag ctcctcaaga ttatttactg cgttcgattc aactctttcg
     1621 aacgatctca gcacaccttt ctcgaaaaga tccagagagg tgtctgtgcg gaagagaaga
     1681 ctgtccatag acacatcaac atttacctta tcgagtgcac tcgcggattc tttcagatgt
     1741 ctgtccagaa ttgtgctggc tctctcgaag aggctgtctg taagaattaa aactctctca
     1801 aaggtcctct cgattgtgat ggcgtaactg tccctgtcga gggagtctat gaaggaggct
     1861 gctctttcga tttccgacag tagacgtctc gatgttcttt cagcaactgt ttcgaactgt
     1921 tcgtcggatc tctcaatgag tgtctccact ctggtggcga actctctgag cctttcaaac
     1981 tccgctaacg tggatgtttt ctcgaagagc cattccattt cttttttcaa cgttgcggcc
     2041 gcttgattca ggctctcgct gtgagtgttt atctgttcgg ttatggcttt cgccgcagcg
     2101 gtgggagtat cgaaacgctt ccaggagacg aaatccggta tggtgaaatc acgttcgtgg
     2161 ccgatagcgc tcaagatcgg gcagtaatct ctgctaaaag ccgatatagc cattcctaaa
     2221 tctagatcgt cgaaatacat aagatcgatc gccgaacctc ccccgcgtgt aataactacc
     2281 acatcgtaag cgataagaga ggtcttgatc atgttgagag ccttcaagat ccccggaacg
     2341 gtttcgctgc cctgcatgaa agattcatat agatgaacca cgggtctgta cggctcttta
     2401 agattcaaat tggagtagaa atcccccagc cctgcggctg tggccgacga gatgacggct
     2461 attttgcgta taggctggag ctcgctcaat tcgtgttcct ctgttctgag tgcgttattc
     2521 gatttcaacg cagcgtagat ctcgcgacgc cggaccgtaa tatccgactc gcctaatggt
     2581 gcgatggtat ctatccagat ggagaaactg gcccggggtt tgtaaaagct gatagtaccc
     2641 tgaaattgcc acttttttcc tttaagttcg ttagatgatt tcaatccgag ctgtcttccc
     2701 attctaccga aagccgccat atccgcgatc atgttcaaat cgatctttct ctttgcacca
     2761 cgctccgtgt agtcctgtga aacgtttata tagacgtagt ttcccttcgc tccgacgttg
     2821 gtgacatcgg ccgggaattt gactcttaga ttgttgagac cgacccgaac taatacgctc
     2881 ctgatatgtt cgagcatgtt ttcgatatct ttgaattcga tggttaactc ttccatctca
     2941 acttcctccg gaaagcagtc tagtcgtcct taaaaatgat aacagaatag cgtttgaaat
     3001 gcggtgggag ttatagaaaa gagggcgatt actcgccctc aattttcagc cacccatcct
     3061 tttcagatac cagggcaggt aagttaataa tagcagtgtc tttcggtgat aaagtgaatt
     3121 ttttgacgaa ttctcgcgtg gttccatcgg gaagtttctc gatcgctttc acagtatgcc
     3181 atggtcccgg cgaataagcg acttttctct tgcccttacc aacttcttcg agatcgacat
     3241 tgtcaacgta cacgatgaaa gggttggttg acttcagctc ggttccgttt atcacgatat
     3301 ccctgttgtc cacgaatatc ttgtgctctt tccccagaac atacagacac aatcccagga
     3361 ctatcaacac aatcacgagg gcgagcctga ttatatttct cttcatcatc atccctccgc
     3421 cttatcttct atggccgcct tgatcgccgc ttttgcctgc cttctttccg tctctttttc
     3481 tacctgtctt ctccaggcgt aaagcgcaag cgatatcgcg atgattccgt aagagacgaa
     3541 gaccctgaag aattctccga tttgagcctg acccagaagc tccttgccgg ctctcggagc
     3601 gactataaaa gtcaggtgga agagcgttat accgataaac acattcggta tcgtagcctt
     3661 cacaaccgac gcccctccta tcagcaaggc agcaatcgcg aaggttccaa cctggtcgtg
     3721 agagttgtaa gtgtttatcg tccctatgtt ctgcaggaag attatctggc cgaaacatgc
     3781 gagtacggtc gatattatta tcgacagcgt cctggttcgg tcaacgttta tacctgctgt
     3841 ggcggcaact tgcatatcct gcccgacagc cctcatgtcc tgcccgagtt tggtcttggt
     3901 gaaccaataa acgaagatgc acaaaccgat tattatcg
//
LOCUS       DAOOLY010000231         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27854_length_3930_cov_3.820903, whole genome shotgun sequence.
ACCESSION   DAOOLY010000231 DAOOLY010000000
VERSION     DAOOLY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27854_length_3930_cov_3.820903"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLD15_10070"
     CDS             <1..1000
                     /locus_tag="PLD15_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB64297.1"
                     /translation="SVVTIKDVAAEAEVSIATVSRVLNGSGYVSEDTKVRVLRVINRL
                     NYHPMPSIRKRSLYRTLGVLLPNTMGNHYGEIFMGIEEYAHRNGFNVMLAMAREMMSR
                     EQEILNEYFQRKVDGVIVATLHSDEHMLNMFIQSGIAVVAVDSPIREIRADSVNIDNA
                     SAAYCVAKYLYDRGHRHVLFLPGQEDVHASRDRLKGLRKFATRSPDFNLKVARIGGFE
                     PHHGQEAIRDYLLNPGVNFTAIFAVNDHVAMGAIQELHRNHIRVPEDVSVIGFDDSVH
                     APFTIPSLTTVSQPRIEMGSAAAQLLIERLKSTQKRVFRNIVLPTAIIERESVKIV"
     gene            1027..2007
                     /locus_tag="PLD15_10075"
     CDS             1027..2007
                     /locus_tag="PLD15_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPB64298.1"
                     /translation="MGITIIGHRLAGLPWEERPGNDDPIWRYTKNPIITREAVKGVNS
                     IFNSAVVVCEDGYRGIFRADTKELIMQLHAGRSQDGLSWKIDQNRIEFQSNEPEISRF
                     IYGYDPRVVWIEDRYYITWCNGYHGPTIGIGYTYDFTNFNQLENAFLPFNRNGVLFPR
                     KINGKFAMLSRPSDNGHTPFGDIFYSESPDMVHWGCHRHVMSPTTGWQSTKVGAGPVP
                     IETSQGWLLVYHGVWTSCNGFVYSAGVALLDLERPWKVIKRSRHYILNPRMPYENVGD
                     VPNVIFPCASIQDADTGRIAIYYGAADTVTALAFTTAEVLYDFLEEFSYR"
     gene            2174..3175
                     /locus_tag="PLD15_10080"
     CDS             2174..3175
                     /locus_tag="PLD15_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPB64299.1"
                     /translation="MERIAWIDISRGFLMSLVVLFHTLPPQLVADLINPAACTFFFLS
                     GLISRDAPILFTIKKRFKQLMVPYYFMALVNLGIWILAWAISKRTELDLPVIDVVWNI
                     LLVRTALGIMPFNIIPLWFLPAVFVMEIYYIVFKRLKILTIGILAGFVSMFFFRGSLP
                     FKIDVALAVMPYFALGKAMQEKKDRISTIGWPVVVVAIAIFTSSSFLCGEVYLMEDYF
                     GPQPLIYIVAALSGIVSIIGASRMIEKFFKIADFFSRIGRNTLFVLGYHIAAGVLVYP
                     IFDLFGDPIEILRKFWILYWFINILVVYLAIRFLPTRVISFLSGIFPPHIKRDLNTR"
     gene            3396..>3930
                     /locus_tag="PLD15_10085"
     CDS             3396..>3930
                     /locus_tag="PLD15_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64300.1"
                     /translation="MLVISIIALIFLGYLCYRLIKREGGIFLGPYEFKFTREPGPEEY
                     MKRYKELQKKNQEFESRLVLSAAANRFPQNADIFKTLMEKIFADLKVAKSEKDIEDIM
                     VRGERALEELGRNAGSDSMVLVEQYSKKLLEIREEFEQLKARREDEIKQQQIEKNREV
                     LLELESILEGIKASDDEM"
BASE COUNT         1041 a          893 c          954 g         1042 t
ORIGIN      
        1 ttcagtggtt acgatcaaag atgtcgctgc cgaggcggaa gtttccatag caaccgtttc
       61 ccgtgttttg aatggaagcg gctacgtttc cgaggatacc aaggtacgcg tattgagagt
      121 gataaacaga ctcaactacc acccgatgcc ctcgatccgc aagcgcagtt tgtacagaac
      181 tctgggagtg ctgttaccca acaccatggg caatcactac ggcgaaattt tcatgggtat
      241 cgaagagtac gcccacagga atggtttcaa cgttatgcta gccatggcca gagagatgat
      301 gagccgcgag caagaaatcc tcaacgaata cttccagaga aaggtagatg gcgtgatagt
      361 cgcaaccctt cacagcgacg aacatatgtt gaatatgttt atacagagcg gtatcgctgt
      421 tgtggccgtt gattctccca tcagggaaat ccgggccgat tccgttaaca tagacaacgc
      481 atcggctgcg tactgtgtgg cgaagtacct ttacgacagg ggtcacagac acgttctctt
      541 cctccccggt caggaggatg tgcacgcttc cagggacaga cttaaaggac taagaaagtt
      601 cgctaccaga tctccggact tcaatctaaa ggtggctaga ataggtggtt tcgaacctca
      661 tcatggacag gaggctatcc gtgattatct cctaaatccg ggagtgaact tcacggccat
      721 cttcgctgtc aacgaccatg ttgcaatggg agctatccag gaactgcaca gaaaccatat
      781 ccgcgtaccg gaagatgtat ctgtgatcgg tttcgacgat tctgtccacg cgccgtttac
      841 gatcccctca ctcactactg tctcacaacc gagaatagag atgggaagcg cggccgccca
      901 gttgcttata gagaggttga aatccacaca gaagagggtg ttcaggaaca ttgtgttgcc
      961 aaccgcgatt atagaaagag aatccgttaa gattgtatag aagaggatcg aagggagagt
     1021 gtttccttgg gaatcactat aattggccat agacttgcag gtttgccctg ggaggagagg
     1081 cctggcaacg acgacccgat ctggagatat acgaaaaatc ccattatcac gcgtgaagcc
     1141 gtgaaaggcg tcaacagcat attcaacagc gcggtggtag tctgtgaaga cgggtacagg
     1201 ggaatctttc gtgcagatac aaaagaactg ataatgcagc tccatgccgg gaggagccag
     1261 gatgggcttt cgtggaaaat agatcagaat cgcatcgagt ttcagtccaa cgaaccggag
     1321 ataagccgtt tcatctacgg ttacgatccc agagtggtct ggattgaaga ccggtattac
     1381 attacctggt gtaacggcta ccatggaccg acgatcggta tcggctacac ctacgatttc
     1441 acgaatttca atcagctcga aaacgccttt cttcccttca accggaacgg tgttctcttt
     1501 ccgagaaaga taaatggcaa attcgccatg ctcagccggc ccagcgacaa cggccatact
     1561 cccttcggtg atatcttcta cagcgaaagt ccagacatgg ttcattgggg atgtcatagg
     1621 cacgtcatgt ctcccaccac cggttggcaa tccacaaagg tcggagccgg gccagttccg
     1681 atagaaacct cgcaaggctg gttgttggtt tatcacggtg tctggacctc ctgcaacggt
     1741 tttgtttata gtgccggagt agccttgctc gatctggaga gaccctggaa ggtgataaag
     1801 aggtccaggc actacattct caatccacgt atgccttacg agaacgttgg cgacgtacct
     1861 aatgtgatct tcccgtgtgc cagtattcaa gatgccgata caggtagaat cgcgatatat
     1921 tacggcgcgg ccgacacggt aacggccctc gcttttacaa cggctgaagt gttgtacgac
     1981 tttctggagg aattttcgta caggtaagaa atcggataat tttcgttaga tgcaatttta
     2041 agttttgctc ttcagacaaa gacctgcgat cacactaatc cgtcgtacca ttccgttatg
     2101 ctatactgat gtcaattatc ataacgggtg gttgttctgt atgcaagccc cagatgcgtc
     2161 atccacaatc aaaatggaaa gaattgcctg gatagatatt tctaggggtt ttttgatgtc
     2221 tctggtggtt ctctttcaca cactgcctcc gcagctggtt gcagacctca taaatcctgc
     2281 cgcatgtacc tttttctttc tttctgggct gatctcaaga gacgcaccga ttcttttcac
     2341 aatcaaaaag aggtttaagc agctgatggt tccctactat tttatggctc tggtgaattt
     2401 gggaatttgg atactggcct gggccatttc taagcggact gaactggatt tgcctgttat
     2461 agacgttgtg tggaacattt tgctcgtaag gacggcactt ggtattatgc ccttcaatat
     2521 catacctctc tggtttttgc ccgcggtctt tgttatggag atatattaca tcgtgttcaa
     2581 gagattgaag atacttacga taggtattct tgcggggttc gtttcgatgt tctttttcag
     2641 aggctctttg cctttcaaaa tcgacgtagc tctggccgtt atgccttatt tcgcactcgg
     2701 aaaggccatg caagaaaaga aagataggat ctctacaatt ggctggcccg ttgtggtagt
     2761 ggcgatcgcg atttttacgt catcgtcttt cctttgcggc gaagtctatc tgatggaaga
     2821 ttactttggt ccccagccgt tgatctacat agtcgccgca ctttccggta ttgtctctat
     2881 catcggcgct tcaagaatga tcgagaaatt tttcaaaata gcggacttct tttctagaat
     2941 aggacggaac acacttttcg tgctcggcta tcatatcgcc gcaggagtac ttgtctatcc
     3001 tatttttgat ctttttggag atccgataga aatcctcaga aagttttgga ttctgtactg
     3061 gttcatcaat atattggttg tttatctggc tataaggttt ttaccgacac gcgtgatttc
     3121 cttcctatct ggaatttttc ctcctcacat caaacgagat ctcaacaccc ggtaaccctg
     3181 cgccgacaaa cggagacggc gcttcccaac aatcggtcga cccgtgttat cattttattc
     3241 gtaaggattc tcgaagagtt agtatttcac gcaaatattt ttcgaccgga aactgaattt
     3301 aggtgaatcg agttctatac tattatctat gaaaactaga acttcatata ttgcaagtac
     3361 aagctccatt cgaatgttcg caaggaggga ccgtaatgct ggtaatatct ataattgctc
     3421 tcatctttct gggttatctc tgctacaggc taatcaaaag agagggtggt atctttttgg
     3481 gaccctacga attcaaattc accagagaac ccggccccga agagtatatg aagcgttaca
     3541 aggaactcca gaagaaaaac caggaattcg agagtagact ggtgcttagc gctgcggcca
     3601 accgttttcc gcagaatgcc gatatcttca agactcttat ggaaaagata ttcgcggact
     3661 tgaaagtcgc aaaaagtgaa aaagatatcg aagatatcat ggtccggggt gaaagagctc
     3721 ttgaagaact cggcagaaat gccggaagcg attccatggt tttggttgaa caatattcga
     3781 agaaactgct cgaaatcagg gaggaattcg aacaactaaa ggctcggcgc gaagacgaaa
     3841 tcaagcaaca acagatcgaa aaaaacagag aagtgctgct ggaactcgag agcattctcg
     3901 aaggtatcaa ggcctccgac gatgaaatgg
//
LOCUS       DAOOLY010000232         3917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_27980_length_3917_cov_4.642413, whole genome shotgun sequence.
ACCESSION   DAOOLY010000232 DAOOLY010000000
VERSION     DAOOLY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27980_length_3917_cov_4.642413"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..510
                     /locus_tag="PLD15_10090"
     CDS             196..510
                     /locus_tag="PLD15_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64301.1"
                     /translation="MSPALSLSGRAGGFSFVEVLIALLIIIISVVALLNFLTTSLRVN
                     DTSSKIAGDLLVSSGELEIGDINGETATVTVNGVKISGKLRESPYGSSKKLVEFVRPS
                     GD"
     gene            503..1039
                     /locus_tag="PLD15_10095"
     CDS             503..1039
                     /locus_tag="PLD15_10095"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB64302.1"
                     /translation="MTERKGKPGLTLMEVLIAMAIIVAVLIISFNIYNLWWKAYNLAS
                     SKAVIERGLTSAIEIIIKELRVAKSVALVEDFDSLSPTPSSTNLFIGLDQNEKRVKIW
                     SGDKNNLSNLIDEDIDLLAFSVDDEDKYLLNIRIGNTEKSVELSSSVKILNKNLKKDQ
                     SYTETSIVGFEKSVKLFE"
     gene            1130..1951
                     /locus_tag="PLD15_10100"
     CDS             1130..1951
                     /locus_tag="PLD15_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64303.1"
                     /translation="MKRYFSAILALFLVAVLITGCFQLPKAPDSIKTRVNNVIEKWNN
                     LYANELEIISWNYEGILDDPVKYQGVLALLTDPDSVRIVHTDITDPGLLSLLLLSAVP
                     ADSQKEFKESVEGLVEIGQHLVKLKWKAPASGEKFVTTALVEEDNIVWDSILGSVVEE
                     EFEITPAGGKSGEVTIKWFWGAERGSISWSLECSGPPLQCSYDSKAKMTSGEARIETR
                     LVKTEECCTLYYGWAYRTPTGSISIDWNPETNQYDIEVSSWGSTGSGQGILECCM"
     gene            complement(1995..2564)
                     /locus_tag="PLD15_10105"
     CDS             complement(1995..2564)
                     /locus_tag="PLD15_10105"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB64304.1"
                     /translation="MKEDKAQSQDRKAKEIHPDTKAIIEHYRFQRLPVESTLYVSTYR
                     SGAKTLEGGPLSTAMIGLYSDSPESLSLFHRLDIDEVWHFYGGDPLKLVLLYPDGSSE
                     DITMGNDFGSGQLVQFVVPAGVWQAGYMVPGGRYSLFGCTMAPGFTASCFEAGTADEL
                     LKDYPQRSEEIIRLSISGNREKMPDGFSG"
     gene            complement(2594..3685)
                     /locus_tag="PLD15_10110"
     CDS             complement(2594..3685)
                     /locus_tag="PLD15_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB64305.1"
                     /translation="MEKIRAAIVGTGFAAEIHARALSELGYKVAAVVGSNLSRTSAFA
                     EKWKIERYGVDFELALQEDIDCVHICTPPALHYVMAKRAMESGKHVICEKPLCLDTAE
                     AEKLLTLSKEKGLVAAVNFNVRFNGKCHEARKLVAASEFGGVCLIHGAYLQEFQALPD
                     RYSWRYKPELAGHMRAVTEIGSHWIDLARYLTGLEVTEVSADFGAFTPQRYLSGDDMY
                     LEYREGSERITVNSEDAAAILLKFDSGAIGNVILSEVSHGRGNRLEIEVTGRNESVWW
                     DSESPCELNRSRKSGGVLTSSDAFCGGFVDTFKDCFREVYADIERGGPSVKPSYPTFE
                     DGYRNTAVCSAIYESAVKGRWMQVEGNPR"
BASE COUNT          915 a         1056 c         1042 g          904 t
ORIGIN      
        1 accaaattcg actgtaaata tgagtggaac ttcggccttc tacggtgcga tagttgccaa
       61 ggagctcact acttccggtg gtggaagttc cggaggaatc gtatacatga tcgatggcct
      121 cggcgacact ctcgatccga tcgtggaagt caacgccagc gaactgtggg gcgagtgagg
      181 gcggtgattc accgtgtgag tccggcactt tcgctttccg gtcgagccgg aggcttctcg
      241 ttcgtggagg ttctcatagc tctgctcata attatcatct cggtggtagc acttctcaat
      301 tttcttacca catctttgag agtgaacgac acctcttcca aaatcgcggg agatctcctc
      361 gtcagctccg gagagcttga aatcggagat ataaacggag aaaccgcgac ggtcaccgtg
      421 aacggcgtca aaatctccgg taagttgcgc gagtccccct acggctcgag caagaaactc
      481 gtagagtttg tgaggccttc cggtgactga acgcaaagga aagcccggcc tgaccctcat
      541 ggaagtcctg atagccatgg cgataatagt ggccgttctc ataatctctt tcaacatcta
      601 caacctctgg tggaaggcct ataacctggc ctcaagtaag gccgttatcg agagagggct
      661 gacctcggcg atagagataa taatcaaaga gttgagagtg gcgaagagcg tggcactcgt
      721 ggaagatttc gattcgctct ccccgactcc gtcttctacg aacctgttca tcgggctgga
      781 tcagaacgag aaaagggtga agatctggtc gggcgacaag aacaaccttt ccaatctgat
      841 cgacgaagat atcgatctgc tggccttcag cgttgatgat gaagataaat atttactgaa
      901 cataaggatt ggaaacaccg aaaagagcgt cgagctatct tccagcgtca agatactcaa
      961 caaaaacctc aaaaaggatc aatcttacac ggagacctcg atcgtcggct tcgaaaagtc
     1021 agtgaaactc ttcgaataaa gacctcttct caagatccat tgtcatgacc ggagaaaatg
     1081 ctattctcta tatacgagat atattctcgg aatataaggg gtgataacaa tgaagagata
     1141 tttctctgca attcttgccc ttttccttgt cgcagtcctc ataacgggtt gctttcaact
     1201 gcctaaagct cccgacagta taaagacccg tgtgaacaac gtgatcgaaa aatggaacaa
     1261 cctctatgca aacgaactgg agataatctc ctggaactac gaaggcattc tggacgaccc
     1321 ggtgaagtat cagggggttc tcgcgctcct gaccgatccc gactcggtaa gaatcgtgca
     1381 caccgatata accgatcccg gccttctgag tctgcttctt ctctcggcgg ttccggccga
     1441 tagtcagaag gagttcaagg agtccgttga agggcttgtc gagataggcc agcatctggt
     1501 gaaactgaaa tggaaagcac cggcctcggg tgagaaattc gtcactaccg ctctggtcga
     1561 ggaagacaat atcgtctggg attcgatact ggggagcgtc gttgaggagg aatttgaaat
     1621 aacgccggcc ggcggaaagt ccggagaggt tactatcaaa tggttctggg gtgccgaacg
     1681 cggctcgatt tcctggagcc tcgaatgctc cgggccgccc ctccagtgca gttacgattc
     1741 gaaagcgaag atgacctcgg gcgaggcgcg catagaaacc aggctggtaa agacggagga
     1801 gtgctgcacg ctctactacg gctgggccta cagaacgccc accggctcga taagcatcga
     1861 ctggaatcct gaaaccaacc agtacgatat agaggtgagc agctggggaa gcaccggaag
     1921 cggtcaggga attctggagt gctgcatgta gtttttgtcg gcagctttcc gtgtatgaaa
     1981 ctgccggagg attctcaacc ggagaacccg tcgggcatct tctcccgatt gccgcttatg
     2041 ctgagcctga ttatctcttc cgaccgttga gggtagtcct tgagcagttc atcggcggtt
     2101 ccggcttcga agcaggaggc ggtgaatccc ggcgccatcg tgcagccgaa aagcgagtaa
     2161 cgtccccccg gaaccatata accggcctgc cagactccgg ccggaacgac gaactgaacg
     2221 agctgtccgc tgccgaagtc gtttcccatc gttatatctt cgctcgagcc gtcgggataa
     2281 agcagaacca gtttcagggg atcgcctccg tagaaatgcc acacctcgtc gatatctaga
     2341 cggtggaaca gtgaaagact ctccggactg tccgaataga gcccgatcat ggccgtcgag
     2401 agcggcccgc cttcgagcgt tttggcgccg gaacgatagg tgctgacata cagggtgctc
     2461 tcgaccggca gccgctggaa ccggtaatgc tctattatcg ctttcgtatc cgggtgaatc
     2521 tctttcgctt ttctatcctg tgattgagcc ttatcttcct tcatcgtgcc tcctcgagcg
     2581 cagctgtctt gactcatctg gggttgcctt ctacctgcat ccatcgacct tttacggcgc
     2641 tttcatagat ggccgagcag acggcggtgt ttctgtaacc atcttcgaaa gtggggtatg
     2701 aaggtttgac agaggggccg cccctttcga tatcggcata aacctctctg aagcagtcct
     2761 tgaaagtatc gacaaaacct ccgcagaaag cgtcggaact ggtgaggaca ccgccggatt
     2821 tgcggcttct gttgagctcg caagggcttt ccgagtccca ccacaccgac tcgttccgtc
     2881 ctgttacctc tatctcgagc ctgtttcccc ttccgtgaga aacctccgac agtataacgt
     2941 tcccgatagc accgctgtcg aacttcaaaa ggatcgccgc cgcatcctcc gaattgaccg
     3001 tgatcctctc cgaaccctct ctgtattcga gatacatatc gtctcccgag agatatcttt
     3061 gcggggtgaa cgcgccgaaa tcggccgata cttcggtcac ttcgaggccg gtcaggtatc
     3121 tggcgagatc gatccagtgg gaacctattt cggtgacagc gcgcatatgt ccggccagct
     3181 cgggtttgta gcgccaggag taacggtccg gaagagcctg aaattcctgg agataggcgc
     3241 cgtggatcag acagacgccg ccgaactcac tggcggctac gagcttcctg gcttcgtggc
     3301 actttccgtt gaatcgtacg ttgaagttga cggccgcgac cagccccttt tccttcgaga
     3361 gggtcagaag tttttcggct tcggccgtat caagacagag gggcttctcg catataacgt
     3421 gcttccccga ttccatagcc cgtttcgcca tcacgtaatg aagggccggg ggcgtgcaaa
     3481 tgtgtacgca gtcgatatct tcttggagcg cgagttcgaa atcgactccg taacgctcta
     3541 tcttccactt ctccgcgaag gccgatgtac gcgataggtt cgacccgacc actgcggcca
     3601 ccttgtatcc gagctcactt agcgcccggg cgtggatctc cgccgcgaat cccgttccta
     3661 caattgccgc gcgtatcttc tccatatctc cctcgaaaaa cgtattgagc gccgtcatct
     3721 ccggccggtg cagccggttg acactcgccg tgcatctcta ttttgccaca caattggaaa
     3781 agttgacaca gccagaaatc ggccgtttgc gcagaccggc ccgcattcta agaaccgttg
     3841 ttggtaaaaa catgagaaag agcgtattcg agagttcgct tcgctcacga gaaggcgaga
     3901 attctccttc ataaccg
//
LOCUS       DAOOLY010000233         3885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_28280_length_3885_cov_5.110966, whole genome shotgun sequence.
ACCESSION   DAOOLY010000233 DAOOLY010000000
VERSION     DAOOLY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28280_length_3885_cov_5.110966"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1133
                     /locus_tag="PLD15_10115"
     CDS             207..1133
                     /locus_tag="PLD15_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB64306.1"
                     /translation="MAENSGSKNVAYFAGIGSSVIFGLSFLFTKSALEYTNPVYFLSY
                     RFFIAFITINLLRVFGAIRIRPYRQVFKELFFLGLVQPVLYFILENAGVSLSTSSEAG
                     ILLSTIPIFVMILARIFLKEKIALPRVLSILTALAGILLITAGKGLSSGGTVLGPVLL
                     LGSTLTAAMYNILSRKASAKFTPWEITYHMMTAGFSTFFTMALVLSLLRGEPFEYLSQ
                     FLVPSVFNAALYLGTLSSVGAFFLINLMLSKLEASRATVFAYLSTVVSLLAGIIVRGE
                     RFLTVQIFGVILILFGVWGANALSRKRLEGMK"
     gene            1130..3130
                     /locus_tag="PLD15_10120"
     CDS             1130..3130
                     /locus_tag="PLD15_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPB64307.1"
                     /translation="MRAKISLIFFVTIAVGLLQAVQFFVNHGSAPLLNVVEGEVRPME
                     PLENNWWWVDIQLQPGVYNYFFESGGERFTDPGNSRTALVDGKEVSTVTVGDDSVRHI
                     IGDRDYFNPVNEGEIYLAVSTPSDSDYRVIAVSGEKRFELTYSRDFGKIRFYRSRPLA
                     LEEVSYYFEIHIEEEILFLDATGLKNHLERPFEFSRQALPVSFFDTPEWSKGALYYQI
                     FPERFANGNPGNDPENVQQWNTDTGTANLGGDGFFGGDLQGIIDRFDHLLELGVEAVY
                     LNPIFESPSSHKYDTQDYMQIDDNFGDDETFGELSSLAYDYGIRLILDGVFNHTGYDF
                     FAFKDLRENGENSPYKDWYFVKSFPIRKVRDRALSYVGWNGYAYMPKINSLNPQWQDY
                     IKELVEKYDIDGVGGWRLDVATEVAPEFWSDFFRPLVKSLGEETILVAEFWGDAKALL
                     RGKSFDSAMNYPFKDAVVEYVFRAGNSAGRFAAMTNFYLNAYPPQTLHSLWNILDSHD
                     TERIMTLAFNQRDLMKIAVAIQMTFMGSPVIYYGDEVGMKGGKDPENRAPMIWDDQRW
                     DTEIYDFYRKLVEIRKTHPAVKTGDYSVLVSEGSVFGYIRSLGEDRVVVLTNPSRERA
                     TFTMSLEGKYVENLTGREFDFKPGTFEIPPTTVWILTLNGEQ"
     gene            complement(3176..>3885)
                     /locus_tag="PLD15_10125"
     CDS             complement(3176..>3885)
                     /locus_tag="PLD15_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPB64308.1"
                     /translation="PVQAYRIGLKIIREALGSDCFILGCGAPLLPSVGFVDGMRIGAD
                     TAPSWRADVMDIGIPSAKLSIRNAFTRSFMHRRLWLNDPDTIILRDCELTADEKRTFA
                     LSAGLLDGMILTSDDMSLVSREGISLLKEALELTGGKPRVFLDGMNGVFAACTKAARK
                     YNVTAFVNLTDTPRHGIKLKSYYRDWSGVTPSMAEADLFPRKIWLEKVETRARELEKI
                     VEIREDGRQFNYYTEKE"
BASE COUNT          917 a         1026 c         1032 g          910 t
ORIGIN      
        1 cgtgttggta ccagtcaaaa tccctgaaag tatacccgaa agaaatcgcg gtaaaaagca
       61 agaaaacaga caggaaaaac tttccggaca actgcaaagc accaccctct ggaatatctt
      121 aatctgtact actattatag actatgaggg ctctccggag tgcctgaacg gttgttcgaa
      181 caataaaatc cccttgaggt gttccaatgg cggaaaattc tggaagtaaa aacgtagctt
      241 atttcgcggg tatcggctct tccgtgatct ttggtctctc ctttctcttc accaagagcg
      301 cgctcgagta caccaatccc gtgtattttc tctcctacag attcttcata gccttcatca
      361 cgatcaacct tctgagagtt tttggggcga tacgtataag accctaccgg caggttttca
      421 aagaactgtt tttcctgggt ctggtccagc ccgttctgta tttcattctt gaaaacgccg
      481 gtgtctctct gtcaacctcg tcggaagccg ggatacttct atctacgatc ccgatcttcg
      541 tgatgatact ggccaggata ttcctcaaag agaagatcgc gcttccgaga gtcctctcga
      601 tactcacggc tctggccgga atacttctga taactgccgg gaagggtctt tcatcgggcg
      661 gaactgtcct gggaccagtg cttcttctgg ggagcaccct tacggcggcc atgtacaaca
      721 tcctctccag aaaggcttcc gcaaagttca ccccctggga gataacctat cacatgatga
      781 ccgcgggctt ttcgaccttc ttcaccatgg cgcttgtcct gtctcttctc agaggagagc
      841 cgttcgagta tctatctcag ttcttagtgc cgtcggtttt caacgccgcc ctatacctgg
      901 ggacactctc atctgtcggg gcctttttcc tgataaacct catgctttca aagctggagg
      961 cgtcaagggc tacggtcttt gcatacctat ccaccgtggt atctctactt gccggtataa
     1021 ttgtgagggg cgagaggttt ctaacggtac agatttttgg agttatcctg atactattcg
     1081 gcgtatgggg agccaacgcc ctcagcagaa agagattgga ggggatgaaa tgagagcaaa
     1141 gatttctctg attttctttg tgacaattgc cgtcggcctt ctccaagccg tgcagttctt
     1201 cgtgaatcac ggttcggccc ctctcttgaa cgtggtggaa ggagaggtca ggccgatgga
     1261 gccgctggag aacaactggt ggtgggtgga tattcagcta caaccgggag tatacaacta
     1321 cttcttcgag tctggcgggg agaggttcac cgacccggga aactcccgca ccgccttggt
     1381 cgacggaaag gaagtatcga cagtgacggt cggggacgat tccgtcaggc acatcatagg
     1441 cgacagggac tacttcaacc ctgtaaacga aggtgagatt tatctggccg tttcaactcc
     1501 atccgatagt gactaccgtg taatagcggt ctcgggagag aagagattcg agctgacata
     1561 ctccagagac ttcggaaaga tcagattcta ccggtcccgg ccgctcgccc tcgaagaggt
     1621 gagttactac ttcgagatcc acatagaaga ggaaatcctc tttctcgacg cgaccggtct
     1681 gaaaaaccat ctggaaagac ctttcgaatt ttcccgccag gcgctcccgg ttagtttctt
     1741 cgacacgccc gaatggtcga agggcgccct gtattatcag atcttccccg agaggttcgc
     1801 caacggaaac cccggcaacg atcccgaaaa cgtccagcaa tggaacacag atacgggaac
     1861 ggccaacctc ggcggagacg gtttcttcgg cggagacctt cagggtataa tcgatcgctt
     1921 cgaccatctt ctcgagctgg gtgtcgaggc cgtatatctc aaccccatct tcgagagccc
     1981 ttccagtcac aaatacgata cgcaggatta tatgcaaata gacgacaact tcggggatga
     2041 cgaaaccttc ggggaactct catcgctggc ctacgattac gggataaggt tgatactcga
     2101 cggcgtcttc aaccataccg gctacgattt cttcgccttc aaagatctca gggagaatgg
     2161 cgagaactct ccctacaaag actggtactt cgtcaagagc ttccctatac gcaaggtaag
     2221 ggacagagcc ctgtcgtacg tgggatggaa cggctacgcc tacatgccga aaatcaactc
     2281 tctcaatcct cagtggcagg actatataaa ggaactcgtc gagaagtacg acatcgacgg
     2341 agtaggcgga tggcgactgg atgtcgcgac cgaagtggct ccggagttct ggagcgactt
     2401 cttcagacct ctggtcaaga gtctcgggga agagacgata ctggtcgccg aattctgggg
     2461 agatgcgaag gccctactgc ggggaaagag cttcgattcg gccatgaact atcccttcaa
     2521 ggacgccgtt gtggagtacg ttttcagggc gggcaactcg gccggcaggt tcgcggccat
     2581 gaccaacttc tatctgaacg cttacccgcc gcagacgctc cactccctct ggaatatact
     2641 agacagtcac gatacggaaa ggataatgac actggccttc aaccagagag atctcatgaa
     2701 gatcgccgtg gccatccaga tgactttcat ggggagcccg gtgatttatt acggcgatga
     2761 ggtaggtatg aagggcggaa aggatcccga gaacagggct cccatgatct gggacgacca
     2821 aaggtgggac acggagatct acgatttcta cagaaagctt gtagagatca ggaaaaccca
     2881 tccggccgtg aaaaccggag attactcggt gctggtgagc gaggggtctg tgttcggtta
     2941 catcagatct ctaggtgagg acagagtggt tgtcctgacc aacccttcga gagagagggc
     3001 gactttcacc atgtctctcg aggggaaata tgtagaaaac ctcaccggcc gggagttcga
     3061 tttcaagccc ggaactttcg agataccgcc cacgacggtc tggatactga cgctgaacgg
     3121 cgaacaataa tcggaagcat ccgtcgtcaa acgagagtga gagaagcgca ggctctcact
     3181 ctttttccgt gtagtagttg aactgtctgc cgtcctccct gatctcgacg attttctcga
     3241 gttcccgggc cctggtttca accttctcca gccatatctt tctggggaaa agatcggcct
     3301 cggccatcga aggggttaca ccggaccagt cccggtaata gctcttgagc tttatcccgt
     3361 gtctgggcgt atcggtaagg ttgacgaagg ccgttacgtt gtactttctg gctgccttgg
     3421 tgcaggccgc aaagactccg ttcataccgt cgagaaagac cctgggcttg ccccccgtaa
     3481 gttcgagcgc ttccttcagc agagaaattc cctcccggct caccagggac atatcgtcgc
     3541 tggttagaat cataccgtca agcaggccgg ccgaaagcgc gaacgtcctt ttctcgtcgg
     3601 ctgtcagttc gcaatcgcgg agtattatgg tatcgggatc gttcagccag agtcttctgt
     3661 gcatgaagga acgggtgaag gcgttcctga tcgaaagttt cgcgctcggg atacctatat
     3721 ccatcacgtc ggccctccag gatggcgcgg tatcggcgcc gatgcgcata ccatcgacga
     3781 agccgaccga tggaagcagg ggtgcgccgc aaccgagtat gaaacagtcg cttccgagcg
     3841 cctcccttat tatcttcagg ccgattcgat aggcctgtac aggtg
//
LOCUS       DAOOLY010000234         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_28919_length_3822_cov_5.990974, whole genome shotgun sequence.
ACCESSION   DAOOLY010000234 DAOOLY010000000
VERSION     DAOOLY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28919_length_3822_cov_5.990974"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2971
                     /locus_tag="PLD15_10130"
     CDS             <1..2971
                     /locus_tag="PLD15_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPB64309.1"
                     /translation="VRQELLLRGYSLLSDRFVPVWSLARRLASSIAGELRQIPEGVCR
                     MELSAVIQELKGYVPSARTVGYFREAVNVLKENMGDLGLAFGGEEAVPQILLQVYEGL
                     KKRFQERRYFDMYDAFAIVAENADKIESGSYGTVLFIDGFNDLSPAMAAFVAKFMEGF
                     ESVYLTVPSDPDRRGLFREAGTLPGLFEGRECENIELEDGYDGPLAAVKNSLFRDAAL
                     PAGEEVEGVGVVECNDLYGEVDYVSRQIKRLIVLEGERPESFEIVVPGDDYLSVLKKR
                     LADFDIPCVSRWPGSLLQSKSVRHLLLPFEVVSASFEPQKVLEMVDAGYGNPGPGRQV
                     NAVMFEKIASAAGLLYGATQESAAEAGKFWTKNLESYKGFLQGKRKRLEEQNDEGDDF
                     SLSSLISETADQAVFVAGEMLPAVRKIFTFLGPLDTDRSLSLRKLSGLFSAWWRQLGL
                     DSLSESPEISATAGESLELCLRFLQKALPDHDRQLRLAGIEECPPAFYHRSLLAFLNS
                     DGLVSERNMGGGVSVVSLVDSRNIEAKYKFFMGFNDGNYPVTGLNPLYSRAQYSRPVA
                     KDILNIQENRQRLGLFLAVAAAQERAIFTFSNSTIDGDSLLASPYTQSLLDAAGKAGT
                     LVHGRDGGKRSDLIGSPAGAMSRGDFRLAVARIFALDRETWDANATSDQTRRLEENLR
                     RVPREFDYNVSMKAAREEQETRFVSFSKISQYCGCKFKYFLSNVLKIEEELEEILELS
                     HMDTGSVFHAVLSQLPRSDGEIETVLDGQLRRYSSSENESLFRLRKKQMTGILSEYIA
                     FDSERTRGLNMRTEKTELAFGFGTVSPLELPGLCMRGFVDRVDVKNDDGTLFVIDYKS
                     GKNTSSAHPEQLVLYSMAVEQAYRYEKRVGAGEFRLIKGKNKTKPFIVEWDQDGRKWV
                     FDGRVGFTICETEKKGTITDGELVEAIRKIVAAIDAGDFSMTDWKGKKPNCFNCAYDK
                     LCGMLEWRNRGVLANDR"
     gene            2961..>3822
                     /locus_tag="PLD15_10135"
     CDS             2961..>3822
                     /locus_tag="PLD15_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB64310.1"
                     /translation="MIGDTFISASAGTGKTYRLVKEYTDILGGDPSLDLDNVLAITFT
                     EKAAKEMKERVSRKIAEKLQEAATDDERARWKRLGDRIMDAWISTIHSFCDRLLRESL
                     LYTDYGIDPGFSIITGTKKLIENRTLRRYCIKNLGSLESLTDALGLDATYRLLEEALG
                     KERHNLYIYKPFQADRQDDRAAQESARLTTGFFEAFGRINAQYEEATLKNGQLDFEQL
                     LELTRRILNDYPEIARDYARRFRYILVDEFQDTDELQAEIIGKIRESGDVKVLYVGDD
                     KQSIYRFRGAN"
BASE COUNT          851 a         1074 c         1219 g          678 t
ORIGIN      
        1 cgtccgccag gagttgctac tgagaggcta ttcgctcctc tccgacaggt tcgtgccggt
       61 gtggtcgctc gctaggcggc tggcctcgtc tattgcaggc gagctgaggc agatcccgga
      121 gggcgtttgc aggatggagc tgtcggcggt cattcaggag ttgaaaggat acgtgccctc
      181 ggcccgcacc gttggctact tccgcgaggc ggtcaacgta ctgaaggaga acatgggcga
      241 tcttggcctc gccttcggcg gtgaggaggc cgtcccgcag atcctcctgc aagtatatga
      301 aggcctgaaa aaacggtttc aagagcggcg ttacttcgat atgtacgacg ccttcgcgat
      361 cgtcgccgaa aacgccgaca aaatagagtc gggcagctac ggaaccgtcc tgttcataga
      421 cggtttcaac gacctgtcgc ctgcgatggc ggccttcgtg gcgaaattca tggagggctt
      481 cgagagtgtg tatctcactg ttccgtccga ccccgacagg cgcgggctct tccgggaagc
      541 cggtacgctc cccgggcttt tcgagggcag agagtgcgag aacatagaac tggaagacgg
      601 gtatgacggt ccgctcgccg ccgtgaagaa ctcgctcttc cgcgacgcgg cgctgcccgc
      661 cggcgaggaa gtcgaagggg tcggggtcgt cgagtgcaac gatctgtacg gcgaggtcga
      721 ttacgtttca aggcagataa agcggcttat cgtcctggag ggcgaaaggc cggagagttt
      781 cgagatagtc gtgccggggg acgactacct gagtgtgctg aagaagagac tcgcggactt
      841 cgacatcccc tgtgtcagcc gctggcccgg ctcgctcctg cagtccaaga gcgtccggca
      901 cctgctcctc cccttcgagg tcgtctccgc ctccttcgag ccgcagaagg ttctcgagat
      961 ggtcgatgcc ggttacggaa accccgggcc cggcaggcag gtcaacgccg tcatgttcga
     1021 gaagatagcc tccgcggccg gcctgttgta cggcgccacc caggagagcg cggccgaagc
     1081 gggaaaattc tggacgaaaa acctcgagtc gtacaaaggt tttctccagg gcaagcgcaa
     1141 gaggttagag gagcagaacg acgagggcga cgacttctcg ctgagctctc ttatctcgga
     1201 gacagccgat caggccgtct ttgtcgccgg agagatgctg cccgcggtga ggaagatctt
     1261 cacctttctc ggaccgctcg acacggacag aagcctgtcg ctccggaagc tttccgggct
     1321 gttttcggcc tggtggaggc agctgggtct ggatagtcta tcggaaagcc cggaaatctc
     1381 cgctacggcc ggggagtctc tggagctgtg cctccgcttc ctgcagaagg cgctgcccga
     1441 ccacgacagg caactgaggc tggcagggat cgaagagtgc ccgccggcct tctaccaccg
     1501 ttcgctcctg gccttcctga acagcgacgg cctggtcagc gagagaaaca tggggggcgg
     1561 cgtgagcgtc gtctccctgg tcgattcgag gaacatcgaa gccaaataca agttcttcat
     1621 gggcttcaac gacgggaatt atcccgtcac cggccttaac ccgctctatt ccagggcgca
     1681 gtactcgagg cccgtcgcca aagacatact gaacatacag gagaaccgcc agaggctcgg
     1741 cctcttcctc gcggtggccg ccgcgcagga gagggctatc ttcacgtttt ccaattcgac
     1801 gatcgacgga gactcgctgc tggcctcacc ctacacgcag tctttgctgg acgccgccgg
     1861 caaggcggga acgctggtcc acggcaggga cggcgggaag aggtccgatc ttattggctc
     1921 gcccgccggt gcgatgagcc ggggagattt caggctggcc gtcgcgcgga tattcgcgct
     1981 ggaccgcgaa acgtgggacg ccaacgcgac ttccgatcag acgaggcggc tcgaagagaa
     2041 tctcaggaga gtgccccgcg aattcgatta caatgtgtcg atgaaggccg ctagggagga
     2101 gcaagagacg cgtttcgtga gcttttcgaa gatcagccag tactgcggct gcaaattcaa
     2161 gtatttcctg agcaacgtac tgaagatcga ggaagagctg gaagagatcc tggagctctc
     2221 ccacatggac acgggcagcg tctttcacgc cgtgctcagc cagctgccgc ggagcgacgg
     2281 agagatagaa acggtcctcg acggacagct gcgcaggtac tccagcagcg aaaacgagag
     2341 cctcttcagg ctccgcaaaa agcagatgac cggcatcttg agcgagtaca tcgccttcga
     2401 cagcgagagg acgcgcgggc tgaacatgag gacagagaaa acggagctcg ccttcggctt
     2461 cggcactgtg tcgccgctcg aactgcccgg actctgcatg aggggcttcg ttgacagggt
     2521 agacgtgaaa aacgacgacg gtacgctctt cgtaattgac tacaagagcg gaaagaacac
     2581 gtcttcggcc catccggagc agctcgtcct gtattccatg gccgtcgagc aggcgtacag
     2641 gtacgagaag agagtcggcg ccggagagtt caggctgatc aagggtaaga ataaaactaa
     2701 gcctttcata gtggagtggg atcaggacgg caggaaatgg gtcttcgacg gcagggtcgg
     2761 cttcacgatc tgcgagacag aaaaaaaggg cacgatcacg gacggagagc tcgtggaggc
     2821 gatccggaaa atcgttgccg ccatcgacgc cggcgacttc tctatgaccg actggaaggg
     2881 aaagaagccc aactgcttca actgcgccta cgataagctc tgcgggatgc tcgaatggag
     2941 aaacaggggg gtgctcgcga atgatcggtg acacgttcat ctcggcctct gcgggcacgg
     3001 ggaagactta caggctggtc aaggagtaca ccgacatact cggcggcgat ccgtcgctcg
     3061 atctcgacaa cgtcctggcg ataaccttca ccgagaaggc cgcgaaggag atgaaggaga
     3121 gggtgtccag gaagatcgcg gaaaagttgc aggaggcggc caccgacgac gagcgggcca
     3181 ggtggaagag actgggcgac aggataatgg acgcctggat atctacgata cactctttct
     3241 gcgacaggct gctgagggaa tcgctcctgt acaccgacta cgggatcgac ccgggcttct
     3301 ccataatcac cgggacgaag aaactcatag agaacaggac gctcagacgg tactgtatta
     3361 aaaacctggg cagtctcgaa tcgctgaccg acgccctcgg cctcgacgcc acctacaggc
     3421 tgctggaaga ggctctcggc aaggagaggc acaacctgta catatacaaa cccttccagg
     3481 ccgaccggca ggacgaccgg gcggcgcagg agtcggcccg cctaaccacc ggctttttcg
     3541 aggccttcgg ccggatcaac gcgcagtacg aggaggcgac cctgaagaac ggccagctcg
     3601 acttcgagca gctactcgag ctcaccagaa ggattctgaa cgactacccg gagatagcaa
     3661 gagactatgc ccgcaggttc aggtacatcc tcgtggacga gttccaggac accgacgagc
     3721 tgcaggccga gattattggg aagataagag agtccggaga cgtcaaggtc ctgtacgtcg
     3781 gcgacgacaa gcagtctata taccgtttca ggggagcgaa cg
//
LOCUS       DAOOLY010000235         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29136_length_3800_cov_5.356742, whole genome shotgun sequence.
ACCESSION   DAOOLY010000235 DAOOLY010000000
VERSION     DAOOLY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29136_length_3800_cov_5.356742"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1463
                     /locus_tag="PLD15_10140"
     CDS             18..1463
                     /locus_tag="PLD15_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPB64311.1"
                     /translation="MQFPFERLFNIKDLTNFIIYPIFLALLNRAPFLSFPSKPFLYFI
                     GSAILWYIFAERTVKNNRYFHLHRAYRLTFFCVVAFGVFINSFLPSLADAKLLPEMTP
                     IFLSVSMVTILIGYQEGIGSGLFMSFLASANIDNSFETFILLSVVVLSTALTTRHIER
                     RIEIARAGFEVSLIFTAVNLVRMTLQGEPISALDLTVAFANPILSSVIVIGIVPYIEY
                     VSRIYSDTGMLELGNLSHPLLKNLSIYAPGTYYHSISLANLSEAAAEKIGANPILARV
                     AAYFHDAGKSKRPQYFVENQQDGINPHDNITPSMSNLVINEHVKQGVELAHKYRLPLL
                     IEDVVKEHHGTRIKKFFFHKAKTGGQEEHQEDFQYPGPKPRFKESGIIMLADSVEAAY
                     KSLKKPTPAKTQELIEEVVNGIYNERQLDQTGLDLDDLEKIIEAFTRVLMSMNQPRIE
                     YPKETIEKVVMANGDNSPKQDGKDDRREKSE"
     gene            1405..1830
                     /gene="ybeY"
                     /locus_tag="PLD15_10145"
     CDS             1405..1830
                     /gene="ybeY"
                     /locus_tag="PLD15_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPB64312.1"
                     /translation="MEIIVQNKMERTIDAKKVSNIARKVIEKELGETEIGSLNILLTD
                     DAEIASINKQFRNKEEPTDILSFGYGLEEEPIGDIVISLERIAEQSQEFGNSFEEELL
                     YITIHGVLHVLGYDHEGYDTEDEEIFLLQKKYFRELVKE"
     gene            1837..3078
                     /locus_tag="PLD15_10150"
     CDS             1837..3078
                     /locus_tag="PLD15_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPB64313.1"
                     /translation="MSDFRPFRAVRPAAGLEQKVNCPPYDVISFEEAREIGRNPESFV
                     HVIRSEIDLPEETDHYDESVYLKAKGNFEKLLSDAILVQEERPAYYIYWQKMKDHVQI
                     GIVGCASVDEYQRERIKKHELTRQDKEEDRVKHIHTVGANTGLVFLTFRSSEKLEEIL
                     SRYVKELELSMRVIDENDVEHRLYAVKDDKRVEELRQSLGEIENMYIADGHHRAASSS
                     RVRELMKAQNPDHTGKEEYNYFMAVAFPHSYLRIMDYNRVVKDLGGISPEEFMDRVGA
                     AFDISHAPESPYKPVKRHEFGMCLYGKWYVLTARSGTFDEDDPVGSLDVDILQRNLLG
                     PVLGIDNPRKDPRIDFVGGIRGLKALENRITNGWAVAFSMFPTSMEELLAVADSGKIM
                     PPKSTWFEPKLRSGLVVHLLK"
     gene            3075..3797
                     /gene="ispD"
                     /locus_tag="PLD15_10155"
     CDS             3075..3797
                     /gene="ispD"
                     /locus_tag="PLD15_10155"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPB64314.1"
                     /translation="MKTVAIVVAGGRGDRMKASIPKQFMLLQHREIFVRSVEIFHGSD
                     LIDGVVLVMHPDWMEAAGRILDKYGIDSVGIVPGGETRQESVYRGLCSIEYEGFDTVL
                     IHDAARPLFSAFQIPDLLKTVEKGLGVVVVEPLSDTVYITDHGSVVGIPDRANLRAAE
                     TPQTFRYEDIFAAHRKAIDENLTSSTDDARLLLNNGCKIVTLDSLTRNMKITGPQDLE
                     IAELLLKSRAKTKGESFDSPIE"
BASE COUNT         1107 a          890 c          964 g          839 t
ORIGIN      
        1 ataaagagaa aaagaaaatc caatttccct ttgagagact cttcaacata aaggatctca
       61 ccaatttcat aatctatccg atttttcttg ctcttttaaa ccgggctccc tttctttcct
      121 tcccgtcgaa gccctttctc tatttcatcg gttctgcgat tttgtggtat atattcgccg
      181 aaagaaccgt taaaaataac agatacttcc atctacacag ggcatacaga ttgacatttt
      241 tctgtgtggt tgctttcggt gtgttcataa actcttttct gccttcgctg gccgatgcga
      301 agctcctgcc ggagatgacg cccatcttcc tgagcgtctc gatggtgacc atattgatag
      361 gttatcagga aggaatagga agcggtctct tcatgtcatt tctggcctcc gccaatatcg
      421 acaactcgtt tgagactttc atactcctgt ctgtcgtcgt tctctcaacg gcactgacaa
      481 caaggcatat cgaaaggaga atagagattg ccagggccgg cttcgaggtc agtctgatct
      541 tcacggccgt gaatctcgta agaatgactc tccagggaga accgatcagc gctctggatc
      601 ttacagttgc cttcgcaaac ccaatcctct cttcggtgat agttataggc atagtgcctt
      661 acatagaata tgtgagcaga atctattcgg atacggggat gctggagctg gggaatttgt
      721 ctcatccgct gttgaaaaat ctgtcgatat atgcgccggg tacgtactac cacagtattt
      781 ctctggcaaa tctatccgag gcggccgccg aaaagatcgg agccaaccct atacttgcca
      841 gagtggctgc gtacttccac gacgccggaa aatccaagag accccagtac ttcgtagaga
      901 accagcagga tgggataaac ccgcacgaca atatcacacc ttcgatgtcg aacctggtca
      961 tcaacgaaca tgtgaagcaa ggcgtcgagc tggcccataa ataccgtctt cctctgttga
     1021 tagaagatgt cgtgaaggaa caccacggaa ccagaataaa gaagttcttc ttccacaagg
     1081 ccaaaaccgg tggtcaggaa gaacaccagg aagacttcca gtaccccgga ccgaaacccc
     1141 ggttcaagga atcgggcatc ataatgcttg ccgactcggt tgaagctgcc tacaagagcc
     1201 tgaaaaagcc tacacccgca aagacccagg aactgataga agaagtggtc aacggcattt
     1261 acaacgaaag acagctcgat cagacggggc tggacctcga cgatctcgag aagataatcg
     1321 aagccttcac gagggtcctg atgagcatga atcagccaag aattgaatat ccgaaagaga
     1381 cgatagaaaa agtggtgatg gcaaatggag ataatagtcc aaaacaagat ggaaaggacg
     1441 atagacgcga aaaaagtgag taacatagcc aggaaagtaa ttgagaaaga actcggcgaa
     1501 accgaaatcg gaagtctgaa catccttctt acagatgacg cagaaatagc gagtataaac
     1561 aagcaattca gaaacaaaga agaaccaacg gacattcttt ccttcggata cggtttggaa
     1621 gaagagccga taggtgatat agtcatttcg ctggagagaa tagccgaaca atctcaagaa
     1681 ttcggaaact ccttcgaaga ggaactcctc tacattacga tccatggagt ccttcacgtc
     1741 cttggctacg accatgaggg gtacgacacc gaagacgaag agatctttct tcttcagaaa
     1801 aaatactttc gagaactggt aaaggagtga ttggctttgt ctgatttcag acctttcagg
     1861 gctgttagac cggctgcggg attggagcag aaggtcaact gtccgccgta cgatgtcatt
     1921 tcgttcgaag aggcaagaga gataggtaga aacccggaaa gtttcgtgca cgttattaga
     1981 tcggagatcg atcttcccga agagaccgac cattacgacg agagcgttta tttgaaagcg
     2041 aagggaaatt tcgaaaaact tctttccgat gctattctgg tacaggaaga gagacctgcc
     2101 tactatatat actggcagaa gatgaaagat catgtccaga taggaatcgt aggctgcgcc
     2161 agcgtcgatg agtatcaaag ggagagaata aaaaagcacg agctcaccag gcaggacaaa
     2221 gaagaggaca gggtaaaaca tatccacacc gttggagcca acaccggact ggtatttttg
     2281 accttcaggt ccagtgaaaa actcgaagag atcctttcta gatacgtgaa agagctggaa
     2341 ctctctatga gagtcatcga cgaaaacgat gtggaacaca ggctctacgc agtcaaagac
     2401 gataagagag tcgaggagtt gaggcaatcg ctcggggaga tagaaaacat gtacatcgcc
     2461 gacggtcacc acagagccgc ttcctcttcg agggtcaggg agctgatgaa agctcaaaat
     2521 ccagaccaca caggcaagga agaatacaat tacttcatgg ccgtcgcctt tccacactct
     2581 tacctcagga taatggacta caacagggtg gtcaaagacc tcgggggaat ctccccggaa
     2641 gaattcatgg atagggtggg ggccgctttc gacatatccc atgccccaga gagcccatac
     2701 aaacctgtaa agagacatga attcggtatg tgcctgtacg gaaagtggta cgttctgacc
     2761 gccaggagcg gaactttcga tgaggacgac ccggttggaa gtctggacgt ggatatcctt
     2821 cagagaaacc tcctgggacc tgtactggga atcgacaatc ccagaaagga cccgagaata
     2881 gacttcgtag gaggtataag aggtttgaag gctctggaga acagaataac caacggctgg
     2941 gccgtcgctt tttcgatgtt cccgacctcg atggaggaat tgctggctgt cgccgacagc
     3001 gggaagataa tgcctccgaa atccacctgg ttcgaaccga aactgcgcag tggattggtc
     3061 gtgcatcttc tgaaatgaaa accgtggcga ttgtcgttgc aggcggccgg ggagacagga
     3121 tgaaggcatc catccccaag cagttcatgt tgcttcaaca tagagagata ttcgtcagat
     3181 ccgtggagat atttcacgga tctgatttaa tagatggagt cgttctcgtc atgcatccag
     3241 actggatgga agctgccggg agaatacttg acaaatacgg tatagattcg gtgggcatcg
     3301 tccccggggg agagacgagg caggaatccg tttacagggg actttgttcg atcgaatacg
     3361 aggggttcga caccgttttg attcacgacg cggcaagacc gcttttctcg gccttccaga
     3421 tcccagattt gctcaaaacg gtcgaaaagg gccttggcgt ggtagtcgta gaaccgttga
     3481 gcgatacggt atacatcacc gaccatggat cggtggtggg gatccccgac cgtgcaaacc
     3541 tcagggccgc cgaaacgccg cagaccttca gatacgaaga tatctttgcg gctcaccgca
     3601 aagccataga tgaaaacctg acctcttcca cggacgatgc gaggctgctg ctgaacaacg
     3661 gatgcaagat cgtcacgctg gactctctca ccagaaatat gaaaatcacc ggcccccagg
     3721 atctcgagat agcagaactg ttgctgaagt cacgcgcaaa aacaaaagga gagtcttttg
     3781 actctccaat tgaataacat
//
LOCUS       DAOOLY010000236         3792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29211_length_3792_cov_4.606904, whole genome shotgun sequence.
ACCESSION   DAOOLY010000236 DAOOLY010000000
VERSION     DAOOLY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29211_length_3792_cov_4.606904"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1285
                     /locus_tag="PLD15_10160"
     CDS             398..1285
                     /locus_tag="PLD15_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HPB64315.1"
                     /translation="MASAFDVSCRTLRYYEEIGLLKSFRNEESLYRYYDEASLKKLEE
                     ILFLKSLGLSMKEILKVLTIYDMEKFKTSLVNRLETLLDESNTISRERSRIEKLLVHL
                     DSGVGVKLSDIMAELYHSEEDRQQQVRSYEEEEKVRSPRECLDVRIVQLKPCRVAYYR
                     SSGKSPEYDVLRVMEEWASRRGLLDLFSTRNFGFNNPSPSPESEEYGYEVWLTVTQET
                     EPSGGVGMKDFSGGLYAVISTHLHDIAENWKAISRWVESNDEYCAGRHQCLEEIISPD
                     WFDESAVQFDLYYPILRRQ"
     gene            1683..2129
                     /locus_tag="PLD15_10165"
     CDS             1683..2129
                     /locus_tag="PLD15_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPB64316.1"
                     /translation="MEEQLIAPCGMNCSLCISYQSMKNDLKRQGFHKKYCPGCIPRGE
                     NCIHMGDQCELLAKGKIRFCYECEIFPCKRLKTLDKRYRTKYHMSMIENLEFIRDNSV
                     ELFLKEEEIKWLCNECGGVICCHNGLCLNCDLETLRKNKKYRWKEE"
     gene            2224..2634
                     /locus_tag="PLD15_10170"
     CDS             2224..2634
                     /locus_tag="PLD15_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64317.1"
                     /translation="MSNLRTYLEEKNPDYTTGALYYGYMDMTYFAFTPSNLKSRKLKI
                     AVVYLHEPNSFEVWLGGNNRKIQAEYIELFSNKGTGGYKLSRVSPGVDSIIESTLSEQ
                     PDFDNPGELMRQIESKVIECVERIVFILGEQLER"
     gene            complement(2686..3753)
                     /gene="corA"
                     /locus_tag="PLD15_10175"
     CDS             complement(2686..3753)
                     /gene="corA"
                     /locus_tag="PLD15_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPB64318.1"
                     /translation="MAKSRLFTKRKPPSGSVPGTLSIDESSPFPIMKLITFNEKEIEE
                     VTVEDPSLLEEHIFEKGRVNWIDVQGLGDEKTVRKLGEIFKLHPLALEDITNVPQIPK
                     IEEYENGLFICLRMIRLEEKEVISEQVSFFVGETFVLTFQERYGDVFDPVRQRMRRGS
                     TIRKLGNDFTVYALIDTIIDNYFPVLQDTGSSLEALEDEMILKINKEKLRKLYELKHG
                     LAELKRILWPTRDAIGQLARNENSYIDGKTALYFRDVYDHVIQSIDMLDLYRELASEL
                     MNVYLSSMSNRMNEIMKVLTIISTIFIPLSFVAGVYGMNFRFMPELELKWAYPIVLGA
                     MVAIAGAMLYFFHKKGWFRKD"
BASE COUNT         1025 a          805 c          955 g         1007 t
ORIGIN      
        1 cgaagtggga cagagagaag accgtttcct ggaccgtaac cggtactgga accgcgacga
       61 tcaccttctc ttctaccaga ggcggagtca tctctacgac cgttgagctg aagtaggaat
      121 tagcaagtct gatctctgaa acaggcaaca agtatttata gtgattgttg tttcttgatc
      181 cacataaaaa gaaagatttg gtctacctcc ttgggtgtcc tccagaggtc tggaggacac
      241 cttttttatg aagtaagtgg cagacacaat ggtttttctc aatgaattat aaccgttgat
      301 ggtattgact ctcccgttac ggcaaagagt ataatctcct ctgtcagatt aaattcgttc
      361 tatcggggtg gtgatctgaa tcaagaagat cggcgaggtg gcttctgctt tcgatgtatc
      421 ctgtcgtact ctgagatact acgaagagat cggcttgctc aagagttttc gaaatgagga
      481 gtctctgtac cgttactacg atgaagcctc tctgaagaag ctcgaggaaa ttctctttct
      541 gaaaagtctt ggtttatcta tgaaggagat tctaaaagtc cttacgattt acgatatgga
      601 aaaattcaaa acctcactgg tcaacaggct ggaaactttg ctggatgaaa gtaatactat
      661 cagtcgtgag agatccagaa tagaaaaact tcttgtacat ctcgacagcg gtgtcggcgt
      721 aaagctttcg gatataatgg ccgaacttta tcatagtgaa gaagataggc aacaacaggt
      781 aagatcttac gaggaggaag aaaaagtgag aagcccgaga gaatgccttg atgtaaggat
      841 tgttcaattg aagccgtgca gggtcgcata ttacaggtcc agtgggaaga gtcccgagta
      901 cgatgtgctg agagtaatgg aggagtgggc ttctagaaga ggtttgctag accttttctc
      961 aacaagaaac ttcgggttca acaatccttc gccttctcct gaaagcgaag agtacggtta
     1021 cgaagtctgg ttgacagtta ctcaggaaac ggagccttct ggcggggtgg gaatgaagga
     1081 tttctccggc ggactctatg ctgtaatcag cacccacctt cacgacatag cggagaactg
     1141 gaaggcgata tcccggtggg tcgaaagtaa cgatgagtat tgtgccggaa gacatcagtg
     1201 tctggaagag ataatttccc cggactggtt cgatgaatct gctgtccagt ttgatctcta
     1261 ttatccgatt ctcaggaggc aatagatcgt ctgaaattga tactagaaaa ccgggccagg
     1321 gcagagtggg ttgtgcgttg tggattgtcg gtaaaaacac gagaaagagc gtttagagct
     1381 ccctttgctc atgagaaggc gagagcgaaa atagggactg acgggctcct ggcgtctgtc
     1441 ccgtttttcg cgacgtgaag ggagtgagac tgtcatcccg tagtgtcggg atctaatctc
     1501 ttccgtctcg cactctctgg agcgttcgtc gtgagaggtg tgaggtgaga aggttctcag
     1561 atcttgatct tcatacctct tacctctccg tcccctcgtc gcccgcatct attaaatggt
     1621 atgatctatc aaaagatttg tacagggccg gctcaataga tgatggctaa aagaggtggc
     1681 gtatggaaga gcagttaatc gctccatgcg gtatgaattg cagtctgtgc atcagttatc
     1741 agtcgatgaa aaacgacttg aaaaggcagg gatttcacaa aaagtactgt cccggctgta
     1801 tcccgagggg ggagaattgc atccacatgg gcgatcagtg cgagctgctt gcgaagggaa
     1861 agatccgctt ctgctatgaa tgtgaaattt ttccctgtaa acgattgaag acgctggata
     1921 agcgctatcg cacgaaatat catatgagca tgatagagaa ccttgagttc atccgcgaca
     1981 actccgtaga gctgtttttg aaagaggaag agataaaatg gctctgcaac gagtgtggcg
     2041 gcgttatatg ctgtcataac ggactttgct tgaattgtga ccttgaaact ctgcgtaaga
     2101 acaaaaaata ccgctggaaa gaggagtagg gctgtggaat cgctgaacga tcttgtacgg
     2161 gaatacaata accagttaag caaaggaagg attcagaaag catatagaga aataatgtcc
     2221 ttcatgtcca atctgagaac atacctggag gaaaagaatc cggactatac aaccggtgct
     2281 ctatactacg gttatatgga catgacatac ttcgcattta caccctccaa tttgaagagc
     2341 aggaagttga aaatcgccgt tgtgtatttg catgagccga acagtttcga agtctggctg
     2401 ggtgggaaca acagaaagat ccaggctgaa tacattgaat tgttcagcaa taaaggcaca
     2461 ggggggtaca aactgtcgcg ggtctctccg ggagtggatt ccataattga atccaccctt
     2521 tctgaacaac cagatttcga taatcccgga gaactgatga gacagatcga aagtaaggta
     2581 atcgaatgcg tagagaggat cgttttcata ctcggtgaac aattagaaag atgaaacact
     2641 gcacaattag gaatgatcgg cacttacata tatctttata tcgcttcaat ctttcctgaa
     2701 ccagcctttt ttatggaaga agtacagcat ggctccggcg atggcgacca tcgctcccag
     2761 tacgatcgga taggcccatt tcagctcaag ttccggcatg aagcggaagt tcattccata
     2821 aacaccggcc acgaaggaaa gcggtatgaa gatcgtggaa attatggtga ggactttcat
     2881 gatttcgttc attctgttgc tcatggaaga aagataaacg ttcatgagct cggaggccag
     2941 ttctctgtaa agatcgagca tatctatgga ctgtatgaca tgatcgtaca catctcgaaa
     3001 atacagtgcc gtctttccgt ctatgtaact attttcgttt cttgccagct ggcctatcgc
     3061 atctctggtc ggccagagaa tccttttcag ttcggcgagg ccgtgcttga gttcgtaaag
     3121 tttccggagc ttttctttgt tgattttgag gatcatctca tcttcgagtg cctccagaga
     3181 gctccctgtg tcctggagta cggggaagta attgtctatg atggtatcta tgagagcata
     3241 aaccgtgaag tcattgccaa gtttccttat ggtggatcca cggcgcatcc tctgtcttac
     3301 gggatcgaaa acatccccgt acctttcctg gaaagtcaaa acgaaagtct caccaacgaa
     3361 aaagctgacc tgttcggaga tcacttcctt ctcttcgagt cttatcatgc gaagacagat
     3421 gaaaaggccg ttttcgtact cctctatttt cgggatctgt gggacgttgg tgatatcctc
     3481 cagagccagt ggatggagct tgaagatctc tcccagcttt ctcaccgttt tttcgtcgcc
     3541 cagaccctga acgtctatcc agtttacccg tcccttctcg aagatgtgct cctctagcaa
     3601 tgagggatct tcgacggtga cctcttctat ctccttctca ttgaaagtta tcagcttcat
     3661 tatcggaaaa ggcgaggact cgtctatgct cagggtgccc ggtacggatc ccgacggcgg
     3721 tttcctcttc gtgaatagcc tgcttttagc cataacttct cgacctccag aaagatatta
     3781 tcacggtcga tg
//
LOCUS       DAOOLY010000237         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29250_length_3789_cov_4.826727, whole genome shotgun sequence.
ACCESSION   DAOOLY010000237 DAOOLY010000000
VERSION     DAOOLY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29250_length_3789_cov_4.826727"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLD15_10180"
     CDS             complement(<1..391)
                     /locus_tag="PLD15_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB64319.1"
                     /translation="MLVPLARPDITEREIEAVAGLMDSGILSIGPKVVEFERIIADYV
                     GLKYAIAVNSGTSALHLIVRSVGLDRGKVMLTSPFTFVSSANVAIYEGAIPVFADIDE
                     KTYNISPETLDEALEEYSRKGLETEKLS"
     gene            complement(392..931)
                     /locus_tag="PLD15_10185"
     CDS             complement(392..931)
                     /locus_tag="PLD15_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64320.1"
                     /translation="MNWYLNFVRGYPFISAMIQFAVLGTLGDLVSKKLSGSKESVSMS
                     LFLSKMLVWAVLSIFIKVAFIGFEGFVNTLVLNRVLPEVFFEKAFLNALARSVAMNLQ
                     FGVFLVLFHRLLDNIVERKRNWKKLDSAFLSLLWFWIPAHTVTFMLPKDYQIGLAALW
                     SFMLGLLLSTFARSTREVE"
     gene            complement(928..2799)
                     /locus_tag="PLD15_10190"
     CDS             complement(928..2799)
                     /locus_tag="PLD15_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPB64321.1"
                     /translation="MFKLKALTGMRRNVALLLLDFLLIYLAYVMGMYFRYGIFNLYEP
                     EFFVNGIFFSLFIVISLALNGTYKIAWSYSYFRDYWIVIRGIATGFVAGFGVGRLLIL
                     LNIKLLTVPFTVSSMAAIGSAFLIIWSRIMWLSFINRGHHALAASERVLIVGAGDAGT
                     SLAEELARHPEHGVVVGFVDDSPRKLRRRIRGIQVLGDSTRIMELVEGFKIGKVIVAI
                     PSADSQTLRKIFSYIDTKKIKVQTLPSLTEIIDGKARLGYLREIDIEDLLGRESVKVD
                     LASLKDYIVERVVLVTGAGGSIGSELCRQIAPLGPGKLLLLGKGENSIYEIKQEISKQ
                     YPDIPVVQLIGDVQDTDRMEYIFKTHRPSLIFHAAAHKHVPLMEENPTEAFRVNSLGS
                     LNVASLAHRYSAEAMVMISTDKAVNPSSIMGASKRLAEEFVRSIAENSATRFGIVRFG
                     NVLGSRGSVIPLFKKQIQSGGPVTVTDPRMTRYFMTIPEAVSLVLQAGAYAAGGDIFV
                     LDMGEPVKISTLAREMITLAGYVPQQEIMIEYTGARPGEKLFEELVMTDEALSKTKHP
                     KIFKLNGQTVLGEKEMEELVSKLELAIRNSDYAVLNGIIQGFMPDATARIKTGGDFS"
     gene            complement(2861..3523)
                     /locus_tag="PLD15_10195"
     CDS             complement(2861..3523)
                     /locus_tag="PLD15_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPB64322.1"
                     /translation="MKLIVDNVRRLRAEIPDYVTLIAATKTRSVQEILELLDSGVIDL
                     GENYVQEAQEKHSAIGIKARWHCIGHLQTNKVKKAVEIFDIIQTVDSVKLAVEIDKRA
                     GAIGKKMPLLIEINSAREENKSGVYPEKSPELLEEISHLENIIVVGLMTMGPVTDDVE
                     GIRPHLRLTRRLFEEIGTSGIENVEMRYLSMGMSNSYRVAIEEGSNMVRLGTIIFGER
                     KY"
BASE COUNT          934 a         1003 c          866 g          986 t
ORIGIN      
        1 gagacagctt ttcagtctcg agaccctttc tcgaatactc ctcgagcgct tcatcaagcg
       61 tttcgggaga gatgttgtag gtcttttcgt cgatatcggc gaagaccggt atggctccct
      121 cgtatatagc gacattcgca gaagagacga acgtgaaggg gctggtcagc ataacctttc
      181 ccctgtccag accgacggac ctcacgataa gatggagagc gcttgtacca ctgttcacgg
      241 cgatcgcata tttcaatccg acataatcgg cgatgatcct ctcgaattcc actaccttcg
      301 gacctataga caggattccc gagtccatga gaccggcaac ggcctcgatt tctctctctg
      361 tgatatcggg cctggcaagg ggaaccagca attactccac ctccctggta gaacgcgcaa
      421 aagttgatag aagaagacct aacatgaacg accatagggc cgcaagccct atctgataat
      481 cttttggaag catgaaagtg accgtgtggg ccggtatcca gaaccaaaga agcgagagaa
      541 aggccgaatc gagcttcttc cagttcctct tgcgttcaac gatgttgtct agaagtctgt
      601 ggaaaagaac gagaaagaca ccgaattgaa ggttcatggc caccgaacgc gccagagcgt
      661 tgaggaaagc tttttcgaag aagacctccg gcaaaactct gttgagaacg agagtgttga
      721 caaacccctc gaaacctatg aaggccactt ttatgaagat cgagagaacg gcccagacca
      781 gcatcttcga cagaaagagc gacatggaga cgctctcctt cgatcccgaa agtttcttgc
      841 tgacaagatc gcccagggtg ccgagaacgg cgaattgaat catggccgat atgaagggat
      901 agcctctcac gaagttcaaa taccagttca tgaaaaatct ccccccgtct tgattctggc
      961 cgtcgcatcg ggcataaatc cttgtattat tccgttcagg actgcataat cgctgttacg
     1021 tattgcaagc tcgagcttcg aaaccaactc ttccatctct ttttcaccga gaacggtctg
     1081 cccgttcaat ttgaagattt tgggatgttt agtcttcgat agggcctcgt cggtcatgac
     1141 tagctcctcg aaaagcttct cccccggcct ggctccggtg tactctatca ttatctcctg
     1201 ctgtggcacg taaccggcga gagtgatcat ctccctcgcc agagtcgata tcttgaccgg
     1261 ctcgcccata tccagaacga agatgtcgcc tccggcggcg tatgcgcctg cctggagaac
     1321 aagagaaacg gcctcgggta tggtcatgaa atatctggtc attctcggat cggtaacggt
     1381 taccggacca cccgactgta tctgcttttt gaagaggggt atgacactac cacggctgcc
     1441 gagaacattg ccgaacctca ctatcccgaa gcgcgtggcg ctgttttccg cgatcgacct
     1501 gacgaactcc tcggccagcc ttttcgaagc gcccatgata gaggaagggt tgaccgcctt
     1561 gtcagtcgaa atcatcacca tggcctcggc cgagtatctg tgagccaacg aggcaacgtt
     1621 tagagagccc agagaattca cccggaaggc ctcagtgggg ttttcctcca taagcgggac
     1681 gtgtttgtgt gcggccgcgt ggaaaatcag cgacggtctg tgagtcttga agatatactc
     1741 catgcggtct gtatcctgga catctcctat aagctgtacc acagggatat ccggatattg
     1801 cttcgaaatc tcctgcttta tttcatagat gctgttctca cctttgccca gaagaagcaa
     1861 ctttccggga ccaagcggag ctatctgtct gcagagttcg ctacctatgc tacctccggc
     1921 gccggtcaca agaactactc tttcgactat gtagtctttc aacgaggcca gatcgacctt
     1981 tacagactct ctgccgagca agtcttctat atctatctcc cgaaggtagc ctagcctggc
     2041 cttaccgtct attatctcgg tcaggctcgg aagagtctgg acctttattt ttttggtatc
     2101 gatgtaggag aagatcttcc tcagagtctg cgagtcggcc gagggtatgg caactattac
     2161 cttgccgatc ttgaatcctt cgaccagttc cattatccgc gtcgaatcgc caagaacctg
     2221 aataccccta attcttcggc ggagctttct gggagagtcg tcaacaaaac ccactactac
     2281 gccgtgttct ggatggcggg ccagttcttc ggcaagagat gttccggcgt ctccggcacc
     2341 cactatgagc actctttccg atgctgcaag tgcatggtgc cccctgttga taaagctcaa
     2401 ccacattatt ctcgaccata ttataagaaa ggccgaaccg atagcggcca tgcttgaaac
     2461 agtgaaaggg accgttaaaa gctttatgtt caagagaatg agtagccttc cgaccccaaa
     2521 tccggctacg aaacctgtcg caattccccg gatgacgatc cagtagtctc tgaaatagct
     2581 gtaactccaa gctattttgt aggtgccgtt caaggccaac gatataacta tgaaaagact
     2641 gaaaaaaatg ccgttcacga agaattccgg ctcgtacaga ttgaatatac cgtacctgaa
     2701 gtacatcccc ataacgtaag caaggtatat gagaagaaaa tcgagaagga gaagcgccac
     2761 atttctcctc atgccggtca gagctttcaa cttgaacaat acattcacct cggaagagat
     2821 tctagcacaa cagctacgga acggggaatc ggaactgagt tcaatacttg cgctctccga
     2881 atatgatcgt tcccagtctc accatgttcg atccttcctc tatcgcaacc ctgtaagaat
     2941 tggacattcc catcgaaaga tatctcatct ccacattctc gatccccgag gtacctatct
     3001 cttcgaataa ccttcgagtg agtctcagat ggggtctgat tccttcgacg tcatctgtca
     3061 ccggccccat ggtcataaga ccgacgacga tgatattttc gagatgggag atttcttcta
     3121 gcagttcagg ggatttttcg ggatatacgc cgctcttgtt ctcttctctg gcgctgttta
     3181 tttctatcaa caggggcatt ttctttccga tcgctcccgc tctcttatcg atttcgacgg
     3241 ccagtttcac ggaatcgaca gtctgaatta tatcgaatat ttcgaccgcc tttttcactt
     3301 tgttagtctg aaggtgacct atacagtgcc agcgggcttt aatgcctatc gcactatgct
     3361 tttcctgtgc ctcctgtaca tagttctctc caaggtctat gacaccgctg tcgaggagtt
     3421 ccagaatctc ctgaacactc ctggtcttgg ttgcggcaat aagtgtaaca taatcgggaa
     3481 tctctgccct caatcttctc acgttatcta cgatcaactt cacaacctcc cgatcaatta
     3541 tatatttgtt ccggtgactt ctaccgtaag tctgtcacta ttttcatgtt attattccgt
     3601 tgcggagatc catacaaacg aaaggaggtt ttattccgtg aaatcgagca ttatatttat
     3661 ttttgccctc ttgatcgcca tactcatatt ctcggtgttt ttattcattc aagacagact
     3721 cgccttgaaa gaacttcttc tatcggccag caagctggaa gaaagtgttt ccactctcca
     3781 gacgcgcat
//
LOCUS       DAOOLY010000238         3786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29282_length_3786_cov_4.234522, whole genome shotgun sequence.
ACCESSION   DAOOLY010000238 DAOOLY010000000
VERSION     DAOOLY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29282_length_3786_cov_4.234522"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLD15_10200"
     CDS             <1..1153
                     /locus_tag="PLD15_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPB64323.1"
                     /translation="SIVNFSAGLLEHFMAQRPDGSVPYSFESNKIDLSGSEKVVVFVI
                     DALGYFNLLRIMEENSFRLFSREDIRILTSVFPSTTTAALTSIFTANTPSRHGFLGYL
                     LNIPEYGGLVNMIELTPYTQDRDNLTRLGFDPLKYIERQTIFESLKEAGVRGYHLTSK
                     SFVNTGLTRMHTRGGIARGAHGIGDILEELKIVLDSEEASSLTIVYWGLIDTYGHRYG
                     PESLAYSVETTALIRSIEDFFDRYVEPGTSFFITADHGQIQTPWDREIWWSKFDRSFE
                     HMYSMPGGEQRMAYIYTSDREKTREVLEELYGDSLLVIPTDSLDWKRYFGGEMSNTLK
                     KRVGELITISKEDYSFCFKYTGQEHSLKGRHGGLSPEEMYVPLIFLKKG"
     gene            complement(1150..1800)
                     /gene="tsaB"
                     /locus_tag="PLD15_10205"
     CDS             complement(1150..1800)
                     /gene="tsaB"
                     /locus_tag="PLD15_10205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPB64324.1"
                     /translation="MKYLIVDTSSRTLLLSASNGKGDINLSLKDLDRHGSMLAVVIKQ
                     SLDLLEISPADLDFLGAGVGPGSLTGLRVGLSTIKGLSLPHNIPVAPFNSLGPLAEGM
                     DFDDFVICRKGREGHFYWRAYLKKRPGETLFSSTAEIKGKLDKSTIVCSERTEELADF
                     KEYRTFLVEPSPTSMQKVALEAFRSGKILSGIDLEPVYLQKSVAELNWDRRNPRKT"
     gene            complement(1808..2671)
                     /locus_tag="PLD15_10210"
     CDS             complement(1808..2671)
                     /locus_tag="PLD15_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPB64325.1"
                     /translation="MTALRKTLLDYSLITIGSILTSVAIVSFMIPNNIIAGGVSGLAI
                     ILYRIFGFWVGAQMLVYNIFLFALAFVVLGIGFGIKSIYSAILMSLTVDIMQKLHFPY
                     LDAASTQDGFLLAAIYGGVVAGVGMGLVLWRGASTGGTDIVAMILAKYFSFSTGSGLF
                     ISDSLITIFAIVVFGPIAAMYGIITIFTTGKTIDAIIEGIGNTRTAFVVSVNSTEIKD
                     RIISEMERGTTMIKATGGFTGEERPVLMVSLRRREIGQLRHIVKAIDKKAFVILVNNS
                     EVFGEGFKNLN"
     gene            complement(2668..>3786)
                     /gene="dnaJ"
                     /locus_tag="PLD15_10215"
     CDS             complement(2668..>3786)
                     /gene="dnaJ"
                     /locus_tag="PLD15_10215"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPB64326.1"
                     /translation="SRKDYYEILGVPRTATPEDIRRAYRKLVKEWHPDAYKGEDRKSA
                     EAKFKEIQEAYEVLSDQEKRAMFDRFGYVGDPSAYSRGSGRTPGAEGGHFDDIFGDFQ
                     DVFDVFFGSNRSGGASRTQGPRAIRGEDIHASVVVDLKDVILGKKVILEYDRSTTCKS
                     CNGNGSESGTSYRTCPNCNGTGYIKEEHRSFFGIFSNTHACNTCGGSGKITDKRCPDC
                     GGRGTQKERHQVSVNIPAGVENGSTLRIGGHGNAGQNGGPSGDLYVSVRVEMPSEYRR
                     NGNDLYVSKEIDYVEAALGTSVDVGLPEGGSETLKIPAGTSPSTVFRMKNLGVPNISG
                     SKRGDLLVTVRVNIGKPSSREKKLLQQIAKLKNSKVVE"
BASE COUNT          877 a         1003 c          938 g          968 t
ORIGIN      
        1 ttcgatagtg aatttctcgg ctggccttct ggaacacttt atggcccagc gccccgatgg
       61 tagcgttccc tactcatttg aatccaacaa aattgacctt tcaggatcgg agaaggtagt
      121 ggtcttcgtg atcgatgcgc tagggtactt caatctcctg aggataatgg aggaaaactc
      181 tttcagactc ttctccagag aggatatcag aatactcaca tcggtctttc cctcgaccac
      241 cacggcggcg ttgacatcga tattcaccgc aaacacccca tccaggcacg gttttcttgg
      301 gtatctgttg aacatacccg agtacggtgg tctggtaaac atgatagaac tgactccata
      361 cacacaggac agagacaatc tgaccagact gggtttcgat cctttgaaat atatagagcg
      421 gcaaaccatc ttcgaatcgc tgaaagaagc cggtgtcaga ggttaccacc tcacttcgaa
      481 gagcttcgtg aacacaggat taaccagaat gcatacccgc ggcgggatcg ccaggggagc
      541 gcatgggatt ggagatatac tcgaggaact gaagatagtt ctggattctg aggaagccag
      601 cagtctcaca atagtttact ggggcttgat agacacctac ggtcacagat atggcccgga
      661 atcgctggct tattcggtcg aaacgacggc tttgatccgc tccatagaag attttttcga
      721 taggtatgtg gagccgggaa cgagcttttt catcaccgcc gatcacggcc agattcaaac
      781 gccatgggac agggagatct ggtggtcgaa gttcgataga tcttttgaac acatgtattc
      841 gatgcccggt ggtgaacaga gaatggccta tatatacacc agcgatcggg agaagacgcg
      901 ggaggttctt gaggagcttt acggcgattc tctcctcgta attcctacgg acagcctcga
      961 ctggaagagg tattttggcg gtgagatgtc gaacactctc aaaaagagag tcggagagct
     1021 gatcaccatt tcgaaggaag attactcttt ctgtttcaaa tacaccggcc aggaacactc
     1081 actaaaaggc cgtcacggtg gattgtcgcc cgaggagatg tatgttcctc tgatatttct
     1141 caaaaagggc taagtctttc ggggatttct cctgtcccag ttgagttcgg caacggactt
     1201 ttgaagatag acgggttcaa gatcgatgcc ggaaagtatc ttcccgctcc tgaaagcctc
     1261 cagcgcgacc ttttgcatcg aagtgggcga cggttccacg aggaaggttc tatattcttt
     1321 aaaatccgca agttcctcgg ttctttccga acatactatg gttgacttat ctagcttccc
     1381 tttaatctcc gccgtagagc tgaaaagcgt ttcaccgggt ctcttcttga gatacgcccg
     1441 ccagtagaag tggccctctc tgccctttct gcagattacg aagtcatcga aatccatccc
     1501 ttcggccagc ggaccgagtg aattgaaagg agccacgggg atgttgtgcg gcagggagag
     1561 cccctttatg gtagacaacc ccactctcag accggtcaac gatccgggac cgacgcccgc
     1621 tccgagaaag tccagatcgg cgggggagat ctccagtaga tcaagagact gcttgataac
     1681 aacggccagc atggatccgt gacggtccag atctttcaaa gaaaggttga tatcgccttt
     1741 gccgttgctg gccgaaagca gcagggttct ggatgaggta tcgacaatca gatatttcat
     1801 acgcagttca attgaggttt ttgaagcctt cgccgaagac ttcggagttg ttgaccagaa
     1861 tgacgaaggc ctttttgtcg atggctttga ctatgtgtct caactggcca atttcgcgcc
     1921 gtcgtagcga aaccatcagt acgggccgct cttcgcctgt gaatccacct gtggctttga
     1981 tcatcgtggt tcccctttcc atctcgctta ttatcctgtc ctttatctcg gtgctgttta
     2041 cactcactac gaaggccgtt ctcgtgtttc ctataccctc gatgatcgcg tcgatagttt
     2101 tgccggtcgt gaatatggta attattccgt acatggcagc tatgggaccg aagacgacga
     2161 tcgcgaagat agttatcaac gagtcgctta tgaagagccc ggatccagtg ctgaaactga
     2221 agtactttgc cagtatcatg gcaacgatat ccgttccgcc cgtggaggct cccctccaaa
     2281 ggaccagtcc cattccgact cccgctacca cccctccgta gatcgcagcg agtaggaagc
     2341 catcctgagt ggaagcggcg tccagatatg gaaaatgtag cttctgcatt atgtctacgg
     2401 tcagcgacat aagtatcgcg gagtatatgg actttattcc gaagccgatt ccgagaacca
     2461 cgaaggccag cgcaaacagg aaaatgttgt aaaccagcat ttgggcgcca acccagaaac
     2521 cgaaaattct gtaaaggatt atcgccagac cgctaacccc gccagcgatg atgttgtttg
     2581 gaatcatgaa cgagacgatc gccacggacg tcagaatcga gcctatcgtt ataagcgaat
     2641 aatcaagcag ggtctttctc agagccgtca ttcgaccacc ttggagtttt tcaacttggc
     2701 aatctgctgc aacagcttct tttctcggct tgacggtttt cctatgttga ccctcaccgt
     2761 gaccagcaga tccccgcgtt tgctacccga tatgttcggg accccgagat tcttcattct
     2821 gaagacggtg ctcggactgg tgccggccgg tattttaaga gtctcgctac caccctcggg
     2881 aagtccgacg tcaaccgaag ttcccagggc ggcctcaacg taatctattt ctttactcac
     2941 gtagagatcg ttaccgtttc tgcggtattc ggaaggcatt tcaaccctta cgctcacata
     3001 aagatcgccc gacggaccgc cattttgacc ggcgtttccg tgccccccga ttctcaaagt
     3061 ggagccgttc tcaacaccgg cgggaatatt gacgcttacc tggtgtctct ccttctgagt
     3121 tcctcttccc ccgcaatcgg ggcagcgttt gtcggttatc ttcccgctgc cgccgcaggt
     3181 attgcaggcg tgtgtgttgc tgaagatacc gaagaaagat cggtgctctt cctttatata
     3241 gcccgttccg ttgcagttgg gacaggttct gtaactggtg ccgctctccg atccgttgcc
     3301 gttgcagctc ttacaggtgg tgctcctgtc gtactcaagg atcactttct ttcccaggat
     3361 tacatccttc agatccacaa ctaccgaagc gtgtatatcc tcgcccctga tggcgcgtgg
     3421 cccctgagtt cgcgaagctc cgccagatct gttacttccg aaaaagacgt cgaagacgtc
     3481 ctgaaagtct ccgaagatat cgtcgaagtg acctccttca gcgcctgggg ttctgcctga
     3541 gccacggctg taggccgaag gatctcctac gtaaccaaaa cgatcgaaca tggctcgttt
     3601 ctcttgatcg gaaagaactt cataggcttc ctgtatctcc ttgaattttg cctcggccga
     3661 ttttctatct tcacccttgt aagcatcggg gtgccattcc ttcaccagct ttctgtaagc
     3721 ccttcttata tcctccggcg ttgccgttct gggaaccccg agaatctcgt aatagtcctt
     3781 cctcga
//
LOCUS       DAOOLY010000239         3785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29294_length_3785_cov_3.933244, whole genome shotgun sequence.
ACCESSION   DAOOLY010000239 DAOOLY010000000
VERSION     DAOOLY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29294_length_3785_cov_3.933244"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLD15_10220"
     CDS             complement(<1..269)
                     /locus_tag="PLD15_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB64327.1"
                     /translation="MKRLALFVIVIIGITAFATSISYDPQGLMFLSRPGAGINVTVNL
                     NKGTGATYYGGETLGISFSVDRSAYVAVLDIDPSGQVQVLFPNVY"
     gene            complement(356..1399)
                     /gene="ispG"
                     /locus_tag="PLD15_10225"
                     /gene_synonym="gcpE"
     CDS             complement(356..1399)
                     /gene="ispG"
                     /locus_tag="PLD15_10225"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPB64328.1"
                     /translation="MSKAVKVGPVTIGGGNPVSVQSMTNTKTVDAQSTLEQIERLAGV
                     GCEIVRVSVPDNDSAVALKSITRESRLPVVADVHFDYRLAIESIRNGAAKVRINPGNF
                     GQEWKIIETVKVAEEYGVSIRVGANSGSIDQDFSHIGRSAALAESALAQVRLLEKAGF
                     EDIVISVKASRVRETVEATRYVSEKVDYPLHLGVTEAGFGLDSIVKSSIGIGALLLNG
                     IGDTVRVSISGDPIQEVEAAFSILRALNLRPGPDIISCPTCARTEINVEELAREVKQW
                     LKGIDDDITVAIMGCVVNGIGEGREADIGIAGTRAGGVIFMKGRVVEQTARGELEERF
                     KYWLDKAMGKGEN"
     gene            complement(1399..2910)
                     /locus_tag="PLD15_10230"
     CDS             complement(1399..2910)
                     /locus_tag="PLD15_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB64329.1"
                     /translation="MFLTIIYFLLILTGVVVVHELGHYLFSRLFGVRVIEFAIGMGPK
                     IWSRKGKKTTFRINAFPIGGYVRPAGEDLDAVDSSIPENEQIQNKPAWQRFIIYLAGP
                     AFSLILGFVILSLVAVVWGFQEIKIDKIEPGSPAEISGIAPGDRIVSVNGKILIDNTR
                     LGEAIAKGDPIDLVIMRDGREMGITLVPEMLPQETMMIISRAQGGAGGEIASINGATF
                     SGDYVGYGKVLQSESSIKIGFSDGQTLSGTLLNYSPAEERYAMGIYYANFQPRIAKDH
                     GLFKKGDMIVSVGSLETKTSYDLTMMSQLLAIDPSDLLIQFTGREVAYQNHGFAEEIP
                     VKLERDGKSVEITVGKQEMISLVSEAGVFSQGSDYWYPDSVFQAAGLGLQWANSILVT
                     MVRVVGSLFTGGANINEFTGPIGLVTIVDQAISLGLRIVIFITGFISLNLGVVNMIPF
                     PALDGGRMLLAILEMITRKRLDPKIEGLINVIGFMVLMGLMIYITFVDIGRWF"
     gene            complement(2901..>3785)
                     /locus_tag="PLD15_10235"
     CDS             complement(2901..>3785)
                     /locus_tag="PLD15_10235"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPB64330.1"
                     /translation="LESSRPDMVIVGVSGAAGLRFSLKAIEICKRLCLANKESIVCGG
                     DLLIEGCKEKSVELIPVDSEHSGLFQLLGEGPRPDRIFITASGGALRDWSVERSIDAT
                     PGDVLKHPVWSMGRRITIDSATMVNKGLEVIEAHYLFRYDEEDIETYICRNSYIHAGI
                     VYRDGVVKLHSGTPDMRIPIAYSLTYPFRERVIDRGGITGIPLLLEEIDGDKYPALEL
                     ARNICGKQSKQIAYNAADEIAVEAFLRGEIKFGRIYNIIERTVSRIDDQRRADYRQIL
                     EIDKISREIALEEVSKCF"
BASE COUNT          864 a         1080 c          835 g         1006 t
ORIGIN      
        1 tcatagacgt ttggaaacag aacctgtacc tgaccggaag gatcgatgtc cagaacggcc
       61 acatacgcgc ttctatcaac ggaaaaggaa attcccaggg tttcgccacc gtaataggtc
      121 gctccggtac ccttgttcag attgacagta acgttgatac ccgcacccgg tctgctaagg
      181 aacatgagac cttgaggatc gtaactgatc gaagttgcga aggctgttat accgataata
      241 acgattacaa agagcgcaag ccttttcatt ctctttcctc ctcctttatt gctgcggcaa
      301 tttgcctgca gacttgatta acgcgtttaa cggcaacgtc ggttttcttc cacaatcagt
      361 tttcgccctt acccatagct ttatcgagcc agtatttgaa cctctcttca agttcccctc
      421 tagccgtctg ttcgacaaca cggcctttca tgaagattac tccgcccgca cgggttccgg
      481 cgatccctat atcggcttcc cttccctctc ctataccgtt gacgacgcag cccattatgg
      541 ccacggtaat atcgtcgtcg atgcctttga gccattgctt gacttctctt gccagttctt
      601 cgacgtttat ttccgttctg gcacaggtcg gacaggagat tatatccggt cccggcctga
      661 ggttgagcgc tctgagaatc gagaaggcgg cctctacctc ctgtatcggg tcgcccgata
      721 tggacactct tactgtgtcg cctatcccgt tcagcaggag cgcacctatg ccgatcgaag
      781 acttcactat cgagtccaat ccgaatccgg cctccgtaac ccctaggtga agcggataat
      841 ctaccttctc actcacatat cgcgtcgctt ctacagtctc tctcacacgg gaggctttca
      901 cggagatgac gatatcttca aatccagcct tttcgagcaa cctcacctgt gccagggcac
      961 tctcagccag ggcggcactt cgaccgatat gcgagaagtc ttgatcgatt gagccggaat
     1021 tggcccccac tcttattgaa acgccgtact cttcggcgac cttcactgtc tcaattatct
     1081 tccattcctg tccgaaattc cccgggttga tcctcacctt cgcggctcca ttccttatag
     1141 attcgatggc caggcgataa tcgaaatgta cgtcggcaac gactggaagc cgggattccc
     1201 gtgtgatact tttcagggcg acagcgctgt cgttgtctgg aactgacact ctcactatct
     1261 cgcagcccac accggccaac ctttcgatct gttcaagagt ggactgtgca tctacggttt
     1321 tcgtgtttgt catcgactgg accgaaacgg ggtttccacc accaatagtt accggaccta
     1381 cttttacagc cttgctcatc agaaccagcg acctatatcc acgaaagtta tgtagatcat
     1441 caggcccatc agaaccatga aaccgataac atttatcaac ccttcgattt tgggatcgag
     1501 cctctttctg gttatcatct ccagaatggc aagcagcatc cttccgccgt cgagagccgg
     1561 aaagggaatc atgttgacca cgcccaggtt caaagatatg aagcccgtga tgaagatgac
     1621 tattctcaaa cctaggctta tagcctgatc cacgattgtc acgagaccta tcgggccggt
     1681 gaattcattt atgttggctc cgcctgtgaa gaggcttccc acgaccctga ccatggtcac
     1741 aagaatactg ttagcccact gtaaacccag accagcggcc tgaaagacac tgtcgggata
     1801 ccagtaatcg ctcccctggg aaaagactcc ggcctcgctc acgagcgaaa tcatttcttg
     1861 cttacccacg gtgatttcga ctgattttcc gtccctttcg agcttcacgg gaatctcttc
     1921 ggcgaaaccg tgattctggt aggctacctc ccttccggtg aactggatca gaagatcgga
     1981 tggatctatc gcgagcagtt gactcatcat ggtaagatcg tagcttgtct tcgtctctaa
     2041 agaacccact gaaactatca tatctccctt tttgaagagc ccgtggtctt tggcgattct
     2101 cggctggaag ttggcgtagt aaatccccat ggcgtatctt tcctcggccg gcgagtaatt
     2161 gagcagagta ccggacaggg tctgtccatc ggaaaaaccg attttaatgg aactttccga
     2221 ctgaagcact ttaccgtagc ccacatagtc accgctgaat gtggctccat taatcgaggc
     2281 gatctctcca cctgcgcccc cctgagcgcg ggagataatc atcatggtct cctgtggaag
     2341 catttccggc acgagagtga ttcccatttc tctgccgtct ctcattatca ccagatctat
     2401 cgggtcacct ttcgcgatcg cctctccaag ccgcgtgttg tcgatcagta tcttcccgtt
     2461 caccgagact attctgtccc ccggcgctat gcctgaaatt tcggcaggag atcccggctc
     2521 tattttatcg atcttgatct cctgaaaacc ccatactaca gcgacaagcg ataaaatgac
     2581 gaaaccgagt atcaacgaaa aggcaggccc ggcaaggtat attatgaagc gttgccacgc
     2641 gggtttattc tgtatctgtt cattctcggg tatcgatgaa tcgacggcgt cgagatcctc
     2701 tccggccggt ctgacgtagc cgccgatggg aaaggcattt atcctgaaag tggttttctt
     2761 gcctttcctg gaccagatct tcggtcccat gcctattgca aactctatca ccctaacacc
     2821 gaaaagtctc gaaaaaaggt agtgccccag ttcatggact acgacgaccc cggttaggat
     2881 caaaagaaag tagattatcg tcaaaaacat ttgctcacct cttccagcgc gatttctcgg
     2941 gaaatcttat cgatctccag tatttgacgg tagtcggccc gtctttgatc atctattctg
     3001 gagaccgttc tttcgattat attgtaaatt cttccaaact ttatttcacc tcttaaaaaa
     3061 gcctcgacgg ctatttcgtc ggccgcattg taggctatct gtttcgactg ctttccgcag
     3121 atgttccgtg ccagttcaag cgcggggtac ttatcgccgt cgatctcttc cagcagtagg
     3181 ggaatccctg tgatcccccc tctatcaatc acccgctccc tgaaaggata agttagcgag
     3241 taggcgatcg gaattctcat atccggcgtt ccactgtgca gtttcacgac cccgtctctg
     3301 taaactatac ctgcgtgtat atagctgttt ctacatatat aggtctcaat atcctcttca
     3361 tcgtatctga acagataatg cgcttcgatc acttccagac ccttattaac cattgtagca
     3421 ctgtcgatgg tgatccttcg ccccatcgac catactggat gtttcaagac atctccagga
     3481 gtggcgtcta tagatctttc aacgctccag tctctcagag ctccgcccga ggccgtgatg
     3541 aatatccgat ctggacgggg tccctcgccg agaagttgaa agagaccgct gtgctccgaa
     3601 tcgacgggta tcaattctac agatttctct ttacatccct cgatcagaag atctccgccg
     3661 caaacgatcg attctttgtt ggccaggcag aggcgtttgc atatttcgat cgccttcagg
     3721 ctgaaacgta aacctgccgc accgctaacg cccacaataa ccatgtctgg ccttgaactc
     3781 tccag
//
LOCUS       DAOOLY010000240         3776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29395_length_3776_cov_4.457941, whole genome shotgun sequence.
ACCESSION   DAOOLY010000240 DAOOLY010000000
VERSION     DAOOLY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29395_length_3776_cov_4.457941"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLD15_10240"
     CDS             complement(<1..340)
                     /locus_tag="PLD15_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPB64331.1"
                     /translation="MRKNKFVLILTLVAFCVISLASLKDVSTSPFSTAIEKLYASGIV
                     EVEDGLFNPSDTLSRAEAAEWIVKTFKLSTIHPMIIEEEVQKKFVYTDPLGVIDEGFV
                     VPSAKDTVGID"
     gene            638..1726
                     /locus_tag="PLD15_10245"
     CDS             638..1726
                     /locus_tag="PLD15_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPB64332.1"
                     /translation="MRFEIVKKYMDKRCIDAVLLDSPDSICWYFEGEVDSRIDQTRER
                     GSFSILITGNKTIAICPEYDYERLKDEVLPPEVDAISCGRGEEFDHAVKRFCRSFEKV
                     STDNRRMYDERFSLLDEEFYLNMQELNDREIMRLRIMGKLSESILCEFAPELQPGMTE
                     LEIEKVLRALLIESGLEVPVLCVGSDERVARYVYPVSTGKKVQKYIVMRAVVRKQGLR
                     IAFTRYFHFGEIPSEIEQNHERASGVFAKAAVAMMKARSMGEIYEEIQRAYSELKITP
                     DFNGCPPGTSLGYSGEKFEMSQNSSLPVRSPAAYVLSPRVGGIFSEDTILVSDGGTAE
                     FVTLSEDFPKIKVRLESFRVHRPWIMVL"
     gene            1877..3355
                     /locus_tag="PLD15_10250"
     CDS             1877..3355
                     /locus_tag="PLD15_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB64333.1"
                     /translation="MQEYVDFTRPEYKKTRKLAILEGSFYNMAFLVTQGFIVTGLALE
                     YGVSEFLIAIIGVLPTLAQLTQLISPIFFRLFKSRKKSMVFAAIIGRVPLAFIPLTLA
                     LGIKVQSLLLVILAMVSLGNSLVGTFWASIMRDVIEPSSTGKYYGRRNLILSLSSMLI
                     TPLYSLILDTFEGDMGFIIVTVIATCFAMLTIILLMNHYNPPVRTYGSGRMFKEVFSN
                     LKFRQYLKFSMLWNFAITVSGPFYSYHQLVNLKVSYSYLSVLSIVASLTSMVMYILWG
                     KISDQIGHQSVAEFGILGATCLALMWTFVTPQSYSILLPVDAIVTGMVWSAINLCIFT
                     MMMGMMKGLSVEPYFAVQAFLNGLGALGGSLLGGVIASFLKGKVIYLLGMEFHGIQAI
                     FLLGSMLRLNAFFMLRKVQTPKIKSVSQLFFNVMSTVGRRMATRPYEYPMLLMGAGRK
                     KSEKEHLPLDLPESLKEPGDDIVVEPSTGADPEDRSGLEPHE"
     gene            complement(3356..>3776)
                     /locus_tag="PLD15_10255"
     CDS             complement(3356..>3776)
                     /locus_tag="PLD15_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64334.1"
                     /translation="EKVISAAKYVYAHSFIEKLSNGYDTMILERGGTLSAGERQLIAL
                     ARAVVFDTDILVLDEATANVDTETEALIQKAMERISSEKTMITIAHRLSTIRNADRIM
                     VIHKGSLVESGNHEELLAKGGIYTDLYRLQYELGDTA"
BASE COUNT          966 a          834 c          944 g         1032 t
ORIGIN      
        1 cgtctattcc cacagtgtct ttggcggagg gtaccacgaa gccctcgtcg attacaccaa
       61 gcgggtcggt gtaaacgaat tttttctgca cctcttcttc gataatcatc gggtgaatgg
      121 ttgaaagttt gaatgttttc actatccatt cggctgcctc tgctctggaa agagtgtctg
      181 aaggattgaa aagaccatct tcgacctcta cgatcccgga agcatagagc ttctcgatag
      241 cggtcgaaaa tggactggta gatacgtcct tcaggcttgc caaagatatt acacagaacg
      301 cgaccagagt taagatcaga acgaatttgt ttttccgcat gttacccctc ctcttgatga
      361 agtgtcgaaa tatttatagt cacaatagaa tcggtctacc tatacctttt caaagatcca
      421 caccgcggtc cgatcgacta cttacagatc tttccggata atatcttcat aagtattata
      481 aagccttgga gtggtaagaa gtagtcaccg cttcgagatt aacctgaatc gatcgagcaa
      541 ttaggtgggt tcgcgaaaaa acagactggc tagaatatca ccaagaatga ctttctgata
      601 gaatggggac gagctttcat atggaggggt agtgttggtg cgctttgaga tcgtcaagaa
      661 atacatggat aaaagatgta tcgacgcggt tttgctcgac tctcccgaca gtatctgctg
      721 gtacttcgaa ggagaagttg attccaggat cgatcagaca cgcgagcggg gatctttctc
      781 aatcttgatt acaggcaaca aaacaatagc gatttgtccg gaatatgatt acgaaaggct
      841 gaaggacgag gtcctcccgc cggaggtgga tgcgatttcc tgtggcagag gtgaggagtt
      901 cgatcacgcc gtgaaaagat tctgcagatc attcgaaaaa gtctccaccg ataaccggag
      961 aatgtatgac gaaaggtttt cactccttga cgaagagttc tatcttaata tgcaggaatt
     1021 gaacgacaga gagataatga gattgcgtat catggggaaa ttgagcgaga gcattctctg
     1081 cgagttcgct ccggaactgc agcccggcat gacagaactg gaaatagaga aagtgctgag
     1141 ggcactcctt atagagtctg gtttagaggt tccggtccta tgcgtgggtt cagacgagcg
     1201 agtagccaga tatgtctatc cggtttcaac tggaaagaag gtacagaagt acatagttat
     1261 gagagccgtt gtaagaaagc aaggtttgag aatagctttc acgcgttact tccattttgg
     1321 agagatccct tcagaaattg aacagaacca cgagcgtgcc tcgggtgtct ttgcaaaagc
     1381 cgcagtggct atgatgaagg ccaggagtat gggagagatt tacgaggaaa ttcagagagc
     1441 ttactcggag ttgaagataa ctccagattt caacggctgt ccaccgggga cctctctggg
     1501 atattcggga gagaagtttg agatgtccca gaactccagt cttccggtaa gaagcccggc
     1561 tgcatatgta ttgagcccga gagtgggcgg aatattcagt gaagatacga tactcgtaag
     1621 cgacggtggg acggccgaat tcgttaccct cagcgaagac tttccgaaga taaaggttag
     1681 gttagaaagt ttcagggttc acaggccgtg gataatggtt ttgtgatttt ttgctctttt
     1741 aagtgtgagc tgtattcgag gttagtcctt ggtttgttaa agtaaaacac cattcaaagt
     1801 atcgtgacat tataccgttg gagaatgata aactctcagt gagagattca tttattatcc
     1861 gcggttggga gtaaagatgc aagaatacgt tgacttcaca agacctgaat acaaaaaaac
     1921 caggaaactt gcgatcctcg aaggttcttt ttacaacatg gccttcctgg tcacacaggg
     1981 tttcattgtt acaggtctgg cgctggagta tggagtatcg gagtttctga tagccataat
     2041 cggagtattg ccgaccctgg cgcagttgac ccagctaatt tcgccgatct tttttagact
     2101 cttcaaaagc agaaaaaaat cgatggtctt cgcggccatt ataggaaggg tgcctctggc
     2161 cttcataccg cttactctag cgctgggtat aaaagttcaa tcgttgcttc tcgtgatcct
     2221 agccatggta tctttaggca attctcttgt tggcactttc tgggcatcca taatgagaga
     2281 tgtgatcgaa ccgtccagca cgggaaaata ctacggaaga agaaacctga ttctctcact
     2341 ttcttcgatg cttatcaccc ctctctattc actcatactg gacactttcg agggcgatat
     2401 gggattcata atagtgacgg ttattgccac ctgtttcgcc atgttaacca tcatcctgtt
     2461 gatgaatcac tataacccgc ctgtgagaac ttacggtagc ggacgcatgt tcaaagaagt
     2521 cttctcgaat ctgaaattca gacagtattt gaaattctct atgctctgga acttcgccat
     2581 caccgtttca ggccccttct attcttatca tcagctggtc aacctcaagg ttagttactc
     2641 ctatctcagt gtacttagta tagtcgcttc tctcacttcc atggtcatgt acatcctgtg
     2701 ggggaagatc tccgaccaga taggccacca atctgtagcc gaattcggca ttctcggagc
     2761 cacctgtctg gcgttgatgt ggactttcgt gacaccccaa tcctattcta ttctgctacc
     2821 ggttgacgca atcgtcactg gaatggtctg gagtgccata aacctctgta tcttcaccat
     2881 gatgatggga atgatgaaag gtttgtcggt tgaaccgtat tttgcggtcc aggcctttct
     2941 caacgggctg ggagcgctcg gaggctcatt gctgggcggt gtaatagcct ctttcctgaa
     3001 ggggaaggta atttatctgc tgggtatgga gtttcacggt attcaggcta tctttctatt
     3061 gggaagtatg ttgaggctga acgctttctt catgctgaga aaggttcaaa ctcccaagat
     3121 caaaagtgtc tcgcagctgt ttttcaatgt catgtcaacc gtgggaagaa gaatggccac
     3181 caggccttac gagtatccga tgctcctgat gggtgcagga aggaagaaaa gcgaaaagga
     3241 acatctcccg ctagaccttc ccgagtctct gaaagaaccg ggtgacgata tagttgttga
     3301 gccttcgacc ggagccgatc ccgaggatag gagtggcctg gaaccacacg agtgatcagg
     3361 cagtgtcgcc gagttcgtac tggagcctgt acaggtcggt gtagatacct ccctttgcaa
     3421 gaagctcttc atggttaccc gattcaacaa gtgagccttt gtgaataacc attatcctat
     3481 cggcgtttct gatcgttgaa agtctgtggg cgatcgttat catagtcttt tccgatgata
     3541 tcctctccat tgccttctgg ataagcgctt cggtctcggt atcgacatta gccgtggcct
     3601 catcgagaac caggatgtcc gtatcgaaga ccacggctct tgccagcgct ataagctgtc
     3661 tttcgcctgc cgaaagagtt cctcccctct cgagtatcat tgtgtcgtac ccgttggaga
     3721 gcttttctat gaagctgtgg gcatagacgt acttggccgc cgatatgacc ttttcc
//
LOCUS       DAOOLY010000241         3758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29592_length_3758_cov_4.697543, whole genome shotgun sequence.
ACCESSION   DAOOLY010000241 DAOOLY010000000
VERSION     DAOOLY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29592_length_3758_cov_4.697543"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLD15_10260"
     CDS             complement(<1..312)
                     /locus_tag="PLD15_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64335.1"
                     /translation="MRKEWLLLKPDDESVKRLVEFLGIDTFLARLLVTRGIKDEIEAM
                     KFLNPDKTILHDPFILQDMTVAVRTIIGARDRDESIVIFGDYDVDGVTSTALLYLAMK
                     RM"
     gene            422..1120
                     /locus_tag="PLD15_10265"
     CDS             422..1120
                     /locus_tag="PLD15_10265"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB64336.1"
                     /translation="MRIDRYISKFEGLSRNYSRSLIKSGKVSVDGRVVRDPSLHVEEN
                     ANVTLDGSRVIAFGMIYIMMNKPAGYVCSREESEGPTVFDLVQERFSYDLSVAGRLDR
                     DSTGLTLLSNDGLFVHHVISPKKTIEKEYLVQTVKPVTPEQIEAMNGGLLIGRGEFSK
                     PVSVRTLGENSVEIILTEGRFHEVKRLMKASGNEVLTLHRSRIGGLSLPGSLKPGEWA
                     NIGEDALKIITGAE"
     gene            complement(1152..3182)
                     /locus_tag="PLD15_10270"
     CDS             complement(1152..3182)
                     /locus_tag="PLD15_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPB64337.1"
                     /translation="MKKALLVSFLIVMAVVSFGVVTYDLQKVIIVPEKPQGTLEVTIW
                     LDRDNGSLYYSGEEVKTYFKVNKDAYVAIYDITPNGEIQLIFPNGYDRDNFVRANRNY
                     TLPTDSASTRYRLQLTSETGGGKEIFQIVASTQPLGFLDDLLKKVQSGDIFPKASSSA
                     DDFVVSKVIPVIDKQEYAVSTAWFYLDIMPNKGYARITTTPSGAILYVDGKNIGTSPI
                     TLELDEGNHIVTAYRDGYKTETRQFTISAGRTVDIRIDLQKLVSNYQLSIATNPSSAD
                     VYIDNRYVGRSPLNLTLEEGTKSLRIERSGYETYYETFLLDRSLSKNITLKKIATNYQ
                     LSLITNPSGADVYIDNTYMGRSPLNLTLEEGTKNLRIERSGYETYYETFVLDRSLSKN
                     ITLKAIVTNYQLSLITNPSSADVYIDNTYMGRSPLNLTLSEGTKNLRIEKSGYETYYE
                     TLLLDRSISKSITLSPQIKTYKLSISSSPSNARVYINGTYEGMTPIALTLKEGTYTVQ
                     LTLDGYEDFVTSVNLDRDRQVTATLYSKKARLSIETDPSNATIYVDNSYVGRSPISID
                     LDAGRHTVKVEKAGYLTETRDVNLSAGTTSSLKVVMQEEKPVARINISSDPKNARIFI
                     NGKDYGRTDRIVELEPGYYEIILILDGYRISMTYRYFGKGDHNLSFNLTKID"
     gene            complement(3208..>3758)
                     /locus_tag="PLD15_10275"
     CDS             complement(3208..>3758)
                     /locus_tag="PLD15_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HPB64338.1"
                     /translation="AYQNGLELFRGVKLSLVALPGWLFLRGFLRSVKQKISGADLTVL
                     CLVAVGAVYYILRSGNFSLVLDLERRFRDVLDSLLLVRPRFKEILAYPLLAISIKNGF
                     KFEGKLGPLIVAGGSISVVSVVNTFCHATVPLWTGLLRSLYSVAFGVLFTLALNWFSK
                     KSGSNVKELEGSLNVESREESK"
BASE COUNT          905 a          895 c          934 g         1024 t
ORIGIN      
        1 cattctcttc atcgccagat aaagcaatgc ggtgctggta acaccgtcca catcgtaatc
       61 accgaagatc acgatactct cgtctctgtc tctcgcgccg ataatggtcc tgacggcaac
      121 cgtcatgtcc tggagtataa aaggatcgtg caggatagtt ttgtcggggt tcaggaactt
      181 catagcttcg atttcgtctt ttatgcccct tgtaacaagc aacctggcca aaaaagtgtc
      241 tattcccaga aactcgacga gtctcttcac agattcatcg tcgggcttaa gcagaagcca
      301 ctcctttctc ataaggaatc caccctctct tttttctttt gtaaagatta taccattgac
      361 cgcattaccc tggtttagca agcgcctggt taagcaagaa atcctggtgt agaatctgtt
      421 tatgagaata gatcgctata tttcgaagtt tgaagggctt agcaggaatt acagtcgatc
      481 gttaataaag agcgggaaag tctccgttga cggcagggtc gtgcgggatc cttcacttca
      541 tgtggaagag aatgcgaacg ttacgctcga cggaagcagg gtgattgctt tcggaatgat
      601 ttatatcatg atgaacaaac cggccggcta tgtatgctcg agagaggaat cggaggggcc
      661 gactgtcttc gaccttgtac aggaaaggtt ttcgtacgat ctctccgttg ccggaaggct
      721 cgatagggac tccaccggat tgactttgct gtcgaacgac ggtctgttcg tacatcacgt
      781 tatctctccg aaaaaaacta tcgaaaagga gtacctggtc cagacggtga agccggtcac
      841 gcctgaacag atcgaagcca tgaatggcgg tctgttaata ggccgtgggg agttttcgaa
      901 accggtgagt gtgaggacac tgggagagaa ttcggtcgag ataatcctta ccgaaggacg
      961 tttccacgaa gtcaagaggc tgatgaaggc ttcaggaaac gaggtgctga cacttcacag
     1021 aagtcgaatc ggcgggcttt cgctccctgg gtctctgaaa cccggcgagt gggctaacat
     1081 cggagaggac gcactgaaaa taataaccgg ggccgaatag ccccggttat ttctggatat
     1141 cgatcgagat ctcaatcgat ttttgtcaga ttgaatgaaa ggttgtgatc gccctttccg
     1201 aagtagcgat aagtcatcga tatcctgtag ccatcaagaa tgaggattat ttcgtagtac
     1261 ccgggttcca attcaactat cctgtcggtt ctcccgtagt ccttaccgtt tataaagatc
     1321 ctggcgttct tgggatcgga ggagatgttt attcttgcga ccggtttctc ttcctgcata
     1381 accaccttca gcgacgatgt ggtaccggcg ctcagattta catctctggt ctcggttaga
     1441 tagccggcct tttcgacctt gaccgtgtgt cttcctgcat cgagatctat ggatatcgga
     1501 gatcttccga catatgagtt gtcaacatag atggtggcgt tggaaggatc ggtttcgatc
     1561 gaaagtctgg cctttttgct gtagagcgtt gcggtcacct gtctgtctct atccagattg
     1621 accgacgtca cgaagtcttc gtagccgtcg agcgtaagct gaaccgtgta tgttccctct
     1681 ttcaacgtca aagctatcgg ggtcatgcct tcgtaagtac cgttgatata gactctggcg
     1741 ttcgaaggag aactcgaaat cgatagcttg taggtcttga tttgcgggga aagcgttatg
     1801 ctcttggata ttgaacggtc cagcagcaag gtctcgtaat aggtttcgta acccgacttc
     1861 tcgatccgca ggttcttggt cccttccgaa agtgtcaggt tcagcgggct gcgccccatg
     1921 tatgtgttgt cgatatatac gtcggcgctc gaagggttcg ttatcagcga gagctggtaa
     1981 ttggtgacga tcgccttgag agttatgttc ttggaaaggg aacggtcgag tacgaaggtc
     2041 tcgtaataag tctcgtaacc ggacctctcg atccgcagat tcttggttcc ctcttcgagc
     2101 gtcaggttga gcgggctgcg acccatgtac gtgttgtcga tatagacatc tgcacccgaa
     2161 ggattggtta tgagagaaag ctgataattg gtggcgatct tcttgagggt tatgttcttg
     2221 gaaagagaac gatcgagcag gaaggtctcg taataagtct cgtaaccgga cctctcgatc
     2281 cgcaaactct tggttccctc ttcgagcgtc agattgagcg gactgcgacc cacgtacctg
     2341 ttgtcgatat atacgtcggc actcgaagga ttcgttgcga tggacagctg gtagttgctg
     2401 accagcttct gaagatcgat cctgatgtcc acagttctac cggcgctgat ggtgaactgt
     2461 ctcgtttcag tcttgtaacc gtctctgtaa gccgtcacta tatggttgcc ttcgtccagt
     2521 tccagagtga tcgggctcgt tccgatgttc tttccatcga cgtacagaat ggcgccggaa
     2581 ggcgtggtcg ttatcctggc gtatcccttg ttcggcatta tatccaggta gaaccaggca
     2641 gtagaaactg cgtattcctg cttgtcgatg accggtatga cctttgaaac aacgaaatca
     2701 tctgcactgg agctggcttt cgggaagata tcgccgctct gaaccttctt gaggagatcg
     2761 tcgagaaaac ccagtggctg tgtcgatgcc actatctgga agatctcctt tcctccgcca
     2821 gtttcgctcg tcagctgcaa tctatagcgt gtcgatgcgc tatcggttgg aagggtgtaa
     2881 tttctatttg ccctgacgaa attgtctcta tcgtaaccgt tggggaagat caactgtatt
     2941 tctccgttgg gagttatgtc atatatagcc acataagcgt ctttgttgac tttgaagtac
     3001 gttttcactt cctcgccgga gtaatataga gatccgttgt cccggtcaag ccaaatcgta
     3061 acctcgagag taccctgagg tttttcggga acgattatca ccttctgtag atcgtacgtt
     3121 accactccaa aggaaacaac tgccatgact atcaaaaatg aaaccagcag cgcttttttc
     3181 atgttaaatc cctcctcact caagatgtta tttagactct tctctgcttt ctacgttcaa
     3241 agatccttcc agttctttca cattgctgcc gcttttcttc gaaaaccagt tgagcgccag
     3301 cgtgaacaat actccaaaag ccaccgagta tagactcctc agcagacctg tccagagagg
     3361 aacagtagcg tggcaaaaag tattaacgac tgaaacgacc gatatggaac caccggcaac
     3421 tatcaacggt ccaagctttc cctcgaactt gaaaccgttc tttatcgaaa tagccagaag
     3481 cgggtatgcc agaatttctt tgaaacgcgg tcttacaagt aacaggctat ccagaacatc
     3541 tctgaaccgt ctttccagat ccagcactaa cgaaaaattc ccgcttctca gtatgtagta
     3601 tacagctccg acagctacca ggcaaagaac cgtgagatct gctccactta tcttttgttt
     3661 tacacttcgc aagaaacctc tcaggaatag ccatccaggg agggctacga gggatagttt
     3721 tactccacgg aagagttcaa ggccattttg ataagccg
//
LOCUS       DAOOLY010000242         3750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29661_length_3750_cov_4.551286, whole genome shotgun sequence.
ACCESSION   DAOOLY010000242 DAOOLY010000000
VERSION     DAOOLY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29661_length_3750_cov_4.551286"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLD15_10280"
     CDS             <1..587
                     /locus_tag="PLD15_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HPB64339.1"
                     /translation="GACIVVCPAKAITLRENKSGDYYLSRFNGTPLVHALLYPGEETS
                     GGLVAEVRKLAIAISEEEKRGLVIIDGAPGIGCPATSSITGVSYVVVVSEPTVSGLHD
                     LERIIETVRHFRRDCGVVINKCDINREKTSQIENWCGANRIQVLGKILYDEKVGEATM
                     LGVPVVSLDGSGAADGIREIKNKLENIMISGGKR"
     gene            584..907
                     /locus_tag="PLD15_10285"
     CDS             584..907
                     /locus_tag="PLD15_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB64340.1"
                     /translation="MKIAVPVKEKSLDGFPDDRFARGKYFLIYDPDSKEKEFIDLSIE
                     AAHGAGPLAVNFLAGKGVDTIIAFHLGQNALQAIEAAGMAFFEARDGTVRENIERVLD
                     HRNKR"
     gene            970..1419
                     /locus_tag="PLD15_10290"
     CDS             970..1419
                     /locus_tag="PLD15_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV (family X)-like protein"
                     /protein_id="HPB64341.1"
                     /translation="MKREQLAAQFDLLSRLLRISRDNPFKIRSYEFAARTVRSRLSAG
                     ELTEQEIYDLSRLKGIGKAITEKSLEFLREGKISKISELGEAMPPAIFELALDGTVDP
                     ETIAFMWKDAGLGESESVLAFLKKQREDLKLSREELARLEERLKRGP"
     gene            complement(1426..2928)
                     /locus_tag="PLD15_10295"
     CDS             complement(1426..2928)
                     /locus_tag="PLD15_10295"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPB64342.1"
                     /translation="MSLIEELNSRYTELSFLNSAVALMAWDMRTYLPPMGNEMRAEAL
                     GYISTLAFKKRVSDELGSILENLRQPETYNSLNDEEKAIVRVSAKAFERARAIPPALY
                     QKFAVLTVRSEKAWEKARAENDFEGFAPYLEEIVSLSREMANLYGYAENPYDALLEGY
                     EEGMTAKRLREIIEPLKKELVPFLKRLMKEGKAPETDLLKGRFPRKTQEKLSHKALKF
                     IGYDFGAGRLDETVHPFTIGIGVGDVRVTTKYPPDEFTPSLFGSFHEGGHAIYEQGLP
                     AHLKGTSLYEAASLGIHESQSRMMENIVGRSKEFLKFFYPHIQKAYPSNFKNVPLKKF
                     YRSVNNVKPSLIRIEADEVTYNFHIMLRFELEEGLMKGDIQVEELPARWNEKMKEYLG
                     IEPANFSEGVLQDIHWPSGMIGYFPSYMLGNLYAAQFYAKAKSDIKDLERKIEKGDLK
                     PLLHWLRENIHGQGRRYSPGELLKVVTGKELDPGYFIKYVKDKYSNIYSL"
     gene            complement(2966..>3750)
                     /locus_tag="PLD15_10300"
     CDS             complement(2966..>3750)
                     /locus_tag="PLD15_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64343.1"
                     /translation="NIGLEGMILLSAFSGVVVSSATSSVVVGTLAAIATSIAVSLLIF
                     VMVRYMRANLYITGIATNLLSGGLTVMLADLWFRSKGTVVFPEAPRPAVSSLDFLGFL
                     NNLNFFDYLAIASPLLLLFVLRETSFGLKLIACGIDEESAYYSGVNVNRVRLVSFIIA
                     GVFSGLAGASLSLPLGLFVANMSGGRGWIALVAVIMGRDRPFLIGFSALMLGFVTELS
                     IFLQVSTQISPKLLLTIPYFVSFLIVAIFPDRRAMLEGKKHE"
BASE COUNT          934 a          952 c          969 g          895 t
ORIGIN      
        1 gtggagcctg tatcgtcgtc tgcccggcaa aggcaataac actcagagaa aacaaatccg
       61 gagattatta cctttccagg ttcaacggca ctcctctcgt tcatgccctc ctgtaccccg
      121 gcgaggagac ctccggaggt ctggtggccg aggtcagaaa gctggctatc gccatttccg
      181 aagaagagaa gagaggtctg gttatcatag acggagcacc gggaataggc tgtccggcga
      241 cctcttccat aaccggggtt tcctacgtgg tagtggtttc agaacccaca gtgtctggac
      301 tgcacgacct cgagagaata atcgagaccg tccgtcattt ccggagggat tgcggagtcg
      361 ttataaacaa atgtgacatt aatcgtgaaa agacctcaca gatcgaaaac tggtgcggtg
      421 caaatagaat acaggtactt ggaaagatcc tttacgatga gaaagtcggc gaggctacaa
      481 tgctgggtgt tccggtcgta tcgctcgacg gttccggagc ggcggatggt ataagagaga
      541 taaaaaacaa actggagaat atcatgattt ctggaggtaa gagatgaaga tcgcggttcc
      601 ggtgaaggag aaatcgctag acggctttcc cgacgatcgt ttcgcccgcg gaaagtattt
      661 tctgatatac gatcccgatt ctaaagagaa agaattcata gatctgtcga tagaggccgc
      721 acacggtgct ggccctttgg ccgtgaactt tctggcgggc aaaggcgtgg atactataat
      781 cgccttccac ctgggtcaaa acgctcttca agcgatagag gcggccggaa tggccttttt
      841 tgaggcccgt gacggaacgg tgcgcgaaaa tattgagagg gttctggatc acagaaacaa
      901 aaggtgagac cgacgtccgc cttgtctgta tgaccacttc cgggataaaa tagatccggg
      961 ggtgatgagt tgaaaagaga acaactcgcc gcgcagttcg atctgctgtc gagattgctc
     1021 agaatctcca gggacaaccc cttcaaaatc aggagctatg agttcgcggc aagaactgta
     1081 cgtagcaggc tttccgccgg ggagttgacc gaacaggaga tctacgacct ttcccggttg
     1141 aaaggcatag gcaaggccat aaccgagaaa tcgctcgagt tcctgcgcga gggaaagatt
     1201 tcgaagattt ccgaacttgg cgaggcgatg cctccggcca tcttcgaact cgctctagat
     1261 ggtaccgtgg atcctgaaac tatagccttc atgtggaaag atgccggtct cggcgaaagc
     1321 gaatctgtac tggcttttct gaaaaagcag agagaagatc tgaagttgag ccgggaagag
     1381 ctggccagac tcgaagagag attgaaaaga gggccctgag gccctctaga gtgaatatat
     1441 gtttgaatac ttgtctttga cgtactttat gaaatatccg ggatccagtt cttttccggt
     1501 aacgactttc agcagttctc cagggctgta acgcctgccc tggccgtgga tgttctccct
     1561 gagccagtgc aacaggggct tcagatcgcc cttttcgatt tttctctcga gatcttttat
     1621 gtcgcttttt gcttttgcgt agaattgtgc agcgtagaga tttccgagca tataagaggg
     1681 gaagtatcct atcataccag acggccagtg gatatcctgc agcacaccct cgctgaagtt
     1741 ggccggttcg attccgagat actctttcat cttttcgttc cacctggccg gaagctcttc
     1801 cacctgtata tcgcctttca tcaaaccctc ttcgagctcg aatctcagca ttatatggaa
     1861 gttgtacgtc acttcgtcgg cctcgatcct tatcaaggag ggcttaacgt tgttgacgct
     1921 tctatagaac ttcttgaggg gcacgttttt gaaattcgaa gggtaggcct tctgaatgtg
     1981 cggatagaag aatttcagaa actctttgct gcgtcccaca atgttttcca tcattctcga
     2041 ctgggattcg tgaataccca gagatgccgc ttcgtacaga gacgtaccct tcaaatgggc
     2101 gggcaatccc tgctcgtata tggcgtggcc cccttcgtga aacgaaccga aaagcgacgg
     2161 agtgaattcg tctggcggat acttggtggt aaccctgaca tcgccaaccc ctatacctat
     2221 tgtgaaggga tgaactgtct cgtccagccg gccggcccca aaatcgtaac cgatgaattt
     2281 gagggctttg tgactcaact tctcctgggt ctttctcgga aaccttccct ttagcagatc
     2341 ggtttcggga gcctttccct ccttcatgag ccttttcagg aaaggcacca gctccttctt
     2401 gaggggctct attatttcgc gcaacctctt cgcggtcata ccctcctcat aaccttccag
     2461 aagggcatcg taagggtttt cagcatagcc ataaagattg gccatctctc tggaaagact
     2521 cacgatttct tcgaggtaag gggcgaagcc ttcgaaatcg ttttccgccc gggccttttc
     2581 ccaggctttc tcactcctga cggtcagcac cgcgaatttc tgataaagcg ccggcggaat
     2641 cgccctggcc ctttcgaagg ctttggcaga gactctcact atggcctttt cctcatcgtt
     2701 gagagagttg tacgtctccg gctgtctaag attttccagg atcgacccca gttcgtccga
     2761 gactctcttt ttgaaggcca gtgtagatat ataaccgaga gcttctgcgc gcatttcgtt
     2821 gcccatgggt ggaagatacg tacgcatgtc ccaggccatg agagcgaccg cgctgttaag
     2881 aaaggaaagt tccgtgtacc gggagttcag ttcttcgatc agactcactt tcgcacctcc
     2941 aattttcgca attatatcat gctgttcact cgtgtttttt accctccaac atcgctcttc
     3001 gatctgggaa gatcgcaact attagaaagg aaacgaaata aggtatcgtc aacagcagtt
     3061 taggggagat ctgcgtcgat acctgaagga aaatagacag ttccgtcacg aaaccgagca
     3121 tcagggccga gaagccgatc agaaaaggtc tatctctccc cattattacc gccaccagcg
     3181 ctatccagcc tctgccaccg gacatattgg ccacgaagag tcccaacggc aaagaaagcg
     3241 acgccccggc cagtccggag aaaacacctg cgataataaa ggataccagt ctcactctgt
     3301 tgacattgac acccgagtag taggcactct cctcgtcgat gccacaggct atcagcttca
     3361 atccgaacga ggtttccctc agaacgaaca gcaagagcaa cggcgaagcg atcgccagat
     3421 aatcgaagaa gttcaggttg ttgagaaatc ccagaaagtc cagactcgaa acggccgggc
     3481 gtggcgcttc cgggaagacc acggttcctt tgctcctgaa ccatagatcg gcgagcatta
     3541 cggtgagacc tccggagaga aggttggtgg ctatcccggt gatgtacagg ttcgccctca
     3601 tgtacctgac catcacgaaa atcagcagag agactgcaat cgatgttgca atcgccgcga
     3661 gagtaccgac tacaacgctg gaagtcgccg atgatacgac aacccccgag aaggcggaaa
     3721 gaagtatcat gccttcgaga ccgatgttca
//
LOCUS       DAOOLY010000243         3738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29793_length_3738_cov_5.260114, whole genome shotgun sequence.
ACCESSION   DAOOLY010000243 DAOOLY010000000
VERSION     DAOOLY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29793_length_3738_cov_5.260114"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1489
                     /locus_tag="PLD15_10305"
     CDS             257..1489
                     /locus_tag="PLD15_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64344.1"
                     /translation="MKKLLVAVLLLSVSALLASGSVEIFSWWTGGGEEEGRAAIYEVF
                     KAKFPDVEIINATVAGGAGTNAKAVLKTRMLGGNPPDSFQVHGGMELIDTYVITSMME
                     PLTDILEGWGILDKFPKDIMTICSYEGEVYSIPVNVHRGNVMFINNEILKKAGVETVP
                     QDGPGFLQVCEKIKQAGFIPLALGDKDKWEAAHLFETVLLSALGPEGYNGLWNGTTSF
                     NDPGIGRAIVIFEELIKYVNEDHAALTWQDATKMVFDGKAAFNVMGDWAEGYLKTLGW
                     TPGVEFGWMAVPGTQGSFMVVTDTFGLPKNAPNRENALKWLEIVASVEGQDAFNPIKG
                     SIPARLDADKSLYDPYLTWSMNDFATNALCPSIAHGSAAPEGFITELNDAVNIFITTK
                     DRDGFLKAIRNAAEDYID"
     gene            1655..2473
                     /locus_tag="PLD15_10310"
     CDS             1655..2473
                     /locus_tag="PLD15_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64345.1"
                     /translation="MSNWNSFARLLKGEFEFVGLRNYLRLFEDPRFQTDLWNTFYFTL
                     FFIAGCLILGIILAVLIDRNLKGSAIFRNIYLFPMALSFVVTGAVWRWIFAPGILPGS
                     PQGMNLLLDLVGLDIFQWKWFTSTTSFLKFNVALIPVIIAAVWQLSGYTMAMYLAGLR
                     GISQDLIEAAEVDGATGWQIFWRIKFPILRPITLSAMIILGHISLKIFDLVYAMTGSG
                     PNNVTDVPAIYMFETTFRANKYATGSAIAMVMLIMVAVVIVPYLFSAFRKEKSI"
     gene            2470..3282
                     /locus_tag="PLD15_10315"
     CDS             2470..3282
                     /locus_tag="PLD15_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64346.1"
                     /translation="MIRKLVIYILLVVFALFFLMPVYVLLATSFKPLKEVGLVRMWFP
                     PNEFSFDGFVKAFNKLAPNLRNSFMLVIPATLLSAVIGSINGYVLSKLKFRGSDFLFA
                     LVLFGMFIPYQSVLFPLIRFLQDIGLYGSIWGLVLVHVVYGLPITTLIFRNYYSEVPT
                     ELIEAASIDGAGIFKTYLKVLFPISLPGFVVVVIWQFTNIWNEFLFAVTVTSDPTKQP
                     ITVALVNLAGSQVVEWNVQMAGALLAALPTLIVYILLGKYFLRGLLAGSVKG"
     gene            complement(3272..>3738)
                     /locus_tag="PLD15_10320"
     CDS             complement(3272..>3738)
                     /locus_tag="PLD15_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64347.1"
                     /translation="RNFRIPRQKWPHEPGVTLPGEEFGSFGDQPVWSRIVAFHEFCHP
                     LIDPLITAKPELVEALRTSPFSRGVLSAFMDRYPSWEDMLAEFLIYAMTYAYLFHEFD
                     RETAEIFHRTMEERSGFSGVRPMGEPLLRYLEERKNGEYTNLFDYLPVMFNL"
BASE COUNT          839 a          937 c         1043 g          919 t
ORIGIN      
        1 ctcttaccca caacacacca cgcacaacca acaacgtttc ttaagagaat tctcgccttc
       61 tcgtgagcga agcgagctct atcgttccgt cgtgtaaaga tcggtttgtt agtgaattgg
      121 ccatctgaca gagagagtag aaatgtattg agtaaatatt tccagttgta taataacaat
      181 gtaattatcc gttcggtctg gggggactgt attcgtaaag tacggcttaa aagccgaaaa
      241 cacagggggt gtttagatga aaaagctttt ggtagcggtt cttcttttat ccgttagcgc
      301 actcttagcg tccgggagtg tggagatttt cagctggtgg acgggcggtg gagaagagga
      361 gggccgcgcg gcgatatacg aggtcttcaa agcgaagttc cccgacgtcg agataataaa
      421 cgcaacggtc gccggtggag ccggaacgaa cgcgaaggcc gttttgaaga cgaggatgct
      481 gggtggcaac ccgccggatt ccttccaggt gcacggtgga atggagctta tagacaccta
      541 cgtcatcacg agcatgatgg aacctctgac ggacatcctc gaaggctggg ggatactcga
      601 caaattcccc aaagacatca tgacgatctg cagttacgag ggagaggttt actccatacc
      661 ggtgaacgtg cacaggggta atgtgatgtt catcaacaac gagattctga agaaagccgg
      721 tgtcgagacg gtaccgcagg acggaccggg gttcctgcaa gtctgtgaga agatcaagca
      781 ggccggcttc attcctctag ccctcggcga caaggacaag tgggaagcgg ctcatctctt
      841 cgaaaccgta ctgctttcgg ctctcggtcc tgaaggttac aacggtctat ggaacggcac
      901 gaccagcttc aacgacccgg ggatcggaag ggctatcgtt atcttcgaag agttgatcaa
      961 gtacgtcaac gaggaccacg ccgcgctcac gtggcaggat gcaacgaaga tggttttcga
     1021 cggtaaggcc gccttcaacg tcatgggcga ctgggccgaa ggttacctca agacgctagg
     1081 ctggactccc ggcgtcgaat tcggctggat ggccgtcccc ggaactcagg gctccttcat
     1141 ggtcgtaaca gataccttcg gtcttccgaa gaacgcgccc aacagggaaa acgccctgaa
     1201 gtggctcgaa atagtggctt cggtcgaggg acaggacgct ttcaacccga tcaaggggtc
     1261 gattccggcg aggctagatg ccgacaagtc gctttacgat ccttacctga cctggtcgat
     1321 gaacgacttc gccaccaacg cgctctgtcc gtcaatagct cacggttcgg cggctccaga
     1381 gggcttcata accgaattga acgacgctgt caatatcttc ataactacaa aagacaggga
     1441 cggcttcttg aaggcaataa gaaacgccgc ggaagactac atagactgat atgttcggcg
     1501 gggcctgcgt tgaagcgggc ccccttctct ggaggtgttg tagatcaagc ggaaaaccag
     1561 gcgtggaatt atttccttcc tcatactatc gccaacgttc gccgcgatag gtattttcgt
     1621 ttatttcttc ataggctgga cggccagaac gtcggtgtcg aactggaaca gctttgccag
     1681 gctattgaag ggagagttcg agttcgtggg tctgcgcaat tatctcaggc tcttcgaaga
     1741 tccgaggttt cagaccgatc tctggaacac tttctatttc actcttttct tcatcgccgg
     1801 ttgtctaata ctgggcataa ttctcgccgt tcttatagac aggaatctta aagggtcggc
     1861 tatattcaga aatatctatc tctttccaat ggcgctttcg ttcgtcgtca ccggggcggt
     1921 ctggcggtgg atattcgcgc cgggaattct accgggcagt ccccagggaa tgaatcttct
     1981 cttggatctg gtgggactcg atatatttca gtggaagtgg ttcacgagta cgacgagttt
     2041 tttgaagttc aacgtggctc tgattccggt cataatcgcc gccgtctggc agctttcggg
     2101 ctatacgatg gcgatgtatc tggccggact gcgcgggata tcgcaggatc tgatcgaagc
     2161 ggccgaggtc gatggcgcga ccggctggca gatcttctgg aggataaagt ttccgatact
     2221 gaggcccata acgttgagcg ccatgataat tctgggacat atctcgttga agatcttcga
     2281 cctcgtttac gcgatgaccg gcagcggccc aaacaacgtc acggatgtgc cggccatcta
     2341 tatgttcgag actactttca gggcaaacaa gtacgccaca ggctcggcga tcgccatggt
     2401 aatgctgatc atggtcgccg tggtaatagt gccgtacctc ttctcggcgt tcagaaagga
     2461 gaaaagtata tgatcaggaa gcttgtgatc tacatacttc tggttgtttt cgccctgttc
     2521 tttctgatgc cggtttacgt tctcctggcc acgagtttca agcccctgaa ggaggtcgga
     2581 ctggtgagaa tgtggtttcc accgaacgag ttctccttcg atggtttcgt caaggccttc
     2641 aacaaactgg ctcccaacct gagaaactct ttcatgctgg tgataccggc cactctgtta
     2701 tcggccgtga tcggttcgat caacgggtac gttttatcca agttgaagtt caggggatcg
     2761 gactttctct tcgccctcgt tctcttcggg atgttcatcc cctaccagag cgtgctcttc
     2821 ccgctgatac gctttttgca ggatataggt ctttacggtt cgatctgggg gctggttctg
     2881 gtgcatgtgg tttacgggtt gcctataacg actttgatct tcaggaatta ttacagcgag
     2941 gtgcctaccg agttgataga agcggccagc atagacgggg ccggtatctt caagacgtat
     3001 ctcaaggttc tcttccccat atcgctgccg ggcttcgtgg tcgtggtaat ctggcagttc
     3061 acgaacatat ggaatgaatt tctcttcgcc gtcaccgtca ctagcgaccc gacgaagcaa
     3121 ccgatcaccg tggcgctggt aaacctggcc ggaagccagg tggtggagtg gaacgtccag
     3181 atggccggcg ccctgctcgc ggctcttcca acgttgatcg tctatatact tctgggcaag
     3241 tacttcctga gggggctcct cgccggctct gtcaaaggtt gaacatcacc ggaaggtaat
     3301 cgaaaaggtt cgtgtattcg ccattctttc tttcctccaa ataacggaga agcggctctc
     3361 ccataggtct tacgcccgag aagccggacc tctcttccat ggttctgtga aaaatttcgg
     3421 ccgtctcccg atcgaactcg tggaaaagat aggcgtaggt cattgcgtat attagaaatt
     3481 cggccagcat gtcctcccag gaagggtacc tgtccatgaa ggcggaaagc acgccacgtg
     3541 aaaagggcga agttctcaac gcttccacca gttcgggctt tgccgttatg agcgggtcta
     3601 tgagtgggtg gcagaactcg tggaaggcga ctatccttga ccagactggc tggtcgccga
     3661 aacttccgaa ttcctcgcca ggcagggtga ctcccggttc gtggggccat ttctggcgag
     3721 gaattcggaa gtttcggc
//
LOCUS       DAOOLY010000244         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29838_length_3733_cov_4.875476, whole genome shotgun sequence.
ACCESSION   DAOOLY010000244 DAOOLY010000000
VERSION     DAOOLY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29838_length_3733_cov_4.875476"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLD15_10325"
     CDS             <1..1011
                     /locus_tag="PLD15_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB64348.1"
                     /translation="RMKFDSVVILPEEMSAERFEIIREYGARVIATPGCESNVKEIYD
                     KAKELKSADPDKVRILNQFEEFGNYRFHYYVTGNTILELMEQLKIGNGRVSAIVSTVG
                     SAGTIACGDRVKEVFPEVKIVAGEPVQCPTLSLNGYGGHDIQGIGDKHVTWIHNVLNN
                     DAIVALDDIECKKVLQTMTDEAGKSLLKEFLSDKDVEKMAGIFGISGVANVLGAIKIA
                     KHYRLTSRDNIVTIATDNMDRYHSVMENMTRTYGKLDRGEAKQRFESIVFGAGTDWIF
                     EGTMENRLRWHNLKYYTWVEQQGKTVEELNRTMDQGFWKDEAQKTGEIDGLLIDHRRK
                     NG"
     gene            complement(1028..2872)
                     /locus_tag="PLD15_10330"
     CDS             complement(1028..2872)
                     /locus_tag="PLD15_10330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPB64349.1"
                     /translation="MKKTYDWAVDTVSLLASMSLIKVFFFYIYTAQTLKLSNIIGTIV
                     WYFFLLITFRVLFKGRFFLLYCIVSCVLFIDFLYFQYFGFLPSVKELAHVHHIGGVKS
                     SIIYVMDPLSFLFIIDLVPVGLYLRRKLSRAGETVEKSSGRAQWLAMFSILFLALVLP
                     LISESLQPYFVFNRYGLFAYHLHDMAGLALGKIKAPIVDESVTYSGYINRVAEKGKYF
                     ALARNRNIILVQLESFQDFLVGFSYNGQEITPNLNRLISNNSIYFNEIYQQVGAGNTS
                     DAEFVINNSMHGLGQISVYESRDQNSFYSLPALLNSKGFYTIAFHGNKGSFWNREKVY
                     PSLGFDDFVSLEEMENDEIIGFGLSDLSFFRQVVTMIKSLPSPFFAFLVTLTSHNPYD
                     IPGELHGIKLLPEHEGTLFGNYLQSVHYTDKALGVFISDLKHVGLYENSIVAIYGDHS
                     GLYPFNKENRDIMTGVLGKTYDLVQAMNIPLVFHIPGSRLKVVSSIAGGQIDFFPTLL
                     NLIGVVEKNGILFGRDLNNSSGGFVSLTHYVPEGSFIDDGRIFIMSGDGILENSIAID
                     RTTGETIPSFSCLDGYKDSIQQIEASKYLILNDSISQLLGQATSTNTN"
     gene            3011..>3733
                     /locus_tag="PLD15_10335"
     CDS             3011..>3733
                     /locus_tag="PLD15_10335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB64350.1"
                     /translation="MVEHGATDEKDLSCGVKRNDLFEILKKNRDRIRQLHNVALQLSK
                     AENEERIYDLIIEASRKILKFDTYSLDIVEGENLVVKRISECVTDSDTQVYSKQDGIA
                     GRTLREGKTIALGSLAGNCEAKPISSDYKSALSIPVGIYGVFQTASNVEDAYREEDID
                     LAELLIAHAAEAISRVRARKEIFRLTYHDCLTGALSRYGLSEFAMMEIEKARKCDSPL
                     SMIMIDIDNFKAINDSMGHVYGD"
BASE COUNT         1101 a          837 c          952 g          843 t
ORIGIN      
        1 cggatgaagt tcgactcggt ggtgattctg cccgaggaga tgtcggccga aagattcgaa
       61 atcataaggg aatacggggc ccgcgtgata gccacgccgg ggtgcgaatc gaacgtcaag
      121 gagatatacg ataaagctaa agagcttaag tctgccgacc ctgacaaggt taggatactc
      181 aaccagttcg aagagttcgg caattacaga ttccactatt atgtgaccgg aaacaccatc
      241 ctcgagctaa tggaacagct gaaaatcggt aacggaagag tatcagcgat cgtctcgacc
      301 gtcggctcgg caggcacgat cgcctgcggc gaccgtgtga aagaggtctt cccggaggtt
      361 aaaatagtcg ccggcgaacc ggttcaatgc ccgacgcttt ctctgaacgg ctacggtggt
      421 cacgacatac agggaatcgg cgacaagcac gtgacctgga tccacaacgt actcaataac
      481 gacgccatcg tggcgctcga tgatatcgaa tgcaagaaag tcctccagac gatgaccgat
      541 gaggctggaa aatctctctt gaaggaattt ttgagcgata aagacgtgga gaagatggcc
      601 gggatattcg gaatctcggg cgttgcgaat gtgttggggg cgataaagat agccaagcac
      661 tacaggctga cttccagaga caacatagtg acgatagcca ccgacaacat ggatcgctac
      721 cactctgtca tggaaaacat gaccagaaca tacggaaagc tcgaccgcgg cgaggccaaa
      781 cagaggttcg aatccatcgt gttcggagcc gggaccgact ggatcttcga gggaacgatg
      841 gaaaacaggc tgcgctggca caacctaaag tactacacct gggtcgaaca acagggaaag
      901 acggtggaag agctaaacag aaccatggac caggggttct ggaaggacga ggctcaaaaa
      961 accggagaga tcgacggcct gctgatcgat cacagaagga agaacggctg acggtcattt
     1021 tccggtgtca gttggtgttt gtggaagtgg cctgacctaa aagctgggag atagagtcgt
     1081 tcaatatcaa gtattttgaa gcctctatct gctgaataga atccttataa ccgtccagac
     1141 agctgaacga ggggattgtt tctccagttg tcctgtcgat ggctatgctg ttttcaagaa
     1201 tcccgtcgcc ggacattatg aatatcctcc cgtcatctat gaaggatccc tctggcacgt
     1261 aatgtgtcag agaaacgaag ccgcccgacg aattgttcag gtctctaccg aacagtattc
     1321 cgtttttctc gacaacgcca atcagattca ggagagtggg gaaaaaatct atctggcctc
     1381 cggctatgga gctaactacc tttagcctgg agccaggtat atgaaagacc agtggtatgt
     1441 tcatggcctg aaccaggtcg taagtcttgc cgaggacccc ggtcataata tctctgttct
     1501 ctttgttgaa aggataaaga ccggaatggt ctccatatat agcaacgata gagttctcgt
     1561 agaggccgac atgtttcaga tcggatatga aaactcccag agctttatcc gtgtaatgga
     1621 ccgactggag atagttgccg aaaagagttc cttcgtgttc ggggagtagc tttattccgt
     1681 gaagctcgcc aggaatatcg tacgggttgt gactggtgag tgtgacaagg aaggcgaaaa
     1741 aaggagaggg cagagatttt atcatcgtca ctacctgcct gaaaaaggag agatcgctca
     1801 aaccaaagcc gatgatttcg tcgttttcca tctcttcgag actcacaaag tcgtcgaatc
     1861 ccagcgaggg gtaaactttc tcccggttcc agaaggagcc tttgtttccg tgaaaggcga
     1921 tcgtgtagaa gcctttcgaa ttcaagaggg ccggaaggct gtaaaaactg ttctgatcgc
     1981 gtgattcata gacagagatc tgccccagac catgcataga attgtttata acgaattcag
     2041 catccgatgt gttgcccgct ccgacctgct gatagatttc gttgaaatag atggaattgt
     2101 ttgaaatcaa cctgttgaga tttggggtaa tttcctgacc gttgtaggag aaacctacaa
     2161 gaaagtcctg gaaggattcc agctgtacca gaatgatatt cctgttcctg gccaaggcaa
     2221 aatactttcc cttctctgca actctgttta tataaccact ataggtaaca ctctcatcga
     2281 caatgggggc tttgatctta cccagtgcca gtccggccat atcgtgcaga tggtaagcga
     2341 agaggccgta gcgattgaaa acaaagtaag gctgtagtga ttcggagatg agcggcagca
     2401 cgagagccaa gaataaaatc gaaaacatcg ccagccactg cgctctcccg ctagattttt
     2461 caacggtctc gccagctctc gagagcttcc gcctcaggta taatcccaca ggaacaagat
     2521 cgattatgaa gagaaaactc agcggatcca tcacatagat tatgctgctc ttcacaccgc
     2581 ctatgtgatg aacatgggcc agttccttca ccgagggcag gaatccgaag tactggaagt
     2641 aaagaaagtc tatgaagagc acacaggata ctatacagta aagcaggaag aacctgcctt
     2701 tgaatagaac ccgaaaagtt ataagcagaa agaaatacca gacgatcgtt ccgattatgt
     2761 tggagagctt caaggtctga gccgtataga tgtagaaaaa gaaaaccttt atcagactca
     2821 ttgaagcaag caaagagaca gtatcgactg cccagtcata agtttttttc aagtgattca
     2881 cacccgatcg attttttcca gcttacctat tgagaaatta tacatactct atcgattcat
     2941 atcaaccctt gaatcataga tggcatagat ttgcaagaca ccaattaacc atacgaaagg
     3001 tgtgtgaatt atggtcgaac acggcgccac tgacgaaaaa gacctcagct gtggtgttaa
     3061 aagaaacgat ctcttcgaaa ttctgaagaa aaaccgtgac aggatacgtc agttgcacaa
     3121 tgtggctctg caactttcga aggcagagaa cgaagagaga atctacgact tgataatcga
     3181 agcctccaga aagatcctta agttcgatac gtatagcctc gatatagtgg aaggcgagaa
     3241 tcttgtagtg aaaaggattt ccgagtgtgt taccgatagc gacacccagg tttacagcaa
     3301 acaggacgga atagccggaa gaactctgag agaaggtaaa acaatagcct taggaagcct
     3361 agcaggtaat tgcgaagcca aacctattag cagcgactat aaatcggctt tgagcatccc
     3421 cgttggtatt tacggagtct tccagacggc ttcgaacgtt gaagatgcct atcgcgaaga
     3481 ggatatagat ctcgccgaac tgctcatcgc acacgctgca gaagctataa gtcgtgtcag
     3541 ggcgaggaaa gaaatattca ggcttaccta tcacgactgc ctgaccggtg cgctcagcag
     3601 atatggactc tcggaattcg caatgatgga aatcgaaaag gcaaggaaat gtgattcccc
     3661 cctatcgatg atcatgatag acatcgacaa tttcaaggcg ataaacgatt cgatgggtca
     3721 cgtgtacggc gac
//
LOCUS       DAOOLY010000245         3726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29914_length_3726_cov_5.108145, whole genome shotgun sequence.
ACCESSION   DAOOLY010000245 DAOOLY010000000
VERSION     DAOOLY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29914_length_3726_cov_5.108145"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLD15_10340"
     CDS             <1..338
                     /locus_tag="PLD15_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPB64351.1"
                     /translation="LDNHSIDGVVVARGAIGNPWIFKQARDLMELGTYEEFGLSERLE
                     HFLNHIERLADVVGENQAVRDLRKSFAGYTRNIRYASNMRKEYMKCVSLEEVRELFRA
                     FVPDFQVFK"
     gene            434..2038
                     /locus_tag="PLD15_10345"
     CDS             434..2038
                     /locus_tag="PLD15_10345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB64352.1"
                     /translation="MKKAVLLVLLVFFSITLQAVEYAVKVTRNGEALPEEFWISSEEL
                     VQAFYSNVSDAAGQGIVFDPYFTNMNIPSEHNMKLIIISYLVDEKLVDYYILENGLLP
                     SEDEVASETAAMLSMYTSDPYTVAQIESIYGSIDAFAELIEEYVAYSLKMDHFLNKVI
                     PTDEQSLLTYFTENIETIRSFYETVRAKHILVIEEATALSLKNKIKNGEITFADAAAQ
                     YSEDSNSALNGGELDYLSRGDTVAEFEEAIFTAPVGELYGPVKTLSGYHLIIVEERIE
                     IDSLSDLQASEDTYSDFVERYRDEAYVEWIEAYIDTNKFDFEILDSELLFYKSFIDAS
                     ADRESSLEMTAGLTARLFGDGVVENPAPVEYAVFIELSQMLGLTNDPRYEMAITRLYE
                     TGEKKGRILQMMYELKGDDAVVVGDYYNEILQDLERTFSDSAYFYAKQSSYGMDFVDY
                     VLGTIEEIETALGVFLERELSVDTRVYLLSILLRNNSLALDINDDEAWVDKRLNLRLE
                     YLESLMGIAPSKEVEEDIEKIRKMLE"
     gene            complement(2192..2815)
                     /locus_tag="PLD15_10350"
     CDS             complement(2192..2815)
                     /locus_tag="PLD15_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64353.1"
                     /translation="MRRMLIILTAMLITGVVVFGAIGADDPDPYENVNSEYSPTEARD
                     PFIEILTGNKTGVYIGDWLNIEGESFAFSLPAGFSVKFFPDTYEIADIIFYEAVVSKI
                     IAGYVYESEPVIDTIGKIVGEYLYNLGDYSVTESLSIFVADGSINEVYSLNFSGMPCW
                     FVLNSQSQTPGEFGPGRFFVFLGLPDEIEIEIWASLYSGIVQSVNLF"
     gene            complement(2895..3497)
                     /locus_tag="PLD15_10355"
     CDS             complement(2895..3497)
                     /locus_tag="PLD15_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64354.1"
                     /translation="MRRITILFFIAFLACSALSSALDRNDSAAASDPFIELMKGIVNG
                     KISIVTDDWIDVKGRYMALYIPPEFAVSFTFDETSETADLFLEGISVGKILVGEFYET
                     SSYTGNIFDEIVEEYRMIFNADNYEIADSLSIQRDKDSMNLYLINFIGQRCWIALYSE
                     SPDIDVFGYGKYFVFVGLPEEERLDQLANFFGGILTTLSF"
BASE COUNT         1047 a          879 c          859 g          941 t
ORIGIN      
        1 ctctcgacaa tcactcgata gacggcgttg tagttgcaag aggggcgata ggcaacccct
       61 ggatcttcaa acaggccaga gatttgatgg agctcggcac ttacgaggag ttcggtttga
      121 gcgagagact ggaacatttt ctgaaccaca tcgaaaggct ggccgacgta gtcggcgaga
      181 atcaggccgt tagagacctg cgcaagtcct tcgccggtta caccagaaac atcaggtacg
      241 cctcgaatat gagaaaggaa tacatgaaat gcgtctctct cgaggaggtc cgggagcttt
      301 tcagagcctt tgtaccggat ttccaggtat tcaaatgatc cgttgttttt ttcgtttccc
      361 gaacccgttt tgacaattcc cgtatatcaa agtgttatca ttgttgcatc tactatatta
      421 gggggcacag gctatgaaaa aagctgttct actggtgcta ctggtgtttt tctccatcac
      481 tcttcaggct gttgaatatg cagtaaaagt taccagaaat ggcgaggctc ttcccgagga
      541 gttctggata tcttcagagg aactagttca ggctttctat tcgaacgttt cagacgctgc
      601 cggccaggga attgtgttcg acccgtactt cactaatatg aacattccaa gcgagcacaa
      661 catgaaactg ataatcatct cctatttagt tgacgagaag cttgtcgact attacatact
      721 ggagaacggg ctcctcccat cagaggatga agtcgccagc gagacggcag ccatgctttc
      781 gatgtacaca tccgatcctt acacggttgc acagatagaa tctatttacg gctcgataga
      841 tgcctttgcc gagttaatcg aggaatacgt cgcctattca ttaaagatgg atcacttcct
      901 gaacaaagtg atcccgaccg atgagcaatc gcttctgact tactttacgg aaaacatcga
      961 aaccattcga agcttttacg agacggtgag ggcaaagcac atattggtca tcgaagaggc
     1021 cacggcactt tctttaaaaa acaaaataaa gaatggagag ataaccttcg cggatgcggc
     1081 cgcgcagtat tcggaggatt ccaattccgc cctgaacggc ggagagctgg attatcttag
     1141 tagaggcgac accgtcgccg agttcgagga agccatcttc acggcacctg tgggcgagct
     1201 ctacggcccc gtgaagactt tatcgggata tcacctgata atagtcgaag aaaggataga
     1261 gatcgattca ctttccgacc tacaagccag cgaggataca tacagcgatt ttgtcgagag
     1321 atacagggac gaggcgtacg tagagtggat cgaagcctat atagatacca ataaattcga
     1381 tttcgagata ctcgacagcg aactcctatt ctacaaaagc ttcatagacg catccgccga
     1441 cagagaaagc tcgctggaaa tgactgccgg tctaacggcc agacttttcg gcgatggtgt
     1501 ggtcgaaaac cctgcgccgg ttgagtacgc cgtttttatc gagctttccc agatgctcgg
     1561 tttgacgaac gatccgcgct atgaaatggc gataaccagg ctgtacgaaa caggtgagaa
     1621 aaagggccgg atacttcaga tgatgtacga actgaaaggc gacgacgccg ttgttgtcgg
     1681 cgattactac aacgaaattc ttcaggatct cgaacgtacg ttcagcgatt cggcatattt
     1741 ttacgccaag cagagtagtt acggcatgga ctttgtggat tatgtgctgg gcactatcga
     1801 agagatagag acggccctgg gagttttcct ggaaagagaa ctctcggttg ataccagagt
     1861 gtatctcctg agcatattgt taagaaacaa ctcgctcgcc ctggatataa acgacgacga
     1921 ggcgtgggtc gacaaaagac tgaacctgag gctggaatac ctagaatcgc tcatggggat
     1981 cgccccttcg aaggaagtcg aagaagacat cgaaaagata cgcaagatgc tcgagtgatt
     2041 cacttcaatc tttagaatca accgatcagt tgaacatttc atccgttgcc gaccggagac
     2101 agagatctcc ggtcgtttca tctagtatca taaaaacgct ttcgatcggc catcagtttg
     2161 tctctataac tggtgacaca tttctgattg cctagaataa attcaccgat tgcactattc
     2221 cggaataaag ggaagcccaa atttctattt ctatttcgtc cggcagaccg aggaaaacaa
     2281 agaacctgcc agggccgaac tctccaggcg tttgcgactg agagttgagt acgaaccagc
     2341 acggcattcc agagaagttg agtgagtaaa cctcattgat cgagccatct gcaacgaata
     2401 ttgaaaggct ttccgttact gaatagtcac ctaaattata taagtactca cccacaattt
     2461 tgccgatagt atctatgaca ggttcgcttt cgtaaacgta acccgctatt atcttgctaa
     2521 caactgcctc atagaaaata atgtcggcta tctcgtaggt gtccggaaag aatttgaccg
     2581 aaaaacctgc tggcaaagag aatgcgaagc tttcaccttc gatattgagc caatcaccta
     2641 tatacacacc cgtcttattg cctgtcaaga tctctataaa agggtctcgc gcttcagttg
     2701 gtgagtattc gctgttgacg ttttcgtatg ggtccgggtc atccgcgccg atcgctccga
     2761 aaacgactac gcccgttatc aacatcgccg taagaattat caacattctt ctcatagtac
     2821 accccctgta aaagataaat ccgcatttaa aaaacccata cgaattctat gacttattat
     2881 gcaagtctgg aagttcaaaa gctgagcgtc gttaaaattc ctccaaagaa gtttgccagc
     2941 tgatccagtc tctcttcttc aggcagaccc acaaagacga aatacttacc gtatccgaag
     3001 acatcgatat ctggagattc cgaataaaga gcgatccagc agcgctgccc gatgaaattt
     3061 atcaaataaa gattcataga atctttgtcc ctctgaatcg aaagactgtc ggctatttcg
     3121 tagttgtctg cgttgaaaat catgcgatac tcctcgacta tttcgtcgaa aatattcccg
     3181 gtgtaagaag aagtctcata aaattctccg accagtatct tgccaacgct gatgccttct
     3241 aaaaataaat ctgcggtttc cgaggtctca tcgaaagtga aactcacggc gaattccggc
     3301 gggatataca acgccatgta tctgccctta acgtctatcc agtcgtcggt aacgatagag
     3361 atcttgccgt tgacaatgcc tttcataagt tcaatgaaag gatcgctcgc cgctgctgaa
     3421 tcgttccggt caagagctga ggacagtgcc gaacacgcta aaaacgcaat aaaaaacagt
     3481 atcgtaatcc ttctcacggg tacacctccg gtaaaatcac agaattttcc taagaagatt
     3541 atacaatttc taaattacgg aaaagacccg gcattgccgg gtctttttcg aacgaagttg
     3601 cttgcaagta ttacttcagt tccaccgtcg tggtgatgac tccgcctctg gtagaagaga
     3661 actttatcgt ggcggttcca gtaccggtta cggtccagga aacggtcttc tctctgtccc
     3721 acttcg
//
LOCUS       DAOOLY010000246         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_29952_length_3723_cov_4.944384, whole genome shotgun sequence.
ACCESSION   DAOOLY010000246 DAOOLY010000000
VERSION     DAOOLY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29952_length_3723_cov_4.944384"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLD15_10360"
     CDS             <1..286
                     /locus_tag="PLD15_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64355.1"
                     /translation="GFSRSSNMKPSLYVGFMLLDLENPRRVLKRSQEPILEPEEEWEI
                     FGGVPNVVFSDAMVEHEGKYYIYYGAADNHIALATIEKEKVFDWIRGMGG"
     gene            291..1238
                     /locus_tag="PLD15_10365"
     CDS             291..1238
                     /locus_tag="PLD15_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPB64356.1"
                     /translation="MKVLLTLVAILLCAAALGVSLRFDDLYRVERYSDLPLLMPQGIG
                     WESKAVFNPTAIVVDSLVHMFYRAEDWAGAGRWNGTSRIGKATSIDGFDFTREASPAI
                     EPTEIYELPGGCEDPRIVRVDDLYIMTYTGYDGGKARLCIATSSDLATWEKLGPVFAN
                     DTWSKSGAIVPAKINGNYYMYFGDSSIKLAYSKDLKNWTVYPRPVLEPRQDRFDSRLV
                     EPGPAPLITEEGILLIYNSADFSTIYRPGAALFAVDNPRNLLKRTNVPLMEPELSWEK
                     LGQVPNVIFIEGSVMVGNKLILYYGAADTYIGTAVVELE"
     gene            complement(1410..2870)
                     /locus_tag="PLD15_10370"
     CDS             complement(1410..2870)
                     /locus_tag="PLD15_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB64357.1"
                     /translation="MKRFGLSSQIWLLFGLVMLLLCVILAVVFRWSIRDFFTTEAYMT
                     IESAQEMKLLELQGGLVPGSNIYMGDLKNIRDVGHIIVLNKEYGLLEEYLEGWRLKIT
                     GPNVREDLRTGSGQSFKLEISSIVIWGTISPDILPLLGEIRENAELQKAKSVRYETDI
                     GNRKLFYVIRSEELSDRNITFISYMWDTYKNTLSSTLFSRLFLVIAVVVILSLIIAIL
                     FSRYLTRPLKKLSEDVKAIAARNWDKPISINRKDEIGELADSMEMMRASLSEQDREQQ
                     AMLQFISHELKTPVMVIRSYAQAIKDGVYPGGDLDSAIDVIDREIQRMDMRVKDLLFI
                     TRLEYLSRHNLQLETIDLSELIESVAGRFYYQRADVEWDLRLEKIPASVNEEQFSIAI
                     ENILTNQLRYARQKIEIELKTIQKDSKIALTFKNDGEAIEEKRFQQLFKPFNKGARGE
                     HGLGLNIVKRIIELHNGRVWIENEEDGVSTHIELPV"
     gene            complement(2867..3541)
                     /locus_tag="PLD15_10375"
     CDS             complement(2867..3541)
                     /locus_tag="PLD15_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB64358.1"
                     /translation="MSIRINLVEDEKSLNDVLTSYLQREGWEVKSFYNGGDAFEEIDD
                     RPDLWILDIMLPGVDGFELLREIRSRNSTMPVIFISARDKDIDRVIGLEMGSDDYMPK
                     PFLPRELVIRVKKILERVQTTGELVSIPPYTIDESKRKVTVDSSPGTPVEIELTSMEF
                     DLLMLFVHGRGKPFSRDQILDKIWGYDHYGSDRAVDDLVRRLRRKMPDFKIETIYGFG
                     YKGVDV"
BASE COUNT          854 a          960 c          933 g          976 t
ORIGIN      
        1 cggattttcc agatcgagca acatgaagcc gagtctgtac gtcggcttca tgttgctcga
       61 tctggaaaat ccgcgcaggg ttctgaagcg ttcgcaagaa ccgatcctcg aaccggaaga
      121 ggaatgggag atcttcggcg gcgttcctaa cgtggtcttt tccgacgcca tggtcgagca
      181 cgaaggcaaa tactatatct actacggcgc tgccgacaac cacatagcgc tggccacgat
      241 agaaaaagag aaagtgttcg actggatcag agggatgggg gggtgatgtt ttgaaggttt
      301 tgctgactct tgtggcaata ctcctgtgcg cggctgcact tggggtctcg ctccggttcg
      361 acgacctgta cagagtcgaa agatactcgg atctgcccct tctcatgcct caaggtatag
      421 gctgggagag caaggccgtc ttcaacccga cggcgatagt tgtcgattca ctcgtgcata
      481 tgttctacag agcggaggat tgggccggtg ccggcaggtg gaacgggaca tcgcgtatag
      541 gcaaggccac gtcgatcgac ggcttcgatt tcaccagaga ggcctcaccg gcgatcgaac
      601 cgaccgaaat ctacgaactg cccggcggct gtgaagaccc gagaatagtc agggtagatg
      661 atctgtatat catgacctac acgggctacg acggaggcaa ggcaaggctc tgcattgcga
      721 cctctagcga cctcgcgacc tgggagaaac tggggccggt cttcgccaac gatacgtggt
      781 cgaagtccgg ggcgatcgta cccgcgaaga taaacggaaa ctattatatg tatttcggcg
      841 actcgtctat aaaactggcc tattcgaagg atctgaaaaa ctggaccgtc tatcccaggc
      901 ccgtgctgga gccgagacag gacaggttcg acagcaggct ggtagaaccg ggaccggcgc
      961 cgctaataac tgaggagggt atcctgctca tctacaacag cgccgacttc tccactatat
     1021 accgtccggg ggcggctctc ttcgccgtgg acaacccgag aaacctgctg aagagaacca
     1081 atgtgccgct gatggaaccg gaactctcct gggaaaagct gggacaggtg ccgaacgtta
     1141 tcttcataga gggctcggtg atggtaggaa ataaactgat cctctattac ggcgccgccg
     1201 acacatatat aggaacggcg gtggtagaac tcgaatgaga gctgaagagc tggcggagcc
     1261 gtccatagtc cttcgttaga gcttggaccc ttccttggga agagcgagat ctcgtttaag
     1321 atcacaacgg aatgacggtt atgaaggagc tctcgaatgc gctctttctc tcgtgtttta
     1381 ccagcaacct ttcttaggat gccggacctt taaacgggca gttctatatg tgtcgagact
     1441 ccgtcttctt cattttcgat ccataccctg ccgttgtgga gttcgattat tctcttcact
     1501 atatttagtc caagcccgtg ttcgcctcta gcgcctttgt taaagggttt gaagagctgc
     1561 tggaatcttt tttcttctat ggcctctccg tcgttcttga aagtgagggc tatttttgaa
     1621 tctttctgga ttgtcttgag ctcgatctcg atcttctgcc tggcgtaacg cagctggttg
     1681 gtgaggatgt tctcgatggc tatcgagaac tgttcttcgt tgaccgacgc cggaatcttc
     1741 tccagcctca gatcccattc cacatcggct ctctggtagt agaaccttcc ggcgacactc
     1801 tctatgagct cggaaagatc tatcgtctcg agttgcaggt tgtgtctgga cagatattca
     1861 aggcgcgtga tgaaaagcag atctttcacc ctcatatcca ttctttgtat ttccctgtct
     1921 ataacatcta tggcgctatc gaggtctcca ccaggataga cgccgtcctt tatggcctgg
     1981 gcatagcttc tgataaccat aaccggtgtt tttagctcgt gagagatgaa ctgaagcatc
     2041 gcctgctgtt ctctgtcctg ttcgctaagg ctggctctca tcatctccat cgaatcggcc
     2101 agttcgccga tctcgtcctt gcggtttatc gagatgggtt tgtcccagtt tctcgcggct
     2161 atggctttaa catcttcgga gagttttttc aacggtcgcg tcaggtatct cgaaaagagt
     2221 atggcgatta tcaggcttag aatcacaacg actgcaataa caagaaatag acgcgagaaa
     2281 agcgtgctgg agagtgtgtt cttgtaggta tcccacatat aggagatgaa agtgatattt
     2341 ctgtcggaaa gctcttccga tcttatgaca tagaagagtt tcctgttccc gatatcggtt
     2401 tcataacgaa cggattttgc cttctgtagc tcggcgtttt ctcttatctc accaagaagg
     2461 ggaagtatat ccggggagat ggtaccccat atgactatac tcgagatctc cagtttgaag
     2521 gactggccgg agcccgttct taagtcctcc cttacgttgg gaccggttat tttcaacctc
     2581 cagccctcca ggtactcctc gagaagaccg tactctttat tcaggacgat tatgtgaccg
     2641 acgtccctaa tattcttcag gtcgcccatg taaatattgc ttccgggaac cagcccaccc
     2701 tgtagttcaa gcaacttcat ttcctgggcc gattctatcg tcatgtaagc ctctgtcgtg
     2761 aagaaatccc ttatggacca cctgaagacc acggcgagta tcacacatag aagtagcatc
     2821 acaaggccga agagaagcca tatctggctg gaaagaccga acctcttcat acatctacgc
     2881 ctttgtagcc gaagccgtag atggtctcga tcttgaaatc gggcatcttt cgcctcaacc
     2941 tcctgaccag atcgtccacc gccctgtcgc ttccgtagtg atcgtaaccc cagatcttat
     3001 ccagtatctg atcgcgggaa aagggctttc cccttccgtg aacgaatagc atgagaagat
     3061 cgaactccat cgacgtaagt tcgatctcga cgggggtccc tggtgaagaa tctacggtga
     3121 cctttcgctt gctctcatct atcgtgtaag gaggtatcga caccagctct ccggtagtct
     3181 gtacacgttc gagtatcttt ttgaccctta tgaccagttc acgcggcaga aagggcttgg
     3241 gcatatagtc gtcacttccc atttcaaggc ctataactct gtctatatct ttatctctgg
     3301 ccgagatgaa gatcaccggc atcgtggaat tcctgctgcg gatctctctc aacagctcga
     3361 agccgtcaac gcccggaagc atgatatcca gtatccacag atccggtctg tcgtcgatct
     3421 cctcgaacgc gtcacccccg ttgtaaaagg atttaacttc ccagccctct ctctgaaggt
     3481 aggaagtcaa cacgtcgttc aaactttttt cgtcttccac aaggtttatc cttatggaca
     3541 tggttaccac ctctgtatcg acagaaaatc gcacttcaag tatattctat cttttcgaat
     3601 ctaaaaaacc ggaccgcttg acttgttttt tgctacctat acggtccggt gttaccgctc
     3661 tcacgatcgg ccttttcagg ggtagatagg gattctgggt gatgacttct agactcgtat
     3721 tct
//
LOCUS       DAOOLY010000247         3718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_30027_length_3718_cov_3.790063, whole genome shotgun sequence.
ACCESSION   DAOOLY010000247 DAOOLY010000000
VERSION     DAOOLY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30027_length_3718_cov_3.790063"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLD15_10380"
     CDS             <1..711
                     /locus_tag="PLD15_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPB64359.1"
                     /translation="EAFKALKPGVEISIEGAGSSTGIKALFNGQTDTANSSRWIKPSE
                     IEQMNRDGKYFVPYIVAYDGIAIIVNRSLAIDNITLQQLYDIYSGKVTTWNQIDSSLP
                     RVRIVPFSRDNASGTFEYFIEHVMKGDKLAPQVQQLASTRAEVEQVVQNNYAIGYIGM
                     GYITGEVKALTVEGVEPTVANVNAGTYPVSRPLFMFVNATNGLPTGVVADFLRYALSP
                     EGQAAVLTVGYVNAYGTK"
     gene            728..1603
                     /gene="pstC"
                     /locus_tag="PLD15_10385"
     CDS             728..1603
                     /gene="pstC"
                     /locus_tag="PLD15_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPB64360.1"
                     /translation="MRRKRVELSAKVLVTFAALLTSIIVFGIFYFLISDGVRVFGRVS
                     PKDFLFSARWYPTYEDAEFGILALLAGTLSVTGLTLVIAIPLGFVCAIFLAEFSGRKS
                     HDAIKYIFELMAGIPSVVLGSFGLKYVSRWLMEHFPLHVWTGLNVMNASLMLSILAMP
                     YFVTLVEDALRAVPRDQKEASLALGANMTGTIFRILLPQARSGILNAIILGTNRVIGE
                     TMVVLMVAGGATMVPSTIFDPVRPLTSAIAGEMGEVELGSTHYFALFMIGVVLLAIST
                     VFTLIAMKLKRSKGK"
     gene            1600..2436
                     /gene="pstA"
                     /locus_tag="PLD15_10390"
     CDS             1600..2436
                     /gene="pstA"
                     /locus_tag="PLD15_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPB64361.1"
                     /translation="MKIDAIVKITFAVVSYTLIGLIVALIGFLIVNGLDNIDLEFLTA
                     FPRNSMTGGGIWPALVGTVYFVAVALLFSIPVGILAAVYLSEYSKDTAFKRLILTANN
                     ILAGIPSVVFGLFGLSLFSITFGFGTSVLAGGLTLGIMSLPYIISNTHESLTAVPYSY
                     REASLALGANRAETSFRIVLPASFSRILTGVIIAIGRIIGETAPVLFTGAAFYITRNP
                     TGPFEPAMALPTHIFVLSAVYPDNVTPKLEGSISLLLIIVVVLFLLVAIRRRKETKKL
                     EV"
     gene            2438..3190
                     /gene="pstB"
                     /locus_tag="PLD15_10395"
     CDS             2438..3190
                     /gene="pstB"
                     /locus_tag="PLD15_10395"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPB64362.1"
                     /translation="MEDTIFVIDELNVFYGEKHALKDVTCEIPRRKVTAVVGPSGCGK
                     STFLRVLNRMNDLIPTYRHTGSIKLLGKETLTMDAVSLRKQVGMVFQKPNPFPKTIQD
                     NVTYGPRIHGVKNRQRLKEIAERSLKQAALWDEVKDELKKNALNLSGGQQQRLCIARA
                     LSVEPEVLLLDEPTSALDPIATQKIEKLIEELSERYTIIIVTHNLQQALRISDYLMFF
                     YVGELVEFDTTEKITTNPSDPRTSDYLRGIFS"
     gene            3206..>3718
                     /gene="phoU"
                     /locus_tag="PLD15_10400"
     CDS             3206..>3718
                     /gene="phoU"
                     /locus_tag="PLD15_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPB64363.1"
                     /translation="MTQERQRHLEAGFNRLKSMLSDMIENVRSSLVKAIDSLDRLDGD
                     SARTVIDGDQIIDDLQRKMDLEICNYIGRFQPLAEDLRYVLTMMKLATDLERIGDLAV
                     NIAEVAVRYENQTLVKPLVHIKKMTAVVENMLDEIIKAYYNRDIEVTRKVWSEDAKVD
                     EYYVYIKREVR"
BASE COUNT          969 a          901 c          955 g          893 t
ORIGIN      
        1 gaggccttca aggctctcaa acccggggtg gagatctcca tcgaaggcgc tggatcctcg
       61 actggtatca aagctctttt caacggtcag acagataccg ccaactccag caggtggatc
      121 aaaccttcgg aaatcgaaca gatgaacaga gacggaaagt acttcgttcc atacattgtg
      181 gcctacgacg gtatagccat catcgtcaac agatctctgg cgattgacaa cataacgctt
      241 caacagctct acgatatcta ttctgggaaa gtgacgacct ggaaccagat agattcatca
      301 cttcccaggg taaggatcgt tccgttctcg agggacaacg cttccgggac tttcgaatac
      361 tttatagagc atgtcatgaa aggtgataaa ctcgcaccac aggttcaaca gctcgcctcc
      421 accagggccg aagtagaaca ggtagttcag aacaattacg cgatcggcta cataggtatg
      481 ggttatatca caggagaggt caaggcgctc actgtcgaag gagtcgaacc gaccgttgca
      541 aacgttaatg caggaacata cccggtatcc agaccgctct tcatgttcgt gaatgccacg
      601 aatggacttc caacgggagt agtggccgat ttcctgagat acgctctttc gccggaaggt
      661 caggcagccg ttctcacagt tggatacgta aacgcatacg gcacgaaata agattggatg
      721 agtccggttg cgaagaaaga gagttgaact atcggcgaaa gttctggtga ctttcgccgc
      781 tttgctgaca tctattatag tcttcgggat cttttacttc cttatatctg acggggtgcg
      841 tgtatttgga agagtatcgc cgaaagactt cttattcagc gcccgctggt accccaccta
      901 cgaggatgcc gagtttggaa tcctcgcctt gctcgctggc actctgagcg ttacaggatt
      961 aactctggtt atagcgattc ctctgggttt tgtctgcgcg atctttctgg ccgagttcag
     1021 tggcaggaaa agccacgatg ccatcaaata tatcttcgaa ctcatggccg gcatcccgtc
     1081 cgttgtgctg ggaagtttcg gtctcaagta cgtctccaga tggctgatgg aacatttccc
     1141 tctccatgta tggaccgggc tgaacgttat gaacgcctct ttaatgctct ctatactcgc
     1201 gatgccgtac tttgttactc tcgtggaaga tgccctgaga gctgttccta gggatcagaa
     1261 agaggcttct ctagctctgg gtgcgaatat gacgggcacg attttcagga tactcttacc
     1321 tcaggcaagg agcggcatac tgaacgcgat catactggga accaacaggg ttatcggtga
     1381 gaccatggta gttctcatgg ttgctggagg agcgaccatg gtgccttcca cgatcttcga
     1441 tcccgtgagg ccgctcactt cggcgatcgc cggagagatg ggagaggtcg agctgggaag
     1501 cacacattat tttgcccttt tcatgatcgg tgttgtactt ctggcgattt cgactgtttt
     1561 cactctgata gccatgaagc taaaaaggag taaaggaaaa tgaagataga cgcaatcgtg
     1621 aagataacct tcgccgttgt ttcatacacc ttgatcggcc ttatagtggc tttgataggc
     1681 tttctgatag tcaacggttt ggacaatatc gatctggagt tcctgacggc tttcccaagg
     1741 aattcgatga ccgggggcgg gatctggccg gcgctggtcg gaacggttta cttcgtggcg
     1801 gtagctcttt tgttctctat acctgttgga atactggcgg ccgtttatct gagcgaatac
     1861 agcaaagata ccgccttcaa aagacttata ctcaccgcca acaacattct ggccgggatc
     1921 ccctcggttg ttttcggcct gttcggccta tcactcttct cgatcacttt cgggttcgga
     1981 acgtcggttc tggccggcgg tctcacactg ggaattatgt cgctccccta cataatatcc
     2041 aacacccacg aaagcctgac ggcagttccc tattcgtaca gagaagcatc cctcgcactg
     2101 ggcgcaaaca gggccgagac gtcttttaga atagtgctgc cggcctcttt ctctcgtatt
     2161 ttaaccggag tgattatagc gattgggagg ataataggcg aaacggcacc cgtactcttc
     2221 accggagcgg ccttttatat aaccaggaac cccaccggtc ccttcgaacc ggccatggcc
     2281 ttaccgacac atatattcgt tctttcggcc gtctatcccg ataatgtgac ccctaaactc
     2341 gaaggtagca tatctctcct cttgatcata gtggtggttc tcttcttgct tgttgccatt
     2401 agacggagaa aggaaacgaa gaagctggag gtgtaaggtg gaagatacga tcttcgtgat
     2461 agatgaattg aacgtattct acggtgaaaa gcatgcattg aaagatgtga cgtgcgagat
     2521 accccgaagg aaggtgactg ccgtcgtcgg tccttccggt tgtgggaaat cgacctttct
     2581 cagggtgttg aacaggatga acgatttgat cccgacttac agacacaccg gttccatcaa
     2641 acttctcggc aaagaaacgc tcacgatgga cgcggtctct ttgagaaaac aggtggggat
     2701 ggtctttcag aagcccaatc cctttcccaa aaccatacag gataatgtaa cgtacggacc
     2761 gagaatacac ggtgtgaaaa accgccagag actgaaagag atagccgagc gatccctcaa
     2821 gcaggcggcg ctctgggatg aagtgaagga cgaactaaag aagaacgcgc tgaacctctc
     2881 aggaggacag caacagaggc tctgcattgc aagggcgctg tcggtggaac cggaagtcct
     2941 gctgcttgac gaacctacca gcgctctgga ccccatcgca acgcagaaga tagaaaaact
     3001 tatcgaagaa ctctctgaaa ggtatactat aataatagtt acccacaatt tgcagcaggc
     3061 tctgaggatt tcggattact taatgttctt ctatgtcggc gaactggtcg aattcgatac
     3121 aaccgagaag ataactacta acccgtcaga tccgagaacc agcgactatt tgaggggtat
     3181 tttcagctaa cggggagtgg taagcatgac tcaagaaagg cagcgccacc ttgaagcggg
     3241 tttcaacagg ttgaaatcga tgctatcgga catgatagag aacgtcagat caagcctcgt
     3301 aaaggcgatc gattctttag acagactcga cggagactcc gccagaactg ttatagacgg
     3361 tgaccaaatt attgacgacc ttcaacgcaa aatggattta gaaatatgca actatatagg
     3421 gaggttccag ccccttgccg aagacctgag atatgtcctg accatgatga agctcgccac
     3481 cgatctcgag agaataggag acctggccgt caacatcgcc gaagtcgccg tgaggtacga
     3541 aaatcagacg ctggtaaagc cgttggtcca catcaagaaa atgaccgcag tggtagagaa
     3601 catgctcgat gagatcatca aggcctacta taacagagat atcgaagtta ccaggaaagt
     3661 ctggagcgaa gatgccaagg tcgatgaata ctatgtttac ataaagcgcg aggttcgc
//
LOCUS       DAOOLY010000248         3692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_30296_length_3692_cov_3.526258, whole genome shotgun sequence.
ACCESSION   DAOOLY010000248 DAOOLY010000000
VERSION     DAOOLY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30296_length_3692_cov_3.526258"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLD15_10405"
     CDS             <1..263
                     /locus_tag="PLD15_10405"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPB64364.1"
                     /translation="MGVAREMARIILPISMYTEAYWTINLRSMMNFLNQRADSHAQYE
                     IQKYALAIAGILKEKCPISYEAFLKYRYEGDLLVGGVANETV"
     gene            250..615
                     /locus_tag="PLD15_10410"
     CDS             250..615
                     /locus_tag="PLD15_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64365.1"
                     /translation="MKLSEIVQLLNCETLNSVDDSNPEIASVGAADLMSDVLAFGGPG
                     MILVTGLNSPQCVRTASVVGASAVLIIRKRNVPETTIELAKELQMPLLHARMSMYKAC
                     GILFEKQISDALEEGKQID"
     gene            608..1567
                     /locus_tag="PLD15_10415"
     CDS             608..1567
                     /locus_tag="PLD15_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB64366.1"
                     /translation="MTERNVDDLLEKLRGLFSDITAKDIMVTNIISLTKDRTMWQAKE
                     LMRICKISGVPIVDNDNHLLGLVSIEDIILALEGNHITDPIEKHMTKDLITFAPDVDL
                     EGLIERFNRYRYGRFPVVDSEGRLLGIISKKDIIGAILDKFRIIYVHDARRKEVLERN
                     VDWFDRSLISGDYVEKKSADFLFHIDYTEVDMAGVGSAKLKSYLKTRTDDDSLVRRVS
                     IACYEAEVNVVIHSGSEGYIYAWYDESVIRVRVEDYGKGIENVEQAMKEGFSTASDHV
                     RELGFGAGMGLPNMHRYSDKMVVVSEARKGVVVEMNFYRNGRK"
     gene            1569..1904
                     /locus_tag="PLD15_10420"
     CDS             1569..1904
                     /locus_tag="PLD15_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HPB64367.1"
                     /translation="MRLKELIEKCELKVVTTLPLDAEVTGGYIGDLLSDVMGNAPAHS
                     IWLTVQSHTNILAVASIVGIKAIVLCNDLHFEEVTRKKAEENSIVLLESAETSFEVTT
                     RLIKSGLKG"
     gene            1909..2634
                     /locus_tag="PLD15_10425"
     CDS             1909..2634
                     /locus_tag="PLD15_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB64368.1"
                     /translation="MESFFCDFHIHSCLSPCADITMTPNEIARVCCERGIDWIAITDH
                     NSAGNVRVFTEVLKRLGIAVIPGMEIHTLEDVHVLAYFPDISVAEEYSRWLLDEKLNR
                     IEIDPEISGYQLYAGEDDSFTSMENIWLGQPALLGIDEAISTVEERGGVCVLAHVDRK
                     MGLVIQLGFIPENYRDIPVEVSFRESLKKIDLKTDNIIHSSDAHSLNLVRPTISMMAE
                     KRIFNEFKAALTSGGKRVKIIWD"
     gene            2625..3188
                     /locus_tag="PLD15_10430"
     CDS             2625..3188
                     /locus_tag="PLD15_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB64369.1"
                     /translation="MGLRTLCDHVVDIAQNAVESGTEKAWLDIEEQRDSTFRFTVRDA
                     GRGIPEELQESVLDPFYTQKRKKVKFGLGLPMLKFAALSTGGEFELKSSLGKGTTVSS
                     LFMLSNVDCQPVGDVAMSLFSIITMNGEIDWYIQRSLDDDGYAVESSQFREALGNNCF
                     TTPVKMKMILDLLQEVENSLGGYSDVR"
     gene            3178..>3692
                     /locus_tag="PLD15_10435"
     CDS             3178..>3692
                     /locus_tag="PLD15_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64370.1"
                     /translation="MSVKKRVLTFLFAALFCFAFGSIVPSRIAILDNRVVSDGEVLEI
                     LKVERNIEISESDLKEAVARLEASGYFSSIIYQYDESSRLLTIQVKEYPVVEVVVAFE
                     GPKIVEKETLEATSTIKSGKPLNPSRLMFEIPMTLEAMEGVLLSKGYIPPFATIEWTT
                     DKDRADIVSGK"
BASE COUNT         1020 a          815 c         1004 g          853 t
ORIGIN      
        1 cgatgggggt ggccagagag atggctcgta ttatactccc gatatcaatg tacacggagg
       61 cttactggac gataaacctg agatctatga tgaatttcct gaaccagagg gccgattcac
      121 acgcgcagta cgaaatccag aaatacgcgc tagcgattgc aggtatactc aaagagaagt
      181 gtccaatttc atacgaagcc ttcctgaagt accgctacga aggagatctg ttggttggag
      241 gggtagcgaa tgaaactgtc tgaaatagtg caactcctta attgtgaaac tttgaacagc
      301 gttgatgatt ctaatcccga aatagcatct gtgggagcgg ctgatctcat gagcgatgtc
      361 ctcgctttcg gcggtcccgg aatgatcctc gtgaccggac tcaactcgcc ccagtgtgtc
      421 aggaccgctt cggtcgttgg cgcgtcggcc gttttgataa tcaggaagcg aaatgttccc
      481 gaaacgacga tagagctggc taaagaactc cagatgccgt tgcttcacgc ccggatgtca
      541 atgtacaaag cctgtggtat ccttttcgaa aaacagataa gcgatgctct cgaggaaggg
      601 aagcagattg actgagagaa acgtcgatga tcttctcgag aaactgaggg gattgttcag
      661 cgacataacc gcaaaagaca taatggtaac caacataata tctctcacca aagatagaac
      721 tatgtggcag gccaaagaac tgatgaggat atgcaagata tcgggagttc cgatagtcga
      781 taacgacaat catctgctcg gtctggtcag tatagaggat ataattctcg ccctggaagg
      841 aaaccatata accgatccta tcgaaaaaca catgaccaaa gatttgataa ctttcgctcc
      901 cgacgttgat cttgaaggac tcatagagag gttcaaccgt tacagatacg ggcggtttcc
      961 ggtggtggac tcagaaggaa ggctgttagg gattatctcg aagaaggaca taataggtgc
     1021 gattctcgac aagttcagaa tcatatacgt ccacgatgcc cgccgaaaag aggtcctgga
     1081 aagaaacgtg gactggttcg acagatcgtt gatctccgga gactacgttg agaagaagtc
     1141 ggccgatttt ttgttccaca tcgattatac agaggtggac atggccggcg tcggttcagc
     1201 aaagctgaag agttacttga aaaccagaac cgacgacgac agtctggtca gaagggtatc
     1261 gatcgcctgt tatgaggccg aagttaacgt ggttatccac agtggaagcg aaggatacat
     1321 atacgcctgg tacgatgaat cggtcatcag ggtgagggtc gaagattacg gaaagggaat
     1381 cgaaaacgta gaacaggcta tgaaggaagg cttttcgacg gcttccgacc atgtgagaga
     1441 gcttgggttc ggtgctggaa tgggactgcc gaacatgcac aggtattccg acaaaatggt
     1501 agtggtctcg gaggctagaa agggtgtggt cgttgagatg aatttctacc gaaacgggag
     1561 gaaatgaaat gaggctgaaa gaactgattg aaaaatgtga gctgaaagtg gtcacaactc
     1621 ttcctcttga cgcagaggtg accggtggtt acataggcga tctcctgagc gatgttatgg
     1681 ggaacgctcc tgcccattct atatggctca cagtgcaatc tcacaccaac atactggcag
     1741 tggcctcgat cgttggcatc aaagcaatag ttctctgcaa cgaccttcac ttcgaagagg
     1801 ttaccagaaa gaaggcggaa gagaatagca tcgtgcttct ggaaagcgcg gagacatcgt
     1861 tcgaagtgac aacccggcta ataaaaagcg gattgaaagg ctgactcttt ggaatccttc
     1921 ttctgtgatt ttcatattca cagttgtctt tcgccgtgcg cagatatcac aatgactcct
     1981 aatgaaatcg cgcgcgtctg ttgtgagagg ggtattgact ggatagccat aacagaccac
     2041 aacagcgccg gcaacgtcag ggttttcacg gaggtgctga agagattggg tatcgccgtg
     2101 attccgggta tggaaatcca cactctcgaa gatgtgcatg ttctagctta ctttcccgac
     2161 atctccgtcg cggaggaata ctcccggtgg ttgctggatg agaagctgaa cagaatcgag
     2221 attgatcccg agatctccgg ctatcagctt tacgcaggtg aagacgactc tttcacctct
     2281 atggaaaaca tctggctggg ccagccggcg cttctgggta tcgacgaggc gatctccacg
     2341 gtggaagagc gcggaggcgt ttgcgttctg gcccacgtcg atagaaagat gggcctggta
     2401 attcaactgg gcttcatacc ggagaattac agggatattc cggtggaagt ttccttcaga
     2461 gaaagtttga agaagatcga tctcaagacc gataacataa tccactcgag cgacgctcac
     2521 agtttgaacc tcgtgagacc tacgatttcg atgatggccg agaaaaggat tttcaacgag
     2581 ttcaaagcgg cgctgacctc aggaggaaag cgtgtgaaga taatatggga ctgaggactc
     2641 tatgtgatca tgttgtagat atcgctcaga acgccgttga atcggggact gaaaaggcgt
     2701 ggctggatat cgaggaacag agggacagta ccttccgctt cacagtccgc gatgccggaa
     2761 ggggtatacc cgaggagctt caggaatcgg ttctcgatcc cttctatact caaaagcgta
     2821 agaaagtcaa attcggactg ggacttccta tgctgaaatt cgccgcactc tctacgggcg
     2881 gagaattcga gctgaagtcc agtctcggaa agggtacgac cgtgagctcc ctcttcatgc
     2941 tatcgaatgt cgactgtcag cccgtcggcg atgtcgctat gtcgctcttt tcgataataa
     3001 ccatgaacgg agagatagac tggtacatac agaggagtct agacgacgac ggatacgccg
     3061 ttgaatcgtc acagttcaga gaggctctcg ggaacaactg tttcacaact ccagtcaaaa
     3121 tgaagatgat cctggatctt cttcaagaag ttgaaaattc gctgggaggt tattcagatg
     3181 tccgttaaaa agcgagtcct tacttttttg tttgctgcac tgttctgttt tgccttcggg
     3241 agcatcgttc cttcaagaat cgcgattctc gacaacaggg tagtttccga cggtgaagtc
     3301 ctggagattt taaaggtcga gagaaacata gaaatctcgg aatccgacct gaaagaggcc
     3361 gtcgcaagac ttgaagcgag cggttacttt tcttcgatca tttaccagta cgatgaaagc
     3421 tcaaggctcc tgaccataca ggtgaaagag tacccggtcg tggaggtcgt ggtggctttc
     3481 gaaggcccca aaatagtgga gaaggagacg ctggaagcta cctctaccat aaaaagcggg
     3541 aaaccgctta acccttcgag actcatgttc gaaatcccca tgacgctcga agcgatggaa
     3601 ggggttcttc tcagcaaagg ttacataccg cccttcgcca cgatcgaatg gacaaccgac
     3661 aaagacaggg ccgatatagt ctcggggaag at
//
LOCUS       DAOOLY010000249         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_30487_length_3675_cov_2.984530, whole genome shotgun sequence.
ACCESSION   DAOOLY010000249 DAOOLY010000000
VERSION     DAOOLY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30487_length_3675_cov_2.984530"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2494)
                     /locus_tag="PLD15_10440"
     CDS             complement(<1..2494)
                     /locus_tag="PLD15_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB64371.1"
                     /translation="MWRRLLGELSRRPVAVFLLMGTLIFLGLFSWQKLPVDLLPNLNV
                     PYALIVTPYIGATPGEVERDITDPLESAVSFASGVKSVSSQSRDGYSILTVEFEGDTD
                     MAFAVSRLRDYIDTFSFELASDVDPIIVEFNPSLLPVYILGISSREAKDLQAHTNKLM
                     GALSRIDGVANVMLAGIPEQQVVVTLDRERMEELEIPLEIVKTVLEEGVRYPMGFLEN
                     DGLVYTLSVRCEFTSLEELSNTVVGFRGMGSSISALTLETGQGFRLDGIPIPVRLNQI
                     AVVELEDQEIRGAISVNGQSAAVLTVQKQSGANTVNVAAEISEILKEWEKSETQSEVI
                     TISDTSQFTNMAINGLFRNLLIGALVATLVIFAFLRNFVATLAIAVSMPLSLLTAILI
                     LYFSGLGLDLMTLGGLTMAVGMIVDNSIVILEAIYRYLEARKRPYEAAGEGSEVVGAI
                     FASTLTTVAVFVPFAFISGLAAQIFRYFAFALAFSLGASLFIAIVMIPAFTGFIRVRR
                     PRESNYVRYKAFLKKLLERRFLTVLVFIAIFVISLFGFFLRDTEFIPSFDNGIVTIDI
                     TLPKNTDYGTTVKAAKEIEASILGNSSELALKTVYTMAGVTGDIIALLAGSSENTATV
                     QVYLKDLGERRISTEDALRILSKITDGIVEKYSGEISIGTSGLELEAVFGRDIEIAVF
                     DGDLDSMKARIEAFAIELAGLEGVENIQTSFDNRQDVLELTIDRSKATLAGLYHLQVL
                     AAIQPYTIGIDLGKISLNGRSLPVKLQQVKGEGYEWIKSLPIDSLLGSKTYVGVVSSM
                     EIRESPSTIEHFDNQRVGYVKADVLGRPLGQVV"
     gene            2860..>3675
                     /locus_tag="PLD15_10445"
     CDS             2860..>3675
                     /locus_tag="PLD15_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB64372.1"
                     /translation="MCDSVVDLPEELARRDDVFVVPVMVILNGKSYREGQEIEKKAIL
                     QHLERNFAHTSLPNPTDVLESYRSMVERGYNELLVINLSGSLSGTHNLFATMAAQFMS
                     ENPNVHIECIDSLSLSGGAAMLVYRAFMMKERGDSLKVIASDLRKRAGKDNIVFFVLP
                     TLKYLKESGRIGKLEAALGQILNVKPVVTIDRSGVFSQVGRTRGMKKAVEKMVERLKS
                     AVNGKKIFCIALYHSGNDNETMTLVSWVKEQISGLSKVLFAGELSAGILVHGGS"
BASE COUNT          954 a          924 c          833 g          964 t
ORIGIN      
        1 ctactacctg tccgagtggt ctacccagta catcggcttt cacatagcca acccgctggt
       61 tgtcaaagtg ctctattgtt gaggggcttt cccttatctc catgctggag actacgccga
      121 cataggtttt gcttccaagc aaagaatcga tcggaaggga tttgatccat tcatacccct
      181 ctccctttac ctgttgaagc ttaaccggca aactccttcc gttcaggctg atcttcccaa
      241 ggtcaatacc tatcgtgtag ggctgaatgg ccgccagaac ctgtaagtgg taaaggccgg
      301 caagtgtggc cttgctcctg tctatagtca actcgagtac atcctgtctg ttatcgaaac
      361 tggtctgtat attctcgaca ccctccaagc cagcaagctc gatggcaaaa gcttcgatcc
      421 tggccttcat actatcaaga tcgccatcga atacggctat ttctatgtct ctgccgaaga
      481 cggcctccag ttccagaccg ctcgtgccga tgctgatctc tccgctatat ttttcaacaa
      541 ttccgtctgt gatcttcgat aaaattctca acgcatcctc tgtggaaatt cgcctctcgc
      601 cgagatcctt cagatagacc tggacagtgg ccgtgttctc cgacgaacct gccaggagag
      661 ctattatatc cccggtaaca ccggccattg tatagaccgt tttcaaagcg agttcggacg
      721 aattaccgag tatagaggct tctatctctt ttgccgcctt tactgtggta ccataatcag
      781 tgttttttgg tagtgttata tcaatggtaa cgatgccgtt gtcaaagctt ggtatgaact
      841 cggtgtccct taaaaagaag ccgaatagac ttataacgaa tatcgcgatg aataccagca
      901 cggttagaaa tctccgctct agcaactttt tcaaaaaagc tttatatctt acatagttgc
      961 tttccctggg tcgcctgacc cttatgaatc ctgtgaaggc tggaatcatg actatagcaa
     1021 tgaacagcga agcgcccaga gaaaaggcca gtgcaaacgc aaaatatctg aagatctgag
     1081 cggctagacc gcttatgaag gcgaaaggca cgaaaacggc tacggttgtc aatgttgagg
     1141 cgaagatagc accaacaact tcggagcctt cgccagccgc ctcataaggt ctcttcctgg
     1201 cttcgaggta tctgtatatg gcctctagta tgactatgct gttgtcaact atcattccta
     1261 cggccatggt gagacctccc agggtcatca agtccagccc caaaccggaa aagtagagta
     1321 tcagtatcgc agtcagcaga gagagaggca tcgaaaccgc tatggctaga gtcgcaacga
     1381 agttcctcaa gaaggcgaag atcaccaggg tggccacgag cgctccaata agcagattcc
     1441 taaaaagccc gttgatagcc atgttggtga attggctcgt atcactgatc gttatcactt
     1501 ccgattgtgt ctccgatttc tcccactctt taagtatctc cgagatttcg gcggcgacat
     1561 tcacagtgtt ggctcccgat tgcttttgaa cggttagaac agctgcactt tgaccgttaa
     1621 cggagatcgc accccttatc tcttgatctt ccaactccac aacggctatc tggttcaacc
     1681 tgacgggaat agggattcca tcaagccgga agccctgacc cgtctccagt gtcagggccg
     1741 atatcgaact ccccatacct ctgaatccca ccaccgtgtt cgaaagctct tccagcgaag
     1801 tgaactcgca tcggaccgaa agagtgtaca ccaggccgtc attctccaga aaacccatgg
     1861 gatagcgcac gccttcttcc agaacggttt ttactatctc cagcggtatc tccagctcct
     1921 ccattctctc tctatctaga gtgactacaa cctgctgctc ggggataccg gcgagcatca
     1981 cgtttgctac tccgtcgatt ctcgaaagcg ctcccatgag tttgtttgta tgagcctgga
     2041 gatcctttgc ctctctggaa gagattccca gtatatacac cggtagaagc gagggattga
     2101 attcgactat tatgggatct acatcgcttg caagctcgaa ggaaaacgta tctatgtagt
     2161 ctctcaatct tgataccgcg aaagccatgt cggtatctcc ctcgaactcg accgtaagta
     2221 tcgagtatcc gtcacgcgat tgcgatgaga cactcttcac gccgctggca aaggaaacgg
     2281 cgctttctag aggatcggtg atatctcttt caacctcccc gggtgtcgct cctatgtacg
     2341 gagtcacgat cagagcgtag ggaacgttca gattgggcag aaggtcgacc ggcagtttct
     2401 gccaggaaaa aagccccagg aatatcagtg ttcccatcaa aagaaaaaca gcaacaggtc
     2461 ttctggatag ttctccaagc aatcttctcc acattgaact cacccgataa tcactctcta
     2521 aacacaaatc atacccgttt aatactttgg aaccccgtaa cgaaagtcat aacgtaaagg
     2581 atctcttctt acacctcaaa atggttttaa gcatcatagt ttcgtatcaa tagtatattt
     2641 tactatctgt accatcgaga atccaaaacg gaagcttctg tttatcgatc ccttcattct
     2701 tccaaaccaa aaccgtgata atcgttcatc tatcggtcgg gagacggggt ataatctttt
     2761 tagtgaagat ttttttgaac catgcgtttc cacagtaacg attctatggt tcgccaattt
     2821 gtcaaatccg ggaggggttg atccgtcttg ataggttttg tgtgtgattc agtagtggat
     2881 cttccagaag agctggccag gcgggacgat gtttttgtgg taccggtcat ggtgattttg
     2941 aatggaaagt cttatcgtga gggtcaggaa atcgaaaaga aagcaatact acagcatctg
     3001 gaaaggaatt ttgcccacac ttccctacca aatcctacag atgtactgga atcttaccga
     3061 tcgatggttg aacgcggcta caacgaactc ctagttataa atctctcggg ttctttaagc
     3121 ggtacacaca atctcttcgc cactatggcc gctcaattca tgagcgagaa tccgaacgtt
     3181 cacatcgagt gtatagattc actttcgctc tctggtggag ccgccatgct tgtctatcgg
     3241 gcctttatga tgaaagagag gggcgattct ctgaaagtga tcgcctccga tctcagaaag
     3301 cgcgccggta aggataatat cgttttcttt gtacttccca ccctgaaata cttgaaagaa
     3361 agtggaagaa tcggtaaact ggaagcggct ctgggacaaa tacttaacgt taaacccgtt
     3421 gtcaccatcg accgaagtgg ggttttttcc caggtaggaa gaaccagagg aatgaaaaaa
     3481 gccgttgaga aaatggttga aagacttaaa tcggccgtta atggcaaaaa gatattttgc
     3541 atcgcgctat accattccgg caacgacaac gaaaccatga cgctggtctc ctgggtgaaa
     3601 gaacagataa gcggtctttc aaaggtgctt tttgcgggtg agctgtcggc cggaattctc
     3661 gtgcacggag gaagt
//
LOCUS       DAOOLY010000250         3672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_30524_length_3672_cov_4.384020, whole genome shotgun sequence.
ACCESSION   DAOOLY010000250 DAOOLY010000000
VERSION     DAOOLY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30524_length_3672_cov_4.384020"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLD15_10450"
     CDS             <1..822
                     /locus_tag="PLD15_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64373.1"
                     /translation="VVQVIFIYLPLAWAFYVSFHSWNMIRPMKWVGLENYIRMFETPD
                     FWGSLWTTVLYVLGTVVPSVILGLLIAMLLNIEWLKGKGIFRTLFYIPVVTSMAAAAV
                     IWGWLYEPNFGLVNYFLSWFGIKSIKWLSDPNYALLGLIIVGVWKRVGYNMVLFLAGL
                     QTIPRTYYEAAEIDGATSWDKFRSITLPLLSPTTLFVVIMQFIASFRVFVSVSVMTRG
                     GPAKSTQVITYYLYENAFRYLKFGYASAIAVFMFALMVVLTLIQFAISKKRVHYS"
     gene            819..1649
                     /locus_tag="PLD15_10455"
     CDS             819..1649
                     /locus_tag="PLD15_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64374.1"
                     /translation="MRKKPHIVLRTTVILLVLFVAILINLPFIWMLVTSFKTEDAAFT
                     IPPSFLPTIFDFRNFVKAFELIPLSQYVVNTLFVALAVTLLQLVFNSLAAYGLARIRF
                     KGSSIVFMVLIGTLMVPPEVTMVPLYVIVKQFGWINQYKALIVPFMSSAFGIFLLRQF
                     FMGIPRELEEAAIIDGASRMRIFLKIIIPLSKPALYTMSLYTFLAHWNEYMWPLIVIN
                     DAKKQMIQVGISQFVSGWETQWTMRMAASTTAVLPIIVFFFFVQRQFVEGISISGLKE
                     "
     gene            1677..2921
                     /locus_tag="PLD15_10460"
     CDS             1677..2921
                     /locus_tag="PLD15_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64375.1"
                     /translation="MRKILLAVLFIAAVALAFAAPVKVTMWYAQTGIYSQTLLDIVAD
                     FNKLQEGKIEVEAVYTGSYQDTMQKLLAALVAGDVPTLAQIEQARIGQFVDGGAFQDL
                     NEFIKKDTEFAAMLDDFWPRFIAANTYEQGLLGFPLNCSTPLMYINRDLFKQAGLDPD
                     NPPKTWTEVYAASKQIKALGPDIFGYRFGIDDWLLEAYIWQFGGQIISEDGTKMLIYS
                     PETVNAWNFFQKGVREGIFIFGTTGGNELDLSGRIAMVVRSTGSLGYLIQNAKFDLGA
                     TIMPMQEKLVVPIGGANVFMFSSRPKAEKEAAWEFLKFLTSTANTAKWSLATGYMTSR
                     ISAFESPEIQKIMTDDPRFALTYQQLRDSAVRRPWYGPYPEVHAMITSAWESVMADPT
                     KDVDAVLKQLHKDAQRVLDDYY"
     gene            2984..>3672
                     /locus_tag="PLD15_10465"
     CDS             2984..>3672
                     /locus_tag="PLD15_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HPB64376.1"
                     /translation="MKRVFVVLFSLISLAVCGSVFFGNIHAHTSYSDGRGTPDQAFRH
                     ARDSGAVDIQAITEHCHDLVYPLPDGSMKLDVVRNLAREYTREGKFIAISGYEWTLTS
                     RGHITIYNTEKFLDRSRSDLLDIYAWIVEKKATAQFCHPGRTYGDFFDFLYFPEVDLY
                     INTIEVGNGAGKSNNVIKPEYLERYQRALNRGWHVGASANQDNHYENWATANDARTAF
                     VIDELTTEKVY"
BASE COUNT          869 a          944 c          943 g          916 t
ORIGIN      
        1 gttgttcagg tgatttttat ctatcttcca ctcgcctggg ctttctacgt ctcgtttcat
       61 agctggaaca tgattcgacc gatgaagtgg gtcggcctgg agaactacat aaggatgttt
      121 gagactccgg atttctgggg ctctctctgg acaacggttc tttacgtact gggcacagtt
      181 gttccttctg ttattttagg tcttcttatc gctatgctgt tgaatatcga gtggttgaag
      241 ggaaagggga tcttcagaac tctcttctat attcccgtcg taacctcgat ggccgccgcg
      301 gccgtcatat ggggatggct gtatgagccg aacttcggac tggtgaatta cttcctctca
      361 tggttcggca taaaaagtat caagtggttg agcgatccca attacgctct gctcggactg
      421 ataattgtcg gcgtctggaa acgtgtgggc tacaacatgg ttctctttct ggccggactc
      481 cagaccatac ccaggactta ctacgaagcc gccgagatcg atggcgccac ctcctgggat
      541 aagttcagga gtataactct tccgcttctt tctccgacca cgttgttcgt tgtcatcatg
      601 cagttcatcg cctctttcag ggtcttcgtt tccgtatcgg tcatgacgcg cggtggaccg
      661 gccaagagca cacaggttat aacctactac ctgtacgaaa acgccttcag gtatctcaag
      721 ttcggttacg cctcggccat cgccgtgttc atgttcgcct tgatggtcgt tctcaccctc
      781 attcaattcg cgatcagcaa aaagagggtg cattactcat gagaaaaaaa cctcatatcg
      841 tattgagaac cacagtgata ctactcgtat tgttcgtcgc gatactgatc aatcttccct
      901 tcatatggat gctggttact tctttcaaaa cggaggacgc ggctttcacg ataccgccca
      961 gttttctgcc gacgatcttc gatttcagga acttcgtcaa agcctttgaa ctgataccac
     1021 tcagtcagta cgtggtcaac actctcttcg ttgcactggc agtgactcta ctgcagctgg
     1081 tcttcaattc gctcgcagct tacggactgg cgaggataag gttcaaaggc tctagtatag
     1141 tcttcatggt tttgatcgga acgctcatgg ttccgccgga agttaccatg gtccctctct
     1201 atgttatcgt aaagcagttc gggtggataa accagtacaa agctctgata gttccgttta
     1261 tgagttcggc cttcggtatc ttcctgttga ggcagttctt catgggaata cccagggaac
     1321 tcgaagaggc ggccataatc gacggcgcca gcaggatgag gatctttttg aagatcatca
     1381 tacctctgtc gaaaccggcc ctgtatacga tgtctcttta taccttcctc gcacactgga
     1441 atgaatacat gtggccgctc atagtgataa acgacgccaa gaaacagatg attcaggtcg
     1501 ggatttcgca gttcgtcagc ggctgggaga ctcagtggac gatgaggatg gctgcttcga
     1561 ctacggccgt tttaccgatt atagttttct ttttctttgt tcagagacag ttcgtcgaag
     1621 gaataagcat ctcgggtttg aaagagtaac ccaaatcttg aaagggaggt tcttttatga
     1681 gaaagatttt gctagccgtt ctgttcatcg cggcagtcgc cctggctttt gcggcgcctg
     1741 ttaaggtaac gatgtggtac gcccagacgg gtatctattc gcagactctt ctggatatcg
     1801 tggcagactt caataagctc caagagggca agatcgaagt agaagccgtc tataccggaa
     1861 gctaccagga tacgatgcaa aagttgctcg cggcgctggt cgccggagat gttccgaccc
     1921 tcgctcagat cgagcaggcg aggataggtc agttcgtcga tggcggagcc ttccaggatc
     1981 tcaacgaatt catcaagaag gatacggaat ttgcggcgat gctcgacgat ttctggccga
     2041 ggttcatagc tgccaacact tacgagcagg gactattggg cttccctctc aactgcagca
     2101 ccccgttgat gtacatcaac agggacctct tcaagcaggc cggtctagat cctgacaacc
     2161 ctccgaagac ctggaccgaa gtttatgccg cgtccaagca gatcaaggca ctgggtcccg
     2221 atattttcgg ttacaggttt ggcatcgacg actggcttct cgaggcctac atatggcagt
     2281 ttggcggaca gatcatatcg gaagacggca caaaaatgct gatctactct cccgagactg
     2341 taaacgcctg gaatttcttc cagaagggcg tcagagaggg aatcttcata ttcggaacga
     2401 ccggcggaaa cgagctggac ctttccggta gaatagcgat ggtcgttcgc tctaccggaa
     2461 gcctcggtta cctcatacag aacgccaagt tcgatctggg cgccacgata atgccgatgc
     2521 aggaaaagct cgtggttccg atcggcggtg caaatgtctt catgttctcc tcgagaccga
     2581 aagccgagaa agaagctgcc tgggaattcc tcaagttcct cacctcgacc gcgaacacag
     2641 cgaaatggtc gctcgcgacc ggttacatga ccagcaggat ctcggccttc gaatcgcccg
     2701 aaatacagaa aatcatgacc gacgacccga gattcgccct cacataccag caactgagag
     2761 acagcgctgt aagaagaccc tggtatggtc cttatcccga ggtacacgcc atgatcacgt
     2821 cggcctggga atcggtcatg gccgatccca ccaaggatgt agacgcagtt ctcaaacagc
     2881 tccacaagga tgcacagaga gttctcgacg attactattg atctttattc agcgacgaca
     2941 ggggcagtct caggctgccc ctgttttttg agaggtgttt gaagtgaaga gagtatttgt
     3001 cgtattgttt tcattgatct ctctggccgt ttgcggttca gtctttttcg gcaacatcca
     3061 cgcgcacacg tcttactccg atgggcgtgg aacgcccgac caggctttca ggcatgccag
     3121 agactctggc gcggtagaca tacaggccat aaccgagcac tgccacgatc tggtctatcc
     3181 ccttcccgac ggctccatga agctcgatgt cgtccgcaac ctggctagag agtacacacg
     3241 ggagggtaag ttcatagcga tatcgggtta cgagtggaca cttaccagtc gcggccacat
     3301 tacgatctac aacaccgaaa agttcctcga caggagcagg tcggacctcc tggacatata
     3361 cgcatggata gtcgagaaaa aggctacggc ccagttttgc catccgggaa gaacctacgg
     3421 agatttcttc gattttctgt actttccaga agtggatctc tatatcaaca ccatcgaagt
     3481 gggcaacggt gctggaaaat cgaacaacgt gatcaagccg gaatacctgg aacgttacca
     3541 gagggctctg aacagaggct ggcacgtcgg cgcctcggcc aatcaggaca accactacga
     3601 gaactgggcg acggccaacg acgcgagaac ggccttcgtg atcgacgagc tgactaccga
     3661 gaaggtttac ga
//
LOCUS       DAOOLY010000251         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31058_length_3624_cov_4.389185, whole genome shotgun sequence.
ACCESSION   DAOOLY010000251 DAOOLY010000000
VERSION     DAOOLY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31058_length_3624_cov_4.389185"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLD15_10470"
     CDS             complement(<1..348)
                     /locus_tag="PLD15_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB64377.1"
                     /translation="MNFKETLEKVLDEISSILSGVTDESVESLALKILGAERIFLFAM
                     GRSGLAIKAFAMRLMHLGLKVHVVGEISTPSIGEGDLLIVGTGSGETPSVVLNCRKAR
                     ELGAEIAAITASIE"
     gene            complement(345..995)
                     /locus_tag="PLD15_10475"
     CDS             complement(345..995)
                     /locus_tag="PLD15_10475"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HPB64378.1"
                     /translation="MLLQLANDSHIRSDFFTLAIKVASHVDIIEVGTPALLAHGMELV
                     RETKAAFPGHKVLADAKIVDGGYLEAAMAFQSGADIVTVLAFAPVKTIQETVKAAEEF
                     GGQIMLDTLAIESIEEFASKVAPFSPDYVCLHTSADLSDVGEDSHLTGYAEKMGTLRR
                     ILPHTMIAVAGGISLDNLPVLLSLRPDVVIAGRSISRAENPVEAAATMKKRMAGSI"
     gene            complement(999..1400)
                     /gene="rbsD"
                     /locus_tag="PLD15_10480"
     CDS             complement(999..1400)
                     /gene="rbsD"
                     /locus_tag="PLD15_10480"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HPB64379.1"
                     /translation="MKKNGILNKEICNLIGSMGHTDLMVISDSGLPIASDRYRIDVSI
                     IGGKPGIFNVLEPVLEELEIEKVIFSQEMKTVSPKTLEETIKRLPEGIEIEFVPHTKF
                     KELTNTQSKGVIRTGEQIPYSSVILVAGVTY"
     gene            complement(1414..2163)
                     /locus_tag="PLD15_10485"
     CDS             complement(1414..2163)
                     /locus_tag="PLD15_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB64380.1"
                     /translation="MLFNSDRRIVLDRASPVPLYYQIYRIISDYMKEPGVVGRKLPTE
                     EAMMKVFDVSRATIRRALQALESSGQIARRRGRGTIVTNNASQEHLTTIRSFTEQMRL
                     ENHVPITQVVDVRKVLADSELAKRLGVTEGEELLQVTRLRGNESYFPLALFISYLTPG
                     SRLTGEENFEGSLYELMRSKGSPVVDGDAIIEGRLAEGKIAELLKVENGAPILYYERL
                     GSTINNEPVELVQCWYEARHYKFRIHLSTGK"
     gene            complement(2229..>3624)
                     /locus_tag="PLD15_10490"
     CDS             complement(2229..>3624)
                     /locus_tag="PLD15_10490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB64381.1"
                     /translation="NQVEQDPEEWWESVKITTSELLSKAGVDTQEIKAVSFSGQMMGA
                     IPVDRNGKTLRRAIIWADQRSVEQAARLEKLGNDTVYRLTGSRITPTYSGPKIAWIRD
                     NEPETYRRAHKFLQAKDYIISRFTGEFGTDPTDASMTLLFDIKNWKWSERLADTLGLD
                     LEKLPEVFPSTSVVSTVLPVVARELGLSEKTLVIRGGGDGACACLGAGVVSPDEAYLY
                     LGSSSWISTCSREPVFDEKSRTFNFAYPIEGYYCPCGTMQAGGASYHWAKDALCRLEE
                     EKGRSLGLSAFTLMDDLVDGTKPGAGNLIFLPYLLGERSPRWNINARGAFIGLSSTHR
                     KPDMLRAVMEGVTFNLKIILDILEKDGKFDKIRLIGGGAKGRNWKQIVADIFGKTVTI
                     PEYLEEATSMGAAIIGAVGTGEMTLKEASTFVKDVQFISPRMEVHEKYLRLFEVFDKA
                     YMSLLEVYEDLAEM"
BASE COUNT          780 a         1012 c          852 g          980 t
ORIGIN      
        1 ctctatgctg gcggtgatgg ccgctatttc ggctcccagc tcccgggcct ttctacagtt
       61 aagaaccacc gaaggagtct cgcccgagcc cgtacccact atgagcagat caccctcgcc
      121 tatggaggga gtggagatct cccccaccac gtggaccttc agaccgaggt gcatcaatct
      181 catggcgaag gcctttatgg ccaatcccga tctgcccatg gcgaagagaa agatcctttc
      241 ggccccgagt atttttagag ctagtgattc aactgattcg tccgttactc cagagaggat
      301 cgagctaatc tcatctagca ccttttctaa ggtttctttg aaattcatat cgatcccgcc
      361 attctctttt tcatggtggc cgccgcttcc acgggatttt cggccctcga tattgaccgt
      421 ccggctatta cgacatccgg tctcagcgaa agtagaaccg ggagattatc cagggagatc
      481 ccacctgcaa cggctatcat ggtgtgtgga aggatccttc tgagtgtgcc catcttttcg
      541 gcgtaaccgg tcaaatgcga gtcttcaccg acatcgctca gatcggccga agtgtgaaga
      601 cagacgtaat ccggcgagaa cggagccacc ttcgaggcga actcctcgat cgattcgatt
      661 gcgagtgtgt cgagcattat ctgtccaccg aactcttcgg cggccttgac ggtctcctgg
      721 atagtcttga cgggtgcaaa ggccagaact gtaacgatat ccgcgccgct ttggaaagcc
      781 atcgcggctt ctagatatcc accatcgacg atcttggcgt ccgccagcac tttgtgcccg
      841 gggaaagccg ctttcgtttc tctgaccagt tccatcccgt gtgccaaaag cgccggggtg
      901 cctacctcta tgatatctac gtgagaagcg actttaatgg ccagagtgaa gaaatcgctc
      961 cttatatgac tgtcgtttgc cagctgtaaa agcatgactc agtaagttac cccggcgacc
     1021 agtatcacgc tcgagtaagg gatctgctcg cccgttctta ttactccctt ggactgtgtg
     1081 ttggtaagtt ctttgaactt cgtgtgtgga acgaattcga tctctatacc ttcgggcaat
     1141 ctcttgatgg tctcttctag agtcttggga ctgacggttt tcatctcttg cgagaagatc
     1201 actttctcta tttccagttc ctccagaaca ggctctaaaa cattgaagat accgggtttg
     1261 ccgccgatta tactcacatc gatccggtat ctgtccgaag ctatgggaag accggaatcg
     1321 gagataacca tgagatctgt gtgtcccatc gaacctatta aattgcagat ttccttattc
     1381 aatattccat ttttcttcat tatttgtctc ccttcatttt ccggttgaaa gatggatccg
     1441 gaatttgtag tgcctcgctt cgtaccagca ctgtacaagc tcgaccggct cgttgtttat
     1501 cgtcgaaccc agcctttcgt aatagagtat cggggcaccg ttctccactt tcaaaagttc
     1561 cgcaatcttg ccttcggcca gccttccctc tattattgcg tctccatcga cgacgggcga
     1621 gcccttcgac ctcatgagtt cgtacagaga tccttcgaag ttttcctcgc ctgtcaatct
     1681 gctcccgggg gtgagataag aaatgaaaag cgcaagagga aagtaagatt cattgcccct
     1741 caatctggtt acctgaagca gctcctcgcc ctcggtaacg ccaaggcgtt tggccagttc
     1801 cgaatcggcc agaacctttc gaacatccac aacctgcgtt atgggcacat ggttctcgag
     1861 cctcatctgc tcagtaaaac ttcttatagt ggtaagatgc tcctgcgaag cgttattggt
     1921 aactatcgtg ccccttccgc gtcttctggc tatctgaccg ctgctttcca gagcctggag
     1981 cgctctcctg atagttgccc tgcttacatc gaagactttc atcatcgctt cttcggtcgg
     2041 aagtttcctg ccgacaacgc ccggctcctt catgtagtcg gagattatcc tgtatatctg
     2101 gtaataaagc ggaaccgggc tggctctatc cagtactatt cttctatccg agttaaacag
     2161 catgatgaaa tcccttcttt ctttcggtgt cgcttcaaga gatgaagatc cgatatatca
     2221 aacttgaatc acatctccgc aaggtcctca tagacttcga gaagagacat gtatgcttta
     2281 tcgaacactt caaagagcct cagatatttc tcgtgaactt ccattctcgg cgagatgaac
     2341 tgcacatcct tgacgaaagt ggatgcttct ttcagcgtca tttctccagt gccgacggct
     2401 cctatgatcg ctgcacccat tgaagttgct tcttccaaat actccgggat agtaactgtt
     2461 ttaccaaaga tatcggcgac gatctgtttc cagttccgtc ctttggctcc accgcctatc
     2521 aaccttattt tatcaaactt tccgtccttt tccagtatat ccaggattat cttcaggttg
     2581 aaagtaaccc cttccatgac ggccctcagc atatcgggtt tcctgtgtgt tgaagaaagc
     2641 cctatgaagg ctcccctggc gtttatgttc cagcgagggc tacgttcgcc gaggaggtat
     2701 ggcaaaaaga tcaggtttcc ggctcccggt ttagtaccgt ccaccagatc gtccatcaaa
     2761 gtgaaggcgc tgaggccgag agaccttccc ttctcttctt cgagcctgca gagagcgtcc
     2821 ttggcccagt ggtaagatgc acctccggcc tgcattgtgc cgcagggaca gtagtatccc
     2881 tctatcgggt aggcgaaatt gaaggttctc gacttctcgt cgaaaaccgg ttctcttgag
     2941 caagtgctta tccagctcga tgagccgaga tagagatagg cctcgtcggg acttacaaca
     3001 ccggccccca gacaggcgca ggctccgtcg ccgccacccc ttataaccag cgtcttttcg
     3061 gagagcccca gttccctcgc cacgacgggc aaaaccgtag atactacaga ggtcgagggg
     3121 aagacttcgg gaagcttctc aaggtcaagt cccagcgtgt ctgcaagtcg ttccgaccat
     3181 ttccagttct ttatatcgaa cagcagggtc atcgaagcgt ccgtcggatc ggtaccgaac
     3241 tcgccggtga atctggagat aatgtagtcc ttggcctgga gaaacttatg tgccctcctg
     3301 tacgtttccg gttcgttgtc tctgatccag gcgatcttcg gaccggaata agtcggtgtg
     3361 attcgtgagc cggtcaatct gtacacagta tcgttgccca acttttccaa cctggcggcc
     3421 tgttctacac tgcgttgatc ggcccagatt attgcccttc tcagggtttt tccgttccga
     3481 tcgacgggaa tagctcccat catctggccg ctgaagctga ccgctttgat ctcctgcgta
     3541 tcgacaccgg ctttcgataa aagctccgat gtcgttatct ttactgactc ccaccactcc
     3601 tcggggtcct gttcaacctg gttg
//
LOCUS       DAOOLY010000252         3601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31308_length_3601_cov_3.749013, whole genome shotgun sequence.
ACCESSION   DAOOLY010000252 DAOOLY010000000
VERSION     DAOOLY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31308_length_3601_cov_3.749013"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..2085)
                     /locus_tag="PLD15_10495"
     CDS             complement(70..2085)
                     /locus_tag="PLD15_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB64382.1"
                     /translation="MRKWFFWALLMIVVMVVFAIRLLPDILNKPPVLNIPDLIIDEGA
                     VITIDLHDYSSDEKKSLLVFQKISGPGEIKGNLYTFSPTFQQSGKHSVSIIAKDQSGQ
                     IAEEVFTIEVREINRPPVFALPSLTLGKGEMVSINLDNYAKDLDLDTLTYSVESGGGQ
                     IIGSSYTYRPSFSDSREETVSIKVSDGKGGVVTSVLKLSERDFIASIDQQSQRATDTT
                     AQGTVSTVADTQIQTPITSNNPPVISIPDQRMNQGETLAIDLKGFITDKDNDAITIRK
                     ISGPGILERTLFSYSSPTNDSGQKLIEIEVSDGKTAVRSSFKIDVVAQNRSPIAIDIP
                     QNSVVQGQTFSITLNNYFSDPDGDALSYQILSGPGNIQNGLFTFQSQTTGSFSTSIRA
                     SDGKGGTIDRTLTINVQASNRPPVISITPRRIVEGEVLTIDLASNSSDPDNDRLSYTL
                     ASGPGTVQGNIYTLRTDFNSAGNYTVVIRADDGKGGSTSGSFAVEVRDVNRPPSLELS
                     GITVPEGSTYSIDLTKASSDPDGDTLTYSIIQGIGRIENNNYIVEPNYDDYGFYTVVI
                     SASDGKGGQVSSNFDLLVTDTNRAPQFTIPDQTTMSTRTLRLDLREFSSDPDGDYLRY
                     ELIYGPGLLTGSIYSFIPEGLGTSTVMLKASDLKGGETTTTFTITVR"
     gene            2439..>3601
                     /locus_tag="PLD15_10500"
     CDS             2439..>3601
                     /locus_tag="PLD15_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPB64383.1"
                     /translation="MPVTITINGKDYQVKENQSILEACRDAGINIPTLCYHPSLSVVG
                     SCRVCVVEVEGARNLSPACATQVSDGMKIKTSSERVNKAVRFNLGLLLSNHPNECMTC
                     DVNGRCEFQDLIYQYDVKDIFPKTLRNVPYDISSPSLFRDMNKCIDCGRCVRACDELQ
                     GLSILSPVNRGYHVLPLPALGAPIAATDCINCGQCSVACPVGAITERMEFRDVITALR
                     KHDKVLVAQTAPSVRVALSEEFGELPGSISTDKMVAALRRLGFDYVFDTNFTADLTII
                     EEGSELLERVKEGGKFPMFTSCCPGWVNMMEKLYPEFTQNLSSAKSPQEMMSALIKTY
                     FAEKIGKKPEDIYLVSIMPCTAKKDEKSRETLSVKGMQATDAVIVPRELAKLI"
BASE COUNT          930 a          828 c          823 g         1020 t
ORIGIN      
        1 aagaagttat ggtatgtttt tcatttgcac gatcgctttt tttgatagat gtttcatttc
       61 gatcctcaat tacctgaccg taattgtaaa tgtggtagtc gtttctccac ctttcaggtc
      121 actggctttc aacattaccg tgctggtgcc gagtccctcg ggaataaagg agtagatact
      181 tccagtcaga agacccggtc cataaatcag ttcgtatcta agataatctc cgtccggatc
      241 gctgctgaac tccctcagat caagccttaa tgttctggtg gacattgtcg tctgatcggg
      301 tatcgtaaat tgtggtgccc tgttagtgtc ggttaccaaa agatcgaaat tagacgaaac
      361 ctggcctccc ttaccgtctg aggcacttat aaccacagtg tagaaaccat aatcgtcgta
      421 attgggttcc acaatgtaat tgttgttctc gatcctcccg ataccctgaa ttatcgagta
      481 agtgagagta tctccgtcgg ggtccgaaga ggcttttgta agatctatag agtatgtaga
      541 accttccgga acggttatac ccgaaagctc cagtgatggt ggtctgttga cgtctctgac
      601 ctctacggca aaggagccac ttgtactgcc tccctttcca tcgtcggctc tgatgacaac
      661 cgtatagtta ccagccgagt tgaagtccgt cctaagtgtg tagatgttgc cctgaacggt
      721 tcccggtccc gatgcaaggg tatagctcaa cctgtcgttg tcgggatcgg aggaattgga
      781 cgccaggtcg atcgtcagga cttctccctc gacgattctt ctaggagtta tcgaaataac
      841 gggaggtctg ttggatgcct gaacatttat tgttaaagtt ctgtcaatgg ttccaccttt
      901 gccatcggaa gctctgattg aagttgaaaa ggaacctgtg gtttgcgact ggaaagtgaa
      961 aaggccattt tgaatgtttc ccggtccact caggatctga tatgataggg cgtcgccatc
     1021 ggggtcgctg aaatagttgt tgagggtaat tgaaaaagtc tggccctgaa ctacagaatt
     1081 ctggggaata tctatggcaa tgggtgatct gttctgagcc accacatcaa ttttgaaact
     1141 tgatcgcaca gcagtcttac cgtcggagac ttcaatttct atcaacttct gaccactatc
     1201 gtttgtggga ctcgaatatg agaaaagcgt tcgctccaaa attccaggtc ccgatatttt
     1261 ccttatcgtg atcgcatcgt tgtctttgtc ggttataaaa cctttcagat ctatcgcaag
     1321 agtttcaccc tgattcattc tctgatctgg aatagatatc accggtggat tgttggaagt
     1381 tatcggtgtt tgtatctgcg tatccgctac ggttgaaacc gttccctgag ccgttgtatc
     1441 ggtagcacgc tgcgattgct gatcgatgga agctatgaaa tccctttcag aaagcttgag
     1501 taccgaggta acaaccccac cctttccgtc ggacaccttt atacttacag tttcttctct
     1561 ggaatctgaa aaggacggtc tgtaagtata actacttcct attatctgac caccacccga
     1621 ttctaccgag taagtcagag tatcgagatc tagatctttc gcataattat ccaggtttat
     1681 ggaaaccatc tcgccctttc ctaaagttaa cgatggcaag gcaaagacag gcggacggtt
     1741 tatttccctg acttctatag taaaaacttc ctctgcaatc tgaccgcttt gatccttggc
     1801 aattatactc acagaatgtt tacccgattg ctgaaaggtt ggagaaaacg tgtatagatt
     1861 accttttatc tcaccgggtc cagagatttt ctggaagacg agtaagctct ttttctcatc
     1921 actcgaataa tcgtgcaaat cgatggttat tacggcgcct tcatcgatta tcaaatcggg
     1981 aatattcaac accggcggct tgttcagaat atctggcagc agtctaatag cgaagaccac
     2041 catcactacg atcatcagaa gagcccagaa gaaccatttt ctcataccgc acaacctcca
     2101 tagaagagaa aactgtacaa caacattata atcttctctg gcatcacgcc aaagagaagt
     2161 ctctattatt atgacactaa atcgaccgta gattgttcgt ccggttcaac catttcgctc
     2221 cggcactttc aataaaaagt ttacctgtga acgctcactg tgtacgatac tgtttttttc
     2281 aaggcttcgg tctggccttt cgtttatttt gatataataa ttttaacctt ttatctggca
     2341 tattgtgcac cgcttgtgct agaatcaatt tgatagaaaa atcacaatct ttttccgttc
     2401 tatatatctg ttgaagattt tctaaggagg tgctataggt gcccgttact atcaccatca
     2461 acggcaagga ttatcaggta aaggaaaatc agagtattct cgaagcctgc cgtgacgccg
     2521 ggataaacat cccaacgctt tgctaccacc cgtccctgtc ggtcgtgggt tcgtgcaggg
     2581 tctgtgttgt tgaagtcgag ggagcgagaa atctttcgcc ggcctgcgcg acacaggtgt
     2641 cggatggaat gaaaatcaaa accagctccg agagagtgaa caaagctgta aggtttaatc
     2701 ttggtcttct tctttccaat cacccgaacg agtgtatgac ctgcgatgtc aacggcagat
     2761 gtgaatttca ggacctaata taccaatacg atgtgaagga tatattcccc aagactttga
     2821 ggaatgttcc ttacgacatt tccagtccat cactcttcag ggatatgaac aagtgtatag
     2881 attgtggaag atgcgtcagg gcttgcgatg agcttcaggg gttgtcgata ctatcgccag
     2941 tcaacagggg ctaccacgtg cttccactcc cggctctagg agcacctatc gccgctacgg
     3001 attgtattaa ttgtggacag tgttccgttg cctgccctgt cggtgcgata accgaaagaa
     3061 tggaattcag agatgtcatc accgctcttc gcaaacacga caaggttcta gttgcccaga
     3121 cggccccgtc tgtacgtgtg gcactttccg aagagtttgg tgagctaccc ggatcgataa
     3181 gcaccgacaa aatggtcgca gcgctgagaa gactgggttt tgattatgtc ttcgatacca
     3241 attttacggc cgacctcact ataattgaag agggttcgga actccttgaa agggtgaaag
     3301 agggcggtaa attcccaatg ttcacctcct gttgccctgg ttgggtcaac atgatggaga
     3361 agctctatcc cgaattcacc cagaatcttt catcggctaa gtctccacag gaaatgatga
     3421 gcgcccttat caagacgtat tttgctgaga agatcggcaa gaaacccgaa gatatctatc
     3481 tggtctcgat aatgccctgt accgcaaaga aggatgaaaa gagcagagaa actctttcgg
     3541 taaagggtat gcaggcaacc gacgccgtta ttgtacccag agagctggcc aagttgatca
     3601 a
//
LOCUS       DAOOLY010000253         3597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31357_length_3597_cov_5.946923, whole genome shotgun sequence.
ACCESSION   DAOOLY010000253 DAOOLY010000000
VERSION     DAOOLY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31357_length_3597_cov_5.946923"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLD15_10505"
     CDS             <1..363
                     /locus_tag="PLD15_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPB64384.1"
                     /translation="NIINGARESGVSKLVAIANAGILQLSPRELRLDSPNYPQYLKKT
                     SREYLDAFEILKTSELDWTVVCPPFMSFAEGNQSYRVAADFLPESGERISVQDVAEFA
                     YNLLFTSEYSQKRVGIAY"
     gene            373..984
                     /locus_tag="PLD15_10510"
     CDS             373..984
                     /locus_tag="PLD15_10510"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HPB64385.1"
                     /translation="MRFSSSDRQTNVNRLEALTDGVFSIAITLLVLTIRIPEPGEIVS
                     QKELIKYILSLDYELFSYCISFFVVGSLWISGNKRMKLLRKTDDLHLWLSLTSLLFIT
                     LIPLTTSLLSDYGDFEFVELLFHGNILMLEIITLIEWEYLIRKTDLVQEEVLSNLDIR
                     NTRKKNLFSVSVPILGMLISILTPAWSNLVYLLLFFRSLIIRY"
     gene            1041..1391
                     /locus_tag="PLD15_10515"
     CDS             1041..1391
                     /locus_tag="PLD15_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HPB64386.1"
                     /translation="MVLTIAKFIISILTATTGLYIIIRPQSYARLAGFSAGGDRGRVE
                     IRTIMGGAFLGLGLAPVILFNYPIALTFVGVVYIFVALTRLVSLFLDRSFEFSSIMFL
                     IVEASFGLIMVIPS"
     gene            1475..2188
                     /locus_tag="PLD15_10520"
     CDS             1475..2188
                     /locus_tag="PLD15_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPB64387.1"
                     /translation="MKEEEMKLRILEEWTGYMGIDLTVLERESIMFVPEKKKESGRIS
                     VFSFENKIILIFDPALENIVRPFEELSEFSVEKIPALSGELLKRGRKTSIKYLNPSNF
                     VPFDPPDEFIVRRLNLGDRTAFYNFRSHCTREDLVEGYVELNHVAVFGAFYEREIVAV
                     SSIIKWGKIADIGIITIPDFRELGLGKALGSKSSEWGILSKNLVQYRHDVLNYGSLKV
                     SRALGFKEYMVQQDFYPAR"
     gene            complement(2204..2818)
                     /locus_tag="PLD15_10525"
     CDS             complement(2204..2818)
                     /locus_tag="PLD15_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64388.1"
                     /translation="MKRREWLEKYKREMLDRPARSAASPEKLQDYLLRMMGFVEESSI
                     VADFGCGVCNLWPLLRDMGMRIVGVSCNRKEQQQKGIRIMNIEMTDFNLLGIFEGFVA
                     VDLLQRIPPELWSKILLKISRSIKTDGVGLIVTELSEEKSARSFFSDLKGDGLPVVVG
                     ENLEDGEYSFRPLKSWYDQWLKNAGLSAFREEVIDSVVYSIVRK"
     gene            complement(2935..>3597)
                     /locus_tag="PLD15_10530"
     CDS             complement(2935..>3597)
                     /locus_tag="PLD15_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64389.1"
                     /translation="QTRMGSIQNAVDNLRNLVEEPYRLLKSKEFFTVSVGFDEVIVKS
                     LFTFSEYKEGSDVFLVLNDEAGSTRTLELDREDGVSFIELSISPFGIYSGQILVREGS
                     REIMSDRFQIPPENYRIQNFEISGRAWQQDQNDLLYSFHLITTSFIQNSQLKIAKASV
                     RVMNQGEVIDVLDMPFQDGQTALTTTNYTAGRDSNYEFFADVTYGFGGSVSRKIEVKY
                     NF"
BASE COUNT          934 a          831 c          808 g         1024 t
ORIGIN      
        1 aacataatta acggcgccag agaatcggga gtctccaagt tagtcgctat agccaacgcc
       61 ggtattcttc aactatcgcc gcgagagcta agactggact cgcccaacta tcctcagtac
      121 ctgaaaaaaa catcgcggga gtatctggat gctttcgaaa tactcaaaac ttccgaactg
      181 gactggacgg ttgtgtgtcc tccctttatg tcgtttgccg agggtaatca gagttaccgg
      241 gtcgcggccg attttcttcc ggaaagcggc gagcgcattt cggttcagga tgtggcggaa
      301 tttgcctata acctgttgtt cacttcggaa tattcacaga agagagttgg catagcttat
      361 taggagcgag atttgaggtt ttcatcttca gaccggcaga ccaatgtcaa caggctggag
      421 gcgctgaccg acggagtctt ttcaatagcc ataactctcc ttgtactcac aataaggatt
      481 ccggaaccgg gggagatagt ctctcagaaa gagctcatca aatatatcct cagcctcgac
      541 tacgagctgt tcagttactg catctccttt ttcgtggttg gatcgctctg gatttcaggg
      601 aacaaacgaa tgaagttgtt gaggaaaacc gatgatcttc atctgtggtt aagcctcacg
      661 agccttctat ttataactct cattccgttg accacgtctc tgttaagcga ttacggggat
      721 ttcgaatttg tcgagcttct cttccacgga aatattttga tgcttgaaat cataacctta
      781 attgagtggg agtacctgat aagaaagacc gatctcgttc aagaggaggt tctaagcaat
      841 ctggatatcc gaaacaccag aaagaagaac ctcttctccg tctccgttcc tatacttgga
      901 atgttgatca gtatcttgac tcccgcatgg agcaatctgg tctatttgct gctttttttc
      961 agatctctta taatccgcta ttgatttgct atcgaaacat tgtactgcta gaataatata
     1021 gaattccggg gggcgtgaat atggtgctca ctatagccaa gtttattatc tctattctca
     1081 cggcaacaac gggtttatac ataataatca gacctcaatc ctacgccagg ctggccggat
     1141 tttctgccgg tggagatagg ggaagggtag agatcagaac cattatggga ggagctttct
     1201 tgggtcttgg tctggctccc gttattcttt tcaattatcc gatcgcactg acgttcgttg
     1261 gggtggtcta tatctttgtc gccttaacgc gtctggtatc gctttttctg gataggtcct
     1321 ttgagttttc cagcatcatg tttctaattg ttgaagcaag cttcggattg atcatggtga
     1381 ttccgtctta gtgccttctc gatctttctg aattcagttg tgataaagac actatacgtt
     1441 tgttttctgt cccgaaagaa tggaggtact caatttgaaa gaggaagaaa tgaaactcag
     1501 aatactcgaa gaatggaccg ggtacatggg tatcgatttg acggttctgg aaagagaatc
     1561 gattatgttc gttccggaaa agaaaaagga gagcggtcgg ataagcgttt tctcttttga
     1621 aaataaaatt atcctgattt tcgatcccgc actcgaaaat atcgtgagac ccttcgaaga
     1681 actgtccgaa ttcagcgtcg agaagatccc tgctctatcc ggagagctgc tgaagagggg
     1741 acggaaaact tctataaagt atttaaatcc ctccaacttc gtacctttcg acccgcccga
     1801 tgaattcatc gttcgcagat tgaatctggg cgacaggacg gcattttata atttcagaag
     1861 tcattgcacg cgcgaagatc tggtggaagg atatgtagag ttaaatcatg tcgcggtttt
     1921 cggagcgttt tacgaaagag aaatagttgc cgtttccagc atcataaaat ggggaaagat
     1981 cgccgacatt ggcatcataa ccattcccga cttcagggaa ctcggtcttg gcaaggctct
     2041 gggctctaaa tccagcgagt gggggattct cagcaaaaac ctggttcaat acaggcatga
     2101 cgtacttaac tacggctcac tcaaagtttc tcgtgcgctt ggattcaaag agtacatggt
     2161 ccagcaggat ttttatccgg cacgttgaat ccggagaaat gcgttacttt ctcacgatcg
     2221 aatagactac cgaatcgatg acttcctctc tgaaagcgga caatccggca ttttttagcc
     2281 actgatcgta ccagctcttg agcggtctga aactgtactc gccgtcttcc agattttctc
     2341 caactaccac ggggagacca tcgcctttga ggtccgagaa aaacgaacgg gccgatttct
     2401 cttcgctcaa ctcggtaact attagaccga cgccatcggt tttgatagaa cgtgagatct
     2461 tcaatagaat tttcgaccag agctcgggcg gaatacgctg tagaagatcg accgctacaa
     2521 aaccttcaaa tatgccaagc aagttgaaat cggtcatctc tatgttcatt attcggattc
     2581 ccttctgctg ctgctccttt ctgttgcagg agactcccac tattcgcata cccatgtccc
     2641 gcaaaagcgg ccagagattg cagacgccac atccaaagtc cgctacgata gaggattcct
     2701 caacgaatcc catcattcga agcaggtaat cctggagctt ttcgggactg gccgcacttc
     2761 gcgccggtcg gtcgagcatc tcccttttgt atttttctag ccattcccgt ctcttcaata
     2821 tactcacctt ccatggaaat tttatcggag acgcacctat aaacaaacaa aaagaacgac
     2881 tctagttttt ctgatcaaaa ctgatcaaaa gagtcgttct tacataaaac cggcttaaaa
     2941 attgtacttg acttctattt tcctgctaac tgagcctccg aagccataag taacgtctgc
     3001 gaagaattcg taattgctgt ctctgccagc cgtgtaattt gtagttgtga gtgccgtttg
     3061 cccatcctgg aagggcatat ctagtacgtc tatcacttca ccctgattca taacccgtac
     3121 tgaagcttta gcgattttga gctgactgtt ttgaataaaa gaggttgtta tcagatgaaa
     3181 agagtaaagc aaatcgttct ggtcctgctg ccaggctctg cctgagattt caaagttctg
     3241 tatacggtaa ttctcgggag gtatttgaaa acggtcgctc attatttctc gggatccttc
     3301 ccgtaccaag atctgcccgc tgtaaattcc aaacggagaa atggatagtt cgataaagga
     3361 aactccatct tccctatcga gttccagggt tcttgttgaa ccggcttcgt cgttcaagac
     3421 gagaaatacg tcgcttcctt ctttatactc ggaaaatgtg aaaagcgact tcacgatcac
     3481 ttcgtcgaac ccgacgctta cagtaaagaa ctctttcgac ttcaataatc tgtacggctc
     3541 ttctacaaga ttgcggagat tgtcaactgc attctggata ctccccatgc gcgtctg
//
LOCUS       DAOOLY010000254         3597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31361_length_3597_cov_4.671937, whole genome shotgun sequence.
ACCESSION   DAOOLY010000254 DAOOLY010000000
VERSION     DAOOLY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31361_length_3597_cov_4.671937"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /gene="thyX"
                     /locus_tag="PLD15_10535"
     CDS             complement(<1..579)
                     /gene="thyX"
                     /locus_tag="PLD15_10535"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPB64390.1"
                     /translation="MEIKVLDKGFVELVEILGDDYSVVQAARTSYGKGLTTPDRDRKL
                     INYLMERGHHSPFEHVVFKFHIKLPIFVMRQLVRHRIASINERSGRYTQFEEEWFLPD
                     EIRVPDPEDRQKSIPFDDKELNRKALEIMRESMDNSFKAYEELISMGVAREMARIILP
                     ISMYTEAYWTINLRSMMNFLNQRADSHAQYEIQ"
     gene            complement(557..1714)
                     /locus_tag="PLD15_10540"
     CDS             complement(557..1714)
                     /locus_tag="PLD15_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB64391.1"
                     /translation="MIFLCTAVFAFEHAGVILSKDYLEQAQSLDTQEIDSRALLTIAV
                     GLKFKETIQPNYKTWFVNLKSSLAGVGLLEDIARSLAVVDSMVSTSTVSALNMLGTFS
                     DPGPLLEAVSLRLHYYDWLETSDPRSSKIINTLAVDMLGRDPSQYLPHKVMLDLYSSS
                     SYMRLEPLNEIRKGIFENGLGSLLGPDLVESEFAAGMFESVIEDFHSLALNDPVSIYY
                     AATAYLRTGKGSEGRAMLESIELRKLPPRLASSAAMALGDALFHEGSMLESIELFQQS
                     IRFWPENSRAVRNLGMAYYETQNREYYDLARFYLQLSGYEAFDESVSAALKELRRRVI
                     FEMVLVTVVPIGVIVVLALFLLEYISKRRRASQIEKALRNDGGFNGDKGSR"
     gene            complement(1735..2529)
                     /locus_tag="PLD15_10545"
     CDS             complement(1735..2529)
                     /locus_tag="PLD15_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPB64392.1"
                     /translation="MILKSKPIFSPRPWGDRLLNELYKVDTQDPVGEVWLLSDFQGMR
                     TELCEGERCIYPVDLTEPLFNKDLPRFPLLVKYICANDWLSVQVHPDDELAKSLEGDP
                     WGKNECWYFLQKGRIAAGITRQEKPLEDLRNGDLRILTPERGDLACLQAGTVHSLGPG
                     SKVIEIQQASDMTYRIYDWGRGRELHLDKARVATKAAASAIVIEEMRKFDWKYFSIEL
                     TKKVEGSGIVITLSEVPELHVLIDDREEFSEEALLVRPGSFWSELL"
     gene            complement(2513..>3597)
                     /gene="trmFO"
                     /locus_tag="PLD15_10550"
     CDS             complement(2513..>3597)
                     /gene="trmFO"
                     /locus_tag="PLD15_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPB64393.1"
                     /translation="IAHRHRVPAGKALAVDRVGFSREVTDLIESNPLIEIRREEVSSL
                     DLQRGVNVVCTGPLTSPKLESFLGQLFGEHLFFFDAVSPIIEAGSIDFERGFFADRYS
                     EEGDYLNCPLDGEEYGDFWRELVEAELAKMENFSDRYLFERCQPVEEIARSGFDALRF
                     GPMKPVGLIDPSSGKEPFAVVQLRKENSSGSLMGLVGFQTRLQWGEQKRVFSMIPALR
                     NSQFVRYGVMHRNTYLNSPILLDKNLGSKEYGGLFFAGQITGLEGYVEAIVSGRVAAL
                     NVERYLRGVGALPIPVTTMIGGLIEHVTVSGRSPLKPVYSNFGLLPPVAGRGKRDRNR
                     EKVSRARDDMDFFLKEWRSDRNDTEE"
BASE COUNT          830 a          980 c          846 g          941 t
ORIGIN      
        1 ctggatttcg tactgcgcgt gtgaatcggc cctctggttc aggaaattca tcatcgatct
       61 cagatttatc gtccagtatg cctctgtata catggatata gggagtatga tccgggccat
      121 ctctctggcc acacccatcg agatcagctc ttcgtaagcc ttgaaagaat tatccatcga
      181 ttctctcatt atctcgaggg ccttccggtt gagttcttta tcgtcgaagg gtatggattt
      241 ctgtctgtct tccgggtctg ggacccttat ctcatcggga aggaaccatt cctcttcgaa
      301 ctgggtgtat ctcccgctcc tttcgttgat cgaggctatt ctgtgcctca ccagctgcct
      361 catgacgaat atcggcaact ttatgtggaa cttgaaaacc acgtgctcga aaggagagtg
      421 gtgaccgcgt tccatgagat aattgatcaa tttcctgtcc ctgtcggggg ttgtcaatcc
      481 ttttccgtaa ctcgttcttg ccgcctgaac gacgctgtaa tcatcaccca ggatctctac
      541 gagttctaca aatcctttat ctagaacctt tatctccatt gaaacctcca tcgtttctca
      601 aagccttttc tatctgtgaa gctcttcttc tcttactaat gtattctagc agaaataagg
      661 caaggacgac tattacaccg atcggaacta ctgtaacgag aaccatttcg aagatgacgc
      721 ggcgcctcaa ctctttgaga gcggcagaaa ccgattcgtc gaaagcctcg taaccgctca
      781 gttgcaggta aaaccttgca aggtcgtaat actctctgtt ttgcgtttcg tagtaagcca
      841 ttccgaggtt cctcaccgcc ctggaattct cgggccagaa gcgtatcgat tgctgaaata
      901 gctcgatcga ttccagcatg cttccctcgt gaaatagtgc gtcccccagc gccatcgcgg
      961 cactggatgc caatctgggt ggaagctttc tcagttctat gctctccagc atcgccctcc
     1021 cttcactgcc ctttcccgtt ctcaggtaag cggtagccgc gtaatagatg gaaactggat
     1081 cgttgagggc tagagagtgg aaatcctcta tgaccgattc gaacataccg gccgcaaatt
     1141 cgctctcaac caagtccggt cccagaagag atccgaggcc gttttcgaat attcctttcc
     1201 tgatttcatt cagtggttca agtctcatgt aactggagct ggaatagaga tccagcatca
     1261 ctttgtgggg gaggtattga ctgggatccc ttccgagcat atcaacggcc agcgtgttga
     1321 ttattttcga tgaacgcgga tcggaggtct ctagccagtc gtaataatgt agcctcaacg
     1381 agactgcctc caaaagaggc cccgggtcgc taaatgtccc gagcatattt agagccgaaa
     1441 ccgtcgaggt ggaaaccata gaatcgacca cggccaggga gcgggcgata tcttccagaa
     1501 gaccgacacc cgctagtgaa gacttcaggt tgacgaacca tgtcttgtag ttgggctgta
     1561 tagtttcctt gaatttcaga ccgaccgcaa tcgtcagaag ggccctggaa tcgatctctt
     1621 gagtgtctag agactgtgcc tgttcaagat agtccttcga aaggataacg ccggcatgtt
     1681 caaaggcgaa aaccgcggta cacaaaaaaa tcactgcgaa gatcgtcgag agtttcaaag
     1741 caattcactc cagaaacttc ccgggcgaac gagaagagct tcttcggaga actcctcccg
     1801 gtcgtctatc aacacgtgga gttcgggcac ctctgagagt gtaatgacta ttccgctgcc
     1861 ttcgaccttt ttggtgagtt caatgctgaa atacttccag tcgaatttcc tcatctcttc
     1921 aatcacgatg gcggaagcag cagcttttgt ggcgactctg gctttgtcca gatggagttc
     1981 tctccctctt ccccagtcgt atattctgta ggtcatatcg ctggcctgtt gaatctctat
     2041 cactttcgac cccggtccaa gcgaatggac cgtaccggcc tgcaaacatg cgagatcccc
     2101 cctttcgggc gtcagtatcc tgagatcccc gtttcttaga tcttcgagcg gcttttcctg
     2161 cctggttatt ccagctgcga ttcttccctt ctgaaggaaa taccagcatt cattcttgcc
     2221 ccagggatcg ccttccagag attttgccag ttcatcgtcg ggatggacct gaacggagag
     2281 ccagtcgttg gcacatatat atttcaccag cagtggaaac ctcggaagat ctttgttgaa
     2341 aagaggttca gtcagatcta ccggatagat acacctctct ccctcgcaca gctcagtcct
     2401 cataccctga aagtcggaca ggagccagac ctctcccacc gggtcttgtg tatcaacctt
     2461 gtatagctcg ttcaacagac gatctcccca cggtctcgga gagaagatcg gtttactctt
     2521 cagtatcatt tctatcagac ctccattcct tcagaaagaa gtccatgtca tctctcgctc
     2581 tggagacctt ttccctgttt ctgtcacgct ttccgcgacc ggcaacaggc ggaaggagac
     2641 cgaagttgga ataaacgggt ttgagtggag atcttccgga aaccgtaaca tgctctatta
     2701 aaccgccgat catcgtcgtc accggtattg gaagcgcccc gactcctctt aggtaccgtt
     2761 ccacgttcag tgccgcaact ctaccagaga cgatcgcctc tacgtagcct tcgagaccgg
     2821 ttatctgacc ggcgaagaac agtcccccgt actcttttga accgaggttt ttatccagca
     2881 ggatcggcga gttgaggtaa gtgtttctgt gcatgactcc gtatctaacg aattgggagt
     2941 ttctcagggc aggaatcatg gaaaagactc tcttctgttc tccccactgc aacctggtct
     3001 gaaatccgac gagacccatg agcgaaccgc tggagttctc ctttctgagc tgaacgacgg
     3061 caaagggttc ctttccactg gagggatcga tcagacccac aggcttcatc gggccgaatc
     3121 tgagcgcgtc gaagccgctt ctggctatct cttcaacggg ctgacagcgc tcgaagagat
     3181 atctgtccga gaaattctcc atcttcgcga gttctgcctc taccaactct ctccagaaat
     3241 cgccgtactc ttcgccatcg agcggacagt tgagatagtc tccttcctcg gagtacctgt
     3301 cggcgaaaaa acctctttcg aaatcgatcg acccggcctc gattatgggc gagacggcat
     3361 cgaagaagaa gaggtgctct ccaaacaact gtcccagaaa gctctcaagc ttcggtgagg
     3421 ttagcggacc ggtgcatact acgttgactc ccctttgaag atccagagag gagacctcct
     3481 ctctacgtat ctcgataagg ggattggatt cgataagatc tgtaacttct cgtgagaacc
     3541 caaccctgtc aactgcaaga gcctttcccg cagggactct gtgtcggtga gctatct
//
LOCUS       DAOOLY010000255         3584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31517_length_3584_cov_4.224426, whole genome shotgun sequence.
ACCESSION   DAOOLY010000255 DAOOLY010000000
VERSION     DAOOLY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31517_length_3584_cov_4.224426"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLD15_10555"
     CDS             complement(<1..568)
                     /locus_tag="PLD15_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPB64394.1"
                     /translation="MTDFIEPSENRILVIGGYLQDIEITGSGHTASVISRPGGSAYNV
                     AMCLSSMGLDVLFLSCFGPGNRVGYAFDSLPVQSGCQRGIFLYRGTEVLAVQRPSRPA
                     VSGALRRLLEGEKFALAYTTLEIGAEPLNLLDAIDSRYRILDPSPGIELKNLSGFEKF
                     DYVLANDHTRVIACGSKLIIKAGAKGAIH"
     gene            complement(543..1175)
                     /gene="upp"
                     /locus_tag="PLD15_10560"
     CDS             complement(543..1175)
                     /gene="upp"
                     /locus_tag="PLD15_10560"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPB64395.1"
                     /translation="MFEQLTIVYHPLIEHKLTIMRDDQTGPKEFRELLKEITLLLTYE
                     ATRHIPTYEKEIRTPLVKMHGKMIEDKKVTVVPILRAGLGMVEGVLSLMPNASVGYIG
                     IYRDPDTIQPVEYYSKLPKIDDTTQIFVLDPMLATGVSSSWALKLVKKAGGRQISLMC
                     LIAAPEGVKFIQKNHPDVKVFTAALDERLNDHAYIIPGLGDAGDRLYRTK"
     gene            complement(1168..2457)
                     /gene="glyA"
                     /locus_tag="PLD15_10565"
     CDS             complement(1168..2457)
                     /gene="glyA"
                     /locus_tag="PLD15_10565"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPB64396.1"
                     /translation="MWEVLGKKDREVFDIIIKELKRQENGLELIASENFVSIAVMEAV
                     GSIMTNKYAEGYPAKRYYGGCRFVDEAENLARDRAKRLFNANFVNVQPHSGSQANMAA
                     YLAVTEPGVTIMGMSLSHGGHLTHGSPVNFSGKLFNVVAYGVDEDSEVIDYDSVRKLA
                     VENRPAVIVAGGSAYSRIIDFKKFREIADEVGAVLMVDMAHFAGLVAAGLYPNPLDFA
                     HIVTTTTHKTLRGPRGGMILTNDEEIGKSIDKTIFPGTQGGPLMHVIAAKAVSFGEAL
                     KDEFKVYQQNILNNAKKLSSSFEERGLRIVSGGTDTHLFLVDLNPKNVTGKAAEKALE
                     RAEITVNKNTIPKETKSPFVTSGIRIGTPAVTTRGMTEAEMPLIADLVVKVIENVTGD
                     KGEIDEIVVKEVGSQVKDLTDKFPLYADLVSRSELDV"
     gene            complement(2470..3386)
                     /gene="mtnA"
                     /locus_tag="PLD15_10570"
                     /pseudo
     CDS             complement(2470..3386)
                     /gene="mtnA"
                     /locus_tag="PLD15_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
BASE COUNT          757 a          992 c          847 g          988 t
ORIGIN      
        1 tgtgaatggc acctttggct ccggccttta ttatcagctt cgatccgcaa gctatcaccc
       61 gtgtatgatc gttcgccaga acataatcga acttttcaaa accagataga tttttaagct
      121 ctataccggg cgatggatcg agaattctgt atctggagtc gatagcgtca agaagattca
      181 gaggttcggc acctatctcg agtgtcgtgt acgcgagggc gaacttctct ccttcaagaa
      241 gtctccgcaa tgctccagac acggcaggtc tcgagggtct ttgaactgcg agaacttctg
      301 tgccgcggta aaggaaaatg ccgcgttgac acccgctctg aacgggaaga gaatcgaaag
      361 catagccgac cctgttgccg gggccgaaac agctcagaaa gagcacatcc aggcccatcg
      421 acgaaaggca catcgccacg ttgtacgccg aaccgccggg cctggagatc accgatgcgg
      481 tgtgtccgct accggtgatc tctatgtcct gcaggtatcc accaattacg agtattcgat
      541 tttcacttgg ttctataaag tctgtcaccg gcatctccaa gtcccggtat tatatacgca
      601 tgatcgttga gtctctcgtc tagtgccgct gtgaatactt tgacatcggg atggttcttc
      661 tggatgaatt ttacaccttc gggtgccgct ataagacaca tcagcgatat ctgcctgcca
      721 ccggctttct tcaccagctt tagtgcccag gaagaagaga cccccgtggc cagcatcgga
      781 tcgagaacga agatctgagt cgtgtcatct atcttcggca gcttcgagta gtactcgacc
      841 ggctgtatgg tgtcggggtc gcggtagatt cctatatatc ccaccgaagc gttcggcatc
      901 aacgaaagaa ccccttcgac cattcccagt ccggctctga ggattggaac gaccgtgact
      961 tttttatctt ctatcatctt cccgtgcatc tttaccagag gcgttctgat ctccttctca
     1021 taggtgggta tatgcctggt ggcctcgtag gtcaaaagaa gagttatctc tttcagcagt
     1081 tctctgaact ccttcgggcc ggtctgatcg tcgcgcatta tagtcagctt gtgctcgatc
     1141 agcggatgat agacgatggt tagttgctca aacatccagc tcactcctgc tcaccagatc
     1201 ggcgtagagc gggaacttat cggttaaatc cttcacctgg ctaccgactt ccttaaccac
     1261 tatttcgtct atttcgcctt tatctccggt aacgttttct atgaccttta cgaccagatc
     1321 ggctatcaaa ggcatttcgg cttcggtcat tcctctggtg gtaactgccg gtgttccgat
     1381 tcttataccg ctggttacga aaggcgactt cgtttctttc gggatggtgt tcttgtttac
     1441 ggttatttcg gctctttcca gagctttttc ggccgctttt cccgttacat tcttcggatt
     1501 gagatcgacg agaaagaggt gtgtgtcggt tccgccggaa acgattctga gacccctctc
     1561 ttcgaaggag ctggagagtt ttttggcgtt gttcagtatg ttttgctggt atactttgaa
     1621 ttcatccttg agtgcctcgc cgaaagaaac ggccttggcc gctatcacgt gcatcagcgg
     1681 gccgccctga gttcccggaa agatcgtttt gtctatcgat tttcctatct cttcatcgtt
     1741 ggtcaggatc atgccacctc tgggtcccct gagggtcttg tgggtggtcg tggtgactat
     1801 gtgggcaaag tccagtggat taggatagag tccggctgcg accagtcccg caaaatgagc
     1861 catatctacc atgagtaccg cgccgacttc atcggctatc tccctgaatt tcttgaaatc
     1921 tatgattctc gaatacgcgc taccgccggc cactatcact gccggtctat tttcaacggc
     1981 cagcttcctg accgaatcgt agtctataac ttcactgtcc tcgtccactc cgtaagccac
     2041 aacgttgaaa agctttcccg agaagttgac cggcgagccg tgggttagat gacctccatg
     2101 gctgagggac atgcccatta tcgtgacccc gggctcggtt accgccagat aggccgccat
     2161 attggcctgt gatccggaat ggggctgaac gttgacgaaa ttggcgttga agagacgctt
     2221 ggccctatcc ctggcaaggt tttcggcctc atccacgaaa cggcaacctc cgtagtacct
     2281 cttagcggga tacccttcgg cgtacttgtt cgtcataatc gatccgacag cctccatgac
     2341 ggctatggaa acgaaattct cggaagcgat caactccagt ccgttttcct gcctttttag
     2401 ctccttgata attatatcga atacctctct atccttttta ccaagaactt cccacataag
     2461 aaaccctcct cagtgtgtcg attcgaacat ctttgcgagt gaaacatcgt acggtggtct
     2521 tgcgattccc ttttccgtta ttatgcctgt aatcaggttc cagggtgtta cgtcgaacgc
     2581 ggggttgtaa cacttgacac cgtcgggtgc gatctgactc tccccgatat gtgtgatctc
     2641 tctgtgactt ctttcctcta taggtatctt ttccccatcg ctgcaggata gatccacggt
     2701 gctcaaaggg gcggctatgt agaaagggat tccatgattc tgggccagta tcgccagcga
     2761 gtacgtcccg attttgttgg ccgaatcacc gtttgcggca atcctgtcgg ccccgacaac
     2821 cacaagattc acgtatccct tcttcattac ccagccagcc atgttatcgc aaataagagt
     2881 cacgtcgata caagatttca taactcccag gaggtcaatc tggccccctg gaggtaagga
     2941 cgcgtctcgt ctgcataaac tctgactctc tttcccatct ctaccgccga tcttataact
     3001 cccagcgccg ttccataatc aacagttgcg agggccccgg cgttgcaatg ggttagtatt
     3061 ccgtcgccat ctctcacgac cgcggaaccg ttcttcccga tagacctgtt tgtgagtatg
     3121 tcctcccgag aaatgactat cgcctctctc tcgagtgctt ttatcaattc ctctggattc
     3181 aaagaagaca aaccggcaaa aagcctttcc atacgctcaa gcgcccagaa gagattgacc
     3241 gcggtcggtc tgctttcggc gagtctgttc ttgacggcct ccatgtgttc ggcgagctct
     3301 tccggtttct tatcgaattc catggctcca agcacgtatc caaaggctgc acttgcacct
     3361 atggccggcg cgcccctgac caccatgtcc tttatagcgc tgtaaacctc ttcgtgtgtt
     3421 cggcattcta cataaacttc ctcgaccggt agccttctct ggtctataaa tatgactctg
     3481 tcgtcgctcc attcgagagt tatgctcttg aacctggcca ctatcccttc accctctgca
     3541 agagattgta gatcatactc ctgtaggctt cctcaagccg ggag
//
LOCUS       DAOOLY010000256         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31616_length_3575_cov_2.436364, whole genome shotgun sequence.
ACCESSION   DAOOLY010000256 DAOOLY010000000
VERSION     DAOOLY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31616_length_3575_cov_2.436364"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLD15_10575"
     CDS             <1..460
                     /locus_tag="PLD15_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64397.1"
                     /translation="DIEANSARLNEYVFNGGNLIVQYHKTGEFKGSWAPYDFTISRNR
                     VTVEEAEMKILVPDHPIFNWPNVIGDSDWEGWKQERGLYFPNKWAPEFTPLIDTDDPG
                     EDIEPGSLLIANYGEGTYIYTALVWYRQLDYLVPGGYRVFSNMLSLPKVK"
     gene            675..1799
                     /locus_tag="PLD15_10580"
     CDS             675..1799
                     /locus_tag="PLD15_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPB64398.1"
                     /translation="MRETEEKIMRIVRDEGLVSRADIARKSGLSKPVVSVIVNNFISQ
                     GSVVEVKEGKSSNRGGKKPMLLSFVPTYKYVVGVDVGGNKLISILSDLDGNIVEKVRC
                     STKSISNEKTFLETVEMAISQVMKVPVSKVMGIGLGVPGTVDPESGLIFYMPAFGLRK
                     VNLRDYIEARFEVPTFVSNDVTLNALGEMWAGAAKGCRNVFLTALGTGTGAGLIINNE
                     LYEGSSGVAGEIGYMITDWSREKNLSFVFGSLESWFSGYAFEKLLSEFVNEPSVAQMF
                     DHSDTNPRFREIVTVACEHLSVVIANVITLLDPDVVVITGGIGYNQYDRIVSLIMPVL
                     KRTVPPEILERVTFKRGELGELGVSLGAVCNVQRKVFMEV"
     gene            1828..3072
                     /locus_tag="PLD15_10585"
     CDS             1828..3072
                     /locus_tag="PLD15_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64399.1"
                     /translation="MKRALLFLLVSLVVFASFAGAVKITFWQYYYETKVKLVDQLIEE
                     FEKQNPGIEVEHVTFPYENFNEKVAVSVPAGVGPDVLTLFYGWIPLYVTSGYLVPLPE
                     SEFSPEYLSSQFFPFVSKGIEFDGVPYALPTAVRSLALFWNKDLFAEAGLDPEAPPTT
                     FAEMVEFAKKLTKYDDKGNIIQEGLTMQPSGQGHHWIREVLVRQLGGTPYSTDNRTVL
                     YSLTAPDTLKVYTDLITKDKVGYPGFLNDDVTAFRSLKAAMTIDGSFRLGTFRPLENL
                     NWGVTELPSMNGVKSNFASFWAHAITTNATGEKLDASIKFLKFITSEYAQKLWLEQVG
                     ELPANPNVAAAYYDHPEYGPFLKGLEYAHATFFVDESGQRQVLMDAVDKVWIQGMDPV
                     KAFEEAAVEDQAIIDEFWSRVE"
     assembly_gap    3164..3173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3174..>3575
                     /locus_tag="PLD15_10590"
     CDS             <3174..>3575
                     /locus_tag="PLD15_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64400.1"
                     /translation="FLAIPLVFYIVVRFYPMIYTFWLSFTNWKLISPNKDFVGFDNFV
                     KLFKDKVFVTSLWNTLKYVTYGVPLVIVLSLFLAATLNRVRHLQWFYRLLYVMPYITP
                     LVAVSWVWRWIYQRPPAGILNNLLMAFGLDA"
BASE COUNT          981 a          743 c          888 g          953 t
ORIGIN      
        1 ggatattgag gcgaactctg caaggcttaa tgagtacgta ttcaatggtg gtaacctgat
       61 cgttcagtat cataagacag gcgaattcaa aggtagctgg gcgccctacg actttactat
      121 cagtagaaac agagtgacag tcgaggaagc ggagatgaag atacttgtcc cagatcaccc
      181 gatcttcaac tggccaaatg tgatcggtga ttcagactgg gagggctgga aacaggagag
      241 aggcctctac ttcccgaaca agtgggcacc tgagttcact ccgttgattg acaccgatga
      301 tcccggtgag gatatcgaac caggatcact attgattgca aactacggag aggggacgta
      361 catctatacg gcacttgtat ggtacaggca gcttgattat cttgtaccgg gtggatatag
      421 agtcttctcg aatatgttga gtttaccaaa ggtaaagtga ggcaattaga gaatccgggg
      481 gccccaaagg tccccggttc tatgaactat caaatattga gaaaactgac gataattgat
      541 tcccttcgtc cttcacaatc tgtattaagc tcagctcgcc agtatccctg tttataagag
      601 aagagagtca tgaagtataa tgttagtaag ttagcctact taacgaagca aaggtgattc
      661 cattgagtcg atgcttgcgc gaaactgaag aaaaaataat gcgaatcgtt agagatgagg
      721 gtctggtctc cagagcagat atagccagaa agagtggttt gagcaaacct gtagtgtcag
      781 taattgtgaa caatttcatc tctcagggtt cagtagttga agtaaaagaa ggaaaaagtt
      841 caaatcgcgg cgggaagaag cccatgctgt tatctttcgt tcccacttac aagtacgtgg
      901 ttggcgtaga tgtcggaggt aacaaactga tctctatact ctcggatctt gatggaaata
      961 ttgttgagaa ggtaagatgc tctactaaat ctatttcaaa tgagaagaca tttctagaga
     1021 ctgtggaaat ggcgatatct caggtaatga aagtcccagt ctcgaaagta atgggaatcg
     1081 gactcggtgt tccaggaact gtagatcctg agagcgggct aattttttat atgccagcat
     1141 ttgggctccg aaaagtgaac ttgcgagact atatcgaagc gagattcgaa gttccaacat
     1201 tcgtttctaa tgacgtcaca ctgaacgcac ttggagagat gtgggctgga gctgcaaagg
     1261 gatgcagaaa tgtctttctg acagcacttg gtacaggaac gggtgctgga ttaatcataa
     1321 acaacgagct ttacgaagga tcaagcggag ttgctggaga gatagggtat atgataaccg
     1381 actggagcag agagaagaat ctgagctttg tatttggaag cctcgaaagc tggttctcgg
     1441 gatacgcctt tgaaaagctt ctttcggagt tcgtaaacga accgtcggtc gcacagatgt
     1501 ttgatcattc agacacaaat ccgagattca gagagatagt tacggttgct tgtgagcacc
     1561 ttagcgtggt tatcgcgaat gtcatcacac tgcttgatcc agatgttgtt gtcataaccg
     1621 gtggaattgg ttacaaccaa tacgatagga tagtcagttt aataatgccg gtactaaaga
     1681 ggaccgttcc tcctgaaata cttgagcgcg ttactttcaa gcgtggagag ttgggcgaac
     1741 taggggtttc gttaggcgct gtgtgcaatg ttcaaagaaa ggtcttcatg gaagtatgag
     1801 caacatcact attttggagg tgggtaaatg aaaagagcat tgttgtttct tctggtaagt
     1861 ttggttgtct tcgccagttt tgctggcgca gtgaagatta ccttctggca gtactactac
     1921 gaaacaaaag tgaaactcgt agatcagctt atcgaagagt tcgaaaagca gaatcctggg
     1981 atcgaagtag agcacgttac tttcccctac gaaaatttca acgagaaggt cgcggtgtct
     2041 gttcctgccg gagtcgggcc tgatgttctt actcttttct acggatggat tccactttac
     2101 gttacttcgg gatatctagt tcctcttcct gaaagcgaat tttcccctga atatcttagc
     2161 agtcagtttt ttccattcgt atccaaagga attgagtttg acggagttcc ctatgccctt
     2221 ccaacggccg taagatctct agcgcttttc tggaacaagg atctatttgc agaagctggt
     2281 ctcgatcctg aggctccacc aacgactttc gccgagatgg ttgaatttgc aaaaaagctc
     2341 acgaagtacg atgacaaggg taacataatt caagaaggtc tcacaatgca gccatctggt
     2401 cagggtcacc actggattag agaagtcctt gtcagacagc tcggtggaac accctacagc
     2461 actgataaca gaacggttct ctactccctg accgcacccg acactctgaa ggtatatacc
     2521 gacctgataa caaaggacaa agtcgggtat ccaggctttc tgaatgatga tgtcacagct
     2581 ttcagatctc tcaaagcagc aatgacgatc gacggctctt tcaggcttgg aactttcaga
     2641 cctcttgaga acctcaactg gggagttacg gagcttccat cgatgaacgg agtgaagtct
     2701 aacttcgcat ctttctgggc gcacgccata actaccaatg caaccggcga aaagctggat
     2761 gcttcgatca agttcctgaa attcataacc tcagaatatg ctcagaagct ctggcttgaa
     2821 caggtcggcg agcttcccgc caacccgaat gtcgcagccg cttattatga tcatcccgaa
     2881 tacggaccgt tcctcaaggg tctcgagtac gcccatgcga ctttcttcgt tgacgaaagc
     2941 ggacaacgcc aggtcctgat ggatgctgta gacaaggtat ggatacaggg tatggatcct
     3001 gttaaggctt tcgaagaagc tgcagttgaa gatcaagcaa taatagacga gttctggtcc
     3061 agggtagagt aatttgaaga gcaggtggcc gtggcatagc cacggccgtt ctctttgggg
     3121 gtaaagaatg aagataagtc agaagagaat ccttacagct tatnnnnnnn nnntttcctg
     3181 gctatcccat tggttttcta tattgttgtg cgtttctatc cgatgatata taccttctgg
     3241 ttgagtttca caaactggaa gctaatttca ccaaataaag atttcgttgg cttcgacaat
     3301 ttcgtgaagc ttttcaaaga taaagtgttc gtaacttctc tctggaatac ccttaaatac
     3361 gtgacttacg gtgtaccgct cgtcatagtc ctgtccctct ttttggccgc tacgctgaac
     3421 agggttcgac atctgcagtg gttttaccgt ttgttgtacg tgatgcccta tatcacacca
     3481 cttgtggcag taagttgggt ttggagatgg atctatcagc ggccaccggc aggaatacta
     3541 aacaatcttc ttatggcctt cggccttgac gcaaa
//
LOCUS       DAOOLY010000257         3570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31662_length_3570_cov_3.739118, whole genome shotgun sequence.
ACCESSION   DAOOLY010000257 DAOOLY010000000
VERSION     DAOOLY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31662_length_3570_cov_3.739118"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLD15_10595"
     CDS             complement(<1..627)
                     /locus_tag="PLD15_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64401.1"
                     /translation="MKDQSLTRAKSPLARFVISNAVPILFIVLLIFAIPLSGFSTDYL
                     INEIITRLGRNSFLVISLLIPVMAGMGLNFGIVLGAMAGQIGLIFMLDWGVVGIPGIL
                     LAMLIGTPIAILLGLLAGTILNRAKGREMVTSMILGFFINGIYQLTILYGMGSLIPIT
                     SNKILLPRGYGVRNALDLISVRKVLDNMWSINISNISIPLGVFSIIIGL"
     gene            complement(624..1667)
                     /locus_tag="PLD15_10600"
     CDS             complement(624..1667)
                     /locus_tag="PLD15_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64402.1"
                     /translation="MFEKIKKLIEKAGWPRIIIGLFLLSLFFIAPFVGIKVSTSISDT
                     IVRFAMNAVLVLSLVPMVQSGCGLNFGMQLGVIAGLIGAITSIELGITGLAGFLVAIV
                     IAIPFAAILGFLYGLLLNRVKGDEMVVATYVGFSSVAFMSMMWLLLPYKSPNMIWGYG
                     GEGLRTTISVEGYWLGVLNDFLSIKVGNFFFPTGTIIFFGLMALIIWIFFKTKLGTAM
                     TAAGSNPVYARAAGVNVDRMRVLSVVISTIVAAIGILVYEQSFGFIQLYMAPLLMAFP
                     AVAALLLGGASVSKASMGNVIVGTFLFQGILTMTPSVMNNIIKSDMSEVIRIIVSNGM
                     IVYALTRRMKVRT"
     gene            complement(1660..3264)
                     /locus_tag="PLD15_10605"
     CDS             complement(1660..3264)
                     /locus_tag="PLD15_10605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPB64403.1"
                     /translation="MDEKNYLLRMEGINKDFFGNQVLKDVTLRVAKGEIVGLVGENGA
                     GKSTLMNILFGLPVIHQTGGYQGKIYLEDKEVNFASPFEAIEAGIGMVHQEFILIPGF
                     TATENILLNRESTKYNIFVEVFGERLKTLNRQEMHKRAVSAIEKLEVQLNPDMVVSEM
                     PVGHRQFTEIAREMDRKSTKLLVLDEPTAVLTESEAETMLKAARKLANLGLSIIFISH
                     RLSEVLAISDRLIILRDGQVVRELESSRTTPREVASLMVGREIKEGLSKKSKEFEKSE
                     IVLEIKDLWVDMPGEQVNGVDLQVRKGEILGIGGLAGQGKLGIANGIMGLFPAEGEVV
                     YRDEKLPLNNPVRVLGKGIAFVSEDRRGVGLLLDEPIDLNIVFTAMQIQGRFIKKLMG
                     GLIKWRDDKEIARVAREYVDSLQIKCVSEKQNAKELSGGNQQKICLAKAFVLEPDLLF
                     VSEPTRGIDVGAKSVVLETLKRQNREHGTTIVMTSSELEELRSICDRIAIVSEGKIVG
                     ILPPTADPADFGLLMLGEQEEVKASV"
     gene            complement(3341..>3570)
                     /locus_tag="PLD15_10610"
     CDS             complement(3341..>3570)
                     /locus_tag="PLD15_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64404.1"
                     /translation="AKNVIEGKCAIDDFDAVMAVFAEYTPGAGWNGSYYIDADGIERE
                     NFLMIYQDTYIFGKGYMGITEVEVPQKYLEF"
BASE COUNT          916 a          951 c          734 g          969 t
ORIGIN      
        1 caaaccgatt attatcgaga atacaccgag tggtatggaa atgttgctga tattgatcga
       61 ccacatattg tcgagtacct ttctcacgct gatcagatcg agggcgtttc tcactccgta
      121 acccctcgga agtagtattt tgttgctggt aatcggtatt aagcttccca taccatacag
      181 aatcgtcaac tgataaatac cgttgatgaa aaaaccgaga atcatagacg ttaccatttc
      241 tctaccttta gccctgttca ggatcgtacc cgccagcagt cccagaagaa ttgcaatcgg
      301 cgtgcctata agcatcgcca gcaagatacc tggtatgccc acaacgcccc agtctagcat
      361 gaagatgagc cctatctgac cggccattgc tccgagtacg ataccgaagt tcagccccat
      421 tccggccatt acaggtatta aaagagagat gaccagaaag gagtttctac ccaatcttgt
      481 tattatttcg ttgataagat aatcggtaga aaagcccgaa agtggtatag cgaatattag
      541 cagtactata aaaagaatag gaacggcgtt ggaaatcaca aatctagcca gcggactttt
      601 cgcacgggtc aggctttgat ctttcatgtt ctcaccttca ttcttcttgt cagagcgtac
      661 acaatcatac cgttggaaac gattatcctt attacctcag acatatcgct ctttatgatg
      721 ttgttcatga cagaaggtgt catcgtaaga ataccctgaa acaggaacgt tccgactata
      781 acgtttccca tcgacgcttt ggagacagag gcaccaccta gcaggagcgc agcgaccgcg
      841 gggaaggcca ttagtagagg cgccatatag agttgtataa agccgaaact ttgttcgtaa
      901 acgaggattc cgatcgcggc tacgatagtc gagataacga cagaaagcac tctcatcctg
      961 tcaacattta cgccggccgc cctggcgtaa accgggttcg atcctgccgc cgtcatggca
     1021 gtaccgagtt tcgttttgaa gaaaatccag atgatcaaag ccataagccc aaaaaagatg
     1081 atcgtaccgg tcgggaaaaa gaaatttcca acctttatac ttaggaagtc gttcaaaacg
     1141 ccaagccagt aaccttcgac acttatggta gttctcaggc cctcgccacc gtaaccccag
     1201 atcatgttcg ggcttttgta gggcaggagc agccacatca tactcataaa agcgaccgaa
     1261 gagaatccga cgtaagtcgc aacgaccatt tcatcgccct taaccctgtt tagcagtaat
     1321 ccatatagga accctagaat tgccgcaaag gggatcgcga taacgattgc gaccaggaaa
     1381 cctgccaatc cggtaatccc gagctctatg ctggttatgg ctccaatcag tccggcgata
     1441 actcctagtt gcattccaaa gttcagtccg cacccagatt gcaccattgg aacaagcgaa
     1501 agcaccagga ccgcgttcat tgcgaacctg acgatcgtgt cgcttataga agtgctcacc
     1561 tttattccta cgaacggtgc tataaaaaac agagacaaca ggaacaatcc gatgattatt
     1621 ctaggccagc cagccttttc gatgagcttt ttgattttct caaacactgg ccttcacctc
     1681 ttcctgttct ccgagcatca atagtccaaa atcggccgga tcggcagttg gaggtagaat
     1741 accgactatc ttcccttcac tgacgatggc tatcctgtcg cagatcgatc gcagttcttc
     1801 aagctccgaa gaagtcatta cgattgtcgt tccgtgttcc ctgttctgtc ttttgagagt
     1861 ctccaggacg acgcttttgg caccaacatc tatacctctg gtaggttcgg aaacaaatag
     1921 aagatcgggt tccagaacga aggctttggc aaggcagatt ttctgctggt ttcctcccga
     1981 aagctctttg gcattttgtt tctcactcac acacttaatc tgcaaagaat caacgtactc
     2041 tctggctacc ctggcgatct ccttatcgtc gcgccatttt atcaacccgc ccatcagttt
     2101 cttgatgaac cttccctgta tctgcatggc agtgaagact atgttgagat caatgggttc
     2161 atccagcaag aggccgactc ccctcctgtc ctcggatacg aatgctatac ccttgcccaa
     2221 tactctcact gggttgttca agggcagttt ttcgtctctg taaactacct ctccctcggc
     2281 tgggaagaga cccattatcc cattggctat tcccagtttg ccctgaccgg cgagtccacc
     2341 tattcccaga atttcgccct tacgtacctg tagatcgacc ccgtttacct gttcgcccgg
     2401 catatcgacc cacagatcct ttatctccag aactatttct gacttttcga actctttcga
     2461 cttttttgac aggccttcct ttatctcgcg accaaccatc aacgaagcga cctccctggg
     2521 ggtggtcctg gaagattcga gttcacgaac cacctgtcca tccctgagaa tgatcaatct
     2581 gtcggatata gcaagaacct cgctcagcct gtgacttatg aagatgatag acaaaccgag
     2641 gtttgcgagc tttctggcag ctttcaacat cgtttctgcc tccgattcag ttagaacggc
     2701 agttggttcg tctaaaacca gcaatttcgt agattttctg tccatttctc tagcaatttc
     2761 agtgaactgt ctgtgtccga ccggcatttc gctgaccacc atgtcgggat tgagctgaac
     2821 ctctaatttc tcgattgctg aaacggctct cttatgcatt tcttgcctat tcagagtttt
     2881 taatctctct ccgaagactt ccacgaaaat gttgtattta gttgattctc tattgagaag
     2941 gatattttcc gttgccgtga accccggtat cagaatgaac tcctggtgga ccattccaat
     3001 tccggcttct atggcttcga aaggactcgc gaaattcact tctttatctt cgagatatat
     3061 ctttccctgg taaccacccg tctggtgaat aacgggcaat ccgaacagta tgttcatcaa
     3121 cgtggacttc ccggcgccgt tttcgccaac cagacccact atctctccct tagcgactct
     3181 caaggttacg tctttcaaaa cctgattacc aaaaaaatcc ttattgatcc cttccattct
     3241 caacagatag tttttttcat ccataacttc accccaaact ccgtaagttg agaagagtca
     3301 agaaaagggg ggtcgccccc ccttcttgac gctatttcaa ttaaaattca aggtatttct
     3361 gtggaacctc gacttccgtg attcccatgt aacccttacc gaagatatag gtatcctggt
     3421 aaatcatgag gaagttctct ctctcgattc catcggcatc gatgtagtag cttccattcc
     3481 agccggctcc cggtgtgtac tcggcaaaaa ccgccattac cgcgtcgaag tcgtcaatag
     3541 cacacttgcc ttcgattaca ttctttgcgt
//
LOCUS       DAOOLY010000258         3553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_31855_length_3553_cov_4.458548, whole genome shotgun sequence.
ACCESSION   DAOOLY010000258 DAOOLY010000000
VERSION     DAOOLY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31855_length_3553_cov_4.458548"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLD15_10615"
     CDS             <1..223
                     /locus_tag="PLD15_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64405.1"
                     /translation="GSKPKDDDWLLPLPSFYTLSLMTIHENDALSESVIMGSKGPGNW
                     CQRYFYDRQVLYNSNSQLSGKIEYKGVFS"
     gene            220..1014
                     /locus_tag="PLD15_10620"
     CDS             220..1014
                     /locus_tag="PLD15_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HPB64406.1"
                     /translation="MKVFISADIEGTAGIASWDETNKGHADYTYFSRQMTAEVKAAIS
                     GAMKAGASEILIKDAHGSARNIDPSVLPDSVRIIRGWARDPFMMMQGIDGSFDAVVFT
                     GYHSPSGRGLNPLSHTMTGDIFSLKINGELVSEFLMNAMSAAYYKVPVVFLSGDSGIA
                     KIARDKVPDIETVVTNTGSGSSVDSIHPAKVVKMIEEGVSRALSTGSIKPIVLPEKFE
                     VEVVYVEHTQAHRLSFFPGVEKVDDRTLTFTESDYFEVLRKLLFLL"
     gene            1106..1438
                     /locus_tag="PLD15_10625"
     CDS             1106..1438
                     /locus_tag="PLD15_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPB64407.1"
                     /translation="MSNYHEPYEALNEKARNISRALNSLKEEIEAIDWYNQRVEASSD
                     PELKAIMAHNRDEEIEHACMALEWLRRNMNGWDEELRTYLFTSVPVTEAEETGESAGN
                     GRLKIGKL"
     gene            1452..2246
                     /locus_tag="PLD15_10630"
     CDS             1452..2246
                     /locus_tag="PLD15_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HPB64408.1"
                     /translation="MDIFKRQLAPLSGEAWKEVNDRAGQVLKNYLSARKVVNVVGPKG
                     WEYSFLPEGRLDLIETGSEVGAGLRKSKPLVELRVPFRLNRWELDNIDRGTADPDLGP
                     LEEAARKIALFEENTIYNGFEAGMIKGLVREARTEPVSLGKKPEEIVKSLARAVHKLK
                     DNFADGPFALVVNPETLAMLNSHVQGYPLVKRIESLLGTDIVVSRVLQGGLLLPKDHD
                     DLEMVIGGDFEIGYHGHTDEEVELFIAESFTFRVLDPNIIVNIIVN"
     gene            complement(2305..>3553)
                     /locus_tag="PLD15_10635"
     CDS             complement(2305..>3553)
                     /locus_tag="PLD15_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HPB64409.1"
                     /translation="TKFDEEGVKDTYSKFFGTLQSVYAFFTMYANIDGVDPVDFELPV
                     KGREEIDRWVISRLNTLIGQVTGFLNGYDLTKAVRAIQDFVVEEVSNWYVRRTRDRYW
                     TSELDSSKKGAYLTLYEILLTVARLMAPIAPFTAEEIYRNLTHGKEGSIHLQFYPETD
                     KSLIELDLEKRMATVMDLVTLGRACRNKVQIKVRQPLPKILVDGGIKPTVESMRELIL
                     EEINVKKIEYIEDLGKYVKYEVKPNFRVMGPKFGKDLKAIGAALKAQEAAEIVSSLKR
                     EGKLLLEIGGNSFELMEDDLDIRIQELAGFTFEMSADNFVILDTELTPDLLQEGLARE
                     MVSKIQTMRKDADFKITDRIRVFYSGPEDVVRAIMVFESYVKEETLADSLTPGDGVQG
                     GTEWNLNGYDTVIKVERIAKGSI"
BASE COUNT          888 a          881 c          910 g          874 t
ORIGIN      
        1 aggctctaag cctaaggatg acgactggct actccctcta ccctcattct atactctctc
       61 tcttatgaca atacacgaga atgatgcatt aagtgagtcc gtcattatgg gaagtaaagg
      121 accgggcaat tggtgtcagc gctattttta cgacagacag gtattatata attcaaatag
      181 ccaactatcc gggaaaatag aatacaaggg ggtcttttca tgaaggtatt tattagtgca
      241 gacatcgaag gaacggccgg gatagcttcc tgggatgaaa caaataaggg tcacgccgat
      301 tacacgtact tctcaaggca gatgacggcc gaagtgaagg ccgcaataag cggggctatg
      361 aaggccggcg cctcggagat attaataaaa gacgctcacg gtagcgccag gaacatcgac
      421 ccgtcggtac ttcccgatag cgtaaggata atcaggggtt gggcccgaga tcctttcatg
      481 atgatgcagg gaatagacgg ctccttcgac gccgtcgttt tcaccggcta ccattcgccg
      541 tccgggagag gactaaaccc tctttcgcac acgatgacgg gagatatctt ctctttgaaa
      601 atcaatgggg aactggtgag cgaatttctc atgaacgcta tgtcggcagc atattataag
      661 gttccagtcg tctttctgag cggagattcg gggattgcaa agatcgcccg tgataaagtt
      721 ccagatatag agacggtcgt aactaacacc ggaagcggtt cgtcggtcga ttcaatccat
      781 cctgcgaagg tcgtcaaaat gatcgaagag ggcgtctcta gagcgctttc aaccggctcc
      841 ataaagccga ttgttcttcc ggaaaagttc gaagtagagg tcgtgtatgt agaacacaca
      901 caggcccaca gattatcctt cttcccaggg gtcgagaaag tagatgacag aacgctaacc
      961 tttaccgaga gtgactactt cgaggtgttg aggaagctac tgttcttact gtagctgaaa
     1021 cggtgctttt atagcttaaa cccgaatgtg ttctgtccag tcatacaatg gtaaggctca
     1081 ccgaatttag aaaaggaggt tctttatgag caactatcat gaaccgtacg aagcgcttaa
     1141 tgaaaaggcg aggaatatct ccagggcttt gaacagcctc aaggaggaaa tcgaagccat
     1201 agactggtac aaccagagag tcgaggcttc gtctgaccct gaactgaagg ctatcatggc
     1261 ccacaaccgt gacgaggaga tcgagcacgc ctgcatggcc ctcgaatggc tcaggagaaa
     1321 catgaacggc tgggacgaag agctcaggac ttatctcttc acttccgtac cggttacgga
     1381 ggcagaagag accggcgagt cggcgggtaa tggccggttg aaaataggaa agctataagg
     1441 agggaatgag cgtggatata ttcaaaagac agctcgctcc gctttcgggc gaggcatgga
     1501 aagaagtcaa cgacagggcc ggccaggttc tgaagaacta tctttcagcc agaaaggtgg
     1561 ttaacgtcgt gggaccgaag ggctgggaat attccttcct gcccgaggga aggctcgatt
     1621 tgatcgaaac ggggagcgaa gttggcgccg gtctcaggaa gtcaaaacct ctggtggaat
     1681 tacgagtccc tttcagattg aaccgctggg aactggataa catagacagg ggaacggccg
     1741 atcctgatct cggaccgctt gaagaggccg ccagaaagat agcacttttc gaagagaaca
     1801 caatatacaa tggtttcgaa gccggcatga taaagggtct cgttagggag gccagaaccg
     1861 agcctgtatc gctgggcaaa aaacccgaag agatagtgaa gtcgcttgcc agggccgttc
     1921 ataaactgaa ggacaacttc gccgacgggc ccttcgctct ggttgtgaac cccgaaacac
     1981 ttgcgatgct caactcccac gtacagggtt accctctcgt caaacgtatc gaatccttgc
     2041 tgggaacgga catagtcgtg agcagggtcc tccagggtgg tctgcttctt cccaaagatc
     2101 atgatgacct ggaaatggta atcggcggag acttcgaaat tggctatcac ggccacaccg
     2161 atgaagaagt ggaactattc atagctgaat ctttcacttt cagagtgctc gatccgaaca
     2221 tcatcgtgaa tataatcgtc aactgataaa aaaaccgggg gaaaagctcc cccggttttt
     2281 tattgcctaa agctaagaga atcactatat cgatcccttc gcgattctct cgacctttat
     2341 cactgtgtcg taaccgttca gattccattc tgttccgccc tggactccgt cacctggagt
     2401 gagactatca gcgagagttt cttccttcac gtaagactcg aagaccatta tagccctgac
     2461 cacatcttcg ggtccagagt agaaaaccct tatcctgtct gttattttga agtcggcgtc
     2521 ctttcgcatc gtctgtatct tggagaccat ctccctggcc agtccctcct gcaataaatc
     2581 cggcgtcaat tccgtgtcga gtatgacgaa attgtcggcg ctcatttcga aggtgaatcc
     2641 tgccagttcc tgtatcctta tatccagatc atcttccatc agctcgaaac tgtttccgcc
     2701 gatttcgagg aggagttttc cctctcgctt gagcgatgag acgatttcgg ccgcttcctg
     2761 ggccttcagc gccgcaccta tcgccttgag gtctttgccg aatttcggac ccattaccct
     2821 gaagttgggt ttcacttcgt atttcacgta cttgcccagg tcttctatgt actcaatctt
     2881 cttaacgttg atctcttcta gtatgagttc cctcattgac tccacggtgg gtttgatgcc
     2941 accgtcgaca aggatcttgg ggagcggctg tctaaccttt atctgcacct tgttccggca
     3001 ggctcttccg agggtaacca ggtccatgac cgtcgccatt ctcttctcca ggtccagctc
     3061 gattagagat ttatccgttt caggatagaa ctgtagatgg atcgatcctt cctttccgtg
     3121 agtaaggttt ctgtatatct cctcggcagt gaagggcgct atcggtgcca taagcctggc
     3181 cacggtgaga agaatctcgt acagcgtcag gtaggccccc ttcttgctcg agtcgagttc
     3241 gctcgtccag tacctgtctc tggttcttcg aacgtaccag ttcgagacct cctcgacgac
     3301 gaagtcctgt atcgccctga cggctttggt gagatcgtaa ccgttcaaaa agcctgttac
     3361 ctgacctata agtgtgttga gccgggaaat gacccagcgg tctatctctt ccctcccctt
     3421 cacgggtagc tcgaaatcga ccgggtcgac accgtctatg ttcgcgtaca tggtgaagaa
     3481 ggcgtaaacg ctctgtaggg tgccgaagaa cttgctgtag gtatccttga caccctcttc
     3541 atcgaatttg gtg
//
LOCUS       DAOOLY010000259         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_32122_length_3531_cov_4.402186, whole genome shotgun sequence.
ACCESSION   DAOOLY010000259 DAOOLY010000000
VERSION     DAOOLY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32122_length_3531_cov_4.402186"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..375)
                     /gene="rpoZ"
                     /locus_tag="PLD15_10640"
     CDS             complement(142..375)
                     /gene="rpoZ"
                     /locus_tag="PLD15_10640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPB64410.1"
                     /translation="MIINYDSLLKRIRHKYAIPVAAAKRAEDLEDFGRPKLDSATVKA
                     AGDKITVALKELEEGYIRIRNEEMLMILVPKVK"
     gene            complement(372..989)
                     /gene="gmk"
                     /locus_tag="PLD15_10645"
     CDS             complement(372..989)
                     /gene="gmk"
                     /locus_tag="PLD15_10645"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPB64411.1"
                     /translation="MKGLVFVMSGPSGAGKTTILKEILARNDNLVFSVSYTTRKIRPG
                     EIDGKDYFFVSEETFKSLIEGKELLEWAKVHGNCYGTSKEFVSSCTDSGRDVLLDVDI
                     QGAISIMKNLEDAVYIFIAPPSYNELVRRLTSRGTESSESLKVRLEDAKWELEQVKRF
                     EYLVVNNNLKQSVSQFEAVITAERLRVDRIVRERGEKFLFEEPVE"
     gene            complement(986..1294)
                     /locus_tag="PLD15_10650"
     CDS             complement(986..1294)
                     /locus_tag="PLD15_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPB64412.1"
                     /translation="MFFLYGLINVGFGNVIIGDRVIAIVNPESAPLKRLKEVAKDEGK
                     LIDATYGRKTRAIVITDSNHIILSAIQPETIASRFMQTFSDIEKLLEEIRESGQSFEE
                     "
     gene            complement(1301..2173)
                     /locus_tag="PLD15_10655"
     CDS             complement(1301..2173)
                     /locus_tag="PLD15_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPB64413.1"
                     /translation="MIRSMTGYSRAESVVDGINVLVELKTVNSKYLNLDVNSGETFAE
                     LELDVSRYIKERLKRGTVKARVEISLIEDSGLLKPDFGTATSIYASLKAIADRLGLGG
                     EVSIDSMTRFKEILRSRPSEELARRVWNAVVPVLEKALESLNRDREREGLNLLKALED
                     YLDKLQQISSQLKEMSDGMVDYYREFLKKRVEKLFDGELDENRLEQEVVLLAEKADIS
                     EELVRLDSHIASFRETLSNDGDCGVQLDFICQELHRELSTVASKSKKLEISALSVEGR
                     TLVNKLREQVQNIE"
     gene            complement(2230..3357)
                     /locus_tag="PLD15_10660"
     CDS             complement(2230..3357)
                     /locus_tag="PLD15_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPB64414.1"
                     /translation="MICYFDNNATTAMDRDLAGLMLSLCTEEYGNPNSIHSMGIRANK
                     MLEEARLRVAKVLLADPREIFFTSSATESINWILRSCVAFRGKRKKVVTSAIEHKAVL
                     NTLKDLKNTHEIEVVEVKPGRDGVIDSRKMLEMVDEETFLVSLMAVNNVTGSIQPFEE
                     VGKVLRDRGVLYHVDAVQTIGKYPFAPAEAFCDFASFSAHKFHGPKGVGITYVKRGAS
                     IRAVVTGGGQEKALRSGTQNVPGIVVSSMAMERAVNSMEKTTTKLKGLKERLRLEVEK
                     IGGVVNTAERSVSNTLNVSFPGVKSEVLANALSTDGVYVGISSACSSRSDSGQYVLDS
                     MGLGAGIASSSLRISMSKFTTVEELEILVERLKKSVSLLKF"
     gene            complement(3354..3521)
                     /locus_tag="PLD15_10665"
     CDS             complement(3354..3521)
                     /locus_tag="PLD15_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64415.1"
                     /translation="MHELISSLGGEVAASVSRNTDYLLAGENAGSKEAKARSLGIKVI
                     TESEFEEMIDL"
BASE COUNT          814 a          983 c          739 g          995 t
ORIGIN      
        1 tgattacaaa tcagccgctc ttccgattga gctacgttgg cccgacgcac gatatttatt
       61 ataaccaatg actggagccc tgtcaacaag gaggagaaaa ataagatgac tacccttgct
      121 ggacgcagat ctcccggaaa gttactttac ttttggcacg aggatcatca acatctcctc
      181 gtttctgatt cttatataac cctcttccag ctccttaagc gcgacggtga tcttgtctcc
      241 ggccgctttt accgttgccg aatcgagttt aggtctgccg aagtcttcga gatcttcagc
      301 cctctttgct gctgcaactg gaattgcgta cttgtgcctt attctcttca gtagagaatc
      361 gtagttaatt atcattcaac tggctcctca aaaagaaatt tctcgcctct ttccctgacg
      421 attctatcaa cccgcagtct ttcggctgtt attaccgctt caaattggct taccgactgt
      481 tttaagttat tgttaactac cagatattcg aagcgcttga cctgttccag ctcccatttg
      541 gcgtcttcga gacgaacttt aagcgattcc gaactctccg tccctctgga ggtgagtctt
      601 ctgaccagct cgttgtaaga aggaggcgcg atgaagatgt atactgcgtc ttccaggttc
      661 ttcatgatgg aaatggcacc ctgtatatcc acgtccagaa gaacatcacg accgctatcc
      721 gtacagctac tgacaaactc tttcgaagtt ccgtagcagt taccgtggac cttcgcccac
      781 tcgagcagtt ctttcccctc gatcaaagat ttgaaggtct cctcggagac gaagaaataa
      841 tcctttccat ctatctctcc gggtcttatc tttctagtgg tgtaggaaac cgaaaaaacc
      901 aggttgtcgt ttctagcgag aatttccttc agtatggtcg tctttccggc ccccgacggg
      961 ccgctcatca cgaagactag tcccttcatt cctcaaaact ctgtcccgat tcccggattt
     1021 cctcgagaag cttctcgatg tcgctgaagg tctgcatgaa tctggaagct attgtctcgg
     1081 gttgaatcgc cgagagaata atgtggttgc tgtcggttat gacgatcgcc ctggtttttc
     1141 taccgtatgt agcatcgata agtttcccct cgtcttttgc gacttccttc aatctcttca
     1201 gcggagcaga ttcagggttg actatcgcga tcaccctgtc tccaataatg acatttccga
     1261 aacccacgtt aatgagtccg tacaagaaga acacctccca ctactctatg ttctgcactt
     1321 gctccctcag cttgttcacc agagtcctgc cctcaacgga cagagcagat atttcgagct
     1381 tctttgattt cgaagccacc gtcgataact ctctatgtaa ttcctggcag atgaaatcca
     1441 gctgcacgcc gcagtcgcca tcgttggaca gagtttctct gaaagacgcg atatgagaat
     1501 ccagtctcac cagctcttcg gatatatcgg ccttttccgc cagaagaacc acttcctgtt
     1561 ctagtctgtt ctcatcgagc tctccgtcga aaagcttttc gactctcttc ttgagaaact
     1621 ctctgtagta atccaccata ccgtccgaca tctctttcaa ctgcgatgat atttgctgta
     1681 acttatcgag ataatcttcc agggccttca agaggtttag tccttcccgt tcgcgatcgc
     1741 gattgagtga ttccagagct ttctccagaa ctggaaccac ggcattccat actctcctcg
     1801 ccagttcttc ggaaggtctg ctcctgagta tctccttgaa cctcgtcatt gaatctatcg
     1861 agacctctcc gccgagcccg agtctgtcgg ctatagcctt cagagaggcg taaatcgatg
     1921 tcgccgttcc aaaatccggt ttcaaaagac cgctatcttc tatgagcgaa atctccactc
     1981 tggctttcac agtacctctt tttagtcttt cctttatgta ccttgaaaca tctagttcca
     2041 gttcggcgaa ggtctctccc gagttgacgt cgaggttcag gtacttcgaa ttaactgtct
     2101 tgagttcaac aagaacgtta ataccgtcaa ccacactctc ggctctcgaa taaccggtca
     2161 tacttctgat caaaagaatc acctcaaaga cagttacgtc taactacagt caattataac
     2221 cgttaagagc taaaatttca aaagagagac cgatttcttc agtctttcta ctagaatctc
     2281 aagttcttca accgttgtga acttcgacat acttattctc aaagaactgg aagctatccc
     2341 ggcacccagt cccatgctat ccagaacgta ctgaccgctg tcgcttctgg aagagcaggc
     2401 cgaagatatc cccacataaa cgccgtcggt ggagagagca ttggcaagga cttcggattt
     2461 cacacctgga aaagagacgt tcaaagtgtt gctgaccgat ctctcagcag tgttgacaac
     2521 ccctccgatc ttctcgacct ccagcctcaa cctctcttta agtcccttga gtttcgtcgt
     2581 tgtcttctcc atcgaattga cggccctttc catggccata gaggaaacca caattcccgg
     2641 aacgttttga gttccagacc gcagggcctt ctcctgtccg cccccggtta caaccgcacg
     2701 tatggatgcg ccgcgtttca cataggtaat gcccacgcct ttcggtccat ggaacttgtg
     2761 agccgaaaag gaggcgaagt cgcagaaagc ttccgccggt gcgaagggat atttccctat
     2821 agtctggacg gcatcgacgt ggtatagaac ccctctgtct ctcagcactt ttcccacctc
     2881 ttcgaaaggc tggatggaac ctgtgacgtt gttcaccgcc atgagagaga ccagaaatgt
     2941 ctcttcatcc accatctcga gcatttttcg actatcgatc accccgtcgc gaccaggctt
     3001 aacctccaca acttcgatct cgtgggtgtt tttcagatcc ttaagggtgt tcaaaactgc
     3061 tttgtgttct atcgccgatg tgacgacctt tttcctcttg cccctgaagg caacgcacga
     3121 gcgtaatatc cagttgatcg actcggtcgc gcttgaggtg aaaaagatct ctctgggatc
     3181 tgccaggaga actttggcta ctctcaaacg tgcctcttcc agcatcttgt tcgcccttat
     3241 gcccatagag tgaatagagt tcggatttcc gtactcttcg gtgcagagac tcagcattag
     3301 cccggccaga tctctatcca tggccgtggt tgcgttgttg tcgaaatagc agatcaaaga
     3361 tcgatcatct cctcaaactc gctctcggta atcaccttta taccgagaga tctggccttc
     3421 gcttctttgg aaccggcgtt ttctcccgcc agaaggtaat cggtgttcct ggaaaccgaa
     3481 gccgccacct ctccgccaag ggaagagatt aactcgtgta tcgcgtttct t
//
LOCUS       DAOOLY010000260         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_32719_length_3484_cov_2.930884, whole genome shotgun sequence.
ACCESSION   DAOOLY010000260 DAOOLY010000000
VERSION     DAOOLY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32719_length_3484_cov_2.930884"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2018
                     /gene="recG"
                     /locus_tag="PLD15_10670"
     CDS             <1..2018
                     /gene="recG"
                     /locus_tag="PLD15_10670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPB64416.1"
                     /translation="SVGESRSKILKRMGIETVGDLVNYFPRDYEDRRTIVPLSSIAGE
                     EKVSIKGKLLNYSVKKVSGYTIISAVVSDGFGQILLKWFNQDYILQRLVKDRVYLIHG
                     TAKKTPFGPLEMNSPEIEKIEGEVPREILPVYSLTSGISMNMMRKIMKRNLGVVKSLD
                     ELLPDFVRHERDLMSRRHAMISIHFPLSLFEMTRARKYLVYEEFFLFELAILYNRSQL
                     RKSRGGIAKTITGELSKRLIARLPFELTGDQIKVFEEIREDMRSSNPMNRLLQGDVGS
                     GKTMVAELAMVDNFEAGYQSALMVPTSVLAMQHFEKLSSDLEPLGIKVELLTGSLKKS
                     EQDRIRLGLVMGEIDIVIGTHSLIQEGVDFENLGLIVVDEQHRFGVRQREALTTKGHL
                     VDSLIMTATPIPRTLAMTAYGDLDVSTIMTMPKGRNPVRTLLISRSRTRELYSFIMEE
                     LKMGHQIFFIYPLVEESEQIDLKNATDEAERLRSEIFPDVGVELLHGRMSDLEKQEVM
                     GRFRSKESMILVSTTVVEVGIDVPSATVMVIEHPERFGLAQLHQLRGRVGRSSLKSYC
                     IMVLNSGVSKEALDRLRDFSSTSDGFTVAELDLKLRGPGEFMGLRQHGLPQFSLGDIV
                     RDSDLLTAAREDAAKLLRSDPDLQSSGALRLEIEKRFSDSINLIEVG"
     gene            2019..2594
                     /gene="rsmD"
                     /locus_tag="PLD15_10675"
     CDS             2019..2594
                     /gene="rsmD"
                     /locus_tag="PLD15_10675"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPB64417.1"
                     /translation="MLTLTGGEWSRRRIEVTEREVTRYTPQIARKAIFDIIDVEGKNF
                     LDAFSGSGVMVFEALSRRAKQATAIDVSHTACKTIRRNMDRLDPSMKLEILCGDFRRM
                     LPLLAEREERFDFVFADPPFDRNYMPSFLKSLSENISILNLGSLIIVETSEGESLFEE
                     REEARFLKLIDRRNYANVVFTFLVMSNEVPR"
     gene            2578..3057
                     /locus_tag="PLD15_10680"
     CDS             2578..3057
                     /locus_tag="PLD15_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPB64418.1"
                     /translation="MKFLVDESLSKLGRKLRILGLDTAVCSWQTFDSLIVERDIFLTT
                     SRRFLERAIKSRLRCYLLKSNSWRSQLKSVFQRYSIRVEDLKPFSRCSVCNGELEEAS
                     PEDLCSVPEYVLLNNENLRKCQNCGRVYWKGSHVEHMRSLYRIIAETSGGKTEQSPF"
     gene            3089..>3484
                     /locus_tag="PLD15_10685"
     CDS             3089..>3484
                     /locus_tag="PLD15_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB64419.1"
                     /translation="MKRISEYFSGDVSLYILEKYKGVYKEIIFGEKLQRSGREIRLES
                     NESLEEFTTGVFSGTVTVHSHSDSAEESRDLHFLNHEGDLLGLLSLPRGSYVASSLKE
                     WISELEMALHYSLTRNYEQEGYRRLKLVSR"
BASE COUNT          930 a          757 c          959 g          838 t
ORIGIN      
        1 cctctgtcgg cgaatcgagg tcgaagatac tcaagcgtat gggaatagag accgtgggag
       61 atctcgtgaa ctatttcccg cgcgactacg aagacaggag aacgatagtg cccttgtcct
      121 cgatagccgg cgaagagaag gtcagcataa aggggaaact cctgaattat tcggtgaaaa
      181 aagtctcggg ttacaccatc atttcagccg ttgtgagcga tggctttggt cagattcttc
      241 tcaagtggtt caatcaggac tatattctgc agagactggt taaggaccgt gtatatctaa
      301 tacacggaac cgcgaagaaa acacctttcg gtccgctgga aatgaactcg ccagaaatag
      361 agaaaattga gggagaagta ccccgggaga tccttccggt ttattctctg acctcgggca
      421 tatccatgaa tatgatgcgc aagataatga aaagaaacct cggggtagtt aagtcgctcg
      481 atgagttgtt gccagatttc gtcaggcatg agagagattt gatgtctaga agacatgcga
      541 tgatttcgat ccactttccc ctcagtcttt tcgagatgac gagggccaga aagtacctcg
      601 tctatgaaga gttcttccta ttcgagctgg ccattcttta caaccgttcc cagctgagaa
      661 agagcagggg aggtatcgcg aaaaccatca ccggtgaact ctcgaaaaga ctgattgcga
      721 gattgccttt cgaacttaca ggcgatcaga tcaaagtctt tgaagagata agggaagata
      781 tgagatcttc caaccctatg aataggctgc tccagggtga tgtcggttcc ggcaagacca
      841 tggtggccga actggcaatg gtggacaatt tcgaagccgg ttaccaaagt gctttgatgg
      901 tgccgacttc tgttctggcg atgcagcatt tcgaaaagct gtccagcgat ctcgaaccgc
      961 tcggaataaa agtcgaacta ctcaccggct cgctcaagaa gagcgagcag gatcgaatca
     1021 ggctcggtct agttatgggg gagatagaca tcgttatcgg cacgcactca ctgatacagg
     1081 aaggcgtcga tttcgaaaat ctgggcttga tcgttgtaga tgaacagcac agattcgggg
     1141 tgagacagag ggaggcactg accacgaagg ggcacctcgt cgacagcctc atcatgaccg
     1201 cgacgcctat tcctaggacc ttagccatga cggcgtatgg cgatctcgat gtatcgacca
     1261 tcatgaccat gcccaagggg cgaaacccag tgaggacact actaatctcc agaagcagga
     1321 cgcgcgagct gtattctttc ataatggaag aactgaaaat gggacaccag atcttcttca
     1381 tctatccgtt ggtggaggag tcggaacaga tagatcttaa aaacgctacc gacgaagcgg
     1441 agaggttgag aagcgaaatc tttccggatg tgggcgtaga gcttctgcat ggaagaatga
     1501 gcgaccttga gaaacaggaa gttatggggc gttttcgctc gaaggagagc atgatactcg
     1561 tttcgacgac agtggtcgaa gtcggcatag atgtaccctc cgcaacagtt atggtgatcg
     1621 aacacccgga gagatttggg ctggcgcagc tccaccagct cagaggaagg gtcggaagga
     1681 gttcgttgaa atcttactgt atcatggtct tgaacagtgg tgtatctaaa gaagcgctcg
     1741 acaggttgcg cgatttctcc agtacgtcag acggtttcac ggttgcggaa cttgatctta
     1801 aattgagggg tccgggcgaa tttatggggt tgaggcagca cggtttgccg caattctcgc
     1861 ttggtgacat agttagagac tccgatctac tcacggccgc tcgtgaagat gcggctaaac
     1921 tgctcaggag cgatccggat ttgcagagtt cgggtgcatt gcgccttgag atagaaaaga
     1981 ggttttccga cagtattaac ctgatagagg tgggatagtt gttgacgttg actggcggcg
     2041 aatggtcccg tagaaggata gaggtaacgg agagagaagt gacccggtac actccgcaga
     2101 tcgctagaaa agcgattttc gatataattg atgtggaagg gaaaaacttt ctggatgcct
     2161 tctccgggag cggcgtgatg gttttcgagg cccttagtcg gcgagcaaaa caggcgacgg
     2221 cgatcgatgt ttcgcataca gcatgcaaga ctatccggag aaacatggat cgtctcgatc
     2281 cttcgatgaa gctcgagatt ctctgcgggg attttcgtag aatgctgccc ctccttgcgg
     2341 aaagggaaga gcggttcgac ttcgttttcg ccgatccgcc attcgatcgc aactatatgc
     2401 cttccttctt gaaatctctt tcggagaaca tttcaattct aaacctcggc tcgctgataa
     2461 tagtcgaaac ttcagaggga gaatcgctct ttgaagaaag agaagaggcg cgctttttaa
     2521 aactgattga caggaggaat tacgcgaacg tggtattcac gttcctggtg atgtcaaatg
     2581 aagttcctcg ttgacgaatc gctttcaaaa ctcggaagaa agcttagaat actgggactg
     2641 gacacggctg tttgcagctg gcaaactttc gactctttga tagtggagag agacatcttt
     2701 ttgactacca gcaggcgttt ccttgaaagg gccattaaaa gtcgtttacg ctgttacctt
     2761 ctaaaatcga acagctggag gagtcagctg aagtccgtct ttcagaggta ctcaattcgg
     2821 gtggaagatc taaagccttt cagtcgttgc tcggtctgca acggtgaact agaagaggct
     2881 tcccctgaag atctttgctc ggttcccgag tacgtactgc tgaataacga aaatctgcga
     2941 aagtgccaaa actgcggcag ggtttactgg aagggcagcc acgtagaaca tatgaggtcg
     3001 ctctatcgga taattgctga gacgagtggt ggtaaaactg aacagagtcc attttgaaac
     3061 ttcgtatgag aagataatac agagactaat gaagaggatc tccgagtatt tctcgggtga
     3121 tgtatctctt tacattctcg aaaaatacaa aggcgtttac aaagagatca tattcggtga
     3181 gaagctccag aggtccggtc gggagataag actcgaatcg aacgagtcgc tggaagagtt
     3241 caccaccggt gttttctcag ggaccgtcac ggtgcatagc cactcggatt ccgccgagga
     3301 gtcgagagac ctacattttt tgaatcatga gggcgatcta ctcggtttgc tctcgcttcc
     3361 acgtggctct tatgtagcca gctccctgaa ggaatggatt tcagaactgg agatggcgct
     3421 tcactattct ctgacacgta actacgaaca ggaaggttac agaagactga agctggtatc
     3481 gcgc
//
LOCUS       DAOOLY010000261         3450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_33110_length_3450_cov_4.503387, whole genome shotgun sequence.
ACCESSION   DAOOLY010000261 DAOOLY010000000
VERSION     DAOOLY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33110_length_3450_cov_4.503387"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLD15_10690"
     CDS             <1..1108
                     /locus_tag="PLD15_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64420.1"
                     /translation="EKVYEALKTRNIYASEDRNAFISFSTGRAKMGDILYDLPAATLS
                     IEYSDPGEPVASAWLYSNEGSTALEVEGDSWTVEVDVENPLSYNWYFVKIDQKDGNEI
                     VSSPIWFQSSSERYLLNARSIDARPVFGLPFEIAFDLINARRNEESLTLTIMGEKGPV
                     LEREYILPAMSCVEIKETLDCTSQDLVFYVNGEHAFGLKIDFTRFVAHLDTSHENYYV
                     SELKYLTELLSDLGGDVRPVIGELTESTLNSGQLLILPLPDQDTFMKDFMAISEKQIT
                     FIKNYVKSGGLLIVVLARKAITHDSLSTYERLFEELSWGVSLEDGGLSFEGPSEGIVH
                     RESGGLVLKWNQATGKEPPDEEIVRFMKSRLKID"
     gene            1119..1868
                     /locus_tag="PLD15_10695"
     CDS             1119..1868
                     /locus_tag="PLD15_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB64421.1"
                     /translation="MSRIESFTSGIEGNRIFGIFELPESGDSFPVVVMLHGFTGEHIV
                     STFKYPRLSRRLVESGVATVRFDFRGSGDSEGEFCDMSPLTELRDALEIVALVKEKSC
                     FNGKLALIGYSMGGMVASLLAGREREFDAVLLWSPVIMNGEFFSREEYSFSSDEKYKD
                     ILGLKLGHGFQKDGLSVDAAAELANYRGPLMIIHGSEDESVPYEPVLRYARDRGIVFH
                     TVKGANHKYQRLDWIEELFEVSEGFLCDRLL"
     gene            complement(1860..2933)
                     /locus_tag="PLD15_10700"
     CDS             complement(1860..2933)
                     /locus_tag="PLD15_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64422.1"
                     /translation="MADVRVLNNEAVSVLAALFRLNNHEKMIDRDTLAADHLARWVIK
                     SRVSIPAELFEMIEVFFSWDSFFGLRTLPYICFEGIDSCEAFMEWLERLSPQSLMAKF
                     FSPQYYEDCEARDLAFRLLELDERETLKYVFSNLELGNSSRYSAIEILRDQNGAKESF
                     SSLLHFICRELFDGEFLEILRESSRTFRELFNGNFSLYGQPFLRELLDIERREESSES
                     DLYIFSSWFLGGSVITVNLPERNSVISIVGEETMRKKNMLPEAMDPLEIAGVLSVEGS
                     MNLLKTLMNRRMNIRSIAKETGLHRHGLVEKLAALSRQGLAVTGVSGDEFWFETDEQL
                     IDRALLIMREMMVGKRVADHPLQ"
     gene            complement(2926..>3450)
                     /locus_tag="PLD15_10705"
     CDS             complement(2926..>3450)
                     /locus_tag="PLD15_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB64423.1"
                     /translation="HEVEKQRSKLHALFEKYGKNYVQWLTGSSARSGVPSTGSITIAV
                     SFFYEIILLKGSIPYRREDIYVVGYRYPERITPPVDRMASGVDLFTILANDVNLRILK
                     RLSQAPMGVTEIALSTRLTNVEVTDYLSVLMRYGVIEDSVEGDRRLFNLNKAFLVKAV
                     GDSVKEIFGEAIDG"
BASE COUNT          861 a          843 c          899 g          847 t
ORIGIN      
        1 cgagaaggtt tacgaggctc tgaagacgag gaatatctat gcaagcgagg acaggaacgc
       61 cttcatatcc ttctcgacgg gtcgggccaa gatgggcgat attctctacg atttgccggc
      121 ggctactttg agtatcgaat acagtgatcc cggtgagccc gtagcatcgg cctggcttta
      181 cagcaacgag ggatcgacgg cgctggaagt ggagggcgat tcctggacag tcgaggtcga
      241 tgtagagaat ccgctctctt acaactggta tttcgtgaag atagaccaga aggacggaaa
      301 cgagatcgta tcctcgccga tctggttcca gagcagcagt gagagatatc ttttgaacgc
      361 cagatctatc gacgccagac cggtttttgg gctgcctttc gagatagcct tcgacctgat
      421 aaacgccaga aggaacgaag agagtctcac gttgactatc atgggtgaga agggaccggt
      481 tctggagaga gaatacatcc ttccggccat gagttgtgtc gagataaagg aaactctgga
      541 ctgcacttct caagatctag ttttttatgt caacggtgaa catgccttcg ggctgaagat
      601 cgatttcacc aggttcgtcg ctcatctcga cacttctcac gagaattact atgtgtccga
      661 gttgaaatac ctgacggaac ttttaagcga tctcggcggc gatgtgagac cagtgatagg
      721 cgaactgacg gaatcaacgt tgaacagtgg ccagctgttg atccttccgc ttcccgatca
      781 ggacactttc atgaaggact tcatggccat ttcggaaaag cagatcactt ttatcaagaa
      841 ttacgtaaaa agtggtggtc tgctgatcgt tgtcctcgcc agaaaggcga tcacacatga
      901 ttcgctgagt acttatgaaa gactctttga ggaactgtcc tggggagtct cgctcgaaga
      961 tggcggtctc tccttcgaag gaccttccga ggggatagta catagggaga gcggcggcct
     1021 ggttttgaaa tggaatcagg cgacaggtaa agagccgccg gacgaagaaa tcgtgcgatt
     1081 catgaaatct agattgaaga tcgattgagg ttgagtctat gtcgagaata gagagtttca
     1141 catcgggaat cgaaggcaac aggatcttcg gaatcttcga gctccccgaa agcggcgata
     1201 gttttcctgt ggtggtgatg cttcacggct tcaccggaga gcatattgtt tcgaccttca
     1261 aatatccgcg actttcaaga agactcgtgg aaagcggtgt cgcgaccgta agattcgact
     1321 tccgtggttc gggtgacagc gagggcgagt tctgcgatat gtctcccctg acggaactcc
     1381 gggatgctct ggaaatcgtg gctctggtga aagagaagag ctgtttcaat ggaaagctgg
     1441 cgcttatagg atacagcatg ggcggaatgg tcgcttcctt gctagccgga agggagaggg
     1501 agttcgacgc cgtactcctc tggtcgcccg tcataatgaa cggcgagttc ttctccagag
     1561 aggagtacag cttttccagc gacgagaagt acaaagatat tcttgggtta aagctaggcc
     1621 acggctttca aaaggacggg ctcagcgtag atgccgccgc ggaactggcg aactaccgcg
     1681 gcccgctaat gataatacac gggtcggaag acgagtcggt accttacgaa ccagtattac
     1741 gttacgccag agataggggg attgtcttcc acacggtcaa gggggccaac cacaagtatc
     1801 agaggctcga ctggatagaa gagctcttcg aagtgtcgga aggctttctc tgcgatcgac
     1861 tactgtagcg ggtggtcggc cacccgcttt cctaccatca tctctctcat aatcagcaaa
     1921 gccctgtcta tcagttgctc gtcggtctca aaccagaatt catcgccgga aacgcctgta
     1981 acggccaatc cctgcctgga tagcgccgcc agtttttcga ccagcccgtg tctatgaagg
     2041 cctgtctcct tggcgatcga tcttatgttc atcctgcggt tcatgagagt tttcaaaagg
     2101 ttcatcgagc cttctacaga aagcactccc gctatttcga gagggtccat agcttccggt
     2161 agcatattct tcttcctcat cgtctcttcg cctacaattg agatcaccga gttcctttca
     2221 ggcaggttga cggtaataac ggagcctccg agaaaccatg aagaaaaaat atacaagtcc
     2281 gactcgctac tttcttcccg tctttcgata tccagcagct ctcttagaaa tggttggcca
     2341 tagagagaga agttgccatt gaaaagctct ctgaaggtcc gggagctttc ccgcaagatt
     2401 tccaagaact cgccgtcgaa gagttcccga caaatgaaat gcaaaagact cgaaaagctt
     2461 tctttcgctc cgttctggtc tctgaggatt tcgatggccg agtacctgct ggaattgccg
     2521 agttccaggt ttgaaaacac gtatttgagg gtttctcgct cgtcgagctc cagcagtctg
     2581 aaggcgagat ctctcgcttc gcagtcttcg tagtattgcg ggctgaaaaa ctttgccatc
     2641 aaactctgcg gtgaaagccg ttccagccac tccataaacg cttcacagga atcgatccct
     2701 tcgaagcaaa tataaggaag cgtcctcaac ccgaagaaac tatcccagct gaaaaaaacc
     2761 tcaatcattt cgaagagttc cgcgggaatg gatactctgg attttataac ccatcgtgca
     2821 agatggtcgg cagccagagt atctcgatcg atcattttct catggttgtt gagccgaaaa
     2881 agcgctgcga gcacggagac ggcctcgttg ttcaaaaccc taacatcagc catcgatagc
     2941 ctctccaaag atctccttaa cagaatctcc aacggcctta accaaaaagg ctttgttcag
     3001 gttgaaaagc cttctgtctc cttccacgct gtcttcaatg acgccatatc tcatcaacac
     3061 gctcaggtaa tcggttacct caacgttggt caatctagtg gaaagggcta tttccgtcac
     3121 tcccatggga gcttgagaga gtctttttag aatgcgcagg ttcacgtcgt ttgccaggat
     3181 ggtgaagaga tcgacccccg aagccattct atcgaccgga ggggttatcc tttcggggta
     3241 tctgtatccc acgacgtata tgtcctctct tctgtaaggg atggagccct tcaacaaaat
     3301 gatttcgtag aagaaggaga ccgctatggt aatggatccg gttgaaggaa cgccactcct
     3361 ggcagaactc ccggtgagcc attgaacgta gttttttccg tacttctcga aaagagcgtg
     3421 cagcttcgat cgctgttttt cgacctcatg
//
LOCUS       DAOOLY010000262         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_33312_length_3434_cov_8.495117, whole genome shotgun sequence.
ACCESSION   DAOOLY010000262 DAOOLY010000000
VERSION     DAOOLY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33312_length_3434_cov_8.495117"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLD15_10710"
     CDS             <1..836
                     /locus_tag="PLD15_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64424.1"
                     /translation="LNADDIAGIRALYAAGGGGDTPPPPPDVSPTTLYLLDNRFAVTL
                     TWRNQFNNTTGAGKAITFSNFVGFFYFENDPRALEILVKIVDYDGRILVFFSELGIIQ
                     FELNVTDRITGKSKTYRNTAGDCGAIDPDFHLSDGKTALMTGGRKATIRDLGITRAPK
                     GQCVPDDRTLCLLGDRFSVELPFWRNQFDESSGFGKPLPMGGLAGGFHFYNDPRDLEI
                     LFKIYEFPDRILVFYGTLSIFGYTIQMTDETTGRTTEFSNPEGTFCGGFINDLLAGPP
                     F"
     gene            952..2229
                     /locus_tag="PLD15_10715"
     CDS             952..2229
                     /locus_tag="PLD15_10715"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB64425.1"
                     /translation="MAALHRDDLPTGLSRLQRLKRVFRAFFIRMERGPSREEATRYRP
                     RDPEVEASRLLAAAQAYAGARSGAEHDWLFRKPFDPTPGNASFYTEMYQVLNLLEAMR
                     IAPGGLVLEVGCGPGWVTEILVLLGFRVVAVEPAAEMVAIARDRIAAARAKHHLAAGD
                     DVTFLCTSLETAELEAETFDAVLFHEALHHVIDERLGLGNCFSALRPGGVLGVSEWAW
                     RPGDGTLEAQLEAEMRRFGTLESPFYPPYLDRTLAAAGFVDLRRYHAINGFFPADAGE
                     TSIERLAQGPAASTNNLTVHKPGFPASPDLEAPLSAAITLLAVERSAESGMLRIAARI
                     ANTSPTYWLSRRRRFGYVTIALRAPDAGTFIEAESRHLLPTDLPPGAALEIDLRFRLP
                     EALDHLPWCLELVNEGICWFSERGVLPTRVEIS"
     gene            2408..3301
                     /locus_tag="PLD15_10720"
     CDS             2408..3301
                     /locus_tag="PLD15_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64426.1"
                     /translation="MLLLTAIDQRAQGEATINCPNGTIDILDWMTLDSQHRSTKHMAG
                     PGSSTLYTTVWDDKYFWVKTPSGDTMDINLFDESFVYMWYTENEWHNPYDFRKHTYDW
                     NMPMAPRCATGGYPGSTMTISDSSYSTYKNCELDSIRNVERVVFEVWGPYTAGKPGLE
                     SWRQPIGGDIPNTTPVYVISYRYGCNRNYTNCGDKEEYVLAQEYGLVRWQHFELVNGQ
                     YQLNGPGMTVNTLMSGTAVPFAPCMGQSRVAGESAPCEAWQSSVRRNLGPGATIEYRG
                     PETPHPFANRFSPENTILLTD"
BASE COUNT          674 a         1102 c         1014 g          644 t
ORIGIN      
        1 gcctgaacgc cgacgacatc gccggcatcc gggccctcta cgccgccggc ggcggtggtg
       61 acacgccgcc gccaccgccg gacgtctcgc ccaccaccct gtacctcctc gacaaccgat
      121 tcgcggtgac gctgacctgg agaaaccagt tcaacaacac taccggcgcg gggaaggcga
      181 tcaccttcag taacttcgtg gggttcttct atttcgagaa cgatcctcgt gcgctcgaga
      241 tcctggtgaa gatcgtggac tacgacgggc gcatcctcgt cttcttcagc gagctaggga
      301 tcatccagtt cgagctaaat gtcaccgacc ggatcaccgg aaaatcgaag acttatcgga
      361 atacggcggg ggattgcggc gcgatcgatc cggacttcca cttgagcgac ggcaagaccg
      421 cactgatgac cggcggcagg aaggcgacga tccgggatct cggaatcacc cgtgccccca
      481 agggccagtg cgtgcccgac gacaggaccc tctgtctgct gggtgatcgg ttttccgtcg
      541 agcttccctt ctggcgcaat cagttcgacg agtcttcagg gttcgggaag cctctcccga
      601 tgggcggcct cgctggcggg ttccacttct acaacgaccc tcgagacctc gagatcctct
      661 tcaagatcta cgagtttccg gatcggatcc tcgtcttcta cggtactctg tcgatcttcg
      721 gttacaccat ccaaatgacc gacgagacga ccggccgtac gaccgagttc tcgaaccccg
      781 aggggacgtt ctgcggcggc ttcatcaacg acctgctcgc cggcccaccg ttctagctca
      841 ggtccttcta tcgaggaatc tgttctctcg ccgcagacgc ctctcgcaga ggcattcggt
      901 cgcgcactcc ggttcggggc tcgccgctcc gtcatttgat atcctcttca cgtggcagct
      961 ctccaccgcg acgatcttcc cacggggctc agccgtctcc aacgcctgaa gcgtgtgttc
     1021 cgagccttct tcatacggat ggaacggggg ccgtcccggg aagaggcaac ccgctaccgg
     1081 ccccgagacc cggaagtcga ggcaagccgc ctgctcgccg ccgcgcaggc gtacgccggc
     1141 gctcgttccg gtgccgagca cgactggctc ttccggaagc cgttcgatcc gacaccgggg
     1201 aacgcgtcgt tctacacgga gatgtaccag gtcctcaacc tcctcgaggc aatgcggatc
     1261 gcacccggcg gactggtcct cgaggtcggg tgcgggccgg gctgggtgac ggagatcctg
     1321 gtcctcctcg ggttccgggt cgtcgccgtc gaaccggccg ccgaaatggt ggcgatcgcc
     1381 cgggatcgga tcgccgcggc ccgtgcgaaa catcacctcg cggcgggcga cgatgtcacc
     1441 ttcctctgca cctccctcga gaccgcggag ctcgaagcgg aaaccttcga cgccgtgctc
     1501 ttccacgagg cgctccacca cgtgatcgac gaacgtctcg ggctcggcaa ctgtttctcc
     1561 gcgctccgcc ccggaggcgt gctcggcgtc tcggagtggg cctggcggcc cggagatggc
     1621 acactcgaag cccagctcga agccgagatg cggcgcttcg gcacgctcga gagccccttc
     1681 tacccgccgt atctcgaccg cacgctggcg gcggccggtt tcgtggatct ccgccggtac
     1741 catgccatca acggcttctt ccccgcggac gcgggcgaaa cctcgatcga acgcctggcc
     1801 cagggaccgg cggcctcgac caacaacctc accgtgcaca aacccggctt ccctgcgagc
     1861 cccgacctcg aggcgccgct ctcggcggcg atcacgctcc tcgccgtcga gcgatccgcg
     1921 gagagcggca tgctgcggat cgccgcccgg atcgcgaaca ccagccccac ctactggctc
     1981 agccgccgcc gccgcttcgg atacgtgacg atcgccctgc gagcgccgga cgccggcacc
     2041 ttcatcgaag cggagtcgcg gcaccttctc ccaaccgact tgccacccgg cgcggctctc
     2101 gagatcgacc tgcgttttcg ccttccggaa gctctcgacc acctgccctg gtgtctggag
     2161 ctcgtgaacg agggaatctg ctggttttcc gagcgcggcg ttcttccaac tcgagtcgaa
     2221 atctcctgag acgaacgaaa ccggttgtga caaagttcac tgacaacgtc cacgaaaagg
     2281 gggagaattt tcctggttag gattggtccc aattgagggg ccaaccgaca caccggtgag
     2341 ggcgaaaaac tcacacaggg caaaggagga ggacatgcgt tccttactgc gagttcttgt
     2401 tgccatcatg ctgcttctta ctgcaatcga tcagcgggcc caaggtgagg ccacgatcaa
     2461 ctgtccaaac gggacgatag acattctaga ttggatgacc ctggattccc agcatcgatc
     2521 gacgaagcac atggcggggc ccggttcgag tacgctctac acgacggttt gggatgacaa
     2581 gtatttctgg gtcaagacgc caagcggcga cacgatggat atcaacctct tcgacgagag
     2641 tttcgtgtat atgtggtaca cggagaatga gtggcataat ccgtacgact ttcgaaagca
     2701 tacctacgac tggaacatgc cgatggcccc gcgatgtgcg acgggaggtt atccaggctc
     2761 cacgatgacg atttcagact catcttactc tacttataaa aactgtgagc tagattcgat
     2821 cagaaatgta gaacgggtcg tatttgaggt ttggggacca tataccgcgg gaaagccagg
     2881 cctggagtct tggcgtcaac ccatcggggg tgatatcccc aataccactc ctgtgtacgt
     2941 aatctcgtac cgctacggtt gcaatagaaa ttacacgaac tgtggtgaca aagaagaata
     3001 cgtactggct caggagtatg gtctcgtccg ctggcagcac ttcgagcttg ttaacggcca
     3061 gtaccaactg aatgggcccg gcatgacggt caatacgctt atgagcggaa ctgcggtacc
     3121 tttcgcgcca tgtatgggtc aatcacgtgt tgcaggtgag agcgcgccat gcgaggcatg
     3181 gcagagctct gtccgcagaa acctcggtcc cggtgcgacc atcgaatacc ggggacctga
     3241 aacaccgcac ccgtttgcca atcggttctc gcccgaaaac acgatactgc tgaccgacta
     3301 aactggaata gggaaagata gagaagaggc ggaagcctcg tcgcccgtcg cctcccgcca
     3361 gcgggcggcg aggtggccag cgaggaccca ctcggggtcg agggagatgc cgaagcgatc
     3421 gcgcacggcg tggt
//
LOCUS       DAOOLY010000263         3399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_33785_length_3399_cov_3.318481, whole genome shotgun sequence.
ACCESSION   DAOOLY010000263 DAOOLY010000000
VERSION     DAOOLY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33785_length_3399_cov_3.318481"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1494)
                     /gene="rpoD"
                     /locus_tag="PLD15_10725"
     CDS             complement(199..1494)
                     /gene="rpoD"
                     /locus_tag="PLD15_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPB64427.1"
                     /translation="MEKNKKKTKSEVEDLEEKIQSLAEKVIDDDDEETGKEESSNEES
                     GENGENGEREVPVTKEEIEKRIKKLLKQGKKKGYITYEDIDGAFPPDYEGFDSTLVES
                     IYEEFEKNKISIVEKEPVEEDDEIDNESSEELVSILETSPEMYDNISLKDPIKMYLKE
                     IGKISLLTPSRERELARRAQKGDPKAKEELITANLRLVVSIAKRYIGRGLTFLDLIQE
                     GNIGLIKAVEKFDWKKGYKFSTYATWWIRQAITRAIADQARTIRVPVHMVETINKLNK
                     VIREHLQDYGEYPTVEELGRITGKTPEKIEEILAASKETVSLESPISGDEESTMGDFI
                     HDDSLEQPEEAAMKMLLREQIDQILDTLSPREAMVLKMRYGLLDGKTKTLEEVGQFFN
                     VTRERIRQIEVKALRKLRHPSRSKQLKALLGMLNSSKRE"
     gene            complement(1496..3208)
                     /gene="dnaG"
                     /locus_tag="PLD15_10730"
     CDS             complement(1496..3208)
                     /gene="dnaG"
                     /locus_tag="PLD15_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB64428.1"
                     /translation="MFTKEELDEIKKAINIVDFIGRYVNLQKAGSSYRGLCPFHNDND
                     PSFYVHPQRGFYHCFGCGEKGDLISFYQKIESISFSEAIRRLADLAGIQIKIDNGESE
                     YDRYTSVLTKLSGLYSRELFENRNLTILEYLVNERKISESTIREFQLGYSPDDGTFGA
                     VVVSKLSVDEKTLINMGVAYRRGGSLKDRFEGRLIVPISNESGRVVGFGGRLASGDQG
                     PKYINSPETRYFQKNRLLFNLSRARGVIKELDYAVIVEGYFDAMALYQAGIVNVTGLL
                     GTALTEGHLRILGNYTKNLLFFLDSDSAGQTASLRSIDLAERMDFSTAVAMVKGAKDP
                     AELFVLKGSQGVNDALSMSIPGSIFRVEFYSRKLDLSLPQAKKHLIEYLKPYVESFSS
                     SGNLSAREMTFRALSEKTGFSEDELVRFTKKESRELDRLDHRTTITLRNKDILRVYLQ
                     YPPLRSRAARQLELLEPKKEFRELLRGMKKGLELEELLDLVDGEFGRELIELASSCID
                     QGTALRIIDSTGEYMNRRLVEEQIAEIDRRLSTLEDEEAKAALLIRRIELRRLLDKRK
                     RGGD"
     gene            complement(3276..>3399)
                     /gene="rpsI"
                     /locus_tag="PLD15_10735"
     CDS             complement(3276..>3399)
                     /gene="rpsI"
                     /locus_tag="PLD15_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB64429.1"
                     /translation="SPDLKGLLRQNGLLSRDSREVERKKYGLKKARRAPQFSKR"
BASE COUNT          715 a          939 c          736 g         1009 t
ORIGIN      
        1 ggcgctcgta tcaccgcttt tcgccattct taagaggtca atctgagaaa gaatacgggg
       61 actggttgtg actggtctag cgatcttttt ttcagccaag ttctcacgcc cctttttacc
      121 ggtgaataat tataaacaca tcctcttttc ccgttggtga tacttaaatt aaggcgctgt
      181 aatatatggg gagaaaattt attccctctt ggaactgtta agcataccca aaagcgcctt
      241 cagttgctta cttctagagg gatggcgcaa ttttctgagg gccttcacct ctatctggcg
      301 aatcctctct cgtgtaacat tgaagaactg gccgacctct tccagggtct tcgttttgcc
      361 gtccagcaat ccgtatcgca tcttcaaaac catggcctcc ctcggggaga gcgtatcgag
      421 tatctggtcg atctgctctc tgagcagcat tttcatcgcg gcctcttcgg gctgctcaag
      481 ggaatcatcg tgtatgaagt cgcccatggt ggattcctca tcgccactta taggagattc
      541 cagagagacg gtctccttag aagctgccag aatctcttct atcttttccg gagtctttcc
      601 ggtgattctt cccagttcct ccacggtcgg gtattcacca taatcctgta ggtgctctct
      661 gatcaccttg ttcagcttgt tgatggtctc gaccatatgc acgggcaccc ttatagtcct
      721 tgcctgatcg gcgatcgctc tggtgatagc ctgtctgatc caccatgtgg cataggtgct
      781 gaacttgtaa cccttcttcc agtcgaactt ctccacagcc tttatgagac ctatgttgcc
      841 ctcctgtatc agatctagaa aagtcaggcc gcgacctatg taccttttgg cgatcgatac
      901 aacgagtctc aggttggccg ttattagttc ctctttcgcc ttaggatcgc ctttctgggc
      961 ccttctggct agctctctct ccctgctggg agtgaggagc gatatctttc ctatctcctt
     1021 gagatacatc ttgatcggat ccttcagtga tatattgtcg tacatctcgg gtgaggtctc
     1081 aagaatcgat acgagctctt cgctgctctc attgtctatt tcatcgtctt cctccactgg
     1141 ttccttttcg acgatgctga tcttgttctt ttcgaactcc tcgtagattg actccacaag
     1201 ggtcgaatcg aacccttcat aatcgggagg gaaagcgccg tcaatatctt cgtacgtgat
     1261 gtaacccttc ttcttgccct gcttgagaag cttctttatt ctcttttcaa tctcttcttt
     1321 ggtaacaggt acttctctct ctccgttctc tccgttttcc ccgctttctt cgttactgct
     1381 ctcttcttta cccgtttcct cgtcgtcgtc atcgatcact ttttccgcga gagactggat
     1441 cttctcctcc agatcttcaa cctcagactt ggttttcttt ttgttctttt ccatttcagt
     1501 cgccacccct ctttctttta tcgagcagtc tccgtagctc tatcctcctg atcaacaagg
     1561 ctgcctttgc ctcctcgtct tccagtgtag ataatcgccg gtcgatttcg gcgatctgtt
     1621 cttcaaccaa tcttctattc atgtattccc cggtgctgtc tataattctg agagccgtac
     1681 cttggtctat gcaactggat gccagttcta tcagctctct tccaaactcc ccatcaacca
     1741 gatcaagaag ctcctcgagc tccaatccct tcttcatgcc tctcaaaagc tctctgaact
     1801 ccttcttcgg ttcaaggagc tctaactgcc ttgccgcacg gctccgcaag ggcgggtact
     1861 ggagatagac acggaggata tctttgtttc tgagagttat agttgtcctg tggtccaatc
     1921 tatccagctc cctggattct tttttggtga acctcaccag ttcgtcttcg gaaaaaccgg
     1981 tcttctccga aagcgcccta aaggtcatct ctctggcaga gagattgccg ctcgaactga
     2041 aagactctac gtatggttta agatattcga tcagatgttt cttcgcctgt ggaagcgaaa
     2101 gatcgagctt cctcgagtag aactcaaccc tgaagatcga cccgggaatc gacatcgaaa
     2161 gggcatcgtt gaccccctga gaacccttca aaacgaagag ttcggccgga tctttcgcac
     2221 ccttcaccat cgccacagcc gttgagaaat ccattctttc ggctaggtct atcgaacgca
     2281 acgatgccgt ctgaccagcc gaatcggaat cgagaaagaa aaggagattc ttcgtataat
     2341 tcccgagaat gcgcaggtgc ccttcggtca gcgcagtgcc caggaggccg gttacattga
     2401 ctatcccggc ctggtaaagc gccatggcgt cgaaataacc ttccactatc actgcatagt
     2461 ctagctcctt gatcacaccc ctagccctgg aaagattgaa cagcaaacgg tttttctgga
     2521 agtatctggt ttccggagag ttgatgtatt tcggtccctg atcgccagaa gcgagcctcc
     2581 ctccgaaacc aactacacgt cccgattcgt tgctgatcgg cactatgagc cttccttcga
     2641 acctatcctt tagcgaacct ccccgccggt acgcaactcc catattgatc agagtctttt
     2701 cgtccaccga gagtttcgac accacaacag ctccgaaggt tccgtcgtcg ggcgaatacc
     2761 ccagctggaa ctctctgatc gtgctctcgg agattttgcg ctcgttgacc agatactcca
     2821 ggattgtcaa attgcggttt tcgaagagct cgcgcgaata cagcccggaa agtttggtca
     2881 atacggaagt atagcggtcg tattcggatt caccgttatc gatcttgatc tgtattccgg
     2941 caaggtccgc aagcctcctg atcgcttccg agaaggatat actctctatc ttctggtaga
     3001 aagagatcag atcgcccttc tcgccgcaac cgaagcagtg atagaagcct ctctgggggt
     3061 ggacgtaaaa tgaaggatcg ttgtcgttat gaaatgggca cagtcccctg taagagcttc
     3121 cggccttctg caggttcacg taccgaccta tgaaatcgac gatgtttatt gcctttttga
     3181 tctcgtcgag ttcttctttg gtgaacactg aatcaccacc ggcaaaaaat gggcccatac
     3241 tttctcaggt acgggcccag cttatgtaag agtaattaac gcttggagaa ttgaggtgct
     3301 cttcttgctt tcttcaaacc atatttcttt ctttcgactt ctctggaatc ccttgataga
     3361 agcccgttct gtcttagaag acccttcaga tctgggctg
//
LOCUS       DAOOLY010000264         3373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_34120_length_3373_cov_4.670283, whole genome shotgun sequence.
ACCESSION   DAOOLY010000264 DAOOLY010000000
VERSION     DAOOLY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34120_length_3373_cov_4.670283"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..933)
                     /locus_tag="PLD15_10740"
     CDS             complement(124..933)
                     /locus_tag="PLD15_10740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB64430.1"
                     /translation="MNRNDKAFPFDRIAQTYSQFRYPNEKLVEHIFNRVRYADNILEA
                     GCGPADYLSILSELLNARGYGFDISQNMIEEAKRKNSSLEFIVSDAQEPFPYGDGSFD
                     FVFNINLVHYLSELDNLFRESYRVLMYDGSALTVTDSCQDIEDRTLTHYFPEVLEIDR
                     KRYPGNSAIMSAMKQAGFEGIYTTKVKTTFEFKSQHYEQYRERAYSALRLISDEAFER
                     GMKRVENDMAGGLVIGKKSYTQIWGIKVEKREFDLPTIFSTGYHEGGNNSF"
     gene            1123..2010
                     /gene="rapZ"
                     /locus_tag="PLD15_10745"
     CDS             1123..2010
                     /gene="rapZ"
                     /locus_tag="PLD15_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPB64431.1"
                     /translation="MSSSIVIVTGMSGAGKSSAIDVLEDFGFFCMDNVPPTMIEQLVG
                     MINEHSVEKSAFVMDIRTAKKFGGMDKLRKSLLALKDRGVNILSIFLDADDDVLYYRY
                     QKTRRAHPLQEEQELEKAIQKERSIMNQLKDHCDYILNTSTMDMKEMKEAIISIIRNR
                     GYKLPPMKVEIESFSYNQGIPHDANLIFDVRFLPNPYYHENLTELTGMDREVQEYLET
                     FPGLEKYFQAVLNLCKMTIEGFAGSGRNSVKIAVGCTGGRHRSVYIAERLYEALKSPD
                     LKLSIDHREQKKHRNNSEI"
     gene            2194..3183
                     /locus_tag="PLD15_10750"
     CDS             2194..3183
                     /locus_tag="PLD15_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HPB64432.1"
                     /translation="MKRTEILYLFLLFSVTVAVSSGFLVLKGDFSAIAVTEKAIRRGE
                     SFSLEVPVSGLERPVLAYSRGASLLDFQAGEGTVSYLFTADDPDAVIVVEYDGLFKAR
                     KVVSLDFEGFVDENSDGFPDMLVLDSNDAWCFRAWFVNIAAYQAIQFSGRWSDQERDC
                     SGLIRFAAREALKVHDDNWFGETGIDTRLWFEKTGIDLTSLPDVTRYNYPAIPIVGPK
                     LFIDSAGQFTYFADAYNLVRSNMVYRGRDIASARPGDIIFFHHPSPSTFHSMIYTGDG
                     LIYHTGPLSPGNSGSLKVWSLDDYMRMMPYQWLPINNNEYFLGVYSFKWLPRM"
BASE COUNT          844 a          811 c          838 g          880 t
ORIGIN      
        1 tccagcgaac ctgaggacat gtcaagattg aaacctattc ccacgttgaa cgcgatagaa
       61 aacaccatgg tcgatagtac cagtaccgga attagcagca actttttcac ttcgaaaccc
      121 ccttcaaaat gagttgtttc cgccttcgtg gtaacccgtc gagaagatcg taggaagatc
      181 gaactccctt ttctcgacct ttattcccca gatttgcgtg tagcttttct tacctatcac
      241 aagccctccg gccatgtcgt tctcgactct cttcataccc ctctcgaaag cctcgtcgga
      301 gatcagtctg agggcggagt aagccctttc cctgtactgt tcgtaatgct ggcttttgaa
      361 ctcgaaagtg gtcttgacct tggtcgtgta gatcccctcg aagcccgcct gtttcatggc
      421 gctcattatg gccgagttgc cagggtatct ctttctgtcg atttccagca cctcggggaa
      481 gtaatgtgtc agtgttctgt cttcgatatc ctgacaggag tccgtgacgg ttagggcgct
      541 gccgtcgtac ataaggactc tgtaactctc tctgaagagg ttgtccagtt cggagaggta
      601 gtggaccagg tttatattga agacgaagtc gaaactgccg tcaccgtaag gaaacggttc
      661 ctgcgcatcg ctaacgataa actcaaggct cgagttcttt cttttcgctt cttctatcat
      721 gttttgactt atgtcgaagc cgtaaccgcg ggcgttgaga agctcggaaa gtatcgaaag
      781 gtaatctgca ggtccgcatc ccgcttctag aatgttatcg gcgtaacgca ctctgttgaa
      841 gatatgctcg accaattttt cgttgggata tctgaactgc gaatatgtct gcgctatccg
      901 gtcgaacgga aaagctttat cgtttctgtt cattcaattc acctctagct tatagtgtaa
      961 cagaattgcg ggttcaaaga attggaattg gagtgtcttt ccgaccacga ttctgatctc
     1021 tatctcctta atcattgtat aatcaacacg agggatcggc tctctttttc attcttagta
     1081 tatttctgtt tgtttataac attatccagg ggagtggtat gaatgtcttc aagcattgtt
     1141 atcgtaaccg gcatgtcggg cgccggcaag tcttcggcca tagacgtgct ggaggatttc
     1201 ggcttcttct gcatggataa cgttcccccg actatgatcg agcaactcgt aggtatgata
     1261 aacgaacact cggttgaaaa aagcgccttc gtcatggata taaggacggc gaaaaagttc
     1321 ggggggatgg ataagcttcg caaaagcctg cttgctttga aagatagggg ggtcaacata
     1381 ctctctatct ttctcgacgc ggatgacgac gtactgtatt accggtacca aaaaaccagg
     1441 agggctcatc ccctccagga ggaacaggaa ctggaaaagg cgatacaaaa agagcggagt
     1501 atcatgaatc agttgaaaga ccactgcgat tatattctta acacctcaac catggacatg
     1561 aaggaaatga aggaagccat aatctccatc atccgtaacc gcggctacaa actgccacct
     1621 atgaaggtcg agatcgaaag cttctcttac aaccagggca taccgcacga cgcaaaccta
     1681 atcttcgatg tcaggttcct tcccaatccc tactaccacg aaaacctcac ggagctgacc
     1741 ggaatggaca gggaggtcca ggaatatctg gagacctttc ccggactcga aaagtacttc
     1801 caagccgttc tcaacctctg taaaatgacc atcgaaggct tcgccggaag cgggagaaac
     1861 tcggtcaaaa tagcggtggg atgcaccggc ggccgacaca ggtcagtata tatcgccgaa
     1921 agactctacg aggctctgaa atcaccggac ctgaaactct cgatagacca cagagaacag
     1981 aaaaaacacc gtaacaacag cgagatttaa aggatagtgg gaaaaggtca tcgggagttc
     2041 tacagcgttc ttagacgtta gagggctacc agagactaga ggtgacaggt taaggaggat
     2101 cttcgccgga attgttgtga accaatcggc gcggtgaaag tcttataaac tgtgaagatt
     2161 tttgattatc gcgatttctg aggggagata gcaatgaaac gtaccgagat tctatatctc
     2221 tttttgcttt tttccgtcac agtcgccgtt tcttcgggtt ttctcgttct gaagggcgat
     2281 ttcagcgcta ttgcagtcac ggaaaaggct attcgacgcg gagagagttt ttctctggag
     2341 gttcccgtat ccggtctgga gcgtccggtg ctggcttatt ctcgtggagc cagccttctc
     2401 gattttcaag ccggagaggg gacggtcagc tacctcttca cggccgacga tcccgacgcc
     2461 gtgatagtgg tcgaatatga cggcctcttc aaggccagaa aagtcgtttc gctggacttc
     2521 gaaggtttcg tggatgagaa ttccgacggc ttcccggata tgctagttct cgattcgaac
     2581 gacgcctggt gcttcagggc atggttcgta aacatcgcgg cctatcaggc catacagttc
     2641 tctgggcggt ggagcgatca ggagagagac tgctcgggcc ttataagatt cgcggcgaga
     2701 gaggcgttga aggttcacga cgataactgg ttcggggaga cgggaataga cacgcgtctt
     2761 tggtttgaaa agaccggtat agacctgacg agccttcccg atgttaccag gtacaactat
     2821 cctgccattc cgatcgtggg gccgaagctt ttcatagatt cggcagggca attcacttac
     2881 tttgccgatg cttacaacct cgtcaggagt aatatggtat ataggggcag agacatcgcg
     2941 tcggccagac ccggagatat tatctttttc catcacccct caccttctac ttttcactcc
     3001 atgatataca ccggcgatgg attgatctac cacacgggtc ccctttcacc tggcaacagc
     3061 ggctctttga aggtctggag tttggatgac tatatgagaa tgatgcctta tcaatggttg
     3121 cctataaaca acaacgaata ctttcttggg gtttacagct tcaagtggtt accccggatg
     3181 tgatggaggg tgaaaatgaa gagaatcatc gttctcctgc tcctgttagt ttccgcgatc
     3241 accgcggtac aggcgtttca gatccagcag tatggctaca gattcgatgg ctttttcatc
     3301 gaaatctact cactggacaa tcttccgacg atctataccg acaaaacaga tggctacatc
     3361 gacatcaagg tgt
//
LOCUS       DAOOLY010000265         3357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_34347_length_3357_cov_4.021805, whole genome shotgun sequence.
ACCESSION   DAOOLY010000265 DAOOLY010000000
VERSION     DAOOLY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34347_length_3357_cov_4.021805"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLD15_10755"
     CDS             <1..385
                     /locus_tag="PLD15_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64433.1"
                     /translation="KYYPDDPRPQNALKAAREWLSGRIKLPEAKGRILQCHAAAREAE
                     NNPVARTAARAIGQSASTVHSARHCIGLALYGALAVAYDALGTDAPREQLERLVAEEC
                     ERMLDALREVSIENEPNPAKIDWKC"
     gene            complement(711..3185)
                     /locus_tag="PLD15_10760"
     CDS             complement(711..3185)
                     /locus_tag="PLD15_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPB64434.1"
                     /translation="MPAFFSVFAFAVPNPMNPFPYNLHQTAFGWTNTSQHNIWDWMFY
                     AYYIGYALIGLLLLYRWGAKSSAPVTKKNSRIVRVSIIAALFLGTITDVVLSSVFSEL
                     PQMAPAIMLIPIISIYHVLQKDSFGITKGIDKKTSYMILFVSVLAYIILSALQVLLPE
                     KSFAIASVVLDKSEIRGIFVQIQMFISIYLVLKGNRPGYIASLIINSISLVTAIGFLI
                     RNKSTSSLPGIISYTGVLVIISLIRAYKEKNDDYIKRINTQTVREKFYSSVFKQAPVG
                     IAIISGTEFTRNEEFDDLNINPAYERILCRTREELQNTDWTTITHPDDLATDLAYFDQ
                     FKKGKIDYYPREKRYIKPDGSVVWVDMLISRFASSNEKPGDHVCIITDITNRKNIEAT
                     LKYNSEHMLLTGLYNRGILEKTLESDALIPSSYKRALVSINLSAMHVLSLRYGNNYNQ
                     TLLRNIADSLKAYSNDKRVLFNTYEDRFVFYVKDYEDEKELTAFSERLLYTLSSYLYI
                     HGIAVGIGILQIDKSTAHDTDELLTKLVNTSEIAAKNNGSGSNILFHSTELDIQITRE
                     NEIGQEIREIAEGIKIDRLYLHFQPILDVATNKVCKFEALARLDSEKHGFVPPLEFIP
                     VAEKTNMLAPFGERIIILALRFLKKLKETGHDTIAVSINISIIQILENGFASRLLNMI
                     NDMHLNPENVEIELTESVFSIEREEINTVINELKAAGIKVLIDDFGTGYSSFARVSEL
                     DIDCLKIDKSFIDKLLVSKTEEAITGDIISMAHKLGHCVVAEGVEYEEQLSYLREHNC
                     DMIQGYLISRPLDEDSALNLLKNNKK"
BASE COUNT          933 a          749 c          738 g          937 t
ORIGIN      
        1 taagtattat ccagacgacc cgcgtccaca aaacgcattg aaagccgccc gggagtggtt
       61 gtcgggaagg ataaagttgc cggaggcgaa agggagaatc ctccagtgcc acgcggccgc
      121 cagagaagcc gaaaacaatc ccgttgcccg aaccgccgcg agagctatag gtcagagcgc
      181 atcgaccgtg cactcggcga ggcactgtat cggactggct ctatatggag cgttagcggt
      241 tgcgtacgat gcgcttggaa cggacgcgcc ccgggaacaa ctggagcggc tagtcgccga
      301 agaatgcgag cgtatgcttg acgcgctgcg tgaagtgtcg attgaaaacg agccaaaccc
      361 tgcgaaaatc gactggaagt gttgaacgac ggggatagag caccggttga actactcacc
      421 acctaagagg tgggagactc cttggtgtct gtttataaaa gcgatataaa tgataaccag
      481 caacaaagaa aacatatgaa ccctctcaat tcagatggtt ttttcacacg ataccttgcg
      541 gcatgtctgc cgatatgtag aaaccgacgc gccttgtttc gcaagaaact ctattcaact
      601 ttacagcatg gaaatgttta ccggaaatgc tttttgtcgc cacgataaac cgcaaggaaa
      661 gagagaatag ctcttgcaaa aacagatgtg gcatcgcggt gtccctgtat ctacttcttg
      721 ttgtttttca agaggttgag tgctgaatct tcatcaagtg gtctacttat cagatatccc
      781 tgtatcatgt cacagttgtg ttcccgcagg taactgagct gctcctcata ctcaacccct
      841 tcggcaacaa cacaatggcc cagcttatga gccatggata ttatatctcc ggtgatcgcc
      901 tcttccgtct tcgataccag caatttatca atgaagcttt tatcaatctt caagcaatcg
      961 atgtccagtt cacttacacg ggcaaaagag gaatagcctg tcccaaaatc atcgatcagt
     1021 actttgatcc cggcggcttt aagctcgtta ataacagtgt ttatttcttc tctctctatg
     1081 gagaataccg attcagtaag ttcaatttca acattctctg gattcaggtg catatcgttg
     1141 atcatgttca gcaacctact ggcaaatcca ttttccagta tctgaattat cgaaatgttg
     1201 atcgatacag ctatcgtatc gtggccagtt tccttcagtt tcttcagaaa acgaagagcc
     1261 aggataatga ttctttctcc aaacggagca agcatgttcg ttttttcggc caccggaatg
     1321 aactccaaag ggggaacaaa accatgtttt tcactgtcaa gcctggctaa agcttcgaat
     1381 ttacagacct tatttgtagc aacatcaaga atcggttgaa aatgcaaata gagtctgtca
     1441 atctttattc cctcggctat ttctcttatc tcctggccga tctcgttttc acgtgtaatc
     1501 tgaatatcca gttcagtact atggaacaaa atattagaac cactgccgtt gttttttgct
     1561 gctatttcac ttgtattcac aagttttgtc aacagctcgt cagtgtcgtg cgcagtcgat
     1621 ttatctatct gaagtatccc tatgcccaca gctattccgt ggatatacag ataagaagac
     1681 aatgtatata atagtctttc agagaaggca gtcagttctt tttcatcttc ataatctttc
     1741 acataaaaaa cgaatcgatc ctcgtacgta ttgaataaca cacgtttatc attactataa
     1801 gcctttagag aatcagcgat attcctgagc agagtctggt tgtaattatt cccataacga
     1861 agactcaaga catgcatggc agagagattg atactcacca aagcgcgctt gtatgacgag
     1921 ggtatgagtg cgtcgctttc cagtgttttt tccagtattc cacggttata aagtcctgtc
     1981 agcagcatgt gttcactgtt gtattttaag gttgcctcga tgttctttcg atttgtaata
     2041 tccgtaatga tgcaaacgtg gtcaccgggc ttttcatttg aactggcaaa acgagatata
     2101 agcatatcga cccaaacaac ggagccgtcc ggcttgatat agcgcttttc ccgcgggtaa
     2161 taatctatct tgcctttctt gaactgatcg aagtatgcca gatcggtagc aagatcgtcc
     2221 ggatgcgtta tcgtcgtcca atcggtgttt tgcagctctt ctctcgttcg acagagaatt
     2281 cgttcgtacg ccgggttaat attcaagtca tcgaattctt catttctcgt gaattcagtc
     2341 ccgcttataa tagctatccc aaccggggct tgtttaaata cactggaata gaatttctct
     2401 cggaccgtct gggtattaat tctcttgata tagtcatcat ttttctcctt ataagctctt
     2461 atcaaagaaa tgatcacaag gactccggta taagagatga ttcctggtaa agatgaggtc
     2521 gacttgttgc gaatcaggaa tccaattgca gttacgagac tgatggagtt aattatcaaa
     2581 gacgcgatat accccggcct gtttcctttc aataccaggt atatcgaaat gaacatctgt
     2641 atttgaacaa agattcctct gatttccgat ttgtcgagta cgacagacgc tatggcaaag
     2701 cttttctctg gaagcaacac ttgcagtgca gaaaggatta tgtatgcaag aacacttacg
     2761 aatagaatca tgtagctcgt ttttttgtct atacctttcg ttataccaaa gctgtctttt
     2821 tgcagaacat gatatatgga gatgatgggg atcagcataa tagccggggc catctgaggc
     2881 aactcagaaa atacgctgct caaaacgaca tcggtaatcg ttcctagaaa aagagccgcg
     2941 atgattgata ctctcacaat gcgggagttc ttttttgtta caggagctga tgattttgcg
     3001 ccccatctat acaaaagcag taaccctatg agcgcataac ctatgtaata ggcatagaac
     3061 atccagtccc agatattgtg ttgtgaagta ttggtccagc caaacgcggt ttggtgaaga
     3121 ttatatggga aagggttcat cggattggga acagcgaaag cgaaaacgga aaagaaggct
     3181 ggcaaataca gacataagta gaaccaccac tttctgagcg aaggggctct acccgttatg
     3241 atcaatatga agtggagtaa tatcgcgtaa actgtgctcc aaccgaccgc cgaaatacgc
     3301 cgaaagattt cgcatgtcgc agcgtcattg gcagtggttg acatcgccat gccagat
//
LOCUS       DAOOLY010000266         3353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_34398_length_3353_cov_4.063978, whole genome shotgun sequence.
ACCESSION   DAOOLY010000266 DAOOLY010000000
VERSION     DAOOLY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34398_length_3353_cov_4.063978"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLD15_10765"
     CDS             complement(<1..657)
                     /locus_tag="PLD15_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPB64435.1"
                     /translation="MLNYIAGQWYDENDSLVSALIPGVLNCESVYEVVRTYNGLPFAF
                     RKHFDRLKQSAGLMSIEVPFTCKELNSVILEGIDKNRLSQSGDFRIRIVLFGGGEGVL
                     PAVIYTQLPSVSKDIYELGVKIAISPYTKPSGAIVDPRLKIPGASWNIRTRKNLGDNY
                     DMIILNEKGNVCEGSFSNVFLLKDGQVITPDIGSGVLPGITRDNLIGLCSSIEINVME
                     R"
     gene            complement(623..1909)
                     /gene="mtaB"
                     /locus_tag="PLD15_10770"
     CDS             complement(623..1909)
                     /gene="mtaB"
                     /locus_tag="PLD15_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPB64436.1"
                     /translation="MKKTVSIFTFGCKLNQYESQAMAERLQRYDVRFSQEKSDLFVLN
                     SCTVTSEAERKLRQLFRRLLSLNPDSRIVVVGCYSELAGEELKKLGAHAVLGIQAKGE
                     IEKYVSSLLEDGSEGVEETSDGFLTVTSSLEGRTRAFLGIEDGCLNNCTYCRIRLARG
                     SRIISKPIDMVKNEFIGLVENGYREIVLTGINIGYYGFETGGDLEQLLRELNTVPGEW
                     RIRLGSLDPDRIDNELLNVILSTKRMARHLHLSLQSGSNRVLRLMKRKYEMKDYLKAV
                     ERARSMDSRFSFTTDVIAGFPGEEDFDLETTLQAIATIGFLKVHVFRYSNRPGTEAAS
                     MKDQVESSLKKKRALLVAGAAEESRRKYLSKHIGITNTVLVEKICHGDSVGYDEYYIP
                     HRIAGQYDGFVDSTAVNLEMHKEGSDAELYCRSVVR"
     gene            complement(1906..2358)
                     /locus_tag="PLD15_10775"
     CDS             complement(1906..2358)
                     /locus_tag="PLD15_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB64437.1"
                     /translation="MKVREAMIRDVSAIFEDETIEDFIVSCLRQNKSGLPVVDDEFKV
                     VGFLSESDVIRSALPSYFSLLQSASFIPDTHQFVLRLEKIKNDKVSLHMIKRVTCVKL
                     DDTVIYAADLLIKNNLKILPVVDEERRLIGIVNRIFLIHAATQGKIDR"
     gene            complement(2365..3324)
                     /locus_tag="PLD15_10780"
     CDS             complement(2365..3324)
                     /locus_tag="PLD15_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB64438.1"
                     /translation="MDVLTVTLNPALDREIVVENFTINDFHRVKNPSYSKMDPGGKGI
                     NVSVILSGIGVRNVAMGFLGGYIGKVVEEKLRTMSDLITTNFLYVEEETRENIAIVDP
                     IMDTITEINSSGPLIKPDDLRMFLRRFDVALSRVRHVVVSGSVPRGIENDIYSVICRK
                     TIEAGKFSFVEAIGPTFENAVDAGLVTVSRPDLRSRKILFGQTLVELRDYVDVAKYMI
                     SRGSRLSVLSYAIEGDVIATRDGVWLFRAKSHIEKSHLLGTGDAFMAGMVHSIINDER
                     DFFQAARKGMAAAIAEAGFISKELITPKDIEDNLESFEIRRLE"
BASE COUNT          769 a          897 c          752 g          935 t
ORIGIN      
        1 cctctccata acgttgatct cgatgctgct gcatagacct atcaggttgt cccgggttat
       61 cccgggtagt actccggaac cgatatcggg ggttataacc tggccatctt tcaagaggaa
      121 gacgttgctg aaagagcctt cacaaacgtt acccttttcg ttcagtatga tcatgtcgta
      181 attatcgccc agattctttc tggtcctgat gttccaactc gcccccggta tcttcagtcg
      241 cggatcgacg attgcccccg agggttttgt gtagggcgag atggcgatct tcactccaag
      301 ctcgtatatg tccttggaga ccgatggtag ctgagtgtaa attaccgcgg gcaacacacc
      361 ctctcccccg ccgaaaagga ctatcctgat cctgaaatcg ccgctttggg agagccggtt
      421 tttatctatt ccttccagga ttacggagtt caactccttg catgtgaagg gaacttctat
      481 ggacatcagc ccggccgatt gtttcaacct gtcgaaatgc tttctgaagg cgaacggaag
      541 tccgttgtac gttctcacta cttcgtaaac cgattcacag ttcagaactc ccggtatcag
      601 tgcactgact agggagtcgt tttcatcgta ccactgacct gcaatatagt tcagcatcgg
      661 aaccctcctt atgcatctcc agattgactg cggttgaatc gacgaaaccg tcgtactgac
      721 cggctatacg atggggaata taatattcat cgtaaccgac actatctccg tgacagatct
      781 tttcaacgag aactgtgttg gttatgccta tgtgcttgga taagtacttt ctcctcgatt
      841 cttcggctgc gccggcgact agaagcgctc tcttcttttt cagtgacgac tcgacctgat
      901 ctttcataga ggcggcttcc gtgcccggtc tgttcgaata cctgaaaacg tgtactttca
      961 agaagccgat agtagctatg gcctggagcg tcgtctctag atcgaaatcc tcttcccccg
     1021 ggaagccagc tatgacatct gtggtgaagg agaacctgga gtccatcgac ctcgccctct
     1081 ccaccgcctt cagatagtcc ttcatttcgt acttcctctt cataaggcgg agcaccctgt
     1141 tcgagccgct ctgaagagag agatgaagat gcctggccat acgtttggtg cttagtatga
     1201 cgttcaaaag ttcgttatcg atcctgtcgg gatctagaga gcccagtctg attctccact
     1261 cgcccggtac ggtgtttagt tctctcaaaa gttgctccag gtcaccgccc gtttcaaaac
     1321 cgtagtaacc tatatttatg ccggtcagta ctatctcgcg atatccgttt tctacaagcc
     1381 cgataaactc gttcttgacc atatcgatcg gcttgcttat tatcctgcta cctctggcca
     1441 ggcgtattct gcaataagtg cagttgttca ggcaaccatc ttcgattcca agaaaggctc
     1501 tcgttcgccc ctcgaggctg gaagtgactg tgagaaaacc gtcgctggtc tcttcaaccc
     1561 cttcgctccc gtcttccaga agactcgaga catacttctc gatctcgcct ttggcctgta
     1621 ttccaagaac ggcgtgggct ccgagcttct tcagctcttc acccgctagc tccgagtagc
     1681 acccgaccac aacgatcctg gagtcgggat tcaggctcaa gagacgtctg aaaagttgtc
     1741 tcagctttct ttcggcttct gaagtgactg tacaggagtt cagcacgaaa agatccgact
     1801 tctcttgcga gaaacgaacg tcgtaacgct gtagtctttc ggccatggct tgcgattcgt
     1861 actggttcaa tttgcagccg aaagtgaaaa tcgaaacggt tttcttcatc tatcgatctt
     1921 gccctgtgtt gccgcgtgaa tcaagaagat tctgttgact atccctataa gtcttctctc
     1981 ttcatcaacg acgggcaaaa tcttcaaatt gttctttata agaagatcag ctgcatagat
     2041 aactgtatcg tccagtttaa cgcatgtcac cctcttgatc atgtgcagcg atactttatc
     2101 gttctttatc ttttccaatc tcagcacgaa ctggtgggta tcggggatga acgaggccga
     2161 ctggaggaga ctgaagtaac ttggcaaagc gcttcttata acgtcgctct cgctcaagaa
     2221 gcctacgacc ttgaattcat catcgaccac tggcagacca gatttgttct gtctcaaaca
     2281 gcttacgatg aaatcttcga tagtttcgtc ttcaaatatc gcagagacat ccctgatcat
     2341 cgcttctcta actttcaccc tttatcactc cagcctgcga atctcgaaag attcgaggtt
     2401 atcctcgata tccttcggtg ttatgagctc cttgcttata aaaccagcct ccgctatggc
     2461 ggcggccatg cccttacggg ccgcctggaa gaagtctcgc tcgtcgttga tgatagagtg
     2521 gaccattcca gccatgaagg cgtctcctgt gccgagaaga tgggatttct caatatggct
     2581 ttttgctctg aaaagccata caccgtctct tgtcgctatc acgtcgccct ctatcgcata
     2641 ggagagaacc gaaagccttg aacccctgga gatcatgtat ttggccacat ctacgtaatc
     2701 tcggagctcg accagagtct ggccgaaaag tatcttcctg cttctgagat caggcctaga
     2761 aacagttacc agaccggcat ccacggcatt ctcgaaggtt gggcctattg cttcgacgaa
     2821 ggagaatttg ccggcctcta tcgttttcct gcatatcacc gaatagatgt cgttttctat
     2881 gcccctggga acgcttccgg aaaccaccac gtggcgcacc ctagaaaggg ctacgtcgaa
     2941 ccttctgagg aacattctga ggtcgtcggg ctttattaga ggaccggagg agttgatctc
     3001 ggttatagta tccattatcg gatcgacgat ggctatattt tctctggtct cttcttcaac
     3061 gtaaaggaag ttggtggtta tcaagtcgct catggttctc aatttctctt ccacgacctt
     3121 gcctatataa ccacccaaaa aacccatcgc tacgtttctc acacctattc ccgagagaat
     3181 gaccgaaacg tttattccct ttccgcccgg gtccatcttt gaataagaag ggtttttgac
     3241 ccggtgaaag tcgttaatcg tgaaattctc aacaactatc tccctgtcaa gggcagggtt
     3301 cagagtgacg gttaggacgt ccacaattca cctccgataa atttttaatc agc
//
LOCUS       DAOOLY010000267         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_34879_length_3318_cov_6.559914, whole genome shotgun sequence.
ACCESSION   DAOOLY010000267 DAOOLY010000000
VERSION     DAOOLY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34879_length_3318_cov_6.559914"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..819)
                     /gene="surE"
                     /locus_tag="PLD15_10785"
     CDS             complement(37..819)
                     /gene="surE"
                     /locus_tag="PLD15_10785"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB64439.1"
                     /translation="MNILITNDDGIMSPGIIELADRLSERHNVLVVAPDVERSATGHA
                     ITIRTPLWAKEVKVGNRMIGYAINGTPADCVKLGLLAISEIKRDLVISGINKGQNMGI
                     DVLYSGTVSGALEGAVMDTPSIAVSSSDWSNPIYSTAADFMLEFLETFDVKEMPPFTA
                     LNINVPSVAREDLKGLRITRQSRRRYKDYFEKRKDPYGNNYYWIFGEVVEDECSLDSD
                     FCAVKENYVSVTPLHAIMTDKDYFEELKELSKGWGKMSTTPG"
     gene            916..1578
                     /locus_tag="PLD15_10790"
     CDS             916..1578
                     /locus_tag="PLD15_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB64440.1"
                     /translation="MERKLKIAQLFERHPELEEIYGLARVAFEASRDPAHDLYHVVRV
                     TENAVEIALGEDESLETAALAALLHDIKRPSEDIDGLDHAAEGGKYSLKLLLSMGYTY
                     DIASEVALSIRDHRFSSGREPGSATGKILQDADRLDAIGAIAIARVFSYSGKVGRPLH
                     SPDLSPRDRYEGFSASAINHFYEKILKITPESFWTGTGRELARERYEFTVAFVERFLE
                     EW"
     gene            complement(1583..1852)
                     /locus_tag="PLD15_10795"
     CDS             complement(1583..1852)
                     /locus_tag="PLD15_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPB64441.1"
                     /translation="MRTVRVRVYGRVQGVGFRYYTLHTARALGITGYVRNEYDGSVEI
                     VGAGEEASIDEFVQRVDRGPVRAHISKCLVEELPEQHFTDFEVRY"
     gene            complement(1849..2490)
                     /locus_tag="PLD15_10800"
     CDS             complement(1849..2490)
                     /locus_tag="PLD15_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPB64442.1"
                     /translation="MRSSLLNWKYDKLAGKLIENFAKRSIEAVYLTSREQVVPRLEEL
                     IPAGSTVASGGSVTLQETGARELLKSGRYNYLDREAVPDGEEREEILRKAFWADFYLC
                     SANAITLDGQILLLDGNCNRAAAVLFGPKRVILVVGMNKVVGDIDAALERIRFIAPMN
                     AKRLNLHTPCTATGFCMDCTSNERICGTYVTVSDSSRRKGRYTVILVGEELGL"
     gene            complement(2518..2868)
                     /locus_tag="PLD15_10805"
     CDS             complement(2518..2868)
                     /locus_tag="PLD15_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64443.1"
                     /translation="MAKEVLILAFEGKKPCFLHALLNALDMHEKGFDVSVVLEGQSPS
                     LLKEITLESDPLFPLYSKAKSKGLIKAVCYGCSKMMGALEIAEREGLPLNGDMSNHVP
                     LAPYIDRGATVITF"
     gene            complement(2869..3183)
                     /locus_tag="PLD15_10810"
     CDS             complement(2869..3183)
                     /locus_tag="PLD15_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPB64444.1"
                     /translation="MTLKDAFEKIGNLNPETLEGIEGSFSFRVRGGDPEEFTVVIASG
                     RAEILPNGGNSDCLVETDIETFRDVFEGSISPLSAFMSGRIKLSGDLSHAMKLSKLLG
                     GV"
BASE COUNT          715 a          934 c          830 g          839 t
ORIGIN      
        1 cttaggctag tcaacctgag ctactagctc ccggctttag ccgggggtcg ttgacatctt
       61 tccccatcct tttgacagtt ccttcaactc ttcgaagtag tctttatcgg tcattatggc
      121 atgaaggggc gtgaccgaaa cgtagttctc ttttaccgcg cagaaatcgc tgtccagtga
      181 gcattcgtcc tcgacaactt ctccgaatat ccagtagtag ttattgccgt acggatcctt
      241 ccgcttctcg aagtagtctt tgtacctcct gcgactctgt cgggttatcc tcaaaccctt
      301 caggtcttcc ctcgccaccg atggcacgtt tatgttcagg gcggtgaagg gaggcatctc
      361 tttcacgtcg aacgtttcga gaaattcgag cataaaatcg gcggccgtgg aatagatggg
      421 gttgctccaa tcgctgctcg atacggcgat cgagggcgtg tccataacgg ctccctccaa
      481 agcgcccgat accgttcccg aataaagaac gtctataccc atgttctgtc ccttgttaat
      541 tccgctgata accagatcgc gcttgatttc cgaaatcgcc aggagaccga gcttgacaca
      601 atcggccggt gtcccgttga tcgcgtagcc tatcattctg ttgccgactt tcacttcctt
      661 ggcccagaga ggtgtcctta tggttatggc gtgcccggtg gcgcttctct caacgtcggg
      721 agcgaccacc agaacgttgt gcctttccga aagcctgtcg gccagttcga ttatccccgg
      781 agacattatt ccatcgtcgt tggtaatcaa tatattcaat acactgcctc ctctcgcatc
      841 ttcatactgt tcttgtagta ttattttaca atatcgaacc ggacttttct ggaaagatcg
      901 ggtcccgttt cacttatgga gaggaaattg aagatagcgc agttgttcga gagacacccc
      961 gaactcgaag agatctacgg gctggcgcgg gtcgccttcg aagcctcccg cgatccggcc
     1021 cacgatcttt accacgtcgt gagggtcaca gaaaacgcgg tcgagatagc gctcggggag
     1081 gatgaatcgc tggagaccgc cgccctcgct gccctcctcc acgatataaa gaggccgtcc
     1141 gaggatatag acggtctcga tcatgccgcc gagggaggca agtactcgct caaactgctt
     1201 ctgagcatgg gatacactta cgacatcgcc tcggaagtag ccctgtctat aagagaccac
     1261 cgtttttcat ccgggagaga gcccgggagc gctaccggaa agattctcca ggatgcagac
     1321 agactcgacg ccatcggggc gatcgcgata gcgcgtgtct tttcttattc gggaaaggtc
     1381 ggcaggccgc tccattcacc ggacctgtcg ccgcgcgacc gttacgaagg tttctccgcg
     1441 agcgccataa accatttcta cgagaaaatc ctgaagataa cccccgaaag tttctggacc
     1501 ggtacgggca gggagctggc acgggagagg tacgaattca ccgtcgcctt cgtggagagg
     1561 tttctcgagg aatggtaggc tttcagtacc tgacttcgaa gtcggtgaaa tgctgctcgg
     1621 gtagttcttc gaccaggcat ttcgaaatat gcgcccgtac gggaccgcga tcgaccctct
     1681 gaacgaactc gtctatggaa gcctcttcgc cggcgccaac tatttcgacg ctaccgtcgt
     1741 actcgttcct cacatatccg gttatgccca gcgcccgtgc agtgtgcagg gtgtagtatc
     1801 gaaagcccac accctggacc ctgccgtaaa cccttaccct gacggttctc ataaacccag
     1861 ctcttcaccc acgagtatca ccgtgtatct tcccttgcgg cggctggaat cgcttacggt
     1921 cacgtaagtt ccgcagatcc tttcgttgct tgtacagtcc atgcagaagc ctgtggcggt
     1981 gcacggagtg tggagattca atcttttcgc gttcatcggc gctatgaacc ttatcctttc
     2041 gagcgccgca tcaatgtcgc cgacgacttt gttcatgccg acaacgagaa tgaccctttt
     2101 cggaccgaac aaaacggccg ccgcacggtt gcagttaccg tccagcagga gtatctgtcc
     2161 gtcgagtgtg atggcgttcg cgctacagag gtagaaatcc gcccagaaag cctttcgcaa
     2221 gatctcctct ctctcttctc cgtccggtac tgcttcgcgg tccagatagt tgtaacgccc
     2281 cgatttgaga agctctctcg cccccgtctc ttgaagagtg accgatccgc cggaggccac
     2341 cgtagaaccc gcggggatca gctcctcgag tctgggaacg acctgctctc tcgaggtaag
     2401 atagacggcc tctatagatc tttttgcgaa gttttcgatc aacttcccgg ccagtttgtc
     2461 gtatttccag ttcaggagac tgctacgcat atttcacccc tttcaggaat aacccggtta
     2521 gaaagttatg actgttgcgc ccctgtcgat gtagggagcc agcgggacgt ggttgctcat
     2581 atcgccgttc aacggtaatc cttccctttc tgctatttcc agagcgccca tcatcttcga
     2641 gcagccgtag cacacggcct tgatgagccc tttgcttttc gccttgctgt agagagggaa
     2701 gagaggatcg ctctccagag ttatctcttt cagcagagat ggcgactgcc cctcgagaac
     2761 caccgaaacg tcgaagcctt tttcgtgcat atccagcgcg tttagaaggg cgtggagaaa
     2821 acagggcttt tttccttcga atgcaagtat cagaacttct ttagccattc atacacctcc
     2881 gagtaatttg ctcagtttca tagcgtggga aagatccccc gatagcttta tcctgccgct
     2941 catgaaagcc gaaagcggac tgatcgaacc ttcaaataca tctctgaaag tttcgatgtc
     3001 ggtttctaca agacaatcgg agtttccgcc gttcgggagt atctccgccc tgcccgaggc
     3061 tatgacgacc gtgaactctt ccgggtctcc gcccctgaca cggaaggaaa aactcccctc
     3121 gataccttcc agagtttcgg gattcaaatt cccgattttt tcgaacgcat ctttcagagt
     3181 cataaactcc tcctcgtttt tgctcttaga aaaagtctat catcttgtcg tagaattgag
     3241 agtgatatct atcgagattg ggggatgaaa aatggggaga agcttttacc gctacgcgga
     3301 ttcagttttg agagtcgc
//
LOCUS       DAOOLY010000268         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_35232_length_3295_cov_6.286728, whole genome shotgun sequence.
ACCESSION   DAOOLY010000268 DAOOLY010000000
VERSION     DAOOLY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35232_length_3295_cov_6.286728"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="ggt"
                     /locus_tag="PLD15_10815"
     CDS             complement(<1..1196)
                     /gene="ggt"
                     /locus_tag="PLD15_10815"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPB64445.1"
                     /translation="MRKAILVSLMALMIVSMFAVNPLNLYGRDAEGRNGVVAAAKPEA
                     SEVGVKILEMGGNAVDAAIATAFALGVLEPNASGLGGGGFMIIQLAHMDEAVVIDFRE
                     TAPSAAGPTFFNLDERNRVINNETIIGGKSSGVPGEVAGLLYALENFGTMSRAQVIQP
                     AIEWAEKGIPVTVNLFSIINDNYEKIMMMENGAELYLKDGGIPYEIGETIVLKDLADT
                     LRIIVEKGKDGFYKGEIAEKIVAEVQKRGGVITLEDLANYDLQIRKPVVGTYRDYTIL
                     SVPPASSGGTHIIELLNIMENFDLATLGDNTPETLHLWSEAMKLIFADRSKYMADTAF
                     VDVPLTGLTSKEYARTLAAKIDPNKPMESVAAGDPWQYESGSTTHLSVMDKEGNMVAI
                     TKSINY"
     gene            1380..2198
                     /gene="nadE"
                     /locus_tag="PLD15_10820"
     CDS             1380..2198
                     /gene="nadE"
                     /locus_tag="PLD15_10820"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPB64446.1"
                     /translation="MQFIEKSVRENNYKGAVIGISGGIDSAVVGKLAVEALGKERVFG
                     LLLPERDSSARTMRDSRLVVDFLGIEHKIKRISGALRAIGAYHLQPPALLIPSKIKEN
                     FVKSKWKELSDDSFLDDLMNKGNEEFLKGLAYYRSKHRIRLVALYLEAEKRNYAVLGT
                     TNKTEKLCGFYVKWGDDSSDIEPIIHLYKTQVYTLARDLKIPRSIIDKPPTPDLVPGI
                     TDEFALGMKYSELDAILEKIERDEETTPPDSKVGRVRKILEAAKFRDTRNLSIQ"
     gene            complement(2188..3261)
                     /locus_tag="PLD15_10825"
     CDS             complement(2188..3261)
                     /locus_tag="PLD15_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64447.1"
                     /translation="MKALGYSILSITDAGSVFIEESKHDGVTFSFPSDPIFAKYRDQL
                     MVVIEVKMDSSKFSLTKSKLDLTSVRLHGVKIVDNKPFDDSVALWAFESRTLGKTMRV
                     MSGSEGSRVPAKREADYIVPAIVNTIELASKEENVGRFIFPLPGEWFVLSQISMGQDT
                     AKIRRENARLDLKSFSTEIIVAELKKHRVFTENLWNLYTLYKELDKFETKNSELTEPM
                     EEELTVSDLDLEETIPESVDLYLDGDEKTTVALHKEVLVQSSIEKVGTFSKKKAVVLR
                     IRNNSDLGTVKEVHFSFRLLDPVGKELYFAEHDLKDLNVCPGEAQKRDFTDFPEFTDQ
                     QLERVEVQLLKILWSNGVETFTE"
BASE COUNT          791 a          863 c          820 g          821 t
ORIGIN      
        1 aagtagttga tggatttggt aatggccacc atgttccctt ccttgtccat aacggaaagg
       61 tgagtggtgc tgccgctttc gtactgccat ggatcaccgg ccgcgaccga ttccatcggc
      121 ttgttgggat cgatcttagc tgctagagtc ctggcgtact ccttcgaagt cagtccggtc
      181 agcggtacat cgacgaaagc ggtgtctgcc atgtacttgg acctgtcggc aaagatcagt
      241 ttcatggctt ccgaccatag gtgcaacgtt tccggggtgt tgtcgcccag ggttgccaga
      301 tcgaagttct ccattatgtt cagcaactct attatgtgtg taccgccaga actggccggt
      361 ggcacggaga gaatcgtgta atccctgtag gtaccgacaa ccggcttcct gatctgcaga
      421 tcgtagttgg ccaggtcttc aagggttata actcccccgc gcttctgaac ctcggccacg
      481 attttctcgg cgatctcgcc cttgtagaaa ccgtccttac ctttttcgac gatgattctg
      541 agcgtgtcgg ccagatcctt gagaactatc gtctcgccga tttcgtaagg tatgccgcca
      601 tctttcagat ataactcggc cccattctcc atcatcatta tcttttcgta attgtcgttg
      661 atgatcgaga agagattcac ggtaacggga attccttttt cggcccattc gatggccggc
      721 tggataacct gggccctcga catggttccg aagttctcga gtgcgtagag cagtccggcg
      781 acttcgccgg gtacaccgct agacttgcca ccgataatcg tttcgttgtt gataacgcgg
      841 tttctctcgt cgagattgaa gaaggtcggt ccggccgccg atggagcggt ctctctgaag
      901 tcgatcacaa ctgcctcgtc catgtgggcc agctgtatga tcataaatcc accgccgccc
      961 agtcccgagg cgttgggttc gagaacaccc agcgcgaagg cggttgctat cgccgcatcc
     1021 acggcgttac ctcccatctc cagtatcttc acaccgactt ccgatgcttc gggcttggcc
     1081 gcagccacga ctccgtttct tccttcggca tcccttccgt aaaggttcaa tggattaact
     1141 gcaaacatag atacaatcat gagcgccatt aaggaaacca gaatagcttt tctcactttt
     1201 tcccctcctt gtttatagat tatgaaccgt gtttagagag tatagcatcc agaaatacca
     1261 aaaaagaatt aactgagagt gtctcggccg gctgtggcgg gaaccgtacc gggaaaaagt
     1321 tagtcaaaac aaataaccga tacaggtatc gggaggcgat cgaaataaaa gaggcaattg
     1381 tgcagttcat cgagaaatcg gttagagaga acaactacaa aggcgcggtt ataggcataa
     1441 gcggggggat agattcggcc gtagtcggta agctcgccgt tgaagcactt ggaaaggaaa
     1501 gagtcttcgg gcttctccta cccgagaggg attcttcggc ccggaccatg agagattcga
     1561 gactggtggt cgattttctg ggaatagagc acaagattaa gagaatatcg ggcgccttga
     1621 gggccatcgg agcctaccat ctccagccac cggccctgct gattccctcg aagatcaagg
     1681 agaacttcgt gaaaagcaag tggaaggagc tttcagatga ctcttttctc gacgatctta
     1741 tgaacaaggg taacgaggag ttcctgaagg gactggccta ctatagatcc aaacacagga
     1801 tacggctggt ggccctttat ctcgaagccg agaagagaaa ctacgccgtc ctgggaacga
     1861 cgaacaagac ggaaaaactg tgcggcttct acgtgaaatg gggcgacgac tcttccgata
     1921 tcgagcccat aatacacctc tacaaaactc aggtctacac tctggccaga gatttgaaga
     1981 taccgaggag tataatcgat aaacctccaa ccccagacct ggttcccggc atcacggacg
     2041 aattcgcact gggcatgaaa tacagcgagc ttgacgccat actggaaaag atagagcgcg
     2101 acgaagaaac aacccctccg gactcgaaag tcggaagggt gagaaaaatt ctggaagccg
     2161 cgaaattcag agacaccaga aatctatcta ttcagtgaaa gtctccacac cgttgctcca
     2221 aagtatcttc aaaagctgga cctccactcg ctcaagctgt tgatcggtga attccgggaa
     2281 gtccgtgaaa tctctcttct gcgcctcccc aggacacacg ttcaggtctt tcagatcgtg
     2341 ttcggcgaag tacagttcct tccctacggg gtcgagcaat ctgaaggaga agtgtacctc
     2401 cttaaccgta ccgagatcgg aattattcct gatcctcaga acgacggctt ttttcttgct
     2461 gaaagtgccg actttctcga tactcgactg gacgagaacc tctttgtgga gagctaccgt
     2521 agttttttca tcaccgtcca ggtaaagatc gaccgattcg ggaatcgttt cttcgagatc
     2581 gaggtcggag accgtcagtt cctcctccat cggttcggtc aactcgctgt tcttggtttc
     2641 gaatttgtcc agttccttgt agagagtgta gagattccag aggttttccg tgaagaccct
     2701 gtgtttcttc aactccgcga cgattatctc cgtgctgaag gattttagat ccagccgcgc
     2761 attctcgcgc cgtattttgg cggtgtcctg ccccatagat atctgtgaaa gcacgaacca
     2821 ctcacccggt agcgggaaga tgaatctgcc aacgttttcc tctttcgaag ccagttcaat
     2881 agtatttacg atcgccggaa cgatgtaatc ggcttccctc ttggccggaa ctctggatcc
     2941 ctcgctcccg gacataaccc tcatcgtctt tccgagggtc ctcgactcga atgcccacag
     3001 cgcaaccgag tcatcgaaag gcttgttgtc cactatcttc accccgtgga gcctcaccga
     3061 tgtgagatcc agtttgctct tggtgagcga gaatttcgag gaatccatct tcacctcgat
     3121 aaccaccatc agttgatctc tgtatttggc aaagatcgga tcggaaggga aagagaaggt
     3181 aacgccgtcg tgctttgact cctcgataaa aactgagccg gcatcggtta tagagagtat
     3241 agagtaaccc aacgctttca tgtttaattc cccctttctt gaatctcctg ctaat
//
LOCUS       DAOOLY010000269         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_35945_length_3248_cov_3.968055, whole genome shotgun sequence.
ACCESSION   DAOOLY010000269 DAOOLY010000000
VERSION     DAOOLY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35945_length_3248_cov_3.968055"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLD15_10830"
     CDS             complement(<1..726)
                     /locus_tag="PLD15_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPB64448.1"
                     /translation="MKKIVLRLIIGVIIGIISWGVVSLILSEGLGESFSSITEIENVK
                     VLMEIDRDGLLTVTETIDYRFKKPYRGIYRELPADRAGSLYGEIEVTATGKEIKYIER
                     MGGASSRSIRLWFVPYNSSSVEPSGGQDRVTVTYRYTVRRAVEIGTNVAQIFRKVWGE
                     DTASWVKNVNATIIFPEGMQITNFYTHPAVVVTRSGNVYELEMKDVPPKSYVEFRAVV
                     PLQATSTMKQVNLATGGVFDSKFL"
     gene            complement(868..2733)
                     /locus_tag="PLD15_10835"
     CDS             complement(868..2733)
                     /locus_tag="PLD15_10835"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPB64449.1"
                     /translation="MRKARLLLFSIFFFIFFFTSCPLKNTFTLTMQKEGNGTTDPAIG
                     THQFGKNVNATVRAYPDSGWEFDSWEGGVATADATETTVLMDRDRTVKAKFVEINHIE
                     KAIEKLDPSVPLSFYHAEELLPLVEGNLDKFLQLLSQGSYLERWAAAYALQRSPLDSG
                     TLEELEEYLSDPDSTIKVLIAVAFQLNGDEKGKAVLEAMLADDNPMSFSVPPQRLSDF
                     SLVILNTYYPLEYPLAGYEEQTTSVTGGPCDYTVTVTAVFQGTGATDTLVETWETQAE
                     EIWNGPTGSREWGNGCCTVTFDFDFDVLEEGEDPPEGVHVIEVKKVSSAHTSWVATPL
                     PTPGSTETTTGEWDNLDSGPVVAHEIGHLMGLDDEYHYDEDGNYVNDNPQPEGSPPSI
                     MAQTWNGAQPLTQHMDAIMAAADIQCECDYSMTITPEYDVNISPGVHTVEVTVVRADG
                     TPAKGTTVTFAVIGNPNVEDTEVKTGEDGKASFTYSREFCYTNEDEIEAWAKCNAHAM
                     ALKQWISLSWIFPDFISPYNDQKDFPYSTPLTILFEVEPENAREAENLEFVVKLFQGE
                     KELFTWKGKERAVETGLLLEPGTEYRIEITPISGPPETEGNTAVSSFTTADFTTD"
     gene            2908..>3248
                     /locus_tag="PLD15_10840"
     CDS             2908..>3248
                     /locus_tag="PLD15_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPB64450.1"
                     /translation="MFRKLRRDDRKLSLEEGRDILERGIFGVLSTLGEDGYPYGTPLN
                     YVFDDGRIYFHSACEGHKITNIMFSSKVSFCVVDFVEILPSSFSTNYKSVVAYGIASL
                     VAEASEKLYAL"
BASE COUNT          764 a          824 c          755 g          905 t
ORIGIN      
        1 caggaatttg ctatcgaaaa caccgcccgt ggccagattt acctgtttca tagtcgatgt
       61 tgcctgtagt ggtactacgg ctctgaattc gacataggat tttgggggga catctttcat
      121 ctccagctcg tagacattcc ccgatcttgt cacgactacg gccggatgag tgtagaaatt
      181 agtgatttgc ataccttccg ggaaaatgat cgtcgcattt acatttttga cccagcttgc
      241 cgtatcctca ccccagacct ttctgaagat ctgggcgaca tttgtaccga tctctacggc
      301 ccttctaacc gtgtagcgat aagtgacagt cacacgatcc tgcccaccgg agggttctac
      361 actggaggag ttataaggca cgaaccacaa tctgatcgac ctggaactcg cgcctcccat
      421 acgttcgatg tattttatct ctttaccggt agcggtcact tcgatctcgc cgtaaaggct
      481 acccgcacga tcggccggaa gttctctgta gattcccctg taaggttttt tgaaacggta
      541 gtcgatcgtt tcggttaccg tcagcaggcc gtctctgtct atctccatga gtaccttcac
      601 attctcgatt tcagtgatcg aggaaaagct ttcgccgagg ccttcactga gaatcaagga
      661 aacgactccc cacgagatta tcccaattat tacaccgata atcaaacgaa ggactatctt
      721 cttcataata atctcacctc cagttgaatt ttacatcaca ctggcggttt gcagggattc
      781 tctcaaaatt tttcgtgaga tattctgtct ctgtttcagc tgaacgttaa ttttgcccgt
      841 tcacccaact tcacaagact tcttcactca atcggtcgtg aaatcagcgg tagtgaaaga
      901 gctgaccgcc gtgttgcctt cagtctcggg aggaccagag ataggtgtaa tctctattct
      961 gtattcagtt cccggttcta acaaaaggcc cgtctccacc gccctctctt ttcccttcca
     1021 cgtgaagagt tctttttcac cttgaaagag tttgacgaca aattcgaggt tttcagcctc
     1081 cctggcgttt tccggctcta cttcaaagag aatcgtcaga ggagtcgaat agggaaagtc
     1141 cttctgatcg ttgtaaggcg agatgaaatc tgggaaaatc cagctcaaac ttatccattg
     1201 ttttagagcc attgcgtgtg cgttgcattt ggcccaggcc tcaatttcat cttcgttggt
     1261 gtagcagaat tcacgagaat aagtgaacga ggcctttcca tcttcacccg ttttgacttc
     1321 cgtgtcttcg acgttgggat tgcctatcac ggcgaaagtt actgtggttc ctttagccgg
     1381 agtcccgtcg gctctgacaa cggttacctc gacggtatga acgcccggag agatattgac
     1441 atcgtactca ggggtgatcg tcatagagta atcgcattcg cactggatat cggcggccgc
     1501 cattatcgca tccatgtgct gcgtcaacgg ttgagcacca ttccaggtct gcgccatgat
     1561 gctaggaggg ctaccctccg gctgggggtt gtcgttgacg taatttccat cttcgtcgta
     1621 atgatattca tcgtcaagtc ccatcaggtg tcctatttcg tgcgctacca ccggcccgct
     1681 gtctagattg tcccactccc cggtcgttgt ttcagttgaa cctggtgtcg gaaggggcgt
     1741 cgcaacccat gaagtgtgag cactgctgac ttttttcact tcgataacat ggactccttc
     1801 agggggatct tcaccctctt caagcacatc gaagtcgaag tcgaaggtaa ccgtgcaaca
     1861 accgtttccc cactcccttg aaccggtcgg gccgttccag atctcctcgg cctgagtctc
     1921 ccaggtctcc acgagagtat ctgtagcgcc cgtaccttga aagacagccg tgaccgttac
     1981 ggtatagtcg catggacctc ccgtcacgct ggtagtttgc tcttcgtatc cagccagcgg
     2041 atattcaagt ggatagtagg tgttcaagat tacaagagag aaatccgaca atctctgcgg
     2101 tggtaccgaa aagctcatag gattatcgtc ggcaagcatc gcttccagca cggctttccc
     2161 cttctcatct ccgttgagct ggaaagctac ggctataaga accttaatcg tggaatcagg
     2221 atcagaaaga tactcctcca gctcttcgag cgtaccgctg tccagcgggc ttctctgcaa
     2281 agcataagct gccgcccatc tttctagata gcttccctga gagagtagtt ggaggaactt
     2341 atcgagattg ccttccacca gaggcagaag ctcttctgca tgataaaaac ttagcggtac
     2401 cgaggggtcc agcttttcaa tagccttctc gatatggttt atttcgacga atttcgcttt
     2461 tacagttctg tctctatcca tgagaactgt ggtttccgtt gcatctgctg tggccacacc
     2521 gccctcccag ctatcgaatt cccagccgga atctgggtat gccctgaccg ttgcgtttac
     2581 gttcttaccg aactggtgag ttccgatagc cggatcggtc gttccgttac cctccttctg
     2641 catggtgagc gtgaaagtgt ttttcaatgg acagctggtg aaaaagaaga taaaaaagaa
     2701 tattgaaaac aacaacaaac gagctttcct cattcgtaag cacccccttc ttttgagtgc
     2761 gatcgaggac gacacaaatg cgagaggaat tcgaaacggc tgagtttgcc tgttcaactc
     2821 aagtatacta cttcagtcta taatgtaata tagtgttgga ctctcacgtg ccaatatgct
     2881 tagtttacac cgaccgggag gtatggtttg ttcagaaaac tcaggagaga cgatagaaag
     2941 ctttcgctag aagaaggtag agatatattg gaaaggggga tttttggagt tctttcaacc
     3001 ctgggagaag atggctatcc ctatggcact ccgcttaact acgtatttga tgacggccgc
     3061 atatattttc atagcgcctg cgaaggccac aagataacca acataatgtt cagcagtaag
     3121 gtttctttct gtgtagtgga ttttgttgaa atacttccat ccagtttttc aaccaactac
     3181 aaaagtgttg tggcttatgg aatcgcctcg ttggtggccg aagcttcaga gaaactgtac
     3241 gctttgaa
//
LOCUS       DAOOLY010000270         3194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_36759_length_3194_cov_8.755336, whole genome shotgun sequence.
ACCESSION   DAOOLY010000270 DAOOLY010000000
VERSION     DAOOLY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36759_length_3194_cov_8.755336"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLD15_10845"
     CDS             <1..728
                     /locus_tag="PLD15_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPB64451.1"
                     /translation="GVFVWTIEWSLLTVLISFVIGLFLAMMLNDSKLRFRKLYRSLLI
                     VPYAMPSFISLLVWRYGFYSSEFGFFNRMLKNLAGISIPWLDSVFWARFSVLLTNIWL
                     TFPYMMLVSLGALQAISPHLLEAAKIDGAGSWQKFRKVTFPLVIVTLAPLLIGSFATT
                     FNNFTVIWLLTEGHPVMGIGDVAGATDILISYAYKLAFSGREGSEMALSAAVSVVIFA
                     IVVTISIISFRRTRVLEDVANEI"
     gene            718..1584
                     /locus_tag="PLD15_10850"
     CDS             718..1584
                     /locus_tag="PLD15_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64452.1"
                     /translation="MRYKRTARYVIGRIMRHGAIWIAIVFAVFPALFIIGTSFNPANS
                     INSTGIFPAKPTLDNYTSLLFGANRVFTMWMWNTLKICFVTSSISVFLCALGAYAFSR
                     FSFRGRRAGLLMILLVQMFPQTLGMTAIFLIMAWFSKNLPLLGLNTHAGLIFVYLGGA
                     IGFNTWLMKGYFDSIPRSLEEAAFVDGATPFRTFLSIVLPLAKPILVVVFIIQFIATY
                     SEYMIASTLLKGDNMYTLAVGLRLFVGQHYDDRWGPFAAAGVLGSLVIVAIFYSLQGQ
                     IVSGLTGGAVKE"
     gene            1599..2969
                     /locus_tag="PLD15_10855"
     CDS             1599..2969
                     /locus_tag="PLD15_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPB64453.1"
                     /translation="MELKPPEWANDAIIYQIFPDSFFNGEPYNDPPDTLDWGAPPERN
                     RFYGGDLQGIIYKLDYLQKLGVNCIYLTPIFDAPSNHKYDTRNYFKVDETLGGDEALI
                     DLVEQLHARGMRILLDGVFNHCGVEHPFFVDATRRGKESRYWSWFHIDSIPVVESPEP
                     NYRCFAGYGKMPEFDLSNPQVRDYLLDVVRYWFEKADIDGWRLDTVEYLHPDFVRAVR
                     EKSKEMKPDAYVLGELLHLGTSWFKGGYLDGGMNYSLRKYLLSFFVFNNIDAKTFAEK
                     LYVLRRSYPDWANYAMYNMIDSHDRPRITTLCNGRKEILKLILLFLFTYPGVPAIYYG
                     DEIGIEGGSDPDCRRSMIWDEKLWDRELLEFYKRLIELRQTSPALKKGAFIPRIAENG
                     LFTFEREYGNERVVVCLNNSPVPREYKIGKPYDVLLWGSARIDSCTVSLDPYGFTVLR
                     KATERG"
BASE COUNT          741 a          769 c          822 g          862 t
ORIGIN      
        1 tcggggtctt cgtctggacc atcgaatggt cgcttttgac ggttcttatc agctttgtca
       61 tcggcctgtt tctggccatg atgctgaacg acagcaaatt aaggtttaga aaactctaca
      121 gatcccttct gatcgttccg tacgcgatgc catctttcat ctctctgctg gtctggcggt
      181 acggttttta tagcagcgag ttcggctttt tcaacaggat gctcaaaaac cttgccggta
      241 tatccatacc ctggctggac tcggtcttct gggcgagatt ctcggtgttg cttaccaaca
      301 tctggctcac atttccttac atgatgctgg tttcgttagg agctttacag gcgatttcgc
      361 cacatcttct tgaagcggcg aagatcgacg gtgctgggag ctggcagaag ttcaggaagg
      421 tgacctttcc actggtgatc gtgacgctgg ctccgcttct aataggttca ttcgcgacga
      481 ctttcaacaa tttcacggtg atctggctgc tcaccgaagg ccatccggtt atgggaatcg
      541 gagatgtcgc tggcgcgacc gatatcctta tctcttatgc ctacaaactg gccttctcag
      601 gaagagaggg aagcgaaatg gcgctctcgg ccgcggtatc tgtggtgatc ttcgcgatcg
      661 tggttactat atcgatcatt agctttagaa gaaccagagt gctcgaggat gttgccaatg
      721 agatataaaa ggaccgctag atatgttatc ggcaggataa tgcgacacgg cgcgatctgg
      781 attgcgatcg tgtttgcagt ctttccggcg cttttcataa taggcacctc tttcaatccg
      841 gccaattcga tcaactcgac gggtatattc ccggccaaac cgaccctcga caattacaca
      901 agtcttctct tcggcgccaa cagagtattc acgatgtgga tgtggaacac cttgaaaatc
      961 tgtttcgtga cctcgtctat aagcgtattt ctgtgcgcgc tgggggcgta cgccttttcc
     1021 cggttcagct ttcgcggaag gagagctgga ttgttgatga ttctcctggt tcaaatgttt
     1081 ccacaaacac ttgggatgac ggcgatcttc ctgattatgg cgtggttcag caagaacctt
     1141 ccgctactcg gtctcaacac tcacgcaggg ctaattttcg tctatctggg aggagcgata
     1201 ggcttcaaca cgtggctcat gaagggatat ttcgacagta ttccacgctc tctggaagag
     1261 gcggcctttg tcgacggagc cacacccttc agaacgtttc tgagtattgt gctgccactg
     1321 gccaagccca ttctcgttgt cgtcttcatt attcaattca tcgccactta ttccgaatac
     1381 atgatcgcga gtaccctgct caagggtgac aacatgtaca ccctggcagt gggattacgt
     1441 ctgttcgtcg gtcagcatta cgacgacagg tggggaccct tcgccgcggc cggagtcctc
     1501 ggctcgctgg ttatagtggc gatcttctat tcgttgcagg gtcaaatcgt ctccggcttg
     1561 acaggcggag cggtcaaaga gtaaaaagga ggatgtaaat ggagctcaaa ccgcctgaat
     1621 gggcaaacga tgccattatt tatcagatat ttcccgactc tttcttcaac ggtgaaccgt
     1681 acaacgatcc tccagatact cttgactggg gggccccgcc cgagaggaat cgcttttacg
     1741 gaggggacct gcaaggaatc atctataagc tggattattt acagaagctt ggagtcaact
     1801 gcatatacct gactcccatc ttcgatgcgc cttcgaacca caaatacgac acccgcaatt
     1861 acttcaaagt agacgaaacc ctgggcgggg atgaggccct catcgacttg gtcgagcagt
     1921 tgcatgcgag ggggatgagg atcttgctgg atggagtttt caaccattgc ggtgttgaac
     1981 acccgttctt cgttgatgcg acgaggaggg gtaaagagtc ccgctactgg agctggtttc
     2041 acattgactc gattccggtt gtcgagagcc ccgaaccgaa ctaccgttgt tttgccggct
     2101 acggaaagat gcccgagttc gatctttcca accctcaggt gagagattat ttacttgacg
     2161 tggtccgata ctggttcgag aaggccgata tcgacggctg gcggctcgac actgtggaat
     2221 atctgcatcc cgattttgta cgcgccgttc gagagaaatc caaagaaatg aaaccggacg
     2281 cttacgttct cggcgagctt cttcatcttg gaacttcctg gttcaaggga ggttatctgg
     2341 atggagggat gaactatagt ttgagaaagt atcttctctc ctttttcgtc ttcaacaaca
     2401 tcgacgccaa gacgtttgca gaaaagctat acgtcttgcg gcgtagctac cccgactggg
     2461 cgaactatgc gatgtacaac atgatagaca gccacgatcg ccccagaatc accacactct
     2521 gcaacgggag gaaggagata cttaaactca tcctgttgtt cttattcacc tacccgggtg
     2581 ttcctgcgat ctattacggc gacgagatag gcattgaagg cggtagcgat cctgactgtc
     2641 ggaggtcgat gatatgggat gagaagctct gggaccggga acttcttgag ttttacaaaa
     2701 ggctcataga gcttcgacaa accagtccgg ctctgaaaaa gggagctttc ataccccgta
     2761 tcgccgaaaa tgggcttttc acgttcgaga gagagtacgg gaatgaacgg gttgttgttt
     2821 gtctgaacaa ctcacccgtt ccaagagaat acaaaattgg aaagccttat gatgttctgc
     2881 tttgggggtc tgcgaggatc gattcatgta ccgtgtcgtt agatccgtat ggtttcacgg
     2941 tattgagaaa ggcgacggaa agaggctgat tcatttgagc tatactctga gacgccgttt
     3001 cgcctctgct ttgtgacacg acgggaatac aatatccttt gaggatcaac ctttggggac
     3061 aaatcttttt agtgactcga ttatttacat gacatctcag gagaagaagt cgtttataca
     3121 aggctactct tttcgggaca gactccatta tttccattcc tgtaatatag ttcttgattc
     3181 atttagaaca gatt
//
LOCUS       DAOOLY010000271         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_36795_length_3193_cov_3.776291, whole genome shotgun sequence.
ACCESSION   DAOOLY010000271 DAOOLY010000000
VERSION     DAOOLY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36795_length_3193_cov_3.776291"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..154
                     /locus_tag="PLD15_10860"
     CDS             8..154
                     /locus_tag="PLD15_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPB64454.1"
                     /translation="MYAEITLMFINGQFSPLPYTYPNYRDRDYIEELMKIREQYLQKR
                     KERL"
     gene            151..1452
                     /gene="rimO"
                     /locus_tag="PLD15_10865"
     CDS             151..1452
                     /gene="rimO"
                     /locus_tag="PLD15_10865"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPB64455.1"
                     /translation="MNLGVLTLGCPKNLADMDNFAGIMKSQGHTIVDEIDMADLIVID
                     TCGFIEEAKKESIDEIFKAISARKGKPGLKIIAVGCLVQRYFDEMKSDIPELDGLIGV
                     TSPLTLARLIERGELFYFQEPEGVYGYFNRRATGYSAYVKIGDGCNRNCAFCSIPLFK
                     GLSVSRSLESLKSEALSLVRKGVKEIVLVSQDNTQYGRDLDGDLDLGRLLRELNDLNE
                     EFWIRVMYLHPDHVDLPLIDTMLELPKVVDYFDMPVQSGSDRILKAMGRTRNSSELGS
                     IFNFIRDKAPHSVLRTTIMLGFPGETEETFRETMNFVKSVEFDRLGGFVYSSEEGTDS
                     FNKRRTTTTVRAKEMLELLLQEQDGISAARLSQYRGKVLKVLVEEAGQSYSIGRAFNS
                     APEIDGVVVLKGHVEEGLFTNVRITDTFEHDMEGVVLDELA"
     gene            1439..1981
                     /gene="pgsA"
                     /locus_tag="PLD15_10870"
     CDS             1439..1981
                     /gene="pgsA"
                     /locus_tag="PLD15_10870"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPB64456.1"
                     /translation="MNLPNLLTLSRIVLTVPALFFLTAGNGTVLYFLSAVVFVVAALT
                     DFFDGRIARKRGLVTDLGKFLDQLSDKILVTSIFLGFLTLSKISVWFLFVMIFRDTMV
                     SGIRMMASNRGRVIAADLFGKAKTVSQMVFIGILYLNVIWGLFGPPLIVVIELLVAAL
                     TLASGFNYLRKNLQIFKGGS"
     gene            1983..2555
                     /gene="thpR"
                     /locus_tag="PLD15_10875"
     CDS             1983..2555
                     /gene="thpR"
                     /locus_tag="PLD15_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPB64457.1"
                     /translation="MRSFIAIDTGQQVQTMVESVVEKLKRMGFRASWVPGGNSHVTIS
                     FLDNVEVQRLELLATMLSKRLRGFPAFTLETGKLGYFKHKELPKVVWIGIERNQTLSN
                     LYREVRSVLESMNFPVEDNFRPHLTVGRMKFSPPLWRKFLETIETEKIVFQVNEVSVF
                     ESILSREGAKYREVFTCSFEGGLIKHDFEG"
     gene            2539..>3193
                     /gene="recA"
                     /locus_tag="PLD15_10880"
     CDS             2539..>3193
                     /gene="recA"
                     /locus_tag="PLD15_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPB64458.1"
                     /translation="MISKDKKNALERAIKDIEKQFGKGSVMLMGEREDQNIEVISTGS
                     LALDIALTVGGYPRGRVVEIYGAESSGKTTIALHAIAEVQKRGGIAAFIDAEHALDIN
                     YAKNLGVDVDNLLVSQPDYGEQALDIVDGLIRSNAVDLIVVDSVAALVPRTEIEGAMG
                     ELQVGLQARLMSQALRKISGNVNRSKCIVVFINQTRMKIGVVYGNPETTTGGVALKFY
                     "
BASE COUNT          848 a          704 c          868 g          773 t
ORIGIN      
        1 tgacggaatg tacgccgaga ttaccctgat gttcataaac ggtcaatttt caccgctgcc
       61 ctacacttac ccaaattaca gggatcgcga ttacatagaa gaactcatga agatccgtga
      121 acagtacttg caaaagagaa aggaaaggtt atgaacctcg gggtactcac tctgggttgt
      181 cccaagaacc tggccgacat ggacaacttc gcgggaatta tgaagagcca gggccacacg
      241 atagtcgatg aaatcgacat ggccgatttg atagttatag acacttgcgg tttcatcgaa
      301 gaggcgaaga aagagagcat cgacgagata ttcaaggcca tttcggccag aaaagggaag
      361 cccgggctta aaatcatcgc cgtaggctgt ctggttcaga ggtatttcga cgagatgaag
      421 tcggatattc cagagctcga tggattgata ggtgtcacga gtccactgac tctggccaga
      481 ctcatagaga ggggtgaact cttttatttt caagagccgg aaggagtcta tggctacttc
      541 aaccgccggg caacaggtta cagcgcctat gtgaagatag gcgacggctg taatagaaac
      601 tgtgcctttt gttcgatacc tctcttcaag ggactctctg taagccgttc tctggagagc
      661 ctaaaaagcg aagcactttc ccttgtgagg aaaggtgtca aggaaattgt acttgtatct
      721 caggataata cccagtacgg cagggatctc gacggcgatc tggatctggg tcgtctgttg
      781 agggaactca acgatctgaa cgaagaattc tggataaggg ttatgtatct ccaccccgac
      841 cacgtcgatc ttcctctgat cgataccatg ctcgagttac ctaaggttgt ggattacttt
      901 gatatgccag tccagagcgg ctcggacaga atcttgaaag cgatgggcag gaccaggaat
      961 agttcggagc tcggttcgat cttcaatttt ataagagaca aggctcctca cagcgtattg
     1021 agaacaacta taatgctggg ttttcccgga gagacggaag agactttcag agaaaccatg
     1081 aacttcgtga aaagcgtcga gttcgacaga cttggaggat tcgtctattc gagtgaagag
     1141 ggaacagatt ctttcaacaa gagaagaacg accaccaccg tccgcgcgaa ggagatgctc
     1201 gaacttctcc tccaggaaca ggacggtata tcggctgcaa ggttgagtca gtacagaggt
     1261 aaggtgttga aggttctggt cgaagaggcg ggacagagct acagcattgg cagagccttc
     1321 aacagtgccc cggaaataga cggcgtggtc gtgcttaaag gtcacgtcga agagggactt
     1381 ttcactaacg ttagaatcac agatactttt gaacacgata tggaaggtgt tgttcttgat
     1441 gaacttgcct aatctattga ccttgagcag gatagtactc accgttccgg ccctcttttt
     1501 tttgacggcc gggaatggaa cggttctata ctttctgtcg gccgtggtct tcgtcgtcgc
     1561 ggcattgacg gacttcttcg acggaagaat agcccgaaag cgcggtctgg taaccgatct
     1621 gggaaagttt ctcgatcagc tttccgacaa gatactggtg acatcgatct tcctgggctt
     1681 tttgactctg tcgaagatta gcgtctggtt tctcttcgtg atgatcttca gagataccat
     1741 ggtatcggga atcaggatga tggcctccaa tcgggggaga gtgatcgcgg ccgatctttt
     1801 cggcaaggcc aagacggtat ctcaaatggt cttcattgga atcctttatt tgaatgtaat
     1861 atggggtctt tttggtccac cgctgatagt tgtcatagag ttgttggttg cggcactcac
     1921 actcgcgagc ggcttcaatt acctcagaaa gaatttgcag attttcaaag ggggtagttg
     1981 agttgcgctc attcatcgct atagacacag ggcagcaggt tcagacgatg gttgaatcgg
     2041 tggtcgaaaa actcaagaga atgggcttca gagcctcgtg ggtgccgggg ggcaactcgc
     2101 acgtcactat ctcattcctg gacaacgtgg aggttcaaag gctggagcta ctggctacga
     2161 tgctttccaa aaggcttcgg ggctttcccg ctttcactct cgagaccgga aagctcggtt
     2221 acttcaagca taaagaactg cctaaagttg tatggatcgg tatagaaagg aatcagacac
     2281 tttcaaacct ctacagagaa gtccgatctg tcctggaatc gatgaatttt cccgtcgagg
     2341 acaatttcag gcctcatctt acggtaggga ggatgaagtt cagcccgccc ctttggcgta
     2401 agtttctcga aaccatcgaa acagagaaga tagtatttca ggtcaacgaa gtgagcgtgt
     2461 tcgagtctat tctctccaga gagggagcga agtacaggga agtcttcacg tgcagttttg
     2521 aaggaggact gattaagcat gatttcgaag gataagaaga acgcacttga aagagcaata
     2581 aaggacatcg aaaagcagtt cggaaaaggt tcggttatgt tgatgggtga aagagaggat
     2641 cagaacatcg aagtcatctc caccggttcg ctggctctgg atatcgccct caccgtcggt
     2701 ggttacccga gggggagagt cgtcgaaatt tacggtgccg aatccagcgg caagacaacg
     2761 atcgcacttc atgcgattgc cgaggtacag aagcgcggcg gcatagcggc tttcatagat
     2821 gccgaacatg cgctcgatat caattacgca aagaatctgg gagttgatgt cgataatctt
     2881 ctggtttcac agcccgatta cggtgagcag gcactcgata tagtcgatgg actcatcaga
     2941 tccaacgccg tagatcttat agtggttgac tccgtggcgg ccctcgtacc gcgtaccgag
     3001 atagaaggag ccatgggcga actgcaggtc ggcctccagg ccagacttat gtcgcaggct
     3061 ctgagaaaga tctccggcaa tgtgaacaga tccaaatgca tagttgtctt catcaaccag
     3121 acaagaatga agatcggtgt cgtttacgga aatcctgaaa ccacaaccgg tggagtagca
     3181 ctgaagttct act
//
LOCUS       DAOOLY010000272         3180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_36983_length_3180_cov_6.059520, whole genome shotgun sequence.
ACCESSION   DAOOLY010000272 DAOOLY010000000
VERSION     DAOOLY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36983_length_3180_cov_6.059520"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1666
                     /locus_tag="PLD15_10885"
     CDS             371..1666
                     /locus_tag="PLD15_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64459.1"
                     /translation="MKKIFFVAFLIIFLVVTASARLVYWHTQEEESRQQVIAQIMESF
                     EIETGIEVEVVAIEENAMFSRLAAAAAAGTLPDVIEAGAETILGLGADGLLDTAIPTR
                     FIDNAGDFYTGATRFVITPAGNEYFAIPYHGWVQGIWYRKDWFEAAGLEAPTTWESIM
                     TAAKYFHKPEEGMYGIVLGKTNDAYAEQVFTIFSLSNGARIFDKDGNVTINSSQMKEV
                     LQYYKDLGAYSAPGHTYWKQARELYLASKTPMFFYSTYVMDDLALAEIQTTIISEFDP
                     DIVKNTEFAPFMSNSRSSSFGQVISLAVTKTSQNKLDVLKFLDFMFKPDNYIKYIHMA
                     PGGMLPTRSSVAESEAFINDPKGIYKSYGAEKIRSIIYGLENIEKFGYVEGKVFPEMG
                     KISGAFTIGNGIVMMFDNNATPDEVLTFWRDDIRKLIGR"
     gene            1724..2596
                     /locus_tag="PLD15_10890"
     CDS             1724..2596
                     /locus_tag="PLD15_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64460.1"
                     /translation="MQSVSTMLKNENRLGWRLVSPTVILLMILILYPVAYNIYISFFD
                     YGITRSTFVGIDNYLRVLKDGAFWKSFFITVGFTLMTVGGSLLLGLGVALMLNKEFKG
                     RSIVRTIVLLPYITPLISIVFSWQYIFDPSLGPFVEIFGRQLRWISPQLDLLNNSNNA
                     FIVASIFNIWRNFPFVYLMILSRLQSIPDEYYEAAEMDGASPWKKFTNITLPELYFVM
                     GAVALMRGIWNFYKFDEVYLISKKAGTLPIFIYERVIGTTSPEFGVAAAIATILMVIM
                     LGLITMYVKKVLKW"
     gene            2590..>3180
                     /locus_tag="PLD15_10895"
     CDS             2590..>3180
                     /locus_tag="PLD15_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64461.1"
                     /translation="MVKRASPFKKALFFTGIFLICFFILVPFYFMIHVSLKADYDPNK
                     MSFSDFTTRNYKEIFGLIQSSETEFFGDEITRANLEPVLKKIAELEEITSSREAYTEY
                     VHNVQLKEKEKELKDIVDLVIDFSGMKVEDREAFTERAMKNGTEEAIVLEENMKSIFG
                     ANDIKKFNDLRSEIEKALTDESISAGLERARSQLGAY"
BASE COUNT          862 a          760 c          766 g          792 t
ORIGIN      
        1 gcatttttct catcatttag cccccagcaa aatattgatt gttgcattcg attatatcac
       61 tagaaaaaca atgtcattcg tcgtttcggg tctttaagcc atggttgtgc gttgtgggta
      121 aaaacacggg aaggaacatc gagagttcgc ttcgctctcg ccctcttctc ggaaaacgtc
      181 tcctaacact attccgaagg tgatgctcga aatcctgtca cccattttcg accgatttat
      241 ttcgataatc ccctcagaag acctctcatc atctctcaac gctcctcttc atagctcata
      301 ggtttttgtt agcgatcgag ttcctgctat catagcaata cgaaaaaata ttcctttgga
      361 ggtgataaag gtgaaaaaaa ttttctttgt agcttttctt atcatcttcc tggtggttac
      421 ggccagcgcc agattagttt actggcacac ccaggaagaa gagagcaggc agcaggtaat
      481 tgcacagatc atggaatcct ttgagatcga gacaggcatc gaagtcgaag tcgtcgcgat
      541 tgaggagaac gcgatgttct caagactagc tgcggcagcg gcggccggga ctcttcccga
      601 tgtgatcgag gcgggcgccg agactatact cgggcttggt gccgacggtc ttttagacac
      661 ggccataccg accagattca tagacaatgc cggcgatttc tacacgggcg ctaccaggtt
      721 tgtcatcaca ccggcgggaa acgagtattt cgccattcca tatcatggat gggtacaggg
      781 tatctggtac agaaaagact ggttcgaggc tgcgggactt gaagctccga caacctggga
      841 atcgataatg acggcagcca aatacttcca caaacctgaa gagggaatgt acggcatcgt
      901 tcttggaaag acgaatgacg cttacgccga acaggttttc actatcttct cactttccaa
      961 cggagcgcgt atattcgaca aagacggcaa tgtaaccata aactcttcgc agatgaaaga
     1021 ggttctccag tactacaaag acctcggtgc ctattccgct ccggggcaca cctactggaa
     1081 gcaggcaaga gaactctatc tcgccagcaa aacaccgatg ttcttctatt ccacgtacgt
     1141 tatggacgat ctggcgctcg ccgagatcca gacgactata ataagcgagt tcgatccgga
     1201 tatagtcaag aacactgagt tcgctccctt catgtccaac agcaggtctt cctcgttcgg
     1261 tcaggtcatt tccctcgcag tgacgaagac ctcgcaaaac aagctcgatg tactcaaatt
     1321 cctcgatttc atgttcaaac cggacaacta tataaagtac atacacatgg cccctggcgg
     1381 catgcttccc accagatcat cggttgccga gagcgaagcc ttcataaacg atccgaaggg
     1441 aatctacaag agctacggtg cggaaaagat cagatcgatc atctacgggc tggagaacat
     1501 cgagaagttc ggatatgttg aaggcaaggt ctttccagaa atgggtaaga tatccggagc
     1561 cttcacgata ggcaatggaa tagtaatgat gttcgacaac aacgcaacac ccgacgaggt
     1621 cctcaccttc tggagagacg atattagaaa attaattgga aggtagtgtc ttccgggaag
     1681 gccccggttg agcggggcct tccttttttg gaggtcgttt ctcatgcaaa gcgtttccac
     1741 tatgttgaaa aatgaaaaca ggcttggctg gagactggta tcacccacgg taattcttct
     1801 catgatactg atcctctacc cggtcgccta caacatctat atatcctttt tcgattatgg
     1861 aataaccaga tcgactttcg tcgggatcga taactatttg cgagtcctga aagacggggc
     1921 cttctggaag tcattcttca taaccgtcgg ctttacgcta atgactgtcg gtggaagtct
     1981 tctgctcggt cttggagttg cgttgatgct gaacaaggaa ttcaagggca gatcgatcgt
     2041 gagaactata gttcttttac catacataac gccactgatt tcgatagtct tctcgtggca
     2101 atacattttc gacccgagtc tcggcccctt cgtcgagata ttcggcaggc agctcagatg
     2161 gatatcgcca cagttagatc tgctgaacaa ctcgaacaac gcctttatcg tggcttcaat
     2221 tttcaatatc tggcgcaact ttcccttcgt ctatctcatg atcctttcaa gattacagtc
     2281 gattcccgac gaatactacg aagccgccga aatggatggg gcatcgccct ggaaaaaatt
     2341 cactaatatc acattacccg agctctactt cgtcatgggt gcagtggcct tgatgagggg
     2401 gatctggaat ttctacaagt tcgacgaggt gtatctaata agcaagaagg ccggaacgct
     2461 gcctatcttc atatacgaga gggtcatagg gaccacatcg cccgagttcg gtgtggctgc
     2521 cgccatcgcg accattctga tggttataat gcttggcctg atcactatgt acgtgaagaa
     2581 ggtgttgaaa tggtgaagag agcctcgcca ttcaagaagg cgctgttttt cacgggtata
     2641 tttttgatat gtttcttcat tctggtgccc ttctatttta tgatacacgt atctctgaaa
     2701 gccgattacg acccaaacaa gatgagcttt tccgatttta ccaccagaaa ttacaaggag
     2761 atcttcggac tcattcagag cagcgagacg gagttcttcg gcgacgaaat aacgcgggcc
     2821 aacctcgagc ccgtgctgaa gaagatcgcc gaactggagg agataacttc ctctagagaa
     2881 gcttacacag aatacgtaca caacgtgcag ctcaaagaga aggagaaaga actgaaggat
     2941 atagtcgatc tggttataga cttcagcgga atgaaggtcg aagaccgtga agctttcacc
     3001 gagagagcca tgaaaaacgg taccgaagag gcgatcgtac ttgaagaaaa catgaaatcg
     3061 atcttcggtg ccaacgacat aaagaaattc aacgatctca ggagcgagat cgagaaggcc
     3121 ctgacagacg agtcgatatc ggcgggactg gaaagggcca ggtcacaact cggggcttat
//
LOCUS       DAOOLY010000273         3169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_37159_length_3169_cov_5.430957, whole genome shotgun sequence.
ACCESSION   DAOOLY010000273 DAOOLY010000000
VERSION     DAOOLY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37159_length_3169_cov_5.430957"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..905
                     /locus_tag="PLD15_10900"
     CDS             183..905
                     /locus_tag="PLD15_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64462.1"
                     /translation="MKKILIISVLALVIGVIVVAAPVKRSLGDNEIVRLQHQLQIANF
                     LNALELSDVQLKAIYDLAVATRENLENLQERIKGTLEESLEKAIAGEKVTPLQRADIV
                     KRVQEIVKEYVAGLKTIITVDQSEKLARYLRAKITEKLPAANERVEALKERLMENLPQ
                     VEERLKERLKSLPPQALERLKNSNALERVQDTREKVVTRELESDSKVTTRSVRVSVDI
                     QRLSLVLLSDAALEVMEKMLEQ"
     gene            complement(947..1585)
                     /locus_tag="PLD15_10905"
     CDS             complement(947..1585)
                     /locus_tag="PLD15_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPB64463.1"
                     /translation="MIKNLIWDFDGSLFDTYPLMVEIFQSALVEFGHTASREQILFLM
                     KDTLGKAVEHYLELGVGEGFYRLFLLREEAADPSLQPPFPYARRICLKVRDRGGKNLI
                     ITHRSRSTVMKLLYHHDMASLFSEIITKESGYRRKPDPEAFVAAIASHRLIAEETLSI
                     GDRDLDILAARAAGTRTCFFSQNGFSPSIPVDLIIHGLEELESFMDGDGSSK"
     gene            complement(1578..2240)
                     /locus_tag="PLD15_10910"
     CDS             complement(1578..2240)
                     /locus_tag="PLD15_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPB64464.1"
                     /translation="MFEYVIWDFGGTLFDTYPAATRVFKEVLSKFGVTAKEEEILEKL
                     KESTFKAATYFSNKYRLKEGLIEKFYTIENHLEPDKQPPFKSAREVCLQINRNGGNNF
                     LFSHRSNYSMTKLLSYYKMVDLFSEIVSSDNGFARKPDPQAIMYIIDKYDLDHEKVIT
                     IGDREIDIESGRRAGIATCLFNPGDFITSTRADYVIDSLEQLPAILQVCNRFKGCGDT
                     ND"
     gene            complement(2345..2848)
                     /locus_tag="PLD15_10915"
     CDS             complement(2345..2848)
                     /locus_tag="PLD15_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPB64465.1"
                     /translation="MKEVTLDIMIEIPKGSRNKYEFDKKLKMVRFDRTLFSAVHYPMD
                     YGFILNTLAEDEDPLDAMVLLWEPTFPGCLIEAKPIGAFKMWDEKGPDEKVLCVPIHD
                     PVWNYIETLEDVPPHLLKEIEHFFSVYKELEKKKTGIEGWVGKEETLRIIEEAKVRYI
                     ESGAKFF"
     gene            complement(2917..>3169)
                     /locus_tag="PLD15_10920"
     CDS             complement(2917..>3169)
                     /locus_tag="PLD15_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64466.1"
                     /translation="EFSSTVSYDGGAFTLPDYAPAPQYMLTSEPFPGLEDFLADKTVV
                     FTGYVEYDNDPDRRIYFQRALPAGIGFISDGWSIYFSAF"
BASE COUNT          830 a          782 c          747 g          810 t
ORIGIN      
        1 agagtaaccc aacgctttca tgtttaattc cccctttctt gaatctcctg ctaataattg
       61 cattataccc catgaggatg gcctgaaata agactattca acaacgataa atatttcacg
      121 ttcatttcac attgggcttc atataattat ctcagctaaa attaaattca gaggtgttac
      181 ccatgaaaaa gatactcata atttccgttc tggctcttgt aattggagtc atagttgttg
      241 ccgcacccgt gaaaagaagt cttggcgaca acgagatcgt cagactccag catcagttac
      301 agatagcgaa ctttttgaac gcgctcgaac tctctgacgt tcagttgaaa gcaatctacg
      361 atctggccgt tgcaacacgc gaaaaccttg aaaacctcca ggaaaggatc aagggaactc
      421 ttgaagaaag cctggaaaag gccatcgccg gcgagaaagt tacaccgctt caaagggccg
      481 atatcgtaaa gagagttcag gagattgtca aggaatacgt cgccggtctg aaaacgataa
      541 ttaccgtcga tcagagcgag aaactggcaa ggtatttaag ggcgaaaatc acggaaaagc
      601 tccccgcggc aaacgaaaga gtcgaggctc tcaaagagag attgatggaa aaccttccac
      661 aggtcgaaga aagacttaag gagagattga aatcccttcc tcctcaagct ctggagagat
      721 tgaaaaattc gaacgcccta gagagagtgc aagatactag agaaaaggtc gtcacaaggg
      781 agctcgagag cgattcgaaa gtcaccacca gaagtgttcg ggtgtcggtc gatatccaaa
      841 gactctccct ggtactactg tcggacgccg ctctagaagt tatggaaaag atgctggagc
      901 agtgagtagt tttgttcgga cgggacacgc ttttcgtgtc ccgttttcat tttgaggatc
      961 catcgccgtc cataaagctt tccagttctt ccaaaccatg gataatcagg tcaaccggaa
     1021 tagaaggaga aaagccgttc tgcgaaaaga aacaggtcct ggttccggca gccctggcag
     1081 ccaggatatc gagatcacga tcgcctatag agagcgtctc ttcagcgatt aacctgtggg
     1141 aggcgatggc cgcaacgaag gcttcgggat cgggtttgcg cctgtagccg ctttctttgg
     1201 ttattatctc cgagaagaga gaagccatat cgtgatggta gaggagcttc atcaccgtgc
     1261 tcctggacct gtgcgttatt atgaggttct ttcctcccct atccctgact ttcagacaga
     1321 ttcggcgggc ataaggaaaa ggcggttgca gggagggatc ggccgcttct tcacgcagca
     1381 ggaaaagcct gtaaaaacct tcgccaacgc ccagctccag ataatgctcg acggcctttc
     1441 caagcgtgtc cttcatcagg aaaagtatct gttcccggct ggcggtgtgt ccgaactcta
     1501 caagtgccga ctggaatatc tctaccatca gcggataggt gtcgaagagc gatccgtcga
     1561 aatcccagat gaggttttta atcatttgta tctccacacc ctttgaaccg gttgcaaacc
     1621 tggagaatcg ccggcagttg ttcaagcgaa tcgattacat agtctgctct ggtagaagtt
     1681 atgaaatcac ccgggttgaa aaggcaggtg gcgataccgg cacgccttcc cgattctata
     1741 tctatctcgc ggtcgcctat cgttatcacc ttttcgtggt cgagatcgta cttgtcgatt
     1801 atatacatga tagcctgggg atcgggcttt ctggcaaatc cattgtcgct ggagactatt
     1861 tccgagaaga gatcgaccat cttgtagtag ctcagcagtt tggtcataga gtagttactt
     1921 cgatggctaa agagaaaatt gttcccgccg ttcctgttta tctggagaca aacctctctg
     1981 gccgatttga aaggcggctg tttatcgggc tccaaatgat tttcgatcgt atagaatttc
     2041 tctatgagac cttccttcag gcgatacttg ttcgagaaat aagtggcagc cttgaaggtc
     2101 gattctttga gcttttcgag tatctcttcc tctttcgcgg taactccgaa tttcgacagc
     2161 acttccttga aaaccctcgt tgcggccgga taggtatcga aaagcgttcc accaaaatcc
     2221 cagatcacat actcgaacat cgaaatctct ccttttcaac ttctacttct acaggtatca
     2281 taatacaaat cactaatcgg gcgtagaacg gctcctaacg ctctttacaa agacagctcc
     2341 tcgatcaaaa gaacttcgcc ccgctttcga tgtaccggac tttcgcctct tctattatcc
     2401 tgagcgtctc ttccttcccg acccagcctt ctattcccgt cttcttcttt tcgagttctt
     2461 tgtaaaccga gaaaaaatgc tcgatctcct tgagcaggtg gggaggaaca tcttccagag
     2521 tctctatata attccatacg ggatcgtgta tcggaacaca gaggaccttc tcgtcgggac
     2581 ccttctcgtc ccacatctta aaagctccta taggtttggc ttcgatgagg cagcccggga
     2641 aggtcggctc ccacagcaga accatcgcat cgagaggatc ttcgtcttcg gcaagcgtgt
     2701 tcaatatgaa accgtagtcc atcgggtaat gaacggccga aaagagcgtc ctatcgaagc
     2761 ggaccatctt cagctttttg tcgaactcgt acttgttacg actgcctttg ggtatttcga
     2821 tcattatatc cagtgttacc tctttcaccg tatcaccccc gccacaatta tgccagaaaa
     2881 aagccccctt gcgggggcta gatattgcat acggccttag aaggcactga agtaaatcga
     2941 ccagccgtct gaaatgaaac ctattccggc tggaagcgcc ctctggaaat atatcctcct
     3001 gtccggatcg ttgtcgtact ctacgtagcc ggtgaagacg acggttttat cggccagaaa
     3061 atcttcgagt cccgggaatg gttcgcttgt aagcatatac tggggtgccg gggcatagtc
     3121 cgggagcgta aaagctcctc cgtcatagga tacagtagaa gaaaactcc
//
LOCUS       DAOOLY010000274         3122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_37867_length_3122_cov_3.861102, whole genome shotgun sequence.
ACCESSION   DAOOLY010000274 DAOOLY010000000
VERSION     DAOOLY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37867_length_3122_cov_3.861102"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLD15_10925"
     CDS             complement(<1..509)
                     /locus_tag="PLD15_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPB64467.1"
                     /translation="MHITPEVRKAAMEIKTPFLIMDMDYVRNNYFDIVNHVRNVQVFY
                     AVKANSHPRIIETLRDLGSNFDVASRGEIEKLLSLGVTPERMSFGNTIKKVEDIAFAY
                     SVGIDYYAVDSEMEVEKIAAHAPGSKVYGRIATSGGDCDWPLSRKFGTDVNHVISILE
                     YADQLGLDA"
     gene            complement(636..1586)
                     /locus_tag="PLD15_10930"
     CDS             complement(636..1586)
                     /locus_tag="PLD15_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPB64468.1"
                     /translation="MRSIFRDFTYDYFSILAYPKEKWHDFWLIYREKHPRVLEEYMHK
                     NNLDDSSLSRELERLDRREIDRLSQYWQEAGPREKSRVLKELGTMSADLHLEREDFVI
                     HILGGLGKQEHLIVPTSKGNVVMIDLLHCWKNGSINDFLAVVNRALEDFLDYSRIHVR
                     NTMNPQQREERYTILHERLIKELDGLSFEHRMKRTVAFLDKYIEHYNWTGFYLSDGEN
                     YLVLGPYVGEPTEHTRIPFGRGICGQAAETRNVFLVPDVTKESNYLSCSPKTKSEIVL
                     PLIKEERVIGELDIDSHFQNSFFDSDREFLEKICQFLIGS"
     gene            complement(1596..2801)
                     /locus_tag="PLD15_10935"
     CDS             complement(1596..2801)
                     /locus_tag="PLD15_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPB64469.1"
                     /translation="MRRVQYLLFLLCVVLMALFFSLLVLLFRGSYSSIINLTTPGESF
                     LIVGIDTGGKANNSIGGRTDYIALFYLDDAGTFNIKSIPRDTVITHNGATRKINSLYN
                     SFGMNALISQVETLTKRKISGFVIVDFNTVNDLTDFTGPIRVDVLSPMHHDDYQQDLH
                     IHFETGVHYLEGEDLLKYLRFRSSDTGDLGRIERQKYIINQLVSQLIQAGPKKILELI
                     DYVMSKTEINVDKKTLLGVAYSFLRGSRSITFSQIDYYLDRDGQIIPREPAATTVQTP
                     VTPTVSSPTILVINNIPDYQTRLGDFVETVKSQWLKQASIKVEATGIVPEIPGIEKRE
                     TYLFINSRASEVRDAFSLAHLYHNPVVITTYNFANIEKYYALLDSLSKERFYPVTYDA
                     LVLLGVGGR"
     gene            complement(2801..>3122)
                     /locus_tag="PLD15_10940"
     CDS             complement(2801..>3122)
                     /locus_tag="PLD15_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64470.1"
                     /translation="ESLGIKASNAVQLIDGMAVRGFKLLDYWKLGGFSKVIKEFNPST
                     TITPKGMAVLLKRLSTSSRIAYQVETLTQYPLKIKVGVTGESVNRLYIKTDSIESVKK
                     ALAR"
BASE COUNT          726 a          760 c          741 g          895 t
ORIGIN      
        1 tacgcatcga gcccgagttg atcggcgtat tcgaggatag agataacatg gttgacatcc
       61 gttccaaact tccttgaaag cggccaatcg cagtcaccgc cgctggttgc tattcttcca
      121 taaaccttgc tcccgggggc gtgggcggcg atcttctcaa cttccatttc ggaatcgacc
      181 gcatagtaat cgatacccac cgaataagcg aaggcgatat cttccacttt ttttatcgta
      241 ttgccaaaac tcattctctc cggagttact ccaagggaaa ggagcttctc gatctctcca
      301 cgtgatgcta catcgaagtt gctgccaaga tcccgaaggg tttcgatgat cctgggatgt
      361 gaattcgcct taaccgcgta aaacacctgg acatttctta catggtttac tatgtcgaag
      421 taattgtttc taacgtagtc catatccatt atcaagaacg gtgtctttat ttccattgcg
      481 gcctttctta cttcaggcgt tatgtgcact acggcatccc tcccataaaa tgatcaagtt
      541 attgtagcac cattagtatg aaagaatgcc aaagataaaa tgtcagtttt agtttaaaaa
      601 agttacaata ctgaatggca atttgtcagc cactttcagc tgccaatcag gaactggcag
      661 attttttcca gaaactccct gtctgaatca aagaaactat tttgaaaatg actgtcaata
      721 tccagttcac ctataactct ctcttccttt atcaaaggga gtacaatttc cgatttcgtt
      781 ttcggactgc acgagaggta gttgctctcc ttcgtaacgt ccgggacgag aaagacgttt
      841 cgggtttctg ccgcctggcc gcaaatgcct cttccaaaag ggatcctggt gtgctctgtc
      901 ggttccccca cgtacgggcc gagcaccagg taattttcac cgtcactcag gtagaagcct
      961 gtccaattgt aatgttcgat gtatttatct agaaaagcca ccgttctttt cattctatgc
     1021 tcgaaagaca gaccgtcgag ttctttaatc agtctttcgt gaaggatggt gtaccgttcc
     1081 tctctctgct gggggttcat cgtgtttctg acgtgtatcc gcgagtagtc gagaaagtct
     1141 tccagagccc tattgacgac ggcgagaaag tcgtttatac ttccgttttt ccagcagtgc
     1201 aacaggtcga tcatcacgac attgcctttc gaagtcggca caatgagatg ttcctgcttt
     1261 ccaaggccac cgagtatgtg gatgacgaag tcctctcttt caaggtggag atcggccgac
     1321 atggttccga gttctttgag gacacgtgat ttttcacggg gaccggcttc ctgccagtac
     1381 tgtgaaagcc tgtctatctc tctcctgtca agtctttcca gctccctgct aagcgatgag
     1441 tcgtcgaggt tgtttttgtg catatactct tcgagaaccc ttggatgctt ttccctgtag
     1501 atgagccaga agtcatgcca tttctcttta gggtaagcca gtatgctgaa gtaatcgtag
     1561 gtgaaatctc tgaaaatgct tctcatagtc ctccttcatc ttcctccgac acccagcagc
     1621 accagggcgt cgtaagtaac cggatagaat ctctctttcg aaagcgaatc gagtagagcg
     1681 tagtactttt ctatattggc gaagttgtat gtagtaatca ccacggggtt gtggtataga
     1741 tgagccaaag agaaggcgtc cctaacttct gaggctcttg agtttatgaa taagtatgtt
     1801 tcacgcttct cgatacctgg tatctccggg actatgccgg tggcttcgac ctttatactg
     1861 gcctgtttca gccactggct cttcaccgtc tcgacgaaat cgcccagcct ggtttgataa
     1921 tcgggtatat tgtttatcac cagtatcgta ggcgagctaa cggtaggtgt gaccggtgtc
     1981 tggaccgttg tggccgctgg ctctctcggg attatctgtc cgtccctgtc caggtagtaa
     2041 tctatctggg agaaggtgat ggatctgctg cccctgagaa agctgtaagc aactcccaga
     2101 agcgtcttct tgtctacgtt gatctcggtc ttgctcatga cgtaatcgat caactccaga
     2161 atcttcttcg gtcccgcctg tatgagctgg gagaccagct gatttatgat gtacttctgt
     2221 ctttcgatcc tccccagatc acctgtatcg ctgcttctga agcggaggta tttcaaaaga
     2281 tcttcccctt caaggtaatg aacgccggtt tcgaagtgga tgtgcagatc ctgctggtag
     2341 tcgtcgtggt gcatggggga gagcacatct acccggatag ggccggtgaa atccgtcaga
     2401 tcgttgaccg tgttgaagtc tactatcacg aaaccggata tcttcctttt agtgagagtt
     2461 tccacctgtg agatcagtgc gttcataccg aaagagttgt agagagagtt tatcttcctt
     2521 gtcgcaccgt tgtgagttat gacggtatcg cgcggtatgc tcttgatgtt gaaagtaccg
     2581 gcatcgtcga gatagaagag agctatgtaa tcggtccggc caccgatgga gttgtttgcc
     2641 tttccaccgg tgtctatacc gactatgagg aaactctctc ccggggtcgt cagatttatg
     2701 atggaggaat aggaccctct gaagagtaag accagcaggg agaagaaaag agccattaga
     2761 accacacaga gcaagaagag aaggtactga actctcctca tcaccttgcc agtgcctttt
     2821 tcacactttc tatagaatcg gtctttatgt acagtctatt caccgactcg ccggtgacac
     2881 cgacttttat cttcagcgga tactgggtga gcgtctccac ctggtacgcg atcctcgaag
     2941 aggtgctcag ccttttcagg agaacggcca ttcccttcgg ggtgattgtc gtggaggggt
     3001 tgaattcttt tatcactttt gagaaacctc caagcttcca gtaatcgagc agcttgaaac
     3061 ccctcacagc cattccgtcg atcaactgaa cggcgttgga agctttgatc cctagagatt
     3121 cg
//
LOCUS       DAOOLY010000275         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_38716_length_3071_cov_2.889589, whole genome shotgun sequence.
ACCESSION   DAOOLY010000275 DAOOLY010000000
VERSION     DAOOLY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38716_length_3071_cov_2.889589"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLD15_10945"
     CDS             complement(<1..292)
                     /locus_tag="PLD15_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB64471.1"
                     /translation="MKYRARNGFSLVELLIVLAVVAMLISVITPLGINAIRKSRAIAI
                     SKDLKALSKTFVNKIYLDNEVPRELKELGRDVDSDLFGAGWKIYEDGQYDFFV"
     gene            complement(487..999)
                     /locus_tag="PLD15_10950"
     CDS             complement(487..999)
                     /locus_tag="PLD15_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPB64472.1"
                     /translation="MKRNAGLTLSEILVVLLISGIIMSIAFAVFSSGWRVYGATRETA
                     LLSRNLRNIVERISEELRWAREVEFGDSLQGNDWIVITLENGTIKRFQTSGQSRMNEQ
                     SLSDPSDIAVSNLSMTMRTVEDNRVMFDVSLTASSTKNGKISESVDTSIAAYNLRNIS
                     GSGSTIGYKK"
     gene            complement(1008..1337)
                     /locus_tag="PLD15_10955"
     CDS             complement(1008..1337)
                     /locus_tag="PLD15_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPB64473.1"
                     /translation="MKTNTNYKNRKSGFSLVEVLVSLIIVTVIISAVLPMLSSSMRRA
                     QQNKFKINELSGVQNELEEEMFVSSVGQSYIVNIALESESISLSGELITKNYADWKRV
                     DLFLRNR"
     gene            complement(1347..>3071)
                     /locus_tag="PLD15_10960"
     CDS             complement(1347..>3071)
                     /locus_tag="PLD15_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64474.1"
                     /translation="ENGNGSFELVEAWENGDNWESDLQTIRNTVLDVIQSSQSDIAFA
                     ILSNQSGSEFKIVSYGNRNTLVESVVLTLASSGGAYEFPIFDMAVFTDSYIELQGSAR
                     IEGKSGINSISPGSINIVGGEAEITGTIYVGVDGDTHVNPGVNPNNPNQSIYYPEAVV
                     TRTNIWNNTWLDTHPIENLTKTRLYTLPAFPDPPASIVFPTFPVFPEGLHQNGDWNIN
                     GSSTLTITQSGDYAKLSVAGSGRLIFDMGGEDLSIRANSLSVGGSGRIEVRGPGTLNL
                     YVEGNTELGGNGVSLINSARFNLYTNGNFSTSSGSNVRLSTVYAKGQTQLKGNLLDLE
                     NLYVDSNQSFSTSQNGIVRISSNSLVKTSSVSLSSGTIDFQNGPKQQFEVSGTMSLSG
                     NVIINGIGKGVIRAGTLNIGQGHINLAGGGKLEVYAITDFDMGAGGTLNNGGEVDAVR
                     VSYAGAKSLKLTGNIRFTGIVHIQRADVDVGGSAQINGLVISGGQTVNLTGDQLANVI
                     AVYAPDSTVNIGGSAQVRGAVVSDRLIATGNAKVVYETDIEETFPPELIGLVGGGQGE
                     IDAEIWSR"
BASE COUNT          773 a          800 c          626 g          872 t
ORIGIN      
        1 atacaaagaa atcgtattgc ccatcttcat atatcttcca gccagctccg aaaagatctg
       61 aatctacatc tcttccaagt tctttcaact ctcgaggcac ttcgttatcc aggtatatct
      121 tgtttacgaa ggttttcgaa agtgccttga ggtctttcga tattgcgata gcccttgatt
      181 ttcttatggc attgattccc aggggggtaa ttacagagat aagcatagca acaactgcga
      241 gcactatcaa aagctctact agagagaaac catttcttgc tctgtatttc ataactattc
      301 gctcctcctg aaaatcgttg tctttaaatt tggacaagca aaactgtgcc agatgtttcc
      361 atatgattct cataacagcc gatgtgcttt gtataggtct ctttgaagag aagcgtagcg
      421 ctcaagcaga tatagccact tttattaaaa gaagcttttg attatatttc caaaattgag
      481 aagcctttat tttttatagc cgattgtcga tcccgagcca gaaatgttgc gaaggttata
      541 agcagcgatc gatgtatcga cactctcgct tattttgccg tttttggtgc tcgaggctgt
      601 taaggataca tcgaacatca cacggttatc ctcgaccgtc ctcatcgtca tcgaaagatt
      661 cgaaacagct atatccgaag gatcgctcaa gctctgctcg ttcatcctgc tctgtccgga
      721 tgtttgaaac ctctttatcg taccgttttc cagcgtgatc actatccagt cgtttccctg
      781 cagagaatcg ccgaactcaa cttctcttgc ccatcgcagc tcttccgaaa tcctctctac
      841 tatattcctc aagtttctgc taaggagcgc cgtttcccgg gtagctccgt aaactctcca
      901 gcccgatgag aaaaccgcaa aggctatgga cattataatt ccagatatca ggagaacgac
      961 gagaatctcc gataaagtga gaccggcatt tctcttcatc tgaagcatca cctgtttctc
     1021 aaaaacagat ctacgcgttt ccagtctgca tagttctttg tgatcagctc tcccgaaagc
     1081 gatatgcttt cactttctaa agcgatgttc actatgtacg attgtcccac gcttgaaaca
     1141 aacatctctt cttccagctc gttttgaacc cctgacagtt cgttgatctt gaatttattc
     1201 tgctgtgccc ttctcatgct actggagagc atcggaagaa ctgccgagat aatcacggtg
     1261 actataataa gactcacaag aacctcgaca agagaaaaac cggactttct attcttataa
     1321 ttggtatttg tcttcatcct tatcacctac ctgctccaaa tttcggcatc tatttctccc
     1381 tggccaccgc cgacaaggcc tatcaactca gggggaaagg tctcttcgat atcggtttca
     1441 taaacaactt ttgcattccc ggtagcgatc aaacgatctg agacgacggc tcctctgact
     1501 tgtgcgctac cgccaatgtt gacagtcgag tcgggcgcat atacggctat cacattggcc
     1561 agttgatcgc cagtcaaatt caccgtctgg ccgccgctta taactagacc gtttatctgt
     1621 gccgagccgc cgacatctac atcggctctc tgtatatgaa cgattccagt aaaacggata
     1681 ttcccggtga gcttcaagct ttttgcgccc gcgtaactca ccctcaccgc atcaacctct
     1741 ccgccgttgt tgagcgttcc tcctgcaccc atatcgaaat ctgtgatagc gtaaacttcg
     1801 agctttcccc ctcccgccag attgatatgg ccctgcccta tgttcaacgt tccagccctt
     1861 ataaccccct tgcctatacc gttaatgatt acattacccg agagcgacat agtcccagaa
     1921 acttcgaact gttgcttcgg gccgttctga aaatcgatcg taccgctgga taaagagacc
     1981 gatgaggttt tcaccagact gttcgaagag atcctcacga tcccgttctg cgaggttgaa
     2041 aaggactgat ttgaatctac ataaagattc tccaggtcga gaagattacc tttcagctgc
     2101 gtctggccct ttgcgtaaac cgtggaaagc ctcacattgc ttccggagga tgtggagaag
     2161 ttgccgttcg tgtacagatt gaatctggcc gagttaatca aagaaactcc gttgcctccc
     2221 agttcggtat tgccttccac gtagagattc agagtccccg gcccccttac ctctatccgc
     2281 ccgcttccgc cgactgatag actgttagcc cttatcgaaa gatcttctcc tcccatatcg
     2341 aatatgagcc tgccactgcc ggcaacactc aattttgcat aatcgccgct ctgagtgata
     2401 gtaagagtag aactaccgtt tatattccag tcaccgttct gatgaagccc ctctggaaag
     2461 acaggaaatg tggggaatac tatcgaagcc ggcggatctg gaaaagccgg aagagtgtaa
     2521 agccttgttt tagtaagatt ctctattgga tgggtatcca accaagtatt gttccagata
     2581 ttagtcctcg taacgaccgc ctctggatag taaatcgatt gattcggatt gttcggattc
     2641 acgcccgggt taacatgagt atctccatcg acaccgacat aaatcgtgcc ggtgatttcg
     2701 gcttcgccac caactatatt tatcgatcct ggagagatcg agtttatccc gctttttcct
     2761 tctattctgg cgctgccttg aagctcgata taactgtctg tgaaaacggc catatcgaag
     2821 atggggaact cgtaagctcc acccgaagag gcaagggtaa gcacaacgct ctcgactagc
     2881 gtatttctgt tgccgtaaga tactatcttg aactctgaac ccgactgatt cgaaagaata
     2941 gcgaaagcga tatcgctctg agaagattga atgacatcca gcactgtgtt tctgatcgtt
     3001 tgaagatcgc tttcccagtt atccccattc tcccaggctt cgaccagttc aaaagaaccg
     3061 ttgccgtttt c
//
LOCUS       DAOOLY010000276         3070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_38724_length_3070_cov_4.817910, whole genome shotgun sequence.
ACCESSION   DAOOLY010000276 DAOOLY010000000
VERSION     DAOOLY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38724_length_3070_cov_4.817910"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLD15_10965"
     CDS             <1..274
                     /locus_tag="PLD15_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HPB64475.1"
                     /translation="TGTLSTGMALLREVDPTFETGTAEDMVFGSGLALFLGIPMLILL
                     NIPILGYREGKPIYYLYFLLGLAAYILVLYFFWFRKARIRKREKAN"
     gene            307..2151
                     /locus_tag="PLD15_10970"
     CDS             307..2151
                     /locus_tag="PLD15_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPB64476.1"
                     /translation="MRRLVIVVLFALLAIAVVASDLRLFYGNPHSHTSYSDGTGTPEE
                     AYEYGSKVPGLDFLAVTDHGYYFVQTLPDGRDKLTATIEAAQKATTGSFLAFAGFEWT
                     ATGTGHINVYGTQDWTDRVQSDLWQLYDWIIERKAVGQFNHPITMFGDNFKQFHYVPQ
                     ADLYVNLIEVGNGNWWQNETISQEMFQAYRLALLKGWHLGATLGQDNHKPNWGGANDS
                     RAVVYASGLTQEEILKAFSERRVYASEDRDILLSFSADSGFMGDILHDLEEINLKVSI
                     KDETGDDLEFVEIYSRTGLLAHFDVNDSLFEMELRLDIETGYEYFFVYAKARDGEEVV
                     SSPIWVQRSDPVHLYNPAVHPSNVKPGEEVTLSFQISNTSSQEASTTLELRNLHGVIL
                     QESYLLTAYESREVTLPHTTGNGDERIDFYLDSVPYSSVSLKLREATSLNVLLDRSHV
                     NYAMDRREKLSTTLQEFGHKYSTVERLLKTGELDNINVLIMPLPGAGGSFERLKMLMP
                     AQAGIIADFVRKGGTLIITGTGETVGQDIIDTYNKFLADLGLPVTFGGVQSGEIVSVE
                     GISFDGISTLEGEAGEYPAGDGTVIIYPGDPLTDNVIEMNEKLVWRLF"
     gene            complement(2427..>3070)
                     /locus_tag="PLD15_10975"
     CDS             complement(2427..>3070)
                     /locus_tag="PLD15_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64477.1"
                     /translation="SVEYYEAFERPKISYQKFQVSPAFAIDARGEFLTNDAVWNIGME
                     DYVLLATLNSILGWYLISRHCTEIQNGYQLIWTYLKNVTVVVPDSPTGKSIEAKTKEL
                     IDLKSDLSGRLSSCMELISTEYGLKKTGKNLQQFYRLGWNQFVWELEKQKARLTLGQK
                     EELNGWFRKKREELNELEEKIELLDREINLAIYELYRLNREDIDLVESSVKQH"
BASE COUNT          727 a          768 c          805 g          770 t
ORIGIN      
        1 caccggaact ctatcgaccg ggatggctct tctgagagag gtcgatccca cgttcgaaac
       61 gggtacggcc gaggatatgg ttttcgggag cggattggcg cttttcctcg ggatcccgat
      121 gctcatactt ttgaacattc cgatactcgg ttacagagaa ggcaaaccaa tatactatct
      181 gtacttcctg ctgggtctgg cagcgtatat attggtgctg tacttcttct ggttcaggaa
      241 ggccagaata agaaaacggg aaaaagctaa ttgatgtttc aaaacattaa ctgaaagggt
      301 gattcgatga gaaggttagt aattgtagtt ttgttcgcgc ttcttgcgat cgcggtcgtc
      361 gcgagcgatt taaggctgtt ttacggcaac ccgcattcac atacatctta ttccgacggc
      421 acgggtacgc ccgaagaggc ctacgaatac ggctcaaaag ttcccggact cgactttctg
      481 gcagttaccg atcacggtta ttacttcgtt cagactcttc ccgacggaag ggacaagctg
      541 accgccacga tcgaagcggc gcagaaagct actacggggt cttttctagc ctttgccggt
      601 ttcgaatgga cggcgaccgg aacgggccat ataaacgttt acggaacgca agactggacc
      661 gacagagtcc aatcggacct gtggcagctg tacgactgga ttatcgaaag gaaggccgtg
      721 ggacagttca atcaccctat cacgatgttt ggagataatt tcaagcagtt ccattacgtt
      781 cctcaggccg atctttatgt gaatctgata gaggtcggaa acggtaactg gtggcaaaac
      841 gagacgatct cgcaagagat gttccaggct taccggctgg ccctgctaaa aggctggcac
      901 ctcggcgcca ctctcggaca ggataaccat aagcccaact ggggcggcgc gaacgattca
      961 agagccgtcg tttacgcttc cggtcttacc caagaggaga tcctcaaagc cttcagcgag
     1021 cgcagagtat atgccagcga ggacagagat atccttctct ccttctcggc tgacagcggc
     1081 ttcatgggcg atatccttca cgatctggag gagatcaacc tcaaagtctc gataaaagat
     1141 gaaaccgggg acgatctgga atttgtcgag atctactcgc gaacgggtct tctcgctcat
     1201 ttcgacgtga acgattcgct cttcgagatg gagctccgtc tggatataga gaccggttat
     1261 gaatacttct tcgtgtacgc gaaggccagg gacggtgaag aagtggtttc ctcgcccatc
     1321 tgggttcaga gaagcgatcc cgttcacctt tacaatccgg ccgttcatcc ctcgaacgtc
     1381 aagccgggag aggaagtgac cctttccttc cagatctcga acacctcctc gcaggaggcc
     1441 tcgaccactc tagaactgcg caatcttcac ggcgtgatcc ttcaggagag ctatttgctg
     1501 acggcctacg agtccaggga ggtcactctc ccgcacacga ccggaaacgg tgacgaaagg
     1561 atagatttct accttgatag cgtgccttat tcgagcgtat ctctcaagtt gagagaggcg
     1621 acctcgctca acgttctcct ggacaggagc cacgtgaatt acgccatgga caggcgagaa
     1681 aagctatcga cgacactcca ggaattcggc cacaaatact cgaccgtcga aaggcttctc
     1741 aagactggag aactggacaa tataaatgtc ttgataatgc cgcttccggg ggcgggcgga
     1801 tccttcgaaa ggctcaaaat gctcatgccc gctcaggccg gtatcatcgc cgatttcgtg
     1861 cggaagggcg gcacgctaat aataaccggt acaggtgaga ccgtgggcca ggatatcata
     1921 gacacgtaca acaagtttct cgcggatctg ggccttcccg tgacttttgg cggggttcag
     1981 tccggcgaga tcgtgtcggt agaaggaatt tccttcgatg gaatctccac gctcgaggga
     2041 gaagccggag aatatccagc gggcgatgga actgtaataa tatacccggg agatcctttg
     2101 acggacaatg tgatagagat gaacgaaaag ctcgtatgga ggctgttcta gcaaacaccg
     2161 gttttttgat agagcgttaa gagccgtccc tgcacaacca tgaacggttc ttagcaggag
     2221 cgtttcaggg cagactgtac aggatgaatt tgaatcgtgc ctgcacgata cgaagtatct
     2281 attagttata tcattattta gtagtgacgg gttttccgga gccggttttt ccgtttccgg
     2341 aaaatccgtt tgtggtgaga atggctttat tgaaggcttt ggcgatctgt gcgaatccag
     2401 ggcttcaatt ttctcgcaat cggttatcag tgctgcttta cagaagattc tacaaggtca
     2461 atgtcctccc tgttcaaccg gtatagttca taaattgcaa ggtttatctc ccgatccagc
     2521 agttctatct tctcttcgag ttcgttcagt tcttcgcgct tcttcctgaa ccagccgttg
     2581 agctcttctt tctgccccag tgtcagtctg gctttctgtt tttcgagctc ccacacgaac
     2641 tggttccagc ccaggcgata gaactgttgc aggtttttgc cggttttttt gagcccgtac
     2701 tctgtggaga tcaattccat gcaactcgag agtctgccgg aaagatctga cttcagatct
     2761 atcagttcct tcgttttcgc ctctatcgat tttccggtcg ggctgtccgg aacgaccacc
     2821 gtaacgtttt tcaggtaagt ccagatgagc tggtagccgt tctgaatctc cgtgcaatgg
     2881 cgcgagatca agtaccaacc cagaatgctg ttcagagtgg cgagcaggac gtaatcttcc
     2941 attccgatgt tccataccgc atcgttggtc aggaattcgc ccctggcatc tatggcgaag
     3001 gccggagaca cctggaattt ctggtaactg atcttcggcc gttcgaaggc ttcgtagtat
     3061 tcaaccgaat
//
LOCUS       DAOOLY010000277         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_38876_length_3060_cov_4.448586, whole genome shotgun sequence.
ACCESSION   DAOOLY010000277 DAOOLY010000000
VERSION     DAOOLY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38876_length_3060_cov_4.448586"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLD15_10980"
     CDS             complement(<1..916)
                     /locus_tag="PLD15_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64478.1"
                     /translation="MKMKAIVVVLLVLATAALGSFFRPDVRPGYGVTGIGWLSDYFAP
                     LKGTNMDTPIYFMDSGVEGPTFLLMGGTHGRELSGTTAALVFIENVVVTKGRVIVMPF
                     SNISGVSIPDETGKALHFIQVETKSGPRFLVYGDRRTDPADQGIPDPMVFKHAQSGFT
                     LEDGTEARNLNRQYPGVADGNPTQQLAYAIIEMIKAEKVDFNLDMHESGTPDKYIDER
                     GNTRSGSSLAYTLVCHPDALEIGAVAIMEIEAFYGVSMKLEESNLGYRGLSHLEIRNA
                     TGSLSFLSETPNPGQDSWRKVYDVINDPD"
     gene            complement(977..1528)
                     /locus_tag="PLD15_10985"
     CDS             complement(977..1528)
                     /locus_tag="PLD15_10985"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPB64479.1"
                     /translation="MKKLLLTFFVVLCFAFVSFAAEILLEEPVIVTSAGQSPGALQFT
                     VVAKMVSLDYKFDKLYKAEDVDMSQFKTFVIVVGASGKGLGAAGIDIEVEINRVLALA
                     REAKDNGLKIVICNLEGASRRGESSDKIVTTLAPLASVYFVKSDANEDGFFTNLSEEA
                     GVPMATFEMTVGLKDVLAQYFGK"
     gene            complement(1552..>3060)
                     /locus_tag="PLD15_10990"
     CDS             complement(1552..>3060)
                     /locus_tag="PLD15_10990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPB64480.1"
                     /translation="GGEGYAVITMADGSKYAIDFRSAAPMMATYEAIAEKGLKLSSIN
                     YTPMGTCVPGVLAGIKEAWLLGATLPLSVLAAPAIELARNGFIVDETFSQTTSDTYER
                     LLGSAFDFLNEGFVWETGSVFKNPELADTIERVVEEGIDTFYTGSLADEIDAFMVENG
                     GFLRKSDLESYRAIVREPLHGTYRGYDLYVPHPPVSGPQFLMVLNVLENYNLSAMSWD
                     DPLAIHLIQQALVLGDVDRKAYISDPEFHDLPYEAFMSKEYAKTRLMLINPGKVFDQP
                     YASYLGDASPFADGSTYEEVLLEELAPVMSSARAVEESPSTTHFSIVDKEGNAVSWTQ
                     TISSFFGTGTYFKGFFFNNEMANFASSYREGDVINLTGGMRPRTTICPTVVMKDGEVR
                     WILGTPGAGRIVSMMVNLVVNLVDFNMPIDQAIRSPKFVGYSSYTDLRMEEGFPQSTL
                     DFLEKILGHKLNIYAYPDLFFGGPNIIAVEENGMMIGAGSLRRGGSASAPEF"
BASE COUNT          682 a          847 c          775 g          756 t
ORIGIN      
        1 aatcgggatc gtttatcacg tcgtagactt tcctccacga atcctggccg gggttgggcg
       61 tctctgaaag gaaggaaaga gagccggtgg cgtttcttat ctccagatga ctgagtcctc
      121 tgtagcccag gttggattct tcgagtttca ttgaaacacc atagaaggcc tcgatctcca
      181 ttatggctac ggcgcctatc tcgagcgcgt cgggatggca gaccagcgta taggccaggc
      241 tgctaccgga ccgggtattg cctctttcgt ctatgtactt atcgggagtg ccactctcat
      301 gcatatccag attgaaatct accttctcgg cctttatcat ctcgatgatc gcgtaggcca
      361 gttgctgggt cgggttgcca tcagccactc ccgggtactg tctgttcagg ttccttgctt
      421 ccgttccgtc ttccagcgtg aaacccgact gtgcgtgctt gaaaaccatc gggtcgggta
      481 tcccctggtc ggccggatcg gttcttctgt caccatacac cagaaagcgc gggccggatt
      541 tagtttcgac ctgaataaaa tgcagcgcct tgccggtttc atcgggaatg gagacaccac
      601 ttatattgga gaagggcatc acgatcaccc tacccttcgt gacgacgacg ttctctatga
      661 agaccagagc ggccgtagta ccggaaagct cccttccgtg tgttcctccc atgagaagga
      721 aggtagggcc ttcaacaccg ctatccatga agtagatggg cgtatccatg tttgttcctt
      781 ttagaggtgc gaaataatca gacagccagc caatacccgt cacgccgtaa ccgggcctca
      841 catcgggcct gaaaaacgaa ccgagagcgg ccgttgccag aaccaataat actactacta
      901 tcgctttcat cttcattctt tgtctttcct ccgatctaaa aataaatggg gcgtgcggag
      961 acgccccatt tttcaattac ttgccgaaat actgcgccag tacgtccttc aggcccaccg
     1021 tcatttcgaa tgtggccatg ggtactccgg cctcctcact caaattggtg aagaaaccgt
     1081 cttcgttggc atcggactta acgaagtaaa cgctggcgag aggtgccaga gtcgtgacta
     1141 ttttatccga cgattcgccc cttctcgacg ctccttcgag gttacagatc actattttca
     1201 gtccgttatc tttagcctct cttgccagtg ccagaactct gttgatctcg acttcgatat
     1261 cgattccggc tgctccaagc cccttgccac tcgcgccgac gacgatgacg aaggtcttga
     1321 actgactcat atcgacatct tcggccttgt acagtttgtc gaacttgtag tccaggctca
     1381 ccatcttggc gacaacggtg aactggagag ctcccgggga ctggccggcc gatgtgacaa
     1441 tcacgggctc ttcaaggagt atttccgccg cgaaactcac aaacgcgaag cacaaaacaa
     1501 caaaaaacgt aagaagtaac tttttcaatt caaatcatcc ctttctcatt ttcaaaattc
     1561 cggtgcggaa gccgaaccac ccctcctcag ggaaccggct ccgatcatca ttccgttttc
     1621 ctcaacggca attatgttcg ggccgccgaa gaagaggtcg gggtaagcgt agatgttgag
     1681 tttatgaccg agaatcttct ccaggaagtc cagcgtggat tgcgggaaac cctcttccat
     1741 tctcagatcg gtgtaggagg agtacccgac gaacttgggg gaccttatcg cctggtcgat
     1801 gggcatgttg aaatccacca ggttaaccac cagattgacc atcatcgaaa ctattctgcc
     1861 ggctccaggc gttcccaata tccagcggac ctctccgtcc ttcataacaa ccgtcggaca
     1921 gatcgtcgtt ctcggtctca ttcctcctgt gaggttgatt acgtcgccct ctctgtaaga
     1981 agaggcgaag ttggccattt cgttgttgaa gaagaaaccc ttgaaataag tcccggtgcc
     2041 gaagaagctg gaaatcgtct gtgtccagga gacggcgtta ccttccttgt caactatcga
     2101 gaagtgcgtc gtggacggag actcttctac cgctcttgca ctggacatga ccggggccag
     2161 ttcctccaga aggacttcct cgtaggttga tccatcggcg aagggggagg catcgccgag
     2221 atagctggcg taaggttggt cgaaaacttt tcccggattg atcaacatca gtctggtctt
     2281 tgcgtactct ttgctcatga aggcttcata aggaagatcg tggaactccg gatcgcttat
     2341 gtaagccttc ctatcgacgt cgccgagaac cagtgcctgc tggatcagat gaatagccag
     2401 cggatcgtcc cagctcatcg ccgacaggtt gtagttctcg agcacgttga gaaccattaa
     2461 aaattgaggc ccggagactg gcggatgtgg aacgtacagg tcgtatcctc tgtaggtgcc
     2521 gtgtagaggc tctctcacga tcgcgcggta ggactcgaga tcggacttcc tcaagaagcc
     2581 accgttctcg accatgaagg cgtctatctc gtctgccagc gaaccggtgt agaaggtgtc
     2641 tatgccctct tcaacaactc tctcgatagt gtcggccagt tcagggttct tgaaaacgct
     2701 tcccgtttcc cagacgaatc cttcgttgag gaagtcgaat gcgcttccga gaagtctttc
     2761 gtacgtgtcc gaggtagtct gagaaaaggt ctcatcgact atgaacccgt tcctggcaag
     2821 ctctatggcc ggggccgcca gaaccgacag aggaagtgta gcccccagaa gccaggcctc
     2881 tttgataccg gccagaactc cgggaacgca tgtccccatg ggagtataat tgatcgagct
     2941 cagcttcagc cccttttcgg cgatcgcttc gtacgtagcc atcatcggcg cggcgcttct
     3001 gaaatcgatc gcgtatttgc tgccgtctgc catcgttata acggcgtaac cttcgccacc
//
LOCUS       DAOOLY010000278         3057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_38920_length_3057_cov_4.851432, whole genome shotgun sequence.
ACCESSION   DAOOLY010000278 DAOOLY010000000
VERSION     DAOOLY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38920_length_3057_cov_4.851432"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLD15_10995"
     CDS             complement(<1..490)
                     /locus_tag="PLD15_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HPB64481.1"
                     /translation="MYQTILEVKNLKKYFYISKSQVLKAVDNVNFSIERGKILGMVGE
                     SGCGKTTVGRTIMHLYRPDEGEVLFNGIDVHQLNNKKYRQITKKMQMIFQDPYASLDP
                     RKTVGSIIGEGIDIHRLCRTRSERMERIYQMLEMVGLNREHANRFPHEFSGGQRQRIG
                     IAR"
     gene            complement(492..1535)
                     /locus_tag="PLD15_11000"
     CDS             complement(492..1535)
                     /locus_tag="PLD15_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64482.1"
                     /translation="MSDKILEVNDLEYSFDTYAGEVHAIRGVSFYVNKSESLAIVGES
                     GCGKSVTVQSIMKLVPTPPGRFKAGKIIYCGNDITDYGDRQMEKLRGKEMSMIFQDPM
                     TSLNPTMRIGRQICEGIMKHEKISAAEAMKRSVKLLKEVGIHDPEKNVMRYPHMFSGG
                     MRQRAMIAIALACNPKILFADEPTTSLDVTTQAQILEMMNHLKRELKMAIVLITHNLG
                     IVARFAERISVMYAGKIIESGTTDDIFYSPRHPYTWGLLSSVPAVGKKRTEKLSTIKG
                     TPPDLFMPPIGCGFYDRCDYRMKVCKENDPPSREIGDSHWASCWLLDARAPGVKPYVV
                     KRHSGESSCKDVM"
     gene            complement(1550..2476)
                     /locus_tag="PLD15_11005"
     CDS             complement(1550..2476)
                     /locus_tag="PLD15_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64483.1"
                     /translation="MIKDANIPHDEFEFVQRDEGKSEALTRPSISFWKDVWRRLLKNK
                     VAMGGLIIILTIALLSIIIPEVSSYSYSQQNLKNINAAPSPEHWFGTDSLGRDLWVRT
                     WVGARVSLAVGIFGSIIPSLIGIVIGGISGYFGGKVDMIIMRLIDIIICIPQMIYVIL
                     IMLVIGSGPVPLILAFAITGWMGSARNVRGLILKIKEEEFVLASRILGASHGYIIFKH
                     LVPNTLGIVVVSMTLGVPAAIFQEAYLSFIGLGIKPPIPSWGQLANLGVSVFRIYPSQ
                     LLIPAIMISLTMLSFNLFGDGLRDALDPKLRR"
     gene            complement(2487..>3057)
                     /locus_tag="PLD15_11010"
     CDS             complement(2487..>3057)
                     /locus_tag="PLD15_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64484.1"
                     /translation="VSARKRGKAVDYLCVVVSVIGVSIPSFILGSFLQFVFALKLKVL
                     PMAQWTTEMHKILPTVAIALGLLATLTRIMRASMLEVTTQDYVKTAKSKGLSEGKIVW
                     SHEIRNALIPILTMLGPMVASVLMGTFVIEKIFAIPGLGLHFVNSITGLDYTMTMGLT
                     VFFGAFLVTANFLVDIAYGVVDPRIRIAK"
BASE COUNT          706 a          839 c          742 g          770 t
ORIGIN      
        1 cccgcgcgat cccgatgcgc tggcgctggc cgccgctgaa ctcgtgtggg aagcggttgg
       61 catgctcgcg attgagaccg accatctcca gcatctggta tatccgttcc atgcgttccg
      121 atctggtacg gcacagtctg tgaatatcga tgccctcacc gattattgag ccgacggtct
      181 ttctcgggtc gagcgaagcg tagggatcct gaaagatcat ctgcattttt ttcgtgatct
      241 gccgatattt tttgttgttc agctggtgga catcgatccc gttgaagaga acttccccct
      301 cgtcgggcct gtaaagatgc atgatagtcc ttcccacggt ggtctttccg cagccggact
      361 cgccaaccat tccaagtatc tttccccgtt ctatagagaa gtttacgtta tcgacggcct
      421 ttagaacctg gctcttggat atataaaagt attttttaag gttcttgact tcgagaatag
      481 tttgatacat ctcacatcac atccttgcag gaactctctc cggagtgcct cttcaccacg
      541 tacggtttca cgcccggagc cctggcatcc agaagccagc aggaagccca atggctatcc
      601 ccgatctccc tggacggcgg gtcgttctcc ttacagactt tcatacgata gtcacagcga
      661 tcgtagaagc cgcagccgat aggcggcatg aagagatccg gaggagtccc tttgatcgtc
      721 gaaagctttt ccgttctctt cttccctacg gcgggaacgc tgctcaaaag cccccacgtg
      781 tacgggtgac gcggagagta aaagatgtca tccgtcgttc cgctttcgat gatctttccg
      841 gcgtacatta ccgaaatacg ctcggcgaat ctggcaacta ttccgaggtt gtgagtgata
      901 agaactatgg ccattttcaa ctcccgtttt agatggttca tcatttccag aatctgtgcc
      961 tgcgtggtga cgtccagaga tgtggtcggc tcgtcggcga agagtatctt tggattgcag
     1021 gccagtgcga tcgctatcat ggctctctgt ctcattcccc ccgaaaacat gtgaggatag
     1081 cgcatgacgt tcttctctgg gtcgtgtatg ccgacctctt tcagtagctt tacgctcctc
     1141 ttcatcgctt cggcagcaga gattttctcg tgcttcatta tcccctcaca tatctgtctc
     1201 ccgatccgca tggtagggtt aaggctggtc atggggtcct ggaaaatcat cgacatctcc
     1261 ttccccctca acttctccat ctgcctgtcg ccgtaatcgg taatatcgtt gccgcaatag
     1321 attatcttgc ctgccttgaa ccttcctgga ggtgtcggga caagcttcat gatcgattgt
     1381 accgtgacgc tctttccaca gccggactct ccaactatcg caagcgattc actcttgttc
     1441 acgtagaagg atacgcccct aatagcgtgg acttcaccgg cgtaggtatc gaaggagtat
     1501 tccagatcgt tgacttccag tatcttatcg gacatcgcaa aatccccctt catcgacgca
     1561 atttcggatc gagcgcatcc ctgaggccgt ctccgaaaag gttgaaggaa agcatcgtca
     1621 gggaaatcat gatcgccggt atgagaagct gtgacggata gatcctgaag acagaaacgc
     1681 caagattcgc cagctgtccc cagctgggta taggaggttt tattcccagc cctatgaaac
     1741 tgagataggc ctcctggaag atggccgcag gaacgcccag cgtcatgctc acgacgacga
     1801 tccccagtgt gttcgggacg aggtgcttga aaatgatgta gccgtgcgag gcgccaagta
     1861 tacgcgaagc cagcacgaac tcctcttcct tgatcttcag gatgagaccg cgaacgttgc
     1921 gggccgaacc catccagccg gttatcgcga aggccagtat gagtgggacg ggaccggagc
     1981 cgatcaccag cataatcagg atcacataga tcatctgagg aatacagata attatgtcaa
     2041 tgagtctcat tattatcata tccaccttgc cgccgaagta acccgatatg ccccctatca
     2101 ctattcctat gagacttgga attatcgaac cgaagatccc caccgcgaga gatactcttg
     2161 cccccaccca ggtccgaacc cacagatctc ttccaagtga atcggtaccg aaccagtgct
     2221 ctggcgaagg tgcagcgttt atgtttttaa gattttgctg gctgtaagaa tacgacgaca
     2281 cctctggaat aattatcgag agaagcgcta tcgtcagaat gattatgagt ccgcccatcg
     2341 ctactttgtt ctttagaagt cttctccaga cgtccttcca gaatgaaata ctgggacggg
     2401 tcagagcctc cgattttcct tcatctcttt ggacgaattc gaactcgtcg tgtggtatat
     2461 tcgcgtcttt tatcatggca gatacctcac tttgcgattc tgatacgcgg gtcaactacc
     2521 ccgtaagcga tatcgaccag gaagtttgca gtaaccagga aggccccgaa gaagacggtc
     2581 agccccatcg tcatagtgta atccagaccg gtaatcgagt tgacaaaatg caaacccagc
     2641 cccgggatcg cgaagatctt ctctatcacg aaagtcccca taagcacact ggcgaccata
     2701 ggccctagca tggtgagtat gggaatcaga gcgtttctga tttcgtggct ccagactatc
     2761 tttccttcgc tcagaccctt cgatttggcc gtcttcacgt aatcttgagt cgtgacttca
     2821 agcatgctgg ccctcatgat tctggtgagc gtcgccagta atccaagagc gatagcgacc
     2881 gtcggaagga tcttgtgcat ctcagtagtc cactgggcca tcggtagcac cttgagcttc
     2941 aacgcgaaaa cgaactgaag gaaggagccg aggatgaagc tcggtatcga aacacctata
     3001 accgaaacca ccacacacag gtaatctacg gccttccccc gctttcttgc agacacg
//
LOCUS       DAOOLY010000279         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_39194_length_3041_cov_4.911587, whole genome shotgun sequence.
ACCESSION   DAOOLY010000279 DAOOLY010000000
VERSION     DAOOLY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39194_length_3041_cov_4.911587"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..238
                     /gene="rpmG"
                     /locus_tag="PLD15_11015"
     CDS             71..238
                     /gene="rpmG"
                     /locus_tag="PLD15_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPB64485.1"
                     /translation="MAKKTKGNKALVTLKCSVCGTRNYYRFKNRQKKYKLDSNKFCPK
                     CRKHTEHKESK"
     gene            273..479
                     /gene="secE"
                     /locus_tag="PLD15_11020"
     CDS             273..479
                     /gene="secE"
                     /locus_tag="PLD15_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPB64486.1"
                     /translation="MAKARFWKFLSEVKSEVKKVTWPNRQQMISSTGAVLVILIVSGV
                     FLGLLDVLFTNVIGSLLSFLTGGA"
     gene            505..1569
                     /gene="nusG"
                     /locus_tag="PLD15_11025"
     CDS             505..1569
                     /gene="nusG"
                     /locus_tag="PLD15_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPB64487.1"
                     /translation="MRKKWFIIQTYSGLENSIREAIQTKIESFGFSHLFGKILVPEET
                     KLDRANAAAEKHIIPANARLLVKENQDVAKGEPVAEELEIKVKNDGAIAEVKNYRVIF
                     IETADRRYTKTYYIPESAKIETGVKTGARIRQGMPLAKSGEYFCELDGKIVYTQKMKR
                     VVIERVNGEEDVYLIHPDSCDMRLVKRGTAVKRGDVLGDSRKVTSKTEGRIELSELPG
                     RKEIKIFKIIRTRLYPGYVFIEMIMNEETLNLVKNTPNVVNFVSVGGQPVELKGKEVK
                     ALLRLVGIEDYEEHVGPIKIEVEFEIGEIVRINSGPFEDFVGKITDLDPERQELKVVV
                     SIFGRETPVILGLSEVEKIV"
     gene            1616..2041
                     /gene="rplK"
                     /locus_tag="PLD15_11030"
     CDS             1616..2041
                     /gene="rplK"
                     /locus_tag="PLD15_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPB64488.1"
                     /translation="MAKKILAQVKLQLEAGKATPAPPVGPALGQHGVNIMGFCKQFNA
                     ETADRAGMILPVIITVYADRSFVFELKTPPASFLLLRAAGIQKGSGIPNRTKVGKITR
                     AQLEEIAKVKMPDLNARTVDAAAKIIAGTARNMGIEIVG"
     gene            2054..2752
                     /gene="rplA"
                     /locus_tag="PLD15_11035"
     CDS             2054..2752
                     /gene="rplA"
                     /locus_tag="PLD15_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPB64489.1"
                     /translation="MPVRSKRYTQARQLVDRMKTYSVEEGIEILKKFPSAKFNETVEM
                     HLKLGIDPAKSDQQVRGTISLPHGTGKDIRVLVFARGEQAEAAKAAGADFVGSDELVQ
                     QIQGGWTDFDVAIATPDIMRDIGRLGKVLGPRGLMPSPKAGTVTTDVVDAVKGFKAGR
                     IEVKNDKTGNLHLPVGKKSFESQKLLENFVSALNQINRMKPSGSKGRFVQRAFITTTM
                     GVGIKIEISRETEK"
     misc_feature    2774..2897
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2906..>3041
                     /gene="rplJ"
                     /locus_tag="PLD15_11040"
     CDS             2906..>3041
                     /gene="rplJ"
                     /locus_tag="PLD15_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPB64490.1"
                     /translation="MLTRARKEEIVQDLTDRYQRASLILFAEYKGMKVDAITQFRDKL
                     Y"
BASE COUNT          883 a          672 c          838 g          648 t
ORIGIN      
        1 cggaacgcct tcggtggttc aaacccaccc cttaccacca ttaattttgc tacttgttga
       61 ggtgcatgca atggcaaaga aaacgaaggg aaacaaggct ctggtgactt tgaagtgttc
      121 ggtctgtgga acgagaaact actacaggtt caagaatcgc cagaagaagt acaagcttga
      181 ctcgaacaaa ttctgcccca agtgcagaaa gcataccgag cacaaagaat ccaagtaggt
      241 tcgggacgat ctgttgaaat aggaggaatc ccatggccaa ggcaaggttc tggaaatttc
      301 tttccgaagt gaaaagtgaa gtaaagaagg ttacatggcc taacaggcag cagatgatct
      361 catcgactgg tgccgttctc gtgatcctta tcgtcagcgg agtattcctc gggctcctcg
      421 atgtgctctt cactaacgtt atcggaagtc ttttgagttt tctcaccggt ggcgcttgat
      481 acgcccatgt gctggtgata tgtagtgcgc aagaagtggt tcataattca aacttattcc
      541 ggtctggaaa actctatcag agaggctatt cagactaaga tagagtcttt cggtttttcg
      601 cacctcttcg gtaaaatact ggttccggaa gaaactaaac tggacagagc caacgccgcg
      661 gcggagaaac atataattcc tgccaacgcc aggttgcttg tgaaggagaa ccaggatgtc
      721 gccaagggag aaccggtcgc ggaagaactt gagataaaag tcaagaacga tggagctata
      781 gcagaggtca agaattacag ggttatcttc atcgaaacgg ccgacaggcg ttacacaaag
      841 acttattaca tacccgaaag tgctaagatc gagaccgggg ttaagaccgg tgcgaggatc
      901 agacagggta tgcctcttgc gaagagcgga gagtacttct gcgaactcga cggtaaaata
      961 gtttacaccc agaagatgaa acgcgtggtc atcgaacgag tgaacggcga agaagatgtc
     1021 tatctcattc atcccgacag ctgcgatatg aggttggtga agcgcggcac ggctgtgaag
     1081 aggggagacg tgctcggtga ttccaggaag gttacctcca agaccgaggg caggattgag
     1141 ctgtcggaac ttcccggcag aaaagagata aagatcttca aaataatcag gacgagactt
     1201 tatcccggtt atgtattcat agagatgata atgaacgaag aaacgctgaa cctggtgaag
     1261 aacacgccca acgtcgtcaa cttcgtctcc gttggaggtc agcctgtgga actgaagggc
     1321 aaggaagtga aggcgcttct caggctggta ggcatagaag attacgaaga acatgttgga
     1381 ccgatcaaga tcgaagtcga attcgagata ggcgagatag tgaggataaa ctccggtcct
     1441 ttcgaggact tcgtaggcaa gatcaccgac cttgaccccg aaaggcagga gctcaaggtt
     1501 gtagtgagta tcttcggaag ggagaccccc gtaatcctcg ggctttccga agtcgaaaag
     1561 atagtttgaa gtagtgggag ggctcaggcc cgatagacca cagggaggtt tttcaatggc
     1621 aaagaagatt ttggctcagg ttaaactaca acttgaggcc ggtaaggcaa ctcccgcacc
     1681 accagtcggt cccgctctgg gtcagcacgg tgtgaacatc atgggttttt gtaagcagtt
     1741 caacgctgaa accgctgaca gggccggtat gatacttccc gtaatcatca cggtttatgc
     1801 cgacagatcc tttgtgtttg aactgaagac tccgcccgcg agtttccttc tcctcagggc
     1861 cgccggaatt caaaaaggtt ccggaatacc caacaggaca aaggtcggaa agatcaccag
     1921 agcccagctg gaggagattg cgaaagtgaa gatgcccgat ctcaacgcca gaacagtaga
     1981 tgccgcagca aagataatcg ccggaacggc gcgcaacatg ggcatcgaaa tagtaggttg
     2041 aggggggatc tgaatgccgg ttagatctaa gagatacaca caggcgaggc agcttgtgga
     2101 caggatgaag acttattcgg tggaagaggg tatagagatt ctcaagaagt tcccttccgc
     2161 caagtttaac gaaaccgtgg agatgcacct caagcttgga atagatccgg caaaatccga
     2221 tcagcaagtt cgcggaacta tatcgcttcc ccacggaacc ggaaaggaca ttagggtact
     2281 ggttttcgcc aggggtgaac aggccgaagc ggcaaaagcg gccggcgccg atttcgtggg
     2341 ctccgatgaa ctggtacaac agatccaggg tggctggacg gacttcgacg ttgcgatcgc
     2401 cacacctgat ataatgaggg acatcggtag gctcggtaag gtgctcggtc caagaggctt
     2461 gatgccctct cccaaggccg gaacggttac aaccgacgtc gtcgatgctg taaagggatt
     2521 caaagctgga agaatcgaag tcaagaacga caagactgga aaccttcatc ttccagtagg
     2581 caagaagtct tttgagtctc aaaagctcct ggagaacttt gtatcggctc tgaaccagat
     2641 aaacaggatg aagccttccg gttccaaagg gcgtttcgta cagagagcct tcatcaccac
     2701 aactatggga gtcggtataa agattgagat ctcaagggaa acagagaagt agaaataata
     2761 gcaagaaaca taagaaagat aacctgcggt accgaagaca gtaggtattt aagagccaat
     2821 cgccgcctac cgaggtacgt ggaagttata ttaccttctg cggcctcgat tcatggggcc
     2881 gcttttttga aaggaggtgc gagaaatgct taccagagcc agaaaagaag agatcgttca
     2941 ggatctgacc gaccgctatc agagggcctc tcttatactt ttcgctgaat acaagggaat
     3001 gaaagtcgat gcgataaccc agttcagaga caagctttat g
//
LOCUS       DAOOLY010000280         3040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_39210_length_3040_cov_5.088442, whole genome shotgun sequence.
ACCESSION   DAOOLY010000280 DAOOLY010000000
VERSION     DAOOLY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39210_length_3040_cov_5.088442"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /gene="murC"
                     /locus_tag="PLD15_11045"
     CDS             complement(<1..1314)
                     /gene="murC"
                     /locus_tag="PLD15_11045"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPB64491.1"
                     /translation="MKYHFIGIGGIGMSGLAMHLASEGYQVYGSNYEENERVEYLRAR
                     GIGVCIGHSSENFDRPDLVIRTTAIKQGNPELVRAISEDVPTIYRMELLQKILAGNIS
                     TCITGTDGKTTTTAMVAKILFDDGRDPTVFLGGLNSFLTDGNYRRGGNVIVSELDESD
                     GFFASFKPENAIITNVRGDHLEHYDNSFDNLKNHFRYFSRGVKNLLVFNADDPVSERL
                     FKGGITFGKEKGLYRFSNRKTCLMSQTFSCWRGDSHMGQIKLMIPGEYNAYNATAAVA
                     LTHELGVSIDTIKASLESFRSVDRRFTFRGMDNSRNLFFFDDYAHTPDEISSTIRGAR
                     EFFPDKNVIVVFQPHRYSRLVRENGRFALSLRDASEVCVYKLYEAYEKGQYAIDETEV
                     LKGLSSYGVPAVHAVNYNEILDWLERKRDAVVLFLGAGDITEASKL"
     gene            complement(1311..2381)
                     /locus_tag="PLD15_11050"
     CDS             complement(1311..2381)
                     /locus_tag="PLD15_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPB64492.1"
                     /translation="MRRLKIAFCGGGTGGHYYPAVALLQEITRRTDFDLLYFTVKGKI
                     DDRSVERDFPGVSRIPLELTGLLRPLYRPGNIPILFSHMMAKKQVIRSLRDFRPDFLF
                     STGGYVSFPVIKAAHELEIPVFIHEQNSIAGITNKKMARYAEYFFISFEETAGEVKLP
                     AARVVLSGNPVRESTKSREEVLEGFGFSCEFPFVVVLGGSLGSSVINDACEKLYVLLE
                     ESNTRMNFLHSTGDEEKAKDLLRFSFVRSFAYIENLSDAIAVSDLVISRAGATTIAEL
                     QYHGRKGIIIPWPGAAENHQLYNAISLERLGLGYVILESDLTPEKIFYATQEMLNRNI
                     GYMPPRRPVDIILDYILREESI"
     gene            complement(2368..>3040)
                     /locus_tag="PLD15_11055"
     CDS             complement(2368..>3040)
                     /locus_tag="PLD15_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB64493.1"
                     /translation="FLVFIEPDLSTTGILVAITFVMMIVGGIKLRYILLAIVLGVLII
                     LILYSGGFIKPYQLERITSFLTSITGEEHEQVSYSIMAISSGGLLGKGLGMGLVKYYL
                     PVSYSDFIFAVIGEELGLIGIVVLMMAYVGFIRELILAGLKGPSKLEGKLYIIGFALY
                     IMIQATINIAVNLGLFPPTGVTLPFVSSGGSSIMSLMIGYGLVFAILIEKEEENKASE
                     THEKT"
BASE COUNT          760 a          789 c          690 g          801 t
ORIGIN      
        1 cagctttgat gcttctgtta tatctcccgc acccagaaag aggacaacgg catccctttt
       61 tctttccagc cagtccagta tctcgttgta gttaaccgcg tggaccgcgg ggactccgta
      121 actgctgagt cccttcaaga cctcggtttc gtcgatcgcg tactgaccct tctcgtaagc
      181 ttcgtagagt ttatagacac agacctcgct tgcatctctc aaagagagcg caaagcggcc
      241 attctccctc acgaggcggg agtacctgtg cggctgaaag accacgatta cattcttgtc
      301 tggaaagaac tccctggcac cccttatggt gctgcttatc tcgtctggag tgtgggcgta
      361 atcgtcgaag aaaaagaggt tcctgctgtt gtccatacct ctgaacgtga atcttctatc
      421 aacagacctg aaagactcca gggaggcttt gatcgtgtct atcgacactc caagttcgtg
      481 ggtcagcgca accgccgctg tcgcgttgta ggcgttgtat tcacctggta tcatcagctt
      541 gatctgtccc atatggctgt cgccgcgcca acagctgaag gtctgactca tcagacaggt
      601 ctttctattg gaaaaacgat acaacccttt ttctttgcca aaggtgattc cgcctttgaa
      661 aagcctttcg gaaaccggat catcggcgtt gaataccagg aggttcttta cccccctgga
      721 gaagtacctg aaatggtttt tcaggttgtc gaaggaatta tcgtagtgct caagatgatc
      781 gcctctgacg ttggtaatta tagcgttctc aggtttgaaa gaggcgaaaa agccgtcgct
      841 ctcatccagc tcgctaacga taacgttgcc acctctcctg taattaccgt cggtgagaaa
      901 ggaattcaac ccgcccagaa agaccgtagg gtccctcccg tcatcgaaga gtatcttggc
      961 aaccatggcg gtggtggtcg tcttgccgtc ggtaccggtg atgcatgtag atatattgcc
     1021 ggcgaggatc ttttgtaaca attccattct gtaaatagtg ggaacatctt cggagatagc
     1081 ccttacgagt tcgggatttc cctgttttat cgccgtagtc cttatcacaa gatctggtct
     1141 gtcgaaattc tcggaagaat ggcctataca aacacctatc ccccgggctc taagatattc
     1201 taccctctcg ttctcttcgt agttcgaacc gtatacctgg tatccctcag atgctaaatg
     1261 cattgccaga ccgctcattc ctattccgcc aatgccaatg aaatggtatt tcaaatcgat
     1321 tcctcccgga gtatataatc caaaataata tcaacaggtc ttcttggagg catataaccg
     1381 atatttctgt tcaacatctc ttgtgttgca tagaaaattt tttctggggt taggtcactt
     1441 tcgagtataa cataaccgag accaagtctc tccagtgaaa tcgcgttgta caattggtga
     1501 ttttctgccg ctccaggcca cggtattatt ataccctttc taccgtgata ctgtagctcg
     1561 gcgatagtgg tcgcaccggc cctggaaatt acgagatccg aaacagctat cgcatcggaa
     1621 agattctcta tataggcaaa agacctcacg aaagaaaaac gcagcaaatc cttcgctttt
     1681 tcttcatctc ccgtcgaatg cagaaagttc atacgcgtat ttgactcttc aagcaggaca
     1741 tacaactttt cgcaggcgtc gtttataacc gaagatccga gactgccccc gaggacgacg
     1801 acgaagggaa attcgcagga aaaaccgaat ccctccagta cctcctctct cgatttggtg
     1861 ctttctctga ccggatttcc cgaaagtaca acgcgtgctg ccggtagctt cacttcaccg
     1921 gcggtctctt cgaaagagat aaaaaagtat tcagcgtatc tggccatctt tttgttggtt
     1981 atgccggcga tcgaattctg ttcatgtatg aataccggaa tttccagttc gtgagccgct
     2041 tttataacag ggaaggaaac gtaaccgcca gtcgaaaaga ggaaatcggg tctaaaatct
     2101 ctcagacttc taataacttg ttttttggcc atcatatgag agaagagtat cgggatgttt
     2161 cccggtctgt aaagcggcct caaaagtccc gtgagttcca gcggtatccg tgaaaccccc
     2221 ggaaagtctc tttccacact cctgtcgtct atctttccct ttaccgtgaa gtacagtaaa
     2281 tcgaagtcgg tgcgcctggt tatctcctga agaagagcga cggccggata ataatgcccg
     2341 ccggtgccac cgccacaaaa ggctatctta agtcttctca tgtgtttcgc tcgccttgtt
     2401 ttcttcttct ttctctataa gaatcgcgaa taccaggccg taaccgatca tcagcgacat
     2461 tatcgaagag cctccagagc tgacgaaggg aagggtgacc cccgtcgggg gaaagaggcc
     2521 cagattgacc gcgatgttta tcgtggcctg aatcattata tacagcgcaa aacctattat
     2581 gtacagcttt ccttccagtt tcgacggccc cttgagtccc gctagtatca actcccttat
     2641 gaaacccacg taggccatca tgagtaccac gatccctatg agacccagtt cttcaccgat
     2701 cactgcgaag atgaaatccg agtaactcac cgggagatag tacttcacaa gtcccatgcc
     2761 aagaccttta cccagcagtc cgcccgaaga gatggccatg atcgaatagg aaacctgttc
     2821 gtgctcctcg ccggttatag atgtgaggaa cgaggttatt cgctccagct ggtagggctt
     2881 gataaatcct ccgctgtaaa gaattaggat gatcagtacc cccagcacta tggcgagtaa
     2941 tatgtagcgc agctttatac ctccgacgat catcatcacg aaggttatgg ccacgagaat
     3001 accggtcgtc gacagatcgg gctcaatgaa aacaagaaag
//
LOCUS       DAOOLY010000281         3015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_39696_length_3015_cov_3.624662, whole genome shotgun sequence.
ACCESSION   DAOOLY010000281 DAOOLY010000000
VERSION     DAOOLY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39696_length_3015_cov_3.624662"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..275)
                     /locus_tag="PLD15_11060"
     CDS             complement(3..275)
                     /locus_tag="PLD15_11060"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPB64494.1"
                     /translation="MVETAGLDILLLMPQGIALRSLKALREEGVAAKLFPTPPQRFPG
                     CSISIAVPSGNLEFCLELLERIGLSPLSIFHCSSNPVREFYEDTGN"
     gene            complement(268..>3015)
                     /gene="recJ"
                     /locus_tag="PLD15_11065"
     CDS             complement(268..>3015)
                     /gene="recJ"
                     /locus_tag="PLD15_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPB64495.1"
                     /translation="RGHSLLITVDCGVTSFGEIEYAREIGFDVVVTDHHEVKDILPPA
                     NAVINPKRPDDSYPFKGLAGVGVAFKLLVALNETLRCPINPEDYLDIVALGTIADIVP
                     LCDENRYIVREGTAKIQSSPLLGLKALLSYLRIPSENLMAQDIAFKIAPKLNAAGRMD
                     SAIVALELLISEDMDSAMNTASRLLKHNQNRQTIEAKIFEQTSKDLEINHRMSNDFAL
                     VIDGDSWHLGVLGIVASRLVSIHNKPVFLISTNGQDGKGSARSPSGVSIISLLNEVSS
                     LLREFGGHEMAAGFSIGKENIPEFRKAINEAYIRQYGRKLPVFKIDVDDVLSLDSITP
                     HVLDKLEILRPFGHSNPEPKFLIKGLNIEKAKTFGNGSDHVKMVLRSGDRKTLAIGYN
                     MSSMFDDFKYVKPNLLKVDAVASIKSDNLYGLQSVKLSLSDVNLYIDPVFEEEVRDKN
                     FVFEFIRDWKNQQPASQVKNDVSALISELEKKLSHKCPEFMDVSSKSPWGAFGNIRLK
                     NPFLALRILKNYQKGLKTFIVSAINGTLAHTYYSLQHYLDAIKPVFANSLYRGNLEES
                     VIFVTLPFFVENFAKISETAGEIIFDEPTYIISGIFKGHPDLDSFLGLIDSVIDISGF
                     TGSVYYDDLKDFLADRRISHVYKPNPIKKVGIIDNRGSRKKVEQVMSIVGHGENVAVI
                     VDSPHKTVTLAKSMGSRLSHALQNGELIFYNYMLKDFQRSVIYSLVERQKIRVLITTP
                     SSDGLGVTLGNSNIVFYSAPRNFLEVLDSVSTRPGEDSELFLNLSFNKSDLLSNTSEM
                     DRLFPTIEELEAVYQDLKDVLPAPERDIRKALSFEDGISKVYLSILEEMNLLNYDGNV
                     WYSSSNGELDTSLVKKTLRYREGIAEKRMTRWFATKLSTMTTRGLLRSLTNSEEVLRV
                     G"
BASE COUNT          698 a          746 c          749 g          822 t
ORIGIN      
        1 aatcaattcc cagtatcttc ataaaactct ctcacgggat ttgaagagca gtgaaaaatg
       61 gagagtgggc tcagaccaat tctctccaga agctcgaggc aaaattccag attgccggaa
      121 ggaacggcga tagatatgga acagcccgga aaacgttgcg gcggggtggg aaagagtttg
      181 gcggcaaccc cctcttccct caacgctttc agagaacgga gagcgattcc ctgaggcatt
      241 aaaagcagga tgtccagacc cgctgtctca accaactctc agtacctctt cggagttggt
      301 cagactgcgt aacagtcccc tggtggtcat agttgaaagc tttgtggcga accagcgcgt
      361 cattctcttc tcggctatac cctctctgta tctcagggtt tttttgacca gagaagtgtc
      421 cagttctcca ttcgaactgc tgtaccacac gttgccatcg taattcagca ggttcatctc
      481 ttccagaatc gaaagataga ccttggagat cccgtcctcg aagctcaaag cctttcttat
      541 atctctttcg ggagcgggga gcacgtcctt caaatcctga tagacggctt ccagttcttc
      601 gatagtgggg aaaagcctgt ccatttcgct ggtgttcgag aggagatcgc ttttgttgaa
      661 cgagaggttc aagaaaagtt cggagtcttc acccggcctt gttgagacgg aatcgaggac
      721 ctctagaaag tttctcggag cgctgtaaaa tacgatgttg gagtttccca gcgtgactcc
      781 cagtccgtcg ctggagggcg tggtgatcag aacccttatc ttctgccttt cgaccagcga
      841 gtagatcacc gatctctgga agtccttcaa catatagttg tagaatatca gctcaccgtt
      901 ttgaagggcg tgagaaagcc tcgaacccat cgatttggcc agcgtgaccg tcttgtgtgg
      961 agaatcgacg ataacggcca cgttttctcc atgaccgacg atagacatga cttgctcgac
     1021 ctttttccgg gagcccctgt tgtctatgat gcctaccttt ttgatggggt tgggcttata
     1081 cacgtgggag attctcctgt cggccaggaa gtctttgaga tcatcgtaat acacgcttcc
     1141 ggtgaagccc gatatgtcta tgaccgaatc tatcaagccc agaaagctgt cgaggtcggg
     1201 atgtcccttg aatatacccg agataatgta ggttggctcg tcgaatataa tctctccggc
     1261 agtttccgag atcttcgcga agttttcgac gaagaaggga agggtaacga agatcacgct
     1321 ctcttcgaga ttccccctgt acagagaatt ggcaaatact ggtttgatcg catcgaggta
     1381 atgctgtaga gagtagtaag tgtgcgcgag cgttccgttg atcgccgaaa ctatgaaggt
     1441 cttcaagcct ttctggtagt tttttagtat cctgagggca aggaaggggt tcttcaaacg
     1501 tatattgccg aaggcacccc agggactctt cgagcttaca tccatgaatt ccgggcattt
     1561 gtgcgagagc ttcttctcga gttcggaaat caaggccgag acgtcgttct tcacctggga
     1621 agccggttgt tgattcttcc agtcccttat gaattcgaag acgaaatttt tgtcccttac
     1681 ttcctcttcg aagacggggt ctatgtacag attgacatcg gaaagcgaga gcttcacact
     1741 ttgaagtccg taaaggttgt cactctttat cgaggcgacg gcgtccacct tcaaaagatt
     1801 gggttttacg tatttgaagt cgtcgaacat ggaactcatg ttgtagccta ttgccagcgt
     1861 ttttctatcg cccgatcgaa ggaccatctt cacgtgatcg cttccgttcc cgaaggtctt
     1921 ggccttttct atgttcaacc cttttatcag aaacttgggc tcgggattcg aatgtccaaa
     1981 cggtctgaga atctcgagct tgtccagtac gtggggagtt atcgaatcga gggacaatac
     2041 gtcgtccaca tctatcttaa agaccggtaa tttccttccg tattgcctta tataagcctc
     2101 gtttatagcc ttgcgaaact ctggaatgtt ttctttgcct atgctgaagc cggccgccat
     2161 ttcatgaccg ccgaactccc tgagcagaga ggagacttcg ttcaacaggc tgattatgct
     2221 gactcccgaa ggactcctgg ccgatccctt tccgtcctgt ccgttggtgg aaatgagaaa
     2281 gaccggtttg ttgtgtatag agacaagtct tgaagcaact atgcccagta ctcccagatg
     2341 ccagctgtca ccatcgatga cgagtgcgaa atcgttgctc attcgatggt ttatctccag
     2401 atctttggaa gtctgttcaa aaatcttggc ctcgatcgtc tggcggttct gattgtgttt
     2461 caggagtctg ctggccgtat tcatcgcaga atccatatcc tcgcttatca gtaactccaa
     2521 cgcgactatg gccgaatcca ttctaccggc tgcgttgagc ttgggtgcga ttttgaaggc
     2581 aatatcctgt gccatcaggt tttccgaagg aattctcaga tacgatagga gcgctttcaa
     2641 ccccagtaga gggctactct gtatcttcgc cgttccttct ctcactatgt acctgttctc
     2701 gtcgcatagc ggtacaatgt cggctatagt tccaagggcg actatgtcta gatagtcttc
     2761 gggattgata gggcagcgca acgtttcgtt caacgccacc agaagtttga aggcaactcc
     2821 tacaccggcc agtcccttga agggataaga gtcgtccggt ctcttgggat tgatcaccgc
     2881 gttcgcagga ggaaggatat ccttcacctc gtgatggtcg gtgacgacca cgtcgaatcc
     2941 tatctctcta gcgtactcta tttcaccgaa cgacgtaact ccacaatcga cagttatgag
     3001 gagactgtga cccct
//
LOCUS       DAOOLY010000282         3012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_39743_length_3012_cov_4.393304, whole genome shotgun sequence.
ACCESSION   DAOOLY010000282 DAOOLY010000000
VERSION     DAOOLY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39743_length_3012_cov_4.393304"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1779
                     /locus_tag="PLD15_11070"
     CDS             91..1779
                     /locus_tag="PLD15_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64496.1"
                     /translation="MRTGKAFLLTAILVLLVLTSCVKPSIPDESFTYGNTLLVSFVEY
                     VTGPVAAGVAVEVFDGESGELIGEGVTNESGQAFIELVWHLEEENADERTVTIVASKE
                     DMATSAMKGLKVPLHSLSEQIDTGPVMADMAIRRAMVDPESSRFPNLDIVLSGVTGEA
                     TDPTGLSFFKVKLDSDLEYESWFRLYVGVGYVPWEMGYDFMSANRSEMTFTVTSNGYS
                     GEVPIHVVVYDFNRARLDQVIYLNFAGGETTQVEKFAPSGLSVLSITSDTRFEYCSLS
                     EETVEETPTPDEAEYGEAISKEETNIYIKLSWKRPQDDVGLAGYNIYHSVDGVTYRKV
                     AFRKSPDFFDSGIGIEAGKRYYYKVRSLYLDGSESEDSNVVSLVPLDIFKVKLISPLN
                     NSTGVSRRPVLRWAPVDKKDHNKVPVLDGGLLNENEIVYHYTCMIWDTVQSGPQLIFP
                     LNQDLERFDFVTEGPRQVSVKFLDPSWSGRGMDWFFLLNTEMEMFPFDRLEAFKTYEW
                     GLDYAYACYTGDDSDGNGSPDSMVYSVTIDKSDVVDLRVNHADYLNRFTTWQDN"
     gene            1794..>3012
                     /locus_tag="PLD15_11075"
     CDS             1794..>3012
                     /locus_tag="PLD15_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPB64497.1"
                     /translation="MKMKRIKLFVVVAVLVSIFTSCVKFPSGDSIIADVETVWREFQS
                     APHLEGRLILGYSDYDVVEEICRLLGAEVTVDIKMLKAVGLRFDSTFEDALEQMKGLF
                     ESKPELLRAVRYIEPNYEMELIAPSLDEVVGVAGQTVPTAPGEIPNFEQFLWGIRKIR
                     APQVWDAGYDGTGIIVAILDTGIDSTHPDLQGQIVSRYDPLKGSIIDPEIDYSFGFHA
                     THVAGIIAARKNGSGVTGVAPGAKLMDIPIFQPEFIGDEFVAAGIVYAVDNGATVLSN
                     SWDGKGFSTLLYEAISYASRAGVVFVNSAGNDHVNEVRNPSVYPGVVMVASSSAIDGI
                     AYSSNRSVKLSVAAPGDSTILSTIPLWAEEYYSYPGLPYAYFGGTSMACPHVSGAIAI
                     LQQKYKDMGLTVYQ"
BASE COUNT          696 a          713 c          863 g          740 t
ORIGIN      
        1 tcatatatat tagtctctag attaagtatt tcggatttgt gatcgtataa ttgagaggat
       61 cgagagattt ccagtagtgg aggttttgtg atgaggacag gtaaagcgtt tctgttgacg
      121 gctatattag tgctgctggt tttgacttcc tgtgtgaagc cttcgatacc cgacgagtct
      181 ttcacttacg gcaacacgtt gctcgtgagt ttcgtggagt acgtgacggg tccggtggca
      241 gccggcgtgg ccgtcgaggt ctttgatggt gagagcggcg agcttatagg agagggtgtg
      301 actaatgagt caggtcaagc gtttatagag ctagtgtggc atctggaaga agagaacgca
      361 gacgagagga cggtcacgat cgttgccagc aaagaggaca tggccacaag cgccatgaag
      421 gggttgaagg tgccgcttca ttctctgtct gagcagatcg ataccggacc ggtaatggcc
      481 gatatggcca tccgcagggc gatggtcgat cccgaaagtt cgcgttttcc caatctggat
      541 atagttctat ctggcgttac gggagaggcg acggatccga ccggtctatc gtttttcaag
      601 gtaaaactgg actcggatct ggagtatgaa tcctggttcc gcctctatgt aggagtaggg
      661 tacgtaccct gggagatggg atacgatttt atgtcggcaa acaggtcgga aatgactttt
      721 accgtcacct cgaacggcta ttccggtgaa gtgcccattc atgtagtggt ttatgatttc
      781 aacagagcca ggctcgacca ggtgatctat ctaaatttcg ccggtggtga gaccacacag
      841 gtcgagaaat ttgcgccctc cggcctttct gtgctttcca ttacctccga cacgagattc
      901 gagtactgtt cgctttctga agagaccgtt gaagagactc ccaccccaga tgaagcggag
      961 tatggagagg ccatctcgaa agaagagaca aacatctata taaaactgtc ctggaaaaga
     1021 ccgcaagatg acgtgggcct cgcgggttac aacatctacc attccgttga cggtgtgact
     1081 tacagaaagg tcgccttcag gaaatctcca gatttctttg actccgggat aggtattgag
     1141 gccggtaaga gatactacta caaagtgagg tcactctacc ttgacgggag cgagtcggag
     1201 gacagcaacg tggtgtcgct cgtgccgctg gatatcttca aggttaagct tatatccccg
     1261 ttgaacaact ctacgggagt ttcgagacgg ccggtcttgc gctgggcgcc tgtagataag
     1321 aaagatcaca ataaggtgcc ggtacttgac ggggggttgc tgaacgaaaa cgagatcgtt
     1381 taccattaca catgcatgat atgggacacc gttcagagtg gtcctcagct gatcttcccg
     1441 cttaaccagg atttagagag gttcgatttc gttacagaag gaccccgaca ggtatcggtg
     1501 aagtttctcg atccctcctg gtctggcagg ggcatggact ggtttttcct gttgaacacg
     1561 gagatggaga tgttcccatt cgaccgtctg gaagccttca agacctacga atggggcctc
     1621 gactacgctt acgcctgtta tactggcgac gacagcgacg gaaacggttc gccggattct
     1681 atggtctatt ctgtgacgat cgacaagagc gatgtcgtgg atctacgcgt caatcacgcc
     1741 gattatctca accgtttcac gacctggcag gacaactgaa gggggtggtg taaatgaaaa
     1801 tgaaaagaat caagttgttt gtggtcgtcg ctgtgctggt ttcaatattc acctcctgtg
     1861 ttaaatttcc ttcgggggat tccataatcg cagatgtcga gaccgtctgg agagaatttc
     1921 agagcgcacc tcatctcgaa ggccggctca ttctggggta ttccgattac gatgtggtcg
     1981 aagagatctg caggttgctc ggggcggagg tcacggtcga tataaagatg ttgaaggctg
     2041 tcggtctgcg cttcgattcc actttcgagg atgccctgga gcaaatgaaa ggtctcttcg
     2101 aatcgaaacc cgaactattg agagcggtga gatatataga gcccaactac gagatggaac
     2161 ttatagcgcc ttcactggat gaagtggtcg gtgtggccgg tcagacagtc ccgaccgctc
     2221 cgggtgagat cccgaatttc gagcagttcc tctggggaat caggaagatt cgcgctccac
     2281 aggtctggga tgccggatac gatgggacag gtataatcgt cgccatcctc gatactggca
     2341 tcgactctac ccacccagat cttcagggcc agatcgtcag cagatacgac ccgttgaagg
     2401 gttcaataat agatccggag atagattact ctttcggttt tcatgctacg cacgtcgccg
     2461 gtatcatcgc ggccagaaaa aacggcagcg gtgtcaccgg tgtggcgccc ggagccaagt
     2521 tgatggatat accgatcttc cagcccgaat ttataggcga cgagttcgtc gcggccggca
     2581 tagtctatgc cgtcgataac ggggccacgg tactttcgaa ctcctgggac ggcaaggggt
     2641 tttcgacctt actttacgaa gcgataagtt acgcttcgcg ggccggggtg gtcttcgtga
     2701 actcggccgg aaacgatcac gtcaacgagg tccgcaaccc ttcggtttat ccgggagtgg
     2761 ttatggtggc ctcatcgtcg gccatcgacg ggatagctta ttcttcgaac cggagcgtca
     2821 aactctcggt ggccgctcca ggtgattcta cgatcctttc gaccattccg ctctgggccg
     2881 aggagtatta cagctatccg ggactcccct acgcttactt cggcgggacg tcgatggcct
     2941 gcccgcacgt ctcgggcgcg atcgccattc tccagcagaa atacaaggat atgggactga
     3001 cagtttacca gt
//
LOCUS       DAOOLY010000283         2998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_40008_length_2998_cov_3.855250, whole genome shotgun sequence.
ACCESSION   DAOOLY010000283 DAOOLY010000000
VERSION     DAOOLY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40008_length_2998_cov_3.855250"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1716)
                     /locus_tag="PLD15_11080"
     CDS             complement(148..1716)
                     /locus_tag="PLD15_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64498.1"
                     /translation="MSRRIFLAIIVVLLLIALSGCINILRKYQVTITSTPLLESVTVG
                     KRERELPYGTTAKRGEKILIKAPLVPGYDFAGWLVNNDLKEEANVEIIIEENTAIKAI
                     YIPKLVNEITLGGKGDEAGSGLIVESTSSLVAGVTSNSTDSNLIVASNGGFDFWVPPL
                     EYISNSFLVSPRKAHGGSGDEFLSDTTRITGGTVSVGWTDSQDIPFTGSGYPYHGGID
                     AYAIMLLNDGTLGWQTYIGGSGDDYAHSVNITVGERLILTGSTGSNDSNFEGTGHHGD
                     IDGWVSIMSSYGTLVSKLRKSFGGSGIDELFYGVQTRDGGYLLCGYSRSSDDDLKNID
                     RSQGEDLWILKVDEELNILWQDLLGGTADERAHVVKEVSDGLIVAGYTASTGRDVNDH
                     KGGKDIWVLKFKDDGELLWSKTYGGTDDEEAFDIIETADGGYAVCGYTKSTNRDVRAN
                     KGMSDIWVIKLGTDGYLEWEATFGGTQNDGGRRIGETEKRELIVIGTTSSNDRDVLSG
                     LHGGTDAWLLRLGK"
     gene            1875..2078
                     /locus_tag="PLD15_11085"
     CDS             1875..2078
                     /locus_tag="PLD15_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64499.1"
                     /translation="MESDSCSNYHECPIVKAYEQGKIDSEWINMYCHDRWKDCERFQL
                     EDLSDSSYEFVLPDGSVEERLRR"
     gene            complement(2194..>2998)
                     /locus_tag="PLD15_11090"
     CDS             complement(2194..>2998)
                     /locus_tag="PLD15_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64500.1"
                     /translation="NDTSLAMAIKEYGSPEQVAARYYEDGPIIAPHLKNYLFMYSGII
                     FAIHIGLHLLSLIFGDNGAIFKFSSTDLLGVLSQLPVTFVFDFGLVSLILYFVTKAGA
                     TSSLPYFTWFLKSEKIPTQAQRIASLVASIIGAVITYLGFTYGPVYFVDGQMNTLAIA
                     ALDTMKYGLFLGFGILVISSIFNLINVFNYSRIVKIASDIAGLIFLLLVVSPDYSGRI
                     ADFLSISPESTGHPLLIGILVFIALVTIIELVIETIRFWASRIVTSTNM"
BASE COUNT          720 a          775 c          747 g          756 t
ORIGIN      
        1 agcagattgt agaaggcgcc gtaaatccag ccgtccacgg ctttggaacg ggccgagcag
       61 acggggcaga tgtcggctct ggcaagaagt tcgacgactt cgctttcaac tacggtctct
      121 actctcaact ccggtccctt cggccgctca tttgcccaga cgcagaagcc aggcgtctgt
      181 cccaccgtgg aggccgctga gaacgtctct gtcgttggag gaggtcgtac cgatcactat
      241 caactctctc ttttccgtct ctccgattct cctgccgccg tcgttctgag tacctccgaa
      301 agtggcttcc cattccaggt agccgtcagt tccgagctta atcacccaga tgtcgctcat
      361 acctttgtta gccctcacgt cacggttggt agattttgta tagccgcaca cggcgtagcc
      421 tccgtcggca gtctctatga tatcgaaggc ctcttcatcg tccgtgccgc cgtacgtttt
      481 gctccacaat agttctccat cgtctttgaa tttcaagacc cagatgtcct ttcccccctt
      541 atggtcattt acatcccttc cggtggaggc cgtgtaaccg gctacgatca aaccgtcgct
      601 aacctcttta accacatggg ctctctcgtc tgcggtacca ccgaggaggt cctgccagag
      661 aatgttgagt tcctcatcga ccttgagaat ccagagatcc tcgccctgag accggtcgat
      721 gttcttcaga tcatcgtcgc tggacctgga atagccgcag agaaggtaac cgccgtcgcg
      781 tgtctggaca ccgtagaaga gctcatctat accactgccg ccgaaggatt tcctcaactt
      841 cgatacgagg gttccgtaag agctcattat cgatacccag ccgtctatgt cgccgtggtg
      901 gccggtaccc tcgaagttag aatcgttcga gccggtcgat ccggtaagga tcaaacgttc
      961 accgacagtt atgtttaccg aatgggcgta atcgtcgccg cttccaccta tgtacgtctg
     1021 ccagccgagt gtgccgtcgt tcagcagcat gatcgcgtag gcgtctattc caccgtgata
     1081 tggatagccg ctacctgtga aaggtatatc ttgcgaatct gtccagccaa ccgaaacggt
     1141 accaccggtt atccgggtcg tgtctgagag aaactcatcg cccgaaccgc cgtgagcctt
     1201 tcggggggag accagaaagc tgtttgaaat atattccagt ggaggaaccc agaagtcgaa
     1261 tccgccgttg ctggcgacta taaggttact gtcggtcgag ttggatgtaa cgccggccac
     1321 caggctcgaa gtgctctcca ctatcaggcc ggaaccggct tcgtcgccct tcccgcccag
     1381 agtgatttca ttcactagct tcggaatgta gatagctttt atagccgtgt tctcttcgat
     1441 tattatctca acgttggcct cttccttgag gtcgttgttc acgagccatc cggcaaagtc
     1501 gtatccgggg acgagaggag ccttgataag gatcttctcg cctctcttcg cggtcgtccc
     1561 atagggaagc tctctctccc tctttccaac ggtcacactc tcaagcagag gtgtgctggt
     1621 gatagtcacc tgatatttcc tcaagatatt gatacagccc gaaagcgcta tcagtaaaag
     1681 aacaacaatt atcgccagaa aaatccttct ggacatccaa tcacccccaa gttattgtct
     1741 cccattcgtt atattttata acgagagaag gccattgtat aaggaaatcg acaggcccga
     1801 aggtaaagat acttgttttt cccgccccat gtgataacat tagtaatgaa atatccagtg
     1861 gaggtgtccc gctagtggaa agcgacagtt gcagtaatta tcatgagtgt cccatagtga
     1921 aggcttacga acagggaaag atagacagcg aatggatcaa tatgtattgc cacgatagat
     1981 ggaaagattg cgaaagattt cagctcgaag acctcagcga ttcctcttac gaattcgttc
     2041 ttcccgacgg aagcgtcgaa gagagattga gacggtgatc cccgtcaaac cagttattgc
     2101 ttaatagcgt ctcttgctcc taatttcctc tcgagagaga atcaggagca agagttcgaa
     2161 aacatcaaat taaggttata gacaataggg ttcttacatg ttggtggatg tcactattct
     2221 gctcgcccag aaccttatgg tctcgatgac aagctctatt atggtcacta aagctatgaa
     2281 aacgagtatt ccgatcagga gcgggtggcc ggtgctttcc ggagatatgc tgaggaaatc
     2341 ggctattctt ccggagtaat cgggtgagac tacgagtaga agaaaaatca gcccggcgat
     2401 atcggaggct atcttcacta ttctggagta gttgaacacg tttatcaggt tgaagatcga
     2461 cgagataacc agtattccaa agccgaggaa gaggccgtat ttcatagtgt caagcgcagc
     2521 tatggccagc gtgttcatct gaccgtcgac gaaataaacc ggtccgtaag tgaagcccag
     2581 gtatgtgatc accgctccta ttatcgatgc cacaaggctg gctattctct gggcttgagt
     2641 gggaattttc tcgctcttca gaaaccatgt gaagtacggg aggctcgatg tagcgcccgc
     2701 tttagtgacg aaataaagga taagactgac cagaccgaaa tcgaagacaa aggttactgg
     2761 aagctgtgac aggacgccca gcaaatccgt actgctgaat ttgaagatcg ctccgttatc
     2821 tccgaagatc aggctcagca gatggagccc gatgtggatc gcgaagatta ttccgctgta
     2881 catgaataga taatttttaa gatgaggggc tattataggg ccatcttcgt agtacctggc
     2941 agcgacctgc tcgggtgaac cgtactcttt tatcgccatt gcgaggctgg tatcgttt
//
LOCUS       DAOOLY010000284         2993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_40108_length_2993_cov_3.356705, whole genome shotgun sequence.
ACCESSION   DAOOLY010000284 DAOOLY010000000
VERSION     DAOOLY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40108_length_2993_cov_3.356705"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLD15_11095"
     CDS             complement(<1..246)
                     /locus_tag="PLD15_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB64501.1"
                     /translation="MNIDEVIQRNISSGRRLKLLALTSLEWMLVAGGVMVTSLTLPSI
                     IEGLSGTVGDSTTMASSVFIGMLVGALVSGAISDRFGR"
     gene            complement(243..767)
                     /locus_tag="PLD15_11100"
     CDS             complement(243..767)
                     /locus_tag="PLD15_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HPB64502.1"
                     /translation="MNKQARLLKITKLLQDSNGAISGSELARLTGVSRQMIVKDIEEL
                     RYQGYRIQSGQKGYSLSTQKKCRMTLSVKHGDSDIVDELSTILSEGGAVIDVVIMHPV
                     YGEIRGVLNLHDFDDLNRFIASLRSSEAVPLLALSKDGVHLHTIEADSEDGLEKIRAA
                     LEKKGYLAVRGVGL"
     assembly_gap    924..999
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1205..1861
                     /gene="minC"
                     /locus_tag="PLD15_11105"
     CDS             1205..1861
                     /gene="minC"
                     /locus_tag="PLD15_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPB64503.1"
                     /translation="MPIDFRMTKKGLILLIDSYRTVEELRQDIMRKFTDAKDFFSEGD
                     EISLMLTQETSKPDDIVKIVSLLSDLGVHVKDILVGSMEQKDVKVGQKYDLVREKITE
                     VRGAQIIRRNLRSGQIVVHNYDVVIVGNVHPGAEVISGGSIVVFGTARGVLRAGYSQG
                     EEGIIAAIDLSPSLLQIGNYITQEYDRFDVPSVAHVRTGRIVVEEADNVKFEVKGGTN
                     "
     gene            1858..2871
                     /locus_tag="PLD15_11110"
     CDS             1858..2871
                     /locus_tag="PLD15_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPB64504.1"
                     /translation="MNTARLLIELSNAFGPVGYEDRVHEVIKAEVGPYVDEIYRDEIG
                     NLIAVKKGNSRRVGVFTHIDEVSLVISAIDERGFARFESLGGVDPKILISQKVRIECR
                     DGKERKGVIGMLAPHLQKPGSRGDVPSFDELFIDASINQDFDRIDIGDLAVVDFTAFE
                     MGEKVSGKALDDRACAAISMETAKLLSMYREHPTVYFIFTTREEVGAMGAKAAAEALK
                     LDLGLAMDVTHHSKEDGIEIAKGPVITVGGPNIHKGYFKKLDDYARENGFPVQYDYGP
                     GHTGTDADVVQLAGNGTPTLLLSLPQLFMHTPVEVIAVCDVLNSARLLSGFFSSLEEV
                     EVV"
BASE COUNT          759 a          703 c          761 g          694 t
ORIGIN      
        1 tcttccaaac ctgtcggata tcgcccccga tacgagcgcg cccaccagca taccgataaa
       61 aacggaactt gccatggtcg tcgagtctcc cacggtcccc gaaagtcctt cgataatcga
      121 gggaagcgtt agcgaggtga ccataacccc tcccgcgacc agcatccatt cgagtgaagt
      181 taaagcgagc agcttcaatc tccttccaga agaaatgttt ctctgtataa cctcgtcgat
      241 gttcataaac ccaccccccg gacagccaga tatcctttct tttcgagtgc tgccctgatt
      301 ttttcaagac catcttcgga atccgcctca atcgtgtgta gatgcacgcc gtcctttgaa
      361 agcgccagga gaggaacggc ctcgcttgac ctgagagagg cgatgaacct gttcaggtcg
      421 tcgaagtcgt gcagattcaa cactccccga atctccccat agaccgggtg cattatcact
      481 acgtctatca cagctccgcc ttcggatagg atcgtggaga gttcgtccac gatatccgaa
      541 tctccgtgtt ttacagacag agtcattctg cacttttttt gcgtggatag agagtaacct
      601 ttctggccgg attgtatcct gtaaccctgg tagcgcagtt cttcaatatc tttgacgatc
      661 atttgtctgc tgacgccggt cagcctcgcc aactcgcttc cgctgatcgc gccgttcgag
      721 tcctggagaa gcttcgttat cttcagcaac ctggcctgtt tgttcaccat ctaccctcct
      781 atttacaggt tatagcagct actttacact acattttaaa agaagaaacg ccccttgtcg
      841 agggggcgtt gtaacaacta gctttaactc tggtggcaag ggacggaatc gaaccgtcga
      901 cacacggatt ttcagtccgc tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ccccggtcgc cggcgtgaaa
     1021 ggccagtgtc ctaggccact agacgatgag ggcgtaagca cgtaatttat attacactat
     1081 aatgcttctc cggtcaaggc cctgaacgga attatccacg ctgatatcta gcccatcgac
     1141 cggaatcggc tgaactttca gagttgtttt tcgtgataaa atcttgaaga aaggagtgat
     1201 ctggatgcca atagacttca gaatgacgaa aaaagggttg attcttctta tcgattctta
     1261 cagaaccgtc gaagaattgc gacaggacat aatgagaaag ttcaccgatg cgaaggattt
     1321 cttttccgaa ggagacgaga tatctctgat gctcacgcag gaaaccagca agccggacga
     1381 tattgtcaag atcgtgtcgt tgttgagcga tctcggagtc cacgtgaagg atattctcgt
     1441 cggaagtatg gaacagaaag acgtcaaagt cggtcagaaa tacgatctgg taagggaaaa
     1501 gatcacggaa gtcagaggcg cacagatcat ccggaggaat ttgaggtccg gccagattgt
     1561 tgttcacaat tacgatgtgg ttatcgttgg caacgttcat ccaggtgcag aggtgatctc
     1621 gggaggaagt atagtggtct ttggaactgc ccggggagtc ttgagggccg gatattccca
     1681 gggtgaagaa ggtatcattg ccgccatcga tctctcacct tctcttttgc agataggaaa
     1741 ctatataaca caggaatacg accgcttcga tgttccatcg gttgctcacg tgagaacggg
     1801 tagaatcgtc gtcgaagagg cagacaacgt caagttcgaa gtaaaggggg gaacaaattg
     1861 aacacagctc gactattgat cgaactttcc aacgctttcg gacctgtagg ttatgaagac
     1921 agagttcacg aagtgatcaa agccgaagtg ggtccatacg tggacgagat ctacagagac
     1981 gagataggca accttatcgc cgttaaaaag ggtaactcca gacgggtggg agtcttcaca
     2041 cacatagatg aagtctctct ggtgatatcg gccatagatg aaagggggtt cgccagattc
     2101 gagtcgctcg gaggggtcga tccgaagata ttgatttccc agaaagtcag gattgagtgt
     2161 cgtgacggga aggaaaggaa gggcgttatc ggaatgctcg ccccgcatct ccagaaaccc
     2221 ggttcgaggg gagacgtgcc ttccttcgac gagctcttca tagacgcctc aatcaatcag
     2281 gacttcgaca gaatcgatat aggcgacctc gcggtcgtag acttcacggc cttcgaaatg
     2341 ggcgaaaagg tttcgggaaa agctctagat gatagagcgt gcgccgcgat aagcatggag
     2401 acggctaaac ttctatctat gtaccgtgaa caccccacag tttattttat cttcaccaca
     2461 cgcgaagagg tcggggcgat gggcgccaaa gcggctgccg aagctcttaa gcttgatctg
     2521 ggcctagcca tggacgttac gcatcacagc aaagaagatg gaatcgaaat agcaaagggg
     2581 cctgtgataa ctgttggagg gccgaacatt cacaaaggat acttcaagaa attagacgac
     2641 tacgcaaggg aaaacggatt cccggttcag tacgattacg gtcccggaca taccggcaca
     2701 gatgccgacg ttgtccagct tgctggaaac ggaactccaa ctttgctact ttcccttccc
     2761 cagctcttca tgcatactcc ggtagaggtg attgcggtgt gcgacgttct caattcggcc
     2821 agactgcttt ccggattttt cagttcactc gaggaggttg aggtagtatg aagctcgaaa
     2881 gactgtgtaa gctttgtgaa ctccccggga tttcgggatt tgaagagaaa gtggcctctt
     2941 tcatcgagaa agagatagag ggaaaggtcg atgagctctg gaaagattcg gtg
//
LOCUS       DAOOLY010000285         2964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_40648_length_2964_cov_4.944311, whole genome shotgun sequence.
ACCESSION   DAOOLY010000285 DAOOLY010000000
VERSION     DAOOLY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40648_length_2964_cov_4.944311"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLD15_11115"
     CDS             complement(<1..720)
                     /locus_tag="PLD15_11115"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPB64505.1"
                     /translation="MNQALKPVLCAGEILYDFISRDRMQGLGGTTLFEKRPGGAPFNI
                     AAGLQKLGVPTAFLSKIGFDEFGEALVKYLNELGIRTNYIVRETGTKTTLAFAALDSD
                     GKPEFRFYRDRAADISLRKDELKDLSPEDFSLFHFGSIALLDEPSASTYIDLFERFKK
                     AGVRTSFDPNVRKNAVVDHDTFRRKLREIMRRVDILKLSDEDLEYIAGKGDVEKAVES
                     LEVGRDELVLVTLGSKGARAFW"
     gene            complement(717..1364)
                     /locus_tag="PLD15_11120"
     CDS             complement(717..1364)
                     /locus_tag="PLD15_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPB64506.1"
                     /translation="MIYIDGICERAVQLVKEPVFAGLTTNPTIIKRDRPGWGMMDALK
                     FLSKVPGERHFVQGSILSGNWVQKVREFLKKSDFDPELFTIKLPWDPQKASAIVPELN
                     EMGVGVCATAVYTVQQYYAALSMEVEYVAVYYDRMIKAGIDAEERIEEMLEVGGWHSS
                     APHIIAASIKDIESANLLLALGVHDLTLPVDIAGEYIMGSFPADDLNRFEGDFKL"
     gene            complement(1388..2806)
                     /locus_tag="PLD15_11125"
     CDS             complement(1388..2806)
                     /locus_tag="PLD15_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HPB64507.1"
                     /translation="MQKRKVGVITFSDGRDFVHRDLIDMNREFQNRLVRALEATGEVE
                     VVVASEIVWKPSLAKKAGKEMMKAEVEATIFNYAIWCWPHLTVLASLYAPGPYLAYGQ
                     INPAYPGMVGLLAAAGALEQVGITPERVWGNPEDPDVIEKVMHFVRAASAAARLKGER
                     YGMFGGRPMGMYTASSNGDQWLKEFGIDVEQIDQYELVVRAEKVSKEVTKKAREWLEK
                     LATVQYDGKQLTPEKLEKQIALYEAALDIIKEYELDFVGFKGQPEMTNNYATMDVVEA
                     FLNDPYDWHGMKDPIVAATETDMDGALTMEIFKHIAQAPVLFADVRHYFEEENLLDLC
                     NSGTHATYFAGRSLNPEENLKNVVFYPEDFYFPAGGAAVKHFAAPGRVTLARLARQDG
                     EYVMTIVPGEFVKLSAAEEKKLSEEVQIQWPHAYVRLDTDMDTFLKYYPCNHIHGVYG
                     DYVEELVQFCDIKGIDYQILGE"
BASE COUNT          589 a          874 c          733 g          768 t
ORIGIN      
        1 ccagaaggcc ctggctcctt tacttccgag cgtgaccagc accagttcgt cgcggccgac
       61 ctcaaggctt tcgaccgcct tttcaacgtc gccttttccc gctatgtatt ccaggtcctc
      121 gtcgctcagc ttcagaatat ccacccggcg cattatctcc cttaactttc ttctgaaggt
      181 atcgtgatcg acgaccgcgt tttttctcac gtttggatcg aaagaagtcc tcactccggc
      241 cttcttgaac ctctcgaaaa gatcgatata ggtagaagcc gacggctcat ccagaagagc
      301 gatggagccg aagtgaaaaa gcgaaaaatc ttcgggtgag agatccttca actcgtcttt
      361 gcggagcgat atgtcggcgg cgcggtctct gtagaacctg aactcgggct ttccgtcgct
      421 gtcaagtgcc gcgaaggcca gcgtcgtttt tgtgccggtc tctctgacga tgtaattggt
      481 cctgatcccg agctcattaa gatacttgac tagagcctct ccgaattcat cgaaaccgat
      541 tttcgaaaga aaggccgttg gaactccgag cttctgaaga cctgccgcta tattgaaagg
      601 cgcaccaccc ggtctctttt cgaacagggt ggtgcctccc agtccctgca tgcggtctct
      661 cgaaatgaag tcgtagagta tctctcccgc acagagaacc ggtttcaacg cctggttcat
      721 agcttgaaat ctccctcgaa tctgttcaaa tcatcggccg ggaagctccc catgatatat
      781 tctccggcta tatcgaccgg aagagtgagg tcgtgaacac ccagcgccag tagaaggttt
      841 gcgctctcta tatcctttat actggccgca attatatgcg gggcgctgga gtgccatcct
      901 ccgacttcga gcatctcttc gatcctttcc tcggcgtcta tgccggcctt tatcattcta
      961 tcgtaataaa cggcgacgta ctctacctcc atgctcagcg cggcgtagta ctgctgaacg
     1021 gtgtaaacgg cggtcgcaca gacgccaaca cccatctcgt tcaactcggg gacgatggcc
     1081 gaagcctttt gaggatccca gggaagcttt atggtgaaga gttcgggatc gaagtcgctc
     1141 ttcttgagaa actccctgac tttctggacc cagttgccgg agaggatact cccctgtacg
     1201 aagtgcctct cgccgggaac ttttgacagg aacttcaggg cgtccatcat gccccagccc
     1261 ggtctatctc gttttattat cgtcgggttg gtggtcaacc cggcaaagac cggttccttg
     1321 accagttgca ccgctctctc gcatattccg tcgatgtata tcatctcagt gtctcctccc
     1381 tttcaattta ctctccgagt atctggtaat cgattccctt tatatcacaa aactgtacga
     1441 gttcttcaac ataatctccg tacaccccgt ggatgtggtt gcaggggtag tatttgagga
     1501 aagtgtccat atccgtatcg agcctgacgt aagcgtgagg ccactggatc tgaacttcct
     1561 ccgagagttt cttctcctcg gcggccgaaa gcttgacaaa ctctccgggt acgatggtca
     1621 tcacatactc tccgtcctgc ctggccagcc ttgccagagt caccctgccc ggtgcggcga
     1681 aatgtttgac ggcagcccct ccggcgggga agtagaagtc ttcgggatag aacaccacgt
     1741 tcttgagatt ctcttcgggg ttgagagatc tacccgcgaa gtaggtcgcg tgggtgccgg
     1801 aattacagag atccaggaga ttctcctctt cgaagtagtg ccgaacgtcc gcgaagagga
     1861 ccggtgcctg tgcgatatgt ttgaatatct ccatcgtgag ggctccgtcc atatcggtct
     1921 cggtcgccgc gacgatcggg tccttcatgc cgtgccagtc gtaaggatcg ttaaggaagg
     1981 cctcgacaac gtccatggtc gcgtagttgt tggtcatctc aggctggcct ttgaagccga
     2041 cgaagtccag ctcgtactct tttattatat ctagagccgc ctcgtacagc gcgatctgct
     2101 tctcaagctt ttccggcgtc agctgcttgc cgtcgtattg gacagtcgcg agtttctcga
     2161 gccactccct cgcttttttc gtgacctctt tcgatacctt ttcggccctc actacgagtt
     2221 cgtactgatc gatctgttcg acgtcgatcc cgaactcttt gagccactgg tctccgttgg
     2281 aggaggcggt gtacataccc atagggcgac cgccgaacat cccgtacctc tcgcctttca
     2341 gcctcgccgc ggccgatgcc gccctgacga agtgcatcac tttctcgata acatccggat
     2401 cctccgggtt tccccagact ctttcgggag ttattcctac ctgctccaga gctccggcgg
     2461 cggccagaag accgaccatg ccggggtagg ccggatttat ctgaccgtac gccagatacg
     2521 gaccgggggc gtacagtgag gccagcaccg tcagatgggg ccagcaccag atggcgtagt
     2581 tgaatatagt cgcctcgact tcggccttca tcatctcttt tccggccttc ttggccagcg
     2641 agggcttcca gacgatctcc gaagccacta caacctctac ctcgcccgtg gcttcgagcg
     2701 ctctcaccag ccggttctgg aactctctgt tcatgtcgat gagatctctg tgcacgaaat
     2761 ctctaccgtc ggaaaacgtt ataactccta cttttctctt ctgcatgctc ctcctccttc
     2821 tatgccttaa acaatggcga ttgtcatagc cgttttacta tttttgccgt agaggctacg
     2881 gaatttcttt ccagcagctc ggccttcaac acgatcctgg tctgttcccc acggtccccg
     2941 tctatactcc cgagaagtag tgtg
//
LOCUS       DAOOLY010000286         2958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_40764_length_2958_cov_3.533930, whole genome shotgun sequence.
ACCESSION   DAOOLY010000286 DAOOLY010000000
VERSION     DAOOLY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40764_length_2958_cov_3.533930"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLD15_11130"
     CDS             <1..451
                     /locus_tag="PLD15_11130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB64508.1"
                     /translation="EAVTPFCRGKRGLDYGSGPSPVLATILERNYGFSMDIYDLFFSP
                     VMIYEGKVYDVITSTEVIEHLENPLEHFEIFKNSMGEGSVLSIMTLFHPESEEGFLNW
                     YYMRDMSHISFYTPQTMRVIGEKTNLEVIYTDNLRYTTYVKKRNTTG"
     gene            complement(539..1027)
                     /locus_tag="PLD15_11135"
     CDS             complement(539..1027)
                     /locus_tag="PLD15_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPB64509.1"
                     /translation="MTGTAVYYSFTGSTEVIAKSFSEKSGFKLCKLEDREKRKSAPAS
                     ASILGLGTALKEPLPDIKNVDFLVLLTPIYAWHPSAQMNTFLKNAGIGGKKLFLVGVG
                     AGETNEKAMARFSGKVKKLGGQVAGTKAFKGVQLGKNYKEVEAALQKSGEELAKIIDS
                     LT"
     gene            complement(1002..2159)
                     /gene="iadA"
                     /locus_tag="PLD15_11140"
     CDS             complement(1002..2159)
                     /gene="iadA"
                     /locus_tag="PLD15_11140"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HPB64510.1"
                     /translation="MLLLIKNINLYSPGPIGNMDMLIGGEEILKISENIPSSDILSMG
                     GRLVDGTGMIAVPGFVDGHTHLIGGGGEGGFSTRTPEGFAGQFIDVGTTTVVGMLGTD
                     GVTRGHASLLAKVRDFNNKGLNAFMLTGSYRYPLKTLTGDVMKDIVLVPEIIGVGEVA
                     ASDHRSSNITATELQRMAMDARVAGMLSGKSGVTVLHMGDALAGLKPLLEATDDGMLN
                     PKQIIPTHIDRTEKLLAEGLNWVKERDGYIDFTADENRTAHILADLHSRGYNFERLCV
                     SSDGLGSMPLFDAQKNLIGIRSGPVDTLRKTFVSLVKEHGLEIQRALMPFTSNPSRFY
                     KFDSSGLGFIKEGKKASFLILNDELEIKGVFSKGRFLKEGGSLDDRNGCVL"
     gene            complement(2185..>2958)
                     /locus_tag="PLD15_11145"
     CDS             complement(2185..>2958)
                     /locus_tag="PLD15_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64511.1"
                     /translation="DCTELINYIGNIRKREKELRLFFGLMGKREASIREEAIKMAREY
                     LPSSASFEKLNVYFVPMPYNANADNLGVYFDPIFAMDIGLDAITGVMAHEAHHIGRNS
                     VTSERLEFGDKPLETLAYRFMCLETEGIANLVNDASQIPSLKRIALTRTKIMAEFEKH
                     LELLQEVFLNLAGGVITDREARALMSRSWFTTGSLAPIGMKMAIEIENEFGKENLVKT
                     VGDTVAFLKAYQKVALKKNYYLLEDETFDNLEKLLKARQ"
BASE COUNT          604 a          762 c          812 g          780 t
ORIGIN      
        1 tgaagcggtg actcctttct gcaggggaaa gagaggtctt gattacggca gcggcccgtc
       61 tcccgtgctg gccacaattc tggagaggaa ctacggcttt tcgatggaca tttatgatct
      121 cttcttctcg cctgtcatga tttacgaggg gaaggtctac gacgtgataa cctcgacgga
      181 agtgatcgaa catctggaga atccgctcga gcatttcgaa atcttcaaaa atagtatggg
      241 ggagggaagt gttctatcga taatgactct ctttcacccc gaaagcgagg aggggttcct
      301 gaactggtac tatatgaggg atatgagcca tatctctttc tacactccgc aaacgatgag
      361 ggtcattgga gaaaagacca atctggaggt tatctataca gataatctgc gctacactac
      421 gtatgtaaag aaaagaaaca ccaccggttg agtcccagac ccgaccggtg atttgtttcc
      481 ccgacactct tgcgcttatc gctctagcgt tttatcacat tcgttgctta ccggcgcgtc
      541 aggtgaggct gtcgattatt tttgccagtt cttcgcccga tttctgcagc gccgcttcca
      601 cttctttgta gttctttccg agctgtacac ccttgaaggc cttggttccg gccacctggc
      661 cgcccagttt tttcaccttg ccggagaagc gtgccatggc tttttcgttg gtttcaccgg
      721 ctccaacgcc cacgagaaag agttttttgc caccgatacc ggcatttttt aagaacgtgt
      781 tcatctgggc cgaggggtgc caggcgtaga tgggcgtcag caggaccagg aaatcgacgt
      841 tttttatgtc cggcagaggc tctttgagag ccgtccccag accgagtatc gaggcggaag
      901 cgggagcgga ttttcttttt tctctgtctt ccagtttgca gagtttgaag cccgactttt
      961 cggaaaagct ctttgcgata acttccgtcg atccggtgaa actataatac acagccgttc
     1021 cggtcatcta atgaacctcc ctctttcagg aatcttccct tcgagaagac cccctttatt
     1081 tcaagttcgt cgttcaggat caggaaactg gcctttttgc cttcctttat gaatcccaga
     1141 ccggacgagt cgaatttgta gaaacgcgag gggttggatg tgaagggcat gagagcacgt
     1201 tggatttcga gtccgtgttc tttcacgaga ctcacaaagg tttttctgag cgtatcgaca
     1261 ggaccggatc ttatgccgat cagattcttc tgcgcgtcga agaggggcat cgagccgagt
     1321 ccgtcggagg agacgcacaa cctttcgaag ttgtagccgc gagagtgtag atcggccagg
     1381 atatgggcgg tacgattttc gtcggcggtg aagtctatat agccgtcacg ctccttcacc
     1441 cagttaaggc cctcggccag gagtttttct gtgcggtcta tgtgggtcgg tatgatctgt
     1501 ttaggattca gcatcccgtc gtcggtggcc tccaggagag gtttcaaacc ggccagggcg
     1561 tcgcccatat ggagaaccgt caccccggat ttgcccgaaa gcataccggc aactctggcg
     1621 tccatagcca ttcgctggag ctctgtagcg gtgatgttac tgcttctgtg atcggaagcg
     1681 gccacctctc ccaccccgat tatctcgggc accagaacta tatccttcat cacgtcgccg
     1741 gtgagagttt tgagcgggta cctgtaggag cccgtgagca tgaaggcgtt gagtcctttg
     1801 ttgttgaagt cgcggacttt ggccagtaac gaggcgtggc ccctggtgac gccgtcggtg
     1861 ccgagcatac cgaccaccgt ggtcgttccg acatctatga actgcccggc gaagccctcg
     1921 ggagttctgg tggagaagcc gccctcgccg ccgcccccta tgaggtgtgt gtggccgtcg
     1981 acgaaacccg gtacggctat catgccggtc ccgtcgacga gtcttccgcc cattgaaagt
     2041 atgtcgctcg agggtatgtt ttcggagatc ttgagtatct cttcgccgcc gatgagcata
     2101 tccatgtttc cgatcggccc gggcgagtaa aggtttatat tttttatgag caaaagcatc
     2161 ggatgcacct ccggcgatag atagttactg acgggccttg agaagctttt ccagattgtc
     2221 gaaagtctcg tcttcgagaa ggtagtagtt cttcttcaga gccactttct ggtaagcttt
     2281 caggaaggcc acggtgtcgc cgacggtttt cacgaggttt tcctttccga actcgttctc
     2341 tatttcgatg gccattttca tgccgatggg cgcgagactg cccgtggtga accagctgcg
     2401 ggacatgaga gcgcgggcct ccctgtcggt gatgactccc ccggcgaggt tcaaaaagac
     2461 ctcctggagg agctcgagat gtttctcgaa ctcggccatg attttggttc tggtaagggc
     2521 tatccttttc aaggagggga tttgcgaagc gtcgttgacg agattggcga tcccctccgt
     2581 ctcgagacac atgaacctgt aagccagcgt ttcgagaggt ttgtcaccga attccaggcg
     2641 ctcggaagtg acggagtttc tgcctatgtg gtgcgcttcg tgggccatta ccccggtgat
     2701 ggcgtccaga cctatgtcca tggcgaagat cggatcgaaa tagacgccca ggttatcggc
     2761 gttggcgttg tagggcatcg gcacgaagta aacgttcagc ttctcgaagg aggccgaaga
     2821 cggcaggtat tcccgtgcca tttttatcgc ttcttctctt atagaggcct ccctcttgcc
     2881 catcaggccg aagaagagcc tcaactcctt ttcacgcttt cttatatttc ctatgtagtt
     2941 tataagttcg gtgcagtc
//
LOCUS       DAOOLY010000287         2930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_41314_length_2930_cov_5.014957, whole genome shotgun sequence.
ACCESSION   DAOOLY010000287 DAOOLY010000000
VERSION     DAOOLY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41314_length_2930_cov_5.014957"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLD15_11150"
     CDS             <1..313
                     /locus_tag="PLD15_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64512.1"
                     /translation="APKNFILGQGLYGDVVYDMMGNITGFEGMDFFLFQASTYYEKPL
                     TPNEVNAFTFDGKFLFFIPVFGRGWEFLLRPAESLILSAGLSLLYNFLPGVYLNLTIL
                     L"
     gene            482..1723
                     /locus_tag="PLD15_11155"
     CDS             482..1723
                     /locus_tag="PLD15_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPB64513.1"
                     /translation="MNTIKEDARYIIERSIETVLPEKAVFNSLESLHLPGKTFLLAIG
                     KAAWRMAKAAREKLGQSITAGVIITKYGHVEGDIPGIDSFEAGHPLPDENTIAATTKA
                     LEMINAINPDSILFLISGGGSALFEKPVEGVTLDDLVDINNRLLRSGANIVEINAVRK
                     RLSVVKGGRFAKMFAQTTIHSLVLSDVLGDRLDSIASGPAYPDSSTCEDALSTIDRYG
                     LVLRPELREILKIETPHLLSNVQTRIIGSVGRACEAAGVFAKELGYEPMILTTTLDCE
                     ARDAGSFLAAIAREEVYDRPLKRPCAIILGGETVVPVRGTGLGGRNQELALSFAIASN
                     GLDNVALASVGTDGTDGPTDAAGAIVDGGSAREMIERGIDPHIYLLDNDSYHALEMSG
                     ELLKTGPTGTNVNDLVVLLCR"
     gene            complement(1780..2346)
                     /gene="pth"
                     /locus_tag="PLD15_11160"
     CDS             complement(1780..2346)
                     /gene="pth"
                     /locus_tag="PLD15_11160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPB64514.1"
                     /translation="MFLFIGLGNPGPRYVFTRHNVGFLFVDEMMRNGVRKTSKKKIYE
                     AYLLEGEPKIYLAKPLTFMNLSGIAVKSLIADLNLTREDTVVIVYDDIWIPLGRIRIR
                     ENGSDGGHNGLKSIITELGTKDFPRIRIGVGPLPENRDMIDYVLGQFSDEDYRTVSKA
                     IDLAVQSAREIVAGDFKKVMSKYNGMEV"
     gene            complement(2350..>2930)
                     /locus_tag="PLD15_11165"
     CDS             complement(2350..>2930)
                     /locus_tag="PLD15_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPB64515.1"
                     /translation="NQGKIPAVVYGPEVETFSVSLDKVEVLRNINKIAETTSVILSLD
                     GKEHHVFLKSVQRNKVTDAIIHLDFYSPSKGRRMEIHIPIRLSGKPIGIEKGGIVEHI
                     INELPVSVLPKDIVESIDVDISSFDVGKILRVKDLPIPEGMKPLADGEEPVLVIELPR
                     AARGAEVEEAPEEKEGTEPEVINKGKKEEEEE"
BASE COUNT          695 a          727 c          750 g          758 t
ORIGIN      
        1 agctccaaag aacttcattc tcggtcaggg tctctacgga gatgtggtgt acgatatgat
       61 gggcaacatc accggtttcg agggaatgga cttcttcctt ttccaggcga gcacgtatta
      121 cgagaaaccg ctcacaccaa acgaagttaa cgcttttacg ttcgatggaa agttcctgtt
      181 cttcatcccc gtcttcggca gaggctggga gtttctgctg agacctgcgg agagtttgat
      241 actgagcgcg ggcttgagcc ttctgtacaa tttcctgccc ggtgtgtatt tgaatctaac
      301 gatccttcta taaggcaaac ttttacggaa gaagcggtga gaaatcaccg cttcttttgt
      361 ttaatatacc tgtacccgac ttatagccgg gagatgacgt tccccggttg gcaggtcttc
      421 atgagttatt taaatcgccg tggtaacata gtgaacaagt cttgagttcg aggtgatgat
      481 aatgaatact ataaaagaag atgcgcgtta cataattgag agatccatag agaccgtact
      541 gccggagaag gcggttttca attccctgga gagtctgcat ttacccggaa aaactttcct
      601 gctggctatc gggaaggccg cctggaggat ggctaaagcg gcgagggaga agctcgggca
      661 gtctattacg gccggtgtga tcatcaccaa gtacggacac gttgaaggag acataccggg
      721 aatcgatagt tttgaagccg ggcaccctct tcctgacgaa aacacaatcg ccgcaacgac
      781 gaaagctctg gaaatgataa acgccatcaa ccccgacagt atattgtttc tgatctccgg
      841 gggcggttcg gctctcttcg aaaagcccgt ggagggagtt actctggatg atctggtgga
      901 tataaacaac cgcttattga ggtcgggagc gaacatcgtc gagatcaacg cggttagaaa
      961 gcgtctatcg gtagtaaaag gcggacgttt cgcgaagatg ttcgctcaaa ctacgataca
     1021 ttcactggta ctctccgatg ttcttggaga tagactggat tcgatcgcct ccggtccggc
     1081 ataccccgac agcagtacct gcgaggatgc gctatccaca attgataggt acggtctggt
     1141 tctcagaccg gaactccgcg agatcttgaa aatcgaaacg ccgcatcttt taagtaacgt
     1201 tcaaaccaga atcataggga gtgtcggcag ggcctgcgaa gctgccggcg tctttgccaa
     1261 agagctcggt tacgagccca tgatattgac cacaacgctc gattgcgagg ccagagacgc
     1321 gggaagcttc ctggcggcga tagcccgaga agaggtgtat gaccggcctt tgaagagacc
     1381 gtgcgcgata atcctcgggg gagagacagt ggtccccgtc aggggaacgg gacttggcgg
     1441 caggaatcaa gaactggccc tttcattcgc gatcgcgtcg aacgggctgg acaacgttgc
     1501 tctggccagt gtcgggacgg atgggacgga tggtccaacc gacgccgcag gcgcgatagt
     1561 ggatggtggt agtgccagag aaatgatcga gcggggaata gatccacaca tctacctcct
     1621 ggacaacgac tcctatcacg cgctggaaat gtccggtgaa cttctcaaga ccggccccac
     1681 cggaactaac gtcaacgatc tggtagtttt gctttgccgg tgagagacaa gaagatcata
     1741 agcagtcgtt ttcgttcgca actggtctta ccgggggaat tatacttcca ttccgttgta
     1801 tttcgacatg acttttttga agtcgccggc cacgatttcc cgcgcagact gaacggcgag
     1861 atctatcgct ttgctcacgg ttctgtaatc ctcgtctgaa aactgcccga gcacgtaatc
     1921 gatcatatcg cggttctccg ggagaggtcc cacgcctatg cggattcgtg gaaagtcctt
     1981 cgttcccagt tcggtgatta tcgattttaa gccattgtgt cctccgtctg agccattctc
     2041 cctgatcctt atccttccca gagggatcca gatatcgtca tagactatca cgaccgtatc
     2101 ctcccgggtg aggttaagat ccgcgatcag actcttcacc gcgataccgc tgagattcat
     2161 gaaggtcagt ggtttcgcca gatatatctt gggctctccc tccagaagat aggcttcgta
     2221 aatcttcttt ttggaggtct tccggactcc attcctcatc atctcgtcca cgaagagaaa
     2281 acccacgtta tgtctggtga aaacataacg tgggccggga tttcccagac ctataaaaag
     2341 gaacactatt tattcctctt cttcctcttt ctttcccttg tttataactt cgggttcggt
     2401 accctctttt tcttccggcg cttcttctac ttcggctcct ctggctgccc taggaagctc
     2461 tataacaaga actggctctt cgccgtcagc caggggcttc attccttcgg gaatcggaag
     2521 atctttgact ctcagtatct tgccgacatc gaaagaagag atatcgacat ctatcgattc
     2581 aacgatatcc tttggaagca cagagacggg aagctcgttg attatatgtt caacgattcc
     2641 acctttttca atgccgatcg gctttccgct cagccttatc ggaatgtgaa tctccattct
     2701 gcgacctttg gatggagagt agaaatccag gtgtataatc gcgtcggtca ccttgtttct
     2761 ttgaacggat ttaaggaaga cgtggtgttc tttgccgtcc agggatagaa tgacagaagt
     2821 tgtttcggca atcttgttta tatttctgag cacctcaact ttgtcgaggc ttacagagaa
     2881 agtctcaact tctggtccgt aaactaccgc tggaatcttt ccctgattca
//
LOCUS       DAOOLY010000288         2920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_41530_length_2920_cov_3.870157, whole genome shotgun sequence.
ACCESSION   DAOOLY010000288 DAOOLY010000000
VERSION     DAOOLY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41530_length_2920_cov_3.870157"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLD15_11170"
     CDS             <1..853
                     /locus_tag="PLD15_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPB64516.1"
                     /translation="YVIKTFTGQKFPQGELVLDGANGAIATVVEPVYHALDINARFLG
                     MEPNGININDGCGSLHPEFIGAALGEMEVGILFDGDADRCLFVLQGSRVVDGDMLMAI
                     NSRKMVRQGRLKGNRVVATVMSNLGFEKYLSGRGIGLERTKVGDKYVLERMLQTGGVL
                     GGEQSGHIIFLDRSTTGDGLITSLETLNTLSELGETLEGFLKEFPVFPQLLKNVPVSD
                     KKGVMECEKLKARLYELESWRDLRIVLRPSGTEPYVRVMVEGVDSREVEETCQELVEL
                     VEECSNG"
     gene            846..>2920
                     /gene="uvrA"
                     /locus_tag="PLD15_11175"
     CDS             846..>2920
                     /gene="uvrA"
                     /locus_tag="PLD15_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPB64517.1"
                     /translation="MDRYISVKGARVHNLKNVSVEIPKNSLTIITGLSGSGKSSLALD
                     TIYAEGQRRYLESLSTYARQFLGELKRPDVESIEGLSPAIAIEQKTVSHNPRSTVGTV
                     TEIHDHLRILYARVGIPHCPSCGRAVQRQSLDEIVEGIFKEFSEGSRIAIYSPICKEK
                     KGEYKKELENLRKKGYVRVEIDDHVYDLEEDLKLDKNKRHTISLVVDRLKVERENASR
                     IADSVEMALREGNGFVEIGLIDAKERKIFSENFACPVCGISLPEISPKIFSFNNPFGA
                     CPRCHGLGYTMEFSRDLVVNEDLSVLKGSFKAISGSQDSFTMKMLIRVIESLGESPDK
                     PFKDLREDVKNALLFGTTDDITMKYKSEKLTYELKRPYEGVINNLQRRYQETQSEEMR
                     YWMESSFMVQQTCTQCNGQRLRPEALAVTLKDRNIASVSDMTIQELFDFVQEIHLTEH
                     QFEIVGELMGEIEKRLKFLVDVGLDYITLSRYAMTLSGGESQRIRLATQIGSGLTGVT
                     YVLDEPTIGLHSRDNDRLIKTLKNLRDLGNTVIVVEHDEEVIRSSDYIVDVGPGAGVH
                     GGEIVYQGPTQRLIEDPPERSLTGKYLKGECSVPRLEVAGNGDRGWLTIKGASRNNLK
                     NIDVAFPLGRFVTVTGVSGSGKSSLVMDTVYPSLKNRLGNSKNPVNTEVSLEGWEAID
                     NVIVIDQSP"
BASE COUNT          806 a          700 c          809 g          605 t
ORIGIN      
        1 ttacgtaata aagaccttca ccggtcaaaa gttcccgcag ggcgagctcg ttctcgatgg
       61 cgcaaacggg gcaattgcaa cggttgttga acccgtttat catgcgctcg acataaatgc
      121 ccggtttctc gggatggagc ccaacggcat aaatatcaac gacggatgtg gctccctaca
      181 tcccgagttt ataggggcgg ctcttggaga aatggaggtt ggaatcctct tcgacggcga
      241 tgctgacaga tgcctcttcg ttcttcaggg ttcgcgtgtc gtagatggcg atatgctaat
      301 ggccatcaat tctagaaaga tggtcagaca gggtaggttg aaaggaaacc gcgtagtggc
      361 taccgtcatg tctaacctcg gtttcgagaa gtatctttcc ggcagaggaa tcggcctgga
      421 gagaacgaag gtcggcgata agtacgttct ggagagaatg ctgcagaccg gtggcgtttt
      481 gggcggagag cagtccggac atatcatatt cctagataga agcacgaccg gagacggtct
      541 gataacttca ctggaaacgt tgaacactct gagcgagctc ggcgaaacgc tcgagggttt
      601 tctaaaagag ttcccggtgt ttccccagtt gctcaagaac gtacctgtct ccgataaaaa
      661 aggcgtcatg gagtgtgaaa aactcaaggc aaggctctac gaacttgaaa gctggaggga
      721 tttgcggata gtcctgcgtc catcgggcac cgaaccctat gtgagagtaa tggtcgaagg
      781 cgtcgattca cgagaagtgg aggagacctg tcaggagctc gtcgaactcg ttgaggagtg
      841 tagcaatgga taggtacata tctgtcaaag gagccagagt ccacaatcta aaaaacgtga
      901 gcgtcgaaat acccaagaac tctctcacga tcataaccgg cctttctggc tcgggcaagt
      961 cttcgctcgc actggacacc atttacgcag aaggtcagag acggtatctg gaatcgcttt
     1021 caacttacgc cagacagttt ctaggtgaac tcaagagacc cgacgtggag agcatcgaag
     1081 ggctttcacc cgcgatagcc atagaacaga agaccgtcag ccataaccca agatctacag
     1141 tcggaacggt gacagagatt cacgaccatt tgagaatcct ttacgcacgt gtcgggatcc
     1201 cacactgtcc ctcttgcgga agagcagttc agcggcagag tctcgacgag atagtggagg
     1261 gtatcttcaa ggagttttcc gaaggctcca ggatcgccat atactcgccg atttgcaagg
     1321 aaaagaaggg cgagtacaag aaggaactgg agaacctcag aaagaagggt tacgtgcgtg
     1381 tggagatcga cgatcatgtc tacgatctcg aagaggacct gaagctcgac aagaacaaga
     1441 gacacacgat aagccttgtg gtcgacaggt tgaaagtcga gagagagaac gcctcgagaa
     1501 tcgccgacag cgtagaaatg gcgctccgcg aaggaaacgg attcgtggag atcggtttga
     1561 tcgacgctaa ggagagaaag atcttcagcg agaacttcgc ctgcccggtt tgtgggataa
     1621 gtcttccaga gataagtccc aagatcttct cattcaacaa cccctttggc gcctgtccca
     1681 ggtgtcatgg cctggggtac acaatggaat tctccaggga cctggtagtc aacgaagacc
     1741 tgagcgtact aaaaggctcc ttcaaagcca tctctggatc tcaggacagt ttcaccatga
     1801 agatgttgat cagggtcatc gaatcgctcg gggaatctcc cgataagcct ttcaaagatt
     1861 tgcgcgaaga tgtgaaaaac gccttactct tcggcactac cgatgacata accatgaagt
     1921 acaaatcgga aaaattgacc tacgaactga agagacccta cgaaggtgtg atcaacaacc
     1981 tccagaggcg gtaccaggaa acccagtcgg aagagatgcg ctactggatg gaaagcagct
     2041 tcatggttca gcagacatgc actcagtgca atgggcagcg tttacgcccc gaggcgctgg
     2101 ccgtgaccct caaggacagg aatattgcat cggtctcgga catgacgatc caggagcttt
     2161 tcgatttcgt gcaggagata catctaaccg aacaccagtt cgagatagtc ggtgaactca
     2221 tgggcgagat cgaaaagaga ttgaagttcc tggtcgatgt cggcctcgat tatataacgc
     2281 tctccagata cgcgatgaca ctatccggcg gggagtccca aagaataagg ctggccacgc
     2341 agatcggctc gggtctcaca ggagtgacct atgtactgga cgaaccgacc atcggtctcc
     2401 attcccgcga caacgacaga ctgatcaaaa ctctcaagaa cctgcgcgac ttagggaaca
     2461 cggttatagt ggtcgaacac gatgaagagg ttataagaag ctccgattac atagtagatg
     2521 tggggccggg agcgggtgtt cacggcggag agatagtcta ccagggtccc acgcagagac
     2581 tcatcgaaga ccctcccgaa agatctctaa caggaaagta cttaaaaggc gagtgttcgg
     2641 tccccagact tgaagttgcc ggcaacggcg atcgtgggtg gttgaccatc aaaggggcat
     2701 cacgcaacaa cctgaaaaac attgatgtcg cctttcccct gggaaggttc gtgaccgtca
     2761 caggggtctc tggtagcggt aagagttcgc tggtaatgga cacggtttac ccctctctaa
     2821 aaaaccgtct gggcaactcc aaaaacccgg taaataccga agtcagcctc gaaggctggg
     2881 aagcgattga caacgtaata gtgatagatc agagccccat
//
LOCUS       DAOOLY010000289         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_42268_length_2882_cov_5.077114, whole genome shotgun sequence.
ACCESSION   DAOOLY010000289 DAOOLY010000000
VERSION     DAOOLY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42268_length_2882_cov_5.077114"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1561)
                     /gene="gcvPB"
                     /locus_tag="PLD15_11180"
     CDS             complement(113..1561)
                     /gene="gcvPB"
                     /locus_tag="PLD15_11180"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPB64518.1"
                     /translation="MTIFDATVEGRTGFRLPEEKSYGFEPIDVLPEHLLRDSRPELPQ
                     VSELQVVRHFTGLSRKNHSVDSGFYPLGSCTMKYNPKLNEEAASFEGFSQLHPYQPEE
                     TIQGALELMYNLQNSLCEITGMDDFTLQPAAGAHGELVGMLLVKKYFELKGDTSRKKV
                     IVPDSAHGTNPASAAMAGYDIVEVASNKDGRVDLESLDRLLDESVAAIMLTNPNTVGL
                     FEKDICEIQKMAHDRGALLYYDGANLNAIMGHARPGDMGFDIVHLNLHKTFSTPHGMG
                     GPGSGTVGVKTFLKELLPVPVIDFDGQKYRIVYDLPDSIGKIRSFFGNFGVLVRAYAY
                     IKTMGSDGLKRASEMAVLNANYLRSKLVELIPTAYSGICKHEFVLKGTKLISEYGIRT
                     LDLGKRLLDYGIHPPTVYFPLIVDEAIMIEPTETETKETLDHFAATIATILEEAKENP
                     ELLKLAPHTTPVGRLDEATASRKPKTRWSKGS"
     gene            complement(1558..>2882)
                     /gene="gcvPA"
                     /locus_tag="PLD15_11185"
     CDS             complement(1558..>2882)
                     /gene="gcvPA"
                     /locus_tag="PLD15_11185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPB64519.1"
                     /translation="QTEVEIEEMLRAIGVKSIAELFTDIPEKFDVDLSIPASADEFTV
                     LRDLKELSQRNSNLNDLSVFRGGGIYKHFIPSAVQRIAGRGEFLTAYTPYQAEISQGT
                     LQMLFEYQTMISELTGMEVSNSSMYDGASACAEAALMSVRINGKTKILISQAVHPEYI
                     DTVKTYCFGGNIQVDEVNFDRKSGQIDLSDLKSKLSDDVSAVIVGYPNFFGVIEDLRE
                     VRKAIPENVMMIVSANPTALAILRAPGKLGADIVVGEGQPLGNPPYLGGPGFGFFASK
                     EAYIRKMPGRIIGETKDGEGRTGYVMVLQTREQHIRRNKATSNICSNQAFNVLLASIY
                     MSLVGPKGLQEIARRSYDKAHYLAKRIEKMDKVRPVFTGPFFSEFVVEFKKDLSSLNS
                     KLLKDKILGPLDLGEFRDDMKNHGLICLTEANRNEEIEFFAGRLEELE"
BASE COUNT          645 a          768 c          668 g          801 t
ORIGIN      
        1 cgatgagact tgaactcata acctccgcct taccaaggcg gcgctccacc gattgaagct
       61 acgcctgctc tgctgaaact atgatatcat agcattttct aactttcaag cctcaagaac
      121 ccttagacca ccttgtcttt ggctttcttg aagccgttgc ttcatcaaga cgccctactg
      181 gtgtagtgtg aggcgccaat ttcaacagtt ccgggttttc cttcgcttcc tcgagtatag
      241 tcgctatcgt ggccgcgaag tgatcgagtg tttccttagt ttcggtctct gtcggttcta
      301 tcataatggc ctcatcgacg atgagcggga aatagacggt cggtggatga ataccgtaat
      361 ccagaagtct cttgccaaga tcgagagttc tgataccgta ctcggagata agctttgtgc
      421 ccttgagaac gaactcgtgc ttgcagatgc ccgaataggc ggttggtata agttcaacca
      481 gcttgctcct caggtagttg gcgttcagca cggccatttc gcttgctctt ttcaaaccgt
      541 cgctgcccat ggttttgatg taggcataag cccttaccaa gacaccgaag tttccgaaga
      601 aactcctgat cttccctatc gaatctggca gatcataaac tatcctgtat ttctgaccgt
      661 cgaaatctat caccggaacg ggtagtagct ctttcaaaaa ggtctttact cctaccgtgc
      721 cacttccggg cccccccatt ccgtgaggcg tggaaaaggt tttgtgcaaa ttgaggtgaa
      781 cgatatcgaa gcccatgtcg cccggcctcg catgtcccat tatcgcgttg aggttcgccc
      841 catcgtagta aagcaaagct cccctgtcgt gagccatctt ctgtatttca cagatatcct
      901 tctcgaagag cccgacggtg ttcggattgg tgagcattat tgccgcaaca ctttcatcta
      961 gcagtctgtc caggctttca agatcaaccc taccgtcttt gttggaggcg acttcgacta
     1021 tatcataacc ggccatcgcc gccgaagccg ggttggttcc atgtgcagaa tccggtacta
     1081 tgactttctt ccgggaagta tcgcccttca attcgaagta cttcttgacc agcagcattc
     1141 ccaccagttc gccgtgtgca ccggcagctg gctgcagagt gaagtcatcc ataccagtga
     1201 tctcgcacag cgaattctgc agattgtaca tcaactccag agctccctgt atcgtctctt
     1261 cgggttgata gggatgtaac tggctgaacc cttcgaaaga ggccgcctct tcgttcagct
     1321 tggggttgta cttcatagtg cacgatccaa gcgggtagaa gccgctatct accgaatgat
     1381 tctttctgga aagacccgtg aagtggcgca ccacctgaag ttcgctcacc tgtggcagtt
     1441 ctggcctgga gtctctcagg agatgttcag gcagaacgtc gatcggctca aaaccgtaac
     1501 ttttttcttc gggaaggcga aatccagttc taccttccac agtcgcatcg aaaatcgtca
     1561 ttctaactcc tccagtctcc cggcgaagaa ttcgatctct tcatttctgt tggcctcggt
     1621 aaggcatatc aaaccgtggt tcttcatatc gtctctgaac tctcccagat cgagcgggcc
     1681 gagtattttg tctttcaaaa gcttcgaatt caaactcgaa agatctttct tgaattcgac
     1741 tacgaactcg ctgaagaaag gtccggtgaa aaccggtctc accttgtcca tcttctctat
     1801 acgtttggcc agatagtggg ccttgtcgta agagcggcgg gcaatttcct gcaaaccttt
     1861 cggtccgacc agactcatat agatcgatgc aaggagcacg ttgaaagcct ggttagaaca
     1921 gatattagat gtggctttat ttcttcttat gtgttgttcg cgagtctgta gaaccataac
     1981 ataaccggtt cttccttcgc cgtctttcgt ttcgccgatt attctgccag gcatctttcg
     2041 tatataagct tctttcgaag cgaaaaagcc gaagcccggt cctccaagat acggcggatt
     2101 gcccagcggc tgaccttctc caacaactat atcggctccg agctttcccg gcgcccggag
     2161 aatcgcaaga gctgtcggat tggcacttac gatcatcata acgttttccg gaatcgcttt
     2221 tcttacttct ctcagatctt cgatgacacc gaagaaattc ggatagccga ctattacggc
     2281 cgatacatcg tcggaaagtt tgctcttaag atctgaaagg tctatctgac cggatttacg
     2341 atcgaaatta acctcgtcaa cctgtatgtt tcccccgaag caatatgttt tcaccgtatc
     2401 gatgtattcc ggatgaacgg cctgcgatat taggattttc gtcttgccat ttatcctaac
     2461 ggacatcaaa gctgcctctg cacaggcgct ggcaccgtcg tacatcgagg agttggagac
     2521 ctccattccg gtgagttcac tgatcattgt ctggtattcg aagagcatct gcagggttcc
     2581 ctgagagatc tcggcctgat agggagtata tgcggtgagg aactccccgc gtcctgctat
     2641 tctctgcacc gcgctgggta tgaagtgctt gtatatacct ccgcctctga atacggaaag
     2701 gtcgttcaaa ttcgagtttc gctggctcag ttctttgaga tccctgagca cagtgaattc
     2761 gtctgcgctg gccggtatcg aaagatcgac atcgaacttt tccggaatgt ccgtgaagag
     2821 ttcggcaatg cttttgacac ctatagcccg cagcatctcc tctatttcta cctctgtctg
     2881 ag
//
LOCUS       DAOOLY010000290         2876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_42396_length_2876_cov_4.794754, whole genome shotgun sequence.
ACCESSION   DAOOLY010000290 DAOOLY010000000
VERSION     DAOOLY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42396_length_2876_cov_4.794754"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLD15_11190"
     CDS             complement(<1..255)
                     /locus_tag="PLD15_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64520.1"
                     /translation="MRRILSILIFFLAAFSALSVEKIVVAVMQDPDFLDPHRAAASGT
                     YEMMFNVFEGLLKPDSKGNVVPAIAESYSISPDGLTYTFKL"
     gene            620..1063
                     /locus_tag="PLD15_11195"
     CDS             620..1063
                     /locus_tag="PLD15_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB64521.1"
                     /translation="MPDANFRVRAMSESPARVSVKARSFNMIVDEPPNLGGEDRGANP
                     VEYVLAALAGCLNVVGHMVAGEMGFSIKKLEIDVSGPLNAARLMGKSQDDRAGSKDIT
                     ATMKVDSDADEETLERWVKVVEDRCPVSDNLSNETPLSVVFSTIG"
     gene            complement(1120..2721)
                     /locus_tag="PLD15_11200"
     CDS             complement(1120..2721)
                     /locus_tag="PLD15_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPB64522.1"
                     /translation="MRRYAFLPVVSLMIIFLTGCPKVDIHYSLSGVIRPYSGEEGFTV
                     QRKGYVPELTAEDIERGFTDEYAVIFDSGYDSTILPGAEVLGEFSSRDGSVVISTVRT
                     SDPQALAAIEGVRSVQRNMLYTLQSVTTVPDDPLYTDQWGYPMVQMEEAWTIAKGSPT
                     VVVAIIDSGTRPDHPDLQGVFLPGYDLIDDDDDPTDNPNPGRQSHGTHVTGTITAVTN
                     NGTGVSGMTWGRYPTVMPIKVFDNGGNTTDTIIAEGIIYAVDHGAKVINMSLAGPDTE
                     AVRLAVEYAYANEVMMIAAAGNYNTSYPYYPASYDEVISVSAVGPTMERASYSNYGDS
                     IDFAAPGGDSKVGGQTGTIISTGYTSAGGNGYTYLSGTSMACPHVTGLVALLMGEGYI
                     GRDSNGEEIIRKIIRKTALDLGDPGWDEYYGYGLIQAANALSFIEEKRPFVVQVLSIG
                     GSVLAQSDVRPDGSFKIGGLKDQNIKLRVWRDVNANGVIDKGDLVGYAGYSGWEPVFE
                     NAALLFYNSSGEKELAGPVNFSPVM"
BASE COUNT          673 a          770 c          738 g          695 t
ORIGIN      
        1 gagcttgaaa gtgtatgtga gaccgtcggg agagatggaa tagctctcgg cgatcgccgg
       61 aaccacgttc cccttcgaat cgggtttcag gagaccttcg aacacgttga acatcatctc
      121 gtatgttccc gaggccgccg ccctgtgcgg atcgagaaag tccgggtcct gcatgacggc
      181 aacgactatt ttctctaccg aaagcgcgct aaaggcggcc aaaaagaaaa tcaaaattga
      241 aagaattctt ctcatatact ttcctccttc tggagttggt ttatcgtgta tctccctttc
      301 gtacagatgc tacctatata ttaccgggtg ttcttttccc cagttataaa tacctgtgtt
      361 tctctcaaca accttcgttc ttacagacat tatcataaac ccggcagatt gaccacagaa
      421 attcccactg tcttcggaca cgttatttag tatcgcttcg ctcataagaa gtcgggaatc
      481 ctcttaagaa atgttgttgg ttgtgcgtgg tgcgttgtgg gtaagggtat agaaacgata
      541 atttatgtcg gcttaacatt cggcacgctg aaagaatgta taataaatgt gaaagaaatc
      601 acaacaggag gtataaaata tgccggatgc caattttaga gtaagagcga tgtcggaaag
      661 tcccgcgagg gtttcggtga aagccagaag cttcaacatg atagtggacg aaccgccaaa
      721 cctgggcggg gaggaccggg gagccaaccc ggtagagtac gtactggccg ccctggccgg
      781 ctgtctcaac gtggtgggtc acatggtggc cggagagatg ggcttttcta taaagaaact
      841 cgagatagat gtctccggtc cgctgaacgc ggccaggctt atgggaaaat cccaggacga
      901 cagggccggc tccaaagata taacggccac tatgaaggtc gatagcgacg ccgacgaaga
      961 aactctggag agatgggtaa aagtggtcga ggatcgctgt cctgtatcgg acaacctttc
     1021 gaacgagact cccctcagcg ttgtgttttc gactataggc tgagcgccat ttatacgggc
     1081 aatcacaaag aaagagactc ccgatcggga gtctcttttt tacattacag gtgagaagtt
     1141 gaccggtcct gccagctcct tttctccgct ggaattgtaa aagagcaggg cggcgttctc
     1201 gaagaccggt tcccatccgc tatatccggc gtagccaacc agatcgccct tgtctatgac
     1261 tccattcgcg ttgacatcac gccagaccct caacttgatg ttctggtctt tcaagccgcc
     1321 gatcttgaaa gagccgtccg gcctgacatc gctctgggcc agcaccgagc ctccgatgct
     1381 caacacctgc accacgaaag gtctcttctc ctcgatgaag gacagggcat ttgcggcctg
     1441 aatcaatcca taaccgtaat actcgtccca tcctggatcg cccagatcca gcgctgtctt
     1501 cctgattatc ttcctgatta tctcttcgcc gttcgaatcc cttcctatgt atccctctcc
     1561 catcaggagc gcgaccaggc cggtcacgtg cggacaggcc atggaagttc cggaaagata
     1621 ggtgtaacca tttcctcccg cggaagtgta gcccgtactg ataatcgtac cggtctgacc
     1681 tccgacttta gaatccccgc ccggggcggc gaaatctatg gagtctccgt agttcgaata
     1741 actggccctt tccatggtcg gcccgaccgc gctaaccgag atgacttcat cgtaagaggc
     1801 cggatagtac ggataggaag tgttgtagtt accggctgcc gctatcatca tcacttcgtt
     1861 ggcgtaagcg tactccaccg ccagcctcac ggcctcggtg tccggtccgg ccaggctcat
     1921 gttgatcacc ttcgccccgt gatccacggc gtagattatc ccctcggcga tgattgtatc
     1981 ggttgtattt ccaccgttgt cgaagacttt tatgggcatc acggtcggat accttcccca
     2041 ggtcattccg gacacgccgg tgccgttgtt tgtaacggcc gtgatcgttc cggttacgtg
     2101 tgtaccgtgg gattgtcgac cggggttcgg gttgtcggtc ggatcgtcat cgtcgtcgat
     2161 cagatcgtac ccgggaagaa acactccctg caggtcggga tggtccggtc tggtaccgga
     2221 gtcgataatg gccacgacca ccgtcggaga acctttagcg atagtccacg cctcctccat
     2281 ctgtaccatg ggataacccc actgatctgt gtagagagga tcatccggca cggtagtcac
     2341 cgattgcaaa gtgtacaaca tgtttctctg cacggacctg acgccttcga ttgcggcaag
     2401 ggcttgcgga tcggatgtcc tgacggtcga gatgaccacc gaaccatcgc gcgaggagaa
     2461 ttcgcccaga acctccgccc ccggcagtat cgtcgaatcg taaccgctgt cgaagatcac
     2521 tgcgtattcg tcggtgaagc ctctttcgat atcttcggcc gtcagctccg gaacataacc
     2581 ctttctctga accgtgaacc cttcctcacc tgaatatggc ctgataaccc cgctgaggga
     2641 ataatgaata tccactttgg gacagcctgt cagaaaaatg atcatcagtg aaacaaccgg
     2701 aagaaaagcg tatcttcgca tcatgaacct ccatcgatat cataatgaag attttatcat
     2761 ctcagctacg tgagtttccg ttgaaaccgg agagcggtta agtgttgtat gaaaaagctc
     2821 gctgttcagg ggcggaacag ttggtagtag tgataaagct cctgtaaccc cgcctt
//
LOCUS       DAOOLY010000291         2857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_42791_length_2857_cov_2.374019, whole genome shotgun sequence.
ACCESSION   DAOOLY010000291 DAOOLY010000000
VERSION     DAOOLY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42791_length_2857_cov_2.374019"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLD15_11205"
     CDS             complement(<1..452)
                     /locus_tag="PLD15_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64523.1"
                     /translation="MRKNDIENIAKILRKRALVRTGTIVGFENDGKVVQVRFHDTKLP
                     EGQFERKLTCEGIIEGSTFTLIQPNFRRPVSDLVDVEKEVGAGSFKGLTLPLVGFETY
                     PEGSENPDSIIGLFELPGGSIYKEVLTEASHDILIPGYGTFSSQKVIG"
     gene            complement(452..1840)
                     /locus_tag="PLD15_11210"
     CDS             complement(452..1840)
                     /locus_tag="PLD15_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64524.1"
                     /translation="MAFVENRYVIEVRTSSGYLLVDSVQGSERAGLTPFTGSFRCRLS
                     DPSLVSKGQTVTLELWHNSRLDGGSFVETIAKVEIERVRKTTNAMSHVTVEAEFKDKL
                     ARYLEQETDILMFSLDPGLTPAGMAQAIYLGTPYQADTSPIEASESNLRARDFQISGS
                     LRDATIKLERILGARTLIDHSSKTVRFIPDYHDSSSITLEGVMEKTEIEAGDVSSGVK
                     LTGFLNTIPLEKGLVRHYNLRGESWSVILTKELYTTESEQQRVKYTGKCRTSVRIDEI
                     DKIESISTITYGFDHISLDHLYTEEEYDLLEEEPSGDPIYKMYLVWSPESNEVSIYRT
                     IILDYVAGSSWDWGESRTLWKMKKVCEKYIGLYLRGKVFKFPIELQVGPKPREHYSTN
                     EPASAVAALRLANLLYAKKSYQKETKIEVTPSKYIRPGGRATLGGHSYVIDGVSFSSH
                     PYEVSLSLLEAI"
     gene            complement(1840..2607)
                     /locus_tag="PLD15_11215"
     CDS             complement(1840..2607)
                     /locus_tag="PLD15_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64525.1"
                     /translation="MSGFYKIFEENAGKTGEFGDALKEIEPELQSFASSLKEIEVEFD
                     ETWSNIISASKNGVDAVIEEFRRASDEIVGHSIVPEMKAEIIREMVELATGMRDEASR
                     GAANSMASFADLSDGLRDYLSLMQREASSFSMDSITTVAAEFPEYFSNLSHEAAKAVG
                     NMKEFYDLRWLDDVYRGTTDKTRDMSYTFAQLQDQLRDLSGELGDFIPSGADLSVPKI
                     ERKKEYHIYLDIKKEEYADKDELIRRIARELELAGEI"
     gene            complement(2541..>2857)
                     /locus_tag="PLD15_11220"
     CDS             complement(2541..>2857)
                     /locus_tag="PLD15_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64526.1"
                     /translation="EAAELLAPDLAPLNEVAARLRLEGGRLALEQIRARLGGGALEGT
                     LGLDVAAVPPVLSGSLGLTGATLTRQDLWQRKFRRKRMRCPGFTRSSRKTPERPASLV
                     MR"
BASE COUNT          601 a          823 c          660 g          773 t
ORIGIN      
        1 tcccctatca ctttctggct cgaaaaggtc ccgtacccgg gaatgaggat gtcatggctc
       61 gcttccgtaa ggacttcttt gtatatggag cctccaggga gttcgaagag tccgataatg
      121 ctgtccgggt tttctgaacc ctcagggtag gtctcgaatc ccacaagtgg cagcgtgaga
      181 cccttgaaac tgcccgcccc gacttctttc tccacatcaa ctagatctga gacgggcctc
      241 ctaaaatttg gttgtatcag ggtgaaagtg cttccttcga ttatcccttc acaggtgagt
      301 tttctctcga actgtccttc agggagcttc gtatcgtgga atcgaacctg cactaccttg
      361 ccgtcgtttt caaatcccac gatcgtgccg gtccttacca atgccctttt ccttagaatc
      421 ttcgcaatgt tctctatgtc attctttcgc atcaaatcgc ctccagaagg gaaaggctta
      481 cctcatacgg gtgggaagag aacgagactc cgtcgatcac atatgaatgc ccaccaagtg
      541 tcgctcttcc ccccggtctt atgtacttag acggcgttac ctcgatcttc gtctctttct
      601 gatagctctt cttcgcatac agcagatttg caagtctcaa agcagccacc gcagacgctg
      661 gctcattggt gctgtaatgt tctctaggct tgggcccgac ctgaagctcg atcggaaact
      721 tgaagacttt tcctctcagg taaagcccaa tatacttctc acataccttc ttcatcttcc
      781 agagagttct tgactctccc cagtcccaag aggagccggc aacatagtca agaatgatcg
      841 tccgataaat cgagacttcg tttgattcgg gactccagac caaatacatc ttgtatatgg
      901 gatcaccgga gggctcttct tccaggagat cgtattcttc ttccgtgtaa aggtgatcga
      961 gggaaatatg gtcaaagccg tacgttattg tgcttatcga ctcgatcttg tctatttcgt
     1021 ctattcttac gcttgtcctg cacttgcccg tgtacttcac gcgttgttgt tcgctttcgg
     1081 tcgtgtacag ttctttcgtc aggatcaccg accacgactc gccccggagg ttgtaatgcc
     1141 ttaccagccc cttttctaaa ggaatcgtat tcagaaaccc agtaagcttc actccagatg
     1201 aaacgtcgcc agcctctatc tccgtcttct ccatgacacc ttcaagggta atggaagaag
     1261 agtcgtggta gtcgggtatg aacctcacag tctttgacga gtggtcaata agggtccttg
     1321 cacccaggat tctctcgagc tttatcgttg cgtctctcaa tgaaccactt atctgaaaat
     1381 ctcttgctct cagattactt tctgacgctt cgattgggct agtatccgcc tgatagggag
     1441 tccccagata tatcgcctga gccattcctg caggtgtcag tccgggatcg agagagaaca
     1501 tgaggatgtc agtttcttgc tcgagatatc tggcaagctt gtctttgaat tccgcttcga
     1561 cagttacgtg gctcatggcg ttcgttgtct tcctgactct ctcgatctct actttggcaa
     1621 tagtctctac aaatgagcct ccatccagtc tcgagttgtg ccagagctct agagtcaccg
     1681 tctgtccctt cgaaacgagc gacggatcgg aaagtcgaca gcgaaaagag ccggtgaaag
     1741 gagttaagcc ggctctctcg ctgccctgca ccgaatcgac aaggaggtaa ccactgctcg
     1801 ttctcacttc gattacgtat ctattctcaa cgaaggccat cagatctcac ctgccagttc
     1861 cagttccctg gcaatcctac ggatcagttc gtctttgtcc gcgtactctt ccttctttat
     1921 gtcaaggtag atgtggtact ctttcttcct ttcaatcttc gggacgctga gatcagcgcc
     1981 cgaaggaatg aagtctccga gttcaccgga aagatctctg agctgatcct gcagttgtgc
     2041 gaaggtatat gacatatctc ttgtcttatc agtagtaccc ctgtaaacgt cgtctagcca
     2101 tcgaaggtcg tagaattcct tcatgttccc cacggctttg gccgcttcat gtgagagatt
     2161 cgagaagtat tctgggaatt cggccgcgac cgtcgttatg ctgtccattg agaatgaaga
     2221 ggcctctctt tgcatgagtg aaaggtaatc cctcagaccg tcagaaagat ccgcgaacga
     2281 tgccattgaa ttggcggcac ctctcgaggc ctcgtctctc atccctgtgg caagctctac
     2341 catttcacgg attatctctg ccttcatctc aggcacgata gagtgtccaa ctatctcgtc
     2401 gcttgctcgt ctgaactctt caataaccgc gtctaccccg ttcttcgatg ccgagattat
     2461 gttcgaccag gtctcgtcaa attcgacctc tatctccttc agcgaagatg caaaggattg
     2521 gagttccggt tcgatctcct tcagcgcatc accaaactcg ccggtctttc cggcgttttc
     2581 ctcgaagatc ttgtaaaacc cggacatctc atccgtttgc gcctgaattt tctctgccag
     2641 agatcctgcc gcgtcagcgt cgccccggtc aggcccagcg agcctgacag caccggcggc
     2701 accgccgcca catcaagccc gagcgtgcct tccagcgccc cgccgccgag ccgcgcgcgg
     2761 atctgttcca gcgccagccg cccgccctcc agccgcagcc gcgcggccac ctcgttcagc
     2821 ggcgccaggt cgggggcgag aagctcagcg gcctcga
//
LOCUS       DAOOLY010000292         2833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_43328_length_2833_cov_4.205544, whole genome shotgun sequence.
ACCESSION   DAOOLY010000292 DAOOLY010000000
VERSION     DAOOLY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43328_length_2833_cov_4.205544"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLD15_11225"
     CDS             complement(<1..861)
                     /locus_tag="PLD15_11225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730760.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPB64527.1"
                     /translation="MKISPSEITKVIQDQLQKTDIEFDYFEAGKVIQIGDGIARAYGL
                     KGVMANELVKFENGVFGLALNLEEDNVGVVILGSYKDIKEGDLIRRTGRIMEVPTGKA
                     LLGRVVNPLGIPLDGKGPIESDSYRPIEVKATGVVMRKPVDTPLQTGIKAIDATVPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKDQNVKCIYVAIGQKTAALAKILDRLNQFGAM
                     EYTTIVFASASDTAALSYLAPYAGAAMGESFMFLGQDALVIYDDLSKHASAYRELSLL
                     LRRPPGREAYP"
     gene            complement(858..1412)
                     /gene="atpH"
                     /locus_tag="PLD15_11230"
     CDS             complement(858..1412)
                     /gene="atpH"
                     /locus_tag="PLD15_11230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPB64528.1"
                     /translation="MKRSLSLAAKYARALIEIISPEKLEGVRRDLKTLSTVISSEELG
                     KFIFDPTVPGEKKEAMLLSFLSDPYNETTLLVKLLVNMKKIWLLSDILLSFEELILEQ
                     SGRVSVEVISAIPLSDEERVEIIEKVREMTGQEGMLNTRVDESIIAGYVIKFHDEVID
                     ASLSGRLKRVSMGLGNTGSREVIE"
     gene            complement(1409..1894)
                     /gene="atpF"
                     /locus_tag="PLD15_11235"
     CDS             complement(1409..1894)
                     /gene="atpF"
                     /locus_tag="PLD15_11235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPB64529.1"
                     /translation="MIEINLTAVLSILNFLLLYFVLKKVLFDKFFDIIKQRKEKIKGE
                     IAQAEKLRKEANALKEEYTRKMDEARTVSDEMLSRAERQSEEIVRQAREKAQQEIQRM
                     YQAAEIQINQEREKAMEDVKGAVVASAVTMVGRFLQKEMDETARKQYAKRMLESLGDK
                     E"
     gene            complement(1928..2173)
                     /locus_tag="PLD15_11240"
     CDS             complement(1928..2173)
                     /locus_tag="PLD15_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HPB64530.1"
                     /translation="MEIGEGLMMLGKYIGAGLAMGIGAIGPGIGEGRIGASAMEAMAR
                     QPEMIGTLTTRMILADAIAETTGIYSLVIAFMILLVV"
     gene            complement(2194..>2833)
                     /gene="atpB"
                     /locus_tag="PLD15_11245"
     CDS             complement(2194..>2833)
                     /gene="atpB"
                     /locus_tag="PLD15_11245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPB64531.1"
                     /translation="IMALIIFVATRVKVFKIIPGKKQSLLEVAMEYVYDLVEDSVNKK
                     EFVRPIFNIAASIFLFVLLSNIIGSFPGINAIPTDGGGFKITILSDTWYAPTSDLNMN
                     LMLAGFVFVMSHVFSIKAKGFKSWAKSFFEPAWPMFPMNVIGEIAKPVSHSLRLFGNI
                     MGGGILILIIGYLAKYFIVPVALWGIFGLFFGAIQALIFTILAIAYISSLID"
BASE COUNT          679 a          773 c          586 g          795 t
ORIGIN      
        1 agggtaggct tcacgacccg gtggtctgcg cagaagtagt gagagttctc tataggccga
       61 ggcgtgcttg gaaagatcgt cgtaaatgac aagcgcatcc tgtcccagaa acataaaact
      121 ctctcccatg gccgcgcctg catatggagc cagataagaa agcgcagcgg tatcggaagc
      181 cgaagcgaag acgatggttg tgtactccat cgcaccgaac tggttgagcc tgtccaatat
      241 cttggccaac gctgcagttt tctgaccgat ggccacgtat atgcatttca cgttttgatc
      301 tttctggttg ataatcgtat cgatcgctat cgccgtcttt cctgtctgcc tgtcgccaat
      361 tatcagttct ctctgacctc ttcctatcgg cactgtggcg tctatcgcct ttatgccggt
      421 ctgaagaggt gtatcgacag gtttcctcat gactacaccg gtagccttga cttcgatagg
      481 tctgtaactg tcactctcta ttggaccctt accgtccagc ggtatgccaa gggggttcac
      541 cacccgtcca agtagagcct ttccagtcgg gacctccata attcggccgg ttctccttat
      601 tagatctccc tcttttatat ctttgtaaga gcccagtatc acgacaccca cattatcttc
      661 ttcaaggtta agagccaggc caaaaacacc gttctcgaat ttcacaagct cgttagccat
      721 aacgcccttc agaccgtacg cccttgctat accatcgcct atctgaatga ccttaccggc
      781 ttcgaaatag tcaaactcta tatcagtctt ctgaagttga tcctgtatga ccttggtaat
      841 ttcagatggg ctgatcttca ctcaatcacc tcccgagagc cagtgtttcc cagtcccatg
      901 ctgactctct taagacgtcc agatagactc gcatctatca cttcatcgtg gaactttata
      961 acgtaaccgg ctattatcga ttcgtccact cttgtgttta acataccttc ttgaccggtc
     1021 atttcacgga ctttttctat tatttcgacc ctttcttcat cgctcagcgg tatagccgaa
     1081 atcacttcca ccgatacccg acccgattgt tcgagtatca gttcttcgaa cgagagcagg
     1141 atatcggata gaagccagat cttcttcata ttaaccagaa gtttcacaag gagcgtcgtt
     1201 tcattatatg gatcggagag aaagctcaac aacatggctt cctttttttc acccggtaca
     1261 gtagggtcga agatgaactt ccccagttcc tccgaagaga taacggttga cagagtcttc
     1321 aggtccctcc ggactccttc caacttctcg ggggagataa tctctataag ggcccgcgca
     1381 tattttgcgg ccagagaaag gcttcttttc actctttatc accgaggctt tcaagcattc
     1441 ttttggcgta ctgttttctc gcggtttcat ccatttcctt ctgaaggaaa cgtccgacca
     1501 tagtcacggc cgaagccacc acggctccct taacatcttc catggccttt tctcgctcct
     1561 ggttgatttg aatttcggca gcttgataca ttctctgtat ttcctgctga gccttttcgc
     1621 gtgcctggcg tacgatttct tccgactgcc tttcagccct ggagagcatt tcgtcggaga
     1681 cggttctggc ttcatccatc tttcgagtgt actcttcttt aagagcgttt gcttctttcc
     1741 tcaacttttc ggcttgcgct atctctccct tgatcttttc ctttcgttgc tttattatgt
     1801 cgaaaaactt gtcgaacaag accttcttca acacgaaata cagaagcagg aagttcagaa
     1861 tcgatagaac cgcagtcaaa ttgatctcga tcaagttatc acccctcccc tttggggtaa
     1921 gccggtatta cacaaccagc aggatcataa aggctataac cagcgaatag ataccggtgg
     1981 tctctgcgat agcgtcggcc aggatcatcc tggtagtgag tgtacctatc atctccggct
     2041 gcctggccat cgcctccatg gcgctggcac cgatcctacc ttctccaatt ccaggaccta
     2101 tggcaccgat tcccatggcc agtcccgctc ctatgtactt tccaagcatc atgagtcctt
     2161 ctccaatttc cacagtcaat acctcctcgg taatcaatct atcaacgaac tgatgtaagc
     2221 tattgcaagt atcgtaaaga tcaaagcctg tatcgccccg aagaacagtc caaaaatccc
     2281 ccacagtgcc acgggaacta tgaagtactt ggcaagatag ccaattataa gtatgagaat
     2341 accgccaccc attatattcc cgaagagcct caaagaatgc gaaacaggct tggcgatctc
     2401 tcctatcacg ttcatgggaa acatgggcca ggccggttca aaaaagctct tcgcccagct
     2461 cttgaagccc ttagccttta tcgaaaaaac gtggctcata acaaaaacaa agcccgccag
     2521 catcaggttc atattgaggt ccgaagtggg tgcgtaccat gtatcggaaa gtatggttat
     2581 cttgaatcct cctccgtcag ttggtattgc gtttattccc ggaaagctgc cgataatgtt
     2641 cgacaagagc acgaacagaa agatggaagc ggctatgttg aatataggtc tgacgaactc
     2701 tttcttgttt accgaatcct ctacaaggtc gtacacgtac tccattgcga cttccagaag
     2761 gctctgcttc tttcccggga ttatcttaaa aacctttact ctggtagcaa caaaaatgat
     2821 caacgccatg att
//
LOCUS       DAOOLY010000293         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_43561_length_2822_cov_4.770148, whole genome shotgun sequence.
ACCESSION   DAOOLY010000293 DAOOLY010000000
VERSION     DAOOLY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43561_length_2822_cov_4.770148"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2464)
                     /locus_tag="PLD15_11250"
     CDS             complement(<1..2464)
                     /locus_tag="PLD15_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64532.1"
                     /translation="MSKRLTLVLLLVSMVLTSFAFAVEKGPISDIVYFNVRMKEEIAL
                     KDTSEGMTDIFMYGVPGPVIFGLDQATRDKLDLYTVPSGSWSLLMNNVPNEAPYTLEV
                     AGKEVFNAFAIREVRFAMNFLINRQFVTDEILGGAGFPAFTMATEGQPGTYKYNLLAT
                     KFGFTAEGNETLAIEEITKAMQAAAELPANAGKLEKRADGFWYFNNEPVTIKFIIRVD
                     DPNGRLRLGEYVSNQLEKAGLKVERLLWDRSKAGTTVYGGSDPKDLVWQMYTEGWGAG
                     ATRAFWEHIVAQMYAPWYGYMPGNQTEGYWNYVQDEIDEATMNAYTGNFLTEDEYWEL
                     ALRGLELGLEEAVRIYVVNQQDFFTANAARLVDRFAYGLGDGLNGWSIKTAETQDGVL
                     FVTLFSAMGSLFMDAWDPIGTDGFSSTYSSAIAENLYDPAMFESPASAIQTPLRAIPI
                     EVDTKVRRDEEGNVVGDFDVPANAINYDPVSETWVEVGEGHKAMSMATYEFTFGNLHH
                     GQPTTIADFLYAQGFLNEWSTQEGEDDPLYEQGYSSKMKDAQDTLIGWVIHPDDTITV
                     YFNYNFPASKERVAGWGAPGISVSASGQSVGCAWEIIEALSLMITEGSQSGTTYAITR
                     DPAVTEVDVLSPSCVKDIAAKLKEMIDRKYIPNYVKGFVTEEEVLARYNAALNFIDRY
                     GHAYISNGQYYLAKFDTTANFAELRAFRDPTYPYEKGYWKEYLRATRLRIDHLDVPAL
                     STFGMDINVVVDVSEVLYPDDTAVPATKGSVVVTLITDTGEFSFDATFVGDGLFEAVI
                     PASALEGLAAGSYNIMAIASLEG"
BASE COUNT          626 a          739 c          719 g          738 t
ORIGIN      
        1 ctccctcgag agaagctatg gccattatgt tataggaacc cgctgccaga ccctcgagtg
       61 cgcttgccgg aatgaccgcc tcgaagagac cgtcaccgac aaatgtcgcg tcgaagctga
      121 actctcctgt gtcggttatc agcgtaacta cgaccgatcc ctttgtggcc ggaacagcgg
      181 tgtcatccgg gtacagaact tcggaaacat ctacaaccac gttaatatcc attccgaaag
      241 tagagagggc cggaacgtcg aggtggtcga ttctcagtct cgtcgctctg aggtattcct
      301 tccagtaacc cttctcgtac ggataggtgg ggtctctgaa ggctctcagt tcggcgaagt
      361 tggcggtcgt gtcgaatttg gccaaatagt actggccgtt gcttatgtag gcatgaccgt
      421 acctgtcgat gaagttcaat gcagcgttgt atctggcgag cacttcttcc tcggtcacga
      481 accccttgac gtagttgggg atgtactttc tatcgatcat ttccttcaac ttggccgcga
      541 tatccttcac acagctagga gaaagaacat cgacttctgt gaccgccgga tccctggtga
      601 tggcgtaggt ggtgccggac tggcttccct ctgttatcat aagagagagc gcttctatga
      661 tttcccaggc gcaaccaaca gactgaccgg aggccgatac actgattccg ggtgctcccc
      721 agcccgcgac tctctcttta cttgccggga agttgtagtt gaaatacacg gttatggtgt
      781 cgtcgggatg gattacccag ccgatcaatg tatcctgcgc atctttcatc tttgatgaat
      841 aaccctgttc gtacagagga tcatcctcgc cttcctgggt tgaccactcg ttgaggaagc
      901 cctgggcgta caggaaatct gcgattgtcg ttggttgccc gtggtggagg ttaccgaaag
      961 tgaattcata tgtcgccatc gacattgctt tgtgaccttc accaacctct acccatgttt
     1021 cgctaacagg atcgtagttg atcgcgttgg ccggaacatc gaagtcgccg accacgttgc
     1081 cctcttcgtc tcttctgacc ttcgtatcga cctcgatcgg aatagctctc agaggagtct
     1141 gtatggccga agccggactt tcgaacatcg ccgggtcgta gaggttctcg gcgatagcac
     1201 tggaataggt gctgctgaac ccgtcggttc ctatcggatc ccaggcgtcc atgaacagcg
     1261 aacccatcgc cgagaagagt gttacaaaga gaacaccgtc ttgcgtttcg gccgtcttta
     1321 tcgaccagcc gttcaaaccg tcaccgaggc cgtaggcaaa tctatctacc agcctggctg
     1381 cgtttgcggt gaagaagtcc tgctggttga caacgtatat tctaactgcc tcttcgagtc
     1441 ccagctctag acctctgaga gccagttccc agtattcgtc ttcggttagg aagtttccgg
     1501 tgtaagcatt catggtagcc tcgtcgatct cgtcctgaac gtagttccag taaccttcgg
     1561 tctggttacc aggcatgtag ccgtaccatg gagcgtacat ctgggccact atgtgttccc
     1621 agaaggctct cgtcgcacct gcgccccagc cttcggtgta catctgccag accagatcct
     1681 tgggatcgct tccgccgtag accgttgttc cagccttgct tctgtcccag agaagtctct
     1741 cgaccttcaa accggccttt tcaagctggt tggaaacgta ttctccgagt ctcagacgac
     1801 cgttgggatc atcgactctg attatgaact ttatggtgac cggttcgttg ttgaagtacc
     1861 agaaaccatc ggcccgcttc tcgagctttc cagcgttagc cggaagctcc gcggcagcct
     1921 gcatggcctt ggttatctct tcgatcgcca gagtctcgtt tccctcggcc gtgaacccga
     1981 atttggtagc aagcaggttg tacttgtaag ttccgggttg accttcggtg gccatggtga
     2041 aagccgggaa gcctgctccg ccaaggattt cgtcggtaac gaactgcctg ttgatcagga
     2101 agttcatggc gaatctgacc tctctgatgg caaaggcgtt gaaaacttcc ttgccggcta
     2161 cttcaagagt gtacggcgct tcattgggta cgttgttcat cagaagcgac catgaaccgg
     2221 acggtacagt gtagagatcc agcttgtctc tagtcgcctg gtcgagaccg aagataaccg
     2281 gacccgggac cccatacatg aagatgtctg tcatcccttc tgatgtgtcc ttcagcgcga
     2341 tttcctcttt cattctcacg ttgaagtaaa cgatgtctga aatcggacct ttctccacgg
     2401 caaaagcaaa agatgtcaga accatggata ctaacagtaa gactaacgtt aacctcttac
     2461 tcaaggtact tcgccccctt tgtttcgaga tttttctggt aaatatacca gagttttcct
     2521 tccttattgg tgtggacaaa aagataagaa tgaaaagata taacggtttt tgctacaaga
     2581 ctgcttacag agtaaccatt ccctttcaat tgtcaaacta gttggtacct gcatattatt
     2641 atataccatt caacccaaaa acctagatag gggtaggata tgaatgcgct gttggttgtt
     2701 ggcggtacgt tattggtaag agccgagaaa gagcgtccag agttcgcttg agaaccgttg
     2761 agagtggagc atggagagtt atgggaaaga tcgagacgct cgcttcgctc acgagaaggc
     2821 ga
//
LOCUS       DAOOLY010000294         2820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_43612_length_2820_cov_3.505967, whole genome shotgun sequence.
ACCESSION   DAOOLY010000294 DAOOLY010000000
VERSION     DAOOLY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43612_length_2820_cov_3.505967"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..349)
                     /locus_tag="PLD15_11255"
     CDS             complement(20..349)
                     /locus_tag="PLD15_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB64533.1"
                     /translation="MFQIAIVSGKGGTGKTTLAGSLSFLFDNHAMADCDVDAPNLHLL
                     METELISSFEYYGGKKAVISNDCISCGVCEKYCRFDALIRGGPYRVDPYACEGCGACI
                     VVCPARQ"
     gene            complement(337..678)
                     /locus_tag="PLD15_11260"
     CDS             complement(337..678)
                     /locus_tag="PLD15_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64534.1"
                     /translation="MVETLRKVDYALMVTEPTPFGLHDLKLALQIVREMKIPFGVVIN
                     RDGGTYSMIDEFAREEKIEILEKIPFEREIAKAYSNGVLFTDVLPEWKERFERLYEKI
                     LKSLGVVRCSR"
     gene            complement(711..1199)
                     /locus_tag="PLD15_11265"
     CDS             complement(711..1199)
                     /locus_tag="PLD15_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB64535.1"
                     /translation="MVIAVISGKGGTGKTTLATNFAEVLSSTRKVQLLDADAEEPDVH
                     LFFQISKDYEEEVSLMLPVIDKEKCTLCGECARRCQFGALSVFNTGVMVFNNLCHGCG
                     LCTNICPEGAIGETPKTIGRVELGRISDNLYYGMGILEIGEPSPVRIIRALRSTSTRI
                     RS"
     gene            complement(1429..1719)
                     /locus_tag="PLD15_11270"
     CDS             complement(1429..1719)
                     /locus_tag="PLD15_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HPB64536.1"
                     /translation="MPYGLGYGNRFRDGSCRSFSYGPRGGGRGGRRPGFARGMGPYGR
                     EFYHDYPVAPEIERVDLQEYRSYLNDELELIKMEIQEIEKRLEDLEPQSKGE"
     gene            complement(2046..2327)
                     /locus_tag="PLD15_11275"
     CDS             complement(2046..2327)
                     /locus_tag="PLD15_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64537.1"
                     /translation="MVYGFGYENRLREGHCRSFGYGPRGGRREPGFARGSGVYGRRFH
                     HDHPVTPEMRRARLQLRKSYLNFKLELIKQRIQEVEKRLENLEPQSKEG"
     gene            2468..>2820
                     /locus_tag="PLD15_11280"
     CDS             2468..>2820
                     /locus_tag="PLD15_11280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB64538.1"
                     /translation="MSEGKNHIKMFSVIAPFYNIAFRSQLENYRKLLKENSWRLADKI
                     ESAIDIGCGTGAFARALSELGIEVTAVDASDKMVRIARRNLAGTGVGVVRADFFEDLP
                     FEDNSFDLLVASYVL"
BASE COUNT          698 a          737 c          615 g          770 t
ORIGIN      
        1 gatttgtttt ctctcagtgt tattgccttg ccgggcagac gacgatacag gctccacaac
       61 cctcgcaggc gtacggatcg accctgtaag gtcctccgcg aatcagagcg tcgaatcgac
      121 agtatttttc gcaaacaccg cacgagatac agtcgttgga aatcacagct ttcttgcccc
      181 cgtagtattc gaaggaagag atcaattctg tctccatcaa caggtgaagg ttgggggcat
      241 cgacatcgca gtctgccatc gcgtggttat cgaagagaaa agatagagag ccggccaggg
      301 tggtcttccc ggtgcctccc tttccgctca ctatggctat ctggaacacc gcaccacccc
      361 caaggatttt aggatttttt cgtacagtct ctcgaaacgt tccttccatt cgggaagcac
      421 atcggtgaag agcacaccgt tggagtatgc tttcgctatc tcacgctcga aaggtatttt
      481 ctcaagtatc tctatctttt cttcgcgggc gaattcgtcg atcatcgaat aagttccgcc
      541 atctctgttg atgactacac caaagggaat tttcatctcc ctgacaatct gtaacgccag
      601 cttcaaatcg tgcagcccga agggtgtcgg ctctgtcacc atcaaggcgt aatcaacctt
      661 tctcagtgtt tcaaccaccg gacaggacgt tcccggcgga gaatcaagaa tcacgacctt
      721 atccgggtcg atgtgcttct taaggccctg attatcctca ccggtgatgg ttccccgatt
      781 tcaaggatac ccataccgta ataaaggttg tcgcttattc tacccagctc gacccttcct
      841 atggtcttcg gagtttcgcc gatggctcct tcgggacaga tgttggtgca caggccacaa
      901 ccgtgacaga ggttgttgaa taccataact cctgtattaa agaccgataa ggcgccgaac
      961 tggcatcttc tcgcacactc tccacacaac gtgcattttt ctttgtctat caccggaagc
     1021 atcagactga cttcttcctc ataatccttt gagatttgga aaaagagatg aacgtcaggc
     1081 tcttcggcat cggcgtcgag tagttgtact ttgcgagtgg atgagaggac ttcagcaaag
     1141 ttagtggcga gagtggtctt tccagtaccg cccttgccgc ttatgacggc aataaccaaa
     1201 acctaccacc atctcctgag cctgaagctt cttctgtaac cagtacccaa gcctaatcca
     1261 agtctagatc cacggcggaa aaagtgccca cctaaaaaca ctctccctct gtaatcagta
     1321 ttgacgtcca acccgttcaa agagtctttc ggtgcgcagt ttcccgctcc ccaacctgtc
     1381 atagggccct gacccgaagg tccggttcca tctctccatg gcatagtttc actctccttt
     1441 cgactggggt tccagatcct ccagcctttt ctcgatttcc tgaatctcca ttttgattag
     1501 ctcgagctca tcgttcaaat agcttctgta ttcctgaaga tcgacccgtt ctatttcggg
     1561 agcgactgga taatcatgat aaaattccct accatagggt cccattcccc tggcaaaacc
     1621 gggccgtctt ccgcctcttc ctccgccccg gggaccgtag ctgaaagacc tgcacgagcc
     1681 atctctgaag cgattcccgt aacccaagcc atacggcatt caattcacct ccatatatat
     1741 gttatataca tatataatac tacattatat aagttaaagt caacccgtac ttctacaatt
     1801 tttataatca gtttaatcgg tcaagagttt ctatagaatt tgtcccaata aagggaaaac
     1861 gaaaatgggg aatttatcac tgcgtttttt agaagtttaa tccataggtt catagattgt
     1921 ttccgctgcg agtgtggtca gtatcgtatt aactaacaaa aacagttggg gggcggtttg
     1981 cccgcaccca acctgttatc gggcccagag ctttgagccc ggttccatct ttccacggca
     2041 tgtttttacc cttcttttga ctgaggttcc aggttctcca gtctcttttc gacttcctga
     2101 atcctttgct tgatcagttc gagcttgaag ttcagatagc ttttgcgcaa ctgaagtctt
     2161 gcccgtctca tctcgggagt aaccggatga tcgtggtgaa atctcctgcc ataaacacct
     2221 gaccctcttg caaagccggg ttcccttctt ccgccacggg gtccatatcc aaaagacctg
     2281 caatgacctt ccctgaggcg gttctcgtaa ccaaatccat acaccataaa attcacctcc
     2341 atatatatgc ttactacata tatgatacac tataacatat atgaatgtca actccatatt
     2401 ttcttatctc caaaattaac ctgaatcgat cgcgtgtttc tgatagtatc tctttgaggg
     2461 gagaaaaatg agtgaaggaa agaaccacat aaaaatgttt tcagttatcg caccttttta
     2521 taatatagct ttcagatcgc agctggagaa ttacaggaaa cttctaaagg agaactcctg
     2581 gagactggcc gacaagatag agagcgcgat cgacatagga tgcgggacag gagccttcgc
     2641 cagggctttg agcgagttag gtatagaagt aacagccgtg gatgcttcgg ataagatggt
     2701 gagaatagcc aggagaaatc tggccggaac gggggtcggg gttgtgcggg cggacttttt
     2761 cgaggacctc ccttttgaag acaacagttt tgatctcctg gtggcctctt acgtgttaca
//
LOCUS       DAOOLY010000295         2819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_43629_length_2819_cov_4.431621, whole genome shotgun sequence.
ACCESSION   DAOOLY010000295 DAOOLY010000000
VERSION     DAOOLY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43629_length_2819_cov_4.431621"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLD15_11285"
     CDS             <1..250
                     /locus_tag="PLD15_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPB64539.1"
                     /translation="DRLVEKKIVERKRSEKDRRIVMISLAEEYRQPVRKHLNAFAQKM
                     RRVLSTTTEEDLDFILEGLEKLRTILEESQKSEERGQN"
     gene            250..2475
                     /locus_tag="PLD15_11290"
     CDS             250..2475
                     /locus_tag="PLD15_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64540.1"
                     /translation="MLKLIRYLKPYTVFIVVAVALLFVQAMTELALPDYMSNIVNVGI
                     QQGGIEDAIPEVVSKETFDDVSLFMSQEERQQVLSYYDLISKESANYEENLKKYPILE
                     SKDVYVLKSGEIEDRPSLNLILGKALMAYSGVKNGMVGENGSFAPPSGFTIPEGANVL
                     LLLRLMPEAQRLEMLNQVDSMVEVMGKTIVNQSGALAVKEIYEELGMNTQKLQSGYVL
                     RTGFIMVLMTLLSVLCTIMVAFIASKIAAASARTVRRDVFEKVENFSNSEFAQFSTAS
                     LITRTTNDITQIQLVVVLIIRMVFYAPIIGVGGIIRALEKSASMSWIIALAVIILLGM
                     VGIVFAIALPKFKKMQKLVDKLNLVTREHLSGLMVVRAFNTQKFEEERFDAANRDFTR
                     TDLFVNRVMVVLMPAIMFVMNGTMLLIVWVGAHQIEASTMQVGDMMAYIQYSMLIIFA
                     FLMLTMMFIMIPRASVSGARVSEILEVDPQIKDPKNPKKFSQDFKGTVEFKNVCYRFP
                     GAEKNILNSISFTAIPGQTTAIIGSTGSGKSTLLNMIPRFYDVCGGQVLVDGIDIREV
                     SQHDLRERIGYVPQKGVLFSGTIESNLKYADETAPKEEVERAAEIARAVEFITAKEEG
                     YDSPISQGGTNVSGGQKQRLSIARALLKRPQIFLFDDSFSALDFKTDAALRRKLKEET
                     GDSTMIIVGQRIATIKNADQILVLDEGNLVGIGKHHQLMKSCQIYREIALSQLSEAEL
                     A"
     gene            2472..>2819
                     /locus_tag="PLD15_11295"
     CDS             2472..>2819
                     /locus_tag="PLD15_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64541.1"
                     /translation="MNGDNREKERRPQDRGGGPMGPGMMRSGEKAKDFRGTLKKLVQY
                     MKKYQILILVVFCFAAAGAVFSIVGPKILGGVTTEVFEGIMGKITGTGEGVNFDSVAR
                     TMLILLGLYVLSAL"
BASE COUNT          807 a          692 c          746 g          574 t
ORIGIN      
        1 cgacagactc gtcgaaaaga agatcgttga aagaaagaga agcgaaaaag acaggcgaat
       61 cgttatgata agccttgccg aagagtatcg ccaacctgta aggaaacacc tcaatgcctt
      121 tgcccagaag atgagaaggg tattgtctac caccacagaa gaagatcttg attttattct
      181 ggaagggctt gaaaagctga ggacaatact cgaagaatca cagaaatccg aagaaagagg
      241 gcaaaactga tgctcaaact gatcaggtac cttaagccct acactgtatt catcgtggtc
      301 gcggtggcac tgctcttcgt ccaggccatg acggagctcg ccctgcccga ttatatgtcg
      361 aatatcgtaa acgtcggtat tcagcagggc ggcatcgaag acgccatacc ggaggtcgta
      421 agcaaagaaa cgttcgatga cgtgtcactg ttcatgagtc aagaagagag gcaacaggtc
      481 ctgagctact acgatctcat aagcaaagaa tcggccaatt acgaagaaaa cctcaagaag
      541 taccccatat tggagagcaa agatgtctac gtcctgaaat ctggagagat tgaagatcgc
      601 ccaagtctta atttaatcct tggcaaggcg ctcatggcct attccggagt caagaacggc
      661 atggtcgggg aaaacgggag ctttgcgccc cccagcggtt tcaccattcc ggaaggggca
      721 aacgtgctct tgctgctacg cctaatgccg gaagcccaga gactcgaaat gctgaatcaa
      781 gttgatagca tggtcgaagt catgggcaag accatcgtca accagtcggg cgcccttgcc
      841 gtgaaggaaa tctacgaaga gcttggcatg aacacccaga agctgcagag cggctacgtg
      901 ctacgcaccg gttttataat ggttctcatg accctgctga gcgtactgtg caccataatg
      961 gtcgccttca tcgcttcgaa gatagcggcc gcctcggcca gaacagtgag aagagatgtc
     1021 ttcgaaaagg tcgaaaactt ctcgaactct gaattcgccc agttctctac ggcctcgctc
     1081 attaccagaa cgacgaacga cataactcag atacagctcg tggttgttct cataataaga
     1141 atggtattct acgcgccaat cataggtgtc ggcggaatca taagggcact cgagaagagc
     1201 gcctccatgt cctggatcat cgccctcgcc gtaatcatac tactcggcat ggtggggatc
     1261 gtgttcgcca tagcccttcc gaaattcaag aagatgcaga aactcgtaga caaactgaac
     1321 ctggttacaa gggaacacct gtcaggcctc atggtcgtca gggccttcaa cacccagaag
     1381 tttgaagaag agagattcga cgccgcgaac agagacttta caagaaccga cctcttcgtc
     1441 aaccgggtaa tggtcgtact gatgccggcg ataatgttcg tcatgaacgg aactatgctc
     1501 ctcatcgtct gggtgggggc acaccagata gaggcctcaa ccatgcaggt tggagacatg
     1561 atggcctaca ttcagtactc catgttgatt atcttcgcct ttctcatgtt gacaatgatg
     1621 ttcatcatga tcccgagggc ctctgtttcc ggagcgagag tgtcagaaat cctggaagtc
     1681 gatccccaga taaaagatcc caaaaatccg aagaaattca gccaggattt taaaggaact
     1741 gttgaattca agaacgtctg ctaccgcttc cccggtgccg agaaaaacat cctgaacagc
     1801 ataagcttca cggccatccc ggggcagacg acggcgatca tcggctctac cggttcgggg
     1861 aaatcgacgc tgctgaacat gataccgagg ttctacgatg tctgcggcgg ccaggtcctg
     1921 gttgacggaa tagacatccg ggaagtgagc cagcacgacc tgcgggagcg cataggctac
     1981 gtcccccaga agggcgtcct cttcagcgga accatagaat cgaacctgaa atacgccgat
     2041 gaaacggctc ctaaggaaga agttgaaagg gctgccgaaa tcgcccgggc cgtcgaattc
     2101 ataaccgcga aagaagaagg gtatgactcc ccaatttccc agggcgggac aaatgtatcg
     2161 ggcggacaga agcagaggct ttccatagcc agagcgttgc tgaaaaggcc ccagatattc
     2221 ctcttcgacg acagcttctc tgccctggac ttcaagacag acgcagcgct gagaagaaaa
     2281 ttgaaagaag aaactggaga tagcacgatg ataatagtcg gccagagaat tgcgacgata
     2341 aagaacgccg atcagatact cgttctggat gagggcaacc ttgtaggaat cggcaaacac
     2401 caccaattga tgaaaagctg tcagatatac agagaaatcg cactgtcaca gctttcagag
     2461 gcggaattag catgaacggg gataacagag aaaaggaaag aagacctcaa gatcgtggtg
     2521 gcggtccgat gggcccaggc atgatgcgct cgggagagaa ggccaaagac ttcagaggaa
     2581 cactaaagaa actggttcaa tacatgaaga agtaccagat actgatactc gtagttttct
     2641 gtttcgcggc ggccggtgcc gtgttttcca tcgtagggcc taagatactc ggaggggtga
     2701 cgaccgaggt cttcgaaggc atcatgggca aaatcaccgg taccggcgaa ggggtaaact
     2761 tcgactccgt cgcaaggacc atgttgatcc ttctcggact gtatgtgctc agcgcactg
//
LOCUS       DAOOLY010000296         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_43712_length_2815_cov_3.829348, whole genome shotgun sequence.
ACCESSION   DAOOLY010000296 DAOOLY010000000
VERSION     DAOOLY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43712_length_2815_cov_3.829348"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLD15_11300"
     CDS             <1..547
                     /locus_tag="PLD15_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB64542.1"
                     /translation="PDIIMVGEIRDRETAQLAIEASLTGHLVFSTIHTNSASGAVARL
                     VNMGVDPFLLGTSLIGVVGQRLVRKLCNNCKIKIPMREEVKRVASHIYPNRDDFIEYI
                     PGNGCPECRGMGYRGRTGIHEVLIVDNKLRQLIAENASEREISAAATSGGMRTLYTDG
                     VQKVIDGVTSIEEIKRVAIEI"
     gene            713..1738
                     /gene="ord"
                     /locus_tag="PLD15_11305"
     CDS             713..1738
                     /gene="ord"
                     /locus_tag="PLD15_11305"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HPB64543.1"
                     /translation="MAYRVLVWGLGAMGSGIAKNVLRKKELRLVGAIERDPSKIDRDL
                     GEVLGLERNSRRVYSDPEKAILETRPDIVLIATNSFVDEVMPKIELTAKNHVNVLTIA
                     EEMAYPYKSNPEESEILENLAWKYGISILGTGINPGFVLDLLIIAMTGACLRVDRIKA
                     SRINDLSPFGKTVMETQGVGTTPEEFKRGLEEGDIVGHIGFQQSIAMIGNAMGWKIDR
                     IEETREPIISATERKTAVAHIKPGIVAGCRHTGRGFCGGELKIELIHPQQILPAIEGI
                     ETGDYIDIYGEPDIHLSIKPEIPGGKGTIALATNMIPEVINAAPGLLDMSELPVPKCL
                     MDDIKES"
     gene            1741..2052
                     /gene="ortA"
                     /locus_tag="PLD15_11310"
     CDS             1741..2052
                     /gene="ortA"
                     /locus_tag="PLD15_11310"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HPB64544.1"
                     /translation="MMARRGQWVQIHSILLKAGERAPSVPSDTADVPLELRVRGFLQD
                     GSASIGQVVRILTAAGRLVEGALEIIEPRHVHDFGDYVPELSQAGDELTIWLAGGDEN
                     E"
     gene            2045..>2815
                     /locus_tag="PLD15_11315"
     CDS             2045..>2815
                     /locus_tag="PLD15_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HPB64545.1"
                     /translation="MSDMSYESVMSRKAQIMRASIGIEYDKYELEGIGFDYEGLMSDT
                     AYPMEEITAIQRETGVGNTPLVELKNITRLVREISEPGKGARIFLKDEAANPSGSFKD
                     RRASVSVARAKELGYRGVIAATSGNYGAAVASQAMKRGLKCIVIQECYDRTGTGQPEI
                     LEKARACEAYGAEVAQLSVGPELFYYFLLLLEETGYFNASLYTPFAIAGIESLGFEIA
                     QQSRRVIGKDPNFVLATHAGGGNLTGTARGLIKAGAEKT"
BASE COUNT          806 a          662 c          788 g          559 t
ORIGIN      
        1 tccggacatc ataatggtag gagagatccg tgacagagaa acggcccagc tggccatcga
       61 agcttcgctc accggacacc ttgtcttctc gacgatacat accaacagcg cctccggagc
      121 cgtggctaga ctcgtgaaca tgggtgtaga tcccttcctg ttgggaacct cgttaatagg
      181 tgttgttggt cagagactgg tgagaaaact ttgcaacaac tgcaaaatca agatcccgat
      241 gagagaggaa gtaaagagag tggcaagcca catctacccc aacagggacg atttcatcga
      301 atacatccct ggaaacggct gccccgaatg ccgcggaatg ggttaccgtg gaagaaccgg
      361 tatccacgaa gttctgatcg tggataacaa gttaagacag ctcatagccg aaaacgcatc
      421 ggaaagagag atctccgctg cggcaacttc cggaggaatg agaacgctct acaccgatgg
      481 agtccagaaa gtgatagacg gagtgacatc gattgaggag ataaagagag tcgccataga
      541 aatctgattc ggttacactc catactcacg catacccaaa catactcgaa caagattaga
      601 atattcggct tttatcgccg atagccaatg attgcccaaa tagcctagcc ccggtttcga
      661 tggtaggatt taactggaga gatacagaga aaatcgaaca gggaggttgt ttatggcata
      721 cagagtgctt gtatggggtc ttggtgccat gggaagcggc atcgcgaaaa acgttctaag
      781 aaagaaagaa ctacggctgg ttggtgccat cgagagagat ccgtcgaaga tcgacaggga
      841 tctcggtgaa gttctcggtt tggaaagaaa cagtcgcaga gtttactccg atccggaaaa
      901 ggcgattctg gagacgagac cagacatagt tctaattgcg acgaattcct ttgttgacga
      961 agtcatgccg aaaatagaac tcactgcgaa gaaccacgta aacgtactta ccatcgcaga
     1021 ggaaatggcc tatccgtaca agtccaaccc cgaagaatct gaaatcctgg aaaacctcgc
     1081 atggaaatac ggaatctcca tcctcggcac gggaatcaac ccgggttttg tactggatct
     1141 gctgataata gccatgacag gcgcctgtct aagggtcgat agaataaagg ccagcaggat
     1201 aaacgacctc tcgccttttg gcaagaccgt catggagacc cagggcgtgg gaaccactcc
     1261 agaggaattc aagcgtggtc tagaagaggg agatatagtc ggtcacatag gctttcagca
     1321 atcgatagct atgatcggca acgccatggg ctggaagatc gacagaatcg aagaaaccag
     1381 ggaaccaata atctcggcta cggaacgaaa aacagcagta gcccacataa agcccggtat
     1441 agtggccggt tgtaggcaca cgggcagagg tttctgcgga ggcgaattga agatagagct
     1501 catacacccg caacagatac tgcccgcaat agagggcatc gagaccggcg actacataga
     1561 tatctatggt gaacccgata tacacctttc aataaaaccg gagatccccg gcggaaaagg
     1621 cactatagcg ctggcgacca acatgatacc ggaagtcata aacgcagcac ctggattact
     1681 ggacatgagc gaattgcccg ttccaaaatg cctgatggac gacatcaagg agagctagat
     1741 atgatggcca gaagaggaca gtgggtgcag attcacagca tcctgttgaa agccggggag
     1801 cgtgccccat cagttccctc cgacaccgca gacgttccac tggaattgag ggtcaggggt
     1861 tttctacagg acggatcggc ctccatcggc caggtcgtca ggattctaac ggccgccggc
     1921 aggctggtag aaggagctct cgagataata gaaccccgac acgtgcacga tttcggtgat
     1981 tatgtccccg agctttcgca ggccggtgac gagttgacca tctggctcgc cggtggtgat
     2041 gaaaatgagt gatatgagtt atgaatcggt gatgtcgaga aaggcacaaa tcatgcgggc
     2101 ttcgataggg atcgaatacg ataagtatga acttgaaggt atcgggtttg actacgaagg
     2161 cttgatgagt gacacagctt accctatgga agaaataacg gccatccagc gtgagacagg
     2221 tgtcggaaac acacctctgg ttgagcttaa gaatatcacg agactggtca gagaaatcag
     2281 cgagccaggc aaaggggcca ggatctttct caaggacgag gctgccaacc cctcaggaag
     2341 tttcaaagac aggagggctt cggtcagcgt tgccagggct aaggagctcg gttacagagg
     2401 tgtcatcgcg gcaaccagcg gcaactacgg agcggctgtt gcctcgcagg cgatgaaaag
     2461 aggtctcaaa tgcatagtga ttcaggagtg ttacgacaga actggtactg gtcaacccga
     2521 gatactggaa aaagcgagag cctgcgaggc ctacggcgcg gaggttgcgc agctttcggt
     2581 gggacctgaa ctcttttatt actttttgct cctgcttgaa gagactggct acttcaacgc
     2641 ctcgctatac acgccatttg cgatagccgg aatcgagtcg ctcggcttcg agatagcaca
     2701 gcagtcgaga agagtgatcg gtaaagatcc caatttcgtt ctagcgaccc acgcaggcgg
     2761 aggtaacctc accggaacgg ccagggggtt gattaaggct ggagccgaaa agacc
//
LOCUS       DAOOLY010000297         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_43837_length_2810_cov_4.558984, whole genome shotgun sequence.
ACCESSION   DAOOLY010000297 DAOOLY010000000
VERSION     DAOOLY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43837_length_2810_cov_4.558984"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2810
                     /locus_tag="PLD15_11320"
     CDS             <1..>2810
                     /locus_tag="PLD15_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretory pathway, component HofQ"
                     /protein_id="HPB64546.1"
                     /translation="VVDEYSLEQISEKLIYIYAPSDILDEALVELENREKQIFRKTED
                     LILEVAPEIYDSGLGELIDTLLNPEESQVFIRSAGLIVLRGYPERLSELEKIIEARIP
                     RIVEMIEKRLSGQETRMSLQIGTIPGWDLDKFQTYLRDFLGPDEFATITVTRSGNGYI
                     VVGPENLLNTVDDETRKLRELENPFYRLESSIPSLSQFELLLERLGVAVSIMPVDNQF
                     MVIGSQENVEEASRLIRQLGSGLPVGGEVTVPGTLYNYAFVNVPYGEVAGLSELLEKL
                     NLPVEFVNTPTGVAAVAPEERLKKAMEVVESILSRLEPVEAGPVGYSLVDVDPGSLES
                     LKTIIEKLRIEVDFVDTPSGIVGIGSDAHLDRVKKLIEDLLKKESMTPPQGDKETGYM
                     IFRGRSDVSVDSLKSVVQVFGYDLTFTSVYDKIVAVGDRYDLNSFNTLYGEILSSESV
                     EDTRVSVELPGIPGWDTTRLSAYLGVVFGEKAEEISLVQTSKGYILNAPGSMASAIGE
                     EFERLSRIENPSYSIETRMPNITEIDSLLARLGIIVDILQVEDYSVVVGSSENVAKAR
                     ELITKLVDTLGVPEQTKPQVFKLSAITSGDMEYFVPIFGSIDIEVALLESPAGILIVG
                     KEDQVNRALEIADSILERKPSQEVSTAYEFVDIPSDGVTTYGEILKRLGLSAELFPSP
                     SGVLLIGSEEDIDKARPIIEAILEREAKTVSQSDEQQRVSMIVKNIPGWTEDKFTGYF
                     RSLLGESEFAGISITPSVSGFIVIGEEKALAKMQNEVVRLSEIEDPFYLVKDTLPPVD
                     QLDRLLERLGLNVSILPVGEKNLIVGVESDVIKTGELIDTLKTDLDTGDEKYVYRFSD
                     VNPQLKGVLESILSSLKIEVGLTEAGNSMAIVGLEKEVDAAKVILDDVAGQIVGDTAK
                     GKIGYSFVGVESSQIDSYSEIL"
BASE COUNT          785 a          628 c          756 g          641 t
ORIGIN      
        1 ggttgttgac gagtattctc tggagcagat atccgaaaaa ctcatttata tctatgctcc
       61 ctcggatata ctcgatgaag ccctggtgga actggagaac agagagaaac agatattcag
      121 aaaaaccgaa gatcttatac tcgaagtggc tccggagatc tatgactcgg gcctgggaga
      181 gctgatagat acactcctga atcccgagga atcgcaggtc ttcatcagat cggccggatt
      241 gatagttctc aggggttatc cggaaagact gtcggaattg gagaaaatca tagaggcgag
      301 aattcccagg atcgttgaga tgatcgagaa gcgtctctcc ggacaggaaa ccaggatgtc
      361 tctccagata ggcacgatac ccggctggga tctcgataaa ttccaaacat atctaagaga
      421 ctttctgggg ccggatgaat ttgccaccat cacagttacg agatccggga acggatatat
      481 agtcgtaggt ccggaaaatc ttttgaacac agtggatgat gagaccagaa agctcagaga
      541 gcttgaaaat cccttttaca ggcttgagtc gtcgataccg tctttgagcc aattcgaatt
      601 gctactcgag agactcggtg ttgccgtatc tataatgccc gtagataacc agttcatggt
      661 gatcggatct caggaaaatg tagaagaagc ctcccgtctt ataagacagc ttggcagcgg
      721 tttgccggtc ggaggagaag tgacggtccc cggcaccctt tacaactatg ccttcgtaaa
      781 cgtcccttat ggagaagtag ccggtctatc agaacttctc gagaaattga acctacccgt
      841 ggagttcgtg aacacaccaa ccggagttgc cgcggtcgct cctgaagaga ggttgaagaa
      901 ggccatggaa gtggtggaat ccatcctctc aaggcttgaa cctgttgaag ccggacctgt
      961 tggttacagc ctcgtggatg tagatcccgg ctctctcgaa agcctcaaaa cgattatcga
     1021 gaaattgagg atcgaagtgg atttcgttga taccccttcc ggaatcgttg gtattggaag
     1081 cgacgcccat ctggacaggg tgaaaaaact gatagaggat cttcttaaga aagaatcgat
     1141 gactcctcct cagggcgata aagagacggg gtacatgata ttccgcggca gaagcgacgt
     1201 cagcgttgat tcgctcaaat ccgtcgtgca ggtcttcgga tacgatctta ccttcacttc
     1261 agtttacgac aagatcgttg cggtcggaga caggtacgac ctgaacagct tcaacacgct
     1321 ttacggagag atactctcca gcgaaagcgt tgaagatacc agagttagcg tagagctacc
     1381 cggtatcccc ggctgggata ctacaagact ctccgcttac ctgggcgttg tatttggcga
     1441 aaaggccgaa gagatctctc tggtccagac ttcaaaggga tatatattga acgctcccgg
     1501 atcgatggca tctgccatcg gtgaagagtt tgaaagactt tcaaggatcg agaatccctc
     1561 ttattccata gagacgagaa tgccaaacat aacggagata gatagcctcc tggcgagact
     1621 cggaatcatc gtcgatatac tccaggtaga agactacagc gtcgtggtcg gttcttcgga
     1681 gaacgttgcc aaagcgcgcg agttgataac taaactggtt gacactctgg gagttccaga
     1741 gcagacaaag ccccaggttt tcaaattatc cgccataact tccggagata tggagtactt
     1801 cgtacctatt ttcgggagta tagatatcga ggttgcgctc cttgaatctc ctgcaggaat
     1861 attgatcgtc ggcaaggaag accaggtgaa cagggcgctc gaaatagccg acagtatcct
     1921 ggaaaggaaa ccctcacaag aagtttcgac agcgtacgag ttcgtcgata taccttcaga
     1981 tggtgttacc acttacggcg agattctgaa gcggctgggt ttgagtgccg aactctttcc
     2041 ctcaccatcg ggtgttcttc ttataggatc ggaagaagat atagataaag ccaggccaat
     2101 aatcgaggcc atactggaac gtgaagcgaa aaccgtttcc cagagcgatg aacagcaaag
     2161 agtatcgatg atcgtcaaaa atataccggg ctggacggag gacaaattca ccggttactt
     2221 cagaagcctc cttggggaga gcgagtttgc cggaatctcc ataaccccct ctgttagcgg
     2281 ctttatagtc ataggagaag agaaagctct ggcgaagatg cagaacgaag tcgtcagact
     2341 gtcggagata gaggacccct tctatctggt aaaggatact cttccaccgg ttgatcagct
     2401 cgacaggctc ttggagagac tcgggttgaa cgtgagcatc ctgccggttg gggagaaaaa
     2461 tctgatcgtc ggcgtggaaa gcgacgtaat taagaccgga gagttgatcg atacactcaa
     2521 gacagatctg gatacaggcg atgagaagta cgtttacaga ttttccgatg tcaatccaca
     2581 acttaagggc gttttagaaa gcatcctctc cagtctgaag atcgaagtgg gactgacaga
     2641 agcgggaaac agcatggcga tcgttggcct ggagaaagaa gttgatgccg caaaagtaat
     2701 cctggacgat gtagcaggac agatagttgg cgatacggcg aagggtaaaa tcggatactc
     2761 cttcgtggga gtcgagagct cgcagataga cagctacagc gagatactgg
//
LOCUS       DAOOLY010000298         2781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_44488_length_2781_cov_3.840426, whole genome shotgun sequence.
ACCESSION   DAOOLY010000298 DAOOLY010000000
VERSION     DAOOLY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44488_length_2781_cov_3.840426"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLD15_11325"
     CDS             complement(<1..646)
                     /locus_tag="PLD15_11325"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPB64547.1"
                     /translation="MYRTACTVLFVISAFFCGLAVSNYIDPAGYPYPWPDNPSPALAS
                     YEFNSLSDLTDYYPTSSWSVSGGILSITGSGERRAILNGNTSWNNYIVKSYGLITGGI
                     TSGGSGYAIYVRVTGTARNINAYAFQVDPGLGNMFVLRKVINGAESGVLASGTPAAGF
                     DWLQWHEVTVVANGNTFIMYVDGIEVLRYTDNSNPLMNGRVGLRSWGPTQVKFDY"
     gene            complement(1191..2399)
                     /locus_tag="PLD15_11330"
     CDS             complement(1191..2399)
                     /locus_tag="PLD15_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPB64548.1"
                     /translation="MRVLVTGARMWYALNSIRLLARQGHEIFAADSGKVSGGLYSKYL
                     KGKFIYPPVSEKSDEFIECLLKKIEELKIDLLFPTFEEGFVISRFMHKFEGRVRVIVP
                     RHEHIQVLHDKWTMTQLASSLSIPVPDTTLLKDFDTSRMDFPLIVKPRDQRSAEGIVK
                     VQTLSQLKKLSATLDGTKTLVQEWKTPHQICTTGLALDGKLKGNVIYHNIREYPESGG
                     VGTCRVSIKCEKVESYVSTIVEALRYSGFISMDFLYDRNEDEYYLVDVNPRMSPGLLV
                     AHTSGMDFVRGYMDMVDGDIKSFDVVVPGRGTYTTAMEIGWYFSTLFKGKFRNLKGFF
                     KSRKGLKDDTWDIRDPLPFFVVLFAMAYTAVFGPFMGGQTKGFSLGATYDSEKFSVEK
                     AESEKNREAG"
BASE COUNT          715 a          682 c          616 g          768 t
ORIGIN      
        1 catagtcgaa tttgacttgg gttggacccc atgacctgag acctacgcgg ccgttcatta
       61 aaggattact gttatcggtg taccttaaaa cctcaattcc atcgacatac ataataaaag
      121 tgttgccgtt ggctactacc gtcacttcat gccactgaag ccagtcgaat ccagccgctg
      181 gagttcccga agccagaacg cctgactcgg ctccattgat tacctttcta agaacgaaca
      241 tgttgcccag acctggatct acctgaaagg catacgcgtt gatgtttctt gcagtgcccg
      301 tgactctcac atatatcgca taaccagagc cacccgatgt gattcctccg gtgatcagcc
      361 catagctttt tacaatgtaa ttgttccagg aagtgtttcc gtttagaata gctctgcgct
      421 cacctgagcc ggttatcgag aggatgcctc cggaaacgct ccaggaactt gtcgggtagt
      481 aatctgtgag atctgaaagg ctgttgaatt catagctggc cagggcagga ctgggattat
      541 cgggccatgg ataagggtat ccggccggat cgatgtagtt ggagacagcc aatccgcaaa
      601 aaaacgctga gataacaaaa agcacagtac acgctgtacg gtacatgatt ttgccccctg
      661 ttgtatattg taccttctat atctctcaaa aaacgataag tgagacatat taacttagaa
      721 aatgggataa tttttattca ttcaaattat acataataat ttttcatacc gcaagcggtt
      781 cgaagacacc agcactgaaa gctttatctc gaggcgattt cagcagttat tatcatcgcg
      841 ttcctcggac cgctcaatcc cgcgaatcca aaacaagcgg ttacttagtg aggccggtgg
      901 gcgaacctgt atcgccgaaa gctccggaag ggttgtatct tcttctgtga aacttgatcg
      961 attgtttgga cctttccccg atatgtaagt tcggctgtta catctaatgt attccatccg
     1021 tgtgcgaaag acatttcagg gcgtttttct ctataaggct ctattaaaca acttgataaa
     1081 gcttgacatc gatactgtcg gatatggtat aattatacat gagttaaccc tcagaccgct
     1141 tcgctgtcct tgaaagggca gcgctttttt attaaacaac cgacttataa ttaaccggct
     1201 tctctgtttt tctcggattc ggccttttcg actgagaact tctccgaatc gtaagttgcg
     1261 cccagcgaga aacccttcgt ctgccctccc ataaacgggc cgaagacggc cgtataagcc
     1321 atcgcaaaga gtacgacgaa gaaggggagc gggtccctta tatcccacgt gtcgtccttc
     1381 aaccctttcc tgctcttgaa gaagcccttc aggttcctga actttccctt gaacagagtg
     1441 ctgaaatacc agccgatctc catggcggtc gtgtacgttc ccctgcccgg aacaaccacg
     1501 tcgaagcttt ttatatcgcc atcgaccatg tccatataac ctctgacgaa atccatcccc
     1561 gaggtgtgcg cgaccagaag ccccggactc attctggggt tgacgtcaac gagatagtac
     1621 tcgtcttcgt tccggtcgta gagaaaatcc atcgaaatga atcccgagta tctcagggcc
     1681 tccactatcg tggaaacgta agactccacc ttttcgcatt ttattgatac cctgcaggtt
     1741 ccgaccccgc cggactccgg gtactccctt atattgtgat agatgacgtt tcctttaagt
     1801 ttgccgtcta aagccagccc tgtcgtgcat atctggtggg gcgttttcca ttcctggacc
     1861 agtgtttttg tgccgtccag agtggcggag agctttttca gttgcgaaag cgtctgaact
     1921 tttacgatcc cttcggccga cctctggtcg cgtggcttca cgataagcgg gaagtccatc
     1981 ctggaagtgt cgaagtcctt tagaagcgtt gtatcgggaa cgggaatcga gagagaggag
     2041 gccagctgcg tcatggtcca cttatcgtgc aaaacctgta tatgttcatg cctgggcacg
     2101 ataaccctga cacgcccctc gaatttgtgc atgaatctgc ttataacgaa gccttcttcg
     2161 aaggtgggga aaagtaagtc tatcttcagc tcttctattt ttttgagaag acactctatg
     2221 aactcgtcgc tcttctcgct cacgggagga tatatgaatt tacctttcag atatttcgag
     2281 tacagaccac cgctgacctt gccgctgtcg gcagcgaaga tctcatgtcc ctgtctggcc
     2341 agaagccgta tactgttgag cgcataccac attctggcgc ctgtaaccag taccctcatc
     2401 gaacaccccc gaaatctatt ctatagtaaa ttagggatag tagaaatctg ttaacatatc
     2461 aacatccgat ctgtgtaacg atcccaaatt taattatatc atctccggtg gaattggaac
     2521 aaattgcagg cttggatcga tgctttgttg gccgattttg agatatggaa agaaaataat
     2581 agagcagtat tccatataca atttacacat taatttacat aaacgaggat atttgacttt
     2641 tagggaatta attgtataat atatctgcaa ggttgttggt taacttttga catacaaata
     2701 cgatagggac ccgataaaaa ggaaatgaag ttagccgcaa tccggacaac tcctcgtttc
     2761 gtagcaattc ccccttcgat t
//
LOCUS       DAOOLY010000299         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_45031_length_2756_cov_4.202518, whole genome shotgun sequence.
ACCESSION   DAOOLY010000299 DAOOLY010000000
VERSION     DAOOLY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45031_length_2756_cov_4.202518"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1121)
                     /gene="wecB"
                     /locus_tag="PLD15_11335"
     CDS             complement(12..1121)
                     /gene="wecB"
                     /locus_tag="PLD15_11335"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPB64549.1"
                     /translation="MKKVALIFGTRPEAVKMAPVYLAMKDSKLEPIVIATAQHRQMLD
                     QVLGLFGISPDFDLDIMQERQSLSGLTSRLIQKLDEIYAANSFDATLVQGDTTSTFAG
                     ALVSFYHKIPVGHVEAGLRTDNIYNPFPEEVNRRLNGVLSTYHYPPTESAKGNLLHEG
                     VPPDRMVVTGNTVIDALLWTIENKAGATSSFREKFGLENGRYILMTMHRRENWGEPMR
                     NVMLAVRDILDSMPEMKLVFPVHLNPVVRETVYPLLKGHERVILTDPLDYLPFVSLME
                     KAALILTDSGGIQEEAPALGKPTLVLRETTERPEAIQAGTALLVGTDRKKVFEETVRL
                     LTDRSAYERMAMASNPFGDGKASLRIAEHLERVLA"
     gene            1393..>2756
                     /gene="murJ"
                     /locus_tag="PLD15_11340"
     CDS             1393..>2756
                     /gene="murJ"
                     /locus_tag="PLD15_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPB64550.1"
                     /translation="MTASTVRNTAIFAIATLLSRVSGLVRDSMFAGYFGTSRQYDAYL
                     VAVMLPFFLRKIFADGAMTMAFVPIFNDRLGRSRERAFEFASAVMIFVLLLSGLISAG
                     GVFFSRELTGIFAGGFSGDTIELTSQLIKISFPFITLVSLWAVYCGILNSLDAFFIAA
                     ASPMLINLATIVGILLSEKFTPPILGPTIGFTAGGAIQLLAVMLAARRSGFRFNPRYN
                     SHDARDFLSLFLISSISPAINEINSFVDTRVSTGLGTGAVSSLGYAQRLYQLPLGIFA
                     IAVATVALPQLSRLTGKDSKEEFSKAVWSSITVLAFLLLPSTLGLLVAGEGLVKLLFE
                     RGSFTALDTAVTSGLLYAYTLGLPFYGAYGVLSRAYYARKSPVTPTIISALMVGVNVV
                     LDIVLGFTMGPVGVALATSIAGMLGTTIVTVTLFAWAGYERKALVSIGKIMLASLIMT
                     AAMLV"
BASE COUNT          544 a          754 c          746 g          712 t
ORIGIN      
        1 ataatcgctg atcaggcgag tacacgctcc agatgctcgg cgatcctcag agaggccttt
       61 ccgtcgccga agggattgct tgccatcgcc atcctctcgt aggccgaccg gtcggtcagc
      121 aaccttaccg tctcctcaaa gacctttttc ctgtcggtgc cgactagaag ggccgtgcca
      181 gcctggatgg cctcgggtcg ctccgtagtt tccctgagaa ccagcgtggg ctttccgagc
      241 gccggtgcct cctcctgtat accaccggaa tcagtgagta tcagagcggc cttttccatt
      301 agagagacga agggcaggta gtcgagcgga tcggtgagaa tcaccctttc atgtcctttg
      361 agcaaaggat agaccgtttc cctcacgact ggatttagat ggaccgggaa aaccagtttc
      421 atctccggca tggaatcgag tatatcccta accgcgagca tcacgtttct catcggctct
      481 ccccagttct cccttctgtg cattgtcatc agtatatacc ggccgttttc gagaccgaac
      541 ttctccctga aagaggaagt ggctccggct ttgttctcga tagtccacag cagggcatct
      601 atgaccgtgt tccccgtcac taccattcta tcggggggaa cgccttcatg gagcagatta
      661 cctttggcgc tttcggtggg aggatagtga taggtagaga gaactccgtt cagtctgcgg
      721 ttgacttcct cggggaaggg gttgtagatg ttgtcggtcc tgagtccggc ctccacgtga
      781 ccaaccggaa ttttatggta gaaggagacg agtgcgcctg caaacgtgga cgtcgtatcg
      841 ccctggacca gggtggcatc gaaggagttg gccgcgtaaa tctcgtcgag cttctggatc
      901 aatctcgaag tcagtcccga cagggactga cgttcctgca ttatgtctag atcgaagtcg
      961 gggcttattc cgaagagccc gagtacctga tcgagcatct ggcggtgctg tgcagtcgcg
     1021 atcactatcg gttcgagttt cgagtctttc atggcgagat agaccggggc catcttcact
     1081 gcctccggtc tggttccaaa tatcagcgca acttttttca ttggcttcat acgccgccgg
     1141 ggatcatacg gctactctcc tttccgcggt gttggccggt tacaggtgcg accaactctt
     1201 tcatgcacag agtatagcac agatccaggg gcgattcttt aagggttgac cctgcaaaag
     1261 acgggaggga tcggagtgtt cttttcgaga gcacgaagtg aagtcttata actcccattt
     1321 tagttacatc gattcaaagg atctctcagt cgaaaccggc cgcctgtatt ataatcttct
     1381 ttggaggatc aaatgacggc cagcacagtc aggaatacag ctatctttgc catagccaca
     1441 ctgctttcaa gagtctccgg ccttgtgaga gactctatgt ttgcaggcta tttcggcact
     1501 tcccggcagt acgacgccta tctggtcgcc gtgatgttgc ccttcttcct gaggaagatc
     1561 ttcgccgacg gagccatgac gatggccttc gtccccattt tcaacgacag gctcggtcgc
     1621 tcgagagaga gggccttcga gttcgcctcg gccgtaatga tattcgttct gctactgtcc
     1681 ggtttaataa gcgctggcgg cgtatttttt tcgcgagagc tgacgggtat attcgccggc
     1741 gggttcagcg gcgataccat cgagctgaca tctcaactga taaagatctc cttccccttc
     1801 ataactctgg tctccctgtg ggccgtttat tgcggcatac tgaacagtct cgatgccttc
     1861 ttcattgctg ccgcctcgcc catgctgata aatctggcga cgatcgtggg aatactcctc
     1921 tccgagaagt tcactccacc catacttggc cccacgatcg gtttcacggc cggcggagcg
     1981 atccagcttc tggcagttat gctcgcggcc aggcgttccg gcttccgttt caacccccgt
     2041 tacaacagtc acgacgcgcg ggattttctt agtctctttc tcatctcttc gatatctccg
     2101 gctatcaacg agataaactc tttcgtcgac accagagtat ctacggggct cggcaccggc
     2161 gccgtttcca gtctgggtta cgcacagagg ctgtaccagc ttcctcttgg catattcgcg
     2221 attgcagtcg cgacggtggc ccttccacag ctctcaaggt tgacgggaaa ggattctaaa
     2281 gaagagttca gtaaagccgt gtggagctcg ataaccgttc tggccttcct gcttctgccc
     2341 tcaacgctcg ggttactggt ggccggcgag ggactggtga aactactttt cgagaggggc
     2401 tctttcaccg cactggatac ggcggtcact tcgggcctac tgtacgccta tactcttgga
     2461 ctgcctttct acggtgctta cggtgtgctg tcgagggcgt attacgcgag gaagagccct
     2521 gtaactccca ccataatatc cgctctgatg gtaggtgtga acgtagtcct ggatatcgtt
     2581 ctgggcttta cgatgggtcc ggtgggcgtt gcgctggcaa cctctatcgc aggaatgctc
     2641 ggtacgacta tagtcactgt gactcttttc gcctgggcgg gttacgaaag aaaggcgttg
     2701 gtcagtatcg gtaagataat gctggcgtct ctgataatga cggccgcgat gcttgt
//
LOCUS       DAOOLY010000300         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_45614_length_2730_cov_4.820935, whole genome shotgun sequence.
ACCESSION   DAOOLY010000300 DAOOLY010000000
VERSION     DAOOLY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45614_length_2730_cov_4.820935"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..612)
                     /locus_tag="PLD15_11345"
     CDS             complement(151..612)
                     /locus_tag="PLD15_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64551.1"
                     /translation="MKRLVLFFLIIVAGTLAFSTARLIITLKDGTVIEYNMEDIESIL
                     YKVEEPKPLNSINMSLEKLVFAPGEEIKLRFFIATTLSSNAWIGIVPSEIPHGSEAEN
                     DKHDLTFTYLNGRSEGTLTFKAPAKPGAYDFRLHDTDNNGKELDFLTFEVK"
     gene            910..1281
                     /locus_tag="PLD15_11350"
     CDS             910..1281
                     /locus_tag="PLD15_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPB64552.1"
                     /translation="MKIAIPITDNAGLESRISEHFGHAPYFAFVVAENGGILSHEIVA
                     NPFEEHQPGQIPDFVKSHDAHVIITRGMGGRAKQFFQAMGIEAITGAGGTVKEIVESY
                     LDGKLQNRDYEPEGHGQHHNH"
     gene            1312..1674
                     /locus_tag="PLD15_11355"
     CDS             1312..1674
                     /locus_tag="PLD15_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPB64553.1"
                     /translation="MRSMRGSRMGMGRPWIELYLLLLIAEKPAHGYELSARLEEFEIP
                     IFSVGQMGNLYRVLSNLEEMGLIASEWDTEEAGPARKTYRITTPGLEYLKNAEIRIGR
                     FRDNINGFLKRIEQLKKV"
     gene            complement(1683..2450)
                     /locus_tag="PLD15_11360"
     CDS             complement(1683..2450)
                     /locus_tag="PLD15_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPB64554.1"
                     /translation="MTKLERHEKILKLIASNSSISVNELCKLVNTSHATIRRDLAELE
                     SEGLLERFHGGAVLSSKKRFEVSFSRRINQHNVEKLKIATEALERIEDGETIILDAGT
                     TTLQIAKLLVKSGKHVQIITNSIAIAQETADYIDPGVTLLGGNLDWHNMSTVGLVAIE
                     YLRTVMVDKAFIGANSVDIERGVFAVDESSAFLNRAMVASSRQVFVVCDSSKFQRIAP
                     FKSVDLDEVDCLITDGLIDRETAAKLSGRLNLVIAKD"
BASE COUNT          633 a          662 c          701 g          734 t
ORIGIN      
        1 tggacttgaa ccagcgacct tctgcgcccc atgcagacac gcttccaact gcgccacgcc
       61 ccgtactgga aaattgtatc ataaccggca ctctctttca agagcgaggc ggcaccgggc
      121 gcggtctttt cgacgctccc ccgattcact ctatttgact tcgaaggtca gaaaatccag
      181 ttccttcccg ttgttgtcgg tatcgtggag cctgaaatcg tacgccccgg gttttgcggg
      241 agccttgaag gtcagcgtcc cttcgctcct gccgttcagg tatgtaaaag tgaggtcgtg
      301 tttgtcgttt tcggcctcgc tgccgtgggg tatttcggaa ggcactattc cgatccacgc
      361 atttgaagag agcgtcgtcg cgatgaagaa acgaagcttg atctcctcgc cgggagcgaa
      421 gaccagcttt tcgagagaca tgttgatcga gttgagtggt ttcggctctt cgactttgta
      481 cagtatgctc tcgatatcct ccatgttgta ttcgatcacg gtgccgtctt tcagggttat
      541 tattagcctg gcggtagaga aggccagtgt gcctgcaact attatcagga aaaataaaac
      601 taatctcttc acagaaccac ctccagttga gattataaaa cattggcggt cgttatcaca
      661 gctttcacaa atgtgatgat aaaatttctc gaggaggtgt ttgcgatgac acttactagt
      721 gcacttgtcg cggtatcggt tctgcttacg atcgtggggc ttgtatctac aatctatatg
      781 atgattctga tctccaaact ctgattctct atcttgatac cggctcgctt gtttctccgg
      841 aggggttgta cgacggtcta agtgtaatga tataatatat gtaatataaa tatatttagg
      901 aggagagaca tgaagatagc tatacccata acagataacg ccggtcttga atcgagaata
      961 tccgagcatt tcggacacgc cccttacttc gcgttcgtcg tcgcggaaaa cggtggtatt
     1021 ctctcccatg agatagtcgc caaccccttc gaagaacacc agcccggtca gatccctgat
     1081 ttcgtcaaga gccacgacgc ccacgttata atcacgcgcg gaatgggcgg ccgggcgaag
     1141 cagttcttcc aggctatggg aattgaggcc ataaccggcg ccggagggac agtgaaggag
     1201 atagtcgaaa gttatctcga tggcaagctg cagaacagag attacgagcc cgaaggtcac
     1261 ggacagcacc acaatcactg aggctcttaa aaggcgcaca ggaggagaga gatgagaagt
     1321 atgaggggaa gtagaatggg gatgggaagg ccctggatag agctttacct gctgttgctc
     1381 atagccgaga agccggccca cgggtacgag ctttcagcca gactggaaga gtttgagatc
     1441 cctatattca gcgtcggtca gatgggaaac ctctaccgcg tgctgtcgaa cctcgaagag
     1501 atggggttga tagcttctga atgggacacc gaggaggccg gaccggccag aaaaacttac
     1561 aggataacca cgccgggcct cgaatacctc aagaacgccg agataaggat aggaaggttc
     1621 agggacaaca tcaacggctt tttgaagaga atagaacagc ttaagaaggt ttgagctggc
     1681 ggtcaatctt tcgctatcac caggttcaac cttccggaga gtttggcggc cgtctccctg
     1741 tctatgaggc cgtcggttat caggcaatcg acctcgtcca gatccaccga tttgaaggga
     1801 gcgattctct ggaatttgct ggaatcacag accacgaaga cctgtctgga cgaagctacc
     1861 atggccctgt ttagaaaggc gctcgattcg tccacggcga agaccccgcg ctcgatatct
     1921 accgagttgg cccctatgaa ggccttatcc accatgacgg ttctaagata ctctatcgcg
     1981 acgagaccga cggtcgacat attgtgccag tcgaggttcc cgccaagcag cgtaacgcct
     2041 ggatctatgt aatcggcggt ttcctgtgcg atcgctatgc tgttggttat tatctgaacg
     2101 tgctttccgc ttttgaccag cagtttcgcg atctggagag tcgtggtgcc ggcgtccagt
     2161 attatcgtct ctccgtcttc gattctttcg agcgcctccg tggcgatttt gagcttttcc
     2221 acgttgtgct ggttgatacg cctgctgaaa gagacctcga accttttttt gctgctcaag
     2281 accgcaccac cgtggaagcg ttcgagaagc ccttcgctct cgagctcggc cagatccctg
     2341 cgtatcgtcg cgtgggaagt gttcacgagt ttgcagagct cgttcacgct tatgctcgag
     2401 ttgctggcta tcagtttcaa tattttttcg tgtctttcca gtttggtcat tgactgatct
     2461 cccccaacgg tacgatattt tatgataaca caacgctcta cacattcccg gtttcggatc
     2521 gtattttcgc gggtatcgat ctttccgttg tttttcattc aattagagac gtttatccgt
     2581 ctttcttgcc ttttttcttg tttttactgt gtgtttctgt gtttggttta ttttgatcga
     2641 aatattagta taagatcata aacgatcatt aaaagcaaat cctttgtcca ttggagtggt
     2701 gtttataaaa agtatgaatt cagggataat
//
LOCUS       DAOOLY010000301         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_45621_length_2730_cov_3.669907, whole genome shotgun sequence.
ACCESSION   DAOOLY010000301 DAOOLY010000000
VERSION     DAOOLY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45621_length_2730_cov_3.669907"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1463
                     /locus_tag="PLD15_11365"
     CDS             96..1463
                     /locus_tag="PLD15_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB64555.1"
                     /translation="MFVGRKRELAFLEERFVKGRPELIILYGRRRVGKTELTKEFLRR
                     KRGIYYLGEKLPEQLQLKKLSEKVVEFFNEDIITEFSDWEMLFKYLAARHERYVLVID
                     EFPYLIEREPGIITAFQKGWDEYLLKSNVFLILTGSSVSVMENSVLSHKSPLYGRRTG
                     QLLLNQFTLSEIGDFFGKKYEFDDLLKIWGTLGGVPFYLEQFDSSLDYYGNVEEKILN
                     QGEVLFAEAEFLLNEELREPRNYFAILRAISLGKRKLGEIINETGIDKSSIMKYISVL
                     NLLGIVEKEVPVGEDPLKSKKGLYRIADNFFRFYFRYVFYYKDLVLTDQKERLLSILK
                     DTENQFFSLIYEDFAHSAIREITGSSYIEIGRWWDRNSEIDLVGMDREEKRILFGEVK
                     WSGNPVGTDVLATLREKANLPQWDNFKKKEYAIFSRSGFTKAVIDEAKSDRSLILVSG
                     NKRVV"
     gene            complement(1975..2382)
                     /locus_tag="PLD15_11370"
     CDS             complement(1975..2382)
                     /locus_tag="PLD15_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HPB64556.1"
                     /translation="MQEILKFVEKSAYIEEFKILDFKRGESFAYIKLEILFKNSSRIH
                     IREFITGERRKYSFHWVVNNLTTVRWDNSPHHKDIESFPHHLHINDELLPSDCTDSVA
                     ILTWIESLIEKKKDLSLETLLTEIKGLHLAKER"
     gene            complement(2357..>2730)
                     /locus_tag="PLD15_11375"
     CDS             complement(2357..>2730)
                     /locus_tag="PLD15_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64557.1"
                     /translation="VCGWLSKGNIICSLYYCSECYNSVEQLHRKRKEASNMIIISKEE
                     IAAFKKLDIMYQMNLLKEQIEKLERKHGCSLDQFRSLVNDSDEEFGLWDDLIEWEACQ
                     ASLNEMSSILENINAGNIEVR"
BASE COUNT          779 a          590 c          621 g          740 t
ORIGIN      
        1 agaacatcca tcctcggtgg actggcatta cacccttaat gatataatat actatatagt
       61 ataatactat atagtatttt aagtaggtga ggtcgatgtt tgtcggcaga aaaagggagc
      121 tagcctttct agaggagaga ttcgtaaagg gaagaccgga actaataatt ctctacggcc
      181 gcagaagagt aggaaagacg gaactcacca aagaattctt gagaaggaag cgaggcatct
      241 actacctggg ggagaagttg ccggagcagc ttcagctgaa gaagctctcc gagaaggtcg
      301 ttgaattctt caacgaagac ataataaccg aattctctga ctgggagatg ctgttcaagt
      361 acctggccgc aaggcatgag cgatacgttc tcgtaatcga tgaattccct taccttatag
      421 aaagagaacc tggcattatc acggctttcc agaagggttg ggacgaatac ttgttgaagt
      481 cgaacgtgtt tctcattcta actggttcaa gcgtctccgt catggaaaac agtgtcctga
      541 gccataagag cccgttatat ggcaggcgga ctggacagct ccttctgaat cagttcacgc
      601 tcagtgaaat cggggatttc ttcgggaaga aatacgaatt tgatgatctg cttaaaatat
      661 ggggtaccct tggaggagtt ccattctacc tggagcagtt cgacagcagt cttgattact
      721 atggaaatgt cgaggagaag atactaaacc agggcgaggt attgtttgcc gaagccgaat
      781 tccttctcaa cgaggagctc agagagccaa ggaactactt cgccatcttg agggcaatct
      841 cacttggaaa gaggaaactg ggcgagataa tcaacgaaac gggcatcgac aagagcagca
      901 tcatgaagta tatctctgtt ctcaatctgc tgggcatagt cgagaaagaa gtacctgtgg
      961 gcgaagaccc tcttaagagc aagaaagggc tctatagaat agccgacaac ttcttcagat
     1021 tctacttccg ctacgtcttc tactacaagg atctggtgct taccgatcag aaggaaaggc
     1081 tcctgagcat cttgaaagat accgaaaacc agttcttctc gctgatatat gaggatttcg
     1141 cgcacagcgc tatcagggag attaccggat cgagttacat cgaaatcggt cgctggtggg
     1201 ataggaacag tgaaatcgat ctggtgggta tggacagaga agagaaacga atattatttg
     1261 gagaggtcaa gtggagtgga aaccctgtcg gcaccgacgt tctcgcaact ttgcgtgaaa
     1321 aggcgaatct acctcaatgg gataacttca agaagaaaga gtacgcaatt ttcagcagat
     1381 ctggcttcac aaaagctgta atcgacgagg cgaaatccga tcgatctctg atactggttt
     1441 ctggaaacaa acgagtcgtt taatgtccct tcttcatatc tcgtcttcca gaatcccgag
     1501 aggccttcta taaagcttcc tgcaactgta aataaaccgc acgaccatac tggataggca
     1561 ccgcctgtag tgagagtaat atttgacgat cgacattggc caaaggacac ccaaactgca
     1621 cgattgtaaa attcctcttc gacaaatcat acaatatacg ctataagtat aattctattg
     1681 ggcataaata gggtagtaat aaccgagaaa gagcgccgag agttcgcttc gccctctttc
     1741 tctctccctc tccttttctc tctttctctc tcctcctcta gcaacgaagg actggtccat
     1801 gatctggagc ttctatatgc gactaagagc cagagatatc gataagtgag gacaggcaga
     1861 taggaaaaac ttgcgggtaa tgatgagctt tccgtaatgc tgaactgtga tctcgcatac
     1921 cgctctttga agttgaccca taagagctat actggaaaac tctgatagtt aatatcaacg
     1981 ctctttcgcc aaatggaggc cttttatctc tgtgagtaga gtttccaagg atagatcttt
     2041 tttcttttct atcaggcttt ctatccaagt taatatcgct acggaatcgg tgcagtctga
     2101 aggcagaagt tcatcgttaa tatgcaaatg gtgtggaaaa gattcaatat ccttgtgatg
     2161 gggggaatta tcccagcgca cggtggttag attattaacg acccaatgaa aggaatactt
     2221 tctccgttcg ccagttataa attccctgat atgtattctg gatgagttct tgaacaggat
     2281 ctcgagtttg atatatgcaa agctttcacc cctcttgaag tcaagaatct tgaactcttc
     2341 tatatatgca gatttttcaa cgaacttcaa tatttcctgc atttatattc tccagtatag
     2401 agctcatctc gttaaggctt gcctggcagg cttcccattc tataaggtcg tcccagagtc
     2461 caaattcctc atccgagtcg ttcacaagac ttctgaattg atccagagaa caaccgtgtt
     2521 ttcgctccag cttttcgatc tgctctttca ggaggttcat ctgatacata atatcgagct
     2581 tcttgaatgc agctatctct tctttggaga ttattatcat gttcgacgcc tccttccgtt
     2641 ttcgatgcaa ctgttccact gaattatagc attcactaca gtaatagagg gaacagatta
     2701 tgtttccctt agagagccac ccacagacgt
//
LOCUS       DAOOLY010000302         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_45925_length_2716_cov_4.795941, whole genome shotgun sequence.
ACCESSION   DAOOLY010000302 DAOOLY010000000
VERSION     DAOOLY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45925_length_2716_cov_4.795941"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLD15_11380"
     CDS             complement(<1..338)
                     /locus_tag="PLD15_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64558.1"
                     /translation="MKRKRVSLYIGISIIASLLAMMVVSLFYTPYPVTEMNRDERFAP
                     PSANHPLGTDSFGRDVLSRIMKGSQTAFFVGLIAVSVGGLFGVLVGAIAGYFGGMLDD
                     VRIYDYAVSSV"
     gene            complement(349..1290)
                     /locus_tag="PLD15_11385"
     CDS             complement(349..1290)
                     /locus_tag="PLD15_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64559.1"
                     /translation="MYIYLRKLFAMLATVFLVSMITFITFEIIPGDPVLARLGVDADE
                     AQIRALSEELGLDEPLSKRFFGWITGLFTGDLGQSIRYSRPVSELIFDRLPVTLSLAL
                     ISMVLIVIIGVPLGILSARYGDRLPGISISIVSQIGMAVPSFWTGIILMYIFGLTLRW
                     VSPGGYTPWSVDPVEAFKSLLLPAVAIALPSIATVIRYTRNTVMEQMKNDYVRLAFSK
                     GLKMNAVLFRHVLKNALIPVITVLGMITANILGGSIVIEQVFTLPGVGRLLINAISVR
                     DLPLVQGMVLYISLVIVVTNFLIDIVYTLIDPRIKLR"
     gene            complement(1305..>2716)
                     /locus_tag="PLD15_11390"
     CDS             complement(1305..>2716)
                     /locus_tag="PLD15_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64560.1"
                     /translation="ERIVVAVMQDPDFLDPHRAAASGTYEMTFNVFEGLLKPDSKGNV
                     IPAIAESYSISPDGLVYTFKLREGVKFHNGKEVTMNDILYSLNRLKGSGEVRGLSSDF
                     EKFVSEIEATDDITVEITLNTLNTDFLEKFIIAIIPADNPDHERNPIGTGPFKFVTYQ
                     PGQRVVIAKFDEYWNPDLPLVDEVEFRIIPDNQAALMSFMAGEVQMLPRLDAIQAEIL
                     RGRYNLISAEQNMVQLMAMNLAREPFDDLKVRQAINLAIDKEEIIEIVANGYGTVLGS
                     NMSPIMERYYQEGLEDYYKTDIVAARSLLAQAGYPEGFKTTITVPSNYQFHVDTAQVI
                     VEQLKKVGITAEIELIEWSIWLDRVYTGRDYEMTIVGLTGKLSAYDILKRYLSDYPRD
                     FYNYFNPRYDELVNSAIKQTDIDKKAGLFKQAQVLLTEEAVAVYIMDPNFIVALASNL
                     FGYNVYPLYVQDMSTLYYK"
BASE COUNT          605 a          761 c          696 g          654 t
ORIGIN      
        1 acactgctga ccgcgtaatc ataaatccgc acatcgtcca gcatcccgcc gaaataaccg
       61 gcgatggccc ctacgagcac tccaaagaga cctcccaccg ataccgctat caggcccacg
      121 aagaaggccg tctgcgaacc tttcattatc ctgctgagaa cgtctctacc gaagctgtcc
      181 gttcccagcg ggtggttggc ggagggaggg gcaaacctct cgtcgcggtt catctccgtt
      241 accggatacg gcgtgtagaa aagactcacc accatcatgg cgagcaacga agcgattatg
      301 gatattccta tgtaaagact cactctcttc ctcttcatcg ctcctccgct atctgagctt
      361 gattctggga tcgatcagcg tatagacgat atctatcaga aaattcgtta cgactatcac
      421 cagcgaaatg tacagcacca tcccctggac cagcgggagg tcgcgcaccg atatcgcgtt
      481 gatcaggagt ctcccgactc cgggcagagt gaagacctgt tctatcacta tgctgccgcc
      541 gagaatgttg gccgtgatca ttccgagaac ggtaataacc ggaatcagcg cgttcttcaa
      601 aacgtgtctg aaaagcacgg cgttcatctt cagacctttg gagaaggcga gtctgacgta
      661 atcgttcttc atctgctcca tcacggtgtt cctggtgtat cttatcacgg tcgctatcga
      721 agggagggcg atcgccactg ccggaagcag aagcgatttg aaagcctcca cagggtcgac
      781 gctccagggt gtgtaacccc ccggtgagac ccacctgaga gtgagaccga aaatgtacat
      841 caggattatg ccggtccaga aggaaggcac ggccatccct atctgcgaga cgatggagat
      901 tgaaataccg ggcagtctgt cgccgtacct ggcgctcaag attcccagcg gcacaccgat
      961 aatcacgatc aaaaccatgg agatcaatgc cagcgagagc gtcaccggca atctgtcgaa
     1021 gatcagttcg gagacgggtc tcgagtatct tatcgattga cccagatcgc ccgtgaagag
     1081 accggtgatc cacccgaaga accttttgga aagcggctcg tccagtccca gctcctctga
     1141 aagcgctctg atctgggcct cgtcggcatc tactcccagc ctggccagga ccgggtctcc
     1201 cgggatgatc tcgaaggtga tgaacgttat catcgagacc aggaaaaccg tcgcgagcat
     1261 cgcaaatagc tttctgaggt agatgtacac gggtctctcc ggttctattt gtagtaaagt
     1321 gtcgacatat cctgcacgta cagcggatag acgttgtacc cgaaaaggtt cgacgccagt
     1381 gctactatga agttcgggtc cattatgtac acggcgacgg cctcctccgt gagcagcacc
     1441 tgcgcctgct tgaagagccc ggcctttttg tctatatcgg tctgctttat ggctgaattg
     1501 accagttcat cgtaccgggg gttgaagtag ttgtagaagt ccctcggata gtcgctcagg
     1561 tacctcttca agatgtcgta cgcgctgagc ttcccggtga gacccactat cgtcatctcg
     1621 tagtctctcc ccgtgtaaac cctgtccagc catatgctcc actctatgag ctcgatttcg
     1681 gccgtgatcc caaccttttt caattgctcg acgatcacct gggccgtatc gacgtggaac
     1741 tggtagttgg agggaacggt tatcgtcgtc ttgaaacctt ccgggtagcc ggcctgggcg
     1801 agaagcgatc tggccgcgac gatatcggtt ttgtagtaat cttcgagccc ttcctggtag
     1861 tatctctcca taataggcga catgttggaa cccagcaccg ttccgtaacc gttggcgact
     1921 atctctatga tctcttcctt gtctatggcc aggtttatag cctgcctcac cttaaggtca
     1981 tcgaaaggtt ccctcgcgag gttcatggcc ataagctgca ccatgttctg ttcggcgctt
     2041 atgaggttgt acctgcccct cagaatctcg gcctgtatcg cgtccagcct gggaagcatc
     2101 tgaacctccc cggccatgaa gctcatgagc gcggcctggt tgtccggtat tatcctgaac
     2161 tcgacctcgt ccaccagcgg aagatcggga ttccagtact cgtcgaactt tgcgattacc
     2221 acccgctggc ccggctggta agtgacgaat ttgaacgggc ctgtccctat gggattcctt
     2281 tcgtgatcag ggttgtctgc cggaattatc gcgattatga acttttcgag gaagtctgtg
     2341 ttcagagtgt tcagagtgat ctccacggtt atatcgtcgg tggcttcgat ttcggatacg
     2401 aacttctcga aatccgaaga aagtcccctg acttcgccgc tccccttcaa cctattcaaa
     2461 gagtatagaa tatcgttcat ggtaacctct ttgccattgt gaaatttgac cccctctctg
     2521 agtttgaaag tgtacacgag gccatcggga gagatggagt aactctcggc aatggccgga
     2581 atcacgttcc ccttcgaatc gggtttcagg agaccttcaa aaacgttgaa cgtcatctcg
     2641 tacgttcccg aagccgctgc cctgtgcgga tcgagaaagt ctgggtcctg catgacggca
     2701 acgactatcc tctcgg
//
LOCUS       DAOOLY010000303         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_45974_length_2714_cov_3.932305, whole genome shotgun sequence.
ACCESSION   DAOOLY010000303 DAOOLY010000000
VERSION     DAOOLY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45974_length_2714_cov_3.932305"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLD15_11395"
     CDS             <1..625
                     /locus_tag="PLD15_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPB64561.1"
                     /translation="VGIPFLVLIFLLAAPRIWPFGFPVLGLPLAFAICALVAWAMNYK
                     RVNILKVSKDTVSQLTPLLATTAVVGMLVQIMSFNGVKGLISMWIVTAPLIVVWIILP
                     FIIPISEGLLTYGGAMVIGVPLIWMLNSNGINPVIVLAGLSLLWPLGDGLPPTALIGR
                     LTVSTVGYKGSYGSFLKECIVPWIAITVVGMIMVIFANNLSFLMMAG"
     gene            638..790
                     /locus_tag="PLD15_11400"
     CDS             638..790
                     /locus_tag="PLD15_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64562.1"
                     /translation="MTFIMILYYVMTAYVVGMLIWNFIKTKDIKSEILYALALLPLIL
                     RLLRMK"
     gene            804..1949
                     /locus_tag="PLD15_11405"
     CDS             804..1949
                     /locus_tag="PLD15_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HPB64563.1"
                     /translation="MRKWIILAVLLLVVLAGGIPLYIHRNFKEEVVAGEGVTGFFKLS
                     KYFSGIEGTIADTDVFELKGSEEGGKMLIIAGTHESEPSAALAAFYLIENLKVDRGTV
                     YIIPHFNRSGSLGTQPGGGFPLYFSMETPWGEKTFRMGDRGFQALDQWPDPDVYIHYP
                     EGQAMSYIDARNTNRAWPGRPDGLYAEQVTYAAMEMIKKEKIDIVIDLHEAEAMYPVT
                     NCIVVPEKSMSYATGASFYVKGREKFENHVERSPTAFRGLSHREIGDWSDAYPFLLES
                     PGVHLDQITAAKTPELIMDGIDPFVLKAAEKGLLFVPYDENGFSMDRRVGQHSSVIQE
                     IVVQWSKKNPERGLTISAPRYKEIVDNGLGYFFKDPSKADKSDVYLQ"
     gene            2240..2548
                     /locus_tag="PLD15_11410"
     CDS             2240..2548
                     /locus_tag="PLD15_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HPB64564.1"
                     /translation="MERIIVTVEPDLEGLIDGFLVSRQLDSEKLQTAIVEHDFEEIRN
                     IGSSLKEAGSSCGFDFISIIGTKIEMAGTQKNKLGAEIALDAFNWYLGRIRTEVFGEG
                     "
BASE COUNT          711 a          655 c          696 g          652 t
ORIGIN      
        1 cgttggaatt cccttcctgg ttcttatatt cctgctcgcc gccccgagaa tctggccctt
       61 cggattcccc gttctcggtc tgcctctggc gtttgcgata tgcgcgcttg ttgcctgggc
      121 aatgaactac aagcgagtca atatactcaa ggtaagcaaa gacacggttt ctcagttgac
      181 acctctactg gcaaccaccg cggtcgttgg aatgcttgtc cagataatga gtttcaacgg
      241 agtcaaaggc ttgatatcca tgtggatcgt cacggctcct ctgatcgtcg tctggataat
      301 cctgccattc ataataccga tctccgaagg attgctcacc tacggaggcg cgatggttat
      361 cggtgttcct ctcatctgga tgctgaactc taacggtata aatcccgtta tagttctggc
      421 cgggctgagt ctgctgtggc ctctcggtga cggattgcca ccgaccgcac tcatcggacg
      481 cctgacagta agtaccgtcg gctacaaggg ctcctacggt tccttcttga aggagtgtat
      541 agttccctgg atagctatta ccgtcgtggg aatgataatg gttatcttcg ccaacaacct
      601 cagcttcctg atgatggccg gttaaggagg aattgtcatg acttttatta tgatccttta
      661 ttatgtgatg acagcctatg tagtgggcat gcttatatgg aatttcatca aaaccaagga
      721 cataaaaagc gaaatactct acgcattggc ccttctaccg cttattctca gattactgag
      781 aatgaaatag gagggatttt atgatgagaa aatggattat tctggcagta ctgctgctgg
      841 tggttcttgc cggcggaatt cctctgtaca tacatcgaaa cttcaaggaa gaggtcgtcg
      901 caggtgaggg tgtaaccggc tttttcaagc tgagcaaata cttcagcggc atcgagggaa
      961 cgatagccga taccgacgtc ttcgaattga aaggttccga agagggaggg aagatgctca
     1021 taatagccgg tacgcacgag agcgagccct ctgcggcgct ggcggcattc tatcttattg
     1081 aaaatttgaa ggtcgatcgg ggaacagtgt acataatccc tcacttcaac agaagcggtt
     1141 cgctcggcac tcagcccgga ggcggtttcc cgctgtattt cagcatggaa actccctggg
     1201 gagagaagac cttcagaatg ggtgacagag gatttcaggc tctcgatcag tggcccgacc
     1261 ccgatgtcta tatccactat cccgaaggtc aggccatgtc ctacatagac gccagaaaca
     1321 ccaacagagc ctggccggga agaccggacg gactctatgc agaacaggta acctacgcag
     1381 ccatggagat gataaagaag gaaaagatcg acatcgtcat cgatctccac gaagccgagg
     1441 ccatgtatcc ggtcaccaac tgtatagtcg taccggagaa atcgatgagt tacgccaccg
     1501 gcgcctcgtt ctacgtcaag ggcagggaaa agttcgaaaa ccacgtcgaa cgctccccga
     1561 cggctttcag gggactctct cacagagaaa tcggcgactg gtcggacgct taccccttcc
     1621 tgctggaatc tcccggtgtc catctggacc agataaccgc ggccaagact ccggaactga
     1681 taatggacgg tatcgatccc ttcgttctca aagccgccga aaagggactg ctgtttgttc
     1741 cttacgacga aaacggcttc tcgatggata gaagggtagg tcaacactct tcggttattc
     1801 aggaaatagt cgtgcagtgg tcgaagaaga accccgagag aggtctaacc atttcggctc
     1861 ccagatacaa ggaaatcgtt gacaacggtc tcggatattt cttcaaagat ccttccaaag
     1921 ctgacaagag cgacgtgtat ttacagtaag ttatctctat ctctcgagtc cggggaaaaa
     1981 acattccccg gactttttta ttccccgttc cacagtgttt tcgaagagtc ttcgttacca
     2041 ctttgaaata tccctttgac gaatcttata ttgtatcatt ggtatatcct gacagaatcc
     2101 agaccgggag aattgcagta tggacggcag gtctggagac aactggaaat agaccgactc
     2161 acacacggcc atggcgatag tacagagcat tcagggtgaa aatctggcag gaaagttgtg
     2221 agaacatgga ggatagccaa tggaaaggat catcgttacg gtggagcccg acctggaagg
     2281 tctcatagat ggatttctag ttagcaggca actcgattcc gagaaactcc agacggctat
     2341 tgtcgagcat gatttcgaag agataaggaa tatcggaagc tctctcaagg aagccggttc
     2401 cagttgcggg ttcgatttta tttcaatcat cggtaccaag atcgaaatgg ccggtaccca
     2461 gaaaaataaa ctgggcgccg aaatagccct ggacgctttc aactggtatc ttggaagaat
     2521 aaggacagaa gtcttcggag agggataata tgaagtgcgg aactatctcc gtggacgaca
     2581 tgagcaaagc gaaacctctt gaaatgaaaa ttccaccggg caatcccatt gtcgccataa
     2641 ctatgggcga ccccgcgggg ataggcccgg aaataactgc aaaagccgtt gccggtggcg
     2701 aaatttacga attt
//
LOCUS       DAOOLY010000304         2705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_46193_length_2705_cov_3.381509, whole genome shotgun sequence.
ACCESSION   DAOOLY010000304 DAOOLY010000000
VERSION     DAOOLY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46193_length_2705_cov_3.381509"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLD15_11415"
     CDS             complement(<1..209)
                     /locus_tag="PLD15_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64565.1"
                     /translation="MIKARGTFVRASIIFFLILTTVTLTITGCFLKPVTYTLTMFEPT
                     GQGEVEPTEGDHQYNEASVVNLTATP"
     gene            complement(492..1724)
                     /locus_tag="PLD15_11420"
     CDS             complement(492..1724)
                     /locus_tag="PLD15_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB64566.1"
                     /translation="MKEIYLILLRLLSKVAQIESHALQVAYIAKKIGEDIGFSSKRLN
                     TLGFLHDIGLLNPNKVNWMQRTHGIENATMTDWIALDNDLHENHSVVGARILSHIKGL
                     SYFADVIEKHHYQAMILDPENDHDVMAGIIHLADTVSVGLLMNTPGAEYVTRIKNIIA
                     SSDEFLEPVRESFNRLSSRKGFWWSSTDRDTLFDEFSSDLEENPLNNVDLQAVGDVLM
                     YMVDVKSSFTMNHTERISYLSRLLAERIHLGQERALEVFFAAKIHDLGKMATPISILE
                     KPGKLSGEELYIMQKHVFDSFLIVGGREAIEKHRLLEWGVDHHERLDGSGYPWGKKGY
                     ELGIESRIIMIADVFVALTEDRPYRKGLSIKESLNVIGRQVEQGMLDDYVFEKLKDLI
                     DEGLDFSKVERTRNPFLP"
BASE COUNT          701 a          589 c          566 g          849 t
ORIGIN      
        1 ggggtagccg taaggttaac caccgatgcc tcattgtact gatggtcgcc ctcggtcggc
       61 tcgacctctc cctgtccggt cggctcgaac atcgtaagag tataggttac cggtttgaga
      121 aagcatccgg ttattgttag tgttaccgtt gtcaatatca gaaagaaaat aattgaagca
      181 cgtacaaacg tacctctagc ttttatcatc attgagtcct ccctttgact tgccagatag
      241 ataccgcctt ttgtgggttc aaaatcgata ctgttctctg ttattatata tcgtcgatct
      301 tatggaattt atcgccacgg atagtctggg aaacaccttt ggaaaggctg atagtatttg
      361 tatctgttca aagccgatct caccagagga gattatgtga gttccggtta aagtagaaaa
      421 cctggatatg gcttttcttt tcagacatta cctctatctt ttgaaagttc cgtggcacct
      481 ttcacagtct ttcagggaag gaagggattt ctcgtcctct cgactttcga gaaatcaagc
      541 ccttcgtcga tcagatcttt gagcttttca aaaacataat cgtcgagcat accctgttca
      601 acctgtctgc caataacgtt cagcgactct tttatagaca gaccttttct gtaaggtctg
      661 tcctcggtca acgctacgaa gacatctgca atcattatga tcctcgactc tataccgagt
      721 tcatatccct tcttacccca ggggtagcca ctaccgtcga gtctttcatg gtgatccacg
      781 ccccactcca gcagcctgtg tttttcgatg gcttctcttc cgcccacgat gaggaaggaa
      841 tcgaagacat gcttctgcat tatgtacagc tcttcacctg aaagcttccc gggtttttcg
      901 agtatagaga taggtgtcgc catctttcca aggtcgtgta tcttcgcggc aaagaaaacc
      961 tccagtgctc tctcctgacc gagatggatc ctttcggcaa gaagcctgga aaggtacgag
     1021 atcctttctg tatggttcat cgtgaaactg ctcttgacat cgaccatgta catgagaaca
     1081 tctccaaccg cttgaagatc gacgttgtta agcgggtttt cttcaaggtc ggagctgaat
     1141 tcatcgaaga gagtgtctct atcggtcgaa gaccaccaga agccttttct ggaagagagc
     1201 ctgttgaagc tctccctaac cggttcgaga aactcatccg aactggctat tatgtttttg
     1261 attctcgtga catattcggc gcccggcgta ttcataagaa gacctacgct gactgtatcg
     1321 gccaggtgaa taatacctgc catcacatcg tgatcgttct ccgggtcgag gatcatggcc
     1381 tggtagtgat gcttctcgat cacgtcggcg aagtaagaaa gaccttttat atggctcaaa
     1441 atcctggcac cgacgaccga atggttctcg tggagatcgt tatcgagggc gatccaatcg
     1501 gtcatggttg cgttctctat accgtgagtt ctctgcatcc agttaacctt gttgggattt
     1561 agaagcccga tatcgtgcag aaagcccagt gtattgagtc ttttggaact aaagcctata
     1621 tcctcgccga tcttcttggc tatgtatgct acctggagag cgtgagactc tatctgggct
     1681 accttcgaga gaagtctgag caagatcaaa tatatctctt tcatattgaa tttcacgctg
     1741 aatcacactc ccaccggcga taattagcag ttcagatgag attccatctg cttaagatta
     1801 tacagcagaa aaaacgtcgt cagtctgaat ctagggtacg aggaagggtt tcgtcacctg
     1861 ccagaacttc ttgagggctg cgcgtgcctt cggttatgac agccagtatt acaaacaagt
     1921 gcaatctcaa aaaagagctc atcactatct cttactcata ttgtgagttt cccaatggtt
     1981 cgttccggat tgtacagagc aatttatcta aagcacatac gttgggtttt gaagaataga
     2041 aactcgaatc tttcttatat tggcctatag ccttcattta aagagattct ttgcgggaat
     2101 actttctcta ttagtttgaa tttaattaac aatattgata atatgatctt tattatagta
     2161 aatatcgcaa taaatgagat cataatatct caataaaata actatcagaa acacagttcc
     2221 gatttttact tctaatccta gctgataagc tcttatttgc tcagaatggc tctcttggaa
     2281 gctgagtgtt tttgttgaat ttggagtcga aaatataaaa ttgttgctgt ggcctcttga
     2341 gagccgattt tctaaatggg ggattaaaaa cacctgggta gttatatact tcaccaaaat
     2401 ctataaattt atatgtatta aaaattttat ttggttgacc actaatatta tttgtcatat
     2461 ttgtttaacc ataatttttg tattcgccgt cattattatc ttatttgcct tcttacttct
     2521 tcgcttagtt ttagtccttg gacttcagga cattatgatt ttctcttatt gcgtttagtg
     2581 atttggagaa tagtacttgt tcagtttctg tcttgttctg tcttgagttg aatacgctaa
     2641 aagcggccca aactgcattt cagcagaata atccagtaaa aaacgagagg gaggtggttg
     2701 aacca
//
LOCUS       DAOOLY010000305         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_46277_length_2702_cov_4.356630, whole genome shotgun sequence.
ACCESSION   DAOOLY010000305 DAOOLY010000000
VERSION     DAOOLY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46277_length_2702_cov_4.356630"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLD15_11425"
     CDS             complement(<1..578)
                     /locus_tag="PLD15_11425"
                     /inference="COORDINATES: protein motif:HMM:NF023524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HPB64567.1"
                     /translation="MQEAINRVLDRYLIEKKKSFASNRLADYLRGHVSETVQKAVDSK
                     YPGQFKVSASAGQGRWAEIPWIGVFDKDITESPMRGYFVMYFFRSDMSRVFLSLNQGW
                     MFFRDTYGVELAKEKIATAARAYRKMLGPIGQFGYESISLGTERELGTGYELGHICGK
                     RYERGEVPDDETLRRDLLDIVDIYRRLKVIIGT"
     gene            complement(891..2399)
                     /locus_tag="PLD15_11430"
     CDS             complement(891..2399)
                     /locus_tag="PLD15_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPB64568.1"
                     /translation="MRRSKFLILAVILLSILSFSTTIKMYHVTDYHSHAIPFYSEGQH
                     GFGGIARIITYLREKSVDENTLIFCGGDMINLGTPAWSDKFHAIELPWFNNIFDAMAF
                     GNHDSEYGAEDFQEVWKQLTYPILGGNILDVNGSPVFECLGKRYLVFEVEGKRIGVFA
                     LAGSDYDSLVKPAARPLEGATFGDFMGTAAEIVEEMKAEEVDLIVFIGHAEYEETLEM
                     ARKVEGIDLIFGTHSHLKIPLTKIDGTDTYFISPYQYGTYAAEVVVYFGADGKKNISG
                     SLIPMDSSLPEDPIIAGKVEKLKSELESDPAFTHLFEWIGEVETELSASNVNTGESVL
                     GNFVMDVIREASGANVALSTSSSFRASIAPGATVYEDLKNALPYVNIIYVYEVKGDTL
                     EKLLNHSISQSGTGFFSQVSGVRFVISDGKATAVEVQADLECPDSFEPLNLEKTYLVA
                     TTNYQGLFAPGYKDLFAGLKYTDTGLDVQKLVKEYMQNNSPVHAALDGRIIK"
BASE COUNT          677 a          714 c          588 g          723 t
ORIGIN      
        1 gtaccgataa tcactttcag tcttctgtaa atatccacga tatccagcag gtctcttcta
       61 agcgtttcat cgtcgggaac ctctcccctt tcgtatctct taccgcagat gtggcccagc
      121 tcgtacccag ttcccagctc tctctccgtt ccgagcgata tactctcata gccgaactgg
      181 ccgatgggtc cgagcatctt cctgtaagcc ctcgccgcag tggctatctt ctccttggcc
      241 agctccactc cataagtatc tctgaaaaac atccagccct gattgagaga aaggaatact
      301 ctggacatat ccgacctgaa gaagtacatc acaaaataac ctcgcatcgg agattcggta
      361 atgtctttat cgaatactcc tatccatgga atctccgccc accttccctg accggcggaa
      421 gccgatactt tgaactgacc cgggtacttc gaatcgacgg ctttttgaac cgtttcgctg
      481 acatgacccc tcagataatc tgccagcctg ttgcttgcga agcttttttt cttttcaata
      541 agatacctat caagaaccct gttaatggct tcctgcataa gccctcctca ttgataatcg
      601 ctcatcttat gagcaacatt aaaaagccgc ttgactaact agagcgtatc attaaactaa
      661 ttcatattcc aaatgaaagc gcctgtcgat aacggaaccg gcttctcgtt cgaacaaggg
      721 cgacagatgt caagatcatg agttaagaga aatgatgtca aaggccggaa gaacaaaaga
      781 gccgtcagtc gttgttttat ccagaacaac tgactcctgc tcggaaagag cgttaagagg
      841 cgttaatagc tccggctcct ggctttaaat ccttccgacc cctgatttag ctatttaatt
      901 attctcccat cgagcgccgc atgaacggga ctgttgttct gcatgtactc tttgacaagt
      961 ttttggacat ccagtccagt atcggtgtat tttaggcctg caaacaggtc tttgtagccc
     1021 ggagcgaaga gtccctggta attggttgtg gcgaccaggt aggtcttctc aagattcaga
     1081 ggctcgaagc tgtccggaca ttccagatcc gcctgaactt caactgccgt agcctttccg
     1141 tcgcttatca cgaagcgtac tccggagacc tgtgagaaga atccggtgcc gctctggctt
     1201 atcgagtgat tgagaagctt ctctagcgtg tcccctttga cttcatagac gtagattata
     1261 ttgacgtaag gaagggcgtt cttcagatct tcgtatacgg tagctcctgg agctatagac
     1321 gccctgaagc tgctcgaagt cgagagagcc acgttcgccc ccgatgcctc ccttatgacg
     1381 tccatcacga aattacccag caccgattct cccgtattta cgtttgaagc ggacagttcg
     1441 gtctcgactt cacctatcca ctcgaacaga tgggtgaatg ccggatcgct ttccagctcc
     1501 gactttagct tttcgacttt cccggcaata attggatctt caggcagaga agaatccatt
     1561 gggataagtg aacccgagat attcttcttg ccatccgctc caaagtaaac aactacctcg
     1621 gctgcataag ttccgtactg gtaaggcgag ataaagtacg tatccgttcc atcgatcttt
     1681 gtgagaggga tcttgagatg gctgtgcgtg ccgaagatca ggtctattcc ttctactttc
     1741 ctcgccattt ccagtgtctc ctcgtattcg gcgtgtccta tgaatacaat caaatctact
     1801 tcttcggcct tcatttcttc gacgatctcg gcggctgtcc ccataaaatc tccaaatgtg
     1861 gctccctcaa gcggcctcgc ggcaggtttc acaagactgt cgtaatctga gccggccagc
     1921 gcgaagacac cgatcctctt tccttcaact tcaaaaacaa gataccgctt accgaggcac
     1981 tcgaagactg gcgacccgtt tacatccaga atgttgcctc caagaatcgg ataggtcaat
     2041 tgcttccaaa cttcctggaa gtcctctgcg ccatactccg aatcgtgatt tccgaatgcc
     2101 attgcgtcga agatattgtt gaaccacggc aattcgatcg cgtggaactt gtcggaccac
     2161 gccggagttc cgaggtttat catatctccg ccgcaaaata taagcgtgtt ttcgtccacg
     2221 gacttttctc taaggtaagt gatgatcctg gcgattccac caaatccatg ctgcccttca
     2281 gagtagaagg gaatcgcatg agaatggtag tcagtgacat gatacatctt gatagtagta
     2341 gagaacgaca ggatcgaaag gagtatgaca gcgagaatta agaatttaga tcttctcatt
     2401 ccaacacctc ctcataacag ttaatttaat tctagcatcg ccgagagtta caaaaccctt
     2461 cttataagac ctgttacaga gtgaaaatct gtcagactta caaagtatca ctctttgata
     2521 aagagagcgt ctcgtccgtc tcgttctctc tctaacataa accgatgtgt ctggggtctg
     2581 gggtgtagta agagcgagat cccgtataag atcactacgg gatgaccctg aataggatac
     2641 ctgaataaga gccctgaata ggagcatttc aggacaggct cctcagggca ggctctcccc
     2701 tt
//
LOCUS       DAOOLY010000306         2660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_47271_length_2660_cov_7.502111, whole genome shotgun sequence.
ACCESSION   DAOOLY010000306 DAOOLY010000000
VERSION     DAOOLY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47271_length_2660_cov_7.502111"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PLD15_11435"
     CDS             complement(<1..1417)
                     /locus_tag="PLD15_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB64569.1"
                     /translation="MHKVSLVLFLFMVVAVAATTGSIELYGNEIFFRINEAEGLPIVV
                     IGDGPGISNRYLEDILPARKTVFYDSPGIGKSSNAGSLDLSVEYYISELSAIVHELEL
                     ERFHLVGHGFGSLVAVYYALARPAGLSSLVLVNPILSIPQLDLLLEEHALQNDTEPDK
                     LKLLFLNYRGDIEAKLKTTVEDVCRDTYNQFWGDGPVVTGKLRGLDISSGLSDLRMPI
                     LICTGLYNFPGVDKIIDYQKLSPTSEFVTFINSAHFPMLEESEGFNMIVGDFLRRVEA
                     EHENSPAPDSSGKAVDTLSGVVSLGEKDNGVDMDLEVGRDFSVSLPSSPGTGYSWKLE
                     EFDGRVLRLLSDPFYEEPGTTGGGYQVFQFRVIGPGEADISLSYCSCWNDEPIRTYRV
                     GISVPLQEREALSIDESGNGKSFSVDLAVPLEVSLNVTTGTGMSWRITSTTSSILRQT
                     KENEYSIPENTPAVGGTVQQRY"
     gene            complement(1530..2540)
                     /locus_tag="PLD15_11440"
     CDS             complement(1530..2540)
                     /locus_tag="PLD15_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPB64570.1"
                     /translation="MDIIALLIEFVKAQGYTSSFCRNLLSCILTVMVFNLEPTISRIA
                     LFLPVNAELRHRRKHLQRFLLMLDERIFKVLFKLVLHVLGKPSRIVVAIDWTQIGKRY
                     LFLAAITIDGRTLPIAFLLRENKSVQDKKEMVSLIGRVISDPVEVTIVADGEFFSPKF
                     LDCVKRSGFHYVIRTRKNVLFNGTALVKYSDSTKDLWFGKYTSSGYCARVIINRRGKD
                     SYFLVTDLRYSEETILEIYLKRFWIEETFRDYKTYMGFGKYSKSQSIDRLFGLAVIYS
                     FSYVFAYHTSKSLVRDNDLSWYLCARTFQTYLACNSIFLSLLNSIPGLFRRPPSSRSF
                     FR"
BASE COUNT          689 a          665 c          593 g          713 t
ORIGIN      
        1 agtaacgctg ctgaacggtc ccgccgacgg ccggtgtgtt ttcgggtatc gaatactcgt
       61 tttccttcgt ctgacggagg atagaagagg tagtggaggt tatcctccat gacatgccag
      121 ttcctgttgt cacgttcagt gaaacctcca gaggtacggc cagatctacc gagaaactct
      181 tcccgttacc ggactcatcg atcgatagag cctccctttc ctgaagcggt acagagattc
      241 cgaccctgta ggtccttatc ggttcatcgt tccagcacga gcaataagac agagaaatat
      301 cggcctctcc ggggcctatc accctgaact gaaagacctg gtagccgccg ccggtcgttc
      361 ccggttcttc gtagaacggg tcggagagaa gtctcaagac ccgtccgtcg aactcctcta
      421 gcttccagga gtaaccggtt cccggactcg aagggagtga gacgctgaaa tcacgaccaa
      481 cttcaaggtc catatcgacc ccgttgtctt tctcaccgag cgagacaact cccgaaagag
      541 tatcaacggc ttttccagat gaatcaggcg cagggctgtt ctcgtgttca gcttcaactc
      601 tccggaggaa atctccgact atcatgttga acccctcact ctcttcaagc atgggaaagt
      661 gtgcgctgtt tatgaatgtg acgaactcgc tcgtcggaga gagtttctgg taatctatga
      721 ttttatcgac tcctggaaaa ttgtacaggc ctgtgcagat aagtatcggc attctgagat
      781 cggatagacc cgacgagata tccagccccc tcaacttacc ggttactacc ggtccgtcac
      841 cccagaactg attgtaagtg tcgcggcata cgtcctccac ggtcgtcttc agttttgcct
      901 ctatatcgcc tctgtaattc aagaaaagca gcttcaactt atcgggttcg gtatcgttct
      961 ggagagcatg ttcttcgagc agcagatcta gctgaggaat cgagagaatt ggattcacaa
     1021 gcaccaggct cgacagtcca gccggtctgg ccagagcgta ataaacggct accagacttc
     1081 caaagccgtg tcccaccaaa tggaatctct caagttccag ttcgtgaacg atcgccgaca
     1141 attcggatat gtaatactcc acgctcaaat ctaaggagcc ggcgttgctg gattttccta
     1201 ttccgggcga atcgtagaaa accgtttttc ttgctggaag tatgtcttcg agatatcgat
     1261 tgcttattcc cggtccgtca cctatcacaa cgatgggaag gccttcggct tcgttgattc
     1321 tgaagaatat ctcattaccg taaagctcta tactccccgt ggtggcggcc acagcgacca
     1381 ccataaaaag aaaaagaact aaagatactt tatgcaattg aacctaacct cccctgatga
     1441 atggacatcg gtacagtaaa attatataac gtgctgaggt tagcttcttt gccgggggct
     1501 gaccctgtcc cattttaaca aagaaaaggt taacggaaga aagaacggga agagggagga
     1561 cgtctgaaaa gaccgggaat agaattcaac aagctaagaa agatggaatt gcatgcaaga
     1621 tatgtctgaa aggttcttgc acaaaggtac caggagagat cgttgtctcg aacaaggctc
     1681 ttgctcgtgt gataagcgaa aacgtatgag aagctgtata taaccgcaag cccgaagagt
     1741 ctgtctatcg attgagactt cgagtacttg ccaaaaccca tataagtctt gtagtctctg
     1801 aaagtctctt caatccagaa tctctttaga tatatctcaa gaatcgtctc ttcagaatat
     1861 ctcagatcgg tcaccaggaa gtatgagtcc ttaccccttc tgttaatgat gactctcgca
     1921 caatagcctg agctggtgta cttgccaaac caaaggtctt ttgttgaatc actgtatttc
     1981 acaagcgctg ttccattgaa gagtacgttc ttcctggttc ttatcacata atgaaaaccg
     2041 cttctcttca cacaatcgag gaacttcgga ctgaagaact ccccatctgc aactatcgtc
     2101 acttcaactg gatcgcttat cactctgcct atgagagaaa ccatctcttt cttgtcctgt
     2161 actgacttgt tctctctcag aagaaaggct atgggtagag tccttccatc gatggttatc
     2221 gctgcgagaa agaggtatcg cttccctatc tgtgtccagt cgatggccac cactattctc
     2281 gaaggctttc ctagaacatg caagaccagc ttgaacaata ccttgaatat cctttcatcc
     2341 aacatgagca agaatctctg cagatgcttc cttctgtgtc tcaactctgc attcaccggt
     2401 aagaagagcg ctattctgct tattgtaggc tcgagattga agaccatcac tgttaatatg
     2461 cagcttaaga gattcctaca aaaagagctt gtgtatccct gggcttttac aaattcgatc
     2521 aagagtgcta ttatatccat taggatgcct cctagctttt gatgggtatt acagcgtatt
     2581 tatcttaagc caggagcatc cttttttcaa tccctttttc ttctcttcat ctctttcctg
     2641 ttaacttggg gcagggtcag
//
LOCUS       DAOOLY010000307         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_47721_length_2642_cov_4.241979, whole genome shotgun sequence.
ACCESSION   DAOOLY010000307 DAOOLY010000000
VERSION     DAOOLY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47721_length_2642_cov_4.241979"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLD15_11445"
     CDS             complement(<1..276)
                     /locus_tag="PLD15_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB64571.1"
                     /translation="MKELTVGALGGLGGKMLVDFFSNACGVCAAVKRRRESLEADFPQ
                     WEFVSVDTKKHPEAAGEYSVFTVPTVLILLEGRELNRWSRYFSIEEIG"
     gene            338..880
                     /locus_tag="PLD15_11450"
     CDS             338..880
                     /locus_tag="PLD15_11450"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPB64572.1"
                     /translation="MESTTAVLVPVLRICGDDHLILTRRSRKISHPGQMSFPGGHREN
                     GETLLECAVREMEEEIGASPSRIEEVCPLGVTTTVTSGKVIVPFIGFIDRPFFRLNRS
                     EVEDLVFLSIPALKYVSPEPIVMPSGKVTIRYRFPGFIVWGATARIIESSLNRIVELI
                     DRRNRGMPLCNDGLIDNKKE"
     gene            885..2048
                     /locus_tag="PLD15_11455"
     CDS             885..2048
                     /locus_tag="PLD15_11455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPB64573.1"
                     /translation="MLDFVFSNATKIIFGRGTELKTGQEISRYGKKILLHYGGGSIKK
                     SGLYDRVIESLRKAGVEAIELGGAMPNPRLSLVREGIELCRKHGIDAILAVGGGSVID
                     SAKAIGIGVPYAGDVWDFYSTGRKVEEMLPLGVVLTIPAAGSESSGGSVITNEDGLYK
                     RSVNSVNMRPKFAIMNPELTFTLPSYQTAVGAVDIMSHVMERYFTNVKNVDFTDRLCE
                     ATLKTMIVNTPIALKEPDNYEARAEIMWASTIAHNDLLGTGRIGDWATHGIEHELSAI
                     YDIAHGGGLAIMWPAWMKYVYKHDIERFAQFACRVWNVEPDFRNPEKTALKGIESLKG
                     FFSSLGIPVTLTDAGIPSDRLEEMARKATETGPLGNFVRLKKEDVEKIYRLAL"
BASE COUNT          663 a          669 c          746 g          564 t
ORIGIN      
        1 cccgatctcc tcgatcgaaa agtacctgct ccatctgttc aattcccggc cttccagcag
       61 gatcagcacc gtaggcacgg tgaaaaccga gtactccccg gccgcttcgg gatgcttctt
      121 tgtatcgaca ctaacaaact cccactgagg gaaatccgcc tctaaactct cgcgccttct
      181 tttcacggcg gcgcagacac cgcaggcgtt gctgaaaaaa tccaccagca tctttccgcc
      241 gagtcctccg agcgccccga ctgttagctc cttcaagttc tcacctcccg ttagtattat
      301 aattgaacaa aagagatcgg acttagaggt ggagtctttg gaaagtacca cggcagttct
      361 cgtaccggtt ttaagaatat gcggcgatga ccatcttatc ctcaccagaa ggtcgcgaaa
      421 gataagccat cccggccaga tgagcttccc cggcggtcac cgcgagaatg gggaaacgct
      481 tctcgagtgt gcggtgagag agatggaaga agagataggc gcctctccgt cacggatcga
      541 agaggtctgt ccgctgggtg tcacaacgac ggtcacgtcc ggcaaggtga tcgttccctt
      601 catcgggttc attgatcgtc cctttttcag gttgaacaga tcggaggttg aagatcttgt
      661 ctttctctct ataccggcgc tgaaatatgt atcgccggaa cctatcgtca tgccgagcgg
      721 aaaagtgacg atcaggtaca ggttccccgg cttcatcgtc tggggggcga cggcgcggat
      781 aatcgagagc tcgctgaaca ggatagtcga actcatagac cggcggaaca ggggaatgcc
      841 gctttgcaac gatggactta ttgataataa aaaggagtga agagatgctt gatttcgtat
      901 tcagcaacgc aaccaaaata atcttcggaa gaggaacgga actgaaaacc ggccaggaga
      961 tatcgcgcta cggaaagaag attctgctcc actacggcgg gggtagcata aagaagtccg
     1021 gcctgtacga cagggtaatc gagagccttc gcaaagccgg tgtcgaggcg atagagcttg
     1081 gcggggcaat gcccaacccg aggctttcgc tcgtcaggga aggcatagaa ctgtgcagaa
     1141 agcacgggat agacgccatt ctcgcggtgg gcggcggaag cgtcatcgat tcggccaaag
     1201 cgataggcat aggcgttccc tacgcaggag acgtctggga cttctattcg accggcagga
     1261 aggtcgagga gatgcttccg ctgggagtcg ttttgacgat accggcggcc ggcagcgagt
     1321 cgagcggcgg ttcggtcatt accaacgaag atggtcttta caagcgctcc gtgaacagtg
     1381 tgaacatgcg accgaaattc gcgataatga accccgaact gaccttcaca cttccatcct
     1441 accagacggc cgtcggcgcg gttgacataa tgtcgcacgt aatggagagg tacttcacca
     1501 acgtcaaaaa cgtcgacttc accgatagat tgtgcgaggc aacgttgaaa acgatgatag
     1561 tcaacacgcc gatcgccctg aaagaacccg acaattacga ggccagggcc gagataatgt
     1621 gggcctcgac gatagcccac aacgaccttc tcggaaccgg aagaatcggc gactgggcga
     1681 cgcacggcat agagcacgaa ttgagcgcta tctacgacat agcgcacggc ggaggcctgg
     1741 cgataatgtg gcccgcgtgg atgaagtatg tctataagca cgatatagaa aggttcgcgc
     1801 agttcgcctg cagagtctgg aacgtggaac cggatttcag gaaccccgaa aagacggctc
     1861 tcaagggcat agagagtctg aaaggcttct tctcgagtct tggcattcct gtaacgctca
     1921 ccgacgcagg cattccgtcg gacaggctcg aagaaatggc gcgaaaggcc accgaaacag
     1981 gcccgctcgg gaatttcgtt agactcaaaa aagaagacgt ggagaagatt tacaggctgg
     2041 cgctttgaat ctgtaaagag cgttaggagc cgcggtgagt agtggatagt gggtagtggt
     2101 gggggcaaga gctatggaca gattttcgcg atggacataa agcgcgaaga acgattcatc
     2161 gttccgacgc tgcgcgtcca ggtttttggt aaccctgagg tcagaggcca gaggtgagag
     2221 gtgtgaagag cgggaagatc ttgattttct aacctctaac ctcatgcctc ttatctcttt
     2281 tcgaaaatgg ggactgaccg gctcctgagg tctgtaaccg attttcgcaa gcctcggctc
     2341 ctatgctgtc atcccgtgtc atcccgtagt gctcttatac gggatctcgt tcttctcgtc
     2401 agtctctccc tcgcgattta atgtgttatg atgtaggtgg agttactcca cccgggtgta
     2461 gtaactccac tcattcaaag cgaggtgaac acatgctttt ctccatcagc ccgaagacaa
     2521 aacggagcga gctgttcgac cgcgaaagag aaatgaatga attgaaaagg gccgtgggca
     2581 acgatcgtct gatcgtactc gacggtatag ttggctacct cgcaattttc ggatatgaag
     2641 tc
//
LOCUS       DAOOLY010000308         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_48523_length_2610_cov_2.882192, whole genome shotgun sequence.
ACCESSION   DAOOLY010000308 DAOOLY010000000
VERSION     DAOOLY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48523_length_2610_cov_2.882192"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLD15_11460"
     CDS             <1..622
                     /locus_tag="PLD15_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64574.1"
                     /translation="TNLSTFTGNRFAIAPSTNPRIWMDIIRNVLWTAVDSNTWRTSFN
                     IAVTNNTAAPGTYVLNNEVDIYDEDQVFDEDSASITITVRGGTWGEETAWGGNYPGGG
                     SSWWYYFDTNGPSVQDIYAGQNLIQGASVKYDKAMGKLIITLGPNMRLQSVSDPVKVQ
                     GYSSIPSSRPEPGTFTTYKGTSLVISVPYYRYFAIHLDVEVWSPSK"
     gene            843..2084
                     /locus_tag="PLD15_11465"
     CDS             843..2084
                     /locus_tag="PLD15_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64575.1"
                     /translation="MRRTALLILAVLFSVAAFGQLGLRTVTSTATETETSVATSAIST
                     TGTLSLRTVDPLLLSFREGFKAPDDPGWIEKKVGNVSLKLPGLFSVSSQKFGSNYEAQ
                     IFDGNGKLFGRFFYYQLEPYNLEELLNAVIPSVYGNTTTGEKSYEETMWMENGMGVYL
                     ASLKMVANIDFPVIFVYTPGDEITVTSQGPVAMFFFEPVEYTGEKLDEAKAIIADIVG
                     SYLFPIEEKEEDEGGIKPPKVIEERDPFVRLFMDLFNDSRSPVNTDDWIEVQGDRFGF
                     MMNPQFSVHFYPGETSETADLGYSGTVVSKIVVGEIDTSSPIEGVFKEVIEEFLAVLA
                     VEGYEIVDSFSSVVKEGTAVNIYAVDFAGVLCWIIIYSETDSIASFGPGDYFIFIGLP
                     DSDNLSQWAEWYAGMVISLDF"
     gene            complement(2174..>2610)
                     /locus_tag="PLD15_11470"
     CDS             complement(2174..>2610)
                     /locus_tag="PLD15_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64576.1"
                     /translation="NSIKNGQSLLYWNDNGDVLANYSLSYLFLQYVRTQGGGDPEIFK
                     DILLNSKNDQQAIIDSLSKRGLSIDFGNLMTSFRLALLLKDPSGLYGFNGDSDFDGIN
                     ASYYIGGAKNIRGGSAFFKTISESFTDPENAGNTIQYVGITN"
BASE COUNT          704 a          563 c          634 g          709 t
ORIGIN      
        1 cacaaacctc tccacattca cgggtaacag attcgcgata gcgccctcta ccaatcctag
       61 aatctggatg gacattataa gaaacgttct gtggaccgct gttgattcga atacctggag
      121 aactagtttc aacattgcgg taaccaacaa tacagcagct ccggggactt acgttctcaa
      181 caacgaagta gatatttatg acgaagacca ggtattcgac gaagactccg catccataac
      241 cataaccgtg cgcggaggaa cctggggtga agaaaccgcc tgggggggaa attatcccgg
      301 cggtggatct tcctggtggt attacttcga tactaacgga ccttcagttc aggatatata
      361 cgcgggacag aatctgatac agggtgcatc tgtaaagtac gacaaggcta tgggtaaact
      421 gataataact ttagggccca atatgcgcct tcagagcgtg agcgatccag tcaaggtaca
      481 gggctattct tccataccta gcagcagacc agaaccaggt acatttacaa cctacaaggg
      541 aacaagtctt gtgatatctg tgccttacta caggtacttc gcgatccatc tcgatgtaga
      601 agtatggagt ccatcaaagt aatagctgaa taaactttgc agttccccca taaagccctc
      661 cgaatggagg gcttttcttt tacgaatcga tactgggact tagaaatttg gctggataca
      721 ggaacttctc aatattcatt gttcattcac tcgactgttt gcatgaaaaa gaggggtacg
      781 ggagtagaat tcatttggaa tggttctcgg cacacaatgt gctgactgtg gaggtgaaag
      841 cgatgaggag aacggctcta ttgatactcg cagtcctgtt ctctgtagct gccttcggac
      901 agcttggctt gaggactgtg acatcgacgg caacggaaac ggaaacaagc gtagcaactt
      961 ctgcaatctc aacgaccgga acactgagct taaggacggt tgatccactt cttctctctt
     1021 tcagagaagg cttcaaagca ccggatgatc cgggctggat cgagaaaaag gtaggaaatg
     1081 tctctttgaa actgcccggt ttgttcagtg tttcttcaca gaagttcggt tcgaattacg
     1141 aagcacagat cttcgacggt aacggaaagc tttttggtcg cttcttttat taccagcttg
     1201 aaccttacaa tctcgaagag cttttaaatg cagttatccc ctctgtttac gggaatacca
     1261 ctaccggcga gaagagttat gaagagacaa tgtggatgga gaatggcatg ggggtatacc
     1321 tggcatccct gaagatggtt gccaacatcg attttcctgt aatattcgtc tatactcccg
     1381 gagatgagat cactgttacc agtcagggac ctgtggccat gttttttttc gaaccggtcg
     1441 agtacaccgg tgaaaagctg gatgaagcaa aagcgataat agccgatatt gtcggtagtt
     1501 atttgttccc gatcgaagag aaggaagaag atgaaggcgg tatcaaaccg ccaaaagtaa
     1561 tcgaagagcg agatcccttc gtcaggttgt tcatggattt attcaatgac agcagatctc
     1621 ctgtaaatac ggatgactgg attgaagtgc agggagaccg tttcggtttc atgatgaacc
     1681 cacagttctc ggttcacttt tacccgggag aaacttctga aaccgcagat ctgggatact
     1741 ccggcactgt ggtctccaag atcgtagtag gagagattga tacatcttca ccgatagagg
     1801 gtgttttcaa agaagtaata gaggagtttt tggccgtatt agccgtagag ggctatgaga
     1861 tagtagatag tttttcctct gtggttaagg aaggaaccgc cgtcaatatc tacgcagtcg
     1921 atttcgccgg tgttttgtgc tggatcatta tctattccga aaccgattcg atagcgagct
     1981 ttggacccgg tgattatttc atctttatcg gcctgcctga ttcagataat ctttctcaat
     2041 gggcggagtg gtacgcaggg atggttatat ctttagattt ctaaagaagc ttaaaccgac
     2101 atacaaaaaa gccccggagg actggacgat tatcggccgt gactccgggg tctttgctgg
     2161 tgaattgacc tcttcaattg gttatgccaa cgtattgaat cgtgtttccg gcgttctccg
     2221 gatctgtgaa agattcgctt atggttttga agaacgcact gccacccctt atattttttg
     2281 ctccgcctat ataataagat gcgtttattc catcaaaatc actatctccg ttgaagccat
     2341 acaatcctga cgggtccttc aatagcaacg ccagtctgaa cgatgtcata agatttccaa
     2401 agtcaatgct caagcctctc tttgaaagag aatcgatgat agcctgctga tcgtttttgc
     2461 tgttcaacaa tatgtctttg aatatctcgg gatcgcctcc accctgtgtt cttacgtact
     2521 gaaggaagag ataagagaga gagtagttgg ccaggacatc gccgttgtcg ttccagtata
     2581 aaagtgattg gccattcttt atgctgttcg
//
LOCUS       DAOOLY010000309         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_48609_length_2606_cov_4.678165, whole genome shotgun sequence.
ACCESSION   DAOOLY010000309 DAOOLY010000000
VERSION     DAOOLY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48609_length_2606_cov_4.678165"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLD15_11475"
     CDS             complement(<1..292)
                     /locus_tag="PLD15_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64577.1"
                     /translation="MIVSIDKLRKGFASKPVLNDISFNVEQGDIFAIVGPNGAGKTTT
                     LRCIFGELAPDGGKVAVFGERVTSRTKEKIAVMTEDRLTFRRFTGDDYLRMWR"
     gene            complement(289..870)
                     /locus_tag="PLD15_11480"
     CDS             complement(289..870)
                     /locus_tag="PLD15_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64578.1"
                     /translation="MTEKIEAYFMKELSEKAGRIAVFSLGLLIPFYPARMVFLFLLAT
                     SLLPSDIQHKRLGSLLALPYSRQELFWISYLFLIAFGTVAQVIGMALFGAQFGSSTSI
                     YWLSMLIGSISFSTAYYAVSMISVTAGLDNFGIPFLVLIADLIIGGFGNRWNNPYFYA
                     SPAHQGNVWVSGAVSLAVLLVANYLFVKKGVQK"
     gene            complement(863..1219)
                     /locus_tag="PLD15_11485"
     CDS             complement(863..1219)
                     /locus_tag="PLD15_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPB64579.1"
                     /translation="MFGKIDKHDGVPAYIQIMNVVKKEIMLGRLENGDQLPPVREMAV
                     MFGVNTNTVIRSLERLQFEGLVEAEHGVGYFVKTAQAVNREVIKTISETVEELKKSGI
                     DLQMAKVLLEEVWKND"
     gene            complement(1233..2270)
                     /locus_tag="PLD15_11490"
     CDS             complement(1233..2270)
                     /locus_tag="PLD15_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64580.1"
                     /translation="MNRKYPSIAKLILAGGLGLLALLIPFVAVKILLAIAGIVVIALS
                     LKKFKWLLITLGVLFLVIPVVALSVISGIGNNILRWPLGFIDDPSILRWFGWIENDGY
                     EYHGDGGNDYNRKSSDHRTYMPDLYVEGAKEIVITGLGYEVSFNENSDQVYIPSELEI
                     QRDGERLLVSMPDYMNNSTAHITVGTLVQLEKVEFRTAYLRLNDRVKVESVEIETTAG
                     EIRGEITASEKISIESTSLSITGSLKSPEIVIANVPALSFTGSVEAGEFLVRNGVALS
                     FSMEASKIEDLSIEGAVINGRIKFNDSWNGRRRVKVSAFSGSLNLMMPAGSGGFELVT
                     SGMVSVVTEKY"
BASE COUNT          654 a          732 c          563 g          657 t
ORIGIN      
        1 ttctccacat cctgaggtaa tcatcgcccg tgaatctcct gaaagtcagc cggtcttcgg
       61 tcataacggc tatcttctct ttggtgcgcg atgtaaccct ctccccgaac acagctacct
      121 ttcccccatc tggagccagc tcaccgaata tgcatcggag agtggtggtc tttccggctc
      181 cgttgggtcc gactatcgcg aagatgtcac cttgctcgac gttgaaagag atatcgttca
      241 gtacgggttt cgacgcgaaa ccctttctca gcttatctat ggagactatc atttctgcac
      301 tcctttcttg acgaagagat aattcgcaac aagtaacacc gccagcgaaa ccgctccgct
      361 gacccatacg ttgccctggt gagccgggct tgcatagaag tacgggttgt tccacctgtt
      421 tccgaaacct cctattatca gatcggcgat gagcacgagg aagggaatac caaaattatc
      481 caggccggcc gtaacactta tcatcgaaac cgcgtaatag gcggtcgaaa aacttatcga
      541 accaataagc attgagagcc agtaaatact ggtcgatgag ccgaactgcg ctccgaacaa
      601 cgccatcccg atcacctggg ccacggtacc gaaggcgatg agaaagaggt acgatatcca
      661 gaagagctcc tggcgcgaat aaggcagagc cagaagcgag cccagcctct tgtgctgtat
      721 atccgaaggg agaaggctcg tggcgagcaa aaagaggaag accattctcg ccggataaaa
      781 gggaatcagc aatcccagtg aaaagacggc tatccttcct gccttttcgc tcaactcctt
      841 cataaaatag gcttctatct tttcagtcat ttttccatac ctcctccagc agaactttcg
      901 ccatctgtag atctatgccg ctcttcttca attcttcaac ggtttccgat atcgtcttta
      961 taacttctct gttgaccgcc tgcgcggttt tcacaaaata gcctacccca tgttccgcct
     1021 ccaccaaccc ttcgaattgc agcctttcga gtgatctaat caccgtgttc gtattcactc
     1081 cgaacatcac ggccatctcc ctcaccgggg gaagctggtc gccattttcg agacgcccga
     1141 gcattatctc tttttttacc acattcatga tctgtatata ggccggaacg ccatcgtgtt
     1201 tatcgatttt tccaaacatt ccatcacttc tttcaatact tctcggtaac tacgctcacc
     1261 atacccgagg taaccagttc aaaacctccg ctgccggcgg gcatcatgag attgagcgat
     1321 ccactgaagg ccgaaacctt cacgcggcgt ctcccgttcc acgaatcgtt gaatttaatc
     1381 ctgccgttga taacggctcc ttcgatcgaa agatcctcga tttttgaggc ctccatagag
     1441 aacgacagcg cgactccgtt cctgaccagg aactcaccgg cctcgacgct ccctgtgaag
     1501 gaaagcgccg gaacgttcgc gataactatc tccggtgatt tgagcgatcc ggttatagat
     1561 agagaggtac tttcaatcga aatcttctcg ctggccgtta tctctcccct gatctctccg
     1621 gccgtcgtct cgatctctac actctcgacc ttcaccctgt cgttcaacct caagtaggcc
     1681 gtcctgaact ccactttctc gagttgaacg agtgtaccca cagttatatg ggccgttgag
     1741 ttgttcatat aatcaggcat cgataccagc aacctctctc catctctctg aatctctagt
     1801 tccgaaggaa tatagacctg atcggaattc tcgttgaaac tcacctcgta acccagaccg
     1861 gttatcacga tttccttagc tccttccaca tacagatcgg gcatataagt cctatgatct
     1921 gagctcttcc tgttgtagtc gtttccgccg tccccgtgat actcataacc gtcgttttcg
     1981 atccagccaa accaccgcag aatcgatgga tcgtctatga atcccagtgg ccagcggagt
     2041 atattgttac cgattccact tatcacgctc agagctacga cgggtataac caggaagagt
     2101 acgccaagtg ttatcaacaa ccatttaaat ttcttcaggc tgagagcgat gacaacgatc
     2161 ccggcgatcg ccaggagaat cttgaccgcc acgaatggaa tcagcaaagc cagtaaaccc
     2221 agtccccccg ccagaatcaa tttagcgatc gaaggatact ttctattcac ctgcacacct
     2281 ccataatcat atagtgttct agtatattag aacactagtt atagatgctg tcaagtacta
     2341 ttttcatagc cgtccttcga gagggagaga gaagagagaa gagaggagga gagaaggaga
     2401 gacgccttat cgtgagcgaa gcgaactctc gatgctcttt ctctcgtgtt tttaccaaga
     2461 acttacaaag cacaaccaac aactgttcct atagggaccg gaggtgactg gcattgcgaa
     2521 gcgattatga ttcaggaaag tctgtttcct ctagaaggtg tatccctctt caatgagctc
     2581 tatgaggggc cgaaaccaca gccggg
//
LOCUS       DAOOLY010000310         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_48763_length_2601_cov_4.351532, whole genome shotgun sequence.
ACCESSION   DAOOLY010000310 DAOOLY010000000
VERSION     DAOOLY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48763_length_2601_cov_4.351532"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="PLD15_11495"
     CDS             <1..1774
                     /locus_tag="PLD15_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPB64581.1"
                     /translation="TIPEGEERYLHVYDQYKVQRSRGYAPVPFILSDRGRALLIDTGF
                     LSSFKIDDSKITLSIYTRGCSIEGTEIRFWKEDSPLKAIEKIYKIAKPCPPPLWALGP
                     WLSANQWNSQKKVMEVLRETVERDLPATTLVVEAWADEQTFYIFNGAEYDPVSGDDKF
                     NLKDFRFHEPWPDPVEMINKLHERGIRLVLWQIPVIKCFDESTPQPAIDIRYGSSRGY
                     FARTGDGSDYRIPVARWHEGNVVVDFYNEEAAKWWASKREYLVEELGVDGFKTDGGEH
                     LWGRDTLTAAGSAAKVRNTYPERYFETIAGILREDGILFSRAGYTRSPAHTLFWVGDE
                     DSTWEALRDNITAGLNVSISGNPFWGWDIAGFSGEVPSMELYRRSIELAVFTPVFQLH
                     SEDSGDPSPSSERTPWNLAKAWEDESIIDYYRNFASLRMTLSPYIYRESLHAAQAGLP
                     LTLPLLLIEKENDPEGLTYMFGRDMVVSPAVDEEMKERKISLPEGHWVNLWNGRTHSG
                     QIAIDGERTEVFLRKGSVIPLSIPPDGTLFGTNWSQEETGLLYVGSEFPENLGVRLSS
                     LARRLARISGVESGIIKIEWREIR"
     gene            1756..>2601
                     /locus_tag="PLD15_11500"
     CDS             1756..>2601
                     /locus_tag="PLD15_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPB64582.1"
                     /translation="MERNQIMDLLERSTAIIEANQSKHGSYIASPSFSQYGYCWFRDG
                     SFVAHAMEKAGRKESSARFIRWGLAVLKRYRDSLQKATVGSVTLDSFLPTRYTMEGFY
                     SEDNWTNAQSDGYGTFLWVVAERPELLDQSDCEICDLCARYLERVWRLPCYDVWEESP
                     TMVHTSTLLSIVAGLKAIEGIINRKTAWRDIMDFILEELTSDGRLKKSNAADEVDSSL
                     IWASYPYGLLDGDSLLMKKTARTVMNELYFDGGLKRYRKDTYFGGGSWILLSAYLAGH
                     LKSNNE"
BASE COUNT          699 a          636 c          705 g          561 t
ORIGIN      
        1 aaccataccc gaaggcgagg agaggtatct ccacgtttac gaccagtaca aggtccagag
       61 atccagagga tacgctccgg taccttttat cctttcagac aggggaagag cacttcttat
      121 agacactggc tttctctctt cgttcaaaat agatgattcg aagataacgc taagtatcta
      181 cacaaggggc tgttcgattg aaggtacgga aatccggttc tggaaagaag actctcctct
      241 gaaagcgatc gaaaagattt acaaaatcgc caaaccctgc ccgccaccgc tctgggcgct
      301 cggcccctgg ctatctgcca accagtggaa cagtcagaag aaagtcatgg aagttctcag
      361 ggaaacggtc gagagagatc tacccgcaac cacgctcgtt gttgaagcct gggccgacga
      421 gcagactttc tatattttca acggtgcgga gtacgatccc gtttccggcg acgacaagtt
      481 caatctgaaa gacttcagat tccatgaacc gtggcccgat cctgtggaga tgatcaacaa
      541 gcttcatgag agaggtataa ggctggtgct atggcagatc cccgttatta agtgttttga
      601 cgaatcgacg cctcagccgg caatagatat ccgttacggt agttcgagag gatactttgc
      661 aagaaccgga gacggttccg attacaggat tccggtggcc cgctggcacg aaggcaacgt
      721 agtagttgat ttctacaacg aagaagcggc taaatggtgg gcttcaaaga gggagtacct
      781 cgtcgaagaa cttggagtcg atggcttcaa gaccgacggc ggcgaacacc tttgggggag
      841 ggacactctt acggccgccg gaagcgctgc gaaagttaga aacacctatc ccgaaagata
      901 cttcgagact atcgccggca tactgagaga agacgggatt ctcttcagcc gcgccggcta
      961 caccaggtcc ccggcgcata ctcttttctg ggtaggcgat gaggattcga cctgggaggc
     1021 ccttcgcgac aacataacgg ccggactcaa tgtctctatt tcaggcaacc ccttctgggg
     1081 atgggatata gccggtttca gcggagaggt cccttctatg gagctgtaca gaagatcgat
     1141 agagctggcc gtcttcacgc ctgtctttca gcttcattcg gaggattccg gcgatccctc
     1201 gccctcttcc gagaggactc catggaacct ggcaaaggcc tgggaagacg aaagtatcat
     1261 tgattattac agaaacttcg cctccctgag aatgacactc tcgccgtata tctacagaga
     1321 gtctctccac gctgcacagg caggtcttcc cttaacgctg ccgctgctgc tgatagagaa
     1381 agagaatgat ccggagggcc tcacatatat gtttggaaga gatatggttg tctcgcccgc
     1441 cgttgacgaa gagatgaaag agagaaagat ctcccttcct gaaggtcact gggtgaatct
     1501 ctggaacggc cgaactcatt cagggcagat cgcaatagat ggagagagaa ctgaagtttt
     1561 cctcagaaag ggatcggtaa tacccctcag catcccaccc gacggcactc tcttcggaac
     1621 gaactggtca caggaagaga cgggactgtt gtacgtcgga agcgaatttc cagaaaatct
     1681 cggagtgcga ctaagcagcc tggcgagacg actggcaaga atatccggcg ttgaatcggg
     1741 aataataaaa atagagtgga gagaaatcag ataatggact tactggaaag atcgacagcg
     1801 ataattgaag ccaatcaaag caaacacgga agttacatag cctcgcccag cttctcgcag
     1861 tacggttact gctggttcag agacggctcc ttcgtggcgc acgcgatgga gaaagccgga
     1921 aggaaagaga gttcggcccg gttcataaga tggggtctgg ccgttcttaa aagataccgc
     1981 gattctctcc agaaggctac cgtggggagc gttacactgg acagcttctt accgacaaga
     2041 tacaccatgg agggatttta cagcgaagat aactggacaa acgcccagag tgacggttac
     2101 ggcacttttc tatgggtcgt tgccgagagg cccgaactcc tggatcagag cgattgcgaa
     2161 atctgcgatc tatgcgcaag gtatctcgaa cgagtgtgga gactcccgtg ctatgatgtc
     2221 tgggaagaat ctcccaccat ggtacatacc tctacgctgc tctccatagt tgcgggattg
     2281 aaggcgatcg aaggtatcat aaacaggaag accgcctgga gagacatcat ggattttata
     2341 ctggaagagc tgacgagcga cggccggctc aagaaatcga acgctgccga tgaagtcgat
     2401 tcgagtctta tctgggcatc ctatccttat ggacttcttg acggcgattc acttctcatg
     2461 aaaaagacgg cacgaaccgt gatgaacgaa ctctacttcg acggaggcct caagaggtac
     2521 agaaaagata catatttcgg tggcggtagc tggatactgc tgagcgctta tctagccggt
     2581 catctgaaat cgaacaacga a
//
LOCUS       DAOOLY010000311         2599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_48813_length_2599_cov_3.982311, whole genome shotgun sequence.
ACCESSION   DAOOLY010000311 DAOOLY010000000
VERSION     DAOOLY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48813_length_2599_cov_3.982311"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLD15_11505"
     CDS             <1..215
                     /locus_tag="PLD15_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPB64583.1"
                     /translation="VLVLTPDDNTNLMVAMSAKEYFKVTRVVARAYDPNNIEMFSDFG
                     IEVICPTLLAVENIKNVLYFIGGDER"
     gene            212..871
                     /locus_tag="PLD15_11510"
     CDS             212..871
                     /locus_tag="PLD15_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPB64584.1"
                     /translation="MKIVLIGGEKIAYHLARRFLSKGYQVMVVNKNEKFCEEIAKKLR
                     ALVIRGDGSRRYVLEQLELDRTDLFVALTPNDQDNLVACLTAARVYGIERPVALVNDP
                     DNKATFQMMGVKNVVSPTEILGSALEDSLFREQITNLLPSSEKISILRIEMTDKSPAT
                     GQAVKNLPLPKESVIGAIIRENEVIIPRGDNVIEEGDILLVLSNPKVQSSVFEVLLGE
                     V"
     gene            872..2356
                     /locus_tag="PLD15_11515"
     CDS             872..2356
                     /locus_tag="PLD15_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPB64585.1"
                     /translation="MYRNLLKQRYRLVFGYIGNLFVFFPLVLLLPMLYCIWDPSVFME
                     SLPFLEAAVISFSFGMLFKLIMKVRPGAPITVQEGAIIVFFAWVCGIVFSALPFIFGG
                     YLNFTQAIFESTSGLTTTGLTMVDVLTIPDTYLLWRSILQFIGGAGFAIIMMSAILGP
                     TGLGLYKAEGRMDNLVPNIKSSTRTIAVIYTTYAVAGILMYKIAGMNWFDALNHSLTA
                     LATGGFSTRLGSIGEFNSLPVEIVTIVLMLLGATGFGIHYTLWRRNFRAFWKNGEPKL
                     MFLMIAFFTPLIMAGTLKYFYESIGTQFRYALFQSVSALTGTGFSTVDFIPWNHMGLY
                     LITLLMIFGGDLDSTSGGLKQYRLFALGKLLWMEIKMYFLPKNALLKEEIWKGETKRY
                     LDHSLVKEILLIFSLYFITFAVGVGITASYGHSLAFSAFEFASALSTVGLSVGITLPS
                     SPAGLIWTETVGMFLGRLEFLVVIYAITKLLRDGKVMLAKRRND"
     gene            2360..>2599
                     /locus_tag="PLD15_11520"
     CDS             2360..>2599
                     /locus_tag="PLD15_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPB64586.1"
                     /translation="MINRIKGTQDIAFEDIEYWQYIEQAVRNITFRYAYNEIRTPIFE
                     ATELFKRSVGESTDVVQKEMYTFDDKGGRSITLRPE"
BASE COUNT          675 a          578 c          672 g          674 t
ORIGIN      
        1 ttgtactggt cctgacgccc gatgataata cgaacctgat ggtcgccatg tcggcgaaag
       61 agtattttaa agtgacgcgt gtcgttgcga gagcctacga tccgaacaac atagagatgt
      121 tttcggattt cggaatagag gtcatctgtc ccaccttgct cgccgtagaa aacataaaaa
      181 acgttctcta cttcatcggt ggtgacgaga ggtgaagata gtccttatag gtggcgaaaa
      241 aatagcctac cacctcgcca gaaggtttct gagcaagggt taccaggtta tggtcgtaaa
      301 taaaaacgag aagttctgcg aagagatagc caagaaactc agagcgctgg ttataagagg
      361 cgatggaagc cgtaggtatg ttcttgaaca gctggagcta gatagaaccg atctcttcgt
      421 tgcgctgact cccaacgatc aggacaatct ggtcgcatgt ttgacggcgg cgagagtgta
      481 tggtatagag aggcctgtag ccctggtgaa cgatcccgat aataaggcga cttttcagat
      541 gatgggagtt aagaatgtcg tgagtcccac ggagatactg ggatcggcgc tcgaagacag
      601 tcttttcaga gagcagatca ccaatcttct accgtcgtcg gaaaaaatct ccatattgag
      661 gatcgagatg accgataagt ctccggcaac aggtcaggcc gttaagaatc ttcctctccc
      721 gaaagagagc gtgataggtg cgattataag ggagaacgag gtgataatcc caagaggaga
      781 caacgtcata gaagaaggcg atatcctcct ggttctcagt aatccaaagg ttcagtcatc
      841 ggttttcgaa gtgcttctgg gagaggtata aatgtaccgc aatcttttga agcaacgcta
      901 cagactggtt tttggctaca tagggaatct tttcgtcttc ttcccactgg tgttgctcct
      961 tccaatgttg tactgtatct gggatccttc ggtatttatg gagagtctgc cgtttctaga
     1021 agcggcggtg atcagcttct ctttcggcat gttattcaag ctcatcatga aggtcaggcc
     1081 cggggcacca atcacggttc aggaaggcgc tatcatcgtg tttttcgcat gggtatgcgg
     1141 aatagtcttt tcagcgcttc ccttcatctt tggcggttat ctcaacttca cacaggcgat
     1201 tttcgagtcc accagtggat tgacaactac cggacttacc atggtagatg tgctcacgat
     1261 acccgacact taccttttgt ggcgaagcat attgcagttc attggaggag cgggcttcgc
     1321 gatcataatg atgtccgcta tacttggacc gacaggactc ggcctttaca aggccgaagg
     1381 gcgcatggac aacctggtgc ctaatataaa aagctcaacc agaacgattg cggttatcta
     1441 cactacatac gctgtggcag gtattttgat gtacaaaata gcaggcatga actggttcga
     1501 tgccttgaac cactctttaa cagccctggc taccgggggt ttttcgacca ggcttggctc
     1561 tatcggagag ttcaacagtc tgcctgtaga gatcgtgacc atcgtcctaa tgctgctggg
     1621 cgctaccggt ttcggcatcc actacacact ctggagaaga aatttcagag ccttctggaa
     1681 aaacggagag ccgaaactga tgtttctgat gatcgccttc tttactcctt tgataatggc
     1741 cggaacgctg aaatacttct atgaatcgat aggaactcag ttcagatacg ctctctttca
     1801 atccgtctcg gcactgacag gtaccggttt ttccactgtt gactttatac cctggaatca
     1861 catgggactc tacctgataa cacttctgat gatattcggg ggagatctcg attctacgtc
     1921 gggaggattg aaacaataca ggctctttgc tctgggaaaa ctgctctgga tggagataaa
     1981 gatgtatttt ctgccgaaga acgcgttgct gaaagaggag atctggaagg gcgagactaa
     2041 aagatatttg gatcattcgc tggtgaaaga gatactgctg atcttctcgc tttacttcat
     2101 cacgttcgcc gttggagttg gcattacggc atcttacggc cactcgctgg cattctccgc
     2161 ctttgagttc gcctccgcac tcagtacggt cgggctgtcg gtgggaatta cccttccctc
     2221 ttcacccgca ggtttgatct ggacggagac cgtcggtatg tttcttggaa gacttgagtt
     2281 tcttgttgtg atatatgcta taactaaatt gctgagagac gggaaagtaa tgctggctaa
     2341 acggaggaat gattgaggaa tgattaacag aatcaaggga actcaagaca ttgctttcga
     2401 agatatcgaa tactggcagt acatagaaca ggcggtgcga aacataactt tcaggtacgc
     2461 ctacaacgag atacgcacgc cgatattcga agcaaccgag ctgttcaaaa gaagcgttgg
     2521 agaatcgacc gatgtcgttc agaaggagat gtacactttc gacgacaaag gcggaaggtc
     2581 gatcacactc agaccggag
//
LOCUS       DAOOLY010000312         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_48866_length_2597_cov_5.366247, whole genome shotgun sequence.
ACCESSION   DAOOLY010000312 DAOOLY010000000
VERSION     DAOOLY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48866_length_2597_cov_5.366247"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1735)
                     /gene="mgtE"
                     /locus_tag="PLD15_11525"
     CDS             complement(392..1735)
                     /gene="mgtE"
                     /locus_tag="PLD15_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPB64587.1"
                     /translation="MKTELYEIVEEFIEKRDWQSIRRIISEESPPEIAELIMDLEKKS
                     RVILFRIIPKELAAEAFSYLESRDQDLLLGDLTDQETKALLEELSPDDRTEILGELPG
                     QATQRLLNLLSPKDLDEARTLLGYPPESVGRLMTPDYVAVRPEWSIEKSIEHIRKRGR
                     DSETVNMIYVTDHSWRLLDSLELRRFILADPGITVKDIMDYRFTAISPFADREEAVRL
                     IQMYDLVAIPVIDSAGVLLGIVTVDDILDIAEEEVTEDFQKTAAVGPLKTSYAYASFW
                     TLYSKRILWLMILILVNLVSSSIIANFEATLASTIALAFFIPLLIDSGGNAGAQSATL
                     MVRAIATGDVKMTRWMATFLKEILVGISLGITMGLASSLLGLFRGGLEIGLVVGISMT
                     AIVLVANLIGAMVPFVLTWFKLDPAVASSPLITTIVDAVGLLIYFTIANAIIGSM"
     gene            2055..>2597
                     /locus_tag="PLD15_11530"
     CDS             2055..>2597
                     /locus_tag="PLD15_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB64588.1"
                     /translation="MEDKRKLLYDCAKELFGVKGFKDTNISEITQKAGVAVGTFYKYY
                     PSKEKLFMDIFLDENVRLKKRFLQSLDLTRSPLEIMRQMMALNAEGMNANPILREWYN
                     KSVFEKIEKLYREENGIQVVDFLYDTFLELVNKWQAQGKMRKDIDSKMIMMVFAAIIN
                     IDTHKDEIGIEYFPQLLALIA"
BASE COUNT          754 a          610 c          568 g          665 t
ORIGIN      
        1 ctcatgatcg aaaactacag aagcggaatg atctgggaac tgaccatggg tagccaattc
       61 ataaaaaccg gcctccaaag gctgggcttc tcgcagcggt aagagccgca agagccgtcc
      121 ttggtccggc gtcccggagc atgaacccgt ccttcggaag agcgagaaga gcggttcctc
      181 gatattacgc atccagaatg cccccacact gtcatcccgt ttggcgaaaa tggggacggg
      241 ttcctggcgt ctgtacgcaa ttttcgcatt tactaactca tgtagaaaaa tggtgtctat
      301 ttttttctgg tggaggttct tcctcagggt atagcacttc aaaagctctc cagttttaca
      361 cccagagttc ccccgaaaga tgataatcct gtcacatgct accaataatt gcatttgcga
      421 tcgtgaaata tatcaataaa ccaacggcat cgactattgt cgttatcaaa ggactgctgg
      481 ccaccgccgg atcaagtttg aaccatgtca gtacaaacgg aaccatagct ccgataagat
      541 tagctaccag aactatcgcc gtcatagaga tgccaacaac aagaccaatt tcgagacctc
      601 ctctgaacaa acccaataag gaactagcaa ggcccatcgt tatacccagg gagattccaa
      661 cgagaatttc cttcaagaaa gtcgccatcc acctggtcat cttcacatct cccgttgcaa
      721 tagcacgcac catcagtgtt gccgattgag cgcctgcatt tccaccgctg tctattagaa
      781 gagggatgaa gaatgcaaga gcaatcgttg aagcaagagt cgcctcgaaa ttcgcaatta
      841 tgcttgacga tacaaggttt acgagtataa gaatcatcag ccacaagatt ctcttggagt
      901 acaaagtcca gaaactcgca taagcgtaac tcgttttcaa aggtccaaca gctgcggttt
      961 tctgaaaatc ctcggttact tcctcttcgg cgatatcgag aatgtcgtct accgtaacga
     1021 ttccaagaag aactccagct gagtcaatga caggaatagc caccagatca tacatctgga
     1081 taagtctcac ggcttcttct ctgtcggcaa acggagagat ggcggtaaaa cgatagtcca
     1141 taatatcctt gaccgtaata cctggatcag caagtataaa ccttcgcaat tcaagagaat
     1201 caagcaaacg ccacgaatga tcagtcacat atatcatgtt tacggtttca ctatctctcc
     1261 cacgcttgcg tatgtgttcg atggactttt cgatactcca ctcgggcctt accgcaacat
     1321 aatctggtgt cattagacgg ccgacactct cgggcggata gcctagcagc gtcctggctt
     1381 catcgagatc ctttgggctg agaagattta gaaggcgttg tgttgcctgg ccgggaagct
     1441 caccaagaat ctcggttctg tcgtcggggc taagctcttc aagtagcgcc tttgtttcct
     1501 gatcagtaag atctccaagt agaaggtctt gatcccgcga ctcaagatat gagaaggctt
     1561 ctgccgcaag ttccttcggg ataatcctga agagaatcac tctcgacttc ttctcgagat
     1621 ccataatgag ctcagcaatt tctggaggcg attcttcaga gataatcctt cttatagact
     1681 gccagtcacg cttttcgatg aactcttcaa ctatctcata gagctctgtt ttcatttttc
     1741 acctccggaa aaggtctgga agatattata tatcaagatt accagattca cccgcgttca
     1801 ccttcccagt atgaaaggct gaaacagact tatgattcca caggctggac aacatgtgga
     1861 tagtacccac tcatctgatc gacagctatg tacccgattt tcgcatttac caactcatgt
     1921 agaaaaatgg tgtttgtccc tattttccca cgggatataa agttcgtact ttctggaaaa
     1981 cccgttgact ttcggtggcg ggttgagtac aatatgaatg aatataaaat tactcattca
     2041 cggagatgct tacaatggaa gataagagaa agttgctata cgattgtgcg aaagaactgt
     2101 ttggcgtcaa aggcttcaaa gatacaaaca tatccgagat cacacagaag gccggagtgg
     2161 cagtaggaac gttctacaaa tactatccgt caaaggaaaa actcttcatg gatatctttc
     2221 tggatgaaaa cgtcaggcta aaaaaacgct ttttgcagtc tcttgattta acccggagtc
     2281 ctttggaaat catgaggcaa atgatggcgt tgaacgccga gggcatgaat gcaaacccca
     2341 ttctaaggga atggtataac aaaagtgttt ttgaaaaaat cgaaaagctt tatcgtgaag
     2401 aaaatggtat ccaggtggtt gattttctat acgatacctt cctggaactt gtcaacaagt
     2461 ggcaggcgca ggggaaaatg cgcaaagaca ttgacagcaa aatgatcatg atggtttttg
     2521 ctgcaataat caatatagat acgcacaagg acgaaatcgg aatcgaatat ttcccgcagc
     2581 ttctagcgct tatagcg
//
LOCUS       DAOOLY010000313         2588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_49095_length_2588_cov_4.270825, whole genome shotgun sequence.
ACCESSION   DAOOLY010000313 DAOOLY010000000
VERSION     DAOOLY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49095_length_2588_cov_4.270825"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLD15_11535"
     CDS             complement(<1..597)
                     /locus_tag="PLD15_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64589.1"
                     /translation="MLQKTFILIFDVILISVALTLIQTVSPWFSFLLTGLFLFPILRM
                     SGLIRDLDEREKAVDNLSSNIALAIVFLLAMFYTGMGYEIGTDCFISFILIPFAAKAI
                     FSAGLSLSRNQSISIIGRTVGLIFIGFAVLSHGLSVTTAIEIIPGVAILAATELSKRW
                     KYFGLSFLAFIVVATLVYWNNFDRVTMVTVYALFVIPFS"
     gene            complement(599..802)
                     /locus_tag="PLD15_11540"
     CDS             complement(599..802)
                     /locus_tag="PLD15_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPB64590.1"
                     /translation="MKNRLREFRFKNDEITQESLAELVGVSRQTIIAIEKGRFNPSVK
                     LALKIARVLRCRAEDLFIIEEGD"
     gene            complement(1018..2178)
                     /locus_tag="PLD15_11545"
     CDS             complement(1018..2178)
                     /locus_tag="PLD15_11545"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPB64591.1"
                     /translation="MKISKEAAKRFLLSYQNLLPPRRLSGGEGILAFVRKVGCLQFDP
                     LNIAGINTDLVLQSRVKNYRPDMLYRLLYEDRKLLDGWDKNMSIYPIEDWPYFQRFRE
                     DAKDHIRRSEHIDEILEQIRKDIEVRGPLSSQDFDFDKKVGWYWAPAKASRAALESMY
                     WWGELVVHHRAGTRKYYDLAGRHIPQDIFDRLDPNTTEEAYHRWHISRRIDSIGLFWS
                     RSGDAWLGIKGLNSAKRTAAIEEMVRSGELIELEIEGFDYPVYTSSVNRELLDRSHES
                     TGAPEASFIAPLDNLLWDRKFIKSLFQFEYTWEVYKRPQDRKYGYYVLPVLFGREFVA
                     RFEPVFDKKKRVLNIKNWWWEEGVVVTEDMKEAIRKCTDDFAGFLGAEKIAT"
     gene            2293..>2588
                     /locus_tag="PLD15_11550"
     CDS             2293..>2588
                     /locus_tag="PLD15_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64592.1"
                     /translation="MAGFTFLYFTGIYTIMTFELVLLIMNYRFSKGPSIAVPGVLRDV
                     YTPELVEKSILYAKARGELQIFSTLVEFTFVTAGIFWLFPLIERIAVSLAGSFV"
BASE COUNT          623 a          692 c          592 g          681 t
ORIGIN      
        1 ggagaagggt atcacaaaaa gcgcatagac cgtgaccata gtcaccctgt cgaaattgtt
       61 ccagtaaacg agcgtggcca cgactatgaa ggctaaaaag ctcagcccga aatacttcca
      121 gcgtttcgaa agttccgtgg cggccaggat ggctacgcct ggaatgatct caatcgcggt
      181 cgttacactt aaaccgtgag agagaacagc gaaccctata aatatcagtc ccaccgtcct
      241 gcctataatc gagatagact gattccgcga aagcgaaagg ccggccgaaa aaatggcttt
      301 tgcggcgaag gggatgagaa tgaaagatat gaaacagtcg gtacctatct cgtatcccat
      361 gcccgtgtag aacatcgcaa ggagaaaaac tatagccagt gcgatattcg aagaaaggtt
      421 gtctacggct ttttctcttt catccagatc tctaatcagg cccgacattc tcaaaatcgg
      481 aaagaggaaa agtccggtca ggagaaagga aaaccatgga ctgaccgtct gaataagtgt
      541 cagtgcgacc gagatgagta tcacgtcgaa aatcaggatg aacgtctttt gaagcatatc
      601 aatcaccttc ttcgataatg aacaggtctt ccgctctgca gcgcaacaca cgcgcgatct
      661 tgagagccag cttgacagaa ggattgaacc gccccttctc aatcgcgatg atcgtctgcc
      721 tgcttactcc tacaagctct gccagagatt cctgagttat ctcgtcgttc ttgaacctga
      781 attcccgcag cctgttcttc atatccacca cctgtgaata ttgtacataa tactttacat
      841 tatgtcaagt attatgtaca taaggtcgga ataccaaatc acgatccggc atgacgggaa
      901 gggattgtca tcccgctgtg ctcttaaacg gatctcatct cttaagtctc ttcctctctc
      961 ctcttctcgc gctccttaac cgacgaagaa cctgaacgcg cagcgtcgga gcttctacta
     1021 tgtcgctatt ttctccgcgc cgaggaagcc ggcgaagtcg tcggtacatt tccgtatggc
     1081 ctctttcata tcctccgtga cgacaacccc ttcttcccac caccagttct tgatattcaa
     1141 taccctcttc tttttatcga aaacaggctc gaaacgcgcc acaaattccc ttccaaagag
     1201 aacggggaga acgtagtaac cgtacttcct gtcctgcggc cgcttgtaca cctcccaggt
     1261 gtattcgaac tggaagaggc tctttatgaa cttcctgtcc cacaacaggt tgtccagcgg
     1321 cgctataaaa gaagcttccg gagcgccagt gctttcatgg cttcgatcga gaagctctct
     1381 attaacgctc gaagtataaa ccgggtaatc gaacccttcg atttccagct cgatcaattc
     1441 gcccgagcgc accatttcct ctatcgcggc agtccgttta gcgctgttaa ggccctttat
     1501 tccgagccag gcgtcgccag atctcgacca gaaaaggccg atgctgtcga tccttctgct
     1561 tatatgccag cggtgataag cctcctcggt cgtgttggga tctagcctgt cgaaaatatc
     1621 ttgcggtatg tgtcttcctg ccagatcgta gtacttcctg gttccggccc tgtgatgtac
     1681 cacgagctct ccccaccagt acatactctc gagtgcggcc cgggaggctt tggcgggcgc
     1741 ccagtaccag cccacctttt tatcgaaatc gaagtcctgg gaggagagcg gacccctgac
     1801 ctctatatcc ttccttatct gttcgagtat ctcgtctatg tgttcgcttc tcctgatatg
     1861 gtctttcgca tcttccctga atctctgaaa ataaggccag tcttctatcg ggtagatgga
     1921 catattcttg tcccagccgt cgagcagctt tctatcctcg tatagcaacc tgtaaagcat
     1981 atcgggcctg taattcttca ctctcgactg gagaacgagg tcggtattta tcccggcgat
     2041 gttcagagga tcgaattgca aacagccaac cttcctgaca aaggcgagta tcccctcgcc
     2101 ccccgaaagc ctccttggag gcagcagatt ctggtaggag agcaaaaacc tcttcgcggc
     2161 ttcttttgag atcttcattc ccggcacaac tcctttcgtg tcatctcttc tttttgaatg
     2221 ttatcatatc gactccagga tcatagggcg cccgtgaagg ttttatcgta gaattcatta
     2281 cgaggtgagt ggatggcagg ctttactttt ctctatttta caggtatata cacaatcatg
     2341 accttcgagc tggtattact gatcatgaac taccgcttct cgaagggacc gtcgattgcg
     2401 gtacccggtg ttttgagaga tgtttatacc ccggaactcg ttgagaaatc gatcctctac
     2461 gctaaggcaa ggggagaatt acagatcttt tcaactcttg tggagttcac gtttgtcacg
     2521 gccggtattt tctggctctt tccgctgatc gagaggatcg cggtatcgct ggccggaagc
     2581 ttcgtatg
//
LOCUS       DAOOLY010000314         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_49372_length_2579_cov_2.557052, whole genome shotgun sequence.
ACCESSION   DAOOLY010000314 DAOOLY010000000
VERSION     DAOOLY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49372_length_2579_cov_2.557052"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2579
                     /locus_tag="PLD15_11555"
     CDS             <1..>2579
                     /locus_tag="PLD15_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPB64593.1"
                     /translation="TPVRIASGKMTSVFLSGLNIGKQYTLKVVSKDGRGAQAEKEILL
                     TPGNRAPSVVLSRPETEGLVEPTAVTFEWSGSDSDGDRLKYQLHINSPGQRQVFENIE
                     VERFTVRDLQEEREYRWYVIASDDKGAFARSSERVFTTGKKSLEVVTLLLPEQEALEL
                     PLRNAEFTWKIDQEPENAQYRFTLSRDPKMEEIVIQRTVKEKALKVNQEFQSNSRYYW
                     SVDLLIGEEVRTGEVRTFKTFNNPPEVPTARSPVNGATDVSTERLALVWDASDSDGVV
                     ASSIVHFKISGGEEKVLNTSSNSVVVDGLLSGREYRWWVEIIDDGGKKNKGPEWTFTT
                     GNQSPTVAFTLPSGTLLEGVAAPLQFTWELDDPEGESLSVSVFLSETGKESATPVASG
                     NNLLSYRVERIEEGRDYTLTVVATDPGGKEGRASIAFKGRYSPITYIYPTSGGLYSGE
                     RNFEWSHVGAGEGYIFRLYDEGFNPLVRARTTEANYRPEITFETGKQYYWAVSVIDGD
                     REYAGSPIGFVNGSGNSVTLLTPQNNAIADSSGVTLSWEVQGPQNNISRTELLFGEIG
                     NLSPIIIGPNSRSYTIGRLGGGKLYEWYVQLTDVSGNTSRSEIRRFEVPSEITNNPPL
                     IALTSPQEGAVVDQSSVELRWSASDEDGDRLSFSLYFGKNNDMLLVSESITSNSFTVT
                     NLEPGNSYGWYIRVSDGRITLDSPLRRFTTTQRAVTIDTPSPSNGSEGIGIKPVLSWR
                     TQGTTGGEFRIYLGTSRSFLPMVGTSASSSFAYTGELEMETTYFWRVELWEGGNLVSS
                     SPLWQFSTESRPVQVSDSTTVAVYVNGTLNVISMDNTSGTVSVIRNPYQYQGKVSPVT
                     NGD"
BASE COUNT          682 a          637 c          710 g          550 t
ORIGIN      
        1 aacacctgtg aggatcgcgt cgggaaagat gacctccgtc ttcctctcag gtcttaacat
       61 cggaaaacag tacacgctca aggtagtttc gaaagatgga cggggcgcac aggcagagaa
      121 ggagatactt cttactcccg gcaaccgtgc cccttccgta gtactttcca gacccgagac
      181 cgaaggtctg gttgagccta cggcagtgac cttcgaatgg tcgggtagcg acagcgacgg
      241 cgacagactc aagtaccaat tgcacatcaa ttcgcctgga cagagacagg tattcgaaaa
      301 catcgaagtt gaaaggttta ccgtcagaga tctccaggag gagagggagt atagatggta
      361 cgttatagcg agcgacgaca agggagcatt cgccagaagc tccgaaagag tctttacgac
      421 tggcaagaaa tccctcgaag tcgttaccct gctcttgccg gaacaggaag ctttggagtt
      481 gccactcaga aacgccgagt tcacctggaa gatagatcag gagccggaaa acgcccagta
      541 ccgattcaca ctttcgagag acccgaaaat ggaagagata gtgatccaga gaacagtgaa
      601 ggagaaggca ctgaaagtca atcaggagtt ccagagcaat tcgcggtatt actggagtgt
      661 cgatctactg atcggcgaag aagttagaac gggtgaagtt agaaccttca agactttcaa
      721 caacccgcca gaggttccga ccgcgaggtc gcctgtgaac ggtgccaccg acgtttcaac
      781 cgaaaggctc gctcttgtct gggatgccag cgatagtgac ggtgttgtag ccagttcgat
      841 cgttcacttt aaaataagtg gcggtgaaga gaaggttctg aacaccagct cgaactcggt
      901 ggtggtggat ggactgttat ctggccgcga atacagatgg tgggtagaaa tcatcgacga
      961 cggcggaaag aaaaacaaag ggccggagtg gacctttacg accggcaatc aatcccccac
     1021 cgtagccttc accttaccgt ctggcacact cctggaaggc gtagccgctc ctttacaatt
     1081 cacctgggag ctcgacgatc ccgagggaga aagtctctcc gtatctgtct tcttgagcga
     1141 aacaggcaaa gagagcgcaa cccccgtggc gagtggaaac aatctgctct cttacagagt
     1201 tgagcggatc gaggaaggta gggattatac cctgactgtt gtagccaccg accccggagg
     1261 caaagaggga agggcctcga tcgctttcaa aggcagatac tcgccgatca catatatcta
     1321 tcccacaagc ggtggccttt actctggcga gagaaacttc gaatggtcgc acgtaggcgc
     1381 cggtgaaggt tacatattta ggttgtacga cgaagggttc aatccactgg ttagggccag
     1441 aacgacggag gccaattaca gaccggaaat aacttttgaa acagggaagc aatactattg
     1501 ggccgtatcc gttatcgatg gagatcgtga atatgccggt tcgccaattg gattcgttaa
     1561 tggttcgggt aacagcgtaa cactgctgac tccacagaac aacgcgatag cagattcttc
     1621 aggagtcacg ctaagctggg aggttcaggg cccacagaac aacatctcgc gtaccgagct
     1681 cttattcgga gagatcggca atctctctcc cataataatc ggtcctaact ctaggtccta
     1741 taccatcgga agactcggtg gaggaaagtt gtacgagtgg tacgtacagc tcacagacgt
     1801 gagtggaaat acttccagaa gcgagataag aaggttcgaa gttccgtcag aaattacaaa
     1861 caacccgccg cttatagctc tcacgtcacc gcaagagggt gcagtagtcg atcagagttc
     1921 cgtcgaattg agatggtcgg ccagcgacga agatggcgac cgtttaagtt tctctctgta
     1981 ctttgggaaa aacaacgata tgctccttgt aagcgagagt ataacctcta attccttcac
     2041 ggtgaccaac cttgagccgg gcaacagcta tggctggtat atcagagtga gcgacggcag
     2101 gataacgctc gattctcctc tgaggcgttt cacaacgacc cagagagccg tgactatcga
     2161 cactccgtct ccatcgaacg ggagtgaagg gataggcatt aaaccggtcc tctcctggcg
     2221 aacgcaggga acgaccggtg gtgagttcag gatctatctc ggtacttcga gatcgttcct
     2281 gccgatggtc ggaacctcgg cctccagctc cttcgcttac acgggcgagc tggagatgga
     2341 aaccacttac ttctggaggg ttgaactttg ggagggtgga aatctggtct cctcatcgcc
     2401 gctctggcag ttctccaccg agagcaggcc tgttcaggtt tccgattcca ctacggtggc
     2461 tgtgtacgtt aacggtacgt tgaatgttat ctctatggat aatacatccg gtacagttag
     2521 cgtgatcagg aacccctacc agtaccaggg aaaggtttcc ccggtcacca acggcgatc
//
LOCUS       DAOOLY010000315         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_50177_length_2550_cov_3.649699, whole genome shotgun sequence.
ACCESSION   DAOOLY010000315 DAOOLY010000000
VERSION     DAOOLY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50177_length_2550_cov_3.649699"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLD15_11560"
     CDS             complement(<1..240)
                     /locus_tag="PLD15_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB64594.1"
                     /translation="MQLNPLIYNLGRCNLFAGIPVGQLEEIFSKVKHRICKFLPGELI
                     QEQGSSCEEMLVLLLGEVKAQMQDFSGKVVQIERIK"
     gene            548..1120
                     /locus_tag="PLD15_11565"
     CDS             548..1120
                     /locus_tag="PLD15_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPB64595.1"
                     /translation="MRLFIFDVGGVVAENTSVVPRICDYLDIPQDRFIELAGHESIQA
                     LQMGKMTEEAFAIHLSEKTGKEVPANIWSIFFNPVPIEGTVKTIEKLKTRYRVIAGTN
                     TIDPHYRIHKSRNDYGLFDEVYASHLMGLAKPDPEFYRHILCAENCPPEEAFFVDDTL
                     KNVLAARNLGIISFHFTEPSALEERLKEFL"
     gene            1146..2489
                     /locus_tag="PLD15_11570"
     CDS             1146..2489
                     /locus_tag="PLD15_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB64596.1"
                     /translation="MKRLTVILFVLLMLVIPIGAMAKTTITWWINPWRITPPGFPADK
                     AATEEDFPKWAAEEFMRLNPDVEIKYVVVGNTEYAQKMAAAIATGTQPDIFKGPVWDN
                     RWVGAGLLEPIDDYLSQEDIEDFYELALESGYVDGKHYIWPWNLGTNGMGTSMLLYTP
                     DFEKAGIDWKKIVEEGWTMEEFVEIAKKLTWDSDGDGKTDHYAISFGAQDTHNLMSFI
                     YAFGAELIKEDETKVVFNSQEAIDGLKFLVDLVEVHGVVPKGVEAMGVYDVIGNFHSH
                     RTSIGFGGPYEIGRITRYVLSGQLAEQFYPVIAPFPHVEGKKGASYASASGFVVFKQK
                     DPVKKEKVMEFAKFLTNRENTALLESLLYLTARKSVNAQLFKSDEYLNDQTQTFARIM
                     DETGMEFFGSQSFPWSEISKFFTSAMQGAFGLTKTPKEALDGFVTEANRALAYYY"
BASE COUNT          691 a          595 c          633 g          631 t
ORIGIN      
        1 cttgattctc tcaatctgaa cgaccttgcc cgagaagtcc tgcatctgtg ccttaacttc
       61 acccagaagg agcaccagca tttcctcgca gctacttccc tgttcctgta tgagttcccc
      121 tggcaggaat ttgcagattc tatgttttac cttagaaaat atctcttcca gctgacccac
      181 aggaataccg gcaaagaggt tgcaccttcc caggttgtat atcagaggat tcaactgcat
      241 tcaccacctt aatcagtttc ttcgtcgaca tcgatatgga gagccctttt tttcagttac
      301 aacaaatgag atgccttcca gactctcctg agtagattat acttatcgat tgctgaatat
      361 caacgattac tggccaaaaa tccctttcga tttctcttga ttttagatat ggccaaaagg
      421 tatcatatta aagtacactt tgatatccta atttcgcggg ttgtttcgga tgggattttc
      481 gggtttaatt tccctgtccc gtcgtagtta atgtactttt gtaaatacat tatttgtgga
      541 ggctgatttg agacttttca tcttcgatgt tggcggcgtt gttgccgaaa ataccagcgt
      601 agttccccgc atatgcgatt atctggacat tcctcaggat aggttcatcg aattggcagg
      661 ccatgaaagc atccaggccc ttcaaatggg aaagatgacc gaggaggctt ttgccataca
      721 cctctctgaa aagacaggca aagaagtacc ggccaatatc tggtcgatct tcttcaatcc
      781 cgtgccaatt gaaggcacgg taaagacgat cgagaagctg aagaccaggt acagagtaat
      841 agccggaaca aacaccatag atccgcacta caggattcat aaaagccgaa acgattacgg
      901 cttgtttgac gaagtgtacg cctcgcactt gatgggactg gccaaacccg atccggagtt
      961 ttaccgtcat atcctttgtg ccgaaaactg cccgccggag gaggcatttt tcgtcgacga
     1021 tacattgaaa aatgttctgg cagcgagaaa tctgggtatt atatcatttc attttacaga
     1081 accttccgct ctggaagaaa ggctcaaaga gttcctttga gtaaaaatcg aaaggggtgt
     1141 agagaatgaa aagactcact gtaattctat ttgttttgtt gatgctggtt attcctatcg
     1201 gcgcgatggc gaaaaccacc ataacgtggt ggatcaatcc ctggagaata acgcccccag
     1261 gattccccgc cgacaaggca gcaaccgaag aggacttccc gaagtgggcc gcggaggagt
     1321 ttatgaggct caatccggat gtggagatca agtatgtcgt tgtaggcaat accgagtacg
     1381 cgcagaagat ggccgccgca atagcgacgg gaacgcagcc ggatatcttc aaaggacccg
     1441 tctgggataa caggtgggta ggggcaggcc ttctcgagcc gattgacgac tatctcagcc
     1501 aggaagacat cgaagatttc tacgagctag cactggaaag cggatatgtt gacgggaagc
     1561 actacatctg gccgtggaat ctcggcacaa atggaatggg cacatcgatg cttctgtaca
     1621 ctcccgattt tgagaaggcg ggaatcgact ggaagaagat agtcgaggaa ggctggacta
     1681 tggaggagtt cgtcgagatc gccaagaagc tcacatggga ttccgacggt gacggcaaga
     1741 ccgaccatta cgcgataagc ttcggtgccc aggataccca caatcttatg agcttcatct
     1801 acgccttcgg cgcggaactt ataaaagaag acgagacgaa agtcgtattt aactcccagg
     1861 aagcgatcga cggcctgaag ttcctggtgg atctggttga agttcatggc gtggttccaa
     1921 agggcgttga agcgatgggt gtttacgacg tgataggtaa cttccactct cacagaacca
     1981 gtatcggttt cggaggtcca tacgaaatcg gaaggatcac cagatatgta ttatccggcc
     2041 agcttgccga acagttctat ccggtcattg ctcccttccc tcacgtagaa ggaaagaaag
     2101 gggcatctta cgcttctgcg agtggcttcg tggtcttcaa acaaaaagac ccggtgaaga
     2161 aggaaaaagt aatggaattt gccaagttcc tcaccaacag agagaacacg gcgcttctcg
     2221 agagcctttt gtatctgacg gcaagaaagt cggtaaatgc ccaactattc aagagcgatg
     2281 aatacctcaa cgatcaaacg cagacctttg cgagaataat ggatgaaacc ggcatggagt
     2341 tctttggatc gcagagtttt ccgtggtcgg agatatccaa attcttcaca agtgctatgc
     2401 agggtgcatt tggattgacc aaaaccccga aggaagccct cgacggtttc gttaccgaag
     2461 cgaacagggc tctggcttac tactattaat ccaaagcaat cagtcaaagg aacagcctcc
     2521 gcccccgtcg ggtctggggc tgttcctgtt
//
LOCUS       DAOOLY010000316         2532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_50666_length_2532_cov_5.508680, whole genome shotgun sequence.
ACCESSION   DAOOLY010000316 DAOOLY010000000
VERSION     DAOOLY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50666_length_2532_cov_5.508680"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLD15_11575"
     CDS             <1..670
                     /locus_tag="PLD15_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPB64597.1"
                     /translation="VKQNLYERSAQAAGKVMVVGAGGLGAPLIKILLDRGLKDIAIVE
                     PGEIKLNNLHRQILYDYGDVGYPKATILERKLSRMYPSARVKIYRERFTEALLRSEKP
                     DLVVSCVDNYGARYEINDSSYRSGIPYVDSAVDGFSGYVMFRRRGLACYRCFMGDDRK
                     DTTAPKGILPFTSYFGAMLQAAVTIDFLNRRGSIEKVFWFDLRNDMYEEIDIGKRENC
                     KVCN"
     gene            680..874
                     /locus_tag="PLD15_11580"
     CDS             680..874
                     /locus_tag="PLD15_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64598.1"
                     /translation="MAMEVVYGQKRWSFDRAMPLERLVEETGLRREAVLVMADGRLIR
                     EGETIPAGSKVLIISAANGG"
     gene            1021..2367
                     /locus_tag="PLD15_11585"
     CDS             1021..2367
                     /locus_tag="PLD15_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPB64599.1"
                     /translation="MEKLEKKRVLILGAAGRDFHNFNTYYRDNEEFEVVAFTATQIPG
                     IDDKKYPAELAGRLYPNGIPILPEDNFGKLIDQYKIDQVVLAYSDLPHQYVMERAAIA
                     NAHGADFVLLGPSKTMIKSSKPVISIVAVRTGCGKSQTTRRVLDILRAKGKKVVSIRH
                     PMPYGDLVKQKVQRFADYADLDRHECTIEEREEYEPHIDRKAVIYAGVDYEAIMREAE
                     KEDVDVIIWDGGNNDFSFYKTDLYITVVDPHRPGHETTFYPGFTNLMMADVVVINKEE
                     TASPEGIETVRKNIAKYNPDAIVVDGASPITIEGGKASEIKGKRVLVVEDGPTLTHGG
                     MRYGAGWVAAKKFGAKEIVDPRPYAVGSIVATYEKYNHIDEILPAMGYGEKQMKELEE
                     TINKADVDLVIIGTPIDLRRVIDINKPAVRIGYELQEIGKPDLEEIIDEFLKKHKI"
BASE COUNT          702 a          626 c          717 g          487 t
ORIGIN      
        1 ggtcaagcag aatctttatg agcggagcgc ccaggccgcc ggcaaagtaa tggtggtcgg
       61 tgccggcggc ctgggcgctc cgctcataaa gattctgctt gaccgcggcc tgaaagatat
      121 cgcgatcgta gagcccggcg agataaaact taacaacctg caccgacaaa tattgtacga
      181 ttatggtgac gttggatatc ccaaagcgac gatactcgag agaaaactct ccagaatgta
      241 cccttcggcg agagtgaaga tctacaggga gcgtttcacc gaagctcttc tcagaagcga
      301 gaaaccggac ctggtggtga gctgcgtgga caattacggc gcacgttacg agataaacga
      361 ttcctcttac cgctcgggga ttccatacgt tgactcggcc gtagatgggt tcagcggcta
      421 cgtgatgttc aggagacgcg gcctggcctg ttacagatgc ttcatgggcg acgacaggaa
      481 ggataccacc gcgccgaagg gcatactgcc cttcacgagt tacttcgggg ctatgcttca
      541 agcggccgtg acgatcgact tcctgaaccg gagagggtct atcgagaaag tcttctggtt
      601 cgatctaagg aacgatatgt acgaagagat cgatatcgga aaacgagaaa actgcaaggt
      661 ctgcaattga agaggtgaaa tggcgatgga agtggtttac ggacagaaaa gatggagctt
      721 cgacagggcg atgcccctgg aaaggctggt cgaggaaacg ggcctcagga gagaggcggt
      781 cctcgtaatg gccgatggcc ggctgataag agagggcgaa accattccgg ccggatctaa
      841 ggtgcttata ataagcgcgg caaacggagg atgatacgct agcataccca cgcataccgt
      901 cgtatagtgc catatatagg ggttttttgg cccctattgt ttttgttggg cggttggtcc
      961 tttttgaaaa ctgtctttat gtactgctag aatcttatag ataacggaag aaatacggag
     1021 gtggagaaat tggaaaagaa acgtgttctg atacttggag ctgccggaag agacttccac
     1081 aacttcaaca catattaccg agacaacgaa gagttcgaag tagtagcctt cacggcgacc
     1141 cagattccgg gaatcgatga caagaaatac cccgccgagc tggccggcag gctttacccg
     1201 aacggcatcc ctatactgcc cgaagacaac tttggaaaac tgatcgacca gtacaagata
     1261 gatcaggtag tccttgccta cagcgatctc ccgcaccagt acgttatgga aagggccgcc
     1321 atagccaacg ctcacggggc cgatttcgtc ctgctcggac cttccaagac gatgataaaa
     1381 tcatcgaaac ctgtgatctc gatcgtcgcc gtcaggacgg gctgtggaaa gagccagacc
     1441 acaagaaggg tgctcgatat cctcagggcc aagggaaaga aagtagtgtc tataagacac
     1501 ccgatgcctt atggcgacct cgtgaagcag aaggtccaga gattcgccga ctacgccgat
     1561 ctggacaggc acgaatgcac gatagaggag agagaagagt acgagccgca catcgacagg
     1621 aaggccgtta tatacgccgg agtcgattac gaagcgatca tgagagaggc cgaaaaggaa
     1681 gacgtggatg ttatcatctg ggacggcgga aacaacgact tctccttcta caaaaccgat
     1741 ctctacataa ctgtcgtaga tccgcacagg cccggtcacg agaccacttt ctacccgggc
     1801 ttcaccaacc tcatgatggc cgatgttgta gttataaaca aagaagagac agcctccccc
     1861 gagggaatcg aaacggtccg caagaacatc gccaaataca accccgacgc catagtcgtc
     1921 gacggcgcct ccccgataac gatcgaaggc ggcaaggcat cggagataaa gggcaagaga
     1981 gtgctcgtgg tcgaagacgg tccgacgctg acgcacggtg gaatgagata cggagccggc
     2041 tgggtggccg ccaagaagtt cggcgctaaa gagatcgtcg atcccagacc gtacgccgtc
     2101 ggctcgatcg tggccacgta cgagaagtac aaccatatcg acgagatcct gccggccatg
     2161 ggttacggcg agaaacagat gaaagaactg gaagagacca taaacaaggc cgacgtagat
     2221 ctggtcatca tcggcacgcc gatagatctc agaagagtca tagatataaa caagccggcc
     2281 gtgaggatag gctacgaact tcaggaaata ggcaagccgg atctggaaga gatcatagac
     2341 gaattcctca agaaacacaa aatctgaacg gatttaaccg aagggaccgg gtgaaaccgg
     2401 tccctttttt tctcaacgcc gtcccgccgg ctcctcgtta tatctagagt tgaacgacct
     2461 tcgtgagatt ctgcggtcta tcgggatcga gactcttagc gatcgcccgt tcgacgccga
     2521 gcaactggag ag
//
LOCUS       DAOOLY010000317         2516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_51135_length_2516_cov_3.780171, whole genome shotgun sequence.
ACCESSION   DAOOLY010000317 DAOOLY010000000
VERSION     DAOOLY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51135_length_2516_cov_3.780171"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLD15_11590"
     CDS             <1..774
                     /locus_tag="PLD15_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPB64600.1"
                     /translation="IDQSPIGRTPRSNPATYTGLFDFVRDLFSKTQEARARGYSKGRF
                     SFNVKGGRCEACQGHGMIKIEMQFLPDVYVECDVCKGRRYNKETLEIKYRGRSISDVL
                     NMTVEEASTFFERIPVLNRILELLNDVGLGYIRLGQPATTLSGGEAQRIKLASELKKK
                     STGSTFYILDEPTTGLHFDDVSKLVKVLKRLVELGNTVVVVEHNMDVIKNADYIIDLG
                     PEGGARGGEVVISGTPEEVAETRFSHTGYYLRKLLNPVR"
     gene            complement(789..2096)
                     /locus_tag="PLD15_11595"
     CDS             complement(789..2096)
                     /locus_tag="PLD15_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB64601.1"
                     /translation="MPKVPIEENYLESDEFRFDGKAALILSGGGARGGYQIGCWKALK
                     DHGIKIAGVYGTSVGSINAAAVAMDNFELATELWKNMDYSTVMEITPEVESVIQMAKA
                     KAGIREVFMGIKLLMDGRGIDITPLRQCLHSRVDEEYVRKSGIDFGLVTYSLSDMRPV
                     MLYINEIPRGMLVDYILASANYPVFKREEFDRRKFIDGGVYINIPIDMARKKGFTSMV
                     VIDIGTKSLIDRLRVIAGTFDRDKTLYIRPKRHFGSPMQFEREITMKYLIEGYLDTLT
                     ALGVVQGSYTYIYETEDILAKLFWSIDEETFSRALGILNIERKKTESPMYFYFRQLLP
                     LLEVGFKTYGSKRILCDLADYTFELMEIEELELYSGFDFFEAISAKPPVSFENVRGAT
                     INDIPLAGLLEMLSLVYRKSRFRVKRPARFDLYRSSLDFLGAK"
BASE COUNT          598 a          642 c          619 g          657 t
ORIGIN      
        1 atagatcaga gccccatagg cagaactccc agatccaacc cggccaccta taccggtctc
       61 ttcgacttcg ttagagacct cttctccaaa actcaggaag ccagggcgag aggctactcg
      121 aaggggcggt tctccttcaa cgttaagggc ggaaggtgcg aagcctgcca ggggcacggg
      181 atgataaaga tcgagatgca gttcctgcct gacgtttacg tggagtgcga tgtctgcaaa
      241 ggtcggcgtt acaacaaaga aaccctggag ataaagtaca ggggcagatc gatctcggac
      301 gttctgaaca tgaccgtgga ggaagcatct acgtttttcg aaaggatacc ggttctcaac
      361 aggatactgg agctgctcaa cgacgtcggg ctgggttata taaggctggg ccagccggcg
      421 accacacttt ccgggggaga ggcacagaga ataaagctgg cttcggaatt gaagaaaaaa
      481 tccaccggca gcaccttcta tattctcgac gagcccacca ccggacttca tttcgacgat
      541 gtgtccaaac tggtaaaagt tctcaaaaga ctggtcgaac tgggaaacac cgtggtcgtg
      601 gtggaacaca acatggacgt gataaagaac gccgactata taatcgacct cggaccggag
      661 ggaggggcca gaggcggaga ggtcgtgatc tcggggactc cggaggaggt ggccgagacg
      721 aggttcagcc acaccggcta ttatctgaga aagctattga accccgtccg gtaatcacgt
      781 ggagattttc acttcgctcc caggaagtcc aggctactcc ggtaaagatc gaatctggcc
      841 ggtcttttca ccctgaatct gctctttcta tatactagcg ataacatctc cagcaggccg
      901 gccaggggta tatcgttgat agtggcccca cgaacgtttt cgaaagagac cgggggcttt
      961 gcggatatcg cttcgaaaaa gtcgaacccc gaatagagct ctaattcttc gatttccatc
     1021 agctcgaagg tgtaatcggc aaggtcgcag aggatgcgtt tgcttccgta ggtcttgaag
     1081 ccgacttcca gaagcggcag aagctgtctg aaataaaagt acatagggct ttccgttttc
     1141 ttgcgttcta tgttcagaat accgagcgcc ctggaaaacg tttcctcgtc tatagaccag
     1201 aacagttttg ccagaatatc ttccgtctcg tagatgtacg tgtaactgcc ctgaacgaca
     1261 cccagagccg ttagggtgtc taggtatcct tcgattagat atttcatcgt tatctcccgt
     1321 tcgaactgca tggggctgcc gaagtgtctt ttcggcctta tatacagagt tttgtctctg
     1381 tcgaaggttc cggctatcac tctaagtctg tcaataaggc tcttggtgcc tatgtcgatt
     1441 actaccatgc ttgtaaaacc cttttttctg gccatgtcta tgggaatatt tatataaacc
     1501 ccaccgtcta tgaacttgcg gcggtcgaac tcctctcttt tgaaaacggg gtaattggcg
     1561 ctcgcaagaa tgtaatcgac gagcattccc ctgggtatct cgtttatgta cagcatcacc
     1621 ggcctcatat cgcttagaga atacgtgacc agcccaaaat ctatacccga tttcctgacg
     1681 tactcttcgt cgactctgga atgaaggcac tgcctcaggg gagttatatc gattcccctg
     1741 ccgtccatca gaagcttaat gcccatgaag acctccctga tgccggcttt ggctttagcc
     1801 atttgaatca ccgactccac ttcgggcgtt atctccatca ccgttgaata atccatgttt
     1861 ttccacagtt ccgtcgccaa ctcgaagtta tccatcgcga ccgccgcggc gtttatcgag
     1921 cctaccgagg ttccgtaaac accggctatc tttataccgt ggtccttgag ggctttccag
     1981 cagcctatct ggtagccgcc cctcgcaccg ccgccggaga gtatcagggc cgcctttcca
     2041 tcgaaacgaa actcatcgct ctcaagataa ttctcctcta tcggtacttt tggcatgccg
     2101 gtcacctcac ttcgaaaaat attgaaactc ccttcattaa aagaatgctt cgctctctcg
     2161 ctgggaataa taacacgaaa tagcgattaa aaagcctata tttctcgcag gttgatttcg
     2221 cgtcgatgaa tatgctaaac tatttccagc gtgacagaga tgatgagaaa gttgcgcctt
     2281 tatggcgtcg gctttctttt ttttgatcta gtttttgatc tcgtgcctgg attttgctgt
     2341 cataatcgct gtaacttatt ttcggtactg tcggataagc ggggtgatac acttgcggcg
     2401 tcgttccagg gaggttataa ctggctggtt aacggttacg cctgcgctcg ttgttcaggt
     2461 gattttatct atcttccact cgcctgggct ttctacgtct cgtttcatag ctggaa
//
LOCUS       DAOOLY010000318         2505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_51456_length_2505_cov_2.656735, whole genome shotgun sequence.
ACCESSION   DAOOLY010000318 DAOOLY010000000
VERSION     DAOOLY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51456_length_2505_cov_2.656735"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLD15_11600"
     CDS             complement(<1..367)
                     /locus_tag="PLD15_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64602.1"
                     /translation="MNDRKKFSSVPLLLIVATILSYIFTSMTVWSTDGKYLFLSRYLR
                     INQHERVISGENFAPDQYRFGSYYLVEYFFKYIPIPWLDENTSEVSKMLLSTEYWTEE
                     ARGMVEAYFPLQERERIVSD"
     gene            complement(364..1677)
                     /locus_tag="PLD15_11605"
     CDS             complement(364..1677)
                     /locus_tag="PLD15_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64603.1"
                     /translation="MGFEGAVNDRSKKKVFLIALFFSVVLSYIFTSMILWTTESRFLT
                     VSRYSKVMRHREAIEGRSFAPDQYRFGSYYLVEYIFKHIPLRWYDVFNDIVAEGLDPT
                     GWGEGTEKSVELFFPEEDRAVIIKEIESTIDRIIDSFSPNNLLLRNMLKAAIDSFGWQ
                     EYINDIEKTTMLIGKNIPGEFKALIEKDSAESALVNGHVTFRFFFTATTLLLVFLLCA
                     QFTDALTSLLGMSLFAALLPMVAQDFMQAETMLSATVFAGFLVALLKNKSYLLLLLLV
                     LLGCTARTDQTLFAGVIYLLYKVPGAIKRRKWLSLLAGLSLVFIPLVATLLLSEVIYA
                     GSEYYLDFIQLEYNLNHPWAWIYPLIFLAIPLAFSPMIRNIDFFRKTWLWVPPFMVMN
                     YLFARPAEVRLLLPVLIYSVPYVVAGVKEVLCHFDYDRDRKGGGS"
     gene            complement(1717..>2505)
                     /locus_tag="PLD15_11610"
     CDS             complement(1717..>2505)
                     /locus_tag="PLD15_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB64604.1"
                     /translation="IYNKYGIPVLYNFEESDMRWIEYTPKSKLNVLPRIFPEGIRLPD
                     YFFGKNIIQLPTVKCHIYTTTTGAMKNAFGGLLNVKRHYTHSWIHETLVDLLKIQKEI
                     HTGLFAFMDGTTAGNGPGPRTLIPVRTDYILASQDQVAIDAVAAKMMGFNPMDLKYIR
                     LADEEGLGNGKPENIELVGEDISKVNFKFHVGDNFASKVGDLLWFSPLKIFQKLFFHT
                     PLVKVFVAASDSYHDRYWWKKHGTKIFQQWKKESPWGRLWESYR"
BASE COUNT          627 a          604 c          616 g          658 t
ORIGIN      
        1 ggtcggatac aattctttct cgctcttgca agggaaaata ggcttccacc attcctcttg
       61 cttcttcggt ccagtattcc gtggatagga gcatcttcga tacttcagac gtgttttcat
      121 cgagccaggg gataggaatg tatttaaaga aatactccac aagatagtaa ctcccgaaac
      181 ggtattgatc gggagcaaag ttctctccgg agattaccct ttcatgctgg ttgattctga
      241 gatatctgga gaggaagaga tacttcccgt cggtactcca gacggtcatg gaggtgaaga
      301 tgtaagaaag tatagtcgca acgatcagaa gcagcggcac gctggagaat ttttttcggt
      361 cgttcatgag ccgcctcctt ttctgtctct gtcgtaatcg aaatgacaga gtacttcttt
      421 tacacctgcg acaacgtatg gaacgctgta tatcaatacc ggcaggagga gtctgacctc
      481 ggccggtctt gcaaacaggt aattcataac catgaatggc ggaacccaga gccaggtttt
      541 tctgaagaaa tctatgttcc tgatcatcgg actgaaggcc aaaggaatgg ccagaaatat
      601 cagggggtag atccaagccc aggggtggtt caggttatat tcgagctgaa tgaaatcgag
      661 gtaatactcc gagccggcat agattacctc gctcagtaat aacgtagcga ccagagggat
      721 aaataccagc gaaagcccgg ccaataggga gagccacttt cttctcttta tcgccccggg
      781 tactttgtag aggaggtata tcacaccggc gaaaagcgtt tggtctgtcc tagccgtgca
      841 gcctagcaga acgagcaaaa ggaggagtag atagcttttg tttttcagca aagccacaag
      901 aaagccggcg aagacggttg ccgatagcat ggtttcggct tgcatgaagt cctgggctac
      961 cataggaaga agggcggcaa aaagcgacat acccaaaagc gacgtcaacg cgtcggtaaa
     1021 ctgcgcgcac aagagaaaaa cgagcaggag agtagttgcg gtgaagaaga atctaaaggt
     1081 aacgtgtcca tttaccagtg cgctctcggc gctgtctttt tctatcaggg ctttgaattc
     1141 tccggggatg ttctttccaa tgagcatggt cgtcttctct atatcgttga tgtactcttg
     1201 ccagccgaag gagtcgattg cggctttcaa catgtttctc aaaagcagat tgtttggcga
     1261 aaacgaatct atgatcctgt ctatagtgga ttctatctcc ttgatgatta cggcacggtc
     1321 ctcttcggga aagaagagct cgacgctctt ctctgtgcct tcgccccagc ctgtcggatc
     1381 gagtccctcc gcgacgatgt cgttgaagac atcgtaccat cttaatggta tgtgtttgaa
     1441 aatatattcg accaggtaat aactgccgaa gcgatactga tcgggcgcaa agctccttcc
     1501 ttctatggct tctctgtggc gcatcacttt actgtaacga gaaaccgtca ggaacctact
     1561 ttcggtcgtc cagagtatca tcgaagtgaa gatgtacgaa agaacgaccg aaaagaaaag
     1621 cgcaatcaga aagacctttt tcttcgagcg atcgttcacc gccccttcaa aacccatgca
     1681 tccttccctc cgctccgaga aattatcaaa tccggtttac ctgtaagatt cccaaagtct
     1741 tccccatgga ctctctttct tccactgctg gaagatcttt gttccatgtt tcttccacca
     1801 gtacctgtcg tgatacgagt ccgaagccgc aacgaagacc ttaaccagcg gggtgtggaa
     1861 gaagagtttc tggaatatct tcaacggact gaaccatagc agatcaccga ctttgctggc
     1921 gaagttgtcg ccgacatgga acttgaaatt gactttagag atgtcttcgc cgaccagctc
     1981 gatgttctcg ggttttccgt ttcccagacc ctcctcgtcg gccagcctta tgtatttcag
     2041 gtccatcgga ttgaagccca tcatcttcgc ggcgaccgcg tctattgcca cctggtcctg
     2101 gctggccagt atataatccg tcctgaccgg tatcaacgtc cttgggcctg gaccgtttcc
     2161 cgcggtagtt ccgtccataa aggcgaagag cccggtgtgt atctctttct gtattttcaa
     2221 cagatcgacg agcgtctcgt gtatccagga atgcgtgtaa tgacgtttca cgtttaaaag
     2281 gccaccaaag gcgttcttca tagcccccgt cgtggtcgtg taaatgtggc acttcaccgt
     2341 ggggagctga ataatgttct taccgaagaa gtaatcgggt agtctaattc cttcagggaa
     2401 aattctggga agcacgttga gtttgctttt aggagtgtac tcgatccagc gcatatcgct
     2461 ctcttcaaag ttgtacagta cgggtattcc atacttgttg tatat
//
LOCUS       DAOOLY010000319         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_51722_length_2495_cov_5.045902, whole genome shotgun sequence.
ACCESSION   DAOOLY010000319 DAOOLY010000000
VERSION     DAOOLY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51722_length_2495_cov_5.045902"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /gene="dnaK"
                     /locus_tag="PLD15_11615"
     CDS             complement(<1..1479)
                     /gene="dnaK"
                     /locus_tag="PLD15_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPB64605.1"
                     /translation="MANKEYTVGIDLGTTNSVIAWVKQDGGVEVIPNAEGNRTTPSIV
                     SFSKTGEIVVGEPAKRQAILNADRTVRSIKRKMGSDYTIKIDEKDYTPQEISAYILKK
                     MKADAEAYLGGKVTKAVITCPAYFNDAQRQATKEGGIIAGMEVLRIINEPTAAAVAYG
                     IDKKQGDRKIIVYDLGGGTFDVSLLDIGDGVVEVLSTSGNNHLGGDDFDQLLIDYIAE
                     DFRKKNNVDLRKDKQAFQRLKDAAERAKIELSAKYETEISLPFITATADGPLHLEMKI
                     TRSTFESLIKDLVEGTREQIERAMSDAKVSPKEVDEILLVGGSTRIPMVQSFIKSIFG
                     KEPNKNINPDEAVAVGAALQSGILAGSVESDLVLVDVTPLTLGVEVMGGLLEPIIERN
                     STVPVKRSKVFTTAADGQTEVEVAVYQGERSMARDNVALGSFKLTGIAPAPRGIPQIE
                     VTFDIDSDGIVHVSAKDLGTNREQSMVVSGRQKMSEEEIKRIV"
     gene            1826..>2495
                     /locus_tag="PLD15_11620"
     CDS             1826..>2495
                     /locus_tag="PLD15_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPB64606.1"
                     /translation="MNDTVSIIIDWAVRIGVSLLIFIIARWLSKIFYNGFMKLSKKTG
                     IVNVQYQRTLKTLFNIAFFALAIFIIVSVLFKNLTPVLTGLGVSSIIIGLAIKEPLEN
                     FICGILIMLNKLIIEGEAVEISGQSGSIVEIKLNHVLLKTWDGKMITMPSRSVWTATI
                     VHFWPENIRRNDLNVGVSYSSDLNKVMKVLEESVNSYEKLYKDESHKPVIQFTGYGSS
                     SIDFV"
BASE COUNT          558 a          637 c          578 g          722 t
ORIGIN      
        1 gacgattctc ttgatttctt cttcggacat cttctgtctt ccactgacga ccatcgactg
       61 ctctctgttg gttccaagat ccttcgccga cacatgaact attccgtcgc tgtctatatc
      121 gaacgttacc tctatctggg ggattcccct cggggccggc gctataccgg tgagcttgaa
      181 acttcccagc gccacattgt ctctggccat agatctttcg ccctggtaaa cggcaacttc
      241 cacttctgtc tgtccgtctg ccgctgtcgt gaagaccttg gaccttttga ccggcaccgt
      301 agagttcctc tcgatgatcg gttccagcag gccacccatt acttcaactc ccagagtgag
      361 gggggttaca tccaccagaa cgagatcgct ttccacactt ccggcgagga taccgctctg
      421 gagagccgcc ccgaccgcaa cggcttcgtc cgggttgatg ttcttgttgg gttctttgcc
      481 gaatatcgac ttgatgaaac tctggaccat gggaattcta gtggagccac cgaccaggag
      541 tatctcgtcc acttcttttg gagagacttt ggcatcgctc atggccctct ctatctgttc
      601 tctggttccc tcgaccaggt ctttgatcaa agattcgaag gtcgatctgg tgatcttcat
      661 ctccaggtgg agcggtccgt cggccgtcgc ggttatgaag ggtagagaga tctccgtttc
      721 gtacttcgcc gagagctcga tcttggccct ttctgcggca tccttcagcc tctggaaggc
      781 ctgcttgtct tttctgagat cgacgttgtt tttcttcctg aagtcctcgg cgatgtaatc
      841 gatgagcaac tggtcgaaat cgtctcctcc gaggtggttg tttcccgagg tcgaaagaac
      901 ttcaactact ccgtctccta tgtctagcag cgacacatcg aaggttccgc ctcccaggtc
      961 gtaaacgatg atctttctgt ctccctgctt cttatcgata ccgtaggcaa ccgccgcggc
     1021 cgtcggttcg ttgattattc tcagaacttc cattccggcg attataccgc cttccttggt
     1081 ggcctgtctc tgcgcgtcgt tgaagtatgc cggacaagtg ataacggcct tcgtaacttt
     1141 tccgccgaga taagcttcgg cgtcggcctt catttttttg agtatgtacg cgctgatctc
     1201 ttgcggagtg tagtctttct catctatctt tatcgtgtaa tccgaaccca ttttcctttt
     1261 gatcgatctg accgttctat cggcgttcaa tatagcctgt ctcttcgccg gctctccgac
     1321 gactatctct ccagtcttgc tgaaggagac tatcgaaggt gtggtcctgt ttccttcggc
     1381 gttgggtatc acctcaacgc cgccatcctg ctttacccat gctatgaccg aattggtcgt
     1441 gcctagatcg attcctactg tgtattcttt atttgccatc ttgttcccct ccttgcgcag
     1501 ctcaacgctt ctacatttat attataggta agagtttgtg cactgtcaac aagaatagtt
     1561 ataaattata tactatcaaa atgcctatga taaaagagat atatagtgat aaataatatc
     1621 ataatcataa tgtatagcgt tcttagctat agactgacaa gaagaagatc cgacttttga
     1681 tatcctcgtt tttcaccgca aaatagggat agagattgat tcgcaagagt cagcccgtgc
     1741 taccaccatc tattccaggc ttttatcccg gtcgaaatat gtaagaatag agtatagttt
     1801 ctcaatcagg aaggagtgag tcattttgaa cgatacggta tcaataatca tcgactgggc
     1861 tgtcaggata ggggtaagct tactcatctt cataatagcc cggtggttat ctaagatctt
     1921 ctacaacggg ttcatgaaac tgtccaagaa aacgggtata gtgaacgtcc agtaccagag
     1981 gactctgaag acattgttca acatcgcttt cttcgcactg gccattttca taatcgtatc
     2041 cgtgcttttc aagaacctta ccccggtttt gaccgggctc ggagtttcga gcatcataat
     2101 aggtctggcg atcaaggagc ctctggaaaa cttcatctgc gggattctca ttatgctcaa
     2161 taaactcata atagaaggag aggccgtgga gatctccggc cagtcaggct cgatcgtcga
     2221 gatcaagttg aatcatgtac ttctgaaaac gtgggacgga aagatgataa ccatgcccag
     2281 ccgatcggtc tggacggcaa cgatagtcca cttctggccg gagaatataa ggcggaacga
     2341 tcttaacgtc ggtgtttctt attccagcga tttgaacaag gtcatgaagg tactcgagga
     2401 atcggtcaat tcgtatgaga agctctacaa agacgaaagc cataagccgg tgatccagtt
     2461 cacaggatac ggcagctcgt cgatagattt cgtgg
//
LOCUS       DAOOLY010000320         2477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_52261_length_2477_cov_3.255987, whole genome shotgun sequence.
ACCESSION   DAOOLY010000320 DAOOLY010000000
VERSION     DAOOLY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52261_length_2477_cov_3.255987"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLD15_11625"
     CDS             complement(<1..746)
                     /locus_tag="PLD15_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HPB64607.1"
                     /translation="MPEKKTLRIFISYGHDEHAELAVRIKNDLEERGHEVWFDRDRIK
                     PGKDFELYIEEGLKWLTEDKSGARFLYLMTPHSVRRPNGFCLNELTSAMMKELSIFPV
                     MVQMSEPPLSICRIQWLDMQECFPSCENTPYSVKFERLLAALEDRNWDFEGFEKNLLK
                     VFNPIDFGPDMLRHLKDFTGREWLKKEIEEWLEGKRPGNKQVFWIKGKPGIGKTAISS
                     WLVCTMYEVVAVHFFRSDSTEKRDPVRFIHT"
     gene            complement(764..>2477)
                     /locus_tag="PLD15_11630"
     CDS             complement(764..>2477)
                     /locus_tag="PLD15_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPB64608.1"
                     /translation="WLVLAVIAAFLFVLGFIGWIEFFDIAGKSVSIFSLFQLTFGLFI
                     RETFFPGFADILNLADYRIPLFLDIARIVAPWITLSVFITLVLSLLRNLTTVLAIRLF
                     YRNHIVVISDDVNSNYLLNCVRLDFLDSKIVLLKDTKVQDFSCRRFEDIPVISGSIED
                     PSILKITAINRAKHIVIATNDDSVNVRLLRSIEHTLSRKRKKKNIEIWLQIEDFKSFE
                     TFKGYKGEDPSLGTDVHIFSVFQRIAADTVDRFSPDRYDTTGGSQREIAVLGLDTLGQ
                     WLVLEAAQMYHFANMKKLRVTIIDSEIENKVKSLLRICPLLKKVIEINPVELLDFLQM
                     DSPESFSGVSTCFIAWPKFEEIEYISRKMRQLFFNSRELLDSPAIVLVDISSCLCSEI
                     MKESLETLEAIGVTVSRPAGISIKNEEECDCLAMQIHNVYSNLSQKELELEWSNMNDY
                     FKDENRYAARHLPIKLRSLGLEAVPTDDPREAVDFKEILERSEQLLAQLEHNRWLARK
                     LVNGYIHGKKLERKLRDRLKIHVDIRPWEELSEKDREKDLVVLRNIENMLREIGKKIV
                     PIKY"
BASE COUNT          581 a          628 c          556 g          712 t
ORIGIN      
        1 gtgtgtataa acctcaccgg atctctcttc tctgtggagt cgcttctgaa gaagtgaacg
       61 gcaacgacct cgtacatggt gcacacgagc catgagctta tcgccgtctt tcctataccc
      121 ggcttcccct ttatccagaa gacctgtttg tttcccggtc tctttccttc aagccattct
      181 tctatctctt tcttgagcca ttctcttccg gtgaagtctt tcaggtgtct caacatatcc
      241 ggtccgaaat ctatggggtt gaacactttc aagaggttct tctcaaaacc ttcgaagtcc
      301 caattcctgt cttcgagtgc ggccaggagt ctttcgaact tcacggaata gggggtattc
      361 tcacatgaag ggaagcactc ctgcatatcg agccactgta ttctgcagat ggagagggga
      421 ggttcgctca tctgaaccat aacagggaag atggagagct ctttcatcat ggcggaagtg
      481 agttcgttca ggcagaagcc gtttggcctt ctcacagaat gtggagtcat gagatagagg
      541 aatctcgctc cggatttatc ctctgtcagc cacttgagac cctcctctat gtaaagctcg
      601 aagtctttgc ccggcttaat cctgtccctg tcgaaccaga cctcatggcc tctctcttcg
      661 agatcgttct ttattcttac tgcgagctcg gcgtgttcgt catggccgta gcttatgaat
      721 atcctcagtg tctttttttc tggcatacct gcaccccctg taatcagtac tttatgggta
      781 cgatcttctt gccgatttcc cggagcatgt tttctatgtt tctcaggaca accaggtcct
      841 tttccctgtc tttttcgctc agttcttccc aggggcgtat atcgacgtgt atcttcaacc
      901 ggtctctcag ttttctttcc agcttttttc cgtgaatata tccgttgacg agctttctgg
      961 cgagccatct gttgtgttca agctgggcga gaagttgctc gctcctttca aggatctctt
     1021 tgaaatcaac cgcttcgcgt gggtcgtcgg tcgggaccgc ttccagaccg agcgacctca
     1081 gctttattgg cagatgcctt gcggcgtatc tgttctcgtc cttgaagtag tcgttcatat
     1141 tgctccattc tagctccagc tctttctgag agagattgga gtaaacgttg tgaatctgca
     1201 tcgccaggca gtcacattct tcctcgttct ttatggagat ccctgcaggt cttgagaccg
     1261 ttactcctat cgcttccagt gtctcgagcg attctttcat gatctcagaa catagacaag
     1321 acgagatatc cacaaggaca atcgcggggc tatctagaag ctctctcgag ttgaaaaaga
     1381 gctgccgcat cttcctggag atatactcta tctcttcaaa cttcggccag gcaatgaagc
     1441 aggtcgagac tcccgaaaaa gactccggac tatccatctg aagaaagtcg agcagctcaa
     1501 cgggatttat ctctatcact ttcttcaaca gggggcatat cctcaacagg cttttcactt
     1561 tgttttctat ctctgagtct ataattgtga ctctgagttt cttcatattc gcaaaatggt
     1621 acatctgggc ggcttcgaga accagccact ggccgagagt atccagtcca agcacggcta
     1681 tttccctttg agagccgcca gtcgtatcat accggtcggg actgaatcta tccaccgtat
     1741 cagcggctat acgctggaag acgctgaata tatggacatc ggtgcccagc gagggatctt
     1801 cacccttgta tcctttgaag gtctcgaaag atttgaagtc ctcaatctga agccagattt
     1861 cgatattctt cttctttctc ttcctggaga gtgtatgttc gatgcttctg agaagtcgga
     1921 catttaccga gtcgtcgttc gtagctatca ctatgtgttt tgccctatta atggccgtta
     1981 tcttcaggat ggagggatct tctatgctcc cggatataac aggaatatcc tcgaatctcc
     2041 tgcacgagaa atcctgaacc ttcgtatctt tgagcagcac gatcttgctg tcgagaaaat
     2101 ccagtctcac gcagttaaga agataattcg agttaacgtc atcgctgatt accacgatat
     2161 ggtttctgta aaagagcctt atggcaagaa cggtcgtgag atttctcaag agggaaagca
     2221 ccagtgtaat gaagaccgac agagttatcc atggggcgac tattcttgca atatccagaa
     2281 acaggggtat cctgtaatca gccagattca agatgtctgc aaaccccggg aaaaaagtct
     2341 cacgaataaa gagaccgaaa gtcaactgaa agagactgaa tatggaaacg cttttcccgg
     2401 ctatatcgaa gaactcgatc catcctatga agccgagtac aaagagaaag gctgcaataa
     2461 ccgccagaac caaccac
//
LOCUS       DAOOLY010000321         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_52456_length_2470_cov_3.756108, whole genome shotgun sequence.
ACCESSION   DAOOLY010000321 DAOOLY010000000
VERSION     DAOOLY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52456_length_2470_cov_3.756108"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLD15_11635"
     CDS             <1..941
                     /locus_tag="PLD15_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64609.1"
                     /translation="AIPVLLGVSVLAFLIISAAPGDFLDAYRMNPSITRDQLRLLERQ
                     FGLDQNVFVQYFKWLGNVLTGNFGYSFTYRVPVFNLVWRRLGATLLLSITTMIFTWGI
                     AIPLGIYSALHQYSLSDQTFSFLAFIGISIPNFFFALLWLFMSAKTGWFPIGGLISQN
                     FNDLSFFGKIGDYLWHVVGPMVTLGTSGLAGLMRQMRGQLLDQLRQDYVLFARAKGMP
                     EKNVIYKHAVRNAINPIVTIFGYALSGLLGGAVLTETVFGWPGMGRLVIEALNAQALF
                     LVMATLLLSAVLLVIGNLLADLMLAWVDPRIRFRLG"
     gene            955..>2470
                     /locus_tag="PLD15_11640"
     CDS             955..>2470
                     /locus_tag="PLD15_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64610.1"
                     /translation="MKKEKKNDLVTATPQEVKKDELFEIKYMGRWQLAWRALKKHKLG
                     LFSLWVLIILYLVAIFADFLAPNNPYDQRQDLSYAPPSKVHWTDEDGKLTAPYIYAWV
                     LERDPETYRTFFTEGISLGSVVAIDKTTGEEKTFEQGKDGVLNIYVTTRTIRYALDAD
                     GNRANLGGPEYSTVQTVKLTELDMLKETVVTETSNRTTLGALAPSRFRELLRIGPPVK
                     VVTERSLHRIFAQKENEIKDVVVEAISIVNEVIDVDGGDLELAREFLAELEISPNLVD
                     VIQDPQVTDYVTKKYPIKFFTQSWEYKLLGFIPMKLHLVGTELPSKFLLFGSDMYGRD
                     VFSRILFGSRVSMSIGLLAILITFSLGLSIGGAAGYFGGITDEILMRVTEILMSIPSF
                     YLLISLRAVLPTSIPPHITYILIVLILSFIGWPGMSRVIRGMVLSYKETEFVQAAIAM
                     GYPSGRVILRHIIPNTATYIIVSATLSIPGYILGEAGLSFLGLGITEPSASWGLM"
BASE COUNT          599 a          600 c          642 g          629 t
ORIGIN      
        1 tggcgattcc cgttttgctc ggggtttcgg ttctagcctt cttgatcatc tccgccgctc
       61 cgggtgattt tctggatgcc tatagaatga atccttctat caccagagat cagctgaggc
      121 ttctggagag acagttcggg ctcgatcaga acgtcttcgt tcagtatttc aaatggcttg
      181 gaaatgtctt gacgggaaat tttggatact cgttcaccta cagagtgccc gttttcaatc
      241 tggtgtggag gagactcggt gctacacttc ttctcagcat aaccacaatg atcttcacct
      301 ggggaatcgc catacctctg ggtatatatt cggcgcttca ccagtactcg ttgagcgacc
      361 agaccttctc cttcctcgcg ttcataggta tctccattcc aaacttcttt ttcgccttgc
      421 tatggctttt tatgtcggcc aaaaccggct ggtttcccat agggggcttg atatcgcaaa
      481 acttcaacga tctcagcttc ttcgggaaga taggagatta cctgtggcac gttgtcgggc
      541 cgatggttac tctcggtaca tctgggctgg cagggctcat gagacagatg cgcggacagc
      601 tcctggacca gctgaggcag gattacgtgc ttttcgccag ggcgaagggc atgcctgaga
      661 agaacgttat atacaagcac gccgttagaa acgcgataaa cccgatagtg acaatatttg
      721 gctacgcact ttccggactc ctcggcggcg ccgttctcac agagacggtc ttcggctggc
      781 ctggtatggg taggcttgtc atagaggctt tgaacgctca ggctctattc ctggtcatgg
      841 ctacgcttct tctgtcggcc gttctcctgg ttataggtaa tcttctggcc gatcttatgc
      901 ttgcctgggt cgatccgaga ataaggttca ggttaggctg aggggtgagt tggagtgaaa
      961 aaggaaaaga agaacgatct ggtaacggcg actcctcagg aagtgaaaaa agacgaactc
     1021 ttcgaaatca aatacatggg acgctggcaa ctcgcatggc gcgccctcaa aaagcataaa
     1081 ctggggcttt tttcgctttg ggtactcata atactctatc tcgttgcgat tttcgcggat
     1141 tttctggctc ccaacaaccc ctacgatcag agacaggatc tttcgtacgc ccccccttcg
     1201 aaagtccact ggaccgacga ggatggaaaa ctgacggccc cgtacattta tgcctgggtg
     1261 cttgagaggg atccagagac ttacagaacc ttttttacgg agggaatctc tctaggatcc
     1321 gttgtcgcca tcgataagac aaccggcgag gagaagacct ttgaacaggg taaagatggc
     1381 gtattgaata tatacgtaac aaccagaacg atacgctacg cactcgatgc ggacggaaac
     1441 agggccaacc tcggaggtcc ggaatacagt acggttcaga ccgtcaaatt gaccgaactg
     1501 gatatgttga aagagactgt tgtcaccgaa acatccaaca ggacaacact cggcgcgctc
     1561 gccccgagca ggttcagaga gttgctgagg atcggcccac ctgtcaaggt tgtaacggag
     1621 aggtctttgc acaggatctt cgcccagaag gaaaatgaaa taaaagacgt cgtagtagaa
     1681 gcaataagca tagtaaatga agtgatagac gtcgatggtg gagacctaga actggccaga
     1741 gagtttctcg cggaactaga aatctcgcca aacctcgtgg atgtgattca agacccccag
     1801 gtcacagatt atgtgaccaa gaagtatccg atcaagttct tcacacagtc ctgggagtac
     1861 aagttactgg gattcatccc catgaaactt cacctggtgg gaacggaact gccttcgaag
     1921 tttcttcttt tcggttccga tatgtacggt agagacgttt tctccagaat actgttcggt
     1981 tcgagagtat cgatgagcat cgggcttctc gcgatcctta taaccttctc tctgggactt
     2041 tccataggcg gagcggccgg atacttcggt ggtatcaccg atgaaatact tatgagagtg
     2101 acggaaattc tgatgtcgat accgagtttc tacctgctga tctctctgag agccgttctt
     2161 ccgaccagca tacctccgca catcacatat attctcatag tccttatact gagtttcatc
     2221 ggctggccgg gaatgtcgag ggtgatcagg ggtatggttt tgagttacaa agaaacggag
     2281 ttcgtacagg cggccatagc tatgggttat ccctcgggaa gggttatact tcgtcacatc
     2341 ataccaaata ctgcgactta cataatcgta tcggccactc tatcgattcc agggtacata
     2401 ctgggagagg ccggcctgag cttcctgggt ctgggaataa cggaaccctc ggccagctgg
     2461 ggacttatgc
//
LOCUS       DAOOLY010000322         2461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_52769_length_2461_cov_3.615544, whole genome shotgun sequence.
ACCESSION   DAOOLY010000322 DAOOLY010000000
VERSION     DAOOLY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52769_length_2461_cov_3.615544"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLD15_11645"
     CDS             complement(<1..926)
                     /locus_tag="PLD15_11645"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPB64611.1"
                     /translation="MKKLLKVSALMLLGLLTVVFFGAFEGGSQTVRNPTDAVLFPHDR
                     VIDVYIEVDEAELQKMFDQARMEEYIVCNVVYNGYRIKNVGIRPKGNSSLMQAANSTS
                     NRYSFKLDFNHYVKGQNLFGITKINLNNGFSDPSFMREYLTYEISEMLGLETPRTTYI
                     ALHINNQYFGLYLGVENIDENFVRDHFAYGYGDLYKPEGTGANLVYIDDDPQSYPGLA
                     LQTNEETSDRISIVEMLKVLQEGTYEELEKVLDIDSFLKYYALCVAAGSMDSILGGMS
                     HNYYLYDRDGQFVMLPWDFNMSFGGFGGFGGD"
     gene            complement(939..1619)
                     /locus_tag="PLD15_11650"
     CDS             complement(939..1619)
                     /locus_tag="PLD15_11650"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPB64612.1"
                     /translation="METFKDIFKNSFLENFSASQISAADTAITLLLTFLIGIFIFQVY
                     KRTFQGIMYTKSFNVSLVMIAMVTSLVIMAVTTNIVLSLGMVGALSIVRFRTAIKDPM
                     DIVFMFWAIAVGIVTGASFYLLAVMGSVIIGIILLVFANFKSQTSPYILLLDYVNSEA
                     EKAILDKLKDKVKRYNIKSKTVTAENIELSVELRIKSGEIDFVNELKSIDSVSNAVLI
                     SYNGDYAA"
     gene            complement(1637..1777)
                     /locus_tag="PLD15_11655"
     CDS             complement(1637..1777)
                     /locus_tag="PLD15_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64613.1"
                     /translation="MSDLMILEIKYDGFFPSHIKSIVQSEGRFRSSASKYVIGRKYNY
                     LY"
     gene            complement(1858..2328)
                     /locus_tag="PLD15_11660"
     CDS             complement(1858..2328)
                     /locus_tag="PLD15_11660"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPB64614.1"
                     /translation="MEDIRGRHELKYYISYFDFLSLKSKIRHVMEVDRNGNGNGDYEI
                     ISLYFDDIYNSAYREKISGTDNRTKYRIRIYNFSDSVIKLEKKTRKGDLTQKVSRVIS
                     RDEYEEIMKGRVSFLLEERSHEFIDFYEGIKNRTLKPKVLVRYNLRPTVSKRPT"
BASE COUNT          569 a          588 c          577 g          727 t
ORIGIN      
        1 ctgtcgccgc cgaatccccc gaatcccccg aaggacatgt tgaaatccca tggtagcata
       61 acgaattgac cgtctctgtc atagaggtag tagttgtggc tcattccgcc cagtatcgaa
      121 tccatgcttc cggcggctac gcaaagagcg taatacttga gaaaggagtc tatgtccagc
      181 actttttcaa gttcttcgta cgtcccttcc tgtaaaacct tcaacatctc aactatagag
      241 attctgtcgc tcgtctcctc gttggtttga agggccagac cggggtagct ctgcgggtcg
      301 tcgtctatgt acaccagatt ggctccggtg ccttccggtt tgtacagatc tccgtaaccg
      361 taagcgaagt gatcgcgtac gaagttttcg tctatgtttt cgacacccag ataaagcccg
      421 aagtactggt tgtttatatg caacgcaata taggttgtcc gcggagtttc gagaccgagc
      481 atttccgaga tctcgtacgt caggtactct ctcatgaacg aggggtctga gaaaccgttg
      541 ttgaggttta tcttggttat tccgaaaagg ttttgacctt tgacatagtg gttgaagtcg
      601 agcttgaagc tgtaacggtt cgaagtggaa ttcgctgcct gcatgagcga ggagttgccc
      661 ttgggtctga tcccgacgtt ttttatgcga tagccgttgt aaacgacgtt gcacacgatg
      721 tattcttcca tacgggcctg atcgaacatc ttctggagct cggcctcgtc aacttctata
      781 tatacgtcta taactctgtc gtgaggaaaa agaacggcgt ccgtcggatt tcttacagtc
      841 tgcgaacctc cctcgaaggc gccgaagaag accaccgtca ataagccaag caacattagt
      901 gccgacacct ttaaaagctt tttcatatca tcgcccctct aggcagcgta atcaccgttg
      961 taacttatta gaaccgcgtt cgatacgctg tcgatagatt tgagttcatt gacgaaatcg
     1021 atttctccgc ttttgatgcg caattccacg gaaagctcga tgttttccgc cgtgaccgtt
     1081 ttcgacttta tattgtacct tttgactttg tccttcagct tgtcgagtat cgccttctcg
     1141 gcctctgaat ttacgtagtc cagcagaagt atgtagggcg acgtctggga cttgaagttg
     1201 gcgaatacaa gcagtattat gcctatgatg accgagccca tgaccgcgag cagatagaaa
     1261 gaggcgcccg tgactatccc cacggcgatc gcccagaaca tgaagactat gtccatcggg
     1321 tctttaatcg cggttctgaa cctcacgatg cttagggcgc ccaccattcc aagcgaaagc
     1381 acgatgttgg tcgtcacggc cattattacc agagaggtga ccatggctat catgaccagc
     1441 gatacgttga aactcttggt gtacattatt ccctggaagg tcctcttata tacctgaaag
     1501 atgaaaatac cgatgagaaa ggttaagagc agtgttatcg ccgtatcggc tgccgatatc
     1561 tgcgatgccg aaaagttttc cagaaaactg ttcttgaata tgtctttgaa tgtctccatg
     1621 cctgtcctcc ttaaaactaa tacaggtagt tgtattttct cccgattacg tatttggatg
     1681 cgctgctcct gaacctcccc tcgctctgaa cgatactctt tatgtgagaa gggaagaatc
     1741 cgtcgtactt gatctcgagt atcatgagat cgctcatgga gcgtgtgaag gaaccgtttc
     1801 tgtcgaagag atcgaggttg ttgttgcccg tcgatagatt cccgtcgaag gtgattctca
     1861 ggttggccgc tttgagacag taggcctcag attgtagcgg accagtactt taggcttcaa
     1921 agttctgttt tttattcctt cgtagaagtc tatgaattcg tgtgatctct cctccagcaa
     1981 gaaggaaacc ctccctttca ttatctcttc gtattcgtct ctggaaatga cgcggctgac
     2041 cttttgagtc agatctccct tcctggtctt cttctccagc ttgataacgc tatcgctgaa
     2101 gttgtagatc cgtatcctgt atttcgttct gttgtctgtt ccgctgatct tctcgcggta
     2161 cgccgagttg tagatgtcgt cgaaatagag actgattatt tcatagtcgc cgttaccgtt
     2221 accgtttcga tcgacctcca tgacgtgtct tatcttgctc ttgagagaga ggaaatcgaa
     2281 atagcttatg taatacttga gttcgtgcct tcctctgata tcttccatgc aattcctcct
     2341 gttcattgtc gatcgatttt agagagcctc tgtcacagaa ttatcacaga gccggtgtga
     2401 ttgatttgtg acattttgat attagaataa agccatactg gacaggaggt tgtaagttga
     2461 a
//
LOCUS       DAOOLY010000323         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_53059_length_2452_cov_3.717146, whole genome shotgun sequence.
ACCESSION   DAOOLY010000323 DAOOLY010000000
VERSION     DAOOLY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53059_length_2452_cov_3.717146"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..267)
                     /gene="infA"
                     /locus_tag="PLD15_11665"
     CDS             complement(22..267)
                     /gene="infA"
                     /locus_tag="PLD15_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPB64615.1"
                     /translation="MSDKSDIIKMDGTVLESMPNATFKVELENGHTVLAHISGKMRKN
                     FIRLIPGDRVVVEVSIYDLTKGRIVYRKRIDKGGETN"
     gene            complement(280..1041)
                     /gene="map"
                     /locus_tag="PLD15_11670"
     CDS             complement(280..1041)
                     /gene="map"
                     /locus_tag="PLD15_11670"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB64616.1"
                     /translation="MFDMVRLKSPEEIGKIEVAAKIVAEVLSTLSEYASVGATAHDME
                     RAATELIAKRGGTPAFKGYGGYPYTLCVSVNEEVIHGFPLKEKVFVAGDIVSVDCGVV
                     KDGYYGDAAKTFVVGEFLNEKDRLLLLKTEESLYEGIRMAVAGNRLGDIGNAVQTCVE
                     SAGFSVIRDYVGHGVGTSLHEDPQVANYGRKGSGMLLRVGMTLAIEPMVASGHYDVEL
                     LEDGWTAVTADGSRAAHFEHDIAIMEDGPRILSRL"
     gene            complement(1034..1684)
                     /locus_tag="PLD15_11675"
     CDS             complement(1034..1684)
                     /locus_tag="PLD15_11675"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPB64617.1"
                     /translation="MNIVLMGPPGAGKGTQAKRIAHKYNIPHISTGDMLREAVAAGND
                     LGLKVGEIMEKGLLVPDTLMIGLVKERLSRGDARNGFILDGFPRTVEQAGALDEMLEE
                     LDRKVDVVLLVNSSEEVVVDRISSRRVCPKCGKVYNLLTIKPHIDGRCDVDGSQLVQR
                     DDDLAETVRSRYRVYVEKTAPVIDYYSNSEVFFLDIDGSGNIDEVAGEIFGLLGNV"
     gene            complement(1687..>2452)
                     /locus_tag="PLD15_11680"
     CDS             complement(1687..>2452)
                     /locus_tag="PLD15_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HPB64618.1"
                     /translation="IGNGISILIFGGIVARYPASIAEIVVSLSPFQWAILLAIALFTV
                     VAVIYVQMGERRIEVQYARRVTGRRVYGGVSTHIPIKVNQGGVIPIIFSSAIMMLPQF
                     IATAFPAGSSGRSVMEMLFAQTSPLYILLYGGMVFFFTFFYSSLVFDVREVSDNIKNY
                     GGYIPGIRPGFSTQQYIQRVLNRVVFMGAVFLVVIALLPLVMGGIFGIRGLAIGGTST
                     LIAVGVAIDILQQMETHLMVRHYEGFVKKGKLRGRR"
BASE COUNT          577 a          700 c          534 g          641 t
ORIGIN      
        1 tcttgaccga agcgcggact ttcaattcgt ttctcctcct ttgtctatcc tttttcgata
       61 aacaattctt cctttggtaa gatcgtagat cgaaacctcc actacgaccc tatcgccggg
      121 gatcaacctg atgaagttct tgcgcatttt tccggagatg tgagcgagaa cggtgtgtcc
      181 attttccagt tcgaccttga aggttgcgtt gggcatagat tccagcaccg ttccatccat
      241 ctttatgata tcgcttttgt ccgacatcag tgatcctcct cacagtctcg aaagaattct
      301 gggaccgtct tccattatgg ctatgtcgtg ctcgaagtgt gccgcacgcg aaccatcggc
      361 cgtaacggct gtccatccat cttccaggag ttccacatcg taatggccac tggcgaccat
      421 tggttcgatc gcaagcgtca ttccgaccct caacaacatc ccgctgccct ttcggccgta
      481 attggcgacc tgtggatcct catggaggct cgtacccact ccatgtccta catagtccct
      541 gattacggaa aagccagcac tttcaacaca ggtttgaacg gcgttgccga tatcgcccag
      601 gcggttgccg gccactgcca ttcttattcc ttcgtataat gactcctccg ttttcaggag
      661 aagtaatcta tctttctcgt tcaaaaactc acccacaacg aaagtctttg cggcatcacc
      721 gtagtagccg tccttcacga ctccgcaatc gacagatact atatcgccgg ccacgaagac
      781 cttctccttc aaggggaacc cgtgaataac ttcttcgttg accgatacac acagagtgta
      841 cgggtatcca ccgtatccct tgaaagctgg cgtgccgcct ctttttgcta tcaactcggt
      901 cgccgcccgt tccatatcgt gggcggttgc tccaaccgat gcgtattctg aaagagtgga
      961 aaggacttct gcaacgattt tggcagcaac ttcgatttta cctatttcct ctggagattt
     1021 caacctgacc atatcaaaca ttccccagaa gcccgaaaat ctctccggcc acttcatcaa
     1081 tattgcctga accatcgata tcgagaaaga agacttcact gttggaataa tagtcgatca
     1141 cgggggctgt tttttcgacg tagacacggt atctggacct tacagtctcc gcaagatcgt
     1201 catctctctg taccaattgc gagccgtcga catcgcaccg cccatcgata tgtggtttga
     1261 tagtgagtaa attatagacc ttgccgcact tcggacagac ccttctgctg gaaatcctgt
     1321 caaccaccac ttcttcgctg gaattcacca aaagcacaac atcgactttc ctgtcgagct
     1381 cttcaagcat ctcatccaaa gctccggcct gttcgacagt ccttggaaat ccgtcaagta
     1441 tgaatccatt cctcgcgtcc cctctggata atctttcctt aaccagacct atcataaggg
     1501 tatcgggaac gagcagccct ttttccataa tctctccgac cttcagccca aggtcgttgc
     1561 cagcagccac ggcttccctc aacatatcgc ctgtggatat gtgcggaata ttgtatttat
     1621 gggctattcg ctttgcctgt gtccccttac cggcaccggg aggacccatc agaacgatat
     1681 tcattcttac ctccttccac ggagtttgcc tttcttgaca aatccctcat agtgtctgac
     1741 catgaggtgc gtttccatct gctgtagtat atcgatggcg acaccgacgg cgatcaatgt
     1801 ggatgttccg cctatcgcca gacctcttat tccaaaaatg ccacccatta ccagaggcag
     1861 taaggcgatc acgaccagaa acacggctcc catgaaaacg acgcggttca acactctctg
     1921 tatgtattgc tgggtagaga agcccggtct tattccgggg atgtatcctc cgtagttctt
     1981 gatattatct gaaacttccc tgacgtcgaa aacgagcgag ctgtagaaga aggtgaagaa
     2041 gaacaccatt ccaccgtaga ggagtatata gagcggcgag gtctgagcga atagcatctc
     2101 catcacgctt ctaccgctac ttccggccgg gaaagcggtg gctatgaact ggggtaacat
     2161 cattatagcc gaactgaaaa ttatcgggat gactccacct tgattgacct ttatcgggat
     2221 atgcgtggag actccgccgt acactcttct gcccgtcact ctcctggcgt actgaacctc
     2281 gatccttctc tcgcccatct ggacgtagat tacggccaca acggtgaaga gggcgatcgc
     2341 cagaagtatc gcccactgga agggcgacag ggatacaacg atctctgcta tagaggcggg
     2401 gtaccttgca actataccac cgaagatcaa tatcgagata ccgtttccaa tt
//
LOCUS       DAOOLY010000324         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_53520_length_2437_cov_3.588581, whole genome shotgun sequence.
ACCESSION   DAOOLY010000324 DAOOLY010000000
VERSION     DAOOLY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53520_length_2437_cov_3.588581"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLD15_11685"
     CDS             <1..968
                     /locus_tag="PLD15_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB64619.1"
                     /translation="YFYPKGGVGSLAESVYNKVLEFGGKIETGREVTEIQAGRKILLD
                     DMGGSYMYNELVWAADLKRLYSILKTEGLSEATVEKVDTFRSEISKKHGTDSIFSVFI
                     AVDEPPEAFEEISNGHFFYTPSRKGLGEIHRSQLRDLLKNWSGNSKEEVFGWLERFCE
                     LDTYEISIPVLKDRNSAPAGKTGLIISTLLEYEIVKKAHEAGWYSEFKEELQKRIIGV
                     LSNSIYPMLKDKVLFAFSSSPLSIERAVGSSEGSIVGWSFEEPIPVVSSMMKVTSSVK
                     TPIPGVLQAGKWTYSPSGVPMCILTGKLAAAEAIKERKSRENRIR"
     gene            1040..1588
                     /locus_tag="PLD15_11690"
     CDS             1040..1588
                     /locus_tag="PLD15_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB64620.1"
                     /translation="MSCEILLLGNPLLRERSSIVTEFESEETLRQIALLKKTLDEFRR
                     ENGFGRGIAAPQIGILRRIIALDLGKGSFVIVNPRIISVSGSKFTMWDDCMSFPDLLV
                     RLERYSSIDLKYEDEKGIEHEWMRLGQAESELLQHEIDHLDGVLAIDRAIDTSSIIYR
                     REYEKNREYFDHLVDYTIEPTV"
     gene            1811..>2437
                     /locus_tag="PLD15_11695"
     CDS             1811..>2437
                     /locus_tag="PLD15_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HPB64621.1"
                     /translation="MRRVSDDFGKGLNVSPEAALEFEIQGERLLKRVNELMSAREDIS
                     ELLGMNPLYIMYDNNSNHLRFISNVLKLNDYDLLARTLPWVYKTYTSRNFSEDFFPEV
                     LKTWMEAIREQLSLENSEQIIKVYEAMLSSHDLSVKTSEDALIGMNVDEQWKAIEQKV
                     VLALLKGSYRELQEIALGTIKDTRSLSDFYLKVVQPAMYEIGSLWQTGE"
BASE COUNT          686 a          517 c          653 g          581 t
ORIGIN      
        1 actacttcta cccgaagggt ggagtgggaa gtctggctga atcggtgtat aacaaagttc
       61 ttgaatttgg cgggaagata gagaccggaa gagaagtcac ggagatccaa gcgggcagaa
      121 agatactcct ggacgatatg ggaggatctt acatgtacaa tgaactcgtg tgggccgccg
      181 acctcaagag actctactcg attttgaaaa cggaaggtct gtccgaagca acagttgaaa
      241 aggtcgacac atttagatcc gagatatcga aaaagcacgg gaccgattcg attttcagcg
      301 tctttatcgc ggtggatgaa ccgcctgagg ctttcgagga gatttcaaac ggccacttct
      361 tctacactcc ttcgaggaag ggactcggcg agattcatcg cagccaattg agagacctcc
      421 tgaagaactg gtccgggaat tcgaaagagg aggtgttcgg ctggctcgaa agattctgtg
      481 aactggacac ctacgagata tctataccag tgctcaaaga tcgcaactcg gctccggccg
      541 gaaagacggg cctgataatc agcactctct tagaatacga gatcgtaaag aaggcacatg
      601 aagccggctg gtacagcgag ttcaaagaag agctccaaaa gaggattatt ggagttcttt
      661 caaattcgat ctacccgatg ttgaaggata aagtcctctt cgcgttttcc agcagccctc
      721 tttcaatcga aagagcggtg ggcagctccg aagggtctat cgtcggttgg tctttcgaag
      781 agccgatacc tgtagtatct agcatgatga aagtcaccag ctcagtaaaa acacccatac
      841 caggtgttct tcaggccgga aagtggacct acagcccttc cggtgttccg atgtgtatat
      901 tgaccggtaa gcttgctgcc gcagaagcaa ttaaagaaag aaaatcaaga gagaatcgta
      961 ttagataggg cagtttaatg tcaataggaa taattatgtg tatggaattg aatgatatat
     1021 aaggtcggga aggtgaaata tgtcgtgtga aatcctgttg cttggaaatc ctttgctccg
     1081 ggagaggtct tcaatagtca cggaatttga gagcgaagaa acgttgaggc agatcgccct
     1141 gctcaagaag actctcgatg aattcagacg ggagaacggc ttcggccgcg gtatcgccgc
     1201 accacagatc ggaatactga ggagaataat cgcgctcgat ctcgggaagg gtagcttcgt
     1261 aatcgtgaat ccgcgaatta taagcgtcag cggatctaaa ttcaccatgt gggacgactg
     1321 catgtccttt cccgatctgc tggtccgtct agaaagatac agttcaatag atttgaagta
     1381 cgaggacgag aaaggaatcg aacatgaatg gatgaggctg ggccaggcag agtccgagct
     1441 actgcagcat gagatagatc acctcgacgg tgttctggcc atcgatcgcg cgatcgacac
     1501 ctcgagcatc atctaccgcc gggaatacga aaagaaccgt gagtacttcg accacctcgt
     1561 tgactacact atcgaaccga cagtttaact cttgcgtttt gaattgaggc aactggggag
     1621 ggcttagagc accataggtg agattcccga acagatccga cacgacggga agataaggtt
     1681 gtacaattcc gttctatatt tacgttgatt cgaaaccgca gcaaattgca tgaggataat
     1741 ttttcatagt ttataatgat tatgcggtgt gagataggaa tcttaccggg ccatttcagg
     1801 gggtgttttt atgcggcgag tgtcagatga ttttggaaag ggtctgaacg tttcacctga
     1861 ggctgctctg gagtttgaga ttcagggtga aaggttgctg aagagagtca acgaactaat
     1921 gtccgctaga gaggatatat cggagctttt gggtatgaat ccgctctata taatgtacga
     1981 caacaattcg aaccatctgc gctttatctc gaacgtcttg aagctcaacg attatgacct
     2041 gcttgccagg acgctcccct gggtttacaa gacctatacc tctagaaact tctcggaaga
     2101 tttctttccg gaagtcttga agacctggat ggaagcgata agagagcaac tctctctcga
     2161 aaattcggag caaattataa aagtgtatga ggcgatgctt tcctctcacg atttatcggt
     2221 gaagacttcg gaagatgctc tcatagggat gaatgtggat gagcagtgga aggcaataga
     2281 acaaaaggtg gttctcgcgc tgctgaaggg tagctaccgc gagcttcagg agatcgcact
     2341 cggcacgatc aaagatacga gatccctctc cgacttctac cttaaagtgg ttcaacccgc
     2401 gatgtacgaa atcgggtcgc tatggcaaac gggcgaa
//
LOCUS       DAOOLY010000325         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_53549_length_2436_cov_4.185216, whole genome shotgun sequence.
ACCESSION   DAOOLY010000325 DAOOLY010000000
VERSION     DAOOLY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53549_length_2436_cov_4.185216"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLD15_11700"
     CDS             complement(<1..795)
                     /locus_tag="PLD15_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPB64622.1"
                     /translation="MYKVISTEAIAPDTFDIWLEHPDIDKRAKPGQFLILRLDEFSER
                     IPLTIVSTAEGRVRVIIKAIGKSTYKLCAVKPGDTIADIVGPLGNPSEIDRFGTVCVI
                     GGGVGIAPLFPIARALKEAGNRVVGILGAAKKELLIMKDEFLPYLDELYITTDDGSEG
                     IKGNVTVALQEVLKKEKVEFIWAIGPMVMMKFVSLMAAENDILCWVSLNPIMVDGTGM
                     CGACRAEVGEEVKFACVHGPEFDGRKDKWDELIKRQYQYREEEKQAM"
     gene            1058..2281
                     /locus_tag="PLD15_11705"
     CDS             1058..2281
                     /locus_tag="PLD15_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPB64623.1"
                     /translation="MANLNEVKAANKHLVLDLVRRKEVISRADLSRLSGLTRATVSAL
                     TGELIEERFLKELMVGDSKKGRGGRKPVLLSLDGEGKLFAGVDLHWDYFQIAVSNILG
                     EIVTEDVYRFDRRLSPEEYFGVLSERANRVFHKDDFSGRISAIGVSLYGVVDTKNGVL
                     KFPSHMHWPQIELERYIAGFPAPVFWESRSAASLIAETWFHEELYPKDGLIVFVNVIE
                     GIGGAVMLNGEILKNDGISTGEIGHTTVDINGNTCTCGRRGCLEAYVSDKKLVESYVD
                     QLDKLSLPTKSIRDVAIKIAQMASRGEEPAVKAVQQMARNLSVGIGNVINFLSPNYVI
                     IGGFITEAWSVVEPIIREEVTRQALPGLFISTEVVKNHYGSRSGLIGALAVAIREKMR
                     QVGSIEFNSLEHHSV"
BASE COUNT          613 a          586 c          631 g          606 t
ORIGIN      
        1 catggcctgt ttctcttctt ctctgtactg gtactgcctt ttgatcagct cgtcccattt
       61 gtcctttctt ccgtcgaact ccggcccgtg tacgcaggcg aacttgacct cttcccccac
      121 ttccgctctg catgctccgc acatgcctgt gccgtcgacc attatcgggt tgagcgatac
      181 ccagcacagg atatcatttt cggccgccat gagcgatacg aatttcatca ttaccatggg
      241 tccaatagcc cagataaatt cgactttctc ttttttcaag acttcctgca aagcgactgt
      301 gacattacct ttgattcctt ccgaaccgtc atcggtagtg atgtaaagct catccagata
      361 aggaaggaat tcatctttca tgatcagcag ttcttttttc gcggctccga ggatacccac
      421 caccctgttt cctgcctcct taagcgccct ggcgattgga aagagcggcg ctattccaac
      481 tcctcctccg attacacaaa cagtcccgaa gcgatctatc tcactcgggt taccgagggg
      541 ccccactatg tcggcgatcg tgtctccggg cttgacggca cagagcttat acgtgctctt
      601 cccgatggcc tttataatga ctcttactct cccttcggct gtcgatacga tagttaaggg
      661 tattctctca ctgaactcgt ctagccttaa aataaggaac tgtcccggct tggccctttt
      721 gtcgatatcg ggatgctcaa gccagatgtc gaaagtatct ggagcaatag cctctgtaga
      781 gatcactttg tacatctcat cacctcaaaa gtatgataac agaattattc ttgagatatt
      841 ctgaaggata tatgaaagta agtcttacac atcaaattac cgatagggtt tcataggagg
      901 aagcagctcc ggcgcgacta ctaacaataa tcgctcttaa tcgcttatag ttcacaggaa
      961 agcgacgaga acagactcgt gcgtacttgg gatagactca atcgatgaga tattatttgt
     1021 ttagttacta accaaacaaa caattggtgg tggtgagttg gccaatctga atgaagtaaa
     1081 agccgcgaac aaacacctcg ttctggatct tgtgagaagg aaagaggtaa tttcgagggc
     1141 cgatctttcg aggctttcgg gactcaccag ggcaaccgtg tcggctctta ccggagaact
     1201 aatcgaggag aggtttctca aagagctcat ggtaggcgac tcgaaaaaag gcaggggcgg
     1261 tcgaaaaccc gttttactga gtctggacgg tgaaggcaag ctctttgcgg gggtcgatct
     1321 tcactgggat tactttcaga tcgctgtgtc aaacatactg ggcgagatcg tcacggagga
     1381 tgtgtaccgc ttcgacagga gactctctcc ggaagaatat ttcggggtgt tgagcgaaag
     1441 ggcaaacaga gtctttcaca aagatgattt ctccgggagg atatcggcga taggcgtcag
     1501 tctctacggt gtcgtagata caaaaaatgg agtgcttaaa tttcccagcc atatgcactg
     1561 gccgcagatt gaactggagc gatacatagc gggttttcca gccccggtct tctgggaaag
     1621 ccgttcggca gcctccctga tcgcggagac ctggttccac gaggagctat atcccaagga
     1681 cggcctgata gtattcgtga atgtgataga aggtatcggt ggtgcggtga tgctcaacgg
     1741 agagattctg aagaacgatg ggataagcac cggagagatc ggtcatacca ccgttgatat
     1801 taatggcaat acatgcacct gtgggaggcg cggttgtctt gaggcttacg tgtccgacaa
     1861 gaagcttgtg gagagctatg tggatcaact ggacaagctc agccttccga ccaaaagtat
     1921 aagggatgtg gccataaaga tagcgcagat ggcctcccgc ggagaagaac cggctgtgaa
     1981 agccgtacag cagatggcga gaaatctgag tgtcgggatc gggaacgtta taaattttct
     2041 cagcccgaat tacgtgatca tcgggggctt cataaccgag gcctggtcag tcgtagaacc
     2101 gataatacgc gaagaggtaa ccagacaggc cctgccggga ctcttcattt cgaccgaggt
     2161 tgtgaaaaac cattacggtt cccgctcggg ccttatcggg gcactggccg tcgccataag
     2221 agaaaagatg cggcaggtcg gttcaatcga gtttaattcg ctggaacacc atagcgtttg
     2281 acaggtcgat cgaaagtgtg cttggagttg tataaaggag gagaccataa atggcatcgc
     2341 tttccagttc ggatcggcaa cagttgagct tcactcttgt aggtgacgta cacatgggtc
     2401 caagcaattc tacgagaccg ggcgaagaga cacccg
//
LOCUS       DAOOLY010000326         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_53675_length_2432_cov_4.216239, whole genome shotgun sequence.
ACCESSION   DAOOLY010000326 DAOOLY010000000
VERSION     DAOOLY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53675_length_2432_cov_4.216239"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLD15_11710"
     CDS             <1..617
                     /locus_tag="PLD15_11710"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPB64624.1"
                     /translation="SGKRKLLLQFVFASIVVLMIQLYNPHTSMKIPFGGEIEFGFLYY
                     IISAIVLAGMSNAVNLSDGIDGLAGSMFIFSLIPLFALPLWQNGIIAPICGALAGFLW
                     HNWFPASVFMGDTGALGLGGMLATVFAIEGREIFLIFFGVMFIIEMFSVILQVGSFKL
                     FGRRIFRMSPIHHHFELKGWKETKITFRFSLLALTAAVVGLLAW"
     gene            627..1961
                     /gene="murD"
                     /locus_tag="PLD15_11715"
     CDS             627..1961
                     /gene="murD"
                     /locus_tag="PLD15_11715"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPB64625.1"
                     /translation="MLKSKMGFVGFGISNYRLLRVLGKEYPSSSFFVSDMGSIEGEPM
                     EFLKKNDIEFEDNGHTERLMECDSFVVSPGVSPFSKVGRAILESEKLFTTELEVSLDI
                     LKSKPHGVIIGITGTNGKSTTVTMLSHVLAARGKTVFQGGNLGDPLAGILGETLEYYV
                     LEVSSFQLKWFSRSDPRFHLSAVINIGEDHIDYHTTYEDYRQSKLRLVDMTEGFSLLP
                     VCEKDFFNGKPQQGKILPFSMHGKTDYCYDMGKLRVDGESFDSGELPYEGIHNFEDTL
                     VVMALANLLGMPMRGVFEDLKKYEFLSHRLQLVGEIDGVKYYDDSKATNAHAVVAALK
                     NFNPEKTVLVLGGKEKDETYIELLQVLKGLKGIIVLGNSMKSLIQKMRTSGINFQLAG
                     SMEEAVETGRRNSCSGDSVVLSPAGSSYDLYRNYKERGDHFKSIVNSLAVGK"
     gene            1961..>2432
                     /locus_tag="PLD15_11720"
     CDS             1961..>2432
                     /locus_tag="PLD15_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPB64626.1"
                     /translation="MKKTYLMLALFTSIFLIFGFVFIYSAGISMEARHPDVSASEFLE
                     KQLIAFAIGLAGALVMIYLKGSWHFKKAFTIYYPLTLILLVAVLFFPDRGGSHRWLDI
                     GSLSLQISEFAKISLLMVLASYLGNIKKRSFLNTFLIPIGLVLPVAFLVFIEPDL"
BASE COUNT          587 a          546 c          644 g          655 t
ORIGIN      
        1 tgagcggcaa gaggaagttg ctcctgcagt tcgtatttgc ttctatcgtg gtacttatga
       61 ttcagcttta caatccccat acatctatga agatcccctt cggtggcgag atagaattcg
      121 gttttctgta ttacataatc tccgcaatag ttcttgctgg catgagcaat gcagtcaatc
      181 tttccgacgg gatagatggg ctcgcgggtt ctatgttcat cttctcgcta atacctctct
      241 ttgcccttcc attgtggcag aatggaataa tcgccccgat ctgcggggct ctggccggtt
      301 tcttgtggca caactggttt ccggcatcag ttttcatggg agatacgggc gcgctcggtt
      361 tggggggtat gctggccacg gttttcgcga tcgaaggtag agagatcttc ctgatttttt
      421 tcggcgtcat gttcataatt gaaatgttca gtgtgatact tcaggtggga tccttcaagc
      481 tctttgggag aaggatattc aggatgtcgc ccattcatca ccactttgaa ctcaaaggct
      541 ggaaggaaac aaagatcact ttcagatttt cgttgcttgc attgaccgcg gccgtcgttg
      601 gtctgctggc ctggtaggtg gaggacgtgt tgaaaagcaa aatgggtttt gtggggtttg
      661 gaatatccaa ctatagattg ctcagggtgc tcggcaagga gtatccatct tcttctttct
      721 tcgtatcgga tatgggatcg atcgagggag aacccatgga atttctgaaa aaaaacgata
      781 tagagtttga agataacggt catacggaaa gacttatgga atgcgacagc ttcgtggtat
      841 caccgggagt ttcgccgttc tcgaaagtcg ggagggccat tctcgagagt gaaaagctct
      901 tcactaccga actggaagta tccctggata tcctgaaatc gaaacctcac ggggtgataa
      961 tcggaatcac aggaactaac ggaaaatcca ccacggtcac catgctcagc catgtgctcg
     1021 cggccagggg aaagacggtc tttcagggcg gaaatctggg cgatccgctg gcaggaatac
     1081 tgggagagac ccttgagtac tacgttctag aagtcagttc attccagctg aagtggttca
     1141 gcagaagcga tccccggttc catttgtcgg ccgtcatcaa catcggcgag gaccatatag
     1201 actaccatac aacgtacgaa gactaccgac agtcaaaact ccggctggtc gatatgacgg
     1261 agggcttttc cctgcttccc gtgtgcgaga aggacttttt taatggcaag cctcaacagg
     1321 gtaagatact ccccttttcg atgcacggca agaccgatta ctgttacgat atgggtaagt
     1381 tgagagtaga cggggagtct ttcgatagcg gggagctgcc ttatgaggga attcacaatt
     1441 ttgaagacac tctggtggtc atggctcttg caaatcttct gggtatgcct atgagaggag
     1501 tcttcgaaga tctcaaaaag tacgagttcc tctctcacag gcttcagctg gttggagaga
     1561 tagatggtgt aaaatattac gatgattcta aggcaaccaa cgcacacgcc gtagtggcgg
     1621 cgctgaagaa cttcaacccc gagaagaccg ttcttgtgct tggtgggaag gaaaaggatg
     1681 agacctacat cgagctgctc caggtcttaa aagggttgaa aggtattatc gtactcggca
     1741 actcgatgaa atctctgatc cagaagatga gaacgtcggg aataaacttc cagctggcag
     1801 gttccatgga agaagccgtg gagacaggta gacggaattc gtgcagcggc gacagtgtcg
     1861 ttctcagccc ggccggttcc agctacgatc tttacaggaa ttataaagag aggggagacc
     1921 atttcaagag catagtcaac tcgcttgcag tggggaagta atgaagaaga cttatttgat
     1981 gctcgctctt tttacaagta tcttcctgat tttcggattc gtttttatct acagtgccgg
     2041 aatcagcatg gaggcaaggc accccgacgt cagcgcttcg gaattcctgg agaagcagct
     2101 gatagctttc gcgataggtc tggccggcgc gctcgttatg atctatctga agggctcctg
     2161 gcatttcaag aaggccttca cgatttacta tcccctcacg cttattctcc tggtggcagt
     2221 actctttttc cccgacaggg gaggatctca caggtggctg gatatcggca gtctttccct
     2281 ccagatctcc gagttcgcca agatatctct gcttatggtt ctggccagtt atctgggaaa
     2341 tataaagaag agatctttcc taaatacctt tctgattcct atcggtctcg ttttgccggt
     2401 agcctttctt gttttcatag agcccgatct gt
//
LOCUS       DAOOLY010000327         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_53837_length_2427_cov_4.233558, whole genome shotgun sequence.
ACCESSION   DAOOLY010000327 DAOOLY010000000
VERSION     DAOOLY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53837_length_2427_cov_4.233558"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLD15_11725"
     CDS             <1..687
                     /locus_tag="PLD15_11725"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPB64627.1"
                     /translation="TMNANKAVKAFFSEEPPVTYTLTMLEPAGQGEVEPAVGNHQYIE
                     GTVVNLTATPAVGWDFDRWEVDGAFYSSNPTTTLTMNANKAVKAFFSEEPPVIYTLTM
                     LEPAGQGSVIPTVGTHLYDQGTNVNLLAMPEKGWDFEGWFIDGEFYSDNPSTSIIINA
                     DMTAMAVFAEEVDKVDLYMSLNGRGTVDPPEGGPYEYDLGTVVNISATPASNWRFVEW
                     VVDGMELFNR"
     gene            830..1780
                     /locus_tag="PLD15_11730"
     CDS             830..1780
                     /locus_tag="PLD15_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPB64628.1"
                     /translation="MRRKFLIMMVISSFLCAVSTFAAPGKVLTVGADGSQYEKIQEAI
                     DAAGAGDTVKILPGIYIENITINKNLTIVGTSKEEVVIRAASIDKPAVFFRGVSDFSM
                     ENLCVETVGAAINISRSSGRIIDSHVAGGRFGISFSGSGMTLKIIDSYITALAGAEND
                     GPIGTDLVGIYAYGEASLEIEKTTFERTGTGVSLSNGMNFLIKNSIFKRNTIGVALNG
                     TASGSLIENRVFENVENGVLLNITSSVILTDNIFYSNSGHGLDLYLIECTECGCGGTE
                     FNGTVTGSGNLFDSEEEICPIEYWEDGFFVIDRDLAVDRQ"
     gene            complement(1866..>2427)
                     /locus_tag="PLD15_11735"
     CDS             complement(1866..>2427)
                     /locus_tag="PLD15_11735"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492510.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HPB64629.1"
                     /translation="HNSVEEAVLCEVDPYVIEASRKYLKEMSIELDNPRATIVNENGA
                     DYVRKFKDRFDVIVVDSTDPTAGEGGHLFTKEFYVNCYAALRKDGVFCAETEDPFYDK
                     GWVAIAFNRISSVFPVCRMYTSFMTTYPSGMWTYTLGSKTFDPVKDFDEERVLDFNAP
                     LRYYNSEIHPAAFALPTFLRELTGQF"
BASE COUNT          622 a          564 c          658 g          583 t
ORIGIN      
        1 acgatgaacg ccaataaggc agtgaaggcc ttcttcagcg aagagccacc ggtgacgtac
       61 acactgacta tgcttgagcc tgccggacag ggagaggtcg agcctgctgt gggaaatcac
      121 caatacatag agggaacggt ggttaacctt acggccaccc cggccgtggg ctgggacttc
      181 gacaggtggg aggttgacgg ggctttctat tcaagcaatc cgactaccac cctaacgatg
      241 aacgccaata aggcagtgaa ggccttcttc agcgaagagc caccggtgat ctacactctt
      301 acgatgctgg aaccggccgg acaggggagt gtaattccca ccgtcgggac gcacctgtac
      361 gatcagggaa cgaatgtcaa cctactggcc atgcccgaaa aaggctggga ttttgaaggc
      421 tggtttatcg atggcgagtt ctattcagac aatccctcca cttctataat aataaacgca
      481 gatatgactg ccatggcggt ttttgcagaa gaggtggata aggtcgatct ttacatgagc
      541 ctgaacggtc ggggtacagt cgacccacct gaaggaggac catacgagta tgatctgggt
      601 acggtcgtga acatttcggc cactcccgca tccaactgga ggtttgttga atgggttgtt
      661 gacggtatgg aattattcaa ccgataaaaa caccaccctt ttgatggatt ctgacaaaga
      721 tgttctggcc gtcttccggt gcaactgtgg aaatgttaag ttcgagcaca tggaagagag
      781 gtaggtcatt gggtatcttt ttcggcaata gaatagagga ggtgggagag tgcgccgcaa
      841 attcctgatt atgatggtta tatcgtcttt tctctgtgcg gtgtccacat ttgccgcacc
      901 cggaaaagtc ctcaccgtcg gggcagacgg cagccagtat gagaagatcc aggaggcgat
      961 agatgccgcc ggggctggag acaccgtcaa aatacttccg ggaatataca tagagaacat
     1021 aacgataaat aaaaacctga caatagtggg aacttcgaaa gaagaagtgg ttataagggc
     1081 cgcgtcgatc gataaacctg ccgttttctt tcggggagtc tccgactttt cgatggagaa
     1141 cctctgtgtt gaaactgttg gagcggctat aaacatctct agatcttcgg gccggatcat
     1201 cgattcacac gttgccggag gcaggttcgg catatctttc agtggaagcg gaatgacact
     1261 caaaataata gactcataca taacggctct ggccggagct gagaatgacg gacccatcgg
     1321 tacggatctg gtgggtatat atgcctacgg tgaagcgtcg ctagagatcg aaaagacgac
     1381 ctttgaaagg accggaaccg gtgtaagcct ctccaacgga atgaatttct tgattaaaaa
     1441 cagcatcttc aaaaggaaca ccataggtgt cgcactaaac ggcacggcca gcggttcctt
     1501 gattgaaaac agggttttcg agaatgtcga aaacggggtg ctcttgaata taacttcttc
     1561 ggtgatcctt acagataaca ttttttacag caactcggga cacggcctgg atctgtatct
     1621 gatagagtgc acggaatgcg gttgtggagg aaccgaattc aacggtacgg tcacgggctc
     1681 tggtaacctt ttcgattcgg aagaggagat ctgcccgata gagtactggg aggacggttt
     1741 cttcgtcatc gacagggatt tggcggtgga tcgacagtaa cttctcgtgt ctgacctcga
     1801 ccctccgctc ttcacggtga atagctttta atgttcttcc agaggacggg ctcttctagg
     1861 tacttctaga actgtccggt caattccctc agaaaggttg gcaacgcgaa agccgcgggg
     1921 tggatttcgc tgttgtagta tctcaaagga gcgttgaaat ccaggactct ttcttcatcg
     1981 aagtccttaa cagggtcgaa ggttttcgaa ccgagcgtgt aggtccacat gccggacggg
     2041 taagtggtca tgaaactggt gtacatcctg catacgggaa aaaccgaaga gatcctgttg
     2101 aaggctatgg cgacccagcc cttgtcgtag aagggatcct ctgtttcggc acagaagacg
     2161 ccatccttcc taagcgcggc gtagcaattc acatagaact ctttggtaaa gaggtgcccg
     2221 ccctcgccag ctgtgggatc ggtcgaatct acgacaataa catcgaagcg gtctttgaac
     2281 tttctgacgt agtcggcccc gttttcattc actatggtag ctctgggatt atccagttcg
     2341 atcgacattt ccttcagata ttttcttgaa gcctctatca cataaggatc cacttcacac
     2401 agcacagcct cttcgaccga gttgtgc
//
LOCUS       DAOOLY010000328         2421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_54033_length_2421_cov_5.051986, whole genome shotgun sequence.
ACCESSION   DAOOLY010000328 DAOOLY010000000
VERSION     DAOOLY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54033_length_2421_cov_5.051986"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLD15_11740"
     CDS             complement(<1..586)
                     /locus_tag="PLD15_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB64630.1"
                     /translation="MALVYVVDDELNVRRIIKQTMENEGNEVNAFVTAEEALEAVEKR
                     QPDLIFMDLTLPSMDGIQFLKELEDRAYSIPVVVVSAITSPESIRAVFKLGIVDFLTK
                     PFTPQELRQAAELSFKNEESLQKRTREIRRLIEEKEFRKASSLLREFFADFPSSPYPH
                     FLYALLIKSSNPRVAARHLRAALSLDEEFKEASSE"
     gene            complement(710..1099)
                     /locus_tag="PLD15_11745"
     CDS             complement(710..1099)
                     /locus_tag="PLD15_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64631.1"
                     /translation="MSVRNVSTWLFLLGMLYVTLAVNTAFGFLFSAKDLMMGIVILVN
                     GSIYLFGLLERVEDVKKRTSHLLVGSFFSYILSIYQIVVVFSLWLEGFVGGLCLLSVD
                     AINFPLLFSGLLVSFISDYLKKSFTTT"
     gene            complement(1096..>2421)
                     /gene="feoB"
                     /locus_tag="PLD15_11750"
     CDS             complement(1096..>2421)
                     /gene="feoB"
                     /locus_tag="PLD15_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPB64632.1"
                     /translation="RWLAIKSLEHDNGVEKLTGFKAEKDFSSELAETRYRYIKRVLST
                     SVKGKEKHGWDLSEAIDHVITHRVLGIFVFLSVIYIVFQLTFTISGPLSLLIESGLES
                     LGSFLGNSIGPDWLRSLVVDGIIGGVGAILVFVPNIFILFMALGVLEESGYLPRAAFV
                     IDRLMYSMKLSGRSFISMLLGFGCNVSSIMSTRSISEPKERIVTILVSPFISCSARLP
                     VFLLIAGTFFGTKGGVVVFFLYLLSIVVTVLSALIINRLLFKGEPSTMIMELPRYRKP
                     RVSSIMFYTWNKGRHFLEKAGTIILGASVVIWMLSYFPAEGSQSYAAMIGRALEPLFI
                     PLGFSWEMISSLVFGVAAKEVIVSSLTTFYGNYAVMGSAMEVMRVAISPATAFSFLVF
                     VLLYVPCLPTLAVTKNETGSYRYVFFSVIYSLAVAYSVALAVRVIGGLL"
BASE COUNT          694 a          598 c          550 g          579 t
ORIGIN      
        1 actctgatga cgcttcttta aactcttcgt ccagagaaag cgcagcccgc aaatgccttg
       61 cggccactct cgggttagag ctctttatca ggagagcata gaggaagtgt ggataaggcg
      121 aacttggaaa atcggcgaaa aactcacgca gtagagagct ggccttcctg aattcctttt
      181 cctctatcag tcttctaatc tctctcgttc ttttttgaag gctttcttcg tttttgaacg
      241 atagttcggc ggcctgtctc aattcttgtg gagtgaaagg tttggttaga aagtccacta
      301 tgccaagctt aaagacggcc ctgatcgatt cgggagaagt gattgccgaa acaactacta
      361 ctggaattga ataagctcta tcctctaatt ctttaaggaa ctgtattcca tccatcgaag
      421 gtagtgtgag gtccatgaaa atcaaatccg gttgtctctt ttcaacggcc tccagcgctt
      481 cttcggctgt gacaaacgca ttgacttcgt tgccttcgtt ctccatggtc tgtttaatga
      541 tacgtctcac atttagctcg tcgtcgacca cgtaaacgag cgccatgcaa tcacccccaa
      601 acacaaatca tattaccaca tcaacggaca ataatgtcta gtgattcgct ctggccttaa
      661 ttatagccat tacagaattc ggttgtactt ccgaatggtt ctttgacagt taagtcgtgg
      721 tgaagctctt cttgaggtag tcggagatga aggagaccag caaaccagag aagagaagag
      781 gaaaatttat tgcgtcgacc gaaagaaggc ataaaccacc aacaaatccc tctagccata
      841 aactgaagac aacgacgatc tggtaaatcg aaaggatgta agagaagaaa ctgcctacaa
      901 gaagatggga agttctcttc tttacgtcct ccactctttc gaggagtccg aaaagataga
      961 tggaaccgtt cacaaggatc actattccca tcattaaatc ctttgcgctg aagagaaaac
     1021 cgaaggccgt atttaccgcc agagtcacat aaagcattcc caaaaggaac agccatgtag
     1081 agacgtttct gacgctcata acaatcctcc gatcactctg accgcaaggg cgaccgagta
     1141 agccacggcg aggctgtaaa tcacggagaa gaagacatac ctgtagcttc ctgtctcgtt
     1201 ctttgtcacc gccagagtcg gcagacaggg cacatacaac agaacgaaga ccaggaatga
     1261 gaacgccgtc gccggcgaga tcgccactct cataacttcc atcgcagaac ccatcaccgc
     1321 gtagttgccg tagaacgtcg taagcgaaga gactatgacc tcttttgccg ccactccgaa
     1381 gacgagactg gaaatcatct cccaggaaaa gcccagagga atgaacagcg gttccagcgc
     1441 ccgaccgatc atcgccgcgt aactctgcga accttcggcg gggaagtaac tcagcatcca
     1501 gataaccaca gaagcgccca gtattatcgt gccggccttt tcaagaaagt gtctcccctt
     1561 gttccacgtg tagaacatta tagaactcac ccttggcttc ctgtatctgg gcagttccat
     1621 gatcatagtg gaaggctctc ctttaaaaag gagtcgatta attatgaggg ccgatagaac
     1681 agtaactacg atgctcaaaa ggtaaaggaa aaacaccacg acgccgccct tagtcccgaa
     1741 gaaggttccg gcgatgagca ggaatacggg taaccgggca ctacaggaga tgaaaggtga
     1801 gacaagtatc gtcactatcc tctccttggg ctcggagata gatctggtag acattataga
     1861 agatacgtta cagccaaaac cgagaagcat ggatatgaag gatctaccgc tgagtttcat
     1921 tgagtacata agcctgtcta tgacaaacgc tgctctggga agataacccg actcttctag
     1981 tacccccaaa gccatgaaca gtataaagat atttggaacg aagactagta tcgcgccgac
     2041 accgcctatg attccatcca ccaccagaga acgcaaccag tcgggaccta tcgaatttcc
     2101 cagaaagctt ccgagacttt caagaccgct ttctatgagg agcgataagg gaccagagat
     2161 tgtaaaggtc aactgaaaga cgatgtagat gacggataaa aatacgaaga tgcccagaac
     2221 cctgtgagta attacgtgat cgatggcctc ggaaagatcc catccatgtt tttcttttcc
     2281 ctttacggat gtggagagaa ctctttttat atacctgtat ctggtctcgg cgagttcgct
     2341 cgagaaatcc ttctcggctt tgaaacctgt gagtttctct actccgttat cgtgttccag
     2401 agactttatg gccagccacc t
//
LOCUS       DAOOLY010000329         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_54558_length_2405_cov_3.622553, whole genome shotgun sequence.
ACCESSION   DAOOLY010000329 DAOOLY010000000
VERSION     DAOOLY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54558_length_2405_cov_3.622553"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLD15_11755"
     CDS             complement(<1..732)
                     /locus_tag="PLD15_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPB64633.1"
                     /translation="MKIFIGVDVGSVSTNVVALDESENILFKYYIRTNGQPMDVVKFA
                     MSRLREELGEPLVLGVGTTGSGRQLAGVLLGADIVKNEITAHGVAASREVPHVRTIFE
                     IGGQDSKIILIKDGMVVDFAMNTVCAAGTGSFLDHQAERLGIPIENFGDLALKAENEV
                     RIAGRCTVFAESDMIAKQQYGFSKPDIIWGLCRALVRNYINNLGRGKKLEPPFVFQGG
                     VAANKGIKKAFEEAMGCEVIVPANYD"
     gene            complement(754..1845)
                     /locus_tag="PLD15_11760"
     CDS             complement(754..1845)
                     /locus_tag="PLD15_11760"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPB64634.1"
                     /translation="MKYSFPYLGNVLVYKKLMEMLGYEVVVPPRPTQKTIDLGTKNSP
                     EFACFPYKMLMGNYIEVCEQGAGIIVSSGGHGPCRAGFYGELHERTLRHMGFDVELII
                     FDSIKRDLRGDISKFFRLKGKNSLRKVISTGRIILRMLKDLDEFDKKIHTMIPYETVH
                     GSCLKAFEEIRMSYDRCWTMKETEMAYEEAKELLASIKLEEVDERDKIRIGVVGEIFV
                     VIESSINMDIENTLCSLGCEAERSQYIGEWVDHNFFGKGREKEILKMGEDYIEIIIGG
                     HAKQNVGHIVDYSKRGFDGVVHLMPFACLPELVSQSVIPRISEDYGIPVLTLAIDEQS
                     GKANSMTRIEAFVDLIKNSKRKKMAAKTA"
     gene            complement(1842..>2405)
                     /locus_tag="PLD15_11765"
     CDS             complement(1842..>2405)
                     /locus_tag="PLD15_11765"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPB64635.1"
                     /translation="LACRKGAELWRKFRKLRLEGRTIAEAFEGLKNPDGIREPVKIVG
                     PKIGLLGYVYDVYDEFVSMNVTERLRQLGMNVLTFEMLSNRELKRYIKKMKKSLFWTF
                     SDKLLGGAYKMFRERSVDGVIHVTAFGCGPDAFLGKLLELESEDSGIPFMTVRIDEHT
                     GENHLQTRVEAFVDMLKRKTVPVGDAV"
BASE COUNT          451 a          730 c          575 g          649 t
ORIGIN      
        1 atcgtagttg gccggaacta tcacctcgca tcccatcgcc tcttcgaagg ctttcttgat
       61 ccctttgttg gccgcgacgc ccccctggaa gacgaacggg ggctcgagtt tctttcccct
      121 tccgaggttg tttatgtagt ttctaaccag cgccctgcag aggccccaga ttatgtcggg
      181 cttcgagaag ccgtactgtt gtttggcgat catgtcggac tctgcgaaga cggtgcacct
      241 cccggctatc ctgacttcgt tctcggcctt cagcgcaagg tctccgaagt tctcgatcgg
      301 tatgcctagc ctttcggcct ggtggtcgag gaatgagccg gtaccggcgg cgcagacggt
      361 gttcatggcg aaatccacga ccattccgtc ttttatgagt attatcttgg agtcctgacc
      421 gccaatctcg aagatcgttc taacgtgcgg gacctctctg gaggcggcca cgccgtgcgc
      481 ggtgatctcg ttcttgacta tatcggcccc cagcagaacc cccgccagct gtctgccgct
      541 gccggtcgtc cccactccca gcacgagggg ctctcccagc tcttctctca gtctggacat
      601 cgcgaacttg actacgtcca tcggctggcc gttggtcctt atgtagtact tgaagagtat
      661 gttttcactc tcgtcaaggg ccactacgtt cgtacttaca gatcctacat ccactccgat
      721 aaatatcttc atatctccac ccacccgtag atactacgcg gtttttgcgg ccattttctt
      781 ccgcttgctg tttttgatca agtcaacgaa ggcctctatc ctcgtcatgc tgttggcctt
      841 gccgctctgc tcgtctatcg ccagagtcag gaccgggatg ccgtaatcct ctgagatcct
      901 cggtatgacg ctctgagaaa ccagttccgg cagacatgcg aaaggcatca ggtgaacgac
      961 tccgtcgaag ccccgcttgc tgtaatccac tatgtggccg acgttttgct tggcgtggcc
     1021 accgattatt atctctatgt aatcctcgcc catcttgagg atctccttct ccctgccctt
     1081 gccgaagaag ttgtgatcga cccactcgcc tatgtactgc gatctttcgg cttcacagcc
     1141 gagactgcag agtgtgttct ctatgtccat gtttatactc gactctatca cgacaaagat
     1201 ctcgccgacc actcctatac gtattttatc cctttcgtcg acttcttcaa gctttatgga
     1261 agcgagcagc tctttcgcct cttcgtaggc catttccgtc tctttcatag tccagcatct
     1321 gtcgtaggac atcctgatct cctcgaaggc tttgagacag gagccatgaa ccgtctcgta
     1381 cggaatcatg gtgtgtatct tcttgtcgaa ctcgtcgaga tccttaagca tccgcagtat
     1441 gatcctgccg gtcgagatga cctttcgcag agagtttttg cctttcagcc tgaagaactt
     1501 cgatatatcg ccccgcagat cgcgcttgat cgagtcgaag attatcagtt cgacgtcgaa
     1561 gcccatatgt ctgagcgtcc tctcgtggag ttcgccgtag aaaccggccc tgcacggacc
     1621 gtgtccgccg ctggatacta ttataccggc cccctgttcg cagacctcta tgtagttgcc
     1681 catcaacatc ttgtagggga aacaggcgaa ctcgggactg tttttcgttc ccaggtctat
     1741 cgtcttctgc gtcggcctgg gagggactac cacttcgtaa cccagcatct ccatgagctt
     1801 tttgtaaaca agcacgtttc ccaggtaggg gaaggagtac ttcataccgc atcacccacc
     1861 ggaacggtct ttctcttgag catatccacg aaggcctcga ccctcgtctg gaggtggttc
     1921 tcacccgtgt gctcgtctat cctcaccgtc atgaagggaa tccccgaatc ctccgactcc
     1981 agctcgagga gcttacccag gaaggcgtcg gggccgcagc cgaaggccgt gacgtgtatc
     2041 accccgtcga ccgatctctc cctgaacatc ttgtacgcgc ctccaagcaa tttgtcgctg
     2101 aaggtccaga agaggctctt tttcatcttc tttatatatc tcttgagttc tctgttggaa
     2161 agcatttcga aagtcagcac gttcattccg agctgcctca gcctctcggt cacgttcatg
     2221 ctgacgaact cgtcgtaaac gtcgtaaacg tatcccagaa ggcctatctt cggtccgacg
     2281 atcttcacgg gttctcttat cccgtcggga ttcttgaggc cttcgaaggc ttcggcgatc
     2341 gtccttccct ccagtctgag ctttctgaac tttcgccaga gctcggcgcc cttcctgcag
     2401 gccag
//
LOCUS       DAOOLY010000330         2379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_55392_length_2379_cov_5.160499, whole genome shotgun sequence.
ACCESSION   DAOOLY010000330 DAOOLY010000000
VERSION     DAOOLY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55392_length_2379_cov_5.160499"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..883
                     /locus_tag="PLD15_11770"
     CDS             131..883
                     /locus_tag="PLD15_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPB64636.1"
                     /translation="MSEENVVLKIENLKKSFGDKEVLKGVSFEMKKGETKVIIGPSGT
                     GKSTLLACINMLVVPNGGKIWLENEEVTSAKNLNKIRQEIGFVFQDFGLFSHLTALKN
                     VMIGLTKVKKMEKEAARELAMAELKRVGLEKEASLYPAQLSGGQKQRVGIARALAMKP
                     KIILFDEPTSALDPELIGEVLSVMKNLALSGMTMLVVTHEMGFARTVSDEIIFMENGY
                     IVEQSSPEEMFKNPKNQRTKEFLFKLNELYGE"
     gene            889..1560
                     /locus_tag="PLD15_11775"
     CDS             889..1560
                     /locus_tag="PLD15_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB64637.1"
                     /translation="MEKIWNIAVKYWPKLLEGLGVTMEMTLISVVAGFLIGIVLAIAR
                     VYGNKFFYGLSTVIIEIIRGTPLLVQLFILYYGLPPLGVNLSPFIAAIIGFAINSGCY
                     QAEYLRGSIQSISGTQMKAARSLGMSKTEAIMQIIMPQALRRVIPAWTNEFIYLLKYT
                     SLAYIVGAPELMAQAKFIASRNFQFFEVYLVVAVIYLVVVLLFTKIFSILEKKVEIPG
                     LEWVR"
     gene            1653..>2379
                     /locus_tag="PLD15_11780"
     CDS             1653..>2379
                     /locus_tag="PLD15_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPB64638.1"
                     /translation="MLIDGEHLSLKELYDVAYNYEKVEIDATVIELLKQRRLFLEEES
                     KTNTIYGVNTGFGILADKKISPGDLEILQRNLVLSHAAGTGKTLQKELVRAIMLVRTN
                     SLCKGLSGIRPDVVSHLIDMLNEDVIPVVPEKGSVGASGDLSPLAHIAMGMIGLGKCL
                     FRGEVIESQEAIKRAGLKPIVLKAKEGLSLLNGTAFMAGIGGCATYIARQVFEEAITV
                     SAMSVDALMGSTSPFDARIHLARP"
BASE COUNT          667 a          545 c          622 g          545 t
ORIGIN      
        1 acgaaccgat gattatttac ctgacggtag cggttttcta cttcgtcgtt accatcgtta
       61 taaacaagag tcttggaagg gttgagaaga agctggcaat acctggattt gacgtaaagg
      121 agactgttag atgagcgaag aaaacgtcgt tttgaagata gagaatctga agaaatcctt
      181 tggagacaaa gaagtcctga agggtgtttc attcgagatg aaaaagggcg aaaccaaggt
      241 aataataggg cctagcggaa ccgggaaaag cactctcctg gcctgcatca atatgctcgt
      301 tgtacccaac ggcggaaaga tctggctcga aaacgaagaa gtcacctcag cgaaaaacct
      361 gaataagatc cggcaggaga taggcttcgt gtttcaggat ttcggacttt tcagtcatct
      421 gacagccctg aagaacgtca tgatcggtct caccaaggtg aagaaaatgg agaaagaggc
      481 tgccagagag ctcgcgatgg cagaattgaa gcgagtcggt ctggagaagg aagcctcgct
      541 ctacccggca caactctccg gagggcagaa acagcgggtg ggcatagcca gagccctcgc
      601 gatgaagccg aaaataatcc tcttcgacga accgacatcg gccctggacc cggaattgat
      661 aggagaggtt ctgtcggtca tgaagaacct tgccctcagc ggtatgacca tgctggtggt
      721 gacacacgag atgggattcg ccagaaccgt ctccgacgag atcattttca tggagaatgg
      781 ttacatagtt gaacagagtt ctccggaaga gatgttcaaa aaccccaaga atcaaagaac
      841 caaggagttc cttttcaaac tcaacgaact ctacggggag tgaatcgcgt ggagaaaata
      901 tggaacatcg ctgtaaaata ctggcccaaa ttactggagg gtctcggagt taccatggaa
      961 atgaccctga tttctgtggt ggccggtttc ttgatcggca tcgtcctggc catcgccagg
     1021 gtttacggaa acaagttctt ctacggctta tcgacagtaa taatcgagat aataagggga
     1081 acacctttac tcgtgcagct tttcatactc tattacgggc ttcctccgct gggggtcaac
     1141 ctctccccct tcatagccgc gataataggc tttgcgatca acagcggctg ttaccaggcc
     1201 gaatatctca gaggatcgat acaatccatt tcgggaacac agatgaaggc tgccagatct
     1261 cttggaatgt ccaaaaccga ggcaataatg cagataataa tgccgcaggc tctcagaaga
     1321 gtcatcccgg catggactaa cgaattcatc tatcttctca agtacacttc tctggcctat
     1381 atcgtcggcg ctcccgagct catggcgcag gccaagttca tagcgtcgcg caacttccag
     1441 tttttcgaag tctatctggt ggttgcggtt atctatctag tcgtggtgct tttattcacc
     1501 aagatcttca gcatactcga aaagaaagta gaaatccccg ggcttgaatg ggttagataa
     1561 cctccagagt ttataacgaa ccaaacggag ctttgaacaa cacctccgct cgaggttata
     1621 atcggttata aagaagaagc ggaggtgttg ttttgttaat cgatggagag catctgtccc
     1681 tgaaggaact ctacgatgtt gcctacaact atgaaaaggt agagattgac gctacggtaa
     1741 ttgaacttct gaaacagaga aggctgtttc tcgaagaaga gagtaaaaca aacaccatat
     1801 acggtgtcaa cacgggtttt ggaatcctgg ccgataaaaa aatatctccg ggcgacctcg
     1861 agattcttca acggaacctg gttctctctc acgcggccgg aacgggaaaa acgctgcaaa
     1921 aagagcttgt gagggctata atgctggtca ggaccaattc actatgtaag gggctgtcgg
     1981 ggataaggcc cgacgttgtc agtcatctga tcgatatgct caacgaagat gtgatcccgg
     2041 tcgttccgga aaaaggctca gtcggtgcca gtggcgatct gtcaccgctt gcccatatcg
     2101 ccatgggaat gataggtctg ggaaagtgtc tcttcagagg cgaggttatc gagtctcagg
     2161 aagccatcaa gagagcgggg ctcaaaccga tcgtgttaaa agcaaaagaa gggttgagcc
     2221 ttcttaatgg aactgccttc atggctggaa taggaggctg cgcaacctac atagcccgcc
     2281 aggtattcga agaggccata accgtctcgg cgatgtccgt cgatgcgctc atgggtagta
     2341 cttcaccctt cgatgcgagg atccaccttg ccagaccac
//
LOCUS       DAOOLY010000331         2371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_55666_length_2371_cov_4.561313, whole genome shotgun sequence.
ACCESSION   DAOOLY010000331 DAOOLY010000000
VERSION     DAOOLY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55666_length_2371_cov_4.561313"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLD15_11785"
     CDS             <1..276
                     /locus_tag="PLD15_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPB64639.1"
                     /translation="QLIITERLHGAILAISMGIPFVWRKNSKLDSFVKSLDGRCDLNF
                     EDGCEGLTIAINNSLKYSVDLKGEYLSRLNETVETSKGIMRKIIDRG"
     gene            281..1381
                     /locus_tag="PLD15_11790"
     CDS             281..1381
                     /locus_tag="PLD15_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HPB64640.1"
                     /translation="MWRYFLPTRIFSGSGVLKSNQTVFKENGTKALVVTGRSSARLSG
                     ALDDAIEILKNLDVPYALFDEVEENPSFATVEKGGRIMEENDCDFVVAIGGGSPLDAA
                     KAISALNKNREITCDDLYRGDSLVAFPIIAIPTTSGTGSEVTPYSILTDGNGIKKGFG
                     MPSAFPIVSFLDAGYTLTMSREVTLATALDALSHSLEGEIVNNGYNPLVKMLSVESTG
                     IIYRVLPRLIDRERDFALREKMQYAAMLAGMVIAHTGTTAVHAAGYPLSSFKGVKHGI
                     ANAVTMIKVFERIAQADPERIARAIEPFKDLGELEDFLDSFRVQEMAPILTEEEVQRW
                     SELSSKASHNKKTPGDLDRDFYVELYRGLGKR"
     gene            1378..>2371
                     /locus_tag="PLD15_11795"
     CDS             1378..>2371
                     /locus_tag="PLD15_11795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPB64641.1"
                     /translation="MNSAVLKRNIKRRISAGHPWIYDNEIERCESPLDGSLVDVFTFS
                     GQFVGRGYYNSNSIIRIRLLTRKNVPIDREFFKERFSRVFRAREGLLRNSNAVRVIFG
                     EADGVPGLIVDRFSDYLVVQFNTLGINTFRDEIVESLVKLFNPPGIYEKSEGLALSKE
                     GLERREEWIHGKGPELIPFVLNGIRFLADTKGQKTGFFLDQRENALRLSPMSDGGKVI
                     DLFSYTGNFALHCLAGGAREATLVDISQRALDVARESASLNGWKDRCTFITANAFDYL
                     RESDQSSADIIVIDPPAMAKSSSSKANALRGYKELNLRAIKSLRSGRLLATSSCT"
BASE COUNT          631 a          581 c          636 g          523 t
ORIGIN      
        1 caacttataa tcaccgagag actccacgga gcgatactgg caatctccat gggaataccc
       61 ttcgtgtggc gtaaaaacag caaactcgac agtttcgtca agtcgctgga cggtcgatgc
      121 gacctcaact ttgaggatgg ttgcgagggt ttgacgatag cgatcaacaa ctctttgaag
      181 tactcggtcg atcttaaggg agaatatctc tcccgcctga acgaaactgt agagacaagc
      241 aaaggaatta tgagaaaaat tatagataga gggtgatgag atgtggagat actttctacc
      301 aacgagaatc ttctcgggaa gcggtgtcct gaaaagtaac cagacggtct tcaaagagaa
      361 tggaacgaaa gccctcgtag ttaccggtag atcatcggcc cggctcagcg gcgcccttga
      421 cgatgcaatc gagattctca agaacctcga tgtgccttat gcgctcttcg acgaggtcga
      481 ggagaaccca tcctttgcga cggttgagaa gggtggaagg atcatggagg agaacgactg
      541 tgatttcgtt gtcgccatcg gtggaggcag tccgctcgat gcggccaagg ctatctcggc
      601 tctcaacaaa aacagagaaa taacctgcga cgacctttat cgaggcgatt cgctggtggc
      661 ctttccgata atagcgattc ccacaacctc gggaaccgga agcgaggtga cgccctactc
      721 gatcctgacc gacggtaacg gtataaagaa agggttcgga atgccctcgg cttttccgat
      781 cgtttccttt ctggatgcag gatacaccct caccatgtcc cgagaggtga ctctggccac
      841 agctctggat gcgttatccc attcactcga gggagagata gtgaacaacg gatacaaccc
      901 tctggtgaaa atgctctccg tagaatccac cggaataatc tatcgtgttc ttccccggct
      961 tatcgacaga gaaagagatt tcgcccttag agagaaaatg cagtacgccg ccatgctcgc
     1021 cggcatggtg atagcccata caggcaccac ggccgttcat gcggccggct atccgctctc
     1081 ttcgttcaaa ggcgtcaagc atggaatcgc aaacgccgtg actatgataa aggtcttcga
     1141 aagaatagcg caggccgatc ccgaaaggat agccagagcc atcgagccat tcaaggatct
     1201 cggggagctc gaagacttcc tcgatagctt cagggtccag gaaatggcgc ctattctcac
     1261 cgaggaggag gtacagagat ggtcggaact gtcgtcgaag gcctctcaca ataagaaaac
     1321 tcctggagat ctcgatagag acttttacgt agaactctac agggggctgg ggaaacgttg
     1381 aattccgccg tactgaagag gaacatcaaa aggcgcatct cggcagggca cccctggatc
     1441 tacgataatg aaatcgaaag gtgcgaatca ccgctcgatg gaagcctggt cgatgttttc
     1501 actttttccg gccagttcgt gggacgcgga tactacaatt cgaactcgat catacgcata
     1561 aggctcctga cgcggaagaa tgtgccaata gacagagaat tcttcaaaga aaggttttcc
     1621 agggtctttc gcgccagaga aggccttcta cgtaacagca acgcggtacg ggtcatattc
     1681 ggagaggcag acggagtacc gggtttgatt gttgacaggt tttccgacta tctggtcgtt
     1741 cagttcaaca cccttggaat aaacactttc agggacgaaa tagtcgaatc gctcgtgaaa
     1801 ctcttcaacc ctccggggat ctatgaaaag agcgagggtc tggcgctgag caaagagggt
     1861 ttggagagaa gagaggaatg gatacacggc aaagggcctg agttgatccc tttcgtgttg
     1921 aacggcatca gatttcttgc agacacgaag ggccagaaga ccggtttttt cctcgatcaa
     1981 cgtgaaaatg ccctaaggct ctcgcccatg agtgatggtg gaaaggtgat cgatctcttc
     2041 tcgtacacgg gaaattttgc gcttcactgt ctcgccggag gcgccagaga ggcaacgtta
     2101 gtcgatatat cccagagagc cctggatgta gccagggaaa gtgcttcact caacggatgg
     2161 aaagacaggt gcactttcat caccgccaac gccttcgatt atttacgtga aagcgatcag
     2221 tcgtcggccg atataatcgt tatcgatcct cccgcaatgg ctaaatcttc ctcatctaag
     2281 gcgaatgccc tgagggggta taaagaatta aacctcaggg caatcaagag cctcaggagc
     2341 ggtcgtcttc ttgccacttc gtcctgtaca c
//
LOCUS       DAOOLY010000332         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_55708_length_2370_cov_4.404320, whole genome shotgun sequence.
ACCESSION   DAOOLY010000332 DAOOLY010000000
VERSION     DAOOLY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55708_length_2370_cov_4.404320"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLD15_11800"
     CDS             <1..988
                     /locus_tag="PLD15_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB64642.1"
                     /translation="SNEWSVGELFQGAIDIIKKGIDVSPLRELLEELISENTVRNSGI
                     DFGLVTFDLTDLRPEMLFIEDIPEGKLIDYIMASADFPGFQTVRVDGKAFIDGGIYSN
                     QPVEMALQRGFRNIVLVDIGRVALRDRIARFQAFVQGAEVTHIVPRIMYGSTLDFDPE
                     KIQLQIEEGYLDTLAAFGLVTGEYTYIFEDRDVLEVMFDSLPVQKQLEAIEFIGDPAR
                     WPSPESFYEDFAGSLEKIYKLPMPTLVLLDNLARQLKIGNLMLYSTGEILKEIKTAYE
                     RDGIELPEESLIPYDRAVDFVLYLYENAPLPESPENFSFFKSSFDTLSDVKE"
     gene            994..1575
                     /locus_tag="PLD15_11805"
     CDS             994..1575
                     /locus_tag="PLD15_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64643.1"
                     /translation="MKWILSVIVILLIFSGCVPMSLLMGPEIQPKGTVKHAVGMDLLV
                     GFNEAIVVPWTFQYLARVGLTDYVEASFRAALPSILISSPSITLEGGAKFGVPGFSNV
                     ALLASGGFFFLSKSGNNETLFEPYMRIAPIVGFKIMDWTIAAKLQLFVASEVGFIPGI
                     SVNNGRFYFDIDFPLVGGTTIPDGGLIGIGYAF"
     gene            complement(1652..>2370)
                     /locus_tag="PLD15_11810"
     CDS             complement(1652..>2370)
                     /locus_tag="PLD15_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB64644.1"
                     /translation="PHISTGLKSHKFGIPIEKIDRAADRIDGLHVHIGSQITEVAPFL
                     DAYSKVLDCAKKYGFKYINLGGGWGIDYSGDSLDLEALKSGITALFSDYDGKLISELG
                     RFIIGPAGYLVAGVVSVKPSDKVFVVTDAGMNTLIRPVLYDAHHRIHILNGTGKTVTA
                     DVVGPLCESGDILARDRRLEIPIPGSAIVFENAGAYGFSMASNYNGMPLPAEVLVDGK
                     EVRLIRKRQNIEELFINVKI"
BASE COUNT          592 a          572 c          580 g          626 t
ORIGIN      
        1 ttcgaatgaa tggagtgtcg gtgagctttt ccagggagct atcgacataa taaagaaggg
       61 catagacgtt tcgcctctga gagaattgct tgaggaactg atttcggaaa atactgtcag
      121 aaactccggt atagactttg gacttgttac tttcgacctg acagatctac gcccggaaat
      181 gcttttcata gaagacattc ctgagggaaa gctaatagac tacataatgg ccagcgcaga
      241 tttcccgggc tttcaaactg ttcgtgtcga tgggaaggct ttcatagatg gggggatcta
      301 ttcgaatcag cctgtggaga tggctctcca gagaggattc agaaacatcg tactcgtcga
      361 tatcggacgg gttgcactga gggataggat cgccagattt caggccttcg tacaaggcgc
      421 agaagtcaca cacatagttc cgcgcatcat gtatggcagc actctcgact tcgatcccga
      481 aaaaatacaa ctgcagatcg aagaaggtta tctagatacg ctggccgctt tcggcctggt
      541 cacaggtgaa tatacctaca tcttcgaaga tcgagatgtt ctggaggtaa tgttcgactc
      601 gcttccggtg caaaaacaac tcgaggcgat cgagttcata ggcgatcctg cgagatggcc
      661 ttcccccgaa agtttttacg aggatttcgc cgggtcgctt gaaaagatct acaaactgcc
      721 gatgccaact ctggttttac tggacaacct cgcccgtcag ctgaaaatcg gcaatctgat
      781 gctatacagt accggcgaga tacttaagga gattaaaacc gcctacgaaa gagacgggat
      841 cgaactaccc gaagaatcat tgattccgta cgacagggcc gtcgattttg tactctatct
      901 ttacgagaac gccccgttgc cggaatcccc cgagaacttt tctttcttca aatcctcttt
      961 cgacactttg agtgacgtaa aggagtgatc gttatgaaat ggatattatc ggttatagta
     1021 atacttttga ttttttcggg ttgtgttccg atgtctctgc ttatggggcc ggaaatccag
     1081 ccgaaaggga ccgtgaagca cgccgttgga atggacttgc tcgtcggctt taacgaagcc
     1141 atcgtcgtcc cgtggacttt tcagtacctg gcaagagtcg gtttgaccga ttacgtagaa
     1201 gcctctttca gagctgcttt acccagtatc ctcataagct ctccttcgat aactctcgaa
     1261 ggaggtgcca agttcggagt tccgggattc tcaaacgtgg ctctcctcgc gagcggaggc
     1321 ttcttctttc tatctaaatc cggcaacaac gagactctgt tcgaaccata tatgcggata
     1381 gcgccaatcg ttggattcaa gattatggac tggacgatag cggcaaaact ccagcttttc
     1441 gtggcaagcg aagtcggctt tattccaggc atctcggtga acaacggccg gttttatttc
     1501 gatatcgatt ttccactggt tggaggtacg acaattcccg atggaggatt gataggtatc
     1561 ggttatgcct tctaggacaa tatagaacag tcggggtcgg aagaggtccg actccgctgt
     1621 ttttgcaaac actcccgtac aactgaagag atcatatctt tacgtttata aaaagctctt
     1681 ctatgttctg gcgtttcctt atgagcctta cttctttacc atctacgaga acttctgccg
     1741 gaagcggcat accgttgtaa ttgctggcca tcgaaaagcc atatgcaccg gcgttttcga
     1801 agactatggc gctgcccggt ataggtatct ccagcctcct gtcgcgggcc agtatatctc
     1861 cgctctcgca tagcggaccg acaacatcag cggttacagt cttcccggtt ccattcagaa
     1921 tgtgtatcct gtgatgcgca tcgtaaagca caggtctgat gagagtgttc atacccgcat
     1981 cggtgactac aaagacctta tctgagggtt tcacgctcac cacaccggca acgaggtagc
     2041 cggccggccc tattataaaa cgtcccagtt cgctgatcaa cttaccgtcg taatctgaaa
     2101 aaagggcggt tattcccgac ttcagagctt caaggtcgag tgagtcacct gagtaatcta
     2161 ttccccagcc tccaccgagg ttaatgtatt tgaaaccgta cttcttcgca cagtcgagga
     2221 ctttcgagta agcatccaaa aaaggtgcca cttcggtgat ctgagaaccg atatgcacat
     2281 gcaaaccgtc tatcctgtcg gcagctctgt cgattttctc gatgggaatg ccgaatttgt
     2341 gcgatttcag accggtagat atatgtggat
//
LOCUS       DAOOLY010000333         2344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_56550_length_2344_cov_4.422018, whole genome shotgun sequence.
ACCESSION   DAOOLY010000333 DAOOLY010000000
VERSION     DAOOLY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56550_length_2344_cov_4.422018"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1171
                     /locus_tag="PLD15_11815"
     CDS             125..1171
                     /locus_tag="PLD15_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB64645.1"
                     /translation="MTVKKRIVFTAVAIICLIIVLYLSQEFMSSYYSRIITLIGIYGI
                     MAVSLTLVNGISGVFSLGHAGFIAVGAYTSALLTLSPQQKEITFLIEKLIWPFNSIQL
                     PFFWATLIGGVVAAIFALAIGWPSLRLTGDYFAIATLGFAEIIRIFALNLNSLTNGAL
                     GLKALPDHSNIWASWGWLLVTVACVTSLSYSSFGRALRAIREDRVAAQAMGINVFKHQ
                     LMAFVVGGFFAGISGSLYAHWLSTIDPRTNTIGIMLTFNVLIMIVLGGLGSITGAIIG
                     GALFAFLSEWLRFLEGPMNILGFKIMGITGLRMLVFSSLFVVIMIFWPRGIMGRREFS
                     WDGLASLFKRRDRK"
     gene            1168..1962
                     /locus_tag="PLD15_11820"
     CDS             1168..1962
                     /locus_tag="PLD15_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64646.1"
                     /translation="MRVLLLEGITMQFGGLTAVGDVSLEINEGELFGLIGPNGAGKTT
                     VFNMITGHYKPTRGKIYFREKEITGLIPDRITRMGIARTFQNIRLFQDLTVLENVMVA
                     QHHSISSGGQAARWFIKSVTRIGYSVKEREMKEKALNLLKVLGIEGLANDKSGSLSYG
                     AQRLLEIARAMATGASLLLLDEPAAGMNASETAQLVHTIRKIRNDFNLTVLLIEHDMK
                     LVMGLCERIMVLDYGRTISEGDPAYVQKDRKVIEAYLGKEWVTIGR"
     gene            1952..>2344
                     /locus_tag="PLD15_11825"
     CDS             1952..>2344
                     /locus_tag="PLD15_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64647.1"
                     /translation="MEDEILKIENLQVSYGSIKAVKGISISVRKGSIVTIIGSNGAGK
                     STTLGAISGLTRPSGGKITFRDRDITRLGAARTSQLGISLVPEGRRIFPNLTVRENMM
                     MGAYNRKNREEVQENFQTVFTLFPRLKER"
BASE COUNT          583 a          544 c          651 g          566 t
ORIGIN      
        1 gttggcctct tccccggagc ggccgggtac agagacgcat tcgcattttt catactcata
       61 ataatcctct cggtcaaacc ttccggtctc ctcggaaaac cagagctagt aaaggtgtga
      121 tgagatgacc gtaaagaaga ggattgtttt cactgccgta gcaattatct gtctgattat
      181 tgttctgtat ctctctcagg agttcatgag cagctattat tcgaggataa tcacgctcat
      241 aggtatttac gggataatgg cagtcagcct gactctggtt aacggtatct ccggtgtttt
      301 ctcgctcggg catgccgggt ttatagcggt cggagcctac acgtcggctc tgctgacttt
      361 atctcctcaa cagaaagaga taacctttct catagagaaa ctgatctggc ctttcaactc
      421 gattcaactt cccttcttct gggcaaccct tatcggtgga gtagtcgcgg cgatctttgc
      481 gcttgccata ggctggcctt cgcttcgcct gaccggcgat tacttcgcta tcgcgactct
      541 gggtttcgcc gaaatcataa ggattttcgc actgaacttg aattcgctca ccaacggtgc
      601 tcttggattg aaagcccttc ccgaccactc caacatctgg gcgtcttggg gatggctgct
      661 ggtaacggtg gcctgcgtga caagcctatc atacagctct ttcggcaggg ctttgagggc
      721 gatacgcgag gacagggtgg cggcccaggc tatgggtata aacgtcttca aacaccagct
      781 catggccttc gttgtgggcg gtttctttgc gggcatatcg ggctctctct acgctcactg
      841 gttgagtacg atagatccca gaacgaacac gataggtatt atgcttacgt tcaacgtgtt
      901 gatcatgata gttctcggcg gcctggggag tatcaccgga gcaatcatag gcggtgccct
      961 tttcgctttt ctgagcgagt ggctgaggtt tctggaaggt ccgatgaaca ttctcggctt
     1021 caagataatg ggaataaccg gtcttagaat gctcgttttt tccagtcttt tcgtcgttat
     1081 aatgattttc tggcccaggg ggatcatggg aagacgtgag ttctcctggg acggtctcgc
     1141 ttccctcttc aaaaggaggg atagaaaatg agagttctgt tgctagaggg tatcaccatg
     1201 cagttcggcg gtttaacggc cgtaggcgac gtgagcctcg agatcaacga gggcgaattg
     1261 ttcggcctga taggaccgaa cggggccggc aagacgacgg ttttcaacat gataacgggc
     1321 cattacaaac cgaccagggg gaagatatat ttcagggaaa aggagatcac cgggctcata
     1381 cccgacagaa taaccaggat gggtatagca aggacttttc aaaatatacg cctctttcag
     1441 gatctgacgg ttcttgagaa cgttatggtc gcccagcatc acagtatatc cagcggcgga
     1501 caggctgcaa gatggttcat aaaaagcgtc accaggatcg gctactcagt caaagagagg
     1561 gaaatgaagg aaaaggccct gaatcttttg aaggtactgg gtattgaagg actggcgaac
     1621 gataagtcgg gctcgctttc ctacggcgca cagagacttc tagaaatcgc cagggccatg
     1681 gctaccgggg cgtcacttct ccttctggac gagccagcgg ccgggatgaa cgcctccgag
     1741 actgcccagc tggtccacac gatacgaaaa ataaggaacg atttcaacct gaccgttctt
     1801 ctcatagagc acgacatgaa actggtgatg gggctttgtg aaagaataat ggtgctggat
     1861 tacggaagaa ctataagcga gggtgacccg gcgtacgttc aaaaggatag aaaggtcata
     1921 gaggcttacc tggggaagga gtgggtcaca attggaagat gaaatcctga aaatagaaaa
     1981 tctccaggtc agttacggct cgataaaggc cgtgaagggg atctccatct cggtgcggaa
     2041 aggtagtatc gtgaccatca taggttcgaa cggcgccgga aagagtacaa ctctcggagc
     2101 tataagcgga ttgaccagac cttctggagg gaagatcact ttcagggaca gagacataac
     2161 ccgtctgggc gcggccagaa ccagtcagct aggtatatcg ctcgtaccgg aaggccgaag
     2221 aatcttcccg aatttgactg tgcgggagaa catgatgatg ggtgcctaca acagaaagaa
     2281 cagggaagag gttcaggaga actttcagac ggtctttaca ctctttccca gattgaagga
     2341 gagg
//
LOCUS       DAOOLY010000334         2342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_56638_length_2342_cov_4.037604, whole genome shotgun sequence.
ACCESSION   DAOOLY010000334 DAOOLY010000000
VERSION     DAOOLY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56638_length_2342_cov_4.037604"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..828
                     /locus_tag="PLD15_11830"
     CDS             88..828
                     /locus_tag="PLD15_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPB64648.1"
                     /translation="MRFLDFDLQDRDLDSAASELLGGTKSDKKYFVLTLNALIVYEYL
                     HNRDYHEALKRVNYVVPDGSGVLKAVKLLNKKKVSRVPGIDLMLTLCKLASIGSERIY
                     LLGSQIDTVTKTIRKLKERYPGLNIVGSHNGFFDESESDTIVSEINNSRADILFVGMG
                     VPKQELWITKNLDRLNVGVLMGVGGSFDVISGNLNRAPVWMQKIGLEWLYRTVQEPRK
                     RLRLIPKLAKFELHILKSYFCLRRGGSG"
     gene            825..1976
                     /locus_tag="PLD15_11835"
     CDS             825..1976
                     /locus_tag="PLD15_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB64649.1"
                     /translation="MIVTNVMAFTNDFTNPLVDDALVRFENGEITYVGPRREYSIQED
                     EEVVDGERGLLLPAFCNAHLSLYSSISSYPLKLIKGYESGAGYFSNLLKSGYSNTNEK
                     IVKVSAEVAVLKALKSGTVSFSGPIFDCAEVDTEFYRELARRYGMNIAVGPVVVKETI
                     ESVTERWQEVPRDSNFCPLIYVSELATYDEEDLTKLRNLYSKGINLNLFIFDMKQEND
                     LCLSRWGESLIERLLKNGILVPECGIIYGGNLSETDMDIISNRRIFVTKSIRSEMYAG
                     TFQPNIADLLGRGMHVSIGTGFMGTELIEEAKEIVLSERYHKHYDNVVIDYEIRKTLF
                     DNNYRLAQRFFGKRTGVLKEGYSADLLLAKPVLDFERLDPDLPTVLQLI"
     gene            complement(2092..>2342)
                     /locus_tag="PLD15_11840"
     CDS             complement(2092..>2342)
                     /locus_tag="PLD15_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB64650.1"
                     /translation="TASQAVEYFIRSRGIRFARHIPRIPLQVAINLGREFGTLYNLLE
                     STEDELKDVDGIGEKRASAIRHAIDIYKKSGQVTIPGF"
BASE COUNT          656 a          514 c          579 g          593 t
ORIGIN      
        1 gggttgagag ccccgtgcgg gttcgattcc cgccctcggc accagaggaa ggcttagcct
       61 tcctcatttt ttgtggaggt gttctctttg agatttctgg acttcgatct acaggacaga
      121 gatctggatt cagcggcatc tgagttactc ggtggaacga agtccgacaa aaaatacttc
      181 gtcctcacac tgaacgcatt gatagtttac gagtatctcc ataaccgcga ttaccatgag
      241 gcgcttaaga gagtgaacta cgtagttcca gatggaagcg gggtcctgaa agccgtgaaa
      301 ctactcaata agaagaaggt tagcagagtt cccggcatag acttgatgct caccctctgt
      361 aaactggctt ccattggcag tgaaaggata taccttctag gctctcagat cgatacagta
      421 acaaaaacca tcagaaaatt aaaagaaaga tatcctggac ttaatatagt gggctctcac
      481 aacggtttct ttgatgaaag tgaaagtgat acaatcgttt cagaaataaa caattcacgt
      541 gcggatatac tgttcgttgg aatgggtgta cctaaacagg agttgtggat cacgaaaaat
      601 ctggacagat tgaatgtcgg ggttctaatg ggtgtcggtg gttccttcga tgtcatttcg
      661 ggcaatttga atagagctcc tgtgtggatg caaaagatag gtcttgagtg gctttataga
      721 accgttcaag aaccaagaaa acgtttgagg ttgataccca aactagctaa atttgaactt
      781 catattctta agtcgtattt ttgtttgaga agaggtggtt ccggatgatc gtaaccaatg
      841 tgatggcgtt cacaaatgat ttcacgaacc cccttgtgga tgacgcgctg gtcagattcg
      901 agaatggaga aattacctac gtcggtccta gaagggagta cagtattcag gaagacgaag
      961 aagtggttga tggagagagg ggcctcttac ttccggcttt ctgcaacgct cacctgagtc
     1021 tttattcaag tatttcgtcg tatcctctca agcttataaa gggttatgag agcggcgctg
     1081 gatatttcag caatctgctg aaatccggat actccaacac caacgagaag atagtcaagg
     1141 tgtcggctga agtagctgta ttgaaggctt tgaagagcgg tacggtttcg ttcagcggac
     1201 ccattttcga ttgtgccgaa gtggatacgg agttttacag ggaactcgca cgcagatatg
     1261 gaatgaacat tgcagtcggc cccgtggtgg tcaaggaaac gatcgaatcc gttaccgaac
     1321 ggtggcagga agtaccccgg gacagcaatt tctgtcctct tatatatgtt agcgaactgg
     1381 cgacctacga cgaagaagac ctgacaaagc tgcgcaacct ctattccaag ggtataaact
     1441 tgaacctctt cattttcgac atgaagcaag agaacgattt gtgtctctct agatggggcg
     1501 aaagccttat agagaggctg ctgaagaacg gtattctcgt tcccgaatgt ggcattattt
     1561 acggtggaaa tctgagcgag accgacatgg atatcatctc gaacagaagg atattcgtca
     1621 ccaaatctat acgctccgag atgtacgctg gaaccttcca gcctaacatc gccgaccttc
     1681 ttgggagagg gatgcatgtc tcgataggaa caggcttcat gggaacagaa ctaatcgagg
     1741 aggccaagga aatagtgctt tcggaaagat atcacaagca ctatgacaac gtggtaatcg
     1801 actatgagat acgaaaaacg cttttcgaca acaattacag gctggctcag cgcttcttcg
     1861 gaaagcgcac aggggtactc aaagagggct attcggccga ccttctactg gccaagccgg
     1921 tactggattt cgaaagactc gaccccgatc tgcccaccgt tctacagctg atctaaaaga
     1981 tctcctgtga atcgaattat gaaaaagtct ggaacactgg agaattaata ctcgacaaag
     2041 gtttccccca aaggatatcc aggaaggaac tcaaagacct ctccagagat atcaaaaacc
     2101 tggaattgta acctgtccgg actttttata tatatcgatc gcgtgcctta ttgccgaggc
     2161 gcgcttttct cctataccat cgacatcctt taactcgtct tccgtgctct caaggagatt
     2221 atacagcgtt ccaaactccc ttcccagatt tatggccact tgaagcggga ttcgcggaat
     2281 atgccttgcg aaacggatgc ccctggaacg tatgaagtac tccaccgcct gagatgccgt
     2341 ag
//
LOCUS       DAOOLY010000335         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_57453_length_2319_cov_3.943905, whole genome shotgun sequence.
ACCESSION   DAOOLY010000335 DAOOLY010000000
VERSION     DAOOLY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57453_length_2319_cov_3.943905"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLD15_11845"
     CDS             complement(<1..365)
                     /locus_tag="PLD15_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB64651.1"
                     /translation="MKKSVLAILFLMILSLALSNVGLVLSGGGAKGGYEIGAWKALID
                     MDITIGGVYGTSVGSLNAAAVAQGDFGKALEVWRDISESKVMKPTQGQKTLIDAYGGS
                     NEWSVGELFQGAIDIIKKG"
     gene            complement(447..1355)
                     /locus_tag="PLD15_11850"
     CDS             complement(447..1355)
                     /locus_tag="PLD15_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPB64652.1"
                     /translation="MNPIVGKVLTFFILIGVGFTIKKVRLVDDSFTKGLSTFVLYVTL
                     PALVIDSMQFQFSFEMLSSSLILFLSGGFLYTFLWIVGLVVVRLLKLEGKARAVVLYA
                     SLLGNVAYMGYPVVEMLLGKEGLFYTGVFNIWFNVLTWTAGVRIIAGGEFGKRKKCLL
                     NPGIISLLIGLFLFVFSIPLPSFLDESLSMLGECTVPLAMVIAGITLAGAKMKSVLGD
                     QRVIVYSLFKLLAVPLTVFFFLQLVELPGVVEKILIIMSAMPAAANTSIFARIYDSDY
                     DLASRLIASSTFFSLVSIPVIISLFD"
     gene            1623..>2319
                     /locus_tag="PLD15_11855"
     CDS             1623..>2319
                     /locus_tag="PLD15_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HPB64653.1"
                     /translation="MRKVLLVLVAVLVSAAFLVAADFHIGVVTGTVSQSEDDLRGAEA
                     LIKKYGDAASGGMIVHLTYPDNFMSEQETVIAQITGLADDPLMKAIIVNQAIPGTVES
                     FRRVREVRPDILLLAGLPHEDPPMLAAAADLSVNADSIARGYLIIYTAKQLGAEKFMH
                     ISFPRHMSYELLSKRRDIMRAACEELGLEFIDMGSPDPVSDVGVAGAQQFILEKVPAW
                     LQEFGNKTAFFCTN"
BASE COUNT          655 a          588 c          519 g          557 t
ORIGIN      
        1 atgccctttt ttattatgtc gatagctccc tggaaaagct caccgacact ccattcattc
       61 gaaccgccgt aagcatctat caacgttttt tgtccctgtg ttggcttcat cactttgctc
      121 tcggagatat ctctccatac ctccagagcc tttccaaaat caccctgggc aactgctgca
      181 gcgttcagag atccgacaga agtaccatag actccaccta tagttatgtc catatcaatc
      241 aaggccttcc aggccccgat ttcgtaacca cctttggcac cgccaccgga aagaaccagt
      301 ccgacattcg acaaagccag agacaaaatc atcaagaaca atatggctag aacggacttt
      361 ttcattgatc tcacctctct ttcggttagc taaatgatac catcacggct tatacctgaa
      421 aaagatggag cgaatatctc caccgttcag tcaaataacg agattattac gggaatactc
      481 accagcgaga aaaaggtcga agacgcgatc aacctcgatg ctagatcgta atcggaatcg
      541 tatattctgg cgaatatgga agtattggct gccgccggca tggcgctcat aattataagg
      601 attttctcta cgacaccggg cagttctacc aattgcagga agaaaaagac agtcagagga
      661 actgcaagaa gtttgaagag gctgtatact ataactcttt ggtctcccag cacactcttc
      721 attttggcgc cggccagagt aatccccgcg ataaccatgg ccagggggac ggtgcactct
      781 ccgagcatac tcaacgactc atccagaaaa ctcggtaagg gtatcgaaaa aacgaacagg
      841 aacaacccaa ttagaagaga gattatcccc ggattgagaa gacacttttt ccttttgccg
      901 aactctcctc cggcgatgat tctcacaccg gccgtccatg taagaacatt gaaccagatg
      961 ttgaacacac ctgtgtagaa gagaccttct ttacccagaa gcatctccac tacggggtac
     1021 cccatataag ccacgtttcc cagtagcgaa gcgtaaagaa caactgccct ggctttccct
     1081 tcgagcttta gtaatctgac tacgacgagt ccgactatcc atagaaaagt gtagagaaat
     1141 cccccgctca agaacaagat caaagagctg gaaagcattt cgaatgaaaa ctggaattgc
     1201 atggagtcaa ttacgagcgc tggaagagtc acataaagaa cgaaagtaga cagtcctttc
     1261 gtgaaggaat cgtcaacgag ccttaccttt tttattgtga agccgacccc gatcaaaatg
     1321 aagaaagtca ataccttacc gactatcgga ttcatcagga taattataag ccagtatatt
     1381 caagaaagac cgtgatgccg aaagaataga gttattgcgg gaggctttgt acccaaaagt
     1441 gatcttaatc aggatttctc aaaggccatt cgattgtgat aaacttgtaa atgagatttg
     1501 atcctttcct tttttctgtc cgggatcgcc ggtttctatt cggtcgtgag agtggaaatg
     1561 atattattca ggatgccatt ttggcagcct atacacctta aacagaaaaa gggggttttt
     1621 gaatgcgcaa agtacttcta gttctcgtgg cagttcttgt gagtgccgcg tttcttgttg
     1681 cggcagactt ccacatcggt gtcgtaacgg gtaccgtatc acagtccgaa gacgatctca
     1741 gaggggccga agccttgata aaaaaatatg gagatgccgc ctctggagga atgatcgtcc
     1801 atcttaccta cccggacaat ttcatgtcgg aacaggaaac agttatcgct cagatcaccg
     1861 gtctcgccga tgatcccctt atgaaagcta tcatcgtcaa ccaggccatc ccgggaacgg
     1921 tcgaaagctt caggagagta agagaagtaa gaccggacat cctcctcctg gcgggtcttc
     1981 cccatgaaga tcctccaatg ctggctgccg ccgctgacct atcggtgaac gccgactcca
     2041 tcgcccgcgg ctacctcata atctacactg ccaagcaact tggtgccgag aagttcatgc
     2101 acatctcctt cccgaggcat atgtcttacg aactgctatc caagagaaga gacatcatga
     2161 gagcagcctg cgaagagctg ggactggaat tcatcgacat gggatctccc gacccggtca
     2221 gcgacgttgg tgtggccggt gcgcagcagt tcatacttga gaaggttccc gcatggcttc
     2281 aggagttcgg aaacaagaca gccttcttct gtaccaatg
//
LOCUS       DAOOLY010000336         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_58020_length_2304_cov_5.181859, whole genome shotgun sequence.
ACCESSION   DAOOLY010000336 DAOOLY010000000
VERSION     DAOOLY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58020_length_2304_cov_5.181859"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLD15_11860"
     CDS             complement(<1..657)
                     /locus_tag="PLD15_11860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB64654.1"
                     /translation="MRKILLVLIAVAAIVTIGWACTTIIVTKGASVDGSVMTSHSADC
                     GACDFRYVYIPAADYEAGAQRAVYPFIEGYPRYVGKDMGPTYDTPGYPATEPLGYIDQ
                     VEHTYGYFDAVYGVINEHQLAIGECTCSAKVYAQAKAGECIFDVAALSRVAMERCTTA
                     REAIELMGALAVEYGYYGWGETLTVTDPQEAWVFEICATPDQKSALWAAKKVPDGEVF
                     V"
     gene            complement(719..1984)
                     /locus_tag="PLD15_11865"
     CDS             complement(719..1984)
                     /locus_tag="PLD15_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64655.1"
                     /translation="MKRYLVVLFAFFAVVACAGLTLSDLVVVGGDGDDVGNDIVLLAD
                     GGVVIAGYTTSSEGTFFSSNGQEDFLIARFDKDLELIWWKAYGGSKRDVAQALTATRD
                     GGFVLAGLTESSDGDVTGNKGLGDFWVISVSSTGELEWQKTFGGSGQDQAYDVVETPE
                     GNILVAGYTRSDNGDVIGYDWGEDFWIIELGRDGELLRQWLAGAYRSEDCAKKIAIGD
                     DGSIYVVGYYAFRDCNVRCNYVEEQMSVLKIGSDGVIQWFNQYGDYWPEAGIDLLVAD
                     NGNIVVVGEEESGVSLLTSGLGGKDFWIAYISPDGTAISSNNFGGSFTDIANGVVQVG
                     EDEFIVAGYSTSSDKDVVGNHGMADGWIIRVNTAGKILDKLAVGGSKDDAILSVFSRD
                     KTLYFTGYSTSSEMGSSGGRDLFIFTVIE"
     gene            complement(1999..>2304)
                     /locus_tag="PLD15_11870"
     CDS             complement(1999..>2304)
                     /locus_tag="PLD15_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64656.1"
                     /translation="GSEKHLEDIARVEEMAVEIKKENGLVMIIDIDVDDSGDDSIKAE
                     FARRLIPLTDAVVFAGSSSENYASALYEDSPIVLKLSIVVDLLIVFERDFGGGRCCD"
BASE COUNT          560 a          677 c          489 g          578 t
ORIGIN      
        1 aacgaagact tctccgtcgg ggactttctt ggccgcccag agagcgctct tctgatccgg
       61 tgtcgcacag atctcgaaga cccaggcttc ctgagggtcg gttactgtga gagtttcgcc
      121 ccagccgtag tagccgtact ctacagccag cgcgcccatc agttcgatgg cctctctcgc
      181 ggtggtgcat ctttccatcg ctactctgga aagagctgca acgtcgaaga tacactcgcc
      241 ggctttggcc tgcgcgtaaa cctttgccga acaggtacac tcgccgatcg cgagctggtg
      301 ttcgttgatt actccataga ctgcatcgaa gtaaccgtag gtgtgctcga cctggtcgat
      361 gtaacccaga ggttctgttg cagggtaacc cggtgtgtcg tacgtcgggc ccatatcttt
      421 gccgacatag cgagggtaac cttcgatgaa cggataaacg gctctctgtg cacctgcttc
      481 ataatctgcg gctggaatat aaacatacct gaagtcacag gctccacagt cagcagagtg
      541 gctggtcatg acagacccat caacggatgc gcccttggtg acaatgatgg tcgtgcaggc
      601 ccaaccgatc gttacgattg cggcaaccgc aatcaagact aacaggatct tacgcaattg
      661 aaacccctcc caactaaaat gtattgggga tataccccgg atttaaggtc gttagacctt
      721 attcaatcac tgtaaaaata aatagatctc ttcctccgga actccccatt tctgaagaag
      781 tggagtatcc cgtgaaatac agcgttttat ctcgcgaaaa taccgacagt attgcatcgt
      841 ccttagaacc tccgactgca agtttatcca ggatcttacc cgcggtattc actctaatta
      901 tccagccgtc ggccattcca tgattgccga ctacatcttt atcgctggat gttgaataac
      961 cggctacaat gaattcatct tctccaacct gtacgactcc atttgcgata tctgtgaaac
     1021 ttccgccgaa attattgctg gagatcgccg ttccatcggg cgatatgtac gcaatccaga
     1081 aatcctttcc accgagtccg cttgttaaca gggaaacacc tgactcctct tctcccacga
     1141 caacgatatt tccattgtct gcaacaagca gatctattcc ggcttcaggc caatagtctc
     1201 cgtactgatt gaaccattgt ataactccgt cgcttcctat cttcagaaca gacatctgct
     1261 cttcgacgta gttgcacctt acattgcaat ctctgaaagc gtagtacccg acaacataaa
     1321 tcgaaccgtc gtcgcctatg gcgatctttt tggcgcaatc ttcacttcta taagcaccgg
     1381 cgagccactg cctgagaagc tctccgtctc ggccgagttc tattatccag aaatcttcgc
     1441 cccagtcgta cccgatgaca tctccgttat cggatctggt gtatcccgcg acgagtatat
     1501 ttccttcggg agtctccacc acgtcgtacg cttggtcctg gccgcttcca ccgaaggttt
     1561 tctgccactc aagctcgccc gtcgaagaaa cgcttatcac ccagaaatcg cccaaacctt
     1621 tgttaccggt tacatctcca tctgaggatt ccgtcaatcc cgcaagtacg aaaccgccat
     1681 cccgcgtagc cgtgagcgcc tgggcaacat cgcgcttgct tccaccgtaa gccttccacc
     1741 atatgagttc gagatctttg tcgaacctgg cgataagaaa gtcttcctgt ccattactgg
     1801 aaaagaaagt gccttccgag gaagtggtgt aaccggctat cacgacgccg ccgtctgcga
     1861 gcagaacgat atcgttgccc acatcgtccc cgtctccgcc tacaacgacc agatcagaaa
     1921 gcgtcaaacc ggcgcaggct acaaccgcaa aaaaagcaaa taaaactacc agatatcttt
     1981 tcatccattt tcctcccatc agtcacaaca ccttccccca ccgaagtccc tctcgaaaac
     2041 aatcaataga tccaccacga tcgaaagttt caaaacgatt ggcgaatctt cgtacaaagc
     2101 agacgcatag ttctcgctcg agctcccggc aaatacaacg gcgtctgtaa gtggaatcaa
     2161 tctgcgggcg aattcggctt tgatcgagtc atcaccggaa tcatccacat ctatgtcgat
     2221 gatcataacc agtccgtttt ccttctttat ttcgactgcc atttcttcga ctctggctat
     2281 atcctccaga tgtttttcgg atcc
//
LOCUS       DAOOLY010000337         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_58286_length_2297_cov_5.353702, whole genome shotgun sequence.
ACCESSION   DAOOLY010000337 DAOOLY010000000
VERSION     DAOOLY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58286_length_2297_cov_5.353702"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLD15_11875"
     CDS             complement(<1..684)
                     /locus_tag="PLD15_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPB64657.1"
                     /translation="MEKFNGFTRATLDFLVDLGKNNNRAWMEASRERYREVLLDPFKA
                     LVNELGPFMEELDPFLETRPQVGKTISRISRDARRNRGRAPYRTNMWLTFKVPSPDWK
                     DSPGFFFELFPDSYRYGMGFYFPSRETMRKLIFELEENTRTFLEKTAILREGHFLIMG
                     EKYKRPRKKEGVPDELLVWYQMKDFYISVERGMDETLFSGEFADLLRSEFKALTPLYE
                     YFWEFRMKKE"
     gene            complement(728..1471)
                     /locus_tag="PLD15_11880"
     CDS             complement(728..1471)
                     /locus_tag="PLD15_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB64658.1"
                     /translation="MIDIHNHTIFSDGKDSVADVIQAAVERDLKIVGISDHFDPYLLR
                     QDVCLQPEKVSSYSREIERLKGSYPITVLSGLELGLQSTGISWPVGDFDYYIYSVHTV
                     PGFPDLRDMEDPWDIYLEEAAMAVELIDKPGFLGHLDFLRRHIPGSKPPSPGELMDIL
                     LKQIIACDVGLEFNTSGWMYRLGDPTPQPWVIERYLELGGRLITIGSDSHRAISVGKY
                     NNDALNLLRKLGISEVFYAVKDRYLSIPI"
     gene            complement(1491..>2297)
                     /locus_tag="PLD15_11885"
     CDS             complement(1491..>2297)
                     /locus_tag="PLD15_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB64659.1"
                     /translation="LQHFKKYLLDHDLDYTQVKRRQIEAFVKELSQGRYSTRKPSPST
                     VSRNLSTIRSFYTFLYISGVVPKVPTELIKNPKSRRRIPDYLSYDDVQKILASFKPTH
                     LGRRNRAIVASMYFCGLRVSEVCHLILSDLRLGENPAIRVQSGKGNRDREVPVNEQMQ
                     SIMLDYLEIRPTFPNSDSEEHLFVGTRGGPTARNLIPKMLNTHVKEVFPERKVNPHLF
                     RHSFATHLLQKGASIKTVQELLGHVNLATTSIYLHITDKEKREAVKLLSD"
BASE COUNT          506 a          598 c          554 g          639 t
ORIGIN      
        1 ctctttcttc atccggaact cccagaagta ttcgtacaga ggtgtcagcg ccttgaattc
       61 gctcctgaga aggtcggcaa attcgccaga aaagagcgtt tcgtccatgc ccctctccac
      121 ggagatgtag aagtctttca tctggtacca gacgagcagt tcgtcgggta cgccttcctt
      181 ttttctgggc cttttgtact tctctcccat gatcaagaaa tggccttcac ggagaatcgc
      241 cgtcttctcc agaaaggtcc tggtattctc ttccagttca aaaatcagct ttctcatggt
      301 ctcgcgcgat gggaaataaa agcccatccc gtaacggtag ctgtcgggga atagctcgaa
      361 gaagaagccc ggtgagtcct tccagtcggg ggatggcact ttgaaggtaa gccacatatt
      421 ggttctgtac ggagccctgc ccctgtttct cctggcgtcc cggcttatcc ggcttatggt
      481 ttttcccacc tgcggtctcg tctccagaaa gggatccagc tcctccatga agggcccaag
      541 ctcgttcact aacgccttga aagggtcaag aagaacctct ctgtatctct ctctgctggc
      601 ctccatccag gccctgttgt tgttcttccc tagatccacg agaaaatcga gggttgcccg
      661 ggtaaaaccg ttgaacttct ccatctcttc ctccttagct tactacaatt gttgaatcag
      721 aataagccta gattggtatc gacaggtacc tgtccttaac tgcgtagaag acctcgctta
      781 tccccagttt tcgcaggagg ttcagggcat cgttgttgta ctttccaaca cttatggccc
      841 tgtgagagtc cgaaccgatc gttatcagcc tgccccccag ttcgagatat ctttcaataa
      901 cccacggctg gggcgtggga tcgcccagtc tgtacatcca tcccgaggtg ttgaactcca
      961 gccccacatc gcaggctatg atctgtttca aaaggatatc catcagctcg cccggactgg
     1021 gtggtttcga accggggatg tgtcttctca gaaagtcaag gtggcccaga aaacccggtt
     1081 tatctataag ctccacggcc atggccgcct cttcaaggta tatgtcccag gggtcttcca
     1141 tatccctcag atcgggaaaa ccggggactg tgtggaccga atagatgtaa taatcgaaat
     1201 cgccgaccgg ccatgaaata ccggttgatt gcaatcccag ttcgagaccg gagagaacgg
     1261 ttattgggta gctgcccttc aggcgttcta tttctctgga atagctgctc accttttccg
     1321 gctgaaggca gacatcctgt ctcaaaagat atggatcgaa gtgatcggag attcccacga
     1381 tcttcaggtc tctctcaacg gccgcctgta taacgtcggc cacgctatct tttccatcgg
     1441 agaaaattgt atggttatgt atatctatca tatactactc ccgctggtat ttaatctgat
     1501 aacaatttca cagcttctct cttttcttta tctgttatat gcaagtatat acttgttgta
     1561 gcaagattaa cgtgtccaag tagttcctgt actgttttta tacttgcgcc cttttgaaga
     1621 aggtgagtcg cgaagctgtg cctgaataaa tgcggattta cttttctttc aggaaatacc
     1681 tctttaacat gcgtattcaa cattttgggg atcaagtttc tggccgttgg ccctcccctg
     1741 gttccgacga agaggtgctc ctcgctgtcg ctgttgggaa aggtgggcct gatctcgagg
     1801 taatcgagca ttatcgattg catctgttcg ttaaccggca cttcacggtc cctgtttccc
     1861 ttaccgctct gaacacgtat cgccgggttc tctccgagtc gcaggtcgct caggatgaga
     1921 tggcagacct cgctcacacg taaaccacag aaatacatgc tggccactat cgcccggttc
     1981 ctccttccca gatgggtcgg tttgaaagag gccagtatct tctgcacatc gtcgtagctg
     2041 agatagtccg gtattctgcg ccgtgatttg gggtttttta tcagctcggt tggaactttt
     2101 gggaccactc cactaatgta gagaaaagta tagaaggaac ggatggtcga caagttcctt
     2161 gaaacagtcg aaggcgaagg ctttctggtg gaatacctgc cttgggagag ctccttcacg
     2221 aaggcttcga tctgtctgcg cttgacctgt gtataatcca ggtcgtggtc gagaaggtac
     2281 ttcttgaagt gctgaag
//
LOCUS       DAOOLY010000338         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_58293_length_2297_cov_3.700714, whole genome shotgun sequence.
ACCESSION   DAOOLY010000338 DAOOLY010000000
VERSION     DAOOLY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58293_length_2297_cov_3.700714"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(534..>2297)
                     /gene="oraE"
                     /locus_tag="PLD15_11890"
     CDS             complement(534..>2297)
                     /gene="oraE"
                     /locus_tag="PLD15_11890"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HPB64660.1"
                     /translation="RPINFHSYVSGVAGPEIAVLFAEEGVNGAHQDPQYNVLYRGINP
                     IRSFVDAAVAKKIMASVDMLQIDGAHNANASSKSAWKVMPELLVQHAINCAFSTRVGM
                     KKDLIALSTVPPVTAPAPEFRLNFIYALTLRELFKEYRFRAQMNTRYIESDLLDATRI
                     HVLDTLISRLTKADIQSTITPDEGRNVPWHINSIRGIETAKHTLLALDGIKDLVKVNE
                     EAVRPQIREMKMRAILMMEEIIESGGYFEAVEQGFFVDNGQYPERNGDGITRLKEGGI
                     GAGTVVARESDYYAPVCEHFGYNNMPGSLNRPDEPIGRCSLEDRSKIKYIDELDESDN
                     VNLRITTQLEDIKAGLIGPEVEWWGDGWVQLDLTIPDDPDHAEAAALEIARRMGLLEA
                     TVISKTVLHPVEGTYLELKARVPFKIERESLELPKKPDLLEPEEITGYLKEIPMKVVA
                     GTVGNDEHSVGIREILDIKHGGIEKFGFKYTYLGTSVHPEKFIDAAIETGADAILVST
                     IITHNDVHVDNMKKIAELSIEKGVRDRLILVSGGTQITNELAVECGMDAGFGRGTKGI
                     NVASFLVKKKREMREADGSVS"
BASE COUNT          450 a          664 c          536 g          647 t
ORIGIN      
        1 ttcccagggc acgctcgccg ctaccggcca cattacgccc tggcggcccc gtccgggccg
       61 tattaaccgc tagcatcagg atattccggg cggcgttcac gtcgcgctca agcgtgaggc
      121 cgcagtcagg acaaacatgt acgcgaatcc tcaaatcttt cggtacaatt gcaccacact
      181 cagaacacgc ctgcgaggtg catgccggat tcacgatcac aattcgcgta ccggcacttt
      241 cagccttgta cgcgagcagt ggtacttgac cttgatctct cccacattct ggacgtagag
      301 cattacgcgc ccgccaggtc gcagcttgca cccgtcaccg tgccgttatc ccgtagcgat
      361 cctattcggg atcccgttct tatcgccctc tattcctctc ttcatctctg cacttcgcgc
      421 ggtcaataaa taaaacacga gaaagagata atcctcgtat ctcttcagtc tttctctttc
      481 tctctcgctc tctcgaagga cggttcttaa cgctctttca cttatcgctc tttttaactt
      541 acacttccat ccgcttctct catctctctc tttttcttaa ccaggaaaga tgcaacgttt
      601 attcccttgg ttcccctacc aaaacccgca tccataccgc attccacagc cagttcgttg
      661 gttatctgcg tgcctcccga gacgagtatg agtctgtccc tgacaccctt ctcgatcgaa
      721 agctccgcaa tcttcttcat gttatcaacg tgaacatcgt tgtgggtgat tattgtggag
      781 accagtatgg catcggctcc cgtctcaata gctgcatcga tgaacttctc aggatgtacg
      841 ctcgtaccaa gataggtgta tttgaaaccg aacttttcta taccgccgtg ctttatatcg
      901 agtatctccc ttatgccaac cgagtgctca tcatttccga cggtgccggc gactaccttc
      961 atgggaatct ctttcaggta gccggttatc tcttcgggtt ccagtaggtc gggtttcttt
     1021 ggtaattcca ggctttctct ttcaattttg aaaggcactc tggctttcag ttctaggtac
     1081 gttccttcga ccgggtgaag cacggtcttg cttattacag tggcctccag cagacccatt
     1141 ctccttgcga tttccagagc cgcagcctcg gcatgatccg ggtcgtctgg tatagtgagg
     1201 tccagctgta cccagccgtc gccccaccat tccacttccg gccctatcag tccagccttt
     1261 atgtcttcga gttgtgtagt aattctcagg tttacgttgt cgctttcgtc tagctcgtct
     1321 atgtacttga tcttgcttct gtcttccaga gaacaacggc caattggttc gtcgggcctg
     1381 ttcaggcttc ccggcatgtt gttgtaacca aagtgctcac agacgggtgc gtaatagtcg
     1441 ctctctctgg ccacaacggt gccggctccg atgccaccct ctttcaacct cgttatcccg
     1501 tcgccatttc gctcggggta ctgtccgttg tcaacgaaga aaccctgctc gacggcttcg
     1561 aagtagcctc ccgattctat aatctcctcc atcatcagta tagcccgcat cttcatctct
     1621 cttatctgcg gcctcacagc ctcttcattg acctttacga ggtcctttat cccgtcgagg
     1681 gccagaagag tgtgtttcgc tgtttctata ccccttattg agtttatgtg ccagggcacg
     1741 tttctgcctt cgtcgggggt tattgtggat tgtatatcgg ctttggtcag acgcgatatc
     1801 agtgtatcca gaacgtggat acgtgttgcg tccaggagat ccgactctat gtagcgggtg
     1861 ttcatctggg ccctaaaacg gtattctttg aagagctctc tcagcgtaag cgcatagatg
     1921 aagttcaatc tgaactctgg ggcgggagcg gtcacgggcg gcaccgttga gagggcaatc
     1981 agatcctttt tcataccgac tctggtcgag aaggcacagt ttatggcatg ctggaccagg
     2041 agttccggca tgactttcca ggcactcttt gaagaggcgt tggcgttatg cgctccatcg
     2101 atctggagca tatcgacact ggccatgatc ttcttcgcga ctgccgcgtc aacgaaggat
     2161 cttatggggt tgatccccct gtaaagaacg ttgtactgag gatcctggtg agcgccattg
     2221 actccctcct cggcaaagag aacggcgatc tctggaccag ccacaccgct gacgtaagag
     2281 tggaagttta taggacg
//
LOCUS       DAOOLY010000339         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_58651_length_2287_cov_3.986559, whole genome shotgun sequence.
ACCESSION   DAOOLY010000339 DAOOLY010000000
VERSION     DAOOLY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58651_length_2287_cov_3.986559"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="PLD15_11895"
     CDS             complement(<1..1721)
                     /locus_tag="PLD15_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HPB64661.1"
                     /translation="MGRKTGAIVNRDLFDKILNFLIEGIIVIDASGKIIFINEEALKI
                     VGLTRDRAENFLIVDTIPNTRLHIVLQSGVPEINRIQYLRDKTIVTSRFPLLDESGKV
                     YAAMAVFRDMTSVEKMAEEVINHKEMEALLTGIIDATYDAISVADEKGKIVLVNKAYT
                     RITGMSAEYVIGKMATIDIAEGSSLHMEVARIREPIFNAKLKVGPGKKDVIVNVTPLL
                     VRGKFKGSVGVIHDISEIERLARELEDARRLLRYVKARYTFDDIAGSSPLMKIAKEQA
                     HKVSRTRATVLLNGQSGTGKELFAHAIHNSSDRRDASFISVNCAALPETLLESELFGY
                     VEGAFTGARRGGKKGLLQEANGGTVFLDEVGKMNLPVQSKFLRFLQDHEIKPVGGNKS
                     MKLDVRIIAATNLELDKLVEEGRFLPDLYYRLNVVPIVIPALKNHSEDIPEIVHTIVM
                     RLNQEYGRMVEKIEPAALDALQKHNWPGNVRELENVIGRAIINMEPDEHIIKASHLQG
                     LSTIPEKRDPLPEGDLEKMMNDYERCIIVAALDKNDWNKTATARELGISIRSLYYKLE
                     RHVIRDNT"
     gene            complement(1678..>2287)
                     /locus_tag="PLD15_11900"
     CDS             complement(1678..>2287)
                     /locus_tag="PLD15_11900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HPB64662.1"
                     /translation="EGPGCKLIYEDFLKADLGSISGDVAYVANIPYYITSPIIERIMF
                     EGPRFSKALLMVQKEYATRLTASVGTKEYGVLTVNVNTFATVRELFQVSKSEFIPRPE
                     VDSMAIELRLLERPEELPDRDGYRAFVRRCFSQRRKKLTNNLKCLVSSPGELLLSCGI
                     DPSVRAEELSVGQFTELYRLYTLQREAERGPKNRGYREPGSL"
BASE COUNT          472 a          601 c          561 g          653 t
ORIGIN      
        1 gtgttgtcac gtattacgtg tctttcaagc ttatagtata gactcctgat gcttattccc
       61 agttctctcg cggttgccgt tttattccag tcgtttttgt cgagcgcggc gacgatgata
      121 catctttcgt agtcgttcat cattttctcg agatcgccct ccggcaaggg atcgcgtttt
      181 tccgggatag ttgagagtcc ctgaaggtgc gacgctttga ttatgtgctc gtccggctcc
      241 atgttgatga tagccctgcc tatgacgttt tccagttccc tgacgtttcc gggccagttg
      301 tgcttttgaa gtgcatccag ggctgccggt tcgatttttt cgaccattct gccgtactcc
      361 tggtttaggc gcatgactat cgtgtgtact atctcgggta tgtcttcgga atgatttttg
      421 agtgccggga tcactatcgg caccacgtta agccggtagt atagatcggg gaggaacctc
      481 ccctcttcca ccagtttgtc tagctccaag ttagtcgcag caattatccg aacgtccagt
      541 ttcatcgatt tgtttccgcc tactggctta atttcgtggt cctgaaggaa ccttaaaaac
      601 ttcgactgca ccgggaggtt catctttccg acttcgtcga ggaagactgt tccgccgttc
      661 gcttcttgca gcagcccttt cttgccaccg cgtctggcac cggtgaaggc accctcgacg
      721 tagccgaaca attcggactc gagaagcgtt tcgggaaggg cggcacagtt gacgctgatg
      781 aaactcgcat cgcgcctgtc gctggagttg tgtatggcat gggcaaagag ctcctttccg
      841 gttccactct gcccgttcaa caggacggtg gcgcgtgttc tggaaacctt gtgggcctgt
      901 tcttttgcaa ttttcatgag cggcgaggag ccggcgatat catcgaaggt gtacctggcc
      961 ttcacgtatc gcagcagccg ccttgcatct tcgagttccc tggcgagacg ttcaatttcg
     1021 gagatatcgt ggataacgcc cacgctacct ttgaacttcc ctctgacaag aagtggcgtc
     1081 acgttcacta taacgtcctt tttccccggt ccgactttga gcttagcgtt gaagataggt
     1141 tctcttatcc tcgccacttc catgtgcagc gacgaacctt cggcaatgtc gatggtagcc
     1201 atctttccta taacgtattc ggcactcatg ccagtaattc tcgtgtaagc cttgttgacc
     1261 agcactattt tgcccttttc atcggcaacg gatatggcgt cataggtggc gtcgattata
     1321 ccggtcaaca gagcctccat ctctttgtga ttgatgacct cctcggccat cttctccacg
     1381 ctcgtcatgt cgcgaaagac agccatggca gcatagacct ttccgctctc atccagaaga
     1441 gggaagcggg acgttacgat ggttttatct ctcagatact gaatcctgtt gatttcggga
     1501 acaccgctct ggagaacaat atgtaatcta gtattgggta tggtgtcgac tatgaggaag
     1561 ttttccgccc ggtccctggt gagtccgact atcttcaaag cctcttcatt aatgaaaatg
     1621 attttaccgg aggcgtcgat gactattatg ccttctatca gaaagttgag tattttgtca
     1681 aagagatccc ggttcacgat agcccctgtt tttcggccca cgctcagctt ctctttgaag
     1741 ggtgtaaagc ctataaagct ctgtaaattg gcctaccgaa agttcctcgg ccctgactga
     1801 gggatctatt ccacaggaga gaaggagctc tccgggcgaa ctcactaagc acttgaggtt
     1861 gttggtcagt ttcttcctcc tttgagagaa acaccgtctc acaaaagccc tgtacccgtc
     1921 tctatcgggc agctcttcag gcctctcgag gaggcgaagc tctatagcca tcgaatctac
     1981 ctcggggcgg ggtatgaact cgcttttcga aacctggaaa agctctctta ccgtggcgaa
     2041 agtgttcacg ttgactgtga gaaccccgta ttctttggtt ccgaccgacg ccgtcagtct
     2101 tgttgcatac tccttctgca ccatgagcag cgctttagaa aagcgcggac cttcgaacat
     2161 tatcctttcg attatcggtg aggttatgta gtaaggaatg ttcgcaacgt aagccacatc
     2221 cccggaaatt gagccgagat cggcctttaa aaaatcctcg tatatgagtt tgcaaccagg
     2281 accctca
//
LOCUS       DAOOLY010000340         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_59144_length_2274_cov_2.987382, whole genome shotgun sequence.
ACCESSION   DAOOLY010000340 DAOOLY010000000
VERSION     DAOOLY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59144_length_2274_cov_2.987382"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLD15_11905"
     CDS             <1..396
                     /locus_tag="PLD15_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64663.1"
                     /translation="GKFTYAYRFNVLWLDSWPIVSGFFGFPYGPDSVVDLIGVKYERP
                     ELSLTGLLEYVRTGSIDINSTYFPPFDTDWYGPKEPIKRSVSLFLKGLLAIGNDAAIF
                     ASAKLMFKERFYFYMNLGFIKTFTILGGL"
     gene            396..1139
                     /locus_tag="PLD15_11910"
     CDS             396..1139
                     /locus_tag="PLD15_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64664.1"
                     /translation="MKKILLPILLVVSIALFANQGELILKGTLEHDLLLGRNLIEGKT
                     LFTPNTAIPESFASVQMGTSPLKSNNVTEVSFRLTPQFLIHNFEPFKETYWLESKVPL
                     YLRYRYDTLKPWTVLAFESDISQNLKGYINLDVPKDYRSYISHGIDFNAKPYMNFPVE
                     IFEGKLQAIDMSWPSFGYISYGNGGFYASLGRYKIAWGPMRNGLMISDASMYYDNVSS
                     TYTTALGNGGRFTYSFVFISSNSMLNSEE"
     gene            1206..2027
                     /locus_tag="PLD15_11915"
     CDS             1206..2027
                     /locus_tag="PLD15_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HPB64665.1"
                     /translation="MGHRFDVQFAPWVRLGVGEVNLVGGKHPDLNDINPFVIFHNTYG
                     EDFSNVMASVDLSVVPFKGVELYGEFVSDDVAFGATEAGARGKPTALAYGGGIRYSTQ
                     FKEIMILASIELYHTDTWMYNRWQPLLIFTNRIYTKSELPGSRDFTDYPLGFKYGPDM
                     NAFSVSARGFFQNGMTVSLVYDHFKQGEVTLKTPYLNMDDPSDDPGEFTDPEDWSGPV
                     GETVSANIVTLAIAFPYRDFTFGGDFSLYFGDYFKKYGGYEKAIFEIRPYISYVF"
     gene            complement(2080..2226)
                     /locus_tag="PLD15_11920"
     CDS             complement(2080..2226)
                     /locus_tag="PLD15_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64666.1"
                     /translation="MGIYDRDWYKESQKEEGTASRKGDRFTKGLITGLVIGFVIGFLV
                     FGFI"
BASE COUNT          560 a          602 c          552 g          560 t
ORIGIN      
        1 ggtaagttca cgtacgctta caggttcaac gtactctggc tggactcatg gcccatagta
       61 tcggggtttt tcggatttcc ttacggtccc gactctgtcg tagatctgat aggcgtgaaa
      121 tacgaaagac ccgagctgtc cctcacgggc cttctcgaat atgtgagaac cggttcaata
      181 gatatcaatt ccacgtattt tccgcccttc gacacagact ggtacggtcc gaaggagcct
      241 ataaagcgct cagtatcgct cttcctgaag gggttgctgg cgattggaaa tgacgcggcc
      301 atcttcgctt cagccaaact catgttcaag gagcgttttt acttctatat gaatctcggt
      361 ttcataaaga catttacaat tctgggaggt ttatgatgaa aaagatcctt ctgccgatac
      421 tcctcgtagt ctcgatcgcg cttttcgcaa atcagggtga acttatcctg aagggtacgc
      481 tcgaacacga tctgcttctg ggaagaaacc tgatcgaagg aaagactctc ttcacaccca
      541 acaccgccat tcccgaatcg ttcgcgtctg ttcagatggg tacttccccg ttgaaatcga
      601 acaacgttac cgaggtctct ttcaggctca ccccgcagtt tctgatccac aatttcgagc
      661 ctttcaagga aacctattgg ctggagtcca aagtacccct ctatctcagg tacaggtacg
      721 atactctgaa accatggacg gtactcgcct ttgaaagcga tatctctcag aacctgaagg
      781 ggtatatcaa tctggacgtt cccaaggatt acagaagcta cataagccac gggatcgact
      841 tcaacgccaa gccctacatg aactttcccg tagagatctt cgagggaaaa ctacaggcga
      901 tcgatatgag ctggccatcc ttcgggtaca tctcttacgg caacggcggc ttctacgcgt
      961 cgctcgggag gtacaaaata gcctggggtc cgatgaggaa cggcctaatg ataagcgacg
     1021 cttccatgta ctacgacaac gtatcatcga cctacacgac ggcgctggga aacggcggtc
     1081 gcttcactta ctcgttcgtt ttcatctcct cgaactccat gttgaactcc gaagaataga
     1141 taaaacagga caaagtctgg gacctcaacc agagaagacc ctacaccgaa ggggcgaaaa
     1201 cgataatcgg acaccgtttc gatgttcagt tcgcaccctg ggtaaggctc ggagtcggtg
     1261 aggtcaacct ggtgggcggc aagcatcccg acctgaacga tattaatccc ttcgtgattt
     1321 ttcataacac ttacggcgaa gatttctcca acgttatggc gagcgtggat ctttcggtgg
     1381 tgcccttcaa aggggtggag ctttacggag agttcgtcag cgacgatgtg gccttcggag
     1441 ctaccgaagc gggagccagg ggaaagccga ccgcgctggc ttacggcggg ggtataaggt
     1501 attcgacaca gttcaaggaa atcatgatcc tggcttcgat cgagctttat cacaccgaca
     1561 cctggatgta caacagatgg cagccgctcc tcatcttcac gaaccgtatc tatacgaagt
     1621 ccgagttgcc gggcagccgg gattttacgg attatccgct gggcttcaaa tacggtcccg
     1681 atatgaacgc tttttctgta tcggccagag gtttcttcca gaacggaatg actgtcagcc
     1741 tggtttacga ccacttcaag caaggagagg tgacactcaa gaccccctac ctgaacatgg
     1801 acgatccatc ggacgacccc ggtgagttca ccgatccgga agactggtcc ggcccggtcg
     1861 gcgaaacggt tagcgcaaac atagtgaccc tggcgatcgc cttcccgtac cgcgacttca
     1921 ccttcggagg agatttctcg ctttacttcg gtgattactt caagaagtac gggggttacg
     1981 agaaagcgat cttcgaaatc agaccttaca tctcttacgt attctagagc tgttctgtag
     2041 atagagaaca tgccgcccca ggggcggcat gtcattggat cagatgaaac cgaaaaccag
     2101 aaaacctatc acgaaaccga tcaccagacc ggttatcaat ccttttgtga atctgtcgcc
     2161 ttttcttgag gcggttcctt cttctttctg gctttcttta taccagtccc tgtcgtagat
     2221 tcccaattct taccactcct ttcgacaaga acgaattcct tccagaagcg tttg
//
LOCUS       DAOOLY010000341         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_61005_length_2226_cov_5.152004, whole genome shotgun sequence.
ACCESSION   DAOOLY010000341 DAOOLY010000000
VERSION     DAOOLY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61005_length_2226_cov_5.152004"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(408..878)
                     /locus_tag="PLD15_11925"
     CDS             complement(408..878)
                     /locus_tag="PLD15_11925"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPB64667.1"
                     /translation="MPRVSTIGLLKRSEQPTLVVRTATSVQNLPGLIGKCYGEIAAYL
                     GELGEFPADIPFVAYHNMDMQNLDVEIGFPVSSKLSGRGDIVQGSIPEGLNVFSIFMG
                     PYAQMESVYSEMAEWITNNGFISTGVVYEHYYNGPEFPESQHLTKIVMPLKKPE"
     gene            complement(1032..1565)
                     /locus_tag="PLD15_11930"
     CDS             complement(1032..1565)
                     /locus_tag="PLD15_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64668.1"
                     /translation="MALYPQSLASAVHTFDIIGRGAYLYRKDGTMTVICHPVDEEFFC
                     WMMNTDGSAAQHDRSGSMYRDRFVVADGSYPVFVMNVRNNTDHAITFGSSDITISEMK
                     VSILELRNESFEIAETITVADNSEIRVFIYTDETTVKNRLELEKLLVDFREFSFDFRN
                     NPAWDFLKNDIVIYLKY"
     gene            1879..>2226
                     /locus_tag="PLD15_11935"
     CDS             1879..>2226
                     /locus_tag="PLD15_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64669.1"
                     /translation="MRKLFMLLLLSICFFTAFAFAAQTLVVAFMGDPIWFNPCAKTDD
                     YGASVNRNIFDQLVAQDVNYNLVPQLATSWEVSEDGKEYTFHLRKGVKWHDGEAFTSR
                     DVKYTLESIVNNKG"
BASE COUNT          540 a          525 c          531 g          630 t
ORIGIN      
        1 tttttcattt cttacacctc catatgattt ttggtacatt ctcagtctac ggcgtgtttc
       61 ttagagtccg cttaacagcc gcttagagat tgcttagaaa acggaaccgt ggtttctgat
      121 gggaagttgg cggttggaat agcgaagaac cagttgcaag ttataggtat aggggggagc
      181 agaaagaaat cccgtgtcgg ggcacgggga tgacagtctt acaattttct cttcatgcgg
      241 accttaatcc ttcatcttgc tattacccac aacccccaac gcacaaccga cagctgttct
      301 tagagagaga aagtccccgg agacgcggat ttgaatagac agcttttacg tttcgtgtat
      361 cgcccgcctg gatcggggtc accgaaagtc aatcggtgtg gtgtcgttta ttcaggcttt
      421 ttaaggggca tcactatctt tgtgaggtgc tggctttctg gaaattccgg accgttgtaa
      481 taatgctcat aaacgactcc ggtagaaatg aatccgttat tcgttatcca ttcggccatc
      541 tcgctgtaca ccgattccat ctgggcgtaa ggacccatga agatggagaa aacgttcaac
      601 ccttcgggaa tcgatccctg aacgatgtcg ccccttccgg agagtttcga tgagacgggg
      661 aagcctatct cgacatcgag attttgcata tccatgttgt gataagccac gaatgggata
      721 tctgccggaa actctcccag ctcgccgaga taagccgcga tctctccata gcactttcct
      781 atcagtccgg gcagattttg aacgctcgtt gctgtacgta ctacaagcgt tggctgttcg
      841 ctccgtttca gtaatcctat cgtagatacc cttggcattt ttgtcccccc tttgcacttt
      901 gtttcgaagt gacctttgta gtgtcgattt ctctaatctc taacctgttc gagtcgatta
      961 tgtgtggcaa ctacattcag aatactacat ttctctggaa tttccacaag cccgacagat
     1021 cgagtatccg gtcagtattt caggtatatc actatgtcgt ttttgaggaa gtcccaggcg
     1081 gggttgttcc gaaagtcgaa ggagaattcc ctgaaatcaa ctagcagctt ctcgagttcc
     1141 aagcggtttt ttactgtcgt ctcgtcggtg tatatgaaga ccctgatttc ggagttgtcg
     1201 gcaacggtaa tagtttcggc aatttcaaag ctttcgttcc ggagttcgag gatcgagacc
     1261 ttcatttcgc ttatggttat gtcggaggag ccgaaagtga tcgcatggtc tgtgttgttt
     1321 ctgacgttca tcacgaaaac gggataactt ccgtcggcca ccacgaaccg gtctctatac
     1381 atagatcccg agcgatcgtg ctgggcggcc gaaccgtcgg tgttcatcat ccagcagaag
     1441 aactcctcgt ccaccggatg gcagatgacg gtcattgtac cgtctttccg atacagatag
     1501 gcgccccggc cgatgatatc gaaagtatga accgcgcttg ccagagactg cggatacagt
     1561 gccatcaacc gactaatgaa cacgatctgt aaaagcaaga cgagtatcca gttcaggggt
     1621 ctttctagtc gtcctctcat gagaatataa tacaccgatt agcaagatta aagcctgaga
     1681 aacgatattc gccaattaaa gttgtttatg atcttctatc gtgcatatac tttctataac
     1741 tctccaaaag ttccgatttc ataactttat aattaccaat gattcggtcg gtcgttgttg
     1801 tgatagtatg attagcaaag ttgggcttat tcttcagggt tgtttttcca catcatttct
     1861 accaagggga ggtgctctat gagaaagttg tttatgctcc tgctactttc catctgtttc
     1921 ttcacggctt ttgcgttcgc ggctcaaaca ctggtggttg ccttcatggg tgatcctata
     1981 tggttcaatc catgcgccaa aaccgacgac tacggcgcgt cggtgaacag aaacatcttc
     2041 gatcagctag ttgcacagga tgtcaattac aatctggttc cacagctggc gaccagctgg
     2101 gaagtctccg aagacggtaa ggagtacacg ttccacctga gaaaaggcgt gaagtggcac
     2161 gatggagaag ccttcacttc cagagacgtc aagtatacgc tagagtctat cgtcaacaac
     2221 aagggt
//
LOCUS       DAOOLY010000342         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_61305_length_2218_cov_4.281553, whole genome shotgun sequence.
ACCESSION   DAOOLY010000342 DAOOLY010000000
VERSION     DAOOLY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61305_length_2218_cov_4.281553"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLD15_11940"
     CDS             complement(<1..326)
                     /locus_tag="PLD15_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB64670.1"
                     /translation="MNRTYPIFKESELLGSVLEKVREYGLSTIITTTQNGRLSGIVSV
                     DEIDSNDYEGELAGIVREPAFYCTESDFIEDAALILIESHDYLLPVVDEELIVTGVIS
                     VFEILEG"
     gene            442..1572
                     /locus_tag="PLD15_11945"
     CDS             442..1572
                     /locus_tag="PLD15_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB64671.1"
                     /translation="MFHGYVKLLELATNLFTMYRWNNMPTLVRTNEAENAFVSAQYCL
                     LMSEMSALHGLELDDNKLFQRLVLKELPKCLLSDISVDTKILIKSLSPEKWTAVFSKT
                     VEEVVQYFPSKRRGEFFASMVDTKDASTEGRIIQTGDLLSARLEAEIHGRYFPDFFAR
                     PLKDLEGRLESFKDFQPYGLLVNSDWVKRYADALVVLLRAVRWNRLNRNVPTTVAGHS
                     FYVTLTSYILSSMEIENGEKIDPVEVVKRALLHDIPESMTGDIITPTKKKVPGFEEVI
                     SQVEEQMVSDNLLRGMPDELVKELKSRMLDPFATTEGELVRAADQFAATVECLMEIRS
                     GNTQPAFRDALHGMLDDLSSSTFESVRFVTESFRWGLDWAGR"
     gene            complement(1573..>2218)
                     /locus_tag="PLD15_11950"
     CDS             complement(1573..>2218)
                     /locus_tag="PLD15_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB64672.1"
                     /translation="NPTGAIYPESFLRELAALSFERDFMVISDEVYEKLVFDAPHFSI
                     GSVPGMKERTAVINAFSKTYAMTGWRVGYSATSLKLSKAIGKIQSHLVSNVNTMAQYA
                     ALKAFEVDTSEMVRIFKERRDFVVSRLNAMNMVFNDPSGAFYVFIDVRPYLGGRFKDS
                     QEFAIGLLEEKKVGMVPGSAFICEGFIRMSYSSSMEDLKEGLDRFESFLSEGKR"
BASE COUNT          521 a          628 c          542 g          527 t
ORIGIN      
        1 ccttcgagaa tttcgaagac gcttataacg cccgtcacga tcagctcttc atcgacgacc
       61 ggcaagagat aatcgtggct ttcgatcaat atcagcgccg cgtcttctat gaagtccgac
      121 tcggtgcaat agaaggcagg ctctctgact attccggcca gttcgccttc gtagtcgttg
      181 ctgtctattt catctaccga aacgatcccc gaaagtcttc cgttctgagt cgtcgttatt
      241 atcgtagata gcccgtactc cctaactttt tcgagaacac ttcccagcag ttcgctttct
      301 ttgaaaatgg ggtaagtcct gttcatccat ttttcgatgt tcactcttct cgcctctctt
      361 caattacccg gaatttttat cagtttccgt catccataac ttataattga acacagtata
      421 ctctcgattg ggggaataac ggtgtttcat ggttacgtaa aactccttga gctggcaacg
      481 aatctcttta cgatgtacag atggaacaac atgccgacgc tcgtcagaac caacgaggcg
      541 gaaaacgcct tcgtgtcggc acagtactgc ctgttgatgt cggagatgtc ggccctccac
      601 gggctggaac tcgacgacaa caagctcttc cagcggctgg tcctcaaaga actacccaag
      661 tgtctcctct ccgatatctc ggtcgatacg aagatactca taaaatcgct gtctccggaa
      721 aaatggaccg ccgtcttctc caaaaccgtc gaagaggtcg tccagtactt tccctccaaa
      781 agaaggggag agttcttcgc ctccatggtc gacacgaagg acgcttcgac cgaaggccgt
      841 ataattcaaa cgggtgatct gctttcggca aggctcgagg ccgaaattca cggcaggtac
      901 ttcccggatt tcttcgccag acccctcaag gatcttgaag ggagactgga gagtttcaag
      961 gatttccagc cttacggttt gctagtgaac tccgactggg ttaagcgata cgccgacgcc
     1021 ctggtcgttc ttttgcgcgc tgtcagatgg aacaggctca acaggaacgt tcccacaacg
     1081 gtcgcgggcc attccttcta cgtcactctc acatcttaca ttctctccag catggagata
     1141 gagaacggag agaagatcga cccggtggaa gtcgtcaagc gcgccctcct gcacgacata
     1201 cccgagagca tgaccggaga tataatcacc ccgacgaaaa agaaggtccc cggattcgaa
     1261 gaggtgatat cccaggtcga ggaacagatg gtcagcgaca acttgctccg cggtatgcca
     1321 gatgaacttg tcaaagagct caaatcccgg atgctcgatc ccttcgccac gaccgaaggg
     1381 gaactcgtaa gggcggccga tcaattcgcg gccacggttg agtgtcttat ggagatccgt
     1441 tccggcaaca cccagccggc cttcagggat gccctgcacg gaatgctcga cgacctctcc
     1501 tcttcgactt tcgaaagcgt ccggttcgta acggaatcat tcagatgggg gctcgactgg
     1561 gccggccggt gatcaacgct tcccttcgct gaggaatgac tcgaacctgt ccagcccctc
     1621 cttcagatct tccatggagc tggagtacga cattcttatg aagccttcac aaatgaaggc
     1681 gctacccgga accattccca ctttcttctc ttccagcaat cctatggcga actcctgcga
     1741 atctttgaat ctacccccga ggtaaggccg gacatctatg aagacataga aggcgccgga
     1801 cgggtcgttg aaaaccatgt tcatggcgtt cagcctggaa acgacgaaat ccctccgctc
     1861 cttgaagatc ctcaccatct cggaagtgtc gacttcgaag gccttcagcg cggcgtactg
     1921 ggccatcgtg ttcacgttcg agaccagatg gctctgtatc ttgccgatgg ccttagatag
     1981 cttcagagaa gtggccgaat aaccgaccct ccagcccgtc atcgcatagg tcttcgagaa
     2041 ggcgtttata acggccgttc tctctttcat tcccggtacg gagcctatgg agaagtgcgg
     2101 cgcatcgaaa acgagctttt catagacctc gtcggatatg accataaaat cccgttcaaa
     2161 gctgagcgcg gccagttccc tgaggaagct ttccggatat atggcacccg tcgggttg
//
LOCUS       DAOOLY010000343         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_62809_length_2183_cov_2.559211, whole genome shotgun sequence.
ACCESSION   DAOOLY010000343 DAOOLY010000000
VERSION     DAOOLY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62809_length_2183_cov_2.559211"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1713
                     /locus_tag="PLD15_11955"
     CDS             430..1713
                     /locus_tag="PLD15_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB64673.1"
                     /translation="MLRKLAGYKYMIATLLYASLFFTQSLTLTWTVPYIVQALILNTA
                     IMLGIYAFRGFDNSTTTSMNSCFVSFLSGTILGVVFAMIPILFFHARLPRFSIAITAA
                     LSIFIFPFIGCILMRYTIKHMPPRRYLVIGREEELSPILEEVRKASMNKIQIYSYLNP
                     SPATLAAAIEIETARPYDAILIGDPQLARGVEHILNEAITKNTDIEYLPTLVENTLHR
                     IPIEIITIFKDYYQIIFSQARYSRRIRVMDILFSGALLVLFSPFILFSIVYILARDGR
                     PVFYSQPRHGLNAKIFRVYKFRTMNDPEEEETKPTITRSGKLLRMTRLNEIPQLLSVL
                     KGDMSLVGPRPDIPSTYEYCTKEIPFYRYRTKIMPGITGHAQVLYRYVDKLEYDTFAR
                     RLSYDLYYVKNMDFRLYLSVMLRTAETMVFRRGAK"
     gene            1713..>2183
                     /locus_tag="PLD15_11960"
     CDS             1713..>2183
                     /locus_tag="PLD15_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB64674.1"
                     /translation="MTEILVSNVEKWLTPHLERVLAIKYTEQLKHSHFDSFDPERPYI
                     IGFEYIGEFTDMGSLGDVLHLASRTGRVKRIIYFSSYGVYNPGRKQFNESDVVCPMNF
                     VGARSAMLEDILVYLANRYSLDLTILRLFNPYGPYQLSPYVVPTVLERIATSGTV"
BASE COUNT          566 a          642 c          466 g          509 t
ORIGIN      
        1 aggtgtgaag atcaagatct tctctctcct cttctcgtga aaatggggac tgacgattca
       61 cctcacgtct gtccccgatt ttcgcgtctc tctcttaatc tcgtgttttc gatactcctc
      121 tcgcctctca cctcccacga tctctaaaaa ttttacattt cccccactct ccttaccttg
      181 actgacatac gaaaacttaa tataattaga aacataagcc tataaaacgg cctggatagt
      241 cataaacgga cttgggatat atttgaaggt aagtttgtta gtgttaacac gatgaatcga
      301 atcgacccga atagcgaacg tagaattaag cgagtgttat ctttatcgct cggcaagcta
      361 attaggtcgc attgatactc tctttatttt tatacactcg attaaattat gaaagtgagg
      421 cggggcagta tgctcagaaa acttgccggc tacaagtaca tgatagcaac tctcctttat
      481 gcctccctgt ttttcactca gtcgcttacc ctaacctgga cggtccccta catcgtacaa
      541 gctctcatac ttaacacggc cataatgctc ggcatatacg ccttcagggg cttcgacaac
      601 tcgactacca cctctatgaa ctcgtgtttc gtctcttttc tttccggcac gatactgggc
      661 gtcgtcttcg ccatgatccc gatactcttc tttcacgccc gactccccag attctccata
      721 gcgatcacgg ctgctctctc tatcttcata ttcccgttta ttggttgcat actcatgaga
      781 tacaccataa aacatatgcc cccgagaaga tacctcgtga taggaaggga agaagagctc
      841 tcgcccatac tggaggaagt cagaaaggcc tcgatgaaca aaatacagat ctactcctac
      901 ctcaacccct ctccggccac ccttgcggcg gccatagaga tagaaacggc caggccctac
      961 gacgccatac tgatcggcga cccccagctg gccaggggag tggagcatat actcaacgaa
     1021 gcgatcacga aaaacacgga tatagaatac ctccccacac tggtggaaaa cacgctccac
     1081 aggatcccga tcgaaatcat cacgatattc aaagactact accagataat cttctcccag
     1141 gcgagatact ccaggagaat cagggtaatg gacatcctct tctcgggcgc gttgctcgtc
     1201 ctgttctctc ccttcattct cttcagcatc gtatacatac tggcccggga cggcaggccg
     1261 gtcttttaca gccagccgag acacggcttg aacgcgaaga tattcagggt ttacaaattc
     1321 cgcaccatga acgacccgga agaagaagag accaaaccga ccataacaag gagcggcaaa
     1381 ctgcttcgaa tgacccggct aaacgagatc ccgcagcttc tgagcgtctt gaagggcgac
     1441 atgagcctcg tcggcccccg gcccgacata ccctcgacct acgagtactg cacaaaggag
     1501 atccccttct acagatacag aacgaagata atgcccggca tcaccggcca cgcccaggtg
     1561 ctctaccggt acgtggacaa actcgaatac gacaccttcg caagaaggct ttcatacgac
     1621 ctttactacg tcaaaaatat ggatttcagg ctgtacctgt cggtcatgct ccgcacggcc
     1681 gaaaccatgg tctttcgaag gggtgccaag tgatgacgga aatactcgta tccaacgtgg
     1741 aaaaatggct gacgcctcac cttgaaaggg tattggctat caaatacacc gagcaattga
     1801 aacactcgca cttcgacagc ttcgaccctg agaggccgta cattatcggc ttcgaataca
     1861 tcggagagtt cacagacatg ggctctctgg gtgacgtgct ccatctggcg agtcgcaccg
     1921 gacgagtgaa gaggatcata tacttctcct cctacggcgt gtacaacccc gggcgaaaac
     1981 agttcaacga atccgacgtc gtctgcccca tgaacttcgt cggagccaga tcggcaatgc
     2041 tcgaggacat cctcgtctat ctggcgaacc gctactcgct cgacctcacg atcctcaggc
     2101 tcttcaatcc ctacggcccc taccagctct cgccctacgt cgtacccacc gtactcgaga
     2161 ggatcgcgac ctcaggcacc gta
//
LOCUS       DAOOLY010000344         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_63005_length_2178_cov_2.955723, whole genome shotgun sequence.
ACCESSION   DAOOLY010000344 DAOOLY010000000
VERSION     DAOOLY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63005_length_2178_cov_2.955723"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLD15_11965"
     CDS             complement(<1..907)
                     /locus_tag="PLD15_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64675.1"
                     /translation="MKKLLVLIITALIGLTALGVYMKAAVLGEVRSLNPYFINSSAER
                     QLIGYLYETLMTTSEGKAIGMLADSWEVNLEELYIVFHLKDRLFHDGSPVTAEDVAYS
                     FNITVQKRLPMGPLLAWFSSAEVLDEKTVKLNFRVLNSFVVSSIPMAIPIIPESVWKN
                     IANPMEFSNLDNPMGTGSLKFKEITPQSVTFSSNGNHPDSPQYLEGIVFNLVQDETMG
                     FLGLIRGDYDYIYWDLDPSLATQMLKDPDRYKDLGLAVTNGDGVVSLLFNHRTLPGSD
                     LNFRKAVQMAIDYAQIVDRVYLGFAD"
     gene            complement(1022..1807)
                     /locus_tag="PLD15_11970"
     CDS             complement(1022..1807)
                     /locus_tag="PLD15_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPB64676.1"
                     /translation="MEQPKSRYVKVLDKKIHYLEVGKPSGIPVVFVHGNFASGKWFEP
                     AMEMLPESYHAFALDLPNFGRSDRTEEVSIDNYGKHVVDFIKQLELEGVILVGHSLGG
                     AVVQNVMVRRPDLLSRVVLIDPAPPDGLKTPPEVYPFLDMYRNNRELLKKALIGTMPT
                     RPVDEFTDLLVDEALLMDPRCFTENARALEKYDYTQQLKGCEIPVLVIVGRKDMLITE
                     EMARGFERIMHTVHVRVLEEYGHSINVENPGLFVDMLLEFTSK"
BASE COUNT          566 a          539 c          494 g          579 t
ORIGIN      
        1 catcggcaaa accaaggtaa accctgtcaa ctatctgggc gtagtcaata gccatttgta
       61 cagcttttct gaaattcaaa tccgatccgg gtagagttct gtggttgaac agcaaagaca
      121 caacgccgtc tccgttcgtg actgccaacc caaggtcttt gtatctgtcg ggatccttga
      181 gcatctgtgt cgccagagaa gggtcgaggt cccagtaaat gtaatcgtag tcgcctctga
      241 taaggccgag gaagcccata gtttcgtcct gaaccagatt gaagactatc ccttcgagat
      301 actgcggaga atctgggtgg ttaccgtttg aggagaacgt taccgactgc ggcgttattt
      361 ctttgaattt cagggaaccg gtacccattg gattatccag attggagaac tccatcggat
      421 tggcgatgtt cttccataca ctttcgggga tgatgggaat ggccatcggg atggaactca
      481 ctacaaagga gttgagaacc ctgaagttca gtttaacagt cttttcatct agaacctccg
      541 cgctactgaa ccaggcaagt agcggaccca tgggtaacct cttttgaacg gttatgttga
      601 aggagtaagc aacgtcttcg gccgtcaccg ggcttccatc gtggaaaagc ctgtctttga
      661 gatgaaaaac gatgtacagc tcttcaaggt tgacttccca agagtctgca agcataccaa
      721 tagcctttcc ttcactggta gtcatcaacg tttcgtaaag gtagcctatc aactgtctct
      781 cggcacttga attgatgaag taaggattca gagatctcac ttcacctaga actgctgctt
      841 tcatgtaaac acccaatgca gtaaggccga tcagagcggt gataatcaac acgagtagct
      901 tcttcattga acatccctcc tcaataaccg gaaaatagcc gtttttcacg gcatcgatag
      961 aaggatttta gcacagctga gtttgccggc ccaaatgcaa aaaaaggtcc cttgcacgta
     1021 tctatttaga ggtaaattcc agtagcatat ctacaaaaag cccgggattc tctacgttta
     1081 tactgtggcc gtactcctcg agcactctca catgtactgt gtgcattatt ctctcgaaac
     1141 ctctggccat ctcctcggtg atcaacatgt cttttcttcc aactataacc agaacgggga
     1201 tttcacagcc cttcaactgc tgggtataat cgtacttctc aagcgcgcgg gcattttcag
     1261 tgaagcatct tggatccatt agaagcgcct catcgaccag caaatcggtg aattcatcta
     1321 ccggtctagt tggcatggta cctatgagag ccttcttcag aagttctctg ttgttcctgt
     1381 acatatctag aaagggatag acttcgggcg gtgttttgag accgtccggc ggggcagggt
     1441 ctatcagcac aactctgctg agaagatcgg gtctgcgtac cataacattc tggacaacgg
     1501 cacctccgag ggaatgcccg accagtatca ctccttcgag ttcgagttgt ttgataaaat
     1561 cgactacgtg ctttccgtaa ttgtctatac tcacctcttc ggttctatca gaccttccga
     1621 agttcggcag atccagcgca aaagcgtgat aactctctgg aagcatttcc atggccggtt
     1681 cgaaccactt tcccgatgcg aagtttccgt gcacaaaaac cacaggaata ccactgggct
     1741 tgccgacctc gaggtaatgg atctttttgt caagtacctt aacatatctg cttttaggct
     1801 gttccataga gtcttgatac ctcccatgaa gagaatgtgt atctccatcg ctgcccgaaa
     1861 ggtcgtctct ttctatagaa tgaatctgcc cataagatag atcaaatggg acgtcccgta
     1921 ccaatctggg cgattccgga atacgattat tatagcagag acaccagccg atactgaaag
     1981 cccctccatc cctgtctaga cattcctttg cgatgttttg gttgggatag agtgaaaacc
     2041 tgtgtggcgc actagatgaa tcataagcgc agatcaggct gggcaggaga agaaagacag
     2101 tcaaacagac aatctccatg aatctcgatc gtccactcag agttttgtcc agccattttt
     2161 tcataaccac agcatctc
//
LOCUS       DAOOLY010000345         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_63394_length_2168_cov_4.379555, whole genome shotgun sequence.
ACCESSION   DAOOLY010000345 DAOOLY010000000
VERSION     DAOOLY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63394_length_2168_cov_4.379555"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLD15_11975"
     CDS             <1..614
                     /locus_tag="PLD15_11975"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPB64677.1"
                     /translation="LSLKESGILFTGHTEILAELTGTRDPLTMFEIYGEMRIFFLSRH
                     VSLRQACDMVKKERVLDYIMRCTEALKQLQLKDSRPFAVAGLNPHCGENGLFGREEID
                     EIIPAIEEARKAGIKVAGPFGADSIFAMARKGEFSAVLSMYHDQGHIASKTLDFARTV
                     SVTLGMPLLRTSVDHGTAFDIAGKGLADPASLIEAVKLAAIYA"
     gene            882..2123
                     /locus_tag="PLD15_11980"
     CDS             882..2123
                     /locus_tag="PLD15_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPB64678.1"
                     /translation="MKKKILLVAVMALLVVSAFAGKVTIWSWRTQDAEVWQKIEALLK
                     SRGQDITIEFTAYAPTEYDSKVNLALQTGTGPDIVYSRRLPGGRTQVLIENGLYLPIS
                     DHVDLTYFPQASLNSVTWENKVYGVPFAVQVVGIYYNKDYYDQFDLKEPTTWDELVAN
                     AEVLKKNGIDPFFVSGKEAWTLTMQHAMVGVSILGPEWIKDLTEGKTNFLDPKFTDVN
                     KRLNDLKIYYQNGFMGNSSVDQDASFAFGQTAMVFYGVWGYQTWKQLNPDINVGYFMV
                     PPITTDQKPYAYTYLDGAIALTSNVKNMEDSIEILKFCATPEFGTIFAGVTYNIPAVT
                     GSTLPPDPVLQEALEVYSNHASPYVYWVGSVFTTQKPSLYDDVLSPGMQEMYAGKLTP
                     EGLSKMAQDAISQWYPPLMNK"
BASE COUNT          568 a          554 c          536 g          510 t
ORIGIN      
        1 aactatcctt gaaagaatcc ggtatactct tcaccggcca cacggaaatt ctggccgaac
       61 tcaccggcac ccgcgacccg ttgaccatgt tcgaaatcta cggagagatg aggattttct
      121 ttctttccag gcatgtatcg cttcggcagg cctgcgatat ggtgaaaaaa gagagggtgc
      181 tggattatat aatgcgctgc acggaagcct taaaacaact gcagttgaaa gactccagac
      241 cttttgcggt tgccggcctc aatccgcatt gcggagagaa cggtctcttc ggcagagaag
      301 agatcgatga gataataccc gccatcgaag aagccagaaa ggcggggatc aaggtcgcag
      361 gtcccttcgg ggccgattcg atcttcgcca tggcccgcaa gggagagttc tcggccgtac
      421 tgtccatgta tcacgatcag ggccatattg cgtccaagac actggatttc gccagaaccg
      481 tttcggtgac tcttggaatg cctctattaa gaacctctgt cgatcacggc actgccttcg
      541 acatagccgg taaaggttta gcagaccccg ccagcttgat cgaagcggtg aaactcgccg
      601 ctatatatgc ttgatttcgc ccaattagcc actgatagac catagacggc tataaatggc
      661 gagaatcgat tctgttcgct atgttttaag acttcgtttt tgtatggccc gtattgtata
      721 atcattctga caatactaga cttttaagag gtggtgtttc ggtagtcagc tgttgtaagg
      781 gcattcagga ctacatcttt tcgataaatg ttgtatagat gttattgagc atagaataag
      841 tgaaccatcc gttctcaact ttaagtgtta ggaggggaaa aatgaaaaag aaaatcttgc
      901 tagttgctgt gatggcactg cttgtggtga gtgcctttgc cggcaaagtc accatctgga
      961 gctggcgaac tcaggatgcc gaggtctggc agaagatcga agcgctcctg aaaagccggg
     1021 gccaagatat aacgatcgag ttcaccgctt acgcgccaac cgaatacgac tcgaaagtca
     1081 acctcgctct tcaaacagga acaggaccgg acatagttta ctccagaagg ctccccggcg
     1141 gaagaacaca ggtgctgata gaaaacggtc tctacctgcc gatcagcgat catgtcgatc
     1201 tgacgtattt cccccaggcc tctctgaatt ctgtgacctg ggaaaacaaa gtgtacggag
     1261 ttccctttgc ggtacaggta gtcggaatct actacaacaa agattactac gatcaattcg
     1321 atctaaaaga accgacaaca tgggacgaac tggtggccaa cgccgaagtt ctgaagaaga
     1381 acggcatcga tcccttcttc gtatcgggta aggaagcctg gacactcacg atgcagcacg
     1441 cgatggtcgg agtcagcatt ctcggaccgg aatggataaa ggatctcaca gaaggcaaga
     1501 ccaacttcct cgatccgaaa ttcaccgacg tcaacaagag actcaacgat ctcaagatct
     1561 actaccagaa cggattcatg ggcaactctt ctgtcgatca ggacgcttct ttcgccttcg
     1621 gacagaccgc gatggttttc tacggtgtat ggggctacca gacatggaag cagctcaacc
     1681 cggatatcaa cgtgggttac ttcatggttc ctccgataac cacagatcag aaaccatacg
     1741 cttacaccta tctggacgga gccatagcac tgacgtccaa cgtaaagaac atggaagatt
     1801 cgatcgaaat cctgaaattc tgtgcgaccc ctgagttcgg aaccatcttc gccggggtaa
     1861 cgtacaacat ccccgcggtg accggttcga ctcttccgcc tgatccagtt cttcaagagg
     1921 ctctggaagt ttactccaac catgcctctc catacgttta ctgggtcggc tcggtcttca
     1981 ccactcagaa gccttctctg tacgacgatg ttctgagccc gggtatgcag gaaatgtacg
     2041 ccgggaagct cactccggaa ggactatcca agatggctca ggatgcgatt tctcagtggt
     2101 acccaccgct aatgaataag taaacctttg attttcactc ttccccgcct ccgcgcgggg
     2161 gagagttc
//
LOCUS       DAOOLY010000346         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_63646_length_2163_cov_3.649905, whole genome shotgun sequence.
ACCESSION   DAOOLY010000346 DAOOLY010000000
VERSION     DAOOLY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63646_length_2163_cov_3.649905"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="ffh"
                     /locus_tag="PLD15_11985"
     CDS             <1..1004
                     /gene="ffh"
                     /locus_tag="PLD15_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPB64679.1"
                     /translation="GLQGSGKTTTTAKLATLLRKEGKKPLLVAADVYRPAAIDQLIQL
                     GKQIDIPVFTGDRRNPVSIVREAMQQAIKNINDVVILDTAGRLHIDDTMMGELKEIAT
                     LVKPDEILMVVDAMTGQDAVNSAKAFNEVLELSGFVVTKLDGDARGGVILSIRHVTGK
                     PVKFIGISEKPDGLEVFYPDRVAGRILGLGDVLSLIDKIQANVDEEKAREMEEKFLKN
                     KFDLEDFLDQLREIKKMGSIADILEMIPGAPKDVDLAGSEKSMKRTEAIIHSMTLKER
                     RNPKLLNYSRKQRVARGSGTTIQDVNKLLKSYDQMKDMMKQFNRKGKLRKGIKGFPF"
     gene            1039..1326
                     /gene="rpsP"
                     /locus_tag="PLD15_11990"
     CDS             1039..1326
                     /gene="rpsP"
                     /locus_tag="PLD15_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPB64680.1"
                     /translation="MVKIRLTRMGRRNRPFYRLVVVDSQKRRDGAYIDSLGFYDPIRD
                     PAQTNVDVEKAVQWIMKGAQPTETARSILSKFGVMKKVNELKYVKSGETKE"
     gene            1326..1559
                     /locus_tag="PLD15_11995"
     CDS             1326..1559
                     /locus_tag="PLD15_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPB64681.1"
                     /translation="MKEVLEHILKGIVREPDLIKVTDTTDENGNRVFEIRASESDVGQ
                     IIGKDGRTIKSINVLMNALAGSSKESFILKVVR"
     gene            1556..2104
                     /gene="rimM"
                     /locus_tag="PLD15_12000"
     CDS             1556..2104
                     /gene="rimM"
                     /locus_tag="PLD15_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491755.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPB64682.1"
                     /translation="MKSGSFDNFEGMIPIGRIVKPHGLRGEVKAKIAIDNDEVFRKFK
                     NAVLSANETSRVKVRIDGYRKAVKGWILHFEGFETLSQVERLSGYYIYVERDYLPELA
                     EGEYYFFQVLGCQVRNESGDFVGTVGDIIETGSNDVMVIRRQDGFNVHEELIPLIKDY
                     IVSMDFERKIVIARSLDYEEVR"
BASE COUNT          678 a          461 c          594 g          430 t
ORIGIN      
        1 tgggtctcca gggaagcggt aaaacgacca ccacggcgaa actggctact ctccttcgaa
       61 aagaaggcaa gaaaccgctt ctggtggctg ccgacgttta taggccggcg gccatcgacc
      121 agttgatcca gcttggaaaa cagatcgata ttccggtgtt caccggcgat cgcaggaacc
      181 cggtgtcgat cgtcagagaa gctatgcagc aggcgataaa aaatatcaac gatgtcgtga
      241 tactcgatac ggcaggcagg ctacacatag acgatacgat gatgggtgag ctgaaggaaa
      301 tagccacatt ggtgaaaccc gacgaaatac taatggtggt tgacgcgatg accggccagg
      361 atgccgtcaa ttctgcgaag gccttcaacg aagtgctgga actgtccggt ttcgttgtca
      421 ccaagctgga cggcgacgcc aggggcggtg tgattctctc gataagacac gtaacaggaa
      481 aacccgttaa gttcatcggg atctcggaga agcccgacgg cctcgaggtg ttctatccag
      541 acagggtcgc cggaaggata ctcggcctgg gcgatgttct atctctgata gacaagatac
      601 aggccaacgt cgatgaagaa aaagctagag aaatggagga gaagttcctc aaaaacaagt
      661 tcgacctcga ggactttctg gaccagctga gagagataaa gaagatgggt tcaatagccg
      721 atatcctcga aatgataccg ggagcgccaa aagatgtgga tctggccggc agcgaaaaaa
      781 gcatgaaaag aaccgaggcc ataatccatt cgatgactct caaggagagg agaaacccga
      841 aactacttaa ctattctaga aagcaacgcg ttgccagagg cagcggcaca accattcagg
      901 acgtgaacaa acttctcaaa tcttacgacc agatgaaaga catgatgaaa cagttcaaca
      961 ggaagggaaa actcagaaaa ggaatcaaag gatttccatt ttaaatagtg aaaacaactc
     1021 aggaggtgca ggcaaaacat ggtaaagata agactcacca gaatgggcag gagaaacagg
     1081 cccttctaca ggctagtggt agtggattcg cagaagagac gcgacggcgc ttatatagac
     1141 tcgctggggt tttacgaccc gataagagat ccggcgcaga ctaacgtcga tgttgaaaag
     1201 gccgtccagt ggatcatgaa gggcgcacaa cccacagaaa cggcgagatc tattctctca
     1261 aagttcggtg taatgaagaa agtcaacgaa ctcaagtacg tgaagagcgg agaaaccaaa
     1321 gagtgatgaa agaagttctt gaacacattc taaagggcat agtcagggaa ccagacctga
     1381 taaaggttac cgatacgacc gacgaaaacg gtaacagggt ctttgaaata agggcctccg
     1441 aaagcgacgt gggccagata attggaaaag atggacgaac cataaagtct ataaacgttt
     1501 tgatgaacgc ccttgccggt tcctctaaag agagcttcat cctaaaggtg gttagatgaa
     1561 aagcggctcc ttcgacaatt ttgaaggcat gatacccatc ggtcgcatag ttaaacccca
     1621 cggactgagg ggagaggtaa aggccaagat agccatcgat aacgatgagg tcttcaggaa
     1681 gtttaagaac gcggtccttt cagcaaacga aacatcacgc gtgaaagtca gaatagatgg
     1741 ttacagaaag gcagtaaagg gatggattct tcacttcgag ggattcgaaa ctctctctca
     1801 ggtggaacgc ctttccggat attacatcta cgtagaaaga gactatctgc ccgaactggc
     1861 cgagggtgag tattatttct tccaggttct aggttgccag gttcggaatg agagcggaga
     1921 tttcgttgga actgtggggg atataatcga aacgggctcg aacgacgtta tggttatcag
     1981 aagacaggat ggtttcaacg tccatgagga actgattcct ctgataaagg actacatcgt
     2041 ttcgatggat ttcgagagaa agatagtgat cgccagatcg ctcgattatg aagaggtgag
     2101 atagagcggt caacgacccc cggcaaaagc cgggagctag tagctcaggt tgactagcct
     2161 aag
//
LOCUS       DAOOLY010000347         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_65262_length_2126_cov_3.186866, whole genome shotgun sequence.
ACCESSION   DAOOLY010000347 DAOOLY010000000
VERSION     DAOOLY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65262_length_2126_cov_3.186866"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..769)
                     /gene="hpt"
                     /locus_tag="PLD15_12005"
     CDS             complement(233..769)
                     /gene="hpt"
                     /locus_tag="PLD15_12005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPB64683.1"
                     /translation="MHFDPSKIEVLYSEKDLERIVERLASEINAYYTPITDEITAVCI
                     LKGSVHFYSDLLKRINLKVRYNFVHVSSYSGSSTTGRIRVKTWVDESVTDKYVLLVED
                     IVDTGGTLRYIINYIWKQKPAGVKLVSLFEKTIHEHGVTIDFTGDRIGDLFVVGYGLD
                     FDEVYRNLPYVGYLKSDS"
     gene            891..1862
                     /gene="trmB"
                     /locus_tag="PLD15_12010"
     CDS             891..1862
                     /gene="trmB"
                     /locus_tag="PLD15_12010"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPB64684.1"
                     /translation="MKNVHHGALMERKVDHFLGNFIDATEYELPLKIVELFHRAGPNF
                     LEIGFGGGEFLIQMAQNKPEWNFVGLELSMVSCQKLLKSVLRKGLKNLRVLLIDAQFA
                     LDRVLPSDSFDGVYMNFPCPWPKKRHSGRRLNGREFMEKVSRVLKPGGFFQLYTDSED
                     FALGMVSSLESTGLYNIRAMEKNPSEGVNTRYEAKWLSAGRQIFRLYSTLESSTARRD
                     VLKEEEMPHKWLSGEIDMDRLEEIYNVPIIEGESLVKFLRVFKSMGEESYLVETITVD
                     NDYSQRFYINLVRKEAGWLVQLDSQAKPFRTPAVKLAVNRLASIVQQ"
     gene            complement(1915..>2126)
                     /locus_tag="PLD15_12015"
     CDS             complement(1915..>2126)
                     /locus_tag="PLD15_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPB64685.1"
                     /translation="AAGAIEAVATIMAMNNSLVPPTINYETPDPQCDLNYTPNKAFAR
                     EIQFAMSNSLGFGGHNATLLFKRIS"
BASE COUNT          580 a          500 c          501 g          545 t
ORIGIN      
        1 tccgtcttca gagagtaacc gttcatctca agatacaact cgcaactctt ctcaacgctg
       61 aagtccagca actttctggc agactcgagg acgcctctcc tgacagaagc ctctaccatc
      121 agcctgcaag tctcacgaaa cgaacgaaca ctcttcagaa cgaacgaaac ggatatcgcg
      181 caggcacaca aaagcaaaat cgaaataacc agtaccgtaa tgcttcccct gttcacgagt
      241 ccgacttcag atagcccaca tacggcaaat ttcgatacac ttcatcgaag tccaatccat
      301 agccaactac gaacagatct cctatcctgt cgccggtgaa gtctatggta acaccgtgct
      361 catgaatcgt cttttcgaag agcgatacca gcttcacgcc ggcgggtttc tgcttccata
      421 tgtaatttat tatgtatcga agcgttccac cggtatcgac gatgtcttcc accagcagga
      481 cgtatttatc ggtaaccgac tcgtccaccc aggtcttcac ccttatccga ccagtagttg
      541 aacttcccga ataactggaa acgtgaacaa aattgtatct cacctttaga ttgattctct
      601 tgagcaaatc actgtaaaag tgtaccgatc ctttcaaaat acagacggcc gtgatttcat
      661 cggtgatagg cgtataataa gcatttattt cgctcgcgag ccgttctaca attctctcga
      721 gatctttttc cgaatatagt acttcgattt tcgaaggatc aaagtgcatc atattcacct
      781 ccagagagtc aggataatta tatcattaac gaacgcgacg catagtcccg tacgcggttt
      841 agagtgttaa atactggaaa tggaaacgta tttcggatac aatcttctag atgaaaaacg
      901 tacatcacgg agcgctaatg gagagaaaag tggatcattt tttaggaaat ttcatcgatg
      961 cgaccgaata tgagctgcct ttgaagatcg tagaactctt ccatcgtgcc ggtccgaact
     1021 tcctcgaaat aggcttcgga ggtggcgagt tccttattca gatggctcag aacaagccag
     1081 aatggaattt tgttgggctt gaactctcga tggttagctg ccagaaactc cttaaatccg
     1141 tcctgagaaa gggtttgaaa aaccttcgtg ttctcctgat agatgcgcag ttcgcgctgg
     1201 acagagtcct tccgtcggac tctttcgatg gagtttatat gaatttcccc tgcccctggc
     1261 ccaaaaagag acactcgggt agacgcttga acggcagaga gttcatggag aaggtctcga
     1321 gggtgttgaa accaggcggt ttttttcaac tctacaccga ttcggaagac ttcgcgcttg
     1381 ggatggtctc gagtctggaa tcgaccggat tatacaatat aagagctatg gaaaagaatc
     1441 cctcagaggg ggtgaacaca aggtatgaag ccaagtggct atcggcagga aggcagattt
     1501 tcaggcttta cagcactctc gagagcagta ctgccaggcg cgatgttttg aaggaggaag
     1561 agatgcctca caagtggttg agcggggaaa tcgatatgga caggctggaa gagatttaca
     1621 atgttccgat aatcgagggc gaatctctgg tgaagttttt gagggtattc aaatcaatgg
     1681 gagaagaaag ttacctggtg gaaactataa ccgttgacaa cgattacagc cagaggtttt
     1741 acatcaatct ggtacgtaag gaagcgggct ggctggttca gctcgatagt caggccaaac
     1801 ccttcagaac gcctgccgtg aaactggctg tgaatagact tgcatcgatt gtccaacaat
     1861 aaaaaacggg gcgcttctgc gccccttcca gagtgtggca aatttctcct cagcttatga
     1921 aattctcttg aacagaagcg tcgcattgtg tccgccaaaa cccagtgagt tactcatggc
     1981 gaattgtatt tctctggcga aggctttgtt tggagtataa ttcagatcgc actgcggatc
     2041 gggagtctcg taatttatcg tcggaggtac cagagagttg ttcatcgcca ttatcgtggc
     2101 taccgcttct atcgctccgg ccgcac
//
LOCUS       DAOOLY010000348         2119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_65584_length_2119_cov_3.488372, whole genome shotgun sequence.
ACCESSION   DAOOLY010000348 DAOOLY010000000
VERSION     DAOOLY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65584_length_2119_cov_3.488372"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLD15_12020"
     CDS             <1..349
                     /locus_tag="PLD15_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB64686.1"
                     /translation="FGFRTKLELKDMTYDSPRIMNEKGVLAAFMCDHPVIPLESTNIQ
                     LGVALRYGSKEEDLLKMVTINPAKILEIDDRVGSLEVGKDADIAVWSGHPFQFSSRVE
                     MVFIEGEEVYNSK"
     gene            568..1215
                     /locus_tag="PLD15_12025"
     CDS             568..1215
                     /locus_tag="PLD15_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPB64687.1"
                     /translation="MDLSEYSFFNPSPNFREMSILKALTESSSVSQERLARLAGIVPS
                     MVNKYIKDFEDSGLIQKEGENRRNMKYVLTEAGKFRLQYLTILYLKEAAKLYTESREI
                     FGEVLDVLKESEHESFYLYGAGIIGGILADILRTEGMRIIGFLDDSPAKQNGTFHDLH
                     VFSPDEAECKDETAVIVASFRHADNIIKKARESGMKNVMVFTISKMGKVELEQVE"
     gene            1387..>2119
                     /locus_tag="PLD15_12030"
     CDS             1387..>2119
                     /locus_tag="PLD15_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB64688.1"
                     /translation="MKIPLFDLTRQYRGFREEVLEKIDEALLNGRVILGEAVKELEKS
                     VAELIGVKHAIGVANGSDALLIAVAALKIGPGDYVITTPYTFFATVSCITRNGATPLF
                     ADIDPVTYNIDLDEVEKILRTHPEREKIKAIIPVHLFGRAIDLERLEKIREKYGVRII
                     EDCAQSIGASWTYEDGTVKTTGSIGELSTLSFFPTKNLGAYGDAGMIVTDDDELAAFC
                     RSYRVHGSPVKYVHDMIGINSRLDEL"
BASE COUNT          636 a          465 c          521 g          497 t
ORIGIN      
        1 cttcggcttc agaacaaaac tagaactgaa agacatgacg tacgattcac cgagaataat
       61 gaatgaaaaa ggcgttctgg ccgccttcat gtgcgaccat cctgtgatcc cgctcgagag
      121 tacgaacata cagctcggtg ttgccctcag atacggttca aaagaggaag atctgttgaa
      181 aatggtgacg ataaatcctg caaagatact cgaaatcgac gacagggttg gctcgctcga
      241 ggtaggaaaa gatgccgata tagcagtgtg gtcgggacat cctttccagt tctcttcaag
      301 agttgaaatg gtatttatag aaggagaaga ggtatacaat agcaaataaa tccgtttgtg
      361 tatagaccgg atttgacatt caaaccatat taggaaattc ttattttaaa ggcagatcac
      421 agccgctggc tcttgtcagc ggtatgcctt tgaaatttaa ttctcactca caatttgttt
      481 tatccagtat gtgccttctt ttgcttgact actaaattga atctctgata taattctctc
      541 agtgaatgaa tgaaattagg aggtcttatg gatctttccg aatattcgtt tttcaatccc
      601 tctccaaatt tcagggagat gagcatactc aaggctctca cggaaagctc ttcagtttcc
      661 caggaaaggc ttgccagact ggcaggcata gttcccagca tggttaacaa atacataaag
      721 gatttcgaag attcggggct aatccagaag gagggcgaga accggagaaa catgaaatac
      781 gtactgacgg aggccgggaa gttcagactc cagtatctca ctatccttta cctcaaagaa
      841 gcggcaaaac tgtacacaga atcgagagag atcttcggag aggttctgga cgtgctcaaa
      901 gaaagcgaac acgaaagctt ctacctgtac ggagccggaa taatcggcgg cattctcgcg
      961 gatattttga gaaccgaggg aatgagaata atcggctttc tggacgactc gccggccaaa
     1021 cagaatggaa cctttcacga tcttcatgtc ttctcaccgg atgaagccga atgtaaagat
     1081 gaaaccgcgg tcatcgtggc atcgttcagg catgcagata acataatcaa aaaggccaga
     1141 gagagcggaa tgaaaaacgt gatggtcttc accatatcga aaatgggaaa agtggaactt
     1201 gagcaagttg agtaatgaga aagactattt tcgagagggc gagatgacga aagaatggtc
     1261 acggaggcag agctggccag aaaaccgcag ttatgaatct gtggttatct tctccccgtc
     1321 cgtttgcaat ctcggctcca tcttctccaa atacagatgt cctctcgaca ggaacatgga
     1381 ggttttatga aaataccatt attcgattta accaggcagt acaggggttt cagagaagag
     1441 gttcttgaga agatagacga agccttgctc aacgggagag tgatcctggg agaggcagtg
     1501 aaggaacttg agaaaagcgt tgccgagctc atcggggtta aacacgcgat aggagtcgcc
     1561 aacggatccg acgcccttct catagccgtt gccgctctga agataggacc aggagattac
     1621 gtgatcacca ccccctacac tttcttcgcc acggtcagct gcattactag aaacggagcc
     1681 actcctctct ttgccgacat tgatccggtc acttacaaca ttgatttgga cgaagttgaa
     1741 aaaatactga gaacccaccc cgaaagagaa aagatcaaag cgataatacc ggttcacctc
     1801 ttcggacggg caatcgatct cgaaagactc gaaaagataa gagagaaata tggtgtcaga
     1861 ataatcgagg attgtgccca atccataggc gccagctgga cttatgaaga cgggactgtg
     1921 aagacgacgg gttcgatcgg agagctatcg accctttctt tctttcccac caaaaacctg
     1981 ggcgcctacg gagatgcggg aatgatcgtc acagacgacg atgaacttgc ggcattttgc
     2041 agaagctaca gagtccacgg ctcgcccgtg aagtacgttc acgacatgat aggtatcaac
     2101 tctagactcg atgagcttc
//
LOCUS       DAOOLY010000349         2118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_65657_length_2118_cov_2.254968, whole genome shotgun sequence.
ACCESSION   DAOOLY010000349 DAOOLY010000000
VERSION     DAOOLY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65657_length_2118_cov_2.254968"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLD15_12035"
     CDS             complement(<1..860)
                     /locus_tag="PLD15_12035"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPB64689.1"
                     /translation="MKVAFRTFGCQMNISDTEAMMGILRRAGHEIIDSEEAADAVIVN
                     TCAVREKSEDKLYGKLGQLKALKKSNDRLVVGVCGCVAEKNSTELLTHREVDFVFGTR
                     AISRIDEFLSRAAGGERFIEMGDYIDELDANSPRLRTSKHHAWITIIYGCDKFCSYCI
                     VPYTRGREKSRNMMDILNEVRQLADSGYREITFLGQNVDSYGKDLKDGSSLAALLRET
                     LKIEGIERIWFLTSYPRDFSDDLINVIASSEKISRSIHLPVQSGSDRILKAMNRGYTR
                     EYFLDLIRRV"
     gene            1012..1791
                     /locus_tag="PLD15_12040"
     CDS             1012..1791
                     /locus_tag="PLD15_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB64690.1"
                     /translation="MFAKAYKTASSFTKPVVVLTRREDARVGASCGVFIILNREGWII
                     TVAHLWQSYFLFKNQQANPSSNGTQIITNHSFWWGKDGVTLKDIHPFPDYDLVIGRLE
                     PFDPSEVSEYPSFVDSGELRIGTSLCRVGYPFSRIKASFDEGSSRFKMDPRSLPALFP
                     IEGIYTRTVVAPAFKEAKKIAKFIETSSPGLLGQSGGPILDVTGKVWAVQSRTVHLPL
                     GFSPKVRKDGREIEENQFLNVGWGVHVEMIVDAMKSSGIAL"
     gene            1922..>2118
                     /locus_tag="PLD15_12045"
     CDS             1922..>2118
                     /locus_tag="PLD15_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64691.1"
                     /translation="MKKILFVLLLCLAFLAIITTQGCFLFRVIKLDIATKSISLSIGE
                     SETIQLTVKPVDTTLKFTSKNT"
BASE COUNT          560 a          518 c          468 g          572 t
ORIGIN      
        1 ctgacgcgtc tgataagatc cagaaaatac tccctcgtat aacccctgtt catggctttc
       61 aggatcctgt cgctccccga ttgaaccggt agatgaatcg agcgcgaaat tttttcggaa
      121 gaggctatca cgtttatcaa atcgtcgctg aagtcacgcg gatacgatgt gagaaaccag
      181 attctctcta tcccttctat ctttagagtc tctctcaaca gagcggcgag cgacgagccg
      241 tctttgaggt ctttcccgta agaatccacg ttctgaccca ggaaagttat ctccctgtag
      301 ccactgtcgg ccagttgcct gacctcgttc aggatgtcca tcatattccg gctcttttct
      361 cttcccctcg tatatggaac tatacagtaa gagcaaaact tatcgcaacc gtatatgata
      421 gtgatccacg cgtgatgctt gctggtacgt aatcttgggc tgttcgcgtc gagttcgtct
      481 atataatcgc ccatctctat gaaacgttcg ccgccggcgg ctcttgacag aaactcgtct
      541 atccgcgaaa tcgccctcgt tccaaaaacg aaatcaacct ccctgtgcgt gaggagttcg
      601 gtcgaattct tttcggcgac acagccacaa actcctacaa cgagtctatc gttgctcttc
      661 ttgagggctt tgagctgacc gagttttccg tacaacttgt cttccgattt ctcccgcacc
      721 gcacaggtat tgactataac cgcatcggcg gcttcttcac tgtctataat ctcgtgtccg
      781 gcacgacgga gaattcccat catcgcttct gtatcgctta tgttcatctg acacccgaag
      841 gttctgaacg ctactttcaa aaaaccacct ctaaaccgat atccattctc aggtaaattc
      901 tacatcattg accgatttgg ataaaccacg ttatgttacc attggaatga gcggtgttcg
      961 tttctttttt ttgcgtggct cgttcaacag cgatatttcc ggaggtgtca gatgtttgca
     1021 aaggcttata aaacagcgag ttccttcaca aaacccgttg tagttcttac cagaagagaa
     1081 gacgccagag tcggcgcctc ctgtggagtc ttcatcatcc taaaccggga aggctggata
     1141 atcaccgttg cccatctatg gcagtcctac ttcctcttca aaaatcaaca ggcaaatccc
     1201 tcgagcaacg ggacacagat tataaccaac cattctttct ggtggggaaa ggacggggtg
     1261 actttgaaag atatacatcc cttccccgat tacgatctgg tgataggaag attggagccc
     1321 ttcgatccct ccgaagtgag tgaataccct tcattcgtag attcaggaga gttgcgaatc
     1381 ggaaccagtc tttgccgtgt tggatacccg ttctccagaa taaaggcttc ctttgacgaa
     1441 ggcagttcga gattcaaaat ggatcccaga agtctaccgg cgctgtttcc catcgaaggc
     1501 atatacacca ggacggtggt ggctccggct ttcaaagaag cgaagaaaat tgcgaaattc
     1561 atagagactt catctccggg actgctcggt caaagtggtg gacctatact ggatgtaaca
     1621 ggaaaagtct gggccgtgca gagtcgcacc gttcacctgc cgctcggatt cagtccaaaa
     1681 gtgcgaaaag acgggagaga aatcgaagaa aaccagttcc ttaatgttgg ctggggagta
     1741 catgtagaga tgatcgtaga tgcaatgaaa tcatcgggaa ttgctctata atatacggga
     1801 tttattatga aaagactctg gaataccaaa aagaattata gatcaagata gctttgtggt
     1861 aaaatcataa gtaaatatac agtttacaat tctaatcttt ttcttcaagg agtagattac
     1921 tgtgaagaag atcttatttg tattactact gtgcctggct tttctcgcca tcataacgac
     1981 tcaaggctgt ttcctcttta gggtgatcaa gctggacatc gcaacgaaaa gtatctccct
     2041 ctccatagga gagtcggaaa cgatccagtt aaccgtgaag cccgtagata caacgttgaa
     2101 attcacgtcg aaaaatac
//
LOCUS       DAOOLY010000350         2111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_65950_length_2111_cov_3.623541, whole genome shotgun sequence.
ACCESSION   DAOOLY010000350 DAOOLY010000000
VERSION     DAOOLY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65950_length_2111_cov_3.623541"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(712..1503)
                     /gene="rpsB"
                     /locus_tag="PLD15_12050"
     CDS             complement(712..1503)
                     /gene="rpsB"
                     /locus_tag="PLD15_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPB64692.1"
                     /translation="MAVVSMKQLLEAGVHFGHRTRRWNPKMKPYIYGERKGIYIVDLQ
                     KTLKLIEEAYEHVRNRAQEGATFLFVGTKRQAQQIVEEEAKRSGSFYVNSRWLGGLLT
                     NFSTIKRRIEALKNFEEMETSGKLAALPKKEQSMINKRLEKLRKNLNGVKEMHKLPDV
                     IFIVDPKQEEIAVSEANKLGIKVVGIADTNCDPDVIDFIIPGNDDAIRAIQLIVHTMA
                     DAILEGREGLDASVLASKSSEATTVKGPEELPDIGETEELEETDE"
     gene            complement(1580..1948)
                     /gene="rsfS"
                     /locus_tag="PLD15_12055"
     CDS             complement(1580..1948)
                     /gene="rsfS"
                     /locus_tag="PLD15_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPB64693.1"
                     /translation="MDNTVKEITEILDKKQGEEIVILDVSKVSNLSDYFVVATANSDP
                     HMEALREAVLEYLERESVPIVYYDKGRGYDWLVVDGGYFIVHIFSKKGREFYSLEDLW
                     LNAKRYTYRELVKDEKNARQ"
BASE COUNT          489 a          523 c          450 g          649 t
ORIGIN      
        1 gcatacttgc ccgtttcaga ctgcggattg gcagtaacag tgccattctc ggcattgatc
       61 gtcagagtgt aaaacacttc tggtttggga cagccagtaa gcatcaacac aacaatcaaa
      121 atgctgagca atgtggttaa cttcaagagt ttctttgcca ctgcacacac cccctataag
      181 gcgttttggt tgtgaagtat tttgctaaga acatagagac ctgtatttac caaaaaatgc
      241 atcccgtaca agaaaattcg ataatctaat ctaacaagac ttgccttata acatattata
      301 taccacacac aacgcgctaa gcaaaatcac cagaaacaga ccaaaccata ggtaattgta
      361 ccatttccaa taatgccgtg tcaaacgctt ttcatgggtt gatgatgttt ataagcgaaa
      421 tcatacttac ttacaaatca cagcaaataa ccagttttct tccgggagat tgaggcgact
      481 acgttttcaa ctcgcctttc tgtagttgat tatacactat aaaacggctt tcttgtccat
      541 gacttacgac tcttcttcac ttccaaaatt tcttttttgc tgcgaaattc tggaatatca
      601 ttaatgatgt ggagcatcac ttaaaattaa aaaggccact tcccctggag ccaaacttaa
      661 cgggaaaatg gccgtattaa tgaattttaa ttaaacgctg gaccttctag attattcgtc
      721 agtttcttcg agctcttctg tttcgccgat atcggggagt tcctcaggtc ccttaacggt
      781 cgttgcttcg cttgacttag acgcgagcac cgatgcgtca agtccttctc taccttcgag
      841 aatggcatcg gccatagtgt gaactatcag ctggattgcc cttatcgcgt cgtcgttacc
      901 gggaattatg aaatctatga catcgggatc gcagttggta tcggctattc caaccacttt
      961 tattccgagc ttgtttgcct cggaaaccgc tatctcttcc tgcttgggat cgactataaa
     1021 aattacgtcg ggaagcttgt gcatctcttt gacgccgtta aggttctttc tgagcttttc
     1081 cagtcttttg tttatcatgc tctgctcttt ctttggcagg gccgcaagct ttccgctggt
     1141 ctccatttct tcgaagttct tcagtgcttc tattcttctc ttgatggtgc tgaagttggt
     1201 cagcaaacca cctagccagc ggctgttgac atagaaagaa ccgctcctct tggcttcctc
     1261 ttcaactatc tgctgggcct gtctcttggt tccgacgaac aggaatgtgg caccttcctg
     1321 ggcacggttt ctcacgtgct cgtaggcctc ttcgataagc ttgagagtct tttgaagatc
     1381 tacgatgtag attccctttc tctcaccgta gatgtagggt ttcattttcg ggttccatct
     1441 tctcgtcctg tgaccgaagt ggacaccggc ttcgagaagt tgtttcattg atactactgc
     1501 cacgtaaaca cctccgattt ggtttacctc cggcgcgtgc tgtggatcga cctctttcga
     1561 ggcaccggga tccaagcgct tactgccgtg cgtttttctc gtctttgacg agttctctgt
     1621 atgtgtatct ctttgcgttc aaccagagat cttcgaggga atagaattcc cgtccctttt
     1681 tgctgaagat gtgcactatg aagtatcctc cgtcaacgac cagccagtcg tagcctctgc
     1741 ctttatcgta gtacacgatc ggaacgcttt ccctttccag atattcgaga acggcctccc
     1801 tgagagcctc catgtgagga tccgagttgg ccgtagcgac tacgaagtag tcggaaagat
     1861 tggagacttt cgagacatcg aggattacta tctcttctcc ctgtttttta tcgagaattt
     1921 ctgtgatttc ttttactgta ttgtccaaat caaactctcc catttttgtc ggttcaccgc
     1981 tcattctaca gtgacgcttt tggccaggtt cctcggtctg tctggatcgt atcccctcgc
     2041 tacggcaacg tggtaggcga agagctggag gtacggcgac atcaccagcg ggtatagggg
     2101 cgccgaaacc t
//
LOCUS       DAOOLY010000351         2106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_66156_length_2106_cov_3.893710, whole genome shotgun sequence.
ACCESSION   DAOOLY010000351 DAOOLY010000000
VERSION     DAOOLY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66156_length_2106_cov_3.893710"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1016
                     /locus_tag="PLD15_12060"
     CDS             84..1016
                     /locus_tag="PLD15_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64694.1"
                     /translation="MPKLFYRKKLDELEEKARPIHINSYWQLVWVKFRSHKLAMFGAV
                     VLIIVVAFTIFGPLFVEVGYEDMDFSSLFKPPLSQGHPLGTDELGHDVLVRVMYGGRI
                     SLFVGFASSVITTLVGTLIGLLSGFYGGLIDRFLMRFVDVMLSIPMFPILLTLTLVFG
                     SGIMNIILVLTVFGWMGVARLVRGLVLSIRETEYVMSARAMGVRSARIILRHVLPNVI
                     PIVIVSATLNMSYAILSESSLSYLGLGIQPPMPSWGNMLQKAMNYILGTATGANPWWL
                     TFFPGFFIFLTVLSVNFLGDGLRDALDPKFVSES"
     gene            1022..2014
                     /locus_tag="PLD15_12065"
     CDS             1022..2014
                     /locus_tag="PLD15_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64695.1"
                     /translation="MQEILKVENLKITFKTQLGKITPVDGVSFGLSEGETLGIVGESG
                     CGKTITAFSIIKLLPKNAILGDETKISFRGRDIATLEKSDLHKIRGKSISMVFQEPMT
                     SLNPLYTIGWQISEVYKLHEGLDDEEAMDRSIEMLRLVGIPEPAKRVKEFPHQLSGGM
                     RQRVMIAMALACSPALLIADEPTTALDVTIQAQVLELMNDLKNKLDTATIIITHDLGV
                     IAEMCDRVLVMYAGQIVESADVFRIFDNPLHPYTRGLIHSIPRIEVEKRDQKRLNVIT
                     GYVPHPSKFPTGCRFEPRCLMAMEKCKKETPQIYSPEEAHTVRCWLAEGSETNG"
BASE COUNT          514 a          548 c          572 g          472 t
ORIGIN      
        1 actctgattt ctaacctcat tgccgatctg ctctatgtac tggtcgaccc gaggataaga
       61 atgggaagaa agggggcctg aatatgccga agctatttta tcgaaagaaa ctggacgaac
      121 tcgaagaaaa ggcccgcccg atacatataa acagctactg gcagctcgta tgggtcaagt
      181 tcagaagcca taagctggcc atgttcggag cggtcgtgct tataatagtc gtggccttca
      241 ccatcttcgg cccgctattc gtagaggtcg gctacgagga catggatttc tcctccctct
      301 tcaagccgcc tctgagccag ggtcatccgc tgggaacaga cgagcttgga cacgatgttc
      361 tcgtcagggt gatgtacggc ggccggatct ctctcttcgt cggattcgcc tcttcggtga
      421 tcaccacact ggtcgggact ctgatcggtc tgctttccgg cttctacggc ggcctgatcg
      481 acaggttcct catgaggttc gtcgatgtta tgctttcgat tccaatgttt ccgatactcc
      541 tcactctgac gctcgtcttc ggttcgggta tcatgaacat catactcgtt ttaacggtct
      601 tcggctggat gggtgtagca cggctggtca ggggacttgt gctttccatt agagaaacgg
      661 aatacgtgat gtcggcccgg gcaatgggtg tgaggagcgc gagaataata ctcagacatg
      721 tactgccaaa cgtcataccg atagtgatcg tctcggccac gctgaacatg tcgtacgcaa
      781 tcctctcgga atcgtcgttg agctacctgg gacttgggat acagcctccg atgccctcct
      841 ggggcaacat gctccagaag gcgatgaact acatacttgg aacggcgaca ggtgccaatc
      901 cctggtggct gaccttcttt ccgggctttt tcatatttct gaccgtcctg agcgtgaact
      961 ttctgggcga tggacttaga gacgcactcg atccgaaatt cgtgagtgag agctgatgat
     1021 tatgcaagag atactaaaag ttgaaaacct aaagataacc ttcaaaacgc aactgggaaa
     1081 gataacaccg gtcgatggag tttcgttcgg actttccgaa ggtgagacgc tcggcatagt
     1141 tggcgaatcg ggctgtggca aaactataac ggccttctcg ataataaagc tcctccccaa
     1201 aaatgccata ctcggagatg aaacgaagat ctccttcagg ggcagggata tcgcgacact
     1261 ggagaagtcc gatctccaca agatccgcgg taaatccatc tcgatggtct ttcaggagcc
     1321 gatgacctct ttgaatcccc tctacactat aggctggcag atctcggagg tttacaagct
     1381 gcacgagggt ctggatgacg aagaggccat ggataggagt atcgagatgc tccgactcgt
     1441 cggcataccg gaaccggcca agcgggtcaa agagttcccg catcaactct ccggcggaat
     1501 gagacagaga gtgatgatag cgatggcact ggcctgcagc ccggcccttc taatcgccga
     1561 cgaacctacg accgcgttag acgtgactat acaggcccag gttctcgagc tcatgaacga
     1621 tctcaagaac aagctcgaca cggccacgat cataattacc cacgatcttg gcgtgatagc
     1681 cgagatgtgc gacagggtgc tggtgatgta cgccggccag atcgtcgaga gcgccgacgt
     1741 cttcagaata ttcgacaacc ccctacaccc ctacaccagg ggattgatcc actcgatacc
     1801 ccggatagag gtggagaagc gcgatcagaa gaggctcaac gttatcacgg gctatgtgcc
     1861 tcacccttct aaattcccga caggttgccg tttcgagccg cgttgcctca tggcgatgga
     1921 gaagtgcaag aaagagacgc cacagatcta cagccctgag gaagcccaca cggtgcgctg
     1981 ctggctggcc gaagggagtg agacaaatgg ctaacacccc catggtggag attcatcaat
     2041 tgaagaagtg gtttcccatc aagagtggct tcaaaaagaa atcagacctg aaggccgtcg
     2101 atgggg
//
LOCUS       DAOOLY010000352         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_66241_length_2104_cov_3.999512, whole genome shotgun sequence.
ACCESSION   DAOOLY010000352 DAOOLY010000000
VERSION     DAOOLY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66241_length_2104_cov_3.999512"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1942
                     /locus_tag="PLD15_12070"
     CDS             125..1942
                     /locus_tag="PLD15_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB64696.1"
                     /translation="MVVKRNRLVLLSSILIILLLTACPRNPENNDPVWNLPIGDRSVE
                     ETETLEITLSDHVSDPDGNSLSFSLSGKGSISAGGVYSYSPGIGEASTDFEESAVYAV
                     TITANDGKGGTASENFNVTVTPANDPPVWLTVPDKTVQEGSTLTILLTTYASDPDGDA
                     LAFDIPEGPGEIGGNNYEYTPSYEEAAMNFEEAAVYNVKLRAIDSKGATATTSFQVTV
                     TNKNRVPELSIPSPQYVTAGKTLALDVALFAYDPDGDAISFSKVAGVGSVTGSTFTYS
                     PALSDSGSRNATIRANDGNGGVTDQTVEININASHINNPPNVPGKPLPANNAMTQPAA
                     LTLTWFGGDPDGDPVTYDVYFGLSPAALSFKTNTSTRTYSTGSLNSSTYYYWQIVAKD
                     NKGASTTGPVWRFRTAPTQAFAETFESYSAGSIVGSFGPWYYQEYGSVTNPIITEIAG
                     HGKVIAFYGGNGYSVVYTPKVDPFKLGYLEYDVRLASTSIPRWGWIGPNNSLAYTGIG
                     YLDGKRAIVCWTQEGGSYHSERVMDIGPGIWYRVKVVFNMSTGGAAVYVDGTFKRSFS
                     FTPVPSGTNGVRMSTSNYNYSYVMFDNISLWVTDTGYVP"
BASE COUNT          567 a          521 c          530 g          486 t
ORIGIN      
        1 cttttcttaa tcggtgagtc tttggccgga aagatgattt attcctttcg agaaatagac
       61 tgtgaagaga gaatgaagtc ttccgtaaac cctaagaacg gccaaatccg gggaggtgtt
      121 atagatggta gtgaaaagga accgtcttgt actgctttca agtatcttga tcattctctt
      181 acttaccgca tgtcctcgca atccggaaaa caacgaccct gtatggaact tgccgatagg
      241 agacagaagc gtggaagaaa cggaaactct cgaaataact ctctccgacc acgtgtccga
      301 ccccgacggc aactctctaa gtttctcttt atcgggcaaa ggaagtattt ccgcgggagg
      361 ggtttactca tactccccgg gaataggcga agcctctacg gatttcgaag agtcggcagt
      421 ctatgccgtc acaatcactg caaatgacgg gaagggggga acggcaagcg agaacttcaa
      481 cgtgaccgta acaccggcaa acgatccccc ggtctggttg actgttcccg acaagacagt
      541 acaggagggt tccactctta cgatacttct aacgacttac gccagcgatc ccgacgggga
      601 tgctcttgcc ttcgatatac ccgaagggcc gggagaaatc ggcggcaaca actacgaata
      661 cactccttcc tatgaagaag ccgcaatgaa ttttgaagag gctgcagtgt ataacgtaaa
      721 actcagggcg attgattcga aaggcgcaac tgcaacgacg agtttccagg tgaccgtgac
      781 caacaagaac agagttccgg agctctctat accctctccg caatatgtca ccgccggaaa
      841 gactctcgcg ctcgatgtgg cactcttcgc gtacgatccc gacggagatg cgatctcttt
      901 ttcaaaggta gcaggtgtcg gctcagtaac cggtagtacg tttacttact ctcctgcact
      961 gagcgattcg ggctcaagaa acgcgaccat cagggcaaac gatggaaacg gaggggtaac
     1021 cgatcagact gtcgagataa atatcaacgc gagccacatc aataatcctc caaacgtccc
     1081 cgggaaaccc ttgccggcaa acaatgcaat gacacagcct gccgctttga ccctgacgtg
     1141 gtttggagga gatccggacg gtgatccggt cacgtacgac gtatacttcg gtctctctcc
     1201 tgcggcgctg agctttaaaa caaatacaag caccagaacg tattcgaccg gctctctcaa
     1261 cagctctact tattattact ggcagatagt ggcgaaggac aacaagggag cctcaacgac
     1321 cggaccggta tggcgtttcc gcactgcacc gacgcaagcc tttgccgaga ctttcgaatc
     1381 atattctgca ggttcaatcg tcggctcatt tggaccgtgg tattaccagg agtacggcag
     1441 cgttacaaac cccataatca cagagatcgc cggccatgga aaggtaatcg ctttctacgg
     1501 cgggaacggt tacagcgtcg tatacactcc aaaggtagat ccattcaaac taggctatct
     1561 ggaatacgat gtcagactag cctcaacatc gattccaagg tggggctgga tcggtcctaa
     1621 caactcgcta gcgtatacag gaataggcta tcttgatgga aagagagcca tcgtctgctg
     1681 gacacaagag ggcggctcct atcactcgga aagagtaatg gatattggtc cgggaatctg
     1741 gtatagagtc aaagtggtct tcaatatgtc aacgggtggc gcggcagtat atgtagatgg
     1801 aacgttcaag aggagttttt ctttcacacc ggttccctct ggaacaaatg gggtaagaat
     1861 gtccaccagt aattacaact attcgtacgt catgttcgac aacatcagct tgtgggtcac
     1921 agataccggt tacgtgccgt aaaccgtggg gggtttcata caatcagtcg tcatgttcga
     1981 aagtagactc tgtcatgtct gaagaaggtg ttcttctctc aagagagaga gatagttttt
     2041 aaagagatct ctcgagtagt tcacaatgtc gaatggactt ctgctcctcc ataatctggt
     2101 tgcc
//
LOCUS       DAOOLY010000353         2099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_66479_length_2099_cov_4.436888, whole genome shotgun sequence.
ACCESSION   DAOOLY010000353 DAOOLY010000000
VERSION     DAOOLY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66479_length_2099_cov_4.436888"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLD15_12075"
     CDS             <1..464
                     /locus_tag="PLD15_12075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPB64697.1"
                     /translation="GTGIIMNNHMDDFVPTPGSANSVEPGKRPLSSMSPTLVLDPQGR
                     PYMTLGSPGATRIITTVAQVISNIIDHGMTIQQAILAPRVFRMASGSMSIEGRISINA
                     YNKLLEMGHQLTLRGDYDAYFGGVHGVLYNYDIGILFGGADPRRDGQAFAF"
     gene            743..1870
                     /locus_tag="PLD15_12080"
     CDS             743..1870
                     /locus_tag="PLD15_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPB64698.1"
                     /translation="MKKRYVQFIVFISVVLVVVLLSGFSFTSMWKDPPIVRGPGVSEV
                     KMLSDWLPSLSGTKGDTEVYIIRGSEPGGNFLLLGGNHPNEPGGTLAAVLMIENVSSL
                     AGTVYIIPRANHSAFTHNDPMEGAPQFFSIELPDGSRRTFRFGSRRTNPVSQWPDPTI
                     YNHPTGSTLGGSEISNLNRAFPGREDGIITERVAAAIMNIVKQEKIDLVCDLHESSPE
                     YPVNNAIVFHQEAAELAIMTQMMLEMEGVDIRLEESPPSLRGLTHREIGDNSDAYVVL
                     LEVANPSQGRLRGKTTEELVTTGVDKYYLQASKDGKLFAPYDETGYPLELRVARHVTT
                     VRVLLDSWNMMFPDRMIMLSDVPAYNAILSDGLGAFLKPVS"
     gene            1896..>2099
                     /locus_tag="PLD15_12085"
     CDS             1896..>2099
                     /locus_tag="PLD15_12085"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPB64699.1"
                     /translation="MKKHVLVVMLVAVLFTGLIATITAPTVASSNGMVAAVNNYAAEA
                     GAKILEMGGNAIDAAIAVSLALGV"
BASE COUNT          534 a          534 c          541 g          490 t
ORIGIN      
        1 cgggaacggg aataataatg aacaaccaca tggacgactt cgttccaaca ccgggttcgg
       61 cgaactcggt agaaccaggt aagagacctc taagcagtat gagccccacg ctcgttctcg
      121 atcctcaggg aagaccttac atgactctcg gttcacccgg agccacgaga atcataacga
      181 ccgttgccca ggtgataagc aatatcatcg atcacggaat gacgatccag caagccatac
      241 tcgctccgag agtcttcaga atggcctccg gttctatgtc tattgagggc aggatatcca
      301 taaacgccta caacaaactg ctcgagatgg gtcatcagct gaccttacgt ggtgattacg
      361 atgcctactt cggaggcgtt cacggcgtac tgtacaacta tgacatcggt attctcttcg
      421 gaggagccga tcccagacgt gacggtcagg ccttcgcctt ctgataaaaa tcgatcggtc
      481 acgctgaatg tctttgccag aaagcggaac agacctgaat aaaggtctgt tccttttcca
      541 ctgttaacag aaagtcaaca aattaacttt tcaaacttat ccagataatt gacggtcgaa
      601 tagtataatt gtatagttgt ctttctttac tggattaatg tagcccttga atcggttttg
      661 tgcagatctc tgttcaggat tttcgggcac cggcaaatcc tggaaagtga tacccttcgg
      721 cggcgaaagg attggtgatc ctttgaaaaa gagatacgtt cagttcattg tgtttatctc
      781 tgttgttctt gttgtagttc ttctctcggg cttttctttc acttcgatgt ggaaagatcc
      841 tccgatagta cggggtccgg gtgtctcgga agtcaaaatg ctcagcgact ggttgccctc
      901 actttcagga acgaaaggcg acacagaagt ctatatcata aggggcagcg agccgggagg
      961 aaacttcctt ctcctgggag gaaaccaccc caacgaaccg ggcggaacgt tggcggccgt
     1021 tctcatgatc gagaacgtct cgtctcttgc tggaacggtg tatataatcc cgcgggccaa
     1081 ccacagtgcc ttcacccaca acgaccccat ggaaggggct ccacagtttt tcagcataga
     1141 acttcccgac ggctccagga ggaccttcag gttcgggtcc agaagaacca acccggtatc
     1201 gcaatggccc gatcctacca tctataacca tcccacaggt tctacgctcg gcggtagcga
     1261 gatctcgaac ctcaacaggg cctttccggg ccgtgaggac gggatcataa ccgaacgggt
     1321 ggccgccgca ataatgaata tcgtgaaaca ggaaaagata gatcttgtat gcgacttaca
     1381 tgagtcttcg ccggagtatc cggtgaacaa cgccatagtc tttcaccaag aagccgcaga
     1441 actggccatc atgacacaga tgatgctcga aatggaaggc gtggatataa ggcttgaaga
     1501 gtcgccgccg tctctgcgcg ggttgacgca cagagagata ggcgacaatt ccgatgcgta
     1561 cgtggtactt ctggaagttg ccaatccttc acagggcagg ctccgtggaa agacgacaga
     1621 ggagctcgta actaccggcg tggataaata ttatctccag gcttcgaagg acggcaaact
     1681 tttcgcaccg tacgatgaaa ccggataccc actggagcta agggttgcgc gtcacgttac
     1741 caccgtgaga gtccttctcg attcctggaa catgatgttc ccggatcgaa tgataatgct
     1801 ctccgacgtg cccgcgtaca acgcgatact cagtgatggt ctgggcgctt ttctaaaacc
     1861 tgtttcataa gactttctga attggagggg aaaaaatgaa gaaacatgtg cttgttgtta
     1921 tgctcgtagc cgtattgttc accggcctta tcgcaaccat aaccgcaccg acggtagctt
     1981 ccagcaacgg aatggtggcc gccgtcaaca attatgcggc ggaggccgga gccaaaatac
     2041 tagaaatggg gggcaacgcc atcgatgcgg ccatcgcggt cagtctggcc ctgggtgtt
//
LOCUS       DAOOLY010000354         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_67513_length_2077_cov_2.787834, whole genome shotgun sequence.
ACCESSION   DAOOLY010000354 DAOOLY010000000
VERSION     DAOOLY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67513_length_2077_cov_2.787834"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1030)
                     /locus_tag="PLD15_12090"
     CDS             complement(188..1030)
                     /locus_tag="PLD15_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB64700.1"
                     /translation="MIEGAFRKLDELSKVDVVNLLNIIFEDYALRMQWNLESFDRDVH
                     ENSISLSDSFVMDMKDEPVGIVLLSFRDKRARIDLMGVIPSFRRSGVGFQMVDEAVKI
                     CKWRGSDSVVLEVLKKDSRAMGFYRKFGFREKRDLVTFFLRKCGNNGYVLKRTTPDVV
                     MEKALCTMGKYGRQPEWQREPSNFSRLDLYNFDRIVDESEREIGYCVWGQKEDVMYVV
                     DSGPSERSDFHEVLQAVCGIAKGNGYLLLFPTVPEEDALFKAAEKLEPEILLVQTEMI
                     YKLH"
     gene            complement(1027..1416)
                     /locus_tag="PLD15_12095"
     CDS             complement(1027..1416)
                     /locus_tag="PLD15_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HPB64701.1"
                     /translation="MNRKASILVWCDQEALDVLRERIPGIVIVDCRGFEQYTVESILK
                     GENIERELFSNGGRRGDENEAYCFVVFDGLKNELVGRAVSFIKKAVGRRWIFATTTEN
                     NISWKLKNLQEELIEEHNYFETRGDKK"
     gene            complement(1427..>2077)
                     /locus_tag="PLD15_12100"
     CDS             complement(1427..>2077)
                     /locus_tag="PLD15_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB64702.1"
                     /translation="YVKADVMGRPLGQVVEEVRSIIDGRDGLAECQLKGQGDLIEQII
                     EQFGFALLAGIVFMYLIIGAQFESLVYPLVIFCTLPMALVGVVVVSYLFNLTINISSL
                     VGVLTLAGVIVNNGIVMITQINQLRKGGMQKREAILEGAGRRARPILMTSLTTIVALL
                     PTAFIKSEGSELESPLALIIAGGLLVGTLFTLLLTPVLYDLIDNLSVRFKKKSPIQ"
BASE COUNT          513 a          565 c          436 g          563 t
ORIGIN      
        1 ccacgcattc accggaggaa gctgggatac tacggtggtt gcaggtgacg attcaaggtt
       61 cgcggttctt ggtgacgaag agtttttcga tattctaata gaaaaatttc ccccggaggg
      121 gttcagactg ctcgaatatc tgctcggcta ttcaagagag atgcggggct attgagcccc
      181 gtttttttca atgaagctta tagatcatct cagtctgcac gagtagtatt tccggttcga
      241 gtttttcggc ggctttgaac aacgcatctt cttccggaac ggttggaaac aagagaagat
      301 agccgttgcc ttttgctatg ccacagacag cttgaagcac ctcatgaaaa tcggaacgtt
      361 ccgatggacc ggagtcgacc acatacataa catcctcttt ctgaccccag acacagtatc
      421 cgatttccct ttcgctctca tctactattc tgtcgaagtt gtataaatcc aggcgggaaa
      481 aattcgatgg ctcgcgctgc cattcaggct gtctgccgta cttacccata gtacagagag
      541 ccttttccat tactacgtcg ggtgtggttc tcttaagaac gtaaccgtta ttgccgcatt
      601 ttctgaggaa gaaagtgacc aggtccctct tctcgcgaaa accaaacttt ctgtaaaagc
      661 ccatggccct cgaatccttc ttgagaacct ccagcactac gctatcgctg cccctccatt
      721 tacagatctt caccgcctcg tcaaccatct gaaaaccgac gccgctcctc ctgaacgatg
      781 gaataacgcc cataagatcg attctggccc gcttgtctct gaaactgagc agaactatcc
      841 cgaccggttc atccttcata tccatgacga acgaatcgga cagcgagata ctgttctcgt
      901 ggacatcgcg atcgaagctc tcgagattcc actgcattcg caacgcataa tcttcgaaaa
      961 tgatgttgag gagattcacc acatcgacct ttgagagttc atccagcttt ctaaaagctc
     1021 cttcaatcac tttttatcgc ctctcgtttc gaaataattg tgttcctcta tcaactcctc
     1081 ctgaagattc ttcagcttcc aggatatatt gttctccgtc gtggtggcga agatccatct
     1141 tctcccgaca gcctttttga tgaaactcac cgcacgacct acgagctcgt ttttgagacc
     1201 atcgaagacg acgaagcaat acgcttcgtt ctcgtccccc cgccttcctc cgttggaaaa
     1261 gagttcccgc tcaatgttct ctccttttag aatggattcg acggtgtatt gttcaaatcc
     1321 gcggcaatca actattacga ttcccggaat tctctctctc aaaacatcca gtgcttcctg
     1381 gtcgcaccag accaaaatac tcgcttttcg attcactgca aacacctcat tgtatggggc
     1441 ttttcttttt gaatctcacg gagagattgt ctatcaagtc gtaaagaaca ggggtcagca
     1501 aaagcgtaaa gagagttccc accagcaatc ctccggcgat tattagagcg agggggctct
     1561 ctaattccga accttctgac tttatgaaag ccgtagggag taatgcgaca atagtcgtca
     1621 gagatgtcat cagaatcggc cttgcccgcc tgccagcccc ttcaaggata gcttctctct
     1681 tttgcattcc acccttcctc aactgattta tctgagttat catcactatt ccgttgttaa
     1741 ctatgacgcc tgccagtgta agtactccca ccaggctgct gatgtttata gtgagattga
     1801 acaggtaaga aacgacgacc acgccgacaa gagccatcgg aagggtgcag aagataacca
     1861 gtggatacac cagtgattcg aactgggcgc cgattattag atacatgaag actatccctg
     1921 ctaagagagc aaagccgaac tgctctatta tctgctctat aaggtctccc tgacctttca
     1981 gctgacattc ggccaggcca tcccggccgt ctatgattga tctcacttct tctacaacct
     2041 gtccgagagg tctgcccatc acgtcggctt tcacata
//
LOCUS       DAOOLY010000355         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_67708_length_2073_cov_3.871160, whole genome shotgun sequence.
ACCESSION   DAOOLY010000355 DAOOLY010000000
VERSION     DAOOLY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67708_length_2073_cov_3.871160"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>2073
                     /gene="lon"
                     /locus_tag="PLD15_12105"
     CDS             105..>2073
                     /gene="lon"
                     /locus_tag="PLD15_12105"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB64703.1"
                     /translation="MAQKFELLEKAAIRSERKIEIPDILPAIPTRTNMLIYPSSVMPL
                     YVGREKSLSALEESIGKYDQLVFLVSQRDITKEEPELGDLFEVGTIGRIVQLMKMPDG
                     NYKILVEGLARAKISGVVEEENALIVKLESLKSRTRKSKMLEALVRKVKELTQKYVSM
                     SRRFPDEALMALEDTSNADKFADFVSSIVPFALEEKQKLLEEVEPVERLKILMELLSR
                     EIEILSLEEELDKRVKEKIEQGQKEYYLREKMRAIQEELEGEEDEEIKELKERAASPD
                     LSAEVREKAEQEISRLEKMSPYSAEATVVRTYLDWLLNLPWSQETEDEIKIKDVRRML
                     DSNHYGLQDVKERILEFLAARKYSEKLRAPILCLVGPPGVGKTSLGRSVAEAMGRKFG
                     RISLGGMRDEAEIRGHRRTYVGALPGRIMQMIRKLGSRNPVIVLDEVDKMGISFQGDP
                     ASALLEVLDPEQNSNFTDHFLEVSFDLSRVLFITTANVLYSIPDALRDRMEVIEIPGY
                     TESEKYHIAKDHILPKLLDEYNMKDRSFKITGSAIKGTIRDYTREAGVRQLDRSLGKI
                     IRKATLKMAEGEESINVGVNDLGEFLGVPLFKDSDFRKKPEVGVVTGLAWTSVGGEIM
                     YIEVLPVPGKGKTIITGQLGDVMKESAQIAAS"
BASE COUNT          563 a          484 c          621 g          405 t
ORIGIN      
        1 gaaagagtcg tgtccctgtc atcgcttgat gagaaaaccg atggattgct ctcttttttg
       61 aaatgaaaag acggtatctt accaaatgaa aggaaggtgt tctggtggca caaaaattcg
      121 aattacttga gaaagccgca attaggagcg agagaaagat agagataccc gatatattgc
      181 cggccattcc aacgagaacc aatatgttga tatacccctc ttcagtgatg ccgctctacg
      241 tcgggaggga gaagtccctt tcggcgctcg aagaatcgat cggcaagtac gatcaactgg
      301 tcttcctcgt gtctcagaga gatataacca aagaggaacc ggaactgggc gatctcttcg
      361 aagtcggtac tataggccgc atcgtccagt tgatgaagat gcccgatggc aattacaaga
      421 tcctggtcga gggactggca cgggcgaaga tctcgggcgt agttgaagag gagaacgctc
      481 tgatcgtaaa gcttgagagc ctgaagtcca gaaccagaaa gagcaagatg ctggaggcac
      541 tggttcgcaa agtcaaggag ctgacgcaga aatacgtttc gatgagccgc aggttcccgg
      601 atgaagctct tatggctctt gaagacactt ccaacgccga caagttcgcc gatttcgtct
      661 cttcgatcgt tcccttcgct ctggaggaga agcagaagct gctcgaagag gttgagccgg
      721 tagagaggtt gaagatactc atggaactcc tgagccgcga gatagagatc ctctcgctcg
      781 aggaggaact ggataagcgc gtgaaggaga agatcgaaca gggtcagaag gagtactatc
      841 ttcgcgaaaa gatgcgtgcg attcaggaag agctcgaggg cgaggaagac gaagagataa
      901 aggaattgaa ggaaagggcc gcctctcccg acctgtctgc cgaagttcgc gagaaggccg
      961 aacaggagat atccagactg gagaagatgt ctccctactc ggccgaggcg acggtggtga
     1021 gaacgtatct ggactggttg cttaaccttc cctggtctca ggagacggag gacgagataa
     1081 agataaaaga tgtacgtaga atgctggaca gcaaccatta cggattgcag gacgtcaagg
     1141 agcggatact ggagttcctg gctgccagaa agtactcgga aaagctcagg gctcccatac
     1201 tctgtctggt gggtcctcca ggtgttggca aaacctctct ggggcgatcc gtagccgagg
     1261 caatgggcag aaagttcgga agaatctctc tgggcggtat gagagacgag gccgaaatac
     1321 gcggccacag gaggacctac gtcggcgccc ttccgggcag gataatgcag atgattcgca
     1381 agctcggctc taggaacccc gtgatcgtgc tggacgaggt cgacaagatg ggaataagct
     1441 tccagggtga cccggcttcg gcgctgctgg aggttctcga tcccgagcaa aacagcaatt
     1501 tcaccgatca ctttctggag gtatccttcg acctctccag ggtgttgttc ataactacgg
     1561 cgaacgttct gtattccata cccgacgccc tcagggacag gatggaggtc atcgaaatac
     1621 ccggatacac cgagtcggag aagtatcaca tcgccaaaga ccatatactg cccaagctgc
     1681 tcgacgaata caacatgaag gacaggagct tcaaaatcac cggatcggct ataaagggaa
     1741 cgataaggga ctacaccaga gaggccggtg tgagacagct cgaccgcagc ctgggaaaga
     1801 taatccggaa ggcgaccctg aagatggccg aaggcgagga gagtataaac gtaggggtga
     1861 acgatcttgg cgaattcctg ggagtaccgc tcttcaagga cagcgatttc cgaaagaaac
     1921 ccgaggttgg tgtggtgaca ggcctcgcct ggacctccgt cggaggggag atcatgtaca
     1981 ttgaggtgct tcccgtgccg gggaagggaa agaccatcat caccggccag cttggcgatg
     2041 ttatgaagga atcggcgcag atagccgcct cgc
//
LOCUS       DAOOLY010000356         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_69317_length_2041_cov_4.235146, whole genome shotgun sequence.
ACCESSION   DAOOLY010000356 DAOOLY010000000
VERSION     DAOOLY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69317_length_2041_cov_4.235146"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PLD15_12110"
     CDS             <1..1340
                     /locus_tag="PLD15_12110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB64704.1"
                     /translation="DPTTMPLNVGIVGKTYREKKSFVLGDIQESEHAYKTNNKYHGGL
                     SVPVGNIGVFQAMSMEKNAFADEDVKLAELLISHANEAIKRIETEQEMAFMSLHDRLT
                     GLYNRVYFEEEIQRLNYSRLYPISIVSADIDGLKLINDTMGHSAGDDLLRSFAEILKA
                     NMRSSDVVSRFGGDEFAAILVSTDRPTAERVLERIRKAVARHNESRSGPFLSFSMGVA
                     TSDNGESSLLDCLKLADDLMYRDKLSRSNSVRSQMVNTLLLTLAEKDHISGGHASRLQ
                     ELALKLGRRMGINSSQLVDLSLFAQVHDLGKVGIPDRILFKPGKLTQEEWEVMKLHPE
                     KGYRIAISSQDLSTVADLILRHHERWDGKGYPLGLEKTEIPIECRILSILDAYDAMTN
                     DRPYSKARSHSEALKEIERCAGTQFDPQIVEEFIKMLNGGTQHLQGPVPSENS"
     gene            complement(1383..>2041)
                     /locus_tag="PLD15_12115"
     CDS             complement(1383..>2041)
                     /locus_tag="PLD15_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64705.1"
                     /translation="RKVYGTVIKTEVLHGINLSFEEGSFNSIIGQSGSGKSTLLNIMG
                     TLDLPTSGEITINGKRTDTMSKNDLAAVRNETVGFIFQFHYLLPEFTAFENILMPYKI
                     KSLRSSREVIERAKELMEVVEISKVKDNLAPNMSGGQQQRTAIARALINNPKVILADE
                     PTGNLDSDTSAKVFRLMRDLNAKYGTTFIVITHDRRIAEETDRVVEIRDGNIFNDVRR
                     "
BASE COUNT          512 a          504 c          536 g          489 t
ORIGIN      
        1 aagacccaac gaccatgccg ctgaacgtgg ggatagttgg aaagacttac agagagaaaa
       61 agagctttgt tcttggcgat atacaggaat cggaacatgc ctacaagacc aacaataagt
      121 accacggggg attgagcgtt ccggtgggaa acattggcgt ttttcaggcc atgtccatgg
      181 agaaaaatgc gttcgccgac gaagatgtca agctggccga gttgcttata tcgcacgcaa
      241 acgaagcgat aaagagaata gagaccgaac aagagatggc tttcatgtcc cttcacgaca
      301 ggctaaccgg tctttacaac agggtctatt tcgaggagga gattcagagg ctcaactatt
      361 cgagactcta cccgatctcg atagtctcgg ccgatataga tgggctcaag ttgataaacg
      421 acacgatggg ccactccgcc ggcgacgatt tgttgaggtc tttcgccgaa attctgaaag
      481 ccaacatgag gagttccgac gtcgtttcgc ggtttggagg ggacgagttc gccgccattc
      541 tggtatctac cgaccggcca acggccgaaa gagttttaga aaggataaga aaggccgttg
      601 ccaggcacaa cgaaagccgg agcggaccgt ttctcagctt ctcgatgggc gtcgccacga
      661 gcgataacgg tgagagctct ctgctggatt gcctgaaatt ggccgacgat cttatgtatc
      721 gcgacaaact gagcaggagc aacagtgtga gaagccagat ggtgaatact ttgctgctaa
      781 ctctggcgga gaaggatcat atatccggtg gtcatgctag caggttgcag gaactggctc
      841 tcaaactggg tagaaggatg gggatcaact ccagccagct ggtggatctc tcgctgtttg
      901 cccaggtgca cgatcttgga aaggttggca tccccgacag aatacttttc aaaccgggaa
      961 aactgacgca agaggaatgg gaagtgatga agctccatcc cgaaaagggt tacaggatag
     1021 ccatatcctc ccaggacctc tcgaccgtcg cagatctcat actcagacac cacgaaagat
     1081 gggatgggaa agggtatccg ctcggtctgg aaaaaactga gatccccatc gaatgcagga
     1141 ttctttcgat tctggacgcc tacgacgcca tgaccaacga tagaccttac agcaaggcaa
     1201 ggagccattc ggaggctctc aaagaaatcg agagatgtgc aggaacccag ttcgatccac
     1261 agatcgtcga ggagttcatc aaaatgctga atggcggtac ccaacacctt caaggtcccg
     1321 tcccgtcaga gaactcctga atcggtgaaa caaccgcccg gcctccggca aacggttctc
     1381 gactatcttc tcacatcgtt gaatatattg ccgtctctta tttcaactac cctgtcggtc
     1441 tcttcggcga tccttctatc gtgcgtgatc actatgaaag tcgttccgta tttagcgttc
     1501 agatcgcgca tcagtctgaa caccttggcc gacgtatcgg aatccaggtt tcccgtgggc
     1561 tcgtcggcaa gtatcacttt tggattgttg atcaacgccc tcgcaatggc cgttctctgc
     1621 tgctgaccgc ccgacatgtt gggggcgagg ttatctttca ccttgcttat ttcgactact
     1681 tccatgagtt ctttagccct ttcgattact tctctcgaag atcggagcga ctttattttg
     1741 tatggcatca atatgttttc gaaggcagtg aactccggta gcaggtagtg aaactggaat
     1801 atgaacccta ccgtctcgtt cctgacggcc gccagatcgt tcttgctcat ggtgtcggtc
     1861 cttttaccgt ttatggttat ctcgccgctg gttggaagat cgagagtacc catgatgttc
     1921 aagagggtgg atttcccgct gccggactgg ccgatgatag aattgaagga gccttcttcg
     1981 aaactcaggt tgatcccgtg aagaacttcg gtcttgatta cagttccgta cacctttcta
     2041 a
//
LOCUS       DAOOLY010000357         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_69770_length_2031_cov_3.802632, whole genome shotgun sequence.
ACCESSION   DAOOLY010000357 DAOOLY010000000
VERSION     DAOOLY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69770_length_2031_cov_3.802632"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLD15_12120"
     CDS             complement(<1..706)
                     /locus_tag="PLD15_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPB64706.1"
                     /translation="MIVLKLERHPLNPLLSPISRHHWESRFVFNCAVIKRQGLFHMLY
                     RAQGEDMVSRMGYAVSTDGVRFNRMENPVFTPASQWELYGVEDPRLTELEGRIYLQYT
                     AYSPRGVRISMASTENFFQWERHGVIIPDVDNKDAALFAEKIAERYVMFHRIEPDMYL
                     AFSEDLRKWTDFVSIAGPRKDGWDNLKIGVGALPIKTEFDWLVLYHGVENTPRPTYRL
                     GFMLLDLENPRRVLKRS"
     gene            complement(713..1531)
                     /locus_tag="PLD15_12125"
     CDS             complement(713..1531)
                     /locus_tag="PLD15_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64707.1"
                     /translation="MKKFWQISLYVFLFVASILWIYPYIWMVLASFKPANEIYGQFLP
                     SRLTLEHYRFILQAADQMDRPFMLALGNSLLISVAVTLGVLVTSALVGFALSRLYFKG
                     RNMIFNFIIFQMIFPGFMFIVPLYVLIKNLGLIDTRTAVILPSLVSAWGVFMFTQSFR
                     AVPGEYLEAAKMDGANDLWIIARVLVPLTSSTASIVGLFTFIGIWDNFMWPLIIVSDY
                     SKMPLSVLLASFNMQYGSYLGPVLAGSVLQTMPMVVIFLIFRKYFLQGISMSLK"
     gene            complement(1528..>2031)
                     /locus_tag="PLD15_12130"
     CDS             complement(1528..>2031)
                     /locus_tag="PLD15_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64708.1"
                     /translation="KLFAVNGPLNDLTRALFGVTIPWFSSPQLATLSIAIMVTWKFIG
                     YYGLIFFSGLQAIPQSLYEAAELDGAGKWTKFFKITVPLLNPSIVMVLVLSLTLTFGI
                     FTEPFLITGGGPMRTTYTFQMLIYSTAFQKINPGYASSLAIVVALLSYGCVLLTRKLV
                     EREVELA"
BASE COUNT          493 a          551 c          558 g          429 t
ORIGIN      
        1 gcgaacgctt cagaaccctg cgcggatttt ccagatcgag caacatgaag ccgagtctgt
       61 acgtcggcct gggggtgttt tccacgccgt ggtagagtac gagccagtca aactccgtct
      121 ttattggcag agcgccgaca cctatcttga ggttgtccca gccgtctttt cttggcccgg
      181 caatagacac gaagtcggtc cactttctca aatcctccga gaatgcgaga tacatatccg
      241 gttctattct gtggaacata acgtatcttt cggcgatctt ttcagcaaaa agcgcggcgt
      301 ccttgttgtc cacgtccggt attatcacac cgtgcctctc ccattgaaag aagttctccg
      361 tcgaagccat ggaaatcctg accccgcgtg gagagtaggc cgtgtactgc agatagatcc
      421 ggccctccag ttccgtcagt ctgggatctt ccacgccgta aagttcccac tgactggccg
      481 gcgtgaagac ggggttttcc atcctgttga agcgaactcc gtcggtcgat acggcgtatc
      541 ccatcctcga gaccatgtcc tcgccctgag ccctgtacag catgtgaaac agcccctgac
      601 gctttatcac ggcgcagttg aagacgaacc tcgattccca gtggtgccgg gaaatcggcg
      661 aaagcagagg attaagcggg tgtctttcga gtttcagcac aatcatcact ccctatttca
      721 atgacataga tattccctgc aggaagtatt ttctgaatat tagaaatata acgaccatcg
      781 gcatagtttg caggaccgag ccggccagca ccgggccgag gtagctgccg tactgcatgt
      841 tgaagctggc cagcaaaacc gatagcggca tcttcgagta atcgctgacg attatcaggg
      901 gccacatgaa attgtcccat atgccgatga aggtgaaaag acccactatc gaggcggtgg
      961 agctggtgag gggaaccagc accctggcga ttatccagag atcgttggct ccgtccatct
     1021 tcgccgcctc cagatactcg cctggaacgg cccggaaaga ctgggtgaac atgaagacgc
     1081 cccaggccga aacgagcgag ggcaggatca cggccgtcct tgtgtctatc aagcccaggt
     1141 tctttatgag cacgtaaagc ggcacgatga acatgaaccc tgggaagatc atctggaaga
     1201 ttatgaagtt gaagatcatg ttccttccct tgaagtacag cctggacagg gcgaatccca
     1261 ccagcgccga tgtaacgagg actcccagcg taacggcgac cgagatgagt aaagagtttc
     1321 ccagggccag catgaagggg cggtccatct ggtcggcggc ctgaaggatg aacctgtaat
     1381 gctccagtgt gagccggctg ggcagaaact ggccgtagat ctcgttcgcc ggcttgaaag
     1441 aggccagtac catccagatg tagggataga tccagaggat cgaggcaaca aagagaaata
     1501 cataaagcga gatctgccag aatttcttca tgccagctcc acctccctct cgaccagctt
     1561 tctggtcagg agcacgcagc cgtagctcag tagcgccaca acgatcgcta gcgaggaggc
     1621 gtaccccgga ttgatcttct ggaaggccgt cgagtagatt agcatctgga aagtgtaagt
     1681 cgttctcata gggccaccgc cggttataag gaagggctcg gtgaatatcc cgaaggtgag
     1741 ggttaacgac agcaccagaa ccatcactat cgaaggattg aggagcggga cggtgatctt
     1801 gaaaaacttt gtccactttc ccgccccatc cagctcggcc gcctcgtaca gagactgggg
     1861 aatcgcctgc aagcccgaga agaagatcaa cccgtagtag cctatgaact tccatgtgac
     1921 catgatggcg atcgagagcg ttgccagttg cgggctgctg aaccagggta tcgtaacgcc
     1981 gaagagggcc ctcgtcagat cgttcaacgg cccgttcacc gcgaagagtt t
//
LOCUS       DAOOLY010000358         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_70673_length_2013_cov_3.825332, whole genome shotgun sequence.
ACCESSION   DAOOLY010000358 DAOOLY010000000
VERSION     DAOOLY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70673_length_2013_cov_3.825332"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="PLD15_12135"
     CDS             complement(<1..1374)
                     /locus_tag="PLD15_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HPB64709.1"
                     /translation="MTAKGERKWILEIGQGIYNELGGVEALEGIILDISDRKEIENNL
                     RYSNEHDNLTGLYNRNYLESLLESDTKKPAAEKRALVSVNLSAVQSLTMTYGFHYTQE
                     LIKKVADTLNQYCTERRVLFSTHENRFTFYIRGYEDRNELVEFSETLSKALESLLAVE
                     RTGGGIGVIEIERDNECDVDLLMRRLLITSEKAIDTNNKDVGICFYDTEIEMQTAREE
                     EVTRELTTIAADESNGGLFLQYQPILDLKSNRICCYEALARLNIEGLGPVPPLEFIPI
                     AEKTKLIIPIGRKIMLQAFRFLDKLKKRGYETISVSINVSAIQLLRNDFASSLFEMID
                     EMQVNPESISLEITESVFTSNYEEVNRVLGELMKAGIHIAIDDFGTGYSSLSRERDLN
                     VNCLKIDRSFISRLMYLKPEDAITGDIVSIAHKLGHLVVAEGVEHEKQREYLLEYGCD
                     NIQGYLIS"
     gene            complement(1429..>2013)
                     /locus_tag="PLD15_12140"
     CDS             complement(1429..>2013)
                     /locus_tag="PLD15_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB64710.1"
                     /translation="TVFQQAPIGIAISHSDQPLDSVSKAVTRFNPMFEQITGRTKEEL
                     IELGWGKITHSDDLEEDLKKYRKLKSGEVKSYSMEKRYIKPDGSVVWVYMTVAPLVLL
                     NDDQYNHICLVQDITERKAIEADLLESERSKSVLLSHLPGMAYRCNYDREWTMQYVSD
                     GCFELTGYRPENLVNNRDLAFKDLVAPEFRESLW"
BASE COUNT          447 a          546 c          435 g          585 t
ORIGIN      
        1 gctgatcaga tacccctgta tattgtcgca cccgtattcc aataaatact ctctctgctt
       61 ttcgtgctcc accccttccg ccacgaccag gtgtcccagc ttatgcgcta tcgagacgat
      121 atcgccggtg atggcgtcct cgggtttgag gtacatcaat ctggaaataa aagatctgtc
      181 gattttcagg cagttcacat tcaagtccct ctccctggaa agagaggagt agcccgtacc
      241 gaagtcgtct atggctatat ggattcctgc cttcataagc tcaccgagaa cgcggttgac
      301 ttcttcgtaa tttgaagtga ataccgactc cgtgatttcc aggctgatac tctccggatt
      361 cacttgcatt tcatctatca tctcaaaaag actactggcg aaatcgtttc tcaacagctg
      421 aattgccgat acgtttatcg aaacggaaat tgtttcatac cccctcttct tcaatttgtc
      481 caggaaacga aaggcctgga gcataatctt ccgaccgatc gggattatga gctttgtttt
      541 ctcggcgatc ggaatgaact ccagaggcgg taccggaccg agcccctcga tattgagcct
      601 ggccagagct tcgtagcagc aaatcctgtt cgattttaga tccaggatcg gctgatactg
      661 caggaacagt ccgccattac tctcatcggc cgcaattgtg gtaagttcgc gggtaacttc
      721 ctcctcgcgg gccgtctgca tctcgatctc cgtgtcgtag aagcatatgc cgacgtcctt
      781 gttgttggta tcgatcgctt tttccgatgt gatcagaagt ctcctcataa gcagatccac
      841 atcgcattcg ttgtctcgtt cgatttcaat gactcctata ccgccgccgg tcctctccac
      901 ggcaagcaga gactccagcg cttttgaaag ggtttcggag aattccacca gttcgttcct
      961 gtcttcgtag ccccttatgt agaaagtgaa tcgattttca tgtgtgctga aaagtacgcg
     1021 cctctctgtg caatactggt ttagagtatc tgcgactttc tttatgagtt cctgcgtgta
     1081 atgaaaccca taagtcatag ttaacgactg cacggcgctc aaatttacgc tcacgagcgc
     1141 tcttttctcg gccgcaggct tcttcgtgtc gctctcgaga aggctttcca gataattccg
     1201 gttgtacagg cccgtcaagt tatcgtgttc attgctgtat ctgagattat tctcgatttc
     1261 cttccggtcg gagatgtcga gtattattcc ctcgagagcc tccactccgc ccagctcgtt
     1321 gtagataccc tgcccaatct ccagaatcca tttccgctcc cctttcgccg tcactatctg
     1381 gtattcataa tttaaagtaa ttcgcctttc gactgctctt cgccactctt accagagcga
     1441 ttcgcgaaat tccggcgcga ccaggtcttt gaaggccagg tccctgttat tgacgaggtt
     1501 ttcagggcga taaccggtca attcaaaaca gccgtcggaa acgtattgca tagtccattc
     1561 tctgtcatag ttgcacctgt acgccatacc gggaaggtgg gaaagaagaa cggatttgct
     1621 gcgttcgctc tcgagcagat cggcttctat cgctttacgc tctgtaatat cctgaaccag
     1681 acatatatgg ttgtattgat cgtcgttcaa caaaacgagc ggagccaccg tcatatatac
     1741 ccatacaacc gaaccatccg gcttgatata tctcttctcc atcgaataac tcttgacttc
     1801 gcccgacttc agtttcctgt atttcttcaa atcctcttca agatcatcgg aatgtgtaat
     1861 cttcccccag cccagctcta tgagttcttc cttcgttcta ccggttattt gctcgaacat
     1921 cggattgaac ctggtaaccg ctttactcac ggaatctagc ggttgatcgc tgtgcgaaat
     1981 agctatgcct attggtgcct gttggaaaac cgt
//
LOCUS       DAOOLY010000359         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_70878_length_2009_cov_5.225179, whole genome shotgun sequence.
ACCESSION   DAOOLY010000359 DAOOLY010000000
VERSION     DAOOLY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70878_length_2009_cov_5.225179"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLD15_12145"
     CDS             <1..409
                     /locus_tag="PLD15_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPB64711.1"
                     /translation="FDMVIPETFVADSIERLKIYRRIAACREREEIDEIEAELKDRFG
                     KLPVQVESLLTASRVRLGAFKLAIKTVEYDAHSERVVLTHAENHDRNSIRLKREKVIV
                     NDREGKTMIYPVPERRLMRVLQEIFLGAEEIVH"
     gene            384..1712
                     /gene="mnmE"
                     /locus_tag="PLD15_12150"
     CDS             384..1712
                     /gene="mnmE"
                     /locus_tag="PLD15_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPB64712.1"
                     /translation="MELKRLFTDPICALATARGVSATATVRCSGERILERLLPLLERL
                     EKPKPRKIYLTPFKVKGEFLDEILFVYFQAPASYTGEEMAELSFHGNPIIIDRAMEAL
                     LEAGFREALPGEFTRRAVLNGKIDLPKAEAINAMITSRSETALDVARRSYLEGISTEI
                     STFREEIVRLLATIEVELNYPDEVETDYTDLGEKLDGLSESISAFIESASKGLRIAQG
                     IKTVIVGETNVGKSTLLNALLRRDRAIVSDIPGTTRDTIEEDLNIKGVLFRVVDTAGI
                     RASHDAIEEMGIQRSLKAIEEADLLILLSDPAGDPGYLQKIDLEGRRVIRAANKSDIR
                     QIDGAGYDVVISARTGRGLTSLEELMVERTLDLTSIGEQIIVSARQRVCLQECLRYLL
                     QSIEAIEERVTVDIVSTLVERAAKSLDELLGRNLTEDLLDRIFSDFCVGK"
     gene            1709..>2009
                     /locus_tag="PLD15_12155"
     CDS             1709..>2009
                     /locus_tag="PLD15_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64713.1"
                     /translation="MIALLSMAIIVVAASTRLFLGPLLYFLTCVPAFVLFTVLRRKAA
                     YISLVIAPLLPIIVESIALLTGNFRGGFLVGDIVCLVTALLGLGEERPKERMIRRL"
BASE COUNT          478 a          533 c          614 g          384 t
ORIGIN      
        1 cttcgacatg gtcattcccg aaaccttcgt cgccgactcc atcgaaaggc tgaagatata
       61 ccgcaggata gcggcctgca gggaacggga agagatcgac gaaatagagg ccgagttgaa
      121 agacagattc gggaagctcc ccgtacaggt ggaatcgctt ctgacggcct ctcgtgtaag
      181 actgggagcc ttcaagctgg ccataaagac cgtggagtac gacgcgcatt ccgagagagt
      241 cgtgttgact cacgccgaga accacgatcg caactcgata cggttgaaga gagagaaagt
      301 gatcgtgaac gatagggaag gaaagacgat gatatatccg gtacccgaga gaagattgat
      361 gagagttctt caggagatat tccttggagc tgaagagatt gttcactgat ccgatatgcg
      421 ccctggcgac tgccagaggc gtttccgcga ccgcgacggt caggtgttcc ggcgagcgga
      481 tactggagag gctccttcct ctcctggagc ggcttgaaaa accaaaaccc agaaagatct
      541 atctgacgcc cttcaaggtc aagggggagt tcctcgacga gatactcttc gtgtatttcc
      601 aggcgccggc ctcctacaca ggcgaagaga tggcggaact ctccttccac ggcaatccga
      661 ttatcatcga cagggcgatg gaagctcttc tcgaggccgg tttcagggaa gctctacccg
      721 gagagttcac caggagggcc gttttgaacg gcaagatcga tctcccgaag gccgaggcta
      781 taaacgcgat gataacctcg cgaagcgaga cggctctgga cgtggccaga aggagctacc
      841 tggagggcat ctccacagag atttcgacct tcagagagga gatcgtccgg ttgctggcga
      901 ccatagaggt cgagctcaac taccccgacg aggtcgagac cgattacacg gacctgggcg
      961 aaaagctgga cggcctgtcg gaaagtattt cggccttcat cgagagcgcc agcaaggggt
     1021 tgaggatcgc tcagggaatc aagacggtga tcgtgggcga gaccaacgtc ggaaaatcca
     1081 cccttctcaa cgcgctgctc aggagggacc gggcgatcgt ttccgatatt cccgggacca
     1141 ccagggacac aatcgaagag gatctgaaca taaaaggcgt tctcttcaga gtcgtcgaca
     1201 cggccggaat acgggcctcg cacgatgcga tcgaagagat gggcatacag aggagcctga
     1261 aggccatcga agaggccgac ctcctgatac ttctcagcga tccggccggc gatcccggtt
     1321 atcttcagaa aatcgacctg gaggggagga gagtgatcag agccgcgaac aagtccgaca
     1381 taaggcagat cgacggcgcc ggttacgacg tcgtaatcag cgccaggacg ggacggggcc
     1441 tcacatcgct cgaggagctg atggtcgaaa ggaccctcga cctgacatcg atcggagagc
     1501 agatcatcgt cagcgccagg cagagggtct gtctccagga gtgtctgcgg tacctcctcc
     1561 agtcgataga agccatagaa gagagggtga ctgtcgacat agtgagcacg ctcgtcgaga
     1621 gggccgccaa gagcctcgac gaacttctgg gaaggaatct gaccgaggat ctgctcgaca
     1681 ggatcttcag cgatttctgc gtcgggaagt gattgccttg ctctcgatgg cgattatcgt
     1741 agtcgcggcc tccaccaggc tctttctcgg tccgctcctc tacttcctga cgtgtgtccc
     1801 cgccttcgtt ctcttcaccg tcctcagacg gaaagcggct tatatatcgc tggtgatcgc
     1861 ccctctgttg ccgatcatcg tggaatcgat cgccctgttg acgggcaact tcaggggggg
     1921 gtttctcgtt ggcgacatag tctgcctggt caccgcgctg ctgggactgg gcgaagaaag
     1981 gcccaaagag aggatgataa ggcggctgg
//
LOCUS       DAOOLY010000360         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_72093_length_1985_cov_3.681347, whole genome shotgun sequence.
ACCESSION   DAOOLY010000360 DAOOLY010000000
VERSION     DAOOLY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72093_length_1985_cov_3.681347"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1271
                     /locus_tag="PLD15_12160"
     CDS             15..1271
                     /locus_tag="PLD15_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64714.1"
                     /translation="MKKLVGILVVLLAISVAFSATITLSPSIKGTLWGKTQLDKTGLT
                     TDLGFTLKSLGFGASADVSDLTFSLTIDLVGGTVTLNSFTVENDKAAASWYASKSFGS
                     DSGKGLGWFGYYGTTKSKTFVLNMKALGLQVATQEALLGGTDRIALYGSWDIFSMALQ
                     TGMAGLGWSGDLIAEATIKPFTGLTLKGGLEAKDLTGTPTFDYVIDFDYKMTLGLLTL
                     NPYARYASTKGQWVGLKVGYGIGESVVLKINADVKYDIAASKLFSWIQVALSKKGIGW
                     AGVKFWYDHSFLVGGTNEMKLGFLVKSDGWEIDPVSLAVFVGSGDFTTKNDNNKSGFG
                     YDIVGNLLADVKDLSAYAEASLSLAMGGFKPTLSIDGGYYLLNGTKELNVVLGFPVFD
                     LINFEASVAFFPAIDWYVKLFYNYSF"
     gene            1329..1817
                     /locus_tag="PLD15_12165"
     CDS             1329..1817
                     /locus_tag="PLD15_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64715.1"
                     /translation="MYIFLGNLLMGVAIVIALVFGGGQFVTALLNGESPELDWMDFLF
                     PAALLLTGFVLQFCSIAKHYFIPVIEELFLAGSLFLYVRESLEGGLTWWLVPLREYIS
                     STLLYTATGSLLPVILVVLMASLVVKMLNMSALKTGNDEPRREREDASNKNGTLRSRR
                     SL"
BASE COUNT          450 a          522 c          522 g          491 t
ORIGIN      
        1 gggggtaagt gtgcatgaag aagcttgttg gaatcctagt ggtactcctg gccatatcgg
       61 tcgccttctc ggcaaccata actctcagcc catcaataaa agggacgtta tggggcaaga
      121 ctcagcttga caagaccggc ctgaccactg atcttggttt tactttgaag tctctaggct
      181 ttggagcctc tgcagatgtc agtgacctga cattcagcct tactatcgat ctagtgggcg
      241 gtactgtaac tctcaattcc ttcactgtgg agaacgacaa ggcggctgcc agctggtacg
      301 ccagtaagtc ctttggcagt gacagcggca aaggcctcgg ttggttcggc tactacggta
      361 ctaccaagtc taagaccttc gttctcaaca tgaaggcgct cggtctccag gtggcaacgc
      421 aggaagccct cctcggcggt acggacagaa tcgccctgta cggctcctgg gatatcttct
      481 ccatggccct tcagacaggt atggccggtc tgggctggag cggtgacctc atagccgaag
      541 ctaccattaa gccgttcacc ggtctcacgc tcaagggcgg tctcgaggcc aaggatctca
      601 ccggtacacc gacgttcgac tacgttatag atttcgatta caagatgacc ctcggacttc
      661 tcactctgaa cccgtatgcc agatacgcca gcaccaaggg ccagtgggta ggtctcaagg
      721 ttggttacgg tatcggtgaa agcgtcgttc tgaaaatcaa cgccgacgtt aagtacgata
      781 tcgccgccag caaactcttc agctggatac aggtcgctct ctccaagaag ggcataggct
      841 gggcaggcgt gaagttctgg tacgaccact ccttcctggt gggcggaacg aatgaaatga
      901 agctcggatt cctggtcaag tccgacggct gggagataga tccagtttct ctggctgtct
      961 ttgtcggttc cggcgacttc acgaccaaga acgataacaa caaatccggc ttcggttacg
     1021 atatagtcgg caacctactc gccgatgtga aggatctctc cgcttacgca gaggcttccc
     1081 tctctctcgc tatgggtgga ttcaagccga cactcagcat cgacggcggt tactacctgc
     1141 tcaacggcac gaaggaactc aacgttgtgc tcggcttccc ggtcttcgat ctgataaact
     1201 tcgaagccag cgttgccttc ttcccggcga tcgactggta cgtgaagctg ttctacaact
     1261 actcgttctg attttaacgc taacgatgaa ccggcccgac gggccggttt ttctttgagg
     1321 tgaattagat gtatattttc ctcggtaacc tgctaatggg agtcgcgata gtgatagccc
     1381 tggtctttgg aggcggtcag ttcgttacgg ccttgctgaa cggagagagt cccgaactgg
     1441 actggatgga ttttctcttt cccgctgcgc tcttattgac gggctttgtc ctgcaattct
     1501 gctcgatagc gaaacattac ttcatcccgg taatcgaaga actctttctt gcaggctctc
     1561 tatttcttta cgtgagggaa agtctggaag gcggtctaac ctggtggctg gttcctctaa
     1621 gagagtacat aagctcaaca cttctgtaca ccgctacagg ctctctactg ccggttatac
     1681 tcgttgtcct gatggcctcg ctggtggtga agatgctcaa catgtctgcc ctgaagacag
     1741 gaaacgatga accacgcagg gaaagggaag acgcctcgaa caaaaacggg acccttaggt
     1801 cccgtcgaag tctgtagttg agttaattac ttattagaaa cccagtgatc ttggatctag
     1861 cgcatccctc agggcatcac ccagcaggtt gaaggccata accacgatga atatgaagat
     1921 acctggtatc agaagccacg gtgcctcggt catcgccttt atgttctgag cctgagagag
     1981 cataa
//
LOCUS       DAOOLY010000361         1971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_72843_length_1971_cov_2.442067, whole genome shotgun sequence.
ACCESSION   DAOOLY010000361 DAOOLY010000000
VERSION     DAOOLY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72843_length_1971_cov_2.442067"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1919
                     /locus_tag="PLD15_12170"
     CDS             <1..1919
                     /locus_tag="PLD15_12170"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB64716.1"
                     /translation="NIPDLIIDEGAVLTIDLHDYSSDEKKSLLVFQKISGPGEIKGNL
                     YTFSPTFQQSGKHSVSITASDQSDQIAEETFIIEVREVNRQPTFALPSLTLGKGEMVS
                     INLDDYAKDPDLDTLIYSIEMGGGQIIGSSYTYKPSFSNSREETVSIRVSDGRGGIAT
                     SVLKLSDRDFIASIDQQSQRATDTTAQGTAATGTDTQVQTPIATNNPPVVSVPDQRMN
                     QGGTLAIDLKGFVIDKDNDAITIRKVSGPGTLERTLYSYSSPPNDSGQKVVELEVSDG
                     KTTVRSSFKIDVIAQNRSPIAVDIPQNSVVQGQTFSLNLNNYFSDPDGDPLSYQILSG
                     PGNIQNGFFTFQSQTTGLFSTTIRASDGKGGTVDRTVTVNVQASNRPPVISITPRRIA
                     EGEVLTIDLAASSSDPDNDRLSYTLVSGPGTVQGSVYTLRTDFNSAGNYSVVIRVDDG
                     KGGNTTGSFAVEVRDVNRPPLLELSGITVPEGSTYVVDLSKVSSDPDGDALTYSIVQG
                     IGRIENNNYIVEPDYDDYGFYTVVIRASDGKGGQVSSTFDLLVADTNRAPLFTIPDQT
                     TMSTRTLRLDLREFSSDPDGDYLRYELVYGPGLLTGSIYSFTPEGLGTSTVMLKASDL
                     KGGETTTTFTITVR"
BASE COUNT          557 a          474 c          497 g          443 t
ORIGIN      
        1 tgaacattcc cgatctgata atcgatgagg gagccgtcct gaccatcgat ttgcacgatt
       61 attcgagtga tgagaaaaag agtttgctcg tcttccagaa gatctccgga cccggcgaga
      121 taaaaggtaa tctatacacg ttttctccaa cctttcagca atctggtaag cattcggtga
      181 gcatcacagc cagtgaccag agcgatcaaa tcgcagaaga aactttcatc atcgaagtca
      241 gggaagttaa ccgtcagccg acctttgcct tgccgtcgct aactttagga aaaggcgaga
      301 tggtttccat aaacctggat gattatgcga aagatccaga cctagatact ttgatttact
      361 cgatagaaat gggtggcggt cagataattg gcagcagtta tacctataaa ccgtcttttt
      421 caaattccag agaagaaact gtcagcataa gggtgtccga cggaagggga ggaattgcaa
      481 catcggtact caaactctcc gatagggatt tcatcgcttc cattgatcaa caatcgcagc
      541 gtgccaccga taccacagcg caggggacag ctgcaaccgg aacggatacg caggtacaga
      601 caccgatagc caccaacaat ccgccggtgg tatctgttcc agaccagagg atgaaccagg
      661 gtggaactct tgcgatagat ctgaagggct ttgtcatcga caaagacaac gatgcgatta
      721 cgataaggaa agtatcggga ccgggaacgc tggagcgaac actgtactca tattcgagtc
      781 cacccaacga tagcggtcag aaggtggtag agcttgaagt ctccgacgga aagaccacag
      841 tgcgatccag cttcaaaatt gatgtgatag ctcagaacag gtcgccaatc gccgtcgata
      901 ttccccagaa ctccgtagta cagggccaga ctttttcact caatctcaac aactatttca
      961 gcgaccccga tggcgatccg ctgtcgtacc agatcctgag tggaccggga aacattcaaa
     1021 atggtttttt cactttccaa tcgcagacca caggcctctt ctcaactacc atcagggctt
     1081 ccgatggtaa gggaggaact gttgacagaa ctgtaacagt caatgttcag gcttccaata
     1141 gacctcccgt tatctcgata actcctagaa gaatcgccga aggagaagtc ctgacgatcg
     1201 accttgcggc cagttcttca gatccagaca acgacaggtt gagctacacc cttgtatcgg
     1261 ggccgggaac tgttcagggt agcgtctata cgcttaggac ggacttcaac tcggccggaa
     1321 actactcggt ggttatcagg gtcgacgatg gaaagggagg taacacaact ggctcctttg
     1381 ccgtagaagt cagggatgtc aacagaccac cgttgctgga actctcaggt ataaccgttc
     1441 cggaaggttc aacgtatgta gtcgatctct cgaaggtttc ttcggatcca gacggcgacg
     1501 ctcttaccta ctctatagtg cagggaattg gaaggataga gaacaacaat tacattgtgg
     1561 agccagacta cgacgactat ggtttctata cagtggtgat aagggcttca gacggcaaag
     1621 ggggtcaggt ttcatctact ttcgatcttc tagtagcaga cactaacagg gcaccactat
     1681 tcacgatacc tgatcagaca acgatgtcca ccagaacatt gaggctggat ctcagggagt
     1741 tcagcagcga cccggatgga gattatctca ggtacgaact ggtttacggg ccgggtttgt
     1801 tgaccggaag catttactcc ttcactcccg agggactcgg cacaagtacg gtcatgttga
     1861 aggccagcga cctgaagggc ggcgaaacga ctaccacatt cacaattacg gtcaggtaat
     1921 tgaggattga aatgagatat cgatcaaaaa atgcgattgc gcaaatggaa a
//
LOCUS       DAOOLY010000362         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_72884_length_1970_cov_3.384334, whole genome shotgun sequence.
ACCESSION   DAOOLY010000362 DAOOLY010000000
VERSION     DAOOLY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72884_length_1970_cov_3.384334"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLD15_12175"
     CDS             complement(<1..369)
                     /locus_tag="PLD15_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64717.1"
                     /translation="MLEVKNLNVSYGRIQVIKDFTFSVGDEAIGFFGPNGAGKTTLIN
                     SIIGLVRPTSGEINFEGQSLLDRETHQIVRMGISVVPQDGELFPIMTVIENLRIGSAY
                     IKVAREKTSERLEFVFKIFPI"
     gene            complement(369..1085)
                     /locus_tag="PLD15_12180"
     CDS             complement(369..1085)
                     /locus_tag="PLD15_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64718.1"
                     /translation="MSILKVDRVTKRFGGLVAVNDVTMEIQKGEILGLIGPNGAGKTT
                     LTNLISGVHLVSEGKIVFEDTDITLIPAHKRCKLGIARTFQIARPLKNLTVVENIMIG
                     GLFGKGENLKTGRKSALELCKGIELKKPDETLDRITVLDIKKMEIARAMATNPKILFL
                     DEVMSGLNMDETRDMIHTVRKIRDTGVTICIIEHVMSVIRELTERVIVLDRGEIIAEG
                     PYSEVSMQHNVISAYLGEDA"
     gene            complement(1087..>1970)
                     /locus_tag="PLD15_12185"
     CDS             complement(1087..>1970)
                     /locus_tag="PLD15_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPB64719.1"
                     /translation="PIVTEAYFISIAITILTFMTLALSWDMMLRTGQLSFGTAGFFGL
                     GGYTGIIAAADFGVDPVIGIMLGGLVAGGIALILGMAILRLRGLYFAITTMALASVFM
                     VIIRNLHRLTGGPSGKIVYNTLFNYDSNKVYWFMLAIAVGTVVISELFQRTRIHFAID
                     SIRADETVAKSHGVNIYKYLIFVFVVTSIIQGVIGATYALQYGFVYPESSFSPHYLLL
                     PIAMALVGGIYSTIGPILGAIILGLSSEYLKLIMPYGHLVIYGVIIVVVVLFMPDGVY
                     GAIKAFFMNRKRRLEER"
BASE COUNT          490 a          506 c          429 g          545 t
ORIGIN      
        1 aataggaaag atcttgaaca caaactcaag cctctcggac gtcttttccc tggctacttt
       61 tatgtatgcc gatcctattc ttagattctc tataactgtc attatcggga agagttctcc
      121 atcctgtgga acaacggaga tccccattct cactatctgg tgagtctctc gatcgagcag
      181 cgactgccct tcgaagttta tctctccgct agtcggtctc accaatccta tgatcgaatt
      241 tatgagtgtg gtcttgccag cgccgttcgg tccaaaaaag ccgatggctt cgtcaccgac
      301 ggagaaggtg aagtccttga tcacctgaat tcttccatat gaaacgttca agttttttac
      361 ttccagcatc aggcatcctc ccccagatag gcactaataa cgttgtgctg catcgacact
      421 tcgctgtaag gcccctcggc tatgatttct cctctgtcga gcacgatgac tctctctgtc
      481 aattctctta tcacgctcat tacatgttcg attatgcaga ttgtgactcc cgtatctctg
      541 atctttctga cggtgtgtat catatctctc gtttcatcca tgttcagtcc ggacataact
      601 tcgtccagga agaggatctt tggattggtt gccatagctc tggctatctc catttttttg
      661 atatccagaa cggtaatcct atcaagagtt tcgtcgggtt tttttagctc tatcccttta
      721 cagagctcta aagcgctttt acgaccagtt ttgagattct cacctttacc gaagaggcct
      781 ccgatcatta tgttctccac aacggtgagg ttcttcaaag gtctggcgat ctgaaaggtt
      841 ctggcgatgc ccagcttgca tctcttgtgg gcggggataa gagttatgtc cgtatcctcg
      901 aaaacgatct ttccttcact caccaggtgt acgccggaaa tcaagttggt caacgtagtt
      961 ttaccggcac cgttcggtcc tatgaggcca agaatctcgc ccttttgaat ctccattgtg
     1021 acgtcgttga cggccacaag tcctccaaaa cgtttggtta ctctatctac cttcagtatg
     1081 ctcatcttat cgttcctcca gacggcgctt tctattcatg aaaaaagcct tgatagcacc
     1141 ataaactccg tcgggcatga aaagaacaac tacgactatt atcacaccgt atatcaccag
     1201 atgaccgtaa ggcattatca attttagata ctcggaggat aggccgagga ttatggctcc
     1261 cagtatcggc ccgatagttg agtatatacc ccccacaaga gccatggcga taggcagaag
     1321 caagtaatgt ggcgagaagc tgctttcggg atatacaaac ccgtattgaa gggcgtacgt
     1381 ggcccctatc acaccctgaa tgatcgatgt caccacgaaa acgaagatga gatatttgta
     1441 tatattcact ccgtgcgact tggcgacggt ttcgtcagcc ctgatcgagt cgatggcgaa
     1501 gtgaattcta gtcctctgga acagctccga tatcactacg gtaccaaccg ctatggcgag
     1561 cataaaccag taaactttgt tcgaatcgta attgaataga gtgttgtaaa ctatctttcc
     1621 gcttggacca ccggttagtc tatgaaggtt tcttattatc accatgaaga cgcttgcaag
     1681 cgccatggtg gtgatggcga agtacagacc tctcaatctc aggatggcca tacccagtat
     1741 aagagctata cctccggcaa cgagtccgcc cagcatgatg ccaatcaccg gatcgactcc
     1801 aaaatctgcg gccgctatga tacctgtata accaccgagg ccgaaaaaac ctgccgtgcc
     1861 gaacgatagc tgacccgttc tcaacatcat gtcccagctg agagcaagcg tcatgaaagt
     1921 gagaatagtg attgcgatgc tgatgaagta cgcctcggtc acgattggaa
//
LOCUS       DAOOLY010000363         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_73049_length_1967_cov_3.928870, whole genome shotgun sequence.
ACCESSION   DAOOLY010000363 DAOOLY010000000
VERSION     DAOOLY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73049_length_1967_cov_3.928870"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLD15_12190"
     CDS             <1..265
                     /locus_tag="PLD15_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB64720.1"
                     /translation="KGMITNSYERETTLRLQTSIDGKPKYSQENPAYFDPYTEELKSF
                     ILSIEEDREVSVNGQEAIESLRVALAANLSAELLRPVELSEVF"
     gene            267..1238
                     /locus_tag="PLD15_12195"
     CDS             267..1238
                     /locus_tag="PLD15_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB64721.1"
                     /translation="MTKIAILGIAHMHGYSYARALKGMNDVGIAGLYDEDRSRMNRAC
                     EDLGIKPCEHPEELVAGSDGVIVTSENSTHRKFVEIAAGAGKHVLCEKPIATTVEDAE
                     AMIEVCRRNNVKLQMAFPVRYSASVRKAREIISGGLLGEVVSLVGTNHGRMPGGWFID
                     PVLGGGGAVMDHTVHVVDIVRWLLNCEFTQVYATYGTMIHDIPVEDCGLEMFKLSTGQ
                     YMTLDCSWSRPKAHPYWGDVTLHIVGTEGTLFLDVFNSKIEVYSNEKGGRWDNYGDNL
                     DSLMIEEFLKVLREGKEPFTDGNDGLQSLKAVTAAYKSFREGKVVEI"
     gene            complement(1342..>1967)
                     /locus_tag="PLD15_12200"
     CDS             complement(1342..>1967)
                     /locus_tag="PLD15_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB64722.1"
                     /translation="IVEPSVLLLDEPLSNLDALLREQMRLEIKRIQKSLGITTIYVTH
                     DRVEAMSLSDRIIVMKNGFVRQIGSPDRIYEDPDSKFVAGFVGKVAFLPVEITAVDGR
                     CSVRFEDRVFEVGKFSPELKPGDRGVIMARPESLKLLEPGAGMIEGRVNTNIYLGGSL
                     ESFVSTDRGEILVQIDNPAEKKVFAEGEGVSIGIIPNLVKALPDRDD"
BASE COUNT          459 a          504 c          581 g          423 t
ORIGIN      
        1 taagggaatg ataaccaaca gctacgagag agagacgacg cttcgtctgc aaacctcgat
       61 cgacggaaag ccgaaatact cgcaggagaa tccggcctat ttcgatccgt acaccgaaga
      121 attgaagtct ttcatactct cgattgagga agacagggaa gtctccgtta acgggcaaga
      181 ggccatagaa tcgttgagag tcgcgctggc ggccaacctg tcggcagaac tgctcagacc
      241 ggtcgaactt tcggaggtgt tttgagatga cgaaaatcgc gatcctcggt attgctcata
      301 tgcacggtta cagctatgcg agagccttga agggaatgaa cgatgtcggg atagccgggc
      361 tttacgacga ggacaggtcc aggatgaaca gggcctgcga agatctcgga attaagccct
      421 gcgaacatcc cgaagaactc gtcgccggaa gcgacggcgt gatagtgacg agcgagaact
      481 cgacgcacag gaagttcgta gagatcgctg ccggagcggg aaaacacgtt ctctgtgaaa
      541 agccgatcgc gacgaccgtg gaagacgcgg aggcgatgat cgaggtctgc cgcaggaaca
      601 acgtgaagct ccaaatggcc ttcccggtga ggtacagcgc ttcggtgaga aaggccagag
      661 agatcatctc gggaggtctg ctgggcgagg tggttagtct ggtgggaacc aaccacggga
      721 gaatgccggg cggctggttc atagaccccg ttctgggcgg tggcggcgcc gttatggacc
      781 acaccgtgca cgtggtggat atagtgaggt ggcttctgaa ttgcgagttc acacaggttt
      841 acgccactta cggcaccatg atacacgaca taccggtcga agattgcggg ctggagatgt
      901 tcaaactctc gacgggccag tacatgacgc tcgattgcag ctggtcgaga ccgaaggccc
      961 atccttactg gggagacgta actcttcaca tagtcggaac tgagggaacg ctctttctgg
     1021 atgtcttcaa ttcgaagatc gaggtctatt ccaacgaaaa gggcggccga tgggacaact
     1081 acggcgacaa tctcgattcg ctcatgatag aggagttcct gaaggtcctg agggagggca
     1141 aagagccttt cactgacgga aacgacggcc tgcagtcgct caaagccgta acggccgcgt
     1201 acaagagctt cagagagggc aaagtagtgg aaatctgaag agcaggaaag aggaggagag
     1261 agctctttct ctcgtgtttt tcgctctcgc tttctcgtga gcggagcgaa ctctcgatgc
     1321 tttcgtcttc cataggtacc gttaatcgtc tctgtcgggc agggccttga ccaaattggg
     1381 tatgatccct atcgagactc cttcgccctc ggcgaagacc tttttctcgg cggggttgtc
     1441 tatctgcaca agaatctcgc cgcggtcggt cgatacgaag gactcaagac tgccgccgag
     1501 atagatgttc gtgttgactc tgccttcgat catgccggcg ccgggttcga gaagtttgag
     1561 cgattccggc ctggccatta ttacgcctct atcgcccggt ttcagctccg gagagaactt
     1621 tccgacttcg aaaaccctgt cttcgaatct cacgctgcac ctgccgtcga cggccgttat
     1681 ctcgaccggg aggaaggcca ctttgcccac gaaaccggcc acgaacttgg agtcggggtc
     1741 ttcgtagatc ctgtctggcg agcctatctg tcttacgaag ccgtttttca tcactataat
     1801 cctgtctgaa aggctcatcg cctcgaccct gtcgtgggtg acgtagatcg tggttatccc
     1861 cagagacttc tgaatccgtt ttatctcgag cctcatctgc tccctgagaa gcgcgtctag
     1921 atttgagagc ggctcgtcca gcaggagaac ggaaggctcg acgatca
//
LOCUS       DAOOLY010000364         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_73214_length_1964_cov_4.403876, whole genome shotgun sequence.
ACCESSION   DAOOLY010000364 DAOOLY010000000
VERSION     DAOOLY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73214_length_1964_cov_4.403876"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLD15_12205"
     CDS             <1..355
                     /locus_tag="PLD15_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64723.1"
                     /translation="IFGYEVYKNGPDFETALEKAGVMAKGLVKKEIDDLLARSINYGY
                     ALKAVAFGMERYSQIKKYIEANFGSIHDSTLSNILNGLVKQSFLEISFSKSIKSYSIP
                     DPITRSFCVSMNLKQ"
     gene            629..1006
                     /locus_tag="PLD15_12210"
     CDS             629..1006
                     /locus_tag="PLD15_12210"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HPB64724.1"
                     /translation="MKGGNCIIRLIETDKGKVGIMERRDEVFRSSQDVLDAIADLCYQ
                     ECDRFIFYKENFPEDFYDLRTGLAGDILQKMSTYYIKLAIVGDFSGYTSKSLKAFIYE
                     SNRGKQNFFVASEEEAMKALGLS"
     gene            1134..1964
                     /locus_tag="PLD15_12215"
     CDS             1134..1964
                     /locus_tag="PLD15_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64725.1"
                     /translation="MTILKVRVLVAAFITLAVFSFAGLLSGPADRFRLELDYESGFTG
                     VIFHTVQFGQGGTVFNYVKDGGQDVLFPFERFTASVGIDRHTVYFLYQPLTVQTKVKL
                     KSDLLVDDVLFPAGTGMVLNYGFPFYRLSYTYDVLRKGDSYLALGLSLQIRNADIYFE
                     SLDGTKFVANKNVGPVPVIKIKGELWFNNRVYIATDIDGFYASSSFFNGADFDFEGSV
                     LDASLRGGLKLTDYLEAYLNLRFIGGTAKGVSKNADSPDGFTDNRLATINVSIRFQLK
                     "
BASE COUNT          515 a          460 c          505 g          484 t
ORIGIN      
        1 aattttcgga tatgaagtct acaagaatgg acctgatttc gagacggctc tcgaaaaggc
       61 aggagtcatg gcaaaaggac tggtaaaaaa ggaaatcgac gatttgctcg ccagatcaat
      121 aaactacggc tatgccctaa aagcggtagc gttcggcatg gagaggtatt ctcagataaa
      181 aaaatacata gaagcgaact tcgggtctat tcacgactct acactcagca atattttgaa
      241 cggacttgta aaacaaagtt tcctcgagat ctccttctca aaatcgataa agagctactc
      301 tatacccgat ccgataactc gcagcttctg cgtttcgatg aacttgaaac agtaacaacc
      361 atctcaacca acacactttt gaatttataa gagcactacg ggatgacggt gcgagggcat
      421 tcacgggatg acacgcgaaa aacgggacag acgtgaggag aatcgtcagt ccctattttc
      481 gttcccttcc cgtcatgccg gaccccgatc cggcatcccg ctcttaccca caatggttct
      541 taataggagc atttcagtgc aggctctgca ggatgagacg aagagagcgt ttcccgggca
      601 gtgtctttct gttatcatat atcgatgaat gaaagggggg aattgtatca taaggcttat
      661 cgaaactgac aagggcaagg ttggtattat ggagagacgc gacgaggttt tcaggagttc
      721 gcaggacgtt ctcgatgcga tagccgatct gtgttatcag gagtgtgata gattcatttt
      781 ctataaagag aacttcccgg aggatttcta tgatctccgg acgggcctgg ccggagacat
      841 actgcaaaag atgtccacct attatataaa actggcgatt gtgggcgact tcagcggcta
      901 tacgagcaaa agccttaagg ccttcattta cgaatcaaac aggggaaagc aaaacttctt
      961 cgtcgcgagc gaagaagagg ctatgaaagc cctcggtctt tcttaaggcg gctttttcgc
     1021 tttcccgcaa atccttgcag gagctttcat cattttaaca gagatgccaa aacagcttga
     1081 cagtatccga catgccgttt gtatgaagga aaaccatata atcgtagctg gaggtgacta
     1141 ttttgaaagt cagagtattg gtcgcggcat ttataacgtt ggcagtgttc tcgttcgcag
     1201 ggttgttgag cggtccggct gatcgttttc gccttgagct ggattacgag tcgggcttca
     1261 ccggcgtgat tttccacacc gttcagttcg gccagggcgg tacggtattc aattacgtga
     1321 aagacggagg ccaggacgtc ctcttcccct tcgagagatt caccgccagc gtagggatcg
     1381 acaggcacac cgtatatttt ctctatcaac cactgacggt gcagactaag gttaagctga
     1441 agagcgatct gctggtggac gacgttctct ttcccgcggg caccgggatg gttttgaatt
     1501 acggctttcc cttctatcgt ttgagttaca cgtacgacgt cttgagaaag ggcgacagct
     1561 atctggcgct ggggctttcg cttcagatta gaaacgccga tatatacttc gaatcgctcg
     1621 acgggacgaa gttcgtggcc aacaaaaacg tcggccccgt tccggtaatc aagataaagg
     1681 gcgagctctg gttcaacaac agggtctaca tcgcaacgga catagacggt ttctacgcct
     1741 ccagcagttt cttcaacggg gccgacttcg acttcgaagg ctcggtgctt gacgcttctc
     1801 tgcgcggcgg gctgaagctt acggattatc tggaagccta cctgaatctg cgcttcatag
     1861 gcggcacggc aaagggcgta tcgaagaatg cggacagccc cgacggcttc accgacaata
     1921 gactggcaac tataaatgtg agtatccgtt tccagctcaa gtag
//
LOCUS       DAOOLY010000365         1955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_73717_length_1955_cov_4.293684, whole genome shotgun sequence.
ACCESSION   DAOOLY010000365 DAOOLY010000000
VERSION     DAOOLY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73717_length_1955_cov_4.293684"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PLD15_12220"
     CDS             <1..1015
                     /locus_tag="PLD15_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPB64726.1"
                     /translation="VLFTPFVLLVAFRKYGREHEVGYFGDYERELPTDDAADVVNAAV
                     KNMAGVVDNDGIGSVMMELYRKNYIDFERGKDDKVDGILIKNRDSSDLRPTEAAFLKF
                     LDKYDENDLFDFSDVKKKATKSQSKARTFTSDLSVYKDKVNNTVKSKKLLDTRGNSMA
                     KGYSTLMLLLSILMLMVHSNPAISHLRLFSLVFYGALWTTSFVVFMMPRDVFGRWTPE
                     GRVFYLKWMGLKRYLEDYSLLNEKPPESIILWEEYLVYATALGIADTVRKNLNRIVPE
                     DIWREQSRHPFIYYPVTAYAINSFNTLTTSVRAASSSGSGGGGFGGGGSGGGGGGGGT
                     GGF"
     gene            complement(1080..>1955)
                     /locus_tag="PLD15_12225"
     CDS             complement(1080..>1955)
                     /locus_tag="PLD15_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPB64727.1"
                     /translation="YWSAGVYGIAIAALGMLSFVGYIVSVDSYGPVADNAGGIAQMAN
                     LDPKVRKLTDRLDSVGNTTAAIGKGFAIGSAAFAALALIVSYIWGAADTANEIVNNPI
                     INLVEPYTLIGILIGAMLPFFFTSLLIKGVSDTANLMIVEIRRQFKENPKIRTGDAVP
                     DYKRCIEITTKGAVSRMLTPGVVAIASPFVIGLLFGRSAVAGLLIGGLASAIMIALFT
                     ANSGGAWDNAKKYIETGEFGGKGTPTHEAAVVGDTVGDPLKDTVGPSMDILIKLMSVV
                     SLVFGSIFPAGPFFM"
BASE COUNT          493 a          483 c          518 g          461 t
ORIGIN      
        1 agtgttgttc acaccttttg tattgctcgt agcgtttcga aagtacggca gggaacacga
       61 ggtgggatac ttcggtgatt acgagcgcga attaccgacc gacgacgctg ccgatgttgt
      121 aaacgcggcg gtgaagaata tggccggagt ggtagacaac gacggtattg gttcggtgat
      181 gatggagctg tacaggaaga attacataga cttcgaaagg ggaaaggatg acaaagtcga
      241 tggaatactc ataaagaaca gggattcttc agatctgagg ccgacagagg ctgctttctt
      301 gaagttcctc gacaagtacg acgaaaacga tctcttcgac ttttcagatg tcaagaaaaa
      361 agctacaaaa agccagtcca aagccagaac cttcacttct gatctaagcg tttacaaaga
      421 caaggtgaac aacacggtga aatccaaaaa actccttgac acaaggggca actctatggc
      481 caaaggctat tctacattga tgcttctact ttcgatattg atgctcatgg tacattcgaa
      541 cccggcgatc tctcatctgc gcctcttttc gcttgtattc tacggagcac tgtggactac
      601 cagtttcgtt gtcttcatga tgcccagaga cgtttttggt cgctggacgc ccgagggaag
      661 agttttctat ctcaaatgga tgggtcttaa gagatatctt gaggattact ccctgctaaa
      721 cgagaaacca cccgaatcga taatcctctg ggaagagtat ctggtctatg ctacggctct
      781 gggtatagcc gataccgtga gaaagaatct aaacaggata gttcccgaag acatctggcg
      841 cgagcagagt cgccatccct tcatctatta cccggtgacg gcttatgcaa tcaactcttt
      901 caacactcta actacatcgg tcagggcggc ttcctcgagt ggctcgggcg gcggaggttt
      961 cggcggaggg ggctccggag gaggaggagg aggaggagga actggcggct tctgattcat
     1021 cgttcaaata aggaagatag tgcggccata gtcggccgcg ctatcttttc aaatccgtat
     1081 cacatgaaga aaggtccggc cgggaagata gagccaaaga ccagcgatac gaccgacatc
     1141 aatttgatca gtatatccat ggaggggcct acggtatctt tgagaggatc gccgacagta
     1201 tctcctacaa cggccgcctc gtgggtgggg gtacccttgc cgccgaactc tccggtctca
     1261 atgtatttct tcgcgttatc ccaggctccg ccggaattgg cggtgaagag agctatcata
     1321 atggcacttg caagcccgcc aatcagcagg ccggcaactg cagaccttcc gaacagaaga
     1381 ccgattacga acggcgatgc gatggctaca actccgggag taagcattct gctcacggct
     1441 cccttggtag ttatctcgat gcacctcttg taatctggca ctgcatctcc ggtcctgatt
     1501 ttggggttct ctttgaactg ccttctaatt tctacgatca tgagattggc ggtgtcggag
     1561 acacccttta tcaacagcga ggtgaagaag aagggaagca tagctcctat caatattcct
     1621 atcaaggtgt aaggttccac caggttgatg atcgggttgt tcacaatttc gttggccgta
     1681 tcggcggcac cccagatgta cgagacgata agcgcaaggg cggcgaaagc ggccgagccg
     1741 atggcgaatc ccttccctat ggctgctgtg gtgtttccaa ccgagtccag cctgtcggtc
     1801 agctttctaa ccttcggatc gaggttggcc atctgcgcta tgccaccggc gttatctgca
     1861 accggtccgt aggaatcgac cgaaactatg taacccacaa acgacagcat tcccagcgca
     1921 gctatggcga ttccgtaaac tccggccgac cagta
//
LOCUS       DAOOLY010000366         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_73984_length_1950_cov_4.712401, whole genome shotgun sequence.
ACCESSION   DAOOLY010000366 DAOOLY010000000
VERSION     DAOOLY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73984_length_1950_cov_4.712401"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLD15_12230"
     CDS             <1..794
                     /locus_tag="PLD15_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPB64728.1"
                     /translation="LRKSYQTGVNLTAVSMEYLISIFSMVKSDLWMPDVIVAAFSGAG
                     DQERKDKLSGALKTAFYGASIEVMMDIEGLYRAAVGSGRGVVVISGTGSTVYGHDDEG
                     TPFRSGGWGHIFDDEGSSFWMSREIITCALRYRDGLLPHDPIFDLLLEFYSVESIEKL
                     VNLTIIQDFKTKIASFSKVALEQPTPLVREIVDEGIGLLARRTLKVLEKTGPVDSISV
                     HGGSFQSAYYRERFTRALGGHKISLFEGNVDEILAKQVFDTLNRS"
     gene            complement(781..1941)
                     /locus_tag="PLD15_12235"
     CDS             complement(781..1941)
                     /locus_tag="PLD15_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPB64729.1"
                     /translation="MSELKVKLGIDGFLEREDFKGKRLGLLTNSTGINGRLKRNIDLI
                     VSSGFDLVKLFGPEHGISGAAPDGVGVGNAVDPRYGIPVYSLFGEIYRPTEEMLSDLD
                     LFVYDIQDVGLRFYTYIYSLAYSMEECARRGIKVIVLDRPNPLSCKVEGPTIKKGFET
                     FVGGYGLALRYGLTVGELARYYNAAFNMGADLEVVPMENYDRNMYFDETGHLWNTPSP
                     NIPSFEHALLYTGFCLFEGTNLSLGRGTVHPFKYIGAPWIDPELLYKEVSRLNPPGVA
                     FRERSFIPSAFRLSNRACHGLEFFVTDRKRIEPLELAIDIISTVRRVYPEEFQWDLQY
                     HGADGRYHFDLLMGESLYRKKIEEGAASKDFVAIWTDEERNFEEFIKPFRIY"
BASE COUNT          445 a          519 c          529 g          457 t
ORIGIN      
        1 agctcagaaa aagttatcaa accggagtaa atctgactgc cgtcagtatg gagtatctga
       61 tctctatctt ctcgatggtt aagagcgatc tctggatgcc cgacgtgatc gttgcggcct
      121 tttccggagc cggagaccag gaaaggaaag ataagctgag cggcgccctg aagacggcct
      181 tctacggagc ttcgatcgaa gtgatgatgg atatcgaagg actttacagg gcggccgtgg
      241 gcagcggaag gggagttgtg gtgatatccg gaaccggctc taccgtttat ggccacgacg
      301 acgaaggaac tccgtttaga tcgggcggct gggggcatat cttcgacgat gaaggaagca
      361 gtttctggat gtccagggag ataataacct gcgctttgag gtacagggat gggctgctgc
      421 cgcacgaccc gatcttcgac ctgctgctgg aattttattc tgtggaaagc atagagaaac
      481 tcgttaacct tacaataatt caggatttca aaacgaagat cgcatcgttc agcaaagtgg
      541 ctctcgagca gccaacgccc ctggtcagag agatcgtcga tgagggaatc ggtttgctcg
      601 ccagaagaac tctgaaggtt ctcgaaaaaa ccggacccgt tgactcgata agcgtccacg
      661 ggggttcttt ccagtcggcc tactatcgcg agagattcac ccgggcgtta ggaggccaca
      721 aaatctccct cttcgaggga aacgtcgacg aaatactggc aaagcaggtt ttcgacacgc
      781 tcaatagatc ctgaagggct ttataaattc ctcgaaattc cgctcttcgt cggtccagat
      841 agccacaaaa tccttcgaag cggccccctc ttcgattttt ttgcggtaga ggctctcacc
      901 catcagcagg tcgaaatgat atctgccgtc ggctccgtgg tactggagat cccactggaa
      961 ttcttccgga tacactcttc gcacggtcga tatgatgtcg atcgccagtt cgagcggttc
     1021 tatacgcttc ctgtcggtca cgaaaaactc cagcccgtgg cacgccctgt tcgagagtct
     1081 gaaggccgag ggtatgaagg atctttcacg gaaggctacg cccggcgggt tcaatcgcga
     1141 cacttctttg tagagcagct ccgggtctat ccatggggcg ccgatgtact tgaagggatg
     1201 taccgttccc ctgcccagag agaggttcgt gccttcgaaa aggcaaaagc cggtgtagag
     1261 aagcgcatgc tcgaaggaag gtatgttcgg cgacggtgtg ttccagaggt gtcccgtttc
     1321 atcgaagtac atattcctgt cgtagttttc catcggaacg acttccagat ccgctcccat
     1381 gttgaaggcg gcgttgtaat acctcgccag ttccccgaca gtgaggccgt atctaagcgc
     1441 gaggccgtaa cctcccacga atgtctcgaa gcccttcttg atggtcggtc cctcgacttt
     1501 gcaggaaagc gggttcggcc tgtctaggac gattactttg ataccccttc tggcacactc
     1561 ctccatcgag taggccagcg agtagatgta cgtgtaaaag cgcagtccca catcctgaat
     1621 gtcgtacacg aagaggtcga ggtctgagag catctcttcg gtgggcctgt aaatctcccc
     1681 gaagagtgaa taaaccggga taccgtacct tggatcgact gcgtttccca cccccactcc
     1741 gtcaggggcg gcgccggata taccgtgctc ggggccgaac agcttgacca gatcgaatcc
     1801 cgacgacacg atcaggtcga tgttccgttt caaccggccg ttgattcccg tcgagttcgt
     1861 cagaaggccc agcctctttc ctttgaagtc ctctctctct agaaagccgt ctattcccag
     1921 tttcactttc agttcagaca cctcacttta
//
LOCUS       DAOOLY010000367         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_74001_length_1950_cov_3.306069, whole genome shotgun sequence.
ACCESSION   DAOOLY010000367 DAOOLY010000000
VERSION     DAOOLY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74001_length_1950_cov_3.306069"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1950)
                     /locus_tag="PLD15_12240"
     CDS             complement(<1..>1950)
                     /locus_tag="PLD15_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB64730.1"
                     /translation="AWITPAAESLNTSDNEERARRRIEMAEKAVGTSDEALLDRMDTM
                     DTIAGIFYEKQTILSDYLKDYAIVFVRPDEAFAEYSRRERETIELLSDRPVRKFLYVR
                     YGGVSSEVFLKMADYTVVSDSAVTLMPYDSEQTETLEAVRREPKEREEEFIPRVPIVD
                     WTELEERDLVVHKEYGIGRYLGVRTIENVLGVREYLVLEYRDNNKIYVPVERVDRVHK
                     YVGNSDIVQLNSLKGNGWGRQKSKVKREVKALIGELSNLYGSREIVRGIPLAGDAEME
                     KSFRDSFPYVETDDQLRAVDEVFSDLQSGRPMDRLISGDAGYGKTEVAIRAAFRSVIS
                     GKQVALMVPTTVLARQHHENFSSRLTPFGIRVEILDRYRTENERSRLLRKLKKGEIDV
                     VVGTHSLLSREVQFADLGLVIVDEEQLFGVMQKEHFKKLRLQINVLSMSATPIPRTLY
                     MSISGLRDMSVISTPPAGRTTPEIYVGSVNDRLIRTAVLREVNRGGQTIYVHNRVNEL
                     PDLLLRLRELLPEVGIEMAHGQMNKTAFERTIRSFYTGSLDVLLCTTIIESGVDVPNA
                     NTLIVDDSHRYGLAQLYQLRGRVGRSTRRAFSYFLYDARRLTEPSRERLKALKEFSGA
                     GSGMKLAMRDLEIRGFGTLLGAEQHG"
BASE COUNT          361 a          592 c          496 g          501 t
ORIGIN      
        1 ccatgttgct cggcacccag aagcgttccg aaacccctta tctccaaatc tctcatcgcc
       61 agcttcatac cgctcccggc accggaaaat tccttgagcg ccttcagcct ctcacgcgag
      121 ggttctgtaa gtctcctggc gtcgtaaagg aagtaggaga aggccctcct ggtgcttctc
      181 cctacacgtc ccctgagctg gtaaagctgc gccagtccgt aacggtggga gtcgtcgact
      241 atcagcgtgt tggcgttggg cacgtcgacg ccgctctcga ttatggtcgt gcagagaaga
      301 acgtcgaggc ttccggtgta gaaagacctt atcgtccgtt cgaaggccgt cttgttcatc
      361 tggccgtgcg ccatttcgat gccgacctcg ggcagcagtt ccctcagtct caacaggaga
      421 tcgggcagtt cgttgaccct gttgtggacg tatatagtct ggccgcccct gttgacctcc
      481 ctcaggacgg ccgtcctgat cagcctatcg ttgaccgatc ccacgtatat ttccggcgtg
      541 gttctccctg ccggaggcgt ggaaataacc gacatatccc tcaatcccga aatagacatg
      601 tacaaagttc tgggaatggg cgtcgcgctc atcgagagaa cgttgatctg aagcctgagc
      661 tttttgaaat gttccttctg cataaccccg aaaagctgct cttcgtcgac tatcaccagt
      721 ccgagatcgg cgaactgaac ctccctggag agcagcgagt gcgttcccac cacaacgtct
      781 atctctccct ttttgagttt tctgagaagt ctgctcctct cgttttccgt tctgtaacgg
      841 tcgaggatct cgacccttat gccaaaaggg gtgaggcggg aggagaagtt ctcgtggtgc
      901 tgtcgcgcga gcacggtcgt ggggaccatc aaggccacct gcttgccgga gattacgctc
      961 ctgaaggccg cccttatggc cacctcggtc tttccgtaac ccgcgtcccc gcttatgagc
     1021 ctgtccatag gcctgccgct ctgaaggtcc gaaaagactt catcgacggc cctgagctga
     1081 tcgtcggttt caacgtaggg gaaactgtcc ctgaagctct tctccatctc ggcatccccc
     1141 gcgagcggta ttcccctgac gatctccctg gaaccgtaga gattgctcaa ctcgccgatc
     1201 agagccttta cctcgcgttt cactttggac ttctgcctgc cccagccgtt acccttgagt
     1261 gagttgagct gcactatgtc ggaatttccg acgtatttgt ggactctatc tactctttcg
     1321 acgggaacgt agatcttgtt gttgtctctg tattcgagaa ccaggtattc tctcaccccg
     1381 agcacgttct cgatggttct cacgccgaga tagcgtccga tgccgtactc cttgtgaacg
     1441 acgagatctc tctcctccag ttccgtccag tcgacgatcg gcactctggg aatgaactct
     1501 tcctctctct ccttcggctc tctcctgacc gcttccaggg tctcggtctg ttcgctgtcg
     1561 tagggcatca gcgtgactgc cgaatccgag actacggtgt agtcggccat cttcaggaag
     1621 acctcggaag agacaccccc gtacctgacg tacagaaact tgcgtaccgg tctgtcggaa
     1681 aggagctcga tcgtctctct ctcccttctc gaatactcgg cgaaggcttc gtctggcctg
     1741 acgaaaacta tggcgtaatc cttcaggtaa tcggacagaa tcgtctgctt ttcgtagaat
     1801 atgcccgcga tggtatccat ggtgtccatt ctatccagga gcgcttcatc acttgtgcca
     1861 acggcctttt cggccatttc gattctcctt ctggctctct cttcgttatc gctggtgttg
     1921 aggctctcgg cggcaggcgt gatccacgcc
//
LOCUS       DAOOLY010000368         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_74175_length_1947_cov_4.119979, whole genome shotgun sequence.
ACCESSION   DAOOLY010000368 DAOOLY010000000
VERSION     DAOOLY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74175_length_1947_cov_4.119979"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLD15_12245"
     CDS             <1..498
                     /locus_tag="PLD15_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HPB64731.1"
                     /translation="IWWGAAAPHEVVLVPLYCGITDVPDAFDTGSLEKFDRDVASWAF
                     NFMGNWAELKFSYMYPEIKEFQKKTEGKLFAMQSAIEAAAAQLYAVDPELCKEFLTDY
                     VADVSDRVMAETWEFNEYLITKYRDGYINIPNVGASAGYPDAWLKEVGYADGHIFGDD
                     GYKAK"
     gene            616..1248
                     /locus_tag="PLD15_12250"
     CDS             616..1248
                     /locus_tag="PLD15_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPB64732.1"
                     /translation="MKKVILVLVAISVVTLALATTDVKLGLPLAITSAGQSAEVETVN
                     YVADEVGLKYDYCDILSKDEFAAGVGLADAKSGIGKHVTTLSPDPKGTKFQTLILVVG
                     ADLKGMGASGLSVDSEVARLKNLIEYAKANGIKIVAVAIGGEIRRGLPGSPNEVMIDT
                     VIPSADIFVFTSDSNKDGRFSNEAKARNVPAVEIESAFDLLDTFTTIFGL"
     gene            1318..>1947
                     /locus_tag="PLD15_12255"
     CDS             1318..>1947
                     /locus_tag="PLD15_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPB64733.1"
                     /translation="MSADAIYMQTIIAGIVMVGAYVVGKLFKFSTEMCMFLAVVGGGI
                     AGGAGFDLFRHLAEGSVTYLDIGLIFVFATIFMNIIKESGGTNYIVRKIVGAFYNKKI
                     LMLILLMCVILVPGALTGAGSISVLVVGGTVAAALGAMGLSKVTISAIIFIVAGLSAA
                     APPINIWAMMTCANTAIPYVGFTMPLLIPIIILGLFTVLFLGRKAKVIDR"
BASE COUNT          484 a          460 c          517 g          486 t
ORIGIN      
        1 atatggtggg gcgcagcggc acctcatgaa gttgttctcg ttcctttgta ctgtggtatc
       61 accgatgttc cagatgcgtt cgacacggga agtcttgaaa agttcgatcg cgatgttgcg
      121 tcctgggcct tcaacttcat gggcaattgg gccgagttga agttcagcta catgtacccg
      181 gaaatcaagg aattccagaa gaagaccgaa ggcaagctct tcgcaatgca gtcagcgata
      241 gaggcggccg cagcacagct ttacgcggta gatcctgaac tctgtaagga attcctgaca
      301 gactacgtcg ccgatgtctc ggacagagta atggcggaaa catgggaatt caacgaatac
      361 ctgataacca agtaccgcga cggctacatc aacatcccca acgtcggtgc gtctgcaggc
      421 tatccagatg catggctcaa ggaagtcgga tacgccgatg gtcatatctt cggagatgac
      481 ggttacaaag cgaagtaatc actattgcct gtcccctgat ggggacaggc ttctatattt
      541 tttttatcag tggctttgaa aatgattttg ttttgagata aagcattcca taaaaactca
      601 aaaggagggt ttcagatgaa aaaagttata ctggttcttg ttgccatctc tgttgtaaca
      661 ctggcgctgg caaccacgga tgtcaagctg gggcttccgc tggcaataac ctctgccggc
      721 cagagcgccg aagttgaaac cgtaaactac gtcgccgatg aagtcggctt gaagtacgat
      781 tactgcgaca ttctctccaa agatgaattc gcggccggcg ttggactggc agacgccaag
      841 tctggaattg gcaagcacgt cacaactctt tctcccgatc caaagggcac gaagttccag
      901 acactcattc ttgtagtggg cgcggatctt aagggcatgg gcgcatccgg tctttcggtg
      961 gatagcgaag tcgcaagact gaagaacctt atcgagtacg ccaaagccaa cggtataaag
     1021 atcgtcgccg tagccatcgg tggagagatc agacgcggac ttcccggaag cccgaacgaa
     1081 gttatgatcg acacggttat cccttctgcg gatatatttg tgttcacgag cgactcaaac
     1141 aaagacggac gcttctcgaa cgaagctaaa gcaagaaacg ttccggctgt agaaatagag
     1201 tcggctttcg atcttctgga tacgttcacc acgatcttcg gcctttgatt ttcccattga
     1261 ccggcgttat cacagggggt tgtgagggcg ccggtctaat tgaagggggt agtaaaattg
     1321 tctgctgacg caatttacat gcaaacgatc atagcaggta tcgtaatggt cggggcgtac
     1381 gtggtcggga agttgttcaa gttctcgacg gaaatgtgta tgttcctggc agtggttggc
     1441 ggaggtatag caggaggcgc cggatttgac ctcttcaggc accttgcgga aggatccgtc
     1501 acctatctcg atatcggttt gatctttgtt tttgcaacga tcttcatgaa tatcatcaaa
     1561 gaatccggtg gaacgaacta catagtgagg aaaatcgtag gagctttcta taataaaaag
     1621 atcctcatgt tgattctcct gatgtgtgta atcctcgttc cgggagccct gaccggagcc
     1681 ggaagcatct ccgttcttgt cgtgggcgga accgtcgcgg ccgcgttagg tgcgatgggg
     1741 ctttcaaaag tcacgatatc ggcgataatc ttcatagtcg ccggtctcag tgctgccgct
     1801 ccaccaatca acatatgggc catgatgacc tgtgcgaaca cagccatacc ttatgttggc
     1861 tttacgatgc cgcttctaat acccatcatc attctcggtc tcttcaccgt gttgttcctt
     1921 ggaagaaagg ccaaagtcat tgacagg
//
LOCUS       DAOOLY010000369         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_74228_length_1946_cov_4.884717, whole genome shotgun sequence.
ACCESSION   DAOOLY010000369 DAOOLY010000000
VERSION     DAOOLY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74228_length_1946_cov_4.884717"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLD15_12260"
     CDS             <1..847
                     /locus_tag="PLD15_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPB64734.1"
                     /translation="GLTDNLVVVRDGEELSLGEKRLVFYMTPMVHWPETMMTYEKTTG
                     VIFSGDAFGGFGALNGGIFDDEVDVDYYKDEVLRYFSNIVGRYSAMVQKAFDKLKGVD
                     IKIICATHGPVWRSDPAHIINLYDRWSKQQTEEGVVLVYGSMYGNTQKMMEAVATGLV
                     KGGVEKIRVHNSSTSHLSFIIRDIWRFKGLVLGSCTYNMELFPPMKQLVSAIENRMMV
                     NRNIGVFGSYTWSGGALKELQEFAGKSKCEQVGPVIEAKSSPTAEDIDRCVELGMNMA
                     KAVKS"
     gene            1154..1849
                     /gene="msrA"
                     /locus_tag="PLD15_12265"
     CDS             1154..1849
                     /gene="msrA"
                     /locus_tag="PLD15_12265"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPB64735.1"
                     /translation="MGKRSLVISLIIITGVFSMAAIHKPEIDGEIPETLEYATFGLGC
                     FWGSEAVFGGVRGVYKTSVGYAGGTRMDPTYYSLGDHTEVVQIQFNPLEITYRELMEI
                     FWNSHNPYSKSYKRQYMSLILYHNDQQKRAAEEFIYSRKAETGLEAATELSPYDKFYR
                     AEDYHLKFYLQRDKQIMADLLRYYGDIFSLDGSTAAARLNALLAGKGDKTLFEEEINS
                     YGLASATVSSLGM"
BASE COUNT          556 a          441 c          525 g          424 t
ORIGIN      
        1 cggtttgacg gacaatctgg tcgtagtcag ggacggagag gaactctctc tgggcgaaaa
       61 gagactcgta ttctacatga cccccatggt tcactggccc gagacgatga tgacctacga
      121 aaaaaccacc ggcgtgatct tctcgggaga cgcttttggc ggcttcggcg ccctgaacgg
      181 cggcatcttc gacgacgagg tcgatgtgga ttactacaag gatgaggtcc tgagatactt
      241 ctccaatata gtcggaaggt acagcgccat ggtccagaag gccttcgaca aactcaaagg
      301 agtcgatata aaaataatct gcgccacgca tgggccagtg tggagaagcg atccggcgca
      361 tatcataaac ctgtacgatc gatggagcaa acagcagacc gaagaaggcg tggtgctggt
      421 ttatggttcc atgtacggca acactcagaa gatgatggag gccgtagcca ccggactcgt
      481 gaagggagga gtcgaaaaga tacgcgttca caacagttcg acttcgcacc tctcgttcat
      541 cataagagac atctggagat tcaagggact ggttcttgga agctgcactt acaacatgga
      601 gctcttccca ccgatgaagc aacttgtgag cgcaatcgaa aaccggatga tggtaaaccg
      661 caatatcggt gtcttcggtt cctacacatg gagcggcggc gctctcaaag aactccagga
      721 attcgcgggc aaatccaaat gcgagcaggt gggaccggtg atagaggcca aaagttcacc
      781 gacggccgaa gatatagatc gttgcgtgga actgggaatg aacatggcaa aggccgtcaa
      841 aagctgaaat ctgttcctgg tgaaaaaaag tacatagaga cttaaaaaag ggtcacaaaa
      901 ggttaattga tcagggtcaa aaatggacta gaatctagat gaaaagatca gatggaaggt
      961 tctatagatt catgacccca attgaatcgt tcacataaaa catgctaaac cccacccctg
     1021 ttcggaaatt ttcccaattg aaaatgggtc ctcaaagaga ggacccattt cacttatatg
     1081 cagtgaactc tgtgattgga taaaacgtcg aaatataacc gataattata gtttggtttt
     1141 tggaggtaaa atgatgggca agagatcgct ggtgatttcg ttaatcatca taacgggagt
     1201 gttttctatg gcagctattc ataaacctga gatcgacggc gagattcccg aaacgctaga
     1261 atacgcgact ttcggacttg gttgtttctg gggatctgaa gcggtatttg gcggagttcg
     1321 cggtgtatat aagacgagcg ttgggtatgc cggtggaacc aggatggacc ccacctatta
     1381 ctctctcggc gaccacaccg aagtggttca gatacagttc aatcctctcg agataactta
     1441 tcgcgagctg atggagatct tctggaactc acacaatcct tacagcaaaa gctacaagag
     1501 gcagtacatg tcgcttatcc tgtaccacaa cgaccaacag aagagggcag ccgaagagtt
     1561 catttactcc cgcaaggccg aaacgggatt ggaggccgcg acggagctct ctccgtacga
     1621 taagttctat cgtgccgaag attatcacct gaagttctat cttcagcgag acaaacagat
     1681 aatggccgat ctgttgaggt actacggaga tatattctcg ctcgacggtt cgaccgctgc
     1741 ggcgaggctc aacgcgctgc ttgccggtaa aggcgacaag acactcttcg aggaagaaat
     1801 aaacagttac ggcctggcca gcgcaacggt ttcatcactc gggatgtaag aagaacgaga
     1861 agagctgtcc ttcgtcagtc gtcccagagc gtggacccgt ccttggggaa cctgaggcta
     1921 gaggtaagag gcaagaggct ggaagg
//
LOCUS       DAOOLY010000370         1931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_75111_length_1931_cov_3.175906, whole genome shotgun sequence.
ACCESSION   DAOOLY010000370 DAOOLY010000000
VERSION     DAOOLY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75111_length_1931_cov_3.175906"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLD15_12270"
     CDS             complement(<1..734)
                     /locus_tag="PLD15_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPB64736.1"
                     /translation="MAFDVAVIGAIGIDTNVYLYTDEVDFSVEANFSQNLDYIGLAGG
                     YSCRGFKRLGYNVAFVGYVGDDYSGRHIRESLEKDGIDTYFGIDPEGTKRSVNIMYGD
                     GRRKNFYDGKGSMQLRPDLQAIGKYLAGTGLVHINIVNWTRYLLPLLKEENTVVSVDI
                     QDVVSPDDPYRQDYIKAADVLFFSAVNFPDPAPLIERFMEIRSDRIVICGMGKSGCAL
                     GTEKGISYYRAVELEEPVIDTNGAGD"
     gene            complement(752..1327)
                     /locus_tag="PLD15_12275"
     CDS             complement(752..1327)
                     /locus_tag="PLD15_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB64737.1"
                     /translation="MKKYDIFLAGVGGQGIGLLSETLARAVDYSGQRVIGADTHGLAQ
                     RGGMVSSHLRVGTGAHSVLITRHSADLVVALERHEALRGMLDYLKDGGTLVYYDAVWQ
                     PLDVRLRRTAQVRNEDIEAEAARKKAKVLRVFKEDLADSRMQNVIIMAELSKAKLIPG
                     VEKEHYTAALSDLLSGRVLEVNLKAFEEVAG"
     gene            complement(1337..>1931)
                     /locus_tag="PLD15_12280"
     CDS             complement(1337..>1931)
                     /locus_tag="PLD15_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB64738.1"
                     /translation="STGTAAGLSMFNDSRRVVAFLGDSTFFHAGLPGIVNAVFNKHNL
                     TLIIMENGTTAMTGHQDHPAVGRNFNDVSQKIPVRQVLEGLGVSNIREIDTYNQAGLK
                     EYVEAALEEEGFKVIIARHPCMLKFTREMRKKGKKLPRPVRVGEKCNLKYVCVGQFAC
                     PSYQIADGGRVWVQEDLCIGDRSCVQTCPSQAIEPVE"
BASE COUNT          364 a          581 c          497 g          489 t
ORIGIN      
        1 ctgtctccgg caccgttggt gtctatcacg ggctcttcaa gctctacggc ccggtaatag
       61 cttataccct tttccgttcc gagcgcgcaa ccactcttcc ccatcccgca gatcactatc
      121 ctgtcgctcc tgatctccat gaatctctcg atcagaggcg ccggatcggg gaaattcacg
      181 gccgaaaaga agagaacgtc ggcggccttt atatagtcct gtctgtacgg atcgtcgggt
      241 gagacgacgt cctgtatgtc gacagagacg accgtgttct cttcctttaa cagcggcaac
      301 aagtacctcg tccagttgac tatgtttata tgtaccagcc ccgtaccggc caggtacttt
      361 ccgatggcct gcaggtctgg cctgagttgc atcgacccct tgccgtcgta gaagtttttt
      421 cggcggccgt ccccgtacat tatattgacg cttcgcttgg taccctcagg atcgattcca
      481 aagtaagtgt ctatgccgtc cttctcgaga ctttcgcgta tgtgtcttcc cgagtaatcg
      541 tcgcccacat aaccgacgaa ggccacgttg tagccgagcc ttttgaaacc cctgcaggag
      601 tagccgcccg ccaggcctat gtaatcgagg ttctggctga aattggcctc caccgagaaa
      661 tcgacttcgt cggtatagag atagacgttg gtgtctatgc ctatggctcc tatcactgct
      721 acgtcgaagg ccatcggtca acctccgttg ctcatccggc gacttcttcg aaggccttga
      781 ggttgacttc cagcacccgc ccactcagga ggtcggacaa agccgctgtg tagtgttcct
      841 tctccacgcc cggtatgagt ttcgccttcg agagctcggc cattatgatc acgttctgca
      901 ttctcgaatc tgcaaggtcc tctttgaaga ctctcaggac cttcgccttt tttctggcgg
      961 cctccgcctc tatatcttcg ttcctgacct gggccgttct gcgcagtcta acatccaggg
     1021 gctgccatac ggcatcgtaa tagaccagcg ttccgccgtc tttgaggtaa tcgagcatgc
     1081 ctctgagggc ttcgtgcctc tccagagcca cgacgagatc ggcgctgtgt ctggttataa
     1141 gtacggaatg ggcgcctgtg cctactctaa ggtgagaaga gaccatgcct cccctctggg
     1201 cgagaccgtg cgtatcggcg cctatcactc tctgtcctga ataatcgacg gcccgggcga
     1261 gcgtctcgct gagaagccct atgccctgac ctccgacacc tgccagaaat atatcgtatt
     1321 ttttcatctc ttcacctcac tcgacaggct ctatggcctg cgatggacac gtctggacac
     1381 aggaacggtc gcctatgcag agatcttcct gcacccacac tctgccgccg tcggctatct
     1441 ggtaactggg acaggcgaac tggccgacac agacgtactt gaggttgcac ttctctccga
     1501 ccctgacagg cctgggcagt ttctttccct ttttacgcat ctccctggtg aacttcaaca
     1561 tgcagggatg cctggcgatg attaccttga agccctcttc ttcgagcgcc gcttcgacgt
     1621 actccttcaa cccggcctgg ttgtaagtgt ctatctcgcg gatgttgctt acgccgaggc
     1681 cttccagcac ctgcctgacc ggtatctttt gcgatacgtc gttgaagttg cggcccacgg
     1741 ccggatggtc ctggtgaccg gtcatcgccg tggttccgtt ttccatgatt ataagggtga
     1801 ggttgtgttt gttgaaaacg gcgttcacta ttccgggcag gcccgcgtgg aagaaggtgg
     1861 aatcgcccag gaaggcgacc acccttcggg aatcgttgaa catcgaaaga ccggcagccg
     1921 taccggtgga a
//
LOCUS       DAOOLY010000371         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_75697_length_1921_cov_3.734191, whole genome shotgun sequence.
ACCESSION   DAOOLY010000371 DAOOLY010000000
VERSION     DAOOLY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75697_length_1921_cov_3.734191"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>1921
                     /locus_tag="PLD15_12285"
     CDS             219..>1921
                     /locus_tag="PLD15_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64739.1"
                     /translation="MKKILLVTLALLVSLFAFASDPNTLVNLTIGEPETLDGMHSYDT
                     ASGEVIENLYDNLIQYNGQSVTEFLPMLSTEVPSVDNGLVVEEDGALKYIFPIREGVK
                     FHSGNILTPADVEYSFERYILGDPAGKAHWMIIEALTSSAFYTVEDWFADYAGMAYSD
                     AVNADRNPISDEAKAKLVSFYEEVIDPRIEVVDNTVVFTLDGPFGAFLNIVCHFGSWG
                     AIVDSKLAMERGDWDGKADGWWKWHDLEADEYAFHDFGGGSGPYKLVEWDRAQQKVVL
                     ERFDDYWAGPAAIKNVVIWGVDEFSTRKSMFEAGDADIAYFDPVYLDQALALEAQGKA
                     EVTTGYPVSSITSLHFNWLIAEGSEYLGSGKLDGNGIPREFFSDIHVRRAFAFCYDGI
                     TFIDEVINGLGKLVPTDLPEGYLGFDETLPLPEFNLARAAAEFKQAWGGEVWNKGFKL
                     QILYNTGNNSRQTVAEMLSYFVSSINPKFKVEPLGVQWPTYLSAQNNGFLPMFIIGWV
                     ADYPDPHNFISTYYASFGTYAGRQGQLFREWAIENVDDEIVAAIKATDSAERIKLYKS
                     V"
BASE COUNT          495 a          490 c          489 g          447 t
ORIGIN      
        1 acctacggcc tcttcctcgt caaggaagcg ctcttccact gagctacgca cccggacagg
       61 agagattcta ccattgtaac cgtacaattg tcaatatgta tatgaggctt gaaaacccca
      121 aatacaacac aatggttaac gcagttttga atttcgaagc tcgatagatt ataattgttc
      181 tgcacacact catctctatt tgaaaggggg caacactagt gaagaaaatt ctactggtaa
      241 cacttgctct gttagtatca ctctttgcgt ttgcaagtga cccaaacacg cttgtgaatt
      301 tgaccatcgg tgaacctgag acactcgatg gaatgcactc ctacgatact gccagcgggg
      361 aggtcataga aaacctctac gacaacctga ttcagtacaa cgggcagtcg gtcacagagt
      421 tcctgcccat gctctccaca gaagttccga gcgtcgataa cggtcttgtg gttgaggaag
      481 atggcgctct gaagtacata tttcccataa gggaaggcgt caagttccac agcggaaaca
      541 tacttacccc ggcagatgta gagtactcct ttgagagata catactcggc gatcccgctg
      601 gcaaggctca ctggatgata atcgaagctc ttaccagctc ggcgttctac acagtcgaag
      661 actggtttgc cgattatgcg ggaatggcat attccgatgc ggtaaacgca gacaggaatc
      721 caatctccga cgaggccaag gccaagctcg tctccttcta cgaagaggtc attgatccga
      781 gaatcgaagt agttgacaac actgtcgtct tcactctcga tggtcctttc ggcgccttcc
      841 tgaacatagt gtgtcatttc ggctcctggg gagcgatagt cgattccaag ctcgccatgg
      901 aacgtggtga ctgggatgga aaggccgacg gctggtggaa gtggcacgat ctggaagccg
      961 atgaatatgc attccacgac ttcggaggcg gttccggtcc gtacaagctg gtcgagtggg
     1021 acagggcaca gcagaaggtt gttctcgaga gattcgacga ttactgggca ggtccggcgg
     1081 ccataaagaa cgtcgtgatc tggggtgtcg atgaattctc taccagaaag tccatgttcg
     1141 aagccggcga tgccgatatc gcttacttcg atccggtcta tctcgatcag gctctggcac
     1201 tggaagcaca gggtaaggcc gaagttacga ccggttatcc agtttcctcg ataacctctc
     1261 tacacttcaa ctggctgatc gcagaaggca gtgagtatct cggaagcgga aagcttgacg
     1321 gaaacggtat cccgagagaa ttcttctccg atatccacgt aagaagggct ttcgctttct
     1381 gctacgatgg catcacattc atcgatgaag ttataaatgg tctcggtaag ctcgttccga
     1441 cagatcttcc cgaaggttac ctcggcttcg acgaaaccct gccgttgccc gaattcaatc
     1501 tggccagggc cgctgccgaa ttcaagcagg cctggggcgg agaagtctgg aacaagggtt
     1561 tcaaactcca gatactctac aacaccggta acaattccag acagactgtc gccgaaatgc
     1621 tctcctactt cgtgagttct atcaacccga agttcaaggt tgagccgctc ggtgttcagt
     1681 ggccgacgta cctctcggca cagaacaacg gattccttcc gatgttcata ataggctggg
     1741 tcgcagacta tccagatcca cacaacttca tatccacgta ctacgcgagc tttggtacct
     1801 acgcaggaag acagggacag ctgttcagag agtgggcaat agagaacgtc gacgatgaaa
     1861 tcgtcgccgc gatcaaggca accgactccg cggagagaat taagctttat aaatccgttc
     1921 a
//
LOCUS       DAOOLY010000372         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_75700_length_1921_cov_3.527867, whole genome shotgun sequence.
ACCESSION   DAOOLY010000372 DAOOLY010000000
VERSION     DAOOLY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75700_length_1921_cov_3.527867"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLD15_12290"
     CDS             complement(<1..133)
                     /locus_tag="PLD15_12290"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPB64740.1"
                     /translation="MNLIAAGKKLIELLDIEFPIIQGGMAWIADHSLASAVSNAGGLG
                     "
     gene            290..676
                     /gene="fabZ"
                     /locus_tag="PLD15_12295"
     CDS             290..676
                     /gene="fabZ"
                     /locus_tag="PLD15_12295"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPB64741.1"
                     /translation="MSRIPHREPFLMVDGVIEMGTSHIKAFKDINEKLEVFKGHFPNY
                     PIYPGVLIIEGLAQTAGVLLLEPGSTPLFAGIERARFRGQVRPPCVLEYEVKVIQTKM
                     GVVKVEAMASVEELPVAEAVLLLARA"
     gene            complement(783..>1921)
                     /locus_tag="PLD15_12300"
     CDS             complement(783..>1921)
                     /locus_tag="PLD15_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB64742.1"
                     /translation="EGYIFTNEHVVAGANKITVSLLDGSVYPAKYIGGDADLDIAVIK
                     IDPDGRDLPIAELGDSDTLRVGEWAIAIGNPLGLKHTVTVGVISAVGRQIPKPDGNSV
                     YSNLIQTDAAINPGNSGGPLLNIYGQVIGINTAIVDPDVGTTLGFAIPVNVALRFVDS
                     IIATGSIQRAYLGVYLDNVTESTARSLGLKIDKGALVMDVVKGSAADKAGIKPQDVIV
                     KFQNLEINNSSELRAAILNYPAGSEVSVVVNRFGETIELKVILGTMQEQVQTKAPQEQ
                     KPAESSVLGITVNDITPYDRERLSLPEDLRGVIVSGVNTQGIVYRLGLSIDDVITRIS
                     VNGNQQAIQGVDDFKKVVDSLKKGDYIAFFIYRDGVRFVASFQY"
BASE COUNT          457 a          477 c          454 g          533 t
ORIGIN      
        1 cgccaagccc gccggcattc gaaactgcgc tcgccaatga gtgatctgcg atccaggcca
       61 ttcctccctg aattattgga aattctatat ctagaagttc aatgagtttt tttccggccg
      121 ctatcaaatt caccgctcct ttggcatctt tgtgcttcta attcgaagca aagatatacc
      181 atagaaaaat agggagttaa cgtacgattt ccggtcggtt tttccaactg gtcgtctgga
      241 aagcgtacag aaaggagacg aaacaatgga aatggacaga gagtttgtaa tgagtaggat
      301 cccacacaga gaaccctttc tcatggtgga tggggttatc gaaatgggaa cctctcacat
      361 taaagcattc aaagatatca atgaaaaact cgaagttttc aagggtcact tccccaacta
      421 ccccatatat ccgggcgtgc ttataatcga aggactcgct cagacggccg gggtgcttct
      481 tctggaacca ggctctacac cgctatttgc aggtatcgag agagcccgct tcaggggtca
      541 ggttagaccg ccttgcgtgc ttgaatatga agtgaaagtt atacagacga agatgggtgt
      601 cgttaaagtt gaggcgatgg cgagcgtaga ggaactgcca gtagccgagg ctgtcctttt
      661 gcttgcccgc gcgtagtttt gaaagaaaaa tcccctgtgc tataataatt catatgattc
      721 agcacagggg ttaacggggg taagccgcca ctcgaaagag tggcggtgtt tccttttgat
      781 ccttagtact ggaatgaggc tacgaatctc actccgtcgc gatagataaa gaaggctatg
      841 taatcgccct tcttcaagct atcgacaacc ttcttgaaat catcgacacc ttgtatggcc
      901 tgttgatttc cgttcacact tattctggtt atcacatcgt cgatacttag tcccaatcta
      961 tagacgatac cctgcgtatt cacaccgctg actatgacgc ctctgagatc ttcgggaaga
     1021 gaaagcctct ctctatcgta tggagttata tcgttcactg taataccaag aacgctgctc
     1081 tcggctggct tctgttcctg tggggctttc gtctgcacct gttcctgcat agttcccagt
     1141 atcactttaa gctctatggt ttcaccgaat ctgttcacca cgaccgacac ttccgaaccg
     1201 gctggataat tgaggatggc agctctcagt tccgatgaat tgttgatttc gagattctgg
     1261 aatttcacta tgacatcctg cggttttatt ccggctttgt cggcggccga acccttcacg
     1321 acgtccatta ccagagctcc cttgtcgatt tttagaccca gggatcgtgc tgtcgattca
     1381 gtgacgttat cgagatagac tcccagataa gccctctgga ttgagcctgt cgcgatgatc
     1441 gaatctacga accttaaggc cacgttcacg ggtatcgcaa agccgagagt tgtgccgaca
     1501 tcaggatcga ctatcgctgt gttgatgccg ataacctggc catatatgtt taaaaggggt
     1561 ccaccgctgt tgccaggatt tatcgccgcg tcagtctgta tcagattgga atatacgcta
     1621 tttccatcgg gtttgggtat ctgtctgccg accgcgctta ttactcctac cgtaacggtg
     1681 tgcttcaatc cgaggggatt tcctatggct atcgcccatt ctccaactcg cagggtgtcg
     1741 gaatctccca gttcagctat gggaagatct cttccatcgg ggtctatctt tatgaccgct
     1801 atatcgagat cggcatcgcc accgatgtat ttggccgggt aaacgctccc gtcaagaagc
     1861 gagaccgtta tcttattggc tcctgcaacc acatgttcgt ttgtaaagat gtacccctct
     1921 t
//
LOCUS       DAOOLY010000373         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_77226_length_1895_cov_5.167935, whole genome shotgun sequence.
ACCESSION   DAOOLY010000373 DAOOLY010000000
VERSION     DAOOLY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77226_length_1895_cov_5.167935"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1895)
                     /locus_tag="PLD15_12305"
     CDS             complement(<1..>1895)
                     /locus_tag="PLD15_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretory pathway, component HofQ"
                     /protein_id="HPB64743.1"
                     /translation="KVGESVRVSTVLSVVPGWDQQKTFEYFHDFLGEDEYSRVSLTPS
                     AGGYIVVGPEETLKKLETEVSRLTQLEHPFFTIENSIPALDKFEQLLTTLGIKVNIIV
                     LEDKYLIVGTRENVISTSELLQQIIETVYSDDAVQEEPPVELGFTLIEAMAEDIPVLA
                     SVVTRLGIPVDLVGISTGAIVIGQADRLEEASGVIESILKHRESLYSKPELDYRFVDI
                     QTQLIDQLLPVMQKLEIQVALLETPTGVIAIGTPQELSRATGLVDSVMSREVPEIPQK
                     EKYESYLLLPLTQGLSVDALMQVLETFEYEISLLGISDKLIAIGREEDLVKFKSLYKS
                     LQAQEIDRESRVSIEIKSIPGWTAEDFGQYTRFFLGADDFARISILESLAGYVVLTPL
                     DLKEPIESEVERIRGFEDPEFVVKEKLPPVEDLTGLLARLGIIVDLVPMGSSAIIIGS
                     SDAVQRAGEIVDRLMGSLVVETVVEEPLEYRILDIPEEDTASLQNILDRLGVDVSLLS
                     SSSGVIAIGTGSTIEKASGIAGDILSKRISAQVEEPVYTFTEIPLADIQSYQAILNRI
                     GAGVELLPSPSGVLIVGTGERVEKALKTVNAVLAREVEMSSAGEQPRRVSTVLSVVPG
                     WSDEKYS"
BASE COUNT          421 a          532 c          448 g          494 t
ORIGIN      
        1 cggaatactt ctcgtcgctc cagcccggga cgaccgacag aaccgtcgag actctccttg
       61 gctgttcgcc ggccgaactc atctctacct ctcttgcgag aaccgcgttc acagttttca
      121 gtgccttttc gaccctttct ccggttccta cgataaggac tcccgaaggt gatggcagca
      181 gctcaacacc ggctccaatc ctgttcaata tggcctggta gctttgaata tcggccagtg
      241 gtatttctgt aaatgtataa accggctcct cgacctgagc gctgatcctc ttgctcagta
      301 tatccccggc tataccggaa gccttttcta tagtagagcc agttcctatg gcgatcacac
      361 ctgaagacga agagaggaga gatacatcta cccccagcct atcaagtata ttctgcagac
      421 ttgccgtgtc ttcttcggga atatcgagaa tcctgtactc cagaggttct tccaccaccg
      481 tctcaacaac caacgatccc atcagtctgt ccactatctc tccggccctc tgaactgcat
      541 ctgaggaacc tattattatc gccgagctgc ccatgggaac gagatcgact atgataccca
      601 gcctggccag gagccccgtc aagtcctcca cgggcggcag tttctctttc acgacaaatt
      661 cgggatcttc aaatcccctg atcctttcta cttcgctctc tatcggctct ttgagatcta
      721 aaggcgtgag aacgacatat ccggcgagtg attccagtat agatattctg gcgaaatcgt
      781 cggcccccag gaagaaacgc gtgtactgtc cgaaatcctc ggcggtccac ccgggaatac
      841 tttttatctc tatagatact ctactttcac ggtctatctc ctgcgcctga aggctcttgt
      901 aaagagattt gaacttcact agatcttcct cgcgcccgat agctatgagt ttgtcggaga
      961 tgccgagcaa ggaaatttcg tattcgaatg tctccagtac ctgcattaac gcatcgacac
     1021 tgagtccctg ggttaacggc agtagaaggt aactctcgta tttctccttc tgtgggattt
     1081 cgggaacttc tctcgacatc accgaatcca caagaccggt ggccctcgaa agctcctgtg
     1141 gagttccgat ggctatgacg cccgtcgggg tttcgagaag cgcgacttga atttcgagtt
     1201 tctgcatgac aggcaacaac tggtctatta gctgcgtctg gatatcgacg aatctgtagt
     1261 cgagttcggg ttttgagtag aggctctcac ggtgtttcag gatagattca atgacacccg
     1321 atgcctcttc gagtcgatcg gcctggccga taactatcgc tcctgtggag atgcccacca
     1381 agtcaaccgg aatgccaagc ctggtaacca ccgacgccag aaccggtata tcttcggcca
     1441 ttgcctcgat aagagtgaag ccaagctcta caggaggttc ttcctggaca gcgtcgtcgg
     1501 aatagacagt ctcgattatc tgctgtagaa gttccgaagt gctgatcacg ttttctctcg
     1561 ttccaacgat caagtattta tcttccagaa ctattatgtt gacttttatg cctagcgtgg
     1621 ttagtagctg ctcgaacttg tcaagggcgg ggatgctatt ctctatggtg aagaagggat
     1681 gctctagctg ggttagtctc gaaacctctg tctccagctt tttcagcgtc tcctccggac
     1741 caaccacgat gtagcctccg gccgaaggcg tgagcgagac cctggagtac tcgtcctccc
     1801 cgaggaagtc gtggaaatat tcgaaggtct tctgttgatc ccagcccggg acgaccgaca
     1861 gtaccgtcga gactctgacg ctctctccaa ccttg
//
LOCUS       DAOOLY010000374         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_77800_length_1886_cov_2.607318, whole genome shotgun sequence.
ACCESSION   DAOOLY010000374 DAOOLY010000000
VERSION     DAOOLY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77800_length_1886_cov_2.607318"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLD15_12310"
     CDS             complement(<1..1563)
                     /locus_tag="PLD15_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64744.1"
                     /translation="MVYFRGSSVVPVIITVLFVVVAAVVAVFFLWPAPEPRILITNVS
                     PIPRNGERVLAEKFIELKWEAVYERPRQGLMAEVFVGEDRNSLVKLAQDVEGTLTSQS
                     EGIFSFVFRYPVEPHSQYWWKVRVYNQNGKSVESEVWTFVLMNQYPEKPELIPPSQNS
                     ANVALRDLVLKWKASDLDNDELTYDVYFGREPRLDERDLLLKGTKDTEIDISTLKKLD
                     YSTKYYWKVVAKDPYGGESVSKTDSFTTQIRAELPALSPVMPVNGMTGFNADIASLSW
                     KTSLPLDYYPDGLFFDVYIAEGQSRASLVGTTNKTEMVVPSIKGHTSYNWYIVVRDAS
                     GKEKKSDQWTFTTANRAPVIDIQYPDLTTSSTSVPVSWTVIDRDGDEITSEVFFGEVN
                     ETPVRIASGKMTSIFLSGLNIGKQYMLKVVSKDGRGAQTEKEILLTPGNSTPSVVLSR
                     PGTDGTVEPTMVTFEWSGSDGDGDKLTYQLHINSPGQRQVFENIDIERFTVRDLQEER
                     EYSWYVIASDEKG"
     gene            complement(1624..>1886)
                     /locus_tag="PLD15_12315"
     CDS             complement(1624..>1886)
                     /locus_tag="PLD15_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64745.1"
                     /translation="HNHRHVLRMVSNNGEEKFYANTGTWKPVVELIDDNPDNGFARRV
                     ELGYITIEMIDDEFLVQSKHIKKLSAVRLAERTSRNGTFFEV"
BASE COUNT          454 a          467 c          423 g          542 t
ORIGIN      
        1 tcccttttca tcgcttgcta taacgtacca actatactct ctttcctcct ggagatctct
       61 gacagtgaat ctttcaatat ctatgttttc gaacacctgc cgctgtcccg gcgaattgat
      121 gtgtaactgg tatgtgagtt tatcaccgtc gccatcgcta cccgaccatt cgaaggtcac
      181 catcgtaggc tcgaccgtac catcggtacc aggtctggaa agtactacgg aaggggtact
      241 atttccagga gttagaagta tctccttttc tgtctgggca ccccgtccgt cttttgaaac
      301 taccttgagc atgtactgtt ttccgatgtt tagacccgag agaaatatgg aggtcatctt
      361 tcccgacgcg attctcaccg gtgtctcatt aacttctccg aagaagactt cgctggtgat
      421 ttcatcgcca tctctgtcta tgacggtcca gctaacggga acactggtcg atgaggtggt
      481 aaggtctgga tactgtatat cgattaccgg tgccctgttg gcagtggtga aagtccactg
      541 atcgcttttc ttttcctttc cggaggcgtc tcttacaacg atgtaccaat tataggacgt
      601 gtgacctttt attgatggaa cgaccatctc cgtcttgtta gttgtgccga ccagcgatgc
      661 tcttgactgt ccctcggcaa tatatacatc gaagaagagt ccgtccgggt agtagtcgag
      721 agggagagaa gttttccagg agagcgaagc aatatcggcg ttaaaacccg tcatgccgtt
      781 taccggcatg acaggcgata gagccggaag ctctgcgcgt atctgggtcg tgaaagagtc
      841 ggtcttggat acggactcgc caccataagg atcctttgcg acaactttcc agtagtactt
      901 tgtcgagtaa tcgagtttct tgagggtgga aatatctatc tctgtgtctt tagttccttt
      961 tagaagaaga tctctctcgt ccagtcttgg ttctcgcccg aagtaaacat cgtaggtcag
     1021 ctcgtcgtta tctaaatcgg aggccttcca cttaaggaca agatccctca gagctacgtt
     1081 ggccgaattc tgactcggcg gaataagctc tggtttttct ggatattgat tcattagaac
     1141 gaaagtccat acttcgctct ctacggactt cccgttctgg ttgtaaacac gaaccttcca
     1201 ccagtactgg gagtgtggtt ctactggata gcgaaaaacg aaggaaaaga ttccttcgga
     1261 ctgtgaagtg agggttccct ccacatcttg ggcaagctta acaaggctat tacggtcttc
     1321 tcctacaaac acttctgcca tcaaaccctg tctaggtctc tcatagacgg cttcccattt
     1381 caattctata aacttctcag ccaggaccct ctctccgttt ctagggatgg gcgaaacgtt
     1441 cgtgatcaat attctcggtt ctggagctgg ccaaagaaaa aacacagcca cgacagcagc
     1501 tactacgacg aaaagaactg tgattatgac cggcacaaca gatgagcccc tgaaatacac
     1561 cactgaatcc ctcctcagat acccggaccg ttggactcat tggaaggtct ttccaataag
     1621 attttatact tcaaagaaag ttccatttct ggatgtcctt tcagcaaggc gaaccgcact
     1681 aagttttttt atatgtttgg attgcacaag aaactcgtca tcgatcattt ctatggtaat
     1741 atagcctaac tcgactcttc tggcaaaacc gttgtccggg ttgtcatcta ttaattcaac
     1801 gactggcttc cacgtcccgg tgttggcgta aaatttttcc tctccgttgt tagataccat
     1861 gcgaagcaca tgtcgatgat tatgac
//
LOCUS       DAOOLY010000375         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_78850_length_1869_cov_4.162624, whole genome shotgun sequence.
ACCESSION   DAOOLY010000375 DAOOLY010000000
VERSION     DAOOLY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78850_length_1869_cov_4.162624"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLD15_12320"
     CDS             complement(<1..813)
                     /locus_tag="PLD15_12320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB64746.1"
                     /translation="MDLSIIIVNYNVKEFIQNLLESIKSASKKISTEVIIVDNASDDG
                     SIELIRQKYPWVKLIVNSRNLGFSKANNLGLKESTGKYLLLLNPDTLVKEDTFEKMID
                     FFEATPDAGMAGCKLLNTDGTLQLACRRGFPGPWASFTKVTGLSTLFPKSPLFARYNL
                     TYLDENKTYAVDAISGAFMMFKREVYEKTGGLDETFFMYGEDLDLCYRVNKAGYKVYY
                     VHSTEIIHYKGESTKRSNLNEVKVFYEAMHIFVKKYYSGSWFLLLFLRSAIFF"
     gene            complement(826..1548)
                     /locus_tag="PLD15_12325"
     CDS             complement(826..1548)
                     /locus_tag="PLD15_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPB64747.1"
                     /translation="MDKKTLIIVPTYNERENIENLLPVLFGLGVEGLHVLVVDDNSPD
                     GTGEYVQQQAELNEKIHILRRAGKMGLGTAYCAGFKYAIENGYDYVFEMDADFSHDPN
                     LVPKFLEKIGPADLVIGSRYLTGVNVINWPMKRLLLSYFANWYTRFITGLPIKDATGG
                     YKCFKVEVLKSIDLDKIQSNGYAFQIEMNFKAWKKGFKLDEIPIIFFDRFQGTSKMSK
                     KIVYEAVFMVWKLRFKSLFGTL"
     gene            complement(1571..>1869)
                     /locus_tag="PLD15_12330"
     CDS             complement(1571..>1869)
                     /locus_tag="PLD15_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB64748.1"
                     /translation="QIWNVFKGEMSLVGPRPERPFFVEQLAKEIPYYKRRLRVRPGIT
                     GWAQVKHKYDENIEDVRTKLQFDLFYIENMSLKTDIKILFRTVFVVLFGKGHYD"
BASE COUNT          460 a          468 c          420 g          521 t
ORIGIN      
        1 gaaaaatatt gccgagcgga ggaacagaag cagaaaccat gagccggaat aatacttctt
       61 tacaaagatg tgcatcgcct cgtagaacac cttcacttca ttcaggttgc ttctttttgt
      121 gctctcccct ttataatgga tgatctcggt tgagtgaacg taatagactt tatatccagc
      181 cttgttcact ctgtaacaga ggtccaggtc ttcaccgtac atgaaaaagg tctcgtcgag
      241 tccgcccgtc ttctcataga cctccctctt gaacatcata aacgcgcccg aaatggcatc
      301 aactgcatac gtcttgttct catcaaggta ggtgagatta taacgggcga aaagagggct
      361 tttggggaaa agtgttgaga gcccggtcac cttcgtaaat gatgcccagg gccccgggaa
      421 cccgcgcctg caggcgagtt gaagtgtacc gtcggtgttc agaagtttac accccgccat
      481 tcccgcatcc ggtgttgcct cgaagaaatc gatcatcttt tcaaatgtgt cttctttcac
      541 aagagtgtca ggattcagga ggaggagata tttcccggtg gattctttga gcccaaggtt
      601 attggccttg ctgaaaccga gattccggga attgacaatc agctttaccc atggatactt
      661 ctgccggatg agttcaatac ttccatcgtc agaggcattg tcaactatga tcacttcggt
      721 cgatatcttc tttgaagcac tctttatcga ctcaagcagg ttctggatga actcctttac
      781 attatagttg acaattatta tcgacaggtc cacagtagat cgcccttaga gcgtgccgaa
      841 caaacttttg aaccggagct tccagaccat gaaaaccgcc tcatagacga tctttttcga
      901 cattttcgag gtaccctgaa aacggtcgaa gaagattatc ggtatctcat caagcttgaa
      961 gcccttcttc caggctttga agttcatctc gatctggaat gcgtagccgt tcgattggat
     1021 cttgtcgaga tcgatcgact taagcacctc aaccttgaag catttgtagc cacctgtggc
     1081 gtcctttatc ggaagccccg ttataaacct tgtgtaccag ttagcgaaat aactgagcaa
     1141 aagccgcttc atcggccagt tgatcacatt caccccggtg aggtaacggc ttccgatcac
     1201 gagatcggca ggaccgatct tctctaaaaa ctttggaacg aggtttggat catgcgaaaa
     1261 atcggcatcc atctcgaaga cgtaatcgta tccattttca atggcgtact tgaacccggc
     1321 gcagtatgct gtaccaaggc ccatcttgcc tgcccttctg aggatgtgta tcttttcgtt
     1381 cagctccgcc tgctgttgaa cgtattcacc ggtcccgtct ggtgaattgt cgtccaccac
     1441 aaggacgtgg agcccttcta ccccaaggcc aaaaagcacg gggagcaggt tctcgatatt
     1501 ctctctctca ttgtaggtgg gcactataat taatgtcttc ttatccatat gtaatccgtt
     1561 tttcggggcg ttaatcgtaa tgtcctttgc cgaacagcac tacaaatacc gttctgaaca
     1621 gaatcttaat gtcggtttta agcgacatgt tctcaatata gaagaggtcg aactgaagtt
     1681 ttgttcttac atcttcaatg ttttcatcat acttgtgctt cacctgggcc caaccggtta
     1741 tcccgggcct tacgcgaagt cttcttttgt aatagggtat ctccttggcc aactgctcaa
     1801 cgaagaaggg gcgctccggc ctggggccta cgagactcat ctcgccttta aaaacattcc
     1861 agatctggg
//
LOCUS       DAOOLY010000376         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_79521_length_1859_cov_2.893570, whole genome shotgun sequence.
ACCESSION   DAOOLY010000376 DAOOLY010000000
VERSION     DAOOLY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79521_length_1859_cov_2.893570"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>1859
                     /locus_tag="PLD15_12335"
     CDS             81..>1859
                     /locus_tag="PLD15_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB64749.1"
                     /translation="MAATKDCGCRTISEDVSLDFSWFAELGNLEKLLRLSAVFEKGPV
                     LITEWLYVALLKFAPVFEGYFGKTAAINCVERFGAPIDETMPLEVLGTIFEIHSDSLR
                     IGKFLPIEEYPLYPRSKSQEVNTIDGLIAIIVGKKASAFSLSEDLKALLVERNYNLYN
                     GPDPEVIIVNSATEKQLAEIDRLISLAEAEGRTIDLVNFTGYCPGPEARSLDLKSELK
                     REFGFDSFKDYAVYGSDGTYSISQEEMIRYLIDQNYRKKPSDLFFVASTGSGKSLIYQ
                     LAAKILKKTLKRLTVVITPLKALMEDQVEILKERFGQTEAAFINSDLSLDERAELIDR
                     LHKGEITILYVSPETFVGCSLSTIIGDRRIGLMVIDEAHLVTTWGKTFRVDYGYLGED
                     LKVLRERMDFPVMATTATAISGGELDTVDEISNLLYLRSPRIIMTSVRRNNISFNITR
                     FNLSEEKKESRTSQKISDTVDSTISLIESGKKSIVYCPFVGQADNIYTVVSSVAECRN
                     KVGRYTGPTGVEERRKTQRLFGKGIYRTVIATKAFGMGVDIPDIDEVYHHSVPSVMAD
                     YVQEIGRAGRDGRPSIASTHFNVKDLS"
BASE COUNT          530 a          403 c          480 g          446 t
ORIGIN      
        1 ggcatgttga tatccaggag ggtgatgtcc ggccggaggg agcgaaaacg gtcgtactca
       61 aaaacgccgg gccggaatcg ttggccgcta cgaaagattg tggttgcaga acgatatccg
      121 aagatgtttc gctcgatttt tcctggttcg ccgaactggg taatctcgag aaactcctcc
      181 ggctctcggc cgtttttgaa aaaggccccg tgctgattac agaatggctg tatgtggctc
      241 tgctgaagtt cgcgccggtt tttgagggct attttggaaa aaccgctgct atcaactgcg
      301 tggagagatt cggagcgcct atcgacgaaa caatgcccct ggaagtcctt ggaacgattt
      361 ttgaaatcca ttctgacagc ctgagaattg ggaaatttct tcctatcgaa gagtaccctc
      421 tctaccccag gtcaaaatca caagaggtaa acacgatcga cgggctgata gcaattatcg
      481 ttgggaaaaa ggccagcgct ttttcattaa gtgaagactt aaaagccctg ctggtcgagc
      541 gaaattacaa tctctataac ggtcccgacc cggaagtaat tattgtcaac agtgcaacgg
      601 aaaagcagtt ggccgagata gacaggctca tatctctagc ggaggctgaa ggcagaacga
      661 tcgatctggt gaatttcaca ggttactgcc cgggaccgga agcgagaagc ctggatctta
      721 agtcggaact gaaaagggaa ttcggattcg attcattcaa agattacgca gtttatggaa
      781 gcgacggaac ttattctata agtcaggaag agatgataag atatttgatc gaccagaact
      841 atcgtaaaaa accttcggat ttgttctttg tcgcctctac gggctcaggg aagtctctga
      901 tttaccagct cgcagcgaaa atattgaaga agacccttaa aaggctcacg gtagttatca
      961 ctcctttgaa ggcactgatg gaggatcagg tcgagattct caaggagcgc ttcgggcaga
     1021 ccgaggccgc tttcataaac tccgatctga gcctggatga aagagccgaa ctcatagata
     1081 gacttcacaa gggtgaaatc acgatccttt acgtatcgcc cgaaaccttt gtcggctgtt
     1141 cgctatctac catcattggc gacagaagaa tcggtctcat ggtcatagat gaggctcatc
     1201 tggtgacgac atggggaaag actttccggg tagattacgg ctatctggga gaagatctca
     1261 aggtcttgag agaaagaatg gactttccgg tgatggcaac aactgcgact gcaatttccg
     1321 gtggggagct ggacacagtt gatgagataa gcaacctcct ctacctcaga tcacccagaa
     1381 taatcatgac cagtgtcagg cgaaacaata tatccttcaa tatcacgaga ttcaatctct
     1441 ccgaagagaa gaaagaaagc agaacttctc aaaagatctc agatactgtg gattcaacga
     1501 tctctctgat agagtccggt aagaaaagca tagtatactg tcctttcgtc ggtcaggcgg
     1561 ataatatcta caccgttgtc agtagcgttg ccgaatgcag aaacaaagtt ggaaggtaca
     1621 ctggaccgac tggtgttgaa gaacgcagaa agactcaaag actctttggt aagggaatct
     1681 atcgaactgt gatagccaca aaggcatttg ggatgggcgt tgatatcccc gatattgacg
     1741 aagtatatca tcatagtgtg ccatcggtta tggcagacta cgtgcaggag attggaaggg
     1801 cggggagaga tggaagaccc tctattgcat ctacccactt caatgtcaaa gatctctct
//
LOCUS       DAOOLY010000377         1847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_80279_length_1847_cov_4.065290, whole genome shotgun sequence.
ACCESSION   DAOOLY010000377 DAOOLY010000000
VERSION     DAOOLY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80279_length_1847_cov_4.065290"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..825
                     /locus_tag="PLD15_12340"
     CDS             196..825
                     /locus_tag="PLD15_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64750.1"
                     /translation="MQVFVESVDKEVRLLDSTRHQLPHKKIIRIDELKEMGYSFYRIK
                     KLIEENILKKLTRKSYENLIYEGDESDFYYVNAYVPRGVICLISSAIYWQLTTERSTA
                     IDVAIPRSDRIYTMPEWPQIQLYYYSNKRYKTGIVTIHEDSNQFSIYDPEKTAIDILQ
                     YRERLGIEVAKEVLVQYLAKKDRNLNRLYRYAKELGNSKILRTYLEVLL"
     gene            822..1739
                     /locus_tag="PLD15_12345"
     CDS             822..1739
                     /locus_tag="PLD15_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPB64751.1"
                     /translation="MNKAASVKARLRNLARKQGRSYQDLLQIYALERTIYRLSLSPHK
                     DKFTLKGGIFLYALFEGRFPRSTTDIDLLGQRISNEKESLEKVFHDIFSVDTDDGMRF
                     DLESMNLRTIADAKQYPGTRITITAYIERTRLSVTVDVGFGDCITPERVQMEFPVLLN
                     DPEPVVFAYSKESVIAEKLEAIASLGFLTSRYKDFYDIFLLSRFFPFNGTTLQAAIGE
                     TFRNRGTPMEEIVAFEKQFISDSLHKRRWAAFAKKKNITFDTSLEEVMSQIKSFLIPV
                     LEALQRDEIFASHWEPDDLSWKFRRVEQR"
BASE COUNT          565 a          415 c          421 g          446 t
ORIGIN      
        1 ccctctctcc tctctcgtag ttcttgacca agaagctgga cacgaagtgt cggaacgaag
       61 aacagctctt ttgctcttgt tctccaagga cgggtccatg ctccgatacg aagtacgaag
      121 gacggctctt cgctctccaa ctaaatcact gcaattcatg atacaatttt aatgaacatc
      181 tgcatatacg taattatgca ggtgttcgtt gaaagtgttg ataaagaggt gagattattg
      241 gactcaacaa ggcaccaact acctcataag aagattatta gaatagatga actcaaagaa
      301 atgggatata gcttctatcg tatcaagaag cttatcgagg agaatatcct caagaaactg
      361 acgcgcaaaa gctatgaaaa tctaatctac gagggagatg aatcggactt ttactatgtt
      421 aatgcttatg tgccaagggg tgtgatctgc ctgattagct cggcaatcta ctggcagctt
      481 accacagagc gttccacagc aatagacgtg gcgattccca ggagcgacag aatttatacg
      541 atgccagagt ggcctcagat tcaactttac tattacagca acaagcgata caaaaccgga
      601 atcgtgacta ttcacgaaga ctcgaatcag ttctccattt atgacccgga aaagacagcg
      661 atcgacattc tgcaatatcg cgaaagactc ggcattgaag tcgcaaagga agtgcttgtt
      721 cagtatctcg caaaaaaaga tagaaacctg aacagactat acagatatgc caaggaactc
      781 ggcaattcaa agatactaag aacctatctg gaggtgttgc tatgaacaag gcagcatctg
      841 taaaagccag actgagaaat ctggcccgga agcaaggccg gagttatcag gatctactgc
      901 aaatctatgc gcttgaacga accatataca gattatccct ctctccccac aaagacaaat
      961 tcacattgaa aggcgggatc tttctttatg cgctatttga agggcggttc ccgcgatcaa
     1021 caaccgatat agatctgttg gggcagagga tcagcaacga aaaggaatct ctcgagaagg
     1081 ttttccatga tatcttctcg gtagataccg acgatgggat gcgtttcgat ctggaaagca
     1141 tgaacctacg caccattgca gatgcaaaac aataccccgg tactcgaata accattacgg
     1201 cctacataga gcgaacaaga ttatccgtaa ccgtcgatgt cggatttggc gactgtataa
     1261 cacccgagag ggttcagatg gagttcccgg tactgctgaa tgatccggag ccggtggtct
     1321 tcgcatactc aaaagaatcg gttatcgcag aaaaacttga agcgatagcc tcgctgggtt
     1381 tcctgaccag tcgctacaaa gacttctacg acatcttttt gctgtccaga ttctttcctt
     1441 tcaatggcac aacactgcaa gccgcaattg gggagacatt tagaaatcgt ggcacaccga
     1501 tggaagagat cgtcgcattc gagaagcaat tcatttccga ttcactccat aagagaagat
     1561 gggcagcctt cgctaaaaag aaaaacatta cgttcgatac atctcttgag gaagttatga
     1621 gccaaatcaa gagcttccta atccctgtac ttgaagcctt acagagagat gagatcttcg
     1681 cctcgcactg ggagcctgat gacctaagct ggaagttccg acgagtggaa cagaggtaag
     1741 aggttagagt ttagaggtaa gaaaaccaag atcttttcgc tcttcacgtc tgtttttcgc
     1801 gccatgaatc gagatcctgg ccagaagcat gccaggatga cttgatg
//
LOCUS       DAOOLY010000378         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_81015_length_1836_cov_3.156654, whole genome shotgun sequence.
ACCESSION   DAOOLY010000378 DAOOLY010000000
VERSION     DAOOLY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81015_length_1836_cov_3.156654"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..472
                     /locus_tag="PLD15_12350"
     CDS             161..472
                     /locus_tag="PLD15_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64752.1"
                     /translation="MNRGSITVLVISILLLCACAISVAFVLKSVRSFRDTSRLMVEAS
                     IRRGVLESAKKLLDFSVEEKCELYLEMNGYSLRTEFVNARWLVRIESGDFKKIIYAEG
                     R"
     gene            479..1366
                     /locus_tag="PLD15_12355"
     CDS             479..1366
                     /locus_tag="PLD15_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB64753.1"
                     /translation="MLAISIDSGCAPVKGFVEKHGLFFMGMKVIIDEKEYGDSFEFQE
                     NEFYKIVDSSRDFHTAQPPLGQVLEYYNDIKSKGFTQLLDIHFSSKMSGLYETCLMAS
                     KMVEGLEVFVVDTKKVSIGAFLVSKRLIELAKSGMEISRVLEEVPRAISNSFMEFSVP
                     TLKYLIKNGRIGKAQGLAGTLLNIKPILSVDEEGFITPIAKLRGMKKLQEQMVSNVVE
                     FLQERRKDVVLYSIYGSEEYRPMMEETRDRAIVALKETLGIRLEDLELINGRIWPTIA
                     CHSGPAVFGLACYGEKNSS"
     gene            1366..>1836
                     /locus_tag="PLD15_12360"
     CDS             1366..>1836
                     /locus_tag="PLD15_12360"
                     /inference="COORDINATES: protein motif:HMM:NF028499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HPB64754.1"
                     /translation="MLLENFLKCAEEVISLFEREGKEASLHAELVRLSSLIDPEEFDF
                     FPSLKEYLEKFLLYFSPLDSLPRDRRMTRIENGHKMIDKLREIFLVRSEEEVMSLLGK
                     PVAAATSIKYATSVGESRSKILKRMGIETAGDLVNYFPRDYEDRRTIVPLSSIAG"
BASE COUNT          498 a          357 c          497 g          484 t
ORIGIN      
        1 tgaagctagt atcgcttttc gaaaagacta ttcatgagca cggtgtcaaa atcgatttta
       61 ccggcgacag aataggggat ctgttcgtcg ttggctatgg gctggacttc gatgaagtgt
      121 accggaattt gccgtatgtg ggttatctga agtcggactt atgaacagag gaagtattac
      181 ggtactggtt atttcgattt tgcttttgtg tgcctgtgcg atatccgtcg cgttcgttct
      241 gaagagtgtt cgttcgtttc gtgatactag caggctaatg gtagaggctt ctatcaggag
      301 aggtgtactc gaatctgcaa agaaattgct ggacttcagt gttgaggaga aatgcgagtt
      361 gtatcttgag atgaacggtt actctctgag aacggagttc gttaatgcca gatggttggt
      421 tcggatagag agcggggatt tcaaaaagat aatatatgcc gagggtaggt gactcacttt
      481 gctggctata tccatagatt ccggatgtgc tccggtcaag ggattcgtag aaaagcacgg
      541 attgtttttt atgggtatga aagtgataat tgatgaaaaa gaatacggcg acagtttcga
      601 gttccaggaa aatgagttct ataaaatagt agattcttcc agagactttc ataccgcgca
      661 acctccgttg ggtcaggtgc tcgaatacta caacgacatc aaatctaaag gctttacaca
      721 acttctcgat atacattttt cctcgaagat gtcaggactt tacgaaacat gcctcatggc
      781 gtcaaaaatg gtagagggcc tggaggtgtt tgtggtggac accaaaaagg tctctatagg
      841 cgcttttttg gtttctaaga ggttgataga acttgccaaa agtggaatgg agatttccag
      901 agtgttggag gaagtgcccc gggcgattag caactctttt atggaattca gcgttccaac
      961 cctgaagtac ctgatcaaga acggtagaat agggaaggct cagggtcttg ccgggacgct
     1021 cctgaacata aagccgattc tctcagtgga cgaagagggt tttataactc ctatagccaa
     1081 attgagaggg atgaaaaaac tccaagaaca gatggtttcg aatgttgtcg agtttctcca
     1141 ggaaagaagg aaagacgtcg ttctctactc catatacggt agcgaggaat accgtcccat
     1201 gatggaggag acccgtgatc gggcaattgt ggctttgaag gagacgctgg gaataaggct
     1261 ggaggatcta gagcttatta atggtcgtat ctggcccacc atagcctgtc acagtggacc
     1321 cgccgttttc ggactggcct gttacgggga gaagaacagt tcataatgct cctggaaaat
     1381 tttctgaagt gcgctgaaga ggtaatttca cttttcgaaa gagaaggcaa agaggcttca
     1441 ctacacgcgg aactggttag actctcttct ctgatagacc cggaagagtt cgatttcttt
     1501 ccttctttga aagagtacct ggagaagttc ctgttatatt tctcacccct tgactctctg
     1561 cctcgtgaca gaaggatgac aaggatagag aacggccata agatgatcga caaactccgc
     1621 gaaatcttct tggtcagaag cgaagaggaa gttatgagcc tgctgggcaa acccgttgcg
     1681 gcagcgactt cgatcaagta cgccacctca gtcggtgaat caaggtcgaa gatactaaag
     1741 cggatgggaa tagagactgc gggagatctc gtgaactact ttccgcgcga ctacgaagac
     1801 aggagaacga tagtgccctt gtcctcgata gccggc
//
LOCUS       DAOOLY010000379         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_82428_length_1816_cov_3.057354, whole genome shotgun sequence.
ACCESSION   DAOOLY010000379 DAOOLY010000000
VERSION     DAOOLY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82428_length_1816_cov_3.057354"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLD15_12365"
     CDS             <1..201
                     /locus_tag="PLD15_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64755.1"
                     /translation="LTFTTIAPGARTIPVAISLFTGSISRQVPFGEIMAGAVVVTVPI
                     VVLVFIFQRRIVQGLTAGAVKG"
     gene            341..1609
                     /locus_tag="PLD15_12370"
     CDS             341..1609
                     /locus_tag="PLD15_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64756.1"
                     /translation="MRKLLLTLMVVLVALVAFSQIVLTVAAGAVGQELELTKQAAEAY
                     MSIHPDITVKVLDTPDMVQDRLGLYLQFLEAKSPEIDVYQVDVIWPGDLAQHFIDFYE
                     YNADKVVSMHFPAIVENNTVDGKLVAIPWFTDAGLLYYRTDLLEKYGLKPPTTWDELE
                     NAAKIIQEGERKTNPDFWGFVWQGNAYEGLTCDALEWLASNDAGTIISPDKKITIANA
                     NAIEILEKVAGWIGTISPSGVLTFAEEDARAVWQTGNAAFMRNWPYCYSLSKGPDSNV
                     AGKFDVSPLPAGKSGNGAATLGGWQLSVSKYSKNPKEAADLALFMAGYEIQKLRAVEG
                     SMNPTIQALYADYEVIKANPFFGSLFDVFINAVARPSTATAPRYNEVSTLFFKAVHRI
                     LSGTSDAQNVLEELALDLQDITGFEIVWGF"
BASE COUNT          506 a          425 c          458 g          427 t
ORIGIN      
        1 ctaaccttta caactatagc acccggagcg agaactattc cggttgctat ctcgctcttt
       61 accggaagca tctcgagaca ggttcccttt ggtgaaatca tggccggtgc tgtggttgtc
      121 accgttccca tagttgttct cgtcttcatc ttccagagaa ggatagttca gggcctcacg
      181 gccggggcgg tgaaaggttg aagatattcc ttgttttcga atcgcgcatg gtctgatggg
      241 aagacagggg ggtgatcccg gaagattcta gaacgaaagt gaaacactgt atgtgaaact
      301 agaaaagatc tttagaaact atttttaagg aggtattttt atgagaaagt tactgcttac
      361 cctaatggta gtgcttgtag cattggtggc gttttcacag atcgttctga ctgtggcggc
      421 cggagcggtc ggacaggagc tcgagctcac aaagcaggca gctgaagctt atatgagtat
      481 ccatcccgat atcacggtca aggttcttga cacccctgat atggtccagg atagacttgg
      541 actttatctg caatttctcg aagcgaagag ccctgagatc gatgtctatc aggttgacgt
      601 aatatggcct ggcgatctgg cacaacattt catcgacttc tatgaataca acgctgataa
      661 agtagtctca atgcacttcc cggcaatagt tgagaacaac acagtcgatg gtaaactcgt
      721 tgcaattccc tggttcaccg atgctggatt actctattac agaaccgacc tgctcgagaa
      781 gtacggactg aaaccaccca ccacctggga tgaacttgag aatgccgcaa agatcattca
      841 ggaaggcgaa agaaaaacca accccgactt ctggggcttt gtctggcagg gcaatgctta
      901 cgaaggacta acatgtgatg ctctcgagtg gctggcatcc aacgacgcag gtactattat
      961 cagccccgac aagaagataa caatagccaa tgccaacgct attgagatac tcgaaaaagt
     1021 cgccggctgg atcggcacca tatcgccatc aggagttctg accttcgccg aggaagatgc
     1081 aagggcagtg tggcaaacgg gaaacgcagc tttcatgagg aactggccct attgctacag
     1141 tcttagcaaa ggccccgaca gtaatgtcgc cggtaagttc gacgtttcac cgcttccggc
     1201 tggtaagagc gggaatggag ctgcaacgct tggaggttgg caactttcag tcagcaagta
     1261 cagtaaaaat ccaaaagaag cggcagatct agccttattc atggccggtt acgagattca
     1321 gaagctcaga gccgtcgagg gttcgatgaa cccgactata caggctctct atgcagacta
     1381 tgaagttata aaagccaatc ccttcttcgg atcactcttc gatgtcttta taaacgcagt
     1441 ggcgagacca tctacagcga cggctcccag atacaacgaa gtatccaccc tcttcttcaa
     1501 ggcagttcac cgcattctct ccggaacatc agatgcccag aacgtccttg aagagctggc
     1561 gctcgatcta caagacataa ccggctttga aatagtctgg ggattctgat acaatagcgt
     1621 tcgggatact ggccccggaa ggggccagta tgtttgttag gaagaatcta aagaggtgaa
     1681 gcggtgaaaa cagttaagac agttaaaaag aaccttcaag aatatgtatc gttcagcgaa
     1741 agctccgttc tggaagatat agaaagactt tcagaacaac tcagaggagc gcggatactg
     1801 cacataaacg ccacgg
//
LOCUS       DAOOLY010000380         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_82615_length_1813_cov_4.072810, whole genome shotgun sequence.
ACCESSION   DAOOLY010000380 DAOOLY010000000
VERSION     DAOOLY010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82615_length_1813_cov_4.072810"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..983
                     /locus_tag="PLD15_12375"
     CDS             240..983
                     /locus_tag="PLD15_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPB64757.1"
                     /translation="MVVGDNSRRFIEAYKTIEMYLRKAGNIPDDFSFSKALNEKAEHD
                     RLLRPYRSKLLEYAKLRNVIVHSRTPDGAAIAEPHDSVVEEIESIARMLLDPPKITSL
                     KGGEKVTTIEAKDPIGKALEIIKEKSFSQIPVYSGKEYRGLLTTNAVARWMASASEEE
                     RAFIYDCPVERVLSYAEENEDVRFVRGSTNLFEVLEAFGKAGESGKRLEAILVTDSGK
                     RDEKPTRIITIWDIPEINSRLEPVRNMRS"
     gene            988..1524
                     /locus_tag="PLD15_12380"
     CDS             988..1524
                     /locus_tag="PLD15_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64758.1"
                     /translation="MSWNWQEELKEIVRTAVERKLNNLVLESQWENSASIHGLVYTEP
                     VRREEILLCRRELDACKKSGLSFEQAREELIEKLSERLLKLLLEIKRMEMERLLPYTI
                     ISVSDLTDDLSTAHYVYLSRNPGDREPLGSSIREEMRKLSRKYPEQLPDGGHVILPRE
                     YDMTFIVGFINHFYGRKN"
BASE COUNT          555 a          372 c          452 g          434 t
ORIGIN      
        1 ctaaccccct tgacagccac tccatacgac tggtataata agatcgtaaa cataccgtca
       61 ttcacttctg tgattcactc actctctata ataacgtctt agacgcgatc tctcgcactc
      121 caggatcatg ggctgtaacg gctccgtagt gttttcttgc atattttatc tgtcgtggtt
      181 tcttttgcag atgcgttctt ttcaccgctt catttgctca aaagagaagg aggataaata
      241 tggttgtggg agacaattcc agaaggttca tcgaagctta caagactatc gaaatgtatt
      301 tgaggaaggc gggaaacatt cccgacgatt tcagcttttc gaaagctctt aacgaaaagg
      361 cagaacacga tcgtctactg agaccctaca ggagcaaatt acttgaatat gcaaaactga
      421 ggaacgtgat cgttcattcg aggacacccg acggtgctgc cattgcagag cctcacgaca
      481 gtgtcgttga ggaaatcgaa tcaatcgccc gaatgctact ggatcctcca aaaataacct
      541 ctcttaaggg tggcgaaaag gtcactacca tcgaggctaa ggaccccata ggaaaggccc
      601 tggagataat aaaagagaaa tccttctcac agatcccggt ttacagcgga aaagagtaca
      661 ggggactgct gacaacgaac gctgtcgcta gatggatggc cagcgcgagt gaagaggaaa
      721 gagcctttat ctatgactgt cccgttgaga gagtcctgag ttacgccgaa gaaaacgaag
      781 acgtcagatt tgtcagggga agcacaaacc ttttcgaagt actggaagcc ttcggcaagg
      841 caggagagag cggcaagaga ctggaagcca ttctcgtgac cgatagcgga aagagagacg
      901 agaaaccgac acggataata accatctggg acatcccgga gatcaacagt cgacttgaac
      961 cggtgaggaa tatgaggagc tgataaaatg agctggaact ggcaggaaga attgaaagaa
     1021 atagtgagaa cggcggttga gagaaagctg aacaacctgg ttctggaaag ccagtgggag
     1081 aactcggcga gtattcatgg gctggtctat acagaaccgg taaggcgtga agagatactg
     1141 ctctgcagaa gggaactcga tgcctgcaag aaatcggggc tgtccttcga acaggctaga
     1201 gaagaactga tcgaaaaact ctcggaaagg ctactgaaac ttcttcttga gataaagcgg
     1261 atggaaatgg agagattgct cccctacacg ataatctctg tctcggacct cacggacgac
     1321 ttgagcacgg cccattatgt atatctgtcg aggaatcccg gcgacaggga accgctcggc
     1381 tcttcgataa gagaagagat gagaaaactc tctagaaagt atccggagca gctccccgat
     1441 ggtgggcatg tgatccttcc cagagaatac gacatgacat tcatcgtagg gttcataaac
     1501 catttctacg ggaggaagaa ttagaagcgc aacaaaatca tgtaattttg ttctgaatgt
     1561 taactgtgat agactaattt taagagatgg gtcagtctca aattcacatg cagttattca
     1621 tatttcctat tgtagtgaaa ctgatcatac atataataac agttcgagct gacatgtttt
     1681 ggagtcgttt aatttttcta atagttctgt tgttgaatct cgagtaatta ttagataatg
     1741 accaggagtg gttttatgca aatacttatg aaaaagctat gatgacaaga cttgatctga
     1801 tgaattcgga tag
//
LOCUS       DAOOLY010000381         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_82810_length_1811_cov_2.391800, whole genome shotgun sequence.
ACCESSION   DAOOLY010000381 DAOOLY010000000
VERSION     DAOOLY010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82810_length_1811_cov_2.391800"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1811)
                     /locus_tag="PLD15_12385"
     CDS             complement(<1..>1811)
                     /locus_tag="PLD15_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB64759.1"
                     /translation="DPAAGKFRRLSLIFLAIALTIVVVVAFLYLTPSPLTGQGDINIT
                     ARLEYALTSSGSWKERFSAWENSIAQWFDSDNRLRIPFGTGIGTFQLYHLLYSPQVLA
                     SNPDYMIVWNNFKRTHNDYIQGLSEMGLIGFFFIIAMVTFLVLKFFKTLYSIDNKRDL
                     LLYGALGAGIFSFAAHSFFEFPIHMQPNLMLALFISALAMGKYFTGGSRKLDLSRTLL
                     AGLLIPLAGVIIFLKASAFLGEGYFRMGQTDQQYYQAYYNQAQSLDIEALQELQNGID
                     SFSGNYSYLADVGAYMEKKGNELKTKYPGASPIELLEAADHERLSEISRLKTEINNRL
                     RQYDTLMDRALQYYEGAIENFKRSNRIYPVLGKPLWYLAGLGMKSNHLEEAKNNPELM
                     FDVLTGEGEFTSDIIPEFKGSLEVIPLPEREIRTLPFREIVSHNKAAFSNLELANGLQ
                     LYFITQLQMILDAADYYESSVILFSERQTPRILGRLYSSVNSELKKYYNFIDARNSLI
                     QSAFGSPQEFKKIVFDTIDMAAEKALYWFDLAVRLLPGTWNRYPDWENVYLEYMTSIE
                     TVGRSLAEKSRLLLSVAERHVWAAENMGPDSPSDTLQ"
BASE COUNT          428 a          430 c          464 g          489 t
ORIGIN      
        1 tattgaaggg tgtcactggg tgaatcaggt cccatgtttt cggcggccca gacatgtctt
       61 tcggccaccg agaggagcaa ccggcttttc tctgcgagag aacgccctac ggtttctata
      121 gatgtcatgt attcgagata tacattttcc cagtccgggt aacggttcca ggttcccgga
      181 agaagtctga cagccaggtc gaaccagtat aaagcctttt cggcggccat gtctatggtg
      241 tcgaaaacta tcttcttgaa ctcttgggga cttccaaagg cgctttgaat gagcgagttt
      301 cgggcgtcta tgaagttgta gtatttctta agctcgctgt tgacgctgct gtaaagcctc
      361 ccgagtattc tgggcgtctg cctttcgctg aagagtatca ccgacgattc ataataatcg
      421 gctgcatcca gtatcatctg tagctgggtg atgaagtaca gctggagtcc gtttgcaagc
      481 tcgagattgc tgaaggcggc tttattatgc gatactatct ccctaaacgg cagggtcctt
      541 atctctctct ccggaagtgg aatcacttcc aaactgcctt tgaattccgg gattatgtcg
      601 gatgtgaatt caccctcgcc cgtgagaacg tcgaacatga gctccggatt gttttttgct
      661 tcttcgagat ggttggattt cataccgagt ccggcgagat accagagcgg ttttccaagc
      721 accggatata tcctgttcga tcttttgaaa ttttcgatag ctccctcgta atactgaagc
      781 gccctgtcca ttaaggtatc gtactgtcta agacggttgt tgatctcagt tttcaatctg
      841 gatatttcgc tcagtctttc atgatcggcc gcttcgagca gctcgatcgg actggccccg
      901 gggtatttgg tcttcaactc gttgcccttc ttttccatat aggcaccgac gtcggccagg
      961 tagctgtagt ttccggaaaa agagtcaatg ccattttgca attcctgaag cgcctctata
     1021 tcaagactct gggcctggtt gtagtaagcc tggtagtact gctgatctgt ctgccccatt
     1081 ctgaagtagc cttcacccag aaaagcggat gcttttagaa aaattatcac tccggccagt
     1141 ggaatcaaca gccctgcaag aagggttctg gaaaggtcga gcttccttga acctccggtg
     1201 aagtactttc ccatggccag ggcagatatg aaaagcgcca gcataaggtt tggctgcatg
     1261 tggatgggga attcgaagaa gctgtgggcg gcgaaggaaa agatccccgc gccgagtgcc
     1321 ccgtacaaaa gtagatctct tttgttatct atcgagtata gagttttaaa aaactttaag
     1381 actagaaagg tcaccatcgc gattatgaag aaaaagccta tcagacccat ctcactcagg
     1441 ccctgaatat aatcgttgtg agttctcttg aagttattcc atacgatcat gtagtcggga
     1501 ttgctggcta acacctgcgg gctgtagagt agatggtaga gctggaaggt acctattccc
     1561 gtaccgaagg gaattctgag tctattgtcg ctatcgaacc actgagctat cgagttctcc
     1621 caggctgaaa agcgctcctt ccaggagccc gaagaggtga gggcgtactc cagtctggcg
     1681 gtgatattga tatccccctg accggtcagc ggagaaggcg taaggtaaag aaaggccacc
     1741 actacgacta ttgtcagggc gattgcgaga aagattaagg acagtctcct gaatttcccg
     1801 gccgccggat c
//
LOCUS       DAOOLY010000382         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_84169_length_1792_cov_2.537709, whole genome shotgun sequence.
ACCESSION   DAOOLY010000382 DAOOLY010000000
VERSION     DAOOLY010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84169_length_1792_cov_2.537709"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLD15_12390"
     CDS             complement(<1..1379)
                     /locus_tag="PLD15_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPB64760.1"
                     /translation="MKTTMKKMNKVLDFEILVYLVMTLLIPLFVTKGFTHEPSTAKHF
                     FYVVGFALILLSVLIRKKRVDFEFNYVHMALLGIGVAALLSLITVAVDNPQYMRYTLE
                     VALYTLFLGLTAIYISNKFDTIEKIEITLLFFIIGASVVSIDALLNFYAGWDIFLGKV
                     GEPFARASARSTIGNPNFVSDYMGMTIPLVFYFIISRKPLAFFFKGFGGQLILKITMA
                     TLLAPMVAAVFVSQTRTVITAIFVGNVVFLLAYVFLKKNRKPELSDDPAAGKFRRLSL
                     IFLAIALIIVAVLAFLYLTPSSLTGQGDINITARLEYALTSSGSWKERFSAWKNSIVQ
                     WLDSNNRLRIPFGTGIGTFQLYHLLYSPQVLDSNPDYMIVWNNFKRTHNDYIQGLGEM
                     GLIGFVFIIAMVAFLVLKFFKTLLTIDNKRDLLLYGALGAGIFSFAAHSFFEFPLHMQ
                     PNLMLALFIS"
     gene            complement(1391..>1792)
                     /locus_tag="PLD15_12395"
     CDS             complement(1391..>1792)
                     /locus_tag="PLD15_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPB64761.1"
                     /translation="ITLHNFPEGMAVGVGAFTTEALVIAVAIGVQNIPEGAAVMASLV
                     GAGYKPTRALRISFLTGMVEVVGGLLGAILISVARPLLPYAMAFAGGAMLFVISDEVV
                     PETHSGGFERASTFSLIFGFIVMALLDNILG"
BASE COUNT          501 a          440 c          430 g          421 t
ORIGIN      
        1 gatatgaaga gcgcaagcat aagattcggc tgcatgtgga gggggaattc gaaaaaactg
       61 tgggcggcga aggaaaaaat ccccgcaccg agcgccccgt acaaaagtaa atctcttttg
      121 ttgtctattg tgagaagagt tttgaaaaac ttcaaaacca gaaaggccac catcgcgatt
      181 ataaagacaa aacctatcag acccatctca cccaggcctt gaatataatc gttgtgggtt
      241 ctcttgaagt tgttccatac gatcatatag tcggggttgc tgtcgagcac ctgcggactg
      301 taaagcaggt ggtagagctg aaaagttcct attcccgtac cgaaaggaat cctgagtctg
      361 ttgttgctat cgagccactg aacgatcgag ttcttccagg ccgaaaaacg ttccttccaa
      421 gagcccgaag aggtcagggc gtactcaagt ctggcggtga tattgatgtc accctgaccg
      481 gtcagcgaag aaggcgtcag gtaaagaaag gccagcactg cgactattat cagggcaatc
      541 gctagaaaga ttaaggacag tcttctgaat tttcctgccg ccgggtcgtc ggacaattcg
      601 ggttttctgt tctttttgag aaacacatat gcgagcagga agacgacgtt tccaacgaat
      661 atagcggtaa tgacggtcct ggtctgcgat acgaaaacgg cggccaccat aggggccagt
      721 agtgtcgcca tcgttatctt caagatcagc tgacctccaa accctttaaa aaagaaggcc
      781 agaggtttcc tcgatattat gaaatagaaa acgagcggta tcgtcattcc catatagtcg
      841 gaaacgaagt tcgggtttcc aatcgtagat ctggccgaag ccctggcgaa gggttcgcct
      901 accttcccca gaaagatatc ccaccccgcg tagaaattga gcaatgcgtc gatcgacact
      961 accgaagcgc ctatgatgaa aaaaagcagc gttatctcga tcttttctat cgtgtcgaac
     1021 ttgttggata tatatatcgc cgtcaatcct agaaagagtg tataaagagc gacttcgaga
     1081 gtgtatctca tgtactgggg gttgtccacg gctacggtta tcagagatag cagggccgcc
     1141 acccctatac ccagaagcgc catatgaacg taattgaatt caaagtccac ccgtttcttc
     1201 cttatcaaca cactcaaaag tatcagagca aagccaacga cgtagaagaa atgtttagcc
     1261 gttgaaggct cgtgggtgaa gcccttggtc acgaacagag gtattagcag tgtcatgacc
     1321 agatatacca gtatttcgaa gtccagcacc ttattcatct ttttcatcgt cgttttcatc
     1381 atctttcacc tcacccaaga atattgtcaa gtagagccat cactatgaag ccaaatatca
     1441 gcgaaaaggt agaggctctt tcaaagccgc cactgtgggt ctccggcact acttcatcgc
     1501 ttattacaaa gagcattgcg ccgcccgcga aggccatggc gtaagggaga agcggtctcg
     1561 ccacacttat gagtatcgct ccgagaagac ctccgacaac ctctaccatt cctgtgagaa
     1621 aggaaatcct caatgcacgg gtgggtttgt aaccagctcc aaccaggctg gccataacgg
     1681 ctgcaccttc gggaatattc tgtacgccga tagccacagc aattaccaga gcttcggtcg
     1741 taaaggcgcc gactccaact gccattcctt ccggaaagtt gtgcagcgtt at
//
LOCUS       DAOOLY010000383         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_85732_length_1770_cov_5.243732, whole genome shotgun sequence.
ACCESSION   DAOOLY010000383 DAOOLY010000000
VERSION     DAOOLY010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85732_length_1770_cov_5.243732"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLD15_12400"
     CDS             <1..257
                     /locus_tag="PLD15_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64762.1"
                     /translation="SKSEKTGGLRKKTARFWQPFLVASSVAIVFALEIAIFGLVPFVK
                     NPDTISLVMLSSLGAGFILMILAFLSGMVHDTEPDRRIAS"
     gene            254..748
                     /locus_tag="PLD15_12405"
     CDS             254..748
                     /locus_tag="PLD15_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPB64763.1"
                     /translation="MKEKFLVAYATKRGSTAEIAEKIGEVLRKRQMQVDVLPAREVVD
                     LSPYKTAILGTAVYIGLWRREAVKFLKTYADELAKLPLRIFLSGPTGAGDPLELLGGW
                     RFPESLRPTIERIKPVEITCFGGRIESDKMNPFERMIIKKVGAEIGDFRNWESIASWA
                     NGID"
     gene            complement(867..>1770)
                     /locus_tag="PLD15_12410"
     CDS             complement(867..>1770)
                     /locus_tag="PLD15_12410"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPB64764.1"
                     /translation="RLLEGVGVAPLTPMSADGTKIEYGAISNYVDFLVEKGVNGMFVL
                     GTTGEGTSLCVSERKKALEAFIKANDDRMTIISHCGAAVFEDILDLLKHSRACGAHAA
                     AVVTPFFFKHSQDELFAFYDAIATEMGEYPLYIYNIPSLTKNWISVDVLKRLHQKHPW
                     IVGVKDSSGDFVYALSVIQNLPGTFNTVVGCDAAYSAVLTNGGKACVSGPGAVFPEFF
                     VKVRDAVRAGKYEEAFKAQKSLTKLTMAVGNGANIPYMKSVLEKRGLKMGGVRMPLKV
                     STREEMEDLDRDLFRVMAEVGIQF"
BASE COUNT          419 a          462 c          468 g          421 t
ORIGIN      
        1 cttcaaagag cgaaaagacc ggcggccttc gaaaaaagac ggcccgtttt tggcagccct
       61 ttttggtggc ctcttccgtt gccattgtat ttgcgctaga aatcgccatc ttcggactcg
      121 tccccttcgt aaaaaacccg gatacgatct ctctggtgat gctttcttcg ctgggagcgg
      181 ggtttatact catgattctt gcctttctat cgggaatggt ccatgacact gaacctgaca
      241 ggaggattgc ctcatgaagg agaaattttt ggtagcctac gccaccaagc gtggatctac
      301 cgcggagatc gccgaaaaga tcggagaggt gcttcgaaaa agacagatgc aggtggatgt
      361 gctgcctgcc agggaagtcg tcgatctctc gccttacaaa acagcgatcc ttggaacggc
      421 cgtttatata gggctgtgga gaagggaggc ggtcaaattc ctgaaaacct acgcggatga
      481 gcttgcaaag ctgcccctgc ggatcttcct tagcggtccg accggtgcgg gagacccact
      541 ggaattgctt ggcggctggc gcttcccgga atctctgaga ccgacgatag agcgaataaa
      601 gcccgttgaa ataacctgct tcggaggaag aatagagagc gacaagatga atcccttcga
      661 aagaatgata atcaaaaaag tcggggccga gatcggtgat ttccgcaact gggagagtat
      721 cgcttcgtgg gcgaatggaa ttgactgaag ttgccagtga tgtttcgcgc gttagttgct
      781 tgaccggaga gcatcaaaaa gcggccggac atgaaacccg gccgctttcg attttctcgg
      841 tttttgagag tatcgaatgg ggaggctcag aactgtattc caacctcggc cataactctg
      901 aacaagtcgc gatcgagatc ctccatctct tctctcgtgg aaacctttag aggcatcctt
      961 actccaccca tcttcaaacc cctcttctca agaaccgact tcatgtaagg tatgttggcc
     1021 ccgtttccaa cggccattgt gagtttggtg agggatttct gggctttgaa ggcctcctcg
     1081 tattttccgg ctcttaccgc atcgcgaact ttgacgaaga actccgggaa gaccgctccc
     1141 ggaccggaaa cacaggcctt ccctccgttc gtgagaacgg ccgaataggc cgcatcgcaa
     1201 ccgacgaccg tgttgaacgt accggggagg ttctggatca cagacaaagc gtacacgaag
     1261 tcgccgctgc tgtccttcac accgactatc cagggatgct tctggtgaag tctcttcaac
     1321 acatcgacgc ttatccagtt cttcgtgagc gacgggatgt tgtatatgta gagcgggtac
     1381 tctcccatct ctgtagcgat agcgtcgtag aaagcgaaga gttcatcctg gctatgctta
     1441 aagaagaatg gagtgaccac ggcggcggcg tgagcgccgc aggctctgga atgcttcaga
     1501 aggtcgagaa tatcctcgaa aaccgccgca ccgcagtggg atattatcgt cattctatcg
     1561 tcgttggcct ttatgaaagc ctcgagcgct ttcttccttt ctgaaacgca gagagaggtt
     1621 ccctcgcccg tggtgccgag cacgaacata ccgttcacac ccttctccac gagaaaatct
     1681 acataattgg atatcgcacc gtactctatt ttcgttccat cggccgacat gggagtcagt
     1741 ggagcgacac ctacaccttc taaaagtctc
//
LOCUS       DAOOLY010000384         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_85776_length_1770_cov_2.610496, whole genome shotgun sequence.
ACCESSION   DAOOLY010000384 DAOOLY010000000
VERSION     DAOOLY010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85776_length_1770_cov_2.610496"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLD15_12415"
     CDS             complement(<1..833)
                     /locus_tag="PLD15_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPB64765.1"
                     /translation="MKAVEFTGIIPPVLSSFESDGKIYEKGIREIIKFTLPHVNGYYP
                     IGTYGCGPLMSIEERKRALEIILDEVNGKVPVVAHVGTADTVTTVELAKHAKKSGAQG
                     VGAICPYYSPHLPEDYLFSHFAALIDAVNEEEFPVYVYNNPHLSQNTVTPALLKRLAE
                     YGLRGLKDSSFDLVNFYFFQEAVTEYSDFNVIIGTEAIFAGAFDAGATGCVCGMANIY
                     PELLAEIYEAHKAGNKEEVQRLQRLILEVRQITKLGPTVPIMHAILRARGIDAGYSRS
                     P"
     gene            complement(858..>1770)
                     /locus_tag="PLD15_12420"
     CDS             complement(858..>1770)
                     /locus_tag="PLD15_12420"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPB64766.1"
                     /translation="KASFDNALMVMAALAASMNHLLHVPAIASEIGIEIDFDYIGEIN
                     DRTPQLCTVNPNGEHSMADFAHAGGVPAVLKQLGGLLNEDCTNVEGKTVRQIAEEVAY
                     IDEKVIRSVAHPVNETGGIVILKGNIARNNAVVKRSTIPAGMMKFSGPAMVYDGEEEA
                     TEAIEKGKIKEGDIVVIRYVGPKGGPGMRELHRVAGALKSIGEKVAVVTEGRFSGATG
                     GLAVGYLGPESAEGGAIGVVKDGDIIEIDIASRTINVLLPDEEIARRLSTFVPKKTTG
                     RAKLLDEYARRVGPVNNGAVRIRDLEQ"
BASE COUNT          379 a          489 c          379 g          523 t
ORIGIN      
        1 taaggacttc tcgaataccc cgcgtctata cctctcgctc tgagaatcgc atgcataata
       61 ggcacggtcg gcccgagttt cgttatttgc cttacttcca gaatcaatct ctgaaggcgc
      121 tgaacttctt ctttattacc ggccttgtgg gcctcgtaga tttccgcgag aagctcggga
      181 taaatgtttg ccattccgca gacacagcct gttgcgcctg catcgaaagc acctgcgaag
      241 attgcctcgg ttccgataat cacgttgaaa tcggaatact ctgtgactgc ttcctggaaa
      301 aagtaaaaat tcacaaggtc gaaagaactg tctttcagac ccctcaagcc atattcggcg
      361 agtctcttca gtagagcggg ggtaactgtg ttctgagaaa gatgaggatt gttgtaaaca
      421 tatactggaa actcctcctc attcactgca tcgattaacg ctgcgaaatg gctaaagaga
      481 tagtcttcgg gaaggtgagg agaataatag ggacatatcg ctcccacacc ctgcgcaccg
      541 gacttctttg catgctttgc cagttctacg gtcgtaactg tatccgctgt gcctacgtga
      601 gccactaccg gcaccttgcc gtttacttcg tccagaatta tctctagcgc tcttttcctc
      661 tcttcgattg acatcagtgg gccgcatccg tatgtaccga ttggatagta accgttgacg
      721 tggggtagag tgaacttgat tatttccctg attcccttct cgtaaatctt cccgtcactc
      781 tcgaaggaag agagtactgg tggaataatt ccggtgaatt ctactgcttt catttttccc
      841 tcctaaatac actcaatcta ttgctctaga tctcgtattc ttactgctcc gttgttgacc
      901 ggtccgactc ttcttgcgta ctcatctagg agtttcgctc tccccgtggt cttcttcggc
      961 acgaaagtcg agagacggcg agcgatttcc tcatctggca agagaacatt tatcgtcctg
     1021 cttgcgatat cgatctctat gatgtctcca tctttgacga ctccgattgc tccaccttca
     1081 gcagattccg ggccgagata acctacggcc agtccacctg tagctcccga aaaccttcct
     1141 tcagtaacaa ctgcgacctt ttctcctatg ctcttcagcg ctccggcaac cctgtggagc
     1201 tctctcattc ccggaccacc ctttggtccc acatatctta taacgacgat gtctccctct
     1261 ttgatcttgc ccttttcaat tgcttctgtc gcttcttcct caccgtcgta gaccattgcc
     1321 ggacctgaaa atttcatcat acctgcgggg atagtggatc tctttacgac agcgttgttt
     1381 ctggcgatat ttcccttcaa gataactatt ccgcctgtct cgtttacagg atgtgcaacg
     1441 cttcttataa ctttctcgtc tatgtaggct acctcttcgg ctatctgcct gactgtcttg
     1501 ccctctacgt ttgtacagtc ttcattgagc aagccaccaa gctgtttgag taccgccggc
     1561 acacctccag catgtgcgaa gtctgccatc gagtgttccc cgtttggatt cactgtacac
     1621 agctggggag tcctgtcgtt tatctcacca atgtaatcga agtcgatttc tataccgatc
     1681 tcactggcta ttgccggaac gtgcagtaga tggttcatcg aagcggccag tgcagccatt
     1741 accatcagcg cgttgtcgaa agatgctttg
//
LOCUS       DAOOLY010000385         1763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_86247_length_1763_cov_4.358899, whole genome shotgun sequence.
ACCESSION   DAOOLY010000385 DAOOLY010000000
VERSION     DAOOLY010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86247_length_1763_cov_4.358899"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLD15_12425"
     CDS             <1..308
                     /locus_tag="PLD15_12425"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPB64767.1"
                     /translation="TRKALVPVRKPGKLPFETLEIDYELEYGRATLQMHIDAIHKGER
                     VLIVDDILATGGTMKAIAEMIESCGGEVISILCLAELDFLKPREKLARYDVRTLISY"
     gene            321..1685
                     /locus_tag="PLD15_12430"
     CDS             321..1685
                     /locus_tag="PLD15_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPB64768.1"
                     /translation="MLKYDFSFAFKETLESGLIFDELLETGKSTSTIMEKVNSPEPGF
                     LRILGDDSILDEVKTLEDWLENFENFVVIGIGGSALGNQALHSSLNPLNWNNLSREKR
                     MKKARIFVLDNVDPDMVSSILGELDLKLTVFNVISKSGTTAECMSNYLIARGLLDKLG
                     LAASEHFIFTTDSEKGVLREIADREKIRTLTIPDDVGGRFSVLTSVGLLSALAEGIDI
                     DGLYEGARFGRERYLRDNVKSNPALVNALIHYAYMKKGYNISVMMPYSNRLYTLADWY
                     RQLWAESLGKKFDFNGRIVHTGQTPIKALGAIDQHSQVQLYNEGPKDKVITFIKLEDF
                     GNQLTVPFIHSDVEALSYLNGVELAELLNAELRGTSIALASNGIPNLTITFPQIDAFH
                     VGEFIISYEIQTAIAGSMLRINPYDQPGVELGKKLTYAFMGRRGYEEIRDRYEEKTSW
                     RFEL"
BASE COUNT          501 a          394 c          447 g          421 t
ORIGIN      
        1 atacccgaaa agctctggtt cctgtgagaa agcccggcaa acttcccttt gagacactgg
       61 agatcgacta cgaactagag tacggtaggg caactctaca gatgcatatc gacgcaatac
      121 ataaaggcga gagagtattg atcgtggatg atatacttgc gacgggtgga acgatgaaag
      181 caatagccga aatgattgag agctgcggtg gtgaagtgat ctcaatattg tgcctggccg
      241 aactggattt cctgaaaccg agagagaaac tcgctcgtta cgatgtgcgc actttaatct
      301 cttactgacg gaggttagta atgctgaagt acgatttttc ttttgccttc aaagagaccc
      361 ttgaaagcgg tttgattttc gatgaacttc ttgagacggg taaaagcacc tcaacgataa
      421 tggaaaaggt caattcgccc gaacctggtt ttctgagaat attgggcgat gattccattc
      481 tggatgaagt gaagacactg gaagactggc tggaaaactt cgaaaacttt gtggtgatag
      541 gtatcggtgg ctccgcgctg ggaaatcagg cccttcactc atcgctgaac cccctaaact
      601 ggaacaacct gagcagggag aaacgtatga aaaaagccag gatcttcgtg ctggacaacg
      661 tagatcccga catggtctcc tcgattctgg gcgagttgga cctaaagttg acggttttca
      721 acgtcatctc gaaatccggt acaacggcag aatgcatgtc taattacctt atcgcaagag
      781 gccttctcga caagctgggt cttgcggcaa gcgagcattt cattttcaca actgatagcg
      841 aaaagggcgt gttgcgagag atagccgatc gcgaaaaaat aagaacattg acgattcccg
      901 acgatgtcgg cggacgcttc agcgtgctga cttcggttgg acttctttcg gccctagcgg
      961 agggcataga tatcgacggt ctctacgaag gagccaggtt cggccgtgaa aggtaccttc
     1021 gggacaatgt caagtccaac ccggccctcg tgaatgcgtt gatccattac gcatacatga
     1081 aaaaaggcta caatatatcg gttatgatgc cttactcgaa caggctttat acgcttgcgg
     1141 actggtacag acaactttgg gccgaatctc tgggtaagaa gttcgatttc aacggcagaa
     1201 tagtacatac tggccagact cctataaaag ctcttggggc gatagatcag cattcgcagg
     1261 tccagcttta caacgaaggc ccaaaggata aagtgataac ttttataaag cttgaagact
     1321 ttggaaatca gctcacagtt ccgtttatcc attccgatgt cgaggcactg tcctacctga
     1381 acggcgttga actggccgag ctgttgaatg cagaactcag agggacttcg atagcgctcg
     1441 catccaacgg aattcccaac ctgacgatca ctttcccaca gatcgatgct ttccacgtcg
     1501 gggagttcat aatatcttac gaaatacaga ccgcgatcgc aggaagcatg cttcgaataa
     1561 acccttacga tcagccgggg gttgaacttg gaaagaaact tacttacgcc ttcatggggc
     1621 gcaggggtta cgaggaaatc agagatagat acgaggagaa gacctcctgg aggtttgaac
     1681 tatgaaagaa gacttgctaa aactcataga agaattcgat gttaccgaac tttactttct
     1741 agaggaaacc attccgacct ata
//
LOCUS       DAOOLY010000386         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_86644_length_1758_cov_3.643570, whole genome shotgun sequence.
ACCESSION   DAOOLY010000386 DAOOLY010000000
VERSION     DAOOLY010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86644_length_1758_cov_3.643570"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..>1758
                     /locus_tag="PLD15_12435"
     CDS             324..>1758
                     /locus_tag="PLD15_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB64769.1"
                     /translation="MYLERVAALLKEKSGKIDYSVPREWLPANYTGTVKLRDRYIFVD
                     PSEFGHSMVGEILKKKRSDVDYSQSLGRTRGENDNSWLKSAIVYGAFVRSTTAYPHFD
                     PELFSDLHSPEYSESGTFYKMILMLPYLSRMGFDTLYFLPVTRYSDAFKKGTLGSPYS
                     VKDFFSIDERYHDRLLGDMRVDEEFKAFVEAAHIMGMRVVLDFIPRTSARDSDLILDH
                     PDWFYWIDAAGLQKYGPPKIEGLGFAQASVENLEKIYSSSQVKKHLSLFRPSPDRIAP
                     ERWTNFVKASRGKKEFLEVLAAEFGVITPPGFSDWINDNQPTWDDITFLRLYRDHPVQ
                     SKKYVSADQPPYVLFDVIKSSFFPGAEPADDLWKSIESIMPFYNENFGVDGARLDMGH
                     ALPRELELRIIERAKSIDPSFAIIAEELVMGNDKKAADSGYDCILGNTWWMEPRVAEG
                     KLKELVYKVLPDLRIKTLASVETPDTPR"
BASE COUNT          427 a          440 c          485 g          406 t
ORIGIN      
        1 aaatcggtgg ggttgacgcc ccttgtgggt tcgagtccca ccgcctccgc cagtgcgact
       61 caatcgttga aagatggttg agtcttttta ttggaaggac cttcgctctt cattggagag
      121 ggtgcgagca agtcgcacct gcgatggaag gtggtagaat cttttagagg agattaacaa
      181 ctcctggaga gaattgtagt ccgtaaacgg aaatctttga actggaacgg ctcagaactt
      241 accgttactg gcgcggagtg ttaatgggag tctatgacaa tgaacggagg gcgttatcgc
      301 cctcattttt tggaggtggt atagtgtacc ttgagagagt agcggctctt cttaaggaga
      361 aaagcgggaa gatagactac tcggtacccc gtgaatggct acccgcaaat tacaccggta
      421 cggtgaaact aagagatcgc tatatcttcg tcgatccttc ggagttcggt cacagcatgg
      481 tgggcgagat tttgaaaaag aagcgatccg acgttgatta ttcacagtct ctgggaagga
      541 ccaggggcga aaacgacaac tcctggttga aatcggcgat agtatacggt gccttcgtca
      601 gatcgacgac ggcctacccg catttcgacc cggaactgtt ctccgatctc cattctccgg
      661 aatactcgga atctgggacc ttttacaaga tgatcctcat gcttccttac ctttccagaa
      721 tgggcttcga cacgctttac tttctgcctg tgacccgtta cagcgacgcc ttcaagaaag
      781 ggactctcgg gtcaccctat tcggtcaagg acttcttcag catagacgag aggtatcatg
      841 accgactcct cggtgacatg cgggttgacg aggagttcaa ggccttcgta gaggctgctc
      901 acataatggg gatgagggtc gtcctcgact tcattccgcg cacgtcggcc cgcgattccg
      961 acctgatact cgatcatccc gactggttct actggataga cgcggccggg ctgcagaaat
     1021 acggacctcc aaaaatcgag gggttgggct tcgctcaggc tagcgtcgaa aatctcgaaa
     1081 agatctactc ttcgtcgcag gtcaagaagc atctttcgct cttcagacct tctccggaca
     1141 ggattgcccc ggaaaggtgg accaatttcg tgaaggccag cagagggaaa aaagagtttc
     1201 tcgaggtcct ggcagcggaa ttcggcgtaa taacccctcc gggcttttcc gactggataa
     1261 acgacaacca gcctacctgg gacgatataa ccttcctgag actttacaga gatcatcccg
     1321 ttcaatcgaa gaaatacgtt tccgccgacc agccgccgta tgtgcttttc gacgttatca
     1381 aatcctcctt cttccctggc gccgaaccgg ccgacgatct ctggaagagt atagagtcga
     1441 tcatgccctt ttacaacgag aatttcggcg tcgatggcgc gaggctggac atgggacacg
     1501 ccctgccgcg tgaactggag ctgagaataa tagaaagggc gaagtcgatc gatccatctt
     1561 tcgcgataat cgccgaagag cttgtgatgg gaaacgacaa gaaggcggcc gactcaggtt
     1621 acgactgcat actcggcaac acctggtgga tggagcctcg cgtggcggag ggaaagttga
     1681 aggagctggt ctataaggtt ctcccggatt tgagaataaa gactctcgcg agtgtcgaga
     1741 caccggacac cccaaggg
//
LOCUS       DAOOLY010000387         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_87460_length_1747_cov_7.003546, whole genome shotgun sequence.
ACCESSION   DAOOLY010000387 DAOOLY010000000
VERSION     DAOOLY010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87460_length_1747_cov_7.003546"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /gene="folE2"
                     /locus_tag="PLD15_12440"
     CDS             complement(<1..699)
                     /gene="folE2"
                     /locus_tag="PLD15_12440"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPB64770.1"
                     /translation="MLILRDVQNERDKRNIRINMVGIKSIEYPIVVLDREFGTQHTVG
                     NFDLFVDLPTDFRGTHMSRFVEVLERHHKKITPRNMESILDDIRDSLRADVAHIKVQF
                     PYFIRKNAPISGSESFSSFGCSFSTTKDGEFDFVLGVKVPVMTVCPCSKEISDRGAHN
                     QRAEVFVSVRMNSLVWIEEIIEYVEKSASAPIFSLLKREDEKYITEHSFDNPRFVEDL
                     SREVVLLLQDDSRIN"
     gene            741..829
                     /locus_tag="PLD15_12445"
     tRNA            741..829
                     /locus_tag="PLD15_12445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:Ser,seq:tga)
     gene            complement(848..1690)
                     /gene="lepB"
                     /locus_tag="PLD15_12450"
     CDS             complement(848..1690)
                     /gene="lepB"
                     /locus_tag="PLD15_12450"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB64771.1"
                     /translation="MSAKKSEKASDKKGSRFLHEVKEWGKAILYAIVFGTIIRLFVFE
                     TMLVPTGSMIPTINPPARLFVEKITYEYRQPDYGDIVVFWTPYVDIESQKYLRAFDKF
                     MDFFAPAEYRGHVKYVKRLVGKPGDVLELKRAPDYTAANPVYQLYVNGDIPPSLEERR
                     YVREGVFYDPMFFFGLAHPDDPSVRFSPYYRLYQAYKGLIEYAEFYDTVLEPLGLEKY
                     IQQDPATGEVKVIVPEGFRFMMGDNSTNSFDSRYFGFVPEEAIIGSPMLTIWPLSDFG
                     PLRK"
BASE COUNT          459 a          452 c          354 g          482 t
ORIGIN      
        1 gttgatccga ctatcatctt gaagaagtaa gacgacttct cgcgaaagat cttcaacaaa
       61 tcgggggtta tcgaaagagt gttcggtaat gtatttctcg tcttctctct tgagaagaga
      121 gaagatcgga gcgctcgcag atttctcgac atactcgatt atctcttcta tccatacgag
      181 agagttcatt cgaacggaaa cgaatacctc ggctctctga ttgtgagctc ctctatcgct
      241 gatttcttta gaacagggac agacagtcat caccggaacc ttaactccga gcacaaaatc
      301 gaactctcca tccttcgttg tggaaaacga acagccaaac gagctgaaac tctcactgcc
      361 gcttataggc gcgttctttc ttatgaagta tggaaattgc accttgatgt gcgcaacatc
      421 tgccctaagt gaatctctaa tgtcgtccag aatcgattcc atgtttctcg gcgtgatttt
      481 cttgtgatgt ctttcaagaa cctcaacgaa tctcgacata tgtgtgcctc gaaaatccgt
      541 cgggagatcc acaaagagat cgaaattgcc taccgtatgc tgtgttccaa attccctatc
      601 caaaaccacg atgggatatt caattgactt tatcccgacc atgttgattc tgatgtttct
      661 cttgtctctc tcattctgta cgtctctcaa gatcaacacc ccttgaatct gctctttcga
      721 agaggtataa tcagctaagt ggaggcgtat cctaattggc taaggagccg gtcttgaaaa
      781 tcggtggggt tgacgcccct tgtgggttcg agtcccaccg cctccgccag aaccggggat
      841 tccccggtta ttttcttaat ggcccaaaat cagataatgg ccatattgtg agcataggac
      901 tcccaattat agcttcctct ggcacaaaac cgaaatacct gctgtcaaag ctgttggtag
      961 agttatcccc catcatgaat ctaaaccctt ccggaacaat aacctttact tctcccgttg
     1021 caggatcttg ctgaatatac ttttccagac cgagtggttc caacaccgta tcgtagaact
     1081 ccgcgtactc aataagcccc ttgtatgcct gatacagtct gtaatatggc gaaaacctca
     1141 ccgaagggtc atccgggtga gccaacccaa agaagaacat cggatcataa aagacccctt
     1201 ccctgacata tcgcctttcc tccagcgaag gaggaatgtc tccattaacg tacagctggt
     1261 aaactggatt agcagcagta tagtccggag ccctcttgag ttcaagaaca tcaccgggct
     1321 ttccaaccaa ccgcttcacg tatttcacgt ggcccctgta ttctgcgggt gcgaagaaat
     1381 ccataaactt gtcgaaggcc ctgagatact tctgagactc gatgtctacg taaggtgtcc
     1441 agaatacgac aatatctccg taatcaggct gtctgtattc gtatgtaatc ttctccacaa
     1501 ataacctggc gggaggattt atggtaggta tcatagaacc cgtcggaact agcatagtct
     1561 caaaaacaaa cagacgaatt atcgttccaa agacaatcgc gtaaagaatc gcctttcccc
     1621 actccttcac ttcgtggaga aagcgcgatc cttttttgtc agaggccttt tccgacttct
     1681 tagcagacac taggcctcag tcccttttct cttttatctt taccttaccc ttcacgtctc
     1741 taatgta
//
LOCUS       DAOOLY010000388         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_88296_length_1736_cov_4.046996, whole genome shotgun sequence.
ACCESSION   DAOOLY010000388 DAOOLY010000000
VERSION     DAOOLY010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88296_length_1736_cov_4.046996"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLD15_12455"
     CDS             complement(<1..313)
                     /locus_tag="PLD15_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64772.1"
                     /translation="MKRSLFLIIFFFFALFTLVLASDLTVEGLKGSVEKVVSGRFTYR
                     SENDQWLAGELETRRIKVFSPLGDYLTVALYINGAQYSRHEYKYDENGQKQETRYYKN
                     DA"
     gene            complement(369..1598)
                     /locus_tag="PLD15_12460"
     CDS             complement(369..1598)
                     /locus_tag="PLD15_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB64773.1"
                     /translation="MKIKKDAQFYRFAAYGFLKNLRFFDPFLILFFREMGLSFLEIGT
                     LISVREIATNILELPTGFIADLYGRRMSMVLSMVSYLASFMVFYLFPNFFVYMIAMVA
                     FAFGEAFRTGTHKAMILEYLRLNDMIDIKVDYYGATRAASQMGSAVNALIAAFLVFYS
                     GSYRIVFLASVIPYLLNLVNLSTYPKNLDGELKTSQKSETFRAFFGIFKNRDSFRGVL
                     NSSLYDAFFKVVQEYLQPILKAMALGLPVMVTLNPDQRTAVLVGTVYFIIYFATSYAS
                     KNAGNVSKKFGNEARALNYSYLLGAVLIIFSGLFQRLGIQLAAVAVFFLLFVLENFRR
                     PVNISYISDTIDHKTMASGLSVESQLKTLLMAILAPVIGFLADRLGVGLALILAGLLM
                     AALFFLVSLRSAGRKGS"
BASE COUNT          472 a          430 c          429 g          405 t
ORIGIN      
        1 cggcgtcgtt cttgtagtag cgtgtctcct gtttctggcc gttttcgtca tacttgtatt
       61 cgtgacgact gtactgggcg ccgttaatgt agagcgcaac agtcaggtaa tcgccgagcg
      121 gcgagaaaac cttgatcctt ctggtttcaa gttcaccggc cagccattga tcgttctcgc
      181 tccggtaggt gaaccttccc gacacgacct tttcgacact tcccttcaaa ccctcgacgg
      241 taagatccga agccaaaacc agggtgaaaa gggcaaagaa aaagaatatg atcaaaaaga
      301 gacttctctt catataaatc acctccctct tagattctat cagctacggg aagatagcgg
      361 gcacgatttc aactgccttt tcttccggcc gatctcaggc tcacaaggaa gaagagcgcg
      421 gccatcagga gaccggccag tatcagggcc agcccgacgc ccaacctgtc ggccagaaaa
      481 cctataacag gagccagtat ggccatcagg agcgttttca actgcgattc cactgatagc
      541 cccgaagcca ttgtcttgtg atcgatagtg tcgcttatat aacttatatt caccggtctt
      601 ctgaagtttt ccaggacgaa gagcaggaaa aagactgcga ctgcggccag ctgtatcccc
      661 agcctctgga agagaccgct gaagatgatc aatacggcgc ccagcaggta gctgtaattc
      721 aacgccctcg cctcgtttcc gaatttcttg ctgacgttcc cggcgttctt cgatgcgtag
      781 ctggtggcga aatagattat gaagtaaact gtgccgacaa gaaccgccgt cctctggtcg
      841 ggatttaaag taaccatcac aggcaacccg agagccatag ccttcaatat aggctgcaga
      901 tactcctgca caaccttgaa aaaagcgtcg taaagggagg agttcagcac gcccctgaag
      961 gagtcacggt tcttgaagat accgaagaag gctctgaagg tctcgctctt ctggctggtt
     1021 ttaagctcgc cgtccagatt tttggggtaa gtcgaaaggt ttaccagatt cagaagataa
     1081 ggtatcaccg aggccaggaa gactatcctg taactgccgc tgtagaagac caggaaggcc
     1141 gcgattaagg catttactgc agatcccatc tgtgaagcgg ctctggtggc tccgtagtag
     1201 tctaccttta tgtctatcat gtcgttcaat cttaagtact caagaatcat ggccttgtga
     1261 gtacccgtcc tgaaggcctc gccgaaggcg aaggcgacca tcgctatcat ataaacgaag
     1321 aagtttggaa agagataaaa gaccatgaac gaagcaagat acgacaccat ggaaagaacc
     1381 atagacatcc tgcgaccgta gagatcggct atgaacccag tgggaagctc gagtatgttg
     1441 gtggcgatct ccctcacgga aatcagagtt cctatctcca ggaaggagag acccatttct
     1501 ctgaagaaaa ggatcaaaaa gggatcgaaa aagcggaggt ttttcaggaa gccgtaagct
     1561 gcgaacctgt agaactgggc gtctttcttt atcttcaaga taactatctc ccggggaacc
     1621 tttatggcat atgataatta tagacctgcc atcgattccc cggaaggtat cttatttgat
     1681 gagaaagatc ctcaaaatca gataagatac cttccgggga atcgatggca ggtcta
//
LOCUS       DAOOLY010000389         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_89045_length_1727_cov_4.007177, whole genome shotgun sequence.
ACCESSION   DAOOLY010000389 DAOOLY010000000
VERSION     DAOOLY010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89045_length_1727_cov_4.007177"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLD15_12465"
     CDS             <1..833
                     /locus_tag="PLD15_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB64774.1"
                     /translation="DPLLMFDKFPGTAIPGFGMGVFGAAVATIISQTVAFILGFYIVF
                     SGREGVKPRLKGLFKLDWKIDKKLLTIGLPTGLESFFRNLSGAVLLKFVAVYGTAAVA
                     AVGVAGRLFGLAFMPLVGMNMGGSAMVGQNLGVDNVERARNTARTSALIGFSIMLLFV
                     LIAFFAGEIVLGIFNKDPEIVKYGTEFLRYGSLGISILAYGFGLSSVFGGSGYNMPFV
                     ISSIASRWFVQIPILFIMVHLLKASIVWVWLSYVFSDITEALVMFIYYRKGKWEKKRA
                     "
     gene            838..>1727
                     /locus_tag="PLD15_12470"
     CDS             838..>1727
                     /locus_tag="PLD15_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB64775.1"
                     /translation="MKRIAIVGAGIAGLSAGCYAQMNGFETEIFELHNNPGGLCTSWK
                     RKDYTIDGCIHHLPGASSSSKLYTMWKELGAIEDGRILFDSILRRTIDSGGNELNLYT
                     DLVRLKEHLMEISPADREAIDQYLKAAQKFTKFELLAMVSGGGKDKLSMIPHLPGIMK
                     WGPVSLEQFASRFKNSFLQKAFPTAQYDFQGIPMMIHLSFLAGCSIKSMGWPVGGSLA
                     FSKRIAKRYEELGGKIHYRSRVKSIAVKNDRAVGLTLEDRTKANADIVVSAADGYSTI
                     YDMLNGKYKDDFIDEYYANPP"
BASE COUNT          438 a          411 c          460 g          418 t
ORIGIN      
        1 tcgatccgtt gctcatgttc gacaaattcc cgggaactgc gataccgggc ttcgggatgg
       61 gtgtgttcgg agcggccgtg gcgactatca tatcgcaaac ggttgctttc atactcggct
      121 tctatatcgt cttttcggga agggagggcg taaaacccag attgaaaggg ctcttcaaac
      181 tcgactggaa aatagacaaa aagctcctca ccataggtct cccgacggga ctggagagct
      241 tcttcaggaa cctgtccgga gcggtgttgc tcaagttcgt cgcagtctat ggaacggcag
      301 ccgtggcggc cgtcggagtc gccggcagac tgttcggact tgccttcatg ccgctggtgg
      361 gaatgaacat gggaggatcg gcgatggtcg gtcaaaatct gggagtcgat aacgtcgaga
      421 gagccagaaa tacggccaga acatcggcct tgatcggttt ctcgatcatg ctacttttcg
      481 tcctgatagc ctttttcgcc ggcgaaatag tcctcggtat cttcaataaa gacccggaaa
      541 tcgtcaaata tggcaccgag tttttgagat acggttcact tgggatatcg atcctcgcct
      601 acgggttcgg tctctcctcg gtcttcggag gatcgggata caacatgccc ttcgtgatct
      661 cgagcatcgc ctcacgctgg ttcgtgcaga ttccgatcct gttcataatg gttcacctct
      721 taaaggccag tatagtttgg gtctggctct cttatgtctt ctcggacata accgaagcgc
      781 tggtgatgtt catctattac cggaaaggaa aatgggaaaa gaaaagggcg tgacaagatg
      841 aagagaattg ctatcgttgg ggcaggcata gccggactct ctgcaggctg ttatgcccaa
      901 atgaatggtt tcgagacgga aatcttcgaa ttgcacaaca atccaggcgg gctgtgtact
      961 tcgtggaagc gaaaagatta caccatagat ggctgtattc atcaccttcc aggtgcaagc
     1021 tcctcttcga agctgtacac aatgtggaaa gagcttggtg cgatcgaaga tggtcggatt
     1081 ttgttcgatt cgatcttgag aaggaccata gactccggtg gaaatgagct caacctgtac
     1141 accgatcttg tcagattgaa ggaacaccta atggagatct caccggccga cagagaagcc
     1201 atagatcagt atttgaaggc ggcacagaag ttcacgaagt tcgaattgct cgcgatggtc
     1261 agcggcggcg gtaaggataa actttcaatg attccacatc tgcccgggat aatgaagtgg
     1321 gggccggtat cgctagagca attcgcttcg agattcaaaa actccttcct tcaaaaggcc
     1381 ttcccgacgg ctcaatacga ctttcaggga atcccgatga tgattcatct ttccttcctt
     1441 gccggttgct caatcaagtc gatgggatgg cccgtcggag gttctctggc tttttcaaaa
     1501 aggatcgcga aacgttatga agagcttgga ggcaaaatcc attacagatc gagagtgaag
     1561 agcatcgccg tcaaaaacga cagggccgtc ggtcttacac tggaagacag aacaaaagca
     1621 aacgccgata tcgtcgtctc cgcagccgat gggtacagta ctatttacga catgcttaac
     1681 ggcaaataca aagacgattt tatcgacgaa tactacgcca atccacc
//
LOCUS       DAOOLY010000390         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_89574_length_1720_cov_3.591592, whole genome shotgun sequence.
ACCESSION   DAOOLY010000390 DAOOLY010000000
VERSION     DAOOLY010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89574_length_1720_cov_3.591592"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..718
                     /locus_tag="PLD15_12475"
     CDS             2..718
                     /locus_tag="PLD15_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HPB64776.1"
                     /translation="MATGTLGISPILSPIVSTSVTYSSAFLGGLLSFFSPCILPLIPV
                     FFCVLMGGAVDGKARFLRGVFFTLGMSLFFFVLGIGATGLGTFIRQNEVLMNVISGSL
                     FVLFGFLYLFEIGFKGVKLNVWKLGGSALSGFILGAVLGLVWVPCAGPILGSILVLAA
                     NQNTLGEGGIMLLVYSLGLSIPFLTLSGLVARITSRISFGGESRWRKVMRIAVFLILV
                     AAGILTMTGNLNLLQFATGG"
     gene            723..1397
                     /locus_tag="PLD15_12480"
     CDS             723..1397
                     /locus_tag="PLD15_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HPB64777.1"
                     /translation="MRRISVLLVLLIAVAAFSYVGMLDDFDTAMRLASIEKKEAIIMF
                     SDKNCSYCVKFEKETLADSNVQEILRAGFVFAQIYKSNSGKATFPTQGDVKEYSYSDL
                     YAAFGVRGTPTFWFYTTYGEPLTQLPGYVPASDFIPIVRFIGEKAYETVKFDDYRKLP
                     SEYVGTAALVKVNEEDYGFVLARDPLAREYNGEEVDPFTVWLTKDLATGQQLLERGAF
                     RVLVLN"
     gene            complement(1375..>1720)
                     /locus_tag="PLD15_12485"
     CDS             complement(1375..>1720)
                     /locus_tag="PLD15_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPB64778.1"
                     /translation="TGAGTLKVGKIIHVLGPRLGEKDIEEKLHGSFMNVYKKAGAMGI
                     VTLATPAISTGIFGVSVRICAREFFRATVDYFKSYPSSSLKLVKMCLINSKTYSEFLS
                     ISFDYFNSKQEL"
BASE COUNT          402 a          384 c          453 g          481 t
ORIGIN      
        1 agtggcaacg ggtacgttag gtatttctcc tattctcagt cctatagtca gtacgagtgt
       61 aacttacagc agtgcctttc ttggagggct tctgtctttc ttttctccgt gtattctccc
      121 gctgataccg gtgtttttct gtgttcttat gggaggagcg gtcgatggaa aggcgcgctt
      181 tctcagaggc gtgtttttca cgctcggtat gtctctcttc ttcttcgtgc tgggaatcgg
      241 agctaccggt ctgggaactt ttattagaca gaacgaggtt ttgatgaacg taatatctgg
      301 aagtcttttc gttcttttcg gattcctcta tctcttcgaa atcggtttta aaggcgtgaa
      361 actcaacgta tggaaactcg gaggatcggc tctcagtgga ttcattctcg gagctgttct
      421 cggactggtg tgggtgccgt gcgccggccc tatacttggc tcgatactgg tactggccgc
      481 caaccagaac acccttggcg agggtggaat catgttgctg gtatattcgc tcggcctgag
      541 cattccattt ctcacgctga gcggtttagt ggcgaggatc acttccagaa tttcctttgg
      601 aggcgagagt cgctggagaa aggttatgag aatagccgtg ttcttgatcc tcgtggcggc
      661 agggatactg acgatgactg gaaatctaaa tttgctgcaa tttgcaacgg gaggttgatt
      721 ttgtgagaag gatatctgtt ttactcgttt tactgatcgc tgtggcggct ttttcctatg
      781 taggaatgct cgacgacttc gatacggcaa tgaggctggc ttctatcgag aaaaaagaag
      841 cgatcatcat gttctccgac aagaactgta gttactgtgt gaaattcgaa aaggaaacac
      901 ttgcagacag taacgtacag gagattctaa gggccggttt cgtattcgct cagatataca
      961 aatccaactc cggtaaggct acctttccaa cccagggaga cgtaaaagaa tactcttaca
     1021 gtgatcttta cgcagccttc ggggtaaggg gaacgcccac tttctggttc tataccacct
     1081 acggtgagcc cctgacacag ctacccggat atgtgcctgc cagcgatttc atcccgatag
     1141 tgagattcat tggagagaag gcctacgaga ctgtgaagtt cgacgattac aggaaattgc
     1201 cttccgagta cgtgggaacg gctgcacttg tgaaggtcaa tgaagaggac tacgggtttg
     1261 ttctggccag ggatccgcta gccagagagt acaacggcga agaggttgat cccttcacgg
     1321 tctggctaac aaaagatctg gcaactggcc agcagctact cgagagaggc gccttcagag
     1381 ttcttgtttt gaattgaagt aatcgaagga tatcgagaga aattccgagt aagttttgct
     1441 gtttataagg cacatcttta ccagtttcaa cgatgaagat ggatagcttt tgaaataatc
     1501 aaccgtggcc cggaagaatt ctctcgcaca tatacgaacg gaaacaccga atatgcccgt
     1561 agatatggcc ggcgttgcca gggtgactat gcccatagcg ccggcctttt tatatacgtt
     1621 catgaaagag ccgtggagtt tctcttcgat atctttctca ccaagccgtg gcccgagaac
     1681 gtgtatgatc ttgccgacct tcagagtgcc tgcacctgtc
//
LOCUS       DAOOLY010000391         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_90360_length_1710_cov_4.743807, whole genome shotgun sequence.
ACCESSION   DAOOLY010000391 DAOOLY010000000
VERSION     DAOOLY010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90360_length_1710_cov_4.743807"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLD15_12490"
     CDS             complement(<1..802)
                     /locus_tag="PLD15_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPB64779.1"
                     /translation="MRSKLVLIVALVVLCVSALAVTYPTFDWTAKADEVPAARLMRDF
                     PVDYPETHPLVEGEFDYEHYFTYEEMVYWLQRWAEENPDIVDVYTVGESFLGIPIYQI
                     TITNKATGKDTDKPAAFFSGNRHSGEVTSSVVNMLFAWTLISNYGKDAEITALVDKFT
                     YYVRPTENPDGMQQYLTTPYTNRSTVRPMDNDGDGKFDEDPGEDLNGDGFIRQMRIYV
                     GEGKGNYIIDPRDPKGRVMTSVGTGKGDYLVQSEGWDWDGDGRTAEDGP"
     gene            complement(1329..>1710)
                     /locus_tag="PLD15_12495"
     CDS             complement(1329..>1710)
                     /locus_tag="PLD15_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPB64780.1"
                     /translation="EIAKDLEWVNTLFPRLKERKDQKGGTLSGGEQQMLAIGRALMSR
                     PDLLMLDEPSLGLAPVIVGEVFKVIRKVHQEGKTILLIEQNALGALKLADYAYVLETG
                     RIVLEGKGEELLNNEQVKKSYLGG"
BASE COUNT          409 a          462 c          395 g          444 t
ORIGIN      
        1 caggtccgtc ttcggcagtt ctgccgtcgc cgtcccagtc ccagccttca gactggacga
       61 ggtaatcgcc cttgccggta ccgacgctgg tcattaccct gcccttgggg tctctcggat
      121 cgattatgta gttgcccttg ccttcaccga cgtagatcct catctgtctg atgaagccgt
      181 ctccgttgag gtcttctccg gggtcttcat cgaacttgcc gtcaccgtcg ttgtccatcg
      241 gacggacggt ggatctgttg gtgtagggcg ttgtcaggta ttgctgcata ccatcggggt
      301 tctcggtcgg acggacgtaa taggtgaact tgtccaccag ggccgttatt tcggcatcct
      361 taccgtagtt gctgataagg gtccacgcga aaagcatgtt caccaccgaa gaagttacct
      421 ctccggagtg tctgtttcca gagaagaagg cggccggctt gtcggtgtcc ttaccggttg
      481 ccttgttggt gatcgttatc tggtagatgg ggatgcccag gaaagactcg ccgacggtgt
      541 agacatctac tatgtctggg ttctcttctg cccatctctg tagccagtaa accatttctt
      601 cgtacgtgaa gtagtgttcg tagtcgaatt cgccttctac gagcggatgt gtttctggat
      661 aatcgaccgg gaaatcacgc ataagccttg cagcaggaac ttcgtcggct ttcgcagtcc
      721 agtcgaaagt cggataagtc actgccagtg cggaaacgca caacacaacg agagccacaa
      781 ttaacacaag cttagacctc aagatggtcc acctccttgg ttaaacacaa cacagaaaaa
      841 tgttttggaa actttccctg tcacacactt tcacaagatc tttgcgtctg cctttggaac
      901 gatcaccccc tttgaaaagc ggatcttacg aagtgaccgt cagccgcttc acatatttgc
      961 cggacagaag actctgagga cttccatcac acgcatagat catatggaga tatcacattc
     1021 aagacacaag agactctcat gaatgagcaa tcgataaccg atatgctcga aggctaaagg
     1081 acagctgtaa gaagatcggg aaaccgaaaa gtgccaggat ttggcaagac tccaccaaaa
     1141 gatgtataag ctctccgtag cactggcaca gaattcacgg taatactcta tcagtaacat
     1201 aaattttctc aaccaacttg aattctgtgc cgatccccaa agtgaatcac attactcgat
     1261 aaagatttta gctttactca aaatttcgcc aggtgtttaa cttccgaaga ttatctctcc
     1321 ggacacaact aaccgcccag gtaagacttc ttcacctgtt cgttgttgag tagttcctcg
     1381 ccctttcctt caagaacaat tctcccggtt tcaagtacat aagcgtaatc ggccaactta
     1441 agagcaccca gagcgttctg ttctatcaag agaatggtct tgccttcctg gtgtaccttt
     1501 cgaataacct tgaagacctc cccgactatc accggtgcga gcccgagaga gggttcgtcg
     1561 agcatcagca ggtccggtct cgacatcaga gccctgccta tcgccagcat ctgctgttca
     1621 ccgcccgaaa gagtgccgcc tttctgatcc tttctctctt tcaaccttgg aaaaagcgtg
     1681 tttacccact cgagatcctt ggctatctcg
//
LOCUS       DAOOLY010000392         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_90522_length_1708_cov_5.104658, whole genome shotgun sequence.
ACCESSION   DAOOLY010000392 DAOOLY010000000
VERSION     DAOOLY010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90522_length_1708_cov_5.104658"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /gene="alaS"
                     /locus_tag="PLD15_12500"
     CDS             complement(<1..>1708)
                     /gene="alaS"
                     /locus_tag="PLD15_12500"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPB64781.1"
                     /translation="WAWEYVTEYLKLPENLLWVSIYLNDDEAFSIWHEEVGVPERKIV
                     RLGKDDNWWGPAGPTGPCGPDSEIFIDRGENDNCPDPDNCSPACNCGRFLEFWNLVFT
                     EYNQDEEGNLVYLEKKNIDTGFGLDRAASIMQNVNSNFDTDMFIPIIDRIQDILSVKY
                     GAQKERDVSIKVIADHARSIAFMISDGVLPSNEGRGYVLRRVLRRAVRHGSLLGYDRP
                     FLYRIVETVASHMGDIYPELRERLSFTQEIALKEEERFLSTLENGERRLRTMLVDKKE
                     LTGEELFSLHDTYGFPLELVEEMVEGTGVKLDREGYEAAMERQRERGREAIGDREYLG
                     NVQVYTELFDKTGNSVFSGYRTMRDRSTVMGLVKEGIEVDSLSQGETGEVVFSQTPFY
                     PEKGGQVTDRGTLERDDFLARVDYVFIPYQEMIIHRVTVEKGVLTTGQEVELIVDDSR
                     RRAVMRNHTATHLLHASLRKVLGEHVHQSGSLVLPDRLRFDFSHFQAMSEEEIKRVED
                     LVNWQILQAVPVNTTEVNFTEVKERDVMALFEEKYGDKVRVVTVNDFSKELCGGTHAG
                     NTG"
BASE COUNT          350 a          494 c          399 g          465 t
ORIGIN      
        1 cctgtgttgc cggcatgtgt tccaccacac agctctttgc tgaaatcgtt tacggtgacc
       61 actcttacct tatccccgta tttctcttcg aagagggcca tcacgtctct ttccttcacc
      121 tccgtgaagt tgacttcggt ggtgtttacc ggcacggctt gaagtatctg ccagttgacc
      181 agatcttcga ccctttttat ctcttcctcg ctcatagcct ggaagtgaga gaaatcgaac
      241 ctcaacctgt ctggaagcac cagcgaaccc gattgatgta cgtgctctcc gagcactttt
      301 ctcagagaag catgaagcag atgtgtagcc gtatggtttc tcataacggc tcgtcttctg
      361 ctgtcgtcaa cgatgagctc cacctcctga ccggtggtca gaacaccctt ctcgactgtc
      421 actctgtgta ttatcatctc ttgatatgga atgaaaacat aatcaaccct cgccagaaag
      481 tcgtccctct cgagagtacc cctgtcggta acctgtccgc ccttctcggg atagaagggt
      541 gtttgagaga aaacgacttc accggtctca ccctgcgaaa gcgaatcgac ttcgattccc
      601 tctttaacaa ggcccatcac cgtcgatcgg tctctcatgg ttctgtaacc ggagaacacg
      661 ctgttcccgg ttttatcgaa gagttccgtg taaacctgga cattgccgag gtattccctg
      721 tcgccgatgg cttcacggcc acgttctctt tgcctttcca tggcggcttc ataaccttcc
      781 ctgtctagct ttactccggt gccttccacc atctcttcca cgagttcaag aggaaaaccg
      841 taagtatcgt ggagtgagaa aagctcctcg ccggtcaatt cttttttgtc gacgagcatc
      901 gttctcagtc gcctctctcc gttttctagt gttgaaagaa accgctcttc ctccttcagt
      961 gctatttcct gggtgaaaga aagcctctcc ctgagctcgg gatatatatc gcccatgtgc
     1021 gaggcaaccg tttccactat cctgtaaagg aaaggccggt cgtatcccag gagcgagccg
     1081 tgacgcacgg ccctgcggag gaccctcctc agaacgtacc cgcgcccttc gttcgaggga
     1141 agcactccgt cggagatcat gaaagctatc gagcgggcat ggtcggctat gacttttata
     1201 gagacgtccc gttccttctg tgcgccatat ttcaccgaga gtatgtcctg aatcctgtcg
     1261 attatgggta taaacatgtc cgtatcgaaa ttggagttca cgttttgcat tatcgaggcg
     1321 gctctgtcga gaccgaatcc cgtgtctata ttcttctttt ccagataaac caggttcccc
     1381 tcttcgtcct ggttgtattc ggtgaagacg aggttccaga actccaggaa ccgtccacag
     1441 ttacaggccg ggctgcagtt gtcgggatcg gggcagttgt cgttctctcc gcgatctata
     1501 aagatctctg aatcaggtcc acacggcccg gtcggtccgg ccggtcccca ccagttgtcg
     1561 tcttttccaa gcctgacgat cttcctctca gggaccccca cttcctcgtg ccatatgctg
     1621 aaggcttcgt cgtcgttgag atagatagat acccagagca ggttttccgg aagcttgaga
     1681 tactcagtga cgtactccca ggcccatt
//
LOCUS       DAOOLY010000393         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_91937_length_1690_cov_4.192049, whole genome shotgun sequence.
ACCESSION   DAOOLY010000393 DAOOLY010000000
VERSION     DAOOLY010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91937_length_1690_cov_4.192049"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLD15_12505"
     CDS             <1..300
                     /locus_tag="PLD15_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64782.1"
                     /translation="ERSLEIAREMLKVMYLIDALEDYHSDRRHGDFNVLFGHEGPNLA
                     EYVESGVYSSLEAISLRGKFSTDRCLTEIIDSTFSFVVGRLALAREKYNKGVKDA"
     gene            293..1084
                     /locus_tag="PLD15_12510"
     CDS             293..1084
                     /locus_tag="PLD15_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPB64783.1"
                     /translation="MPDYYEVLGVRPDASDEEIKSKYRELVKMYHPDRHTNNPLQSLA
                     EQKMKEINEAYEVLGSPEKRRQYDGGNVGGASSRYSSSTAGSYEALIAQANGDLSQRL
                     WERAVQLCTKAIGMNPSRYEAYAIRGMARYQQNNHNSAIEDLNTAISRGGREEAIYAY
                     LAFSSAELGRHGEAISAINKAIDMGGRNPNYLAFLAIQYETIGRSEEARAAWDEVRRI
                     DPNNEMLRRRDQAWRVGGTYIDKKDAGTAAACATCVLLDLLFNCC"
     gene            1084..1689
                     /locus_tag="PLD15_12515"
     CDS             1084..1689
                     /locus_tag="PLD15_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64784.1"
                     /translation="MERALELISEYGYLLVFFGTVIDQSGIPVFIVAGGIFVAAGLLG
                     FWQVVLFSILALVVTDIPFIFIGRYLSRRLVGNYSTGKSVRWLKNFLIVGTAIFLKSP
                     TLFYLFSKVIPVIGKYVPVFAGFSSSRKARAVLLFSLGDVVYALVFTIGGVYLGDLFI
                     EYSRVLAVVIALLFAVFYLLAARIYGKRIKSRMGKERSDYR"
BASE COUNT          427 a          424 c          471 g          368 t
ORIGIN      
        1 gaacgaagcc ttgaaatcgc ccgggagatg ctcaaggtta tgtatcttat cgacgctctt
       61 gaagactacc attccgacag aagacacggc gatttcaacg tgcttttcgg tcacgaagga
      121 ccgaaccttg ccgaatacgt cgagagtggc gtatactctt cactggaggc tatttccctc
      181 cgtggaaagt tcagcaccga ccggtgcctt acagagataa tcgacagcac tttttccttc
      241 gtagtaggca gacttgctct cgcgagggag aagtacaaca agggggttaa agatgcctga
      301 ttattacgaa gtcctcgggg tcaggcccga cgcttcggac gaagagataa agagcaaata
      361 ccgcgaactg gtgaagatgt accatccgga tagacatacc aacaacccgc ttcagtcgct
      421 ggctgagcag aagatgaaag agataaacga ggcctacgaa gtactgggca gccccgaaaa
      481 acgccgccag tacgacggcg gcaatgtcgg tggagcctcc tcgaggtaca gctcctctac
      541 tgccggaagt tatgaagccc tgatcgcaca ggccaacggt gacttatctc aaagactctg
      601 ggaaagggcc gtccagcttt gcacaaaggc gatcggtatg aatccttccc gctacgaggc
      661 ctatgctata agaggaatgg cccgttacca gcaaaacaac cacaacagcg caatcgaaga
      721 tctgaacacc gccatatcca gaggcgggag ggaagaagcg atctatgcat atctggcctt
      781 cagcagtgcc gagctcggca ggcacggaga ggccatctcg gcgataaata aggctatcga
      841 tatgggcggg agaaacccga actatctggc ctttctggcc atccagtacg aaacgatagg
      901 ccgtagcgaa gaggcgagag ccgcatggga cgaggtaaga aggatagatc ccaacaacga
      961 aatgctgaga cgacgcgatc aggcatggag agtcggtgga acgtacatag ataagaaaga
     1021 tgccggaacc gccgcggcct gcgccacctg cgtcctgctg gacctgctgt tcaactgctg
     1081 ctgatggaac gcgccctgga actcataagc gagtacggct atctgctcgt cttttttgga
     1141 accgtaatcg accagtcggg gataccggtt ttcatcgttg ccgggggcat cttcgtggcg
     1201 gccgggctgc tcggtttctg gcaggtagta ttattctcga ttctggcact ggtcgtaacg
     1261 gacattccct tcatcttcat tggacggtac ctttcccgaa gattggtggg aaattactcc
     1321 accggaaaga gcgtaagatg gttgaagaat ttcctgatag tgggaacggc gatctttctg
     1381 aaatctccga cgctttttta tctcttctcg aaagtgatac cggttatcgg aaagtacgtg
     1441 cctgttttcg ccggcttcag cagctcccgc aaggccaggg ccgtactcct cttctcgctg
     1501 ggagatgttg tatacgcact cgtcttcacg ataggcggag tctatctggg agatctcttc
     1561 atagagtact ccagagtgct ggccgttgtg atcgctctac tcttcgcagt tttttacctg
     1621 cttgcggcaa ggatatacgg aaagaggata aagagccgga tgggcaaaga aaggagtgac
     1681 tatcgttgaa
//
LOCUS       DAOOLY010000394         1683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_92539_length_1683_cov_2.488943, whole genome shotgun sequence.
ACCESSION   DAOOLY010000394 DAOOLY010000000
VERSION     DAOOLY010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92539_length_1683_cov_2.488943"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1328)
                     /locus_tag="PLD15_12520"
     CDS             complement(15..1328)
                     /locus_tag="PLD15_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64785.1"
                     /translation="MKRKKGIILGVTLMMFLTIAVLGTTIMAIVMSKARQTNDLEKRT
                     QAYFFARSGVEIAIQYLIDKPSENDSQNKIWILHGNLPAPGGNSDFSISEKTREGEWY
                     SNVRNALEEFRNDEGNGEEVVTAVWQEAEKLNILSLGTASDVTRVVSYMGRYTIPETV
                     FPFALYATGSITAKGASTIIGKIGTPDINNVSGIDDENIIVVEEGMNYEYPEFKESPF
                     DETSPVTINPAVISSDGYYKGITGSITIKTDPTDPDSMRVGIDNLSNVSSIVIEGTGK
                     VELYVDDLLSPKGVINWDGDPGKLSIYYHHTVTKENEKDVGTIDLSAFKMSGTLFIYG
                     EHDLDLKKTPVITFGTSNDSGFKLNGLMVVDGVYKIYARGGDHGNNVATIICAPDSTV
                     DFGGNAEFYGGFVVKSFESKGTAVLSRRIESAENPIGIIIDTMSS"
BASE COUNT          415 a          413 c          306 g          549 t
ORIGIN      
        1 cactcgcccc acagttagct tgacattgta tctataatta taccgatggg attctctgcc
       61 gactcgattc ggcgcgataa aacagcagtg cccttgcttt caaaggattt aacaacaaaa
      121 ccaccataaa actcagcatt tcccccaaaa tcgacggttg aatctggtgc acaaataata
      181 gtcgctacgt tgttaccatg gtcgcctccc cgtgcgtaaa ttttataaac accatcgacg
      241 accatgagcc cattaagttt gaagccactg tcatttgaag tcccgaacgt gatcactggt
      301 gtttttttga gatccaggtc gtgttctcca tatataaaga gggttcccga cattttaaaa
      361 gctgatagat ctattgtccc cacgtctttt tcattttctt tagttactgt gtgatggtaa
      421 tagatagata gttttcctgg atctccatcc caatttataa ctcccttagg gcttaaaaga
      481 tcatcgacat acagttcaac cttaccggta ccctctatca caatactact tacattcgaa
      541 aggttatcta ttccaactct catagaatca ggatcagtag ggtcagtttt gattgtgata
      601 cttccggtaa ttcctttgta atacccatct gagctgatta ctgctggatt gatcgttacc
      661 ggagacgttt cgtcgaaggg ggattcttta aattctggat actcatagtt cataccctcc
      721 tctaccacta tgatattttc atcatctatt ccagagacat tattaatatc cggtgtacct
      781 attttgccta tgattgtaga agctcctttt gcagtaatac tacctgttgc atacagagcg
      841 aatggaaata cggtctctgg aattgtatat ctacccatat acgatacgac tctcgtgaca
      901 tcactagccg ttcctaaaga aaggatattc aatttttccg cttcctgcca cactgcggtt
      961 actacttctt ctccgttgcc ttcatcgttt ctgaattcct cgagcgcatt tctaacgttg
     1021 ctataccact ctccctctct ggttttctct gaaattgaga aatcggaatt cccgcccgga
     1081 gccgggagat ttccatgtaa aatccaaatt ttgttctgag agtcattctc ggatggtttg
     1141 tcgattaaat actgtatagc gatttcaaca ccggatctcg cgaagaagta ggcctgtgtg
     1201 cgcttttcca ggtcgttggt ctgtctggct ttgctcatga caatggccat tatggtggtt
     1261 cccagaacgg ctatcgtgag aaacatcatc agggtgacac cgagtattat tccctttttt
     1321 cttttcatct atgcgcctcc ggccgaaatc atcggccaag cttctcatta cttataattc
     1381 agcttctaga tacagataat tgtaaatagc aaattcatgc catacgtttc gaataaatta
     1441 taacagtagt tattataaat aaatagaacc gtgtacagtc gtttgcttcc ttcctctaaa
     1501 aacgtttgtt tgtattaggt tgcgaaaaca gggactgacg ggctcctggt tttcactcca
     1561 attttcgcgt ctctctttct ctctcttcct ctctcctctt ctcgctcttt cgaaggacgg
     1621 gtcttcctgc tcttccagcc tctcgcctct tacctctagc ctcaggttct ccaaggacgg
     1681 ctc
//
LOCUS       DAOOLY010000395         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_92620_length_1682_cov_2.784880, whole genome shotgun sequence.
ACCESSION   DAOOLY010000395 DAOOLY010000000
VERSION     DAOOLY010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92620_length_1682_cov_2.784880"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLD15_12525"
     CDS             complement(<1..195)
                     /locus_tag="PLD15_12525"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPB64786.1"
                     /translation="MKIALLGDIAFFGKHSITNDKKAVLEYFKEAAEFLSKFDIVVGN
                     LETPLFDGGKPYGHKSAHIKA"
     gene            complement(192..1571)
                     /locus_tag="PLD15_12530"
     CDS             complement(192..1571)
                     /locus_tag="PLD15_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64787.1"
                     /translation="MKSSYNKIYDKSPIWLQNILISMFGYKLYRQRYGKTYKKYFNEY
                     LNKDYSDIESEMEYQSRELQRLITHAVNNSPFYREFYRGIDTTSIKTVDDLKKLPILE
                     KEVLRENVEKIYTLKKEEGITGITGGTTGKSLVVVFTKDDFQKRMAYLDAFKARLGVR
                     NGMRRATFSGRQFTTHNQKKKIFWRYNFVLKQKFYSTFDMVSENLPLYIRDLNKFKPE
                     TINGFVSAIYDIAKFVEDNNIALDFKPIGIFTTSETLLPFQRELIERVFSCPVYDQYA
                     SAEGAPFITECKNRNLHYNLDTGIIEPLETDYGTEMLVTSFTSYGTPLIRYRIGDMIE
                     FSNRKCSCGNCHPLVERIEGRQVDYLYSKERGKVSLSHLADVIKGMPNCIIAMQFIQD
                     KIDEIEIKLVVDEKQYKEQHEHMILEEMKYRFGENMRFRFVKVNEIPKESSGKYRLIK
                     NSVGIGEGL"
BASE COUNT          426 a          355 c          303 g          598 t
ORIGIN      
        1 agctttaatg tgtgctgact tgtgaccata gggtttgcca ccatcgaata aaggagtctc
       61 caggtttccc accacaatat caaatttgct caagaactca gcggcttctt tgaagtattc
      121 caatacagct ttcttgtcat ttgtgattga gtgctttcca aagaaagcaa tatcgccgag
      181 aagagcgatt ttcataatcc ttctccaatt cctacactgt tttttataag ccgatatttg
      241 ccgctagatt cttttggaat ttcattaacc ttaacgaacc tgaacctcat gttttcgcca
      301 aatctgtact tcatctcttc cagaatcata tgctcgtgct gttctttata ctgcttttcg
      361 tcgacaacaa gcttgatttc tatttcgtcg atcttgtctt gaatgaactg catagcaatt
      421 atgcagtttg gcattccttt tataacatcg gcaaggtggg aaaggctgac ttttcctctt
      481 tctttactat ataaatagtc aacctgacgg ccctctattc tttctacaag cgggtggcag
      541 ttaccacacg aacatttcct attgctgaac tctatcatgt cgccgattct atatcttatc
      601 agaggtgtgc cgtaggaagt gaagctagta acaagcattt cagtaccata atcggtttcc
      661 agcggctcga taataccggt atccaagttg tagtgaaggt ttctgttctt gcactctgta
      721 atgaaaggtg ctccttcggc cgaggcgtac tgatcatata cgggacatga aaagactctt
      781 tcgatgagct ctctttgaaa tggaaggaga gtttcagagg tggtaaatat ccctattggt
      841 ttgaaatcaa gtgcgatgtt gttatcttct acgaacttcg ctatatcgta tatagcagaa
      901 acaaatccat taattgtttc tggcttgaat ttgttcaaat cccgaatata aagaggcagg
      961 ttttcggaga ccatatcaaa tgtcgagtaa aacttctgct tcaatacgaa gttgtacctc
     1021 caaaatatct tttttttctg attgtgcgtc gtaaattgcc ttccactgaa ggtggctctt
     1081 cgcattccgt ttcttacgcc cagtcttgcc ttaaatgcat ctagataagc cattcgcttt
     1141 tgaaaatcat ctttagtaaa cacaaccacc agggattttc ccgttgttcc tccggttata
     1201 cctgttatcc cttcttcttt tttcagcgta tagatctttt cgacgttttc tctgagaact
     1261 tctttttcaa gtattgggag ctttttaagg tcgtctactg tcttaataga tgtagtatcg
     1321 atacccctat aaaactctct atagaaagga ctgttattta cggcatgcgt gatcagtctt
     1381 tgtaattctc tgctttgata ctccatttca ctctctatgt ctgagtagtc tttgttcagg
     1441 tattcgttga agtacttctt gtatgtttta ccatatctct gtctgtagag cttatagcca
     1501 aacattgaga tcagaatatt ttgaagccat atcgggcttt tatcgtagat cttattgtaa
     1561 gaagacttca ttctatcacc tcgcttaatt aacacgaagt cgttgaggtt tctcgataca
     1621 agcttttcat tctattttgt aaaagaaaat atgtattcta cagttctttt tattccgtcc
     1681 tc
//
LOCUS       DAOOLY010000396         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_92880_length_1679_cov_4.500616, whole genome shotgun sequence.
ACCESSION   DAOOLY010000396 DAOOLY010000000
VERSION     DAOOLY010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92880_length_1679_cov_4.500616"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1558)
                     /locus_tag="PLD15_12535"
     CDS             complement(68..1558)
                     /locus_tag="PLD15_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64788.1"
                     /translation="MTRKKTFLVFFLLLVAFSVALSMEQINEVSKTNTVVIDGENITY
                     TVTAGLLPVADQNGKEMVRIFSVSYERLLEREDPSRPVTFAFNGGPGVAAMFLHFGAF
                     GPRVADRSGDGAEIPAPPYSLVDNPYTLLDVTDLVFVDPVGTGYSEVADGIDPQRFWD
                     VKEDVAVFAGFIQSYLDWSGRRRSPVYILGESYGGIRGSYLASYLQDFGIFPEGIIFI
                     SPVFDLGTIQWSSMDDTALALSIPTYTAAAWYHGKLPAYLMTDLQKAIDSSREWVEEE
                     YIVSLWKGSELSEERKWATASKLSDFTGIDAEVLYKRGLRVNAVEFATLLLEEEGRSL
                     SMYDARITAFGPYVGDSNDETMFALSGQLKTCANDYFKRELGYLTALPYRSGNAEVYL
                     NWNWESGRPAPVSPDSLNTGYPDASKALAQAITRAPYFKVFVASGRFDLECPYESVAY
                     SLNHLEIPQSLQKNITHKVYPGGHMIYFNPQTQKQLREDLLDFYGR"
BASE COUNT          381 a          436 c          437 g          425 t
ORIGIN      
        1 atgttattca attggagagt caaaagactc tccttttgtt tttgcgcgtg acttccttac
       61 cgtcagttca cctgccgtaa aaatccagca ggtcctcccg gagttgtttc tgggtctgtg
      121 gattgaagta gatcatgtgt ccgccgggat aaactttgtg ggttatattt ttctgtagag
      181 attggggtat ctccagatgg ttcagactgt aagccaccga ttcgtaggga cattccaggt
      241 cgaatcttcc gctggcgacg aagactttga agtaaggagc cctggttatg gcctgggcga
      301 gcgccttgct ggcgtcggga tatcccgtgt tgagcgagtc gggactgacc ggagccggtc
      361 tgccgctctc ccagttccag tttagataga cttccgcgtt gccgctccga tagggtaatg
      421 cagtaagata gcccagctct ctcttgaaat agtcgttggc acatgtcttt aactgaccgg
      481 aaagagcgaa catggtttca tcgttcgaat cgcctacata agggccgaaa gccgttattc
      541 tggcgtcgta catactcaag gatcttccct cttcttccag caggagagtg gcgaattcca
      601 cggcgttaac cctcaaacct cttttataaa ggacttcagc gtcgattcct gtgaaatcgc
      661 tgagcttcga agcggttgcc cattttctct cctcggaaag ctcgctgcct ttccacagtg
      721 agactatgta ctcctcttcc acccactctc tggatgaatc gatagccttc tggagatcgg
      781 tcatgaggta ggccggcagc tttccgtggt accaggcggc cgcagtgtag gtcgggatcg
      841 atagcgccag cgcggtatcg tccattgaac tccactgaat cgttccgaga tcgaagacgg
      901 gcgaaatgaa gatgataccc tccggaaaga ttccaaaatc ctgcaaatag ctggccaggt
      961 acgaaccgcg aataccgccg taactctcgc cgagtatgta cacgggcgaa cggcgtcggc
     1021 cgctccagtc gaggtaagac tggatgaaac cggcgaagac cgccacatct tcctttacat
     1081 cccagaaacg ctgtggatcg atcccgtcgg caacttcgct gtatcccgta ccaacggggt
     1141 caacaaatac cagatcggtt acgtccagca gagtgtaggg gttatctacc agactgtacg
     1201 gaggtgcggg gatctccgcg ccatcgccgc tcctgtctgc gaccctggga ccaaaggctc
     1261 cgaaatggag gaacatggct gcgacaccgg gaccgccgtt gaaggcgaaa gtcaccggtc
     1321 ttgaaggatc ttctctttcc agtaaccttt cgtatgaaac gctgaagatc cgcaccatct
     1381 cttttccatt ctggtctgcc acggggagaa gtcccgccgt gactgtgtat gttatgttct
     1441 cgccgtcgat tacaacggtg tttgttttac ttacctcgtt gatctgctcc atggatagag
     1501 ctacagagaa agcgaccaga agcagaaaga aaactaagaa cgtttttttc cgggtcatcc
     1561 taaacacctc ctttttgtat tatagtcttc cggtcttctt atagtagatg tttgcaattg
     1621 tacttaagag ccgagaagga gcgtcgagag ttcgcttcgc tcacgagaag acgaaagcg
//
LOCUS       DAOOLY010000397         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_93069_length_1677_cov_3.011097, whole genome shotgun sequence.
ACCESSION   DAOOLY010000397 DAOOLY010000000
VERSION     DAOOLY010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93069_length_1677_cov_3.011097"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..676
                     /locus_tag="PLD15_12540"
     CDS             323..676
                     /locus_tag="PLD15_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HPB64789.1"
                     /translation="MGIGELIKSADWKNEKHVPVIDIPEAVIPGTAFDVTITVGKEIA
                     HPNTIEHHIKWFDLYVKYDDGQLLVHLGHIELTPVTTQSKAVLSIKLEKSGTLIATSY
                     CNIHGLWESSRKVDF"
     gene            699..860
                     /locus_tag="PLD15_12545"
     CDS             699..860
                     /locus_tag="PLD15_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPB64790.1"
                     /translation="MMKYRCTICGYIYDPEVGDPDNGVDPGTSFENVPDDWVCPLCGA
                     SKDDFEPID"
     gene            931..>1677
                     /locus_tag="PLD15_12550"
     CDS             931..>1677
                     /locus_tag="PLD15_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPB64791.1"
                     /translation="MKDYVIAISGEAGQGINTIGDMLALSLFRNGYCVFTDKSYHSRI
                     RGGEYVYRIRISGMPLYSMRQEFDLVVSLSKSTTLSQIEYFHDETVLVFDSDSEHVTH
                     EEAGIPFREVKFPFKKVAKEAGEPRAQNVVSLGALLSLFRVRREIPEKLVREAFEGKE
                     EVTRANLLALSKGYEIALPVEVPAFQPLAREYFLMNGTEGTGLGAIAAGCKFLAAYPM
                     TPGTGVMTFLASNALKHSIVVEQAEDEIAAV"
BASE COUNT          453 a          373 c          426 g          425 t
ORIGIN      
        1 ctataggact gagaatagga gaaataccca acgtacccgt tgccactgcc gttaattcat
       61 tcatattacc cctcccgggt atattataaa ccattgcttt tttcttttca aagcaaatcc
      121 tattacaaag cgcgaaagct cgtttttcaa ctgtagttct ttgtaactat tccaaataga
      181 ttcactcctt tgacagtatg atataattat tctactgggc cggtgctaca gaatctgccg
      241 tagaaagcta attagagagt cgtttgtttt tttgatgtgt cttatatttt tgatcatagt
      301 tctataacaa ggaggggtct taatgggaat tggcgagctt attaagagcg ccgactggaa
      361 gaacgaaaaa cacgtaccag ttattgatat ccccgaggca gttattcccg gtacggcatt
      421 cgatgtaacg ataaccgtcg gtaaggaaat cgcgcatccg aacaccatcg aacaccacat
      481 caagtggttc gatctatacg tcaaatacga tgacggacag ctgctggttc atctgggaca
      541 tatcgagtta acaccggtca cgacgcaatc caaggcagtg ctcagcatca aactcgaaaa
      601 gtcaggaact ctgattgcaa cttcctattg caacattcat ggcctttggg aaagttccag
      661 aaaagtcgat ttttagtatc cagtacaagg aggttggtgt gatgaagtac agatgtacga
      721 tttgcgggta tatctatgat ccggaagtcg gagatcccga caacggagtc gatcccggta
      781 cgagttttga gaacgttcca gatgactggg tttgccctct gtgcggagcc agtaaagacg
      841 attttgaacc tatcgactga atcgtaaagc gggagtccag ctcccgcttt tttattagtc
      901 aggagtttcg aacgccctgg aggtgtcaac ttgaaagact atgtgattgc tatatctgga
      961 gaagcgggcc agggtataaa caccatagga gatatgcttg ccctgagcct tttcaggaat
     1021 ggctactgcg tcttcacgga caagagttat cattcaagaa tcaggggtgg agagtatgta
     1081 tatcgcataa ggatatccgg gatgcccctc tattcgatgc gacaggaatt cgatcttgtg
     1141 gtctctttga gcaaaagtac cactttgagc cagatagagt atttccacga tgaaaccgtg
     1201 ctggtctttg attccgattc tgaacatgtc acccacgaag aggcggggat acccttcagg
     1261 gaagtcaaat tccccttcaa aaaggtcgcg aaagaagccg gagagccgcg cgcacagaat
     1321 gtggtctctc taggagcgct tttgagcctt ttcagagtga ggagagagat ccctgagaag
     1381 cttgtgaggg aggctttcga gggtaaagaa gaggtgacca gggctaacct gctggctctg
     1441 agcaagggct acgaaatagc cctgcccgtg gaagtgccag ccttccagcc cctcgccaga
     1501 gaatactttc tcatgaacgg gacagaaggg accggtctgg gtgccatagc ggcaggttgc
     1561 aagttcctgg cagcctaccc catgactccg ggaacgggag tgatgacttt cctggcttcg
     1621 aacgccttga aacactcgat agtagtggaa caggccgaag acgaaattgc agccgtg
//
LOCUS       DAOOLY010000398         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_93591_length_1671_cov_3.352723, whole genome shotgun sequence.
ACCESSION   DAOOLY010000398 DAOOLY010000000
VERSION     DAOOLY010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93591_length_1671_cov_3.352723"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1210)
                     /locus_tag="PLD15_12555"
     CDS             complement(299..1210)
                     /locus_tag="PLD15_12555"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB64792.1"
                     /translation="MNRYVALILDLDGTLLNDNKEISSGNLEALNSLRLKGIPVGVFT
                     GRDFEMARGYLDLISAEGPHALQNGALVITGKGEIIGRTTVFPDLAHTVINMANVRGC
                     DIVLREASIKPPDWFRVAELRMNPYLPFLKAYSHRIEYIEDLHVAAEARELLQIDVTG
                     PHEILSSILKELSIKYPGGFSHSLIKTNDDTAFMEYYWNSIEDETLLEKVKKDCESWG
                     LLSLFGPGISKGKALDSFVEYHSVSPSEVIFIGDHLADIPLMRRVGMPVAVANAMDVV
                     KEESKYVTSSNSEDGVARAIAVFFADL"
     gene            complement(1236..>1671)
                     /locus_tag="PLD15_12560"
     CDS             complement(1236..>1671)
                     /locus_tag="PLD15_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB64793.1"
                     /translation="CMAGERVFIGQFIKGMEYREMKIAQLMSNVVLEQFGRGCFIDKT
                     PSPEDFRLAREGLVRSEDILSGGSYDLVILDEIFIAHFFGLISSSEILNLMNMRSEGT
                     ELVLTGRKAPQEIIDRADLVTEMVEIKHYYARGVQARKGIEF"
BASE COUNT          415 a          457 c          353 g          446 t
ORIGIN      
        1 cgtgatcttt gtctcccagc accccggcgt tgtggtaggc tattccaagc tcaagtgaaa
       61 gactcttgtc ttcgggattc ctggctacca gattctcgag gatttcaatc tccgtggcga
      121 cacataaaca cgaaaaaaac actgttagaa tcagtaccgt cactctcatt taacgacctc
      181 cagataaatt ctatcaagcg acaggcgaag gattcggtct tcgaacaact gtgagactcg
      241 atcgaatctt caccaggtta ctaatgttcg cgctacaaga atccatgacc ggtaggctct
      301 aaagatctgc gaagaaaacg gctattgccc tggccacacc atcctcggaa tttgaagaag
      361 ttacatactt actttcttcc ttcacgacat ccatggcgtt ggccacagcc actggcattc
      421 ccactctcct catcagcggt atatctgcca gatgatcgcc tatgaagatc acctcagatg
      481 gggaaacact atggtactcg acgaaactgt caagagcctt tcctttagaa attcccggac
      541 cgaaaagcga gaggagcccc caactttcgc aatctttctt caccttctcc aacagagtct
      601 cgtcttcgat cgagttccag taatactcca taaaagccgt gtcgtcgtta gtcttgataa
      661 gcgaatggct gaaacctcca ggatacttga tggataactc cttcaagatc gacgatagga
      721 tctcgtgagg acccgtaacg tcgatctgga gcagctctct agcttcggct gcgacatgga
      781 ggtcctcgat gtattcaatc ctgtgcgaat aagcctttaa gaagggcaaa tagggattca
      841 ttctcagctc agctactctg aaccagtctg gtggttttat actcgcctct ctcagaacga
      901 tatcgcaacc cctcacattt gccatgttga tcactgtgtg tgccagatcg ggaaagaccg
      961 tggtgcggcc aataatttcg ccctttcctg tgatcactag cgctccgttt tgaagagcgt
     1021 gcgggccttc tgcggatatc aaatcgaggt atccgcgggc catctcaaag tcccttccgg
     1081 taaagacacc gaccggtatt cccttgagtc tgagactgtt caaagcttcg agattcccac
     1141 ttgagatttc tttgttatcg ttcagaagcg tcccatccag atcaaggatc agtgcgacgt
     1201 acctattcat tttttatcct ccgttagact ccctgtcaga attcgattcc ctttctcgcc
     1261 tgaacccctc tggcgtagta atgctttatc tctaccattt ccgttaccag atcggcccta
     1321 tctatgattt cctgtggagc ttttcttcca gtcagcacca gttcggtgcc ctcacttcgc
     1381 atgttcatga gatttagaat ctccgaagat gagatcaatc cgaaaaaatg cgcgatgaag
     1441 atctcgtcga gaattaccag gtcgtaagat cctcctgaca gaatatcttc tgatctaacg
     1501 agaccttctc gtgcaaggcg gaagtcctcg ggagaggggg tcttgtctat gaagcaaccg
     1561 cgcccaaact gctccagaac aacgttcgac atcaattgag cgatcttcat ctctcgatat
     1621 tccataccct ttatgaactg accgatgaag accctctcgc ccgccataca t
//
LOCUS       DAOOLY010000399         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_93660_length_1670_cov_4.455728, whole genome shotgun sequence.
ACCESSION   DAOOLY010000399 DAOOLY010000000
VERSION     DAOOLY010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93660_length_1670_cov_4.455728"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..778)
                     /locus_tag="PLD15_12565"
     CDS             complement(53..778)
                     /locus_tag="PLD15_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64794.1"
                     /translation="MRNRSLDLNYKSFTRNRLTMFEYRRKPLLDQLERIKNDIEKLKP
                     QDASEKLQRPSWAKSTNVDKAPTLSKMSSFDERRYSDLQKRMEEVEEKLEALDIEEED
                     IELEAIEDEIKRFDTLQKEYLESLEKARKEFEIEWDLASIELKYSEKNYIHQLMSKLD
                     SLRKRQNEINSGKTQRELKKKQETTGATMGTRAVNTLEDQTTGVRQVAQKPATTRETP
                     KQPETGESQTIIRRRGGGQIKDD"
     gene            complement(1002..>1670)
                     /locus_tag="PLD15_12570"
     CDS             complement(1002..>1670)
                     /locus_tag="PLD15_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPB64795.1"
                     /translation="RLHPFTLEPEGPVPNFDYFEKPLPLEDCTSEECIRIFRGVSVML
                     SELLRLRGLYTENHSESVSRLARSIAEQFGRGEELSFNLEAAGRVHDIGMLSLYSDMI
                     VKPGKLTWYECKILHEHPVLGYELVKKVPFPRKVARAVLEHHERLDGSGYPLGLRGSQ
                     ISLEGRILAVAEVVAAMSHYRTYRVEHGTRMALKEISDNSGKLYDSDVVSACYRSFER
                     GFTF"
BASE COUNT          346 a          415 c          371 g          538 t
ORIGIN      
        1 tagcggtgag cgtgccctgg gaagctccct gctttagctg ggagagtcgt cactaatcgt
       61 cctttatttg cccgccgcct cttcttctaa ttattgtttg cgattcccct gtctcaggct
      121 gttttggagt ttctctggtg gtagccggtt tttgggcaac ctgtcttaca ccggttgtct
      181 gatcctctag ggtatttaca gctctggtac ccatcgtcgc gcctgtcgtt tcctgtttct
      241 tttttagttc acgttgagtc tttccgctgt tgatttcatt ctgccttttg cgcagagagt
      301 caagtttcga catcagttga tgaatatagt ttttttccga atattttagt tctatgctgg
      361 ccagatccca ttcaatttcg aactctttcc ttgccttctc cagtgattcg agatactcct
      421 tttgcagcgt atcgaaacgt ttgatttcgt cttcaatcgc ttccagttct atgtcttcct
      481 cctcgatgtc cagtgcctcc agtttttctt ctacttcttc cattcttttc tgaagatcgc
      541 tgtatctcct ttcgtcgaat gaactcattt tgctcaaagt aggcgcttta tcgacgtttg
      601 tcgacttagc ccagctgggt ctttggagct tttcgctggc atcttgaggc ttgagcttct
      661 caatgtcgtt cttaatcctc tccagctgat ctagcaatgg ctttcttctg tactcgaaca
      721 ttgtcagtct gtttctggta aaagatttgt agttaagatc cagagatctg tttctcatat
      781 gctctgctca tcctcctcca tcttcgctag agtaattata tacattggac aggccattcc
      841 acgcctttga atagtctcgt gtagaaatat ccgggacagg ccagggatct tacaaccacg
      901 tacgatattt actatagtaa aaacgataat accaggcgaa ggtttgaaca gctattgatt
      961 cttctcacag tttgcaaaac agtgaggaaa ctgaaggacg attagaatgt aaagcctctt
     1021 tcgaaggaac ggtagcaggc actgaccaca tcgctatcgt acagtttgcc ggaattgtcg
     1081 cttatttcct tcagtgccat tcttgtaccg tgttctaccc tgtaggttct gtaatggctc
     1141 atcgcggcga ccacttcagc aaccgccagt attctgcctt cgagactaat ctgcgatccc
     1201 ctgaggccca gggggtaacc ggatccgtcg aggcgttcgt ggtgttccag aacggccctg
     1261 gccactttcc gggggaatgg tacttttttg accagttcat atcccagaac agggtgttca
     1321 tgaaggattt tgcactcgta ccaggtgagt tttccgggct tgactatcat gtccgagtag
     1381 agcgataaca tacctatatc atgcaccctg cccgcggctt cgaggttgaa cgaaagttct
     1441 tcgcctctac cgaactgttc agctatagag cgtgctagtc tggagacgct ctcactatgg
     1501 ttttcggtgt acaaccctct caacctgaga agctctgaaa gcataaccga gacacccctg
     1561 aaaatcctga tacattcttc actcgtgcaa tcttcgagtg gtagaggttt ttcgaagtaa
     1621 tcgaagttgg gaaccggtcc ctctggttcg agagtgaagg gatgaagcct
//
LOCUS       DAOOLY010000400         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_94066_length_1666_cov_2.404718, whole genome shotgun sequence.
ACCESSION   DAOOLY010000400 DAOOLY010000000
VERSION     DAOOLY010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94066_length_1666_cov_2.404718"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLD15_12575"
     CDS             complement(<1..163)
                     /locus_tag="PLD15_12575"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HPB64796.1"
                     /translation="MNIDLHTHLLPGVDDGSSDVEESLKILERMKSGGVERVYLTPHL
                     YSPRTPTDIF"
     gene            complement(181..>1666)
                     /locus_tag="PLD15_12580"
     CDS             complement(181..>1666)
                     /locus_tag="PLD15_12580"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPB64797.1"
                     /translation="FSKTEIPGLLLAISKAGGLTGEASSEIKIVGQENQSYWYQLMEG
                     QDITLKNGQTVFVPGRERYVYITGEVASTGRYDFTAEERMTVMRTLIKAGGARETASD
                     ELRVITPSGQVIPVKLSELLSGKSDPTLETASTIVVPKKVARITVLGAVRNPGTYIFN
                     RDESSSLADVVAKAGGLTDQKNVDRILVQISNEIKEFTVASIEKRSDNLPDNTFVYVT
                     SLEAAYVTVLGDVPRPGRYSLEGTQPVSVAEIIATAGGLGDTVNTLSIVSADGKIKLL
                     NALKIEDLTRNFLQKRDTIIVLKNYDSYVSIIGDVKSPGIFSIGDYGELSLAELITMA
                     GGFNSLEFSRKVQIQNAAKTEELTVGPDNFLLLSTKELEPGSVVFVPYIQTQRVYVFG
                     EVLRPGVVKFLEGMTAIEAVIEAGGPTSYAVLGNALLFQDPQKEPLVLNLDQQKGSPV
                     KGNVKLVPGNIIYVPQSSIVNIKDIMSIVASSLSIVNSAVGIFK"
BASE COUNT          383 a          441 c          388 g          454 t
ORIGIN      
        1 caaagatatc tgttggagta cgcggtgaat aaagatgcgg agtcagatag actctctcta
       61 cgcccccgct cttcattctt tcgagaattt ttagtgattc ttccacatct gaactgccat
      121 catcgactcc cggcaataga tgggtgtgta gatctatgtt cacactccaa cctcactcaa
      181 tcacttgaag atcccgacag ccgagttcac tatactaagt gacgaggcga cgattgacat
      241 tatatccttt atgttgacga tcgatgattg cggaacgtaa attatatttc cgggaactag
      301 ctttacgttt cccttcaccg ggcttccctt ctgctggtcc aggttgagca caaggggctc
      361 tttctgtgga tcctgaaaga gaagcgcatt gccgagaaca gcgtagcttg tcgggccacc
      421 ggcttctata accgcttcta tagccgtcat tccctcgaga aatttcacca ctcccggtct
      481 gagaacctct ccgaagacat aaaccctctg tgtctgtata tatggaacga agaccacaga
      541 tcccggttcc agttctttag tggaaaggag aaggaagttg tccggtccga cggtcagttc
      601 ttcggtcttc gcagcgttct gtatctgcac ttttctgctg aactcaagac tgttgaagcc
      661 accagccatc gtgatcagtt ctgccagcga cagttctccg taatcgccga tagagaagat
      721 acccggggat ttcacatcgc ctattatcga cacataagag tcgtagttct tcaagacgat
      781 tattgtatcc ctcttctgca agaaatttct ggtgagatcc tcgatcttga gtgcgttcag
      841 aagcttgatc ttaccgtcgg ccgacacgat gctcaaggtg ttgacggtgt ctccaaggcc
      901 accggcagta gctattatct cggctaccga caccggttgg gtcccttcaa gggaatacct
      961 acccggcctt ggaacgtctc ccagcacagt aacatacgca gcttcgagcg aggtcacgta
     1021 aacaaatgtg ttgtcaggaa ggttatcgct cctcttctct atggaggcaa ctgtgaactc
     1081 tttgatttcg ttcgaaatct gaactagaat tctgtcaacg ttcttctggt cggtaagacc
     1141 accggccttt gcaacaacgt cggcgaggga tgaactctcg tcgcgattga agatgtacgt
     1201 tccgggattt ctgacggctc ccaaaacggt tattctggcg accttctttg gaaccacgat
     1261 cgtgctcgcc gtttcgagag tcggatcact ctttcccgaa aggagctctg aaagtttcac
     1321 gggtataacc tgtcccgacg gggtaataac ccttaactcg tcggaggctg tctctctcgc
     1381 tcctccagcc tttatgagag ttctcatgac cgtcattctc tcttcggcgg tgaaatcgta
     1441 tctacccgtc gaggcgactt caccggttat gtagacgtat ctttctctac cgggtacaaa
     1501 tacagtctga ccgtttttca gggttatgtc ctgaccttcc atgagttgat accagtaaga
     1561 ctggttttcc tgaccgacaa tcttgatctc cgaagaggcc tcaccggtca accctccggc
     1621 cttagaaatc gcaaggagca gaccggggat ctcggtcttt gaaaag
//
LOCUS       DAOOLY010000401         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_94204_length_1664_cov_3.605966, whole genome shotgun sequence.
ACCESSION   DAOOLY010000401 DAOOLY010000000
VERSION     DAOOLY010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94204_length_1664_cov_3.605966"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1049
                     /gene="fabD"
                     /locus_tag="PLD15_12585"
     CDS             117..1049
                     /gene="fabD"
                     /locus_tag="PLD15_12585"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPB64798.1"
                     /translation="MVAWLFPGQGSQYVGMCKGLYESCASIRDNLELSGRLLGFDMVD
                     IMQNGPEEVLTLTQNAQPAILAHSIAISDLLSDYGSRPNIVAGLSLGEFSALVVAGSL
                     SYSDALRLVRLRGLAMQEAISPGEGTMAAIVGLPEELVERICGEVSPEGDVIVANYNC
                     PGQLVVSGLTALVRKVVLLAQERGARRTVELSVSAPFHSKFLQPVGEVLKEFLKGIQV
                     RAPKIPVISNVTAEFFPDDPEKIKELLIVQAFSPVKWEQTIRKMLSIGVDRFVEVGPG
                     RVLNSFVKKIDRNAQVSSTDVENLAQLREIEISA"
     gene            1068..1316
                     /gene="acpP"
                     /locus_tag="PLD15_12590"
     CDS             1068..1316
                     /gene="acpP"
                     /locus_tag="PLD15_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB64799.1"
                     /translation="MEKTANIREKVITIVAQHLGVDAKSVCGDSRFVEDLGADSLDLV
                     DLVMELEDEFSIQINDSETGKFKTIDDVVSFINTLEEK"
     gene            1319..>1664
                     /locus_tag="PLD15_12595"
     CDS             1319..>1664
                     /locus_tag="PLD15_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPB64800.1"
                     /translation="MKRVVITGLGVISPIGNNLSEFWNSLKNGKSGIDWIEGFDTSQF
                     QVKIAGQVKNFDPLRYMDRKEVRRNARFVQLAIASAQEAFEDSRLDESVFDRERAGVI
                     YGVGLGGMDVLEE"
BASE COUNT          461 a          325 c          502 g          376 t
ORIGIN      
        1 gtaatggccg gtcaggtctg tgggatcata aaggagatca acaccgtaga agagttaatt
       61 ggaaaactga tggaagaggc caaaaaaaca atcgattctt tgggagggaa gggcttatgg
      121 tagcttggtt atttcccggt cagggtagtc aatatgtggg aatgtgcaaa ggactttacg
      181 agagctgcgc ttcgataaga gataacctgg agctttcggg acggctcctc ggattcgata
      241 tggtagatat tatgcagaac ggtccagaag aagtgctgac attgactcag aacgctcagc
      301 cggcgattct ggctcatagc atagcgattt ccgatcttct gagcgattac ggctcgaggc
      361 ccaatatagt ggccggactc tcgctcggag agttcagtgc gctggtggtt gccggatcgc
      421 taagttactc tgacgcgttg agactggtga ggttgagggg attggcaatg caggaggcaa
      481 tctctcctgg agaaggcact atggccgcga tagtcggttt acccgaagaa ctcgtagaga
      541 gaatctgtgg ggaggtttcc ccggagggag acgtgatagt ggcaaattac aactgccccg
      601 gacaattggt cgtctccggt ctgacggcat tggtgagaaa agtggtccta cttgcccagg
      661 agcgtggtgc gagaaggacg gtcgagcttt cggtaagtgc accattccat agcaaattcc
      721 ttcagcccgt cggagaagta ctgaaagagt ttttaaaagg catccaggtg agggctccga
      781 agatacccgt catatccaac gtgacggccg agttctttcc cgacgatccg gaaaagataa
      841 aggagcttct catagttcag gccttcagcc cggtgaagtg ggaacaaacg atcaggaaga
      901 tgctttccat cggtgtcgac agattcgtgg aagtcggccc tggaagagtg ctcaattcat
      961 ttgtgaagaa gatagacaga aacgcacagg tgagttctac ggatgtggaa aatctggctc
     1021 aactgcgtga gatagagata agcgcataac aaaagaggtg aattactgtg gagaagactg
     1081 caaatatcag agaaaaggta attactatcg tggcacagca cttgggagtg gatgcaaaaa
     1141 gcgtgtgtgg agattcgaga tttgtcgaag atcttggagc agattcgctg gaccttgtcg
     1201 atctcgtaat ggagctggag gatgagttct ctatacagat aaacgatagt gaaacgggaa
     1261 agttcaagac catcgacgat gtagtatcgt tcataaacac gcttgaagag aagtgaaaat
     1321 gaaaagagta gtaattaccg gcctgggagt catcagtcca ataggcaaca atctttctga
     1381 attctggaac tctctgaaga acggtaaatc ggggatcgac tggatagagg gcttcgatac
     1441 ctcccagttt caggttaaga tcgccggaca ggtgaagaac ttcgatccat tgaggtacat
     1501 ggacagaaag gaggtccgaa ggaacgcaag gttcgtacag ctggcgattg ccagcgcaca
     1561 ggaggccttc gaagatagtc gtctagatga aagcgtattc gacagggagc gcgccggcgt
     1621 gatctacgga gtcggtcttg gagggatgga tgttttagag gagc
//
LOCUS       DAOOLY010000402         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_95885_length_1646_cov_4.695789, whole genome shotgun sequence.
ACCESSION   DAOOLY010000402 DAOOLY010000000
VERSION     DAOOLY010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95885_length_1646_cov_4.695789"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLD15_12600"
     CDS             complement(<1..353)
                     /locus_tag="PLD15_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPB64801.1"
                     /translation="MKKLYVVVDTETTGSKPLEGDRILEIAAIPVYGGKIMHNLAFQA
                     LVNPLVVIPAQITGIHGLRNEDVSEEPPMAEVFPKFKDYIGTSVLVGHNIVNDMTFFD
                     IASKETGLMPLSNNY"
     gene            441..758
                     /locus_tag="PLD15_12605"
     CDS             441..758
                     /locus_tag="PLD15_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HPB64802.1"
                     /translation="MKELRVHVVHKLGLSEARRRLEEKMGELMEQYEGKFTLESTWKD
                     NTLLFKLAAMGVKTEGTLEITDTTVTLAGTVPLVAIAFKKRIEESVKRDLEEILRVST
                     TPG"
     gene            831..1571
                     /locus_tag="PLD15_12610"
     CDS             831..1571
                     /locus_tag="PLD15_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPB64803.1"
                     /translation="MIDLHNHSNFSPDSNTPQSEIVERAIEKNLQVIGISDHDDLDTS
                     FPYSYRLKDPAGYLESLSLLKRNSPIKLLSGLEVGIGSSCNTPPEGEFDYFIYSVHSI
                     PGIDDLEIDNIWTLYLEESLDAIKGLTGPGFFGHIDFLRRYIKGHRELDNPVLLEELL
                     RRLVKSEIGIEINTSGWRAPYNESTPQPWIVERYLQMGGRYITIGSDSHRACDVGSHV
                     EKALSLLLDMGVREIFYCEKGTYMPLKI"
BASE COUNT          441 a          406 c          414 g          385 t
ORIGIN      
        1 atgtaattgt ttgaaagcgg cataagaccg gtttccttcg atgctatatc gaaaaaggtc
       61 atatcgttga ctatattgtg acccaccagc accgatgttc ctatataatc cttgaacttt
      121 ggaaagacct cggccattgg gggctcttca gagacgtctt cgtttctcag accatggata
      181 cccgttatct gcgctggtat gacgaccaga gggttgacca gagcctgaaa ggccaggttg
      241 tgcattatct tgccaccgta aaccggtatg gccgctatct cgagaatcct gtcgccctct
      301 agcggtttcg acccggtagt ctctgtatca acaactacgt aaagcttttt catcagaaaa
      361 cctcctgaaa aaattctacc atgagaaaat atacatacct taagttcatt tttcgagtag
      421 aattgtttga ggtgatctat ttgaaagagc tacgtgttca tgttgttcac aaactgggac
      481 tctccgaggc cagaagaagg ctggaagaga agatgggcga actcatggag cagtacgagg
      541 gcaaattcac gctcgaatcg acctggaagg acaacaccct cctgttcaaa cttgccgcga
      601 tgggtgtgaa aacggaaggg acgctcgaga taacagacac caccgttacg ctcgcgggaa
      661 ccgttccact ggttgcgatc gccttcaaaa agaggataga agagtccgta aagagggatc
      721 tggaagagat actgagagtg tcaacgaccc ccggctaaag ccgggagctt gtagctcagg
      781 ttgactagcc taaatgaaaa ccctgagcat cggtcttatc ggaggcgttc atgattgatc
      841 ttcacaacca cagcaacttc tctcccgaca gtaacacgcc ccagagcgag atagtcgaaa
      901 gagccatcga aaagaatcta caggtgatcg gcatatccga ccacgacgat ctcgacactt
      961 cctttcccta cagttacagg ctcaaagacc ccgccgggta tctggaaagc ctctcgctct
     1021 tgaaaaggaa ctcccctatc aagttgttga gcggactgga ggtcggcatc ggttcttcct
     1081 gcaacactcc gcccgaagga gagttcgatt acttcatcta ctccgtccat tcaataccgg
     1141 gaatcgacga tctggagatc gacaacatct ggacgctcta cctcgaggag tctctggatg
     1201 ctataaaggg gttgaccgga ccgggtttct tcggacacat cgactttcta cggaggtata
     1261 taaaagggca cagagaactc gataaccctg tgctcctcga ggagcttttg agaagactcg
     1321 tcaagtcgga gatagggatc gagataaaca cctctggctg gagggcgccg tacaacgaat
     1381 ccactcccca gccatggata gtcgagagat acctccagat gggcggaagg tacattacca
     1441 tcgggtcgga ctcgcacaga gcgtgcgacg tgggaagcca cgtggaaaaa gcgctttcac
     1501 tgttgctcga tatgggggta agggagatct tctactgtga gaaaggcacg tatatgcctc
     1561 tcaagatcta gatttagcgt tttttgtgcc ttttgagaga aaattttagg aagcgtttca
     1621 gattcatccc ctcgccaagc ctcagc
//
LOCUS       DAOOLY010000403         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_96677_length_1637_cov_3.786979, whole genome shotgun sequence.
ACCESSION   DAOOLY010000403 DAOOLY010000000
VERSION     DAOOLY010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96677_length_1637_cov_3.786979"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..>1637
                     /locus_tag="PLD15_12615"
     CDS             121..>1637
                     /locus_tag="PLD15_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPB64804.1"
                     /translation="MDKILAGAIGSDIHTAGILNFLGLARKEGYECVYIGSVLGIDKL
                     LDSIKEVSPDIIAISYRLGGESCRELLRELRTALEREGVEKKMIFGGTVETAGVARES
                     GMFDRVFDGSESLEEVTGYLRGRDEAGGTVEYPQGLLERIAFKEPFPLIRHHIGLQTL
                     EETELAVKELAQSRLLDIISLAPDQNCQQWFFHPENMVRSEDGAGGAPFRKREDFERM
                     YEASRRGNYPLVRSYSGTRELLKFSLLFKETLNNAWAAIPLTWYSQLDGRSDRELLEA
                     IRENQSAIAWNGGRGIPVEINEAHQWALRYCHDAIEVTMAYLAAMAARRLGVKVYVAQ
                     YMMNTPPGISPSMDIAKSLAKKELIESLKSESFIPVTMVRPGLMSFPADPHMSMGQLV
                     SSLFTASYLQPQIVHVVAYCEATKRATPVEIIESVKMVKHAMSEAAKGLPDFSKDDKI
                     ERHKEFLKNESATILEAMESIKTSSLTSPETIYAAINAGILDAPGLRKMSVAKGKT"
BASE COUNT          426 a          405 c          492 g          314 t
ORIGIN      
        1 acatagccgg ggcggtcatg ggaagggact tctggaagga aggcagaaac atcgaacggc
       61 tgggagtgaa cgagatgtcc gttgaagatc taaggcatta cgttgaaaag ggggacggcg
      121 atggataaga tactggccgg ggcgataggt agcgatattc acaccgccgg aatcctcaac
      181 tttctgggac ttgccaggaa agaagggtac gaatgcgtgt atataggcag cgtgctggga
      241 atagacaagc tgctggacag tataaaagaa gtttcgcccg atatcatagc gataagctac
      301 cggcttggag gagaatcttg cagagaactt ctcagagaat tgcggacggc gctggagaga
      361 gaaggcgtcg aaaagaagat gatcttcggc ggcaccgtcg agacggccgg ggttgcccgt
      421 gaatcgggga tgttcgacag agtcttcgac gggagcgaat cgctggagga agtgacaggc
      481 tatttgcggg gtcgcgacga agcgggaggg accgttgaat acccccaggg attgctcgag
      541 agaattgcct tcaaagagcc tttccccctg attcgtcacc atatcggcct tcaaacgctc
      601 gaagagacgg aactggccgt gaaggaactc gcgcaatcac gtttgctgga tatcatctct
      661 ctcgcgccgg atcaaaactg tcagcaatgg ttcttccatc ccgagaacat ggtccgttcc
      721 gaggatggcg ccggaggtgc gcccttcaga aagagagagg atttcgagag aatgtacgaa
      781 gccagccgga ggggtaacta tccgctagtg cgttcttatt cgggcacgag agagctgttg
      841 aagttctctc tcctcttcaa agagactttg aacaacgcct gggccgccat tccgctcacc
      901 tggtactcac agctcgacgg aagatcggac cgagagcttc tggaggctat aagagaaaac
      961 cagagcgcta tagcctggaa cggcggcagg ggaattcccg tcgagataaa cgaggcccac
     1021 cagtgggctc tcagatactg ccacgacgcc atcgaggtta cgatggcgta tctggccgcc
     1081 atggccgcca gaagactggg agtgaaggtg tacgtggccc agtacatgat gaatacgccc
     1141 ccggggatct cgccttctat ggatatcgcc aagtctctcg cgaaaaagga actgatcgaa
     1201 tcgttgaaga gcgagagttt cattcctgta acgatggtac gtcccggact catgtccttc
     1261 ccggccgatc cccacatgtc gatgggccag ctggtatcct ctctcttcac tgcctcttat
     1321 ctgcaaccgc agatagtcca cgtggtggcc tactgcgaag cgacgaaaag ggccacgccc
     1381 gtggagataa tcgagagcgt gaagatggtg aaacacgcca tgagcgaggc cgcaaaagga
     1441 ctgcccgatt tctcgaagga cgataagata gaacgacaca aagagttcct gaaaaacgaa
     1501 tctgccacta tactcgaggc catggagagt atcaagacct ccagtctgac ctcgcccgag
     1561 accatttacg cagccataaa cgcggggatt ctagacgccc cgggattgag aaaaatgtcg
     1621 gtggcgaagg ggaaaac
//
LOCUS       DAOOLY010000404         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_97744_length_1625_cov_5.440127, whole genome shotgun sequence.
ACCESSION   DAOOLY010000404 DAOOLY010000000
VERSION     DAOOLY010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97744_length_1625_cov_5.440127"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLD15_12620"
     CDS             <1..523
                     /locus_tag="PLD15_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HPB64805.1"
                     /translation="TKSELLDNMAQALGGRAAEDLVFGEITTGASSDLERASAMARSM
                     VTQFGMSERLGPIAWGKEEEEVFLGRELTRMKNYSEEIASEIDSEVKKIVLTSYEKAK
                     RVLSDQRKKLDEAAEFLLQKETISGKELAEILGLDRGNFYKDDFEEANVNVAPGPRTE
                     GGKIMGKPELGNA"
     gene            516..920
                     /locus_tag="PLD15_12625"
     CDS             516..920
                     /locus_tag="PLD15_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64806.1"
                     /translation="MRDLEKLRALTGRMYTFSLTPDDVSLRWSENYDGEILGLLSTSG
                     LLKIVHGCGYSLISEFEVDGLVSVVCETSLRHLSPAKIFEEITVNIDLTVLDGVNIKF
                     SGTVFQNEVLRASFEFVRRFVSLDFLRRSVSA"
     gene            913..>1625
                     /locus_tag="PLD15_12630"
     CDS             913..>1625
                     /locus_tag="PLD15_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB64807.1"
                     /translation="MRKPRDTRSWNEYSLVSALRSKERFVEEIDFTGDVAVALIYPND
                     YRVASSSLSTHVLLRRFNSLPNVRAERFYYVPELERFYSLDSLTPLDEFRIWAFSVHF
                     ELDILNVFDILNSYGIPLKSEDRLPSHPVIVIGGAMTYFNDSLVSRVADVVNRGDLSR
                     NFLERLATLRPSMNRDETASAMTVEPEAPGVFYDDELGQSLFITPESVFKDRFLIEIG
                     RGCARKCRFCVSGYRFGKA"
BASE COUNT          441 a          348 c          442 g          394 t
ORIGIN      
        1 aaccaaatct gagcttctgg acaacatggc ccaggctctc ggtggaaggg ccgccgaaga
       61 cctggtcttc ggcgaaataa ccacaggagc ttccagcgat ctcgaaaggg caagcgccat
      121 ggccaggagc atggtgacgc agttcggcat gagcgagaga ctgggaccga tagcctgggg
      181 caaggaagaa gaagaggtat tcctcggcag agaactgaca aggatgaaga actactccga
      241 ggagatagcg agcgaaatcg attccgaagt gaagaagata gtcctcacca gttacgaaaa
      301 ggccaagagg gtactttctg atcagaggaa aaaactcgat gaagcggccg agttccttct
      361 tcagaaagag actatttcgg gaaaagaact ggccgaaatt ctcggcctcg accgcggcaa
      421 cttctacaaa gacgattttg aagaggcaaa tgtaaatgtc gcccccggtc caagaaccga
      481 aggtggaaaa atcatgggaa agccggagct gggaaatgcg tgatcttgag aaattaagag
      541 ctctcacggg aaggatgtac accttttctc tcactcccga tgatgtttcg ttgcgctggt
      601 cggaaaacta cgatggcgaa atacttggac tgctctctac gagtggactt ttgaagatag
      661 tccacggatg tggctattct ctcatcagcg aatttgaagt cgatggtctg gtgagtgtag
      721 tgtgcgagac ttctctcagg catctgtcgc cagctaagat cttcgaagag ataactgtga
      781 atatagatct aacggtttta gacggtgtaa acatcaaatt ttcaggcacg gtctttcaaa
      841 acgaagtttt aagagcttct tttgagttcg taaggagatt cgtatctctc gattttctca
      901 ggagaagcgt tagtgcgtaa gcctagggat acgagaagct ggaacgaata ctcgctggtg
      961 tcggccttaa gatcgaaaga gcgtttcgtc gaagagatag acttcacagg tgatgtggcc
     1021 gtggctctta tatatcctaa cgattacagg gtagcgagtt ccagtttatc gacacatgta
     1081 ctcttgagaa ggttcaactc cctgccaaac gtgagggccg aacggtttta ctacgttcct
     1141 gaattggaga ggttctattc tctggatagt ttgacaccgc tggacgagtt cagaatctgg
     1201 gccttttcgg tacacttcga actcgatata ctaaacgtct ttgacatttt gaatagttac
     1261 ggtatacccc ttaaaagcga ggatagacta ccctcccatc cagtgatagt tataggcggg
     1321 gccatgacgt atttcaacga ttccctagtt tccagggtag ccgacgtggt caacaggggc
     1381 gatctaagcc ggaattttct ggaaaggctg gccacactca ggccgtccat gaacagagat
     1441 gaaacggcct ctgcaatgac tgtggaaccg gaggctccag gcgtttttta cgacgacgag
     1501 ctggggcaga gtctgttcat cacaccggaa tcggttttca aagatcgttt tctgatagag
     1561 atcggtagag gctgtgcaag aaaatgtaga ttctgtgttt ctggttatcg ctttggtaaa
     1621 gccag
//
LOCUS       DAOOLY010000405         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_98903_length_1613_cov_3.029525, whole genome shotgun sequence.
ACCESSION   DAOOLY010000405 DAOOLY010000000
VERSION     DAOOLY010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98903_length_1613_cov_3.029525"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLD15_12635"
     CDS             <1..664
                     /locus_tag="PLD15_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPB64808.1"
                     /translation="VASILGKPIKELKIITAHIGNGASVAAVKYGRSVDTSMGFTPLE
                     GLVMGTRSGDVDPAIVPFLQEQEGLTAGEVNNLLNKESGMFGLTDRQFVDMRDIEERA
                     AKKDPLCMRAHSIYEYRLAKYIGAYAAAMNGVDAIAFTAGVGENSPFLRENVVNKYLN
                     YLGIELDREKNNCKGCETMISTPQSKVKVMVVPTNEELVIARDTSEIVKKGLKELKLW
                     ED"
     gene            810..>1613
                     /locus_tag="PLD15_12640"
     CDS             810..>1613
                     /locus_tag="PLD15_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPB64809.1"
                     /translation="MAGELADPITITGCNHSGDLFTIGYSSVLVGSKEELREYLVAKP
                     ERLKRVFDSVRQEISACERYRDMDFEDYMKKLIQAKQRWLDEFPPLLFGEKSLYRKGV
                     KLIERGEFAEAEEVLKFYISHYENSPLTWPAMIFYAVSCIYNRKVEVSLKTIVQIMSG
                     ANNELSFLARKMVCYLELLETSYRLITKGPGYGKDFFNHFADLLSDRSSRARGDTVIF
                     EEGNKSNNVVFLLEGNLKFLKTSRSRTSLLFTIDAPDSVGEIHAFTGGSW"
BASE COUNT          477 a          341 c          418 g          377 t
ORIGIN      
        1 agtggcgagc atactcggaa aaccaataaa ggaattaaag ataatcacgg cccacatagg
       61 caacggtgcc tcggtcgcag ccgttaagta tggaagatcg gttgatacat ctatgggttt
      121 cacgccactt gagggtcttg ttatggggac acgctcgggt gatgtagatc cggccatcgt
      181 tcccttcctg caggaacagg aaggccttac ggctggagag gtcaacaatc ttctgaacaa
      241 agagagcggc atgttcggtc ttactgatcg tcagttcgta gatatgaggg acatcgaaga
      301 gagagcggcg aaaaaagatc ccctttgcat gcgtgcgcac agtatttacg agtacagatt
      361 ggccaaatac ataggtgcat acgccgcggc gatgaacggt gttgacgcta tagccttcac
      421 cgccggagtt ggcgaaaaca gccccttcct tagagaaaat gtggtgaata aatatctaaa
      481 ttatttgggg atagaactcg acagagagaa gaacaactgc aaaggttgtg aaaccatgat
      541 ctctactccc caatcgaagg taaaggtaat ggtagtacct accaacgaag agctggtcat
      601 agcaagggat acgagcgaga tagtgaaaaa gggtctgaaa gagctgaagc tctgggaaga
      661 ctgaggaaag gtgctgaagg gaaattagag agaggatttt cgagggagat ctcagactcg
      721 tcgaaagcgg ccgagaacct tatcaattct atatagtctc ttctggtggt cttttactca
      781 aaagtgaagg agtaaatgac aggatatata tggccggtga actcgccgat ccgatcacca
      841 taaccggttg taaccacagt ggagatcttt tcaccatagg ctattcgtcg gtactggtcg
      901 gcagcaagga ggaattaaga gaatacctgg tagccaaacc tgaaagactg aaaagagtct
      961 tcgacagtgt ccgtcaggaa atatctgcct gtgaacgtta cagagatatg gacttcgagg
     1021 attacatgaa gaagctcata caggccaaac aaagatggct cgatgagttc cctccgcttc
     1081 tcttcggtga aaaatctctc tatcgtaaag gtgtaaagct catagagaga ggtgaatttg
     1141 ctgaggccga agaggttctc aaattctaca tctctcatta cgagaactca cctctgacct
     1201 ggccggcgat gatcttttat gcggtaagct gtatctacaa caggaaagta gaagtctccc
     1261 tcaagaccat cgttcagatc atgagtggag caaacaacga actttctttt cttgcaagga
     1321 agatggtttg ttatctcgaa cttctcgaga cctcctatag actgataact aaaggcccgg
     1381 gttacggtaa ggatttcttc aatcatttcg ccgatctcct gtcggacaga tcctccaggg
     1441 ccaggggcga tacagttata tttgaggaag ggaacaagtc caacaacgtg gtctttctgc
     1501 tggaaggaaa tctgaaattt cttaaaacaa gcagaagtag gacatcgcta ctcttcacaa
     1561 tcgacgctcc tgactcggtt ggagagatcc acgcattcac cggaggaagc tgg
//
LOCUS       DAOOLY010000406         1612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_98973_length_1612_cov_3.730250, whole genome shotgun sequence.
ACCESSION   DAOOLY010000406 DAOOLY010000000
VERSION     DAOOLY010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98973_length_1612_cov_3.730250"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /gene="metG"
                     /locus_tag="PLD15_12645"
     CDS             complement(<1..1568)
                     /gene="metG"
                     /locus_tag="PLD15_12645"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPB64810.1"
                     /translation="MKGRYYVTTPIYYINSEPHIGSAYTTIVADVVSRYRRLSDSDVF
                     FLTGTDEHGQKVLSEAVSRKMDTQKYCDILAGKFRQLWKELEISNDGFVRTTDDYHMK
                     AVQFFVNKMKEKGDVYKGKYEGWYCVPDETFWNREDLKEGNLCPECGREVKWVSEENY
                     FFRLSKYNQALIDLFNENPEFVQPDFRRNEMMRILEAGLKDLSITRTSFNWGVPMPED
                     PEHVIYVWVDALINYLSAIGYPDNMEMFQRYWPANVHLIGKEINRFHSIIWPAMLMSA
                     DLPLPGQIYAHGWLTINGQKISKSLGNAVSPREFIKIYGNDALRYYLLRDIQFGRDGD
                     FSEDNLIGRINADLANDLGNLLHRTVVMIDKFNGGLVPRPAERNGTDDELIALAERTF
                     VTYRESMDGLRFTQALEAVWELIRFANKYIDLTEPWKLGKDPAMKGRIDTVLHNLACA
                     LKNISLMIEPVMFHTAREIRYRLGISGEMNLADLSWSKLPGDMRVSHGEPIFPRIDLT
                     THKKVTEMDEEKGK"
BASE COUNT          339 a          431 c          366 g          476 t
ORIGIN      
        1 aattttccct tttcttcgtc catttccgtc accttcttgt gcgtagtaag atctatcctc
       61 gggaagatcg gctccccgtg actgaccctc atatctccag gcaatttgct ccacgaaaga
      121 tcggcgaggt tcatctcgcc tgagattccg agtctgtacc ttatttctct ggccgtgtgg
      181 aacatcacgg gttctatcat cagcgagata tttttcagtg cgcaggccag gttgtgcagt
      241 acggtgtcta tcctcccctt catcgcgggg tcttttccga gcttccacgg ctcggtgagg
      301 tctatgtatt tgttggcgaa tcttataagc tcccataccg cttccagagc ctgtgtgaaa
      361 cgcaggccgt ccatagactc tctgtatgta acgaaagtcc tttcggccag ggcaatcaat
      421 tcgtcatcgg tcccgttcct ctccgctgga cgtggtacca gcccgccgtt gaacttgtcg
      481 atcatcacga cggttctatg aaggagattt cccagatcgt tcgccagatc tgcgttgatc
      541 ctcccgatca ggttatcttc ggagaagtcg ccatctctgc cgaactgtat gtcccttagc
      601 agataatacc tcagagcgtc gttcccgtag atctttatga actctctggg gctgacggcg
      661 tttccgagcg acttggatat cttctgaccg tttatcgtaa gccatccgtg ggcgtagatt
      721 tgaccgggga gcggaagatc tgccgacatc agcatggccg gccagattat cgaatggaac
      781 ctgtttatct ctttgcctat tagatgaacg ttggccggcc agtaacgctg gaacatctcc
      841 atgttgtcgg gatagccgat agcgctgaga tagtttatca gggcgtccac ccatacgtat
      901 attacgtgct cgggatcttc gggcatggga acgccccagt taaaagaggt cctggttatg
      961 cttagatcct tgaggccagc ttccagaatc ctcatcattt cattccttct gaaatcgggt
     1021 tgaacgaact ccgggttttc gttgaagaga tctatgaggg cctggttgta tttcgaaagc
     1081 ctgaagaagt aattctcttc gcttacccat tttacttctc ttccacactc ggggcagagg
     1141 tttccttcct taagatcttc tctgttccag aaagtctcgt cgggaacaca gtaccatccc
     1201 tcgtacttgc ctttgtaaac gtctcccttt tccttcattt tgtttacgaa gaactgaacg
     1261 gccttcatat ggtagtcatc agtagtcctc acgaaaccgt cgttacttat ctcaagctct
     1321 ttccacagct gcctgaactt tccggccaaa atatcacagt atttctgtgt gtccatcttc
     1381 ctcgatacgg cctcgctcag gaccttctga ccatgttcat cggtccccgt cagaaagaaa
     1441 acatcgctgt ctgaaagcct cctgtatctt gatacgacgt cggcgactat ggtggtgtac
     1501 gccgaaccga tgtgcggttc gctgtttata taataaatag gcgtggttac atagtatctc
     1561 cctttcacag gacacctcct cgaatattat agcactaatc tatttgaatg ta
//
LOCUS       DAOOLY010000407         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_99053_length_1611_cov_4.904242, whole genome shotgun sequence.
ACCESSION   DAOOLY010000407 DAOOLY010000000
VERSION     DAOOLY010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99053_length_1611_cov_4.904242"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLD15_12650"
     CDS             complement(<1..735)
                     /locus_tag="PLD15_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64811.1"
                     /translation="MLKLSLFGKIMVYILLAVYCLVILVPFFMMILNSLKSMRDIFMK
                     PFSFPTKVLYDNYTKAWNQAGIGTGYKNSAFVAGVSVIGILIVSSMFAYAISKYEFKG
                     RRFLFVYSMLGLAFPARLAIIPIFILLRNLTLTNSLFGLILIYTSVNIPFSVFLLKNF
                     IDGVPNELSEAARIDGATPMQIYWKVVLPLVKPALSIVAIVSFVNVWNDFFFPLIFIN
                     DKSKATITLAVSIFFGEYSNQWPLLFS"
     gene            complement(739..>1611)
                     /locus_tag="PLD15_12655"
     CDS             complement(739..>1611)
                     /locus_tag="PLD15_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB64812.1"
                     /translation="FIIPAMLLYTVFIIYPLFDSLILSFFSWPGVGSRTFVGFKNFQT
                     LFTGSYAKELFNAFLHNVYFFLLLILLELGLGFLIALMLASKIKGAGVFRVITYIPNM
                     IPLVLVGFMWGLFLNPQVGLVNQLLKLVGLGGLAQPWLGLENTALTTIILVNVWRNLG
                     FYVLVILAAILDISPDLIEAAYIDGANNWKITWRIIFPLTFSTFRTLAILLFIWSFNI
                     FDTVYALTGVQAGPYRSTDVLGTLFYRTAFGGLGSGAVDMGLGASVTVFIFMIVMPVS
                     ILYVYLVEKRQRSA"
BASE COUNT          461 a          369 c          424 g          357 t
ORIGIN      
        1 cgagaacaaa agcggccact ggttcgaata ttctccgaag aagatcgaaa cggcgagcgt
       61 tatggtggcc ttcgatttgt cgtttatgaa tatgagcggg aagaagaagt cgttccagac
      121 attgacgaaa ctcactatcg ccacgatcga aagagccggt ttcacaaggg gcaacacgac
      181 tttccagtaa atctgcatgg gtgtggcccc gtctatcctt gcggcctccg acagttcgtt
      241 cggaacgccg tctatgaagt tcttgagcag gaagaccgaa aagggtatgt tcacggacgt
      301 atatatcaga atcagaccga aaagcgaatt agtgagcgta aggtttctca gaagtatgaa
      361 gatcggtatt atagccagtc tcgcggggaa ggcaagcccg agcatcgagt agacgaaaag
      421 gaacctgcgc cccttgaatt cgtacttgga gatggcgtat gcgaacatgg aggagactat
      481 cagtattcct attacggaca cccccgccac gaaggcgctg tttttgtagc ccgtacctat
      541 gccggcctgg ttccaggctt tggtgtagtt atcgtacagt actttcgtgg ggaaggagaa
      601 gggcttcatg aatatatccc tcatggattt cagggagttc aggatcatca tgaaaaaggg
      661 gacgagaatg accagacagt aaacggccag aagtatgtac accattattt tcccgaaaag
      721 actcaattta agcatttctc aagcactcct ttgtctcttc tccacgaggt aaacgtaaag
      781 gattgaaacg ggcatgacta tcatgaatat gaaaactgtg accgacgcgc ccagacccat
      841 atctaccgca ccgcttccca gcccgccgaa agccgttctg tagaagagtg ttccgagaac
      901 gtcggtggat ctgtagggac cggcttgaac acccgtcagg gcgtaaaccg tgtcgaatat
      961 attgaaactc catatgaaaa ggagaatcgc cagcgttctg aacgttgaaa aagtaagggg
     1021 gaatattatc cgccaggtta ttttccagtt gttggctcca tctatgtagg cggcctctat
     1081 caaatccggc gagatatcca gtatggcggc caggatgacc aggacgtaaa agccgagatt
     1141 acgccagacg ttcacgagga ttatagtcgt aagcgcggtg ttctccagac ctagccacgg
     1201 ttgtgccagt ccaccgagtc ccaccagttt gaggagctgg ttaaccagac ccacctgggg
     1261 attcaggaag agtccccaca tgaaaccgac cagaaccagg ggtatcatgt ttggaatgta
     1321 cgtgatgact ctgaacacgc ccgctccctt tattttgctg gccagcatca gggctattag
     1381 aaagccaagt ccaagttcaa gcaggataag gagcagaaag aaataaacgt tgtgcagaaa
     1441 agcgttgaaa agctctttgg cgtaggaacc ggtgaaaagc gtctgaaagt ttttaaagcc
     1501 cacaaaggtt ctcgatccaa ctcccggcca gctgaagaaa ctgagaatga gactgtcaaa
     1561 gagcggatag atgatgaaga ccgtatagag aagcatcgct ggaattatga a
//
LOCUS       DAOOLY010000408         1599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_100182_length_1599_cov_3.778497, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000408 DAOOLY010000000
VERSION     DAOOLY010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100182_length_1599_cov_3.778497"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1232
                     /locus_tag="PLD15_12660"
     CDS             153..1232
                     /locus_tag="PLD15_12660"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB64813.1"
                     /translation="MFTLIAWLTGLLIFGGLKVLEASHSKDGGTGRKVSLIIPARNEE
                     KNIGKLLESISKQKTVPHEVIVVDDNSSDGTAEIASDYNVKLVRLESDPPTGWIGKSW
                     ACWNGYLNSAGETLVFLDADVELSPEALGTIIDLQELKGGLVSVQPYHRMERFYESFS
                     LVFNLIVVASLGNFMAGERNAPGAFGPCMVCSRSDYSDVGGHEMIRSNVLDDIELGRA
                     FRKSGFLVSNTFGGELISFRMYPGGIGEVLKGWGKNFAKGAGSTPPLTLLLMILWITG
                     GFSTFLRLPIALGFQIQFIAFIIMYLAYAIQIYLYSRKIGSFSIGHALIFPVYFLFFF
                     YTFIYSGLRTLLFRRVSWKGRDIDV"
     gene            1225..>1599
                     /locus_tag="PLD15_12665"
     CDS             1225..>1599
                     /locus_tag="PLD15_12665"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPB64814.1"
                     /translation="MSEVIFLLLFLLEFFMGSMMFSYWLGKIKGKDIRQTRDGNPGGS
                     NLGRIFGWKWGMAGIALDFLKGYVPLVFIVESGILKDWRLAVVSVAPVLGHAFSPFLK
                     FSGGKAVAVTFGIWSALTKWEVP"
BASE COUNT          342 a          400 c          441 g          416 t
ORIGIN      
        1 taatacacca cgcactgcaa ttatttagaa tcaggcctct acattacgct caacacaatg
       61 ttttgctccc gacggccact tcgcctgaga agcagaaatg ctagaattgt tctaaacagt
      121 tgtagcggga gggaaggttt ctgttcatat tgttgttcac cttgatagcc tggttaacgg
      181 gcttactgat tttcggcggg ttaaaagttc tggaagcctc gcattcgaag gatggtggca
      241 ctggaaggaa agtctctcta ataatcccgg ccaggaacga ggagaagaat atcgggaagc
      301 tactggaatc gatttcgaag cagaagaccg ttccgcacga agtgatcgta gttgacgaca
      361 attcgtcgga cggaactgcg gagatcgctt cggactacaa tgtcaaactt gtcaggctcg
      421 aatccgaccc gccgacggga tggataggca agtcctgggc ctgctggaac ggctacctca
      481 attccgccgg agagaccctg gtcttcctgg atgcggacgt agaactctct cctgaagctc
      541 tcggcaccat catcgacctt caagagctca aaggcggcct ggtttccgtc cagccttacc
      601 acagaatgga acgcttctac gagagcttct cgctggtatt caatctcata gtggtcgctt
      661 cgcttgggaa tttcatggcc ggcgaaagga acgcgccggg agctttcggc ccctgcatgg
      721 tctgctcgcg aagcgattat tccgacgtgg gcggtcacga aatgataaga agcaacgttc
      781 ttgacgatat cgagctggga agggcctttc ggaaaagtgg attccttgtg agcaacacct
      841 ttggaggcga gctgatcagt ttcaggatgt acccgggtgg gatcggggaa gttctgaagg
      901 gctggggaaa gaacttcgcg aagggcgccg gctctacacc gccgctcacc ctcttgctga
      961 tgatcctgtg gatcaccggg ggcttttcta cctttctgag actgcccatc gccttaggtt
     1021 tccagatcca gttcatcgcg ttcatcatca tgtatctggc ttatgccatc cagatctacc
     1081 tgtactcaag gaagatcggc tccttctcga taggccacgc ccttatcttt ccggtctatt
     1141 ttctcttttt cttctatacc ttcatctact caggtttgag aactctgctt ttcagacgtg
     1201 tcagctggaa gggaagggat atagatgtct gaagttatct ttcttcttct ttttctgctg
     1261 gagttcttca tgggttcgat gatgttctcg tactggctgg ggaagatcaa gggaaaggac
     1321 ataaggcaaa ccagggacgg caaccccggc ggctctaatc tgggaaggat cttcggctgg
     1381 aagtggggta tggccggcat agctctggat tttctcaagg gctacgttcc gctggttttc
     1441 atcgtagaat cggggatttt gaaagactgg cgactggcgg tcgtttcggt cgcacccgtt
     1501 ctcggccacg ccttctcgcc cttcctgaag ttttcggggg ggaaggccgt cgccgttacc
     1561 ttcggcatct ggtcggcttt gaccaagtgg gaggttccc
//
LOCUS       DAOOLY010000409         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_101681_length_1583_cov_3.498037, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000409 DAOOLY010000000
VERSION     DAOOLY010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101681_length_1583_cov_3.498037"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLD15_12670"
     CDS             complement(<1..197)
                     /locus_tag="PLD15_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64815.1"
                     /translation="MIEAFVNDTLRNATPIVFAACGGLVTKLSGWLNIGLEGMILFSA
                     FSGVVVSPATSSVTAGTIMAIA"
     gene            complement(190..1221)
                     /locus_tag="PLD15_12675"
     CDS             complement(190..1221)
                     /locus_tag="PLD15_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB64816.1"
                     /translation="MKGRAFTVARALFTLAIALAVGFVVIVLTEEEPLEATSAFFLSV
                     FTNTFYFGNLLSASIPLIFTGLAASIAFSSSSFNLGLEGQVYLGALTGTYIGVRMGGT
                     SPLIVVPVVLLSGFLSGALVAGISGLLKGFRGVNELISSLLLSNGIVLVVDYIIEGPF
                     NDRPSGLAASTSLGKEVILPRILEPSSLHSGLFLALITAVLLWAFMFRSRPGYDFRVT
                     GKSSIFAYYGGINVKAVYFWSMFLSGGLAAVGGTVDVLGVHGRVLRGFSGGYGWNGIA
                     VALIARNHPLGVIPAALFFAYLESGAQIASFEAGLTPEISRIVQSVVFYLVTAEAVLS
                     FMKGGRKVD"
     gene            complement(1218..>1583)
                     /locus_tag="PLD15_12680"
     CDS             complement(1218..>1583)
                     /locus_tag="PLD15_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPB64817.1"
                     /translation="STYGVEYSSLDDQAGTLSGGNLQKAILAREISGNPRLILLSEPT
                     RGLDVKHAGMVHRTLLSLKQNLSIVLFTGDLDEAISIADRILIMYDGEVVIDLPNAGK
                     IGRSMLNRYLQGAAGVEST"
BASE COUNT          410 a          444 c          403 g          326 t
ORIGIN      
        1 gcgatcgcca tgatggtgcc ggctgttaca ctggaagtcg cgggtgatac gaccacccca
       61 gagaaggccg aaaaaagtat cattccttcg agaccgatgt ttagccagcc cgaaagcttt
      121 gtcacaagac caccacaggc ggcgaaaaca atcggggtcg cgtttctcaa cgtatcgttc
      181 acaaaggcct caatcaacct tccgtccccc cttcataaaa gagagtacgg cttcggcagt
      241 taccagatag aacaccaccg actggacaat cctggatatc tcgggtgtaa gaccggcttc
      301 gaacgaggct atctgcgcgc cgctttcgag ataggcaaag aaaagtgccg ccggtatgac
      361 tcccagagga tggttccttg caatgagagc gactgcgatt ccgttccagc cgtaaccccc
      421 ggagaaaccc ctcaggactc tcccgtgaac gccaagcaca tctactgtac cgcctacggc
      481 ggccagtcct ccgctgagaa acatagacca gaaataaacg gccttcacgt ttataccgcc
      541 gtagtaggcg aagatggaac tcttgcccgt cacgcgaaaa tcgtatcccg gtctgctcct
      601 gaacataaag gcccagagca gaaccgccgt gatcagggcc aggaagagtc ccgaatgaag
      661 actcgaaggt tccagaatcc tgggtaagat gacttccttt ccaagactcg tggaagccgc
      721 caaccccgag ggtctatcgt tgaagggacc ttctattatg taatcgacaa ccagtactat
      781 cccgttgctc agcaggagcg aggagatcaa ctcgttcaca cctctgaagc ctttcaacag
      841 cccggaaatt ccggcaacta gggctcctga gagaaagccg gagagcaaaa ccacaggtac
      901 cactataagt ggagaggtac ctcccatacg aacacctata tatgttcccg tcagcgctcc
      961 gagatagacc tggccttcaa gaccgagatt gaaactcgag cttgagaagg caatggacgc
     1021 cgcaagtcca gtgaagatca gtgggatcga ggctgagagt aagttcccga agtagaaggt
     1081 gtttgtgaaa acggaaagaa agaaggcgga ggttgcctcg agcggttcct cctccgtcaa
     1141 gacaatcact acaaatccaa cggccagggc gattgcgaga gtgaagagcg cccttgcaac
     1201 tgtaaacgct ctccctttca cgtactctcc acccctgccg ctccctggag ataacggttc
     1261 aacatcgatc tcccgatttt tcccgcgttc ggcagatcta taactacctc gccgtcgtac
     1321 atgattaata tcctgtcggc tattgagata gcctcgtcca ggtctcctgt gaagagaact
     1381 atggagaggt tctgcttgag agagagaagc gttcggtgta ccatcccggc gtgtttgaca
     1441 tccagtcctc tggtcggctc gctcaagagt atcaatcggg gattaccgga gatctctcta
     1501 gccagtatcg ccttctgtaa gttgccacca gagagtgttc ctgcctggtc gtccagagaa
     1561 gagtattcga ccccgtaggt cga
//
LOCUS       DAOOLY010000410         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_102858_length_1571_cov_3.117414, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000410 DAOOLY010000000
VERSION     DAOOLY010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102858_length_1571_cov_3.117414"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLD15_12685"
     CDS             <1..968
                     /locus_tag="PLD15_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64818.1"
                     /translation="DQGEKILTEAALLYHFANHVKPRIVVSDRDVNNRMRSIYENYPL
                     LGNVAEIVEVEFLDLIRGEVDWEIFSTISSCFITTENEGESSFVARKLRQMFEVKVDS
                     DLSGHGRKVSSVPAIVAVIPMLSRFFDIVPETRQKLETVNIDISNPGDVLNTDNLFST
                     NEEIEKIAMYFHWEYNKRKDKTLDKVWNELEDSEKDSNRYAARHLPIKLRFLGVRIAD
                     ASTDCPTFDQNTVTEDQKRTMHIMEHRRWKAYRELNGFVYGQKWFKEDGKSNKEVMNM
                     LMINNSIKPWHELEANIKEIDSIFVNAGDAVAELNKKIVVDYQKS"
     gene            1015..1413
                     /locus_tag="PLD15_12690"
     CDS             1015..1413
                     /locus_tag="PLD15_12690"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HPB64819.1"
                     /translation="MPERPLRIFISYGHDQYAELAERIKNDLNEKHPGVWFDRDRIKP
                     GKDFELYMENGLEWLTEDKSRASFLYLMTPHSVRRPNGFCLNELTSAMVKELSIFPVM
                     VQMSEPPLSICRIQWLDMQECFPPDEKADS"
BASE COUNT          509 a          310 c          394 g          358 t
ORIGIN      
        1 cagatcaggg tgagaagatc ctgacggaag cggctctgtt ataccatttt gcgaatcacg
       61 taaaaccgag aatcgttgta tcggatagag atgtgaataa caggatgagg tcgatctacg
      121 agaactaccc attgctggga aacgttgcgg agatagtaga agtagagttt cttgacctta
      181 taaggggaga agtcgactgg gaaatcttct caacaatttc ctcatgcttc ataacgactg
      241 aaaacgaggg agagagttct ttcgtagcca gaaagctcag gcagatgttt gaggtaaaag
      301 tagacagtga tttaagtggc catggtagaa aagtgtcaag tgtaccagca attgtggcag
      361 taatacctat gctttctagg ttctttgata tagttccaga aaccaggcag aagctggaga
      421 ccgtgaacat agacatctca aacccaggag atgtgctcaa tacagataac ctgttctcta
      481 ctaacgaaga aatagagaag atagccatgt actttcactg ggaatacaac aagagaaaag
      541 ataagacact cgacaaggtt tggaacgaac tggaggattc ggaaaaagac tctaaccgtt
      601 acgctgccag gcacttaccg atcaaactga ggtttctggg cgtaagaata gccgatgctt
      661 caactgactg ccctaccttc gatcagaata cagtcaccga agatcaaaaa aggacaatgc
      721 acatcatgga acatagaaga tggaaggcat atagagagct taacggattc gtttatggcc
      781 aaaagtggtt caaagaagat gggaaatcga acaaggaagt catgaacatg ttaatgataa
      841 acaacagcat aaaaccatgg catgagctgg aagccaatat caaggagata gactcgattt
      901 tcgttaatgc tggcgacgct gtagccgaac tgaacaagaa gatcgtagta gattatcaga
      961 agagctaatc gggagacata ggatcgctaa ctgggataaa ccgggaggtt tacaatgcca
     1021 gaaagaccac taaggatatt cataagctac ggtcatgacc agtatgccga gctcgcagag
     1081 agaataaaga acgatctcaa tgagaaacat cctggtgtct ggttcgacag ggacagaatt
     1141 aagccgggca aagactttga gctttacatg gagaatggtc tcgagtggct gacagaggat
     1201 aaatccagag cgagcttcct ctatcttatg actccacatt ctgtgagaag gccgaacggt
     1261 ttctgtctga acgaactcac ttccgccatg gtgaaggagc tctccatttt ccccgtgatg
     1321 gttcagatga gcgaacctcc cctctccatc tgcagaatac agtggctcga tatgcaggag
     1381 tgttttcctc cggatgaaaa agcggattct tagtccaaaa gtttcaagaa gcttctccag
     1441 gctctcgaag acgatagtta ggattttgaa ggttctacaa agagccttct ggtaaagcta
     1501 gatccgagtg acttcggacc ggatatggtg agacacctga aagacttcac cggaagagaa
     1561 ttgctcaagg a
//
LOCUS       DAOOLY010000411         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_104149_length_1558_cov_4.846307, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000411 DAOOLY010000000
VERSION     DAOOLY010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104149_length_1558_cov_4.846307"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1026)
                     /locus_tag="PLD15_12695"
     CDS             complement(<1..>1026)
                     /locus_tag="PLD15_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB64820.1"
                     /translation="SSRSLDVVPFDTYRSLLNTFGGASWKNGGQAVFYEFEVPSDGYY
                     SIAFKYIQDSKSKMRVFRKITIDGAVPYKELESYPFDSSTDWKIETLESNGESLEFYL
                     SAGRHTLGISVNVSVYESVLMTLKTMIAHINDFALQIRYLTGGGKIDRNVEWEIGDYF
                     PGIDETLAAMREALKGEYSTASRINGNEDSQELTSFRTAIMLLERLMKDPDEIPKKLD
                     LLVGSVGSIVSELSVALNGFQNMPLTLDRVYIYAGESAPSFKRASVFASLWEGIKRFV
                     DSFRPARYEAGGSEGSVVLEVWINRARQYSDIIQQLVDDDFTPETGIVVDLSIIKDEQ
                     KLILANAA"
     assembly_gap    1027..1036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1037..1513)
                     /locus_tag="PLD15_12700"
     CDS             complement(<1037..1513)
                     /locus_tag="PLD15_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB64821.1"
                     /translation="MNIPLNAGGYYAIRLGYTVTGSSILPPEVSVEINGAHQFLEAQR
                     VALVQHWKYETLNFPLNRYGDEVLPSQTMLSTRLDYTLRDSSSLFREPLLFSLEEGEN
                     TVKVTLLSGEITLHSLEVLSLEAIPAYARPDADRPKGDFITIVEAEHPSYKSDTTIN"
BASE COUNT          307 a          418 c          441 g          382 t
ORIGIN      
        1 gccgcgttgg ccaggatcag cttctgttca tctttgatga tcgatagatc caccactatc
       61 cccgtttcgg gggtgaaatc gtcgtccacg agctgctgga ttatgtccga gtactgtctg
      121 gctctgttta tccacacttc cagaacgacc gatccctcgc tccccccggc ttcgtaccgg
      181 gccggtctga aggagtcgac gaacctcttg atcccctccc agagagaggc gaagacggag
      241 gccctcttga aggagggagc ggattcaccg gcgtagatat atacgcggtc gagagtgagc
      301 ggcatattct ggaagccgtt cagggcgacg gagagttccg agacgatcga cccgacgctt
      361 ccgaccagca aatcgagctt tttcggtatc tcgtcggggt ctttcataag cctctccaga
      421 agcattatgg ccgttctgaa agaggtcaat tcctgtgaat cttcgttgcc gtttatccgg
      481 gaggccgttg agtattcccc cttgagcgcc tctctcatgg cggccagagt ttcatctatc
      541 ccggggaagt agtccccgat ctcccactcc acgttcctgt cgatcttgcc cccgccagtg
      601 aggtatctta tctgcagggc gaagtcgttt atatgggcta tcatagtttt caaagtcata
      661 aggacgcttt cgtaaacgct cacattgacg gaaattccga gcgtgtgcct gcccgcgctg
      721 agatagaact ccaggctctc cccgttgctt tcgagagttt ctatcttcca gtcggtcgag
      781 gaatcgaagg ggtagctttc gagctcttta tatggcacgg caccgtctat cgttatcttt
      841 ctgaagactc tcatcttcga tttcgagtcc tgaatgtatt tgaaggctat actgtaatat
      901 ccgtcggagg gtacctcgaa ttcgtagaag acggcctgtc cgccgttctt ccacgaggcc
      961 ccgccaaagg tgttgagaag cgagcggtaa gtgtcgaagg gaacgacatc gagactcctg
     1021 ctcgagnnnn nnnnnngtta atggtggtgt cgctcttgta agaagggtgt tcggcctcca
     1081 ctatcgtgat gaaatcccct ttcggccggt ccgcatcggg tctcgcgtaa gcggggatcg
     1141 cctcgagcga tagcacttcc agcgagtgca gggttatctc tccggagagg agtgtcactt
     1201 tcaccgtgtt ttcgccctct tcgagagaga agagcagcgg ttccctgaaa agactcgagg
     1261 agtcgcgcag cgtgtaatcg agccgggtcg aaagcatcgt ctgagagggc aggacttcgt
     1321 ccccgtacct gttcagcggg aaatttagcg tctcgtattt ccagtgctga acgagcgcca
     1381 ctctctgcgc ttcgaggaac tggtgggcac cgtttatctc gacggagacc tccggcggga
     1441 ggatcgaaga gcctgtaacc gtgtagccga gtctgatggc gtagtagccg cccgcgttca
     1501 gaggtatgtt caactcgagg ccgtcgtttc tccggaggtt ttcagaaagg ggcggcga
//
LOCUS       DAOOLY010000412         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_104598_length_1554_cov_3.619079, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000412 DAOOLY010000000
VERSION     DAOOLY010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104598_length_1554_cov_3.619079"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLD15_12705"
     CDS             complement(<1..295)
                     /locus_tag="PLD15_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPB64822.1"
                     /translation="MASKASKEIMKEINEKLLLRAIYESDGIDRASLAKMTGLSPATV
                     TKVVGDLIEIGLVEETGVADSTGGRKPILLNVRQGKSCVLGLKIGVGYLDYSLT"
     gene            complement(352..>1554)
                     /locus_tag="PLD15_12710"
     CDS             complement(352..>1554)
                     /locus_tag="PLD15_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPB64823.1"
                     /translation="SMALTTSLLGVTTIKFMNFSSADANAMYLEEMRKIFEEQNPDIK
                     VQIETVGFGDYFTKLMTVVAGGNAPDAFELNYENFYTYAKKGVLLNLNDLVATSGFDT
                     SVLNEKALYAFSEGGVQYGLPFSFSNVVLIYNKDLFDKAGVEYPTDDWTWADELEAAK
                     KIRALDAMAFGVYQPVQFWEFYKMVQQNGGSILNEDRTAFTLNTPQNVETLQFMVDRI
                     LKYNVMPDDAQMAGMGDWDLFEVERIGMIVTGTWAFSTFKNSCDFEWDIAVEPGNTGK
                     ATHFFANGLAISKDSKNVDAAFRWISFLSGDLDVAKIRIEAGWELPPVTYPEIIELYK
                     QTTPPASKEVVFRSLDYLVTPPVIEQFNEMADIVNRHLEAARYGDKTPQKALDDAQAE
                     LEKAIKLN"
BASE COUNT          352 a          396 c          357 g          449 t
ORIGIN      
        1 cggtcagcga ataatcaaga tatccaacgc ctattttgag acccagtaca cacgattttc
       61 cctgtcgtac attcaggaga ataggttttc tccctcccgt agagtcggca acaccggttt
      121 cctctaccaa tccaatctct attaagtctc ccactacttt ggttaccgtg gccgggctca
      181 aacctgtcat ttttgccagg ctcgccctgt cgataccgtc gctttcgtat atagctctca
      241 ggagcagttt ttcgtttatc tctttcatta tctctttgct agctttactc gccacggcaa
      301 aaacccccat agagagggcc ggggaaaccc cggccctgat tcaatcagtt attagttcaa
      361 cttgatagct ttttcgagtt cggcctgggc atcgtcgaga gctttttgcg gagtcttgtc
      421 tccatagcgg gcagcctcaa gatgtctgtt aactatgtcg gccatttcgt tgaactgttc
      481 tatcactggc ggtgtcacca gataatccag cgatctgaag acgacttctt tgctggctgg
      541 aggtgtggtc tgcttgtata gctcaatgat ctcggggtag gttacaggag gtagctccca
      601 gccagcttcg attctgatct tagcgacatc gaggtctcct gaaaggaagc tgatccacct
      661 gaaggccgca tctacgttct tagaatcttt gctaattgca agaccgtttg cgaaaaagtg
      721 ggttgctttg cctgtgttgc caggctcaac cgctatatcc cattcaaaat cgcaagagtt
      781 tttgaatgtc gagaatgccc atgtgccggt aacgatcatt ccgattcttt ctacttcgaa
      841 gaggtcccag tcgcccattc cggccatctg agcatcatcc ggcataacat tgtacttgag
      901 aattctatcc accatgaact ggagtgtttc aacgttctgc ggggtgttca gggtgaaggc
      961 cgtcctgtcc tcgttgagaa tactgcctcc gttttgctga accatcttgt agaactccca
     1021 gaactgaacg ggctgataaa ccccaaaggc catcgcatct agcgccctga ttttcttggc
     1081 agcctcgagt tcgtcggccc acgtccagtc gtcggttgga tattctacgc cggctttgtc
     1141 gaagagatct ttgttgtaaa tcaaaaccac gtttgaaaaa ctgaacggca gtccatactg
     1201 tactccgcct tcagaaaagg cgtacagagc cttttcgttg aggacactgg tgtcgaaacc
     1261 ggaagttgct accaggtcgt tgaggttcag caatacacct ttcttggcgt aagtgtagaa
     1321 gttctcataa ttcagttcaa atgcatcggg ggcgtttcca ccggctacta cggtcattag
     1381 ttttgtgaaa tagtcgccaa aaccaacggt ttcaatctgc accttaatat ccgggttctg
     1441 ctcttcgaag atctttctca tctcttcaag atacatcgca tttgcatctg ctgaagagaa
     1501 gttcatgaac ttgatggttg tcacaccgag tagagaggtt gtcagtgcca ttga
//
LOCUS       DAOOLY010000413         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_105389_length_1546_cov_4.959088, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000413 DAOOLY010000000
VERSION     DAOOLY010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105389_length_1546_cov_4.959088"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLD15_12715"
     CDS             <1..922
                     /locus_tag="PLD15_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64824.1"
                     /translation="FSFGVGTGVVAELLDRLVQENAGRVSYIVEGENIESKVTELYRS
                     IETPALENVVISMEGIETAKILPTGPHSLFSGYALRLSGLFFEEGDLKVTVEGTRGGE
                     RHTYEYYFRIGKIAENEFVSRIWAQKRIASLANIYRYDSSLSEKAREEVAQEIIALSK
                     KFNIINEFTSFLIAPERPLADNAMVDRGGLGGVPQTSGSVMAAKSVAEMEQDQFVGGG
                     ASDYRFVGSIGFYLKDGVWIEDNPEVEKLVPVKVKAFSQAYFDLFNVDDWLKDVLSLG
                     EKVKFIYEGTLFETGDAGIEDEKELEEFLK"
     gene            complement(976..>1546)
                     /locus_tag="PLD15_12720"
     CDS             complement(976..>1546)
                     /locus_tag="PLD15_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPB64825.1"
                     /translation="APGGWMTSYTDPNGIYSTGWTPSGNNYMYMQGTSMATPHVTGLV
                     ALLMGAGLTDPDEIRQTLQDTATDLGTGGRDDYYGYGLVNAQAALDSITTPQEFKVFL
                     RNPATGVDIANTTISDSGSYGFSNVTLAQVKVWAWRDMDESGTINTGDLLGYYNYNGG
                     KPNVNNATTYNLSTGDNWIDFKFAPIVGN"
BASE COUNT          348 a          387 c          446 g          365 t
ORIGIN      
        1 cttctcgttt ggcgtaggta ctggagtcgt tgccgaactt ctggaccggc tcgttcagga
       61 aaatgccgga agggtcagtt acatagtgga gggcgaaaat atcgagagta aagtgaccga
      121 actttacagg tcgatcgaaa cccccgcgct cgagaacgtc gtgatctcta tggaagggat
      181 agaaaccgcg aaaatactac cgactggccc ccattcgctc ttctccggtt acgcgctgag
      241 gttatccggc ctcttcttcg aagagggtga ccttaaagtt acggtagaag gtacgcgcgg
      301 tggcgagaga catacatacg agtattattt ccggatcggc aagatcgccg aaaatgaatt
      361 cgtctcaaga atatgggcac agaagagaat cgcctcgctg gccaatatct atcgctacga
      421 tagcagtctc agcgagaagg ccagggagga agtcgcacag gagataatcg cactttcgaa
      481 gaagttcaat ataataaacg aattcacttc cttcctcata gctcccgaaa gacctctggc
      541 agacaatgca atggttgaca ggggaggatt gggaggcgtc ccgcagactt cgggatcggt
      601 gatggcagcc aaatcggtcg ccgaaatgga acaggaccag ttcgtcggag gcggtgcgtc
      661 cgattacagg tttgtcggaa gcatcggatt ctacctcaag gatggagtgt ggatagagga
      721 caatccagag gtcgaaaaac tcgtaccggt aaaggtaaaa gcgttcagcc aggcttattt
      781 cgacctgttc aacgtcgatg actggttgaa agacgtcctc tccctcggtg agaaggtcaa
      841 gttcatatac gaggggactc tcttcgagac gggcgatgct ggtatagagg atgaaaaaga
      901 gctggaagag tttttgaagt gatcgccaaa aacaaggggg gagctcgcgg gagctccccc
      961 cttttgtcgt ttttatcagt ttccgacgat cggagcgaac ttgaagtcga tccagttgtc
     1021 gcccgtgctg aggttgtacg ttgttgcgtt gtttacgttc ggctttccac cgttgtagtt
     1081 gtagtaaccg agcaggtctc ccgtgttgat cgtaccgctc tcatccatgt ctctccaggc
     1141 ccagaccttc acctgagcca gcgttacgtt gctgaagccg taagagccgg aatcggatat
     1201 cgtcgtgttg gctatgtcta cgcctgtcgc cgggttcctc aggaagacct tgaactcctg
     1261 cggcgtggtt atcgaatcga gagccgcctg cgcgttgacc agtccgtaac cgtaataatc
     1321 gtctcttcct cccgttccca gatccgtcgc ggtgtcctgg agtgtctggc gtatctcgtc
     1381 cgggtcggtg aggccggccc ccatcagtag cgcgacgagc ccggtcacgt gtggcgtggc
     1441 catcgatgtg ccctgcatgt acatgtagtt gttgcccgaa ggcgtccagc cagtgctgta
     1501 tattccgttc gggtctgtgt acgatgtcat ccagcctccc ggtgcc
//
LOCUS       DAOOLY010000414         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_105592_length_1544_cov_4.398254, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000414 DAOOLY010000000
VERSION     DAOOLY010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105592_length_1544_cov_4.398254"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..>1544)
                     /locus_tag="PLD15_12725"
     CDS             complement(69..>1544)
                     /locus_tag="PLD15_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPB64826.1"
                     /translation="DPSRLEKVDDLISQSSVPLQTALNLLVTSFEERVHGIMTSLRVL
                     AGSPGVISGEWSRMKPLLSSFNQGYEGLAMYVEPDGGYYTVQRDWTGLNLSDRGYFSA
                     LLMGGEVLGYQVLSRSTGKRSVVFATPVRKEDNISGFVGISAFFDEWNFELISDFKQL
                     PEVHFYAFDEQNTLALSDDTSLLLGSLEVPVPGITERLATLDRDSSDFLFTGNDVLHL
                     AAYRRSLKTGWTFVIARELLSTMRDPRPELLLSKVQKEIQEHFNRLDTSLSKGAEDMR
                     KILNDDEEVRSLLKRLYEANSEVVDVSYIDGDCVLRLIHPRDYSYVEGEWIGDQEQLI
                     KLHETGRPVLSEGFLAVEGFQAVDLEWPVFAEDGTLAGSLSFLIKPETFLAPVIIPNN
                     LEPYEFWIMQPDGTIFYDQDSLEVGRNLFEDELYESFDTLVELGYSISKNESGQGYYT
                     FYSRGLSSTVKKEVWWSSIGLHGTQWRLILTKIINPTSVSQ"
BASE COUNT          376 a          400 c          372 g          396 t
ORIGIN      
        1 ggcgatgttt tattcgccaa tcggttttcc agcggagaag ttgttctgag ttctagattt
       61 gcaacacgct attgagatac cgaggtcggg ttgatgattt tggtcaaaat gagcctccac
      121 tgcgttccgt gaagtcctat ggaactccac cagacttctt ttttgacagt gctggataaa
      181 cctctcgagt agaaagtgta ataaccttga ccactctcgt ttttagagat cgaatatccc
      241 aactcgacaa gtgtatcgaa agattcgtat agttcatcct cgaagaggtt tcttccaact
      301 tccaggctgt cctggtcgta gaatatcgtg ccatcgggct gcattatcca gaattcgtag
      361 ggttccaaat tgttgggaat tattaccggg gccaggaagg tttcgggctt aatcaggaag
      421 ctcaacgatc ctgcaagagt gccatcttcc gcaaaaacag gccactctag atctacggcc
      481 tggaaacctt caacggctag aaacccctcg ctcaggaccg gcctgcctgt ttcatgaagt
      541 tttatgagtt gttcctggtc acctatccat tccccttcaa cgtaactgta atcccgagga
      601 tggatcaatc tcagcacaca atcgccgtct atgtaagaca catcgaccac ttcagagttc
      661 gcttcgtata gtctcttcag caacgatcga acttcttcgt catcgttcag tatctttctc
      721 atatcctcgg cacctttcga aagcgaagtg tcgagtctgt tgaaatgttc ctggatctcc
      781 ttctgcacct tactcaatag gagctcgggg cgtggatctc tcatcgtgga cagtagctcc
      841 cgtgcgatga cgaaagtcca gccggtcttc agcgatcgcc tgtaagccgc cagatggaga
      901 acgtcattac cggtgaagag aaagtcgctg ctatctctat cgagagttgc aagcctttcg
      961 gtaattccag gaaccggaac ttccagagag cccagaagca gggaggtatc gtccgatagt
     1021 gccagcgtgt tctgttcgtc gaaggcgtag aaatggactt ccggcagttg tttgaaatcc
     1081 gaaatcaatt cgaagttcca ttcatcgaag aaggccgaaa taccgacaaa accggatata
     1141 ttgtcctctt tacggacagg cgttgcgaag acgaccgaac gttttccggt agatctgctc
     1201 aaaacctggt atcccagaac ttctccgccc atcagaagcg cagaaaagta acccctgtcg
     1261 gaaaggttca ggccggtcca gtccctctgg acggtgtagt aacccccgtc aggttccacg
     1321 tacatggcca gtccttcata gccctggttg aagctgctaa ggagaggttt catcctcgac
     1381 cactctcccg atatcactcc gggagagcct gccagcaccc tcagtgaggt cattattccg
     1441 tggacgcgct cctcgaaaga agttaccagc agattcaaag cggtctggag aggtaccgac
     1501 gattgcgaga tcaggtcatc gactttttcg agtctggagg gatc
//
LOCUS       DAOOLY010000415         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_106008_length_1540_cov_4.288889, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000415 DAOOLY010000000
VERSION     DAOOLY010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106008_length_1540_cov_4.288889"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..625
                     /gene="rplU"
                     /locus_tag="PLD15_12730"
     CDS             302..625
                     /gene="rplU"
                     /locus_tag="PLD15_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPB64827.1"
                     /translation="MYAIIEASGRQYRVQEGMTLFTERQNGLSNGDEIVFDKVLLVRD
                     GEETKVGRPYVDGAKVVGKVVSHGRDKKVLVVKFGPRKNFDRVNGHRQWFTEVTIEKI
                     ELGVK"
     gene            627..872
                     /gene="rpmA"
                     /locus_tag="PLD15_12735"
     CDS             627..872
                     /gene="rpmA"
                     /locus_tag="PLD15_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPB64828.1"
                     /translation="MARKSAGRSNGRDSNPKYLGVKSGEASLVKAGTIIVRQRGTKIH
                     AGSNVGLGRDHTLFALIDGRVHFELRNNRKYASVYSE"
     gene            901..1425
                     /gene="rplM"
                     /locus_tag="PLD15_12740"
     CDS             901..1425
                     /gene="rplM"
                     /locus_tag="PLD15_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPB64829.1"
                     /translation="MKIQKTPLCRWAVTHSKVQTKRKGEIEKTLGYLVDHNGNKIERK
                     WYLVDATDVPLGRLAGQVAMVLMGKNKPLYTPHIDTGDFVIVINADKVKLTGKKLDQK
                     VYYRYSGYPGGIKERTARQMMDKTPDRVIRLAVKRMLPKKALGDKMLKKLKVYAGSEH
                     DHQAQKPELVVLVK"
     gene            1438..>1540
                     /gene="rpsI"
                     /locus_tag="PLD15_12745"
     CDS             1438..>1540
                     /gene="rpsI"
                     /locus_tag="PLD15_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB64830.1"
                     /translation="MADFVDYYGTGRRKTAVARVHLRPGSGKYLINGK"
BASE COUNT          443 a          316 c          446 g          335 t
ORIGIN      
        1 gtcaacccca ccgattttcg agaccggctc cttagccaat taggatacgc ctccattacc
       61 gtatttgatt atatcacggt ctgtaagagt gtcaacacgc ctcctgaagg aaatcacccc
      121 ttcttggcgg agcgggtccg ggtggtttcg taaagaaaaa gttaccggtg gagttcccat
      181 gagagaccag ttatgataga attttgatcg cgagcggttt ttctgtgcat gaaacgggtc
      241 aaaaatccct gtgggtacct tgtttcagca catctttagt gcatgaggag gtggctctta
      301 agtgtatgcc atcattgaag cctcgggaag acagtacaga gtccaggaag gcatgactct
      361 cttcaccgaa aggcagaatg gtctaagtaa tggagacgag atcgtcttcg acaaggtttt
      421 acttgtccgc gacggggaag aaacgaaagt tggcaggcct tacgtggacg gcgcaaaggt
      481 tgtaggcaag gttgtttcac acggtaggga caaaaaagta ctggttgtga aattcgggcc
      541 gcgtaagaac ttcgacagag tcaacggtca caggcagtgg ttcacagagg ttacgatcga
      601 aaagatcgaa ctgggggtga aatgatatgg cccgaaagag cgcaggaaga tccaacggta
      661 gagacagtaa cccgaagtac ctcggtgtga agagcggcga ggcttctctg gtgaaggctg
      721 gtacgataat agtcagacag aggggtacga agattcatgc cggcagcaac gtgggacttg
      781 gaagagatca cactctcttt gctctaatcg atggaagggt tcatttcgag ctcagaaaca
      841 accgtaagta cgccagtgtc tacagcgagt aggctgttct gttaaggagg attgtgtctt
      901 atgaaaatac agaaaacacc tttgtgcaga tgggcggtca cccactcgaa ggtgcaaacg
      961 aagagaaagg gcgagatcga gaagactctc ggttacctcg ttgatcacaa tggaaacaag
     1021 atagagagaa aatggtacct ggtagatgcc accgatgtcc ctcttggaag actcgcaggg
     1081 caggtagcca tggtcttgat gggaaagaac aaacctctct acactccaca catagatacc
     1141 ggcgacttcg tcatcgtgat aaacgccgac aaggttaagt tgaccggcaa aaagctggat
     1201 cagaaggtgt attacaggta ttccggctat ccgggcggta tcaaggagag aacggccagg
     1261 cagatgatgg ataagactcc cgacagagtc ataaggctgg ctgtgaagag aatgcttccc
     1321 aagaaggctc ttggagacaa aatgttgaag aagctgaagg tctatgcggg ttccgaacac
     1381 gaccaccaag cgcagaagcc cgagcttgta gtactggtga aatgaggggg ttgacgtatg
     1441 gctgattttg tagattatta cggaactggt agaaggaaga ctgccgtagc cagagttcat
     1501 ttaagaccgg gcagtggaaa gtatctgata aacggcaagg
//
LOCUS       DAOOLY010000416         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_109525_length_1508_cov_3.045423, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000416 DAOOLY010000000
VERSION     DAOOLY010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109525_length_1508_cov_3.045423"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLD15_12750"
     CDS             <1..659
                     /locus_tag="PLD15_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HPB64831.1"
                     /translation="RKRKIIGETFIEVFEGEASDIDADFLLQGTIYSDVIESAASSRT
                     SDKIKSHHNVGGLPENMKLKLLEPLRELFKDEVRALGKTLGISSKFIDRQPFPGPGLG
                     VRILGEVDAEKARILKEVDDIFLKTLEEYGEKGKIWQAFAVLLPVKSVGVKGDRRAYG
                     YVVALRAVHSAEGMTASWYEIPHAVLREISSRITSRVPEVGRVTYDITDKPPATIEWE
                     "
     gene            659..>1508
                     /locus_tag="PLD15_12755"
     CDS             659..>1508
                     /locus_tag="PLD15_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPB64832.1"
                     /translation="MSMFDKAYTFDDVLLVPGYSDILPREVDLSSRITRKLWLKVPFI
                     SAAMDTVTEAEMARAMALMGGMGVIHKNLSIEGQAEQVERVKKAPVDRERYPLCSLDS
                     FGRLMVGAAVGTGPGTVERIEAVVRAGIDVLAIDTAHGHSKRVIDLLSLVRELYPSLQ
                     IIAGNVVTSNGTLALIEAGADAVKVGIGPGSICTTRIVAGVGYPQLSAVMECSQVARE
                     RDIPIISDGGIRYSGDIVKALAAGASSVMMGNVFAGTKECPGKSTFVGGGEYKSYRGM
                     GSFTAMA"
BASE COUNT          398 a          323 c          447 g          340 t
ORIGIN      
        1 aacgcaagag gaagataatt ggcgagacct tcatagaagt cttcgaagga gaggcaagtg
       61 atatcgatgc tgacttcctg ctccagggaa ccatctattc cgacgtgata gaatcggctg
      121 cttcttcaag gacttccgac aaaatcaaaa gtcatcacaa tgtcggcgga cttcctgaaa
      181 acatgaagct gaagcttctc gaacctttgc gggaactctt caaagacgag gtcagagctc
      241 ttggaaaaac cctgggcata agttcgaagt tcatagacag acagcctttc cccggtccgg
      301 gactgggagt gaggatactg ggagaggtcg atgctgagaa agcgagaatt ctcaaagaag
      361 tcgatgacat tttcctcaag acactcgagg agtacggaga gaagggcaag atctggcagg
      421 ccttcgctgt attgttgcct gttaagagcg tcggggtcaa aggtgacagg agggcctacg
      481 gttacgttgt cgctttgagg gccgttcaca gcgccgaagg aatgacggcc tcctggtacg
      541 agattcctca cgcagtcttg agggagattt cttcaagaat aacctctcga gttccagaag
      601 tgggaagggt tacctacgat ataacagata aaccgccagc gaccatagag tgggaatgat
      661 gagcatgttc gataaagctt acacatttga tgacgttttg cttgttcctg gttacagcga
      721 tattctcccc agagaagtgg atctgagttc caggatcaca agaaagctct ggctaaaggt
      781 accgttcata tcggcggcta tggacacagt caccgaagcg gaaatggcca gagccatggc
      841 actaatgggc ggcatgggag tgatccacaa gaatctctcg atcgaggggc aagccgagca
      901 agtagagaga gtgaagaaag cacccgtaga tcgtgaaaga taccctttat gctctctcga
      961 ttcattcggc agactcatgg tgggagcagc cgttggcacg ggtccgggga cagtggaaag
     1021 gatcgaagcg gttgtaaggg ccggtatcga cgtgctggcg attgacacgg ctcacggtca
     1081 ttcgaaaaga gtcatagatc ttctttcttt ggtaagagag ctttatccct ctctccagat
     1141 cattgccgga aatgtggtga cttccaatgg aactctggcg ctcattgagg ctggtgccga
     1201 tgcagtaaag gttggaatag gaccaggttc catctgcacg acaagaatag tagcgggtgt
     1261 cggttatcct cagctttcgg ccgttatgga gtgttcgcag gttgcaagag agagagatat
     1321 tccgattatc tcagacgggg gaatacggta ttcgggagac atagtgaagg ctctcgctgc
     1381 cggagccagc agtgttatga tgggaaatgt cttcgccggg acgaaagagt gccccggaaa
     1441 gagtacattt gtaggtggtg gcgagtacaa atcctacagg gggatgggca gttttacggc
     1501 gatggctg
//
LOCUS       DAOOLY010000417         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_112187_length_1485_cov_3.195804, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000417 DAOOLY010000000
VERSION     DAOOLY010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112187_length_1485_cov_3.195804"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..659
                     /gene="hslV"
                     /locus_tag="PLD15_12760"
     CDS             111..659
                     /gene="hslV"
                     /locus_tag="PLD15_12760"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPB64833.1"
                     /translation="MIEMHGTTILVLRKNGKTVMAGDGQITLGETIMKGTARKVRKLG
                     DGRVLAGFAGSVADAMTLFEKFEEKFKESNSSLKRAAVNLAKEWRTNKILRNLQALLL
                     VADNETILLISGNGEVIEPDEEILAIGSGGSYALSAARALMRNTNLDAEMIAVQAMTI
                     ASEICIYTNSNFTVETLGGEGK"
     gene            656..>1485
                     /locus_tag="PLD15_12765"
     CDS             656..>1485
                     /locus_tag="PLD15_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB64834.1"
                     /translation="MTREELDLLTPRKIVEELDKYIVGQQMAKRAVAVAMRNRIRRQR
                     LPEEMKKDVIPKNILMMGPTGVGKTEIARRLAELTGSPFVKVEATRFTEVGYVGKNVE
                     SIIRELVDVGINMVKQEKIQEVEGKASFQVEERILESLVPGGQPKANGPKNIFELFQG
                     AQQNRPSQEEIERIRNRRDEYRERLRSGDLEDLDIEIELEDHSQPMIMIPGMEDMGID
                     MSGVLGGMVPKKVKRKKMRIGDARRAYPPAESEKLLDMHKVVSEAIESIEEYVNRQAH
                     "
BASE COUNT          456 a          313 c          435 g          281 t
ORIGIN      
        1 accgatctga gcgatcttcc gatgtcggcc agggccatgg gcctctcttt cgatcggacg
       61 ataggtgcga tcgtcgaagc ggccgaccgc aggaaccgga ggtgataaag atgattgaaa
      121 tgcacggaac gactatactc gtgttgagaa agaacggtaa aaccgtaatg gcaggtgatg
      181 gacagatcac tctgggtgaa accatcatga aaggtacagc ccgtaaggtg cgtaaactag
      241 gagacggaag agttcttgca gggttcgccg gctccgttgc cgacgcgatg acgctcttcg
      301 agaagttcga agaaaagttc aaggagagca attcaagcct gaagagagca gcggtaaatc
      361 tggccaaaga gtggcggacc aataagatcc ttagaaacct gcaggcactt cttcttgttg
      421 cagataatga gacgattctt ctgatttctg gtaacggcga ggttatcgaa cccgacgaag
      481 agattctggc gataggttcg ggcggttctt acgcgctctc cgctgctcga gctcttatgc
      541 gcaacacaaa cctcgatgca gagatgatag ccgttcaggc aatgactatc gcaagtgaga
      601 tatgcatata caccaactct aatttcacag tggaaactct cgggggtgaa ggtaagtgac
      661 gagagaggaa ctcgacctcc ttacaccgcg aaagatagta gaagagctgg acaaatacat
      721 agtaggacaa caaatggcca agagggccgt ggcagtcgcc atgcgcaaca gaatacgtcg
      781 tcagcgattg ccagaagaga tgaagaaaga cgtcataccc aaaaacatac ttatgatggg
      841 ccctaccgga gttggaaaga ccgaaatagc aagaaggctg gccgagctaa ctggatcgcc
      901 ctttgtcaag gtcgaagcca caagattcac cgaagtggga tatgtgggta aaaacgttga
      961 atcgataata cgcgaactgg tcgatgtcgg tataaacatg gtgaaacagg aaaagataca
     1021 ggaagtcgag gggaaagcct ctttccaggt agaagagaga atactggaat ctctggtgcc
     1081 cggaggtcag cctaaggcca acggcccgaa aaatatcttc gagcttttcc agggcgcaca
     1141 gcagaataga cccagtcagg aagagatcga aagaataaga aacaggagag atgagtacag
     1201 agagcgtctt cgctccggtg atcttgaaga cctggatata gagatcgaac tggaggacca
     1261 cagtcagcca atgataatga taccgggcat ggaggacatg ggtatagata tgtcgggtgt
     1321 cctgggaggc atggtcccaa agaaagtgaa gaggaagaag atgaggatag gcgatgcgag
     1381 aagggcctat cccccggctg agtcggaaaa gctgttggac atgcacaagg ttgtgtccga
     1441 ggcgatagag agcatcgagg aatacgtcaa cagacaagcg cataa
//
LOCUS       DAOOLY010000418         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_112779_length_1480_cov_3.058947, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000418 DAOOLY010000000
VERSION     DAOOLY010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112779_length_1480_cov_3.058947"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..913
                     /locus_tag="PLD15_12770"
     CDS             128..913
                     /locus_tag="PLD15_12770"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB64835.1"
                     /translation="MELLADIGNTTTVFGLQKGDSIISIWRLPSGRLETEDELFAILE
                     GLLKSKGSSIGEIDGFCVASVVPRLNRSIQYFAAKYLKEPAVFLKPDRFVSLELKADN
                     PGEIGADRLANVISASIHYGANAVVIDVGTAITIDVLKENAFVGGSILPGPSTAMSAL
                     FSKTAKLPEVELFFEDHYLGTNTEDNIRIGIVNGTYFALAGIVENMLKVFDGRVDVIG
                     TGGNVEMFMVEKGFITINDPDLTLKGIKAYYDRVKESKKNTAG"
     gene            873..>1480
                     /locus_tag="PLD15_12775"
     CDS             873..>1480
                     /locus_tag="PLD15_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPB64836.1"
                     /translation="MTESRKAKRILLVNPWIEDVSAYDYWLKPVGLLYISSILKDSGI
                     ETVLVDCLDRYDADFLFSGGESREKYFGTGKFHEVVIPKPESMKDIPRKFKRFGIPEE
                     LLRMKLRSAGDVAGVFVTSMMTYWYTGVVDTIRVIHEELPAARIVLGGVYATLLPDHA
                     AVKCGADFLCPGTGVAPVKRALEYLGIDVLPREDWFTALDPD"
BASE COUNT          368 a          338 c          425 g          349 t
ORIGIN      
        1 tcatcacatt ttcagtcgat caagtggaga gaaatattaa aagaatgtga aaccgtactc
       61 cggcgaataa aattcggaga tagaacggaa taatgctgca tggtagaatt gtcagggagg
      121 cgattcaatg gaacttctgg ccgatatcgg caatactacg actgtatttg gattgcaaaa
      181 aggcgactcg ataatttcga tatggagact cccttcgggg agactcgaaa cagaggatga
      241 gctcttcgcg attctcgaag gtcttctaaa aagcaagggt tcatctatcg gcgagatcga
      301 cggcttctgc gtggccagtg ttgtgccgag gctcaacagg tcgatccagt atttcgccgc
      361 gaagtatctc aaagagccgg cggtctttct caagccggac cggttcgtat ctctggaact
      421 gaaggccgac aatccgggtg agataggcgc cgacagactg gccaatgtga tcagcgcttc
      481 cattcattac ggggcgaacg cggttgtcat agatgttgga actgctataa cgatagatgt
      541 attgaaggaa aacgcctttg tcggaggttc tatactcccg ggcccttcga cggctatgtc
      601 cgccctcttc tccaaaaccg cgaaacttcc agaggtagaa ctctttttcg aagatcatta
      661 tctcggaacc aacacggaag acaatataag gatcgggata gtcaacggca cttactttgc
      721 actggctggc atcgttgaaa acatgctgaa agtcttcgac ggcagggtag acgtgatagg
      781 aactggcggc aatgtcgaga tgttcatggt cgagaagggc ttcatcacga tcaacgatcc
      841 agacctgacg ctgaagggga tcaaggcgta ctatgacaga gtcaaggaaa gcaaaaagaa
      901 tactgctggt taatccctgg attgaagatg tgtctgctta cgactactgg ttgaaaccgg
      961 tgggcctgct gtacatctct tcgatcctga aagattcggg aatcgagacc gttctcgtgg
     1021 attgccttga tagatacgat gcggactttc tcttctccgg cggagagagc cgggagaagt
     1081 acttcgggac gggtaagttt catgaagtgg tgatccccaa gccggagtcc atgaaggata
     1141 tacccaggaa gttcaagcgt ttcggaattc ccgaagagct gctcaggatg aagctccgtt
     1201 cggcgggcga tgtggccggg gttttcgtga cttcgatgat gacttactgg tacaccgggg
     1261 tggtcgatac gatacgtgtg atccacgagg agttgccggc cgccaggata gttctgggag
     1321 gggtttacgc aacgcttctg cccgatcacg cggccgtcaa gtgcggtgcc gatttcctct
     1381 gtccggggac cggtgtcgct ccggttaaaa gggcgctcga atacctcggc atagacgtat
     1441 tgccgcgcga agattggttt acggcgctcg accctgatta
//
LOCUS       DAOOLY010000419         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_113103_length_1477_cov_3.777075, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000419 DAOOLY010000000
VERSION     DAOOLY010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113103_length_1477_cov_3.777075"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..>1477
                     /locus_tag="PLD15_12780"
     CDS             162..>1477
                     /locus_tag="PLD15_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB64837.1"
                     /translation="MKRFFQILVLALLAVTLLAGSPGKLVMAIETEPVGLDPTLVTAF
                     ASHRVLENVYDGLLRYDESMNLVPNLAESFEVVDPYTILFKLRDGVKFHSGDPLTSED
                     VIFTFERLMNPDTKAPAATYYKEVSSIKVVDGNKIEFKLTIPMAASLLPNFAGVNSSI
                     LSKKFVESGANLQLSANGTGPFELADYVAGNYITLKRNTEYFIEGLPYLDEIKMMIMP
                     EEVTRVSALRNGDVDLARINEPLSLNQLPTDRFKIHRNAVLSYYLLGINTTRGPLSNP
                     KVRNALNYAINREMIVKLVAFDEGAVTGPLNPELEFWAMKPSQFSEYTYNPAKARELL
                     TEAGYPNGFEFEIVASQRYSFDKVAQVIQAQLAEVGVRAKINLVEWGIFISKWRESDF
                     DSFISLNSGSIDPDIQFNRTFHTGGSTNVFLYSNPEVDALLDRGRS"
BASE COUNT          400 a          361 c          376 g          340 t
ORIGIN      
        1 agggatatag agatttcgca ggtcgatgag ataagggtct ccggaagact tctcgccata
       61 agggcaggaa acagactgct aatttacgat gttctatcgg caggtaaccc agtgcttctg
      121 aatgaataca attgagtgcg ctactacaag gaggtggctg tatgaaaagg ttctttcaga
      181 ttttggttct ggctttgtta gctgttacgt tattagcagg ctcccctggg aagctggtga
      241 tggccatcga aaccgaaccg gttggcctcg atcctacact ggtcacggct ttcgcttcgc
      301 acagggtgct ggaaaacgtt tatgacggtc tgttgaggta cgatgaaagt atgaacctgg
      361 taccaaatct tgccgaaagc ttcgaagtgg ttgatccgta cactattctc ttcaagctga
      421 gagatggtgt gaaattccat agcggagatc cattaacctc ggaggacgtg attttcacgt
      481 ttgaaaggct aatgaacccc gacaccaagg ccccggccgc gacctactac aaggaagtca
      541 gttcgataaa ggtcgttgac ggtaacaaga tcgagttcaa gctcaccatc ccaatggccg
      601 cctcgctttt gccgaacttc gccggtgtca acagctcaat cctctccaag aagtttgtgg
      661 aatcgggagc caatctccag ctctcggcca acggaacagg acccttcgaa cttgccgatt
      721 atgtagcggg caactacata actctcaaga gaaacacgga gtatttcatc gagggccttc
      781 cttacctgga cgagatcaaa atgatgatca tgcccgaaga ggtgaccagg gtttcggcac
      841 tgagaaacgg tgacgttgat ctggcaagaa taaacgagcc gctcagcttg aatcaactac
      901 cgacagacag attcaagatc cacagaaatg cggtgctcag ctattacctt ctgggaataa
      961 acaccacgag ggggccgctc agcaacccca aggtgagaaa tgccctgaat tacgctatca
     1021 atagagaaat gatcgtcaaa ctcgtcgcct tcgacgaggg tgcggtgacc ggtccgctca
     1081 atcccgaatt ggagttctgg gcgatgaaac cctctcaatt cagtgaatac acatacaacc
     1141 ccgcaaaggc tagagagctc ctaaccgaag ccggatatcc taacggtttt gagttcgaga
     1201 tagtggcctc ccagagatac agtttcgaca aagttgccca ggtcattcag gcgcagcttg
     1261 cagaggtcgg tgtcagagct aagataaacc ttgtggagtg gggtatattt ataagcaaat
     1321 ggagagagag cgacttcgac tccttcattt cactcaacag tggttctatc gatcccgaca
     1381 tacagttcaa cagaactttc cacacaggtg gttctacgaa cgtcttcctc tatagcaacc
     1441 ccgaggttga cgccctgctc gacaggggaa gaagcga
//
LOCUS       DAOOLY010000420         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_117565_length_1441_cov_4.155844, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000420 DAOOLY010000000
VERSION     DAOOLY010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117565_length_1441_cov_4.155844"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..759)
                     /locus_tag="PLD15_12785"
     CDS             complement(118..759)
                     /locus_tag="PLD15_12785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPB64838.1"
                     /translation="MEGRIPLIGEDFPAMKVKTTHGVMNLPEAYKGKWFVLFSHPGDF
                     TPVCTTEFVGFQKRYDAFKELNCELIGLSIDQVFSHIKWAEWIEEKLGVKIEFPIIAD
                     DMGEVGKTLGLIHPAKGSNTVRAVFIIDPKGKIRAILYYPQELGRNMDEILRMVKGFH
                     VVDGKGVALPADWPNSDLVGDHVIIPPASDVKTAEERKNKEGCYDWWFCHKSL"
     gene            complement(876..1199)
                     /locus_tag="PLD15_12790"
     CDS             complement(876..1199)
                     /locus_tag="PLD15_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64839.1"
                     /translation="MYIVCDLRYTVSESRSSEALKMILETLSKKLLLGARGDALNMKA
                     EDSSVLEVQIMALKGECSFEEVIPRINEYLTLISKALEEKFPDYRVVSSYIYPDHEQN
                     PCLLC"
BASE COUNT          380 a          354 c          289 g          418 t
ORIGIN      
        1 ctatatcgac atactaaaca acaaaaagct tctccagaac ataatcgatt ggtagaaagc
       61 gccgcactct acgaaaagaa gcgggcaata tgcccgcttc tctatttata aatgttctta
      121 aagactcttg tggcagaacc accagtcgta acagccttcc ttgttctttc tctcttcggc
      181 cgtctttaca tctgacgcgg gcggaattat cacatgatcg cctacgaggt cgctgtttgg
      241 ccagtcggca ggaagcgcta cgcctttgcc gtcaactaca tggaagccct tcaccattct
      301 cagtatctca tccatgtttc ttccgagttc ctgggggtag taaaggatcg ctctgatctt
      361 tcccttggga tctattatga agacggctct gacggtgttg gaaccctttg ccggatgtat
      421 gagacccagt gttttgccaa cttcgcccat atcgtcggca attatcggga actcgatctt
      481 gactccgagc ttttcttcaa tccattcggc ccatttgata tgcgaaaaga cctggtctat
      541 gctcaggccg atgagctcac agttcaattc tttgaaagcg tcgtatctct tctgaaaacc
      601 cacgaattcc gtggtgcaaa caggggtaaa atctcccgga tggctgaaaa gcacgaacca
      661 cttacccttg tatgcttcgg gtaaattcat taccccatgt gttgttttga ccttcatcgc
      721 tggaaagtct tcaccgatca atggaattct tccttccata aagcacctcc tatttagaat
      781 aattattatc tgtaatatca tgattatata cgtctttaca ttccctgtca agcataaata
      841 acaaaagggc ctttcggccc cttttttgta tctcttcaac agagtagaca gggattttgc
      901 tcgtgatctg gataaatata agagctgacc actctgtaat ctgggaactt ttcttccagt
      961 gcttttgaaa tgagtgtcag gtactcgttg attctcggta ttacttcttc aaaggaacac
     1021 tcgcctttca aggccatgat ctgcacttcg agaacactgc tgtcctcggc cttcatgttg
     1081 agagcatccc cccgggctcc caggagcaac ttctttgaga gcgtctcgag tatcatcttc
     1141 aaagcttctg aggatctact ttccgaaaca gtgtacctca aatcgcatac aatgtacatg
     1201 gcgctccctc cttcgtctgg tttcttcaat aatgtttgta actaacacag gaaggtacct
     1261 aacggtagat ccgcatttct ggaaaagcct gttttgaaag accgaaaacc tgagtatttg
     1321 acccgaaaaa gtctgacccg gtttgaatat tatactccat atactaatta cagaggttac
     1381 tcatatagac cgtatccaat aaataggcga ggaaagaaat aaggcaaaag aaaagggact
     1441 c
//
LOCUS       DAOOLY010000421         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_117947_length_1438_cov_4.796819, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000421 DAOOLY010000000
VERSION     DAOOLY010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117947_length_1438_cov_4.796819"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLD15_12795"
     CDS             complement(<1..490)
                     /locus_tag="PLD15_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64840.1"
                     /translation="MEIIHTPFGIDDPYFIVQGCEREPREPLEGGCVKVRFVTDPMVV
                     GQRAWIEVQSDSRRYKRRAQYQLNHGGKAHWEVNIDGENAGVTVRYRFIAGRGPSTYV
                     TSEWYEYTVCRWIEAKRFLSLKNDRLAVNRSDGTSCDCLKEAYVLTDGKELYDLKIIF
                     ERE"
     gene            complement(504..>1438)
                     /locus_tag="PLD15_12800"
     CDS             complement(504..>1438)
                     /locus_tag="PLD15_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB64841.1"
                     /translation="IGVGYLDYSLTDLSGSVLASERKLMENTVPQEVVSATGEILGKW
                     SELIGERLLGIGIAVSGIVDFLRGVVKNSFLLNWNDIPIARMISAGCAAKAFVMNDVD
                     SFALAQFWKGNVDNHKNCVFITLGIGIGGAVAIDGKLHLTGAGSGEFGHMTIEHNGNL
                     CTCGSRGCLEAHASFEVLARKLFSITASPELKELYGSLRATETSEIHFLKRALEIDRA
                     STESVFDEYATDIGIALKNLVNIFAPDYVLIGGEALEFSALFLEKSINYAKKNAFSSL
                     ADAVKFDIDIIGEKAWTLGCIYRVIESELFTVGR"
BASE COUNT          330 a          411 c          316 g          381 t
ORIGIN      
        1 cttctctttc aaaaatgatt ttcaggtcgt acagttcctt cccgtccgtt aagacatatg
       61 cctccttgag gcagtcacag ctggtgccat cacttcgatt caccgccagc ctgtcgtttt
      121 taagggaaag aaatctcttt gcttctatcc acctgcaaac ggtgtattcg taccactctg
      181 aagtaacgta agtggagggt ccacgaccgg cgatgaatct atacctgacc gttactcccg
      241 cattctcgcc gtctatattt acttcccagt gagccttgcc gccatgatta agctgatact
      301 gggcacgcct tttatatctc ctgctatcgc tctggacttc gatccaggct ctctgaccga
      361 cgaccatggg gtcggtcacg aacctgacct tcacacaacc accctccagg ggctcacgtg
      421 gttctctttc gcacccttga actatgaaat acgggtcgtc tatcccgaac ggagtgtgaa
      481 ttatctccat gttcctcccc ctttcatctg ccaacagtga agagctcgct ctctatcact
      541 ctgtagatac aacccaaagt ccaggccttc tcgccgatta tgtcaatgtc gaacttcacg
      601 gcatctgcca ggctggagaa ggcgtttttc tttgcatagt ttatcgattt ctcaagaaaa
      661 agggccgaaa actcgagggc ctccccacct attagcacgt agtcgggagc gaatatattc
      721 acaagattct tcagggcgat tccaatatcg gtcgcgtatt cgtcgaatac gctttcggtg
      781 cttgccctgt cgatctccag agctcgtttc aagaaatgga tttccgaggt ttccgttgcc
      841 cttaaagaac cgtacaactc ttttagctcc ggggaggcgg ttattgagaa tagttttcgc
      901 gctagaactt cgaaagaggc gtgagcctcc agacagccac gactaccgca tgtgcagagg
      961 tttccgttgt gttcgatcgt catgtggccg aattcgccgc tcccggcgcc tgtcagatga
     1021 agctttccgt cgatcgccac ggcaccgcct ataccgattc ccagagtgat gaagacacag
     1081 ttcttgtgat tatcgacatt tcctttccag aactgggcca gcgcgaagga atcaacgtcg
     1141 ttcataacga aggctttagc ggcacagccc gccgaaatca ttctggctat gggtatgtca
     1201 ttccagttga gcaggaagga gttctttacg actcctctga gaaaatccac aatacctgaa
     1261 acggcaatcc cgattcccag aagcctctcg cctataagct cgctccattt tcccagaatt
     1321 tcacccgttg ccgataccac ctcttgggga accgtgttct ccataagctt tctctcgcta
     1381 gccagaacag agcccgaaag atcggtcagc gaataatcga gatatccaac gcctattt
//
LOCUS       DAOOLY010000422         1429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_119164_length_1429_cov_4.857351, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000422 DAOOLY010000000
VERSION     DAOOLY010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119164_length_1429_cov_4.857351"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLD15_12805"
     CDS             <1..861
                     /locus_tag="PLD15_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPB64842.1"
                     /translation="TTTGFNIDRQLVSDLLGFERPVPNSYQAIVTSHWLTYPSMWFKS
                     ILYDITRLMADMGHKASCEVGMFSFPDELVQVSSIMPQKRNPVIIEHIRIQSGMAAGD
                     FQGLIDLFHNVPYQDVNEVADAPVTVFTRGCETLSGLLELLEETLLKVSVDERRVDDI
                     AISTGTTTTELADELVRREAISFRTAHGVTSAFVKSGYSFEALRGKFSELVGRALDMS
                     DSELRNVLSPRHFVQVRNIPGGPSVSAMESVINLMEEKVKIITDSLDGLVKKIVESEE
                     HLSVEFNGLG"
     gene            874..>1429
                     /locus_tag="PLD15_12810"
     CDS             874..>1429
                     /locus_tag="PLD15_12810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPB64843.1"
                     /translation="MIPAGKIIERLEEAISRANTEMDPAIVELLDHYEGPFSSVLREN
                     AIAAKETGLPICQDTGFLEFFVFQGNEVALEEPIIETLNSAVKKVYNDKPYRYSIVSD
                     PLKRVNTRDNTPVVCHLFPVRGDRLEIRFLVKGGGSENLSRLFMLNPTATVDDLVRTV
                     VESLKESAARGCPPLKVGIGIGGSS"
BASE COUNT          370 a          353 c          392 g          314 t
ORIGIN      
        1 acaacgacag gcttcaacat agacaggcag ctggtctcgg atctgctcgg attcgaaagg
       61 ccggtcccga actcttatca ggcaatcgtt acctcgcact ggctaaccta tccgtcgatg
      121 tggttcaaga gcattctcta cgatataact cgcctcatgg ccgacatggg gcataaagcc
      181 tcttgcgaag tcggaatgtt cagctttccc gatgaactcg tgcaggtttc cagcataatg
      241 cctcagaaac gaaacccggt tataatcgag catataagaa tccagtccgg gatggccgcc
      301 ggcgactttc agggcctgat tgacctcttc cacaacgtgc cataccagga tgtgaacgaa
      361 gtggccgatg ctccggtcac cgtcttcacc agaggctgcg aaacgctttc gggacttctg
      421 gagctgctgg aagagacgct tctgaaagtc tccgtggatg agcggagggt cgacgatata
      481 gcgatctcca caggtactac aacaacggaa ctggccgacg agctcgtaag gagagaagcc
      541 atctctttca ggacggctca cggcgtgacg agcgctttcg ttaaatcggg atactctttc
      601 gaagccctcc gcggaaagtt ttctgaactg gtgggaagag cccttgatat gagcgattcc
      661 gagctcagaa atgttctctc tccccggcat ttcgtacagg tacgtaacat ccctggagga
      721 ccctctgttt ccgcgatgga aagtgttata aatttgatgg aagaaaaagt gaaaataatc
      781 acagactcgc tggacggact agtcaaaaaa atcgtagaga gcgaagaaca tctatcggta
      841 gagttcaacg gtctgggttg acggggtgta actttgattc cggcaggaaa aattatcgaa
      901 agactcgaag aggctatttc gagggcgaac acggagatgg atccggcgat cgtcgagttg
      961 ctcgaccact acgaaggacc cttttcgagc gtgttgaggg aaaacgccat cgccgcaaaa
     1021 gaaacggggc tcccgatatg tcaggataca ggttttctcg agtttttcgt ctttcagggc
     1081 aacgaagtcg ctctggaaga accgatcatc gaaacactga attcggcagt caaaaaggtt
     1141 tacaacgaca aaccctacag atactccata gtgtccgatc cccttaagag ggtaaatacc
     1201 agagacaaca cgccggtcgt atgccacctg tttccggtga ggggagacag gctagaaatc
     1261 cgctttctcg ttaagggagg gggaagcgag aatctctccc ggcttttcat gttgaatccc
     1321 accgcaaccg tggacgatct ggtccggacc gtcgtcgaat cgctcaagga gagtgcggcc
     1381 agaggttgtc caccgttgaa agtgggtata gggatcggcg ggagttcgg
//
LOCUS       DAOOLY010000423         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_121029_length_1415_cov_4.436765, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000423 DAOOLY010000000
VERSION     DAOOLY010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121029_length_1415_cov_4.436765"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1415)
                     /locus_tag="PLD15_12815"
     CDS             complement(<1..>1415)
                     /locus_tag="PLD15_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64844.1"
                     /translation="LNELDRVLTQARQHAVDTDPLDRELIRLKELRTNLNRYVEALHR
                     KRSNLGISAFEVHGKLAELDGVKSLSFRYEGTGKLDREEMGVILHDLDQVKRFADIYR
                     DYRCSPLNAVKADGPDALLIIRIRESLEALNETIQRLDSLGGCEPEDVGRLVESLSRI
                     DTPLNVIINRKDLFHPTEDKTIESVSVLIGLIGEIERTLPSKYRGIFLQAKRSLQELV
                     SSYEALIEKRTRVGEITRSQRNIDTMKPFEETVNLLTRYRAGILSLDHDELIGKFKGQ
                     YRSWFGRLLGRYRKKVELLRSLTLNGLPDISKIVEDLEMASRVASRVIPPGCVFEESD
                     LLELLLDSRERLLIHYRQYDKVKAGLEQLAGTLTELAELLSFVGLIRVHKERRSDIPA
                     MTADLWLDSVSIEKERLKTQLMDISTIVDISVIARGGSISDYKRTASFIRELTTASIH
                     LQEWLSLKKSVKNLKRIGLED"
BASE COUNT          324 a          353 c          340 g          398 t
ORIGIN      
        1 agtcctccag tccgatgcgc ttaaggtttt tcacgctctt cttaagtgaa agccactcct
       61 ggaggtgaat cgaagctgtc gtcaattcgc ggatgaaact ggcggtacgt ttgtaatcgc
      121 tgatcgatcc tccccgggct ataacagaga tatctacaat ggtcgaaata tccataagtt
      181 gagttttcaa tctttccttc tctatagata cggagtccag ccacagatct gcagtcatcg
      241 ccggtatatc tgatctacgc tctttgtgaa cacggatcag tccgacaaac gagaggagtt
      301 cggccagctc agttaacgtt ccagcaagct gttcgagccc ggctttcact ttgtcgtact
      361 gccggtaatg aataaggagg cgctctctgg agtcgagcaa aagctccagc agatcgctct
      421 cttcaaaaac gcaaccgggt ggtataacgc gagaggcaac tctggaggcc atttcaaggt
      481 cttcaactat cttcgaaatg tccggcagac catttaaagt cagagaacga agcagctcta
      541 ctttcttgcg gtaccgccct aataaccttc caaaccagct acggtactgg cctttgaact
      601 tgccgatgag ttcatcgtga tcgagcgaga gaatgccggc acgatacctg gtgaggagat
      661 ttactgtttc ttcgaatggt ttcattgtgt cgatgtttct ctgacttctg gtgatctcgc
      721 ctactctcgt tcttttttca atgagcgctt cgtaagaaga gaccagctcc tgaagacttc
      781 gtttggcctg gaggaatatc ccgcggtatt tagaagggag agtcctttct atttccccga
      841 tgagtccaat taacacggaa accgattcga tagtcttatc ctcggtcggg tggaagagat
      901 cttttcgatt tattatcaca tttagcggag tgtctattct ggaaagactt tcgacgagcc
      961 ttccgacatc ttccggttca caccctccga ggctgtccag tctttgtatc gtctcgttca
     1021 gcgcttcaag cgattctctg atacgtataa tcaggagcgc atctggaccg tcggccttga
     1081 ccgcgttgag tgggctgcag cggtaatcgc ggtagatatc tgcaaaacgc ttcacctgat
     1141 cgagatcatg cagaataacg cccatttcct ctctgtcaag tttgcctgta ccttcgtatc
     1201 tgaaactgag agatttcacg ccatcgagtt cggcgagttt tccatgaact tcgaaagcgg
     1261 agatcccgag attcgatcgt ttcctgtgaa gggcctccac ataacggttg agatttgtcc
     1321 tcagctcttt aagcctgatc aattccctgt ctagagggtc agtatcgact gcgtgttgtc
     1381 tggcctgcgt gagaactcta tcgagttcgt tgagg
//
LOCUS       DAOOLY010000424         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_121055_length_1415_cov_3.480882, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000424 DAOOLY010000000
VERSION     DAOOLY010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121055_length_1415_cov_3.480882"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1415)
                     /locus_tag="PLD15_12820"
     CDS             complement(<1..>1415)
                     /locus_tag="PLD15_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPB64845.1"
                     /translation="KDGYIDIKVYSLDQEDLTGALTIADVRISGDPLYAIRQTVKENQ
                     RLTLPVDSMKGLRLLVVTSESKTAKVMTSYRTVGAQLLKHEKGAMLRLWTREDSIFIK
                     DFSLYRLRDGSLIGKAEDGLFVFDSLPESGEAVLVQSPYGSLLWKPGEYDYYLPSRYV
                     SMTFTDRPAYKAGETVNFRSFIREITPEGYKIPKLGPVEVEITDPLNRSVYKENLTPD
                     ELGSIDSSFKTYSEITRGSYRIIVRWEGNEDYYYFQIADYKKPTFFTTAEATAKVFKK
                     GESVSIDVKSQYYFGDPVSLGKVDYTIYKGGQYIDNGTARLDGVGETVIGYNGPLDPG
                     YYYAVVTVSDDTGMQSRTTVDFKIVQGTFGFVTEYTLNNKQLMAKIETRLNDDTPVSK
                     TIEVKAWYEQSVTLIDQGKAVEKKVKINVVRREITTGSDGKAEFTVDLAEVPDDNLVY
                     VELSGAPSGEAEVTDRYSIYVG"
BASE COUNT          274 a          393 c          362 g          386 t
ORIGIN      
        1 cctacgtata tggagtatct gtcggtcact tccgcttcgc cggagggcgc gccgctcagc
       61 tctacgtaca ccagattgtc gtcgggcacc tctgcgagat ctacggtgaa ctctgctttg
      121 ccgtcgcttc cggtggtgat ctcccttctg acaacgttga tcttgacttt cttctctacg
      181 gctttgccct ggtcgatcag cgttacgctc tgttcgtacc aggccttcac ctcaattgtt
      241 ttggacaccg gcgtgtcgtc gttcagccgt gtctctattt tggccatcag ctgtttgttg
      301 ttgagcgtgt attctgtgac gaagccgaag gtgccctgca cgatcttgaa atccaccgtg
      361 gtgcgcgact gcattcccgt atcgtcggac acagtgacga ccgcgtagta gtaaccggga
      421 tccagcggac cgttgtagcc tatgaccgtc tcgccgacac cgtcgagtct ggccgtcccg
      481 ttgtctatgt actggccgcc tttgtatatc gtgtaatcga cctttcccag cgacaccgga
      541 tcgccgaaat agtactggga tttcacgtct atgctgacgg actcgccttt cttgaagact
      601 ttggcggtcg cttcggccgt ggtgaagaag gtgggcttct tataatcggc tatctggaag
      661 tagtagtaat cttcgttccc ctcccagcgc actatgattc tgtacgaacc cctggtgatc
      721 tccgaataag ttttgaaaga tgagtcgata gaacccagtt cgtcgggagt gaggttttct
      781 ttgtacaccg agcggttgag aggatcggtt atctcgacct ctaccggacc cagtttcggg
      841 atcttataac cctccggagt gatctccctt atgaaagacc tgaagtttac ggtctctccg
      901 gctttgtacg ccggtctgtc ggtgaaagtc atggatacgt atctggaagg caggtagtaa
      961 tcgtactccc cgggcttcca gaggagcgaa ccgtaaggcg actgaaccag aacggcctcg
     1021 ccgctctccg gaagcgaatc gaacacgaag agcccgtctt cggccttccc tattagagag
     1081 ccgtccctga gtctgtaaag agagaaatct ttgataaaga tggaatcctc ccttgtccag
     1141 agcctcaaca tcgctccttt ttcgtgtttg agcaactgcg cccccacggt ccggtagcta
     1201 gtcatcactt tagccgtttt cgattcggaa gtgaccacca gtagcctgag tcctttcatg
     1261 ctatctaccg gcagggtcaa cctctgattc tctttaacgg tctgcctgat cgcatagagc
     1321 ggatcgcccg agatcctgac atctgcaata gtcaaggctc cggtcaggtc ttcttgatcg
     1381 agactgtaca ccttgatgtc gatgtagcca tcttt
//
LOCUS       DAOOLY010000425         1406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_122262_length_1406_cov_3.403405, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000425 DAOOLY010000000
VERSION     DAOOLY010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122262_length_1406_cov_3.403405"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLD15_12825"
     CDS             complement(<1..1302)
                     /locus_tag="PLD15_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPB64846.1"
                     /translation="MIRRFSELPLNKRLSAYIMLIALGILLAVYLTNYFVSKSIILDG
                     IEEDSRNLAEAKVNTMETFMTGVEKIPEAISSLVKNYPYDSARVSTVVRELLRKNPGI
                     FGMCIAYEPVFRPDSVPFAPYFYREKDVIAYTDLAKDEYRYEHWDWYQIPYTIRKSLW
                     SEPYYDKGGGENLMITYSVPIIDSTSGRVFGIVTADVDLKLLRDIVSQIRIKENGFAF
                     AISRFGTFISHRNGDYILNETIFSLAESKDDDELELLGQKMTGGESGFQAVVDPSSGD
                     DYRLYFIPLKSAGWSVGIMIPESDLFSGLYFLSNMILLVGFIGASILAVFIYFVIEKT
                     TKPVSTISDVVALVANGNLKAAAAKLQHETANHKDVDNESASSHDEIIKLWHYIRKMI
                     LDLSGLSIRIKSAGNYLHSSAGTLSDAAAFLNNTFTEQDTAN"
BASE COUNT          339 a          316 c          374 g          377 t
ORIGIN      
        1 gttggcggta tcctgctccg tgaatgtatt gttcagaaat gctgccgcgt ctgaaagcgt
       61 tccggccgaa gaatggaggt agttgcccgc agactttatt cttattgaga gaccgctcag
      121 atcaagaatc attttcctga tgtagtgcca caatttgatt atttcatcat gtgaagaagc
      181 ggattcatta tccacgtcct tgtggtttgc agtctcatgc tggagtttgg ctgcggcggc
      241 ctttagattg ccgttggcca cgagtgccac cacatcagag atggtggaaa caggttttgt
      301 ggttttttct ataacaaaat agatgaatac cgcgaggatg gatgcaccga taaacccgac
      361 cagcaggatc atgttgctga ggaagtacag ccctgagaag aggtcacttt cggggatcat
      421 aattccgacc gaccatcctg ccgattttag gggtataaaa tagagtctgt agtcgtcacc
      481 tgaagagggg tcgacaacag cctgaaaacc gctttcacca ccggtcatct tctgcccgag
      541 caactcgagt tcatcatcat ccttcgactc cgcgagggag aagatggttt cattgaggat
      601 atagtcgccg ttccggtggg agatgaaggt gccgaagcgg ctgatagcga aggcgaaacc
      661 gttctctttg atcctgatct gggagactat gtctcgcagc aatttgaggt caacatcagc
      721 tgtaacgatg ccaaatacgc ggcccgaagt ggagtcgatt atcggtaccg aataggtgat
      781 catgaggttc tcgccgccgc ccttgtcata gtatggctca gaccagaggg attttcggat
      841 ggtgtagggg atctggtacc agtcccagtg ttcataacgg tattcatcct tggcgaggtc
      901 ggtgtatgct ataacatctt tttcccggta gaaatacggg gcaaaaggaa ccgaatcggg
      961 tctgaaaacc ggttcgtagg cgatacacat accgaatatt ccgggatttt tccggagcag
     1021 ttcccttact actgtggaca cccttgctga atcatagggg taatttttta cgagactgga
     1081 gatcgcttca gggatcttct ctactcccgt catgaaggtt tccatcgtgt tcactttggc
     1141 ttcagcaagg ttacgggagt cttcttcgat accgtcgagg atgatcgatt tggatacgaa
     1201 atagtttgtc agatacacgg ccagaagaat gccgagcgcg atgagcatga tgtatgcaga
     1261 cagcctttta ttcaggggga gttcagagaa tcttctgatc attttaccac ctgcacatag
     1321 aagttgtaat acttaatgtt cccttcgatc ctgccggcgt ctttgagaac ctggcagacc
     1381 tctttacagg cggagcagga cggttt
//
LOCUS       DAOOLY010000426         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_122851_length_1402_cov_2.960653, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000426 DAOOLY010000000
VERSION     DAOOLY010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122851_length_1402_cov_2.960653"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLD15_12830"
     CDS             <1..978
                     /locus_tag="PLD15_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein"
                     /protein_id="HPB64847.1"
                     /translation="NSVGELAGNNILNYIRKMEIDQQKKISPEINGLETFGIDKDDLL
                     IFIATNTEVSKACMEGLTEFYRSRGISVVTRVVEGLTDDSSTAFKKGIADYVDLIISL
                     FDKGEQWYYENFFNATSGYRSLIPYTTIVAAVFDSRVFYLYENSPFLLDLPPFPINFD
                     FGIMEQHSAFFEKVENDCVPSREAIEEFGYELFSKVVPQVLLEEDGLVYLSNFGKILW
                     GRYTVSNPEIYFSDSAQKFINRDPVYLDYVRKMLRGEAEAEHYLHEYVGDAQCYKFPA
                     KGVRIFYIREGDGPFRIAAILPNHDEYERYIKTKQHESSLSYTKTRINL"
     gene            complement(1199..>1402)
                     /locus_tag="PLD15_12835"
     CDS             complement(1199..>1402)
                     /locus_tag="PLD15_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HPB64848.1"
                     /translation="VDREYLKRTGRKSNLRLKIKEDTEVFGSMVPVKITGKKEEIAFL
                     YHCGLGQKNSLGMGMIELAGKRF"
BASE COUNT          383 a          327 c          319 g          373 t
ORIGIN      
        1 aattcagttg gagagctcgc gggcaacaat attcttaatt acatcaggaa gatggaaata
       61 gatcagcaga agaagatatc cccggaaatc aacgggctgg agactttcgg aatagacaaa
      121 gatgatctcc ttatattcat tgcaacgaat accgaagtat ctaaagcatg catggaaggt
      181 ctcaccgagt tctacaggtc ccggggaatc agtgttgtga ccagagtggt cgaaggtttg
      241 acagatgatt cgagtacagc ctttaaaaag gggatcgcag attacgtaga tctgattatc
      301 agcctctttg acaagggcga gcagtggtat tacgagaact tcttcaatgc cacctccgga
      361 tacagatcgc tgatcccgta cacgacgatc gttgcggccg tcttcgattc cagggtattc
      421 tatctctacg agaactcgcc gttcttgctc gatcttccac ccttcccgat caatttcgac
      481 ttcggtataa tggagcaaca ttcagctttc ttcgaaaaag ttgagaacga ttgtgttcct
      541 tccagagagg caatagaaga gtttggatat gaacttttct ccaaagttgt cccgcaagtg
      601 ttgcttgaag aagatgggct ggtctatctc tcgaactttg gaaaaatcct ttggggtagg
      661 tacactgtct cgaaccccga aatctacttc agcgattcag cccaaaaatt tataaacaga
      721 gaccctgttt atctcgacta tgtcagaaag atgctcaggg gagaggccga agccgaacac
      781 tatcttcacg aatatgttgg agatgctcag tgctataaat ttccggcgaa aggggtcagg
      841 atattctaca taagagaggg agatggcccc ttcagaatag cggccatact tcctaaccat
      901 gacgaatacg agagatatat caaaacaaaa caacacgaaa gtagcctgag ctacacaaaa
      961 accagaataa atctctgaag tccgggtaag gagaactgaa gatctagaat tattgaaagc
     1021 ggacacgcca aaggttcctg tgaaacacct gatgcgggca gccattttcc aatctggtta
     1081 tctgctcaaa gccagtcgcc tcaggcggcc agtttgcttt ctaagaacgt tttttagtgg
     1141 tgggttgtgc gctgttgcgc acaacctcca ttatattaag gaacccgctt gccgcttctc
     1201 aaaatcgctt ccccgccagt tcgatcatcc ccattcccag ggagtttttc tggcccagtc
     1261 cgcagtggta tagaaaagct atctcttctt tctttcccgt gattttgacg ggtaccatcg
     1321 agccgaatac ttccgtgtct tctttgatct ttagccttaa gttgcttttt cggccggttc
     1381 tcttcaggta ttccctatcc ac
//
LOCUS       DAOOLY010000427         1376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_126511_length_1376_cov_3.545799, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000427 DAOOLY010000000
VERSION     DAOOLY010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126511_length_1376_cov_3.545799"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLD15_12840"
     CDS             complement(<1..339)
                     /locus_tag="PLD15_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPB64849.1"
                     /translation="MFENLQDKLGRAFKLLSGKGRISEKNIEEAVKQVKLSLLEADVN
                     YKVVKEFINGVTEKSLGEEVLKSFSPDQQFIKIVRDELITMLGERPVPLRLSSQPARI
                     MMVGLQGSGKT"
     gene            complement(572..1147)
                     /locus_tag="PLD15_12845"
     CDS             complement(572..1147)
                     /locus_tag="PLD15_12845"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPB64850.1"
                     /translation="MAGKLTVVVGPMYSGKTSTLLSMVEIYTLGRKNVKVFKPLIDNR
                     YSTDHVVSHTGQKVPAVNVKNSAEVMMAVEAESGRLDAVFVDEVNFFDPELLEVVQQI
                     IFDGIDVFCVGLDMSYKHRPFAITASLMAIADEVVKKKAVCHVCGEYNATVTHRTSNH
                     IDSEIDVGGMDKYIAVCRDCYKKLNSKKQNL"
     gene            complement(1160..>1376)
                     /locus_tag="PLD15_12850"
     CDS             complement(1160..>1376)
                     /locus_tag="PLD15_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64851.1"
                     /translation="KGAIHRGVIHSPPVPGADKFGSGDLFGAVFSSGLLQGLTAEEAL
                     KVAVLESSKYCLFDGSLADYLFQMKKR"
BASE COUNT          332 a          361 c          289 g          394 t
ORIGIN      
        1 cgttttaccg cttccctgga gacccaccat cattatcctg gcgggctgac tcgatagtct
       61 cagcggtacg ggtctttctc caagcatggt tataagttcg tccctgacta tttttatgaa
      121 ctgctggtcc ggagaaaagg acttcaaaac ctcttcgccg agggattttt ccgttacgcc
      181 gttgatgaac tccttcacga ctttgtagtt gacatcggct tccaggagcg aaagcttcac
      241 ctgttttacg gcttcttcta tgtttttctc ggatatcctt ccctttccac ttagcagctt
      301 gaaagctctg ccaagtttat cctggagatt ctcaaacatc caaacacctc cgcagcctga
      361 aaagtatact acgaggtttc cttcgattgt tgaatttaca cgaacatcga aggtgataat
      421 acccgccttt aattaaatta tggttactat aatgaaatca ataaacctaa ctaatctata
      481 aaaactgtgg gatgaagaga gtgtttaatg tggatatcaa aactccgaat tacgtgatca
      541 ccgatgacaa agcatatcgg ctgataccag cttataaatt ttgcttcttt gaattcagtt
      601 ttttgtaaca atctcggcag acggcaatat atttatccat tcctccaacg tcgatttcgc
      661 tatcgatatg gttggaagtt ctgtgcgtga ccgtcgcgtt gtactccccg cagacatggc
      721 agaccgcttt cttcttcaca acctcgtcgg caatggccat aaggctggcc gttatggcga
      781 atggacggtg cttgtagctc atgtccaggc ccacacagaa gacgtctatt ccatcgaaga
      841 ttatctgctg aactacctcc agcagctccg gatcgaaaaa gttcacttca tccacgaata
      901 cggcatcgag cctgccactc tctgcctcca ccgccatcat gacttcggcc gagtttttta
      961 cattcaccgc gggaaccttc tgacctgtgt ggctgacaac gtggtccgtc gagtaccgat
     1021 tatctatgag tggtttgaag accttgacgt ttttccttcc aagtgtgtag atctctacca
     1081 tcgagaggag cgttgaggtc tttcccgagt acatgggtcc cacgacgacc gtaagctttc
     1141 cagccataga cttacctcct catctttttt tcatttgaaa caggtaatca gcgaggcttc
     1201 catcgaaaag acagtatttc gagctttcca gtactgcgac cttcagggct tcttcggcag
     1261 ttagtccctg taacaacccg gaagaaaaca ctgcaccgaa gaggtctccg cttccgaact
     1321 tatccgctcc cggtaccggg ggactgtgaa tcacacccct gtgaatggca cctttg
//
LOCUS       DAOOLY010000428         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_128683_length_1361_cov_5.320827, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000428 DAOOLY010000000
VERSION     DAOOLY010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128683_length_1361_cov_5.320827"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..641)
                     /locus_tag="PLD15_12855"
     CDS             complement(138..641)
                     /locus_tag="PLD15_12855"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPB64852.1"
                     /translation="MTEFKFPEHSYELLKSLKEGRVLLGVTDGVERNLMTVAWGFIGY
                     TWNRPVFIAMVRPSRYTHNFFRKSDVFSVNFMSEQWKDALTFCGTRSGRYFDKFKETG
                     LTPSEGLTTSVMFVREADAVLECRIVSREAITPLALNESIASAFYGDNSYHSLVMGEI
                     TDSYLLP"
     gene            complement(689..1156)
                     /locus_tag="PLD15_12860"
     CDS             complement(689..1156)
                     /locus_tag="PLD15_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPB64853.1"
                     /translation="MLLKGERAVDFSLYGTDLKTYRLSSLFGKKIVLVFYPGAFTSVC
                     QKELCTFRDSLANFERLDAQVIGISVDGPFANRAFKDQNSLSFPLLSDPGGEISRKYG
                     GVHEDFAGVRGLSVAKRAVFIIDREGKIVYSWVSDDPKIEPNYNEISDRLEKI"
     gene            complement(1239..>1361)
                     /gene="ffs"
                     /locus_tag="PLD15_12865"
     ncRNA           complement(1239..>1361)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD15_12865"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          315 a          386 c          322 g          338 t
ORIGIN      
        1 cgctcttctt cgccagagac atactcatga tcgacgggac cggaaggtac agccttcaga
       61 ctccccagtg ggctttatac atactatggt ccatgctggt cttcatgatc gtattcaccc
      121 tgatcgttgc gatcgtttca aggaagaagg tatgagtcgg ttatctctcc cataacgaga
      181 gaatggtacg agttgtcccc gtagaaagcg ctcgcgatgc tttcgttcag tgccagcgga
      241 gttatggcct ctctggagac gatcctgcat tccagaaccg cgtcggcctc cctcacgaac
      301 atcaccgaag ttgtaagacc ttccgagggc gtaagcccgg tctctttgaa cttatcgaag
      361 tatctgccgc tccgggttcc gcagaaggtc agcgcatcct tccactgttc ggacatgaag
      421 ttaacggaaa aaacgtcgct ctttctgaaa aaattgtgcg tgtatctact gggcctcacc
      481 atggcgatga agaccgggcg gttccaggtg tagcctatga agccccaggc gacggtcatc
      541 aggtttcgtt ccaccccgtc ggtgactccg agcagaaccc taccctcttt caaagacttc
      601 agaagctcgt aagagtgttc tggaaatttg aactccgtca taataaacct acctcgcttt
      661 ctttttaaaa caccgggatc gctccccgtc aaatcttttc cagtctgtcg cttatctcgt
      721 tgtaattggg ttctatcttc ggatcgtccg aaacccacga atacactatc ttgccttccc
      781 tgtcgattat gaaaacggcc ctcttggcga ctgacagtcc cctgacgccg gcgaaatctt
      841 cgtgaactcc accgtatttt ctggagatct ctccgccagg gtcggaaagc agcgggaagg
      901 aaagcgagtt ctggtctttg aaagccctgt tggcaaaggg accatcgaca cttatgccga
      961 tcacctgcgc gtccagcctc tcgaagttcg ccagagagtc tctgaacgtg cagagctctt
     1021 tctgacagac actcgtaaag gcaccggggt agaacactag cactatcttt ttgccgaaga
     1081 gagagctgag cctgtaagtc ttcaaatcag taccgtagag tgaaaagtca acggctcttt
     1141 cgcctttgag taacatattc cgcacctcct gaataccgtc tgtatttgtc taggttaatt
     1201 ctagcagatg cggagagtat ttcgcagaaa aagaaacacc atgcatccct cttcgacagt
     1261 accgacctgg ccgtacctac catccagctc agactgagcg cccccacggc acacgagagt
     1321 gcccgcttat ggctgcttcc tcccggacct gaccaggttc g
//
LOCUS       DAOOLY010000429         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_140890_length_1285_cov_3.810569, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000429 DAOOLY010000000
VERSION     DAOOLY010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140890_length_1285_cov_3.810569"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..532)
                     /locus_tag="PLD15_12870"
     CDS             complement(146..532)
                     /locus_tag="PLD15_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HPB64854.1"
                     /translation="MAIQRPDDFDRRSKHLQSLDDEQLDRLFWELAGKIVDPLVELAR
                     THTSPSIERSVLLRMGMDSFQAGAIVSRAFELGLLGKGVGHLVLRYSQSTGTSVEEAA
                     KKLAEGQGWEELMKVFKGRKQNAATE"
     gene            complement(532..>1285)
                     /locus_tag="PLD15_12875"
     CDS             complement(532..>1285)
                     /locus_tag="PLD15_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB64855.1"
                     /translation="QQSRRVIGKEPKSGLATHAGGGNLTGTARGLIKTGAEKTSVIGA
                     SVDLSGLHMASDRDFNRKSFTTGHTGFGIPFAVFPDRSDVPRNAARPLRYMDRYLLVK
                     QGEVFYVTEMLARLEGLQRGPAGNTSLTAAIALARELPEDSTIVVQETEYTGAGKAPS
                     AQLTFARKNGVKVVRGDPLTHDVPGKVIAIPENPSQIKYTELGLDRLRESYIRELIKR
                     QIVPSEKDLDYLAREIKLTRDEAARLLKEVSR"
BASE COUNT          226 a          378 c          325 g          356 t
ORIGIN      
        1 gccgtttttc agaggttcgc tgatctggtt gtagttgaag ccatcgactc gtgtgcccgc
       61 tgggagttgt tttcgccatg cccagccctt tctacgcgga gtgtattttt caaggttttt
      121 cagtatctct tccactctca gtttttcatt cggtggcagc attttgcttc ctccccttga
      181 aaaccttcat tagctcttcc cagccctggc cttcggccag tttcttcgcg gcctcctcta
      241 cgctcgtacc tgtgctctgc gaatatctca gcacaagatg gccgactccc tttccgagaa
      301 gacccagctc aaaggctcgc gaaacaatcg ctccggcttg aaaactgtcc atccccattc
      361 tcaggagcac cgagcgctcg atcgaagggc tcgtgtgtgt tcttgcgagc tcgacgagag
      421 gatcgactat ttttccggca agttcccaga acagtctatc gagctgttcg tcgtcaaggc
      481 tctgtagatg tttacttctc cggtcgaaat catcaggtcg ctgtattgcc attaccgact
      541 tacctccttt aagagccgag ccgcctcatc tctggtgagt ttgatttccc gggcaagata
      601 atccaggtct ttttccgagg ggacaatctg gcgtttgatc agctctctta tgtaagactc
      661 tctgagtcta tctagtccaa gctctgtgta ctttatctgt gaggggttct ccggaattgc
      721 gatcactttg ccgggtacgt catgggtgag gggatcgcct ctaaccactt tcaccccgtt
      781 tttccttgcg aaggtcagct gggccgatgg agcctttccc gcgcccgtgt actccgtttc
      841 ctgaaccacg atggtcgaat cttccggcag ctccctggcc agcgctatcg cagccgtcag
      901 gctggtgttt ccggccggtc cccgctggag accttcgagc cgagccagca tctccgtgac
      961 atagaagacc tctccttgct tgaccaacag atagcgatcc atgtatctta gaggcctagc
     1021 ggcatttctg ggcacgtcgc tcctatcggg gaagacggcg aagggtattc caaatccggt
     1081 gtggccggtg gtgaaggact tcctattgaa gtctctgtcg ctggccatgt ggagaccaga
     1141 cagatcgacg ctcgcaccga tcacactggt cttttcggct ccagtcttaa tcaaccccct
     1201 ggccgttccg gtgaggttac ctccgcctgc gtgggtcgcg agaccggatt tgggctcttt
     1261 accgatcact cgtctcgact gctgt
//
LOCUS       DAOOLY010000430         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_141563_length_1281_cov_4.584013, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000430 DAOOLY010000000
VERSION     DAOOLY010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141563_length_1281_cov_4.584013"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="disA"
                     /locus_tag="PLD15_12880"
     CDS             complement(<1..867)
                     /gene="disA"
                     /locus_tag="PLD15_12880"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPB64856.1"
                     /translation="MERSEFIEKLKIIAPGTPLRHALDDIQDASIGGLLFFVDDYKKY
                     SNLMQIGFLLNCSFNANKLYELAKMDGAIVVTEDLSMIIGANVQLIPDHNISTNQTGM
                     RHRAAERMAKETGKMLVAVSKRRNTMTIFFGDFVYTLNNVETLTSKITQTIRTAEKYK
                     EAFIEALTGMEFMERAGSLTLFDVVKTIQKGLMVMMIGQEAEVTIAELGNHGRFPQMQ
                     ITEMLVNVGEEIENLVLDFSDELVESEEMEETLTRLNGLTEKEINSFGSILRILGYDI
                     TTASQAVEYFIRS"
     gene            complement(854..>1281)
                     /locus_tag="PLD15_12885"
     CDS             complement(854..>1281)
                     /locus_tag="PLD15_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPB64857.1"
                     /translation="IQALVSRPIYGTPRRLSTGVPLERVLLVAAVLTKRANIPLDSLD
                     LYVNVAGGLQIEDTGVDLAVATALISSFTDTAIPDNFAVFGELGLDGTVRSVSSAERR
                     LDRVKNSPFNEVISPEGKNGIKDVKQLLRLIGGIKSGEK"
BASE COUNT          285 a          336 c          305 g          355 t
ORIGIN      
        1 ggaacgtatg aagtactcca ccgcctgaga agccgtagtg atatcgtacc ctaagattct
       61 gagaattgaa ccgaaactgt tgatctcttt ctcggttagt ccattgagtc tggtcagagt
      121 ctcttccatt tcttcagatt cgaccagttc atcgctgaaa tccagcacca ggttctcgat
      181 ctcctcaccc acgttgacca gcatctcagt gatctgcatc tggggaaacc ttccgtggtt
      241 tccaagttcg gcaattgtta cctccgcttc ctggcctatc atcattacca tgagtccttt
      301 ctggattgtt ttcaccacat cgaagagagt gagcgagccg gccctttcca tgaactccat
      361 tcccgtcaga gcctctataa aagcctcttt gtatttttcg gccgttctta tcgtctgggt
      421 tattttagaa gtcagagtct cgacgttgtt caacgtatat acgaagtcgc cgaagaaaat
      481 cgtcattgtg ttccgcctct tggagactgc aacgagcatc ttccccgttt cttttgccat
      541 tctctctgcg gcgcggtgtc tcataccggt ctggttggtg cttatgttgt ggtcaggaat
      601 cagctgtacg ttggcaccta tgatcatcga aagatcttcg gtcacaacga tggcgccgtc
      661 catcttggcc agttcgtata atttgtttgc gttgaaggag cagttcagaa gaaagccgat
      721 ttgcatcaag ttgctgtact tcttgtaatc gtccacgaaa aagagcagcc cgccgatgct
      781 ggcatcctga atgtcgtcga gggcgtgcct gaggggagtt ccaggggcga tgatcttcaa
      841 tttctcaata aactcacttc tctccacttt ttatccctcc gatgagtctc aaaagttgct
      901 tcacgtcttt tataccattc ttgccctcgg gagaaatcac ttcattgaag ggcgaatttt
      961 tcacgcggtc tagtctccgt tccgcagaag acacggagcg aaccgtaccg tccagtccca
     1021 gttcgccgaa aacggcaaaa ttatctggaa ttgcagtatc tgtgaatgaa gatatgagag
     1081 cagtggcgac cgcgaggtcc accccggtat cttctatctg cagaccaccg gcaacgttga
     1141 cataaagatc tagactgtcg agcggtatgt tagccctctt ggtgagaacg gccgccacca
     1201 gaagaactct ctcgagcggt acgccggtgg aaagcctccg cggggtcccg tagataggcc
     1261 tggatacaag ggcctggatc t
//
LOCUS       DAOOLY010000431         1267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_144052_length_1267_cov_4.865512, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000431 DAOOLY010000000
VERSION     DAOOLY010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144052_length_1267_cov_4.865512"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..>1267
                     /locus_tag="PLD15_12890"
     CDS             146..>1267
                     /locus_tag="PLD15_12890"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPB64858.1"
                     /translation="MAGFTFLYFTGIYAIMAFELVLLIMNYRFSKEPSIVVPDVLKDV
                     YTPDLVEKSILYAKARGELHIFSTLVEFAFVTAGIFRLFPLIERIAVSLAGSFVWQGV
                     LFFTILALIGYLSMLPFHVYSTFVLEKRFGFNKATPTTFIVDSLKGILLMAAVGVPLL
                     ALSLMAIEKLAYWWIYLLLGIIIFQLLSGLLYPVLILPLFSKLKPLEDGQLKQAIFDL
                     SKKAGFEVKSIFVMDASRRTGHTNAFFTGFGKARRIVLYDTLIEKHSRSEIEAIFAHE
                     AGHYKYRHTLKGTVLSFAITGLSIFLLWFMVRSGIIETIFAVEREFTALLYSGVFVMS
                     LFSVFGWLGSYISRRWEFAADSYATEVMGTAGPKIDALKR"
BASE COUNT          285 a          328 c          304 g          350 t
ORIGIN      
        1 aaacctcttc acatcttctt ttgagatctt cattcccggc aactcctttc gtgtcatctc
       61 ttctttttga atgttatcat atcgacttca ggatcatcgg gcacccagga aggttttatc
      121 gtagaattta ttacgaggtg agtggatggc aggctttact tttctctact ttacagggat
      181 atacgcaatc atggcttttg agctggtact gctgatcatg aactaccgct tctctaagga
      241 accgtcgatc gtggtacccg atgttttgaa agatgtttac accccggatc tcgttgagaa
      301 atcgatcctc tacgcgaagg cgaggggtga attacatatc ttttcaaccc tggtggagtt
      361 cgcgtttgtc acggccggta tcttccggct ctttccgctg atcgagagga tcgcggtatc
      421 gctggccgga agcttcgtat ggcagggtgt gctcttcttc acaattctcg ccctgatcgg
      481 atacctgtcg atgcttccct tccacgttta ctcgacattc gtgttggaaa aacgcttcgg
      541 attcaacaaa gccacaccta ctacgttcat agtagacagc ttaaagggaa tccttctcat
      601 ggccgcggtc ggcgtcccgc tactggccct ttcacttatg gctatagaga agctcgctta
      661 ctggtggatc tacctcctat tgggtatcat catcttccag ctcctgtcgg ggttgctcta
      721 tccggtactg attctacctc tcttctcgaa gctgaaaccc ctggaggacg gacaactcaa
      781 gcaggctatc ttcgatctat cgaagaaggc cggtttcgag gtcaaatcca tcttcgtcat
      841 ggacgcctcc agaaggaccg gacacaccaa cgcttttttt acagggtttg gcaaagccag
      901 gagaatagtt ctctacgaca cccttataga gaaacactcc cggagcgaga tagaggcgat
      961 cttcgcccac gaagccggcc actacaaata cagacacacg ctcaaaggaa cagtgctatc
     1021 ttttgcgatt accggactat cgatattcct actctggttc atggtaagat cggggatcat
     1081 agagacgatc tttgcggttg aaagagaatt caccgcactg ctgtattcgg gagttttcgt
     1141 catgtcgctc ttctcggtct ttggatggct cggctcttat ataagccgca ggtgggaatt
     1201 cgccgcagac agctatgcga ctgaagtcat gggcaccgcc gggccgaaga tagacgccct
     1261 gaagagg
//
LOCUS       DAOOLY010000432         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_146753_length_1253_cov_2.083472, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000432 DAOOLY010000000
VERSION     DAOOLY010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146753_length_1253_cov_2.083472"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..492)
                     /locus_tag="PLD15_12895"
     CDS             complement(7..492)
                     /locus_tag="PLD15_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64859.1"
                     /translation="MTSTKILSIALIVFLAGVGLSASISGALLKLTEIAGLEKKIAGD
                     LEKSLTTINRFYCGGISYGDSLVGVSFSFFTGEEQPLTRDGIKDTTRDLASRSVAIPF
                     LRQLSRVQSERNNKPLLSALLFEKPAYFSLQYRAHTSGFQRRMGWYDRDREAKQSRYP
                     A"
     gene            complement(479..>1253)
                     /locus_tag="PLD15_12900"
     CDS             complement(479..>1253)
                     /locus_tag="PLD15_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64860.1"
                     /translation="KSGKVYVSIVISPKTLAAVNQVSDGVISSADLATSLQFILNDIE
                     AGIVPPVGGKVIYVPDTGTTAVVAFGSAIARHSSNEVLANEYKKAAIETARMRATQTL
                     VDILNGNINIWKSGFEGKTLRAEFENMDEVITKADELRNTRSDSVAETISSEFLNLFS
                     RDKNYESVSSGIVPAGTLIRIAMEESVEKNGYGWVFAIAVYYPELELPMQQIIIAMEG
                     KPIVTEQPVKETTTEEKKESGTSENPQGPTGPVSSDDKY"
BASE COUNT          315 a          302 c          267 g          369 t
ORIGIN      
        1 agaccatcag gctggatagc gagattgttt tgcttcacga tcacgatcgt accaacccat
       61 tcgtcgctgg aagccagacg tatgagctct gtactggaga ctgaaataag caggtttttc
      121 aaagagtaag gcagaaagta gaggtttatt atttcgctca gattgaaccc tggaaagctg
      181 tcttaaaaag ggaattgcaa ccgatctgga agcgagatcc cgggttgtgt cttttattcc
      241 gtctctggtg agcggctgtt cttctcctgt gaaaaatgag aaagagactc ccacaaggga
      301 gtctccgtaa gatattccgc cacagtaaaa gcgatttata gtggtcaggc tcttttccag
      361 atcgccagcg attttctttt cgaggcctgc aatttcggtt aacttcagta aggctcccga
      421 aatcgaagcc gataatccca ccccggccag gaatactatc aaggcaatag aaagaatttt
      481 agtacttgtc atcactgctc accggaccgg ttggaccttg agggttctcg ctcgttccac
      541 tctctttctt ttcctctgtc gtggtttctt ttaccggctg ttcagtaaca attggttttc
      601 cttccatagc tatgattatt tgctgcatag gcagttctag ctccgggtag taaacagcta
      661 tagcaaatac ccatccataa ccgttcttct caaccgactc ttccatagct atccttatca
      721 gagttcccgc cggtacaata ccagatgaaa ctgactcgta gttcttatct ctactgaaca
      781 agtttagaaa ctcgcttgat atggtctcag caacgctgtc ggatcttgta ttccttagtt
      841 catcagcttt agtaattact tcatccatgt tttcaaactc tgcccggagc gtctttcctt
      901 caaatccgct cttccagata tttatatttc cgttcaagat atctaccaga gtttgtgtag
      961 ctctcattct tgcagtttcg attgcggctt ttttgtattc gttggctaaa acttcgttgg
     1021 aactgtgtcg agcaatcgcc gaaccgaaag cgactacagc cgttgtaccg gtatcaggaa
     1081 catagatcac ctttccaccg accggaggaa ctatgccggc ttcaatatcg ttgaggatga
     1141 actggaggga agtcgcaaga tccgcagaag aaatcactcc atctgaaacc tggttaacag
     1201 cggcgagtgt cttcggagaa ataacgatgc taacatagac ttttccactc ttc
//
LOCUS       DAOOLY010000433         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_148787_length_1241_cov_3.556492, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000433 DAOOLY010000000
VERSION     DAOOLY010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148787_length_1241_cov_3.556492"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLD15_12905"
     CDS             <1..558
                     /locus_tag="PLD15_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB64861.1"
                     /translation="RFKGRGTVQRSVLFVYLFGGTVIMVPLYQMAVKLGILSSPLGTS
                     IYLIMVYVIQTLPVSLYMLGNYFRTIPYSIEEAAIIDGCSRVQAIFRIVIPLSLPAIA
                     TVYIYAFMIGWNEYLFASAFLGLKSYKDLFTLPIGLSTFSGSAHAVWGRLMAASVVSA
                     IPIIIIFSLMQKYLTSGFTAGGVKE"
     gene            580..>1241
                     /locus_tag="PLD15_12910"
     CDS             580..>1241
                     /locus_tag="PLD15_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB64862.1"
                     /translation="MSGLKHDSYDIVVGIPSFNNRSTIKHVVEVSSRGLKEYFPELKS
                     CIVNSDGGSGDGTEGSFYSANSMGLDLLSFKYTGISGKGSAMRSILELASRLGAKAVV
                     FLDSDLRSVEPFWIERLAGPIINLDASYVAPYYIRHKYDGTITNSICYPLTTSLYGRK
                     VRQPIGGDFGVGREMIDYYVSMPVEAWNCDISRFGIDIWMTTSAINEARGKIYQAALG
                     SK"
BASE COUNT          297 a          296 c          319 g          329 t
ORIGIN      
        1 cgcttcaaag ggagaggaac ggtacagaga tcggtgctgt ttgtgtatct gttcggaggc
       61 accgtgataa tggtcccgct ttatcagatg gccgtaaagc tgggaatcct ctcctcaccc
      121 ctgggtacga gcatctatct gattatggtt tacgttatcc agactctacc ggtttctttg
      181 tatatgctgg gtaattactt cagaaccata ccctattcga tagaagaagc ggctattatc
      241 gatggctgtt cgcgtgttca ggcgatcttc agaatcgtta tacccctttc tctgccggcg
      301 atcgccactg tatatatata cgcattcatg ataggctgga acgaatacct tttcgcttcg
      361 gcctttctcg gcttgaagtc ttacaaagat ctcttcacac tgcctatagg ccttagcact
      421 ttctccggtt cggcacatgc ggtatgggga agactgatgg cggcctctgt ggtctcggca
      481 atccccataa taattatatt cagcctcatg cagaagtatc tcacatccgg tttcaccgcc
      541 ggaggagtca aagaataaaa cggaatggga ggttctattt tgtccggttt gaagcatgat
      601 tcatacgata ttgtggtggg catacccagt ttcaacaacc gatccacgat aaaacacgtc
      661 gtcgaggttt cttccagggg actcaaggag tatttccccg aattgaaaag ctgcatagtg
      721 aattccgacg ggggctccgg tgacggaacg gagggatctt tctattcggc caactctatg
      781 ggcctagatc tcctgtcgtt caagtacaca ggaatatccg gcaagggaag cgcgatgaga
      841 tctatcctgg agctcgcgag ccgtctgggc gccaaagccg tggtttttct cgattccgac
      901 ctcaggagtg tcgagccttt ctggatagag agactggccg ggccaattat taatctggat
      961 gcttcatacg tggcccctta ttatatccgc cataaatacg acggaaccat caccaacagt
     1021 atctgctatc ccttaacaac gtcgctttac gggagaaaag tgcgccagcc tatcggtggc
     1081 gacttcggtg tgggcagaga gatgatagac tattatgtgt cgatgccggt agaagcctgg
     1141 aactgtgata tctccaggtt tggtatcgat atctggatga cgacttccgc gataaacgaa
     1201 gccaggggaa agatatacca ggctgctctg gggagcaaga t
//
LOCUS       DAOOLY010000434         1238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_149357_length_1238_cov_3.451395, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000434 DAOOLY010000000
VERSION     DAOOLY010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149357_length_1238_cov_3.451395"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLD15_12915"
     CDS             <1..583
                     /locus_tag="PLD15_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HPB64863.1"
                     /translation="LLRYRNSCYSCKMFGSTAVGSIVKFDDLFPYRPENNSEEKMRAV
                     TEIEKYLNIRHGIKIDRKSGSVARSALYDIECLSGGKFYGSIFLKNPEKWQLALLLKT
                     FTHINHGYQKAGGQKSRGFGHVNLEIEKISCLSKLRDKIVFSSFDRARGFIDDPMSVI
                     QSSDVFPPYSFEFSGEALEKFEVDVNSTLSRAW"
     gene            589..960
                     /locus_tag="PLD15_12920"
     CDS             589..960
                     /locus_tag="PLD15_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64864.1"
                     /translation="MNDRLEHLERLKSTEGLFYVIFNDYRALQLDKMTPDESIELFNK
                     LHDSFDYNFYFRAFNEYLDFVWFYGDIYVSDRKNADERVVYLNAGIRKKIPFSNPVIK
                     FVSDLSMPGGMRYIRVEEEGA"
     gene            960..>1238
                     /locus_tag="PLD15_12925"
     CDS             960..>1238
                     /locus_tag="PLD15_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64865.1"
                     /translation="MPDYNDSPKGYMRLVIPKNTSHIESRDFSHIDSLRDDRLNGVLK
                     LEVVTRSNTCFTQGDLLMKEGKITNVLSREGTRVLIPGASLKGAIRTYA"
BASE COUNT          376 a          232 c          290 g          340 t
ORIGIN      
        1 tctgctaaga taccggaatt cctgttattc atgcaaaatg ttcggctcaa ctgccgttgg
       61 atcgatagtc aaattcgatg atctctttcc ttataggcct gagaataatt cagaagaaaa
      121 gatgagagcg gttaccgaaa tcgagaagta tcttaatata aggcatggaa taaaaatcga
      181 caggaaatct ggttccgttg ccaggagcgc tctttatgat attgagtgcc tctctggtgg
      241 caagttctat ggaagcatat tccttaagaa tccagaaaag tggcagctcg ctctgttgct
      301 caagactttt acacatataa atcatggtta tcaaaaagcg ggtgggcaga agtcgagagg
      361 ttttggccat gttaatctgg aaatcgagaa gatatcctgt ctttcgaagt tgagggataa
      421 aatcgtcttc tcttcatttg acagagccag aggatttata gacgacccta tgagtgtaat
      481 tcaatcttcg gatgtatttc caccgtacag ctttgagttt tcaggcgagg cattggagaa
      541 atttgaagta gacgtcaact ccactctttc aagagcgtgg tgagacagtt gaacgacaga
      601 cttgagcatc tcgagagatt aaaaagcact gaaggattgt tttatgtgat ctttaacgac
      661 tacagggctc ttcaactcga caaaatgacc cccgatgaaa gtatagaact attcaataaa
      721 ctgcatgata gtttcgatta taatttctac ttcagagctt tcaacgagta tctggatttt
      781 gtgtggttct atggagatat ctatgtcagc gacagaaaga acgccgatga aagggtagtt
      841 tatctcaatg ccggtatcag gaagaagata ccattttcta atcccgtaat aaagtttgtc
      901 agtgaccttt cgatgcctgg cggtatgagg tatatcagag tagaggagga aggagcctga
      961 tgccagatta caatgactct ccaaagggat acatgaggct cgtaattccc aaaaacactt
     1021 cacatattga atcgagagat ttctctcaca tcgattcact tagagatgat cggttgaacg
     1081 gagtattgaa gctcgaagtg gtaacccggt caaatacatg tttcactcag ggagatctcc
     1141 ttatgaaaga ggggaaaatc actaatgtcc tgtcgagaga agggacaaga gtactgattc
     1201 ccggggcatc actcaaaggg gccatacgca catatgcg
//
LOCUS       DAOOLY010000435         1234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_150104_length_1234_cov_4.578456, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000435 DAOOLY010000000
VERSION     DAOOLY010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150104_length_1234_cov_4.578456"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLD15_12930"
     CDS             complement(<1..280)
                     /locus_tag="PLD15_12930"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPB64866.1"
                     /translation="MGERIQANLENWLLTAPDANPGMIEMFRIRDRKPKPELVPWAGE
                     FVGKYLISAIQAMRMTDDPRLPILLKRVVAELVATQADDGYLGPFRKEE"
BASE COUNT          332 a          284 c          340 g          278 t
ORIGIN      
        1 gttcctcttt gcggaacggg ccgaggtagc cgtcatccgc ctgtgtcgct acaagttccg
       61 cgacaacacg cttgagaaga atcggcagcc tgggatcgtc ggtcatccgc atggcctgga
      121 tagccgagat caggtatttg ccgacaaatt ctccagccca cgggaccagt tccggtttcg
      181 gtttcctgtc gcggatgcga aacatctcga tcatgccggg attggcatcc ggcgcggtaa
      241 gaagccagtt ctcaagattg gcctggatgc gttctcccat cggcccaccg aattgaagcc
      301 ggatatccgt tggttgatga aaaaccagtt tgcccgattc acgttccccc tgcgctacct
      361 cggctgcgtt ggagggtaga ttccccatca aaaacaatag cgcgattgca gataagcaag
      421 cgaatcggaa ggggagagag ggtagggggg acatgggaga cctttctggg gaagtggatt
      481 cacaccttgg gaatgatagg acagcggtaa gccggagtca attaggaggg ggtggtatga
      541 gcggcgggtt ccggcaatac gtcggctcag actcacggaa ctatgatggt gttgacggaa
      601 tgagttatca ggtactggag tcactaaaca cgaattcacc tcggctgtcg gcaaatgaca
      661 gccacgcgtc aaggccatcg cattcaagtt ccacgcatga cagagatggt cgaagaagtg
      721 ctgtcgctct agtcgataaa agaaagacat ttgtgaagtc ttgaagaaca gcatgatttt
      781 cgccggtttg aaagaaggct aagtagaaag tgaatgagcg cagatgaacg gatctcaaca
      841 acttctgacc aatcccatac gtgcggaaaa gcaggtgtcc aactcgattt gctgttgggc
      901 gaaagaatac cggacgatac ttcggagcct catcggttag catatcttcg ggtatctcgc
      961 ctggatagga gagctgtagg aaaagaagga cagatcgccc cactttgatg tcttttccca
     1021 taatataagg taataatggc tcaaacacaa acagatcgcg cgaggaattc acacgggcag
     1081 agttatagca tgtttttagg agccatcgtt ttagaaagtg atagtcgaac tcaaagacca
     1141 ctctttcgtg acgatctaga attcgaacaa agtatcggtc gaacagaaag catatatacc
     1201 tatctaattc cgacagtacg gtgttattac acct
//
LOCUS       DAOOLY010000436         1225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_152017_length_1225_cov_2.335043, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000436 DAOOLY010000000
VERSION     DAOOLY010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152017_length_1225_cov_2.335043"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLD15_12935"
     CDS             <1..751
                     /locus_tag="PLD15_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPB64867.1"
                     /translation="KIGKKPEDIYLVSIMPCTAKKDEKSRETLSVRGMQTTDAVIVTR
                     ELAKLIKMYKIPYESLEEEPYDIPLGISTGAAAIFGTTGGVMEAALRTAYELYTGEEL
                     PKLDFEFARGFDGVKEAEIDMKGTKVKVAVVHGGSNVVQLMDKIRSGEKFYHFVEVMA
                     CPGGCIGGGGQPKSSKPGYLQKRMEAIYNIDRDSKIRKSHENPAITKIYEEYLGKPLG
                     HLSHELLHTHYKNNQKSVIERKKELEEVSKG"
     gene            complement(796..>1225)
                     /locus_tag="PLD15_12940"
     CDS             complement(796..>1225)
                     /locus_tag="PLD15_12940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB64868.1"
                     /translation="VGKNGLLFKMYKFRTYGTRDGRNEITILGRVLRPLRLDELPQIV
                     NIIKGDMSLVGPRPELPSFHRLGIDNIPDYSLRLLVRPGLTGWAQINYKYTVTVEEYR
                     IKTAYDLYYVARRSFCLDLKCLLKTPYALAVTVFERENDQ"
BASE COUNT          357 a          263 c          298 g          307 t
ORIGIN      
        1 gaagatcggc aagaaacccg aagatatcta tctggtctcg ataatgccct gtacagcaaa
       61 aaaagatgaa aagagtagag aaactctttc ggtaaggggc atgcagacaa ccgacgctgt
      121 tattgtaacc agagagctgg ctaaattgat caagatgtac aagataccct acgaatcgct
      181 cgaagaggaa ccttatgata tacccctggg aatctccaca ggagccgccg cgattttcgg
      241 aacaaccgga ggagttatgg aggctgccct tagaacggct tatgagttgt acaccggtga
      301 ggaacttcct aaactcgatt tcgaatttgc cagaggtttc gacggagtta aagaggccga
      361 gatcgatatg aaaggtacaa aagtgaaagt tgccgtagtt cacggcggta gcaacgtcgt
      421 acagttaatg gataaaatac gctcgggaga gaaattctac cactttgtcg aagtcatggc
      481 ctgtcccggt ggttgtatag gcggaggtgg ccagccaaag tcgagcaagc ccggttatct
      541 tcagaagagg atggaggcaa tctacaacat cgatagagac tctaagatca gaaaatctca
      601 cgaaaacccc gccataacca agatatatga agagtatctt ggcaaacctc taggtcatct
      661 ctctcacgaa ctcctccata ctcattataa gaataaccag aagagtgtaa tagagagaaa
      721 gaaggaactg gaagaagtat ccaagggttg agagatttat tcgatataat aggggagccg
      781 gtgaggctcc tctttttatt ggtcgttttc tctttcgaaa acagtcactg ccagtgcata
      841 gggagtcttc aagaggcatt tcaagtcgag acagaaagat cttctagcca cgtagtaaag
      901 atcgtaggcc gttttgattc tgtattcctc gaccgttacg gtgtatttgt agttgatctg
      961 cgcccagcca gtcaatcccg gtctcactaa caaccttaga gagtagtctg gtatattgtc
     1021 gattcccagt ctgtgaaagg atggcagttc aggtctggga ccgactagac tcatatcacc
     1081 tttgattata ttcactatct ggggtagttc atctagccga agcggtctta acacgcgccc
     1141 cagaattgtg atttcgtttc gaccgtctct ggtaccgtaa gtcctgaatt tgtacatctt
     1201 gaaaagtaac ccgttttttc cgacc
//
LOCUS       DAOOLY010000437         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_152503_length_1222_cov_4.286204, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000437 DAOOLY010000000
VERSION     DAOOLY010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152503_length_1222_cov_4.286204"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLD15_12945"
     CDS             <1..795
                     /locus_tag="PLD15_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPB64869.1"
                     /translation="GGEWFSQYGVEGSKGTKVFALAGNVKNTGLVEVPMGITVRKLIY
                     DIGGGIPGGKRLKSIQTGGPSGGCIPLEHLDTPITYDNLKKLGTIVGSGGMVVMDEDS
                     CMVDVAKYFLEFTVEESCGQCTPCRDGTRRMLEILERITEGEGTMEDLDLLKELGENI
                     MATSLCGLGQTAPQPVLSTMRYFWHEYEAHVLEKKCPAKRCKPLMRVVIDKDKCVGCT
                     ACARVCPVNAISGAVRKPHEIDPEICTRCGSCLAVCRFNAISKVSP"
     gene            865..>1222
                     /locus_tag="PLD15_12950"
     CDS             865..>1222
                     /locus_tag="PLD15_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPB64870.1"
                     /translation="MSADSIRETVTKIYEDINSQSLEKGDVLINTLHAIQDHYGNFIP
                     IEAAEVLKELTGTPLSKIYEVLTFYTMFSTHRRGKYVIRICKSLPCHVTGGQEVVDAL
                     KNSLEISFGETTGDGLF"
BASE COUNT          335 a          279 c          333 g          275 t
ORIGIN      
        1 ggcggcgagt ggttcagcca gtacggcgtg gaaggttcaa aaggtacgaa ggtcttcgct
       61 ctggccggaa acgtgaagaa taccggtctg gtggaagttc ccatgggaat aaccgtgaga
      121 aagcttatct atgatatagg tggaggtatt cccggtggaa agaggttgaa gtccattcaa
      181 accggtggac ccagcggcgg atgtatcccg ctggaacatc tcgatactcc gattacctac
      241 gataacctca agaagctcgg aacgatagtc ggttccggcg gaatggtagt catggacgaa
      301 gacagctgta tggttgatgt ggccaaatac ttcctcgaat tcaccgtaga agagtcgtgc
      361 ggacaatgca caccatgtcg cgatggtacc aggagaatgc tcgagatcct cgagagaatt
      421 accgagggcg aaggtaccat ggaagatctc gaccttctca aggaactagg cgagaatata
      481 atggcgacat ctctctgtgg acttggccag acggcacctc aaccggttct ttcaacgatg
      541 agatacttct ggcatgaata cgaagcgcac gttctggaaa agaaatgccc cgcaaagaga
      601 tgcaaacctc tcatgagagt cgtgatagat aaagacaaat gtgtcggctg cacggcctgt
      661 gccagagtct gtccagtgaa cgcgatttct ggcgcagtca gaaagcccca cgagatagat
      721 cccgaaatct gcactcgctg tggcagttgt cttgcggtat gcagatttaa cgcaatcagc
      781 aaagtctctc cgtgagatat ttgagaaccc cctttcgagg gggttcttct gcattgtcaa
      841 tttgggttga agggaggaat tagcatgtct gctgacagta tccgcgagac ggttacgaag
      901 atttacgaag atataaatag tcagagtctc gaaaagggcg acgtactgat aaacactctg
      961 cacgctattc aggatcacta cggcaatttc attccaatcg aggcggctga ggtgttgaaa
     1021 gagctgaccg gtacacccct gtcgaaaata tacgaagtct tgactttcta caccatgttc
     1081 tccactcaca gacgcggaaa gtacgtaata aggatctgta agagccttcc ctgccatgta
     1141 accggcggac aggaagtcgt tgatgcgctt aagaacagcc ttgaaatcag tttcggtgag
     1201 acaaccggcg acggtctatt ca
//
LOCUS       DAOOLY010000438         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_153100_length_1219_cov_4.546392, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000438 DAOOLY010000000
VERSION     DAOOLY010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153100_length_1219_cov_4.546392"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..682)
                     /locus_tag="PLD15_12955"
     CDS             complement(293..682)
                     /locus_tag="PLD15_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64871.1"
                     /translation="MSRLTRLGYILALVGVTAAIAMLIVMTDFTPYSSETVYPRDSGR
                     TISTFDLSPTQELSIRFDVKIMKSNSAYLVLEKDGVEVFRYSDNWKTIGKEARVSLDG
                     VHEMKVYFTDPAADRRTAEIEIDWIVR"
     gene            complement(805..>1219)
                     /locus_tag="PLD15_12960"
     CDS             complement(805..>1219)
                     /locus_tag="PLD15_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPB64872.1"
                     /translation="DVDEDQLGAEHVAEELGISADMTMKTLVVKGDRSGVFVCCLSGD
                     REIDMKLLESITGDRNIETVPASRLLSLTGYVRGGVSPLGMKKSYRILIDSHALSKER
                     VSMSAGKRGIQIWIKPTDVVKATNGEVKSFSREKH"
BASE COUNT          281 a          333 c          270 g          335 t
ORIGIN      
        1 gtccttacgc ggttgacgaa tgcagggaga aggaagagcc taagctggaa gagctagaga
       61 acggacacta ccacgcctgc agaaggtgga aggaagtcca ggagacggcg acttgagaaa
      121 gaaaagcgca caggcccggg gaacctgatg ggtaataatt cataggtccc tcaatattat
      181 tatagacaca ccggagacgc tggtttcgtc aacggtgtgt ctatattttt caattcagat
      241 cgtctttgaa ctctgttcta tttgctctgt aatagagaga tacgacctct cttcatctca
      301 cgatccagtc gatctcgatc tctgccgtgc gcctgtcggc ggccggatcg gtgaaataca
      361 ctttcatctc gtgtacacca tccagagaga ctcgcgcctc ttttccgata gttttccagt
      421 tgtcggagta cctgaagact tctaccccgt ccttttccag aacgaggtaa gcgctgttgc
      481 ttttcatgat tttcacgtcg aaccggatcg agagttcctg tgtgggcgac agatcgaacg
      541 tcgagatcgt tcttcccgaa tccctggggt acaccgtttc gctgctgtag ggagtgaaat
      601 cggtcatgac tatcagcatc gctatcgccg ccgttactcc caccagagca agtatataac
      661 ccaatctggt caatctagac aagatatttc tccttcactt ttttatccac gctcgttcat
      721 tttctccaat aaccagaggt cagaccgttc acgttcttgg actaataacc aggaacggct
      781 actgacaatc atctaccctc tccgtcaatg cttttctcgc gagaagcttt tcacttctcc
      841 gttggtggcc ttaacgacat ccgtcggttt gatccagatc tgtattcccc tctttccggc
      901 actcatcgaa actctctctt ttgatagagc gtgggagtct atgagtattc tgtaactctt
      961 cttcattccc aagggtgaga caccgccacg aacgtaaccg gtcagggaaa gcagcctgct
     1021 ggccggaacc gtttctatat ttctatcacc cgttatcgat tccagtaatt tcatatcgat
     1081 ctcgcggtcg ccgctaagac aacatacaaa cacgccgctt ctgtcaccct ttacaaccag
     1141 cgtcttcatg gtcatgtcgg cgcttatccc cagttcttcg gctacgtgtt cggcacctag
     1201 ttgatcttca tccacatcg
//
LOCUS       DAOOLY010000439         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_155556_length_1207_cov_3.235243, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000439 DAOOLY010000000
VERSION     DAOOLY010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155556_length_1207_cov_3.235243"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..>1207)
                     /locus_tag="PLD15_12965"
     CDS             complement(310..>1207)
                     /locus_tag="PLD15_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPB64873.1"
                     /translation="ESRPGLIYNLGFDKFFAREDLHDPNQFVGYLGADEFAMLDPIAS
                     WIASENKPFLATILCSVTHDPYEVPAWFGPPADTQTERYLQTITYTDRFLAALDAKLS
                     ELGVAKDTIFCVVGDHGEAFSEHQMMGHERIYYDEVLRIAMCIRAPFMVNPGRRVTHP
                     VSSVDFTPTILSLLGFEIRPMNFDGDDALGSLPSDRRVYLSGWMQQGPAGFVQGNMKF
                     VYDPENGRVRIFSLTTDPLELSGMDLPAAEAETLSKQITDWRKATILSVDEDARGKKN
                     VFDSWQSRWAGRRSKVKYIAND"
BASE COUNT          269 a          308 c          377 g          253 t
ORIGIN      
        1 caaagagccg gttgacaagt ccaccgtttc atgggcgtta catgcgacac tataggacgt
       61 gccggattct gagagatcga acgctctcgt agttttacga cttcaccgtc gcatggcaag
      121 aaagaaatgc ccatatatcc cagaatacca cactatcgta aagaccgctt actggaagcc
      181 ggagtccgcg ttattagtac caataaatag gttttgtcat acacgtaggt ttggacaaga
      241 cgcccaatcg gagattgaca gggtacatcg gagcgatgga ggcggttgag ccaggcggac
      301 ggagtggggt cagtcgttgg cgatatactt caccttggat cgacggcccg cccacctgct
      361 ttgccaggag tcgaagacgt tcttcttgcc tcgggcatcc tcatcaacac tgaggatggt
      421 ggcttttcgc cagtcggtga tctgtttgct gagtgtttca gcctccgctg cgggcagatc
      481 catgccggac aactcgaggg gatcggtggt cagggagaag atcctgactc gaccgttctc
      541 gggatcgtag acgaacttca tgttgccttg gacgaaacca gcgggcccct gctgcatcca
      601 gccggagaga tagactctcc gatcggaagg cagcgaaccc agtgcgtcgt ctccatcgaa
      661 gttcattggg cggatctcga agcccagcag gctcaggatc gtgggcgtga agtccaccga
      721 gctgaccggg tgggtcaccc gccggccagg gttgaccatg aatggcgcgc gaatgcacat
      781 ggcgatgcgg agaacttcgt catagtaaat ccgctcgtgg cccatcattt gatgctcgga
      841 gaaggcctcg ccgtgatcgc cgacgacaca gaagatggtg tccttggcca cgccaagctc
      901 ggagagtttt gcatcaaggg cggcgaggaa gcggtcggtg taggtgatcg tctggaggta
      961 acgctcagtc tgggtatcgg cgggcgggcc gaaccaggcc ggtacctcat agggatcgtg
     1021 tgtaacagag cagaggatgg ttgcgagaaa cggtttattc tcggacgcga tccatgatgc
     1081 gataggatcg agcatagcga attcgtcggc tccgaggtag ccgacgaact ggtttggatc
     1141 gtgtaagtcc tcgcgggcga agaacttatc gaaccccaga ttatagatca ggccggggcg
     1201 cgattcg
//
LOCUS       DAOOLY010000440         1196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_157762_length_1196_cov_4.697634, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000440 DAOOLY010000000
VERSION     DAOOLY010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157762_length_1196_cov_4.697634"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLD15_12970"
     CDS             <1..845
                     /locus_tag="PLD15_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64874.1"
                     /translation="VALLSTYFMTMNGGYYPGTSFGTLEKPGISDFWLNPEVFKDLSR
                     FNSDNTTAMAMPLQFGGQTLQIVRIETTGVDTKHVHAYDTASGLLIYSLIQAPSGADI
                     HQSYMEFISARYIDLPWANSPRPSWSMAESLYGGTYTINIPGSYTTPSQMQVNVTPVK
                     SSQSWDLIKMTISQYGILPSEVTTVTGIAQISGAFWLCPQALKSLNRVQTIDQDPVTG
                     VVAEVAFAGKLQDGTNAIMLQMSNGTYMNQYLYDQSTGRLIYIKLQSPNSLSYNVTEL
                     SLIR"
     gene            complement(846..>1196)
                     /locus_tag="PLD15_12975"
     CDS             complement(846..>1196)
                     /locus_tag="PLD15_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64875.1"
                     /translation="DNMARTEFISGLMRSVANGDMETMVNSALFSQIISNVPSAILFS
                     SFTDSYKGLTLGVNIGGSGTIIASLANLIAYRIYVKERGQNGKYLIIFMVSSTVSLLL
                     SLTFGFFRLKFLGL"
BASE COUNT          298 a          339 c          300 g          259 t
ORIGIN      
        1 cggtggcact cctgagcact tacttcatga ctatgaacgg aggctactac ccgggaacct
       61 ctttcggcac acttgaaaaa cccggaatat ccgatttctg gcttaatccc gaggttttca
      121 aagacctgtc ccgcttcaac agcgataaca ctacggcgat ggcaatgccc ctccagttcg
      181 gcggacagac gcttcagata gtgagaatcg aaactaccgg ggtcgacacg aagcatgttc
      241 acgcttacga cacggcgtcc gggttgctta tttattcgtt gattcaggct ccctcgggag
      301 ccgacatcca tcagtcgtac atggagttca tctcggcaag atacatagat ctcccctggg
      361 ccaactctcc cagaccttcc tggagcatgg ccgaaagcct ctacggaggg acgtacacga
      421 taaacatccc cggttcctac accacgccca gtcagatgca ggtgaacgtc actcccgtaa
      481 aatcctccca gagctgggat ttaatcaaga tgacaatatc gcagtacggt atcctgccct
      541 cggaggtgac caccgtcacc gggatcgccc agatcagcgg tgctttctgg ctctgccctc
      601 aggccctgaa atcgctgaac agggttcaga cgatagacca ggaccccgtt accggtgtcg
      661 tcgccgaagt ggccttcgcc ggaaagcttc aggacggtac gaacgcgata atgcttcaga
      721 tgagcaacgg cacctatatg aaccagtatc tttacgatca aagtaccggc aggcttatct
      781 atatcaagct acagtcaccg aacagcctga gttacaacgt taccgaactc tcgctgatca
      841 ggtgatcaca gtcccaggaa tttcagccgg aagaagccga aagtcagact cagaaggagc
      901 gaaacggtgc tggataccat gaaaatgatc aggtatttgc cgttctgccc ccgttccttc
      961 acgtagatcc tgtaagcgat caggttggcg agcgaggcga ttatcgttcc gctgccgccg
     1021 atgttgacgc ccagagtaag gcccttatag ctgtcggtga aagaagagaa gagtatggcc
     1081 gagggaacgt tgcttattat ctgcgagaag agcgccgaat tcaccatcgt ctccatatcc
     1141 ccattggcaa ccgatctcat aaggccggat atgaactcgg tcctggccat gttgtc
//
LOCUS       DAOOLY010000441         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_158451_length_1193_cov_3.077329, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000441 DAOOLY010000000
VERSION     DAOOLY010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158451_length_1193_cov_3.077329"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1193)
                     /locus_tag="PLD15_12980"
     CDS             complement(66..>1193)
                     /locus_tag="PLD15_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPB64876.1"
                     /translation="TPFSKHIGISKKIADPQERHRLTTVAKSLLESKEGVIFRTASYG
                     LGEETIKDELEGLRENWKKINETFKRGRKPKILYEEPSFVEYILRERLTESTKEIVVD
                     SEELWHEVVAGISKFKAGFKPVVRYVEGDAFATEDIYEKLKILYTRTVALPGGGNIYI
                     DRTEAMTVIDVNSAGNLSGSDVSETSLNTNLEAASEIARQLRLRNIGGIVVIDFIDMK
                     TDEDRQKIIASLSSEFKKDKARTMIMGFTRLGLLEMTRKRSTAAIGSKLYSSCPVCKG
                     TGRIESPRAVYQRLLKDLTRGFQDQTINGASVQVYQNMSGILTPETQKNLLKTLKKEV
                     SVGFTWPVPTTYDIKFIKKPEQPKKSKKPEKKKEKEKKESA"
BASE COUNT          234 a          312 c          277 g          370 t
ORIGIN      
        1 gcgcttcctt cacacggaag aggccacagg ttcaatccct gtcgcatcca ccagcgagga
       61 aggggtcagg ccgactcctt cttttccttt tcttttttct tctctggctt tttggacttt
      121 tttggctgtt ccggcttttt gataaacttg atatcgtacg tcgtgggaac cggccatgtg
      181 aaacctaccg acacttcttt cttgagagtt ttcaggagat tcttctgggt ttccggggtg
      241 agtatcccag acatattctg ataaacctgc accgaagctc catttatcgt ctgatcctgg
      301 aaaccgcgcg tcagatcttt gagcagcctc tgataaacgg cccgcggcga ttctatcctt
      361 cccgttcctt tgcagacagg gcaggaagag taaagtttgg aaccgatcgc ggcggtagat
      421 ctttttctgg tcatctcgag aagaccgagt ctggtgaaac ccatgatcat ggtacgggcc
      481 ttgtctttct tgaattcgct gctcagactg gctatgatct tctgcctgtc ttcatcggtc
      541 ttcatatcta taaaatcgat taccacgata ccaccgatat ttctcagccg gagctgtctg
      601 gcaatttccg acgccgcttc caggttggtg ttcagagagg tctccgagac atcgcttcca
      661 gagaggttcc cggccgagtt tacatctatg actgtcatgg cctcggttct gtcgatatag
      721 atatttccgc cgccggggag agcaaccgtt ctggtataga gaatcttgag cttctcgtag
      781 atgtcctccg tggcgaaagc gtctccttcg acgtatctga ctaccggttt aaatccggcc
      841 ttgaacttgc ttattccggc cacaacctcg tgccatagtt cttctgaatc gacaacgatt
      901 tcttttgtac tttcggtgag tctttcacgg agaatgtact ccacaaagga aggttcttcg
      961 tagagaattt taggttttcg cccgcgcttg aaggtttcat ttatcttctt ccagttttct
     1021 ctgagccctt ccagctcgtc ttttatggtc tcctcaccga gtccgtagga ggccgtcctg
     1081 aagatcacac cctctttgct ctcgagaagc gattttgcca ccgttgttaa tctatgcctc
     1141 tcctggggat cggcgatctt cttcgaaata cctatgtgtt tcgaaaatgg tgt
//
LOCUS       DAOOLY010000442         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_158649_length_1192_cov_3.418646, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000442 DAOOLY010000000
VERSION     DAOOLY010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158649_length_1192_cov_3.418646"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..992)
                     /locus_tag="PLD15_12985"
     CDS             complement(75..992)
                     /locus_tag="PLD15_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869082.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPB64877.1"
                     /translation="MTRKPYKDLKSYLKERYGQAVHRIPLDAGFTCPNRVGGAVGCIY
                     CDPTGSGFAVDSRLSLLDQLRRGMDGLRKKGLGKFMAYFQANSNTFAPVERLRELYRQ
                     VLVEDVVILDISTRPDLVGEEVLALLDEFSGDVDVILELGLQSVNPNTLRKINRGHSL
                     SQFVDAALRAAKHNIETVAHVIVNLPWDTIEDVAEAARLLSVLGVKGVKIHSLYVVEG
                     TELARQCLAGETVLTSLEVFLERVVTFLEYLDEDVVVHRLVADPPIEGTLFGNWGFSK
                     LQLLNMIEKKMIIEGRYQGSKALKVQRGK"
     gene            complement(982..>1192)
                     /locus_tag="PLD15_12990"
     CDS             complement(982..>1192)
                     /locus_tag="PLD15_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64878.1"
                     /translation="EVSNDFRSVVSALPLIYGEFQDEIRQGYMKAWFSGKWLYVFLGK
                     TKSEVNSAVKTFKEFEKLLVKSVDS"
BASE COUNT          266 a          366 c          263 g          297 t
ORIGIN      
        1 ctccccctgg gtgttaccca gcatcctcgc ccatggagtc cggactttcc tcggcctcgc
       61 cgcgatcacc cgtttcactt tcctctctga actttgagag ctttcgagcc ctggtatctt
      121 ccttcaatta tcatcttttt ctctatcata ttcaacagtt gcagcttcga aaaaccccag
      181 tttccgaaaa gcgtcccctc gatcggcgga tcggcaacca gtctgtgaac gaccacatcc
      241 tcgtccagat actccagaaa ggttacaacc ctctcgagaa aaacttccag actcgtgagt
      301 acggtctcgc cggccagaca ctgcctggcc agctcagtgc cttcgacgac gtaaagcgaa
      361 tggattttga cccccttcac gccgagtacg gaaagcagcc tggccgcttc cgcaacatcc
      421 tcgatcgtgt cccacggaag gttgactatg acgtgcgcga ccgtctcgat attgtgcttc
      481 gccgccctca gggccgcatc cacgaactgt gagagagaat gacctctgtt tatcttccta
      541 agcgtgttgg ggttcacact ctgaagaccc aattcgagta tcacgtctac atctccggaa
      601 aactcgtcta gaagggcgag cacttcctcg ccgaccagat ctggtctggt ggaaatgtcg
      661 agaatgacca catcctctac caaaacctgt ctgtagagct ccctgagtct ttctaccggg
      721 gcaaaagtgt tcgaattggc ctgaaagtag gccatgaact tccccagacc cttttttctg
      781 agcccgtcca ttcctctccg gagctgatcg agaagcgaca acctcgaatc aacggcgaat
      841 ccactcccgg taggatcgca atagatgcat ccgacagctc cgcccactct gttgggacag
      901 gtaaatcccg cgtccagagg gattctgtga acagcctggc cgtatctctc tttgaggtag
      961 cttttcagat ctttgtaagg cttacgagtc aactgatttc accagcaact tctcgaattc
     1021 cttgaaggtt ttcactgccg aattcacttc gctcttggtc tttcccagga atacatatag
     1081 ccacttcccc gagaaccagg ccttcatgta tccctgccgg atctcgtcct gaaactctcc
     1141 gtatatcagt ggcaaggcgc taacaacgga tctgaaatcg ttggagactt cc
//
LOCUS       DAOOLY010000443         1180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_161247_length_1180_cov_3.818667, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000443 DAOOLY010000000
VERSION     DAOOLY010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161247_length_1180_cov_3.818667"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLD15_12995"
     CDS             complement(<1..570)
                     /locus_tag="PLD15_12995"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPB64879.1"
                     /translation="MPYVNTKDILDKANKGYYAVPALNINNLEFLQAIIEAGVEENSP
                     IIIETSEGAIKYAGDGDIQRGAGLFVSMVRAFADNIDIPVSLHVDHGKSFKILMAAIK
                     AGYSSIMIDASEHPFEKNIEETKKMVEIAHSVGVSVEAELGRLVGIEDNVMVESHEAA
                     LVDPDEAKQFVEETGVDFLAPAIGTSHGAF"
     gene            complement(640..>1180)
                     /locus_tag="PLD15_13000"
     CDS             complement(640..>1180)
                     /locus_tag="PLD15_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPB64880.1"
                     /translation="YPASNRTLIDKVRTSGCIVSEFLPGTPPLKQNFPRRNRIIAGLS
                     KAVFVVQAGLRSGSLITARLALENGRDVFALPGDIYRENSRGTNWLLKNGAKLITEYE
                     DILEEFPWLAREERRESENRKIDSPVYRCLGNGPMTFGEILLASGKSSNELMVELTQL
                     QLEGHVYEENGRWSRSCNL"
BASE COUNT          253 a          326 c          261 g          340 t
ORIGIN      
        1 gaaagctcca tggctagtgc ctatcgcggg agcgaggaaa tccactccgg tctcttcaac
       61 gaactgcttc gcctcgtccg ggtcaaccag tgcagcttcg tgcgattcga ccatcacgtt
      121 gtcttctatt cccacgagtc ttccaagttc ggcttccacg gataccccta cactgtgggc
      181 tatctcgacc atctttttcg tttcttctat atttttctcg aacggatgtt cggaagcgtc
      241 tatcattatc gatgagtagc ctgccttgat agccgccata agtattttga aactcttgcc
      301 gtgatcgaca tgcagagata ccgggatatc tatattgtct gcgaaggctc tcaccattga
      361 aacgaaaagg ccggctccac gctggatatc gccatcacct gcgtacttta ttgctccttc
      421 agatgtctct attattatcg gggagttctc ttctaccccg gcctctatga tggcctgaag
      481 aaattcaagg ttgttgatat tgagtgccgg cacggcatag taacctttgt tcgccttatc
      541 aagaatatcc tttgtgttaa cgtacggcat ttacgctccc tccaattctt ttgtttaagt
      601 gctgttcaaa acgattatac caccacatta accgaccatt cagaggttac agcttctgct
      661 ccagcgtcca ttctcttcgt aaacatgccc ttcaagctga agctgagtca gctccaccat
      721 cagttcgttg cttgacttcc cgctagctag aaggatctct ccgaaagtca tcggaccgtt
      781 tcccaaacac ctgtaaacgg gcgagtcgat ctttcgattt tcactttccc ttcgttcttc
      841 tctagcgagc cagggaaact cctcgagtat atcttcgtat tcggtgatca acttcgcgcc
      901 gttcttgaga agccagttgg tgcccctcga attctctcta tagatatctc ccggcagtgc
      961 gaagacatct ctcccgtttt ccagggccag gcgggcagta atgagtgagc cactcctcag
     1021 tccggcctga actacgaaaa cggcctttga gagaccggcg atgatcctgt tccgtctggg
     1081 gaaattctgc tttagcggag gcgttcccgg caggaactcc gatacgatac agccactggt
     1141 gcgtacctta tctatcaggg ttctattgga tgctggatag
//
LOCUS       DAOOLY010000444         1176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_162118_length_1176_cov_5.603033, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000444 DAOOLY010000000
VERSION     DAOOLY010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162118_length_1176_cov_5.603033"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLD15_13005"
     CDS             complement(<1..652)
                     /locus_tag="PLD15_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPB64881.1"
                     /translation="MTENEILSVRGISKSFDDLSVLKGVSFDVKQGDVIAILGPSGAG
                     KTTLLRCINHLITPDGGDILFKGSTVKGDRKSIRSFRARVGFVFQRFNLVSHLRVIDN
                     VALPLVKVLGKKWKEAREIALKQLSQVGLDDKAASFPSQLSGGQQQRVGIARALVMQP
                     DIVLLDEPTSALDPSLVGEVMRVIKRLSSEGRTMVIVTHEVDFARNIATRVLFMKDG"
     gene            complement(649..>1176)
                     /locus_tag="PLD15_13010"
     CDS             complement(649..>1176)
                     /locus_tag="PLD15_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPB64882.1"
                     /translation="LARTSHSGFFKDAGTLYVWFFRNMPLLVIILLVYYGFGSAINID
                     RFWAGVVSLSLFEGAYVGEIFRAGIEAVPAGEIEAGEAMGLYRRQIMFSIIFPQAFRI
                     SIPPLIGQLVSLVKDSSLVSVIALGDLTMRARQVGTQTLAVFESYIALALCYLLITST
                     LSFVGRALERKMVIP"
BASE COUNT          284 a          323 c          297 g          272 t
ORIGIN      
        1 agccatcttt catgaacaaa accctggtgg ctatattcct ggcaaaatcg acttcgtggg
       61 tgacgatcac catggttctc ccttccgatg agagtcgttt tatgactctc attacttctc
      121 caactagcga agggtcgagc gccgaagtcg gttcgtccag caacactata tcgggctgca
      181 tcaccagggc tctcgcgatg ccgacccgct gctgctgacc gcccgacagc tgggaaggga
      241 agctggcagc cttgtcatcc agaccgacct gagagagctg cttcagggcg atttctctgg
      301 cctccttcca ttttttgccc agaactttta ctagagggag cgcgacgttg tctatcactc
      361 tgaggtggct gacgagattg aatctctgga agacgaatcc cactctcgcc ctgaaggatc
      421 tgatagattt cctgtcgccc ttcacggtcg agcccttgaa gagtatatcg ccgccgtcgg
      481 gggtgatgag atggtttata cacctcagaa gggtggtctt tccagccccg gagggaccga
      541 gaatggcgat aacatccccc tgcttcacat cgaaagagac ccctttgaga acggaaaggt
      601 cgtcgaagga tttggaaatt cctcttacgc tgagaatttc gttttccgtc atggtatcac
      661 catcttcctt tcgagagctc tgccaacgaa cgacagagtc gatgttatta gcaggtagca
      721 aagggccaga gcgatgtaag actcgaatac ggccagagtc tgtgtgccga cctgtctggc
      781 tctcattgtg agatctccga gcgctatcac cgaaaccagc gagctgtctt tcacgaggct
      841 gaccagctgc cctatcaggg gaggaataga tatcctgaag gcctgcggga agattatcga
      901 gaacattatc tgtctcctgt aaagccccat agcctcgcca gcctcgattt ccccggccgg
      961 taccgcttcg ataccggccc tgaatatctc tcccacataa gcgccttcaa atagggagag
     1021 cgagaccact ccagcccaga agcgatcgat atttatcgcc gatccaaagc cgtagtaaac
     1081 gagtagaatg ataacaagaa gcggcatatt tctgaaaaac cacacgtaca gagtgccggc
     1141 atctttgaaa aaacccgagt gtgaagttct cgccag
//
LOCUS       DAOOLY010000445         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_163331_length_1171_cov_2.048387, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000445 DAOOLY010000000
VERSION     DAOOLY010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163331_length_1171_cov_2.048387"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="rfbF"
                     /locus_tag="PLD15_13015"
     CDS             <1..669
                     /gene="rfbF"
                     /locus_tag="PLD15_13015"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HPB64883.1"
                     /translation="HIMKLYSFYGFNDFVICLGYKGYVIKEYFANYFLHRSNITFDLA
                     ENRMVVHDSYAEPWRVTLEDTGLETFTGGRVKRIAKYVQNETFMLTYGDGVGDVNIRA
                     LLEFHKRHGKMATVTAVQPFGRFGALRINSENIVESFSEKPKGDGAWINGGFFVMEPG
                     VLDYIEGDKTILERTPLERPASEGQLGAYKHSGFWRPMDTLRDKIELESLWNSGKAPW
                     RIWE"
     gene            660..>1171
                     /locus_tag="PLD15_13020"
     CDS             660..>1171
                     /locus_tag="PLD15_13020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPB64884.1"
                     /translation="MGIDQLFGGIYNGKRVLITGHTGFKGSWLAFWLERMGAEVIGYA
                     LPPESVPNHYELIRPRLKSVFADIRESEILEKTFIETSPEIVFHLAAQPFVRRSYREP
                     LATFDTNIMGTARLLEACRKTVSVRAVVVVTSDKCYENKEWPWGYRETDPMGGLDPYS
                     ASKGCVELLSG"
BASE COUNT          314 a          267 c          309 g          281 t
ORIGIN      
        1 catattatga aactgtattc attctacggg tttaacgatt tcgtgatctg tctcgggtac
       61 aaggggtatg tcatcaaaga gtattttgcg aactacttcc tccacaggtc gaatattacc
      121 ttcgatctgg ccgagaatcg catggtggtt cacgacagct atgcggaacc ttggagagtg
      181 acactcgaag acacaggcct agaaaccttt accggcgggc gggtaaaaag aattgccaaa
      241 tatgtgcaaa atgagacctt catgcttacc tacggcgacg gcgttggtga cgtcaatata
      301 cgtgcgctcc tggaattcca taaaaggcac ggaaaaatgg cgacggtcac agcagtacaa
      361 cccttcggac gtttcggcgc actcagaatc aattctgaaa acatcgttga aagtttttcc
      421 gaaaagccaa agggagacgg tgcttggatc aatggaggat tttttgtcat ggaaccggga
      481 gtccttgatt acatagaagg agataagacg attttagaaa gaacgcctct tgagcggcca
      541 gcctctgaag ggcaactcgg agcatacaag cacagcgggt tctggcgacc gatggacacg
      601 cttcgtgaca agatagagtt agaaagcctt tggaacagcg gtaaagcgcc gtggcggata
      661 tgggaataga tcagcttttt ggtggcatct ataatggaaa acgggtactt atcaccggac
      721 acactggttt caagggctcg tggctagctt tctggctgga aagaatgggc gcagaagtca
      781 ttggttacgc gcttcctccg gaatctgtcc cgaaccacta cgaactgatt cgtccacgac
      841 taaaatctgt attcgctgat attcgtgagt ctgagattct ggaaaagaca ttcatcgaaa
      901 catcgcccga gattgttttc catctcgccg cccaaccttt tgtccgccgc tcctacagag
      961 aaccgttggc aacatttgat accaatatca tgggtacggc ccgcctgttg gaagcgtgta
     1021 gaaaaacagt ctcggtacgc gctgtggtcg ttgtgacatc agacaagtgc tatgagaaca
     1081 aagagtggcc ttgggggtat cgagaaacgg atcccatggg cggacttgac ccctatagcg
     1141 cttcgaaagg atgtgtcgaa cttctctccg g
//
LOCUS       DAOOLY010000446         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_164793_length_1164_cov_2.845807, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000446 DAOOLY010000000
VERSION     DAOOLY010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164793_length_1164_cov_2.845807"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLD15_13025"
     CDS             <1..802
                     /locus_tag="PLD15_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB64885.1"
                     /translation="EPASTLLLVGENLLDDLRLAVDITSEENRTLNGHITARFFFNIL
                     TILLLYTFGRHFLSPAMSLLSVLLFQSIMPLTTMYFGWETFHAAALFLAGLLLIIERR
                     SFATLCLVIFLGSTFRADHMVFLSLIFLGFDYGRHPGKAGKLLLSLKVLTTFAIPVAF
                     TLLSSRLLYPQAEYSVDLVQLWYNLTYPWSWIFPFLFLSIPLIFYDSIGEEDFFRKTW
                     FWIVPFVAMNYIVARPAEVRLLIPVLLYTIPVAVKGLSKFFPADVSKA"
     gene            926..>1164
                     /locus_tag="PLD15_13030"
     CDS             926..>1164
                     /locus_tag="PLD15_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB64886.1"
                     /translation="MARDLTQGSILKNLLVMSIPTMIGFSAQMVYDIVDIFWIGRISG
                     EAIAGVTIFSTLFWLVEILNSIIGQSSISLISQSYG"
BASE COUNT          242 a          310 c          275 g          337 t
ORIGIN      
        1 agaaccggcc agcacccttc tgctagtggg agaaaacctc cttgacgatc tgaggttggc
       61 cgtcgatatt accagcgagg aaaacagaac gctcaatggc cacataacgg ccaggttctt
      121 cttcaacata ctgactattc tgctgctgta cactttcggc agacatttcc tttctccagc
      181 aatgtcgctc ctttctgtat tgcttttcca gtcgataatg ccccttacga ccatgtattt
      241 cggctgggag accttccacg cggccgcgct cttcctcgcg ggactgcttc tgataatcga
      301 gagaaggtcc ttcgccacac tctgtctggt gatctttctg ggctcgactt tcagggccga
      361 tcacatggtc tttctctcgc ttatatttct cggtttcgat tacggaagac accccggaaa
      421 ggccggaaaa ctacttctct ccctcaaagt gctcacgact ttcgccatac ccgttgcttt
      481 tacgttgctt tccagccgtt tgctgtatcc gcaggccgaa tattcggtgg atctcgttca
      541 actgtggtac aacctcacct acccctggtc ctggatcttc ccattcctct tcctttcgat
      601 cccgctgatc ttctacgatt caatcgggga ggaggatttc ttcaggaaga cctggttctg
      661 gatagttccc ttcgtcgcga tgaactatat agtagccagg ccggcagagg tgcggctact
      721 cataccggtg cttctctata cgatccccgt cgccgtgaag ggtctctcga aattctttcc
      781 ggccgacgtc tcgaaagcct gaattgacgg gttcacaaag ggctgtatga gtgtataata
      841 tctattgttt cctgcccggg gcaggttttg ttgttattct cttcatctgc atcttctgaa
      901 tggggttgta atcagggggt agaaaatggc aagggatctt actcagggaa gcattctgaa
      961 gaatcttctc gtcatgtcca taccgacgat gataggtttc agtgcacaga tggtctatga
     1021 tatagtcgat atcttctgga taggcagaat atccggcgag gcaatcgccg gggtcacgat
     1081 attttcgaca ctcttctggc ttgtagagat cttgaactcc ataatcggac agagttcgat
     1141 ctccctgatc tcgcagagtt acgg
//
LOCUS       DAOOLY010000447         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_167747_length_1151_cov_4.474453, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000447 DAOOLY010000000
VERSION     DAOOLY010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167747_length_1151_cov_4.474453"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLD15_13035"
     CDS             <1..229
                     /locus_tag="PLD15_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB64887.1"
                     /translation="EVAETTGCGDSFMAAVISRLYSLSDERFKALDFETLGGILRFAN
                     AGAAIVATRYGAANAMPSGSEIGEFLQSHRR"
     gene            236..823
                     /locus_tag="PLD15_13040"
     CDS             236..823
                     /locus_tag="PLD15_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPB64888.1"
                     /translation="MKGFLAVDIGASGGKYRLLKNATGLWLVQELLREWKAKDQSLDY
                     PSLVRMAGEARPFSGKIDVESPRFKRPASMERAILQECADSSSIPCTRGEIVRTALEG
                     IAAMTELTRKELERLTGRIDGLHIVGGGVKNQLLCQLIADFTGLPVTAGPVEGTAIGN
                     IIVQMLALGELRDPGESIELLKRSFEMLSYEPRNS"
     gene            complement(842..>1151)
                     /locus_tag="PLD15_13045"
     CDS             complement(842..>1151)
                     /locus_tag="PLD15_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB64889.1"
                     /translation="LLPVLQDFFKTTVPLDTVIALLESMAKIAGLSQLTLYSIPSIMA
                     EIAGCDIDLFKGHSYNDIEVERIARLIKFAVDNFVPEKLEGSQYRAFREGFLELFPAQ
                     "
BASE COUNT          274 a          303 c          338 g          236 t
ORIGIN      
        1 cgaagttgcc gagacgaccg gctgtgggga ttcattcatg gccgccgtca tatctagact
       61 ctactcgctc tcagatgaac ggttcaaagc cctcgatttt gaaacgctcg gagggatcct
      121 gagattcgcc aacgccggcg cggcgatcgt agcgacaagg tacggagcgg caaacgccat
      181 gccgtcaggg agcgagatcg gggagtttct ccagtcccat cgaaggtgat cccgcttgaa
      241 aggttttctc gccgtcgata tcggtgcctc tggcggcaaa tacaggcttt tgaaaaacgc
      301 cactggcctt tggctggtgc aggaactgct cagggagtgg aaagcgaaag accagtcgct
      361 ggactacccc tcgcttgtgc gtatggccgg ggaggcgagg ccgttctccg gaaagataga
      421 cgtcgaatcg ccccgtttca agagaccggc aagcatggaa agggcgattc tgcaggagtg
      481 tgccgatagc agttcgatac cctgcacacg gggtgaaata gtaaggacgg ctctggaagg
      541 catagccgcg atgacggaac tcaccaggaa ggagctggaa cggttgaccg gcaggatcga
      601 cgggctccac atagtgggcg gaggagtgaa gaaccaactg ctctgccagc tgatagccga
      661 tttcacgggc cttcccgtca ccgccggacc ggtagagggg acggcgatag gaaacataat
      721 cgtccagatg ctcgcgctcg gcgagttgag ggatccagga gaatcgatcg aattgctgaa
      781 aagatccttc gaaatgctat cttacgagcc gcgaaattct tgacaggccc cccgaccgct
      841 actattgcgc gggaaagagc tccaagaacc cctctctgaa agctctgtac tgacttccct
      901 ccagtttttc cggcacaaaa ttgtcaacag cgaatttgat gagccttgcg attctctcga
      961 cctctatgtc gttatagctg tggcctttga agagatcgat gtcgcagcca gctatttcgg
     1021 ccattatcga gggaatcgaa taaagcgtca gttgcgacaa tccggcgatc ttcgccatac
     1081 tttccagaag ggcgatcacc gtatcgagcg gtaccgttgt tttgaagaaa tcctgcagaa
     1141 cgggcaacag c
//
LOCUS       DAOOLY010000448         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_172127_length_1133_cov_2.600186, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000448 DAOOLY010000000
VERSION     DAOOLY010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172127_length_1133_cov_2.600186"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1059
                     /locus_tag="PLD15_13050"
     CDS             142..1059
                     /locus_tag="PLD15_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869082.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPB64890.1"
                     /translation="MIHKPYRDLKSYLKERYGQAVQRIPLDAGFTCPNRVGGAGGCIY
                     CDSTGSGFTVDSRLSLLDQLQKGMEGLRKKGLGKFMAYFQANSNTFAPVERLRELYKQ
                     VLIEDVVILDISTRPDLVGEEVLELLDELSRDIDVILELGLQSVNPNTLKKINRGHTL
                     SQFVDAAIRATKHNIETVAHVIVNLPWDTIEDVAEAARLLSVLGVKGVKIHSLYVVEN
                     TELARQYLAGETVLTSLDVFLERVVTFLEYLDEDVVVHRLVADPPIEGTLFGNWGFSK
                     LQLLNMIEKKMIIEGRYQGSKALKVKRGK"
BASE COUNT          314 a          240 c          322 g          257 t
ORIGIN      
        1 tccagggcga ggtacagaag ggatacatga gggcctggtt ctcagggaag tggctttacg
       61 tattccttgg aaagaccaag agcgaggtga actcgatggt taaagccttc aaggaattcg
      121 agaagttgct ggtgaaatca attgattcat aagccttaca gagacctgaa aagctacctc
      181 aaggagagat acggccaggc cgttcaaagg atccctctgg atgcgggatt tacctgtccc
      241 aacagagtcg gtggagctgg tggatgcatc tattgcgatt ctaccgggag tggattcacc
      301 gttgattcaa ggctctcact tctcgatcag ctccagaaag gaatggaggg gctcagaaaa
      361 aaagggcttg ggaagttcat ggcctacttt caagccaact cgaacacctt cgccccggta
      421 gaaaggctca gagaactcta taaacaggta ttgatagaag atgtggtcat tctcgacatt
      481 tctaccagac ccgatctggt cggcgaggaa gtgctcgaac ttcttgatga actctccaga
      541 gatattgatg tgatacttga gctgggtctt cagagtgtaa accccaacac gcttaagaag
      601 ataaacaggg gtcacactct ctcacagttc gtagatgcgg ctataagggc gacaaagcac
      661 aacatcgaga cagttgcaca cgtcatagtc aacctacctt gggatacaat cgaagatgtc
      721 gcggaggcgg ccagactgct ttctgtactc ggtgtgaaag gggtaaaaat ccattcactt
      781 tacgttgtcg aaaacaccga gctggccagg cagtatctgg ctggtgagac cgtactcacg
      841 agcctggacg tttttctcga gagggtggtt acttttttgg agtatctgga cgaggatgtg
      901 gtagttcaca gactggtcgc cgatccgccg atcgagggga cgcttttcgg aaactggggt
      961 ttttcaaaat tacagctgtt gaatatgata gagaaaaaga tgataatcga aggtagatac
     1021 cagggttcga aagccctcaa agttaagaga ggaaagtgaa acgggtgatc gcggcaatgc
     1081 cgaggaaagt ccggactcca tgggcgagga tgctgggtaa cacccagggg gag
//
LOCUS       DAOOLY010000449         1102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_179884_length_1102_cov_3.460363, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000449 DAOOLY010000000
VERSION     DAOOLY010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179884_length_1102_cov_3.460363"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLD15_13055"
     CDS             <1..198
                     /locus_tag="PLD15_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPB64891.1"
                     /translation="IVQSLMSLGMGKGPALALFMAGNSLSLPSMIVITKLLGKKKAFT
                     YFGLVVAFATIWGYIYGNLLQ"
     gene            210..446
                     /locus_tag="PLD15_13060"
     CDS             210..446
                     /locus_tag="PLD15_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPB64892.1"
                     /translation="MKVEVFGSGCPRCKQTEKVMKMAVEELGLNAVVEKVTDMMAMME
                     KGIISTPAVALDGEIVLSGKIPSLEEAKKLLKRL"
     gene            776..>1102
                     /locus_tag="PLD15_13065"
     CDS             776..>1102
                     /locus_tag="PLD15_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB64893.1"
                     /translation="MVGIIVDSGCDLPEAIKEKKNVRVIPLKVVLEGKEYRDNIDITT
                     EQLLEFMENKFPKTSLPGYNDISQAFESLYREGMKEIIFVGISSGLSGTLGLVKRYSE
                     EFIKKNP"
BASE COUNT          307 a          243 c          290 g          262 t
ORIGIN      
        1 atagtccagt ccctaatgtc cctggggatg ggaaaagggc cggctctggc cctcttcatg
       61 gccggcaact ctctcagcct gcccagtatg atagttataa caaagctgct gggaaagaag
      121 aaggccttca catatttcgg cctcgtggta gcttttgcga ccatatgggg ttatatatac
      181 ggcaacttac ttcaatagga ggttagataa tgaaggtaga ggtttttggt tccggctgtc
      241 ccagatgcaa acaaacggaa aaggtaatga agatggcggt cgaagaactc ggcttgaacg
      301 cggtcgttga aaaagtgacg gatatgatgg ccatgatgga aaagggaata atatcgaccc
      361 cggccgtggc gctcgacgga gaaatagttc tctcgggcaa aatcccttcg ctggaggaag
      421 cgaagaagct cctgaagaga ctttgaaata ccttgcttta cccctcgctt tccactgacc
      481 gtggattaag accgggagtt acatatcgtt cccgcttcga agttcgacaa aattggtacg
      541 tcgatcgaca catccggtta cacccgaatg tttttcgcag ttctgccgga acgatttcga
      601 tggctctgga tagaatacat acatgatcac accggaccag agcttctatt attgccccgc
      661 cagggcgata ttcaagctat aattaaaaga tgtacccggt gaacgctgcc ggagtttccg
      721 gcgtctcaaa tcttgtgtga cgggtaatta ctatacttta cagggggtcg attgtatggt
      781 tggcataatc gtcgattcgg gctgcgatct tccagaagcg atcaaagaga agaaaaatgt
      841 gagggtaatt ccgctcaagg tagtgctcga gggaaaggaa tacagagaca acatagatat
      901 cacgaccgaa cagctgctgg agttcatgga gaacaaattt cccaagactt cgcttcccgg
      961 ttacaacgat atcagtcagg cctttgagag cctttacaga gaaggaatga aagagataat
     1021 cttcgtcggg atctccagcg ggctcagcgg aactctgggg ctcgtgaaaa gatactccga
     1081 agagtttata aagaagaatc cc
//
LOCUS       DAOOLY010000450         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_185628_length_1081_cov_1.673489, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000450 DAOOLY010000000
VERSION     DAOOLY010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185628_length_1081_cov_1.673489"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1081
                     /locus_tag="PLD15_13070"
     CDS             <1..>1081
                     /locus_tag="PLD15_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Two component regulator three Y domain protein"
                     /protein_id="HPB64894.1"
                     /translation="RTLVANFEEEVETGAFKVANSWGIGGWENVPDGFLYITYEAMKK
                     NRVVCFITDPRNGYEPRAIAVFEISHPVRDDCRVYVGVGDPASPLREKSFDVYDPDYR
                     GGPLPFPENKMVLDITELLPFNDETVYLKVSDSSKTSSTGVIESFSVEVYNNYPSGIP
                     TDVFTSTQTPKNTVNDSSVNVQIPSVTYASSPFYDLQYDGGAGISPELLARMKEQMGI
                     YEEGVNYNEIIDGFGTGLRPPTEEEWEEISINWREANGFITQDALPAMIDYSSSIYFP
                     PIGNQGSEGSCVAFSIGYYISTFYEARDRGWNLSGAQWVGDSKGSPTNSYQNRIFSPD
                     FIYHQINNGVDEGAHYVNAMKVISNI"
BASE COUNT          300 a          259 c          284 g          238 t
ORIGIN      
        1 cagaacactg gtggctaact ttgaggaaga ggttgaaact ggagccttca aagtcgccaa
       61 ctcatgggga atcgggggat gggaaaatgt accagacggc ttcctgtaca tcacatacga
      121 agccatgaaa aagaaccgcg ttgtatgctt cataacagat ccgagaaacg gttacgagcc
      181 cagggcgatt gccgtgttcg aaatatccca tcctgtcagg gacgactgcc gggtatatgt
      241 gggtgtcggc gatcccgctt cgcctttgag agaaaagagc ttcgatgttt atgatcccga
      301 ttaccgtggc ggaccgttgc ccttcccgga gaacaaaatg gtgctcgata taacggaatt
      361 gctcccgttc aacgatgaaa ccgtgtacct gaaagtctcc gactcttcga aaacctcgag
      421 tacgggcgtt atagagtcat tctcggtcga ggtgtacaac aactacccgt ctggaattcc
      481 cactgacgtt ttcacttcca cgcaaacacc gaagaacact gtgaacgact cgagcgtgaa
      541 tgtgcagatc cccagcgtga cttatgcttc gagtccgttc tacgatctcc agtacgacgg
      601 tggagcgggg atttctccgg agctactggc aagaatgaaa gaacagatgg gtatatacga
      661 agagggagtg aattacaacg agataataga tggcttcggc acgggcctaa gaccgccaac
      721 ggaagaagaa tgggaagaga taagtatcaa ctggcgcgaa gcgaatggtt tcatcactca
      781 agacgcatta ccggccatga tcgactactc cagttcgatc tatttcccgc cgataggcaa
      841 ccagggcagc gaaggttcct gcgtcgcttt ctcgatcggt tattatatat ctacgtttta
      901 cgaggctcga gacaggggct ggaacttgag cggagcacaa tgggtgggcg acagcaaagg
      961 ttctccaacg aattcatacc agaacaggat cttcagtcca gatttcattt atcaccagat
     1021 aaacaacggc gttgacgaag gcgctcacta tgtcaacgcg atgaaagtga tctcaaacat
     1081 a
//
LOCUS       DAOOLY010000451         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_187963_length_1072_cov_3.714848, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000451 DAOOLY010000000
VERSION     DAOOLY010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187963_length_1072_cov_3.714848"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLD15_13075"
     CDS             <1..990
                     /locus_tag="PLD15_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB64895.1"
                     /translation="SHEALDILFDLISSPRFEESAIELERGVILEEIASAEDDPYDRI
                     YDMTIQKVWDRDFGRPILGYDSTVKVLTREDISNFYEHKYIADRAIFAVTGNYGDSLI
                     DRAQEKLLSLQKNNKETSPTKSPVISKEPLWIVEKRKDLQQVHLLLTKGAPGRRNRDD
                     FEAFKVFNILFGSGMSSILFHNIREELGMVYNINSEFVSYTESGAFMINASTNPKNLD
                     DLIGSLENELHRIVEKGVSQAQFNYGTERLRGKLLMSTEGTLSTLSRFLDDVVICGRP
                     DSLETLIEKIDDLTIEELNNIARKYLSGSWNVSLLLPKNMPDSSFVARHGFSI"
BASE COUNT          308 a          260 c          266 g          238 t
ORIGIN      
        1 tcgcacgaag cgctggatat tcttttcgat ctgatatctt ctccacgttt cgaagaaagt
       61 gcgatagagc tggaacgcgg agtcatactc gaagagatag cttcggccga agacgaccct
      121 tacgacagga tttacgacat gacgatccag aaagtctggg acagggactt cggacggcct
      181 atactcggat acgacagcac cgtcaaggtt cttaccagag aggatatttc gaatttctac
      241 gaacacaaat acatcgccga cagggcgatc ttcgcggtta cgggcaacta cggcgattct
      301 ttgatcgaca gagcacagga gaagcttctt tctttacaga aaaataacaa agagacttcc
      361 ccgaccaaat ctccggtgat ttcgaaggaa ccgctctgga tagtggagaa aaggaaggat
      421 ctgcagcagg tccacctgct tctgaccaaa ggggcaccgg gaagaaggaa cagggacgat
      481 ttcgaagctt tcaaggtgtt caacatactt ttcggaagcg gcatgagctc gatcctgttt
      541 cacaatatac gcgaggagct cggcatggtt tacaacataa attctgagtt cgtctcttac
      601 accgaatccg gggccttcat gatcaacgcc tccaccaatc ccaaaaacct cgacgatctg
      661 attggatcgc tcgaaaatga actccacaga atagtagaga agggtgtcag tcaggctcag
      721 ttcaattacg gaaccgaaag gctcaggggc aagctcctga tgtccaccga aggaaccctc
      781 tcaacgctga gccgctttct ggacgacgtt gttatatgcg gtcggcccga ttcgctcgag
      841 actctcattg aaaagataga tgacctcaca atcgaagagc tgaacaatat cgccagaaaa
      901 tacctgagcg gtagctggaa cgtctccctt ctgttgccga aaaacatgcc agactcctct
      961 tttgtagcca gacacggctt ctcaatctga gaatgaattc actgacaggg ggtaatcagg
     1021 aaaccaatct gtaacttgac agtgaaggga ggtactggta gtataggaat tg
//
LOCUS       DAOOLY010000452         1071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_188221_length_1071_cov_4.558071, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000452 DAOOLY010000000
VERSION     DAOOLY010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188221_length_1071_cov_4.558071"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLD15_13080"
     CDS             complement(<1..338)
                     /locus_tag="PLD15_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPB64896.1"
                     /translation="MLFKDNYLWKLGFLAVGLQAITVLFVAQMGWIRIAPPTLNFFGN
                     IVGAYVFGLGMVLAGGCASGVTYRAGEGMTTAMFGAVFYGLGALAMRGGVFSGIRTWA
                     AGFNITVAGNS"
     gene            complement(448..687)
                     /locus_tag="PLD15_13085"
     CDS             complement(448..687)
                     /locus_tag="PLD15_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPB64897.1"
                     /translation="MAKYAVTKSIDVRGEVCPVPDVETKRALKKMKAGEILEVWIDYA
                     MSKERIPEAVKNLGHEILEIEETGTSEWKIYIKVK"
     gene            977..>1071
                     /locus_tag="PLD15_13090"
     CDS             977..>1071
                     /locus_tag="PLD15_13090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPB64898.1"
                     /translation="MEFVKSGFVHVYTGNGKGKTTAAFGMALRAAA"
BASE COUNT          297 a          280 c          217 g          277 t
ORIGIN      
        1 gagtttccag caacggtaat gttgaacccg gccgcccatg tccttatacc tgagaaaact
       61 ccacccctca tcgcgagtgc gcctagaccg tagaaaacgg ctccgaacat cgcggtggtc
      121 attccctctc cggccctgta cgtgactcca gacgcgcatc caccggcaag aaccatacca
      181 agaccgaaaa cataagctcc cactatatta ccaaaaaagt tcaaagtcgg gggagctatc
      241 cttatccagc ccatctgggc aacgaagaga actgtaatag cctgaagtcc taccgcgaga
      301 aatcccagtt tccacaggta attgtcctta aacagcaaaa tatctctgaa agccgagtta
      361 aaacaaactc ttccacgttg aagaatgata ccgaatacta cacctactaa gagacccgtc
      421 caagccatag tcattcactc ccgtctatca cttgactttt atgtagatct tccactcgct
      481 ggtgcctgtt tcttcgattt cgagaatctc atgaccgaga ttcttaacgg cttcgggtat
      541 tctctctttc gacatcgcgt aatcgatcca gacttctaga atctctccgg ctttcatctt
      601 cttgagagct cttttcgttt caacatccgg tacgggacag acttctcctc ttacatcgat
      661 tgactttgtt acagcatact tcgccatatt catacctcca gtttggtgtg aaataattca
      721 cattatgata ttaaaaataa aatctcatgg gcaacctggc aattgaaaat tgaacggaat
      781 tcaaggtcgg tacaacagaa tgataacctc ccacagttac ccaatatttg accgtttatg
      841 acagcttggt aaattaaggt gcagtttcgg aggtaatgag tttgaacgaa atctttctcc
      901 cctggtcggg tcattttctt ggcaggatgc tagaatcgca ctgagcattt ccgatataaa
      961 atctatggag gtcggcatgg aattcgtaaa atcgggattc gtacacgtct atacaggaaa
     1021 cgggaagggc aagacaacag cggccttcgg catggccctc agggccgccg c
//
LOCUS       DAOOLY010000453         1050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_194699_length_1050_cov_1.678392, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000453 DAOOLY010000000
VERSION     DAOOLY010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194699_length_1050_cov_1.678392"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..507)
                     /gene="rpiB"
                     /locus_tag="PLD15_13095"
     CDS             complement(49..507)
                     /gene="rpiB"
                     /locus_tag="PLD15_13095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPB64899.1"
                     /translation="MKLSIASDHAAFELKFFIAEYLKKGHEVIDFGPESPERSVDYPD
                     FARRVCKSVLSGETEFGILLCGTGIGMSMAANKYRGIRAALCLFPEMAALARKHNHAN
                     VLVLGGRLMGSELAAWTVDTFLSTVQEGGRHTRRVEKIESLPVEDLERSK"
     gene            complement(554..>1050)
                     /gene="lexA"
                     /locus_tag="PLD15_13100"
     CDS             complement(554..>1050)
                     /gene="lexA"
                     /locus_tag="PLD15_13100"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPB64900.1"
                     /translation="HLESLEKKGYIERTGVSRGIRVLKSPDGTSIEPGSDVVMLPVIG
                     NIAAGEAVQAIQAEEENIPVPLWMIRRGFEYYILKVTGNSMIDSHIISGDFVIIRKQE
                     WANNGDIVVALIDEEYATLKRYENEGQKIRLIPSNPDMLPMVIETSRVKIQGKLSGIL
                     RWYK"
BASE COUNT          242 a          280 c          228 g          300 t
ORIGIN      
        1 gttccttcat gggaagatga aagaggtggc gtctgtcgta atagatcttc atttagatcg
       61 ctccagatct tcgactggga gcgattcgat cttttcgacc cttctggtat gcctgccgcc
      121 ctcctggacg gttgagagaa acgtgtcaac cgtccatgct gcgagttcag aacccataag
      181 ccggcctcca agcaccagta cattggcgtg gttgtgtttt ctcgccagcg cggccatctc
      241 cggaaagaga cagagagccg ccctgattcc cctgtatttg ttcgcggcca tcgacattcc
      301 aattcccgtt ccgcaaagaa gaatcccaaa ttcagtttca ccgctcagca cagacttaca
      361 gacacgacgc gcgaagtctg gataatcgac ggacctttct ggcgattcgg ggccaaaatc
      421 aatgacttcg tggcctttct ttagatactc agcaataaag aatttcaact caaaagcagc
      481 gtgatcagat gctatggata atttcattat atcctccgcg catgttggga cagatttggc
      541 ttttattacg agatcacttg taccagcgga gaattccgga tagttttccc tgaatcttca
      601 ccctgctggt ttcgataacc atcggcaaca tatctggatt tgatggtatc agccgtatct
      661 tctgcccttc attctcgtat ctcttcaaag tggcgtactc ttcgtctatc agagccacta
      721 cgatgtctcc gttgttagcc cattcctgtt ttctgattat cacgaaatca ccgctgataa
      781 tatgtgaatc gatcatgctg ttaccggtaa ctttcagaat atagtactcg aagccgcgcc
      841 ttatcatcca cagtggaacg ggtatattct cctcttctgc ctgaatggct tgaacagctt
      901 ctcccgccgc tatattaccg atcacgggaa gcattacgac atcgctaccc ggttctatcg
      961 atgtgccgtc agggcttttg aggactctta ttcctctcga aacaccggta cgttcgatgt
     1021 aacccttctt ctccagagat tcaaggtgtt
//
LOCUS       DAOOLY010000454         1022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_203368_length_1022_cov_3.062048, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000454 DAOOLY010000000
VERSION     DAOOLY010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203368_length_1022_cov_3.062048"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLD15_13105"
     CDS             complement(<1..200)
                     /locus_tag="PLD15_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB64901.1"
                     /translation="MKRALGMGEIMIQMNPTEKGALRFQTLFERHVAGSEANTIIQMQ
                     RLGIECSFLTSVGADEFGKVILS"
     gene            complement(212..>1022)
                     /locus_tag="PLD15_13110"
     CDS             complement(212..>1022)
                     /locus_tag="PLD15_13110"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPB64902.1"
                     /translation="KCYLNLIDFLSQKVHGLFVCGTYGSGPLMSVEERKHVTELAVEG
                     AKGRVPVIAHIGGTSPDDIFELAKHAKEAGAMAVATVVPFYFSYSQADIVRFFTKLVE
                     ISSLPVFIYNNPKTTGVTIDIDTLKKLQAVGLYGIKDSSFDLLYFYAVKHNMDIDKFC
                     YITGTEAFIVPAVPLGAKAAICGLANALPEPVVELYNETSAANYARALELQVRVNELR
                     DVQHYAQSIPAIHAMLKMRGIHSGYPRMPYSPVDDSIYIKMEKTLIDKKAL"
BASE COUNT          243 a          250 c          240 g          289 t
ORIGIN      
        1 gaaaggatca ccttaccaaa ttcatcggcc ccaaccgatg tcaggaagga acactctatt
       61 cccaggcgct gcatctggat gattgtgttc gcttcagaac cggccacgtg cctctcgaaa
      121 agcgtttgga atctgagagc tcccttttcc gtcggattca tttgaatcat tatttcgccc
      181 ataccgagag ctcttttcaa agcatcaccc cctagagcgc cttcttatcg atgagtgtct
      241 tttccatttt tatgtagatc gaatcatcaa ccggactgta cggcattctc gggtagccgg
      301 agtgaatgcc tctcattttg agcatggcat ggattgcagg tatgctttga gcgtagtgct
      361 gcacgtctct caattcgttt accctcacct gaagttccag cgctctggcg tagtttgctg
      421 ccgatgtttc gttgtagagt tcgactaccg gttcgggtag tgcgttcgcg agaccacaga
      481 tagccgcttt ggctccgagc ggaactgccg gcactatgaa ggcttctgtt cctgttatgt
      541 agcagaactt gtctatatcc atgttgtgtt tcacagcata gaagtataac agatcgaaag
      601 acgagtcttt gataccgtat aatccaactg cctgaagctt tttgagagtg tcaatatcga
      661 ttgttacacc tgtagttttg ggattgttgt aaatgaagac gggtaagctc gagatttcga
      721 ccagtttggt gaagaatctt acaatatcgg cctgcgaata agaaaagtaa aatggaacaa
      781 cggtcgccac ggccatcgct cctgcctctt tcgcatgctt tgccagttcg aagatatcat
      841 cagggctggt cccaccgata tgtgctatta cggggaccct gcctttagca ccttcgaccg
      901 caagttcggt tacgtgtttt ctttcctcga ccgacatgag cggccctgaa ccgtaagtgc
      961 cacaaacaaa aagaccgtgt accttctgag agagaaaatc tatcagattt agatagcact
     1021 tg
//
LOCUS       DAOOLY010000455         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_203986_length_1020_cov_3.223834, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000455 DAOOLY010000000
VERSION     DAOOLY010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203986_length_1020_cov_3.223834"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="PLD15_13115"
     CDS             <1..>1020
                     /locus_tag="PLD15_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPB64903.1"
                     /translation="VKMDLKSFSRNYMYLLSKLAEEGRDLSERAIEYFWTNMLLRKLS
                     FRDNFLDIFREFNLFNKSGRDRAELIEKALKEIISRYPGYGFIEYGGKYLWIFTEGPA
                     PLRKACTYADGFIDGLAMTDIASVCGDIYNTLFSLSIDLGLVDPDAAVAMPVVEAMAK
                     LLDLKPGLSVYDPALNYGRVIYSIFKKEGLENTDFAGLETDPFRHRVGKTALAILGVN
                     TVNLKRGEPLFRPANASLTDNALYDRVVIEPPSAERIIHLRLNTGHFSMGELEIAREP
                     GSQWEYIGHGLEATKPSGKLVSLIDSCKIPEALRSIVDGDLLEAVIALPGSLVCSGGE
                     RKSIL"
BASE COUNT          241 a          277 c          304 g          198 t
ORIGIN      
        1 aggtaaagat ggatctgaag agtttttccc gcaactacat gtatctactc tcaaaactcg
       61 cagaagaggg ccgcgacctt tccgaaaggg cgatagagta cttctggacg aacatgctgc
      121 tcagaaagct ctcgttccgc gataatttcc tcgatatttt cagggagttc aatctcttca
      181 acaagagcgg ccgcgatagg gcggagctga tcgaaaaggc tcttaaagaa ataatcagca
      241 gatatccggg ctacgggttc atcgaatacg ggggcaaata cctctggatc ttcacggaag
      301 gcccagcgcc gctgcggaag gcctgcactt acgcggacgg tttcatagac ggcctggcga
      361 tgaccgacat cgccagtgtc tgcggggaca tttacaacac cttgttctcg ctctcgatcg
      421 atctggggct ggtcgatccc gacgcggccg tggccatgcc tgtcgtcgag gccatggcga
      481 agctgctcga tttgaagccg ggattgagcg tctacgatcc ggcgctcaac tatgggcggg
      541 tgatatacag catattcaag aaggagggtt tggagaacac ggatttcgcc gggctggaaa
      601 cggacccctt caggcacagg gtaggcaaga cggcgctggc catcctgggg gtaaacaccg
      661 tgaacctcaa gcggggagag ccgctcttca gacccgcgaa cgcctctctc accgataatg
      721 ccctctacga cagagtcgtc atagaaccgc cgtcggccga gagaataata catctccggc
      781 tgaatacagg acacttttcc atgggagagc tcgagatcgc gagagagccg gggagccagt
      841 gggagtatat cggccacggc ctcgaagcga caaaaccttc tggaaagctg gtcagcctga
      901 tcgacagctg caagatcccc gaagccctgc ggtcgatcgt ggacggcgac ctgctggaag
      961 cggtcatagc gctccccgga agtctcgtat gctcgggcgg cgagcggaag tccatactgg
//
LOCUS       DAOOLY010000456         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_204716_length_1018_cov_2.370717, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000456 DAOOLY010000000
VERSION     DAOOLY010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204716_length_1018_cov_2.370717"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1018)
                     /locus_tag="PLD15_13120"
     CDS             complement(<1..>1018)
                     /locus_tag="PLD15_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPB64904.1"
                     /translation="VVPLLSEGDFIGFFLAADREEVEGEFRPYKHLDEEDVRLLDDVG
                     RRTGVAISKIRLTSRLELEVRKLKQLSKRHEESISEQKDQLFKLGALHKISQAMRKSL
                     DYERIVNILLVGATHSAGLRFDRALFLKKNSRMALLTVEAKIVRGEGEFERTVYGDLS
                     RYLQDVSLERHFIAEKDNHISVSYLGNMILERVVNRRKIVHVTPDMGRFRSEELSILN
                     KIVGAQDYLVAPVSGEKDVQGVIVVDKVLTGNKISNADMEILGLLADSAGVALELTGN
                     YERLLEITDSLEKERNLSNYYRKFVSSILQSLESSIVVCSPSGEITEVNRTAELLFDA
                     RREEL"
BASE COUNT          229 a          288 c          214 g          287 t
ORIGIN      
        1 caactcctcc cgccttgcgt cgaacagaag ctcggcggtg cgattaactt cggttatctc
       61 tccggacgga gaacagacca caatcgacga ctcgagagac tgaagtatac ttgacacgaa
      121 tttacggtaa tagttggaaa ggtttctttc tttctccagg ctatcggtta tctccagtaa
      181 cctttcgtaa ttccccgtca attcgagagc caccccggcg ctgtcggcca gcaaacccag
      241 tatctccatg tcggcgttgc ttattttgtt tccagtcagg actttatcga ccactatgac
      301 accctgaaca tccttttctc cggaaaccgg cgccaccaga taatcctgtg ccccgactat
      361 tttatttagt attgagagct cttcgcttct gaaacgaccc atgtcgggtg tcacatggac
      421 aatctttcga cggttgacga ctctctcgag aatcatgttc ccgagatatg aaacggaaat
      481 gtgattatct ttttcggcta tgaagtgacg ctccagcgag acatcctgta gatacctaga
      541 aagatcgccg tacacggtcc tttcgaactc gccttcgccc ctcacgatct ttgcctccac
      601 tgttagaagc gccatgcgcg agtttttctt cagaaacaag gctctatcga acctaagacc
      661 agccgaatgt gtcgctccga ccaggagaat atttacaatt ctctcataat cgaggctttt
      721 cctcattgct tgagatatct tgtgaagcgc gcccagtttg aatagttgat ccttttgctc
      781 gcttatcgat tcctcgtgtc tcttgctgag ctgcttcagt tttctgactt ccagttccag
      841 tctcgaagtt agtcggatct tgcttatcgc gacgcccgtt ctccgaccca catcatcgag
      901 caacctcacg tcttcctcgt ccagatgttt gtaaggtctg aactcccctt ctacttcttc
      961 ccggtcggcg gccaaaaaga agcctatgaa atcgccttca gagagtagag gtaccacg
//
LOCUS       DAOOLY010000457         1017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_205033_length_1017_cov_2.288981, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000457 DAOOLY010000000
VERSION     DAOOLY010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205033_length_1017_cov_2.288981"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1017
                     /locus_tag="PLD15_13125"
     CDS             <1..>1017
                     /locus_tag="PLD15_13125"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HPB64905.1"
                     /translation="MENTVLLLSPLAPGGSEDTNFSFTIRSNTEISGFTVKAEADSIE
                     TPKSYDTANLTTPVNYKSLIEIDKTAVVNDLITKVPSGFTIVPPTLERNWDLTDPDTL
                     KYSLVLKNMSAGPGTYYLTNTATVTEDDTGEKHSDNATVTITVDEDPDLVIVNEHDMK
                     WDQERVYYWSIEKNADPNELELEAEEDGTFTYTLVATRTYEETNTSTITGMATVTNTG
                     NVALSSISVEIILEDSLGVQIDKYTDNIATLAIGAQKEIPYSFSFDPTSYNAPFKVTA
                     KAIAGSISDETEDTQNLPTANLSEIDKDAYVKDTFDPFDIDGIALEGNDYREWFNIIE
                     GSDWN"
BASE COUNT          334 a          241 c          217 g          225 t
ORIGIN      
        1 atggagaaca ccgtattact cctatcccca cttgctccag gcggcagcga agatacgaac
       61 ttctctttca caatcaggag taatacagaa atttctggat ttactgtgaa ggctgaagcc
      121 gattcaatag agactccgaa atcatatgac accgctaatc tcacgacacc ggttaattac
      181 aagagtctca tagagatcga caagactgct gtggtaaatg acctgataac taaggttcca
      241 tcgggtttca ccatagtacc tcccacgctt gaaagaaact gggacctcac agatccagat
      301 actttgaaat actcgttagt gttgaaaaat atgagtgcgg gaccaggtac gtactacctg
      361 acaaatacgg cgacagtgac ggaagacgat acaggtgaaa aacactcgga caatgcaacc
      421 gttaccatca cagtggatga agacccggat ctcgtcatag tcaacgaaca tgacatgaaa
      481 tgggatcaag agagagtata ttactggtcg atagagaaga atgccgatcc aaatgaactg
      541 gaactcgaag ccgaggaaga cggtactttc acttacaccc ttgtggcaac aagaacctac
      601 gaagaaacca atacctctac tattacaggc atggcaactg taactaacac gggcaatgtt
      661 gcgctttcca gcatctcagt ggaaataatc ctcgaagact cactcggtgt gcagatagat
      721 aaatataccg acaacattgc tacactggca atcggtgctc agaaagagat tccatactct
      781 tttagcttcg accccacatc ctataacgca ccgttcaaag taacagctaa ggccatagcc
      841 ggttcaatat ccgatgaaac tgaagataca cagaatctgc ctacggctaa tctgtcggag
      901 atcgataagg acgcctacgt aaaagatacc ttcgatccat tcgacattga cggcattgct
      961 ctcgaaggaa acgactatcg tgaatggttc aacataatag aaggctcaga ctggaac
//
LOCUS       DAOOLY010000458         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_208328_length_1007_cov_2.977941, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000458 DAOOLY010000000
VERSION     DAOOLY010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208328_length_1007_cov_2.977941"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PLD15_13130"
     CDS             complement(<1..970)
                     /locus_tag="PLD15_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB64906.1"
                     /translation="MRKPTIRDVARLSGVGIGTVSRVLNGGSVKPSTKEKVTKAIAKL
                     GYRPSSVARRLAGGRTRRVLFFMPEVRTEFHWRVMKAFDDAIDAMNYEMVIYPLFSGR
                     RLERLHNDTTLLRDSDGAVFCTMNMEFLLKRRIDFGKNIVLLESQSEDFDCVYLDNFL
                     GGKYAAQLLLDNGVKEFFTVTFEESNNVLATENFEKRLLGFSKTLEAAGYRFGKDHAV
                     YTSFFFDTAHERIADILLNYERPGIFALADNFALEVLQTASAVKKTPGKDFFLVGYDN
                     QSWTERAGLTTIQQPMEEMGRKAAELIINRIEEPVGYIQPVKFLPFV"
BASE COUNT          252 a          257 c          220 g          278 t
ORIGIN      
        1 ccacaaatgg tagaaacttg accggttgaa tatagccaac cggttcttcg attctattaa
       61 taatgagttc ggccgctttt cgtcccatct cctccatcgg ttgctggatc gtcgtaagtc
      121 cggccctttc tgtccagctc tggttgtcgt aaccgaccag gaagaagtcc tttccaggtg
      181 tcttcttaac ggccgaagct gtttgaagaa cttcgagcgc gaagttgtcg gctagtgcaa
      241 atatgcctgg tctttcgtaa ttgagaagaa tgtcggctat cctttcgtgg gctgtgtcga
      301 agaagaagga agtatatacg gcatgatcct ttccgaatct atagccggcg gcttcgagcg
      361 ttttcgaaaa acctagcaac ctcttttcaa agttctccgt tgccagtacg ttgttactct
      421 cctcaaaagt aacggtgaaa aactccttta caccgttatc gagcagtaat tgggcagcgt
      481 attttcctcc gagaaaattg tccagataga cacaatcgaa atcctccgat tgtgattcca
      541 gaagcactat attcttccca aaatcgatcc tgcgcttcag aaggaactcc atgttcatcg
      601 tacagaaaac tgctccatcg gaatctctga gaagtgtggt gtcgttgtgc aacctttcca
      661 gtctccgacc ggagaagagc ggatatataa ccatttcgta gttcatcgcg tcaatcgcgt
      721 cgtcgaatgc cttcatcaca cgccagtgaa actcggttct cacttcaggc atgaaaaaga
      781 gcaccctcct ggttcgcccg cctgccagtc gtcgggcaac tgagctgggt cgataaccaa
      841 gcttggcgat agcctttgtc actttttctt tcgtcgaagg cttaacagaa ccgccattca
      901 agactctgga aacggtaccg attccaacac ctgaaagtct cgcaacatct ctaatagttg
      961 gctttctcaa aaatatctct ccgtattgta cagtaatatt ggaaacg
//
LOCUS       DAOOLY010000459         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Mesotoga sp. isolate AMR_MDS_2689
            NODE_209326_length_1004_cov_4.020021, whole genome shotgun
            sequence.
ACCESSION   DAOOLY010000459 DAOOLY010000000
VERSION     DAOOLY010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864764
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mesotoga sp. (wastewater metagenome)
  ORGANISM  Mesotoga sp.
            Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
            Mesotoga.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:25:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,628
            CDSs (total)                      :: 2,610
            Genes (coding)                    :: 2,604
            CDSs (with protein)               :: 2,604
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 2 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Mesotoga sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209326_length_1004_cov_4.020021"
                     /isolate="AMR_MDS_2689"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053577"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLD15_13135"
     CDS             complement(<1..572)
                     /locus_tag="PLD15_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB64907.1"
                     /translation="MVKVVTDSSCDLPLELIESNGITFVPMNVVVDGRSYKEDIDITP
                     REFWKLMSESKTLPKTSQPSPGEFADIFNSIQSEGNTPLCITISSKLSGTYQSAQIGA
                     QLSGNRAVVFDSLAGSLSHGIQVITASRMALQGKSVDEILHTIEECRKNVRIIIPLLT
                     LENIIKGGRLSKFQGGLANILNIRIILHGV"
     gene            complement(669..>1004)
                     /locus_tag="PLD15_13140"
     CDS             complement(669..>1004)
                     /locus_tag="PLD15_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB64908.1"
                     /translation="SVDWFLPNEAEFLSLMQLEYLEEAGQIYKSDYTSRALIKMGSRG
                     SMLMGKDQCEFHHAEKTGDIIDTIGAGDAFNAGFISGLAGNGKIDIATAHTIAKDWIE
                     GKYRAYITF"
BASE COUNT          229 a          260 c          244 g          271 t
ORIGIN      
        1 tcgacgccgt gaaggattat ccttatattc agaatgtttg cgagaccgcc ctgaaacttg
       61 ctgagacggc ctcccttgat tatgttctcc agagtcagca acggaatgat gatcctgacg
      121 tttttgcgac actcttctat tgtatggagt atctcatcca cgctctttcc ctggagagcc
      181 attctggaag ctgtaataac ctggattcca tgagacagag agcctgcgag agagtcgaaa
      241 acgacggctc tgtttccact caactgcgca ccgatctgcg ccgactggta ggtaccgctc
      301 agttttgacg atatggttat gcagagcggc gtattgcctt cggactggat cgagttgaag
      361 atatcggcga actcgcccgg agaaggctgg gaggtctttg gaagcgtttt tgattcgctc
      421 atcagcttcc agaactcccg gggcgtgata tctatgtcct ctttgtatga tctgccgtcg
      481 accacgacat tcatggggac aaaggtgata ccgttagact cgatgagttc taaaggcagg
      541 tcgcaggaac tatccgttac cactttaacc atggcaatct ctcccgaatc gattattcga
      601 cgatattata actcacagat cgatcgtccc acggcctgtc gaaagactct ttcgctacgg
      661 gacagagatc agaaggtaat atacgccctg tacttgcctt ctatccagtc ttttgcgatt
      721 gtgtgagcgg tggcgatatc tatctttcca ttgccggcca ggccggaaat aaatcccgcg
      781 ttgaaggcat ctcccgcacc gatcgtatcg atgatgtccc cggttttctc cgcatggtga
      841 aactcgcatt gatccttacc catcagcatc gatcctctgg aacccatttt tatgagagcc
      901 ctgctagtgt agtcgctctt gtatatttga ccggcctctt ccagatactc taactgcatg
      961 agagacagga attcggcctc gtttggcagg aaccaatcga cgga
//
